LOCUS       DAOPYV010000001        70936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_246_length_70936_cov_11.955602, whole genome shotgun sequence.
ACCESSION   DAOPYV010000001 DAOPYV010000000
VERSION     DAOPYV010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 70936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70936
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_246_length_70936_cov_11.955602"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PKI35_00005"
     CDS             complement(<1..368)
                     /locus_tag="PKI35_00005"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNX42788.1"
                     /translation="MLSPLDVDIFVPCFIDQLYPETGFNMVKILEKFGVKVHYNPVQT
                     CCGQAAFNAGYWDEAKAVGEKFIRDFSNNRYIVSPSASCVGFVRNYYDEMFYNSALHN
                     EYHQLKKNIFEFTDFLVNIL"
     gene            complement(509..1780)
                     /locus_tag="PKI35_00010"
     CDS             complement(509..1780)
                     /locus_tag="PKI35_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNX42789.1"
                     /translation="MIVIPPATPLVRTSVHLPASKSISNRLLIIRQLSGGTLAIRNLS
                     DAGDTVLLKELLAMVNASARCRTPQTIDCRDAGTVFRFLTALLAVTPGNWFLTGSGRM
                     KQRPVGVLADALMESGADIRYTGEKEFPPLAIRGGAIKGGNSVSAPGDVSSQFISALM
                     LIAPSLPGGLNIRITGRAVSAPYIAMTAGIMQQCGADVTVASDLVRVAGKPYTPPVDG
                     LSVEADWSSAAFWYEVVALSDETEVILPGLSFNSLQGDAIAAGIFEKLGVGTTETAEG
                     ILLKKYRIRSDESFIQDFTSCPDLAQAVAVTCAGLNIPARLTGLSGLRIKETDRLSAL
                     VSELTKTGYRVHAEKGSDLVIAGNQPVSGDDKIVIHTYNDHRMAMAFAPLAFKTGKIG
                     IDNPAEVRKSYPGFWNDLKSAGFRFENFCWE"
     gene            complement(2409..3464)
                     /locus_tag="PKI35_00015"
     CDS             complement(2409..3464)
                     /locus_tag="PKI35_00015"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HNX42790.1"
                     /translation="MQDIVAPYFGPEASSAIDEVVNRHAKAGIFILVDENTKKYCYPQ
                     LAERHPSLSNARIVEIPEGEKAKNFHTAVSVWEQLSEKGALRDSLLFCLGGGVLCDVG
                     GFTASSYRRGMPFVMVPTTLMAMVDAAIGGKNGINLAGLKNQIGTFSQPESICVFPDF
                     LKTLPARHILSGFSEMIKHALIDGESFEDLKKISPTEIVSAPSAIKQSALIKMRVVKQ
                     DPLDKGIRNVLNFGHTIGHAFETWSAMHMSEPMLHGEAVAAGLICEAFISVKHCGLPI
                     SVLYEIVELVMEHFRKTIFYAPAGNELIEIMLYDKKVKQEGEINFTLLGSPGAPLVDQ
                     YPSESLVRESLHYYVNL"
     gene            4024..4821
                     /locus_tag="PKI35_00020"
     CDS             4024..4821
                     /locus_tag="PKI35_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HNX42791.1"
                     /translation="MKGLIFDIKRYCVHDGPGIRTTVFLKGCPLNCLWCHNPESRLHE
                     IQYVHRKRNIGNQEFEEEEIIGRWYTVAEVMKELEKDSLFYDESGGGITLSGGEPLMQ
                     AGFTGELASACRQRGWHVALDTCGYASRSSFEKVVPYINLYLYDLKGMNNADHLIHTG
                     VENSLILDNLISLLDGGGKVIFRYPVIPGMNDSENHLLDLMHFLMNLRSTQREIHLLP
                     YHTIQKSKYERLGLTYRLSELPAATDESLREMKAGLESIGYTVRIGG"
     gene            4885..7245
                     /locus_tag="PKI35_00025"
     CDS             4885..7245
                     /locus_tag="PKI35_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HNX42792.1"
                     /translation="MMTDRIKKLRQQSLDAVNRLTPERALLVTEFYRDNREFSIPVQR
                     ALCFKYILTHKSICINDGELIAGERGPEPKATPTYPEVNLHSMHDLEVLNSRPKVSFK
                     VDDEMRLAYENVVIPFWKGKSHRERMFAEMTPEWIRAYDAGMFTEFQEQRAPGHTVLG
                     KKIYSMGMLDIIGETDRAYAMLDIENDRDILPKKEELKAMRIAAEALILFAERHAAEL
                     DNLAASETDAGRRQELINMATICRNVPAHAPQTFHEALQYYWFVHLGVITELNPWDSF
                     NPGRLDQHLYPFYKRETEAGTLTRETAEELLQSFWIKFNNHPAPPKVGVTALESNTYT
                     DFALINLGGVQSDGSDAVNELTFLILDVIEEMRILQPSSMIQLSKKNPDSFLHRALRI
                     VKTGFGQPSIFNTDAIVQELLRQGKSVEDARNGGASGCVETGAFGTEAYILTGYFNLT
                     KVLELTLNNGHDAQTGELIGLQTGEASSFNSFDELMSAYRKQLRYFIDIKIKGENIIE
                     QLYARYLPVPFLSLLIDDCIKNGRDYTAGGARYNTSYIQGVGLGSLTDCLTALKYHVF
                     DNHKISLAEMLQALSENFTGHDKLRYDLVYNTPKWGNDDDYADDNARMIFEIFYDAVN
                     GRPSPRGATYRINMLPTTCHVYFGSKINATPDGRLAGEPLSEGISPVQGADKSGPTAV
                     LKSASKIDHIRTGGTLLNQKFTPSFFDSEESIAKLGKLVRTYFRMDGHHIQFNVVSAA
                     TLKDAQKHPEKYRDLIVRVAGYSDYFNDLGEDLQNEIIRRTEHESA"
     gene            7348..8559
                     /locus_tag="PKI35_00030"
     CDS             7348..8559
                     /locus_tag="PKI35_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42793.1"
                     /translation="MSSYVTRSLLSVLFFISLSVNGQNALIRKIFPDSAYNCRDLQYQ
                     SMQLIPLYYGNRDFDTANAILDYWAGQCSTDETIFRTRILFAIDNGTFSDSLFKNDGF
                     TYYLDVYKELANDTSGSSLYYYPYYYVDEIELLKWYKTFTDSIAFRCLTYTDLSPEER
                     FFATYYAYPSDSIYTLLFAKVYDSTALKKIASEPVYGQMENTQGHYSFSTGMWIPDQR
                     LDTLGRHPYLGAQIGLKHNRYLADLVLDLRFGSSPNTYQVVIDGETWDTEKFFEMSWR
                     LDFGYELFNIRRTEMLFLGGLGYDCINAVNETNDPDNEDDDVSKYLHSFNMNLGGSYR
                     YYLNNNHYLAVTGKYHVLNFRNKGGTDLKGNALTLSLEYGFGMNRWLNDRNTILRKKT
                     KNYRQQVRQSL"
     gene            complement(8621..9733)
                     /locus_tag="PKI35_00035"
     CDS             complement(8621..9733)
                     /locus_tag="PKI35_00035"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HNX42794.1"
                     /translation="MNRTFLLIFVFSAFLLLKARPQEPLVPVQPRHYVCYRTSAPLNI
                     DGKLNEADWGKAPWTEYFGDIEGSAKPEPRLKTRAKMLWDDNYLYVAAFLEEPHVWAT
                     LTERESVIFYDPDFEIFIDPDGDTHNYLEYEMNAFNTQWDLLITKPYRDDVVWNVAVD
                     NWNYNGMKSGAFVDGTINNPCDTDSGWYVEVALPLDAISEVNTGGKRPGAGDQIRINF
                     SRVEWTMDIVNGRYVKRTRLIDGKEKPLPEDNWVWSPQGVIAMHQPETWGFLQFSAKT
                     AGSGTDIYLENPDYSIMQALRKLYFREKAWFGKNGSYTANLKSLGFSDYQVNGKPFRP
                     EISLTKSLYEAVANGSAAGTLWHITQDGKIWKTVGK"
     gene            10079..10225
                     /locus_tag="PKI35_00040"
     CDS             10079..10225
                     /locus_tag="PKI35_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42795.1"
                     /translation="MSHEFHECNHEFHEMMTTKYKTQNAERKTQKAELTTQDSELKRP
                     DEEL"
     gene            10493..13441
                     /locus_tag="PKI35_00045"
     CDS             10493..13441
                     /locus_tag="PKI35_00045"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNX42796.1"
                     /translation="MKNSVAGLASILALILLSFTGFAQKAPVDYVNPFIGTGGHGHTY
                     PGATMPFGMVQLSPDTRLEGWDGCGGYHYDDEYVYGFSHTHLSGTGVPDLCDILIMPT
                     TGKARFNNGYDGKPGYRSHFSHKNETATPGYYKVQLDDYGITAELTATERAGFHKYTF
                     PASDSANILIDLFHRDDAKEASIQVKGNNEVEGVRYSEGWANNQVVYFVAQFSKPFSG
                     VSLASNNVVLPERLALDKRKNVKAAFHFKTAEGETIYVKVGISAVSIAGARKNLEKEI
                     PGWDFDQVRSNARQAWNKELSKIIVEGGTDDQKAVFYTSLYHTMVVPNTFMDVDGIYR
                     GRDFKNHKAEGFTNYSVFSLWDTYRAYHPLMTIIDQKRTLDYIRTFLTQYDQGGLLPV
                     WELDANETWCMIGYHSIPVIVDAYMKGIKDFDVNKAFEAMKKSATRDNPGLKALDQYG
                     FIPSDREAESVSKTLEYAYDDWCIAQFARSIGKKDEYKYFIHRAQNYKNLYDPSTGNM
                     RARINGGWFTPFDPTEVNYNFTEANSWQYSFTATQDITGLSNLMGGKDKLAAKLDALF
                     SASTQTTGRDQSDITGLIGQYAHGNEPSHHIAYLYPFAGQPWKTQELVHRIMNEFYKN
                     NPDGLIGNEDCGQMSAWAVLSAMGIYAVNPGSNFYVIGTPWFDKVTINLENGKQFVIK
                     ANNISPANYYIQSATLNGKKHNASYLKHADIMKGGELVFTMGSQPNTEWGVGKKNEPV
                     TAITDNLVLPAPYVKDAVKTFSGAKTIELAGPAGATVMVMTSGADRIGENTPYTAPIE
                     LTETSKVKFFAQQKKLVSPTVTAEFIKSEINKDCKLITAPAQQYSGNGGLTLVDNIRG
                     NQDFRVGGWLGFEKENFEAVVDLRESKEVSRLSAGFLQDENSWVFMPTQVEFFLSDDG
                     VNYKSAGVVKNDVPWQKTGAFTRDFNLNITAAKARYVKVIGTNRGTCPEGHKGNPGAA
                     WIFVDEITVE"
     gene            13575..16229
                     /locus_tag="PKI35_00050"
     CDS             13575..16229
                     /locus_tag="PKI35_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNX42797.1"
                     /translation="MIIRKILPALALTVLLSYSGQAQLIRNSATLKDMQQMFEKQKQL
                     ASGRSNELFGIFKTPLTGDEKQALQYLYAYMPLSDLADYNGKFFLKAVRISLKARNEM
                     PWGKKAPEDVFLHFVLPMRINNENLDTFRVAMYPELKARIRGMNMYDAALEVNHWCHE
                     KVTYRGSDERTSSPLASIRTSFGRCGEESTFTVTALRTVGIPARQVYTPRWAHCDDNH
                     AWVEVWVDGKWYFLGACEPEPALNMGWFAEPARRAMLVHTRAYGHYNGNEPVIENRER
                     FAELNLIANYAETKPIFVRVVDASGKPVKDATVEFQLYNYAEFYPIARQTTDSKGVCG
                     ITTGLGDLLLWATLDGKFDYRKITVESTDTVVLQPGRPHAAGEIFDLVPPVEKTPLDS
                     KVTDLQRSRNTQRLNHEDSIRSAYMATFTDSVQAVAFAGKLGISREKAVKYLTLSYGN
                     SKEIKSFLQNTPAGKMQWAMYMLGVITEKDIRDTRESILRDHLDNAFTYAGSNEPADR
                     EFFARYVMSGRIANEMMLPWHAFLQKKFNAEFAAKAQKDINIIIDWIKTNIRIDNTAN
                     LHSRSPLSPRGVYELRVADKRSRDIFFVALCRSMAIPARINPATGIPQYRQNDDWQNV
                     AFEPVAENIAQKGFIRFVNGNPATDPKYYINFTVARLSDGVYRTLELAEGKSVSDFTE
                     PLSVEAGAYYLVTGNRQADGSVLSSITFFDVPAGKTIDLEVTMRESFEEQKPWGAIVP
                     DNFSLENYVDKKTLKMSDVMNHKGVILVWIDPDKEPSKHVMADIPAVRSVIEKWNGGL
                     VFLLTEGRITQAFNPETYKDLPAHSIFAYDKQGKLLDAVGKIKGRKLDASMPVIMISD
                     TGGNLFYFSEGYKIGIGEQLAKEINRMK"
     gene            complement(16262..17908)
                     /locus_tag="PKI35_00055"
     CDS             complement(16262..17908)
                     /locus_tag="PKI35_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX42798.1"
                     /translation="MLQENFVKYIEDSLKKNWELPALADYNGESYKYSDIAVQITRLH
                     EFLRLAGVSQQDKVALVGKNSARWCITYLAIVTYGAVIVPILPDFKPEDLHGILNHSD
                     AMMLFADEIFAKDLRPEQLPSVKGIFSVFDYAMIHSGNDQIKAAYEQSSVLKPVSESS
                     LTADKLAFDPVPNDRLAAISYTSGTAGFVKGVMLQHNSLAANIKYAQHNMPLQPGDKI
                     VSFLPLAHAFGAAFEFLFPFTLGCSITILTKTPSPQIALQAFQEVRPALVLSVPLVIE
                     KIFKKQIQPVISKWYMKVLLLIPGVRGKIHTKIREKLVSAFGGQFHEIVIGGAPFNKQ
                     VERFFHKIKFPFTVGYGMTECGPLICYQNWRETRIGASGKVVDTLELKIDSPDPRNIP
                     GEISVKGDNVMLGYYKNPELTAEIIDANGWLHTGDLGLVDAEGRIYLKGRSKSMILGP
                     NGKNIYPDEIENRLNNRHGIGESLIVSRNNKLIALLYPDQDVVAHEKLAREDLEKLFE
                     HHRKAVNKQVPHFISIAGFEIQDKEFAKTPKRSIKRFLYS"
     gene            18196..18891
                     /locus_tag="PKI35_00060"
     CDS             18196..18891
                     /locus_tag="PKI35_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42799.1"
                     /translation="MKPFSYLLPAILAAGMILLITSCDDTGNKAPSISSVRINPDSVN
                     AGGTVKVTVNAVDPEQDPITYLYEVSGGSITPDGSYGYWSLPSAPGTATVEVTATDSE
                     GNSSSPTTISVTVLAPVTQITGYAKLDAGYTGNLEGSVVHLETYGAGLIIKTITATGS
                     GSGIVFNLAGIDPGTYQLIIWKDTDHSGNPNSGDYVGWYGSGGPLSPDFDPIEITGGQ
                     NFICNISMTHPAK"
     gene            19161..19895
                     /locus_tag="PKI35_00065"
     CDS             19161..19895
                     /locus_tag="PKI35_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HNX42800.1"
                     /translation="MILEIAVYSLEAAITAQKAGAGRIELCAAPAEGGLTPSAATLRL
                     ARKYLKIPIHVMIRPREGDFCYSDLEFNAMLLDIQAAKIAGMDGIVAGILSKDGTVDT
                     GRMRVLAEAAAPMPVTFHRAFDMTCDLFDSLEKIVGCGIKRILTSGGRQTACEALPVI
                     RRLAEAANGRISIMPGSGINEHNIREVIDRTGVSEIHLSAKMHIPGNMEYKNTAVAMG
                     GNVIIPEYDLLLPDADAISRIRKLID"
     gene            19928..20764
                     /locus_tag="PKI35_00070"
     CDS             19928..20764
                     /locus_tag="PKI35_00070"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNX42801.1"
                     /translation="MEETSIPVLYDTRLFTRIWSPARFPAGIVCLVHGLSDHGGRFVH
                     VAEALAAKGYAFIAPDLRGNGRSPGRRGHFNSLQEVMTDLKLILKDARDRFPGLPVFL
                     YGQSMGGSLVINFVLRFPEEVDGIISSSPWLRLAKPPSGFTVAAASLLKPFFPSALVP
                     NGIKSADLCHDESVQHAYEIDPLIHWKISVRTFFIIQKYGEWAIQHAGMLKKPVLLMH
                     GDADRITSYKASRDFASASNNHLNFKGWPGLFHELHNEPARDEVLAYVSDWLDRCRFR
                     RL"
     gene            complement(20819..21448)
                     /locus_tag="PKI35_00075"
     CDS             complement(20819..21448)
                     /locus_tag="PKI35_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42802.1"
                     /translation="MKTLTLILLSGILSINHPFNATADGDPHNKTSYTSPFFFNAFTD
                     DLQNSFSGMLKPDEEPFVNDIPFSTSGITADYLRNLKLKSIEIPEMSPEFYIDDIPFN
                     TENIATLYRLKASDVSGILNLKDESPAPDFPFNTAIIAGSAASGKLPLVLQPEQYADD
                     IPFDTEEISKQSLQREKITVNRGITASGLTDTPDRFPYEGSLYRFDADK"
     gene            22015..22404
                     /locus_tag="PKI35_00080"
     CDS             22015..22404
                     /locus_tag="PKI35_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42803.1"
                     /translation="MKKIVVTLAVLFLSGTIAMAQTQKPVEKKEPVKTTTMKQEAAKP
                     VAGQKTANVQTKSQSVATQGVIKAEPAKAAQASTATKAVVHKHHKKAHQASGVSTAKS
                     TPIAAPATKTAPAKDVKNTSNVQPIKK"
     gene            complement(22578..23831)
                     /gene="hutI"
                     /locus_tag="PKI35_00085"
     CDS             complement(22578..23831)
                     /gene="hutI"
                     /locus_tag="PKI35_00085"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNX42804.1"
                     /translation="MKILITNIKELVQVENKPRMMVCGPEMGHLGTIRNAFLLIEKGK
                     ISDFGRMEDLFLPETREGRQLKIIDAAGKMVFPCFCDSHTHLVYAGSREIEYVDKIRG
                     LSYEEIARRGGGILNSAAKLHDAAETELVAQSLERLEEMMRYGTGAVEIKSGYGLNTQ
                     DELKMLRVIRSLKEFSPVTIKATFLGAHAVPAEYKGNQSEYVDLIINEMIPQVAAEDL
                     ADYIDVFCDRGFFTIEDTERMLMAGMKYGLRPKIHANEMDRSGGIQVGVKYNALSVDH
                     LEFTGEDEIKALLDTETMPTILPGAAFFLNMPLAPARRMIDAGLPVALASDYNPGSSP
                     SGNMQLILSMGCILYRLTPEEAVNATTLNTAYAMGVSEELGSIAVGKKANVFITNAIP
                     SVEFMPYAFGSNKVDTVILNGRIVA"
     gene            24021..26141
                     /locus_tag="PKI35_00090"
     CDS             24021..26141
                     /locus_tag="PKI35_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNX42805.1"
                     /translation="MNLRKFTLLITLIMALASVEAAIVDKEAARTVAGNFIKERIYHH
                     QINWQASGLQMTDVLTYTIDGVPAYYVFSNNGTGFIIISADDRITPVLGYSEKGVYTE
                     IGKATNFDYFVHCYADQVKWVRESVYDTPADVSASWNNYLNGNLDYTPNSTTDAGPLL
                     QSIWNQSPLYNEFCPEDEDGPGGHVVTGCVATAMSQIMYYYRFPLQGSGSRSYYMPDY
                     GTISANFGETYYDWDQMLNELTNGSGQCIPAVALLNFHAGVSVSMNYGPDASGAQSDD
                     VPYALKTYFRYSSSVTYQEKGSMTQTQWENQFTPNLDEKKPVYMSGHDPAQGGHAWVC
                     DGYQVNGTSKLFHMNFGWGGYNDGYYSLSNPQGFTNSQACVKNIYPGAAYYPYSCSQD
                     TLTGAIGSFEDGSSGTANYGSNLGCTWLIAPADSVKTITLSFSAFDVDASDMLYIYDG
                     SDNTAPLLASYTGSTLPAEVVSTGNRMFVVFTTDGANESTGWHAEYYSQFPSYCGGTI
                     TLTDPSGTFSDGSGNSSYNNNSLCKWKIVPPYATGLTLSFNSFNLTEGDELMVYSTGG
                     TTGSTLMGTFTGNQIPDPVVSTGTGFLVMFKSDTYNPGAGFEAQYTVGNVGLDEQAGI
                     NGIVIAPNPATKYTSVRFYNKSDEPFRMNIVDMAGKELYHESFAGTSGNFVKNIDLTG
                     FNKGMYFLIIRTSEGVTTRKFIVK"
     gene            26267..26620
                     /locus_tag="PKI35_00095"
     CDS             26267..26620
                     /locus_tag="PKI35_00095"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNX42806.1"
                     /translation="MKPGLRRYLLFSFNSLEAIIWLTALILLAATANTGTHYTLCPLK
                     YFGFEFCPGCGLGRSIALILHGQFIGSLRMHPLGFPALALLLWRILSVFTNAFRLSRV
                     QPIQNSGQQANPSTI"
     gene            26651..26842
                     /locus_tag="PKI35_00100"
     CDS             26651..26842
                     /locus_tag="PKI35_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42807.1"
                     /translation="MSTPLYQLLPELDMQEMGFIQGITKNYTEQQLLNFANMYRIRRR
                     DPQIILLTALAGFIGIAGI"
     gene            26867..27010
                     /locus_tag="PKI35_00105"
     CDS             26867..27010
                     /locus_tag="PKI35_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42808.1"
                     /translation="MGILYLLTGGLCLIGTIVDLVNHRQLALEYNVQVAHEVASMVGQ
                     MPA"
     gene            27133..29220
                     /locus_tag="PKI35_00110"
     CDS             27133..29220
                     /locus_tag="PKI35_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNX42809.1"
                     /translation="MRYILPIFVISFFFAAQLFAKEVTVSDARKVAGTIIREKAPSSN
                     QDFTLSENMLTVSMNGLPVYYVADLSGNGFIIVSADDVVMPVLGYSFTGGYDENNLPE
                     NFTAWMQGYAEQINYARQHNLQADDKITGLWKSYLSGQQPEGQRATTGVAPMLTCTWN
                     QGAPYNLLCPADSKGPGGHVYAGCVATAMAQITYYWRWPLQGTGSHGYTWGNYGYLFA
                     DFGNTEYHWEEMANSSIGFNYEMAQLQSHLGISVNMMYSPDGSGAYSEDAATALKTYF
                     GYSDDLTLVYKDDYTDEQWGNLMRQQLDAGQPMYYDGYGTGGHAFNLDGYSDDLYFHF
                     NWGWGGTANGYFLLSNLNPAGNNFSQGQGAIINFVPNSTYPYYCTEANTLTANYGSLE
                     DGSGPLQPYLDNTGCGWLIAPQDSIRDLQLTFHRFNLEENHDFLTIYDGSDDTAPVLA
                     TLTGQSLPPVITATDNKMFVSFTSDNGGTDNGWFASYSAQTVSFCPGVTTLTEPMGTF
                     TDGSGTYDYHNNSVCKYRIMPPDARNITLYFNEFSTFDENDFLLIIDINTGSTLYQLS
                     GPEIPGPLYLNTGKVLILFKTDPSNTASGWNITYQADTYNGSENPRENSLFVYPNPAS
                     GLLNISFPCFPGEKQVLTLTNAAGHEVLARSVSSGNTLADERIDVSGFASGIYVLRLI
                     NGKSVITRKVVIE"
     gene            29483..31603
                     /locus_tag="PKI35_00115"
     CDS             29483..31603
                     /locus_tag="PKI35_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNX42810.1"
                     /translation="MKNFLFTVMLLSIVAAANCGIVTRETAGRVAANFCYESCPAARQ
                     SGYCSIKTVQIFEKDSGAPLYYICTLNTGGFVIVSASDNTVPVLAYSFDNSYDPENIS
                     CCFSSWMKHYENQIHQLIISGNEAVPAISEEWKRLASDNPENLRDNTGLLSVAPLLVS
                     KWNQGSPYNAMCPPDPAGPGGHCYAGCVATAMGQLLYYYRYPQQGAGSYAYYLPDYGT
                     ISADFGSATYQWDAMPSYLSRENDAVATLLFHLGVSVDMDYGPDGSGMWNHKAAYSLR
                     TYFGYGPETKYYFRDSTNLDWDSILVASLDQRKPLYYAGWEGVQSDNGHAFVCDGYQG
                     TGYFHFNWGWGGSNDGYFYLDNLTPGGNNFNYAQEVIPLFPDTLSSDYPPYCSGNKTL
                     SSIDGSVEDGSGWFRYQPGSDCSWLIQPDDAEHDSVSNIKLIFNAIDTDTSDSIVVYD
                     GDTTTATVLGSFSGNTLPAEITSGSDRLLVTFSSNASNNLGGWIADYRSTLPVYCSGQ
                     TTLTATSGSFSDGSGDKRYNNNSVCKWRIEPPGADFVTLWFTSFSTADTLDKVIITDL
                     VTHEQLGEFSGSEMPPPVTATSGKMLLIFFTSNTGTGEGWDASYVSSGAGEGEPFANS
                     EDIFLAPNPANDNTFLYYQCKQPGLVSLEIKSLTGKTIRSWDYQSVTGMNKIPVGIED
                     LSSSAYVLFLTSKNMKKAVRLIVN"
     gene            31839..33296
                     /locus_tag="PKI35_00120"
     CDS             31839..33296
                     /locus_tag="PKI35_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX42811.1"
                     /translation="MRKYALLAVIIFLTAAANFTTAQNSDHKWALGINWNWEDFHTVK
                     RDFPDQFKYTRWQGYKFPSAISLGRYINPSFNVFGAFGLSKLEKESMAETMVNQPLSD
                     DKFWTGDLNLAYKFANGYILKETSWFDPYLYLGLGASSIKDKDQEDGKTTYFKGDLGL
                     GMTFWATEWLGLNVQGAYDYMITPKLNDERRSDYMHYTAGLKFRFGAPKDTDGDGVPD
                     KTDRCPETPGKVELMGCPDKDNDGIADLDDACPDVAGKPEFKGCPDTDGDGIIDKDDA
                     CPTEAGLKEFNGCPDKDGDGIIDKDDRCPDVKGLKELKGCPDKDGDGIADIDDRCPDV
                     KGLKNLKGCPDTDGDGIADIDDKCPDVAGTLANNGCPEVKKFELYKVVYFGCDKSVVL
                     TKYTKDLNEVADILKANADVAVSVEGYADSQGPDAYNMKLSEKRADYVINYLVKKGIS
                     KARLNKAFFGESNPAGDNKTAAGRALNRRVEIKTK"
     gene            complement(33974..35665)
                     /locus_tag="PKI35_00125"
     CDS             complement(33974..35665)
                     /locus_tag="PKI35_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX42812.1"
                     /translation="MKILNAFLITGLMIAGLSLRAQDNGNAAKLLDMEQYEKAKSVLK
                     ADLKTMNSAESWYYLGKIYARQYNADSANYCFSRLAAADPKSRLIMVGQAISENLAGR
                     NQEAMLLLQKTRKEAAMAKETKTLIEVAENMVMAGDTCVWTDVLTAALSIDKKSVESY
                     NVAGKLYLILAGKYNYPIYFGKASGRFEQALYIQPDNVEALTGLADIYFGTRNYEAAE
                     EKYSLALAKDPLYIPALKGAGELYYTLGKYDKAGEMYGKYIGLAEYSRKDMARYISIL
                     YFNKEYSRAYELINTLLPGEPENPVLLRLKGYTAYELNRDAEGLEAMKKFFEVRSVVD
                     TNKVIFTDYENYGRLLARTGNDTLAVSNLAKAFEMDTTQYDLLEDVAKLYDKQKNYPA
                     SINYLEKFIEAKKGKVQSSVYFNLGKDYQLLANQFSGTSDSASRKPYLIKADSAFSRV
                     VVISPNSYLGYQWRARVLSGLDPETIQGLAKSDYEKTIAILELKNDRQKYSTDLIEAY
                     RYMGYYYYLQFEEAKTAKDDTLKETAKTNSRSYWLKVLELDPKNDVATKAISALK"
     gene            complement(35726..36670)
                     /locus_tag="PKI35_00130"
     CDS             complement(35726..36670)
                     /locus_tag="PKI35_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNX42813.1"
                     /translation="MKYSFIALTILLLLTATGCKPKKAKDKYTDTPTTGQITIAVDET
                     FQPVIEPELPVFHAMYKGASIKAAYVPEVDAINMLLRDSVRLAIVSRPLSAKEINFFH
                     SKKFFPHEVQLAVDGIAVLVNPSNPDTLFSVNTIRKILLSEITSWKQLNRKSPLGKIK
                     VLFDNPNSSIVRFMVDSVTRTGQLGDQLSALQYNLDVVDYVSKNPDAIGLIGVSWVSN
                     SRDPKCLSFLQKVKVAGISSEDPALPETSYQPFQAYIATGKYPFTRFVYALLTEPRAG
                     LGSGFVSFVSCDKGQRIIQKTGVLPITQPVRIVHVVEE"
     gene            complement(36933..37760)
                     /locus_tag="PKI35_00135"
     CDS             complement(36933..37760)
                     /locus_tag="PKI35_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNX42814.1"
                     /translation="MAKINIFSDDWCNLVFESRPKEYGAYLMRSLSSKRHRIAIITTI
                     ILFLLVFTLPGLIKRIIPAEKETNVEVTTLSNIKLEKSKTKEIEQPKIEQELPQKVKS
                     TIKFTPPVIKDDAEVKDEEVMKTQEEVSESKLSISTADVKGNSQDADAVDLADLQQQQ
                     TQVVEEEEEEKPFIVVEQIPEFPGGVSSMNKFLKDNIRYPQIARESGITGTVFTTFVV
                     SKTGKISDVKILRGIGGGCDEEAIRVIKAMPAWIPGKQNGRAVPVQFNLPIKFTLAG"
     gene            complement(37772..38407)
                     /locus_tag="PKI35_00140"
     CDS             complement(37772..38407)
                     /locus_tag="PKI35_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX42815.1"
                     /translation="MAELNQDAGSHKKGGKKRSKKMNPRVDLTPMVDLGFLLITFFML
                     ATSLIKPQTMEITMPSDKKVDEEDKTVAKASQAISLILAKDNVVYYYFGKTEGAKLLK
                     TDYSANGLRKILLGKNYDVAKQVEDLKSEKTKTHMNDDDYQKRLSEIKANKNAPVVRI
                     MATDDANYKNLVDALDEMNICSISRYAVVNIEAPDQDLLKAQVPGNQLSKN"
     gene            complement(38433..39041)
                     /locus_tag="PKI35_00145"
     CDS             complement(38433..39041)
                     /locus_tag="PKI35_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX42816.1"
                     /translation="MPKAKIKRKSTVVDMTAMCDVAFLLLTFFMLTSNFVKKEPVLVS
                     TPSSVSEIKIPETNVMTILIDKTGKVYFGIDGQENRQLLIEKMAETYKVNLTPEEIKC
                     YTVLDLVGNPMQALKTYLSLKPEVRDLPQNNLGIPADSTNNEFKDWVKAAREINPDLR
                     LAIKADETTPYPVIRKVMTSLQELDENRYNLITRLEGAPGKE"
     gene            complement(39069..39881)
                     /locus_tag="PKI35_00150"
     CDS             complement(39069..39881)
                     /locus_tag="PKI35_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX42817.1"
                     /translation="MKKNRRSKSRKFNLGFATFVIPVALIVAIVIFKFIMGNPANFQG
                     NDPANAALPGNFYGIIYKGGFIVPILMTLLLTVITFVIERFIAINKATGTGNVADFVQ
                     IVKDDLKKNEIDGAMTECDVQKGSVANVIKTVLLRYRTLEKDTTMAKDQKLSALQSEV
                     EESTSLELPALEENLVFLAVITSLGTLMGLLGTVLGMIRAFAALANAGAPDSVALAQG
                     ISEALVNTAFGIGTAAFALIFYNFFTTKIDKLTYSIDEAGFSLVQTYAANHA"
     gene            complement(40031..40450)
                     /locus_tag="PKI35_00155"
     CDS             complement(40031..40450)
                     /locus_tag="PKI35_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42818.1"
                     /translation="MRRKCTLGMRMLLISLFLLAGYSDSRPSFIQNLAERVQVSQTRN
                     SSGDLAQSTMIVDQMTPEGTSGSFAEVPVVVTCILPEYKSDTRSETKYRNLSNFFVSN
                     IQSLPICGFNKDQKSKGFTEIPSAHAISGSLSYSLRV"
     gene            complement(40699..41142)
                     /locus_tag="PKI35_00160"
     CDS             complement(40699..41142)
                     /locus_tag="PKI35_00160"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNX42819.1"
                     /translation="MKPEVSHQGRIVEMDDQKISVQIISLSACATCRAKGMCSASDMT
                     EKLIEVKNEHKYVFHQGDHVNLVMKQSMGNKAVILGYFLPFVLLLITLLISSQYTGEL
                     YAGLLSLGILVPYYILLYLLRGRLSKSFNFKIEPLNAETGEESKS"
     gene            complement(41343..42569)
                     /gene="hydF"
                     /locus_tag="PKI35_00165"
     CDS             complement(41343..42569)
                     /gene="hydF"
                     /locus_tag="PKI35_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HNX42820.1"
                     /translation="MKSKTLRGHIGIFGRRNNGKSSLINAITGQDVAIVSDIAGTTTD
                     PVRKTIEITGLGPVVLIDTAGIDDIGLLGEKRVAKTREVLKTIDLAILIITHNTFGAF
                     EKELVSEFKEFDVPFFIVHNKADIAPIYSETQRLIRTSLSTDVLDFSSTSNPQVERLI
                     SLIRKHLPESSLNQNTLIGDLIAYGDTVLLVTPIDIEAPEGRLILPQVQVIRDILDND
                     GIAVVCKEREVDAYIRKMDPKPSLVITDSQVFPKADASIPKYIPLTSFSIVLARQKGD
                     FPNYLKGTPHISKLKDGDKVLILESCSHHVSCDDIGRVKIPRWLSNFTGKKLEYTVVA
                     GLNELPAAIKDYAIVIQCGGCMITRKQLINRLKPAVDAGIPVTNYGMAIAYVHGIYNR
                     AVAPFVRMMNVSEDYL"
     gene            complement(42591..44048)
                     /locus_tag="PKI35_00170"
     CDS             complement(42591..44048)
                     /locus_tag="PKI35_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNX42821.1"
                     /translation="MNEKITNLRSEKDSLGSLEVPADALYGIHSLRAISNFPDDTRFP
                     PEWYKAVGLVKQACYLTYQKFKTAVRSKYPDAVFNFPLIDDSVIKAMTEAASEVAAGG
                     YSGQFIVPAISGGAGTSINMNVNEIIANAALIKLGHKPGDYSVVHPIESANIFQSTND
                     VIPTSLKIAIMQLLDELENSINMLRQSVEALEGEYRNELRIAYTQMQEAVPSSYGKLF
                     STYSEALSRDWWRVSKCFERIKIINLGGSAIGTGITVPQFFIMEVVPVLQQLTGLPVA
                     RGENLPDATNNLDSFVEVHAILKSFAVNIEKIVSDLRLLASDVGAAEVIIPPRQVGSS
                     IMPGKINPVIPEFAISVAHKIYSNDMLISSLSAQGCLDLNAYIPVIGNAMIESLKLLI
                     GVSRTLRENFFEGLAVRKGTAATRLFYSPSITTALVPYIGYDRSAELARLMKEKSISI
                     VDANKQLNIVSKEILERALKPENLLKTGYSLNDIL"
     gene            complement(44071..44787)
                     /locus_tag="PKI35_00175"
     CDS             complement(44071..44787)
                     /locus_tag="PKI35_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42822.1"
                     /translation="MLHARLFRVFCILAAFVVLLGACDPARQKRLEAAKNDSLVAALD
                     SVESGLLTLEKKYHSGKLTYQGYIRQKIVYDKAYENISAQLGGLHKAYELPRWALQLG
                     LIDPRGMMLDSVLSQQTSVDRPDEGFNSVTLVYISSYDTAKMWADSIASVAHLSEVND
                     YLVKAKKVPAIKAGSNRGITYLNYNLRDNNKEYLISVQAGPEGILTITATNMKQLNEK
                     LSGYKALKERTSLKKQNKKQ"
     gene            45010..46023
                     /locus_tag="PKI35_00180"
     CDS             45010..46023
                     /locus_tag="PKI35_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX42823.1"
                     /translation="MNYRYLGNSGIKVSEICFGVMTFTGEKPTETGSTNQKEADELTG
                     LALDHGINFFDTADVYANGISETILGKALGVRRKDAVVATKVRFAMSDKPNDTGLSRH
                     HILESCHASLRRLGTDYIDLYQIHSYDPGTPLEETIRALDDLVRAGKVRYIGCSNLTA
                     WQTMKAQAVSEKLNLEKFITTQLYYSIGARDVEQELVPLCLDQKLGILCWSPLSGGFF
                     TGKYRQGMPLPEGTRRSNRKASGMKYWPVDEARGYEILGVLDKSALKNGKTTAQSAIN
                     WLLQKPAVASVIIGARNKAQLLENIASSGWKLPEEDITALDEISKPVIPYPIWHQNYS
                     DKR"
     gene            complement(46036..47727)
                     /gene="ftcD"
                     /locus_tag="PKI35_00185"
     CDS             complement(46036..47727)
                     /gene="ftcD"
                     /locus_tag="PKI35_00185"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNX42824.1"
                     /translation="MKQLIECVPNFSEGRNMAVIKQITDQIETVEGVRLLDVDPGAAT
                     NRTVVTFVGEPEAVIEAAFQAVKKASEVIDMRFHKGEHPRFGATDVCPLVPVAGISME
                     ETVVYAHKLAERIGNELGIPVYCYESAAFTPERRNLANNRSGEYEGLPKKLSDPHWKP
                     DFGPAGFNERVATTGATAVGARDFLVAYNINLNTTSTRRANAVAFDIREKGRPVREGN
                     AVTGKKKKDGNGKEVWIPGSLKACKAIGWYIEEYGIAQVSINLTNISITPVHVAFEEA
                     CKKAQERGLRVTGSELVGLIPLKAILDAGKYFLRKQQRSVGVSESELVKIAVKSLGLD
                     DLKPFNPHEKIIEYLLIDKSQKKLVDLSVAGFADETASESPAPGGGSVSATMGALGIS
                     LATMVANLSSHKPGWDERWEEFSNWAEKGQKIKDELLFLVDEDTRAFNRIMDALGLPK
                     GSDTEKAARENAMQAATRYAIEIPFRVMKTSFESMEVIKAMAETGNPNSVSDAGVGAL
                     AARSAVMGAFLNVKINASGLKDKDFVQKILTEGAEIQAKAIEAENEILKIVESKI"
     gene            48058..49122
                     /locus_tag="PKI35_00190"
     CDS             48058..49122
                     /locus_tag="PKI35_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNX42825.1"
                     /translation="MKISLITIAGLLLVYVGISIISLLAEPSQNSNLYETHDGTAVVI
                     TGAAARIVQEVALLEQLQQAGWLKNVCFISGASSGALNTVMLNAILTHKFSWQRYRQL
                     LFGLKSDDVFSREGNSLPFDTNPLKNLLTSVICDSLGYDSIGNLPLASSISVTDLEFI
                     PLSLKTSRLSNRKINQESKPGLNLVDVLMASTAIPVLFPSVRLRPSPTIPSANFIDGG
                     VSEDHIPYRAVLQFEKYRKMNVDTLIIVSRKCDTKLQIDTEMRQLGVKEKDRILLEKA
                     GIRLENLAKDGFIKQMKELKKFYPDLAAHTYVYIPDFQQNFRMMDFGRMEEQYDITAK
                     WAESHSPVPLDEYLTSVDNQ"
     gene            49294..51270
                     /locus_tag="PKI35_00195"
     CDS             49294..51270
                     /locus_tag="PKI35_00195"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HNX42826.1"
                     /translation="MHNLLKITLTAVFAILLSQTYAGRKERTIKTTSPDGHIVISLTI
                     ADMPVITVTCNGQEIMSPSKQGLAIREHPEAYLNPIVKKVKYRAVDEVIVPAVAEKRQ
                     QIPDIYNETEIWFRSNCGIKLRAYNDGAAWRFITRFSDSVTVNQEHVTLRMAASDSVY
                     FGEEDSFLSHSERLYPLKSASEIKGKQMCVIPAVFRKSTGIIAAFTEADLLDYPGLYL
                     TGTSEGSAVFKGIQPQAVATESVSKDKRSYVVDTRHDYIARTAGSREYPWRVFGIATN
                     DAQLLGNDIVYRLGSPCKLTETDWIKPGKVAWDWWNDWNIYDVPFKAGINTETYKYYI
                     DFASKNKLEYVIFDEGWSANDDLDKINPDMDMEALFAYAKEKNVKIILWVTGRALQDK
                     FTSSLDKFEKWGCAGIKMDFMIRDDQQMVNFYEKVAAETARRHMLADFHGSFKPTGLS
                     RTYPNQLTREGVRGLENSKWSNEITPAHDCTLPFTRMFAGPMDYTPGAMTNATKKNFA
                     ISWSEPMSQGTRCHELAKYVIFESPLQMLCDAPTNYMGETEAMNFLSPVPTTWDETIG
                     LGARIGQYVAAARRHGDTWYIGAMTDWSARSLEIGLSFLGEGTFKLETWYDGPNADRK
                     GEDFLTGNKIVDKTTNLKVEMAPGGGYVARITKM"
     gene            51327..53522
                     /locus_tag="PKI35_00200"
     CDS             51327..53522
                     /locus_tag="PKI35_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42827.1"
                     /translation="MLRFLIMLLTVSGIAECSFAQQNIEQHMMLLPQKPASVSGPDWL
                     LDGSAYKAGVYQGQSPKDLVISNGLVERTFRISPSLACYSFRNLITGQEMIRAVKPEA
                     MLTIGGNIVEAGGLSGQFEYGYLKTEWLNSFTASGSFTLTDFSVNDIKPFLEWQPVRW
                     IPDHTWPPKGKEITFYLEGSSPVTTGIEIAIHYEIYDGFPLVSKWLEVVNNRPEAISV
                     DRVTSEVLAFVEEEVSVDKMNQWKTPNIFIASNYSFMGMTGPSADRVTSWLADSAYTT
                     QVNYERKTPCLLVCRPPLGPGIKLAYQQHFTSLRIWELCYDSYDRERKGLSERKMYRT
                     ISPWALENPIFMHLTSSDPDAIKQAVDQCRQTGFEMIILSFGSGLNMESTDTAYIRKF
                     RDLNAYCRNSGIELGGYSLLASRRINDEEDVINPVTGKTGGAVFGNSPCLGSNWGNRY
                     FENIRHFIGNTGFSLLEHDGSYPGDVCASTKHPGHAGLEDSQWRQWEKITGFYAWCRS
                     RDIYLNVPDWYFLAGSNKTGIGYRETNWSLPRERQLVLGRQNLYDGTWGKTPSMGWTF
                     VPLQEYQGGGEAATLEPLSQHLKEYEAHLFQNFGAGVQACYRGSRLFDTEETRQLVAG
                     WVDWYKKYRLILNSDIIHLRRADGRDWDGFMHVNPALKQKAIILLFNPLKQPVERTVT
                     IPLYYSGLTGKASIREKEGPGTVVLLNSACEANISVKLPAEGFTWLVVE"
     gene            53760..54392
                     /locus_tag="PKI35_00205"
     CDS             53760..54392
                     /locus_tag="PKI35_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNX42828.1"
                     /translation="MPIVTDSHLRAFVDAAHRIGTHQLLLCSSGNLSWRINEQTILIS
                     QTGSWLPYMTKDQICIANMSDGVVINNVKPSMDSGFHFGVLRARKDVNVVLHFQSLYG
                     TTLASLPNPPINFNVINEVPQFIGPIAMLDYICPGSPEIGRMVTDTMRNNNLLVLRNH
                     GQVVVGKDFDEVIQRALFFELACGIIMRSNNEAVRLTEDQICEMNRYMKK"
     gene            54478..56253
                     /locus_tag="PKI35_00210"
     CDS             54478..56253
                     /locus_tag="PKI35_00210"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HNX42829.1"
                     /translation="MNRLKGSFPLVGIRPVIDGRRKGVRESLEEQTMNMARHAAELIS
                     STLHYPDGSPVKCIIADTCIGGVAEAAECAEKFARNGAGVSITVTPCWCYGTEVMDTD
                     PLIPKAVWGFNGTERPGAVYLTAALAGYAQKGLPAFGIYGQDVQDADDQSIPADVREK
                     IIRFTRAALAAAYMKGKSYLSLGSAAMGIAGSIVDPSFFSDYLGMRNEYVDMSEFIRR
                     IEEGIYDHNEFEKALDWAKANCLEGPDMNARPFPQDQKEKTWEFVVKMTLIARDLMIG
                     NPKLVRLGFGEEALGHNAILAGFQGQRQWTDHFPNGDFMEAILNSSFDWNGIRQVFLM
                     ATENDAFNGVAMLFGHLLSNTAQMFSDVRTYWSPEAVKRVTGKSLEGQAANGIIHLIN
                     SGASALDFTGQQTIDGKAAIKPFWKITEEEANRCLKNTRWPSAIYEYFRGGGFSSEFS
                     SRGGMPVTMSRINLIKGQGPVLQLAEGWTVELPADVHQILTRRTDPTWPTTWFAPRLT
                     GSGAFTDVYSVMANWGANHGAISFGHIGADLIALASILRIPVCMHNIKASAIFRPAAW
                     NAFGTADPEGADYRACKNFGPLYGR"
     gene            56277..57758
                     /gene="fucK"
                     /locus_tag="PKI35_00215"
     CDS             56277..57758
                     /gene="fucK"
                     /locus_tag="PKI35_00215"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: protein motif:HMM:TIGR02628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HNX42830.1"
                     /translation="MDKAVLVLDCGATNVRAVAVGANGSLLASRSLPNMVRPDPDFEG
                     GLIWDVEEIWQKLCACSRHVISSLQDTAIGAVTATTFGVDGAPMLKNGTLCHPVISWQ
                     CSRTVPVMNAIGKYIDPAGLSAITGLQPYHYNTINKLVWFRENRWDVLRVMKSYVMMP
                     SIFLHRMTGEFVTDTTMAGTTMLTNVKSRKFSDIILEAIGIGTKIFPPLVEPGTVVGK
                     IRASAAKDLGIDPGIPVVATGHDTQFAVFGSGAGINEPVLSSGTWEILMSRTPAESIN
                     IPPVSEGVTCEFDAVPGLIDLGVQWVASGVLEWITALFYGEYKDSPSRYQLMIEEAQA
                     IPAGCCGVTMLPELFDGGFIPSKGCINGLRHDTSRAQIYRAALEALAYYTRTGLEILK
                     KAGAFTPGSLLGVGGGTKNRLWNQIRADVLQMPFKVSNRSETTVLGAALFAMPAAGLY
                     HSVEEAVLSVENRVETFLPGAGTGAYQQLYSDFRANWLPESNL"
     gene            57775..58923
                     /locus_tag="PKI35_00220"
     CDS             57775..58923
                     /locus_tag="PKI35_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNX42831.1"
                     /translation="MKNKKVFVTGCFDMLHSGHVAFLKEASGYGELYVCIGSDNTIHN
                     LKGRYTVNSQDERRYMIEALKCVKECRINSGSGIIDFENELHDIRPDIFIVNEDGHTP
                     AKEALCSELGIEYRVLKRIPYADLPVRSTTALRNVCRIPYRIDLAGGWLDQPFVSKYH
                     PGAVLTISIEPTVEFNNRSGMASSTRRKAIELWRTEIPHGDLDQLARMLFSFENPPGT
                     IEIAGSQDSLGIVYPGLNRHYYESNYWPVSTESIQDEEILSWLENHLYLVTLGPRVSE
                     YTVIDNTNISTDGAKSLAAAADACWDAILRKDVNAFGSAFRQSFEAQVAMFPNMAGRE
                     IFEIIDQYKHLAKGWKLSGAGGGGYLIMVADRPVDDAMQIRIRRQNGL"
     gene            58975..60219
                     /gene="fucP"
                     /locus_tag="PKI35_00225"
     CDS             58975..60219
                     /gene="fucP"
                     /locus_tag="PKI35_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HNX42832.1"
                     /translation="MKHKLTTKDFILPFIMITSLFFLWGIAHGMVDTLDKHFQQILHL
                     KKWQSSFIQFSLYGAYFTMALPAGYFMRKFGYKKGIIAGLSLFAAGAFLIAGTATFES
                     FWLFLICLFILGCGLATLETAANPYTTKLGPPESAEQRINFSQSFNGLAWVLGPLIAL
                     SIYGNQSEAEGEKMLSLIIPMGIIGLVVLLFALIFMRLPLPEISEEDASIAHGTHVSG
                     VEEKPSFFIPLSKQPHFIFGVIAQFCYVAAQTGIFSYLINFVTDEGQYPQFDVKYAPY
                     FLSIGFALFMLGRMAGSYLMGITKPTRILAIHAALCCLLLPVVSSHLGWISLISLYAI
                     FFLMSIMFPTIFALGIKDLGPKTKKASSFLVMAIVGGAIFPPSMGYVSDLYGMSTGFL
                     VPIPLFAFILFYALKGHQVKAC"
     gene            60281..61726
                     /locus_tag="PKI35_00230"
     CDS             60281..61726
                     /locus_tag="PKI35_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX42833.1"
                     /translation="MKRALMIIILFAVAAGYLPAQEHESPYIPPADPLVLKNLEEWQD
                     MKFGIFMHWGTYSQWGIVESWSLCPEDEGWTQRKGEFSDNYFRYKMAYENLQTSFNPV
                     DFNPEKWAAAAKEAGFKYVVFTFKHHDGFCMFDTRLTDYKITSPKAPFHSNPKANVGL
                     EVCKAFRNEGFKVGAYFSKPDWHCPDYWWPYFPPKDRNVNYDPARYPDRWNAFKEFTY
                     KQIEEILTGYGKIDILWLDGGWVRPMSTVDKNVEWQRGIVYDQDIDMASIAKMGRKNQ
                     PGLIVVDRSVGTEFENYRTPEQQIPDKPLGYPWETCMTMGNSWSYVPGDTYKSVWTIV
                     HNLVDIVAKGGNYLLNIGPSPLGDYDPVAYDRLKGIGEWMKVNGDAIYATRPVAPYKE
                     GNICFTRTKNGAVNAIYLAAENEKILPETIVIKGITPKKKTDITLLGAKGNLKWKTVN
                     GITTIAIPDKLRKNPPCRYAWTFRISEVDQK"
     gene            complement(61955..62734)
                     /locus_tag="PKI35_00235"
     CDS             complement(61955..62734)
                     /locus_tag="PKI35_00235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNX42834.1"
                     /translation="MKKTTVFILMLAILAGGTSAFAQKKDKKSRKAKEEAAVTLPQMK
                     NETDTVSYIIGTDIIRSFKKNNIEINYDMLNLGIKDATNNADSLFTQEQIMQIFQSWN
                     QKMMKARQDREAAELTQTLEANAKFFAENKTKPGVVETPSGLQYAIIKPGEGEPPTDT
                     DIVEVHYVGKLLDGTVFDSSRDRGQPAKFPVNGVIPGWSEGVKLMKPGAVYMLYIPAD
                     LGYGNQKQGPIPAGSALIFEVELLSVEKGDVNKEEQEQGKE"
     gene            complement(62927..64768)
                     /locus_tag="PKI35_00240"
     CDS             complement(62927..64768)
                     /locus_tag="PKI35_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX42835.1"
                     /translation="MKHLFTLFAFCAIAPAFAQQKMPVIPENACQKVFKAKPVLANGE
                     NNPFTSSENRYVNAFSQYACDTIGHTRYDLQSNCSAPSGRLTLFPDETMATVWTRGID
                     ASGSDRGTGYNYYYTYDWGYNIDPERLEDQRAGWPSHARLGTNGEIVVSHKSGSPAPL
                     FMLKREIKGTGDWMQSTIPLPENAPGLLWPRMVTGGSDKNQVHIISLTTPVDNSGALY
                     HGQDGALIYSRSLDGGISWDRNEVIPGLDSAFYPGWTADRYCLAEPRYNTLAFAVSNA
                     ADDLILMKSGDNGDTWQKTVVWHYPGQAGDVIYKPDGAVSVVIDFCNKVHLFFGVVLS
                     NAAGDLFPYQSGIAYWNEDMPVWEGDDQYLAGCLNPDTLSNQNLLVASPVDVDGNGQW
                     DVLGDCGDYNVGAVSMPQAKVDGSGFGILAWSAVTENCNYMGMDYRHIWVIKTTGNFS
                     EFSSPVDITAPFDPDNNAECVYPGVCQQPSLSCPASYYLIYQRDQLPGLQSPTDPSEN
                     YIICSSELGYVPGTGEPAEAKLLVSQNRPNPFTGKTQAEITLTKPASVSLEVTTLTGC
                     SKWNKDYGTASAGVHTLIIDATGLAAGVYFYTVTAGSRKMTMKMMVL"
     gene            complement(64837..66969)
                     /locus_tag="PKI35_00245"
     CDS             complement(64837..66969)
                     /locus_tag="PKI35_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX42836.1"
                     /translation="MKPLIISALIGLSLFAATFCADAQEWRWRWAAQNYGYSKYNLSS
                     ILTTDLENNIFTGITYCDSLFIDDTVFFHPGYEGYTRHQLIAKYDRKGDCKGYIDLRG
                     PILDGLWSVTAVTDAEGYVYVGGDFCDSLYIEDTTILPLPHQGFVFSNVFLAKFSPDL
                     RLQWVKLIGGDTQNRLLGLKANGNSIYLTCEHMANGNMPFNAYILGQDTVHSIKPVCS
                     MMQVDTGANISWITHIENSGFNLRNFLQGGDGRLYVTGHTNYYPANIIVGNDTIQCPA
                     NSMYHSLPFLIGLNTDGSFYKGWIKDWDVYTLQTVVDEDGAFIFTANISDSITVGGVT
                     VTNSGDSIASAVVKINNEQEPEWIRTFRSPHIQINHDLLITLSNNQLFFGISFQNRLK
                     TGDTTFHIDYWYENLVGRLEDNGMLAGTILSNTDYHSRMAGIGTDNCGNIIITGWFIG
                     NGIYGNDTITSRQSWSTNDLFIGYLDLNEYAFDLGPDTISCHDYTISGPAGWVRYYWN
                     GTLTPGKDFYTTQSGMYRLTVVSAGNCWQSDSVHVTIQQPPVFNIGPDTTIKRSHILT
                     LSMPEGFGRYLWSNGDTTSTITFPGNHFASGQYTIWGEVTEELCSVRDSMNLSVINDI
                     GINESAGTGFMLYPNPATSVLGVEVSTDFSSDEGVLTILNATGKMQLECYLNGNKKQI
                     DISTLPPGLYFVRLSNEKTVEVGKFVKE"
     gene            67350..67907
                     /locus_tag="PKI35_00250"
     CDS             67350..67907
                     /locus_tag="PKI35_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX42837.1"
                     /translation="MNSITDFQYDLIPTLFTKRLILRPFALTDGPTVRELAGNIKIAE
                     NTLNMPYPYPEGEAEKWIATHAAEFSGKRNLVLAICKKDDSTLVGCIGLQLNLYVGNA
                     ELGYWIGEPYWKNGYATEAAREMIRFGFEKLGLHKVFAHVYVTNPASAKVLRKAGMRQ
                     EGYLEKHVRHWGNYKDLLIFGIASV"
     gene            67992..>70936
                     /locus_tag="PKI35_00255"
     CDS             67992..>70936
                     /locus_tag="PKI35_00255"
                     /inference="COORDINATES: protein motif:HMM:NF037694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNX42838.1"
                     /translation="MKKPLYFLLLLAITLASCTNLKTVRVKKTNFDPETQRGQNLVFT
                     FSHELVSDSGLMNKWDTTTYIKFEPKIPGKFMWTGKSELTFSPSQLLAPSTDFKATLT
                     NAILKYDKKKLSVDDEALPFHTPYISLESVSSYWALGSDPAANIEVRLLLSFNGPVSA
                     ASLASLATLEVKGKSRQFRVITQNDSDVIELAFADETQSPDEVTEGKITIGKGFSCSG
                     SNYKTTDAIVMTFTVPSKDKLTITDISSDFEEGEGVISIFTTQPVIDEGLSSLISVQP
                     AVSFTTEILPNGVKLKGGFLANEAYTVKVSPALKSVFGRELGEEFIQTVAFGSPEPYV
                     SFTEKHALYLSTRGSRNLGVNIINIPEVRVSVFKVYENNIQHYTKLGPEWNWQYDENN
                     DEYHEYMTYAFDEDYGRQVMSKVIKTRSLPKSGNISLLNIDLADFNVTDQFRGMYVVK
                     VESADRRWLQDAVMVSLSDLGMIVKEGTDNVLVFVNSLFNASPVKGARVDFYSSNNQK
                     IYSATTDSRGTALFKNIKSQAPGFRLSLVTANYQEDFNFILFNSTRVETSRFDAGGKR
                     TGNLNYDVFLYGDRDLYRPGDTVYYNAIVRDLGWQTVRDLPLKIRILAPNGKEYQSQR
                     KQLSNNGAAEGSFYIPVAAMTGTYSIEVFSSNNILLASRRVAVEEFMPDRIKVAVSTA
                     KNAFDSGESIQVDLQADNLYGTPAAGRKFETELRLSRKNLKPKAFPDYNFTVETKNMP
                     YLATITNTGTTDASGKASHLIKPGVYEGIGILDGTLFTTVFDETGRPVNRLNKIEVLT
                     QPVFLGIKNFDQWVGTRKPMNFKVIAVNKDGKAQGNVQAQVVVSHLRYETVIERDGDR
                     YRYVSQEKESVEYSKTVAVPSTGAVIPFTPAQSGKYVLKVMLPGSQNYVSSTFYAYGW
                     NDTDFTSFEVNREGEVTISADKESYQPGNKAKLLFSAPFDGKMLVTFSRDNVLEFKYI
                     EVKNKAASLV"
BASE COUNT        18843 a        17692 c        16297 g        18104 t
ORIGIN      
        1 tgcaggatgt tcaccaggaa gtcggtaaat tcgaaaatgt tctttttcaa ttggtgatat
       61 tcgttgtgaa gcgccgaatt gtagaacatt tcatcataat agttccttac gaatcctacg
      121 cacgaagccg acggactgac gatataacgg ttgttggaga aatcgcggat aaacttctcc
      181 cccactgctt tggcttcatc ccaataaccc gcattgaatg ccgcctgtcc gcaacaggtc
      241 tgtacagggt tataatgaac ctttaccccg aatttttcga gtatcttcac catgttgaaa
      301 ccggtttcag gataaagctg gtcgataaaa cagggaacaa aaatatctac atcaaggggt
      361 gaaagcatgt ctgacaattt aaatgctggc tctgaaacag aatgccccaa aacgttccgg
      421 gcacatttct gtaaaaacaa aaatatacaa atagagcccc tgctgaaaat gaagttttca
      481 accggatgtt aataaaaagg gctgtcatct attcccaaca gaaattttca aaccggaaac
      541 ctgctgattt caggtcattc cagaaaccgg gataagactt ccgcacttcc gccgggttat
      601 cgatcccgat tttcccggtt ttaaacgcca gcggggcaaa agccatcgcc atgcggtggt
      661 cgttatacgt atggatgacg attttgtcgt cccctgagac cggctggttt ccggcaatta
      721 ccagatcgga acctttttcg gcatgaacgc gatacccggt tttcgtcagt tccgaaacca
      781 gcgccgacaa cctgtccgtt tccttgatcc gcaaaccgga taatcccgtc agccgggccg
      841 ggatattcag acctgcgcag gttaccgcca ctgcctgagc cagatcgggg caggaggtga
      901 aatcctgaat aaaagattcg tcagaccgga tgcggtactt tttcaacagg atgccttccg
      961 cagtttcggt ggtacctaca cccagttttt caaatattcc ggcggctatc gcatcgccct
     1021 ggagactatt aaaactaagc ccgggcagga tgacttcagt ctcatccgaa agggcaacaa
     1081 cttcgtacca gaaagcggct gaactccagt cggcttccac gctcaagcca tcaaccgggg
     1141 gcgtataggg ttttcccgca acacggacca gatcggacgc cacggtcaca tcagcgccgc
     1201 attgctgcat gatccctgct gtcatggcga tatacggggc tgaaacggct ctgcctgtaa
     1261 tacggatatt caacccgccg ggcaacgacg gggctatcag catcaaggcg ctgataaact
     1321 ggctgctgac atcgccgggc gctgatacag aatttcctcc tttaatcgcg cccccccgta
     1381 ttgcgagcgg cgggaattct ttttcgcctg tataccggat atcggcgccc gattccatga
     1441 gcgcatctgc cagcacacct accggacgtt gtttcatccg gccggaaccc gtcagaaacc
     1501 aattgccggg agtaacggcc agcagggccg tcaggaacct gaagacggtt cctgcatctc
     1561 ggcaatcaat cgtttggggg gtccggcatc gggccgatgc attcaccatg gccagcaatt
     1621 cctttaacaa aaccgtatcg ccggcgtccg aaaggtttct gatcgcgagt gtaccacctg
     1681 ataactggcg tatgatcaac aggcggttgc tgatgctttt ggaagcgggc aaatgcactg
     1741 aggttctgac caatggcgtc gccggaggaa taacgatcat gccagaaagg aatggtttat
     1801 atcgctggta aaggtaacga aaatgaaagg attaatcttc agcagtttaa cggctgttca
     1861 ttattgacgc cgcaggcagg gtatattacg aagttggtga tcagggggaa gagaggtttc
     1921 agaatttgaa aatttgaaaa tgtataaatt tgaaaatttc agtttctgat taccggtttc
     1981 tttcggacgt tggctttagc tgttggctct tggcgttctg agttctgagt tctgaatttt
     2041 gtgttttgcg ttttgcgttc agcttttggc ctgccgtctt acccacaacc ccgcccaggc
     2101 aggtaaactt ttagccgttg gtgttttgag ttgtgtgatt cgttgttcgt gattcgtgtt
     2161 ttgcgttttg cgttttgcgt tttgcgtttc gtgttctgcg ttctcccttc tccgtcctcc
     2221 gttttgcgtt ctccgttctt cctcctcccc tgcctgccac ccgcaggctt acgcaaggca
     2281 ggcaggcctg cccgctcact cacgtccttc gctggcaggt aaagttctcc ctgccccctg
     2341 cctcttgctc cttgctcctt gctccatgcc cattaccgga gaagtgtaaa aaggaattca
     2401 ggctgtcttt acagattcac gtaatagtgc agactttcgc gaacgaggct ttcgctggga
     2461 tactggtcaa cgaggggggc tccggggctt cccagcaggg taaaattgat ttcgccttcc
     2521 tgtttcacct ttttatcata cagcatgatt tcgatcagtt cgttgccggc aggagcgtaa
     2581 aaaatggttt tacggaaatg ttccatcacc agctccacaa tttcataaag gacggagatg
     2641 ggcaacccgc aatgtttcac cgagataaag gcttcgcata tcaagccagc cgccacggct
     2701 tctccgtgga gcatgggttc ggacatatgc atggccgacc atgtttcgaa tgcgtgcccg
     2761 atggtatgac cgaaattcag cacattgcga atccctttat ccaacgggtc ctgttttacg
     2821 accctcatct taataagcgc tgactgcttt atggccgatg gcgctgacac gatttccgtc
     2881 ggggatatct ttttcaggtc ttcaaaactt tcgccatcaa tcagggcgtg tttgatcatt
     2941 tccgaaaagc cggaaagtat atgccgggca ggaagtgttt taaggaaatc gggaaataca
     3001 caaatagatt ccggctggct gaaagtaccg atctggtttt tcaaaccagc caggttgatt
     3061 ccgtttttac cgccgatggc agcgtccacc atcgccataa gggtagtggg aaccatgacg
     3121 aacggcattc cccggcgata ggatgaagca gtaaatccac ccacatcaca aaggacgccc
     3181 ccgcctaaac agaaaagcaa tgaatcgcgc aaggcccctt tttccgagag ttgttcccac
     3241 accgaaactg ccgtatggaa atttttagct ttttcacctt cgggaatttc aacaatcctg
     3301 gcattggaca aggaaggatg gcgttcggcc agttggggat aacaatactt tttggtattc
     3361 tcatcaacaa gaataaatat gccggccttt gcatgcctgt tcaccacctc atcaatggca
     3421 gaagaagcct ccggaccgaa atatggagct acaatatcct gcattccaaa gaattatttc
     3481 tcaatagcat tactaaccac agaacgtgat aattaaccaa gacctcaccc taaccctcac
     3541 aatcagacct caccccaacc cctctcctaa aggagagggg ctctcctccg cgatggccct
     3601 gcatcccctt cctgcaaatc ctttaactaa aaccttgatt tatcagccgt tcactattgt
     3661 ttcaaaagta caaaaagtgt ttatttcaat ggtataaaaa tttataaatt tttattaccg
     3721 ataaattatc ggcttattat gcggccaaac gaaactgttt cccggatgca gaacctgcaa
     3781 attgtcgtat ctttggaccg tgatccgaaa cacatccata cagcccggcc attattgcct
     3841 ttcgctctac catcatacag ggcgcggcct ttatgagtgc tggatgtgct gatcccttcc
     3901 gttttccttt ctcttttcct catagcgaat aaatttcgcc tttcaccggc ttatctttat
     3961 cgttagggga tataagcatc ttgtcaggaa ttatttttac cataaaacag gaagagaaaa
     4021 acaatgaaag ggttgatttt cgacatcaaa cgatactgtg ttcacgacgg gccggggatc
     4081 cggacgactg ttttcctgaa aggctgcccg ctcaactgcc tttggtgcca caatcccgaa
     4141 agccgcctgc acgaaataca atacgtacac cgcaaacgga atatcggcaa ccaggagttt
     4201 gaagaagagg agatcatcgg gcgatggtac actgtggccg aagtcatgaa agagcttgag
     4261 aaagacagcc ttttctatga tgaatccggc ggcgggatca cgctttcggg aggcgagccg
     4321 ctgatgcagg caggtttcac cggggaactg gcctccgcct gccgccaacg cggatggcac
     4381 gtagcccttg acacttgcgg gtatgcatcc cgcagctctt tcgaaaaggt ggtgccttat
     4441 atcaacctgt acctttatga cctgaaagga atgaacaacg ccgaccacct gatccatacc
     4501 ggcgttgaaa acagcctgat actcgacaac ctcatttctc tgctcgatgg tgggggcaag
     4561 gtcatttttc gatacccggt gatccccggc atgaacgaca gcgaaaacca cctgctcgat
     4621 ctgatgcatt tcctgatgaa cctcaggtca acccagcgtg aaatccacct cctgccctat
     4681 cataccattc agaaaagcaa gtacgaaagg cttgggctaa cttaccggtt aagcgaactt
     4741 ccggcagcca ccgatgaatc gctcagggag atgaaggcag ggctggaatc cataggctat
     4801 accgtcagga ttggcggtta acccaatccg gaaatcattt tattcatcca gtaacaaccc
     4861 gagaatcaaa aaacataatt cgccatgatg accgaccgca ttaaaaaact tcgtcaacaa
     4921 agccttgatg ccgttaaccg gctcactccg gagcgcgccc tgctggttac cgaattttac
     4981 cgcgacaacc gtgaattttc aatcccagtg caacgcgcac tctgctttaa atacatcctt
     5041 acccataaaa gtatctgcat caacgatggc gaactcattg cgggtgagcg cggcccggag
     5101 cccaaggcca ctcctactta ccccgaggtc aacctgcatt cgatgcatga cctggaggta
     5161 ttgaattcac ggccaaaggt ttcgtttaaa gttgacgacg agatgcggtt ggcatatgaa
     5221 aacgtggtca tccctttctg gaaagggaaa agccaccgcg aacggatgtt cgccgaaatg
     5281 actcctgagt ggatcagagc ctatgatgcc ggaatgttca ccgagttcca ggagcagcgc
     5341 gcgccggggc ataccgtcct tggtaaaaag atttacagca tgggaatgct ggacatcatc
     5401 ggtgaaacag accgggcata cgcaatgctg gacattgaga acgacaggga tatcctgccg
     5461 aagaaagaag aactgaaagc catgcgcatt gcggccgaag cgctcatcct gttcgccgaa
     5521 cgccatgccg cggaactgga caacctggcg gcttcggaaa ccgacgccgg ccgcaggcag
     5581 gaacttataa acatggcgac catctgccgc aacgtaccgg cccacgcccc gcaaacattt
     5641 catgaagcgc tgcaatatta ctggttcgtg catctgggag tcatcaccga gctgaatccc
     5701 tgggattcct tcaatcccgg acgtcttgac cagcatttat acccctttta taaacgcgaa
     5761 accgaagccg gaacgcttac ccgcgaaacc gctgaagagt tgctccagtc gttctggatt
     5821 aaattcaaca accatcctgc ccctcccaaa gtaggcgtta ccgccctcga aagcaacaca
     5881 tacaccgatt ttgccctgat caacctgggc ggcgtgcaga gcgacggttc ggatgccgtg
     5941 aacgaactca cgtttctcat tctcgacgtg atcgaagaga tgagaatact gcaacccagc
     6001 tccatgatac agttgagtaa gaagaacccc gacagttttc tccaccgcgc cctcaggatt
     6061 gtaaaaaccg gtttcggcca gccttccatc ttcaataccg atgccatcgt gcaggaactg
     6121 ctgcgacagg gaaaaagcgt tgaagacgcc cggaacggag gcgccagcgg ctgtgtggaa
     6181 accggtgcat tcggcaccga agcctacata ctcaccggct atttcaacct taccaaagtc
     6241 cttgaactca cgctgaataa cggccatgat gcacaaaccg gcgaactgat tggcctgcag
     6301 accggtgaag cttcctcatt caactcgttt gacgaactga tgtcggctta ccggaagcaa
     6361 cttcgttatt tcatcgatat aaaaatcaag ggtgaaaaca tcatcgagca gctatatgcc
     6421 cgttacctgc cggttccgtt cctgagcctg ctcattgacg attgcatcaa aaacgggcgc
     6481 gattataccg ccggcggagc acggtacaac acttcctata ttcagggcgt cgggctggga
     6541 agccttaccg attgccttac cgcgctgaaa taccatgtat tcgacaacca taagatctca
     6601 ttggcggaga tgttacaggc cttgtcggaa aactttaccg gtcatgataa actgcgttat
     6661 gacctggtat ataatacccc gaaatggggg aacgacgacg attacgccga cgataacgcc
     6721 cggatgattt tcgagatttt ttatgacgcc gtgaatggac gtccgtcgcc aagaggcgcg
     6781 acctaccgca ttaatatgct tcctaccaca tgccatgtat atttcggcag caagatcaac
     6841 gccacccccg acgggcggct ggccggtgag ccgttgtcgg aaggcatttc gcctgttcag
     6901 ggcgctgata aatccggccc gacagccgta ctcaaatcgg catctaaaat cgaccatatc
     6961 cgcaccgggg gaacgctgtt aaaccagaaa ttcaccccgt cgtttttcga cagcgaagaa
     7021 agtattgcca aactcggaaa actggtaaga acttatttcc gcatggacgg ccatcatatc
     7081 cagtttaacg tggtgtcggc agccacgctg aaagacgcac agaaacaccc ggaaaaatac
     7141 cgcgacctga ttgtacgcgt tgccgggtac agcgactatt tcaacgacct gggcgaagac
     7201 cttcagaatg agatcatccg ccgtacggag catgaaagcg cctgatgatt ccgatagata
     7261 atacagaagt agtttttacg gaattattca cctgtgccgg aaatgtgtaa tttcgggttg
     7321 tcaagttttg ccctaacctt aacctgaatg tcgtcatacg tcacccgatc gctgttaagc
     7381 gtattgtttt ttatatcctt gtccgtcaac ggacagaatg cccttatccg taagattttc
     7441 cccgattccg catataactg ccgcgatctg caatatcaaa gcatgcagct tattcctttg
     7501 tattacggga accgcgattt cgatacggcc aatgccatcc tggattactg ggcgggacaa
     7561 tgctccacgg acgaaactat tttcaggacg cgcatattgt ttgccatcga caacggcact
     7621 ttcagcgatt cgttgtttaa aaatgacgga ttcacttact accttgatgt ttacaaagag
     7681 ctggccaatg acacttccgg cagttccctg tactactacc cgtattatta tgtggatgaa
     7741 atagaattgc tgaaatggta caaaacgttt accgacagca ttgctttccg ttgcctgacc
     7801 tataccgacc tttcgcccga agaacgtttt tttgccacct attatgccta tccatccgac
     7861 agcatctata cgctgctttt tgctaaagta tatgacagca cggccttaaa aaaaattgcg
     7921 tcggaacccg tgtacggtca aatggaaaat acccagggcc attattcctt cagcaccggt
     7981 atgtggattc ccgaccagcg ccttgataca ctgggccgtc acccgtatct gggtgcgcaa
     8041 atcgggttga aacacaaccg gtacctggcc gacctggtgc tggacctgcg tttcggaagt
     8101 tcgcccaaca cctaccaggt tgtcattgat ggtgaaacct gggataccga aaagtttttc
     8161 gaaatgagct ggcgcctcga tttcgggtat gaattgttta atatccggag gacagaaatg
     8221 ctctttctgg gagggctggg ctacgactgc atcaacgcgg ttaatgaaac caacgatccc
     8281 gacaatgaag acgacgacgt gagtaaatac ctgcattcgt tcaacatgaa ccttggcgga
     8341 agttaccgtt actacctgaa taacaaccat tacctggctg tgaccgggaa ataccacgtc
     8401 ctcaatttcc gcaacaaagg cggcaccgac ctgaaaggca atgctttaac attatccctt
     8461 gagtatggtt ttggcatgaa ccgctggctc aacgacagga atacgatact gagaaagaaa
     8521 acaaaaaatt accgtcagca ggtcaggcaa agcctgtaat gctggtccgg aggaatcatg
     8581 caatatttat cggaataatc cgatgagtaa caggacgact ctatttgcct accgtcttcc
     8641 agattttccc atcctgggta atatgccaca gcgtgccggc ggcgctcccg ttggcaaccg
     8701 cctcatagag ggatttggta aggctgattt caggcctgaa aggtttcccg ttgacctgat
     8761 aatccgaaaa ccctaacgat ttaaggttgg ccgtatagga accatttttc ccgaaccatg
     8821 ctttttccct gaaatatagt ttccgcaacg cctgcatgat ggaataatcg ggattttcaa
     8881 ggtagatgtc ggttccgctt ccggcggtct tagccgaaaa ttgcaggaat ccccaggttt
     8941 caggctggtg catggctatt acaccctgcg gcgaccaaac ccagttatct tccggtaaag
     9001 gcttttcttt tccatcgatg aggcgcgtgc gcttcacgta gcggccattc acaatatcca
     9061 tggtccattc aacgcgggag aaattgatcc ttatctggtc gccggcaccc ggacgtttcc
     9121 ccccggtgtt cacctccgag attgcatcga gaggcagcgc cacttccaca taccagccgc
     9181 tgtcggtatc gcagggattg ttgatagtgc catcaacaaa cgcccctgat ttcatcccgt
     9241 tataattcca gttatcaacg gccacattcc acaccacgtc gtcgcggtaa ggtttcgtga
     9301 tcagcaaatc ccattgcgta ttaaaggcat tcatctcata ttcaaggtag ttatgggtgt
     9361 cgccatccgg atcgatgaaa atttcaaaat ccgggtcata aaaaatgacc gattcccttt
     9421 cggtgagtgt tgcccaaaca tgcggttcct ccaggaatgc agcaacatat aaataattat
     9481 catcccaaag cattttggcc ctggttttaa gccgcggttc cggtttcgcc gaaccttcga
     9541 tatccccgaa atattctgtc cagggcgctt tgccccagtc ggcttcgttt aactttccgt
     9601 caatattcag gggagcagaa gtcctgtaac acacataatg cctgggctga acgggaacga
     9661 gaggctcctg cggccttgcc tttaacagca ggaaggcaga aaaaacaaaa atcagaagaa
     9721 aggtgcggtt cataccttga ggttttaagt tgtttacgat atacaggatt atatcagaat
     9781 gccggtccat aggttatccc cgagcccgga ttccggactg agaaattgaa ggcaggagcc
     9841 ggcagccaac aatcacaccg gaaatgccgt cagcaatgcc gctgatttcc gcaatcctgc
     9901 cactgccccg ttatccacca acgggaaacc ggaccttctt cctgatgatt ccgcggcaaa
     9961 ataacaaaat tataccgatg gaacattcgt aatcgtccga tcgcctttga gtgaacggct
    10021 aaagactaaa agcaacagac aaaacggagg acggagaacg gaaaaagaag gacgaataat
    10081 gagccacgaa ttccacgaat gcaatcacga atttcacgaa atgatgacaa caaaatacaa
    10141 aacacaaaat gcagaacgca aaacgcagaa agcagaactc acaacacagg actcagaact
    10201 caaacggccc gatgaagaac tatgaacgca aaacacaaaa cataaaacgc ccgaatcacg
    10261 aacaacgaaa cacgaaacac aaaatccagc taccagatca aatattactt ttccttcagc
    10321 ttctgaaaac cagcagctta tatctcacgg caccggcggc tttacaataa ttccttttat
    10381 ttgccgttca ggggagttaa agactttttg cctgcctact ttttgataaa atttataaag
    10441 cttaccttta cgccttgaat cactgaatta tacttaacct aatcatcata aaatgaagaa
    10501 ttctgttgca ggcctggctt cgatcctggc tctcattttg ttgagtttca ccggcttcgc
    10561 tcaaaaggcc ccggtagatt atgtaaaccc gtttatcggc acaggcggtc atgggcatac
    10621 ctatcccggc gctacaatgc ctttcgggat ggttcaatta agtcctgata cgcgcctgga
    10681 aggctgggat ggatgcggcg gctatcatta tgacgatgaa tatgtttacg gattcagcca
    10741 cacgcacctc agcgggaccg gcgttcccga cctgtgtgat atcctgatca tgcctacaac
    10801 gggaaaagcc cggtttaata acggctatga cgggaagccc ggttaccgtt ctcatttcag
    10861 ccataaaaac gaaaccgcca caccgggata ctacaaagta cagctcgacg attatggcat
    10921 cactgccgaa ctcactgcta ccgaaagagc aggtttccac aaatatacct tcccggccag
    10981 cgacagcgcc aacatcctaa tcgacctttt ccatcgcgac gatgccaaag aagcttccat
    11041 ccaggtaaaa ggcaataacg aagtggaagg cgtacgttac tccgaaggct gggcaaataa
    11101 tcaggttgtg tattttgtcg cacagttttc aaagccgttt tcgggagtca gcctggcctc
    11161 gaacaatgtt gtattgcccg aaaggctggc gcttgataaa cgtaagaatg tgaaagcagc
    11221 ctttcatttt aaaacggccg aaggtgaaac gatttacgtg aaagtcggga tatctgccgt
    11281 gagcatcgcc ggcgcccgta aaaacctcga aaaagaaatc cccggctggg atttcgacca
    11341 ggtacgcagc aacgcccgcc aggcatggaa caaggaatta tcgaaaatta ttgttgaagg
    11401 aggaaccgat gatcagaaag cagtatttta tacctccctt tatcacacca tggttgtccc
    11461 caataccttc atggatgttg acggcatata ccgcggccgt gattttaaga accacaaagc
    11521 agaaggattc accaattatt cggttttctc actctgggat acttatcgcg cctatcatcc
    11581 cctcatgacc atcatcgatc agaaacgcac cctcgactac atccgcactt tccttaccca
    11641 atacgaccag ggaggattat tgccggtatg ggaacttgac gccaacgaaa catggtgcat
    11701 gatcgggtat cattccatcc cggttatcgt ggatgcatat atgaaaggca ttaaggactt
    11761 tgatgtcaat aaagcgtttg aagccatgaa aaaatcggcg acgcgcgaca accccggtct
    11821 gaaagcgctg gatcagtatg gctttattcc cagcgaccgc gaagccgaaa gcgtttctaa
    11881 aaccctggaa tatgcctatg atgactggtg catcgcacag tttgccagga gtatcggaaa
    11941 aaaggacgaa tacaagtatt tcattcaccg ggcacagaac tataagaacc tgtacgatcc
    12001 ctcaacaggg aatatgaggg cccgtatcaa cggtggctgg ttcacgcctt tcgatcctac
    12061 agaagtaaac tacaacttca ccgaagccaa ttcatggcaa tacagtttca cagcaacaca
    12121 ggatatcaca ggcctgtcga atttgatggg cggtaaagat aaactggcgg ccaaactcga
    12181 tgcccttttc agcgcatcaa cgcaaaccac gggccgcgac cagagcgaca tcaccggtct
    12241 gatcggtcaa tatgcccatg gcaacgaacc cagccaccat attgcttacc tctacccgtt
    12301 tgccgggcaa ccctggaaaa cacaggaact ggtgcaccgc atcatgaacg agttttataa
    12361 aaacaatccc gacgggctga ttggcaacga agactgcgga caaatgtcgg catgggctgt
    12421 attaagcgcc atgggtatct atgccgttaa ccccggcagt aacttctatg tgatcggcac
    12481 cccctggttc gataaagtga ccatcaacct ggaaaacggt aaacaattcg tcatcaaggc
    12541 aaacaacatc tcaccggcaa attattacat ccaatcggcc accctgaacg gtaaaaaaca
    12601 caatgcatcg tacctgaagc atgccgacat catgaaggga ggagaactgg tattcaccat
    12661 gggaagccag cccaacacag aatggggcgt tggtaaaaag aacgaacccg ttacagccat
    12721 caccgacaac cttgtactac cggccccata cgtgaaagat gcagtgaaaa cattctccgg
    12781 tgctaaaact attgaactgg ccgggccggc cggcgccacg gtaatggtta tgacctccgg
    12841 agccgaccgt atcggtgaga acacccctta tactgctcct attgaactca ctgaaacctc
    12901 gaaagtgaaa tttttcgccc agcagaaaaa acttgtcagc cctacggtga cagccgaatt
    12961 tattaaatcg gaaatcaaca aggattgcaa actcatcacg gcaccggcac aacaatattc
    13021 cggaaacggc gggttaaccc ttgtcgacaa catcaggggc aatcaggact ttcgcgttgg
    13081 cggatggctg ggttttgaaa aagaaaactt tgaagctgtc gttgatctgc gcgaaagcaa
    13141 agaagtatca aggctttcag ccggtttcct tcaggatgag aattcctggg ttttcatgcc
    13201 gacgcaggtt gaatttttcc tttcggacga tggcgtcaac tataaaagcg ccggcgtcgt
    13261 caagaatgat gttccctggc aaaaaacagg cgcctttacc agggatttca acctgaacat
    13321 cactgctgcc aaagcccgtt acgtcaaagt aatcggaacc aaccggggaa cctgtcccga
    13381 agggcacaaa gggaatcccg gcgctgcctg gatatttgtc gacgaaataa ccgttgaata
    13441 acccggacca aaataattaa agcctgccgg agaatatccc ggcaggcttt tttatttgaa
    13501 actcctcttt tcatcataac tttgttttct gttgaacgac aaacaacagt cacaccccct
    13561 ctaatccaac catcatgata atcaggaaga tattgccggc cttagccttg acggtactcc
    13621 tcagctactc cggtcaggca cagcttatca ggaacagcgc tacgctgaag gatatgcaac
    13681 agatgtttga aaagcagaaa cagttggctt caggccgcag caatgagctt ttcggaatat
    13741 tcaaaacgcc cctgacgggt gatgagaagc aggcgcttca atacttgtat gcgtacatgc
    13801 cattgagcga cctcgccgat tacaatggca aattcttcct gaaggcggta agaatttcac
    13861 tgaaagcccg caacgagatg ccctggggaa agaaggcgcc cgaagatgtt ttcctgcact
    13921 tcgttttgcc catgcgtatc aacaacgaaa acctcgacac tttcagggtg gccatgtatc
    13981 cggagttgaa agcacgcatc aggggaatga acatgtatga cgccgctctt gaggtgaacc
    14041 attggtgcca tgaaaaagtg acctaccgcg gaagcgatga gcgcaccagt tccccactgg
    14101 cttccatccg gacttctttt ggccgttgcg gtgaggaatc gacttttacg gtaactgcgc
    14161 tccgcaccgt cggaattccg gcgaggcagg tgtacacccc ccgctgggct cattgcgacg
    14221 acaaccacgc ctgggttgaa gtatgggttg acggcaaatg gtatttcctg ggcgcctgcg
    14281 agcccgaacc agccctgaac atgggctggt ttgccgagcc tgcccgaagg gccatgctgg
    14341 tacacacaag agcctacggg cattataacg gcaatgaacc ggtcatcgaa aaccgggagc
    14401 gttttgccga gctcaacctg atagccaact atgccgaaac aaaacccatt tttgtcaggg
    14461 tagtggacgc atccggtaag ccggtgaaag atgcgactgt agaatttcag ctatacaact
    14521 atgccgagtt ttacccgatc gcacggcaaa ccaccgacag caaaggagta tgcggcatca
    14581 caacgggcct gggcgacctt ttgctatggg ccaccctgga cggaaaattc gattaccgta
    14641 agattacagt tgaatcgacc gacactgtag tccttcagcc aggccggccc catgctgccg
    14701 gagaaatctt tgacctggtg cctcctgttg agaaaacccc gctcgatagc aaggtaaccg
    14761 accttcagcg cagccgtaac acacaacggt taaatcatga agattccatc cgttcggcct
    14821 atatggccac tttcaccgac tcggtgcagg cggtcgcctt tgccggcaag ctgggtatca
    14881 gccgcgaaaa agccgttaaa tacctcaccc tgagttatgg caacagcaag gaaataaaat
    14941 cgttcctcca gaacacgcct gcaggcaaga tgcaatgggc aatgtacatg ctgggagtga
    15001 taaccgaaaa ggatatccgg gatacccggg aatccatatt gagggatcat ctggacaatg
    15061 cctttaccta cgcaggcagc aacgaacccg ccgacaggga atttttcgcc cggtacgtga
    15121 tgtcgggacg gattgccaac gagatgatgc ttccctggca tgccttcctg cagaagaaat
    15181 ttaacgccga atttgcggca aaggcgcaaa aagacatcaa cataattatt gattggatca
    15241 aaaccaacat ccggatcgac aacaccgcca acctgcattc acgttctccg ctttctcccc
    15301 gtggcgttta tgaactccgc gttgccgaca aacgttcacg cgacatattc tttgttgcat
    15361 tatgccggag catggccatc ccggcccgca tcaacccggc aacaggcatt ccgcagtacc
    15421 ggcaaaacga tgattggcag aatgtggctt ttgaacccgt cgcagaaaat attgcccaaa
    15481 aaggtttcat ccgttttgtg aatggtaatc cggcaacaga cccaaagtat tacatcaatt
    15541 ttacggtcgc acgtctgtcg gatggcgtat accgcaccct ggaactggca gagggaaaat
    15601 cagtcagcga cttcaccgaa cccctttcgg ttgaagccgg cgcttattac cttgtgacgg
    15661 gtaaccggca ggccgacggg agcgttttgt catcaattac tttcttcgat gttccagcag
    15721 gcaagaccat tgatctggaa gttaccatgc gcgaatcgtt cgaagagcaa aagccctggg
    15781 gcgcgattgt tcctgacaac ttcagcctgg aaaattatgt tgataaaaag acgctgaaaa
    15841 tgagcgatgt catgaaccat aaaggcgtca tcctggtgtg gattgatccc gacaaggaac
    15901 cgtcgaaaca cgtaatggct gatattcctg cggttcgttc cgtcatcgaa aaatggaacg
    15961 gaggtctcgt ttttctgctt acagaaggca ggataacaca ggccttcaac cccgaaacat
    16021 acaaggacct gcccgcccac agcatttttg cttatgataa acagggaaag cttcttgatg
    16081 ccgttgggaa aataaaaggc agaaaacttg atgccagtat gcctgttatc atgatttcgg
    16141 ataccggcgg aaacctgttt tatttctccg aagggtataa aatcggaatt ggcgaacaac
    16201 tggccaagga aatcaaccgc atgaaataga aaacggttcc tggtccgttc cggggaatcc
    16261 tttacgaata aaggaaacgt ttgatacttc ttttgggagt tttggcaaac tctttatcct
    16321 ggatttcgaa gccggcgatc gagataaaat gcggcacctg tttgttgaca gccttacggt
    16381 gatgttcaaa cagtttttca agatcctcac gggccaattt ctcatgggcc accacatcct
    16441 gatccggata cagtaatgct atcagtttat tgttgcggct tacaatcagc gattcgccga
    16501 taccatgccg gttattcagg cggttctcaa tttcgtcggg atagatattt ttgccgttcg
    16561 ggccaaggat catactttta ctgcgtcctt tcagatatat ccgtccttcc gcatcaacca
    16621 gccccaggtc gccggtatgc agccagccgt tggcatcaat tatttcggcc gttaattcgg
    16681 ggtttttata gtaccctagc atcacattat cgcctttcac cgaaatttcg ccgggaatat
    16741 tccggggatc gggtgaatcg atcttaagtt ctagggtgtc gacaacctta ccggaagccc
    16801 cgatccgtgt ttcgcgccag ttttgataac agatcagggg gccgcattcc gtcatgccgt
    16861 aacccacggt aaacgggaat ttgatcttgt gaaaaaatct ttctacctgc ttgttgaaag
    16921 gcgcgcctcc gatcacaatt tcatgaaact gcccgccgaa cgccgatacc agtttttccc
    16981 tgatttttgt gtggatcttg cccctcacac ccggtatcag cagcagcact ttcatgtacc
    17041 atttactgat cacaggctga atctgcttct tgaaaatttt ctcgatcacc agcggaaccg
    17101 acaataccag ggccggtctt acctcctgaa aagcctgcaa ggctatctga ggggaaggcg
    17161 ttttggtgag gatggtaatg ctgcatccca gcgtaaaagg aaataaaaat tcgaaagctg
    17221 caccgaaagc atgcgccagc ggcaggaagg aaacaatttt atctcccggc tgcaaaggca
    17281 tgttatgttg cgcatactta atgtttgcag caaggctgtt gtgctggagc atcacgcctt
    17341 ttacaaatcc ggcagttccc gaagtatagc tgatggcggc aagcctgtcg tttggaaccg
    17401 gatcaaatgc aagcttatcg gctgtaagcg aactttcgga caccggtttt aaaaccgacg
    17461 actgttcata agcagctttg atctgatcat tgccggaatg gatcatagcg taatcgaaaa
    17521 ccgaaaaaat gcctttcacc gaagggagtt gctcaggccg aagatctttt gcaaatattt
    17581 cgtcggcaaa aagcatcatt gcatcagaat gattgagaat tccgtgcaaa tcttcaggct
    17641 tgaaatcggg aagtatgggt acaattacag ccccataggt tacaatagcc agataggtaa
    17701 tgcaccatcg ggctgagttt tttcccacaa gggccacctt atcctgctgg ctcacgccgg
    17761 ccagccgtaa aaattcatgc aggcgcgtta tttgtacggc tatatccgag tatttgtacg
    17821 attcaccatt gtagtcggcc aaggcaggga gttcccaatt cttcttgagg ctgtcttcga
    17881 tatatttaac gaaattttcc tgaagcatcg tcttatttta aatgagccgc caaagatagg
    17941 tattgcgatg tgggattgtt ctctttaatt gtgatatttt tctgaattat ttattatatt
    18001 actgatctgc tgatcgttat acatacataa tcctttatca tttccactta agtcatgacc
    18061 tgactgaaca acagcccggc cggagattga attgagacag atagtaaaat ttttgagatg
    18121 gtgagaagaa gggatatgat ggttgaatca taattttaaa tgttaattca taatggacca
    18181 ctaaacccgg aaaccatgaa acctttcagt tatctgcttc cagcaatcct ggccgccggc
    18241 atgatattgc tgataacatc ctgcgacgac accggcaaca aagctccgtc catctcttcc
    18301 gtcaggataa atcccgacag tgtgaatgca ggaggtacgg tcaaggtcac agtaaatgcc
    18361 gttgatccgg aacaggaccc catcacctac ttatatgaag tttcgggggg aagcattacc
    18421 cccgacggtt cctatggtta ctggagtctt ccttccgctc cggggacagc aacagtagag
    18481 gttacggcta ccgattcgga gggaaatagc tcctcaccaa ccacaatttc agtcacggta
    18541 ctagcccctg tcacccagat taccggttac gccaaacttg atgcagggta caccggcaat
    18601 ttagaaggat ccgttgttca tttggaaaca tacggagccg gattgatcat aaaaaccata
    18661 acagcaaccg gcagcggctc cggaattgtg ttcaatctgg ccggcatcga cccgggaacg
    18721 taccagttga tcatctggaa agacaccgat cattctggga acccgaattc aggagactat
    18781 gttggatggt acggttccgg aggccccctc agccccgatt tcgaccctat tgagattacc
    18841 ggaggacaaa actttatttg caacatcagt atgacacatc ctgccaaata gagccaggtc
    18901 aggcatcttc acctttagcc cctgatggcg gttatattgc ctgtcacaaa catattcatg
    18961 gttggtgcaa tttcccatgg cggggaagta cggggcagaa gaagggctgg ttcaactgct
    19021 gcaaatcaat tttatatccg gttccgacgg gaatggcagt cacttcctgt cgccactttt
    19081 gcattataag ccgcttttta tggcaaccgg cggaattctt cactaaattc gtcttgttgt
    19141 tacctgtacc ggattccgca atgatcctcg aaatagctgt atactccctc gaagctgcca
    19201 ttactgccca aaaagcagga gccggccgta ttgaattatg cgccgccccg gccgaaggcg
    19261 gtcttactcc cagcgccgca accctgaggc tggcccgtaa ataccttaaa atccccatcc
    19321 atgtgatgat aaggccccgc gaaggcgatt tctgctattc cgatcttgaa ttcaacgcca
    19381 tgttgctgga tatacaggct gcaaaaatcg caggaatgga tggcatcgtt gccggaatat
    19441 tatcgaagga tggtaccgtt gatacgggcc gcatgcgggt cctggcagaa gcagcagctc
    19501 ccatgccggt aacttttcac cgcgcctttg acatgacatg cgatctcttt gattccctgg
    19561 aaaaaattgt cggatgcggg atcaaacgca tcctcacatc cggaggccgg caaacggcct
    19621 gcgaagcatt gccggtaatc aggcggctgg cggaagctgc caacggaaga attagtatca
    19681 tgcccggcag cggcatcaac gagcacaaca tcagggaggt cattgaccgg acaggcgttt
    19741 cggaaatcca cctttcagca aagatgcata tccccggcaa catggaatac aaaaacactg
    19801 ccgttgccat gggaggaaat gtcatcattc ccgaatacga tctgctcctg cctgatgccg
    19861 atgcaatcag ccgcatccgg aaattaattg actgaaaata atccgtttta atcaaccact
    19921 cactgatatg gaggaaacat caatccctgt cctgtacgat acaagacttt ttaccaggat
    19981 atggtctccg gcaaggtttc cggcaggcat tgtatgtttg gtccacgggc tctccgatca
    20041 cggcggaaga tttgtccatg ttgccgaagc gctggccgca aaaggctatg cttttattgc
    20101 tcccgacctg cgcggcaacg gccgttcccc gggcagacgc ggccatttca attcgcttca
    20161 ggaggtgatg accgacctaa aactgattct gaaagatgcc agggacaggt tccccgggtt
    20221 gcccgtattt ctttatggcc aaagcatggg cggaagcctg gtgatcaatt ttgtgttacg
    20281 ctttcccgaa gaggttgacg gtataatttc atcaagcccc tggctccggt tagcaaaacc
    20341 tccttccgga ttcaccgttg ccgcggcttc actgctcaaa ccgttttttc cttccgcact
    20401 ggttcccaac ggcataaaat cagccgatct atgtcatgat gagtccgtac aacatgctta
    20461 cgaaattgat ccgcttatac actggaaaat ttcggtgcga acctttttta tcattcagaa
    20521 atacggcgaa tgggcaatac aacatgccgg tatgcttaaa aagccggtcc tccttatgca
    20581 cggtgacgcg gatagaatta catcctataa agcaagccgt gattttgcgt ctgcctcaaa
    20641 caaccatctg aattttaaag gctggcccgg gctttttcac gagttacata acgaacccgc
    20701 ccgggacgaa gtactggctt acgttagcga ctggttggat cgctgcaggt tccgccgcct
    20761 gtaatgcaca aaagcctcct gtgaggcttt tatgcaggaa aactaaaatc ataaaaggct
    20821 atttatcagc atcaaaccgg tacaggctgc cttcgtacgg gaacctgtcc ggggtgtcgg
    20881 tcaacccgga agcggtgatg ccccggttaa cagtaatctt ttccctttga aggctttgtt
    20941 ttgaaatctc ttccgtatca aaagggatat catcagcata ttgttcaggc tgcagcacca
    21001 gaggaagctt ccctgacgcc gcgcttccgg caattattgc cgtattgaat ggaaaatccg
    21061 gggccgggct ttcatctttc aggttcagga taccagatac atccgaagcc tttaggcgat
    21121 agagtgttgc gatattttca gtattaaacg gaatatcgtc gatgtagaat tccggactca
    21181 tttccggaat ttcgatactc ttcagcttca gatttcttaa atagtcggcc gttatgcccg
    21241 aggtgctgaa aggaatatcg ttaacaaagg gctcttcatc cggtttcaac atcccggaaa
    21301 aggaattctg cagatcatcg gtaaaagcat taaagaagaa aggggaagta tagctggttt
    21361 tattgtgagg gtcgccgtcg gctgtagcat tgaagggatg attgattgac agaattcctg
    21421 ataaaagaat aagtgtcagc gttttcataa ttctttttgt ttaaaagtta ctgttttatc
    21481 ggttctgtaa tatgacggct tgtctattga attgttacag aaaaaattgt taacgaataa
    21541 ttaagacctg cctcgtaaaa ccggaacttt tcatcctggt cgggataatt cgtgttcgcc
    21601 gggcaatacc agcaatgcgg tgcctaaacc tcattcactg aatcgcaaac ctgcctctaa
    21661 acacgttaaa gcctgacgac taaaaacata cgagggtttt tgaggcttaa aatctgcgtg
    21721 aaagcgatca ggcgatttac catctggcgg gctgctgagt tctcccgata aatacaggca
    21781 ggcatgctgc catccaaccg gttgaatgac atatcattag aaaccatttg acaatcaggc
    21841 cggacagaag cccgttagca acagccaaaa tgcagtgata ccaaggcatc tgtgagttta
    21901 acatttatta gaattccatt taacatcctt taacacagaa attataatac atctccccaa
    21961 gcggcagtta ctttgcatgt catttcaaaa gaaccaataa taacaaattt gaaaatgaaa
    22021 aaaatcgtag taacattagc tgtattgttc ctgtcgggaa cgatcgctat ggctcaaact
    22081 caaaaacctg ttgagaagaa agagcctgtt aaaactacaa ccatgaaaca ggaagcagca
    22141 aaaccagtag ccggacaaaa aacggcgaac gtgcaaacaa agtcacaatc tgtggctact
    22201 cagggcgtga ttaaggcgga acccgcaaaa gccgcccagg catcaactgc gacaaaagcc
    22261 gtagtgcaca aacaccataa gaaagctcac caggcatccg gcgtttccac tgcaaaatcg
    22321 actcctatag ctgctcctgc caccaaaaca gcaccggcaa aggatgttaa aaatacatcc
    22381 aacgtccagc ccattaaaaa gtaaaagtta aaccactgaa ttgacaacac gaggctgact
    22441 cttacgggtc agccttttgt tttttaagta gcagaatgat ccgtcaattc ggaacatcct
    22501 tgaagtgatg gcccattcag cggcaggaca tatgagtggc gatgcgccgg aacaacagat
    22561 cagtcaggaa accctgatta cgctacaatc ctgccgttca ggatgacagt atccaccttg
    22621 ttgcttccga atgcataggg cataaactct acggatggaa tggcattcgt aatgaaaaca
    22681 ttggcttttt tcccgacagc gatgcttccc aactcttcgc tcacccccat ggcataggcg
    22741 gtgtttaagg tagttgcatt gacagcttct tcgggcgtca ggcgatataa gatgcatccc
    22801 atggacagaa tgagctgcat attgcccgaa ggggatgaac cggggttata atcgctggca
    22861 agggcaacgg gaagacctgc atcaatcatg cggcgcgccg gcgccagcgg catattcagg
    22921 aaaaatgcag ctccgggaag gattgttggc atagtttcgg tgtcgagcaa cgccttaatt
    22981 tcatcctcgc ccgtaaattc cagatggtcg accgacaggg cattgtattt tactccaacc
    23041 tggatgccgc ctgaacggtc catttcgttg gcatgtattt tcggcctgag tccgtatttc
    23101 atccctgcca tcagcatgcg ttcggtatct tcgatcgtga aaaagcccct gtcgcagaaa
    23161 acatcaatat agtcggccaa atcttcggca gccacctggg gaatcatctc gttgataata
    23221 aggtcaacat attcactctg atttccttta tattctgccg gcactgcatg ggccccaagg
    23281 aaagtagctt ttatggtcac cggagaaaac tccttcaggc tgcggattac cctcaacatt
    23341 ttaagttcat cctgtgtatt gaggccgtat ccgcttttaa tttcaacggc cccggtgccg
    23401 taacgcatca tttcctcaag ccgttcaagg gattgggcaa ccagttcggt ttcggcagcg
    23461 tcgtggagtt tcgcggcaga attcagaatt ccccctcccc tcctggcaat ctcttcatag
    23521 gataagcccc tgattttatc cacatattca atttcccggc tgcctgcata caccaggtgc
    23581 gtgtgcgaat cgcaaaaaca gggaaatacc attttcccgg ctgcatctat aattttcaac
    23641 tgcctgcctt ctctcgtttc gggcaaaaac aggtcttcca tcctcccgaa atccgatatt
    23701 tttcctttct caatcagaag aaaggcattg cgaatggttc ccaaatgccc catctcgggc
    23761 ccgcatacca tcatcctggg tttattttca acctggacaa gttccttaat attcgttatc
    23821 agaattttca tgcatgatcg atttaaacgt caaaggtaga aaaagtgccg gatgcggatt
    23881 atcccggata ccttattaaa taatgaatgt cggtgtaccg attaaaacca aaagatattt
    23941 tggattttca tatcatagaa ccacaatttg atatcgttac atttattact tttgtcaatt
    24001 aatcaaaaac acctcatact atgaatttac gaaaatttac tcttctcata acgttgataa
    24061 tggctttagc ttctgttgaa gcggcaattg ttgataaaga ggcagcccgt accgtagcgg
    24121 ggaattttat taaagaacgt atataccatc atcagatcaa ttggcaagcc tccggccttc
    24181 agatgacgga tgtactaacc tataccattg acggagttcc tgcatattat gttttttcca
    24241 acaacggaac aggcttcatc attatttcgg ccgatgaccg gatcacaccg gtactcggat
    24301 attccgaaaa aggcgtttat actgaaatag ggaaagccac caatttcgac tatttcgtac
    24361 attgttatgc cgaccaggta aaatgggtgc gtgaaagcgt ttatgatacc ccggccgatg
    24421 tcagcgcctc atggaataat tatctgaacg gaaaccttga ctatactccg aatagcacta
    24481 ccgacgcagg gcccctgctt cagtctatct ggaaccaatc tcctctttac aatgaatttt
    24541 gccccgaaga tgaagacggg cccggaggac acgtggttac cggttgcgtt gcaacggcca
    24601 tgagccagat catgtactat taccgtttcc cgctgcaggg aagcggctcc agaagctatt
    24661 acatgcccga ttacggaaca atttcggcca attttggtga aacatactat gattgggacc
    24721 agatgcttaa tgaacttact aatggaagcg gacaatgcat ccctgcagtc gctctcctga
    24781 atttccatgc cggtgtttcg gtcagcatga attatggccc cgacgcatca ggcgcccaga
    24841 gcgacgatgt tccttatgcc ctgaaaacct acttcagata ttcctcatcg gtcacctatc
    24901 aggaaaaagg aagcatgacc cagacgcaat gggaaaacca gtttactcct aaccttgacg
    24961 aaaagaagcc ggtatatatg tcgggacatg atcccgcaca gggcggccat gcctgggttt
    25021 gcgacggata ccaggttaac ggaacttcca agttattcca tatgaacttc ggatggggcg
    25081 gttataacga cggttactac tcgttgtcaa atccgcaggg attcaccaat tcgcaggcct
    25141 gcgtcaaaaa catctatccc ggcgcagcct attatcctta tagctgttca caggataccc
    25201 tcaccggagc catcggatca tttgaagacg gcagcagcgg cacagccaac tacggatcaa
    25261 atctgggatg cacatggctg attgcacccg ccgattctgt taaaaccatc acgctgagtt
    25321 tctccgcctt cgatgttgac gccagcgata tgctttacat ttatgacgga tcggacaaca
    25381 cggccccgct gcttgcatca tacaccggat caaccctacc cgccgaagta gtttcgaccg
    25441 gcaaccgcat gttcgtggta ttcacgaccg atggcgcgaa tgaatcaacc ggatggcatg
    25501 ccgaatatta ttcgcaattc ccctcctatt gcggcggtac tatcacgctt accgatcctt
    25561 ccggaacatt ttcggacgga agcggcaatt caagctacaa caataactca ctatgtaaat
    25621 ggaagatcgt tcctccgtat gccacaggcc tcaccctttc cttcaattct ttcaacctca
    25681 ctgaagggga tgaattgatg gtctactcaa ccggcggcac taccggaagc acacttatgg
    25741 gcaccttcac cggaaaccag attcccgatc ctgttgtttc tacaggaaca ggctttctgg
    25801 tcatgttcaa gagtgatacc tataatcccg gcgccggctt tgaagcccag tataccgtcg
    25861 gcaatgtcgg ccttgatgaa caagccggta tcaatggtat tgtgattgcc cccaaccctg
    25921 caaccaaata tacgtctgtc cgtttttaca acaagagcga cgaacctttc agaatgaata
    25981 ttgtcgacat ggctggcaaa gaactgtatc acgaatcttt tgcaggcacc tcgggcaact
    26041 tcgttaaaaa tatcgacctt acgggattca ataaaggaat gtatttcctg attatccgca
    26101 cttcagaagg agtgaccacc cgcaagttta tcgtgaaata agcataagtg tctgaatacc
    26161 ggaaagaggc tgtctcaaaa gggctgcctc ttttttttta atcggtcctg ttgtaacttc
    26221 gttgtcattc ctacgtcaaa gaattaaatt tggccggtaa taagcaatga aaccaggttt
    26281 aagacgatat ctgctttttt ctttcaacag ccttgaagcc ataatatggc tcacagcttt
    26341 gatcttgctg gcggcaacag ccaataccgg aacccattat acgctttgtc ccttgaaata
    26401 cttcggcttc gaattctgtc cgggttgcgg actgggacgt tccatcgccc tgatccttca
    26461 tggtcagttt attgggtcgc tccgtatgca tccactggga tttcctgcgc tggctctttt
    26521 attatggcgg atcctttccg tttttaccaa tgcattcagg ttatcccggg tacagcccat
    26581 acagaattct ggtcaacagg caaatccgtc aaccatataa ctacaaatca aacaattaaa
    26641 cctttccacc atgagcacac ccctttacca attgctgccc gaactcgata tgcaagagat
    26701 gggcttcatt caaggcataa caaaaaatta caccgaacaa caactgctga attttgccaa
    26761 catgtaccgg atcaggcgcc gtgatcctca gatcatactc cttaccgcat tagccggttt
    26821 cattggcatt gccggcattt agcggttcct ggtcaaccag attgggatgg gaattctata
    26881 cctgcttacc ggagggttat gcctcatcgg taccattgta gacctggtaa accatcgcca
    26941 gcttgccctt gagtacaacg ttcaggttgc ccacgaagta gcatccatgg ttggacaaat
    27001 gccagcctga taaaccattc caatccggtc cgcagtttaa caacttactt cggctcttaa
    27061 gttttttcaa tcaccccgtt acatttttct gcggggtgat tgtttttatt tatttttgga
    27121 aaaattttgc ccatgagata tatcttaccc attttcgtaa tttcattttt ctttgccgcc
    27181 caactttttg ccaaagaagt gactgtgtcg gacgcacgaa aagtcgccgg aacaattatc
    27241 cgggaaaaag ccccgtcatc aaatcaggat ttcacgctca gcgaaaatat gctgaccgtt
    27301 tcgatgaacg gactgccggt ttattatgtt gccgaccttt ccggaaacgg ttttatcata
    27361 gtatcggccg atgatgttgt tatgccggta ttaggatatt cgtttaccgg aggctacgat
    27421 gaaaacaacc tgcctgaaaa ttttaccgca tggatgcagg ggtatgccga acagattaat
    27481 tatgccaggc aacacaacct tcaggctgac gataagataa ccgggctatg gaaaagctat
    27541 ctcagcggac agcaaccgga aggtcagcgg gctaccaccg gtgtcgcacc catgcttacc
    27601 tgcacctgga accagggcgc gccctataac ctgctttgcc ctgccgattc taaaggtccc
    27661 ggggggcacg tttatgcagg gtgtgtggct acggcaatgg cccagattac gtattactgg
    27721 cgttggccat tacagggaac cggatcccac gggtacacct ggggtaatta cggatacctt
    27781 tttgctgatt tcggaaatac cgagtatcat tgggaagaaa tggctaacag ctccattggc
    27841 tttaactacg aaatggctca gttacagtcg catctcggca tctcagtcaa catgatgtat
    27901 agcccggatg gttcaggcgc ctacagtgaa gatgccgcca ctgccctgaa aacatatttc
    27961 gggtattcgg atgacctcac cctggtttat aaagatgatt acaccgatga acaatggggc
    28021 aatctgatgc gccagcaact ggatgccggg caaccgatgt attacgacgg ctatggcaca
    28081 ggaggacatg cctttaacct tgacggctat tccgatgacc tttattttca tttcaactgg
    28141 ggatggggcg gaactgcaaa tggctatttc ctgctttcga acctgaatcc ggcaggcaac
    28201 aacttcagcc agggacaagg cgccattatt aattttgtac ctaacagcac ttacccctat
    28261 tactgcacgg aagcaaacac attaacggcc aactatggct ccctcgaaga cggcagcggc
    28321 cctttgcagc cctatctcga taataccggt tgcggttggc ttattgctcc ccaagactca
    28381 atcagggacc tccagcttac cttccatcgt tttaatctcg aagaaaatca cgatttcctg
    28441 accatctatg acggatctga tgatacagcc ccggtgctgg ccaccctgac cggtcaatcc
    28501 ctgccgccgg tcattacagc taccgacaat aagatgtttg tcagttttac aagcgacaac
    28561 gggggaaccg ataacggttg gttcgcttcc tattcagccc aaacggtaag tttctgtccg
    28621 ggtgtcacta cccttaccga gccgatggga acgtttaccg atggcagcgg aacttatgat
    28681 tatcacaaca acagtgtatg caaataccgg attatgcctc ccgacgcccg gaacatcact
    28741 ctttacttca acgaattttc cactttcgac gaaaatgatt tcctcctcat tatcgacatc
    28801 aataccggtt cgacattgta ccagctctcg ggaccggaaa ttcccggtcc cctatatctc
    28861 aacacgggta aggtattgat actgttcaaa acagatccat ctaatactgc gtcgggatgg
    28921 aacataacct accaggctga tacatacaac ggttccgaaa atccccgtga aaacagcctg
    28981 ttcgtttacc cgaacccggc ttccggcttg ctgaatatct ctttcccatg tttcccgggt
    29041 gaaaagcagg ttcttactct taccaatgct gcaggtcatg aagtattggc cagatcggtt
    29101 tcctccggga atacactggc cgatgaacgc attgacgttt ctggttttgc ctccggtatt
    29161 tatgttctgc gcctgataaa cggtaaaagc gtgattaccc ggaaagttgt gatcgaataa
    29221 ccattttccg gcacataaaa atgcagcaat tgtaatccgg aaagaaatcc cggagacagt
    29281 tgaacacagg atagatcagg aacacgctcc tcttgcaggg aacttgccta tcaaccgctc
    29341 atccggaaca ggttgaaata ttttccatca ttgaaattca acctgtttcg gattatttta
    29401 aacaaaatca accttgcttt atatcattaa tttttatatt taactattac ttttgtcgac
    29461 atttaaaata aataatagat atatgaaaaa ctttctgttt acggtcatgc ttctcagcat
    29521 cgttgctgct gcaaactgcg ggattgtcac acgtgagact gccgggcgtg tggcagctaa
    29581 tttctgttat gaaagttgcc ctgccgccag acagtcagga tactgtagta taaaaaccgt
    29641 gcagattttt gaaaaagatt ccggtgcccc gttgtattat atctgcacgc tgaataccgg
    29701 aggatttgtc attgtttcgg caagcgacaa tacagttcct gtccttgctt actctttcga
    29761 caactcttat gatcctgaaa atatttcctg ctgtttttca tcgtggatga aacactatga
    29821 aaatcagata catcaactga taatttccgg taatgaagct gttcccgcca tttcagaaga
    29881 atggaaaagg ctggcatcgg ataatccgga gaatttaagg gataataccg gcctgttgtc
    29941 ggtagcgccg ctccttgtga gcaaatggaa ccagggaagc ccttataatg ctatgtgccc
    30001 gcctgatcca gccggtcccg gaggccactg ttatgcaggc tgcgttgcta cggcaatggg
    30061 ccagctcctt tattattaca ggtatcccca acagggcgca ggatcatacg cttattacct
    30121 tcccgattac gggacaattt ccgctgattt cggctcagca acataccagt gggacgctat
    30181 gccatcgtac ctttcacgtg aaaacgacgc tgttgccacc ctcctgtttc accttggagt
    30241 ttcggtagat atggattacg gccctgacgg aagcggaatg tggaaccata aagcagccta
    30301 ttcgctcaga acttattttg gatacggccc cgaaactaag tactatttcc gcgacagtac
    30361 caatctcgac tgggacagca ttctggttgc cagtcttgat cagcgcaagc cgttgtacta
    30421 tgcaggctgg gaaggggtac aatccgataa cgggcatgct ttcgtatgcg acggttacca
    30481 gggaaccggc tatttccatt tcaactgggg atggggcgga tcaaacgacg ggtattttta
    30541 tctcgataac ctgacgcccg gcggcaacaa cttcaattat gcccaggaag tgatcccttt
    30601 gtttcctgat accctttcat cagactatcc gccttattgc tcaggtaaca aaacgctgtc
    30661 atccatcgac ggttcggttg aagacggaag cggatggttc cggtaccagc ccggatcgga
    30721 ttgctcatgg ctgattcagc ctgatgacgc agaacatgat tcggtttcaa atatcaagct
    30781 gatattcaat gccattgata cggatacatc cgacagcatc gtggtttatg acggcgatac
    30841 cacaacagct actgtcctgg gttcattttc gggtaatact cttccggcgg agataacttc
    30901 aggcagcgac cggttactgg taacattcag cagcaatgcc agcaacaacc tgggcggatg
    30961 gatagccgat tacagaagca cattgccggt atattgcagc gggcaaacca ccctcaccgc
    31021 cacttccgga tcgttcagcg acggcagcgg cgataaacgg tacaacaaca attcggtatg
    31081 caaatggcgc attgaacctc ccggcgctga ttttgtcacc ctctggttta cgtccttctc
    31141 aactgccgac actcttgaca aggtaattat tacggacctg gtaacccatg aacaattagg
    31201 ggaattctcc ggttctgaaa tgcctcctcc cgtgactgcc accagcggaa aaatgttgct
    31261 catattcttc accagcaata caggaacagg cgaaggatgg gatgcttcct atgtgagttc
    31321 aggcgcaggc gaaggcgaac cttttgcaaa tagcgaagac atttttcttg ctcccaatcc
    31381 ggcaaatgac aatactttcc tttattacca atgcaagcaa ccaggattgg tgagccttga
    31441 aataaaatcg ttaaccggaa aaaccatcag aagctgggat tatcaatcgg tcaccggtat
    31501 gaataaaata ccggtcggca ttgaggacct ttcttcttcc gcttacgtat tatttctgac
    31561 atcgaaaaac atgaaaaaag ctgtccggtt gatagtaaat taggggctaa aaaattttta
    31621 tatgcctgat ttactgtctt ttagtttatt tagaccaaag aaaataaaaa aatgattgta
    31681 tcggctttgt cttttcgtat atttagtacg aaatttgcag gttccacaaa caggttaacg
    31741 aacttattga acctgttata ccggaaaaaa cactaacaca aaaatcatat aaacctcaaa
    31801 cttgaaataa tcattaatta cacctaaaac aaatagttat gagaaagtac gctctattgg
    31861 cagtaatcat ttttctgaca gccgctgcaa attttacgac tgcacagaat tcggatcaca
    31921 aatgggcctt gggtattaat tggaactggg aagattttca cacagtaaag cgtgatttcc
    31981 ccgatcaatt caaatataca aggtggcagg gatataaatt tccctcggca atttcactcg
    32041 gccgttacat caacccgtca ttcaatgttt tcggcgcatt cgggctcagc aagctggaaa
    32101 aagaatccat ggcagaaaca atggtaaatc agcccctgtc ggatgataaa ttctggaccg
    32161 gagaccttaa cctggcctat aaatttgcaa acggctacat cctgaaagag acttcatggt
    32221 tcgatccgta cctatacctg gggctcggag cttccagcat caaagataaa gatcaggaag
    32281 atggcaagac tacctatttc aaaggtgatc tcggcctcgg catgacattc tgggcaactg
    32341 aatggttagg attaaacgtt cagggcgctt atgattatat gatcacccca aaactcaacg
    32401 acgaaaggcg ttctgattac atgcattaca ctgccggact gaaattccgt tttggcgctc
    32461 ccaaggatac cgatggcgat ggcgttcctg ataaaacaga ccgctgcccc gaaacccccg
    32521 gtaaagttga gttaatgggt tgccccgata aggataatga cggtattgcc gatctcgacg
    32581 acgcatgtcc cgatgtagcc ggcaaacccg aattcaaagg ctgccccgac accgatggcg
    32641 acggcattat cgataaagac gacgcctgcc cgacagaagc cggcctgaag gaattcaacg
    32701 gatgccccga taaagacggc gacggaatca tcgacaaaga cgaccgctgc cccgacgtta
    32761 aaggactgaa agaattaaaa ggttgccccg ataaagacgg tgacggaatt gccgacatcg
    32821 acgatcgctg ccccgatgta aaaggcctga agaaccttaa aggttgcccc gatactgatg
    32881 gcgacggaat tgccgatatc gatgataaat gcccggatgt tgccggtacg ttagctaata
    32941 acggttgccc cgaagtaaag aaatttgaac tttacaaagt cgtttatttc ggctgcgaca
    33001 agagtgtggt ccttaccaag tataccaaag acctcaacga agttgccgat atcctgaaag
    33061 caaatgccga tgttgccgtt tctgttgaag gctatgccga ttcacagggc cccgatgcat
    33121 acaacatgaa actttcggaa aaacgtgccg attatgtgat caactacctc gtgaagaaag
    33181 ggatcagcaa agcacgcctc aacaaagcat tctttggcga aagcaatccc gccggcgata
    33241 acaaaactgc tgccggtcgc gccttgaacc gcagggttga aattaaaacc aagtaatccg
    33301 gttttatcaa ttataaagaa ggccgatcta accgatcggc cttctttttt attttaatag
    33361 ccgcctgggt caggctcccc gaatccccgg cgttgattac aactcctgat tctatatcat
    33421 tgcctgccgt cataagccgg ccggttcaac ggccaatatg ttctgcatat tccggcttct
    33481 gtttttaatt gccggttcct ctgacttttc agccctttct gtaccagtta gtaacatcat
    33541 cttttttcaa cctttctgtc tcaggctcca cgctttcagc ggcaagcacg gtaaaggaag
    33601 aatgcaatta tttctggtta aagaatctgg tacggaatgc ggcaataaaa gcatcatggc
    33661 tgtaaaatgt acaatttacc tatatttgca aacgaaaaaa ccgcccggga cgttgggcgg
    33721 tcttcaaata agtggactca gatgtcagga tcagatgtca ggagaagtaa tcgcaaataa
    33781 aacctgccat ctcaacccgc caattatctt tattggcaaa ttttttaaaa aggccgcccg
    33841 ggacgttggg cggccttcgt tctaagtgaa ctcagataac agggaaagta atcgcaatac
    33901 aaacctgtta tcggaattaa ctattatcat catttctttt tccatgaagg agtattattt
    33961 attaggtcat tggttatttc aaagcagaaa tggcttttgt agctacatca tttttcggat
    34021 caagttccaa taccttcagc caatagctcc tggaattggt ttttgcagtc tctttcaggg
    34081 tatcatcctt ggctgtttta gcctcctcga actggagata ataatagtac cccatgtaac
    34141 ggtatgcctc aatgaggtct gtgctatatt tctgcctgtc atttttaagt tccaggatcg
    34201 caatggtttt ctcataatca ctttttgcca gcccctggat agtttccgga tccagccctg
    34261 acagtaccct ggcccgccat tggtatccaa gatatgagtt tggcgagatc acaacaaccc
    34321 tgctaaaggc cgaatcagct tttatcagat atggctttcg gctggctgaa tcagaggttc
    34381 cgctaaactg gtttgccagg agttgataat ccttacccag attgaagtaa actgatgatt
    34441 gaactttgcc cttctttgct tcaataaatt tttccagata attgatggat gcagggtagt
    34501 ttttctgttt atcgtacaat ttggcaacat cttcaagcaa gtcgtattgg gtggtatcca
    34561 tttcgaaagc tttggccagg tttgaaacag ccagcgtatc gttgccggtg cgcgcgagca
    34621 atctgccata attctcataa tccgtaaaaa tcaccttatt ggtatcgacg accgatcgta
    34681 cctcaaaaaa ctttttcatc gcttccaatc cttcagcatc gcggttgagt tcgtatgccg
    34741 tataaccttt cagccgaagt aaaaccgggt tctcaggttc gccgggcagc aatgtattga
    34801 ttaattcgta agcccgggaa tattctttgt tgaaatacag tatacttatg taacgggcca
    34861 tatccttccg gctgtattcg gccagtccga tatacttccc atacatttca ccggctttat
    34921 cgtatttccc cagcgtataa tacaattcgc ctgcgccctt taatgcaggg atatacaaag
    34981 ggtctttcgc gagcgccagg ctatactttt cttcagcggc ttcatagttt ctggtaccaa
    35041 aataaatatc tgccaggccg gttaatgcct caacattgtc aggctggata taaagcgctt
    35101 gctcgaaccg gccggaagct tttccgaaat atatcggata gttgtatttc ccggccagaa
    35161 tcaggtacaa ttttccggct acattgtacg attctactga ttttttatcg atggataatg
    35221 ctgctgtcaa tacatcggtc catacgcagg tatctcctgc catgaccatg ttctcggcca
    35281 cttcgataag tgtttttgtt tcctttgcca tggctgcttc tttcctggtc ttttgtaaca
    35341 gaagcatagc ttcctggttc ctgcctgcca ggttctcgct gatggcttgt cctaccatga
    35401 taagccggct tttcggatcg gcggctgcaa gtcttgaaaa gcaataattc gcagaatcgg
    35461 cattatactg ccgggcataa atttttccca gataatacca actttctgca gaattcatgg
    35521 ttttcagatc tgctttcagt acggatttag ctttctcgta ctgctccata tcaagcagtt
    35581 ttgcagcatt gccgttatcc tgcgcccgca acgaaagtcc tgctatcatc aggccggtaa
    35641 taagaaaagc gttcagtatt ttcatattgc ttctgatcta aatgagtatt tatttacttc
    35701 catctccgat tgccttccga tgaatttatt cttctactac atgtacgatt cttaccggct
    35761 gggtaatagg caatactccc gttttttgaa tgatacgctg tcctttgtcg catgaaacaa
    35821 aggaaacaaa ccctgaaccc agtccggcgc ggggttctgt gaggagggca taaacaaaac
    35881 gggtaaaagg atatttcccg gttgcaatgt atgcctgaaa cggctgataa gaagtttcag
    35941 gcaaagccgg gtcttcggac gaaatacccg ccactttcac tttttggaga aatgaaaggc
    36001 atttagggtc gcgactgttg ctcacccaac ttaccccgat aagcccgatc gcatccggat
    36061 tttttgaaac ataatccact acatccaggt tatactgcaa ggctgagagc tgatcgccaa
    36121 gttgtcctgt cctggtaact gaatcgacca tgaaacggac aatacttgaa ttcggattgt
    36181 cgaacaggac ttttattttc cccagcggcg atttcctgtt tagttgcttc cacgaagtaa
    36241 tttccgaaag caggattttt cggatagtat ttaccgagaa aagtgtgtcg ggatttgatg
    36301 ggttcaccag cacggcaata ccgtcgacag ccagttgcac ttcgtgcggg aagaatttct
    36361 tactatggaa gaaatttatt tccttagccg aaaggggccg cgatacaatg gccagtctta
    36421 cgctatccct caacaacatg ttaatcgcat ctacctccgg cacataggcg gcttttatac
    36481 tagccccctt gtacatggca tgaaaaacag ggagttcagg ctcgataacc ggttgaaatg
    36541 tttcatcaac agctatcgtg atctgtcctg tagtcggggt gtccgtatat ttatctttcg
    36601 cctttttcgg tttgcagcct gtcgcggtca gcaacagcag gatggttaat gcgatgaacg
    36661 aatatttcat tgctcagtta attcgttatc attgatgtta cccggattgt tattcacagt
    36721 atattcctta taaacccggt aagcccggaa aaccccatag accaagatga ggattcccag
    36781 tccgtggtta agacgcggtg aaagcataaa ccatcccctg aagagaagaa acaggcccat
    36841 aacgacgtag attatggata tactgtattt tattattttc atcctatacg ggttgttatc
    36901 attgggacgg taatataagg gccaacaccg ttttaacctg cgagcgtgaa cttgatagga
    36961 aggttaaact ggacaggaac agctctcccg ttttgtttgc ccgggatcca ggccggcatg
    37021 gctttgataa ccctgatggc ttcttcatca cacccaccgc caatgccacg caatattttt
    37081 acatccgaaa tttttccggt tttacttacg acaaaagttg tgaaaacggt tccggtaatg
    37141 ccgctttctc tggcgatttg aggatacctg atattatcct tgagaaattt attcatactc
    37201 gatacacctc ccggaaattc agggatttgt tcaacaacga taaacggctt ctcctcctct
    37261 tcctcttcaa caacctgagt ttgctgttgt tgtaaatcag caagatctac ggcatcggca
    37321 tcctggctgt ttcctttcac atccgcggtg gaaattgaca gtttcgattc ggagacttcc
    37381 tcctgcgtct tcatgacttc ttcatctttt acctcagcat cgtctttgat taccggcggg
    37441 gtaaatttaa tggtgctttt caccttttga ggaagttcct gctcaatttt cggctgttcg
    37501 atctcttttg ttttactttt ctccaacttg atgttcgaca aggtggttac ctcgacattg
    37561 gtttcctttt cagccggaat aatgcgtttt atgagcccgg ggagagtaaa tacaagcagg
    37621 aacagaatga tagtcgttat gatagcaata cggtggcgct ttgaagaaag cgaccgcata
    37681 agataagcgc catattcctt tggccttgat tcgaaaacca gattacacca atcgtcagaa
    37741 aatatattga tttttgccat gatggttctg tttaattctt tgacaattgg ttaccaggga
    37801 cctgagcctt taaaaggtcc tgatccgggg cttctatatt tacgactgca tacctgctga
    37861 tgctgcaaat attcatttca tcaagcgcat cgaccagatt cttgtagtta gcatcatcag
    37921 tagccataat ccggacaaca ggcgcatttt tgttggcctt gatttctgaa agccgcttct
    37981 gataatcatc atcattcata tgcgttttcg ttttctcact tttaaggtct tccacctgtt
    38041 tagcaacatc atagtttttc ccgaggagga ttttacggag gccattggcc gaataatcgg
    38101 ttttcaacaa tttggcccct tcggttttcc cgaaataata ataaacgaca ttatcttttg
    38161 ccagtatcaa agaaatagcc tgagaagctt tcgctactgt tttgtcttct tcgtctacct
    38221 tcttatccga cggcatggtg atttccatcg tttgtggttt tatgagggag gttgcaagca
    38281 taaaaaaagt aataaggagg aatccaaggt caaccatcgg ggtgaggtct acccggggat
    38341 tcattttctt ggatcttttc ttaccgcctt ttttatgtga accggcatct tgatttaatt
    38401 ctgccatgat attacctatt tacacgttaa atttattctt tcccgggagc cccttcaagc
    38461 cttgtgatca gattgtagcg gttctcatcc aactcctgga ggctggtcat aacctttctg
    38521 ataacaggat aaggggtggt ttcatcggct ttgattgcca accgtaaatc gggattgatt
    38581 tcgcgggctg ctttcaccca atccttgaac tcattattgg tggaatcagc cggtataccc
    38641 aggttattct ggggcaggtc cctcacttcg ggctttaatg ataagtaagt ctttaatgct
    38701 tgcatcgggt tacccacaag gtctaacacg gtatagcact taatttcttc aggagtaaga
    38761 ttcaccttgt aggtttctgc catcttctcg atgagaagtt gtctgttttc ctgcccgtca
    38821 attccaaagt acactttccc ggttttatcg atgagtattg tcatcacgtt tgtttccggg
    38881 atcttgatct ctgaaaccga cgaaggcgtt gataccagca ccggttcttt ctttacaaaa
    38941 tttgaagtaa gcataaagaa agtcagcaac agaaacgcaa catcacacat ggcggtcata
    39001 tcgaccaccg tgctttttcg ctttattttt gctttaggca tctagcttat ctttagttac
    39061 ttttaagact aagcatggtt tgcagcatag gtctgaacca ggctgaatcc ggcttcatca
    39121 atgctataag tcagtttgtc aatcttcgtt gtgaagaagt tatagaatat aagggcgaaa
    39181 gctgcagtac caataccaaa agcggtattc accagcgctt ccgagatacc ttgtgcgagc
    39241 gctaccgagt cgggagcccc ggcgttggca agagcggcaa aagccctaat cattccaagc
    39301 acggtaccca gcaatcccat gagggtcccg agagaagtga taacggccag aaatacaagg
    39361 ttttcttcaa gagcaggcaa ttccagcgac gttgattctt caacttcact ctgcaatgcc
    39421 gagagtttct ggtcctttgc catcgtcgtg tccttttcaa gtgtacgata tctcagcaat
    39481 actgttttga taacattggc taccgatcct ttctgaacat cacattcagt catggccccg
    39541 tcaatttcat ttttcttcag atcatccttt acgatctgta caaaatcggc aacgttgcca
    39601 gtccctgtcg ctttattgat ggcaatgaag cgttcaatta caaacgttat gactgtaagc
    39661 agaagggtca tcaggatagg aacaataaaa cctcccttat agatgattcc gtaaaaattg
    39721 ccgggcaaag cagcatttgc cgggtcattt ccctggaaat ttgcgggatt gcccatgatg
    39781 aacttaaaga ttactattgc tacgattaac gcaacaggga ttacaaaagt ggcgaaaccc
    39841 aagttgaact tacggctttt ggatctacga ttttttttca ttttgatggt gatttttcta
    39901 ttggtgtgat ttttgtaaat tatacgctat caagacattg tgcgcatcgg acgtggcgca
    39961 caacatgcac acgttcctga agaacatctg ttcatttact gtaaataaaa cctgaaggat
    40021 gggatcaatc tcagaccctg agagaatagc tcagactgcc gctgatggca tgagctgaag
    40081 gaatttcagt aaagcctttg gatttttggt ctttattaaa accgcatatc ggtaagcttt
    40141 gaatattgct gacaaagaaa tttgataaat tcctgtattt tgtttcactg cgtgtgtccg
    40201 acttgtactc gggaagaatg caggtaacca caacgggtac ttccgcaaaa gagcctgatg
    40261 tcccttcggg ggtcatctgg tcaacaatca ttgttgattg agccaggtca cctgaagaat
    40321 ttctggtttg agaaacctgc accctctcag ccaggttttg aatgaaagaa ggcctggaat
    40381 cagaataccc tgccaataag aaaagactta tcaacagcat tctcataccc aaggtacatt
    40441 tccttctcat ttcgaaccgc tttcgagaaa aacgcggcaa aattagaatt aattttgagc
    40501 gaaaatactt tttaacaaaa taaatatttt atcgggagct atcacgtaac atttcaattt
    40561 aacgcattga taacctttta attagacaat tttcacaagc actttgcccg gtataaaatc
    40621 catccggtgc ctgttgccat gccggcattt tatccgccgg taaaatgtgt atgaaacatc
    40681 cggatgaaca atcctgtatc aggatttact ttcttcgcct gtttcggcgt taagcggctc
    40741 aattttgaaa ttaaacgatt tgctcaaccg tcccctgagc aggtataata gtatataata
    40801 aggcacgaga atgccaagcg agagcagccc ggcatatagt tctccggtat actgtgaaga
    40861 gatcagcagg gttattaaga gtaaaacaaa gggcaaaaaa tacccgagga ttactgcctt
    40921 gttgcccata gattgtttca tgacaagatt cacatgatcg ccctggtgaa aaacatattt
    40981 gtgctcattt tttacttcaa tcagtttttc ggtcatatct gaggccgaac acatcccctt
    41041 ggccctgcat gtggcgcaag ccgacaacga gatgatctgc acactgatct tctgatcgtc
    41101 catttcaacg atcctgccct gatgggagac ttcgggtttc ataggaaaca aggtttaaga
    41161 ttgaaggcca acgtctgcat gttgccggca tacgaccggt gcgggaatac ctgcctgcgt
    41221 gtccggcaaa agtataaagt aatttggatc cctgccggtt aaatttagcc ggatacccaa
    41281 caacagcccc atgtcatccg gatgtacgga aacagatagg gtgaagcctg aaggtataaa
    41341 aatcaaaggt aatcttcaga aacattcatc attcgtacga atggcgcaac agcacggttg
    41401 taaatgccgt ggacataagc aatagccatt ccgtagttgg taaccgggat acctgcgtca
    41461 acggccggct tcagccggtt tatcagttgc tttcgcgtta tcatgcaccc cccgcattgt
    41521 atcacgatag cataatcttt gatcgcagcc ggcaactcat taagacccgc cacaaccgtg
    41581 tattcgagct ttttgccggt aaaattactc agccagcgcg gaattttaac ccggccgata
    41641 tcatcgcacg acacatgatg gctgcacgat tccagtatca ggaccttgtc gccatcctta
    41701 agtttggaga tatgcggggt acctttcaga taattcggaa aatcgccttt ctgacgggcc
    41761 agcacaatac tgaagcttgt gagcggaata tatttcggga tagaggcgtc agctttggga
    41821 aaaacctggc tgtcggtaat taccaggctc ggtttgggat ccatcttacg gatgtatgca
    41881 tccacttcgc gctccttaca aaccacggca attccatcat tatcaagtat atcgcggatt
    41941 acctgtacct gtggcaggat gagccggcct tcgggcgctt caatatctat gggggtaacc
    42001 agcaatactg tatcgccata tgcaatcagg tcgccgatca gggtattctg atttagggat
    42061 gattccggta aatgcttcct gatcaggctg atcagccttt caacctgagg attgctggtg
    42121 gaactgaaat caagaacatc cgtactcaac gaagtcctga tcagccgttg cgtttcagaa
    42181 tatatcggag caatatccgc tttattgtga acaatgaaga acggcacgtc aaattctttg
    42241 aattcgctta ccagttcctt ctcaaatgcg ccgaaggtat tatgcgtgat gatgagaatt
    42301 gccaggtcga tggttttaag tacctccctg gttttggcga cacgtttttc ccccagcaat
    42361 ccgatatcgt caattccggc cgtgtcgata agtactaccg gccccaggcc ggtaatctca
    42421 attgttttcc gcaccggatc ggtggttgtc ccggcaatat ccgaaactat agccacatcc
    42481 tgcccggtga tggcattgat gagggagctt ttcccattat tccggcggcc aaatatacca
    42541 atatgaccgc gaagtgtttt agatttcatg agtgggggtg ttgagttgct tcagagtata
    42601 tcatttagtg aataaccggt ttttaacaga ttttctggtt tcagggcgcg ctccagtatc
    42661 tctttgctta caatgttcaa ctgcttgttg gcgtcaacaa tactaatgga cttttctttc
    42721 atcagcctgg caagttcagc cgaccggtca taaccgatgt aaggcacgag cgccgtggtt
    42781 atcgaagggc tgtaaaaaag tcttgttgca gcggttcctt tccttacggc aagcccttca
    42841 aagaaatttt cgcgtaatgt cctgctgact ccaatcaata atttcaggct ttcgatcatc
    42901 gcgttgccga taacgggaat gtaggcatta agatcaagac acccctgggc gcttaacgaa
    42961 ctgatcagca tgtcattgct gtaaatctta tgcgctacgc ttatggcaaa ttcagggatc
    43021 accgggttta ttttgccggg catgatgctg cttccaacct gcctgggcgg gatgatcact
    43081 tcggcggctc cgacatccga tgctaacagc ctgaggtccg aaactatctt ctcgatattg
    43141 acggcaaagg atttcaaaat cgcatgaact tcaacgaaag aatccaggtt gttggtagca
    43201 tcgggcaaat tttctccacg ggccacaggc aatcccgtga gttgctgaag aacaggcaca
    43261 acctccatga taaaaaattg cggtacagtt atcccggtcc cgatagcgct tcctccaagg
    43321 ttaatgattt taatcctttc aaagcatttt gaaacacgcc accagtcacg ggaaagcgcc
    43381 tcgctgtatg tactgaaaag cttcccgtat gacgacggca ccgcttcctg catctgtgta
    43441 taggcaatgc gcaactcatt acggtactct ccttccaatg cttcaaccga ctgcctgagc
    43501 atattgatgc tgttttcgag ttcatcgagc aactgcatta tggctatttt cagggaagtt
    43561 ggaattacat cgttggtcga ttggaaaatg ttggcggatt cgataggatg aacgacagaa
    43621 taatcgccgg gtttatggcc cagtttgatc aaagctgcat tggcaatgat ctcatttaca
    43681 ttcatgttga tgcttgtacc tgcccctccg ctgatagcag gaactatgaa ctgtcctgaa
    43741 tacccgccgg cagccacttc ggaggcagcc tcggtcatag ccttgattac gctgtcatcg
    43801 atcagcggga aattaaagac agcatcgggg tacttcgacc ggacggctgt tttaaatttc
    43861 tggtaggtaa gatagcaagc ctgtttaacc aaaccgacag ctttatacca ttccggaggg
    43921 aatcgggtat catcgggaaa gtttgagatt gcccgaagtg aatggatacc gtaaagggca
    43981 tctgcaggaa cctcaaggga acccagggaa tccttttcgg agcgtaaatt tgttatcttt
    44041 tcattcatgt gcattcctcc tgttccaaat ttactgcttt ttgttctgtt tcttcaggct
    44101 ggttctttct ttcagcgctt tataaccgga taacttctca ttcaattgct tcatattggt
    44161 agccgtaatg gtaagtatcc cttccgggcc agcctgtacc gaaatcaggt attctttgtt
    44221 attgtcgcgc aggttgtaat taaggtaagt aatcccgcga ttactgccgg ctttgatagc
    44281 gggaaccttt ttagctttga cgagataatc attaacttcc gaaaggtgag ccactgaagc
    44341 gattgaatct gcccacattt ttgctgtatc ataggaactg atataaacca gcgtcacgga
    44401 attaaatcct tcatccggcc tgtcgaccga tgtttgttgc gaaagaacgg aatccagcat
    44461 cataccccgg gggtcaatca gcccgagttg taaagcccac cggggcaatt cataagcttt
    44521 atggagccct ccgagttgag ctgaaatatt ctcataggct ttatcataga cgattttttg
    44581 cctgatgtaa ccctgatagg ttaactttcc ggaatggtat ttcttttcca aagtcagtaa
    44641 tcctgattca acagagtcaa gcgcagcaac cagcgaatca tttttagctg cctccagccg
    44701 tttctgcctt gcagggtcgc atgcccccag cagcactaca aaggctgcca gaatacaaaa
    44761 cacccgaaaa aggcgtgcat gaagcatgat gagaaaaatc aaaaatccgg aagagaagtt
    44821 acacgaaaaa gcccacgatt ctccggcctc aataatacga aaatattatt ctactcacaa
    44881 aaaatatttc gagcaggatt tcaatgacaa tatttatttg cataaaatta aatatcaaca
    44941 cgttatgtga gctacacttt atactaagcg catttgaatt aatttagcaa gcaaataaaa
    45001 agtgatggta tgaactacag atatcttggt aattcaggaa taaaggtttc ggaaatatgt
    45061 tttggggtta tgacattcac gggggaaaaa ccgaccgaaa ccggatcaac gaatcagaaa
    45121 gaagcagatg aactgaccgg ccttgcgttg gatcacggta taaatttttt cgataccgcc
    45181 gatgtttatg ccaacggcat ttccgaaaca atactgggca aggccttggg cgtcaggcgt
    45241 aaagatgccg tagttgcaac caaggtacgt ttcgccatga gcgacaagcc caacgatacc
    45301 ggactctcgc gtcatcacat tctcgaaagc tgccatgcca gcctgcgccg cctcggcacc
    45361 gattatatcg atctatacca gatacacagc tatgatcccg gaacgccgct cgaagaaacg
    45421 atcagggcgc ttgacgacct ggtcagggca ggaaaagttc ggtatattgg ttgctcaaac
    45481 cttactgcct ggcaaaccat gaaagcgcag gctgtttccg aaaagctgaa ccttgagaaa
    45541 ttcatcacca cccagcttta ttattccatc ggggcgcggg atgtcgaaca ggaactggtt
    45601 ccgttgtgcc tcgatcaaaa attgggaatt ttatgctgga gccctctgtc aggagggttt
    45661 ttcaccggca aataccggca gggcatgcct ttgcccgaag gaacccggag gagcaaccgt
    45721 aaagcgtccg gtatgaagta ctggccggtt gatgaggcca ggggttatga aattttgggc
    45781 gtgcttgata aaagcgccct gaagaacggc aaaacaactg cccagtcagc cataaactgg
    45841 ctgctgcaaa agcctgccgt tgcctcggta atcatcggag cccggaacaa ggcccaactg
    45901 ctcgaaaaca tagcttcatc cggatggaaa ctcccggaag aggacataac ggcgctggat
    45961 gaaatcagca agccggtaat cccctacccc atctggcacc agaattattc cgacaagaga
    46021 taaaacctgc cgccgtcaga ttttagactc aacgatcttc aggatttcat tctcggcttc
    46081 aatggccttc gcctgaatct cagcgccttc ggtcaggatc ttttgcacaa aatctttatc
    46141 ttttaatcct gatgcattga tctttacatt caggaaagcg cccataacag ccgaacgggc
    46201 ggccagcgcg ccaacaccgg catctgaaac cgagttggga tttcctgtct cggccatggc
    46261 ttttatcact tccatgcttt cgaatgaggt tttcatgacc ctgaagggga tttcgatggc
    46321 ataacgcgtg gcagcctgca ttgcattctc acgcgctgcc ttttcggtat cgcttccttt
    46381 gggcaagcca agagcatcca tgatcctgtt aaaggcccgg gtatcttcgt caacaaggaa
    46441 aagcagctcg tcttttatct tctgcccttt ttcagcccag ttgctgaatt cttcccagcg
    46501 ttcatcccaa cccggcttat ggcttgaaag attggctacc atagtggcca gcgaaattcc
    46561 cagggcgccc atcgttgcag acaccgatcc gccgccggga gccggagatt cggatgccgt
    46621 ttcatcagca aaaccggcaa cggacaaatc aaccagtttt ttctgcgatt tatcaatcag
    46681 caggtattcg atgatctttt catggggatt gaaaggcttc aggtcatcaa gccccaacga
    46741 tttcacggct attttcacaa gttcagactc cgaaacccca accgaacgct gctgtttgcg
    46801 aaggaaatac tttcctgcat ccaggatggc tttgagcggt attaatccga ccagttccga
    46861 accggtcacc cggagaccgc gttcctgcgc ctttttacag gcttcctcaa aagcaacatg
    46921 aacgggagta atgctgatgt tggtcaggtt gatggagacc tgggcaatac cgtattcttc
    46981 gatgtaccaa ccaatggctt tgcaggcttt tagtgagccc ggaatccaga cttcctttcc
    47041 gtttccatct ttctttttct tgcctgtaac ggcgttacct tcacgcaccg gacgaccttt
    47101 ttcacggata tcaaacgcta cggcattggc gcggcgtgtt gaagtcgtat tcaggttgat
    47161 attataggca accaggaaat cgcgggcgcc aacggcagtt gctcccgtcg tcgccaccct
    47221 ttcgttgaac ccggcaggac cgaaatccgg tttccagtga ggatcactca acttcttcgg
    47281 taaaccttca tattcaccgg aacggttatt ggccaggtta cgccgctccg gagtaaaggc
    47341 agcgctttcg taacaataaa ccggaatccc taattcattt ccaatgcgtt cggccagttt
    47401 atgggcataa acaaccgttt cttccatcga aattccagcc accggtacca gcggacaaac
    47461 gtcggtagcg ccaaaacgcg ggtgttcacc cttgtgaaaa cgcatatcaa ttacttctga
    47521 cgctttttta acggcctgga aagcagcttc gattacggcc tccggttcac caacaaaggt
    47581 tactacggta cggttggtgg cagcgccggg atctacgtcc aggagccgga cgccttcaac
    47641 ggtttcaatc tggtcagtga tttgttttat aactgccatg ttgcgccctt cactgaaatt
    47701 cgggacacat tcaatgagtt gtttcataga ttcctgatat tcaatttaat acaagtaatt
    47761 gagtttccga tgcgcaaagt tagcaattaa gtcggaggta aacgggagaa tagaaaatgt
    47821 ttgtattcag gatcgataac gtcggtattt cagaaaataa aagcattgcc agccggcaga
    47881 acaaataaat tcagtgaagt acggccacag cctctgccac tttcctcccg gtaatgggca
    47941 taatcagtaa tatagattca gggagcctcc actattgaat aaaaaatgtt taacttcgtg
    48001 attctccggt tcaattcctt ttcacaattt aagccaacct atccgatgcc caacttgttg
    48061 aaaatttctc ttatcacaat tgccggcctg ctgttggtct atgttggcat ttcaatcatt
    48121 tccctgttag cggaaccttc gcaaaacagc aacctttacg aaactcacga cggcactgcc
    48181 gttgttatca cgggcgctgc cgcccgcatt gttcaggaag ttgctttgct cgaacaattg
    48241 caacaagccg gatggctgaa gaatgtctgt tttatttcag gggcaagttc cggggcgctc
    48301 aatacggtga tgctcaatgc aatcctgaca cataaattct cctggcagcg ataccgccaa
    48361 ctattatttg gccttaaaag cgatgatgta ttttcaaggg aaggcaactc cctcccgttt
    48421 gatacgaatc cattaaaaaa cctgttaacg agtgtaatct gcgacagcct gggttacgac
    48481 agcatcggga acctcccgtt ggccagttcc atctccgtta ctgatctgga attcatacct
    48541 ctttcgctca aaacatccag gcttagcaac cggaaaatca accaggaaag caagcctggt
    48601 ttgaacctgg tggatgtcct gatggcttcg acggcaattc ctgtcctttt tccttctgtc
    48661 aggctccgcc cttcacccac tatcccctca gccaatttta tcgatggcgg cgtatccgaa
    48721 gaccatattc cgtacagggc tgtcctgcaa ttcgaaaaat acaggaagat gaatgttgac
    48781 acactcatta tcgtaagccg gaaatgtgat acaaaactcc agatagatac tgaaatgagg
    48841 caattgggag taaaagagaa agaccggata cttctggaaa aagccggtat tcgcctggag
    48901 aacctggcta aagatggttt tatcaaacag atgaaagaat tgaaaaaatt ttacccagat
    48961 ctggccgctc atacttatgt atatataccc gattttcagc aaaacttccg gatgatggat
    49021 ttcggacgaa tggaggagca atacgacata accgcaaaat gggcagaatc tcacagtcct
    49081 gtaccgttag atgaatatct gacttctgtt gataatcagt aaattatcag cccggtagcg
    49141 cggccagaca aggtcctggt ttgtgagaga tgctcgtctg aaaaatcctc cgtcatcaga
    49201 tttaatatga attactcatc ctttccggta gtcccaaaaa aaggattcgc taattttatg
    49261 gcatcaaatt tctccgaata atcttcaatt accatgcaca atctcctgaa aatcaccctt
    49321 actgcagtat ttgcaatact gctatcgcaa acttatgccg gaagaaaaga aaggaccata
    49381 aagactacat cgcccgacgg acatatcgtt ataagcctga caatagccga tatgccggtt
    49441 attacggtta cctgtaacgg ccaggaaatc atgtcgcctt caaaacaggg cctggcgatt
    49501 cgtgagcatc cggaagccta ccttaacccg attgtaaaaa aagtgaaata ccgggctgtt
    49561 gatgaagtaa ttgtacctgc tgttgcagaa aagcgccaac agattcctga tatttataat
    49621 gaaaccgaaa tatggttcag aagcaattgc ggcattaagc tccgcgcata caacgatggc
    49681 gccgcctggc ggttcatcac gcggttcagc gacagtgtta cggttaacca ggaacatgtc
    49741 accttgagaa tggccgcatc cgattcggtt tacttcggtg aggaggatag tttcctgtcg
    49801 catagcgaac gtctctaccc tttaaaatca gcctcagaaa tcaagggcaa acaaatgtgc
    49861 gtgatccctg ccgttttccg taaaagtaca gggatcattg cagcattcac cgaagccgac
    49921 ctgctcgatt accccggcct ctaccttaca ggcacttccg aaggttctgc cgtattcaaa
    49981 ggaatccagc cgcaggcggt tgctacggag tcggttagta aggataagcg gagttatgtt
    50041 gtggatacaa ggcatgatta tattgcccgt actgccggtt cgcgcgaata cccctggagg
    50101 gttttcggga ttgccacaaa cgacgctcaa ctgctcggaa acgatatcgt ttaccgcctc
    50161 ggaagcccct gtaaactcac cgaaaccgac tggataaaac cgggaaaagt ggcctgggac
    50221 tggtggaacg actggaatat ttatgatgtg cctttcaaag ccggcatcaa taccgaaacg
    50281 tataagtatt acattgattt tgcttcgaaa aacaagctgg aatatgtgat tttcgacgaa
    50341 ggctggtcgg ccaatgacga ccttgataag ataaatccgg acatggatat ggaggccctt
    50401 tttgcttacg cgaaagagaa gaatgttaaa atcatccttt gggtcacagg acgtgcatta
    50461 caggacaagt tcacttcctc gctcgataag tttgaaaaat ggggttgtgc aggcattaag
    50521 atggatttca tgatccgcga cgaccagcag atggtgaatt tctatgaaaa agtagccgcc
    50581 gaaactgcca ggaggcatat gcttgccgac ttccacggtt cctttaaacc taccggacta
    50641 agccgcacct acccaaacca acttacccgt gaaggcgtcc gcggactcga aaactcgaag
    50701 tggagcaatg aaattacacc ggcccatgac tgtacccttc cgtttacccg gatgtttgcc
    50761 ggccccatgg attacacgcc cggagccatg accaacgcaa cgaagaagaa ttttgcaatt
    50821 tcctggagcg agcccatgag ccagggtacg cgttgccacg aactggccaa gtatgtcata
    50881 tttgaaagcc cgctccagat gctgtgcgat gcgccgacaa attacatggg cgagacggaa
    50941 gccatgaatt tcctgtcccc tgttccgact acatgggatg aaaccatagg actgggtgcc
    51001 aggatcggac aatatgtagc tgccgcccga cgccatggcg acacctggta tatcggcgct
    51061 atgaccgact ggtctgcccg cagtctcgaa ataggccttt catttctggg cgaaggaact
    51121 tttaagctcg aaacctggta tgacggcccg aacgccgacc ggaaaggtga agattttctg
    51181 accggtaata aaattgtcga caagactacg aatctgaaag ttgaaatggc tcccggcgga
    51241 ggttatgtag cccggattac caaaatgtaa tttttttgtc ccctatgacg caaagacaga
    51301 ccggataccg atacaggcat gcaatcatgc tcaggttttt gataatgttg ctaacggtca
    51361 gtggtattgc cgaatgcagt ttcgcccaac agaatattga acaacacatg atgctgttgc
    51421 cgcaaaagcc agcatctgtt tccggccccg actggctgct ggacggcagt gcatacaaag
    51481 ccggcgttta tcagggccaa agcccaaaag acctggtcat cagcaacggc ctggtcgaaa
    51541 ggacatttcg catttctccg tcactggcat gttacagttt ccggaatctc attacaggcc
    51601 aggaaatgat aagggccgtg aaacccgaag ccatgctgac catcggaggt aacatagtgg
    51661 aagcaggcgg cctgtccggc cagttcgaat acggatatct caaaaccgag tggctaaatt
    51721 ctttcaccgc ttccgggtca tttaccctga ccgatttttc ggtgaatgat ataaagcctt
    51781 tccttgaatg gcagcctgtc aggtggatac cggatcatac ctggccgccc aaaggaaagg
    51841 aaatcacctt ttatcttgaa ggatcatcgc ctgtcacaac agggattgag atcgcaatcc
    51901 attacgagat ttatgacggg ttccctttgg tttcgaaatg gctggaagtt gtaaacaacc
    51961 gccctgaggc tatcagcgtg gatagagtaa ccagcgaagt acttgccttt gtggaagaag
    52021 aagtatcggt tgacaaaatg aaccagtgga aaacgccgaa catcttcatc gccagcaatt
    52081 acagcttcat gggaatgaca ggtccttcag ccgaccgggt cacatcctgg cttgccgaca
    52141 gcgcttatac cacgcaggtc aattacgaac gcaaaacgcc ctgcctgctt gtctgccgcc
    52201 ctcctctcgg ccccggcatc aaactcgcct accagcaaca ttttacttcg ctgagaatct
    52261 gggaactttg ttacgactct tacgaccgtg agcgaaaagg gttatccgaa aggaaaatgt
    52321 acaggaccat ttctccctgg gcgctggaga acccgatctt tatgcacctc acatcctccg
    52381 atccggacgc gataaagcaa gccgttgacc aatgcaggca aacggggttc gaaatgatca
    52441 ttctcagttt cggcagcggg ctgaatatgg aaagtaccga taccgcgtac atcaggaaat
    52501 tcagggatct gaacgcctat tgccgcaaca gcggaatcga actcggaggt tattcattac
    52561 ttgccagccg gaggatcaac gatgaggaag acgttattaa ccccgttacc ggaaaaaccg
    52621 gcggggctgt cttcgggaac tccccctgcc tgggaagcaa ctggggaaac cgttattttg
    52681 aaaacatccg gcatttcatc gggaacacag gcttttcgct tcttgaacac gacggttctt
    52741 accccggcga tgtgtgtgcc tcaacgaaac atcccggtca cgctgggctt gaagattccc
    52801 aatggaggca atgggagaaa atcaccggat tttacgcctg gtgcagaagc cgggatattt
    52861 atcttaatgt tcctgactgg tatttcctgg ccggttccaa taaaacaggc ataggttacc
    52921 gcgaaaccaa ctggtcgctt ccccgggaaa ggcaactcgt actgggaaga caaaaccttt
    52981 acgacgggac ctggggcaaa acgccatcca tgggatggac ttttgttccc cttcaggaat
    53041 accagggagg cggtgaagca gccacccttg agcctttgtc gcaacacctg aaggaatacg
    53101 aagcccatct gttccagaat ttcggggccg gagtacaggc atgttatcgc ggttcaaggc
    53161 tttttgatac cgaagaaaca cgtcaactgg tagccggatg ggttgactgg tacaaaaaat
    53221 accggctgat cctcaactcc gatatcattc acctccgccg cgccgatggc cgtgattggg
    53281 atggctttat gcacgtcaac cccgcgctga aacaaaaagc cataatcctt cttttcaatc
    53341 cgctaaagca accggttgaa cgcaccgtta ccattccctt atattattcg ggacttaccg
    53401 ggaaagcctc catcagggaa aaagaagggc ccggcacggt tgtccttctg aattctgcgt
    53461 gcgaagccaa catctccgta aaattgcctg ccgaaggttt cacatggctg gtcgtggaat
    53521 aaaccccctc tcccttcggg gtttttccgg caccagaaat tctgcctatt tcaacaattg
    53581 cgacaccttt ccggcaacag gaattctgtt cgtaaattta tcccggtcag gaaaagggaa
    53641 gctgcggcat gaatccctgc tgatatatca gtttagattg ataataattt agggcaaacc
    53701 gatcaatgaa gcgccaatta ttgtaaactt gcgacccaac aacaaaacct ttttcagaaa
    53761 tgcctattgt aaccgatagt cacctgcgtg cctttgttga tgccgctcac cgtatcggca
    53821 ctcatcaatt gttactctgc agcagcggta acctttcatg gcggatcaac gaacagacaa
    53881 tattgatctc ccaaaccgga agctggcttc catacatgac caaagaccag atctgcatcg
    53941 ccaacatgtc ggacggtgta gtaatcaata atgtaaagcc ctcaatggac agcggatttc
    54001 atttcggagt gctgcgtgca aggaaagatg tcaatgtagt ccttcatttc cagtcacttt
    54061 acgggacaac gctggcaagt ctccccaatc cgccaatcaa cttcaatgtg attaacgaag
    54121 tacctcagtt tatcgggccc atcgccatgc tggattatat ctgcccgggg tctccggaaa
    54181 tcgggaggat ggtgaccgac accatgcgaa acaacaacct gctggttctt cgaaaccatg
    54241 gccaggttgt cgtcggcaaa gattttgatg aagtcatcca gcgggccctt ttctttgaac
    54301 ttgcctgcgg catcatcatg cgcagcaata atgaagccgt ccgtctgacc gaagaccaga
    54361 tctgtgaaat gaaccggtac atgaaaaagt agtcaggatt cactcccggt gagttttcaa
    54421 caccataccc ttcatccaaa acaacgaaac agacccatcc actcgtaatt tattgatatg
    54481 aaccggctta aaggatcatt ccccctggtt ggaatccgcc ccgttattga cggccgacgg
    54541 aaaggtgtgc gcgaatcgct cgaagagcaa acaatgaaca tggcccgcca tgccgcggaa
    54601 cttatcagtt caaccctgca ttatcccgat ggctcccctg ttaaatgcat catagccgat
    54661 acctgcatcg ggggtgtagc cgaagcggcg gaatgcgccg aaaagtttgc caggaacggc
    54721 gcaggagtgt ccattacggt tactccctgc tggtgctacg gtaccgaagt catggatacg
    54781 gatcccctta ttccaaaagc ggtgtgggga tttaacggca ccgaaaggcc gggagccgtt
    54841 tacctgacgg ctgcgcttgc cggttatgcc caaaagggcc tgccagcttt cggaatttac
    54901 ggacaggatg tacaggatgc cgatgaccag tcgataccgg ccgacgtcag ggaaaagata
    54961 atccggttta ccagggctgc ccttgcggcc gcctatatga agggaaaatc atacctctcg
    55021 ctcggatcgg cagcaatggg aattgccgga tccattgtag atccgtcatt tttctccgac
    55081 tacctcggaa tgcgcaacga gtatgtcgat atgagcgaat tcatacgccg catcgaggaa
    55141 ggcatttatg accataatga gtttgaaaaa gcattagact gggcaaaagc caattgcctt
    55201 gaagggccgg atatgaatgc ccgtccgttt ccccaggatc aaaaagaaaa aacctgggaa
    55261 tttgtcgtaa aaatgaccct gattgcccgc gatttaatga tcggcaatcc taaactggta
    55321 agactcggtt ttggggagga agccctgggg cataacgcca tcctggcagg tttccagggt
    55381 cagcgccaat ggaccgacca ttttccgaac ggcgatttca tggaagccat ccttaacagt
    55441 tcctttgact ggaacggcat acgccaggtc ttcctgatgg ctaccgaaaa tgatgcattt
    55501 aacggagtcg ctatgctgtt tgggcacctt ttatccaata ccgcccaaat gttcagcgat
    55561 gtgcgcacct attggagtcc cgaagccgtg aagcgcgtca ccggaaaatc actggaaggc
    55621 caggcagcca acggcattat ccatcttatt aattctggcg cctcagccct ggattttacc
    55681 gggcaacaga caattgacgg aaaagccgcc atcaagccat tctggaaaat tactgaagag
    55741 gaagcaaacc gttgccttaa aaacacgcgg tggccttcgg cgatttacga atatttccgc
    55801 ggcggcggat tctccagcga attttcatcc cgcgggggaa tgcccgtcac tatgagcagg
    55861 atcaatctga taaaaggcca gggacctgtg ttgcaactgg ccgaaggctg gactgttgaa
    55921 ttaccggccg acgttcatca aatccttacc agacgcaccg atcctacctg gccgacaaca
    55981 tggtttgccc cccgcctgac ggggagcggc gctttcaccg atgtttattc ggttatggcc
    56041 aactgggggg ccaatcacgg ggccatcagt ttcgggcata tcggcgccga cctcatagct
    56101 ctagcctcca ttctgcgtat cccggtgtgc atgcataata tcaaggcttc ggcaattttc
    56161 cgccccgcgg cctggaacgc cttcggtact gccgatcccg aaggagccga ttaccgggcc
    56221 tgtaaaaatt tcgggcctct ttacggaaga taaaacatca acctcaacgg agcaacatgg
    56281 ataaagctgt tcttgtgctg gattgcgggg ctaccaatgt tcgtgctgtt gcagtaggcg
    56341 caaacggcag cctgctagca tcccgttcat tgcctaacat ggtccgccct gatcctgatt
    56401 ttgaaggcgg acttatctgg gatgtggaag aaatctggca aaaattatgt gcatgttccc
    56461 gtcatgtgat ttccagcctt caagataccg ccatcggagc agtgacggca acgactttcg
    56521 gggtcgacgg ggcgccgatg ctgaaaaacg gcacgctctg ccatcccgtc atttcctggc
    56581 aatgcagccg tactgtcccg gtcatgaatg ccattgggaa atacatcgat cctgcggggc
    56641 tttcggcgat caccggctta caaccatatc attataatac gatcaataaa ctggtctggt
    56701 ttcgcgaaaa ccgctgggat gtcctgaggg tgatgaaatc ctatgtgatg atgccttcca
    56761 ttttcctgca cagaatgacc ggggaattcg tcaccgatac gaccatggcc ggcacgacga
    56821 tgctgacgaa tgtaaagagc aggaaatttt cggatattat cctggaagcc atcggcatcg
    56881 gcaccaaaat atttcctccc ctggtggaac ctggcacggt ggtcgggaag ataagggcat
    56941 cagcagccaa agacctgggt attgatcccg gaattcccgt tgtagcgaca gggcacgaca
    57001 cccagtttgc cgtcttcgga tcgggagcgg gcatcaacga accggtgctc agctccggta
    57061 catgggaaat tctgatgtcg cggactcctg ccgaatcaat caacattcct ccggtttcgg
    57121 aaggagtcac ctgcgaattt gatgcagtgc ccggccttat cgacctcggg gtacaatggg
    57181 tagcttcggg cgtgctggaa tggatcaccg cattattcta cggcgaatac aaggacagcc
    57241 cttcccgtta ccagttgatg attgaagaag cgcaggccat tcctgccggg tgttgcggcg
    57301 tgaccatgtt gccggaattg ttcgacggcg gttttatccc ttccaaaggg tgcatcaacg
    57361 gactaaggca tgacacttcg cgggcgcaaa tatacagggc tgccctcgaa gccctggctt
    57421 actacacgcg gacaggcctg gaaatactaa agaaagccgg cgcgtttaca cccggaagcc
    57481 tgctcggcgt tggcggcggg acgaaaaacc gcctctggaa ccagattcgc gccgacgttc
    57541 tgcaaatgcc cttcaaggtt tccaaccgca gtgaaaccac cgtgctcgga gcagcgcttt
    57601 ttgccatgcc ggccgcaggc ttgtaccatt ctgttgaaga agctgtgctc tcggttgaga
    57661 atcgggtcga aacattcctg cccggcgccg gcaccggcgc atatcagcaa ttgtattcag
    57721 atttccgggc caactggttg ccggaaagta atttatgaac cctatcgatt cgttatgaag
    57781 aataaaaaag tatttgtcac cggttgtttt gatatgcttc acagcggcca tgtcgctttc
    57841 ctgaaagagg cttccggtta tggagaatta tatgtatgca tcggctccga taatacgatt
    57901 cacaacctga aaggccgtta taccgtgaat tcccaggacg aacggagata tatgatcgaa
    57961 gccctgaaat gcgttaaaga gtgccgcatc aactccggaa gcggcatcat tgattttgaa
    58021 aatgaattgc acgatatccg cccggatatt ttcattgtga atgaagacgg ccatacaccc
    58081 gccaaggaag cactgtgcag cgaactcggg attgagtaca gagtgctgaa aaggattccc
    58141 tatgccgatc tgcctgtccg ttctacaacg gcgctgcgca acgtttgccg cattccttac
    58201 cgcattgacc ttgccggcgg ctggctcgac caacctttcg tttcgaaata tcatcccggc
    58261 gctgtgctta ccatcagcat cgaacctacc gttgaattta acaaccggtc ggggatggca
    58321 tccagcacgc gccgaaaagc catcgaactc tggcgtaccg aaatccccca tggcgatctg
    58381 gatcaactgg cccgtatgct tttcagtttt gagaatcctc ccggaaccat tgaaattgcc
    58441 ggatcgcagg attcactggg cattgtttac cccggcctta accgtcatta ttacgaaagc
    58501 aattactggc cggtaagcac cgaaagcatc caggatgaag aaatcctgtc atggctcgaa
    58561 aaccaccttt acctggtaac ccttggcccg cgggtgagcg aatacacggt tatcgataac
    58621 accaacatca gcacggatgg tgcaaaatcg cttgccgctg ctgccgatgc ctgctgggat
    58681 gccattttga gaaaggatgt caacgccttt ggctcggcat tccgtcaatc gttcgaagcg
    58741 caggttgcta tgttcccgaa tatggccggc cgggagattt tcgaaattat tgatcagtac
    58801 aagcacctgg ccaaagggtg gaaactttcg ggcgccggcg gcggaggtta cctcatcatg
    58861 gtcgccgacc ggccggttga cgatgccatg cagatcagga tcaggagaca aaacgggctt
    58921 tgacaattct tcgggcaaaa caggataaac caatactaac tcgtattatg ttttatgaaa
    58981 cacaaactga ccaccaaaga cttcattttg ccatttatca tgatcacctc cctgtttttc
    59041 ctctggggaa ttgcacacgg gatggtagat accctggata aacattttca acaaatactc
    59101 caccttaaaa aatggcaatc aagttttatc cagttctccc tttacggcgc atatttcacc
    59161 atggcgttgc cggcaggcta tttcatgaga aaattcggtt ataaaaaagg aattatcgcc
    59221 ggattgtccc tttttgcggc aggggctttt ctgattgccg gaacagccac ttttgaatca
    59281 ttctggctct tcctgatctg tctgtttatc ctgggatgcg gacttgccac actcgaaacg
    59341 gctgccaatc cttacactac caaactgggt ccccctgaat cggccgaaca gcgaataaat
    59401 ttttcgcaat cgttcaacgg gctggcctgg gtgctggggc cgcttatcgc cctgagcatt
    59461 tacgggaacc agagcgaagc ggaaggtgag aaaatgctgt cgctgatcat ccctatggga
    59521 atcatcgggc tggtggtgct tttatttgcg ttgatattca tgcgccttcc gcttcccgaa
    59581 atatccgaag aagatgcctc cattgctcat gggacccatg tttccggagt tgaagaaaaa
    59641 ccctcgtttt ttatcccgtt gagcaagcag ccacatttta ttttcggcgt aatagcacag
    59701 ttctgttatg ttgcagccca gacaggtatt ttcagctatc tgatcaactt cgtaaccgat
    59761 gaaggccagt atccgcaatt tgatgtaaaa tatgcccctt actttttatc cataggcttt
    59821 gcccttttca tgctcggacg catggcagga agttacctga tgggtatcac caaacctacc
    59881 cgcatcctgg ccatccacgc cgctctctgc tgcctgctgc tgcctgtggt aagttcccat
    59941 ttgggttgga tatcactgat atcgctctat gccatcttct tcctgatgtc aatcatgttc
    60001 ccgacgattt tcgccctggg cattaaagat ttagggccta aaaccaagaa ggcatcttca
    60061 ttcctggtca tggccattgt aggcggcgcc atatttcccc cgtcgatggg ttatgtctct
    60121 gatctgtacg gcatgtcaac gggatttctt gttccgatcc cgttgtttgc tttcatcctc
    60181 ttctatgccc tgaaagggca tcaggttaaa gcctgctgac acctttgccg gcacaaattc
    60241 catttgttaa taattactgt tcctttaaaa acaatcgacc atgaaaagag cgctaatgat
    60301 cattatcctg tttgccgttg cagcaggtta ccttcccgcc caggaacatg aatcgccgta
    60361 tatcccgcct gccgaccccc tggttttaaa aaacctggaa gaatggcagg atatgaaatt
    60421 cgggatattt atgcactggg gaacatacag ccaatgggga attgtggaat catggagcct
    60481 gtgccccgaa gatgaaggct ggacccaacg caaaggcgaa ttttccgaca actatttcag
    60541 gtataaaatg gcttatgaaa acctccagac ttcgtttaat ccggttgact tcaatcctga
    60601 aaaatgggcc gcagcagcca aagaagccgg ttttaaatat gtggttttca cctttaagca
    60661 tcacgatgga ttttgcatgt tcgacacaag gttgacagat tacaaaataa cttcaccgaa
    60721 ggctcccttt cattccaatc cgaaagccaa tgtcgggctc gaagtttgca aggcgttccg
    60781 gaatgaaggc tttaaagtcg gcgcctattt ctcaaaaccc gactggcact gccccgatta
    60841 ctggtggcca tattttccgc ccaaagaccg caatgtgaat tatgatccgg cccggtaccc
    60901 ggatcggtgg aatgctttta aggaatttac gtacaaacaa attgaagaga tactcaccgg
    60961 ctacggcaaa atcgacatcc tgtggctcga cggaggatgg gtacgcccaa tgagcaccgt
    61021 agataaaaat gtggaatggc aacgcggtat cgtttacgac caggatatcg atatggcgtc
    61081 catcgcaaaa atgggcagga agaaccagcc cggactgatc gtggtggacc gctccgtagg
    61141 tacggaattt gaaaattatc gcactcccga acagcaaatt cctgataaac cgctcggtta
    61201 tccctgggaa acctgcatga cgatgggcaa ttcatggtcg tatgtgccgg gcgatactta
    61261 caaatccgtt tggaccatcg tgcataacct cgtggatatc gtggctaaag gtggaaatta
    61321 tctgcttaat atcggtccaa gccctctggg cgattacgat cctgttgcct acgaccgcct
    61381 gaaaggtatc ggcgaatgga tgaaagtgaa tggggatgct atttatgcca cacgtcccgt
    61441 agcaccttac aaagaaggga acatttgctt cacccggacg aaaaacggag ccgtaaacgc
    61501 gatttacctg gctgccgaaa acgagaaaat cctcccggaa accattgtaa tcaaaggcat
    61561 tacgccaaag aaaaagaccg atattacact tctcggcgca aaaggaaacc tgaagtggaa
    61621 gacggtgaat ggcataacca ctatagcaat tcctgataag ctgcgaaaga atcctccttg
    61681 ccggtatgcc tggacgttcc gcatcagcga agtggatcag aaataaagga ggtaacgcca
    61741 ggaaacgagg taatagataa gacctgtcag catccgccgc gggcggatct gacagcgtaa
    61801 aatcagattc actaaattcg tcattgacct tataaacagg tatcgcattt gagaagtcgt
    61861 ctgttataac caggattctc tgatagggcg gctaatgtca gcccaataaa aaacccggca
    61921 ttttggccgg gcttaatatt tccttttcag cgaactattc ttttccttgt tcctgttcct
    61981 ctttattaac atcgcctttc tcaacgctga gtaattccac ttcaaaaatc agggctgaac
    62041 ctgctggaat cggaccctgt ttctggttgc catatccaag atcggcagga atataaagca
    62101 tataaacagc gcctggcttc atcagcttca caccttccga ccatccggga attacgccgt
    62161 taaccgggaa tttggcgggt tggccacgat cacgcgaaga gtcaaaaaca gtaccgtcga
    62221 ggagtttgcc gacataatga acttcaacga tatcagtatc ggtgggcggt tcaccttcac
    62281 cgggttttat aatggcatat tgcaatccgc tgggtgtttc caccacgccg ggcttggttt
    62341 tattctcggc aaagaacttg gcattggcct ccagcgtttg ggtaagttcg gcggcttcgc
    62401 gatcctgacg cgctttcatc atcttttggt tccaggactg gaaaatctgc atgatttgtt
    62461 cctgtgtaaa aagagaatcg gcattgtttg ttgcatcctt tatgccaagg ttgagcatat
    62521 cataattgat ctcaatatta ttcttcttga aactgcggat aatatccgtt cctatgatgt
    62581 aactcaccgt gtcggtttca ttcttcattt gcggaagggt tacagcagct tcttcttttg
    62641 ctttcctgct ttttttatct tttttctggg caaatgccga agttccgcct gccagtatgg
    62701 ccagcataag tataaaaacg gttgtttttt tcatcttgac ttttttatag tgcgcaaatg
    62761 tagtgtttta cggcaactga aacgttaaga attgtgaaaa gttacggtca catggggggt
    62821 aaggcatttt ggtccgggca gcattcaccc gcaggccctt gagatttgta tagctcagtc
    62881 aggaggagcg aaattcacac cggagatggc gccagccagt aatattttac agcaccatca
    62941 tcttcatcgt cattttccgg ctcccggcgg ttaccgtata aaaataaacg cctgccgcca
    63001 atcctgttgc atcaatgatt agagtgtgaa caccggcgga agccgtaccg taatctttgt
    63061 tccatttact acagcctgtc aatgttgtaa cttccaggct tacactcgcc ggttttgtca
    63121 gcgtgatctc tgcctgtgtt tttccagtga aaggattagg ccggttctgt gataccagca
    63181 gttttgcttc agcgggttct ccggttccgg gaacatatcc taattcagaa ctgcagataa
    63241 tataattttc gctcgggtca gttggtgatt gcaggcccgg taactggtcg cgctggtaaa
    63301 tcaaataata ggaggcggga caactcagag atggttgctg acatacaccg ggatagacac
    63361 attctgcatt gttatcagga tcaaagggag cggtaatatc aacaggagat gaaaattcgc
    63421 tgaaattacc agtagttttg atcacccaga tatgtctgta atccatcccc atataattgc
    63481 aattttcggt aacggcagac catgccagta taccgaaacc cgaaccatca accttagcct
    63541 gcggcatgct cacagcgccg acattataat cgccgcaatc gcccaataca tcccactgac
    63601 cgtttccatc aacatcaacc ggggatgcaa ccagcaggtt ttgattagag agcgtgtccg
    63661 gattaaggca gccggcaaga tactgatcgt cgccttccca aaccggcata tcttcgttcc
    63721 agtatgctat tcccgactgg taaggaaaca ggtcaccggc ggcattggaa agaacgacac
    63781 caaaaaataa atgaacttta ttacaaaaat caattacaac cgacactgcc ccgtcaggtt
    63841 tataaattac atctcccgcc tggccgggat aatgccatac aacggtcttc tgccaggtat
    63901 cgccattgtc gcctgacttc atcagaatca gatcatcggc ggcgttgctc acagcaaagg
    63961 caagggtatt gtatcgcggt tctgccaggc aataccggtc ggcagtccaa cccggataaa
    64021 aggctgaatc cagccccgga atcacctcgt tccggtccca gcttataccg ccatcgagcg
    64081 aacgggaata aataagcgct ccgtcctgac catggtacaa ggctccgcta ttgtccaccg
    64141 gggtagtcag cgaaatgata tgaacctgat ttttatcaga accgcctgta accatgcgag
    64201 gccagagcaa accgggagca ttctccggca atggaatggt tgactgcatc cagtccccgg
    64261 ttccttttat ttcccgtttc aacatgaaaa ggggcgccgg cgatccggat ttatgcgaaa
    64321 caacaatttc accatttgtt ccaagccggg catgcgacgg ccacccggcc cgctgatctt
    64381 ccaggcgctc agggtcaatg ttgtaacccc agtcataggt ataataataa ttatagccgg
    64441 taccacggtc actcccgctt gcatcaattc cccttgtcca cacagtagcc atcgtttcgt
    64501 cgggaaaaag cgttaatcgt cccgaaggag cgctgcagtt cgattgtaag tcgtaccgcg
    64561 tgtgcccgat ggtatcgcag gcatactggc tgaaagcgtt gacatacctg ttttccgaag
    64621 atgtgaaggg attattttca ccattcgcca aaacaggttt ggccttaaat actttctgac
    64681 aagcattttc agggattaca ggcatttttt gctgggcaaa agccggggct attgcacaaa
    64741 aagcaaaaag ggtaaagagg tgtttcataa tagaattttt aatttcgtta atacgatttt
    64801 tgataaccgg tttatgttta ctgatagaca taagatttat tctttaacaa acttcccgac
    64861 ttccaccgtt ttctcgttac tcaggcggac aaaataaagg ccgggaggga gagttgagat
    64921 gtcgatttgc tttttattgc cattcaggta acactcaagc tgcatttttc ctgtagcatt
    64981 cagaatggtg agcaccccct catcactgga gaaatcagtt gacacttcaa ctcccaggac
    65041 agaggttgcc gggttgggat aaagcataaa acctgtcccg gcagattcat ttattccgat
    65101 atcattgata acgctaaggt tcatggaatc gcgcacggag cagagttctt cggtgacttc
    65161 accccagatt gtatattgcc ctgaagcaaa atggttaccc ggaaacgtaa ttgtacttgt
    65221 ggtgtcgcca ttcgaccaaa gatatcgccc gaaaccttct ggcatcgaaa gggttagaat
    65281 gtggcttcgt ttgatggttg tatcgggacc tatgttgaaa acaggaggtt gttggatggt
    65341 tacatgtacg gaatcacttt gccagcaatt tccggcactg acgacggtaa gccggtacat
    65401 accgctctgt gtcgtgtaaa agtccttgcc tggcgtaagt gtaccgttcc aataatacct
    65461 gacccatccg gcaggaccgg aaattgtata atcatggcaa gaaatcgtat ctggacccag
    65521 gtcgaaagca tactcgttca gatcgaggta accaataaac aggtcattgg tggaccaaga
    65581 ttgcctggag gttatcgtat cgttaccata aattccattg ccgataaacc aacctgttat
    65641 gatgatgttt ccgcaattat ccgttcctat gccggccatc cgagaatgat aatcggtatt
    65701 agataatatt gttccggcaa gcatgccatt gtcttcgagt cgtcctacta aattctcata
    65761 ccagtaatcg atgtgaaagg ttgtgtcacc tgtttttaac ctgttctgaa aactgatacc
    65821 aaaaaacagt tgattgttgc ttaacgtgat aagcaggtcg tgatttatct ggatatgcgg
    65881 agatcggaag gtcctgatcc attcaggttc ctgttcgtta ttgattttca caacagcgct
    65941 ggcaatggag tcgcctgagt tggtcacagt aacgcctccg actgtaatcg aatcagagat
    66001 attggccgtg aatataaatg ctccgtcttc atcaaccacg gtttgtaaag tgtagacatc
    66061 ccagtctttt atccatcctt tataaaaact accatccgta tttaaaccaa ttaaaaacgg
    66121 gagactatga tacatagaat tagccggaca ctgaattgta tcgtttccta ctataatgtt
    66181 ggcgggataa taatttgtat ggccagtaac atatagcctt ccatcgcccc cctgtaaaaa
    66241 attcctcagg ttgaagccac tattttcgat atgagttatc catgaaatat tagccccggt
    66301 atcgacctgc atcattgaac aaaccggttt gatgctgtga acggtgtctt gcccaaggat
    66361 atacgcattg aaaggcatat ttccatttgc catatgttca catgtcaggt aaatagaatt
    66421 tccgtttgcc tttaatccaa gcagccggtt ctgagtatca ccgccaatca gttttaccca
    66481 ttgaagccgg agatcaggac tgaattttgc aagaaaaaca tttgaaaata caaatccctg
    66541 atgtggcaat ggtagtatgg tagtatcttc tatatatagg gaatcgcaaa aatctcctcc
    66601 tacatatacg tatccttcag catctgttac cgccgtcacc gaccaaaggc catccagaat
    66661 cggaccgcgc aagtcgatat atcccttgca atccccttta cggtcgtatt ttgcaatgag
    66721 ttggtgcctt gtataccctt cgtaaccagg gtgaaaaaaa acggtatcat cgataaaaag
    66781 tgaatcgcag taggtaatac cggtaaaaat gttgttctca agatcggttg tcaagatgga
    66841 ggataaatta tacttgctgt aaccgtaatt ctgggctgcc cagcgccatc gccattcctg
    66901 ggcatcggca cagaatgtag ccgcaaaaag cgacaacccg atcaaggctg aaataattaa
    66961 aggtttcatg gtatgaatat ttatggttgc tttataaact aatttttaat aaattttcaa
    67021 tttccaccgt tttctcgtta ctcaggcgga caaaataaag gccggaaacg taatccattc
    67081 aggcatacaa tcttgtataa aagcaggatt ccttgttatg atcatcgcca cgaacagaag
    67141 catcagattt ttcatacgat atgcctgaat ccaataataa atataaacgt tatcctttat
    67201 cctgaaaatc gcctcatcaa atataatttt ttttcgaatg taatttattt gttgtctcaa
    67261 aagaaatcaa caaataaata aaccgatacc tacctaacca tgcagtattt tttgtatttt
    67321 agaaaagcca tttcaatcat acttctttca tgaattctat taccgacttt caatacgatc
    67381 tgatccctac gcttttcacc aaaagactta tcctcaggcc ttttgcctta accgacggcc
    67441 ccacagtgag ggaacttgcc ggaaatatta aaattgccga aaacacgctg aacatgccgt
    67501 acccataccc cgaaggtgag gccgaaaaat ggattgccac ccacgccgcc gagttttccg
    67561 ggaaaaggaa cctggtgctt gccatctgta aaaaagacga cagcaccctg gtaggatgca
    67621 tcggtttaca actgaacctc tatgtcggca atgccgaact cggctactgg atcggcgaac
    67681 catactggaa aaacggttat gctaccgaag cagccagaga gatgatacgt ttcggatttg
    67741 aaaaactggg gctgcacaag gtctttgccc acgtttatgt caccaatcca gcctctgcca
    67801 aagtactccg gaaagcaggc atgagacagg aaggatacct ggaaaagcac gtccgccact
    67861 ggggcaatta caaggacctg ctcattttcg gaatcgcctc cgtataaatg cgcagatgtc
    67921 ttttattcca ccttttatac atgattttat cagcaatcca ggaaatttct caaccaaaga
    67981 aaacgcttgc catgaaaaag ccactgtact ttctcctgct tctggccata accctggcct
    68041 cctgcacgaa cctgaagacc gtcagggtaa aaaaaacgaa ttttgacccc gaaacacagc
    68101 ggggacaaaa tctcgtgttt acgttcagcc atgaactggt ttccgattcc ggattaatga
    68161 ataaatggga tactaccact tatatcaagt ttgaaccgaa aatcccgggc aaattcatgt
    68221 ggacaggtaa atcggaactt acattttcgc cttcacagtt gctggcgcca agcaccgatt
    68281 tcaaagctac tttaaccaat gccatcctga aatacgataa aaagaagcta tctgtcgatg
    68341 atgaggcgct tccttttcat acgccttata tctcgctcga atcggtaagc agttactggg
    68401 ctttgggcag cgatcccgca gccaacatcg aagtaagatt gctgctttca ttcaatggcc
    68461 cggtttctgc tgcctcgctt gcctcgctag ctactctgga agttaagggc aaatcacgcc
    68521 agtttcgtgt gatcacccaa aacgattccg acgttatcga gctggctttt gccgacgaaa
    68581 cacagtcgcc cgacgaggta accgaaggca aaatcacaat cggaaaagga ttctcctgtt
    68641 caggcagcaa ttataaaact acggatgcga tcgtcatgac attcacggtc ccttcgaaag
    68701 acaaacttac cattaccgac atctccagcg attttgaaga aggcgaaggc gttatttcca
    68761 tatttactac acaacccgtc attgatgaag ggctttcttc cctcatttcc gtgcagccgg
    68821 ccgtttcgtt caccaccgaa attttgccga acggcgtgaa actgaaaggc ggcttcctgg
    68881 caaatgaagc atatacggtt aaggtctcac cagccctaaa aagcgttttc gggcgggaac
    68941 tcggtgaaga atttatccaa accgtggctt tcggttctcc ggagccctat gtttcgttca
    69001 ccgaaaaaca cgctctttac ctctccacgc gcgggtcacg gaaccttggc gttaacatta
    69061 tcaatatccc cgaagtgagg gtaagtgttt tcaaggttta cgaaaacaac atccagcatt
    69121 acactaaact gggacctgag tggaactggc agtatgatga gaataatgat gagtaccatg
    69181 agtacatgac gtacgccttt gatgaagatt acgggagaca ggtgatgagc aaggtgatta
    69241 aaacccgttc gttgccaaaa tcgggcaaca tctccctgct caacatcgac cttgccgatt
    69301 tcaacgtcac cgatcagttc aggggaatgt atgtggtaaa agtggagtcc gccgaccggc
    69361 gatggctgca ggatgccgtc atggtatcgc tttccgacct gggcatgatt gtgaaggaag
    69421 gcacagataa tgtgctcgtc tttgtcaatt ccctgtttaa cgcttccccg gttaagggcg
    69481 ccagagtcga tttctacagt tcgaacaacc agaaaattta tagcgctacc acggacagcc
    69541 gcggaactgc tttgtttaaa aacattaaaa gccaggcgcc gggattcagg ctcagcttgg
    69601 ttactgccaa ctaccaggaa gatttcaact ttatcctgtt caacagtacc cgggtggaga
    69661 cttcccgttt cgatgccggc ggcaaacgca ccggcaatct caattacgac gtcttcctgt
    69721 atggcgaccg cgacctgtac cgcccgggcg atacggtata ctataacgcg atcgtcaggg
    69781 acctcgggtg gcaaaccgta cgcgacctgc cgcttaagat caggatattg gcgccaaatg
    69841 gtaaagaata ccaaagccag cggaagcagc taagcaacaa cggggccgcc gaaggttcgt
    69901 tttatattcc tgttgcggcc atgacgggaa cctattccat tgaagtattt tcttccaaca
    69961 atattctgtt ggcatccagg cgcgtcgctg ttgaagaatt tatgcccgac cggataaaag
    70021 tggccgtcag cacggcaaag aacgcttttg attccggcga gtccatccag gttgaccttc
    70081 aggctgataa cctttacggg acgccggctg ctgggcgcaa attcgaaacc gaactgcgcc
    70141 tttcgcgtaa aaacctgaag ccgaaagctt ttcctgatta caattttacc gtcgaaacga
    70201 agaacatgcc ttatctggca accattacca ataccgggac caccgatgcc tccggtaaag
    70261 caagccatct gataaaaccc ggcgtttacg agggaatcgg catccttgac gggactttgt
    70321 tcacaaccgt tttcgacgaa accggccgtc cggtcaaccg gctcaataaa atcgaagtac
    70381 tcacccaacc ggttttcctg ggaattaaaa acttcgacca atgggtcggc acccggaaac
    70441 caatgaattt caaagttata gccgtcaaca aagatgggaa ggcgcaggga aacgtccagg
    70501 cacaggtggt ggtatcgcat ctccgctatg aaaccgtcat cgaacgcgac ggtgaccggt
    70561 atcgctatgt atcgcaggaa aaagaatcgg ttgaatattc aaagacggtt gccgttccgt
    70621 caacaggcgc ggtaattcct ttcaccccgg cgcaatcggg caaatacgtc ctgaaagtaa
    70681 tgctgccggg cagccagaat tatgtctcat ccacatttta tgcctatggc tggaatgaca
    70741 ccgatttcac ttcgttcgag gttaaccgcg aaggtgaagt aaccatatcg gccgataagg
    70801 agtcctatca gccgggcaac aaggccaaac tgttgttcag cgcacctttc gacggcaaaa
    70861 tgctggtgac cttttcgcgc gacaacgtcc ttgaattcaa atatattgaa gtaaaaaaca
    70921 aagcggccag cctggt
//
LOCUS       DAOPYV010000002        67999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_265_length_67999_cov_10.330360, whole genome shotgun sequence.
ACCESSION   DAOPYV010000002 DAOPYV010000000
VERSION     DAOPYV010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 67999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 67999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67999
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_265_length_67999_cov_10.330360"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1654
                     /locus_tag="PKI35_00260"
     CDS             14..1654
                     /locus_tag="PKI35_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX42839.1"
                     /translation="MKDSLSLTFPGFFAQTAERFGDRPAISLIGENPLTYAELDDKIK
                     SLMAWLEALGVKQGDKMAILSISIPNWAVSYLAVTFMGAVAVPVLPDFHPDEITNILV
                     HSGAKGIFVSDGLRSKIENTGNDELIKIRIEDYSLFNGEAPAGLFTPGVIPQGHYAIS
                     PDDLAAIIYTSGTTGNPKGVMLTHRNICYNAIQCSTVHPINENDRFLSILPLSHTYEN
                     TIGLIYPLMRGASIHYLGKAPSPSLLLPALKIVRPTAMLSVPLIIEKIFRNKIQPEFR
                     KNIFLRIAVHIPFIRKKLNVVAGRKLMSTFGDELRFFGIGGSKLNKTVEKFLIEARFP
                     YAIGYGLTETSPLLAGTSPGHFRLQSTGPAIEGVELSIFKPDPKTGEGEIWARGANVM
                     KGYYKEPGLTEEVMTPDGWFRTGDLGVFDRDGYLFIKGRSKNMIVRAGGENIYPEEIE
                     SIINNFRHVIDCLVLDQKGKLVALVQFNTDEIAERNKYLKDEIKDYVEHKIEELRLEL
                     QLYVNHRVNKFSQIQVVIVQQEPFEKTATQKIKRYLYR"
     gene            1840..2304
                     /locus_tag="PKI35_00265"
     CDS             1840..2304
                     /locus_tag="PKI35_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNX42840.1"
                     /translation="MGRSQETFNKKEVRNKNQKKRKEKEQKRLARKNSDKSGNQEDMI
                     AYVDEFGMISSVPPDPDKKLEIKTEDIRISTPRKDSESDADNQRKGTVTFYDDAKGYG
                     FIKDSDTGESIFVHAKILQETVNAGDKVFYEAERSPKGYSAIWVKHIDEKGA"
     gene            complement(2724..2879)
                     /locus_tag="PKI35_00270"
     CDS             complement(2724..2879)
                     /locus_tag="PKI35_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42841.1"
                     /translation="MKEKKQVNATSPDLSKLQAVVVDHNTMIYIPADADPEEAKERYQ
                     ARYAKKK"
     gene            complement(3226..>3489)
                     /locus_tag="PKI35_00275"
     CDS             complement(3226..>3489)
                     /locus_tag="PKI35_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42842.1"
                     /translation="FGGSMIWFGSSAGVALSNMYPEAKSVVKYVKHGWHVTVAYIVGF
                     FVMLFTVGWHPHAPHRAKDKAQQEVVMPASMNSDTLIQQPGAN"
     assembly_gap    3490..3589
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3590..4733)
                     /locus_tag="PKI35_00280"
     CDS             complement(<3590..4733)
                     /locus_tag="PKI35_00280"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNX42843.1"
                     /translation="MKKKKTLGCLFLTLLFFLAPVMSFAATTAAMPLVFGIRLEFVIF
                     ALTLVGVAIFHHKTMYVALAGLAVLLILKYAFLPEFSLPEHIAGTPDKEGEWRTLLNL
                     LGLLFGFAILAKYFEESGIPEWLPKVLPDDWRGGVVLLFAIMVISSFLDNIAAAMIGG
                     AIAMVVFRGKVHIGFLAAIVAASNAGGAGSVVGDTTTTLMWIDGVNPLHVIHAFIASI
                     TSFLIFGIIGAIQQHRFQPIVKNLSKSVKIDWGKLIIVLMILVGAIITNWTLDFPAIG
                     VWVAILIGNFIRKAPWNEVPKSLQGTIFLLALVTCASLMPVDELPKASWQTAFSLGFV
                     SAVFDNIPLTKLCLEQGGYDWGVIAYAVGFGGSMIWFGSSAGVALSN"
     gene            complement(4836..4967)
                     /locus_tag="PKI35_00285"
     CDS             complement(4836..4967)
                     /locus_tag="PKI35_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42844.1"
                     /translation="MNEVTDNKSNEAPKTHKGFLPFLLVFGGIVIILILIKLLINMI"
     gene            complement(5117..5470)
                     /locus_tag="PKI35_00290"
     CDS             complement(5117..5470)
                     /locus_tag="PKI35_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42845.1"
                     /translation="MRSITTCCLIILLLPFLMEVRTGTELQQNLSKQANGGKIPFSLQ
                     VAGTDMFVLPGQDGTNGSRDNLERYKNQLCYWKIIKPLVSEAPAFHQSGNSVYRDSFL
                     SETFTLFIAHHNLRI"
     gene            complement(5650..6039)
                     /locus_tag="PKI35_00295"
     CDS             complement(5650..6039)
                     /locus_tag="PKI35_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNX42846.1"
                     /translation="MSKNRKIAAPITADHQIDDHFGHCEYYGVYSVTENNEISDVSII
                     PSPQGCGCKSNIAGVLADQGVTLMLAGGIGDGAVNVLNHWGIDVVRGCNGDARDAVKA
                     FLNGSLTDSGQNCLHHHQDGDGHQCNN"
     gene            complement(6071..6856)
                     /locus_tag="PKI35_00300"
     CDS             complement(6071..6856)
                     /locus_tag="PKI35_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNX42847.1"
                     /translation="MNVNEKKTLPGVKQIIVVASGKGGVGKSTVAANLSIALARNGFK
                     IALVDADIYGPSIPRMFGIEDVVPEVSAVGEKEIMYPIEKYGIKIISVGFFVKQNQGL
                     IWRGPMAANAILQLFENTQWGDIDFMIVDFPPGTGDIQLSTVKQLNVSGAIIVTTPQA
                     ISLNDARKGASLFTNSGINVPIVGVVENMSWFTPKSHPEEKYFIFGSDGGETLAKELN
                     TKFLGKIPLVAEVGEEAEKGFSVYHQADKEVIGAIENIASVLV"
     gene            complement(6864..7577)
                     /locus_tag="PKI35_00305"
     CDS             complement(6864..7577)
                     /locus_tag="PKI35_00305"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNX42848.1"
                     /translation="MENNLNQGNLPIPRIGEMSPSFKAVTTQGEINFPDDYKGSWVIL
                     FSHPADFTPVCTSEFMTFASREEQFNKANCKLIGLSVDGLYSHIAWLRTIKEKIEYKG
                     MKNVEVRFPLIEDITMEVAGKYGMIQPGESNTKAVRAVFFIDPKGIIRAIIYYPLSLG
                     RNFDELYRVLVALQTADAFSVATPADWLPGDDVIIPTAGSCGVAKERMEDKSSMHCYD
                     WFFCTKELAKEEIERKLFK"
     gene            complement(7642..8997)
                     /locus_tag="PKI35_00310"
     CDS             complement(7642..8997)
                     /locus_tag="PKI35_00310"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX42849.1"
                     /translation="MKIADVLIIGGSAAGMVAAVTGKSSWPDKSFILVKKQKEVLVPC
                     GIPYIFGTLDSSQQNIMPVDNMLEKAGIESIVGEVTFIDKKLNIVVLSTGESIQYDKL
                     VIATGSQPVKPKWLKGCDMENVFVIPKDKVYLDEMNYKLQGLKKIVVVGAGFIGVEFS
                     DELIKSGHEVCLVEKESSILASAYDTEIADRISSILQNRGVKVISGNGIREIKGSEKV
                     DGIVLENGDTIDAEAVILSMGYHPNSVLAVNAGIFVDESGFIAVDEYMRTHEKDIFAV
                     GDCAQKRDFFTREKVSTMLASTACAEARVAGMNLFGIHVVKTFSGTIGIYSTAIGENG
                     FGTAGLTERRAQDEGIAVVTSVFEGVDHHPGHMPGSHKQLVKLIVAKNSGVIIGGEVI
                     GGTSAGELINVIGLVIENRMTVNSLLISQIGTHPCLTASPAAYPLIKAAELIASKLRN
                     S"
     gene            complement(9015..10196)
                     /locus_tag="PKI35_00315"
     CDS             complement(9015..10196)
                     /locus_tag="PKI35_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX42850.1"
                     /translation="MNTKNKGFDTKLIHAGAFDNEFGSACVPIYQTSTFGFRNAQHGA
                     DCFSGKSDGYIYSRISNPTIRAFENNIAELENGFDGIATSSGMGAVSSVFMALLGAGS
                     HIVCSDAVYGPTRGILENDFSRFNVEASFVNTSETENILSALKSNTKVLYIETPANPT
                     MGISDIEACAKIAKEHNLILVVDNTFCSPYLQNPLKLGADVVLHSITKFINGHADIVG
                     GVIVTKGEDLYKKIRHSMVYMGCNMDPTQAFMVLRGVKTLSLRVERAQENAMKVAEFL
                     QAHPKVAWVNYPGLKSHPQYELARRQMKGPGSMMSFGLIGGYEAGKKLMDNVHLALLA
                     VSLGGVETLIQHPASMTHAGVSKDNRLAAGITDDLVRFSVGIEDADDIIMDLENGLNK
                     I"
     gene            complement(10253..10765)
                     /gene="cobO"
                     /locus_tag="PKI35_00320"
     CDS             complement(10253..10765)
                     /gene="cobO"
                     /locus_tag="PKI35_00320"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNX42851.1"
                     /translation="MKGYVHLYTGDGKGKTTAAFGLSIRAAGAGKKVFIAQFVKGMKY
                     SELKALERIPEIEVRQYGLKCFIISQPTEADFQAARRGLKEITKLIIGNKYDVVVLDE
                     ICIALYYNLIGLEEVLKLIEKKPLPMELVLTGRNAPEALVERAELVTEMKEIKHYYNE
                     GVEARRGIEF"
     gene            complement(10769..11716)
                     /locus_tag="PKI35_00325"
     CDS             complement(10769..11716)
                     /locus_tag="PKI35_00325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX42852.1"
                     /translation="MVRTLIFGPVPSRRLGKSLGINNIPYKHCSYSCLYCQVGKTIKK
                     QVSRCEFYDPDLLADGVRKYLDALSEEDMPDYVTIVANGEPSLDLNLGFLIKKMKQNG
                     LPVAVITNASLITSKEVREELGQADYVSVKVDTMNEKTWKTLNRPHSSLVLQHIKDSL
                     LIFKEDYSGKLVTETMLVKNVNDSESEIYETSAFISSIFPDIAFISIPTRPTAFNKAY
                     RPAEGNLVKAYAIFEQRGLHTEFLIGYEGNAFASSGNFTKDILSITAVHPMRREAVLE
                     LLQKSKSSERELNLLLSYGMIKEITYNNQVYYQRNFSKK"
     gene            complement(11872..12219)
                     /locus_tag="PKI35_00330"
     CDS             complement(11872..12219)
                     /locus_tag="PKI35_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNX42853.1"
                     /translation="MKIAFTATGETLDSQIDPRFGRTDFILVLDEETNNLEVINNLEA
                     NNEAHGAGTATSRKIYQFRPDVLITGNGPGDTAAKALKPLNMKIFTGAYDVTVRKAYE
                     LYKQGQLKEISML"
     gene            complement(12229..13104)
                     /locus_tag="PKI35_00335"
     CDS             complement(12229..13104)
                     /locus_tag="PKI35_00335"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX42854.1"
                     /translation="MNEIVILSGKGGTGKTSIAAALASLAGNEVVIADCDVDASNMHL
                     LLQPDFHFSEDFYSGHLARIHPEKCMKCGLCIMKCRFEAIILTGDDYMIDPVSCEGCG
                     YCQMICPLQAISMEERLSGKFYISRTRINNVLVHASLDIAAGNSGKLVAKVKSEARKI
                     AVSEHIPFIIVDGPPGIGCPVISSLAGATFVLIITEPTVSGIHDLERLIPLIRHFRAR
                     MACIINKSDINISKTKEIHDYLKQQNILHIADIPYDSLFMESMVKGLTITETGSPLSG
                     QLVNIWTTIKEFLKN"
     gene            complement(13101..13982)
                     /locus_tag="PKI35_00340"
     CDS             complement(13101..13982)
                     /locus_tag="PKI35_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX42855.1"
                     /translation="MAVSADIKRIAIASGKGGTGKTLLSTNLAACISRSYPTLLVDLD
                     VEEPDDFLFIGGVLQKSLVQYKFIPDWNETQCTFCDSCSDHCRFHAILRLGESIVVFN
                     ELCHSCFACSELCPSGAIRMKPQKIGETKEFVTGNLRFIHSCLEIGEEQAVPLIHKTL
                     QLVRNQTGNLPVQLYDCPPGASCPMVAATKDADFVILVAEPTPFGIHDFMLAASTVRM
                     LGKPFAAVINRSDMGDDSLEDYCNTGNIPVLAKIPFSNKISKLYSEGSLVFGNCDVMN
                     PALEKIADYIIQLKLNP"
     gene            complement(14207..14356)
                     /locus_tag="PKI35_00345"
     CDS             complement(14207..14356)
                     /locus_tag="PKI35_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42856.1"
                     /translation="MVSVEIQDMCIGYFGMKPGCFQKRISGEVKLVVNTSASLAEIMD
                     MAAKE"
     gene            complement(14540..14914)
                     /locus_tag="PKI35_00350"
     CDS             complement(14540..14914)
                     /locus_tag="PKI35_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNX42857.1"
                     /translation="MNKRIAIPMEDGVLCAHFGHCQTFAIVTIQDHQIANIRDVVPPE
                     HVPGLYPRWIAQFGVTDVIAGGMGQQAISLFNEQKINAFVGAPIKTAEEIVRDFISGK
                     LELTANYCDHDDDHHHDCAQGK"
     gene            complement(15412..15738)
                     /locus_tag="PKI35_00355"
     CDS             complement(15412..15738)
                     /locus_tag="PKI35_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNX42858.1"
                     /translation="MKVAIASTGNTPESNVDLKFGRCSCYVIYDTESRSVEYIPNPYK
                     TDEESAGTSAVQLMVSRNVARIIAGEFGIKIKGLLDSLKIQLIIVKDRGRTIREIIEL
                     LNNQTN"
     gene            complement(15865..16320)
                     /locus_tag="PKI35_00360"
     CDS             complement(15865..16320)
                     /locus_tag="PKI35_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNX42859.1"
                     /translation="MSPRFKRPRKLVNPPVIKGLKPYGPGVPEAVSGPVTLLYEEYEA
                     LRLCDYDRYNHIRSSVIMNISRPTFTRIYASARQKIAEALVEGRQIIIEGGKVYFDST
                     WFECDTCNACFNNPDWETPVTACPLCGSNEIHSFEDEIPETTAVTEADH"
     gene            16409..16774
                     /locus_tag="PKI35_00365"
     CDS             16409..16774
                     /locus_tag="PKI35_00365"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNX42860.1"
                     /translation="MDVPIVNQFHVVMSGDLNDRNCLFGGQVLKWMDETAYVSASIFA
                     KAEVVTVSAEKIRFLKPVFAGNLINLKAEVIKSNGLKIIIHTIALASADKALTWDTIA
                     EAEFCFVAVDKNNAPKRIR"
     gene            16778..17398
                     /locus_tag="PKI35_00370"
     CDS             16778..17398
                     /locus_tag="PKI35_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNX42861.1"
                     /translation="MKLSEELRKDVRFSEGMKACINCGTCTAICPAAHFYDYDPRLIA
                     ITVQSGDEKELEKLLKGDTIWYCGECLSCKTRCPRGNTPGYIVQALRNLSIKTGYFSF
                     SERGRQQVRVKRIVGENILKRGYCFYIDEINTTDHPEQGPIWDWYRKNAVSILEKTGG
                     NYKAEGPGTLRDIQPSDMEELHRIFAETGAIEQFNAIEDYCDGKKK"
     gene            17382..18452
                     /locus_tag="PKI35_00375"
     CDS             17382..18452
                     /locus_tag="PKI35_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNX42862.1"
                     /translation="MGKRNDRVLWPEYQKNIAGDHYYFARSCIRQNFFPGSEELFLRI
                     LKEVLGLDIFDDAHQTTCTGIAYHSGILPVETTMTVVARQFSLMNDAGYRNFVPSCVT
                     SFGIYTEMVETWQHFPDTLEKTKAQLKAATGRSFRLPENIAHTSDIIYKFRDEIAAKA
                     KYRLVNARTGKPLKVVEHIGCHYAKIFPAKGVGGAEFPRVLSGMIEAWGGEVIDYPER
                     RHCCGFGFRNYIIKENRGYSISNTKRKLDSMEPYHPDFIVANCPGCTFFLDRWQYVLA
                     EMEGKTYDTEGYGIPVLTYEEMAGLVLGYDPWEIGMQLHQVSVDSLLNKMGIGYKSGE
                     KYLGASKEFIGEPEKPSVLKVK"
     gene            18716..20848
                     /locus_tag="PKI35_00380"
     CDS             18716..20848
                     /locus_tag="PKI35_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX42863.1"
                     /translation="MQRKALLIVALGLAITPFSGFGQQISDTINLKGVEIVGSGIPNK
                     TSLSKVNLDRAAIPDVGVLLRSVPGVNGIKKGGSSIDPVIRGFRSSQLTVIADDGLKV
                     EGGCPNRMDPVTSHIETDDIESIDIIKGPYTLRYGPSLGGVIKINTFKPHPYETFQIH
                     AKSHTSVNSNPEGISQYLLIHGGGQKIYFGISGSYRNYGDYRDGGGNLISSSFRKYNF
                     SGDVGYKPHPDHEILLSVHRNFTRDMKFPALPMDEIRDNTTLLSLDYHFRVRNSILKQ
                     LDFKVYNSDVYHEMDNSFRPAYSQVVPPYNGLMQAVSKVNAANTGGRLEATLVSGKQE
                     LITGADVEITGKDGGRVMTMIMNMGGIETTTAKRTNLWKEARTINSGLFSEYTLSLRD
                     YKIRLSARLDYNHAGSEDTLILIANDKTYFNDTRSDYLNISANAGISKAFGKGNQISL
                     FVGRGMRSPDMTERFIKFLLVGYDNYDYLGNPQLKPEVNYQADLILDLNLGKAGKFNI
                     SGYGSLVDSYISGVILPSTAATPKTMGAVGVKQYMNTGKAYFLGFETGYASPVIKGFN
                     IDLSAAYTYGILASSTRNIFDENNKPVGVEDVKNDAIQEIPPFESGINLNYAFLKEKM
                     QVSLQSRFVLAQNHVSLSYYENKTPAFMLFNLTYNYNLNRMFMISAGVQNLFNSTYYE
                     HLNRRIVGSQDKLNEAGRSFYLNLTISL"
     gene            20845..21645
                     /locus_tag="PKI35_00385"
     CDS             20845..21645
                     /locus_tag="PKI35_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42864.1"
                     /translation="MKHLPPVILLLCLFFAACEPEIIKPEKAGGKIVIAFLHHVDSSP
                     LITDSLCYVNEAGNVYEVNELKYFISDVYLIRNGEQSVHIEDETAIHYTDIDIPATLS
                     WKVYDTVPEGTYDSISFIFGLNEVRNVSFAFVNPPEVNMFWPDILGGGYHYLMLNGKW
                     KNPEGNTTPFDFHLGIGQIYSGTTTSVDSITGYVQNYFKVTLPVAGLEIKKNTTRTIG
                     LVMNIESWFCTPHVWDFNVFGGYIMQNQEAMHRAVENGRDVFEIGSIR"
     gene            21656..21871
                     /locus_tag="PKI35_00390"
     CDS             21656..21871
                     /locus_tag="PKI35_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HNX42865.1"
                     /translation="MPVFRKVFNFYYEGFRNMTVGKTLWIIIFIKLFIFFIILKLFFF
                     HDFLGSKFKSDKDKSDYVIEQLLTKPK"
     gene            21874..23403
                     /locus_tag="PKI35_00395"
     CDS             21874..23403
                     /locus_tag="PKI35_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNX42866.1"
                     /translation="MDVSLVDWSRAQFALTAIYHWLFVPLTLGLSFIIAFMESIYVKT
                     GDEEWKKITKFWMTLFGVNFAIGVATGIILEFEFGTNWSNYSWFVGDIFGAPLAVEGI
                     MAFFMESTFIAVMFFGWNKVSKKFHLVSTWLVAAGANLSALWILVANAWMQYPTGMHF
                     NPETARNEMLNFWEVLFSPVAINKFLHTVTSGFVLSSVFVVGISAWYLLRKRNQMLAK
                     RSMLIASVFGFVMSLFLILTGDGSARLLARHQPLKFAAMENLYEGQSNAPLVAVGILK
                     NDNTEPKKKDFLMKLEIPGVLSFMAFQNFDAYVPGIRDLVHGNPEQGIISVNEKIAKG
                     KLAIEALKEYKEAGKTGDTAKADSASAVLNSNFKYFGYGYLEKPQDAIPPIGPAFYSF
                     RIMVALGMWFVLLFILTVFFVFFRKPEKIKWYLYLSVLSIPLAYLASETGWLVAEFGR
                     QPWVIQDLMPASAAVSRLTTGSVQTTFILFAVTFTLLLIADIKIMSKQIKIGPKDGGE
                     K"
     gene            23403..24542
                     /gene="cydB"
                     /locus_tag="PKI35_00400"
     CDS             23403..24542
                     /gene="cydB"
                     /locus_tag="PKI35_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNX42867.1"
                     /translation="MFENLSYPALQQYWWIIISLLGALLVFLLFVQGGQTLIYRLGKT
                     ETERALLINVLGRKWEFTFTTLVTFGGAFFASFPLFYSTSFGGAYWVWMLILFAFVLQ
                     AVSYEYRKKPDNFLGHRTYEAFLFINGAAGTILLGTAVSTFFTGSQFSVNENNFSQWE
                     TATHGLEAAINIQNLSLGLAVFCLSRVLALLYFMKNVNHDEIIARAKKQLLVNAIPFL
                     VFFLLFIMLLMLKKGFAVDPLTGLVFMEPFKYLHNMIQMPVAGILFLTGVVLVLTGIF
                     LPVFSFQSFGRQGILFAGTGSILTVFALFLIAGFNHTAFYPSVFDLQSSLTIYNSSSS
                     RYTLTAMSYVSLLVPFVLAYIIYTWRAINRKKIDISELENESHTY"
     gene            24703..25020
                     /locus_tag="PKI35_00405"
     CDS             24703..25020
                     /locus_tag="PKI35_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42868.1"
                     /translation="MMMVRKLKAIVALLLITALFWPSVIKLDHHHFYIPVPGTGEQLT
                     VVHEKCPACSFEYSIFISDSGVKGPERAEVICERFCFILPLHILFSPRYSFFLRAPPE
                     KMI"
     gene            25079..27322
                     /locus_tag="PKI35_00410"
     CDS             25079..27322
                     /locus_tag="PKI35_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX42869.1"
                     /translation="MKSIFIICLLVLLYNTGMAQNTLYGKVTDQDNTPLAGANVYLPE
                     LNKGTVTDASGNYILKNMSNGKIKIQFSYLGYTSRIETIVLNHQDTRVDVSMHKTMIE
                     TEGIVVTGGYSSTQHENAVKIDILKLGLENLKCTPNFMESLTRVPGVNMISKGSGITK
                     PVIRGLSMNDILVLDNGVRFENYQYSSHHPLGIDETGIESAEIIKGPASLLYGSDAIG
                     GVINFIREKPASVNSIEGDYNVNFNSNTLGIANNLGIKGTSGRFFGGIRAGMKYNADF
                     LQGGGQFVPNSRFHEYSIKTNAGYTGKAGTIRLFYDFIRQNLGLVEEEALEAIKERGY
                     KCEIFYQQLNTHLLSSQSKLFLGRTKLDLNASFQNTGLLHVGEEDEYEIHMKLATLSY
                     EAKLYLPSDESSEYIVGYQGINQFNTNLHNRETILLPDAKTTNQSVFGLAQRTFFRKM
                     KTQAGVRYDLKWISTNSVGLPGNSSYREALDRNYGSFSGSLGATYNFSEKFLLRGNIA
                     AAYRTPNLAELTSNGAHEARYEIGDKNLGPEKSLEFDLSLHYHIENLTIDLAGYRNNI
                     RDFIFISPTGEITGNGLPVYRYLQQNSYLYGGEAGLHYHPEHLKWLHIETTFSSVTGK
                     HSDGSYLPFIPANIINLNLMIRKNKLGFMKNASVSVDFSKTFDQSNPAPDESATDGYF
                     LTDISAGGEFSLQKHPVMVRLGATNLFDVRYTDHLSTLKEVNLFNPGRNISVFLRIPF
                     SSQLYNK"
     gene            27576..27992
                     /locus_tag="PKI35_00415"
     CDS             27576..27992
                     /locus_tag="PKI35_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42870.1"
                     /translation="MKNTVRLFAAMALTAFFITGLQAQTTSADQAKTRQKSTAINRPA
                     FVDKNNDGVCDNYSSGRYQGRGANFVDKNNDGVCDNYGSGRYQGRGANFIDSNKDGIC
                     DNRQGATGKWNSGCSMGNGNRHRHGNGHCCGSLKQN"
     gene            28108..28746
                     /locus_tag="PKI35_00420"
     CDS             28108..28746
                     /locus_tag="PKI35_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNX42871.1"
                     /translation="MVTGDLYFNLLLRSELMTDQELITGIRNRDRQVIRHLVGTYHKQ
                     VIKTAYNFTDNMDDAEDIAQDVCIEILESVKHFRGDSSLSTWIYRLTVNKSLNFIRKN
                     KRKQLVRQFETFFRIAANGAWNGIPEPYSTEDPVQKNESRILLDDAVSRLPENQKTAF
                     ILNKYEEMSYKQVAEVMNLSLSSVESLLQRAKQNLQKSLIAQFSEYSKTGTS"
     gene            28743..29186
                     /locus_tag="PKI35_00425"
     CDS             28743..29186
                     /locus_tag="PKI35_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42872.1"
                     /translation="MTDCRLLHENLYGYAAKELPDTLMDQLDQHIAVCLDCKQLLEQF
                     NDVLNAIGEHVGAEPLPYAGSRLLHRLEAALEMPEKSSFVFSKSILQPALISLGMLLA
                     LAIGFLIGTEGAGRYAAYNENTIQEVRSDLNVPDFMTDELLEFTE"
     gene            29198..29752
                     /locus_tag="PKI35_00430"
     CDS             29198..29752
                     /locus_tag="PKI35_00430"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HNX42873.1"
                     /translation="MDFLKKNRIVFWVLIFLVFVNIAALATHFISLQKHAAEPVSSPE
                     GRKGVAIGRELSLSPEQLNKVNKINTAYRFSSEPIITALRDKKAALLEELSKDTSDSL
                     RIAVLAEEVSREQNKLQQANIRQFLDLKKVCTPEQTQKLSGIYAGFYGCAGAGRGQGC
                     GNGNGKGYRHRHGWKQQDTISNGE"
     gene            29782..30048
                     /locus_tag="PKI35_00435"
     CDS             29782..30048
                     /locus_tag="PKI35_00435"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNX42874.1"
                     /translation="MNTKIQKNIAISDSGFLFNPTTGESFSVNPIGLDIINLMREGRS
                     ESEICTTLLESYETESVMIERDLHDFIHMLRQHQLLENNDGEDH"
     gene            30029..31096
                     /locus_tag="PKI35_00440"
     CDS             30029..31096
                     /locus_tag="PKI35_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNX42875.1"
                     /translation="MTERIINIGVTGLNAIDSPGPGVPVIRGLREATSFKTRIIGLAY
                     EPLEPGIYLDALVDKTYLIPYPSAGTDMLLARLEHIQSIEKLDVIIPNFDAELYSFIK
                     LEPALKKIGISMFLPTMEQFEERHKVNLNTFGEKHNMDVPESKAVINLVDVVKTAKEM
                     GFPVMVKGKYYDAYVAATTDQARNYFEKVSAKWGLPVILQKFVKGTELNMTGLGDGNG
                     NTIAAVGMRKQFITDKGKAWAGITIHDYRLMELTRHFITTTKWRGGFELEIMKSSDNT
                     YYLMEINPRIPAWIYLAIGVGQNIPEALVNLALGRKVEPYETYEAGKLFIRYSYDMIV
                     SRERFEELSVNGELENDIEKP"
     gene            31178..32476
                     /locus_tag="PKI35_00445"
     CDS             31178..32476
                     /locus_tag="PKI35_00445"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNX42876.1"
                     /translation="MPGKFGMRTDYPPTAQIDGIPVKSLLEKYGSPLFVLSEDTIRTT
                     FKRAQRAFTNRYPKVQFAWSYKTNYLDAVCRIFHQEGSWAEVVSGFEYDKALHNGVDG
                     KKIIFNGPGKSGSDLKKAIENDSLIHIDHLDELYLLISIAKPDKKPRVAIRVNMDTGI
                     YPQWDRFGFNYENGQAWDAINKIVSSGKLELTGLHTHIGTFILTPNAYAVAATKLADL
                     ASEISHKFNLKIKYIDMGGGFTSRNTLKGSYLPGPDVNPSFDDYAEAISSALLNSRLN
                     VDALPLLILETGRALIDEAGYLAGSVIANKRLASGKKSVILDMGVNLLFTAFWYDHKI
                     TPAQPFSLYREDTVLYGPLCMNIDIIGDNISFPQLSVGDGVVIHSVGAYNMTQWMQFI
                     TLRPGIVLIDTKGNTHVIRDKESIGTIIENEKVPEHLKNF"
     gene            32515..34743
                     /locus_tag="PKI35_00450"
     CDS             32515..34743
                     /locus_tag="PKI35_00450"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HNX42877.1"
                     /translation="MRYLQTDKLKESLTDHFAGFLNSYAMVFFSKDRLFAGILILVTF
                     FDPVAGLSGLVSVLSANLVSGILGLNRFNVRSGFYGFNALLVGLGIGLYYQLTLELVI
                     LLVFSSLLTLFITVLLEGVIGKYKLPYLTIPFLLTFWLVTLAARYYAELHISERGVYQ
                     INELYSLGGKRMLQLNDWFSTLPLAPSIVTYFKSLSAILFQYHLFTGMLVAAGLLWYS
                     RIAFLLSLTGFYSAYLFYMVIGANFNELNYSFIGFNYILTAIAIGGFYIVSSRTSFLW
                     VILLTPLISILVTSTSVILGTFQLSTYSLPFNLTVLLFLYTLKFRDNRQTGLHLVSLQ
                     EYSPEKNLYTQRNNETRFPESSYYPFSLPVMGEWKVTQGHSGEFTHREGWRHAWDLEI
                     ADDKGSVFSGKGLMPGDYYCYEKPVVASANGWVEEILDNIPDNEIGQVNLQQNWGNTI
                     IIRHSDTLYTKICHLKKGSFKVKKGDSVKKGEAVALCGNSGRSPVPHVHFQVQSTPFI
                     GSLTIDYPLSQYLLNTEKGYEIKSFSRPVTGDIVSNLNPNSCLGKAFDFVPGQTIEFS
                     VSADGCVPSVIRWDVMADIYNRTYIQCRQSGSKAYFFSNSDIFYFTWFEGNKKSLLFA
                     FYLGAYKIAKGYYRNLTIRDIYPVKVCGNGLFKMLQDFVAPFFLFLHNEYEMCYCSME
                     DELEQRSIKMKSSARKQICKRSSSMAEFEFLIDNDRVEKFVITEGKHITTAIRSINPQ
                     NF"
     gene            34794..36140
                     /locus_tag="PKI35_00455"
     CDS             34794..36140
                     /locus_tag="PKI35_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX42878.1"
                     /translation="MMTAILILLSVRGYSQEKDFRYYDSLSYGLYTLKDWKSLIAVAE
                     EAESKGYDYYYLNMRLGIAHYEMKDYRSSIPYFEKSLEYSTDEPAAVEYLYFAYLESG
                     RSGDASMLAAKYREKLASVPGTGSKILNFIYTEGGYTPDAASRLTPSALIGNDSIYGE
                     EDAFKTQSYFHLGIQLQPLPFLKVYASGSMLGIGKQKHFAFANYNASGNFLSVIDTVF
                     PYSYNQKEFYIAASLSPYRGFTITPALHVMQGDPSPVNCIYSDYTYSFSEDAYSYSHY
                     VFSLLLTKEMRKFTLGVSGSVSKLTPKGRQYQASASLTWYPKGNLDIYTTSIISVLRE
                     NKKTRLIYEQTVGLKMAPKLWLEGSATVGDLAYFNEKNAFVVYNLPEHIVFRGGASLI
                     YMLNRHLDVSFMYRLYSRENDYLYYSYDPDRGRLISQTRKNAYVNQSFFGGLKWKF"
     gene            36128..36760
                     /locus_tag="PKI35_00460"
     CDS             36128..36760
                     /locus_tag="PKI35_00460"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX42879.1"
                     /translation="MEILKKPVTWIVLLFNLNIGIIHLYAQDFQKLQNAFIASYSYEN
                     NKNYAKAIDALKQVYDGQSYEINVRLGWLYYYAANYPESIKFYSKAIQLKPYSVEARF
                     GYALPASAMGNWNDVLKKYFEILEIDKNNYTANYRIGLIYYNRKDYATAYRYFEKLAN
                     MFPYEYDVTHMFAWTNYRLGKLREARVLFQKTLLIKPGDSSAAEGLKLIK"
     gene            36923..37651
                     /locus_tag="PKI35_00465"
     CDS             36923..37651
                     /locus_tag="PKI35_00465"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNX42880.1"
                     /translation="MKKISQIFLLLLVAAGLPFSSVANPGNDGAISGNGKVTTQNRTI
                     GSFHALKVSGGIDVDLSQGNDIKLQVEADENLIENIQTVVKDGVLNIYPEESVRKAKV
                     MKIHLTFRDIDAINASGGCDITSTQKLDFTTLQTDLSGGCDIKLELKVNNLVSKHNGG
                     CDATFNGEAENCTLKVSGGCDVKASDFHIKNCSVDASGGSDVWVYATGILDLKASGAS
                     DVTYAGSPSKVTKSASGASDIHCK"
     gene            complement(37846..39858)
                     /locus_tag="PKI35_00470"
     CDS             complement(37846..39858)
                     /locus_tag="PKI35_00470"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX42881.1"
                     /translation="MKKLLLLIIWAIAFSVASIGQNNSPVAVNDTLYGFIGQTYTLNI
                     LQNDFDPDGDPIYVYYLRPPLTQINDTTFELTMSEYCFGTMYESSCNVEYLIKDASGL
                     TAKGYVVFILKYPPRYEKIDINNINALISPFGNHFWDGDTSRFEVPKGSGKTAIFNHN
                     LWIGGWDQNGRLCLAGEQFRLDGNDYSPGPVSMVKDTSYVKRWNRVWKISNSEILYHI
                     RNWNSTGYTPIEAIASWPAHGDVSLGQRAKIAPFYDADHDSVYNPMAGDYPLIRGDQA
                     VFFIMNDSMQEHTETLGYKMGVEIHGMAYEFDKPNDSVLKNTLFIHLDIINSSLSDYD
                     SAFIGLYTNFDLGNSQDDFAGTDVTHGVVYAYNGTAVDGNGEPQAYGEHPPAIGLRIL
                     GGPFMANDGIDNPEGECNYDINGLNFGDGIIDNERFGLTGSTCVINDPMNYDIYPNAS
                     TEYYNCMQNRWSDGTFLNYGGSGNLSTGAVGPECRFIYPGDSDTLCNWGTNGALPNGG
                     YNQNGYYWTEVTSGNTPLDTRMLAISGPFTFEAGQQQPLDYCVTFARDYDGDNLSSVR
                     LLLDNMASITPSVNQLIKLPGIFCSIPDKDRTGKMQVFPNPAHEKVRVVVEGDAGSKY
                     GLYDFSGKLLKEGYLQEGANEIDIRNLKPGVYILKSLGSRAKIIKI"
     gene            complement(40319..44746)
                     /locus_tag="PKI35_00475"
     CDS             complement(40319..44746)
                     /locus_tag="PKI35_00475"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNX42882.1"
                     /translation="MGILLVVFLLPLPAYILLRSGPVQTYITRQIAGNLSKELKTEVY
                     VGGVDISWFLNVVVKDVRINDRHHHTLIAADKIKLGYGKISKSERVINLSRIMLDKAD
                     INLVQYRGDSVLNLQFIIDYFSKPGPPDTTAKPWIIRCKTLKISGSHFNYCRQSKAGK
                     AEGMDYDNIDISSLNLKMRNVSIKGDTISAMIKSLSAIERSGFCIRNFSGLATVSSRD
                     LSVQQLHVVTDNSRLDLDLEFDYPAYHAFNDFLDSVYINSRLRPCELDMRDIVFFAPE
                     IKGMNQVFGIAGNLKGTVTSFKGKGMNITYGKNTVYRGDVTLNGLPDWTETFAHFKID
                     NLTTSADDIAAFGLPGENQRIKLPQEFYRFGMITIKGRFTGFYNDFVSNGAFWSDAGK
                     ISTDILLTNNKKKHIIEYGGTVSAENFDLGKILKTAEVGTLNLSANIKGKGLSLKTAD
                     LDFNGLVQNLVAKGNTLNSIRIDGSFRQEAFSGKVKLDDELARLNFDGVIDLSDSLPS
                     FDFRADMTDALLTRLNLIDRDTSTSLTTTMNLRFTGNSLDNLIGVLNFDNTVFYESGH
                     IVGMDRLQFQTSLLEGNNKKMTLVSDFADATFTGQYTFKDLRDYIMLIFTDYLPSLSK
                     GMSIIPQAHQGRFDYTIKLKNTKPVTGLFLPSLEFDTHTVLSGSFDPHGNLININGYS
                     PLIRISGLSLRDFTLNGVSQANKFTMSLLSGSIDASPGNVPDTSGFHIEKVQLTANAV
                     NDSVNWQFAWNDFDAGDRNKGALKGFVSFNEFPRLMATVDGARMVINDSLWVLNSDNL
                     LVIDSNNYSFQNLQLHCNRQKLSINGAISGDPLDLLTLSFENFDLSASDVLTKKLGID
                     FDGLVNGDVSVSELYNLPKAVSELTVKGFGFNHEQLGDFELLSSWDDVNKALSVDGRI
                     AYHGTAGLHYPLLVNGSVFPQRKHDNFDIDITADNLKVRLFEPFFTGLFSRMKGYGSG
                     KMKLIGDFSDPVLAGSVHLVRTQLLVNYIKTSYSFAGDLNFGKDKMWFRDIVINDSLG
                     NSGLATGNIYHNAFSDWRLDINLKADKMAALNTPYNPAEYFYGKAIATGDISITGPVN
                     DLSMKIKARSDKGTDIHVPINYSVNISDNDYIRYVNTKTRDSATREQPPREFSNLDLQ
                     LQIQATPDAALEIILPYQMGNIKVRGDGNVNMGIDSKGNYTMHGQYKMDEGNFLFSLQ
                     NIFSRSFQIERGGSITFNGSPDDADINLRAVYKVKPDLSGLISASAESTSGKRVPVNC
                     IISLSNSLYNPDIRFSIEMPDADPETQRIIYSVIDTSNQVAMNQQMISLLVLNSFSLN
                     QSTSTLSSNIGSSSFDMISNQISNMLSQISKDFDIGVNYRPGDQMTAQQLELALSTQL
                     FDDRVTIDGSMGMNQGATTATATSQSSNKWVGDVNVEVKITDDGRFRVKAYNRANNSL
                     DLNASRAPYTQGVGIIFRKEFDAVSEIFKRRSKQPGISY"
     gene            44838..45854
                     /gene="tsaD"
                     /locus_tag="PKI35_00480"
     CDS             44838..45854
                     /gene="tsaD"
                     /locus_tag="PKI35_00480"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNX42883.1"
                     /translation="MPVYILAIESSCDDTSAAVFADTTMLSNVVANQQVHKDYGGVVP
                     ELASRAHQQNIIPVINGALVSAKIDKKMLHAVAFTRGPGLLGSLLVGTSFAKAFALGL
                     DIPLIDVNHLQAHVLAHFIQETGKDRKTPSFPFLCLTVSGGHTQLVIVHDHFNMETVG
                     QTIDDAAGEAFDKAAKILGLPYPGGPFIDRNAAMGQPVFRFAKPRIPGLNYSFSGLKT
                     SFLYYLRDKLKTEPAFIQANMNDLCASMQQAIIDILMDKLIKAAKQYKIREIAIAGGV
                     SANSGLRKAILALNQSHGYNVYIPEFQYTTDNAAMIAVAGYFKYLKGDFADQSLAPYS
                     RGGI"
     gene            complement(45868..46149)
                     /locus_tag="PKI35_00485"
     CDS             complement(45868..46149)
                     /locus_tag="PKI35_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNX42884.1"
                     /translation="MFKVNEYFDGNVKSVAFNTSECPATIGVMAAGEYEFGTATIEYM
                     TVTSGILHVLLPGETTWKSYEPFSTFIVAKGVKFRVKAEGDVSYLCLYK"
     gene            complement(46490..47362)
                     /locus_tag="PKI35_00490"
     CDS             complement(46490..47362)
                     /locus_tag="PKI35_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42885.1"
                     /translation="MVLRFKPHTVLSCRILSGIAVAVILSSVFSSCTVEHRLGRKYIR
                     KEHNETLLVVPSDFIYKTNLTYQEPDPVNLISEEARDSAGFFRSKFLQHVSDSLFLEK
                     FYNAFISGMTSAGYRVMLCDDSIAFRAEPGPKWIISFAQLQMEEETKKYYYDTYDVDE
                     NYYYKEYELSTFGLNTWTEVEYQNAKPDQKRLLYLSGFIKEDFDGGFTYDSFDEKMYF
                     VEHSESRITDRDIYAMAAASGRKHAELMIDYLMNDYIRRNMPPGMTRKNILHFDRTFK
                     TLSTEVNERFEVLK"
     gene            complement(47398..48615)
                     /locus_tag="PKI35_00495"
     CDS             complement(47398..48615)
                     /locus_tag="PKI35_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNX42886.1"
                     /translation="MADNKVLKISDDVSWVGVLDRELVTFDVVMHTPYGTTYNAYFIN
                     ADRKTIVETVKVKYFDTFVEKLRTLCDPAELEYIIVNHTEPDHSGSLGKLLEIAPKAK
                     VVGSGNAIRYLRDLLGFDFTHIQVKDQDVIDLGNKKLRIIGAPNLHWPDSVYSYLEED
                     KLLFTCDSFGAHYCDEAMFDDLAGNWDDSFKYYFDVILKPFSRFMLKAIEKIRPLEIN
                     AILPGHGPILRSHWKKYVDMSEKLSRETLGQGTKPMVFIAYVSAYQNSATIGAKIAEG
                     AKQVAGVDVELHDIETMGLDKIDAYLSECTGLIVGSPTINQNILMPVYQLFAAVNPIR
                     DKGKLAGAFGSYGWSGESMKMIESNLTNLKLKFFGEGVFVKFTPHEEDYRKAVDYGRK
                     FAEQMLAGLSVTD"
     gene            complement(48765..51518)
                     /locus_tag="PKI35_00500"
     CDS             complement(48765..51518)
                     /locus_tag="PKI35_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HNX42887.1"
                     /translation="MEEKEDIFEEEDNESPVDQDEPAVETPEDDEAVSGELYRTMQLS
                     GMYQNWFLDYASYVILERAVPEVLDGLKPVQRRILHAMYELDDGRYNKVANIIGTTMR
                     YHPHGDASIGDALVQLGQKDLLIDMQGNWGNILTGDSAAAPRYIEARLSKFALEVAFN
                     PKTTQWKASYDGRNKEPVSLPVKFPLLLAQGVEGIAVGLASKLLPHNFNELIDASVAI
                     LKGEEFVIVPDFPTGGLADFSKYNDGLRGGKVRIRARINQLDKKTLVITEIPWGTTTG
                     DLIDSIIAANDKGKIKIKKIDDNTARNVEILIQLQSGVSPDQTIDALYAFSDCERSES
                     PNSCVIMNGKPNFCGVSEILRISTQQTVNLLKAELQIHLDELLEKVFFNTLLKIFIRE
                     GMYKNPKYENATSAEIVFKVLDTLFEPYYQEFYRPIEEEDYKRLIEKPMSSITKFDLK
                     KWDDTLKALCEEMEKVKYNLAHLIEYAIAYYMEIKRKYGANRERKTEIRNFEAIEAVA
                     VAAATQKLYVNREEGFAGTGLKKDEYVCDCSDLDDIIVFRQDGTFIVTRVAEKLFVGK
                     DVIHIDVFKKNDDRTVYNLAYQDGRMGAYYVKRFSVTGVVRDKEYNLTKGTKGSSVLY
                     FTANPNGESEVIRVSLKPKPRLKKLNFDFDFASLAIKGRASQGNILSRNPIRKIVQAE
                     KGLSTLSARDIWYDDTVMRLNTDQRGKYLGAFKEDDKILTIMKSGCYRLMNFDLSNHF
                     EEDMVVIEKLNPKKPVTCIYFNPEKGHYFVKRFLVEMTDKKVPFIPDEDGVTLHLIST
                     DYRPVLQVDYNNEGVKKPLENESLEVDSFVEVMGMKAKGKRLSSHPVADSYWLDPLPF
                     EESVVAETEETEETEDNEEELAGDESGTEEENTTETKPWPEQENENNRDDDDGPGIQM
                     SLF"
     gene            complement(51576..53636)
                     /locus_tag="PKI35_00505"
     CDS             complement(51576..53636)
                     /locus_tag="PKI35_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HNX42888.1"
                     /translation="MQFVNPLFLLGLLAVSIPVIIHLFNFRRFRKVYFTNVRFLRELK
                     QETQKKSRLRHLVVLALRMLAIVSLVMAFAQPYIPFSESRKPRASGNVVSIFVDNSFS
                     MEAMGPDGTLLEEARRKAREIVSAYQPSDRFQLLTNDFEGKHQRLVTREEFLLMLNEV
                     KPSPSVRTMKEIVSRQDDILKTEGKTEKTAFIISDFQKSVFKTFEDIPASGFQRYLLP
                     LKANTRENVYIDSCWFELPLQQTGQTSTLNVRVRNKSDKSLEKIPVKLSINGTQKAVA
                     TISLSEGGAGEIKIPFTIYQPGFQQAVVEVTDYPVTYDDKFFLSFEVTSSIKVLTING
                     GAENRYLNALFGQDSTVRMQNVNEKSLDYSALQNFNLLILNELPEISSGLNQELRRYV
                     DNGGTLAVFPAKSADIVSYNNLLAQAGCPVYMAVDTSNSKVITLNETDPVFRNVFDQE
                     PGRLPQNLELPLISQYYPIASKAVTLTVPVMKMLNGRDFLVETNSGKGKMYQFAAPLD
                     AGFSNFQQQALFVPVLYNIALMSRPPAFLYSIIGKDEAVWLNVPSPASDKVFRLKSES
                     GDFEIIPEIHRTGNGINLYARDQVKNAGNYFLMNGPAAIASLSFNYNRDESDLQCLTR
                     DEISAMISKLKDPSVSILETKNKPVDQLLSEINNGISLWKWFVILALMCLTAEAILLR
                     IAKK"
     gene            complement(53833..54039)
                     /locus_tag="PKI35_00510"
     CDS             complement(53833..54039)
                     /locus_tag="PKI35_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42889.1"
                     /translation="MIDYSEEFNELLIMQDCFVQNSGMFREMRGQNCKTAETITTAGE
                     NPDQDEMEFAFSRAWRDRANDDDL"
     gene            complement(54267..55319)
                     /locus_tag="PKI35_00515"
     CDS             complement(54267..55319)
                     /locus_tag="PKI35_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX42890.1"
                     /translation="MKSSNPGILALYLSASLTLLSGFAYWLSALAGGTKIPWVSLVIS
                     GAILLALQFFLIRYFLQSFIYAKIKLIYKTIHSLKLSKEEKRDLFKVTRGNVLDKVNE
                     EVADWASAHRKEMDALMQQETFRREFLANVSHELKTPLFNIQGFTLTLLEGGIHDPEI
                     NRDYLQKVEKNTERMITIVSDLEIISQLESGEAKPEMSRFDIVALTREVFEFLEAKAQ
                     ARQINLILANENIPPQYVSADKDKIRQVLVNLIDNSIKYGREQGRTKVSFYNMDENLL
                     IEVSDNGIGIEDVHLPHLFERFYRVDKHRSRVGGGSGLGLAIVKHIIEAHDQTINIRS
                     APGVGSTFSFTLKISR"
     gene            complement(55319..56002)
                     /locus_tag="PKI35_00520"
     CDS             complement(55319..56002)
                     /locus_tag="PKI35_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX42891.1"
                     /translation="MENNSFIILLVDDEEDILDFVGYNLRKEGYTVYTATNGRDAIDL
                     AVKVTPHLVLLDVMMPEMDGVETCREIKRIKSLDDAIIVFFTARSEDFTQILSLDAGG
                     DDYITKPIKPALLVSKVNSLLRRHKVVRETSPVFEAGDLLIDKEKYLVIREGKEIQLA
                     RKEFELLCLLASRPERVFTRDEILQKVWDDDVVVGERTIDVHIRKIREKTGSDHIYTL
                     KGVGYKFVR"
     gene            complement(56040..56717)
                     /gene="phoU"
                     /locus_tag="PKI35_00525"
     CDS             complement(56040..56717)
                     /gene="phoU"
                     /locus_tag="PKI35_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNX42892.1"
                     /translation="MSQLETAIQFLKNDLLQMWSLVISQMEKSRIAILEANKSLAHEV
                     RLNEKKVDAFDLKIDMDCENILMLKNPVAIDMRFVISVLKINYNLERIGDYTNTFAKI
                     AEGISFPWEEEVFRKTHIPEMFYLVQSMMQEAMEAFANQDKSLVNGLFQSDKKLDSYN
                     TEAVEIIGNLIREKPEQCNDYLNAYTVIGKLERSGDHVLNIGEELIFLYDAIVIKHIK
                     LAKGRKP"
     gene            complement(56797..57549)
                     /gene="pstB"
                     /locus_tag="PKI35_00530"
     CDS             complement(56797..57549)
                     /gene="pstB"
                     /locus_tag="PKI35_00530"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNX42893.1"
                     /translation="MIQAEAKNVNFYYGDFHALKDISMTFEPNTVTALIGPSGCGKST
                     FLRLFNRMNELIGNTRLEGTITVDGRNIYEKDVRVDEIRKKVGMVFQKPNPFPKSIYE
                     NVAYGLRVNGVENRKTIDEIVEKSLIQAALWEEVKDNLKKPAMALSGGQQQRLCIARA
                     LAVEPHLLLMDEPASALDPISTAKVEDLIFELKKQYTIIIVTHNLQQAGRISDYTGFF
                     YLGKLIEHDKTPKMFLNPADERTQSYITGRFG"
     gene            complement(57584..58456)
                     /gene="pstA"
                     /locus_tag="PKI35_00535"
     CDS             complement(57584..58456)
                     /gene="pstA"
                     /locus_tag="PKI35_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNX42894.1"
                     /translation="MNRFNKINRKQLSQRIAFSVFTLISLVVVGILFLILGFIVVRGA
                     SAISWEFLTGMPEEGMTKGGIFPAIIGTGCLILGSMVFAFPVGVLSGIYMNEYLAENW
                     FRRFIDFMTNNLSGVPSIVFGLFGMALFVNYLGFGDSIIAGSMTLGLLTLPVIIRTTE
                     EALKSVDDSLRTGSLALGATKLQTIRRVVLPVAFPNIITGLILSIGRVSGETAPILFT
                     VAAYFLPHLPQSIYDQVMALPYHLYVLATSGTNMEETRPMAYGTALVLIIIVLLVNLL
                     ANFLRRALGKRVKM"
     gene            complement(58476..59672)
                     /gene="pstC"
                     /locus_tag="PKI35_00540"
     CDS             complement(58476..59672)
                     /gene="pstC"
                     /locus_tag="PKI35_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNX42895.1"
                     /translation="MKQYRRTLEKLMEGILFSSSTITSLTVILIVVFLFREGLGIFTR
                     SPLDKNYVIAVSKANPVKDLSSDQLRAIFNQDITNWSRVGGKNDSIILFTTNDITNYY
                     SEEELGASFEYLPGKLDEFISTHPGALMHINNKDIPKDFPGRIIHVRNISVGDFLFGK
                     QWYPTSEPIPGFGIWPMIIGTLMVTFGALLIALPLGLATALFLAEVADIRVRNVVKPL
                     VELLAGIPSVVFGFFGLIVIVPFIQRVFHLDVGETVLAGSVVLGIMALPTIVTIAEDA
                     LRTTPRSLKEASLALGATRWQTLRRVTIPYAGSGISAAVVLGVGRAIGETMAVLMVTG
                     NASTVPHSFLVPARTMPATIAAELGEAPYGGAHFKALFALGIVLFLFTIAVNMTVAYI
                     KKGERK"
     gene            complement(59736..60557)
                     /locus_tag="PKI35_00545"
     CDS             complement(59736..60557)
                     /locus_tag="PKI35_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNX42896.1"
                     /translation="MKKMILMALAVLSIPVFAQKVSFKIKGSDTVLPLTQKEAEVYMK
                     KNPGSSIMVTGGGSGVGISALLSGTTDIAQASRSLKLDEKMKLNDAGKAFKETVIAYD
                     ALAVIVNPSNKVSKLTREQLEGIFTGKIKNWKEVGGDDMKIVTYSRETSSGTYEFFKE
                     HVLNKRNFSQSALLMPATGAIVQSVSQTKGAIGYVGLAYLGKNVKAIQVSFDKGKSYC
                     APSVEAAKSWKYPITRPLYYYYLKTIEKRVSPFVTFVLSPEGQKIVLNEGYVPIR"
     gene            complement(60639..61979)
                     /locus_tag="PKI35_00550"
     CDS             complement(60639..61979)
                     /locus_tag="PKI35_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42897.1"
                     /translation="MKFILRKNLTAGLVAVFLYLFPTAGDAQENDAAIAADTLNAKIE
                     NIKTDMGLLNRLRITGYIQAQWQKADTAGISSFAGGNFPKYSDNRFGIRRGRVKFSYE
                     NELSAFVIQLDATEKGVGVKDAYITVREPWAEFASLTAGIFDRPFGYEISYSSGTRET
                     PERSRLFQSIFPGERDLGAKLTLQPKKGTRFDFIKLEAGLFAGNGINAESDTRKDFIG
                     RLGMSKANKAENFKYGFGISYYNGSVLQGTKFLYSTGTMTDGITRGFLVDSASANKFG
                     FARRRYLGADLQLSLFSAIGMSSIRAEYIAGKQPGSKGSNVSVASASAPDYDTYLREF
                     NGGYIYCIQNIGQTKHQVVLKYDWYDPNIKIAGNQIRAKNNDGESTGLSAADIKYRTF
                     GLGWNYRFNSQVKFTLYYDIVKNEKTGIEGVNSTNDFTKDLKDNVFTFRVQYKF"
     gene            complement(62086..62847)
                     /locus_tag="PKI35_00555"
     CDS             complement(62086..62847)
                     /locus_tag="PKI35_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX42898.1"
                     /translation="MKALIVDDEQHGRLMIALMLTRLYPDAAYNLASGVQEARLMISQ
                     EKYDVLFMDIDMPETNGLSFLEMLRKEGIHTPVIIVTAYSKFEYTRKAIQLGVVDYIL
                     KPVVQDDFISAMERMQSFQTGIQEPHTRASEKSLSERITFRVLNGIMPVRKDELVYCA
                     ADSAYAVLYLKNGEQVRAFEALGDIEEKLPADTFCRCGRKYLVNLSMVRKLNLKRHSC
                     LLQYDKGVYEIELSDSGFAVLKQYFSGPAEEDKAD"
     gene            complement(62896..65967)
                     /locus_tag="PKI35_00560"
     CDS             complement(62896..65967)
                     /locus_tag="PKI35_00560"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNX42899.1"
                     /translation="MFRIAFILVFVSVTLFTGATAQIATRRFASVSYSTREGLVQSQI
                     RSLMQDSRGYLWICTKGGISRFNGQQFNNTFEGDASSIFKIIETGDLILALAKKSVYG
                     ISDNLVLKKLADLPDGFTFNLQTPLLLYVEKSNMAFFQVRDELLRTRFAAFNMNSNSI
                     EITPFLAADRILDVFRTHDEWFLFTESSCFVYKNGKFRTEFLNHRFSRFAFNQKLGAL
                     AGYSSSNHKFYRLTIFPGNIVREEPVKTKPSGIYPVLHQPIILDNENRIIFADSSDKQ
                     VKLIETDGLIKNIGIHGSSFNAGLCDREGNIWIGNNEGIHNFSNLKFEEVILTIDEGI
                     ADIGSILQDENGTMWFGCYGHGLWVLDRNGKLKKSGVVSGLPAVASDLNTFKEQNHIG
                     SFRDHEGRLYMNTENGIFIRDKQKLRLLPYKYLLINFENESGNNLYTGGDSKNGSGLF
                     IFDKSSWRLKEKYLWDYGAITCLAQDKAGKMYVGTDYAQGYLIDGKLVRENRKTAYKG
                     VLSMTADKLGNIWKATTNGLYLEFCNDSRIRKMAQLFRAFRIQHRPFCHLVNDIGYCI
                     PEVQILPEEIRGIVSCLYLHQDLLFAATTKGLFVIDLKKIYKGEPHFYSLYNRKNGYN
                     LLDPGQNGFFPDNDNHLWLTGYDRVICFAPELFKISTPVSPIIQNDITVTKDNLNWFP
                     IASVETPKGLPPSYNTLLFSFDGIGLSNPDQLIFRHRLVGYSNHWSDPSDQRVATFSN
                     LPSGKYIFEIICSTDKQTWSRVYRSQPVVITIPWYKNRVLNILLGLASIIQVTLLVLI
                     INKYITAKREENRKLSQLKLQALRAQSIPHFTGNALNSISFLILSGQKEDAGKYLNIF
                     NRYVDQTLMNIDNLMRTLHEEISYIELYLLLEKLRFGEKFDFIIKVDPAIPGSLPVPG
                     MVLHTWCENALRHGIKKLNNKGMIWVRAYRDGTNVIISVEDNGIGREAAKNINSGGHG
                     MGLQLVADQLRLLNEQGRWNFNYVVHDLYTPEGKASGSRFELVIPFPASLL"
     gene            complement(66315..67652)
                     /gene="gdhA"
                     /locus_tag="PKI35_00565"
     CDS             complement(66315..67652)
                     /gene="gdhA"
                     /locus_tag="PKI35_00565"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNX42900.1"
                     /translation="MEVEKIMSNLEKKHPGEVEYLQAVREVLESIEEVYNENPQFEAA
                     GIIDRLVEPDRILTFKVPWVDDNGKVNVNLGYRVQFNNAIGPYKGGLRFHPSVNLSIL
                     KFLGFEQIFKNSLTSLPMGGAKGGSDFNPKGKSNNEVMRFCQSFMLELWKLIGPETDV
                     PAGDIGVGGREIGYLYGMYKKLTHEHTGVLTGKGINWGGSLVRPEATGFGGIYFVKEM
                     LATKGQDLKGKVVAISGFGNVAWGAALKATELGAKVVTISGPDGYVYDPDGISGEKIN
                     YMLELRASNQDIVKPYTYEFPKAQFVQGKRPWEVKCDIALPCATQNELNGEDAKQLIA
                     NGCKLVGEISNMGCTPEAIEELIEHKILYAPGKAVNAGGVATSGLEMSQNSMKLSWAA
                     TEVDQRLHQIMINIHEACVKNGKQPDGYVNYVKGANIAGFMKVANAMLDLGVL"
     gene            complement(67803..>67999)
                     /locus_tag="PKI35_00570"
     CDS             complement(67803..>67999)
                     /locus_tag="PKI35_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42901.1"
                     /translation="VKVGEGNYEKGSGILTGFLPNGKKIRVVHYKNNLKHGDELIFNE
                     DGTIKDTITYRFGKVVKTAE"
BASE COUNT        18319 a        15956 c        14806 g        18818 t
ORIGIN      
        1 ctaattcccc aaaatgaagg attccctctc cctgactttc cctggatttt tcgcacaaac
       61 ggcagaaaga ttcggagatc gtccggctat ttcactgatt ggtgagaatc ccctgactta
      121 tgctgagctt gacgacaaaa tcaaatcgct gatggcatgg cttgaggcgc ttggtgtaaa
      181 acagggggac aagatggcca tactcagcat cagcattccc aactgggccg tcagctacct
      241 ggcagttacc ttcatgggcg ctgtagcggt tccggtccta cccgattttc accctgatga
      301 aatcaccaat atattggtgc attccggggc aaaaggcatt tttgtttcgg acgggctgag
      361 gtcaaagatt gaaaataccg gcaacgatga actcataaag atcaggattg aggattactc
      421 cctgtttaat ggtgaagccc cggccggttt gttcactccg ggagttattc cgcaagggca
      481 ttacgcgatt tcgcccgacg accttgccgc tataatttac acttcgggca caaccggaaa
      541 tccaaaggga gtgatgctta cgcaccggaa tatctgctat aatgccatac aatgcagcac
      601 cgtacatccc ataaacgaaa acgaccggtt cctttccatt ctgcctcttt cccatacata
      661 cgaaaatact atcggtctga tatatccgct gatgcgcggc gccagcattc attacctggg
      721 aaaggcacca tctccttcat tgctgttgcc ggccctgaag attgtcaggc ccactgccat
      781 gttatccgtt ccgctcatca tcgaaaaaat attcaggaat aagattcagc cggaattcag
      841 gaagaatatt tttctcagga tagcggtaca tatcccgttt atcaggaaaa aactcaatgt
      901 tgtagccggc aggaaactga tgagcacatt cggggacgaa ctccggttct tcggcatcgg
      961 cggatccaaa ctcaacaaaa ctgtggagaa atttctgatc gaagccaggt tcccgtacgc
     1021 catcgggtat ggtttaacag aaacctctcc tttgctggca ggcaccagcc ccggccattt
     1081 tcggctgcag tcgaccgggc cggcaatcga aggcgttgag ctttccattt ttaaaccgga
     1141 tccgaaaacc ggggaaggtg agatctgggc caggggtgcc aatgttatga aaggctacta
     1201 taaagagccc ggattgactg aagaagtcat gacccctgac ggctggttca gaaccggcga
     1261 cctgggagta ttcgaccgtg atggttattt gttcatcaaa ggacggtcga agaatatgat
     1321 cgtgcgtgcc ggaggcgaaa atatttatcc tgaagagatt gaatcgatca tcaataattt
     1381 ccgtcatgtg atcgattgcc ttgtcctcga tcaaaaaggt aagctggtcg ccctggttca
     1441 atttaacacg gatgagattg ccgaacgcaa caaatacctg aaggatgaga taaaagatta
     1501 cgttgaacat aaaattgaag agctccggct tgaacttcag ctttatgtca atcaccgcgt
     1561 gaacaaattc tcccagatac aggttgtgat tgttcagcag gagccatttg aaaagactgc
     1621 aacccaaaag attaaaagat acctttacag atagcccgtt tccatatttc taccggaacc
     1681 ggcccaatcc tcagtaatgc ggacggcggt cgcaggcttg gagttaattt cttctaaaat
     1741 caacatgttg cctttcgtag tttggagggc agtgctggaa acaaatttaa ccggaaaggc
     1801 taatcatcaa ttatttattc atcagcaaac aacaacacta tgggaagatc acaggaaacc
     1861 tttaacaaaa aagaagtcag gaacaaaaac cagaaaaaaa gaaaagaaaa agagcagaag
     1921 agactggccc gaaaaaacag cgacaagtca ggcaaccagg aagatatgat agcctatgtg
     1981 gatgaattcg gcatgatatc atcggtgcct cccgaccctg ataaaaagct ggaaattaag
     2041 actgaagata tcagaataag caccccaagg aaggattcag aatcggacgc cgacaaccag
     2101 cgcaaaggga cggtgacctt ttatgacgac gccaaaggct atggatttat aaaagattcc
     2161 gataccgggg aaagcatttt cgtgcacgcc aaaatattac aggaaaccgt gaatgccggc
     2221 gacaaggttt tttatgaagc agagaggagc ccgaaagggt attcagccat ttgggtgaaa
     2281 cacattgatg aaaagggcgc ctgatcagca tatttcccat gggttcttac agaagaacct
     2341 atactgctga tctgattcag attatcattt gatacctggt aaaaggattt gttgtcgctg
     2401 acagggcttg gccatttcat gataaatttt tatatcagat tgctttcgca gggcagtaca
     2461 ggataaaata tccttcttat tatttcgtaa gcttacccat gagcggccgg atttggtcag
     2521 ggacaaccag aattcccggg atttcagcaa tttcaggttg cagtgaacat gaacttccaa
     2581 taattcatac tggtttgcct gtaatacata aaacagggcg gcaaggtctt ttctgccggt
     2641 gatgtcgtgt gggcgctaaa ccgtgttccg ttcatattca gtacacaggc agatcaggat
     2701 tattacggta atcagggggg gggctatttc tttttagcgt aacgtgcctg gtaccgttct
     2761 tttgcttctt ccggatcggc gtctgccgga atgtagatca tcgtgttatg gtcgacaaca
     2821 acggcctgca atttgctcag gtcaggcgat gtggcattta cctgcttctt ttctttcatt
     2881 tagaatcctt ttaataatca tttcctgagc cggtcaaaaa acggaaatcc attcgtttac
     2941 agatcagtaa aatgctaaga tacacatttc gctggaatta tgatatattg aaaaatttta
     3001 aaattcgtac gtctttatat cccgcgattc ccttcttcct gaaactccat ttttttttaa
     3061 acaaaaaaga catctttccc gctgaaataa ccggatcaat ggatcaatcc atagtcgctg
     3121 catcctttcc gacgatccat tccaataaaa aatgccatcc gtcgctgcgg gagcgatgat
     3181 ggcatttgga ttacagatcc gtcaagctga ttaaaccaaa acctgctagt tggcgccggg
     3241 ttgttgtatc agggtatcgc tgttcattga ggccggcatg acaacttcct gttgggcttt
     3301 atctttagcg cggtgaggag cgtgcgggtg ccagcctacc gtaaacagca tgacaaagaa
     3361 accgacgatg taagcgacgg tgacatgcca gccatgttta acgtacttca ctaccgattt
     3421 ggcttcggga tacatgttgg aaagagcaac ccccgccgag gaaccgaacc agatcatgga
     3481 accgccgaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gttggaaaga
     3601 gcaacccccg ccgaggaacc gaaccagatc atggaaccgc cgaaaccgac ggcataagca
     3661 atgacacccc agtcatatcc tccttgttcg aggcagagtt tagtaagggg tatgttatca
     3721 aatacagccg aaacaaatcc cagcgaaaaa gccgtctgcc acgaggcttt tggcaattcg
     3781 tcgaccggca tcagcgacgc gcaggtcacc agcgcaagga ggaatattgt accctgaaga
     3841 gatttgggca cttcgttcca gggagccttg cggataaagt tcccgatgag tatggcaacc
     3901 caaacgccga tagccggaaa atcgagtgtc cagttggtaa tgatggcgcc gacaaggatc
     3961 atcaatacta tgatgagttt gccccagtca attttcacgc ttttggacag atttttcacg
     4021 ataggctgaa acctgtgttg ctggatagcc ccgataatcc cgaaaataag gaaggacgta
     4081 atcgaagcaa tgaaagcatg aataacatgc agtgggttaa ctccgtcaat ccacatcagg
     4141 gtagtagtag tatcgcccac aaccgacccg gcgccgcccg cattgctcgc tgcaacgatg
     4201 gctgccagga atccgatgtg aacctttccc ctgaatacaa ccattgctat agcgcctcct
     4261 atcatggcag cagcgatatt atcgaggaag gatgaaatta ccatgatggc aaaaagcaga
     4321 accactccgc ccctccagtc gtcaggcaac actttcggca accattcggg aattcccgat
     4381 tcttcaaaat acttggcaag gatagcgaaa ccgaacaaca aacccagcag gttcagtaag
     4441 gtgcgccact ccccttcttt atcaggagta ccggcaatgt gctcaggaag tgagaattca
     4501 ggaagaaagg catatttgag gatcagcaga acagccaacc cggccagggc cacgtacatg
     4561 gtcttatggt ggaatatggc tacgccgaca agagtgagtg cgaatatcac aaactcaagc
     4621 cgtatgccga aaaccagggg catcgcagcg gtggttgcgg caaaagacat aacaggggcc
     4681 agaaagaata aaagtgtcag gaaaaggcac cccagtgttt ttttcttttt catttttcag
     4741 gtgtatgttt tgttaacttt tgagagtcca taaggttaac cgtgagtcta aaagaaaaga
     4801 agggtatcag cagattcgct gtacccttct gtttatcaga tcatattgat caaaagcttt
     4861 atgagtatca gtatgattac tatgcctcca aaaaccagca ggaacggcag gaagccttta
     4921 tgcgttttag gcgcttcgtt ggatttgtta tctgttactt cgttcatttt gcttaaattt
     4981 aattatgtta tggttggcca tcaatccaat atttgctata cttccggcag gaagtgcaac
     5041 ggtatgaaat ggcaggtaaa atgagccggg aaagcggctt tagaaattta ttgataaaat
     5101 aatatcagaa atcagtctat atccgcaaat tatggtgcgc aatgaacagc gtgaatgttt
     5161 cggaaagaaa ggaatcccga taaacagaat ttcctgactg atgaaatgca ggagcttctg
     5221 ataccagggg tttaataatt ttccagtagc ataactgatt tttgtagcgt tccaggttat
     5281 cacggctgcc attggtgcca tcctggcccg gcagcacgaa catatcggtc cctgcaacct
     5341 gcaatgaaaa agggatcttc ccgccattgg cctgctttga caggttttgc tgcaactccg
     5401 tgcctgtcct gacttccatc aggaaaggaa gcaataagat gatgagacag caggtggtta
     5461 tcgatctcat ggaagaaaaa ttcgatgtaa aattattgat tttttctaca cgggttttga
     5521 tacgataatt tttcttccgt tttagaggcc gataaatttc aacatattaa atgtttaaca
     5581 aaaaaccggc aggcttttcc atgggaacta tccgggaaag cctgccggta tactcatttc
     5641 agcaaagcat caattgttgc attgatgccc atcgccatcc tgatggtgat gcaggcagtt
     5701 ctgcccgcta tccgtcaggc tgccatttaa aaaggccttt acggcatccc tggcatctcc
     5761 gttgcatccg cgcactacat caatacccca gtggtttaat acattcacag ccccatcgcc
     5821 tattccgcct gccagcataa gggtaacgcc ctggtcggcc agaacgcctg cgatatttga
     5881 tttacatccg cagccttgcg gcgacgggat aatactgaca tcagatattt cattgttttc
     5941 ggtaacggaa taaactccat agtattcgca atgtccgaaa tggtcgtcaa tctgatggtc
     6001 ggctgtgata ggagcagcaa tttttctgtt tttactcatc gttaatttag tttatgattt
     6061 cagcaatttt tcagaccaag actgaggcga tattttcaat tgcgccaatg acttccttat
     6121 cagcctgatg atagacagaa aatccttttt ctgcctcctc gccaacttcg gcaaccagtg
     6181 gtatcttccc cagaaatttt gtatttaatt ccttggcaag cgtttcaccc ccgtccgacc
     6241 caaaaataaa atatttctct tcaggatgcg actttggtgt aaaccacgac atattttcaa
     6301 cgactcctac gatcggaaca ttgatgccgg aattggtgaa taaggaagct ccttttcgcg
     6361 cgtcattcag cgaaatagcc tggggcgttg taactattat cgctcccgaa acattcagtt
     6421 gtttaactgt agaaagctga atatctcccg ttccaggagg aaaatcaaca atcataaaat
     6481 cgatatcgcc ccattgggtg ttttcgaata attgcaagat agcattagct gccattggcc
     6541 cgcgccaaat cagcccctgg ttttgtttta caaaaaaacc aacagaaatt atttttatcc
     6601 cgtatttctc aatcgggtac ataatctcct tctcccctac ggcgcttacc tctgggacga
     6661 catcctcgat tccgaacatt cttggaatgg aaggaccata aatatcggca tcgaccaggg
     6721 caattttaaa gccattgcgc gcaagagcga ttgacagatt agccgcgacg gttgatttac
     6781 ctacacctcc cttgcctgat gcaacgacaa taatttgttt cacaccaggc aaggtcttct
     6841 tctcatttac attcattcgt tttttactta aataattttc tttctatttc ttctttggca
     6901 agttcttttg tacaaaagaa ccaatcgtaa cagtgcatag atgatttatc ttccatcctt
     6961 tctttcgcca cgccacatga gccggcagtt ggaataatca catcatcgcc cggaagccaa
     7021 tctgcaggtg tggcaacaga aaaagcatct gctgtttgca aggcaaccag gacgcgatac
     7081 aattcatcaa aattccgtcc caggcttaaa ggatagtaga ttatagctct gatgattcct
     7141 ttcggatcaa taaaaaaaac ggccctgaca gcttttgtat tgctttctcc cggctgaatc
     7201 attccgtatt ttcctgccac ctccatcgta atatcttcga tcagcggaaa cctgacctcg
     7261 acatttttca tccccttgta ttcaattttt tcctttatgg tacgtaacca ggcaatatgg
     7321 ctgtataagc catcgacaga tagaccgatc aatttacaat tggctttgtt gaactgttct
     7381 tcccggcttg caaaggtcat aaattcagaa gtgcaaacgg gggtaaaatc agcagggtga
     7441 ctaaacagaa ttacccaact acctttgtaa tcatcgggga aattaatttc tccctgcgta
     7501 gtaacagctt tgaatgacgg agacatttca ccgatccggg gaatcggtaa gttcccttgg
     7561 tttaagttgt tttccatttt tttaaggttt taaggttaac acttgagtga attattaagg
     7621 attgaatttc gattatgatg atcaggaatt ccgcagtttt gatgctatca gttctgctgc
     7681 tttaatcaac ggataagcag caggtgatgc tgtcaggcac gggtgagtac caatctggga
     7741 gatcagaagg ctgttcactg tcattctgtt ctcaatcacg aggccgataa cattgatcaa
     7801 ttctcctgca ctggttccgc caataacttc ccccccgatg atcactcctg aattttttgc
     7861 cactatcagc ttcaccagtt gcttatggga gcccggcata tggcccggat gatggtcgac
     7921 accttcaaag actgatgtaa caacagcaat tccttcatcc tgagcccttc tttctgtcag
     7981 tccggctgtg ccaaatccat tttcaccgat agcggttgag taaataccaa tggtaccgct
     8041 aaaagtttta accacatgaa taccgaagag gttcattcct gcaaccctgg cttcggcaca
     8101 agccgtggag gcaagcatgg tagagacttt ttcccgggta aaaaaatcac gtttctgggc
     8161 gcagtcacca acagcaaaga tgtccttttc gtgagtcctc atatattcat ctaccgctat
     8221 gaatccgctt tcatcaacaa aaatgcctgc attaactgcc agcacagaat tgggatggta
     8281 ccccattgac aggattacag cttccgcatc aatagtatcg ccattttcaa gcactatacc
     8341 atcaactttt tcagaaccct taatttcccg aataccgttt cctgaaatta cttttactcc
     8401 acggttctga agaattgatg atattctgtc agcaatttcg gtatcgtaag ctgaagcaag
     8461 aatactgctt tccttttcga ccaggcaaac ctcatgaccg ctttttatca actcatccga
     8521 aaactcaaca ccgatgaagc cggctccgac aactacgatc ttttttaatc cctgcagttt
     8581 atagttcatt tcatcaagat aaactttatc ttttggtatt acgaatacgt tttccatatc
     8641 gcagcctttc aaccacttag gttttacagg ttgtgatccg gtagcgatga caagtttatc
     8701 atattgaatg gattcgccgg tagacagtac aacaatatta agctttttat cgataaatgt
     8761 aacttctccc actatagatt ctatacctgc tttttcaagc atattgtcca ccggcataat
     8821 attttgctgg ctactgtcaa gtgtgccgaa gatgtatgga atgccacaag ggaccaaaac
     8881 ttctttctgt ttcttgacca ggataaaact tttatccggc catgaagatt tacctgtaac
     8941 agcagcaacc ataccggcag cgctaccacc tatgatcaga acatctgcaa ttttcatttt
     9001 ttcagagttt tagtttaaat tttatttaat ccattctcca aatccatgat gatatcatca
     9061 gcatcttcga tacctaccga aaaccgaacc aggtcgtcgg taattccggc agcaagtcta
     9121 ttatccttgc ttacacctgc atgcgtcata gatgcaggat gctgaataag ggtttccacc
     9181 ccgcccaatg aaacggctaa caaagccagg tgaacattgt ccattaattt cttaccggcc
     9241 tcataaccgc caatcaatcc aaaactcatc atcgatccgg ggcctttcat ctgtctgcgg
     9301 gcaagttcat attgcggatg cgatttcagt ccagggtaat tgacccaagc cactttagga
     9361 tgggcctgca aaaattcggc aactttcatg gcattttcct gagcccgttc aacgcgtaaa
     9421 gaaagcgttt taacaccgcg aagcaccata aatgcctgag tcgggtccat attgcaaccc
     9481 atataaacca tagaatgccg tattttcttg taaaggtctt caccttttgt aacaataaca
     9541 ccgcccacga tgtcagcatg accattaatg aattttgtga tggagtgcaa tacaacgtca
     9601 gccccaagtt tcaggggatt ctgaagataa ggggagcaaa aagtattatc cacgaccagt
     9661 atcagattgt gctcttttgc aatttttgca caagcctcaa tgtctgaaat tcccattgta
     9721 ggattggcag gcgtctcaat atacaaaacc ttcgtattgc ttttcagcgc tgacagaatg
     9781 ttttcagttt cagaagtatt aacaaagctg gcttcaacat taaaccggga aaaatcattt
     9841 tccaggattc cgcgggttgg accgtaaact gcgtcactac agacaatatg gcttcctgct
     9901 cccagcaaag ccataaatac gctgctcacg gctcccatgc ctgagctggt ggcaatgccg
     9961 tcaaatccgt tttcgagctc ggcaatgtta ttctcaaatg ccctgatggt tgggttgctg
    10021 atacgcgaat agatatatcc gtcgctcttt cccgaaaagc agtcggcacc atgctgtgca
    10081 ttcctgaaac caaatgttga tgtctggtaa ataggcacac atgcgctccc gaattcgttg
    10141 tcaaaagccc ccgcatgaat cagtttagtg tcaaatcctt tgtttttagt gttcatttta
    10201 gtacttgttt attagttatt tgagtgttag tttttatcat tctgaggatg atttaaaatt
    10261 caatccctct gcgagcctca accccttcat tataataatg cttgatctcc ttcatctcgg
    10321 ttaccagctc agctctttct accagcgctt ccggggcatt tctgcctgtc agcaccaact
    10381 ccataggtaa aggctttttc tctatcaatt tgagtacttc ttctaatccg ataaggttgt
    10441 agtacaaggc tatacagatt tcgtcaagga ctactacgtc gtatttattg ccaatgatta
    10501 acttcgttat ttctttcaag ccccggcgtg cagcttggaa atcggcctca gtaggctggc
    10561 tgataataaa acattttaac ccatattgcc tgacttctat ttccggaata cgttccaggg
    10621 ctttaagttc tgaatacttc atccctttaa caaactgagc aataaaaacc tttttaccag
    10681 cgccggcagc tcgtatagag aggccaaatg ccgcggtggt cttacctttt ccgtcaccgg
    10741 tatatagatg aacgtaccct ttcatatttc actttttact gaaatttcgc tggtaataaa
    10801 cctgattatt gtaagttatt tccttgatca tgccatagct taaaagaaga ttcaattccc
    10861 tttcgctgga ctttgatttt tgaagcaatt ccagaacggc ttccctccgc atcggatgga
    10921 cagcggtgat gctaagtata tctttggtaa aatttcctga tgaagcaaaa gcgttacctt
    10981 catatccgat cagaaattct gtatgaagcc ctctttgttc aaaaattgca tacgctttaa
    11041 ccaaattacc ttcggctggt ctgtaagctt tattaaaagc agttggacgg gtcggaatgg
    11101 agataaaggc gatatccgga aaaatggaac ttataaaggc cgaagtttca taaatttctg
    11161 attccgaatc atttacattc ttaacaagca ttgtttcagt taccaattta ccgctgtaat
    11221 cctctttgaa aattaagagt gaatctttta tgtgttgaag gacgaggctt gaatgcggac
    11281 ggtttagggt tttccaggtt ttttcattca tcgtatcaac ctttaccgaa acatagtcgg
    11341 cttgtcccag ttcttctctt acctctttac tggtaatcag tgaagcattg gttatgacag
    11401 ctaccggtaa accattttgt ttcatttttt taatcaggaa gcccaggttc agatcgagcg
    11461 agggttcccc gttggccaca atggttacat agtcaggcat atcttcctca gaaagagcat
    11521 ccagatattt cctgacgcca tcggctaaca aatcaggatc gtaaaactca catcgactga
    11581 cttgtttctt gattgttttc ccgacctgac aataaagaca ggaatacgaa caatgcttat
    11641 aaggaatgtt attgatgccc aaacttttcc ccaatcgacg ggatggcaca ggtccgaaaa
    11701 tcagcgttct aaccatgttt tcttcagtct ttttgtatcg tcaaaccaat tatcctttac
    11761 attttccctg aacaaaccat accattgatt aaagcatttt ttttgaaatt tgtgaaagcc
    11821 cttctcctat aaggatgtac gagctactaa tcacctgttt gggaacctgt tttataacat
    11881 tgaaatttct tttagctggc cctgcttata gagttcatat gctttcctaa cagtgacatc
    11941 ataagcacca gtaaagattt tcatgttaag aggttttaaa gcttttgctg ccgtatcacc
    12001 cggaccattg cccgtaataa ggacgtcggg cctgaattga taaatttttc gggatgtagc
    12061 agttcccgcg ccatgggcct cattgttagc ctccagattg tttatcactt ccaggttatt
    12121 ggtttcttcg tccagtacca gaatgaagtc agtccttcca aacctgggat ctatttgtga
    12181 atcgagtgtt tcaccggtag cagtaaaggc gattttcatc agtttttttt agtttttcag
    12241 aaattcttta atagtagtcc agatatttac cagttgtcct gataaaggag aaccggtttc
    12301 agttatggtt aagcctttca ccatagattc cataaaaagg gaatcatacg gaatgtcggc
    12361 aatatgaagt atgttttgtt gtttcagata atcatgtatc tcttttgttt tagagatatt
    12421 gatgtcagat ttattgatta tgcatgccat ccttgcccta aaatgacgaa taagcgggat
    12481 tagccgttcc agatcatgaa ttccgctcac ggttggttca gtaatgatta gaacaaaagt
    12541 cgccccggcg agggatgaga ttaccggaca acctatgccc ggggggccgt cgactattat
    12601 aaaagggata tgctccgata cggcaatttt cctggcttca cttttaactt ttgcaaccaa
    12661 tttgcctgaa ttgcctgcag caatatcaag gctggcatgt accagtacat tatttatacg
    12721 ggtccgggag atataaaatt tccctgaaag ccgttcctcc attgagatag cctgtaaagg
    12781 gcagatcatt tggcaataac cgcaaccttc gcaggaaaca ggatctatca tataatcatc
    12841 acctgtgaga atgatcgctt caaatctgca tttcatgatg cataaaccac atttcataca
    12901 cttctcagga tgaatcctgg ccagatgccc gctgtaaaaa tcctctgaaa aatggaaatc
    12961 cggctgaagc agaaggtgca tgttcgatgc atctacatca caatctgcaa ttaccacctc
    13021 atttccggca agagaagcca gcgcagcggc aattgaagtc tttcccgtgc ccccttttcc
    13081 cgaaaggatt acaatctcat tcatggattc agtttcaatt gaatgatata atcggctatt
    13141 ttttcaagtg caggattcat cacatcacaa ttaccaaaca ccagcgaccc ttcagaatac
    13201 aacttcgaaa ttttgttgct gaatggtatt tttgccagga caggaatgtt accggtattg
    13261 cagtaatctt caagagaatc gtcccccatg tcactcctgt tgattacggc agcaaacggt
    13321 tttcccaaca ttcgcacggt actggctgca agcataaaat catgaatccc aaacggcgtt
    13381 ggttcagcaa ccagtatcac aaaatcagca tccttggtag cagctaccat cgggcatgat
    13441 gctccgggag gacaatcata caattgaacc ggtaaattcc cggtctggtt cctgactagc
    13501 tgaagcgttt tatggataag cggtaccgct tgttcttcac cgatttccag acaactatgt
    13561 atgaacctga gatttccggt tacaaattcc tttgtttcac caattttctg cggtttcata
    13621 cgaatggcgc ctgacgggca tagttcggag caggcaaagc aactatggca taattcgtta
    13681 aatacaacaa tggattcacc caaccgtaaa atagcatgga acctacaatg atcagagcaa
    13741 ctatcgcaaa acgtgcattg cgtttcattc caatcaggta taaatttata ttgtaccaat
    13801 gatttttgca atactccccc gatgaacaga aaatcatcag gctcctctac atccagatca
    13861 acaaggagtg tcggatagct tctgctaata caggctgcca ggttggttga aaggagggtt
    13921 ttccctgtgc ccccttttcc gctggcaatc gcaattcttt ttatatcagc cgaaacagcc
    13981 atacatcata cttttttcat ctattaaggt atacaggcat tcatcctgcc ggtgatcatt
    14041 tcagaaaaac cgggaaccgt gatattttca ctttattttg agtttcggtc agtctctatc
    14101 agacatcatc ctcgttgatg aactttgtgc aagttttcgg tgaacagata aaaagcagcc
    14161 agtaatagcc aaactgtttg acgatctcac ggttctcctt tattaattac tcttttgcgg
    14221 ccatatccat gatttctgcc aaagaagctg aagtattaac gaccagcttt acctccccgc
    14281 ttatcctctt ttgaaagcaa cccggcttca tgccgaaata ccctatacac atatcctgta
    14341 tttcaacgct taccaaacaa aggttgtttt taaatacaag gcaattcact tctgacagct
    14401 tgctttctgc agaaagaaac agacagaagc cataagcctt tcaactaaac tgtcgatcta
    14461 tctgatttcc agaactgctt tattaaatga agctaaaaga atcgtccgat tttttcctct
    14521 tgctgtgctg gtatcgagac tatttaccct gagcacaatc gtgatgatga tcgtcgtcgt
    14581 ggtcacaata attggccgta agttccagtt tgccagaaat aaaatcccgg acaatctctt
    14641 ctgcggtttt aattggagcc ccaacgaatg catttatttt ttgctcgtta aagagagata
    14701 tggcctgttg tcccatgcca cctgcaatta catcggtcac accaaattgt gcaatccagc
    14761 ggggatacaa acccggaaca tgttccggcg gtactacatc cctgatattc gcaatctgat
    14821 ggtcttgtat tgttactatt gcaaatgtct ggcaatgacc aaaatgggca cataatactc
    14881 catcttccat tggaatggcg attcttttat tcatctttac cttattatta ttaaatgttg
    14941 attctttatt tcagtaccca gacattatcg cttcccgcct ctgaaccggt tttgacagcc
    15001 cattccatat cggttagcaa ctttgcctaa gccatatcct cttccgtaga acggaacata
    15061 cgacgattcc ggttcatttg tttcggaagc cggtgttttt cttccgctac cagctccgaa
    15121 gttattacag cgtcccattc tgcgacctgt catgggcccc tggcccacgg gcccggtttt
    15181 atctaatcct ggcatattac ctccatttta ttaacagatt aacactagtt tttcccaact
    15241 cgtaaacgtt tccctgcgcc ttcaccaccg ccggacttat ggcgcttcgc ctgccctttt
    15301 cctaaaagca caagcttttc ttcctcggtt ttttcggaac attttcccag ctttctgccg
    15361 gtttgggtcc ctctgccatc gggtcctgta ccattaagct ttggcataac actaatttgt
    15421 ttgattgttc agtaattcta tgatttccct gatggtcctg ccacgatctt tcacgatgat
    15481 caattgtatt ttcaaactat cgagcaaccc tttgattttg atcccgaatt cgccggcaat
    15541 gattctggca acatttcgcg acaccatcaa ttgcacggca gacgtgccgg cgctttcttc
    15601 atccgttttg tatggattag ggatatattc caccgaacgg ctttcggtat catagataac
    15661 ataacaggaa catcttccga atttcagatc cacattcgat tcgggagtat ttcccgttga
    15721 agctatggca actttcatgg cgttttcaat ttatggtccc tgtctgcttt ttctccgcat
    15781 ccgggcattg catttcggac agatttgccg gttgcatggg tggacgtact gatgctctgt
    15841 ttcatatccg cagtccgggc atatttaatg gtcagcttca gtaacggcag tcgtttctgg
    15901 aatttcatct tcaaatgaat gaatttcatt gctgccgcaa agcggacaag ctgtaaccgg
    15961 ggtttcccaa tcaggattat taaaacaggc gttacaagtg tcgcactcaa accaggtgct
    16021 gtcgaagtaa acctttcccc cttcgatgat aatttgcctg ccttcgacca gcgcttcggc
    16081 tattttttgc ctggccgagg cataaatccg cgtgaatgta ggccgggata tgttcatgat
    16141 caccgatgac cggatatggt tatacctgtc ataatcacat aaccttaaag cctcatattc
    16201 ttcatataaa agggttacag gccctgaaac cgcttccgga acccccgggc cgtatggctt
    16261 taaccccttg atgacaggcg gatttactag ttttctcggg cgtttgaatc ttggcgacat
    16321 ataatgaaca tttgttcact acaaaattaa tgaacatttg ttcataaaga ttatttatat
    16381 catatttttg tagaaaaaat attcatgtat ggacgtccct attgttaacc aattccatgt
    16441 tgtaatgtcc ggagacctga atgaccgcaa ctgtttattt ggcgggcagg tattaaaatg
    16501 gatggatgaa acggcctatg tgtcggcctc gatatttgca aaagcggaag ttgtcaccgt
    16561 ttcggcagaa aagatcaggt ttctgaagcc ggtatttgcc ggaaatctga tcaatctgaa
    16621 agcggaagta ataaaatcaa acggattgaa aatcataatc cacaccattg cacttgcttc
    16681 agccgacaaa gcgctgacat gggatacaat agcagaagcc gaattttgtt ttgtcgccgt
    16741 cgacaagaac aatgccccga aacgaatcag ataatccatg aaactgtctg aagaactaag
    16801 aaaagatgta cgcttttcag aaggcatgaa agcatgcatc aactgcggca catgtaccgc
    16861 catttgcccg gcggcacatt tttatgatta cgatccaagg ctgatagcta ttaccgtgca
    16921 aagcggcgat gaaaaggaac tggaaaaatt gctcaagggt gatacaatct ggtactgcgg
    16981 tgaatgctta tcctgtaaaa cccgctgtcc cagggggaac accccgggtt atatcgttca
    17041 ggccctgcgc aacctttcga tcaaaacggg atatttttct ttttcggaaa gaggccgtca
    17101 gcaggtcagg gtgaaaagaa ttgttggcga aaatatactg aagcgtggct attgttttta
    17161 catcgacgag attaacacga ctgaccatcc cgaacaaggc ccgatctggg attggtaccg
    17221 gaagaacgcc gtttcaatcc tggaaaagac aggaggaaac tataaagctg aaggccccgg
    17281 aacgctcagg gatattcagc cgtccgacat ggaagaactt cacaggattt ttgccgaaac
    17341 aggcgcaata gaacaattta atgccataga ggattattgt gatgggaaaa agaaatgacc
    17401 gtgtactctg gcccgagtac caaaaaaata ttgccggcga tcattattat tttgcccgca
    17461 gttgcatacg ccagaatttt ttcccgggtt cggaagagtt gttccttcga atcctcaaag
    17521 aagtgctggg cctggacatt tttgacgacg ctcatcaaac cacctgtacc ggcattgcct
    17581 atcattcggg aatattaccg gttgaaacga caatgacggt agtggcgcgc cagttttcac
    17641 tgatgaacga tgccggatac cggaattttg ttccctcgtg cgtgacatcc ttcggcattt
    17701 ataccgaaat ggttgaaacc tggcagcatt ttcccgacac tcttgaaaaa acaaaagctc
    17761 aactcaaagc cgctaccggc agaagtttca gattgcctga aaacattgcg cataccagcg
    17821 acatcattta taaattcagg gacgaaattg ccgccaaagc caaatacagg ctggtgaatg
    17881 cccgcacggg aaaacccctg aaggtggttg agcatattgg ttgtcattat gccaagatat
    17941 ttccggcgaa aggcgtaggc ggagccgaat tccccagggt attgtcggga atgattgaag
    18001 cgtggggagg cgaagtgatc gactatccgg aacgacggca ttgctgtggt ttcgggttca
    18061 ggaattacat cattaaggag aaccgggggt attcgatatc caacacaaag agaaaactgg
    18121 actccatgga gccctatcat cccgatttta ttgtggccaa ctgcccgggt tgcacctttt
    18181 ttctcgatcg ctggcaatat gtgctggccg aaatggaagg caagacttat gatacggaag
    18241 gatacggcat accggtcctg acttatgaag aaatggcagg ccttgtgctg ggttacgatc
    18301 cctgggaaat cgggatgcag cttcaccagg taagcgtcga cagcctgctc aataaaatgg
    18361 gaatcggata caagtctggt gaaaaatacc tcggggcttc caaagaattt atcggcgagc
    18421 ccgaaaagcc atccgtttta aaagtaaaat gatggaagta ttggtggtta acgctacacc
    18481 cgggaaggaa aagtattccc ggaccctgaa cgtataacca gagtcgcaac gcttcatgct
    18541 tttcctgtaa ttctcagcgc aagattgaga agcctgctga agaaaaggga gttttatcat
    18601 gataaggtta cttgcatatt ttcctgacaa tgggtccgtt tagctcctat cacaaaatat
    18661 catatcttta tggtaagaat gcaactgtat tttcaataaa gatattcaac cagttatgca
    18721 aagaaaagcc ttacttattg tagcgctcgg cctggctatc acaccttttt cgggatttgg
    18781 acaacaaata tcagatacaa taaatttaaa aggagttgaa atagttggtt ccggcattcc
    18841 gaacaaaacc tcattatcaa aagtaaacct tgatcgggca gccatccccg atgttggagt
    18901 attgcttcgc tcggttcccg gtgttaacgg cataaaaaaa gggggttcct ccattgaccc
    18961 ggtcatcagg gggttcaggt ccagccagtt gacggtaatt gccgacgatg gtttgaaagt
    19021 tgagggcggc tgtccgaacc ggatggaccc ggttacttcg catatcgaaa ccgacgatat
    19081 tgaatccatc gacattatca aagggcctta tacgctccga tacggccctt cgctgggagg
    19141 tgtgatcaag atcaatacat tcaagccgca cccttatgaa actttccaga tccatgcaaa
    19201 atcacatact tcggtaaatt ccaatccgga aggaataagt cagtatttac tgattcacgg
    19261 cggcgggcag aagatatatt tcggcatatc cggaagctac aggaactatg gcgattatcg
    19321 cgatggcggc ggtaacctca tttcttcttc tttcaggaag tataattttt cgggtgacgt
    19381 gggttacaaa ccccatcctg accatgaaat cctgttgtcg gtgcaccgca atttcacccg
    19441 cgacatgaaa tttccggccc tgcccatgga tgaaatcagg gataacacaa cgcttttatc
    19501 gctcgattat catttcaggg taaggaacag cattttaaag caactggatt ttaaagtcta
    19561 taactccgat gtgtaccatg aaatggataa cagtttccgt cccgcgtaca gccaggttgt
    19621 tccaccttac aacggcctta tgcaggctgt gtcaaaagta aacgccgcca acaccggcgg
    19681 caggctggaa gcaaccctgg tttcgggaaa acaagagctg atcaccggcg ctgatgttga
    19741 aattacagga aaagacggtg gtcgggtgat gaccatgatc atgaatatgg gaggcattga
    19801 aacaaccacg gcaaagagaa cgaacctatg gaaagaagcg cgaaccatta actcaggact
    19861 ttttagtgaa tacacccttt cactccggga ttataaaatc cgcctttcgg caaggctcga
    19921 ttataatcat gcaggttcgg aagacaccct catccttatt gcaaatgata aaacctattt
    19981 taacgatacc cggtcggatt atttgaacat aagcgccaat gccggaattt caaaagcatt
    20041 cggaaaaggg aatcaaatca gcctgtttgt cgggcgcggg atgcgcagcc ccgatatgac
    20101 ggaacgattc ataaaattcc tgctggtggg ttatgataat tacgactacc tcgggaatcc
    20161 gcagttaaaa ccggaagtaa attatcaggc cgacctgatc cttgatctca atctgggcaa
    20221 agcaggcaaa tttaatatca gcggttatgg atccctggtg gattcctaca tttcaggagt
    20281 gattttaccg tcgactgccg ccacgccaaa aactatgggc gctgtgggcg taaagcagta
    20341 tatgaacacc ggaaaagctt acttcctcgg ttttgaaacc gggtacgcat cccctgtcat
    20401 caaaggtttc aacatcgatt tatcggctgc atatacatac ggaattcttg catcttcaac
    20461 acggaacatt tttgatgaaa acaataaacc tgtcggggtt gaagacgtta aaaacgatgc
    20521 catacaggaa ataccgccat tcgaatcagg aatcaacctg aactatgctt ttttaaaaga
    20581 aaaaatgcag gtttcgctgc aatcccggtt cgtccttgct caaaatcacg tttctttatc
    20641 gtattatgag aacaaaacgc ccgcttttat gttgtttaat ctgacttaca attataacct
    20701 taaccgtatg ttcatgattt cggcaggcgt tcagaacctt ttcaacagca cctattacga
    20761 acacctcaac cgtcgcattg tcggttctca ggataaactc aatgaagccg ggcgtagttt
    20821 ttacttaaac ctgaccatat cgttatgaaa caccttccac cggtaatatt gttgctgtgt
    20881 ttatttttcg cggcctgcga gccggagatc ataaagcctg aaaaagccgg cggaaaaatt
    20941 gtaatcgcat ttctccatca tgttgattcc tctcccctga ttaccgacag cctttgctat
    21001 gtgaatgaag ccgggaacgt ttacgaagtc aatgaattga aatatttcat ttcggatgtt
    21061 tacctcatca gaaacgggga acaaagtgta catattgaag atgagacagc gatacattac
    21121 accgatatcg acatcccggc caccctttcc tggaaggtgt acgataccgt tccggaagga
    21181 acctacgatt caatatcttt tatattcggc ctgaacgaag tacggaatgt atcctttgcc
    21241 tttgtcaatc cgcctgaggt gaacatgttc tggcctgata ttctgggagg cggataccat
    21301 tatttaatgc tgaacggaaa atggaaaaac cctgaaggaa acaccactcc tttcgatttt
    21361 cacctgggta tcggacagat ttattccggg acaaccactt ccgtggattc gatcacaggg
    21421 tacgtccaga attattttaa agttacgctt cctgtcgcag gacttgagat aaaaaagaac
    21481 acaacacgta ctatcggcct ggttatgaac atcgaaagct ggttttgcac tccccatgtt
    21541 tgggatttta atgtttttgg cggctatatc atgcagaacc aggaagccat gcaccgggcc
    21601 gttgagaacg ggcgtgatgt tttcgaaatt gggtcaatcc gataaaactg tctgtatgcc
    21661 ggtattcagg aaagttttca atttttacta tgaaggattc cggaatatga cggtgggtaa
    21721 gacactgtgg atcatcatat ttatcaagct cttcattttt tttatcattt taaagctgtt
    21781 ctttttccat gattttctcg gttcaaaatt caaatccgat aaggataaaa gcgactatgt
    21841 aatcgaacaa ttgttaacca aacctaaata accatggatg tatcactcgt cgactggtcg
    21901 agagcccagt ttgcattaac ggccatttat cactggcttt tcgttcccct cacccttggc
    21961 ctgtcattca tcatcgcctt catggaaagc atctatgtaa aaacgggcga tgaggaatgg
    22021 aaaaaaatca cgaaattctg gatgaccctt ttcggcgtta actttgccat tggcgtggcc
    22081 accggcatca tcctcgaatt tgaattcggg accaactggt cgaattattc ctggtttgtc
    22141 ggcgatattt tcggcgcccc ccttgcggtt gaaggcatca tggcattctt catggaaagc
    22201 acctttattg cggtaatgtt ctttggctgg aacaaagtga gtaaaaagtt ccatctcgtt
    22261 tcaacatggc tggtggctgc cggggccaac ctatctgccc tgtggatact ggtggccaat
    22321 gcctggatgc aatacccgac aggcatgcat ttcaatcccg aaacagcccg caacgagatg
    22381 ctcaatttct gggaggtgtt gttttcgccg gtagccatca acaaattcct gcatacggtt
    22441 acctccggct ttgtcctatc ctcagtgttt gttgtaggga tcagcgcctg gtacctgctc
    22501 aggaagcgga accagatgct ggcaaagcgc agcatgctga tcgcttctgt gttcggattt
    22561 gtcatgtcgc ttttcctgat cctgaccggt gacggttccg cccggttgct ggccaggcac
    22621 cagcccttga aattcgccgc tatggaaaac ctgtacgaag ggcaatccaa cgcgccgctg
    22681 gttgccgtcg gaatcctgaa aaatgacaat acagagccaa aaaagaagga tttcctgatg
    22741 aaacttgaaa ttccgggagt gctgtctttt atggcttttc agaatttcga tgcttatgtt
    22801 ccgggtatca gggatctggt tcacggcaat cctgaacaag gcatcatttc cgtcaacgaa
    22861 aagatcgcaa aaggaaaact ggcgatagaa gcgctgaaag agtataaaga agcgggcaaa
    22921 acaggcgata cggctaaagc cgattcggca tcggccgtct taaacagtaa tttcaagtat
    22981 ttcggttacg gttacctcga aaagcctcag gatgccatcc ctcctatcgg tccggcattt
    23041 tattcctttc gcatcatggt agctcttgga atgtggttcg tcctgctttt tatcctgacc
    23101 gttttcttcg tctttttcag aaagcctgag aagataaaat ggtatctgta tctttctgtt
    23161 ttaagtatcc cgcttgccta tctggcctcg gagaccggat ggctcgtagc agaattcgga
    23221 cggcaaccct gggtaatcca ggacctgatg ccggcatcgg ctgctgtctc aaggcttact
    23281 accggaagcg ttcagactac atttatcctg tttgcggtca cctttaccct tttgctgatc
    23341 gccgacataa aaatcatgag caaacaaatc aaaatcggac ctaaagacgg aggggaaaag
    23401 taatgtttga aaacctttca tatcctgctt tgcagcaata ctggtggatc attatctcgc
    23461 tgctcggagc tttgctcgtg ttcctgcttt tcgtgcaggg cgggcaaaca ctgatctacc
    23521 gtctgggaaa aaccgaaacc gaaagggcgc ttctcatcaa cgtcctcgga cggaaatggg
    23581 aattcacctt caccacgctg gtgactttcg gaggagcgtt ttttgcctct tttccgttgt
    23641 tttacagtac cagtttcgga ggcgcctact gggtttggat gctgatcctt tttgcctttg
    23701 tattgcaggc cgtttcctat gaataccgaa aaaaaccgga taatttcctg ggccatcgca
    23761 cttatgaggc cttcctgttt attaacggag cggcagggac tatcctgctc ggaacggctg
    23821 tttcaacctt ctttaccggt tctcagttct cggtgaacga aaacaatttc tcacaatggg
    23881 aaacagctac ccacggcctc gaagctgcta taaacatcca gaacctgtcg ctgggcctgg
    23941 ctgtattttg cctttcgcgt gtgctggcat tactctattt catgaagaat gtgaaccatg
    24001 acgaaattat cgccagggcc aaaaaacaac ttctggtcaa tgccattccg ttccttgtgt
    24061 ttttcctgct ttttatcatg ctgcttatgc taaagaaagg gtttgccgtt gatccgctta
    24121 ccggacttgt ttttatggaa cctttcaaat acctgcataa catgatccaa atgccggttg
    24181 ccggaatcct gttcctgacg ggtgtagtcc tggttttaac cggaatcttt ctgccggttt
    24241 tctctttcca gtccttcgga cgtcagggca tactttttgc cggaacaggc tctatcctca
    24301 ccgtttttgc acttttcctc attgcaggat tcaatcacac ggcattttac ccctcagtgt
    24361 ttgacctgca aagttcactg accatttata acagttcctc gagccgttac accctgacgg
    24421 ccatgagcta tgtgtcgctg cttgttccgt ttgtcctggc atacatcatc tatacctggc
    24481 gcgccatcaa caggaagaag atcgatatct cagaactgga aaacgaaagc cacacatatt
    24541 aacctaaaaa ttcacgctat gataatccag attatttggt tcttcagtct gcctgtcgtc
    24601 atattcttaa catacaggct tgtattggcc gctctcagaa aatttgagaa aggcaataaa
    24661 taaactgttc atctgttaat gaaaaagagt tgagataata acatgatgat ggtcaggaag
    24721 ctaaaagcga ttgttgcttt attattgatc acggccctgt tctggccttc ggtcataaag
    24781 cttgaccatc atcatttcta tattccggtt ccgggcacag gagaacagtt aacagttgtt
    24841 catgaaaaat gccctgcatg cagtttcgaa tattcaatat ttatttcaga tagcggggtt
    24901 aaaggacctg aacgtgccga agttatttgc gagcggttct gcttcatcct tcccctgcac
    24961 attctgtttt cgcctcggta ttccttcttc ctgcgcgcgc ctcctgagaa gatgatctag
    25021 cgcttcggct gccaacgggc agctattcac catttcaata attttattaa aataaaccat
    25081 gaaaagcata tttataatat gcctgctggt attgttgtac aacaccggca tggcacaaaa
    25141 taccctgtac ggaaaagtca ccgatcagga taatactcct ctggcaggag ccaatgtgta
    25201 cctgcccgaa ttgaacaaag gcacggtgac tgacgcaagc ggaaattaca tcctgaaaaa
    25261 catgtcgaac ggaaaaatta aaattcagtt ttcatacctg ggttatacct caaggattga
    25321 gaccattgta cttaaccatc aggatacaag ggttgatgtt tcgatgcata aaaccatgat
    25381 tgagactgaa ggcattgtcg tgaccggagg atacagctca acacaacacg aaaatgctgt
    25441 taagattgat atcctgaaac tcggtctgga gaacctgaaa tgtaccccga atttcatgga
    25501 atcacttacc agggttcccg gagtgaacat gatctcgaaa ggaagcggaa ttacaaaacc
    25561 tgtgatcagg gggctttcga tgaacgatat acttgtactc gataacggcg taaggtttga
    25621 aaactaccag tattccagcc atcacccgtt gggaatagat gaaactggaa ttgaaagcgc
    25681 ggaaataatc aaagggcctg cttctttact atatggttcc gacgcaatag gcggtgtaat
    25741 taactttatc agggaaaagc ccgcatcggt caacagcatc gaaggggatt ataacgtcaa
    25801 ttttaattcc aatacgcttg gcatagccaa caatctcggg ataaaaggca cgtccggccg
    25861 gtttttcgga ggaataaggg ccggcatgaa atataatgcc gattttctgc aaggcggagg
    25921 ccaatttgtc cctaattcca ggttccatga atattcgata aagacaaatg cgggatacac
    25981 aggaaaagcc ggtacgatca ggctgttcta tgattttatc aggcaaaacc tcggccttgt
    26041 tgaggaagag gccctggaag ccataaagga aagaggttat aaatgtgaga tattttatca
    26101 gcaactgaat acgcacctgt tgtcttcgca aagcaagctc ttcctgggaa gaaccaaact
    26161 cgacctgaat gcatcctttc agaataccgg actcctccat gttggcgaag aagatgaata
    26221 tgaaatacac atgaaactgg cgaccttatc ctatgaagcc aagttatacc tgccatccga
    26281 cgagtcttcc gaatacatcg tagggtatca ggggataaac cagttcaaca ccaatctgca
    26341 caaccgtgaa accattttgt tacctgacgc gaaaacaacc aatcagtcgg ttttcggcct
    26401 ggcacaacgg acatttttcc gtaagatgaa aacacaggca ggagtaagat acgacctgaa
    26461 atggatcagc accaattctg tcgggctacc cggaaacagc agttaccgcg aagcccttga
    26521 ccggaattac gggagtttca gcggctcgct gggcgctacc tataattttt ccgaaaaatt
    26581 tctgctcaga ggtaacattg ctgccgctta ccggactcca aaccttgccg aactcacctc
    26641 gaacggcgct catgaagcgc gctatgaaat aggggataaa aatctggggc ctgaaaaatc
    26701 attggaattc gacctgagcc ttcactacca tatcgaaaac cttaccattg acctggcagg
    26761 ctatcgcaac aacatccgtg atttcatttt catctcgcct acaggtgaaa ttaccgggaa
    26821 cggacttccg gtttatcgtt acctgcagca gaactcctac ctgtatggag gagaagccgg
    26881 cttacattat catccggagc atctgaaatg gttacatatc gaaaccacgt tttcgagcgt
    26941 aacaggcaaa cattctgacg ggagttacct gccgtttata ccggccaaca taataaacct
    27001 gaacctgatg attagaaaaa acaaactcgg gttcatgaaa aacgcctcgg tatcggttga
    27061 tttctccaaa acatttgatc agagcaatcc ggcgcctgat gaatcggcaa ctgacgggta
    27121 tttcctgact gatatctcgg caggaggtga attcagcctt caaaaacatc ctgtcatggt
    27181 aaggctcggt gcgacaaact tatttgacgt ccggtacact gatcacctgt caaccctgaa
    27241 ggaagtcaat ctttttaatc ccggaagaaa catttcggtt tttctcagga ttcctttcag
    27301 tagccaacta tacaacaagt agctaacgaa gacatccctg gtgttgttaa caaccggcca
    27361 tttcttatct gaaaaaatca ctaaaacacg caaaaaagtt aatccattaa aatactgaca
    27421 atacccattc gcgtcaaaca gacaggcatc ttctccctgc aaagtatttg gttaaaattt
    27481 cgcttcagga gttcgttagg aaaaataatg aacggatgcc gctggttttt tcaatctcgg
    27541 gtgtccaata tacataacac caaaaaattc atgctatgaa aaacacagtt agactatttg
    27601 ccgccatggc cctgaccgca ttttttatca cagggttgca ggcacagacg acatcggctg
    27661 atcaagcaaa aaccagacag aagtcaacgg cgataaaccg tccggctttc gttgacaaaa
    27721 acaatgacgg tgtatgcgac aattatagtt ccggaaggta tcagggacgc ggcgccaatt
    27781 tcgttgacaa aaacaatgac ggtgtatgcg acaattatgg ttcgggaagg tatcagggac
    27841 gtggcgccaa ttttatcgac agtaacaagg atggcatctg cgataaccgg caaggtgcga
    27901 ccggaaaatg gaactccggc tgctctatgg gtaacgggaa ccgccaccgg catgggaatg
    27961 gtcattgttg cggcagcctt aaacaaaact gatgataaag ttgtaaagaa atagttaata
    28021 atttgtaaac cacgttttct aaaaggaggt tgaaatattt atctttgagc atatattgat
    28081 ggtgcggcat agtaaaacct gcatccattg gtaaccgggg atctgtattt caacctctta
    28141 ttacgctctg agctgatgac cgaccaggaa ttaatcacag gcatccgaaa ccgcgacagg
    28201 caggtcatca ggcaccttgt gggaacttat cacaagcagg ttataaaaac tgcatataac
    28261 ttcaccgaca atatggatga cgccgaagac attgcccagg atgtttgcat cgaaatactt
    28321 gaatcggtaa agcatttcag gggtgattct tcgttgtcta cctggattta caggcttacc
    28381 gtcaacaaat cgctgaactt catcaggaaa aacaagcgca agcaacttgt aaggcaattt
    28441 gaaacctttt tcaggattgc agctaatggt gcctggaacg gaattccgga accttattcg
    28501 actgaagacc ctgtgcaaaa gaatgaaagc cgcattttac ttgacgatgc cgtcagccgt
    28561 ttgcctgaaa atcaaaagac cgcctttatc ctgaataaat atgaggaaat gtcgtacaaa
    28621 caggttgccg aagtgatgaa cctgagcctc tccagcgttg aatcgctttt acaaagggca
    28681 aaacaaaacc ttcagaaaag cctgatagct caattctcag aatattccaa aaccggaaca
    28741 tcatgacaga ttgcaggtta ttacacgaaa acctttacgg atatgctgcg aaagagcttc
    28801 cggatacgct gatggatcaa ctggatcagc acattgctgt atgccttgac tgcaaacaac
    28861 tcctggagca gttcaatgac gtactaaatg ccatcgggga acatgtcggg gctgaacctc
    28921 tcccctatgc cggttcacgc cttttgcacc ggctggaagc agcccttgaa atgcctgaaa
    28981 aatcatcttt cgttttttca aagagcatcc ttcaaccggc tctcatcagc cttggtatgc
    29041 ttcttgcact tgcgatcggt tttctcatcg gaacggaagg agccgggcgt tatgctgcct
    29101 ataacgaaaa tacgatccag gaagttcgtt cggacctgaa cgtaccagat tttatgaccg
    29161 acgaactgct ggaattcact gaataaacct tcaagccatg gattttttga aaaagaaccg
    29221 cattgtattc tgggtattga tattcctggt cttcgtgaat atcgcagctc tggctaccca
    29281 ttttatcagc ttgcagaaac atgctgctga accggttagc tcacctgaag ggagaaaagg
    29341 agttgcaatc gggagagagt tatctctgag ccctgaacaa ttaaacaaag taaataagat
    29401 caatactgcg taccggttct ccagtgagcc catcataaca gccctcaggg ataaaaaggc
    29461 ggcattgctg gaagaactct cgaaagacac atcggatagt ctccggattg cagttctggc
    29521 tgaagaggta agccgggagc agaataaact gcaacaggca aacattaggc agttcctcga
    29581 tttgaaaaaa gtttgtactc ctgaacaaac tcaaaaactc tcaggcatct atgccgggtt
    29641 ctatggctgc gccggagccg gacgagggca agggtgcggg aacggcaacg gaaaaggata
    29701 ccggcaccgt cacggctgga aacaacagga taccattagt aacggagaat aataaaagac
    29761 gatataactc tgcttccggt tatgaatacg aaaattcaaa aaaatatagc catcagcgac
    29821 tctggctttc tgtttaatcc cactaccggc gaatcgttct cggttaaccc catcggactg
    29881 gatattatta atctgatgcg tgagggaaga agcgaatctg aaatctgcac aacccttctt
    29941 gaaagttacg aaaccgaatc tgtcatgatc gagcgcgacc ttcacgattt cattcacatg
    30001 ctcaggcagc atcaattact cgaaaacaat gacggagagg atcattaaca tcggcgtcac
    30061 aggcttaaat gccatcgaca gccccggccc gggcgttccg gtcataagag gattgcgcga
    30121 agcgacatct ttcaaaaccc ggattatcgg gctggcttat gaaccgctcg aaccgggaat
    30181 atacctcgac gcgcttgtcg ataaaaccta cctgattcct tacccttcag ccggaacgga
    30241 tatgctgctg gcccgcctgg aacatattca gtcaattgaa aaacttgatg tcatcatccc
    30301 gaattttgat gccgaactat attccttcat aaagttggag cctgctttga aaaaaatcgg
    30361 catcagcatg ttcctcccta cgatggaaca atttgaagag cggcacaagg taaacctgaa
    30421 tactttcggc gaaaagcaca atatggatgt tcctgaaagc aaagctgtca ttaatcttgt
    30481 tgatgtggtg aaaacggcca aagagatggg attcccggtt atggtaaaag gaaagtacta
    30541 tgacgcatat gttgccgcta ccacggatca ggcccggaat tacttcgaaa aagtaagtgc
    30601 aaaatggggc ttgccggtaa tcctgcaaaa gtttgtaaaa ggcaccgaac tgaacatgac
    30661 cggactcggc gacggaaacg gaaacacgat tgcagccgtt ggcatgagga agcagtttat
    30721 cactgacaaa ggaaaagcat gggccggaat tacaattcat gattacaggc tcatggaact
    30781 cacgcgccat ttcattacca caacaaaatg gcgcggcggt tttgaactgg aaatcatgaa
    30841 atcatccgat aacacctatt atctgatgga gataaatccc cgaatcccgg catggatata
    30901 tctggccatc ggcgtcgggc aaaacattcc ggaagctttg gtaaatctgg cgcttggccg
    30961 gaaagtagag ccttacgaaa cctatgaagc cggaaaattg ttcatccggt attcctatga
    31021 tatgattgtt tcgagagaac ggtttgaaga actgtcagtc aacggggaat tggaaaacga
    31081 cattgaaaaa ccgtaatcta caattacact ttctatctgc attactatgg ccagacaatt
    31141 ttatgaacga ccgataatcc ggaaactgga aaccggtatg ccaggcaaat tcggcatgcg
    31201 taccgactac cctcctacag cgcaaattga cgggattccc gtaaaatcgc ttctggagaa
    31261 gtacggttcc ccattattcg tcctttcgga agacaccatc cgcactacct ttaaaagggc
    31321 tcagcgtgcc tttacgaacc gttatcccaa ggttcagttc gcatggtcgt ataaaaccaa
    31381 ctacctggat gccgtctgcc ggatatttca ccaggaaggc tcatgggctg aagtggtatc
    31441 gggatttgaa tatgataaag ccttgcataa tggagtggat ggaaagaaaa tcattttcaa
    31501 cgggccgggc aaatccggat cggatctgaa aaaagccatc gaaaatgatt ccctcatcca
    31561 tatcgatcac cttgacgaat tatatctcct gattagtatt gccaaaccgg ataaaaaacc
    31621 aagagttgca atcagggtga atatggatac cgggatttac ccccaatggg accgtttcgg
    31681 gtttaattat gaaaacggcc aggcctggga tgccatcaat aaaattgtaa gttcgggcaa
    31741 acttgaactg acagggcttc acacccacat cgggaccttt attcttacgc cgaacgccta
    31801 tgctgttgct gcaacaaagc ttgccgacct ggcttcggaa atcagccaca aattcaattt
    31861 aaagattaag tatatcgaca tgggtggcgg attcacgtcc cgcaacaccc tcaaagggtc
    31921 ctatctgcct gggccggatg taaatccatc ttttgatgat tatgccgaag ccatttccag
    31981 tgcattgcta aacagccggc tcaatgtcga tgctttgccc ctgttgatac tcgaaaccgg
    32041 ccgggcactg attgatgaag ccggttacct ggccggaagc gtaatcgcca ataagcggct
    32101 tgcttcagga aaaaaatccg tgatcctcga tatgggcgta aacctgcttt tcaccgcatt
    32161 ctggtacgac cacaagatta cgccggccca gcctttttcg ctttacaggg aagacacggt
    32221 gctttacggc cccttgtgca tgaacattga tattataggg gataatatta gcttcccgca
    32281 actcagtgtc ggcgacggcg ttgttatcca ttcagtggga gcgtataata tgacgcaatg
    32341 gatgcaattc atcaccctga gacctggaat tgtcctgata gatacaaagg ggaacacgca
    32401 tgtgatacgc gacaaggaat ctatcggtac aatcattgag aatgaaaagg taccggaaca
    32461 cctgaaaaat ttctgatact gtcacccttt gactagctgt aatcataata tcaaatgaga
    32521 tatctgcaaa ccgacaaact gaaagagagc cttaccgatc actttgccgg ctttcttaac
    32581 agctatgcca tggtgttctt ttccaaggac cgtctgtttg ccggcatcct gattctcgtc
    32641 acctttttcg atccggttgc cggattaagc ggactcgttt cggtgctttc cgctaacctt
    32701 gtctctggca ttcttggttt aaaccggttc aatgtccgtt ccggctttta cggtttcaat
    32761 gcattgctgg ttgggttagg cattggcctg tactaccagc tcacccttga actcgtcatc
    32821 cttctggtgt tctcttcgct gctcaccttg tttatcaccg tcctgctcga aggagttatc
    32881 ggcaagtaca aattgccata ccttaccata cctttcctgc tgactttctg gctggtgacg
    32941 ctggctgcca gatactatgc cgaacttcac atcagcgaac gcggtgttta ccagatcaat
    33001 gaactataca gcctgggagg aaagcgaatg cttcaactga atgattggtt cagtacattg
    33061 ccattggcgc cttccatcgt cacctatttc aagtcgttat cagccatctt atttcaatat
    33121 cacctgttca cagggatgct ggtagctgcc ggcttgttat ggtattcccg catcgctttt
    33181 ctgctctcgt taacaggctt ttattcggca tacctttttt atatggtgat cggcgccaac
    33241 tttaacgagc tcaattacag ctttatcgga ttcaactata tacttacagc catcgccatc
    33301 ggaggatttt acatagtatc gtcacgcaca tccttccttt gggtcatcct gcttacgccc
    33361 ttgatttcaa tcctcgttac aagcacttcg gtgatcctgg gtacctttca gctttcaacg
    33421 tattccctgc cgtttaacct gacggtccta ttgtttttat atacattgaa attccgggac
    33481 aatagacaga ccggccttca tcttgtaagc cttcaggagt attccccgga aaaaaatctt
    33541 tatacgcaac ggaacaatga aacaaggttt cctgaaagtt cctattatcc gtttagcttg
    33601 ccggtcatgg gcgaatggaa ggttacgcag ggtcattcag gtgaatttac ccaccgggaa
    33661 ggctggcgcc atgcctggga tcttgaaatt gccgatgata aaggttctgt tttttcagga
    33721 aaggggctga tgcccggaga ttattattgc tacgaaaagc ctgttgttgc ttctgccaat
    33781 ggttgggttg aagagattct tgataacatc cccgacaacg aaatcgggca ggtcaacctt
    33841 cagcagaact gggggaatac cattattatt cgacattccg atacacttta tacaaagata
    33901 tgtcacctga agaagggcag ctttaaggta aaaaaaggcg atagcgtaaa aaaaggcgaa
    33961 gcagttgcct tatgcggtaa ctcaggacgg tctccggttc ctcatgtgca cttccaggtc
    34021 cagtccactc cttttattgg atcccttacc attgattatc ctctgagcca atatctgctg
    34081 aatacagaga aagggtatga aattaaatca ttctcccggc ccgttaccgg agacattgta
    34141 tcgaacctta accccaattc atgccttgga aaggcctttg atttcgttcc gggccagacc
    34201 attgaatttt cggtgtcggc tgatggatgt gttccttccg tcatacgctg ggatgttatg
    34261 gccgatattt ataaccgcac ctatatccaa tgccggcaaa gtggttctaa agcttacttt
    34321 ttcagcaaca gcgacatttt ttatttcacc tggttcgaag gaaataagaa atcgttgctt
    34381 tttgcttttt acctcggcgc atacaaaatt gcaaagggct actaccggaa ccttaccatc
    34441 cgagatatat atccggttaa agtatgcggc aatggcttat ttaaaatgct ccaggatttc
    34501 gttgccccgt tcttcttatt tctgcacaat gagtatgaaa tgtgttactg ttccatggag
    34561 gacgaacttg aacaaaggag cataaagatg aagtcgtcag cccgcaagca aatatgtaaa
    34621 aggtcatcat cgatggcgga atttgaattt ttaattgata acgaccgcgt tgaaaaattc
    34681 gtcatcactg aagggaagca tatcaccacg gccatcagga gtattaatcc acagaacttt
    34741 taacgccttc ggacatgaaa cgaccagaat ttttccgcgt taaattgccg gctatgatga
    34801 cagcgatttt gattctgtta tctgtcagag gatacagcca ggagaaagat ttccggtatt
    34861 acgacagcct gagctatggc ctctatacgc taaaagactg gaaatcactg atcgccgttg
    34921 cagaagaagc tgagtcaaaa ggctatgact attactacct gaacatgcgt ttaggtattg
    34981 cacattatga aatgaaggat tatcgttcat ccataccata ctttgaaaag tccctggaat
    35041 acagcaccga cgaaccggca gccgttgaat atctttattt cgcgtacctc gaatccgggc
    35101 gcagcggtga tgcatcgatg cttgccgcga aatacaggga gaagcttgcc tctgtccccg
    35161 gcacaggatc aaaaattctg aattttattt ataccgaggg aggttataca ccggatgctg
    35221 cttcgcgcct tacaccttcg gcattgatag gaaacgacag catttacggt gaagaagatg
    35281 catttaaaac acaatcctat ttccacttgg gaatccaatt acaaccgctg cccttcctga
    35341 aagtatatgc ctccggctcc atgcttggca tcggcaagca aaagcatttt gcattcgcca
    35401 attataatgc ttcagggaat tttctgtcgg taattgatac ggtctttcct tattcataca
    35461 accagaagga attttatatc gcagcttctc tttctccata ccgtggcttt accatcactc
    35521 cggctcttca tgttatgcag ggagatcctt caccggtaaa ctgcatttat tcggattata
    35581 catactcctt cagcgaggac gcatacagct acagccatta tgtgttttct cttcttctga
    35641 ccaaagagat gagaaagttt accctgggag tgtcaggttc tgtttcaaaa cttactccaa
    35701 aaggcaggca atatcaggca tctgcttcgt taacgtggta tccgaagggc aacctggata
    35761 tttatacaac ctcaatcatc agcgtcctta gggagaataa aaaaacacgg ctaatctatg
    35821 aacagactgt gggcttaaag atggccccta aactatggct ggaaggttct gcaacggtcg
    35881 gggacttagc ctatttcaat gaaaaaaatg ctttcgtggt ttataacctt cctgaacata
    35941 tcgtattcag aggtggagcc agtctgatat atatgctgaa ccgtcatctg gatgtatcat
    36001 ttatgtaccg gctgtattcc cgggagaacg attatttgta ttacagttac gatccggacc
    36061 gcgggaggct gatttcccaa acccggaaga atgcctatgt aaatcaaagc ttttttggag
    36121 ggctgaaatg gaaattttaa agaaaccggt aacgtggata gttctgcttt ttaatctgaa
    36181 cataggaatt attcatcttt atgcccagga ttttcagaaa ttgcagaatg catttattgc
    36241 cagctattct tacgaaaaca ataagaatta cgcgaaagcc atagatgcct tgaaacaggt
    36301 ttatgacggc cagtcatatg aaatcaatgt tcgtctcggc tggttatact attatgcagc
    36361 caactatcct gaatccatca aattttattc aaaagcgatt caactgaaac cttactcggt
    36421 cgaggccagg tttggttatg cattgccggc ttcggccatg ggcaactgga acgatgtgct
    36481 gaagaaatat tttgaaatac tggaaatcga taaaaataat tacacggcga actatcgcat
    36541 cggtcttatt tactataacc ggaaagatta tgcaacggca tacaggtact ttgagaaact
    36601 tgcaaacatg tttccttacg aatacgacgt cacccacatg tttgcatgga ccaattaccg
    36661 gctcggcaag ttgcgcgaag ccagggtgct ttttcagaaa accctgctta tcaagcccgg
    36721 cgatagttcc gcagcggaag gccttaagct gataaagtaa agcgacgtac cgggcagacc
    36781 ctgaaggttt taaaaacctt cagggtcttt cccccccccc ctgaaatatc cggaataatt
    36841 accatcgctg acaatatttc atctccccct gtaacaaaag ggtttatttt ttcgtcagga
    36901 tatttataaa caccaaaaaa tcatgaaaaa aatcagtcaa attttcttac tactattggt
    36961 tgccgccggc ttaccatttt catcagttgc caatccggga aatgacgggg ccatttcagg
    37021 aaacggaaag gttacaacac aaaaccgtac gatcggttca tttcacgctt taaaagtcag
    37081 cggtggaata gacgtggatc tctcgcaggg aaacgatata aaattgcagg ttgaagccga
    37141 tgaaaacctg atcgagaata tacagacggt cgttaaagac ggcgttttaa acatctaccc
    37201 tgaagaaagt gtacggaagg caaaagtcat gaagattcat ctcactttca gggacataga
    37261 tgccattaat gcaagcggcg gttgcgacat cacctcaacg caaaaactgg atttcacgac
    37321 gctgcagact gatctgagcg gcggctgcga cataaagctt gaactgaagg tcaataacct
    37381 ggtaagtaaa cacaacggcg gttgcgatgc taccttcaat ggtgaagccg aaaactgtac
    37441 cctgaaagta tcaggcggat gcgatgtaaa agcatcggat tttcacataa aaaactgtag
    37501 cgttgatgca tccggcggtt cggatgtatg ggtatatgca actggcattc ttgatttgaa
    37561 agcaagcggc gccagtgatg tcacttatgc aggctcgcct tcgaaagtca ccaaaagcgc
    37621 ttccggcgca tcggatattc actgcaaata gcatagcatt aatctgttcc tgaatgccgt
    37681 tgagatcagg aggcagagga acaaaatatg agaggctgtc ccgatgccgg gacagcctct
    37741 catattaatc cacatgtatt agctcttttg taatgaattt actatggctt accggagaat
    37801 caagcccggc ggtataaagc atcccggctt tgccaggaat catttttaga ttttaatgat
    37861 cttagcccgg gagccgaggc tcttcaggat ataaacgccc ggttttagat ttctgatatc
    37921 gatttcattg gccccttcct gcaagtatcc ttctttcaac agttttccgc tgaaatcata
    37981 aagcccgtat ttactgccgg cgtccccttc aactactact cttacctttt catgagccgg
    38041 attaggaaac acctgcattt tcccggtccg atccttgtca ggtatggaac aaaaaatacc
    38101 gggcagctta atcaattgat tgacggacgg ggtaattgat gccatattat cgaggagcaa
    38161 ccggactgat gaaagattat ccccgtcgta gtcgcgggca aaggtcacac aataatccaa
    38221 aggttgctgc tgccctgctt cgaaggtaaa aggcccggat atggcaagca ttctggtatc
    38281 taacggggta ttgccggaag ttacttcagt ccagtagtaa ccgttttggt tataaccgcc
    38341 attggggagt gcaccgttcg tgccccagtt gcataacgtg tcggaatcgc ccggataaat
    38401 aaacctgcac tcagggccga cagcccctgt tgaaagattt ccgcttccgc catagttcag
    38461 gaaagtcccg tcgctccacc tgttttgcat acaattgtaa tattcagtgg aggcattcgg
    38521 ataaatatca taattcatcg gatcgtttat cacacaagta cttcctgtca atccaaaccg
    38581 ttcattgtcg ataatcccat ccccgaaatt caacccgtta atatcgtaat tgcattcgcc
    38641 ttccggatta tcaattccat catttgccat aaaagggccg cccaatatcc tgagtccgat
    38701 ggcaggggga tgttccccgt atgcctgcgg ttccccgtta ccgtcgacag ccgttccgtt
    38761 gtacgcatat accacaccat gggttacatc ggtgccggca aagtcatcct ggctattccc
    38821 aaggtcaaaa ttggtgtaca gcccgatgaa ggcgctgtcg taatccgaca gggaagaatt
    38881 tataatatca agatggatga aaagcgtatt tttcaaaacg gaatcattgg gtttatcgaa
    38941 ttcataagcc ataccatgaa tctcgacacc catcttatat ccaagtgttt ccgtgtgttc
    39001 ctgcattgaa tcgttcatta taaaaaacac agcttgatcg cccctgatca atgggtagtc
    39061 tcccgccatc ggattataaa cgctgtcgtg atcggcatcg tagaaaggtg caatttttgc
    39121 acgctgtcca agggaaacat ctccatgggc gggccacgag gcgatggcct caatgggggt
    39181 atagccggta ctgttccagt ttctgatatg atataatatc tctgaattgc taattttcca
    39241 tacacggttc catctcttga cgtaagaagt atcttttacc atcgaaaccg gtcccggaga
    39301 ataatcgttc ccgtccaacc ggaattgttc gcctgccagg cagagccggc cgttttgatc
    39361 ccatccaccg atccaaaggt tatggttaaa aatggccgtt tttccgcttc ctttcgggac
    39421 ttcaaaacgt gaagtatccc cgtcccagaa atggttcccg aaagggctga tcagggcatt
    39481 gatgttatta atgtctatct tttcgtaacg gggcggatac ttaagaatga ataccacgta
    39541 acctttagct gtcaggcctg atgcatcttt tattaaatat tcaacattac aggaagattc
    39601 atacatagtc ccgaaacagt actcactcat agtcagttca aaggtggtgt cattaatctg
    39661 cgtgagcgga ggcctcaggt aatagacata gatcggatcg ccgtccggat caaagtcatt
    39721 ttgaaggatg ttcagcgtgt aggtttgacc gataaaacca taaagagtgt cgtttacagc
    39781 aacaggacta ttattctgtc cgatcgaagc aacagaaaag gcaatagccc aaataatcaa
    39841 tagtaataat tttttcatct ttataaattt aatgttgaca caaatataca accggatcaa
    39901 tcatcattca acccgtcctc ccctgttttc ttaacaattt tttaacattt gcgcctgaaa
    39961 ttcttatata taggccatac aggctgaaaa atagtgcaaa atagcaatca aataaacatg
    40021 atagagtctc gcaaccctgg gtaacagaaa agcattacac agagccacgg agatttctca
    40081 ccgagaaata catgtttctc agtgaacctc aataacctca gtggctccgt ggttccctgt
    40141 aaaatcccgg tgtaaccctg tgttacaaaa aaaagaatta cacagagagc cacggagaat
    40201 ccacagaggt ttcacagaga aatacatgtt tctcagtgaa ccttagtgac ccttgtgcct
    40261 tagtggttcc cttcactttt ttccacagag caaaggtgtc acagaaacct gattcggatt
    40321 aataggatat tcccggctgc ttcgaacgcc tcttgaaaat ttcggaaacg gcatcaaatt
    40381 ctttcctgaa aataatacct accccttgtg tataaggcgc cctgcttgca ttcagatcga
    40441 gggaattgtt ggcccggtta taggctttca cccggaaacg gccgtcgtcg gtgattttta
    40501 cttccacatt cacgtcgcct acccatttat tgctggattg agaagtcgca gtcgcggtgg
    40561 tggcgccctg attcatcccc atgctcccgt cgatggtcac gcggtcatca aaaagttggg
    40621 tggaaagcgc cagttcaagt tgctgtgccg tcatctggtc gccggggcgg taattaacac
    40681 caatatcaaa atccttgctg atctgcgaaa gcatgttgct gatttggttg ctgatcatgt
    40741 caaacgacga tgatccaata tttgacgaga gtgtgctggt actctggttc aggctaaagc
    40801 tgttgagaac cagcagcgat atcatttgct ggttcatggc tacctgatta ctggtatcaa
    40861 tcacgctgta aataatacgc tgcgtttcgg gatcagcatc cggcatttcg atgctgaaac
    40921 ggatatccgg gttataaagg ctgttgctta gcgaaataat gcagttaacc gggacccttt
    40981 tgccggaagt gctttcggcc gatgctgaaa tcagcccgct taaatcgggt tttaccttat
    41041 atactgcgcg cagattgata tcggcatcgt cggggctgcc gttaaaggta atactgccgc
    41101 ctctttcaat ctgaaaggac cggctgaaga tattctggag cgaaaacagg aaattgcctt
    41161 catccatctt gtactgcccg tgcatggtat aattgccttt ggaatcgatg cccatattca
    41221 cgttgccgtc gccgcggact ttgatgttgc ccatctgata aggcagaatg atctcaagcg
    41281 ccgcatcggg agtggcctga atctgcaact gaaggtcgag gtttgaaaac tcacggggcg
    41341 gttgctcccg ggttgccgag tcccgggttt ttgtattcac ataccggata taatcgttat
    41401 ctgaaatatt taccgagtaa ttgataggca catgaatatc ggtgccttta tccgacctgg
    41461 ctttgatttt catcgaaagg tcattgaccg gccctgtgat ggagatgtct cctgttgcaa
    41521 tggctttgcc gtagaagtat tcagcagggt tgtaaggcgt attgagagcc gccattttat
    41581 ccgctttcag gtttatatcc agcctccagt cgctgaaggc gttgtgatag atattcccgg
    41641 tagcgagtcc cgaattgcca agcgaatcgt tgattacgat atccctgaac cacattttgt
    41701 ccttgccgaa attcaggtcg ccggcaaagg aataggaagt cttgatataa ttgaccagca
    41761 actgtgtgcg aaccaggtgc acagatccgg caagcacagg atcggaaaaa tcgccgatca
    41821 gcttcatttt gccggaaccg tatcctttca tcctcgagaa caaaccggta aagaaaggtt
    41881 caaacaaacg aactttgaga ttatcggctg taatatctat atcgaaatta tcatgcttcc
    41941 gttgaggaaa gacactgcca ttgaccagta acgggtaatg aagccctgcc gttccgtgat
    42001 aagcaatacg gccgtcaacg cttaaggcct tgtttacatc gtcccaggaa cttaacaatt
    42061 caaaatcgcc aagttgttcg tgattgaagc caaatccttt gaccgttaat tccgataccg
    42121 ctttaggcag gttgtagagt tccgaaactg aaacatcccc attgacaagc ccgtcgaaat
    42181 cgatgcccaa tttttttgtg agcacatccg aagctgaaag gtcgaaattc tcgaaagaaa
    42241 gggtcagcag atcaagggga tcgccggata tggccccgtt tattgaaagt ttctgccggt
    42301 tgcaatgcaa ctgcaggttt tggaaagaat agttattact gtcaataacc aacagattgt
    42361 cggagttcag cacccataaa ctgtcgttga taaccatcct ggcgccatca acggtggcca
    42421 ttaacctggg aaattcgttg aacgacacaa atcccttcag ggctcccttg ttacgatcgc
    42481 cagcatcgaa atcgttccat gcaaactgcc agttgacgct gtcattcact gcgtttgcag
    42541 tgagctgaac tttttcaata tgaaaacctg acgtatccgg aacatttccc ggagaagcat
    42601 ctattgagcc tgacaacaac gacattgtaa acttattggc ctgcgatacc ccgtttaacg
    42661 taaagtcgcg caaggacaac ccggatatcc tgatgagcgg agaatagccg tttatattga
    42721 tcagattgcc atgcggatcg aaactgcccg ataacaccgt atgcgtatca aactccaggg
    42781 aaggcaaaaa caaaccggta accggttttg tattctttaa ttttatcgta taatcgaatc
    42841 ttccctggtg tgcctgcggt ataatcgaca tgcccttcga aagcgacggc aggtaatcgg
    42901 taaagatcag cattatataa tcgcgcaggt ccttaaacgt gtattgtccg gtaaaagtag
    42961 catcagcgaa atcggagaca agggtcatct ttttgttatt gccttcgagc agcgatgttt
    43021 gaaactgcaa tctgtccatt ccaacaatgt ggccgctttc ataaaaaaca gtattatcga
    43081 aattcagaac gccgatcagg ttatccagag agttccccgt aaacctcagg ttcatggtcg
    43141 tggtaaggct ggtggaagta tcgcggtcaa taaggttcag ccgcgtcaga agggcgtcgg
    43201 tcatgtcggc acgaaaatcg aacgatggca ggctgtcgga aagatcaatc accccgtcga
    43261 aattcagccg ggccagttca tcatcaagtt tcactttccc cgagaatgct tcctgcctga
    43321 aggaaccgtc aatcctgatg ctgttcaggg tattgccttt cgctaccagg ttttgcacca
    43381 ggccgttgaa atcaagatcg gcggttttaa gcgacaggcc ttttccttta atgttagcac
    43441 tgagattcag tgttccgact tcagcggttt tcaatatctt gcccaggtcg aaattttcgg
    43501 cgctgacagt tccgccatat tcgatgatat gttttttctt gttgttggtc agtaaaatat
    43561 ccgtactgat ttttcccgca tccgaccaga atgcgccatt ggaaacaaaa tcattgtaaa
    43621 aaccggtaaa ccttcctttt atcgtaatca ttccaaaccg ataaaactct tgcggcaatt
    43681 ttatcctttg attttcgccc ggcaatccga aagcggcaat atcatcagcc gaagtagtaa
    43741 gattatcgat tttaaaatgg gcaaaagttt ccgtccagtc gggcaatcca ttcaatgtaa
    43801 cgtcccctct gtaaacggtg tttttcccgt atgtaatatt cattcctttg cccttaaaag
    43861 aggttacggt cccttttaaa tttccggcta ttccgaacac ctggttcatg cctttgattt
    43921 cgggcgcaaa aaacacgata tcgcgcatat caagttcaca cggccttaac cgggaattta
    43981 tgtacacgct atccaggaaa tcgttaaaag catgatacgc gggataatca aattcgaggt
    44041 caagatccag ccggctgtta tcggtaacca catgaagctg ttgcaccgaa aggtctcttg
    44101 atgaaaccgt ggccaggcct gaaaaatttc tgatgcagaa accacttctt tctatggcgg
    44161 aaagcgattt tatcatggcc gagatggtat cgccctttat tgagacattt cgcattttca
    44221 ggttcaggct tgatatgtcg atattgtcat aatccatgcc ttcagcctta ccagctttgc
    44281 tttgccggca atagttaaaa tgagaaccgg agattttcag ggttttgcaa cggatgatcc
    44341 atggctttgc tgttgtatcg ggaggtcccg gtttcgagaa ataatctatg atgaactgga
    44401 gattcaacac gctgtcgccg cggtactgaa caaggttaat atcggcttta tcaagcatga
    44461 tgcggctcag gttgatcacc cgctcgctct tgcttatctt tccgtatcca agtttgattt
    44521 tatcggcggc gataagcgtg tgatgatgcc ggtcgttgat cctgacatcc tttaccacta
    44581 cattcaaaaa ccaactgatg tcgactccgc caacgtaaac ttctgttttc agctcttttg
    44641 agagattgcc ggcaatttgc cgggtaatat atgtctgtac cgggcccgac cttaacaaga
    44701 tataggccgg tagcggcagc aggaaaacca ccagcaggat tcccatcaat atggatcggc
    44761 cttttttgat atttttgtac tttaaattgt aacgaagagg ttacagataa cttcaccccc
    44821 gatagtcttc aattattatg cccgtttata tccttgccat cgaatcatcg tgcgacgaca
    44881 cttcggcggc tgtatttgcc gatacgacca tgctttcgaa tgttgttgcc aaccagcagg
    44941 ttcataagga ttatggcggc gtggttcccg aactcgcctc gcgcgcccac cagcaaaata
    45001 tcatcccggt tattaacggg gctctcgtat cagcaaaaat agataaaaaa atgctgcatg
    45061 ccgtggcttt tacaagggga ccggggttat tgggctcctt actggtcggg acttccttcg
    45121 ccaaagcctt tgccctcggc ctcgatattc ctttaataga tgtgaatcac cttcaggccc
    45181 atgtgctggc ccacttcatt caggaaacag gaaaagaccg aaaaacgcct tccttcccgt
    45241 tcctttgcct tacggtatcg ggagggcata cacaacttgt cattgtacac gatcatttca
    45301 atatggaaac tgtcgggcaa accatcgacg atgccgccgg agaagccttt gacaaagcgg
    45361 caaagatact gggacttccc tatcccggcg gacccttcat cgataggaat gccgcgatgg
    45421 gccagcctgt tttcaggttt gccaaaccca ggatcccagg gttgaattac agcttcagcg
    45481 gattaaaaac atctttcctg tattatcttc gcgacaagct gaaaaccgag cctgcattca
    45541 tccaggccaa tatgaatgat ttatgcgctt cgatgcagca agccattatc gacatcctga
    45601 tggataaact gataaaagcc gcaaaacagt acaaaatccg cgaaatagcc attgccggcg
    45661 gtgtatcggc aaattccggc ttacgaaaag ctatccttgc acttaaccaa tcacacggtt
    45721 ataatgttta catccccgaa ttccaataca caaccgacaa cgctgctatg atagccgttg
    45781 ccggttattt caagtacctg aaaggcgatt ttgccgacca gtccttagcc ccctactcca
    45841 ggggcggaat ttagcaaccc gtgaccgtta tttgtacagg cagagatatg agacgtcacc
    45901 ttctgctttt accctgaact ttactccttt tgccacgata aatgtgctga acggctcata
    45961 ggatttccag gttgtttcac ccggcaataa aacgtgtaaa atccctgagg tcacggtcat
    46021 gtattcaatg gtggcggtcc cgaattcata ctcgccggct gccatcacac cgattgttgc
    46081 agggcattct gaagtattga aggcaaccga ttttacattt ccgtcgaagt attcgtttac
    46141 tttaaacata gtggtttaca tttattccgc tcaggcggtg gtgattatta attgctggat
    46201 ttcaggcaaa gagcaaagag caaggggcat ggagcaaggg gcatggagca tggagaacgg
    46261 agaacggaga acggggcaag gggaatggtg catggggccg ggtgcttttt acatatcatt
    46321 ttcttcggca agcgttttcc atgtttaaaa tcgggctgcc agtaacaatc cggttttatt
    46381 tgatggtttt tgactggatg ttaaaaaccg ttattctttt caagtgatca attcattgcc
    46441 agcccgctta tccgaaactc aattcaaccg accggcaaaa catgccgcct tatttcagca
    46501 cttcgaatct ttcattcact tccgtagaaa gtgttttaaa ggtcctgtca aagtggagaa
    46561 tattcttcct ggtcattccg ggaggcatat ttctgcgaat ataatcattc atcaggtaat
    46621 caatcattaa ttcagcgtgc tttcttcccg atgctgctgc catggcatag atatccctgt
    46681 cagtaatcct ggattccgaa tgttcaacga aatacatctt ttcatcaaaa ctatcgtatg
    46741 tgaatccgcc gtcgaaatcc tctttgataa aaccgctcag atacaacaaa cgtttttggt
    46801 ccggttttgc attctgatac tccacttccg tccaggtatt gaggccgaat gtcgacagtt
    46861 catactcctt gtaataatag ttttcgtcaa catcgtaggt atcatagtag tactttttgg
    46921 tttcttcctc catctgtaac tgcgcaaagc tgataatcca tttcggaccg ggttctgccc
    46981 ggaaagcgat gctgtcatca cacagcatta cccgatatcc cgccgatgtc attccgctga
    47041 tgaaggcatt ataaaatttt tcaagaaaaa gggaatcaga aacgtgttgc aggaatttgc
    47101 tcctgaaaaa gccggccgaa tccctggcct cttcacttat cagattgacg ggatcgggtt
    47161 cctggtatgt caggttcgtt ttataaatga aatcggacgg cacaaccagg agcgtttcat
    47221 tgtgctcttt cctgatatat tttcgtccca gccggtgttc aaccgtacag gatgaaaaca
    47281 cagacgataa aattaccgcg acagctattc cggaaagaat acggcaggat aggacagtat
    47341 gaggtttgaa gcgtaagacc attgatacaa gttaataaag cggaattccg gcaaccttta
    47401 atccgtcaca ctcaggccgg ctagcatctg ttctgcaaat ttgcggccat aatcaaccgc
    47461 tttcctgtaa tcctcttcgt gcggcgtgaa tttaacaaat acgccttccc cgaaaaattt
    47521 cagcttcagg tttgtaaggt tcgattcgat cattttcatc gattcgccgc tccacccata
    47581 cgagccgaag gcgccggcca gtttgccctt gtcgcggatc ggattaacgg ctgcaaacaa
    47641 ctggtaaaca ggcatcaaaa tattctggtt gatggtggga gaaccaacaa tcagcccggt
    47701 gcattccgac aggtaagcgt caattttatc gaggcccatc gtttcgatgt cgtgcagctc
    47761 aacatcaacc ccggcaacct gtttcgcccc ttcggctatt ttggcgccga tggtggctga
    47821 gttttgatag gcggaaacgt aggcgatgaa aaccatcggt ttggttccct gcccgagggt
    47881 ttcgcgggac agtttttccg acatgtcgac atatttcttc cagtgcgaac gaagtatcgg
    47941 cccgtgccct ggaagaattg cattgatttc aagcggcctg atcttttcga tggctttgag
    48001 cataaacctg ctgaaaggtt tcaggataac gtcgaaatag tatttgaacg aatcgtccca
    48061 gttgccggca aggtcgtcga acatagcttc atcgcaataa tgggcgccga acgaatcgca
    48121 ggtaaacaga agcttgtctt cctccaggta ggaataaaca gaatcgggcc aatgaaggtt
    48181 tggcgccccg ataatgcgta atttcttatt gccgagatca atgacatcct ggtctttcac
    48241 ctggatatgc gtgaaatcga atcctaacag gtcgcgcagg tagcggatcg cgttgccgct
    48301 gcctaccacc tttgcctttg gcgctatttc cagcaatttg ccgaggctgc cgctatgatc
    48361 gggttccgta tggtttacga tgatatattc aagctcggca ggatcgcaaa gtgtgcgcaa
    48421 cttttcaacg aaagtatcga aatacttcac tttcaccgtc tctacgatcg ttttacggtc
    48481 ggcattaatg aaataggcat tgtaggttgt gccgtaaggc gtgtgcatta ccacgtcaaa
    48541 tgttaccagt tcgcggtcga gcacgcctac ccatgatacg tcgtctgaaa ttttaagtac
    48601 tttgttgtct gccatggtga attaatttga tgtttttgat ttgaatcctg acagcttata
    48661 aacgcctgaa aggtttgaat tgtttataag aatctgttta aaatccttat caatattgag
    48721 tttgcaaaag atcattacaa taatcctttg attttcatga ggctttaaaa caacgacatt
    48781 tggataccgg gtccatcatc atcgtccctg ttgttttcat tctcctgttc aggccacggc
    48841 tttgtttcag tcgtgttttc ttcttcagtg cctgattcat ccccggcaag ctcttcttca
    48901 ttatcttctg tttcctctgt ttcctccgtt tcggcgacga ctgattcttc gaatggcaac
    48961 gggtcaagcc agtaagaatc tgccaccgga tgcgaggaca ggcgtttgcc ttttgccttc
    49021 atacccatca cttcgacaaa ggaatcaact tcgagcgatt cattttccag gggctttttc
    49081 acaccttcgt tgttataatc aacctgaagt acaggccggt aatcggtaga aatcaggtgc
    49141 aacgtcacac catcctcgtc gggtatgaag ggtacttttt tgtcggtcat ttccaccagg
    49201 aaccgtttca cgaagtaatg acccttttcg ggattgaaat aaatacaggt aacgggtttc
    49261 ttcgggttta atttttcgat aacgaccatg tcttcctcaa agtgattgga aaggtcgaag
    49321 ttcatcaggc ggtaacaccc tgacttcatg atcgttaaaa ttttatcatc ttccttgaaa
    49381 gcgcccaggt attttccccg ctgatcggtg ttgaggcgca tcacagtgtc gtcgtaccag
    49441 atgtcgcggg cgctcagcgt agacagtcct ttctcggcct ggacaatttt acggatggga
    49501 ttccgggaaa ggatgtttcc ctgcgatgcc cggcctttga ttgccagcga agcaaagtca
    49561 aagtcaaaat tcagtttttt aagtcttggc ttgggtttca gcgaaacgcg gataacctcc
    49621 gactcgccgt tcgggttggc cgtaaaatac agcaccgatg atcctttggt tcctttagtg
    49681 aggttatatt ccttgtcgcg gaccacgccg gtgaccgaga accgtttgac ataataagcc
    49741 cccatcctgc catcctggta tgccaggtta taaaccgtgc ggtcatcgtt tttcttgaat
    49801 acatcgatgt ggataacatc tttaccgaca aataattttt cagctaccct ggttacgata
    49861 aaagtgccgt cctgcctgaa aacaatgata tcatcgaggt cggaacaatc acagacatat
    49921 tcatcctttt ttaggccggt tccggcaaaa ccttcttcgc ggttcacgta gagtttttgt
    49981 gtggcggctg caactgcaac agcttcaatg gcttcaaaat ttctgatttc ggttttccgc
    50041 tcgcggttag cgccatattt ccgtttgatc tccatgtaat aggcgatggc atattcaatc
    50101 aggtgagcca ggttgtattt tactttctcc atctcctcgc acagcgcttt caatgtatcg
    50161 tcccattttt tcaggtcgaa cttggtgatg ctgctcatag gtttttcgat gagacgcttg
    50221 taatcttcct cttcgattgg cctgtaaaat tcctggtaat aaggttcaaa aagcgtgtca
    50281 agaactttga atacgatttc agccgaggtg gcattctcgt atttcggatt cttatacatt
    50341 ccctcacgga tgaaaatttt aagcaatgtg ttgaaaaaca ccttctctaa aagctcatcc
    50401 aggtgtatct gcaattcggc tttgagcaga ttaaccgtct gttgggtcga aatcctcagt
    50461 atctcgctta cgccgcagaa attcggtttc ccgttcatga taacgcagga gttgggcgat
    50521 tcgctccgct cgcagtcgct gaaagcatag agtgcatcta tggtctggtc gggcgaaacg
    50581 cctgactgca actggataag gatctcaacg ttccgggctg tattatcatc tattttctta
    50641 atcttgatct tgcctttgtc gttggcggcg atgatggagt caatcaggtc gccggtggtt
    50701 gtgccccatg ggatttcggt gatgaccagc gtttttttgt caagctggtt gatgcgggca
    50761 cggatgcgga ctttgccgcc acgcagaccg tcattgtatt ttgaaaaatc ggcaagccct
    50821 cctgtgggga aatccggtac aatgacaaac tcctcccctt tcagaatggc aaccgaagca
    50881 tcaatcagct cattaaaatt atgtggcaac aatttactgg caaggcctac ggcgatgcct
    50941 tccactccct gggcaagcag caacggaaac ttcacgggca atgagacagg ttctttattc
    51001 ctgccgtcat acgaagcctt ccactgtgtt gtttttggat tgaaagctac ctcaagggcg
    51061 aacttcgaca gcctggcttc gatgtaacgt ggcgcagcag cgctgtcgcc ggtaaggatg
    51121 tttccccagt ttccctgcat atcaatcagc aggtcctttt gtccaagctg gaccagggca
    51181 tccccgatag aagcatcacc atgcggatgg tacctcatgg tagtgccgat aatattggct
    51241 accttgttgt accttccgtc atccagttca tacatggcat ggagaatccg tcgctgcacc
    51301 ggtttcagcc cgtcaagcac ttcgggaact gcacgctcca ggatcacgta tgaggcataa
    51361 tcgagaaacc agttctggta cattcccgac aactgcatcg tacgatacaa ttctccggac
    51421 acagcttcgt cgtcttcagg cgtctccacg gccggttcat cctggtcaac aggcgattcg
    51481 ttatcttctt cttcaaaaat gtcttccttc tcttccatcg ggtaattatg aggatataaa
    51541 gttctgtaaa gtccgggaaa taatttcctt ccggtttatt tctttgctat cctcaacagg
    51601 atagcttcag ccgtcaggca catcagggcc aggatgacaa accatttcca caggcttatc
    51661 ccgttgttga tttcactaag caattgatcg acaggcttgt ttttagtttc aagaatgctc
    51721 accgacgggt ctttcaattt actgatcatc gcgcttattt cgtcacgggt gagacattga
    51781 aggtcgcttt catcccggtt ataattaaag gaaaggcttg ctattgccgc cggtccgttc
    51841 atcagaaagt aattgcctgc atttttaacc tgatcccgag catataggtt tattccgttc
    51901 ccagtgcggt gtatctcagg tatgatctca aaatcgccgg attctgattt cagcctgaag
    51961 actttgtcgg aggcggggga aggcacattg agccaaacag cttcatcttt accgataatt
    52021 gaatacagga aagccggcgg acggctcatc agagcaatat tatacagcac agggacaaag
    52081 agtgcctgct gttgaaaatt gctgaaaccg gcatccagtg gggctgcaaa ctggtacatc
    52141 tttcctttgc cggaattggt ttcaaccagg aaatcacggc cattgagcat tttcatgacc
    52201 ggaaccgtaa gggtaacagc tttggacgca atcggataat actgcgatat caaaggcaat
    52261 tcaaggttct gaggcagcct gcccggttcc tgatcaaata cgttccggaa tacgggatct
    52321 gtctcattca gcgtaattac ttttgaattg gaggtatcaa cggccatata aaccgggcat
    52381 ccggcctggg ccagcaggtt gttgtaagaa acgatatccg cacttttagc aggaaaaacg
    52441 gctaaagtgc ccccgttgtc aacataccgt cgcaattcct ggttaaggcc cgaggagatt
    52501 tccggcagtt cattcagaat caggagatta aaattttgca acgcactata gtccaatgat
    52561 ttctcattca cattctgcat ccgaacggtc gaatcctgcc cgaacagggc attcaggtaa
    52621 cggttttcgg ccccgccatt tatagtgagt accttgatgg atgaagttac ttcgaaagac
    52681 aggaagaatt tgtcgtcgta ggtaacagga taatccgtca cttcaacgac cgcctgctgg
    52741 aaaccaggct gataaatggt aaacggaatc ttaatttcac cggctccgcc ttcggacaaa
    52801 ctgattgttg ctacagcttt ctgcgttccg tttatactca gtttgaccgg gattttttcc
    52861 agcgatttat ccgatttgtt cctcacgcgg acattcagcg tcgaagtctg acctgtttgc
    52921 tgcagcggca attcgaacca acaactgtcg atataaacat tttcccgggt atttgctttt
    52981 aatggcagca aatacctttg aaaaccgctg gccgggatat cttcaaaggt tttaaacacc
    53041 gatttctgga aatcggaaat aataaaggct gttttttcag tttttccctc tgttttcaga
    53101 atatcatcct gacggctgac aatttccttc atcgtcctga cggaaggcga aggcttaacc
    53161 tcgttcagca tcaggaggaa ttcttcacgg gtaaccagtc gttgatgttt cccttcaaag
    53221 tcattggtga gcaactggaa cctgtccgat ggctgatagg ctgagactat ctctctcgct
    53281 ttccggcgtg cttcctccaa cagggttccg tcgggtccca tggcttccat gctaaaggaa
    53341 ttgtcgacaa aaatgctgac gacatttcca gatgcccgtg gcttcctgct ttctgaaaag
    53401 ggaatataag gctgggcgaa agccattacc agagaaacaa tggccagcat acgcaatgcc
    53461 agcaccacaa ggtgcctgag cctggatttc ttttgcgttt cctgtttcag ttcccttaga
    53521 aacctgacat tggtaaagta taccttacgg aatctccgga aattgaacaa gtgaatgatc
    53581 accggaatgg aaaccgcgag aagcccaaga agaaacaaag gatttacaaa ctgcattctg
    53641 cctcattaat atgcaacaac aggcaaaaat aacgatttac gttacaagga atgacgcctg
    53701 tgaataagtt ttaccatcgg attctcattt cctgataacg attattttcc ggttaacact
    53761 acaccttttc cggctgtgcc cctgctcacc agtaacttcc attgcatctg atgcgaactg
    53821 ttccggcctg aattaaagat cgtcgtcatt cgcccggtcc cgccatgcgc gtgaaaacgc
    53881 aaattccatt tcatcttgat ccggattctc ccctgctgtc gtaatggttt ctgcggtttt
    53941 acaattctga ccgcgcattt cgcggaacat gccggaattt tgaacaaaac aatcctgcat
    54001 tatcagcaat tcattgaatt cttctgagta atcaatcata ctgattagtt gaaaatcaaa
    54061 cagcgaaaat aatgttgtaa aggcatccag ccgttaagcg ttttttatcc tgatattaaa
    54121 ctttaattaa gccgtaaaaa tgtattgccg catagcgcct tatggttgtc gaatgatgtg
    54181 gaaaagcgga cgccgcccgt tcaggggcct tccgaaagct gtagcccggt ctcagttcaa
    54241 ggtatgcctt aactgaaatg atggtactat ctggaaatct tcaaggtaaa tgagaatgta
    54301 gagccgaccc cgggggcgct tcggatattg atggtttgat catgggcttc aatgatatgc
    54361 ttcacaattg ctaacccaag ccctgaccct ccgccaacgc gggagcggtg cttatcaacc
    54421 cggtaaaaac gttcgaacag gtgcggcagg tgcacatctt cgatgccgat tccattgtcc
    54481 gaaacttcga tcagcaggtt ttcgtccata ttgtaaaaac tgacttttgt acgcccttgc
    54541 tcacggccat atttaattga attgtcgatc aggttaacca ggacctgcct gattttatcc
    54601 ttatcggcag atacgtactg gggaggaata ttttcatttg ccaggatcag gttaatctgc
    54661 cgggcctgtg ctttagcttc aagaaattcg aatacttccc gcgtaagggc tacgatatcg
    54721 aagcgcgaca tttccggttt cgcctctccc gactcaagct gactgatgat ctccaggtcg
    54781 ctgacaatag tgatcatgcg ctcagtgttc ttttccactt tctgtaagta atcccggtta
    54841 atttccgggt catgaatccc cccttccagc aacgtaagcg taaagccctg gatattgaat
    54901 aagggtgtct tcagctcatg cgaaacattc gccagaaatt cgcggcgaaa ggtttcctgt
    54961 tgcatcaggg catccatttc tttccggtgg gccgaagccc aatcagcaac ttcttcattc
    55021 actttatcaa gaacgttgcc gcgggtaact ttaaacaaat cccttttttc ctcttttgaa
    55081 agcttcaggc tatgaatggt cttataaatc aacttgatct ttgcatatat aaaactttgc
    55141 aggaaatacc ggatcaggaa aaactgaagc gccagcagaa tggcgccgga tataaccagc
    55201 gaaacccatg ggatttttgt tccacccgct aaggctgata accagtaagc aaatcctgac
    55261 agcaacgtaa gcgaagcaga aagatataat gctaatatac cagggtttga tgacttcatt
    55321 acctgacgaa tttatatcct acccctttta atgtatatat gtggtcgctg ccggtttttt
    55381 ccctgatttt ccggatatga acatcaatgg tccgttcccc cacaacaaca tcatcatccc
    55441 aaactttctg aagtatctca tctcgcgtaa atacgcgttc cggccgtgaa gcaagcaggc
    55501 aaagcagttc aaactccttg cgggcaagct gaatttcttt cccttccctg attaccaggt
    55561 acttctcctt atcaatgagc aggtcgccgg cctcgaaaac cggtgatgtc tccctgacca
    55621 ccttgtgcct tctcagtaaa gaattgactt tgctcaccag tagcgccggt tttataggct
    55681 tggtaatgta atcgtcccca ccggcatcaa ggctgagtat ctgggtgaaa tcttcgcttc
    55741 tggcggtaaa gaaaacaatg atggcatcat ccagggattt aatccgtttg atctcacggc
    55801 aggtttctac cccgtccatt tcgggcatca tcacatccag caataccagg tgcggggtta
    55861 ctttcacagc caggtcgatg gcgtcgcgtc cattggtcgc cgtatacacg gtgtatcctt
    55921 ctttccgcag gttatagccg acaaagtcga gaatatcttc ttcgtcatcg accaaaagta
    55981 ttataaatga attgttttcc ataagcatgc aaatatgatc attttctcag acaaaaccat
    56041 tatggcttac gtcctttggc cagcttgatg tgtttgatga cgatagcgtc gtacaaaaag
    56101 atcagttctt cgccgatatt caggacgtgg tctcccgaac gttccagctt gccgattacg
    56161 gtataggcat tcagataatc attgcattgt tccggctttt ccctgatcag gttcccgatg
    56221 atttcaacag cttcggtatt gtaactgtca agctttttat cgctctggaa caatccgttg
    56281 accagtgatt tatcctgatt ggcgaaggcc tccatggcct cctgcatcat cgattgtacc
    56341 agatagaaca tttccggtat gtgggtcttc cggaatactt cttcctccca tggaaatgaa
    56401 ataccctccg caatttttgc aaaagtattg gtataatcgc cgatacgctc caggttgtaa
    56461 ttgatcttga gtaccgaaat cacgaaacgc atgtcgatgg ctaccggatt tttaagcatc
    56521 aggatatttt cacaatccat atcaatcttc aggtcgaagg catcgacttt tttctcgttc
    56581 agcctgactt cgtgggccag gcttttattt gcctcaagga tagctatccg tgatttttcc
    56641 atctggctga tcaccaggct ccacatctgc agaaggtcat tcttcagaaa ttgtattgct
    56701 gtttctaact ggctcattat taataattat ttatggtgga tcatccggct ttacaaagtt
    56761 atgtattctg ccctcatcag ccctcggctg ttccgattat ccgaaacgcc ccgtgatata
    56821 gctttgggtt cgttcgtcag cagggttaag gaacattttc ggggtcttat catgttctat
    56881 aagtttaccc aggtaaaaga atccggtata atcgctgatt cttccggctt gttgcaggtt
    56941 atgggtgaca ataatgatcg tatactgctt tttaagctcg aaaatcaggt cctcgacttt
    57001 ggccgtcgaa atgggatcaa gggccgaggc cggctcgtcc atcagcaata aatgaggttc
    57061 cacggccagg gccctggcaa tgcaaagacg ctgctgctgg cctcccgaca atgccatggc
    57121 cggttttttc aggttatctt tcacttcttc ccacaatgca gcctgaatca gtgatttctc
    57181 cacaatttcg tcaatcgtct tcctgttctc cactccattg accctcaatc cgtatgctac
    57241 attttcatag atggatttcg gaaaaggatt tggtttctgg aacaccattc ctaccttttt
    57301 acgtatttcg tcaaccctga catctttttc atatatattc cggccatcca cggttatcgt
    57361 tccttcaagc ctggtatttc cgatcagttc gttcatccgg ttaaaaagcc tcaagaatgt
    57421 ggatttacca cagcctgatg gtcctattaa cgcagtaacc gtatttggtt caaaggtcat
    57481 gctgatatct ttcaacgcat gaaaatcacc ataatagaaa tttacgtttt tagcttctgc
    57541 ctgaatcatg aattcctgtt tttcaattct ttatactgtt caattacatt tttacccttt
    57601 tgcccaacgc tcttctgaga aaattcgcca ataagttgac cagcagaacg attatgataa
    57661 ggaccaaagc tgtgccgtat gccattggcc tggtttcttc catattggtc ccgctggtag
    57721 ccagtacata caagtgataa ggaagcgcca tcacctggtc gtatatgctt tgcggcaaat
    57781 ggggaagaaa ataggctgcc acggtaaaaa ggataggcgc cgtttcgcct gacacccttc
    57841 cgatggacag aattaatccg gtgattatgt tggggaatgc caccggaagc accacacgac
    57901 ggatcgtttg caattttgtc gcccccaggg ccaggctgcc ggttcgcagt gaatcatcaa
    57961 ctgatttaag ggcttcttcg gttgtccgga taatcaccgg taacgttaaa agccctaacg
    58021 tcatggaacc ggcaatgatt gaatccccga aacccaggta gttgacaaac aaggccatgc
    58081 caaaaagtcc gaagacaatg gaaggcactc cggaaaggtt gttggtcata aaatcaatga
    58141 aacgccggaa ccagttttcg gcgaggtatt cgttcatgta aatgcctgaa agaactccca
    58201 ctggaaatgc aaaaaccatg cttccaagta tgaggcaacc tgttccgatt atcgccggaa
    58261 agataccgcc ttttgtcatc ccttcttcgg gcatgccggt caggaattcc caactgattg
    58321 ccgacgctcc gcgcacaaca ataaatccaa ggatcaggaa aagtattccc accaccacca
    58381 aactgattaa ggtaaagaca gaaaaagcaa tgcgctggct gagttgtttc ctgtttattt
    58441 tgttgaagcg attcatctgt caatggtaat tttagctatt tgcgttctcc ttttttgata
    58501 taggcaaccg tcatattcac ggcaatagtg aataaaaaga gaacgatgcc caacgcaaag
    58561 agcgccttaa aatgagcccc cccgtaaggg gcctccccta gttctgcagc gatggtagcc
    58621 ggcatggtcc tggccggaac aagaaatgaa tgcggtaccg ttgacgcgtt ccctgtaacc
    58681 ataagcacgg ccattgtttc accaattgcc cgtccgacgc ccaacacaac tgctgccgaa
    58741 atgcctgacc cggcataagg aatagtgacc cggcgcagcg tttgccaacg tgtggcgccc
    58801 aaagcaaggc tggcttcttt tagcgatcgc ggggttgtcc tgagcgcatc ttcggcaatg
    58861 gtcacaatgg taggcaaagc cattatcccg agtaccacac tgcctgcaag aaccgtttcg
    58921 cctacgtcaa ggtgaaatac ccgctggata aaaggtacaa tcacaataag cccgaagaaa
    58981 ccgaatacca cggatgggat gccggccaaa agttcaacga ggggtttcac cacattacgc
    59041 accctgatgt ctgccacttc ggccaggaaa agcgctgttg ccaggccgag cggcaaggca
    59101 atcagcaagg cgccgaaggt gaccatcaaa gtcccgataa tcataggcca gataccaaaa
    59161 ccggggatcg gttcgctggt aggataccat tgctttccga aaagaaaatc gccgacggag
    59221 atgtttctga catgaataat ccgccccggg aaatctttcg ggatatcttt attattgata
    59281 tgcatcagcg ccccgggatg agtggaaata aactcatcca gcttcccggg gaggtactca
    59341 aaggatgccc ccaattcctc ttcggagtag taattggtga tgtcattcgt tgtaaaaaga
    59401 ataatgctgt catttttccc gccaacccgc gaccagtttg taatatcctg gttaaaaatt
    59461 gctctcaact gatcggaact caggtctttt acaggatttg ctttgctaac ggcaatgaca
    59521 tagtttttat caagcggaga acgggtgaat attcccaaac cctcccggaa aagaaataca
    59581 acgataagga tcaccgtaag gctggttatt gtactgcttg aaaacaggat tccttccatt
    59641 aatttctcca gggttctccg gtattgcttc atcagtagat atttttaaag ggtatcagtg
    59701 gcttaaccat ttcctgcatc gtcaaaccga aaacattacc ggattggcac ataaccttcg
    59761 ttgagcacta ttttttgtcc ttccggcgaa agcacaaatg tgacaaaagg acttactctc
    59821 ttttcaatgg ttttcagata atagtagtaa agcgggcggg ttatcgggta tttccagctt
    59881 tttgccgctt caactgatgg ggcgcaataa gatttccctt tatcgaaaga cacctgaatg
    59941 gctttaacat ttttcccgag ataggccagg ccaacgtaac cgatcgcccc ttttgtctgg
    60001 cttacgcttt gtacaatagc gcctgtagca ggcatcagca atgccgactg gctgaaattc
    60061 cttttattaa ggacgtgttc tttgaagaac tcataggttc cggaactggt ctcgcgggaa
    60121 taggtaacga ttttcatatc atcgccccct acttctttcc agtttttgat cttgccggta
    60181 aaaatgccct caagctgttc gcgggtaagt ttcgatacct tattggaagg attcacgata
    60241 acggccaggg catcataagc tataaccgtc tctttgaacg ctttaccggc atcgttgagt
    60301 ttcatttttt catcgagttt taagcttctg gatgcctggg ctatatccgt agtaccggat
    60361 agcagcgccg aaatcccgac tccggatcct cctcctgtca ccatgatgga tgaacccgga
    60421 ttctttttca tatatacttc tgcttccttt tgggttaatg gaagaaccgt gtcactgcct
    60481 ttaatcttaa agctcacttt ttgagcaaac accggtattg ataaaactgc cagtgccatt
    60541 aaaatcatct ttttcatctt tctgaatttt ttttgacaaa ttacgtttcc ctgtattagg
    60601 tggtatttaa gccaatgtta agtaattgtt aacccgcgtc agaatttata ctgcacgcgg
    60661 aatgtgaaga cattatcttt caggtccttc gtaaaatcat tggttgaatt aacgccctcg
    60721 ataccggttt tttcattctt tacaatgtca taataaaggg taaatttcac ctgcgaattg
    60781 aaccggtaat tccatccgag tccgaaggtg cggtacttta tatcagccgc gcttaagccc
    60841 gtgctttccc catcgttgtt tttcgcccgg atctgattcc ccgctatttt aatattggga
    60901 tcataccaat catattttag cacaacctgg tgtttcgtct ggccaatatt ctgaatacag
    60961 taaatgtatc cgccattgaa ttcgcggaga tatgtatcat agtcgggagc tgaagctgaa
    61021 gctacactga cgttactgcc tttacttcca ggctgttttc cggcaatgta ttcagccctg
    61081 atactgctca tgcctatcgc cgaaaagagg cttaactgaa gatcggctcc aagataccgt
    61141 cggcgtgcaa acccaaattt attggccgaa gcggaatcaa ccaggaaacc tctggttatt
    61201 ccgtcagtca tggtaccggt agaatacaga aatttggtac cctgcagaac gctgccgtta
    61261 taataagaaa tcccgaaccc gtatttgaaa ttttctgcct tgttggcttt gctcatacca
    61321 agccggccaa tgaaatcttt tcgtgtatcg gattcggcat tgatgccatt tccggcaaag
    61381 agaccggctt ccagcttaat aaaatcgaag cgtgtgcctt tttttggctg caaagtgagt
    61441 tttgccccca ggtcgcgttc accgggaaat attgattgga aaagacgcga tcgttccgga
    61501 gtttcgcggg tacccgacga atagctgatt tcgtaaccga acggacggtc aaatatgccg
    61561 gccgtcagtg atgcaaattc ggcccagggt tcacgaacgg taatataggc atcctttacc
    61621 cctactccct tttcggttgc atccaactgg atgacgaaag ccgaaagttc gttctcatag
    61681 ctaaatttca ccctgcccct ccgaataccg aacctgttgt cggaatactt cggaaaattg
    61741 cccccggcaa acgaagaaat tccggcagta tcggctttct gccactgagc ctggatatag
    61801 cctgttatcc tgaggcggtt caacagcccc atatctgttt ttatattttc aattttagca
    61861 ttcagggtat cggctgcaat tgccgcatcg ttctcctggg catcgcctgc cgtcggaaag
    61921 aggtagagga aaaccgcaac caatcctgcg gttaagtttt ttcgtaatat aaatttcatg
    61981 gttttgagtg ttttgattcg ccggcaaaga aaacgcgggc aatttatgcc cgtgttaacg
    62041 acagattaag aaaatattaa caggcatccc ggcaggccgt cagcatcagt ctgccttatc
    62101 ttcttctgca ggccctgaaa aatattgctt caacacggca aatccggagt cggaaagttc
    62161 aatttcgtat acgcctttat catactgaag caggcaggaa tgtcgtttca ggttcagttt
    62221 cctgaccatg gacaggttga ccagatattt gcgcccgcaa cgacaaaaag tgtcagcagg
    62281 aagtttctct tcaatatcgc ccagggcttc gaatgcccta acctgttctc cgtttttaag
    62341 gtacaaaaca gcataagcag aatcggcagc acaatagaca agttcatcct tgcgcaccgg
    62401 cataattcca ttcagcaccc tgaatgtgat acgttctgac aaactctttt cggaggcgcg
    62461 ggtatgcggt tcctgaattc cggtttgaaa actctgcatg cgttccatag cgctgataaa
    62521 gtcatcctga actaccggtt taaggatata atcgactacg cctaattgaa ttgctttgcg
    62581 ggtgtattca aatttactgt aagccgtaac aatgatcacg ggcgtgtgga ttccttcctt
    62641 gcggagcatc tctaggaaac taaggccgtt ggtttcaggc atgtcgatat ccatgaacag
    62701 gacatcatat ttctcctggc ttatcatcaa acgtgcttcc tgcacccctg aagccaggtt
    62761 gtaggcggca tccggataaa ggcgcgtgag cataagggct atcataagcc ttccgtgttg
    62821 ttcatcatcg actattagtg ctttcatagg aaaagccttt cgttgaatgg tctgtttttt
    62881 caggttttgt ataactcaaa gcaacgatgc agggaatgga attaccagtt cgaaccgcga
    62941 accggaagcc tttccttccg gagtgtacaa atcatggaca acatagttga aattccatct
    63001 tccctgttcg tttaaaaggc ggagctgatc ggcaaccaac tgcagcccca tgccatgtcc
    63061 gccggaattg atattctttg ccgcctcccg gccaattccg ttgtcttcga ccgaaattat
    63121 cacatttgtt ccgtcccggt atgccctgac ccagatcatc cctttgttgt tcaatttctt
    63181 gatgccatgg cgtaaggcat tttcgcacca cgtgtgcaat accataccgg gaaccggcaa
    63241 agaacccgga attgcgggat caactttaat gatgaaatcg aatttttcgc caaaacgcaa
    63301 cttttcaagg agcaggtata attcgatata ggagatttct tcatgaaggg tgcgcatcag
    63361 gttatcgata ttcatcagcg tttgatcgac ataccggtta aaaatattca ggtatttgcc
    63421 tgcgtcttcc ttttgccccg aaagaatgag gaatgaaatg ctgttcagtg cgtttcctgt
    63481 gaagtgaggt atcgactggg cccttaacgc ctgcagcttc agttgcgaaa gtttcctgtt
    63541 ttcttcacgc ttggccgtga tatacttgtt aataatcagc accaacagag taacctggat
    63601 tatacttgcc aaccccagca agatattcaa tacacggttt ttataccatg gaatggtaat
    63661 cacgacgggt tgtgaccggt acacccgcga ccatgtctgc ttgtcggtgg aacaaattat
    63721 ttcaaaaata tacttgccgg atggcaggtt tgaaaatgta gccactcttt gatccgaagg
    63781 atcagaccaa tgattggaat agcctaccag gcggtgcctg aaaataagct gatcgggatt
    63841 actcagacct atcccgtcaa agctgaataa caaggtattg taactgggcg gtaaaccttt
    63901 aggggtttca accgaagcta tcgggaacca gttcaggttg tctttcgtta ccgttatatc
    63961 attttgaata atcggggaaa ccggagttga tattttaaat aattccgggg caaaacatat
    64021 cactctgtca taaccggtga gccacaggtg attatcatta tcggggaaaa agccgttttg
    64081 accgggatca agaagattat atccgttttt ccggttatat aaagaataaa aatgcggctc
    64141 tcccttgtag atttttttca ggtcaattac aaacagtcct ttggtggtag ccgcaaaaag
    64201 gagatcctgg tggaggtaca aacatgatac aattcccctg atttcttccg gaagaatctg
    64261 aacttccggg atacagtacc cgatatcgtt taccagatgg caaaatggcc tatgttgtat
    64321 tctgaacgcc ctgaacaact gcgccatttt tcgtatcctg ctatcattgc aaaattcaag
    64381 atataagcca ttggttgtcg ctttccagat atttcccaac ttatcggcag tcatgctcag
    64441 cacccctttg taagctgttt ttctgttttc cctgaccagt ttcccatcta tgagataccc
    64501 ctgtgcataa tcagtaccga catacatttt cccggcttta tcctgggcca ggcaagtgat
    64561 ggctccataa tcccacaggt acttttcctt aagtctccat gaacttttat caaaaataaa
    64621 aagcccggat ccgttcttgc tgtcaccgcc ggtatagagg ttattccctg attcattttc
    64681 aaaattgatt agcaagtact tgtatggcaa taaccttaat ttttgtttat ccctgatgaa
    64741 aattccgttt tcggtattca tataaagtct cccttcatga tcacgaaatg agccgatatg
    64801 attctgttcc ttgaaggtat ttaaatcgga cgcaacagcc ggcagtccgg aaacaacacc
    64861 cgattttttc agcttcccgt tccggtccag cacccataaa ccatgtccgt aacatccgaa
    64921 ccacattgtg ccgttttcat cctgcaggat tgacccgata tcagcaatcc cttcgtcgat
    64981 agtcagaatg acctcttcga acttcaggtt agaaaaatta tggattcctt cattattacc
    65041 aatccagata ttgccttccc ggtcgcacaa accggcatta aatgaggatc catgtatgcc
    65101 aatgtttttg attaatccgt cggtttcaat gagttttacc tgcttgtccg aactgtcggc
    65161 aaagatgatc cggttttcat tatcaagaat tattggttgg tgaagtaccg gatatattcc
    65221 tgatggcttt gttttaacgg gttcttccct gacgatattc cccgggaaga ttgtcagcct
    65281 gtaaaatttg tgatttgaag aagaataccc tgccagggct ccgagtttct gattgaaagc
    65341 aaagcggctg aagcgatgat tcaggaattc tgttctgaac tttccgtttt tatatacaaa
    65401 acagcttgat tctgtgaata agaaccattc atcatgcgtt ctgaatacat caaggattct
    65461 atccgcagcc agaaagggtg taatctcaat gctattgctg ttcatattaa atgcggcaaa
    65521 acgggtacgc agcaactcgt cccggacctg aaagaacgcc atattacttt tttctacgta
    65581 cagcagcaaa ggcgtctgga gattaaatgt aaatccgtcc ggcaagtcgg ccagtttttt
    65641 gaggaccagg ttgtcggaaa taccataaac cgattttttc gccaacgcca gaatcagatc
    65701 gccggtttca ataattttaa atatactgct ggcgtcgcct tcaaaggtat tattgaactg
    65761 ctgaccatta aaacggctga tacctccttt tgtacaaatc caaagatagc ctctgctgtc
    65821 ctgcatcagg ctgcggatct gcgattgaac caaaccttca cgcgtcgaat agctgacgga
    65881 agcgaaccgc cttgtcgcta tttgtgccgt agcgccggta aagagcgtta ccgatacaaa
    65941 tactaaaatg aatgctattc gaaacaaacc aatcatatgt ttatttatat caaaaatacg
    66001 tgattatttg agaaataaac aaggccccgt gaaagtcagc cggggccttt atctaaactt
    66061 aactccctgt cggaaaagtc cgaaggtaaa atgcgtttat atgaaaacag aagactagaa
    66121 taaattgctg ctaacaattt tcataaagac attttaaaat tacaacagga ttattcacaa
    66181 ttcaaccgga ttcagttcct aattcatgaa cggggtttga tggctaatga acaggcaact
    66241 ttttcttacg aaccggctaa aaaaaagagg ctgtcaattc cgacagcctc caagtcaatt
    66301 agtgtgatga tatattagag tactccaaga tcaagcattg cattggcaac cttcataaaa
    66361 cctgcaatat tggcgccttt aacatagtta acatacccgt cgggttgttt tccgttcttc
    66421 acacaggctt catgaatgtt gatcatgatc tgatgcagac gctgatcaac ttcggtagct
    66481 gcccaactga gtttcatgct gttctggctc atttcgaggc ctgaagtggc gacaccgcct
    66541 gcgttgacgg ctttaccggg agcataaaga atcttgtgct cgatcagttc ttcaatagct
    66601 tcgggtgtgc aacccatgtt cgaaatttca cctacaagct tacagccgtt ggcaatcaat
    66661 tgttttgcat cttcgccgtt cagttcgttc tgggtagcgc aagggagagc aatgtcgcat
    66721 ttaacttccc agggacgttt tccctgaacg aattgtgctt tcgggaattc gtaagtataa
    66781 ggtttaacga tatcctggtt cgaggcacgc agttcgagca tataattgat tttttctccg
    66841 ctgatgccgt cgggatcgta aacgtaacca tcaggacctg aaatagtaac gacttttgct
    66901 cctaattcag tggcctttag ggctgcgccc catgcaacat taccaaaacc ggaaatagca
    66961 actaccttgc ctttcagatc ctgacctttg gttgccagca tttccttaac aaaatagatt
    67021 ccgccaaaac cggtagcttc cggacgaaca aggctgccgc cccagttaat accttttccc
    67081 gtaaggacgc cggtatgttc gtgggtgagt ttcttgtaca tgccatacaa ataaccgatt
    67141 tcacgtccgc cgacacctat gtcacctgca ggaacatccg tttcggggcc aataagtttc
    67201 cagagttcaa gcatgaatga ctggcagaaa cgcatgacct cattgtttga ttttcctttg
    67261 ggattgaaat ccgatccgcc ttttgcacca cccatcggca gcgaggtcag actgttttta
    67321 aagatctgtt caaaacccag gaatttcaga attgaaaggt ttacactcgg gtgaaaacga
    67381 agtccgcctt tgtaaggacc aatggcattg ttgaactgaa ccctgtatcc cagatttaca
    67441 tttactttac cgttatcatc tacccaggga actttgaagg taagaatgcg gtctggttca
    67501 accagacgat caataatgcc tgcagcctca aactgagggt tctcgttgta aacttcttcg
    67561 attgattcga ggacttctct tactgcttgt aagtactcaa cttctcccgg atgttttttc
    67621 tccaggttcg acatgatttt ttcaacttcc atgatcaaaa tgatttaaga atgattgtgt
    67681 atattcagga attctttgtt attgaattaa cagtacaaag gtattctttt aagtaaatca
    67741 cttaacaact gcctgaaatt tataatctgt caaaataaga ccggtcttcc ttttaaacaa
    67801 gcctattccg ctgttttaac aaccttgccg aagcggtagg tgattgtatc tttaatggtt
    67861 ccatcctcat taaagatcaa ctcgtcgccg tgtttgaggt tatttttata atgaacaacc
    67921 ctgatttttt ttccattggg aaggaatccc gtgagaattc cgcttccttt ctcgtaattc
    67981 ccctctccca cctttacgc
//
LOCUS       DAOPYV010000003        62752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_328_length_62752_cov_14.282709, whole genome shotgun sequence.
ACCESSION   DAOPYV010000003 DAOPYV010000000
VERSION     DAOPYV010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 62752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62752
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_328_length_62752_cov_14.282709"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..874
                     /locus_tag="PKI35_00575"
     CDS             92..874
                     /locus_tag="PKI35_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42902.1"
                     /translation="MKTSFKRAVLKALEQDKRPAGEILTEADIQHLPQAVQKYIRISG
                     CIGKEKVRNFRAEFTGKIRSTSADEFMSLRSVQYNFTGSPTRLFYIAAKKKGIPAKGV
                     HLYIDRKAIMLIKILGLFKVVDAKGPEMDQGETVTLFNDMCFMAPATLADKNILWDET
                     DDKTVHAQFTNGELKITATLYFNEKGELINFVSYDRFETLDGKTYLNRPWLTPVTGYT
                     DIKGYHLPAGAKLIFKHPDEDLCYGEFYLSDIEYNCREYRFR"
     gene            1115..1822
                     /locus_tag="PKI35_00580"
     CDS             1115..1822
                     /locus_tag="PKI35_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX42903.1"
                     /translation="MKPVAENLSADFGVLELLQTANSVDGQIAELHRQLASSADVPAV
                     LVGYSWGAWLGFLFAARYPDLVRKLILVSAGAFESKYNKDLTSIRLNRLRPADREEAE
                     RLISIITTGNPDCEAFRRFGELMTIADSFDCLPGSDYPVDLNADIYESVWAEASRLRE
                     TNELIENAARINCPVVAIHGDYDPHPVEGVKNPLFGRLENFRMVVINKCGHTPWRERL
                     AKDTFFEILWQEIKEVL"
     assembly_gap    1890..1989
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2120..3508)
                     /locus_tag="PKI35_00585"
     CDS             complement(2120..3508)
                     /locus_tag="PKI35_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HNX42904.1"
                     /translation="MMSDAYHQFIEDSLTKAFDPVHRKTIALNMSRYEAAVIKGKEQF
                     ANLEYARQRAAHIKNKVIIDLPKFLIDFETNFQSRGGKVIWAVDASEAVREVLKILKK
                     HHAKHIVKSKSMVSEELDLNNQLEKNGIESLETDLGEFIVQTAGQKPYHILTPAMHMS
                     KEDVASLFNQKFDLPAGSTPEEIAAFVRQLLREKFFTADVGITGANFLIADTGAVCVT
                     ENEGNAMLSMSFPKVHIAIAGIEKLIPRLTDLDLFWPLLASHGTGQKISAYNSVITGP
                     RQEGEADGPDEMYVVLLDNNRTTLMGRKNQRRALACIRCGACLNVCPVYRNIGGHAYG
                     SVYSGPIGAVITPWLNGMREYKHLSYASSLCGACTEVCPVKINLHEQLLYNRNDSVQA
                     GTARRKENMAMKAWKTAMLHRNMLDSVNGKTKNKVMKHFAANQWGPRRELPKFAEKSF
                     AQLWAERREGKN"
     gene            complement(3525..3959)
                     /gene="rpiB"
                     /locus_tag="PKI35_00590"
     CDS             complement(3525..3959)
                     /gene="rpiB"
                     /locus_tag="PKI35_00590"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNX42905.1"
                     /translation="MATLPIASDHAGYELKEFLKTKLAEAGYSLRDFGAYSAESVDYP
                     DIAHSVCSLVDKGQYPCAVLICGTGNGMCMTANKYPNIRAGLCWSKDIAELIRRHNDA
                     NVICLPGRFIEQGEALKAVMAFLTTAFEGGRHERRISKIPLK"
     gene            4213..5163
                     /gene="nadA"
                     /locus_tag="PKI35_00595"
     CDS             4213..5163
                     /gene="nadA"
                     /locus_tag="PKI35_00595"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNX42906.1"
                     /translation="MTTTRGQLSDKELIVKIDELRKEKNAVILAHYYQTPDIQDIADY
                     IGDSLQLSQQAAATDADIIVFAGVHFMAETAKILSPSKKVLLPDTEAGCSLADSAPAA
                     EFEAFVREHPGHKVISYINCSAAVKTMSDVIVTSSNAMKIVESFPKDQPLIFAPDRNL
                     GAYINGKTGRNMVLWEGACEVHETITTERIIGLKRMHPDAKLIAHPECKAPVLLLADF
                     VGSTAAMLEYTRNTGAKKFIVATETGILHQMQKASPEKEFIVVPADETCSCNDCPYMK
                     LNTLEKLYLCLKNEKPEITLPADVIEKARKPILRMLELSK"
     gene            5172..6716
                     /locus_tag="PKI35_00600"
     CDS             5172..6716
                     /locus_tag="PKI35_00600"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HNX42907.1"
                     /translation="MVRCLNIPFCLLFTVLLMMLAPSVKAQEGQNVINADGYNVFYYP
                     NGIKSSEGIFRNGKPDGYWKTYYENGLLKSEGNRKNFELDSLWKFYDDSSRLILTINY
                     AGGKKNGLKTTYRPGETMTENYEDDIKQGLTTYYYPDGKVKLTIPYVNGLEQGFAREY
                     APDGTVITYMEYKKGFMISRERINRKDKDGLRQGRWKVFYPSGIVQLDGVYRNDKKNG
                     YFKEYDENGQLLSVKKFVNDTEEKEAPELASLSIKTDYYPDGKVKTVASYNGEVPQGV
                     RREYDETGKITAGYVFHKGSMVGEGIIDEEGIKDGPWKEYYDDGTLRSAGTYDKGNRI
                     GEWKFYYENGKIEEQGRYNKKGNPDGTWQWYYDDGTLRREESFIAGLEDGDYSEYDET
                     GKLMVKGNYVEGLEEGEWMYDFGDYKEQGPYKGGARNGFWKSWYPDGTLRFEGGFIDD
                     NYNGKIIWYWPDGKRKDEGNYVNGSREGDWITYNEDGTPFLVITYKNDVEKKYDGVLI
                     KPPFEE"
     gene            6842..7477
                     /locus_tag="PKI35_00605"
     CDS             6842..7477
                     /locus_tag="PKI35_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNX42908.1"
                     /translation="MKRILFLSLISFFGLTVFCQELQPVKLLPPQIDKGKSLMQSLQD
                     RHSSREFSDKELSLQELSNLLWAANGVNRPGEKKRTAPSAMNWQDIDIYVFLKEGVYL
                     YNAFEMTLQPVVKGDLRSLTGMQPFVAGAPVNLVLVSDTKKMSRGDENSKTANASAEA
                     AFVGENVYLFCSAFDLNCVLRGSVDREKISKTLNLRPEQLAIYGITIGFPK"
     gene            7637..7846
                     /locus_tag="PKI35_00610"
     CDS             7637..7846
                     /locus_tag="PKI35_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42909.1"
                     /translation="MEPSSPSTTQGQPQPPQKKKRGCCFGCTLILILLLAGILGGAGY
                     LIYKSLPKKSAVEKEYNSVPDYFHN"
     gene            7853..11455
                     /locus_tag="PKI35_00615"
     CDS             7853..11455
                     /locus_tag="PKI35_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNX42910.1"
                     /translation="MKKSILFVLVFFAAGLTLFAQEPAFRLHMQAGKSQLAGDGDDYT
                     TVVITARDGEGEVMTNINGTVLLRCSAGFLEESELKMTNGIAYVKYTAPIFGQPIKAA
                     QRMVYFMVKFVRKFLARFNGSSDMASNQKLAGNIALETIREGTNPLTLIPQKNGDNNV
                     YFVCEMNGVKGKTKIQIVKTTEGGNSSLMPGYYSGYDITGQAPFELMLESGGKGQMTQ
                     SGSEPMTILFTNEKSTEINTAMKKMMGGGEWMNAYMGASEKDMQYMEGYDVKKNGLPS
                     IYLPMPNNGIFIYMPPILLEYQGRPKTPAAGASAASDETKKQERVFVTLEQNELIGDG
                     RSRTRAVFHYEDINGAPVSGKNIQWQIPKEFRIISVQNITDGSGNASAVIQAPVLRAT
                     EEKRGENTGGLIDNYDLFRLSVNYTSSENKNETTQTTLSVYKTLEKNLYILKPGFEPS
                     PYKVLLPQLEFYNLQSAVSATVSEGLGAQKSKLPLNDAIVFLESDKFDREYFRRIYDT
                     YFRKDRDMFITMLEKPEGGYAAFTGADGKFKLIVRDFEGKKRLYSGAYERTMPLEPLE
                     ARLADLTGRRTGTLTEVLGLLSGGKNAAGTTDNSGTYSETMLNTLDYKTKVYSQIAQM
                     EKVLCAGAFNEALGTEEKLHILGMLMTNAKGNARFMNDTGKELISNAWSLFMMAGEAA
                     NEYFKVSEKLGKKIGFDKVSDSLTKIGMKLDLGFWSKLTGTDRKTGTRRIVLDFIRKN
                     VLSSDAPNKIKGSGAYYRAMGEAYKALSGFVFEQLTEGISDALSEANPVPDLMVEIMR
                     ARYYAGLRDQVDKLLAQPPARVHAVYEQLQPVLRDRSTEIRAYYQSIGSQRFNAEMYK
                     ADWDYFRESVVKGGVIAYDLLTPNWHNLKKHLEALETFNKVTDAAYTTTMLSLEIYRY
                     HYLWCDARAAFDCANQSIDQGALVTAFLPEPSPEINFLNKAYAAGPPEKKGVPGIENL
                     VGLDFSFNKGSFPVENINKAIAAVTAYHQWIMADSARRASFTGFEPQKAGAVYKAAYD
                     FENQLSDLLLAAMAYAEDKSASRLREYEKAASALEISAASLAENSAIANARMDQIPSK
                     ITIPLSDFDEKDSLQFWRNPLYQKLGAGVLGLALVTVMVLWIVRRRSPRVVPAASALP
                     QSKAPSPQRSAVASAPDNPASPPPPPASPRFCPQCGSAFKPGAKFCGKCGYKPM"
     gene            11475..12344
                     /locus_tag="PKI35_00620"
     CDS             11475..12344
                     /locus_tag="PKI35_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNX42911.1"
                     /translation="MSLCVSCGNPLKPGARFCGSCGASITGIGESTTHPETFARDNRF
                     TTMDFTMKNSQRRIVNLWLFLTAVFIFLIFLPSIIGIDGFDGGFALSFASGFMVIMGI
                     IIVLIYRSRARQLDRILAGEGRIAVWKYSPDEWERFTAIDFEEEIKTKKALFYLISAI
                     CVVVGIIMLLVVKDILILYISLAIIPVVAIPAYLAPRMRYNKLKNSEGKVLIAEKGVI
                     VGRMFHLWVKLGASLDQVILVTDDIPPILEFHYSMPTRNGRQQEVARVPVPSGKMDEA
                     ERIGKYFGETAGA"
     gene            complement(12416..13297)
                     /locus_tag="PKI35_00625"
     CDS             complement(12416..13297)
                     /locus_tag="PKI35_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HNX42912.1"
                     /translation="MPFCSNCGTALKEGAVFCHECGTRNTIQSAAPAAPQAPPPQQPV
                     YQAPPPPLPQQAPPPAPPQIQSGPVSFDVVEKETLKFLKAELSNSALRYESGGMYYMQ
                     GNLEMEANLPSAGGFLKSMVTKESAVKPVIRGTGTVWFEPTFGNFTTLELNGDEWILD
                     KGSYFASEMGIEISSFTNKSISGLFSGEGFFQTRVAGHGKVVVISNGPLETIELNNGK
                     LVVDGSFAVARQASVQLTVAKANKGIFSTMISGEGIVNTFTGTGKVMIAPAANRYVTI
                     INYLSGIHHRVMQIKNS"
     gene            complement(13351..14433)
                     /locus_tag="PKI35_00630"
     CDS             complement(13351..14433)
                     /locus_tag="PKI35_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNX42913.1"
                     /translation="MKFCPQCGTTLIPGDRFCQECGFDISLIQDQAEPVAEETKPQPE
                     PVQAPVAGQFCPQCGKPLIAGDRFCQECGFDTQQPVTAKVDTAQPSAPPPVVPPLPQP
                     APVRKKGKSLLWVLIILGIAALGAGGWYGYNKFLKPADNEAVAADTTTQEQPVVTIDT
                     TVTVTEEDTVIETEEPLVAPTETKQVVEKKKEKQPAKTQAKPSARATKPAQTATQQQT
                     TPASPKPVITITPAESGTTGKATAVFTVGAKDNPKNMHPKNPTKCTLNRETTITRIIT
                     DHYNGGKGDPTGGTITMKDKGGNVIGQWRATPAANKNGVPGAKWICNINVNVKPGTYF
                     IQDSNPSTWSKNALSVGFVEIIGIEK"
     gene            complement(14548..15324)
                     /locus_tag="PKI35_00635"
     CDS             complement(14548..15324)
                     /locus_tag="PKI35_00635"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HNX42914.1"
                     /translation="MKFCPNCGAELKEGAKFCASCGFQIQNQEQTPPPPVQQPQQTFE
                     TREAASVFTDAISGKTNLVQRAINILTKPKVEWPVINNEQPNTMKLIFGYALILALIP
                     AIASFISLGVIGTSVMGFTYRSVSGGIMQGLIQLLSGVIGVYVLAWVIDMLAPSFDST
                     RNFGKSLQLAVYSYTASWVAGILLIIPGVRWLSFLVGLYSIYLLVVGTPVMKNTPKEK
                     VAGYVVITFIVMIVLYFLLALILGAILGLFFVGSMRGMGF"
     gene            complement(15453..17669)
                     /gene="rnr"
                     /locus_tag="PKI35_00640"
     CDS             complement(15453..17669)
                     /gene="rnr"
                     /locus_tag="PKI35_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNX42915.1"
                     /translation="MQKKSKKTTANIERNAFTNQVFNLFAENPSHSFNFRQTASQLAV
                     TDKKEKDEIRTILHNLCIQGSLVEMNRGKYKLNPELIVAVSKENTVTGIVDMKQTGKA
                     YVITPDLPEDVFINAGNTHHALNGDKVRIHLFPKRANRKTEGEVVEIVERAKKQFVGI
                     VRVSKGFAFLEPDSANVPVDIYIPLAALKGAKNGEKAVAVITDWPKRSANPVGKIIHV
                     LGEPGNNNVEMNAILAEFDFPLAFPKAAEEEAKKIKNEVPADEIARRRDFRDVWTCTI
                     DPADAKDFDDALSLKKLPGGNWEVGVHIADVSYYVQPGSAIDREAYERGTSIYLVDRT
                     IPMLPEQLSNLVCSLRPDEEKLCYSAVFTMNPKGEVLNEWFGRTVIRSNRRYNYEEVQ
                     SIIEGADGDFKEELMTLHGLSSKLRDERFKKGSIAFKSQDVKFSLDENGKPLGVIIKE
                     QKEANWLIEDFMLLANRRVAASIGDRKGEGEKKTFVYRVHDEPNPDKLANFTELLRKL
                     GYKFQTNNRRNISTSFNRLFDDIIGKGEENMIETVAIRTMARAEYSTHNIGHYGLAFS
                     FYTHFTSPIRRYPDLMVHRLLDRYLRNKPSVEQQEYEDYCVHSSEMERKAMEAERASV
                     KYKQAEYLMDKIGQEFDGLISGVSKWGLFVELNESKCEGMISLRYLNDDFYYLDEENY
                     RIIGQRWGRVYRLGDVVRIRVKKIDLQKKQMDFELVNDGIKNQASGTASRKTKGQK"
     gene            complement(17720..19450)
                     /locus_tag="PKI35_00645"
     CDS             complement(17720..19450)
                     /locus_tag="PKI35_00645"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX42916.1"
                     /translation="MGRNSDMSNSLHLSVSELTIQFKHEEGVAKAVDGVSFEVTRGRT
                     LGIVGESGSGKSVSSLAVMRLLPPGTAKVTSGKILFHLNNGESTDLLSLSDKEMRAWR
                     GKRISMIFQEPMTSLNPSYRCGRQVMEIILEHEKVSKKQARQRALALFDEVLLPRPAE
                     ILNAYPHQLSGGQRQRVMIAMAIACNPEILIADEPTTALDVTVQRAILNLLKSLQQKY
                     GMSLVFISHDLGVIAEIADEVAVMYKGKVVEKGPIRQVFTRPSHPYTRGLINCRPAPG
                     TRPVKLPVIADFLENDNPVVSLVPVTERLENHRVLYAKNPLLSAANIIVRFPVVRNFF
                     GKATRFVTAVNDVSFEVYPGETLGLVGESGCGKTTLGRSVLRLVEPSEGTIRYNNYDV
                     RAFTVKEMHSLRKKMQIIFQDPYSSLNPRITAGFAIMEPMKVHKLYGTDEARKEKALE
                     LLKKVGLEEAHFYRYPHEFSGGQRQRICIARALAVKPEFVICDESVSALDVSVQAQVL
                     NLLNDLKKEFGLTYIFISHDLGVVRYMSDRILVMQNGKLQEAGEADEIYTQPKSEYTK
                     TLIDAIPKGL"
     gene            complement(19463..20167)
                     /locus_tag="PKI35_00650"
     CDS             complement(19463..20167)
                     /locus_tag="PKI35_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNX42917.1"
                     /translation="MPDLLSEYMMRRLGEIREVTGKPQELGPVITFSRETGCNASGVA
                     HDLAIRLNEHAKEHNLRGGWQFINREILEKSAEKLHMEDTSHIKKVLTDKERNMMDEV
                     VEALSGKRHMSDLKIKKTTQDVLKEFAERGNIILVGRGGVIACRHITRSMHIRIEAPL
                     EWRINEIMNRFGYDRKYAREFVLKSDKERERVIECVTGGKTSNTLFDIIVNGSRFTIG
                     QITEMVFQLALAKEII"
     gene            complement(20192..21556)
                     /locus_tag="PKI35_00655"
     CDS             complement(20192..21556)
                     /locus_tag="PKI35_00655"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNX42918.1"
                     /translation="MTDLSVNKDNLIQFIADEELSAFQEEIDQCHSMLVNKTGKGSDF
                     LGWVDLPDQIDDELLTSIENDAKQIRQKAEVFVVIGIGGSYLGARAVIEALSHPFHPM
                     IKDEKSTSPVIVYAGNSLSQTYHARLIDLLDQKDYALAVISKSGTTTEPAVAFRILKN
                     HMEKKYGREEAKSRIIAITDKSRGALKKLSDDEGYKTYEIPDDVGGRFSVLTPVGLLP
                     IDVAGFDIRKLVAGAVGMRKHCLESSSVDENYAAMYAAIRNTLYRQGKQVEILVNYEP
                     SLQYLTEWWKQLYGESEGKDGEGLFPAGVGFTTDLHSMGQYIQEGQRILFETVLSVGS
                     PSRGLTIPFDPDNLDGMNFIAGKSLHEVNQMAALGTALAHIDGDVPNFSIHIPRVDEY
                     SVGELLYFFEFACGLSGYLLGVNPFNQPGVEAYKKNMFALLNKPGYEKESDAIRRRLG
                     QAQY"
     gene            21869..23467
                     /locus_tag="PKI35_00660"
     CDS             21869..23467
                     /locus_tag="PKI35_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX42919.1"
                     /translation="MKKSAFILFIVIVSLALATLIGMQVYWIQNAVRLREGNFQRSVD
                     EAVNAAVLKLEYIELSRHITQGEGIIKQESDSSLFYYQKRLVPGTGNEEADKAGSISD
                     ANDTLVNGANLITIDPRRNEPAPPPKHIGNNEPSPRHSALGMQELLRPGPEWSQPSII
                     HRMFSQLVGPAGVREIENYLSVGLIDTLLTSELAVKGIPIDFEFGVYNTYRNKIVLEK
                     TGRYHDILLGRSFTFPVFGSAGVSPTNYLLVYFPDQSRFILMNLWGLLTISAGLLFII
                     VMAFAYSMFTIIRQRKLSELKNDFINNMTHEFKTPISTVSLACQALTDNDIPHTPELY
                     NNYINIISEENRRLGQMAEKILQTAILEQGKLNLRFEKINLHTLIHDVIKNIGIQVEI
                     RDGSIATELLADDPYLKADKVHLSNMMYNLLDNANKYSPRRPTILITTENAENGVILK
                     VKDNGIGISKANQKKIFEKLYRVPTGDVHNVKGFGLGLSYVKFVVEKHGGTISVDSEI
                     NKGSTFSIYLPGNLMNTNASDKVK"
     gene            23503..24210
                     /locus_tag="PKI35_00665"
     CDS             23503..24210
                     /locus_tag="PKI35_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX42920.1"
                     /translation="MENNKIKVLLAEDDRNLGNILRTYLEAKGYATRLAVNGQEAFDA
                     FSKEPFAFCIVDVMMPVKDGFSLAKDIRKIDTSVPILFLTAKSLEEDKLKGFQAGGDD
                     YLTKPFSMEELLARMEAILRRTSQSQDMQGGKGFFEIGRFVFDYNHQILVINGQEQKL
                     TSKEAELLKLLCDHANQVVDRSFALNRIWQNDSYFNARSMDVYIAKLRKYLKEDTSVE
                     LINVHGIGFKLVTNPGA"
     gene            complement(24227..24922)
                     /locus_tag="PKI35_00670"
     CDS             complement(24227..24922)
                     /locus_tag="PKI35_00670"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNX42921.1"
                     /translation="MDPVELERYTEPLRQKFEAASDIKNAEGAKAYLRNMFDFYGIPS
                     PRRMEILAALKKETGYPAPGETDDFVKYAWQQPQREWQYAAMEILAAVSRKGDFRFME
                     TAVFMITSKSWWDTVDYIASNIAGNLFRKNPGRIIPTVDEWLKTDNLWLWRTAVLFQL
                     KYGENTDKELLFGLCERLSREQDFFIRKAVGWALRQYSKTDAEAVAGFVGSHTLSALS
                     RKEALKIINKGNS"
     gene            complement(24948..27296)
                     /locus_tag="PKI35_00675"
     CDS             complement(24948..27296)
                     /locus_tag="PKI35_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNX42922.1"
                     /translation="MKSSVLFKLLLCLAIFPAGFSHARPRPVAVPRLYSDTIDVVHYG
                     IHLTEINSTLKTLKGYTEVQFTPVINGISALPLELISLTVDSVIMNGSAIPNYSHSGT
                     LLRIPLLQVMNTGDTATATVYYHGTPFTDPSNWGGVHFSGEYVFNLGVGFDSDPHNLG
                     KAWFPCIDDFHDRALYDVYIRVSNDKKAVGGGNLVDITDHGDNTSTWHWKTEHTLPSY
                     LISFTTGQYELKTDIYNGMSGPVPITWYARPADTSKISATFVHLHDILQNFETHFGPY
                     PFERVGYSSTAQGAMEHASNISYPYSGWNGNTSLEWWYGHELSHMWFGDKVTCASAED
                     MWINEGWARWCESLLLGGLYGESAYKDDMRSKLKNVLQSTHITDGGYYALYGIPSGLT
                     YGSTVYDKGGQVVHTLRNYLGDSLFFGGVKAFLNEYAYHDMSSENLRDFLGNYTGIDL
                     TDFFETWVFTPGFPHFSVDSFSVQPVSGHYDVTVYVRQKSKGTTLLANSNHLEVTFMN
                     DQWQQYSDTLIFSGLTGSKVFSVPFEPVAVMADLNQKISDATTDYAATIKTTGELEFP
                     DTYVKVITGAVPDSAFVRITHNWVEPDSLKTPLTGLRLSDSHYWKVEGIFPEGFKAKA
                     KFFYSKSNLDNNLLLSSADSLVLLYRPGPAYEWYGVPFTQIGGWNIGFLEAETLARGE
                     YTLAVWDAQYAGQTEKNQLRNLHIFPNPARNSCSFETTSPSPCRVTIYNSTGNRVKAF
                     MIKAGNNTTRWDTRNEAPGLYFVHLSGEGGTEAAVHKLLIVK"
     gene            27438..28868
                     /locus_tag="PKI35_00680"
     CDS             27438..28868
                     /locus_tag="PKI35_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX42923.1"
                     /translation="MKKIFLLLCTLLASATGFPQQVWTLEKCIEYALSNNIQVKQQIL
                     QIDAGKSSLLQSRLDLLPSLNAGAQHGYNFGQTVDRYTNDFATKSVQTDNFYIGSELT
                     LWDGFKKVNQVRQNQADLEAARYETDKFMDDISLNIATAYLQILYYQEVLRTAQNQAG
                     ISQQQTDRLVKMVDAGASAQGDLYNIQAQLASAKVSVVDARNNLDLAYLTLAQMLDLP
                     SAEGFEIEIPNLDIGNQPALVANPDQVYGYALETQPVIKSAELRVKSSEYGLMLARGA
                     QSPQLSMSASLATGYSGAATMLGDSIKKTEPIGFTMINGVPDEIVYTSYTSFKSKDKP
                     FGDQINDNINRSLSFTLSVPIFNGWATRSAISRARINVENSKYTLDLRKLELRKTIQQ
                     AYADARGALDKYNASLAGVEAAHESFRFAEQKFNVGMMNSVDFNNARKEYEQADSDML
                     RAKYEFIFKTTVLDFYMGKPITLNRK"
     gene            28923..30302
                     /locus_tag="PKI35_00685"
     CDS             28923..30302
                     /locus_tag="PKI35_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX42924.1"
                     /translation="MFKKKNLIRILIILGVLLIIGFAVAKKKGVIGKPDVTKVATEMV
                     KKRTITETVSASGKIAPEVEVKLSPDVSGEIIELLVKEGDQVKAGDYLAKINPELYIS
                     ANDRAIAGLNTAKANLANAKARFAQVKAQFVNAKASFERNSKLFEQKTISQAEFDNAK
                     SQYEVAEAEVSAADENVRASEFSVNSAQAGVKEARENLTKTTIYAPVGGTISKLNVEK
                     GERVAGASQFSSGTEILRIANLQNMEVNVSVNENDIVRVNLGDTALVEVDAYQNRKFK
                     GLVTEISTSANTTGVSADQVTNFDVKIRILPDSYKDLIDPSHPNKSPFRPGMSATVDI
                     QTESVSNVLAVPIQAVTTRIDSTKIKQENAEMKDKKSQQGEGDNKEETKKKNGEIQEI
                     VFVYKDGKARKVNVKTGIQDNSYITIIEGLKETDEVIVAPYRTVSKKLKDGDEVKKVD
                     IKDLYKSEK"
     gene            30394..31113
                     /gene="tsaB"
                     /locus_tag="PKI35_00690"
     CDS             30394..31113
                     /gene="tsaB"
                     /locus_tag="PKI35_00690"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNX42925.1"
                     /translation="MPLILGIETATEVCSVSLLQDGKMISYKESAGNNEHSRLLTPFI
                     DEVLTVAGIKPDKLDAVAVSMGPGSYTGLRIGVSAAKGLCYALDKPLIAVGTLQAMAA
                     QVMAKLKNEINDEMALLCPMIDARRMEVYHAVYNQDLNEIKPVEAAIIGTDFLAKETT
                     NHQLILFGNGAAKCRDILSFRNNIVFLDNMGASANSVALLAEKKFFEHEFVDTAYFEP
                     FYLKDFIAAKPRVKGLYSNNG"
     gene            complement(31965..32035)
                     /locus_tag="PKI35_00695"
     tRNA            complement(31965..32035)
                     /locus_tag="PKI35_00695"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32001..32003),aa:Cys,seq:gca)
     gene            complement(32154..32672)
                     /gene="folB"
                     /locus_tag="PKI35_00700"
     CDS             complement(32154..32672)
                     /gene="folB"
                     /locus_tag="PKI35_00700"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNX42926.1"
                     /translation="MDLRDFCFICGKITLPSLLSSLFVTNMFASQHLAIIIPAGFYIL
                     LPIYLHLRNFMGIISIKGMEFFAYHGCFREEQVIGTRFLVDLVIDTDTGQAEINDDLK
                     KTINYQSVYKIVRTEMQQKSHLLEHVARRILDSVYSNHPEIKHLTVIVTKLNPALAAG
                     GKVREVSITLER"
     gene            32850..34550
                     /locus_tag="PKI35_00705"
     CDS             32850..34550
                     /locus_tag="PKI35_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNX42927.1"
                     /translation="MTENNPDSLPEEKVPANFIHQIIQEDIKNNKNEGRVHTRFPPEP
                     NGYLHIGHAKSICLNFGTAKKFGGKCNLRFDDTNPVKEDTEYVDSILEDVRWLGFEWD
                     GLYYASDYFENLYEYAEKLINKELAYVCDLSQEEMSLQRGTVTKPGTDSPYRQRTVEE
                     NLRLFRAMRAGEFPDGSRVLRAKIDMAHPNMLMRDPILYRIIHAEHHNTGNKWCIYPM
                     YDFAHGQCDSIEGITHSICTLEFEVHRPLYDWFIEKLEIFAPRQIEFARLNLSYTVMS
                     KRKLLQLVNEGFVNGWDDPRMPTLCGLRRRGYTPESIRRFAEIVGVAKRDQVIDVSLL
                     EFCLREDLNKRADRLMAVLNPVKLIIDNFPEGKVEILPAGMNPEEPEKGFRQVPFEKE
                     LLIEKEDFMIDPPGKFFRLKPGGEVRLKYAYIIKCESFVQDPVTGEVSEIHCTYEPDT
                     RSGMPNSNKKVKGTLHWAAMNHSVKTEVRLYDRLFSKESPEEVEEGQDFKANLNPDSL
                     KVITACCEPAIEQYHQGDKIQFERIGYFCVDPDSEPGKIVLNRTATLKDNWAKISGKE
                     "
     gene            34604..34676
                     /locus_tag="PKI35_00710"
     tRNA            34604..34676
                     /locus_tag="PKI35_00710"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34637..34639,aa:Gln,seq:ctg)
     gene            complement(34747..36270)
                     /gene="gltX"
                     /locus_tag="PKI35_00715"
     CDS             complement(34747..36270)
                     /gene="gltX"
                     /locus_tag="PKI35_00715"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNX42928.1"
                     /translation="MREVRVRFAPSPTGPLHIGGVRTALYNYLFARKHGGKCILRIED
                     TDQGRFVPGAEQYIIESLTWCGIHFDEGVHTGGPYAPYRQSDRKDIYHQYALQLLNSG
                     KAYYAFDTPEELDKLRKDYESRKETFQYGPKTRNSLSNSLTLSNQEVETKLSAGKPYV
                     IRIKIPENETVTLQDIIRGTVQVDSTLLDDKVLYKSDGMPTYHLANVVDDYLMKISHV
                     IRGEEWLPSAPLHVLLYRYLGWEKQMPEFAHLPLLLKPDGNGKLSKRDGDRLGFPVFP
                     LRWTDPKSGEVSSGYRESGYLPEAFINMLAFLGWNPGTEQEIFSMDELCEAFTIDRVS
                     KSGAKFDLEKAHWYNHQYMSRKTPAELASFLRPLLIEKNINFDEDYVEKVCALVHERV
                     VLKPDLWTQSQYFFSDPENYDEKVVLKIWKPETPALLQDLIHLIKATEPYTEENLEAT
                     IHAYIAEKSIGMGKLMNPFRLAVVGTNAGPGMMDIAALIGKEKIIQRIQKAIDRIKV"
     gene            36395..39814
                     /locus_tag="PKI35_00720"
     CDS             36395..39814
                     /locus_tag="PKI35_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42929.1"
                     /translation="MNTLLIFWRKLSFSYMRLESKEMENNYYLLLRKIDAFIRKYYKN
                     QLIRGAIYSLALLALFFILISALEYFSWFSTTIRTILFYSYLTATAFILGRFVIIPLL
                     KLRRIGKIISHEEAANIIGRHFTDVKDILLNTLQLKNQCDDELASRDLIEASIDQKTE
                     KLRPVNFTGAIDLKQNRKYLKFALPPAILILAVLIISPSAVTEPATRILHHKSIFEKP
                     APFEITVLNSHLKAVQQEDFELKIKLTGNEIPGELFLRIGDAVYRMEKENNIRFNYTF
                     KNIQQRVVFNFEAGDYNSKEYTLEVLPKPIILSFDIQADYPQYTGRKPEKFENAGDIT
                     VPAGTLLTWKFYLKDSNVLYLRLFNKVRDLPTKNSNVITWSTRVNESAGYSVMVGNEH
                     FKNIDSLQYFINVIPDLYPEISVNEYSDSIYENRLYYKGSVKDDYGFSRLAFCYTVKS
                     EGSKATDNKVILKDISLEKNVLQQPFYFFMDVAALSLKPGDILEYYFEVWDNDAINHF
                     KSTRSQKGVFRIPTVDEIEKKTEEKNEDIKDRMETAISQSRKLQQQIENFDRKLIEKD
                     EIGWQEKQQLQQMIDKQKQLQNEIEEIKKENHDKALKEEQFKDIDQSIMEKQQKLEEL
                     FDQILPDEMKKMYEELQQLLEKVDKEKVQDMLEEMKMNNEDMEKQLDRNLEMFKQLEF
                     EKKLQETIDKIKESADKQEKLSEKTGDAKREELESISREQDVLNKQFDNIRNDLNDLE
                     KKNSELSDPNNLSNTDPMEESIQKEMDNSKEQLENKSGKNASKSQKSAGEKMQELGQK
                     LENMQNEMNEENNAEDIETLRQILENLVDISFGQEKLMTELNTIGVNNPKYLQVIQDQ
                     KNLKDDLKMVEDSLFSLSKRQSAVEPFVMREINAIDNNMEETLDALNGRTVNTAKSKQ
                     QYIMTSVNNIALMLAESMKEMQSNMSFGGSGKSKSQCNNPKAGNGKSGKMNSLRQLQE
                     QLNKQLGEMKNEMDKNGSKPGNKGSSQMSEKLARMAAQQEALRKQLQEIGEEMLKEGY
                     GDGKSIKQTLQNMEQTETDIVNKRITRETMLRQQEIVTRLLESEKAEQKRDLDEKRES
                     NEGKDRFYENPAAFFDYKKLKEKDNEMIRVVPPSLKPFYKRKANAYFLSFD"
     gene            39838..40284
                     /gene="ybeY"
                     /locus_tag="PKI35_00725"
     CDS             39838..40284
                     /gene="ybeY"
                     /locus_tag="PKI35_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNX42930.1"
                     /translation="MINFNFQNTALKLQQRQLLRSWIKEVISWEGLKTGEVNYIFCDD
                     ENLYELNINYLKHNTLTDIITFDYTERNKISGDIYISVERVSENAKKFDKKFYDELYR
                     VMIHGILHLAGYKDKDETSKIAMRRKEDECLNLLNRKFETNISDTK"
     gene            40321..42192
                     /gene="mnmG"
                     /locus_tag="PKI35_00730"
     CDS             40321..42192
                     /gene="mnmG"
                     /locus_tag="PKI35_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNX42931.1"
                     /translation="MFKEYDIIVVGAGHAGCEAAAAAANLGSSVLLVTMNMMTMAQMS
                     CNPAMGGIAKGQIVREIDALGGYSGIVTDKTMIQFRMLNRSKGPAMWSPRAQSDRMRF
                     SEEWRLSLEKIRNIDFWQDIVIGVTAEKGRVTGVRTSMGHEIKAKAVVLTNGTFLNGI
                     IHIGLKKFTGGRIGERAAHGLTENLHELGFESKRMKTGTPVRIDGRSINFDKMIEQKG
                     DDKPGKFSFSETQVLKRQRSCFITYTNTEVHEVLKTGFDQSPMYTGRIEGIGPRYCPS
                     IEDKINRFADKTRHQLFVEPEGWDTVEYYLNGFSSSLPEEIQVKAMKLIPGLEDARIF
                     RPGYAIEYDYFPPVQLMATLETKNIANLFFAGQINGTTGYEEAAAQGLMAGINAHLNC
                     KGKDPFILKRSDAYIGVLIDDLITKGVDEPYRMFTSRAEYRILLRQDNADLRLTEKGY
                     HLGLASQERYNRVREKISAVETLTNYLKNESISPDLINGFLEKAGTAPVKQKVKLSSL
                     LLRPQIGLQAMMEIIPDLNDFVTGKLHGDDEITEEAEILLKYEGYIEKETEIAQKLSK
                     FEDIALKPDFNYRSLTALSWEAREKLSKAKPATIGQASRISGVSPSDISVLLVYIGR"
     gene            42248..43156
                     /locus_tag="PKI35_00735"
     CDS             42248..43156
                     /locus_tag="PKI35_00735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX42932.1"
                     /translation="MTQLINNMETLQNCPVCGNESFHPCISLSDYFLTREDFKIVECD
                     KCSFRFTNPRPFSSELGKYYESKEYISHSNTRKGLFSILYQNVRKYTIRSKFRIISGL
                     TNGNSLLDIGCATGELLHYFQKKGWDVTGIEPNDNARKFAHEEYDLRVEDEDYLKKIN
                     TASIDVITMWHVLEHVSDLNGRLGEINRILNEDGILIIAVPNSGSPDAEYYGKFWAGY
                     DVPRHLYHFTKPTMHDLLRKHGFSCIKEFPMKFDAFYVSLLSEKYFCGKTRWIAAFFS
                     GLRSNLKARRENNYSSMIFVAKKSKI"
     gene            43289..47011
                     /locus_tag="PKI35_00740"
     CDS             43289..47011
                     /locus_tag="PKI35_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX42933.1"
                     /translation="MMKHDLFNRRSKILAIAFALSLSAALIITGFVRYQNQPAKIVER
                     LTRQVEHSAQMLNLEIAVLQTKLTEPEESVQSYLENHYSSYFEEKGQLFLIYQNNKLI
                     YWTDNAFPAPEIFDTNQFAAPVINSGNGWFLARSERRGNFRIIGLQLLKYNYKYHNDY
                     LPDYFFQKFQIPRNTELSPLNDSLHENLYKILSSDGKPLFCISFDGVPRPGPWAIPIL
                     FIFYTLSLVTLIALMFSLYRTVYSILRSNWLLPAFFLDVLIIRCLQFYFRFPAILYES
                     DLFSPVYYASSDILPSLGDLFINMVLILQISWYTYQGLNRSSRPVNLKKIPRNIILVT
                     GVILTGFLFHGMNLLIQSLVINSNIPFNLEGLFKLSLLSITGFTVVAGIMLAFLYFFY
                     SLSRWIYHAGNSPISFLIAGLLVSVFYAAYILVIGEFNPYYSLFLAVLFVTFYLQHRK
                     KPIGFIQLSQTVLFIFLLAILANYSLNVYNKHHELEKRKLLALHLTEYRDSLAEYKFS
                     KVSNEMVFDDTLSQQLRKVQDHPEAEQDVINYLTNTYFNGYWSKFRVQIVICYSGKKL
                     EIKPENTIVECCDYFNDIILKNGKQTSVSDLYFFDNALDMMYYTGRITLDPALTGLKM
                     PFFIYVEITSKINYFALGYPQLLLDKNISEPENLFDYSYAIYKKGELVKSAGKYSYDI
                     HELSERGSRGRSDNYNYKGYNHQVFTIDGNSNLIISHHKLSVSESIAPFSFIFIFLAV
                     FMLIIQMTDGRSLALKRRKISFMIRLQAALVGIILASSVVIGTVTILNLIKLNETKNM
                     DIVKEKMNSVRYELEILLSDYQHINFQDENLLSDALDRIANALFIDVNFFNADGGLIS
                     SSRRQIFDDKLISTQINPESQYNLLINHKSFYYHTETIGNYRFLSAYATVRNYDNKVI
                     GIINLPYFSRQKEQKQELLNFLATFANTYIILIAIAIFLALFISRYITKPLQLIRSKL
                     GNLQLGRQNAKIEWQGTDEIGDLVSEYNRMIDELGQKAELLAQSERESAWRQMARQVA
                     HEIKNPLTPMKLSIQHLMRAWNDKAPDWEQRLRKFSQMLVMQIDTLSAIATEFSDFAQ
                     MPEPEIQRIDLVPLLNHSVDLFRNFSAITIAFLPSVDHCYINADENQMLRVFNNLIKN
                     AVQAIPIGRNGNIRITLETSDRNCLIKVTDDGAGILPEQKDKIFSPNFTTKSAGMGLG
                     LAMVKNIVDMSGGRIWFDSIPDDGTTFLVELPLAGG"
     gene            47060..48568
                     /locus_tag="PKI35_00745"
     CDS             47060..48568
                     /locus_tag="PKI35_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNX42934.1"
                     /translation="MNPIKQLAGQTAIYGLSSIVARVINFFFVPLYTRIISTGSYGLF
                     SELMGYIALLQVVLTLGLETGFLRYANKEKDKSGLLFSTALVTLIAVSMLLLAVVWFA
                     APSLGKAMGHRPIYLIYVALILAFDAITAIFFAQLRFENRAWTFALFRSIKIISEVVF
                     NVLLFFVVPPFLARHSGSFLLNFIPARPDYGYILFAIMLSGVVAMVLFLPRLVRTSMK
                     FSASIWKRLMAYSLPLMVAGLPGVANDFIDRPMFRFFAPDTSPWQDQLGVYNAVIKLA
                     VFMVLFVQMFRYAAEPFFFASAEKKDIRKTYADVMKYFVAFCIFIFLGIVLYTDIFAL
                     ILGKDYRSGMGVLPIVLISNILLGIAFNLSMWYKLTDHTRYAIYITLAGLAVTFLLNV
                     FLMPVYGYYASAWGRFFSYIVMIVISYWLGAKYYPIPYDLRAIFIYFAVGLGIYGLST
                     LLNGQSLWIRMPVNTVLLLVYVAFVLKFERISVRRILSVIPIQKLKHKSKPC"
     gene            48562..48996
                     /gene="dut"
                     /locus_tag="PKI35_00750"
     CDS             48562..48996
                     /gene="dut"
                     /locus_tag="PKI35_00750"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNX42935.1"
                     /translation="MLIKIVNQSPYPLPEYATVHSAGLDLRAHISEEIRIEPLQRLLV
                     PTGLFIELPEGYEAQIRPRSGLAIKHGISIVNTPGTIDPDYRGEIKIILVNLSDAVFT
                     LQPGERIAQMVVARFEHITWNEVETLNETQRGAGGFGHTGTA"
     gene            49039..50787
                     /locus_tag="PKI35_00755"
     CDS             49039..50787
                     /locus_tag="PKI35_00755"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX42936.1"
                     /translation="MQKISILLLMFFLTVAVHPLQAQHRHRRDKKNNRTETSLAAIDR
                     GAVTAVFIDATKAKILGDLPKALALYQLCIEKDPNSDAAYYELAQLYYQQGDYSTAAR
                     MTEKASEIDPSNVWYKLLLNDVYNKTNNREGSLRIMQQLAKQYPGNIEYLYDLATSYL
                     MINKPSEAVETYDQIEGIIGISEEISMQKLRIYQIQNKPEKAEAEILRLVEAFPDNKV
                     RYYSVLAESFMAVGKEDKASEYYKIIAEADPDNPYIHISLADFYKRKGDTAQSFEELK
                     AGFANPGLDIDTKIRVLTTYYTIQDIYVNRKDEALQLAGILVRAHPAEPQAHSLYGEL
                     MLQDKQYLAARNEFRTLISIDSSKYAGWESLMQADASLQDWDALMNESSRALELFPLQ
                     PVPYLFKGIALLQLKQPGEATTILNSGEKLVSGNDALLSQFYSSLGDAYNQIKEYKLS
                     DENYEKALRIDANNSYVLNNYAYYLSLRGEQLDKAETMSRKGAELDPDNPANLDTYGW
                     VLYKLGKFEEAKTWIEKAVNLNTKDDPDLLEHLGDVNFKLGDTTKALQLWQKAKDAGG
                     NTPELERKLKDKKPDD"
     gene            50780..51619
                     /locus_tag="PKI35_00760"
     CDS             50780..51619
                     /locus_tag="PKI35_00760"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNX42937.1"
                     /translation="MINRNNFAGLLFIVVLMAVSCTPARKAIRTPLKEEGAEYLFQNL
                     KHHELKFDTFSAKFTATVIQNRKKTSFQGQLRIQRDSIIWVSVTPMLGIEMARFLLTN
                     DSIKYLNRINSTCLQKNFGYINEMLNRTIDFDMAQAFLTGNDFSFYENSRFKAQVDGQ
                     EYKLHTINRLKLKRMVRRSDPDISIPIQTIWLNPDNFKINKVVLNETDKDNRRFTALY
                     KKFETTDSQIVPSEIEYRVETNDDNLKIDILYSKIVINPDQLSFSFRIPENYTEVQEW
                     KQK"
     gene            51604..52965
                     /locus_tag="PKI35_00765"
     CDS             51604..52965
                     /locus_tag="PKI35_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNX42938.1"
                     /translation="METEVTRNNIAMLSFLHSKKFSFIAGVLMLILVGTDAMFGQKDK
                     TALQRRKVKIEREIRYTNQLLNETKKSKQSNLNQLYLINKKINRREALIQEIGTEVTG
                     IDNEVVRLNDTIDKLSVTLAGFKKEYAELIYNTYKNHNAYDRLMFIFASKDFNQAYRR
                     LKYMQQYTDYRKKQAAKIQETQLLLWNKKSQYEKQKSSKLLLIHNQQMEKQQLTIEQD
                     EKNLTIKSLTKQEKNLARKLQENQAALRKLQAAIEAVVAEEVRKAKAENARKAAAEAA
                     KSTVKTGTKTASKPAAGTKKITPPVSAPPIMSTTTEEISLSKDFAGNRGKLPWPVDRG
                     VIAVSYGEHAHPEFKNIKIRNNGIDIVTTAESRAKAVFPGVVSSVISIVNLNYVVIIR
                     HGDYLTVYSNLKSISVKKGDKVKIKQDLGSIFTDPEENKTMLHFELWHGTSVQNPESW
                     LSK"
     gene            53098..53274
                     /gene="tatA"
                     /locus_tag="PKI35_00770"
     CDS             53098..53274
                     /gene="tatA"
                     /locus_tag="PKI35_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNX42939.1"
                     /translation="MSKLGPWEIILIVAVILLLFGGRKIPEMMRGLGRGVKEFKDGMK
                     SDDDTSKPENGSGK"
     gene            53534..55435
                     /locus_tag="PKI35_00775"
     CDS             53534..55435
                     /locus_tag="PKI35_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNX42940.1"
                     /translation="MKYFAAVLLMLFLLPGYVSLAQQSQPVSDPAGSAATPVIVYDDP
                     MIAALDSLSNLKYFENNQKMRAQLAQNKYNFLPNFVPTYPDSVYYYRLQRLGVESPMG
                     LTFNPYVKNFIDLYANRRRGLMERVMGLSQIYFPMFEQKLNEYDLPLELKYLAIVESA
                     LRPDARSRVGASGLWQFMYGTGKVYGLQVSSYVDDRCDPVKSTVAACRHFRDLYSTYN
                     DWLLVLAAYNSGPGNVNKAIRRSGGKKTFWEIRQFLPAETRDYVPAFIAVNYVMNYAA
                     EHNLYPVIPRLDYNEIDTVAVHRPMDFTILSEKLKIPREELEFLNPGFLRGFIPATDI
                     NPYYIRLPRKTVADFVNNEQALYAFYQLKPYEPFQTTSNDESEYILKKHSHKVRKGET
                     INTVARKYSCSVGDIKRWNRLRSTRLKYGQRLAVYTRVKNPDYDPNLALKAKAAKNEK
                     QELMVSDSLSSALADADTLETDDEQIAGNAAPLQKPKAVTSSVKSETRQTAKPVYHIV
                     KKGEYLTKIAASHHVTIDQIKEWNNLKKSTLAVGQKLRVNAPGQPALAANNTDSVQKA
                     SPSKTSGTNKKAGGRNVIYTVQAGDTLWSIAQKYEGVTVDQIKEWNNISKGSSLKVGQ
                     KIKVILPGG"
     gene            55527..56549
                     /locus_tag="PKI35_00780"
     CDS             55527..56549
                     /locus_tag="PKI35_00780"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HNX42941.1"
                     /translation="MRKIIFPLCLSLFIGFISGCGSPRSGKSNATGKSSEILVVCEKS
                     QWDGPIGEAIRSVFGAEMEGLPESESRFAMVNIPYNSFTKFLQPHRNIFIVDINPEYG
                     KTKSTARQNIWSHPQRVVEIKAGSDSAFIRYFRKYGEAISLLYEQNERNCYHAAHSLD
                     RDVKAESIIEKETGLKMMIPAGFYLAKNKNGFYWLRKETTEMSLGIMIYTYPYTDTSQ
                     FSYSRIYAMRNKYTMDNIPGPTDGSYMLISDDVIKPVSRRIQFNKKFAVETRGLWYTK
                     GDFMGGPFINFTVLDEPRQRIVVLDGFVYRPNKEKRGYIRELESILWDTEFSGNLDVK
                     QAEKKK"
     gene            complement(56759..57769)
                     /locus_tag="PKI35_00785"
     CDS             complement(56759..57769)
                     /locus_tag="PKI35_00785"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNX42942.1"
                     /translation="MIFVTGGTGLVGSYLLFELTKKGERVRVLIRDKNGPSRIGKFFS
                     CLTPDKDHQMSLIEWVEGDILDISALDSALTGIDQVYHCAALVSFCKGDHARMMKVNS
                     EGTANIVNLCLKHGIRKLIHVSSVSALGQPENGNLVDENSVWKISGQNSGYAISKYSA
                     EREVWRGIEEGLNAAIVNPSVILGAVCHSVTANSLLRNMKRLMPFYSKGINGYVDVRD
                     VVASMILLMNSNISRQRFLINSETLSCRDFFSLGAEILNLKKPFIPVSSVALGFLWRI
                     SALLSFVTGTKPLFTRELARPMVSKTYYSAQKFTDLFGYSYIPVRQSLKDAFQILGML
                     EE"
     gene            complement(57817..59424)
                     /locus_tag="PKI35_00790"
     CDS             complement(57817..59424)
                     /locus_tag="PKI35_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX42943.1"
                     /translation="MTDNRRPNIYLPVAFALTLAAGLLIGYYLMRVSLVSDAFSFQRP
                     SKNSVEQIISYIKQNYVDTVNAEKLKEEAINGMLLSLDPHSQYIPASDFNEANDPLMG
                     KFDGIGVQFHLEYDTVIVMNTVTGGPSEKLGIRGGDRIVKVDGKNIAHVKITNEQVMK
                     LLKGPKGTKVKVTIKRKGVSSLIDFTIVRDEIPTWSIDISYPVSKNVGYIKLSKFSAT
                     THDELAEVLKKFKDQGIKKVILDLRGNSGGFLNEAIAVSDEFLPAKKLIVYTKGLNRP
                     KQSAYATSEGEWDDKPVVVLIDENSASASEIVAGAIQDNDRGTVIGRRSFGKGLVQEQ
                     MNFSDGSALRLTVARYYTPTGRCIQKSYSGGVEDYYMEYYHRFTDGELESADSVKLND
                     SLKYTTPKGKTVYGGGGIMPDVFIPLEKDSSLIFFNETANKGILYQFAFDYSDRHRAE
                     ITRYADWKKFDKAFKITPALYSEYVAYAGKNGVKASPRSLAASRYRISELLKAFIARN
                     IFNDAGFYPIFLRTDKTFLKAVEIVNR"
     gene            complement(59457..59951)
                     /locus_tag="PKI35_00795"
     CDS             complement(59457..59951)
                     /locus_tag="PKI35_00795"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNX42944.1"
                     /translation="MKDLCLLAFFLSTFFIATAQQPVKIYNPSADAKHEIALAVERAS
                     ASGKHVFLQVGGNWCPWCLRFHKLIAEDPQLDSLVKTNYEVVLVNFSRENENRDVLAG
                     LDYPQRFGFPVFVILDGNGKRIHTQDSALLEDNGKGYDRSKVINMFTMWSPDAIRKTA
                     EKYK"
     gene            60129..60977
                     /locus_tag="PKI35_00800"
     CDS             60129..60977
                     /locus_tag="PKI35_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNX42945.1"
                     /translation="MISIPVINAQTAGNVLRFGVVTDVHYAGIPDKGNLTYSQSLIKL
                     QSFVDTMNRLKPDFVIELGDFKDMDSLPIPAKTLVYLNRAEEVFSGFRGPRYHVAGNH
                     DEDCIPKGKFLSLVENTGISKDKSYYSFDVKGFHCIVLDACFDSSGAGYNRGHYKWHD
                     TNIPGEELSWLRSDLRETRLPALVFIHQRLDGSGNYFVKNASRVRRMLRHHHRVKAVF
                     QGHYHEGSFGCINGISYYTLKSLVEGNSKGGYNFALVEVDEAGNISISGFGEVKSETL
                     RCPGVL"
     gene            complement(61077..61457)
                     /locus_tag="PKI35_00805"
     CDS             complement(61077..61457)
                     /locus_tag="PKI35_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HNX42946.1"
                     /translation="MDITGKVSRILPLVTGQGRNGEWRKQEFVIDIDSGGFQKKVCFS
                     LWGDRIDQAALAENELVKVFFDLESREYNQRWYTEARAWKVEKQSASVAHAPVATQDA
                     PPSNISPFDEPSNSDNSEVNDLPF"
     gene            61608..62732
                     /gene="pdxB"
                     /locus_tag="PKI35_00810"
     CDS             61608..62732
                     /gene="pdxB"
                     /locus_tag="PKI35_00810"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: protein motif:HMM:NF001309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HNX42947.1"
                     /translation="MRIVADDKIPFLKGILEPFAGVTYIPGKDINREVAAGADALLIR
                     TRTRCDASLLAGTPVKFIGTATIGYDHIDFGFCENAGITWTNAPGCNASSVMQYMASA
                     LISVSKEKKLSLEGKTLGIIGAGHVGSQVETLAGLLGMKVLLNDPPRARREGCEKFTE
                     LNRLLERSDFVTMHVPLTMAGDEKTYHLMDTVMIGMMKPDAWLINTSRGEVVDSAALD
                     AALSMGEIAGAILDVWENEPKIDFELLRKVFIGTPHIAGYSADGKANGTSRVVRALCA
                     HFGLPLVSWYPENLPEPLNNASIRINDLLTEDAAILHAILHTYDIRNDCTRLRENPAD
                     FEKLRNYYPVRREFQAYTVTGSNLKPAVKDKLVKLGFKVS"
BASE COUNT        17828 a        14023 c        14325 g        16476 t
ORIGIN      
        1 ctgcacctta tccctgtaag gcttttgacc aaaaattttg gtttatcagg cggttactat
       61 tgcttaaaga ctttttaaac agaatcatat catgaaaacc tcttttaaaa gagccgttct
      121 taaagccctg gaacaagata aacggcctgc cggcgaaata ctaaccgaag ccgatattca
      181 acacctgccg caagcagtgc agaaatacat ccggatttcg ggttgtatcg gaaaggaaaa
      241 ggtaagaaac ttccgcgctg aattcaccgg taaaatcagg tcaacctccg ccgatgaatt
      301 tatgtccctc aggtcggtac agtacaattt taccggcagc cctacccggc ttttctatat
      361 tgcagccaaa aagaaaggaa ttccggctaa aggggttcat ctttacattg accggaaggc
      421 gatcatgctg attaaaattc ttggcctttt taaggttgtc gatgcaaaag gccccgagat
      481 ggaccaggga gaaacggtca cccttttcaa cgatatgtgc ttcatggctc ctgccacgct
      541 ggcggataaa aatattctct gggacgaaac ggatgataaa accgtgcacg cgcaattcac
      601 caacggtgaa ctcaaaatta ccgcgacgct gtatttcaac gaaaaaggcg aactcattaa
      661 ttttgtgagc tacgaccgtt tcgaaaccct tgacggaaag acttacctta accggccctg
      721 gctgaccccg gtgaccggct acactgatat caaaggttac catcttcccg ccggggcaaa
      781 actcattttc aagcacccgg acgaagacct ctgctacggg gaattttacc tctccgatat
      841 agagtacaat tgccgggaat accggttcag gtaaagaccg gattatctgc ctgtggcgat
      901 tccctgaaat ctgatataca tacaggagta tagaaaggca ttcccttaga aaaggccggc
      961 atgtgaaaca ggaagcccgt cttttgctat cttcacacat ttcatcgtaa catgcattcg
     1021 ataaaagttc agtaatctgg aaaccatcag aagatatggc caaccgcctt tccggacagc
     1081 ccttttacac gggggaccgg gagctgccgg cgaaatgaaa ccggttgccg aaaatttgtc
     1141 ggcggacttc ggagtactgg aacttcttca gactgcaaac tccgtcgatg ggcagatagc
     1201 agaactccac aggcaactgg cctcatcggc ggatgtgcct gccgttttgg ttggctattc
     1261 ctggggagcc tggctcggct ttctgtttgc agccagatat cccgatctgg taaggaaatt
     1321 aatcctggtc agcgcgggag ccttcgaaag caaatacaac aaagacctga cgagcatccg
     1381 cctgaacagg ctgagaccgg ctgaccggga agaagcggag cggctgatct caatcatcac
     1441 taccgggaat ccggattgtg aggcattccg gcgcttcggc gaattaatga ccattgccga
     1501 ttcctttgac tgtcttccgg gcagtgatta cccggttgat ttgaatgcgg atatctatga
     1561 atccgtttgg gcggaagcct cccggctgag ggaaaccaat gaactgattg agaatgcggc
     1621 aaggattaat tgtccggtgg ttgctatcca cggcgattac gatccgcatc ccgtcgaagg
     1681 cgttaagaac ccgttatttg gcaggctgga aaatttcagg atggtggtaa tcaataaatg
     1741 cggacatacg ccctggagag aaagacttgc caaagacacc ttctttgaga ttttatggca
     1801 ggaaattaaa gaagtgctgt aatccgaacc cccgcctgat gaaattaagg ccctcatgcc
     1861 acgaaaccgg tgctttcgtt ttgcggcctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnna atagaaccaa cctgataatc ctgaaaccct cacaggaatt ctctgaaaag
     2041 aagcctggat caactgttta acagggaatt gacaacattc ggtgcaacag gaaaaggaat
     2101 tctgacgccg aagaaccggt cagttttttc cttccctcct ctcggcccat aactgggcaa
     2161 aactcttttc ggcaaatttc ggcaattcac gccggggccc ccactggtta gcggcaaaat
     2221 gcttcatgac cttattcttg gttttcccgt ttaccgaatc gagcatattt cgatgcagca
     2281 tagcggtttt ccaggctttc atagccatat tttcctttcg ccgtgccgta ccggcctgca
     2341 ccgaatcgtt ccggttatag agcagttgtt cgtgcaggtt gatttttacc gggcatacct
     2401 cggtgcaggc gccgcataag gaggaagcat aactcaggtg cttgtattcc cgcatgccgt
     2461 ttagccaggg tgtgattacg gcgccgatag gaccgctgta gaccgaaccg taagcatgcc
     2521 cgccgatgtt gcggtatacg ggacatacat tgaggcaggc gccgcagcga atgcaggcga
     2581 gcgcccggcg ctggttttta cggcccatca gcgtcgtccg gttgttgtcg agcagcacca
     2641 catacatctc atccgggccg tcggcttcac cttcctgccg cggaccggta atgacggagt
     2701 tgtaggcgct gattttctgc ccggtgccat ggctggcgag cagcggccag aagaggtcaa
     2761 ggtcggtgag acggggaatc agcttttcga tgcccgcgat ggcaatgtgg actttcggaa
     2821 aagacatgct cagcatggcg ttgccttcgt tttcggtaac gcacacagca ccggtatcgg
     2881 cgatcaggaa attggcgccg gtaatcccca cgtcggcggt gaagaacttt tcccgcaaca
     2941 gttgtctgac aaaagcggca atttcttcag gcgtgctccc ggccggtaaa tcaaatttct
     3001 gattgaaaag cgaagcaaca tcttccttcg acatgtgcat ggccggcgtc aggatgtggt
     3061 atggcttttg tccggcagtt tgaacgataa attcgccaag gtcggtttcg agcgattcga
     3121 ttccgttttt ctccaactgg ttgttcagat ccagctcttc gctgaccatg cttttcgatt
     3181 tcactatatg tttggcatgg tgttttttca ggattttcag gacttcgcgg acagcctctg
     3241 acgcgtcgac agcccagatc actttgcctc cgcgcgactg gaaatttgtc tcaaaatcga
     3301 tcaggaattt cggcaggtcg atgatgactt tattcttgat atgggccgcc ctctgcctgg
     3361 catattcaag gtttgcaaac tgctcttttc ctttgatcac ggcagcttcg taccgtgaca
     3421 tattcaaagc gatcgtcttc ctgtgtaccg ggtcgaaagc ctttgtaagg gaatcttcga
     3481 taaactgatg atatgcatcg ctcatcatac aaaggatttg ctgattattt caatgggatc
     3541 ttgctgatac gcctttcatg gcgccctcct tcaaatgccg tagtcaaaaa tgccatgacc
     3601 gccttaaggg cttctccctg ttcgataaac cgcccgggca gacaaatgac atttgcatcg
     3661 ttgtgtcgcc ggatcagctc ggcgatatct ttgctccagc acaatccggc cctgatattc
     3721 ggatacttat tggctgtcat gcacatcccg ttgccggttc cgcagataag aacagcacag
     3781 gggtactggc ctttatctac cagtgagcag actgaatggg cgatatcagg ataatcgaca
     3841 ctttcggccg aataggcgcc gaaatcacgg agggagtatc ctgcttcggc aagttttgtc
     3901 ttcaggaatt ctttcagctc ataaccggca tgatcgctgg ctataggcaa ggtggccatt
     3961 ttatcaaaca taacgtatcg tttttagcta tgacctgttg atcaattatt tcaaaaaata
     4021 ctacggtatc aatcccggtt cagcaaatgt aaccatagat agggagttat tcattttcaa
     4081 acagctaaag tttttaaatg atatctgttc ataagttgac taattttgtg aaattgcaat
     4141 gccatagtaa tttcagccgc attgcctttt gtatcaagca acagttgaag cattaacaac
     4201 ggataatttg ccatgacgac caccagagga cagctatcgg ataaggaact gattgtgaag
     4261 atcgacgaac tgcgcaagga gaaaaacgcg gtgatcctgg cccattatta tcaaactccc
     4321 gatatccagg atattgccga ttatatcggc gacagtttgc agttatcgca acaggcggcc
     4381 gccaccgatg ccgatatcat cgtttttgcc ggcgttcatt ttatggccga aacagccaaa
     4441 atactcagtc cgtcgaaaaa agtgctcctc cccgataccg aagccggttg ctcgctggcc
     4501 gattcggcgc ctgccgctga attcgaagct ttcgtcaggg aacatcccgg ccataaggtt
     4561 atttcataca tcaactgttc agccgccgtt aaaacgatgt cggacgtgat cgttacttcg
     4621 agcaacgcca tgaaaattgt ggaatcgttt ccaaaagacc agccgctgat ctttgccccc
     4681 gaccggaacc tgggcgcata tatcaacggc aaaacgggac ggaatatggt gctgtgggaa
     4741 ggcgcctgcg aagtgcatga aaccatcact accgaacgga ttatcggtct gaagaggatg
     4801 catcccgatg caaaactcat tgcccatccc gaatgcaagg cgccggtgct cctgctggcc
     4861 gattttgtgg gatcgaccgc ggcaatgctt gaatatacgc gaaataccgg ggcgaagaag
     4921 ttcatcgtcg ctaccgaaac aggcatcctc caccagatgc agaaagcatc gccggagaag
     4981 gagttcatcg tagtgcccgc cgacgaaacc tgttcatgca acgactgccc ttacatgaaa
     5041 ctcaataccc tcgaaaaatt atatttgtgc ctgaaaaatg aaaagccgga gatcacgctg
     5101 ccggccgatg tcattgaaaa agcgcgcaaa cccattctgc ggatgctgga actttccaag
     5161 taataactcc tatggtcaga tgtttaaata taccgttttg cctgctattt acagttttac
     5221 tgatgatgct ggccccgtcc gtcaaggcgc aggaaggaca gaatgttatt aatgccgatg
     5281 gatacaacgt attttactac ccgaacggaa taaaatcgag cgagggaatt ttccgtaacg
     5341 gcaaacccga cggttactgg aaaacctatt atgaaaacgg cctgctgaaa tcggaaggga
     5401 accggaaaaa tttcgaactc gacagcctct ggaaatttta tgatgattcc tcccggctta
     5461 tcttaacgat caattatgcc gggggcaaga aaaacggtct gaaaaccact taccgtccgg
     5521 gtgaaacgat gacggagaat tatgaggatg acatcaaaca gggccttaca acctactact
     5581 atcccgatgg caaagtaaaa ctgaccattc cgtacgttaa tggcctcgaa cagggattcg
     5641 cccgtgaata cgcccctgat gggactgtca tcacttacat ggagtacaaa aaggggttca
     5701 tgataagccg tgaacgcatc aaccgtaagg ataaagacgg tttacggcag gggcgctgga
     5761 aggtatttta tccgtcggga attgtccaac tcgacggcgt ttaccgcaat gataagaaga
     5821 acggctattt caaggaatat gatgaaaacg ggcagttgct ttcggtgaag aaatttgtga
     5881 acgatacgga ggaaaaggaa gcgcccgaac tggccagcct gagcatcaaa accgattatt
     5941 accctgacgg aaaagttaaa accgtggcca gctacaacgg ggaggttccc cagggcgtcc
     6001 gccgcgaata cgatgaaaca ggaaaaatca ccgccggtta tgtgtttcat aaaggaagca
     6061 tggtggggga aggcatcatc gatgaagaag gcatcaagga cggtccatgg aaggaatatt
     6121 atgacgacgg caccttacgt tctgcaggaa catacgacaa agggaaccgc attggcgaat
     6181 ggaaatttta ttacgaaaac ggaaaaattg aagaacaggg ccggtacaac aagaaaggca
     6241 atcccgacgg aacctggcag tggtattacg atgacggcac cttgcgccgg gaggaatcct
     6301 tcatcgccgg gctggaagac ggcgactact ccgaatacga tgaaacggga aagctgatgg
     6361 tgaaaggaaa ttatgtggaa ggcctcgaag aaggggaatg gatgtacgat ttcggcgatt
     6421 ataaggaaca gggaccctat aaaggaggcg cccgcaacgg cttttggaaa tcatggtacc
     6481 ccgatggaac gttacggttc gaaggcggtt ttatcgacga caattataac gggaaaatca
     6541 tctggtactg gcccgacggc aagcgcaagg acgaaggcaa ttacgtgaac ggcagccgcg
     6601 aaggcgactg gatcacttac aacgaagacg gcacgccttt cctggtgatc acttataaaa
     6661 acgacgtgga gaagaaatac gacggtgtgt tgataaagcc gccgtttgag gaataaaaat
     6721 ccatcccatc cggaattgcg gcttttcatc ctttccgata tatcgctcct gaacctgaaa
     6781 aaatgaacaa tgaaaagttg atattcatct ctctgaaaca gaaacccaaa aatatcgacc
     6841 tatgaaacgt atcttatttc tttcccttat ttcattcttt ggcctgacag tgttttgcca
     6901 ggaactccag cctgttaagc tccttcctcc gcagatcgac aaaggcaaat cgctgatgca
     6961 gtcgctgcag gaccgtcatt cctcacgcga attctccgat aaggaattgt cgctgcagga
     7021 actttcaaac cttttatggg cagccaacgg cgtgaaccgt cccggagaaa agaaacgcac
     7081 ggcccccagc gccatgaact ggcaggatat tgatatttat gttttcctga aagaaggcgt
     7141 ttacctgtac aacgcgtttg aaatgacgtt gcagccggtc gtaaaaggag acctccgttc
     7201 gctgacggga atgcaaccgt ttgtcgccgg cgctcccgtc aacctggtgc tggtgtcgga
     7261 cacgaaaaaa atgagtcgcg gcgatgaaaa cagtaagaca gcaaacgctt cggccgaagc
     7321 ggctttcgtc ggcgagaacg tgtacctgtt ctgttcggct ttcgatttaa actgtgtgct
     7381 ccgcggatca gtcgacagag aaaaaatttc gaagacctta aacctgcggc cggaacaact
     7441 ggctatttac ggtataacca tcggcttccc taaatagctg tttatcttct gaatgaaact
     7501 tgaaaaaacg ggacaaattt tatcccgttt tttttgtatg tcaaaaattt ttctaccttt
     7561 tccgcaaaaa atattacagc cgatctttcg gaacgctcca tagcatcctg attactatcc
     7621 caataatctc catctcatgg aaccatcatc cccgtcgact actcaaggcc agccacagcc
     7681 cccccaaaaa aagaaacgcg gctgctgctt cggctgcact ttaatcctga tccttttgct
     7741 ggccggcatt ctgggcggcg caggctacct gatctacaaa agcctgccga aaaaatccgc
     7801 cgttgagaaa gagtataatt ccgttcctga ttactttcac aattaaccgg ccatgaaaaa
     7861 gagcatccta tttgtactcg tgttttttgc ggccggactt accctatttg cccaggaacc
     7921 ggcattcagg ctgcatatgc aggcaggcaa atcacagctt gcgggcgacg gcgacgatta
     7981 cacaacggtc gtcatcacgg cccgcgacgg cgaaggcgaa gtgatgacca acatcaacgg
     8041 taccgtgctg ctcaggtgca gcgccggctt cctcgaagaa tcggaactta aaatgaccaa
     8101 cggcatcgct tatgtcaaat acactgcccc catctttggc cagccgatca aggctgcaca
     8161 acggatggtc tatttcatgg taaaattcgt caggaaattc ctcgcccgtt tcaacggctc
     8221 ctccgatatg gcatcaaatc agaaactcgc cggaaatatt gccctcgaaa ccatccgtga
     8281 aggcaccaat cccctgacgc tcatcccaca aaagaacggc gacaacaatg tttatttcgt
     8341 ctgcgagatg aacggcgtaa aagggaaaac gaaaatacag atcgttaaaa ccaccgaagg
     8401 aggcaacagt tcactgatgc ccggatatta ttcgggatac gatattaccg gccaggcgcc
     8461 atttgagctc atgctcgaaa gcggcggcaa aggccagatg acacagagcg gatcggaacc
     8521 gatgaccata ttgtttacca atgagaaatc aacggaaatc aatacggcca tgaaaaaaat
     8581 gatgggcggc ggcgaatgga tgaacgctta catgggagct tcggaaaaag acatgcaata
     8641 tatggaaggt tatgatgtca agaaaaacgg gctgccctcc atttacctac ccatgcccaa
     8701 taacggcatt ttcatttata tgccccccat tttgctggaa taccagggcc gccctaaaac
     8761 tcctgctgcc ggtgcgtctg ctgccagcga tgaaacgaaa aaacaggaaa gggttttcgt
     8821 caccctcgag cagaatgaac tgatcggcga cgggcgcagc cggaccagag ccgtttttca
     8881 ttatgaagac atcaacgggg cgcccgtctc aggtaagaat attcaatggc aaatcccgaa
     8941 agaattcagg ataatttcgg tccagaatat aaccgacgga tcagggaatg cctccgccgt
     9001 catacaggcg ccggtgctga gggcaaccga agaaaagcgg ggcgaaaata cgggcgggtt
     9061 gatcgacaat tatgacttgt tccggctttc agttaattac acatcgtcag aaaataaaaa
     9121 cgaaaccacg caaacaacgc tctcggtata caaaaccctt gaaaaaaacc tgtatatcct
     9181 gaaaccgggc ttcgaaccaa gtccctataa agtgttattg ccgcaactcg aattttacaa
     9241 tctgcaaagc gccgtttcgg ccacggtatc cgaagggctt ggcgcacaaa aatcaaaatt
     9301 gccgttgaat gatgccatcg tatttcttga atcggataaa ttcgacaggg aatatttccg
     9361 ccggatttat gatacttatt tccgtaagga cagggacatg tttatcacga tgctcgaaaa
     9421 acctgaaggt ggatatgcag cctttaccgg cgccgatggc aagtttaagc tgatagttcg
     9481 cgattttgaa gggaaaaagc gcctttattc gggcgcctac gaaagaacca tgccccttga
     9541 accgttggaa gccaggctgg ccgacctcac cggacggcgt accgggactc ttaccgaagt
     9601 tttagggcta ctttcgggag gtaaaaatgc tgccggcacc accgacaatt ccggtaccta
     9661 ctcggaaaca atgctgaata cgctggatta taaaacaaag gtttacagtc agatagccca
     9721 gatggaaaaa gtcctgtgtg ccggcgcttt caacgaggcc cttggaaccg aagaaaaact
     9781 tcatatcctc ggcatgctca tgaccaatgc caaaggaaat gcccgcttta tgaacgatac
     9841 cggcaaggaa ctgatctcta acgcctggtc gttgtttatg atggctggcg aagccgccaa
     9901 tgagtatttt aaagtttcgg aaaaacttgg taaaaagatc gggtttgata aagtcagcga
     9961 ttcactgaca aagatcggga tgaagctgga cctgggcttc tggagcaagc tgacaggcac
    10021 cgaccgcaaa acaggaaccc ggcggattgt cctggatttt atcaggaaaa atgtcctgag
    10081 ttccgacgct ccgaataaaa tcaaaggttc cggagcctat tacagggcaa tgggtgaagc
    10141 ttataaagct ttatcgggat ttgtatttga acagctcacc gaaggcatct ccgatgccct
    10201 ttcagaagcc aatccggtac ctgatctgat ggtggaaatt atgagggcgc gctattatgc
    10261 cgggttaagg gatcaggttg acaaactgct ggctcaaccg cctgcaaggg tacatgccgt
    10321 ttatgaacaa ctgcagccgg tgcttcgcga ccgatctact gaaatcaggg cttactacca
    10381 atcgatagga tcgcaaaggt tcaacgccga gatgtacaaa gccgactggg attacttccg
    10441 cgaatcggtt gtaaaaggcg gcgtgattgc atacgacctt cttactccta actggcacaa
    10501 tcttaagaaa catctcgaag cgcttgaaac attcaacaag gttactgatg cggcttatac
    10561 cacaaccatg ctcagcctcg aaatatatag gtatcattac ctgtggtgtg atgccagggc
    10621 tgctttcgac tgtgcaaatc agagtatcga ccagggggcg ctggttaccg ctttcctgcc
    10681 ggaaccttca cctgaaataa actttttaaa caaagcgtat gctgccgggc ctcctgaaaa
    10741 gaagggtgtt ccgggtattg aaaatctggt cgggcttgac ttttcattca acaaaggatc
    10801 ctttcccgta gaaaacatca ataaggcaat tgctgccgtg acggcatacc atcaatggat
    10861 tatggccgac agcgcccgtc gggccagttt tacgggtttt gaacctcaaa aagccggggc
    10921 cgtttacaaa gctgcttatg attttgaaaa ccaactttca gatttactcc tggcagccat
    10981 ggcatatgcc gaagacaagt ctgcgtcacg cctcagggaa tatgaaaagg cggcctctgc
    11041 actggaaatc agcgctgcat ccctggcaga aaacagtgcc attgccaatg cccgtatgga
    11101 tcagattcct tcaaaaatca caattccctt gtcagatttc gatgaaaagg attctttgca
    11161 attctggaga aacccgcttt atcaaaaatt aggcgccgga gtcctgggat tggcgcttgt
    11221 aaccgtaatg gttttgtgga ttgtccgccg ccgatcaccc cgggtagtgc ctgccgcttc
    11281 ggcgcttcct cagtccaagg caccctcgcc ccaaagatcc gctgttgctt ccgcgcccga
    11341 taatccggct tctcccccgc ctccccctgc ctcgcccagg ttctgtcctc aatgcggatc
    11401 tgccttcaaa ccaggggcta aattctgcgg aaaatgcgga tataaaccaa tgtaaacctg
    11461 ataaccccct ttctatgagc ctttgcgtaa gttgcggaaa cccgctaaaa cccggcgccc
    11521 gtttctgcgg aagctgcggc gcttccataa ccggtatcgg ggaaagcaca acacatcctg
    11581 aaaccttcgc tcgggacaac cgttttacta ccatggattt caccatgaaa aactcacaac
    11641 ggcgcattgt aaacctgtgg ctgttcctga cggcagtttt tatttttctg atctttttgc
    11701 cctccattat cggcatcgat ggttttgacg gaggtttcgc cctgagtttt gcttcgggtt
    11761 tcatggtgat catgggcatc atcatagtcc tgatataccg ttcgagggcc cggcagctcg
    11821 acaggatcct ggccggcgaa ggccgcattg ccgtttggaa atattcgccc gatgaatggg
    11881 aaaggttcac cgccatcgat tttgaagagg agataaaaac caagaaagcg cttttttatc
    11941 ttatctcggc catttgtgtg gtggtgggta ttattatgct gctggtcgtt aaagatatac
    12001 tgatccttta tatttctctg gccatcatcc ccgtggtggc catcccggcg tacctggcgc
    12061 cacgtatgcg ttacaacaaa ctgaaaaatt cggaaggcaa ggtattgatc gccgaaaaag
    12121 gcgttattgt gggccggatg ttccacttgt gggtgaaact gggcgccagc ctcgaccagg
    12181 ttatcctggt taccgatgac attcctccca tcctcgaatt ccattactcc atgccgacgc
    12241 gcaacggcag gcagcaagaa gtggccaggg ttccggtacc ttccggcaaa atggatgaag
    12301 cggaaagaat cgggaagtat ttcggggaga cagcaggtgc ataaattata gcagcaaaaa
    12361 gaaagcgcag cagaatctca cgatttgctg cgcttttttt atcgatcatt tcaaactatg
    12421 aattctttat ctgcatcacc cggtgatgga tgcccgaaag ataattgatt atggtcacat
    12481 accggttggc ggccggggcg atcatcacct tgccggtgcc tgtgaaagtg ttcacgatgc
    12541 cttcgccgga aatcatagtg ctgaaaatgc ctttgttggc cttggcaacg gtaagttgta
    12601 cacttgcctg ccgggcaacg gcaaaggaac cgtcaacgac cagtttcccg ttattcagct
    12661 caatcgtttc gagcggcccg tttgaaataa cgacgacttt cccgtggccg gccacgcgcg
    12721 tttgaaagaa tccttcaccc gagaataacc ccgaaatgga tttgttcgtg aaggagctga
    12781 tttctattcc catctcggag gcaaaatacg aacctttgtc caggatccat tcatcgccat
    12841 tgagttccag cgtggtaaaa ttgccaaacg taggttcgaa ccaaacggtt ccggtaccgc
    12901 ggattaccgg tttaacggcg gattccttgg ttaccattga tttcaggaaa ccgcctgccg
    12961 aaggcagatt ggcttccatt tccaggttcc cctgcatgta atacattcct cccgactcat
    13021 accgtaacgc gctgttcgac aattcggcct tcaggaattt cagtgtttcc ttttcaacaa
    13081 cgtcaaaaga gacgggacct gactggattt gaggcggagc cggcggtgga gcttgctggg
    13141 gcagcggtgg cggcggcgcc tggtaaacgg gttgctgggg cggcggcgct tgcggcgcgg
    13201 ccggcgcggc actttgaatg gtattccggg tgccgcattc gtgacaaaaa acggctccct
    13261 ctttcagggc tgttccgcag ttactgcaaa aaggcatgtt tgtgagtttt taaggttatc
    13321 ctgattagtt ggttgcgata atttgaagtt ctatttttcg atgccgatta tttctacaaa
    13381 acctacgctc agcgcatttt tcgaccatgt tgagggattg ctgtcctgaa tgaaataggt
    13441 ccccggcttc acattgacgt taatattgca gatccatttt gcaccgggaa cgccgttttt
    13501 gttggcggcc ggggttgccc tccattgacc gatgacattt ccgcccttat ctttcatggt
    13561 aatggttccc ccggtcgggt cgcccttccc tccgttgtaa tgatcggtaa taatgcgggt
    13621 aatggttgtt tcacggttta acgtacactt tgtcgggttt ttcggatgca tatttttagg
    13681 attatccttg gccccgaccg tgaaaacggc ggttgccttg ccggtcgttc ccgattcggc
    13741 aggagtgatg gtgatcaccg gctttggaga cgccggcgtt gtttgctgct gagtggcggt
    13801 ctgggccggc ttggttgccc tggcggatgg tttggcctgg gttttggcag gttgtttctc
    13861 ctttttcttt tccacgacct gtttggtttc ggtcggcgca accagcggct cttcggtttc
    13921 gattaccgtg tcttcttcgg taacggttac tgtcgtatca atggtaacaa cgggttgttc
    13981 ctgagtggtg gtatcggcag caacagcttc attgtcagcc ggcttaagga atttgttata
    14041 tccataccag cctccggcgc ctaaagcagc tattccgaga atgatcagaa cccataacag
    14101 cgatttacct tttttcctga caggagcagg ctgcggcaac ggaggaacaa ccggtggagg
    14161 tgcggatggc tgcgcagtat caactttggc cgtaacaggt tgctgtgtat cgaaaccgca
    14221 ttcctgacaa aaacggtcgc cggcaatcag gggttttccg cattgaggac aaaactggcc
    14281 ggcaacagga gcctgaacgg gctcaggctg aggtttggtt tcctctgcaa ccggttcagc
    14341 ctgatcttgt atcagagata tatcaaaacc gcattcctgg cagaaacggt cgccgggaat
    14401 aagggttgtt ccgcattggg ggcaaaattt catggcgttg gctttttatt caattgtttg
    14461 tcaaaatacc gtacggttga tcgaacaaaa ggccctcaac cgtacggaaa ttatttcacc
    14521 agctatttaa ttgattttct gagactgtta aaatcccatg ccccgcatac ttccgacaaa
    14581 gaatagaccc agaatggcgc caagaatcag cgcaagcaaa aaataaagga caatcattac
    14641 gatgaaggta atcacaacgt aaccggcaac tttttccttc ggcgtatttt tcattaccgg
    14701 ggttccgact actaacagat agatggaata taatcctacc aggaatgaaa gccagcggac
    14761 tccgggaata atgaggagaa ttccggctac ccaggatgcc gtataggaat aaaccgccag
    14821 ttgcaatgat ttgccgaaat tcctggtaga atcaaacgac ggagcaagca tgtcaatcac
    14881 ccaggcaagc acataaacgc cgataactcc cgagagcaac tgaatgaggc cctgcatgat
    14941 accgccggaa acgctgcggt aggtaaatcc catcactgat gttccaatca cccccagcga
    15001 aataaaggag gcaattgccg gtatcagggc caggatcagc gcatatccaa aaatgagttt
    15061 catggtattg ggctgctcgt tgttgatcac tggccattca actttgggtt ttgtaaggat
    15121 attgattgcc cgctgaacca ggtttgtctt tccgctaatc gcatcggtga atacactggc
    15181 tgcttcgcgg gtttcgaaag tttgctgagg ttgctgtacc ggaggcggag gagtttgttc
    15241 ctggttttga atctggaatc cgcaggatgc gcagaatttt gcgccttctt tgagctcggc
    15301 gccgcaattt ggacagaatt tcataaaaga agtttttggg ttaaactgat ttgattcata
    15361 gcttaatacc ggtaaacgac ttccgacgtt tagaggtaac tcacaaatat acgttatgaa
    15421 atcaatcagg gcaagtgaaa aacaaagact gcttattttt gtccttttgt tttacgcgaa
    15481 gcagtgcctg aagcctgatt tttaattccg tcgttgacaa gctcaaaatc catttgcttc
    15541 ttctgaagat caattttttt aacccgtatc cgcacaacat cgcccagccg gtataccctg
    15601 ccccaacgct ggcctataat gcggtaattc tcttcatcaa gataataaaa atcatcgttg
    15661 aggtagcgta atgaaatcat cccttcacat ttgctttcgt taagctcaac aaaaagaccc
    15721 catttgctca ctcctgaaat caagccgtcg aactcctgcc ctattttatc catcaggtat
    15781 tcagcctgtt tgtatttgac gcttgcacgt tctgcttcca tggccttgcg ttccatttca
    15841 ctggaatgca cgcagtaatc ttcgtactcc tgctgttcaa cactgggttt attcctgaga
    15901 taacggtcca gcagccggtg caccatcaga tcgggataac ggcggatagg agaggtaaaa
    15961 tgcgtataaa aactaaatgc caacccgtaa tggccgatgt tatgggtgga atattcggcc
    16021 cgcgccatgg tacggatggc cacggtttca atcatgtttt cctcgccttt tccgatgata
    16081 tcgtcaaaca aacggttaaa ggaagtcgaa atgttacgcc ggttgttggt ttggaattta
    16141 tacccgagtt ttcgtaaaag ttccgtgaaa ttggccagtt tgtcgggatt gggttcatca
    16201 tggacccggt aaacaaaggt tttcttctcc ccttcccctt tccggtcgcc gatgctggct
    16261 gccacgcgcc tgttggcaag cagcatgaaa tcttcaataa gccagttggc ttctttttgt
    16321 tctttgatga tcactcccag gggtttcccg ttttcatcga ggctgaattt tacatcctgc
    16381 gatttgaagg cgatggagcc ttttttaaac ctttcgtcac gcaattttga cgacaatccg
    16441 tgcagggtca tcaattcctc tttgaaatcg ccgtcggccc cttcgatgat cgactgcact
    16501 tcctcatagt tgtaacggcg gtttgatctg atcacggttc gcccgaacca ctcattcagc
    16561 acttcccctt tcggattcat ggtaaagacc gccgaatagc aaagtttttc ttcgtcgggg
    16621 cgcagggaac aaaccaggtt tgagagttgt tcgggcaaca tgggaatggt acggtcgacg
    16681 aggtatatcg aagtgccgcg ttcataagct tccctgtcga tggccgatcc cggctgtaca
    16741 tagtaggata cgtcggcaat atgaacgccc acttcccagt ttcctcccgg cagctttttc
    16801 agcgaaagag catcgtcgaa atccttggcg tcggccgggt caatggtgca ggtccaaaca
    16861 tcgcgaaaat cgcggcggcg ggcaatctca tcggcaggaa cttcattctt tatctttttg
    16921 gcttcctctt cagcagcctt cgggaatgcc agcggaaaat caaattcggc caggatggcg
    16981 ttcatctcca cgttattgtt gccaggttca cccagcacat ggatgatttt tcccacagga
    17041 ttggcgctcc ttttaggcca gtcggtgatc acagccaccg ctttttctcc attcttggcg
    17101 cctttcagcg cagccagcgg aatatagata tccaccggaa catttgcgct gtcgggttcc
    17161 aggaaagcaa aaccttttga aacacgaaca atgccgacaa actgtttttt tgccctttca
    17221 acaatttcaa cgacctcgcc ctcggttttg cggtttgccc gcttcgggaa aaggtggatg
    17281 cgcaccttgt cgccgttcag ggcatgatgc gtgttgccgg catttataaa tacatcttca
    17341 ggaagatcgg gggtaatcac ataggctttg ccggtttgct tcatgtcaac gataccggta
    17401 acggtgttct ccttcgaaac ggccacgata agttccggat tgagtttgta cttgccccgg
    17461 ttcatctcta ccagcgaccc ctggatgcaa aggttatgaa ggatggttct aatctcgtcc
    17521 ttttcttttt tatcagtaac ggcaagctgg gaggccgttt gccggaaatt aaaactgtgc
    17581 gacgggttct cggcaaacag gttgaaaacc tggttggtaa aagcgttgcg ttctatattg
    17641 gctgttgttt ttttcgattt tttttgcata ggtttatttt tttattgctg tacgtcgacg
    17701 tcaaaaaaaa tgccaatgtt tataatcctt tcggaatggc gtcgatgagt gttttggtgt
    17761 attccgattt aggttgtgta tatatttcat cggcctcgcc ggcttcctgc agtttcccgt
    17821 tttgcatcac caggatccgg tcgctcatat accgcacaac cccaaggtcg tgcgaaatga
    17881 aaatgtaggt aagcccgaac tccttcttca ggtcgttgag caggttcagc acctgagcct
    17941 gaaccgacac atcgagcgcc gacaccgact cgtcgcagat aacaaattca ggttttaccg
    18001 ctaacgcacg ggcaatgcag atccgctgac gctgcccccc tgaaaattca tgcgggtaac
    18061 ggtaaaaatg cgcttcttca aggccgactt tcttcagaag ttcaagggcc ttttcctttc
    18121 gcgcttcatc cgtaccatac agtttatgca ctttcatggg ttccatgata gcaaaaccgg
    18181 cagttatacg aggattaagc gaagagtaag gatcctggaa gataatctgc attttcttgc
    18241 ggaggctgtg catctccttt acggtaaaag ccctgacatc ataattatta taacggatag
    18301 tcccctccga tggttcaacc agtcttaaca ccgatcggcc gagcgttgtt tttccgcacc
    18361 ccgattcgcc aaccaggccc agggtttcgc cgggatatac ctcgaaactg acatcgttga
    18421 ccgccgttac aaaacgggta gcctttccga aaaagtttct gaccaccgga aaccggacaa
    18481 taatatttgc ggctgaaagc agcggatttt ttgcatataa aaccctgtga ttttcaagcc
    18541 tctcggtcac aggtaccagg ctaaccacag gattatcatt ttccaggaaa tctgcaatca
    18601 ccggcagttt caccgggcgg gttccgggcg ccggacggca atttatcagg cccctggtat
    18661 agggatgaga cggccgggtg aagacctgcc ggatgggccc tttttcaaca accttgcctt
    18721 tatacatcac ggcaacttca tcggcaattt cggcaattac ccccaggtca tgtgagatga
    18781 agaccaggct catgccgtat ttctgctgaa gcgatttcaa taaatttaaa atggctcttt
    18841 gaaccgtaac gtcaagcgcc gtggtaggct cgtcggcaat caggatttcg gggttgcagg
    18901 caatggccat agcaatcatc acacgctggc gctgtccgcc cgaaagctgg tgcggataag
    18961 cattcaggat ttcggcaggg cgggggagga gtacctcatc aaaaagcgcc aatgcccgtt
    19021 ggcgcgcctg cttctttgaa accttttcgt gttcaagaat aatctccata acctgcctgc
    19081 cgcaacgata ggaaggattc agggaagtca tcggttcctg gaagatcatg gaaatacgct
    19141 ttccgcgcca ggcacgcatc tctttgtcgc tgagcgataa caggtctgtg ctttcaccgt
    19201 tattcagatg aaataaaatc tttcctgaag taaccttggc cgtaccgggc ggcaacagcc
    19261 gcatgacagc cagtgacgaa accgacttgc cggaacccga ttcccccact atgccaagcg
    19321 tcctgccgcg ggtaacttca aaagaaacac cgtcaactgc tttggcaacg ccttcttcgt
    19381 gtttgaactg aatggttaat tccgaaacgg ataaatgcaa tgaatttgac atatcagaat
    19441 tccggcccat ggcctgttac gctcagatta tttctttcgc cagcgccagt tgaaagacca
    19501 tttctgtgat ctgacctatg gtgaaacgac taccattaac aataatatcg aacagggtat
    19561 tgctggtttt tccaccggta acacattcga taacgcgttc acgttcttta tccgatttca
    19621 ggacaaattc ccgggcatat ttccggtcgt aaccgaaacg gttcatgatt tcgttgatgc
    19681 gccattcgag gggggcttcg atgcgtatat gcattgaccg cgtaatatgc cggcaggcaa
    19741 tgaccccgcc ccgccctacc aggatgatat ttccccgttc ggcaaactcc ttcaggacat
    19801 cctgtgtggt tttttttatc ttcagatcac tcatgtgtcg ttttccggaa agggcttcaa
    19861 ctacctcatc catcatattt cgctccttgt cggtaagaac tttcttgata tgagacgtat
    19921 cttccatgtg gagcttttcg gccgattttt caagaatctc acggtttata aactgccatc
    19981 ctcctcgcag gttgtgctct ttggcatgtt cattcagcct gatcgccaga tcatgggcaa
    20041 cacctgaggc gttgcagccg gtttcgcgcg agaatgttat gaccggcccc aattcctgtg
    20101 gcttaccggt aacctcacga atttcgccga gacggcgcat catgtattca gataaaaggt
    20161 cgggcataaa acctccttct ttaaacaatt ctcagtattg cgcctggccc agccggcgac
    20221 gtatggcatc cgactctttc tcgtacccgg gtttattgag cagggcgaac atattctttt
    20281 tgtaagcttc tacaccaggc tgattgaaag gattgacgcc aagcaggtat ccgctgagcc
    20341 cgcaggcaaa ttcaaagaag taaagcagct ctccgacaga atattcgtca acccgcggaa
    20401 tatgaatgct gaagttggga acatcaccgt cgatatgggc cagggccgtt ccgagggctg
    20461 ccatctggtt cacttcatga agtgatttac ctgcaataaa attcattcca tcaaggttgt
    20521 cggggtcgaa gggaatcgtc aaccccctcg aaggagatcc aacggaaaga accgtttcaa
    20581 atagtatgcg ctgcccttcc tggatgtact gccccatcga atgcagatcg gttgtaaagc
    20641 cgacacctgc cgggaataat ccttcgccgt ccttaccttc gctttcacca tacagttgtt
    20701 tccaccattc ggtaaggtac tgcagcgagg gttcataatt taccaggatt tcaacctgtt
    20761 ttccctgccg gtaaagggta ttgcggatag cggcgtacat ggctgcataa ttttcatcca
    20821 ctgaagatga ttccagacaa tgcttccgca tcccgacagc ccctgccacc agtttcctga
    20881 tatcgaatcc tgctacatca atgggtaaaa gcccgaccgg cgtcagtacc gagaaacgcc
    20941 cgccaacatc gtcgggtatt tcatatgttt tataaccttc gtcatccgac agttttttca
    21001 aagcgcccct gcttttatcc gtaatagcaa tgatgcggct tttcgcttct tccctgccgt
    21061 actttttctc catgtgattt ttcaggatcc tgaaagccac agcaggctca gttgtagttc
    21121 ccgatttgga aatcacggcc agcgcataat ccttttggtc aagcaaatcg atgagcctgg
    21181 catgataggt ctggctaagg ctgtttccgg cataaacgat gacggggctt gttgattttt
    21241 catcttttat catcggatga aacggatggc tcaaggcttc gattactgcg cgggcgccca
    21301 ggtaagatcc cccgatacca ataacaacaa acacttcggc tttctgacgt atctgttttg
    21361 catcattttc gatgctggta agcaattcat cgtcgatttg atcgggaagg tccacccatc
    21421 ctaaaaaatc actgcctttg ccggtcttat ttaccagcat ggaatggcac tggtcaatct
    21481 cttcctgaaa ggctgacaat tcttcatccg caataaactg gatgagatta tctttattta
    21541 cagataaatc cgtcatgtgt catgaattaa atttacaatg ggcaattata cctaagaggt
    21601 accataaaat tacgaacatt tacgggaatc ccctaaaaaa aagtgcggcc ttgctagccg
    21661 cactttccaa acaaacaaca aaaccagtta acctttaaaa acccacaagt gtgaattaag
    21721 cggtcattaa aatgaaataa tataacttaa ctaaccgcag caaaattaac cacactaaat
    21781 cagacacgtt gttaatgaaa tataacgaac gttaaaattt gttaaccttg ttaaggcatt
    21841 catacatgta gacttacctt tgtcagcaat gaaaaaaagc gctttcatat tatttattgt
    21901 catcgtttcg ctggcgctgg caaccctgat cgggatgcag gtttactgga ttcagaatgc
    21961 agtcaggctc cgggaaggta attttcagcg cagcgtagac gaagccgtta acgccgccgt
    22021 cctgaaactc gaatacatcg aattatcaag acatataaca cagggagaag ggatcatcaa
    22081 acaggaaagc gattcgtcct tattctatta ccagaaacgt ttggttcccg gtaccgggaa
    22141 tgaagaggca gataaagccg gtagcatttc tgatgccaac gatacgctgg tgaacggagc
    22201 taacctgatc acaattgacc cccgccggaa tgaaccggcg ccgccgccaa aacatattgg
    22261 caacaatgaa ccatccccaa ggcattccgc gttgggaatg caagagttgc tcagaccggg
    22321 acccgaatgg tcgcaaccct ccattatcca ccggatgttt tctcaacttg tcggccctgc
    22381 cggcgttcgc gaaatagaaa attatttgtc cgtggggctt atcgataccc tgttgacgtc
    22441 cgaacttgcc gtaaagggca tacctattga ttttgaattc ggagtataca atacctaccg
    22501 gaataaaata gttctcgaaa aaaccggccg gtatcacgac attttactgg gccgtagttt
    22561 tacatttcct gtttttggca gcgcaggtgt gtcgccgaca aattacctgc ttgtttattt
    22621 tcccgatcaa tcgcgtttca tcctgatgaa cctgtggggg ttactgacca tttccgccgg
    22681 cttgttgttc atcattgtaa tggcttttgc ctactctatg tttacaatca tccggcaacg
    22741 aaaactctcg gaacttaaaa atgatttcat caacaacatg acacacgagt tcaaaacccc
    22801 catctccacg gtttctcttg cgtgtcaggc gcttaccgat aatgacattc ctcatacgcc
    22861 tgaactttac aacaactaca tcaacatcat cagcgaggaa aaccgccgtc ttggccaaat
    22921 ggctgaaaaa atcctccaga cagccatcct ggaacaggga aagcttaacc tgcgttttga
    22981 aaaaataaac cttcacacac tgatccatga tgtgatcaaa aatatcggca tacaggttga
    23041 aatacgcgac ggcagcattg ccaccgaact tctggccgac gacccttacc tgaaagccga
    23101 taaggtgcac ctgtcgaata tgatgtataa cctgctggat aacgccaata aatattcacc
    23161 ccgccgtccc accatcctga taacaaccga aaatgccgaa aatggcgtta tactcaaggt
    23221 aaaggacaac ggaattggta tcagcaaagc aaatcagaaa aaaatctttg aaaaactata
    23281 ccgcgtgccc accggcgacg ttcataatgt taagggtttt gggctgggtc tcagttatgt
    23341 taaatttgtg gtggaaaaac acggaggaac catttcggtt gatagtgaaa taaacaaagg
    23401 aagtacattc agcatatacc tgcctggtaa cctaatgaac acgaatgcgt ctgataaagt
    23461 gaaataaaga cggatcaatc attgtcaaca tctttttgcg caatggaaaa taacaagatt
    23521 aaagtattgc tcgccgaaga cgaccggaac ctcggcaata tattacggac atacctcgaa
    23581 gccaaaggat atgccacacg gcttgccgtc aacgggcagg aagcctttga cgctttttcg
    23641 aaggaaccgt ttgccttttg tatcgttgat gtgatgatgc cggtaaaaga cgggttctcc
    23701 ctggcaaagg atatccggaa aatagatact tcggttccca tcttattcct taccgccaaa
    23761 tcgcttgaag aggacaaact gaagggtttt caggccggcg gcgatgatta ccttaccaag
    23821 ccattcagta tggaagaatt gctggcccgc atggaggcca tcttacgccg tacatcgcaa
    23881 agtcaggata tgcagggagg aaagggattt tttgaaatcg gacggtttgt attcgattat
    23941 aaccaccaaa ttctggttat caacggccag gaacaaaaac ttacctccaa ggaagccgag
    24001 ttgctgaaac tcctgtgcga ccacgccaac caggttgtcg accgcagttt tgccctgaac
    24061 cgtatctggc aaaacgacag ctattttaat gcccgaagca tggatgttta tatagccaag
    24121 ctgcgtaaat acctgaaaga agatacttcc gttgaactga tcaacgttca tggcatcggg
    24181 ttcaaactgg tcaccaatcc cggagcctga tcaacggcgc ttacattcaa gagttccctt
    24241 tattgatgat tttcagcgct tccttccggc tgagcgccga aagggtatgt gaaccgacaa
    24301 atccggcaac ggcttccgca tcggtttttg aatattgccg caatgcccag ccgacggctt
    24361 ttcggatgaa gaaatcctgc tcgcgcgata gtctctcaca taatccgaac aagagttcct
    24421 tatcggtatt ttccccgtat ttcagttgaa acagaacggc tgtccgccac agccataagt
    24481 tatctgtctt taaccattca tcaacggttg gaatgatcct ccccgggttc ttccggaaca
    24541 ggtttcctgc aatattcgag gctatataat ccaccgtgtc ccaccacgat ttactggtta
    24601 tcataaagac agccgtttcc ataaacctga aatcaccttt ccgggaaacc gccgccagta
    24661 tttccatagc ggcatactgc cattcgcgct gaggctgctg ccaggcatat tttacaaaat
    24721 catcggtttc cccgggcgcc ggataaccgg tttccttttt caacgctgcc agaatttcca
    24781 tcctgcgtgg cgaaggaatg ccgtaaaaat cgaacatgtt gcgcaggtaa gcttttgccc
    24841 cttcggcgtt ttttatatcg gaagcagctt caaatttctg tcgtaaaggt tctgtatacc
    24901 gctccagttc taccggatcc atcaactaat aaattattgt gattaaatta tttaacaatc
    24961 aaaagcttat gtacagccgc ttctgttccc ccttctcccg aaaggtgtac aaaatacaac
    25021 ccgggggctt cattccgggt gtcccaacgg gtagtgttgt ttccggcttt aatcatgaac
    25081 gctttcaccc ggttgccggt cgaattatat atagtgaccc tgcagggtga agggcttgtc
    25141 gtctcaaaac tgcatgaatt acgggcgggg ttcgggaaaa tgtgtagatt ccgtaactga
    25201 tttttctcag tctgaccggc atattgcgca tcccaaaccg caagggtata ttcaccacgg
    25261 gcgagcgttt ctgcttcaag gaacccgatg ttccatccgc cgatttgcgt aaaaggcacc
    25321 ccataccatt cataagccgg cccgggacga tacagcagaa ccagcgaatc ggccgaactg
    25381 agcaacaggt tattgtccag atttgattta ctgtaaaaga actttgcttt tgctttaaat
    25441 ccttccggaa aaatgccttc tactttccaa taatgggaat cggaaagacg caatcctgtt
    25501 aaaggcgttt ttaaactgtc cggctccacc cagttgtgtg ttatgcggac aaaggcagaa
    25561 tcaggcacag ctccggtaat aacttttaca tacgtgtcgg ggaattccag ttccccggta
    25621 gtttttatcg ttgcggcata atcagtggtg gcgtccgaaa ttttctgatt aaggtcggcc
    25681 atcaccgcaa cgggttcgaa aggaacgctg aaaaccttgc tgccagttaa acctgaaaaa
    25741 atcaacgtat cggaatactg ttgccactgg tcattcataa aagtgacctc caggtggttt
    25801 gaattagcca gcagggtagt tcccttcgac ttctgcctta cataaacggt cacatcgtaa
    25861 tggccggaaa caggctgaac cgaaaatgaa tcaactgaaa aatgcggaaa tcccggtgta
    25921 aacacccagg tttcaaaaaa atcagtcaga tcaatcccag tataattgcc caggaaatcg
    25981 cggaggtttt cggacgacat atcatgatag gcatactcat tcaggaaagc tttcaccccg
    26041 ccgaaaaaca aactgtcgcc aaggtagttc cggagggtat gaaccacttg tccacccttg
    26101 tcatacaccg tgcttccgta agtaagaccg gagggtattc cgtaaagtgc ataataacct
    26161 ccatccgtga tatgtgtgga ctggagcaca tttttcagtt tcgatctcat gtcatcctta
    26221 taggcagatt cgccgtataa tccaccgaga agcaatgact cgcaccaccg cgcccagcct
    26281 tcattgatcc acatatcttc tgccgaagca caggttacct tgtcgccaaa ccacatatgc
    26341 gagagttcat gcccgtacca ccattctaag gaagtatttc cgttccaacc cgaataaggg
    26401 taagaaatgt ttgaagcatg ctccatggcg ccctgagccg tagacgaata accaacccgt
    26461 tcaaaaggat aaggcccgaa atgggtttca aaattctgca ggatatcgtg gaggtgaaca
    26521 aacgtggctg aaatttttga agtatcggca ggacgtgcat accaggtgat gggaaccggc
    26581 ccgctcatgc cgttgtaaat atcggttttc aattcgtact ggccggtagt aaaggaaatc
    26641 aggtaggaag gaagcgtatg ctccgttttc cagtgccagg tacttgtgtt atcgccatga
    26701 tctgtgatat cgacaagatt gccgccgcca acggcttttt tatcgttgct tactctgata
    26761 taaacatcat ataaggcgcg atcgtgaaaa tcatcaatac agggaaacca ggccttgcca
    26821 aggttatgcg gatcagaatc aaatcctacg ccaaggttga aaacatattc gcctgaaaaa
    26881 tgcaccccgc cccaattcga tggatcggta aagggggttc cgtgatagta aacggttgca
    26941 gttgctgtat ccccggtgtt catcacctga aggaggggaa ttctcagcaa agtcccgcta
    27001 tgactgtagt ttgggattgc cgaaccattc atgataacgg aatccaccgt caggcttatc
    27061 agctccagcg gcaatgctga aataccgttg ataaccggcg tgaattgtac ctcggtgtaa
    27121 ccttttaacg tcttaagcgt cgaattgatt tcggtaagat gaataccata atgcacaaca
    27181 tcaatggtat cactgtacaa acgaggaact gcaactggcc ttggccgggc atgactgaaa
    27241 ccagccggga aaatggccag acaaagaaga agtttaaaca ataccgatga tttcattttt
    27301 caaactctat ttcctgaaca ataacttaat aactttgtaa agatatacac aaaatcattt
    27361 tcagccgttt attcagcgac agcatgccct gaaacaatgc cggaagagct gtttataccg
    27421 gttaacgtga tacgcctatg aaaaaaatat ttctcctgct ttgtacgctg ttggcttcag
    27481 ccacaggatt tccgcaacaa gtctggacgc tcgaaaagtg cattgagtat gccctttcga
    27541 acaacatcca ggtaaagcaa cagatattac agattgatgc aggaaaatca tcgcttctgc
    27601 agagccgcct cgatctttta ccttcgctca atgccggtgc ccagcatggc tataacttcg
    27661 gacaaaccgt ggaccgttat accaatgatt ttgccacaaa aagtgtacag accgataact
    27721 tttatattgg atcggaactg acgctttggg atggtttcaa aaaagtcaac caggtaaggc
    27781 aaaaccaagc tgatttggaa gcggcccgct acgaaaccga taaatttatg gatgatatct
    27841 cgctgaacat agcaacggcc tatcttcaga tactttatta ccaggaagtg ctccgcacgg
    27901 cgcaaaacca ggccggaatt tcacaacagc aaaccgatcg tcttgtaaaa atggttgatg
    27961 ccggcgcttc ggcccagggt gacctctata acatccaggc ccagcttgca tcggcaaaag
    28021 tatcggttgt ggatgcccgc aacaaccttg atctggccta tttaacgctt gcccaaatgc
    28081 ttgatttgcc ctcggctgaa ggatttgaga tcgaaatacc caatctggat atcggaaacc
    28141 aacctgcact ggttgccaat ccggaccagg tttatggata tgccctcgaa acccagcctg
    28201 tgatcaaaag cgccgaactc agagtaaaaa gttcggaata cggactgatg ctggcccgtg
    28261 gcgcacaatc gccgcaactg agcatgagcg cttcgctggc aaccggttat tccggcgcag
    28321 caaccatgct gggcgattcc atcaaaaaaa ctgaaccgat cggattcacc atgattaacg
    28381 gcgtgccgga tgaaatcgtt tatacttcct acacctcatt caaatctaaa gacaaaccct
    28441 tcggggatca gattaacgac aatatcaacc gctccctcag ctttacattg tcggtcccta
    28501 ttttcaacgg atgggctacc cgctctgcca taagccgcgc ccgcattaat gttgaaaact
    28561 cgaaatacac cctcgacctt aggaaactgg aacttcgcaa aaccattcaa caggcatatg
    28621 ccgacgcccg gggcgccctt gataaatata atgcttccct tgcaggtgtc gaagccgccc
    28681 acgaatcttt ccgttttgcc gaacaaaagt tcaacgtcgg aatgatgaac tccgttgatt
    28741 ttaacaatgc gcggaaagaa tacgaacaag ccgactcaga catgctgcgt gcaaaatacg
    28801 aattcatttt taaaacaacc gtccttgatt tttacatggg aaaacccata accctaaacc
    28861 gcaagtaaat tcctgattca taaaatcaac catacacaaa ggacaattaa tcccgaaata
    28921 atatgttcaa gaagaaaaac ctcatccgca tcctgataat tctcggagta ttgttgatta
    28981 ttggcttcgc cgtggccaag aaaaaaggcg ttatcggtaa accggatgtt acaaaggttg
    29041 ccaccgaaat ggtaaaaaaa cgtaccatca ccgagactgt ttccgccagc ggtaaaatag
    29101 cccccgaagt ggaagtaaaa ctctcaccgg acgtttcggg cgaaattatt gaactactcg
    29161 taaaagaagg cgaccaggtg aaagccggcg actacctcgc aaaaattaac ccggaacttt
    29221 atatttcggc caacgaccgt gctattgcag gattaaacac tgcaaaagct aacctggcca
    29281 atgcaaaagc ccgttttgcc caggtaaaag cgcaattcgt caatgcaaaa gcatcgtttg
    29341 agcgcaacag taaacttttt gagcaaaaaa cgatctcaca ggctgaattc gacaatgcca
    29401 aatcacaata cgaagtagcc gaagctgaag tttcagcggc tgatgaaaat gtccgcgctt
    29461 cagaattttc ggtaaacagc gcacaggctg gcgtaaagga agcccgtgaa aacctgacca
    29521 aaactaccat ttacgccccg gtaggcggaa ccatttccaa actcaatgtt gaaaaaggcg
    29581 aaagagtggc aggcgcttcg cagttctcgt ccggcaccga aattttaaga atcgctaact
    29641 tacagaatat ggaagtaaat gtgagcgtga acgaaaatga tattgtacgg gttaaccttg
    29701 gcgatacagc cctggttgaa gtagacgcct accagaaccg aaaatttaaa ggactcgtca
    29761 ccgaaataag cacctcggct aataccacgg gggtaagcgc cgaccaggtt actaatttcg
    29821 atgtgaaaat ccgcatactt cccgattcgt ataaagacct tatcgatccg tcacatccca
    29881 ataaatcgcc tttcaggccc ggtatgtctg caacagttga tatccagacc gaatcggtat
    29941 ccaatgtgct tgctgttccc atccaggctg ttaccacaag aatcgacagc acgaaaataa
    30001 aacaggaaaa cgctgaaatg aaagataaaa aatctcagca aggcgagggc gataataagg
    30061 aagaaaccaa aaagaaaaac ggtgaaatcc aggaaattgt tttcgtgtat aaagacggca
    30121 aagccagaaa agtgaatgtt aaaacgggta ttcaggataa ttcctacatt actatcatag
    30181 aaggtttgaa agaaacagat gaggtcattg ttgctcctta ccgtacagtt tccaagaaac
    30241 tcaaagacgg ggatgaagtg aaaaaagtgg acatcaaaga cctctataaa agcgaaaagt
    30301 agtaaggaat acgaatcaca aaggcaggct tcagaattct gaagcctgtt ttattttgca
    30361 tcgtactttt gtaaatccaa ttctattttg tttatgcctc ttattttagg gatagaaaca
    30421 gccaccgaag tatgttcagt ttcgctcctg caggacggaa aaatgatttc gtataaggaa
    30481 tcagccggca ataatgaaca ttcccgctta ttgaccccgt ttattgatga agtcctgacg
    30541 gttgccggta taaagccgga taaactggat gccgttgccg ttagcatggg ccccggatct
    30601 tacacgggct tacgcattgg cgtgtcggca gccaaaggat tgtgctatgc ccttgataaa
    30661 ccgcttatcg ccgttggcac cttacaggct atggcggctc aggtaatggc taaattaaaa
    30721 aatgaaatca acgacgaaat ggcgctgctt tgccctatga tcgatgcgcg acgcatggaa
    30781 gtctaccacg ccgtttacaa tcaggacctt aatgagatca aacctgttga agcggcaata
    30841 atcgggactg attttcttgc caaagaaaca actaaccatc agctcattct ttttggcaat
    30901 ggcgctgcaa aatgcaggga catattatca tttcgcaata acattgtatt tctcgataat
    30961 atgggcgcat cagcaaacag tgttgcactg ctggccgaaa aaaaattttt tgagcatgaa
    31021 tttgtcgata ctgcctattt tgaaccgttt tacctgaaag attttatagc cgccaaaccc
    31081 agggttaaag gcttatattc caataacgga taatgttctt tatacccggt taaacctata
    31141 ttcatttctt tttctgcgat ttatataatc attctatgct ccggacagga cattaatatc
    31201 gctgcaggat gattctttat taagccaatg ataatctaac aggttggctg acaaatatac
    31261 atgtgttatt tcacgcacct taggaaatga aatatctctt tttgccggtt ataatagtca
    31321 actgaaatca tatccataat ttttatgaat ctttctataa tataaaaaat tgtgaaaagc
    31381 aatagttgtt gataaaacaa aatgaataat gaacaacatc aaaggctatt ttattgaatt
    31441 aatgtaatat agatggtatt gaatataaaa cattataata attaattgtt cattttcaat
    31501 cactttgagt ttttaaaccc ggatggttaa taacgtcggc attcaccgtt taggggttca
    31561 taatttttac gatttataaa caaattgtga tcaaattgtc attattgaaa agtcaagaga
    31621 aatttattca aagttttaag acagatttgt agcggttatt aacaacggta agccggttta
    31681 tttctgttaa cttattaaat ccatcgttat tcacaaaatt gttaataagt attcaggttg
    31741 ttagaaatca atacataaaa caaaaatatt gtgtttataa attgtgtata aaccggaata
    31801 ttgtaacatt caaaacattt tgccgtattt ctgttaacga tattaacagc ctataatgaa
    31861 tcaacattaa atttttaaaa taatatttat tattaatgtt tatagtaatg gtgaataaag
    31921 gattccaaaa aaaaacctcc cggttaaagg aggttaatca tcagaggtat caagcggagt
    31981 cgaaccgctg tacacggttt tgcagaccgt tgcctagcca ctcggccatg ataccgatta
    32041 tgatgcaaaa ataatgatct tttgattaca cacgacaaac tttaaatttc attttgctga
    32101 atatgtaatg gatgatttaa gtatggctgg ataaaaagaa aggtaaattg ggtctatcgt
    32161 tcgagtgtta tacttacctc tcttactttg ccgcctgcag ccagagctgg attaagtttg
    32221 gtaacgatta ccgtgagatg tttgatttca ggatgattcg aataaacaga atcaagaatt
    32281 cgcctggcga catgttcaag cagatgagat ttctgttgca tctcagtcct tacgattttg
    32341 taaactgact ggtaatttat agtttttttt aaatcatcat ttatctcagc ctgacctgtg
    32401 tcggtatcaa tgactaaatc aaccagaaat cgggtaccaa tcacctgttc ttccctgaaa
    32461 cagccatgat aggcaaagaa ttccattcct ttgatgctga ttatacccat aaagttgcgt
    32521 aggtgtaaat atatcggcaa aagtatataa aaaccggcag ggataataat agctaagtgt
    32581 tgtgaagcga acatgtttgt aacaaataac gagctcaata aacttggtaa agtaatctta
    32641 ccgcagataa aacagaaatc acgaaggtcc atctgaacct taatttcaaa cctgcgtggc
    32701 aagcattaac accaatatat taaacaatgg attaataatt gtttacagat aaaatcaatt
    32761 gaagcctttt gggttactaa tgtattaaaa ctgactattt ttgcatcaac tattaacaaa
    32821 cgctattttg gcggaaaaga agataaagga tgactgaaaa caaccccgat tcattacctg
    32881 aagaaaaagt tccggcgaac tttattcatc agattattca ggaagatatt aaaaacaata
    32941 aaaacgaagg acgcgtacat acccggtttc cgcctgaacc caacggatac ctgcatatag
    33001 gccatgccaa aagtatttgt ttgaattttg gcacagctaa aaagtttgga ggaaaatgca
    33061 acctgcgttt cgacgataca aatcctgtaa aagaagatac agaatatgtg gattcgattc
    33121 tggaagatgt tcggtggctt ggttttgaat gggatggatt gtactatgct tctgattact
    33181 ttgaaaactt atacgaatac gctgagaaac tgattaacaa agaattagct tatgtttgtg
    33241 atctttctca ggaagaaatg agtttacaaa gaggtacagt tacaaagcca ggtaccgata
    33301 gtccttacag gcaaagaaca gttgaagaaa acctgagact tttcagggca atgagggccg
    33361 gggaatttcc cgacgggtca agggttttaa gagcaaaaat tgacatggct cacccgaata
    33421 tgctcatgcg tgacccgatt ttataccgga ttattcatgc agaacatcat aacacaggaa
    33481 ataaatggtg tatatatccg atgtacgact tcgctcatgg gcaatgtgat tccattgaag
    33541 gaatcaccca ctcgatctgt acgctggaat ttgaagtaca ccgtccttta tatgattggt
    33601 ttatagaaaa actggagata tttgcaccac ggcaaatcga atttgcgcga ctcaacctta
    33661 gctataccgt gatgagcaag cgcaaacttc tccaactggt aaacgaagga tttgtcaatg
    33721 gttgggacga tccgcgtatg cctacattat gcgggctgag aagaagaggt tatacgcctg
    33781 aaagtatccg ccgttttgcc gaaatcgttg gcgttgcaaa gcgtgaccag gttattgacg
    33841 tatcattact cgaattctgc cttcgcgaag atctcaataa aagggccgac cggctgatgg
    33901 ctgttctgaa tccggtaaaa ctcatcatcg ataatttccc ggaagggaaa gtagaaatcc
    33961 ttcctgcagg tatgaaccct gaagaacccg aaaaaggatt tcgtcaggtt ccgtttgaaa
    34021 aagaacttct cattgaaaaa gaagatttta tgatagatcc tccgggaaag ttcttcaggt
    34081 tgaaacccgg tggtgaagta aggttgaaat atgcctatat cattaaatgt gaaagttttg
    34141 tgcaggatcc tgtaacagga gaggtgtctg aaatccattg tacctatgag ccggatacaa
    34201 ggagcggaat gcccaacagc aataaaaaag taaaagggac cttgcattgg gctgcaatga
    34261 accattctgt gaaaacggaa gttcgtttat acgaccggtt attcagcaaa gagtcgcctg
    34321 aagaagtgga agagggtcag gattttaaag ctaacctgaa cccggattca cttaaagtga
    34381 taacggcctg ctgcgaacct gcgattgaac agtaccatca gggagataaa attcagtttg
    34441 aaagaatcgg ttatttctgt gttgatcctg attcagaacc cggaaaaata gtattgaacc
    34501 gcactgctac tttgaaagat aactgggcaa aaatctcagg taaagaataa aatttaaaaa
    34561 gagtgtatag attaaaaaat ctttaatact tttgcagtgc aattgtccga tggtgtaact
    34621 ggcaacacgt ctgactctgg atcagaagag tccaggttcg agccctggtc ggacaacaaa
    34681 aaatcctgct tatgcaggat tttttatttt acagattttt ttttgatgcc ggaagtgata
    34741 gtaagcttat actttgatcc tatcaatagc tttctgtata cgctgaatga ttttttcctt
    34801 tcctatcagc gctgctatat ccatcatgcc gggtcctgca tttgttccga caactgccag
    34861 tcggaaagga ttcataagtt tacccattcc aattgatttt tccgcgatat aagcatgaat
    34921 agttgcttca agattttctt ctgtataagg ttcagttgct tttatcagat gtatcagatc
    34981 ctgcagaagg gctggagttt ccggtttcca gattttcaat acaacctttt catcataatt
    35041 ttcaggatct gagaaaaaat attgagattg agtccagaga tccggtttca gaacaaccct
    35101 ttcatgaacc agggcacaca ctttctccac ataatcctcg tcaaaattta tattcttttc
    35161 tatcaggaga ggcctgagaa aagaagccag ttctgccggc gttttcctgg acatatactg
    35221 gtgattgtac caatgggctt tttcaaggtc gaattttgcc ccggatttac taacccgatc
    35281 tattgtgaat gcttcgcaca gttcatccat tgaaaatatc tcctgttcag tgccgggatt
    35341 ccatccgagg aatgccagca tattgataaa agcttccgga aggtagccgc tttcacgata
    35401 tcctgaagat acttctccgc ttttaggatc ggtccagcga agaggaaaaa caggaaaacc
    35461 aaggcgatcg ccatcacgtt tactgagttt tccattgccg tcaggtttca ataaaagagg
    35521 caaatgcgca aattcgggca tttgtttttc ccatccaagg taacgatata acaaaacatg
    35581 caaaggagcc gaaggtaacc actcttctcc gcgaatcaca tgggaaatct tcatcagata
    35641 atcatcaacc acgttggcca ggtggtaagt cggcatgccg tccgatttat acaggacctt
    35701 atcatccagc agggttgaat caacctgtac tgttccgcga atgatgtcct gcaaagttac
    35761 tgtctcattt tcaggaatct tgatccggat gacgtatggt ttgcctgctg ataacttggt
    35821 ttcaacttcc tgattggaaa gcgttaatga attacttaat gaattgcgtg ttttggggcc
    35881 atactgaaag gtttccttac gggattcata gtccttgcgg agtttatcaa gttcttcggg
    35941 agtatcgaag gcataatatg cttttccgct atttaatagt tgtaaggcat actgatgata
    36001 tatatccttg cgatcgctct gcctgtaagg cgcatatgga cctcccgtat ggactccttc
    36061 atcaaaatga ataccgcacc aggtaagaga ttcaatgatg tactgttcag ctcccggaac
    36121 aaaccggccc tgatcggtat cttcaatacg aaggatacat ttgccgccat gtttacgggc
    36181 aaaaagataa ttatacaagg ctgttctaac accgccgata tgcaaaggtc ccgtggggct
    36241 tggcgcaaag cggactctta cttctctcat tttaataaaa ttttattgat ggtgcaaaga
    36301 tacaaatcag aaagcaccct gaagttggtt ctttaaaacg aaatagggag cttattgtta
    36361 cttttgtatc ctgctgtaag agtattcagg aagattgaat accctattaa ttttttggcg
    36421 gaagctatct tttagctata tgcgccttga aagcaaagaa atggaaaaca attattattt
    36481 acttctccgg aaaatcgatg catttatccg gaagtattat aaaaaccaac tgatcagagg
    36541 ggcgatttat tccctcgctc tgttggctct atttttcata ctcataagcg cgcttgaata
    36601 tttctcatgg ttcagtacaa ccataaggac gatcctattt tattcttatc tgaccgcaac
    36661 agcttttatt ttgggaagat ttgtgattat tccactgctg aaactgagaa gaataggtaa
    36721 aataatttca catgaagaag cggccaacat aataggaagg catttcactg atgttaagga
    36781 tattctttta aatacgcttc aactaaaaaa tcagtgtgat gatgaattgg caagccggga
    36841 tcttattgaa gcaagtattg atcagaaaac ggaaaaatta agacctgtaa attttacagg
    36901 agcaattgac ctgaagcaga accgaaaata tctgaaattc gcattacctc cggctattct
    36961 gattcttgcc gtattgataa tctcgccttc tgccgtgact gaaccggcaa ccaggatact
    37021 tcatcataaa agtatatttg aaaaaccagc tcccttcgaa attactgtct tgaacagcca
    37081 tttaaaagcc gtccagcagg aagattttga attgaaaatt aaactcaccg gaaatgagat
    37141 tcccggtgaa ttgtttttaa gaattgggga tgcggtttac agaatggaaa aagaaaacaa
    37201 tatccgtttc aactatactt ttaaaaatat tcagcaacgg gttgtcttta attttgaggc
    37261 tggtgattat aattctaaag aatacacttt ggaagtactt ccaaaaccta ttattctatc
    37321 gtttgatatt caggcggact atccgcaata taccggcaga aaacctgaaa aatttgaaaa
    37381 tgccggtgat attactgttc cggccggtac tttgctgacc tggaaatttt atctgaagga
    37441 ttccaatgta ttgtatctga ggttatttaa taaggtgagg gatttgccta ctaaaaacag
    37501 taatgtgata acctggtcta cgcgggtgaa tgaatctgca ggttactcgg ttatggttgg
    37561 aaatgaacat tttaaaaata ttgattcgct gcaatatttc attaatgtaa tacctgattt
    37621 gtaccctgaa ataagcgtta atgaatatag tgattcgatt tatgaaaaca ggttgtacta
    37681 taaaggtagc gttaaggatg actatggttt tagccgtctg gctttttgtt atacagtgaa
    37741 aagtgaaggt agtaaggcaa ccgataataa ggtaattctt aaggatatat cactggaaaa
    37801 gaatgtatta cagcagccgt tttatttctt tatggatgtt gctgctttat ctttaaagcc
    37861 aggggatatt ttggaatact attttgaagt gtgggataat gatgcgataa accattttaa
    37921 atcaacaagg tcacaaaagg gcgtttttcg tattccaaca gttgatgaaa tagaaaaaaa
    37981 gacggaagag aaaaacgaag atattaagga tcgcatggaa acagcgattt cacaatcaag
    38041 aaagttgcaa cagcaaattg aaaattttga caggaaactc atcgaaaaag atgaaattgg
    38101 ctggcaggaa aagcagcagt tgcagcaaat gattgataaa cagaaacaac tgcaaaatga
    38161 aatagaagaa attaagaagg aaaaccatga caaagcgtta aaggaagaac aattcaaaga
    38221 tattgatcag tctatcatgg aaaagcagca aaagttggag gagttgttcg atcagatact
    38281 tccggacgaa atgaaaaaga tgtatgaaga acttcagcaa ttattggaaa aagtagataa
    38341 agagaaagtt caggacatgc tggaggaaat gaaaatgaac aacgaagata tggagaagca
    38401 acttgaccga aacctggaaa tgttcaaaca gttggaattt gaaaaaaagt tgcaggaaac
    38461 aatcgataaa atcaaggaaa gcgctgataa gcaggaaaaa ctgtcggaaa aaacaggtga
    38521 tgcaaaaagg gaggaacttg aatcgataag cagggagcag gatgttttga ataaacaatt
    38581 tgataacata cgcaacgacc tgaacgatct ggaaaagaag aacagcgaac tttctgaccc
    38641 gaataatctc tcgaatacag atcctatgga agaatccatt caaaaagaga tggacaacag
    38701 taaagagcag cttgaaaaca agagtgggaa aaatgcttca aagtctcaga aaagtgccgg
    38761 tgaaaaaatg caggagctgg ggcagaaact tgagaatatg caaaatgaaa tgaacgagga
    38821 aaacaatgcg gaggatatag aaactttgag gcagattctt gaaaaccttg tagacatttc
    38881 atttggtcag gaaaagctga tgacggaatt aaatacaatc ggagtcaata atccgaagta
    38941 tcttcaggta atacaggacc agaaaaacct gaaagacgat ctgaaaatgg ttgaagattc
    39001 cttattttct ctgagtaaaa gacaatctgc cgttgaaccc tttgtgatga gggagatcaa
    39061 tgcaattgat aacaatatgg aagaaaccct ggatgcttta aatgggcgta cggtaaatac
    39121 tgcgaaatca aagcagcagt atatcatgac ttcagttaat aatattgcgc tgatgctggc
    39181 agaatccatg aaagaaatgc aatcgaatat gtcgtttggc ggttccggaa aatcaaaatc
    39241 acagtgtaat aatccaaaag ctggtaatgg gaaaagcgga aagatgaata gtttacgtca
    39301 gttgcaggaa caattgaata aacaattggg agaaatgaag aatgaaatgg ataaaaacgg
    39361 aagtaagcct ggcaataagg gcagcagtca gatgagtgaa aaattggcaa gaatggctgc
    39421 tcagcaggaa gctcttcgta agcaattaca ggaaattggg gaagaaatgc tgaaagaggg
    39481 ttatggcgac gggaaaagca ttaagcaaac cctgcaaaac atggaacaaa cagaaaccga
    39541 tatcgtaaat aaaagaatta cccgggaaac aatgcttcgt cagcaggaga ttgttacaag
    39601 gcttcttgaa agtgaaaagg ctgaacagaa gagagacctg gatgaaaaaa gggaatcaaa
    39661 tgaaggtaag gacagatttt atgaaaatcc tgccgctttc tttgattata aaaaattgaa
    39721 agaaaaggat aatgaaatga tcagggttgt tcctccatca ttaaaacctt tttataaaag
    39781 gaaagccaat gcttatttcc tctcatttga ttaagtatac aatctaaaac aaaaagaatg
    39841 ataaatttta attttcaaaa cactgcttta aagcttcaac aacggcagct tctccgaagc
    39901 tggataaaag aagtaatatc atgggaaggt cttaaaacag gagaagttaa ttatatcttc
    39961 tgtgatgatg aaaatttata tgaattgaat atcaactatt taaaacacaa cacattaaca
    40021 gatattatta catttgatta tacagaaaga aataaaatct caggcgatat ttatatcagc
    40081 gttgaaagag ttagtgaaaa tgcaaaaaag tttgataaga aattttatga tgagttatac
    40141 cgtgtgatga ttcacggaat tttgcattta gcgggttata aggacaaaga cgaaacaagt
    40201 aaaattgcga tgagaaggaa ggaagatgaa tgcctgaacc ttttaaaccg gaaatttgaa
    40261 acaaatattt cggatacgaa ataatgtatc cgaaaatgtt ccacgtgaaa cactaaacaa
    40321 ttgtttaaag aatacgatat aatagttgtt ggggccggtc atgctggttg tgaagctgca
    40381 gcagcagctg caaacctggg gtcttctgtg ttattggtta ccatgaatat gatgacaatg
    40441 gctcaaatgt cgtgtaatcc ggctatgggt ggaattgcaa aaggtcagat tgtcagggaa
    40501 attgatgcat taggaggata ttcgggaatt gtgaccgaca aaacaatgat acagtttcgt
    40561 atgctaaacc gttcaaaagg acctgctatg tggagcccga gagcgcaaag cgaccggatg
    40621 cgattctcgg aagagtggag attatcgctt gaaaagatca gaaatatcga cttttggcag
    40681 gatatcgtca ttggagtgac ggcagaaaag gggagagtaa caggtgtaag gacttcaatg
    40741 gggcatgaaa ttaaagcgaa agcggttgtg cttaccaatg ggaccttcct gaatgggatc
    40801 atacatattg gattgaaaaa attcacagga ggaagaatag gggagagggc cgctcatggt
    40861 ctgacggaaa accttcatga attaggtttt gaatcgaaaa ggatgaaaac cggtacaccg
    40921 gtgagaattg atggaagaag catcaatttc gataagatga ttgaacaaaa aggtgatgat
    40981 aaaccgggaa agttttcatt ttcggaaact caggttctga agcgccaaag aagttgtttc
    41041 atcacttata ccaacactga ggttcatgaa gttctgaaaa ctggatttga tcagtcgcct
    41101 atgtacactg gccggattga aggtatcggt ccgagatatt gcccatcgat tgaagataag
    41161 ataaaccgtt ttgctgataa aacaaggcat cagctttttg tagaacctga aggctgggac
    41221 actgtagagt attatttgaa tggtttttct tcttctttgc ccgaagaaat tcaggtaaaa
    41281 gcaatgaaat taatcccggg actggaagat gccaggattt tcagacccgg atatgccatc
    41341 gaatatgatt attttccccc ggttcagtta atggcgacac tggagacaaa aaatattgct
    41401 aacctgtttt ttgccggtca aattaacggg acaaccggtt acgaagaagc tgccgctcag
    41461 ggcttgatgg cgggaataaa tgctcatctg aactgtaagg ggaaagatcc atttatcctg
    41521 aagagatccg atgcatatat cggtgtgctg attgatgacc tgataaccaa aggggtggat
    41581 gaaccttaca ggatgttcac ttcgcgggcc gaataccgta tacttctgcg gcaggataac
    41641 gctgatttga ggcttaccga aaaaggttat catcttggac tggcatcaca ggaaagatac
    41701 aaccgcgtaa gggaaaaaat tagtgctgtt gaaacgttaa ccaactacct gaaaaatgaa
    41761 agtatttctc cggacctgat caacggtttt cttgaaaagg ccgggacagc ccctgtgaaa
    41821 caaaaagtaa aactgtcatc tctgttgttg cgtcctcaaa ttggattgca agcgatgatg
    41881 gaaataattc cggatctgaa tgattttgtc accggaaaat tgcacggcga tgatgaaata
    41941 actgaagaag cggaaatact gcttaaatac gaaggttata ttgaaaagga aaccgagatt
    42001 gcccaaaaat tgtcgaaatt tgaagatatt gcgttgaagc cggattttaa ttaccgaagt
    42061 ttgacggcgc tatcctggga agccagggaa aaactttcaa aagcaaaacc ggcaaccatt
    42121 ggacaagctt cccggatcag cggtgtttcg ccttctgata tttctgtact gcttgtctat
    42181 attggccgat gaaggttgtt ccacgtgaaa caacataatc attactcaca aaataaattc
    42241 agggcatatg acccaactca tcaataacat ggaaactctt caaaattgtc cggtttgcgg
    42301 aaacgaatct tttcatccct gtatttcatt gtccgactat ttcctgaccc gggaagattt
    42361 caagattgtt gaatgcgaca aatgctcttt ccgttttacg aatcctcgtc cttttagttc
    42421 ggaactgggg aaatactacg aatcgaaaga gtatatttca cactccaaca ccaggaaggg
    42481 gcttttcagc attttatacc agaacgtccg caaatatacc atacgttcaa aattcagaat
    42541 aatatcggga ttgacaaatg gaaattcgtt gctcgatatc ggatgtgcca caggtgagtt
    42601 gcttcattat tttcaaaaaa agggatggga tgttaccgga atagagccga atgacaatgc
    42661 ccggaaattt gcgcatgagg aatatgatct cagggttgag gatgaagact atctgaagaa
    42721 aattaatacg gcttcgatag acgtaatcac catgtggcat gttttggaac atgtatctga
    42781 tttaaacgga aggcttggcg aaatcaaccg aatattaaat gaggacggaa tcctgatcat
    42841 tgccgttccg aattccggct caccggacgc agaatactat ggtaaattct gggcaggtta
    42901 cgatgtgcca agacaccttt accattttac aaaaccaacc atgcacgatt tactccggaa
    42961 acacggtttc agttgcataa aagaatttcc aatgaaattt gatgcctttt atgtaagcct
    43021 gttgagtgaa aaatattttt gtggaaaaac ccggtggata gcggcatttt tttcaggctt
    43081 gcgctcaaac cttaaagcaa gaagggaaaa taactattcg agcatgattt ttgttgcaaa
    43141 aaagagcaaa atttgaaata aggcctttta atggaccgaa ggccctttgg gatagtttat
    43201 cccaaaaaag aaaaaaaagt gatttatatc gctttatttt aaagaggctc tgaaacgcct
    43261 gtcattgttg ggaaaatcgc attttatcat gatgaagcac gatttattca accgtcgctc
    43321 taaaattttg gcaattgcgt tcgcattgtc attgtcggcg gctttgatca taacgggttt
    43381 tgtccggtat caaaaccaac ctgcaaaaat tgttgagagg ttgacccggc aggtagaaca
    43441 ttccgctcaa atgctaaacc tggagattgc tgttttacaa actaagttga cagaaccgga
    43501 agaatcagta caatcatacc tggaaaatca ttattcatca tattttgaag aaaaaggaca
    43561 gttattcctc atttatcaaa acaacaaact gatatactgg actgacaatg cttttcctgc
    43621 gccggaaatt tttgacacta accaatttgc cgcccctgtg attaattcgg gaaacgggtg
    43681 gttcctggcc cggtctgaaa ggagaggcaa ttttcggatt ataggactgc agttgctgaa
    43741 gtataattat aaatatcaca acgactatct tccggattat ttctttcaga aattccagat
    43801 tccccggaat acagaattat caccattaaa cgattctctg catgagaacc tgtacaaaat
    43861 attgtcttcc gacggtaaac cacttttctg catatcgttc gacggcgtac cccgtccggg
    43921 cccatgggcg attccgatcc tgttcatatt ttataccctc agcctggtta ccttaatagc
    43981 gctgatgttt tcactttacc ggactgttta tagcattctt cgttcaaact ggttattgcc
    44041 ggcatttttt ctcgatgttt taattatccg gtgcctgcag ttttatttca ggtttcctgc
    44101 tatcctttac gaatctgact tgttcagtcc tgtttattac gcttcatcag acattctgcc
    44161 ttcgctggga gaccttttta tcaatatggt cctgatcctg cagatctcat ggtacactta
    44221 tcagggcctt aaccgtagct cacggccggt gaatttaaaa aaaatcccgc gaaatatcat
    44281 tctggtgacc ggcgtgattc tgaccggatt cctgtttcat ggtatgaatt tgttgatcca
    44341 aagcctggta ataaacagca atattccttt caacctggaa gggcttttca aactatccct
    44401 attatcaatt acaggtttca cggttgttgc cggaataatg cttgcatttc tttacttctt
    44461 ttattctttg agccggtgga tctatcatgc aggaaattcc ccgatatctt ttcttattgc
    44521 aggtttactg gtttcagtat tttatgctgc ctatattctg gttatcggtg aatttaaccc
    44581 gtattattcc ctgtttcttg ccgtgctttt tgttaccttt tatttgcagc accggaaaaa
    44641 accgatcggt tttattcaat tatcgcaaac cgtcctgttc atattcttac tggctatact
    44701 cgccaattat tccttaaacg tatataataa gcatcacgaa ctggagaaga ggaagctctt
    44761 ggcgctgcac ctcaccgaat accgcgacag ccttgccgag tataagttca gtaaagtaag
    44821 caacgaaatg gtttttgatg ataccttgtc gcagcagctt cgaaaagtac aggatcatcc
    44881 ggaagctgaa caggatgtta ttaattatct gacaaatacc tatttcaatg gttattggtc
    44941 aaagttcagg gttcagattg taatttgcta ttccgggaaa aaacttgaga taaaaccgga
    45001 aaacaccatt gtggagtgtt gtgattattt caatgatatt attctgaaaa acgggaaaca
    45061 aacctctgta tccgaccttt attttttcga taacgccctt gatatgatgt actacaccgg
    45121 gcgtatcacc cttgatccgg ctttgacggg attgaaaatg cctttcttca tttatgttga
    45181 aataacatca aaaatcaatt atttcgccct tggctatcct cagcttctgc ttgataaaaa
    45241 catttcagaa ccggaaaacc tctttgacta cagctatgcg atctataaga agggggaatt
    45301 agtaaaaagt gcagggaagt attcctacga tattcatgaa ttatctgaaa gaggttcccg
    45361 gggacgatcc gataattata attacaaggg ttataatcat caggttttta caatcgatgg
    45421 taattcaaac ctgataatca gtcaccataa gctgtcagtt tcggaaagta tagctccctt
    45481 ctccttcatt tttatttttc tggctgtatt tatgttgatc attcaaatga cggatggcag
    45541 atctttggcg ctgaagcggc ggaaaatttc atttatgatc aggctccagg ctgcactggt
    45601 gggtattatt ctggcttctt ccgtggttat cggaactgtc accatactga accttataaa
    45661 gttaaatgaa acaaaaaaca tggatattgt caaggagaaa atgaattctg ttaggtatga
    45721 attggaaata ttattatcag attatcagca tattaatttt caggacgaaa acctgctttc
    45781 agacgcgctt gaccgtattg ccaatgcatt gtttatcgat gtaaattttt ttaacgccga
    45841 tggcggtctg atttcttcat cacgcaggca gatatttgat gataaactca tatcaacaca
    45901 aataaatccg gaaagtcagt acaatttgct gatcaaccac aaatcctttt attatcacac
    45961 ggaaaccatc ggcaattaca gatttctgtc ggcctatgct accgtgcgga attatgataa
    46021 caaagtaatc ggcattatta acctccctta tttttcgaga caaaaggaac aaaagcaaga
    46081 gcttttaaat ttcctggcca cctttgccaa tacctatatc atattgatag ccattgctat
    46141 atttctggct ttattcatct cgcggtatat aacaaagcct ttacagctta tcaggtcaaa
    46201 gttgggaaac cttcaactgg ggcgacaaaa cgcaaaaatt gaatggcagg ggacggatga
    46261 aataggagac ctggtttcgg aatacaaccg gatgatcgat gaacttggac aaaaggccga
    46321 attactggcc caatcggaac gcgaaagcgc ctggcggcag atggcgcgac aagttgccca
    46381 tgaaattaag aacccgctga cgcccatgaa gttaagcatc cagcacctga tgcgggcttg
    46441 gaacgacaaa gcgcctgatt gggaacaacg gctcaggaaa ttttctcaaa tgcttgtgat
    46501 gcagattgat acgttatcag cgattgcgac cgaattttcc gattttgccc aaatgcctga
    46561 acctgagata caacgtattg accttgtccc cctgctgaat cattccgttg atctgttccg
    46621 gaatttttca gctattacga tagctttcct tccatccgtt gaccattgtt acatcaacgc
    46681 cgatgaaaac cagatgctga gggttttcaa caaccttatt aaaaatgctg ttcaggccat
    46741 acccatcgga aggaatggaa acatccggat aacactggaa acatcggaca ggaattgtct
    46801 cattaaggtg accgacgatg gtgccggtat attaccggaa caaaaggaca aaatcttttc
    46861 accgaatttc acaacaaaat cagcaggtat gggtctgggc ctggctatgg tcaagaacat
    46921 cgtagatatg tcggggggaa ggatctggtt tgattcaata cctgacgatg gcacaacttt
    46981 tttggtagaa ttgccgcttg ctggtggtta atgcagcagc aggtaatttc tatacctttg
    47041 taacctaatt tctgccaact tgaatccgat aaagcaactt gccggccaaa cggctattta
    47101 cggtcttagt tccattgttg cccgcgttat caactttttc tttgttccgc tctacacccg
    47161 tatcatcagt actggtagtt acggtttgtt cagcgagtta atggggtata ttgctttgct
    47221 tcaggttgta ttaacgttag gacttgaaac cggatttttg cgctatgcca acaaagagaa
    47281 agataaatca ggattattgt tttcgacagc cctcgttacg ctgattgctg tttcaatgtt
    47341 gctgctggcg gtggtatggt ttgcagcccc ttcgctgggc aaagccatgg gacatcgccc
    47401 aatctatctc atctatgtag cacttatttt agctttcgat gccatcaccg ccatattttt
    47461 tgcccaactc cgcttcgaga accgggcatg gacatttgcc ctgttccgtt ctattaaaat
    47521 catttcggaa gtagtcttca acgtgttgct tttttttgtc gtaccacctt tcctggcaag
    47581 gcattccggc tcgttcctgc tgaattttat ccctgcaagg cccgattacg ggtacattct
    47641 ttttgcaatt atgctgagtg gtgtggtggc gatggtttta tttcttccca ggttggtgag
    47701 gacaagcatg aaattttcgg cttcgatatg gaaaaggctg atggcgtatt cgttacccct
    47761 gatggtcgcc ggattgccgg gagtagccaa cgacttcatc gacaggccaa tgttccgctt
    47821 ctttgctccg gatacttcac cctggcagga tcagcttggc gtatataatg ccgtgataaa
    47881 actggccgtt tttatggttt tgttcgtaca gatgttccgg tatgcagcag aacctttctt
    47941 ttttgcatca gccgagaaaa aagatatccg gaaaacctat gccgatgtca tgaaatactt
    48001 tgttgctttc tgtatcttta tatttctggg catcgtgctt tataccgata tttttgccct
    48061 catcctcggt aaagattacc gctcgggaat gggggttctg ccaattgtgt tgatatccaa
    48121 tattctattg ggtattgcgt ttaacctttc catgtggtac aagcttacag atcacacccg
    48181 atacgccatc tacatcaccc tggccggact ggcggtaacc tttctgctca acgtgttcct
    48241 tatgccggta tatggctatt acgcttcagc ctggggccgc tttttcagtt acatcgtaat
    48301 gatagtaatc agctactggc tgggtgccaa atactacccg attccctatg atctcagggc
    48361 gatattcatt tattttgccg tcggactggg tatttatgga ttgagtactt tactgaacgg
    48421 acaatcctta tggatcagga tgccggttaa cactgttttg ctgttggtct atgttgcctt
    48481 tgtgctcaaa ttcgaaagaa ttagtgtacg aagaatttta tccgtgatcc cgattcaaaa
    48541 attgaaacat aaaagtaaac catgctgata aaaattgtca accaatcgcc gtatccgctc
    48601 cctgaatatg caaccgtcca ttccgcgggg ttagatctgc gtgctcatat ttccgaagaa
    48661 atccggattg aaccgttgca gcggttgctt gttcctaccg gcctgtttat agaattgccc
    48721 gaaggatacg aagcccagat aaggccgaga agcggactgg ctataaagca cggtatttca
    48781 attgtgaata cgcccggtac cattgatccc gattatcgcg gtgaaattaa aatcatactg
    48841 gtaaaccttt cggatgctgt ttttacctta caacccggtg aacggattgc tcagatggtt
    48901 gttgccaggt ttgaacatat aacctggaac gaggttgaaa cactcaacga aacacaacgc
    48961 ggggcaggag gtttcggcca taccggaaca gcctgaaaac aaaggataaa gatgttgtct
    49021 tccttaaatt gaaaccgaat gcaaaaaata tccatactgc tccttatgtt tttcctgaca
    49081 gtagcggttc atccgcttca agctcagcac aggcacagga gagataaaaa aaacaacagg
    49141 acggaaacca gtctggctgc tatcgaccgc ggtgcggtta cggctgtctt tatcgacgct
    49201 accaaagcca agatactggg tgatctgccg aaagcgttgg cgctctatca gttgtgcatt
    49261 gaaaaggatc cgaattccga tgcagcctat tatgaattag cccaacttta ttatcagcag
    49321 ggcgactatt ccactgccgc caggatgacc gaaaaggctt ccgagattga cccgtcgaat
    49381 gtttggtata aattacttct caatgatgtc tataacaaga ccaataacag ggaaggttcg
    49441 ctcaggatta tgcagcagct tgcgaagcaa tatccgggaa atatcgaata cctgtacgac
    49501 ctggcgacct cgtatcttat gatcaataag ccttcggaag ctgttgaaac ctatgatcag
    49561 attgagggca ttatcggcat cagcgaggaa atctccatgc agaagttgcg aatctatcag
    49621 attcaaaaca aacctgaaaa ggcagaagcc gagattctaa ggctggttga ggctttccct
    49681 gataataaag tacgttatta ttcggtgctg gccgagtcgt ttatggctgt cggaaaggag
    49741 gataaagctt cagaatacta taagatcatt gcagaagctg atcccgacaa tccttatatt
    49801 cacatctcgc tggctgattt ttacaaaagg aaaggagaca ctgcacagtc ctttgaagaa
    49861 ctgaaagcag gatttgccaa tccgggcctg gatattgata caaaaatccg ggtgctgacg
    49921 acatactata cgatacagga tatttacgtc aaccggaaag atgaagccct gcaactggcc
    49981 ggtatcctgg tcagggcgca tcctgcagag ccgcaggcgc attccctgta tggtgagctt
    50041 atgctccagg acaaacaata cctggctgcc cgcaatgagt tccggacgct gatttccatc
    50101 gacagcagca aatacgccgg atgggaaagc ctcatgcagg cagacgcttc gttgcaggac
    50161 tgggacgcgc tgatgaatga aagttcacgg gcgctggaac tcttcccgtt gcaaccggtt
    50221 ccatatcttt tcaaaggcat tgctttattg cagcttaaac aacccgggga agccaccacg
    50281 atcctgaatt caggggaaaa actggtatcg gggaatgatg cattactgtc gcaattctat
    50341 tcatccctgg gagatgcata caaccagatc aaagagtata aattgtcgga tgaaaactac
    50401 gaaaaagccc tgcgtattga tgccaacaat tcctatgtgc tgaacaatta cgcttattat
    50461 ctctccttgc gcggagagca gttggacaaa gcggaaacca tgtcaaggaa aggcgccgaa
    50521 cttgaccctg ataatcctgc caatctggat acttacggct gggtattgta taaacttggt
    50581 aaatttgaag aggccaaaac gtggattgaa aaagccgtca acctcaatac taaagatgat
    50641 ccggatttgc tggaacacct cggggatgta aattttaaac ttggcgatac aacaaaagcc
    50701 ttacagctat ggcaaaaagc caaagatgcg ggaggtaaca ccccggaact ggaaaggaaa
    50761 ctgaaagaca aaaaaccaga tgattaatag aaacaatttc gcgggacttc tttttattgt
    50821 ggttttaatg gctgtgtcat gtacacccgc tcgcaaagcc attcgcaccc cactgaaaga
    50881 ggaaggggct gaatacctgt ttcagaacct gaaacaccac gaactcaaat tcgacacgtt
    50941 ttctgcaaaa tttacggcta ctgttattca gaaccggaaa aagacttcgt tccagggaca
    51001 attgcggata caacgcgaca gcataatatg ggtttcggtg actccgatgc ttggaattga
    51061 gatggcccgg tttttactga ccaatgattc catcaagtac ctcaacagga tcaattctac
    51121 ctgtttacaa aaaaacttcg gctatataaa tgagatgctc aaccggacta ttgattttga
    51181 tatggcgcag gccttcctga cgggcaatga tttttcgttt tatgagaaca gccgttttaa
    51241 agcgcaggtc gacgggcagg aatataagct gcataccatc aacaggttaa aattgaaacg
    51301 tatggtaagg cgcagcgatc ccgatatcag tatcccgata cagaccatct ggctgaaccc
    51361 cgacaatttt aaaatcaata aagtggtcct gaatgaaacc gataaagata accggcgttt
    51421 tacggccctt tataaaaaat ttgaaaccac agatagtcag attgttcctt cggaaataga
    51481 ataccgggta gaaacgaatg acgataattt aaaaattgac atcctctatt cgaaaatagt
    51541 catcaatcct gatcagttat ctttttcgtt cagaattccg gaaaattata ctgaggttca
    51601 ggagtggaaa cagaagtaac ccggaacaat attgccatgc tgtcattcct gcattctaaa
    51661 aaattcagtt tcatcgccgg ggttctcatg ctcatactgg tgggaacgga tgcaatgttt
    51721 ggccaaaagg ataaaactgc gcttcaacga cgtaaagtaa aaatagaaag ggagatcagg
    51781 tacacaaacc agttactgaa cgaaaccaaa aagagcaaac aatcgaacct taaccaactt
    51841 tatctgatta ataaaaaaat aaaccggcgg gaggcgctca ttcaggaaat cggcacagag
    51901 gttactggaa tcgataacga ggtagtccgg ctgaacgata caattgataa actttcggtg
    51961 actctggcgg gattcaaaaa ggaatatgct gaactgattt acaacacgta taaaaaccac
    52021 aacgcttacg accggctcat gttcatcttt gcatccaaag attttaacca ggcttacagg
    52081 aggttaaagt atatgcagca atataccgat taccggaaaa agcaggcggc aaaaattcag
    52141 gaaacgcaac tgttgctttg gaataagaag tcgcagtatg aaaagcagaa atcctcaaaa
    52201 ttattgctga ttcataacca gcaaatggaa aagcagcaac tgaccattga gcaggatgaa
    52261 aagaacctta ctataaaaag ccttacaaaa caggagaaga atctcgcacg caagctccag
    52321 gaaaaccagg cagctttgcg aaagttgcag gcggctattg aagcggtggt tgccgaagaa
    52381 gtaagaaagg caaaagcaga aaatgcccgg aaagcagccg ccgaagctgc aaaatcgacg
    52441 gtaaaaaccg gtacaaaaac agcttcaaaa ccggcagcgg gaacaaaaaa aatcacaccg
    52501 ccggtttctg cccctcccat catgtccact acaaccgaag aaatcagcct gtcgaaagat
    52561 tttgccggca accgtggaaa gttgccctgg cctgtcgatc gtggagttat tgccgtatcg
    52621 tacggagaac atgctcaccc tgaatttaaa aacataaaaa taaggaataa cggcattgat
    52681 attgttacaa ccgccgaatc aagggccaaa gcagtatttc cgggtgtggt ttccagtgta
    52741 atttccattg tcaatcttaa ctatgtcgtc atcatacgcc atggagatta cctgactgtt
    52801 tactcaaatc tgaaaagcat ttcggttaaa aaaggggata aggtaaagat aaagcaggat
    52861 ctcggttcca tatttactga tcctgaggag aataaaacca tgcttcattt cgaactttgg
    52921 catggaacgt cggttcagaa tccggaatcc tggctctcaa aataatggtt gatccttaat
    52981 gaattacagg taaaataaac gtcagaaaca atcgggttcc ggtttcagtc tggcattatt
    53041 tgtatctttg tggcaataaa tcaggaaaaa acgcattaaa taaataaagc gaataacatg
    53101 agcaagcttg gaccctggga aataattctg attgtagcag tgatactgtt actattcggc
    53161 ggacgtaaaa ttcccgaaat gatgcgtggc cttggccgtg gagtgaagga attcaaagac
    53221 ggcatgaaat cggatgacga tacttccaaa ccggagaacg gttcaggaaa gtaatcaaaa
    53281 acggcgcatt tcagcctgta attcggaaac tccccggatt tgttttcctg tttttatgct
    53341 cctgatgagc gatccggcat ttttggtcgg ttacaacggt tgttaacaca ttttaacaac
    53401 cggttgtgaa ttaaatataa ataaccgctg taaaatcccc tggttatttt atatttttat
    53461 gacctgctaa cgtactggaa ctgattgtta tgaaataacc tgtaaaaaca tattaaatca
    53521 tttattgcct ctgatgaaat attttgctgc tgtactctta atgctcttcc tcctgccggg
    53581 ttatgtctcc ttagcccagc agtcccaacc ggtcagtgat ccggcaggct cggccgctac
    53641 acccgttatt gtttatgatg atcctatgat tgcagcgctc gatagcctga gcaacctgaa
    53701 atattttgag aacaaccaga aaatgagggc tcaacttgcc cagaacaaat ataattttct
    53761 gcccaacttt gtacctacct atcccgactc agtttattat tatcgcctgc agcggcttgg
    53821 tgtcgagtcg ccgatgggcc tgacgttcaa cccttacgtg aaaaatttca tcgatctgta
    53881 cgccaaccgt cgccgggggc tcatggaacg cgtaatggga ctttcgcaga tttattttcc
    53941 catgttcgag caaaagctta acgaatatga tctgcccctc gaactcaaat acctggccat
    54001 cgttgaatca gccttgcgtc ccgatgcccg ttcacgggtc ggggccagcg gcttgtggca
    54061 gtttatgtac ggaacaggta aagtgtatgg ccttcaggtt agttcctatg ttgatgaccg
    54121 ttgcgatcct gtaaaatcaa ccgttgctgc ctgtcgtcac ttccgtgacc tgtacagcac
    54181 atataacgac tggctgctgg tgcttgccgc ctacaattca ggcccgggaa atgtgaacaa
    54241 agctatccgt cgttccggcg ggaaaaagac attctgggaa ataaggcaat ttctgcctgc
    54301 cgaaacgcgc gattatgttc cggcttttat tgccgtaaac tatgtgatga actacgctgc
    54361 cgagcacaac ctgtatcccg ttattccccg tctggactat aacgaaatag atacagttgc
    54421 tgtacacagg ccgatggact ttacgatcct ttccgaaaag cttaaaattc caagggaaga
    54481 acttgagttc cttaatccgg gcttcctgag aggtttcata ccggcaacgg atataaaccc
    54541 ttattatatc aggttacccc ggaaaaccgt cgccgatttt gtaaacaacg aacaggcttt
    54601 gtatgcgttt tatcagttga agccttatga accttttcag acgacctcaa acgatgaatc
    54661 ggagtacatc ctgaaaaagc attcacataa ggtacggaaa ggggaaacca tcaatactgt
    54721 ggctaggaag tacagctgtt cagtaggcga tatcaagaga tggaaccggt tacgcagtac
    54781 ccggctgaaa tatggacagc gcctggcggt ttacacccgg gttaaaaatc ccgattatga
    54841 tccgaacctg gccctgaaag ctaaggcggc gaaaaacgaa aagcaggaac tcatggtgtc
    54901 cgacagcctg agcagcgccc tggcggatgc cgataccctg gaaacggatg acgaacagat
    54961 agcagggaat gcagcgccat tgcaaaaacc aaaagccgtt acctcaagtg tgaaaagtga
    55021 aacccggcaa acggccaaac cggtttatca tatcgtgaaa aaaggtgagt atcttacaaa
    55081 gatcgccgca agccatcatg ttaccattga tcagataaaa gaatggaaca acctgaaaaa
    55141 aagcacgctt gccgtgggtc agaaacttcg ggttaatgct cccggccaac cggcgctggc
    55201 tgccaataat accgattctg tccaaaaagc aagcccgtca aaaaccagcg ggacaaataa
    55261 aaaggctgga ggccggaatg tcatttatac agtacaggct ggcgataccc tgtggagtat
    55321 agctcaaaag tacgaaggcg ttactgttga ccagattaag gagtggaata atatttcaaa
    55381 aggaagcagc cttaaggtag ggcaaaagat aaaagttata ttgcccggtg gttgatcctg
    55441 aaattattta ataccggtcc ggctgtttga aattcaaatt ttcttttgac atttgaagct
    55501 taaacagcct taattttttt ctgactatgc gaaaaataat ttttcctttg tgcctttccc
    55561 tttttatagg ctttatatcc gggtgcggaa gccccaggag cgggaaatca aatgccaccg
    55621 gtaagagttc cgaaatactt gtggtatgcg aaaaaagtca atgggatggt ccgataggtg
    55681 aagccatcag gagtgttttc ggagctgaga tggaaggact gcccgaatcg gaatcccgtt
    55741 ttgcgatggt taacatacca tacaacagtt ttacaaaatt cctgcagccg catcggaata
    55801 ttttcattgt cgatataaat ccggaatatg ggaaaaccaa gtccaccgcg aggcagaata
    55861 tttggtcgca cccgcaacgt gtcgtcgaaa taaaagccgg gagcgattcg gcttttatac
    55921 gatatttcag gaaatacggg gaagctatca gcttgctgta tgaacagaat gaaaggaatt
    55981 gttatcatgc cgcccattct cttgaccggg atgtaaaagc agaatccatc attgaaaaag
    56041 aaacaggcct caaaatgatg attcccgctg gtttctatct ggcaaaaaac aaaaatggat
    56101 tttattggtt acggaaagaa actaccgaga tgagtctcgg tattatgata tatacttatc
    56161 cctataccga tacttcacag ttttcatatt caaggattta cgctatgcgc aataagtaca
    56221 cgatggataa tattcccggt cccaccgacg gatcctacat gcttatcagc gatgatgtca
    56281 tcaaaccggt atcacggcgg atacagttta acaagaaatt tgctgtagaa acaagaggtt
    56341 tatggtacac caaaggcgat ttcatgggag ggccttttat caattttact gtacttgatg
    56401 agcccaggca gcgtatagtg gttcttgacg ggtttgtgta ccggcccaac aaggaaaaac
    56461 gtggttatat cagggaactt gaatccattc tctgggatac cgaattttca ggtaatctag
    56521 atgtcaaaca ggctgaaaag aaaaagtagc tacccgtttt aagggatttt gtgtagctga
    56581 cggaatgcca aaaaataccg gcagtctctc gcacatttgc agaagcgcag ctaaatcatt
    56641 tctattccgt ctataaatca aaatgagcgg ttgtgccggc agttcgtgtt cacgggaaga
    56701 atgaatcctt tccggcgccc gggcttttcc tgtgcaaatt aaatgcgttt ttgcggcatt
    56761 attcttcaag catccccagt atttgaaatg catccttcag gctttgccgc acagggatat
    56821 aggaatagcc gaataagtct gtaaacttct gagctgaata ataagtcttc gaaaccatag
    56881 gacgggcaag ttcccgggtg aatagcggct tagttcccgt aacgaaactt aacagggcgc
    56941 taatgcgcca taaaaaccca agagcaacgc tgctgacagg gataaagggc ttcttcaggt
    57001 ttaagatttc tgccccaagt gagaaaaaat cacggcacga aagcgtttcg ctattgatca
    57061 gaaaacgctg gcggctgata ttgctgttca ttagtaaaat catggatgcg accacatcgc
    57121 gaacatcaac ataaccgttt atccctttac tgtaaaaagg catgaggcgc ttcatattcc
    57181 ggagcagact attggctgtc acactgtgac aaacggcgcc cagtatgacc gacggattga
    57241 caatagctgc attcagccct tcttcaattc cgcgccatac ttcacgttca gccgaatatt
    57301 tgctgatggc atagccgctg ttttggcccg aaattttcca tactgaattt tcgtcaacca
    57361 gatttccatt ttccggctgg ccgagcgcgg aaacggaact tacgtgaatc agttttctga
    57421 tgccgtgttt caggcagagg tttacaatgt tggctgttcc ttcgctgtta accttcatca
    57481 tgcgtgcgtg atcgcctttg caaaacgata ccagggcagc acaatggtaa acctggtcga
    57541 tacccgtcaa tgctgaatca agggccgaaa tatcaaggat atctccttca acccattcga
    57601 tgagcgacat ctggtgatct ttgtccgggg tgaggcaaga gaaaaatttg ccgatccgcg
    57661 atggtccgtt tttatcccgg atcagaaccc gcacccgttc accttttttg gtcagctcaa
    57721 ataaaagata cgagccgact aatcctgtac ctcctgtaac gaatatcata aatttcagta
    57781 cgttaggtta tccttggatt tacaaaggag atgattttat cggttaacta tctcaacagc
    57841 tttgagaaat gttttgtcgg tacgcaggaa aatcgggtag aaaccggcgt cattaaagat
    57901 gttccgggca ataaacgctt tcagaagttc cgagatgcgg tatcgggagg ccgccaatga
    57961 acggggagaa gcctttacgc cgttttttcc agcgtacgcg acatattcac tataaagcgc
    58021 cggcgtgatt ttaaatgcct tgtcgaattt cttccaatcg gcgtatctgg tgatttcggc
    58081 ccggtgacgg tcggaataat cgaatgcaaa ctggtacaaa atacctttgt tggctgtttc
    58141 gttaaagaat atcaacgagg agtctttttc aagcgggatg aaaacatcgg gcatgatgcc
    58201 tcctcctccg taaaccgttt tgccttttgg cgtggtgtat ttcagtgaat cattgagctt
    58261 cacactgtcg gcgctttcaa gttccccatc ggtaaaacgg tggtagtatt ccatataata
    58321 atcttcgacg ccgccggaat aggacttttg aatgcaccgg ccggtagggg tgtaataacg
    58381 cgccaccgtg agcctgagag cggaaccgtc gctaaaattc atttgttcct gtacaaggcc
    58441 tttgccgaat gaacgccgtc cgataaccgt accacggtca ttatcctgaa tagcgcctgc
    58501 cacaatttca ctcgccgatg ctgagttttc gtcgatcagc acaacaaccg gtttatcgtc
    58561 ccactcgcct tcggaagtgg cataggccga ttgttttggc ctgttgaggc ccttggtata
    58621 tacaatcagt tttttggccg gaaggaattc gtctgacacg gcgatggctt cattcaggaa
    58681 gcccccggaa ttacccctga gatccagaat tacctttttg attccctggt ctttaaattt
    58741 cttcaggact tcagccagtt catcatgggt agtagctgaa aatttgctca atttgatgta
    58801 tcccacgttc tttgaaacgg gatatgaaat atcaatgctc caggtaggaa tttcatcgcg
    58861 tacgatggta aaatcaatca gactgctgac gcccttgcgc ttgatcgtga cttttacctt
    58921 ggttcctttg gggcctttca gcaatttcat gacctgctca ttggtaatct taacatgagc
    58981 gatattcttt ccgtcaactt tcacgatccg gtcgccgccg cggattccga gtttctctga
    59041 agggccgcct gtcacggtgt tcatcacgat cacggtatcg tattcaagat ggaattgaac
    59101 cccaatgccg tcaaattttc ccatcagggg atcgttggct tcattgaaat cggatgccgg
    59161 aatatactgg gaatgcgggt cgaggctcag caacatcccg ttgatggctt cttccttcag
    59221 tttttcagca ttaacggtat cgacataatt ctgtttaatg taactaatga tctgttcaac
    59281 agaatttttt gaaggtcgtt ggaatgagaa tgcatccgaa acaagcgaaa cccgcatcag
    59341 gtagtatccg atgagcaatc cggctgccag ggttaaagca aatgcaaccg gcagataaat
    59401 attgggacga cggttatcag tcataaatga ttaattgact aaaagttagc aaacaattac
    59461 ttgtattttt cagcagtttt cctgatagcg tccggcgacc acatggtgaa catgttaatg
    59521 actttcgacc ggtcatatcc tttgccgtta tcctcaagca gggctgaatc ctgggtgtgg
    59581 atgcgctttc cgttgccgtc gagaatgacg aacacgggga atccgaaacg ctgcggataa
    59641 tccagcccgg ccagcacatc gcggttttca ttttctcggc tgaagtttac gagcactact
    59701 tcataattgg tttttaccag gctgtcgagt tgggggtctt cggctatgag tttatgaaac
    59761 cgcaggcacc acgggcacca gtttccgcca acttgcagga atacatgttt ccctgatgcg
    59821 gaagcacgtt caacggcaag ggcaatttca tgtttcgcat cagccgacgg gttataaatt
    59881 tttacaggct gttgcgcagt ggcgatgaaa aaagtgctca ggaaaaaggc aagcagacaa
    59941 aggtccttca tgttcatatc tttagaaact gtaaaattag ctaaaatccg tcatgggcgc
    60001 catccccggg gaaagatcgg tcattaccgc ttaagctgct tgcgcagttt gtacctttac
    60061 cgggaattaa tattgataca accatgcata ttcagcggtt cgctctcata ctaatctgtt
    60121 tacaggcagt gatatcaata cctgtcataa acgcgcagac tgccggaaat gttctacgtt
    60181 tcggcgttgt gacggatgtt cattatgccg gaattcccga taaaggaaac cttacttaca
    60241 gccagtcgct gataaaactg cagtcgttcg tggataccat gaacaggcta aaacctgatt
    60301 ttgttattga actgggcgat ttcaaggata tggattccct gccgatcccc gcaaaaacgc
    60361 ttgtctatct taaccgtgcc gaagaagtat tctcaggatt caggggcccg cgataccatg
    60421 tcgcaggtaa ccatgacgag gactgcattc cgaaagggaa atttctttca ctggtcgaaa
    60481 atacaggcat cagcaaagat aaaagctact attcgtttga tgttaaaggt tttcactgta
    60541 ttgtactcga tgcctgtttc gattcttcgg gagccgggta taacaggggc cattacaaat
    60601 ggcatgatac caatattccc ggtgaagaat tgtcatggct gagaagcgac ctgcgtgaaa
    60661 cccgtttacc ggcgctggtt tttattcatc agcgcctgga tggttccggc aattattttg
    60721 taaaaaacgc atcccgggta cgtcgtatgc tcaggcatca ccacagggtg aaggcagtat
    60781 tccaggggca ttatcatgaa ggcagcttcg gatgtataaa cggtatatct tattacacgc
    60841 tgaaatcgct ggttgagggg aattcaaagg gaggatataa cttcgctttg gtggaggtag
    60901 atgaagccgg aaatataagt atttcaggat tcggcgaagt gaaatcggaa acactgcgct
    60961 gccccggcgt gctttgagaa atggaaaccg ttacttcttt taaaattaaa acctgcccgg
    61021 ttatataacc gcgcaggttt taataatggc agtgaaaggg tgcttttttt gaaccgctag
    61081 aatggcaagt cgttcacttc ggagttgtcg ctgttcgatg gttcgtcaaa agggctgatg
    61141 ttggaaggcg gcgcatcctg ggttgctacg ggagcatggg caacagatgc cgattgtttt
    61201 tccactttcc aggcgcgggc ttcggtgtac cagcgttggt tatattcgcg gctttcgagg
    61261 tcgaagaaca ccttcaccag ttcattttcg gccagggcag cctggtcgat ccggtcgccc
    61321 caaagtgaaa aacaaacttt tttctgaaat ccgccggagt caatatcaat gacaaattcc
    61381 tgcttgcgcc attcgccgtt acgtccttgt ccggtgacaa ggggcaatat cctgcttact
    61441 tttccggtga tatccataga atgataatat ttagttcagg atgctaaaat agtttttttt
    61501 cgctttcggg aatttggtta atcgggctat gttgaaaaat tcagttttgc ataccggaaa
    61561 tgtatagttt tgacacgcga tcaaaaatcg ggaaacctgt ctttatgttg cggattgtag
    61621 ctgacgataa aataccgttt ctgaaaggaa ttcttgaacc ctttgccgga gttacctata
    61681 taccgggcaa agatataaac cgagaagttg cagccggggc cgatgccttg ctcattcgta
    61741 cgcgtacccg ttgcgatgct tccctgcttg ccggtacccc tgtaaaattc atcggtacgg
    61801 ccactattgg gtatgatcat attgacttcg gattttgtga aaatgcaggc ataacgtgga
    61861 ccaatgctcc cggttgtaac gcctcttccg tgatgcaata catggcctcg gccctgatca
    61921 gcgtttcaaa agaaaagaaa ctttcattgg aaggcaaaac gctcgggatt ataggggcag
    61981 gacatgttgg atcacaggtt gaaaccctgg ccgggttgtt gggcatgaaa gtgctgctga
    62041 acgatcctcc gcgcgccagg agagaaggat gcgaaaagtt cacagaactg aacagattgc
    62101 tggaacggtc cgattttgtg acaatgcatg ttccattgac catggcgggc gatgagaaaa
    62161 cgtatcacct gatggatact gttatgatcg gtatgatgaa acccgatgcc tggctcatca
    62221 atacatcaag gggggaagtg gtggattccg ccgccttgga tgcagcgtta tcaatgggag
    62281 agattgccgg tgccattctg gatgtctggg aaaatgagcc gaaaatcgat tttgaactgt
    62341 taaggaaagt attcatcgga acaccgcata tagccgggta ttcggccgac ggaaaagcca
    62401 acgggacttc ccgggtcgtc agggctttat gcgctcattt cgggttgccg cttgtctcgt
    62461 ggtatcctga aaacctgccg gagccgttaa ataatgcatc catcagaata aatgatttac
    62521 tgacggagga tgccgctatt ttgcatgcca ttctgcatac ttacgatatc cgcaacgatt
    62581 gtacacggct cagagagaac ccggctgatt tcgaaaagct gagaaactat tacccggtac
    62641 gccgtgaatt tcaggcttat acggttaccg gaagcaatct gaagcccgcc gtaaaagaca
    62701 aactggtgaa actggggttt aaggtatctt aggtatcagg gcagctcttc ag
//
LOCUS       DAOPYV010000004        61923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_338_length_61923_cov_11.417825, whole genome shotgun sequence.
ACCESSION   DAOPYV010000004 DAOPYV010000000
VERSION     DAOPYV010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 61923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61923
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_338_length_61923_cov_11.417825"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="PKI35_00815"
     CDS             complement(<1..1534)
                     /locus_tag="PKI35_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX42948.1"
                     /translation="MKIRIYFAANAALFCFFFFSLNTSAQYYQAVKTDATYYYYDSTS
                     MQIISARIDSVAISGTETSYFAMRQIRQTDYGCWTTNGASWLGDEIIEYPDGKTQFIF
                     YPFSPSDSNDVYTIYSQAGIGESWRFYNYHNGLQYIEATVDHIELVDFPELADYVKVI
                     VLNCKDTSGQIVANPINGESLMLGMNYGLLRLPKFDELSYSPKFYSFIGKTNPNAGYQ
                     VPKTLDIFDFQPGDELHVAGYSENYNYGGTYTYSTAIVSRYLSRYDYPSGDSVLYTFE
                     YCSLTYHKNNNGVEDYSYLKDTLDQTVSPSYTPQFDTEPLEPVMIPESGWWTYPFTGM
                     SGNSQYCPEGIPYKAFDYLMMWSDDGNGCLWPVTFDGFCNYQTLYFKGLGGPYYHCDD
                     PTYWGKEYRDLKYFKKGDVIWGSPLACDSLMEVGMKERRDDLSIKLFPNPANDYLTIA
                     APATNQLPAVFQLIDNTGRVILEQRQQSQTEILNIYKIAPGCYYFRWIPKSGDIITGK
                     LIR"
     gene            1694..1882
                     /gene="rpmG"
                     /locus_tag="PKI35_00820"
     CDS             1694..1882
                     /gene="rpmG"
                     /locus_tag="PKI35_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNX42949.1"
                     /translation="MAKKSKEARVQVILECTEHKTSGMPGTSRYITVKNKKNTPERLE
                     LKKYNPILKKVTVHREIK"
     gene            1891..2046
                     /locus_tag="PKI35_00825"
     CDS             1891..2046
                     /locus_tag="PKI35_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HNX42950.1"
                     /translation="MAKKVVATLRTSSGKDFAKVIRMVRSPKTGAYTFKETIVANELV
                     KDFLAKK"
     gene            2212..3162
                     /gene="ftsY"
                     /locus_tag="PKI35_00830"
     CDS             2212..3162
                     /gene="ftsY"
                     /locus_tag="PKI35_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNX42951.1"
                     /translation="MGIFSFFTRENKETLNKGLEKTRTSVFSRLSKALVGKSKVDDEV
                     LDNLEEILVTSDVGVETTLKIIDRIQQRVSHDKYLGTDELNSILRQEIAALLAENDNN
                     NFEGFDFPKRETPFVILVVGVNGVGKTTTIGKLAYNFRKAGKSVLLGAADTFRAAAVD
                     QLAIWGERAGVPVIKQAMGADPAAVAFDTVKAAVNRNSDVVIIDTAGRLHNKIGLMNE
                     LGKIRSVMQKVLPDAPHEVLLVLDASTGQNAIEQAKQFTAVTDVSALALTKLDGTAKG
                     GVVIGISDQFKIPVRYIGLGEKMEDLQVFNKDEFVDSLFA"
     gene            3269..4567
                     /gene="rimO"
                     /locus_tag="PKI35_00835"
     CDS             3269..4567
                     /gene="rimO"
                     /locus_tag="PKI35_00835"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNX42952.1"
                     /translation="MKPHTIRVVTLGCAKNTVDSEKLMAQLNANNIRVIADGDKGRAD
                     TVIINTCGFINDAKEESVDTILEFIEAKKQGKIKDVFVMGCLSERFKGPLQAEIPEVD
                     EYFGVNDLGSVVRRVGAEYRRTLLGERLLTTPKHYAYLKIAEGCNRRCSFCAIPNIRG
                     RHISRPIPEILEEARLLAAKGVKELLLISQDLTYYGIDTHQKQLLPELVEQLSDLKLF
                     SWIRLHYLFPATFPDRLLEVIASRPDVCNYIDIPLQHIESHLLSSMRRGVTREETIAL
                     IKKFRKALPNAAIRTAFIVGYPGETEEDFAELKDFLIYSEFDRVGVFTYSHEEDTHAF
                     GLKDDVPEEVKQQRASEIMEMQQEISMHKNHLKIGKTFKVLIDRLEGEFYVGRTEFDS
                     PEVDNEVLIEVGNKIKIGEFYNVKIKQADEFDLYGEIADF"
     gene            4580..5416
                     /locus_tag="PKI35_00840"
     CDS             4580..5416
                     /locus_tag="PKI35_00840"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HNX42953.1"
                     /translation="MKINCLVIALLLLTANMVFSQTETGSNNKTDASGLKQGTWEENT
                     TTGVEKGTYLNGKKEGTWVSYTNKGNLAKLESFKNGLHEGISVEIDQRGYLLSETYYE
                     NDLIEGVAKRYYYGSNPASVITYHKGLKNGPYKVYYENSAGKIMEESNYVNDIKDGMS
                     KWYTIKGDLIAEYNYVMNSLEGVQKSYSEGNKLLSEQTYKNNLENGPYREFFASGKVK
                     MEGSCTDGKKTGVWKEYTEDGKLFSEGSYVNDLKDGKWVEYDANGKTSKVTKFVKGEI
                     VK"
     gene            5511..5996
                     /locus_tag="PKI35_00845"
     CDS             5511..5996
                     /locus_tag="PKI35_00845"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNX42954.1"
                     /translation="MKVSLNNPFKGREGYNCFGCSPDNAMGLQMHFEMHGEDVHCEWQ
                     PKQHFEGWHNVLHGGIQTTLMDEIASWWVFVNMKTTGVTSSMEVKFKKPVYTDKGPIT
                     LIAKKHEVSRNIAVIFVQILDSGRTCCAESYIHYFTYNEQTARERFNYPGFGEFVKET
                     D"
     regulatory      6248..6452
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6534..8432
                     /locus_tag="PKI35_00850"
     CDS             6534..8432
                     /locus_tag="PKI35_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX42955.1"
                     /translation="MMRKLLILFLIFNALAAYSQQMADSVHRLATVEVIADRISSNSA
                     GMQVRQIDSLTRKLYSATTLSKLISEQSSVYIRSYGPGGTASLTFRGLQTSQSVVLWE
                     GINLNSPTHGMMDISMVPVFILDQISLQYGGGSMFSGSGAIGGSLVLSGKECFSQPLK
                     VQARLAAGSFGNISPAVKISLGNRKFCYSSSLIANSADNSYPYINLDGKRKTFQHAAY
                     QSLGTIHKAEWKISEHQTVSAAGWYQASSRDIPPTMVMSKSMQHQSDRALRMSLQWKW
                     LNAEHRVVSGVALTREYLHYTDGMSGIDAEYRNRTYSSHIEYKYVLTPLLVFDAGISG
                     RLLVAETIYYNGIKNRPEAAGYASVIKSFPSAGWKVAANLRQDAVEGYRVPFCPSLGA
                     EGRLAGRLDARLNVSRNFRVPTLNDRFWMPGGNPDLKPESSLNAEAGLAYRSGETKSP
                     LSWKVELSGYSVLVDDLILWIPSGGSTLWSPQNIEEVWSRGVEASGSLRLTEKKLKSE
                     LSVNYTYSPSTVTCTATALAEKNGKQLIYTPLHTIKSNFMMQFHDYTVQLQNTFESRR
                     FTQEDNRKWLPAHTLLNISAGRVLKIWKKDFTIQLDINNLLNSSYQAVQYYPAPGISF
                     TGSITAEF"
     gene            8458..9531
                     /locus_tag="PKI35_00855"
     CDS             8458..9531
                     /locus_tag="PKI35_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42956.1"
                     /translation="MKKLKLLSWLTLSIVLFISACHKDDPENLKGQFSNGIFIVNEGI
                     FQTGVGTISFIDRSTNMISQDIFAQVNGRPLGSVAQSMTISNGKAYIVVNNASVVEVV
                     DAATFESEGIIGGLVSPRYFMAVSNSKGYVTDWAGNVDGDVAVVDLASNTVTKTIPVK
                     SGPEQMLIANSLVFVLNSGGWSTDSTVSVIDPASDEVLSTIQVNHRPTGIVKDKYGKV
                     WVMCSGMGFNGYPQAGDSKGHLIRIDPVTLQVDFDYVFKSSSEHPEKLVINKNGDRLF
                     FLFKGAINSFDVDNTTEDPVILAMKDNLYALGYDASSDYIYASDPLDYQQNGWVYRFK
                     SADGALIDSIRAGIIPGNFCFGN"
     gene            9569..9985
                     /gene="arfB"
                     /locus_tag="PKI35_00860"
     CDS             9569..9985
                     /gene="arfB"
                     /locus_tag="PKI35_00860"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNX42957.1"
                     /translation="MPLFSENDLLAECVFTFTRSSGPGGQNENKVSTKVELWFDISSS
                     RVLTTDQKKMIISRLKNNATSIRLTSGRERSQMANLKLVSKRFVEKIGKLLEVVPERI
                     ATRPPAASKEKRLSNKRLLSEKKAGRQSKSAGIHED"
     gene            complement(9982..10344)
                     /locus_tag="PKI35_00865"
     CDS             complement(9982..10344)
                     /locus_tag="PKI35_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNX42958.1"
                     /translation="MNHSTLDTIIPDWAKELPASITVTDKNANILFMNDKALLTFAKY
                     GGTDLIGRSLFNCHAESSAAKIRGLLETGGTNVYTIEKQGTRKLIWQSAWFSNGVVSG
                     LVEISLILPPEMPHYIRS"
     gene            10425..10498
                     /locus_tag="PKI35_00870"
     tRNA            10425..10498
                     /locus_tag="PKI35_00870"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10459..10461,aa:Arg,seq:acg)
     gene            11017..12903
                     /locus_tag="PKI35_00875"
     CDS             11017..12903
                     /locus_tag="PKI35_00875"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX42959.1"
                     /translation="MKKIYSLIVLACLTMAVSGQTYLLEDFSGNVMPPAGWSIDGLNS
                     QWSISASNNAGGTAPEAAFTYKNVVTTTRLISPPVDMTGQTSVTLMFNHRYAFYDNPA
                     PAYGVATRSAGGAWTSIWEHTPTASVGPETVILTISNSDMGHSDFQFCFYLNGNMYNL
                     DNYWLDDIMLFQPLSLDASLKSISLPPYVVAGASNTLSGSIMNYGSTNINSFDIAYRI
                     DGGAAQVSSVTGLNLALGESYEFTHSVPIVLNTAGIYNQVEVNIQNVNGGNDLNQNND
                     TLKANVGVLEYAPVRKVFCEEATGTWCGYCVRGICFMDYMAETYPETWIGVAVHNGDP
                     MVNTAWDNEIPNIIPSFPGYPSGTIDRAGSNYWDPSQFEEGYQKRIAQPTPVTVEINN
                     CAWDPATRNISFDLQSEFLLGTDDEMRFAAVIMEDSIHGTDDDYAQANYYAGGSLGAM
                     CGFENLTNPIPAADMYYNHVGREILDSPYGTAGSIPAPITSGSSFTYHYTYNIPADWS
                     FEHLHFAGLVINNTTGEIMNANNVVNWLGTNEPTRVTSLNVYPNPASNNAKVVFDLSN
                     GQQTGLRILDITGRQVASFTQRTYPAGTNTINLDCSDLANGLYIVELTVGNTVFTKKL
                     SVQK"
     gene            complement(13159..14406)
                     /locus_tag="PKI35_00880"
     CDS             complement(13159..14406)
                     /locus_tag="PKI35_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX42960.1"
                     /translation="MKNYLQKITRRMKEGFMNTLKIWLREYQVVLTDVGVVIIIIAAP
                     VVYPILYSLVYYPEIVSDMPVTVVDLSQTSESRNFMRQLDATQELKVEGSCISMEDAV
                     QQFKQRKIRGIIVIPESFSEDIANNRQTNISAYADMEFFLYYKTLMMGANFVSLEEGK
                     QIQINNLMQKGLDLNQAGAVVDPILPDDNSIANAVGGFATYAIPVALMLIIQQTLILA
                     IGIAAGTARERHALGTLVPMDKDKMGTFRMVFGKSAAYFTIYALLCVYLIGIVPKIFG
                     YCRMGSFGELAALMTPFLLSCIFLGLTLSVLFKTRESPMMLFLFTSFPLFFLSGIIWP
                     LSNFSPFWLMFRELFPSSNAIFGFIKMETLGATVFECKKEIMAMWIQTGVYFVTACIV
                     YEVQVRLSKRKRAELASTVAQTE"
     gene            complement(14454..15656)
                     /locus_tag="PKI35_00885"
     CDS             complement(14454..15656)
                     /locus_tag="PKI35_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX42961.1"
                     /translation="MKSLIKSIWAVAKREIDRMSSGTLYILAVLIFPVLTTLFFCSLM
                     KEGVPAKMPIAIVDLDQTSTSRKMARNIDATPASDVVMSLQTEKEAMSELRKGSIYGY
                     IVLPQNLQADIMANRQPVVSYYYHNGLFIAGGLLRNDFALITHTLSAGINIQKREAMG
                     QSEQEIMAQVQPIQLNMHLLFNPRANYTVYVTTIILPIMMQIFILMMTVYSIGIEIKE
                     RTSREWLKLSDKSIVVALTGKLLPYTIIFLILMMFQNFMLYKVMAVPMNGGVGWIILS
                     SFLFVLSYQAIGIFCIGLLPMMRHALVLAAFYAILALSLCGFSFPTESMSPIFQIWSY
                     GFPVRHYMHIFQSQILAGFEPIYSARSIIYLLLFLLLPLIVLNRLKSALIYQNFIEDI
                     HKTDIQAI"
     gene            complement(15739..16752)
                     /locus_tag="PKI35_00890"
     CDS             complement(15739..16752)
                     /locus_tag="PKI35_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX42962.1"
                     /translation="MKTMNKRHSGMLVAFLTLLLVIAVVSIIGWIILKPEPVILQGEA
                     EATEVRVSGKVPGRIEKFNVSEGTEVKKGDTLVFLSSPELNAKLLQATSAEDAAAAQS
                     MKAKKGARAEQIAGAYELWQKAEVGVSLAKKTFDRVQNMFNEGVVPAQKRDEAEANYQ
                     AAIATSKAAKSQYDMALNGAEQEDKLAAAALVNRAKGAVSEVKSYLSETMLQSPIDGE
                     ISDIFPKQGELVGSGAPIMSIVDLNDIWVVFNIREDLLPTIKMGSRFTGTVPALGNKC
                     INLKVNYIKAMASYATFKPTKNNGGFDVKTFEVHAEPVEKVDGLRPGMSILVDYKQFD
                     AKN"
     gene            complement(16799..18313)
                     /locus_tag="PKI35_00895"
     CDS             complement(16799..18313)
                     /locus_tag="PKI35_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX42963.1"
                     /translation="MKTKLLNLTLTIALTALTIGVNGQMIGVSPELRELITLSVSKDY
                     NVANQNIEKEITGVQQKAVRSAYIPKVEVGGKYMFAYSFLDSDIDKITGFESLYQLKD
                     QLNAFMQTPAFPAMFPTLAQLSSEISNLQQLLMQQGIQLPTITDKVAGKLYGNYFGID
                     ASVKMLLYSGGKVPNAVKALDQKIKAQDALTEKCTSDVIGEVITCYDQLALLNQSKKV
                     LDESAARLDAEKKFAYSALNNGMATSFDTLKIAVAEANLNAKMSEYESKKTMLHQKLS
                     QLTGKPAESFENINPELEPLLYAKTNSDINNRPELKALSAGVEAQKFLVKSEKSNYLP
                     KVQAIASARYDNIFNADVDMTDPMKVETKINNLNFGPTFLVGVGFKWDVFDRSAGSSK
                     VRQAQLDLQKAENAKNSATELLQLNQSKAITNYSASVSQVEFKEKQLRAARMALDLAQ
                     KSYNEGMLNITERLAAETEMQNSELEYLQAIFAQRQSVLECYKATGDLTLSNIK"
     gene            complement(18472..19053)
                     /locus_tag="PKI35_00900"
     CDS             complement(18472..19053)
                     /locus_tag="PKI35_00900"
                     /inference="COORDINATES: protein motif:HMM:NF019959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX42964.1"
                     /translation="MLDKDEVKDTIINVARHIFSRFGFRKTTMDEIALATRKGKSSIY
                     YYFNSKEDIFQAVVEKEAELLKNELSEAVSKVSDPREKLKSYILVRMNAFKKLANFYD
                     VMKNDYLSHLEFVNNIRNKYDQEEIMMFENILKEGVSNGEFNIEYTNLAAVAIVTAMK
                     GLEIPLFVEGNEQDIEKRIDNLISILFYGLLKR"
     gene            complement(19325..19861)
                     /locus_tag="PKI35_00905"
     CDS             complement(19325..19861)
                     /locus_tag="PKI35_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNX42965.1"
                     /translation="MNRTFLIIFALILAASGSMAQKAPPKSSEPAIKWYGFEEGMKMA
                     KKKKLPVLVDVYTEWCGWCKRMDKETFSDKNIVRYVNESFIAIKLNAEDKNPIKYLDD
                     TYINPNPSAQRSTHQLPGVLAKQRIGYPTYIYLDEKGKTITLTQGYMQASDMLPILKY
                     IGSGSYKTMTWKQFTGLK"
     gene            complement(19919..20371)
                     /gene="smpB"
                     /locus_tag="PKI35_00910"
     CDS             complement(19919..20371)
                     /gene="smpB"
                     /locus_tag="PKI35_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNX42966.1"
                     /translation="MSTENRKISIRNKKAGFEYYLQEQFTAGLVLTGTEIKSIREGKA
                     NLTDSYCTFDNGELFVLNMHISEYKFGSYLNHEPKRPRKLLLTKKEIRKLQQKLKDKG
                     ITIIPVELFVNEDGFAKLTIAIAKGKKLYDKRETLKSKDQQREIDRHI"
     gene            complement(20425..22869)
                     /locus_tag="PKI35_00915"
     CDS             complement(20425..22869)
                     /locus_tag="PKI35_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX42967.1"
                     /translation="MKLHFLSVVLAFMPLLAFPQFTVSGKITGETGNETLPGAHIMLS
                     GTYNSAITDQTGSFVLRNVKKGIYTIKVTYLGYKDYEEKITVDKSLNLPITLSPTVFV
                     QDEVIILATRAGTNDPVTSQTISKNEIAQGNLSRDIPFLIGNATSVVTSSDAGNGMGY
                     SSLRIRGTDMTRINVTINGIPLNEAESHEVYWVDLPDIASSTNSIQIQRGVGTSTNGA
                     AAFGASINLQTETSDPEPYAVFNSSTGSYNTFKNTLNFGTGLMKNHWNLEGRLSKLSS
                     DGYIDRASVDLQSYYLSGTYTDQKNLIRMTTFSGNEKTYQAWDGIPSSILDTNRRYNG
                     MGMYYDSKGNLRFYDNETDNYLQTHYQLHLAHQFSSKLYMAGSVHYTKGFGYYEQFRD
                     DDELSSYGIEPLRLQQPFYIIGSDTISEPDSLINQCDLIRRKYMDNDFYGFTYSLNYH
                     KKRFQATFGGSWNRYDGNHYGTVIWTQFAGNALKDQEWYRNNGDKSDLNFYSKINYQA
                     GNRLNLYLDLQYRHIDYLIDGIDDDARDITQQHHFDFYNPKAGIYYQANSSNSFYASA
                     AIANREPNRDNFTDADPNQPEPTREKLYNLEAGHNLKTSKAEITSNIYFMYYHDQLAL
                     TGAINDVGSAVMVNVPESYRLGLEISSKWLLSEKLEWIASVTLSRNKIISFTEYIDNW
                     DDWTQIPVEHHNTDLCFSPAVLANSEIIYSPLQKLSFWLSGKYVGRQYIDNTQAKERS
                     LHPYFVNSFQAGYTLKPRICDELSFNIMINNLFNYRYESNAWVYRYYSEGNYKVLDGY
                     FPQAGINVTAGITLKL"
     regulatory      complement(22933..23028)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(23116..23805)
                     /locus_tag="PKI35_00920"
     CDS             complement(23116..23805)
                     /locus_tag="PKI35_00920"
                     /inference="COORDINATES: protein motif:HMM:NF037435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HNX42968.1"
                     /translation="MAREKNTGIGLKFRDAAKDYLYLLEHNYPQKAILKLIGDRYALS
                     SVERSVLYRGISSARESAGRAAKLVSPDSVTGSVLNIDGYNVLITIGSYLNGNLVFIS
                     SDHFVRDASEIHGKAFRNELFNKAILLIFSYLQSNQPREINFFFDKPVSHSGELCEKI
                     NILLNHYEIKGSAITLPSPDHYLKMLDSGLIATSDSSVIEHCKTGIIDLPQAVIRFHY
                     KREMSDIASYL"
     gene            24045..24458
                     /locus_tag="PKI35_00925"
     CDS             24045..24458
                     /locus_tag="PKI35_00925"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNX42969.1"
                     /translation="MKILIINGPNLNLLGRREPEIYGGQSFESYLERLKNMFPEIMIE
                     CFQSNVEGEIINKLQEAGFTYQGIVLNAGGYSHTSVAIGDAIRSISAPVVEVHISNIM
                     AREDYRSRSVISGGVKGIISGFGLDSYRLAVLSLI"
     gene            complement(24546..25568)
                     /locus_tag="PKI35_00930"
     CDS             complement(24546..25568)
                     /locus_tag="PKI35_00930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX42970.1"
                     /translation="MPTVAVVILNWNGREFLRQFLPSVIQHSEGFAEVIVADNASTDS
                     SVEFLQNNFPRIRIIQNITNGGFAKGYNDALSQVNADYFVLLNSDIEVTENWIKPVID
                     LMESDPKIAACQPKLRSYHHTEKFEYAGAAGGFIDKYGYPFCRGRIFQTIETDNGQYN
                     TPSEVFWATGACMFVRAKEYLEFGGLDEDFFAHMEEIDFCWRLKNGGYKVMYCPDSVV
                     YHVGGGTLPKQSSHKTYLNFRNNLALLYKNLPSDLLWPVFLARVPLDGIAALKFLLEA
                     GFADFYAVIRAHGYFYRNFFKLRAKRKKLSHNHVSMIYNGNLVIDYHFRKKKSFPEMD
                     QEKFSG"
     gene            complement(25688..26566)
                     /locus_tag="PKI35_00935"
     CDS             complement(25688..26566)
                     /locus_tag="PKI35_00935"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX42971.1"
                     /translation="MNFKLGIIKAINRLFSLLPFWVIYLKSDFIAFILRRVIRYRRNV
                     VEENLRNSFPEKSTQEREAIARKFYRNLSDIIVEIFKTRGMTAGELQRRMKVVNPELL
                     DHYRRLGRSVIMVSGHCGNWEWYAPATAMQCPGFDHNCALVKPLSDPVSEEFITSLRT
                     RFMKDVLLPFKSAYRVLLSRRRDQNLTAILADQTPHRSEIEYHTLFLNQQTAFFLGAE
                     KIARSLDHVVMFSHIRRVKRGYYEVTFELITDTPKLTRDFEITNKHIALLEKDIIENP
                     DNWLWSHRRWKYKIDN"
     gene            complement(26600..28081)
                     /locus_tag="PKI35_00940"
     CDS             complement(26600..28081)
                     /locus_tag="PKI35_00940"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNX42972.1"
                     /translation="MKKLLVAGTFLFLSVAAMPNPGNLRAFFSYTTFRSPEHGPYIET
                     YLSVLGKSVQYVHKDNGKFQGSILVTMLFKQNDSIRDFRKYELFSPEVDDTTVINFSF
                     LDQQRVALPNGKYDLDISISDKNREMKPFLVTDQFELNYPNEQAAISGIQLIESFTRV
                     EKPGMLTKSGYDFIPYLDNFYPAAVNKLTFYTEMYDLARLLGADEKFIVATSIESFET
                     GKVIKDNLRMKREDVKPVNVVFSEFDISNLPSGNYNLVVAARNKENKEIIRNSMFFQR
                     SNPSLKYDLNDLSSIDIQNSFVSAYTNPDSLREFIRMCFPIASSSEKLFINTQLNIAS
                     LETMQRYFLSFWQSKAPENPSGAWQKYYIQVLAVNDEFKTSIKKGYETDRGRVYLQYG
                     PPNSRVQEYNEPRAYPYEIWQYYKIGSQTNRKFVFYTHDYSSRDFDILHSDVLGELYN
                     ARWEVELHKRDTDRYTSNPQDIERTGEDDYYGGRSRDLWDNPR"
     gene            28237..28324
                     /locus_tag="PKI35_00945"
     tRNA            28237..28324
                     /locus_tag="PKI35_00945"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28271..28273,aa:Ser,seq:tga)
     gene            28382..29566
                     /locus_tag="PKI35_00950"
     CDS             28382..29566
                     /locus_tag="PKI35_00950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX42973.1"
                     /translation="MQFPYRRNLFALYIIKVAKWMNLVMPVIVLFYKSNGMGMAEIFQ
                     LQAVYSLTLMFFEIPTGYFADKAGRKTSMICGSFLGVAGYLIYSTSFGFWNFVMAEVA
                     LGLGQSLVSGADSAMLYDSLVAGRIADKYTRYEGRINSTGNFAEALAGIAGGLLAAGS
                     LRTPFYVQATVAALAIPASLCLVEPPAVGAKIKPTLKNAFAIIRKALHSDRKLKWNTL
                     FSAVTGVSTLTMAWFAQPFFIQAGLSVSWFGIIWAVLNVTAGIASACAWKLEKRFGPS
                     GTVTVFSLVLVSGYLGISFLPSAMGFLLLLMFYFARGVATPTLRNYVNLITVSEERAT
                     VLSVRNFVIRMMFAILGPVFGRITDKYSLPVAMAAAGVLLGTMMGITLYYFLKYRTYQ
                     AD"
     gene            29643..29734
                     /locus_tag="PKI35_00955"
     tRNA            29643..29734
                     /locus_tag="PKI35_00955"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29677..29679,aa:Ser,seq:cga)
     gene            29853..30104
                     /locus_tag="PKI35_00960"
     CDS             29853..30104
                     /locus_tag="PKI35_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42974.1"
                     /translation="MMNFIPIGVGCHSGYKADEYPEYFILEEKRFEILEISDRWYQAE
                     NIPGYPVSDYFEVRTQYGVYLIRHEPAGDNWYLCISDKG"
     gene            complement(30237..30956)
                     /locus_tag="PKI35_00965"
     CDS             complement(30237..30956)
                     /locus_tag="PKI35_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNX42975.1"
                     /translation="MDPQTEKGSLFLFPATLGDVSAETCLPAGNILILNLINVFVVEE
                     IRTARRFLKKAGYTGLIDDITFFLLNEHTSDQEIDAYLEPAAGGKNIGLMSEAGLPCV
                     ADPGHKLVSLAHQKGIRVIPLTGPSSLMLALMASGFSGQHFTFHGYLPVKTPERIKSL
                     KLIEREVRQTGSPQLFIETPYRNMAMFHTIIETCHPDTRLCIAANITLQDEFIRTMPV
                     HRWKSQIPELQKKPAVFILGI"
     gene            complement(30959..31918)
                     /locus_tag="PKI35_00970"
     CDS             complement(30959..31918)
                     /locus_tag="PKI35_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNX42976.1"
                     /translation="MTATVNFPSREEILGAADRIRPYIHRTPVITSETINRICGAELF
                     FKCENLQKAGAFKVRGATNAVMSLPGEDIKRGVATHSSGNHAAALSMAARNRGIRAYI
                     VMPGNSSKVKIAAVQSYGGEITFCEPTLAAREETLNQVLAQTFATEIHPYNDLRIIAG
                     QATAALELIEDAGNLDYIITPVGGGGLLSGTALSASYFGKGIKVIAGEPSGADDAYQS
                     FYKGELVPSVNPDTIADGLRTSLGSLTFPIIRKFVTDIHTVSDFNIAEAMKLVWERMK
                     LIIEPSAATPLAALLKNKTRYAGKRIGIILTGGNVDLNKLPWQ"
     gene            complement(31915..32385)
                     /locus_tag="PKI35_00975"
     CDS             complement(31915..32385)
                     /locus_tag="PKI35_00975"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNX42977.1"
                     /translation="MIKILMVCTGNICRSPMAEGLMREKSKQAGLGVVVDSCGFEPFH
                     LNDPPDDRAIYEMKKHGIDIANHTMRLFRQADFDLFDFIYVMDQSHYRNVIRYARSES
                     DRKKVDYIMNAAGHGNNRHVPDPYYGRQSDFETTYSLLDQATDAIINELSKRNR"
     gene            complement(32631..34064)
                     /gene="dnaA"
                     /locus_tag="PKI35_00980"
     CDS             complement(32631..34064)
                     /gene="dnaA"
                     /locus_tag="PKI35_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNX42978.1"
                     /translation="MKELHSEVWDNCLKVIRDNLNYQSFKTWFAPIKPLKLENNILTI
                     QVPSQFFYEWLEEHYITLLKKTIRNELGPTGRLEYSIVMEGSYTSDPYTIKVPASNRK
                     ATNNPAVAMPIDLKNGNAKEIPNPFVIPGLKKIQVYSQLNESYSFDNFIEGDCNRLAR
                     SAGYAVACNPGKTAFNPLFIYSAVGLGKTHLSHAIGLQVKNNFPEKTVLYVATEQFMQ
                     QFIDSVRNNNQNDFLHFYQMIDVLIVDDIQWLSGKEKTQDVFFHIFNHLHQKGKQLVI
                     TSDKAPVELKGLEPRLLSRFKWGLAADLQVPDLETRIAILRKRIYKDGIDMPDDVLEY
                     LAYSITTNVRELEGALISLMAQSSLNKKAITIELAKQMIDKFVKNTAREISIDFIQKI
                     VCDYFGIQVDSINSKTRKRDIVQARQLAMYFSKKHTKSSLAAIGLVCGNKDHATVLHA
                     CRTVNNLIETDKQFRLYVEEIEKRIKF"
     gene            complement(34303..34917)
                     /locus_tag="PKI35_00985"
     CDS             complement(34303..34917)
                     /locus_tag="PKI35_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNX42979.1"
                     /translation="MLFDDQYHSIEKTAEGLFREKGSRFIAYAIPVITETEVKEQLDI
                     LRKKYYDATHHCFAYVLGNEKSAWRVNDDGEPSGTAGRPIYGAIQSADLTNILIVVIR
                     YYGGVKLGVPGLINAYRTAAKDAIAHAQIITNTVKEVYRIEYPYDLMNDIMKILKEEG
                     PLIISSEFGMTCVIEFAIRRNNLRKLLGRFDRLNNLSINYLKTI"
     gene            35168..36073
                     /locus_tag="PKI35_00990"
     CDS             35168..36073
                     /locus_tag="PKI35_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX42980.1"
                     /translation="MDSHFGEFAALLTTLFWTITALSFEVVGKRIGSLNLNLLRLSVA
                     TVLLGVFSYFYRGLFLPVDATLHNWLWLILSGLIGFVIGDFLLFQAYIVIGARVSMLL
                     MTLAPPAAALAGWLILGESMSLKSLLGMMVTIAGISLVIFNRTPGTSKSSKSARLAHP
                     LKGILYALGAAIGQGVGLVLSKLGMESYDPFAASQIRVMAGVVGFALVFTVAGRWRAL
                     PAAVKDSKSMKWLIVGSFFGPFLGVSFSLMAVQNTNAGIAQTIMSLVPVLIIPPSLII
                     YKEKLRARELAGALIAVAGVSLFFL"
     gene            complement(36078..36557)
                     /locus_tag="PKI35_00995"
     CDS             complement(36078..36557)
                     /locus_tag="PKI35_00995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNX42981.1"
                     /translation="MSKSVYRETECPFCVKEFSEAAFLADDDFLAVYNIAPILPGHSL
                     IVPRKHVRGLSDLNDDETARLFVFAKKTTNLLMAAFHGEGFDWSLQEKEAAGQTVPHL
                     HLHIVIRKTGDLKDIGEWYPRVDHADNHLLDSFSRPKLCSADYRQITAYLRKTASLF"
     gene            complement(36554..37006)
                     /locus_tag="PKI35_01000"
     CDS             complement(36554..37006)
                     /locus_tag="PKI35_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNX42982.1"
                     /translation="MNSLPAINEFLAQPVWAIAGVSRSRNKFGHSMYLTLKKRGRKVI
                     PVNPNLSEFDGDTCYHSILDLPPEVTGLVICTRPEISKGLIADAVSRGIRHIWLQQGA
                     ESNDVLEPYHDSGLNLVSGKCILMYTDPVESVHKFHRFLAKLFGSYGK"
     gene            complement(37015..39501)
                     /locus_tag="PKI35_01005"
     CDS             complement(37015..39501)
                     /locus_tag="PKI35_01005"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNX42983.1"
                     /translation="MKKITFLLAICLLTGITHGYSQRYLPFASSNYAGVTGVYLQPAS
                     IADSRYKFDLTIAGVEGTFNNNYIGLDPYYLLNPKKFSTDWDAEDFDKEDFLSENTSS
                     DDKSMIINFQAETMSFMASVSNKASIALTFGTRGIINLDNMSHELAKIIYESDNYEDY
                     LKQDLRDANVNIQGNFWTEFGFTYSRVILDEGEHFLKAGATVKLLQGVGSFYGFAKEF
                     SYNFLERDSVSIFHTKVNYGASDNFDNLFADNGFNYENLNYKVAANPSLGFDLGFVYE
                     YRPDWESFKYDLDGETNLWQRDQNKYLFRIGLSILDIGSVKYKRGALSNDFTADIANW
                     NYQDQDFGKGYSGFLNTLKDSLNFEFDNSMSDIYRMNLPTAISLQADVRAAKGLYLNV
                     SPYIALNRGSNDVNKVHYLTAWNVVPRYEGRFFGLYLPFQYNSYKQLNVGAGLRFGPL
                     WIGSNNLFSIIAGKKDIYGINASVALKFGIRYKKAHDRDKDKVSDRKDNCPDVPGLAE
                     LAGCPDADLDGVTDAEDLCPNEPGLKEFSGCPDRDGDSIIDSKDDCPDVKGLPQFNGC
                     PDSDGDGIIDSRDDCPFNAGLAELNGCPDQDSDGIADKEDNCPTVFGTRENHGCPFID
                     SDDDGVKDEDDQCPGIKGPVENHGCPYTDTDNDGIPDKDDECPSIPGTAIFRGCPDTD
                     GDGISDKYDMCPTIPGVAQNNGCPEIKKEEQEIINTAFANLEFETGKSVIRRVSYPSL
                     NELAQLLVKHPEWKLQLSGHTDNVGKRETNMTLSKNRTLAVKKFLQDRSVPASCIRAE
                     WFGPDRPVDTNATPEGRQHNRRVEMKIVFE"
     gene            39690..40418
                     /locus_tag="PKI35_01010"
     CDS             39690..40418
                     /locus_tag="PKI35_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42984.1"
                     /translation="MKNSILLVVFLLAGIKGFSQVKSGWRDGGEMIFSWGNINQGNLD
                     ADNVPRFSLILNEQLQYHVDYGQHTGFYTGLGIRNVGFIHKFGDTLKIKQRVYSIGVP
                     FALKLGNMDKNVFFAVGAEMELFFHFKEKTFLHGEKMKTGEWFSDRTELFHPSVFTEL
                     DLGKGFYIRYRYYLTDFLKQKSGSFPGGIHLPYNNSGTLMYISVGTMISKKKAREQLP
                     VIRKASNTYASAYLPGSSSAKQLK"
     gene            40422..41294
                     /gene="prmC"
                     /locus_tag="PKI35_01015"
     CDS             40422..41294
                     /gene="prmC"
                     /locus_tag="PKI35_01015"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNX42985.1"
                     /translation="MSDPENRLMTVGDLAKTLRAMLEPVHGREESGAMVYRLFEHYLH
                     LNRASVVLSRQMFPGKRQQKKLLQAARQLADNIPLQYITGEAFFDGLTFRVNRHVLIP
                     RPETEEMVSLAAGIFAGRRPHSILDIGTGSGCIAVSLKKHFPESRVIAVDVSKMALRV
                     AQRNASDNGLQVEFLHGDILKPHGFRNMPVFDLIISNPPYVTESEKSLMRANVLDYEP
                     GKALFVPDKDPLLFYNAVARFARRFLAGNGMILFEINENFGKATVDLLKSLGYSRAEV
                     RRDFRGKDRFVIAQ"
     gene            41633..43066
                     /locus_tag="PKI35_01020"
     CDS             41633..43066
                     /locus_tag="PKI35_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX42986.1"
                     /translation="MTEKVQKLMKDVPAYRWTALVLLASAMFFGYIFMDILSPLQELL
                     QTSRGWTPDAYGRYAGSETFLNVFVFFLIFAGIILDKMGVRFTAILSGLVMLLGASIN
                     YYAVSDAFTGSGAENAMNHFMNLPLKWWNITPFFSGMPASAKMSAIGFMFFGCGVEMA
                     GITVSRGIVKWFKGKELALAMGVEMAIARIGVAVVFICSPIVALQIKPINASRPIAFA
                     VLLLAIGLISFIVYSFLDKKLENQMGESGEEKDDPFKISDIGKLLSSKVFWQVALLCV
                     LYYSAIFPFQKYANNMLQCNLGYTADQAGRVFFVFPLGAAAITPFLGNFLDRKGKGAS
                     MLMLGAILMIVCHLVFALALPATKGTSVAPVIAYASIILLGISFSLVPAALWPSVPKL
                     VENRYLGSAYALIFWIQNIGLYAFPIIIGTALRSSNPGITNPMEYNYTVPMLIFASLG
                     VLALFLGIWLKAEDKKKGYGLELPNIK"
     gene            43195..44298
                     /locus_tag="PKI35_01025"
     CDS             43195..44298
                     /locus_tag="PKI35_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNX42987.1"
                     /translation="MKSKLILLAVLILLSQIALPSGFQKGETRNKPKHPKNIILMIGD
                     GMGTAQLYAGITALRDNTNIERCPVSGFVKTLSASNYITDSAAGATAYAIGCKTTNGF
                     LSETPDSIPKPTILEMAEKKGLATGLVATSAITHATPAAFIAHVSSRGSYEQIAAQFL
                     NTDIDVFIGGGWKYFTKRADGRNLADSLLKHNYVIARSLEEVEKASGPKLAGLLSDEH
                     MPRYSEGRGDMLPLSTEKAINILSGNKKGFFMMVEGSQIDWGGHANDLNYIVEEVVDF
                     DKAIGKALDFAAKDGETLVIITADHETGGMAITGGDMSLGKVEAKFTTGDHSAVMVPL
                     FAFGPGSERFSGIQDNTDVFKKCVELLQLSDGK"
     gene            44333..45013
                     /gene="mqnB"
                     /locus_tag="PKI35_01030"
     CDS             44333..45013
                     /gene="mqnB"
                     /locus_tag="PKI35_01030"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HNX42988.1"
                     /translation="MKILIVTATWKESEPLRQALGDCIPVENILFRYRLNDKVIDLLV
                     TGTGMVSTAYAMGQLTGRQKYDLAINAGICGSFNRNIRLGEVVNVVADSFADMGAEDN
                     DSWLPMSKLGSENQDDLLSGDGTFNSNPGRFSVLLNHLRPVNGATVNTVHGNAESIRR
                     FSVRTDAGIETMEGAAFMFALTGSDTKFIELRSVSNYVEPRNTDNWNIPLAIGELGVE
                     LVRLLKDL"
     gene            45182..46009
                     /locus_tag="PKI35_01035"
     CDS             45182..46009
                     /locus_tag="PKI35_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HNX42989.1"
                     /translation="MNLNLGFSPCPNDTFIFEALVHQKVDTEGLTFDYILADVEELNQ
                     KAFRKELDVTKVSFHAFLSLTSQYLLLDSGAALGFGAGPLVISRKEMQLSGLRGASIA
                     IPGINTTANLLLSLAVPEAKNKSEMIFSSIEQAVLEGKYDAGLIIHESRFTYESKGLK
                     KVADLGEYWESLTGSPIPLGGIVVSREIPDDIRQKLNRSIRRSVEYAFANSYPLNGFI
                     AGNAQEMSEEVMRKHIELYVNQFSVSLGTEGKQAVLKLFEMALKSGLIKSIPGSIFA"
     gene            46053..47969
                     /locus_tag="PKI35_01040"
     CDS             46053..47969
                     /locus_tag="PKI35_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX42990.1"
                     /translation="MKQNNLIGIFLLQFLAFILPVSGYAQVEKGGMPRSFSLKGLSDE
                     TGIIEITPPDRDSLIAQDYRDALLDKGYRMGVVLPLEISMNNSGNWYSIQGGRIWKLC
                     IRSAGAQALTLYYERFSLPEGADFFVYSKSGQHIAGAFTPDNNPEGNCFATRLIPGDE
                     IVLEYYEPFSVSSVPVIELNGLLYAYRSAFYGSEKNGDDFGGSGSCEVNVNCSEGDNW
                     KDQKQGVVRILTRIGNNSFWCTGSLLNNTSQDYSPLLITASHCALNEYAATFANSNDL
                     MQWIFYFNYESEGCEDPVKEPVRQTMVGAEKLATSYSFDNDRLGSDFYLLRLTGTIPS
                     GYNPFYNGWDCNDVPSSSGVCIHHPQGDIRKISTYTKPVVSSTWESIPGTHWETYWSE
                     TQNGYGVTEGGSSGSPLFNKSGQVIGILTGGGSYCNTPNLSDYFGKIAYSWVSNGTAD
                     TNQLKPWLDPVGTGIKKIAGSYNSTFPIARFNTDTITIPIGSSVTYYDLSVNNPTGWH
                     WYFEGGIPSESYQKYPPAIVYNTYGTFDVKLTVTNEYGSDSLIKEDYLTVSARVFPNP
                     TTGKVTLLLGPGEHPELIISVFNQTGQKILEYVPGNSTSSAMSIDLSGLPASVYFVSV
                     RTGESVQVSRIVKI"
     gene            47994..49100
                     /gene="mqnE"
                     /locus_tag="PKI35_01045"
     CDS             47994..49100
                     /gene="mqnE"
                     /locus_tag="PKI35_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HNX42991.1"
                     /translation="MKGLLDNVNIPPSIRNIALKVLDGIRLTQSEAIELYSETELGFI
                     GALADHVRKQKNGDLVYYNRNIHLEPTNICIYNCRFCSYSQHNSGTSWEYTVEEMLKK
                     VREMGDEITEVHIVGAVHPARDIYYYGDLLRQVRAEKPQAHIKAFTAIEIDYMARKED
                     LSLEEGLQYLKDQGLNSIPGGGAEIFDAGIRKQICEKKSTAETWLSVHETAHKLGIPS
                     NATILYGHIENIGHRIDHLDRLRQLQDRTGGFNAFIPLKYRNKNNELSWIEEATVTED
                     MRNYAISRIYLDNFPHIKAYWPMIGKEMASLALSFGVDDLDGTINDSTKIYSMAGADD
                     KSPSMSVQEIADLCKRSGRIAVERDSLYRILRRS"
     gene            49306..49860
                     /locus_tag="PKI35_01050"
     CDS             49306..49860
                     /locus_tag="PKI35_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42992.1"
                     /translation="MKLRNFLVVVLVFGGLTALNAQPVVKGDKLLNLGIGLGSTLYSG
                     SYYTSTLPPVSASLEVVVKDGLLDNKAAIGIGGYAGLAGYKYDYQGWGYKYTNIIIGP
                     RGYLHYNFLDKLDTYTGLLVGYDIVKATEFGTAPGWNYEASSSGLIWSWFAGGRYFFN
                     DNLAGMVELGYGIAYLNLGVTFKF"
     gene            complement(49972..50229)
                     /locus_tag="PKI35_01055"
     CDS             complement(49972..50229)
                     /locus_tag="PKI35_01055"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNX42993.1"
                     /translation="MSPVVLLCLPADFFDKSTVNLCLSKICFNRECFACGTTRAVMHF
                     IHLDFKTAWHFNKLVVLVVPFLLYLWVGEIRRCLKLLKAAG"
     gene            complement(50282..50623)
                     /locus_tag="PKI35_01060"
     CDS             complement(50282..50623)
                     /locus_tag="PKI35_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HNX42994.1"
                     /translation="MKRIYAKIAALLFLGFFMVGIVQVADAAFPGKQQAKEIVTIQKD
                     VENVVAQPTQLPGSTDDDQLLLIILAFFIPPLAVYLKYNEAGKPFIVNVILTLLCGVP
                     GIIHALIHVLK"
     gene            50684..51568
                     /locus_tag="PKI35_01065"
     CDS             50684..51568
                     /locus_tag="PKI35_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX42995.1"
                     /translation="MKVAWWCVFFLLFCNVAYSQAIELTGAIIIGKSEMMSYRIVYNI
                     NNKNEISGYSIADLGGDLETKSRISGVFNPSKKTLRFAETAILSTKAKLSVSDFCLMS
                     VNGKFEKKGGKNVFTGSFISKSHNDKLVCESGTLILTTTKEIYKAAAQVSKAIDKLPA
                     SDSLTREIKARLNPLKGVENVKTLTSGKSEEYTLTDDTVQFDIFDDKLEDGDIISILK
                     NNTTIIHELKTTNKVQSFRFVIRADEKAVTFTIVANNEGSSPPNTVKIVMTNGKTKEL
                     LISQLAKNQRVKLIFRRK"
     gene            complement(51643..52347)
                     /locus_tag="PKI35_01070"
     CDS             complement(51643..52347)
                     /locus_tag="PKI35_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HNX42996.1"
                     /translation="MKIRIVRTILFTIIVASAGAGFISCSGEKKVTSYPVANLVPKKF
                     GHSIQQPVRKKGNLSQEFFQDKCDSGLQRSQVSLAENKTIATLPPVSGSNHSLIRQYR
                     YGNLSDQAYDDELIAGLETSAETSVRKIKKSTAPDHGIIIPADDTTNTQNQDLADKTP
                     GKKDPKDMNGFGIVSLVTGIVSIIGFPFLAPVAIVFGAIGLNKRQKGMAIAGFVLGIV
                     TFVLYSLLIVLLLSSL"
     gene            complement(52428..53483)
                     /locus_tag="PKI35_01075"
     CDS             complement(52428..53483)
                     /locus_tag="PKI35_01075"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNX42997.1"
                     /translation="MADDSDILDQQAEENDLYEHFRFVVDKGQALLRIDKFLMGRIQN
                     ASRNKIQNAAHAGNILVNDIPVKPNYKVKPLDTISILLAYPPRDTTIIPEDIPLNVVY
                     EDPELLVINKEAGMVVHPGHGNYTGTLVNALAYRFQQEGIVTEEGPYLVHRIDKDTSG
                     LLLVAKNELAQSILARGFFEHKVDRKYIALVWGDFKEEAGTIEGNIGRSLRDRLVMDV
                     FPDGKHGKEAITHYKVLERFHYVTLVECTLETGRTHQIRAHMQHIGHPLFNDERYGGD
                     QILKGTTFAKYKQFIRNCFDILPRQALHARSIGFKHPKTGKEMHFESELPEDMASVLA
                     KWENYTRTDSNETERNS"
     gene            complement(53500..55497)
                     /locus_tag="PKI35_01080"
     CDS             complement(53500..55497)
                     /locus_tag="PKI35_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX42998.1"
                     /translation="MMRYKITIFLLFCLSATFQAFAQEVVSGLEYNITVKNKFCQAGL
                     KCAANYDTVYNTLPFYDDFSRITVWPLPEKWADNYAFINTDYAWHPLTIGVATLDAID
                     ETGALYPEAGSYPFEADKLTSQPIRLDSIFSPVPKAITISDSVYLSFYYQPQGRGAMP
                     AKKDSLLLEFHSPANFDTLITETDTTYSPRWDVIWSSPGGVQVDTFALENHGYFKQVM
                     IPVTDSARYFKKGFRFRFRNYASLANSYVPDWQSNGDQWNIDLVYLNTSRSKYDTAMK
                     DIAFAEKAPSMLSRYQAMPYRQYRKNFLNEMKDTLDISIANIDNVPHNISYGYTIRKD
                     SQQPYEEYDAGNFSIYPYSGYGYSTYAPFAHPPVESFYPIGTNDQQKVVFHSTHFLTT
                     DPNLLIKQNDTISFTQVFSNYYAYDDGTAEAGVGLNGSAGSYAVQFELNVADTLRGIQ
                     LYINQTLSGTNNQYIDLTVWNDYLGAPGQIVGQMKDIIPANADSLNVFQTFWFDTPII
                     VDETSFPGLLFYIGWQQSSIDNLNIGFDRYNDSHDKRFYNVSGNWQMSDEQHAGSLML
                     RPVVGLVNPLSNPELSASSLFTIFPNPVTVDEFNIALPQEWKTDHNIDLELFDSSGRM
                     VYQNSFRPVVSAANLKNGIFLARLVNHDNNRTASQRFIIAR"
     gene            complement(55504..56337)
                     /locus_tag="PKI35_01085"
     CDS             complement(55504..56337)
                     /locus_tag="PKI35_01085"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNX42999.1"
                     /translation="MNFIQFITTRKFVKHFLLSVLITIVLAWIILMILKQYTRHGETV
                     TVPKITGLTLAQAQELESSDDFNYVVVDSVFDLTKKGGTITMQDPLPNSIVKPGRIIY
                     ISVVATVPEKVKMPDLVDLSIRQAKALLETYGLRLGNVSMIPDIAKNMVLRMSHNGSS
                     ISPGTSISKGSTIDLVVGLGTGSGAFQIPFLIGKTRAEAQVELQQLGLLVGDEKFIDG
                     DSLDARVYQQNPAYVYGLTVRSSSAISLTYRSSESFDFDTYIKQLVIDTIQNDSASID
                     Y"
     gene            56519..57511
                     /locus_tag="PKI35_01090"
     CDS             56519..57511
                     /locus_tag="PKI35_01090"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNX43000.1"
                     /translation="MKSKKNIALLMGGDSGEFEVSVASAAVIRRNIDETLYNVYPVLV
                     HAGEWIYTPDEKTGIPVDKNSFSVLTHNERIFFDCAFIAIHGTPGEDGKLQGYLDMAH
                     IPYTSCDLTTSAITFNKYFSNDLALQYGMNVAATVMLRRMESWDTESLIRKVGMPCFV
                     KPNKAGSSCGVTKVYHPEDIEKAVNKAFAEDDEVLVQQFIKGRELACGVFRRKGNIVV
                     LPVTEVIPKNDFFDYEAKYTSGMSEEITPASLTGEEVAECNRITAYLYDKFNCKGVVR
                     FDYFLDNGKFWFLEVNTVPGLSDGSIVPKQAVAAGYSLKEFFGILIEEALAGTK"
     gene            complement(57526..58125)
                     /locus_tag="PKI35_01095"
     CDS             complement(57526..58125)
                     /locus_tag="PKI35_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HNX43001.1"
                     /translation="MREIVFATNNQHKLEEIRNLAGPHFIIKGLSDIGCHEDIPETAG
                     TLQGNALQKAQYVREHFGLDCFADDTGLEVDALDGRPGVYSARYAGNNHDFKANTIKL
                     LNELKGVTNRSARFRTVIAFLTGGDQYFFDGVINGRIIEEMRGKEGFGYDPVFVPEGY
                     DVTFAEMPLVEKNKISHRAIAAGKLGEFLRKNMNNAASR"
     gene            complement(58818..59306)
                     /locus_tag="PKI35_01100"
     CDS             complement(58818..59306)
                     /locus_tag="PKI35_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNX43002.1"
                     /translation="MKGGEMNVTILMIGKTNDCYLKEGISLFTGRLKHYLTVDWVEIP
                     DLRERKNLTPQQIKSKEAEILIKKLPEKCTIVLLDENGTGYTSVGFADFIQKQVNTGI
                     KDLVFIIGGAFGVSDEIKKKADFALSLSRMTFTHQMIRLLFAEQLYRAMTIIRNEPYH
                     NG"
     gene            59417..59821
                     /locus_tag="PKI35_01105"
     CDS             59417..59821
                     /locus_tag="PKI35_01105"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HNX43003.1"
                     /translation="MKKIFLIMAGMLFSLSALQVQAEPPLAGILENVAAAIRSGNSRE
                     LAKYFSSSVEVSLPEKDGSFSKAQAEMVMKDFFAKNPPVSFTVNQQGASSGGSQFMIG
                     SYKCGSKVFRTYVLFKPVNGQLFIQQIEFETD"
     gene            59868..60707
                     /gene="nadC"
                     /locus_tag="PKI35_01110"
     CDS             59868..60707
                     /gene="nadC"
                     /locus_tag="PKI35_01110"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNX43004.1"
                     /translation="MTIHQIISEALREDIGSGDHTSLSTIPGNASGKARLLVKEDGIL
                     AGVDVALEVFRQVDPDIRVDVFIQDGNPVKKGDIVFEVSGRSISLLTAERTVLNFMQR
                     MSGIATQTHKMVSKLEGLHTRILDTRKTTPVLRELEKMAVRIGGGCNHRFGLYDMILI
                     KDNHVDFAGGIANAIDAALKYLDEKKLNLKIEIEVRNLDELQQVLDRGGVDRIMLDNF
                     GYDDLHKAVKMVDGNYETEASGGITGETIRKYAECGVDFVSVGALTHHISSLDLSLKA
                     VKE"
     gene            60708..61685
                     /locus_tag="PKI35_01115"
     CDS             60708..61685
                     /locus_tag="PKI35_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNX43005.1"
                     /translation="MVAKLLNLQQKLVGVQRYSKLKTLQFVRSDFVTTLIRYSRKLIL
                     PGFNRMPLYDVLVFFIKGLQKGTISMRAAAFSYNFFLALFPTILFFFTIIPYIPITDF
                     QDSLLGLMQSFIPEKAFAAVEGTLFDIIKRPHSGLLSFGFLLALYFSTNGIHSLMISF
                     NQTYHAIDTRSWVKQRLVSVLLVMILSILLIVAIALLTVGPVVLDWLVKHDILKGSGY
                     YLILAGKWLVTLALLFFAFSFLYYLAPAAKSRFNFISAGSTLASLLTILLSVGFNYYI
                     NTMAKYNTLYGSIGTLIIVMVWIYFNAMIVLIGFELNVSIQNARMNKGK"
BASE COUNT        16270 a        13355 c        14826 g        17472 t
ORIGIN      
        1 gccggataag tttccccgtt ataatgtctc cggattttgg gatccatcgg aaatagtaac
       61 agcccggtgc gattttataa atattgagga tttcggtttg actttgttgc cgctgttcca
      121 aaatgaccct gcctgtgtta tcaataagct gaaaaacagc aggtaattga tttgttgcag
      181 gtgcagcaat cgtcaaatag tcatttgcag gattagggaa tagtttaatg ctcaaatcat
      241 ctctgcgttc tttcattccg acctccatta aggaatcaca ggcaagcgga gaaccccaga
      301 tgacatctcc ttttttgaaa tatttcaggt cacggtattc tttgccccaa tatgtcggat
      361 cgtcacaatg ataataggga ccgccaagac ctttaaagta taaggtttga taattgcaaa
      421 atccatcaaa agtaacaggc cataggcaac catttccatc atccgaccac atcatcagat
      481 aatcaaaagc cttgtaagga ataccttcag ggcagtattg tgaattacct gacattccgg
      541 taaaaggata tgtccaccag ccagattcag gtatcatcac aggttccagc ggttccgtat
      601 cgaattgagg tgtataagag ggcgaaactg tctgatcaag agtatctttt aaataagagt
      661 aatcttctac tccattattg tttttatggt aggttaggga acaatattca aacgtgtaaa
      721 ggacagagtc accagaagga taatcataac ggctgaggta gcgtgaaaca atggctgttg
      781 aatatgtata tgtcccgcca taattgtaat tctcggaata tcctgctaca tgtaattcat
      841 cacccggctg aaagtcaaat atatccaatg ttttaggaac ctggtagccg gcgttgggat
      901 tagtttttcc gataaaagag taaaacttag gggagtagct taattcatcg aacttcggca
      961 acctgagcaa accataattc attcccagca tgagggactc cccgtttatc gggtttgcaa
     1021 caatctgacc tgaagtatct ttgcagttta acacgatcac tttgacataa tcggccagtt
     1081 cggggaaatc aaccagttca atatgatcta ctgttgcttc aatatattgc aggccattat
     1141 gataattgta aaaacgccat gattctccga ttccagcctg ggaataaatt gtgtaaacat
     1201 catttgagtc ggaaggggag aacggataaa aaataaactg agttttgcca tccggatatt
     1261 caattatttc gtctccaagc caggacgcac cgtttgtagt ccagcaaccg taatctgttt
     1321 gcctgatctg cctcatggca aagtaactgg tttcagtacc ggaaatagcc acagaatcaa
     1381 ttctcgctga aataatctgc atggatgttg aatcgtaata ataatacgtt gcatccgttt
     1441 tcacagcttg gtaatactgg gctgaagtat ttaaggagaa gaagaaaaaa cagaagagag
     1501 cggcattagc tgcaaaataa attcttattt tcatggccta aaaattaaat attaatttga
     1561 aaagaaattc aaagatagtc aaaagccaaa ataattattc tgcaaagccc gaatttttat
     1621 ttttcatgaa gttagattta ttcaaaagaa atttctaatt ttgcacccct gaaaataaaa
     1681 aagctgacga ccaatggcta agaaaagcaa agaagcgcgc gtacaggtca ttcttgaatg
     1741 taccgagcac aaaaccagcg gaatgccggg cacttcgcgt tatattaccg tgaagaacaa
     1801 gaaaaatacc cccgagcgtc ttgaactcaa gaaatacaat ccgatcctca aaaaagttac
     1861 cgttcaccgc gaaattaaat aattgatacc atggcaaaga aagttgttgc aacccttcgt
     1921 acctcttccg gtaaggactt cgcaaaggtg atccggatgg taagatcgcc caaaaccggc
     1981 gcttacacgt ttaaagaaac catcgtagcc aacgaacttg ttaaagattt cctggcaaag
     2041 aaataatcgt ggatttcacg gttccggcga agctttttcc tttgggagaa agctttttcc
     2101 gtttctgaag gattatttac ttttacatcc tttaatcatc gatcaacaac ctgctgaata
     2161 gtattctttt cctcatcagc aggatcgttt caatgaaacc aattcctgaa tatgggcata
     2221 ttttcgtttt ttacccggga aaacaaggaa acgctgaaca agggccttga aaaaacccgg
     2281 accagcgtgt tctcacgact ttcgaaagca cttgtcggaa aatccaaagt tgatgatgag
     2341 gtgcttgata acctggagga aatcctggtt acttcagatg taggtgtaga aaccaccctg
     2401 aagatcatcg acaggataca gcagcgcgtt tctcacgata aataccttgg caccgatgaa
     2461 ttgaacagca tcctgcgcca ggaaatagca gccttgctgg ccgaaaatga taacaataac
     2521 ttcgagggtt ttgattttcc caagcgtgaa actccttttg tgatcctggt tgtgggagtg
     2581 aacggtgtag gaaagactac caccattggt aaactggcgt ataattttcg caaagcaggc
     2641 aaatcggtgt tgctgggagc tgccgatact ttcagggctg ccgctgttga ccaactcgcc
     2701 atttggggcg aaagagccgg cgtgcccgtc attaagcagg caatgggcgc cgatcctgcc
     2761 gctgtcgctt tcgataccgt aaaagccgcc gtgaaccgta acagcgatgt agtgatcatt
     2821 gataccgccg gacggcttca taataaaatc gggctgatga acgaactcgg gaaaatacgc
     2881 agcgtaatgc agaaagtgtt gcccgatgcc ccgcacgaag tgttgctcgt cctcgatgct
     2941 tcgaccgggc aaaacgccat cgaacaggca aaacagttta cagccgtcac tgatgtttcg
     3001 gcgctggccc tcaccaaact cgacggcaca gctaaaggtg gcgtcgtaat cggcatcagc
     3061 gaccagttca aaattccggt ccgctatatc gggcttggtg agaagatgga agacctgcag
     3121 gttttcaata aagacgaatt cgttgattcg ctgttcgcat aagattttaa caggcaacca
     3181 aaaccggagc cttgtggctg tcaaatgaag accggcaaca tccggttccc ggttcaacct
     3241 ttaaacattc ataatctttt ctccccctat gaaaccgcac actatccgcg ttgtaaccct
     3301 gggctgtgct aaaaataccg tcgacagcga aaaactgatg gcccaactga atgcgaataa
     3361 catccgcgtg attgccgacg gcgacaaggg gcgagccgat acggtgatca tcaacacctg
     3421 cgggttcatc aacgatgcca aagaagaatc ggtggatacc atcctggaat ttattgaagc
     3481 caaaaaacag ggtaagatta aggatgtatt cgtgatggga tgcctgtcgg agaggtttaa
     3541 aggcccattg caggctgaaa tccctgaagt ggacgaatat ttcggcgtca acgatcttgg
     3601 atcggtagtc aggagggtag gcgccgagta ccgccgaacg ttgctgggcg agcgtctgct
     3661 taccacgcct aagcattatg cctatttgaa aatagccgaa ggctgtaacc gccgttgttc
     3721 tttctgcgcc attccaaaca tacgggggcg gcatatttca cgtcccattc ctgaaatcct
     3781 tgaagaagca agactgctgg cagccaaagg tgttaaagag ttactgctca tttcacagga
     3841 cctgacctac tatggcatcg atactcacca gaagcaattg ttgcccgaac tggtagaaca
     3901 actttccgac ctgaaactat tctcttggat caggctccat tatcttttcc ccgcaacttt
     3961 tcctgaccgg cttctggaag taatcgcttc acgtcccgat gtttgcaatt acattgacat
     4021 tcctctgcag cacatagaaa gccacttgtt atcctccatg cggagggggg tgacgcgcga
     4081 agaaaccatt gccctgatta aaaaattccg gaaggctttg ccgaatgccg ctatacggac
     4141 ggctttcatc gtgggatatc ccggtgaaac cgaagaagat tttgccgaat tgaaagattt
     4201 tctcatttat tcggagttcg acagggtagg ggtgtttacc tattcgcatg aagaagatac
     4261 gcatgcattc ggcctgaagg atgacgttcc tgaagaagtg aaacagcagc gggcatcgga
     4321 aattatggag atgcagcagg aaatttctat gcataagaac catctgaaga tcggtaaaac
     4381 gtttaaagta ctgatcgaca ggcttgaagg agagttttat gtcggccgta ccgaatttga
     4441 ttccccggag gttgataatg aagtattaat tgaagttgga aataagataa aaatcggcga
     4501 attttacaat gtcaaaatca aacaggccga cgaatttgac ctgtacggtg aaattgcaga
     4561 cttttaaaac tctattgtca tgaaaattaa ttgcttagta attgcattac tgcttctgac
     4621 cgccaatatg gttttttccc agacggaaac cggctcaaac aataaaaccg acgcatccgg
     4681 attgaaacaa ggtacctggg aagaaaatac aacgacaggg gtggaaaaag gtacatacct
     4741 aaacggcaaa aaggagggaa cctgggtgtc gtataccaat aagggtaacc tggcaaaact
     4801 ggaatctttt aaaaacggcc ttcacgaagg aatatccgtt gaaattgacc agcgtggcta
     4861 cctgctcagc gagacgtatt atgaaaacga cctcatcgaa ggcgtcgcaa aacgttatta
     4921 ctacggatca aatccggcat cggtgatcac atatcacaaa ggcctgaaaa atggtcctta
     4981 taaagtgtac tacgaaaatt cggccggaaa aatcatggaa gaatcgaatt atgtaaatga
     5041 catcaaggat ggcatgtcga agtggtacac gatcaagggt gatctgatag cagaatacaa
     5101 ttatgtgatg aactcgctcg aaggcgttca gaagagttac agcgaaggca ataagcttct
     5161 cagtgaacag acgtataaaa acaacctgga aaatggtccc tatcgtgaat tttttgccag
     5221 tggaaaagta aaaatggaag gctcctgtac cgacggtaaa aagaccggcg tttggaagga
     5281 atacaccgag gatggcaaat tgttttcgga aggatcgtat gtgaacgacc tgaaagacgg
     5341 aaaatgggtt gaatacgatg ctaacggtaa aacctcgaaa gtgacgaaat tcgtgaaggg
     5401 agaaattgta aaataaattt tctcctcttg ttatacccat cacaaaattc tgttataaca
     5461 gcgttttgtg attttttttg tactaattca acaaatctga tttccgcaac atgaaagttt
     5521 cgttaaataa tccgtttaaa ggaagggaag ggtataattg tttcggatgc tctcccgata
     5581 atgcgatggg tttgcaaatg cattttgaga tgcatggcga agacgtgcat tgtgaatggc
     5641 agcctaaaca gcactttgaa gggtggcaca acgtcctgca tggcggaatt caaaccacgc
     5701 tgatggatga aatcgccagt tggtgggtat ttgtcaatat gaaaacgacc ggggtaacct
     5761 ccagtatgga agtgaaattt aaaaaaccgg tttataccga taagggacca ataacgctga
     5821 tagcaaaaaa gcatgaagtc agccgcaaca ttgccgtaat ctttgtgcaa atattggatt
     5881 ccggaagaac ctgttgcgcc gaatcatata tacattattt tacatacaat gagcagacgg
     5941 cgcgtgaaag atttaattat ccggggtttg gagagtttgt gaaagagact gactgaaagg
     6001 gtaggtgtac acagaaccgg ccggaatctg actgccgaag gtaaaaggtt gcctgcgaca
     6061 ggcgtggttg tgtaccagtc tgaagattaa ctgaaaatga caggcagtta aaactatttt
     6121 aatagcacct gacgggttaa tgtctggcgt gacgcagcgt aacattctta tttctaattt
     6181 attaaccatt ttattctttc tgttgaaaac taagaaacaa aattcgggcg aactttgatt
     6241 aaacatccta catttgcacc gttatggtga gcatgccggg gcctccggta ttgctttaaa
     6301 agggaatcgg gtgaaaatcc cggacagtcc cgctgctgta agctctttca accctgggat
     6361 tatctttagg ccactgtggc gttccggcgt catgggaagg catcccgaag ggggagcaag
     6421 tcagaagacc tgccattgca aattaattca acgctttcgg ggataaaagc agtgaatatt
     6481 tccgggtcgg gctattccgt attttatcct tattgtaatt gttaacgctt tatatgatgc
     6541 gtaaactgct gattttattc ctgatattta atgctttagc agcctattca cagcaaatgg
     6601 cggattcggt ccatcgcctg gcaacagtgg aagtgattgc cgacagaatt tcttcaaatt
     6661 cggcaggaat gcaggtccgt caaatagatt ctttaaccag gaaactctat tcggcaacta
     6721 ccctttcgaa gctgatatca gaacaatctt cagtctatat acgttcttac gggcctggcg
     6781 gaacagcatc ccttaccttc aggggattgc agacctcgca atcggtggtt ttatgggaag
     6841 gtataaacct gaattctccg acccacggca tgatggatat ttcgatggtt cctgttttta
     6901 tactggatca gatatcgttg cagtatggcg ggggcagcat gttcagcggc agcggcgcca
     6961 tagggggaag cctggtttta tcggggaagg aatgcttcag ccagcctcta aaggtacagg
     7021 caagattggc tgccggcagt ttcgggaata tttcacccgc ggtaaaaatc tccctcggca
     7081 acaggaaatt ctgttattca tcttccctga ttgccaatag tgctgataat agctatccgt
     7141 atattaacct tgatggaaaa cgtaaaacct ttcagcatgc cgcatatcag tcgctgggca
     7201 caatccataa ggcagagtgg aagatatctg aacatcaaac ggtttctgct gccggttggt
     7261 accaggcttc aagccgtgat attcctccca cgatggtgat gtcgaagagc atgcaacacc
     7321 agtccgacag ggctttgcgg atgagtttac aatggaaatg gctgaacgct gaacaccgtg
     7381 tggtttcagg cgtagcgctc acgagggaat acctgcatta caccgatgga atgtcgggaa
     7441 ttgatgccga atatcgcaac cggacttatt cgtcgcatat tgaatataaa tatgtgttga
     7501 cgccattact tgttttcgat gcgggaattt cggggcgttt actcgttgct gaaacgatct
     7561 attacaatgg aattaaaaac cggcccgaag cagcaggcta tgcttcggtg ataaagagct
     7621 ttccttcggc aggatggaaa gtggctgcaa accttcggca ggatgccgtg gaaggttaca
     7681 gggtgccttt ttgcccttca ttaggagcag aaggacgact ggcaggccgg ttggatgcca
     7741 ggcttaatgt atcgcgcaac ttcagggtgc cgacattgaa tgaccgcttt tggatgcctg
     7801 gcggaaaccc tgatctgaaa ccggaatcat ccttaaatgc cgaagccggg cttgcataca
     7861 gatccggcga aacaaagagt ccgctttcct ggaaggttga attgtcaggc tattccgtat
     7921 tggtcgatga ccttatcctc tggataccct cgggcgggag tacactctgg tcgcctcaaa
     7981 atatcgagga agtatggagc aggggtgttg aggcgtcagg ctcattgagg ctgactgaaa
     8041 aaaaactaaa aagcgaacta tcggtgaatt atacttattc gccctcgacg gttacctgca
     8101 cggctactgc tctcgcggaa aaaaatggta agcagttgat atatactccg ttgcatacca
     8161 taaaaagcaa tttcatgatg caatttcatg attacacggt acaactgcaa aatacttttg
     8221 aaagtcgccg gtttacccag gaagataacc ggaaatggct cccggcccac acgttgctga
     8281 acatctcagc cggcagggtt ctgaagatat ggaagaaaga ctttacaatc cagctcgata
     8341 tcaacaacct cctgaattct tcctatcagg ctgttcagta ttacccagct ccggggatat
     8401 cgttcacggg aagcattact gccgaattct aaaaataacc taatacaacg ttgcaacatg
     8461 aaaaaattaa aactgttatc atggctgact ctttctatcg tgctgtttat cagcgcctgc
     8521 cataaagatg atcccgaaaa cctgaaggga caattttcca atgggatctt tattgtaaac
     8581 gaaggcatat tccagacagg cgtcggaact atctctttta tagaccggtc gacaaacatg
     8641 atcagccagg atatatttgc gcaggttaac ggccggcccc tgggcagcgt ggcacaatcc
     8701 atgacgatca gcaacgggaa agcctatatc gtggtcaaca atgcttcggt tgtggaagta
     8761 gtggatgcgg cgacctttga atccgagggt ataatcggcg gactggtatc gcccaggtat
     8821 tttatggctg tcagcaacag caaaggttat gttacggatt gggccgggaa tgtcgacggg
     8881 gatgtcgccg tagttgacct ggcttcaaat acggtaacaa agactatccc agttaagagc
     8941 gggccggaac agatgctcat tgccaattcc ctggttttcg tgctgaattc gggaggatgg
     9001 agcaccgaca gtaccgtcag cgttattgat cctgcttccg atgaggtgtt gagcacgata
     9061 caggttaatc atcgtcccac tggtattgtt aaagataaat atggcaaagt atgggtgatg
     9121 tgttccggca tgggatttaa cggctatccg caggcgggcg attctaaagg gcatctgatc
     9181 aggatcgacc ctgttaccct gcaggtggat ttcgattatg tctttaaatc gtcatcggag
     9241 catccggaaa aacttgtcat caataaaaat ggcgacaggt tgtttttcct tttcaaaggt
     9301 gcgatcaata gttttgatgt ggataatacc actgaagatc cggtaattct ggcgatgaaa
     9361 gataatcttt acgctttggg atatgatgcc tcaagcgatt atatctatgc ttccgatccg
     9421 cttgattatc agcaaaatgg ttgggtgtac cggtttaaaa gcgctgatgg ggcgttgatc
     9481 gattcaatcc gggcaggcat aattcccggt aatttttgtt ttgggaacta acaggtaatc
     9541 cttagatttg ccctgttatt gcttaagtat gccgttattt tctgaaaacg acctgttggc
     9601 cgaatgcgtg ttcaccttta cccggagcag tggtccgggt ggccagaatg aaaacaaggt
     9661 atcgacaaaa gttgagcttt ggttcgatat tagttcttca cgggtgctga ctacggatca
     9721 gaaaaagatg attatcagca ggctgaaaaa taatgcgact tctatccggt tgacttcggg
     9781 tcgcgaacgt tcgcaaatgg caaacctgaa actggtctcg aaacgatttg tcgagaaaat
     9841 cgggaaattg ctggaggttg ttcctgaaag aattgccaca agaccgccag ccgcctcaaa
     9901 agaaaaacgg ctgagcaata agcgcctgct ttcggagaaa aaagcaggaa gacaaagcaa
     9961 atctgccgga atccatgagg attaagaacg gatataatga ggcatttccg ggggaaggat
    10021 aagcgagatt tccacgaggc cgcttacaac gccatttgag aaccaggccg actgccagat
    10081 cagtttcctg gttccctgct tttcaatagt atatacgttg gttccgccgg tttcgagcaa
    10141 acctcggatt ttcgcagcag aactttcggc gtgacaattg aaaagactcc ggccgatcag
    10201 gtcagtaccg ccatatttcg cgaaggtcaa caatgcttta tcgttcatga aaagaatgtt
    10261 ggcgttttta tcggtaacgg tgatggaagc gggcaattcc tttgcccagt cgggaatgat
    10321 agtatcaagc gttgagtggt tcatgcggta aggaagaaat ttgaacaaaa ataggatgat
    10381 ttgctcagaa tcgttgaaaa tatttatctt tgcacccgca aaacggctcc atagctcaac
    10441 tgaatagagc atctgactac ggatcagaag gttgcaggtt tgaatcctgc tggagtcact
    10501 acaacgaaaa ggctttcata cttattcagt atgaaagtct tttttttctc cgaaatcgtg
    10561 taaaatggca gccatgccgt tgcctgggct gatagaaagg ggctgaaaag cttacgcacg
    10621 attgatgaac atgccggatg ctaacgactt tgccgttgta agtatcagat atactataat
    10681 ttaacgaaaa atttaaaaaa tcaaatattt agtttattaa ttataagaaa agacataaat
    10741 ttgtattaac cgtttcccgg taaaaagaat atttcttccc gaagggtacc tactccattt
    10801 aaaattgtct gctgacattt tatatgcctc gccggtgcag tcctgccaga attcagataa
    10861 aagcagaaca ggaaatagcc acaggtttga tgatccccgt aaggaacacg atgaaagaag
    10921 catgcagtat tttgagaaac aataaaactg atgaaaagga aaatatttcg ataattatca
    10981 ataaatcaat caattaatca aacaaacaaa aacaacatga aaaaaattta ctcattgatt
    11041 gttctggcat gccttaccat ggctgtttca ggacaaacgt acctgttgga agatttcagc
    11101 gggaatgtga tgccgccagc aggatggtcc attgacgggc tcaactcaca atggtcaatt
    11161 tcagcctcaa acaatgccgg aggaacagct cctgaagcag cgttcactta caaaaatgtt
    11221 gtcacaacca cccgcctcat ttcaccgcct gtcgacatga ccggtcagac ttcggttact
    11281 ctgatgttca atcatcgcta cgcattctat gataatcctg cacctgcata cggcgtcgct
    11341 acgcggtcgg caggcggtgc atggactagc atctgggaac atactcctac cgcaagtgta
    11401 ggtccggaaa ccgtcatcct tactatttca aattcggaca tgggacattc cgattttcaa
    11461 ttttgttttt accttaacgg gaacatgtat aacctggata actattggct tgatgacatc
    11521 atgctatttc aacccttgtc gctcgatgca tcgcttaaat caataagtct tcctccttat
    11581 gtggtagcag gcgccagcaa tactctctca ggatctatca tgaattacgg atccacgaat
    11641 atcaattcct ttgatatagc ttacaggatc gacggcggcg cagcacaggt ttcgtcggta
    11701 acaggattga atcttgcctt aggggaatcg tacgaattta cccatagtgt acccattgtt
    11761 ctgaacactg ccggaatata taatcaggta gaagtgaaca ttcagaatgt gaatggcgga
    11821 aacgacctta atcagaataa tgatacgctg aaggctaatg tcggggtttt ggaatatgcc
    11881 ccggtcagga aagtattttg cgaagaagcg accggaacat ggtgcggata ttgtgtaaga
    11941 ggcatatgtt tcatggatta tatggccgaa acgtatcctg aaacctggat cggagtggcc
    12001 gtacacaatg gcgaccccat ggtgaataca gcctgggaca acgaaattcc caatatcatc
    12061 cccagtttcc ccggttaccc tagcggaacg atcgaccgtg ccggttccaa ttactgggat
    12121 ccttcgcaat ttgaagaggg ctaccagaag cggatcgcac aacctacacc ggttaccgtt
    12181 gaaatcaaca attgtgcatg ggacccggca accaggaata taagctttga tctccagtcg
    12241 gaatttctcc tcggaacgga tgacgaaatg agatttgccg ctgtcatcat ggaagacagt
    12301 attcacggaa cagatgacga ttatgcacag gcaaactatt atgctggggg ctcccttggt
    12361 gcgatgtgcg gatttgagaa cctcaccaat ccgattcctg ctgccgatat gtattataac
    12421 cacgtcggac gtgagatcct ggattcccct tacggtactg ccggcagcat tcccgcacct
    12481 attaccagcg gctcctcatt cacctatcat tacacctata acattccggc tgactggtca
    12541 ttcgaacatc ttcatttcgc cgggctggtt atcaacaata ccaccggtga aataatgaat
    12601 gccaataacg ttgttaactg gctgggtact aatgaaccca ccagggttac ttcgctgaat
    12661 gtatatccga atccggcttc aaataatgca aaagtcgttt tcgatctttc gaacggacag
    12721 cagactggcc tgaggatact tgatattacc gggcgtcagg ttgcatcctt cacgcaaagg
    12781 acctatccgg ccggtacaaa taccataaat cttgattgtt cagaccttgc taacggactg
    12841 tacattgtgg aacttaccgt gggcaatacg gtgtttacca aaaaattgtc agtacagaaa
    12901 taaagaagca ggaaaattgt gaataacgaa gaagccgttt ctcaatgaaa cggcttcttc
    12961 tgtttttaac ggaccggaag tcctgtgtat aacttatagg ttggaatctt tttctgagcg
    13021 ggaaaccgga atcttccgtc tttattattc gttggggctg agatattgca aagggattga
    13081 tggtctgtca gaataaaaac cggaatggca gagggtaatc tcaccaccat tccggtttta
    13141 attggttcac tagtctgatt attcagtctg agcgacagtg gaggcaagtt cggccctttt
    13201 gcgttttgag agtcttacct gaacctcata tacgatacat gccgtgacga aataaacgcc
    13261 ggtttgtatc cacatggcca tgatctcttt cttacattca aatactgtag ccccgagggt
    13321 ttccattttg ataaatccga agatggcgtt ggatgacggg aagagttccc tgaacatcaa
    13381 ccaaaacggg ctaaagttgg acaaaggcca gatgattcct gacaggaaga aaagggggaa
    13441 tgacgtaaac aggaaaagca tcatggggct ttcccttgtt ttgaagagta ccgacaaggt
    13501 aaggccaagg aagatgcacg ataacaggaa tggcgtcatc aatgcggcca gttctccgaa
    13561 actccccatg cggcagtaac cgaaaatttt gggaactata ccgatcaggt agacgcaaag
    13621 cagtgcataa atggtgaagt aggccgccga cttgccgaac accatcctga aagtgcccat
    13681 tttatcttta tccataggta caagcgtgcc caacgcatgg cgttcgcggg cagtaccggc
    13741 tgctatcccg atcgcaagga tcagcgtttg ctggatgatc agcatcaggg ctacaggtat
    13801 ggcatatgtt gcgaatccgc ctactgcgtt ggcaatagaa ttgtcgtcag gcagtatcgg
    13861 atcaaccacg gcgccggcct gattcaaatc caatcccttt tgcatcagat tattgatctg
    13921 tatctgcttg ccttcctcca gcgaaacaaa attggctccc atcatcagcg ttttataata
    13981 caggaagaac tccatatcag cataggctga gatgtttgtt tgccggttat tggcgatatc
    14041 ttcgctgaag ctttcaggta taacgataat cccccttatt ttgcgctgtt taaactgttg
    14101 tacggcatct tccatgctta tgcaactgcc ttcaactttt agttcctggg ttgcatccaa
    14161 ctgtcgcata aaattcctgc tttcggaagt ttgtgaaagg tcaacaacgg taacgggcat
    14221 atcggaaacg atctcaggat aatatacaag cgaatacagt atcgggtaca caacaggcgc
    14281 agctattata attatgacca ctcctacatc cgttaatact acctggtatt cccgcagcca
    14341 gattttcagg gtgttcataa acccttcttt cattcgccgg gttatttttt gaagatagtt
    14401 tttcattttg attctatgtt ttctgtgaac atcaggactt gtgacattga aggtcagatt
    14461 gcctgaatgt ctgttttatg gatatcttca ataaaattct gatagatgag tgctgatttg
    14521 agccggttca atacgatcag aggcagcaaa aggaacagca acagatagat gattgatctg
    14581 gcggagtata tcggttcaaa acccgctaat atctggctct ggaatatgtg catataatgc
    14641 cgcacaggga aaccgtatga ccatatctgg aatatcggcg acatcgattc ggtagggaag
    14701 gagaacccgc aaagcgataa tgccagtatg gcatagaaag cagccaggac aagcgcgtgc
    14761 ctcatcatgg gtaaaagtcc gatgcagaag atgccaatgg cctggtatga aagcacaaac
    14821 aggaatgagg agagtataat ccagccaacc ccgccattca ttggtacggc cataacctta
    14881 tacagcatga aattctggaa catcatgagg ataaggaaaa tgattgtata gggcaggagt
    14941 ttgccggtaa gcgccacaac gatggatttg tccgacaatt tcaaccactc gcgtgaagtc
    15001 cgctccttga tctctatacc gatactgtaa acggtcatca tcaggataaa gatctgcatc
    15061 atgatcggaa gaatgattgt cgtgacatac accgtataat tggcccttgg attgaacaac
    15121 aggtgcatat tcagttggat cggctgtacc tgggccatga tttcctgctc tgactggccc
    15181 atagcctcgc gtttctgaat atttattccg gccgataagg tatgagtgat taatgcaaag
    15241 tcgttcctca gcagtccgcc ggcgataaac aatccgttgt ggtaatagta actgacaacc
    15301 ggctggcgat tggccatgat gtctgcctga agattttgag gcaggacaat atacccatag
    15361 attgaacctt tcctgagttc ggacatggcc tctttttctg tctgaaggct catcacaaca
    15421 tcactggcag gcgtcgcatc aatattgcgg gccatcttac gggatgtcga agtttgatcg
    15481 agatcgacga tggcgatggg cattttggcc ggaacgcctt ctttcatgag agagcagaag
    15541 aagagggtcg tcagtaccgg gaagataagg acggccagga tatagagcgt ccctgatgac
    15601 atgcggtcta tctcacgctt tgcaacggcc cagatgcttt taattaatga tttcattgtg
    15661 aattgattga tggttgaatg ttggatttac cggtatcagg attttttccc tgaccgggta
    15721 tcatataggc tatcaacatc aattctttgc atcaaattgc ttgtaatcaa ccaatatgct
    15781 catgccagga cggagtccgt caactttttc aaccggttcg gcgtgtacct caaaggtttt
    15841 aacatcgaaa ccaccgttgt tttttgtcgg tttgaaggtg gcataactgg ccatggcttt
    15901 tatataatta actttgaggt tgatgcactt gttgcccagg gccggaacag ttccggtgaa
    15961 ccggcttccc attttaatgg tcggaagcag gtcttcacgt atgttaaaaa ctacccagat
    16021 atcgttcagg tcaacaatgc tcatgatcgg ggcgcctgag cctaccagtt ctccctgttt
    16081 tgggaagata tccgaaattt caccatcaat gggcgattgg agcatggttt ccgacaggta
    16141 ggattttact tccgaaacgg ctcctttggc gcggtttacc aacgcggcag ccgccagttt
    16201 atcttcctgt tcggcgccgt ttaaagccat atcatactgc gattttgcag cttttgaagt
    16261 agcgatggct gcctggtagt tggcttcggc ttcgtcgcgt ttctgggcag gaaccacgcc
    16321 ttcattaaac atattctgaa ccctgtcgaa ggtcttcttt gccaatgata cgcctacttc
    16381 agctttctgc cataactcgt atgcgccggc aatctgttcg gcacgtgcgc ctttttttgc
    16441 tttcatgctt tgggcagcgg cggcatcttc ggcggaggta gcctgtaaaa gcttggcatt
    16501 cagttcgggg ctgctgagga agacaagggt atctcccttt ttcacttcag tgccttccga
    16561 aacattaaat ttctcaatac ggccgggaac tttaccggat acgcggactt cggtggcttc
    16621 ggcttctccc tgcaatataa cgggctcggg ctttagaatg atccatccga ttatgctgac
    16681 tacagcgatt accagaagca aggttaagaa tgcaacgagc atgcctgaat gtcttttatt
    16741 cattgttttc attgtgattg atgttattgg ttatgaattc gtttctgttt tttgatggtt
    16801 atttgatatt ggagagggta aggtctccgg tagctttgta gcactccaga accgattggc
    16861 gctgagcaaa tattgcctga aggtattcaa gctccgaatt ctgcatttcg gtttcggcag
    16921 ccagcctttc ggtgatgttc agcatgccct cgttatatga tttctgagcc aggtcaaggg
    16981 ccatacgggc agcgcgaagt tgtttttcct tgaattcgac ctgtgaaaca gatgccgaat
    17041 aatttgttat ggctttcgat tggttgagct gcaataattc ggttgcggaa ttttttgcat
    17101 tctcggcttt ctgaagatca agctgggcct gacgaacctt ggaagaacca gcggaacggt
    17161 cgaatacatc ccatttaaat ccgacgccaa ccaggaatgt agggccaaaa ttcaggttgt
    17221 tgattttggt ttctaccttc atgggatctg tcatatcaac atcagcatta aaaatattat
    17281 cgtacctggc tgatgctatg gcctggactt taggcagata attcgatttt tctgatttta
    17341 ccaggaattt ctgggcttcc acgcctgccg aaagagcctt taattcgggc ctgttgttaa
    17401 tatcggaatt tgttttggcg tatagcagcg gctcaagctc cgggtttatg ttttcgaagc
    17461 tttcggccgg ttttccggta agttgcgaca gcttttgatg cagcattgtt ttcttgcttt
    17521 cgtactccga catctttgcg ttgaggttgg cctcggccac ggcaattttc agggtatcaa
    17581 acgaggtagc catgccattg ttcagggccg aataggcaaa ctttttctct gcgtcaagcc
    17641 gggcagccga ttcatcaagc acttttttcg attggttcag gagcgcaagc tgatcataac
    17701 aggtaattac ttcgccgata acatccgagg tgcatttttc ggtaagcgcg tcctgagctt
    17761 tgattttttg gtccagtgct ttgactgcgt tagggacctt cccgccgctg tataaaagca
    17821 tttttacact ggcatcgatt ccgaaatagt tgccgtacag tttcccggcc actttatcgg
    17881 tgatcgtggg gagttgtatt ccttgttgca tcagcagttg ctgaaggttt gaaatttcgc
    17941 tgctcagttg agccagggtc gggaacatgg cagggaaagc cggggtttgc atgaaagcgt
    18001 ttagttggtc cttgagttgg tacaaacttt caaatccggt tatcttatca atatccgaat
    18061 caagaaagga ataagcaaac atatatttac caccgacttc cactttcggg atataagctg
    18121 accgaacagc tttctgttga actccggtaa tttctttttc aatgttttga ttggcaacat
    18181 tgtaatcctt acttaccgag agggtaatta attccctcaa ttctggagat acgccgatca
    18241 tttgtccgtt taccccgatt gttaaagcag tcagggcaat tgtaagcgtc agattcagga
    18301 gctttgtttt cattttgtgc attgttatca gttttctttc ttttttacgc ggttatattt
    18361 cttgtttttt aagccttttt tggggcagaa tcaggagtga aatggaagta attttaagca
    18421 tttgggtttt ggactaaaat ggtagaatgg tcgattacag tgtaaaaaaa attacctttt
    18481 taaaagtccg taaaataaaa tgctgatcag gttatcgatc cttttctcta tatcctgttc
    18541 attgccttcg acaaacaggg ggatctcaag ccctttcatg gcggtaacga tggcaacggc
    18601 agcgaggttg gtatattcaa tattgaactc cccgttggat acaccttctt tcaggatgtt
    18661 ttcaaacatc atgatttctt cctgatcata tttattacgg atgttgttta caaattcaag
    18721 atggctcagg taatcgtttt tcatgacatc ataaaaattt gccagttttt tgaaagcgtt
    18781 catcctgaca agaatgtatg atttcaattt ttcacgcggg tctgatactt tcgaaacggc
    18841 ttcagagagt tcattcttga gcaattcggc ctctttttca acaacagcct ggaagatgtc
    18901 ttccttgctg ttgaaataat agtaaataga acttttacct ttccgggttg ccagggcaat
    18961 ttcatccatg gtggttttcc tgaaaccaaa ccggctgaat atgtgtcggg ctacattaat
    19021 gatggtatct tttacttcgt ctttgtccag cataacatta gcgatagttg tgtattcttt
    19081 tttctgaggc cgcaaaatta tatcaagaaa taatccgatg caattttttt tcgaattatt
    19141 tagtcgaaaa gtcgaaaaaa aatcagaata agttttaagt aattgataat caatataaaa
    19201 caactgaggc cccgatgaac atagctgaaa tcatacaatg actttagtca agacaaaaaa
    19261 tgccctgctt atacaggcag ggcacatatg tatcaggaca tttcaggatt tatacaggca
    19321 taacctactt tagccctgtg aattgtttcc aggtcatggt tttatatgag ccggatccga
    19381 tgtatttcag gatgggcaac atgtcgctgg cctgcatgta tccctgggta agggtaatag
    19441 ttttaccctt ttcatccagg taaatgtagg tcggatagcc aatcctttgt ttagccagca
    19501 caccgggcag ttgatgcgtg gaacgctgtg cggaaggatt tggattaata taagtatcgt
    19561 cgaggtattt gatcgggttt ttgtcttcgg cattcagttt tatggcaata aagctttcat
    19621 ttacatacct gacaatgttt ttgtccgaaa atgtctcttt atccattcgt ttgcaccatc
    19681 cgcaccattc ggtatatacg tcaaccagga caggcagttt tttctttttt gccatcttca
    19741 tgccttcttc aaagccatac cactttatag ccggttcgga agattttggc ggcgctttct
    19801 gggccatgga acccgatgct gccagaatta gtgcgaaaat aatcaggaat gtacggttca
    19861 tattatgttg taacgaaata ggctgatatt tttatcactc gattgaggaa agtaatcgct
    19921 atatatgccg atcgatttcg cgctgctggt ctttcgattt caaggtttcg cgcttgtcat
    19981 acaatttctt gcccttggcg atggctatcg tcagtttggc aaacccatcc tcgttcacga
    20041 aaagttccac cggtatgatg gtgatgccct tgtcttttaa tttttgctgc agcttcctga
    20101 tctccttttt ggtaagtagc agtttgcggg gacgttttgg ctcatggttc agataactcc
    20161 cgaacttgta ctcggaaata tgcatgttca gcacaaagag ctcgccgtta tcgaaggtac
    20221 aataactgtc ggtgaggtta gctttccctt cgcggatcga ttttatttca gtgccggtca
    20281 atacaagccc tgcagtaaat tgctcctgca aatagtactc aaatcctgct tttttattcc
    20341 ggatgcttat ttttcggttt tcggtcgaca tggcgcaaag gtagcaattg ttttaacggg
    20401 catgatgccc ggatagtata aggcttaaag ctttaaggtt atacctgctg taacgttgat
    20461 accggcctgg ggaaaatatc catccagcac tttatagttc ccttcggaat agtatcggta
    20521 aacccatgca ttggattcat aacgataatt aaaaaggttg ttaatcatta tgttaaagga
    20581 taattcatca catattcgtg gtttcagggt gtaaccggcc tgaaaggagt ttacaaagta
    20641 cggatggagg cttctttctt tagcctgggt gttgtcgata tattgccgcc cgacatattt
    20701 gcctgataac cagaatgata gtttctgtaa aggcgagtaa atgatttcac tgtttgccaa
    20761 cacggcaggg gagaagcaga gatcggtatt atggtgctcg accggtatct gtgtccagtc
    20821 atcccagtta tcaatgtatt cggtgaatga aataatcttg ttccggctta acgtaaccga
    20881 agcaatccat tctagctttt ctgaaagcag ccacttagaa gatatttcca ggcccagccg
    20941 gtaactctca ggaacattga ccattacggc tgaacctaca tcattgatgg ctcctgtaag
    21001 ggcaagctgg tcatggtaat acatgaaata aatgttggac gtgatctcag ccttggatgt
    21061 tttcaggtta tgccctgctt caaggttata taatttttcc cgggtaggtt caggttggtt
    21121 tggatcagca tctgtgaaat tgtcgcggtt gggttcgcga ttggcaattg cagcagaagc
    21181 ataaaaactg ttgctgctgt ttgcctggta ataaataccg gcttttgggt tgtaaaagtc
    21241 aaaatgatgc tgttgcgtta tatcacgcgc atcgtcatct ataccatcaa tcaggtaatc
    21301 aatatgccga tactgcaggt cgaggtacag attgagccgg ttgcctgcct ggtaattaat
    21361 ttttgaatag aaattcaggt ctgatttatc cccgttgttc cggtaccatt cctgatcctt
    21421 cagggcattt ccggcaaatt gagtccagat tacagtcccg taatgattgc cgtcgtaacg
    21481 gttccatgat cctccaaaag tagcctggaa acgttttttg tgataattaa gggaatacgt
    21541 aaatccgtag aaatcattgt ccatatactt tcgcctgatc agatcacact ggttgataag
    21601 gctgtcgggt tcgctgatag tatcgcttcc gataatatag aaaggctgct gcaacctaag
    21661 cggttcaatg ccatagctgc tcagttcgtc gtcatcgcgg aattgttcat agtaaccaaa
    21721 tccttttgta taatgaacgg atcctgccat ataaagcttg ctgctgaact gatgcgccag
    21781 gtgcaactga tagtgggttt gcagatagtt atcggtttca ttgtcgtaaa aacgaagatt
    21841 tcctttgctg tcgtaataca ttcccattcc gttatacctg cggttggtat cgagaatgga
    21901 agaaggaatc ccgtcccatg cctggtaagt tttttcgtta ccggagaacg tggtcatcct
    21961 gattagattt ttctgatcgg tatatgtgcc cgacaggtaa taggattgca ggtcaacgga
    22021 ggcccggtca atgtaaccgt cggatgagag cttcgacagg cggccctcca ggttccagtg
    22081 atttttcatc aggccggtgc cgaaattgag ggtgttttta aaggtgttgt atgaacccgt
    22141 tgacgaattg aatacggcat atggttcagg atcagatgtt tcagtctgaa gattgatact
    22201 tgccccgaat gcggcagctc cgttggtgga ggttcctacg cctctctgta tctggatgga
    22261 attggtcgaa gaggcaatat ccggcagatc gacccagtaa acttcatgcg attcggcctc
    22321 gttgagcggt atcccgttaa tggtaacatt gatgcgcgtc atatcggttc cccggatgcg
    22381 caacgagctg tatcccattc cgttgccggc atcggaactg gtaacaaccg atgttgcgtt
    22441 cccgatgaga aaaggaatat cgcgactcag gttcccctga gcaatctcat tctttgatat
    22501 ggtctggctg gtcacagggt cgtttgtgcc tgcacgtgtc gccaggataa taacctcatc
    22561 ctgaacaaaa acagttggcg aaagcgttat cgggaggtta agggatttat ccaccgtgat
    22621 tttttcttca taatccttgt atccaaggta ggtgaccttg atagtataaa tgcctttttt
    22681 aacattgcgg agaacaaagc ttccggtttg atcggtaatg gctgaattgt atgtcccgga
    22741 gagcatgata tgggcgccgg gcagcgtctc atttccggtt tcgccggtga tttttccgga
    22801 aacggtaaat tggggaaagg ccagtaaggg cataaatgcc agtacgacag agagaaagtg
    22861 tagtttcatt ggttgatttt gttaagttaa accttgaatg aagaatgggg gcaatccgca
    22921 tgaagcggat cattcaaagt ttcccttttt ccctacgccg gtattacccg gatcaggtag
    22981 tatgggtttg atctcagccc gtaatttagg gcacccctat tcagaaaatc tgccgcaaaa
    23041 ttataaaatg catccgtgaa aaagcaaagg aagtttaaat atttacctat gcttaagaat
    23101 atcaattgat taatatcaaa gataggaggc aatatcagac atctctcttt tataatgaaa
    23161 ccggatgacg gcttggggca gatcaataat gccggttttg caatgctcga tgactgacga
    23221 atctgacgtg gcgataagtc ccgaatcaag cattttcagg taatgatcgg gagacgggag
    23281 ggtaatagct gaacctttta tttcatagtg gtttagcagg atgttgattt tttcacaaag
    23341 ttccccgcta tggctgaccg gtttatcgaa aaagaaattt atttcccggg gctgattgga
    23401 ttgcagatac gagaaaatca gcaggatcgc cttgttgaaa agttcgttcc ggaatgcttt
    23461 tccatgaatc tccgatgcgt cgcgtacaaa atgatcgctg ctgatgaaaa caagatttcc
    23521 gttcaggtag cttccgatgg tgatcagcac gttataaccg tcgatgttca acactgaacc
    23581 ggtcactgaa tccgggctaa ccagctttgc cgccctcccg gccgattccc gggctgacga
    23641 aataccccgg tacaacaccg acctttccac agaacttaag gcgtaacggt ctccgattag
    23701 tttcaggatt gccttttgcg ggtaattatg ttcaagtaaa tacagataat cctttgccgc
    23761 atccctgaac tttaacccga tgcccgtgtt tttttctctg gccattctgc atctgttccg
    23821 gaaaatcgag gcgcttattg caccgcacat caaaattagt cattcaactc attttataac
    23881 cggggatggc attatatttc acacgatttc aaggcggatt gatacccccg ggctggaatt
    23941 tgcagaacag tccggcataa ttaaaacagc attccggcaa ttaccggtga ttctatgggc
    24001 gctgcctgaa aatggttaaa tttgtggctg tcaacacgtc ttttatgaaa attctcatta
    24061 tcaatggtcc caaccttaac ctgttagggc ggcgggaacc tgaaatatac ggcggtcaat
    24121 catttgaatc atatctcgaa aggctgaaga atatgtttcc tgaaatcatg atcgagtgtt
    24181 ttcaatcaaa tgttgagggt gaaatcatca ataaattgca ggaagccgga tttacttacc
    24241 agggtattgt gctgaatgcc ggcgggtatt cgcatacttc ggttgctatc ggcgatgcta
    24301 tccgttcaat atcagccccc gtagttgaag ttcatatttc caatattatg gccagggaag
    24361 attaccgcag ccgttcggtt atttcgggcg gtgtgaaagg catcatttca ggattcgggc
    24421 tcgattcgta ccggctggct gttttaagcc tgatataagc gtaaaagaaa caggaacaga
    24481 acacctctgg agatccgttt ctcctgaagg atgagtacga aataaaaaat gttgctgaga
    24541 gtattctatc cggaaaattt ttcctgatcc atttccggga atgatttctt tttccggaag
    24601 tggtaatcaa tcaccaggtt cccattgtaa atcatggata catggttgtg gcttagtttt
    24661 tttctcttgg cgcgaagctt aaagaaattg cggtaaaaat acccatgcgc cctgatgacg
    24721 gcataaaaat cggcaaatcc ggcttcgagg aggaatttta aggctgcgat gccgtcaagg
    24781 gggacccgtg ccaggaagac aggccataat aaatcggacg gcaggttttt ataaagcagg
    24841 gccaggttat tcctgaagtt gagataggtt ttatgtgatg actgcttggg cagtgttccc
    24901 ccgccgacat ggtaaaccac cgaatcgggg cagtacatta ccttgtaacc gccgtttttt
    24961 agcctccagc agaagtcgat ttcttccatg tgggcaaaga agtcttcgtc gagtccgccg
    25021 aattcaagat actcttttgc ccttacaaac atacaagcac cggtagccca gaaaacttct
    25081 gaaggcgtat tgtactgacc gttatccgtt tcgatggtct gaaaaattcg tccgcgacag
    25141 aaaggataac cgtatttatc aatgaaaccg ccggcagcgc ctgcatattc aaatttctcg
    25201 gtgtgatggt aagagcggag tttaggctgg caggcggcga ttttcggatc gctttccata
    25261 aggtcgataa ccggtttgat ccagttttcg gtcacttcaa tatcgctgtt cagcaatacg
    25321 aagtagtcgg cattcacctg cgaaagtgca tcgttatatc ctttggcaaa tccgccattc
    25381 gtgatattct gaatgatcct tatccggggg aaattgttct gcagaaattc aaccgaactg
    25441 tccgtactgg cattgtcggc aacgatcact tcggcaaagc cttcgctgtg ttgtattacg
    25501 cttggtaaaa actgccgcaa gaattcacga ccattccagt tcaatattac gacagctaca
    25561 gttggcaagg cagaattgtt aacgaagtat gaataatgct gttggtttag tcagggcccg
    25621 ggtatgatca ggggctgctt tccgaagcga aattaatcaa atattcgaca aataaatcat
    25681 tattccgtta gttatcaatt ttatatttcc agcggcgatg tgaccagagc caattgtccg
    25741 gattttcaat gatatccttt tccagcaatg ctatatgttt gttggtaatt tcaaagtccc
    25801 gggtaagttt cggcgtatcg gtgatcaatt cgaatgtaac ttcgtaatat cctcttttga
    25861 cccttcggat atgcgaaaac ataaccacat gatcgagcga tcgggctatt ttttcggctc
    25921 cgaggaaaaa tgcagtttgc tggttcagga aaagggtatg atattcaatt tcgctcctgt
    25981 gaggcgtctg gtcggcaagg atggccgtca ggttttgatc gcgccggcgt gataataaaa
    26041 cccggtaagc gcttttaaaa ggcagcagga catctttcat gaaccgggtg cgtagggaag
    26101 tgatgaattc ttccgaaacc ggatcgctga gaggtttcac caaagcgcaa ttgtggtcga
    26161 agccggggca ttgcatcgcc gtggccggtg cataccactc ccagtttccg caatggccgc
    26221 tcaccatgat gacacttcgc ccgagccggc ggtaatgatc aagcagttcg ggatttacga
    26281 ctttcatccg tctttgtaat tcgccggcag tcataccgcg ggttttaaag atttcgacga
    26341 tgatgtcgct caggttccgg taaaatttcc gggcaattgc ctccctctcc tgtgtgcttt
    26401 tttcagggaa tgaattccgg aggttttcct ccacaacatt tctccgatag cggattaccc
    26461 gccggagtat aaaagcaata aaatccgatt tcagatatat aacccagaac ggcagtaagc
    26521 tgaacaaccg gttaatagcc ttgatgatgc caagtttgaa attcatcggt ttatatgaaa
    26581 atcattgagg tgtttaatat taccggggat tgtcccataa gtcccttgac cttcccccgt
    26641 aataatcatc ctccccggtc ctttctatgt cttgaggatt cgatgtgtac ctatcagtat
    26701 ctcttttatg caattcaact tcccaccttg cattatagag ctctccgaga acgtctgaat
    26761 gtagtatgtc aaaatctctt gatgaataat catgagtata gaatacaaat ttccggtttg
    26821 tttgggatcc aattttataa tattgccaga tttcataggg ataggctctt ggttcattgt
    26881 attcctgcac tctggaattg ggcgggccgt attgcaggta tacccttccg cggtcggttt
    26941 catacccttt ttttatcgac gttttaaatt catcgttcac agccagaacc tgaatgtaat
    27001 acttttgcca tgcgcctgaa ggattttcgg gggcctttga ttgccagaat gaaaggaaat
    27061 accgctgcat ggtctcaagg ctggcaatat taagctgtgt attgataaaa agtttttcgg
    27121 aagaggatgc tatcgggaaa cacatcctga tgaattcacg caaggagtct ggatttgtat
    27181 aagctgaaac aaaggagttc tggatatcaa tgcttgacag gtcattaagg tcgtatttga
    27241 gcgaaggatt gctgcgctgg aaaaacatcg aattcctgat tatctcttta ttttccttgt
    27301 ttctggccgc aacaaccagg ttatagttcc ccgacgggag attcgaaata tcgaattcgc
    27361 tgaaaacaac gttgaccggt tttacgtcct cgcgtttcat gcgcaggttg tctttgataa
    27421 ctttacctgt ttcgaacgat tcaatagagg tggccacaat aaatttctca tcagcgccca
    27481 ataatcgggc aagatcgtac atttcggtgt aaaaggtcag tttgtttacc gctgccgggt
    27541 aaaaattgtc gagatagggt atgaaatcgt agccgctttt tgtaagcatt cccggttttt
    27601 caaccctggt aaacgattcg atgagttgta ttcccgatat ggcagcctgt tcattgggat
    27661 aattaagttc gaattgatcg gtcaccagga atggtttcat ctccctgttt ttatcggaga
    27721 tggagatgtc aagatcatat ttcccgttgg gcagggcgac tcgctgctgg tcgaggaagc
    27781 tgaaattaat tacggttgta tcgtccactt cagggctgaa taattcatat ttcctgaagt
    27841 cgcggatgga atcgttttgc ttgaaaagca tggtcaccag gatggatccc tgaaatttcc
    27901 cattatcctt atgcacatat tgtacacttt tgcctagtac cgagaggtaa gtttcgatat
    27961 acgggccgtg ttccggtgaa cggaaagtgg tgtatgaaaa gaacgccctt aggtttccgg
    28021 gattcggcat cgccgctacc gagaggaaca ggaaagtgcc tgcaacgaga agctttttca
    28081 tgcttttggg tgtaaatgta cagttgcaaa gttagatata atgctgggta aatacaatcg
    28141 ggcaaatgtt atattttata aggggtatgg ctgatgacgc gacgaatatt gccgcacatt
    28201 tttacattca aataaatttg ataattttgc agcaccggag agatggcaga gtggtcgatc
    28261 gcggcggtct tgaaaaccgt tgacctgaaa gggtccgggg gttcgaatcc ctctctctcc
    28321 gccaaagccg gcctggaact attcccggcc ggctttttta attattccgt caggtaattt
    28381 catgcaattt ccatatcgaa ggaacctgtt tgccctgtac atcatcaagg ttgccaaatg
    28441 gatgaacctg gtgatgcctg ttatcgtgct gttctataaa tcgaacggga tgggcatggc
    28501 cgaaattttt cagttgcagg cagtctattc cctgaccctg atgtttttcg aaattccaac
    28561 cggatatttt gctgataaag ccggacggaa gaccagtatg atatgcggct catttctggg
    28621 cgttgcaggg tatttgatat acagcacttc ctttggtttc tggaattttg tcatggcgga
    28681 agtggcgctt ggccttgggc agagcctggt atcgggggcc gattcggcca tgctgtatga
    28741 ttctttggtt gccgggcgga tagccgataa atatacccgg tatgaagggc gtattaactc
    28801 aacaggcaac tttgccgaag ctctggccgg cattgcaggc ggattgcttg cagcaggttc
    28861 gttgcgaacg cctttctatg ttcaggctac cgttgccgcg ttggcaatac cggcgtcttt
    28921 gtgcctggtt gagcctcccg cagttggcgc taagattaaa ccgacactga aaaatgcttt
    28981 tgcgatcatc aggaaagcat tgcacagcga ccggaaactg aaatggaaca ccttgttttc
    29041 agccgttacc ggagtttcta cgctaacaat ggcctggttt gctcagccgt ttttcatcca
    29101 agccgggcta agcgtatcat ggtttggaat tatctgggct gtattgaacg tgacggcagg
    29161 gatagcttca gcctgtgcat ggaaacttga aaaacggttt ggcccttccg gaaccgtgac
    29221 tgtgttctcg ctggtcctcg tttccggtta tctcgggata tccttccttc cgtccgccat
    29281 gggattttta ctgttgctca tgttctattt tgcccgggga gtagccacgc caacacttcg
    29341 taattatgtg aacctgatca cggtttccga agagagggca accgtacttt ccgtcagaaa
    29401 ttttgttatc aggatgatgt ttgcaatact gggaccggtt ttcggtcgga ttacggataa
    29461 atacagcctc ccggttgcca tggcggcagc aggcgtactt ttaggcacca tgatggggat
    29521 aaccttatat tacttcctga aataccgtac ataccaggcg gattgaaacc tatgccggcc
    29581 atattttttc agaagtgttg gctattgaaa aatagtttct atttttgcag ccccaaacaa
    29641 ttggaaagat gccagagtgg tcgaatgggg cggtctcgaa aaccgttgac ccctttcccg
    29701 gggtcccagg gttcgaatcc ctgtctttcc gccaccagtt taatgcctcg caatgcgggg
    29761 cttttttttg gtcgggggta tacctcattg aaatttttca gcatcatttc ggtaaagttt
    29821 ataatttcgt tttggttcta taagtttctg aaatgatgaa ttttatacca atcggggtag
    29881 gatgccattc gggttataaa gccgatgaat atccggaata ctttatcctg gaggaaaaga
    29941 gatttgaaat cctggagatc tccgaccggt ggtatcaggc ggagaacatt cccggatatc
    30001 cggtttcgga ttattttgag gtcaggacac aatatggcgt atatcttatc cggcatgaac
    30061 cagccggcga caactggtac ctgtgcatat cggataaagg ataatagggt aaaggaaata
    30121 agtgcctgtg cggggaagca acggcaaggc tgatttcaca atacttccat tttaaaattt
    30181 ctgctttagg ggggcatcct ttttctagat aggaaaaact ttgttctgct tcattttcag
    30241 atgcctaaaa taaatacagc cggttttttc tgcagttcag gaatctggct tttccagcgg
    30301 tgtaccggca tggtgcggat gaactcgtcc tgcagcgtaa tgttggcagc gatgcaaagc
    30361 cgggtatcgg gatggcaggt ttcgatgatg gtgtgaaaca ttgccatgtt acggtaaggc
    30421 gtttcgatga aaagctgggg ggatccggtc tgtcgaacct cgcgttcgat caacttaagg
    30481 gatttgatcc gttcgggtgt ttttaccggc aggtatccgt ggaaagtgaa gtgctgtccg
    30541 ctgaatccgg aagccattaa agccagcatg agcgaagaag gccctgtgag cggtatcacc
    30601 ctgatgcctt tctgatgggc caaggacacc agtttatgtc cggggtcagc gacacacggc
    30661 aatccggctt cgctcatcag gccgatattc tttccgccgg cagccggttc aaggtaagcg
    30721 tcaatttctt gatccgaagt atgttcgttc agcaggaaaa aggtaatatc gtcgataagc
    30781 ccggtataac cagccttttt cagaaagcgt cttgccgtgc gtatttcttc cacgacaaaa
    30841 acattgatta ggttcaaaat caggatattg ccggcaggga gacaggtttc ggcagaaaca
    30901 tccccgagcg tggccggaaa caggaataag gagccttttt cagtttgtgg atccatgttt
    30961 attgccaggg aagtttgttc aggtcaacat tgccgccggt gagaattatg ccgatgcgtt
    31021 tgccggcata ccgggtttta ttcttcagca aagcagccag cggagtagct gccgatggtt
    31081 ctatgatcag tttcatccgt tcccaaacca gtttcatggc ttcagcaata ttgaaatcgc
    31141 ttacggtatg gatatccgtg acgaatttcc tgataatcgg gaaagtcagc gagcccagcg
    31201 aggtgcgcag cccgtcggca atggtatccg gattaaccga cggaaccagt tcgcccttgt
    31261 aaaaagactg ataggcgtcg tcggcgcctg aaggttcccc ggcaattact ttgattcctt
    31321 ttccgaagta actggccgac agggcggtgc cgctcagaag tccgccgccg ccaaccggtg
    31381 taataatata atcgagattg ccggcgtctt cgattagttc cagggctgcc gttgcctggc
    31441 cggctataat cctcaggtcg ttatagggat gtatttcggt ggcaaaggtt tgtgccagca
    31501 cctggttcag ggtttcttcc cgggcggcaa gggttggctc gcaaaaagtg atttctccgc
    31561 cataggattg aacggcggct atctttactt ttgatgagtt gcccggcata actatatatg
    31621 cgcggattcc cctgttccgg gcagccatcg acagggcggc ggcatggttt cccgatgaat
    31681 gtgtggcaac cccacgtttt atatcctcac ccggaaggct cattacggca ttggtggcgc
    31741 cgcggacttt aaaagcgccc gctttctgga ggttctcgca tttgaagaac aactcggcgc
    31801 cgcaaatcct gttgatggtt tctgacgtga tcaccggagt gcggtgaatg tagggacgaa
    31861 tgcgatcagc agctccgagg atttcttccc ttgaaggaaa attaacagtt gcggtcatcg
    31921 gtttcttttt gatagttcat ttataatagc gtcggtagcc tggtcaagca aagaataggt
    31981 agtttcaaaa tcagattgcc ggccgtaata aggatccgga acatgtctgt tattgccatg
    32041 gcctgcagcg ttcatgatgt aatccacctt tttacgatcg gattccgaac gtgcataacg
    32101 aattacattc ctgtaatggc tctggtccat gacatagata aagtcgaaaa ggtcgaaatc
    32161 tgcctgccgg aataatctca tggtatggtt tgcgatgtct atcccatgct tcttcatttc
    32221 ataaatagcc ctgtcgtcgg gaggatcgtt caggtgaaac ggctcaaaac cgcaggaatc
    32281 gacaactaca ccgaggccgg cctgttttga tttttctctc atcaaacctt ccgccatagg
    32341 cgaccggcag atattgccgg tacataccat aagaattttt atcataatat ccgggtaaag
    32401 aggttttgag ctatttggca aatgcagttc cggaatgcac agcgacttcc cggcaggtta
    32461 accggtccgg cttcaaaaat agcatcaatt tgtttacgaa aagtttgctg tggagaatat
    32521 tcggtttgta ccggcaaatt taatacgggg cagaagcttc ttttctatcg taataaaaca
    32581 agaagctcct gattgccagg agcttcaaac ggactggttg tgggttctgg ttaaaatttg
    32641 atccgttttt ctatttcttc gacgtaaagc ctgaattgtt tgtcggtttc gatcaggttg
    32701 tttactgtgc ggcaggcgtg gagcacggtt gcatgatcct tattgccgca tacaagtccg
    32761 atagcagcca gcgatgattt ggtatgtttc ttcgaaaaat acatggccag ttgacgcgcc
    32821 tgtacgatgt cgcgtttgcg tgttttcgag ttgatggaat caacctgaat gccgaaataa
    32881 tcgcatacga ttttctgaat gaagtctata gaaatctcac gggccgtgtt ctttacgaat
    32941 ttgtcaatca tttgtttggc cagttcgatg gtaatcgcct ttttattcag tgacgattgc
    33001 gccatgaggg agataagggc cccttcgagt tcgcggacat tggttgtgat gctgtaggcg
    33061 agatattcaa gcacatcgtc gggcatgtcg atgccatctt tgtagatgcg ctttctcagg
    33121 atggcaatac gggtttcaag gtcgggaacc tggaggtcag cggccagccc ccacttgaaa
    33181 cgtgaaagga gcctgggctc aagtcctttt agttctaccg gggctttatc gctggtaatg
    33241 acgagttgtt ttcctttttg atgtaaatga ttgaaaatgt ggaagaaaac atcctgcgtt
    33301 ttttctttgc cggagagcca ctggatatca tcaacgatca gtacatcaat catttggtaa
    33361 aaatggagga aatcgttctg gttgttgttc ctgacagaat caatgaattg ctgcataaat
    33421 tgctctgtgg caacatagag gacggttttt tcggggaagt tgttcttgac ctgtaatccg
    33481 atggcatgtg acagatgggt ttttccaagc ccgacggcgc tgtaaatgaa taatggatta
    33541 aaagctgttt tacccggatt gcaggctact gcatagccgg ctgaacgggc caggcggtta
    33601 cagtcgcctt caataaagtt gtcgaatgaa taggattcgt tcagttgaga gtacacctgg
    33661 atttttttca ggccgggaat gacaaaggga ttgggtatct cttttgcgtt accgtttttc
    33721 aggtctattg gcatggctac cgcaggatta ttcgtagctt tccggttact ggcaggaact
    33781 ttaatcgtat aaggatcact ggtatatgaa ccttccatca caatactgta ttccaatcgt
    33841 ccggttgggc cgagttcatt gcgaatggtc tttttgagca gggttatgta atgctcttcg
    33901 agccactcgt aaaaaaactg gctgggaacc tgtatggtga ggatattgtt ttcgagcttt
    33961 aatggtttta taggtgcaaa ccatgtttta aaactttgat aattcaggtt gtccctgatt
    34021 actttcagac aattatccca aacttcgctg tgcagttctt tcattactac tattcgtgat
    34081 taagttacat cggtttcccg gatatccggc tacttggtta atgatttaaa gatcaggatt
    34141 ataatatatt atgtcaaatt cagcatacga ggagcgtatt tcgttgttca aaaatcaatt
    34201 tataaaagtg aaaaaaaaaa aattcttact cttgacttta aaataaaatt tatgtataag
    34261 gaaaaacacc caaaacgaac aatgcgttgg caagtaattc gtttaaattg tctttaaata
    34321 gttaattgat aagttgttca gacgatcgaa gcggcccagc aatttcctga gattgttcct
    34381 ccggatggca aactcgataa cacaagtcat gccaaactcg ctcgaaataa ttaacggtcc
    34441 ttcttcttta aggattttca ttatatcatt cataaggtcg tacgggtatt caatccggta
    34501 aacttcttta acagtattgg tgattatctg cgcatgggca atagcatctt ttgcggcggt
    34561 gcgataagca tttatcagcc ccggaacacc cagtttcacg cccccgtaat accggatgac
    34621 cacgataagg atgtttgtga ggtctgccga ttgaattgca ccgtagatag gccgcccggc
    34681 cgtgcccgat ggttcgccat cgtcgttcac tctccatgcc gatttttcat tccccagcac
    34741 ataggcgaaa caatgatgag tggcatcata atacttcttc cggaggatat cgagttgttc
    34801 ttttacttcc gtttcggtga ttaccggtat ggcgtatgca ataaaccggc ttcccttttc
    34861 gcggaataac ccttcagcgg ttttctcaat agaatggtac tgatcgtcga agagcattga
    34921 ctaaaagtta attgtaatcc tgatctgttt ttcattaacc aacggcaaaa atagggaatc
    34981 ccggcaaagg ccggtccgat attccggtca aaaattgaag taaaattttc atcgaactaa
    35041 cgtccaatgc tagtgttggt taagtttgca agaaagcctt ggccgtttta ttttttttca
    35101 ggattcgcga tatttcgtgt acgtttggcg aaaaatttcc ggcttctcta ataccttatt
    35161 taatataatg gacagccact ttggtgaatt tgcggcctta ctgacaaccc tgttctggac
    35221 tataacagca ttgtcgttcg aagttgtggg caaacggatc ggctcgctga acctgaacct
    35281 gttgcgcctt tcggtggcaa cggtattgct cggcgtgttt tcatattttt accgggggct
    35341 gttcctgccg gttgatgcca cccttcataa ctggttatgg ctgattttgt cggggctgat
    35401 aggttttgtg atcggcgatt tccttctttt ccaggcttat attgttatag gtgcaagggt
    35461 ttccatgctg ctgatgaccc tggctccgcc ggctgctgcc ctggccggat ggctgatcct
    35521 tggcgaaagt atgtcgctga agtcattact gggtatgatg gtaaccatcg ccggtatttc
    35581 gctggtaata ttcaacagga ctcccggaac ttccaaatcg tcgaagagcg ccagacttgc
    35641 ccacccgctg aaagggatcc tgtatgccct gggggctgca atagggcagg gtgtaggatt
    35701 ggtgctcagt aaactgggaa tggaaagtta tgaccctttt gctgcttcac agatcagggt
    35761 gatggccgga gttgtgggtt ttgcgctggt gtttaccgtt gccggcagat ggagagcgtt
    35821 gcctgcggct gtaaaagaca gcaagtccat gaagtggctt attgtaggct cgtttttcgg
    35881 tcccttcctg ggagtgtcgt tttcgctgat ggccgttcag aataccaatg caggcatcgc
    35941 acaaaccatt atgtcgctgg ttccggttct gattattccg ccatcgctta ttatttataa
    36001 ggagaaactg cgcgcccgcg aacttgcagg cgccctgatt gccgtggcgg gagtatcatt
    36061 gttctttttg tagaagttca gaacaatgaa gccgttttac ggaggtatgc cgtgatttgc
    36121 cggtaatcgg cgctacacaa ctttggtcgg ctgaaactat ccagcaggtg attatcggca
    36181 tggtcgaccc gtgggtacca ctcgcctata tcctttaaat cgcctgtttt acggataaca
    36241 atatgcaggt gcagatgcgg cactgtttgt cctgcggctt ccttctcctg taatgaccaa
    36301 tcgaaccctt caccatggaa agctgccatc agcaggttgg tggttttttt tgcaaaaacg
    36361 aataaccggg ctgtttcgtc gtcattcaga tctgacaacc ccctgacatg ttttcttggt
    36421 acaataagcg aatgcccggg caggattggc gcaatgttat aaacagccag gaaatcatcg
    36481 tcggcaagga aagcggcttc cgaaaactct ttaacgcaga acggacattc tgtttcccgg
    36541 taaacagact tgctcatttt ccgtaacttc cgaaaagttt ggccagaaag cggtgaaatt
    36601 tatgtactga ttcaacagga tcagtataca tcaggatgca tttgccgctc acgaggttta
    36661 gaccggagtc atggtaaggc tccagtacgt cgttgctttc agcgccctgc tgtaaccata
    36721 tgtgcctgat gccgcgtgaa acggcgtctg ctatcagccc ttttgaaatc tcaggccggg
    36781 tgcatatcac caatccggtt acttcgggcg gaagatcaag aatgctgtga taacatgtat
    36841 caccatcaaa ttccgacagg ttagggttaa caggtatcac tttccgtccc ctttttttca
    36901 gggtgagata catggaatgg ccgaacttgt tccggctcct ggaaacgccg gctattgccc
    36961 ataccggctg cgccagaaat tcgttgatag cgggtaatga attcatactg tgaattattc
    37021 aaatacaatt ttcatctcta cccggcggtt gtgctgacgt ccttcaggtg tggcgttggt
    37081 atcaacggga cggtcggggc cgaaccattc agcacggata catgaagctg gaacgctcct
    37141 gtcctgcagg aatttcttca cggccagggt gcggtttttc gataaggtca tgttcgtttc
    37201 acgttttcct acattatcgg tatgtccgga aagctggagc ttccattcag ggtgctttac
    37261 gagcagttgg gccagttcgt taagcgatgg gtatgatacc cggcggatca ccgacttgcc
    37321 ggtttcgaat tcgaggtttg cgaatgcggt gttgataatt tcctgctctt ctttctttat
    37381 ttcagggcaa ccattatttt gggctacgcc gggaatggta ggacacatgt cgtatttatc
    37441 cgagattccg tctccgtcgg tatcgggaca gccccggaat attgccgttc ccggaatgct
    37501 ggggcattca tcatccttgt cgggaatgcc gtcgttgtcg gtatcggtgt aagggcaacc
    37561 gtggttttca acaggccctt ttattccggg acactggtcg tcttcatctt ttaccccgtc
    37621 atcgtcgctg tcgataaacg ggcagccatg gttttcgcgt gtgccgaaca cggtggggca
    37681 gttgtcttcc ttatcggcga ttccgtcgct atcctggtcc ggacaaccgt ttaattcagc
    37741 caggcctgcg ttgaaagggc aatcgtctct tgaatcgata atgccgtcgc cgtcgctgtc
    37801 gggacatccg ttaaactgtg gcaatccttt cacatcgggg caatcgtcct tcgaatcgat
    37861 gatgctgtcg ccatccctgt cggggcagcc ggaaaattct tttagtccgg gttcgttggg
    37921 gcataagtct tcagcgtcgg tcaccccgtc gagatcggca tcgggacagc cggctagttc
    37981 agcaaggccc ggtacgtcgg ggcaattgtc tttcctgtcg gataccttat ctttatcgcg
    38041 gtcgtgtgct tttttgtaac ggataccgaa tttcaaggct acacttgcat tgataccgta
    38101 aatgtccttt ttgccggcga tgatgctgaa aaggttgttt gaacctatcc acagcggccc
    38161 gaaacgtaaa ccggctccaa cattgagctg tttatagctg ttgtactgaa acggcagata
    38221 gaggccgaaa aacctgcctt catatcgggg aaccacgttc caggccgtga gataatgaac
    38281 cttgttgaca tcatttgaac cgcggtttaa ggcaatatag ggagatacat tcaagtataa
    38341 gccttttgcc gctcttacat cagcctgcag gctgatggcg gtcggaaggt tcatcctgta
    38401 tatgtcggac attgagttgt cgaattcaaa gttcagcgag tccttcaggg tgtttaagaa
    38461 gccgctgtat cctttcccga aatcctgatc ctggtaattc caattggcaa tatcggcagt
    38521 aaagtcgttg ctgagtgcgc ctcttttgta tttaacgctg ccgatatcaa ggatgctgag
    38581 cccgattctg aacaggtact tgttctgatc gcgctgccac aggttcgttt ctccgtcaag
    38641 gtcgtattta aatgattccc agtcaggccg gtattcataa acgaagccaa ggtcaaagcc
    38701 aagcgatgga ttggctgcaa ctttgtaatt caggttctcg tagttaaagc cattatcggc
    38761 aaacaggtta tcaaaattat cggaagcgcc gtaattaacc ttggtatgga agatggaaac
    38821 agaatccctc tcaaggaaat tgtagctgaa ctctttggca aatccgtaaa aggagcctac
    38881 gccttgtaaa agcttaacgg tagcgccggc tttcaggaaa tgttcacctt cgtcgagaat
    38941 aacgcgggaa taggtaaagc caaattccgt ccaaaagttt ccctgaatat ttacattggc
    39001 atccctgaga tcctgtttca ggtagtcctc ataattatca ctttcgtata ttatttttgc
    39061 cagttcatgg ctcatattat caagattaat aattcccctg gtgccaaaag tcaacgcaat
    39121 agaggccttg tttgaaacgc tcgccataaa actcatggtt tcggcctgaa agtttatgat
    39181 catgctttta tcatcactgg aagtattttc gctgaggaaa tcctctttgt caaaatcttc
    39241 agcgtcccaa tccgtgctga atttttttgg atttaacaga tagtaaggat ccaacccgat
    39301 atagttatta ttaaatgttc cttcgacgcc tgcaattgtc agatcgaact tgtaccggct
    39361 gtcggcaata gaagccggct gaaggtaaac gccggtgact cctgcataat tgctcgaggc
    39421 aaacggaagg taacgctgtg aataaccgtg agttatgcct gtaaggaggc agatagccag
    39481 gaggaaggtg atttttttca tacaatcagt ttcggattaa ccaaatatac aaaaagcatt
    39541 ttcctgctac cggtccagtg aaaaaatatt ttaattgtca gaacgtttgt taaaaaccgg
    39601 ctgtacctgg tcaggaattg ctggtgcagg gttgttaccg gaatgttatt tcgataattt
    39661 tgccgggttc agtatagaaa aggaatagta tgaaaaattc tattttattg gtagtgttct
    39721 tattagcagg aattaaaggc ttttcacagg taaagtccgg ctggcgcgat ggaggggaga
    39781 tgatcttttc gtggggcaac atcaatcagg gaaatttaga tgccgataat gttccccggt
    39841 tttccctgat cctgaatgag cagcttcagt atcatgtgga ttatggtcag cataccggat
    39901 tttatacggg attgggaatc aggaatgtgg gctttatcca taaattcggc gatacgctga
    39961 aaataaaaca gcgggtttat tccatcggcg tgccgtttgc cctgaaattg gggaacatgg
    40021 ataaaaatgt attttttgcc gtgggcgccg aaatggaatt attcttccat tttaaggaaa
    40081 agacttttct ccatggagaa aaaatgaaaa caggagagtg gttcagcgac cggacggaac
    40141 tgtttcatcc ttccgtcttt acggaactcg atctgggaaa aggtttctat atccgttacc
    40201 gctattatct gaccgatttt ctgaagcaaa aatcagggtc attcccgggc gggattcact
    40261 taccatacaa caactccgga acactgatgt acatctcggt cgggactatg atttccaaaa
    40321 agaaagccag ggaacaactg ccggtgatca ggaaagcgag caatacttat gcttcggctt
    40381 atctcccggg atcatcatcg gcgaaacaat taaaataagg tatgagcgat cccgaaaaca
    40441 gactgatgac cgttggtgac ctggctaaaa cccttcgggc catgctggag ccggtacatg
    40501 gcagggaaga aagcggggca atggtttaca ggctttttga acattatttg cacctgaatc
    40561 gtgcatctgt tgttttaagc cgtcagatgt ttccgggcaa acggcaacag aagaaattgc
    40621 tgcaagcggc ccggcaactg gctgacaaca ttcctttgca atatataacg ggcgaagcct
    40681 ttttcgacgg tttgaccttc agggtaaacc ggcatgtttt aattccgcgt cccgaaacgg
    40741 aagaaatggt gtccctggca gccgggattt ttgccggacg aaggccccat tccatactgg
    40801 atatcggtac cggcagcggg tgcattgccg tttcactgaa gaagcatttt cccgaaagcc
    40861 gggtaatagc tgtggatgtt tcaaaaatgg cgctcagggt agctcagcgg aatgccagcg
    40921 ataacggctt gcaggtcgaa ttcctgcatg gagatattct gaagcctcac ggctttcgta
    40981 atatgcccgt ctttgacctg atcatcagca acccgccata cgtcacggag tcggagaaaa
    41041 gcctgatgcg ggccaatgtg cttgattatg agcccggcaa agccttgttt gtgccggata
    41101 aggatccttt gctattctat aatgctgttg cccggtttgc caggcggttt ttggccggaa
    41161 acggaatgat cctgtttgag attaacgaaa atttcgggaa ggctaccgtt gatctgttaa
    41221 aatctctggg ttacagccgg gcagaagtca ggcgcgattt caggggaaag gacagatttg
    41281 tgatcgcgca gtaagatgcc cgctggcaat tcctggtgtt gcaattcatc cacctgactg
    41341 gtcaacgctg taagtgataa tccggagaaa gagtgatccg gattccatcc tggaaatttg
    41401 acggatcaat accatgaaag cattacagcg atgcttcaga taaggaattc attaagccgg
    41461 gcgggcattc cgggcctgaa atcagttgaa acaagaggcg ttgacgaatg gcatgccgga
    41521 aaaaagttat aggaacataa aaagacatta gagaaaaaga ctgatatttg tacgcgacaa
    41581 atgagaatac acccgtaata aactaattac taaaatacta acaacaaaca agatgacaga
    41641 aaaggttcaa aaactcatga aagacgtgcc ggcatatcgc tggacagctc ttgtactgct
    41701 ggcaagtgcc atgtttttcg gatatatctt tatggatata ttatcaccct tacaggaatt
    41761 gttgcaaaca tcgcggggat ggactccgga tgcttacggg aggtacgctg gttcggaaac
    41821 atttttgaac gtttttgtat ttttcctcat attcgccgga attatcctgg ataaaatggg
    41881 tgttcgtttt accgccattt tatcgggatt ggtgatgtta ctgggcgcat caatcaacta
    41941 ttatgctgta agcgatgctt ttacaggcag cggggccgaa aatgccatga accactttat
    42001 gaatcttccg ctgaaatggt ggaatattac ccctttcttt agcggcatgc ctgcttccgc
    42061 aaaaatgtcg gccataggct tcatgttttt cggatgcgga gttgagatgg ctggtatcac
    42121 cgtttccaga ggaatcgtca aatggtttaa aggaaaagaa ttggcactgg caatgggagt
    42181 agaaatggct attgcccgta tcggggtcgc cgttgtattc atctgttcgc cgattgtggc
    42241 ccttcagata aaacctataa atgcttcacg tccgattgcc tttgccgtat tgctgttagc
    42301 tatcggattg atttccttta tcgtttattc cttcctggat aaaaaattgg agaatcagat
    42361 gggcgagagc ggtgaagaaa aagacgaccc gttcaaaatc agcgatattg gcaagctgct
    42421 ctcaagcaag gtcttctggc aggttgcatt actctgtgtg ctttattatt ctgcaatttt
    42481 ccccttccag aaatatgcca acaacatgtt gcaatgtaac ctcggatata ctgccgacca
    42541 ggcaggccga gtgttctttg tatttccgct gggtgcggct gccatcaccc cgttccttgg
    42601 taatttcctc gacagaaaag gcaaaggggc ttcaatgctg atgctgggag ccattctgat
    42661 gattgtttgc cacctggtat ttgccctggc gcttccggct accaaaggta cttctgttgc
    42721 cccggtgatt gcttatgctt cgattatcct cctcggtatt tccttctcat tggttcctgc
    42781 cgctttatgg cccagcgttc ccaagcttgt tgaaaaccgc tatttaggtt cggcatacgc
    42841 actgatattc tggatccaga acataggcct ttacgctttc cccatcatta tcggaaccgc
    42901 attaaggtca tcaaatcccg gaattaccaa tcctatggaa tacaactaca ctgtcccgat
    42961 gctcattttc gcttccttag gcgtcctggc tttattcctt ggaatctggc ttaaagctga
    43021 agacaagaag aaaggatacg ggcttgaact gccgaacatc aaataggact aatcttaatc
    43081 gattctatta tcgcgtttgg acggaggtca gaaccttcgt ccattcgttt tttataagga
    43141 gaaggattat attccgggtc aaaagttaac tttgcaggca cttaaagaag actaatgaaa
    43201 tcaaaactca ttttactggc agttcttata ctgttatcgc aaatcgctct gccttccggc
    43261 tttcagaaag gtgaaaccag aaataaacca aaacacccga aaaatatcat cctgatgata
    43321 ggcgacggca tgggaacagc ccaactgtat gcaggaatta ccgcgttacg agataatacc
    43381 aacattgagc gctgtcccgt ttcgggcttt gttaaaacgc tttcagccag taattacatt
    43441 accgattcag ccgctggggc taccgcttat gcaattggct gcaagactac caatggtttt
    43501 ctgagcgaaa cgcccgacag cattccaaaa ccaacaatcc tggaaatggc tgaaaaaaaa
    43561 gggcttgcta cagggctggt cgccacatcg gcaatcactc atgccacccc ggctgccttt
    43621 atagcacacg taagcagccg tggtagttac gaacaaattg cggctcagtt cctgaacacc
    43681 gatatcgatg ttttcattgg tggcggatgg aaatatttta caaaaagggc cgacggcagg
    43741 aacctggccg atagccttct gaaacacaat tacgttattg ccaggagcct tgaggaggtt
    43801 gagaaagcat ccgggccgaa actggccgga ttgttaagcg atgaacacat gccgcggtat
    43861 tccgaaggga ggggcgatat gctgccttta tcaacagaaa aggcgattaa tatcctttcc
    43921 ggaaataaaa aaggcttttt catgatggtg gaaggttccc aaattgactg gggcggtcat
    43981 gccaacgatc tgaattatat cgttgaagaa gtggtggatt ttgacaaagc catcgggaaa
    44041 gcgctcgatt ttgccgcgaa agacggtgag acgctggtta tcattaccgc agaccacgaa
    44101 accggcggaa tggccattac cggaggcgat atgtcactag gaaaggttga agcaaaattc
    44161 acaaccggtg atcattcggc tgtaatggtc ccgctgtttg cattcgggcc cggttcggaa
    44221 cgcttttccg gcattcagga taatacggac gtttttaaaa aatgtgtaga gctgctccaa
    44281 ttatcggacg gtaaatagaa caaatgcaac actgctatca ttctgccaga ggatgaaaat
    44341 actcatcgta acagcaacat ggaaggaatc agaaccgctc aggcaggcgc tgggagattg
    44401 cattcctgtt gaaaatattc tctttcgtta ccggttgaat gataaggtta tcgatctgct
    44461 ggtgaccggt acggggatgg tttcgacggc ttacgcgatg ggacaactta cggggagaca
    44521 aaaatatgac cttgccataa acgcaggcat ctgcggtagc ttcaacagaa atatcaggtt
    44581 gggcgaagtt gtgaatgttg tcgcggattc gtttgccgac atgggcgccg aagataacga
    44641 cagttggctc cctatgtcca aacttggatc tgaaaaccag gatgatctat tgtccggtga
    44701 cggaactttc aacagtaatc ccggcaggtt ttcggtactg ttaaaccact tgcggcctgt
    44761 caatggggct acagtcaata cagttcacgg caatgcggag agcatcaggc gcttttctgt
    44821 ccggacggat gcaggcattg agacaatgga aggcgctgct tttatgtttg ccttgaccgg
    44881 ttctgatacc aaattcattg agttgaggtc cgtatccaac tatgttgaac cccgcaatac
    44941 agataattgg aatatccctc tggctatcgg tgaactcggt gttgaactgg tccggttgct
    45001 taaagattta taaaatctga tcggttagcc tccccggcct gtttttagcc tgcgtgtatc
    45061 agggctgaaa ttgcgaacct gaaatgcttt gcttcatgct ctgttctcat tgcaatggca
    45121 ggtgtgcccg gatcctgtcg tggcaaatat gcattgattt ttaacatgaa aacctgaagt
    45181 aatgaatctc aaccttggtt tttctccctg cccgaacgac accttcatct tcgaagcgct
    45241 ggttcatcaa aaggtagata cggagggcct tacattcgat tacatattgg ctgatgtgga
    45301 ggaattaaac cagaaggctt tccggaagga attggatgtc actaaggtga gttttcatgc
    45361 ttttctttcg ttaacaagcc agtacctttt acttgactca ggggcagcgt tggggtttgg
    45421 cgcgggaccg ctggtgatca gcaggaagga gatgcaactc tccggccttc ggggagcaag
    45481 catagcaata cccggcataa atactacggc taacctgcta ttgtcactcg cagtgcctga
    45541 agcaaagaat aaatccgaaa tgatcttttc ttccatcgaa caggcagttc tggaaggcaa
    45601 atacgatgcc gggctgatca ttcatgaaag ccggtttact tatgaatcga agggactcaa
    45661 aaaagtggcc gatttgggag aatattggga aagccttacc ggaagcccta ttccactggg
    45721 cggaattgtt gtcagccggg aaattccgga cgacatcagg cagaaactca accggtcaat
    45781 ccgcaggagc gtggaatacg ctttcgcaaa ttcttacccc ctgaatgggt ttattgctgg
    45841 caatgcgcag gaaatgagcg aagaagtgat gcgtaaacac attgaactct atgttaatca
    45901 attctcggtc agtcttggca ctgaagggaa gcaagctgta ttgaagctat tcgaaatggc
    45961 tcttaaatca gggttgataa aatcaatccc cggatcaatt tttgcctgaa ttctccctgc
    46021 aatcgttact tttgtaattc taatatttgc atgtgaaaca aaataacctc atcgggattt
    46081 tcttattgca gtttttagca ttcatccttc ctgtttctgg ttatgcccag gttgaaaaag
    46141 gaggtatgcc gcgaagcttt tccttgaagg gtttatcaga tgaaacaggg ataattgaga
    46201 taacccctcc tgatcgcgat tcactgattg ctcaggatta ccgggatgct ttactggata
    46261 agggctatcg catgggcgta gttctgccgc tggaaatttc gatgaataac agcggaaact
    46321 ggtatagcat tcagggaggg agaatttgga aactgtgcat caggagtgct ggcgctcagg
    46381 ctttaactct ttactacgaa cgtttttccc tgcccgaagg tgccgatttc ttcgtttact
    46441 cgaaatccgg acaacatatt gcaggagcct tcacgccaga taacaaccct gagggcaact
    46501 gtttcgcaac tcgcctgata ccgggtgatg agattgtgct tgagtattat gagccttttt
    46561 ctgtttcatc ggtgccggtt atagaactaa acggattgtt atatgcctac cgttctgcat
    46621 tttacggttc ggaaaaaaat ggagatgatt ttggcggttc cggctcctgt gaagtgaatg
    46681 taaactgttc cgaaggtgac aactggaaag accagaaaca aggcgttgtg cgcattctta
    46741 caaggatagg caacaattct ttctggtgta ccggctcatt actgaacaac acaagtcagg
    46801 actattcgcc gcttctgatc acggcaagcc attgtgcctt aaacgaatat gctgccacat
    46861 ttgccaattc gaacgatttg atgcagtgga ttttttattt taattatgaa tcggaaggtt
    46921 gcgaagaccc cgtaaaggaa cctgtcaggc aaacgatggt tggagccgaa aaactggcaa
    46981 catcctattc gtttgataat gaccggttgg gttccgattt ttacctgtta aggctgaccg
    47041 gtacaatccc atcgggttac aatccgtttt acaacggatg ggattgcaat gatgtgccgt
    47101 catcgtcagg cgtttgtatc catcatcccc agggagatat caggaaaatt tccacctata
    47161 ccaaaccggt tgtttcgtcc acctgggaat caatacccgg cacacattgg gaaacttatt
    47221 ggtcggaaac tcaaaacggt tatggcgtca ccgaaggagg atcttccggc tcgccactgt
    47281 tcaacaagtc aggccaggtg ataggaattc tgaccggcgg cggatcgtat tgtaatactc
    47341 cgaatttaag cgattatttc ggtaagattg cgtattcctg ggtgtcaaat ggaacagccg
    47401 acacaaacca gcttaaaccc tggctcgatc ccgttggcac cggcattaaa aaaattgccg
    47461 gatcgtataa ttcaacattt cctattgccc gcttcaatac cgatactata actatcccca
    47521 taggttcatc tgtcacatat tacgaccttt cggtcaataa tcccaccgga tggcactggt
    47581 attttgaggg cggcattcct tcggaatcgt accagaaata tcctccggcc attgtttata
    47641 atacttacgg aacattcgat gtaaaactga cggtcaccaa tgaatatgga agcgattcct
    47701 taataaaaga ggattacctt accgttagcg cccgggtgtt tccaaacccg acaacgggaa
    47761 aggttacatt attactggga ccgggtgaac atcctgaact catcatttca gtttttaacc
    47821 aaaccgggca aaaaattctg gaatatgttc ctggcaatag tacatcctct gccatgtcca
    47881 ttgacctgag cggattgccg gcctcggtct attttgtttc tgttcgcacc ggagaatcgg
    47941 ttcaggtaag ccggatagtt aaaatttaat atcaaaagca atattggatc cggatgaaag
    48001 gacttcttga taacgtaaat atccctccct caattcgtaa tatcgccctg aaagtattgg
    48061 atggaatccg cctgacacaa tcagaagcca ttgaacttta ttccgaaact gaactcggtt
    48121 ttatcggtgc tctggccgat catgtccgaa aacaaaagaa tggcgacctg gtttattaca
    48181 accggaacat ccatcttgaa ccaaccaaca tatgcatcta caattgcaga ttctgttcct
    48241 attcgcagca caacagcgga acttcctggg aatacacggt ggaagaaatg ctgaagaaag
    48301 tccgggaaat gggggatgag atcaccgaag tgcatattgt aggcgccgtg catccggcaa
    48361 gggatattta ttattacggg gacctgctaa ggcaggtacg ggcagaaaag ccccaagccc
    48421 atattaaggc cttcacggct attgaaatag attacatggc ccggaaagag gatctgagcc
    48481 tggaagaagg gcttcaatac ctcaaagacc aggggttaaa ttccattcca ggcggcggtg
    48541 ccgaaatatt tgacgctggc atcaggaagc agatctgtga gaaaaagtca acggcagaaa
    48601 cctggctcag cgttcatgaa accgctcata aactaggcat accttcgaat gcgacaatcc
    48661 tttacgggca tattgaaaat atcgggcatc gcatcgacca tcttgaccgt ttacggcaat
    48721 tacaggaccg gacaggcggt ttcaatgctt ttatcccttt aaaatacaga aataaaaaca
    48781 atgaactttc ctggatcgaa gaagcgactg taaccgaaga tatgcgcaac tacgctattt
    48841 cacgcatcta tcttgataat tttccgcata ttaaggcata ctggccgatg attggaaagg
    48901 aaatggcgtc attggctcta tccttcggag ttgatgacct tgacggaaca atcaacgact
    48961 ctaccaaaat ctattccatg gccggcgccg acgataaaag cccgtcgatg agcgtgcagg
    49021 aaatcgccga tttatgcaaa agatcgggcc ggattgctgt tgaaagggat tccttatacc
    49081 gtatactccg gaggagttaa atatacatgt ataattgctt tttgtatttt tattttattt
    49141 cattttaaat atttctgatt acatgtcagg ctaaaatgct gtatttcata ctttacccac
    49201 ctgaaagcat atattttgaa aagttaggaa gtataccttg caagtttatc ttcactgacg
    49261 taactttgca agcttaacct aatactaact caaatattct acatcatgaa acttagaaat
    49321 tttctcgtgg tcgttctcgt tttcggcggc ttgacagcgc ttaatgctca accggttgtt
    49381 aagggtgata aactcctgaa ccttgggatc ggtttaggat ctacgcttta ctccggttca
    49441 tattacacaa gcacattgcc tccggtttct gcatcgctcg aagttgtcgt aaaggatggc
    49501 ctgctcgata acaaagccgc tataggaatt ggcggttatg ccggattagc aggatacaaa
    49561 tatgattatc agggttgggg ctataagtat acgaacataa ttattggtcc aagaggttac
    49621 ctgcattata atttcctcga taagctggat acttataccg gattattggt tggttatgat
    49681 attgtaaaag caactgaatt tggtacagct cccggatgga actatgaagc ttcttcaagc
    49741 gggttaatct ggtcatggtt tgcaggcgga cgttatttct tcaacgataa tcttgccggt
    49801 atggttgaat tgggttatgg tattgcttat ctcaatcttg gcgtgacgtt taaattttag
    49861 ttgaattctc tgaagcgatg cggggaaaag ttgaagtact tttccccgca tcgctattta
    49921 taaatgacat gttttcaggc gatagtttta gaggctatct aaaacttcat attagcctgc
    49981 ggctttcaac aatttcaggc acctcctgat ctcgccgacc cacaggtata ataaaaacgg
    50041 cactaccagc actaccagtt tgttgaaatg ccaggcggtt ttaaaatcaa gatggataaa
    50101 atgcataacg gctcttgtgg tgccgcacgc gaagcattcg cggttaaaac aaatttttga
    50161 aaggcaaaga ttaacggttg atttatcaaa aaaatcagcc ggtaagcata ataaaactac
    50221 tgggctcact aaaagtaagc ccaggtagct tttaatcagg tatttgttat tgaaatccaa
    50281 cttatttcaa tacgtggatc aaggcgtgaa tgattcccgg cacgccgcag agtaacgtca
    50341 ggataacatt tacaataaaa ggcttgccag cttcattata ttttaaataa acagctaacg
    50401 ggggaatgaa gaaagctaaa ataatgagta acaactgatc atcatctgtt gatcccggaa
    50461 gttgtgtggg ctgggcaacg acgttttcaa catccttttg aatggtgacg atttcttttg
    50521 cctgttgttt gccggggaag gctgcatcag ctacctgtac aattccaacc ataaaaaagc
    50581 ctaagaataa cagggcggca attttcgcgt aaattctttt catgagcatt agaaagttaa
    50641 attaaacaat tggttaattt tgctcaaaaa tagtaaaaaa ctcatgaaag tagcgtggtg
    50701 gtgtgtattt tttctgttat tctgtaatgt agcatattca caggcgattg agcttacggg
    50761 agccatcata atcgggaaat cggagatgat gagttacagg atagtatata atattaataa
    50821 taaaaatgag atctccggat attccatagc ggatcttggc ggcgacctcg aaacaaaatc
    50881 caggatttcg ggcgtgttta atccgtcaaa aaagacatta aggtttgcag aaacggccat
    50941 tctgagtaca aaagcgaaat tatcggtcag cgacttttgc ctgatgtccg tcaacggaaa
    51001 atttgaaaaa aaaggcggga aaaatgtatt taccggtagt tttatctcaa aatcgcacaa
    51061 tgataaactg gtgtgcgaat cgggaactct gattctgaca accacaaaag aaatttataa
    51121 ggcagccgcc caagtttcaa aagccattga caaacttcct gcttccgaca gtttaaccag
    51181 ggagatcaag gcaagattaa acccactaaa gggtgtggaa aatgtcaaaa ccctgacttc
    51241 aggcaaatcg gaagagtaca cattaaccga cgacaccgtg caatttgaca tttttgacga
    51301 caaattggaa gacggggata taatttccat cctgaaaaat aatacgacaa ttattcacga
    51361 acttaaaaca acgaacaagg tccagtcttt caggtttgta atcagggcgg atgagaaggc
    51421 cgttacattc accatcgttg ccaataatga aggatcatct cctcccaata ccgtaaaaat
    51481 tgtcatgacc aacggaaaaa caaaagaact gctgatttct caactggcga aaaatcagcg
    51541 ggtgaagctg atattcagga gaaaatgatt gaagtaaccg atgatgacga taccggcctg
    51601 aatgatcttt gctattcccg gcaggctttg cggtatatcc tgttataaag agctcagcaa
    51661 caggacaatc agtaatgaat agagcacaaa agtgacgatt cccagcacaa aacctgcaat
    51721 agccattccc ttttgtcttt tattcaggcc gatagccccg aaaacaatgg caacaggcgc
    51781 cagaaaggga aagccgataa tgcttacaat cccggttacg aggcttacga tcccaaatcc
    51841 gttcatgtct ttcggatctt tcttcccggg cgttttatct gccagatcct gattctgagt
    51901 atttgtggta tcatcggccg gaataattat cccgtgatca ggtgccgtag attttttaat
    51961 ctttctgacg cttgtttcag cacttgtttc aaggccggca atcagttcat cgtcataagc
    52021 ttgatctgat aaattcccat atctatactg ccggatcagg ctatggttac ttccactcac
    52081 gggaggaagt gtcgcaatgg tcttattttc agccaggctg acttgtgaac gctgaagtcc
    52141 tgaatcgcat ttgtcctgga aaaattcctg cgataaattt cctttttttc ggacaggctg
    52201 ctgaatactg tgaccaaatt ttttgggaac caggttggcg acagggtagg aggtaacctt
    52261 cttttctcct gagcatgata tgaatccagc ccctgctgac gccacgatga tagtgaacag
    52321 gatagtcctg acgatccgga ttttcatgat gaaaaaataa attgataaga acggatgaat
    52381 tgttatctgg caaaaacgga gtaattcaat tttataaggg aagacggtta agaatttctt
    52441 tcagtttcat tgctatctgt acgagtatag ttttcccatt ttgcgagaac agaagccata
    52501 tcctcgggca attccgattc gaaatgcatc tcttttcccg tttttgggtg tttgaatccg
    52561 atcgaccggg catgcaatgc ctgacgagga agtatatcga aacaattcct gataaactgt
    52621 ttatatttcg cgaaagttgt gcctttcaga atctggtcgc ctccgtaacg ctcatcgttg
    52681 aacagcgggt gtccgatatg ttgcatgtga gcgcgtatct ggtgggtgcg gcccgtttca
    52741 agggtgcatt cgaccagggt aacataatgg aaacgctcca gaaccttata atgcgtgatg
    52801 gcttctttgc cgtgtttgcc gtcggggaat acatccatca ccaaacggtc gcggaggctt
    52861 cggccgatat tgccttcgat ggttcctgcc tcttccttaa agtcgcccca aaccagggca
    52921 atatatttcc ggtcgacttt atgttcaaaa aagccgcggg ccagaatgga ttgtgccaat
    52981 tcattcttgg ctaccagcaa taatccggaa gtgtctttgt caatgcgatg aaccaggtac
    53041 ggcccttctt cggttacaat gccttcctgc tgaaaacggt acgccagtgc gttcaccagg
    53101 gttccggtat aattaccgtg accaggatgg acgaccatcc cggcttcttt gttgatcacg
    53161 agcaattccg gatcttcgta aaccacattc aacggaatat cttcgggtat aatggttgta
    53221 tcgcgcggcg gatatgccag caggatggaa atggtatcaa gaggcttgac cttgtaattg
    53281 ggcttgacag gaatgtcgtt taccaggatg ttcccggcat gggcagcatt ctgtatttta
    53341 ttcctggagg cattctggat acgtcccatc aggaacttgt cgatacgtaa caacgcctgc
    53401 cctttgtcca cgacaaagcg gaaatgttca tacaggtcat tctcttcagc ctgctggtca
    53461 aggatgtcgc tgtcgtcggc catgcccaaa cgtttgttat taacgggcga taataaatct
    53521 ttgagaagct gtccggttgt tgtcgtggtt cacaagtctg gccaggaaga tgccgttttt
    53581 cagattagcg gcgctcacca caggtctgaa actgttttga taaaccattc ttccggatga
    53641 atcaaataat tctaaatcaa tattatggtc tgttttccac tcctgcggca atgcaatgtt
    53701 gaattcgtca actgtcacgg gatttggaaa aatagtaaat aacgatgatg cggatagttc
    53761 cggatttgaa agcgggttaa caagcccgac aaccggcctc agcatcaacg atcctgcatg
    53821 ctgttcatcg ctcatctgcc aattgccgga aacgttgtaa aaccttttat cgtgtgaatc
    53881 gttataccgg tcgaacccga tattaaggtt gtcaattgaa ctttgttgcc atcctatgta
    53941 aaagagaagg ccgggaaacg atgtttcatc aacaattata ggcgtatcga accagaacgt
    54001 ctgaaaaaca ttcaggctgt cggcatttgc cggaatgata tctttcatct ggccgacgat
    54061 ctgtcccgga gcgccaaggt aatcgttcca caccgtcaga tcgatgtact gattgttggt
    54121 gcctgacagc gtttgattga tgtagagttg aattcctctc agcgtgtcag ccacgttgag
    54181 ttcaaactga acggcatatg atcctgccga accgttgagc ccgacaccgg cttcagcagt
    54241 tccgtcgtcg tatgcataat aattgctgaa cacctgggtg aaacttattg tatcgttttg
    54301 tttgatcagt aaattcgggt ctgtggtcag aaagtgggta gaatgaaaga caaccttctg
    54361 ctgatcgttg gtgccgatgg ggtaaaatga ttccaccggc ggatgggcaa agggagcgta
    54421 agtggagtac ccgtatccgg aataaggata tatggaaaaa ttgcctgcat cgtattcttc
    54481 ataaggctgt tgtgaatcct tgcggatagt gtagccgtac gagatattgt gaggtacgtt
    54541 atcaatgttg gcaatgctga tatccagcgt atccttcatc tcgttgagga aatttttccg
    54601 gtattgccgg tatggcatag cctggtaacg gctgagcatt gaaggcgctt tttcggcaaa
    54661 ggcaatatcc ttcatggctg tatcatattt cgaccggctt gtattcagat aaaccaggtc
    54721 gatgttccac tggtcaccgt tgctttgcca gtcgggaaca tagctgttgg ccaggcttgc
    54781 ataattgcgg aaacggaaac ggaacccttt tttaaagtac ctggcgctgt ccgtaacggg
    54841 tatcattacc tgcttgaagt acccgtggtt ttcaagggca aaagtatcaa cctgaacgcc
    54901 tcccggtgaa gaccaaatga catcccagcg aggggaataa gttgtatcgg tttcggtaat
    54961 cagggtatcg aaattggccg gcgaatggaa ttcgagcagg agtgaatctt ttttggcagg
    55021 catagcgccg cgtccctggg gttgataata aaaactcagg taaacggaat cgctgattgt
    55081 gatggctttc gggaccggtg aaaatattga atcgaggcgg ataggctggg atgtgagttt
    55141 gtcagcttca aaagggtatg atcctgcttc cggataaagg gcccctgttt catcaatggc
    55201 atcaagggtg gctacaccga tagtaagcgg atgccaggca taatcagtat taataaatgc
    55261 atagttgtcg gcccattttt ccggcaacgg ccaaacggtg attctggaaa aatcgtcgta
    55321 aaaaggaagc gtgttgtaga ccgtatcata gttggctgcg cactttaacc ctgcctgaca
    55381 aaatttattt ttaacggtaa tgttatactc aagccctgaa actacttcct gggcaaaagc
    55441 ctggaatgtt gctgaaaggc aaaacaaaag gaatatggtg attttatatc tcatcataaa
    55501 tgtttaataa tcaatggatg cactgtcgtt ttgtatggtg tcgataacga gttgcttgat
    55561 gtaagtgtcg aaatcgaaag attccgatga tctgtatgtg aggctgattg ctgaagagct
    55621 tctgacggta agcccgtata cataggccgg gttttgctga tatacccggg catcaaggct
    55681 gtcgccgtcg atgaattttt cgtcgccaac gagtaatccg agttgttgaa gttccacctg
    55741 ggcttctgca cgggtcttac cgatcaggaa aggaatctgg aaagctcccg atcctgtgcc
    55801 aagcccgacc acgaggtcga tggtagaacc tttgctgata gatgtgccgg ggcttattga
    55861 cgaaccgtta tgcgacatcc ggagcaccat gtttttggca atgtcgggta tcattgaaac
    55921 attgcctaat cttaacccgt atgtttcgag caaggccttg gcctggcgta tcgaaaggtc
    55981 cacaagatca ggcattttta ctttttcggg tacggtagca acaaccgaaa tatagataat
    56041 cctgcctggt tttacgatcg agtttggaag aggatcctgc atggtgatcg ttcctccttt
    56101 ttttgtgagg tcgaagacgg aatcgactac gacataatta aaatcatcgc tcgattcaag
    56161 ctcctgagcc tgggccagcg tgagtcctgt gatcttgggg acggtcacgg tttccccatg
    56221 gcgcgtgtat tgtttcagga tcatcaggat tatccaggca agcacgatgg ttatgagaac
    56281 cgaaagcagg aaatgtttga caaatttccg ggtagtgata aactggataa aattcatggt
    56341 gtattgctgg agcgttcctt gttgggtgtt ttaacagcca tgtgaagtaa taactgccaa
    56401 aaggcctgtt tattgtctgt ttggtgcaaa gataagaatt tatacagccg ccactgttga
    56461 tgatttgtca ttacctttgc ggcgtctgtt aaagctttgc ttggaattac caaagatcat
    56521 gaaatcaaag aaaaacattg ccctgttaat gggcggagat tcaggcgaat ttgaagtttc
    56581 agtggccagt gcagccgtca tcaggagaaa tatagatgaa accctgtaca acgtatatcc
    56641 tgtcctggtt catgcgggcg aatggattta tacgcctgat gaaaaaacag ggattcctgt
    56701 ggataagaac tcattcagcg tgctgaccca caatgaacgg attttttttg actgcgcctt
    56761 tatagccatt cacggaaccc ccggtgaaga tgggaaactg caggggtatc ttgacatggc
    56821 tcatatccct tatacaagtt gtgacctgac aacttcggcc atcaccttta acaagtattt
    56881 cagcaatgac ctggctcttc aatatggtat gaatgttgcc gctacggtaa tgcttcgtcg
    56941 tatggaatcc tgggatacag aatccctgat cagaaaagta ggaatgccat gctttgtgaa
    57001 accaaacaaa gccggatcga gttgtggcgt tacaaaagtt taccatcctg aagatataga
    57061 gaaagccgta aacaaagcct ttgccgagga tgatgaagtg cttgttcaac aattcatcaa
    57121 aggaagggag ctggcctgcg gtgtgttcag gcgtaaagga aatattgttg tattgccggt
    57181 gactgaagta attccgaaaa atgatttttt cgattacgaa gccaagtaca catcaggaat
    57241 gtcggaagaa atcaccccgg catcgttgac cggggaggaa gtggcggaat gcaaccgcat
    57301 tacagcttac ctttatgaca agttcaactg caaaggggta gtgcggttcg attatttcct
    57361 ggataacggc aaattctggt ttctcgaagt gaataccgtc cccggcttat ccgatggcag
    57421 tatagtgcct aaacaggctg tggctgccgg atacagcctg aaggaatttt tcggcatcct
    57481 cattgaagag gctcttgccg gaacaaagta aaggcggcaa ttcccttagc gggatgctgc
    57541 attattcata ttttttctga gaaattcacc caacttgccg gcggcaatgg cccggtggct
    57601 tattttattc ttttcgacca aaggcatttc ggcaaaagtg acatcgtagc cttcgggaac
    57661 gaaaacgggg tcgtacccga acccttcttt tccccgcatt tcttcaatga tcctgccatt
    57721 gatcacccca tcgaaaaagt attgatctcc gccggtcaga aatgcgatta ccgtcctgaa
    57781 tcgtgccgaa cggttggtta cgcctttcaa ttcgttcagc aatttgatcg tgttggcctt
    57841 aaaatcgtga ttgttgcctg cataacgggc tgaatataca cccgggcggc catccagcgc
    57901 atcaacttcc aggccggtat cgtcggcaaa acaatccaga ccaaaatgct ccctgacata
    57961 ctgagctttc tggagcgcat tgccctgaag cgtaccggct gtttcgggga tatcctcatg
    58021 acagcctatg tccgaaagac ctttgataat aaaatgcgga cctgccaggt tcctgatttc
    58081 ttctaattta tgctggttgt tcgtggcaaa tactatttcc ctcatctata gtgttttagg
    58141 gggtgttgtt cgtgattcgt tttgcgtttt gtgttatgat ttttgcattc tgcgttctgc
    58201 gttctgtgtt tcagcgtttc agcgtttcag gttttgttta gttgttagcc gttggccgtt
    58261 agccgttagc ctggcgtctt acccacaacc ttgcacaggc aggtaaactt ttagcgttct
    58321 gcgttttgtg ttttgcgttc tgtgttctgc gttctgcgtt ctgcgtttcg tggaattcgt
    58381 aattgacatt cgtggaattt gtggttcgtt tttcgtgggc agggtttcag tgtttccggt
    58441 tttgtttggc tgttagcttt tagcttttag ctcttggcct ttgacgtttt gagttttgag
    58501 ttttgagttc tgagttttga gttctgcgtt ctgcgtttcg tggaattcgt aattgacatt
    58561 cgtggaattc gtggttcgtg attcgttgtt cgtgttttgc gttttgcgtt ttgcgtttcg
    58621 tggttcgttg ttcgtgggca gggtttcagt gtttccggtt ttgtttggca gttagctttt
    58681 agcttttagc tcttggcctt tgacgttttg agttttgagt tttgagttct gagttctgag
    58741 ttttgagttt tgagttctgc gttctatgtt ctcgtcctcc gtcctccatt ctccatcctc
    58801 cgtcctccgt cctaccctta tccgttatgg taaggttcat tcctgatgat cgtcatggcg
    58861 cggtacaact gttctgcgaa cagcaaccgt atcatctgat gggtaaaagt catccgggaa
    58921 agagagagag cgaaatctgc cttctttttt atttcgtccg atactccgaa agccccgcct
    58981 attataaata caagatcctt aataccggta ttcacctgtt tctgaatgaa atcagcaaac
    59041 ccaaccgagg tataacctgt gccgttttca tcaagcaaaa cgatggtgca tttttccgga
    59101 agctttttta tcaggatttc tgcttctttt gattttatct gttgcggggt taaattttta
    59161 cgctccctca ggtcgggtat ctcaacccaa tccacagtaa gatagtgttt cagccgtccc
    59221 gtaaaaaggg aaatgccttc cttgaggtag cagtcgttag ttttcccgat catcaggatg
    59281 gttacgttca tttcgcctcc tttcatactc ccgaatgcca aagatagtaa caggtaatca
    59341 tttccgggat gacggtatta tatttttcac tactttggtt cgatttttgt aattcaacga
    59401 gcattcagcg atagcaatga aaaagatatt tttaataatg gcaggcatgt tgttttccct
    59461 ttctgcgctg caggttcagg ctgagccgcc tttggcaggc attctcgaaa atgttgccgc
    59521 tgccatccgt tccgggaact ccagggaact agcaaaatat ttttcgagtt cggtggaagt
    59581 ttcccttcct gagaaagacg ggtccttcag caaagctcaa gccgaaatgg tgatgaaaga
    59641 ttttttcgca aagaatccgc cggtttcttt cactgttaat cagcaaggtg cttcaagcgg
    59701 cgggtcccag tttatgatcg gatcctacaa atgtggcagt aaagtgttca gaacctacgt
    59761 gttattcaag cccgtaaacg gccagttgtt tattcaacag attgaattcg agaccgattg
    59821 attttcggag aaggctggtt atctttgcgg caactaaaaa agccgacgtg accatacatc
    59881 aaattatttc agaagcgctg cgcgaagata ttggcagcgg cgatcataca tcactctcca
    59941 ctattcccgg aaatgcttca ggcaaagccc gtttgctggt caaagaagac ggtattcttg
    60001 ccggggtaga tgttgcgctg gaggtattcc gccaggttga tcccgatatc cgggtggatg
    60061 tattcataca ggatggcaat cctgttaaga aaggagatat agtttttgag gtttcgggac
    60121 gttccatttc attgctcaca gccgagcgca ctgtgctgaa ttttatgcag aggatgagcg
    60181 gaattgccac gcaaacgcat aagatggttt caaaactcga ggggcttcac acccggatac
    60241 tcgatacgcg taaaacaacg cccgtattgc gcgaactcga aaaaatggct gtaagaattg
    60301 ggggaggctg caatcacaga ttcgggcttt atgatatgat ccttatcaag gataatcacg
    60361 ttgattttgc cggtggtatt gccaatgcaa tcgatgcagc gcttaaatac cttgatgaaa
    60421 agaagcttaa tctgaaaata gagattgaag tgcgcaacct ggatgaattg cagcaggtcc
    60481 ttgatagggg aggagtggac cgcattatgc tcgataactt cggttatgat gatttgcaca
    60541 aggcagtaaa aatggttgac gggaattatg aaaccgaagc gtcgggcggt atcaccggag
    60601 aaacaatccg gaaatatgcc gaatgcggcg tagacttcgt ttcagtcggc gctctcaccc
    60661 atcacatcag cagccttgat ctgagtttaa aagccgttaa agaatagatg gtggcaaaat
    60721 tattgaatct tcagcagaaa ctggttggtg tgcagcgcta ttcaaagctg aagactctgc
    60781 aatttgtcag aagtgatttc gttacaacgc tgatcaggta ttcccgaaaa ttgattttac
    60841 cgggctttaa caggatgccg ttgtatgatg tcctggtatt cttcatcaaa gggctccaga
    60901 aaggtacgat aagcatgcgg gcggcggctt tttcatataa tttttttcta gccctttttc
    60961 ctactatcct gtttttcttc actattatcc cctatatccc gattaccgat ttccaggatt
    61021 cattgttagg actgatgcag tcgttcatcc ccgaaaaggc ttttgcggcg gttgaaggga
    61081 ccttgtttga tatcatcaaa aggcctcata gcggtttgct ttcctttggc ttcctgctgg
    61141 cactgtattt ttcaacaaac gggattcaca gcctgatgat ctcatttaac cagacctatc
    61201 atgccatcga tacccggtca tgggtaaaac aacgccttgt ttctgtcctg ctggtgatga
    61261 tactttcgat cctcctgatt gttgccatag cccttctgac ggttggtccg gtggttcttg
    61321 actggcttgt gaaacatgat atcctgaaag gatcgggcta ttacctcatt cttgccggga
    61381 agtggttggt tacgctggct ttactgtttt ttgccttctc atttttatat tacctggcgc
    61441 ctgcggcaaa aagccgtttc aattttatat cggcaggctc cacactggct tctcttctga
    61501 ccatcctgtt gtctgtcggt ttcaattatt atatcaacac gatggcgaag tacaatacgt
    61561 tgtatggttc gataggaacg ctcattatcg tgatggtctg gatttatttc aatgcgatga
    61621 tcgtattgat aggttttgaa ctaaatgtaa gtatccagaa tgcgaggatg aataaaggga
    61681 aatagatcca ttgaatatcc ctttttaacc acagagccat ggaggcgctg aggtcaccga
    61741 atttcactta gaaaacatgc aattctctgt gtttcttggt gttccgtagc tccgtggttc
    61801 cttcaactcc cttctccctt ctccgtcctc cgttatccct tctccgttct ccgtccttac
    61861 cgtatcagcg taatggttcc ccattcggca tcctggatat cgtcgcgttt gccggtatcc
    61921 tgg
//
LOCUS       DAOPYV010000005        60922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_354_length_60922_cov_11.570391, whole genome shotgun sequence.
ACCESSION   DAOPYV010000005 DAOPYV010000000
VERSION     DAOPYV010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 60922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60922
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_354_length_60922_cov_11.570391"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..836)
                     /locus_tag="PKI35_01120"
     CDS             complement(81..836)
                     /locus_tag="PKI35_01120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX43006.1"
                     /translation="MQTAERSSVLDPSEQVIYNRCLFAYETSRALIKGNVIELGSGEG
                     YGIRLLAPSADRYLAIDKFEPVLEASGNIEFRQMLLPSLAGIPDDSFDVAITFQVIEH
                     IKNDKEFVSEIHRVLKKGGKLVVSTPNRLMSLTRNPWHVREYTGKQLSALLSGKFQEV
                     TLKGVFGDDKVMAYYDRNKQSVRKIMRFDVLNLQYRMPRWLLKIPYDIMNRMNRRKLL
                     KSNNGIVSGVTVDDFRLKEYNDAAFDIYAIATK"
     gene            1209..2435
                     /locus_tag="PKI35_01125"
     CDS             1209..2435
                     /locus_tag="PKI35_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNX43007.1"
                     /translation="MNPQLLFRNTDTGSKANFSKKNSLKVLILEEFYRNGRKSIPELS
                     KSIQMSTPTITRAIDELMAEDLILEEGIGSSSGGRRPNLYGINPTSRYVMGIDVNRFY
                     VRIGLFDFNNQPAAEIKVFNEGLETQENISAFLRRSVEEYIRESGIDEDKLIGIGIAM
                     PGLIDIHTGITYSYLHDEKPATIMFENLFDHPVFVEHDTRAMALGEQAFGLAKGKSNV
                     LCLNIGSGIGLGMILNGKLYLGHSGFSGELGHIQVDAEGDLCYCGKIGCLETVASGTT
                     MIKNARKKLESGATSLLRTLVDEKLEKINSELILEAAQRGDQFAIGLLSNIGEYLGKG
                     IAVLIHIFNPELIIIGGELTKADNYLIDPIQQNLNKYTISKIRRDAQITTSSLGNNAG
                     LMGTVALVMNKAFSRE"
     gene            2530..5721
                     /locus_tag="PKI35_01130"
     CDS             2530..5721
                     /locus_tag="PKI35_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX43008.1"
                     /translation="MKNRRCIFVMLLICFANLSFAQQKAVNDWENPGLTSVNTEKPHV
                     TMIPFANESQALKNNRAESPFYKSLNGKWKFNFAGNPELAPADFWKNGYNCSGWNDIE
                     VPSTWEVQGYGYPIYVNIRYEFFTDNPNPPMVPHDYNPTGSYITTFTLPENWQSKQVF
                     LHFGAVKSFFYLWINGQYVGLSKDSKTPAEFNITNYLKDGENKIAMQVMRWSDGSYLE
                     CQDMWRMSGINRDVYLFARPDAGIRDFFAAGSLTNNYEDGLLRLTVYFDLKRNAGISD
                     RKLKVSLFDKTTSSPVFSDLRELSEIGGKTDSLYLEKIISQAKQWSAEYPNLYTLVLS
                     LIDNDGNTLESVSNRIGFRTSEIKDGFLLVNGQKIRIKGVNRHEHDPLKGHVISHELM
                     LKDVQLMKQNNINAVRTCHYPDDPYWYELCDEYGLYVIDEANIESHGMGYNLNRTLGN
                     DRRFLAAHLDRTRRMIERDKNHPSVIIWSLGNEAGNGYNFYNTYLLAKSLDKSRPVQY
                     ERAGLEWNTDIYCPMYESVEGILNYASKHQDRPLIQCEYAHAMGNSSGNMADYWDAID
                     SHDQLQGGLIWDWVDQGLLKFDSIGKKFWAYGGDFGPKDVPSDGNFCCNGLVFPDRTP
                     HPALTEIKKVYQYVGFRLANEGVPSIEISNKYAFYDLANTRLVAEVMVDGVSKEKIEI
                     PAPILLPGESTVLPVPLATLFPKNSEVFLNVFLKTDDDRPLMGKGFVLASEQLPLPAS
                     SLTKPAASPVIIKTSSLPPLKTTETNEEITVNGRNFSLSISKTSGAIAKFLYKGQEII
                     VNGPVPNFRRAPTDNDAGNRMFRTCEAWYTDSGKRNAGSVTLDNSNAHFPVITVVYDL
                     PATGSQVIVKYSVTGNADVFVDYSLKGVDPKLPVIPRVGLNMQLNGAFRNVEWYGRGW
                     GENYQDRKSGSFIGIYKGTVDELYVPYVRPQENGYRTDVRWLSVNNGTGCGVLIQGQP
                     LVCFSALPYTYDDMKGFSHGGKHIDQLPDNPFVDVNIDLLQAGVGGDDSWGAWPMEKY
                     LPEAKDYHYSFRMRPYLQKENTPVKLCRQQISF"
     gene            5742..7703
                     /gene="nagB"
                     /locus_tag="PKI35_01135"
     CDS             5742..7703
                     /gene="nagB"
                     /locus_tag="PKI35_01135"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNX43009.1"
                     /translation="MGLSYNVLHRQPIEKEIETDAFVTRFEKMPLRILGSSSEGSLIV
                     ARRIADLIKERSKTGRNAVLGLATGSTPLNIYRELVRMHRDEGLSFSNVTTFNLDEYY
                     PILPTHIESYNHFMHEHLFRHIDIKPENINIPRGDISREQVAGFCREYEQKIAGAGGI
                     DIQILGIGRTGHIGFNEPGSSFTSKTRLVTLDRLTRVDAADAFNGEENVPHFAITMGI
                     GTIIQAREIYLLAWGEGKAEIIQKAVEGPVTDLVPSSFLQNHANTTIILDMGAAAELT
                     RFKTPWLVGSCKWDDRMIRQAVLWLCMKAGKPILKLTERDYNDNGLSELITDHGPFSK
                     ININVFNDLQHTITGWPGGKPGVDDSTRPERALPYPKKVVIFSPHPDDDVISMGGTFA
                     RLVEQHHDVHIGYQTSGSIAVADDDAIRYLDFVQDYEDIYKTECHEGSQKYHEILEFY
                     RRRKKDTIEPKELLELKAAIRRGEARASCRYIGLPEENVHFLNLPFYETGTVKKNPAG
                     EADVIAIMEFLRAIKPHQIYAAGDLSDPHGTHRVCIDIILEALDRMKDDPWIDDCRVW
                     LYRGAWQEWELDKVDMAVPLSPEEVEIKRYAIFKHQSQKDGALFMGSDKREFWQRAEE
                     RNKNTAKLYDKLGMAEYEAIEVFVRYDVK"
     gene            7756..9033
                     /locus_tag="PKI35_01140"
     CDS             7756..9033
                     /locus_tag="PKI35_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HNX43010.1"
                     /translation="MAKDSTLPVSGKTSYIIPITVIGILFFIFGFITWLNGTLIPFLK
                     ISCELNNVQAYFVTLAFYIAYFVMAPPSTVVLHKVGFKNGMAIGLFIIAIGSLVFIPA
                     ALTRTFGLFLTGLFIQGTGLALLQTASNPYVIVIGPRESAAKRVAIMGICNKVAGALS
                     PLILGAVILNQNTTDVVEQLKTITGADRIPMLDLLAHKVILPYIIIAIALALLSIALK
                     FIHLPDVDTDQEEDTASSNGSHKTAWWQFPHLWIGFATLFLYVGAEVIAGDTIILYGL
                     SQGIPLDTARTFTTFTLLSMVLGYIIGIITIPKFISQSKALAISAVLGVLFALAAIFT
                     PGLTSVVFIALLGLANAIMWPAIFPLSIADLGRFTKAGSALLIMGIAGGALIPLGYGK
                     LVEMIGNQQAYWVLLPIYIFIFYFAVKGHKIRK"
     gene            9055..9963
                     /locus_tag="PKI35_01145"
     CDS             9055..9963
                     /locus_tag="PKI35_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNX43011.1"
                     /translation="MKPTLLILAAGMGSRYGSLKQIDPVGPSGETIIDYSVFDALRAG
                     FGKVVFIIRKDIEADFREVFISRLSKHADIEYVFQELDMIPPGYSIPAERTKPWGTGH
                     AILMARNAIHEPFAALNADDFYGAGAFDVMAKYLNSLTPATQTDYAMVGYDLGKTMSK
                     NGAVSRGICRKDDSNWLIEVTERTKITYTGNGIADVQPEKEPLLLHENDVVSMNFWGF
                     TPEYFNQTGRLFEEFLQQNIEKPASEFYIPLAVDTLIKQKQARIKVLQSHDQWFGVTY
                     KEDKPLVVNNIKQLIQSGYYPGKLWK"
     gene            9972..11096
                     /locus_tag="PKI35_01150"
     CDS             9972..11096
                     /locus_tag="PKI35_01150"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNX43012.1"
                     /translation="MEKDIRSIISHFLSAGCFSEAKPLLTGHINDSYLIFTANNRQPV
                     YFLQWINNYIFKDIPGLMQNILEVTRHLEAKQCRGGNAECTVLKLIPCDTGDFFYQDD
                     AGNFWRLYNFIPGTHAYDVVDSPEIAYEGGKAFGKFSSDLADLPASRLKDTIPDFHNM
                     ETRLAKFYQSVRDDKAGRRKGVRPEIAFVEERAAVMLCIPEMIRNGKIPQRITHNDTK
                     FNNILFNEKNHAVCIVDLDTVMPGSILFDFGDAIRTGANCADEDEKDLSKVDLNLPVY
                     EAYTKGFIESTHTSLTREEIGHLSFAARFMTFIIGLRFLTDYLDGDPYFRTRYEDHNL
                     VRARVQFRLVEKMEENAIRMDEIVQKAISEQQKNRVSLPK"
     gene            11105..13426
                     /locus_tag="PKI35_01155"
     CDS             11105..13426
                     /locus_tag="PKI35_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNX43013.1"
                     /translation="MKFKKLCLAIVILLAGFTLTAGNDFSVVPKPMKTEAGKGKFTIK
                     PSTVILTDAGNKEQRLLSKMLVQKIGDAGGPLLRIEDLPAGRIPKNAVVLTFQGAGND
                     IPDEGYTLSVTKKNILIRARKGQGLFYGIQTLFQLLPPSIESHSDGRNAVFAVKSVEI
                     TDMPRYPYRGMHLDVGRHFFPVETIKTYLDIMAMYKMNTFHWHLTDDQGWRIEIKKYP
                     ELTKIGAIRKGTQIGRTDEQDGVPYGGFYTQEEIREIVRYAASKYITVIPEIDMPGHM
                     VAALAAYPQLSCTGGPFEVRTQWGVADDILCAGNEDVFDFVQNVLLEVMDLFPSKYIH
                     IGGDEAPKARWQACPKCQARMKEEGLNNEMELQSYFTKRIERFLNSKGRKLIGWDEIL
                     EGGLAPEATVMSWRGIQGGIDAARQNHDVIMTPVDYCYLDYYQADPATEPLAIGGYTT
                     LKTVYSFEPTPPVLNAEQAKHILGPQGNLWTEYIATPEYLMYMAFPRGIAIAEIGWSQ
                     KADRNWDDFSRRLQNQLDRFDIMGINYSKGSFKTDITTVRDSILNRNLVKITSEAAGY
                     TIHYTTDGNSPDASSPVYSAPFPIDRTSTIKAVLVKDGMVKGTPTECEILVHKAAGKP
                     VTILKPYSFKYPGTGINALTDGLKGGNSFKVGWQGYEGSDMEVVIDLLKEEPVKSIST
                     AFVQDAASWVLFPAEVKYYTSNDGKSWNLADTFVTEPAPTKGKTNHNYTSELKGFSAR
                     YIKVAATNVGKLPDWHENKGQPCWIFADEVIVK"
     gene            13449..>14623
                     /locus_tag="PKI35_01160"
     CDS             13449..>14623
                     /locus_tag="PKI35_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNX43014.1"
                     /translation="MKQFLFLALLLVTTGAYAGHKFPEVRHVVLIGIDGVSAEAFQYS
                     PTPVMDKMARAGSLSLKTRGVMPTVSAPNWATILSGAGPEQHGVTSNNWSLKKPGFEA
                     TVHDEKGYFTSIFTLIRQQRPLANTAMFYDWDWLGTYVNQDLIDTMSYLPGFRAVTDA
                     ASSYIARQKPYFTFVYYGHPDEIGHEKGFNTSEYYQAITEIDGEIGRLIDSVKYAGIY
                     DNTVFIIVSDHGGKGHGHGGESMIELEVPWMISGPGIRQNAVLQEPNDLSNTAPVIAI
                     LLGLQLPVEWTGRPVAEAFLRNKQYNKKNFAGYVSKPMCSVNNGIYLEPQTAALSSTM
                     ENAEIRYTLDGTIPDRKSRKYIEPVILASSVTLTSVAFSENSRSEPAVIKVNVVKGIR
                     "
     assembly_gap    14624..14723
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14724..15238
                     /locus_tag="PKI35_01165"
     CDS             <14724..15238
                     /locus_tag="PKI35_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNX43015.1"
                     /translation="NSRSEPAVIKVNVVKGIRDINLAEAPSSKYPGQGAPGLVDGVRG
                     PDDYFDGAWMGFEEKNIDATIDFGKKRDITSIGIEFLQQPKSWIFLPEVIEYYISPDG
                     VNWELTGDLKPADADGSLQSGPVTLKRNFDLIITRYLRIKVKNIGACPEGHPGAGQKA
                     WLFISEVTVE"
     gene            15287..16261
                     /gene="rfaD"
                     /locus_tag="PKI35_01170"
     CDS             15287..16261
                     /gene="rfaD"
                     /locus_tag="PKI35_01170"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HNX43016.1"
                     /translation="MSYTIVTGAAGFIGSCMVKKLNDNGISNIVVVDDFSNPVKNRNL
                     EDKAFAAKVHRDDFFTWMEEHARETECIYHLGARTDTTEFNREIFDKLNLNYTKNVWR
                     LCSRSGIPLIYASSAATYGAGEYGYTDRHDIVERLKPLNPYGESKNEFDKWMLAQSET
                     PPFWAGMKFFNVYGPNEYHKGRMASVILHSFKQISETGKVRLFRSHKPEYQDGMQLRD
                     FIFVKDVVDILFRMMQKKPEPGLYNLGTGHARAFLHLTRAVFSAMNKPENIEFIDTPL
                     DIRDKYQYFTEACMDKLLKNLGPELKFHSLEEGVTDYVQQYLMKGGYY"
     gene            complement(16393..16737)
                     /locus_tag="PKI35_01175"
     CDS             complement(16393..16737)
                     /locus_tag="PKI35_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNX43017.1"
                     /translation="MALRKEKVGRLYYRIGEVAEIFGVKASLIRYYENEFDIIRPHRN
                     NKGNRLFTQADVDNFHKIFYLVKEKGFTLEGAKSKLKEERRSTGNKEDVIESLRHIKR
                     FLLELRKELSEE"
     gene            complement(16817..17788)
                     /locus_tag="PKI35_01180"
     CDS             complement(16817..17788)
                     /locus_tag="PKI35_01180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNX43018.1"
                     /translation="MVKVRYQFNTKSLKIEEVRVTLKDRLRRLFSALAFGLVFGGIGL
                     IVLYNFFPSPREKVLQREVEQYKLQYKILNDRMDQVQLVLSDLQDRDDNIYRVIFETE
                     PIPSSVRKAGYGGANRYSRLEGYNNSEIITNATKKLDGITSQLYVQSKSYDEVFKLAK
                     SKEKMIASIPAIQPVDISDLRRIGSFFGYRTDPFYKVIKMHQGIDFTASIGTPIYATG
                     DGVVTEAEYNRGGYGNALKISHGYGYDTFYAHMSRFKARKGQKVKRGEVIGYVGNTGK
                     STAPHLHYEVHLRGTPVNPIYYFFNDITSKEYAAMIELSSKPSQTMD"
     gene            18000..20609
                     /gene="alaS"
                     /locus_tag="PKI35_01185"
     CDS             18000..20609
                     /gene="alaS"
                     /locus_tag="PKI35_01185"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNX43019.1"
                     /translation="MTSAEIRRTFLDFFASKQHNIVPSAPMVIKNDPTLMFTNAGMNQ
                     FKDIFLGIAPVKYPRVADSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     KEAIAWAWELLTVVYKIDPSRLYVTVFGGDDHDNLPRDTEAYDIWKKWIAEDRILPGS
                     KKDNFWEMGDSGPCGPCSEIHIDIRNADERLKTPGHELVNKDHPQVIEIWNNVFIQYN
                     RLANGSLVPLPAKHVDTGMGFERLCMVLQGKQSNYDTDIFTPIIGEISRLSGIRYGDS
                     AKSDVAMRVIADHLRAVSFAIADGQLPSNVKAGYVIRRILRRAVRYAYSFLNVRDPFI
                     FRLVPVLEQQMSGQFPELKSQFQLIQRVIHEEETSFLRTLAVGIQKFQQYVQSQESSK
                     VIAGDFAFELYDTYGFPIDLTQLMSRELGWSVDMDDFQVRLDQQKSRSRKDASVETAD
                     WVELIPSTGSEFIGYDTTSAEIKITSYRKVIAKGKEIFQLVFDKTPFYAESGGQVGDT
                     GYIESPDERIEITDTKKEHGLIVHLSETLPENPAAGFHARVDVNKRILSADNHSATHL
                     LHYSLRSILGTHVEQKGSFVSPDYLRFDFSHFQKLTDDETARVERMVNRLIRANISLD
                     EHRSVPMKEAQKMGALAFFGEKYGDAVRVIRFGDSVELCGGTHVKATGQIGLFKITGE
                     SAIAAGVRRIEAITADKAEEFFNTQVGVVEQLKSLVKNPNDPLRAVQQILDQQAELKK
                     QLESLNAEKAGKLKDELARKAEPVNGINFIAAKVDLDSQAIKDLAFALKARVENLFLV
                     LGNESEGKAGLTVMISDNLVSERRLNAGQIIRDLAKNIQGGGGGQPNYATAGGKNPSG
                     LSSAFEQARKIVG"
     gene            complement(20635..21312)
                     /locus_tag="PKI35_01190"
     CDS             complement(20635..21312)
                     /locus_tag="PKI35_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX43020.1"
                     /translation="MNTETIISLSHASVFQKNILILSDVSLCINRGEFVYLIGKTGTG
                     KSSLLKTLYAELPLTEGEGEVAGYNLRELKRKEVPYLRRKLGIVFQDFQLLNDRSVND
                     NLLFIMKATGWKDAEAMRSRMNEVLDKVGLITKGFKMPHQLSGGEQQRVSIARALIND
                     PELILADEPTGNLDPETSEGILQLLFDIRRAGRAVFMATHNYNMIDKFPSRTVRCEAG
                     KVHDSGN"
     gene            complement(21402..21977)
                     /locus_tag="PKI35_01195"
     CDS             complement(21402..21977)
                     /locus_tag="PKI35_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HNX43021.1"
                     /translation="MELLKIVILALIVNNVVLAQFLGICPFLGVSNKVGTSIGMGVAV
                     IFVMTLANLVTYLIQFFILVPLHIEFMQTITFIFIIAFLVQVVEIILKKAAPALYQAL
                     GIYLPLITTNCAVLGIAILAVQKNYNLISSVVYAASIAAGFTLALILMAGIREQLELN
                     GYPKGMKGFPLSLITAGLLSLAFMGFSGLVK"
     gene            complement(22029..22625)
                     /locus_tag="PKI35_01200"
     CDS             complement(22029..22625)
                     /locus_tag="PKI35_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNX43022.1"
                     /translation="MSMLKYLTNGLWKENPTFVLVLGTCPTLAVTTAAINGIGMGAAT
                     TFVLVCSNIMIAGLKNVIPDKVRIAAFIVIIATFVTIVDLVMKAYTPDLYKTLGIYIP
                     LIVVNCIILGRAEAFAQKNKVMPALLDGLGMGIGFTLAITFMGAIREILGNGSVFDIR
                     LISQEARTILIFILPPGAFITFGYLIALMNRIKSKYSI"
     gene            complement(22622..23248)
                     /locus_tag="PKI35_01205"
     CDS             complement(22622..23248)
                     /locus_tag="PKI35_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HNX43023.1"
                     /translation="MSKKLESTLKNMVLCLFLMSVIMAGALGFVYKITKDPIEQADKQ
                     KEIQAVKDVLPPFDNDPTADARDMEGLKLYTATQKGEPVGYAVKTFTEKGFSGHFELM
                     VGFKPDGTINKIVVLNHSETPGLGSKMKEPKFADQFNGMNIGTIPGKQLKVKKDGGTV
                     DAITAATISSRAYCDGVQKAFNVYMNMLNKNQANVDTTSGATSKGGKK"
     gene            complement(23285..24280)
                     /locus_tag="PKI35_01210"
     CDS             complement(23285..24280)
                     /locus_tag="PKI35_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNX43024.1"
                     /translation="MQLLTISGSPHIHGDQNVKKIMWGVVLALVPAMLVSLWYFGLGA
                     LISMAVAVFSCVAVEYLVTKFVLKQEPTINDGSAIITGVLLAFNVPSNIPWWELVIGS
                     IFAIAVVKMSFGGLGKNPFNPALAGRVFMLISFPVDMTSWPLPIQNRFVLVDALTGPT
                     ALGTLREGMKSGETLSQITSQLPDYGNMLLGNIGGSLGEVSAIALLIGGIYMLFRKII
                     SWHIPVSYLLTVFIFTGILWLINPDKYIDPVFHLLAGGLILGAFFMATDMVTSPMTTK
                     GMLIFGVGCGLLTVLIRVWGSYPEGVSFAILIMNAVTPLLNKYIKPKRFGETVKA"
     gene            complement(24285..25616)
                     /gene="rsxC"
                     /locus_tag="PKI35_01215"
     CDS             complement(24285..25616)
                     /gene="rsxC"
                     /locus_tag="PKI35_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNX43025.1"
                     /translation="MLKTFKKGGVHPEENKISAGKAVEVLPPSQKVYIPLGQSLGAPA
                     KPLVDKGSEVKTGQLIAKGESFISANVHSSVSGKVIKIDDITDPSGYRRKAVFIDTVG
                     DEWLETIDRTETIKRDCDLSAQEIIRKINEMGIVGMGGATFPSHVKLMVPEGKKAEIL
                     IINGVECEPYLTADHRLMLEKGEQILVGVTLLMKALGVAKAVIGIENNKPDAISHMQE
                     LAKSFKGVEVVALKVKYPQGAEKQLIKAITGREVPSQKLPIEVGCVVNNVGTAFAVYE
                     AIQKNKPLFERVVTVTGDKLASPSNFMVRIGTPVSELIEKAGGLPAETGKIISGGPMM
                     GKALNSADIPVMKGTSGIVLVPEEKSKRMEMRNCIRCGKCVSACPMGLEPYLLEREVN
                     AGLWEEAENSRIMDCMECGCCLFTCPSGRPLLDYLRLGKNKVGQIIRSRTK"
     gene            complement(25670..26536)
                     /locus_tag="PKI35_01220"
     CDS             complement(25670..26536)
                     /locus_tag="PKI35_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNX43026.1"
                     /translation="MNQAILFAVATLGIIGIASAAILYIVAQKFKVIEDPRIDEVADV
                     LPGANCGGCGFAGCRNLAENIVKAGTIESMRCPVGGNEVMKQVAAIMGLTAEEKEPMI
                     AVLRCNGSRASAPKKVEYDGALNCQFANALYNGESGCPNGCLGLGDCVSSCQFGAMYL
                     DETTGLPVIDEDKCVACGSCVKSCPRRIIELRKKGPKNRRIYVCCMNTEKGAVARKNC
                     EVACIGCGKCVKACNFEAITLDNNLAYINFEKCTLCRKCVEECPTGAIQEINFKPRAP
                     KQSAEKQEEVIA"
     gene            complement(26967..27407)
                     /locus_tag="PKI35_01225"
     CDS             complement(26967..27407)
                     /locus_tag="PKI35_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNX43027.1"
                     /translation="MSDNTKMVQATNLQGLFRTMDVRQFAEAFPDEEHCLQILAEIKW
                     KDGFACRYCGNTRYGKGKTPFARRCTRCKREESATSHTIFHHCRMELPKAFEIAWYVC
                     GKPGISASALSEMLETRHMTCLRFRNRILNCINGHRDLTDPAHE"
     gene            27529..28053
                     /gene="trxA"
                     /locus_tag="PKI35_01230"
     CDS             27529..28053
                     /gene="trxA"
                     /locus_tag="PKI35_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNX43028.1"
                     /translation="MKHLMLTSAIALLLTITSCGNGNTGSGLSEKSPETPGKPEAANT
                     GIPVLSGGEPVVLTKALFLEKVYDFEKNPDNWVYKDNKPCIIDFYADWCRPCRMVAPI
                     MEEFASKYEGKITIYKVDTDKERELAGYFGIRSIPSVLFCPVKGNPQMTQGALPKEDF
                     QKIINEFLLAPASK"
     gene            28091..28456
                     /gene="trxA"
                     /locus_tag="PKI35_01235"
                     /pseudo
     CDS             28091..28456
                     /gene="trxA"
                     /locus_tag="PKI35_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139937.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            28602..29318
                     /locus_tag="PKI35_01240"
     CDS             28602..29318
                     /locus_tag="PKI35_01240"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNX43029.1"
                     /translation="MNVTKQLPQVILPLACLPDIEYFCWLFHASGVLVEIQETYPKQT
                     CRNRYSIETANGVLNLVVPVTKPDGNHTCTRDILLDHKSPWARIHWRTIESAYNKSPY
                     FLYYREAFESIFLAPPKLLVDFNLKVLEACCGILKTDPGIILTEYYRKGYENAVDMRQ
                     LIMHKRKTVHTWSVQEFEPYMQVFSDRHPFFPNLSIIDLIFNEGPSAAHYLYRHCPGN
                     KPVKQPDIQSSHDGCSSVSS"
     gene            complement(29263..31332)
                     /locus_tag="PKI35_01245"
     CDS             complement(29263..31332)
                     /locus_tag="PKI35_01245"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNX43030.1"
                     /translation="MKFRINGSGKYYYEVNKALLFLAALAVLVSIFPRQGKLKSEFQK
                     GKPWQHEELIAPFDFAIRKSESELQQERREILNNAYLYFRFDRDVAESQRERFIQRLS
                     EEWKQKHGNSDQATLNEMQSSGLKIIDTLFSHGIIEMTDDLETKDGDFKIALLQGNVA
                     TIFPLNHFFTVRTANAYISRQLASAPPDNSALTLQLLQESIAHNVLFSEALNREQREQ
                     ALRNISVTRGMVQKGEKIISNGELVTDEKYRVLESYRDEYETRIGRLSDFRMALAGRI
                     ILISVALIVFALFMHTFRRDIFIQNHNIVLLLSLITIMVSATTFIVNRDVSWLNAVPL
                     CLVPIIVRTFYDTRLALFVHIITIIILGFIVPNSFEFLYMQLIAGIITIISVTNLTRR
                     SQFFFTSLWIFCTYSVIYIGMTLLQEASLSGIYRFNFVLFGISALLTLFSYPLIFLFE
                     GVFGMVTDVSLIELSDTHSKLLRELSVKAPGTFQHSMMVSNIAEDAARAVGANTLLTR
                     TGAMYHDIGKMDMPLYFIENQTGTLNPHDELASDESARIIISHVIRGIEKARQYKLPE
                     PIIDFIRTHHGTRYTQYFYSAYKLKGSANPVDESVFIYPGPVPFSKETSILMMADSVE
                     AASRSLKSPDEQKISEIVENIINDQLKNGQFVSSELTLKDISVIKKIIKKKLMSIHHV
                     RIEYPAV"
     gene            31434..32210
                     /locus_tag="PKI35_01250"
     CDS             31434..32210
                     /locus_tag="PKI35_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNX43031.1"
                     /translation="MTGKCPLSLVPLRLEPSDRTEMVSQLLFGETYHIVEELKGWLLV
                     KVLHDGYEGWIDIKQSMPAEYNQAVEPEVKTVHVVAGISDPAFKSGDDKPLILCRGSV
                     LPQFRKGKFMLGGQEYRFDAETSIIPEEPCPEAIVATSLAYLNSPYLWGGRSPFGIDC
                     SGFTQMVLRLCGIPLKRDASLQAQQGEIVNFVNEAKPGDLAFFENAVGNIIHTGILTG
                     DGKIIHASGWVRIDSIDHNGIFNAETGKYTHTLRIIRRMA"
     gene            32414..35584
                     /locus_tag="PKI35_01255"
     CDS             32414..35584
                     /locus_tag="PKI35_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX43032.1"
                     /translation="MKIRKLLLLTFTLFWTAMLSAQVTTSGINGRITNDKGETLPGAT
                     IVAVHIPSNTMYGGISNDKGWYSIQGMRVGGPYKITVSFVGFASTEFENVQLSLGESS
                     NYNASLKESSSQLSGVVISAVVRKNTSGGAASNFKQTQIASTPTVDRNIYDIAKLSPL
                     VSTSKAGGISIAGSNNRYNSFQIDGMVSNDVFGLAGSGTNGGQTGANPISLDAIQEVQ
                     VLISPFDVRQGGFTGGGINAITKSGTNQFHGSAYGYFTNQDMYGKWSQVKEEEVKLAE
                     ESTKSYGFSVGGPIIKDKLFFFANTEYKFDTYPSDYYAGYKQDYISADEAQVITDRYS
                     DITGFSDSYGRRNLETKGLSILGRLDWNINKNNKFSLRYQYNDSYKDVYGASANTYYF
                     ANSGYRIIDKTNSLVAELNSHISNSLYNEARVGLTMVRDDREVAYQGPNITIENLGGV
                     LTDTAGWTQGPTMAYIGTEYSSGANTLNQDIYTIEDNLSWYKGNHTVTFGTHNEFYNM
                     YNLFIQASNGAYYYHSLNDFLNDNAYKFGYNYSDYDLTGSYKWAATVKAGQLGFYAQD
                     KWSISNNLDLTYGLRLDIPTFFNKPSTNAEFNESNYATAYDVKTGDVPATTVMASPRL
                     GFRWYANDSHKTLVRGGTGLFTGRVPFVWLSNNWSNTGIEMKGTTITSNVPSFATFGN
                     DPVAAMNSASGSASKPTINTVSKDFKYPQVFRSSLALEQVLPGDVKVTLEGLYSKTLN
                     NVWWENLALTDTLGKKVYAVSADVENSAATYWSRNTGSYYAIINLKNTNKGYTYALSA
                     KLEKTFDYGLDLMASYTYGHAFSVNDGTSSVAYSNWKYNYAINPNNADELSYSLFDVP
                     NRILAAVTYTSPKYLNKRLNTVVSLVYNGSNGQRYCLTMNESKDFNGDGQYGNSLLYI
                     PTKDELAKMTFTSEEDRAEYETWIEGDKYAKDNRGQFAERYSNSAPWENHFDLHLAED
                     FYYMSGKDGKVSLTVDILNIGNLLNKEWGANYSSYYNVQILKVTSLTTDENGNKIPTY
                     SYQGSEVTKSDFYSRWHAQVGLRVTF"
     gene            35810..36580
                     /locus_tag="PKI35_01260"
     CDS             35810..36580
                     /locus_tag="PKI35_01260"
                     /EC_number="1.1.1.320"
                     /inference="COORDINATES: protein motif:HMM:NF005381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-benzoin forming benzil reductase"
                     /protein_id="HNX43033.1"
                     /translation="MKYFIITGTSRGIGEAIARKLLMTGNSLICTSRSLNEDLTETAS
                     NMSIPLYYHPCDLSRTSSAMEFIKTAFRIIDLKNAESIALINNAGMLAPIGPAGNLKP
                     LDIERHYKTNILAPVNLTNEFIKRTQTLTLQKVILNISSGASGHPYHGWSVYCSSKAA
                     LEMFTRTVALEQETEKHPVIVFALKPGIVDTSMQSLIRRSDPELFRDRDKFVKLHDNG
                     LLVKPETVADAIVSAMLSGNIPQGGILTLDDLIRISSL"
     gene            36621..37190
                     /locus_tag="PKI35_01265"
     CDS             36621..37190
                     /locus_tag="PKI35_01265"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNX43034.1"
                     /translation="MLQALNQFDTSLLLYLNSLHSPFFDHVMWLASDKFIWAPLYIWF
                     LWLLYREYPKRFWLILASVALMILAGDQLSRLFKDMVGRYRPTHEPAIQQLVHTVNGY
                     TGGAYSFFSGHATNSFAVALFMIMMLPEKRRYMLPLTLAYAVLVSYSRIYLGVHYPID
                     ILTGAVAGSFIGWGASKAYRYTDLRLSRK"
     gene            complement(37211..37678)
                     /locus_tag="PKI35_01270"
     CDS             complement(37211..37678)
                     /locus_tag="PKI35_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNX43035.1"
                     /translation="MRAKFITLQREIDEIIEKCQVCYVGMADENGMPYVLPFNFGYKD
                     GVIYLHSSRKGMKIDILKKNPGVCVVFSADHQLHHQSEQVACSYSMHYRSVQAFGDVE
                     FVEDDNQKVEILNIIMSNYTDREFRYNPPSLREVCTYKIKVKRFSGKVFGYVR"
     gene            complement(37724..38074)
                     /locus_tag="PKI35_01275"
     CDS             complement(37724..38074)
                     /locus_tag="PKI35_01275"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNX43036.1"
                     /translation="MKFILRILVSSLAVFFAAYLLKGVHLNGFPTAVLLAIVLGLLNA
                     FLKPLLVLMTIPFTVFSFGLFLLVINAFIILIADKLLAGFAVDGFMTALIFSIIVTLT
                     TWILETLAGEKKES"
     gene            complement(38085..41012)
                     /locus_tag="PKI35_01280"
     CDS             complement(38085..41012)
                     /locus_tag="PKI35_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43037.1"
                     /translation="MKVFVCPKKKPCFKWFLIIQVLISCQVANAQYYNLGQDPGSIQW
                     RQIRLKNFRLVYPGEFESKAQRMANILECTFPLDTFSLTRPPSSIPVILHTSTVEPNA
                     FTVWAPKRIEMYMCPSQQTYAQDWMEQLAIHEYRHAIQVSMLDQGFTRILSKITGEQA
                     LAAVLGLFIPTWFMEGDAVSVETAISHSGRGRVPSFEQAIRAQVMQKGIYTYDKAVFG
                     SYKDYVPNQYELGYQIVANTRRLYGARSWEMVLSNVARKPYTIVPFDRSLKSVTGMGK
                     KALYGQTLTYLDSLWRIQQSKTVLSERSPLPVKTAKTYSLYKYPQYVNDSVFIAERTT
                     LDDISRFVLIDRAGKEKIIVTPGFFSADYFTVNSGFGTGYSLNKPGSFTADNISLVGG
                     VLAWTEKSADPRWQQRNYSVIKVFDFKSGKTRQLTDRSRLFAPSISPDGKSMAAVRVT
                     NHNICSVVILDMLTGDIMKVLCSSPDEFYMTPSWSPDGHMLVFTVLNNSGKSIRMLNV
                     NTVTSRTIVKPGFTDIANPMIAGGYILYNGSYSGIENIYAVDTASGNRFRVTSAEFGA
                     CNASFNPHLNRIVYSEYSAGGYHPVESAFDPAGWKPLEEVQDNSVSLYKWLLPQESGV
                     VDSSSIGNRVFDSEPYKKACHLFRFHSWAPAFIDFNSSYLNSGVSVASQNELSTATTL
                     AGYDWDIAEGVGRYRLNFEYAGWYPVLDVSVTEGKRAAYEESDEGKSHRYTWRETSLN
                     GGVKIPLCFIHGKYYSGLQPSVRTTWTNITHNTSADSGRISGTINTIDYRIYAYHYIK
                     QASRDIYPRWGQAIDLNLRHSPFGANELGNITSAETFLFFPGILKHHGLRVYAGMQYR
                     QEGDYSYSNLVNSPRGYNALKNNRMYSAGLTYKFPFLYPDYSAGDVAYFKRVKGALFY
                     DLTYAGDAGRFDRYESTGAELTTDVYLFRFLFPFDLGTRIGYLPQKSEWFLDFLISVN
                     FVL"
     gene            complement(41129..41503)
                     /locus_tag="PKI35_01285"
     CDS             complement(41129..41503)
                     /locus_tag="PKI35_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX43038.1"
                     /translation="MKRLRILLAEDDEMIEKITSYYLKKCGHDVDVARNGSEALRKFS
                     NEHYDLILMDIQMPEMDGLQAVKEIRRMEQDNQQKEHTTIIAITTNANKAECIEAGVD
                     GYARKPFSLEEFNTLFYQHNLV"
     gene            complement(41553..43280)
                     /locus_tag="PKI35_01290"
     CDS             complement(41553..43280)
                     /locus_tag="PKI35_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43039.1"
                     /translation="MKYRLLTWTLLVFLLCSGLTGFSRAGLKICSAGNHLSWDDPAAW
                     RIVQNGTTGAVTPQGDDTIMVRHEILMYNNFELNTGKLIIEKEGSLLAPGCTVNISSD
                     AAGECSGRLEVLNLTVKGSASFLLMPEGKVTVNGLLHVDAGALFTLQDSKNKQGSLIT
                     YGPVEGNVSAGYSVPANENLLVSSPVSGAESGVFLNMYLRNYNEQKSGWGEYIVPTTV
                     PLNPMQGYEVLSTYEDYRNFTGVPLSGEQKIAITSDGDGWNLVGNPYPSSIDWSLLGN
                     GYDHGDFPPGMSTIYYWDPSGSGNFSVFCAGEEPICINNGSPVVHPMQGFFVKAGVSG
                     EISTTNKMRIHAEDGDFVAPLPLSALKFKIEGNNFSDEAVVRFNPDASGDFDIAYDAY
                     ELQGFNTAPGIYTTLADGSKLAINTLGSVSSGSIIPIDVIIPYAGISVIKVQGASAFE
                     FRYPVYLEDESTGIYTDLRADSVYSFESENGDPVRHFKLHFALPGGIQENETNTISVT
                     AGGSAIDIKDAEGRSGIIEVFTVDGKRIFRSAVTSLAAVKIPVSPGRVYITRIETGEK
                     VETCKLYCY"
     gene            complement(43653..44396)
                     /locus_tag="PKI35_01295"
     CDS             complement(43653..44396)
                     /locus_tag="PKI35_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HNX43040.1"
                     /translation="MNANSTDSPDSFTLISNSILYEDNHLLIVNKMPSQIVQGDKTGD
                     TPLSETLKGYIKEKYNKPGEVFLGVVHRLDRPVSGAVIFARTSKALTRLNLMLKNHEI
                     HKTYWAVTGNKPPAGEGLLIHHLVRNEAQNKSYVVAGPRKGSQVAELHYRLIASSERY
                     FLLEIELHTGRHHQIRAQLSAIGCPIKGDLKYGSPRSNPDGSIHLHSRKIDFIHPVSK
                     LNISVTAPPHPEKVWNIMVNAYNSGLPKQ"
     gene            complement(44377..45192)
                     /gene="panB"
                     /locus_tag="PKI35_01300"
     CDS             complement(44377..45192)
                     /gene="panB"
                     /locus_tag="PKI35_01300"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNX43041.1"
                     /translation="MSKFSSVSKVTTHVLQEMKLKGEKIAMLTAYDYSMARLLDRAGI
                     DVILVGDSASNVMAGHTSTLPITLDQMIYHACSVIRGVSRAIVVVDMPFGTYQGNSRE
                     ALQSAIRIMKEAGADAVKMEGGVEIIESVERILSAGIPVMGHLGLTPQSIHKFGTYVV
                     RATEEPEARKLVDDAKKLEEAGCFSVVLEKIPAKLGAEVASKARIPIIGIGAGPDVDG
                     QVLVLHDMLGITTEFSPRFLRRYSNLSEEIINSVKSYISDVKSLDFPNEREQY"
     gene            45397..46425
                     /locus_tag="PKI35_01305"
     CDS             45397..46425
                     /locus_tag="PKI35_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43042.1"
                     /translation="MRLSAMKRIPFLILLLPFVFSLASCETDFDITAGYKDITVVYGI
                     LSQNDSIHYLRINKAFLGSGNALEYSHIPDSSSYGGNIEVTLEEVTGDGTGNILTFDT
                     ITLAIKDSGAFYYPDHLYYIAKGTLNPTATYNLKIVNKKNGKVVSSSTKLVQDFNISN
                     PNYWLKTLNFKKANTTTQKFEWESAENGRLYQPVLYYFYKETSSPGDTIVRKVEWKFP
                     AKRSSSLDGGKAMSVEYLNADFYARCTAQIPYADAAAEEAVIARVADHLELIFTVVGD
                     DFNTYIEVNEPTTGVLMEKPEYSNITNGIGLFSSRYQKKSDYMLGKDTQLDLYNNTNL
                     KFVRPVGD"
     gene            46880..48793
                     /gene="dnaK"
                     /locus_tag="PKI35_01310"
     CDS             46880..48793
                     /gene="dnaK"
                     /locus_tag="PKI35_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNX43043.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNEPVVIPNSEGRRTTPSIVAFTE
                     NGERKVGDPAKRQAITNPHKTVFSIKRFMGETFDRVTKEVQRVPYTVVRGDNNTARVK
                     IDDRMYSPQEISAIVLQKMKKTAEDYLGQTITEAVITVPAYFSDSQRQATKEAGEIAG
                     LTVKRIINEPTAAALAYGLDKKNKDLKVAVFDLGGGTFDISILELGEGVFEVKSTNGD
                     THLGGDDFDHRIIDWLADEFKKDEGIDLHKDAMAMQRLKEAAEKAKIELSSSTQTEIN
                     LPYIMPVDGIPKHLVRTLTRAKFEQLIDDLVRSTIEPCRKALSDSGYTASQIDEVILV
                     GGSTRVPVIQKTVEDFFGKTPSKGVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTPLS
                     LGIETLGGVMTKLIESNTTIPTRKSEVFSTASDNQPSVEIHILQGERPMASGNKSIGR
                     FHLDGIPPAPRGIPQIEVTFDIDANGILHVTAKDKGTGKMQQIRIEASSGLSEAEIKR
                     MKEEADANAESDRKMKEEVDKINSADALIFQTEKQLKEFGDKLPADKKAPIESALAEL
                     KEAHKNHNIAAIDDAMNRLNTAWQAASEELYKASQGAPQDNPGAGQQQQQQQGGGSQP
                     DQEVTDVDFEEVK"
     gene            48987..51944
                     /locus_tag="PKI35_01315"
     CDS             48987..51944
                     /locus_tag="PKI35_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNX43044.1"
                     /translation="MRKFTLFAVTVTVFLFFGFLKVGNSQVVISQMYGGGGNSGSTYT
                     NDFIEIFNRGTSNVNLAGWSVQYASATGSSWQVTTLTAVSLAPGQYYLIQEAAGSGGS
                     VPLPTPDVIGTIPMSGTNCKVALCNSSLVLTGACPTSGQIMDLVGTGTANCFEGSAAS
                     VAMSNTTSVIRASDGCQDSNDNSLDFSSSAPTPRNTLSPTHTCSTTAAALPTLDPGTG
                     YYYSTQNVTITCATVGASIYYTIDGTDPNNTGNGTLYTSPVAISSTTTLKARAYATGY
                     DPSSIASATYTFPTVVNTIAALRAGTPGEIYVYTGQGVLTFQQSYRHQKFIQDATAAI
                     LIDDNAGKITTTYAPGDAITNITGTVTEFNGMMEFTPEADPGAPASTGNTITPEEVSL
                     GDLLGNWENYESELIKVPDVTFTSPSGNFANGTVYPVTDINGTSGNFRTTFYDVDYIG
                     TPVPSAREDLVIIPNSRSDGDYITSRSLSDFTYNSSDNIVINEIMYNPPDAGNDTIEF
                     IELYNKGVTDVNLNGWYFSQGIDYVFPDTTISAGDYFVIARNALSFDYTFGFTPAQWT
                     SGILNNFGSEIEIKDAIGNVKDYVHYLPDLPWDTLANGHGPSLEICDPNADNSLPESW
                     SASRVFAAVNTAGDSIFATPNARCGSGANLVITEIMYNPPESGTDTLEFIEIYNNGNS
                     VNLEGFTFTEGVTLTFPSVDLPSGNYLVVAANAAAIQNTFGVSAIQWTSGALSNSGEA
                     ITLQDPYGYIIDQVTYDDQLPWDSIADGYGPSLTLCDPNSNNALAENWKASVELAAVN
                     AAGDSVYATPGSGCVNPPAFANFTGYPTTLLEGEYVQFTDLSANNPISWEWSFPGGTP
                     ESSTEQNPNIRYNTFGVYPVTLTATNVYGSGTLTKTDYIHVDIDGIARIPSDAVRIYP
                     NPAHEKVYVTNPTDKSINISVLSLLGSEVVSIKSAAEIIPVDMSRFPDGIYLVRLTTN
                     DGKTITTQKLILK"
     gene            51962..53650
                     /locus_tag="PKI35_01320"
     CDS             51962..53650
                     /locus_tag="PKI35_01320"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HNX43045.1"
                     /translation="MRKKLLALAAAFLAAAGLSAQNAWINEIHYDNTGTDADEFIEVV
                     IENPGSYTLSDFAVDLYNGNDGKTYSQKLLSEYTVGATSGSFTFYYLNYTAAGLSIQN
                     GAPDGLCLSYQGTVIPAQFLSYEGTLTAVDGPASGMTSVDIGVSEVNSPVGQSLQLSG
                     TGSQYAAFVWQPPATATMGQVNNGQVLGTYTPDPEPSAYPASFTATGQGLSALLTWVD
                     ATGTQLPAGYLIKASTSSAITPPVDGTPEADDEDLSDGKGAWNVTFGAQSHTWNGLAA
                     STLYYFRIFPYTNYGQYIDYKTDGTAPAAEATTQAIVKFYNFDDGLEPWTQYSVMGDQ
                     IWVYDSIHGIDGSGCAKMSGYSSGNFENEDWLISPAIDLTGTHEAMFRFFSGENYGDG
                     SNPMVILLSTDYTSGNPATSGTWIDVSQGITLSPGSWTWTPSEYIDLSSYDQPNVRIA
                     FKYTSTTNDAPTWEVDDVLITASSGVGMEETPKDVAFEAYPNPCTEWFFLNVAEAGDY
                     SFSLINSSGKTVINRQVQDGATRVDVKSLSPGLYILSVYNHQTGTFSTSRVVVR"
     gene            53762..55402
                     /locus_tag="PKI35_01325"
     CDS             53762..55402
                     /locus_tag="PKI35_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43046.1"
                     /translation="MLRNCLNILLLILIPVMVLPQTAQPVRFEAPTRSGTDPFNHVAC
                     GDQGVCLFYPTIEESGKDSVSWSFYLLDRNFQKIWQQRIPLREDVSFLKSIYRNNCLY
                     LLFHDTKRNDKGNISVFFFIPSKQILTEHHSDIPYKADIVDFEVLNDLIFIGYNEKKS
                     KPGLIAFSLIDGQKRNFEISAEDDALLLDVAVDSIYKGVYATYKVQKTATRNNLYINK
                     YDLSSALRSTTTFEGEVEKRIINSAEYIPLSDTQGLVAGCYGYSNRGRRTYDYYNDYY
                     NYYYYNSLYNRPYTYDANKDNTPVSDGYFSSLVGGSSPPALKYYNFVDFNNALKITSD
                     MDILRLRRRNEKKEKTGLTGEDNTVDDDNNSLNYRILMHSIIASDGKFLLLGETYYPE
                     YHTMTQMVYDYYGRAFPSTYSVFDGFRYCNAFLAAFDSSGVKVWDNGMEMRDILTTYL
                     NHKMNYIFDGDEMTLFYNANGKLAVKTIKGPETVEHTEYVTIAPKRQTDEVTSEYLGT
                     MEHWYGKYFLASGYETIRNNFLTENKRNVFYLNKMAFE"
     gene            complement(55617..57137)
                     /locus_tag="PKI35_01330"
     CDS             complement(55617..57137)
                     /locus_tag="PKI35_01330"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNX43047.1"
                     /translation="MKKSPAVGCILAGFIFLSVISLKPSDAFAQKERTQSEVFSGYGY
                     FGFSIGASEYLGDLNKDDYFNSKLGWGAGLLGGYQISPVFGLRGQFVAGNLKSERTDI
                     HFRKLNSQFLDLSLNLTLNINELFGRYNSDRLFNFYLFGGPGISSYHSLVTDLDDVKL
                     YETDGRTNEYIMPYGFGMNVKLSSRVDLNLEYGDHITFSDDKLDFLASTDRRDHYTYI
                     SAGLTLRLYQKDSDDDGIIDRKDLCPDAPGLLVFQGCPDKDGDSIIDENDACPDIAGL
                     VEFKGCPDTDNDGIPDKDDICPTEAGLKALNGCPDKDGDGIADKDDRCPDVPGKNDFE
                     GCPDRDGDGIPDIDDSCPDMPGTLVYSGCPDTDGDGIPDNKDQCPRLAGVAENEGCPK
                     AKIGLPNVVYFDTDKHVVIDKYKAALDELAGFLTANPGIVINVAGHTDSRESEGYNMA
                     LSEKRAGYVVTYLIKIGIPKSRVEKSFFGETKPAASNETPEGMALNRRVEISEIKH"
     gene            complement(57354..57896)
                     /locus_tag="PKI35_01335"
     CDS             complement(57354..57896)
                     /locus_tag="PKI35_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNX43048.1"
                     /translation="MRKLQMNELNRVSVDKFHELEKMPVVIVLDNIRSQHNIGSVFRT
                     ADAFRVEKIVLCGISATPPNREIQKTALGATESVSWSYSADVTETLSGLKSEGYKIIA
                     VEQAEGSISLAEFISPAGIKLALVFGNEVNGVSNEAMAQADQCIEIPQFGTKHSFNIS
                     VTAGIVLWEMFNKLLPQMNF"
     gene            58122..60725
                     /gene="mutS"
                     /locus_tag="PKI35_01340"
     CDS             58122..60725
                     /gene="mutS"
                     /locus_tag="PKI35_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNX43049.1"
                     /translation="MTPLMKQHHAIKAKYPDALLLFRVGDFYETFGSDAVKASEILGI
                     VLTKRANGAAAFVELAGFPHHALDTYLPKLVRAGYRVAICDQLEDPKTAKTLVKRGVT
                     ELVTPGVSYNDKILENKENNFLACIHLLPASSGVSFLDISTGEFLLAQGSNDYIDKLF
                     QTFRPSEVIVQKNKRQQFTEVFGNRFYLSYFEDWVFTSDFASQLLTDHFGTTSLKGFG
                     VSELTNGIIAAGAVLHYLSETQHDRVRHISKISRIEEDHYVWLDKFTIRNLELLNTAD
                     ENATSLLSVIDRTISPMGSRLMKRWILLPLKDKQPVIERHDMVECFIRNESFAQKIEH
                     ELKVIGDLERLISKVAVGRVSPREVLQIRRALQAIIPVKEACAASDNAALRHLADQLN
                     PCELISEKIGREIHPDAPVSVVKGMVIASGVSEELDELRNIQYSGKDYLVQIQQRESE
                     RTGISSLKVGFNNVFGYYLEVTNSHKDKVPAEWTRKQTLVNAERYITEELKQYEQKIL
                     SAGERILELETQLFNELVLSLSGYVEPVQVNAQLIAQLDCLLSFARIAHEYRYVKPEV
                     NDGPAIDIKQGRHPVIERLMPAGEEYVANDVLLNNDQQQIIILTGPNMSGKSALLRQT
                     ALIVLMAQIGSFVPAKAANIGIVDKIFTRVGASDNLSSGESTFMVEMNETASILNNIS
                     DRCLILLDEIGRGTSTYDGISIAWAMTEYLHEHPLFRAKVLFATHYHELNEMADTFHR
                     IKNYHIAVKEVGNKVIFLRKLTAGGSEHSFGIHVARMAGMPGGVLRRAEELLPLLEKK
                     HSNEQLAGNAALKKASKAGNDPYQLSLIQLNDPLLEQIRDDIINLDINTLTPVEALMK
                     LNQIKKMLEKY"
     gene            60781..60853
                     /locus_tag="PKI35_01345"
     tRNA            60781..60853
                     /locus_tag="PKI35_01345"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60814..60816,aa:Gly,seq:gcc)
BASE COUNT        16638 a        15209 c        13643 g        15332 t
ORIGIN      
        1 gcataatttc taaaaaaatc aacatctgaa tgcgataatt ttcctaattc gcaggcactc
       61 tgaatgcagg cattaattcc tcattttgta gcaattgcat agatatcgaa tgccgcatcg
      121 ttgtattcct tcaggcggaa atcatccacc gttacgccgc ttacaatgcc gttattgctt
      181 ttcagcagtt tacggcggtt catccggttc ataatatcgt aaggaatctt aagcagccaa
      241 cggggcatcc ggtattgaag attcagcacg tcgaaccgca tgattttccg cactgattgt
      301 ttattgcggt cataatacgc catcacctta tcatcgccaa atacgccttt gagcgtcacc
      361 tcctgaaatt tccccgacag cagggccgac aactgctttc cggtatattc acgcacatgc
      421 cagggattgc gggtaagcga catgagcctg ttgggcgtag aaacaaccag tttcccgccc
      481 tttttcagaa cgcggtgtat ttcgctcaca aattccttat catttttaat atgctctatt
      541 acctggaatg tgatggcaac atcgaatgaa tcatcaggga tgccggccag cgaaggaaga
      601 agcatctgcc ggaattcaat atttccggaa gcttccagca ccggttcaaa cttgtcaatc
      661 gccaggtacc tatccgctga tggcgccagc aggcggatgc cataaccttc gccgctgccg
      721 agctcaataa cattgccttt gatcagcgcg cgcgaggttt cataagcaaa aaggcaccgg
      781 ttatagatga cctgttcgga aggatcgagg acagaactac gttcagcggt ttgcatagca
      841 aagaattatt tttgttgaat cgagagcaaa agtataaagt aaaaagaaaa tagaaaacct
      901 gccgctttta ccttttctgc ataagcatgc taagccggtt atcaaacatt ccgtaaatca
      961 atgaataaca tccggttaat gaaagcaact tcacaaaacc ggcaaaaagt ctttatttca
     1021 tattcccgga aaattatcat gcgtgatttt tttcgcaatt ttttttcgat ttctttctaa
     1081 taaatttaac tatgtgagtg aatataaata attattttcc taagtttacc tgcccttatc
     1141 aaaaacaccg gcaattcagc aaaacacaat taacctattt tttttacaaa tcgctttaaa
     1201 tcaatactat gaatcctcaa ttactatttc gtaatactga tacaggatca aaagcgaatt
     1261 tctcgaagaa gaattcctta aaagttttga ttcttgaaga attctaccgg aatgggcgaa
     1321 agtctattcc cgagctgagt aaaagtatcc agatgagcac cccgacgatc acccgggcca
     1381 ttgacgaatt aatggctgaa gatcttattc ttgaagaagg catcgggtct tcgagcggcg
     1441 gacgcaggcc taatctttac gggatcaacc cgacctcacg ctatgtaatg ggcatcgacg
     1501 taaaccgttt ttatgtcaga atcgggcttt ttgatttcaa caaccaaccc gctgccgaaa
     1561 tcaaagtatt taacgagggc ctggagacgc aggagaacat ttcggccttt ctgcgaagat
     1621 cggttgaaga atatatccgt gaatcaggca tcgatgagga caaactgatc ggtatcggca
     1681 ttgcgatgcc cggtcttatt gatatccata ccggtattac ctatagttat ctccacgatg
     1741 aaaagccggc caccatcatg tttgaaaacc tgtttgacca ccccgttttc gttgaacacg
     1801 acaccagggc aatggcgctg ggtgagcagg cattcgggct cgccaaaggc aaaagcaatg
     1861 tgctgtgcct gaatatcggg tccgggatcg gcctcggcat gatcctgaac ggcaagcttt
     1921 atctcggcca ttccggattc tcgggcgaac tggggcatat acaggttgat gccgaaggcg
     1981 acctttgcta ttgcggaaaa atcggatgcc tcgaaacggt agcttccgga accacaatga
     2041 ttaaaaacgc caggaaaaaa cttgaatcag gagccacctc cctgctcagg acactggttg
     2101 atgaaaagct ggaaaaaata aacagcgagc tgatactgga agccgctcag cgaggcgacc
     2161 agtttgccat cggactgctt tcgaacatcg gcgaatatct cggcaaaggg attgccgtgc
     2221 tcatccatat cttcaatccc gagctcatta tcatcggagg cgaactcacc aaggccgaca
     2281 actacctcat tgatcccatc cagcagaacc tgaataaata caccatctcg aagatacgcc
     2341 gggatgccca gataacaact tccagccttg gcaacaatgc agggctgatg ggaacagttg
     2401 cgctcgtgat gaacaaagcc ttcagcaggg aataatgacc gtccgtgcct tttcgtaact
     2461 tcatttgccg gctcatcgaa aggcctgtat tttttattaa atttaaattc aattttaatc
     2521 gttttcaaca tgaaaaacag gcgctgcatt ttcgtaatgc tgttgatatg ctttgcaaat
     2581 ctgtcgtttg cccagcaaaa agctgttaac gactgggaaa atccggggct cacatcggtc
     2641 aacaccgaga agccgcatgt caccatgatt ccgtttgcaa acgaaagcca ggcgctgaaa
     2701 aacaaccggg cagaatctcc tttttataaa tccctgaatg gtaaatggaa attcaacttt
     2761 gccgggaatc cggaacttgc tcctgcagat ttctggaaaa acggctataa ctgctccgga
     2821 tggaacgata ttgaggtgcc ttcgacctgg gaagtacagg gatacggcta tcccatttat
     2881 gtgaatatcc gctatgaatt ctttacggat aacccgaatc cgccaatggt tcctcacgat
     2941 tacaatccga ccgggtccta tatcactacc ttcacccttc ccgaaaactg gcagagcaag
     3001 caggtgttcc tccatttcgg cgctgtaaaa tcgttcttct acctttggat taacgggcaa
     3061 tatgttggcc tgagcaagga ttcaaaaacg cccgccgagt tcaacatcac aaattacctt
     3121 aaagacgggg agaataaaat cgcgatgcag gtaatgcgct ggagcgacgg ctcctacctt
     3181 gaatgccagg atatgtggcg catgagcggg atcaaccgcg acgtgtattt attcgccagg
     3241 cctgatgccg gcatccgcga tttcttcgca gccggcagcc tgactaataa ttacgaagac
     3301 ggactgctcc gacttactgt ctattttgac ctgaagcgca acgccggcat ttcggaccgt
     3361 aaacttaaag tcagcctgtt cgataaaacc acatcgtcgc ctgtattcag cgatttacgg
     3421 gaattatcgg aaatcggcgg aaaaaccgac agcctttatc tcgaaaagat tatcagccag
     3481 gcaaagcaat ggagcgccga atatccgaac ctttacacgt tggttctatc gttgattgac
     3541 aatgacggca acacccttga atcggtttca aaccggatcg gattcaggac ttcagaaata
     3601 aaagacggct tcctactggt caacggacaa aaaatcagga taaaaggagt gaaccgccac
     3661 gagcatgatc ccctgaaagg ccatgttatt tcacatgagc tgatgctgaa ggatgttcag
     3721 ctcatgaagc agaacaatat caacgccgtc cgcacctgtc attaccccga cgacccatac
     3781 tggtatgaac tctgtgacga gtatggcctc tatgtcatcg acgaggccaa tatcgaatcg
     3841 cacggcatgg gatacaacct caaccgcacc ctgggcaacg accgccgttt cctggcggcc
     3901 cacctcgacc gcacacgccg catgatcgaa cgcgacaaga accatccttc cgttatcatc
     3961 tggtcgttgg gtaatgaagc cggcaacggt tacaacttct acaatacgta tctgttggcc
     4021 aaaagccttg acaaatcgcg tcccgtgcaa tacgaaagag ccggtctgga atggaacacg
     4081 gatatttact gcccgatgta cgaaagtgtg gaaggtatac tgaattacgc ctccaaacat
     4141 caggatcgcc ctctgatcca atgcgaatat gcccatgcca tgggtaacag cagcggcaac
     4201 atggcggatt attgggatgc catcgacagc catgaccagc ttcagggtgg cttgatctgg
     4261 gactgggtag accagggatt gctgaagttc gattcgatcg gaaaaaagtt ttgggcatac
     4321 ggaggcgatt tcggcccgaa ggatgttccc agcgatggca atttctgttg caacggcctt
     4381 gtttttcccg acaggacccc tcaccccgcc ctcaccgaaa tcaagaaggt atatcagtac
     4441 gttgggttcc ggctggcgaa cgaaggtgtt ccgtcgatag aaatcagcaa taaatatgct
     4501 ttttacgacc tggcaaatac ccgccttgtc gccgaagtca tggttgacgg cgtttcgaag
     4561 gaaaaaatcg agataccggc tccgatcctt ctccccggcg aatcgaccgt gttgcccgtg
     4621 cctcttgcca cgttgttccc gaaaaattca gaagtattcc tcaatgtatt cctgaagact
     4681 gatgacgaca ggcctttgat gggcaagggc tttgttcttg catcggaaca gttgccgttg
     4741 ccggcatcgt cactcaccaa gccggctgct tcgcccgtta tcataaaaac atcctcgctc
     4801 ccacccctca aaacgaccga aacaaatgaa gaaattactg taaacggaag aaatttttct
     4861 ctgtccatca gcaagacttc aggcgccatc gctaaatttc tttacaaagg gcaggaaatc
     4921 attgtcaacg gcccggtacc caactttcgc agggctccga ccgataacga cgccggcaac
     4981 cgaatgttca ggacatgcga agcctggtat accgacagtg ggaagagaaa cgcaggttcg
     5041 gtaacccttg ataattcaaa tgcgcatttc ccggttatca ctgttgttta cgatctgcct
     5101 gccaccggca gccaggttat tgtcaaatat tcagttacag gcaatgctga cgtatttgtt
     5161 gattacagcc tcaaaggcgt agaccccaag ctccccgtta tcccccgggt tggattaaac
     5221 atgcagttga acggagcgtt caggaatgtg gaatggtacg gacgcggatg gggtgaaaac
     5281 taccaggacc gcaagtcagg atcattcatc ggaatttaca aagggactgt tgatgaattg
     5341 tatgtcccgt acgtccggcc gcaggaaaac ggctaccgca ccgatgtccg gtggctttct
     5401 gtaaacaatg gaaccggatg cggtgttcta atccaaggac agccgctggt atgtttcagc
     5461 gccctgccct atacctatga cgatatgaaa ggattcagcc atggcggaaa gcacatcgat
     5521 cagttgcctg acaatccttt tgtagatgta aacattgact tgctgcaggc cggcgtaggc
     5581 ggcgatgata gctggggagc atggccgatg gaaaaatacc ttcctgaagc aaaagattac
     5641 cactattcat tcaggatgag gccttattta caaaaagaaa acactcctgt taaactctgc
     5701 aggcaacaaa ttagttttta atcaattaaa cactgattgt tatgggactg tcgtataatg
     5761 ttcttcatcg tcaaccgata gaaaaagaaa ttgaaacaga tgcctttgtc acccgtttcg
     5821 aaaaaatgcc gttacggatt ctcgggtctt cttccgaagg ttcgttgatc gttgcccgcc
     5881 gtatcgccga cctgatcaaa gaacgaagca agacaggcag aaacgcggta ttggggttag
     5941 ccacaggttc tactcccctc aacatttacc gagagcttgt acgcatgcac cgggatgaag
     6001 gtcttagttt cagcaatgta accaccttca accttgacga atactatccg atactgccca
     6061 ctcatataga gagttataat catttcatgc atgaacacct gttcaggcat atcgatataa
     6121 agccggagaa tatcaatatt ccccggggcg atatttcccg tgaacaggta gccggtttct
     6181 gcagggagta cgaacaaaag attgccgggg cggggggcat tgatatccag atattaggaa
     6241 ttggacgtac cggccatatc ggttttaatg aaccggggtc ctcctttacc tcaaaaacga
     6301 ggttggtgac gcttgaccgg ctgacccgtg tggacgcggc ggatgctttt aatggtgaag
     6361 aaaatgttcc ccattttgcc atcaccatgg gcatcggcac tatcatccag gcccgtgaaa
     6421 tctacctgct ggcctgggga gaaggcaagg cggaaatcat tcagaaagcc gtggaagggc
     6481 ccgttaccga cctcgtgcca tcgagtttcc ttcagaatca cgccaacacg actatcatcc
     6541 tcgacatggg agccgccgcc gagctgacgc ggttcaaaac gccctggctc gtaggcagtt
     6601 gcaaatggga tgaccggatg atacggcagg ctgtattatg gctttgtatg aaggctggaa
     6661 aacccatcct gaaacttacc gaacgggatt ataacgacaa cggcctcagt gagctgatta
     6721 ccgatcatgg cccgttcagc aagattaaca tcaacgtttt taacgacctg cagcatacca
     6781 ttaccggctg gcccggcggg aagcccggtg ttgacgactc tacccggccc gaacgcgccc
     6841 tgccctaccc aaaaaaggtg gtcatcttca gtccccatcc cgatgacgat gttatttcga
     6901 tgggcggcac atttgcccgg ttggttgagc aacatcacga tgtgcatatc ggatatcaga
     6961 cttcgggaag cattgccgta gccgatgatg acgccatccg ttacctcgat ttcgttcagg
     7021 actatgaaga tatctacaaa acggaatgcc atgaaggatc ccagaagtac cacgaaatcc
     7081 ttgaatttta ccgtcgccgc aaaaaggaca ctattgagcc aaaggaatta ctcgagctaa
     7141 aagctgccat ccgtcgtgga gaagccaggg cgtcgtgccg gtatatcggg ctgccggaag
     7201 agaatgtaca ttttctgaac ctgccttttt atgaaacggg aacggtaaag aaaaacccag
     7261 ccggcgaagc cgatgtgatt gccatcatgg aattcctgag agctatcaag cctcaccaga
     7321 tttacgccgc cggtgacctg agcgatcctc acggcaccca tcgtgtttgc atcgatatca
     7381 tccttgaagc gctcgaccgt atgaaggacg atccctggat agacgattgc cgggtgtggc
     7441 tttaccgggg agcctggcag gaatgggaac tcgacaaagt tgacatggcc gttcccctca
     7501 gccctgaaga ggtggagatc aagcgttatg ccattttcaa gcaccaaagc caaaaggacg
     7561 gggctttgtt catgggaagc gataaacgtg aattctggca gcgtgccgaa gaacgaaaca
     7621 agaatacagc aaagctttat gacaaactgg gcatggccga atatgaagcc attgaggttt
     7681 tcgtaaggta tgatgttaaa taacttctat taccctgttt ataacaataa ctaacacaac
     7741 ttaaaacact gcattatggc taaagacagc actctgccag tctcaggaaa aaccagttac
     7801 ataatcccaa tcactgttat cgggatactg tttttcatct tcggatttat cacctggctt
     7861 aatggaacgc tgattccatt cctgaagata tcctgcgaac tgaacaacgt ccaggcatac
     7921 ttcgtgaccc tggcattcta tatcgcatat tttgttatgg cgcctccgtc gactgtggtc
     7981 ctgcataaag tgggatttaa aaacggaatg gccatcggat tattcatcat tgccattggt
     8041 tcactggtat ttatcccggc agcgcttacc cgtactttcg ggctcttcct aaccgggttg
     8101 tttattcagg gaaccggcct tgccttattg cagacagcat ccaatcctta tgtgattgtc
     8161 ataggaccgc gcgaaagtgc tgccaaaagg gttgccatca tgggtatttg caacaaagta
     8221 gccggcgcct taagcccttt gattttagga gccgtgatcc tgaaccagaa taccacggat
     8281 gtggtagaac agctcaagac tataaccggg gctgaccgca ttccgatgct cgaccttctg
     8341 gctcataaag tgattttgcc ttatatcatc attgccattg cgctcgcctt gctttcgatc
     8401 gcccttaagt tcattcacct tccggatgta gacacagacc aggaggaaga tacagcatcc
     8461 tccaacggct ctcataaaac cgcatggtgg caatttcctc atctttggat cggatttgct
     8521 accctgttct tatatgtcgg ggctgaagtt attgccggtg acacgatcat tctatacgga
     8581 ctttcacagg gaatccctct ggatacagcc cgcacattta ctacctttac gctcttatct
     8641 atggtactcg ggtacatcat cgggatcatc actatcccga aattcatcag ccagagcaaa
     8701 gcccttgcca tttccgcagt actgggagtt ctttttgccc tggcagccat cttcacaccc
     8761 ggattgacca gtgttgtctt catcgcatta ctgggattag ccaatgccat tatgtggcct
     8821 gccatctttc cgctctctat cgccgacctg ggcaggttta ccaaagcagg atcagctttg
     8881 ctgattatgg gcattgccgg tggcgctctg atccctctcg gatacgggaa actggtggaa
     8941 atgatcggca accagcaagc ttactgggta cttctcccca tctacatctt catcttctat
     9001 ttcgccgtta aaggtcacaa aatcaggaaa taatctgctg ttaaaccatt actcatgaaa
     9061 cctaccttgc tgatacttgc tgccgggatg ggcagccgtt acggaagcct gaaacagata
     9121 gatcctgttg gcccatccgg cgaaaccatt atcgattatt ccgtattcga cgccttacgc
     9181 gccggctttg ggaaagtggt tttcattatc cggaaagaca tcgaagccga ttttcgggaa
     9241 gtctttattt ccaggctttc caaacatgct gatatcgaat atgtattcca ggaactggat
     9301 atgatcccgc ccggatattc cattccggcc gaacgcacaa agccctgggg tacaggtcat
     9361 gctatcctga tggccaggaa tgcaatccat gaacctttcg ccgccctgaa tgccgacgat
     9421 ttctatggag caggagcctt cgacgtgatg gcaaaatacc tgaattcact aacgcccgct
     9481 actcaaaccg actatgccat ggtcgggtat gatctcggga aaacaatgag caaaaacggg
     9541 gctgtatcgc gcggaatctg ccgcaaagat gacagcaact ggctcatcga ggtaacggaa
     9601 aggacaaaaa tcacctatac cggcaacgga atagccgatg ttcaaccgga aaaggaacct
     9661 cttctactgc atgaaaatga tgtggtttcg atgaatttct ggggattcac gccagaatat
     9721 ttcaaccaga caggaaggct ttttgaagaa ttccttcagc agaacatcga aaagcccgct
     9781 tctgaattct atattccgct cgccgtcgat acactgatca aacaaaaaca ggccaggata
     9841 aaggtgcttc aaagccatga ccaatggttt ggcgtgacct acaaagaaga caagccgctg
     9901 gttgttaata atataaagca gctcatacaa agcggttact atcccgggaa actctggaaa
     9961 tagaaaaaaa aatggaaaaa gatatcagga gcatcatttc gcatttcctg agcgccggat
    10021 gcttttcgga agcaaaaccc ttgcttacgg gacacatcaa cgactcctac ctgattttta
    10081 cggccaacaa ccgccagccc gtttatttcc ttcaatggat caataattac atcttcaagg
    10141 atattcccgg attgatgcag aatattctgg aggtgacgcg tcatctggaa gcgaaacaat
    10201 gccggggggg caatgccgaa tgtactgtcc ttaagcttat cccctgcgac accggcgatt
    10261 ttttttacca ggatgatgcc ggaaatttct ggaggcttta caattttatc cccggcaccc
    10321 atgcttacga tgttgtcgac tctcctgaaa tcgcttatga aggaggcaaa gcattcggaa
    10381 aattttcatc cgacctcgcc gacctgcccg ccagccgttt gaaagatacg attcccgatt
    10441 ttcataacat ggaaacccgc cttgccaaat tttaccagtc ggtacgcgac gacaaggcag
    10501 gccgcaggaa gggtgtaagg cctgaaatag cttttgttga ggaacgcgcc gctgtcatgc
    10561 tttgcatccc cgaaatgatc cgcaatggaa aaatccctca aaggataacg cataacgata
    10621 ccaagttcaa taacatcctg ttcaacgaga agaatcatgc cgtctgcatc gtcgatctcg
    10681 acaccgtaat gccgggaagt attctctttg atttcgggga tgccatcagg acaggggcca
    10741 actgtgccga tgaggatgaa aaagacttgt ccaaagtaga tctcaacctt ccggtttacg
    10801 aagcctacac aaagggcttt atcgaaagca cccatacatc gcttacccgg gaagaaattg
    10861 ggcatctgtc gtttgccgcc cgtttcatga cctttatcat cgggctccgg ttccttaccg
    10921 attacctgga cggcgatccg tacttccgca cacggtacga agaccacaac ctggtgcgtg
    10981 cccgtgttca gttcaggctg gttgagaaaa tggaagagaa tgccatccgc atggatgaaa
    11041 ttgttcaaaa agccatcagc gaacagcaga aaaacagagt ttcactacca aaataagtaa
    11101 acgtatgaaa tttaaaaagc tttgcctggc aatagtaatt ctgcttgcag gattcaccct
    11161 tacggccgga aacgattttt cggtcgtacc aaaacccatg aaaacagaag ccggaaaggg
    11221 taaatttacc atcaagccat cgactgtcat actcactgat gccggaaaca aagagcaacg
    11281 tctgctctcg aaaatgctgg tacagaaaat cggcgatgct ggaggccccc tgctccgtat
    11341 cgaagacctt cccgccggca ggattcccaa gaatgctgtc gtcctgactt ttcaaggggc
    11401 tggcaatgat attcctgatg aaggatatac tctttccgtt acaaaaaaga atattttgat
    11461 ccgcgccaga aaagggcagg gattattcta tgggattcaa acgctctttc agttgcttcc
    11521 tccttccatc gaaagccatt cggacggcag gaatgctgtt tttgcagtta aaagcgttga
    11581 aatcaccgac atgccgcgct acccctaccg cggcatgcat ctcgatgttg gcaggcattt
    11641 tttcccggtg gaaaccatca agacctacct cgacatcatg gccatgtaca agatgaacac
    11701 ttttcactgg cacctcaccg atgaccaggg gtggcgcatc gaaattaaaa agtatcccga
    11761 gctcactaaa atcggggcca tcaggaaagg aacccagata ggaagaaccg atgaacagga
    11821 cggtgtccct tatggcggtt tttataccca ggaagaaatt cgcgaaatcg tccgttatgc
    11881 cgcttcaaaa tacatcactg tgattccgga aatcgatatg cccggacata tggttgcagc
    11941 ccttgcagcc tatccgcagc tttcatgcac cggaggcccg tttgaagtgc gcacccaatg
    12001 gggcgtggcc gacgacatcc tttgtgccgg caacgaagat gtttttgatt tcgtacagaa
    12061 tgtgctcctg gaagttatgg atttgttccc ttcaaaatac attcacatcg gaggcgatga
    12121 agccccgaaa gcccgttggc aggcctgtcc caaatgccag gcacgtatga aagaggaagg
    12181 cctgaacaac gagatggaat tacagagtta ttttaccaaa cgcatcgaac gtttcctgaa
    12241 ttcaaagggg cgtaagctga ttggctggga cgaaattctg gaaggcgggc tcgcgcccga
    12301 agcaacggtc atgtcatggc gaggcattca gggaggcatt gatgcagccc ggcaaaacca
    12361 tgacgtcatt atgacccctg tcgactactg ttacctggat tattaccagg ccgatcctgc
    12421 taccgaaccc ctggcaatcg gaggatatac cacactgaaa acagtttatt cctttgagcc
    12481 cactcccccg gtgctgaatg ccgaacaggc aaaacacatc ctcggccctc agggaaacct
    12541 ctggacagaa tacatcgcca ccccggaata cctcatgtac atggcattcc caaggggaat
    12601 cgctattgcc gaaatcggat ggtcgcagaa agccgaccgc aactgggacg acttctcgcg
    12661 acgcctgcaa aaccaactcg accgtttcga catcatgggt atcaattact cgaaagggtc
    12721 ctttaaaacc gacatcacca ccgtccgtga ttccatcctg aaccgtaacc tggtcaaaat
    12781 caccagcgaa gctgcaggtt atacaattca ttacaccacc gacgggaact ctcccgacgc
    12841 gtcctcgccg gtatactcgg cgccttttcc cattgacagg accagtacca taaaggcggt
    12901 actcgtcaaa gacgggatgg tgaagggaac gcctacggag tgcgaaattt tggttcataa
    12961 agcagccggc aagcccgtca ccatcctgaa gccatacagc tttaaatatc cgggaaccgg
    13021 gataaatgcg ctgaccgatg gcctgaaagg cggaaattct ttcaaagtcg gatggcaggg
    13081 atatgaagga tccgacatgg aagtcgtcat cgacctgctg aaagaggaac ctgtcaaaag
    13141 tatcagcact gctttcgtcc aggatgccgc atcctgggta ctgtttcccg ccgaagtaaa
    13201 atattatacc agcaacgatg gcaaaagctg gaatcttgcc gatacgtttg tgacggaacc
    13261 ggcgcccacc aaaggcaaaa ccaatcataa ctacacgtcg gagctcaaag gattcagcgc
    13321 cagatatatc aaagtagcgg caaccaatgt cggcaaactc cccgattggc atgaaaacaa
    13381 aggccagccc tgctggattt tcgccgacga agtaatcgtc aagtgatcat accctttcag
    13441 cctgaacaat gaaacagttt ctgttccttg ccttgctcct tgtcaccacc ggggcctatg
    13501 ccgggcataa atttcctgaa gtgcggcatg tcgttctcat cggcatcgac ggcgtatctg
    13561 ccgaagcttt tcaatattct ccgacgcccg tcatggacaa aatggcccgg gccggctcgc
    13621 tttctttgaa aacgcgaggc gttatgccaa ccgtcagcgc ccccaactgg gcgaccattc
    13681 tttcgggcgc cgggcccgaa cagcatggcg tcacatccaa caactggagc ctgaagaaac
    13741 ccggctttga agctaccgtc catgacgaaa agggatattt cacctcgatc ttcacgctga
    13801 tccggcaaca acgcccgctt gccaatacag ccatgttcta tgactgggat tggcttggca
    13861 cttatgtaaa tcaggatttg attgatacga tgtcgtacct gcctggattc cgggccgtga
    13921 ccgatgccgc gtcatcgtat atcgcccggc aaaagcctta tttcactttt gtatattatg
    13981 gtcaccccga cgaaatcggg catgaaaaag gatttaacac aagtgagtat taccaggcca
    14041 ttacagaaat cgacggcgag atcggccggc ttatcgactc ggtgaaatat gccggcattt
    14101 atgacaatac ggtatttatc atcgtctccg accacggcgg taaaggtcat ggacacggcg
    14161 gcgaaagcat gatcgaactc gaagtgccct ggatgatttc aggcccggga ataaggcaaa
    14221 atgcagtgct tcaggagccg aacgatctgt caaatacggc gcccgttata gctatccttc
    14281 tggggttgca actgccggtt gaatggaccg ggcgccccgt ggcagaagcg tttctcagga
    14341 ataaacaata caataaaaaa aacttcgccg gttatgtgtc aaaaccaatg tgttccgtca
    14401 ataacggcat ctatctggaa ccgcagacgg ccgctctgag ttcgaccatg gaaaatgcgg
    14461 aaatccgtta cacgctcgat ggcaccattc ctgaccgaaa atcgagaaag tacattgagc
    14521 ctgtgattct ggcaagttct gtcaccctca cttcggtcgc ttttagcgaa aactcacgga
    14581 gcgaaccggc tgtcattaag gtcaatgtcg tcaaaggcat tcgnnnnnnn nnnnnnnnnn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnaaaactc acggagcgaa ccggctgtca ttaaggtcaa
    14761 tgtcgtcaaa ggcattcgcg acattaatct tgcagaagcc ccttcttcga agtacccggg
    14821 acaaggcgct cccggactgg tggacggcgt aagaggtcct gacgattatt ttgacggcgc
    14881 ctggatgggt tttgaagaaa aaaacataga tgctacaatc gactttggaa agaaaaggga
    14941 tatcacctcc atcggcatcg aattcctgca acagccaaag tcctggattt tccttccgga
    15001 agtgatcgaa tactatattt cgcccgacgg cgtcaactgg gagcttaccg gcgacctgaa
    15061 gcctgccgat gctgatggca gcctgcaaag cgggccggta accctgaaac gcaacttcga
    15121 tctgatcata acgcgatacc tgcggataaa agtgaagaat atcggggctt gtcccgaagg
    15181 ccatccgggc gccgggcaaa aagcctggct gtttatcagc gaagtcaccg ttgaatgatt
    15241 cccggtatca gcccgaatct ttacctttgt tcaaatttca gcctctatgt catacacaat
    15301 cgttaccggt gcagccggtt ttatcggcag ttgcatggtg aagaaactca atgataacgg
    15361 catctcaaat atcgttgtcg tcgatgattt cagcaatccc gtaaaaaacc gcaacctgga
    15421 agacaaagct ttcgctgcca aagtccaccg cgatgacttt ttcacctgga tggaagaaca
    15481 cgcccgtgaa acggaatgca tataccacct gggcgcccgc accgatacca ccgaattcaa
    15541 ccgggaaatt ttcgataaac taaacctgaa ttacaccaaa aatgtctgga gattgtgttc
    15601 gcggtccggc atacctctca tctatgcttc ctcggccgca acatacgggg ccggagaata
    15661 tggctatacc gaccgccacg atatcgtgga aaggttaaaa ccgctgaacc cttacggcga
    15721 atcgaaaaac gaattcgaca aatggatgtt ggcccaatcg gaaacgcccc cattctgggc
    15781 cgggatgaaa tttttcaacg tatacggccc caacgaatac cataaaggcc ggatggcatc
    15841 cgtcatcctg cattcgttca aacagatatc cgaaaccggc aaagtaaggc tcttccgctc
    15901 acataagcct gaataccagg acgggatgca acttcgtgac tttatcttcg taaaggacgt
    15961 cgtggatatc cttttcagga tgatgcaaaa aaagcccgaa ccggggctct acaatttggg
    16021 aaccggacat gcccgcgcct ttcttcacct gaccagggcc gtattcagcg ccatgaacaa
    16081 accggaaaac atagaattca tcgatacgcc gctggatatc cgtgacaaat accagtattt
    16141 caccgaagcc tgcatggaca aactcctcaa aaacctcggc ccggaactga aattccattc
    16201 actggaggaa ggcgttactg attacgtgca gcaatatttg atgaaaggcg gatactatta
    16261 atccggatat ctgttctttt cgggcagatc tggatggaaa aaaaaagtac tgatgattta
    16321 gcaacccggt tcaaggcttg ctgaccaatt cccggtctgc gggaattggt cagcaataat
    16381 gatacgggca tcttattcct ccgacagttc ctttctcagc tccagcagaa atcgttttat
    16441 atgcctcagc gattcgataa catcttcctt attgcccgtt gatcgtcttt cttctttcag
    16501 tttcgatttg gcgccttcca gggtaaagcc cttttccttc accagataaa atatcttgtg
    16561 aaaattatcc acatccgcct gagtaaaaag cctgttccct ttattgttgc gatggggcct
    16621 gataatatca aactcgtttt cgtaataccg gatcagcgac gccttgacgc caaaaatctc
    16681 agccacttca ccgatgcggt aatacaagcg tccgaccttt tcttttcgca gtgccatcag
    16741 atgttatcca ttgaaaatca ataccaaatc aaatatgatt accataaccc gtttcccggt
    16801 atagctacgc aaactattag tccattgttt gcgaaggctt cgaggacagc tcaatcatgg
    16861 ctgcgtattc tttcgaagtt atatcgttga agaaataata gatcgggttt accggcgtac
    16921 ctctcaggtg tacttcgtaa tggagatgcg gggccgttga tttcccggta ttgcccacgt
    16981 atccgattac ttccccgcgt ttcaccttct gccctttccg cgctttaaac cttgacatgt
    17041 gggcataaaa tgtatcatag ccataaccat ggctgatttt taaagcattt ccgtatccgc
    17101 cgcggttgta ctcggcttca gtaacaacgc cgtcgccggt tgcatagatc ggtgtgccga
    17161 tcgaagccgt aaaatcaata ccctggtgca tttttattac tttatagaac ggatcggtac
    17221 gataaccgaa aaatgaaccg atgcgcctca aatcgctgat atctaccggt tgaatggccg
    17281 gaatcgaggc tatcatcttt tccttggatt ttgccagttt gaaaacctcg tcgtacgatt
    17341 tcgactgcac atacaattga ctcgtgatac cgtcgagctt tttggtggca ttggtgatga
    17401 tttctgaatt attgtaacct tccagcctcg aataccggtt tgcaccgcca tagccggctt
    17461 tacgcaccga tgacgggatc ggttccgttt caaaaattac ccggtaaata ttatcgtccc
    17521 ggtcctgcag atcggagagc accagttgta cctgatccat gcggtcattg agtatcttgt
    17581 actgaagttt gtattgttcc acttcccgct gaagcacctt ttcacgcggc gaagggaaga
    17641 aattgtacag tacgatcagg ccgattcctc caaacaccaa tccaaaagcc agggcgctga
    17701 acaaccgcct gaggcggtct tttaaggtta ccctgacttc ttcaatttta agtgatttgg
    17761 tattaaactg gtatctaact tttaccatct tcagattttt tatgcggtca acagcccgga
    17821 ttttgatcaa atcgaaccgt ttcgggcaaa attaattatt tactgtaaat ttgcatcccc
    17881 ttttcttctt aacatatgtt aaggtgactg tgtataaaat tgccgttcag taatttacac
    17941 gtcaggaacc gggaaacgca attcaggtta taaaaataaa agaaacaatc tataattcaa
    18001 tgacttcagc agaaatacgc cgcactttcc tcgacttttt cgcctcaaaa cagcacaaca
    18061 tcgttccttc agctcccatg gtcattaaaa atgacccgac gctgatgttc accaatgccg
    18121 gcatgaacca gttcaaggat attttcctgg gcatcgcacc ggtcaaatat ccgcgtgtgg
    18181 ccgattcaca gaaatgcctc agggtttccg gcaaacacaa cgacctggaa gaagtgggac
    18241 acgacaccta ccatcatacg atgtttgaaa tgctcggcaa ctggtcgttc ggcgactatt
    18301 ttaaaaaaga agccattgcc tgggcctggg aactgctcac cgtagtttac aaaatcgatc
    18361 cgtcacggtt gtatgtcacc gtttttggcg gcgacgatca cgacaacctg ccccgggaca
    18421 ccgaagccta cgacatctgg aagaaatgga ttgccgaaga ccgcatcctt cccggatcga
    18481 aaaaagataa tttctgggaa atgggcgatt ccgggccttg cggaccctgt tccgaaatcc
    18541 atatcgacat ccgcaatgct gatgaaaggc ttaaaactcc cggacatgag cttgtcaaca
    18601 aggaccatcc tcaggttatt gaaatctgga ataacgtttt catccagtac aaccgtctgg
    18661 caaacggatc gctggtgccg ctgccggcca aacacgttga cacgggcatg ggattcgaac
    18721 gcctttgcat ggtattgcaa ggaaaacaaa gcaattacga taccgacatt tttacaccca
    18781 tcatcggtga aatttccagg ctttcgggta tccggtacgg cgactcggct aaaagcgatg
    18841 tagccatgcg ggtaatcgcc gaccatctgc gcgccgtatc gtttgccatc gccgacggac
    18901 aactgccttc aaacgtaaag gccggttatg tcatcaggcg tatcttgcgc cgggctgtcc
    18961 gctatgccta ttcgttcctc aacgtgcgcg atccgttcat cttccggtta gtcccggtac
    19021 tcgaacaaca gatgagcggc cagtttccgg aactaaaaag ccagtttcag ctcattcaac
    19081 gggttatcca tgaagaagaa acatctttcc tgaggacgct ggctgtcggt attcagaaat
    19141 ttcagcaata tgttcaatca caggaaagct caaaggtgat tgccggcgat tttgcttttg
    19201 aactttacga cacttacggc ttcccaatcg acctgaccca actcatgagc cgggaactcg
    19261 gctggtcggt cgatatggac gatttccagg tccgtctgga ccaacagaaa agccgctcgc
    19321 gcaaagatgc ttctgtggaa accgccgact gggtggaact gatcccctca acaggaagcg
    19381 aattcattgg ttatgatacc acttccgctg aaataaagat cacatcctac cgcaaagtaa
    19441 ttgccaaagg caaggaaata tttcagttgg tatttgataa gacgcctttt tatgccgaaa
    19501 gcggcggaca ggttggcgat accggttata ttgaatcgcc ggatgagcgc attgaaatca
    19561 cagatacaaa aaaagaacac gggctgatcg tgcatctttc cgagaccttg cccgaaaatc
    19621 cggctgccgg gtttcatgcc cgggtcgatg tgaataaacg catcctttcc gccgacaacc
    19681 attcggcaac ccacctcctg cattattcgt tgcgcagcat cctggggacc catgtggaac
    19741 agaaaggatc cttcgtaagc cccgactatc ttcgcttcga cttctcccat ttccagaagc
    19801 ttaccgacga tgaaactgcc agggttgaaa ggatggtcaa caggcttatc cgcgccaata
    19861 tcagcctgga cgaacaccgt tcggtaccga tgaaagaagc tcagaagatg ggcgcccttg
    19921 cattctttgg cgaaaaatac ggcgatgccg tccgtgtaat ccggttcggc gattccgtgg
    19981 aactttgcgg tggcacgcat gtgaaagcta cgggccagat cggcctgttt aaaataaccg
    20041 gtgaaagcgc catcgctgcc ggcgtccgcc gcatcgaagc cattaccgcc gataaagccg
    20101 aagaattttt taatacccag gtcggcgtcg tcgaacaact gaaatcactg gtaaaaaacc
    20161 ccaatgatcc gttgcgcgcc gttcagcaaa tactcgatca gcaggccgaa ctgaagaagc
    20221 agcttgaatc gctgaacgca gaaaaagccg gaaaacttaa agacgaactg gcccggaaag
    20281 cggagcccgt gaacggcatc aatttcattg ccgccaaagt cgatcttgat tcgcaggcta
    20341 ttaaagacct cgctttcgcg ctcaaagcca gggttgaaaa cctgtttctg gtgctgggca
    20401 acgaaagtga aggaaaagcg ggccttacag tgatgatctc cgataacctg gtttctgaac
    20461 gaagacttaa tgcaggacaa atcatccgcg atcttgccaa aaacatccag ggcggtggcg
    20521 gcggccagcc aaactacgca acagccggcg gaaaaaaccc ttccggacta tcatcagcct
    20581 ttgaacaggc aaggaaaata gtcggttaag cgtgttcccg aaaggtcaat ggctttaatt
    20641 ccccgaatca tgcactttcc cggcttcgca acggacagtg cgggaaggga atttatctat
    20701 catgttataa ttgtgcgttg ccataaaaac ggcccgccct gctcttcgta tatcgaaaag
    20761 caactgaaga ataccttcgg atgtttcagg gtcaagatta ccggtaggct catcggccag
    20821 gataagttcc gggtcgttaa tcaatgcacg ggctattgaa acgcgctgct gttcaccgcc
    20881 ggaaagctga tgaggcattt taaacccttt ggtgatcagc cctactttat cgagcacttc
    20941 attcatccgg gaacgcatgg cttcggcatc cttccatccg gtggctttca ttataaaaag
    21001 caggttgtcg ttgacgctgc ggtcgttcag aagctggaaa tcctggaaaa caattcccag
    21061 cttcctgcgc aggtatggca cttcctttct ctttaattcc cttagattat atcccgctac
    21121 ttccccctcg ccttccgtga gaggcaattc agcgtataat gtcttgagaa ggctgctttt
    21181 acccgtaccg gttttcccga taaggtagac aaattcccct cggttaatac aaagagaaac
    21241 atccgacagg atcaggatgt ttttctgaaa aacagacgcg tgggaaaggg agatgattgt
    21301 ttcggtattc attgcaatat ttatctgatg ccaaaaatag gcaatttgag ccaatgcaag
    21361 aatacaggac agtaaacgga gattccttta tcccggcaag gttactttac aagccctgaa
    21421 aatcccatga atgcaaggga taacagtccg gcagtgatca gtgaaagggg gaatcctttc
    21481 atgcccttcg gatagccatt caattcaagt tgctcgcgaa tacctgccat caggatcagc
    21541 gccagggtaa agccggctgc tatggatgcg gcataaacga cgcttgaaat caggttgtag
    21601 ttcttttgaa ctgcgaggat ggcaataccc aaaacggcgc agttggtggt gatcagcgga
    21661 aggtaaattc ccagcgcctg atataatgcc ggagccgctt ttttcaggat aatctctacc
    21721 acctgcacga ggaaagcaat gataaaaata aaggtgatgg tttgcataaa ttcaatatgc
    21781 aaaggcacca ggataaagaa ttgtatcagg taggttacca ggttggcgag cgtcatcaca
    21841 aagataacag ccacccccat acctatcgaa gtgccgactt tattggaaac gcccaggaag
    21901 gggcaaatgc ctaagaattg agccagcact acgttattga cgataagcgc cagtatgact
    21961 atcttgagaa gttccatatt ctaatttctt tcacagatta atgaatgacc aatcaacatg
    22021 cagaatcatc agatcgagta tttgctcttg atcctgttca tcagggcaat caggtatcca
    22081 aaggtgataa aggcgcccgg aggcaggatg aaaataagga tggtgcgggc ttcctgcgaa
    22141 atcaggcgga tatcaaatac cgaaccgtta cccagaattt cccggatagc gcccatgaaa
    22201 gtaatggcca gcgtaaatcc tatccccatg cccagtccgt cgagcaatgc gggcatcacc
    22261 ttatttttct gtgcgaacgc ttcggcacgg ccaagaatga tgcagttcac aacaatcagc
    22321 ggaatgtata tgcctaacgt cttgtaaagg tcaggggtat aggctttcat gaccaggtca
    22381 acaatggtta caaaggtggc gatgatgaca atgaatgccg cgatgcgcac tttatcagga
    22441 atcacattct tcagcccggc aatcatgatg ttggagcaaa ccagaacaaa ggttgtagcc
    22501 gcacccatgc cgatcccgtt aatggctgct gttgtcacag ccagggtagg acaggttccg
    22561 agaaccagca caaaggtcgg gttctccttc cacaacccgt tggtcagata ttttaacatg
    22621 ctcatttttt acctcctttc gatgtagcgc cggaagtggt atctacattg gcttgatttt
    22681 tattcagcat attcatgtaa acattaaatg ctttttgaac accatcgcaa taagcccttg
    22741 aactgatggt agccgctgtg atggcgtcga cagttccacc gtccttcttt actttaagct
    22801 gcttaccggg aatcgttccg atattcatcc cgttgaactg gtcggcgaat tttggttctt
    22861 tcatctttga acccagcccc ggggtttctg aatggttgag tacaacaatt ttattgatgg
    22921 tgccgtcggg tttaaatccg accatcaact caaagtgccc gctgaatccc ttttcggtga
    22981 atgttttaac ggcataacct accggctctc ctttctgggt tgccgtatac agtttgagcc
    23041 cttccatatc cctggcatca gcggtaggat cattgtcaaa cggaggtaaa acatccttta
    23101 cagcctgtat ttccttctgt ttatcagcct gttctatagg atccttggtg atcttatata
    23161 caaatcccaa tgcaccggcc attatcaccg acattaggaa caggcaaaga accatatttt
    23221 tgagtgttga ttctagcttt ttcgacatcg tgttacactc ttaagaattt ctgattaaaa
    23281 aaatttaagc ttttaccgtc tcgccaaagc gttttggctt gatgtatttg ttcagtaacg
    23341 gcgttactgc attcatgatg aggatggcaa aagatacgcc ttcgggatat gatccccaaa
    23401 cgcggattaa aacggtcagc aatccacatc ctaccccgaa gatcagcatc cccttggtag
    23461 tcatcggcga tgtcaccata tccgtagcca tgaagaaagc gcctagtatc aaaccgcctg
    23521 ctaacaggtg aaaaaccgga tcgatatact tatcgggatt aatcaaccag aggatacccg
    23581 tgaagatgaa taccgtcagc aggtacgata ccgggatatg ccaactgata atcttgcgga
    23641 acagcatgta gataccgcca atcagcaggg ctattgccga cacttcaccc aatgatccgc
    23701 caatatttcc cagcagcatg ttgccataat ccggcaattg tgaagttatc tgcgataagg
    23761 tttctccgct tttcatccct tccctcaagg ttccgagagc ggtaggccct gtaagggcat
    23821 ccaccagtac aaagcggttc tggatgggca acggccagga tgtcatatcg accgggaagg
    23881 agatcagcat gaaaacacgt cctgccaaag cgggattaaa cgggtttttc cccagtccgc
    23941 caaacgacat ctttacaaca gcgatagcga atatactgcc gatgaccaac tcccaccaag
    24001 gaatattact cggaacgtta aacgccagta aaacgccggt gataattgcc gaaccgtcgt
    24061 tgattgtggg ttcctgtttc aggacaaatt tcgtaaccag gtattcaacg gcaacgcagc
    24121 taaatacggc aacagccata gatattaatg cccccagccc gaaataccac agcgaaacca
    24181 gcatggccgg aaccaatgcc agtacaacgc cccacatgat cttttttaca ttttgatcgc
    24241 catgaatgtg cggcgaccct gatatggtga gtaactgcat agctctactt agttcgtgaa
    24301 cgaataatct gacctacttt atttttccca agcctgagat aatccagcaa tggacggcct
    24361 gacggacagg taaaaaggca acagccgcat tccatgcaat ccatgatccg gctgttttct
    24421 gcttcttccc acaggccggc atttacctcc cgctcgagca gatatggttc aaggcccatc
    24481 gggcaagccg aaacgcattt accgcagcgg atgcaattcc gcatttccat gcgttttgat
    24541 ttttcttcgg gaaccaaaac aatacctgaa gtgcctttca ttaccggaat gtcggccgaa
    24601 ttgagggctt tacccatcat agggccgcca ctgatgattt tgcccgtttc agcaggcaaa
    24661 cctccggctt tttcgatcag ctcagaaacg ggcgttccga tacgcaccat gaagtttgac
    24721 ggtgaggcga gcttatcgcc ggttaccgta accacccttt caaaaagcgg cttgttcttt
    24781 tgtatggctt cataaacagc aaaagctgta ccgacattgt tgaccacgca acccacctcg
    24841 ataggcagtt tctgcgaagg cacctcccgt ccggtaatgg ctttgatgag ctgcttttcg
    24901 gctccctgcg ggtatttcac tttgagggca acaacctcaa cccctttgaa cgattttgcc
    24961 agttcctgca tgtggctgat ggcatccggc ttgttgttct caataccgat cacggctttt
    25021 gcaacgccaa gcgctttcat gagcagcgta accccaacga gtatctgttc ccccttttca
    25081 agcatcagcc ggtggtcggc cgtgaggtaa ggttcgcatt caacaccgtt aatgatcagt
    25141 atctcggcct tcttcccttc gggaaccatc agtttaacgt gtgaagggaa tgtagcgcct
    25201 cccatcccca cgatgcccat ctcattgatc ttacggatga tttcctgtgc cgaaagatcg
    25261 caatcccgtt taatggtttc agtcctgtcg atggtttcaa gccattcgtc gcccaccgta
    25321 tcgataaaaa cggcctttct gcgatagccg ctcggatcgg taatatcgtc gatctttatt
    25381 actttccccg aaacgcttga atggacattg gccgaaatga acgattcacc tttggcgatc
    25441 agttgtccgg ttttcacttc gcttccttta tccaccaatg gtttggcagg ggctccgagg
    25501 ctttgtccca gggggatata aaccttctgc gacggcggca atacttcgac cgctttgccg
    25561 gccgagattt tgttctcttc aggatgaaca ccgccttttt taaacgtttt cagcatactt
    25621 aatgcctata atattgatct tgttgaaaat acaattattg atccggatgt caggctatta
    25681 cttcttcctg cttttcagcc gattgtttcg gagcccgggg tttgaagtta atctcctgga
    25741 ttgctccggt tggacattcc tcaacacatt tcctgcatag cgtacatttc tcaaaattga
    25801 tgtaagccag attgttatcc agtgttatag cctcaaaatt gcaggcttta acacattttc
    25861 cgcagccgat acaggccact tcgcaattct tccgggctac agctcctttc tcggtgttca
    25921 tgcaacaaac gtaaatgcgt ctgttcttag ggcctttctt acgcaattcg atgattctgc
    25981 gcgggcagga tttcacacag gatccgcagg caacacattt atcctcgtcg ataaccggaa
    26041 gtcccgtggt ttcatcgagg tacatggctc cgaactggca ggatgaaaca caatcgccca
    26101 atccgaggca gccgttcggg caaccgcttt cgccattata cagggcattg gcaaactgac
    26161 agttcaatgc gccgtcgtat tcaacttttt taggagcact ggcccgggaa ccattgcagc
    26221 gtaaaacggc aatcatcggc tccttttcct ccgccgtaag ccccataatg gccgccacct
    26281 gtttcatgac ttcgttcccg cctacaggac acctcatgct ttctatcgtt cctgctttca
    26341 caatgttctc tgccaggttc cggcaaccgg caaaaccgca gccgccacag tttgcgccgg
    26401 gcaaaacgtc tgcaacctcg tcgatgcggg gatcttcaat aaccttaaat ttctgagcta
    26461 cgatgtacag aattgcggct gatgctatcc cgattatgcc taatgtagct accgcaaaca
    26521 gaatggcctg gttcacgtta agcctcctat atttaaatga gtatctttta atcagggtaa
    26581 gcgacaactg aactgcgctc cccggttaga ccgcggcaaa tgtaaatact attgagaaga
    26641 ttgagaaaat atttcctgat tttttaaacc aatttaccaa tctgatttat agtttattat
    26701 gctgaaaaat catacatgaa acgtcacgac ctgttcgaca tatttcaacg ttgacaaggg
    26761 ttttacaggg ttttttagaa aagatttaaa ttatcggtat aatcataggc tgcaccttat
    26821 tgattttcat cgaaaatatc gttgatttga aattttacat cttaggtaat acagaagata
    26881 aagaaatgat ttttaagcag atgaaaaatt cagaaagaaa acacaacggt ccctgcccga
    26941 aggctccgga ttccggttcc tgtttcttac tcatgagccg gatctgtcag atcccggtgc
    27001 ccgttaatac aattcaaaat acggttcctg aatcgcaggc atgtcatatg cctggtttca
    27061 agcatctccg ataaagccga tgctgatatt ccgggcttcc cgcatacata ccaggcaatt
    27121 tcaaaagctt tcggcaattc catgcggcaa tgatgaaaaa tcgtatgcga cgtggccgat
    27181 tcctcccgtt tgcatcgcgt acaccgacgg gcaaaaggcg ttttaccctt gccatatcgc
    27241 gtgtttccgc aatagcggca cgcaaatcca tctttccatt ttatttccgc caatatttga
    27301 aggcaatgtt cctcatcggg aaacgcctcc gcaaactgac ggacatccat tgtcctgaat
    27361 aacccttgca ggtttgtggc ctgtaccatc tttgtattat ctgacatttt gtcggtggca
    27421 taataattgt tgacaaagtt aaaaacaaaa agtgatattt atgcgatata tttaaaatat
    27481 tgcatatctt tgcagccaca aaacacccat tcttaacacc ggatgatcat gaaacattta
    27541 atgcttacct cagccatcgc cctcctgctc acaataacca gttgcggaaa cgggaacacc
    27601 ggatccggtc ttagcgaaaa atccccggaa acacccggca aacccgaagc ggcaaacacc
    27661 ggcataccgg tactatccgg aggagaaccc gtcgttctga ccaaggcttt attccttgaa
    27721 aaagtttatg actttgaaaa aaatcccgat aattgggttt acaaagataa caagccctgc
    27781 atcattgatt tctacgccga ctggtgccgc ccatgccgga tggtagcccc gattatggag
    27841 gagtttgctt cgaaatatga aggaaaaatt accatttata aagttgacac ggacaaggaa
    27901 cgtgaacttg ccggttattt cggcattcgc agtatccctt cggtactatt ttgcccggta
    27961 aaaggcaacc cccaaatgac acaaggcgca ttgccaaaag aagattttca aaaaatcatc
    28021 aatgaattcc ttcttgcgcc agcctctaaa taaagctaaa tctaaaccga aactttaata
    28081 caccctgata aaaatggaac acctgacaaa acaaactttc ctggaaaaaa tattcgatta
    28141 cgaaaagaac caggagtgga aatatgaagg ctccctgcct tgtcttatcg atttctatgc
    28201 cgattggtgc ggcccctgca aaatgatggg ccccattctg gatgaggtat caaaagaata
    28261 cgccggcaaa atagaaattt ataaagtcga cactgaagcc gagccggagc tctccgccgc
    28321 attcggaatc cgcagcattc cctcgctcct gtttgtaccg gccaaaggca agcctcagat
    28381 ggccaccggc gccatgccga aaggttcact agttgaaacc atcaataaac ttttgctgaa
    28441 cggcaaagtc agctaaacgg taacttgtgc cgcgattatc cctaaaggcc ctgtaaaggg
    28501 ctttttttgt taccggagac tcttttttca tttgacagag gaggtcgtga tttgggatgg
    28561 cccgattttg cctgttcaag ccaagcattt aactttgccg catgaacgtc accaaacaat
    28621 tgccccaggt tattctgccc cttgcctgtc tgcctgatat tgagtatttc tgctggctgt
    28681 tccatgcatc gggagtgctg gttgaaattc aggaaacata cccgaaacag acgtgcagga
    28741 accgttattc catcgaaaca gccaatggcg tgctgaatct tgtcgtgccg gtaacaaaac
    28801 ccgacggcaa ccacacctgc accagggata tccttcttga tcataaaagc ccctgggcaa
    28861 gaattcactg gcgtacgatt gaatcggctt ataacaaatc accctatttc ctttattacc
    28921 gcgaagcgtt tgaaagtata tttttggccc cgcccaagtt actggttgat ttcaacctga
    28981 aggttcttga agcatgttgc ggaatcctga aaacagatcc cggcatcata ttaaccgaat
    29041 actaccgtaa aggatatgag aatgccgttg atatgcgtca gttgatcatg cataaaagaa
    29101 aaacggttca tacctggtcg gtacaagaat ttgaacccta tatgcaggtt ttttccgacc
    29161 gccacccctt ctttcccaac ctcagcatca tcgacctcat cttcaacgaa ggtccttcgg
    29221 cagctcatta cctttaccgc cattgccccg ggaataagcc tgttaaacag ccggatattc
    29281 aatcctcaca tgatggatgc tcatcagttt cttcttgatg atcttcttaa tgaccgaaat
    29341 atccttaagg gtaagttcgc ttgaaacaaa ctgcccgttc ttaagctgat cgttgatgat
    29401 gttttcaaca atctcgctta ttttctgctc atccggcgat ttcagcgaac gcgacgccgc
    29461 ttccaccgaa tcggccatca tcaggatgga tgtttctttt gagaagggaa caggccccgg
    29521 atatataaat accgactcat caaccgggtt ggccgatcct tttaacttgt atgcactgta
    29581 aaaatactgc gtgtatcggg ttccgtgatg cgtacggata aagtcaatga tcggttcggg
    29641 caacttatac tgtcttgcct tttctattcc ccggatgaca tggctgataa tgatgcgcgc
    29701 actttcatcg ctggctaatt cgtcgtgcgg attaagcgta ccggtctgat tttctataaa
    29761 atacaacggc atatccatct ttccgatatc gtgatacatg gcgccggtcc tggtgagcaa
    29821 ggtattggcc ccgacagccc tggcggcatc ttcggcaata ttgcttacca tcatcgaatg
    29881 ctggaaggta cccggcgctt taaccgacaa ttctctcagc aatttgctgt gtgtatccga
    29941 caattcgatc agcgaaacgt cggtaaccat gccaaagaca ccctcgaaaa gaaatatcag
    30001 cgggtagctg aacagcgtca gcaaggcgct tattccgaaa agcacgaaat taaaacgata
    30061 aatccctgag agcgaagctt cctgcaacaa ggtcattccg atatagataa cagaataggt
    30121 acagaatatc cacaacgaag tgaaaaagaa ttgtgaacgg cgggtcagat ttgtcaccga
    30181 gataatggta atgatcccgg ctatcagttg catgtacagg aactcgaaac tgttaggaac
    30241 aataaacccg agaataataa tcgtgataat gtggacaaac agcgccagac gggtgtcata
    30301 aaaagttcgg acaatgatcg gaaccaggca taaaggaacg gcattcagcc acgacacatc
    30361 cctgttcacg ataaaggtag ttgccgaaac catgatggtg atcagcgaaa gaagcaaaac
    30421 aatattgtgg ttctggataa agatatcgcg cctgaaagta tgcatgaaca gggcgaaaac
    30481 gataagcgcc accgagatga gaataatcct gcctgccagc gccatcctga aatccgacag
    30541 cctcccgatc ctggtttcat attcgtcccg gtatgattca agtaccctgt atttctcatc
    30601 cgtgaccagt tccccgttcg aaatgatctt ttcacccttc tgcaccatac cccgggttac
    30661 cgatatattg cgtaacgcct gttcgcgctg ttcgcggttc agggcttcgc tgaacagcac
    30721 attgtgcgca atggattcct gtaaaagctg aagcgtcagg gcgctgttat caggtggggc
    30781 tgaagccagt tgcctggaaa tgtatgcgtt ggcggttctt acagtaaaaa aatggttgag
    30841 cggaaagatc gtagctacat tgccctgaag caatgctatt ttaaaatcgc cgtctttcgt
    30901 ttccaggtcg tccgtcattt cgataatacc atgcgaaaac aaagtgtcga ttatttttaa
    30961 cccggacgat tgcatttcgt taagtgttgc ctgatccgaa tttccatgtt tttgcttcca
    31021 ttcttccgaa agcctctgga taaaacgctc gcgctggctt tcggcaacat cccggtcaaa
    31081 ccggaagtac aggtaggcgt tgttcagaat ctcgcgcctt tcctgttgta attccgattc
    31141 tgatttccgg atggcaaaat cgaaaggcgc tattaattct tcgtgctgcc agggctttcc
    31201 tttctggaat tcggatttta atttaccctg ccgcggaaaa atactgacca gtacggcaag
    31261 cgccgccagg aacaacagcg ctttattcac ttcatagtaa tatttccccg atccgttaat
    31321 cctgaacttc atgatgattc acaaattttc acccacgaat atagataaat ttgacagttt
    31381 agcgccaaag agtaaatttg cttacaaatt attcattttt atattgaaac cctatgaccg
    31441 gaaaatgccc tttaagcctt gtcccgttaa ggcttgagcc cagcgatcgt actgaaatgg
    31501 tcagccaact gctttttggt gaaacatacc atatagtgga agagctcaaa ggatggctcc
    31561 ttgtgaaagt tctgcacgac ggatatgagg gatggattga cattaaacaa tcaatgccgg
    31621 ccgagtacaa ccaggctgtt gaaccggaag taaaaaccgt acatgttgtg gccggcattt
    31681 cggatcctgc ttttaaaagc ggcgatgaca agcctttgat actgtgcagg ggaagtgtgc
    31741 tgcctcagtt ccgcaaaggc aaatttatgc tgggcgggca ggaataccgt ttcgatgccg
    31801 aaaccagcat tattcccgaa gagccctgtc ccgaagctat cgtcgcaacc tcgctggcct
    31861 acctcaattc accatatctg tggggcggcc gcagtccgtt cggtatcgac tgctccggtt
    31921 tcacgcaaat ggtgttgcgg ctatgcggca ttccgctcaa acgcgatgcc tctttacagg
    31981 ctcaacaggg tgaaatcgtc aattttgtca atgaagcgaa acccggcgac cttgcttttt
    32041 ttgaaaatgc tgtaggaaat attattcata caggaatcct gacaggcgat ggaaaaataa
    32101 tccatgcttc aggatgggtg cggatcgaca gcatcgacca caatggtatt tttaatgccg
    32161 aaaccggaaa atacacgcac acgctgagaa tcatccggcg tatggcctga cccgtgacaa
    32221 caactcagaa ggtgtacctg agcctgtccg ttcagatact gattttcatg ggccggaatg
    32281 cctcccctgt tatccgggat ggtgataatt tgataaaaaa tgttgataaa tgttattagt
    32341 gttttaattg gaatttaagt ctctattcct accttaggca ccatctaaga ataatcacct
    32401 aaccattcat tttatgaaaa ttagaaaact tttactacta acttttacct tgttctggac
    32461 agcaatgctg tcggcacagg taaccacatc gggcattaat ggccggataa caaacgacaa
    32521 gggagagact cttcccggag ctacaatagt tgctgttcat attccttcta ataccatgta
    32581 tggcggaatc agcaacgata aaggctggta ttccatacaa ggtatgagag taggtggccc
    32641 gtacaaaatt actgtctcct ttgtcggatt tgccagtacc gagtttgaaa atgttcagct
    32701 ttctctgggt gaatcatcaa actacaatgc gtctttaaaa gaatcatcta gtcagttgtc
    32761 cggagttgtt atcagtgcag tcgtccggaa aaacacatct ggcggggcag ccagcaactt
    32821 caaacaaacc cagattgcca gcacgcctac ggtagatcgc aatatttatg atattgccaa
    32881 attatccccg ctggtttcaa cttctaaagc cgggggtatt tctattgccg gttccaacaa
    32941 ccgttataac tcattccaga tcgacggtat ggtcagtaac gatgtattcg gtcttgccgg
    33001 ttcgggtacc aatggcgggc agactggagc caaccctatt tctcttgatg caattcagga
    33061 agtccaggta cttatctccc cctttgatgt acgccaggga ggatttaccg gcggaggcat
    33121 taatgccatt accaaatcag gaaccaatca gtttcatggc tcggcttatg gctattttac
    33181 aaaccaggat atgtacggaa aatggagcca ggtcaaagaa gaagaagtta aattagctga
    33241 agaatcaacc aaatcctatg gtttttctgt cggtggcccc attatcaagg ataagctctt
    33301 tttctttgcc aataccgaat ataaatttga cacctatcct tctgactatt atgcaggata
    33361 taaacaagat tacatatctg ccgatgaagc tcaggtaatt actgacagat attcagacat
    33421 caccggattt tccgacagct atggaaggcg taacctggaa acaaaaggat tgtctatatt
    33481 gggccgtctc gattggaata ttaataaaaa caataaattc tcgctccgtt accagtataa
    33541 tgattcctac aaagatgtat atggcgccag tgcaaatact tactatttcg ccaacagcgg
    33601 ctaccggata attgataaaa ccaattcgct ggttgcagaa ttaaactctc acatcagcaa
    33661 ttccttatac aatgaagccc gtgtaggtct taccatggtt cgcgacgatc gtgaggtggc
    33721 ttatcaggga ccaaacatta ccatcgaaaa cctgggaggt gtattaactg atacggctgg
    33781 ttggactcaa ggcccgacga tggcctatat cggaacggaa tactcatccg gagccaacac
    33841 actgaatcag gatatctata ccattgaaga caacctctcc tggtacaaag ggaatcatac
    33901 cgttactttt ggcacccaca acgaatttta caatatgtac aaccttttca ttcaggccag
    33961 taacggtgct tattactacc actcgctgaa tgatttcctt aatgataatg cttacaagtt
    34021 cggttacaac tattctgatt atgacctgac aggttcatat aagtgggctg ctaccgtaaa
    34081 agcgggacaa cttggatttt atgcccagga caaatggagt atttcaaaca accttgatct
    34141 cacctatggc ttacgtctgg atattccgac attcttcaat aaaccttcta ccaatgctga
    34201 gttcaacgaa agcaattatg ccacggcata cgacgtaaaa accggtgacg ttcctgcaac
    34261 aacggtaatg gcatcgcccc gtcttggttt ccgttggtac gcaaatgaca gccacaagac
    34321 tttggttcgc ggcggtaccg gattatttac cggtcgtgta ccgtttgtat ggctgtcaaa
    34381 taactggagc aataccggga ttgaaatgaa gggtactacc attacaagca atgttccttc
    34441 ctttgcaaca tttggcaatg atccggttgc agccatgaat tcggcctcag gttctgcttc
    34501 gaaaccgacg atcaacactg tatcaaaaga ttttaaatac ccccaggtat tccgttcgag
    34561 ccttgctctg gaacaagtgt tgccgggaga tgtaaaagta accctcgaag gtttgtattc
    34621 aaaaacgctg aacaacgttt ggtgggaaaa tctagccctt accgataccc ttggcaaaaa
    34681 agtttatgcc gtcagcgctg atgttgaaaa ttcagcagcc acttactggt caagaaatac
    34741 cggaagttac tatgccatca ttaacctgaa gaataccaat aaaggttata cgtatgcctt
    34801 aagtgcaaaa ctcgaaaaaa cctttgacta tggacttgac cttatggcaa gttataccta
    34861 tggccatgct ttttccgtaa acgacgggac cagttctgtg gcttattcca actggaagta
    34921 taactacgct attaatccga ataatgccga tgagctttca tactcattgt tcgatgttcc
    34981 taaccgcatt ctggctgccg tcacttatac ctcaccgaaa tatttaaaca aaaggctaaa
    35041 cactgtcgtc tcccttgttt ataatggatc taatggtcag cgttattgtt tgaccatgaa
    35101 cgaaagcaaa gatttcaacg gcgatggcca gtatggcaac tccttgcttt acatcccgac
    35161 aaaagatgaa cttgcaaaaa tgacctttac cagcgaagaa gatcgtgcag aatatgaaac
    35221 ctggattgaa ggtgacaaat acgccaagga taaccgcgga caatttgctg aacgctattc
    35281 aaacagcgct ccctgggaaa accattttga ccttcacctg gccgaagatt tctactatat
    35341 gagtggtaaa gacggcaaag tatcattgac agtcgacatt ctgaacattg gcaaccttct
    35401 caataaagag tggggcgcta actattcatc atactacaat gtacaaatcc tgaaagtaac
    35461 ctcattaaca acggatgaga atggaaataa gattcctact tactcatacc agggcagcga
    35521 agttaccaag agcgatttct atagccgctg gcacgcccag gttggcctga gagtaacttt
    35581 ctaggtaatt gaacaacagc cagatcaatt aaaaataaaa ctgccggcaa aaaccggcag
    35641 tttttatttt atcccttctt gtgcctccaa tccccccccg tcattggaaa aagaaggggc
    35701 gactttgaaa tatgcaacga tacacaacta aatttgcact gataaatacc gtttaaaaca
    35761 acagattgag ttccggaaca ataaagccgg ttcaaaataa aaacgcgtca tgaaatattt
    35821 catcataacc ggcacatccc gcggaatcgg tgaagctatt gcaagaaagt tgctgatgac
    35881 cggcaattcc cttatttgta cttcccgttc gctgaatgag gatctaacgg aaacggcgtc
    35941 gaacatgagt atcccgttgt actaccatcc gtgcgacctc agccggacgt catcagccat
    36001 ggaattcata aaaaccgcat tccgcattat cgacctgaaa aacgccgaaa gcatcgctct
    36061 tatcaacaac gccggaatgc tggcacccat agggccggct ggcaacctga aacccctgga
    36121 tattgaacgg cattacaaaa ccaatatcct ggcgccggtt aacctgacca acgaatttat
    36181 taaacgcact caaaccctga ccctgcaaaa agtcatactg aacatttctt ccggtgcatc
    36241 gggccaccct tatcatggct ggtcggtata ttgttcttca aaagcggccc ttgaaatgtt
    36301 tacccggacg gttgcactgg agcaggagac agaaaaacat cctgtaatag ttttcgcact
    36361 caagccgggg atcgtggata catccatgca aagcctgatc cgccgatccg atccggaatt
    36421 gttccgcgac agggataaat ttgtaaaatt gcatgataac ggacttctcg tgaaacctga
    36481 aactgtggct gacgccatcg tttcagctat gctttccgga aatatccccc agggcgggat
    36541 cctgaccctg gacgacctga tcaggatatc aagcttgtaa gacgtcccaa aatgacagcc
    36601 atcaacaaaa tttaccgatt atgcttcagg cattaaacca attcgacaca tcgctgcttc
    36661 tttaccttaa cagcttgcac agcccctttt tcgatcatgt gatgtggctg gccagcgata
    36721 aattcatttg ggcgcctctt tatatctggt tcctgtggtt actttaccgg gaatacccca
    36781 aacgcttctg gctgatacta gcgtcggtgg ctttgatgat cctggcggga gaccaattgt
    36841 cgcggctgtt taaagacatg gtagggcgat atcgccccac ccatgaaccg gctattcagc
    36901 aactggtaca taccgtgaac ggatacacgg gaggcgccta cagctttttc tccggacatg
    36961 ctacaaattc ctttgccgtt gcattgttta tgatcatgat gctgccggaa aaacgccggt
    37021 atatgcttcc gttaaccctg gcttatgcgg tcctggtttc ctacagccgc atctacttag
    37081 gagtccatta tcccattgat attcttaccg gagctgtggc aggatcgttc attggctggg
    37141 gcgcatcgaa agcataccgc tataccgacc tcaggctcag caggaaataa gcactacggg
    37201 tcagcagttt tcacctcacg taaccgaata ctttccccga aaaccttttt acctttattt
    37261 tataggtgca cacctcgcga agtgaaggcg gattatacct gaattccctg tcggtataat
    37321 tggacatgat gatattcagt atctcaactt tttgattgtc atcttcaaca aactccacat
    37381 ccccgaacgc ctggacgctg cgataatgca tgctgtagct gcatgcaacc tgttcgcttt
    37441 gatgatgtaa ctggtggtcg gcgctgaata ccacacatac gccgggattt tttttaagta
    37501 tgtcaatttt catccccttc ctggaagaat gaagataaat taccccatcc ttatatccga
    37561 aattgaaagg taacacatag ggcatgccgt tttcgtctgc cattccgaca taacatacct
    37621 ggcatttttc gataatctcg tcaatttcac gttgcagcgt gatgaatttt gctcgcatgg
    37681 ttttcaggtt atgtggtgaa cgtcttattt attgaaggca ccatcaggat tccttctttt
    37741 cgccggctaa ggtttcgagt atccaggttg tcagcgtcac gatgatactg aagatgagag
    37801 ccgtcataaa accatcaacg gcaaaaccag ccagcagttt atcggcaatc aggataataa
    37861 aagcattgat caccaggagg aataaaccga aagaaaagac ggtaaaagga attgtcatca
    37921 ggaccagcaa aggcttcaga aaggcattca gcaatcccag gactatggcc agcaataccg
    37981 ccgtcgggaa accattcaga tgtaccccct tcagaagata ggcagcaaaa aaaacggcca
    38041 gcgatgagac cagaatcctg aggataaact tcatggctta ggatttataa gacaaagtta
    38101 actgaaataa ggaaatcgag aaaccattcc gatttttgcg gcagataccc gatgcgcgta
    38161 cccaggtcaa aaggaaaaag gaaacggaat aaatacacat cggtagtgag ttccgcgccg
    38221 gtcgactcgt acctgtcaaa tctgccggca tcgccggcgt atgtcagatc ataaaacagg
    38281 gcgcccttaa cccgtttaaa gtaggcaaca tcccctgcag agtaatcagg gtacagaaac
    38341 gggaatttat aggtcagacc ggcgctgtac atcctgttat ttttgagggc attgtatcct
    38401 cgcggagaat tcaccaggtt ggaataggaa taatctccct cctgccggta ttgcattccg
    38461 gcatacaccc tgagaccatg atgcttcaaa atgccgggaa agaataaaaa tgtttcggca
    38521 gatgtaatat tgcccagttc gttagcgcca aacggcgaat ggcgcagatt gaggtcaatt
    38581 gcctgtcccc agcggggata gatatccctg gaagcctgtt ttatgtaatg gtaagcatat
    38641 atccggtaat cgatggtatt gattgtgcct gaaattctcc cggaatctgc cgatgtattg
    38701 tgggttatgt tggtccaggt ggtacgcacc gaaggctgaa gcccgctgta atactttccg
    38761 tgtatgaaac aaagagggat tttcacgcct ccgttcaggg atgtttcacg ccaggtgtag
    38821 cggtggcttt tcccctcatc cgactcctcg tatgcagccc gcttcccttc cgttacgctc
    38881 acatcgagaa ccgggtacca accggcatac tcaaaattca gccggtaacg gccgacgcct
    38941 tcggcaatat cccagtcgta acctgccaat gtagttgccg tacttagctc attttgcgaa
    39001 gcaaccgaaa cgcctgaatt taaataggac gaattgaaat cgatgaaagc cggcgcccaa
    39061 ctgtgaaacc tgaacaagtg acacgccttt ttatatggct cggaatcgaa aaccctgttg
    39121 cctattgaag atgaatcaac gactccggat tcctgcggca agagccattt gtacaacgac
    39181 accgaattgt cctgcacctc ctcaaggggc ttccatccgg ccggatcaaa agctgattct
    39241 accggatgat aaccgcccgc agaatactcg gagtaaacaa tcctgttaag atgcggatta
    39301 aaggatgcgt tacaggcgcc aaattctgcc gatgtaaccc tgaaacggtt tccggaagca
    39361 gtatctaccg cataaatatt ctcgatcccg gaataagaac cgttgtacag tatatatccc
    39421 ccggcaatca taggatttgc gatatcggta aagcccggtt tcacaatagt gcgggaggta
    39481 acggtattta cattcagcat cctgatactt ttcccgctgt tgttcagcac tgtaaaaaca
    39541 agcatatgcc cgtcaggcga ccaggaaggt gtcatataaa actcgtcggg cgatgaacag
    39601 agcactttca taatatcgcc cgtcagcata tccaggatta caacagaaca tatattatgg
    39661 tttgtaaccc tgacagcagc catggatttt ccgtccggtg aaatggaagg agcaaaaagc
    39721 ctgctcctgt cagtcaactg acgggtttta cccgacttaa aatcgaacac tttgatgacg
    39781 gaataatttc tttgctgcca tcgggggtct gcgcttttct cggtccatgc caacaccccc
    39841 ccgaccaatg agatattgtc ggcagtgaaa gaacccggct tattcagact gtaacccgtt
    39901 ccaaaaccgc tattgacagt gaagtagtcc gctgagaaga aaccgggtgt aacaataatt
    39961 ttctccttcc cggccctgtc gatcaggaca aacctgctga tgtcatcgag ggtagtcctt
    40021 tccgcgataa aaacactgtc gttaacatac tggggatact tgtataatga ataggttttc
    40081 gctgttttta cgggcagggg acttctttcc gaaagtactg tttttgattg ttgtatgcgc
    40141 cacaggctgt ccaggtaagt gagcgtctgc ccatacaatg cttttttacc catgcctgtc
    40201 accgatttca gcgaccggtc gaaaggaaca atggtatatg gtttgcgcgc tacattggaa
    40261 agaaccattt cccatgaccg ggcgccatat agtctccggg tatttgctac gatctgatag
    40321 cccagttcat actgattggg aacataatct ttatacgacc cgaatacagc tttatcatac
    40381 gtatagattc ctttttgcat aacctgggcc cgtattgcct gttcaaagga agggaccctc
    40441 ccccttcccg aatggctgat ggccgtttcc actgatacag catccccttc catgaaccag
    40501 gtcgggataa aaaggccaag gacagcagca agagcctgtt ccccggttat ttttgacagg
    40561 atgcgggtaa atccctgatc aagcatactc acttggatgg catggcggta ttcgtgaatt
    40621 gccagttgtt ccatccagtc ctgtgcatag gtttgctgcg aagggcacat atacatctcg
    40681 atgcgttttg gagcccatac ggtgaaagcg tttggctcga ccgttgaagt atgaaggatt
    40741 acaggtattg acgaaggcgg ccttgttaac gaaaaggtat ccaatgggaa tgtacattcc
    40801 aggatatttg ccattcgctg agccttggat tcaaactcgc cgggataaac cagcctgaag
    40861 tttttcagcc ggatttgcct ccactggatg gagcccggat cctggcctaa gttataatat
    40921 tgtgcatttg caacctgaca cgatatgaga acctgtatga ttaagaacca cttgaaacaa
    40981 ggttttttct tcgggcagac gaatactttc ataagcgaaa agtgttggac aaatgtatta
    41041 aatatgtgcc ggcagcgcat caaaaaaccc caccttgaga tggggttaac ctgcaatgaa
    41101 aaaaatgata tagtaaaaaa agtagttttt aaaccaggtt gtgctggtaa aatagcgtgt
    41161 taaattcttc gaggctgaag ggtttacggg cgtaaccatc tacaccggct tcaatacatt
    41221 cagctttatt ggcgtttgtg gtaatcgcga ttatggttgt atgttctttt tgctgattgt
    41281 cctgttccat gcggcgtatt tcctttacag cctgtaatcc atccatttcc ggcatttgaa
    41341 tatccatgag aataagatca taatgttcat tgctgaactt acgcagggct tcagatccgt
    41401 tacgggcaac atctacatcg tgcccgcatt ttttaaggta gtatgaagtg atcttttcga
    41461 tcatttcatc atcttcagcc aacaggattc ttaatctctt cataatttgt taactagccg
    41521 gttttaaata atgttattcg tgctctgttg atttaatagc agtaaagttt gcatgtctca
    41581 accttctctc cggtttcaat ccgcgttata tatacccggc caggcgaaac cggtattttg
    41641 actgccgcca gggatgttac agccgaccgg aatattcttt tcccgtcaac ggtaaatact
    41701 tcgataatgc cggaacgtcc ttcagcatcc tttatatcaa tggctgaacc tccggccgta
    41761 accgagattg tatttgtttc attttcctga attccgcccg gtaaggcaaa atgcagtttg
    41821 aagtgacgaa caggatctcc gttttcggat tcaaaactgt aaaccgaatc ggcacgaaga
    41881 tccgtgtata tgccggtgct ttcatcttca agatagacgg gatatctgaa ttcaaatgca
    41941 gaagccccct gaacctttat gacagatatt ccggcatacg gtatgataac atctatcggg
    42001 ataattgacc cggaagaaac agatcccagt gtatttatag ccagtttgct tccgtcggcc
    42061 agcgtggtat aaatgcccgg agccgtgttg aagccctgca attcgtatgc atcataagcg
    42121 atatcaaaat cgcctgaggc atccggatta aaccgcacca ctgcttcatc cgaaaaatta
    42181 ttgccttcga ttttgaattt taaggctgac aacggcaatg gggcaacgaa atcaccatct
    42241 tcagcatgga tacgcatctt gttggtcgta cttatttcac ctgaaacacc ggctttcaca
    42301 aaaaagccct gcatcggatg tacaaccgga cttccattgt taatgcagat gggttcctcc
    42361 ccggcgcaga acactgagaa gttgcccgat ccggaaggat cccaataata tatggtactc
    42421 atccccgggg gaaaatctcc gtgatcatat ccattgccaa gcagactcca gtcgatactg
    42481 ctgggatagg gattgcccac caggttccac ccgtcgccat cggaagtgat ggcaatcttt
    42541 tgttcgccgg aaagaggcac ccccgtaaaa ttgcggtaat cttcataggt ggacaacact
    42601 tcgtaaccct gcatcggatt caatggaact gttgttggca cgatatattc gccccatccc
    42661 gatttttgct cgttataatt acggaggtac atattcagga atacccctga ctcggctcct
    42721 gataccggtg aagaaaccag cagattttca ttggccggaa cagaatatcc cgccgagaca
    42781 tttccttcga cgggcccgta agtaatcagt gagccttgtt tatttttact gtcttgaaga
    42841 gtgaacaaag ctcctgcatc aacatgcagc aatccgttaa cggtgacttt gccctccggc
    42901 atcagtagaa aagatgcaga accttttaca gtaaggttta gaacttccag gcggccggaa
    42961 cattcaccgg cagcatcgga gctgatatta accgtacaac caggcgccag cagcgaacct
    43021 tctttttcga taatcagttt gccggtgttc agttcaaaat tattgtacat caatatttca
    43081 tgacggacca tgatcgtatc atctccctgg ggagtaaccg cgccggtggt tccgttttga
    43141 acaatgcgcc aggcggctgg atcgtcccat gacaggtgat tcccggcaga acagattttt
    43201 aagcctgcac ggctgaaacc tgtcaagccc gagcaaagca gaaaaaccaa aagggtccat
    43261 gtcagcagcc tgtatttcat tctcaaaagt atgcttcaga taaactgttt ttccattttt
    43321 gcattcatgc cccaatttac acgttgcagg acagatccac agatcatccc gcaataaact
    43381 gataacaaat tatttagaaa tcattaattt cccgcctcat tatcggtttc ccatgtatat
    43441 ttgaacgcac cgccatattt aacaaaagat atgcccaaat ttacacgcag ctatttttca
    43501 aacgattaca ccggtattca aaaaaattaa aatgattgaa atgtcctaaa ttggcaaacc
    43561 ttacatcaat tttcgccaat ttgttttccg ttgaaggatc gggtaacacg aatgaccggg
    43621 agcatttggt cccaattaaa taaaaaaaga atctattgct ttggcaaccc tgaattatag
    43681 gcattaacca taatattcca aaccttttca ggatgtggag gggccgttac agaaatattt
    43741 aatttagaaa ccgggtgaat aaaatcaatc tttcttgaat gaagatgtat tgagccatcg
    43801 ggattggagc ggggagagcc gtatttcaga tcgcctttga tagggcatcc gatagcggaa
    43861 agctgcgccc tgatctgatg atggcgcccc gtgtgaagtt caatttccag caaaaaatat
    43921 cgttcactgc tggcaatcag gcgataatgg agttcagcaa cctgcgatcc cttgcgcggg
    43981 ccggcgacaa cataggattt attctgcgct tcattcctga ccaggtgatg aatgagcaat
    44041 ccttccccgg caggaggttt attcccggtc actgcccagt aagttttgtg tatttcatgg
    44101 tttttcagca tcaggttgag ccgggtaagg gctttactgg tgcgggcaaa gatcacggcg
    44161 ccactcacag gccggtcgag acggtggaca acgccaagga aaacttcacc gggtttgtta
    44221 tatttttcct ttatatagcc ctttaacgtt tccgaaagcg gagtatcgcc ggttttatcg
    44281 ccctggacaa tctgcgaggg cattttattg acgatgagaa gatggttatc ctcgtaaagg
    44341 atactattgc tgatcagggt aaaactgtca ggcgaatcag tactgttcgc gttcattcgg
    44401 gaaatccagt gattttacgt cggagatata tgatttcacc gagttgatga tttcctcgct
    44461 gaggttgctg tatcgtctca ggaaccgggg tgaaaactcg gttgtaatgc ccagcatatc
    44521 gtgaaggacg agaacctgcc cgtcaacatc cggaccggcc ccgatgccga ttatcggtat
    44581 tctggctttt gaggctactt cggcccccaa ttttgccggt attttttcca gaactacgga
    44641 aaagcaccct gcttcttcaa gctttttagc atcatcaacc aattttcggg cttccggttc
    44701 ctcagttgca cgcaccacat aggtaccgaa cttatggatt gactgcgggg taagtcccag
    44761 atgtcccatt acgggaattc cggccgataa aatccgttcc actgattcaa taatttccac
    44821 cccaccctcc atcttgacgg catcggctcc ggcttccttc atgatcctga tggcagattg
    44881 cagggcttcg cgtgaatttc cctggtatgt tccgaaaggc atatcaacaa caacgatggc
    44941 cctcgatacg ccacgtatca ccgagcatgc atggtatatc atctgatcca gggttatcgg
    45001 cagcgtactt gtatggcctg ccatgacatt ggaggcggaa tcgccgacca ggataacatc
    45061 aatgcctgcc cgatccagca accttgccat tgaataatcg taagcagtaa gcatggcaat
    45121 tttttcaccc ttgagcttca tttcctggag cacatgcgtt gtgacctttg acactgaact
    45181 gaattttgac atatcaattg ctttttaaga acctgatgta ttctcaaatc agataagaag
    45241 gagcataccg gcatcgcctg cgaattgctg cgatttcggt caattgagga tgcaaaacta
    45301 caaagaaaat atccggacag gcaaaagata atttaagggc tggttcccga ctggtaaaat
    45361 atatataaat ttgccatgct taatcgatta tcacccatga gactttctgc catgaagcgt
    45421 atcccctttc ttattctgct tctccctttc gttttttcac ttgcttcctg cgaaaccgac
    45481 tttgacataa cagccggcta caaagacata actgtagtct atggcatcct cagtcaaaac
    45541 gacagtatcc actatctgcg catcaataaa gcctttctgg gaagcgggaa cgcactggaa
    45601 tactctcata tccccgattc ctcttcctat ggcggcaaca ttgaagtcac actcgaagaa
    45661 gtaaccggcg acggaacagg taacatattg acctttgaca ccattacgct tgccatcaag
    45721 gattccggcg ctttctacta ccccgaccat ctctactaca ttgccaaagg aacattaaac
    45781 ccaacagcaa cctataatct aaaaatagtt aacaaaaaaa acgggaaggt tgtaagctct
    45841 tctactaaac tggtacagga ttttaatatt tcaaatccaa actactggtt aaaaacatta
    45901 aattttaaaa aagcaaatac caccactcaa aagttcgaat gggaatcggc tgagaacggg
    45961 cgtctgtatc agcctgtact ctattacttc tacaaggaaa cttcatcgcc cggagacact
    46021 atcgttcgta aagttgaatg gaaatttcca gctaaaaggt catcatcatt agatggcgga
    46081 aaagcaatgt cggttgagta cctgaatgca gatttttacg cgcgctgtac tgcccagatc
    46141 ccatacgcgg atgcggctgc cgaagaagcc gtgattgccc gtgtcgccga tcacctggaa
    46201 ttgattttta cggttgtcgg cgatgacttc aatacctaca tcgaagtaaa cgaaccaacc
    46261 accggtgtcc tgatggaaaa accggaatac tcaaatatta ccaacggcat cggattgttt
    46321 tcatcacgtt atcagaaaaa gtcggactac atgttaggca aggacaccca acttgacctc
    46381 tacaataata ccaacctgaa gtttgtgagg ccggtaggag attaaagaag aattccggcg
    46441 gcaacagtga accggcaatt gaaaaacggg aatcctgcca ggcagctcat ccctgacaag
    46501 gaaatacctt cctgatcctg attgcctgat gccgcgtgaa gctggttatt cctgagtggt
    46561 gacccggctc ttatcccgac gaataccgcc ctttctcctg aatggtccgt tacgtttcaa
    46621 acaggctgac tttttgtcac catcgccctg acaagccggt atgaataaat gctgttcact
    46681 ggcctgaaga accggccatc gcctctttat agctattgct tatcaataag ttacaattcg
    46741 cttttagata ttatcgcgtt tctcttccgg aagataatcc gataaatatt aaattcagct
    46801 atcatttatg gcacaatgat tgacaatgct ttttgtctca aaagaaattc tgaaaactaa
    46861 caatttatat accgatacta tgggaaagat cattggaata gaccttggaa ctacaaactc
    46921 ctgcgtagcc gttatggaag gcaacgaacc ggttgtcatc cccaacagcg agggacgtcg
    46981 tactactcct tcaattgttg cctttaccga aaacggtgaa cgaaaagtag gcgatcctgc
    47041 caagcgtcag gctatcacaa atccgcataa aacggttttc tccatcaaac gatttatggg
    47101 agaaaccttt gaccgcgtta ccaaagaagt ccagcgggtg ccttataccg ttgttcgcgg
    47161 ggataataat acggcccgcg taaagattga cgaccgcatg tattcgcctc aggaaatctc
    47221 agctattgtt ttacaaaaga tgaagaaaac ggctgaagat taccttggtc aaaccattac
    47281 cgaagccgtt attacggtcc cggcatactt tagcgactca caacggcagg caaccaaaga
    47341 agcaggcgaa attgccgggc ttaccgttaa acgaattatc aatgaaccta ctgctgcagc
    47401 cctggcatac ggcctcgaca aaaagaacaa ggacctgaag gttgctgttt ttgaccttgg
    47461 cggcggaact ttcgatattt caatccttga acttggcgaa ggcgttttcg aagtcaaatc
    47521 aaccaacggg gacacccacc ttggaggcga cgactttgac catcgcatta tcgactggct
    47581 tgccgatgaa tttaaaaaag acgaaggcat cgatctgcat aaagatgcca tggccatgca
    47641 acggctgaaa gaagctgccg aaaaagccaa gatcgagctc tcgagttcca cgcaaacaga
    47701 gatcaacctt ccgtatatca tgccggtcga cggtattccc aagcaccttg tccgtacgct
    47761 tacccgcgct aaatttgaac aactgatcga cgacctggtg cgctccacca tcgaaccttg
    47821 ccgtaaagcc ttatccgatt caggatatac agcctcccag atcgacgaag tcattctggt
    47881 cggtggttcg acccgtgttc ccgtaatcca gaaaacagtg gaagatttct tcggaaaaac
    47941 gccttcaaaa ggtgtaaatc ctgacgaagt ggtagccgtc ggggcagcta tccagggcgg
    48001 agtgcttacc ggtgaagtaa aagacgtgct ccttcttgac gttaccccgc tttcattggg
    48061 catcgaaacg ctcggcggtg taatgacaaa actgatcgaa tcgaatacca ccatacctac
    48121 ccgtaaatcg gaagtattct caacggcaag cgacaaccag ccttcggttg aaatccatat
    48181 tctccaggga gaacggccta tggcatcagg gaataaaagc atcggccggt tccaccttga
    48241 cggcatcccg cctgcgcccc gcggaattcc ccagatcgaa gtaacttttg atatcgacgc
    48301 caacggcatt cttcatgtaa ctgccaagga caagggaacc ggtaaaatgc agcagattcg
    48361 tatcgaagca tcttccgggc tcagcgaagc cgaaattaag cgaatgaagg aagaagcgga
    48421 cgccaatgcc gaatcagacc gcaagatgaa ggaagaagtg gataaaatca acagtgcaga
    48481 cgccctcatc ttccagactg aaaaacaact gaaagaattc ggcgataaac ttcccgccga
    48541 caaaaaagcg cccatcgaat cagcgctggc tgaactgaag gaagctcaca aaaaccataa
    48601 cattgctgct atcgacgatg ccatgaacag gctgaacaca gcctggcagg ctgccagcga
    48661 agagctgtat aaagcatcgc agggtgcgcc ccaggacaat cccggcgccg ggcaacaaca
    48721 gcaacagcaa cagggcggag gatcacaacc cgatcaggaa gtgaccgacg tggattttga
    48781 agaagttaaa taactgatat ttatctcact aaatcagaag aggccatctc taacagagag
    48841 gcctcttttt attaggcaac gattcaagtg atgataaatt aatattaatt cacactattg
    48901 gcaaaaatga caaagcaata tgtaatatat ttgtagtgaa actttacact aaaaaaaaac
    48961 gaatattaat actaatctaa atccatatga gaaaatttac tctgtttgcc gtgacagtta
    49021 cggtattttt gttttttggc tttctaaaag tcggaaacag tcaggtagta attagccaga
    49081 tgtacggcgg aggtggtaat tccggttcca cctatactaa tgactttatt gagattttca
    49141 acaggggaac ttccaatgtg aatctagccg ggtggtccgt tcagtacgcc tcggcaacgg
    49201 gatcgtcctg gcaggtaaca acccttacgg cagtctcatt agcgcctggg cagtattacc
    49261 tgatacagga agccgccggt tcaggaggca gcgttcccct tccaactccc gatgttattg
    49321 gtacaattcc gatgtcggga acaaattgta aagttgccct ctgcaacagt tctctcgtat
    49381 tgactggcgc ttgtcctacc agtggacaga ttatggatct tgttggcaca ggtacagcca
    49441 attgctttga aggttcagca gcaagtgtag ctatgagcaa tactacttct gtgatcagag
    49501 catctgacgg atgtcaggat tctaatgaca actcacttga tttttcgtct tcggctccta
    49561 cgcccagaaa tactctttcg ccaacacata cttgttcaac tacggctgca gcgcttccaa
    49621 cccttgatcc tggaacaggt tattattatt caacacaaaa tgttacaatt acatgtgcta
    49681 cagtaggagc atcaatctat tatactattg atggtactga tcccaataat accgggaatg
    49741 gtacattgta taccagccca gttgccataa gttccactac aacactgaaa gcacgtgctt
    49801 atgctacggg ttacgatcca agttccatcg cctctgcaac atatacattc ccaacagtag
    49861 tcaatactat agcagctctt cgcgctggaa ctccgggtga aatttacgtc tataccggcc
    49921 agggcgttct cacattccag caatcgtacc gtcatcagaa attcatccag gatgccactg
    49981 cagccatcct catcgatgat aatgccggaa aaatcactac cacctatgca cctggagatg
    50041 ccatcacgaa tattacagga accgtgacag aatttaacgg catgatggaa tttacccctg
    50101 aagccgatcc cggcgccccg gcatccacag gaaacaccat cacaccggaa gaagtatccc
    50161 tgggcgacct gttaggcaac tgggaaaatt atgaatcaga actgattaaa gtgcccgatg
    50221 ttacgtttac cagtccgtca ggcaattttg ccaacggcac agtataccct gtgacagata
    50281 ttaacggcac cagcggaaat ttccgcacca ccttctatga tgttgactac atcggcactc
    50341 cggttccatc agcacgcgag gatctcgtca ttattcccaa ctcacgttcc gacggagatt
    50401 atatcacatc ccgcagcctt tctgacttta cctacaactc aagcgacaac attgtgatca
    50461 atgagattat gtacaatccg cccgatgcag gtaacgacac catcgaattt atcgagcttt
    50521 ataacaaagg cgttaccgac gttaacctca acggctggta tttttcacag ggaatcgact
    50581 atgtgtttcc agacacgacc atcagcgcag gcgattattt tgtgatagcc cgcaatgccc
    50641 tttcgtttga ttatactttc gggtttacgc cggcacaatg gacaagcgga atactcaaca
    50701 atttcggcag cgagatcgaa atcaaagacg ctatcggcaa cgtaaaagac tatgttcatt
    50761 atcttcccga tctaccctgg gataccctgg caaacggaca tggccccagc ctggaaatat
    50821 gcgatccgaa cgccgataat tcacttcccg aatcatggtc ggcttccagg gtatttgccg
    50881 ctgtaaatac agccggcgac agtattttcg caacaccgaa cgccaggtgc ggaagcggtg
    50941 caaatctcgt gatcaccgaa atcatgtaca acccgcctga atcaggcacc gacaccctcg
    51001 agttcatcga aatatacaat aacgggaatt ccgtaaacct ggaaggattc accttcaccg
    51061 aaggcgtaac cctcacgttc ccttctgttg acctgccttc cgggaattac ctcgtggtag
    51121 ccgccaatgc agccgctatc cagaacactt tcggagtctc cgccatccaa tggacatcag
    51181 gagccctcag caattccggc gaagcaatta ccctgcagga cccttacggg tacatcatcg
    51241 accaggttac ctacgacgac caactgccct gggattccat tgccgacggc tacgggcctt
    51301 cattaaccct ttgcgatccc aacagcaaca acgcgcttgc cgaaaactgg aaagcctctg
    51361 ttgaactggc tgctgtaaat gctgccggcg acagtgttta cgccacgcca ggctcaggct
    51421 gcgtcaaccc tcccgctttt gccaacttta ccggttatcc taccacctta ctcgaaggag
    51481 aatatgttca gtttaccgac ctttcagcca acaatcccat atcctgggaa tggagtttcc
    51541 ccggcggaac gcctgaatca tcaaccgaac aaaatcctaa catccgctac aacacattcg
    51601 gcgtataccc ggtaacgctc acggctacca atgtgtatgg ttcggggacg cttaccaaaa
    51661 ccgattatat tcatgtggat atcgacggaa ttgcccggat tccttctgac gcagtaagaa
    51721 tttaccccaa ccctgctcac gaaaaagtat atgtaaccaa tccgacggac aaatcaatta
    51781 atatatccgt gttgtcgttg ctgggcagcg aagttgtgtc gataaaatcg gcagcagaaa
    51841 ttataccggt ggatatgagc cggttccccg atgggattta tctggttcgc ctgaccacca
    51901 atgacggcaa aaccataaca acgcagaaac tgatcctgaa ataaaagtaa taacggtaac
    51961 tatgcgaaaa aaactactgg ctttagctgc agccttcctg gctgcagcag gcctttcggc
    52021 ccagaatgca tggatcaacg aaatccatta cgacaacacc ggcaccgacg ccgatgaatt
    52081 tattgaagtg gtcatcgaaa atccgggcag ttacacgctt tctgattttg cggtagattt
    52141 gtacaatggt aatgacggaa aaacatacag ccagaagtta ctgagcgaat ataccgtggg
    52201 agccaccagc ggcagtttca ctttttatta cctgaattat acggctgccg gactgagcat
    52261 acagaatggc gctcccgacg gattgtgcct gagctatcag ggaacggtta tcccggctca
    52321 attcctgagt tatgaaggta cccttactgc tgttgacggg cctgccagtg gcatgacctc
    52381 tgtcgatatc ggggtttctg aagtcaattc acctgtggga cagtcattac aactgtccgg
    52441 aacaggaagc cagtatgccg catttgtctg gcagccccct gcaacggcta ccatgggaca
    52501 ggtaaacaac ggccaggtgc tgggaacgta tacccccgat cctgaacctt ccgcttatcc
    52561 tgcctcattt accgcaacag gtcaaggcct ttcggcactg ttgacctggg ttgacgctac
    52621 cggaacacag ttgccagccg gatatctgat taaagcatca acctcttcag ccatcacacc
    52681 gccggttgat ggcacgcctg aagccgacga tgaagatttg agtgacggta aaggtgcgtg
    52741 gaatgttacc ttcggcgccc agtcgcatac atggaacgga cttgctgcat caacccttta
    52801 ttatttccgg attttccctt acaccaatta tggccagtat atcgactata aaacggacgg
    52861 aacggcacct gccgctgaag ccacaaccca ggccatcgtt aagttttata acttcgacga
    52921 cggacttgag ccctggactc aatacagcgt tatgggcgac cagatatggg tgtatgattc
    52981 aattcacgga attgacggaa gcggatgcgc caaaatgagc ggttattcat ccggaaattt
    53041 cgaaaatgaa gactggctga tttcgccagc catcgacctg accggaaccc atgaagccat
    53101 gttcaggttt ttctccggcg agaattatgg cgatggcagc aacccgatgg tcattcttct
    53161 ctcgacagat tacaccagcg gcaatccggc aaccagcggc acctggatcg acgtgagcca
    53221 gggaataacg ctttcaccgg gatcgtggac atggacgcct tcagagtata ttgacctgtc
    53281 atcctacgat cagccaaatg tccgtattgc cttcaaatac acctccacaa cgaatgacgc
    53341 gccgacctgg gaagttgatg atgttttaat cacagccagc tccggggtcg gaatggaaga
    53401 aacgcccaaa gatgttgcct ttgaagcata tcccaatccc tgcacggaat ggttctttct
    53461 gaatgtcgcc gaagccggtg attattcttt ctccctgatt aactcatccg gaaaaacagt
    53521 tataaaccgt caggttcagg atggcgcaac ccgggttgat gtaaaatcac tgtcgccagg
    53581 cctctacatt ttatcggttt ataaccatca aactggaact ttttccactt cacgcgttgt
    53641 cgtcagataa cgccggtcag cggaaataat accaaagcag cctcttcagg ctgctttttt
    53701 tacgcttgtt taggacaaag cgataccgaa ttattaattt tgaatgtcca atctgccgtt
    53761 tatgctccga aactgtttaa atatcttact gcttatcctt attcccgtga tggttctgcc
    53821 ccagacggcc caaccggttc ggtttgaggc gcctacccgt tcgggaactg atccttttaa
    53881 ccatgttgcc tgcggagacc agggagtatg cctgttttat cccaccatcg aagaaagcgg
    53941 gaaagactct gtttcatggt cattttatct tctcgaccgc aactttcaga aaatatggca
    54001 acaaagaatt cccttgcggg aagatgtcag tttcctgaaa agcatttacc gcaacaattg
    54061 tttatacctg ctgtttcacg ataccaaacg gaatgataaa ggaaacatct cggtattctt
    54121 ttttattcct tcgaaacaaa tcctcaccga acaccacagc gacattccat acaaagcgga
    54181 tattgtggat tttgaagtgc ttaacgatct gattttcatt ggatataacg aaaagaaaag
    54241 caaacccggc ctcatagcat tttcgctgat cgatggccaa aagcgcaatt tcgagatcag
    54301 cgccgaagac gatgcgctat tgctggatgt tgccgtcgac agcatctaca aaggcgtata
    54361 tgctacctat aaggttcaga aaaccgccac acgcaacaac ttgtatatta ataaatatga
    54421 tttgtcatcg gcgctccgca gcaccaccac tttcgagggg gaagtcgaaa aacggatcat
    54481 caattcggct gaatacatac cgctttccga tacgcaggga cttgttgcag gctgctatgg
    54541 ttattccaac cgcggacgcc ggacctatga ctattataat gactactaca attattatta
    54601 ttacaacagc ctttacaacc gtccttatac atacgatgcc aacaaggata acacaccggt
    54661 ttcggacggt tatttttctt cgttggtggg cggaagttcg ccacctgcgt tgaagtatta
    54721 caattttgta gatttcaaca acgccctgaa gataaccagc gatatggata ttctccgtct
    54781 tcgccgccgg aacgaaaaaa aagagaaaac cggactgacg ggagaggata atacggttga
    54841 tgatgacaat aactcgctta actatcgcat tctgatgcat agcatcattg catccgacgg
    54901 aaaattcctg cttctcggcg aaacttatta tccggaatac cacaccatga cccaaatggt
    54961 ttatgattat tacggcaggg cattcccttc aacctattcg gtattcgacg gcttccgtta
    55021 ttgcaacgct ttcctggctg cctttgactc atcaggggtt aaagtgtggg acaacggcat
    55081 ggaaatgcgt gacatcctga cgacttacct taaccacaaa atgaattaca tattcgatgg
    55141 cgacgagatg acgctgtttt ataacgcgaa cggtaaactg gcggtaaaaa ccattaaagg
    55201 ccctgaaacc gttgaacata ccgaatatgt aaccattgcc ccaaaacgtc agaccgatga
    55261 ggttacttct gaataccttg gaacgatgga gcactggtac ggcaagtatt tccttgcctc
    55321 cggctatgaa acaatacgga acaacttcct taccgaaaac aagcgcaatg tattctatct
    55381 caataagatg gcttttgaat aagctattcg ttgaaatgtg ctgatccgga aaaggacgag
    55441 gccggatgag gaacgggaat caacatacat ctgctccgcg attgatcgtc tgctgatcgt
    55501 ttaccgaaga ttcagaaacc cggatatggt gatggccggt agaaagagga tagtacccgc
    55561 acaaaaaaaa gccggaattc tgttattccg gcttttctgc ttatcaagga tacgatctag
    55621 tgctttattt ctgatatttc caccctgcgg tttaacgcca tcccttcggg ggtttcgtta
    55681 gaagcagccg gcttggtttc gccgaagaac gacttttcga cacgcgattt cggaattcca
    55741 attttgatta aataggttac aacataaccg gcacgttttt ccgagagcgc catattatat
    55801 ccttccgact ctcgtgagtc ggtatgcccg gcaacgttta ttacaatacc cggatttgct
    55861 gtgagaaaac cggcaagttc atcaagcgct gctttatatt tatcaattac aacatgttta
    55921 tccgtatcga aataaacaac attgggtaac ccgattttcg cttttggaca tccttcattc
    55981 tcggcaacgc ctgccagccg tgggcattgg tctttattat ccggaatgcc gtcgccgtcg
    56041 gtatcgggac acccgctgta tactaaagta cccggcatat ccggacagct atcgtcaata
    56101 tcagggatac cgtcgccgtc ccgatcgggg caaccttcaa aatcattttt gcctggtaca
    56161 tcagggcaac ggtcatcttt atcggcaata ccgtcgccat ctttatccgg gcatccgttc
    56221 aatgctttaa gcccagcttc cgtggggcaa atgtcgtcct tgtccggtat accgtcgtta
    56281 tcagtgtcag ggcagccttt aaattccacc agcccagcta tatcgggaca ggcgtcgttt
    56341 tcatctatga tgctgtcgcc atctttatcc gggcatccct gaaaaaccag taagcctggt
    56401 gcgtccgggc acaggtcctt cctgtcgatg atcccgtcat catcactgtc tttttggtat
    56461 agccttaatg tgaggcctgc tgaaatatag gtgtaatgat cccggcgatc ggtagaagcc
    56521 agaaaatcga gtttgtcatc gctgaaagta atatggtcgc catattcaag attaaggtcc
    56581 actctggacg acagttttac attcatgcca aatccgtacg gcataatata ttcattggtg
    56641 cggccatctg tttcatagag ctttacatca tccagatcgg taacaagcga gtgataggaa
    56701 gaaatcccgg ggcctccgaa cagataaaaa ttgaacaggc gatcggaatt gtaccgtccg
    56761 aagagctcat tgatgtttaa cgtgaggttc agcgagagat caagaaattg tgaattcagc
    56821 tttctaaaat ggatgtctgt gcgttccgat ttcagatttc cggcaacaaa ttgcccgcgc
    56881 aggccgaata ccgggcttat ctggtaaccg cccagcaggc cggctcccca tcccagcttt
    56941 gaattaaaat aatcgtcttt gttcagatct cctaaatatt cggaggcgcc aatgctaaat
    57001 ccaaagtagc catacccgct gaatacttct gattgagttc gttctttctg cgcaaaagca
    57061 tcagaaggtt ttaatgagat gacagatagg aatataaatc ccgccagaat gcatccaaca
    57121 gcaggtgact ttttcataca gtggctattt gttgaacaag atacgatgaa ccttaagagc
    57181 ataagcaaat atatatatta aatcaataaa ttattcctgc taaataaata tttgaagaag
    57241 tttatcgcgg caggttccaa taccttctga tacagcattg aaacatcaat aaattcttca
    57301 ttcccgcata aaataccggc gcctgctcag taaacaaata ataagttcaa tgattaaaag
    57361 ttcatctgag gaagtaactt attaaacatt tcccataaaa caataccggc agtgaccgat
    57421 atattgaaag aatgcttcgt tccaaactgg gggatttcaa tacactgatc agcctgagcc
    57481 atggcttcat tgcttacgcc gttcacttcg ttcccgaata ccagggccag tttaattccg
    57541 gccggagata taaattcagc caatgaaatg cttccttctg cctgttccac ggcaataatt
    57601 ttgtatcctt ccgatttcag tcccgaaagg gtttcggtca catcggcaga atagctccag
    57661 ctcactgatt cggtggcccc caaagctgtt ttttgaattt cacggttggg cggggtggct
    57721 gaaatgccac atagcactat tttttccacc ctgaatgcat ctgccgtccg gaaaaccgat
    57781 ccgatattat gctgactccg gatattatcc aggacaatta ctaccggcat cttttccagt
    57841 tcatgaaatt tatccacgct tacccggttg agttcgttca tctgaagctt acgcatacat
    57901 gtgttttcca gtttacggcg caaatttaaa acttatcaac accttttagg ccaaaaggcg
    57961 catctcaaaa gaagatttat aaatttgcga cggggatttg taatctaccc aatgataatg
    58021 tcatccgttt gaaaagctta acttttaccc tttattttta gcctagccgg caatcaacgt
    58081 taatcttata cctcttggcc tcttccgaaa acaatatcgt aatgactcct ttgatgaagc
    58141 agcaccatgc catcaaagcc aagtaccccg atgccctgtt attattcagg gtgggcgatt
    58201 tttacgagac cttcgggagc gatgccgtca aagcgtccga gattctggga atcgtcctta
    58261 caaagcgcgc caacggggcc gccgcctttg tagaactggc cggtttcccg caccatgccc
    58321 ttgacactta cctccccaaa ctggttcgcg ccggttaccg ggtagccatc tgcgaccagc
    58381 tcgaagaccc caaaacggcc aagacactcg taaaacgcgg cgtcaccgag cttgtgacgc
    58441 ccggcgtatc ctacaacgat aaaattctcg aaaacaagga aaacaacttc ctggcctgta
    58501 ttcacctgtt gccggcatca tcgggagtat ctttcctcga catctcgacc ggcgaattcc
    58561 tgctcgccca gggttcaaat gattacatcg acaagctctt ccagactttc cgccccagcg
    58621 aggttatcgt ccagaagaat aaacggcagc agtttacgga agtattcggc aaccgttttt
    58681 atctaagcta tttcgaagat tgggtattta cctccgattt cgccagccaa ttgctgaccg
    58741 atcatttcgg cactacctcg ctcaagggtt ttggcgtcag cgaactcacc aacggaatca
    58801 ttgcagccgg agccgtcctg cattacctgt cggaaacgca gcacgacagg gtccggcata
    58861 tcagcaaaat atcccgcatc gaagaagacc attacgtttg gctcgataaa ttcaccatca
    58921 gaaatcttga attactcaac accgctgatg aaaatgccac ctccctcctc tctgtgatcg
    58981 accgcacaat ttcgcccatg ggctcgcggc tgatgaaaag gtggatcctg ctgccgctca
    59041 aggacaagca gcctgtcatc gagcggcacg acatggtgga atgttttatc aggaatgaat
    59101 cctttgcaca aaaaatagaa catgaactga aggtaatcgg cgatctggaa cggctgattt
    59161 ctaaagttgc cgtcgggcgg gtttcgcccc gcgaagtgct gcagatcagg agagcgttgc
    59221 aggccattat ccctgtaaag gaggcctgtg cggcttcgga caacgccgca ttacgccacc
    59281 ttgccgatca gttgaatccc tgcgaactga tttccgaaaa aataggccgc gaaatccatc
    59341 ccgatgcccc ggtatcggtt gttaagggga tggtcatcgc ttcaggcgtt tcggaagaac
    59401 tggacgaatt gcgcaatatc cagtattcag gaaaagacta cctggtacaa atccagcagc
    59461 gcgaatcgga acggaccggc atcagttccc tgaaagttgg cttcaacaat gttttcggct
    59521 attaccttga ggtgacgaat tcgcataagg acaaagtacc cgccgaatgg acgcgcaaac
    59581 aaaccctggt aaatgccgaa aggtatatta ccgaggaact aaaacaatac gaacaaaaga
    59641 tattaagcgc cggtgaacgc attcttgaac ttgaaacgca attgttcaat gaactggtgc
    59701 ttagcctctc cggatatgtt gaacctgtgc aggttaacgc ccaattaatc gcccaactcg
    59761 actgcctgct ttcgtttgcg cgcatcgccc atgaatacag gtatgtaaaa ccggaggtta
    59821 acgacgggcc ggccatcgac atcaagcagg ggcgtcatcc cgtcatagaa cgattgatgc
    59881 cggcaggaga ggaatacgtc gcaaacgatg tactgctgaa caacgaccaa caacagatca
    59941 tcattcttac cggacccaat atgtcgggca aatcggccct gctccggcaa acggctctta
    60001 tcgtgcttat ggcccagatc ggcagctttg tcccggctaa ggccgccaac atcggcatcg
    60061 tcgataagat tttcacccgg gtaggcgcat ccgacaacct ttcgtcaggc gaatctacct
    60121 ttatggtcga aatgaatgaa acggccagta tcctgaacaa catctcggac cggtgcctga
    60181 ttctgctcga tgaaatcgga cggggaacca gcacttacga cggcatttcc atcgcctggg
    60241 ccatgaccga atacctgcac gaacatccgc tttttcgggc caaggttctt ttcgccaccc
    60301 actaccatga actcaacgag atggccgata ctttccaccg gataaaaaac tatcatattg
    60361 cggtaaaaga ggtcggcaac aaggtaatct tccttcgtaa gctgactgcc ggcggcagtg
    60421 aacacagctt cggaatccat gtggcacgta tggcaggaat gccgggcggt gtcctgcgcc
    60481 gtgccgagga actgcttccc ttgcttgaaa aaaagcacag caacgaacaa ctggccggaa
    60541 atgccgccct gaaaaaggca tcaaaagcag gcaacgatcc gtaccagctt agcctgatcc
    60601 agttgaacga tcccctgctg gaacaaatcc gcgacgatat catcaacctg gacatcaaca
    60661 cgctgacgcc ggtggaagct ctgatgaaat taaaccagat aaaaaaaatg ttggaaaaat
    60721 attgagagga ctattacaat ttaaaaatct tgtatatttg caccctcaaa tccaacgaac
    60781 gcggaagtag ctcagttggt agagcacaac cttgccaagg ttggggtcgc gggttcgagt
    60841 cccgttttcc gctcaaaacc tgagacctcc tgtattcttc aatcaggagg ttttttttgc
    60901 ccgaatggtg aaacaggtag ac
//
LOCUS       DAOPYV010000006        60093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_369_length_60093_cov_12.429645, whole genome shotgun sequence.
ACCESSION   DAOPYV010000006 DAOPYV010000000
VERSION     DAOPYV010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 60093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60093
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_369_length_60093_cov_12.429645"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2312
                     /locus_tag="PKI35_01350"
     CDS             <1..2312
                     /locus_tag="PKI35_01350"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNX43050.1"
                     /translation="QPVGDRKGGVVWHTQGSGKSLSMVFFTGKIVLALDNPTIVVITD
                     RNDLDDQLFDTFAASKQLLRQEPVQADDRNQLKDLLKVGSGGVVFTTIQKFQPEEGNV
                     YELLSDRRNIVVIADEAHRTQYGFKAKQIDVKDESGDVVGKKVVYGFAKYMRDALPNA
                     TYLGFTGTPIESTDVNTPAVFGNYVDIYDIAQAVEDGATVRIYYESRLAKVNLSEEGK
                     QLVDELDDELDQEDLTTTQKAKAKWTQLEALVGSENRIKQIAKDIIEHFEQRQAVFDG
                     KGMIVTMSRRIAANLYDEITKLKPEWHSPELNKGAIKVVMTSASSDGPMISKHHTTKE
                     QRRSLSERMKNPDDELKLVIVRDMWLTGFDAPSMHTLYIDKPMKGHTLMQAIARVNRV
                     YKDKPGGLVVDYLGIASDLKKALSFYSESGGKGDPTILQEQAVQLMLEKLEVVSQMFN
                     EFPSSGRVVEDRGGFAYEDYFNAGTSQKLSMILAAEEHILGLEDGKKRYINEVSALSK
                     AFAIAIPHDQAMDAKDEISFFQAVKARLAKFDGTGTGRTDEEIETTIRQVIDKALVSE
                     KVIDVFDAAGIKKPDISILSEDFLLELKGMEHKNVALEVLKKLLNDEIKSRAKKNLVK
                     SKTLMEMLENAIKKYHNKILTAAEVIEELIHLSKDIVEMDNEAKAMGLTEFEYAFYTA
                     VADNDSARELMQQDKLRELAVVLTETIRQNASIDWTIKESVKAKLKVAVKRLLRKYGY
                     PPDMQLLATETVLKQAEMIANEITMSENH"
     gene            2475..3488
                     /locus_tag="PKI35_01355"
     CDS             2475..3488
                     /locus_tag="PKI35_01355"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HNX43051.1"
                     /translation="MLPLSVISSLSRSLIYRGRWLNLLLNEGSYLMSTLLKRPVMAGL
                     PWSASIEPTTSCNLRCPECPSGLRKFSRPTGNLSMEDFRKMIDDLSPHLAYLTLYFQG
                     EPFLNKHFADMVRYAKKQRIYVATSTNGHFLDEETAKSVIRCGLDRLIISIDGTNQET
                     YEKYRRGGNLETVKQGIRNITDEKKRLRVNHPFLELQFLVTRFNEGQAEAIKSFAKEV
                     KADKLTFKSAQVYSFEEGNPMIPLAEKYSRYRQMPGGQWQIKNKLPNRCHRLWHAPVI
                     TWDAKVVPCCFDKDAVYIMGNLQEKSFREIWKSPEYRAFRKKVFSERKEINICTNCTE
                     GLR"
     gene            complement(3492..4055)
                     /gene="frr"
                     /locus_tag="PKI35_01360"
     CDS             complement(3492..4055)
                     /gene="frr"
                     /locus_tag="PKI35_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNX43052.1"
                     /translation="MTEEAKFILEEMNESMANALQHLEKEFHKYRTGKASPQMLEGVK
                     VDYYGTMTPVEQVSNINTPDARLIVVQPWEKSMLAPLAKAIMEANLGFNPQNNGEVLR
                     IAVPPLTEERRRDLVKKAKNEAENAKVTIRNMRRSALEDAKKLEKEGVPEDEIKVLEK
                     EIQNITDQYISKVDKVLEAKEKDIMTV"
     gene            complement(4149..4856)
                     /gene="pyrH"
                     /locus_tag="PKI35_01365"
     CDS             complement(4149..4856)
                     /gene="pyrH"
                     /locus_tag="PKI35_01365"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNX43053.1"
                     /translation="MKYKRILLKLSGESLAGNSGYGFDASMLDLYAGEIKSVVDLGAQ
                     VSVVLGGGNIFRGLKGNNAGIDRIQGDYMGMLATVINSLALQASLQNIGIKARVLSGI
                     AVDPVAERISSPRAIQSMQSGEVCIIAGGTGNPFFTTDSAAALRAVEIHADVLLKGTR
                     VDGVYTADPEKDPKASRFETLKFAEAYDKGLSIMDLTAFTLCRENNMPVIVFDVNKAG
                     NLLKIIRGENVGTLVSN"
     gene            complement(4918..6918)
                     /locus_tag="PKI35_01370"
     CDS             complement(4918..6918)
                     /locus_tag="PKI35_01370"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNX43054.1"
                     /translation="MRKPLLPFFISLFLLIMVPWTLKSQIAYSPVIDSLINLATPGTV
                     SLTVRELSGDTNVLVGGTPQTITSRNYQYNSIQNIAAQYIKEKFESYGLQARYQDYST
                     SGRNIIGWKTGAKYPDKKYIICAHMDDMPSNSFAPGADDNASGVVAVLEAARILSSWP
                     SDYTLVFAAWDEEEIGLVGSKAFSDSAAFNGEQILGVLNFDMIAWDSNNDYKMTIGTN
                     ALSQDLTNDYQDVLRMYTPEINWNYTSIEASDHSSFWHNGYPAILGIEEYPGDFNEYY
                     HTQQDQFSILNIPFFTRMVQASVAGLATLGWNCRLEILHEPVASGDDTNPREAVFNVL
                     SPRPVATGLYQPRLYYKTNGNEYQYLLPVSSANNQFTFEIPGQQPGTAVSYYFAVQDS
                     AGIITGTLPAGGRGINPPGTAAPEVPFTYYIAPVKAVTLCSNTVPVPIPDLGSVYDTI
                     PVDILGGVQDIDVTVNITHTMDKTLNISLISPLGSVVDLSSGNGGNNANYTGTIFDDE
                     ASVSIRMGNAPFIGRFKPEQPLSSFDNTNITGSWVLRVQDSIISHTGTLDNWCITLVY
                     YDMSVTVPEFLQASAFSLGQNFPNPAAVTTTIPFNLERESKISLGLFDLYGRKIASLA
                     EGNYSAGTHFIPLETSGYKPGTYFYRLANGSVSRTKALLIVR"
     gene            complement(6992..10294)
                     /gene="secA"
                     /locus_tag="PKI35_01375"
     CDS             complement(6992..10294)
                     /gene="secA"
                     /locus_tag="PKI35_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNX43055.1"
                     /translation="MLNYIFKMFAGGTKSERDIKAIMPLVEKAKQEYQQIVRLSNDEL
                     RAKTVEFREQIARAIEQEESEIAVLRLRLDTEYEMDIEEKEELYKKIDNLEKESYRKT
                     QDTLMEILPAAFAVMKDTARRFMENETVEVTAQQFDRDLAARRDNVNIAGEKAYWDNR
                     WMAGGNMVTWDMVHYDVQLIGGIVLHQGKISEMATGEGKTLVATLPVYLNALPGKGVH
                     VVTVNDYLAKRDSEWMGVLFEFHGLTVDCIDRHEPNSEDRRKAYLADITYGTNNEFGF
                     DYLRDNMARNPDELVQRPHNYAIVDEVDSVLIDDARTPLIISGPTPRGENQEFETLKP
                     EISRLYNEQRSLVNKLLAESKKLLEQGPDGKINPENEKRGGEQLLRAHRGLPKYTPLI
                     KFLSEPGIKAILLKTENFYMQEQSKNMFIIDDELYFTIDEKNNQIELTEKGIDLITKS
                     YQDPKFYILPDVGAELADLEKSPLPEKERMEKKAEILADYSVKSDRIHTVNQLLKAYA
                     LFEKDVEYVLIDNKVKIVDEQTGRIMEGRRYSDGLHQAIEAKENVKVEAATQTYATIT
                     LQNYFRMYKKLAGMTGTAETEAGEFWDIYKLDVVAIPTNRPVLRDDREDLVYKTKREK
                     FNAVIIEIKDLISKGRPVLVGTTSVETSELLSRMLTRDRIPHNVLNAKLHQKEAQIVA
                     EAGKAGTVTIATNMAGRGTDIKLGPGVKEAGGLAIIGTERHESRRVDRQLRGRSGRQG
                     DPGSSEFFVSLEDDLMRMFGSERIAGIMDRLGLKEGEVIQHSMITKSIERAQKKVEEN
                     NFGIRKRLLEYDDVMNSQREVIYSKRRHALYGERLAVDISNMIFDLAETMVTDNQESG
                     DYENFKLDLLTTFSSECPFTEKEFLAENKEKLTQQLFDSVYKSYRDKSNRIAEHTYPV
                     IRDIYENNPRYLYVAIPITDGKKTLQVTPSLKRAYEDKGKEVILAIEKGITLAIIDDA
                     WKEQLREMDDLRQSVQSASYEQKDPLLIYKFESFNLFKTMLQKVNKDIISFILRANIP
                     VQQPDQVREAQMPSRRSDTRLREERTDVINQRTGENTVTGPVKVEKKVGRNDPCPCGS
                     GKKYKNCHGREE"
     gene            complement(10840..11061)
                     /locus_tag="PKI35_01380"
     CDS             complement(10840..11061)
                     /locus_tag="PKI35_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HNX43056.1"
                     /translation="MYWTLELASKLEDAPWPATKDELLDYCVRSGAPMEVVENLQELE
                     DEGEIYESIEDLWPDYPSKEDFFFHEDEY"
     gene            11613..13241
                     /locus_tag="PKI35_01385"
     CDS             11613..13241
                     /locus_tag="PKI35_01385"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HNX43057.1"
                     /translation="MKTFKNVLFIALLSFLATPLLMVAQNGNGNVATQERRLASFSNI
                     EVGAAVNLFLSQGSAQSVKVEIDENLLDRFETEVDGNTLSITANGVNNATKMNAYVTV
                     TSLKSIEANNAATVTGQTALASDNFSYDGSGATKANFEMNSGEMMIELSGAANAIIKT
                     NVNALKTDISGASDLTLTGTATQHDLEVSGAAHLKAMELVTDNTSAEVTGAGNANVMA
                     KIQLKTDVSGAGNLSYYENGEMRHISAPNEMMKSFSGMENIKSVIIENDNDEEGNTRF
                     EIRDGEDTVNVTLGSDKMIIVSDDTVKVKLGRNEVIVNDEGNVKVKHAKKNKFNGHWT
                     GLEIGINGYLTPDMDFNYPTDYSGLEQNYVKSTNVNLNIYEQNINLIKNKLGLVTGLG
                     FTWNNYRFDAPETRLAKRDGELSVYSDQNPDFSYSKSKLVVDYLTVPLMLEYQTNSHS
                     KVNSFHLGAGVVGGLRIGSHTKYVYDDGSRQKKKNHEDFYLNPFKVDGMVKIGWGVIN
                     LYGTYSLTPLFRSGKEPELYPFSVGICLTDLSDL"
     gene            13478..14221
                     /locus_tag="PKI35_01390"
     CDS             13478..14221
                     /locus_tag="PKI35_01390"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNX43058.1"
                     /translation="MSGHNKWSTIKRKKGALDAKRSKIFSKIIKEITVAVKESGADPN
                     SNPRLRLAIANAKGASMPKDNIQRAISKGADKDAASFVETTFEGYANGGIAIFLEATT
                     DNTQRTISNVRSYFNKFGGNLGTNGSLSFIFDRKGIFAIPQGNLDRDEFEMEMIDAGA
                     EDIQLEDGTFTITTAMEDFGPMMKKLEEMHVEPESAELQRIPKDTTTLDLESAKKVMR
                     LVDIFEEDDDIQAVYHNMTITDELMEALD"
     gene            complement(14367..15566)
                     /locus_tag="PKI35_01395"
     CDS             complement(14367..15566)
                     /locus_tag="PKI35_01395"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNX43059.1"
                     /translation="MKVIVLNCGSSSIKYQLFDMPSAEVAAKGLVDKIGLRGASIKHT
                     RNDGTVLKLEGEILDHQAGIEHVLAILTDKKYGSVGDLREIGAVGHRVVHAGEKFNGS
                     VAITPEVIRALEECIDLAPLHNPPNLEGIRAITNLLPEVPQVGVFDTAFHQTMPEYAF
                     LYGIPYSLYEKYKVRRYGFHGSSHRFVSKRAAEILGKDIKDLKIVSCHLGNGASIAAI
                     DGGKSVDTSMGMTPVEGLVMGTRCGDIDAGAVLYIADKEETSIAFTNTLLNKHSGMLG
                     LSGVSSDMRDVEKAAEEGNKRAKTALDIYSYRIRKYIGSYAAAMGGVDVIIFTGGVGE
                     NGCEVREDCLRGLEFMGLEFSRERNAGLRGKEAVITADDSKVVAMVVPTNEELVIAQD
                     TYEIVTK"
     gene            complement(15598..16572)
                     /locus_tag="PKI35_01400"
     CDS             complement(15598..16572)
                     /locus_tag="PKI35_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNX43060.1"
                     /translation="MAEIIAKLEDFTLSRQEQPKGTIQKVGIIGCGSMGQQIAKTISQ
                     HGFDVVCLDVDDQHVADAMAGITTMLDDEIRQWGMTNSEKRLILSRISGSCNYADLSG
                     CDIVIESVSSKVPGAGVELRRNIFNKIEQFVGSDTVISSNTAVLMISDLAAGLKYPER
                     AVGLHFMAPADKVKVLEVVRGAKTSEAAYDTICRFTHMLEKKPILLQESPGNLTTRMI
                     VIMINEACRMLMEGIASIPDIDETLKMNLGYTYGPFELADRIGLDKVVKWMEGIYNEF
                     GEKAYKTSPVIKKLVRANHFGKSTGQGFYKYDANGKVVGFSVNCLELK"
     gene            16806..17696
                     /locus_tag="PKI35_01405"
     CDS             16806..17696
                     /locus_tag="PKI35_01405"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HNX43061.1"
                     /translation="MLTGIPRYKTNPLISDTTGFFNAYIPYTAEKHQTTNDSLLRYYY
                     YPMPAVTPGRNDTISIHETSSVFAPHQLQPIHKNPLANKPLSADWITGVFMLCLIILA
                     VVKASLPRRLQQIFRSAAQLYYVNQLEREGNLLKESISFALGFLYLTSVSFLLYKLSI
                     LFSPGILAGIKSFFLFSAIFLGCLLYYMLKTVLVSSVGTIFKTPKATHDYLINNLIFS
                     IVTGILLFPATILALYINNPIYVWIACSIFSILLVYKFIRTFMIGLANTKYSMFYLFL
                     YLCTIEILPFLLLFKTVQQF"
     gene            17751..18503
                     /locus_tag="PKI35_01410"
     CDS             17751..18503
                     /locus_tag="PKI35_01410"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNX43062.1"
                     /translation="MKVKNILISQPVPVDLEKSPYAEIIRKYNVNVEFHKFFRVEGVN
                     AREFRDEKVYINEYTAIIFNSKHAVDHFFRIAKEVRIEVPETMKYFCMSESVAYYLQK
                     YVQFRKRKIFHADQDPSQLIDLLKKHKTEKYLIPCSNTYNPELTVLLDATKIEYKTAV
                     LYRTLPDDMRDTVDLAKYDLLVLFSPQGVKSLFYNWPEFKQGEKLIGAFGVNTAQAAT
                     EAGLVVNIPAPTATAPSMAMAIDQFIVKNCKK"
     gene            18642..19046
                     /gene="rnpA"
                     /locus_tag="PKI35_01415"
     CDS             18642..19046
                     /gene="rnpA"
                     /locus_tag="PKI35_01415"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNX43063.1"
                     /translation="MKPVTRHTFRKTEKLVSRKLMNQLFSEGKSFFIPPFKVLWLDTT
                     VDAKSPAQLLISVPRRNFRHSVDRNRIKRLVREAYRIHKHQLYAVLSSQGIQCAIALV
                     MTGRNMPDYKEAEEIIMLILQRLSDEHEKVAG"
     gene            19027..19248
                     /gene="yidD"
                     /locus_tag="PKI35_01420"
     CDS             19027..19248
                     /gene="yidD"
                     /locus_tag="PKI35_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNX43064.1"
                     /translation="MRKLPAKILTGVIRFYQYAISPYLPPSCRYTPTCSAYGIEAIQK
                     YGAVKGGWLTLKRLASCHPWGGSGYDPVP"
     gene            19282..20982
                     /locus_tag="PKI35_01425"
     CDS             19282..20982
                     /locus_tag="PKI35_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX43065.1"
                     /translation="MNFIRNHFAVFALLVILGLSSAGVKAQQSQEFEISKNLDIFVTL
                     YKELNNNYVDDINASELMQTGIDAMLEDLDPYTVYIPESEIEDYRFMTTGEYGGIGSL
                     IHQQNGKIIISEPYENSPAQKSGLKAGDRIIKVNDKTAEGKSVEDVSSILKGQPGTTL
                     KLEIERPGMNKPLNFNITREKIVLDAVPYFGMLNDKIGYIKLSSFTQTASAEVKKALL
                     ELKQNPGMKGLILDLRDNGGGLLTEAVNIVNLFVNKGELVVSTKGKQMDKNKTYRTMV
                     DPVDPNIPLAVLVNGASASASEIVCGALQDLDRAVVIGRNTFGKGLVQNIIPLTYNTQ
                     VKVTVAKYYIPSGRCIQAIDYAHKDGNGKAGHIPDSLITAYKTQHGRTVYDGKGIAPD
                     VELDTTRFSSVTFNLYAKYLIFDFATKYAAEHLQIDSPDQFKITDEIFNDFKKFVAEK
                     GFEFSTPADKDLTDLKASAEEEGCWEDIKNDYEALKTRIENHKSQDIDNKRDEISQLL
                     QLEIVSRYYYQKGRIESSLSIDKDLQESIKIINNSPVYTAILDGTYVKPEKPASGKKI
                     "
     gene            21002..22702
                     /locus_tag="PKI35_01430"
     CDS             21002..22702
                     /locus_tag="PKI35_01430"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNX43066.1"
                     /translation="MKILRQQINWNRDSAYHLVLFLLMASIPLSKYMTSVFQFALTVL
                     WLWHGVNTSYLLNFNNANCLSPVIWGRFIGQSLKEISIAFINKWKDFFRNRPAMLLSS
                     LYVLLLIGVLWTTDLHYALKDLRTKVPLFILPLFLATGPKVNTRTLNLMLAGYCLALF
                     GGTIYQLMLLSNLPVADTRGISAHVSHIRFSLNLVLGIYSLLYLAWKKFFRQTAVNIG
                     LVTLALWFLAFMIYMQYDTGVVLTAIVAIILLFLLAFRQSGIKSRIALLLASFLILAV
                     PLIYISTVIHEFRSVKPVYFLKLDQCTRNGNLYTHDTTNFVVENGVYPGLYICDKELS
                     EAWKKRSSIPYDGYDHKKQLIRSTIIRYMASKNLRKDAEGVTSLTNQDVRAIESGIAN
                     VRSINGFNIKTQIYNYIIAYRNYRYRGDPNSGSLVQRLEYWRTSWLIFRDHPVAGVGT
                     GDLPAAFEKQYEAMHSPLKPEFRRRSHNQYLSTLVAFGVLGFAWYMLVLIYTPVRLKG
                     FKRYYFLVYYIIIMISMITEDTLESQEGATFFAFFISLFLLAWNTEGLQDFSPVRRKK
                     "
     gene            23116..23604
                     /gene="cdd"
                     /locus_tag="PKI35_01435"
     CDS             23116..23604
                     /gene="cdd"
                     /locus_tag="PKI35_01435"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNX43067.1"
                     /translation="MNERLISISYTEFNHIGEMAGTDQILLKKAIEAVHSSYAPYSAF
                     HVGAAVRLEGGDIYTGSNQENSAYPSGICAERVAIFTASSQHPGETIETIAIAAHTDS
                     FELCDPVTPCGSCRQVMAEYQNLSGKPIRVIMKGMSERIWMVEGVENLLPLMFHDDKL
                     KK"
     gene            23804..26149
                     /locus_tag="PKI35_01440"
     CDS             23804..26149
                     /locus_tag="PKI35_01440"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNX43068.1"
                     /translation="MKKFTLVLAILAIAAILPVNSQNVPRTMVVLEIGTGTWCQYCPG
                     AANGADQLVDEGKSVAVIENHNGDSYANTYSNARNSYYALDGYPTAKFDGTVTYVGGQ
                     LCPTANIYSAYLPLYEQRIAVSSPVTVCITGTNSGDNYTVNVALNKVGTINSSNLKLH
                     LVLTESDIQQSWSSCMTELNFVNRLMVPNENGTAVSFASGNFQTFTLNFTKSADWVAA
                     NCELVAFLQDNSTKEIFNANKVPLNDIPATAITSVDFTADVTTGCSPVTVHYTDQSVG
                     ASQYNWTFPGGTPATSTLQNPTVVYNTAGTYDVTLVASDGVCGNAVTKTGYVTIASAP
                     VAPGTPAGTSGLCSVAPIQTYSISSVPNADTYTWSLSPAESGVLTPNGTSCTVDWNDT
                     WYGTAEIKVQATNSCGAGPWSPVRSVTLSEVPGPCAPPTGPTALCMNASDTQYFTTGA
                     TPATYYVWSLQPNEAGTLMQDGLSATIFWNDTFTGEAFIKVEGINGSCEGPYSDPLVV
                     TISDNPAAHNVTGGGTYCAQGGAGVPVGLDGSETGTDYTLYIDGVESTTITGTGSALD
                     FGNQTTAGTYTILGTFTAGGCNSTMTGSAIVASDPQEPLAPANPAGPSVVYITSTPTS
                     DYTTNGGTYATTYSWELTPAVGGTVTGNGTSCTVAWDLGYPGDYTIKVQGVNSCGGGS
                     FSIEYPVTVYRGVGMNDPQETKPVIVTPNPASDIVTIIPSQPLTADITVINSVGKKVI
                     EIHQANLGEGYKLGISKLLPGVYYIRVSNSDISQTMKLVVK"
     gene            complement(26255..26629)
                     /locus_tag="PKI35_01445"
     CDS             complement(26255..26629)
                     /locus_tag="PKI35_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNX43069.1"
                     /translation="MIIKFLKFGVVGFSGVFVDFGFTWLFKEIVKVQKYIANAIGFTV
                     AASSNYFLNRYWTFHSTNPQIITEYGKFFFVSVLGLGLSTLVIWTLNGKFKWNFYVAK
                     LFAIGIVTVWNFFANLYFTFGA"
     gene            complement(26670..28433)
                     /locus_tag="PKI35_01450"
     CDS             complement(26670..28433)
                     /locus_tag="PKI35_01450"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNX43070.1"
                     /translation="MLVMLVLMAAQGFSQDNSLISAESLRLHVGVLAADSLSGRYPGS
                     GGDQKAEKYIADCFSGAGLRMLCNDGRQEFRVVTKVHPGAENTLQLADYSAVLYKDFS
                     PLSFTGNGKLKSPVVFAGFGFDLNLDSLRWNDYAGIDVNGKWVMVLRGDPEPDRPNSL
                     FAGFEKERLKALTAKDKGAAGVLLVSPAGIEARDIPMNPQYDRTTANAGILVVSITRS
                     LADRMLASMNDSVAGLVKRITETHRPSPMYMPVMLDGVAGMELEEAPTANIVAMIPGA
                     DSLLCNEYIVIGAHHDHLGMGGSGSGSRWPDTLAVHNGADDNASGVAGLIELARKLSE
                     NRQKLKRSVIMVAFAGEEMGLLGSKEFVKNPPVPLSRIKAMINLDMIGRLDTLTKAVS
                     VGGTGTAAEMDTILSLLSQNGGLSISRSPDGYGPSDHASFYSENIPVVYFTSGAHEDY
                     HTPADDADKINYSGMASILDFVYSLTVDLSCRDQALTYRETGPKQGEMARRNFKVTLG
                     IIPDVVSTANDGLGVDGVRKGGPAEKAGIRKGDRIVSLNGQPVTNIYDYMARLGLLKP
                     GQVTSAEIVRDGEKIVLIVQL"
     gene            29229..30233
                     /locus_tag="PKI35_01455"
     CDS             29229..30233
                     /locus_tag="PKI35_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNX43071.1"
                     /translation="MLTRLNKQLIFRVILPSAAILIIAGVLLSALEDLPAAGAVLILT
                     GLMAGISGILIVHRILRPVAEVEKAVESLENRKCLSDKAVPVQHDDPLLQSLYHISER
                     IELQEKKIQDEKLRRLRSVIDGQDSERQRLARELHDGLGQSLIAARLRLESCEKPGIS
                     HVRSAVDASKNMIDQAIEEVRRISNALQPAALDEFGLETALRTRVMEMAAIAGIKASF
                     ECNGSIERLAKKSKIYLYRIAQEAITNIIKHARATEMAVTIAREDQLVRLTVADNGKG
                     FMIDPKSYAHRNGIQNMRERASLLSGNFIVESAPDAGTRIIVTIPYTRNDGYDKNTPG
                     "
     gene            30202..30864
                     /locus_tag="PKI35_01460"
     CDS             30202..30864
                     /locus_tag="PKI35_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX43072.1"
                     /translation="MTDTIKILLVDDHHLVRTGIANLLSGEPEIEVAGEAADAGEMLE
                     LLKVREADLAILDIALPDVSGIELARQMAKEFPKVKVIFLSMYTSEEFVFNAINSGAK
                     GYLPKNISRAELLDAVRAVYRGEEYFAESISNTILKSYIKKAKSGNLENLRNESQLSK
                     REVEVLKWFAQGLTNQEIADKLFISIRTVESHKNHIMQRLELKTTVDLIKFAIRNNLV
                     NL"
     gene            30904..31419
                     /locus_tag="PKI35_01465"
     CDS             30904..31419
                     /locus_tag="PKI35_01465"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNX43073.1"
                     /translation="MKKAAILLLLFMPFIVNAQEMNRKGADPKKNNEILLGYCNREGF
                     SAINSNFDSAYRAGYDIYQPDDSTLNLLKPALKDVSVKLILGTWCGDSKEWVPRFFKV
                     MDLAGFNYDNLEMIAVDREKKAGDLNIEDLKVDRVPTFIFYKKGKETGRIIEAPYDLI
                     EKDMLKILRDR"
     gene            31469..32311
                     /gene="panC"
                     /locus_tag="PKI35_01470"
     CDS             31469..32311
                     /gene="panC"
                     /locus_tag="PKI35_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNX43074.1"
                     /translation="MRVFEKIEDLRQYLAGLKESGKTIGFIPTMGALHDGHLQLVRTS
                     ISQNDITTVSIFVNPIQFNNPEDLAGYPRNLSADLEKLESAGCDIVFTPSAEEMYPEP
                     VTNEMDFGQLDKVMEGFFRPGHFRGVGIVVKKLLDITGPHRAYFGEKDYQQLAIIRHM
                     VKSLSMPVEIVPCAIVREADGLALSSRNKRLTPEQRAAAPGIYRALLKAKENYNWFTP
                     DGTESLAITEIEQNPELKAEYATVCDATTLQPFEDWMDAEHAVLCVAVYAGQVRLIDN
                     ILLY"
     gene            32454..32801
                     /locus_tag="PKI35_01475"
     CDS             32454..32801
                     /locus_tag="PKI35_01475"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNX43075.1"
                     /translation="MTIEILKSKIHRATVTQANLNYIGSISIDENLMDAANLIEFEKV
                     QVVNINNGERLETYVIKAERGSGVISLNGAAARKASVGDLVIIAGFAQMEFGEAKKFR
                     PAIVFPDANNQIS"
     gene            32808..33830
                     /locus_tag="PKI35_01480"
     CDS             32808..33830
                     /locus_tag="PKI35_01480"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNX43076.1"
                     /translation="MKNKLKTIARFLLFLGIGIFFIWLFLHYLTPEEKKEIIRSFGDA
                     HYGIIGFAILLGVGAHYLRAVRWRMLLKPLGYETGKLNTFFAVMIGYLANLALPRLGE
                     VSRCVILSRYEKVPFNNSFGTVVTERAIDLVVFLILFFINLALQFNKLYTYIDQKIYQ
                     PIEAKFNLTFSLAGSFTYIAIASLVILAALLFAFRRPLLRIKFINKIWGLIKGFWDGM
                     KSIIYLRKPLLFVIYSLGIWALYLLMAYLVFFSLPETSHLGLDAGLATLVFGSIGIML
                     VQGGIGIYPAIVAETLVLYSVSNIKGYALGWLIWSSQTITIILLGILSFILLPVFNKN
                     VSHEKA"
     gene            33817..34314
                     /gene="rfaE2"
                     /locus_tag="PKI35_01485"
     CDS             33817..34314
                     /gene="rfaE2"
                     /locus_tag="PKI35_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNX43077.1"
                     /translation="MKKLEYVKAKIFARDSVDFSRLLAIMKFQDKKIVFTNGCFDILH
                     LGHIDYLAKAADQGDVLIVGLNTDESVRRIKGNNRPIQDEVSRAMTLASLRFVDYVVF
                     FGEDTPYELIKTVQPHVLVKGADYKPAEIVGYDIVKARKGKVVTLDYLEGYSTTAIEN
                     KILNR"
     gene            complement(34933..37143)
                     /locus_tag="PKI35_01490"
     CDS             complement(34933..37143)
                     /locus_tag="PKI35_01490"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HNX43078.1"
                     /translation="MKKHYTFLRLLFAVLFSLSLSASAFCGIISNDSLYPPVNFRVDS
                     LRALMTWDWPSVELLNEDFEDGDALPAGWTTSSAGNSGWYVTENANSLNFYIGPHSQY
                     AATNDFNAGQQNNGCCDFLVTPAVDLTLTDSAVLTFNTCYTGAFNQAAYIELSTDNGA
                     SWQVMDTLQPAAAPYYETWGRIAIDLSQNAGPGGCASAIIRFHAADNGQQASGWAVDD
                     VNITAYVTTLDHYCLWLNDAFLAISDSNAYQINPESLVFGQQYKVEIAAVYANGVSPH
                     DSLMFTNRCLPYPGNLQASVNDNAVILDWDSPDFGNTGLGPWVMYYLLYCNGTLIDTL
                     SSLDTTYWHLNLDQGMYCYKLSAVYNLEPLGYSGQTGESFAAGPACADIFYCCSLPLI
                     EDWTCGSFDLNQWAHGDNWVIDIIYGNPYPCAKFNSVPELTDYESSLEIFTRSFSLST
                     GTPHLLTVQFDLALDDSLETGTEELTVFYKYNDDNWRTVASFTNQGDFQWTHYSYDFD
                     IPQTANMLYFKVAASGEQSNAIKNWFIDNIELNIKYLLYPVYNLTATPSNANPGAISL
                     EWNCPVDSLNLKSAAGTDDLLSYHIFRRVHTAVPGPWEDIAETTKTWYEDHSLASDCY
                     DYYVMAYYTEGTSAISNIDSLNCIGTGVEMTESPDFRLYPNPARELLYWEAASPVKEI
                     KIADQAGRMLYRETALTGLKGRIVTSALADGIYLICFTGNDGKQTVRKFIVSAR"
     assembly_gap    37582..37681
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            37979..39346
                     /gene="radA"
                     /locus_tag="PKI35_01495"
     CDS             37979..39346
                     /gene="radA"
                     /locus_tag="PKI35_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNX43079.1"
                     /translation="MAKIKTSFFCRNCGAQSSKWMGRCSSCGEWNTFVEEVVQHGDGG
                     AKSGGNFKATESRPKLIHDIAPGNEQRTSTGNGELDRVLGGGLVDGSLVLIGGEPGIG
                     KSTLMLQVALQIKNRRVLYISGEESEQQIRMRAERIGYNGNECFILTETNTQSIFQHL
                     EAIKPGLVVTDSIQTLQTNIIESTAGSISQIRETAAEMQRYAKTTGVPVFLVGHITKD
                     GSLAGPKILEHMVDTVLQFEGDRNYGYRILRAVKNRFGSASELGIFEMRDNGLREVSN
                     PSEILLSQRDQEVSGVAIASTVEGLRPILIEIQALVSSAAYGTPQRSATGFDIRRLNM
                     LLAVLEKRSGFRLGAKDVFLNIAGGIRVDDPAIDLAVISAVLSSNGDIPVDSKTCFAG
                     EVGLSGEIRPVSRIEQRIAEAEKLGYETIVISKYNTKPSKLAARQISVVTVTKVEEVF
                     RLLFR"
     gene            complement(39375..40625)
                     /locus_tag="PKI35_01500"
     CDS             complement(39375..40625)
                     /locus_tag="PKI35_01500"
                     /inference="COORDINATES: protein motif:HMM:NF040309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HNX43080.1"
                     /translation="MRLFIPWERAVTLLIERRSGPQLLCIVTFMTPTMEYTSTYHNLK
                     SQFSLSADEIKFSDSHISCRTFSFHSPGCPTDDFNNQTSTSGIFSEAGESIRENQSFT
                     YPVIFPDSGDKFGQAIVLLHGLNERSWTKYLVWAYFLAEKTGRPVILFPIAYHMNRSP
                     LAWSNPRIMTPLLTERNIHLGHVPMASFANLALSERLSENPVRFVTSGVQSAGDLEQL
                     VLQISAGNHPLFEKGAHIDLFAYSIGAFLAQIVMLANPGNLFTDSRLFIFCGGAFFDQ
                     MDGTSKLIMDKNAFDCLRKYYIQDLGTKSDNHPGFAEYLKSTQLGMAFSAMLSSGKLK
                     AFREGVFSKLKNQISSVVLKNDKVIPAAGTLEALGGGSSIEVMDFPYEYSHEIPFPVG
                     EKGIIPSVDAAFKEVFRRASEFLR"
     gene            40732..41598
                     /locus_tag="PKI35_01505"
     CDS             40732..41598
                     /locus_tag="PKI35_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX43081.1"
                     /translation="MMPDFNSPIPHFLTHKRNIVKLVLFTAAFALVFINVYSPFGVDV
                     WFHVTKWQLLFYSSLVILTGVLVIVISRIIMYYVSRKKDINYWQYLGWVLAEIAFMSL
                     FYTFYVKYILHDRRYFPDLWKASIQNTALVLLLPYSVSWLYFSYLDKKSKLAELAQGQ
                     PVNDTSKQMMPFADEKGVLRFSVKKENLLYLEASDNYVNIYYLNGEKVSRFILRNSLR
                     TLEEELKSTDIVRCHRSYMVNSEKVKVIRRDKDGVRLELDAASAMDIPVTKTYLENVM
                     SAFSHTAPGNKE"
     gene            complement(41869..43509)
                     /locus_tag="PKI35_01510"
     CDS             complement(41869..43509)
                     /locus_tag="PKI35_01510"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX43082.1"
                     /translation="MKTVIHYISLLLLILPAFCSAQYVWKKNYTLPYGSWANEVTETY
                     DKGYLTGGLIRNLQSPYFGYILKTDINGNKLWTKVIDGARETTFGSVNATSDGGFIAG
                     GHYNTQGNDIDSYVIKFNACAEPQWCTILPDNIYHYPTIISRGIFETPDGGFFVHRMF
                     EDFVPDGRIWSIAKLSASGNVEWLNYYVYNNPDWYPGSQLQLRTTLTTDTCLVVCGMV
                     NDTIFHPDPGAQSDMPFWFKVDINGNLLWDRKWNLYWGGTPAEVYAVTEGRNNSYYSG
                     GIMDPGLGHPYVFKIDKDGDSISYFNVYNHPGSIACKPHYIRSFNDTTLLLNTQFGVT
                     YTDNWWSLQLIDTLGNNYKCIYKQEHNIPMMSIKTFDNKIVVLGIDMSGNGAFPYDWI
                     SLSKFNTNLEYDSIYTAPRTYDSLCPHPIVSDTIPVPPNCLVFTALPEVKGTSDILQL
                     KVYPNPASSYTTVEMPEFSLTTTKSKSVNQQQYRPLKGEVELSLINISGKIVNTDVFD
                     ASERNHVVNVSKLPEGMYVLHLTQKGKFVAQGKVMVLR"
     gene            complement(43573..47523)
                     /locus_tag="PKI35_01515"
     CDS             complement(43573..47523)
                     /locus_tag="PKI35_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNX43083.1"
                     /translation="MISLSKLSAALVVSITLISFENLYSQANYQVNGRTITKLNNKYY
                     NIVGDDTLKVIENHFLIVPVIGQEKIAMDYLGSHFTNSITYHDALKFIDFRVSSNQDY
                     VQIVNEIGSLGFVSKLITANYLTNIAQGIPNDPSSSNNGWVIEAMELDAAWEITTGNP
                     QTIIAIIDDGRDYDDIDLNQSEAQGWDFIDDDADPDPMINYPSHGTAMSGCIGARTNN
                     NYGTWGIAGGWNSAPVTKMMLRNQGRDPVTNDLLFDVLGFLEAIEYAIDNQAKIINMS
                     YGAPVEAWINAGGEEYMIQLNNAINTAHEAGIVMFASAGDAGYSYVFYPASHEHVISC
                     GASDYYSARLINSLYPICTSNYGTGAEITLPGDHIMANWGDGNVPYPNHLQFSSGSGT
                     SFSTAFASGIASLMIAVNPCLTPDEIKEILYKSCRVWDELTGFIPSPGWTSELGYGIV
                     DAYTAVTLASYSVTENISDNITWNSNRTRGNIIINSGASLTLDDMELDMIDNTGITVM
                     PGGKLYVNNSVITNSCNSTTHRWKGITVYGDNSISTQSPTYQGLAMLTNATIENAEKA
                     IYIKRTSEYGGGMVWALNSDFLNNDVAIDFERYDYASFSHFTGCTFEVNQDFVGTYSD
                     NSIMVNLRAMYGMRFSSCIFRNVYSDAPIGVGIKSYGSDFTLNGVCSSNVLPCPDEYI
                     KRNQFNNLNRGIYAQYNFAGAKTKITYTDFIGNNRGAYFSNCGYFDILNNDFQIPEYT
                     LNGIDKYGLYLEGCTGYHLENNVFESEIANTTGQIGLYIYNSGTTQNYVYRNTFTRLS
                     RAAVADGNNRASGGATGLCFKCNDFSANGTDIDVLATTPSVYTGISLYQGLPSAGNPS
                     TQKDTLAAGNTFTLSTSAYNIRNLSGNVSNYYFHQYTDPLNIKVKPDPVYNIIRSQND
                     NTTYNKAFICKSWISSGAPAIEDLIAQKEESETGAETAGLQLQVLVDGGSTENLTMDV
                     LGSSPPEALELRDQLLSESPYLSDTVMKTAIMQENVLPNAMVRDVLVENPQAAKNSEI
                     MDMLDQRWNPMPDYMQDEVSAGINVVGGREKLEAVRDGWKQQESYLYNRLVSAYLTDT
                     INPNAPQELKAFLQAEQTPEAGFLLADILMGESNYAAAVQAVTALQGNPALNASQEQQ
                     AADYFTLIGILQGLAADNIQPFGLDSLHAIPLFALYESGDNTACVAARDMLVASGLLS
                     YQEPINDSEVTKSIKAESKTFISKGAESVESLRLLPNPACTYTIAEYNILSKQGNAAL
                     TIYNIGGNIVWKQNIVKANDQLVIDLTGFKPGAYTVTLSCGNKSISSRKLTVSR"
     gene            complement(47920..51123)
                     /gene="carB"
                     /locus_tag="PKI35_01520"
     CDS             complement(47920..51123)
                     /gene="carB"
                     /locus_tag="PKI35_01520"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HNX43084.1"
                     /translation="MKKVLILGSGALKIGEAGEFDYSGSQALKALREEGIETVLINPN
                     IATVQTSEGVADHIYFLPVTPFFIEKVIRKERPEGILLSFGGQTALNCGIELYKTGVL
                     EKYGVEVLGTPIQSIIDTEDREIFAENLRHINIKTPRSIAVTTIEDALKASETLGFPI
                     IVRAAFTLGGQGSGFCNNEEELTKLAVKAFSYSSQILVEESLKGWKEVEYEVVRDRYD
                     NCITVCNMENFDPLGIHTGESIVVAPSQTLTNREYHKLRQLSIEIVRNVGIVGECNVQ
                     YALDPGSEDYRVIEVNARLSRSSALASKATGYPLAFVAAKLALGYGLHELKNAVTRTT
                     TAFFEPALDYVVVKIPRWDLNKFIGVSKHIGSSMKSVGEVMAIGRTFEEAIQKGLRMV
                     GQGTHGFVGNSDIEFPDIRKALAEPTDMRIYSIASAFEAGLTVEEVHNLTKIDAWFLV
                     KLRSVFDVMMQLKACDSIEKIPAGLLLTAKQKGFSDFQLARFVTKHEGNLHAEMLELR
                     RYRKSLGIVPYIKQIDTLAAEYPAQTNYLYLTYNADAHDIVPEDDRKSVIVLGSGAYR
                     IGSSVEFDWCSVNALKTAKDEGYRGIMINFNPETVSTDYDTCDRLYFDELSFERTMDI
                     IELEQPKGVIVSTGGQIANNLAMRLHRQGVPVLGTSPESIDKAENRHKFSSLLDQLDI
                     DQPRWSELSEMEHIYDFVDEVGYPVLIRPSYVLSGAAMNVVSNRQELDYFLTLAARVS
                     REFPVVVTEFVQQAKEIEIDAVADKGEIIAYAISEHIEFAGVHSGDATIVFPPQKMYF
                     ETARRIKRVSRDIARALNISGPFNMQFLAKDNDIRVIECNLRASRSFPFVSKVLKINF
                     IELATQIMLGKTVEKPHKSAFDLDYVGIKAPQFSFSRLHAADPVLGVEMASTGEVGCI
                     GENYYEALLKAMLSVGYRIPEKNILLSTGDARSKVELLRSAQMLAEKGYHLFATPGSA
                     RFLNENGVEAVTLYWPDDEATPNTLEYLRNRKIDLVINIPKNLSKTELNNDYLIRRSA
                     VDFNIPLITNARLASAFIYAICKLKPEEMAVLSWEEY"
     gene            complement(51233..51649)
                     /locus_tag="PKI35_01525"
     CDS             complement(51233..51649)
                     /locus_tag="PKI35_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNX43085.1"
                     /translation="MAIITKKLVNEISYTIVGCAIEVHKTLGPGLLESVYHNCMKDEM
                     RNWNLKFESQVWVPVSYKGKDLGGKFRLDFLVEDLIIVELKAVDNLIPLYQAQLLSYL
                     RLANKPKGLLINFHSENIVSQLIPLVTEKFALLPEK"
     gene            complement(51859..52923)
                     /gene="carA"
                     /locus_tag="PKI35_01530"
     CDS             complement(51859..52923)
                     /gene="carA"
                     /locus_tag="PKI35_01530"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNX43086.1"
                     /translation="MSARLMLEDGTVFTGKSFGDERSVAGEVVFNTSMTGYPESLTDP
                     SYAGQILVATYPLIGNYGVPGIRLTNGLPDFFESERIQVTALIVSDYSEQYSHWSAAQ
                     SLGNWLKEYKIPGLYGIDTRALTKIIREKGAMLGKIVFGDDDIDFIDPNQQNLVDTVS
                     CREVITYGEGRTRILLIDCGVKYNIIRHFVRRGTTVIRVPWDYDYSGVEYDGLFISNG
                     PGNPRMCEATIRHLSASIKQDTPIFGICLGNQLLALASGAETYKLKYGHRSHNQPVLL
                     CGSNRAFITSQNHGFAIDQNTLSDEWEPFFINLNDGTNEGLRNKKKKIFSVQFHPEAS
                     SGPTDTEYLFDEFLGMINNK"
     gene            complement(53102..54049)
                     /locus_tag="PKI35_01535"
     CDS             complement(53102..54049)
                     /locus_tag="PKI35_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX43087.1"
                     /translation="MYRKSLMIMTGMILLAGFTSCVSTKKYNSLATRCKDENTKLTAQ
                     VDELTTEVNELTAQGRKLKNENTELKSDTARLGSAYRVLQGDYSDLDQSFSMLKAQNE
                     GNLEETERIMADLKNAQDNLIVREDNLKQLQAELDAKSLSLRELQSALNRNDSITKAM
                     RKAVADALLGFEGKGLTVYTKNGKVYVSMDEKLLFASGKWDVSPDGVAALKNLAGVLE
                     DNPDISVMIEGHTDNVPYNGTGQVKDNWDLSVMRATAIVKILTANSNIEPKRLIASGR
                     SEYLPVEPNTSAASKAKNRRTEIILTPKLDELLQIIGNN"
     gene            complement(54118..54882)
                     /locus_tag="PKI35_01540"
     CDS             complement(54118..54882)
                     /locus_tag="PKI35_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNX43088.1"
                     /translation="MKIVTYNVNGIRAAIHKGLLGWIHDTSPDVICLQEIKALEADIP
                     AGEIEKMGYHHYWHPAQKKGYSGVGILTKIKPGNICYGIGKREYDGEGRFMQIDMGDI
                     SVISVYHPSGTMGPERQDFKMRWLSYFREYMARLRETHPNLVISGDFNICHKPIDIHD
                     PVRNAASSGFLPEEREWMTGFLEDGFIDTFRYFNTQPHHYTWWSYRAGARPRNLGWRI
                     DYNMVTRSLEKRLKRAAILSMANHSDHCPVLLEMDE"
     gene            complement(55044..55979)
                     /locus_tag="PKI35_01545"
     CDS             complement(55044..55979)
                     /locus_tag="PKI35_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNX43089.1"
                     /translation="MKSQQRKRQVARLSVLSNSLLVVFKLAVGLMIGAVSVISEAIHS
                     AVDLVAALIALFAVKASGKPADEKHTFGHGKFENLSAAIEGFLIFVAAVWIIVEAVKK
                     LMYPAPVEAVQWGVIVMAVSATANFIISNRLFKVGRATDSAALMADGWHLRTDVWTSA
                     GVSLGLLLYILGRYFFPELNLHWVDPVIALGVAALILKAAWELTSESIMALLDSSLPH
                     EEEKLIRSIIESMNPIVLSFHKLKTRKAGADRFIEFHLVVNKDMSVKAAHDVCDDITL
                     RIKKELPNAVVMIHTEPCLDNCGGICRGTCEIFDE"
     gene            56186..57961
                     /locus_tag="PKI35_01550"
     CDS             56186..57961
                     /locus_tag="PKI35_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNX43090.1"
                     /translation="MYNNRLYQKIHSFRLRHISPRTFLVILSVITGAFGGLAAVILKN
                     AVHYLHLLLTSGFNIKEFNLLYLAYPFFGIVITYLFVKYCVKDNISHGISKILRSISK
                     EQSIVKPHNMFTSIIASTFTVGFGGSVGLEAPIVLTGSALGSNLGRIMRLDYKTTTLL
                     VGCGAAGAIAGIFNAPIAAVLFSLEVLMLDLTMASLIPLLLSAATGTTLSYFLMGRGF
                     LFSFHVADPYKLNNLPFYILLGVFTGITSVYFTKANFSLESRFKAIGNSTLRTVTGGL
                     ITSLLIFLFPPLFGEGYTSLNALLEGHGNSIVEGSLFYGIQGNTLLFAGFLLLLIIFK
                     VIAMTATNGGGGVGGIFAPSLFVGGVAGYLMALVINIFLPGRLPVENFTLAGMAGVMS
                     GVMHAPLTAIFLIAEITGGYQFFIPLIFTAIVSYLTIMYFEPHSIYTKRLAELGELIT
                     HHKDKATLSRMTIENLIETNFLKVSPEATLGELVKIVSQSQRNIFPVVDGDNNFMGVI
                     FINDIRHIIFNHDLYDNVYVSNLMFMPDTFVNLDATMEDLAQKFHDTAHYNIPVLDKG
                     KYVGFVSRANVFSAYRKLVKEFSEE"
     gene            complement(57925..59967)
                     /locus_tag="PKI35_01555"
     CDS             complement(57925..59967)
                     /locus_tag="PKI35_01555"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNX43091.1"
                     /translation="MPNRLIRETSPYLLQHAHNPVDWFPYGDEAFAEAKRRNVPVLIS
                     IGYSACHWCHMMERESFEDAGIAGIMNENFVCVKVDREERPDVDHVYMNAVQLIQGQG
                     GWPLNCFALPDGRPFWGGTYFGPGPWKEILIRIAEFFRIRLADAEARASEIVRGMAGS
                     SLFAPEQQHPGFTKTDFETILTDLQSRFDSLNGGFGHAPKFPMPSVAGMLLHFHALTG
                     NRDALQMALKTLHSMAAGGIYDQAGGGFARYSTDAAWKVPHFEKMLYDNALLVSLYCD
                     AFKISGEELFRKVADETLAFVSRELTSPEGVFCSSLDADSQGEEGLFYTWTYPEFEEA
                     LGPYSSLMAEYFGVGKEGLWEHERNILLRPHNDALFAQRHFLSQDELSALVARVKRDL
                     HKYRNRRPRPATDDKVLTAWNAMMIKAYADAGMAFGNREYIETACQAAAFILNNMKNP
                     EGGLFRSWKNSRARIEGFLDDHAFMAGALLRLYQATSDEYWLGECSGLIAFILEHFQD
                     PASGLFFFSGEKSRLFAGTIETNDGAIPSSNSILAGLLFTLGTFMDYSSWCDTADRMM
                     ARTIAGAKEYPLGYANWCTLAMERSQPACVLAACGEKAGEYMAEFGKYFLPGVFIAGS
                     SKPSELPYFRERFNKNKTLIYICYGQACLAPVATVEEAIAILRERHSSENSFTSLR"
BASE COUNT        15739 a        14657 c        13995 g        15602 t
ORIGIN      
        1 atcaacccgt tggcgaccga aaaggtggtg tggtttggca tacacaggga agcggaaaat
       61 cgctttcaat ggttttcttc acgggcaaga tcgtattggc attagataat ccaaccattg
      121 tcgttattac cgacagaaac gatttggacg accaactgtt tgacaccttc gcagcgtcaa
      181 aacagctttt gcgtcaggaa cctgtgcagg ccgatgaccg aaatcaactg aaagaccttc
      241 taaaagtggg ttcaggagga gttgttttta ccaccattca aaaattccag cccgaagaag
      301 gtaatgttta cgagttgctt tctgaccgaa gaaatattgt tgttattgcc gatgaagctc
      361 atcgaacgca atacggtttt aaagccaagc aaatcgatgt caaagatgaa agcggcgatg
      421 ttgttgggaa aaaagtagtt tatggtttcg ccaagtatat gcgcgacgct ttacccaacg
      481 caacctattt aggcttcaca ggaactccga tagaaagtac cgatgtaaac actcctgctg
      541 ttttcgggaa ttatgtggat atatacgaca ttgcccaagc ggttgaagat ggggcaactg
      601 ttcgcattta ttatgaaagt cgtttggcca aggttaatct gagcgaagaa gggaaacaat
      661 tggttgacga gctggatgac gagcttgatc aggaggactt aaccacgacc caaaaggcaa
      721 aagccaaatg gacacaattg gaagcccttg tcggaagtga aaatcggatc aaacaaattg
      781 ctaaagacat tatcgaacac tttgaacaac gacaagccgt atttgatggc aaaggaatga
      841 tcgtaaccat gagccgccgt attgccgcca atttgtacga tgaaattacc aagctgaaac
      901 ctgaatggca ttcgcctgaa ttaaataagg gagctattaa agtagtgatg acttcagctt
      961 cgtctgacgg accaatgatt tcgaaacacc acaccaccaa ggaacaacgt agaagtctgt
     1021 ctgagcggat gaaaaatccc gatgatgaac tgaaactcgt tattgttcgg gatatgtggc
     1081 ttactggatt tgatgctcca agcatgcaca cgctgtacat cgataaaccc atgaaaggcc
     1141 acaccctgat gcaggctatt gcaagggtaa accgtgtgta taaagataaa cctggtggtt
     1201 tggttgtcga ttatctgggt atagcttccg acctgaaaaa ggccctttcg ttttattctg
     1261 aatcaggagg taaaggcgat cccaccattt tacaggaaca ggctgtacaa ttgatgcttg
     1321 aaaaactgga agtggtttca caaatgttta atgaattccc ctcctccgga agggtggtcg
     1381 aagaccgggg tggttttgcc tacgaagatt attttaatgc tggcacgtca caaaagctat
     1441 cgatgatttt agcagccgag gaacacatct taggtttgga agatggcaag aagagataca
     1501 tcaatgaggt tagtgctttg tcaaaggctt ttgccatagc cattccgcac gatcaggcga
     1561 tggatgccaa ggatgaaatt tcattctttc aggcggtaaa agcccgtttg gcaaaatttg
     1621 atggaacagg aactggccga accgatgaag aaattgaaac caccattcgg caggtgattg
     1681 ataaagcgtt ggtttcggaa aaagtaatcg atgtatttga tgccgcagga ataaagaaac
     1741 ccgacatttc aattctttca gaagatttct tattggaact gaaggggatg gaacacaaga
     1801 atgttgccct tgaagtcttg aaaaagttac tgaacgatga aatcaagtcg agggcgaaaa
     1861 agaacctggt aaaaagtaaa accttaatgg aaatgctgga aaatgccatt aagaaatatc
     1921 acaataaaat actgacagcc gccgaagtca ttgaagaact gattcatttg agcaaggaca
     1981 ttgttgaaat ggacaacgag gccaaagcaa tgggattaac tgaatttgag tatgcttttt
     2041 atacagcagt tgctgacaat gacagtgcaa gggagctgat gcaacaagac aaactgcgtg
     2101 aattagcggt tgttttaaca gaaactatcc gacaaaacgc atcgattgac tggacaatca
     2161 aagaaagcgt aaaagcgaaa ctgaaagtgg ccgttaaaag attgcttcgt aaatacggtt
     2221 atccgcccga catgcagctg ttagccaccg aaaccgtttt aaaacaagca gaaatgattg
     2281 caaatgaaat aacgatgagt gagaaccact gaccttatcc aagcatccca atttgcatct
     2341 gaactaaaac cggacaacct aaagcgttac gtaatgtttt gttataaagc atggaaggct
     2401 ggcgcgccgt cagaataatg ttatagacat caatgaaaaa taaatttgct gaatgtacga
     2461 gttaacttta cccattgctt cctctctccg tcatatcatc cctttcccgt tccctgatct
     2521 accgcggcag gtggcttaat ctgctgttaa acgaaggcag ctacctgatg agtaccctgt
     2581 taaaaaggcc ggtaatggcc ggattgccct ggtcggcatc catcgaacct acaacatcct
     2641 gcaaccttcg ctgcccggaa tgtccgtcgg gattgcgaaa attttcccgc cccaccggaa
     2701 acctgtcgat ggaggacttc agaaaaatga tcgacgacct cagcccgcac ctggcttatc
     2761 ttaccctcta tttccaggga gaaccttttc tgaataaaca ttttgccgat atggtcaggt
     2821 atgcaaaaaa acagaggatt tatgttgcca cctccaccaa cggccatttt ctggatgaag
     2881 aaacagcgaa atcagttata cgatgcggcc tcgacaggct gatcatttcc atcgacggca
     2941 caaaccagga gacctatgag aagtaccgca ggggcgggaa ccttgaaact gtaaaacaag
     3001 gcatccggaa tatcaccgat gagaaaaaaa ggctccgggt aaatcatccg tttttggagt
     3061 tacagttcct ggttacccgt ttcaacgagg gtcaggcaga ggctattaaa tcgtttgcca
     3121 aagaagtaaa agccgataag ttaactttta aaagtgccca ggtttattcc ttcgaagagg
     3181 gcaatccgat gattcccttg gcagaaaagt attcaagata ccggcagatg cccggtggcc
     3241 aatggcagat caaaaacaag ctgccaaacc gttgtcaccg tttatggcat gcgccggtta
     3301 ttacctggga tgctaaggta gttccctgct gtttcgacaa ggatgccgtg tacatcatgg
     3361 ggaatttaca ggaaaaatcg ttcagggaga tatggaaaag cccggagtac cgggctttca
     3421 ggaaaaaagt attttcggaa agaaaggaga taaatatttg cacgaattgt accgaaggat
     3481 tgcgatgacc tttataccgt catgatatcc ttttcctttg cttcgagcac tttatccact
     3541 ttactgatat actggtcggt gatgttctgg atttcttttt ccagcacttt gatttcgtct
     3601 tcgggtacgc cttccttttc aagctttttg gcatcttcaa gggctgaacg gcgcatattc
     3661 ctgatcgtaa ctttagcgtt ttcggcttcg tttttggctt ttttcaccag gtcgcggcgg
     3721 cgttcctcgg taagaggggg cacggcaata cgcaatacct ccccgttatt ctggggattg
     3781 aatcccaggt tagcttccat aatggctttg gccagcggag ccagcatact tttttcccag
     3841 ggttggacca caatcaggcg ggcatcaggg gtgttgatgt tcgaaacctg ttcgacaggc
     3901 gtcatggttc cgtaatagtc aacttttacg ccctcaagca tttgcgggct tgctttccct
     3961 gtacggtatt tatgaaattc cttttcgagg tgctgcaggg cgttggccat gctttcgttc
     4021 atctcctcaa ggataaattt cgcttcttca gtcatatttc aggtgattaa gttcagtaca
     4081 aaaataaatt catttttcga gcgggcaaaa tttgtgccga tcggatcaaa tcaggtgaat
     4141 tgtatccgtt aatttgaaac cagcgttccg acattttctc ccctgatgat tttcagcagg
     4201 ttgccggctt tgttcacatc gaagacgatg accggcatat tgttttctcg gcaaagggtg
     4261 aaagccgtca aatccatgat tgaaagtcct ttgtcgtatg cttctgcgaa tttcagcgtt
     4321 tcgaaacggc tggctttcgg gtctttttca ggatcggcag tgtaaacgcc gtcaacacgg
     4381 gtccctttca gcagcacatc ggcatgaatt tccacggcac gcagggcggc agccgaatcg
     4441 gtagtaaaaa acgggttgcc tgtgccgccg gctatgatac atacttcacc ggactgcatg
     4501 ctttggatgg cgcgcggcga actgatacgt tcggctaccg ggtcaacggc aatgcccgag
     4561 agcaccctgg ctttaatccc gatattttgt aatgatgcct gcaacgccag gctgttgatt
     4621 accgtggcaa gcatgcccat gtaatcgccc tggatacggt cgatgccggc gttgttgccc
     4681 ttcagcccgc ggaaaatgtt tccgcctccc agcacaacgg acacctgggc gcccaggtcc
     4741 acaaccgact ttatttcacc agcatataaa tcaagcatcg aagcatcgaa gccataccct
     4801 gaatttccgg ccagcgattc accgctcaac tttaataata tccttttata tttcattatc
     4861 ggcgtttttt tgataattat gatcaatcag taagtcatat caccatccgg gatgttgcta
     4921 cctgacaata agcagcgctt tagtccgcga cacagaaccg tttgccagcc ggtaaaaata
     4981 tgttcccggc ttatatccgg aagtctccag gggaataaaa tgcgtcccgg cagaatagtt
     5041 cccttcggcc aaagaagcaa ttttccttcc gtaaaggtcg aaaagtccga gactgatttt
     5101 cgattccctt tccagattaa acggaatggt tgtggtaact gctgccggat tgggaaagtt
     5161 ttgccccagt gaaaaagcgg aagcctgaag gaattcgggt accgtaacag acatatcata
     5221 atataccaag gttatacacc agttatccag tgttcccgta tggctgatga tactgtcctg
     5281 aaccctgagt acccagctcc cggtgatatt tgtgttatcg aagctgctca gcggctgttc
     5341 gggtttgaac ctgccgataa aaggagcatt acccatccgg atggaaaccg aagcttcatc
     5401 atcaaaaatg gttccggtat agttggcgtt gttacccccg ttcccgctgc tcaggtcaac
     5461 cacagaaccc agcggactga taagcgagat gttcagggtt ttatccatcg tatgggtaat
     5521 attcaccgtt acatcgatat cctgtactcc tcccaatata tcaaccggaa tggtatcgta
     5581 caccgagcca agatcgggaa tgggaaccgg gaccgtgttg ctgcataaag tgaccgcttt
     5641 taccggggct atataataag tgaacgggac ttcgggggct gcggttcccg gcggattgat
     5701 gccacgcccg ccggcgggca gcgtacctgt aattatccct gccgaatcct gaacggcaaa
     5761 ataataggaa acggcagtgc caggctgttg ccccgggatt tcaaaagtaa actggttatt
     5821 tgccgacgaa accggtaaaa ggtactggta ttcgttcccg ttcgtcttgt aatagagtcg
     5881 gggctgatac aggcctgtgg cgaccggcct tggcgacagt acgttaaaga cagcttcacg
     5941 gggattggta tcatcacccg aagccaccgg ttcgtggagt atctccagac ggcaattcca
     6001 gcccagggtt gccaatccgg ctaccgaggc ctgtaccatc cgggtaaaga aaggaatatt
     6061 cagaatggaa aactggtcct gctgcgtatg gtaatactcg ttgaaatcac cgggatactc
     6121 ttcaatccct aggatggccg ggtatccgtt atgccagaag gacgaatggt cgctggcttc
     6181 aatggaagtg tagttccagt tgatctcagg agtgtacata cggagaacat cctgataatc
     6241 gttggtgagg tcctgtgaca atgcattcgt gcctatagtc atcttatagt cattattcga
     6301 atcccaggct atcatgtcga aattgagaac gcccaggatc tgttcgccat tgaaagccgc
     6361 gctgtcggaa aaagccttgc tgccaaccag gccaatttct tcttcatccc aggcggcaaa
     6421 caccagggta taatcgcttg gccatgaaga aaggatccgg gcagcttcaa gaacagctac
     6481 aacgcccgaa gcattgtcat cggcgccggg cgcgaaagaa ttcgacggca tatcatccat
     6541 atgggcacag ataatatatt tcttatcggg atatttggca ccggtcttcc agccgataat
     6601 atttcgtccg gaggtactgt aatcctgata cctggcctgc aggccataac tctcgaattt
     6661 ttcttttata tactgggctg caatattctg gattgaatta tactggtaat tcctggatgt
     6721 gatggtttgc ggtgtccccc ctacaagcac gtttgtatca cccgatagtt cccttaccgt
     6781 caggctgacg gttcccggtg ttgccaggtt tatcagagaa tcaatcacag gactgtaggc
     6841 aatttgtgat ttcagggtcc agggaaccat aattagcaga aacaggctga taaaaaacgg
     6901 cagcagaggt tttctcatta ttttcaggga ttaccaggat taatattttc aggagggggc
     6961 atttaatgtg agcgttggat gcagcgttta attattcttc acggccatgg cagtttttgt
     7021 acttcttacc gcttccgcaa gggcacggat cattccttcc gactttcttt tccactttca
     7081 ccgggccggt aacggtattt tcgccggtac gctggttaat cacgtcggta cgctcttcac
     7141 gtaaccgggt gtcgctgcgg cggctcggca tctgcgcttc gcggacctgg tcaggctgct
     7201 ggacgggtat attggcccgc aggataaacg agataatatc cttatttacc ttttgcagca
     7261 tggttttaaa caggttaaaa gactcgaatt tgtaaatcag caacgggtct ttctgctcgt
     7321 aggaggccga ctggacgctc tggcgcaggt cgtccatttc gcggagctgc tctttccatg
     7381 catcgtcgat gattgccagg gtaatgcctt tttcaatggc gagaatcact tccttaccct
     7441 tgtcctcgta agctctttta aggctcgggg taacctgcag ggtcttttta ccatcggtaa
     7501 tggggatagc cacatacagg tagcgcgggt tgttttcata aatatcgcgg atcaccgggt
     7561 aggtatgctc ggcaatgcgg tttgacttgt cccggtacga cttgtaaaca gaatcgaata
     7621 actgttgcgt cagtttttct ttgttttcgg ccaggaattc cttttcagta aacgggcatt
     7681 cggaggagaa agtcgtaaga aggtccagct taaagttctc ataatccccg ctttcctggt
     7741 tgtctgttac cattgtctca gccaggtcaa agatcatgtt actgatatca acggcaaggc
     7801 gttcaccgta caaggcatga cgccttttgc tatagatgac ttcacgttgc gaattcatca
     7861 cgtcgtcata ttcgagaagc ctcttacgga tgccgaagtt gttctcttca actttttttt
     7921 gcgccctttc aatgcttttg gtgatcatgg aatgctgtat cacttcgcct tccttcagcc
     7981 ccaggcggtc catgatcccg gcaatacgtt cagagccgaa cattcgcatg agatcgtctt
     8041 cgagcgaaac aaagaactca gagctgccgg ggtcgccctg acggcctgaa cgtcctcgca
     8101 actgacggtc gacacggcgc gattcatgcc tttcggtacc gataatggcc aatccgcctg
     8161 cctctttgac acccgggccc agctttatat cggtaccacg gccggccatg ttggtggcga
     8221 tggttaccgt tcccgcctta ccggcttcgg caacaatctg ggcttccttt tggtgcaact
     8281 tggcattcag cacattgtgc gggatacggt cacgggtaag catacggctc agtaattcag
     8341 aagtttcgac tgaggtagta cccaccagaa caggtcgtcc tttgctgatc agatccttta
     8401 tttcaataat tacggcgtta aacttctcgc gtttggtttt gtaaacaaga tcttcacggt
     8461 cgtcgcgcag aacagggcgg tttgtgggga tcgccaccac atcgagttta taaatatccc
     8521 agaattcacc ggcttcggtt tcggcagtac ccgtcatacc ggccagcttt ttgtacatac
     8581 ggaaatagtt ctgcagggtg attgtggcat aagtctgcgt tgcagcttcc accttcacgt
     8641 tttcctttgc ttcgatagcc tggtgcagcc cgtcgctgta acgacgtcct tccatgatac
     8701 ggccggtttg ctcatcaacg atcttcactt tattgtcgat cagcacgtat tccacatcct
     8761 tttcaaacaa ggcatacgct ttcagcaact ggtttaccgt atggatacgg tcggatttta
     8821 cagaataatc ggccaggatc tctgcctttt tctccatacg ttccttttca gggagagggg
     8881 atttttccag gtccgccaat tcggccccta catcgggaag gatgtaaaat ttcgggtcct
     8941 ggtacgactt ggtgatcagg tcaataccct tttcggtgag ttcaatctgg ttgttctttt
     9001 catcaatcgt gaaatacagc tcatcatcaa tgataaacat attcttcgat tgttcctgca
     9061 tatagaagtt ttcggtcttg agcagaattg ctttgatacc gggttcgctg aggaacttga
     9121 tgagcggcgt gtatttggga aggccgcggt gggcgcgcaa cagttgttcg ccgccgcgct
     9181 tttcgttttc aggattaatt ttcccgtcag gcccttgttc aagtaacttt ttcgattcag
     9241 ccagcagttt gttgaccagt gaacgctgtt cattataaag cctggaaatt tcaggtttca
     9301 gtgtttcaaa ctcctggttc tcgccgcggg gcgtaggccc tgaaatgatc agaggggtac
     9361 gggcatcatc aataagcacg gagtcaacct cgtcgacgat ggcatagtta tgaggccttt
     9421 gcaccagttc atcggggttc cgggccatgt tatcacgcag gtaatcgaag ccgaattcat
     9481 tgttggtgcc gtaagtgatg tctgccaggt aggctttgcg gcggtcttcg gaattgggtt
     9541 cgtgccggtc gatacaatct accgtaagtc cgtgaaattc gaataatacg cccatccatt
     9601 ccgagtcacg tttggcaagg taatcgttta cggtaaccac atggacgcct ttaccaggga
     9661 gcgcgttaag gtacacggga agggtagcca cgagggtttt tccttcaccg gtggccattt
     9721 ctgaaatctt tccctggtgc aggacaatgc cgccgataag ttgaacgtca taatgcacca
     9781 tatcccaggt taccatattg ccgccggcca tccaccggtt atcccagtat gccttttcgc
     9841 cggcgatatt tacgttgtcg cggcgggctg ccaggtcgcg gtcgaattgc tgtgccgtta
     9901 cctctaccgt ttcgttttcc ataaaacggc gcgccgtatc cttcatgacg gcaaaagcgg
     9961 ccggcagaat ttccatcagc gtatcctgcg tctttcggta agattccttt tcgaggttat
    10021 ctatcttttt atacaattcc tctttctcct cgatatccat ttcgtattcc gtatcgaggc
    10081 gcaaccggag aacagcaatt tcagattcct cctgttcaat ggcccgggct atctgttccc
    10141 tgaattctac cgttttagca cggagttcgt cgttactcag cctgacaatc tgctggtatt
    10201 cctgttttgc cttctcaaca aggggcataa tcgctttgat gtcgcgttcg ctcttggtgc
    10261 cgccggcaaa catcttgaag atataattga gcataatttt atggaatatg acgatgtacg
    10321 gatcaaatac catacaacca tgaagcaaaa gtaactgatt ttatccgaat tattttgaca
    10381 ggaacggcca aaacaagcag caggcataga acatggggac aaacagccac tttacaggat
    10441 agtatcgtga tgacggatat aaatgacaga atggcggata caggaagccg taccgttaaa
    10501 aaaaggattt caaaccgcaa attttaccag gttgaccggc atgagccagg aagcaacggc
    10561 cacagaaacc ccggaacaga aaacccggat caacaacacg gatttcaata gcctgacatg
    10621 gataattccg gagtatgcgt ccggcttcag gggaaagcgg gcagcgcatg caataaagga
    10681 aaacgaagtt accggtatta taggaaagaa ttttgttatg caccgggtct tctgaaccgc
    10741 cggcaaccat cattatgccg cagcaaacta gcgggctgaa atgatcgaca aaccgcaaat
    10801 aaacacccgg ggcggccagt cagccgcccc gttttttctt taatactcgt cttcatggaa
    10861 gaaaaaatct tccttcgagg gataatcggg ccacaaatct tcgatgcttt cgtaaatctc
    10921 gccttcgtct tccagctcct gcaggttttc aactacctcc atcggcgccc cggagcgaac
    10981 acagtagtcg agaagttcat ccttggtagc cggccaggga gcatcttcaa gtttcgaagc
    11041 taattcaagt gtccaataca ttatatagtg tggttgttag gttgtaattt tttgcaaaaa
    11101 taacccttaa aaataaaaaa gcaagtgttc agatatattt aatttatcat tactcattaa
    11161 gctgatttac tgtctgttat tacatttttc gtgttcttgt tgtgtatgca gccctgatcc
    11221 ttttaccagg gaggatgcaa aaagccgtca gaaaagtgtc agtttgctgt ccgaacatga
    11281 attttacaac ccggttttat cgtttaccgg aaattcaggc atcattggtt ttagctaatt
    11341 tattatgctt tagcctgaac gactggaact taaaatgatg aaacccaacc agcttcgtct
    11401 ttgtaagcag gcctgcggca tgaaccaggc agaaaggatt tcatcgttca gctcttcctg
    11461 atcccgcatt ctgtaacact gaaccggatt gcatcaggca tttttcagga ctctaacaaa
    11521 ttattttcaa attcccctgt aacaaatcat acctctctgt cgtctagcaa tcgaaataat
    11581 ctaacgatga actaaaccta atacacccta ccatgaaaac ttttaaaaac gtcctgttca
    11641 tagcgctttt atcgtttctt gcaacaccgt tgctgatggt tgcccagaat ggcaacggca
    11701 acgtggcaac ccaggaacgc cggcttgcat ccttcagcaa cattgaagtg ggcgcagcgg
    11761 tcaatttgtt tctatcccag ggttctgccc agtcggtaaa agtggaaatt gatgaaaacc
    11821 tcctcgaccg cttcgaaacg gaagttgatg gcaatacctt atccattacc gctaacgggg
    11881 tgaacaatgc tactaaaatg aatgcgtatg tcaccgtcac ctctctgaaa agtatagaag
    11941 ccaataatgc ggcaactgtc acaggccaga ccgcacttgc ttcagacaat ttttcatatg
    12001 atggttcagg cgcaaccaaa gccaatttcg agatgaattc cggcgaaatg atgatcgaac
    12061 tgagcggggc cgcgaatgcc atcatcaaaa caaatgtcaa cgccctgaaa acagatatca
    12121 gcggcgccag cgatcttacc cttaccggta cggcaacaca gcacgacctg gaagtaagcg
    12181 gcgccgccca cctgaaagcc atggaactgg ttaccgataa taccagcgca gaagttacag
    12241 gcgcaggaaa tgcgaatgtt atggctaaaa tacagttaaa aaccgacgtg tcaggcgcag
    12301 gcaacttatc gtactatgaa aacggcgaaa tgcgtcatat cagcgccccg aacgaaatga
    12361 tgaaatcctt ttcgggcatg gaaaacatca aaagcgtgat cattgagaac gacaacgatg
    12421 aggaaggcaa tacacgtttt gagatcaggg atggcgaaga tactgtaaac gtgacgcttg
    12481 gctccgacaa aatgattatt gtcagcgacg ataccgtaaa agtaaaactc ggccgtaacg
    12541 aggtcatcgt gaacgacgaa ggcaatgtga aggttaagca cgccaagaaa aacaagttta
    12601 acggtcactg gaccggcctc gagattggga ttaacggtta tctgacgccc gatatggatt
    12661 tcaattatcc tactgattac agcggccttg agcagaatta tgtgaaatcg accaatgtaa
    12721 acctgaatat ttacgagcag aatatcaatc tgataaagaa taaacttggt ttggtgaccg
    12781 ggttgggatt tacctggaac aactaccggt ttgatgcgcc cgaaacaagg ctggccaaac
    12841 gcgacgggga attatcggta tacagcgacc agaatccgga tttcagctat agcaagagca
    12901 aactggtggt tgattatctc acggttcccc tcatgctcga ataccagacc aattcccatt
    12961 cgaaagtcaa cagctttcat ctaggggcag gggtagtcgg cggcctgcgc atcggctccc
    13021 ataccaaata tgtttatgat gacggtagcc ggcagaaaaa gaaaaaccat gaagatttct
    13081 acctgaatcc tttcaaagta gacggcatgg ttaaaatcgg ctggggcgtc atcaatcttt
    13141 acggaaccta ttcattaacc cctctgttcc gttcaggaaa agagcccgaa ctgtacccct
    13201 ttagtgttgg catctgcctc acagaccttt cagaccttta agccgggaat ttcaggccat
    13261 actcattccc tgcctataga aaaaacatac aaaatctccc tgtatcaatt atcccatatt
    13321 ttcttattac tggttagtgt tttgctggct gtcgcagaaa cgatctgcgg cagctttttt
    13381 gtttccgtcg attatattat ttttgcactt tcatagttat aaacaactat tttatagcta
    13441 aatagtccga agctgcaatc ataaaaacac aattcccatg tcaggacata ataaatggtc
    13501 aaccattaaa cgcaaaaaag gggccctgga tgccaagcgc tccaagattt tttcaaagat
    13561 tatcaaggaa attactgttg ccgtaaagga aagcggcgcc gatcccaaca gcaatccccg
    13621 tcttcgtctg gctattgcca atgccaaagg ggccagtatg cccaaggata atattcagcg
    13681 ggctatcagc aaaggggcag ataaagatgc ggcttcgttc gtagagacta ctttcgaagg
    13741 atatgccaac ggaggcatag ccatctttct ggaagccact accgataata cccagcgtac
    13801 tatttcgaat gtgcgttcgt atttcaacaa atttggcggt aacttaggca ccaacggttc
    13861 gctgagcttt atcttcgaca ggaaaggaat ttttgccatt ccgcagggca atcttgaccg
    13921 tgacgaattt gaaatggaga tgattgatgc cggagccgaa gatattcagc ttgaagacgg
    13981 cacattcact attactactg ccatggaaga tttcggcccc atgatgaaga aactggaaga
    14041 aatgcatgtg gaaccggaaa gcgccgaatt acagagaatt cccaaagaca ccaccaccct
    14101 tgacctggaa tcggctaaaa aagtaatgcg gctggttgat atatttgaag aagacgacga
    14161 catacaggcc gtataccaca atatgaccat caccgatgag ttgatggaag cattggatta
    14221 aagaagcgtt tcgccagtgc gccatgattg ccacgggaag ccggtaatcc gtccgggcct
    14281 gtgtttcttg atgcccgcac tataaaaaat aatagaggct gccccgtgaa acggggcagc
    14341 ctcttcgttg atttaaccgg aaattattac ttcgtcacaa tttcgtaagt atcctgggct
    14401 atcacgagtt cttcattggt aggcaccacc atagcaacta cttttgaatc atcggcagta
    14461 atgacggctt cctttccacg gagacctgca ttgcgttccc tgctgaattc gagacccatg
    14521 aattctaatc cccggaggca gtcttcacgg acttcacatc cgttttcacc aacgccgccg
    14581 gtgaaaatga tcacatcaac cccacccatg gccgcagcat aggaaccgat atatttcctg
    14641 atgcggtaac tgtaaatatc cagggctgtt tttgcgcgtt tgtttccttc ttcagccgct
    14701 ttttccacat cgcgcatatc gctcgaaacg cctgaaaggc caagcatacc gctgtgttta
    14761 tttaacaatg tgttggtaaa tgcaatgctc gtctcttcct tatcggcgat gtacagcacg
    14821 gctcctgcgt cgatgtcgcc gcaacgggta cccataacca acccttcgac cggggtcatg
    14881 cccatgctgg tatcaaccga ttttcctccg tcgatggctg ctatggaagc gccgttcccg
    14941 aggtggcaac tgacaatttt caggtctttg atgtctttac ccagtatttc ggctgcccgt
    15001 ttcgagacaa aacgatggct tgatccgtgg aaaccataac ggcggacctt atatttttca
    15061 tacagcgagt aagggatccc gtaaagaaaa gcatactcgg gcatggtttg atggaaggcg
    15121 gtatcgaaaa cgcctacctg gggcacttcg ggcaacaggt tggtgatggc acgaattcct
    15181 tcgaggttgg gcgggttgtg caacggcgcc agatcgatgc actcttcgag cgcacggata
    15241 acctcggggg tgatggcaac agagccattg aatttttcgc cggcgtgaac cacacggtgc
    15301 cctacagcgc ctatttccct aaggtcgcca acgctgccgt attttttatc ggtaagtatg
    15361 gccagcacat gctctatacc tgcctggtga tcgagtattt ccccttcaag tttcaacaca
    15421 gtcccatcat tacgggtgtg ttttatggat gctcccctaa gcccgatctt atcaaccagc
    15481 ccctttgcgg caacttcggc cgagggcata tcgaaaagtt ggtatttgat ggatgaactt
    15541 ccgcaattca atacgataac cttcatcgtc tgatgtattt tatgttttat aattcaatta
    15601 tttaagttca aggcaattta ccgaaaagcc aactactttt ccattggcat cgtatttata
    15661 aaacccctgt ccggttgatt tgccaaaatg attggcgcga accagttttt taataaccgg
    15721 cgatgtctta taagcttttt cgccaaattc attgtaaatt ccttccatcc atttcaccac
    15781 cttatccaag ccgatacggt cggctaactc gaacggcccg tatgtgtatc cgaggttcat
    15841 tttcagggtt tcatcaatat cggggatgga ggctattcct tccatcagca tccggcaggc
    15901 ttcgttgatc atgatcacga tcatacgcgt tgtaagatta ccgggcgatt cctgaagcag
    15961 gatcggcttc ttttcgagca tatgcgtgaa acggcagatg gtgtcgtaag ccgcttcgga
    16021 agtttttgcc ccgcgaacca cttccagcac ttttacctta tcggcaggag ccatgaagtg
    16081 aaggcctacg gcacgttcag gatattttaa tccggcagca agatcggata tcatcaggac
    16141 tgccgtgttg gaagagatga cggtatcgga gccgacaaac tgttcgattt tgttaaaaat
    16201 attcctgcgc agttctacgc ccgccccagg cacctttgag ctaacgctct caataacgat
    16261 atcgcaaccg ctgagatccg cataattgca cgaaccgctg atacgcgaca ggataagccg
    16321 tttttcactg ttcgtcatgc cccactggcg gatttcatcg tcaagcatgg tggtaatgcc
    16381 agccatggca tcggccacat gctgatcatc aacgtccagg cataccacat caaaaccatg
    16441 ctggctgatg gttttcgcta tctgctgccc catggatccg cacccgatta tgcccacttt
    16501 ttgtattgta ccttttggct gctcctgacg gctcagggta aaatcttcaa gttttgcaat
    16561 tatttctgcc atttcttcag ttgttttaag gttcccggtt atgatcaaaa agccccatga
    16621 accggatttt atttgttgaa tatcaatatt ttagctttac tgccgaaatt tgacgcaaag
    16681 gtagaacata ttcaacgatg attttcggga tttgtcattt tttcaaagga tttcattgac
    16741 atatcggccg aaaatgaatt tcacctcaat ggtttctttg tatttttgca gccagtataa
    16801 aggttatgct tacggggatt ccacgatata aaaccaaccc gctgatttcc gacacaaccg
    16861 gattcttcaa tgcgtacatt ccttatactg ccgaaaagca tcaaaccacc aacgattcac
    16921 tgctccgcta ttattactac ccgatgccgg cagttacccc gggcaggaat gatacaataa
    16981 gtattcatga aacgtcctcg gtttttgctc cacatcagtt acagccaatc cataaaaatc
    17041 ccctggcaaa caagccgctc tcagccgact ggatcacagg cgtttttatg ctctgcctta
    17101 tcatcctcgc agtcgtgaag gcatccttgc ccagacggct tcagcaaata ttcaggtcgg
    17161 ctgcccagct ttattatgta aaccagcttg aacgtgaagg gaatctgctc aaggaaagca
    17221 taagcttcgc attagggttt ctctatttaa cctctgtctc ctttttattg tataagctgt
    17281 cgatcctttt ttcgcccgga atcctggccg ggattaaaag ttttttcctc ttctcggcga
    17341 ttttcctggg ctgcctttta tactatatgc tgaaaaccgt tttggtttcg tcggtaggaa
    17401 caatttttaa aaccccgaaa gcaacccatg attacctgat taataattta atattcagca
    17461 tagtaaccgg catattactc tttccggcaa ctattctggc tttatatata aacaacccca
    17521 tatatgtatg gatagcctgt tcaattttct ctattctatt ggtatacaaa tttataagaa
    17581 cttttatgat cggattggca aatacgaaat actcgatgtt ctatttattt ttgtaccttt
    17641 gcacgatcga aattttgcca tttttgttgc tttttaaaac cgttcaacag ttttaaaagc
    17701 tattgattaa taggtagtta ttcattatca acactaaata cgactgcgca ttgaaagtaa
    17761 aaaacatact tatttcgcaa cccgttccgg ttgaccttga aaaatcgcct tatgccgaaa
    17821 tcatcaggaa atacaacgta aatgttgaat tccacaagtt tttcagggtt gaaggcgtaa
    17881 acgcccgtga gttccgtgat gaaaaagttt acatcaacga gtacacggca attattttca
    17941 acagcaagca tgctgttgat cacttcttca ggattgccaa agaagttcgc atcgaagtgc
    18001 ctgaaacgat gaagtatttc tgcatgtcgg aatcagtggc ttattacctg cagaaatatg
    18061 tgcagttccg taaacggaaa attttccatg ccgaccagga tccttcacag cttatcgatc
    18121 tgcttaaaaa acataagacc gagaagtatc tgattccctg ttcaaatact tacaatcccg
    18181 agcttacagt actgcttgat gccactaaga ttgaatataa aacggctgtc ctttaccgta
    18241 ccttgcccga tgatatgcgc gacaccgtag acctggctaa gtatgacctg ctcgttttat
    18301 tcagccctca gggagtaaaa tcactttttt acaactggcc tgaattcaag cagggcgaaa
    18361 aactgatagg ggcattcggc gtcaatactg cacaagccgc aaccgaggcc gggctggtcg
    18421 tcaacatccc cgctcctacg gccacggctc cttccatggc tatggctatc gaccagttca
    18481 tagtcaaaaa ttgtaaaaag tagccttcca tttatttaaa gggtccagcc tgaaagcctc
    18541 cggctctttg gtcagggtat catgggcggg aattctgtga ccaggacata atccggtttc
    18601 tgcaatgtaa tgttaactac ctttgtccgg cgatgccaag catgaaacct gtaacccgac
    18661 acacttttcg aaaaacggaa aaactggtca gccggaagct gatgaatcaa ttgttttcgg
    18721 aaggcaaatc cttttttatt ccgcctttta aggttttatg gctggatacg accgttgacg
    18781 ccaaatcgcc ggcacagttg ctcatcagtg ttccgaggcg aaatttcagg cattccgttg
    18841 accggaacag aattaaacga ctggtacggg aagcatacag aatccataag catcagttgt
    18901 atgcagttct ttcatctcag ggcatccagt gtgccatcgc ccttgtgatg acaggccgga
    18961 atatgcctga ttataaagaa gccgaagaaa taataatgct aattttacag cgtttatcag
    19021 acgaacatga gaaagttgcc ggctaaaata ttaacaggcg tgataagatt ttaccaatac
    19081 gccatttctc cttacctgcc gccatcctgt cgttatacgc ctacctgttc ggcatatggc
    19141 attgaggcaa ttcagaaata cggcgccgtg aagggggggt ggctcaccct gaaacgcctt
    19201 gcatcctgcc atccctgggg tggcagcggt tacgatcctg tcccatgata aaacttcacc
    19261 atgaaaacat cctcccgtcc tatgaatttt ataagaaacc atttcgcggt tttcgcctta
    19321 ctggtaatcc tcggattatc ttccgcaggc gtgaaagcac aacaatcgca ggaattcgaa
    19381 atctcaaaga acctcgacat atttgtcacc ctttataaag aactgaacaa caattacgtg
    19441 gatgacatca acgcctctga gctcatgcag acgggaattg atgccatgct cgaagatctg
    19501 gacccctata cggtctatat cccggaatcc gaaattgaag actaccgttt catgaccacc
    19561 ggcgaatatg gcggaatagg atcgctgata catcagcaaa acggaaaaat catcatttcg
    19621 gaaccttatg aaaattcccc tgcccaaaaa tcaggtctca aggccggcga ccggattata
    19681 aaagtaaatg acaagaccgc agaaggaaaa tcggttgaag acgtcagcag tatattgaaa
    19741 ggtcagcccg gaactaccct gaaactggaa attgaaaggc cgggaatgaa caaaccgctc
    19801 aattttaata ttacccgtga aaaaattgtc ctcgacgccg ttccttactt tggaatgctc
    19861 aacgataaaa tcggttatat caagctctca agttttacgc aaacagcgtc ggcagaggta
    19921 aaaaaggcgc tgctggaact aaaacagaac cccggcatga aaggccttat cctcgacctg
    19981 cgcgacaacg gaggcgggtt gcttaccgaa gccgttaaca ttgtcaatct tttcgtcaat
    20041 aaaggcgaac tggtggtaag caccaagggc aaacagatgg acaagaacaa aacataccgc
    20101 accatggtcg acccggtaga tcccaacatc cccctggcag tattggtaaa cggggccagc
    20161 gcgtcggctt cggaaatcgt ttgcggcgct ttgcaggacc tcgaccgcgc cgtggtgatc
    20221 ggacgcaaca ccttcgggaa agggcttgtt caaaacatta ttccgcttac ctataacacc
    20281 caggttaaag taacggtagc aaagtattac atacccagcg ggcgctgcat ccaggccatt
    20341 gactatgccc acaaggatgg taacggaaaa gccggccaca tccccgattc gcttattacg
    20401 gcatacaaaa cgcaacacgg gcgtaccgtt tacgacggca agggcattgc ccccgatgtt
    20461 gaactcgata ccacccgctt cagttcggtg acctttaacc tgtatgccaa atacctcatc
    20521 ttcgacttcg ccaccaaata tgctgctgaa catctgcaaa ttgactctcc ggaccagttt
    20581 aagatcactg acgaaatttt caatgatttc aagaaatttg ttgccgaaaa aggatttgaa
    20641 ttttcaaccc cggctgataa agatttaact gatttaaagg cttctgctga ggaggaaggt
    20701 tgttgggaag atattaaaaa tgattatgaa gcgcttaaaa ccaggatcga aaaccacaaa
    20761 tcacaggata ttgacaacaa acgggatgaa atttcgcaac tgctgcaact ggagatcgtg
    20821 agccgctatt attaccagaa aggacgtatc gagtcctcgt tatccattga taaagacttg
    20881 caggaatcta ttaaaatcat caacaacagc cctgtataca cggctatcct tgacgggacc
    20941 tacgtaaaac cggaaaaacc tgcgtcaggg aaaaagatct gaccgcgata agcctgctat
    21001 catgaagata ctccgtcagc agattaactg gaaccgggat tccgcctatc atcttgtact
    21061 tttcctcctg atggcgtcga tcccgctgtc gaaatacatg acaagcgttt tccagtttgc
    21121 cctgaccgtc ctgtggctgt ggcacggcgt taacacttcc tatctcctga atttcaacaa
    21181 tgccaactgc ctgtcgcccg ttatctgggg ccggtttatc ggccaatccc taaaggaaat
    21241 atcaattgct tttattaaca aatggaagga tttcttcaga aacaggcctg cgatgcttct
    21301 ttcatcgctg tatgttttat tgcttatcgg cgtattatgg actaccgatt tacattatgc
    21361 gctgaaagac ctccggacaa aagtgccgtt gttcattctt ccgcttttct tagccacagg
    21421 tcctaaagta aatacgcgaa cgcttaacct gatgctggcc ggctattgcc tggccctttt
    21481 cggagggact atttaccagt taatgttgtt atctaacctg ccggttgctg atacgcgcgg
    21541 catttcagcc catgtatcgc atatcaggtt cagcctcaac ctggttttag gaatttattc
    21601 cctgctttat cttgcctgga agaaattttt ccgccaaacc gcggtcaaca tcggtttggt
    21661 gacactcgcc ctctggttcc tggccttcat gatatatatg cagtatgata ccggtgtcgt
    21721 cctcactgcc atcgtagcaa ttatcctgtt gtttttgctg gctttcaggc aatcgggaat
    21781 aaaatcgcgt attgccctgc tactggcatc ctttctgatc cttgcagttc ctctgattta
    21841 tatctcaacc gtaattcatg aattcaggtc ggttaaacct gtttatttcc tgaaactgga
    21901 tcaatgtacc aggaacggga atctttacac ccacgacaca accaactttg tggttgaaaa
    21961 cggcgtttat cccggccttt atatttgcga taaagagctg agtgaagcat ggaaaaaacg
    22021 cagttctata ccctatgacg gttatgacca caaaaaacag ttgatcagga gcaccatcat
    22081 ccgttatatg gcatcaaaaa acctgcggaa ggatgccgaa ggggtaactt cactgacgaa
    22141 tcaggatgtc agggccattg aatcaggcat tgccaatgta aggagcatca atggcttcaa
    22201 cattaagacg cagatttata attatattat agcgtaccgc aactaccgtt accggggcga
    22261 tccgaactcc ggatcgctgg tacagcgact ggaatactgg cgcacatcat ggctgatatt
    22321 ccgtgatcat cccgttgcag gagtgggcac tggcgacctg ccggcagcct ttgaaaagca
    22381 atacgaagcc atgcattccc cgcttaaacc cgaattcagg aggcggtcgc ataaccagta
    22441 cctctctacc ctggtagcgt ttggagtttt ggggtttgcc tggtatatgc tggtgttgat
    22501 atatacccct gtcaggctga aaggttttaa gcggtactat ttcctggttt attacatcat
    22561 catcatgatt tcgatgatca ccgaagatac actggaaagc caggaaggag ctaccttttt
    22621 cgcctttttc atttccttat tcctgcttgc ctggaataca gaaggccttc aggatttctc
    22681 cccggttcga cggaagaaat aataactctc tggttcagtt gttcccattt ctttaaccat
    22741 aggacactca ggggcactta ggattattaa ccacggagcc atggagaaca tggagcattg
    22801 ctttattctc agtgacctgg tgcccttgat gccttggtgg ttcccttttc tttttaccac
    22861 gaagccatgg aacatggagc attgctttat tctcagtgaa cctccatgcc ctcgatgcct
    22921 ccgcggttcc cttgtttctt ttattcaaga atacgcgtga actcagagca tttaattgtc
    22981 cttagtgaga ctcggtgccc taagtgcctt ggtggttccc ttttctttct accacggagc
    23041 catgaaggtt tcactaagaa agttaggccc ttggttcgca ttatgaatta tctttaacac
    23101 caaaaaccgt gttatatgaa tgaacgactg atttctattt catataccga attcaatcat
    23161 atcggtgaaa tggcaggaac ggatcaaatc ctcctgaaaa aggcgataga agccgtccat
    23221 agctcttatg ccccttactc tgcttttcac gtcggggccg cagtccgtct tgaaggcggc
    23281 gacatataca caggcagcaa ccaggaaaat tccgcttatc cgtcgggaat ctgcgccgag
    23341 cgggtagcca tatttaccgc atcctcccag catcccggcg aaaccattga aaccattgcc
    23401 atcgctgccc ataccgatag tttcgagttg tgcgatccgg ttaccccctg cggctcctgc
    23461 aggcaggtaa tggccgaata ccagaacctt tcgggaaaac caatcagggt gatcatgaaa
    23521 ggaatgtcgg agaggatatg gatggtcgaa ggggttgaaa acttgttgcc cctgatgttc
    23581 catgatgata aattaaaaaa ataatcagag atcaggcaaa tatggtctta tggaataaaa
    23641 ttaactggtt ctataagcct tgtataccgg atgtccggtt tattgaacag attgtggcat
    23701 gaccggatca gatttaaaaa aattaacaaa ttcttaaaat aatatatata gataataaat
    23761 aattcctact tttactccca tctaattaaa accaacgaac aacatgaaaa aatttactct
    23821 cgtcctggcg attctcgcca tcgcggccat tctgcctgtc aactcccaga atgttccgcg
    23881 aactatggtc gtactcgaaa tcggaaccgg aacctggtgc caatactgcc cgggagcagc
    23941 taacggggca gaccaactgg ttgatgaagg aaaatcggta gccgtaatcg aaaaccataa
    24001 cggcgactca tacgcaaata catattcgaa tgcaaggaat tcgtattatg ctcttgatgg
    24061 ttatcctacc gccaaatttg acgggacagt tacttatgtt ggcggccagc tctgccccac
    24121 agccaatata tattcggcat accttcccct gtatgaacaa aggatagccg tttcaagccc
    24181 cgtgaccgtg tgcataaccg gtacaaattc aggagacaac tatacggtaa atgtagccct
    24241 gaataaagtc ggcaccatta attcgagcaa tctgaaactt caccttgtgc tcaccgaatc
    24301 agatatacag caaagctggt cgagttgcat gaccgaattg aacttcgtaa accggctgat
    24361 ggttcccaat gaaaacggga ctgcagtcag ctttgcatcg ggcaattttc agacttttac
    24421 tttaaatttc acgaaaagtg ccgattgggt agctgcaaat tgcgagctgg tagctttcct
    24481 tcaggataat tcgaccaaag aaattttcaa cgccaacaaa gtaccgctca acgacatacc
    24541 ggcgactgcc atcacatccg ttgattttac ggccgacgtc accaccgggt gttcacctgt
    24601 cacggtccat tacactgacc agtcggtagg cgcttcgcaa tataactgga ctttccctgg
    24661 cggaactcct gccacgtcaa cgttgcaaaa tccgacggta gtctataata ctgccggaac
    24721 atacgatgta acccttgttg catcggatgg agtttgtgga aatgccgtaa caaaaactgg
    24781 gtatgtgact attgccagcg ccccggttgc acccggcacc ccggcaggaa caagcggttt
    24841 atgcagtgtt gcccccattc aaacctactc catttcctcc gttcccaatg ctgacacgta
    24901 tacctggagc ctttcgcccg ccgaatccgg cgtgctgacc ccaaacggta ccagttgcac
    24961 cgttgactgg aacgatacct ggtacggcac agccgaaata aaagtccagg ccacgaacag
    25021 ttgcggtgcc ggcccctggt cgcccgtcag gagcgttacc ctcagtgagg ttcccggccc
    25081 ctgtgcacca cccaccggac cgacggcctt atgcatgaat gcgtctgata cgcaatactt
    25141 cactacaggc gccactcctg ctacatatta tgtatggagc ctccagccca acgaagcagg
    25201 gaccctgatg caggatggcc tgtcggctac aattttctgg aacgatacct ttaccggaga
    25261 agctttcatt aaagtagaag gtatcaacgg aagttgtgaa ggaccttatt ccgatccgct
    25321 tgttgtaaca atttccgata atcctgcagc ccataatgta accggaggag gcacctattg
    25381 cgcccaggga ggcgccggag ttcccgtcgg gctcgacggc tcagaaaccg gtactgatta
    25441 taccctttat atcgacggag tggaatcgac caccataacc ggtacgggaa gcgcccttga
    25501 ttttggaaat cagactacgg caggaaccta tacgatcctt ggcaccttta ctgccggagg
    25561 atgcaacagc accatgaccg gctcggccat cgtggcatcc gatccccagg aacctctcgc
    25621 ccctgctaat ccggccgggc cttcggtagt ttacattacc tccactccca ccagcgatta
    25681 cacgacaaac ggcggcacct atgcaaccac ctattcctgg gaactgacac cggctgtggg
    25741 cggcactgtc accggcaacg gcacatcgtg caccgttgcc tgggatcttg gataccccgg
    25801 agactatacc attaaagtac agggtgtaaa ttcatgcggc ggcggttcct tctccatcga
    25861 atatcccgtt accgtttatc gcggagtagg aatgaatgac cctcaggaaa ctaaaccggt
    25921 gattgtaact cctaatccgg ccagcgacat tgtaacaatt atcccttcac agccattaac
    25981 tgctgacatc accgtgatta actccgttgg taaaaaagtg atagagattc atcaggcaaa
    26041 cctcggcgaa ggttataaac taggcatttc gaaactgttg cccggagtat actatatccg
    26101 cgtcagcaat tccgacatca gccagacaat gaaattagtg gtcaaataac accatcgatg
    26161 ttcattcatc aaaaacaggg tccggcgtca caccggatcc tgttttttat gctgttccgc
    26221 aattcctgac cagcgcattg caaggcaggc gcagctaagc cccaaaagta aaatagaggt
    26281 tggcaaagaa attccatacg gttacaatgc cgatggcaaa gagtttggcc acataaaagt
    26341 tccacttaaa cttcccgttc agggtccaga tcactaaagt gcttaatcca agcccgagca
    26401 ccgaaacaaa gaaaaactta ccgtattcgg tgattatctg gggattggtg ctgtgaaatg
    26461 tccagtacct gttcaggaaa taattactgg aggccgccac ggtaaatccg atagcattcg
    26521 caatatactt ctgaaccttt acgatttcct taaacagcca ggtaaatcca aaatcaacaa
    26581 aaactcctga aaatccgacg actccgaatt tcaaaaattt tatgatcagt gttttattaa
    26641 aataactgaa catcgtgaat atgcgattat taaagttgaa cgataagtac tatcttttcc
    26701 ccgtcgcgga ctatttcggc tgatgttacc tgacctggtt tcagcaagcc aagtcgcgcc
    26761 atatagtcgt aaatattggt aaccggctga ccattcaggg aaacaatacg gtcgcctttc
    26821 ctgatccccg ccttttcggc cgggcctccc tttctgacgc catccacgcc caaaccgtcg
    26881 tttgccgtgg aaaccacatc cggtatgatt ccgagggtaa ccttaaaatt ccttcgtgcc
    26941 atctcaccct gttttgggcc cgtttctcgg taagtcagcg cctggtcacg gcaggagaga
    27001 tcaaccgtca ggctgtaaac aaaatcaagg attgaagcca ttccggaata atttatctta
    27061 tctgcatcat ctgcaggcgt atgataatct tcatgcgccc ctgaagtgaa atacacgacc
    27121 gggatatttt cggaataaaa cgaggcatgg tccgacgggc cataaccatc gggagaacgg
    27181 cttatggaga ggccgccgtt ttgcgagagc aaactgagga tagtatccat ttcagccgca
    27241 gtgccagttc cgccgacaga aacagccttt gtcaaggtat ccaggcgccc gatcatatca
    27301 aggttaatca tcgctttgat ccggctgagg ggaacgggag gattttttac aaattctttg
    27361 gaacccagca accccatctc ttcgccggca aaagcaacca taatcaccga cctttttaac
    27421 ttttgcctgt tttccgacag tttccgggca agctcaatca atccggcgac tccagaggca
    27481 ttatcatcgg cgccgttatg cactgccagg gtatcgggcc atcgtgaacc ggaaccgctg
    27541 ccgcccatgc ccaggtgatc atgatgagcg ccaatcacga tatattcgtt gcaaagcaaa
    27601 ctgtcggccc ccggaatcat cgccacaatg ttggcggtgg gcgcttcctc caattccata
    27661 ccggcgacgc cgtcaagcat caccggcata tacatcggcg aaggcctgtg ggtttcggtg
    27721 atccttttta cgagcccggc tacactgtcg ttcatggacg caagcatacg atcggcaagg
    27781 cttcttgtga tactcaccac caaaataccg gcattagctg ttgtccggtc gtattgcgga
    27841 ttcatcggga tatcgcgcgc ttcaatccca gcaggagaga ccagcagtac cccggcagcc
    27901 cctttgtctt ttgccgtaag cgcctttagg cgttcctttt caaatcctgc aaacaaactg
    27961 tttggcctgt cgggctcagg atcgccgcgg agcaccatta cccatttacc gtttacatca
    28021 atgccggcat agtcgttcca gcgcaaggag tcgagattga gatcgaaacc aaagccggca
    28081 aaaaccaccg gcgatttcaa ttttccgttt cccgtaaaag ataatgggct gaaatcttta
    28141 tacaatacgg cggagtaatc agccaattgt aaggtattct cagccccggg atgcaccttc
    28201 gtcaccaccc tgaattcctg ccttccgtca ttacagagca tcctaagccc tgcgccggaa
    28261 aagcaatctg caatatactt ctccgctttt tgatcgcctc cgctcccggg gtatcgcccc
    28321 gacaacgaat cagcagccag cacgccaacg tgcaatcgaa ggctttcggc tgaaatgagg
    28381 ctattgtcct gggaaaatcc ttgtgccgcc atcaggacca acatgaccaa catgaagcag
    28441 gcattccgga tcattaaagg aatttttatt gaaataattg gcaaaaataa aggttttaac
    28501 gctatccgga agaaaaacct ttgcaacaag atcataaaaa aacggaaatc aatccgcgaa
    28561 agttgcggct tccgcttcag taagcggcta tccgtcacca ggatcagatg ggttcagata
    28621 ccgtccggat caggatgttg ataaatatgt tgataaaaag aataaattcc tcctgaacct
    28681 taataaagat tagctgaact ttacctatga caatcctgct ttgcaggatg gcttgtcgac
    28741 gactcccttt actactcctt cttggcagct atatttaccg ctgacgccct gccctcaaac
    28801 gaaaccccgc tgccacgacg gggttttcat tttgtcttcc gctgattgat gttacaggat
    28861 gagagtcgcc cgtcacatga atggcttact gacaggaaca gctaatagga gtttcgggct
    28921 tgtcactacc cacatgattc aggaaacgcc ccctgttccg gcctaacgtc ctggcactct
    28981 ccgtttttgg tatatcttat tgagccggca tattaatatt taatctgttg aaaaattcaa
    29041 actttaatat tttcctgtat ctttagcgta ttgattttta ctgttttgca attcccgcct
    29101 tgaaagataa acatccgggt ctcatcctgg cgggcattct aaatttaatt caaagttgca
    29161 ctttacgacc aggaattgtt acatctgata aattctgtct aattttgaac acttaaagcc
    29221 gtccatgcat gctgacgcgt cttaacaagc agttgatttt ccgggttatc ctgccctctg
    29281 cagcaatatt aatcattgcc ggggtactgc tttcagcctt ggaggattta ccggcggcag
    29341 gcgctgttct cattctgaca ggtctgatgg ccgggatttc cgggatactt atcgttcacc
    29401 ggatactgag gcctgtcgcc gaagtggaaa aagccgttga atcgctcgaa aaccggaaat
    29461 gcctttcgga caaagctgtc ccggttcaac acgatgaccc tttgctgcaa tcgttatatc
    29521 atatttccga aaggatagaa ctgcaggaga agaaaattca ggatgaaaag ctgcgccgtt
    29581 tgcgttcggt tatcgacggg caggacagcg aaaggcaacg actggcgcga gaattgcacg
    29641 acggactggg acaatcgctg atagccgcca ggctccgact cgaatcgtgc gaaaaaccgg
    29701 ggatttccca tgtacgctcc gccgtcgatg cctcaaaaaa tatgatagac caggctattg
    29761 aggaagtgag gcggatcagc aatgcattac agcctgccgc cctggatgaa ttcggcctgg
    29821 aaaccgcgct tcgcacccgc gtcatggaga tggccgccat tgccggcatc aaagcatcgt
    29881 tcgaatgcaa tggcagcatt gaaaggcttg ctaaaaaatc aaaaatatac ctgtaccgca
    29941 tcgcccagga agcaataacc aatattatca aacatgcccg cgcaaccgaa atggcggtga
    30001 caattgcccg cgaagatcag ctcgtaaggc tcacagttgc cgataacgga aagggtttta
    30061 tgattgatcc caaaagttat gcacaccgca acgggataca gaatatgcgt gaacgcgcat
    30121 cgctcctttc gggaaatttc attgttgaat cggcgccgga tgccggaacc cggattattg
    30181 taacaatacc ttatacacga aatgacggat acgataaaaa tactcctggt tgacgaccat
    30241 catcttgtca ggaccggaat tgccaacctg ctttcagggg aaccggaaat agaagttgca
    30301 ggggaagcgg cagatgccgg tgaaatgctc gaactgctca aggtacggga agccgacctc
    30361 gccatcctgg atattgcatt gcccgacgta tcgggaattg aactggcaag gcaaatggcc
    30421 aaagagttcc cgaaggtgaa agtaatcttt ctgtcgatgt acacctccga agagtttgtt
    30481 ttcaatgcca ttaattcagg ggctaaaggg tatctgccga aaaacatttc cagggcagag
    30541 ctcctcgacg ccgtcagggc cgtttaccgc ggcgaagagt acttcgctga atccatctcc
    30601 aataccatac tgaaaagcta tatcaaaaag gcgaaatcgg gcaacctgga aaacctgcgt
    30661 aacgaaagcc agctttcgaa acgcgaagtg gaggtactca aatggttcgc gcagggatta
    30721 acaaaccagg aaatcgccga taagctcttt atcagtatcc gcacggtgga atcgcacaag
    30781 aatcatatca tgcaacgcct cgaattaaaa actaccgtcg acctgataaa gtttgccatc
    30841 cgcaataacc tcgtaaattt gtagcctctt tcctctaatg acatcaaccc gatacctgtc
    30901 accatgaaaa aagcagccat cctcctcctt ttatttatgc catttatcgt caatgcacag
    30961 gaaatgaacc ggaaaggcgc cgatccgaag aaaaacaatg agatcctgct gggttactgc
    31021 aaccgggaag gtttcagcgc catcaacagc aacttcgatt ctgcctacag ggccggttac
    31081 gatatttatc agcctgacga ttcaacgctg aaccttctga aaccggcatt aaaggatgtt
    31141 tcggtaaagc ttatcctggg aacatggtgc ggcgacagca aggaatgggt cccccggttt
    31201 tttaaagtga tggacctggc cggtttcaac tacgataacc tggaaatgat cgcagtggac
    31261 cgtgagaaaa aagcaggcga tctgaatatt gaagacctga aagtcgaccg ggtgcctacc
    31321 ttcattttct ataaaaaggg gaaagaaaca ggccgtatca ttgaagcgcc ctacgatctg
    31381 atagaaaagg atatgctgaa gatactccgg gacagataag cgccttacgg ttcttcggca
    31441 aaagtgttaa cccaaaacaa cagacgggat gagagtattt gaaaaaatcg aagatctgcg
    31501 acaatatctt gccggattaa aagaatcggg aaaaaccatt ggtttcatac cgacaatggg
    31561 agccttgcac gacgggcacc tgcaactggt gcggaccagc atttcacaaa acgacattac
    31621 aacggtcagt atattcgtta atcctattca gttcaacaat ccggaagacc tggccggcta
    31681 cccgcggaat ctctccgccg acctggagaa actggaatcg gcaggttgcg atatcgtgtt
    31741 tacgccctcc gccgaagaga tgtatcctga acccgttact aatgaaatgg atttcgggca
    31801 actggataaa gtgatggaag ggtttttccg tcccggccac tttcgcggcg tgggcatcgt
    31861 ggttaagaaa cttctcgaca tcaccgggcc tcaccgcgct tatttcggtg aaaaggatta
    31921 ccagcaactg gccatcatcc ggcacatggt gaagtcgctg tcgatgccgg ttgagattgt
    31981 tccctgcgcc atcgttcgtg aagccgacgg gctggccctg agctcgcgca acaagcgcct
    32041 tacccctgaa cagcgcgccg ccgcccccgg aatttaccgg gcgctgctga aggcaaaaga
    32101 aaattacaac tggtttaccc ccgacggaac agaaagcctg gcgatcaccg aaatagagca
    32161 aaaccctgag ctgaaggctg aatatgccac cgtgtgcgat gcaaccacgc tgcagccttt
    32221 cgaagactgg atggatgcgg aacatgccgt gttatgtgtg gcggtttatg ccggccaggt
    32281 acggctgatc gacaatattt tgctctatta aaccggggcc ggaagaaatc ctgaaacggc
    32341 aggccgcccg ggttgccggg ttgccggagc tgctggagcc tgaatggccc tgcatgcagc
    32401 atactgcgtt aattgacatg tgaagcgcta tttctttgta cttttgcagc atgatgacaa
    32461 ttgagatttt aaaatcaaaa atacaccggg ctaccgtcac acaggctaac ctgaactata
    32521 tcggttcgat cagcatcgac gaaaacctga tggatgcagc caatctcatt gaatttgaga
    32581 aagtgcaggt agtaaacatt aacaacggcg aaaggctcga aacttatgtg attaaagccg
    32641 aacgcggatc gggagtaatc tcgctcaacg gcgcagccgc ccgtaaagct tcggttggcg
    32701 acctggtgat cattgccggt tttgcccaaa tggagttcgg ggaagcaaaa aagttcaggc
    32761 ctgccattgt tttccctgat gccaataacc aaatcagcta aactatattg aaaaataagc
    32821 tcaaaaccat cgcccgtttc ctgctgttcc tgggcatcgg catattcttt atctggctgt
    32881 tcctgcatta cctgacgccg gaagaaaaaa aggaaatcat ccgttcgttc ggcgacgccc
    32941 attacggcat catcggcttt gccatcctgc tgggtgtggg ggcgcattac ctcagggccg
    33001 tccggtggag gatgctgcta aagcccctgg gatatgaaac gggcaaactg aatacctttt
    33061 ttgccgttat gatcggttac ctcgccaacc ttgccctccc ccgcctggga gaagtgagcc
    33121 gttgcgtgat cctgagccgc tatgaaaaag tgccttttaa caattccttc ggcacggtgg
    33181 ttaccgaacg ggccatcgac ctggtcgtct ttctcatcct cttctttata aatctcgcat
    33241 tacaattcaa caagttatat acttacatcg accagaaaat ttaccagccc attgaagcaa
    33301 aattcaacct caccttcagc ctggcaggtt cattcaccta catcgccatt gcttccctgg
    33361 ttattttggc agccctcttg tttgccttcc gccgtcccct gctgaggata aaattcatca
    33421 ataagatctg gggccttatc aaaggattct gggatgggat gaaatccatt atctatctcc
    33481 ggaaaccgct gctctttgtt atttattcgc tggggatatg ggcattgtac ctgctgatgg
    33541 cctacctggt attcttcagc ctgcccgaaa ccagccacct gggccttgat gcaggcctcg
    33601 ccaccctggt attcgggtct atcggtatca tgctggtgca gggcggcatc gggatttacc
    33661 cggccatcgt ggccgaaaca ctggtactgt attccgtatc gaacatcaaa ggctatgccc
    33721 tgggatggct gatctggagc tcgcaaacaa ttaccatcat cctgcttggg atcctctcgt
    33781 ttatcctgtt gcctgttttc aacaaaaacg taagccatga aaaagcttga gtatgtaaaa
    33841 gcgaaaattt tcgcccgcga cagtgtggac tttagccggt tgctggccat catgaaattc
    33901 caggacaaga agatcgtttt cacaaacggc tgtttcgaca ttctgcacct ggggcatatt
    33961 gattacctgg caaaagccgc cgaccagggc gatgtgctga ttgttggact taacaccgac
    34021 gaatcggtcc gcaggattaa aggcaacaac cgccccatac aggatgaagt ttcgagggcc
    34081 atgacgctgg catcgctccg gtttgtcgat tacgtggtat tttttggcga agacaccccc
    34141 tacgaactta ttaaaaccgt ccagccccat gtgctggtaa aaggcgccga ttacaaaccc
    34201 gctgaaattg taggatacga catcgtaaag gcccgcaaag gcaaagtggt aaccctcgat
    34261 tatcttgaag gctattctac aacggccatc gaaaataaga tcctgaaccg gtaaaaggac
    34321 tggctgggcc cgggaattga gcgtatgttc tcttcccgga tccgatattg taagcctggt
    34381 agacaggctg attccctgca tcagaaaaat ttttattttt ttctttccac tttgtcttga
    34441 cacaaagtgg agcaaaagtc aagacttaat aaaaattcct ggttttgtaa caaacttttc
    34501 cttccccgcg atacaagccg cgcccgcgca cgtaccttcg cctgcttctg tatccctgcc
    34561 tgccggcagg caggacttag cgcaaggcta tacgcacaaa aagtttgtaa caaaaactgg
    34621 cggaattttt atcaggtcag tctattccgt atacaattat cgattgggga tgttttgttt
    34681 ccatttataa ttttgcattg agtggaaccg cattggctgt tgcgcgtgaa gaaaactgcg
    34741 taacggagcg ttaagaaaca aaaactgttt aagcgaagcg agtttttttg ttttagcata
    34801 gtgaagtacg gcattgctta gcccacatat tcaagcttcc tgttcccggc aaccggggcg
    34861 caaaaaaagc catgccgtgg ttcgcggcat ggcttctgaa tggtgttcgt tttttccgga
    34921 ccccttcccg tattaacggg cggatactat aaatttcctg acggtctgtt tcccgtcgtt
    34981 gccggtaaaa caaatcaaat aaataccgtc ggccagggca gatgttacaa tccttccttt
    35041 caggccggta agggcggttt cccggtaaag cattctcccg gcctggtcgg cgatcttaat
    35101 ttccttaacg ggagatgcag cctcccagta aagcaactca cgggccggat tcgggtatag
    35161 cctgaaatcg ggcgattccg tcatctccac ccctgtcccg atgcaattca atgaatcgat
    35221 gttcgaaata gcggaagttc cttccgtgta atacgccatc acataataat cataacagtc
    35281 tgaggccagg gaatggtctt cataccatgt tttggtggtt tctgcaatat cctcccatgg
    35341 accggggacg gcagtatgca ctcttctgaa aatatggtac gacagcaaat cgtcggtgcc
    35401 ggctgcgctt ttcaggttca ggctgtcaac gggacaattc cactccaggc tgatggcgcc
    35461 gggattggca tttgaaggag tcgctgtaag gttgtagacc gggtataata agtacttgat
    35521 attaagttca atattatcaa taaaccagtt ttttatggca ttgctttgct caccactcgc
    35581 agcaactttg aaatataaca tattcgccgt ctgaggtata tcaaagtcat aagaataatg
    35641 tgtccattga aaatctccct ggtttgtgaa cgaagcgact gtgcgccaat tatcatcatt
    35701 atatttataa aaaaccgtca attcttcagt acctgtttca aggctgtcgt cgagcgccag
    35761 atcgaactgc accgttaata aatgaggtgt tccggttgac agagaaaaac ttctcgtaaa
    35821 aatttccaat gagctctcat aatctgtaag ttccgggaca ctgttaaatt tcgcacaagg
    35881 ataaggatta ccatatataa tatcaattac ccaattatca ccgtgagccc attggttaag
    35941 atcaaaagat ccgcaagtcc aatcttctat caatggcagt gagcagcagt agaaaatatc
    36001 ggcacaagcc gggccggcgg caaaagattc gccggtttgc ccggaatagc ccagcggttc
    36061 caggttgtaa acggccgaaa gcttgtaaca atacatgccc tgatccagat tcaggtgcca
    36121 gtaagttgta tctaaagacg acaacgtatc gatgagcgtg ccgttacaat acaggaggta
    36181 gtacatcacc cacggcccca ggccggtgtt cccgaaatca ggagagtccc agtcaagaat
    36241 gaccgcgtta tcattaacac tagcctgcag gtttcccgga taaggcaggc agcggttggt
    36301 aaacatcagg gaatcatgcg gcgaaacgcc attggcataa acggcagcaa tttcaacttt
    36361 gtactgctgc ccgaacacaa gggattccgg gttgatctgg tatgcatttg aatccgatat
    36421 ggccagaaag gcatcgttga gccacaggca ataatggtcg agggtggtaa catacgccgt
    36481 gatgttcaca tcgtcaaccg cccatcccga ggcctgctgc ccgttgtcag cggcatgaaa
    36541 acggatgatg gcggaggcgc agccacccgg accagcattt tgagataagt caatggcaat
    36601 gcgaccccat gtttcataat aaggcgcagc agccggctgc agggtatcca tcacctgcca
    36661 gctcgccccg ttatcggtgc tcagttcgat atacgccgcc tggttaaatg ctccggtgta
    36721 acaggtatta aaagtgagga cagccgaatc ggtgagggtg aggtcaacag ccggcgttac
    36781 caggaaatca caacagccgt tgttctgctg accggcatta aaatcatttg ttgccgcata
    36841 ctgcgaatga ggcccgatat aaaaattcag gctgttggca ttttctgtca cgtaccatcc
    36901 ggaattcccg gctgacgagg tcgtccagcc ggccggtaag gcatcgccgt cttcaaaatc
    36961 ttcatttaac aattctacgg acggccaatc ccaggtcatt aaagccctca ggctgtcgac
    37021 cctgaaattc acgggagggt aaagactgtc gttgctgatg ataccacaaa aggctgaagc
    37081 ggataatgac agagagaata aaacggcaaa cagaagccgc agaaaggtgt aatgtttttt
    37141 catatcagaa atttttaaat gttaattcaa acaagtgagc cgataataaa ccgatacaat
    37201 aagatgacaa tttgaaacgg atttaccact atccgggaga atattttcta cggaatccaa
    37261 atattctgac cagggatgtc agccggattc ggtgaattca taattttata acggagaacg
    37321 gagaacggag cggggagcag ggagcaggga gcgaggagcc aggagccggg agtaaggagc
    37381 acggagaacg gcactttagg cactaataac gcaaaacgca taacgcagaa cgcaaaacgg
    37441 agaacggaga agggagaacg gagaacggag cgaggagcac ggagaagttt aggcactaat
    37501 aacgcaaaac gcagaacgca gaatgaagaa tgaagaacgg agaagggaag gagcatagcc
    37561 tgccgggcgt aagcctgcgg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37681 ntaacggcta acggctaatg gctaacagcc ttatgttaaa acgcaaaaca cagaacgcag
    37741 aatgaagaac ggagaaggga aggagcatag cctgccgggc gtaagcctgc gggtggcagg
    37801 cagaagctaa cggctaacgg ctaatggcta acagccttat gttaaaacgc aaaacacaga
    37861 acacaaaaca caaaacacag aacgcaaaac gcaaaacgcc aagagccaag agccaagagc
    37921 taaaggctaa aacgcaaaac acagaactca gaaataagaa ttcaaacggg catattacat
    37981 ggctaaaatc aagacaagtt ttttctgccg gaactgcggg gcgcagtcat caaaatggat
    38041 ggggcggtgc agttcgtgcg gtgaatggaa taccttcgtg gaagaggtgg ttcagcatgg
    38101 cgacggcgga gcaaaaagcg gcggtaattt caaagctacg gaaagccgcc cgaagctcat
    38161 ccatgatatt gcccccggca atgaacaacg gaccagtacc ggcaacggcg agctcgaccg
    38221 ggtgctgggc ggcgggctgg ttgatggttc cctcgtgctg ataggcggcg aaccgggcat
    38281 cggcaaatcg acgcttatgc tccaggtggc gctgcaaatt aagaaccggc gggttttata
    38341 catttcgggc gaagaaagcg aacagcagat ccgtatgcgc gccgaacgca tcggatacaa
    38401 cggcaacgaa tgctttatcc ttaccgaaac caatacgcag agcatcttcc agcacctcga
    38461 agccataaag cccggactgg ttgttaccga ttcaatccag accctgcaga ccaatattat
    38521 agaatccacc gccggcagca tttcccagat ccgtgaaacc gccgccgaga tgcagcggta
    38581 cgccaaaaca accggcgtgc cggtcttcct ggtcgggcat attaccaagg acggcagcct
    38641 ggcagggccg aaaatcctgg aacacatggt agataccgta ttgcagttcg aaggcgaccg
    38701 caattacggt taccgtatcc tccgcgccgt aaaaaaccgt tttggttccg cttcggagct
    38761 gggcatcttc gagatgcgcg acaacggcct ccgcgaagtg tcgaatcctt ccgaaatatt
    38821 gctttcgcag cgggaccagg aagtaagcgg cgtcgctatt gcctccaccg tcgaagggtt
    38881 gcggcctatc cttatcgaaa tacaggcgct ggtaagttcg gcagcctacg gcacgcccca
    38941 gcgttcggcc accggtttcg atatccgtcg cctgaacatg ctgctggcgg tcctggaaaa
    39001 aagaagcggt ttcaggctgg gcgccaagga tgttttcctg aatatcgccg gcggcatccg
    39061 cgtcgacgac cccgccatcg acctggcggt catcagcgct gtactgtcgt cgaacggcga
    39121 tataccggtc gactccaaga cgtgctttgc cggcgaagta gggctttcgg gcgaaatccg
    39181 cccggtaagc cgcatcgagc aacgcatagc ggaagccgaa aagctgggat acgaaaccat
    39241 cgtgatctcg aaatacaata ccaaaccttc aaagcttgcc gcccggcaaa tcagcgtggt
    39301 gaccgtaacc aaagtggaag aggtgttcag gcttcttttc agataagcgg agtcacctcc
    39361 ggagaagcaa ttttctatct gagaaattcc gaagctcttc tgaaaacttc tttaaaggcc
    39421 gcatccacac tggggatgat gcctttttcc ccgacgggaa acggtatttc atgcgaatat
    39481 tcataaggga aatccatcac ttcgatgctg gaaccgcccc ccagggcttc gagggtccct
    39541 gccgccggta tcactttatc gtttttcagc actacagacg aaatctggtt tttcagtttc
    39601 gaaaaaacac cttcccggaa tgccttcagc ttgccggacg aaagcatggc gctgaaagcc
    39661 atccccaact gtgtgctctt caggtactct gcaaatcccg gatgattgtc cgatttcgtg
    39721 cccagatcct ggatataata cttcctcagg caatcgaaag catttttatc catgataagt
    39781 ttcgaggtgc cgtccatctg gtcgaaaaag gccccgccgc aaaaaatgaa cagccggcta
    39841 tcagtgaaca ggttgccggg attagccagc atgacgatct gggccaggaa tgccccgatg
    39901 gaatatgcaa acaggtcgat atgcgcacct ttttcgaaaa gcgggtgatt gccggccgag
    39961 atctgcaaga ccagttgttc aaggtcgccg gcgctttgca cgccggaagt cacaaaccgc
    40021 accggatttt ctgacagcct ttcgctcagc gccaggttgg cgaaggaagc catggggaca
    40081 tgcccgagat ggatatttcg ttccgtcagc aacggcgtca tgatccgggg attgctccat
    40141 gccagcggcg accggttcat atgataggcg atgggaaaaa ggatcacggg acgtcccgtt
    40201 ttttcggcca gaaaataagc ccataccaga tacttcgtcc agcttcgttc gttcagcccg
    40261 tgaagcagca ctattgcctg cccgaattta tctccggaat ccggaaatat taccgggtat
    40321 gtgaacgact ggttttcgcg gatcgattcg ccggcttccg aaaatatccc ggatgtgctg
    40381 gtttgattat taaaatcgtc ggtcggacac cccggggagt gaaatgaaaa ggtacggcac
    40441 gatatatggc tgtccgaaaa ctttatttca tctgccgaaa ggctgaactg tgatttcagg
    40501 ttgtgatatg ttgaagtata ttccatggtc ggcgtcataa aagtgacgat gcaaagtaac
    40561 tgcggccctg aacgcctttc aattaaaagg gttacagccc gctcccatgg gatgaaaagt
    40621 cgcatggagg ggtaaaatcc ggaaatatgg ggtaaaattc cggatgcagg aattcgcgat
    40681 ttaagaatat tatttgccgg aatggactaa atttgacatt ccaaactccg gatgatgcct
    40741 gatttcaaca gcccgatacc gcatttcctt acccataagc ggaacattgt aaagctggtg
    40801 ctctttaccg ctgcttttgc actggttttt atcaatgttt attcgccttt cggcgtcgac
    40861 gtgtggtttc atgtcaccaa gtggcaattg ctgttctatt ccagcctcgt catcctaacc
    40921 ggcgtcctgg tgatcgttat cagcaggatc atcatgtatt acgtcagcag gaaaaaagac
    40981 atcaactact ggcagtattt gggatgggtg cttgccgaaa ttgctttcat gtcgctgttt
    41041 tatacctttt atgtaaagta catcctgcac gacaggcgct atttccccga tttatggaag
    41101 gcttcgatcc agaataccgc gctggtgctg ttgctgcctt attcggtttc gtggctgtac
    41161 ttttcgtatc tggataaaaa gtccaaactg gccgaactgg cccaggggca gcccgtgaac
    41221 gacacctcaa aacagatgat gccgtttgcc gacgaaaagg gggtgcttcg gttttcggtc
    41281 aagaaagaga acctccttta cctcgaagca tcggataatt atgtgaatat ctattacctg
    41341 aacggcgaga aagtgtcgcg gtttatcctc aggaattcgc tgcgcacgct ggaagaagaa
    41401 ttaaaatcga ccgatatagt acgttgtcac cgttcctaca tggtgaacag cgaaaaagtg
    41461 aaagtgatac gccgcgacaa ggacggcgtg cggcttgaac tggatgcggc ttcagccatg
    41521 gatatccccg ttacgaaaac ctacctcgaa aatgtgatga gcgcgttctc gcataccgcg
    41581 ccgggaaata aagaataatt ttaataaccg gcatcctgcc ggtaacaacc tgcgcccgct
    41641 gaccagcccg gtaccgctat tttaccacca tcacttttcc gccggcaatg gcctgattcc
    41701 catgtgcctt tatcatgcac cagaccctga aggttttgaa aaccttcagg gtctaagggc
    41761 tatgatgcag gcagtaatat tgcctgttcg gcagaggtta attaccggtt tcaccatttc
    41821 cgtcattaaa ggacgaaagt ttaataatgg caggcttttc ggtcataatt atcttaacac
    41881 catcaccttg ccttgtgcca caaatttccc cttttgggta aggtgcaata catacattcc
    41941 ttcgggcaat ttactgacat ttactacatg gttgcgttcc gaagcgtcaa atacatcggt
    42001 attaacgatt ttaccgctaa tattaataag cgacagttcc acctcgcctt ttaacgggcg
    42061 gtactgttgc tgattcacgg atttactttt ggttgtagtt aacgaaaatt ccggcatttc
    42121 tacagtggtg tatgacgagg caggattggg gtacaccttt agctggagta tatcgcttgt
    42181 gcctttcacc tccggcaggg cggtaaacac aaggcagttg ggaggcaccg gaatggtatc
    42241 gcttactatg gggtgagggc acaggctgtc gtaggtgcgg ggggcggtgt atatgctatc
    42301 gtactccagg ttggtgttga atttgcttaa acttatccaa tcataaggga aagcgccatt
    42361 accagacata tcaataccca gtacaactat tttgttatca aaagttttaa ttgacatcat
    42421 tggaatattg tgctcttgct tatagataca tttataatta ttccctaaag tatcaataag
    42481 ttgaagtgac caccaattgt cagtgtatgt aaccccaaat tgggtgttaa gaagaagcgt
    42541 tgtatcgtta aatgacctga tataatgagg tttacaagct attgagccgg gatgattata
    42601 gacgttgaaa tatgatatgg aatctccatc tttgtcaatt ttaaaaacat aagggtgccc
    42661 caacccagga tccatgatgc ctccgctgta ataagaatta ttgcgaccct ccgtaacagc
    42721 ataaacttca gcaggggtac cgccccaata taaattccat tttctgtccc ataagaggtt
    42781 tccgttaata tccactttga accagaaagg catatcgctt tgagccccgg ggtcaggatg
    42841 aaaaatagta tcattcacca tcccgcaaac caccaggcag gtatcggttg ttaaggtagt
    42901 cctcaattgt agctgcgaac cgggatacca gtcaggattg ttgtaaacat aataattgag
    42961 ccattccaca ttgcccgatg ctgataattt tgcaatgctc catattcgtc cgtccggtac
    43021 aaaatcctcg aacatgcggt gcacaaaaaa acctccatcg ggcgtttcaa aaatccctct
    43081 tgaaataatg gtcgggtaat ggtaaatatt gtcggggagt atggtgcacc actgtggttc
    43141 ggcacaggca ttgaatttta ttacgtacga atcaatatca ttaccctgag tattgtagtg
    43201 cccacctgca ataaaccctc catcggaagt tgcgtttacc gagccaaaag ttgtttcgcg
    43261 ggcgccatca atcacctttg tccatagttt gtttccgttg atatcagttt ttagaatata
    43321 cccaaaatag ggagattgca ggttcctgat caggccaccc gtaaggtacc ctttatcgta
    43381 ggtttcggta acttcgttgg cccaggagcc atagggcagg gtatagtttt ttttccacac
    43441 atattgggcg ctgcaaaatg caggtaaaat caataacagc aaacttatat agtgaatcac
    43501 agttttcatt attcatacta ttgagaaacc aaagcctgct tgccacagca ggctttggta
    43561 gagaatttaa atttatctgg atactgtaag ttttcgcgaa gaaattgatt tgttcccgca
    43621 cgaaagtgta actgtgtaag cacccggctt gaacccggtt aaatcaataa ccaactgatc
    43681 atttgctttc actatattct gtttccacac tatgtttccg ccaatgttat atattgttag
    43741 cgctgcattg ccctgcttgg aaaggatatt gtattcggcg atggtatatg tgcaggccgg
    43801 attgggcagc aaacgtaaac tttcaacgct ttcggcgcct ttacttatga atgtttttga
    43861 ttctgccttt attgatttgg tcacttcaga gtcgttgatc ggttcctggt aactgagtaa
    43921 gcctgaggca acgagcatat cacgggcggc aacgcaggca gtattgtcgc cactttcgta
    43981 aagggcgaat aaaggtatgg catgaaggct gtcgaggcca aagggttgaa tgttgtcggc
    44041 agccagcccc tgcagtatcc cgatgagtgt gaagtaatcg gcggcctgtt gttcctgtga
    44101 ggcattaagg gccgggttgc cttgtagtgc tgtcacagcc tgcacagcag cagcataatt
    44161 actttcgccc atcaaaatat cggcaagcag gaagcctgcc tcgggcgttt gctccgcttg
    44221 taagaatgcc ttcaactcct gtggtgcatt agggttaatg gtgtcggtga ggtaggcgct
    44281 tacaagtcgg ttgtaaaggt aactttcctg ttgcttccac ccgtcgcgta cggcttccag
    44341 tttctcacgg ccacccacaa cgttaatacc ggcggacact tcgtcctgca tataatcggg
    44401 catggggttc cagcgctggt caagcatatc cattatttca ctgttttttg ctgcctgcgg
    44461 attttcaacc agcacatccc ggaccatggc gttgggcaac acgttttcct gcataatagc
    44521 ggttttcatc acggtgtcgc tcaaataggg cgattcggaa agtaactggt cgcgcagttc
    44581 aagtgcttcg ggcggggacg aacccaatac atccatagta aggttttcgg tgcttccgcc
    44641 atcaacaagt acctgtaatt gtaaacctgc agtttctgcg cctgtttcag attcctcttt
    44701 ttgagcgatc aggtcttcga tagcaggagc accggaagaa atccacgatt tacaaataaa
    44761 agccttattg taagttgtat tatcattctg acttcggata atattataaa caggatccgg
    44821 tttaacttta atgtttagtg gatcagtgta ctggtggaaa taataattag atacattccc
    44881 cgataagttg cggatattgt aagcgcttgt agaaagcgta aatgtattac ctgctgcaag
    44941 ggtatctttt tgtgtacttg gatttcctgc tgatggcaaa ccctgataca atgaaatacc
    45001 ggtatataca gatggtgtag ttgccaatac gtcaatatcc gttccgttag cgctgaaatc
    45061 attgcatttg aaacaaagcc ctgtggcgcc acccgatgcc cggttgttac cgtcggcaac
    45121 agcggcacgg ctcaacctgg tgaaagtgtt gcgatacaca tagttttgtg ttgttcccga
    45181 gttataaata taaaggccta tctgccccgt agtattggca atctcgcttt cgaaaacatt
    45241 gttctcaaga tggtaaccag tgcatccttc caggtataac ccgtatttat caattccatt
    45301 gagtgtatac tcgggtatct ggaaatcatt attaaggata tcaaaatatc cgcaattact
    45361 gaaataagcg cctctgttgt tgccaatgaa atcagtgtat gtaatttttg tttttgcacc
    45421 ggcaaaatta tattgagcat aaataccccg gttcaggtta ttgaactgat tgcgtttaat
    45481 atattcgtca ggacagggta aaacattgga actacagact ccgtttaaag tgaagtcact
    45541 accataggac ttaattccca ctccgatggg agcgtcagaa taaacattcc tgaaaataca
    45601 cgaagagaat cgcattccat acattgctct caaatttacc attatactgt tatccgaata
    45661 tgttccaaca aagtcctgat tgacttcaaa agtacacccg gtaaaatggc taaaactagc
    45721 gtaatcgtac ctttcaaaat ctatagcgac atcattgttc aggaagtcag aatttaaagc
    45781 ccaaaccatc ccgcctccat attcagatgt tcttttaata tagatagctt tctcggcgtt
    45841 ttcaattgta gcgtttgtaa gcattgccaa cccttgatat gtaggagatt gggtggatat
    45901 tgaattatct ccataaaccg taattccttt ccatcggtgg gtagttgagt tacatgaatt
    45961 agttatgaca gaattattta catatagttt tccgccaggc atgaccgtga tgccggtgtt
    46021 gtctatcata tccagttcca tatcatccag ggttaatgat gctccggaat taattataat
    46081 atttccacga gtccgattac tgttccaggt aatattatca gaaatattct ctgttacaga
    46141 ataactagct aatgtaacag cagtatacgc atcaactatc ccatagccaa gctcagaagt
    46201 ccagcctggc gatggaataa aaccagttaa ttcgtcccat accctgcaac ttttatacag
    46261 gatttcttta atctcatcag gtgttaaaca aggatttaca gcaatcatga gagatgcaat
    46321 tcctgaagca aatgctgttg aaaatgaagt gcctgaaccc gaagaaaatt gcaggtgatt
    46381 tggatacgga acatttccat cgccccaatt ggccataata tgatcgcctg gaagcgtaat
    46441 ttctgcgcct gtcccgtaat tggaagtaca aataggatac aaagagttaa taagacgcgc
    46501 tgaatagtaa tcactagcac cgcatgatat gacatgttca tgtgaagccg gataaaatac
    46561 atatgaatag cccgcatcgc cagcagatgc aaacattact ataccggctt catgagctgt
    46621 attgattgca ttattcaatt gaatcatgta ctcttctccg ccggcattga tccaggcttc
    46681 aacaggagcc ccataactca tattgataat tttcgcttga ttatcaattg catattcaat
    46741 cgcttctaaa aaacccagaa catcaaaaag taaatcattt gttacaggat cccggccttg
    46801 gttacgtaac atcatttttg ttaccggtgc actattccaa ccacctgcta ttccccatgt
    46861 tccataattg ttatttgttc ttgcaccaat acacccactc atcgctgtcc catgcgaggg
    46921 ataattaatc atcggatcag gatcagcatc atcatctatg aaatcccaac cttgggcttc
    46981 tgattgattc agatcaatat catcgtaatc acgaccgtca tcaataatag ctataattgt
    47041 ttgcggatta ccggttgtaa tttcccatgc tgcatcgagt tccattgctt caataaccca
    47101 accattattt gaacttgacg gatcatttgg aattccctgg gcaatgtttg tcaaatagtt
    47161 ggcagtaatc aatttagaga caaatccaag agatcctatt tcattgacaa tctgaacata
    47221 atcttgattt gaactaactc tgaaatcaat gaatttcaag gcatcatgat aggttattga
    47281 attcgtaaaa tgagaaccca gataatccat agcaattttt tcttgtccga ttactggtac
    47341 tatgaggaaa tggttttcaa taacctttaa agtatcgtca ccaacgatat tatagtattt
    47401 attgtttaat ttggttattg tccttccatt tacctggtaa ttggcctgac tgtaaaggtt
    47461 ttcaaatgag attagtgtaa tggataccac taatgctgca gataatttcg ataacgaaat
    47521 catgcggagg gggggatttt tttcatttta aataaattag tatgttatta ataatgtgaa
    47581 ttaagaagtt cacgtttaga tttcaatagc tttctggcct gatatcatca ataattctat
    47641 attcctaaaa gaaagcttta attttataac gctgtaaata tagtgaagaa atataaaaag
    47701 caaataaaaa aaacgcgttg ttcagattta ttgaaaaata ttcttgaatt acctgaaaat
    47761 tcactgatga aattaagaaa gccggtaagg caatcgtcaa atctgattct tccatagaat
    47821 ttttcaataa cggatactgc gttataatca tgcaccagac cctgaaggtt ttgaaaacct
    47881 tcagggtctt cggacatgat tgcgataatg atttctatct caatactctt cccaactcag
    47941 taccgccatt tcttcgggct tcagcttgca gatggcatag ataaaggcgc tggccagcct
    48001 ggcgttggtg atgagcggta tgttgaaatc cacggccgac cggcggatga ggtaatcgtt
    48061 gtttaactcc gtcttcgaaa ggtttttcgg aatgttgatc accaggtcga ttttgcggtt
    48121 gcgcaggtat tccagggtgt tgggagtggc ttcgtcatcg ggccagtaaa gggtgaccgc
    48181 ctcaacgccg ttttcgttca ggaacctggc agagccggga gtggcaaaga gatgatagcc
    48241 tttttcggcc aacatttgcg cgcttcggag cagttccacc ttcgaccttg catcgccggt
    48301 ggaaagcagg atgttctttt ccggaatcct gtatcctacc gagagcatgg ctttgagcaa
    48361 tgcttcatag taattctcgc cgatgcatcc cacttcaccg gtgctggcca tctctacgcc
    48421 cagcacggga tcggcggcat gcagccttga aaacgagaac tgcggcgcct tgataccgac
    48481 ataatcgagg tcgaaagccg atttatgcgg cttttcgacg gttttgccca gcatgatctg
    48541 cgtagccagc tcaatgaaat tgattttcag caccttcgaa acgaagggaa aactgcgcga
    48601 cgccctcagg ttgcactcga taacccggat gtcgttatcc tttgccagga actgcatatt
    48661 gaacggtccg ctgatgttga gggcgcgggc gatatcgcgc gaaacccgtt tgatacgccg
    48721 ggccgtttcg aaatacatct tctggggcgg gaatacaatg gtggcatcgc ccgagtgaac
    48781 gccggcaaat tcgatatgct ccgaaatggc ataggcgatg atctctccct tgtcggctac
    48841 ggcgtcgatt tcaatctcct tggcctgctg cacaaattcc gtcaccacca ccggaaattc
    48901 cctggaaacc ctggctgcca gggtgagaaa ataatccagt tcctggcggt tgctcaccac
    48961 attcatggca gcgcccgaca gcacatacga cggacgtatc agcaccgggt aacccacttc
    49021 gtcaacaaag tcgtaaatat gttccatttc gcttaactca ctccacctcg gctgatcgat
    49081 gtccaactga tcgagcaatg atgagaattt gtgccggttt tcggccttat cgatactttc
    49141 gggcgaagta cccaaaacag gaacgccctg ccggtgaagc cgcatcgcca ggttgttggc
    49201 aatctgtccg ccggtgctca caatcacccc tttgggttgt tccaactcga tgatatccat
    49261 cgtcctttcg aacgacagct catcgaaata cagccggtcg caggtatcgt aatcggtgct
    49321 taccgtttcg ggattgaagt tgatcatgat gccgcgataa ccttcatcct tagccgtttt
    49381 cagggcgttg accgaacacc agtcgaattc cacggaactg ccgatcctgt atgcccccga
    49441 tcccagcacg atcaccgatt tccggtcgtc ttcgggcacg atgtcgtggg catcagcatt
    49501 gtacgtaagg tagaggtagt tggtttgagc cgggtattcg gcggccagcg tatcgatttg
    49561 tttgatgtaa ggcacgatgc ccagcgattt ccggtaacgg cgcaattcca gcatttcggc
    49621 atgcagattt ccttcgtgtt ttgtaacaaa gcgggcaagc tgaaaatcgg aaaatccttt
    49681 ttgtttcgcc gttaataaca agcctgccgg gattttttca atgctgtcgc aggccttcag
    49741 ttgcatcatg acatcgaaaa ccgacctgag cttcaccagg aaccaggcat cgatctttgt
    49801 caggttatgg acttcctcaa ccgtcagccc tgcctcgaag gcgctggcga tggaatagat
    49861 acgcatatcg gttggttcgg ccagggcttt gcggatatcg gggaattcga tgtcgctgtt
    49921 gccgacgaaa ccgtgcgtcc cctgccctac catgcgcaac cctttctgga tggcctcctc
    49981 aaaagtccgt ccgatggcca tcacttcgcc tacgcttttc atgctggagc cgatatgctt
    50041 cgaaacgccg ataaatttat tcaggtccca gcgggggatc ttcacgacga cataatccag
    50101 ggcgggttcg aaaaaggcgg tggtggtgcg tgttacggca tttttcaact cgtgcaggcc
    50161 atagcccagg gccagtttgg ctgccacgaa tgccagcgga tatccggttg ccttggatgc
    50221 cagcgccgac gaccgcgaca ggcgcgcatt aacctcgatg acccggtagt cttcgctgcc
    50281 gggatcaagg gcatactgca cgttgcattc gccgacaatc cctacattgc gcacgatctc
    50341 aatcgaaagc tgacggagct tatggtattc gcggttggta agcgtctgcg acggggcaac
    50401 cacgatgctt tcaccggtat ggatgcccag cgggtcgaaa ttctccatgt tgcaaaccgt
    50461 gatgcagttg tcgtaacggt cgcggacaac ctcgtattcc acctctttcc agcctttgag
    50521 cgattcctct accaaaatct gggaggaata ggaaaatgcc ttcacggcta gtttcgtcag
    50581 ttcctcctcg ttattacaga agcccgaccc ctgtccgccc agggtgaaag cagccctgac
    50641 gatgatgggg aaaccgagtg tttcggaggc tttcagagca tcctctatcg tagtgacggc
    50701 aatgctcctc ggcgttttga tgttgatatg gcggaggttt tcggcgaata tttcacgatc
    50761 ttcggtatca ataatggatt gtatcggcgt gccaagcact tcgacgccgt atttttccag
    50821 tacgccggtt ttataaagtt cgatgccgca attcaacgcc gtttgtccgc caaacgagag
    50881 caggatgcct tcggggcgct cttttctgat gactttctca ataaagaaag gcgtcaccgg
    50941 caggaaatag atatgatcgg cgaccccttc actggtttgc acggtggcga tattcgggtt
    51001 gatgagcacg gtttcgatcc cttcttcacg caatgctttc agcgcctgcg atccgctgta
    51061 atcaaattcg cccgcctctc cgatcttgag ggcgcctgag ccaaggataa ggactttttt
    51121 cattttcagg tatttgtttc aaccacttag acactcagat cacatagagt cacttaggaa
    51181 aattcttttc cggaaggtca ttaaggcata gttctctaag gatcttaaaa ttttattttt
    51241 caggtaaaag ggcaaatttc tcagtcacca aaggaatcaa ctggctgaca atattttccg
    51301 aatgaaagtt aatcagcaaa ccctttggtt tattcgccaa tctcaggtag gataaaagtt
    51361 gagcctgata aagcgggatc aggttatcca cagccttcag ttcaacgatt atcaaatcct
    51421 cgaccagaaa atcgagtctg aatttaccgc ctaaatcttt ccccttataa ctgacgggaa
    51481 cccaaacctg cgattcaaat ttcagattcc agtttcgcat ttcatccttc atacagttgt
    51541 gatatactga ctccagtaat cctggtccta aagttttatg cacctcaata gcacaaccga
    51601 caattgtgta tgaaatttca ttgaccagtt ttttagttat aatagccatt ccggatattt
    51661 acgctaatag acttctatta ctcttagtga cactttgcga taatttcaga ctcaacctaa
    51721 tttattatct tttacgatgc caatttttag tctataaaat ctgttcatcc atcctatact
    51781 acacacttaa aacgtgctgt tcctttcagg tcctcaacaa tctcagtgta tctgtgtgcc
    51841 ctaagtgtct aagtggttct atttgttgtt gatcataccc aggaactcat caaaaagata
    51901 ttctgtatcg gtggggccgc tggaagcttc ggggtggaac tggacggaaa agatcttttt
    51961 ctttttgttt cgcaaccctt cattggtgcc atcgttgagg ttgatgaaga aaggttccca
    52021 ttcatcggaa agcgtgttct gatcaatggc aaagccgtgg ttctgggatg tgatgaatgc
    52081 ccggttgctg ccgcaaagca acaccggctg gttatggctc cggtggccgt attttagctt
    52141 gtaggtttcg gctcccgacg ccagggccag caactggttc ccaagacaga taccaaaaat
    52201 tggcgtatcc tgttttatcg acgctgacaa atgccggatg gtggcttcac acatcctggg
    52261 attgccgggg ccgttgctga tgaaaagccc gtcatattcc acgcccgaat aatcgtaatc
    52321 ccagggaacg cggatgaccg tcgtgccgcg tctgacgaag tgtctgatga tgttgtattt
    52381 cacgccgcaa tcaatcagca ggatcctggt ccggccttcg ccgtatgtaa tcacttccct
    52441 gcagcttacg gtatcgacaa ggttctgttg gttgggatcg atgaaatcaa tgtcatcatc
    52501 tccgaaaacg atcttgccca acatggcgcc tttttcccgg atgatcttgg tcagcgcacg
    52561 ggtatcgatg ccatacagcc ccggaatttt atattctttc agccagttgc cgaggctttg
    52621 ggcggcgctc cagtggctgt actgttccga gtaatcggaa acgatgagcg ctgttacctg
    52681 tatccgttcc gactcgaaga aatcgggcag cccgttggtc agtctgatgc ccggaacacc
    52741 gtaattgccg atcagcggat atgtagcaac caggatttgc ccggcgtaag aagggtcggt
    52801 caggctttcg gggtaaccgg tcatcgaggt gttgaaaacc acttcgccgg ccacagaccg
    52861 ttcatctccg aaggattttc ccgtaaaaac ggtgccgtct tccagcatta atcgtgctga
    52921 cataggatgg ttcatcatat actatttaaa gataattcat aaatcgcaac ttatcacagg
    52981 gttacaggca tttttacaca aattgggatc gccgtcgcgg acagcaatcc caaaaaatga
    53041 attcaaaaac aaactgatct atacggtgca aaaattgcgc ggtaacagcc tgccttcccg
    53101 gctaattatt gccgataatc tgcaataatt catcgagttt aggcgtgagt atgatctcgg
    53161 tacggcggtt cttcgccttg ctggctgccg aggtgttggg ctcaacaggc agatattcgc
    53221 tccttcccga agctatcagg cgcttcggtt ctatattgct gttcgcggta agaattttca
    53281 cgatggctgt cgccctcatg acgctaagat cccagttgtc tttcacctga ccggtaccat
    53341 tgtaaggaac attatcggta tggccctcga tcatcacgct gatatcggga ttatcttcaa
    53401 gaacccctgc caggtttttc aaagccgcca ccccgtcggg gctaacatcc catttgccgg
    53461 aagcaaacaa caatttttcg tccatcgaaa cgtacacctt tccgtttttg gtatatacgg
    53521 taagcccttt cccttcgaaa ccgagcaagg catcagctac cgcttttcgc atggctttgg
    53581 tgatggaatc gtttcggttc agcgccgact gtaattcccg gaggctgagg cttttggcgt
    53641 cgagttcggc ctgaagctgt ttgaggttgt cctcgcggac gataaggtta tcctgggcat
    53701 ttttcaaatc ggccataata cgttcggttt cctcaaggtt accttcgttc tgcgctttga
    53761 gcatggaaaa tgactggtcc aggtcgctgt agtctccctg caaaacacgg taggcagaac
    53821 caagacgggc agtatcggat ttcaactccg tattttcatt tttcagtttc ctgccttgtg
    53881 ccgtcagttc attcacttct gtcgtcagct cgtcaacctg tgctgtgagt tttgtattct
    53941 catccttgca acgggttgcc agcgaattat actttttggt tgaaacacag gaagtgaatc
    54001 ccgccaaaag gatcattccg gtcattatca tgaggctttt tctgtacata ttgtcaatat
    54061 tggtatcgga gtaaatattc agtcatttat ggttttaata acagatactg tttttattta
    54121 ttcatccatt tcaagcagaa cgggacaatg gtccgaatgg ttggccattg acaggatggc
    54181 ggcccgtttc agccgctttt cgagcgatcg ggttaccatg ttgtaatcga tacgccaacc
    54241 caggttccgt ggccgcgccc ccgcccggta actccaccag gtataatggt gcggctgggt
    54301 attaaagtaa cggaatgtgt caataaaacc atcttccagg aaaccggtca tccattcgcg
    54361 ttcctcgggg agaaagccgc tgcttgcagc atttcgtacc ggatcatgga tatcaatggg
    54421 tttgtggcaa atgttgaaat cgcctgaaat cacaaggttc ggatgcgttt cccgcaaacg
    54481 ggccatatac tccctgaaat acgacagcca ccgcatttta aaatcctgcc tctccgggcc
    54541 catcgtgccc gacggatgat aaacagagat caccgaaata tcccccatat caatctgcat
    54601 gaaccgccct tccccgtcat attcccgttt cccgatacca taacagatat tgccgggctt
    54661 gattttagtc aggatgccta cgccgctgta tcctttcttt tgagcggggt gccaataatg
    54721 atgatagccc atcttctcaa tttccccggc agggatgtcg gcttccaggg ctttgatttc
    54781 ctgcagacag ataacatcgg gtgatgtatc gtgaatccat cccaggaggc ctttatggat
    54841 ggccgcacga atgccgttca cgttgtaggt tacaatcttc atcgcccttt tatcagcaac
    54901 ggcaaatata atgttaaaaa gattgcttct ccgccatgct gaacagcaag ttatgaaaag
    54961 aaatcaacaa tatgatggct aagagagagg attgaaaacc cgtcacaaaa aagcgccgga
    55021 ttccaggctt ttcagaagct ttcttattca tcaaaaattt cacaggttcc cctgcatatt
    55081 cctccgcagt tgtcgaggca gggctcggta tgtatcatca ccacggcgtt gggaagttcc
    55141 tttttgatac gcagggttat atcatcgcag acatcatgcg ccgctttcac gctcatatcc
    55201 ttgttcacca cgaggtgaaa ttcgatgaaa cggtcggctc ccgctttgcg tgtcttcagt
    55261 ttatgaaagc tgagcacgat ggggttcatc gactcaatga tacttcggat tagtttttcc
    55321 tcttcgtggg gcaggctgga atcgagcaag gccatgatac tttcggaggt aagttcccag
    55381 gctgctttca ggatgagggc ggctacgccg agggcgataa ccgggtcgac ccagtgaagg
    55441 ttcagttcgg ggaagaaata cctgcccagg atatacagca gcaatccgag agaaacccct
    55501 gccgaggtcc atacatccgt ccgcaggtgc cagccgtcgg ccatgagggc agcagaatcg
    55561 gtggcgcgac ctactttaaa caggcggttc gatatgatga agttggcagt tgccgatacc
    55621 gccatgacaa tcacgcccca ttgtaccgcc tcaacaggcg cgggatacat gagtttcttc
    55681 acggcttcga cgatgatcca tacagccgca acgaagatga ggaaaccttc aatcgccgct
    55741 gacaggtttt caaatttacc atgaccgaaa gtgtgttttt cgtcagcagg cttgcccgat
    55801 gctttaacgg caaagagggc aatgagagca gctaccaggt ccaccgccga atggatggct
    55861 tccgaaatca ccgaaacggc gccgatcatc aggccaacag ccagtttaaa gaccacgaga
    55921 agcgaatttg ataatacaga aagcctggct acctgtcgtt tgcgttgttg cgatttcatg
    55981 agcgatacta ttcgttaatg agctatgagc ctgataaaat gctgtacctg aatctttttg
    56041 gtttaccggt tatgcaaaat tacgcaaaac ccgctttctg caaaaacctt taccggattc
    56101 aaaaacgccg cacgtaacga atattttgaa agccaatggt ttttattgaa gtaatcagga
    56161 gactattttt tacttttgaa gcatcatgta taacaaccgg ctttatcaga aaatacacag
    56221 tttccggctc aggcatattt caccgcggac gttcctggtg atactgagtg tgataaccgg
    56281 cgcttttggc ggcctggcgg ccgttattct taaaaatgcg gttcattatc ttcatcttct
    56341 gctcacatcc ggctttaaca tcaaagaatt caaccttctt tacctcgcat atcctttctt
    56401 cgggattgtg atcacctacc tgtttgtcaa atattgcgtc aaggataata tatcgcacgg
    56461 catcagcaag atattacgtt ctatttcgaa ggaacaatcc attgtgaagc cgcataacat
    56521 gttcacgtcg attatcgcca gcacctttac ggtaggtttt ggcggatcgg tcgggcttga
    56581 agcgcccatc gtgctcaccg gatcggcgct ggggtcgaac ctggggcgta ttatgaggct
    56641 tgattacaag accacgacat tgctggttgg ttgcggcgca gcaggcgcca ttgcgggtat
    56701 ctttaacgca cccatcgccg cggtgctttt cagcctcgaa gtgctgatgc tcgatcttac
    56761 catggcgagc cttatccctt tactgctttc ggccgctacc ggcaccaccc tgtcatattt
    56821 tcttatgggc cgcgggttcc tgttctcgtt tcatgtcgcc gatccttata aactgaataa
    56881 cctgccgttt tatatcctgc ttggcgtttt taccgggatc acctcggttt attttaccaa
    56941 agccaatttc tcgctcgaat cgaggtttaa agccattggc aacagtacct tgcggacggt
    57001 tacaggcgga ctgatcacca gcctgctgat attcctcttt ccgcctctct tcggagaagg
    57061 atatacctca ctgaatgctt tgcttgaagg ccacgggaac agcattgtgg aaggcagcct
    57121 gttttatggc attcagggaa acacgctgct tttcgccgga ttcctgttgc tgctcattat
    57181 ctttaaagtc attgctatga cggccaccaa cggtggcggc ggcgtcggag gtatttttgc
    57241 cccttccctt tttgtcggag gcgttgccgg ttacctgatg gccctggtta tcaacatctt
    57301 ccttccgggc agactgccgg tcgaaaattt tacactcgcc ggcatggcgg gcgtcatgtc
    57361 gggggtgatg catgctcccc ttaccgccat cttcctgatc gccgaaataa ccggcggtta
    57421 ccagtttttt atacccctga ttttcaccgc tatcgtttcg tacctcacca tcatgtactt
    57481 cgagccgcat agcatctata ccaagcggct tgccgaactc ggcgaactga taacgcacca
    57541 caaggataag gctacactct cacgcatgac catcgaaaac ctgatcgaaa caaacttcct
    57601 gaaagtaagc cccgaagcca cccttggcga actggtgaaa attgtttcac agtcgcagcg
    57661 taacatcttt ccggtggtag atggtgataa caatttcatg ggtgtgatct ttatcaacga
    57721 tatccgccat ataattttca accacgatct ctacgacaac gtatatgtaa gcaacctgat
    57781 gttcatgccc gatacttttg tgaaccttga cgccacgatg gaagacctgg cccagaagtt
    57841 tcacgatacg gcgcattaca atattccggt actcgacaaa gggaaatatg tcggttttgt
    57901 atcgagggcc aacgtgtttt cggcttaccg caaactggta aaagagtttt cggaagagtg
    57961 acgttccctt aaaatggcga tggcctcttc cacggtcgca accggcgcga ggcaagcctg
    58021 gccgtagcaa atatagatca gcgtcttatt tttgttgaac cgttccctga aataagggag
    58081 ttccgacggc tttgaacttc cggcaatgaa aacgcccggc aggaagtatt tcccgaattc
    58141 agccatatat tctccggcct tttccccgca tgctgcaagg acacaggccg gctgcgacct
    58201 ttccatcgcc agggtgcacc agttggcata tcccagcgga tattcctttg ccccggcaat
    58261 tgtccgggcc atcatccggt cagcggtatc gcaccaggag ctgtaatcca taaaggttcc
    58321 cagagtaaaa agcagccctg ccaggatgga attggaggaa ggaatcgcgc cgtcattggt
    58381 ttcaatggtt ccggcaaata aacgggattt ttcgccggag aagaaaaaca gtccggaagc
    58441 cggatcctga aagtgttcca ggataaaagc gataagcccg gaacattccc cgagccaata
    58501 ttcatccgaa gttgcctggt agagcctgag cagggcgcca gccataaaag cgtgatcgtc
    58561 gaggaagccc tcaatacggg cccggctgtt tttccaggaa cggaacaaac caccttccgg
    58621 gtttttcata ttattgagga tgaaggcagc cgcctggcag gctgtttcga tatattccct
    58681 gtttccgaaa gccatgccgg catcagcata agctttgatc atcatggcat tccatgccgt
    58741 cagcactttg tcgtcggtgg ccggacgggg acgccggttt ctgtatttat gaaggtccct
    58801 ctttaccctg gcaacaagcg ccgaaagctc atcctgcgaa aggaagtgcc gttgcgcaaa
    58861 gagggcatca ttatgaggcc tgagcaggat attcctttca tgttcccaca gtccttcctt
    58921 tcccactcca aaatattcag ccatcaggct gctgtagggc ccgagcgctt cttcaaattc
    58981 gggataagtc caggtataaa aaaggccttc ttccccttgg ctgtcggcgt ccagggagga
    59041 acagaacacc ccttcgggcg aagtcagttc gcggctgaca aacgccagcg tctcatcggc
    59101 caccttcctg aacagttcct ctccggatat tttaaaagca tcacagtaaa ggctgacaag
    59161 gagggcatta tcatacagca tcttctcgaa atgcggcact ttccaggcgg catccgtcga
    59221 ataacgggca aaaccgcccc cggcctggtc gtaaattcca ccggcagcca tgctgtgcaa
    59281 cgtttttaat gccatttgca acgcatcacg gttgccggta agcgcatgaa aatgcagcag
    59341 catcccggca accgaaggca tgggaaattt gggagcatgg ccaaagccgc cgttcaggga
    59401 atcgaaccgg ctctgcaggt ccgttaaaat agtctcaaaa tcggtttttg taaatcccgg
    59461 gtgctgctgt tccggcgcga agagcgatga cccggccatt ccccggacaa tctcagacgc
    59521 ccgggcttcc gcatcggcta acctgattct gaagaattca gcaatccgga tcaggatttc
    59581 tttccatggc ccgggcccga aatacgtccc tccccagaac ggcctgccgt cgggaagggc
    59641 gaaacagttg agcggccagc ctccctggcc ctgtatgagc tgcacggcat tcatgtaaac
    59701 atggtcgacg tcgggtcgtt cctcacggtc aacttttaca caaacgaagt tttcgttcat
    59761 gatgccggcg atcccggcat cctcgaacga ctcacgttcc atcatatggc accagtggca
    59821 ggccgaatac ccgatgctga tcagtaccgg gacattcctt cgcttagctt cagcaaaagc
    59881 ttcatcaccg tatgggaacc agtctacggg attatgcgcg tgctgcaaca ggtaagggct
    59941 ggtttcacgg atgaggcggt taggcatggg ataggttgtt attggctgtt ttgagttctg
    60001 tgttttgagt tctgtgtttt ggcttttagc ctttagccct gccattctcc cacagccttg
    60061 cgcaaggcag gtaaactttg cctcttgacc ttt
//
LOCUS       DAOPYV010000007        59267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_383_length_59267_cov_11.412011, whole genome shotgun sequence.
ACCESSION   DAOPYV010000007 DAOPYV010000000
VERSION     DAOPYV010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 59267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59267
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_383_length_59267_cov_11.412011"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..959
                     /locus_tag="PKI35_01560"
     CDS             297..959
                     /locus_tag="PKI35_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNX43092.1"
                     /translation="MQLVSNYENLYKALGGKVRMVAVSKTKPVEMIRELYDAGQRDFG
                     ENRVQELTAKSAVLPADITWHFIGHLQTNKVRFIVPFVGYIHSVDSLKLLSEINREAA
                     KINRVINCLLQFHIATEETKFGLSFEEAAALLNNPDTARMQNVRICGIMGMATFTDNK
                     QLIRNEFRQLKSHFDKLKTSHFNDDPAFREISMGMSNDYEIAIEEGSTMVRIGSLIFG
                     ER"
     gene            complement(1056..2105)
                     /locus_tag="PKI35_01565"
     CDS             complement(1056..2105)
                     /locus_tag="PKI35_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNX43093.1"
                     /translation="MPSFTLLIIIIVLAFSFDFINGFHDAANSIATIVSTKVLTPFQA
                     VIWAAFFNFLAYAIFELNIADTIAKVVDTNAIDLYVILAGIIAAIIWNLLTWYLGIPS
                     SSSHTLVGGFAGAAVAHAGWGIVHTGVIVKIAAFIFLAPFLGMFFSYVISVTLLNILR
                     KANPYKLEKVFKKVQLFTSALFSLGHGGNDAQKVMGILAATLIVYFHGADPSQIPHWA
                     HITLVNGKIHAVPHWIVLGCYTAIGCGTLLGGWRIVKTMGSKITKLTPFEGVAAESAG
                     ALLLFGTEAFGIPVSTTHTITGAIMGVGMTKRLSAVRWGVTINLLWAWILTIPVSMII
                     SAAVYYLFLLMNWAI"
     gene            complement(2169..2816)
                     /locus_tag="PKI35_01570"
     CDS             complement(2169..2816)
                     /locus_tag="PKI35_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNX43094.1"
                     /translation="MKLDRIFGFLVPKDKKFFPLFIQAGENLVVASELLVKLIRENDM
                     QKREEFIVAIKKAEKNGDNITRDLMDELNSTFITPFDREDIHELASTLDSVVDFIHST
                     SKRIHFYKLHTFPPEFVTIADLIHDANKEILNVIRDMKDVAGLARHKDSCIRISEIEN
                     EVDDIYQAFLADLFENETNAIDLIKKRDILASLEKAIDKCDDVGDVFSGIMIKMS"
     gene            complement(2980..4518)
                     /locus_tag="PKI35_01575"
     CDS             complement(2980..4518)
                     /locus_tag="PKI35_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNX43095.1"
                     /translation="MLTSEGNMLIAKSASLKELFISPKMANRHGLIAGATGTGKTVTL
                     QVLAEAFSLKGVPVFMADIKGDLSGISNTGVDNPKILERVQALQLQGYMHRGFPVALW
                     DLYGEQGHPLRTTISEMGPLLLSRLMNLNDIQSSVLTIIFKIADDNNLLLLDMKDLKK
                     MCEYVGNNRDQFITAYGNISVASIGAIQRELITLEQQGADKFFGEPAIDIFDFIQTDS
                     NGLGQINILAADKLMMSPRAYSTLLLYLLSELYERLPEAGDLDKPKLVFFFDEAHLLF
                     NEAPRVLLDKIEQVVRLIRSKGVGIYFCTQNPIDVPDVVLGQLGNKVQHALRAFTPRD
                     QKAVATAAETFRPNPEVNVEQVITELGVGEALISFLDEKGIPLPVERAKVLPPQAQIG
                     PVSPEERSRIMRSSLIAGVYEKEVDRESAFEILQARINKEQEQIRMEEEQERLEKERI
                     QAEKERAKAEKEAAAEERRNHSKGDIFTDLTKTVGRSVTSTIGREIGRTLIRGLLGGL
                     TGRR"
     gene            complement(4755..5333)
                     /locus_tag="PKI35_01580"
     CDS             complement(4755..5333)
                     /locus_tag="PKI35_01580"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNX43096.1"
                     /translation="MKDPEFQEDIENALKTLQSGGVILYPTDTIWGLGCDATNHNAVE
                     KIYRIKQRIESKSLIILIDSFEQLNNYVDKIPDVAYDLLKCIENPVTVIYSNAKNLAS
                     NVIAGDGTIGIRIVKEEFCINLISRFGKPIVSTSANISGEESPVIFSHISKDIISNVD
                     YIVKYRQETFSQSKPSTIIRLMETGEYIIIRQ"
     gene            complement(5330..6325)
                     /locus_tag="PKI35_01585"
     CDS             complement(5330..6325)
                     /locus_tag="PKI35_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNX43097.1"
                     /translation="MPRFLIIQTASIGDVILATALLEKLHHHFPDATLDFMVKKGNEA
                     LFGEHPFINELLVWDKSGNKYQNLWKLLKQVREIRYDRIINLQRFASTGLFTVLSKAS
                     YTAGFNKNPFSRWFTHQAEHHIPAKDGAHEVARNQALIAPFTDNLPARPRLYPTASDI
                     KCVEAYSSDRYICVAPASLWFTKQYPASRWILFIKELPATIGVMLLGSGDDKDLCDNI
                     INESGKDRCYNLAGKLTLLQSAALMKGALMNYVNDSSPMHLASAMNANITAIFCSTVP
                     QFGFGPLSDNAFIVETPLSLACRPCGLHGHRECPENHFNCATSIDTRQLLSNLAL"
     gene            complement(6366..7178)
                     /locus_tag="PKI35_01590"
     CDS             complement(6366..7178)
                     /locus_tag="PKI35_01590"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNX43098.1"
                     /translation="MNELKTLIDNAWVERSLLKNAETQQAVYSVIEMLDKGSLRVAYP
                     ELDTWKVNDWVKKAVILYFPLRQMETIEAGPLEFHDKIPLKHGYESLGIRVVPHAVAR
                     YGAYISPGVIMMPSYVNIGAWIGPGTMVDTWATVGSCAQIGASVHLSGGVGIGGVLEP
                     VQAAPVIIEDHCFIGSRCIIVEGARIGSEAVLGANVVITGSTKIIDVSGSEPVEYKGY
                     VPPRSVVIPGSIEKEFPAGRYNVTCALIIGKRKPSTDLKTSLNEALREYNIA"
     gene            7319..7738
                     /gene="ruvX"
                     /locus_tag="PKI35_01595"
     CDS             7319..7738
                     /gene="ruvX"
                     /locus_tag="PKI35_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNX43099.1"
                     /translation="MGRVIAIDYGQKRTGLAATDELQMIASPLCTVPTVKVMDFLRDY
                     IQREKVDVLVVGEAVQMNGSPSSSAEFIEPFVRLLSRTFPGIPVERVDERFTSKMASR
                     TILHSGAKKKERQDKGLIDRVSAAIILQSYLERKGMD"
     gene            7792..8358
                     /gene="def"
                     /locus_tag="PKI35_01600"
     CDS             7792..8358
                     /gene="def"
                     /locus_tag="PKI35_01600"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNX43100.1"
                     /translation="MILPIVAYGHPNLRKISLDITPEYEGLNELIANMYETMYYSSGV
                     GLAAPQVNRQIRIFVIDATPYGKEHPEASTFKKAFINPRIVGEKGEEWAFNEGCLSIP
                     DIHEDVLRPADVHIIYRDEEFVEHDEWYSGVIARVIQHEYDHLEGKLFVDKIHPLRKM
                     MLKRKLNDISRGNIDVSYKMIFPQKKKY"
     gene            8389..8928
                     /locus_tag="PKI35_01605"
     CDS             8389..8928
                     /locus_tag="PKI35_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX43101.1"
                     /translation="MKKIISGTFAILLILLFSSCGSSPEKMSAKIAKLEKEVKEQSPA
                     DTVKINELVEAYSKYASKYPKDTLAPEYLFRAGGMCLSFNKPSKAISLFQQVEKDYPS
                     FSRVGECLFMQGFIYENALGDFSKAGSIYREFIAKYPGHPLADDAEMSLKYLGRPAED
                     IVRDFEKKEADSAAVVQEK"
     gene            complement(8977..9765)
                     /gene="cdaA"
                     /locus_tag="PKI35_01610"
     CDS             complement(8977..9765)
                     /gene="cdaA"
                     /locus_tag="PKI35_01610"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNX43102.1"
                     /translation="MLSFLSVRVLDIVDILLVALLLYWLYQLLKGTAAVNIFLGILAI
                     FALWRVVKALGMDLLSDLLGAFISVGFIAFIVVFQPEIRKFLLAIGSPGFFKKTSSRF
                     LFWKIKLEDENLSDIDPVVQACQRMAMTKTGALIIVSRKNDLTEFTETGVAIDATITD
                     PLIESIFYKNNPLHDGAMVISLNRIKAVRCILPVSSSSDLPNDLGLRHRAALGVSERT
                     DAISIIVSEQTGNISYCRDGQLSVDIKAAGLRNMLLNDFSPAVK"
     gene            complement(9807..10673)
                     /gene="folP"
                     /locus_tag="PKI35_01615"
     CDS             complement(9807..10673)
                     /gene="folP"
                     /locus_tag="PKI35_01615"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNX43103.1"
                     /translation="MKPKNTVFPHSRTLKCGDKLLNLSEPVVMGILNVTPDSFYDGGK
                     YKNLDACLAHADQMIAEGAKIIDIGAVSTRPGAPEVTESEELQRLLPVLNELVKRYSR
                     IIFSIDTFRSSVARATAACGANMINDISGGDLDENMFAVMAGLPASYVLMHMQGTPAT
                     MQVNPVYRDVAGEVFDSLQAKLQKLQEMGVGSVVIDPGFGFGKTIQHNYELLRNLHRF
                     AELRCPVLVGVSRKSMIYKLLNISPNEALNGTSILNTLALLKGADILRVHDVKEAVET
                     ISIVNEYLKAGK"
     gene            10697..11269
                     /locus_tag="PKI35_01620"
     CDS             10697..11269
                     /locus_tag="PKI35_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HNX43104.1"
                     /translation="MIHHKDIDSMAARTSEQFDAVIDSCRRIFLDKMHDYGTAWRILR
                     PSSLTDQIYIKASRIRSLQEKGHSRVGEGICPEFMGIVNYSIMALIQLELGSSEEARM
                     ESEKVETLYNTNAAKAKSLMTDKNHDYGEAWRQMRVSSLADIILMKLLRIKQIEDNNG
                     VTLISEGLDANYLDIINYSVFALIQLNDQK"
     gene            11300..12421
                     /locus_tag="PKI35_01625"
     CDS             11300..12421
                     /locus_tag="PKI35_01625"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNX43105.1"
                     /translation="MKILANISRLLTACVFLFSGFVKGVDPLGFSYKIEDYFIAYHMD
                     WAIPLALTLAIMACALELSIGVMLLLKLRMKPASWLLLLMMSFFTVLTFFDAVYNPVP
                     DCGCFGDAIILTNWQTFYKNIVLIVLAIIVFSYRKKFSNGFSPSAQWMITGIVAALFI
                     SFSVYCYRHLPVIDFTEWKKGHKLYAENPVPVKYFVTYRNNATGEQKEFLSPNYPFDD
                     STWMAQWVFVSQRVEDPNKYYGKSLVITDTLGNILTETVTRNPEFQLIVNAYDLSSAN
                     SQAFSAIDKFCLKAAGDGVSSVALVASEPADIVNFVHRNKLSIAFYQSDDIVLKTMVR
                     SNPGIMLLRNGIIIDKWNWRDLPDYEKFKAEFLTQKEPS"
     gene            12437..13519
                     /locus_tag="PKI35_01630"
     CDS             12437..13519
                     /locus_tag="PKI35_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX43106.1"
                     /translation="MLQYTLKRLLYGFLVLLGVITLIFVLFNTLPGDPARMILGQRAD
                     LASVQAINRDLGRDKPLTVQYFNFLNDISPISVHNCANSGSYWFLDPEKYASAIPLFN
                     IGNAHLLVLKQPYLRRSYQSKRPVSEILADAFVPTIILATVAMLLATLAGLLIGIFSA
                     LHKDTWFDKTALLLSVLGMSLPSFFAAILIAWIFAFVLSDITGLSMFGSLYSVDDFGN
                     GEYISLKNLILPVFTLAIRPLAIITELTRSSMLDVLGQDYIRTARAKGLSERKIIVRH
                     ALKNAMNPVLTAVSGWFASLIAGAVFIEYVFDWKGIGVVIVDALEKFDFPVVMGAVLC
                     ISVFLVIINILVDLAYGLLDPRIKYS"
     gene            13572..14336
                     /gene="tpiA"
                     /locus_tag="PKI35_01635"
     CDS             13572..14336
                     /gene="tpiA"
                     /locus_tag="PKI35_01635"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNX43107.1"
                     /translation="MRNKIVAGNWKMNTTFQEAEDLLYEITDLLNERGKNDTMIIICP
                     PSPYLEMASDIGVENDFFVGAQNINDHEKGAYTGEISAPMISSMGITHVILGHSERRT
                     YYGETDAVIKAKIDLALANDLVPIYCCGEVLAERQKSNHFEVVRQQISEALFHLDADV
                     ISTVIIAYEPVWAIGTGVTATPAQAQEMHAFIRGLIGSKFGDDVADKINILYGGSCNA
                     GNAAELFSQPDVDGGLIGGASLKAGDFVTIVNSFSE"
     gene            14372..15205
                     /gene="prmA"
                     /locus_tag="PKI35_01640"
     CDS             14372..15205
                     /gene="prmA"
                     /locus_tag="PKI35_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNX43108.1"
                     /translation="MDYYEFRFRLKPVDDAGDMLVAMLGDIGFESFIEEDNVLKAYIP
                     VAGYTEECISSLSGDQIIRWFASYETELIRDRDWNALWESGYSPVVIDDKLIIRAPFH
                     PKRDEIPLEIIIEPKMSFGTAHHETTRLMLRYILELDMENKSLLDMGCGTAALAILAR
                     KSGANPVTAIDNDEWAYNNSIENIANNNITDIGVYLGDAGLLEKKRYDIILANINRNI
                     LLRDIPAYSACLDPGGLLVMSGFYTGDLDSVSQRARESGLELLSDREENKWVAACFIK
                     R"
     gene            15223..19719
                     /locus_tag="PKI35_01645"
     CDS             15223..19719
                     /locus_tag="PKI35_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43109.1"
                     /translation="MNRKRLCCVFIFLLTAGFVDAQEEPAKTFSADTSKFVGDLTAFF
                     ATVGEGDAKEARDVLSDFTYHWTSNLLTPAQKKQVQSVCNAMLEQKFKVAPHFIEYLK
                     SISLMVRRNIPAKAFDTHYLSVNYCLEGKNSAQNLAAYLTQTNLLLADYAFRLNNTEA
                     WRMRDARFEFDFDTVPVFRFSSATLSCIVRGDSTNIYDTRGLYFPITQQWLGNGGKVY
                     WDRTGLGRDSVFAELSTYRINLRTTSYSADTVSFVHRGYFAYALAGRFEERVITDVAP
                     ERASYPRFTAYAGDLYIHNLFRNIDYEGGFAMEGARVLGGGVNKSTAIIHVQRNNLPF
                     IELRSGEFVIRPDRFVANRANATIFIGNDSIYHPGLLVRYIKSNDEFVFTRMEEGLSQ
                     SPFFNSYHLVDMYPEAIYWKMDDDKLSLEAIRGPRSKGDALFESADFFSQYRYDRMQG
                     MDEVNPANLVSTYIRKHSTDRFYADEYADYIRMPLEQVRVQLIRLANAGFIIYDMDND
                     FVTVKPRLNEFLAARGGFKDYDILQFYSTVEKGSNAILDLKSLDLTLYGVRQVMLSDS
                     QYVYVVPRDGKIVMKKNRDFTFTGRVHAGFFEFYSNECSFDYDHFRMNIPKIDSMAMY
                     VYAWHPDLNGYQPLIRVKNVISDMSGDLQIDDPGNKSGKKSLKNFPVFESKDISYVYF
                     DKKSTVQGVYKRDKFYYSVDPYIMDSLNHLPTANLKFEGHLNTGDMFPVIDEPLRVQE
                     DYSLGFIKKLPAGGLPVYAGSGVYSDTLKLSNNGLRGSGKLTYLASTSESRDFLFTPD
                     STSATLKNFNLKETVASARYPQVAIRESRVKWLPGKDVMTVSNLPGDSISLFNNHALL
                     SGSLALTPEGLRGRGKMTFEDAQVNSNSYDFINNAFTSDTSDFALYTPDHKSQVLSVH
                     VFRTDIDFDKRLGHFTASGKGSRMEFPVNKYVSLVDGFDWMMDKKVLQLLTKTTFTED
                     AYHRMNRDQLLNIPSGDDRFISSDYAQDSLSFFAMRANYDLTSNVLQVLDARIIKVAD
                     AAIFPVNGEVTIGRDGVMNELKGTTILANTDTKYHELYNADVVISSKNHYGGKGLYDY
                     VPADGDLQTLQMSTINVNSERQTYATATVTETDNFKLSNHFGFEGDIELNAPDKMLSF
                     QGTFNLVHECEALGHEWIKINAKIDPANIMIPVSNDMENRGNGNLRTSILYSSTKQGL
                     LPAFFEKPQNINDQDIIAATGYLIFDTLRREYRIAPKEKLEDVSKPGNMLAFNTNQCI
                     LEGSGKINLVGDMGRLTMKSAGKGAYFMIPDSATFMVTTAFDFFFSEEALKVFSTDLK
                     IADLKGMDVAATSYSFPLYEFLDTAMAQKQMAELTSFGQFRKFPEELNHTLMLSEMKL
                     AWNPELRSFVGKGPVGIMSLGKEMVNKYVESYLEIGKRRTGDMFTFYFELEDRHWYYF
                     SYANGIMQAISSNKDFNDKLVGLDEKDRTIKSGDGQRSYQFIVSNNEKKGSFLRKMKQ
                     AFGE"
     gene            19975..20613
                     /gene="plsY"
                     /locus_tag="PKI35_01650"
     CDS             19975..20613
                     /gene="plsY"
                     /locus_tag="PKI35_01650"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNX43110.1"
                     /translation="MISYFPIVSGAVIAYLMGSFSSSVWIGRAFFSVDVRDEGSHNAG
                     ATNTIRVLGVKAGIIVLLLDALKGWGAVALSALFAGSFFSGERLVYYKILAGLMAVLG
                     HVFPVYTGFRGGKGVATLVGVVVALFPKVFWIVLVWFFTVFLATGYVSLASVTSAILL
                     PFISFFIFHEKSMAIIVLSILIAVFIPVTHHKNIRRLIRGEESKLKLWKKKP"
     gene            20858..21985
                     /gene="dnaN"
                     /locus_tag="PKI35_01655"
     CDS             20858..21985
                     /gene="dnaN"
                     /locus_tag="PKI35_01655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNX43111.1"
                     /translation="MKFIINSQTLLRKLQSLSGVLNSSNTLPILDDFMFELTDTALNI
                     TATDLETTMVISLEPSMLEEPGSITIPAKLLLDFLKYLPDVPLTFSIDLALQAVEIVS
                     SDGKYHLTGHNAEDYPKLPELTETARIEMPSDLLAAGINKTVFATGNDELRPAMGGVF
                     FELTPDYLTFVATDAHKLVRYRRTDAKAEDSASFIVPRKPLNVLKNLLATDIAQVTVE
                     YNVTNAAFEFQNVRLYCRLIEGKYPAYEAVIPRDNQNHLTVDRQVFINAVKRLSPFAN
                     QSTHQIRLRISGQELVMTAEDIDYSNEGSERLNCTYDGEDMEIGFNSKFVLDMVTNLE
                     SDEICFNLAGPTRAGIILPVNNENKDEDILMLVMPVMLAQG"
     gene            complement(22099..23988)
                     /locus_tag="PKI35_01660"
     CDS             complement(22099..23988)
                     /locus_tag="PKI35_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNX43112.1"
                     /translation="MNILQVEHLSKSFGEKVLFHDISLGISKGQKIALIARNGTGKTT
                     LMNIIAGQDAADEGRVTIRRDTRVAYLRQNPQYEASKSVLDILFTGDNEFMNAIHDYE
                     EALERFRHEHSEAHQRALDAGMARMDALQAWDYESKVREILGRFGISDLSKPAGDHSG
                     GQIKKIALAGALIGTPDLLLMDEPTNHLDFEMIEWLEEYLSRESLTLFLITHDRYFLD
                     NVCDSIVELEQDSLWQYKGNYSYYLEKKALRQSAEEQGLEKNTNLYRTELEWIRRMPK
                     ARGTKSRARIEAFDELKEKVSRKPEEKTIKFNVNANPLGGKIIEVNNICKKFGDNTII
                     DDFSYIFKRGEKIGVVGLNGAGKSTLLNIISGNMRPDKGSVVTGQTVVTAYYTQEGLQ
                     PVEDKRVIDIVKDVAEEIVMKKGSLSAAQFLYQFNFSYAVQHAYFSALSGGEKRRLYL
                     LLQLMRNPNFLILDEPTNDLDIFTLQVLEEFLGDFPGCLLIVSHDRFLLDKLCDHVFV
                     FETEGRIKDFYGNYTAYYRYRIHELKKQQQARNQLREAEVHPEKEKKASNKPTYKQKL
                     EFGTLEDEISRLEKEKSEVLAALNGGQAESTVLQELSVRYGELEQLIEEKTLRWMELG
                     EIMDT"
     gene            complement(24102..24293)
                     /locus_tag="PKI35_01665"
     CDS             complement(24102..24293)
                     /locus_tag="PKI35_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNX43113.1"
                     /translation="MSTGKVKFFNQQKGFGFIVDDETGSEVFVHVTGVTDRINDNDKV
                     SYDIVEDRRGKKAINVKLI"
     gene            complement(24771..26393)
                     /gene="pckA"
                     /locus_tag="PKI35_01670"
     CDS             complement(24771..26393)
                     /gene="pckA"
                     /locus_tag="PKI35_01670"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNX43114.1"
                     /translation="MAKAINMPGFEIDLNQYGIRDVNEVYYNLSYEELFKHETAPELK
                     GFERGTLTTLDAVNVDTGIFTGRSPKDKYIVRDATSENTVWWSTVGKNDNKPLSPEIW
                     KHLHEVVTRQFARKKLYVVDAYCGANHDTRLNVRFIMEVAWQAHFVKNMFIRPTEEEL
                     LNFKPDFVVLNGSKTVNPDWKEQGLNSENFIAFNLTERMAFIGGSWYGGEMKKGIFSM
                     MNYFLPLKGIASMHCSANMGKNGDVAIFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDD
                     GVFNFEGGCYAKCINLSEENEPDIFHAIRRNALLENVVVKPDGAPDFADASKTENTRV
                     SYPIYHIDNIVKPVSKGGHATKVIFLTADAFGVLPPVAKLTADQTKYHFLSGFTAKLA
                     GTERGVTTPQPTFSACFGNAFLTLHPTKYAVELVKRMEKVNAKAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILDGSIDKAETHILPYFNLEIPGELNGVNTHVLDPRNTYATPELWNE
                     KAADLAKRFIDNFAKYTDNEEGKRLVAAGPAL"
     gene            complement(26840..28291)
                     /locus_tag="PKI35_01675"
     CDS             complement(26840..28291)
                     /locus_tag="PKI35_01675"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HNX43115.1"
                     /translation="MKKRYPAIALILFLMTVIHGIAQDKSGNICYVNSDRMYIRLDKR
                     WSDARKKEISLLYNLDSTLIEQAFAGKILSADSLTWTVEQVNEEVAIISKPFIDGKQP
                     SIAGPEDVLLLDDNLIMNPLLNSFLPSRKKFGINQFRTNSAIQFKNDTTRFFLPGYQE
                     KNQVYFSGSFNNWSTTMLPMKKTGAGWEADLRLAAGKHLYKYIPDGQWIHDPFNKLKE
                     HDGQGGYNSVMYCYNHTFRLDGYTDARNVIVTGSFNNWQKRQLKMNRIPGGWELPIYL
                     EEGTYAYKFIVDGHWMTDPADTNVRQDIGGNRNSFLGIGDTVIFSLNGYDSAQQVFLA
                     GSFNGWNPGELRMNKVPGGWKLPYVMGRGYFEYKFIVDGRWMPDPANPHTTGTGDYIN
                     SYFVLEPNYTFRLKHFSDAQNVIVTGSFNGWQTDNYQMTKQDGYWTFSTYLKPGKYTY
                     KFIVDGTWLLDPDNDAWEENSQGTGNSVLWVEP"
     gene            complement(28299..29312)
                     /locus_tag="PKI35_01680"
     CDS             complement(28299..29312)
                     /locus_tag="PKI35_01680"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HNX43116.1"
                     /translation="MILPFHYRKAYPTVVYSPLLTLILLAFNCQANIPLSGTIPGDTA
                     PHTAFLYPDDDPLGDFLSLTIPLKRAGRLFLIEARIDDQSGNFVFDTGATGLVLNKTY
                     FRKYVSYEKPSSGGSIVGLSGKVYRIMVNRIEISGMHYENIEADLADLGHIENRRGVK
                     ILGLFGLNMIDDFEVIFDARNNQLMLNRTDKSGNRLDIKAFDGKFDFTRKMDIRKNIL
                     LIQGEIGNKVLSFCLDTGAETNVIGSHLPKKVLSTVQISRRSTLQGAGAGKVEVLYGT
                     MNEFKFADHRFGKMETIVTNLEYMSDSYGFPIDGMLGYDFWYEGIFCFNFRKGEISFN
                     LWR"
     gene            complement(29490..32237)
                     /gene="ppdK"
                     /locus_tag="PKI35_01685"
     CDS             complement(29490..32237)
                     /gene="ppdK"
                     /locus_tag="PKI35_01685"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNX43117.1"
                     /translation="MTTDTSKKYVYYFGGKQAEGNGKMKELLGGKGANLADMNLIGIP
                     VPPGFTITTEVCNNYFKLGHDAVVDMIKPQVDEAITKVENALAGPKFGDNKNPLLVSV
                     RSGARASMPGMMDTILNLGLNDEAVETVGVKTGNPRFAWDSYRRFVQMYGDVVLGMKP
                     ASKQDHDPFEEIIDHLKEEKGVQLDTQLSTGDLKELVKRFKAVVKEKTGHDFPVNPWE
                     QLWGAIIAVFNSWMNDRAILYRKLNGIPEEWGTAVSVQSMVFGNMGNNSATGVAFTRD
                     AATGEDIFNGEYLINAQGEDVVAGIRTPQQITIEGSKRWAAQQGISEEIRKNQYPSLE
                     ETMPDVYADLNGIQQHLEDHYTDMQDIEFTIQDGKLWMLQTRNGKRTGGAMVKIAMDM
                     LHQGKISEQEAILRVEPAKLDELLHPVFDPVAIKSAKVMAKGLPASPGAATGQIVFFA
                     EDAETWAAQGKKTILVRIETSPEDLRGMNVAMGILTARGGMTSHAAVVARGMGKCCVS
                     GAGAIEVDYKGRTLKMNGKLYNEGDFISLNGSTGEIYDGKVASQDASLSGDFGKLMEL
                     ADKHSRMHVRTNADTPHDSRVARNFGAKGIGLCRTEHMFFEGAKIKAMREMILADNEE
                     GRRKALDKLLPIQREDFEGIFEAMQGLPVTVRTLDPPLHEFVPHEEANQAEMAAEMGI
                     SAEDVKKKVDSLHEMNPMLGHRGCRLGNTYPEITEMQVRAILEAALNLKARGIEAKPE
                     IMIPLAGTKKELEMQIDIVRKTAEKLFAERNDRIDYLAGTMIEIPRAALTADQIAEVA
                     EFFSFGTNDLTQMTFGYSRDDAGKFLPVYLDKNILKNDPFQVLDQEGVGQLVRMGTEK
                     GRSTRPNLKVGICGEHGGEPSSVEFCHRVGMNYVSCSPFRVPIARLAAAQAAIKEAMA
                     K"
     gene            complement(32379..32903)
                     /locus_tag="PKI35_01690"
     CDS             complement(32379..32903)
                     /locus_tag="PKI35_01690"
                     /inference="COORDINATES: protein motif:HMM:NF025160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HNX43118.1"
                     /translation="MNSFTVLMTLLPVFFMIHDFEEIIFFPSWIKKNKSFLTDRFPVL
                     SRPFLPHIEKMSATAFTVAVSVMFLLICILTYLSVCLEFPYLWLAAFMGFFIHLFLHI
                     AQWIILRRYVPVIVTSFLALPYCICTFSIILQEKLFSVSEIIICTVAGLLILLPVIVS
                     AHKFGVKADEWING"
     gene            complement(32933..33655)
                     /locus_tag="PKI35_01695"
     CDS             complement(32933..33655)
                     /locus_tag="PKI35_01695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HNX43119.1"
                     /translation="MHIHYFQHDDFEDLAYIGRWAEKNGIATSCTRFDRPCPQFPSHS
                     AYDWLIIMGGAPGAYEEDKYPWLSDEKRFIREAIELGKTVFGICLGSQLIAASLGAKV
                     YSNASPEIGFFPVIFNASAAKDEVFCHFPQQLTVMHWHNDTFELPDGAILMASSEATK
                     NQAFRWGRNVFAFQFHFEMNMLAARTLVEKSGQNLPTSRWVQLPEDLLSQSAVLQQNN
                     DLFDIILNEMRSITSHAAKQNR"
     gene            complement(33860..36685)
                     /gene="uvrA"
                     /locus_tag="PKI35_01700"
     CDS             complement(33860..36685)
                     /gene="uvrA"
                     /locus_tag="PKI35_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNX43120.1"
                     /translation="MEYSDIDQLDPRKYIIIKGARVNNLKNLSVAIPRNTLAVITGLS
                     GSGKSSLAFDTLYADGQRRYIESLSAYARQFMGRMSKPEVDYIRGIAPAIAIEQKVNT
                     RNPRSTVGTSTEIYEYIKLLYARIGKTYSPVSGNLVKRDSVTDVVDFALSLSEGAVVM
                     IFSPLLRKNGRTLKEHLTIIMQQGFSRIVSNGEIIKIEDILDNPKNLAEESLQLLIDR
                     FTIDFSDADLPARIADSVQTAFYEGNGACSVRYSVEGNVNTREFSNRFELDGITFEEP
                     SLHLFSFNNPYGACKTCEGFGSIIGIDEDLVVPDKSLSIYDDAIACWKGEKMSEWKHE
                     LIRNAHHFDFPVHTPYYNLSEEQRELLWTGNRYFGGIRDFFAMVESNTYKIQYRVMLA
                     RYRGKTVCPECRGTRLRKDANFVKINGKSVTELVLMPVSESLNFFENLELSEYEKSVA
                     GRILVEIKQRLRFLNNVGLPYLTLNRLSNTLSGGESQRINLATSLGSSLVGSMYILDE
                     PSIGLHPRDTHLLIGVLKGLRDLGNTVIVVEHEEEIIKAADQVIDIGPLAGNHGGELI
                     FQGSYDEITHFENSLTGKYLSGKLKIEIPASRRQWHDFIELRGVRENNLKNINARFPL
                     KVITVITGVSGSGKTSLVKRVLFPALKKIYGGYGERTGKFDRLEGDYSRIAAVELIDQ
                     NPIGRSSRSNPATYVKAFDDIRTLFADQPLAKTRGYKPGYFSFNIEGGRCEECEGEGI
                     VTVEMQFMADIHLTCDACKGKRYKDEALDIKFDGLSIADILDLTIEQAVEFFERHSAK
                     NHTCQRIAAKLKPLADVGLGYLELGQSSDTFSGGEAQRIKLASFLTKGESDVPTLFIF
                     DEPTTGLHFHDIHKLLIALNALVAKGHTVIVIEHNPEIIKSADWVIDLGPEGGDKGGY
                     ILFEGTPEQLACCEKSYTGKFLKPKLV"
     gene            37016..37603
                     /locus_tag="PKI35_01705"
     CDS             37016..37603
                     /locus_tag="PKI35_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX43121.1"
                     /translation="METPFISDQELIGEYLSGNHASLEILINRHKQKVYSYILLVVKD
                     RSLADDIFQDAFIKVINTLRTGTYKEEGKFIQWVMRISHNLIIDHYRKSKRIPTVENT
                     GEDGDIFDHLYIADDSVEDRMIMDQIHQDVRSLIEYLPEEQREVLMMRHYADMSFKEI
                     AEQTDVSINTALGRMRYALINLRKLIEKKELILTT"
     gene            37681..37860
                     /locus_tag="PKI35_01710"
     CDS             37681..37860
                     /locus_tag="PKI35_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43122.1"
                     /translation="MVKTSTIETVISKHKVAGKAHRASATMPSDESVQFLLSYSRALQ
                     VFGNNITGQAYVILN"
     gene            37955..38623
                     /gene="nth"
                     /locus_tag="PKI35_01715"
     CDS             37955..38623
                     /gene="nth"
                     /locus_tag="PKI35_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNX43123.1"
                     /translation="MSDNYTTKERYRRFIEYFSEHMPVAETELHYADPYQLLVAVILS
                     AQCTDKRVNMLTPDFFKKFPDAGTLALASTEDVFELIKSCSYPNNKTKNLIGMARSLT
                     ENFKGIVPSDIEELQKLPGVGRKTANVVASVVFNKPAMAVDTHVFRVAARIGLTKNAK
                     TPLAAEMQLVKHIPENLIPKSHHWLILHGRYVCNARKPKCGECGLNGFCKYFETFGAS
                     LQDK"
     gene            38743..39192
                     /gene="bcp"
                     /locus_tag="PKI35_01720"
     CDS             38743..39192
                     /gene="bcp"
                     /locus_tag="PKI35_01720"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNX43124.1"
                     /translation="MTHLKAGDKAPVFSGTDQAGNPIDSGSFAGKKIVLYFYPKDNTP
                     GCTAEACNLRDNHEDLLQKGYTVIGVSPDNLQSHLKFSEKYQLPFPLIPDPDKKIISA
                     FGVWGPKKFMGKSFDGVHRTTFVIDEYGFIEKIIEQVNTKNHSSQII"
     gene            39353..40354
                     /gene="recA"
                     /locus_tag="PKI35_01725"
     CDS             39353..40354
                     /gene="recA"
                     /locus_tag="PKI35_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNX43125.1"
                     /translation="MNKDEKNEKLSALKLTLDKLEKTYGKGTVMKLGDSAVEEIPAIS
                     TGSIGLDIALGVGGLPKGRVVEIYGPESSGKTTLALHVIAESQKTGGIAAFIDAEHAF
                     DRFYAQKLGVDIENLLVSQPDNGEQALEIADNLIRSGAIDVIVIDSVAALTPKSEIEG
                     EMGDSKMGLQARLMSQALRKLTSTISKTGCCCIFINQLREKIGIMFGNPETTTGGNAL
                     KFYASVRLDIRKQTQIKDGDSAIGNRVKVKVVKNKVAPPFRFAEFDIIFGEGISRYGE
                     LVDLAVEKNIIKKSGSWFSYGDTKLGQGRDAIKKLLADNPELSEEVEKLVRESYKTN"
     gene            40443..41456
                     /locus_tag="PKI35_01730"
     CDS             40443..41456
                     /locus_tag="PKI35_01730"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX43126.1"
                     /translation="MRHILTFSSLLIILTFTLSCKHAGNSDADNKGSDPVLLDLNAKI
                     EKEPKNADLYQQRAVYFMQHEKLNDALTDIDKAIEINPKNPAYFLSLSDIYIMMGKPQ
                     QGLESIEKAITLNDKNVEAYLRKARLYMIMKDYEHCAESVEKVFSIDPQNADAFYLKG
                     YVLGEYGDTTKAIDAYRKAVQSNQLHYDALMQLGSIFVKKDPVMAIGYFENALKAKPK
                     SMEALYNLAMMYQENDKPDQAIRLYGDMLKIDPKNRFALYNSGYVYLVYLNNYTKAID
                     YFSRAFQADSTYADALFNRGYAYELSGDFTRARTDYNKVLKISANYPKAIEGLNRLDT
                     HGR"
     gene            complement(41752..42462)
                     /locus_tag="PKI35_01735"
     CDS             complement(41752..42462)
                     /locus_tag="PKI35_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNX43127.1"
                     /translation="MKDILSSFLNNRTLLFYILLAIALVLTRIPVIGKYFRIVNTMIH
                     EAGHAFITLIVSGQVISINLFADTSGSITTKSKSRFSQFLIAFAGYPVSAIAGYMLMY
                     LIQNQEAIYILFVMLSIVMILMILSIRNTYGFCWAGSFALLNILLIYFDNQTAIYIAA
                     AFFSLIVLTDSLISSLILLAITYNNKKKAGDATNLHKITGIPAMVWAFIFVAISAFMV
                     FLSVKHYFPPLNGLLSNL"
     gene            complement(42465..44975)
                     /gene="feoB"
                     /locus_tag="PKI35_01740"
     CDS             complement(42465..44975)
                     /gene="feoB"
                     /locus_tag="PKI35_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNX43128.1"
                     /translation="MTLFELNVGDTAFIQKVKGRGAFRKRITEMGFVVGKEVTVIKKA
                     PLQDPTEYNIMGYNVSLRNSEAKLIEVSHDASIPRAAYSGTFISATDEPLPEQLTGKT
                     IHVALVGNPNSGKTTLYNYASGANDRVGNYSGVTVDARESRFRFKDYEFVITDLPGTY
                     SITAYSPEELFVRDFIYEKMPDVVVNVIDASNLERNLFLTTQLIDMDIRLIVALNMFD
                     ELQQSGNKLDYVHLGNMLGIPFIPTVASRGKGIEDLFLKIIEVHEGLDKFSRHIHINY
                     GNSIERAISAIQDKIWIPGNREFLDKVSSRFLSIKLLEKDQSIRDYISSFSNREEIIQ
                     TSQREVSKLELDFGEDTESLITDAKYGFIAGAMAETFRENPEHRRSKSDLIDTIITHK
                     FWGIPIFIFIMWATFYGTFKIGQYPMNWIEVGVEWISSAVQTAMPPGMLKDLLTQGII
                     GGVGGVLIFLPNIVLLYLFISLMEDTGYMARAVFIMDKVMHKIGLHGKSFIPLVMGFG
                     CNVPAVLSTRIIESKRDRLITMLINPFMSCSARLPVYILFISAFFTRYQGSILFAIYG
                     LGIMLAVFSALLFRKTIFRQKDVPFVMELPPYRLPTFRSIIKHMWHRAGQYLRKIAGV
                     IMVASIIIWALGYFPQKVKNQDYYNRQLGLITAKYDGLKESMDGNIQAINKLSKQETS
                     EKKTILSVMKAEQQQNSYIGKLGRFIEPVMRPLGFDWKMSVALLTGISAKEVVVGSLG
                     ILYQTGDSNNNDQSLIAKLRSQVYMNGEKEGSPVFTPLVALTFMIFILIYFPCIGVVT
                     AISRESGSWKWALFIITYTTILAYFVSLVISRIGSLII"
     gene            complement(45071..45610)
                     /locus_tag="PKI35_01745"
     CDS             complement(45071..45610)
                     /locus_tag="PKI35_01745"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HNX43129.1"
                     /translation="MNENDEKTALERQAAELLTLATEYFRTLENIGNIDKNDFLSLIH
                     KLCSLLYTKGLLFPETDEPDNAGNERFVTEEEWENIFNSIRAKLADQDSFSYIENIDH
                     PEDIVTGSLAEILTDVYQDMKDFAFLLTKPTQLAFDNAVFEIKRLFYSHWGFRILLAL
                     QVLHSRRYPEVAENKEFDW"
     gene            complement(45738..46094)
                     /gene="rplS"
                     /locus_tag="PKI35_01750"
     CDS             complement(45738..46094)
                     /gene="rplS"
                     /locus_tag="PKI35_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNX43130.1"
                     /translation="MSKKELITYVEKHFLHETSHPAFKAGDTVTVHYKIKEGDKERIQ
                     QYQGVVLQRSGHELNETFTVRKVSGTVGVERIFPVCSPNIDKIEVNKRGAVRRARIFY
                     LRGLKGKKARIKEKRM"
     gene            46317..47477
                     /locus_tag="PKI35_01755"
     CDS             46317..47477
                     /locus_tag="PKI35_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNX43131.1"
                     /translation="MDLSFILNNLGEDIGNGSSPVTPPIYQTSNFYFKTVEDFRQAIL
                     SERDHLIYTRGNNPTIDLLRIKMAALEGAEDALVFASGMAAISSAILAMVKTGDHVIS
                     VQHPYSWTDTLMNSNIFPKFGIEATMVDGTDARNIEQAIKPNTSLIYLESPNSWTFDI
                     QNLGEIGVLAKKHGIITIIDNSYSTPLYQQPLKNGIDLVVHTASKYLGGHSDVVAGIC
                     CGSRELIDKIFHNEFCTLGGIISPFNAWLLLRGLRTLQVRMEHISRTAGEVIAFLQDS
                     GIVEKIYYPFLEGNPGMQTAKNQMTKASGLFSIVLKTRSTAAIEEFCNSLTYWRMAVS
                     WGGFESLIIPSCTFVRPGLYSNLPDNMIRFSVGLETADALIGDLEKNLHLLR"
     gene            complement(47534..47932)
                     /locus_tag="PKI35_01760"
     CDS             complement(47534..47932)
                     /locus_tag="PKI35_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43132.1"
                     /translation="MGLKDSLKKLFKTSKQVGAEKFEDLKEKTADKIDDVVENVAEKA
                     QQATVKAKEIKDDIVEKAEGTLDKIEEKAEKFVDDLELKVRSASKASAEDVKETAAPA
                     QAQPDDQGGNAGTSTGEAKDTAEENAPDIK"
     gene            complement(48022..48699)
                     /gene="trmD"
                     /locus_tag="PKI35_01765"
     CDS             complement(48022..48699)
                     /gene="trmD"
                     /locus_tag="PKI35_01765"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNX43133.1"
                     /translation="MRIDILTIFPEIFSGPFDHSIVKRAIAKGLAEIHIHNIRDYSKS
                     RHRNVDDYAFGGEAGMVMMIQPIADCIEALKERRNYDEIIFLTPDGKQFDQKIANNLS
                     LKENIILLCGHYKGIDERIREHLITSEISIGDYVLSGGELAAAVISDAVIRLIPGVLN
                     DETSALSDSFQDNLLSAPVYTRPADYHGWKVPEILLSGNEKEIRKWQMEQAVTRTKER
                     RPGLISE"
     gene            48810..50516
                     /locus_tag="PKI35_01770"
     CDS             48810..50516
                     /locus_tag="PKI35_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNX43134.1"
                     /translation="MRLFTITLALLLAAISVFSQSSDKEIFVVTFSDKGPDILKSILA
                     PENYLSEKAISRRQKHAVSFDESDLPLYQEYVSQVLSTGVNVLSRSRWLNCIVVEGTK
                     DVQGKLNKIPGVKNVTILKTSGPPDHKNFDKKSIVALKEYNVKGLQKKANGSDVYNYG
                     AAENQISMIKGQYLHNLNYCGQGMTIAVLDNGFNRVDILSAFDSLWINNQILGTKDFV
                     TPGANVFDPDAGSHGTMVLSTMASNIPGTIVGTAPKADYWLIHTEDNAGESYIEEYYW
                     VSGAEFADSVGADVINSSLGYTTFPNSPAYDHTYADMDGNTCVSTIGADMAVKKGILV
                     VNSAGNSGENYDPWQYIGAPADGDSVFTIGAVDGTGIRASFSSKGPTYDGRIKPDIAA
                     QGLDDAVFFWDNGTQQGKIYYGSGTSFSSPIIAGMSACLWQSVPDKSNMDIIQSLRAT
                     ASNSQMPDTLVGWGIPDYEQARLQLSLGTPELPARPGLKVFPNPFSLSFTAILPEALE
                     GRIVAEIISPDGKIMISRHLDSSFPIQSLYMNDLTGLKAGIYILRVIDKNNVFTSTCI
                     RY"
     gene            51324..51860
                     /locus_tag="PKI35_01775"
     CDS             51324..51860
                     /locus_tag="PKI35_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX43135.1"
                     /translation="MKERIQLLIKAKNFTAAQFADEIGVQKSGVSHIISGRNNPSLDF
                     VQKILTRFPEVSTDWLLFGRGPMMLSDVPATFSLPPVAVPASSSGKLADLFSQDIAPV
                     EVSVKPVKVPEKLITDSVPVYKTAIDEPGEKESGKPQENITIPVPKDVKNQEQAKVVT
                     KIIVLYDDKTFSEYHPED"
     gene            complement(51857..52276)
                     /locus_tag="PKI35_01780"
     CDS             complement(51857..52276)
                     /locus_tag="PKI35_01780"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002859435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNX43136.1"
                     /translation="MAGNITFSMIKPDAASAGKIGPILTIFTQNGFSIKAMKYTQISR
                     MQAETFYSVHRGKPFFDGLVEFMSSGPIVACILEKENAVDDFRMLIGPTDPLVAPEGS
                     IRKLYGVSIQQNAVHGSDCDTNAVIESDFFFSRIERF"
     gene            52453..53436
                     /locus_tag="PKI35_01785"
     CDS             52453..53436
                     /locus_tag="PKI35_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNX43137.1"
                     /translation="MPQNILITTHYNPDGDAIGASLALYHFLVKQGHQVDIIVPNELP
                     SFLTWMPGNEKLLIYKQKDDIAHDIIENAGVIFCIDFNALNRVKYYSDQLAAAKGFKI
                     LIDHHLEPSDEFDLTISQIKVSSTSELLYQLIQETGYGDCVDLPVAECIFVGIMTDTG
                     SFSYSCEKPDTFRITSELIRIGVKVEKIHRLVYDTYSESRMRLLGHCLSQRMKVIPGL
                     STAYIWLSKEDLEAFDYQPGDTEGVVNYALSIENIVLAALFTERDDRIRISLRSKGQF
                     SVNDFARKNFMGGGHRNAAGGDSFLSLEETMTKFESLLEGYRDELMNNRKS"
     gene            53449..53976
                     /locus_tag="PKI35_01790"
     CDS             53449..53976
                     /locus_tag="PKI35_01790"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNX43138.1"
                     /translation="MMKKIYFLAFLFLLSGCGQKEGNRPEKSDAADLNETLIKANKQV
                     VLSEDQQIDDLLERYGWNMTETGTGLRYLIYKRGNGPSAQKGQQATIRYNLRLISGDI
                     VYSSDDLGPKQFTIGSGGVESGLEEAILLMHLGDQAKVVIPSYLAFGLIGDQDRIPPK
                     STLIYDIELIKLTNN"
     gene            54006..55370
                     /locus_tag="PKI35_01795"
     CDS             54006..55370
                     /locus_tag="PKI35_01795"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNX43139.1"
                     /translation="MKKILLVIPVALLLTLVLSSCGKHAGYKKTDSGLYYKFYTQNED
                     SVKADTSKIATLLISYRTTVNGKDSVIFDSKTRNVPFEIKLMKPVYPGDIFEALSMMH
                     KGDSASFILNAGDFFLKTAQAPQVPEGIDSTADMIFNIRMLKVQTMEEYQMDKAQQLT
                     RQKDNAAKSLQDYLTKNNVTTAPTASGVYIIHQKPATGRAIKKGDFVKIHFSVTLIDG
                     KKLFSTHDNGTPMTFEFGQPFDTKGFDEGIGAVKEGETARFIVPGEMAFGEQGRRSMN
                     GEELIAPYATIIYDVEVLDVSTKAEHEKQVAEETAAKKKAADLAKNNEPSLIKKYVAD
                     NKITVKPTASGLYYIERVKGKGKKAEKGMKVQMNYTGRLLDGTKFDSNLDRKPLKPFE
                     FTLGQQEVIPGWDEGVALMNEGGKATFIIPSKLAYGEREMGGKIKAYSPLVFEVELVK
                     VLGK"
     gene            complement(55462..57489)
                     /gene="uvrB"
                     /locus_tag="PKI35_01800"
     CDS             complement(55462..57489)
                     /gene="uvrB"
                     /locus_tag="PKI35_01800"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNX43140.1"
                     /translation="MNFKLTSEYVPTGDQPEAIRQLVAGLNEGDASQVLLGVTGSGKT
                     FTIANVINETNRPTLILSHNKTLAAQLYGEFKQFFPENAVEYFVSYYDYYQPEAFIPV
                     TNTYIEKDLSINEEIEKLRLSASSSLMSGRRDVIVVSSVSCIYGIGNPDDFTKNVIRI
                     EVGNHLSRNKLLYALVDALYSRTEIDFTRGKFRVKGDTVDIFPAYTDVSYRVIFFGDE
                     IESIHSFDPLNGKMIESVPGLVLFPANIFVTSKDKMKDALWDIQSDMVKQVEYFKSLN
                     RNLEAKRLEDRVTYDVEMLRELGYCSGIENYSRYFDGRMPGARPFCLLDYFPDDFLLV
                     IDESHVTVPQIRAMYGGDRSRKQTLVEYGFRLPSAMDNRPLKFEEFESMYPQVIYVSA
                     TPADYELQQSQGVVVEQLIRPTGLLDPLIEVKPSLNQMDDLLDEVEQRIQKGERTLVT
                     TLTKRMAEELTKYLTRLGIRCQYIHSDVDTLDRIDILRDLRMGAFDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSERSLTQTAGRAARNLNAKVIFYADKITDSMRRAMEET
                     ARRREKQMAYNQKHNITPTAIIKSAEQIMGQTTVADAKAKPQAAAYIENDKIDVAADP
                     VIHYMPTEQLKKMLDQTRRAMEKAAKELDFIEAARLRDEMLSYKDLLEKRK"
     gene            complement(57518..58666)
                     /locus_tag="PKI35_01805"
     CDS             complement(57518..58666)
                     /locus_tag="PKI35_01805"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNX43141.1"
                     /translation="MHLESQKSNKKKRSFGTGLTLFLNWIVVLFLLLAYLSRYISPET
                     FWPLAFFGLFYPVILIINIFFILFWLVYLKRYFLISLIAILLGYSYLISFFSIGNHHD
                     KTGDSEDIKVMSYNVRLFNLYGWKGNTHREMRKAIFDLIKTESPDILCIQEYYSGKGL
                     KADYADTITKAINTKYGFAAFIESEKKDLPVGLATFSKYPILHSHKILFENSDVNFCI
                     VNDIIVHSDTIRVLNAHLESVRLGKEDFSLVTEWNSLNDPNKDYNKGSRIILRKIKSA
                     FARRSKQIPALEEIINSSPYPVVFCGDINDTPVSYAYQKLTRSLNDAFVMAGNGIGQT
                     YAEVLPFLRIDYILTSKSISVNDFYVIGKPYSDHYPIMARIKPGSHPD"
     gene            complement(58704..>59267)
                     /locus_tag="PKI35_01810"
     CDS             complement(58704..>59267)
                     /locus_tag="PKI35_01810"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNX43142.1"
                     /translation="LGGIAGGIVFMAAFNFFPVFQQSLGQALALGASASVLAIVIMIS
                     FMVPDYTVNLFLIGQVKIKYIAIAMIVTDILMIRSGNAGGHFAHLGGALWGFTYMLLL
                     RKNIDPARLLEFKRIQGQHRGTARMKKVKFRKVHTSGRPLSDEEYNARRATDQKKIDE
                     ILDKIAKSGYSTLSKEEKEFLFKTSRK"
BASE COUNT        16165 a        13183 c        13589 g        16330 t
ORIGIN      
        1 aaaaatagta tacaacggaa tggtaaacat gaataaggat ttgattaatt gacttctgtg
       61 cgtttttttt gctgccattt taaaggtcac gaaagtttct gtttaaccgg agccggattc
      121 ctaaatttat aattcccgcc ggatgatcat gcggtaatgg gcgatgtgaa tcgctgaagc
      181 ggttcaggat aaccatcagc cattattttt ttggccggcg tcgttgatgt ttccttaact
      241 ttgaacaggg aaagaaaaac ggttatttcc cggattcgtt ttcataatag taagatatgc
      301 agcttgtaag caattacgaa aatttataca aagcccttgg cgggaaagtc aggatggtag
      361 ctgtttccaa aacaaagcct gttgaaatga tcagggagtt gtatgatgcg gggcaaaggg
      421 atttcggcga aaaccgggtt caggaactta ccgcaaaaag cgctgtcctt cctgccgaca
      481 ttacctggca ctttatcggg caccttcaaa ccaacaaggt aaggtttatc gttccgtttg
      541 tcgggtacat ccattctgtt gacagtctga aacttttatc tgaaatcaac cgggaagccg
      601 caaagatcaa ccgggtgatc aattgcctgt tacaatttca cattgcaacc gaagaaacaa
      661 agttcggctt atcgttcgaa gaagcggcag cattgctcaa taatccggat accgccagga
      721 tgcagaacgt aaggatatgc ggcataatgg ggatggctac atttaccgat aataaacagc
      781 tcatccggaa tgaattccgc cagctcaaat cccatttcga taagctgaaa acatcacatt
      841 ttaacgacga tccggctttc cgggagattt cgatgggaat gtcgaatgat tatgagattg
      901 ccattgagga aggaagcacc atggtaagga tagggtcgtt gattttcggc gaaaggtagc
      961 tgcaggatta cctttcggcc ccggttcatt ccggttgttc ccgaataaaa acggaggcgg
     1021 tggcctccgt ttttattaag ggggtccgac ttttattaaa tggcccaatt catcagcagg
     1081 aacaagtaat atacggcggc tgagataatc atcgagacgg gtatcgtaag aatccaggcc
     1141 cagagaaggt tgatggtgac gccccagcgt acggctgata agcgctttgt catgccaacg
     1201 cccataatgg cgccggtaat agtgtgggtg gtactcaccg ggatgccgaa agcttctgtc
     1261 ccgaacagta aaagagcccc ggcgctttct gcagcgactc cttcgaaggg ggtgagtttg
     1321 gtgatcttgc ttcccatggt ttttacgatt ctccatcctc cgagcagagt tccgcatcca
     1381 atggcagtat aacatccgag cacgatccag tggggtacgg catgtatctt gccattgaca
     1441 agggtaatat gggcccagtg aggtatctgt gaaggatcag cgccatgaaa ataaacgata
     1501 agcgttgccg ctaggatgcc catcaccttt tgggcatcgt tgccgccgtg tcccaggctg
     1561 aacagggccg aagtgaaaag ctggaccttt ttaaatactt tttccagttt atagggattg
     1621 gcctttcgca agatattcag cagtgtgacg gatatgacat atgaaaagaa catacccaga
     1681 aagggggcca ggaagatgaa agcggctatt tttacgatca ccccggtatg aacaattccc
     1741 cagccggcgt gggcaacggc ggcgccggca aatcccccga caagggtatg cgatgaactt
     1801 gaaggaatgc ccaggtacca ggtcagcaaa ttccagatta tggctgcaat gatcccggca
     1861 agaatcacgt atagatcaat ggcgttggta tcgacaactt tagcgattgt atcggcaata
     1921 ttcagttcga agatggcata cgcaaggaaa ttgaaaaatg ccgcccatat tacggcctga
     1981 aaaggggtga gcactttggt ggatacaatg gtggcaattg agtttgccgc atcgtgaaaa
     2041 ccgttgatga agtcgaatga aaacgcgagg actattatga tgattaaaag tgtaaagctc
     2101 ggcatccgct tactgattag tgatgtgttg tttgaaatcc gatgagttgc agagaatgaa
     2161 aggcagcatt acgacatctt aatcatgatg ccggagaaaa catcgccgac atcatcacac
     2221 ttatcaatgg ctttttcaag ggaagcaaga atgtcgcgct ttttgataag gtcaatggca
     2281 ttcgtttcat tctcaaacaa atctgccaga aatgcctggt aaatgtcatc tacttcattt
     2341 tctatttcgc tgatcctgat acatgagtct ttgtgccgtg caagcccggc aacatctttc
     2401 atgtcgcgga tcacattaag tatttccttg ttggcatcgt gaataagatc ggcgatggta
     2461 acaaattcag gagggaaggt gtgtaatttg taaaaatgga tgcgcttgga tgtggaatgg
     2521 ataaaatcaa ccacgctgtc gagcgtcgaa gctaattcat gaatatcttc acggtcaaac
     2581 ggggtgataa aagtgctgtt aagctcatcc atcaggtcac gcgtgatatt gtcaccgttt
     2641 ttttcggctt tcttgatggc gacaataaat tcttcacgtt tttgcatgtc gttttcacgg
     2701 ataagcttta ccagcagttc cgatgctacc accaggtttt cgcctgcctg tataaacaac
     2761 gggaaaaact tcttgtcttt cggtacaagg aagccaaaga ttctgtcgag tttcataatg
     2821 atggatgtag aggtggatga acaaaatgaa ttgcgaaatt aatcacaatg caggatatat
     2881 cggcagggat gaatcaagaa tttctcaaac ataatccaat tgaaggacca gaaatcacgc
     2941 gaatgatttc tgcgattgaa aaccagggga aatccggtgt taacgacggc cggtcaaacc
     3001 gccgagcaac ccccttatga gggttcggcc aatttccctt ccgatggtcg aggtaaccga
     3061 tcgcccgaca gtttttgtca ggtcggtaaa aatatcccct ttcgaatgat tgcgtctttc
     3121 ttctgccgcc gcttctttct cagcttttgc cctttctttc tcagcttgta tcctttcttt
     3181 ttcaagcctt tcctgttctt cttccattct tatttgttcc tgctctttat taatacgggc
     3241 ctgaagtatt tcaaaagccg actcccggtc tacttctttt tcatatacgc ctgcaatcaa
     3301 tgagctgcgc atgatacgtg accgttcctc gggtgatacc ggcccaatct gggcttgggg
     3361 aggaagcact tttgccctct ccaccggtaa gggaatgcct ttttcgtcca ggaacgaaat
     3421 caacgcctct ccaaccccca gttcggtaat gacctgctca acatttactt ccgggtttgg
     3481 acgaaatgtt tcggctgctg tggcaacggc tttctggtcg cggggagtga aagccctcaa
     3541 cgcatgctga actttgttgc cgagttgtcc caacacaacg tcgggaacat caatggggtt
     3601 ttgtgtgcag aaataaatgc cgacgccttt tgaacggatg agtctgacaa cctgttctat
     3661 tttatcgagg agcaccctgg gcgcttcatt aaacagcagg tgagcttcgt caaaaaagaa
     3721 aacaagcttt ggtttatcca ggtctccggc ttcaggaagc ctttcataca attcggaaag
     3781 gaggtagagc aggagggtgg aataagcgcg gggcgacatc atgagtttgt cggcggccag
     3841 aatgttgatc tgtcccaatc cgttcgaatc ggtttgaatg aaatcaaata tgtcgatggc
     3901 gggttccccg aaaaatttat cggcgccctg ctgttcaagg gtgatcagtt cgcgctggat
     3961 agccccgatg ctcgcaacgg aaatgttgcc gtatgcggtt atgaactgat cacggttatt
     4021 tccgacatac tcgcacatct tcttcagatc cttcatgtcg agaagaagga ggttgttatc
     4081 gtcggcgatt ttaaaaatta tggtcagaac gctgctctgg atatcattca ggttcatgag
     4141 ccggctcaga agaagcggtc ccatttccga tatcgtcgtg cgcaacgggt gcccctgttc
     4201 accgtacaaa tcccacaggg ccaccggaaa tccccggtgc atatagccct ggagctgcaa
     4261 agcctgaacc ctttcaagaa ttttgggatt atcaacgccg gtattgctta tgccggaaag
     4321 gtctcctttt atatcagcca tgaacacagg cacgcccttc aggctgaatg cttcagccag
     4381 cacctggagg gtaacggtct tacccgttcc ggtagctccg gcaatgaggc catggcggtt
     4441 tgccattttt gggctgataa acaattcctt taatgaggct gatttggcga tcagcatgtt
     4501 tccttcacta gtcagcatga tgtatgtttt tttcgggaaa gtatcttttt acaaatatat
     4561 aaaacctatc atccggcaga tacctttact gttaaatttc agcaaaacgc ctgttttgac
     4621 acacaaaaac ggatgtaagg gctgaaattc tgatactggt ttcagacgca ggttttcctg
     4681 ccgacgatgc agcgaaataa tgaaatttta actcactgga ttactttggc gggccggaaa
     4741 aggcgcagcc aggtttattg cctgatgata atatactcac cggtttccat taaccggata
     4801 atagttgaag gctttgactg ggaaaacgtt tcctgtcggt atttaacaat gtaatcaaca
     4861 ttgctgatga tatcctttga aatatggctg aaaattacgg gcgattcctc acccgaaata
     4921 ttcgccgatg tagatacaat aggtttcccg aaccggctga tcaggttaat gcaaaattct
     4981 tctttaacaa tgcggattcc aatggtccca tccccggcga taacattcga tgccaggttt
     5041 ttggcattgc tgtatatcac agttacagga ttttcgatgc atttcagtaa gtcgtaagct
     5101 acatccggaa ttttatcaac atagttgttc agttgttcaa aagagtcgat caggataatc
     5161 aggctttttg attcaatccg ttgtttgatg cgatatattt tttcaaccgc gttatggttg
     5221 gtggcatcgc atcccagtcc ccagatggtg tcggtaggat acaagatgac acctcccgat
     5281 tggagcgttt tcagggcgtt ttcaatatct tcttgaaatt cagggtcttt cataaggcta
     5341 aatttgagag aagttgacgg gtatcgattg aggttgcgca gttaaaatga ttttcgggac
     5401 attcccggtg accatgaagt ccgcatggac ggcatgcaag gctgagcggg gtttcaacaa
     5461 taaatgcgtt atcgctgagc gggccaaagc caaactgagg aacagtggaa cagaaaattg
     5521 ccgtgatatt tgcgttcatg gcggaggcca ggtgcatggg ggatgagtcg ttcacataat
     5581 tcatcaaggc ccctttcatc agggcggcgg attgcagcag cgtcagttta ccggccaggt
     5641 tataacaacg gtctttaccg gattcgttga tgatattgtc gcaaagatct ttatcatccc
     5701 ctgatcccaa taacatgacc ccgatcgttg ccggaagttc tttgatgaat aaaatccacc
     5761 gggaggcagg atactgtttg gtgaaccaca atgacgcagg agcaacacaa atataccggt
     5821 ccgacgaata ggcttcaacg catttaatat ctgatgccgt cggatacagg cggggccttg
     5881 ccggcaggtt gtcggtgaac ggcgcgatga gagcctggtt cctggctact tcatgagctc
     5941 cgtcttttgc cgggatgtga tgttcagcct gatgggtgaa ccacctcgaa aatggattct
     6001 tgttgaaccc tgctgtgtag ctggctttgg aaagtaccgt aaataaaccg gtcgaagcaa
     6061 accgttgcag gttgatgatg cggtcgtaac gtatttcccg cacttgtttc agcaatttcc
     6121 aaaggttctg atacttgttt cccgatttat cccaaaccag cagctcgtta ataaatggat
     6181 gttccccgaa aagggcttca tttccttttt ttaccatgaa atcgagtgtg gcatcaggga
     6241 aatgatggtg cagtttctcc agcaatgctg ttgccaggat gacatcgccg atggatgcag
     6301 tttgtatgat cagaaatcgg ggcaagaagg tttgagttta gggctgtagt aaaaaaatca
     6361 gttggttaag cgatgttgta ttcgcggagc gcttcattaa gcgatgtttt taaatcggta
     6421 gaaggcttgc gtttgccgat gataagggcg caggtgacgt tgtatctgcc tgccggaaac
     6481 tctttctcaa tggaaccggg gatgaccacc gacctgggtg gaacatagcc tttatattca
     6541 accggttcgc tgcccgaaac atcgatgatc tttgttgagc cggttatcac gacattggcc
     6601 ccgagaacag cttcggaccc aattcgcgcc ccttcgacaa tgatacagcg ggatccgata
     6661 aaacaatggt cttcgatgat gaccggggct gcctggacag gctcaaggac accgccgatt
     6721 ccaaccccgc cgctcaggtg aacgctggcg ccaatctggg cgcaactgcc caccgtagcc
     6781 caggtatcaa ccatggtgcc gggtccaatc catgcgccga tgttaacata agaaggcatc
     6841 atgataaccc ccggagaaat atatgctccg taacgggcta cggcatgggg aacaacccgg
     6901 atacccaatg attcataacc atgctttaaa gggattttat catgaaattc caacggcccg
     6961 gcttctatcg tctccatctg ccgcaacgga aaatacagga taacagcttt tttaacccag
     7021 tcgttgactt tccaggtatc caactccgga taggctacgc gaaggctgcc cttatcgagc
     7081 atctcaatga cgctgtaaac tgcctgttgc gtttcagcgt ttttcaacag gctcctctca
     7141 acccaggcgt tgtcaatcag tgttttaagt tcattcatat cggggaaaat gtactccctc
     7201 aaagataggc aagaattgtg catgaaaaga tgtaaaatat gctgtgatca gttgaaaacc
     7261 ttgcaggtta acttatcagg cgaattgatt ttctgggtaa ctttgcagca aaaatgagat
     7321 gggcagggta atagccatcg actatggaca aaagcggacg ggacttgcag ccactgatga
     7381 gcttcaaatg atcgcgtcgc cgctttgtac ggtccctacg gtaaaagtga tggattttct
     7441 ccgggattat atccaaaggg aaaaagttga tgtgctggtg gtgggagaag cggttcagat
     7501 gaatggttca ccttcctctt ccgccgaatt tatcgagcct tttgtccgtc ttttaagccg
     7561 cactttcccg ggaattccgg tcgaaagggt cgacgagcgg tttaccagta aaatggcaag
     7621 ccggaccatc ctgcattcag gagcgaagaa aaaggaaagg caggataagg ggctgattga
     7681 cagggtaagc gcagcgatca ttctgcaaag ctaccttgaa agaaaaggaa tggattgatg
     7741 aactttccgg gatattaccc cgtttatgat aaaaacaagg acgtttaatt tatgatttta
     7801 ccgattgtag catacggaca tcctaacctc cgtaaaattt cactggatat tactcctgag
     7861 tatgaaggac tgaacgaact gatcgccaac atgtatgaaa ccatgtacta ttccagcgga
     7921 gttggtttgg cggcccccca ggtaaacagg cagataagaa tttttgtgat cgatgcaacg
     7981 ccttatggca aagaacatcc tgaagcgtca acatttaaaa aagcttttat caacccgcgc
     8041 atcgtcgggg aaaaaggaga agaatgggcc tttaatgaag gatgcctgag tatcccggat
     8101 attcatgaag acgtacttcg tccggccgat gtacacatta tttatcgtga tgaagagttt
     8161 gttgaacatg atgaatggta ttcaggcgtt atagccaggg ttattcagca tgaatacgac
     8221 cacctggaag gaaagttatt tgtcgataaa attcatcccc ttcgtaagat gatgctcaag
     8281 cggaaattga atgacatttc aagggggaac attgatgttt catacaaaat gatatttccc
     8341 caaaagaaga aatactgata aaacctgtaa ataatattgg tcagaacaat gaaaaaaatc
     8401 atttcaggaa catttgcgat cctgctcatc ttgctgttct cctcgtgtgg ctcatcacct
     8461 gaaaaaatgt cggcaaaaat cgcaaaactt gagaaggaag ttaaagagca atcgcctgcc
     8521 gatacggtaa aaatcaacga actggtagag gcttacagta aatacgcttc aaagtatccg
     8581 aaagacacac tggcccctga gtatcttttc agggccggag gaatgtgcct gagcttcaat
     8641 aaaccgtcaa aggcaatttc gctgtttcag caggttgaga aagattatcc ttctttttca
     8701 agggttggtg aatgcctgtt catgcaaggc tttatttacg aaaacgctct cggcgatttt
     8761 tcaaaagccg gtagtattta ccgggaattc attgccaaat atcccgggca tccgcttgcc
     8821 gatgatgccg aaatgtcgct gaaatacctg ggccggccgg ccgaagatat tgtacgcgat
     8881 tttgaaaaga aggaagccga cagtgctgcg gtagtgcagg aaaagtaaat tgaacggaaa
     8941 gcccctgatt aacagggttt atacttattc tgacggttac tttaccgcgg gactaaagtc
     9001 gttcagcagc atgttcctta atccggcagc ttttatatcg accgaaagct gtccatccct
     9061 gcaataggat atatttcctg tttgctccga aacgatgatg gaaatggcat cggtgcgttc
     9121 cgaaacgccc agcgccgccc ggtgacgcaa gcccagatca tttggcaggt ccgaactgga
     9181 agataccggg aggatgcacc gtaccgcttt aatacggttc agcgaaataa ccatcgcccc
     9241 atcgtgcagc gggttgttct tgtagaagat actctcgatc aaggggtcgg taatcgtagc
     9301 atctatggca acgccggtct ccgtgaattc ggtcaggtca ttttttcgtg agactataat
     9361 aagagcgccg gttttcgtca tagccatgcg ctggcatgcc tgaaccacgg ggtctatatc
     9421 cgaaaggttc tcatcttcca gctttatctt ccagaagaga aaccgcgaac ttgtcttttt
     9481 gaaaaatccg ggagaaccga tagccagtag aaacttacgt atctcgggct ggaaaacgac
     9541 aatgaaagca ataaaaccca cgctgataaa agctcccagc aggtcgctca gcaaatccat
     9601 tcccaatgct ttcactacac gccaaagggc gaagatagcg agtatcccca ggaaaatatt
     9661 gacggcggca gtccctttca gaagttggta caaccagtat aacagaaggg ctaccaacag
     9721 aatatccacg atatccagga cccgcaccga gagaaatgac aacataaaat cagttttaca
     9781 acaacaaatg aattgtgcaa atatacttat ttaccggcct tcaggtattc gttcacgatg
     9841 cttatggttt ccacagcctc ttttacatca tgtaccctta agatgtcagc tcctttaagc
     9901 aaagccaggg tattcaggat gcttgtgccg ttcagcgctt cgttgggcga tatgtttaat
     9961 aatttataaa tcattgattt ccttgaaaca ccgacaagca ccgggcatcg aagttcagca
    10021 aagcgatgga ggttacggag cagttcgtaa ttatgctgaa tggtctttcc gaatccgaac
    10081 ccgggatcga taacaactga accgacgccc atttcctgaa gtttttgcag ttttgcctga
    10141 aggctgtcga aaacttcacc tgcaacatcc cggtataccg gatttacctg catagttgcc
    10201 ggagttccct gcatgtgcat cagcacgtaa gaagcaggca atccggccat cacggcaaac
    10261 atgttttcat caaggtcgcc gcctgaaata tcgttgatca tgttggcgcc acacgcagcc
    10321 gttgcccggg caaccgacga acgaaaagta tcgatggaga aaattatccg gctgtaacgc
    10381 tttacaagtt cattcaggac aggaagcagc ctttgcagtt cttcactctc ggtaacctcg
    10441 ggagcgcctg gcctggttga aacagcgccg atatcaatga ttttggctcc ttcggcaatc
    10501 atctggtcag catgggccag gcatgcgtcc aggtttttat attttccgcc atcgtaaaat
    10561 gaatcgggcg tgacatttag gatgcccata actaccggtt ccgacaggtt aagcaattta
    10621 tcgccgcatt tcagcgttct ggaatgtgga aaaaccgtat ttttaggctt catttcaagc
    10681 atataaacat ctgtttatga tccaccacaa agatattgat tctatggcag ctaggacgtc
    10741 ggaacaattt gacgccgtta ttgactcctg tcgcaggatt ttccttgata agatgcatga
    10801 ttatggcact gcctggcgaa ttctgcgtcc ctcttcgctc accgatcaaa tctatatcaa
    10861 agccagcaga atccgcagcc tacaggaaaa agggcacagc agggtagggg aaggaatatg
    10921 cccggaattt atggggattg taaactattc gatcatggcc ctgattcaat tggaactggg
    10981 ttcatcggaa gaagcccgta tggagtctga aaaggttgaa acactgtata ataccaatgc
    11041 cgccaaggcc aaatcgctga tgacggataa gaatcacgat tacggggaag cctggcgcca
    11101 gatgcgggtc agttcgttag ccgatattat cctgatgaaa cttctccgca tcaagcaaat
    11161 tgaagataac aacggggtta ccctgatcag cgagggcctg gatgcaaact acctggatat
    11221 tatcaattac tccgtctttg ccctgattca actgaatgac caaaaataaa ctgtttgtta
    11281 attaacgcta ttttaaaata tgaagatact tgccaacatc agccgactgc ttacagcttg
    11341 tgtttttctg ttttccggat ttgttaaagg tgtagatccg ctgggatttt cctataaaat
    11401 cgaggactat tttattgcct atcacatgga ctgggccata cccctcgcct taactctcgc
    11461 catcatggcc tgcgccctcg aattatcaat cggcgtcatg ctgttgctga aattacgtat
    11521 gaaaccggca tcctggttac tgttgctgat gatgtcgttt ttcacggtac taacgttttt
    11581 tgatgccgtc tataatccgg ttcccgattg cggctgtttc ggggatgcca ttatacttac
    11641 aaactggcag actttttaca agaacattgt ccttatagtg cttgccatta ttgtattcag
    11701 ttacaggaaa aagttcagca acggcttttc tccctctgcc caatggatga ttacagggat
    11761 agtggcagcg ctgtttattt cattttcggt atattgttac cgtcatcttc cggtgattga
    11821 ctttaccgaa tggaaaaaag ggcataaact gtatgccgaa aatccggttc cggtgaaata
    11881 ttttgtgaca taccggaata atgccacagg ggaacagaaa gaattcctgt cgccgaatta
    11941 tccttttgac gacagcacct ggatggctca atgggtattt gtttcccagc gtgttgaaga
    12001 cccgaataaa tactatggca agtcattggt tataaccgat acgctgggga atatccttac
    12061 ggaaactgtt accaggaatc ctgaatttca actcattgtg aatgcctatg acctttcgtc
    12121 cgcaaacagc caagccttca gcgcaatcga taaattttgc ctcaaagcag ccggtgacgg
    12181 cgtgtcatcg gtagcgctgg ttgcctcgga gcctgcggat attgtaaact ttgtccatag
    12241 gaacaaacta tcaatagcgt tttaccagtc ggatgacatt gttctgaaaa ccatggtccg
    12301 ctcaaatcca gggattatgt tactcagaaa cggaatcatc attgacaaat ggaactggcg
    12361 cgatttaccc gattacgaaa agttcaaagc cgaatttctg acccaaaaag aaccgtcata
    12421 acacctggcc ttttctatgt tgcaatatac gctcaaaaga ctgctttatg gcttcctggt
    12481 tttactgggc gttattacgc ttattttcgt gcttttcaat acgctgcccg gcgatccggc
    12541 caggatgatc ctggggcaac gcgccgacct tgcatccgtt caagccatca accgtgatct
    12601 cgggcgcgat aaaccgctga cggtccagta ttttaacttt ctgaatgata tttccccgat
    12661 ctcagtccat aactgtgcaa attccggcag ttactggttt cttgatccgg aaaaatacgc
    12721 atcagcgata cctttgttta atattggcaa cgctcattta ctggtgttga aacaacctta
    12781 cctccggcgt tcataccaga gcaagcgacc tgtttctgaa attcttgccg acgcttttgt
    12841 tccgaccatc atcctggcga ctgtcgcaat gctgcttgct acactggccg gattgttaat
    12901 cggcatcttc agcgccctgc acaaggatac ctggttcgat aagacagcac tgttgctgtc
    12961 ggtgctcggc atgtcgttgc cgtccttctt tgccgccata ctcatagcgt ggatattcgc
    13021 ctttgttctg tcggatatta ccggacttag catgttcggc agcctataca gtgtcgatga
    13081 tttcgggaat ggggaatata tcagtctaaa aaacctgata ctgcctgttt ttaccttagc
    13141 catcaggccg ttggctatca tcaccgagtt aaccagaagt tcgatgctgg atgtcctcgg
    13201 gcaggattat atccgcactg cgcgcgccaa aggcctcagt gaacgaaaga ttattgtcag
    13261 gcacgcgctt aaaaatgcaa tgaacccggt attgaccgct gtgtcaggct ggtttgcatc
    13321 gctgattgca ggtgcagttt ttatcgaata tgttttcgat tggaaaggca tcggcgtcgt
    13381 cattgtagat gccctcgaaa aattcgattt cccggttgtg atgggcgccg ttttatgtat
    13441 cagcgtgttc ctggtcatca tcaacatctt ggtcgacctg gcatacgggc tgcttgatcc
    13501 aaggatcaag tattcataat tgcttaaaaa tgaaattaat agattgaatt aactaataaa
    13561 atcattgaac catgagaaac aaaattgtag caggtaactg gaagatgaac acgacgttcc
    13621 aggaagccga ggatctgtta tacgaaataa ccgacctgct gaacgaacgc gggaaaaatg
    13681 atacgatgat catcatctgc ccgccttccc cgtacctgga aatggccagt gatataggcg
    13741 ttgaaaacga cttttttgtc ggggcccaga atatcaacga ccatgaaaaa ggtgcatata
    13801 ccggtgaaat atcagcaccg atgattagtt cgatggggat tacccatgtc atacttggac
    13861 attccgagcg cagaacatat tacggagaga ctgatgctgt gatcaaagca aaaattgacc
    13921 ttgccctggc gaacgacctg gttcccatat attgttgcgg cgaagtgctg gccgagcggc
    13981 aaaaatcaaa tcattttgaa gtggtccgcc agcaaataag cgaagcttta tttcacctgg
    14041 atgcggatgt gatttctacg gtcatcatcg cctacgagcc ggtctgggcc attggaacag
    14101 gcgtaacggc cactcccgct caggcacagg aaatgcatgc ctttatccgg ggcctgatag
    14161 gttcgaaatt tggggatgac gtggcagata aaatcaatat tctgtatggc ggcagttgta
    14221 atgccggaaa cgccgctgaa ctcttctcac agccggatgt tgatggcggc ttgatcggcg
    14281 gggcttcgct caaggccgga gactttgtga ccattgtaaa ttcattcagc gaataacaag
    14341 gaagtcctga tacggtaaaa aacagcatgt tatggattat tatgaatttc ggttcaggct
    14401 gaaacctgtt gatgacgcag gcgatatgct tgtcgccatg ttaggcgata ttggcttcga
    14461 gagtttcatc gaagaggata acgtcctgaa agcctatatt cctgtcgcag ggtatacgga
    14521 agaatgtatc agcagccttt caggggatca gatcatccgg tggttcgcca gctacgaaac
    14581 agaacttatc agggacaggg actggaatgc cctttgggaa agcgggtaca gcccggttgt
    14641 gattgacgac aaactaatca tcagggcgcc gtttcatccg aaaagggatg aaattcccct
    14701 ggaaatcatt attgagccga aaatgtcgtt tggcaccgcc catcatgaga caacgagact
    14761 catgctcagg tatatactgg agcttgacat ggagaataaa agcttgcttg acatgggatg
    14821 cggcacagca gcgctggcca ttcttgcacg caaatcaggc gccaatccgg tgacagctat
    14881 cgataacgac gaatgggcct ataacaattc gatagagaat atcgctaaca acaacatcac
    14941 tgatatcggg gtatacctgg gagatgccgg tctgctggag aaaaagcgct atgacataat
    15001 cctggccaat attaaccgga atatcctgct ccgggacatt ccggcatatt cagcatgcct
    15061 tgatccggga ggattgttgg tcatgagtgg tttttatacc ggcgatctgg attctgtttc
    15121 gcaacgcgcc cgcgaatcgg ggttggaact attgtctgac cgtgaagaaa acaaatgggt
    15181 agcggcttgt tttatcaaac gatgatccta aattaatcgg agatgaaccg gaaaaggctt
    15241 tgttgcgttt ttatattcct gttgactgcg ggttttgtgg atgcccagga ggaaccagcg
    15301 aagacttttt cggcagatac atcgaaattt gtcggcgatc ttacggcttt ttttgccacc
    15361 gtcggggaag gcgatgcaaa agaagcgcgg gatgtattat cggattttac ctatcattgg
    15421 acttcaaacc tgctgacacc ggcgcaaaaa aaacaggttc agtcggtctg caacgccatg
    15481 cttgaacaaa agttcaaggt ggccccgcat tttatagagt atctgaaaag catctcactg
    15541 atggtaaggc ggaatatccc cgcaaaggct ttcgatacac attatctcag cgttaactat
    15601 tgccttgagg ggaaaaacag tgcacaaaat ctggcggctt atctcaccca gacgaatctg
    15661 ctgctcgccg attatgcatt ccggctgaat aacacagaag catggcgtat gagggatgcc
    15721 cgctttgaat ttgattttga taccgtgccc gtattcaggt tctcatcggc aacattaagc
    15781 tgcatcgtac gcggcgacag tacaaatatt tacgatacca gggggctcta ttttcccatt
    15841 acacagcaat ggttagggaa tggaggaaaa gtatactggg accgcacagg gcttggacgc
    15901 gacagcgttt ttgcagaact ttcaacatac cggatcaatc ttcgaactac atcttattcg
    15961 gctgatacgg tatcttttgt gcaccggggc tattttgcct atgcattagc cggaaggttc
    16021 gaagagaggg ttattacgga tgtagcccct gaacgagctt cttatccccg ttttacggca
    16081 tatgccggtg acctatacat tcacaacctg ttcaggaata tcgattatga aggcggattt
    16141 gccatggaag gagcaagagt attgggcggt ggcgtaaata aatccacggc gataatccac
    16201 gtacaacgaa acaaccttcc gttcattgaa ctgagatccg gcgaatttgt aatcaggcct
    16261 gaccgctttg tggcaaacag ggccaatgca acgattttta tcggcaatga ttcaatttac
    16321 catcccggat tgttggtgag gtacatcaag tccaatgatg aatttgtttt cacacgcatg
    16381 gaggaaggcc tctcgcaaag cccgtttttt aacagctatc acctggttga tatgtatccc
    16441 gaagctatct attggaaaat ggacgatgat aaactcagcc tggaagcgat acggggcccc
    16501 aggagcaaag gcgatgcgct ttttgaatcg gccgatttct tttcacaata tcggtacgac
    16561 cggatgcagg gtatggatga agttaatccg gcaaatctgg tttcaaccta tatccgcaag
    16621 cattctaccg atcgttttta tgccgatgaa tatgccgatt atatcaggat gccgctggaa
    16681 caggtgaggg tacagttgat caggctggcc aatgccggtt ttatcattta tgacatggat
    16741 aacgatttcg ttacggttaa accccgttta aatgaattcc tggcagcccg cggagggttc
    16801 aaggattacg atatcctaca gttctattcc accgttgaaa aaggaagtaa tgccatcctt
    16861 gacctgaagt cccttgacct gaccctgtat ggcgtcaggc aggtaatgct cagcgattcc
    16921 caatatgttt atgtcgtacc ccgcgatggt aaaatcgtga tgaaaaaaaa tcgtgatttt
    16981 acatttacag ggcgtgttca tgccggtttt ttcgaatttt attccaacga gtgttctttt
    17041 gattatgacc atttccgcat gaacattccc aagatcgatt caatggcgat gtatgtgtat
    17101 gcctggcatc ctgatctgaa cggttaccag ccgttaatcc gggtcaaaaa tgtcatctcg
    17161 gatatgagcg gcgaccttca gatcgatgat ccgggaaaca agtcggggaa aaagtcattg
    17221 aaaaatttcc ccgtttttga gagcaaggat atctcctatg tttatttcga taaaaagagc
    17281 acggtgcagg gagtttacaa gcgtgataaa ttttattaca gtgtcgatcc atacattatg
    17341 gacagcctga atcatttacc gacggcaaac ctgaaatttg aaggccacct gaataccggc
    17401 gatatgttcc cggttattga cgaaccgctc agggtacagg aagactattc gctggggttc
    17461 ataaaaaagc taccggcagg cggtttgccg gtctatgccg gctcgggggt atacagcgat
    17521 acattgaagc tgagtaacaa cggcttgcgt ggatcgggta agctaaccta cctggcttcg
    17581 acttccgaat cacgcgattt cctctttacg cctgattcca cttctgcaac actcaagaat
    17641 ttcaacctga aagaaacagt tgcttctgcc cgatatcctc aagtggctat cagggaatca
    17701 agagtgaagt ggttgcccgg aaaagatgtc atgacggtta gcaacctgcc tggcgacagc
    17761 atcagccttt tcaacaacca cgcgttgctg tccggttcgt tggcgcttac tccggaaggg
    17821 ctaagaggcc ggggaaaaat gacctttgaa gatgcccagg tcaattcaaa cagttacgat
    17881 ttcattaaca atgccttcac ttccgataca tcggatttcg cgctgtacac ccctgaccat
    17941 aaatcgcagg tgctcagtgt tcatgtcttt cgcaccgata tcgattttga taaacgttta
    18001 ggacatttta cggcttccgg caagggctcc aggatggaat tcccggtcaa taaatatgtc
    18061 agccttgttg atggctttga ttggatgatg gataagaaag tattgcaact cctgaccaaa
    18121 acaaccttta ccgaagatgc ttatcaccgg atgaacaggg atcagttgct caatattcca
    18181 tccggtgatg acaggttcat ttcaagcgat tacgcacagg attcgttgag cttttttgcc
    18241 atgcgggcaa attatgactt gacatccaat gttttacaag ttcttgatgc aagaattatc
    18301 aaggtggccg atgccgccat ttttcctgta aacggagagg tgaccattgg cagggatggg
    18361 gtgatgaatg agctgaaagg cacaaccatc ctggccaata ccgatacaaa ataccatgag
    18421 ttgtataatg ccgatgtcgt gatcagttcc aaaaaccatt acggcggaaa aggcctttac
    18481 gattatgttc ccgccgacgg cgaccttcag accctgcaga tgagcactat caacgtgaat
    18541 tcggaacggc aaacctatgc tactgccacg gtaacggaaa ccgacaattt taaattgagc
    18601 aatcattttg gcttcgaagg cgatatcgaa ttaaatgcgc ccgataaaat gttaagcttc
    18661 caggggacct tcaatcttgt gcatgaatgt gaagcattag gtcatgaatg gattaaaata
    18721 aatgcgaaga tcgatcctgc gaatatcatg attccggtca gtaacgatat ggaaaacagg
    18781 ggaaacggaa acctccgcac ctcaatactg tattcgtcaa ccaagcaggg gctgttgccg
    18841 gcattcttcg agaagccgca gaacatcaac gaccaggata tcatagcggc taccggatat
    18901 ctcatattcg atacgttgag aagagagtac cggattgctc cgaaagaaaa actggaagac
    18961 gtttccaaac ctggcaatat gctggctttc aatacaaatc aatgtattct tgaaggaagt
    19021 ggcaaaatta atctggtcgg ggatatgggc cgtctgacca tgaaatcagc cggcaaaggc
    19081 gcctatttta tgattcctga ttcagctact ttcatggtta caacagcctt cgatttcttc
    19141 ttttccgaag aagcgctcaa ggttttctca accgacctga aaatcgccga tctgaaaggc
    19201 atggacgttg cggcgacttc ctatagtttt cccttatatg aatttctgga taccgccatg
    19261 gctcagaaac agatggcaga gctaacctct ttcgggcaat tcaggaaatt cccggaagaa
    19321 ttgaaccata cgctgatgct ttcagaaatg aaactggcat ggaatccgga gctgaggtca
    19381 tttgtcggga agggaccggt cggtattatg agcctgggta aagaaatggt aaacaaatat
    19441 gtggaatcgt acctggaaat cgggaaacgc cggaccggcg atatgttcac gttctatttc
    19501 gaactcgaag accggcattg gtattatttc agttatgcca atggcatcat gcaggctatt
    19561 tcatccaata aggattttaa tgataaactg gtcggactgg acgaaaaaga ccgcacgatt
    19621 aaatcgggtg acgggcaacg aagctatcag ttcatcgttt ccaacaacga aaagaaagga
    19681 tctttcctgc gtaagatgaa acaggctttc ggggaataga tggaagaaat atccatcgga
    19741 atccgaaccc cgtcatttta cagcaacaac cagcccgcag gctgccgggc cggttcttat
    19801 ttcatttcgg gcgcagtaca tgtcggatgc ccgccctctg gtttcctgtt gaacgaaccg
    19861 gggaattatc cggaccaggc ctgttacccg gattttttat gctaagcagg tgggtttggg
    19921 cctaaaagag aggaagggcc atttttacct ccagaaaaat agttacattt ggccatgata
    19981 tcatattttc cgattgtatc tggagcggtt atcgcttatc tgatgggttc gttttcctcg
    20041 tcggtttgga tcggacgggc atttttcagt gttgatgttc gcgatgaagg cagtcataat
    20101 gctggcgcta ccaatacaat acgcgtcctt ggcgtaaaag ccggaataat tgttttactg
    20161 ctcgatgccc tgaaaggttg gggcgccgtg gcgttgtctg cattatttgc cggcagtttt
    20221 ttttcgggtg aacggctggt ttattataaa attttagcag gcctgatggc ggttttgggg
    20281 catgttttcc cggtatatac cggattcagg ggaggaaaag gagttgcaac cctggttgga
    20341 gtagttgtag cgctgtttcc gaaagttttc tggattgtcc tggtatggtt ttttaccgtc
    20401 tttttggcaa ccggttatgt ctcgctggca tccgttacca gcgccattct tctgccgttt
    20461 atctcatttt tcatttttca cgaaaagtca atggccatca ttgtattgtc cattctgata
    20521 gccgtattta tccctgttac acatcataaa aatatccgca ggctgatcag gggagaggag
    20581 tccaaactga aactgtggaa gaaaaagccg tgagaagaag ctaaaaccgg gaaccggacc
    20641 ggtgaagcat gagtgaagac attcagcatt taaacaggct gcataccggc aagtataaag
    20701 cagacaagct tttaaccgct atcgccaacc atgattcttt tcttcctgtt gattacctgt
    20761 tgaaaaatcg ttgaggggtg ctttcatttc tcgtttaaga ttcatacttt tacctgtgta
    20821 acaaagatcg ttttaattaa ttgaaataaa ataggttatg aaatttataa tcaatagcca
    20881 gaccttgttg aggaagcttc agtcgctgag cggcgtgctc aacagcagta atacacttcc
    20941 gatcctcgat gacttcatgt tcgaacttac ggatactgca ttaaacatta ctgccacaga
    21001 ccttgaaaca acaatggtca tttcactgga gccgtctatg ctggaagagc ccggaagcat
    21061 aaccattccg gcaaaactgc tgctcgactt cctgaaatac ctgcctgatg ttccgctcac
    21121 tttttccatc gacctggcat tacaggctgt tgaaattgtt tcaagcgacg ggaaatatca
    21181 tcttaccggc cataatgccg aggattatcc caaactgccg gaacttacgg aaaccgccag
    21241 gattgagatg ccatccgatc ttcttgctgc cgggattaac aaaaccgtct tcgctaccgg
    21301 aaacgatgaa ctgcgtccgg cgatgggggg cgttttcttt gagctgactc ccgattacct
    21361 tacttttgtt gctaccgatg cgcataaact ggttcgttac cgccgcaccg atgccaaagc
    21421 cgaagattcg gccagtttca tagtgccgcg caaaccgctg aacgtattga aaaacctcct
    21481 ggctactgat attgcccagg tgacggtgga atataacgtt acgaatgccg ccttcgaatt
    21541 ccagaatgtc aggctttatt gcagactgat tgaaggcaaa tacccggctt atgaagctgt
    21601 tattccgcgc gacaaccaaa accatctcac cgttgaccgc caggttttca ttaacgccgt
    21661 gaagcgcctt tcgccttttg caaatcaaag cacacatcag atccgtctta gaatcagcgg
    21721 ccaggagctg gtaatgacag ccgaggatat tgactattca aacgaaggtt ctgaacgcct
    21781 gaactgcact tatgacggag aggatatgga aattggcttc aactcgaagt ttgtgctcga
    21841 tatggtgaca aacctcgaat ccgatgagat ttgctttaat ctggcaggcc ctacacgtgc
    21901 cggaataatc cttcctgtta acaatgaaaa caaggacgaa gatatcctga tgcttgttat
    21961 gcctgttatg ctggctcagg gctgatcctg gtataagttc tcaaagagga agccggtgat
    22021 atccggcttt ttctttatgt gcctttcagg aatcagggaa gccggagaat gcttcccagg
    22081 cgccacatct tttaaccttt aggtgtccat gatttcgccg agttccatcc agcgcagggt
    22141 cttttcttcg atcagttgtt ccagttcgcc ataccgtacc gataattcct gtaaaacggt
    22201 agattccgcc tggccgccat tcaatgctgc cagcacttcc gacttttcct tttctaaccg
    22261 gctgatctcg tcttcaagcg ttccgaactc cagtttttgt ttataggtgg gtttatttga
    22321 agcctttttt tctttttccg gatggacctc cgcttcccgt agctggttcc tggcctgttg
    22381 ctgtttcttt agttcatgga tccgataacg ataataagcc gtataattgc catagaaatc
    22441 tttgatccgg ccttctgttt cgaaaacaaa aacatgatcg cataacttat cgagcaggaa
    22501 acggtcgtga gatacaataa gcaggcaccc cgggaaatcg ccgaggaatt cttcgagtac
    22561 ctggagggtg aaaatgtcaa ggtcgttggt aggttcgtca aggatcagga aatttgggtt
    22621 ccgcatcagt tgcagcaaca ggtacaaacg gcgtttctct cctccgctca gggcgctgaa
    22681 ataagcatgc tgaacggcat atgaaaaatt aaactgataa aggaattgcg cagccgacag
    22741 gctccctttt ttcattacga tctcctcggc tacatctttc acgatatcga taacgcgttt
    22801 atcctcgacg ggttgcaacc cctcctgggt gtaatatgcc gtcaccacgg tctgacctgt
    22861 aaccacactg cctttatcgg gccgcatgtt tccgctgatg atgttgagca gcgttgattt
    22921 tccggcgccg ttcaaaccta ccaccccgat tttttcccct cttttaaaga tataggagaa
    22981 gtcgtcgatg atagtgttat cgccgaactt cttacagata ttatttactt caatgatttt
    23041 tccgccaagc ggattagcat taacgttgaa ttttatcgtt ttttcttccg gcttccggct
    23101 tactttctct ttcaattcgt cgaacgcttc gatgcgggct ctgctctttg ttccccgggc
    23161 cttcggcatt ctcctgatcc attcgagttc ggtcctgtac aggttggtgt tcttttcgag
    23221 tccctgctcc tcggccgact gccttagagc tttcttctcc aggtagtagg aataattgcc
    23281 cttgtattgc cataaagaat cctgctccag ttctacgatt gagtcgcaga cattatccag
    23341 gaaatacctg tcgtgcgtaa tcaggaagag ggtgagcgat tcgcgcgaaa gatattcttc
    23401 gagccattcg atcatttcaa aatcaagatg gttggtgggc tcatccatga gcagaaggtc
    23461 aggggtgccg ataagggcgc cggccagcgc aatctttttt atttgtccgc ctgaatggtc
    23521 cccggccggt ttggagaggt cactgatccc gaaacgtcct aaaatttcac ggactttgga
    23581 ctcataatcc caggcctgta aggcatccat cctggccata ccggcgtcaa gggcgcgctg
    23641 atgggcttca ctgtgttcat gcctgaacct ctcaagggct tcctcataat catggatggc
    23701 attcatgaat tcgttatcgc cggtaaacag gatatcaagc acacttttcg aagcttcata
    23761 ctgcggattc tgtctcaggt aggcaacgcg tgtatcgcgg cggatcgtaa cgcggccttc
    23821 atcggcagca tcctggcctg caatgatgtt catcagcgtg gtcttaccgg ttccgttcct
    23881 ggcaataagg gcgattttct gccctttgct gatgccaagg cttatatcat gaaaaagaac
    23941 tttttcgcca aaactctttg aaagatgctc aacctgtaga atattcatga aatgcccgga
    24001 gtgtttagag tgtaatattt caggcgcaaa attagggttt taattgcagg cataaaaaaa
    24061 gcttcccgaa ggaagctttt taaggtagtg caatgtatta cttatataag cttgacattg
    24121 atagcctttt ttccgcgacg gtcttccacg atatcgtagg aaactttgtc gttgtcgttg
    24181 atgcggtctg taacaccagt aacgtgtaca aaaacctctg agccggtttc atcgtctacg
    24241 ataaagccaa agcccttttg ctgattgaaa aattttactt ttccggttga cataatattg
    24301 taattattat ttgcggcaaa ggtaagaata aatgattcga tttttaactc tgcataaaat
    24361 ttttatcgat ttatgtatag atgtactctt aaaatttcat caattgtatg tgctatttag
    24421 ctataaaacc aattatatca attagttata caatttatca gggattagaa atttagtata
    24481 aaatttgcgt attcagaaaa aatcagcctg acaaaccggc tttttacctc gaaacacact
    24541 gttttttatt gacaagggtc atgatattgt tacagatata aagcgcagct ataccaatct
    24601 ggcccctgac gacaaatgaa aaggttgtct caaaagagga tatttcaatc cttacacctg
    24661 tcaatgttcc tcatgattta atccggacga taaatcccgg attctgattg caaggcaggc
    24721 ttttgagaca accctgggaa ataatggata atatttgtga gcagatcgga ttacaaggca
    24781 ggacctgccg caaccaaacg tttaccttcc tcgttatcgg tgtatttggc aaagttatcg
    24841 ataaagcgtt tggcaagatc ggcagctttt tcattccata actccggcgt ggcataggtg
    24901 ttacgcggat cgagaacatg ggtgttaacg ccgttcagtt ctccgggaat ttcaaggtta
    24961 aagtaaggca gaatatgagt ttcggcctta tcgatggaac cgtcgaggat agcatcaata
    25021 attgcacgcg tatccttgat tgagatacgt ttgccggttc cattccaacc ggtattaacc
    25081 aggtatgctt ttgcatttac cttttccatg cgtttcacca gttctacggc gtatttggta
    25141 ggatgcagcg tgaggaaagc gttgccgaaa caagccgaaa atgtaggttg tggagtggtg
    25201 acgccacgtt cggtgccggc cagttttgcg gtaaagcccg aaaggaagtg gtatttggtc
    25261 tgatcggctg ttaatttggc gaccggcggt aatactccga atgcatcagc agtaaggaaa
    25321 atcaccttgg tggcatgtcc gcctttcgag accggcttta cgatgttatc aatgtgatag
    25381 atgggatagg agacacgggt gttctcggtc ttggaagcat ctgcaaaatc aggcgcaccg
    25441 tcaggtttca caactacgtt ttcgagaagc gcattgcggc ggatggcgtg aaaaatatca
    25501 ggttcatttt cttcactcag gtttatacac ttggcataac agccgccttc gaaattaaaa
    25561 actccgtcat catcccatcc atgttcgtca tcgccgatca actgacgttt agggtcggta
    25621 gaaagggtcg ttttcccggt tcctgataag ccgaagaaaa tagctacatc accgttttta
    25681 cccatgttgg ccgaacaatg cattgaagca attcccttca gcggcaggaa gtagttcatc
    25741 atggaaaata ttcccttttt catctctccg ccgtaccatg aaccgccaat gaaagccatc
    25801 cgctcagtaa ggttgaatgc gatgaaattc tccgaattca ggccttgctc tttccagtcc
    25861 gggttaacag tttttgaccc gttcaggact acgaaatccg gcttgaaatt gagtagttct
    25921 tcttctgtgg gacgaatgaa catattcttg acaaaatgcg cctgccaggc cacttccatg
    25981 atgaaacgca cgttgagcct tgtatcgtgg ttcgctccgc aataggcatc cactacatat
    26041 aattttttac gggcaaactg gcgggtgacg acttcgtgca ggtgtttcca gatttcaggg
    26101 gataatggtt tgttgtcatt ttttccgaca gtcgaccacc atactgtatt ttcgctggtg
    26161 gcatccctga cgatgtattt atctttggga gaacgaccgg taaagatgcc ggtatccaca
    26221 ttcacggcat caagggtggt aagtgttccc cgttcaaatc cttttaattc aggggcggtt
    26281 tcgtgtttaa agagctcttc ataggagagg ttgtagtaaa cttcgtttac atctctgatt
    26341 ccatattgat tcaaatcaat ttcgaacccg ggcatgttga ttgcttttgc catttctgat
    26401 aaggttgtta tataattggt ttaaaaagaa tatattatgt atgtcataac aagcatatga
    26461 gcatacgggg ttagttgaat caaaattatt aatttatatg ggatgaaact tcttttcgga
    26521 atatttcatc agttattaca gacagtttat caacagagca acgcctgaag cctgaatatg
    26581 agctttgaat ataaaaagta ccatgcaaaa agattcaaat aatttgctat tccgtttcga
    26641 aaaaaaggaa cagagaatca gccttgatat aatcccgttc atgtttcttt taaagcaggt
    26701 caatcggttt agtttttaaa cagggccgtc ctgaaaaatg aagccggcaa attttggtat
    26761 aaggtggcgc atgaacgggc cggcaggctt aatgcgggac ttttatcagc atctttccta
    26821 aaccaaagga ttctgtcact cagggctcaa cccacaaaac agaattgcct gttccctgtg
    26881 agttttcctc ccacgcatcg ttatccggat cgagaagcca ggtgccgtca acaataaatt
    26941 tataggtgta ttttcccggt ttgaggtacg ttgaaaaggt ccagtagcca tcctgctttg
    27001 tcatttggta attatcagtc tgccagccgt taaagcttcc ggtaacaatt acattctgag
    27061 catcggagaa atgtttaagc ctgaatgtgt agtttggctc cagtacaaaa tatgagttga
    27121 tataatcgcc ggttccggta gtatgaggat tggcaggatc gggcatccat cttccgtcca
    27181 caatgaattt atattcgaaa tagccccggc ccatgacata cggcaatttc catccgccgg
    27241 gaactttgtt catcctgagt tcaccgggat tccacccgtt gaagctcccg gccagaaaaa
    27301 cctgttgtgc tgaatcatag ccgtttaaac taaaaatcac tgtgtcgccg atcccgagaa
    27361 atgaattccg gtttccgcca atgtcttgac gaacgttggt atcggccgga tcggtcatcc
    27421 aatggccatc cacgatgaat ttataggcat aggtaccttc ctcaaggtat atcgggagtt
    27481 cccatcctcc gggtatccgg ttcatcttaa gttgcctttt ttgccagttg ttgaaacttc
    27541 cggttacgat aacgtttcgt gcatccgtgt aaccatcgag cctgaaggta tggttgtaac
    27601 aatacataac cgaattataa cctccctgac catcatgctc cttaagtttg ttaaacggat
    27661 cgtgtatcca ttgcccgtca ggaatatatt tataaaggtg tttcccggct gcaagcctga
    27721 ggtcagcctc ccaacccgcg cctgtttttt tcatcggaag cattgtcgta ctccagttat
    27781 taaaagaccc cgaaaaataa acctggtttt tctcctggta tcccggcaga aaaaaacggg
    27841 ttgtatcgtt tttgaattgt atggccgaat tagtccgaaa ctggttgatg ccgaattttt
    27901 ttcgggaggg cagaaaggaa tttaataaag ggttcatgat caggttgtca tcaagcagca
    27961 ggacatcctc cggccccgca atcgatggct gcttcccgtc aataaacggt tttgatatta
    28021 ttgcaacctc ctcgttaacc tgctccaccg tccaggtaag actgtcagca ctaagaatct
    28081 ttcctgcgaa agcctgttca atcaaagtgc tgtcaaggtt gtacaacagc gaaatctcct
    28141 tctttctcgc atccgaccag cgtttgtcga gacgtatgta catccggtcg ctgttcacgt
    28201 aacaaatgtt gccggatttg tcctgggcta tgccatggat gactgtcatc aggaaaagga
    28261 tgagagcaat tgccggatag cgttttttca tggttatctc acctccataa attgaagctt
    28321 atttctcctt tgcggaaatt gaaacaaaaa atcccttcat accagaagtc gtaaccgagc
    28381 attccgtcaa tgggaaagcc atagctgtca ctcatatact caaggtttgt cacaatggtt
    28441 tccatttttc cgaaccggtg gtctgcaaat ttgaattcgt tcatcgtgcc gtatagcacc
    28501 tcgacttttc cggcgccggc gccctgtaag gttgaacgac ggctgatctg gactgtgctg
    28561 aggactttct tcggcaggtg actgccgatc acattggttt cagcccccgt atcaagacaa
    28621 aagcttaata ccttgtttcc gatttctccc tgtatcagta atatgttttt tctgatatcc
    28681 atcttccggg taaaatcaaa ttttccgtca aatgccttaa tgtcaagacg atttccggat
    28741 ttgtccgtgc ggttcagcat caactggtta tttcttgcat caaagattac ttcgaagtcg
    28801 tcgatcatgt ttaacccgaa cagcccgaga attttaacac cccttcggtt ttcaatatgg
    28861 ccgagatcag cgaggtcggc ttcgatgttt tcgtaatgca ttcccgaaat ttcaattctg
    28921 ttgaccatga tccggtaaac cttaccggat aaaccgacga tgctgccgcc ggatgatggt
    28981 ttctcgtaac tgacgtattt ccggaagtag gttttattaa ggaccagccc tgttgctccg
    29041 gtatcgaaga caaaattacc agactggtcg tcaatccggg cttcgatgag gaacagacgt
    29101 ccggcgcgtt ttaatggaat agtcagtgag aggaaatcac ccagagggtc atcatcaggg
    29161 tatagaaatg ccgtgtgagg cgctgtgtca ccggggatag ttcccgaaag aggaatattt
    29221 gcctgacagt tgaatgccag caggatcagg gtcagcaacg gggaataaac gactgtcgga
    29281 taagctttcc tgtaatgaaa cgggagtatc atatcggtaa tatgtttata cgaagttata
    29341 acaaattgat ttataaaaca gtaaaaaata gtgttggcat tacacaattg tcctgaatac
    29401 tgtatgctcc ggtttgtcct gattccggga taaaaaagag gccatcctta ataagggatg
    29461 gcctcagaaa ttatatagcc ggatacttgc tatttagcca tggcttcttt aatagcagcc
    29521 tgggcagcag caaggcgggc gataggaacg cggaaagggg agcaacttac atagttcatc
    29581 ccaaccctgt ggcagaattc aacggatgaa ggttcgcctc catgttcacc gcagatacct
    29641 actttcaggt tcgggcgtgt tgagcgtcct ttttcggtac ccattcttac caactggccg
    29701 acgccttcct ggtcaagaac ctggaacggg tcgttcttca ggatattttt atcgaggtaa
    29761 accgggagga atttgccggc gtcatcgcgt gaataaccaa aagtcatctg ggtaaggtcg
    29821 ttggtcccga aggagaaaaa ttcggccact tcagcgatct ggtcggcagt aagggcggcc
    29881 cggggaattt cgatcatggt gccggcaaga tagtcgatcc tgtcattgcg ttcggcaaac
    29941 agcttttcgg cagttttgcg aacaatgtcg atctgcattt ccagctcttt tttcgtgcct
    30001 gccagcggga tcatgatttc gggttttgct tcgatgccgc gggctttcag gttaagggcg
    30061 gcttcgagga tggcgcgcac ctgcatttcg gtgatttcag gataggtatt tcctaaacgg
    30121 catccccggt gacccagcat ggggttcatt tcatgcagcg agtcaacttt tttctttaca
    30181 tcctcagcag aaattcccat ttcggcagcc atttcagctt ggttggcttc ttcgtgggga
    30241 acgaattcat gcaatggagg atcaagggtg cgaaccgtaa caggaagtcc ctgcatggct
    30301 tcaaatatac cttcgaagtc ttcgcgctga atggggagca gtttgtccag agctttacga
    30361 cgcccttctt cattgtcggc gaggatcatc tcgcgcatgg ctttgatctt ggctccttca
    30421 aagaacatgt gctcggtgcg gcaaagtccg atacccttgg cgccaaaatt gcgtgctacg
    30481 cggctgtcgt gcggcgtgtc ggcattggtg cggacatgca tgcgcgagtg tttatcagcc
    30541 agttccatca gtttgccgaa atcgcctgat aacgaagcat cctgtgatgc aactttacca
    30601 tcataaattt caccggttga accgttcagc gaaatgaagt cgccttcatt gtaaagcttt
    30661 ccgttcattt tcagggtgcg gcccttataa tcaacttcta tagcgccggc gccggataca
    30721 cagcatttgc ccatgccgcg ggctacaacg gctgcatgcg aagtcatacc gccgcgggca
    30781 gtaagaatgc ccatagcaac gttcataccg cgaaggtctt cgggggatgt ttcaatacgg
    30841 acaaggatgg tctttttacc ctgagcagcc catgtttcgg cgtcctcggc aaagaaaaca
    30901 atctgaccgg tggcagcgcc cggcgatgcg ggtagtcctt tggccatcac cttggcgctt
    30961 ttaatggcaa cgggatcaaa aacagggtga agcaattcat cgagcttggc gggttctacg
    31021 cggagaatgg cttcctgctc gctgattttc ccctgatgaa gcatatccat ggctatttta
    31081 accatagcgc cgccggtgcg ttttccgttt cgcgtctgaa gcatccacag tttaccatcc
    31141 tgaatggtga actcgatgtc ctgcatatca gtataatgat cttcgagatg ttgctgaatg
    31201 ccgttaaggt cggcatatac atcaggcatg gtttcttcaa gcgaaggata ttggttttta
    31261 cgaatttctt cgcttatgcc ctgctgggca gcccaacgtt tcgaaccttc gatggtgatc
    31321 tgctgcggag tgcggatgcc ggctaccaca tcttcaccct gggcgttgat gaggtactca
    31381 ccattgaaaa tatcttcgcc tgtagcggca tcgcgcgtga aagcaacacc ggtagcggaa
    31441 ttgttaccca tgtttccgaa caccatggac tgtacgctga cggctgtgcc ccattcttcc
    31501 ggaataccat taagcttgcg gtaaagaatg gcgcggtcgt tcatccaact gttgaaaacg
    31561 gctatgatgg ccccccagag ttgctcccag ggattgacag gaaaatcatg ccctgtcttt
    31621 tcttttacaa cagctttaaa gcgttttacc aattctttga gatccccggt gctgagctgt
    31681 gtgtctaact gaacgccttt ttcttctttc aggtgatcaa taatttcttc aaaaggatca
    31741 tgatcctgct tgctggcagg tttcatgcca aggacaacat caccgtacat ttgtacgaaa
    31801 cgacggtaag agtcccatgc aaagcgggga tttcctgttt taacgccgac ggtttcaacg
    31861 gcttcatcat tcaatccgag attgaggatg gtgtccatca taccgggcat cgaagcgcgg
    31921 gctcctgaac ggacagatac cagcagggga tttttattat ctccgaattt cgggccggcc
    31981 agagcatttt caacttttgt aatggcttcg tcaacctgag gcttaatcat atcaacaaca
    32041 gcatcatgtc ccagcttgaa gtaattattg cagacttcgg tagtaatggt aaatcctgga
    32101 ggaacgggta tgccgatcag gttcatgtcg gcaaggtttg cgcctttgcc gccgagtaat
    32161 tctttcatct ttccgttgcc ttcagcctgt ttgccgccga agtaataaac gtactttttt
    32221 gaagtgtcgg ttgtcataaa agatgttctc cttctttatt gtttattaat attctcaatg
    32281 atttacgcat ttcgttttaa gggtgcaaaa gtacgaaact tagggattga attttgaatg
    32341 gaaaacaaga aaatcagata tctgaatctg cccgccattt atccatttat ccattcatcc
    32401 gcttttaccc caaatttatg tgctgataca atgacaggaa gcaaaataag gagtcctgcc
    32461 actgtacaaa tgatgatctc tgaaacacta aacaacttct cttgcagtat aatactgaag
    32521 gtgcagatac aatagggaag ggccaggaaa gaagttacta tcaccggcac atatctcctt
    32581 aaaatgatcc attgggcgat atgaaggaac agatgaataa aaaatcccat gaaagccgct
    32641 aaccacaaat agggaaactc caaacagact gacaaatagg ttaatatgca tatcaggagg
    32701 aacataacgg aaacggccac ggtaaacgcc gttgccgaca ttttctcaat atgcggcaga
    32761 aatggcctgc tcagaaccgg aaacctgtca gtcaggaagc tcttgttttt ttttatccac
    32821 gatggaaaga aaatgatttc ttcgaaatca tggatcatga aaaatactgg caacaaagtc
    32881 atcaatacgg tgaagctgtt catgtttcag taccggtttg gttaatgtta attcatcggt
    32941 tctgttttgc ggcatggctg gtgatacttc gcatctcgtt gagaataata tcaaacagat
    33001 cattgttttg ctgcaggacg gctgattgac tcagcagatc ttccggaagt tgaacccacc
    33061 gggatgttgg cagattctgt cccgattttt caaccagggt cctggctgcc agcatattca
    33121 tttcaaaatg gaactggaag gcgaacacgt ttctgcccca gcgaaaagcc tgattttttg
    33181 tggcttccga cgaggccatc aggattgctc cgtcggggag ttcaaacgta tcgttatgcc
    33241 agtgcataac cgttagttgt tgcgggaaat gacagaaaac ttcgtctttg gctgcgcttg
    33301 cattgaatat aacagggaaa aaaccaatct caggactcgc gtttgagtaa actttggctc
    33361 ccagcgatgc agcgataagc tgtgaaccaa ggcaaatacc aaacactgtt tttcccaatt
    33421 caatggcctc ccggataaaa cgtttttcat cggacagcca gggatacttg tcctcttcat
    33481 aagcgcccgg ggccccgccc atgatgatta accaatcata ggcgctatgg gatgggaact
    33541 gtggacaggg ccggtcaaag cgggtacagg aagttgcaat gccgttcttt tcagcccaac
    33601 ggccaatata ggccaggtct tcaaaatcat catgctggaa gtaatggatg tgcatcggaa
    33661 ttcaaatgta ggagttttat atgcagggac cgttatgtta aaagaggaaa ttgaagttcc
    33721 agactatgcg tcctgtatgc cgttccggaa tcgggaggca gtccgacgct atcagggacg
    33781 gcaaccgcgc ttcctttttg catctttcat ttagccatcc atcaccggcc tcagtacgga
    33841 tgagtttccc tattccggat tatacgagtt tcggcttcag gaattttccg gtataggact
    33901 tttcgcagca ggccagttgt tccggagtgc cttcaaaaag gatatagcca cccttatccc
    33961 cgccttcggg accgagatca atcacccagt cggcgctctt gatgatttcc gggttatgct
    34021 caatcacgat aaccgtgtgc cctttggcaa caagcgcgtt caaggctatc agcagcttgt
    34081 ggatgtcatg aaagtgaagg ccggttgtcg gttcatcaaa aataaaaagg gtaggaacat
    34141 cgctctcgcc tttggtaagg aacgaggcca gtttgatgcg ctgggcttca cctcccgaga
    34201 aagtatcact cgattgtcct aactccagat atccaagacc tacatcagcc agaggtttga
    34261 gtttggctgc aattcgctgg caggtgtgat tcttggctga gtgcctctcg aaaaattcca
    34321 ccgcctgttc gatggtaaga tccagtatat cggcaatgga caatccgtca aatttgatat
    34381 ccagcgcttc atccttgtat cgtttgcctt tacaggcatc gcaggtgagg tgtatatctg
    34441 ccataaactg catctcgaca gtcacaatgc cttcgccttc acattcttca catcgcccgc
    34501 cttcgatgtt gaatgagaaa tatccgggct tataccccct tgtttttgcc agcggctgat
    34561 cggcaaaaag ggtgcggatg tcatcaaaag ctttgacata agtggccggg tttgaccttg
    34621 aggaacggcc aattgggttc tggtcaatca gttcgacggc agcaatacgg gaatagtcgc
    34681 cttccagcct gtcgaatttg ccggtgcgtt ctccgtaacc gccatatatt tttttcagcg
    34741 ccggaaataa taccctttta accagggatg tcttgccgga tccgctcaca cctgtaatca
    34801 cggttataac cttgagcggg aaccgggcat taatattctt caggttattt tcccgaacgc
    34861 cgcgcagttc aataaaatcg tgccattggc ggcgagatgc aggtatttca atcttcagtt
    34921 ttcctgaaag atacttcccg gtaaggctgt tttcaaaatg cgtaatctcg tcataacttc
    34981 cctggaatat cagttcgccg ccgtgatttc cggccagcgg gccaatatca ataacctggt
    35041 cggcggcttt aatgatctct tcttcatgtt caaccacgat caccgtattc ccgaggtctc
    35101 tcagcccttt aagaacgccg atcagcaggt gtgtgtcccg gggatgcagt ccgatgctgg
    35161 gttcatccag tatatacatg gaaccaacca ggctgctgcc gagagaagtt gccaggttga
    35221 tacgttgtga ttcgcctcct gaaagggtat ttgagagcct gttcagcgtc aggtaaggga
    35281 ggccgacatt attcagaaag cgcaggcgct gttttatttc caccaggatc cggccggcaa
    35341 cgcttttctc atattcagat agttcaaggt tctcaaagaa attcaaggat tctgaaaccg
    35401 gcatcaatac gagctcggta acagatttac cgttgatttt tacaaaattg gcatccttcc
    35461 ttagccgggt tcccctgcat tcggggcaaa ccgttttacc ccggtatcgc gcaagcatta
    35521 ccctgtattg tattttatag gtgttacttt ctaccatcgc aaagaagtcg cggataccgc
    35581 cgaaataccg gttacctgtc cataaaagtt cccgctgctc ttccgaaaga ttatagtagg
    35641 gggtatgcac cgggaaatcg aaatgatggg cgttacggat gagctcatgc ttccattcgc
    35701 tcattttttc gcctttccag caggcaatgg catcatcgta aatcgaaagg cttttatcag
    35761 ggaccacaag gtcttcgtca atgccgataa tactgccaaa tccttcacag gttttacagg
    35821 cgccgtatgg attgttaaaa ctgaaaaggt gcaatgatgg ttcttcgaaa gtaatcccat
    35881 ccagttcgaa cctgtttgaa aactcccttg tattcacgtt cccttcaacg ctgtacctga
    35941 ctgagcatgc gccgttgccc tcataaaatg cagtttgtac cgagtcggca atcctggcag
    36001 gcagatcggc atccgaaaaa tcaatggtaa aacggtctat cagcaactga aggctttctt
    36061 ctgcaaggtt tttcggatta tcaagaatgt cttcgatttt aataatctcc ccgttcgaaa
    36121 cgatacgcga aaatccctgc tgcataatga tggtcagatg ctctttcagg gtacgtccgt
    36181 tcttccttaa caaggggctg aagatcatta caacggctcc ttctgataac gacagcgcga
    36241 aatcaaccac gtcggtgacg gaatcgcgtt tgacaaggtt acccgaaaca ggcgaatagg
    36301 ttttcccgat acgtgcatac aatagtttga tgtactcata tatttcggtt gaggttccta
    36361 ccgtcgaccg gggatttcgg gtattcacct tttgctctat cgcaatggct ggtgcgatgc
    36421 ccctgatata atcaacttcg ggcttgctca tgcgccccat gaattgccgg gcataggcgc
    36481 tcaaactctc gatatagcgg cgttggccgt cggcatacag cgtatcgaaa gccagggatg
    36541 attttcccga tcctgacaaa cccgtaatga cggcgagtgt atttctcggt atggcaaccg
    36601 aaaggttttt caggttatta acccgggcac ctttaatgat gatgtacttt cggggatcaa
    36661 gttgatctat atcggagtat tccattgaaa cgggtacaag agaaataata ccggcaagtt
    36721 gcgcgttcct gaaaaaaact ttgcggtatt tatatcacat ttttaaacaa aacctattaa
    36781 ttatcagggt tgtaaaagta gttttcaaca aggtccggtt aaaaaatttt gaaatggact
    36841 taagtttttg aaaatattgc tttatatttg ccctgccgga acaaaattac taacaattcc
    36901 cttccgaaaa ataaaaagtt aatcaacaca gaatcaaatt aataggagat aaactatcga
    36961 cagatctata cgcttttaaa ctcacataag tactcaacta attcaaagag gaatcatgga
    37021 aacccctttc attagtgacc aggagttgat aggcgaatat ctgtcaggca atcatgccag
    37081 tttagagatt ctgatcaacc ggcacaaaca gaaagtttac tcgtacatct tacttgtcgt
    37141 aaaagacagg tcgcttgctg atgatatatt ccaggatgcc tttatcaagg tcattaatac
    37201 cttgcggacc ggcacctata aggaagaagg caagttcatc caatgggtga tgcgtatctc
    37261 gcacaacctg atcatcgatc attacaggaa atcgaaacga attcccacgg tggaaaatac
    37321 cggcgaggac ggtgatattt tcgatcacct ctatattgcc gatgattccg tggaagacag
    37381 gatgatcatg gaccagatac accaggatgt ccgcagtctc attgaatacc tgcccgaaga
    37441 gcagcgtgaa gtgctcatga tgcggcatta tgccgatatg agtttcaagg aaatcgctga
    37501 acaaacggac gtcagtatca acacggctct tgggcgtatg agatatgccc tgatcaacct
    37561 gcggaaactc attgaaaaga aagaacttat cctgacaaca taaattttat ggttccataa
    37621 aatatcgggg aacgacttcc gttataacac cataaaggaa gttaaacaca accccgtttt
    37681 atggtaaaaa catctacaat tgaaacagtt atttcgaaac ataaggtagc cgggaaggcg
    37741 cacagagcgt cagcaaccat gccttcggat gaatcagttc agtttttgct cagttattca
    37801 agggcgctgc aggttttcgg aaataacatt acaggtcagg cttatgtgat cctgaattaa
    37861 aaagtgttga caattgaact tccccgacgt gcagccgggg attttttttg ccccttaacg
    37921 gctaatcggc tatttttaca tttcaaattc ttttatgtcg gataattaca ccacgaaaga
    37981 gagataccgg cggtttattg aatatttctc cgaacatatg ccggtggccg aaaccgagct
    38041 gcattacgct gacccatatc aattgttggt tgcggtgatc ttgtcggccc aatgcaccga
    38101 taagcgtgta aatatgctca ccccggactt tttcaaaaaa ttcccggatg cagggaccct
    38161 tgccctggcg tccaccgaag atgtttttga actgataaaa agttgttcgt atcctaacaa
    38221 caagaccaaa aaccttattg gcatggcccg gtctctgacg gaaaatttca aaggaattgt
    38281 gccgtccgat attgaggagt tgcagaaact gccgggagtc ggacggaaaa cagccaatgt
    38341 tgtggcatcg gtggtattca ataaacctgc catggcagta gacacacatg ttttcagggt
    38401 ggcagcccgc atcggtttga cgaaaaatgc caaaacgcca ttggctgctg aaatgcagct
    38461 tgtaaaacat attccggaga atttaattcc taaatcgcat cactggctga tccttcacgg
    38521 gcgatatgtc tgtaacgccc gaaaacctaa atgcggggag tgcggactaa atggattttg
    38581 taagtatttt gagacgtttg gggcctccct gcaggacaaa tgacggtcgg cgcctgagta
    38641 ggcacaatac aacgattgat ccggcaatgc aaatggaccg gcagcaatga acagaatggc
    38701 cataatattg ttatcttcgt attaatattt aatgatttta taatgacaca cttaaaagca
    38761 ggcgacaaag ccccggtttt ctccggtacc gatcaggcag gcaaccctat tgattccggc
    38821 tctttcgccg gtaaaaagat agtcctttac ttttatccaa aagataatac ccccggttgt
    38881 accgctgaag cttgcaacct tcgcgataac cacgaggatt tattgcagaa aggctataca
    38941 gtgattggcg ttagcccgga taaccttcaa tcgcacctga aattttcaga aaaatatcag
    39001 ttgcccttcc cccttattcc tgatccggat aaaaagatta tcagtgcatt cggcgtctgg
    39061 gggccgaaaa aatttatggg aaaaagtttt gacggggtac accggacaac ctttgttatt
    39121 gatgaatatg gcttcattga aaagattatc gaacaggtta atacaaaaaa tcatagttcg
    39181 cagataattt gattttcctg aacataaagg aattgaaatg gcatatactt cccggattct
    39241 tagagtatta tccggtttgt tgataaaaaa gaataaaaca ggaaaaggct aaggttcttg
    39301 ttgatccaat ctgcctatat ttgtatgaat ccatttttaa aatcagattt cgatgaataa
    39361 agacgaaaag aacgaaaagc tcagcgccct taaactcacc ttagataaac tcgaaaaaac
    39421 atacggcaaa ggcactgtca tgaaattagg cgatagtgct gttgaagaaa ttccggcaat
    39481 ttcaacaggt tctatcgggc tcgacatagc ccttggtgtg ggaggcctgc ccaaaggccg
    39541 cgtcgttgaa atatacgggc ctgaatcttc gggtaaaacc accctggcgc tgcatgtcat
    39601 tgcagaatcg cagaaaacgg gcggtattgc agcttttatt gacgccgaac atgctttcga
    39661 ccgtttctac gcccagaaac ttggcgtgga tatcgaaaac ctcctggttt cgcaacctga
    39721 taacggtgaa caggcgctgg aaatcgccga taatctgatc cgttctggcg ctatcgatgt
    39781 tattgtgatc gattctgtgg ccgccctaac gcccaaaagc gagatagaag gggagatggg
    39841 cgattcaaaa atgggattgc aggccaggct gatgtcgcag gcattgcgca agctgaccag
    39901 caccatcagc aaaacaggtt gctgctgcat ctttataaac cagttgcgtg aaaaaatcgg
    39961 catcatgttt gggaaccctg aaacaacaac gggaggaaac gcgctgaagt tttatgcctc
    40021 tgtgcgcctc gatatccgta aacaaacgca gatcaaggac ggtgatagcg cgataggtaa
    40081 ccgtgtaaag gtgaaagttg taaaaaacaa ggtcgcgcct cccttccgct ttgccgaatt
    40141 cgacatcatt ttcggtgaag gcatctcacg ctatggtgaa ctggttgacc ttgccgtaga
    40201 gaagaatatc attaagaaga gcggatcctg gttcagctat ggcgatacca aattaggaca
    40261 aggacgcgat gccattaaaa aactgctggc cgacaatccg gaattatcag aagaagttga
    40321 gaaactggtc agggaatctt ataaaaccaa ctaacccgga tcaacaactt aacaagcttt
    40381 gactcctttt ttcatttttg aaaagaggag ttcttatttt tgcactttca aaacaaatga
    40441 atatgcgcca tatcctaaca ttcagctccc tgttgataat tcttactttc accctctcgt
    40501 gcaagcacgc tgggaattca gatgccgaca acaaagggtc tgatcctgtg ctcctggatc
    40561 tgaatgctaa aattgaaaaa gaaccgaaga atgctgacct gtatcagcaa cgggcagtct
    40621 attttatgca gcatgaaaaa ttaaacgatg ccctgactga tatcgataaa gccattgaga
    40681 tcaacccgaa aaatcctgca tattttttga gcctgtcgga tatctatatt atgatgggca
    40741 aaccccagca aggccttgaa tcgattgaga aggcaataac gcttaatgat aaaaatgtgg
    40801 aagcatatct caggaaagca aggttgtaca tgatcatgaa agattatgaa cattgtgccg
    40861 aaagtgttga aaaagtgttc agcattgatc cgcagaatgc cgatgccttc tacctgaaag
    40921 ggtatgtgct gggagaatat ggcgatacca ccaaggcgat cgatgcctat cgcaaggctg
    40981 tgcaatccaa tcagttacat tatgatgccc tcatgcaact cgggtctata ttcgtgaaaa
    41041 aagaccctgt aatggccatc ggatatttcg aaaatgcgct gaaggcaaaa ccgaaatcca
    41101 tggaagcgct ttataatctt gccatgatgt accaggaaaa tgataaacct gatcaagcta
    41161 tcaggctata tggagatatg ctgaaaatag atcccaaaaa cagatttgcg ttatataatt
    41221 cagggtatgt ttatctggta tacctgaata attacactaa ggcaattgat tacttctcaa
    41281 gagcctttca agccgattcc acttatgctg atgccctgtt caacagggga tatgcgtatg
    41341 aactttcggg cgatttcaca agagcaagga cggattacaa taaagtgctt aaaataagtg
    41401 ccaattaccc gaaagccatc gaaggtttaa accgcctcga tactcatggc cggtaaatgc
    41461 attattttgc ttttctttcc tttgataatg cttttatctc ttccctaaat ctaccggcat
    41521 cgcagttgct gcaaaggcta ttgcacgaat taccggtaat gcttttgcct ttctttatta
    41581 aagaccggta tagggctaat aatgtataac cggcagaagc agccagaatc agataggtaa
    41641 ttattgtctg gtattccatc atgcggaagt tactgtgcca tctgaataat aaacatcccg
    41701 gataacgctt ttgttcggta agaaattccg gattccattt cagcccgggc atcacaggtt
    41761 actcaataac ccgtttaaag gaggaaaata gtgtttaacg gagagaaaaa ccataaaagc
    41821 cgaaatggcg acaaagataa acgcccacac catggcagga atgccggtaa tcttatgaag
    41881 atttgtcgca tcgccggctt tcttcttatt gttgtacgta atggccaaca atatcagcga
    41941 cgagattaat gaatccgtta acacgatcag cgagaaaaat gcagcggcta tataaatagc
    42001 cgtctggtta tcgaaataaa tcaggaggat attaagcaag gcaaatgatc cggcccaaca
    42061 gaatccgtat gtatttctga tggataaaat cataagaatc atcacaatgc tcagcatgac
    42121 gaacaggata tatatagctt cctggttttg tatcaggtac atgagcatat atcccgcgat
    42181 ggccgaaacc ggatatccgg caaaagcgat cagaaattgc gaaaacctgc tttttgattt
    42241 ggtggttata gaaccggaag tgtcggcaaa gaggtttata gaaatgacct gtccgctcac
    42301 aatcagggta atgaaagcat gtccggcttc gtgtatcatg gtatttacaa tgcggaaata
    42361 cttgcctatt accggtatgc gggtcagcac cagggcgatt gccagcaata tatagaacag
    42421 taacgttctg ttatttaaaa agctgctcag aatatccttc atgttcagat gatcaaactc
    42481 ccaatccggg aaatcactaa cgaaacaaag taagctaaaa ttgttgtata agtgataata
    42541 aacaaggccc atttccagct tcccgattca cggctgatcg ctgtcacaac accgatgcat
    42601 ggaaaataga tcagtatgaa aatcataaaa gtcaacgcaa ccaaaggagt aaacaccggc
    42661 gagccttcct tttctccgtt catatatacc tgacttctta atttggctat caatgattga
    42721 tcgttgttat tactgtcccc ggtctgataa agtatgccta aagaaccgac cactacttcc
    42781 ttagccgaaa tgcccgtaag cagggcaacg ctcattttcc aatcaaatcc cagggggcgc
    42841 ataacaggtt caataaatcg tccaagcttc ccgatgtaag agttttgttg ctgttcggct
    42901 ttcatgaccg aaaggatggt ctttttctcg gatgtttctt gtttgctcag cttattaata
    42961 gcctgaatgt ttccgtccat cgattctttt aacccgtcat attttgcagt gatcaaaccg
    43021 agctgtctgt tgtaataatc ctgattttta accttttgcg ggaaatagcc cagggcccat
    43081 ataataatgg atgccaccat aatgactccg gcaatcttgc gaaggtattg cccggccctg
    43141 tgccacatat gcttgatgat gctgcggaaa gttggcaggc ggtagggcgg aagttccatg
    43201 acaaaaggaa catccttctg ccggaagatc gttttcctga aaagcagagc cgaaaaaact
    43261 gcaagcatga ttcccaggcc ataaattgca aaaagaattg aaccctggta tcgggtgaag
    43321 aaggcgctga taaaaaggat atacaccgga agccgggcgc tgcacgacat gaatggattg
    43381 atgagcattg tgataagccg gtcccgtttg ctttcgatga tccttgtaga gagaactgcc
    43441 ggcacattgc atccgaagcc catcaccaaa ggaatgaatg attttccgtg aaggccaatc
    43501 ttatgcatta ccttatccat gataaatacg gcacgggcca tataaccggt atcttccatt
    43561 aatgagatga aaaggtacaa caatacaatg tttggcagga agatcaatac gccaccaact
    43621 cctccgatga tgccctgggt cagcagatct ttcagcattc ccggcggcat cgccgtttgc
    43681 acagccgacg atatccattc cacgccgact tcgatccagt tcatcggata ttggcctatc
    43741 ttgaaagtgc cgtaaaaggt agcccacatg ataaatataa agatcgggat gccccagaat
    43801 ttatgggtga taattgtatc aatcagatca ctcttgcttc gtctgtgttc cggattttcc
    43861 ctgaaagtct ctgccatggc gccggcaata aaaccatatt tggcatcggt aatcaggctt
    43921 tcggtatcct ctccgaagtc aagttccagt ttcgaaactt cccgctggct cgtttgaata
    43981 atctcctcac ggttgctgaa agatgaaata taatccctga tagactggtc tttctcgagc
    44041 agtttaatgg ataaaaacct tgaagaaact ttgtcgagga actcccggtt gccgggaatc
    44101 caaatcttat cctgaattgc cgagattgct ctttcaatcg aatttccgta attaatatgg
    44161 atgtgccgtg aaaatttatc gagcccttca tgcacttcaa tgatcttgag aaacagatct
    44221 tcaattcctt tgccccgtga cgcgacggtg ggaatgaaag gaattccgag catgttgcca
    44281 agatgaacat aatcaagttt attaccgctt tgctgcaact catcgaacat attcaatgct
    44341 acgataagcc tgatatccat atcgatcaac tgtgtcgtaa ggaataaatt ccgttccagg
    44401 tttgaggcat caatcacatt tacgactaca tccggcattt tttcgtaaat aaaatcacgg
    44461 acgaaaagtt cttccgggct gtatgcggta atggaatatg tccccggcag gtcggtgata
    44521 acaaactcat aatccttaaa tctgaacctg ctttcgcggg cgtcaacggt aacgccggaa
    44581 taattgccaa ccctgtcgtt tgcaccggat gcgtagttat agagtgtcgt cttacccgaa
    44641 ttgggattgc ctaccagggc aacatggatt gtttttccgg tgagttgctc cggaaggggc
    44701 tcatccgtag cagaaataaa agtgccggaa taagcagccc ggggaatcga tgcgtcatgg
    44761 ctgacttcta tcagttttgc ctcactgttc cggagcgaga cattataccc catgatattg
    44821 tattccgtcg gatcctgtaa aggcgccttt tttataactg tgacctcttt gccgacaaca
    44881 aagcccatct ctgtaatgcg ctttctgaat gccccgcggc ctttaacttt ttgtatgaaa
    44941 gccgtatccc caacatttaa ttcaaacagt gtcactccga aattcttatt tagatcaaat
    45001 aaacattaca aaattaacaa ttcttcagta tacacagatt aattatcaga aaaaaaatgg
    45061 catcaaggaa ttaccaatca aattccttgt tttcagcgac ttccggatat cttcgtgaat
    45121 gtaaaacctg cagggccagt aaaattctaa acccccagtg ggaatagaac aaccttttaa
    45181 tttcaaaaac agcattatca aaagcaagtt gggttggctt ggtaagcaaa aacgcaaagt
    45241 ctttcatgtc ctggtacaca tctgtcagaa tttcggcaag gctcccggta acaatatctt
    45301 cgggatggtc aatgttttct atatatgaaa aagaatcctg atccgccagt tttgcccgga
    45361 tcgaattaaa gatattttcc cattcttctt ccgtaacaaa acgctcattt ccggcgttat
    45421 cgggttcatc cgtctcgggg aacaacaagc ctttggtata gagcagggaa cagagcttat
    45481 gaatcaggga aagaaaatca tttttatcga tgttaccgat gttttccagg gtccgaaaat
    45541 actctgttgc aagagttaat aattcagcgg cctgcctttc cagggccgtt ttttcatcat
    45601 tttcattcat ggtataagat gtggaaaatg taaaagtaca aaagagctct gattccggaa
    45661 ggccgcagcc caatttcccg aaaatgaaaa aggccgcctg aacgacagcc tcctccttat
    45721 gattcggtac aaaaggatta catgcgtttt tccttaatac gtgctttctt acctttcagt
    45781 ccacggaggt agaagatccg ggccctgcgg acagcgcctc ttttgttaac ttcaatttta
    45841 tcgatgttgg gtgaacatac ggggaaaatt ctttctacgc caaccgtgcc cgaaacttta
    45901 cgaacagtaa atgtttcatt taattcatga cccgaacgtt gcaggacaac tccctgatat
    45961 tgctggatac gttctttatc gccttccttg attttgtagt gaaccgtaac agtgtcaccg
    46021 gctttgaaag cgggatggct cgtttcatgg aggaagtgtt tttcaacgta tgtaattaat
    46081 tccttcttgc tcattatttt acgtatttaa taggcgggtt cgtcaaaaat gagtgcaaaa
    46141 atacggcttt ttttcaatac caaacaaatt cgcttgttaa ttttctacag gcttttgtta
    46201 ataactcagg ctggttcagt acggaactgg tttttcagga ataagtactt ttgcagcgaa
    46261 ctgattcaat aggttgatgc ccgaaaaatc tctatttgct taataatgac ccgattatgg
    46321 atttgtcatt catcctgaat aaccttggcg aagatatagg aaacggctct tcaccggtaa
    46381 cccctcccat ttatcagaca agcaattttt atttcaaaac agttgaggac ttcaggcagg
    46441 cgattctgtc agagcgcgac cacctgatat acaccagggg taataatcca accatcgatt
    46501 tattacgtat caaaatggca gcccttgaag gtgccgaaga tgcccttgta tttgcaagcg
    46561 gaatggccgc aatttcatct gccatcctgg cgatggtgaa aaccggggat catgtgatca
    46621 gcgttcagca tccctattca tggaccgata ccctgatgaa ttcaaacatc ttcccgaaat
    46681 tcggcattga agcaacaatg gtggatggta cggatgcaag aaatattgag caggctataa
    46741 aaccgaatac cagtctcatt tatcttgaaa gtcccaattc atggaccttc gatatacaga
    46801 atctgggtga aatcggtgtc ttggctaaaa agcacggtat catcaccatc atcgacaata
    46861 gttacagtac cccgctgtac cagcaaccct taaaaaatgg cattgacctg gttgtgcata
    46921 cggccagtaa atatcttggc ggacatagtg atgtggttgc cggcatttgt tgcggttcca
    46981 gggaattgat cgacaaaata ttccacaacg agttttgtac gctgggagga atcatctcgc
    47041 ctttcaatgc atggttgctc ctgcgcggat tacgcaccct gcaggttcgt atggaacata
    47101 tcagcagaac ggcaggggag gtgatcgcct ttctgcaaga cagcggcatt gttgaaaaaa
    47161 tttactatcc ttttcttgaa gggaatcctg ggatgcaaac agctaaaaat cagatgacta
    47221 aggcatccgg gctatttagc atcgttttaa aaaccaggag tactgctgct atcgaagaat
    47281 tttgcaacag cttgacttac tggcgtatgg cggtgtcatg gggcggtttt gaatcgctta
    47341 tcattccttc atgtactttt gtcagacccg gcttatattc aaatttgccc gataatatga
    47401 tacgattttc ggtgggcctc gaaacggcag acgctctcat cggggatctt gaaaagaacc
    47461 ttcatttgct ccgataaaaa aaacgcaccc tgagcggatg cattttaacg ggaaagtcca
    47521 aagaaagccc ttcttatttt atatccgggg cattttcttc agcagtgtcc ttagcttcgc
    47581 cggtactggt tcctgcgttt cctccctgat catcaggttg tgcctgagca ggagcagcgg
    47641 tttcttttac atcctcggca gaagcttttg aagcgctacg aacttttagc tcaaggtcgt
    47701 ccacgaactt ttcagccttt tcctcaattt tatcgagcgt tccctcggct ttttcaacga
    47761 tgtcgtcttt aatttccttt gctttaaccg tagcttgttg cgctttttcg gcaacgtttt
    47821 ctacaacatc gtcaatttta tcggcggttt tttcttttaa gtcctcaaac ttttcagcac
    47881 ctacctgctt cgaagtttta aatagttttt ttaaagaatc tttaagtccc atggcattaa
    47941 gattttagat ttttgtaaag atataaaaga aatgtgacga tggtattttt ctaggattct
    48001 ttttttgctt ttcacaatga tttattcgct gatgagtccg ggccgtcttt ctttggtcct
    48061 tgttacggcc tgttccatct gccatttccg gatttctttc tcattgcccg agagaagaat
    48121 ttcaggcact ttccagccat ggtaatcggc cggacgggta taaacaggag cagaaagcag
    48181 gttatcctga aatgaatcgc tcaatgccga agtttcatca ttcagtacac ctggaatgag
    48241 cctgataacg gcatctgata taacggctgc tgcaagttca ccgcccgaga ggacataatc
    48301 gccgatggag atttcagaag tgatcaggtg ttcgcgaatc ctttcgtcaa tacccttata
    48361 atggccgcat aacaggataa tattttcttt aagtgacagg ttgttggcta ttttctgatc
    48421 gaattgcttt ccatcaggag tcaggaatat aatctcatca tagttccgcc gctcttttaa
    48481 ggcttcaatg caatcggcaa taggctggat catcataacc atgcctgctt cacctccaaa
    48541 agcatagtca tcaacatttc tgtgcctgct ttttgaataa tcgcggatat tatgtatgtg
    48601 aatttcagcc agaccttttg caatcgctct tttcacgata gaatgatcaa acggtccgga
    48661 aaagatctca ggaaagatgg tgagtatatc tatgcgcatg atgtgacggt tcggttgcaa
    48721 atgtaaataa gattttgcat agcctttttg ctaaaacgtt cataggattg actcaattat
    48781 tatttttgta ataaccaata ttacgtacca tgcgtttgtt taccatcaca ttggccctgc
    48841 ttttagctgc catcagcgtt ttttcgcaat cttcagataa agaaatattt gttgtcacct
    48901 tctcggacaa aggccctgat atactaaaat ctatacttgc acctgaaaat tatctttcgg
    48961 agaaagccat ttcccggagg cagaaacatg cagtttcgtt tgatgaatcg gatttgcctt
    49021 tgtatcagga atatgtgagc caggttcttt caacgggagt taatgtgctt tcacgttcca
    49081 ggtggttaaa ttgcatcgtt gttgaaggga caaaagatgt acaagggaaa cttaacaaga
    49141 ttccgggagt gaaaaatgtt acaattttaa agacttcagg accgcctgat cataagaatt
    49201 tcgataaaaa atcgatagtt gctttaaagg aatataacgt aaaagggttg cagaaaaaag
    49261 ctaacggttc cgatgtttat aattacggag cggcagagaa ccagatatcg atgatcaaag
    49321 gtcagtatct tcataacctg aactattgcg ggcaaggcat gacgattgcc gttcttgata
    49381 acggtttcaa cagggtggat atcttatctg cttttgatag tctatggatt aataatcaaa
    49441 tccttggaac taaagatttc gtaacgccgg gggcaaatgt cttcgatccg gatgccggtt
    49501 cgcacggaac aatggtttta tcgaccatgg catccaacat tccgggaacc atagtaggca
    49561 cagcccccaa agccgactac tggctgatcc atacagaaga caatgcaggc gaatcatata
    49621 tcgaagagta ttattgggta agcggcgccg aatttgccga tagtgttggc gctgatgtga
    49681 tcaattcatc actgggctac accacttttc ccaattcccc ggcatatgac catacctatg
    49741 ccgatatgga tgggaacacc tgcgtttcaa ctatcggagc cgatatggcc gtaaaaaaag
    49801 gaattctggt tgtaaacagc gccggcaact ccggcgaaaa ttacgatccg tggcagtata
    49861 ttggtgcacc ggctgacggt gatagtgttt ttaccatcgg agcggttgat ggaaccggta
    49921 tccgggcttc attcagttct aaaggcccga cttatgacgg tcggattaaa ccggatattg
    49981 cggcgcaggg gcttgatgat gcagtctttt tctgggataa cggaacccag caagggaaaa
    50041 tttattacgg cagcggcacc tctttttcct ctcccattat tgcaggaatg tcagcatgcc
    50101 tttggcaatc agtgcctgat aaaagcaata tggatattat ccagagcctt cgtgccacgg
    50161 caagtaattc tcaaatgccc gatacactgg tcggctgggg gatacccgat tatgaacagg
    50221 cccgtctcca gctttcctta ggaacaccgg aattaccagc caggccgggt ttaaaagtat
    50281 ttcccaatcc attcagcctg tcattcaccg ccatcttacc ggaagccctg gaaggcagga
    50341 ttgtggctga gatcatcagc ccggatggta aaattatgat aagccgccac cttgattcct
    50401 cattcccgat acagtcatta tatatgaatg atctgacagg ccttaaagca gggatttata
    50461 ttttacgtgt tattgataag aacaatgttt tcacttcaac ctgtatcagg tattagtttt
    50521 tgatgtctct tttccatccg gcacctgaaa ggaggccgtg atggttatat tcaaaaacca
    50581 tttcatgtct ttttacttgt tcatggaatc atttcatgaa taaccggttt gtgcttcttt
    50641 ttaaacggag gagaattgaa taattcctgc acctgctaag gatcagtata aattttcgat
    50701 tctcaggaag tatttatgat ttcattttgg actaaaaaaa ttgtcagaca atgtattaac
    50761 ggcttttatt gacgatcgaa tgttgaataa ccaaacagaa ttaagtacgg cgtcgtcctt
    50821 ttaataaaat aggagtgagg tttgaataat aaaatgccag atcgaggtaa tttaaaagat
    50881 tacaaaagta aacccaatga gattacataa ggaaactaat aataactaca aacgtatact
    50941 ggtaattctg aaatgtaaac catgctgaaa ttcaattgta tttacaaatg tattttatca
    51001 atacaagtat acatttatat cctaccctgc aaaaagcctt tatttaatga atgctttgtt
    51061 ataagtaaaa gataaccagc gcatatctta atttatttaa agtaataatg aaaagaacat
    51121 ccttcgcaac ctagcatcgg aaatattttt acatataatt atatttaaat gatatttagt
    51181 ctgttaaaac agtttatttt agtcataaat ttaatattca acaggaaggg ttaacattat
    51241 aaatatttat ttcataagtg aatgagaaat gtgaatctga ttacttacat ttgtagtgta
    51301 ataattttaa tgtatacacc ggtatgaaag aaagaatcca gctattgatt aaggcgaaaa
    51361 atttcacagc cgctcagttt gctgatgaga tcggtgtgca gaaatcaggg gtttctcaca
    51421 tcatatccgg acgcaataat cccagcctgg attttgtaca gaagattctg acgcgtttcc
    51481 cggaagtcag caccgattgg ctattatttg gccgggggcc tatgatgctc agcgatgttc
    51541 cggccacttt ttcattaccg ccggttgctg ttccggcatc ttcttcgggc aagttagcgg
    51601 atttgttttc acaggatatt gctccggttg aggtgtctgt taagcctgtt aaagtcccgg
    51661 aaaaactaat tacagattct gttcctgtat ataagactgc tattgatgaa cctggcgaaa
    51721 aagagtcagg aaaacctcag gaaaacatca ccattcctgt tccaaaggat gttaaaaatc
    51781 aggaacaggc gaaagtggtc actaaaatta tcgttctgta cgatgataag actttctcgg
    51841 aatatcatcc agaagattaa aatctttcga tccgggagaa aaagaaatcg ctttcaatca
    51901 cagcatttgt atcacaatcg gagccatgga cggcgttttg ctgaatggag acgccatata
    51961 actttcggat gctcccttcc ggcgccacca acggatcggt agggccgatt agcatacgga
    52021 aatcgtccac agcattctct ttttccagaa tacatgccac aataggtcct gaagacataa
    52081 attccaccaa tccatcaaaa aatggttttc ctcggtgaac cgaataaaag gtttcagcct
    52141 gcatccggct gatttgtgtg tacttcatag cctttataga aaacccgttc tgagtgaata
    52201 ttgtaaggat agggccgatt ttaccagcgg atgcggcatc cggctttatc atgctaaatg
    52261 taatatttcc agccatttca atcaggattt ggatgagcga aaggtaataa ttttattgca
    52321 ttttcgattg atgcggccaa caattcggga ttaattggta attttgtaac ggattagaag
    52381 taaacccgtt aactattacc cctattgaat ttcccagaac aaacaatttc aaagctgaaa
    52441 gatattctgt ccatgccaca gaatattctg atcacgacac attataatcc tgatggtgat
    52501 gcaattggag catccctggc actctatcac tttttagtga aacaaggtca ccaggtcgat
    52561 ataattgttc ccaacgaact tccatcattt ctaacctgga tgcctggaaa tgaaaagttg
    52621 ctgatttaca agcagaaaga tgatattgcg cacgatataa ttgaaaatgc cggtgtgatt
    52681 ttttgcatcg atttcaatgc cctgaaccgt gtaaaatatt attcggatca gcttgcagca
    52741 gcaaaaggat ttaaaatact gattgatcat catttagagc cttcagatga attcgacctg
    52801 acgatttcgc agatcaaagt atcttcaaca tctgaattgc tgtaccagtt gatacaggaa
    52861 accggttatg gtgattgtgt ggatttgccg gtagccgagt gtatatttgt cggaatcatg
    52921 acagataccg gttcattcag ttattcctgc gaaaaaccgg atactttccg gattacttcg
    52981 gaactcatca ggattggcgt taaagttgaa aaaatccaca ggcttgtgta tgatacctat
    53041 tctgagagcc ggatgcgatt gctgggtcat tgcctgagtc agcgtatgaa agtgattccc
    53101 gggttgagta cggcttatat ctggctcagc aaagaggatt tggaagcctt tgattatcag
    53161 ccgggagata cggaaggggt ggtaaattat gctttaagca tcgaaaatat tgttcttgcc
    53221 gccttgttta ccgaacgcga tgacaggatc agaatctctt tacggtcgaa aggacaattc
    53281 tcggtaaacg attttgcgcg gaaaaatttt atgggagggg ggcaccgcaa tgctgccgga
    53341 ggagattctt ttctcagtct ggaagaaaca atgacaaaat ttgaatccct gcttgaagga
    53401 taccgtgatg agttaatgaa caaccggaaa tcttaaccga agtttttcat gatgaaaaag
    53461 atatatttcc ttgcctttct gttccttttg tctggttgtg gtcaaaaaga gggaaacagg
    53521 cctgagaaat cagatgctgc cgatttaaat gaaacgctta tcaaggcaaa caagcaagtg
    53581 gtactctccg aagaccagca gatagatgat ttactggagc gttacggatg gaatatgaca
    53641 gagaccggta ccggattacg gtatttgatt tataaacgcg gaaacgggcc gtcggctcaa
    53701 aaaggtcaac aggccacgat ccgatataac ctcagactga tttcaggaga tatcgtttat
    53761 tcctcggatg atcttgggcc gaaacaattc actatcggaa gcggtggcgt ggagagcgga
    53821 ctggaagagg caattttgct catgcacctg ggtgatcaag ctaaagttgt cataccttcg
    53881 taccttgctt tcgggctgat cggcgatcag gacagaatac cgccgaaatc gaccttaatc
    53941 tatgatatag aattaataaa attaacaaat aactaattac caatacattc acaactcaac
    54001 ttctcatgaa aaagattctt ttagtgatcc cggtagcctt gctgcttact ctcgtacttt
    54061 cctcgtgcgg taaacatgcc ggttacaaaa aaactgacag tggcctttat tataagttct
    54121 atacacagaa tgaggactca gtaaaggctg atacctctaa gatagccaca ctgctcatca
    54181 gttaccggac tactgtcaac ggaaaagact ctgtgatttt tgattcaaaa accaggaacg
    54241 ttccgtttga aatcaagttg atgaaaccgg tatatcccgg tgatattttt gaagctctca
    54301 gtatgatgca taaaggcgac agcgcctcgt tcatcctgaa tgcaggcgac ttctttctga
    54361 aaacagccca ggcgccccag gttcctgaag gtatagacag cacggcagac atgatattca
    54421 atatcaggat gctaaaagta cagaccatgg aagaatacca gatggataag gcacagcaac
    54481 tgacccggca aaaagacaat gctgcaaaat cattacagga ctatctgaca aaaaacaatg
    54541 ttacaacggc tcctacggct tcaggtgttt atatcattca tcagaaacca gcaacaggtc
    54601 gtgccatcaa gaaaggtgac tttgtcaaaa tccatttttc tgttacgctg atcgatggga
    54661 aaaaactgtt ttccactcat gacaatggta cccctatgac atttgaattt ggacaacctt
    54721 ttgatacaaa aggatttgat gaaggaatcg gggccgtgaa agaaggtgaa acggcacgtt
    54781 tcatcgtacc tggtgaaatg gctttcggtg aacaggggcg tcgttcgatg aatggtgaag
    54841 aactcattgc tccttatgcc actattatct atgatgttga agtactcgat gtaagcacca
    54901 aggcagagca tgaaaaacag gtagcagaag aaactgccgc taagaaaaag gctgccgacc
    54961 ttgccaaaaa caatgaacct tcgctcataa aaaagtacgt ggctgataat aagattactg
    55021 taaaacccac cgcttcaggc ttatactata ttgagagagt gaaaggaaaa gggaaaaagg
    55081 ctgaaaaagg tatgaaagtt cagatgaact ataccggacg tttgctcgac ggtaccaaat
    55141 tcgactccaa cctcgaccgc aaaccgttaa aaccttttga attcacctta ggccagcaag
    55201 aagtgatccc aggttgggat gaaggtgttg ccttgatgaa tgaaggcggg aaagctactt
    55261 ttattattcc ttcgaaatta gcatacggcg aacgtgaaat gggcggcaaa atcaaagcct
    55321 attccccgct cgtatttgaa gtagaacttg ttaaggttct tggtaaataa gtattagaaa
    55381 ctgaaaaaag aaggctgttt cctgtcatgg aaacagcctt cttttttata gaaagagatg
    55441 caccggaatt tacaagactg gttattttct cttttctaaa agatctttat acgagagcat
    55501 ttcatcgcgt aaccgcgcag cctctataaa atcaagttct ttggctgctt tttccattgc
    55561 cctgcgcgtc tggtccagca tcttttttaa ttgttctgtc ggcatatagt gtattacggg
    55621 atcggctgca acgtcaatct tgtcattttc aatgtaggca gcagcctgcg gtttggcttt
    55681 cgcatcagca accgttgtct ggcccataat ctgttcggcg ctttttatta tggcagtggg
    55741 agtgatgtta tgcttctggt tgtaagccat ctgtttttcg cggcggcggg ccgtttcttc
    55801 catcgcccgg cgcatggaat ctgtaatctt atcggcataa aaaatcactt ttgcattcag
    55861 attgcgggcg gcacggccag ccgtctgggt aagggaccgc tccgaccgca ggaaaccttc
    55921 cttatcggca tcaaggattg ctacgaggga tacttccggc aaatcaaggc cttcgcgaag
    55981 caggttgaca ccaaccagta catcaaacgc tcccatacga aggtcacgaa gaatatcaat
    56041 acgatcgagg gtgtcaacat cactgtgaat atactggcaa cggattccaa gtcttgtaag
    56101 gtacttggtc agttcttccg ccattctttt ggtcagggtt gtcaccagcg tgcgttcgcc
    56161 tttctgaatt ctttgctcta cttcgtcaag taaatcatcc atctggttca ggctgggttt
    56221 cacttcaatg agcggatcca gcaaacctgt cggacggata agctgttcca ctacaacgcc
    56281 ttgactttgt tgaagttcat aatcggccgg ggttgcgctg acataaataa cctgcgggta
    56341 catcgattca aattcttcaa atttcagcgg acggttatcc attgctgaag gcagccggaa
    56401 cccgtattcc accagggttt gtttccgtga ccggtcgccg ccgtacatgg ccctgatttg
    56461 cggaaccgta acatggcttt catcaattac cagcaggaaa tcatccggaa aatagtcaag
    56521 aaggcagaaa ggcctggctc ccggcattct tccgtcgaaa tagcgggaat agttttcaat
    56581 tcctgaacaa taaccgagct ccctgagcat ttctacatcg taggtaacac ggtcttcaag
    56641 cctttttgct tccaggttcc tgttgagcga tttaaaatat tcaacctgct ttaccatatc
    56701 cgattgaata tcccagagcg catccttcat tttatctttg gaagtcacaa aaatattggc
    56761 cggaaaaagc acaagaccgg gtacagattc aatcattttt ccgttcaacg gatcaaaaga
    56821 atggatgctt tctatctcgt cgccgaagaa gatcaccctg taggaaacat cagtataagc
    56881 aggaaaaata tcgacggtat cgccttttac cctgaatttg ccgcgggtaa aatcaatttc
    56941 agtcctggag taaagggcat caaccagggc atacaacagt ttattccggc tcaaatgatt
    57001 tcccacttca atccggatta cattctttgt gaaatcatcc gggttaccga taccgtaaat
    57061 acaggacacc gatgacacga cgatgacatc acggcggccc gacatcaacg acgatgacgc
    57121 gcttagcctg agcttttcta tttcctcatt gatggaaagg tctttctcaa tataggtatt
    57181 tgtgacgggt atgaaagctt cgggctggta gtaatcgtaa taggagacga aatattcgac
    57241 ggcattttcg gggaaaaact gcttgaattc cccgtacagt tgggctgcaa gggtcttatt
    57301 atggcttaaa ataagtgtag gacgatttgt ttcattgatg acattggcaa tcgtaaatgt
    57361 ttttcctgat cccgtgacgc caagaagtac ctgtgaggca tcgccttcat tcaatccggc
    57421 aaccagttgt cggattgctt ccggttgatc gccggttgga acatattccg atgttagctt
    57481 gaaattcata tgaagcttct cttttgtaaa accggcacta atccggatgc gaaccgggtt
    57541 ttatgcgggc catgataggg tagtggtcag aatatggttt tccgatcaca taaaaatcat
    57601 tcactgaaat ggatttgctg gtaagaatat agtctattct caggaaagga agcacttcgg
    57661 cataagtttg accgattcca tttccagcca tcacaaatgc atcattcagg ctccgggtca
    57721 gtttttgata tgcataggat acaggagtgt cattaatatc cccgcaaaaa acaacagggt
    57781 aaggagaaga atttatgatt tcctccaggg ccggtatttg ttttgaccgt cgtgcaaatg
    57841 ctgattttat ctttcgcagg ataatccttg aacctttgtt gtaatcctta ttcggatcat
    57901 tcagcgaatt ccattcagtt acaaggctga aatcctcttt gcctaatcga acggattcca
    57961 ggtgggcatt caatacccgg atggtatcgc tatgcactat aatatcgttg acaatgcaaa
    58021 agttcacatc gctgttttca aataaaatct tatgtgaatg aaggatagga tatttgctga
    58081 atgtggccag acctaccggc aggtcctttt tttccgactc aataaatgca gcaaagccat
    58141 atttcgtatt gatcgctttc gtgatggtat cggcataatc ggcttttagc cctttgccgg
    58201 aataatactc ctgaatacag agaatatcag gtgattcggt tttgatcagg tcaaaaatcg
    58261 ccttacgcat ttccctatgg gtgtttcctt tccatccata cagattaaac aacctgacat
    58321 tatacgacat cactttaata tcttctgaat caccggtttt atcatgatgg ttgccgatac
    58381 tgaaaaagga tatcaggtaa ctgtatccca acaggatagc gatcagggaa atcaggaaat
    58441 atcttttaag gtaaacaagc cagaaaagaa tgaagaatat attaatgatc agaatgaccg
    58501 ggtaaaataa accgaaaaat gcaagaggcc agaatgtttc aggagatata taccttgaca
    58561 gatatgccag cagcagaaac aaaacaacga tccagttcag aaagagggtt aacccagttc
    58621 caaacgaacg cttttttttg ttcgatttct gtgattccaa atgcatgaat ctgcgaactt
    58681 tttgtgctta cagggctgat taattatttt ctacttgttt taaacagaaa ttctttttct
    58741 tctttcgaca atgttgaata acccgatttg gctattttat ccagtatctc atctattttt
    58801 ttctggtcgg tagctcttcg tgcattgtat tcttcgtcgc tcaagggacg acctgaagta
    58861 tgaaccttcc ggaattttac ctttttcatc cgggccgttc ctctgtgttg accctgaatg
    58921 cgtttgaatt ccaaaagcct ggcaggatca atgttttttc gcaacaacag catataggtg
    58981 aatccccaca aagcgcctcc caggtgggca aaatgtccgc cggcgttacc cgaacggatc
    59041 atcaggatat ccgtcacgat catggctatt gcaatgtatt tgattttgac ctgacctatc
    59101 agaaacagat tcacggtata atcgggcacc ataaacgaaa tcataatcac gatggccagt
    59161 actgatgctg atgctcctag tgccaatgcc tggcccagac tctgctgaaa aacggggaaa
    59221 aaattaaaag ctgccataaa cacgatgccc ccggcgattc cccccaa
//
LOCUS       DAOPYV010000008        56016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_429_length_56016_cov_12.789907, whole genome shotgun sequence.
ACCESSION   DAOPYV010000008 DAOPYV010000000
VERSION     DAOPYV010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 56016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56016
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_429_length_56016_cov_12.789907"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2613
                     /locus_tag="PKI35_01815"
     CDS             <1..2613
                     /locus_tag="PKI35_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNX43143.1"
                     /translation="LTNLSTGPSNWRYVKADWGVNIPDCKLIPLGNDKWQLNIGPTIR
                     DYYGVPSAEQILQLAFVFRSGTKVNGSYLEGKTASGGDIFYDVYPAALSIKITSPDED
                     LVFVQLNETFTVKVASSFADSTFLFVDGTRVAATTADSLVYNITPDAYSRVLVKARAV
                     NDTAVVADSFYYYVRGPVPVAELPEGVTEGINYIDSTSAVLCLYAPMKEYAFVIGDFN
                     NWLPDELSYMKQTPDGNRFWIQIDGLVPGKEYIFQYLVDGNIRIGDPYAEKVSDPWND
                     SYIDDSTYPDMLAYPTGKTTGIATVLQTQKPGYEWKNISFTPPLATDLVIYELLIRDF
                     TSQHTFQSVIDTLTYLKQLGINAIEFMPVSEFEGNSSWGYNPNYYCAVDKYYGPENKF
                     RELIDSCHSNGIAVIMDVVFNHAFGTSPYVMLYWDKANNRPATNSPFYNPVAKHPYNV
                     GYDMNHESADSKYYISRVMRHWLTRYNVDGFRFDLSKGFTQNYTTDVGIWGHYDASRI
                     SILTAYHDTLQAVKPEAYMILEHFADNDEESVLAARGMMLWGNYSGQYGSAAAGSVAA
                     GNFPGISYVSHGWFKPNLVGYMESHDEERLMYRCISEGYVSSAYNIRDTVTALKRAGL
                     AATFFFTVPGPKMVWQFGEQGYDYSINWPSGSVNDRLTPKPPRWDYPDQHNRLLLLYD
                     YISLIKLKTTNPLFRTTDFSLDVSGAMKKIKLRNEEMQAVVLGNFDVKQGNISPGFYH
                     TGTWYDYMTGDSIVVSNADSAITLAAGEYKLYLSKRINNPWGISGKQVADVTVDIAPN
                     PVIEQTTIRVAAPGMKRCIIRSFDMTGRFTGTLFNGTFCNQMEITWQPLHKGLNILVI
                     EAGSAVTIKKVLVL"
     gene            complement(2688..3161)
                     /locus_tag="PKI35_01820"
     CDS             complement(2688..3161)
                     /locus_tag="PKI35_01820"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43144.1"
                     /translation="MKRMRTLPFFLLFSLLLACKPGGKYELKNTFTNDTWDRFNKLLF
                     EMPKELKPGSYDISLVLKHTDGLECDEFPVYVILDTPSGEERMNEVPLKIRDKEKFCG
                     ASKDGYYTITQKLWEGIEISDTGACTISVENLNPKFQTPGIHEVAVIMEKKSESR"
     gene            complement(3190..4254)
                     /gene="mnmA"
                     /locus_tag="PKI35_01825"
     CDS             complement(3190..4254)
                     /gene="mnmA"
                     /locus_tag="PKI35_01825"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005680511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNX43145.1"
                     /translation="MKIAALVSGGVDSSVMLHLLKEQGFEPTIFYIRIGMKEDEHFLD
                     CSSEEDIELTSWLANHYGLKMEVVPLHKEYWDNVMAYALDAVKKGLTPNPDVMCNRLI
                     KFGEFEKQWGRDFDKIATGHYATTIESDGVTWLGTAIDPVKDQTDFLAQITYEQLSKL
                     MFPVGNMMKSEVRAIARSQHMPSAHRRDSQGICFLGKVNYVDFLRRYIGEKEGPIIEL
                     ETGKILGKHKGFWFHTIGQRKGLGLSQGPWFVVKKDTDQNIIYVSNGYDPLAQYGSEI
                     RLADFHFITKNPWPGLIQPQRITFKIRHTPDFTAGTLQQDGRHLVIKSSVPVSGIACG
                     QFGVVYDEEHRICLGSGVID"
     gene            complement(4337..5299)
                     /locus_tag="PKI35_01830"
     CDS             complement(4337..5299)
                     /locus_tag="PKI35_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNX43146.1"
                     /translation="MKNILVIGAVGQIGSELTLELRKRYGNSNVVAGFRATKPTGELL
                     ESGPAEVADATNAQQVADVVKKYKIDTIYNLAAILSAVGESKPLMAWHVGIGGLMNCL
                     EVARENGCAVFTPSSIGAFGGGTPLDNTPQDTIQRPQTMYGITKVTGELLSDYYFRKF
                     GVDTRSVRFPGIISNVTLPGGGTTDYAVEIYYEAVKTGKYTCPLKAGTFLDMMYMPDA
                     LNAAIDLMEADPSKLKHRNSFNVTAMSFDPEIISAEIKKHIPAFAMEYQVEPVKQSIA
                     NSWPNKMDDSCAREEWGWTPEWNLEKMTIDMLKAIREKKAKGLI"
     gene            5575..6765
                     /gene="kbl"
                     /locus_tag="PKI35_01835"
     CDS             5575..6765
                     /gene="kbl"
                     /locus_tag="PKI35_01835"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNX43147.1"
                     /translation="MYENFQSFLQKELTGIEEAGLFKNERIIVSPQGAEIKINTGATV
                     LNFCANNYLGLSNNPKLIAAAKKALDSHGYGMSSVRFICGTSDLHKELERKISEFFGT
                     EDTILYAACFDANGGVFEPLLGEQDAIISDSLNHASIIDGVRLCKAVRYRYANADMAD
                     LEKQLQAAQAQRFRLVVTDGVFSMDGNVAPLDKIYELANKYDAMVMVDESHSAGVVGR
                     TGRGVTEQFNLRGKIEILTGTLGKSFGGAIGGFTTGKKEVIAMLRQRSRPYLFSNSVP
                     PLVAAAGIRMVEMMSETNELQDKLHENTAYFVKKMMAAGFDIKPTQSAICAVMLYDAK
                     LSQEMAAKLLDEGIYVTGFYYPVVPKGQARIRVQISAAHEHAHLDKCVAAFTKVGKEL
                     GVLK"
     gene            6959..10969
                     /locus_tag="PKI35_01840"
     CDS             6959..10969
                     /locus_tag="PKI35_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNX43148.1"
                     /translation="MKRILPFLLFLGVFSFAISYGSLAQKSGSTPGAAGVQAANANFS
                     RLPLAPSIYWAPTSLVDTVWTGLDNNQPLYLINNGDTALNFMFPNYFEPNPVVSPYTY
                     CSASGGCDEFISTVAINSITNSSGCTGYGNYTSVYTHLNTGMSYPVTVTLGPPSYSQD
                     VTGVWIDFDHSSTFDEDELTQLSGYLPATGVITIPADASLGVTTMRIRTQYGGTLSPC
                     GTTDYGEVEDYTVCIRKPSFVTDIYPVSGMIEPGDTVSVNITTSATGNYAAYGVYYDT
                     LLLSTNDTVNPAINIPAEMHVLGYGELQGSLIDYVTHVPLPGIMVKALQNITMTDDSG
                     HYSLSLMEGPYEVKFTRPGYDTIVAPLTIIRDTTVIYNDSLHFYPYRPACAYAEVING
                     DSACQITWCKPAGPQLLLYDDANAENYFTWDSICRINAVRFTPDDYPATLTGGQFYLG
                     SIPYDGLPFTVAVYDDDGAGGLPGTLLDSVSCTTVFAPDGWITVNGLNATISSGDYYL
                     SILHQCDSASCITLGVDETDPDAGRSYTRNMTAGEPWMLSTVQDFMIHAIVDEPVPPY
                     SGKALARNSGSGMTDGLKSSGNDDASPAGNNDALEGLSHYMLCRAPVIDPLAGPQYGT
                     DILLANDLIDTSFLEGGTTWICLPAGWYAYGIRGIYSDGSASEWRFTEPVAHKLYADV
                     SVTVLSECNTMPLQGATVTLAGGEYPFDTLVQQTDTGGIASFSDIIKGVYEMSITSPG
                     FETYNVFYNIQSHSTMEIILQVNPAKPENLSVDGATLLATWEPPQFDPPGSIADTSVL
                     QGYAVYLDGTLQGQTQELYWDFSPACLNTGQSYTAAVAGVYCTGIGEADTFIFTSSYL
                     PPPQNLTASATASVGIGTATFSWQMPDTSCTACDPVSFMIYNFGNLIGEIPAENLSYT
                     IPSASPTTECLAVSAKYDLSCMGFPGQFGESAITEEVCIDITLGNDIPFSEDWGSGLF
                     ETNLWTPGQNWVIAGTGDSPSPAARFVSEPNLSGYSSALQSHWINALLTDTVMPYDIY
                     LYYDLKLQKVAASSTEKLAVEVFGADGWTRVKEYVNSENLDLAQERLLISQQAKNRIF
                     MIRFVAEGENTSNISWWETDNISVKAELTGVAPANLTASPYGNDGDDILLQWQPPAAG
                     SYAAGRQGNNAGFFEPGSSEEVATIAPAELLGYNIYRRAFYSPNPGPGPGSGLWSLLA
                     STVSGDTLYIDENLPNLETNCYEYYVTSFFSQGESSSSNTDSECICVGMKENTAEVPL
                     LYPNPSDKWVNIILPERCKTLSLFNIAGKELKKIDTNGKQEITLDIKGFPSGAYLVRF
                     GLSDGERITQKLLIRH"
     gene            11125..11931
                     /locus_tag="PKI35_01845"
     CDS             11125..11931
                     /locus_tag="PKI35_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43149.1"
                     /translation="MKTSIPLLAVFAFMAMASFYSCEKSSDSGDVTKDIIINKTSDSV
                     LIFGSNSDSLILTPWDLVLQGYYHTDESAELDIDGDSIADIRLVSSIEGLAGTAPIPA
                     SYIKCLHSNVMLRNRHKSDTITMKETCDTVYDNGRMIIQTVRTFECNTYLPGDSITEV
                     REINHIVYNIGGDSVATYDLFESGSYTLASSPYTTPWMTGFQSDSLVITSMGVYNDNC
                     CTMPDDVPVYIGFKIITDGKPRLGWIKLSVHDYSIIHIYETAIQPVSAVK"
     gene            complement(11951..12082)
                     /locus_tag="PKI35_01850"
     CDS             complement(11951..12082)
                     /locus_tag="PKI35_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43150.1"
                     /translation="MESLDSLNNEYSSARAAFPKQEINPLSPPTEVFKGIDFRLTEA"
     gene            12112..14118
                     /locus_tag="PKI35_01855"
     CDS             12112..14118
                     /locus_tag="PKI35_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43151.1"
                     /translation="MRTLIIKLFSVAVILMAVNNRISAQQQDMAWPGPKGVFIFTGDK
                     IPTGQVIYAYQVERTGPDSVWVKIGVLRTPATFQQFLDKVNDTKKIFPDQPLPASAKL
                     EQLFKKALATGTVDSLTGWKNYPVRMALGVMYYDDHAVNGKFRYRISEVDTARKVVSS
                     VITNAVSMPHTAKFDPIMVDESSRTAHLNYIRWYSAGNNPAPLFQPFRFENGKNIAIS
                     GWTSRNSVNDTSYYTFTDSVPGAIKAEQFFIVPFDPFGNAGAASEILKTGPDRFTNSK
                     INKVKAGRIPDKYGIRVSWFYNAPKQIKNFIIYRSENKDKGFREYTRVPSADTVYCDV
                     NAWPGHTYYYYLKAYDTTGKRFITSEKAWYTASLASKPGAPKVTAEAVKGGVKLTVDV
                     NDDKADGVRIFRNDGLSEQMIAITQLIPLNGKQTIEYTDSSENLSGRHNYCYQVRSEA
                     KDAGVSELSEKATAKPLINTPPADPAFIRGSVADNKINLLWQEAGSADSSITGYKLTR
                     REMKNFPDNGQAEGHISSSPVTVGINTWSDTLTKPGYIYSYIVQSRDKEEMMSPGAAR
                     IIISLAKDSPIPPDYIKAKEIWIGKPAIMIEWAEVMYEGFTQIKLFRQSENKEAELIA
                     TLKPGNTHYVDKIEGKPETYSYFIKTVHSSGTESKPSATAKSGK"
     gene            complement(14227..15828)
                     /locus_tag="PKI35_01860"
     CDS             complement(14227..15828)
                     /locus_tag="PKI35_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HNX43152.1"
                     /translation="MSRRLKIPHTYVIVFIIIVIAAVFTWFVPGGAFDRETITTASGT
                     KEVIRTNSFHYVESAPQTWQIFSAFYKGFVRSPKIIVFILILGGAFWILNESKAIDMG
                     VYAFLKKSDKLNRYRFIRFLGVNNIIITVVMLMFSFFGAVFGMSEETIAFVIIFVPMA
                     ISMGYDSIVGVCMCFIGAGIGFAGCLMNPFTLGIAQEISGLQLFSGLEYRFVIWMVIN
                     IVGISYVLWYAGRVKKNPARSVVYKEDSYWREHHTGADMQVSVKSLPSTWVAFAGVLA
                     AIMVSAFIWPMNEIRLGANSFLFPALPLLAALFAVTGFLAARRSVQAFVLDILIFTIL
                     FLVVGVTGFEWGLLELAALFFAMALVAGIAMGKDANEITRSFVAGARDIMSAALVVGL
                     AGGIIVVLEDGRIIDTILYGISRAIEGTGKITTLSLMYVFQSGLNMIITSGTAKAALI
                     MPILRDISEMVNLSKQATVTAFHIGDGFTNLITPTSGVLIGVLEVARIPFNKWFSWAW
                     KLILILVILGWILLIPTVTMNLNGF"
     gene            16185..17252
                     /locus_tag="PKI35_01865"
     CDS             16185..17252
                     /locus_tag="PKI35_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43153.1"
                     /translation="MDSAQWLPANFHMHSRAWGGLTNGADTRDHEVYDTYKKIGFGSI
                     AISNYQKINALYSDKAFYIPAYEHGYGIYKNHHLCLGAKKVVWYDLPYGQNIHHKQYI
                     LNRLRPTTDILSINHPSFFKGYMPEDFTRLTGYDGIEVLNGYRNSIPHWDSALSAGRP
                     AFIFADDDMHDISVPDEIGRRFLVINSPSNSRSDILSALKSGKAYGVYYQGDKGESLT
                     EKSEKINHLPVITTFDVKNDTLSLTIDSTAYEVRFYGQHGRLLKKKGGIRKAFYALQE
                     DDSYVRAVILFPTRSSHEGLQYFLNPVLRSKDGKKPPMPQAEINQTGTWLSRIGLSLV
                     ALTFIWLSARIIKWRKEKRKS"
     gene            17292..18851
                     /locus_tag="PKI35_01870"
     CDS             17292..18851
                     /locus_tag="PKI35_01870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNX43154.1"
                     /translation="MPDSKKIKRYILLLLAFSAAIRAVLAASFALGNDEVYYWTYALY
                     PSLSYFDHPPMVAWLIRFFTANLTLNQEFFVRLASIVAGTLNTLLIFSLGRKLKDELT
                     GWYAALLYTASVYGFIITGTFILPDTPQTVFWLAALRLMTDVLPDHLLTTGSRRKMLL
                     TGILLGAGMLSKYSTAFLWLGMMIFILVKNRRWLAAWQFYAANILILLLFAPVILWNA
                     DNSFISFSFHSQRIALSEHLINFNSFVTELGGEMLYSNPVNVILFILALIAILRMPAR
                     RRNDGVRLLLWSGLPLILVFWIVSLFRSTLPHWSGPGYVSLIPVTAFWLRSLHENLFP
                     KAIKTSLAAAGVVLTVSLIQITTGIIPWPAGSDSYPEKGADDISLELTGWRTLGKEFT
                     ELSGKYEKSGLMPAGAPIISYRWFPAANLEYYAARPSKRFLLAAGPLNSIHQYAWTNT
                     LHGGFKLNTDAWYITSSRDFRPPAALKPLYFSHTSDPDTITVTRCGKAAYYFFVYRMK
                     DMQAKPAAIVE"
     gene            complement(18919..21282)
                     /locus_tag="PKI35_01875"
     CDS             complement(18919..21282)
                     /locus_tag="PKI35_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNX43155.1"
                     /translation="MKHILLVLAALWVPCFLFATDGNYSRVKIFFGDHDQRALYQAGI
                     EVQVIGKNGDWAIAELSDEELARVSQLGFSCEVLIPDLEAWYADRYVHPENYETTETD
                     RAVSEEWPVPANFQLGSCGGFSTIDQMLEQLDEMHTLYPGLITARMAVSDTSATCNGQ
                     VMYYVKISDNPGIDEEEPEVLYTGMHHAREPIAMQHLLFYMWYLLENYDSDPMIKALV
                     DTTEMYFIPVVNVDGYAYNISSNPGGGGMWRKNRRLNQDGSFGIDINRNYGYMWGNDN
                     QGSSPDPGNECFRGTAAFSEPETRMIKYFCEAHDFGIAINYHSYANKFLYAWGYTEDV
                     TPDESLFNNLSRIMTEENNFIYGPSSTTIYITNGGSDDWMYGEQSTKNKMLAWTPEVG
                     GDNDGFWPMQSRIIPLCQINMLQSLRAAQLVGNYAIVKDNSPVFVSKPSGYFSFNIKR
                     YGLTNGNFTVTVAPLGSSIASVGDAKIFTGMQLLQSIDDSIAYTLDPLIMSGDTIRYA
                     LILDNGSFSIADTIEKFYGMPVAIVNDSLNTTGNWTGQWALTSLKYVSPSKSMTDSPS
                     GNYSNNANRSTTTIVPVSLQNAMFAMLEFEAQWNLEAGYDYVQVKASTDDGLTWIPLT
                     GQYTRPGTINQAAGEPLYDGIQSEWVKETIYLNDFLDQNVLFRFTLRSDGGVQADGYY
                     FDDFKVSILLDPTKVEDKDEAVAGGLGTPFPNPADQEVNIPYVIPERCEAQILVYSQL
                     GSLMSSLPANSCTGICKVNVHGWPKGVYLVKLESADFPATVKKLIVD"
     gene            complement(21386..22000)
                     /gene="gldD"
                     /locus_tag="PKI35_01880"
     CDS             complement(21386..22000)
                     /gene="gldD"
                     /locus_tag="PKI35_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HNX43156.1"
                     /translation="MLRLKSKFMILFPCAVILTVLISCSGDYSPKPRGYFRIALPEKK
                     YTLLDSIYPYTFEYPVYARIHPDEMSPKEIYWINIDYPQFKGTIHISYRTIENNLQQL
                     TEDSRSLVLKHIPKSSGIFQKEIRDKENNVYGVCYEISGVGAASPYQFYLTDSTRNFL
                     RGALYFTTVPNNDSLSPVIDFVKQDIAHLISTFRWKNRASSGVK"
     gene            complement(22156..23517)
                     /gene="gldE"
                     /locus_tag="PKI35_01885"
     CDS             complement(22156..23517)
                     /gene="gldE"
                     /locus_tag="PKI35_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HNX43157.1"
                     /translation="MDYLFTGYSNLLFLILMIQPSGVQEVLNIIVLAALLIALLMCSA
                     LMSGSEIAFFSLSQSQLNELQSKKGTINQLIIKLLNRPKRLLATILIANNFVNVAIVM
                     ISTYFTGKFLDFSAFPVLGFIIQVVVITSLILLVGEMLPKVYATMNPVRLAKFMARPL
                     AFTVRLFHPLSVLLIGSTNIIDKRIERKKYPISMEELSDAIDITADGATDEDDRKILK
                     SITRFGDITVREIMRPRIDVTALEISEPFSQVKKVIVESGYSRIPTYDETPDKVTGVL
                     YIKDLLPHIARDDDFGWTKLVRNAFFVPENKPIDDLLEEFQAKKIHLAVVVDEYGGTS
                     GIVTMEDIIEEIVGEISDEFDSESDEPGYTRIDDHNFIFDGKTQLNDFCKILEISDRV
                     FEEAKGESDTLAGLLLELFGKIPRTNERITYGNFTFRIEAADKRRIKRVRVTLSEPDA
                     GEE"
     gene            complement(23615..24670)
                     /gene="mutY"
                     /locus_tag="PKI35_01890"
     CDS             complement(23615..24670)
                     /gene="mutY"
                     /locus_tag="PKI35_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNX43158.1"
                     /translation="MADIFLEWYADNRRDLPWRSSRDPYLVWLSEIILQQTRVSQGLS
                     YYNRFAGEYPTVFHLARASEPEVLKCWQGLGYYSRARNLYKTSKLVVEKYLGQFPSTS
                     AELVKLPGIGKYTAAAIASIAFGEAVPVIDGNVIRVISRLFGVTGDARSPSVYHLIND
                     TMKELMGNHPPGLFNQAVMEFGALQCTPLSPGCRECPLQLHCYAFDRQMVEQLPVKPS
                     KPVVSIRYFNYLVITFTGNGVETILLNKRTGRDIWHNLYDFPSVENDRLINPEELAAS
                     AEARELLGNDWTLLHVAGPVAHLLTHRKIIARFFRIHADYETPSMKRYRYVRIQDIRE
                     LPLPRLIEKYVGNHFPD"
     gene            24895..25179
                     /locus_tag="PKI35_01895"
     CDS             24895..25179
                     /locus_tag="PKI35_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNX43159.1"
                     /translation="MTKAEIVAEIANKTNIEKLAVQQTVEAFMDAIKDAMAHGENVYL
                     RGFGSFIVKKRAEKTGRNISKNTTIIIPAHNIPSFKPAKVFVNEVKVKVK"
     gene            25448..27004
                     /locus_tag="PKI35_01900"
     CDS             25448..27004
                     /locus_tag="PKI35_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNX43160.1"
                     /translation="MNKELIINAGPNEVNIALLEDKVLVELNKEKTNNNFAVGDVYLG
                     RVKKIMPGLNAAFVDVGYEKDAFLHYLDLGPQINSLIKFTRMGMSGNTAHLSMSEFET
                     APDIEKTGKIGQVLSSNQQILVQIAKEPISTKGPRISSEISFAGRYLVLVPFSNRISI
                     SQKIKSAEERNRLKRLIMSIRPKNYGVIIRTVAENKKVIDLDNDLNSLVQRWESIAAK
                     LPSAKPPQKILSELDRTSAILRDMLSDSFNNIHVDDSVLYEEVRNYIRTIAPDQVDIV
                     KLYKGKIPVFETFGVEKQIKNSFGKVITIKSGVYLIIEHTEALHVIDVNSGHKVNSEI
                     SQEENALAVNIEAAIEIARQLRLRDMGGIIVIDFIDMHDGRNRRTLYQKLKDEMAKDH
                     AKHSILPPSKFGLVQITRQRVRPETTVQVLEKCPACEGTGEIKPSIIIPDYIENNIRY
                     LLQDQNEQYLKVTVHPYLYAYLRQGGLFRSIQWKWFLTYKKWIRLMPVTAQHLLEYHF
                     FNRNQDEIKL"
     gene            complement(27126..29810)
                     /locus_tag="PKI35_01905"
     CDS             complement(27126..29810)
                     /locus_tag="PKI35_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNX43161.1"
                     /translation="MENCRILLVEDRQADIGLIKHQIENVLESYQVNSVNSKNDFVRQ
                     LNELNPHLVICSLDAASVDPVSIVRITRIKSRITPVIILYKAMKENLLTECMEAGATN
                     HCSKKQLNRLSQVIASALGNEIWGETAAYAKRNTASANVAGIPTGRETFFTTLFNNIP
                     DIYFQTDMMGDLYDISPSIASYLKYEKSELIGNSVFTLYWDARDRESLIQSLVKHGEM
                     RDFKIRFRTKTGEMKHGSIYARLLYNASGQPDLIVGTIRDTTGAVEAANSLRWNEEMY
                     RSIFDNQDTSILLIDLQEQKIVTANLAATKLYGWSTEEFLKINPRELFNITPHELANL
                     TESVKTTGNLHYETWHKCKNGTTKNVEVTTGKVIIMGKNYLWTAVRDVNSMKTIDQQA
                     QWMTIMAENSPVGLLVANPLGDIEYINQKYSAVTGLSLADVIGKNLWFRYYANNSGNT
                     FTEIWNMVQSGQSWSGECSTTGENGQPAWENVHIVPINNKHGQLIHIVVSNENIDEWH
                     KTMDELTSAKVRAEITDKMKTGFINNISDEIRSPLTGIIGFTEMLQNPDFSDETKMNF
                     MDIVSKSSLNLLNTINSYLDISMILSGKGTIHKKHCTVGTLLEKVKKEMKGVCEAGGI
                     TLNIIQPAAPDAIVLNTNPEILHKIFTHLVNNAVKFTQEGSIDVGFRLSKNTPGFFVA
                     DSGTGMDSDQVNSINDYFNLADTTAPYDFTGGNIGLSIVHGLVTILEGKIHLESTPGK
                     GSRFYFTLPAGAMATAVVSEKAPPAKIPVKENPVILVAEDNDFNYMYIEAVLNNAGYR
                     VIRAVNGLEAVEICHSNPEVSLVLMDLNMPLMDGREAIRQIKGFLPDLPVIVLTAYVS
                     FTSEEDAFLSGCQEFLKKPINRDDLLISVENTVHNRMN"
     gene            complement(30802..31443)
                     /locus_tag="PKI35_01910"
     CDS             complement(30802..31443)
                     /locus_tag="PKI35_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43162.1"
                     /translation="MLGDVLLITEKHRKAGEIIIEKILSGRKPKMIIGISGESGSGKS
                     ELAHVISKGLRSYGIFAKPLHIDNYYRILPLLRTEWRKQHGIENVVGYGEYDWDTIYR
                     NIQEFREGKISTGPCVDLVTEQVDQLTTDYSAIDMLVIDGLYAIKTEDVDLRIFIELT
                     YHETKKAQVVRGKEPQNEYRMAVLEKEHQMVQALKPMADVLIGMDYEVKAVKG"
     gene            complement(31522..34689)
                     /locus_tag="PKI35_01915"
     CDS             complement(31522..34689)
                     /locus_tag="PKI35_01915"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNX43163.1"
                     /translation="MPTPKFESVIFDLDGVITKTALVHGHAWKQMFDEYLKSRQQRFG
                     EPFSEFTHANDYLPYIDGKPRYDGVKSFLQSRKIEIPFGDPSDEPGKETVCGLGNRKN
                     VAFNEVLEREGVEVYDSTVKLMHTLLENNIHVGVASSSKNCYTVLKAARLLELIETRV
                     DGVVSSELGLKGKPEPDIFVTACNNLFCSPSRSVVIEDAVSGVAAGRKGNFGLVIGIA
                     RENNPAELYANGADIVVSDLEEIEIEDIDDWFGEGLASDNWSITYHTCEPEKEKTRET
                     LLTVGNGYLGTRGAMEESSAGNVHYPGTYIAGLYNRLITPVAGKNIENEDFVNIPNWL
                     PVNFRIGNGKWTNADDDEITYIKRRLDFRNGLFTRHLEIRDEFGNETLIISDRFASMS
                     DRHLAALRYTIIPLNYSDRLTVRTGINGDIINDGVERYKSLNQKHLKPVEQRAEGNIQ
                     QLLVETVQSGVKIAVSSVCRFYAGPAPQNHHLNPEIQPGMVSGETSFDVEAGNLYTLV
                     KKVAVYTSKKDDVTDPMAESHKAVTNAPDFESLLEESTNAWARIWDKIDLKITGDRFS
                     QKLIRLHLYHLMVSMSPNNRKIDAGITARGLHGEAYRGHVFWDELFILPLYDLQQPEV
                     ARAMLMYRYNRLGKARAYAEEHGYRGAMFPWQSGSDGREETQVIHLNPLNGEWDPDHS
                     SLQRHISLAIAYNIWQYYSITLDADFMRRYGAEMYFEICRFWERKSEWNEKSGRYDIR
                     KVMGPDEFHEQYPGSAEGGLTNNAYTNLMAAWMFSKTGEILAAIGEEGTASVKTAIGL
                     KDQELKHWDDISQKLVIPINEDGIIAQYEGYFDLKELDWDFYKSKYGNIYRMDRILKS
                     EGLSPDAFKVSKQADALMTFYNLDKETVDRLFSRLGYSLPADYLQRNLEYYFRRTSHG
                     STLSKVVHSKLAALAGNHALSRELYNDALASDYTDIQGGTTAEGIHAGVMAGTVLIAI
                     TAYAGINFGGEILKISPALPETWEMMEFGFVFRGVAYRFRVCHDSITIDTDKEAEIEL
                     KGKKYELWKDIPTTFGLKSKDAFSK"
     gene            complement(34819..36507)
                     /locus_tag="PKI35_01920"
     CDS             complement(34819..36507)
                     /locus_tag="PKI35_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNX43164.1"
                     /translation="MEAVCDVLTQYWNRLYPGVSTAILNNFLHSADAYRECGKNEKPE
                     TDWYKDAIVYSVYVDLFNNDFQGLIDKLDYIQELGVNCLWLLPVLDSPMRDAGFDISD
                     YYNIRQDLAGNSPLGRTGVFDLFLTEAHHRGIRVIFDIALNHISDQHPWFTEASKGPD
                     NPYRQFFIWNSSPDKYKDARIIFKGIENSNWEKCGEEYFFHRFFNFQPDLNYKNPEVL
                     LAMCRHLLYWQNRGVDGFRADAIPYIWKEEGTACENHPLTHVVVKFFRAVLDYIKPGT
                     LLLAEACQQPYKVVQYLGDGDECHAAYHFPLMPRIFKAIAMQNGKPVEEILGPAVTPE
                     LPPSGQWFTFLRCHDELSLELVYVTEEERKFIHENYCYNPKWDFRLGEGIAARLSELM
                     QRNPRKIHLAYSIMLTLPGTPVIYYGDEYGKLNDEEYYAEMIKLAGKDDTRFLARGKI
                     DWGKLETQLNDSNSFISRVYYPTKQMLGVRRHFKAFGRGTLRFIEMKDTISGNMLAFY
                     RQYGDEKLFIIHNMTNLALPLSLPEDAASGKDLFGKPLSGEIEPLGFRWITIES"
     gene            complement(36625..37614)
                     /locus_tag="PKI35_01925"
     CDS             complement(36625..37614)
                     /locus_tag="PKI35_01925"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HNX43165.1"
                     /translation="MKNKIWILLTIIGLTTLISCEKDETKILLSASPTAPTLSLPSDN
                     IVLKRTEASDTLSFTGALAEYGFVAAESYKLEVISATGSFDSPTVVATTTNANSFKVT
                     VGDFNAKMLSVPLNADESATIKVRVTSYGLPGVDDLHSPELTMTVTPYGYPKLVLSTT
                     PSQNIYSQLGDGNYTGFAKLPAGTPFTMTDPDAGKVYGGSGGVLVENGDSFSVSTEGW
                     YKISASTVNMDYTIKSFMIGLVGSATPNGWNAPDLKMDYNATGNYWYITTDLVAGEVK
                     FRMNDAWDWNLGGTESSLSQGGANLPISEAGNYTIKLYLSANEQSGSCTIVKN"
     gene            complement(37634..39838)
                     /locus_tag="PKI35_01930"
     CDS             complement(37634..39838)
                     /locus_tag="PKI35_01930"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX43166.1"
                     /translation="MKRILHITLFMAGIALLMVSCIKDLDTLPIDPDVISAEDVYKDP
                     ASYKMVLAKLYSGLAVTGQVGAAGNPDIEGIDEGFGEYLRAFWYHQELTTDEAVIGWN
                     DQTIKDFHFQKWGSSDVFVAAMYSRIFAQIAFCNEFLRQTTDAKLDERGVTGSLRSDV
                     ALYRAEARFLRALSYYHAVDLFANTPFVTENDPVGKFFPEQKTRADLYGFVESELLDI
                     QSQLKDPGANEYGRADKAAAWTLLAKLYLNAEVYIGTPKYTECITYASKVINAGYSLE
                     PEYENLFKADNQKSNEIIFPIVFDGLRTRTWGGTTFIVHAAVGGSMVPADFGIGGGWG
                     GIRTTSALVDKFEGSVFPKSLRKGIKNAYPVLNCPGSYQGWNPADPNTVLASVNSDNK
                     YEGYLYFKDAGTQFKFALGSWDTNWGDTGADGTLEPGGDNIQVADAGYYKVNVDLNAL
                     TYTIIKTDWGIIGSATSGGWGSDQNMTYVPDENNNQAGIWTATVDLVQGEIKFRANDG
                     WDINYGDNGNNGTLEAGGDNIPVAEPGSYVITMKLGAPDYTYTVTRQSIDKRAMFWTD
                     GQSLVIKDISDFTNGYAITKWKNVTSTGAAGSDNTYVDTDFPLFRLADVYLMYAEAVL
                     RGGSGGDAGTALDLVNAIRTRAYGDTHGNITAGELTLDFILDERARELYWECTRRTDL
                     VRFGRFSSGDYVWPWKGGIAEGIETDKHFNIFPIPSSDMTANPNIKNQNPGY"
     gene            complement(39859..42825)
                     /locus_tag="PKI35_01935"
     CDS             complement(39859..42825)
                     /locus_tag="PKI35_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX43167.1"
                     /translation="MKKQSQLLRVLLLLLSWAVTTIAMAQEVKISGKVTDASDNSTLP
                     GVSILVKGTTTGTVTDLDGRFALTVRPDATLVLSFIGFTTQEVAVGSQRIFNIALQPA
                     VTGLSEVVVIGYGQVRKGDATGALTALGDKDFNKGAISSPQQLLVGKVAGVTVTTAGG
                     APGGDATIRIRGGSSLRASNEPLVIIDGVPVEKGKNFDGTSTISGVRSPLSMVNPNEI
                     ETFTVLKDASATAIYGSRASNGVILITTKKGKAGDKLHVEYSGNLTYSTIPKVIDVLS
                     ADDFIKLENEKYPNFTSLLGDAKTDWQKEIYQNAFSTDHDVSVTGQFHEMPFRVSAGY
                     MWQDGILKTDNMKRTTMGISLNPSLLDDHLKVNMNGKWMIVHNRFADNAAINQAIQFD
                     PTHPVTAVNENGGYWAWLQTNGEPVDQATDNPVATLMLKNDQADALRFVGNMQFDYKF
                     HFLPDLRANMNIGYDYSGSDGTVYVPKYATLNYNDGGIDRVYDQTKENSVFDFYLNYV
                     KDVKSIYSKFDVMGGYSWQHFYSWINTYHNNVDHTAPKTEIDANEYFLVSFFGRFNYT
                     LLDRYLLTFTCRDDGSSRFSKDNRWGLFPSAALAWKINEESFLKDVKQVSQLKLRVGW
                     GLTGQQDISDNAYPYIPVYTTSDVWTSYLLGNTFYYTLRPEEYDANIKWEETETRNIG
                     LDFGFFNDRITGSVEYYERYTTDLINRITFPAGSNLSNYVTTNIGDMENKGWEFSLVG
                     RPVTSEKLNWEIGANLTINKNEITKLTTVDDPNYTGVLVGNIQGGVGNTVQVHSVGYP
                     MFSYYVYKQVYDQDGKPIQGLYADLNNDGIINDNDRYRYKSPNADYVIGISSKLTYKK
                     FDLSFSGHANIGNYVYDNVNSENGVYERLYRSEGPYLSNITSSVFDVDFTSPQYLSDY
                     FIKNASFFKMDYITLTYMVGNLYKNTANMTLSFTVNNVFTITKYKGIDPEIFDGLDKQ
                     IYPRPRIFALGVNVLF"
     gene            43477..44730
                     /locus_tag="PKI35_01940"
     CDS             43477..44730
                     /locus_tag="PKI35_01940"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HNX43168.1"
                     /translation="MATIPKLFVPKGNRNSPLRLILILPLLFLMAGCEKKDTSAPRNN
                     RQPVYYTIGQFCMGCDLSVVNQVEDHGGVFADSSVAGDPYSILHSHGANLVRLRLWHN
                     PQWTKEVYGAEGHQLYNDLADVEKSMFRAKQNGMKVCLDFHYSDTWADAGAQEVPAAW
                     AGITSLDVLKDSVYNYTFRVLSYLKNESLMPYMVQIGNEINCGMCYSGADADFPNLNA
                     CQGHWAELGGVINSAINAVRDVSVGNTNEVLVALHIADPSNVEWWLDNITTTGGVSDY
                     DIIGFSYYPLWHTGIKLSNISYVTGRVREKYGKQVMVFETAYPWTADGNDNYTNLLGG
                     QTPVSGYPFTQQGQLDFMKTLTQEVIDGGGIGVIYWEPAWITSQMKDLWGSGSSWENA
                     TFFDFSGNTLPVIDYMNYPYTFPGK"
     gene            44783..45796
                     /locus_tag="PKI35_01945"
     CDS             44783..45796
                     /locus_tag="PKI35_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNX43169.1"
                     /translation="MPKSAPLTIKDIARNLGVSVSTVSRALKDHPDISPETKLMIQAY
                     AEQGNYRPNVLALSLRRQKSYTIGLIIPAIVHHFFSSVISGMEELAYKEGYNLIICQS
                     NENQHREAINLQTLIDHRVDGILVSLSKTTQSFDPFIEAFASGLPIVFFDRVCPEVET
                     DRILTDDFHGGRIATEHLIKRGCKNILHLAAPQHLLIGKGRFDGYCEALKSKGLAFRN
                     EFTLKCDTPEEVMALKNHLIDLAPHIDGIFAVNDSTAIAVIQVLQKAGFSVPDDIAVV
                     GFGDGPNATIIHPELTTVVQKGYEMGRAAVRLLLQRLNNPSAAISPQTISFKPVMKIR
                     ESA"
     gene            complement(45860..46792)
                     /locus_tag="PKI35_01950"
     CDS             complement(45860..46792)
                     /locus_tag="PKI35_01950"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNX43170.1"
                     /translation="MRIYHSISEFASATKPVATVGVFDGVHRGHMEIINHLVASAKRL
                     QTESVIVTFEPHPRLVLDTYQQVKLLQTLDEKLERFELAGVDAAVIIPFDKSFAGTDP
                     QQFIRFVLIDSLHVQKIITGHDHMFGRNREGDYALLVEIGAASGVEIEQVKAVSYCGE
                     EVSSTSIRKALAAGNVKLAGCMLGYPYSFSGKVYRGNQIGKLIHFPTANIEPDSPYKI
                     IPANGVYASLVEWNGKRFKGMSNIGTRPTINANHLTIEVNIFDFDTEIYFEKISLSFI
                     ERIRDERKFGGLEQLQHQLSLDKEVAMQLLEETF"
     gene            complement(46856..47857)
                     /locus_tag="PKI35_01955"
     CDS             complement(46856..47857)
                     /locus_tag="PKI35_01955"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNX43171.1"
                     /translation="MKIAIVGATGLVGSVMMKVLEEARYLSFDKISEVIPVASERSLG
                     KVLHFNSMPYPVVSITEAIEQHPEIAIFSAGAAVSREFAPQFAAAGTTVIDNSSAWRM
                     NADIPLIVPEINAEILTPSHKIIANPNCSTIQMALTLAPLHKAFTIKRVVVSTYQSVT
                     GTGQKAVVQLNNERAGISGERVYPHPIDMNLFPHGGTFESNGYTSEEIKLLNETRKIL
                     GDNSINVTATVVRVPVTGGHSEAVNIEFVNDFDLKAVFSLLSKTKGIVVQDDPEKSIY
                     PMPLYAEGRDEVFVGRIRRDESQPKSLNLWIVSDNLRKGAATNAVQIAAYLAEHELV"
     gene            47932..50529
                     /locus_tag="PKI35_01960"
     CDS             47932..50529
                     /locus_tag="PKI35_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNX43172.1"
                     /translation="MKKKHFTIILLLLSCRVQAQVIDDFSDGDFTSSPSWTGDIASFK
                     VNTSFELQLNSSGDNISALATACPMADSMEWNFKVRLAFSPSDNNNARIYLVSDQQDL
                     KSNLNGYYLKLGESGSDDAIELIKQSGNIHTSLCRGTPGLLATAFTLRIRVIRDNSGN
                     WSIFADPSGGNAYHPEATATDDTYTSASWLGIYCKYTSSNSTKFYFDDFYAGTVIIDK
                     TPPGLLSASLLTSNALDLRFSESIDLITGQDVANYTLDHGIGHPAIVLRDAQDLTLVH
                     LLFSPELTSDITYHLTIEGIKDLSGNEIVALDVPVSWHQVKLFDVLINEIMADPDPPT
                     GLPESEYIELYNRSTFPVNLEGWMIRVGSSEKDFPAYTLPAGGFVILAANSSEPSLTG
                     YGPFIGFSGFTIVNTGTEITLFDKDRHVIHSISFTEDWYRNDYKKEGGWSLEQIDPLN
                     PCGEAANWIASNDASGGTPGKMNNANASNSDNTRPVIQRIGVDDPWHIPVWFSEPMDS
                     TTLLDPSNCIIDQGIGNPASVTLSGPDYRKITMLLANPIVQGTIYTLTIEGSFTDCAG
                     NMISGTAMAKFAIPSATKANELIINEVLFNPAPSCTDFVEIYNRSANVFDLQWLTLAG
                     YDTVTLGLSDISRICQESRLIFPGEYYAVTTDTAAVKACYLTTNPGGFIQVSGLPSMN
                     DDEGAIALALGTGEIIDRMIYSDKMHYPLLTGTEGVSLERINPEQPSSILSNWHSASE
                     TVGYATPAYQNSQYGQVAQSGNEISLSPSVFSPDNDGKDDYLTISYTFEQSGNNARVS
                     VYDATGRLVRRLVNNELCGTNGAFTWDGFTDDRTLALAGRYIIYIDIFDSEGKVKHFK
                     KTTVLGCKL"
     gene            complement(50533..51234)
                     /locus_tag="PKI35_01965"
     CDS             complement(50533..51234)
                     /locus_tag="PKI35_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNX43173.1"
                     /translation="MMKVLSVFRDFISFFYPDTCYACGNSLFRHEKVLCTNCLQHLPK
                     TNYHLHPGNPLDRVFWGRVRVEQATALYYFSKGGNVQHLIHQLKYKGKKEVGIYLGKL
                     LGNELKELPAYHDISAVLPVPLHPRKQRKRGYNQSEQFAIGLSEAMGIRMEAGSFIRK
                     VATETQTKKSRLARWENVKEIFAVTDSSRLEGRHLLLVDDVVTTGATIESCANVLLAL
                     PGVKVSVASIAFAHE"
     gene            complement(51253..52392)
                     /gene="amrS"
                     /locus_tag="PKI35_01970"
     CDS             complement(51253..52392)
                     /gene="amrS"
                     /locus_tag="PKI35_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNX43174.1"
                     /translation="MDKRAFLKSGLAVLGCAACGLNHRQVSALSLDRELPAAANGLWK
                     WSREAYHYRITDRGVQCTNCPNECTLKRKETSKCRNRINIGDKLYSIAYGNPCAVHID
                     PIEKKPFFHFFPTSQAFSIATAGCNFSCLNCQNWEISQKSPKETSNYDLMPAKVVDEC
                     LKKKCRIIAYTYAEPTVFYEYVYDTAVIARRQGLKNVYKSNGYINEKPLRMLCKYLDA
                     ANIDLKGFSENTYQKLNSGKLAPVLRTLKILQEEGVWLEITNLIVPTWTDNPDIIRSM
                     CNWLVKNGLDDCPLHFSRFTPLYKLTQLPPTPVSILQKAREIALSEGMNYVYVGNLPG
                     TDMENTRCPKCKKLLVIRKGFMVVTNYIVNGACKFCGQKIPGRWS"
     gene            complement(52536..55106)
                     /locus_tag="PKI35_01975"
     CDS             complement(52536..55106)
                     /locus_tag="PKI35_01975"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNX43175.1"
                     /translation="MIDKPYAILSIPLLCLVLYFASLLMVYLGIISRITHRKYWNVLL
                     LAAFLGAAGLGFLLALQVNLKFDLPAAATFLICHVDFGIGLLVIASLHLSWHLKYFLE
                     IIKPRSRVQKSKQKGIAQITDDQKDPFDKRQSGEKTIFLLLPFSLGLTAMASQIILLR
                     EFLAVFNGNELEIGVILANWMLLTGLGAWLGKFVHHEKRQTSRIAFTLLALSILAPVT
                     LFILDSMRNVVFTTGSMAGIYNVFLMSVFLLAPFCLLSGSLFTRLSSIMTFHSKDPAT
                     GLVYSWENIGSVTGGALFNFILVFFLKPFQALTVIVIVNALILGWFLFKERQKSLLPG
                     IAVISIVFVALVLSVNPDTVTRKFLFPGQKILTYRDTPFGNMVVTESGGQKNVYENNT
                     LLYTTNNVITNEETVHYAMLQRKNPVNVLLIGGGFSGIVGEILKYPIKDLDNIEMNPW
                     LAQVAGTLSEMPEDDRLKVVLSDPRSFTDRLVKREATHNNGAGSSRYDVILLNLPDPS
                     TLQLNRFYSVEFMTILKPLLRKGGVVSLSLMATADYMGYSSKRAHLIMYATLKKVFKN
                     VLVVPGDRDYFLGSDSALRIDMASLQTERGVETDYVNPYYLEDESLQQRSRTIMQDLQ
                     PGAPINRDFKPLAYQSQVKYCLDYFGHDLGYLPLLLLLLLLPVLFRSDSTTVGLFTAG
                     FAASASEVIILLAFQVIYGYIFLAAGMIITVFMLGLALGAAYARKYFRIVSSIFLVRL
                     QFAMAICILVILVAIIGFHRFHSTTVAIHAGFAILTLMLSILTGMIFQVAAVVKQGEV
                     RSVAGSLYSADLLGSAIGALIVSTWLMPMLGLSGTLMAISGACLLSVLIMALKRKRAG
                     "
BASE COUNT        14779 a        13581 c        12956 g        14700 t
ORIGIN      
        1 ctcacgaacc tgagcaccgg tccgtctaac tggcgatatg taaaagccga ctggggcgtc
       61 aacattcccg actgcaaact tattccgctg ggaaacgaca aatggcaact gaacattggc
      121 ccgaccatcc gggattatta cggcgtgcct tccgcagaac agattctgca actggctttc
      181 gtattccgaa gcggcacaaa agtgaacggc tcttaccttg aaggaaaaac ggcttcggga
      241 ggcgacattt tctatgatgt ttacccggct gcattaagca tcaagatcac ctctcccgat
      301 gaagatctcg tctttgtcca gcttaatgaa acgtttaccg tgaaggtggc ctcttctttt
      361 gccgacagca cttttctgtt cgtcgacggg acaagggtag cagccaccac ggccgactcg
      421 ctggtttata acattactcc tgatgcgtac agccgggttc tggtaaaagc ccgggccgtg
      481 aacgatacag ccgtggttgc cgattcgttc tactattacg tccgcggccc ggtaccggtt
      541 gcggaattgc ccgaaggtgt gaccgaaggc atcaattaca tcgacagcac ttcagcggtg
      601 ctttgcctgt acgctcctat gaaggaatac gcttttgtta tcggcgattt caacaactgg
      661 ctccccgacg aactctccta tatgaaacaa acgcccgacg gcaaccgctt ctggatacag
      721 attgacgggc tggttcctgg taaagaatat atattccagt accttgtcga cgggaacata
      781 cgcatcggcg acccttatgc ggaaaaagtg agcgatccct ggaacgattc ctacatcgat
      841 gactcaacct acccggatat gcttgcctat cccacgggaa aaaccacggg aatagccact
      901 gtgctgcaaa cccaaaaacc cgggtatgaa tggaaaaaca tatcctttac tccccccctc
      961 gctaccgacc tggtgatcta tgaattgctg atccgggatt ttacttccca acataccttt
     1021 cagtcggtca tcgacaccct cacctatctg aaacagttgg ggatcaacgc catagaattt
     1081 atgcctgtca gcgaatttga aggcaacagc agttggggct acaatccgaa ttattattgc
     1141 gcggtggata aatattacgg tccggaaaac aaattcaggg aacttatcga ttcctgccac
     1201 agcaatggca tcgccgtgat catggatgtg gtcttcaacc atgctttcgg cacatcgcct
     1261 tatgtgatgc tttactggga caaggctaac aaccgtccgg ccacaaacag ccccttctat
     1321 aatccggtgg ccaagcatcc ttacaatgta ggttatgata tgaatcacga gtcggctgat
     1381 tcgaaatact acatcagccg ggtgatgagg cactggctca ccagatacaa cgtcgacggc
     1441 ttccgtttcg atctttcaaa gggttttacg cagaattata ctaccgatgt cggcatctgg
     1501 ggccattacg atgcctcgcg catcagcatc cttactgctt accacgacac ccttcaggct
     1561 gttaaacctg aggcatacat gatcctggaa cactttgccg acaacgatga agaatcggtg
     1621 ctggccgcca gggggatgat gctgtgggga aattattcgg gacaatacgg cagtgcagca
     1681 gccggcagtg ttgcagcagg aaattttcca ggcatttcct atgtttctca cggatggttc
     1741 aagcctaacc tggtcgggta tatggaaagc catgacgaag aaaggctgat gtaccggtgc
     1801 atttcggaag gctacgtttc ttctgcctat aatataaggg atacggttac agcgttgaaa
     1861 cgcgccggac tggccgccac tttctttttc acggttcccg gtcctaaaat ggtgtggcag
     1921 ttcggtgaac agggttatga ctattccatc aactggcctt cgggatctgt caacgaccgg
     1981 ctaaccccaa aacctccaag gtgggattac ccggatcagc acaaccggct cctgctcctt
     2041 tatgattaca tcagccttat taaactgaaa accaccaacc cgttgttcag gacaactgac
     2101 ttttcgctgg acgtaagcgg cgccatgaaa aagataaaac ttcgtaatga agaaatgcag
     2161 gcagtagtgc tgggcaattt cgatgttaaa cagggaaaca tctcccctgg tttttaccat
     2221 accggaacct ggtacgatta tatgaccggc gactccatcg ttgtgtcaaa tgcagattca
     2281 gctataaccc ttgccgccgg tgagtacaaa ctttatcttt caaaacgcat aaacaatccc
     2341 tggggcattt ccgggaaaca ggtagcggat gtaaccgtcg atatagctcc aaatcccgtg
     2401 attgagcaga cgacaatcag ggttgcagcc ccgggtatga aaagatgtat cattcgttcc
     2461 ttcgatatga ccggcaggtt taccggcacc ctttttaacg gaaccttctg caaccagatg
     2521 gaaatcacct ggcagccttt gcataaagga ctgaacatcc tggttattga agccgggagc
     2581 gctgtcacaa taaaaaaagt cctcgtttta taggcggaaa cgaagccagg ttcccccggt
     2641 aaggataata ccctgcctgg caggcgatca ggaaaaagat ggcaggctta acgggattca
     2701 gattttttct ccatgataac ggctacttca tgaattccgg gcgtctggaa cttggggttc
     2761 aggttttcga cagagatggt gcaggctccg gtatccgaaa tttcgatgcc ttcccataat
     2821 ttctgcgtaa tcgtatagta tccgtctttg ctggcgccac aaaacttttc cttatccctg
     2881 attttaagcg ggacttcgtt catccgttct tcacccgaag gcgtatccag gataacgtaa
     2941 acgggaaact catcgcactc caggccgtcg gtatgtttca gcaccagcga aatatcgtaa
     3001 ctccccggct tgagttcttt cggcatctcg aacagcaact tgttgaacct gtcccaggta
     3061 tcattcgtaa aggtattttt cagttcgtac ttcccgcccg gtttgcatgc cagaagcagg
     3121 ctgaaaagta aaaaaaacgg cagtgtccgc atccttttca ttttctatag atctgttaat
     3181 tttttcacgt tagtcgatca ctccgctgcc aaggcaaatg cggtgttctt catcataaac
     3241 cactccaaac tggccgcagg cgataccgga aaccggaaca gaggatttga taaccaggtg
     3301 cctgccatcc tgttgaaggg tacccgcagt aaaatcaggc gtatggcgga tcttgaaagt
     3361 tatccgctgt ggctggatca gaccgggcca gggatttttg gtgataaaat ggaaatcggc
     3421 caggcggatt tcagaaccat actgagccag cggatcatag ccgttcgata catagataat
     3481 gttctgatcc gtatcctttt tcaccacgaa ccacggtccc tggctcaggc ccaggccttt
     3541 tcgctggcct atggtatgaa accagaatcc cttgtgtttg ccgagaattt tcccggtttc
     3601 cagttctatg atcggtcctt ctttttcccc gatgtatctc ctgaggaaat caacataatt
     3661 cactttcccc aggaaacata ttccctgact gtcgcggcgg tgggcgctgg gcatatgctg
     3721 actgcgggca atagcgcgaa cttcgctctt catcatattt ccgaccggga acatcagttt
     3781 gctcagttgc tcataggtaa tctgcgccag gaaatcggtc tggtctttca cgggatcgat
     3841 ggccgtcccc agccaggtaa ccccgtcgga ttcgatggta gttgcatagt ggccggtagc
     3901 aattttatcg aagtccctgc cccactgctt ttcaaactct ccgaatttga tcagccggtt
     3961 gcacatcaca tccgggtttg gcgtcagccc ttttttaacg gcatccaggg cataagccat
     4021 cacattatcc cagtactcct tgtgcaacgg aacgacttcc attttcagcc cgtaatggtt
     4081 agccagccag gaggtaagtt cgatgtcctc ttccgaggag caatcaagaa aatgctcgtc
     4141 ctccttcatc cctatccgga tgtagaagat ggtaggttca aagccctgtt ccttcaacag
     4201 atgaagcata acagaactgt cgactccgcc tgaaaccagt gcagcaatct tcatttatgg
     4261 gctgtttgag aatgcaaaat tgcaaaaaaa agggctacgg tagcccgcgc ccttattaga
     4321 gatgttatat tttattctag atcaatcctt tggctttctt ttcacgaata gccttcagca
     4381 tatcgatggt cattttttcg aggttccatt caggagtcca tccccactct tcgcgggcgc
     4441 aactgtcgtc cattttattg ggccatgaat tggcaatgga ttgtttaaca ggctcaacct
     4501 gatattccat ggcaaatgcg ggaatatgtt ttttaatttc agccgaaata atttccgggt
     4561 caaagctcat agcggtgacg ttgaatgaat tgcggtgctt cagtttcgaa ggatcggctt
     4621 ccatcaggtc tatagctgca ttcagggcgt cgggcatgta catcatgtcg aggaatgtac
     4681 cggctttcag cgggcaggta tatttgcctg ttttaacggc ttcataatag atctccacgg
     4741 cgtaatcggt agttccgcca ccggggagag taacgttgct gatgatgccg gggaaacgga
     4801 ccgaacgggt atcaacgccg aatttcctga aatagtagtc gctcagcagt tcgccggtta
     4861 ctttggtgat gccgtacatg gtttgcggac gttggatggt atcctggggc gtgttgtcaa
     4921 ggggtgtacc gccaccgaag gcgccgatcg agctgggggt aaaaacggca caaccgtttt
     4981 cgcgggccac ttcaaggcag ttcattaatc cgccaatccc cacgtgccag gccataagcg
     5041 gtttggattc acctactgcc gaaagtatag ctgccaggtt atagatggtg tcgatcttat
     5101 atttcttcac aacatcagcc acctgttgtg cattggtggc atcagccact tctgctggtc
     5161 ccgattcgag cagttcaccg gtgggttttg ttgcacggaa gccggcaacc acattgctgt
     5221 tgccataacg tttgcggagt tccagggtca gttccgatcc gatctgtccg actgccccga
     5281 ttacgagaat atttttcatg aataagattg atttatttgt tattcgatta acaatgcagt
     5341 ggcaaaaata atgaaaatta accaagtcaa agatgatatt cggcgcaaat ttcctccggc
     5401 ctttcgccct gattttcacc ggtcaaaggc aaagcggcga taacagataa attaacagat
     5461 tttaatactg aaataatatc aaaaacaact gtacgttttt acaagattta tatctttgca
     5521 cattgttaaa ttaagaacaa taattctacc taacatctta aaatacagat aattatgtac
     5581 gaaaattttc agtctttcct ccagaaagaa ctcaccggca tcgaagaagc cggcctgttt
     5641 aagaatgaac gcatcattgt gtcgccacag ggtgccgaaa tcaaaatcaa cacaggtgct
     5701 accgtgctga atttctgtgc caacaactac cttggactca gcaacaatcc caaactcatc
     5761 gccgctgcaa aaaaagctct tgacagccac ggatatggaa tgtcatcggt aaggtttatc
     5821 tgcggtactt ccgaccttca caaagaactg gagcgtaaga tctctgaatt tttcggaaca
     5881 gaagatacga ttctgtacgc tgcctgtttc gatgccaatg gcggagtatt tgaaccattg
     5941 ctcggagaac aggatgccat catcagcgat tcactcaacc atgcatccat catcgatggc
     6001 gttcgtctct gcaaggctgt tcgctatcgt tatgccaacg ccgatatggc agacctcgaa
     6061 aaacaacttc aggctgccca ggcccagcgt ttccgccttg ttgttaccga cggcgttttc
     6121 agtatggacg gcaatgttgc ccctctcgac aagatttacg aattagccaa taaatatgat
     6181 gccatggtaa tggttgacga aagccattcc gcaggagttg ttggtcgtac cggtcgtggt
     6241 gtaaccgaac aattcaactt gagaggcaag atcgaaatcc ttaccggaac tctcggcaaa
     6301 tccttcggcg gcgctatcgg cggatttaca accggtaaaa aagaagtgat tgctatgctt
     6361 cgccagcgtt cacgtcctta tctgttcagc aattcagttc ctccgctggt agcagcagcc
     6421 ggtatcagaa tggttgaaat gatgagcgaa accaacgaac ttcaggataa gcttcacgaa
     6481 aacacggctt actttgttaa aaagatgatg gctgccggtt tcgatatcaa accaacccag
     6541 agtgctattt gcgccgttat gctgtacgat gccaaactga gccaggaaat ggcagcaaaa
     6601 ctgcttgacg aaggtattta tgtaaccggt ttctattacc cggttgttcc caaaggccag
     6661 gcacgtatcc gcgtccagat ttcagccgct cacgaacacg cacaccttga caaatgcgtt
     6721 gccgccttca cgaaagtggg taaagaactc ggcgtattga aataatcctg ctgcaggaag
     6781 taccggttat cagaaggctg gcgccttttc ataccgatac tccttcctat acagataaaa
     6841 ggctgccccc gggcggcctt ttcttttact agatatttat caaccgaata gtggtatctt
     6901 ccgtttaaat tgtcatattt gtaaccagat tatttaatca ttttaaaatt ccgatattat
     6961 gaagcgtatt ttaccttttc tgcttttcct aggtgttttt tcgtttgcaa tttcttacgg
     7021 tagcctggca caaaaaagcg gcagtactcc gggcgccgca ggagtgcagg ccgccaatgc
     7081 caatttcagc aggctgcctt tagcaccttc catctattgg gcaccgacct ctttggtgga
     7141 tacagtctgg acaggacttg acaataacca gcctctttat ctgatcaaca atggagatac
     7201 tgcgctcaat ttcatgtttc caaactattt tgagcccaac ccggtcgtga gtccgtacac
     7261 atactgttca gcctcgggcg ggtgcgatga atttatcagt actgtcgcca ttaactcgat
     7321 tacaaacagc agcggctgca cgggctatgg caattacacc tctgtttaca ctcatcttaa
     7381 tacggggatg tcctatcccg ttacggttac tctcggccct ccttcttata gccaggatgt
     7441 caccggggta tggatcgatt ttgaccatag cagcaccttt gatgaggatg agttaaccca
     7501 gctttcagga tatttaccgg caaccggcgt cattactatt cctgctgatg cctctttagg
     7561 cgttacaacc atgaggataa gaactcagta cggaggcacc ttatctccct gtggaactac
     7621 tgattatggc gaagtggaag attacacggt atgtatcaga aagccttcct ttgtcactga
     7681 tatttatcct gtttcaggaa tgattgaacc gggtgataca gtctctgtaa atattaccac
     7741 gtcggcaacc ggcaattatg ctgcttatgg cgtttattac gataccctgc tgctttccac
     7801 caatgatact gtaaatccgg caatcaatat tcctgctgaa atgcatgtgc tgggatatgg
     7861 cgaactgcag ggctcattaa tagactatgt tacacatgtt cctttaccgg gtataatggt
     7921 gaaggcttta cagaacatca ccatgaccga cgattcgggg cattattccc tcagcctgat
     7981 ggaagggcct tacgaagtga aatttacacg gcctggttac gatacaatag tagctcctct
     8041 gacaattata cgcgacacta ctgtaattta caacgattcg ctgcatttct atccctaccg
     8101 tccggcgtgt gcatatgctg aagtgatcaa cggggattcg gcctgccaga ttacctggtg
     8161 caagcctgcc ggtcctcaac tgctgctcta tgacgacgcc aatgccgaaa attactttac
     8221 ctgggacagc atttgcagga tcaatgcggt gaggtttacc cccgatgact atccggcaac
     8281 cctgacagga ggacagttct atctcggttc cataccatat gacgggctgc cgtttaccgt
     8341 ggctgtttac gatgacgacg gagccggagg cttacccgga acccttctcg attcggttag
     8401 ttgtacaacg gtattcgccc ccgatggctg gatcacggta aacgggctga atgcaaccat
     8461 ctcttccggt gattattacc tgagcattct gcaccagtgc gatagtgcta gctgcataac
     8521 gttgggcgtt gacgaaacag atcctgatgc cggcaggagt tatacccgca atatgacggc
     8581 cggcgagcca tggatgcttt ccaccgttca ggattttatg atccatgcca ttgtggacga
     8641 acctgtgccg ccttacagcg gtaaggctct tgcccggaat agcggttccg gtatgacgga
     8701 cggattaaaa tcatccggca acgatgatgc atcgccagcc ggcaacaatg atgcgttgga
     8761 aggtttgtct cattatatgc tctgcagagc acctgttatc gatccgcttg ctggtccgca
     8821 atacggaaca gatattctat tagccaacga tctgattgat acatccttcc ttgaaggcgg
     8881 tacaacctgg atttgtctgc cagccggatg gtatgcatat ggcattcgtg gcatttattc
     8941 tgatggttca gcttcagaat ggcgtttcac ggaaccggtt gcccataaat tgtatgccga
     9001 cgtcagcgtc accgtgcttt cggaatgcaa tacgatgccg ctgcaggggg ctacggtaac
     9061 cctggcgggt ggtgagtatc cctttgacac gctggtgcaa cagaccgata cgggaggtat
     9121 agcctcattt tcggatatta tcaagggagt ttatgagatg agtatcacaa gccctggatt
     9181 tgaaacgtac aacgttttct acaacattca gtcacattct accatggaga taattctcca
     9241 ggtgaatccg gcaaagcctg agaacctttc ggttgacggg gcaacgctac tggcaacctg
     9301 ggagcctcct cagtttgatc cgcccggcag catcgccgac acttcggtat tgcagggtta
     9361 tgcagtgtac cttgacggca ccttgcaggg acaaacccag gagttgtact gggatttttc
     9421 gcctgcctgc ctcaacaccg gtcagagtta tactgccgca gtagcaggcg tatactgcac
     9481 cggaatcggc gaagccgata ctttcatatt tacgagcagt tatttacccc cgccacaaaa
     9541 tctgacagct tcggcaacag catcagtcgg tatcggtacg gcaactttct cctggcaaat
     9601 gccggatacg tcatgtacgg cctgcgatcc ggtttcattt atgatctata atttcggaaa
     9661 cctcataggt gaaatcccgg ctgaaaatct atcatatacc atcccctccg ccagccccac
     9721 cacagaatgc ctggcggttt cggctaaata tgatctgagt tgcatgggtt ttccgggtca
     9781 attcggggaa tcagccataa cggaagaggt atgtattgat attacgctgg ggaatgacat
     9841 cccttttagt gaagactggg gctcaggatt gtttgaaacg aacctgtgga cgcctgggca
     9901 aaactgggtg attgccggaa ccggcgacag cccgtcaccg gcagcacggt ttgtttcaga
     9961 acccaatctg tcaggctatt ccagcgccct gcaaagtcac tggatcaacg ccctgctcac
    10021 cgacaccgtg atgccatatg acatttatct gtattacgac ctgaagctgc agaaggtggc
    10081 cgcctcttca acggaaaaac tggcggttga agttttcggc gctgacggct ggacgagggt
    10141 gaaggagtat gtgaacagcg aaaaccttga ccttgcgcag gaacggctgc tcatcagcca
    10201 gcaggcgaag aatcgcatat tcatgatcag gttcgtggcc gaaggcgaaa atacatccaa
    10261 tatttcctgg tgggaaacgg ataatatctc cgtaaaagcc gagctgaccg gtgttgcacc
    10321 tgccaatctc acagcttcgc catatgggaa cgatggagac gatatcttgc tgcaatggca
    10381 gccaccagcc gcaggaagtt acgctgcagg gcgacagggc aacaatgccg gtttctttga
    10441 gcccggcagc agcgaagagg ttgcaacaat tgctccggct gaattgctgg gctataacat
    10501 ttaccgccgg gctttctatt ctcccaatcc tggcccgggg ccaggttcag gattgtggtc
    10561 gctccttgca tcaacagttt ccggcgatac cctctacatt gacgaaaacc tgccgaacct
    10621 ggaaaccaat tgctatgagt actatgtcac ttcgttcttc agccagggag aatcgtcctc
    10681 ctcgaacacc gacagcgaat gcatctgcgt cggcatgaaa gaaaacacgg ctgaagtccc
    10741 tcttctctat ccgaatcctt ccgacaaatg ggtaaatatt atcctgccgg aaagatgcaa
    10801 gaccctgagc ctgtttaata ttgccggaaa agaacttaaa aaaattgata caaacggaaa
    10861 gcaggaaata acgctggata taaaaggatt tccgtcaggg gcctatttgg tccggtttgg
    10921 attaagcgac ggagagcgca tcacacaaaa gttgctgatc aggcattaac ggatcatctc
    10981 gtttataaaa ccgtccctga tttgcagaca ggtaaaaccg tcaggaaatg aagctctttc
    11041 agtaaaggat gacagtcaac agattaaaag ggaaagttta acctgatttt aattcttctt
    11101 tatttaacct ttaaatatca agccatgaaa acttcaatcc cgttacttgc cgttttcgcc
    11161 tttatggcta tggcaagttt ttattcttgc gaaaaatcgt ccgattccgg cgatgtaact
    11221 aaagacatca ttatcaataa aaccagtgat tcggtcctta ttttcgggag taattccgac
    11281 agcctgattc tcactccatg ggatcttgtt cttcagggat attaccacac cgacgaatcg
    11341 gctgaattgg atattgacgg tgatagcata gccgatatca ggttggtcag ttcaatcgaa
    11401 ggccttgccg gcactgctcc gataccggca tcgtatatta agtgcctgca ttcaaatgtc
    11461 atgcttcgca accgccataa gtccgacact ataacaatga aagaaacctg cgatacggta
    11521 tacgataatg gccggatgat catccaaacc gtccgcactt tcgaatgcaa cacttatctg
    11581 ccgggcgatt ccatcaccga agtgcgggag ataaaccata tagtttacaa cataggaggt
    11641 gattctgttg caacatacga tttatttgaa agtggttcat atacccttgc aagcagccct
    11701 tacaccacgc catggatgac aggtttccag agcgacagcc tcgtcatcac aagcatgggg
    11761 gtgtataatg ataattgttg caccatgcct gatgatgtcc ccgtttatat cgggtttaaa
    11821 ataattacgg acggcaagcc gcgcctggga tggataaaac tgagcgtgca cgattattcg
    11881 atcattcata tttatgagac ggctatacag ccggtgagcg cggtgaagtg aggatcagaa
    11941 aaaagccgta tcaagcttcg gtaaggcgga agtcaatgcc tttaaaaacc tctgtcgggg
    12001 gcgatagcgg gttgatttcc tgtttcggaa acgcagccct ggcagaggaa tattcattat
    12061 tcaggctatc caagctttcc atatctttgc cgcactaaca tttacgaaag aatgcgaacc
    12121 ctcatcatca aactgttcag cgttgctgtc atcctgatgg cagtcaataa caggatatcc
    12181 gcccagcaac aggatatggc atggccgggc ccgaaaggcg tgtttatttt tacaggagat
    12241 aagataccca caggacaggt catatatgcc taccaggtgg aacggacggg gcctgacagc
    12301 gtctgggtta agatcggtgt actgagaaca ccggctacct tccagcagtt cctggataag
    12361 gttaacgaca cgaaaaagat atttcccgat cagccgttgc ctgcatcggc aaaactcgaa
    12421 caattattta agaaagccct tgcgacaggg actgtcgatt cgctgacggg ctggaagaac
    12481 taccctgtcc gtatggcttt gggtgtgatg tattatgatg accatgctgt gaatggcaaa
    12541 ttccggtacc gcatttctga agtggataca gcccgcaagg tggtttcctc ggtgatcaca
    12601 aatgccgttt cgatgcccca cacggcgaaa ttcgatccca tcatggttga tgaaagttca
    12661 aggacggctc atctcaacta tatccggtgg tattcggcgg ggaacaatcc tgcgcccctg
    12721 tttcagcctt ttcgtttcga aaacgggaag aatattgcca tcagcggctg gacgtccaga
    12781 aacagcgtga atgatacaag ttattacacg ttcaccgatt ctgttccggg agccataaaa
    12841 gccgagcaat tcttcatcgt tccgttcgat cctttcggaa atgccggtgc agcatccgaa
    12901 atattaaaaa ccggtcccga caggttcaca aattcaaaga tcaataaggt aaaagccggc
    12961 cgcatccccg acaaatacgg catccgtgtg tcatggtttt ataatgcgcc aaaacaaatc
    13021 aaaaacttta ttatctaccg cagcgagaat aaagacaaag gattcaggga atacacaagg
    13081 gtaccttcgg ccgacaccgt gtattgcgat gttaatgcct ggcccggcca tacttattac
    13141 tattatctaa aagcatacga cactaccggg aagcggttca ttacgagtga gaaagcatgg
    13201 tatacggcca gtctggcttc gaaaccgggc gctccaaagg ttacggcgga agctgtaaag
    13261 ggaggcgtta agttgacggt tgacgtgaat gatgacaaag ctgacggagt gcgtattttc
    13321 aggaatgacg ggctttcgga acagatgatc gccatcacgc aactgatacc gttaaacgga
    13381 aaacaaacca ttgaatatac cgattcctcc gaaaatcttt cgggacggca taattactgt
    13441 tatcaggtgc gttccgaagc gaaggatgca ggcgtcagcg aattatcgga aaaagctacg
    13501 gcaaagcccc ttatcaatac gccgcctgcc gatccggcat ttatccgcgg atcagtggcc
    13561 gataacaaga taaacctgct gtggcaggaa gctggttcag ccgattcctc gataaccggt
    13621 tataaactta cccgccggga gatgaaaaat ttcccggaca atggtcaggc ggaaggacat
    13681 atcagttcaa gtccggttac cgtcggtatc aatacctggt cggataccct tacaaaaccc
    13741 ggatatattt attcatacat cgttcagagc cgcgataagg aagaaatgat gagtcccggc
    13801 gctgcccgta tcattatttc gctggccaag gactccccca taccgcccga ttatatcaaa
    13861 gcaaaagaaa tatggatcgg taaaccggcc atcatgattg aatgggccga ggtaatgtat
    13921 gaaggtttta ctcagatcaa acttttcagg caatcggaaa acaaagaagc cgagctcatt
    13981 gcaaccttaa agcccggaaa tacgcattat gtggataaga ttgagggaaa gccggagacg
    14041 tactcctatt tcatcaaaac ggtgcattca tcagggacgg agagcaaacc ttcggccaca
    14101 gccaaatccg gaaaatgacg acccccagga ttaaggtcct ccttttctca taccaaggta
    14161 ttgggtttcc tgtcggtcat taaaatagta catgacaggc gcttctatct cgttccggat
    14221 tcctggttaa aatccgttga ggttcatggt aacggtcggg attagtaata tccagccaag
    14281 gataacaagg atcaatatca gcttccaggc ccagctaaac catttgttaa agggtattct
    14341 tgccacttca aggacgccga tcagcacgcc ggaagtaggc gtaataaggt ttgtaaaccc
    14401 gtcaccgata tgaaacgccg taacggtagc ctgttttgag aggttcacca tttcggatat
    14461 gtccctcagg atgggcatga taagggccgc tttcgcggtg ccggaagtaa tgatcatgtt
    14521 gaggcccgac tggaagacat acatcagcga aagcgttgta atcttgcctg ttccttcgat
    14581 ggctcttgag attccgtaca ggattgtgtc gataatcctg ccatcttcca gaacgacgat
    14641 aattccgccg gccagtccca cgaccagcgc tgccgacata atatccctgg caccggctac
    14701 gaacgaacgg gtgatctcgt tggcatcctt gcccatggca atgcctgcca ccagggccat
    14761 ggcaaaaaac agggctgcca gttccagcag gccccattca aaacctgtaa cccctacaac
    14821 caggaacaga atggtaaaaa tgagtatgtc aaggacaaac gcctgaaccg atctgcgtgc
    14881 agccaagaaa ccggtaacag caaacaaggc tgccagcagg ggaagcgccg ggaacaggaa
    14941 tgaattggcc cccagcctga tttcgttcat cggccagatg aaggccgaaa ccataatggc
    15001 ggctaacaca ccggcaaaag caacccaggt tgaaggaagg gatttgacgg aaacctgcat
    15061 gtcggccccg gtatgatgtt cgcgccagta actgtcctcc ttgtaaacaa cagacctggc
    15121 cgggtttttc tttacccggc cggcatacca caatacataa cttatcccca caatattgat
    15181 aaccatccag atgacgaaac ggtattcaag tcccgaaaag agctgaagcc ctgaaatttc
    15241 ctgggcaatt cccagcgtga acggattcat caggcagcct gcaaaaccaa tgccggcccc
    15301 gataaaacac atacaaaccc caacaatgga atcatacccc atcgaaatag ccatgggcac
    15361 aaaaatgatt acaaaagcga tggtctcctc gctcataccg aaaacagccc cgaaaaagct
    15421 aaacatgagc atcacaaccg taatgataat attgttaacg cccagaaacc gtataaacct
    15481 gtaccggttc agtttgtcgc ttttcttcag gaaagcgtat actcccatgt cgatggcctt
    15541 gctctcgttc agtatccaga aagcgccgcc caggatcagg atgaatacga ttattttcgg
    15601 tgagcggaca aatcctttat agaaagctga aaatatctgc caggtctgag gggctgattc
    15661 aacataatgg aacgagttgg tcctgatcac ttcttttgtc cctgaagccg tggttatggt
    15721 ttcgcggtcg aatgcgcctc ccggcacaaa ccatgtaaaa acggcggcaa taacaataat
    15781 gataaatacg atgacgtaag tgtgcggaat cttgagcctt ctggacatgt cgggatacag
    15841 gttgtttata tgatccttca aaagtatgaa aatacattgt tcatcatttc tttcccgtgt
    15901 gagcatgttc ctcagggggc tgtggaaacc gttccgtatt taatatccgg cattcttaac
    15961 tatctttgtc cggtagaaaa acacccattg tttaactcaa atccttccca ttgtacgttt
    16021 tccgacgcat tctcagagca ttccggttgc tgctgaaata ttccctgctt cttctattaa
    16081 caattctgct tctgcctctt tttaattaca tcgaaccggc aaggtatgat tttcctgctt
    16141 cagaggcttt caaagggagt ttttggtata atccttatca ggggatggac agcgcgcaat
    16201 ggcttcctgc caattttcac atgcattccc gggcttgggg cggactgacg aacggcgccg
    16261 ataccagaga ccatgaggtg tacgatacct ataaaaaaat tggctttggc agtatcgcca
    16321 tcagcaacta ccagaaaata aatgctttat acagcgacaa agccttctat atcccggctt
    16381 atgaacatgg atacggcatc tataaaaacc accatctctg cctgggggca aaaaaggtag
    16441 tatggtatga cctcccttac ggccagaata ttcatcataa acaatacatc ctcaacaggc
    16501 tgaggccaac cacagatata ctctccatca atcatccgtc atttttcaag ggatacatgc
    16561 ccgaagattt cacccggcta acgggatatg acggtataga agtcctgaac ggttaccgca
    16621 actctattcc tcactgggat tcagcgcttt cggccgggag gcctgcgttt atctttgccg
    16681 atgacgatat gcacgacatt tcggttccgg atgaaatcgg gcggcgcttc ctcgttatta
    16741 actcaccttc aaacagtcgg tccgatatcc tcagcgccct gaaatccggc aaagcttacg
    16801 gggtttatta ccagggagat aaaggggaaa gtctgactga aaaatcggag aagatcaacc
    16861 atttacctgt catcacaacc tttgatgtga agaacgatac ccttagcctg accatcgatt
    16921 caacagctta tgaagtccgc ttttacggcc agcacggcag attattgaaa aaaaaaggcg
    16981 gtatccggaa ggccttttat gctttacagg aagacgatag ctatgtaagg gcggtaatcc
    17041 tgttccctac aaggagcagc cacgaagggc tccagtattt tttaaatcct gtgctccgca
    17101 gcaaggatgg caaaaaacct cccatgcctc aggcagaaat aaaccaaacg ggaacctggt
    17161 tatcgaggat tggcctttcg ttagtggctt tgacatttat ctggctatcg gccagaatta
    17221 taaaatggcg taaggaaaag cgaaaatctt aatattacgg aagttgccgg aaatgatgca
    17281 acttgtatta aatgccggac tcaaaaaaaa taaaacgata catcctcctg ttgctggcgt
    17341 tttcggcggc gataagggct gtgctcgcag catcctttgc tctgggcaac gatgaagtgt
    17401 attactggac ttatgccctt tatccttctt tgagctattt cgaccatccc ccgatggtgg
    17461 catggcttat caggtttttc actgctaacc tgaccctgaa ccaggaattt ttcgtcaggc
    17521 tggcatccat cgttgcagga acccttaaca ccttgctgat cttcagcctc ggcaggaagc
    17581 tgaaggacga gcttaccggc tggtacgccg cattgctata cacggcttcc gtttacggat
    17641 ttatcatcac gggaacattt atactcccgg acactccgca aactgttttc tggcttgctg
    17701 ccctgaggct catgacggat gtgctccccg atcatctgct caccaccgga agccgacgga
    17761 agatgcttct caccggaatt ttgcttggcg ccggaatgct ttcaaaatat tcgacagctt
    17821 tcctgtggct gggaatgatg atttttatcc ttgtaaaaaa ccgcagatgg ctcgccgcct
    17881 ggcagttcta tgctgcaaat atcctaatcc tccttctttt cgcacctgtc atcctttgga
    17941 acgccgataa ctcctttatc agtttttcgt ttcacagcca gcgtatcgct ctttccgaac
    18001 atctgatcaa tttcaactcc ttcgttacgg aactgggagg cgagatgctc tattccaacc
    18061 cggtaaatgt gatattgttc atcctggcgc tgatcgccat actcaggatg ccggcacgca
    18121 gaagaaatga cggtgtaagg ctgctattat ggtcaggcct gccgctgatc cttgttttct
    18181 ggatcgtttc cctgttcagg agcaccctgc cgcattggtc cgggcccgga tacgtgagcc
    18241 tgatcccggt tacagccttc tggcttcgct cgttacatga aaatcttttc ccgaaagcca
    18301 taaaaacttc attggcagca gccggtgttg ttcttaccgt aagccttata cagattacta
    18361 ccggcatcat tccgtggcct gccggcagcg attcatatcc ggaaaaaggc gcagacgata
    18421 tttcccttga attaacggga tggcgcacgc tgggtaaaga attcacggaa ttatcaggga
    18481 aatatgaaaa atccggcctg atgcctgcag gggctcccat cataagctac cgttggttcc
    18541 cggccgctaa tctggaatac tatgccgccc ggccttcaaa acgatttttg ctggccgccg
    18601 gcccgttaaa ttccatccac cagtatgcct ggacaaatac ccttcacggc ggttttaaac
    18661 tgaacaccga tgcctggtac atcacttcca gccgagattt tcgtccgccg gcagcgttaa
    18721 aacctttata cttcagccac acttctgatc ccgacaccat aacggtgaca agatgcggca
    18781 aagcggcata ttatttcttc gtttaccgaa tgaaggatat gcaggcaaaa ccggcagcaa
    18841 tcgtagagta gacctcctgc ttgccgggta aatcattcaa aaacccggaa actctcctct
    18901 tccggacaaa gagcaaagct aatcaacaat taatttttta accgttgccg gaaaatccgc
    18961 gctttcaagt ttgacgaggt aaaccccttt tggccagcca tgtacgttga ctttgcaaat
    19021 tcccgtacat gaatttgccg gcaatgacga catcagcgat ccgagctgcg aataaaccag
    19081 gatttgcgct tcacatcttt ccggaataac ataaggtata ttgacttcct ggtcggcagg
    19141 attaggaaac ggggttccca acccgccggc tacagcttca tccttgtctt caactttagt
    19201 gggatcaagc aggatgctta ctttaaaatc gtcgaaataa tagccgtcgg cttgaacgcc
    19261 cccatccgac cggagggtaa accggaataa tacattttga tcaagaaaat cgttcaggta
    19321 aatggtttct tttacccatt ccgactgaat gccgtcatag agaggttcgc ctgccgcctg
    19381 gtttatcgta cccggacggg tatattgacc ggtaagcgga atccatgtta ggccatcatc
    19441 ggttgaggct tttacctgta catagtcgta tcctgcttcg aggttccact gcgcctcaaa
    19501 ttcaagcatg gcaaacatag cattctgcaa ggataccggt actatggtgg ttgtactgcg
    19561 gttggcattg ttggagtaat ttcccgaagg tgaatcggtc atcgatttgg aaggagaaac
    19621 atatttcagg gaagtcaatg cccattggcc agtccagtta ccggtagtat taaggctgtc
    19681 gttcacaatt gcaaccggca tcccgtaaaa tttctctatt gtatcggcta tggagaagga
    19741 accgttatca aggatcaggg catacctgat ggtatcgcct gacataatca acggatcaag
    19801 cgtataggcg atagaatcgt cgatggattg cagcaactgc atgccggtga atatcttagc
    19861 atcgccgaca gatgctatgg aactgcctaa aggcgcaacc gtcacggtaa aattgccatt
    19921 ggtaaggcca tatcttttga tgttgaaact gaaatatcct gaaggtttac tgacaaacac
    19981 cggcgaatta tctttaacga tggcatagtt accaactaac tgcgcggcgc gaaggctttg
    20041 cagcatattg atctggcaca gcgggatgat acgcgactgc atgggccaga aaccatcgtt
    20101 gtcgcctcct acttccggcg tccaggccag cattttgttt ttggtgcttt gctctccata
    20161 catccagtcg tcggaacctc cgtttgtaat atagatcgta gtactgctcg ggccgtaaat
    20221 gaagttgttc tcttccgtca tgattcggga gagattattg aagagggatt catccggggt
    20281 aacgtcttcc gtgtatcccc aagcatagag aaatttgttg gcatatgaat gatagttaat
    20341 tgcaatacca aaatcatgtg cttcgcagaa gtatttgatc atccgcgttt ccggttccga
    20401 gaatgctgct gtgccgcgga agcattcatt gcccggatca ggcgatgatc cctgattgtc
    20461 attcccccac atgtacccgt aattacggtt tatatcaatg ccaaaacttc catcctgatt
    20521 caggcggcgg ttcttgcgcc acatacctcc gcctccggga ttggaagaaa tattataggc
    20581 ataaccgtct acgttcacaa ccgggataaa atacatttcg gtggtatcta ccaacgcttt
    20641 gatcatcggg tcggaatcat agttctcgag caggtaccac atataaaaaa gcaggtgttg
    20701 catggcaatc ggctcacggg catgatgcat gccggtatac agcacttccg gttcttcctc
    20761 gtcaatgccc ggattgtcgc ttatttttac ataatacatc acctggccat tgcaggtagc
    20821 agaggtgtcg ctgacagcca tcctggccgt gatcagtccg ggataaaggg tgtgcatctc
    20881 atccagttgt tcaagcatct ggtcaatggt cgaaaatccg ccgcaactgc ccagttggaa
    20941 atttgccgga acaggccatt cctcagagac cgccctatcc gtttctgttg tttcataatt
    21001 ttcgggatgc acgtaccggt cggcatacca ggcttccaaa tcgggaatga ggacctcgca
    21061 ggaaaatccc aactgtgaaa ccctggccag ttcttcgtcc gacaactcag ctatagccca
    21121 gtctccattc ttaccgatga cctgaacctc aattccggcc tgataaagcg cccgttgatc
    21181 atggtcgcca aaaaaaattt tcactcttga atagttccca tccgttgcaa agagaaagca
    21241 cgggacccaa agcgctgcca gcaccagaag aatatgtttc atagtgattt gtttattagt
    21301 agccaaagct aagaaaaaaa aataatccgg catgcaaagg ataaaatgcc ggaattacaa
    21361 aacagaagaa tcgtttgttt atttactatt tcaccccgga tgaggctcgg ttcttccacc
    21421 tgaaagtgct gatcagatgg gcaatatcct gtttaacgaa gtcaatgacc ggcgacaggg
    21481 aatcattatt cggaaccgta gtgaaataca atgctccccg gaggaaattc cgggtgctgt
    21541 cggtgaggta aaactggtag ggcgatgctg cacctacccc gcttatttca taacagaccc
    21601 cataaacgtt attctcttta tcccggatct ctttttgaaa aatcccgctt gattttggaa
    21661 tgtgtttgag gactaatgac cgtgaatcct ctgttagttg ctgaaggtta ttttcgatag
    21721 tgcggtaact gatatgaatt gtccccttaa actgaggata atcaatattg atccaatata
    21781 tttctttggg cgacatctca tcgggatgga ttcgggcata gacgggatat tcaaaagtgt
    21841 agggatagat ggaatcgaga agggtgtact ttttctctgg aagggcaata cggaaatagc
    21901 ccctgggttt aggagaataa tccccggaac acgaaatgag taccgttaat ataacggcgc
    21961 aggggaataa tatcataaac ttgctcttaa ggcgtaacat atggcaattg ctgttaattg
    22021 ttattcaatg gtttgatttc agcgccaacc acaggaccag gtctggcggg cggtgttgtg
    22081 tgctgttggc tattgttttc gggcatccgg attcaggatg ctgcattcct gaactcttcc
    22141 tttttcactg ttggcctact cttccccggc atccggttca ctgagggtca ctctcaccct
    22201 ttttatcctt cttttatcgg ctgcttcaat cctgaaagtg aaatttccgt aagttatccg
    22261 ttcgttagtc cggggaattt taccaaataa ttccagcaat aatcccgcaa gcgtgtcgct
    22321 ttcgcctttg gcttcttcaa aaaccctgtc actgatttcc agtattttac agaaatcgtt
    22381 taattgggtt ttaccatcaa agataaaatt gtgatcatca atcctggtgt atcccggttc
    22441 gtcactctcc gaatcgaatt catcgctgat ttcacccaca atttcttcaa taatatcttc
    22501 catagtgact attcctgaag ttccgccata ttcgtcaacc acaacggcca gatgtatttt
    22561 ctttgcctgg aattcttcaa gcaggtcgtc aatgggtttg ttttcgggga cgaagaatgc
    22621 atttcttacc agtttggtcc agccgaaatc gtcgtcacgt gcaatatgcg gcaacagatc
    22681 cttgatatac aacactccgg ttaccttatc cggcgtttca tcataggtcg gaattcttga
    22741 atagcctgat tcaacaatta cttttttaac ctggctgaag ggttctgaaa tttcaagcgc
    22801 ggtaacatca atgcggggcc gcatgatctc gcgtactgta atatccccga aacgggtaat
    22861 gcttttcagg attttccggt cgtcttcgtc ggttgccccg tcagcagtta tatcaatggc
    22921 atccgacaac tcttccatgc tgatcggata ctttttacgt tcaatccttt tatcgataat
    22981 attcgttgaa cctatcagca ggacactcag cggatgaaac aagcggacgg tgaatgccag
    23041 gggcctggcc ataaatttgg ccaggcggac agggttcatg gtagcatata ccttagggag
    23101 catttctcct accagcagga taagcgaagt aattacaaca acctgaatga taaagcccaa
    23161 aacaggaaaa gcggagaaat cgagaaattt tcccgtgaaa taggtcgaaa tcattacaat
    23221 ggccacatta acaaagttat tggcaatgag gatagtggcc agcaggcgct ttggccggtt
    23281 gagcagtttg ataatcaatt ggttgatagt ccccttcttt gattgcagtt catttagttg
    23341 tgactgtgac agggagaaaa aggctatttc gctgcccgac atcagggccg agcacattag
    23401 cagcgcaatc agcaaagctg ccaacacgat gatattcagc acttcctgta ctccggaagg
    23461 ctgaatcatc agtatcagga aaagcaaatt tgaataaccg gtaaaaaggt aatccacgtt
    23521 atgggttcaa attgataagc tttacaaaga tacaaaagag gatgatcata caggccatgg
    23581 ccccgggact gcaatattac ctgccattgc catattaatc aggaaaatgg ttgccgacat
    23641 atttttctat caggcgcgga agtggcaatt cgcggatgtc ctgtatcctg acataccggt
    23701 accgtttcat ggatggggtt tcgtaatctg catgaatcct gaaaaaacgg gctatgatct
    23761 ttctatgggt gaggagatgt gcaaccggac cggcaacatg cagcagcgtc cagtcattac
    23821 caagcaattc acgggcttct gccgatgcag ccaactcttc cggattgatc agccggtcgt
    23881 tttcaacaga gggaaaatca tacagattat gccatatatc cctgccggtg cgtttgttca
    23941 gtaagatcgt ttcgacgcca tttcctgtga aggtaataac cagatagttg aaatagcgta
    24001 tgcttacgac tggttttgat ggtttcacag gtaattgctc aaccatttgc cggtcgaaag
    24061 cataacaatg taactgcaac gggcattccc ggcagcccgg cgaaagcggt gtgcattgca
    24121 gcgcgccaaa ttccatcaca gcctggttga agagccccgg cgggtgattg cccatcagct
    24181 ctttcatggt atcattaatc aggtggtaca cggaaggcga cctggcatcc ccggttacgc
    24241 cgaataatct tgaaattacc cttatcacat tgccgtctat aaccggtaca gcttcaccga
    24301 aagcaattga ggcgatggcc gcagccgtat atttgccaat tccgggcagt tttacaagtt
    24361 cggccgaggt ggagggaaat tggccaaggt atttttccac caccagtttt gatgttttat
    24421 acaggttgcg ggcgcgtgaa taataaccca atccctgcca gcattttaag acttcgggtt
    24481 cagaagcccg cgccagatga aaaaccgtag gatattcccc ggcaaagcgg ttataatagg
    24541 acaggccttg gctgacgcgc gtttgctgca gaatgatttc cgaaagccac accagatatg
    24601 gatcgcggga agatcgccag ggcaggtcgc gcctgttatc ggcataccat tctaaaaata
    24661 tatcagccaa ccccctgttc atggcttcaa aaatacagca attcccggtt cctgaaattg
    24721 aatttcaggc catccgccac ggttgggtat ggaaatatta agaggcctgg agataagccg
    24781 gttgaggaca atattttgaa aatttatttc gtatttagtt ggtaaaacaa aaagttccta
    24841 tatttgcagc ccgtataaaa aatttagtat caattcatta aaccatacgt agcaatgaca
    24901 aaagcagaaa tcgtagctga aattgcaaac aagaccaaca ttgagaaatt ggctgtgcaa
    24961 cagacagttg aagcatttat ggatgcaata aaggatgcaa tggcacacgg tgagaacgtg
    25021 tatctgaggg gctttggaag cttcatcgta aagaaaaggg ctgaaaaaac cggtaggaac
    25081 atttcgaaaa acacaacgat cattatcccc gcacacaata tcccttcgtt taaaccagct
    25141 aaggtttttg tgaatgaagt aaaagtaaaa gtaaaataac aaaaattcat ttaaaaatca
    25201 catatcatgc caagcggaaa aaagagaaaa cgccataaaa tgtcgacgca caagcgtaaa
    25261 aaacgcttgc gcaagaacag acataagaag aaataattct ccattcaacc aatgaatttc
    25321 atcacatgaa atggatcatt atttcgtgtg atgaaatttt tatttctgtc aataacatct
    25381 gacatcgaca gcaatttatt attaactttg aggccatgaa tcggctttta ctccaagact
    25441 atccccggtg aataaggaac tgataattaa tgccggcccg aatgaagtga acattgcctt
    25501 actcgaggat aaggttctgg tagaacttaa taaggaaaaa acaaacaaca attttgcggt
    25561 aggagatgtt tacctgggaa gggtcaagaa aattatgccc gggctcaatg ccgcgtttgt
    25621 agatgtaggc tacgaaaaag atgccttcct gcattacctc gatctcggtc cgcagatcaa
    25681 ttcgcttata aaatttacca ggatgggtat gtcaggcaac acggcacact tgtctatgag
    25741 cgaatttgaa acggctcccg acatcgaaaa gaccggaaaa atcggtcagg tgctttcttc
    25801 aaaccaacag attctggttc agattgccaa agagcccatt tcaacaaaag gcccccgtat
    25861 ctcttccgaa atttcatttg cgggacggta cctggtgctg gtcccctttt cgaaccgcat
    25921 ctcaatatcg cagaagatta aaagcgccga agaacgcaac cggctgaaaa ggctcatcat
    25981 gagcatcagg ccgaaaaatt acggcgttat catccggacg gttgcagaga acaaaaaagt
    26041 cattgacctc gacaacgacc ttaacagcct ggttcaaaga tgggaatcca ttgccgctaa
    26101 gctgccatcc gccaaacctc cccagaaaat actcagtgaa ctggatcgca catcggccat
    26161 cctccgcgat atgctcagtg attcgttcaa caatattcat gttgacgaca gcgtccttta
    26221 tgaggaagta cgcaactaca tccgcaccat cgcccccgac caggttgata tcgtaaaact
    26281 gtacaagggc aaaatacccg tatttgaaac tttcggcgtc gaaaaacaga taaaaaattc
    26341 cttcggaaag gtcatcacca ttaagagcgg ggtttatctg attattgaac acaccgaagc
    26401 ccttcatgtg attgatgtga acagcgggca caaagttaac agcgagataa gccaggagga
    26461 gaatgcactt gccgtgaaca ttgaagccgc catagagatt gcccggcaac tgcgccttcg
    26521 cgatatgggc ggcatcatcg ttatcgactt cattgatatg cacgacgggc gcaaccgacg
    26581 tacgctttac cagaagctga aagacgaaat ggccaaggat catgccaagc acagcatcct
    26641 gccgccaagc aagttcgggc tggtacaaat cacccgtcaa cgtgttcgtc ccgaaaccac
    26701 agttcaggta ctggaaaaat gccctgcctg tgaagggacc ggtgaaatca aacccagcat
    26761 tatcattccc gattatatcg aaaacaacat ccgctatttg ctgcaggacc agaacgagca
    26821 gtatctgaaa gtcactgttc acccatattt gtacgcctac ctgcgacagg gcggactatt
    26881 ccgttccatt cagtggaaat ggttccttac ttataaaaaa tggatcaggc tgatgccggt
    26941 tacggcacaa catctcctgg aatatcattt cttcaacagg aatcaggacg agattaaact
    27001 ttaaattcgt caatccggct cctgctgtgc aacggctcac ccgaagtttt aatccgggaa
    27061 acaccgggaa gtcaaacagg aaacaattaa tatgaaagca agccggtatt tggattcggc
    27121 aagggttaat tcatcctgtt gtgaaccgta ttttcaacac tgatcagaag gtcatcgcga
    27181 ttaatcggtt ttttgaggaa ttcctggcat ccgctcagga aagcatcctc ttcactcgta
    27241 aacgaaacat aagccgtcag gacaataacc ggtaaatcag gcaggaaacc cttgatttgc
    27301 ctgattgctt ccctgccgtc catcagcggc atgttcagat ccattaatac caggcttact
    27361 tcgggattcg aatggcagat ttccaccgct tcgaggccat tgacagccct tattaccctg
    27421 tacccggcat tgttaaggac tgcttcgata tacatatagt tgaagtcatt atcttcggcc
    27481 acaagaataa ccgggttctc tttaaccgga atctttgccg ggggcgcttt ttccgatact
    27541 acagcagtag ccatcgctcc ggccggcaaa gtaaaataaa accgcgagcc tttgcccggt
    27601 gttgattcta aatgaatttt cccttcaaga atggttacca ggccgtgaac aatagataat
    27661 ccgatattcc cgcctgtaaa gtcatagggc gccgtggtat cggccaggtt gaaataatcg
    27721 ttgattgaat ttacctgatc gctgtccata cccgtaccgg aatcggctac aaaaaatccc
    27781 ggcgtgtttt tactcaacct gaatccgaca tcaatggaac cttcttgggt gaacttaact
    27841 gcattattca ccaggtgggt gaaaattttg tgcagaattt ccggattggt attcaaaacg
    27901 atagcatcag gagctgccgg ttgtataata ttcagggtaa tacctccggc ttcacaaacg
    27961 cccttcattt cctttttcac cttttccaaa agggtgccga ctgtgcaatg ctttttatga
    28021 atggtacctt tgcctgacag gatcattgaa atatccaggt agctgttaat ggtattcaac
    28081 aggttcaggc tgctcttcga aacgatatcc ataaaattca tcttggtttc atctgagaaa
    28141 tcgggattct gcaacatctc ggtaaatccg ataatgccgg tgagcgggct ccggatttca
    28201 tctgaaatgt tattgataaa tcctgttttc atcttgtcgg tgatctcagc cctgactttt
    28261 gccgaagtaa gttcatccat cgttttatgc cattcatcga tattttcgtt acttaccaca
    28321 atatgaatga gttgcccgtg tttgttgttt atagggacaa tatgaacatt ttcccaggcc
    28381 ggctggccat tctcaccggt ggtggagcat tcccccgacc atgactgccc tgattgaacc
    28441 atattccaga tttcggtgaa tgtgttgccg gaattatttg cataatacct gaaccaaaga
    28501 tttttaccaa tgacatcggc aaggcttaaa ccggtaacag cggaatattt ctggtttata
    28561 tactcaatat ctcccaaagg gttggctacc aggagtccga ccggactgtt ttcagccata
    28621 atggtcatcc actgtgcctg ctggtcaatc gttttcatag aatttacatc ccggactgcc
    28681 gtccacaggt aattcttacc cattattatg actttaccag tagtgacttc tacatttttc
    28741 gttgttccgt tcttgcattt atgccaggtt tcataatgaa ggttccctgt agttttaaca
    28801 ctttcggtga ggttagccag ttcgtgcggc gtaatattga acaattccct cggatttatt
    28861 tttaaaaatt cttctgtact ccatccatag agctttgttg cagcaaggtt tgccgtcaca
    28921 attttttgct cctgaagatc gatgagcaat atggacgtat cctggttgtc gaatatgctg
    28981 cggtacattt cttcattcca tcggagagaa tttgcagctt caaccgcgcc tgtcgtatcc
    29041 ctgatggttc cgacgataag atcaggctgg ccggaagcat tatacaacaa acgcgcataa
    29101 atggatccgt gtttcatttc accggtcttt gtccggaacc tgatcttaaa atcgcgcatt
    29161 tcgccgtgtt tcaccagcga ctggatcaat gattccctgt cccgggcatc ccagtataga
    29221 gtaaataccg aattcccgat aagctcgctt ttttcatact taaggtagct ggcaattgaa
    29281 ggactgatgt cataaaggtc gcccatcatg tcggtttgaa aatatatatc gggtatatta
    29341 ttgaacaggg ttgtgaaaaa tgtttctctc cctgtcggaa ttcccgcgac attcgccgat
    29401 gccgtgttcc ttttggcata agcggcagtt tcgccccata tttcatttcc taatgcagag
    29461 gcgatgacct ggctcagccg gtttaactgt tttttgctgc aatgatttgt tgcccctgct
    29521 tccatgcatt cagtaaggag gttttccttc atcgccttgt aaaggattat taccggtgta
    29581 atcctggatt tgatccgggt aatccgcact atggaaaccg gatccaccga agcagcgtca
    29641 agggaacaga tgaccaggtg cggatttaat tcattcaact gacgcacaaa atcattcttt
    29701 gaatttaccg aattcacctg atatgattca aggacatttt caatctggtg ctttatcagg
    29761 cctatatctg cctgacggtc ttcaaccagt agtatgcggc aattttccat cggtttcaca
    29821 ttttagatta tgactggtgc attgatttta atccggaaga ggttcaaata tttcatccgg
    29881 gattgttacc gctgcccgga gacctttaat agctcttttg tttagtttaa ggataatcct
    29941 gctgttatta tgataaaagc catacaactg ccggaggatg atataaaaca ggcaattgcg
    30001 gatgccggca gaccgggaga agccagtggc tcccctttga acagaaaacc acagcgcccc
    30061 gtttcaggcg aagggtcggg cttaaaggcg gaatatttct ccggctgagt aaaagccgtt
    30121 ccgcgatatt acccatcagg ttatgtaaat cccggcgttc cggaattcac ggaagggaag
    30181 gcaattctgc agcgttgtat tctccaatcc gactcaggaa cgcatcatga agcagaattt
    30241 ccgttgcatc caccggcatg taaccggcta tatgcgatta cacgggtgga aatgggataa
    30301 ccgttaatgc agtaagagaa atttttccta gtcaggagcc cggtaactcc aattacaagc
    30361 tttccggtac gacgttcgcg gaatccggga tttttgatgt tgaggccttt tccctgagag
    30421 attgcaccca gcgttccatg gcggcctgac tgttattccg gaaacccggt atcacaaatt
    30481 tacgacataa ttgagcaata tgcatatcgt tcctcatctt accggcatgc ttacatgccg
    30541 agtttccgaa accatttctg ttgccctgaa gaattaaaaa aatcaagttt accggcactg
    30601 ttccggctaa aagtcaggtt tcattttggc ccggatctat catgacagcc agttgaaaat
    30661 ttgctacatc gtgccagagc ggggttctca actatcaagt tttcagttca ggaatctgat
    30721 tgaaaccttt taaaaatcac tttggacagt aacttttcag cgtatccaat ccatcttctt
    30781 ttctccggat taataaacaa atcagccttt caccgccttc acttcatagt ccatcccgat
    30841 gagcacatcg gccatcggtt tcagcgcctg caccatctgg tgttcctttt caagaacagc
    30901 catacggtac tcgttttgcg gttccttgcc gcggacaacc tgtgcttttt ttgtttcatg
    30961 gtaggtgagt tcaatgaaaa tgcgcagatc aacgtcttcg gttttaatgg cgtacaggcc
    31021 gtcaatcaca agcatatcga tggcggaata atcggtggta agctggtcaa cttgttcggt
    31081 aaccagatcc acacaggggc cggtagatat ttttccttcc ctgaattcct gaatattccg
    31141 gtaaatggta tcccagtcgt attccccgta accgaccacg ttctcaatcc cgtgctgctt
    31201 gcgccattcg gtgcgcagca aaggcagaat tctgtaataa ttatcgatat gcaacggttt
    31261 ggcaaaaatg ccatagctac gcaagccttt cgaaatcaca tgtgccagtt cggactttcc
    31321 tgatcctgac tcgccggaaa tcccgatgat cattttaggc ttgcgcccgg aaaggatttt
    31381 ctctatgatg atttctccgg ctttccggtg tttttcggtg attaacagca catctcccaa
    31441 cataggtaaa gatttaaagt gtaacgttta agggttaaac cggagggctc ccgccggcag
    31501 ttcatcgcct gtcgtttggg gctatttact aaaggcatcc ttcgatttca ggccaaaggt
    31561 ggtgggtata tctttccaca attcatactt tttaccctta agttctattt cggcttcctt
    31621 atcggtgtcg atggtaatgc tatcgtgaca aaccctgaaa cgatatgcca cccccctgaa
    31681 aacaaatccg aattccatca tttcccaggt ttcaggcaag gcaggagata tcttaagaat
    31741 ttcaccgccg aaatttattc cggcataggc cgtgatggcg atgagtacgg taccggccat
    31801 taccccggca tgaatgcctt cggccgtcgt cccgccctga atatcggtat aatcgcttgc
    31861 cagggcgtcg ttgtacaatt cccggctcaa ggcatgatta cccgccagcg ctgccagttt
    31921 cgagtgaaca accttgctga gggtggaacc gtgggatgtc ctcctgaaat aatattcaag
    31981 gttccgttgg agataatccg caggcagcga atacccgagc cttgaaaata acctgtcaac
    32041 cgtttcttta tccagattat agaaagtcat cagcgcatcg gcctgtttcg agactttgaa
    32101 agcatcgggc gacaaacctt ccgatttcag gatgcggtcc atacggtaga tattcccata
    32161 tttcgactta tagaaatccc agtccagttc tttcagatcg aaataacctt cgtactgggc
    32221 aataatccca tcttcattaa tgggtattac cagcttttga gaaatatcat cccagtgttt
    32281 cagttcttga tcttttaatc ctatggcggt ttttactgat gctgttccct cctcaccgat
    32341 ggctgcaaga atttctccgg ttttcgagaa catccaggct gccatgaggt tggtgtatgc
    32401 attattggta agaccacctt cagccgatcc gggatattgt tcatggaatt catcagggcc
    32461 catcactttt ctgatatcgt aacggcctga tttttcattc cattctgatt ttctttccca
    32521 gaaccggcat atttcgaaat acatttctgc cccgtatcgc ctcatgaaat cagcatccag
    32581 cgtgatggaa tagtattgcc agatgttata ggcgattgcc agcgagatat gccgctgcag
    32641 ggagctatga tccgggtccc attcgccgtt cagcggattg aggtgtatca cctgcgtttc
    32701 ttccctgcca tcggaaccgc tttgccacgg gaacatcgcc cccctgtaac cgtgctcttc
    32761 ggcataagcc cgggctttgc caagccggtt atagcgatac atcagcatgg cccgggccac
    32821 ttcgggctgc tgcagatcgt acaaaggaag gataaaaagt tcatcccaga aaacatggcc
    32881 ccggtaagct tcgccgtgca acccgcgtgc cgtgatgcca gcatcaatct tacggttgtt
    32941 gggcgacatg gaaaccatca ggtgatacag gtgcaaccgg atcagcttct gcgaaaaacg
    33001 gtctcccgtg attttcagat caattttatc ccagattcgg gcccaggcat ttgtgctttc
    33061 ttccagaagc gattcaaaat caggtgcatt ggtcaccgct ttgtgcgatt cggccatcgg
    33121 gtcggtaacg tcatcctttt ttgatgtata tacggcaacc ttttttacca gggtatagag
    33181 gttcccggct tcaacgtcaa aggaagtttc accggacacc ataccgggct gtatctcagg
    33241 attcagatgg tggttttggg gagcaggacc ggcataaaac cggcaaaccg acgatacggc
    33301 tatcttcact cccgattgta cggtttcgac cagcagttgc tggatattgc cttctgctct
    33361 ttgttcaact ggttttaaat gtttttgatt caggctctta tacctttcga ccccatcgtt
    33421 gatgatatct ccgtttatac cggtccggac agtcaacctg tctgaataat tgagcggaat
    33481 gatggtatag cgcaaggctg caagatgcct gtcgctcatg gaagcaaacc tgtcgctgat
    33541 gatcagcgtt tcgttgccaa attcgtcccg gatttcgaga tgccgggtga acaaaccgtt
    33601 tcgaaaatca aggcgacgtt taatgtaagt gatctcatcg tcatcagcat ttgtccattt
    33661 accgttaccg atcctgaagt tgaccggaag ccagttgggg atatttacaa aatcttcgtt
    33721 ttcaatattt ttccccgcaa cgggagtaat cagcctgtta tagagcccgg caatataggt
    33781 tccggggtaa tgcacattgc cggcggagga ttcttccatg gcgccccgcg tacccaggta
    33841 tccgttgccg acagtcagca aagtttcgcg tgttttttcc ttttcaggtt cgcaggtgtg
    33901 gtaggtaata ctccagttat cactggccag gccctccccg aaccagtcat cgatatcttc
    33961 tatttcgatt tcttccagat cgctcaccac aatgtcggcg ccgtttgcat aaagttcggc
    34021 ggggttgttt tcgcgggcaa tgccgataac aagcccgaaa tttcctttcc gtcctgctgc
    34081 aacacccgat acggcatcct caatcacaac gctgcgcgag gggctgcaaa acaaattgtt
    34141 gcatgccgtt acgaaaatgt cgggttccgg tttgcctttc aatccaagtt cggaagaaac
    34201 caccccgtcg acacgggttt caataagttc cagaagcctg gcggctttaa gcacggtgta
    34261 acagttttta ctcgaagagg caactccgac atggatgttg ttttcaagaa gggtgtgcat
    34321 gagttttaca gtggaatcgt acacttccac tccttcgcgt tcgagcacct cgttaaaagc
    34381 aacgtttttc ctgtttccaa ggccgcaaac cgtctccttt cccggttcat ccgacggatc
    34441 gccgaaaggg atttctattt ttcttgactg aaggaatgat tttacaccgt cataacgggg
    34501 tttaccatcg atgtagggaa ggtaatcatt cgcatgggta aactcactga atggctcgcc
    34561 aaatcgttgt tgacggcttt taaggtattc gtcgaacatc tgtttccagg catgtccgtg
    34621 taccagggct gtcttcgtta taacgccatc aaggtcaaaa atgacggatt caaatttcgg
    34681 ggttggcatt gtggttttat gagttgaaaa gtgcgccggc tgcatcggca agcctgtttg
    34741 ataagttgta agttcgagac ggcggaaata tgttgacttt ttcagcttcg gtattcagaa
    34801 gcccgatatt cgtatcccct aactttctat cgtaatccac ctgaagccaa gcggctcaat
    34861 ttcgccggat aagggtttgc caaaaagatc tttcccgctg gcggcatctt ccggcaaact
    34921 gagcggcaat gcaaggtttg tcatgttgtg gatgatgaaa agcttttcgt cgccgtactg
    34981 ccggtagaac gccagcatgt tcccggatat tgtatctttc atttcgataa accggagggt
    35041 ccctctgccg aaagctttaa agtgacggcg gacgccgagc atttgttttg tgggataata
    35101 cacccggctg ataaaactgt tgctatcgtt gagttgtgtt tccagttttc cccagtcgat
    35161 tttgccacgg gccaggaagc gggtatcatc tttaccggca agttttatca tttcagcata
    35221 gtactcttcg tcgttaagtt tgccatattc atcaccataa taaatcacag gtgttccggg
    35281 caaggtaagc atgatggaat aggcaaggtg tattttccgg ggattgcgct gcatcagttc
    35341 cgaaaggcgc gctgcaattc cttcgcccag ccggaaatcc catttcggat tataacagta
    35401 gttttcatgg ataaatttcc gttcttcctc agtaacatac acgagttcaa ggctcagttc
    35461 atcatggcaa cgcagaaaag taaaccattg tcctgaagga ggcagttcgg gcgttacagc
    35521 cgggccaaga atttcctcga ccggcttgcc gttttgcatg gcaatggctt taaagatgcg
    35581 cggcatcagg gggaagtgat aagcggcatg acattcgtca ccgtcgccca ggtattgtac
    35641 aaccttataa ggttgctggc acgcttcggc gagtaaaagc gttcccggtt taatataatc
    35701 caggacagcc ctgaaaaatt taaccacaac gtgagtaagc ggatggtttt cacaggcagt
    35761 gccttcttct ttccagatgt aagggatagc atcggccctg aatccgtcga cgccacggtt
    35821 ttgccagtaa agcaggtgcc ggcacatggc cagcagaact tccgggtttt tataattcag
    35881 atcgggctgg aagttgaaga accggtggaa aaagtactcc tcgccacatt tttcccagtt
    35941 cgaattttca attcctttaa aaatgatacg cgcatcttta tacttatcag ggctactgtt
    36001 ccagataaag aactgacgat acggattatc cggccctttc gatgcttcgg taaaccaggg
    36061 atgctggtcg gaaatatggt tcagggcaat gtcgaaaatc accctgatac cccgatgatg
    36121 cgcttccgtc agaaaaaggt caaaaacccc ggtccgcccc aaaggactgt taccggccag
    36181 gtcctgccgg atgttgtaat agtcgctgat atcgaagccg gcatcgcgca tgggagagtc
    36241 aaggaccggg aggagccaga ggcaattgac gccaagctcc tgaatgtaat ccagtttatc
    36301 aataagcccc tggaaatcat tgttaaaaag gtcgacataa accgaatata caatggcatc
    36361 tttgtaccag tcggtttccg gcttttcatt tttcccgcat tcgcggtatg catctgccga
    36421 atgcaggaaa ttattcagaa tagctgttga aactccggga tacagcctgt tccagtattg
    36481 agtgagaaca tcacaaaccg cttccataag aaattatgaa acaactttta aagacaagga
    36541 aggaaaccgg cggtttaaaa accgtctttt tgaaaaaaaa gggctgaaac cagcggtttc
    36601 agccctggtg taatgaattc aaaatcagtt tttaacgatg gtgcaacttc ctgattgttc
    36661 gttggcgcta aggtacaact taatagtgta gttgccggcc tcggaaatag ggagatttgc
    36721 accgccctgc gacaggctgc tttcagtacc tcccaggttc caatcccagg catcgttcat
    36781 tctgaatttc acttctccgg caacaaggtc ggtagtaata taccaatagt tgccggttgc
    36841 attgtagtcc attttcaggt caggggcatt ccatccgtta ggcgtggccg aacccaccaa
    36901 ccctatcatg aacgatttta tagtgtaatc catgttgaca gtgctggcgg aaattttata
    36961 ccagccttcg gttgaaacgc tgaagctgtc accgttttca accagaacac cgccggaacc
    37021 gccataaact ttgccggcat caggatcggt catggtaaaa ggcgtaccgg ccggcagctt
    37081 ggcaaaaccg gtatagttgc catcgccaag ctgagaataa atattctggc tcggcgtcgt
    37141 cgacaacacc agtttgggat atccataagg agtcaccgtc atggtcagtt ccggtgaatg
    37201 caggtcatca acacccggca acccgtaaga tgtcaccctg acttttatcg tggcactttc
    37261 gtctgcattc aatggtacgg agagcatctt ggcattgaaa tcgccgacgg taaccttgaa
    37321 gctgttagca tttgtggtag ttgcgacaac agtaggagag tcaaaactcc ctgttgcgga
    37381 aatcacctca agtttgtatg attcggcagc aacaaaacca tactcggcca gagcccctgt
    37441 gaaagagagt gtatctgatg cttcggtcct cttcaggaca atattgtccg atggcaatga
    37501 aagggtcgga gcggttgggg aggccgacag gaggattttt gtttcatctt tctcacagga
    37561 gattaaggtt gttaatccga ttattgtgag taatatccag attttgtttt tcataggtcc
    37621 tggtatttta attttaatag cccgggtttt gatttttgat attcggattg gccgtcatat
    37681 cggaagacgg aatcgggaag atgttgaaat gtttatctgt ttcgattcct tcggcgatac
    37741 cacccttcca gggccaaaca taatcgccgg aactgaacct gccaaaacgg acaaggtcgg
    37801 tacgacgggt gcattcccaa tagagttcac gggcacgttc atcaaggatg aaatcgaggg
    37861 taagctcacc ggcagtaata ttgccgtgag tatccccgta agccctggta cggattgcat
    37921 tgacgagatc aagagccgtt ccggcatcac caccgctgcc tccgcgcaat acggcttcgg
    37981 catacatcag gtaaacatca gccagcctga ataacgggaa atcggtatcc acataagtat
    38041 tatccgatcc tgcggcaccc gtgctggtga cattcttcca ttttgtgatg gcgtaaccgt
    38101 tggtaaagtc cgaaatatcc ttgattacca aactttgtcc atcggtccag aacatggcgc
    38161 gtttgtcgat gctctgacgg gtcaccgtat aggtgtaatc cggggcgcca agtttcatgg
    38221 tgataacata ggaaccgggt tcagcaaccg ggatattgtc cccgccggct tcgagcgtac
    38281 cattattgcc gttatccccg taattgatat cccagccgtc attggcccgg aattttatct
    38341 caccctgaac aaggtcgaca gtggcagtcc agatgccagc ctggttgtta ttttcgtcgg
    38401 gaacgtaggt catgttctga tccgagcccc aacctccgga ggtagccgat cctataatgc
    38461 cccaatcggt ctttataatg gtatatgtca acgcgttaag gtcaacattt actttatagt
    38521 aaccggcatc ggcaacctga atattgtcac cccccggttc aagcgtaccg tcagcaccgg
    38581 tgtcgcccca gttggtatcc caggatccca gggcaaattt gaattgtgtg ccggcatctt
    38641 taaaatagag gtaaccttca tatttattgt cgctgtttac cgaagccagg acagtattgg
    38701 gatcagcagg gttccagccc tggtagcttc cggggcagtt taataccggg taagcatttt
    38761 ttattccctt acgcaaagac ttcgggaaaa cggaaccttc aaatttatca accagggcgc
    38821 tggtagtccg gataccgccc caaccgcctc cgattccgaa atcggcaggg accatgcttc
    38881 cgccaacagc agcatgaacg atgaatgtgg ttccgcccca ggtccgcgtt ctcaagccgt
    38941 cgaatacgat agggaagata atttcattgc ttttctggtt atcagccttg aaaaggtttt
    39001 catattccgg ttcaagggaa tatcctgcat tgataacctt ggaagcatag gtgatacatt
    39061 cggtatattt aggagttccg atgtacactt cggcattcag gtacaattta gccaggagcg
    39121 tccaggcggc agctttatcg gcacggccgt attcattggc gccgggatct ttcaactgcg
    39181 attgaatgtc gagcagttcg ctttcaacaa agccatacag atcggcacgg gttttctgtt
    39241 ccgggaagaa tttccctaca gggtcgtttt cggtgacgaa aggagtattt gcaaaaagat
    39301 caaccgcatg atagtaactc agcgccctaa ggaaccttgc ttcggcgcgg tataaggcca
    39361 catcgctgcg caggcttccg gtaacgccgc gttcatcgag tttggcgtcg gtggtctggc
    39421 ggagaaattc attgcaaaat gcaatctggg caaaaatgcg ggagtacatt gccgctacaa
    39481 acacgtcgct tgatccccat ttctgaaaat ggaaatcctt gatggtctgg tcgttccagc
    39541 caatcacggc ttcatcggtg gtaagttcct ggtggtacca gaaagcgcgc aggtattcac
    39601 cgaaaccttc gtcaatgcct tcgatgtcag ggtttccggc tgctcctacc tgcccggtaa
    39661 ctgccaggcc tgaatacaac ttggccagaa ccatcttata ggaagcagga tccttgtaaa
    39721 catcttcggc agaaataaca tcggggtcaa tgggcagtgt atccagatct tttatacagg
    39781 ataccataag caaggcgatc ccggccatga agagtgttat atggagtatt cttttcattt
    39841 tctcagaggt tttaaatgtt agaataatac gtttacaccg agcgcaaata ttctgggcct
    39901 gggataaatc tgtttatcaa gtccgtcgaa aatttcagga tcaataccct tgtatttcgt
    39961 aatggtgaat acattgttca cggtaaacga aagcgtcata ttggctgtat tcttatacag
    40021 gttgccaacc atgtaagtaa gggtgatgta atccatctta aagaaggatg catttttgat
    40081 aaagtaatcc gacaggtact gcggtgatgt aaagtccaca tcgaacaccg aggaggtgat
    40141 attcgacagg tacggccctt cggaacggta caggcgctcg taaacgccgt tttcggagtt
    40201 tacattgtcg taaacataat ttccgatatt ggcatgtccg gaaaacgaca aatcaaactt
    40261 tttataagtc agcttcgagg aaataccgat tacataatca gcattagggc tcttatagcg
    40321 gtaccggtca ttatcgttga taataccgtc gttgttcaga tcggcgtaca ggccttgaat
    40381 aggctttccg tcctggtcgt aaacctgttt ataaacatag tatgagaaca tgggatatcc
    40441 gacactatgc acctggactg tattccccac gccgccttgt atatttccga ccagaactcc
    40501 ggtatagttc ggatcgtcaa cggtcgtaag tttggtgatc tcattcttgt tgatggttag
    40561 gttggcgcca atttcccagt tcagtttttc actggtcacc ggacggccta cgaggctgaa
    40621 ttcccagcct ttgttttcca tatcgccgat attggtggtc acatagttcg acagattgga
    40681 gccagccggg aaggttatcc ggttaataag gtctgtagta taacgctcat agtattcaac
    40741 cgatcccgtg atccggtcgt tgaagaaacc aaagtcgaga ccgatgttcc gcgtctccgt
    40801 ttcttcccat ttgatgttgg catcatactc ttcgggacgc agcgtgtaat agaaagtgtt
    40861 ccccaacaga taactggtcc agacgtcgct ggtggtatac accggaatat aaggataggc
    40921 attgtcggaa atatcctgct gacctgtaag gccccatcca acccggagtt tcagttgaga
    40981 aacttgtttt acatctttga ggaaggattc ttcattaatc ttccatgcca gcgctgccga
    41041 agggaataag ccccagcggt tgtccttcga aaaccttgac gacccgtcat cacggcaggt
    41101 gaaggtaagc agatacctgt ccagaagggt atagttgaaa cgtccgaaga aagaaacgag
    41161 gaaatattcg ttggcatcaa tttccgtttt gggtgcggtg tgatcaacat tattatgata
    41221 ggtgttgatc caggaataga aatgttgcca ggaataaccg cccatcacat cgaatttgct
    41281 gtagatcgat ttcacatctt taacatagtt gaggtagaaa tcgaatacag aattttcttt
    41341 agtctggtca taaacacggt caataccgcc gtcgttataa ttcagggttg catatttggg
    41401 cacataaaca gttccatcgc tgcctgaata gtcgtaacct atgttcatat ttgcccttag
    41461 atcaggcaga aaatggaatt tgtaatcaaa ctgcatattc ccgacaaaac ggagggcatc
    41521 ggcctgatcg ttttttaaca taagggtagc cacaggatta tcggtagcct ggtcaacagg
    41581 ttctccattg gtttgcagcc aggcccagta tccgccgttt tcattaacag cagtaaccgg
    41641 gtgggtagga tcgaactgga tggcctggtt aatggcggca ttatcggcaa acctgttgtg
    41701 gacaatcatc catttaccat tcatgttaac tttcaggtga tcgtcgagga gactgggatt
    41761 caggctaatt cccatcgtgg tccttttcat attatcggtt ttcaggatac catcctgcca
    41821 catataaccg gccgaaacgc ggaaaggcat ttcatgaaac tgaccggtaa cgctaacatc
    41881 atggtcggta ctgaaggcat tctgataaat ttccttttgc cagtcagttt tagcatcacc
    41941 cagcaaggaa gtgaaatttg gatacttttc gttttccagt ttgatgaaat catctgcact
    42001 gagaacgtca atcactttgg gaatagtgga atacgtcagg ttgcctgagt attcaacatg
    42061 caatttatct cctgctttcc cttttttcgt tgtaatcagg attacgccat tcgaagcgcg
    42121 ggagccgtaa atagccgttg ccgaggcatc tttgagtacg gtaaaagttt caatttcgtt
    42181 aggattgacc atgctcaacg ggctacgaac gcctgagata gtgctggttc cgtcaaagtt
    42241 tttacctttt tccacaggga cgccgtcgat aatgacgagg ggttcattgc tggcccgcaa
    42301 cgaagaacct cccctgatac gaatggtcgc gtcgccacca ggggcaccgc ctgcagtggt
    42361 aaccgtgacg ccagctactt taccgacgag taattgctgc ggcgaagaaa tagccccttt
    42421 gttgaaatct ttgtcaccga gggcagtaag ggcgccggta gcatcgccct tcctcacctg
    42481 gccgtaacca atgaccacga cctcgctgag gccggtgaca gccggttgta gtgcaatgtt
    42541 aaaaatgcgt tgtgagccaa ctgctacttc ctgtgtagtg aaccctatga acgagagcac
    42601 cagggtagca tccggtctta ccgtgagggc aaatctgccg tccaggtcag taacggttcc
    42661 tgtagttgtc cctttaacaa gaatggatac tcccggcaag gtcgaattgt cggatgcatc
    42721 ggtcaccttt cctgatattt tcacttcctg ggccattgct atcgttgtga cagcccagga
    42781 aaggagaagc aacagtaccc gcaagagttg agattgtttc ttcatagaac tggttttgag
    42841 tagttaaaca ttggttaaaa aatgattagt aatgttgatt atggtgtttt agatgatgat
    42901 ttatgaaaca aattaatttt ctccctttat ggccagtttt catgtacatg aaagaagaat
    42961 tattccgggc gattattcct gactcaaatc taaagaaact tttcatattt ggttcatcct
    43021 atatgctgat ttagaggtta tttcgtcatt ttttttacga aaccgtttcc gcaaacgttt
    43081 gcaataatct ttttaaggcc tttctttaat ttataaagcc ataatttcaa tcattgctga
    43141 atgcttacga ttcaaaactg ttccttttta aatggttaat ttcatcatgt gggctgatca
    43201 atatttcgat tcttcctata aagttatatc cattaatgag gcaaaatcaa ctatttttta
    43261 aaatatatcg atcaacatca gtgttgagat ttccttataa cccgttgttg cctttaaaag
    43321 gaagagaaac aacgaaaaat ggctttcctt gaataatatg acgaagattc ccatttcggt
    43381 tgtaccggat cgaatccggt tataatcata gctgaaatca ttatctttga taataataac
    43441 gtgaccatcg aaaatccggc aatccgattt taaaatatgg ctactattcc gaaattgttc
    43501 gtccccaaag gcaatcggaa ttccccgttg cggctgatcc tgatattacc tttgctcttc
    43561 ctgatggccg gatgcgagaa aaaggacacg tccgcccccc ggaataacag gcaacctgta
    43621 tattatacca tcggtcagtt ttgcatggga tgcgacctgt cggtggttaa ccaggttgaa
    43681 gatcacggag gcgttttcgc agattcgtca gtggcaggtg atccttacag catattacac
    43741 agccatggcg ccaaccttgt ccggctgcgt ctctggcata atcctcaatg gacaaaggaa
    43801 gtgtatgggg cagaagggca tcaactttac aacgacctgg ccgatgttga aaaaagtatg
    43861 tttcgggcaa aacagaacgg gatgaaagtt tgcctcgatt tccattattc cgatacctgg
    43921 gctgatgcag gcgcccagga ggttccggcg gcatgggccg gtatcacgag cctggatgta
    43981 ctgaaagatt cagtatataa ctatactttc agagtactgt catatctgaa aaatgaaagt
    44041 ctaatgcctt acatggttca gatcggtaac gagatcaatt gcggcatgtg ttattccggt
    44101 gccgatgcag actttccaaa tctgaatgcc tgtcagggac actgggccga actgggcggg
    44161 gttattaaca gcgctataaa tgccgttcgc gatgtttccg tagggaacac aaatgaagtt
    44221 ctggtagcgc tccatatcgc tgatccttca aacgtggaat ggtggctgga taacattacc
    44281 acaacaggcg gagttagcga ttatgatatc atcggctttt cctattaccc tttgtggcat
    44341 accggtataa aactctcaaa tatcagttac gtaaccggac gcgtcaggga aaaatacggc
    44401 aagcaggtta tggttttcga gacggcatat ccctggacgg cagacggaaa cgataattat
    44461 accaacctgc ttggcggcca gacgcccgtt tcgggatacc ctttcacgca acagggccag
    44521 cttgatttca tgaaaacctt aacccaggaa gtgattgatg gcggaggtat cggcgtcatt
    44581 tactgggaac ctgcctggat tacctcgcag atgaaagacc tgtggggatc gggctcatca
    44641 tgggaaaatg ccacattttt tgatttcagc ggcaatacct tgccggtaat cgactatatg
    44701 aattaccctt acacgttccc cggcaaataa aaacacctcc cccagttttc acggaagcac
    44761 tcacaccaga aattttcaaa atatgcccaa atctgctccc cttaccatta aagatatagc
    44821 acggaacctc ggcgtttcgg tatccacggt ttccagggcg ctgaaggatc atcctgacat
    44881 cagccccgag actaaactca tgatacaggc ttatgctgaa cagggtaatt atcgccccaa
    44941 tgtgctggct ctaagtcttc gacggcagaa gtcatatacc atcgggctca tcataccggc
    45001 tattgtgcat cacttttttt catcggtgat cagcggcatg gaggaactgg cttacaaaga
    45061 aggatataac cttattatct gccagagtaa cgaaaaccag catcgtgaag ccattaacct
    45121 gcaaacgctt atcgatcaca gggttgatgg gatactggta tcgctgagca aaaccacgca
    45181 gtctttcgat ccctttatcg aagcttttgc atcagggttg cccattgtct ttttcgaccg
    45241 tgtttgtccc gaagttgaaa cggaccgtat cctcaccgat gatttccatg gagggcgtat
    45301 tgcaacggag catctgatta aaaggggctg taaaaacatc ctgcatcttg cggctcccca
    45361 gcatcttctc atcgggaaag gccggtttga cggatattgc gaagcgctta aaagcaaagg
    45421 ccttgcattc aggaatgagt ttactttgaa atgtgatacc ccggaagaag taatggccct
    45481 gaaaaaccac cttattgacc ttgcgcccca tattgacgga attttcgctg tgaacgacag
    45541 caccgccatt gccgtaatcc aggtgctgca gaaggccggg ttcagcgttc cggacgacat
    45601 agctgttgta ggttttggcg atgggcccaa tgccactatc attcatcctg aactaaccac
    45661 agtggtgcaa aaaggatacg aaatgggaag ggcggctgta agactgttgc tccagcgcct
    45721 caacaaccct tcagcagcca tcagcccgca aacaatcagt tttaaaccag tgatgaaaat
    45781 cagggagtcg gcttaaaaaa tgaaatcatg gttgatttcg ggcccatcta aacccgatcc
    45841 ggcaattttc ttcccggcct cagaaggttt cttcgagaag ttgcatcgcc acttccttat
    45901 ccagcgacaa ctgatgctgc aattgctcca gccctccgaa tttcctttca tcacgtattc
    45961 tttcaataaa cgacagcgaa attttttcga agtaaatttc agtatcaaaa tcaaaaatat
    46021 ttacctcgat agtcaggtga ttcgcattaa tggttggccg cgtcccgatg ttgctcattc
    46081 ccttgaaccg tttcccgttc cattccacca gcgaagcata aacgccgttt gccgggatta
    46141 ttttataggg cgaatcgggt tcaatattgg cggtagggaa gtggatcagt ttgccgatct
    46201 ggttgccccg gtataccttg ccgctgaacg aatacggata tcccagcatg caaccggcca
    46261 gtttcacgtt gccggcggca agcgccttcc tgatggaggt tgaactgact tcctcaccgc
    46321 aataacttac cgccttcacc tgctctattt caactcccga ggctgcccct atctcaacca
    46381 gcagggcata atcgccttca cggttacggc caaacatgtg gtcgtgaccg gtgataatct
    46441 tttgcacatg cagcgaatcg ataaggacaa acctgataaa ttgttgcgga tcggttccgg
    46501 caaaagattt atcaaatgga atgattactg cagcatccac tccggcgagt tcaaaccttt
    46561 caagtttttc gtccagcgtc tggagcagtt ttacctgctg ataggtatct agaacaaggc
    46621 gcgggtgggg ttcaaaagtc acaataaccg attcggtctg cagacgtttt gcagatgcca
    46681 caaggtgatt gataatttcc atatgccccc ggtgaacccc gtcgaatacg ccaaccgttg
    46741 ccacaggctt tgtggctgaa gcaaattcgg atatggaatg ataaatacgc actgggactg
    46801 tttacgatgg aggcgctggc tcgtcttagg attcggcgtg aatgactgac tttagttata
    46861 caagttcatg ctctgccagg tatgcagcaa tctgaaccgc gttggtagca gctcccttcc
    46921 gcaggttatc ggatacaatc cagagattca gagattttgg ctgtgactcg tcacgacgga
    46981 tcctcccgac aaatacctca tcgcgacctt cggcatataa aggcatcgga tatattgact
    47041 tctccggatc atcctgtacc acgatcccct ttgttttcga caacaggctg aacactgcct
    47101 tgagatcaaa atcattgacg aattcaatat taacggcttc ggaatggccg ccggtcaccg
    47161 gcactcttac aacggttgcc gtaacattga tcgaattgtc gccaagaatt ttacgggttt
    47221 cgttcaggag tttaatctct tcggaagtat acccgttgct ttcgaatgtt ccgccatgag
    47281 ggaacaggtt catatcgata ggatgcgggt aaacgcgctc gccgctgata cccgcccgtt
    47341 cattattcag ttgtacgact gctttttgcc ctgttccggt aaccgattga taggtggaaa
    47401 ccacaacacg tttaatggta aatgccttgt gtaaaggagc cagggtaagc gccatctgta
    47461 tagttgaaca attcgggttt gcaataatct tatgtgaagg ggtaagtatc tcggcgttaa
    47521 tctcaggtac aatcagcgga atatcggcat tcattctcca ggccgaacta ttgtcgataa
    47581 cggtagtacc ggcagcggca aactggggtg caaattcgcg cgatacggca gccccggctg
    47641 aaaagatggc tatttcagga tgctgttcaa tggcttcggt aatactaacg acagggtaag
    47701 gcatgctgtt gaaatgcaaa acctttccca gtgagcgctc cgatgcgaca ggtattactt
    47761 ccgaaatttt atcgaaagac agataacgtg cttcttccag cactttcatc ataacgctgc
    47821 caaccaaccc ggtagctccg acaatggcta ttttcatctt ctaaaattat gttttcaatc
    47881 ggactgcaaa aatactatat ttacattcgg gcagcgaaaa gcatttttta tatgaaaaaa
    47941 aaacatttta cgattattct gttattactt tcctgcaggg ttcaggctca ggtaattgac
    48001 gatttctcag atggcgattt tacgtccagc ccttcatgga cgggagatat agccagtttc
    48061 aaggtaaaca catcctttga attgcaattg aattcttcgg gcgacaacat ttcggcgctt
    48121 gccacggcct gcccaatggc ggactcgatg gagtggaact ttaaagtcag gcttgccttc
    48181 agcccttccg acaataataa tgcgcggata tacctggtat cggaccagca ggacctgaaa
    48241 tcgaacctga acggatacta cctgaaactg ggggaatccg gttcggacga tgccattgaa
    48301 ctgatcaaac aatcagggaa tatccatact tctctttgtc gcggcacccc gggcctgctg
    48361 gccactgcat ttaccttacg tattcgcgta atccgcgaca attcaggcaa ctggagcatc
    48421 tttgccgatc cttcgggagg aaatgcttat cacccggagg caacagctac cgatgatacc
    48481 tatacttcgg catcgtggtt gggcatctat tgcaaataca ccagcagcaa cagcacaaaa
    48541 ttttattttg atgattttta cgccggaacc gttattattg ataaaacccc gcccgggttg
    48601 ttatcggcca gcttgcttac ctctaacgcc cttgacctgc ggttttcgga gagcatcgac
    48661 cttattactg gccaggatgt tgccaattat acgctggacc atggtatcgg ccaccccgcc
    48721 attgtgttac gcgacgccca ggatcttaca ctggtgcatc tgctgtttag tccggagctt
    48781 acaagcgaca ttacttatca ccttaccatt gaagggatta aagacctttc cgggaatgag
    48841 attgttgcgc tggacgtacc tgtgagttgg caccaggtta aattgtttga cgttctgatc
    48901 aatgaaatca tggccgatcc tgatcccccg acaggactgc cggaatcgga atatattgaa
    48961 ctatacaacc ggagcacttt cccggtaaac ctggaaggat ggatgatccg ggtcggaagc
    49021 agcgaaaagg attttccggc ttatacgctt cctgccggtg gttttgtgat actggcagct
    49081 aactcgtcgg agccatcgct cacaggatac gggcctttca tcggattctc cggctttacc
    49141 attgtaaata cgggaactga aataacattg ttcgacaaag accggcacgt cattcattcc
    49201 atatccttta ccgaagactg gtaccggaac gattataaaa aagaaggagg ctggtcgctg
    49261 gagcagattg acccgttgaa tccctgcggc gaagcggcta actggattgc ctcaaacgat
    49321 gcttcgggcg gcactcccgg aaagatgaac aatgccaacg cttctaattc tgacaatacc
    49381 cgtcctgtca tccaaaggat cggagttgat gacccttggc atatccccgt gtggttttcg
    49441 gaacccatgg acagcaccac cctgctggac ccgtcgaatt gcattattga ccagggaatc
    49501 ggcaacccgg catccgtaac gctttcaggc cctgattaca gaaaaatcac catgctgctt
    49561 gccaatccta ttgtacaagg aaccatttat acgctgacca ttgaaggttc gtttaccgat
    49621 tgcgccggaa acatgatttc aggaacagct atggcaaagt tcgccattcc ttccgcaaca
    49681 aaagccaatg aattaataat caatgaggtg ctttttaatc ctgcaccatc ctgtactgat
    49741 tttgtagaga tttacaaccg ctcagccaat gtcttcgatt tacaatggtt gacgctggcc
    49801 gggtatgata ctgtcacgct cggattatcg gacatttccc gcatctgtca ggaatcaagg
    49861 ctgatttttc cgggcgaata ttatgcagtt accacggata cagcggcagt aaaagcttgt
    49921 tacttaacta cgaatcccgg cggctttatc caggttagcg gtctgccttc gatgaacgat
    49981 gatgaaggtg ccatcgcact ggccctggga acaggcgaaa taatcgaccg aatgatttac
    50041 agcgacaaga tgcattaccc gctgctgacc ggcaccgaag gcgtttcgct tgaacgcatc
    50101 aatcctgaac agccgtcaag cattttatcc aattggcatt cggcatctga aacagtcggg
    50161 tatgcgaccc ctgcttatca gaattcacaa tacgggcaag tcgcccagtc cgggaatgaa
    50221 atctctcttt caccaagtgt attttcacct gataatgatg gcaaagatga ttatctgacc
    50281 atctcttata catttgaaca atccggcaac aatgcccgcg ttagtgtgta tgatgcaacc
    50341 ggacggctgg tacgaagact ggtaaacaat gaactctgcg ggactaacgg agcgtttacc
    50401 tgggatggct tcaccgatga ccgcacccta gccctggccg gaagatatat catctatatt
    50461 gatattttcg attccgaagg gaaggtaaaa cacttcaaga agacgaccgt cctgggctgc
    50521 aagttataat tcctattcat gagcaaaggc gattgacgca acacttacct taacgccggg
    50581 caaagccagc aaaacgttgg cacatgattc aatagtggct ccggttgtta ccacatcatc
    50641 aaccagtaat agatggcgac cttccagtct ggaagaatcg gttacggcaa atatttcttt
    50701 tacattttcc catcgtgcca ggcgggattt ttttgtctgc gtttcggtag ccactttccg
    50761 gatgaacgac ccggcttcca tcctgattcc catagcttcg gataatccga tggcaaactg
    50821 ttcactctga ttgtaaccgc gtttccgctg tttccgcgga tgaaggggaa ctggcagtac
    50881 agcgctgata tcatgatagg caggcaattc tttcagttca tttccgagta gttttccaag
    50941 gtaaatgcca acctcttttt tgcctttgta tttcaactgg tgtatcaggt gctgtacgtt
    51001 tccgcccttg ctgaaatagt aaagcgcggt agcctgttca acccttaccc ttccccagaa
    51061 aaccctgtcg agcgggttgc cgggatgcag gtgataattt gttttcggaa ggtgctgaag
    51121 acaattggtg cacagcactt tttcgtgcct gaacagtgaa ttcccgcagg cataacaggt
    51181 gtcgggataa aagaaggaaa tgaaatccct gaaaacagat aatactttca tcattgcaat
    51241 ttcaataaaa gatcagctcc atcttcctgg tattttctgg ccgcaaaact tgcaggctcc
    51301 gttcacgata taattagtga caaccataaa acccttcctt ataaccagta attttttaca
    51361 ttttgggcac ctggtattct ccatgtcagt gcccggaaga tttcccacat aaacgtaatt
    51421 cattccttcc gagagagcta tttcgcgggc tttctgcaga atcgaaaccg gtgttggagg
    51481 caactgagtc aatttataca ggggtgtgaa ccggctgaaa tgcaacggac agtcatcaag
    51541 gccgtttttc accagccagt tgcacatact cctgataata tcaggattat cagtccaggt
    51601 cgggacaata aggtttgtga tctcaagcca gacgccttcc tcctgaagga tcttcaaggt
    51661 acgcagcacc ggcgcaagtt tccccgaatt tagtttctga taagtgtttt ccgaaaatcc
    51721 tttcaggtca atattggctg catcgaggta tttacaaagc atccgaagtg gcttctcatt
    51781 gatgtagcca ttcgatttat aaacattttt taacccttgc ctgcgggcaa tcacggcagt
    51841 atcatacaca tattcataaa acaccgtagg ctctgcatac gtataggcaa taatccggca
    51901 cttttttttc aggcattcgt caaccacttt tgcaggcata aggtcataat tggaggtctc
    51961 tttggggctt ttctgcgaga tttcccaatt ctggcaattg agacaggaaa aattgcatcc
    52021 ggctgttgca atagagaatg cctgtgaggt aggaaaaaaa tgaaaaaacg gctttttttc
    52081 gataggatct atatgaacgg cgcacggatt tccgtatgca atgctgtaca acttatcgcc
    52141 tatgttgata cgattgcggc atttgcttgt ttccttgcgt ttcagtgtac attcgttggg
    52201 acaattggta cactgaacac ccctgtcggt aatcctgtaa tggtatgctt cacggctcca
    52261 tttccacaat ccgtttgctg ccgccggcaa ttctctatct aagcttaggg ccgacacctg
    52321 ccggtggttc agtccgcatg ctgcacagcc taaaacagcc agacccgatt ttaagaatgc
    52381 ccgtttatcc atctatgaaa ttatcagata caaatttaca tcatgccgga tcgtaatctg
    52441 tgcctgcaaa aatttttcag gacagggctt cgaacaaatt atcgttattt tccacaaact
    52501 aacgaaagct ctgaaacttg ctaccaacga tccaattatc ctgctctctt cctttttaat
    52561 gccattatta atacagaaag caggcaagca cccgagattg ccatcagcgt cccggacaac
    52621 cccagcatag gcatcagcca ggtgctcacg attaatgccc caatggctga gccaagcaga
    52681 tcggcgctgt aaagcgagcc ggcaactgac cggacttcgc cctgtttgac aacagcggca
    52741 acctggaaaa tcattcccgt caggatggat agcatcaacg ttaatatcgc gaaacctgca
    52801 tgtatggcca ctgtagtaga atgaaaccga tggaatccga ttatggcaac aaggattacc
    52861 agtatacaaa tggccatggc aaattgcagt ctgacaagaa atatgctgga tacaattctg
    52921 aaatactttc gtgcgtaagc agcgcccagc gccagtccca gcatgaaaac cgtaatgatc
    52981 atgcccgcag ccaggaatat atatccataa ataacctgga aagcaagcaa tatgatgact
    53041 tcggaagccg atgccgcgaa acctgctgta aacagcccga ctgtagtact atcggaccgg
    53101 aatagcaccg ggagtaataa caataggagc aacaggggca ggtaacccag gtcatgtccg
    53161 aaataatcca gacagtattt tacctggctt tgataggcaa gcggtttaaa atcgcggttt
    53221 atcggagctc ctggctgcaa atcctgcata atagtccggc tgcgctgctg taacgactca
    53281 tcctcaagat aatacgggtt aacgtaatct gtttcaacgc ctctttcagt ctggagggag
    53341 gccatatcaa tccttagagc gctgtcagaa cccaggaaat aatccctgtc gcccggaacc
    53401 accagtacat ttttaaacac ctttttcagg gtggcataca tgatcagatg cgcccgtttc
    53461 gaactatagc ccatataatc tgccgttgcc atcaggctga gcgaaacgac tccgcctttc
    53521 cgtaacagcg gttttagaat ggtcatgaat tcaaccgaat aaaaacggtt tagctgcaag
    53581 gtcgacggat ccggtagatt gagcaggata acatcatatc tggaacttcc tgctccgtta
    53641 ttatgggtag cctctctttt aacaagccgg tcagtgaacg agcgcggatc gcttagtact
    53701 actttaagcc tgtcatcttc cggcatttcc gataatgtgc cggctacctg tgccagccag
    53761 ggattcattt caatgttatc gagatctttg attggatatt tcagaatttc gcctactatg
    53821 cctgaaaaac cgccaccgat cagcagcaca ttcaccggat tttttcgctg aagcatcgcg
    53881 taatgaaccg tttcctcatt ggtgattaca ttattcgttg tatacagcaa cgtgttattt
    53941 tcatatacat ttttctgtcc cccgctttcc gttacgacca tattcccaaa aggagtatcc
    54001 ctgtaagtca ggattttctg cccggggaaa agaaatttcc gggtaacagt atccggattt
    54061 acgctgagta ccagggcaac aaagactatt gaaataacag ctatacccgg caacagtgat
    54121 ttttgtcttt ctttaaacag gaaccagccg aggatcaagg cattcacaat aacaataacg
    54181 gtaagggcct ggaatggttt caggaaaaag accagaatga agttaaaaag agcccctccg
    54241 gtcacactgc cgatgttctc ccaggaatag accaacccag tggcagggtc cttcgaatga
    54301 aaagtcataa ttgatgacaa tcttgtaaaa agggaacctg acagaagaca aaacggcgcc
    54361 aggagaaata ccgacataag aaaaacgttg tatattccgg ccatactgcc tgtcgtaaaa
    54421 acgacatttc gcatagaatc aagaataaac agagtaaccg gcgccaggat tgacaaagcc
    54481 aggagagtga aagcgattct gctggtctgt cttttctcat ggtgaacgaa ttttccaagc
    54541 cacgctccca gcccggtaag aagcatccag ttggcaagga ttactccgat ttcaagttcg
    54601 ttgccgttaa aaacagccag aaactcacgc aacagaatga tctgcgatgc catggctgtc
    54661 agcccgaggc tgaatggaag caacagaaaa attgtttttt cccctgattg gcgcttatca
    54721 aaaggatcct tttgatcatc cgtaatttgg gctattcctt tttgtttgga tttctgaacc
    54781 cgtgagcggg gttttataat ttcaagaaaa tatttcaaat gccaagaaag atgcagcgag
    54841 gcaatgacca ataacccgat accgaagtct acatgacaga tcaggaaagt agctgctgcc
    54901 ggcaggtcga atttaagatt aacctgcagg gccagaagga aaccgaggcc ggcagctccc
    54961 aggaaggcgg ccaataacag aacattccag tatttccggt gtgtaatgcg cgatattatg
    55021 cccaggtaaa ccatcagcaa cgatgcgaaa taaagaacaa ggcacagaag cggaatgctc
    55081 agaatggcat aaggtttatc aatcatgacc ctaaattagg aaaaaagcac tccggttcat
    55141 aataaaaagc cactgttact tttatctgcc gaagagcaaa tacgaggtag caggatggta
    55201 cagggcaaaa ttgaaacaga agatgagttc agcccgaaaa tctggccggc aagtgaaata
    55261 ggatattgat aaatcagccc cgaaggggct gaactaccgc cagcccgaac atctcgccgg
    55321 aatccacaag ttcagttgtt gaaaaaccag ccccgaaggg gctaaatttc aataagggca
    55381 ggcgataacc ctctctgaag tatgcccctc acagcagaca cagccccgaa ggggctaaat
    55441 ttcaataaga gcgggtgata accatcactg aagtaagccc ctcacatcag gcacagcccc
    55501 gaaggggcta aatttcaatt agagcggtca atcaccagca ctgatgtatg cccctcacag
    55561 caggcacagc cccgaagggg ctaaatttca attagagcgg tcaatcacca gcactgatgt
    55621 atgcccctca cagcaggcac agccccgaag gggctaaatt tcaattagag cggtcaatca
    55681 ccagcactga tgtatgcccc tcacagcagg cacagccccg aaggggctaa atttcaataa
    55741 gggcaggcga taaccctctc tgaagtaagc ccctcacagc agacatagcc ccgaaggggc
    55801 taaatttcaa taagagcggg cgataacctg cactgatgta tgcccctcac agcaggcaca
    55861 gccccgaagg ggctaaattt caataagagc gggtgataac catcactgaa gtaagcccct
    55921 cacatcaggc acagccccga aggggctgaa cagactaaag aaaaaagtac ttttcatcaa
    55981 tggtaatccc ctgctctatc agcaactgcc gaaact
//
LOCUS       DAOPYV010000009        55847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_433_length_55847_cov_10.831015, whole genome shotgun sequence.
ACCESSION   DAOPYV010000009 DAOPYV010000000
VERSION     DAOPYV010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 55847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55847
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_433_length_55847_cov_10.831015"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..833
                     /locus_tag="PKI35_01980"
     CDS             168..833
                     /locus_tag="PKI35_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HNX43176.1"
                     /translation="MDNTADNFVSGLHGWHSKALGEKTAESLRKNNFRAEYLELPEEA
                     RNRILSQINPGDRIAFGGSYTVKQLGLPELIEKIPVVILDHNAPGLDAAQKLDIMRQQ
                     QVCDVFICSANAITLDGHIFNVDGTCNRISAMVFGPKKVIVVAGVNKICQDEAAAWER
                     IRMIAAPVNMKRLNRRTPCTVTGYCTDCDSPERGCNAYLVLKKKPALADFSVFIVNKS
                     LGF"
     gene            921..2282
                     /gene="purB"
                     /locus_tag="PKI35_01985"
     CDS             921..2282
                     /gene="purB"
                     /locus_tag="PKI35_01985"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNX43177.1"
                     /translation="MELSPINAISPVDGRYRKQVENLSYYFSEAALINYRVFVEVEYF
                     VALCNLPLPQLAEVDKKMISQIKSVYRNFTFNDAQEVKEIEAVTNHDVKAVEYFLKKQ
                     LDKAGLGKYREFVHFGLTSQDINNTAMPYALKQAMNEVVLPIVESLVEKLLKKSKEWQ
                     KVSMLARTHGQPASPTLLGKEMYVFVERLKKQLKLLKAVPFEAKFGGATGNMNAHYIA
                     YPDVDWIAFADTFVKKQLGLERQHTTTQIEHYDSMAALFDALKRINTILINFSRDIWS
                     YISMEYFRQKITAGEVGSSTMPHKVNPIDFENAEGNLAIANAIFEFLSSKLPVSRLQR
                     DLTDSTVSRNIGVPLAHMVIALKSLLKGIDKLILNEAKLQSDLEDNWAVVSEAIQTIL
                     RREGFPKPYEALKELTRTNKHIDKKAISDFIDGLNLPAAVKKELHKVTPSNYTGKFEI
                     REK"
     gene            2349..2807
                     /locus_tag="PKI35_01990"
     CDS             2349..2807
                     /locus_tag="PKI35_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HNX43178.1"
                     /translation="MDLKDYLAISGKPGLYKTVAQSKTGVIVESLIDGKRLQAFASDK
                     ISSLGEISIYTDGDDMPVREVFRLMYEKKDEVKLPDLKGGDKDIRTYFETVMPQFDRD
                     RFYISHMRKVISWFILLISKDITDFSAPAEETSPGEEKEDTPPAEPKDTE"
     gene            2874..3647
                     /locus_tag="PKI35_01995"
     CDS             2874..3647
                     /locus_tag="PKI35_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNX43179.1"
                     /translation="MTKYIHDFRIIQNRQVNPGCHVLELLCPVKLPEIQPGQFAQLLV
                     EKSPATYLRRPISIYDADPEKNTLSLMIKVVGEGTASLVKLGEGDSLNLVYPLGNSFS
                     VPEGKKVLLVGGGVGIAPMLLLAKRLKAEGKEITILIGGRSFQDILEAERYEAYGSVG
                     ITTDDGSLGFKGLVTEHPAFAAAGEYAMIYACGPDPMMKAVARIAHKMGAPCEVSLEN
                     LMACGIGACLCCVAETTTGNRTTCVDGPVFNSGDLVGWE"
     gene            3883..4794
                     /locus_tag="PKI35_02000"
     CDS             3883..4794
                     /locus_tag="PKI35_02000"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNX43180.1"
                     /translation="MADLSVDINKLHLKNPVMPASGTYGYGPEFDDFYKVSRLGAIIT
                     KACTSKHREGNRYPRMAETPMGMLNSVGLQNKGVDYFISSIYPEISHYDTNIIVNVSD
                     STIDGYVEVAEKLNRLEKVSAIEVNISCPNVKEGGMAFGTSCQMAGLVTKAVRKAYDK
                     TLIVKLSPNVTSIADIALAVEAEGADSVSLINTLLGMAIDAENRRPLLSTVTGGLSGP
                     AVKPVALRMVWQVARAVKVPVIGMGGISNASDAIEFLLAGATAIEIGTANFVNPMVCN
                     QVIDGIEAYLDRHHCKSVKEIIGALRV"
     gene            4826..6115
                     /gene="purD"
                     /locus_tag="PKI35_02005"
     CDS             4826..6115
                     /gene="purD"
                     /locus_tag="PKI35_02005"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004354518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNX43181.1"
                     /translation="MQKKNVLIIGSGGREHALSWKIAQSPLLNRLFIAPGNAGTGFTG
                     INIPVSGNDFEAVKRAVLENDIEIVVVGPEDPLVNGIADFFASDGLLKDILVIGPGKA
                     GAMLEGSKDFAKGFMRRHKIPTAAYETFTAETIDEGLAFLKKLSPPYVLKADGLAAGK
                     GVLICRSLGEASSELKSMLVDSKFGKASSKVVIEEFLDGIELSVFVLTDGESYKILPE
                     AKDYKRAGEGDTGPNTGGMGSVSPVPFASGDFMQKVEERIIRPTIQGLREENIPYKGF
                     IFFGLMNVKGDPFVIEYNVRMGDPETEAVMPRIKSDLLELMIAIDEDRLSGFNLETYP
                     ETAVAVMMVSGGYPGAYQKGLTIDGLRNEELPVIFHAGTTYDESTKDVVTSGGRVLAV
                     TSMGVTLKDALRKAYAGAEKIKFKDSCYRRDIGKDLL"
     gene            6137..7117
                     /locus_tag="PKI35_02010"
     CDS             6137..7117
                     /locus_tag="PKI35_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43182.1"
                     /translation="MLKRLSKSGFLLQFILFLALGTLFWFPAFMHPKPASMIESAGPL
                     YRMIAGWLGGDASFSVILAAVLLVAEAFALHALFSSHDLIPRDSFIDAIIFLICMSWN
                     PELLYLNPAITAGIFILFALFMMMRMYGRTEPFQEILSASLSIAVASLIFQPASWFFI
                     GLWIGLLTYRITSWREWVISLIGMALPLIYMVSIYYLQGTLNENWVFLIAPFQEISID
                     IAPFSSGEISFLAAALFMLFYASAATLNSVSDKLISIRRKTWIITDFSLAGLFSILVA
                     HSAMKIGQLLLMAPLAFFLTYAAISIKKSRFNNFIIVIFLLFVIVIHYIL"
     gene            7121..7708
                     /locus_tag="PKI35_02015"
     CDS             7121..7708
                     /locus_tag="PKI35_02015"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNX43183.1"
                     /translation="MLLRHYQDKLVEAGVDEAGRGCLAGPVYAAAVILPPGFVNERLN
                     DSKKLTARQRDELRTIIEKEALSWSVAAADQNEIDRINILNASFLAMHRAIEALDHKP
                     ELLLIDGNRFRPYPEISHQCFVKGDATYMSIAAASVLAKTHRDEFMASIAGDYPEYGW
                     EQNKGYPTAFHRDAIVRHGISPFHRKSFSFQQKLF"
     gene            8397..9419
                     /locus_tag="PKI35_02020"
     CDS             8397..9419
                     /locus_tag="PKI35_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNX43184.1"
                     /translation="MNLKKPLFITALLLVVCLNTIAQGKPAPPDYAAYINKYSALALR
                     EMQLYRIPASITLSQGIIESNCGKSPLALQANNHFGIKCQKDWTGETFLMDDDEEQEC
                     FRKYNNAEESFRDHSLFLTTRQRYAVLFSLDMTDYKGWAKGLSKCGYATNPNYPEILI
                     RIIEENKLYRFDDTLVVARSDRNEENDDRKPEKEEFKEYRGGQLTVFFHDGYKMPSPA
                     DFTLREVSKLGRKVYENNGVPFIFALKNDTWQSLASEFGIYTFQVYKQNDLKQTDAIT
                     EGQMLYLEPKKRKGAVPACIVGPYDSMYSIAQHFGVQLKFLYKYNAMLPGDEPQKGST
                     IYLRKK"
     regulatory      9488..9608
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            9720..12308
                     /locus_tag="PKI35_02025"
     CDS             9720..12308
                     /locus_tag="PKI35_02025"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX43185.1"
                     /translation="MRKIKLFLICLASLICTNNLLFGQEKGEITGTVLDQSTMRPVEG
                     VSVDIPEIMQGSITDSSGRFLFKADAGTYRIQISHLSYEGVNRKVVISGAKATDMGTI
                     FLNPVVVSLKEVKIISSYISDRNTPVSVTTLASAQIEKQTGNKDYPELLNLTPGIYAT
                     KQGGGSGDDRLTLRGFQQENVALLLNGVPVSSMENGMVYWSNWMGLTDATEAIQVQRG
                     LGASKVAMNSVGGTVNIITKSSSSEKGGFLRYSISDFGNQKTSLQLSTGKMKNGLSLT
                     FLGSRTTGPGYVDGTYVNGWAYFLSLSYDAIPRHKFVFTAMGSPEKHGQKNYGSSVAD
                     FEKYGNKYNSEWGIYNGKVLNLSENFYHKPQISLNHYWNINEKMLLASSAYVSFGYGG
                     GRYSEAYNYGTATYYFRKNNQVDFDAVYLNNINNSDSVQLADGSTVTGYSKNILTHYR
                     ANHYWAGVLSTLHYDLAPGVKLMAGIHGRTFRSRLYEEVNDLMGGECWIEQYAWSLAG
                     VAGRRQVKKIGDVINVDNYSLMQYGNIFVQADYTKGKFNLFLASTASGTQYQRKDPYN
                     YPADPYSRKVFKQGFDTKAGVNYSLNKNTDFYLNIGYYSREPLYKFVYINYSNAVAKN
                     LNNEKIRAAELGYSFTNGDTFLRINGYVTDWRDKSLLSKEYIQLADSTLNRTLVRGLN
                     ALHMGIELEAATSPFRNLKTGLTLSLGNWKWTNDVTASIYDDNQVLVDSLKVYAKGLY
                     VGDAPQTQLGLYLEYSFWNGFDLTVNWIYYDRLYANFDPANRVTPGDRSQPYRLPAYG
                     MTDICLGYNFSVRQIPSRLQITCQNVFNKVAMLRGDDGADHSLDTFTGFWSMGRTFGI
                     SMKVMF"
     gene            complement(12424..15570)
                     /locus_tag="PKI35_02030"
     CDS             complement(12424..15570)
                     /locus_tag="PKI35_02030"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX43186.1"
                     /translation="MKAFIFHAFFIAGIFLLSSVQLIKAQPATYDRNILRDPMVMKPG
                     YDRSGEGLIRSSVVTINDYDNFLLGRDFAECSITNNPLNPLQYYATWNSTSLAGGKGY
                     YTNDGYNWTAKNPDWTNMMGDFVVTYDGQGRLLYQNMYGGIDGAKVAVSTDNGATWNS
                     YVNAIVGVDKNWIAADQTDGPFANYVFNTMTANQGGNLSRSTDNGASYQNTKNFSTQN
                     LPGMMVAVGPNGAINGGSVFVVTNSGSSFSSLYTFYQSVDGGLTFTQRSQQQFSNYIG
                     VNVNGRNSVHNMRTRPYPFIAADNSAGPHRGRLYLVYASNNPSGNGKKPDIFCRYSDD
                     AAATWSSATVVNDDTQSENNYNWFPAIWCDKETGRLYISWLDTRDCPTSDSCLLYASY
                     SDDGVIFAPNVPLSQQKFRINCTTCGGGGTPVYLGDYNGVASNRYGSLMAWTDFRVGK
                     FDSYVAYFPDFAMRLEPSIDTLSSTADFLFKVPGVKHYTDTAFISAELDASAASLFNV
                     TFPDGNKLWSFPGELPISVVPAGAVPMGDYTMTITAKGTNGTPVHKRMVTIRAINPLP
                     PVCNFAVSDASACQGLPVSFQDLSSGPPTDWQWSFPGGIPETSDEKNPQGIVYNTAGV
                     YDVSLIVTNAAGTDTLVKSSFITVSEVPPAPAAAGAEVCFGQPVPDLTAQGTHLQWFF
                     NGFLVGSDTVYSPYQTQPGVYLYTVTQTVNTCASPATSVPLVIHSLPEVSFAPLDSIC
                     GNAVPFSLTEGSPAGGYYTGPGITSPDLFSATTAGAGIHSLSYIYTDEYGCIDSASRL
                     IKVLPLAEVSMDSVPSVCDGTSPFNLSGSPAGGAFSGTGVTGSQFDPAISGPGTFTLT
                     YTITDTTTGCDVWATREITVHPLPDPQLTDQSVCGNQVVLIDASSPNFIKYLWQPGGE
                     TTSAIQADTAGLGLGTFGFTVAITDINNCVNTDSVSIHFYDCTGLEEPVKSSEIEIFP
                     NPGNGLITLYSTVVPEGEYQLKVFNSANALIYSESGIPAKGEVKKQLDFRKFANGIYL
                     LRFENSESGWSRRFVIQR"
     gene            15716..16246
                     /locus_tag="PKI35_02035"
     CDS             15716..16246
                     /locus_tag="PKI35_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43187.1"
                     /translation="MKSKLIQKTGISMLLMGFVVLFSGCPFSGSVPIDNGTVAIADQL
                     NGEWIKPTDLTEENPTTYMIRQGDDKYHAVAEKSEYSTDDAEYNTTVYNLTFSNVSGE
                     TFMSAQEAGTTTYYYYKFAFNKETKEISITEVSDYIKETFNTSEELKSFIANYKQLSF
                     FFTNTTDTYQIRPSDK"
     gene            complement(16277..17089)
                     /locus_tag="PKI35_02040"
     CDS             complement(16277..17089)
                     /locus_tag="PKI35_02040"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNX43188.1"
                     /translation="MIENINETASFLKSEGITEAGTGIVLGTGLGNLVNQIAIEKSIE
                     YADIPHFPLSTVEYHRGRLIYGTLSGKKVLAMQGRFHYYEGYTMHQIVFPIRVMKALG
                     VRYLLLSNAAGAINPSFKKGDLMLLDDHINLLPANPLTGINYEKLGPRFPDMSRPYAA
                     HLNKLILSQADALNIRIHSGVYAAVPGPNLETRAEYRYLRSIGADVVGMSTVPEVITA
                     NHMGLPCAAISVLTDECDPDNLLPADINDIVKTAGLAEEKLNRLIAGVIRNL"
     gene            17280..17561
                     /locus_tag="PKI35_02045"
     CDS             17280..17561
                     /locus_tag="PKI35_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43189.1"
                     /translation="MDTDNEQSLERLRILLEKNFSWPSVYMFKLIIPADNRIFAMVKN
                     LFPEEARFFIRNSKSGKYIIITVKELMLNPEEVIARYREALEIEGIIML"
     gene            complement(17678..18217)
                     /locus_tag="PKI35_02050"
     CDS             complement(17678..18217)
                     /locus_tag="PKI35_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNX43190.1"
                     /translation="MSNRRKARRLAGEEWLPLVDEEGKITGKAPRSAVHGKPGLLHPV
                     IHLHVINRKGEIYLQKRPVNKLVQPGKWDTSVGGHISFGETVEQALEREASEEIGLKD
                     FKPQPVARYRWDTEIESELSFSFVTLYEGPFHISASEVDEGRFWTRSEIERSLGKGIF
                     TSNFEHEYSILKEVVFKAR"
     gene            complement(18233..18724)
                     /locus_tag="PKI35_02055"
                     /pseudo
     CDS             complement(18233..18724)
                     /locus_tag="PKI35_02055"
                     /inference="COORDINATES: protein motif:HMM:NF016189.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
     gene            complement(18821..20353)
                     /gene="rny"
                     /locus_tag="PKI35_02060"
     CDS             complement(18821..20353)
                     /gene="rny"
                     /locus_tag="PKI35_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNX43191.1"
                     /translation="MLIAIGVVAGGSIALILATTLLRKAIEKKSIHLLHDAEEKAEVI
                     RKEKILQAKEKFLQLKSEHEKVINERNNNVAKAENRIKQREQQLSQKLEETQKKQKEI
                     NDLQVNLEQQKEILNKKQQDIDREHKVTIEKLEAISGISAGEAKAQLTEILKDEARAE
                     AMVHINEIVEDAKLKANSEAKKIVIETIQRTAAEHAIENTVSVFNIENEEIKGRIIGR
                     EGRNIRALEALTGIEIIIDDSPDAIILSGFDPIRREVARLSLHKLVTDGRIHPARIEE
                     VVAKTKKQIEQEIIETGKRTAIDLGIHNLNHEMIRLIGKMRYRSSYGQNLLQHSREVA
                     NLCATMAAELGLNVKKAKRAGLLHDIGKVAENEAELPHAILGMKIAQQFKEQPDICNA
                     IGAHHDEVEMESLIAPIVQVCDAISGARPGARREVVEAYIQRLNKLEELALAYPGVVK
                     TYAIQAGRELRVIVGADKVSDEESNQIAYDLSKKIQEEMTYPGQIKITVIRETRAISY
                     AK"
     gene            complement(20644..20937)
                     /locus_tag="PKI35_02065"
     CDS             complement(20644..20937)
                     /locus_tag="PKI35_02065"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNX43192.1"
                     /translation="MADEIAITLTIASRPYRLFVRREEEEAIRRAAKQVEEQIGHYAE
                     NFSYNDTQDLLAMVALHFSITGQAAETEAAFTSDKLYDTLAKIDAVLTETLKD"
     gene            complement(20951..21238)
                     /locus_tag="PKI35_02070"
     CDS             complement(20951..21238)
                     /locus_tag="PKI35_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43193.1"
                     /translation="MQNLSTLVTGLEYKSRQVADKMKELQRSLDELQHKYQQLEEKHE
                     QQKQTIYDLDQKIQILKITHAIPKGRETADAKKKINELLREVDRCIDLLND"
     gene            complement(21390..21623)
                     /locus_tag="PKI35_02075"
     CDS             complement(21390..21623)
                     /locus_tag="PKI35_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43194.1"
                     /translation="MRDDGSFKMDSERTGYILSGMNCMRDDDGGFDFRTILTVPDDYY
                     IPESLKISKPVRDKRVAAMREELFKQPYELQPE"
     gene            21863..23041
                     /locus_tag="PKI35_02080"
     CDS             21863..23041
                     /locus_tag="PKI35_02080"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNX43195.1"
                     /translation="MIQQYLKMILGIFTLVFGLPESKSQDFVPVFPLNSEGYSCFRIP
                     AIINLPGGELLAFAEGRTMNCADFGNVDVVMKRSADAGKTWSPLKVLIDNDSLQAGNI
                     TPVVDRCDPRFPRGRIFLYYNTGNGQEWEIRTGKGVREVWYITSADGGNNWSKPVNIT
                     KQVHCPNQPAYNPEYNDPADWRGFANGPGHAMQIKTGKYKGRIVVPANHTEGNPQTDW
                     TDCFAQAFWSDDHGSTYHRSDKLGIPGSNEAIAAELSDGSVMINARNQKGNPRLRIVA
                     VSHDGAGSWDTAWYDSQLIDPVCQGSLLDTRFRGMHILLFCNPASQTDRQKLTIKVST
                     DNGKEWNPGLCVVEGESAYSDLVRIDRKNIGVIFEKGSDGGIYFLSVPLKMVLKGKKE
                     "
     gene            23088..24278
                     /locus_tag="PKI35_02085"
     CDS             23088..24278
                     /locus_tag="PKI35_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX43196.1"
                     /translation="MLRKILTRTILLVSFVSLFTDIASEMLYPIMPVYLRSIGFSVLL
                     IGILEGMAEATAGLSKGYFGNLSDKMAKRAPFVRWGYMLSAISKPMMAAFVYPLWIFF
                     ARTLDRLGKGVRTGARDAILSDETTPEHKGKVFGFHRALDTVGAAIGPLLALVYLYYY
                     PQQYKWLFIIAFIPGLIAVLLTLLLKDKQVSSKQRSQVGFLAYLGYWKKAGKQYKLLV
                     IGLLFFTLFNSSDAFLLLAVKNQGLSDTAMIGVYVFYNLIYALLSFPVGIMADRIGLR
                     TTLVTGLFVFAGVYGLMGFAVSVPQFIMLFGFYALYAAATEGVSKALITNMAAREETA
                     TAIGFYTSFASIFTMLASSLAGFLWFALGPRPMFLISGIGVAIAAIFLGTNRFLREKT
                     TVGN"
     gene            24410..26143
                     /locus_tag="PKI35_02090"
     CDS             24410..26143
                     /locus_tag="PKI35_02090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNX43197.1"
                     /translation="MKKTWIYLIFFLLICYLSGYSQQGIPAGYFASPLKINLEATGAF
                     AEIRANHFHSGIDFKTLKKEGIPILAVADGFVSRIKVSPVGFGNALYIDHPCGYTSVY
                     GHMLKYNNLIGNYVYNKQYEMQSFAADLFPARDQDTFWIKKGDTIGFSGNSGTSYGAH
                     LHFEMRTTITERILNPYLFGYDNTDQYYPYFDLFKVYPMDNSSSVGASDEPVQYGVRN
                     PEQGLYYLDGVDTLFLWGNFAFGVQAFDYMYNHKDRNGFYGLRMLVDNVLCFSMQCDS
                     FSFDESRNINACIDYPAYYKSGYRIVKSNRLPGNALSFFKTPVNNGVVPFTDGKAHSL
                     VFEVYDIMGRVSRLTVPVVSRQPGKMVSVTDIVDADTICRINYDIANRVMYHNLDVTI
                     PASALYENLDLTIKELPTANGLYSARYAVHNPETPLQKNYTVSIKADKVPASLADKAL
                     IVYINPGSGRRSSEGGKMERGWISAETNRFGVFSIAVDNQKPSVKLVHQKPSKAKSVK
                     VIVKDDLSGIAGYRGEINGQWALVEWDPKYRLMIYRYDQLLKTGKNVFKLTVWDKAGN
                     TAVFTTPVNKK"
     gene            26188..27717
                     /gene="guaA"
                     /locus_tag="PKI35_02095"
     CDS             26188..27717
                     /gene="guaA"
                     /locus_tag="PKI35_02095"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNX43198.1"
                     /translation="MHETILILDFGSQYTQLIARRVRELNVYCEIHPYNHFPAITGEI
                     KGVILSGSPFSVRDEGAPIPDLSGIRSKVPLLGVCYGAQYLAMSSGGEVLASKIREYG
                     RANLEYIGPGSDLMLGMSQNCQVWMSHGDTIQKVPDNFQIIAGTRDVAVAGFQVTGEK
                     TYGIQFHPEVYHSTEGAVLLRNFVVDICGCSQDWTPASFVETTIHELREKIGDDRVVL
                     GLSGGVDSTVAAVLLHRAIGKQLYCIFVDNGLLRKNEFESVLASYQHMGLNVKGVDAG
                     KQFLAALKGIMDPEYKRKAIGRTFIDVFDQEAHLVEGAKWLGQGTIYPDVIESVSVKG
                     PSATIKSHHNVGGLPAYMKLKIVEPLRSLFKDEVRKVGHTLGIDPDILHRHPFPGPGL
                     GIRILGEVTADKVKKLQEADALFIEGLKKNDLYDKVWQAGAILLPVQSVGVMGDERTY
                     ENVVALRAVGSTDGMTADWSHLPYEFLASISSEIINKVKGVNRVVYDISSKPPATIEW
                     E"
     gene            27752..29677
                     /locus_tag="PKI35_02100"
     CDS             27752..29677
                     /locus_tag="PKI35_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNX43199.1"
                     /translation="MQKKFFIFLLIAGFLFQAGFAQEAAIKRSTVVEQYKGKPYYLHF
                     VKQGETLFAIAKAYNVDVEVIKAENPDVEKEGLKVEMVLRIPYGETSGQPAAEKQKQI
                     SDKVMDTVKFQASKQQFIEHEVSKKETLYGISKQYGVTVDDLIKANPSMTELKTGMIL
                     KVPVIQAAKPTVEAEKKKEPEPPAPGVSGDGLIEVKQGQTLYSIEKQYNISEEELLRL
                     NPELKDGLKTGQTIRLRENAGIMQKGKTVLPGQEKSGKTETVSNAVSAKPFVKNSGRV
                     FEVALLLPLRLEMADSIIKTPVEKLPELNKYKTFDFFQFYAGAMIALDTLEKEGYRIN
                     FHVYDADSEKDTLKVKKALRRSEMAGMDLIIGPLYVKSFPIAARFASQHQIPIVNPLS
                     RRNSILEKNPWVYKVYPGDQDIANALALSVTNYYPGANIISVCNTDKENKVMALAFAD
                     SVKKLNSPGLTLTQVIYQNAGFSGVSKVIDPKRMNIVILFSTNRSVVPAFVSKLNAYS
                     KSGNVVLFGIPGWEDIEMETEFLMNMDYHQVVSSFVDFSAGNTKQFETAFREKYGAQP
                     LIEKDAFLGYDIVRYFLGALSAYGKDFNKFLPEYNLPGLQYNFRFKQPLPANGYENTD
                     ARILHFKDYRWVEKKTD"
     gene            29834..30508
                     /locus_tag="PKI35_02105"
     CDS             29834..30508
                     /locus_tag="PKI35_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNX43200.1"
                     /translation="MKEKLTIDKLIAEAKAFCIAESNIKNESLFGVTDGKAVGTHIEH
                     KFQEQLNAKYETTIGSSAYGIDLPSDDILTDIKVTSIKQPQSSCPFKDAKQKIFGLGY
                     NLLVFVYDKIDEPKTKTAILDFVSCSFISKERTADYTTTYRLCEMVKDKANAEDIVAY
                     LNDKNIPADEITLNKLAKQILQSPPAQGYLTISNALQWRLQYQRVVSLTESVDGISKV
                     IDKVTK"
     gene            30508..32277
                     /locus_tag="PKI35_02110"
     CDS             30508..32277
                     /locus_tag="PKI35_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNX43201.1"
                     /translation="MRIFEANISSQVCTFFKDFLINHLAFEEANQKMFDTFGINTFFD
                     NNDEFQKLKESVSDTANSIFEEPDRAEYGDFQTNRDLAFKVTKQLFEIDNSPEIVIEP
                     TCGKGNFIVASLSCFKTIKRLFGIEIYKPYVWETKFNIIDFCLSNPHINKPEINIIHA
                     SIFDFDFNKISKQYPQESLLIIGNPPWVTNSKLGSLNSSNLPQKSNFKNQSGLDAMTG
                     KGNFDIAEYITLMLFDAFQSHNGHLAFLVKNSVIKNILFDQKEKQYKVSKIEKYCIDS
                     KNEFNVSVEASLLVCQLNSNPSIECTEFDFYNRNKKLSFGWVKSKFVSNIDDYKATKN
                     IDGVCPFEWRQGIKHDCANVMELEMIRGHFINKLSEEITIEEDLVYGFLKSSDLKNTV
                     IKNTRKFTIVTQTRVGQETSYIQRLYPATYKYLKKNISSFQARKSSIYNGKPLFSIFG
                     IGEYSFKPYKVAISGLYKTFHFTLVLPQNGRPVMLDDTCYFIGFDKIEFAVYTILLLN
                     YYKTEEFLKSITFSDAKRVFTKDVLMRLDLLSISRSIPQKEIEEQIDHLNEKYCLNIN
                     ISEWGNFILEISPIKENQLTLFD"
     gene            complement(32438..32923)
                     /locus_tag="PKI35_02115"
     CDS             complement(32438..32923)
                     /locus_tag="PKI35_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43202.1"
                     /translation="MTTQPENEPICCPPFEPAPWDDKMIEWNNKLFLKSHVCTFFYMP
                     LNFDKAMRRLDKLVKQESAAVQDYLCLSDHTSKWNMDVYLAVDKTVPGAENVTLSGKF
                     YSKVYEGPFSDTGKWCKDYESIVKSKGMAVKKWYMWYTTCPKCAKKYGKNYTVIMGQV
                     E"
     gene            complement(33119..33964)
                     /locus_tag="PKI35_02120"
     CDS             complement(33119..33964)
                     /locus_tag="PKI35_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNX43203.1"
                     /translation="MIDTIRELFSYGFFRNAMLAAVMSSVACGIIGSYIVSRRMVFIS
                     GGITHASFGGIGIGYFLGLNPIIGAVVFSLLSAFSIRLASRRTEIREDSAIGIVWSLG
                     MALGIIFIFLTPGYAPNLMNYLFGSILTVSVPDLWLMLILTLITILFFGFFYHTILFI
                     SYDEEYARSHNAPVELFNYILLSLVALAIVLHIRVAGIILVISYLTIPQTTANIFVSD
                     LKRIMLLSIIISFVGSFTGLLISYYMNLPSGATIISVFVLIFILARIIKSLSLKAGIR
                     KAMKA"
     gene            complement(33957..34733)
                     /locus_tag="PKI35_02125"
     CDS             complement(33957..34733)
                     /locus_tag="PKI35_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX43204.1"
                     /translation="MTKLIQLTDVSAGYNDEVVLRDVNLEINDLDFIGVIGPNGGGKT
                     TLIKVLLGLIKPLKGTVTRSDERSGGFIGYLPQASQVDKKFPITVLDIVLSGLMSARG
                     RFYRYSKDDNIRAHQLLAQMGIGGLAHRTSRELSGGQLQRVFICRALISNPRLLILDE
                     PNTFVDNRFESDLYQRLIELNKKMAIMIVTHDVGTISYYVKTIACVNVYLHYHRSNII
                     TEQQLADYNCPLQIITHGDIPHTVLQKHEHDHQHSLPDHD"
     gene            complement(34730..35653)
                     /locus_tag="PKI35_02130"
     CDS             complement(34730..35653)
                     /locus_tag="PKI35_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNX43205.1"
                     /translation="MRTLFPFVWLACLVPLLFSCKPHKTVRQEASVVVSILPQKYLVD
                     RISGGKLECGVMIPPGGNHETFEPSPRDMEKVSKAGIYFTIGALDFEKAWVERVKAVN
                     PGLEIVNTGSGITMIEGHSHEGHASCAVDPHIWLSPDAVRIQARSICEALCRYDSSSK
                     TLYERNLDSFLHEADSADRYITSLFEPHQGKSILIYHPALAYFAREYGLNQLSIEHEG
                     KDPSPVHMREIIDLAEARRISVIFVSKEFDTRHAETIAKQINGRLVTFDPMAYNWTTN
                     IMHLADLIAQSMANQKPGDIPAKNLSSKSRP"
     gene            complement(35971..36720)
                     /locus_tag="PKI35_02135"
     CDS             complement(35971..36720)
                     /locus_tag="PKI35_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNX43206.1"
                     /translation="MRLFGLTGNPLGHSYSGEYFTRKFSDEKIAGCRYELFPVNEAAQ
                     IRKIAEDRAELEGLNVTLPFKKPVIPYLDDLDEAAKKIGAVNCIRISRRKEGIQLKGY
                     NTDAPAFRDSLLPLLDARYNRALILGTGGAAQAVQYALGQLNIGFRLVSRNPGSDRLA
                     YGQLDKAILGDYLLIINTTPLGMYPDTDSCPSIPYSSLTDRHLLYDVVYNPEMTLFLR
                     NGLQQGASVKNGLDMLRRQAELSWEIWNEHA"
     gene            complement(36723..37346)
                     /locus_tag="PKI35_02140"
     CDS             complement(36723..37346)
                     /locus_tag="PKI35_02140"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNX43207.1"
                     /translation="MGLTEWIASTAVAVIGATAYPGVFILMMLESMVFPVPSEAVMPF
                     VGFLIVDGQFTFTGAIIASTLGSITGSLISYTIGFYGGKPFVHRFGKFLLLNPHHLEV
                     SQHFFDKKGEITILISRFIPIVRHLISIPAGFGKMNLVKFSIYTIVGAGLWNSFLTYV
                     GFKLKSNWGEVMKYSHIIDLVVIVILGAALLYYLYKIALSLSKKRAN"
     gene            complement(37412..38830)
                     /locus_tag="PKI35_02145"
     CDS             complement(37412..38830)
                     /locus_tag="PKI35_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX43208.1"
                     /translation="MDYNFDEDEDYMRELLERFEKDSEGNGSSFFDSQELEDIVYHYF
                     SGSDFQSALKAIELGLKRFPSDSTFQLLKAQYLVNNDEPEKALRLLTKIQPSEPDNPD
                     ILITKAGVLSAMDKHEESIREYRKALKFVSEGLDEIYTNIAFEYENLNLYDKALVYLK
                     KAYELFPDNESVLYEIGYCFEIGSMNEQSVAFFDEVVNKNPYSGVGWYNLGMAYKSLE
                     LFEKAIEAFDFAIAIDPEFMPAYYIKAQTYESMELFRQAIRVYKQTLEYEKPDAMVYY
                     YIGDCYEQLKKYDTAIEYYRKSIELEDQLSDAWMGIGICTDELGKKQEALPFMQQAVK
                     LEPQNADYWYILADCYNDLKLADEAIEAYQHVTELDAMNPEIWLDFSDLYFKELSDID
                     KALELIEQGIDLQPSNVDFLYRRIAYLLEAGNTKEAVRELFLTLPLDYEGHKSLLEYS
                     QKARLDPSIMDVISTYVELNGL"
     gene            39015..40409
                     /gene="glmM"
                     /locus_tag="PKI35_02150"
     CDS             39015..40409
                     /gene="glmM"
                     /locus_tag="PKI35_02150"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNX43209.1"
                     /translation="MTLIKSISGIRGTIGGPQGNGLTPPDIVRFVAAYSVFIKRNNPG
                     KRIRIIVGRDARTSGFMVNQLVTGTLMGMGIDVTDIGLSTTPTVEVAVIDKQAEGGII
                     LTASHNPGHWNALKLLNDKGEFLNAADGAQVLEIADAGDFGFSEVMALGNYDTESTTI
                     ERHIQKVLALPLVDVEAIRKAGFSVVADCVNSTGGLAIPALLHALGVNTVHELYCTPD
                     GHFPHNPEPLPENLVDLSETVVDKKASVGFAVDPDVDRLAIVCENGEMFGEEYTLVSV
                     ADYILKNTPGNTVSNLSSTRALRDVTEKYGGNYLASAVGEVNVVEKMKETGAVIGGEG
                     NGGVIYPELHYGRDALVGIALFLTHLAKSGKKCSELRDAYPSYFISKNKITLEEGMDV
                     DAIIRSIENKYEKQPKITVDGLKIEFDKEWVHLRKSNTEPIIRIYSESDSLAKADTLA
                     RKIMNDIGDIVTGR"
     gene            40531..40833
                     /locus_tag="PKI35_02155"
     CDS             40531..40833
                     /locus_tag="PKI35_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43210.1"
                     /translation="MDFHDIPGICPGQNFRIMASAKKKKFRKAKFYKVEFKIPEALHK
                     QVKKYCKKNGITPNKFHRRAIREYIELHAKYPEHREDAVGPNQMSIFDIIENNKEE"
     gene            complement(41042..43192)
                     /locus_tag="PKI35_02160"
     CDS             complement(41042..43192)
                     /locus_tag="PKI35_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX43211.1"
                     /translation="MRKEIFFVALLIFMSGAIFGQQRTTDSTKVLQLKEVIVTANRLP
                     VTLKRNPGAMSLVTPRMLSIMPKGIGAEEALRLVPGVRIDNQHDGERVHLSIRGQGIL
                     TERGLRGIGVLIDGIPVNDPSGFTPDLYDVDWSTVQKIEVLRGPAAGLYGGGSAAGVI
                     NITTQNGGPNPVGGEVSQSLGSNGFAKSLIQLNGSKQSLDYRISYSRTGGDGYRDHQA
                     FWSNKLSEKVTFHPSKKLSVTQIISHTDYFQQNPEGLNLGQFNDLRQANPDACPFNEY
                     QKTNRTTAGIHATYKLGEKQEIEATSFLRSWRYKETSNRCAEYRSITNPGLSAQYNLH
                     MAVNNLKNTFSVGTDFKWQHIDMYKLQSMSDPDRVESTDETNLENDSLLANQIIYQRS
                     SGIFVLDKIEFGKFSLIANFRYDDMNNELTNKMYAPDTAKTTKNFSRTSFRIGASYGF
                     TDELNLFANFSQGFMPPSTEELANNPVGYSGFNTYLVPATSNCIEAGIRGFLGEKLNY
                     EATFFSMKTENDFFRFKQSGRGNQEVFYGNAGNSDRIGIETYLSYAILKNLTLQLSYT
                     YADYKYTSADVDPVYTDTAYVLTLPPASGQWLPNSPKHQLYAEVTYELNHHLQFSLAT
                     EYQSKWAIYTDAKAYSGEMDPAIYQNWQDGFNLYHARIAYLWNFWKLNGECSLYARNF
                     TGEKYMAFTEPDPDGNSYQPGPGREFLANIKINF"
     gene            complement(43475..43981)
                     /locus_tag="PKI35_02165"
     CDS             complement(43475..43981)
                     /locus_tag="PKI35_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43212.1"
                     /translation="MKKSISILLVVFFLFNAGGGYYLFRIFQTRIRWEVAGEIRKAKD
                     ERERVTLVFPLDFQKGIMWIRPGREFAYKGQMYDVISSATRDQHKYYYCYNDTKEKQL
                     IAGFHKSHTSKKETEKRRKTQYNQKYLPQRPVLAFAFPFADFIYTGLGFHYISQVLIT
                     PSPPPKIS"
     gene            complement(44249..44668)
                     /locus_tag="PKI35_02170"
     CDS             complement(44249..44668)
                     /locus_tag="PKI35_02170"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNX43213.1"
                     /translation="MIKKITVVLSVLALTAFTIGITYKDGHYRGVSRASYTNEPYYGF
                     TRIDVKNGKIIHVDFFIRDSVKHEYFNQKYEKHFAGNPEYIQQCRNDWKGVRAYPDSL
                     LKYQDVNKIDAISGATWSFNIFKASVKDALSKAGSRP"
     gene            complement(45286..46473)
                     /locus_tag="PKI35_02175"
     CDS             complement(45286..46473)
                     /locus_tag="PKI35_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNX43214.1"
                     /translation="MKSLFTVITPLLVASFFTNALCVYAQEKDTVQPEKYIFTEAVRL
                     KTTPVKDQYASGTCWSYAATSFIESELMRITGDTLDLSEMFFVRKCYEQKARDFVRRH
                     GTTNFSQGGQAHDVMNTVAASGLMTEAGYPGLANGQTKPMHSEMEAVLKGMLDALVKN
                     PNGKLSPDWFNAISAVLDVYLGPLPATGSPVKGAKKINPADYIELTSYTHHPFYQPFI
                     LEIPDNWSSGAYYNVPIDDLIATLNHALKNGYTVVWDGDVSDKGFSHKNGVAIVPDVK
                     LESASNTERSKWENIPEKERQKQFYSFDKPVPQKAVTQEMRQAAFDNFSATDDHLMHI
                     TGITRDQDSTVYYITKNSWAADSNESGGYLNMSEAYVRLNTIAIMVHKDAVPKEIKKK
                     LGF"
     gene            complement(46717..47508)
                     /locus_tag="PKI35_02180"
     CDS             complement(46717..47508)
                     /locus_tag="PKI35_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43215.1"
                     /translation="MKIQYPVILIFLLLSPALSPAQDMTDYDTIAEGLLYRKEMAASL
                     SLHTLGYGLGFRLGQNKTYFKKRMLEFDLLEMKSPKETKIYNEYIPNARQYVYGKENN
                     VYILRAGFGQQKLLNRKPYWGGVEVRFFCYGGPTLSLAKPVYLYIAYYTQQDMFTYYD
                     LQLEKYDPEKHFPYRGYYNNADCDIAGRGPFLEGFSDIKLYPGLYAKSGFSFEFGEYN
                     QRIKALETGIIVDFLPKAIPIMAFKDPYHYFVTAYISFSLGKRYN"
     gene            complement(47596..47721)
                     /locus_tag="PKI35_02185"
     CDS             complement(47596..47721)
                     /locus_tag="PKI35_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43216.1"
                     /translation="MKKIAALLATVVILSILLSSCSTSEQCAAYGEASRYRVESR"
     gene            47846..48526
                     /gene="ung"
                     /locus_tag="PKI35_02190"
     CDS             47846..48526
                     /gene="ung"
                     /locus_tag="PKI35_02190"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNX43217.1"
                     /translation="MNPRIEEGWRDALYDEFQKDYFIALKQFLIEEKSMYRVYPPGDR
                     IFAAFDYTPFEKVKVVLLGQDPYHGDGQAHGLCFSVPDGVRQPPSLVNIFQELNNDLG
                     IAPPNHGNLEKWARQGVLLLNATITVRANMAGSHQNKGWEIFTDTVIKALSSRNDRLV
                     FLLWGNYAQAKEALIDTTKHAVLKAAHPSPFSAYRGFFGCRHFSKTNELLENFGKTPI
                     DWNLNELS"
     gene            48536..49558
                     /gene="amrS"
                     /locus_tag="PKI35_02195"
     CDS             48536..49558
                     /gene="amrS"
                     /locus_tag="PKI35_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNX43218.1"
                     /translation="MKVSAWFENAAGSAVKCTLCPHRCVIQEGKSGICHVRINKGGSL
                     YSENFGMLSAIHLDPIEKKPLYHYYPGSQILSIGSVGCNLGCLFCQNCEISQVPSVHA
                     VMGLKKYEPIQVTDLALQYKDNIGIAYTYNEPAVWYEFMLETAMLAAAKGLKNVMVTN
                     GFINREPLTGLLDVMDAFSVDMKGFTNDFYRKITSSSLQPVKEAIQMISSSGRHLELV
                     NLVIPGLNDDRSDFREMVKWISGELGKTTVLHLSRYFPMHKLSIEATPPSLLDELHEI
                     ASQYLDFVYVGNVSSEFSNTYCPACSSLTVLRKGYSIYLTGLDDKGRCARCNNQIIKH
                     IKQGMA"
     gene            49697..50656
                     /locus_tag="PKI35_02200"
     CDS             49697..50656
                     /locus_tag="PKI35_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNX43219.1"
                     /translation="MAINKTTLIRSIAILTLACIFLQRGGYVSAQELSEDPAGGDDDS
                     TIMAPMITPFSSSVLPEDLDYYPLNEWYPSWDNQYVRLYHNIYSYKGDSIYLELEPEE
                     SGCFTPPVKGQLVSPFGYRGRRIHAGVDLMLNYRDTVVAAFPGVVRMARYFSGYGNCV
                     VIRHYNGLETLYGHLSKISVSVNQKVNSGELIGLGGSTGRATCNHLHFETRFMGDAFN
                     PKQLIDFDHHSLIRNDLWITPSVFGRSRDYLPSDEPLAKQEPVAATSHKAIKTKAKYH
                     KIKSGDTLYSIANRNGTTVKKLCSLNHMKQSKVLKPGSRIRVK"
     gene            50805..52058
                     /locus_tag="PKI35_02205"
     CDS             50805..52058
                     /locus_tag="PKI35_02205"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNX43220.1"
                     /translation="MRPTRKILLFLLLIIIIQACKPVNEIAVRNLSWKYQGMSASPDI
                     RCTLVNVNDSVSRVFIAIPPQLPDNRFDTREDSAKAGYRIKYVLYNSWLSAAVADSGT
                     VFYEFTRSRADDKPLKAEFNLRAPVGNDYFLYLKVLDIQHEKESEALKGLLKRNEGTA
                     DWFMVNDAGGELMNQRYLSNNSPVGVRCYNPAQEKFYVKVFRHVFQPALPPFVMEMRE
                     PYAENADSIFTILPSLENTASLSLYDQGIYHIQNDSSQTDGLSLVRFYDGYPAVTSPL
                     RMIEAVRYITTSKEYDHLMQSSDTKAAIDSFWIATGGNLSRGLELIRKYYGRVEQANR
                     LFPSCCEGWKTDMGMVYIIFGPPNVVYFNGNQEEWIYGEARNYHSVHFVFSKVVNPFS
                     DEDYRLQRQPNYKEFWYMAVQQWRR"
     gene            52340..53113
                     /gene="rlmB"
                     /locus_tag="PKI35_02210"
     CDS             52340..53113
                     /gene="rlmB"
                     /locus_tag="PKI35_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNX43221.1"
                     /translation="MAYYQNNPSRISQPENMIYGLRPVIEALKAGKEFDRILLQRDAR
                     NEQVKELIKLARESGVLMQQVPAEKLDRLTRSNHQGVVGFMAVISYQAIEDVLMDVFE
                     KGKTPLIVVLDRVTDVRNMGAIARSAECAGVDALVIPSRGGAQINADAVKTSAGALNT
                     IPVHRSDNLKDTMQYLKQSGLTLVAASEHARDNYYDADLTVPLAIIMGSEEDGVSPEY
                     LKLADAKVKIPLYGSISSLNVSVAAGILLFEAVKQRNKI"
     gene            53415..55316
                     /locus_tag="PKI35_02215"
     CDS             53415..55316
                     /locus_tag="PKI35_02215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNX43222.1"
                     /translation="MLLIIIIGAILRFYDYSGFSFSNDELSAINRLRFSTFRDLVEKG
                     FYVDGHPGGIQVMLWYWVKIFGVSEASLRFPFVIFGILTVWFSYLTSRQFFGAVTGLF
                     VSAAVAFLEFPLVYSQIARPYGSGIFFCMLMVWFWYRVVNGFQEKTEIKKKRLLNLAG
                     YTISTALCMYNHYFSFLLAGVVGITGIFLIKRERLTGYLAAGLIACLLFLPHVPITLN
                     HLTYKGVGLWLGKPSPQWIFGHILFIFNEQLWFLILTGMVAATLALINRQRSGLYKYQ
                     TVSFLFFILPMVMGYLYSVAVNPVLQHPVLLFSFPFLLFLIFSCAGDNLKPIHSLLLA
                     FFLTAGIYSTVGCGHYYRKQHFGEFKDVAARIAATGNRFGKNNVTNVVSTNCPYYISY
                     YYAKQGDSARLSMTEVTADSLESLGRLVRNCGTPYFLYAWTKPSPPEADDIIMASFPY
                     IIDEKDYGGLSSLKVFSVNKPEKGYTESRVIFFRQGLPEDKMRKSPDRNFHNSLPSGG
                     AGLAVIDSTEEYGPALTVPASEIRATGQETVRAEASVYMFKTPTQAVFVTSLEVPGKE
                     PLIWKGALVRNYADAGKWQKVVQTFLLPEKIDKSSVLKIYIWNKGKESLLVREMNLSV
                     VANENASDD"
     gene            55333..>55847
                     /locus_tag="PKI35_02220"
     CDS             55333..>55847
                     /locus_tag="PKI35_02220"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HNX43223.1"
                     /translation="MKKIVMNRPAFLFCLLAVVAMGCAKTKTDYYPNGSVMSVRHFSG
                     RKLQGISVWFYENGAKQQEVSYRHDKPEGLTTRWFANGNKQSEEMYREGKKNGKSVMW
                     DENGNKVEERNYRNDTLNGQYSFWYSTGMVKVTGNFSKGLYDGKWEYFSETGVKVGEG
                     NYEKGSGILTG"
BASE COUNT        15124 a        12154 c        13476 g        15093 t
ORIGIN      
        1 cctttaaaga attataccgc gtaaataatg gacgcacaac cccttgcaaa atgccggcgc
       61 cgtattgccg gttcccgcta gtaattacaa cacctgtttc tgttcgcaat tcccataaca
      121 tcaccctatt cctttgtact ttagcgcccc cataaaacag gtaaactatg gataatactg
      181 ccgacaattt cgtctcaggt ttgcacgggt ggcacagtaa agccctcggt gaaaaaaccg
      241 cggagagcct ccgcaaaaat aattttcgcg ccgaatacct tgaactgcct gaagaggccc
      301 ggaaccgcat tctcagtcag attaatcccg gcgacaggat agccttcggc gggtcgtaca
      361 ccgtgaagca gttagggctt ccggaattga tagaaaaaat tccggtcgtt atccttgatc
      421 acaatgctcc cggacttgat gcagcgcaga aacttgatat catgcgtcag cagcaggttt
      481 gcgacgtgtt tatctgttcg gccaacgcta tcacccttga tggacatatt tttaacgtag
      541 acggaacctg caaccggata tcggctatgg tcttcggccc caagaaggtg atcgtggtgg
      601 caggcgtcaa taaaatctgt caggatgaag cagccgcctg ggaacgcatc cgcatgattg
      661 cagctccggt gaacatgaaa cgcctcaacc gccgcacgcc ctgtaccgtc acgggttatt
      721 gtacagattg cgacagccct gagcgcggtt gcaatgctta tcttgtcctg aaaaagaagc
      781 ccgctttggc tgatttttcg gtttttattg ttaacaaatc gctgggtttc tgattgttgg
      841 caaattgttt agaaagtact tgcctatatc gtctgtggtt ttatactttt accgcctcat
      901 tttcattcaa acaatcaggc atggaacttt cccctatcaa tgcgatttca ccggtcgacg
      961 gccgttaccg aaaacaggtc gaaaacctgt cctattattt cagcgaagca gcgctgatca
     1021 actatcgggt ttttgttgag gttgaatact ttgttgcatt atgcaacctt ccgttgccgc
     1081 aactggccga agtcgacaag aaaatgatat cgcaaatcaa gtccgtttac cggaatttta
     1141 cctttaacga cgcccaggaa gtaaaggaaa ttgaagcagt gactaaccac gacgtaaaag
     1201 cggttgaata cttcctgaaa aagcagctcg acaaggccgg actgggcaaa taccgcgagt
     1261 tcgtccattt cgggcttact tcgcaggata tcaacaatac ggctatgcct tatgccctga
     1321 aacaggcgat gaacgaagtg gtgctgccca tagttgagag cctcgttgaa aagctgctga
     1381 aaaagtcgaa agaatggcag aaagtttcca tgctcgctcg cacacacggg caaccggctt
     1441 cgccaaccct gttgggaaaa gaaatgtatg tttttgttga aaggctgaaa aagcaactga
     1501 aactcctgaa agctgttccg tttgaagcaa aattcggcgg cgccacaggc aacatgaacg
     1561 cccactatat tgcctatccc gacgtcgact ggattgcctt tgccgacaca tttgtcaaaa
     1621 aacaacttgg tcttgagcgg caacacacca cgacacagat tgagcattac gattcgatgg
     1681 cggcgctgtt cgatgcttta aaacggatca ataccattct gattaatttc agccgggata
     1741 tctggagcta catttcgatg gagtatttcc ggcagaagat aacggccggc gaggtgggct
     1801 cctctaccat gccgcataaa gtgaatccca tcgattttga aaatgcagaa ggtaatcttg
     1861 ccattgccaa tgccattttt gaatttctct cgtccaaact gccggtatcg cgtctgcaac
     1921 gcgatcttac cgattcaacg gtttcgcgaa atatcggcgt tccgctggcg cacatggtta
     1981 ttgccctgaa atcgttgctc aaaggtatcg acaagctgat cctgaatgaa gcaaaacttc
     2041 agtccgacct ggaagataat tgggctgtcg tgtccgaagc catccagacc atcctccgcc
     2101 gcgaagggtt tcccaagccc tatgaggcgc tgaaagagct tacacgcacc aataagcaca
     2161 ttgacaaaaa agcgatcagc gattttatcg atggccttaa cctgccggca gcggttaaaa
     2221 aggaactgca taaggtcacc ccgtcgaatt acaccggtaa attcgaaata cgggaaaaat
     2281 gatgccggaa acggcaatag acatcatcga agtatttttg tacttttgca gaaaaattga
     2341 actactccat ggatttgaaa gattatttag ccatttcggg caaacctggc ctgtacaaaa
     2401 cagtggccca atcgaaaaca ggtgtcattg ttgaatcatt aatcgacgga aagcggttac
     2461 aggcatttgc cagcgataaa atcagttcgt tgggcgagat cagcatttac acagatgggg
     2521 atgatatgcc ggttcgcgaa gtcttcaggc tgatgtatga aaagaaggac gaagttaaac
     2581 tgcctgacct taaaggcggc gataaagata taaggaccta ttttgaaacg gtgatgccac
     2641 aattcgaccg cgaccgtttc tatatctccc atatgcgtaa ggttatcagt tggttcatcc
     2701 tgctgatatc gaaagatatc accgattttt cggcgccggc cgaagaaact tccccgggtg
     2761 aagaaaaaga ggatacgccg ccggctgaac caaaagacac tgaataagga tgagtccgca
     2821 taaatggcgg actttttttt acctatttta gcaaaaaaat cagtcacttc tccatgacga
     2881 aatatatcca cgatttcagg attatacaga acaggcaggt gaatccgggc tgtcacgttt
     2941 tagaactcct gtgtcctgta aagttgcctg aaatacaacc ggggcagttt gcccaattac
     3001 tcgtcgaaaa gtcgccggca acctatctgc ggcgcccgat ttctatttat gatgccgatc
     3061 cggaaaagaa taccttgtcg ctgatgataa aagtagttgg ggaagggacg gcttcgctgg
     3121 ttaaactggg ggagggagac agcctgaacc ttgtttatcc gctgggtaac tcattttcgg
     3181 tgcctgaagg taaaaaggtg ctgctggtcg ggggcggcgt gggtattgcg ccgatgctgc
     3241 tgcttgctaa acggctgaag gctgaaggca aagagattac catcctgatt ggcggtcggt
     3301 cgtttcagga tatcctggaa gctgaacgct atgaagccta cggttcggtg gggatcacga
     3361 cagatgacgg ttccctgggc ttcaaagggc ttgtaaccga acatccggct tttgctgctg
     3421 ccggagaata cgccatgatc tatgcctgcg ggcctgatcc catgatgaaa gccgtagccc
     3481 gtattgcaca caaaatgggg gcaccctgcg aggtttcgct cgaaaacctt atggcttgcg
     3541 gtatcggggc ctgcctttgc tgcgtggccg aaaccactac cggcaaccgg acaacctgcg
     3601 tcgacgggcc ggtttttaac tcaggagatt tagtgggatg ggaatgatca cgaactacga
     3661 attccacgaa cgtaatcacg aattccacga atcacaaatc atgaaactca aaactcagaa
     3721 ctcaaaactc agaactcaaa acgccaaagg ccaagagcta atagctaata gctaacagct
     3781 aacagtttac ctgcctgggc gaggttgagg gtaagacgtc aggccaaaca caaaacttaa
     3841 aatccgcgaa tcacgaataa cgaatcatta tatccctggt aaatggcaga tctttcggtc
     3901 gacataaaca aactccatct gaaaaatccg gtaatgcccg cttcgggaac atacggctac
     3961 ggtcctgaat ttgacgactt ttataaggtg agccgtctgg gcgcaatcat tacaaaggcc
     4021 tgcacttcga aacaccgtga aggcaaccgc tatccccgca tggcagaaac gcctatggga
     4081 atgttaaatt cagtcgggtt gcagaacaaa ggggtggact acttcatcag ctccatttat
     4141 cctgaaatca gtcattacga taccaatatc attgtaaacg taagcgattc aactattgac
     4201 ggctatgttg aagtagccga aaagctcaac aggcttgaaa aggtttccgc cattgaagta
     4261 aatatttcct gtccgaatgt gaaagaaggc ggtatggcat tcggaacttc ctgccagatg
     4321 gccgggttgg taacgaaagc ggttcggaaa gcctatgata aaaccctgat cgtaaaacta
     4381 tcgcccaatg tgaccagtat tgccgatata gccctggcgg ttgaagccga aggcgccgat
     4441 tcggtttcac tgatcaatac gttgcttgga atggccatcg atgctgaaaa ccggcgcccg
     4501 cttctttcaa cggtcaccgg gggattgtcg gggccggccg taaaaccggt tgccctgcgt
     4561 atggtatggc aggtcgccag ggcggtgaaa gttcccgtga taggaatggg cggtatctcc
     4621 aatgcatccg atgccatcga attcctgctg gccggcgcca ccgccattga aatcggtaca
     4681 gccaacttcg tcaatccgat ggtttgcaac caggttattg acggaattga agcgtacctc
     4741 gacaggcacc attgcaaatc ggtaaaagaa attatcggcg cactccgggt gtaaaaatgt
     4801 cagttccctt attttgagat acacaatgca gaaaaagaat gttttaataa tcggatcggg
     4861 cggacgggag catgcccttt cgtggaagat agcccaaagc cctttactca acaggctgtt
     4921 tatcgccccc ggcaacgccg ggacaggctt cacggggatc aacatcccgg taagcgggaa
     4981 cgattttgag gctgtgaaac gcgccgttct tgaaaatgat attgaaatag tggtggtagg
     5041 ccccgaagat ccgctggtaa acggcattgc cgatttcttt gccagcgacg ggttgctgaa
     5101 ggatatcctg gttatcggtc ccgggaaagc aggggctatg ctcgaaggaa gcaaggactt
     5161 cgccaaaggg tttatgcggc gtcataaaat cccgacggct gcttacgaaa cgtttacggc
     5221 tgaaaccatt gacgaaggat tggctttcct gaaaaagctt tcgccgccct atgtattaaa
     5281 agccgatgga ttggctgccg gcaagggtgt tctgatatgc cgttcactcg gagaggcttc
     5341 atcggaactt aagtccatgc tggttgattc caaattcgga aaggcatcct caaaagttgt
     5401 catagaagaa ttcctggatg gaattgagct ttccgttttc gtactcacgg atggtgaatc
     5461 ctataaaata ctgccggaag ccaaagacta taaaagagcc ggtgaaggcg ataccggacc
     5521 taatacgggc ggcatgggct ctgtttcccc ggttccgttt gccagcggcg acttcatgca
     5581 aaaagtggaa gaacggatca tcagaccgac tattcagggc ctcagggaag aaaatattcc
     5641 ttacaaaggc tttattttct tcgggcttat gaatgtaaag ggcgatccgt ttgtgattga
     5701 gtacaatgta aggatggggg acccggagac agaagcggtt atgccaagga ttaaaagcga
     5761 tttgcttgaa ctgatgatcg ctatagacga agacagattg tccggcttca accttgaaac
     5821 ctatcccgaa acagccgtgg cagtaatgat ggtttcggga ggttacccgg gagcatatca
     5881 aaaaggattg accatcgatg gtctccgaaa tgaggaactg ccggtgatat ttcacgcagg
     5941 aaccacttac gatgaatcca caaaggatgt tgtaacctca gggggaaggg tgctggcggt
     6001 tacttcgatg ggcgtaactc tgaaagacgc cctccgtaaa gcttacgccg gcgcagagaa
     6061 gattaagttc aaggattcct gctaccgccg cgatatagga aaagatttac tttagccatc
     6121 tcattacctc ctggaaatgc tgaagcgatt atcaaaatcg gggtttttac tgcagtttat
     6181 cctgtttctg gctcttggga ctctattttg gttcccggca ttcatgcatc ccaagccggc
     6241 ttcgatgatt gaatctgccg gaccgttgta caggatgatt gccggatggc tgggcggcga
     6301 cgcctcgttt tcggtgatat tggccgcggt tctcctggtt gccgaagctt ttgcacttca
     6361 tgcgctgttt tcttctcatg atcttattcc ccgcgacagt tttattgacg ccatcatttt
     6421 tctgatttgc atgagctgga acccggagtt gttgtattta aatccggcga taacagccgg
     6481 catttttatc ctgtttgccc tttttatgat gatgaggatg tacggccgta ccgaaccgtt
     6541 tcaggaaata ttatcggcat ctttatctat tgccgttgcc tcgcttattt ttcaacctgc
     6601 ttcctggttt tttatcgggt tatggatagg gcttctcaca tacaggatta cctcatggcg
     6661 cgagtgggtg atttccctga tcggaatggc gctgccgtta atctatatgg tcagtattta
     6721 ttatctgcaa gggactttaa atgaaaattg ggttttcctg atagcaccat tccaagaaat
     6781 ttcgatagac atagcgccat tctcttctgg cgaaatctcc tttctggcag cagctttgtt
     6841 tatgcttttt tatgcttcgg cagcaacttt aaattccgta tcggataaac tgataagtat
     6901 caggcgcaag acgtggatca ttaccgattt ttcccttgca ggcctttttt cgatactggt
     6961 tgcccattcc gcgatgaaaa tcggtcagtt gctgctgatg gccccgctgg cgttcttcct
     7021 gacctatgcg gccatctcaa tcaaaaagag caggttcaat aattttatca ttgtgatatt
     7081 cttattgttt gtcatcgtga tacattatat tttgtaaatc atgcttctgc gccattatca
     7141 ggataaactg gttgaagcag gcgttgatga agccggaagg ggttgccttg ccgggcctgt
     7201 ttatgcagcg gctgtaattt tacctcccgg ttttgtcaat gagaggctga atgattcaaa
     7261 gaaactcacc gccaggcagc gtgatgaact gaggaccatc attgaaaagg aagctttaag
     7321 ctggtcggtt gccgctgccg accagaacga gattgaccgg atcaacatac tgaatgcttc
     7381 attcctggca atgcaccgtg ctattgaggc tttggatcat aagccggaac tcctgcttat
     7441 cgacgggaac cgttttcggc cgtatccgga aatcagccac cagtgttttg tgaaaggtga
     7501 cgcaacatat atgtcgattg ccgctgcctc tgtcctggcc aaaacccacc gtgacgaatt
     7561 catggcttca attgccgggg attacccgga gtacggctgg gagcagaaca aaggctaccc
     7621 gacagccttt cacagagatg ctatcgtccg gcatggcata tcgccattcc atagaaaaag
     7681 cttttcattt cagcaaaagt tgttctgaag tcgctatttc cgattactca gggttattaa
     7741 gtcatcgcat gccggaatgc ggatcgggag ttcaggaacg ccaggtatgg tccttttgca
     7801 ggagcgaagg ggctgtctct tattcaaaac ggattgtaaa tcattttctc agaccggtct
     7861 ccggatgaca gaaataaccc ggaaggaaag gcgcctggcc ggaactcatc cggttaacaa
     7921 tcattcccag aattttaaag atctgtaaat caacgtgaca cataaaacac aaaaccccgc
     7981 cttgagagcg gggattcatg aaccaaatta ttaaccaatt ttaaccagaa aacctctaac
     8041 tagagaaata tgaaaattag tcctttaata ttttttttcg ctttttgatt cagcgaatca
     8101 ttcattctta atttaacgag gcaaaattag tattatttta tatatcaaac gacgagttaa
     8161 taaaaaaaat tattaattat ttaatacttt gctgtatgaa gtataatttt atccttgaca
     8221 aaataataca aatatttatc tgataattat ttacagattt ttttcggggt taaaaagctt
     8281 agtaattccg gccggaataa ttttaaggct ttttgggcta ttcttttaat cattgagccg
     8341 gaatcttttc gatacaaaat ttatctttgg actttaataa tctcttctgg aaaatcatga
     8401 atttaaaaaa gcctcttttt ataacagcct tattactagt tgtatgcctg aatactattg
     8461 cccaaggtaa accagcgcct cccgattatg cggcgtatat taataaatac agtgccctcg
     8521 cattgcgtga aatgcagctt taccgcattc cggccagcat caccctgtcg caggggatca
     8581 tcgaaagcaa ctgtgggaaa agtccgttag cgctgcaggc caacaatcat ttcggtatta
     8641 aatgccagaa agactggacc ggtgaaacgt ttttaatgga cgatgacgaa gaacaggagt
     8701 gtttccgcaa gtataacaat gctgaggagt cgttcaggga ccattcgctt tttcttacta
     8761 cccggcagcg ttatgccgtg ctgttcagtc tggatatgac agattataaa ggctgggcaa
     8821 agggactgag caagtgcggt tacgctacaa atccaaacta tcccgagatc ctgatccgga
     8881 tcattgaaga aaacaagcta taccgctttg atgatacgct ggtagtggcc cgttcagaca
     8941 gaaatgagga gaacgacgac cggaagccag aaaaagaaga gtttaaggaa tacagaggcg
     9001 gtcaacttac cgtgttcttt catgatggtt ataaaatgcc gtctccggct gattttactt
     9061 tacgtgaggt gagtaaactg gggagaaaag tatacgaaaa taatggtgtt ccctttattt
     9121 ttgcactgaa gaacgatacc tggcagtccc tggcttcgga gtttgggatt tataccttcc
     9181 aggtctataa gcaaaacgac ctgaaacaga ccgacgccat tacagaagga cagatgcttt
     9241 accttgaacc gaagaaaagg aaaggcgcgg tgccggcttg tattgtaggg ccttatgaca
     9301 gcatgtattc catagcgcag cattttggcg tccaattgaa atttctttac aagtacaacg
     9361 ctatgctgcc cggtgatgaa ccccaaaaag gctccaccat ttatctcaga aaaaaatagt
     9421 ccgggttttg ttggttatat accgaattct ttaattttgc acccggttgt taacctaata
     9481 acaatcaggc attcttcagg gcagggtgaa attcccgatc ggcggtaaag tccgcgactc
     9541 ctttcaaaga ggactgacat ggtgaaaatc cgtgaccgac agtaaagtct ggatagaaga
     9601 aggatgacag cagggtgcaa tggtttatag tataaatcgt tgaaagcccg gttgctgtat
     9661 ttcttttctc gcgctgccct ggtactgttt gccggggtat ttttttaaca aatagcgtca
     9721 tgagaaaaat caaactcttc ctgatctgcc tggcctcttt aatttgtaca aacaaccttc
     9781 tttttggcca ggaaaaaggc gaaatcaccg gaacggtatt ggatcaaagc actatgaggc
     9841 ctgttgaagg ggtgtcggtc gacattcctg aaataatgca gggaagcatc accgattctt
     9901 ccggaaggtt tctctttaaa gcggatgccg gcacatacag gatacagata tcccacctct
     9961 cctacgaggg ggtgaaccgt aaagttgtga tcagcggggc caaagctact gatatgggaa
    10021 ctattttcct gaatccggtt gtagtttcgc tcaaagaagt taaaataatt tcttcctata
    10081 tctctgaccg gaatacgccg gtttcagtta ccacgcttgc ttcggctcag attgaaaaac
    10141 aaaccggtaa caaggattat cccgaactcc tcaatctgac tccgggcatt tatgccacaa
    10201 aacagggcgg cggttccggg gatgacaggc tcaccctgcg cggctttcag caggagaatg
    10261 tagcgctttt gctgaacggt gttccggtca gcagtatgga gaatggcatg gtgtactggt
    10321 caaactggat ggggctgacc gatgccaccg aagcaattca ggtacagcgg gggctgggcg
    10381 catctaaagt ggctatgaac tcggtaggag gaacggtaaa tattattacg aaaagctctt
    10441 cttccgaaaa aggcggtttc ctgcgttata gtatttctga tttcgggaac cagaaaacct
    10501 cgctgcaatt gagcacagga aaaatgaaaa acggcttgtc gctgacattt ctcggttccc
    10561 gcacgacggg ccccggctat gtggatggaa cttatgtaaa cggctgggct tactttctat
    10621 cgttgagtta tgacgccata ccgcgtcaca aatttgtctt tacggccatg ggatctcctg
    10681 aaaaacacgg ccagaaaaat tacggaagtt cggttgccga tttcgagaag tacgggaata
    10741 agtacaactc cgagtggggc atctataacg gtaaagtcct gaacctgtcg gaaaatttct
    10801 atcacaagcc acaaatcagc ctgaatcatt actggaatat aaatgaaaaa atgttgctgg
    10861 cttcttcggc ctatgtttcg ttcggatacg gcggcggacg ctattcggaa gcctataact
    10921 acggaaccgc cacttactat ttccgtaaaa acaaccaggt ggattttgat gcggtatatc
    10981 tcaacaatat caacaattct gattccgtgc aactggccga cggttctacc gtaaccggct
    11041 attcgaaaaa catcctgacc cattacaggg caaatcacta ttgggccggc gtgctatcga
    11101 ccctgcatta tgacctggcc ccaggtgtta aactcatggc tggtattcat ggccggacat
    11161 tccgctcccg tctgtatgaa gaggttaatg accttatggg cggtgaatgc tggattgaac
    11221 aatatgcctg gtcgctggcc ggggttgccg gaaggcggca ggttaaaaag atcggtgatg
    11281 taatcaatgt tgataattac tcgctgatgc aatacggaaa tatttttgtt caggctgatt
    11341 atacgaaggg gaagtttaat ctttttctgg catctacggc ttcgggaact caataccaac
    11401 ggaaggatcc ttataattac cctgctgatc catacagcag gaaagtcttc aaacagggat
    11461 tcgatacgaa agcaggcgtt aattattccc tgaacaaaaa cactgacttt tatctgaata
    11521 taggctatta ttcccgcgaa ccgctctata aattcgtata tatcaactat tccaatgctg
    11581 ttgcaaaaaa tctgaacaac gaaaagatac gggcagccga actgggatac tccttcacca
    11641 acggggatac atttttacgg atcaacggtt atgtgactga ctggcgtgat aaatcactgc
    11701 tttcgaaaga atatattcag ttggccgaca gtacgttaaa ccgtacgctg gtccgcgggc
    11761 tgaatgcgct gcatatggga atagagcttg aagcagcaac ctcgccgttc cgtaatttga
    11821 aaacgggatt aaccctttcg ctcggcaatt ggaaatggac caatgatgtt acagccagca
    11881 tttacgacga caaccaggtg cttgtagata gcctgaaggt ttatgccaaa ggtttatatg
    11941 taggcgatgc accgcagacg cagcttggcc tatacctgga atattccttc tggaacggat
    12001 ttgacctcac ggtaaactgg atatattatg acagattgta cgccaatttt gacccggcaa
    12061 acagggtgac cccgggcgac aggtcgcaac cttaccggtt acctgcttat ggaatgaccg
    12121 atatctgcct cggatacaat ttttccgtca ggcaaatacc atcccggctt cagatcactt
    12181 gccagaatgt gtttaataag gttgcaatgc tgaggggaga cgacggcgct gatcacagcc
    12241 tggacacctt tactggtttc tggtcaatgg gaaggacgtt cggtatctcg atgaaggtga
    12301 tgttctaaaa ccgatgcgcg tttttattgg taacagatga gccggacaat gttttcccgg
    12361 ccgtaacctg ataacctgaa gcaatcacaa atgaccggat ttgtaaacca tccggctttg
    12421 ttatcacctt tgaatcacaa accgtctgct ccatccggac tcagaattct cgaaacggag
    12481 gaggtaaatg ccgttggcaa atttccggaa atcaagttgt ttcttaactt ctcctttggc
    12541 aggaatgccg gattcggaat aaataagcgc attcgctgaa ttgaaaactt tcagttgata
    12601 ttcaccttcc gggaccacag tgctgtataa cgttataagc ccgttccccg gattcgggaa
    12661 gatctcaatt tccgaacttt ttaccggttc ttcgaggccg gtgcagtcgt aaaaatgaat
    12721 ggaaacactg tcggtgttaa cgcagttgtt aatatcggtt attgcgacag tgaatccgaa
    12781 tgtgccaagg ccaaggccgg cggtatctgc ctgaatggcc gatgtagttt caccgccggg
    12841 ctgccagaga tacttgatga aattcggtga tgaggcatct atcaggacta cctggttacc
    12901 acagacagat tgatccgtga gctgcggatc ggggagggga tgcacggtga tttcccgtgt
    12961 tgcccaaaca tcgcatccgg ttgttgtgtc ggtaatggta taggtaaggg tgaaagtccc
    13021 cggccctgat atcgccggat cgaattggct ccccgttacg ccggttcctg aaaatgcacc
    13081 tccggccgga gaaccggaga ggttgaacgg tgaagtgcca tcacatacgg aaggaacaga
    13141 atccatcgag acttcggcta gtggcagaac tttaatcaaa cggcttgctg aatcaatgca
    13201 tccgtattca tcagtataaa tataggacag gctgtgtatc ccggcgcccg ccgtcgttgc
    13261 cgagaacagg tcagggcttg ttattccggg cccggtgtaa taaccgccgg caggtgagcc
    13321 ttcggtaaga ctgaaaggaa cggcatttcc gcaaattgag tccagcggcg caaaggaaac
    13381 ttccggcaag gaatgtataa caagagggac actggttgcc ggactggcac aagtgttaac
    13441 tgtctgggta accgtgtaaa gataaacccc tggttgcgtc tgataaggag aataaacagt
    13501 atcggagcca accaaaaagc cattaaagaa ccattggaga tgagtccctt gtgccgttaa
    13561 atccggaacg ggctggccaa aacatacttc agctcccgca gcggcaggag ccgggggaac
    13621 ctctgaaacc gtaatgaagg atgatttgac cagggtgtcg gtcccggccg cattggtcac
    13681 tatcaggctt acatcgtaaa cgccggccgt gttgtaaacg ataccctgag gatttttttc
    13741 gtccgaagtt tcgggtatac ctccggggaa agaccattgc caatcggtag gagggccgga
    13801 agaaagatcc tgaaaactga cgggtaatcc ttgacaggcg gaggcgtcag agacggcaaa
    13861 attgcagaca ggcggcagcg gattgatggc acggatggta accatacgtt tatgaacggg
    13921 agtgccgttg gtcccttttg cggtaatggt cattgtgtaa tcgcccatcg ggactgcgcc
    13981 tgccggaacc actgaaatgg gaagttcacc agggaatgac catagtttat ttccatccgg
    14041 gaaagtaaca ttaaaaagtg aagcggcgga ggcatccaac tcggccgaaa taaatgccgt
    14101 atcggtataa tgtttaacgc cgggaacttt aaaaagaaaa tcagccgtac tactcagcgt
    14161 atcaatggaa ggttccagac gcatggcgaa atcagggaaa taggctacat aactgtcgaa
    14221 tttccccacc ctgaaatcgg tccaggccat cagggaacca tatctgttgg aggccactcc
    14281 gttataatcg ccaaggtaaa caggggtccc gccgccaccg caggtggtac agttgatcct
    14341 gaatttttgc tgagataacg gcacgttagg ggcaaaaatg actccgtcgt cgctgtatga
    14401 cgcgtaaagc aggcaactgt cgcttgtggg gcaatcgcgg gtatcgagcc atgaaatata
    14461 gagccgcccg gtttccttgt cgcaccagat agcaggaaac cagttgtaat tgttttccga
    14521 ttgggtatcg tcgtttacaa ctgtcgccga tgaccaggta gcagcggcat catcggaata
    14581 acggcaaaag atatccggtt ttttcccatt acccgacgga ttgtttgaag catataccag
    14641 gtaaagcctc ccccggtgag ggccggcgga attgtcggcc gcaataaagg gatagggcct
    14701 ggtgcgcatg ttgtgcactg aattccttcc gtttacgttg acgccgatat aatttgagaa
    14761 ttgttgctgt gaacgctggg tgaaggtcaa gcctccgtca acggattgat aaaaagtata
    14821 caacgaggaa aatgaactac ctgaattggt gacgacaaaa acagatcctc cgttgatggc
    14881 gccgttaggg ccgacagcta ccatcattcc gggaagattc tgtgtggaga aatttttagt
    14941 gttctgatac gaggcgccat tgtcggtact tcgcgacagg tttcccccct ggttggcagt
    15001 catggtgttg aatacataat ttgcgaatgg gccgtcggtc tgatcggcgg caatccagtt
    15061 tttatcaacg ccaacgatgg catttacata actgttccat gtggcaccgt tatcagtcga
    15121 aacagcaacc ttagctccgt cgatgcctcc gtacatgttc tgatacaaca ggcggccctg
    15181 tccgtcgtat gtaaccacaa aatcgcccat catgttcgtc cagtcaggat tttttgccgt
    15241 ccagttgtaa ccatcgtttg tataatagcc ttttccgccg gcaagcgatg tggaattcca
    15301 ggtagcataa tactgtaatg ggttaagggg attattggta atgctgcatt cggcaaagtc
    15361 acgtcccagc agaaaattgt catagtcgtt gatagtaacc accgaggacc ttatcaggcc
    15421 ttcacctgaa cggtcatatc cgggtttcat caccatagga tctctcagta tattcctgtc
    15481 gtaagttgcc ggttgggctt ttatcaactg aacagaagaa agaagaaaga tcccggcaat
    15541 aaaaaacgcg tgaaagataa aagctttcat gttttattat tgaattgatt tatacctggt
    15601 tttagtgata aaaatactat tatttctgat agttgtaaaa ggtgaaggaa aaatcagcta
    15661 tttattctaa atttggctcc tttttgaaat aatcattaac cttaaactaa ctaccatgaa
    15721 atccaaatta attcagaaaa caggtatctc aatgttgttg atggggtttg tcgttttatt
    15781 ctcaggatgt cccttttcgg gaagcgtgcc gattgataat ggaacggttg ccattgccga
    15841 ccagttgaac ggtgaatgga ttaaacccac cgacctgacg gaagaaaatc ctaccactta
    15901 catgattcgc cagggagatg acaaatatca tgctgtcgcc gagaaatcag aatattcgac
    15961 ggatgatgcc gaatataata caacggttta caacctgacc ttcagcaatg tttcgggcga
    16021 aaccttcatg agcgctcagg aagcgggcac aactacctac tactattata aatttgcttt
    16081 caataaggaa acaaaagaga tctcgattac ggaagtcagc gactatatca aggagacctt
    16141 taatacttcg gaagaactta aaagcttcat agccaactac aaacagttga gtttcttttt
    16201 taccaatact accgatactt atcagattag gccgtcggat aaataaaacc ggctgatcga
    16261 ttcctgttca acaggactac aggtttcgta tcacgccggc tatgagccgg ttgagctttt
    16321 cttcagccag gcctgccgtc ttcacgatat cgttgatatc ggcgggcagc aggttatcag
    16381 gatcgcattc gtcggtcagg actgaaattg ccgcacaggg cagccccatg tggttggccg
    16441 taatcacttc gggaactgtc gacatgccta caacatcggc gccgatggag cgcaggtaac
    16501 ggtattcggc gcgggtttca aggttagggc ccggaacggc agcatatacg ccggaatgga
    16561 tcctgatatt cagcgcatca gcctgcgaca ggatcagttt attcagatgg gcagcataag
    16621 gacggctcat gtcaggaaaa cgggggccga gtttttcgta gttgattccg gtcagcggat
    16681 tggctggtaa aagattgata tgatcatcca ggagcataag gtcgcctttt ttaaacgaag
    16741 ggttgatggc gccggcagca tttgaaagca gaagatacct gacacccagc gctttcatca
    16801 cgcgtatggg gaaaacgatc tgatgcatgg tatatccttc ataataatga aacctgccct
    16861 gcatggccaa tacctttttc ccgctgaggg ttccgtagat aagccttccc cggtgatatt
    16921 ccactgtgga taacgggaag tggggaatgt cggcatactc aatggatttt tctatggcga
    16981 tctggttcac caggttaccc aacccggtgc ccaatacaat gcctgttccg gcttcggtaa
    17041 tgccttcgga ttttaggaat gaggcagttt cgttaatgtt ttcaatcatt ttaatggaat
    17101 aatttgttgt actttcggga agcgaatgta ggaaattcag ccggatagat gataacgcga
    17161 agggtgcagg atttcgcctt tttaaccatt gcccgccagg gagtattaat ggaataggta
    17221 atcgttgccg gctggattat tatttatagg gcacgactca ggaaatcggg taagagacca
    17281 tggacactga taatgaacaa agcctcgaaa ggctcaggat attgctggag aagaacttct
    17341 cgtggccgtc ggtttatatg tttaagctga tcattcctgc tgataaccgc atctttgcca
    17401 tggtgaaaaa tttatttccc gaggaagcca ggtttttcat ccgaaattca aagtcgggga
    17461 aatatatcat tatcactgtg aaagagctta tgctgaatcc ggaagaagtc attgcccgtt
    17521 accgggaagc gcttgaaata gagggaatta tcatgctttg atccgaaacc tgcaggtttt
    17581 ccagtgcagg aatgaggtat aaagcattgt gaaaggccgg gaataacgaa gccgggaaaa
    17641 ccggcaaatt ggccgttaag tcatgacaga ggacccgcta tcgggcctta aatacaactt
    17701 ccttcagaat gctgtattca tgctcgaaat tggacgtaaa aattcctttt cccagggatc
    17761 gttctatttc ggaacgcgtc cagaaacggc cttcatccac ttcggaagcg ctgatatgaa
    17821 aaggcccttc gtaaagcgtt acaaatgaaa aagatagttc cgattctatt tccgtatccc
    17881 agcgataccg ggctaccggt tgtggtttga aatccttcag cccgatttct tcggaggctt
    17941 cacgctcaag ggcttgttca acagtttcgc cgaagctgat atgaccgcca accgaagtgt
    18001 cccattttcc aggttgtacg agtttgttca ccggacgttt ttgaaggtag atttcacctt
    18061 tgcggttgat cacatgcaga tgaataaccg ggtgcagcaa accgggtttg ccatgaactg
    18121 cagaacgcgg cgcttttccg gtgatttttc cttcctcatc gaccagggga agccattctt
    18181 cgccggcaag ccgtctggcc ttgcgtcgat ttgaaataaa ctgcccgata aagataacgg
    18241 cgaaaaggat gtagaataat ccgccgctga tgaaagccca ggcttccttg ctcataaaaa
    18301 aggcggaata gagcaccagc aacgtgtagg ccgtaaaaaa ccaaaagagc agcctgacca
    18361 tcttttgcat ggcttgttcc tgctcaggtc caaaggtgat atctttaaag taatgttggg
    18421 aataaagcag cagcaggttc ttccgcgaga aggcagaaag tccgagtacg gcacagaaga
    18481 tcaggttgat gaaagcaggt ttcagcttga agaaaatgtc gtttttgagc agcagggaaa
    18541 tgccgcccat tgcaacaaga agcgcggtat cgaaaatgac gaaacggtca agcctttttt
    18601 ctctgatata ggtgtaaacg agttcaacga ttccggtaac gatagccacg ataagcccta
    18661 cttccgttcc ccatacttca tcggcgacga tgaatatgat aatcggcagt aggccgggaa
    18721 gcaatttttt aagaagttcg ggaggcaggg agttcaagga aggaaaaatt gaaaaacctg
    18781 acaggcttcg gaacctgcca ggtttttaga tgtaagggtt ttattttgcg tagctgatag
    18841 cccttgtttc acgaattacc gtgatcttga tttgtccggg ataggtcatt tcttcctgga
    18901 tctttttcga caggtcgtat gctatctggt tcgattcttc atcgctgact ttatcggctc
    18961 caacgatcac gcgtaattcg cgtcctgcct ggatagcata ggttttcacc acgccgggat
    19021 aggcaagggc cagttcttcc agtttgttga ggcgttgtat gtaagcctca actacttcgc
    19081 ggcgcgcacc tggacgcgct ccggaaatgg cgtcgcaaac ctgcacgata ggcgcaatca
    19141 gcgattccat ttctacttca tcatggtgag ccccgatggc attgcaaata tccggctgct
    19201 ctttaaattg ctgggcgatt ttcatcccaa ggattgcatg cggcaattct gcttcatttt
    19261 cggcaacttt gccgatatcg tgcagcaggc cggcgcgttt ggctttcttg acgttcagtc
    19321 ctaattccgc cgccatggtg gcgcacaggt tggctacttc gcgcgagtgc tgcagaaggt
    19381 tctggccgta ggaggaacga tagcgcatct ttccgatcag gcggatcatc tcatggttca
    19441 ggttgtgaat tccaaggtcg atggccgtgc gtttaccggt ttcgatgatc tcctgttcaa
    19501 tctgtttctt ggtttttgca acgacttctt cgatgcgggc cgggtgaata cggccgtcgg
    19561 tcaccagttt gtgaagcgag aggcgtgcaa cctcacggcg tatgggatcg aatcctgaaa
    19621 ggatgatggc gtcgggagaa tcgtcgatga tgatttcgat gccggtaagg gcttcaaggg
    19681 cacggatatt ccggccttcg cggccgatga tgcggccttt tatttcctca ttttcaatat
    19741 tgaaaaccga aacggtattc tcaatggcat gttcagcagc cgtgcgctgg atggtttcaa
    19801 tgacaatttt ctttgcctct gaattggctt ttagtttggc atcttcaaca atttcattga
    19861 tatggaccat ggcttcagcc ctggcttcat ctttaagtat ttcggtgagt tgagcctttg
    19921 cttcgccggc agaaattccc gatatggctt ccagtttttc aatcgtgact ttgtgttcgc
    19981 gatcgatatc ctgttgtttc ttgttgagta tttctttttg ttgttctaag ttaacctgca
    20041 gatcgttgat ctctttctgc tttttctgag tctcttctaa tttttgtgag agttgttgtt
    20101 ccctttgttt gatccggttt tcagcttttg caacattatt gttccgttcg ttgataacct
    20161 tttcatgttc cgatttaagc tggaggaatt tttctttagc ctgaagtatt ttctcctttc
    20221 tgataacttc ggctttttct tcggcgtcgt gcagcaggtg gatgctcttt ttctcgatgg
    20281 ctttgcgcaa caatgttgtc gctaagatca gcgcgattga acctcctgca acaaccccga
    20341 tggcaatcag cattatagtg cccatggcgg tgttgattaa ggtgaaacaa atgccggggc
    20401 aggtagcaga aaaaaagaaa acccgctatt aattcagtgt tttgggaaac cccaaaacga
    20461 aataattagg gtagctctaa atcgtcggac ttctgctgtt atcccttttt caagggtact
    20521 acggagtagc tgcggcctgt cctgatattc acaaagcgaa tccctgcagg aaatgtgttg
    20581 agtttccaaa cggcttgaat taatgcgggt taatctttta tgctttcaaa gaacgtaagc
    20641 gccttagtct tttaacgttt cggtcagtac tgcatctatt ttagccaacg tgtcgtacaa
    20701 tttgtcactg gtgaaagctg cttcagtttc agcggcctgc cctgtgatgg aaaaatgcaa
    20761 tgctaccatt gccaatagat cctgtgtatc gttgtagctg aaattctctg catagtgacc
    20821 aatttgctct tcaacttgtt ttgccgccct gcggatggct tcttcctctt ctctcctgac
    20881 aaaaagccgg taaggccttg aggcgatggt gagtgtgata gctatttcat ccgccatggg
    20941 ttatatcgtt tcaatcgttt aaaagatcaa tgcacctgtc aacttcccgc aataactcgt
    21001 ttatcttttt ctttgcatct gcggtttccc tgcccttcgg tatggcgtga gttattttaa
    21061 gtatttgtat tttttgatcg aggtcgtata tggtttgttt ttgttgttcg tgcttttctt
    21121 cgagttgctg gtatttatgc tgcaattcat ccaaagaccg ctgcagttcc ttcattttat
    21181 cggcaacctg cctgctttta tattcgaggc cggtaaccag cgtacttagg ttctgcattc
    21241 ctcaatctat atgttaatgt cttgcatcag caaaagtaac tattaagaag aagcaatgca
    21301 agtaaatatt tattcctgtt attacgttgc aacataaccg gttgactgtt accttgcctg
    21361 aaatttcatt tgccgtaagg cgcgactttt tactcaggtt gcagttcata cggctgttta
    21421 aataactctt ctctcattgc ggctactctt ttatcccgga ccggcttgct tattttcaat
    21481 gattccggga tataataatc gtccgggacg gtaagaattg tccggaagtc aaaaccgccg
    21541 tcatcgtctc tcatgcaatt cattccgctg aggatgtatc cggtcctttc actgtccatt
    21601 ttaaagctcc cgtcatcacg catgggatta ccggaagaag gttcatcccc caaaaacatt
    21661 cagaatgtga aacttcatta aaataatttg ccaaagcctc ccatttggga aattcgtgat
    21721 caatcaatgt ttagatttgc acagcataag aggaacagat aagtaataac cctgcgatgt
    21781 attaacatct tctggtttgg ttttagcatt atcataaacg gaaaaaaggg agaaaaatgg
    21841 tcctgattaa ttgattgtga agatgattca gcagtattta aaaatgattc tgggcatttt
    21901 tacattggtt ttcggactgc cggaatccaa atctcaggat tttgttccgg tttttccatt
    21961 aaatagtgaa gggtattcct gctttcgtat tccggccatt atcaatctgc cgggaggtga
    22021 actgctggct tttgctgaag gccggaccat gaattgtgct gatttcggga acgtggatgt
    22081 ggtcatgaag cggagcgccg atgccggaaa gacctggtcg ccgctcaagg ttttaatcga
    22141 taacgactca cttcaggcag ggaacattac accggttgtc gaccgttgcg atccccggtt
    22201 tccccgtggc aggatttttc tttactacaa taccggcaat ggccaggagt gggaaattcg
    22261 aaccggaaaa ggcgttcgtg aagtctggta cattacatcg gccgatggcg gaaacaactg
    22321 gagcaagccg gtaaatatca caaagcaggt acactgtccg aatcagccag cctataatcc
    22381 ggaatacaac gatccagccg actggcgcgg ctttgcaaac ggaccgggtc atgccatgca
    22441 gataaaaacc gggaaataca aagggcggat tgttgtcccc gccaaccata ccgaaggaaa
    22501 tccccaaaca gattggactg attgttttgc gcaggctttc tggagcgatg atcatggatc
    22561 gacctatcac cggtcggata aactcggcat tccaggttca aatgaagcta tcgcagcaga
    22621 gcttagcgat ggatcggtta tgatcaatgc ccgcaaccaa aaaggaaatc cccggctcag
    22681 gattgtggct gttagtcacg acggggccgg atcctgggat acagcctggt atgattcgca
    22741 actcattgat ccggtatgtc agggcagcct gctggatacc aggttcaggg gtatgcatat
    22801 tttgctgttt tgcaacccgg catcacagac cgaccggcaa aaactgacca taaaagtaag
    22861 taccgataat ggcaaggagt ggaatccagg cctttgcgtg gttgaagggg agtccgctta
    22921 cagcgacctg gttagaattg accggaaaaa tatcggggtg atctttgaaa aaggcagtga
    22981 cggagggatt tattttctat cagtccccct gaaaatggtt ttaaagggaa aaaaggaatg
    23041 aaccggttcg attccggagt gttatatctc aaaaaattat tctcaacatg ctacgtaaaa
    23101 ttctcacccg caccatcctg ctggtttcat ttgtaagtct ttttaccgat atagcgagcg
    23161 agatgctgta tcccattatg ccggtgtatc tccgttccat tggtttctcg gtattgctga
    23221 tcggtattct tgaaggcatg gctgaagcca ccgccggatt aagcaaaggt tacttcggaa
    23281 acctttcgga taaaatggca aaaagggcac catttgtccg gtgggggtat atgctgagcg
    23341 ccatttccaa acccatgatg gcggccttcg tttatccgtt atggattttc ttcgcccgta
    23401 ccctcgaccg gctggggaag ggagtacgta ccggcgcgcg cgatgccata ttaagcgacg
    23461 aaaccacgcc ggaacataag ggaaaggttt ttggctttca tagggcttta gatacggtag
    23521 gcgctgcaat tggcccgttg ctggccctcg tatacttata ttattatccc cagcagtaca
    23581 aatggctctt catcattgcc tttattcccg ggctgatcgc ggtattgctg actttgctgt
    23641 tgaaagacaa acaggtttct tccaaacaac ggtctcaggt gggctttctt gcttacctgg
    23701 gatactggaa gaaagccggt aaacaatata aattgctggt tataggattg ctgtttttca
    23761 cattattcaa cagttctgat gcttttctgt tgttggcggt taaaaatcag ggcctttccg
    23821 ataccgccat gatcggcgtc tacgtttttt ataatctgat atatgccttg ctgtcatttc
    23881 cggtggggat catggccgac cgtatcgggt tgcgcacaac gctggttaca ggattgttcg
    23941 tttttgccgg agtttatggt cttatgggat ttgctgtatc ggtgcctcag tttattatgc
    24001 tgttcggttt ttatgccctg tatgccgccg ctaccgaagg agtatcaaaa gcattgataa
    24061 caaacatggc cgccagggag gaaacagcta ccgctattgg tttctatacc agttttgcca
    24121 gtattttcac aatgctggca agcagcctgg ccggttttct gtggttcgca ttgggaccgc
    24181 ggcccatgtt tttaatatcc ggtatcggag tggcaatagc tgcaatcttc ctgggtacaa
    24241 acagattttt aagggagaaa actacggttg gcaactagct ggctttgatg atacgcctgc
    24301 gtttttcctg tttcatcctg ttggtatgga tgggcgatta acattgtttt gttaaaaacc
    24361 attcaagccg gttcctgttc aaatggctga ttcattttaa gtttgcatta tgaaaaaaac
    24421 atggatatac ttaatcttct tcctgctgat atgctattta tcagggtatt cacaacaggg
    24481 aattcctgcc ggatactttg catctcctct gaagattaac ctggaggcca caggggcatt
    24541 tgccgagatc agggctaacc attttcattc cggaatcgac ttcaagacac tgaaaaagga
    24601 aggaattccg atcctggcgg tggccgacgg atttgtttca cgcatcaaag tatcgccggt
    24661 tggtttcgga aatgccttgt atatcgacca cccctgcggc tacacctccg tgtacggtca
    24721 catgctaaaa tataacaacc tgatcggcaa ttatgtatat aataaacaat atgaaatgca
    24781 atcctttgcg gcagaccttt ttcctgcccg cgaccaggat acattttgga tcaaaaaagg
    24841 agatacaata ggtttctcag gcaattcggg aacttcttac ggagcgcacc tgcattttga
    24901 aatgcgcacc acgataacgg aacgcatcct gaacccttac cttttcgggt atgacaatac
    24961 cgaccagtat tatccttatt tcgacctgtt caaagtctat ccaatggaca actccagcag
    25021 cgtgggggcg tctgatgaac cggttcaata tggcgtcagg aatcctgaac aagggcttta
    25081 ttaccttgat ggtgttgaca cgctgttcct gtggggaaat tttgcattcg gggttcaggc
    25141 tttcgattac atgtataacc ataaggacag gaacggcttt tacggtttga ggatgctggt
    25201 cgataatgta ctctgtttca gcatgcaatg cgacagcttt tccttcgatg aatcccgaaa
    25261 tatcaatgcc tgtattgatt atcctgcgta ttataaatca ggttaccgta ttgtgaaatc
    25321 aaaccggctt ccgggaaatg ccttgtcatt ctttaagact ccggtcaaca acggagtggt
    25381 ccccttcact gacggaaaag cacattccct ggttttcgag gtatatgata tcatgggaag
    25441 ggtaagcagg cttacggtac ctgtagtgag ccgccagccc ggtaagatgg tatcggtaac
    25501 ggatatagtg gatgccgata ccatttgcag aattaattac gatatcgcaa atcgggtaat
    25561 gtaccataat ttagatgtca cgattcctgc aagcgctctt tacgaaaacc ttgatctgac
    25621 gatcaaagag ctgcccacag caaacggtct gtattcggcc cggtatgcgg tgcataatcc
    25681 tgaaaccccg cttcagaaaa attatacagt ttccattaaa gccgataaag ttccggcctc
    25741 gctggctgat aaggcattga ttgtatatat caatccgggt agcggaaggc gttcctccga
    25801 aggcggaaaa atggaaagag ggtggatttc ggccgaaacc aaccgtttcg gagtattttc
    25861 aattgctgtc gacaaccaga aaccttctgt taaactggtt catcaaaagc catcaaaagc
    25921 aaaatcggtt aaggttattg taaaagacga cctttccgga atagccggat accggggcga
    25981 gattaacggg cagtgggcgc tcgttgaatg ggatcccaag tacagactga tgatctacag
    26041 gtacgatcaa ctgttaaaaa ccggaaaaaa cgtctttaaa cttacggtat gggataaagc
    26101 aggaaatacc gctgttttta ctactccggt aaataagaag taaacataaa gtgcttgcag
    26161 ccgcggaata ctttttatct ttgctgcatg catgaaacca ttctcatcct cgatttcggt
    26221 tcacaatata cacaactgat tgccaggcgt gtgcgcgaac ttaacgttta ttgcgaaata
    26281 catccttata accattttcc ggccataaca ggcgaaatta aaggagtgat cctgtccggc
    26341 agcccgttct cggtaagaga tgaaggagcg cccattcctg acctctccgg tatccggtca
    26401 aaagtgcctt tgctgggtgt ttgttatggg gcccagtatc tggcgatgag cagcggtggg
    26461 gaagttcttg catctaaaat acgtgagtac gggcgggcta atcttgaata tataggcccc
    26521 ggatctgatc tcatgctcgg catgtcgcag aattgccagg tgtggatgag ccacggcgat
    26581 accattcaga aagttcctga taatttccag attattgcag gtacacgcga tgtggctgtt
    26641 gccggatttc aggtgacggg tgaaaagact tatggaatcc agtttcatcc ggaagtatat
    26701 cattctaccg aaggcgctgt cctgctcaga aattttgttg tcgacatttg cggctgttcc
    26761 caggattgga cacccgcctc gtttgtggaa actaccatac atgaactccg ggagaaaatc
    26821 ggcgacgata gagttgtgct cggtttatcg ggtggcgtcg acagcacggt tgccgctgtg
    26881 ttgctccacc gggccatcgg caaacaactt tattgcatct ttgtcgataa cggtcttctg
    26941 cgtaagaatg aattcgagtc ggtactggct tcctatcaac acatggggct taacgtcaaa
    27001 ggcgttgatg ccggcaaaca atttctcgct gccctgaaag gaatcatgga ccctgaatac
    27061 aaacgaaaag ctatcggaag gacgtttatc gacgtgtttg accaggaggc gcatcttgtt
    27121 gaaggcgcaa agtggctggg gcagggcacc atctatcccg atgttatcga gtcggtttca
    27181 gtgaaagggc cttcggctac catcaaaagc catcataatg tgggaggtct gccggcatac
    27241 atgaaactga aaattgtcga acccttacgg agccttttca aggatgaagt ccgcaaggtg
    27301 gggcatacgc tgggtatcga cccggatatt cttcatcgcc atccattccc tgggcccggt
    27361 ttgggcatca ggatactggg cgaagtaacc gcggacaagg tgaagaaact tcaggaagcc
    27421 gatgctttat ttatcgaagg actcaaaaaa aacgaccttt acgataaagt ttggcaggcc
    27481 ggcgctatac tgcttcctgt tcaaagtgtc ggcgttatgg gtgatgaacg tacttatgaa
    27541 aatgttgtcg cattgcgcgc cgtaggctca accgatggca tgactgccga ctggtcgcac
    27601 ctgccttatg aatttcttgc atccatatcc agcgagatca tcaataaggt gaaaggtgtc
    27661 aacagggtgg tttatgatat cagttccaag cctccggcta ccatcgaatg ggaatagcaa
    27721 gcgcaagtgg gattatatat agcagtactt catgcaaaaa aaattcttta tcttcctgtt
    27781 aatcgccggt ttcctgtttc aggccggatt tgcccaggaa gctgccatca aacggtctac
    27841 agtcgttgag caatataagg gcaaacctta ttacctccat tttgtcaaac agggcgaaac
    27901 gttgtttgct attgccaaag cctataatgt ggatgttgag gtcatcaaag ccgagaatcc
    27961 cgatgtggaa aaagaggggc ttaaggtaga aatggtactg cgtatccctt atggtgaaac
    28021 ctccggtcag cctgccgcgg aaaagcaaaa acagatctcc gataaagtaa tggatactgt
    28081 taaatttcag gcctcaaaac agcaattcat cgaacacgag gtgagcaaaa aagagacttt
    28141 gtatggcatt tcaaaacaat acggggttac cgttgacgac ctgataaaag ccaatccgtc
    28201 gatgaccgaa ctgaaaaccg ggatgattct gaaagttccc gttatacagg cggctaaacc
    28261 gacggttgaa gccgagaaaa agaaagaacc ggaacctccg gcaccaggcg tttctggaga
    28321 cggactgata gaggtgaaac aaggccagac gctctatagt attgaaaaac agtataatat
    28381 ttccgaagag gaactgctgc gccttaatcc ggagctgaaa gacgggctga aaacaggcca
    28441 gacgatcagg ctcagggaaa atgccgggat catgcagaaa gggaaaacag tacttcccgg
    28501 acaggagaaa tcagggaaaa cggaaactgt gagtaatgcc gtatctgcta aaccgtttgt
    28561 aaagaacagc ggaagagtat ttgaggtggc tttgttgctt cctctcaggc ttgaaatggc
    28621 cgacagtatc attaagacgc cggtcgaaaa gctcccggaa ctgaataaat ataagacgtt
    28681 cgatttcttt cagttctatg ccggagctat gatagcgcta gatacgctgg aaaaagaagg
    28741 ataccggata aacttccatg tttatgatgc cgattccgag aaagataccc tgaaagtgaa
    28801 aaaggcgctg cgcaggagcg aaatggccgg gatggacctg ataatagggc ctctttatgt
    28861 gaaaagcttc ccgatagcag cgcgttttgc ctcgcaacac cagatcccga ttgtgaaccc
    28921 cctttccagg agaaattcca tcctcgagaa aaatccatgg gtctataaag tttatcccgg
    28981 cgaccaggat attgcaaatg cccttgcttt gagcgttacg aattattacc cgggcgccaa
    29041 tattatttcg gtgtgcaata ccgataagga aaacaaagtg atggcgcttg cttttgccga
    29101 ttcggttaaa aaactgaact cccccggatt gacgctgacg caggtaattt accaaaatgc
    29161 cggtttttca ggcgtaagta aagtcatcga tcccaagcgt atgaatattg tcattctctt
    29221 ctcaacaaac cggtcggtgg ttccggcttt tgtttccaaa ctgaatgcct attcaaaatc
    29281 gggaaatgta gtgctctttg gcatcccggg gtgggaggac atagagatgg aaaccgaatt
    29341 cctgatgaac atggattatc accaggtagt ttcttcattt gttgatttca gcgccgggaa
    29401 tacgaaacaa tttgaaacgg catttcgcga aaagtacggc gctcagccac tgatagagaa
    29461 agatgctttt ctcggttacg atattgtccg ctatttttta ggcgcattgt cagcgtatgg
    29521 caaggatttt aacaaattcc tgccggagta taacctgccg ggattgcaat acaatttcag
    29581 gttcaaacag cctttgccgg ctaacggcta tgaaaatacc gatgcccgta ttcttcattt
    29641 taaggattat cgctgggtgg agaagaaaac cgattaacgg ttatgaaatt ttctgttctg
    29701 atcaaaaatc gttgattgaa tcttcaaggt ttggatacat gttattgttt cgaatcatgc
    29761 taagaaaatc aacaacatac caaagtagag gaaaaaatga ttaaatttat cgcttaaaat
    29821 acaaggaact gggatgaaag aaaaactgac aattgataag ttaatagcag aagccaaagc
    29881 cttttgtata gcagaatcaa atattaaaaa tgaatctctt tttggtgtaa ctgacggaaa
    29941 agctgttggg acgcatattg agcataagtt tcaggaacaa ctgaatgcaa aatatgaaac
    30001 aacaataggt tcgtcagcat acggaattga tttgccgtcg gacgatatat tgacggacat
    30061 taaagtcacc tcaataaaac aacctcaatc atcttgcccc tttaaagatg ctaaacaaaa
    30121 gatttttgga cttgggtaca acttacttgt ttttgtttac gacaaaatag atgaaccaaa
    30181 aaccaaaacc gctatacttg actttgttag ttgctcattt atatcaaaag agcgtacagc
    30241 ggactacacg acaacatatc gtttgtgcga aatggttaag gataaggcta atgcagaaga
    30301 tattgttgcg tatctgaatg ataaaaatat tcctgccgat gaaataacac taaataagtt
    30361 agctaaacaa attctacaat cacctccagc tcaaggatat ttgaccattt caaacgcatt
    30421 acaatggcgg cttcaatacc aaagggtagt ttctcttact gagtcagtag atgggataag
    30481 taaagttatt gataaagtga ccaaataatg agaatatttg aagcaaatat ctcatcacaa
    30541 gtttgtactt tttttaaaga ctttttaatc aatcatttag cctttgaaga agcgaatcaa
    30601 aaaatgtttg ataccttcgg tatcaatact ttctttgata ataatgatga atttcagaag
    30661 ctgaaagaat ctgtttctga taccgctaat agtatattcg aagaaccaga ccgggcggag
    30721 tatggtgatt tccaaacaaa cagggatttg gcatttaaag tgactaaaca attattcgaa
    30781 attgataatt cacctgaaat tgtaattgaa cctacttgtg gaaaaggaaa ttttattgtt
    30841 gcatcgttat cttgttttaa aactataaaa aggttgtttg gaatagaaat atacaaaccc
    30901 tatgtttggg aaacaaagtt taacattatt gatttttgtc tttcgaatcc tcatattaac
    30961 aaacccgaaa ttaatattat ccacgctagt atttttgatt ttgatttcaa taaaatttct
    31021 aaacaatacc cacaagaaag cttattaatt attgggaatc cgccttgggt tacaaattca
    31081 aaattgggca gtttgaattc ttcaaatttg cctcaaaaat caaattttaa aaatcaaagc
    31141 ggtttagatg caatgacagg gaaaggcaat tttgatattg cagaatatat tacattgatg
    31201 ctttttgatg cttttcaatc tcataatggt cacttagctt ttttggttaa aaattcagta
    31261 attaaaaata ttttatttga ccaaaaagaa aaacagtaca aagtaagtaa gatagaaaaa
    31321 tattgcattg atagtaaaaa cgaatttaat gtttctgttg aagcctcatt actggtttgt
    31381 cagctcaatt caaacccaag tattgaatgt actgagtttg atttctataa tagaaataag
    31441 aaattgagct tcggctgggt taaatctaaa tttgtttcaa acattgacga ttataaagcg
    31501 acaaaaaata tagatggcgt ttgccctttc gagtggagac aagggataaa acatgattgt
    31561 gcaaacgtta tggaactgga aatgataaga ggacacttta ttaataaact ttccgaagaa
    31621 attacaatcg aagaagatct agtatatggt tttttaaaaa gttctgatct aaaaaacacg
    31681 gtaattaaaa atacaaggaa atttacaatt gtcactcaaa caagagtagg acaagaaaca
    31741 tcctatattc aacggttata ccctgcgaca tataaatacc tgaaaaaaaa catttcatct
    31801 tttcaagcaa gaaaatcgag tatttataac ggaaaacctt tattctcaat ttttggtatc
    31861 ggggaatatt ctttcaaacc atacaaagtc gcaatttcag gtttgtataa aacatttcat
    31921 ttcacattgg tattaccgca aaatggcaga cctgtaatgc ttgatgatac atgttatttt
    31981 attggatttg ataaaattga gtttgctgtt tatacaatat tactccttaa ttactacaag
    32041 acagaagagt ttttgaaatc aattacgttc tcagatgcta aaagggtttt tactaaagac
    32101 gttttgatgc ggttagattt attaagtata tcaaggtcta ttccccaaaa agaaattgaa
    32161 gaacagatag accatttaaa cgagaagtat tgtttaaaca taaatatctc agaatgggga
    32221 aatttcattc ttgaaatatc accaataaag gaaaatcaat taacattatt tgattgatag
    32281 aaacaatcag gaaaggcata ttttgtgtat aaaacattgg agtttaattg gtaaattcga
    32341 atcacctgct ttgcatcgaa gtttgtgcca atggatagtg ccgaagacta caatccccaa
    32401 tgtttcatac acgcaaatac gctaacgaaa tatcagtcta ttcaacctgt cccatgatca
    32461 ccgtatagtt ttttccgtac tttttggcgc atttcgggca ggtagtgtac cacatgtacc
    32521 atttctttac tgccattcct ttagatttga cgatgctttc atagtccttg caccattttc
    32581 cggtgtcgct gaaaggtcct tcatacacct tggaataaaa ttttccgctc agcgttacat
    32641 tctcggctcc gggaacggtc ttgtccacgg ccaggtaaac atccatgttc cacttcgatg
    32701 tgtggtcgct caggcatagg taatcctgaa ccgcagcgct ttcctgcttc accagtttgt
    32761 cgagccgtcg catggctttg tcgaaattga gcggcatgta aaagaaggtg caaacatgcg
    32821 atttcaggaa tagcttgttg ttccattcaa tcattttatc atcccatggc gccggttcaa
    32881 aaggagggca gcagataggt tcgttttcgg gttgtgttgt catataattt gatcatttaa
    32941 gttgcaaagg cttaacagga ttatcagttc ggcaccgcat caggggctga tgcttatttt
    33001 aagtcaataa aattaaaaaa ttcatcagtt tcggcaaatt atttttaaag actttgtgtt
    33061 tgcaggatca gggttgaccc ttgaaaaact tttccatgaa acggaggcag tcaggcgctt
    33121 atgctttcat ggctttcctg atgccggctt tcaacgacag cgattttatt atgcgggcca
    33181 gtataaaaat caggacaaaa acggaaataa tggtagcacc cgacggcagg ttcatgtagt
    33241 acgaaatcag aagcccggta aacgatccca caaaactgat gatgatggag agtagcataa
    33301 tccgtttcag gtcgctgaca aaaatgttgg cagtggtttg cggaatggtg agataggaaa
    33361 taacgaggat aattcctgcc acacggatgt gcagtacgat ggcgagcgcc accagcgaca
    33421 gcaggatata attgaacaat tcaacggggg cgttgtgtga gcgggcatat tcttcatcat
    33481 aagagatgaa aagtatggtg tggtaaaaaa aaccgaaaaa caagatcgta attaaagtaa
    33541 ggatgagcat cagccataaa tcgggaaccg aaacggtaag gatgcttccg aatagataat
    33601 tcatcaggtt gggggcataa ccgggagtaa gaaaaataaa gatgatgccg agggccatac
    33661 ccagcgacca tacaataccg attgcggaat cttcgcggat ttcggtccgt ctggaagcca
    33721 gtcgtatgct gaacgccgaa agcaggctga agaccaccgc cccgatgata gggttcaagc
    33781 ccaggaaata gccgatcccg atgcccccga acgaagcatg ggtaataccg ccgctgatga
    33841 aaaccatccg gcgggaaaca atatatgaac cgatgatgcc gcatgccacc gacgacatca
    33901 cagccgccag catagcgttc ctgaaaaagc cgtaggaaaa taattctcgg atggtatcaa
    33961 tcatggtcgg gtaagctgtg ttgatgatca tgttcgtgct tctgcaggac agtgtggggt
    34021 atatcgccat gggttatgat ctgcaacgga cagttgtaat cggctaactg ctgttcggtg
    34081 atgatgtttg accggtggta atgcagatat acatttacac aggcaattgt ttttacataa
    34141 taagagatag tccctacgtc gtgcgtaact atcattatgg ccattttctt gttcaattcg
    34201 ataaggcgtt ggtacaggtc gctctcaaaa cggttatcga caaaggtatt gggctcgtca
    34261 agaatcagca gacggggatt tgatatcagg gcacggcaaa tgaatacccg ttgcaactgt
    34321 ccgcccgaaa gttcgcggga agtccggtgg gcaagcccgc cgatccccat ctgtgccagc
    34381 aattgatgag ccctgatatt atcatccttc gaataccggt aaaaccttcc gcgtgccgac
    34441 atgagccccg aaagcacaat gtcaagcacg gtgatcggga actttttatc aacctgtgat
    34501 gcttgtggaa ggtaaccgat aaaaccgccg gatctttcgt ccgaccgggt caccgttcct
    34561 ttaaggggtt tgatcaggcc aagcaatacc ttgatcagcg tggttttccc gccaccgtta
    34621 ggaccgatca cgccaataaa atcgagatcg ttgatctcaa ggttcacatc cctcaggaca
    34681 acttcgtcgt tgtatccggc gcttacatcg gtgagctgga tgagttttgt catggtctgg
    34741 attttgaact gaggttttta gccggaatat cccccggttt ctgatttgcc atgctttggg
    34801 cgataaggtc ggccagatgc atgatgtttg ttgtccagtt atatgccatc gggtcaaatg
    34861 taaccagccg tccgtttatc tgttttgcga tagtttccgc gtgacgggta tcaaattcct
    34921 tggaaacaaa aataacgctt atccttctgg cttctgcaag gtcgatgatt tcgcgcatat
    34981 gcacaggcga agggtccttg ccttcgtgtt cgatgctcaa ctggttcagg ccgtattcgc
    35041 gcgcaaaata agccagggca ggatgataaa tcaggataga tttgccctgg tgaggctcaa
    35101 agagactggt gatataacga tcggccgagt cggcttcatg caggaaggaa tccaggttcc
    35161 tttcatacag cgtctttgaa gaggagtcat accggcaaag cgcttcgcat attgaccggg
    35221 cttgtatcct gacggcatca ggcgacaacc agatatgcgg atcgacagcg caggatgcat
    35281 gcccttcgtg cgaatgccct tcgatcatcg tgataccaga tcccgtattc acaatctcca
    35341 gtcccggatt tacggctttt acccgctcaa cccatgcctt ttcaaaatca agcgccccga
    35401 tggtaaaata aatgcctgct ttggaaacct tttccatatc acggggcgat ggttcgaaag
    35461 tttcatggtt gccgccggga gggatcatca ccccgcactc aagtttaccg cccgagatcc
    35521 tgtcgacaag gtatttctgt ggcagaatgc taacaactac gcttgcctcc tgtctgacag
    35581 tcttatgggg tttgcaggag aacaggagag gaaccaggca tgcaagccat acgaaaggaa
    35641 aaagtgtacg catcaataat tacattttca ggaaacaaac ttacgcagtt ctgtccattt
    35701 attaaagcaa aaaacgattt ttggaataaa gctgaaggct tccacggggc aaggttttct
    35761 tcggatattg acgctgccgg aagagtgcga acttctgatc ctgattctga tccaagggtt
    35821 tccggctatc atgaaaagca gttcaataaa tatccattgt ttttttcgaa ttggcggcta
    35881 tcatcctgtc attgatcacc attcgtgttg attgcgcttt tttcgtgcaa ttcgtggctg
    35941 aatgagtata ggtataaatt ttcttattcc ttaggcatgc tcattccata tttcccatga
    36001 taattctgcc tgccgccgga gcatatcaag tccatttttg accgaagcgc cttgctgaag
    36061 tccgttcctg aggaaaaggg tcatttcggg attataaacc acatcgtata gcagatgcct
    36121 gtccgttaac gacgaatagg gtatggaagg gcatgaatcc gtatcggggt acatccctag
    36181 cggggtggta tttatgatca gcaaataatc gccaagaatt gctttatcca actgtccgta
    36241 agcgagcctg tcggatcctg gattccgtga aactaacctg aaaccgatat tgagttggcc
    36301 caaggcatac tgaacagcct gtgccgctcc gccggtacca aggatcagcg ctctgttgta
    36361 ccgtgcgtca agcagtggta ataaggaatc cctgaaggca ggggcatcgg tattgtatcc
    36421 tttaagctga attccttctt tccggcggct gatccggata cagttaacgg cgccgatttt
    36481 tttcgcggct tcgtccagat catcaaggta tgggataacc ggttttttaa atggaagggt
    36541 gacattcagc ccttcgagtt cagcgcgatc ctcggcaatt ttcctgatct gcgcggcctc
    36601 attaaccgga aacagctcat aacggcagcc tgcgattttt tcgtccgaaa atttccgggt
    36661 gaagtattcc ccggaatacg aatgacccag gggatttccg gtcagcccga aaagtctcat
    36721 ggttagtttg cccgcttttt cgaaagggaa agtgcgatct tgtacaggta atagagcaga
    36781 gcggcgccca ggattacgat cacgaccagg tcgatgatgt ggctgtattt cattacttct
    36841 ccccagtttg atttcagttt gaaaccgaca taagtcagga atgaattcca aagaccggcg
    36901 ccgacaatgg tataaatgga gaatttcaca aggttcattt taccgaaacc ggcagggatg
    36961 ctaatcagat gcctgacaat tggtataaac ctggaaatca atatggtgat ttcgcctttc
    37021 ttgtcaaaga aatgctgtga aacttccagg tgatgcggat tcagaagtaa aaatttcccg
    37081 aaacggtgaa caaatggttt gccgccatag aatccaatcg tatatgaaat caatgaaccg
    37141 gtaatacttc cgagcgtact cgcaatgatg gctcccgtaa aagtgaattg cccgtcgaca
    37201 atcagaaagc cgacaaaggg catgacggct tcgctgggaa ccggaaagac catgctttcg
    37261 agcatcataa gtataaaaac tcccgggtaa gctgttgccc cgattactgc aacagccgtg
    37321 ctggcaatcc attcagttag tcccatgaaa caatgagttg gttagaaatt tttggtataa
    37381 tgtttattca atgagttgat gaagcagggt tttataatcc gttgagttca acataggtgc
    37441 ttattacatc cataatggaa ggatcgaggc gcgccttttg ggaatactcc agcaatgact
    37501 tatgaccttc ataatcgagc ggcagcgtca gaaaaagttc cctcacggct tcttttgtat
    37561 taccggcttc aagcaggtac gcaatcctgc ggtaaaggaa gtcaacgttc gaaggctgga
    37621 ggtcgatgcc ttgttcgatc aactcaagcg ctttgtcgat atcgcttaat tctttgaagt
    37681 acagatcgga aaaatcgagc catatttcag gattcatagc atcaagttcg gtaacatgct
    37741 ggtaagcttc aatggcttcg tccgcaagct tcaggtcgtt gtaacaatcg gcaagaatat
    37801 accagtaatc ggcattctgg ggctccagtt tcaccgcctg ttgcatgaat ggcaaagctt
    37861 cctgcttttt ccccaattca tcggtacaaa tgccgatccc catccaggcg tcagaaagct
    37921 gatcctccag ttctatggat ttccgatagt attcgatggc cgtatcgtat ttcttcaact
    37981 gctcataaca gtcgccgata taataataaa ccatcgcatc aggtttctcg tattcgaggg
    38041 tctgcttata aaccctgatt gcctgacgga agagttccat gctttcgtag gtctgtgcct
    38101 tgatatagta tgccggcata aattccgggt caatggcgat agcaaaatcg aaagcttcga
    38161 tggctttctc aaagagttcc agcgatttat atgccatccc caggttgtac cagcccaccc
    38221 cggaataggg gtttttattg acaacctcat cgaaaaaggc taccgattgt tcattcatcg
    38281 agccaatttc aaaacagtat cctatttcat acaggacgct ttcgttatca ggaaacagtt
    38341 cataggcttt tttcagatag acgagggctt tgtcatacag gttcaggttc tcatattcga
    38401 aggcaatgtt cgtataaatt tcatcaaggc cttcactgac aaatttcaac gcttttcgat
    38461 actcccggat ggattcttca tgtttgtcca tggcgctcag aacgccagct ttggtaatca
    38521 ggatatcggg attgtcgggt tcggaaggct gaatttttgt gagcagcctg agcgcctttt
    38581 caggttcatc attgttcaca agatattggg ctttgagaag ctgaaaggtg ctgtccgaag
    38641 gaaaacgttt taaaccaagc tcaatggctt tcagggcact ctgaaaatcg gacccggaaa
    38701 aataatgata aacaatgtct tcgagttcct gcgaatcgaa aaatgaggag ccgttgcctt
    38761 cagagtcttt ctcaaaccgc tcgagcaatt cccgcatata atcctcatct tcatcgaaat
    38821 tatagtccat tatatatcct gcctttcacg gtgcaaaagt aataaatttc ctgcatgcat
    38881 cagaataggc attgtgtatc agccagatgt ttttattcgc cggattcctt tgcaaataag
    38941 gatttcaagc gcagtgaggc aggcctcggc tcatttatct atatttgcat accgatattc
    39001 aaaacaggaa ttctatgacc cttataaaaa gcatttcagg tattcgcgga acgataggcg
    39061 gcccacaggg caatggtctc acgcctcccg atatcgttcg ttttgttgct gcttacagcg
    39121 tttttataaa acggaataat ccgggaaaac ggatcaggat cattgtcggg cgcgatgcac
    39181 ggacctcagg atttatggtg aaccaactgg ttacaggaac gctgatgggt atgggaattg
    39241 atgtaaccga tatcggtctt tcaactacgc ccaccgttga ggttgccgtg atcgacaaac
    39301 aagcagaagg cggaataata ctgaccgcca gccataaccc ggggcactgg aatgccctga
    39361 aactgttgaa tgataaaggg gaattcctta atgctgccga tggcgcccag gtgcttgaaa
    39421 ttgccgatgc cggtgatttt ggcttcagcg aagtgatggc gctgggaaat tatgataccg
    39481 agagcactac gatagaaaga cacatacaaa aagtgctggc attgcccctg gttgatgtgg
    39541 aagccatccg aaaagcggga ttttcggtgg ttgccgattg cgtgaattct accggtggtc
    39601 tggccatccc tgctttgctg cacgctttgg gagtaaacac ggttcatgaa ttgtactgca
    39661 cccctgacgg gcattttccg cataatcccg aaccgttgcc tgaaaacctg gttgatctgt
    39721 cggaaacggt cgttgataaa aaagcatcgg ttggttttgc ggtagatcct gatgtagacc
    39781 gtcttgctat cgtttgtgaa aacggtgaaa tgttcggcga ggaatataca ctggtttcag
    39841 tcgccgatta tattctgaaa aatacccccg gcaacacggt ttcaaatctt tcctctacaa
    39901 gggcgttgcg tgatgtgacc gaaaaatatg gcggcaacta tttggcatca gcggttggcg
    39961 aagtgaacgt ggttgaaaaa atgaaggaga ccggcgctgt gattggcgga gaaggaaacg
    40021 ggggggtgat ctatcccgaa ttgcattacg gacgcgatgc gctggtcgga attgcccttt
    40081 ttctgacaca tcttgccaaa tcgggaaaga aatgttcgga gttgcgcgac gcctatcctt
    40141 cctattttat ttcaaagaat aaaatcaccc tggaagaagg catggacgtt gatgcaatta
    40201 tccgttccat cgaaaataaa tacgaaaagc agccgaaaat taccgttgac gggctgaaga
    40261 tagaatttga caaggaatgg gtgcatctgc gcaaatcgaa tacagaaccc attatccgca
    40321 tctattccga gagcgattcc ttggccaaag ccgacaccct ggccaggaag attatgaacg
    40381 atatcggcga tattgtgacc ggacgttaat aatctgctgt tgataactat tgaaaacctt
    40441 cgggcgccgg aaattgacaa aacttcctat tggcttatct ttaccgcttt caacagtaaa
    40501 taagcgtatt aaccagggaa gaattccggt atggatttcc atgatatccc gggaatctgc
    40561 cctggacaaa atttcaggat catggcgtcg gctaaaaaga aaaaattccg gaaggcaaaa
    40621 ttctataagg ttgaattcaa aattccggaa gcattacata aacaggtgaa gaaatactgc
    40681 aagaaaaacg gcattactcc caataagttt caccgccggg ctatccgtga atacattgaa
    40741 cttcatgcta agtatcccga acatcgcgaa gatgccgtag gcccgaacca aatgtcaatt
    40801 tttgacatca ttgagaataa caaggaagaa tagaaaactg ttgcgcaggg gaattccttc
    40861 cggtaatttt catcgcgcct attaggggct gtttcccgtt ttaatttccc tgaataaaac
    40921 aagaggctga cttaatagcg cagcctctta ttttattatg atcccgctga gatccgtttt
    40981 tctccgtctc tcagatcagg atatttcagg tgaacgaatt tcagtgctca cgggacgaaa
    41041 attaaaaatt gattttaatg tttgccagga attcccttcc gggaccaggc tgatacgaat
    41101 tgccgtcagg atcaggttcc gtgaaagcca tgtatttttc tccggtgaaa ttgcgggcat
    41161 acaaactaca ttccccgttt aatttccaga aattccacag gtaagcaata cgggcgtggt
    41221 acaggttgaa tccgtcctgc cagttctggt aaatggcggg atccatttcc ccgctgtatg
    41281 ccttggcatc ggtatagatg gcccattttg actggtattc ggtggccaga ctgaactgca
    41341 ggtgatggtt aagttcatag gttacttcgg catataactg gtgttttggc gaattgggca
    41401 accactgtcc cgaagcagga gggagcgtga gaacataagc tgtatcggtg tatacggggt
    41461 caacgtcggc tgatgtatac ttgtaatcgg cataggtata ggagagttgc agtgtcaggt
    41521 ttttcaggat ggcgtatgac aggtaggttt cgataccgat acggtcactg ttaccggcat
    41581 taccataaaa tacctcctgg ttgccgcgac ctgattgttt gaaccggaag aaatcattct
    41641 cggttttcat tgagaagaaa gtagcttcat aattcagttt ttctccgagg aagccgcgga
    41701 taccggcttc aatacaattg gaggttgccg ggacgaggta ggtgttgaat cctgaataac
    41761 cgaccgggtt attggccagt tcttccgttg agggcggcat aaatccctgg ctgaagttgg
    41821 caaaaaggtt tagctcgtca gtgaaaccgt agctggcccc gatcctaaag gatgttctgg
    41881 agaagttttt ggtggtttta gccgtatcag gtgcatacat cttattggtc agctcattgt
    41941 tcatgtcgtc gtaacggaag tttgctatca ggctaaactt gccgaattca attttatcaa
    42001 ggacaaaaat cccgctgctg cgctgataaa taatctgatt tgccagcagg ctgtcgtttt
    42061 cgaggtttgt ttcatccgtg ctttctaccc tgtcgggatc gctcatgctt tgtaatttat
    42121 acatgtcgat atgctgccat ttaaagtcgg tcccgacgct gaacgtgttc tttaaattgt
    42181 ttacggccat atgcagattg tattgggcgc ttagtccggg attggtaatg gaacggtatt
    42241 cggcacaacg gttgctggtt tctttgtatc gccaggacct taggaatgag gtagcttcaa
    42301 tttcctgctt ttcgccgagt ttataggttg catggatgcc ggcagtggtg cggttggttt
    42361 tctggtattc gttaaacggg catgcgtctg gattagcctg cctcaggtca ttgaattgtc
    42421 ccaggttcag cccttcaggg ttctgctgga agtaatcggt gtgggagatg atctgcgtta
    42481 ccgacaattt ctttgaagga tggaaagtta ctttttcaga aagcttgttg ctccagaacg
    42541 cctggtgatc gcggtaaccg tcgccgccgg tacgtgaata gctgatgcgg taatcaaggg
    42601 attgcttact tccgttcagt tgaatgagcg atttggcgaa gccgttagag cccagggatt
    42661 ggcttacttc tccgccgacg gggttgggcc cgccgttctg cgttgtgatg ttgatgaccc
    42721 cggcagcgct gccgccaccg tataagccgg ctgcaggacc tctcaggact tctattttct
    42781 gtactgttga ccagtctaca tcatataaat cgggggtgaa gcctgaaggg tcatttacag
    42841 gtatgccgtc gatgagtacg cctatgccgc gaagtccgcg ttcggtaaga atgccctgtc
    42901 cgcgaatcga cagatgaact cgttcgccgt catgctggtt gtcgatgcgc acaccgggaa
    42961 cgagtctcag ggcttcctcg gcgccgatgc ccttcggcat gatcgaaagc atccggggag
    43021 taaccagcga catagcgccg ggatttcttt tcagggtaac cggcaagcgg tttgccgtga
    43081 caattacttc ttttaattgt aaaactttgg ttgaatcggt agttctttgt tgaccgaaaa
    43141 ttgcaccgga cataaaaatc aacagtgcaa cgaaaaatat ttcttttctc attttaactc
    43201 ttgtttggga taaattccat gttccgtcag tccttatcgt ttcaagaaaa acgacggaag
    43261 cgggcgacgg aagtcattgt gattaatcag tctttgagga tcggcggacg aaaaagtcat
    43321 acaaaccggc atggggtcat caccagtgta taagtccatc cagtcgccat cctgaagcgg
    43381 tttggcagaa aaatctgtaa cagccgtggc cttcaccgga cttcagcata aaacagccgg
    43441 tgaaatcata gtcagtcttg tctctgcatc gctatcagga aattttgggc gggggagagg
    43501 gcgttattaa aacctgtgat atgtaatgaa agcctaaacc tgtataaata aagtcggcaa
    43561 aagggaaggc aaaagccagg acaggccgtt gtggcaggta tttttgatta tactgtgttt
    43621 tccttctttt ttccgtttct tttttcgatg tatgactttt atgaaagccg gcgatcaact
    43681 gtttttcctt ggtatcgttg taacaataat aatatttatg ctggtctctg gttgccgagg
    43741 aaataacatc atacatctgt cctttatagg caaactccct gcctggcctt atccacatga
    43801 ttcctttctg aaaatcaaga gggaaaacga gcgtgacacg ttctctttcg tccttcgctt
    43861 tcctgatctc accggcgact tcccagcgga tgcgggtctg aaatatccgg aacagatagt
    43921 atccgccacc tgcattaaac aggaagaaaa ctaccaataa aatggatatg ctctttttca
    43981 ttctccggtt gaaatacctt tcgaaacctg tcagttaaac gagattgctg ctgacaggca
    44041 aaaaggttta aacggcgcac caaatataaa aacatcgtgc ggtgtttatg ctgttattgc
    44101 cggaaaaacc tgatgaaaat aaaggatatt ctgaagagga ttccattcgg cctgaaaagt
    44161 cacgaaaagc aggatgacat aataagtaca cctgatgcag gatgccgttg ggcaacagta
    44221 acggatttca tcagggaatc ctgatgtttc agggcctgct tcccgccttc gataacgcat
    44281 cctttaccga ggccttgaaa atattgaatg accacgttgc accggaaata gcgtcaatct
    44341 tgttaacatc ctgatatttt aacaggctgt cggggtaagc ccggacgcct ttccaatcgt
    44401 tcctgcattg ctgaatatat tcaggattgc cggcaaagtg tttctcgtat ttctgattga
    44461 aatattcgtg tttgaccgaa tcgcggatga agaaatccac atggatgatc tttccgtttt
    44521 taacgtcgat cctggtaaaa ccataatagg gttcattcgt atacgaagcc ctggatacgc
    44581 ctcgataatg gccgtccttg tatgttatgc cgatggtgaa ggcagtcagg gctaaaaccg
    44641 ataaaacaac agtaatcttt ttaatcatag gtcgtgggtt gtggcgatca atacgcgctt
    44701 ttaataatta tagatacgcg ggggaacaaa taccctgtgc aaaacaggaa atttttcaat
    44761 acaggaagga actttccccg ataaaccagg gaggacgctg tatcatcgat ccggtaaaat
    44821 tacagatcca ggtataagct tcctgcaacg gaatagaata acaagttgcg ggggatgagg
    44881 aaaaatttct gacttggacg aaagtcaacc caactttgaa cccctgcggc aattctgatc
    44941 ataaaattca gcatgtattt atttcaagga taggtttgtc tttaatgtgg accgtaatgt
    45001 gaatatatat cgactataca gctttttcat tatagacgga ttaataatct acattaaaat
    45061 gcctgaataa tcgccgtgaa ttacgcatta attttttaat tgtgcaaatg tgttttaccg
    45121 ccggtaacgt cttgcccgaa acttccagaa taaccgacaa ttttatctcc ggctgaaagt
    45181 gatataatca gcgcccgcag gggactgttt tgtctggcaa acaaccggcg ggcgcaggta
    45241 gatagcgtat aaatactgct aaatcatggc ggaatgaaga atggtttaga atcccagttt
    45301 cttttttatc tccttcggaa cagcatcttt atgcaccatg atggcgatgg tgttcagcct
    45361 gacataagct tccgacatgt tcagataacc gcccgattca ttactgtcgg cagcccatga
    45421 gttttttgtg atataatata cggtactgtc ctgatcgcgg gtgatcccgg taatatgcat
    45481 caggtggtcg tcggtggctg agaaattatc gaaggcagcc tgtctcattt cctgggtcac
    45541 ggctttctgg ggaacaggtt tgtcgaatga ataaaattgt ttctgacgtt ctttttccgg
    45601 aatgttttcc cacttactcc gttcggtatt gctggccgat tcgagtttaa catccggcac
    45661 gatggcgact ccgttcttgt gtgagaaccc tttgtcgctc acgtcgccat cccacacaac
    45721 cgtgtaaccg tttttgaggg catgattcag ggtggcgatc aggtcgtcaa tgggtacatt
    45781 gtaataagcg cccgatgacc agttatccgg aatttcgagg ataaaaggct gataaaaagg
    45841 atgatgggta taggacgtca gttcgatgta atctgccgga ttaatttttt tggctccttt
    45901 aaccggagaa ccggttgcag gcaaaggccc gagatagaca tcaagaacgg cgctgatggc
    45961 attaaaccag tcaggactga gtttgccgtt cgggtttttt accagagcat ccagcatgcc
    46021 tttcagaacg gcttccattt cggaatgcat gggtttggtc tgtccgttgg caagacccgg
    46081 ataaccggct tctgtcataa gcccggatgc agccacagta ttcattacat catgagcctg
    46141 tccaccctga ctaaaatttg ttgttccgtg acgtctaacg aaatcgcggg ctttttgttc
    46201 atagcacttt cttacaaaaa acatttccga taaatcaagg gtgtccccgg taatacgcat
    46261 gagttctgac tcaatgaatg aagtggcggc gtaactccag caggtgcccg aagcatactg
    46321 gtcttttacc ggggtagttt tcagccggac ggcttcggtg aaaatatact tttcgggctg
    46381 gaccgtgtcc ttttcctgtg cataaacgca aagggcgttc gtgaaaaacg aagctacaag
    46441 cagaggagtg atgacagtga ataatgattt cataggatgg taaggatgta atcaattccc
    46501 aaaattactg atttgtctga aatcggaggc aagtaaaaaa aggaagaatg ccatgtcgga
    46561 atttccagga agagagccgg agatgttgct ttcagccgga gggtatatgc aaaacctgcc
    46621 ggcttgtttc cgtagtgaat gatgaaacct gtgatccggg ggctttaacc ggggagccgt
    46681 agtttccggg caggcgatca acctctcatc gctgctttaa ttatatcttt ttcctaaact
    46741 gaaactgata taagcggtaa cgaaataatg gtaagggtct ttgaacgcca tgatgggtat
    46801 ggctttcggt aaaaaatcga cgataatgcc tgtttcaagc gctttgatcc gttggttgta
    46861 ttctccgaat tcgaaactga atccggattt ggcatagagg cccgggtaca gttttatatc
    46921 gctgaagcct tcaagaaaag gcccccgccc ggcgatatcg caatcggcat tgttataata
    46981 tccccggtat ggaaaatgct tttcaggatc atatttttca agctgcaggt cataataggt
    47041 aaacatgtcc tgctgggtat aataggcgat ataaaggtaa acgggttttg ccagggaaag
    47101 ggtagggccg ccgtaacaga agaatcttac ttcaacgccg ccccagtatg gtttgcggtt
    47161 cagcaatttt tgctgcccga agccggcgcg caggatgtaa acattgtttt cttttccgta
    47221 aacatattgc cgcgcattgg ggatgtactc gttgtatatt ttcgtttcct ttggcgattt
    47281 catttcaaga aggtcgaatt cgagcatccg ctttttgaaa taagttttgt tttgccctaa
    47341 acgaaatccc aaaccgtagc ccagcgtgtg caatgagagc gatgcagcca tctctttccg
    47401 gtacagcaat ccttcggcaa tggtgtcata atccgtcata tcctgtgccg gagacagcgc
    47461 aggagataaa agcaggaaga tcaggatgac aggatactga attttcataa tgaaggctta
    47521 atacaggaac aaaccggcca attctgccca tgaagtatgc tttatgggca acagttgtgt
    47581 gtttaagcca tgcggttatc gcgattcaac ccggtagcgg ctggcttcac catacgcagc
    47641 gcattgttcc gaagtgctgc aggaggaaag caatatgctg agtattacaa cagtggcaag
    47701 aagggctgca attttcttca tgttattcgt ttgagtaaca gttctggtta tattatactg
    47761 atgcaaaaat atctactttt aatgatataa caaaaaaata ccattattat tgtatcctaa
    47821 aaaccgacta attttcatga caagaattaa tccgcgaatt gaggaaggat ggcgggatgc
    47881 cttatacgat gagtttcaga aagactactt tattgcgtta aaacagttcc tgattgaaga
    47941 aaaaagcatg tacagggttt atcctccggg ggaccgtatt tttgcagctt tcgattatac
    48001 gccctttgaa aaggttaaag tggtgctgct cgggcaggat ccctatcatg gtgacggaca
    48061 ggcacatggg ttgtgttttt cagtgcccga tggcgtacgt cagccgccct cgctggttaa
    48121 tatattccag gaattaaaca atgatctcgg gatagcgcct ccaaatcatg gtaacctgga
    48181 gaaatgggca cggcaaggcg tgttgctcct taatgcaact ataacagtgc gggccaatat
    48241 ggccggatcg catcagaata aaggctggga aatatttacc gatactgtca tcaaagccct
    48301 ttcctcgcga aacgacaggc tggttttcct tttatggggg aattacgccc aggctaagga
    48361 agccttgatt gacacgacaa aacatgctgt gctgaaagcc gcccatccat cgcctttttc
    48421 ggcataccgc ggatttttcg gttgcaggca cttttcgaaa accaatgaac tgcttgaaaa
    48481 ctttgggaaa actcctatcg actggaattt gaatgaactt tcgtaaattt aacgtatgaa
    48541 agtttcagca tggttcgaaa atgcggctgg ctcagcggtt aaatgtacgt tgtgccctca
    48601 ccgttgtgtt attcaggaag gtaaatcggg aatttgccat gtgcggatca acaagggagg
    48661 ttcactttat tccgaaaatt tcgggatgct ttcagccata cacctcgacc cgatcgaaaa
    48721 aaagccatta taccattact atccgggttc acagatatta tcgattggtt cggtagggtg
    48781 caacctgggt tgcctgtttt gtcagaattg cgagatatcc caggtgcctt cggtacatgc
    48841 ggtgatgggg ctgaaaaagt acgaacccat acaagtgaca gatctggcgc ttcagtataa
    48901 ggacaatatc ggtattgcct atacctataa tgaacctgca gtctggtatg agtttatgct
    48961 ggaaacagcc atgctggcag ccgctaaagg gcttaaaaac gtgatggtca caaacgggtt
    49021 tatcaacagg gaacctctta ccggattgct ggatgtgatg gatgctttca gcgttgatat
    49081 gaaaggtttt acaaatgatt tttaccggaa aatcacttcg tcgtccctgc aacccgtcaa
    49141 agaagccatt cagatgatct catcatcggg acgtcatctg gaactggtta acctggtaat
    49201 ccccggactg aatgacgacc gatccgattt cagggaaatg gtgaaatgga tcagcggaga
    49261 attgggtaaa acaacggtgc tgcacctgtc gcggtatttc ccaatgcaca agctctccat
    49321 tgaggcaacg ccgccatcgt tgctcgatga attgcatgaa atagcttcgc agtacctcga
    49381 ctttgtttat gtgggaaatg tatcctcaga attcagcaat acctattgcc cggcctgttc
    49441 ttccctgact gtgctccgaa aagggtacag catatatttg acaggacttg atgataaagg
    49501 acggtgtgcc aggtgtaata atcaaataat caagcatata aaacagggaa tggcttaggt
    49561 tttggatggt ttcgcaggcg ctttttatgt taataaatgt aaaaatgcac gcatggggtt
    49621 ttgattttaa aattgaataa aatacttttg attcgttctt aaagaaaata ctactgttat
    49681 aattattgaa ttgccgatgg ctataaacaa aaccaccctc atcagaagca ttgccattct
    49741 tacgctggca tgcatattcc tgcagcgagg tgggtatgta tcagctcagg aactttctga
    49801 agatcctgca ggcggagacg acgactctac cataatggcg cctatgatta cgcctttttc
    49861 gtcttccgta ttacctgagg atcttgatta ctacccgtta aatgaatggt acccgtcctg
    49921 ggataatcaa tatgtccggt tataccataa catatactct tacaaaggcg attccatata
    49981 cctggaactt gagcctgaag aatcaggctg ttttacccct cctgtaaaag gacagttggt
    50041 gtcgcctttc ggataccggg gcagaagaat acatgcggga gtcgatttaa tgcttaatta
    50101 ccgcgatacg gtagtcgccg ctttcccggg cgttgtccgt atggcccggt atttctcggg
    50161 gtatggaaat tgtgtcgtga tcaggcacta taacggcctt gaaacattat acggacattt
    50221 gtccaaaatc tctgtatcgg ttaaccaaaa agttaattcg ggagaattaa tcgggcttgg
    50281 cggcagtaca ggtcgtgcta cctgcaacca tcttcatttc gaaacccgtt ttatggggga
    50341 tgcctttaac cctaagcaac tcatcgattt tgatcatcac agcctaatcc ggaatgattt
    50401 gtggatcacg ccatcggttt ttggccgctc gcgcgactat ctcccgtctg atgaaccgct
    50461 ggcaaagcag gaacctgtcg cagccacctc gcacaaagca ataaaaacta aggcaaaata
    50521 tcataaaatt aagtcgggcg atacgcttta ttctattgcc aaccgcaatg gaacaacagt
    50581 caaaaaactc tgttccctga accacatgaa acaatcaaaa gtactgaagc cgggctcacg
    50641 gatcagggtt aaataaatcc tttatcggtc atcgcaacgg accatcaatc cctgacggca
    50701 ccatatcctt tatgccggtc gttcagccag gcaattgttc tcatacgttt tttcttccta
    50761 tttttatccc cccgccggga ttattcagcc gccggaaata aatcatgcga ccaacccgaa
    50821 aaattcttct ttttttactc cttatcatta ttattcaggc ctgcaaaccg gtaaatgaaa
    50881 tagctgtccg taatttatcc tggaaatacc aggggatgag cgcatcgccg gatatccggt
    50941 gtaccctggt taacgtcaat gattccgtca gcagggtttt catcgccatc ccgccccaat
    51001 tgcctgataa taggtttgat acaagggagg acagtgccaa ggccggatat cgtattaaat
    51061 atgtattgta taattcctgg ctttcggctg ctgtagctga cagcggaacg gttttttacg
    51121 agtttacgcg gagcagagcg gatgataagc cactaaaagc agaattcaac cttcgggcgc
    51181 cggttggaaa tgattatttt ctctacctga aggttctcga tatacagcat gaaaaagagt
    51241 cggaagcgct gaaaggactt ctgaaaagga atgaaggaac agccgactgg ttcatggtaa
    51301 acgatgccgg cggtgaatta atgaaccagc ggtacctgag caacaacagt cctgtcggcg
    51361 ttcgctgcta taatccggct caggaaaaat tttatgtgaa ggttttccgc catgttttcc
    51421 agcctgcttt gccgcctttt gtcatggaaa tgcgtgaacc gtatgccgaa aacgctgaca
    51481 gcatatttac aatacttccg tccctggaaa acaccgcaag cctttcttta tacgaccagg
    51541 gaatatatca tatccagaat gacagttctc aaactgacgg actgtcgctg gttcgcttct
    51601 acgacggtta tccggctgtt acatcgcctc tgcggatgat tgaagctgtc aggtatatca
    51661 ctacctcaaa ggaatatgac catttgatgc aaagttcaga tactaaagca gccattgata
    51721 gtttctggat tgcaaccggc ggtaacctgt cgcgggggct ggaattaatc agaaagtatt
    51781 atggaagggt agaacaggcc aatcgattgt ttccgtcctg ttgcgaaggg tggaagactg
    51841 acatgggcat ggtttatatc atttttgggc cgccaaatgt cgtttatttt aacgggaatc
    51901 aggaggaatg gatttatggc gaagcccgga actatcattc tgttcatttt gttttttcga
    51961 aagtcgtgaa tcctttttct gatgaagatt atcggttgca gcggcagccg aattacaagg
    52021 aattctggta tatggcggtt cagcaatgga gaaggtaggg gcaggattac ggagaatgga
    52081 aaacggaaaa ctttacctgc cagcggaggg ttgtgagtga gcgggcaggg gagcaaggag
    52141 gacggagcta atagctagag gctaacagct aaaagccaaa agaatgaacg gagaacttta
    52201 cctgccagag aaggatcgtg agtgagcggg caggggagca aggagcaagg agctgggagc
    52261 agggagcaag gagaacggta tgatagcctc ctgactttaa agctctaaac atctggtaac
    52321 aacaaatgac accaataaaa tggcttatta tcaaaataat ccctcgcgga tttcacaacc
    52381 tgaaaatatg atctatggcc tgaggcctgt gatcgaagcg ctgaaagccg gcaaagaatt
    52441 tgaccgcatc ctgttgcagc gggatgcacg aaacgaacag gtgaaagagc ttattaaact
    52501 tgcccgtgaa tcaggcgtac tgatgcagca ggtgcctgct gaaaagcttg acaggcttac
    52561 acgcagcaat caccaggggg ttgtgggatt tatggcggtg attagttacc aggctattga
    52621 agatgtattg atggatgttt tcgaaaaggg gaaaacgcct ttaattgtcg tgctcgaccg
    52681 tgttaccgat gttcgcaaca tgggcgccat cgccaggtcg gccgaatgcg ccggcgtgga
    52741 tgccctcgtc attccgtcaa ggggcggagc ccagataaat gccgatgccg tcaaaacgtc
    52801 ggccggtgcg ttaaatacga taccggtcca tcggagtgat aacctgaaag atacaatgca
    52861 atatctgaaa cagtcaggcc tgacgcttgt cgctgcttct gaacatgcca gggataatta
    52921 ctatgatgcc gaccttacgg ttcccctagc tattatcatg ggttcggaag aggatggcgt
    52981 ttctcccgaa tacctgaagt tggccgacgc taaagtgaaa attccccttt acggaagcat
    53041 cagttcgctt aatgtctcgg tggctgccgg gattcttctg tttgaggctg tgaagcaacg
    53101 gaataaaatc tgatggccgc cgtatatttg taaaccggca ttgatgataa agtatgctgc
    53161 cggcaatatc cgggcattct gctgatagat gatctctgct atttacgggg aacaaactcc
    53221 cggagaaaga tataaggcca tagctccata tctccggtta aaccggactg cgttaactca
    53281 cgattattat ctttgcagac tgtgagatcg cgaagggcgc tccttcagtt tcacgtggtg
    53341 acaataacta attccggaag ctgctgatga tgcttggaaa acggtttcaa caaattaaac
    53401 ctgtaaatgc gtggttgctg ctgattatta tcataggagc gattttacgt ttttacgatt
    53461 attccggatt ttcattttca aatgacgaat taagcgcgat caaccggttg cgattcagca
    53521 ctttccggga tcttgtggaa aaaggttttt atgtagacgg tcatcccggg ggcattcagg
    53581 tgatgttatg gtactgggta aaaattttcg gcgtttccga agcatcattg agattcccgt
    53641 tcgtaatctt cgggatttta acggtttggt tctcttatct tacgtccagg caattctttg
    53701 gcgccgttac cggattattt gtttccgcgg ctgttgcttt cctggaattc ccgcttgtat
    53761 acagccagat agctcgtcct tacggatcgg gcattttctt ctgcatgctc atggtttggt
    53821 tttggtaccg ggttgttaat ggatttcagg aaaagacgga gataaagaag aaacgactgt
    53881 taaaccttgc cgggtatacc atctcgacag ccttgtgcat gtataaccat tatttcagtt
    53941 tcctgcttgc cggagttgtc ggtattaccg ggattttcct tataaaacgt gaaaggctga
    54001 ccggatacct ggcggcaggc ctgatagcat gcctgctttt tttgccgcat gtccctataa
    54061 cgttgaatca ccttacatat aaaggggtag ggctgtggct gggaaaacct tcgcctcagt
    54121 ggatatttgg ccatatcttg ttcattttca atgaacaatt atggttcctg atcctcacag
    54181 gaatggttgc agctaccctt gccctgatca acagacaaag aagcgggcta tacaaatacc
    54241 agacggtttc atttctgttt tttattttgc cgatggtcat ggggtacctt tatagcgttg
    54301 ccgttaatcc ggttttgcag catccggttc ttctcttttc tttccccttt ctgctattcc
    54361 tgatattttc ctgtgccggc gataacctga aacccatcca ttccctgtta ctggcatttt
    54421 ttctgacggc aggtatatat tccactgtcg gatgcggcca ttattaccgg aaacagcatt
    54481 tcggcgagtt taaggatgta gctgcccgga ttgcagcaac aggcaaccgt ttcgggaaaa
    54541 acaatgtaac caatgtggta agcacaaatt gtccttacta catctcatat tattatgcca
    54601 aacagggtga cagcgcccgg ttatcgatga cggaagtaac cgccgacagc ctggaatcat
    54661 tagggcgcct ggtaagaaat tgcggaactc cgtacttcct gtatgcgtgg acaaagcctt
    54721 ccccgcctga ggcagatgat atcattatgg cttcgttccc atatatcatt gatgaaaagg
    54781 attatggcgg attatcttcc ctgaaggtgt tttccgtgaa taagccggag aaaggatata
    54841 ccgaaagccg ggtgatattt ttcaggcagg ggctgcctga ggataaaatg cgaaagtctc
    54901 cggacaggaa ttttcacaat tcgcttccgt caggcggtgc gggtttagcc gttattgatt
    54961 ccacagagga atatgggccg gctcttacgg ttccggcttc ggaaatcagg gcgacagggc
    55021 aggaaacagt ccgggcggaa gcgtcggtat atatgtttaa gacgccgacc caggcggttt
    55081 ttgttacttc gcttgaggtt ccggggaaag aacccctcat ctggaaggga gcattggtgc
    55141 ggaattatgc ggatgcggga aaatggcaga aagtagtaca gacattcctg ctgcccgaaa
    55201 aaattgataa gtcttcagta ctaaaaatct atatctggaa caaaggaaaa gaaagtcttc
    55261 tggtaagaga gatgaatctt tccgtggttg cgaatgagaa tgcgtcagat gactaacaga
    55321 atgaattaca ggatgaaaaa aatagtgatg aatcgaccgg cttttctttt ttgcctgctg
    55381 gctgtggtgg caatgggatg tgccaaaaca aaaaccgatt attatcccaa cggatcggta
    55441 atgtcggtgc ggcatttttc agggcgaaaa ttgcagggaa tttctgtatg gttctacgaa
    55501 aatggagcaa aacagcagga agtttcttat cgccatgata aacccgaagg cctgacaaca
    55561 aggtggtttg ctaacggaaa caaacaaagt gaagaaatgt accgggaagg aaagaaaaac
    55621 ggcaaatcgg tcatgtggga tgaaaacggg aacaaggtag aggagcggaa ttaccgtaac
    55681 gataccttaa acgggcaata ctcgttctgg tactctacag gtatggtcaa ggttaccggg
    55741 aattttagca aaggactgta tgacggcaaa tgggaatact tcagtgaaac aggcgtaaag
    55801 gtgggagagg ggaattacga gaaaggaagc ggaattctca cgggatt
//
LOCUS       DAOPYV010000010        54987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_443_length_54987_cov_11.792908, whole genome shotgun sequence.
ACCESSION   DAOPYV010000010 DAOPYV010000000
VERSION     DAOPYV010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 54987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54987
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_443_length_54987_cov_11.792908"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(344..1702)
                     /locus_tag="PKI35_02225"
     CDS             complement(344..1702)
                     /locus_tag="PKI35_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNX43224.1"
                     /translation="MRKQGSASGLGVEKVGKLLLQYSVPAIIATSASSLYNIIDRIFI
                     GQGVGPLAISGLALTFPLMNITAAFGAMVGVGASAMVSIRLGQHDRQGATRILGNAVM
                     LNLILGIAVGLVVFVFLEPVLYALGASPDTLPYARQFMQVILLGNVFTHLYLGLNNVI
                     RASGYPGKAMTVTLITVGINLCLAPLFIFVFKWGIRGAALATVTAQITGTAVSFTHFT
                     KPSSFVRFLPGYFKMKKKIIRDIISIGMSNFLMLICASVVTSIVNLSLRKHGGDFAIG
                     AFGIINSIGSLVVMVVLGFTQGMQPVVGYNFGARRTPRVIRAYKLTVIAGIAVTTFGF
                     LLAEIFPRQISSAFTTSDELIGLASLGMRLNLIMFPVVGFQIVTSNFFQSIGKARISI
                     FLSLTRQVLFLIPALLVMPHFFGLNGVWLSAPVADFSSSLLTLLVLRWQVGKLKTTPP
                     AA"
     gene            2013..4604
                     /gene="clpB"
                     /locus_tag="PKI35_02230"
     CDS             2013..4604
                     /gene="clpB"
                     /locus_tag="PKI35_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNX43225.1"
                     /translation="MNFEQFTIKSQEAIRQAQEIAQQMQHQSIEGIHLLKALMEGDDS
                     VTPFLLGKTGVNTGTLNREMDRLLQSLPKVSGGEQYFSNGLSGVFRKAVALTRDFKDE
                     FVSIELLLYALLISGDAASSALKSAGLTEKGLKAAIAELRKDTNVNSVTAEDNYNSLG
                     KYARNLNQLARAGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGVGKTAIAEGLA
                     HRIINGDVPENLKTKQLFSLDMGALIAGAKYKGEFEERLKSVVKEVISSNGEVVLFID
                     EIHTLVGAGASEGAMDAANILKPALSRGDLRAIGATTLKEYQKYFENDKALERRFQTV
                     LVDEPDALDAVSILRGLKERYETHHHVRIKDEAIISAVELSQRYITDRFLPDKAIDLI
                     DEAAAKLRLEINSVPEELDELERRYRQLEIEREAIKREKDEVKLKQLSAEIAELDEQR
                     KQVRARWQAEKEVVESIQHRKKEIEQYKFEAEQAEREGNYGLVAELRYGKTRDAEKQL
                     EELKKQLAGMQASSALIKEEVGSEEIAEIVSRWTGIPVSRMLQSEREKLLHLEEELHK
                     RVIGQDEAIQAISDAIRRSRAGLQDAKRPVGSFIFLGTTGVGKTELAKALAEFLFNDE
                     NAMVRIDMSEYQERHTVSRLVGAPPGYVGYDEGGQLTEKIRRKPYSVVLFDEIEKAHP
                     DVFNILLQVLDDGRLTDNKGRTANFKNTIIIMTSNLGSNIIQENLEDITEANREAVIE
                     KTKEEVFALLKKTIRPEFLNRIDEIIMFSPLTLGEIEQVVRLQLGHVKTMLEDQDIKL
                     EVTDDAVKLIASLGYDPLFGARPLKRVLQQKVLNELSKMILSGQLNGNSTITIGEKDQ
                     EFTYHAA"
     gene            complement(4752..5390)
                     /locus_tag="PKI35_02235"
     CDS             complement(4752..5390)
                     /locus_tag="PKI35_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43226.1"
                     /translation="MKKIIPIFMMLLMCSITSTAQVEKGKWFAAGYGTVNFDFGKEKY
                     KSGGTTSESHKYFELDLNPLIGYFVIDKLPVGLYFDSYFHHYKYAYNDDKEKESQFIA
                     GPFVRYYILEMDKFFPFVEGRLGLGLWRSKYDDNDAEKYSFISPQLGIGTSYFFTDHV
                     ALDAYLGYSHQAWTDKSEHETGVKSVLADDEYKEMYGAFVFRVGVDITFGKE"
     gene            complement(5404..6003)
                     /locus_tag="PKI35_02240"
     CDS             complement(5404..6003)
                     /locus_tag="PKI35_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX43227.1"
                     /translation="MKKILLLLCLLITYSFAFSQTEKDKWFAAGYSSLRLDIGKEKYK
                     SGGTTHEYNTYRHFDFNPMVGYFIIDKLPVGLYVDLSARRVKDSDDGDIWKETEFVAG
                     PFVRYYVVQIDKLVPFVEGRIGLGAYKEDDEGDIYKESVFSYRFGAGASYFFTDNVAF
                     DSFIGYDYDRYTSVDNADEYSYFYPSVEINFGIVITFGK"
     gene            complement(6430..7833)
                     /locus_tag="PKI35_02245"
     CDS             complement(6430..7833)
                     /locus_tag="PKI35_02245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX43228.1"
                     /translation="MNKEDIRQFFEKSSGTREKWKHRNRFYHKAIEKYISFIIPEGKR
                     VLEIGCSNGDLLNATKPAYGTGIDFASGFVENARKKYPNLVFYHDDVENLKLNDTFDY
                     IILSDLVNCLWDVQTALHNIRKLCHPQTRIVISQYNYFWEPLLKTGEFLGLKLRQPAQ
                     NWFSISDIDNILQLEGYQLIKVERKLLLPKYVPILNFLFNKVLANLPLINHLNLVNFM
                     VARLVLGEDQPYSVSIVVPARNEKGNIENAVRRTPEFGKSMEFIFVEGNSSDNTFDEM
                     LRVQQAWPDKNIKVMKQSGKGKGNAVREGFEAATGEVLMILDADLTMPPEELPKFYDA
                     LRYNKGEFINGCRLVYPMEKNAMRFLNLLANKFFGWFFSFILGQRLKDTLCGTKVLYK
                     TDYQKIISNRSYFGDFDPFGDFDLLFGAAKLNLKITEIVIRYRDREYGSTQISRFKHG
                     WLLIRMSLFAARKIKFI"
     gene            7945..9951
                     /locus_tag="PKI35_02250"
     CDS             7945..9951
                     /locus_tag="PKI35_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43229.1"
                     /translation="MAKQQKSVQRVQHYGPKQASKTKVAESKTFIQSVDDFLAKRLNI
                     IFWVCMGLTLLFSLLLFDPKVSLSGDDAFYIIRASDLLKKFEYPSFQGPLYPIVLSVF
                     LAIAGLNLTILKGVSLLAMLAFVYFTHKAFKNRVPAILHVVVLALVCINSFILYYASQ
                     TYSEAFYMFLQSILVLVFFTKFIDTEQVIGKKQDVKRHIVLALVLLSLTLTRSIGFSA
                     VIAVFGYFTLKAQWRNLWMGALSFLGAYLVFQGIRYLLWGDATLQFASQGSGLLQKDY
                     YNAAKGNEDFAGIMKRFWENSNLYLSKHFFTIGGLRKFEVIQTVIPILTVLFYIIFIS
                     GLVLSYFRNRYVFFAGLLSGSFLLITFIILQSRWDQSRLIIPVFSLMVLMFFSAFYYL
                     SQTGKFRVMYWVIPVMGIIMFLQILAYTSVKSKEASQTKGKYGGLTPDWKHYLQASEW
                     AANNLPPDAVVACRKPSLSYIYGKGRNFYSIMKVPSFSSEQFFATWGKENIPYTLFRY
                     SDVVGKQLTPSIYSAIKNYYEAQLFIGEEFYIVDRYPDSINEKIRLELTQAKLPYITN
                     REDMQARVSAEKQPPVLIYPDSLLIPLIDNGVTHVLTANLRRNSLQKNGQIINTVERY
                     MAYIQDKYPDFMSPVQKIGSDEDEPAIIYKLNYERQKNFLGRKK"
     gene            complement(10193..12022)
                     /locus_tag="PKI35_02255"
     CDS             complement(10193..12022)
                     /locus_tag="PKI35_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNX43230.1"
                     /translation="MKERIRLFISYWLFWYLLFIVARIAFLVYQFHASMQLSFSELTG
                     TFIHGFTLDASMASYIMVVPALLCLLSCFFSFRLLPALLKAYTLIALLPATAIIIVDM
                     VLYSYWGFRLDTTPLFYMDNPEAMTASVSVLVIFAGIAGILLVTYGIFRLFLLLLKRF
                     RFPFAQNFKASLVVAVLTGLVAIGIRGGVGIGAVSISWAYFSDNTFANHAAINPVWNV
                     AFSLTEKDDLSRKYNILDKTTAATVCDSVFRSYGKLVPVLNQQRPNVILIIVESFTAK
                     AVGTLGGIPGITPQFDSLTHEGILFDHIYAASDRTDKGLAAVLAGYPSLPGSSPLKFP
                     QKTDDLPSFPKYMAAAGYNNSFYYGGSLDFANYRSFLVQTGFKKLITRDDFDASTFGT
                     KWGAWDHLVFERVLAETPDSDNGFFKSILTLTSHEPFAIPVPPMKKGNDKETLYLNSL
                     HYTDGCLGKFIAEARKRTWWKNTLIIITADHGSIMPGHSDVDDPQKYHIPLLFLGGAL
                     SVKDTVIHTVAGQTDLARTLMAQMNLPDTGFSFSKNILTTSPVPGAFYTYSGGLGYTD
                     GKVLFSYNTINQQFNDGNISGNAGERKIPLSFLQHLYDDFSRR"
     gene            12110..12631
                     /locus_tag="PKI35_02260"
     CDS             12110..12631
                     /locus_tag="PKI35_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43231.1"
                     /translation="MDKIRELLTGDSSRSFTDYLSSLIEKDAQLRAEFWEMFFANEEP
                     YSRRAAWALDLVCETRPGWSQPYASRLINSLPALTHDGMKRHSLHMLSRIEIPEENMA
                     GLINICFEWLLSPSEAVAAKVYCMDLLYRLSQQFPEIKHELIDSIEYGLGDGTPGYKN
                     LGRKLLVKLYQEV"
     gene            complement(12801..13433)
                     /locus_tag="PKI35_02265"
     CDS             complement(12801..13433)
                     /locus_tag="PKI35_02265"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNX43232.1"
                     /translation="MMKKILFCLVLTALTSLVYSQSDKKATAILNDLSAKTKSYKTIR
                     IDFAYTMDNKKEAIHEKFSGTLLSKGDKYKLTVAGQDVICDGKTMWTYMKDANEVNVN
                     DVNANDDSFTPTKLLTNYTKEYKAKFIQEKGNSQILELYPLARGKSFTKVKLTIDKNL
                     KQVKEFMIFDRSGSIFKYIVNKFITDQAIDDKTFVFNKASHPGVEVIDMR"
     gene            complement(13449..16031)
                     /locus_tag="PKI35_02270"
     CDS             complement(13449..16031)
                     /locus_tag="PKI35_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNX43233.1"
                     /translation="MEQKNEIKRPKNELRNEKKAGSAPEEAKGKTSRPASKKEKSSGG
                     DNNSEKAKQIIGAFLLLFSVFLCISFASYLVNWFTSRTDDIFSGINFSRVLHDDSLTA
                     NNLGGRMGAALSVLLVKQGFGIGTFFLVAWIFFAGVRASLKHTIVPIWKSLGIFILAM
                     VWLSVTIGFIFTHSKLSILGGISGYKTAQWLEGLLGEMGTAIALIFVFISVLALAYNF
                     SLHQLRNRIREINPLSQYSGEEEEAMRKAADEKSDRFNTVEYAVKENETDILVSGTAP
                     GNTIKNQDDKIDIPASGVLKETDSDGFEPVPMPAPRVSPRAEIEFMVETAAEPVKVPE
                     PEQDREQDAQHFPIDSEYDPTLDLGSFIMPSPDLLDNHNDGGISVNKAELESNKNRIV
                     ETLGNYGIQIDKIKATIGPTVTLYEIIPAPGVRISKIKNLEDDIALSLSALGIRIIAP
                     IPGKGTIGIEVPNQKPEIVGMKSILSSEKFTNSRYELPFGIGKTIQNEVFVADLTKMP
                     HILMAGATGQGKSVGLNAIITSLLYKKHPSELKFVLVDPKKVELTLFSKIERHYLAKL
                     PDSDEAIITDIRKVVRTLNSLIIEMESRYDLLKDAQVRNIKEYNAKFIARKLNPLQGH
                     RFLPYIVLVIDEFADLIMTAGKEVEVPLTRLAQLARAIGIHLVIATQRPSVNIITGTI
                     KANFPARIAFRVISKIDSRTILDTGGADQLIGRGDMLLSQGGEIIRLQCAFIDTPEVE
                     RITEFIGSQRGYPTAFSLPEYNEEEGGNNGDSELDPDERDELFEEAAYLIVAAQQGST
                     SLLQRKLKLGYNRAGRIIDQLEAAGIVGPFEGSKAREVKYATEFALEQYLKDLRNKES
                     QNGF"
     gene            complement(16185..17693)
                     /gene="lepB"
                     /locus_tag="PKI35_02275"
     CDS             complement(16185..17693)
                     /gene="lepB"
                     /locus_tag="PKI35_02275"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNX43234.1"
                     /translation="MDLMTSLIIFILVFPALLFWIFQDAGTKAWKSLIPVYNYVVWNK
                     INGKPWWWLIFMLVPFINLFMIMLMLVETAKSYGKYKLWEQAVAVLVPYAYLPYLGLS
                     EKEKYIQPDKRVRPKKTVTREWVDAIIFAVVAASIIRIFMIEAYTIPTSSMEKSLLVG
                     DFLFVSKVSYGPKLPNTPIAFPFVHHTMPLTKYAKSYSELIHWPYYRFPGLGKVKRND
                     VVVFNYPDGDTVALKMQNQSYYGLVREFGHDRVWNDSYNFGKIVSRPVDKRENYIKRC
                     LAIPGDTLEIRDQLVYINGHAADIPENSQFKYIVVTDGSPISKRVQDKLNITEEIEMT
                     EQGDYVLTLTKQSLEKIKALPNVKEVHPVVQPKGFWQPYIFPFDSTYAWNVDNFGPLY
                     IPKAGVTVPLTISNLPLYQRIIEAYELNKLEVKGNKIFINGKESNSYTFKLNYYWMMG
                     DNRHNSADSRFWGFVPEDHIVGKAVFVWLSLDSHKSLLSGKIRWNKLFRGVH"
     gene            complement(17867..18583)
                     /gene="dapB"
                     /locus_tag="PKI35_02280"
     CDS             complement(17867..18583)
                     /gene="dapB"
                     /locus_tag="PKI35_02280"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNX43235.1"
                     /translation="MKLALIGYGKMGKAVEEAALSRKHTIHVVIDNADDWKSKGHLLK
                     ECDAAIEFSIPAMAVSNMYACFDAGIPVVCGTTGWNEKAGEVKARCIEKNGALLYASN
                     FSVGVNIFFEINRRLARLMNTQDNYEVSIEEVHHTQKLDAPSGTAITLAEDIIRNIDG
                     KSKWKNEKTDNPSDLFIQSVRQGTVPGTHTVTYESENDFITIRHEAKSRKGLAAGALL
                     AAEFIAGKKGIFSMKDLLGI"
     gene            complement(18629..19225)
                     /locus_tag="PKI35_02285"
     CDS             complement(18629..19225)
                     /locus_tag="PKI35_02285"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNX43236.1"
                     /translation="MVRCRRITGWILIAVFTVLLCPFRSFAQETDTIRKVQKHAADSA
                     FVSKHSPKKAAIFALVLPGLGQAYNHKYWKMPIVYAGFGTMYYFVKFNTDNYHEFRDA
                     YSYVLSGGSGTPPNNLVNTYNQSQLLEGREYYRRNLELSYIITGGWYIIQALDAVVDA
                     HFFDYDVTDDLSLHMSPWTPPPVAGIRPAGGITLSLRF"
     gene            complement(19278..20177)
                     /locus_tag="PKI35_02290"
     CDS             complement(19278..20177)
                     /locus_tag="PKI35_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNX43237.1"
                     /translation="MNTVKKKALGRGLSAILESPETDITSKDISGNYVAGAIANIPID
                     KIEANPFQPRGEFDEEALADLMESIREQGIIQPLTIRKMGFDQYQLISGERRLRAAKL
                     AGLETIPAYIRVANDEQMLEWALIENIQRENLNAIEIAISYSRLIDECDLTQEELSKR
                     VGKNRTTISNYMRLLKLPAEVQAALRDGQISMGHARSIISVEDPLKQIAITRKINQDE
                     LSVRDVEKLVKHLDTLDGKPARPVKPEKTELPEKYNAVKQQLQERFSNKAELKRDAKG
                     KGSIVIPFTSDSDLEKIISILGL"
     gene            complement(20180..20986)
                     /locus_tag="PKI35_02295"
     CDS             complement(20180..20986)
                     /locus_tag="PKI35_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007898481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX43238.1"
                     /translation="MGKVITITNQKGGVGKTTTAINLGASLAVLERKTLIIDADPQAN
                     ATSGVGFDPKNIKTSIYECIVEEVDPRNIILKTATPNLDLLPAHIDLVGAEIEMINLP
                     NREKMMKLVIDKLRDSYDFIIIDCSPSLGLVTVNALVAADSVIVPVQCEYFALEGLGK
                     LLNTIKIVQSRLNPQLDIEGILLTMYDSRTRLSNQVVEEVKTHFQNMVFETIIYRNTR
                     LGEAPSFGESILMHDASSIGAINYLNLAREILQKNELTLLRQEDKEIKVE"
     gene            complement(21271..21603)
                     /locus_tag="PKI35_02300"
     CDS             complement(21271..21603)
                     /locus_tag="PKI35_02300"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HNX43239.1"
                     /translation="MEENDRSESREDVFSRAVKAGKRTYFFDVKRTRTEQHYITITES
                     KREFDNAQGRFIYKKHKLFLYREDFEKFGDGLKDVISFIETGIIPPERQYADNHNHAE
                     VDFEDLDK"
     gene            21800..22765
                     /gene="nusB"
                     /locus_tag="PKI35_02305"
     CDS             21800..22765
                     /gene="nusB"
                     /locus_tag="PKI35_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNX43240.1"
                     /translation="MLSRRFLRIKVLQAIYAFIQDGPESINSGEKQLVVSLDKLYELY
                     IRQLSLLVEISDFARRRIEENKLKFLPSREDLDPNTRFIDNRFINQLAANRDFLKKCE
                     SFKISWADEEEMVRKLYNQLRESKAFVAYMEGPAPSYKKDKDLAEHIFVNIFAESELL
                     ESFYEERSIYGSDDFEISFIMIQKTIKGFRADADEYTSLPTLLKDENDTDGSEDLDFV
                     KKLYRKTLIHSDEFNTIIADKAQNWELDRIALMDTILLKMAMTEFVDFPSIPVKVTLN
                     EYIELAKSFSTPKSKIFINGILDKLIVEFKANGRLVKTGRGLMED"
     gene            22887..23336
                     /locus_tag="PKI35_02310"
     CDS             22887..23336
                     /locus_tag="PKI35_02310"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX43241.1"
                     /translation="MPVPENCDSKNFTVRDYRNSDFENIQALWIPLGLGSRQRGDTAE
                     VIEQTLKAGGKLLVLEETLSGKLVGTSWMTVDGRRNYLHHFGIAETYQNKGLGRMLLD
                     ASIAFAREKGLQLKIEVHRRNQHAINLYTRNGFKYLGDYDVYIIRDI"
     gene            23391..23858
                     /locus_tag="PKI35_02315"
     CDS             23391..23858
                     /locus_tag="PKI35_02315"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNX43242.1"
                     /translation="MKPKLLLTALMAVLLITGQSCNKRGKNDLPADVIMNPNTASGKV
                     DPNALPVIEFETDFHDFGKLMSGEKVTYSFKFKNKGKGMLLISKVSTSCGCTISEYPK
                     EPLKSGAEGTIDVSFDSEGRIGLQNKTITVFSNTQPSTTMLRIQASVVDVKDM"
     gene            23941..24276
                     /gene="yajC"
                     /locus_tag="PKI35_02320"
     CDS             23941..24276
                     /gene="yajC"
                     /locus_tag="PKI35_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNX43243.1"
                     /translation="MEQLTMLLMAPAGGAQKGGSGLSSLIFLLVIFVVFYLFFIRPQV
                     KRTKEQKKYRENLQKGQKIITIGGIHGRIVEMQDTTVTIEVEGGTRLRIEKSAVAADA
                     QDQLGTEAK"
     gene            24331..25344
                     /locus_tag="PKI35_02325"
     CDS             24331..25344
                     /locus_tag="PKI35_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43244.1"
                     /translation="MMMENADRQTDTTENIVTVQPKKSFRKKFGIFVVCLGISSLMWL
                     FIELTRDYTTDLKYAVSFTNVPKDLILQNQNDSSITVGITAQGFELLTTRLFRKRHEL
                     TVDLSEIRIRKQSQGYSAYVPASKLLQQVARQIGFSKTVSYIRPDTLFFMFSDVYRKE
                     VPVRLNFSYSFEQQFQLYDSIEWSPKTVIVSSIKDVIDTIRFVSTVRTVKTDIDSSLS
                     FKIPLSRPLGRGMISYSTDSIRVRLPVQKFTETTFTIPVRIVGNTSQLKIFPDQAEIK
                     CMVPMVDYRNIEADNFLATVQADGKTLTQNPKLRINLVNVPLGVKVIKVKPEFVEYII
                     ISP"
     gene            25341..25937
                     /gene="coaE"
                     /locus_tag="PKI35_02330"
     CDS             25341..25937
                     /gene="coaE"
                     /locus_tag="PKI35_02330"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNX43245.1"
                     /translation="MIRVGLTGGIGSGKSVVAEIFRHLGVPVYHADAEAKKLYDLPDV
                     VQEISREFGSGVMDSGGTVDFKALASIVFPDPLKLQKLDSIIHPLVEAGFISWLADYR
                     DAPYTVMEAAILFESGFDHLFDKTIMVLAPVELCIRRVMARDSVTREQVGQRMKNQWD
                     PELKAERADYVLVNDERQLLLPHVIALHHTLSGLETKA"
     gene            26077..26610
                     /locus_tag="PKI35_02335"
     CDS             26077..26610
                     /locus_tag="PKI35_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43246.1"
                     /translation="MSEQILGVGSRVRHPQFGKGVVVQVRSDAYEIGFIETGVRQILR
                     DFEGMEVIEAVETPSDLATYEKIEKSLVRILRRFSDISEVTQMGQRWNNGRMVLYPGD
                     ASTKEKEVPLDAFFHKIVMVRDRLRVLEAKINASGLSDEDKVELQQYITRCYGSLTTF
                     NVLFRNKEDQFVGEKTA"
     gene            26799..27245
                     /locus_tag="PKI35_02340"
     CDS             26799..27245
                     /locus_tag="PKI35_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43247.1"
                     /translation="MKTNNYFQLIGIVFFFSVIHGCDRFDTRVKMINTSNHLVYYTWM
                     SDSSYNLLFKRINAQMESAREDSIILNRYFISVRPKIDTIEEAIPGTWEEYFANPNRK
                     IWVFIIPDSLMSQHVGEITSIQYQIKRKFFDKTYLLEHNGIIDLSD"
     gene            27507..28061
                     /locus_tag="PKI35_02345"
     CDS             27507..28061
                     /locus_tag="PKI35_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNX43248.1"
                     /translation="MSDRYKIFKGDEPYFVTFTITNWIKVLEDDSFKMVLIESIRYCQ
                     EHKGLQVYGYCIMPTHVHMMIQATGDNSVSSILRDLKTHTAKAIVRKLKNEKPDGYQE
                     ILSVFAKAGRDLKRIQNYKVWQDGNMAKMLYSNRFMLEKLNYIHNNPVKYGLCEEPWD
                     YVFSSATNYSGKTSLIDVILLSLW"
     gene            28090..28527
                     /locus_tag="PKI35_02350"
     CDS             28090..28527
                     /locus_tag="PKI35_02350"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HNX43249.1"
                     /translation="METHALQAREQRQARLNAVRKTFLDHMIKDLAPNITRKRLLLEG
                     FYTIDADKATIKDYFDYITKELNVRTYSDPIIFETGNRGKKENQGFDAFVPLIDSGIS
                     CYVWVYMKFISVIIYTCKDFDEKKAVDSTKAFFKIGKVEWNIF"
     gene            complement(28893..31298)
                     /locus_tag="PKI35_02355"
     CDS             complement(28893..31298)
                     /locus_tag="PKI35_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX43250.1"
                     /translation="MKNILLTWLFIGISGIAFSQIVTIREKETKQPIPLVTLISEAPN
                     VSAITNAEGQADISAFRDAAKIEIRSLGYKTVITSYQGLKSGNYEVLLAINNLNLDEV
                     VISATRWRQKSGNVPSRIISIPAKELTLLNPQTAADLLGASGEVFIQKSQQGGGSPMI
                     RGFATNRLLYTVDGVRMNTAIFRSGNIQNVINLDPFATENTEVLFGPGSVIYGSDAIG
                     GVMSFQTLTPQLSLTDKPLVTGKAVTRYSSANNEKTAHFDVNVGWKKWALVTSISSWD
                     FDNLRQGSNGPDEYLKPYYVQRQDGVDKVITQDDPRLQIPTAYSQINMMQKVRFKPNE
                     KWDFQYGFHFSETSSYGRYDRHNRVKNGTARYAEWNYGPQLWIMNNLNITHAGKNAVY
                     DGMSIRIAQQYFEESRIDRSLNKDERNTQTEKVEAWSVNLDFNKSLNAKNTLLYGAEF
                     VYNDVTSTGMLTDISTGTSETGPSRYPQSDWSSLAVYANDEFRVSEKCTLQAGLRYNQ
                     YILNADFDTTFYPFPFTTAEINEGALTGSIGGVYRPDETWVISANFGTAFRSPNVDDL
                     GKVFDSEPGAVTVPNPDLKAEYAYNFDLGIAKVFDDVIKLDVTGFYTILQNAMVRRNF
                     QLNGLDSILYEGELSRVQAIQNAASAYVYGIQAGLEVKLPAGFSISSDLNYQVGEEEL
                     DDGTTSPLRHAAPLYGISRLSYNTGKLSMEIYAVYQGERKFEDLPQEEQSKDEIYAKD
                     ADGNNYSPAWYTLNFKAMYPFTDRLSVSAGIENITDQRYRPYSSGISGAGRNFVFSLK
                     ASF"
     gene            complement(31401..31934)
                     /locus_tag="PKI35_02360"
     CDS             complement(31401..31934)
                     /locus_tag="PKI35_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43251.1"
                     /translation="MKSRIAGIFLIFCLVSPIITTYAWLKYKKYAVRKEVKKQIIAGI
                     DKDQLVLLKFARNESRDKLRWEHAGEFEYNGRMYDVVDTDTTANGIVYWCWCDDEESK
                     LNRQLSLLTGKILHTDPRNREQQKQISSYFKSLYFSENSGWHTALQSIATKRFTIHSN
                     RLFLFSFPPPTPPPRVS"
     gene            32402..33118
                     /locus_tag="PKI35_02365"
     CDS             32402..33118
                     /locus_tag="PKI35_02365"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNX43252.1"
                     /translation="MEKITRFFITAMVLSVMMLVTSCIDCIEGDGNMISKTLDVPAFS
                     KIKLNGDANITLTSDSTALFLIKGEKNILDEYRVEVVNKTLVIKSEHCLRSHEQVTLQ
                     IPYTELESVVINGSGSVYANETVRSESLDLKINGSGDFELPVEVQDLTGSISGSGDLL
                     LKGSAKNLEYTINGSGNIETPDLTSEQCSVTVNGSGDCSVWATGNLKVKVHGSGDVYY
                     KGNPAIQTDIKGSGSVTKKE"
     gene            33227..34267
                     /gene="porQ"
                     /locus_tag="PKI35_02370"
     CDS             33227..34267
                     /gene="porQ"
                     /locus_tag="PKI35_02370"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HNX43253.1"
                     /translation="MKAVFQTLSIALIVFLASNGYAQTGGNNAYEFLNLSNSARIAAM
                     GGNFLAVDDNDITLTQANPSLINASMNKSLALGFVDYYSDINYGFAMYCRDFKKVGNL
                     AGSLQYISYGDFKLTNEQGEVQGNFNAGEYAFNVGWGRTLDTVFRIGANAKMILSNLE
                     TYNSLGVAVDVAGSYVPDNNTCVSLLFRNIGRQITAYSSGDVEPLPFEIQLGMSHRLE
                     HMPFRVSLLLTHLEKWDLTYDEPDNQTSSVNESSSRSDVGEFADKAFRHVVIGGEFMP
                     AKFLSIRLGYNYQRRQEMTIASKTGMVGFSWGLGIRVSKFGINYSRSQYHLSGSPNYI
                     TITTNLGDWAKK"
     gene            34320..35186
                     /locus_tag="PKI35_02375"
     CDS             34320..35186
                     /locus_tag="PKI35_02375"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNX43254.1"
                     /translation="MKVTIDPDAGFCFGVSRAIEAAEKGLSAGEELYCLGDIVHNSGE
                     LERLKSKGLKIISHEDLPVLEGKKVLIRAHGEPPETFETARRYNITLIDATCPIVSKL
                     QQRIRKSHEEGLKTGGTVVIYGKPGHAEVVGLVGQTGGRAVVVRKPEDLNGIILSSPV
                     HLYSQTTMDKEGLDAMAAEIEKRLREKGNAGLFVHNTICGQVSGRAPLVRKFAKQHDV
                     VIFVSGKKSSNGAWLFSLCLSENPRCHFVSSKEDIRKEWFNAGDSVGITGATSTPPWL
                     MEEIAREISTFS"
     gene            35257..36369
                     /locus_tag="PKI35_02380"
     CDS             35257..36369
                     /locus_tag="PKI35_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNX43255.1"
                     /translation="MYLRRLQITNFKNYAEADLSFSEKINCFVGNNGEGKTNLLDAIY
                     YLSYTKSYFGNVDSQNIRHGEDFFAVHGTFARNGNTPDVVSCIQKSGQKKQFRLNKKE
                     YDRLSDHIGLFPCVMISPYDRDLINEGSDIRRKFIDMVISQFDKPYLADLIRYNRLLE
                     QRNALLRQFAELHHVDREMISLLDEQMVAPATAVFEKRRSFIADFLPIFSRYFGFISN
                     NREEVGIRYESQLQQAGYINMVNGCSERDLVMKYSTTGIHKDDLMFEIGGFPVKRFGS
                     QGQQKSFVVALRLAQYDFTKARIGYKPVLLFDDIFDKLDPSRVEQLIKLVGDENFGQV
                     FVTDTEPDRIRRIFSLLEAEHKIFNIGAGAAVEMSS"
     gene            36370..36663
                     /locus_tag="PKI35_02385"
     CDS             36370..36663
                     /locus_tag="PKI35_02385"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNX43256.1"
                     /translation="MIRKSTEILLGKAIEEWLRTNHFDEKLNETRLLSSWEKVAGKLI
                     ARHTENLFIKERELFVKVDSAALKHELTYMKSKLLKKLNKTAGAEVIEDIRFI"
     gene            36852..37379
                     /locus_tag="PKI35_02390"
     CDS             36852..37379
                     /locus_tag="PKI35_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNX43257.1"
                     /translation="MNIKNFEFKASVDNIEKYENLLLKLNPRFQGTDHQTDTYFNTGK
                     GRLKLREGNIENSLVDYNRENLQDSKKSEVILYRHAPSDSLKSILVHQFGILVVVKKI
                     RKIYFIGNVKFHFDEVENLGSFIEVEAIDDQNKFSDEDLKEQCDYYYRYFGIQKEQLQ
                     KLSYSDMLLNFENKE"
     gene            complement(37407..38018)
                     /locus_tag="PKI35_02395"
     CDS             complement(37407..38018)
                     /locus_tag="PKI35_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNX43258.1"
                     /translation="MKIICIGRNYPAHVEEMENDIPAEPLFFMKPDTALLIRNRPFYY
                     PEFTSELHYECELVLRICKLGRHIAEKFAGTYYDAVGIGIDFTARDLQREAMEKGLPW
                     ERAKSFDFSAPVSRFVPVSEFRDANSVNFSLQLNGKVVQEGNSKDMIFSFGRIISHVS
                     KFVTLRTGDYIFTGTPAGVGPVQIGDRLEGFLEGDRMLAVEIK"
     gene            complement(38039..38848)
                     /locus_tag="PKI35_02400"
     CDS             complement(38039..38848)
                     /locus_tag="PKI35_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX43259.1"
                     /translation="MQLKLTRPLAIFDLETTGTNIANDRIVEIAILKINPDGHEEIRV
                     NLVNPGMPIPAEVTAIHGIKDEDVKDKPSFKELAGNLIAYIGNSDLAGYNNIKFDVPL
                     LVEEFLRAGLEFDMKNRRVVDVQNIFHKMEPRTLKAAYKFYCGKPLENAHTAEADTRA
                     TYEVLLAQLDRYEGVEYEDRNGNISKPVVNDIKALHEFSYYSRSADLMGNVIFNDKGE
                     EVFNFGKYKGAKVEEIFAKEPSYYDWIMKSAFPLTTKKVVTAIKLRNFNKG"
     gene            39264..41210
                     /locus_tag="PKI35_02405"
     CDS             39264..41210
                     /locus_tag="PKI35_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX43260.1"
                     /translation="MKERSDKAYAGLSGSSGTTRVRLLNELVSYRLTDSADKAARLAR
                     IAVSEAGEIKDNSLLARSWVALGSVYLTKKMPDSAKYALYQAGRLFTKYSMTEGKDFY
                     YLTRGRLLLMQNREVDAIAEFRDAVPLAEKRSDYVTLGLIYKQLAACYKQLNRPKELI
                     SSLDKASFYFRKGGDPFILGPQQMGLGILYLDMGLEEKASEQFIMAEKASETASDSIS
                     LGYLYSNIAGIYYSHACRQKAEEYYENSMKIFRATRHYQGMAYCLNQMGMGYFNENQY
                     KKALPLLSRAAMLKNESSDWQGACFVYSNLVEVYIRLNKRNEATSAIKTASEMCIRAG
                     DKLSMAAFMQANGSYHTYLREYDKALGYYQRSIQLARELNVQGIVMEDLRAISEMYQL
                     KGDAGGALEYYKKYTTIKDSLAKASDFKALADLRMKYETEKKDRQLKTLLTRLKYGKE
                     RYVFLVNGIIIAVLLIIFTILFFRMRGNYSGSKQRLPALRKQTTAGPRIEATPAPLML
                     PAEKASKKVLTGEMQSVLWIRLQELMEREKLYLRNDLTLSELARKLNTNTTYLSKVIN
                     DITQQNFSNYLNNFRIDEACRLLSGPHGRNLTIEGIAQSVGFNSKSAFNAAFKKIKYV
                     TPSEYLSREQNAAVRQSLEKNPVA"
     gene            41529..41753
                     /locus_tag="PKI35_02410"
     CDS             41529..41753
                     /locus_tag="PKI35_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43261.1"
                     /translation="MLAITGIIFILFNLTGSISVTGTKAGWLFQKKEYAATVTAFSIY
                     RKAWPVSHNRFAFQVADVSGKKMIPDSFII"
     gene            41897..43582
                     /locus_tag="PKI35_02415"
     CDS             41897..43582
                     /locus_tag="PKI35_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43262.1"
                     /translation="MRLIRTLTGLFLLAVLPYAAPAAGQTANTDTGPRAYFKLDGNGI
                     DAVSGLKASILHGKVIRGHDGKEQGAYEFTINDRDYSKSTRSLTFPVDINASKWPSLT
                     ITAWVKASNYFMPMYVITNYTNNKTRGLILDKDDDMYRWGMCCGKDGMLWGPTLVEGW
                     TFVAMIYDDKNQEARLIVNDEVFSSRAKMHSGENKAFVGAFKGAIDDVRFYDRILSAA
                     EIEAISGKPIVKGADDLAIKDRYSYKERLQQEKENKLVIGGVYIVDKEEIKVFTTTNQ
                     ENTADVLRNPDSLKVLEKLPDGWYKISYGNGKTGFARRSEIVNKAYPEGSSVLKFRFM
                     NWLSNVFDFTKLKSWIFVLICAVILFYVKRKFNTLDDRLLKLRPAHDQFADGGSKSDA
                     SVPPSQSFLHRIYPVKKYRWYPLLGGILLGATIFIASFWDGNEMEWFFNEGFNILPIG
                     YDRPVHWFMYGMCMITILLTLSWIVESFVIGGPLIGLLRVFILLILNCMALLVSFFLL
                     VLIAFLVIGILVLKVLGSAAGSGKYKCPHCGRTFSASAGSNTRCPGCGASLTT"
     gene            43640..46657
                     /locus_tag="PKI35_02420"
     CDS             43640..46657
                     /locus_tag="PKI35_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX43263.1"
                     /translation="MLIDRYYFSRAFPNKEIDKCWEEGNTIISAAYGNGQWVLIAASE
                     SDLDGQQWNTQGDFPSGPINEGWNARKDINWLSYGNGAWVVFMSGNTGYSDQVWRTSS
                     RFPEKEISQGLKDGYMVTGLAYGSDRWAVVLTKGSGFTAQQYDSYRSFPKDAIQKGWD
                     NGFDITSLAFGDGKWGLVMSQGTGFGAQEWITGSEFPVNDINAKLREGYSITHIAYGA
                     GLWAVIVSETETEEDDEEEEETDETDTEDAVSDLDPEAERLHDLGIDFLEKENYDKAI
                     VNLKKAVDIEPEYADALAGLATAYTYKDDLDTAITYYEKAFALDNETPVLVSNLILTY
                     NNKGCDDKILDVVKRADPECFDKIDFAETLNIIGVCYADAGDFNNAVKFYNKALKLAP
                     GNKQIRDNLDESEESLRQPAAEEVSVPQPAVHMVPDVDNDQLLEMAMKELHAMTGLET
                     IKRDVDDLLKYIRIEKLRRERGLDANPTVLHSVFSGPPGTGKTTVARLLGKIFKALGL
                     LSKGHVVEVDRSGLVGEFIGQTAQKANKVIDSALDGILFIDEAYSLAPEDDPRDFGRE
                     AIETLLKRMEDSRDRMIVIVGGYTEEMKRFIDANPGLQSRFTRYFYFDDYKPEQLSEI
                     FSEICVSRKFTMDDDARAKLLRYTEFLYKSRTKNFGNARTMRNLFEEVIRLQSGRLAM
                     LSQISDNDLCAITEADITEAVKDEFADEKQETIADVLKDLEELVGLENVKKDINTLIN
                     YLRIEKLRQEKGMVANPVSLHTVFFGPPGTGKTTVARLLGRIFKVMGLISRGHVVEVS
                     RADLVGQYVGQTAPRTMKVIDSALHGILFIDEAYMLTPANGGNDFGQEAVDTLLKRME
                     DDRDRLIVVVAGYTNEMNHFLESNPGMKSRFNRHFYFNDYSPEELTDIFCKMCESRHF
                     ILEEGVKGIALEYMQQCWDERDKTFGNGRMVRNFFEKLVQAHSDRLSTIADLDENSLV
                     TFTQADIRRVVGLGGAEKSGSKSIGFKNNSFKS"
     gene            46654..47886
                     /locus_tag="PKI35_02425"
     CDS             46654..47886
                     /locus_tag="PKI35_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43264.1"
                     /translation="MIKYLENRVWSLNAKIVNTEADPAEINRLLAEKLTSGIFAGEGI
                     RFSYSENPIGNYSFRPVEVTLEMTAKAIGQYQQFLDEKGISFRISSFRMIINAFGFLN
                     IIPVFEYACKPGNNSYAEIEKNGDASAEIIDAIFSDISAAIDVLDSAGIIRKSSYYHF
                     GVPSGLEKSGIHTREDSYNYLVHIFYFGEKELLDDTVKQFNSEENVLTFEEHCLFSEF
                     PIYFWEMKHDVADNELVDLISINSYMLCETVAVNNALHVYNSFLDILNQNLEVDSNHL
                     REIFNYNTWLIQNLRLFNPNFTLYQSKFMKMYRQNSDISPKYDLLKDAEQSLTFAIEG
                     IEVTRTQQSERIMQFILALFTALTLYSVITDAYALITSEVQAVPFSMHSTQTIIFILE
                     TTVVLAFIIFFRKISRRI"
     gene            48166..50487
                     /locus_tag="PKI35_02430"
     CDS             48166..50487
                     /locus_tag="PKI35_02430"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNX43265.1"
                     /translation="MRFSGMRSMLLWSGLAFLSLVFGSRQAGAQVTPEKSYSDYVNPF
                     IGTQGEGNTYPAAVAPFGMIQIGPDTDIERWETASGYEYSDSLLYGFSLTHFSGTGIP
                     DFGDFLFVPSTGKIKTYGGKRDAWNAGSYRTRFSHSQESAAPGYYSVRLPEYDINAEM
                     TASDRAGFLRFTFPQTDSANIMIDLKHVLQWKVVWSQVRVMDDSTITGYHYVKGWARD
                     RKVYFAARFSKKFERFGIYRNGRVVEYDPFKTYRFVSSNEAADTNLQFFAAYKTAAGE
                     AITVKVAVSGVSSDNALLNMNTEIPHWDFSRVKEETRDKWSRELKRIEVEGTDDEKTT
                     FYSSMYHLFLTPFLYQDADGSYLGMDKNIHKAQGFTNYTVFSLWDTYRAVHPLFMLIQ
                     NERNADMINSMLKHYEQSADHLLPVWSFYGNETWCMIGYHAVPVIADAVVKGLKGIDA
                     AKCYDAVKATAMNREYDNVMLYAKLGYLPFDVENESVSKTLEYGYDDYCVARMARYLG
                     KTDDYNYFMNRSKAYRNLFDQQTGYMRGKDSKGNRRTPFSPDDYSDDLRDRDFTEGTS
                     RQYSWYVPHDVQGLAGLMGKEKFEARLDTLFALNVPENNQTAEDATGRIGEYWHGNEP
                     SHHIIFLYDYIGKPWKAQKLVHKVVSTQYGSKPNSLCGNDDCGQMSAWYIFNALGFYP
                     VCPSGSYYAVGSPCLPKAVVNLSSGGKLTIRANNLSKDNIYIRSMTLNGKDWNKTYIP
                     YEEIRNGGEIVFNMTNKPNRKWGTAPGSLPPEE"
     gene            50581..51228
                     /locus_tag="PKI35_02435"
     CDS             50581..51228
                     /locus_tag="PKI35_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43266.1"
                     /translation="MCFSATASFTAGAVITAIGVASELKVRKKSQRLFAAVPLIFGLQ
                     QIAEGFVWVSLQRPDQLLMQYISTYVFLITADVLWPVMVAASCLLMEENPGKRKILKI
                     LLAAGAALSLYYAFCLLSFAVTPEILNCHINYAGEFPHVLVIPAFLVYLVVTITPFFV
                     SGVKGMPWLGAVLFLACVVSVIFYIQNLTSVWCFFAAILSIFIYLLISREKQKGS"
     gene            51663..51851
                     /locus_tag="PKI35_02440"
     CDS             51663..51851
                     /locus_tag="PKI35_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43267.1"
                     /translation="MATRQIDNKIILKEIENPINDLINLQTVYDKTKIATTKTELKTK
                     YLNALILAANKSGLKLKK"
     gene            52016..52561
                     /locus_tag="PKI35_02445"
     CDS             52016..52561
                     /locus_tag="PKI35_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43268.1"
                     /translation="MKKSDIYEIALKILGVYLFIHIVALLRTIFSFLSYYDIARYSPA
                     QIDKYSPMPELMASVIQLVIDVTLAYLLTFRTKRMTHLICSQQDYTETVQLITEPKYL
                     YQIAMVLVGLSAIVRTFPDFLIKLQHHIHKVQLDDHSYLYDINYLLTEAIELLLGFLL
                     IYYNRPISEILTKDKKKRVAE"
     gene            52754..54967
                     /locus_tag="PKI35_02450"
     CDS             52754..54967
                     /locus_tag="PKI35_02450"
                     /inference="COORDINATES: protein motif:HMM:NF016945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase"
                     /protein_id="HNX43269.1"
                     /translation="MKYTSLFLLLFLFFGLKPVKANEPEHQSIYQLIERILPGKSSQF
                     VIGDLKTAGNTEAFELSGKNGKIIINGTGELAIARGFNWYLNHYCHTRVSWYKDEPVN
                     APAVLPAVDTTVMQECRFEKRFFLNYCTFGYTMLWWQWDDWERFIDWMALNGINMPLA
                     ITGQEAVWMEVWKSFGMSEDQIRAYFTGPAHLPWHRMGNLDGFLGPLPQNYIDHQTEL
                     QKKILKRERSFGMTPVLPAFAGHVPKAIKEKFPDAKITSMGSYGTGEAYDAFFLDPMD
                     PLFIKIQQKFLKIQTEYFGTDHYYGADPFNEMDPPDTTPQYLGSVSKSIYEGMHNIDP
                     EATWVQMGWTFYYMDLWKSDPACLKAMITAVPENRMIILDYFTEQEEVWRNTKAWHGA
                     PYIWCYLGNFGGNTEMAAPLKEIAGRLPKTENDTTHGKLYGIGSTLEGFNVNRFMFEW
                     LFEYAWDKNVTNVDQWITGYAKTRTQNNDPVSEEAYRKLIGLVYNKQISNIGIGSLMQ
                     TRPLLTGFKGYQRPNKYNYRELTDILTLMLSANEKSLQSAAYQKDMVVVTKQILDNLI
                     IPLRKKINAAYVNKDTVELDKQAKIFLGVMDDQDRLLATQPEFLLGKWIHDARQFGND
                     AASKAYYEKDARVLITTWGNEGNEIIDYASRDLSGLIATYYKVRWEMFFEKLKMNIRN
                     NGTLDMEAVNREMASFEWNWTSQQQPFISVPQGNALEMVREIHKKYSSLFSICND"
BASE COUNT        14598 a        12398 c        13786 g        14205 t
ORIGIN      
        1 ttccatcgtt accaaaggag ttttgatttg ttttaaggct ggctcaggct ttcattcccg
       61 gtatcttcag cattatcctg ccatgtagaa aaccgatacg gtatattaaa atgttgatcc
      121 cggatgcctg aacggtacaa cacaggattc cgatgctcaa aaataagaaa acaaaattat
      181 gcgggcatta attttcaaaa cagggatgtg ttttttaatt ataaaattcc tgttacgcgg
      241 gacaaaatgc gggcttcgtt aaccggcagg aacttcctgt tttcggaaaa gcagggccgg
      301 ggagaatata cggaaggacg gaaatacgcg ggattgctga ttatcaggct gccggcggcg
      361 tggttttcaa ctttcccacc tgccatctga ggaccagcag ggtgaggagg gaggaagaaa
      421 agtcggcgac cggggcgctg agccagacgc cgttcagtcc gaaaaaatgc ggcatcacga
      481 gaagcgccgg gatcaggaag agcacctgcc gggtaaggga cagaaaaatg gatatcctgg
      541 ctttaccgat cgactggaag aagttggagg tcacaatctg gaagcctacc acgggaaaca
      601 taatgaggtt gagcctcatg cccagggatg ccaggccgat gagttcatcg ctggtggtga
      661 aagccgaaga tatctgccgg gggaatattt cggccagcag gaatccgaag gtggttacgg
      721 caatgccggc gatcacggtc agtttgtaag ccctgatgac gcgcggtgtg cgccgggccc
      781 cgaaattata gcccaccaca ggctgcattc cctgggtgaa gccaaggaca accatcacca
      841 cgaggctccc tatgctgttg atgatgccga aagcgccgat ggcaaagtcg ccgccatgtt
      901 tgcgcagcga gaggtttacg atggaagtta cgacgctggc gcagatgagc atcaggaagt
      961 tcgacatgcc tatggagata atatcccgga tgatcttctt tttcatttta aaatagccgg
     1021 ggaggaagcg cacaaaactc gatggttttg tgaagtgggt aaaggagacc gccgttccgg
     1081 tgatttgcgc ggttaccgta gccagggcgg cgccccgtat cccccatttg aaaacgaaga
     1141 taaaaagcgg ggcaaggcaa aggttgatgc ccacggtgat gagtgtaacg gtcatggctt
     1201 tgccggggta tccggaggcg cgtatcacgt tgttgaggcc aaggtaaaga tgggtaaaca
     1261 cattgcccag caggatcacc tgcataaatt gcctggcata gggaagtgtg tcgggactgg
     1321 cgccgagggc ataaaggacc ggttccagga aaacaaatac gaccaggccg acggcaatgc
     1381 ccagtataag gttgagcatc acggcgttgc cgaggatgcg ggtggctccc tgccggtcgt
     1441 gttgtccgag ccggatggaa accatggcgc tggcgccaac gcctaccata gccccgaatg
     1501 cggccgtgat gttcatcagc ggaaaggtga gcgctaatcc cgagatagcc agcggcccca
     1561 caccctggcc gatgaaaatc ctgtcgataa tgttgtaaag cgaggatgcc gatgtggcaa
     1621 taatggcagg aacggagtac tgcaagagca actttcctac tttttcaact cctaacccgc
     1681 tggcactccc ttgtttacgc attttaccgg ttccttcttt tttggggtgc aaagttcctc
     1741 attttgattg aattagcatt accggtagct aaattcttgt cagaatatca ttaaatgcct
     1801 gaataaagga gccttatacc ttctgtcatg aagcccgtgc ccaacataat gagcctgttt
     1861 accggaaagc gaaaacaccg gatgggggat tcaaccgcca ccctgtcacc tcggggcgaa
     1921 tacatctgac aaaacatcat atatatatgc gttttgggat gcgggttcgg tttttgatgg
     1981 cgagtggccg aaagtcagaa aggagaataa tcatgaattt tgaacaattt actatcaagt
     2041 cgcaggaagc catccgacag gcgcaggaaa tagcacagca gatgcagcat cagtctattg
     2101 aaggtattca cctgctgaag gccctgatgg aaggggacga cagcgttacg ccgttcctgt
     2161 tgggaaaaac gggtgtgaat acggggacgc tgaaccggga gatggaccgt ttgctgcagt
     2221 cgttaccgaa agtatcggga ggagagcaat atttctcaaa cggactttcc ggcgttttcc
     2281 ggaaagccgt tgccctcacc agagatttta aggatgaatt tgtttccatt gaattgctgt
     2341 tgtatgcctt gcttataagc ggcgacgcag catcttccgc gcttaagtcg gcgggcctca
     2401 ccgaaaaggg attaaaagcc gccattgccg aactgaggaa ggatacaaat gtgaacagcg
     2461 ttacagcaga agataattac aattctttag gcaaatatgc ccgcaacctg aatcaactgg
     2521 cccgtgccgg caaactcgat ccggtgatcg ggcgcgacga agagatcagg agggtgttgc
     2581 agatactctc ccggcgtacc aagaacaacc ctatccttat tggtgaaccc ggcgtaggca
     2641 aaacggctat tgccgaaggt ctggctcaca ggatcattaa cggtgacgtt cccgaaaacc
     2701 tgaaaaccaa gcagcttttt tcgctcgata tgggggcttt gatcgccggg gcaaaatata
     2761 agggggaatt tgaggagagg ctgaaaagcg tcgtgaagga agtgatttcc tcgaacggcg
     2821 aggtggttct tttcatcgat gaaatccaca cgctggtagg ggcgggggct agcgaaggag
     2881 ccatggatgc cgccaacatc ctgaaaccgg ccctgtcgcg gggcgatttg cgcgccatcg
     2941 gcgccaccac cctcaaggaa taccagaaat atttcgagaa cgataaagcc ctcgaacgca
     3001 ggttccagac ggtgctggtg gatgaacccg atgccctgga cgccgtttcg atcctgcgcg
     3061 ggctaaagga acgctatgaa acgcatcatc atgtgcggat caaggacgag gccatcattt
     3121 cggccgtgga actgtcgcaa cggtacatta ccgaccgttt ccttcccgac aaggccattg
     3181 acctgatcga cgaagccgct gccaaactga ggcttgagat caactcggtg cctgaggaac
     3241 ttgacgaact ggaacgccgg tatcgccagt tggagataga gcgtgaagcc atcaaacgtg
     3301 aaaaggatga agtgaagctg aaacagttga gcgccgaaat tgccgaactt gatgagcagc
     3361 gcaagcaggt gcgtgcccgt tggcaggctg agaaagaggt ggttgagtcg atacagcacc
     3421 ggaaaaaaga gatagaacag tataaatttg aagccgaaca ggctgaacgc gaaggtaact
     3481 acgggcttgt ggccgagttg aggtacggaa aaaccaggga tgccgaaaag caactggaag
     3541 agttgaaaaa gcaactggcc gggatgcagg cgtcgtcggc gctgatcaag gaagaggtag
     3601 gatcggaaga gattgccgag attgtttcgc gctggacggg cattcccgtc agccgtatgt
     3661 tacagagcga acgcgaaaag ctgttgcacc tcgaagaaga actgcacaag cgtgtgatag
     3721 gacaggacga agccatacaa gccataagcg atgccattcg ccgcagccgt gccggcttgc
     3781 aggatgcgaa acgtccggta gggtcgttca tcttcctggg tacgaccggc gtcgggaaaa
     3841 cggaattggc caaggcgctg gctgaattcc tgttcaacga tgaaaatgcc atggtgcgta
     3901 tcgacatgag cgaataccag gaacgtcata ccgtgtcgcg tctcgtcggg gcgcctccgg
     3961 gctatgtggg ttatgacgag ggcgggcagc taaccgaaaa gatcagaaga aaaccataca
     4021 gcgttgtgtt gttcgatgaa attgaaaagg cgcatcccga tgtcttcaat atcctccttc
     4081 aggtactgga cgacggcagg cttaccgata acaaaggccg taccgccaac ttcaagaata
     4141 ccattatcat tatgaccagc aacctgggat cgaacatcat tcaggaaaac cttgaagata
     4201 ttacagaggc caaccgtgaa gctgtgatcg aaaagacaaa ggaggaggtg tttgcgcttc
     4261 tgaaaaagac gatccgcccg gaattcctga accgtatcga cgagatcatt atgttcagtc
     4321 cgctgacgct tggagaaata gaacaggtcg tgcgcctgca gctagggcac gttaaaacta
     4381 tgctcgaaga tcaggacata aaacttgagg taacggatga tgctgtcaaa ctgattgctt
     4441 ccctgggcta tgatccgttg tttggcgccc gtccgctgaa aagggtgttg cagcagaaag
     4501 tgctcaacga actgtcgaaa atgattctca gcgggcaatt gaacggcaat tccacgatca
     4561 ccatcgggga aaaagatcag gaatttacct atcacgcagc gtagcctggg atgataaaca
     4621 acgcgcgttt gataagattt atctgaaaac ggatcatcgt tctgacggtt gatttcgtta
     4681 gaaggaacac ttaaaaaaaa caagccggcg gccttcgggc aaccggctta ttcatttcca
     4741 accggcatca gctattcctt accgaatgta atgtcaacac caacccggaa cacgaatgcg
     4801 ccgtacattt ccttgtattc atcatcagcc agcacactct tcacgcctgt ttcatgttcc
     4861 gatttatcgg tccaggcctg atggctgtaa ccgaggtagg catccagggc gacatgatcg
     4921 gtgaagaaat aggatgttcc aatgcccagt tgcggggata tgaatgagta tttttccgca
     4981 tcattatcat cgtatttcga tctccataag ccgagtccaa gccttccttc cacaaagggg
     5041 aaaaacttgt ccatctccag tatataatac cttacaaaag gtccggcaat gaattggctt
     5101 tctttttctt tatcatcgtt gtaagcgtat ttataatggt ggaagtatga atcgaagtac
     5161 aatcctaccg gcagcttatc gataacgaaa tagccgatga gcggattaag atcgagttca
     5221 aaatacttgt ggctttcgga ggtagtgcct cccgatttgt atttttcctt gccgaagtcg
     5281 aaattcacag tgccgtaacc ggctgcgaac catttgcctt tttccacctg tgccgtggat
     5341 gtaatgctgc acatcagcaa catcataaat atgggtatga tctttttcat ggctgcagct
     5401 ttattatttt ccgaaagtaa tgacgatgcc gaagttgatc tccactgagg ggtagaagta
     5461 ggagtactca tcagcgttat ctactgaagt gtagcggtcg taatcgtagc cgatgaacga
     5521 atcaaaggcg acgttgtcgg tgaaaaagta ggaggctccg gcgccaaagc ggtatgagaa
     5581 aacggattcc ttgtagatat cgccctcatc gtcttctttg taagcgccca ggcctatgcg
     5641 tccttcaacg aaaggaacca gtttgtcgat ttgcacgaca taataacgga caaacggccc
     5701 tgcaacaaat tcggtttctt tccagatatc accgtcgtcg gaatctttta cccgccgggc
     5761 ggaaagatca acgtataacc cgactggcaa cttatcgatg ataaaatagc cgaccatggg
     5821 attgaagtca aaatggcggt atgtattata ttcatgggta gtgccgcctg atttgtattt
     5881 ttccttcccg atatcgagcc ggaggctgct gtagccggcc gcaaaccact tgtccttttc
     5941 ggtctgggaa aaggcaaagc tgtatgtgat cagcaaacat aacaagagta aaattttttt
     6001 catggctttg gttatttggt taatattccg aaatatacga ataaaaaagt aaatttaata
     6061 aatatgataa acaaatgaat attaatttta tgctgtttgg cccttgttcg ggaaaaagct
     6121 ttcaaaaacg tgccggtttt ttataatgga agggaataac caggcccggc aatcataata
     6181 tgagcgccgt tttttaaaga cagtcctgcg gataaagaga tgataatttc agataaatgt
     6241 ttgaatgcat gcttttaaac acgaaaaagc gtccggatgc aaaaaccgga aaaccgcaaa
     6301 ccggagacca gaggatgcaa catgcaggtt taggagcata tcctgaaaat tagaaaagca
     6361 aaatgccatg agccggagac agtgtgccgg aaggctgccg gctactttat gcagccagat
     6421 cccctgtcat cagataaact tgattttacg ggcggcaaag aggctcatcc tgataagcag
     6481 ccacccgtgt ttgaaccggc tgatctgtgt gctgccgtat tcgcggtcac gataacggat
     6541 gacaatctcg gtaattttca ggttcagttt ggcggcgccg aacaacaggt cgaaatctcc
     6601 aaaggggtcg aaatcgccga aatagctccg gttgctgata attttctgat aatcggtttt
     6661 gtacagcact tttgtcccgc aaagggtatc tttcagtctc tgtccgagga tgaaagagaa
     6721 aaaccagccg aagaatttat tggcaagcag gttgaggaag cgcatggcgt ttttctccat
     6781 cggatagacc aaccggcagc cgttgatgaa ctctcctttg ttgtaccgaa gggcgtcgta
     6841 aaacttgggc agctcttcgg gcggcatggt aaggtcggca tccaggatca tcagcacctc
     6901 gccggtggcg gcctcaaagc cttcgcgaac ggcattgcct tttcccttgc cgctctgctt
     6961 catgaccttg atatttttat ccggccatgc ctgctgaacg cggagcattt catcaaacgt
     7021 attgtcagag gagttgcctt caacgaagat aaattccatg ctttttccga attccggcgt
     7081 acgcctgacg gcgttttcaa tattgccctt ttcgttgcgc gccggcacaa caatgcttac
     7141 gctgtatggc tggtcttcgc caagcaccag cctggctacc atgaagttga caaggttcag
     7201 gtggttgatg aggggtaggt tggccagcac cttgttgaac aggaagttca gaatgggcac
     7261 atatttcgga aggaggagct tacgttcaac ttttatcagt tggtagcctt cgagttgcag
     7321 gatattatca atgtcggaaa tggaaaacca gttctgggcc ggttgccgga gtttcaagcc
     7381 cagaaattcg ccggttttaa gcagtggttc ccaaaagtaa ttgtactgtg aaatcacgat
     7441 ccgggtttga ggatggcaaa gtttgcggat gttgtgcaag gctgtctgca catcccacag
     7501 gcaattgacc aggtcggata ggatgatata atcgaaagta tcgttcagct tcagattttc
     7561 tacatcatcg tggtaaaaaa caaggttcgg gtactttttc cttgcatttt caacgaaacc
     7621 ggaagcgaaa tcaatgcctg ttccgtatgc cggttttgtg gcgttcagga ggtcgccgtt
     7681 gctgcagccg atctcaagaa cccgtttccc ttcggggatg ataaatgaaa tatacttctc
     7741 aatagctttg tgatagaacc tgttacggtg tttccacttc tcacgggttc cggatgattt
     7801 ttcgaaaaat tgacggatgt cttccttgtt catattttta ataagattgg tgcaagttta
     7861 gcaaaaaaac ctaacgtatt tttaataaat agctgaaatt tgcatattcg tgaatgagta
     7921 tacatagata aacattactc ccttatggct aaacaacaaa agtcggtgca acgggttcag
     7981 cattacgggc ccaaacaggc atccaaaaca aaggttgcag aaagcaagac attcatacaa
     8041 tctgttgatg atttcctggc gaaacgcctg aatattatct tctgggtttg catggggctg
     8101 acgctgcttt tttcactgct gctgttcgat cctaaagtaa gcctttcggg cgatgatgcc
     8161 ttctacatta tccgggcttc cgacctgctt aagaagtttg agtacccctc gttccagggg
     8221 cctttgtatc ccattgtact gagcgttttt ttggctattg ccgggctgaa cctcacaatc
     8281 ctgaaaggcg tttcgctgct tgccatgctg gcttttgtct acttcacgca taaagcattt
     8341 aaaaaccgtg taccggccat tcttcacgtg gttgtgctgg ccctggtttg tatcaattca
     8401 ttcattcttt attatgccag ccagacgtac agcgaagcgt tctacatgtt ccttcaatcg
     8461 atactggtcc tggtgttttt tacaaaattc atcgatacgg aacaggttat aggtaaaaag
     8521 caggatgtga aacggcatat tgtgctggcg ctggtattgc tgtcattgac attaaccagg
     8581 agtataggat tttctgctgt aatagctgtg tttggttatt tcacccttaa ggcgcagtgg
     8641 cgcaaccttt ggatgggggc gttgtcgttt ttaggggcat accttgtatt tcagggcatc
     8701 aggtacctgc tgtggggcga tgcaaccctg cagtttgcct cccagggctc agggctgctg
     8761 caaaaggatt attacaatgc agccaaaggc aacgaagatt ttgccgggat catgaagagg
     8821 ttctgggaaa attcaaacct ttacctgtca aaacatttct tcaccatcgg cggtttaagg
     8881 aaatttgagg tgatacaaac agtaatacct atcctcaccg tcctcttcta tatcatcttc
     8941 atttcagggc tggtcctgtc atatttcagg aaccgttacg tgttctttgc agggctcttg
     9001 tcaggatcat ttctcctgat cactttcatc atcctgcaaa gccgctggga ccagagccgg
     9061 cttatcatcc ccgttttttc gctcatggtt ctcatgtttt tctccgcttt ttattatctg
     9121 tcgcagaccg ggaaattcag ggtgatgtat tgggtgatac ccgtgatggg gatcattatg
     9181 tttttacaga tactggccta cacttcagta aaatcgaagg aagccagcca gaccaaaggc
     9241 aaatacggcg gactgacgcc cgactggaag cattacctgc aggcaagcga atgggccgcc
     9301 aacaacctgc cgccggatgc tgtggttgcc tgccggaagc cttctctttc atacatttac
     9361 gggaaaggaa gaaattttta cagcatcatg aaagttcctt ccttcagttc ggaacagttc
     9421 tttgcaacct ggggaaagga aaatattccg tacacgctat ttcgttacag cgatgtcgtg
     9481 ggtaagcaac ttacgccttc gatttattcg gctataaaaa actattatga agcgcagctt
     9541 tttatcgggg aggaatttta tattgttgac cgttatcctg acagtataaa cgaaaagatt
     9601 cgtcttgaac ttacccaggc gaaacttccc tatattacaa accgggaaga tatgcaggcg
     9661 agggtatcgg cggagaagca gcctccggtg ctcatatatc ccgattcgtt gctcatccct
     9721 cttatcgata atggcgtcac gcatgtactg accgcaaacc tgcgacggaa ttcattgcag
     9781 aaaaacgggc agatcatcaa cacggttgaa aggtatatgg cgtatattca ggataagtac
     9841 cctgatttta tgagtcctgt tcagaaaata ggttctgacg aagatgagcc ggccatcata
     9901 tataaactga attacgagcg tcagaaaaat ttcctgggca ggaagaaata agagccggct
     9961 tttttttggt ggcagtcgga tgatttctga cggctgcctg acggtgttgt taagccccga
    10021 aggggcttaa ttcccccccc cagcacattt ctcacccgtt tgcctgaatt tttcgccggg
    10081 caattgccgc aagaccgggt tttaaagagt tttatggaag gacgatgtta agccccgaag
    10141 gggcttaaca ccctataccg caaagcaaca tttgtttgct gctcctgtca gcttaccgcc
    10201 ggctgaaatc gtcgtacagg tgctgtaaaa acgacagagg aattttcctt tccccggcat
    10261 tgccagaaat atttccgtcg ttgaactgct ggtttatcgt attgtaggaa aaaaggacct
    10321 tgccatcggt gtaacccagc ccgcctgagt atgtataaaa agcgcccggc accggcgaag
    10381 tggtcaggat gtttttacta aaagaaaagc cggtgtcggg aaggttcatt tgggccatca
    10441 gggtccttgc caggtcggtt tgtccggcaa cagtgtgtat cacggtatct ttcaccgata
    10501 atgcgccgcc caggaacagc aagggaatgt ggtatttctg agggtcgtca acatcactgt
    10561 ggccgggcat tatactgcca tgatcggcgg ttatgatgat gagcgtgttt ttccaccagg
    10621 tccgtttcct ggcttcggcg atgaatttcc cgaggcagcc atcggtataa tgcagggagt
    10681 tcagatacag ggtttcctta tcatttcctt ttttcatcgg gggaacaggt atggcaaagg
    10741 gctcatggct tgtcaaagtc aggatcgatt taaagaaacc gttatcggaa tccggcgttt
    10801 cggcaagtac gcgttcgaag acaaggtggt cccaggcacc ccattttgta ccgaaagttg
    10861 aggcgtcaaa atcgtcgcgg gtgatcagtt tcttaaaccc tgtttgcacc aggaatgagc
    10921 ggtaattggc aaaatcaaga gaaccgccgt aataaaagga attattataa cccgcagcag
    10981 ccatgtattt cgggaaggag gggaggtcgt cggttttctg agggaacttg agcgggcttg
    11041 acccaggtaa cgacgggtat ccggcaagga cggcggcaag acctttatcc gtccggtcgg
    11101 aagcggcata gatatggtca aataaaatac cctcatgtgt cagcgaatca aactgcggcg
    11161 ttatgccggg tattccgcca agagtgccga cggctttggc ggtaaaactt tcaacgatga
    11221 taaggatcac attcgggcgc tgttggttca ggacagggac caacttgccg tagctcctga
    11281 acacggaatc acagacggtg gcggctgtcg tcttgtccag aatattatat tttcttgaaa
    11341 ggtcatcttt ttcggtaagg gagaaagcta cattccacac cggattaatg gcggcatggt
    11401 tggcaaaggt gttgtccgaa aaataggccc aactgatgct tacagcgccg attcccacgc
    11461 ctccacggat gccgatcgcc accaggcctg tcagcacagc aacaacaagg gaagctttga
    11521 aattctgtgc aaacggaaac cggaacctct tcagcagcag caggaacagc ctgaaaatgc
    11581 cataagttac aagcaggatg ccggcaatgc cggcgaaaat aaccagcacg cttaccgatg
    11641 ccgtcatggc ttccggatta tccatataaa aaagaggcgt tgtgtcgagg cggaatcccc
    11701 agtaactgta caacaccata tcaacgatga tgatggctgt ggcaggaagc agcgcaataa
    11761 gcgtataggc tttcagcagg gcaggcaaca gacggaaact gaaaaagcag gaaagaaggc
    11821 atagcagggc cggaaccacc atgatatagg atgccatcga cgcatcaagg gtaaagccat
    11881 ggatgaaggt tccggtcaac tcgctgaacg aaagttgcat ggaagcatgg aactggtaaa
    11941 cgaggaatgc gatcctggcg acgatgaaca gcaggtacca gaaaagccag taggagatga
    12001 ataggcgtat gcgttctttc attattttag cttcaaaatc aaagcgcaaa ggtcacaaaa
    12061 atccggattt gaaatcagcg ggttaaaatt ctctctaaga actgatttta tggataaaat
    12121 acgggaactt ttaaccggtg attcgtccag gtcgtttacc gactacttgt catccctgat
    12181 tgaaaaggat gcccagctcc gagctgaatt ctgggaaatg ttttttgcca atgaagagcc
    12241 ctactcgcga agggccgcct gggccctgga tctggtatgc gaaacccgtc ccggctggtc
    12301 ccagccttat gcttcgaggc ttattaattc gttgcccgcc cttacccacg acggtatgaa
    12361 aagacattcg ctgcatatgc tcagccggat tgaaataccg gaagagaata tggccggatt
    12421 gatcaatatt tgttttgaat ggttgttatc cccatcggag gctgttgcgg caaaggtgta
    12481 ctgcatggat ctgctttaca ggctttcgca gcaatttccg gaaataaaac atgaactcat
    12541 cgattctatt gaatacggac tcggggatgg aactccggga tacaagaacc tcgggcgtaa
    12601 actgcttgtt aagttatacc aggaggttta acgggattga gaagatcggg aatattgcag
    12661 ggcgtatgaa ctgctgctct ttaccatcct tcatccctta tttccggtat ggattccgct
    12721 ccttcagaaa acgctccagc aaataaatgc cgggcctgct gaaagcaggc ccggccatga
    12781 tatagcttta gctgacaacc ttatctcata tcaatcactt ccactccggg atggctggct
    12841 ttgttgaaaa cgaaggtttt gtcgtcgatg gcctggtcgg tgatgaactt attcacgata
    12901 tatttgaaga tgctgccgct gcggtcgaaa atcatgaatt ccttaacctg tttcaggttt
    12961 ttatcaatgg taagtttcac ttttgtaaat gattttcccc ttgccaacgg atagagttcc
    13021 agaatctggc tgtttccttt ttcctggatg aatttagcct tgtattcctt ggtatagttg
    13081 gtgagcagtt ttgtgggggt aaatgaatcg tcgttggcat tcacgtcatt gacgttcact
    13141 tcattggcat ctttcatgta ggtccacatc gttttgccgt cgcagatgac atcctggcct
    13201 gctaccgtca gcttatattt atcgcctttt gaaagaagcg tgcctgaaaa tttttcatgg
    13261 atggcctctt ttttattgtc catggtatag gcgaaatcaa tcctgatggt cttataggat
    13321 ttcgttttgg ctgaaaggtc attgagaatg gcggttgctt tcttatcgct ttgcgaataa
    13381 accagggagg tcagagctgt cagtacgagg caaaaaagaa tttttttcat catgtagtgt
    13441 agtttatttt aaaatccgtt ttgtgattct ttattcctga ggtctttcaa atattgttcc
    13501 aaagcaaact cggtagcata ttttacttcg cgggctttgc tcccttcgaa aggtccgacg
    13561 atcccggcgg cttccagttg atcgatgatg cggccggcgc ggttatatcc gagttttagc
    13621 ttgcgctgca ataaggaagt tgagccctgc tgggctgcga cgatcagata ggcagcctct
    13681 tcgaagagtt cgtcgcgttc gtcggggtca agttcgctgt cgccgttgtt gccgccttct
    13741 tcctcattat actccggtaa cgaaaatgcc gtaggataac cgcgctgcga accgataaac
    13801 tcggtaatac gctcaacttc gggggtgtcg ataaaggcgc attgcagcct gatgatttca
    13861 ccgccctgcg atagcagcat atcgccgcgt cctatcaact ggtcggcccc gccggtgtca
    13921 aggatagtgc gggagtcgat cttggagatg acgcgaaaag caatacgggc agggaaattc
    13981 gctttgatgg ttcctgtgat gatatttacg ctcgggcgct gggtggcgat aaccaggtga
    14041 atgccgatgg cgcgggcaag ctgcgcaagg cgcgtcagcg gcacttcaac ttccttgcct
    14101 gccgtcatga taaggtcggc aaactcgtcg atcaccagaa caatatacgg cagaaagcgg
    14161 tgcccctgta aagggttaag tttccgggcg ataaatttcg cgttgtattc cttgatgtta
    14221 cgcacctggg catctttcag caggtcgtac ctgctttcca tttcaatgat gagggaattc
    14281 agggtacgga ctactttccg gatatcggtg ataatggctt catccgaatc ggggagtttg
    14341 gcaaggtaat gccgctcgat tttcgagaag agtgtaagct cgactttctt ggggtcgaca
    14401 agcacgaatt tcagctcact gggatgtttc ttgtaaagca atgaggtgat gatggcgttg
    14461 agtcccaccg atttcccctg cccggtagcg ccggccatga ggatgtgcgg catcttggtc
    14521 aggtcggcga caaagacctc gttctgaatg gtttttccaa tgccgaaagg cagttcatac
    14581 ctggaattgg tgaacttttc ggaactgaga atagatttca tcccgacgat ttcaggcttt
    14641 tgattgggaa cttcaatacc gatggtgccc ttgccgggaa tgggggcgat gatgcggatg
    14701 ccgagggcgg agaggcttaa ggctatatcg tcttcaagat ttttaatttt tgaaatacgc
    14761 accccggggg caggaatgat ttcatataaa gtgacagtcg gcccgatggt agctttgatt
    14821 ttatcaatct gaatcccgta gttgccaagg gtttctacaa tccggttctt gttgctttcg
    14881 agttccgcct tattcaccga gatgccgccg tcgttatgat tatcaagcaa atcgggggaa
    14941 ggcatgatga aacttcccag gtcgagggta ggatcatatt cagaatcaat cgggaaatgc
    15001 tgagcatcct gttccctgtc ctgttccggt tcgggcactt ttacaggttc agcagccgtt
    15061 tcgaccatga actcaatttc ggcgcgcggg ctgacccgcg gcgccggcat gggaacaggc
    15121 tcaaagccgt cgctgtcggt ttctttcagc acgccggatg ccggtatatc aattttgtca
    15181 tcctgatttt tgattgtatt cccgggggca gttcccgaaa ccaggatatc cgtttcgttt
    15241 tcctttactg catattcaac ggtattgaac cggtcggatt tttcatcggc ggctttccgc
    15301 atggcctcct cttcttcacc ggaatactgc gataaggggt tgatctcccg gatcctgttg
    15361 cggagctgat gcagtgagaa attataggcc agcgccagca cgctgataaa aacaaaaatc
    15421 agggctatgg cagttcccat ttcaccgaga aggccttcga gccactgggc cgttttgtat
    15481 cctgaaatac cgccaagaat tgacagcttc gaatgggtga aaataaaacc gatggtcacc
    15541 gaaagccaga ccatggccag gatgaaaatg cccaggcttt tccatatcgg gacaatggta
    15601 tgttttagcg aggcccgcac gccggcgaag aaaatccagg ctaccaggaa gaacgtgccg
    15661 atgccgaacc cctgttttac caggagcacc gataacgcag cgcccatcct tccgcccagg
    15721 ttgtttgccg tcagcgagtc atcgtgaagc acacgcgaga aattgatgcc cgagaagata
    15781 tcatctgtgc gcgaggtaaa ccaattgaca aggtatgatg cgaatgaaat acacaggaaa
    15841 acggaaaaca gcagtaaaaa ggcgccgatg atctgcttgg ccttttcgct gttattgtcg
    15901 cctcctgaag acttctcctt ttttgaagca ggtctcgaag tcttgccctt ggcttcttca
    15961 ggcgcggaac cggctttctt ttcgttgcgc agttcattct tcggacgttt aatttcgttt
    16021 ttctgttcca tccggatata aaagggacat aaccaatacg gctacatgta ttacccatgc
    16081 ctgccgggga taggcaaaag taacaaaaaa gctaatttcg tgagggttat tgacttaatt
    16141 tatagtaagt caatattgtc gttaaaaacg gcagccgccc ggcgttagtg tacgccccgg
    16201 aataatttat tccaacggat tttaccgctc aggagcgact tgtgcgaatc gagcgagagc
    16261 catacaaaaa cagcttttcc aacgatgtgg tcttcgggca cgaatcccca aaaacggctg
    16321 tcggccgaat tgtggcggtt atcgcccatc atccagtagt agttaagctt gaaagtataa
    16381 ctgttgcttt ccttcccgtt gatgaatatc ttgttgcctt ttacctcaag tttattgagc
    16441 tcatatgctt cgatgatgcg ctggtacaac ggcaggtttg aaatcgtaag gggcacggtt
    16501 acccctgcct tggggatata cagcggaccg aagttgtcga cgttccaggc ataagtggaa
    16561 tcaaacggga agatgtaagg ctgccagaat cccttcggct gaactacggg atgcacttct
    16621 ttcacattgg gaagcgcttt aattttttcg agcgactgtt ttgtaagcgt gaggacgtaa
    16681 tcgccctgtt cggtcatctc gatttcttcg gtgatgttga gtttatcctg tacgcgcttg
    16741 ctgatggggc ttccatcggt gactacgatg tacttgaatt gcgagttttc gggtatatcg
    16801 gcagcatggc cgttaatata taccaactga tcgcggattt ccagggtatc tcccgggatt
    16861 gccaggcagc gtttgatata attttcgcgc ttatccacag gtcttgaaac gattttacca
    16921 aaattgtagc tgtcattcca gaccctgtca tgaccgaatt cacgaaccag cccgtaatag
    16981 ctttgattct gcattttcag cgcaacagtg tcgccgtcag gataattaaa aactaccaca
    17041 tcgtttcgtt ttaccttgcc cagaccgggg aaacggtaat aaggccagtg tatgagttca
    17101 gaataggact tggcgtattt ggtgagcggc atggtgtggt gcacaaacgg gaaggcgata
    17161 ggcgtattcg gaagtttagg gccatagctc accttgctga caaaaaggaa atctcctacc
    17221 agcaacgatt tttccatgga agaggtgggg atggtatagg cttcgatcat gaaaatccgg
    17281 atgatcgaag ctgcaacgac agcaaaaata atggcgtcaa cccattcgcg ggtaaccgtt
    17341 ttctttggcc tgacacgttt atcgggctga atgtattttt ccttttcgga taagcccagg
    17401 taaggcaggt atgcataagg aaccagcaca gcaacagcct gttcccatag tttgtacttg
    17461 ccgtaacttt tcgccgtttc gaccagcatg agcatgatca taaacaggtt gataaacggc
    17521 accagcatga agattagcca ccaccagggt tttccgttga ttttattcca cactacgtaa
    17581 ttatagacag gaataagcga tttccatgct ttagtcccgg catcctgaaa aatccagaac
    17641 aggagagcgg gaaatacaag gatgaaaata attagagagg tcatcaggtc catgaacggg
    17701 aatttttatg ttaggtttag tggtatgcaa atatcagtga atatctcctt cgatgtgaca
    17761 ggttcgtagt taaaatgtgt taacagaata tttgaaagca agttaaatcc tgcaacgctg
    17821 catttttggc actcccggat accttttaat ttccggctat acggtattaa attcccagca
    17881 ggtccttcat gctgaaaatg ccttttttgc cggcaataaa ctcggcagcc agcagggctc
    17941 ccgctgcgag ccccttgcgg ctcttagctt catgtcggat tgtaatgaaa tcgttttccg
    18001 attcataggt aaccgtatgt gtgccgggaa cagtaccctg cctaaccgat tgaatgaaca
    18061 ggtcggaagg gttgtccgtc ttttcatttt tccacttcga ttttccgtca atattccgga
    18121 tgatatcttc ggcaagggtg atggccgtcc cgctgggggc gtccagtttt tgtgtgtgat
    18181 gaacctcttc gatgcttacc tcgtaattat cctgcgtgtt catgagccgt gccagccgcc
    18241 ggtttatctc gaagaaaatg ttgacgccga cgctgaaatt tgaagcatag agcagggcgc
    18301 cgttcttttc gatacagcga gcctttactt cgccggcctt ttcattccag cctgtagtgc
    18361 cgcataccac cggtatgccg gcatcaaaac atgcatacat gttggaaacg gccatggccg
    18421 ggatcgaaaa ttcgatagcc gcgtcgcatt ctttcagcag atgacctttg gatttccagt
    18481 cgtcggcatt gtcgattacg acatggattg tatgcttacg tgaaagagct gcttcctcaa
    18541 ccgcttttcc cattttgcca taaccgatca gagcgagttt catgattttc ggggtgttta
    18601 ataaaccggg aaaggtaaag gtatattatc agaaacgcaa agaaagcgtg atgccgcctg
    18661 ccggcctgat accggccacg ggcggaggcg tccatgggga catgtgcagt gaaaggtcat
    18721 ctgtaacatc gtaatcgaaa aaatgagcat ctacgactgc gtcgagcgcc tgaataatat
    18781 accatcctcc tgttatgatg taggatagtt caaggttgcg acggtaatat tcccggcctt
    18841 ccagcaactg gctttggtta taggtattta ccagattgtt cggcggcgta ccgctgcctc
    18901 ccgagagcac ataggaatat gcatcacgga actcatgata attgtctgtg ttaaacttca
    18961 cgaagtaata catggttcca aaaccggcat aaacaatggg cattttccag tatttgtgat
    19021 tgtatgcctg tccaaggcct ggcaggacaa gggcgaagat cgcagccttt ttgggagagt
    19081 gctttgagac aaaagcagaa tcagcggcgt gcttttgaac ttttctgatg gtatcggttt
    19141 cctgggcaaa tgaccggaac gggcagagga gaaccgtgaa tacggcaatc agtatccagc
    19201 ccgtaatgcg ccggcacctg accattaccg ggtaagctga tatcgtccgg gaggaatgat
    19261 tgttgctttc agaacaatca gaggccaagg attgaaatga tcttttcaag gtcggaatcg
    19321 gaagtaaagg ggatgacgat gcttcctttg ccttttgcat cgcgtttgag ctcagcctta
    19381 ttgctgaaac gttcctgaag ttgctgtttg acggcgttat atttttcggg caattccgtt
    19441 ttttcgggtt tcaccggcct ggcaggtttg ccgtctaagg tatcgaggtg tttgacaagt
    19501 ttttcaacat cgcgtaccga aagttcgtcc tggtttattt tcctggtgat ggcgatctgt
    19561 ttcagcggat cttccaccga gataatgctg cgggcgtgtc ccatggatat ctgcccgtcg
    19621 cggagggctg cctgtacctc ggcgggaagt ttaagcagcc gcatgtaatt gctgatggta
    19681 gtcctgtttt ttccgaccct tttgctgagt tcttcctggg taaggtcaca ctcatcaatg
    19741 aggcgggaat agctgatggc aatttcaatg gcattgaggt tttcgcgctg tatgttttcg
    19801 ataagggccc attcgagcat ctgttcatca ttggctaccc tgatgtatgc ggggatggtt
    19861 tcgagcccgg caagttttgc ggcgcgaagc cttcgttcac ccgaaataag ttggtactga
    19921 tcgaagccca ttttccggat ggtaagcggc tggatgatgc cttgctccct gatggactcc
    19981 atgagatcgg caagggcttc ctcatcaaat tccccgcgtg gctggaaggg attagcctcg
    20041 attttatcga tcggaatatt ggcaatggcg ccggcaacat aattacccga aatgtccttg
    20101 gaggtaatgt ctgtttcggg gctttcgagt attgcgctga gcccgcggcc aagggccttc
    20161 ttctttacag tgttcatact tattctacct ttatttcttt atcttcctgc cgcagcagcg
    20221 tgagttcatt cttctgaagt atttcccggg ccaggttcag gtaattgata gccccgatac
    20281 tgcttgcatc atgcatcagg attgattctc cgaaactcgg ggcttctccc agacgggtgt
    20341 tgcgataaat gatggtttca aagaccatgt tctggaaatg ggtttttact tcttccacaa
    20401 cctggttcga cagccgcgtg cgggaatcat acatggtaag gagtatacct tcaatatcca
    20461 gttggggatt gagccgcgac tgaacgatct taatggtgtt cagcagcttg ccaagccctt
    20521 ccagggcaaa atattcgcat tgtaccggaa cgatcaccga atcggcagca accagggcat
    20581 taacagtgac aagccccagc gacggggaac agtcgatgat gatgaaatcg tagctgtcgc
    20641 gcagcttgtc tatcacgagc ttcatcattt tctcgcggtt gggcaggttg atcatctcga
    20701 tttcggcccc cacaaggtca atatgggcag ggagcaggtc gaggttcggg gttgccgttt
    20761 tcaggataat gttgcgggga tccacctctt caacaataca ttcataaatg gatgttttaa
    20821 tgtttttggg atcaaatcct acccctgagg tggcattggc ttgcggatcg gcatcaataa
    20881 taagcgtctt gcgttcaaga accgccaggg atgcgcctaa attgatggca gtggtggttt
    20941 tacccacgcc tcctttttga tttgttatag tgattacctt acccatcgtg atgaaaatgc
    21001 gttgactcat taaggttatg aaatatatcg gcttcctgaa agcgggcagc ttgaatagta
    21061 ttattataac ctgactgtct tgctgttata tagcaatatc aatgtttttg atattaccga
    21121 tcatctgcaa aaatacaata ataccgacag gcgattatat cagtgttaat aagttttacg
    21181 attctcgcta ttaccggcat ataaaaatca acacactatg gcagtcaaag ctggacgcca
    21241 catatttagg acagacagga agcaatattg ctatttatcc aggtcttcga aatccacttc
    21301 agcgtggtta tggttatcgg catattgccg ctcgggcgga ataattcctg tctcgatgaa
    21361 ggaaatgaca tccttcagcc cgtcgccgaa tttttcaaaa tcctcacggt agaggaatag
    21421 tttatgcttc ttataaataa accgtccctg agcattgtca aactctcttt tactttccgt
    21481 gatcgtaatg taatgctgct ctgtacgcgt gcgttttacg tcaaaaaagt aggtgcgttt
    21541 tcctgcttta acagctcttg aaaatacatc ttccctgctt tccgagcgat cattttcttc
    21601 cattgtagtc agtttttatt tgttcttcct cttactgata gtgcaaattt atattttttt
    21661 taacgtaaaa aaattagaaa aaatatttga ggcatatttt ttttgtgaat tcttcatccg
    21721 ggcgtaaatt tttaatcggt tccgatagct taatttgatt atttttgctg ccctttaaaa
    21781 ccatgttcat ttacacgtta tgctaagcag aagatttctc aggatcaagg tgttgcaagc
    21841 tatctacgca tttatacagg atggtcctga aagtattaat tccggtgaaa agcagctcgt
    21901 ggttagcctc gataaacttt atgagcttta tatcaggcaa ttatcgctgc tggttgaaat
    21961 atccgacttt gcccgccgcc gtatcgaaga aaacaagctg aaatttctgc cttcgcggga
    22021 agatcttgat cccaatacac gctttattga taaccgcttt atcaaccagt tggctgctaa
    22081 cagggatttc ctcaagaaat gcgaatcctt taaaatttcc tgggccgacg aggaagagat
    22141 ggtccgtaaa ctttataacc agttgcgcga gagcaaggca tttgttgcgt atatggaagg
    22201 cccggcgcct tcttacaaaa aggataagga cctggcagag catattttcg taaatatctt
    22261 tgcagaatcg gaattgctcg agagttttta tgaggaacgc agcatatatg ggagcgatga
    22321 ttttgaaatt tcattcatca tgatccagaa gaccattaaa ggcttccggg ccgatgcgga
    22381 tgaatatacc tcattaccca ccctgcttaa agacgaaaat gataccgatg gcagcgaaga
    22441 tcttgatttc gtcaaaaaac tgtaccggaa aacgctgatc cacagcgatg aattcaacac
    22501 cataattgct gataaagccc agaactggga actcgaccgg attgccctca tggatactat
    22561 cctgctcaag atggccatga cggaatttgt tgattttccg tcgattccgg tgaaagtgac
    22621 gctgaatgag tatattgaac tggctaaaag ttttagtacg cccaagagta aaatttttat
    22681 taacgggata ctcgataaat tgatcgtgga attcaaagcc aacggcagac tggtaaaaac
    22741 cggccgcggc ctgatggaag attgatggac gggctgccgg cgctgcgctg aacgactgta
    22801 ctgcctgtaa aagaaaagcc aactttagta tcaggaattc cgccgaatca atttcgataa
    22861 atctcaaaca cattttacct gtaccgatgc ctgtccccga aaattgcgat agcaagaatt
    22921 ttactgtacg tgattacagg aattctgatt ttgagaatat tcaggcgctc tggataccat
    22981 tgggcctggg aagccgccag cggggcgata ccgctgaagt gattgagcaa acgctgaaag
    23041 ccggcggtaa actcctggtg cttgaagaaa cgctgtcagg aaagctggtc gggacatcct
    23101 ggatgacggt ggacggacgg cgtaactacc tgcatcattt cggaatcgca gaaacctatc
    23161 aaaacaaggg gctgggaaga atgctgctgg acgcgtccat tgcttttgcc agggaaaaag
    23221 gtctgcaact gaaaattgaa gtccaccgcc ggaaccagca tgccatcaac ctgtatacca
    23281 ggaacgggtt caaatatctg ggcgattacg acgtttatat aattcgcgat atttaaacac
    23341 cggagcaatc attcgctcct atttcataca tttgcaatca tttcactgct atgaaaccaa
    23401 aactgctgct aacagccctg atggctgtcc ttctgataac aggccagtcg tgcaataaaa
    23461 gaggtaaaaa tgatttaccg gccgatgtta tcatgaatcc gaacacggct tccgggaaag
    23521 ttgatcccaa cgcattgccg gtcattgagt ttgaaaccga tttccatgat ttcggcaaac
    23581 ttatgtcggg cgaaaaagta acctattcct ttaaatttaa aaataagggg aaaggtatgc
    23641 tgctcatctc aaaagtgagc acatcatgcg gatgtactat ttccgaatac cccaaagaac
    23701 cgttgaaatc aggggcggaa ggtactatcg acgtgagttt cgacagtgaa ggaaggatcg
    23761 gtttacagaa taaaaccatc acggttttca gtaataccca gccttccact acgatgctgc
    23821 gtatccaggc cagtgttgtt gacgtaaagg atatgtagca gttaattctg aacctgctta
    23881 cttatggggt tctgtcatga acgcctgatc aataaatgca aacaaaataa aacagataca
    23941 atggaacaat tgaccatgtt attaatggct ccggccggcg gcgcccagaa gggcggaagc
    24001 ggattaagct cactcatttt ccttttagtc atcttcgtcg tgttttacct tttctttatc
    24061 cgtccccagg taaaacgtac caaggaacag aaaaagtacc gtgagaacct gcagaaaggg
    24121 cagaaaatca tcaccatcgg cggaattcac ggaaggatag ttgaaatgca ggatactacc
    24181 gttaccattg aggtggaagg cggcacgagg ctgcgtattg aaaaatcggc agtagctgcc
    24241 gacgcccagg accagcttgg caccgaagcc aaatagaaat cagccgtttc gcagaagcat
    24301 aattattcag gcttcgaata gccaattggg atgatgatgg aaaatgccga caggcagacg
    24361 gatacaaccg aaaacatagt taccgttcaa ccgaaaaaat catttcgcaa aaaattcggt
    24421 attttcgtgg tttgccttgg catatcttcc ctgatgtggc ttttcattga gttgacccgg
    24481 gattatacca ccgacctgaa atatgcggtc tccttcacca atgttccaaa ggatttaatc
    24541 ctgcagaatc agaatgattc gagcattacg gtcggcatca cggcgcaggg gtttgaactt
    24601 ctgaccacac ggcttttcag gaaacggcat gagttaaccg ttgacttgtc cgaaatccgt
    24661 atccgcaagc aaagccaggg ttattcggct tatgtccctg cttccaagct ccttcagcag
    24721 gtggcacgac agatcggttt ctcgaaaacg gtttcctata ttcgtcccga taccctgttt
    24781 ttcatgtttt cggatgttta ccgtaaggaa gtacccgtac ggctgaattt ctcctattcc
    24841 tttgaacaac aattccagtt gtacgattca atagaatggt cgccgaagac agtgatcgtc
    24901 tcaagtataa aagatgtaat cgacacgata cgcttcgttt caaccgtccg gacggtgaaa
    24961 acagatattg acagcagcct gagttttaaa attccgttaa gccggcctct gggtagggga
    25021 atgatctctt actccacaga ttcaatccgg gtccggctgc cggtgcaaaa attcacggaa
    25081 actactttta ccatcccggt gaggattgtc ggaaatacat cgcagttaaa gatatttccc
    25141 gatcaggccg aaattaaatg catggttccc atggtggatt accgtaacat tgaagcggat
    25201 aatttcctgg cgacggtgca ggccgatgga aaaacgctga ctcagaatcc gaaactcagg
    25261 atcaacctgg tgaatgtgcc attgggtgta aaagtgataa aggtaaagcc cgaatttgtt
    25321 gaatacatca tcatttcgcc atgatcaggg tagggctcac gggcggaatc ggaagcggca
    25381 aatcggtggt tgctgaaatt ttccggcatc tcggagtgcc ggtgtatcat gccgacgcgg
    25441 aagcaaaaaa actatacgac ctgcctgatg tagttcagga aatcagccga gaattcggca
    25501 gcggggttat ggactccgga ggcacagtgg atttcaaagc cctggcttcc atcgtgttcc
    25561 ccgatccgct gaaacttcag aagttagatt cgatcatcca tccgctggtg gaagctggtt
    25621 ttatatcctg gttggcggat taccgggatg cgccctacac ggtcatggaa gcggccatcc
    25681 tgttcgaaag cggtttcgac catttatttg ataaaaccat catggtattg gctcctgttg
    25741 aactgtgcat tcgccgggtc atggcgcgtg attctgtgac cagggaacag gtcgggcagc
    25801 gtatgaaaaa ccagtgggat ccggaattaa aggctgaacg cgccgattac gtcctggtga
    25861 atgacgaacg gcaactgttg ctgccccatg tgatcgcttt gcatcacacg ctgtcgggcc
    25921 ttgaaacgaa agcctgaaac ggattttcat ggtgtttttc cggaaagcct gcaggatcaa
    25981 aggagtttat acgatataag acgatgcccc ggtatttttt tgccggggtt tcttattttt
    26041 gtaaaagaaa gtaatagtcc gtaaaaacaa aacgatatgt cagaacagat tcttggagta
    26101 ggttcacggg tgaggcaccc gcaattcgga aaaggggtgg tggttcaggt gcgctccgat
    26161 gcatatgaaa tcgggtttat tgaaaccggc gtcaggcaga tactgcgcga ttttgaaggg
    26221 atggaagtca ttgaagccgt ggaaacgcct tccgatcttg ccacttacga aaaaattgaa
    26281 aaaagccttg tgcggattct ccgccgcttc agcgatattt cggaggtaac ccagatgggg
    26341 caaagatgga acaacggcag gatggtgctt tacccgggcg atgcctccac aaaagagaaa
    26401 gaggttccgc ttgatgcatt ttttcataag atcgtaatgg tccgcgaccg gctgcgcgtg
    26461 ctcgaagcaa agatcaatgc cagcggtctc agcgatgaag ataaagttga actgcagcaa
    26521 tatataaccc gttgttacgg aagccttacc acgttcaatg tgctcttcag gaacaaggaa
    26581 gaccagtttg tgggggagaa aacggcgtga caaacgggca taactaaaat tcatgcgcac
    26641 gcatgctaca agagcgcgag catgagtgat gtgattttat taagtttgtg gtaataaagg
    26701 ggaaaaccgg gttaaagcga ggattgaaac gcacgcgcta caagcgcgcg agcaagggta
    26761 aaaaaatcat tttccagtca agctaaatta atatgcggat gaaaactaac aactattttc
    26821 aactaattgg gatcgttttt tttttcagtg tgatccatgg gtgcgatcgt tttgatacta
    26881 gagtaaaaat gattaataca tcaaaccatt tagtatatta tacatggatg tctgatagtt
    26941 cttataatct attatttaaa aggataaatg ctcagatgga atctgccaga gaggattcaa
    27001 ttatcttgaa ccgttacttc atatctgtca gacctaagat agatacaatt gaggaagcaa
    27061 tcccaggtac ttgggaagaa tactttgcaa atccaaatag aaaaatttgg gttttcatta
    27121 ttcccgattc gttaatgagt caacatgttg gggaaataac aagtattcag taccaaatta
    27181 aaaggaagtt ctttgataaa acctatctgc tagaacataa tggtataatt gacttatctg
    27241 attgatgttt tgcttaaaaa taatcaattg ccaggttagg gaaggtaatt ctaaaaagga
    27301 acttttagta gtaatataaa gctttgaata tcagcaatta aagttgataa ccatgcccgt
    27361 gctcgcgcgc gcttgtagcg cgtgcgcagc acagctctct cgtgacgtta acaatcaaat
    27421 tgaatttttg tagcttatta gagcgcgttt gattatattt gtcttatatc cgtttcatca
    27481 taagggtttt atgttatata tgaaaaatgt ctgacagata taagatattc aaaggtgatg
    27541 agccatattt tgtcacattc acgattacca actggatcaa agtccttgag gatgattcat
    27601 tcaaaatggt attgattgag tcaatcagat actgccagga acataaaggt ttgcaggtat
    27661 acggatactg tattatgcca acccatgttc atatgatgat tcaggctaca ggcgataatt
    27721 ccgtttccag tattttacgt gatttgaaaa cacatacagc taaagcgatt gtcaggaaac
    27781 ttaagaatga aaaacctgat ggttatcagg aaatattatc ggtatttgca aaagcaggca
    27841 gggatttgaa acggattcag aattacaaag tctggcagga tggaaatatg gcaaagatgt
    27901 tatatagtaa ccggtttatg cttgaaaaac tgaattatat tcataacaat cccgtaaaat
    27961 atggtctgtg cgaagagcca tgggattatg tattcagttc tgcaactaat tattcaggga
    28021 aaacatcttt gattgatgtt attctgttaa gtttgtggta ataaagggga aaaccaggtt
    28081 aaagcgagga tggaaacgca cgcgctgcaa gctcgcgagc agaggcaagc acgtttaaac
    28141 gctgttcgaa aaacattttt agatcatatg ataaaagatt tagcaccaaa cattacacgt
    28201 aagagacttc tactggaagg attttataca atagatgctg ataaagcaac gataaaggat
    28261 tattttgatt acataaccaa agagttgaat gtaaggacat acagcgaccc aatcattttt
    28321 gaaactggaa accgagggaa aaaggaaaac caggggtttg atgcttttgt tccattaatt
    28381 gactctggga tctcttgcta tgtttgggta tatatgaaat ttatttctgt gattatttat
    28441 acttgtaagg attttgatga gaaaaaagca gtggattcaa caaaggcttt cttcaagata
    28501 ggcaaggtag agtggaatat attttaattg cgactttgca gagaattgtt gattttcaaa
    28561 ataaaaccaa atgatttcaa taattctctc aatagtgatt ccggataata tttatggata
    28621 gcctgtataa tctgatagat aatgccagcc gtcaataatc aggaatatga gcggtctgca
    28681 atgcttgtcg ctaaacggga atccagcgat tattcatgaa gggggaaaat cccttcccga
    28741 actgtgaccc tccgaccaat aaccctttcc tcctgtgaac cagaatgcgc aatttgcagt
    28801 caaaaaaatt tagcctgtca gcagaagcag ccctaaaaaa caaagcctgc catcaatgtg
    28861 aaatttgatg gcaggcttgt tatataaaag gatcagaaac tggctttcag ggagaaaacg
    28921 aagttcctcc cggctcctga aattcccgag ctgtacgggc ggtaccgctg gtcggtgatg
    28981 ttttcgatgc cggcgcttac cgaaagccgg tcggtaaacg ggtacatggc tttaaagtta
    29041 agggtgtacc acgcggggga atagttgttc ccgtcggcgt ctttggcata tatttcatct
    29101 ttcgattgtt cttcctgggg caggtcttca aatttgcgtt ccccctggta aacggcgtat
    29161 atctccatgc tcagtttacc ggtgttgtaa ctcaggcggc tgattccgta taagggggcc
    29221 gcatgacgca acgggctggt agtgccgtca tcgagttctt cctccccgac ctggtaattc
    29281 aagtcggatg aaatgctgaa accggcgggc agcttaactt ccaatccagc ctggatacca
    29341 tatacatagg ccgacgcggc attctgaatg gcttgcaccc ggctcagttc gccctcgtaa
    29401 agaatgctgt cgagtccgtt gagctggaaa ttccggcgta ccatcgcatt ctgcaaaatg
    29461 gtataaaagc cggtgacatc cagttttata acatcgtcaa aaactttggc gattccgagg
    29521 tcgaaattgt aagcgtattc tgctttcagg tccgggttgg gaacggtaac ggctcccggt
    29581 tccgagtcaa atactttgcc caggtcatcc acattgggcg agcggaaggc ggttccgaaa
    29641 tttgcgctga taacccaggt ttcatcggga cgatataccc cgccgatgct tccggtaagc
    29701 gcgccttcgt tgatttcggc tgttgtaaac gggaaaggat agaaagtagt gtcgaagtcg
    29761 gcattcagga tatactggtt ataacgaaga ccggcctgca gggtgcattt ttccgaaacc
    29821 ctgaattcat cgttggcata tacggccagg gaactccagt ccgactgcgg gtagcgcgaa
    29881 gggcccgttt cgctggtgcc cgtcgaaata tccgtcagca ttccggtaga ggtaacatcg
    29941 ttataaacaa attcagctcc gtagagcagg gtattcttcg cattgagcga tttgttgaaa
    30001 tcgaggttta ctgaccaggc ttccaccttt tcggtctggg tgtttcgttc atccttgttc
    30061 agcgaccggt caatacggct ttcttcaaag tattgctggg cgatacggat gctcatcccg
    30121 tcataaacgg catttttccc ggcatgggta atgttcaggt tgttcattat ccataattgc
    30181 gggccataat tccattcggc ataccgggct gttccgtttt taacgcggtt atgcctgtcg
    30241 taacggccgt aggatgacgt ttcggaaaaa tggaatccgt actggaagtc ccacttctcg
    30301 ttgggcttaa agcggacttt ctgcatcatg ttgatctggg agtaagcggt gggaatctgc
    30361 agcctcgggt cgtcctgggt gatcaccttg tccacaccgt cctgacgctg aacataatat
    30421 ggtttcaggt attcgtcagg gccgttgctt ccctggcgca ggttgtcaaa gtcccaggaa
    30481 ctgatgctgg ttaccagcgc ccattttttc catccgacat taacgtcgaa atgagctgtt
    30541 ttttcattat tggccgatga atagcgggtg acggcctttc cggtgaccag cggtttatcg
    30601 gtgagggaaa gctggggcgt taacgtctga aagctcatca ctccgccgat agcatcgctg
    30661 ccgtaaatta ccgatcccgg tccgaaaagc acttccgtat tttcggtggc aaagggatcg
    30721 aggttgatga cgttctggat gtttcccgac ctgaagatgg ccgtgttcat cctaaccccg
    30781 tccacagtat agagcagcct gttggtggca aaacctctga tcatcgggct gccgccgcct
    30841 tgctggcttt tctgaatgaa aacctcaccg gaagcgccca acaggtcggc agcagtttgc
    30901 gggttcagca atgtcagttc tttggccggg atggagatga tcctggaagg gacattgcct
    30961 gatttttgtc tccaccgtgt cgccgagatc acaacctcat ccaggttcag attattgatg
    31021 gccagcaaaa cttcataatt tcctgatttg agtccctggt agctggttat cactgtttta
    31081 taaccgagcg accggatttc gatttttgcg gcatcacgga aggccgagat atcggcctgg
    31141 ccttctgcat ttgtgatcgc ggatacgttg ggcgcttcgc tgatcagggt caccagaggt
    31201 atgggctgtt ttgtttcttt ttctctgatg gtcactatct gggaaaaggc aatgcctgaa
    31261 atcccaataa acaaccatgt caatagtata tttttcatta ttgcattcat ttaaaggtaa
    31321 gtgaataagc gcctgaaaac aggtgcggca agcccggcgg ctttttggcc ggcaggtgca
    31381 ttccattcgt tgatgtatgt ttaacttacc cggggaggag gcgtcggcgg cggaaatgaa
    31441 aataaaaaaa gccggtttga atgaatggta aaccgttttg ttgcaatact ttgaagtgca
    31501 gtatgccagc cggaattttc agaaaaatat agggatttga aataggaact aatctgcttt
    31561 tgttgttccc tgttccgggg atcggtgtgc agtatcttac ccgtcaacag ggatagctgc
    31621 ctgttaagct tcgattcttc gtcgtcgcac cagcaccagt aaacgattcc attggcggtt
    31681 gtatcggtat ccaccacgtc gtacatccga ccgttgtatt cgaattctcc ggcatgttcc
    31741 cacctgagtt tatcgcgcga ttcgttcctg gcgaatttta acaggaccag ttgatctttg
    31801 tctatcccgg ctataatctg cttttttacc tctttcctta ccgcgtattt tttgtatttc
    31861 agccatgcat aagttgtaat tattggcgat accaggcaaa agatcaggaa aataccggca
    31921 attctgcttt tcattggtca cggtcagggc tggaatataa agcgcttcat tttaactgat
    31981 gcttgccggg ctgcgatata aagccgggca acggacgaca gccctatgcg taaacaaaag
    32041 taaaaatttt attgaatatt agcattcccg tttctgcttt agagcgggaa ggtgaggcca
    32101 tgcctgaggt atttcaggaa atacaacccg ggccgggcat ttgtaatcct gaaatcctgt
    32161 gctccggtaa tatctcagcc tgatctgtca agctgtcagg attgcagaac atgaagaaga
    32221 caatacaata aataccgcag gtcttactat atttgtttcc ggttagccac agccatacga
    32281 tttactgagg gtaaaacatg cctgtggtcg gtgtaaaggc tctaccggcg gttgccagat
    32341 gaagccggtg catcggaacc gatggaacag gttcgtggta ttcatttaaa acaagtttaa
    32401 aatggaaaaa atcaccaggt tcttcataac ggcaatggta ctgtccgtca tgatgcttgt
    32461 cacatcctgc atcgattgca tcgaaggcga tggcaatatg atcagcaaaa cgcttgatgt
    32521 ccccgctttc agcaagatca agctgaacgg cgatgcaaac attacactga ccagtgacag
    32581 cacggcgctg ttcctgatca aaggcgaaaa gaacatcctg gatgaatacc gggtggaagt
    32641 ggtgaataaa acgctggtta tcaaatcgga acattgcctt aggagccatg agcaggttac
    32701 gctgcagatt ccgtacaccg aactggaatc cgttgttatc aacggttcgg gatcggtcta
    32761 tgccaatgaa accgtaagat cggagtcgct ggatttaaaa atcaacggtt ccggagactt
    32821 tgaactcccg gtggaggtac aggacctgac gggatcgatc agcggctcag gcgatctgct
    32881 tctcaaaggt tcggcgaaaa acctggaata taccatcaat ggttcgggca atatcgagac
    32941 gccggatctc acatctgaac aatgttccgt gacggtgaac ggttcgggcg attgcagcgt
    33001 atgggctacc ggaaacctta aagtaaaggt gcatggcagc ggagatgttt actataaagg
    33061 aaatcctgca atacaaaccg atataaaagg ttcaggatcg gtaactaaga aagaatagcc
    33121 ctgtttggaa aatcattttc aggacaaata ataaacgcgt cgcgggatgc gttatagatg
    33181 tcgtaactaa aatcgcatcc cgggtatctc actaatattc cattttgtga aagctgtatt
    33241 ccaaacttta tccatcgcct taatcgtatt tcttgcttcg aacggttatg cccagacggg
    33301 cggaaataat gcttacgaat tcctgaacct gtccaattcg gcccgtattg cggcgatggg
    33361 cgggaacttt ctggccgttg acgataacga tatcaccctt acccaggcca atccatcgtt
    33421 gatcaatgcc tcgatgaata aaagcctggc gcttggtttc gttgattatt acagcgatat
    33481 caattacggc tttgccatgt actgccgtga ctttaaaaaa gtagggaacc tggcgggaag
    33541 ccttcagtat atcagttacg gcgatttcaa gctgaccaac gagcagggcg aagttcaggg
    33601 gaatttcaat gcaggagagt acgcctttaa cgttggctgg ggccgtacgc tggatacggt
    33661 tttcaggatt ggcgccaacg ccaaaatgat actttcgaac cttgaaacat ataattcttt
    33721 gggcgttgcc gtggatgtcg ccggcagtta tgttcccgac aacaatacct gcgtttcgct
    33781 acttttcagg aatatcggca ggcagatcac cgcttattcg tcaggcgacg ttgagccgtt
    33841 gccgttcgaa atccagttgg gcatgtcgca ccgcctggag catatgcctt tcagggtgtc
    33901 gttgctgctg actcaccttg aaaaatggga tttgacctat gatgaacccg acaatcagac
    33961 ttcgtctgta aacgaatcct catcccgttc cgacgtcggc gaatttgccg ataaggcatt
    34021 caggcatgtt gtgataggcg gcgaattcat gccggcaaaa tttctgagta tacggcttgg
    34081 ctataattac cagcgccgcc aggaaatgac catcgcctca aagaccggaa tggttggctt
    34141 ttcctgggga ctcggtataa gggtttcgaa atttggcatc aactattccc ggtcgcaata
    34201 ccacctctcc ggatcgccga attacatcac catcaccaca aatctcggtg attgggcgaa
    34261 gaagtaaccg attgccggct ccttattttt cgctattttc gcctcataaa agccagttta
    34321 tgaaagtgac catcgatccg gatgccgggt tctgctttgg tgtttcgcgg gctatcgagg
    34381 ctgccgaaaa gggattatcg gcaggggagg agttgtattg ccttggcgat atagtccaca
    34441 attccgggga actggaacgg ctgaaatcga aaggcctgaa aattatttcc catgaagacc
    34501 tgcccgtttt agaggggaaa aaagtgctga tccgtgccca cggcgagccg cccgaaactt
    34561 ttgaaacggc caggcgctat aacatcaccc tgattgatgc aacctgcccc atcgtcagca
    34621 aattacaaca acgcatcagg aaaagccatg aagaaggatt gaaaacaggc ggcactgtcg
    34681 ttatttacgg caaacccggc catgcggagg tagtagggct cgtcgggcaa accggaggcc
    34741 gggcggttgt cgtcaggaaa cccgaagacc ttaatggcat catcctttca agccctgttc
    34801 atctctattc acaaaccacc atggataagg aaggcctgga tgcgatggcg gcagagatcg
    34861 aaaaacgcct cagggaaaaa gggaatgccg gtctgttcgt tcataatacc atctgcggac
    34921 aggtatcagg acgtgcgccc ctggtcagga aatttgcaaa acagcatgat gttgtaattt
    34981 tcgtcagcgg gaaaaagagc tcgaatggag cctggctgtt ttcgctgtgc ctgtcggaaa
    35041 accctcgctg ccatttcgtt tcatcgaaag aggatatccg gaaagaatgg tttaatgccg
    35101 gcgattcggt cgggataacc ggcgctacct ccacgcctcc ctggctgatg gaggaaatag
    35161 cgcgcgaaat atcaacattt tcgtaaaatt ggacgaatag gattaaattt gcctgaacag
    35221 aacatcggga attttatcta tttaagttgc tcaccagtgt accttaggcg tctccagatc
    35281 accaacttca aaaattacgc tgaagccgat ctttcctttt cggaaaagat aaactgcttt
    35341 gtcggcaaca atggcgaagg caagaccaac ctgctcgatg ccatttatta tctttcgtat
    35401 accaagagct atttcgggaa tgtagattct cagaatatcc gccatggcga ggatttcttt
    35461 gccgtgcacg gcacctttgc ccgcaacggc aatacgccgg acgtggtaag ctgcatccag
    35521 aagtcaggcc agaaaaagca gttccgcctt aataaaaagg aatacgaccg cctttcggac
    35581 catatcgggc tgtttccctg cgtgatgatc tcgccttatg atcgcgacct gatcaacgag
    35641 ggcagcgata tacggaggaa gttcatcgac atggtgatct cgcaattcga caagccttat
    35701 cttgccgacc tgatccgtta caaccgtctg ctggagcaac gcaatgcgct gctgaggcaa
    35761 tttgccgaat tgcaccatgt tgaccgggag atgatctcac tgctggatga acagatggtg
    35821 gcgccggcaa cggctgtttt tgaaaaacgc agaagtttta tcgccgattt cctgcccatt
    35881 ttcagccgtt atttcgggtt tataagcaac aaccgggaag aggtcgggat caggtacgag
    35941 tcgcaacttc aacaggccgg ttacatcaat atggtgaatg gttgttccga aagggatctc
    36001 gtgatgaagt attcaactac gggtattcac aaggacgacc taatgttcga gatcgggggc
    36061 tttccggtga agcggttcgg ctcgcaggga cagcaaaaat cgtttgtggt ggcgctgcgg
    36121 cttgcccagt acgattttac aaaagcccgc atcgggtata agccggtgct gctgttcgac
    36181 gatattttcg ataaactcga cccgtcgcgg gttgagcagc tcatcaagct ggttggcgat
    36241 gaaaatttcg ggcaggtttt tgtgacggat accgaacccg accgtatccg ccgcatcttc
    36301 agcctccttg aagcggaaca taaaattttc aatatcggcg cgggcgctgc cgtggaaatg
    36361 agttcataaa tgatcaggaa atcgacggaa atattgctcg ggaaagccat cgaggaatgg
    36421 ctgaggacga atcattttga tgaaaagctt aatgaaacga ggctgctttc ctcctgggaa
    36481 aaggtcgcgg gaaagctgat agcgcggcat accgaaaatc tttttatcaa agaaagagaa
    36541 ttgtttgtaa aagtggattc ggcagccctg aagcacgaac ttacctatat gaaatcgaag
    36601 ctgctgaaaa agctgaataa aacggcaggg gcggaggtta ttgaggatat caggtttatt
    36661 taaagggata aaggtgatcg gcattaccag gcggatggac gagtaaatga ttgaagaaag
    36721 agtggcacct cacccccggc ccctctcctt aaggagaggg ggggctcctg aagagaggat
    36781 accgcaccac cttcctgcaa aacttttaaa cggaatgttg ttttgtcgat cacccatgaa
    36841 taataatgca catgaacatt aaaaattttg aatttaaggc cagtgttgac aatattgaaa
    36901 aatatgaaaa tttactgctg aagctgaatc caaggtttca ggggactgac catcagaccg
    36961 acacctattt taacaccggc aaaggaagac tgaaactgag ggaaggaaat attgaaaatt
    37021 ccctcgtgga ttataaccgg gaaaaccttc aggatagtaa aaaatcagag gttatccttt
    37081 acaggcatgc gccttccgat tcgctgaaaa gtatcctggt acatcaattt ggaatcctgg
    37141 tggttgttaa aaagataaga aaaatctact ttatcggcaa tgtgaagttt cattttgatg
    37201 aagttgaaaa cctgggttct tttattgagg ttgaagccat cgatgatcag aataaattca
    37261 gtgatgagga tttgaaagaa caatgtgatt actattaccg gtattttggc attcagaaag
    37321 agcagcttca aaaattgtcg tacagtgata tgttattgaa ttttgaaaat aaagaataaa
    37381 aaatatttgc ttctggtaac cgtttttcac ttgatctcca cggccagcat tctgtcccct
    37441 tccaggaacc cctccagccg gtcgccgatt tgaaccggcc cgacgcccgc cggcgtcccg
    37501 gtgaaaatgt agtcgccggt ccgcagcgtc acaaactttg aaacatggga gatgatccgg
    37561 ccaaaggaaa agatcatatc ctttgaattt ccttcctgaa ctaccttccc gttgagctga
    37621 agactgaagt tgacagaatt tgcatcacgg aattccgata ccggcacgaa ccggcttacc
    37681 ggcgccgaaa aatcgaacga tttggctctt tcccagggca gccctttttc catggcttcc
    37741 cgctggagat cgcgggcggt aaaatcgata ccgatcccaa cggcatcata ataggtgccg
    37801 gcaaattttt ccgcgatatg ccggccgagt ttacagatac gaagtaccag ctcgcattcg
    37861 taatgaagtt cggaagtgaa ttcaggatag tagaaaggcc ggttgcggat aagcagcgcc
    37921 gtatcaggct tcataaagaa caggggctcg gcgggaatgt cattttccat ttcctccaca
    37981 tgggcaggat agttgcggcc gatacaaatg atcttcatag tatgctattc ttcaatcatc
    38041 aacctttgtt aaaattcctg agtttgatgg ccgtcaccac tttcttggtg gtgagcggga
    38101 aagcgctttt catgatccag tcgtaataag aaggctcttt ggcgaatatt tcctccacct
    38161 tcgcgccttt gtatttgccg aagttaaaca cttcttcgcc cttgtcgttg aagatcacgt
    38221 tacccatcaa gtcggcgctg cggctgtaat aggagaactc gtgtaacgct ttgatgtcgt
    38281 tcacaaccgg tttcgagatg ttgccgttcc tgtcctcgta ttcaacgcct tcgtaacgat
    38341 cgagctgggc cagcaggact tcataagtgg cgcgggtgtc ggcttcggca gtatgggcgt
    38401 tttcaagcgg cttgccgcaa tagaacttgt atgcggcttt cagcgtgcgc ggttccattt
    38461 tatggaaaat gttctgaaca tctacgacgc gccggttttt catatcaaac tctagtccgg
    38521 cccgcaggaa ttcctcaacc agcaggggaa catcgaattt gatgttgtta taccctgcca
    38581 ggtcgctgtt gccgatatag gcgatcagat ttccggccag ttctttgaag gaaggcttgt
    38641 ctttaacatc ttcatcttta atgccgtgta tggcggtgac ttcggcggga atgggcattc
    38701 cgggattcac aaggtttaca cggatttcct catgaccatc gggattgatc ttaaggatgg
    38761 ctatctccac gatacggtcg ttggcaatgt tggtgccggt ggtttcaagg tcgaaaatgg
    38821 ccaggggacg ggtaagcttt aattgcatgc tgaaattcag gttgttaaat aattatggta
    38881 tatcttggaa gcccgataaa tcggtgttca aagtaacgac aattctcccg gaattttatc
    38941 ctgaaccggt attttaaact aatgtctgat atgaatcgga cgctcggccg ctctgaccgg
    39001 aaaaagcatc aggtttaatt atcaatgaca tgccatcttc aaaattaatt gattggataa
    39061 acaaaagcga ctgaaatatg cttccggtgc atccgaagtt cctgtaaccc aaatgaatgt
    39121 taataggtaa gaaatataca gtattattaa tctgataaat caataatatt tttactttta
    39181 gcccaatgtt tgttaagaag atatttcgtt attggattat cttgccggca tgcctcatcc
    39241 aactgtcttc agcttgtcaa actgtgaagg aacgaagtga taaggcttat gccggtctgt
    39301 ccggttcatc gggaaccaca agggtcaggc tcttgaatga gttggtttca tataggctaa
    39361 ccgattccgc cgataaagct gccaggcttg ccaggatagc tgtttcggaa gccggtgaaa
    39421 taaaagacaa ctccttgttg gccaggtcat gggtagcgtt gggttctgta tatctgacaa
    39481 aaaaaatgcc tgattcggca aaatatgcgc tttaccaggc cggccggtta ttcacgaagt
    39541 attccatgac tgaagggaaa gatttctatt atctgacccg gggacgcctt ctgctgatgc
    39601 aaaacaggga agtggatgcg attgccgaat tccgcgatgc ggttcccctg gcggagaaac
    39661 gatccgacta tgtcacgctg ggcctcattt ataagcaact ggccgcttgt tacaaacaac
    39721 tcaacaggcc aaaagaactt atcagcagcc tggataaggc ttccttttat ttcaggaaag
    39781 ggggcgatcc gttcattctg ggcccccagc agatggggct cggcattctt tacctggaca
    39841 tggggctgga agaaaaagcc agcgagcagt tcatcatggc tgaaaaagcc agcgagactg
    39901 cttccgacag catttccctt ggctatctct actccaatat cgccggtatt tattactcgc
    39961 atgcctgccg tcagaaagct gaagaatact acgagaattc catgaagata ttccgggcaa
    40021 ccaggcatta ccagggaatg gcatattgcc tgaaccagat ggggatgggg tattttaatg
    40081 aaaatcagta taaaaaagct ttgccgttat tgtcgcgggc agctatgctt aagaatgagt
    40141 cctccgactg gcagggagca tgctttgtat atagtaacct tgttgaagtt tatatcaggt
    40201 taaataagcg gaatgaagcg acatcggcca ttaaaacagc ttctgaaatg tgtatcaggg
    40261 cgggcgataa gttatccatg gcagcattca tgcaggcaaa cggttcctat cacacctatc
    40321 tcagggaata tgacaaggct ctcggatatt accagcgcag cattcagctt gccagggaac
    40381 tgaatgtaca gggcatcgtg atggaagacc ttcgggcaat ttcggaaatg taccagttga
    40441 aaggcgatgc tggcggggcg ctggaatact ataagaaata cacgacaata aaagattcgc
    40501 tggccaaagc atccgatttt aaagctctgg ccgatctgcg gatgaaatac gaaacagaaa
    40561 agaaagaccg gcagttgaag acccttctta ccaggctgaa atacgggaag gagcgatatg
    40621 ttttcctggt aaacggaata atcattgcgg ttctcctgat catcttcacg atattgtttt
    40681 tccggatgag gggaaattat tccggctcta agcaacgcct tccggctttg cggaagcaga
    40741 caacagccgg tccgcggatt gaggcaacac cggcccctct gatgcttccg gctgaaaaag
    40801 cttcgaaaaa ggtgctgacc ggagaaatgc agtcggtact gtggatcagg cttcaggaac
    40861 ttatggaaag ggagaaactc tatctgcgca acgaccttac gctgtccgaa ttagcccgga
    40921 aactgaatac aaataccacc tatctgtcga aggttatcaa tgatatcaca cagcagaatt
    40981 tcagcaatta cctcaataat ttccgtattg atgaagcctg caggctcctc tccgggcctc
    41041 acggacgcaa cctgaccatc gaaggcattg cacagtcggt tggatttaat tcaaaaagcg
    41101 cctttaatgc ggcttttaaa aagatcaaat atgtcacgcc gtccgagtat ttatccaggg
    41161 aacaaaacgc agccgtccgg cagtcactgg aaaagaatcc tgttgcctga ttcacaggcg
    41221 tttgcatcct gtcctgattt ataaatccgg acagcgctca ttgtaatata tcagggagca
    41281 gcgatatatt tgctctgccg tcaggagtgt aggccggctg cagataagat gggcgcgggc
    41341 ttcaggggat gccgcctgcc caaaaatctt tatcaaccgg agtacaaaaa cgacgaaatg
    41401 aaacaggtat aataccggcg tgacggtgta caatccaatg caattggaat ttcccgggcg
    41461 cccaacggct aataaccctg taacaggtaa agaattaaaa cttcataatt aaccaaaagc
    41521 ctctttttat gttggcaatt acgggcatta tttttatcct cttcaacctg actggttcta
    41581 tttctgttac cggaaccaaa gccggatggc tttttcagaa gaaggagtat gctgctacag
    41641 ttactgcttt ttcaatttac cggaaagcct ggcctgtttc gcataacaga tttgcctttc
    41701 aggtggcgga cgtctcgggt aaaaagatga ttccggatag ctttattatc tgattttaaa
    41761 tgcgggcagg cttatgagcg atttttcctg ataagcctgc cttgttttca actgaacttc
    41821 gttccgaaaa tacccgttaa accctccgac tgcttcaaaa ataaccttca cttaaccgat
    41881 acaaacacct aaccttatga gacttatcag aactctgacc ggattattct tgcttgctgt
    41941 tctcccgtat gctgccccgg cggccgggca aactgctaat acggatacag gccctagggc
    42001 ttattttaaa ctggatggta acggaattga cgccgtttcg gggcttaagg cctccattct
    42061 gcatggaaaa gtaatcaggg ggcatgacgg taaagaacag ggcgcctatg aatttacgat
    42121 caacgacagg gattatagta aatcaacccg cagcctgact ttcccggtcg acatcaatgc
    42181 ctcaaaatgg ccctcactta ccataacggc atgggtaaag gcatcgaatt attttatgcc
    42241 catgtatgtg attaccaatt ataccaataa taaaacaagg ggacttattc tggataagga
    42301 tgatgatatg taccgctggg ggatgtgctg cggcaaagac gggatgcttt ggggaccaac
    42361 cctggtggag ggctggacgt tcgtagccat gatctatgat gataaaaacc aggaagcccg
    42421 cctgattgtt aacgacgagg tgttctcgtc gcgtgcaaag atgcacagcg gagagaataa
    42481 agcttttgtc ggggctttta aaggggcaat cgatgatgtc cgtttctacg atcgtatcct
    42541 gtccgcggct gagatcgagg ctatttcggg caagccgatc gttaaaggcg ccgacgacct
    42601 cgccataaag gaccggtatt cctacaagga aaggctgcaa caggaaaaag aaaacaaact
    42661 ggtgatcggc ggggtttata ttgtggataa agaagagatc aaagtcttta caacaaccaa
    42721 ccaggaaaac acggccgatg tgttgaggaa tcccgacagc ttaaaggtat tggaaaaact
    42781 tcccgacgga tggtataaaa taagttatgg caatggcaaa accggttttg cccgccggtc
    42841 ggaaattgtg aacaaggcgt accctgaagg cagcagcgtg ttaaaattcc ggtttatgaa
    42901 ctggcttagc aatgttttcg atttcaccaa actgaaatca tggatttttg ttctgatatg
    42961 cgccgtcatc ctcttttatg tcaaacgtaa gttcaatacc cttgatgacc gcttgcttaa
    43021 gttgcggccg gctcatgacc agtttgccga tggcggttca aaaagcgatg cctcggttcc
    43081 tccgtctcag agtttccttc acagaatata tcctgtaaaa aagtaccggt ggtatccgtt
    43141 actgggcggt atcctgcttg gcgcgaccat tttcatcgct tcgttctggg atggaaatga
    43201 aatggaatgg tttttcaatg aaggtttcaa catcctcccg atagggtacg acaggccggt
    43261 ccattggttt atgtatggca tgtgcatgat cacgatactt ctgacactaa gctggatcgt
    43321 ggaaagcttc gtgatcggcg gaccgttgat cggtctattg agggtcttta ttcttctgat
    43381 actgaattgt atggcgctgc tggtttcgtt cttcctgctt gttctgattg ccttcctggt
    43441 gatcggtata ctggtgctga aagtcctggg gagcgcagcc ggttcaggaa aatacaaatg
    43501 cccccattgc gggcgtacct tctcagccag cgcaggaagc aatacaagat gtccgggctg
    43561 tggtgccagc cttacaacgt agtggctctg cgttcatcct gacctcctgc ctggttttta
    43621 attcaaatca aacaatgcta tgttaatcga tcgctattat ttttcccgtg ctttccccaa
    43681 caaagagata gacaaatgtt gggaggaagg caataccata atctctgccg cttatggcaa
    43741 cggacaatgg gtgcttatcg ccgccagcga aagcgacctg gacggacaac agtggaatac
    43801 ccagggtgat tttccgtccg gcccgatcaa cgaagggtgg aatgcccgta aggatattaa
    43861 ctggctatcg tacggcaacg gcgcctgggt cgtttttatg tcgggcaaca ccggctattc
    43921 cgaccaggtt tggcgtacca gcagccgttt ccctgagaaa gaaatcagtc agggactgaa
    43981 agacgggtac atggttaccg gactggctta cggcagcgac cgctgggcgg ttgtgctgac
    44041 caagggctcg ggattcaccg cgcagcaata tgatagttac cggtcgtttc ccaaagatgc
    44101 cattcagaaa ggctgggata acgggttcga tatcacttcg ctggcctttg gcgacgggaa
    44161 atggggattg gtgatgtcgc aggggacagg cttcggtgcg caggaatgga tcacaggttc
    44221 cgaattcccg gtaaacgaca tcaatgccaa gttgcgggaa ggatacagca tcacgcacat
    44281 cgcttatggt gctggtttgt gggccgttat tgtgtcagaa accgaaacag aggaggatga
    44341 tgaggaggaa gaggaaaccg acgaaaccga tacggaagat gccgtttccg acctggatcc
    44401 ggaagctgaa cggctgcatg acctggggat agattttctg gaaaaagaga attacgacaa
    44461 ggcgatagtg aatctgaaaa aggccgtcga tattgagccg gagtatgccg atgcgctggc
    44521 agggcttgca acggcctata cctacaagga tgatctggat actgccatca cctattatga
    44581 gaaggctttt gccctggata atgaaacgcc cgtcctggtt tcgaacctga tactcacata
    44641 caacaacaaa ggatgtgacg ataaaattct ggatgtggtt aaacgcgcag atcctgaatg
    44701 ctttgataag attgattttg cggaaacact gaatatcatc ggcgtttgct atgccgatgc
    44761 cggcgatttt aacaatgccg tgaaatttta caacaaagcg ctgaaactgg cccccggcaa
    44821 caaacagata cgcgataacc tggacgaatc ggaggaaagc ctccggcaac ctgccgctga
    44881 ggaagtttct gttccgcaac ctgccgttca catggttcct gatgtggata acgatcagct
    44941 cctcgaaatg gccatgaaag aacttcatgc catgacaggc ctggagacca taaaacgtga
    45001 tgtagatgac ctgctgaagt atatcaggat tgaaaagctc cgccgcgagc gggggcttga
    45061 cgccaatccc acggttctgc actctgtatt ttcgggccct ccgggcaccg ggaaaacaac
    45121 ggtagcccgt ctgttgggca aaattttcaa agcattggga ctcctttcca aaggccatgt
    45181 ggtggaagtg gaccgttcgg gactggtcgg ggagtttatt ggccagacgg cccagaaagc
    45241 aaacaaggtc atcgattcgg cgctggatgg catcttgttc atcgacgagg catacagcct
    45301 ggcgcctgaa gacgatccgc gcgatttcgg gcgcgaagcc atcgaaacct tgctgaaacg
    45361 tatggaggac agccgcgacc ggatgatcgt gatcgtggga ggatataccg aagagatgaa
    45421 acgctttatc gacgccaacc ccggacttca gtcaaggttc acgagatact tttatttcga
    45481 tgattacaaa ccggaacaac tgagcgaaat tttctcggaa atatgcgttt cccggaagtt
    45541 tacaatggat gacgatgcca gggccaaact gctccggtat acagaatttc tgtataaaag
    45601 ccggacgaag aacttcggca atgcccgcac catgcgcaac cttttcgaag aggttatccg
    45661 cctgcagtcg gggcgcctgg caatgctgag ccagatcagc gataacgatc tttgtgccat
    45721 caccgaagcc gatatcaccg aagctgtcaa ggatgaattt gccgacgaga aacaggaaac
    45781 gattgccgat gtgctgaaag atctcgaaga attggtaggc ctcgaaaatg taaagaagga
    45841 tatcaatacc ctgatcaact acctgcgtat tgaaaaatta aggcaggaaa aaggaatggt
    45901 agctaatccg gtaagcctgc acacggtgtt cttcggccct ccgggtaccg gcaaaactac
    45961 ggtggcaaga ttgctgggaa gaatatttaa ggtcatgggg ctgatatccc ggggacatgt
    46021 ggtggaggtt tcgcgggccg atcttgtggg gcagtatgtc gggcagacgg cgccccgtac
    46081 catgaaagtg atcgattcgg ccttacatgg aatcctgttt atcgacgagg cttatatgct
    46141 gacaccggcc aacggcggca acgatttcgg acaggaagcc gtcgataccc tcctgaaaag
    46201 gatggaagat gaccgtgacc gactcattgt cgtggttgcc gggtacacca acgaaatgaa
    46261 ccatttcctc gaatcgaacc ccggtatgaa atcacgcttt aacaggcact tttatttcaa
    46321 cgattacagc cctgaggaac tcacggatat cttctgcaag atgtgcgaaa gcaggcattt
    46381 tatcctggaa gagggcgtga agggaatagc gctggaatat atgcagcaat gctgggatga
    46441 acgggataaa accttcggta atggccgcat ggtgcgcaat ttctttgaaa aactggtgca
    46501 ggcccattcc gacaggcttt caaccatcgc cgaccttgac gaaaacagcc tggtgacttt
    46561 tacgcaagcc gatatcagaa gagtggtcgg acttggggga gcggaaaagt cgggttcaaa
    46621 atcgatagga ttcaagaata atagttttaa gtcatgataa agtatctcga aaatcgtgta
    46681 tggtcgctga atgccaaaat cgtgaacacg gaagccgatc ctgcagagat caaccgtttg
    46741 ctggcggaga agttgacgtc tggaatattt gccggcgagg ggatccggtt ttcctattcc
    46801 gaaaatccta tcggaaacta ttcgttcagg ccggtcgagg tgacgcttga aatgaccgca
    46861 aaagcgatcg gacaatatca gcagtttctc gatgaaaagg ggatatcgtt ccggatctca
    46921 tcattccgca tgatcatcaa tgcatttggc ttcctgaata ttattccggt ctttgaatat
    46981 gcctgcaagc cgggcaacaa cagctatgcg gagatagaga aaaacgggga tgcttcggct
    47041 gaaatcattg acgccatttt cagcgatatt tctgctgcta ttgacgtcct cgacagcgcc
    47101 ggaattatcc ggaaatcatc ctattatcat tttggcgtgc cttccggact tgaaaaatcc
    47161 ggaatccata cccgggagga cagctataac tacctcgttc atatttttta cttcggcgaa
    47221 aaggaattgc tcgacgacac ggttaagcaa ttcaattcgg aagagaatgt ccttacgttt
    47281 gaggaacact gcctcttttc cgaatttccg atatatttct gggaaatgaa gcacgatgtt
    47341 gccgacaacg aactggttga tctgatatcc atcaacagtt acatgctgtg cgaaacggtg
    47401 gcggtaaaca atgcgctgca tgtgtacaac tcgttcctcg atattttgaa tcaaaacctt
    47461 gaggtagatt ccaaccacct gcgcgagatc tttaattaca acacctggct cattcagaat
    47521 ctcaggctgt ttaacccgaa ttttaccctg taccagtcta aatttatgaa gatgtaccgg
    47581 caaaattccg atatctcacc caaatatgac cttctgaagg atgccgaaca atcgctcacc
    47641 tttgccatcg aaggcatcga agtcacgcgt acgcagcagt cggagcgcat tatgcaattc
    47701 attttggcgt tgtttacggc tttaacgctg tactcggtga tcaccgacgc ttatgcgctg
    47761 ataacgagtg aagtgcaggc tgtgcctttc tccatgcatt ccacgcagac gatcatcttc
    47821 atcctcgaaa cgacggtagt gctggcattc atcatctttt tcaggaaaat cagcagaagg
    47881 atatagacgt tacgataaga ccgtcatagc cggagtccgg ctaacgatta accggtgcaa
    47941 tgcttccggt tgaattcgct gaaatccgga gttttcgtgg ctctaacatc cggttttcgg
    48001 actcaggcgc cggtagcgaa atattccgtg cgatcgggtt cggttcaagc cagtttcctg
    48061 attttcccgt tatgtcacct tttttcctgt ttccggcgtt tttatcgaat tggttttacc
    48121 tttgtggcta ccttcaaagg aatgttataa ttaaatgaaa ttaagatgag attttcagga
    48181 atgcgcagca tgttgctctg gtccggcctg gcctttttat cgctggtatt tggatcccgg
    48241 caggccgggg cccaggttac accggaaaaa tcgtacagcg attatgtgaa cccgtttata
    48301 ggaacgcagg gggaagggaa cacttatccg gctgcggtag cccctttcgg catgatacag
    48361 attggccccg ataccgatat cgaacgctgg gaaaccgctt ccggctacga gtattccgat
    48421 tcattgcttt acggcttcag cctgacccac ttcagcggca ccggtattcc cgattttggc
    48481 gatttccttt tcgtgccttc cacgggaaaa atcaagactt acgggggaaa acgcgatgcc
    48541 tggaacgccg gttcgtaccg gacgcgcttc agccacagcc aagagtccgc tgcgccgggc
    48601 tattattctg tccgcttgcc ggaatatgac atcaacgccg aaatgacggc ttcggacagg
    48661 gcgggcttcc tgcgttttac ttttccgcag accgacagcg ccaatatcat gatcgacctc
    48721 aaacatgtgc ttcagtggaa ggtggtctgg tcgcaggtac gcgtcatgga cgactccacc
    48781 atcacgggat accattatgt gaaaggctgg gcacgggacc ggaaagttta ttttgccgcc
    48841 cgcttctcga aaaaatttga aagattcggc atttaccgca acggacgtgt ggtggaatac
    48901 gacccgttta agacctaccg gttcgtcagc agcaacgaag cagccgacac caacctgcaa
    48961 ttctttgccg cctataaaac tgccgccggt gaagcgatca cggtaaaggt agccgtttcg
    49021 ggcgttagct ccgacaatgc gttgctcaac atgaataccg aaataccgca ctgggacttc
    49081 agccgcgtta aggaagaaac cagggacaaa tggagccggg aactgaagag gatagaggtg
    49141 gaaggtaccg acgacgaaaa aaccactttt tactcttcga tgtaccacct gttcctcaca
    49201 ccgttccttt atcaggacgc cgacggaagc tacctcggca tggataaaaa tatccataag
    49261 gcgcagggct ttaccaacta caccgtgttt tcgctctggg atacttaccg ggcggttcat
    49321 cctttgttca tgctgataca aaacgaacgg aatgcggata tgatcaattc tatgctgaag
    49381 cattacgagc aaagcgccga ccatttgttg ccggtgtggt cgttttacgg caacgaaacc
    49441 tggtgcatga tcgggtatca tgccgtgcct gtgattgccg acgccgtagt gaaagggctt
    49501 aaagggattg atgccgcaaa gtgctatgat gctgtgaagg ctacagccat gaacagggaa
    49561 tatgataacg tgatgcttta cgcgaagctg ggctacctgc cttttgatgt ggagaacgaa
    49621 tcggtttcaa aaacgctgga atacggttac gacgattact gtgtagcccg aatggccaga
    49681 tacctgggta aaaccgatga ttacaactat ttcatgaaca ggtcgaaagc atacaggaat
    49741 cttttcgatc agcaaacggg ctacatgcgc ggaaaggatt cgaaagggaa ccggaggacg
    49801 ccattcagcc cggatgatta cagcgatgat ctgcgggatc gcgatttcac ggaagggaca
    49861 agccgtcagt attcctggta tgtgccccac gatgtgcagg gattggccgg gcttatggga
    49921 aaagagaagt tcgaagccag gctcgacacg ctgtttgccc tcaacgtacc tgaaaacaac
    49981 cagaccgccg aagatgccac cggccgtatc ggcgaatact ggcacggcaa cgagccaagc
    50041 catcatatca tctttcttta tgattatatc ggcaaaccct ggaaagcgca gaagctggtt
    50101 cacaaagtcg taagtaccca atacggcagc aagcccaact ctctatgcgg caacgacgat
    50161 tgcggccaga tgtcggcatg gtatattttc aatgccctcg gcttctatcc cgtttgccct
    50221 tccggcagtt attatgctgt ggggagcccc tgtctgccga aagcggtagt aaacctttcc
    50281 agcggcggaa agcttactat cagggccaac aacctctcga aggataacat ttacatacgg
    50341 tcgatgacgc tgaacggcaa agactggaac aagacgtata ttccgtacga agagatcagg
    50401 aacggcggtg agatcgtatt caacatgacg aacaagccca accggaaatg gggaaccgcc
    50461 cccggcagcc tgccgccgga agagtaaaaa gaaagtgcag gatgggaccg gcgatacccg
    50521 gaggccgctt gatgatgttt aaaagtagaa atacattcat gtttttataa acagagggat
    50581 atgtgttttt cagcaacagc aagctttacg gcaggggcgg ttataacggc tatcggggtt
    50641 gcctctgaac tgaaagtcag aaagaaatcg cagcgactct ttgccgctgt tcccctgatc
    50701 tttggccttc agcagatagc cgaaggattc gtctgggtgt cgctgcaaag gccggaccaa
    50761 ctcctcatgc agtacatcag tacctatgtc ttccttatca cggccgatgt actctggccg
    50821 gttatggtcg cggcctcatg tttactgatg gaagagaatc ccgggaaacg gaaaatcctt
    50881 aaaatcctgc ttgcggcagg cgcagccctt tcactgtatt atgctttctg ccttctttcc
    50941 tttgccgtaa cgcccgaaat attgaactgc cacatcaact atgccggtga atttccgcat
    51001 gtgctggtga taccggcatt cctggtttat ctggttgtca ccataacgcc atttttcgtt
    51061 tcaggcgtaa aggggatgcc ctggcttgga gccgttttgt tcctggcctg tgtggtttcc
    51121 gtgatttttt acatacagaa tttaacctcg gtgtggtgct tttttgcagc cattctcagt
    51181 atctttattt acctgctgat aagtcgcgaa aagcaaaaag gatcctgatc ccggactgga
    51241 atttaccctg ttactgcatt tcccggcgta tgatccggta tttttacgac gcaggataat
    51301 ttggcaattt attgctatat tcgtgcattg agaaggtgcc ggctgtatac ttgttagcgt
    51361 tcgtgcttga agacgttaca aaattcagat attcagacat tcatccagtt atgaaatatt
    51421 tatttaccat actaaccgtt ttagtggttc agacagtttt tgccgtgaaa ccatgcgaca
    51481 aatgtgatct ggaaaaagtc agaattgtaa atcaacatct cgacagtttg acttaccaga
    51541 tgttaaccga atttttgtgc acctttgaca actcctgcaa aaacaatgtc gaatattcag
    51601 agttcagtaa cgagactctt ttctcggttc ttgagaaagc tccgaatttg ttttttcagg
    51661 ttatggcaac aagacagata gataataaaa tcattctcaa agaaattgaa aacccaataa
    51721 acgacttaat caaccttcaa acggtttacg acaagacaaa aattgccacg actaagacag
    51781 aattgaagac taaatatctg aatgcgttaa ttttggcggc taataaaagt ggactgaaac
    51841 taaaaaaata aagcacgaaa tgcaaaaaaa cgctatagca aatgcgggtt tacgtgttcg
    51901 ttgaaatatt aaactattaa tacacattta ggcaggcaga cagttgaaca acaatttaat
    51961 cccgcacttg ccatagggcc ggccgttatg cccaaccaca acaactttga gttctatgaa
    52021 aaaatcagat atttatgaga tagcgcttaa gattttgggt gtctatttgt ttattcacat
    52081 tgtagctctg ctaagaacca ttttttcttt cctgagttat tatgatatag ccaggtattc
    52141 tccggctcaa atagataagt atagtccgat gccggaattg atggcatcgg tgattcagtt
    52201 ggttattgat gtcactctgg cttatttgct gacattcagg acaaaaagaa tgactcatct
    52261 gatatgttct cagcaagatt atacagaaac tgtccaactc attactgagc ctaaatacct
    52321 ttaccagatc gcaatggtat tggtcggatt atcagccata gtccggactt ttcctgattt
    52381 tctcataaaa cttcagcatc acattcataa ggttcaatta gatgatcact cttatcttta
    52441 tgacatcaat tatcttttaa ccgaagcgat agagttattg cttggtttcc tccttatcta
    52501 ttacaaccgt cccatttctg aaattctcac aaaggataaa aagaaaagag tagctgaata
    52561 aaaaggccgt acctgcacgc attccggctt ttcctcgtgc ttcttctgaa aactaatgct
    52621 taattttgac gcctttactt cccgttgagg taatcggata aaagccggtt ggcagacagc
    52681 ggcggaacac cggcaatcat ctgatagcaa gcaggatgta acatccgatc atcgactcgt
    52741 aaactctcat tccatgaaat acacatcact gtttctttta ctgtttttgt ttttcgggtt
    52801 gaagcccgtc aaagccaacg aaccggagca tcagagcatt tatcagctca tcgaaaggat
    52861 actgccgggc aaatcatccc aatttgtcat tggagacctg aaaactgccg gcaacaccga
    52921 agcctttgag ttaagcggga aaaatgggaa aatcatcatc aacggcaccg gcgaacttgc
    52981 cattgcaaga ggttttaact ggtatctgaa ccattattgc catacccgcg tttcgtggta
    53041 taaggatgag ccggttaacg ctcctgccgt attgcccgcg gttgatacaa ccgttatgca
    53101 ggagtgccgg ttcgaaaagc gtttcttcct gaactattgc accttcggct acaccatgct
    53161 gtggtggcaa tgggacgatt gggaaaggtt catcgactgg atggccttaa acggcattaa
    53221 catgccgctg gctatcaccg gtcaggaagc ggtgtggatg gaagtatgga aaagctttgg
    53281 gatgtcggag gatcagatca gggcctattt caccggtccc gcccaccttc cgtggcaccg
    53341 gatgggcaac ctggatggtt tcctgggacc cctgccgcag aactatatcg accatcagac
    53401 ggaactgcag aaaaaaatac tgaaacgcga acgctccttt ggcatgacgc cggtgctgcc
    53461 cgcgtttgcc ggacatgtgc ccaaagccat caaggaaaaa ttcccggatg caaaaatcac
    53521 ctcgatgggt tcctatggta ccggtgaagc gtatgatgcc ttcttcctgg acccgatgga
    53581 tccgctgttc atcaaaatcc agcagaaatt tctgaagatc cagaccgaat atttcggtac
    53641 cgaccattat tatggcgccg atccgttcaa tgaaatggac cctcccgata ccaccccgca
    53701 atatttgggt tcggtttcaa aatccattta cgaagggatg cacaacatcg acccggaagc
    53761 cacctgggtt cagatgggct ggactttcta ttacatggat ctgtggaaaa gtgatccggc
    53821 ctgtttgaaa gccatgatca cggcggtgcc tgaaaacagg atgatcatcc tggattattt
    53881 tacggagcag gaagaggtat ggagaaacac aaaggcctgg cacggagcgc catatatctg
    53941 gtgctatctg gggaatttcg gcggcaatac cgaaatggcg gcgcctctca aggaaattgc
    54001 cggtcgactt cctaaaaccg aaaacgacac aacccacgga aaactctacg gcatcggttc
    54061 aaccctggaa ggattcaacg tgaaccgatt tatgtttgaa tggcttttcg agtatgcctg
    54121 ggacaaaaac gtcaccaatg ttgatcaatg gataaccggt tacgcaaaaa caaggactca
    54181 gaacaatgat cccgtgtccg aagaagcata ccggaaattg atcggtctgg tttacaataa
    54241 gcagatttcc aacatcggga taggaagcct gatgcagacg cgtccgctgc ttaccggatt
    54301 caaaggctat caaaggccca acaagtataa ctatcgggaa ttgaccgata tcttgaccct
    54361 gatgctttcg gccaatgaaa aaagtctgca gagcgcagcc tatcaaaagg atatggtcgt
    54421 ggtgacgaaa cagatactgg ataacctcat catcccgctc cggaagaaga taaatgcggc
    54481 ttatgtgaac aaagacacgg tggagctgga caagcaagcc aaaatctttc tcggggtcat
    54541 ggacgaccag gaccggctgc tggcaacgca acctgagttt ctgttgggga agtggatcca
    54601 cgatgcccgg caattcggga acgacgcggc aagcaaagcc tattatgaaa aagatgcccg
    54661 tgtgttaata accacctggg gcaacgaagg gaacgagatc attgattatg ccagccgtga
    54721 cctttccgga ttaatagcta cctattacaa ggtgagatgg gaaatgttct tcgaaaagct
    54781 aaagatgaat atcaggaata acggcacttt ggatatggag gcggtaaacc gggaaatggc
    54841 atcctttgaa tggaactgga ccagtcagca acagccgttc atcagtgtgc cgcaaggaaa
    54901 cgcgctcgaa atggtgcgcg aaatccataa aaagtacagt tctcttttca gcatctgcaa
    54961 tgattgattg accggcataa agtaaaa
//
LOCUS       DAOPYV010000011        54756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_448_length_54756_cov_12.510137, whole genome shotgun sequence.
ACCESSION   DAOPYV010000011 DAOPYV010000000
VERSION     DAOPYV010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 54756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54756
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_448_length_54756_cov_12.510137"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2610)
                     /locus_tag="PKI35_02455"
     CDS             complement(<1..2610)
                     /locus_tag="PKI35_02455"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNX43270.1"
                     /translation="MILNNVNLKRLPLLVIMILALNVNGQVSKENSSVANRIPPPDSP
                     FTSAYRAGNNSDAPAGSISVAENATYNAYSAEQLVQNVLVTGCLTASNVRFGYYNRNN
                     NNTWVNHTWSGTPGNRQLAYFSKGTSSFPLEEGLLLCTGNASSAMGPNNNTGKSDKKV
                     SNASDPDLATITGRTMYDAAVLEFDFVPAGDSIEFTFVFTSEEYLEYVGSQYNDAFGF
                     FLSGPGISGPYTNNAVNLATIPGTTTPVSINTIHPAVTNNYGTFPATNETYYFSNASG
                     SLTMQYDGGTVVLKATYAVQPCSTYRIRMSIADASDQEWDAGVFLGAKSFNSQNISLT
                     NYALNNYGSFNEGQNNIFEGCNDYLRIERSGSDISAPLTVNLNISGTATNGTDILTSD
                     NQPFPTQITFPANVAYIDIPYSAVSDGISDNDETFIVKAPVSCPCESNQIYVTQTIHL
                     YELANITSVSALNLQCFGQSNGTITVNVSGGSGSYQYSINNGSTWQNINTFTGLSAGT
                     YTILVRDPGSCYPNVTATATIGSPTAIVAQAGQDVSICNGESTQLNGTGGVLYSWSPS
                     TGLNYTNIANPIATPSVTTTYTLTVTNASGNCSSTDQVVITVNPVPEFTTSVTDVTCY
                     AGNNGSIAVTPGSGAISDFQYSKDDGATYQNSNIFNGLVAGTYFIKVKNISGGCESDH
                     EYVIINEPSVLTAGESHTAIACNGGNSTVTISATGGAAPYTGTGDFTQAAGTHTYTVT
                     DANGCSDDVTVTISQPEILVAGESHTAIACNGGNSTVTISATGGAAPYTGTGDFTQAA
                     GTHTYTVTDANGCSDDVTVTISQPEILVAGESHTAIACNGGNSTVTISATGGAAPYTG
                     TGDFTQAAGTHTYT"
     gene            3634..4434
                     /locus_tag="PKI35_02460"
     CDS             3634..4434
                     /locus_tag="PKI35_02460"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX43271.1"
                     /translation="MKAGITTLLIIFVLISRARGQEQPEHNVRVDGFVTTIRGNALQY
                     VNIINLNKRTGTITDAKGSFRIAASPDDTLVFSSVGFQNKKMRVQPYPGNPVCPVHIY
                     LQEDTITLSGVTIHDWPRNLTALRQAVLAQKIEEPAIADLNLNVVVPDAPQPGSPITL
                     PGMGNPGLTYTIPGPITALYNAFSRRGKSMQRYAELTRQDRIHEMIARKYNPEIVKDI
                     TGFKSDEEIIAFMDYCNLSVSFLLNATEYEILCSVKDCLANYLAEKQQ"
     gene            complement(4446..5426)
                     /locus_tag="PKI35_02465"
     CDS             complement(4446..5426)
                     /locus_tag="PKI35_02465"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HNX43272.1"
                     /translation="MKRHPKNPIITRDDIPPIPPELVDATSAFNPGAIKDGNIYRLML
                     RVQGRSRETFLVMAQSPDGVNFSVENRIVHFKGIENVKGRIFHTYDARITNLEGRFYI
                     MFAMDMEDGCQLGLGVTDDFRDFDFLGLTSNEDIRNGVLFPEKVNGKYLRMDRPNKAR
                     HTGGPTSGSTIWLSGSDNLIDWTPVAPLIDGRFHYWDEFIGSGPPPVKTRKGWLHVYH
                     GVAGHFGSANIYQGGVMLLDLKDPSKVLGRCRCNILEPREIWELAGQVPNVTFPSGMI
                     VEAYDREGFAIESSEVKIYYGAADTSVGLLISTVKELLDASMEGDIPCFP"
     gene            complement(5447..7825)
                     /locus_tag="PKI35_02470"
     CDS             complement(5447..7825)
                     /locus_tag="PKI35_02470"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43273.1"
                     /translation="MNYGHFIKNGKEYLITNPRTPSPWINYIYNGRYFSTISNNGGGI
                     SYYKSPLHGRITRYRINEVPPDRPGKYIYIRDNETGEIWSLTWQPVGKHAEAYKVAHG
                     FGYTRAEASANDIDSSVLFFVPEDDDQEIWKASLKNTGRKSRKLSVYGYVELALGHAM
                     VDLINQCDDQHFNRSHFEKDLNALFSTKTYWVTETSGTQQQENKEWDQWTFFTVNRPV
                     TGFETLRERFLGLYRNENNPESIEKENLSCQDTDFGNVVNALKVDIELNPGETTDLVF
                     SLGVIEKIRFGEMKEQRVQKYKSVAEVDAAFAKLNRTWDKYFSYTTVDTPDDDVNIFM
                     RYWLPYQAKVAFDVGRVISFYYWGIGRGFGFRDTSQDTIAVTISNPGKAKERIQLLSR
                     QMRKDGKVYHHFHGDGQGEFTCHCDDPLWFMLAVTEYLKETGDFDLLHDKQPFIDADP
                     DTILDHMFSVVNYAKNNLGKHGLPIFGRGDWNDTLDYIGGDEGGESVWGGMFYAAMLN
                     LFIELLGRLRMTTAQSEVAAVRENLVKAVEENCWDGEWYIRAFGEKDRRVGSKDNKYG
                     KIFLNTQIWPVIAGFPNRDRLVTAMDSVKKYLDSPEGPKKCTPAWREIDPKIGLVTRC
                     VWGKKENGAVFCHPTTWVIQAETMLGRSNQAFEYFKKMLPNRIDSETFVAEPYVYSQY
                     ITSNEHSQPGRASHSWQTGSAAWMYRVSYDYILGIRSTYDGLIIDPAIPSHWKSFKAE
                     RVYRQTRYIFEIENPNGSEKGVSRVFVDGNAIEGNLIPVSSAETCRVKVIMH"
     gene            complement(7836..9761)
                     /locus_tag="PKI35_02475"
     CDS             complement(7836..9761)
                     /locus_tag="PKI35_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43274.1"
                     /translation="MNLTWIDWSIIIVLFSIMLGMVFLSKSLLKSVTDFLATGRTGGR
                     YIISMFHGTAALGAITVVGALEQNYNAGFNSRWWEMPTSVILVAISVTGWVVYRFRQT
                     RALTMAQFFEMRYSRTFRIFAGMLAFLSGIVNMIIFPSVTAKFFIYFCGIPEIGLIGP
                     YSGTFILTTAVMVLVPLYFIFTGGHIAVMFTDFIQGVFVNIVFVIIVILLLVQVGWVD
                     IGEAVKAAPTGKSLINPFDASEVQDFNPWFFFIGMIGIIYTKLSWQGNSSFSIAAKNA
                     HEAKMADVLTNWRLVPQWGLFLMIVPIVAYTIFHHENYKVIADSINNMLAGFAPADQS
                     QLRVPMILNYMLPVGLKGAFAAIMLAAAITCHNTYMHSWGSIFIQDVVVPIRQKPFEP
                     KEHLKYLKLSILGVGITIFILSILFQQTEKVFLFLNISGAIFVGGSGAVIIGGLYWKK
                     GTTPAAWAAMITGATASSVNILLNQIITDFPINGQWGWLIAMVSASLVYVIVSLLTPG
                     KAYNLDKLLHRGEYAVQTELSSSDTEVIKGWKVLAPTKEFTKGDKAIYYVTTGWTAAW
                     VVVFIIGTLFYFFVKPFTNNQWMNFWKVYTYVFLTASIIVTIWFTIGGIKNLKEMIHA
                     LRTNIRDDHDDGFVNKH"
     gene            complement(9814..11652)
                     /locus_tag="PKI35_02480"
     CDS             complement(9814..11652)
                     /locus_tag="PKI35_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNX43275.1"
                     /translation="MKLFLRFVITCISLIPFAVPAQNPSHTFTAEGMDFKLDGKPLQI
                     ISGEMHYQRIPREYWQNRLQTAHAMGVNTISVYCFWNDHEPMPGKFDFKTGNRDLATF
                     IRMAQAEGLWVILRPGPYACAEWEFGGYPWWLLREKDMVVRSKDPRFLAAAEKYVMEI
                     GNQVRDLQVTNGGPIIMAQVENEYGSYGNDKEYMSLIRDMFVKAGFTVPLFTADGPVQ
                     CKDGYLPGILPAINGETDPDALMDTVNKYNNGSGPYFIPEYYPGWLDHWGEPKSDVNS
                     DDVVKDIKTLLNSGFSFNFYMLHGGTNFGFMNGANYNNEFPIQPDITSYDYDAPVNEA
                     GWPTEKYYAIKKLIFPFLPEGTLIPEVPERLPVISIPYFRLSEVHSLTSLLPKPIRSA
                     SVLTMEEVNQGYGYILYRTTIAKPGKSMLKINGLRDFAVVMINGIPVGTLDRRLKQDS
                     IEIQTRTANNKLEILVENMGRINYGRRMTDNRKGITGNVILGKQELKNWEIFPLPMTK
                     LPDFAAARTEKKKPAGPMYRRGTFTLDKTGDTFIDMSLWGKGVVFINGNNLGRYWNIG
                     PQQTLYCPATFLKKGVNEMFVLEMLDPEINLSRGLAEPVLDKLKPK"
     gene            complement(11674..12729)
                     /locus_tag="PKI35_02485"
     CDS             complement(11674..12729)
                     /locus_tag="PKI35_02485"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HNX43276.1"
                     /translation="MKINSLHICNLPTGEEVFRYTLENDHGIVIRLTNYGGSLTEAWI
                     PDKNGKTDDVLLCYDAVEKLIMNTEPYFGCIVGRTCNRIAGSAFKIDGTEYKVSPNKG
                     TFQLHGGFEGFNKKAWKSKAVSTDDDVSVILEYFSPDGEEGFPGNLQVKVVYSLNNNN
                     ELSVSYYAMTDKPTPVNMTNHSYWNLRGEGSGDILDHQLVILADQITEVDGNIIPTGF
                     LKPVAGTAFDFTTPHTIGERIGELYKGYDDNYCLRNLSGKLAPAARVFHPGSGRVMEV
                     FTTEPGMQLYTANWFDGSITGKGGVPYMPHAAFCTETQHYPNSMNIPGFPNVILRPGN
                     MYSSETRWKFSVADSIQ"
     gene            13039..15171
                     /locus_tag="PKI35_02490"
     CDS             13039..15171
                     /locus_tag="PKI35_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNX43277.1"
                     /translation="MNPKISPEDRVNDLVGKLTLEEKILQMQHDAPAIPRLGIPEYNW
                     WNECLHGVARNGIATVFPQAIGMAATWDPGLIHREADIIATEARAKYAEAIGKGEHGI
                     YQGLTFWSPNINIFRDPRWGRGQETYGEDPRLTTEIGKAFVTGLQGDDPRYYKVISTA
                     KHFAVHSGPESERHRFDAWPSMRDLYETYLPAFEALVKEAKVYSVMSCYNRVYATPTS
                     GNTFLLKDILRDTWGFKGYIVSDCWAVSDFYNFHNFVPSADKASALAVRAGCDLACGN
                     EYVHLNEAIKSGYLTEADLDVALRRVFMARMKLGMFDPPALVPYASIPPSANNTEDHR
                     QLARQVACESVVLLKNEHNLLPFSKKIRTVAVIGPYANDTSVLLGNYNGTPSAPVTLL
                     QGILNQVGKGLKAVHYETGVKRPEVLARLMQAGDEDVSEMLRNLADEAVKTASQSDAI
                     VFIGGISPNLEGEEMEVKVEGFSGGDRTTLDMPAEQVKLLKRLKDTGKPVVLVLTNGS
                     ALSVGWANENIPAIVEMWYPGEEGGNALADVLFGRYNPAGRLPVTFYNSVDELPAFDD
                     YSMEGRTYKYFRGKPLFPFGYGLSYTTFGYSGIKVDKALAHKSDTLSVTVKIANTGKV
                     DGDEVVQLYFRQPAGLEGQPLKSLLAFKRVNIKAGQTADVRLSFPVSHFRHFDTRAGE
                     YRIASGDYEIFAGGSSDDERVKASITVE"
     gene            15186..15890
                     /locus_tag="PKI35_02495"
     CDS             15186..15890
                     /locus_tag="PKI35_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43278.1"
                     /translation="MPSYSLSKSTYIRGLQCLKSLYLNKHRPFLRDRISDEQLAKFAR
                     GHAVGKTAQQLFPGGVEMPAPSEVSAKKTAKLISQGCPVIYEACFIFDGVISAIDILV
                     LKEDGWSAYEVKSSGSLSETYYNDAFLQNWVMIGSGLQLEHFYLVHRNAGKELGSDLP
                     LEELFVFTEIPVHDPARDIRIELSIGEMKFILSRNHSPAIAPGTWCMHPYPCDFRGFC
                     WKGLSEDQKNKLLYGT"
     gene            15906..16523
                     /locus_tag="PKI35_02500"
     CDS             15906..16523
                     /locus_tag="PKI35_02500"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNX43279.1"
                     /translation="MSNFIDLTRTLYHEMPVFPGEKSPAITLDKTIATDGYNGFRLES
                     NMHSGTHMDAPMHVQVTDRTIDSFDAGLFTGKACVIDVRGLKVIRYQPEWEAKFAGHD
                     IVLFRTGHDRTWDTPAYYGEYPDFESLIAGKLVENGVRIAGFDSPSPDRMPYDFHLVF
                     LHDERFLIESLTNLGALPDNSSFRLYAFPLKVRAEASLVRVVAEI"
     gene            complement(16529..17647)
                     /locus_tag="PKI35_02505"
     CDS             complement(16529..17647)
                     /locus_tag="PKI35_02505"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX43280.1"
                     /translation="MKLLPAFLLFASILLSSSSFAQAGKKTSILVRFTEPVSYTKIYL
                     DTLAARGAAEFTSADAGSDGSFELNFVLRDENLYKLRLDQANFMWLILTPGEKVSVIP
                     SGKKLGPDATVNGSHHTALLYSALLKSRTFDLKRDSLNKEYNNIQSSPKHDSLSTVII
                     RNFTENDSLQKATLAEAIKKEPASLAWMFFTDKFDISGDFPFVDNLDRELIKAYPDNT
                     YVRQYHDQVEMERKTGIGKPAPDISLPGVDGNNLSLSSLKGKVVLLDFWASWCGPCRK
                     ENPNVVNIYNRYHDKGFDVFSVSLDKDKASWLKGISADNLSWPNHVSDLKYWKSEGAA
                     AYGVTSIPYSVLIDRNGIIVAKKLRGQDLEKKVAELLR"
     gene            17819..19387
                     /gene="dnaB"
                     /locus_tag="PKI35_02510"
     CDS             17819..19387
                     /gene="dnaB"
                     /locus_tag="PKI35_02510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNX43281.1"
                     /translation="MEEKSNVLPDVAGRKRVKRPMTSQQAFMEHGKLPPQAVDVEEAV
                     LGAMMLEKDALAAVIDTLNPEAFYKEAHQIIFTAILNLFKDNQPVDILTVTNRLKTEG
                     TLEVVGGPFYITQLTSRIASAANIEFHAGILMQKHLQRELIRTSTETIRDAFEDTTDV
                     FSLLDNAERNLFGISENNLRRRTQLMPDLIRDALVQINVAMKHDGSFTGVPSGFTELD
                     RITGGWQNSDLIITAARPGMGKTAFVLSMARNIAVDNKKPVAIFSLEMTSLQLVTRLI
                     SSEAELSSEKLRKGDLEGYQWEQLNSRIRPLIDAPIYIDDTPALSIFELRAKCRRMKA
                     QYDIQMIIIDYIQLMTTGGEQRGGNREQEISSISRSLKSLAKELNVPVIALSQLNRSV
                     ELRPGNNKKPQLSDLRESGAIEQDADIVNFIYRPEYYHITEDEEGNSTEGKAMIIVAK
                     HRNGATGEIWLRFIRNFARFEDLEAAMISNQESFGMSPNSVFEHQNAFRTIPSKLNDM
                     TDDADTVPDGEPNF"
     gene            19481..20737
                     /locus_tag="PKI35_02515"
     CDS             19481..20737
                     /locus_tag="PKI35_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNX43282.1"
                     /translation="MKKIVFLVLLLFGLIPVRAAYLLIPMDQTQKNHLKAYGIAYWVV
                     KNGAEVSWLLNYEGGSFMCKYDKLIENECVIRGVSYQVIADAQSTAILTEIADPEVNM
                     DEVKLFKAPRIAVYSPKNKQPWDDAVTLALQYAEIPYDVIYDEEVLNGVLPMYDWLHM
                     HHEDFTGQYGKFWAAYHNTSWYKEDVRVNEEMAAKLGFQKVSQMKLAVVKKIREFVAG
                     GGYLFSMCSAPDSYDVALAADGTDICESMFDHDPADPAAQSKLNYDNCFAFRNFKLVT
                     NPYEYEISSIDVSESRKVSPQNDYFTLFEFSAKWDPVPSMLCQDHEQLIHGFRGQTTA
                     FRSEYIKPDVIIMGETKSLGEARYIHGDFGKGTWTFYGGHDPEDYEHRVGDPPTDLNL
                     HPNSPGYRLILNNVLFPAAKKKKQKT"
     gene            complement(20841..21932)
                     /locus_tag="PKI35_02520"
     CDS             complement(20841..21932)
                     /locus_tag="PKI35_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNX43283.1"
                     /translation="MNNITNIVKSRAEYFISGIQQVGIGVTNVREAWKWYKKHFGTDI
                     RIFEDEAVAGLMLPYTDGKPRKRLAALAINMQGGGGFEIWQYKDRIPLPPAAEVQLGD
                     YGINAVKIKSHDVARTYQSFVSSGLSVSAPEEGPGKTENFFLTDPYNNMFQIVGGNSW
                     FKNENKLTGAAYGVIVGVSDMDKSVAFYRDILGYDETVYDTSGVFSDLAQMPGGKGNF
                     RRALLKHKDKRQGAFSPVLGDSQIELLQSLDRKPSSIFKGRLWGDLGFIHLCFDIHGM
                     NALREECLLLGHPFTVDSSTSFDMGEAAGHFSYIEDPDGTLIEFVETHRVPILKKLGW
                     YLNLQKRDPHKSLPRWILKSLAFNRVKDF"
     gene            complement(22087..22887)
                     /locus_tag="PKI35_02525"
     CDS             complement(22087..22887)
                     /locus_tag="PKI35_02525"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX43284.1"
                     /translation="MKKTFDGKTIWITGASSGIGEALAYELASCRTHLILSARRMNEL
                     ERVAARCREAGCKCSIFPIDLSDPVNVETTADKVIAETGAVDILFNNGGISQRSTVID
                     TKIEVDRKIMEIDYFSGVILTKKVLPAMVKNGYGHIIAISSITGIFGFPLRSGYAAAK
                     HALHGFYETLWAELHDKGINVTVVRPGRIRTNISLGAVTGTGKVYGIMDHGQDTGISA
                     ETCAHKILKAVERKKILVNIVGRERLMIFFKKFVPCIFYRLVSKVKPT"
     gene            complement(22960..23463)
                     /locus_tag="PKI35_02530"
     CDS             complement(22960..23463)
                     /locus_tag="PKI35_02530"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNX43285.1"
                     /translation="MKPIISIIVAIAQNNAIGKGNQLLWHISDDLKRFKHLTEGHTVV
                     MGKRTYYSLPKRPLANRRNVVITDLPGETIEGCVMAYSIPDALNQMEEGRENFIIGGG
                     SVYSQFLPLADRLYLTVVHKNFDADTFFDAINFGEWKETWREERQTGEFAYSFVNLER
                     IKGKKRI"
     gene            complement(23475..24269)
                     /locus_tag="PKI35_02535"
     CDS             complement(23475..24269)
                     /locus_tag="PKI35_02535"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNX43286.1"
                     /translation="MKQYHDLLQHILSNGVRKDDRTGTGTVSVFGYQMRFDLSEGFPA
                     LTTKKLHLRSIFHELLWFLQGDTNIRYLHENKVSIWDEWANENGDLGPVYGHQWRKWQ
                     SSDGQVIDQISEVIEMIKKNPDSRRLIVSAWNVGDIPKMALPPCHLLFQFYVANGKLS
                     CQLYQRSADVFIGVPFNIASYSLLTMMMAQVTNLEPGEFVHTFGDAHIYLNHLEQVNL
                     QLSREPRPLPVMKLNPEVKNIFRFRYEDFELTGYDPYPSIKAPVAI"
     gene            complement(24290..25540)
                     /locus_tag="PKI35_02540"
     CDS             complement(24290..25540)
                     /locus_tag="PKI35_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HNX43287.1"
                     /translation="MNLKLRLIAMNFLQFFIWGSWLLTIGTYCFQTKKWSGTEFGAIF
                     STMGIASLFMPAIAGIIADRWINAERLYGIFHICGACILFTIPTLNSPDTLFWMMLLN
                     MVFYMPTIALAITVAYTSLKKNDLDIVRDYPPIRVWGTIGFIVALWTISISKLEASPV
                     QFYVSAGASLLLGLYAFTLPKCAPPAKGHKRDLASELGLNAFKLFKNPKMALFFIFSM
                     LLGAALQLTNAYGDTFLHDFAKIPAYKDLIAVKYPAIIMSISQISETLFILAIPFFLR
                     KFGIKKVMLFSMVAWVLRFGLFAYGNPADGLWMIVLSCIIYGMAFDFFNISGSIFVET
                     QIDPSIRASAQGLFMMMTNGFGAFFGSRISGIVIDKYYKTDDLFNWHGIWIAFAAYSL
                     VIAVLFAILFKHKHKPGDMATITH"
     gene            complement(25566..26159)
                     /locus_tag="PKI35_02545"
     CDS             complement(25566..26159)
                     /locus_tag="PKI35_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNX43288.1"
                     /translation="MQKIELKILGLSYSQTQSNSYALILGEKNGHRRLPIVIGHFEAQ
                     SIALEIEKMKPARPLTHDLFKNFADTFNIGIEEVIINKFQEGIFFALLVCNDGVETKQ
                     IDSRTSDAVALAIRFGCPIYTYEPIMAEAGILFEEEEESAEKEQPGGDEAISEFRSMT
                     NEELEEELHKAVDEENYEKASQIRDEINKRKPRQGNR"
     gene            complement(26393..27580)
                     /locus_tag="PKI35_02550"
     CDS             complement(26393..27580)
                     /locus_tag="PKI35_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNX43289.1"
                     /translation="MSENQFYVEVNVVHKNHDLERICGDHFLSRRIYEENRIIAVLSD
                     GMGHGVKAAMLSTLTATMAMNLTEEHKDVHRIADIIMNTLPVCSERKMSYSTFTIIDI
                     EIDGLTRILEYDNPQALVFRGNKQTDPDWETITLVSEKNAGKELRTCSFIPQKEDRIV
                     ICSDGVTQSGMGSAKYPFGYGLDNMSKYVLDLIGNDSTISGAQLAAKVVNMAVMNDNY
                     RPKDDTSCAVIYFREPRRLLICTGPPYEKSKDAELGETVKNFDGKKIICGATTGDIIS
                     RELGVPIVDSFEFDDTDLPPISFMDGIDLVTEGILTLGKVSEILKNYSQKYKPGKGPA
                     DQIVRMLLDSDQIHFIIGTRINIAHQDPTLPVELEIRRTVVRRIVRLLEDKFLKEVTM
                     NFI"
     gene            complement(27625..29472)
                     /locus_tag="PKI35_02555"
     CDS             complement(27625..29472)
                     /locus_tag="PKI35_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNX43290.1"
                     /translation="MLHINADLCKECYACIRICPVKAIELRANETVPYIADNRCIGCG
                     SCLEVCHPNAIVYYDSRPEVRELLKAETPVAAILDPSISAEFYDITDYRKFVAMLKQL
                     GFKFVHEAAFGVDLVAREYYELVSNFLGKYYILANCPPIVSLIEKFHPSLIDNLAPLV
                     NPMVASAKIIHNLHGEETRVVFIGPCLGAKTEVLRHDEDGRVDAVLMFDELRRMFEES
                     GVKESTIEYADFDEPSGNKGSLYALSDGILQAAGLSQDMLSGSMVTVDGRDRILTAIS
                     QFEESIDYIRKNFNVFYCEGCIMGPGMKPNGMKLKRRTDVVSYVNKRLSGLEKGKWIK
                     EQNRYSILDLRRSFEANDQRLPPPPKEKVEEILKIIGKGDGEPDLGCKACGYESCRSF
                     AEAVASGIARTDMCVGYSLRSKQEYIRSLKVTNDKLAKTQQALKESEKQALEEKQATQ
                     DALETMQTMLRKLPTAVLIVDESLKIVASNESFITILGEEAADINEIIPGLVGADLKT
                     LLPFNFYKLFTYVLTSADDVVGRDVHLNDKLLNVTIFSIKKNKIAGGVIRDMYMPEVR
                     REEVINRVTEVIEQNLELVQQIAFLLGEGASKTEKMLNSIIESHQSKEE"
     gene            complement(29520..29777)
                     /locus_tag="PKI35_02560"
     CDS             complement(29520..29777)
                     /locus_tag="PKI35_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNX43291.1"
                     /translation="MGDSKKEIIICLGSSCFARGNKSVVKIISDYLKEHNLQNEVKFH
                     GQRCFGKCANGPLLKLNDDFYEKSDPETIIRLLDDFLENSK"
     gene            29958..30419
                     /locus_tag="PKI35_02565"
     CDS             29958..30419
                     /locus_tag="PKI35_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNX43292.1"
                     /translation="MPVSIEDICSKYKQGSREALLSVLQEIQDNFGYLSEEAIIYVGR
                     HLKLPSSKVYGIATFYDQFRFAPKGRYHLQVCSGTTCHIEGSMALLREIDKLLKIKDG
                     QVSRDGLFSLQVVNCMGACSAAPVIAVNGEFHTLMSSADLAGLFDEIRKNA"
     gene            30457..32388
                     /locus_tag="PKI35_02570"
     CDS             30457..32388
                     /locus_tag="PKI35_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNX43293.1"
                     /translation="MNDQEFLITEVLTAKVPAADRQITGRLAEMRHDRVNRPVLYIGA
                     GTCGLVAGADRTLEAAKLYIREKNIDAEIIEVGCIGLCSAEPLMDVQLPGKSRISFCN
                     VDASVVESILDDIFNGIVPADHVLGQFVSPLHEGWTGVEDIILLPFFRYQHRIVLANC
                     GKSDPQRIADYIARGGYQTFLKCLNNYTSEKVCDIIDESGLRGRGGGGYPTGKKWRTA
                     HNAISDQKYLVCNAAESDPGAFMDRAVIEGDPHLLLEGIAIAAYAVGATRAYIYLMAD
                     YHLALKNLRNAIAQMKEYGLLGYNIYKSGTDLQINVVVSPGAFVCGEETALIGSIQGK
                     RAMPTPKPPYPAIEGLFKKPTVINNVETLANVPYILRHGPQAFNSIGTANSKGTKVFA
                     ITGKSASIGLVEVAMGTTLKDLIFRIAGGIKDDKQFKAVQIGGPSGSCVPEKQLDLQV
                     DYESLWEAGAIMGSGGMVVMDENTCMVDMVKYFMDFLQEESCGKCIPCREGTRRIHEI
                     LENITRRPPSESGHEVLERFKGIMQLEGLAEVIRDTSLCGLGQTAPNPLVSAMKWFRD
                     EFEEHIFDRRCRAGVCKELRVFYIEVEKCTGCVACLRKCPTNAIVGQPRQPHFIIEEK
                     CIGCGICFDVCKFVAITIK"
     gene            32448..34175
                     /locus_tag="PKI35_02575"
     CDS             32448..34175
                     /locus_tag="PKI35_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HNX43294.1"
                     /translation="MMFFDIEVNGKTIKARNGETILSALERNAISVPTLCHIRGLLPS
                     GACRMCVVEVEGKPGLLPACSHPVEEWMKIRTHSPRVIKARKTNIELLLSNHPDDCLY
                     CESSGHCELQKFAEDLYVRERRFYGKKNKYKTDPSSASIFRDPAKCILCGRCIRVCEE
                     VQNISALDFIGRGNEMQVGPAFNKGMNLSSCINCGQCILACPTGALTEKKHPAELQEK
                     LHNPAIKTVIQCSPTLSVTLAEEFGLRPGKDMEGVMYAVLRKIGFDYVFDTSFAADLY
                     LAELAHELLNRIKTGEKFPLFSSCCPAWIKYAEQFRPALLEQISSCKSPQQMMGAIIS
                     NHFTQYRKLQIENIYSVSAMPCIAKKFESQREQMTHKGITDVDMVLTTRELARLIRLN
                     GIDVQQVEPEMADQPYRSRSSSGKMLAVSGGLTEAVIRTLHTLITGKELPLSRIPELR
                     SSKEVKEFFIKIGDYKLGFAVINGIGNAASYLDEIETGRDDIHFVEVMACPGGCVGGG
                     GQPSGRNGAAVKSRAKAIYEIDDKEPVKTPYRNPSINALYESFLHNPGSDESLNLLYT
                     AYQKREVLL"
     gene            34209..36242
                     /locus_tag="PKI35_02580"
     CDS             34209..36242
                     /locus_tag="PKI35_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNX43295.1"
                     /translation="MEDATLNINQPNPGMTKLVFTVKEKCKLCYTCVRECPAKAIRIT
                     GGQAEVITERCIACGNCVKVCSQNAKVFRREIDPVTKLIQSGAKVIAMVAPSFPAEFS
                     EVPDHRLLVGMIRQLGFRHVLEVAFGADLVARKYKEQLNSGIPAISSDCPAIVSFIER
                     YYPELAGNLAEVVSPMVAMARVARSIYGVDSILVFIGPCLAKKAESKEIDNALTFREL
                     REMFDINRINLKMVLPSEFDPPLSGRGAIFPLSHGLLNTMEVTEDILSENVLVAGGKP
                     NFADALREFEQGNLKGHHIHLLCCEGCIMGPGMSPYLNTAAASNRFAKKAAIIRYANA
                     KMATINREEWQENVDEYSKLDLSRTFKPNDMRVVRPSNEEIRGVMYRMGKYGPGDYLN
                     CGACGYDSCEEHAIAIISGLAETEMCLPFSIERLHKYIRELNISNEKLATVQEALRHS
                     EKLAGMGQLSAGIAHELNNPLGVITMYSNILREEVPAEDPIQKDLELIVEQADRCRKI
                     VGGLLNFARKNQVNIAETDVVKLAEHSINSVIVPTDVTLGLENHLRDRTAMLDFDQMT
                     QVLTNLIKNSIEAMPDGGKITLGLTDTEDKIMIRVTDNGTGIPAEHMDKLYTPFFTTK
                     AIGKGTGLGLPIIYGIVKMHKGDIKVESNADPSAGATGTTFTLIFPRQRAVIQ"
     gene            36305..36691
                     /locus_tag="PKI35_02585"
     CDS             36305..36691
                     /locus_tag="PKI35_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX43296.1"
                     /translation="MKKRILIVDDDIDYIFQMKMKVEQMGFETITAESQKEAEKIIEE
                     VKPDLCILDLMMETDDSGFILSYKIRRKYPDVPIIIATAVTAETGMTFDVMAEENKQW
                     INADLFLDKGIRADQLQREIKKLLKM"
     gene            36695..37822
                     /locus_tag="PKI35_02590"
     CDS             36695..37822
                     /locus_tag="PKI35_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNX43297.1"
                     /translation="MATFKILVVDDELGIREGTKRILQNFKVDYPFMDEQIDFQILEA
                     ATGSEGIEIIDTQQPDILLLDNKLPDIQGVEVLEYVKNKHYDIIVVMITSYASLELAV
                     KATRDGAFDFIPKPFTPQEIRASIENITKQLFLKRMTQSLNSTGKQIRFQFLSVLSHE
                     LKAPLNAIDGYLHMIREKQFGNNVDAYTEIIDRSMERIKGMRQLILDLLDLTKIETGK
                     PTQKLLPVDVSKIVQMAIDTNRPYAIQKDVDIYLNNKSAVMMKADAGEIEIIMNNLIS
                     NAVKYNRTGGRVDCFIEKLPEAVRISVSDTGIGMTEEETQKIFEDFVRIRNQQTRDIT
                     GSGLGLSIVKKIVDMYKGAVDVQSTPGKGTTFTITLPVPEN"
     gene            37927..38547
                     /locus_tag="PKI35_02595"
     CDS             37927..38547
                     /locus_tag="PKI35_02595"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNX43298.1"
                     /translation="MKQLPDKTVERLSQYRRTLINILAENKHHIFSHELAALLHITPV
                     QVRRDIMLIGYSGTLRQGYDVKELIEIIGKIIDSREGQRVAVVGIGNLGRSIIGYFSG
                     KRTKLTIVAGFDINPEKVDRVYGGVWCYDFSRLDEIIKKENINIGVITVPASEAAAVA
                     DALVMAGVKGILNFTPRPLNVPSSVYLEEYDIITSLEKIAYFVKKM"
     gene            38641..39582
                     /locus_tag="PKI35_02600"
     CDS             38641..39582
                     /locus_tag="PKI35_02600"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008531 - riboflavin kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FMN
                     adenylyltransferase"
                     /protein_id="HNX43299.1"
                     /translation="MRIFHGFDELTNIPNPVATTGSFDGVHVGHKAIINRVNRLAQEI
                     GGESVLITFHPHPRKVLFPETAGKDLFLINSQREKIDLLKEAGLNNLVVVEFTKEFSE
                     ISSVDFIRNLLVAKLHARKIVIGFNHQFGHNREGNFDFLNELGKYYGFDVEEIPEQDI
                     HNETVSSTLIRKALQEGRIQRANAYLDHHYMIIGKAVKGSTLMSDLEYPTFYTAIEEE
                     CKLVPPDGVYAIRIENAGKSSKAIVNIRNNRYGPGRNFSDLTIEVFPFEQPGIVNGKD
                     VRLFFAKRIRDELSFSNPVDLKRQLEKDRELVGELIF"
     gene            39654..40412
                     /locus_tag="PKI35_02605"
     CDS             39654..40412
                     /locus_tag="PKI35_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX43300.1"
                     /translation="MKVTFLGTGTSTGVPVIACNCGVCTSHDARDRRYRTSALLTIGD
                     TNIVIDCGPDFRNQMLKNRVENIDAVLFTHAHRDHIAGLDDIRAFNYILNKIINIYGT
                     RDTLESIKMQFPYIFSGGFFRVPQITLNTIDDKPFTISGIEFNPICVMHQDLPVTGYR
                     IGDFSYITDANYIEAKELDKMKGSKVIVLNALRNSRHVSHYCLSEALEILDILKPERA
                     YLTHISHFLGKHEEVELKLPPNVKLAYDNLVIEV"
     gene            40545..41981
                     /gene="hydG"
                     /locus_tag="PKI35_02610"
     CDS             40545..41981
                     /gene="hydG"
                     /locus_tag="PKI35_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNX43301.1"
                     /translation="MKFTPEKCRIPDQRMKPFIDADEIWEYLNTVNPDRQQVKDVIAK
                     SLDKKRLSMAETAVLINATDPDLVGEIKEGARTLKERVYGNRIVLFAPLYIGNKCSNN
                     CSYCGFKSSNTAAIRKTLDDQELIREVEALEDNGQKRLILVYGEHRDYSPEFIAHTVN
                     VVYGVKKGHGEIRRVNINAAPLEIEGFRTVKAAGIGTYQIFQETYHPEVYLHYHLGGA
                     KRNYEYRLTSLDRAQEAGIDDVGIGALLGLYDWRFETMGLIRHTNHLEACYNVGPHTI
                     SFPRIQEASAINFDGKYKVSDSDFSKMVAILRLAVPYTGMILTARENPELRREVMHFG
                     VSQIDGGTKLEIGAYSDSINEKQDLNREQFHIGDSRSLNEIIDELIDTGYLPSFCTAC
                     YRLGRTGEHFMEFSVPGFIKRYCSPNAILTLTEYLEDYAPAETRRKGYLAIEKNLLEL
                     EAAAEKIDVPEIRRRIEKIRAGQRDLYF"
     gene            42426..42710
                     /locus_tag="PKI35_02615"
     CDS             42426..42710
                     /locus_tag="PKI35_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43302.1"
                     /translation="MSQIRIIGLFIADRIKEAGLTQHVLAKHSRIIRARLGFHELNED
                     ICSRNGIILLNLTGTTEEQDRLLNDLKEISGIEVREMTFSSDSVNVSNCG"
     gene            42751..43011
                     /locus_tag="PKI35_02620"
     CDS             42751..43011
                     /locus_tag="PKI35_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43303.1"
                     /translation="MKEIRILGIYVNRSKDDTQRIQEILTRYGCSIRTRLGLHDTDEE
                     YYDDRGLILLELYGNDEEAVRLENELLEIDYVEVQKMTFQKK"
     gene            complement(43035..44330)
                     /locus_tag="PKI35_02625"
     CDS             complement(43035..44330)
                     /locus_tag="PKI35_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNX43304.1"
                     /translation="MYSLRGKLSKLLIISFLFFTFTPVLSVQSQTLPVETGTATESVC
                     PGINYAIPVTVKNMIGVDSLQLTLTFDPLVLNYITYRAVNTQLTGGKFNIKSSAGEVI
                     ISWVRKSQAASIINDTLAELIFFTSSGTTPLAFDELNCFYYMPDGSLLTDEYFTGSVT
                     VFGKVSMVLTEIDPTCANSCDANFMVNASGGKPPYTYLWNGSPGRFDTIETNLCSGSN
                     EMLITDANGCEFDSMFTVNGMPGANVKLIIEPDSIIYLQNPTLSFRFDEISPTHVTEV
                     PLWDFGDGDTARSFNPTHTFATADQNKDGYFNLSLLVKNENGCDSLIQMKLPIKEATI
                     DIPNVITPNSDGMNDAFMIVNKDKQHDDWIISNEYKRLELYVFDRWGRRVYATDDYHG
                     DWTAKGLPDGVYYYVLKLVGYYKTSKKKGSITILGSGVK"
     gene            complement(44517..44966)
                     /locus_tag="PKI35_02630"
     CDS             complement(44517..44966)
                     /locus_tag="PKI35_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43305.1"
                     /translation="MDTALKKRLIEICRQKLTDSGINLQQAMDDLQQQSNEYGAPKDR
                     YDPFRTQLMRRRDMLAQQLAKELQELKMLDKIDLSRSYREVGFGAIVTTNDFSYFIAV
                     GLGKVETTDGTFYAISTGVPLYEALQGKKEGDTVNFRGKNITLKQIG"
     gene            complement(45166..46509)
                     /locus_tag="PKI35_02635"
     CDS             complement(45166..46509)
                     /locus_tag="PKI35_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX43306.1"
                     /translation="MVRLSLAALFGLLFRGIKTHSAHHRLPYCLMVLLLSAPAFGQGT
                     AKRCLFLGNSYTYANNLPRMISDIAASTGDTLFFDSNTPGGYTLHEHSTNAASLAKIM
                     SGNLDYVVLQEQSQLPSFPLSQVMTEVFPYASYLDSIINAYNACSETVFYMTWGRKNG
                     DDENCNWWPPVCTYEGMDSLLNLRYRMMADSNEAILSPAGAVWHEIRQYYPSIELYQS
                     DGSHPSVAGTYATACCFYTTLFRKNPELITYDASLPAADAATIRNIVKSVVYDSLYKW
                     HIGEYDPSAGFSCTINGSNQVAFSNTSLNAHEYLWDFGDGDTSAEINPVHNYAVPGIY
                     TVSLIASHCSLKDTLRKAVNTTETGIPSPEIQSWNIFPNPATVKFTVSGLKPGSYPYM
                     IYDFSGRKIQSGEVSGANVQVDISGLPDGIYLLKLSGNEKITEQRKLIKCSHNHP"
     gene            complement(46525..47211)
                     /locus_tag="PKI35_02640"
     CDS             complement(46525..47211)
                     /locus_tag="PKI35_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNX43307.1"
                     /translation="MRERIHDFLTKPIVIALVILISAVLTHFTGGLGYFFGMIAALMV
                     LWSGRFKWIEFGISRPRWIKSILVAIALAISIFIIVDILIQPLIELKFGAIDLSSFDG
                     IRGNYINFLVFILFMWVVAGIGEEFLYRGFFMKQFARILGDTNKGWLFSAIAMSILFG
                     MAHLYQGMSGMISTGLVSLCLSLIFYKNRNNLILNMFVHGFYDMIGISLIFINKERIF
                     TDWIQAFLIQ"
     gene            complement(47345..47788)
                     /locus_tag="PKI35_02645"
     CDS             complement(47345..47788)
                     /locus_tag="PKI35_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNX43308.1"
                     /translation="MKPEHDKAIKLFRSGYNCAQSVLAAYSEDMKFDAGLALCISCGF
                     GGGMGRLQQTCGAVTGSFMAIGIYNCKKYSDNNERKDKTYAMIRQFNEKFMALHGSTD
                     CKSLLNCDLMSEEGRLFAKQNNLYEMRCEKYISDAVGIIQELTGE"
     gene            complement(47908..48822)
                     /locus_tag="PKI35_02650"
     CDS             complement(47908..48822)
                     /locus_tag="PKI35_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX43309.1"
                     /translation="MKKEILLMFCLCISIFTVNAGEKYITTSDGVKLYVDVRGSGPAC
                     LYLHGGPGSGSYWLEKYYGNSLEQHFQMIYLDQRGVGRSTSPKDGNFSMARMIMDFEE
                     VRESLGIKHWLTLGHSFGGILQMGYVTGKPGSIDGLIFINCCLSMNDCFKKSWLPKAI
                     ELAGQDTPPICLDTNVNIFERMGAISQVLNEKGTMWKLFFASEENGNKMNDTYLNFPE
                     WNSDFSNKSMEIADYWTNFCLLTPQVTQPVLFFYGKTDWAVGPSHYKEIAFPNMLLWG
                     SDVGHMPFFENTEDLDKAIDSFVSKYHF"
     gene            complement(49123..49893)
                     /locus_tag="PKI35_02655"
     CDS             complement(49123..49893)
                     /locus_tag="PKI35_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43310.1"
                     /translation="MKKANHNIAAYTLVMAFIAVAFATSCRPSKESGITGSETFQKIT
                     GNINGSGKALELTFTKGPSFNHPTFAIWLEDTSGRFIQTLFITRSFGSGTFYYGDKSG
                     GQWKPGQVKMPASLPYWSHKAGAAKGIANYIPDKKNPVPDAYTGATPKGNFALETRSD
                     KGTPRFVKVFMEINQTWDWNEYWTNNKYPDDTEYKTSCQPAVVYAAVIDMEKPGETAV
                     LKPIGRSHHSGADGNLYTDLSTLSTALHIAEKITAEVK"
     gene            complement(49908..52301)
                     /locus_tag="PKI35_02660"
     CDS             complement(49908..52301)
                     /locus_tag="PKI35_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX43311.1"
                     /translation="MRINHILIQFLAFIPVIVFGQTGVIKGRVYNASSNEPLPFTNII
                     ILGTNIGSVSDLDGNFLFTAVAPGFFKLAATSVGFENVITEELQVTNARTTFIDIAMK
                     EKPFQLAQVEVISSPFKKVEESPVSLKTIGIAEIEKSAGANRDISKVIQSLPGVSSGV
                     SYRNDLIVRGGGPSENRFLLDGIEIPNLNHFGTQGASGGSVGIINSDFLREVDLYTGA
                     FPASRGNALSSILDMRLIDGNPEKMIYRATLGASEMAASASGPLSKKTTLLFSARRSY
                     LQFLFSALGLPFLPTFNDYTIKVKTRFDLKNELTILSIGSLDKSTLNTGIENPTEEQQ
                     YILNYLPSYDQWTYAIGAVYRHYRKNSYDTWVLSRNMLNNRSKKFENNDKNAGLVLKY
                     ESEEIENKLRYENTTRLNGYKILTGAGLEYIKYLNNTFRKVFIPTEKDTLTSLDYDSF
                     LDFFRYNAFGQVSKSYLENRLSLSAGIRLDGNSYSAGMSNPLNQFSPRISASYGITDK
                     VFLNANAGRFFQLPSYTTLGYRDETGKLVNRDNGVTYIRADHLVAGLEYRRNDNSRVS
                     LEGFLKFYENYPFSVKDSVSLASKGADFGVYGDEAVKSTSGGRAFGAEFYYRGRFMNH
                     VNVILSYTFVRSEFDDKKGDLIPSAWDNRHILNIMASADLGRNWNIGAKWRFVGGAPY
                     TPYDYNRSSLIEAWDASGQGYPDYNLYNTERLANFHQLDLRIDKEYYFRKWSLNVYFD
                     VQNAYNFKSKNPDVLVLLRDANGNPVIDPNDPARYSLKYLPSESGTILPTLGVIIEL"
     gene            complement(52803..53447)
                     /locus_tag="PKI35_02665"
     CDS             complement(52803..53447)
                     /locus_tag="PKI35_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43312.1"
                     /translation="MPRKALLSFLLLTLLCGCHKEDEKNESVFRGLVIDYDTGNPKPG
                     VTVILNRFDGLVIGFPDRYIGLVQDSALTDENGKYCFKVRENDKFLYKITTRQSGYLV
                     KNPAQATLAKIIDYYPVNSDTIRTGIAGYVKLDVVNIPNDYDSVFINFINAVAEPYRL
                     YPQLHDTTLINGFLCKDNPTVNVHWKITDKPESNTGSATVSLAPQDTVTVRIEF"
     gene            complement(53509..54204)
                     /locus_tag="PKI35_02670"
     CDS             complement(53509..54204)
                     /locus_tag="PKI35_02670"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNX43313.1"
                     /translation="MKEKKVKSPQQSIVLNLFSKDNLLICGILSTILYVAATILGAIR
                     WDEYDSASQTVSELFAIGAPSSSVIVPAFLIYSLLVFAFGAGIWISAGNQKTLRIVAI
                     LVISKEVLGVIGLLFAPMHMRGMGTSLTDLMHIVITITGVLLCMFPAIIFGAFSFGKG
                     FRIFSFITIVIFLVFGTLAGMEGSKIAENQPTPYAGIWERINIFTYFIWVVVLSIILL
                     KRNRTQAGQIDPQ"
BASE COUNT        14102 a        12750 c        13460 g        14444 t
ORIGIN      
        1 ggtataggta tgggttcctg cagcctgggt gaaatcgccg gttccggtgt aaggagcggc
       61 gccgccggtg gcagaaatgg taacggtgga gttgccgccg ttgcaggcga tggcagtatg
      121 cgattcgccg gcaaccagga tttcaggttg tgaaatggta acagtcacat cgtcggagca
      181 accgttggca tcggtgacgg tataggtatg ggttcctgca gcctgggtga aatctccggt
      241 tccggtgtaa ggagcggcgc cgccggtggc agaaatggta acggtggagt tgccgccgtt
      301 gcaggcgatg gcagtgtgcg attcgccggc aaccaggatt tcaggttgtg aaatggtaac
      361 agtcacatcg tcggagcaac cgttggcatc ggtgacggta taggtatggg ttcctgcagc
      421 ctgggtgaaa tcgccggttc cggtgtaagg agcggcgccg ccggtggcag aaatggtaac
      481 ggtggagttg ccgccgttgc aggcaatggc ggtgtgcgat tcgccggctg tcagcactga
      541 aggctcattg ataatgacat attcatggtc tgactcacag cctccgctga tattctttac
      601 tttgataaag tatgttccgg cgacaaggcc attaaaaatg ttactgttct gataggttgc
      661 tccgtcatct ttcgaatact ggaagtcgct gatagcgcct gaacccggag ttacagcaat
      721 agacccgttg ttgcctgcat agcatgtgac atctgtaacg gatgtagtaa attccggaac
      781 agggttaacc gtgatgacaa cctggtcggt tgaggaacag ttccccgaag cattggttac
      841 tgtaagcgta taggtagtag taaccgaggg agtggctata ggattggcaa tgttggtgta
      901 attcaatccg gttgacgggc tccaactgta gagaacaccc ccggttccgt tcagctgtgt
      961 gctttcgccg ttgcagatgg aaacatcctg cccggcttgg gcgactatag cagtcgggct
     1021 tccgattgta gcagttgcag taacgttggg atagcaactg cccgggtccc tgacaagtat
     1081 ggtgtacgtc ccggctgata gtcccgtaaa agtattaatg ttttgccatg ttgaaccatt
     1141 attgatggaa tactgatagc tacctgaccc gcctgaaacg ttaacggtaa tcgttccgtt
     1201 gctttgtcca aagcattgaa gattcagagc tgataccgag gtgatatttg ccagttcata
     1261 aaggtggatg gtctgggtta cataaatctg gtttgactcg cacggacaac tgacaggagc
     1321 cttgacaata aaagtctcgt cattatctga gatgccatcc gatactgcag agtaaggaat
     1381 atcaatatag gcaacattgg cagggaaagt aatctgcgtc gggaatggct gattgtctga
     1441 cgtgagaata tctgtgccat tggtggcagt tcctgaaata ttcagattta ccgtcagagg
     1501 agcggatata tcggaaccgg aacgttcaat tctcaaataa tcattgcatc cttcgaaaat
     1561 attgttctgg ccttcattaa agctgccata attgttcaga gcgtagttgg tcagggaaat
     1621 attttgagaa ttaaaacttt tagctcctaa aaatacaccg gcatcccatt cctggtcaga
     1681 agcatctgca atactcatcc tgatcctgta agtagaacaa ggctgaacag cgtaggtagc
     1741 tttcagaaca acggttccgc catcatattg cattgtaagt gaacctgagg cgttggaaaa
     1801 gtaataggtt tcattcgttg ccgggaatgt gccataatta ttggttactg ccggatgaat
     1861 agtattgatc gaaacgggag ttgtggttcc tggaattgta gccagattta cggcattgtt
     1921 tgtgtaaggt ccggagatgc cggggccgct caggaaaaat ccaaaggcgt cgttgtattg
     1981 cgaaccaaca tattcaagat attcttccga agtaaaaaca aaggtgaatt cgatggagtc
     2041 gcctgcgggt acgaaatcaa attccagcac agcagcatca tacatagtcc ttccggtgat
     2101 agtggccaga tcgggatcgg aggcattgct gaccttttta tctgatttcc cggtattgtt
     2161 gttggggccc atggccgacg atgcattgcc ggtgcaaagg agcaatcctt cctcgagggg
     2221 gaatgatgat gttcctttac tgaaatatgc cagttggcgg tttccgggag tgcccgacca
     2281 ggtgtgattt acccatgtgt tattattgtt tctgttataa taaccaaaac gtacattgct
     2341 ggcagtcagg cagccggtaa ccagaacatt ctgaaccaat tgctctgccg aatatgcatt
     2401 ataggttgca ttctcagcaa ctgagataga accggcaggg gcgtcagaat tattcccggc
     2461 cctataggct gatgtaaagg gagaatcagg cggcggtata cggtttgcca ctgagctatt
     2521 ctctttactt acttgtccgt tgacgttcag cgccaaaatc attattacta gcaatggcag
     2581 cctcttcagg ttgacgttat ttaaaatcat aaatgacgat ccttttgtat taaaggccga
     2641 cttccgatta attaatagag aatttttcat gggtattatg gttttaccaa tggctgattc
     2701 agttcgacta gaggcggtgt tttcttcctg ataattgttt aaccgggaca ggaatgaacc
     2761 cagggaagat ggaataacca atcagattgt tttcaaaatg aaacaaccgt gaaactggtt
     2821 ttctgttgtt acggatggga acagtgaaag gcttaataaa aaaatatccc tgataaaaca
     2881 tactgatacc agataatgag cagtatgtca gttgcttatt aattcgtttt aacagagtat
     2941 cgtgagtaag cgttttcaat gtttgtgcgt aagacaggtg aatattgagt agtgttttga
     3001 ttatttattt ttttggaatc atctgatcat ccaatttctt gccaaaaatt gtaacatgct
     3061 atacttcatg ttattatgtt tggttgcctc tgctaatccg gcttttcaga tttcattttg
     3121 aaaaaaaaat ttaattcagt aaaataattg tgtgggatga aaaatgctgt tctgaatttt
     3181 tcatttcatg cggagtaaaa tgacgttact tctggtccaa tcatttcaga ttgagtggat
     3241 attcaatgag atcaatgatg ttcagatagg attattatca aaggattccg tccgggatta
     3301 aactatttcc tgcaataaat gaagggaaac gaattctttt ggcaatcatt tgactggttt
     3361 cttttcggtt catctctgcg aaatagttcc aaaatgggac aatgttcgct cctgcgttta
     3421 attttggaaa gctgaccacg aaataattac caggaaactt tccatggccg atggaagctg
     3481 gcccaattcc ggtactgata aattcatcat gccgaccaac gtgttgcccc gacatagtat
     3541 caaaaggaag cggccgatca attcaaaata ttgattaaca aatattaaca atgacagaag
     3601 gcgatctgta cctatttttg ccgttcagac aatatgaaag ccggaattac cactttactg
     3661 atcatttttg ttttaatctc ccgcgcccgg gggcaggaac aacctgaaca taatgttcgc
     3721 gtggatggtt ttgttaccac catcaggggt aatgcgttgc aatacgttaa tatcattaac
     3781 cttaataagc gcacaggaac gataaccgat gcaaaaggat cgttccggat tgcagcctct
     3841 ccggatgata cgctggtgtt cagttccgtg ggatttcaga acaagaaaat gcgggtacaa
     3901 ccctatcccg gcaatccggt ttgccctgtt cacatatatc tgcaggagga caccattacg
     3961 ctttccgggg tcactattca tgactggccg aggaacctga cggcgttgcg ccaggctgtc
     4021 cttgctcaga aaattgagga accggccata gctgatttaa acctgaatgt tgtagtaccg
     4081 gatgcccctc agccaggttc tccgataacg ctgccgggta tgggtaatcc gggactcacc
     4141 tatacgattc cgggaccgat aactgccttg tacaatgctt tcagccgcag gggaaagtcg
     4201 atgcagcgat atgccgaact gacccggcag gacaggatcc atgaaatgat agcaaggaaa
     4261 tataatcctg aaatagtaaa agacattacc ggattcaaat ctgatgaaga aatcattgcc
     4321 ttcatggatt actgtaacct ctcggtcagt ttcctgctga acgcaactga atacgagatc
     4381 ttatgctcgg tgaaagactg tctggcgaac tatctcgccg aaaaacaaca gtaaggttgc
     4441 actgatcagg ggaagcaggg tatatcgccc tccatggatg catccagtag ttccttcaca
     4501 gtcgaaatta acagaccaac agaggtatct gcggcgccgt aatagatctt tacttcgctg
     4561 ctttctatgg caaaaccttc ccggtcgtat gcttccacta tcataccgct ggggaaagtt
     4621 acattcggca cctgcccggc gagttcccag atttcccttg gttccagtat attgcagcga
     4681 caacggccca atacttttga aggatctttt aagtcaagca gcatgactcc gccctggtaa
     4741 atgttggcgc ttccgaaatg cccggcaacg ccgtggtaaa catgaagcca gcctttgcgg
     4801 gtctttacag gcggcggacc ggaaccgata aattcgtccc agtaatgaaa ccggccgtca
     4861 atcagaggcg ctaccggcgt ccagtcgata aggttgtccg atccgctgag ccagatggtg
     4921 ctgcctgagg tcggtccccc cgtgtgcctg gctttgttcg gacggtccat acggagatat
     4981 ttcccgttga ctttttcagg gaaaagcaca ccgttgcgga tatcttcatt ggatgtgagg
     5041 ccaaggaaat cgaaatccct gaagtcatcg gtgactccca gtcccaattg gcagccgtct
     5101 tccatatcca tggcgaacat gatgtaaaaa cggccttcaa ggtttgtgat ccgtgcatca
     5161 tacgtgtgaa agatcctgcc tttcacattt tcgatccctt tgaaatgcac gatcctgttt
     5221 tccacactga agttcacgcc gtcagggctt tgcgccatta ccagaaatgt ttcccgggaa
     5281 cggccctgaa cgcgcaacat caaacggtaa atatttccgt ccttgatggc gccgggattg
     5341 aaggcgctgg tggcatcgac caattccggg ggaatagggg gaatgtcatc gcgggtaatg
     5401 atcggatttt taggatgccg tttcatatca gtcagtattg gtttaattaa tgcataatga
     5461 cttttacccg gcaggtttcg gcagatgata cagggatcag gtttccttca atggcattgc
     5521 cgtcaacaaa tacccggctt actccttttt cggaaccgtt cgggttctca atttcaaaga
     5581 tataccttgt ttgccggtaa acacgctcgg ctttgaacga tttccagtgc gatggaatgg
     5641 cgggatcgat aatcagaccg tcataggttg accggatgcc gagaatataa tcgtagctca
     5701 cccggtacat ccatgccgca gaaccggtct gccatgaatg gcttgcccgg cccggctgcg
     5761 aatgttcgtt gctggtgata tactgcgaat aaacataagg ctcggctaca aaggtttcgc
     5821 tgtcgatacg gttcggcagc atttttttaa aatattcaaa tgcctggttg ctgcgtccca
     5881 gcatagtttc ggcctggatg acccatgttg tagggtggca gaatacagcg ccgttttcct
     5941 ttttacccca gacgcagcgg gtgacaaggc caatttttgg atctatttca cgccaggccg
     6001 gcgtgcattt tttggggcct tccggcgaat cgagatattt ctttacgctg tccatggcgg
     6061 taaccaggcg atcccggttt ggaaaacctg caattaccgg ccatatctgg gtgttgagga
     6121 atatcttccc gtacttgttg tcctttgaac cgacacgacg gtctttttca ccgaaggcgc
     6181 ggatatacca ctcgccgtcc cagcagtttt cttcaacggc tttcaccagg ttttcacgga
     6241 cggcggctac ttctgattgc gctgttgtca tcctgaggcg gccaagcaat tcaatgaaaa
     6301 ggttgagcat ggcggcatag aacatgcctc cccaaacact ttcgcctcct tcgtcgccgc
     6361 cgatataatc aagagtatcg ttccagtcgc cacggccaaa gatgggtaac ccgtgcttgc
     6421 ccaggttgtt tttggcatag ttcacaacgc tgaacatgtg gtccaggata gtatcgggat
     6481 cggcatcaat aaatggctgt ttatcatgaa gaagatcaaa gtcgccggtt tccttgaggt
     6541 attccgttac cgccagcatg aaccaaagcg gatcgtcgca atggcaggtg aattcgcctt
     6601 gcccgtctcc gtggaaatgg tggtacacct tgccgtcctt gcgcatctgt cgcgaaagca
     6661 actgaatacg ttcttttgct ttccccgggt ttgagatggt cacggcaatt gtatcctgcg
     6721 atgtatcgcg gaatccgaag ccccggccga tcccccagta ataaaaactg atcacacggc
     6781 caacatcaaa agcaactttg gcctgataag gcaaccagta tcgcatgaat atattcacgt
     6841 catcatcagg agtgtcaacg gtggtatacg aaaagtattt atcccacgtc cggttaagct
     6901 ttgcgaatgc agcatcaact tcagcaaccg acttatactt ctgaaccctt tgttctttca
     6961 tctccccgaa cctgattttt tcaatcactc ccagcgagaa aaccaggtcg gtggtttcac
     7021 cgggattcag ttcaatatcc accttcagcg catttactac atttccgaaa tcggtgtcct
     7081 gacatgaaag gttttccttt tctatgcttt cagggttgtt ttcgttccgg tacagcccga
     7141 gaaaacgttc ccgcagggtt tcaaaacctg taacgggcct gttcacggtg aaaaatgtcc
     7201 actggtccca ctccttgttt tcctgttgct gtgtgccgct cgtttcggta acccaatagg
     7261 ttttggtgga gaaaagggcg ttcaggtcct tctcgaaatg ggaacggttg aaatgctggt
     7321 cgtcgcactg gttgatcaga tccaccatgg catggcccag ggctaattct acatagccgt
     7381 aaacagatag tttccgggat ttcctgcccg tatttttcag gcttgctttc caaatttcct
     7441 ggtcatcatc ttctggaacg aagaacagga cggatgaatc tatatcattg gccgaagctt
     7501 cggctctggt atagccgaaa ccatgggcta ctttatatgc ttcggcgtgc ttcccgacag
     7561 gttgccacgt aaggctccag atttcgccgg tttcgttatc cctgatgtaa atatatttcc
     7621 cgggtcggtc ggggggcact tcgttgatcc ggtaccttgt gatacgtccg tgcaaagggc
     7681 ttttataata actgattcct ccgccgttgt tcgaaatggt ggaaaaataa cgcccgttgt
     7741 aaatataatt gatccagggg ctgggcgtgc gcgggttggt gatcagatat tctttgccgt
     7801 ttttgatgaa gtgtccgtaa ttcataactc attttttaat gtttgttcac aaaaccgtcg
     7861 tcatgatcat ccctgatatt tgtccgtaag gcatggatca tctctttcag gtttttaata
     7921 ccgcctatgg tgaaccagat ggtcacaatg atggatgccg tcagaaagac gtaggtatat
     7981 actttccaga aattcatcca ctgattgttg gtgaatggct tcacgaagaa gtagaagagc
     8041 gtcccgataa tgaatacgac tacccaggct gccgtccagc cggttgttac ataatagatc
     8101 gctttgtcgc ctttggtgaa ttctttggtc ggggctaaca ctttccagcc tttgataact
     8161 tccgtgtcgg acgatgagag ttcggtctga acggcatatt cgccacggtg caacaattta
     8221 tcaaggttgt atgcttttcc gggggtgagt aacgaaacga ttacatatac cagcgaagca
     8281 gataccatgg cgataagcca gccccattgt ccgttgatgg ggaaatcggt gatgatctgg
     8341 ttgagcagga tgttgaccga tgaggcggtt gccccggtga tcatggccgc ccaggctgcc
     8401 ggggtagtgc ctttcttcca ataaaggccg ccgatgatga cggcgccgga gcctcccaca
     8461 aaaatggccc ctgaaatatt caggaacagg aatacttttt ccgtttgctg gaacaggatg
     8521 ctcaggatga aaatcgtgat cccgacgccg aggatcgaga gtttcaggta tttaagatgc
     8581 tctttgggct cgaagggttt ctgcctgatc ggcactacta catcctggat aaagatgctt
     8641 ccccaggagt gcatatacgt attatgacag gtgatggctg ctgccagcat gatggccgcg
     8701 aaagcgcctt tcaatcctac aggcagcata tagttgagga tcatgggaac acggagctgg
     8761 ctctggtcgg cgggggcgaa accggccagc atgttgttga tggaatcggc gattactttg
     8821 tagttctcgt ggtggaaaat ggtgtaagcg acaatcggga cgatcatcag gaataatccc
     8881 cattgcggta ccagcctcca gtttgtaagc acatccgcca tcttggcttc gtgtgcgttc
     8941 ttggcggcaa tgctgaacga ggaattgccc tgccatgaga gtttggtata tataattcct
     9001 atcatcccga taaagaagaa ccatggatta aaatcctgaa cttcgcttgc atcgaaagga
     9061 ttgatcagcg acttaccggt gggagccgct tttacggctt caccgatgtc aacccagcct
     9121 acctgaacca gcagcagtat cacgatgatc acaaaaacga tattcacaaa aacgccctgg
     9181 atgaagtcgg tgaacatgac ggcgatgtgc ccccccgtaa agatgaagta caggggaacc
     9241 agcaccatca ccgcagttgt taaaatgaat gtaccggagt atggtccgat gaggccgatt
     9301 tcgggtatcc cgcagaaata aatgaaaaat ttggcagtta cggatgggaa gatgatcata
     9361 ttcacgatgc ctgatagaaa ggccagcatc ccggcgaata tcctgaaagt cctgctgtat
     9421 ctcatttcga agaattgagc catggtaagg gcccgtgttt gtcggaaacg gtatacaacc
     9481 cagcctgtga cgctgatggc aacaagaatg acggaggttg gcatctccca ccaccgggaa
     9541 ttaaaacctg cattgtaatt ctgctccagg gcgcctacca cggtaatggc ccctaaggcg
     9601 gcggtgccgt gaaacatgga tatgatgtag cgcccgcctg tacgccctgt tgccaggaaa
     9661 tcggtcacgc tttttaaaag gcttttgctc aggaacacca tgccaagcat gattgaaaac
     9721 aagacgatga tgatactcca gtcgatccag gtcaggttca tagcagatgg tctgttaagg
     9781 gttaatgaaa aggtgtttat gacttagctg gtcttatttc ggttttaatt tatccagcac
     9841 aggctcggcg aggcctcttg acaggttgat ttccggatcc agcatttcga ggacgaacat
     9901 ttcgttgact ccttttttca ggaaagttgc aggacaatac agcgtttgct ggggccctat
     9961 attccagtac cttccgagat tgttgccgtt tatgaatacc acacccttgc cccaaaggct
    10021 catatcaatg aatgtatccc ctgttttgtc aagtgtgaaa gtgccgcgac ggtacatggg
    10081 tccggccggt tttttcttct cagttctggc agcggcaaaa tccggtaatt tggtcatcgg
    10141 caaggggaaa atctcccagt ttttcaactc ctgtttcccg agaatcacat tgccggttat
    10201 accttttcga ttatcggtca ttctcctgcc gtaattgatg cggcccatat tttcgaccag
    10261 gatttcaagt ttgttgttgg ctgtcctggt ttgaatttct atcgaatcct gttttaaccg
    10321 gcggtcaagg gtgccgaccg gaatcccgtt tatcatgact accgcaaaat cccttaatcc
    10381 gttgattttc aacattgatt ttccgggctt tgcgatggtt gtccggtata atatatagcc
    10441 atatccctgg ttcacctctt ccattgtcag aactgaggcc gatctgatgg gtttgggcaa
    10501 caggctggtc aggctgtgaa cctcgctgag cctgaaatag gggatactta tgaccggcaa
    10561 acgttcggga acttcaggga ttagcgtccc ttcaggcaga aatggaaaga tgagtttttt
    10621 aatggcatag tacttttcgg tcggccatcc ggcttcatta accggcgcat cataatcgta
    10681 gctcgtgata tcgggctgaa tgggaaattc attattgtag ttggcgccat tcataaagcc
    10741 gaagttcgtt ccgccatgca gcatgtaaaa gttgaaagaa aatccggaat tgaggagtgt
    10801 ttttatgtcc tttaccacat cgtcggaatt tacgtcagac ttcggctcgc cccaatggtc
    10861 aagccagccg ggataatatt caggaataaa gtaaggcccg gagccattgt tatatttatt
    10921 aaccgtatcc ataagggcat caggatcggt ttccccgttg atagccggca ggataccggg
    10981 caggtatccg tccttgcatt gaacaggccc gtcggccgtg aacagcggta ccgtaaaacc
    11041 ggctttgacg aacatatcgc ggatcagcga catgtattcc ttgtcatttc cgtatgagcc
    11101 atattcgttt tcaacctgcg ccatgataat cggtccgccg ttggtcacct ggagatcacg
    11161 tacctggttg ccgatttcca tgacgtattt ttccgctgca gcaagaaaac gcgggtcttt
    11221 acttcgtaca accatatctt tttccctgag cagccaccag ggatatccgc caaattccca
    11281 ttcggcacag gcataaggcc cgggacgcag gattacccac aggccttcgg cttgcgccat
    11341 cctgatgaat gtcgccaggt cgcggttgcc ggttttaaag tcgaatttcc cgggcatggg
    11401 ttcatgatcg ttccagaaac agtaaacaga gatggtgtta acgcccatgg catgagcggt
    11461 ttgcagccgg ttttgccagt attcgcgggg tatacgctgg taatgcattt cgccggaaat
    11521 gatctgcaaa ggctttccgt ccagtttgaa atccatacct tcagccgtga aagtgtgtga
    11581 cggattttgt gcaggaactg caaaagggat cagggatata caggtgatga caaacctcag
    11641 gaaaagtttc atgacgggtt ttggagttat gttttattga atcgaatcgg caacggagaa
    11701 cttccagcgt gtttcggaag agtacatatt ccccggtcgc aggatcacat tcgggaaacc
    11761 gggaatattc atggaattgg gatagtgttg ggtctcggta caaaaagctg catggggcat
    11821 atatggaact ccgcctttac cggtgatgct gccgtcgaac cagttggcag tatacaactg
    11881 catgccgggt tccgtggtga aaacttccat caccctgccg cttcccggat gaaagaccct
    11941 ggcagccggt gcaagcttgc ccgatagatt cctgagacaa taattgtcgt catagccttt
    12001 gtaaagttcc ccgatccgtt ccccgatggt atgcggggta gtaaaatcaa aagcagttcc
    12061 ggcgacaggc ttcaggaaac cggttggaat aatgttcccg tcaacctctg taatctgatc
    12121 ggcaagaata acgagctgat ggtccagaat atcgcccgaa ccttcaccgc gcaggttcca
    12181 gtaagaatgg ttggtcatgt tgaccggtgt gggtttgtcg gtcatggcat aataggatac
    12241 cgataactca ttgttgttat tcagcgaata aacaaccttt acctgcaggt tgccggggaa
    12301 tccttcctcg ccgtcgggac tgaaatactc caggattaca gaaacatcat catcggtact
    12361 cactgctttt gatttccagg cttttttgtt gaagccttca aatccaccat gcaactggaa
    12421 agttcccttg ttcggcgata ccttatattc tgtaccgtca atcttaaatg ccgagccggc
    12481 aatgcggtta caggtacgtc ctacaataca gccaaagtat ggttctgtat tcataatcag
    12541 tttttcaaca gcgtcataac acagcagcac atcgtccgtc ttgccgttct tgtccgggat
    12601 ccaggcttcg gtaagcgagc cgccgtaatt ggtcagcctg attacaatcc catggtcgtt
    12661 ctcgagcgtg tatctgaata cctcttcacc cgtgggaagg ttgcatatat gcagggaatt
    12721 gattttcatt gctgtctgat tatggttttt acagtaaatt tgaaggtcgg ctcttttaca
    12781 tagtaaagat aaagaacctg aatggcattt accaacctgt tgctaaaaaa tacaaattta
    12841 gtaacttgct tttgtattga tgctaataat taaaaaagat caataagttt tataagctta
    12901 gtaataaact taatgaaagt tacgatgagg agcattacca gaaaattatc ggggttagtg
    12961 ctgatgatgg tattcaacat gctgtcaatc agtctttttt cgcaaaacca ggaagccggg
    13021 acagaaaatt ttcttttttt gaatccaaaa atttcaccgg aagaccgtgt caatgacctg
    13081 gtcggtaagc taacgctcga agagaaaata ttacagatgc agcacgatgc tccggctatt
    13141 ccgcggctgg gaattccgga gtataactgg tggaacgaat gtctgcacgg ggtagcccga
    13201 aatggcattg caacggtatt tcctcaggcc atcggcatgg cggccacctg ggatccgggg
    13261 cttattcaca gggaggccga tattatcgct acggaagcca gggcgaaata tgctgaagcc
    13321 atcggtaaag gggaacatgg tatttatcag ggacttacct tctggtcgcc taatatcaac
    13381 atattccgcg atccgcgatg gggaaggggg caggaaacat acggcgaaga cccccgtctg
    13441 acaaccgaaa tcgggaaagc tttcgtcaca gggcttcagg gcgatgatcc caggtattac
    13501 aaagtgattt caacggcaaa acattttgcc gttcacagcg gaccagaatc cgaacgtcat
    13561 cgttttgatg catggccttc catgcgcgat ctctacgaaa cctaccttcc ggctttcgaa
    13621 gccctggtga aagaagcaaa agtgtattcg gtgatgagtt gttacaatcg tgtttatgct
    13681 acccccactt cgggaaatac tttcttgctg aaagatatcc ttcgcgatac ctggggtttc
    13741 aaaggttata tagtgtctga ctgctgggct gtttccgatt tctataattt ccacaatttc
    13801 gtgccttctg ccgataaagc ttcggcgctg gctgtgaggg ctggttgcga ccttgcctgc
    13861 ggcaacgaat atgttcacct gaacgaagcc atcaaaagcg gctacctcac cgaagccgat
    13921 ctggatgttg ccctcagaag ggtatttatg gcgcggatga agctggggat gttcgatcct
    13981 ccggccctgg tgccctatgc ttctattccg ccatcggcaa ataacaccga agatcaccga
    14041 caacttgcac gccaggtcgc ctgcgaaagc gtcgtccttc ttaagaatga acataacctg
    14101 cttcccttct caaaaaaaat aaggactgtt gccgttatcg gcccttatgc caacgatacc
    14161 tcggttttgc tgggcaatta caatggtacg ccgtcggcgc cggtcaccct gttgcaggga
    14221 atactcaacc aggtgggcaa aggtttgaaa gctgtccatt atgaaacagg cgtgaaaagg
    14281 cccgaagtac tggccaggct tatgcaggca ggtgatgaag atgtgtctga aatgctgagg
    14341 aatctggctg acgaagccgt gaaaaccgct tctcagtcag atgccattgt tttcatcggc
    14401 ggtatttctc caaacctgga aggggaagaa atggaagtga aagtggaagg cttttccggc
    14461 ggcgaccgca ctaccctgga tatgccggct gaacaggtga aattgctcaa aaggctcaaa
    14521 gataccggga agcctgtagt attggtattg accaacggaa gtgcattgtc agtcggctgg
    14581 gcaaatgaaa atatccccgc cattgtcgag atgtggtatc cgggcgaaga aggcgggaat
    14641 gccctggctg atgtcctttt cggacgctat aaccctgccg ggaggctgcc ggttaccttt
    14701 tataattcgg tggatgaact tcccgctttc gatgattatt cgatggaagg acgtacctat
    14761 aaatatttca gggggaaacc gctgttcccg ttcggctatg ggctgagtta cactacgttt
    14821 ggttattcag gcataaaggt cgataaagcc ctggcccaca aaagcgatac cctctccgtc
    14881 accgtgaaaa ttgccaacac gggaaaggtt gacggcgacg aggtggtaca gctttatttc
    14941 aggcaaccgg cagggctcga agggcaaccc ttgaaatcgt tgctggcatt caaacgggtt
    15001 aatataaaag caggtcaaac ggctgatgtg aggctttcgt tcccggtttc acatttcagg
    15061 catttcgata cccgggcggg cgaatacagg attgcttcag gggattatga aatttttgcc
    15121 ggaggctctt cggatgacga aagggtgaaa gcttcaatca cggttgagta aagttttcag
    15181 gtgacatgcc ttcttattcg ctttcgaaat ccacatatat cagggggctg caatgcctga
    15241 aatcgctgta cctgaataaa cacaggccgt ttctgcgcga caggatcagc gatgagcaac
    15301 tggcgaaatt cgcgcgtggc catgccgtag ggaaaaccgc tcagcagctt ttcccgggag
    15361 gcgttgaaat gccggctcct tcagaagtat cggcaaaaaa aactgcaaaa ttgatttcac
    15421 agggttgtcc ggtgatttat gaagcatgct tcatttttga tggagtgatc tcggccattg
    15481 atatcctggt actgaaagaa gatggctgga gcgcatatga agtgaaaagc agcggttcgc
    15541 tgagcgaaac ctattacaac gatgctttcc ttcagaattg ggtgatgatc gggagcggac
    15601 ttcaactgga acatttttac cttgttcacc ggaatgccgg aaaagaactg ggaagcgatc
    15661 tgccgctcga agaactgttt gtctttactg agattccggt tcatgacccg gctcgtgata
    15721 tccggataga attatcgatt ggtgaaatga aatttattct ttcccggaac cattcgcctg
    15781 ccattgctcc gggtacctgg tgcatgcatc catacccctg cgatttcagg ggattttgct
    15841 ggaaaggact ttctgaagat caaaaaaata aacttcttta cgggacttaa atcaaacagc
    15901 tcaatatgtc aaactttata gatctcaccc gcacattgta ccatgagatg ccggttttcc
    15961 cgggtgaaaa aagtccggcg atcactctcg acaaaaccat tgcaaccgat gggtataacg
    16021 gattccggct tgaatcgaac atgcattcgg gaacccacat ggatgctccc atgcatgtac
    16081 aggtcaccga ccgtaccatc gacagcttcg atgccggcct ctttaccggg aaagcctgcg
    16141 tgatcgatgt aaggggctta aaggttataa ggtatcagcc tgaatgggaa gcaaaatttg
    16201 ccgggcacga tattgttctt ttccgcactg gccatgaccg gacctgggat acgccggctt
    16261 actacggtga ataccccgat tttgaaagtt tgatagccgg gaagctggta gaaaacggtg
    16321 tgcggattgc cggattcgat tcgccttctc ccgaccgcat gccctatgat ttccacctgg
    16381 ttttccttca tgacgaaagg ttcctgatcg aaagcctcac caatctgggc gctttgcccg
    16441 acaatagcag tttcaggttg tacgccttcc cgttgaaagt ccgggccgaa gcttcgctgg
    16501 taagggttgt agccgaaatt tgaggaagtt aacgaagtaa ttcggcgact tttttctcca
    16561 gatcctggcc gcgtaatttt ttggctacaa taatcccgtt tcggtcgata aggacactat
    16621 aaggaataga agtaacgcca taggcggcgg cgccttccga tttccagtat ttgaggtcgc
    16681 ttacgtgatt gggccaggaa aggttgtcgg cagagatccc tttcagccat gaagccttgt
    16741 ccttatcaag cgatacgctg aacacgtcaa agcctttgtc gtgataacga ttataaatat
    16801 ttaccacatt cggattttcc ttgcggcagg gcccgcacca ggaagcccag aaatcgagta
    16861 aaaccacttt gcctttcagc gacgacaggc tcaggttatt gccgtcaacg ccgggaagcg
    16921 aaatatcggg ggccggtttc ccgatgccgg ttttccgttc catctcaacc tggtcgtggt
    16981 actgtcttac ataggtatta tccgggtatg ctttgataag ttccctgtca agattgtcaa
    17041 caaagggaaa atcgcctgaa atatcgaact tgtcggtgaa aaacatccat gccagcgacg
    17101 caggctcctt ttttatggct tctgccaggg tggccttttg cagcgaatcg ttctccgtaa
    17161 aatttcggat gattactgtt gacaatgaat cgtgtttcgg cgaggactgt atgttgttat
    17221 attctttgtt gaggctgtcg cgcttcaggt caaacgtcct ggattttaac aaggcggaat
    17281 agagcaacgc ggtatgatgc gaaccattta ccgttgcatc ggggcccagc tttttcccgc
    17341 tgggaataac gctcactttt tccccgggcg taaggatcag ccacatgaaa ttggcctggt
    17401 caaggcgcag cttatacaga ttttcgtcac ggagcacaaa attcagttcg aaactgccgt
    17461 cgctgccggc atcagcgctg gtaaattcgg cggcgccccg tgcagccagc gtatcaaggt
    17521 atatttttgt atatgatacg ggttcggtaa atctgaccag gatagaggtc ttttttcctg
    17581 cctgggcaaa agaagaggaa ctgagcaaaa tggatgcaaa aagcagaaaa gccggtaaca
    17641 atttcatgag cagcggtatt gatataggat tatacagcaa agataggttt ttgttcattt
    17701 gaaggtgcct gtaaatgaat tcaaatgagc ggccgaaagc atcttttatg ccattgttca
    17761 taacttattg ggcggttctg ctgtgggaaa tgcaatcaaa attcatactt ttggccatat
    17821 ggaagaaaag tccaatgtat taccggatgt tgccggacgc aagagggtga agaggcctat
    17881 gacctcccag caggcattta tggaacacgg caaacttccg ccgcaggccg tggatgttga
    17941 agaagcagtg ctgggggcca tgatgctcga aaaggatgca ttggcagccg tgattgatac
    18001 gctgaaccct gaagccttct ataaagaggc gcatcagatt atcttcacgg ccatactgaa
    18061 cttgtttaag gacaatcagc cggtagatat cctcacggtt acaaacagat tgaaaaccga
    18121 aggaacactt gaggtcgtcg gaggcccgtt ttatatcaca cagcttacca gccgcattgc
    18181 atcggctgcc aatattgagt ttcatgccgg gattctgatg cagaagcacc tccagcggga
    18241 gctgatccgt acttccaccg aaacgatccg cgatgccttt gaagatacaa ccgatgtttt
    18301 ctccctgctc gacaatgccg aacgcaacct tttcgggatc agtgaaaaca accttcgccg
    18361 ccgtacccag ctaatgcccg acctgatccg cgatgccctt gtgcagatca acgtggccat
    18421 gaagcacgac gggagtttta ccggcgttcc ttccggcttt accgaactcg accgcataac
    18481 cggaggctgg caaaactccg atctcatcat cacggcggcc cgtcctggta tggggaaaac
    18541 ggcgttcgta ctctcaatgg cacggaatat cgccgtcgat aacaaaaaac cggtcgcgat
    18601 attctcgctc gaaatgacca gcctgcaact ggttaccagg ctcatttcat ccgaagcgga
    18661 actgtcatcg gaaaaactgc gcaaaggcga cctggaaggc taccagtggg aacaattgaa
    18721 ttcaaggata cgccccctga tcgatgcgcc gatttatatc gacgatactc cggcgctttc
    18781 aattttcgag ttgcgggcaa agtgccggcg tatgaaagcg caatacgata ttcagatgat
    18841 tatcatcgac tacatccagt tgatgacgac cggcggggaa cagcgcggcg gcaacaggga
    18901 acaggagata tcgtctatct ccaggtcgct caaaagcctg gccaaagagc tgaatgttcc
    18961 ggtgattgcc ctttcgcagt tgaaccgttc cgtagaactg aggccgggga acaataaaaa
    19021 gccccaactg tcggaccttc gcgaatccgg agctattgaa caggatgccg atattgtcaa
    19081 ttttatctat cgtccggaat attatcacat taccgaagac gaagaaggca attccaccga
    19141 gggcaaggcc atgatcatcg ttgccaaaca ccgtaacggc gccaccggcg aaatatggct
    19201 caggtttatc agaaattttg cccgtttcga agacctcgaa gccgccatga tcagcaacca
    19261 ggaatcgttt ggaatgtcgc ccaattccgt tttcgaacat cagaatgcat tccggactat
    19321 cccgtcaaaa ctgaacgata tgacggatga tgcggatact gttccggatg gtgaaccaaa
    19381 tttctgattt cttgttgccc aatcaataat ttacaggtac ctgcgttagt accttcatcc
    19441 gggatgatac tgacagatcc cgtatttatt gtagccggtt atgaaaaaga ttgttttcct
    19501 ggtactattg ctattcggct tgattccggt tcgggctgca tacctgctca tcccgatgga
    19561 ccagacccag aaaaaccatt tgaaagccta tggcattgcc tactgggtgg tgaaaaacgg
    19621 ggcggaagta agctggctgc ttaattacga aggcggcagt tttatgtgta agtatgataa
    19681 actgatcgaa aacgaatgtg tgatccgcgg ggttagttac caggtgatcg ccgatgccca
    19741 atcaaccgcc attcttaccg agattgccga ccccgaggtt aatatggatg aagtaaagct
    19801 tttcaaggct cccaggatag ctgtctattc gccgaaaaac aagcagccct gggatgatgc
    19861 ggtaacactt gccttgcaat atgccgagat accttatgat gtaatttatg acgaggaagt
    19921 gctgaacggc gttttgccca tgtacgactg gctgcacatg caccacgagg attttaccgg
    19981 tcagtatggc aaattctggg cggcgtatca caatacgtcc tggtacaagg aagatgtcag
    20041 agttaacgag gaaatggctg caaagctggg ctttcagaag gtttcacaaa tgaaactggc
    20101 ggtggtcaaa aaaatcaggg aatttgtagc cggtggggga tatctgttct ccatgtgttc
    20161 ggctcccgac tcatacgatg tggcgctggc tgccgacggc accgatattt gtgagtcaat
    20221 gtttgatcac gatcctgccg atcctgccgc gcaatccaaa cttaattatg acaactgctt
    20281 cgcattccgc aattttaaac tggtgacaaa tccttatgaa tacgaaattt catctatcga
    20341 tgtttcggaa tcacgaaaag taagtcccca gaacgattat tttaccctgt tcgaattttc
    20401 agccaagtgg gatcccgttc cttccatgct ctgtcaggac catgagcagc taatccatgg
    20461 tttcagggga cagaccacag cattccgcag cgaatatatc aaacccgacg taattatcat
    20521 gggggaaacc aaatcactgg gcgaggcgcg gtatattcac ggcgatttcg gcaaagggac
    20581 ctggaccttt tatggaggcc atgacccgga agattatgag catcgcgtcg gcgacccgcc
    20641 taccgattta aatcttcatc ccaattcacc gggctaccgc ctgatcctga acaatgtgct
    20701 attcccggct gccaaaaaga agaaacagaa aacctaaaaa cgtttgaaga agcgattgaa
    20761 caatggaccg gctcccggat gtcattgcag aggctttaaa tactctgatt tttaaatttt
    20821 taaatcctca aattttcaaa ttaaaaatct ttcaccctgt tgaaggccag cgatttgagt
    20881 atccatcgcg gtaacgattt gtgcgggtcg cgtttctgaa ggttcaggta ccagcccagt
    20941 tttttcaata tcggcacacg gtgtgtctca acaaattcta tcaaagttcc gtcagggtct
    21001 tcgatatagg agaaatgccc ggcggcttcg cccatgtcaa aactggtgga gctgtcaacg
    21061 gtaaaaggat ggcccagtaa gaggcactct tcccgtaagg cattcatgcc atgaatatcg
    21121 aagcaaaggt gaatgaatcc caggtcgccc caaagccggc ccttgaaaat ggacgacggt
    21181 ttacggtcga gcgactgaag cagttctatc tggctgtcgc ctaaaaccgg gctgaaagcg
    21241 ccctgccttt tatctttatg tttcagcaag gcccttctga aattcccttt gccgcccggc
    21301 atctgggcca gatcgctgaa cactcccgaa gtgtcatata ccgtttcatc atacccgaga
    21361 atgtcgcggt aaaaagcaac agatttgtcc atgtccgaaa cgccgacaat cacgccatag
    21421 gcggcgccag tcaatttgtt ttcgttcttg aaccatgagt tgccgccgac aatctggaac
    21481 atgttgttgt aaggatcggt gagaaagaaa ttttcggttt ttccggggcc ctcttccgga
    21541 gcggatacgc tcagtccgct ggaaacaaag gactgatatg tcctggcaac gtcgtggctt
    21601 tttattttaa cggcattgat cccatagtcg cccaactgca cttcggcggc aggaggcaaa
    21661 ggaatgcggt ccttatactg ccagatttcg aatccgccgc caccctgcat attgatcgcc
    21721 agcgccgcca gcctttttcg cggttttccg tcggtatatg gaagcatgag gccggctaca
    21781 gcttcatcct caaaaatgcg gatatcggtg ccgaagtgtt ttttatacca tttccatgct
    21841 tcccgcacgt tggttacgcc gatccctacc tgttgtatgc cgctgatgaa atattcggct
    21901 ctcgacttca ctatgttggt tatgttattc atgatctcaa ttttactgta ttggatgaaa
    21961 cctgcaacaa accgggccat ccgggtttta gaggcatcat tatgagtgac tacggccggc
    22021 ttatccttgc ctcactttca gaattccggt atccggatta gttttcaacc ttctcacggc
    22081 ctttcctcag gttggtttaa ctttcgatac aagcctgtaa aaaatgcagg ggacaaattt
    22141 cttgaaaaag atcataagcc tttcgcggcc aacaatatta accaggatct tttttcgttc
    22201 tactgctttc aggattttat gagcgcatgt ttctgcactg atgcccgtat cctgtccgtg
    22261 atccatgata ccgtacacct ttcccgtacc ggttaccgcc ccaaggctga tattggtgcg
    22321 tatccgtccc ggacggacaa ccgtaacatt gattcccttg tcatgaagct cggcccatag
    22381 ggtttcataa aaaccatgaa gggcatgttt tgccgcagca taacccgacc tgagcgggaa
    22441 cccgaatatc ccggttatac tgctgatggc tatgatatgg ccataaccgt tttttaccat
    22501 ggcgggcagg acttttttag tcaggatcac accgctgaaa taatcaatct ccataatttt
    22561 ccggtcgact tctatctttg tgtcgatcac ggtcgaccgc tggcttatgc ccccgttatt
    22621 gaaaaggata tcaacagcgc ctgtttctgc aattactttg tcggcagtgg tttccacatt
    22681 caccgggtcg gaaaggtcga tcggaaatat gctgcatttg cagccggctt ccctgcacct
    22741 ggcagcaacc cgctccagtt cgttcatgcg ccgtgccgaa agaatgagat gggtgcgaca
    22801 ggatgccagc tcataggcca gggcttcacc aatgcctgat gaagcgccgg ttatccagat
    22861 cgttttcccg tcaaaggttt tcttcatagt cgtttacgag attgtttcag ttaaataaaa
    22921 aggggagcat cgtttttgcc tgatcatcat cgaaacaggc taaattcttt ttttgccttt
    22981 gatccgttca aggtttacga aagaataggc gaactccccg gtttggcgct cttcgcgcca
    23041 ggtctccttc cactcgccga agttgatcgc gtcgaaaaag gtatcggcgt caaaattctt
    23101 atgaaccact gtaaggtaaa gcctgtcggc caatggcagg aactggctgt aaaccgaacc
    23161 gccgccgatg atgaaatttt ccctgccttc ttccatctgg ttcagcgcat cgggaataga
    23221 gtaagccatc acacagccct caatggtttc cccgggcaga tcggtgataa cgacgttgcg
    23281 acggttggca agaggccgtt tgggcaggga ataataggtg cgtttcccca ttaccaccgt
    23341 gtgcccttcg gtaagatgtt taaaccgttt caggtcatcc gaaatgtgcc acagaagctg
    23401 gttgcccttc ccgatggcgt tgttttgcgc gatagctacg ataatggaaa tgataggctt
    23461 catggcagtt ttttttatat ggcaacaggc gccttaattg agggataagg atcatagccg
    23521 gtcagctcaa aatcttcgta ccggaacctg aaaatgttct ttacctccgg gttgagcttc
    23581 atcacaggaa gcggcctcgg ttccctcgaa agttgcaggt taacctgctc caggtggtta
    23641 aggtatatat gggcatcgcc gaaagtatgg acaaattcgc ccggctcaag gtttgttacc
    23701 tgcgccatca tcatggtgag cagggaatag gaagctatgt taaacggtac cccgatgaaa
    23761 acatcggcgc tccgctggta caactggcag gagagtttgc cattggcgac ataaaactgg
    23821 aacaggagat ggcagggagg cagggccatt ttaggaatat cgccgacatt ccaggcgctg
    23881 acgatcagcc tccgggaatc gggattcttt ttgatcattt caattacctc gctgatctgg
    23941 tcaatcacct gtccgtcgga ggattgccac ttgcgccact gatgtccgta aacaggtccc
    24001 aggtcgccgt tttcattggc ccattcgtcc cagatgctga ccttgttttc gtgaaggtag
    24061 cggatgttgg tatcgccttg caggaaccac aacaactcat gaaagatcga acgcaggtgc
    24121 agtttcttgg tggtgagtgc cggaaaccct tccgacaggt cgaaacgcat ctggtagcca
    24181 aatacgctga cagtgcctgt gccggtgcga tcatctttcc ttaccccgtt acttaaaatg
    24241 tgctgcagca ggtcgtgata ttgtttcatt taaccgttta ctgttattat tagtgcgtga
    24301 tggtagccat atcgcccggt ttgtgcttat gcttaaaaag gattgcaaat aatactgcga
    24361 tcaccagtga ataggcggca aaagcaatcc agattccgtg ccagttgaat aaatcgtccg
    24421 tcttatagta tttatcaata acgatgccgc tgatacggct tccgaagaag gctccgaacc
    24481 cgtttgtcat catcatgaac aatccctggg cgctggccct gatcgacgga tcaatctgtg
    24541 tttcaacaaa tatggaccct gaaatgttga agaaatcgaa ggccatgcca tagatgatgc
    24601 aggaaaggac gatcatccaa agaccgtcgg cagggttgcc gtatgcaaac agcccgaaac
    24661 gcagcaccca ggctaccatg ctgaacagca tcaccttctt gatgccgaat ttgcgcagga
    24721 aaaaggggat tgccaggata aagagcgttt ccgaaatctg ggaaatagac ataatgatgg
    24781 ccggatattt gacggcaatc aggtctttgt aagccggtat tttggcaaaa tcgtggagga
    24841 atgtatcccc gtaagcgttt gtgagctgaa gggcagcccc cagtaacatg gagaagatga
    24901 agaacaaagc catcttcggg ttcttgaaca acttaaatgc atttaacccc aattcgcttg
    24961 ccaggtctct tttgtgccct tttgccggcg gagcgcattt gggaagggtg aaagcgtaaa
    25021 gtccgagtag caatgaagct cctgccgaaa cataaaactg gaccggagag gcttctagct
    25081 tactgatact gatggtccat aatgccacga taaacccgat ggtgccccaa actcgtatgg
    25141 gaggataatc cctgacgata tcaaggtcgt tctttttaag ggacgtatag gcaaccgtga
    25201 tagccagggc gatggtcggc atataaaaga ccatgttgag cagcatcatc caaaacagtg
    25261 tatccgggct gtttaaggtc gggatggtaa acaggatgca ggcgccgcat atgtggaata
    25321 ttccgtaaag cctttcggca ttgatccagc ggtcggcaat gatcccggca atggcaggca
    25381 taaacaggga ggcaatgccc atagtggaaa atattgcacc aaattcagtc cctgaccact
    25441 tttttgtttg aaaacaataa gttccgatag tgagaagcca tgatccccag atgaaaaact
    25501 ggagaaaatt catggcaata aggcggagtt ttaaattcat tgagattcta tttgtaaagg
    25561 cagcgttatc tgttgccctg acggggtttg cgtttgttaa tttcgtctct tatctgtgaa
    25621 gctttctcat agttttcttc gtcaacggct ttatgcagtt cctcttccag ttcctcgttc
    25681 gtcatacttc tgaattctga tatggcttcg tctccgccgg gttgttcctt ctcggcagat
    25741 tcttcttcct cttcaaataa aataccggct tccgccatta tgggttcgta ggtataaatg
    25801 gggcagccga accggattgc cagggcgacg gcatcagagg tgcgtgaatc tatctgtttg
    25861 gtttcaacgc catcgttgca aaccagtaac gcgaaaaata taccttcctg gaatttattg
    25921 ataatgacct cctcaatgcc gatattgaat gtgtcggcaa aattcttgaa aagatcatgg
    25981 gtgagcggcc tggccggctt cattttttca atctcgaggg caatggattg tgcctcgaaa
    26041 tgacctatta cgatggggag gcgccgatgg ccgttctttt ccccgaggat aagggcatat
    26101 gaattggact gggtctggct ataggataac cccaatattt ttagttcaat cttctgcatc
    26161 tggtcttagc ctggcttttc tctcattcaa acaaaaagta aaaataggaa gaattcgttt
    26221 cagatggctt tacagtcaaa agatttcacc tgtcatctga atccccgtaa ccgaaacctg
    26281 cttctttccc ggatgatata tggctggtac tctggattgg aggcccttgc cgagatgacc
    26341 ggaaatacca tttggatgtt gttcccgtgt ttgcaatgct tgcgcgcacg gtttaaataa
    26401 aattcatggt aacctctttc aggaatttat cttcgagcaa ccggacaata cggcggacaa
    26461 cggtgcgcct gatctcgagt tctaccggta gtgtcgggtc ctggtgggcg atattgatcc
    26521 ttgttccgat aataaaatga atctggtcgc tgtcgagcaa catgcgtaca atctgatcgg
    26581 cggggccttt acctggcttg tatttctggg aatagttttt caggatttcg gagactttcc
    26641 caagcgtgag gatgccttcc gtcaccaggt cgatgccatc cataaaggaa atagggggca
    26701 gatcggtatc gtcgaattca aaactatcga caatgggcac tccaagttcc cgtgagatga
    26761 tatcaccggt tgttgccccg catataatct ttttcccgtc gaaattcttt acggtctcgc
    26821 ccagttcggc atctttactt ttctcgtatg gaggaccggt acaaatgagc agcctgcgcg
    26881 gttcccggaa gtagatcaca gcgcaactgg tgtcatcctt ggggcggtaa ttgtcgttca
    26941 tcacggccat attcaccact tttgccgcca gttgggcgcc tgagatagtg ctgtcgttcc
    27001 cgatgaggtc cagcacatat ttcgacatgt tatccaatcc atagccgaac gggtatttgg
    27061 cgcttcccat gcccgattgc gtcactccgt cggaacagat aacgatgcga tcctcctttt
    27121 gcgggataaa gctgcatgtg cggagttcct tgccggcgtt tttttccgaa accagcgtta
    27181 ttgtttccca atccgggtcg gtttgcttgt tgccgcggaa aaccagggcc tgaggattgt
    27241 catattccaa tatgcgcgtt aatccgtcga tttcaatatc aataatggta aaggtgctgt
    27301 aactcatttt ccgctcgctg catacaggca gggtattcat gatgatatcg gcgatgcggt
    27361 gaacgtcctt atgctcctcg gtgaggttca tggccatggt ggcggtaagc gttgacagca
    27421 tggctgcttt tacgccatgc cccatgccgt cgctcagaac ggcgataatc ctgttttctt
    27481 catagatgcg ccttgaaagg aaatggtcgc cgcagatacg ctccagatcg tggtttttat
    27541 gtaccacatt cacttctaca taaaactggt tttcactcat tcggtaattt gttgcatgta
    27601 aatgtgatca atgctaacgt ggcgttattc ttctttcgac tggtgcgatt cgatgatgga
    27661 gttcagcatt ttttcggttt ttgatgctcc ttcgcccaac aggaatgcta tctgctggac
    27721 cagttcgagg ttttgttcta tcacttcggt tacgcggttt atcacttctt ccctgcgtac
    27781 ttcaggcatg tacatatcgc ggatcacgcc tcccgcaatc ttgttttttt tgatggaaaa
    27841 gatggtgaca ttgaggagct tatcattgag gtggacatcg cggcccacaa catcgtcagc
    27901 gctggtcagc acataggtga agagtttgta aaagttgaaa gggagcagcg ttttcaggtc
    27961 ggcgcctacg aggccgggaa ttatctcgtt gatatcggcg gcttcttcgc ccagaatggt
    28021 gatgaaactc tcgttggaag ccacaatttt cagcgattca tccacgatca ggacagccgt
    28081 aggaagtttc ctgagcatgg tctgcatggt ttcaagcgca tcctgtgtcg cctgcttttc
    28141 ttccagcgcc tgtttctccg actctttgag cgcctgctgg gtcttggcaa gtttgtcgtt
    28201 ggtaactttt aacgaccgga tatattcctg cttgctccgc agcgaatagc ctacgcacat
    28261 atcggtccgt gcgataccgc tggcgacggc ttcggcaaac gacctgcagc tttcgtagcc
    28321 gcatgctttg caacccaggt cgggttcgcc gtcacccttg ccgatgatct tcagaatttc
    28381 ttctaccttt tctttaggcg ggggaggaag gcgctggtcg ttggcctcaa aactgcggcg
    28441 gagatccaga atgctgtatc ggttctgttc tttgatccat ttcccttttt ccaacccgga
    28501 taaacgcttg ttgacatagc tcaccacatc ggtcctgcgt ttcagcttca tgccgttggg
    28561 tttcatgcct gggcccatga tgcatccttc gcaataaaaa acattaaaat tctttcggat
    28621 gtaatcaatg ctttcttcaa actgactaat ggccgttagt atccggtcgc ggccgtcaac
    28681 agtcaccatc gatccggaga gcatgtcctg gctcagccct gcagcctgca gaataccgtc
    28741 gctcaaagca taaagagatc ccttattacc ggaaggctca tcaaaatcgg catattcaat
    28801 ggtgctttcc ttaacccccg attcctcaaa cattcttctc aattcatcga acatcagaac
    28861 cgcatcaacc ctcccgtctt catcatggcg caacacttct gttttagcgc caagacacgg
    28921 cccgataaaa actacgcgcg tttcttcacc atgaaggtta tggataattt tggcgctggc
    28981 aaccatcggg tttaccagag gcgcaagatt atcaatgagc gaaggatgga atttttcgat
    29041 cagtgataca atcggagggc aattggcaag gatataatat ttgcccagaa aattgctgac
    29101 cagttcatag tattccctgg ctaccagatc aacgccaaag gctgcttcat gaacgaattt
    29161 aaagccaagc tgctttagca tggcaacaaa tttccggtag tcggtaatgt cgtaaaattc
    29221 agccgatatg ctgggatcga gaatggctgc aaccggcgtt tctgctttca gcaactcccg
    29281 gacttcaggc cttgaatcat agtagacaat tgcattgggg tggcatactt cgagacaact
    29341 gccgcaaccg atgcagcggt tatcggctat ataaggcacc gtttcgtttg cccgcaattc
    29401 aatcgctttt acaggacaaa tgcggataca cgcgtaacac tccttacaca gatccgcatt
    29461 tatatgtaac aaagggcctt gcatcatgtt tttgtaatta gtgacttttt agaaaccggt
    29521 catttgctat tttcaagaaa atcgtccagc agccggataa tagtttcagg atccgatttt
    29581 tcataaaagt cgtcgttgag ttttaacagc ggtccgttgg cgcattttcc gaagcatctt
    29641 tgcccgtgaa atttcacttc attctgaagg ttatgttctt tcaggtaatc gctgattatc
    29701 ttgacaaccg acttgttccc ccttgcaaaa caggaactgc cgaggcaaat gatgatctct
    29761 ttttttgaat ctcccatggt tgcttgaaag ctatctttaa tcagcaggtg aatttacatt
    29821 taattgtaaa acaaaagtaa taaaaatgaa tttgagggtt cgttgttatt tgaataacat
    29881 tgttattttt ttaacaaatt ctaattgaga ttctggctga atcattattt ttgtacctgt
    29941 tacaataaaa atggattatg ccggtaagca tcgaagatat ctgttcgaag tataaacaag
    30001 gcagccgcga agccttactt tctgtattac aggagattca ggataatttc ggctatctat
    30061 ccgaggaagc tatcatctat gtcggcaggc atctgaagct gccatccagt aaagtgtatg
    30121 gcatagctac tttctatgac caattcagat ttgctcccaa aggacgttat cacctgcagg
    30181 tctgcagcgg gacaacctgt catatcgaag gctcaatggc cctgttgcgt gaaattgata
    30241 aattgttgaa aatcaaggat gggcaggtga gccgcgacgg attgttcagc cttcaggttg
    30301 taaattgcat gggggcatgc agcgcagctc cggttattgc cgtcaatggt gaatttcata
    30361 ccctgatgtc atcggccgat cttgccggtt tgttcgatga aatacgtaaa aacgcctgat
    30421 tcccggatac cgggtattaa caacaagtag caacgcatga acgatcagga atttttaatc
    30481 acagaagtcc tcactgccaa agtccccgct gctgaccgtc agattaccgg aagacttgcc
    30541 gaaatgcgtc atgaccgggt gaaccgcccg gtcctgtaca tcggagcggg aacctgcggg
    30601 ctggtagccg gggcggatcg tacccttgaa gctgcaaagc tgtatatccg tgagaaaaat
    30661 atcgatgccg agattatcga agtgggatgc ataggacttt gttctgctga accgttgatg
    30721 gatgttcagc tgcccggtaa atcaaggatt tcattttgta atgtagatgc gtctgtcgtc
    30781 gagagcatcc ttgatgatat attcaatggt attgtccctg ccgatcacgt gctggggcag
    30841 tttgtcagcc ctttgcacga aggctggaca ggggtggagg atataatcct gctgccattt
    30901 ttcagatatc agcaccggat cgtattagcc aattgcggga aatccgatcc tcagcgaatt
    30961 gcggattata tcgcacgggg ggggtaccag acgttcctga aatgccttaa caattatact
    31021 tccgaaaaag tctgtgatat tattgacgag agcggtttac gcggtcgcgg agggggtggg
    31081 tacccaacgg gaaaaaagtg gaggactgcg cataatgcaa tcagcgacca gaaatacctg
    31141 gtatgtaatg cggctgagag cgatcccggc gcttttatgg accgggctgt cattgaaggc
    31201 gatccgcacc tgttgcttga gggaattgcc atcgctgcat acgctgtcgg ggcaaccagg
    31261 gcgtatatat accttatggc cgattaccat ctggccttga aaaacctgcg taatgccatc
    31321 gcgcagatga aggaatacgg cctgttgggg tacaatatct ataagagcgg caccgatctg
    31381 cagatcaatg ttgtagtaag cccgggtgca tttgtttgcg gcgaggaaac tgccctcatc
    31441 ggcagcatcc agggtaaaag ggccatgccg acacccaagc ccccgtaccc tgctattgaa
    31501 gggcttttta agaagccaac agtgattaac aatgtggaaa cactggccaa tgtgccctat
    31561 attctcaggc atggacccca ggccttcaac agcatcggca ctgctaacag caaaggaacg
    31621 aaggtatttg ccataaccgg gaagtcggca tccatcggac tggttgaagt tgctatggga
    31681 acaacgctta aagatctgat tttcagaatt gccggcggga taaaggatga taagcagttt
    31741 aaggctgttc agataggagg gccatcagga agctgcgtcc ccgaaaagca acttgacctt
    31801 caggttgatt atgaatcgct ctgggaagcc ggagctatca tgggatcggg cggaatggtc
    31861 gtgatggatg aaaatacctg catggttgat atggttaaat atttcatgga ctttctgcag
    31921 gaagaaagtt gcggtaaatg catcccctgc cgcgaaggga cgcgccgtat ccatgaaata
    31981 ctggaaaata tcacgcgcag acctcccagc gaatcgggac atgaggtgct tgaaagattt
    32041 aaagggatca tgcaactcga aggcctcgcg gaggtaatcc gtgatacttc gctgtgcggg
    32101 ttggggcaaa cggcgcctaa cccgctggta agcgccatga aatggttccg ggatgaattt
    32161 gaagaacata ttttcgatcg ccgttgcagg gctggggttt gcaaggaatt gagggtgttc
    32221 tacatcgagg tggagaaatg taccggctgt gtggcctgcc ttcgcaaatg cccgaccaat
    32281 gccattgttg gtcagccccg ccagccgcat tttatcattg aagaaaaatg tattggctgc
    32341 ggaatatgtt tcgacgtttg caaatttgtg gcaatcacga taaaatgaac gcttgtttca
    32401 gcttgattaa taactgtcca ttactctcac cataacgaaa tacatccatg atgttttttg
    32461 atatcgaagt caacggaaaa accattaaag cccgcaacgg agagaccata ctctcggcgc
    32521 tggagcggaa tgccatcagt gtgcccacgc tgtgtcatat ccgcgggttg ctgccttcgg
    32581 gcgcctgccg tatgtgcgtg gtggaagtgg aaggcaaacc gggactgttg ccggcatgct
    32641 cccatcctgt ggaagaatgg atgaaaatcc gtacccattc gcccagggtt atcaaagcca
    32701 gaaaaactaa tattgagctg ttgctgagca accatcccga tgactgcctg tattgcgaaa
    32761 gcagcggcca ttgcgaactg caaaagtttg ccgaagacct ttacgttcgg gaaagacggt
    32821 tttacggcaa aaaaaacaag tataaaaccg atccgtccag cgcttccatc ttcagggatc
    32881 cggcaaagtg cattctttgc ggacgttgta tccgcgtttg tgaagaagtt cagaatattt
    32941 cggcattgga ttttataggc cgaggtaacg aaatgcaggt aggccctgcc tttaacaaag
    33001 gtatgaacct gtcgagctgt ataaactgcg gacaatgtat cctggcttgc cctacagggg
    33061 ccctgacgga aaagaaacac ccggcagaac ttcaggaaaa acttcataat ccggcaataa
    33121 aaactgtgat ccagtgttcg ccgaccttgt cggtaacact ggccgaagaa tttggccttc
    33181 gtcccggcaa agatatggaa ggcgtgatgt atgctgtttt gcgaaaaata ggtttcgatt
    33241 atgttttcga tacctccttt gccgccgacc tttacctggc cgaactggcc catgaattgc
    33301 tgaacaggat taaaaccggg gaaaaatttc ctctgttctc aagttgttgt ccggcctgga
    33361 tcaaatatgc agaacagttc aggccagccc tgctcgaaca aatatcatcc tgcaaatcgc
    33421 cgcagcagat gatgggcgct attataagca accattttac acaataccgg aagctgcaga
    33481 tcgaaaatat ctattcagta tcagcaatgc cgtgcatcgc aaaaaagttt gaatcacagc
    33541 gggaacaaat gacgcataaa ggcatcaccg atgtggatat ggtactgaca acacgcgagc
    33601 tggcccggct catacgcctc aacggtatcg acgttcagca ggttgagccc gagatggccg
    33661 atcagcctta ccgaagccgc agttcatcgg gaaaaatgct cgcagtgagc gggggactta
    33721 ccgaagcggt tatcagaacg cttcatacac tgattacggg caaggaactt cctctgagcc
    33781 ggattcccga attgcggtct tcaaaggaag taaaggagtt ctttatcaag atcggcgatt
    33841 ataagctggg ttttgccgtt atcaacggta ttggtaatgc tgcatcatac ctcgatgaaa
    33901 ttgaaaccgg gcgcgacgat attcattttg ttgaagtgat ggcctgtcca ggcggatgcg
    33961 ttggtggcgg cggtcagcct tccggccgaa acggggccgc cgtaaaatcc cgtgcaaagg
    34021 cgatctatga gattgatgat aaggaaccgg tgaaaacgcc ttacagaaat ccttcgataa
    34081 atgcgcttta tgaaagtttc ctgcacaacc cgggaagcga tgagtccctg aatttattat
    34141 atactgccta ccaaaaaagg gaagtattac tttagtacga cggttgccat tttgaaataa
    34201 atcaattcat ggaagacgcc accctgaata tcaaccagcc taatcccggg atgaccaaat
    34261 tggttttcac cgtcaaggaa aaatgcaagt tgtgttatac ctgcgtccgc gaatgcccgg
    34321 ctaaggccat acggatcacg ggcggacagg ctgaagtgat cacggaacgg tgcatagcct
    34381 gcggcaattg tgttaaagtt tgcagtcaga atgcaaaagt tttccggcgg gaaatagacc
    34441 ctgtgacaaa gctgatccaa tccggggcta aagtcatcgc aatggttgcc cctagctttc
    34501 cggccgaatt ttcagaagtt cctgaccatc gccttctagt tggaatgata cggcaacttg
    34561 gatttcgtca tgttctcgaa gtggcttttg gcgccgattt ggttgcgcgt aaatacaagg
    34621 aacagttaaa ctccggcata ccggcaattt catcggattg cccggccatc gtttccttta
    34681 ttgaacgata ttatccggaa cttgccggca accttgccga agttgtatcg ccgatggtgg
    34741 ccatggccag ggttgcccgc agtatttatg gcgttgattc aatcctggta tttatcgggc
    34801 cctgcctggc aaaaaaagcc gaatcaaaag aaatcgacaa tgcattgacg tttagggaat
    34861 tgagggagat gttcgatatc aaccggatca acttaaaaat ggttcttcct tccgaatttg
    34921 atcctccttt atcgggacgt ggcgcgatct tccctctcag tcacggcttg ctgaatacca
    34981 tggaagtcac cgaagacatc ctgagcgaaa atgtgcttgt tgccggcgga aaacccaatt
    35041 ttgctgatgc attgcgtgaa tttgaacaag ggaatctaaa gggccatcat attcaccttt
    35101 tgtgttgcga aggttgcatt atggggcccg gaatgtcacc atacctgaat acggcggcag
    35161 ccagtaaccg ttttgccaag aaggcggcaa ttatccggta cgccaatgct aaaatggcaa
    35221 ccatcaaccg cgaagaatgg caggaaaatg tggatgaata ctccaaactg gatctttcac
    35281 gtacattcaa gccgaacgat atgcgcgtgg taagaccttc aaacgaggaa atcaggggag
    35341 tgatgtaccg catggggaaa tatggaccgg gagattatct gaattgcggc gcatgcggct
    35401 atgattcatg cgaggagcat gccatcgcca tcatcagcgg gttagccgaa acggaaatgt
    35461 gccttccctt ttctatcgaa aggttgcata aatacatccg cgagctcaat atctcaaatg
    35521 aaaaactggc taccgttcag gaagccttac gccattcgga aaagctggcc ggcatggggc
    35581 agctctcggc cggaatagcg catgaactga acaatccgct cggtgtcatt accatgtaca
    35641 gcaacatact tcgcgaggaa gtgccggccg aagatcccat ccagaaagat cttgaactga
    35701 ttgttgagca agcggaccgc tgccggaaaa tcgtcggagg cttgctgaat tttgcccgta
    35761 agaaccaggt gaacattgct gaaacggacg tcgtgaagct ggccgaacac agtatcaatt
    35821 cggtgattgt gcctaccgat gttaccctcg gccttgaaaa ccatctgcgc gacagaacgg
    35881 ctatgctcga tttcgaccag atgacacagg tgctcaccaa cctgatcaag aattcaatag
    35941 aggctatgcc ggatggcggt aaaattacct taggcctgac cgacaccgaa gataagatca
    36001 tgatacgggt taccgataac ggcacaggta ttccggctga acacatggat aagctttata
    36061 cacctttttt tactacaaaa gcaatcggaa aagggaccgg cttgggattg ccgatcattt
    36121 acggcattgt aaaaatgcat aaaggcgata taaaagtaga atcaaatgcc gatccttcgg
    36181 caggcgccac aggcactacc tttaccctga tattcccgag gcagagggcc gttattcagt
    36241 aatcgcccgt gtaaatcata aacatcctac cattcaccga aaataaaaat cttatcatac
    36301 agcaatgaaa aaaagaatcc tgatcgttga tgatgacata gattacatct tccagatgaa
    36361 aatgaaagtt gaacagatgg gtttcgaaac cattaccgcc gaaagccaga aggaagctga
    36421 aaaaatcatc gaagaagtga aacctgatct ttgtatcctt gacctgatga tggaaactga
    36481 cgacagcggg tttatcctta gctataagat acgccggaaa tatcccgatg ttcccatcat
    36541 catcgccacg gcggttacag ccgagacagg gatgacgttc gacgtcatgg ccgaagaaaa
    36601 caaacaatgg atcaatgccg acctgttcct cgataaaggc atccgcgctg accagctcca
    36661 gcgtgagatt aaaaaactct taaaaatgta gctcatggca acattcaaga ttcttgtggt
    36721 tgatgacgag ctggggatcc gggagggtac aaaacgtatc cttcaaaatt tcaaggtcga
    36781 ctatcctttc atggatgaac agatcgattt tcagatcctg gaagcggcaa ccggcagcga
    36841 aggcatcgaa attatcgaca cccagcagcc tgacatcctt ctgctggata acaaacttcc
    36901 ggatatccag ggcgttgaag tgctggaata tgtcaagaac aaacattacg acatcattgt
    36961 cgtgatgatc acatcctatg catcgcttga gctggcggtt aaagccaccc gcgacggcgc
    37021 cttcgatttt atcccgaaac cttttacgcc ccaggagatc agggccagca tcgaaaatat
    37081 aaccaagcag cttttcctga agcgcatgac gcaatcgctc aacagcaccg gcaaacaaat
    37141 ccggttccag ttcctgtcgg tactgtcgca tgagctgaaa gcgccgctga atgccatcga
    37201 tggctatctt cacatgatcc gtgaaaagca gttcggtaac aacgtggatg cctataccga
    37261 aataatcgac cgttcaatgg aacggatcaa gggaatgcgc cagttaatcc tggacctgct
    37321 cgatcttaca aaaattgaaa cgggcaaacc tacccaaaag ctgctcccgg ttgatgttag
    37381 taaaattgtt caaatggcca tcgataccaa ccgtccatac gctatccaga aagacgtgga
    37441 tatttacctg aacaacaaat cggcagtcat gatgaaagcc gatgccggag agatagaaat
    37501 tattatgaac aatttgattt ctaatgcagt aaagtacaac agaaccggcg gccgggttga
    37561 ttgtttcatc gagaaattgc ctgaagctgt acggatttcg gtgtccgata cagggattgg
    37621 catgacggaa gaggaaacac agaaaatttt cgaagatttt gtccgcatca ggaaccagca
    37681 aacccgtgat attaccggca gcggacttgg cctttcgatc gttaaaaaaa tagtcgacat
    37741 gtacaaaggg gctgtcgatg ttcaaagtac cccgggcaag ggaactactt ttaccattac
    37801 actgcctgtc cctgagaatt aaaaaggttg ccggctgttg gccaatcccg gtagaaaaca
    37861 ttagccgtgc cggcggcagg ccagtacctt ttaccctgaa agaatgcaat tattaatgca
    37921 aataatatga aacagttacc tgataagacc gtcgaacgac tgagccaata tcgacgaaca
    37981 cttataaata ttcttgccga gaacaaacat cacatatttt cccatgaact tgcagctttg
    38041 ctccatatca caccggtaca ggtgaggagg gatatcatgc tcatcggata ttcgggaacc
    38101 ttgcggcagg gttatgatgt gaaggaactc atcgaaatta tcggaaaaat catcgatagc
    38161 cgcgaaggac aacgcgttgc cgttgtcggc atcggaaacc tgggcaggtc aattatcggc
    38221 tatttcagcg ggaaacgtac aaaacttacc atagtggccg gattcgacat caatccggaa
    38281 aaagtcgacc gcgtgtatgg aggagtctgg tgttatgatt ttagccggct tgatgaaatc
    38341 attaaaaaag aaaatatcaa tatcggggta attacagtcc cggcttcgga agctgccgct
    38401 gtagcggatg cactcgtgat ggccggcgtt aaaggcatcc tgaatttcac gcccaggccg
    38461 ctgaatgttc cgtcgagtgt gtatcttgaa gagtatgaca tcattacctc gctcgaaaaa
    38521 atcgcttatt tcgttaaaaa gatgtagcaa caatctgatt tgacgccggt cctgatatga
    38581 tgaagaatgc tgtgcaaatc atttcagcct gcggtaaaca taaaacctga agaactccgt
    38641 atgcgaattt tccatggctt tgatgaactt accaatatcc cgaacccggt ggcaacaacg
    38701 ggttcattcg acggagtgca cgtcggccat aaagcaatca tcaacagggt taacaggctg
    38761 gctcaggaaa tcgggggcga aagcgtactc atcacttttc acccgcatcc ccgtaaagtc
    38821 ctgttccctg aaactgccgg gaaagacctg ttcctgatca attcacagcg cgagaagatt
    38881 gacctgctca aagaagcagg gctgaacaac ctggtagtgg ttgaatttac gaaggaattt
    38941 tcagagattt cctcggtcga tttcatccgc aatcttctgg ttgccaagct tcatgcccgg
    39001 aagatcgtaa tcggtttcaa ccaccagttt ggccataacc gcgaaggaaa ctttgatttc
    39061 ctcaacgaac tcgggaaata ctatggtttt gatgttgaag aaattccgga gcaggatatc
    39121 cataatgaaa ccgtaagttc cacgcttatc cggaaggcat tgcaggaagg ccgcattcaa
    39181 cgtgccaatg catacctcga tcatcattac atgattatcg gaaaagccgt caaaggaagt
    39241 acactgatga gcgacctcga atacccgaca ttttatactg cgatagagga agaatgcaag
    39301 ctggtgcctc ccgacggtgt atatgcaata aggatagaaa atgccgggaa gtcttccaaa
    39361 gccattgtaa acatccggaa taaccggtac ggccccgggc ggaacttcag cgatctgacc
    39421 attgaagtat tccctttcga acagcccggt atcgtgaacg ggaaggatgt caggttgttt
    39481 tttgccaaaa ggattcgcga tgaactgagt ttcagcaatc ctgttgattt aaagcggcaa
    39541 ctggaaaaag acagggaact ggtcggggaa cttatcttct gaagaatcgg gaacatctcg
    39601 tcatatcgct tgtcaacctg taaacaaaat gttacgtgtc tgaaaatctg aaaatgaaag
    39661 ttacttttct gggtaccggg acatccaccg gcgttcctgt gatagcctgc aactgcgggg
    39721 tctgcacgag ccatgatgcc cgcgacaggc gataccgcac atcggcgttg ctgaccatcg
    39781 gggatacaaa catcgtgatc gactgcggcc ctgatttccg aaaccagatg ctgaagaaca
    39841 gggtggagaa tatcgatgcc gttttgttca cccatgctca ccgtgatcat attgcgggac
    39901 tcgacgacat cagggctttc aactatattc tcaataaaat catcaacatc tacggaacca
    39961 gggataccct tgaatccatc aaaatgcagt tcccgtatat tttttcaggg ggctttttcc
    40021 gcgttccgca gattacattg aataccatcg atgacaagcc ttttaccatc agcggcatcg
    40081 aatttaatcc tatctgtgtg atgcaccagg acctgccggt gaccggatac cgtatcggcg
    40141 attttagcta tattactgat gccaattata ttgaggccaa agaactggat aaaatgaaag
    40201 ggtcgaaagt gattgtgctg aacgccctgc gcaattcccg gcatgtttcc cactactgcc
    40261 tgagcgaagc gcttgaaatc cttgacattt taaagccgga acgtgcttac ctgacccata
    40321 tcagccattt tctggggaaa catgaagaag ttgaactgaa gctgccgcct aatgttaagc
    40381 tggcctatga caacctagtc atcgaagttt gatcaaaacg ctgtaaaaat aatagtttgc
    40441 aaatgatctc cgggttccgg gagcaggatt cggaataaat taagtattat aataccgaaa
    40501 ttgattcagt tgttgtaaat ttgtgagtaa taggagatgc aaccatgaaa tttacacctg
    40561 aaaaatgccg cattcccgat caacgaatga aaccgttcat tgatgcggat gaaatatggg
    40621 aatacctcaa cactgtaaac cccgacaggc agcaggtaaa agacgtcatt gcaaagtcgc
    40681 tcgacaaaaa gcgcctttcg atggcggaaa cggcggtgct gatcaatgcc accgatcccg
    40741 acctggtcgg ggaaattaaa gaaggcgccc gtaccctgaa agagcgggtt tacggaaacc
    40801 gcattgtact gtttgcgccg ctgtatatcg ggaacaagtg cagcaacaat tgcagttatt
    40861 gcggcttcaa atcttcaaat acggctgcta ttcgcaaaac ccttgacgac caggaactga
    40921 tccgcgaagt ggaagccctg gaagataacg gccagaaacg acttatcctg gtttacgggg
    40981 aacatcgtga ttacagtccc gaatttatcg cgcataccgt aaatgtggtg tacggcgtaa
    41041 aaaaaggcca tggcgaaatc cgtcgtgtca acataaatgc agcgcctctt gaaattgaag
    41101 gattccggac cgtgaaagca gcaggaatag gcacttacca gattttccag gaaacatacc
    41161 atcctgaagt atatttacat tatcaccttg gcggtgcaaa acgtaattac gagtatcgcc
    41221 ttacttctct cgaccgcgcc caggaagccg gtatcgatga tgtgggcatc ggggcgttac
    41281 tggggcttta cgactggcgt tttgaaacga tggggctgat ccgtcataca aatcaccttg
    41341 aagcctgtta taatgtcggt ccgcatacta tttcgtttcc ccgcatccag gaagcttcgg
    41401 ccatcaattt cgacggcaag tacaaggtgt cggattccga tttttcaaag atggtggcga
    41461 ttttgcgcct tgctgttcct tataccggga tgatcctcac tgcccgcgaa aatcctgaat
    41521 taaggcgcga agtgatgcat ttcggcgtat cgcagattga tggcgggacc aaacttgaaa
    41581 tcggcgctta ttccgacagc ataaacgaaa agcaggatct gaaccgtgaa cagtttcata
    41641 tcggcgacag ccgttcgctg aatgaaatta tcgacgaact gatcgacacg ggctatctgc
    41701 cttcattctg tacggcttgc tatcgccttg gacgtaccgg cgaacatttc atggagttct
    41761 cggtgcccgg gttcattaaa agatactgct cgcccaacgc catccttacc ctgaccgagt
    41821 acctggagga ttacgcccct gccgaaacca ggcggaaagg atacctcgcc atagagaaaa
    41881 acctccttga actcgaagct gccgccgaaa aaatagatgt tcccgaaatc cgccgccgta
    41941 tcgaaaagat cagggcagga cagagggatc tctattttta agaaggtttc ggacgtaatg
    42001 atttatggcg gattaccggt tcgttgtctg ttactcttcc tctgccggct gacaacattt
    42061 atctgatgaa cattaaccaa taactattat ctttacctat ggctttgtca gcgaacttgt
    42121 tttctcctgg cttttggcct gccgtcttac ccacaacccc gcccaggcag gtaaactttt
    42181 agcctttagc ttttagcttt tagcctgccg tcttacccac cacctcaccc aggcaggtag
    42241 acttgccatc ttgcgcacag ccagcccagg caggcaggcc attagctcct tgctccatgc
    42301 tccttgccca ctccgttctc cgttctccgt tctccgtttt ctgttctccc tgctccctgc
    42361 tctttgacct ttgacctttg acctttgacc tttaaccttt aacctttaac ttttaacctt
    42421 ctcctatgtc ccaaatccga attatcggct tattcatagc cgaccgcatc aaagaagccg
    42481 ggctgaccca gcatgtcctg gcaaaacact cccgcatcat ccgtgcccgg ctgggattcc
    42541 acgaactcaa cgaggatata tgcagccgga acggcatcat cctgctgaac cttacaggta
    42601 caaccgaaga acaggatcgg ttgctgaacg acctgaagga aatcagcggc atcgaagtcc
    42661 gcgagatgac gttcagttcc gattccgtga atgtaagcaa ctgcggctga aatgccggaa
    42721 caggtctaac tacaaaaaaa atatccagat atgaaagaaa tacggatact tggcatttat
    42781 gtaaaccgca gcaaagacga tacgcagcgg atacaggaaa ttttaacgcg ttatggctgt
    42841 tccatccgta ccaggcttgg gctgcacgat accgacgagg aatattatga cgaccgcggg
    42901 ctgattctgc ttgaattgta cggaaatgat gaagaagccg tacgtctcga gaacgaattg
    42961 ctggagattg attacgtgga agttcagaaa atgacttttc agaaaaagta gagaacgcga
    43021 tagcaggctt tcggctattt tacgccgctt cctaggatag tgatggagcc tttctttttc
    43081 gatgtcttgt aataaccaac cagttttaaa acataatagt atacgccgtc gggcaggccc
    43141 ttggcagtcc agtcgccatg atagtcgtcg gtggcataca cacggcgtcc ccatcggtcg
    43201 aaaacataga gttcgagtct tttatactca ttgctgatga tccagtcgtc atgctgtttg
    43261 tctttattta ctatcatgaa agcgtcattc ataccgtcgc tgttgggggt gatcacattc
    43321 ggtatgtcaa ttgtagcttc cttgataggc aacttcatct ggatcagact atcgcacccg
    43381 ttttcgttct ttaccagcag ggagaggttg aaatacccgt ccttgttctg gtcggcagtg
    43441 gcaaaggtat gtgtgggatt gaatgaccgg gcggtatccc cgtcgccgaa atcccataaa
    43501 ggaacttcgg tgacatgagt tggtgatatt tcatcgaagc ggaacgagag ggttgggttc
    43561 tggaggtaaa tgattgaatc aggctcaatg atcaatttga cattagcgcc gggcatgcca
    43621 tttacagtaa acatcgaatc gaattcgcag ccattggcat cggtaatcag catttcgttg
    43681 ctgccgctac acaggtttgt ctcaatcgta tcaaaccggc cggggctgcc gttccaaagg
    43741 taggtatagg gcggtttccc gccggatgcg ttgaccatga agttggcatc gcaggaattg
    43801 gcgcaggtgg ggtcaatttc ggtaagcacc atagagactt tcccgaagac ggtaacagat
    43861 cctgtgaagt attcatcggt cagcaggctg ccgtcgggca tgtaataaaa acagttgagt
    43921 tcgtcaaaag ccaatggtgt tgtccccgaa gaagtgaaaa agatcagttc ggctaacgtg
    43981 tcgttgatga tgcttgccgc ctgcgatttg cggacccatg agatgattac ttctccggcc
    44041 gaacttttta tattgaattt tccgcccgtc agttgcgtat ttaccgcgcg gtatgttata
    44101 tagttcagta ccagcgggtc gaaagtgagc gtgagctgaa gcgaatcaac gccaatcata
    44161 ttctttaccg taaccggaat ggcataatta atgcccgggc aaacactctc ggtggccgtg
    44221 ccggtttcaa ccggcaacgt ctgtgattgt accgacaata caggggtaaa agtgaaaaac
    44281 agaaacgaaa taatcaggag cttcgaaagc ttgccgcgta aagaatacat ccggttaccg
    44341 ataatcatga gttatgtagc aaaaatactt gaaaaattga ttgccgcctt attttaacgc
    44401 cgggattgtc gttttcgtgt ttatccgtgt aaaaatcggc ctttctgtta ccgagggcaa
    44461 ccagcaggat attcatgatt ttccgaaacc cggatgcagg gcgccatctt ccggtatcag
    44521 ccaatctgtt tcagcgtaat gtttttaccc cggaaattca ccgtgtcgcc ttctttttta
    44581 ccctgtaatg cttcgtataa agggacgccg gtcgaaatgg cgtaaaaagt gccgtcggta
    44641 gtttctactt tgcccagccc tacggcgata aaatagctga agtcgtttgt cgtcactatc
    44701 gcaccaaacc ctacttcccg gtatgagcgg cttaaatcga ttttgtccag catcttcaat
    44761 tcctgcaact ctttagcaag ctgttgggcg agcatgtcgc gccggcgcat cagttgtgtc
    44821 ctgaaaggat catagcggtc cttgggcgcg ccgtactcgt tcgactgctg ttgaaggtca
    44881 tccatcgcct gttgcagatt gatgcccgaa tcggtaagct tctgccggca tatttcaatc
    44941 aggcgctttt taagggctgt gtccatggta aagtattgta tttcgtgccg gcagtttgcc
    45001 cgtcttccgg atcattccgc cggtaattat tcataggttg cgtgtttttc tggttatggc
    45061 agggaaaatc cgataacaga aaagtgtggc agggaaccac aaaccaataa tacgaaaaat
    45121 ttccgaacag tggattattc gcgcaagggg aaattgttct ccctgctatg gatggttatg
    45181 gctgcatttg atgagtttcc tttgttctgt tatcttctca ttgccggaga gttttaacag
    45241 ataaatcccg tcggggaggc ctgagatatc aacctgcacg ttagcgccgc ttacttcgcc
    45301 gctttggatt ttccgtccgc tgaaatcgta tatcatgtat ggataggaac caggtttaag
    45361 cccgctgacc gtgaacttta ccgtagcggg gttggggaaa atattccagg attgtatttc
    45421 aggggaagga atacctgtct ctgtcgtgtt gacggctttt ctgagcgtgt ctttcaggct
    45481 gcagtgcgat gcaatcagcg aaacggtata tatgccaggt actgcataat tatgtacagg
    45541 atttatttcc gccgatgtat cgccatctcc gaaatcccac aggtattcgt gtgcgttcag
    45601 ggaggtattg ctgaatgcga cctggttgct gccgttgatg gtacatgaga atccggctga
    45661 aggatcatat tccccgatat gccatttata aagactgtcg tatactactg attttacaat
    45721 gttcctgatg gtggcggcat cggcagccgg aagcgaggcg tcgtaagtga tgagttccgg
    45781 gtttttccgg aagagtgtgg tataaaagca gcaggctgtg gcataggttc ccgcaaccga
    45841 aggatggctt ccgtcggact ggtacagttc gatggaggga tagtattgcc ggatctcatg
    45901 ccatacggcg cctgccggtg acagaatggc ttcgttgctg tcggccatca ttctgtaacg
    45961 caggttcaac aggctgtcca tcccttcgta ggtacaaacg ggcggccacc agttacaatt
    46021 ctcatcatct ccgttttttc ggccccacgt catgtaaaat acggtttcgc tgcaagcgtt
    46081 atacgcattt ataatgctgt ccagatagct tgcatacggg aaaacttctg tcattacctg
    46141 acttagcgga aacgacggaa gctggctttg ttcctgcagt acgacgtaat ccaggtttcc
    46201 gctcatgatt ttggcaaggg aggcagcgtt ggtggaatgt tcatggagcg tatatccgcc
    46261 cggcgtatta ctgtcgaaaa acagggtgtc gccggttgaa gccgctatat cggaaatcat
    46321 ccggggaaga ttattcgcat aagtatagct gttgccgagg aagagacatc ttttagccgt
    46381 gccctggccg aaggccggag ccgaaagcag aagcaccatc aggcaatatg gcaggcggtg
    46441 gtgtgcggaa tgtgttttta tcccccgaaa cagaagcccg aaaagggctg caaggctcaa
    46501 ccttaccatg acgctgcgaa tggtttattg aatgaggaaa gcctgaatcc aatcggtgaa
    46561 tatccgttct ttgtttataa aaatcaggga tatgccaatc atatcgtaaa aaccatgaac
    46621 aaacatattc agtatcagat tgtttctgtt tttgtaaaaa atcaacgaca ggcataagct
    46681 taccaatcct gtggatatca tcccggacat accctgatac aggtgagcca tgccaaacag
    46741 aatggacatt gcgattgctg aaaagagcca tcctttattg gtatcgccaa gaattctggc
    46801 aaattgcttc atgaagaatc cccggtataa aaactcctct ccaatgccgg ctacaaccca
    46861 catgaaaagt ataaaaacca gaaaattgat ataattcccc cggatgccat caaaactgct
    46921 taaatcaatt gcgccaaatt tgagttctat cagaggctgg atcaaaatgt ctactataat
    46981 aaatatggaa atagctaatg caatggctac taaaattgat ttgatccacc ggggtctgct
    47041 gataccaaac tcgatccatt taaatcttcc tgaccagaga accatcagcg ctgcaatcat
    47101 gccaaaaaaa tatcccagtc cacctgtaaa atgggtaagg acggcgctga ttaatataac
    47161 cagggcaatt acgattggct tcgtcagaaa atcgtgaatt cgttctctca ttctgaaagt
    47221 gttatttacg ccagggtgat atacaaatgg tccgtcatgg atatcgggtc ggggaaaatt
    47281 gtatcaggaa acgggaatcc agcctggaga atgtaagcta tatcttgctt tattggtaaa
    47341 tttcttattc ccctgtcagt tcttggataa ttccgacggc gtcggaaatg tatttttcgc
    47401 aacgcatctc gtataaatta ttctgtttcg caaaaagcct tccttcttcc gacatcaggt
    47461 cgcaattcaa caggctttta caatcggtgg agccgtgtaa tgccataaac ttttcgttga
    47521 actgcctgat catggcataa gttttatctt tcctttcatt gttgtcggaa tactttttac
    47581 agttatagat gccaatggcc atgaacgatc cggtgacggc gccgcaggtt tgctggagcc
    47641 tgcccattcc tcctccgaaa ccgcaggaaa tgcataaagc caggccggcg tcaaatttca
    47701 tatcttccga gtaggccgca agtaccgact gtgcgcaatt atatcccgac ctgaataatt
    47761 tgatggcttt atcgtgttcc ggtttcataa ttcctttttt atttgccaaa gatagggatt
    47821 ccccgaaaac gcttgtcttt tgcagaaggt tgctagccgc tgtagaatgc aaatcaggcg
    47881 gcgctgtttc cctgacctgc tacgatgcta aaaatgatat ttgctgacga agctatcgat
    47941 ggctttatca aggtcttccg tgttttcgaa aaacggcata tgaccgacat cgctgcccca
    48001 cagcagcatg ttggggaaag ctatctcttt gtaatgggaa ggtcccacag cccagtcggt
    48061 tttcccgtaa aagaaaagga ccggctgagt tacctgcggc gtcagtaagc aaaagtttgt
    48121 ccagtaatcg gcaatctcca tgcttttatt gctaaaatcg ctgttccact caggaaaatt
    48181 gagataggtg tcgttcattt tatttccgtt ttcttccgaa gcgaagaata atttccacat
    48241 ggtgcctttt tcgttcagga cttgcgaaat agcccccatc ctttcgaata tatttacatt
    48301 ggtatccaga catatcggag gcgtatcttg tccggcaagt tcaatggcct tgggcagcca
    48361 gctttttttg aaacaatcgt tcatggaaag gcagcaattg atgaagatca gtccatcaat
    48421 agaacccggt ttaccggtta cgtatcccat ttgcagaatg ccgccgaaag aatgtcccaa
    48481 tgtcagccag tgttttattc ccagcgattc gcgaacttct tcaaaatcca tgatcattct
    48541 tgccatggaa aaattgccgt ctttcgggct ggttgaccgt ccaacgccgc gctggtcaag
    48601 gtatatcatc tgaaagtgtt gttccagcga atttccgtaa tacttttcga gccagtagct
    48661 tccggagccg ggaccgccat ggagatacaa acaggccggg ccgctgcctc gtacatctac
    48721 atatagtttg acgccgtcgg aagttgttat gtacttttct ccggcgttaa ccgtgaaaat
    48781 ggagatacac aggcaaaaca ttaatagaat ctcttttttc attttttttt attcttttaa
    48841 cgtttatgct atccataatg ttacaccgct gatacctgat tcttccctgt ttttgcctca
    48901 taaggagttt ttttttcttt ggtagacaac ctgttcccga attaataccc cggtttcgct
    48961 gcctgaagtc gtgcccggcg agatcccgcg ctataccgga agaggcgggg ctttggctga
    49021 attaagaatg gaagcaggat ttctttcaga ttccctgaac tgaagcccgc ccttgccgtg
    49081 attacgataa atataaacgc tgaaagcgta tttcaggcgc cgttacttta cttctgcggt
    49141 aattttttca gcaatatgca atgctgtgga gagggtgctg agatcggtat aaaggttgcc
    49201 atcggcgcct gaatgatgac ttcttccaat tggttttaac accgctgttt ctcccggttt
    49261 ttccatatct ataacagcgg cgtaaaccac ggcaggctga caggaagtct tgtattcggt
    49321 atcatccgga tacttgttgt ttgtccaata ttcattccag tcccatgtct ggttaatttc
    49381 catgaaaact tttacaaacc ttggggttcc cttatccgaa cgggtttcaa gggcaaaatt
    49441 cccttttggc gttgctccgg tataagcatc gggcaccgga ttctttttat caggaatgta
    49501 gttcgctatt ccttttgccg ctcctgcttt gtgcgaccag taaggcaaag atgccggcat
    49561 ctttacttgt ccgggcttcc attgccctcc tgatttgtcg ccgtaataaa aggtaccact
    49621 gccaaatgaa cgggtaataa acaaagtctg tataaaccgg cctgatgtat cctcgagcca
    49681 gatggcaaag gtgggatggt tgaatgaggg gccctttgtg aacgttaatt ccagagcctt
    49741 gccggaacca ttgatattcc cggtaatttt ctgaaatgtt tccgatccgg taatgccgga
    49801 ttcttttgag ggcctgcatg aggttgcaaa ggctacggca atgaaagcca tcaccagggt
    49861 atatgcagct atgttatgat ttgctttttt catggtacaa tcaataatta aagttctata
    49921 ataacgccca gtgtcgggag gatagttcct gattccgaag gcagatattt aagactatat
    49981 cgtgccggat cgttcgggtc gataaccgga ttgccgttgg catcgcgcag caacacaagc
    50041 acatccggat ttttcgattt gaagttgtag gcgttctgga catcgaaata cacgtttaac
    50101 gaccattttc tgaaataata ctctttatcg attctcaggt cgagttggtg aaaattggcc
    50161 aggcgttcag tattatagag attataatca gggtaaccct gcccgcttgc atcccaggct
    50221 tcgatgaggg acgaacggtt gtaatcatag ggagtatagg gtgctccgcc cacaaaacgc
    50281 cattttgcgc cgatgttcca gtttctgcca aggtctgctg atgccatgat attcaggata
    50341 tgccggttat cccatgccga agggataagg tcaccttttt tatcatcaaa ttcggaacgt
    50401 acaaaagtgt aggacagtat gacgtttaca tggttcataa accggccccg atagtagaat
    50461 tctgcaccaa aagcacggcc gccggaagtg ctctttactg cttcatcacc gtatacgcca
    50521 aaatcggcac ccttactcgc cagcgataca gaatccttta ccgaaaaggg atagttctcg
    50581 taaaacttca ggaaaccttc gagcgaaacg cgggagttat cattacgcct gtattcaagt
    50641 cccgcaacca ggtgatcggc cctgatataa gtaacaccgt tatccctgtt gacaagttta
    50701 ccggtttcat cgcgataacc aagcgttgta tacgatggca actgaaaaaa tctgccggca
    50761 ttggcgttca gaaaaacttt atccgtaata ccgtaagagg ctgaaatgcg cggtgagaac
    50821 tgattcagcg ggttcgacat gcctgcggaa taactgttgc catccagcct gattcctgcc
    50881 gaaagcgaaa gcctgttctc aaggtagctt ttactcacct ggccgaaagc gttgtaccgg
    50941 aagaaatcga ggaaggagtc atagtcgagg gaggtaagtg tatctttctc cgtcgggata
    51001 aatactttcc ggaaagtatt attcagatac tttatatatt caagacctgc gccggttaaa
    51061 atcttataac cgttgaggcg cgtcgtgttc tcgtagcgca gtttgttttc aatctcctcc
    51121 gactcatatt tcagtaccag accggcgttc ttatcattgt tctcaaattt tttagacctg
    51181 ttattcagca tattgcggct taacacccag gtgtcgtatg agttcttccg gtagtgcctg
    51241 tatacggcgc ctatggcata ggtccactgg tcgtaggaag gaagatagtt gaggatatat
    51301 tgctgctcct cagttggatt ctcaattccg gtattcagag tacttttatc tagcgatccg
    51361 atactgagta tagtaagttc gttttttaaa tcgaaacggg ttttaacctt aatcgtgtag
    51421 tcgttaaatg tgggcagaaa aggcagcccc agcgcactga agagaaactg aaggtacgaa
    51481 cgccgggccg aaaaaagcag cgtcgttttt ttgctcaacg gtccggaagc gctcgcggcc
    51541 atttcgctgg cgccaagggt ggcgcgataa atcattttct ctggattgcc gtcgatgagc
    51601 ctcatatcga gtatcgagct gagtgcatta ccccgcgagg cgggaaatgc gcccgtgtac
    51661 agatccacct cacgcagaaa gtcgctgttg ataatgccaa ccgaccctcc gctcgctccc
    51721 tgcgtaccga aatgattgag gttcgggatt tctattccat ccagaaggaa ccggttttcg
    51781 gaaggaccgc cgccacggac tatcaggtcg ttccggtacg aaactcccga agaaactccg
    51841 ggcagcgatt gtatgacctt tgaaatatca cggttggcgc ctgcgctttt ttcaatctca
    51901 gcgattccga tggttttcaa tgaaaccgga ctctcttcca ctttcttgaa aggtgaggat
    51961 atcacctcaa cctgggccaa ctgaaaaggt ttttccttca tggcaatgtc gataaaagtg
    52021 gtcctggcat tggtaacctg aagttcctcg gttatcacat tttcgaaccc taccgaagtg
    52081 gctgccagtt taaagaagcc gggtgcgacg gctgtaaaaa ggaaattacc gtcaagatca
    52141 ctcaccgagc caatgtttgt tcccaatatg atgatgttgg tgaatggaag cggttcgtta
    52201 ctcgaagcgt tgtaaacccg gcctttgatc accccggttt gcccaaagac gattaccgga
    52261 atgaatgcga gaaattgtat gagtatatgg ttgatacgca tggtctggct gaatttgagt
    52321 ctatatgtgt ttgtttaata tgcttattgg ctgattaaac aaaactgatg ctctaatgtt
    52381 cattcaggca ttgcaattac ccttaatact gttgattatg gttgtcgccg gagatttatg
    52441 taacggataa aaccattatc aatcgcgcca aaacgactat cacactccgg gatactttag
    52501 ttgccggcta ctccggcttt cgttttcaaa ataaagaaaa agattactca cggaaacagc
    52561 ttttttgggc catgtcacat ttttttttaa accgaatagg gaatggacta aataaaatca
    52621 gaaccgcgca aagtggaatc gctcactagt ccattttctg ctttgaacgg gatttcggcg
    52681 attcagaatg tcggatttga gtatgtgcac aggaaagagc cgttattagg atacttaagg
    52741 ttggccgtcg gatccgaatg gttcagggaa tgtagctgct gtaaaagctg gcggaggaag
    52801 cgttaaaatt caatccggac cgtaaccgtg tcctgcggcg ccagggaaac cgttgcgctg
    52861 ccggtgttgc tttccggctt atcagtaatt ttccagtgaa cattcaccgt agggttgtct
    52921 ttacaaagaa atccgttgat gagcgttgtg tcgtgcaact gcggatagag gcgatacggt
    52981 tcagcgacag cattgataaa gtttataaag accgaatcat aatcgttggg gatattaaca
    53041 acgtcaagct ttacataacc ggctattccg gtccggatcg tatcggaatt aaccggataa
    53101 taatctatga tttttgccag tgttgcctgc gccggatttt taacaagata tccgctctgg
    53161 cgggtcgtga ttttatagag aaatttatcg ttttccctga ctttaaaaca atatttcccg
    53221 ttttcatcgg tcaatgccga atcctgcacg aggccgatat acctgtccgg aaatccaatt
    53281 accaggccgt caaaacggtt tagtatcacc gttactcctg gtttcgggtt accggtgtcg
    53341 taatcgatca ccaggccccg gaacacagat tcattttttt catcctcttt gtggcatccg
    53401 cataaaagag tcaggagcag gaaggataag agcgcttttc gtggcatggc tttatggtta
    53461 aaaggtggct ggcagacgtt gattcgcgtg atgtttagaa cttccgcctc attgtggatc
    53521 aatttgtccg gcttgagttc tgtttctttt taaaaggatg atggacaata caacaaccca
    53581 gataaaatag gtgaaaatat taattcgctc ccaaatgccg gcataggggg taggttgatt
    53641 ttcggctatt tttgaaccct ccatgcctgc cagcgtaccg aaaaccagaa aaatcacaat
    53701 ggtaataaat gaaaaaatcc tgaaaccttt tccgaatgaa aatgcgccga aaattattgc
    53761 cggaaacatg catagaagca ctcctgttat ggtgatgacg atatgcatca ggtctgtcag
    53821 ggaagttccc attccgcgca tatgcatcgg ggcgaataag agtccgatga cgcccagcac
    53881 ttctttagaa attaccagga tggcaactat ccgaagcgtc ttttgatttc cggcagaaat
    53941 ccagatcccg gcgccaaaag caaataccag taggctgtat atgaggaatg ccggcactat
    54001 gacagaggaa gacggggctc cgatagcaaa taattcactg acggtttgcg aggcggagtc
    54061 atattcatcc catcggatag ctccgaggat agtggctgcg acatagagta ttgtagataa
    54121 gatgccgcaa attagaaggt tatcctttga aaaaaggttc aatactatgg attgctgagg
    54181 tgactttact tttttttctt tcataccggt ttttatagag ttggcggttg atgtccggtg
    54241 aacgggtcta ttataataaa ttttcagtag tgcccaaaga gtaaatccat atttctggtc
    54301 aaaagcttta cgggcttgag tagcaggccc ctctcctcga ggagaggggc atgggtgagg
    54361 tgcaacttgt gctgcacttc aataactcag gttcccgaag agtaaatcca tatttctggt
    54421 caaaagcttt acgggcttga gttgcaggcc cctctcctcg aggagagggg catgggtgag
    54481 gtgcaacttg tgctgcactt caataactca ggttcccgaa gagtaaatcc atatttctgg
    54541 tcaaaagctt tacgggcttg agttgcaggc ccctctcctc gaggagaggg gcatgggtga
    54601 ggtgcaactt gtgctgcact tcaataactc aggttcccga agagtaaatc catatttctg
    54661 gtcaaaagct ttacgggctt gagtagcagg cccctctcct tgaggagagg ggcatgggtg
    54721 aggtgcaact tgtgctgcac ttcaataact caggtt
//
LOCUS       DAOPYV010000012        52780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_487_length_52780_cov_11.535325, whole genome shotgun sequence.
ACCESSION   DAOPYV010000012 DAOPYV010000000
VERSION     DAOPYV010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 52780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52780
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_487_length_52780_cov_11.535325"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..1535
                     /locus_tag="PKI35_02675"
     CDS             27..1535
                     /locus_tag="PKI35_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43314.1"
                     /translation="MQTFRYLSAAIIILLSFTSQATTGYSSRCQKAHANILCLRFEEA
                     QTLIREEIKQNPENTQPYLLENYIDFLTVMIGEQEEDYARLKQQRNRRLAKLASGDAS
                     SPWYRYSQAMVYMQCGFARVKFRDFVTAGVELNQAYKLLEENNRRFPEFLPDKVCLGL
                     LHSLIGTVPDNYRWAVRTLKFRGTIRQGLSELQTAFHQSARNPEFSFLYPESAFLLAF
                     TSINLAGNHDATAGFIAEAGKQPLQQWIHDSPLMCYALANVYLKTGENDKAISLLKGR
                     VQGKGIYPFYYQDYILGVAKLNRLDKDANIPLLTFVANFKGLNYIKSAYQHIAWYYFI
                     NGDMTKYRLYVNRIPLRGNVQVDNDREAMRFAEKKAEQNIHLLKARLLFDGGYYERAM
                     SEIQNFEARAGNTPQREIVECTYRKGRIYDEWGKDDLAIPAYQSVINLGNNQPWYYAA
                     NASLQLGMIYENRHDEKMARYYFNKCLSLNFEEYHFSITHKAKAGLNRLDNK"
     gene            complement(1591..3063)
                     /locus_tag="PKI35_02680"
     CDS             complement(1591..3063)
                     /locus_tag="PKI35_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43315.1"
                     /translation="MKKILILVIFAAAALSGIAQNENDALRYSRLSYGGTARFLGLGG
                     AFGAVGADFSTLSQNPAGLGLYRKSEFTITPQLNTTSTEADFFGEKNSDNKYSFNLGN
                     VGLVMNWNIGGNRESSILKYLNFGFGMNRLANFNNRMIISGFNNQSSLLTQYMYEANN
                     DGNPLDYDHLNPFGSQLAYNTDVLVYDSASQDYWIDAPEGGIDQRKTVESWGTVNEMV
                     LSTGANFSDKLYLGATIGFPYIRYEEDSRLTETDTKGNIDYFKSFTKHDHFETRGTGV
                     NFKAGIIYRPLDFLRIGFAVHTPTYYNDMTDEYYSEMTSEFDQATGLSATSPNGSYNY
                     KINTPMRLMGNVAFIIGQSGLISADYEYLDYSTARLRNTSGADNDDFYYVNEAIRTKL
                     APAHNFRIGGEWRYEIVTLRGGFGYYGSPYVKDLNKNDGSKKTLSCGIGIHDQGYFVD
                     FAYTHAWMSDDYYLYDISLVPAASTAVQQNYFSVTLGFRY"
     gene            complement(3179..4825)
                     /locus_tag="PKI35_02685"
     CDS             complement(3179..4825)
                     /locus_tag="PKI35_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43316.1"
                     /translation="MKNYIALVAVSLIFITGCTTQYQARNYDDVYYSSKNKPLEIRTV
                     VEPQQVQPAEPQAANAETAQGYAEPANSDTANYSAGEGDTYINNNFYSDDYYDYAYAA
                     RLRRFHNPYCFNSYYSDYYTNMYWYDYDPFNWGTSIYLGYNFWYPSYYYYPSYYRHGW
                     YDWYYPNYGWYDPYPWYGGSGFYSGYNLGYWNGFYDGYWFGYHDNYYNSFDHNSSYYG
                     PRNGGGSSGSLGRNNHYRTFGERYEAGLASSRTRSAVETRRDGGGSGTISPSRNNSGA
                     SRDITAQPAAGSRNATVIDNNSRNSGIGSGTPATGDRQPRNAEMQRTATGSNEPAART
                     PQYRYVNPGAANASREVPKANAAEGDRNSSQQPEIIQQRQQRYANPSSRNAASPGTQG
                     VQRGTNVQPYSPPAYAKPRSSQEYTTPKYRNPRETDVTPSQRNNQGSGAPQNRSYSQP
                     SRPAYNQQAAPRQNKVYQAPAPRNDNYSAPSRNNSSSTPSRSSGTYSAPSRSSSTYSA
                     PSRSSGSYSSPSHSGSSSSSGSSRSSSSSSSGSSSSGHRR"
     gene            4962..6437
                     /gene="proS"
                     /locus_tag="PKI35_02690"
     CDS             4962..6437
                     /gene="proS"
                     /locus_tag="PKI35_02690"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNX43317.1"
                     /translation="MAKDLTTREENYSQWYNDLVIKADLADNSAVRGCMVIKPNGYAI
                     WEKMQAQLDKMFKESGHVNAYFPLFIPKSFFSREASHVEGFAKECAVVTHYRLKNSPD
                     GKGVVVDPEAKLEEELIVRPTSETIIWDTYRSWIQSYRDLPILVNQWANVVRWEMRTR
                     LFLRTAEFLWQEGHTAHATREEAIAETEKMLNIYAEFAEKWMAIPVLKGFKSPNERFA
                     GAVETYCIEALMQDGKALQAGTSHFLGQNFAKAFDVQYLNKDNQLDYVWATSWGVSTR
                     LMGALIMTHSDDHGLVIPPRLAPTQVVIVPIYKTDDQLAVISEKAQEIMKALRSKGIS
                     VKYDDRDTFKPGWKFNEHEFKGVPVRIAIGPRDLENGTVEVARRDTLEKSVYQITDLE
                     TKIEHLLENIQDNLYRKALVFREDNTYQADTWEEFIDILDNKGGFISAHWDGTPETEQ
                     KIKDETKATIRLIPLNNKQEAGKCIYTGKPSTQRVIFARAY"
     gene            6514..7266
                     /gene="kdsB"
                     /locus_tag="PKI35_02695"
     CDS             6514..7266
                     /gene="kdsB"
                     /locus_tag="PKI35_02695"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNX43318.1"
                     /translation="MKVLGVIPARYASTRFPGKPLTMIAGKSMINRVYEQCTQCRLLN
                     EVAVATDDQRIADHVKEFGGNVFLTSDKHRSGTERIGEVLQILKDENPSDHFDLVVNI
                     QGDEPLISPDQIVKTIGCFKDSNAEIATLIKPISDSKELFNPNVVKVVINKNGNALYF
                     SRQPIPFLRGVEPAEWLQHHGYFKHIGIYAFKTQILELIVRLTGSPLEKAESLEQLRW
                     IENGFSINTAITDFESVAIDTPEDLSKLTNIL"
     gene            7320..8366
                     /locus_tag="PKI35_02700"
     CDS             7320..8366
                     /locus_tag="PKI35_02700"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNX43319.1"
                     /translation="MIGPAISELLRGHECVIVPGLGGFLTSHASACIDETTGQFMPPR
                     KSVAFNASLSNNDGILAYHLACKNNISYTEALTRVKDWSYSILQSLRRGNAVEIGHVG
                     KLSLNTAGNIHFEPDAEANYLDESYGLPFFTKPALNMPVQVIQESASARKVKESIRNI
                     VPSTLKWAAVLAPLIGFFIWGSVNTPSIGNFVNNQSGIFAWSNTTPGKTSVITGNQAA
                     RPPVTADITSPAEILGPEACLISPASIPYEAMHSRGLTITEPCTDADTKSNAEKPSYF
                     IVGGAFREVKNAGRLVKALQQQGYPATIADTTHKGLYVVSIRSFSDREEAFRQLKTIR
                     ESGHPSAWVLKAKN"
     gene            8502..9176
                     /gene="trmB"
                     /locus_tag="PKI35_02705"
     CDS             8502..9176
                     /gene="trmB"
                     /locus_tag="PKI35_02705"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNX43320.1"
                     /translation="MAKRKLERFAENATFPHMFQLMWDDLQAGFGLRGRWRNDYFRNE
                     NPLILELGCGKGEYTVGLATHYPDKNYIGIDKKGARMWRGAKTTAEKQLPNVAFVRMQ
                     IEGIADVFAPGEVDEIWITFPEPHPNSPRTKKRFTSPQFIERYRQILKPEGIIHLKTD
                     SDLVFEYTHDIIKAGKHRLLYSTSDLYSNSSDEMVRDVVAVQTFYEQMWLSQGIPIKY
                     LCFTIN"
     gene            9192..9527
                     /locus_tag="PKI35_02710"
     CDS             9192..9527
                     /locus_tag="PKI35_02710"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNX43321.1"
                     /translation="MMQDESFFEKVYTVVMQIPAGRVTSYGAIARYLGTGRSARMVGW
                     AMNASPTCPMPVPAHRVVNRNGLLTGKHFFGGNKIMQQLLENEGAIIQDDLILNFKEL
                     FWDPAKELE"
     gene            complement(9548..10591)
                     /locus_tag="PKI35_02715"
     CDS             complement(9548..10591)
                     /locus_tag="PKI35_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNX43322.1"
                     /translation="MKQFKPAGILLILALLSPGCARQVMQTANPALKDGRYDSEFPYR
                     NCANELEEITRSVKKVNSYATYRTYVFGENSEITLRDLSGSDLFEKAKARIENNESVS
                     GTALVISYSGDKLALLTCAHVAVFPDTIIHWSEHSDLQSNRFIHSISLKTNQQLYIRD
                     IPEGGRIQVLASDSKNDIAILGRTVNQPALPVKVFSYPVGYSDQLEWGSFIYLVGYPL
                     GQLMVTKGIVSKPAEKNGNFMTDASFNEGFSGGIVMAVRDGVPNFELVGIGRAVSART
                     GYSLKPEKQIHEYAYNPAIPYNGNIFVHEEKEINYGITFVIPVNKVRQFYMDNRGRIL
                     GQGYNLDPFFSEK"
     gene            10741..11841
                     /locus_tag="PKI35_02720"
     CDS             10741..11841
                     /locus_tag="PKI35_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNX43323.1"
                     /translation="MEVSKDLILNALRNVQDPDLRKDIVTLGMVEDIVIEGKRINFKL
                     VLTTPACPMKGKMKNDCIEAIHSVVDPDAEVNVELISRTTSRRKAGENMLKDVKNIIA
                     VVSGKGGVGKSTVAANLAVSLARLGAKVGLLDADIYGPSVPLMFDLMDARPEVIEENG
                     QAIIRPIEKYGIKLLSIGFFVDPDKALVWRGPLASNYLSQLLTGSSWGELDYLVIDTP
                     PGTGDIHLTLVQNVPVTGIAIVTTPQEVALADARKAYGMFAAEGIKVPVLGIIENMAW
                     FTPEELPGNRYYIFGREGGKRYADEMGIPLLGQIPLVQGICESGDNGSPIAINDSSPV
                     AVAFRDLAETIARQVAIRNATLEATKVVEMKS"
     gene            11896..12135
                     /locus_tag="PKI35_02725"
     CDS             11896..12135
                     /locus_tag="PKI35_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNX43324.1"
                     /translation="MNAELESKVKNVIDQIRPYLQADGGDIRFLELTDENVVNVELQG
                     SCGSCPFSRMTLKNGVEEAVKRALPEVKSVEAVNI"
     gene            complement(12314..13966)
                     /locus_tag="PKI35_02730"
     CDS             complement(12314..13966)
                     /locus_tag="PKI35_02730"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNX43325.1"
                     /translation="MRIALAQLNYVIGDFEGNLAKITNAIRESRRQKADLVVFAELSL
                     CGYPPRDFLEFDDFINRCGLAIEKIAAECIGIAAIIGAPARNPDLKGKDLFNAAWLLA
                     DGEVKGTAFKTLLPNYDIFDEYRYFEPNRQFNVLEYNGIKIALTICEDIWNIGPDPLY
                     VATPMDEMIGLKPDLIINIAASPFNYDQENERGEVLAKNAGRYGLPLVYVNHVGAQTE
                     LLFDGLSRVLDASGDVAAMLPAFEEGLEFVDFKFVEGKLTPEPVVHPSHQTSSKTGLI
                     HDALVMGIGDYFRKMGFKKAILGLSGGIDSAVTLALAARALGPENVMAVLLPSEFSSQ
                     HSVDDARQLAENLGVRYELIGIRKGYDSVLETLQPYFKGLPFNVTEENIQARLRAVIL
                     MAMANKFGYILLNTSNKSEAAVGYGTLYGDMCGGLSVLGDVYKTEVYDLAGYINAEKE
                     IIPLNSITKPPSAELRPGQKDSDSLPDYSILDKILYQYIEKRIGPLEIIAMGFDEAIV
                     RRVLKMVNTNEWKRYQTAPILRVSPKAFGSGRRMPIVGKYLS"
     gene            complement(14046..14717)
                     /locus_tag="PKI35_02735"
     CDS             complement(14046..14717)
                     /locus_tag="PKI35_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX43326.1"
                     /translation="MNKKLLLLSLILVIGFTANAQYKKFKFGLKAAPQISWMRTNVEG
                     YSNDGAKLGFGWGFVTEMNFTENHSFATGFNVLFNGGKLQYPDISGTNAGTMKRTYKL
                     KAIELPLTLKMRTNPIGDFQYFGQIGLGTSFQLDAKSEDEFTYNSTVVTKEGNISNIA
                     FLRESLIIGLGAEYTLDAGTTFGAGLTFNNGFTDMLTGKNKVDGSLKEKGYINFLELN
                     AFVLF"
     gene            14901..15512
                     /locus_tag="PKI35_02740"
     CDS             14901..15512
                     /locus_tag="PKI35_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNX43327.1"
                     /translation="MKRVLFVAFLLSVAVSFATAQIPGFSIGPKVGYNSNRLVTNLDS
                     VTSTPEGAFQIGAFMRFGQKVYFQPEVNYIVKGGIINDVLDGEDRSQEIELKSITIPL
                     LLGVRPVSEGSFNIRLMAGPTLSYISEKHVKPLELLSSWPIHSTDDIDKMLWSFQVGG
                     GIDFWILTFDARYEVGISNIYTGDENLKVRNNLFNVSVGVKFL"
     gene            15569..16606
                     /locus_tag="PKI35_02745"
     CDS             15569..16606
                     /locus_tag="PKI35_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43328.1"
                     /translation="MGSQTDISFKSMRRFFFLLLFASVILFGSCHRNKQVFIPGPVAK
                     VPSQPVQIMRYDSALFSINSQNLKESLVPLAGRYYFFLGSDWSDTINLMNLSQFVNDP
                     NIRKLYEIAMQKYGNLSALKKDLSGAIARYRSYYPEKPCPLVYTYISGLNVEMPVLFT
                     DSAMAIGLDLFLGNDQVIYREAGIPEYKIARMMPEYILPECMKAIAGNVLRTDPGDQT
                     LLNQMITAGKICYFLDAMFPEMADNLKIGYTPGQLEWCKTNEENIWKFLIGNQLLFNS
                     DPAAASKLLADGPFTSGFAQESPGRLGVWMGWQIVRAYAANNKKLSLKQIMEATGSQA
                     ILEASEYKPGR"
     gene            17442..18980
                     /locus_tag="PKI35_02750"
     CDS             17442..18980
                     /locus_tag="PKI35_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNX43329.1"
                     /translation="MLVKTFGCATQGINATTITVEVNVDQGIQFAMVGLPDNAVKESH
                     QRIEAALVNIGYKFPHKKVTINMAPADIRKEGSSYDLTIAIGILAASEQLLVDDLDRY
                     VIMGELSLDGSLQPITGALPIAIEARDKGFKGFILPLENAREAAIVNDLEVYGLESLN
                     DVIGFLEGSLKQTPVEVNTREEFYNHLNEYEFDFADVKGQENIKRSLEIAAAGGHNVI
                     LIGPPGAGKTMLAKRMPSIMPPLNLHEALETTKIHSVAGKMGRNASLISVRPFRSPHH
                     TISDIALIGGGSNPQPGEISLAHNGVLFLDELPEFKRTVLEVLRQPLEDRMVTISRAK
                     STVTYPASFMLVASMNPCPCGYYNHPEKDCVCGPGVVQKYLNKISGPLLDRIDIHVEV
                     VPVPFRELNSERVSEKSEAVRERVVKARRVQEERFRESKGIYCNAQMSSKQLRQICQI
                     TEEGQVLLKNAMDKLSLSARAYDRILKVARTIADLAESPDIRTEHLAEAIHYRSLDRE
                     TWGG"
     gene            19092..19904
                     /locus_tag="PKI35_02755"
     CDS             19092..19904
                     /locus_tag="PKI35_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HNX43330.1"
                     /translation="MAKIIVKNTEITIISVDEKDYISITDMANAKESESRAADIIKNW
                     LRSRYTLEFLGTWEQINNPYFKVVEFDHFKSQAGLPNFVLSVSEWIEKTNSIGIIVKK
                     GRYGGTFAHKDIAFEFGSAISVPFKLYLINEFQRLKEEEQKQFGWSAKRELTRLNYHI
                     HTDAIRHNLIPQELSPAQISTVYANEADVLNVALFGITARQWRDANPGLKGNIRDYAT
                     INELICLSNMENLNAVFINEQMPQKERLIKLNRSAIQQMSVLQDAGNRKLLK"
     gene            20261..21109
                     /locus_tag="PKI35_02760"
     CDS             20261..21109
                     /locus_tag="PKI35_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNX43331.1"
                     /translation="MNLSHYQDQVNRLEKDVADFQKKIADESRKENDKNRQIDSVNKS
                     INKYTSESSLQSKLRQIRGYQNDIYNCKKKIAEYQKQIANKSVEIGRKKQDLRNAEEA
                     EQKKQQKDQLDFQRKLQQDVEQQKSQLNFLINQNYSSKTENFNDLDDKEYDFFISHAS
                     DDKDEIVRDLVDALKNNGFKVWYDEFELKIGDSLRKKIDNGLAKSKYGIVIISPSFLK
                     KKWPEYELNGMVAKEMNGHKVILPIWHKITKDEVLKFSPTLADKMALNTSIHSIGDIV
                     NSLKEL"
     gene            21264..21812
                     /locus_tag="PKI35_02765"
     CDS             21264..21812
                     /locus_tag="PKI35_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43332.1"
                     /translation="MTYKDDIKQKFENIDGHLWWPDTNKLSQLNGNADDFFDRTFPEG
                     KIAALFIYHQLMIEQMKMLIKYINYYIQVSLYPTVIQVNNFENESQFGSINEYFKYTI
                     EFKGKAQILKQAQILNRLRNNYGHSMTDKWLEDACDKELSNLKTDYYIFFKSWSNGIN
                     EIRKLVEKVKQSKDIKRLIELT"
     gene            21994..22905
                     /locus_tag="PKI35_02770"
     CDS             21994..22905
                     /locus_tag="PKI35_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNX43333.1"
                     /translation="MDFITLDFETATSQRHSPCEIGLTFIKNSQIVETKTWLIKPINN
                     EYDGFNIFIHGITPKDTADKPEFNIIWTELRPLIENQFLIAHNAGFDISVLRRTLELY
                     KLPFPSLHYCCSYIFSKKVWEGLPAYDLKTLCRVNDIPLVHHRAGADSRATAELALKA
                     FEIAGVISLDDFPEKLKTTIGQLYHGGYSPSETKRINKAKDLSKIVGDPAKHNAGSIF
                     YGRTVVFTGTLSSMIRTEAQQIIADIGGINGNSVTKDTDFLVVGQQDYRVVGEDGMSN
                     KQEKAVKLIEKGSTLEIISEDDFLKNL"
     gene            23045..24649
                     /locus_tag="PKI35_02775"
     CDS             23045..24649
                     /locus_tag="PKI35_02775"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX43334.1"
                     /translation="MKRTFLLLFIICSFSVVYGQKNIRSDKKKIKFSVNGAVQEWRIS
                     PEDNPDRLKVYCSHEKNTVIFQTNIDTALFYVSKNDTIKFNIILNHQDTAHTEIVGIK
                     DLPDKIPADEKLYWLSQIWSETKYNFVNIDRLKPDPDSLYRTFIPLALATKNDYEYYQ
                     VLKKFMANLHDGHSEVSDNGQFRPYTDYIPVSLQNFNNKVYITSVRKIPELDSTWVGA
                     EVIEIENIPTLKYLETNIFPYISASTEQHLWMQGMVKLPNGFKDQPFKGKIRKTDGGI
                     VNIELQHNGEATRTDHDQSWGPAINYPQDIVDLKWLDKDIALVSFNRFWPEEEAIKEF
                     DNVVDKLSKAKGVIIDLRKNGGGTTNVAWHLQKYLTKGNYFLNFASETRINDGVRKAN
                     GNWQEQYKDYFLNRAYRFERPDSVFVSDTLKRITCPTVILIGRFTFSAAEDFLVNIYE
                     TPERPILIGEETGGSTGSPLVVQGLPGGGYARICTRRICYPISEKRFVNSGVKPDIEV
                     KETIDDYMKNRDAALERAIKEIENAL"
     gene            24879..25256
                     /locus_tag="PKI35_02780"
     CDS             24879..25256
                     /locus_tag="PKI35_02780"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HNX43335.1"
                     /translation="MNYIKAFSQLERFVDVDKFNELEKLGLIKAFEYTFELGWETLQD
                     LLKEKGYYGIIGPKPVIEQSFQDGYITDGNGWMRMHKSRNLTSHTYDEETAEEIIRNI
                     RYEYFLLLKDLKGALEDELKKIQ"
     gene            25253..25588
                     /locus_tag="PKI35_02785"
     CDS             25253..25588
                     /locus_tag="PKI35_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNX43336.1"
                     /translation="MMTVTDKYGLNEIELENIISVFKENAKIDKVVLFGSRAIGNYST
                     GSDIDISISGRDLLLNDVLDLSIKLDEFDFPYKFDILIYNDIKEKALVEHIDRVGVVI
                     YSRYSFPDK"
     gene            25785..26384
                     /locus_tag="PKI35_02790"
     CDS             25785..26384
                     /locus_tag="PKI35_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43337.1"
                     /translation="MHFRLVMLPVILLFWASCTEEPEEKVRIPESIVAGQTSGNGIVY
                     TNPQPDDSFYFAGLASQEVFYDLNRDGNDDLVFSMTDYPDSVSGYYSASLRPVYPNQI
                     VRTPQLDYYLRPLNAGDTIGRNSYWHEGSAFMFYEGWDPGHHWSTGQWDTIRHAFAGV
                     KLITGTDTSYCWISLFHSKEPPHALVIRDYAYTIRYTEP"
     gene            complement(26543..27058)
                     /locus_tag="PKI35_02795"
     CDS             complement(26543..27058)
                     /locus_tag="PKI35_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43338.1"
                     /translation="MKKLVICFSLALVFIGLPSSAQITLLLEKPGTVTYYMYQKHDFI
                     SIRYKTKPEGFFDAGVITEITDSTVQINGNNRYRFSDITAVFRGRPLVRIFSNVAIIC
                     GAGFLGLTLVNGVIGSGGPALGPDVVSAAGSVIFAGLVASAFKNRRFILGENWRLRTV
                     DLSILKPDGQQ"
     gene            complement(27375..28382)
                     /locus_tag="PKI35_02800"
     CDS             complement(27375..28382)
                     /locus_tag="PKI35_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX43339.1"
                     /translation="MNQVISTQLMFFDVLKKRLPANLSLVHELSELLGISYDSAYRRL
                     RGEKDLSIEELRRLCIHYDISFDSLINIDHRHKVFNSIDLQGDSFNYYTKLSAIVKDV
                     SKKVLDCQYKDVIFCTKEISLFHFIPFPELLAFKLFFWSRIQYRLSERQDRRFSTADD
                     YSGLLGLNQEIYAYYLHIPKYEIWCNETLQCTLRQISYCWESGFFADVRDAIYLCELL
                     EQLYSHLKIQAEAGYNFPYGSEHSGIDNSFSVYYNDVFLVDSTILINTDHRMTAYLTY
                     NGLNLLATQDAEICLQIEQSLRNTMQSGVLISGTSAKERNHLFKGLIDDIRALKESLR
                     G"
     gene            28881..29321
                     /locus_tag="PKI35_02805"
     CDS             28881..29321
                     /locus_tag="PKI35_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43340.1"
                     /translation="MKKPIRILLMLATGIIAFSGCQKETDDLIPVNQNKAESAFNESS
                     QPLPGISWSNNFNTEASLLQSFYLVGSPAPVWVDEACGRQGLFDNNATSPTAGYGISK
                     VPIGNGGPVVIEAEVYLKITDANGYCVCPGLGITLKDYQPLVLW"
     gene            29352..29693
                     /locus_tag="PKI35_02810"
     CDS             29352..29693
                     /locus_tag="PKI35_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43341.1"
                     /translation="MYAGTQATWVPERCRGHAWIQAGYNNSSGKATTTGEYAMVADSY
                     TNSWHKIKIMITQDHFVRFYIDDVLIWSPGDKISSQQLQGKHLVLGFTSSGNVGKAYH
                     NYVKVTYPDYY"
     gene            29915..31090
                     /locus_tag="PKI35_02815"
     CDS             29915..31090
                     /locus_tag="PKI35_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX43342.1"
                     /translation="MAALRKFLPVVLVAIMALGFSCNNMSRKSMVIQGQFTGVPGKRI
                     VLYELFPDSLCILDSAATGEDGEFRFRIKSGDAGFYLLKLDDRNFITLSSGPGETIKL
                     SGDGNNLQHEYSVSGSAGSILYHDFLMFTNRNQSKTDSLGEIFITSRPKADFGEIKEQ
                     LDSAYYGIFGEQQQRVRNFLEANAGNLVSLLVINRRFGPNEVVTPATDLPLFIRIDSL
                     LSANYPGNKHAASFHRTLEAYRAQVSGTSLRNKAMLPGSPEPDLGLPDASGNIIRLTS
                     LSGKPHLIYFWGSWNAPSRKMNAGLIPIYNEFRKKGFEIYAIALDTQKEPWLKACRLD
                     KATWIHVNDMKGLDSPAARACGITGIPAAILIGADGLVKARNIQPDELKTWLMTFYK"
     gene            31109..31951
                     /locus_tag="PKI35_02820"
     CDS             31109..31951
                     /locus_tag="PKI35_02820"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNX43343.1"
                     /translation="MKLARPMTDSNKAIYFASDFHFGIPDRRGSLIRERKFIQWLDEV
                     KKDASAIYLMGDLFDFWYEFRTVVPKGYIRMLGKLGEMTDAGIEVHLFRGNHDLWAFR
                     YLEDELGILLHRAPMIVQLGNKKFFLAHGDGLGPGDHSYKFLKMVFECRFNQWLYRWI
                     HPDIGTRLGAYFSRRSRLTKMLKGEGDIKRKVPVEKEPMIIFSREMAQKDPSIDYFIF
                     GHVHFPDVVSISPKAQAVILGDWVSKFTYARFDGETVEMKQYCPRSDNSIPDTARPGL
                     PDYR"
     gene            32143..32655
                     /locus_tag="PKI35_02825"
     CDS             32143..32655
                     /locus_tag="PKI35_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43344.1"
                     /translation="MKTNLYLFIFLAVAASITWSGCGKEGPAGKDGIDGNANVIASQW
                     YKPATWAGKTGDWYFDISCNEITEDIVESGVILAYCSLPGDIYDAPVRPMPCYAIGAN
                     WDFLIPDYGQIEFTSDALYVPGTSDYYFRFILIPASNFLKTSSGTNVTADELRKMTYH
                     EVCTKYGIKE"
     gene            32790..34172
                     /locus_tag="PKI35_02830"
     CDS             32790..34172
                     /locus_tag="PKI35_02830"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNX43345.1"
                     /translation="MDTIKSYLEANKARFLQELFDLIRIPSISSESEHKNDMKLAAEY
                     ITESLLKAGADKAGVYPTTGHPVVYGEKITDPAKPTILVYGHYDVMPVDPVELWKTPP
                     FEPVIADGKIFARGAVDDKGQLFMQVKAFEYLVKTGQLPCNVKFMLEGEEEMGSPALA
                     KWLPENKEMLAADLILVSDTSMIALDIPSITVGLRGLAYMEVEVTGPDKDLHSGLYGG
                     AVANPANVLASLIASLHDENHHITIPGFYDDVTELSAEDRNDLARAPFNLEEYKRKID
                     ISEPLCEKGYTIQECTGIRPTLDVNGIWGGYTGEGSKTVLPAKASAKISMRLVPNQDP
                     HKIAQLFARHFERIAPPSVKIRVKEDHGGFPYVSPTSTRAYRAASKAYETTFGVKPVP
                     VRSGGSIPIITTFEKTLGIKSILMGFGLDTDAAHSPNENFQLECFYKGIETITWFYRY
                     FAGDLKQEIE"
     gene            complement(34220..35953)
                     /locus_tag="PKI35_02835"
     CDS             complement(34220..35953)
                     /locus_tag="PKI35_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNX43346.1"
                     /translation="MTIKTSFLSALLLLAFTGVSYGQSFTAVSDGFINVGRSSLAWGD
                     YDNDGDLDLLLSGDPVNGSYTMKIYRNDAGTFTDINANLTGVYNSAVSWGDFDGDGDL
                     DILATGRSASNSNTWLFRNKSGEFVPVNAGFPAVGSDGAVAWGDYDGDGDLDALIAGN
                     FSCRLFNNNGGVFTDVNAELPPINNGWVDWGDFDNDGDLDIFVMGDLGGVLVSDIYRN
                     DEDFFTAMGTGIMPLSGGTASWCDVDNDHDLDLLVNGFDEYLEPKTLIYQNLGGMQFF
                     CMIPGMVNSSLGTVAWGDYDNDGDADALITGQNPACGSLSSVIYRNDGGYMFTNISAG
                     LAGVERGCSTWGDYDNDGDLDIVLSGSSESGSPITALYRNNAGSNVFQENTPPLAPAG
                     LTSTVSGHQVLLAWNQATDDHTPTQALTYNLRIGTTPGGNEIIACMANSADGHRLISQ
                     PGNVSNDTSWVVNLPDGNYYWSIQALDQAFLGSQFAGEQQFTVLNAGILKNENPAVCH
                     FQNPFSDQLSIRSEGKFRIQIRDSFGRIVYTGDEPEFHHEIETACWPSGVYLLNISGE
                     KGNYMMKAIRK"
     gene            complement(36109..37440)
                     /locus_tag="PKI35_02840"
     CDS             complement(36109..37440)
                     /locus_tag="PKI35_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43347.1"
                     /translation="MKKLIFALMLMMAATLSVEAHRYYPTHVTISFQTFYDELSPYGE
                     WIYAPDYGYVWRPYFDRPDAFRPYSSGGNWALTEYGWTWISDYRWGWATFHYGRWYFD
                     DFLGWMWIPGFEWAPAWVTWGSYNDYYGWAPMGPDIYVSVNFNWFAPDPWWTFVPRRH
                     FCSHHWRNYIYDRPVQINHITHITNVYYTDNPGMHRNDNWFYGPRVSDVERYGNTRVR
                     TMRVVDTDRPDNLRTRDGQVDMYRPSVDRNRGESRPGEYRNAENLRSGTRITPQNPRT
                     NDPGVNHRREAGAEPQGATNSKRNYDAIQTRPGRVEPGNNVKNEQKRNAQPVNSERGK
                     ESTDRKRTEKASNDNGPSAAGNREPAIKSDNKRSSEPSREAVKRESSDRRNAEVKRSA
                     SEERSKEKAGRSTESSKVERKQAKSDTGNKSERSRSSDKTSSRSSRSSRNR"
     gene            complement(37694..39787)
                     /locus_tag="PKI35_02845"
     CDS             complement(37694..39787)
                     /locus_tag="PKI35_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNX43348.1"
                     /translation="MSKLTLQAVVAVLFLAVTAGIKPVTAQILEPVKWTYKTNRVNDT
                     IAELNFVATIEPGWHLYSQKKYPVGEGPLPTEFKFEKKTGAVLQGSVKEPKGHEIYDE
                     MFGQKIRSYDMKAVFTQRVKITGNKPAAISGILYYQTCDDKQCISDEKTFSFTLEGAK
                     NAKAGSDQAVETTTALPDTSAKQTVAAAPDSVAKADAAAGPNSDEFTGKNRSLWWFFI
                     QAFAWGLLAILTPCVFPMIPMTVSYFMKSGSKGKVQAVVYGLSIIAIYVIFGVLISIL
                     FGADFGNWLSTHWIPNVLFFIIFVVFAASFFGYFDITMPSWMVNKSVQNEEKGGVVGT
                     IFMALTLVLVSFSCTGPIVGTVVVQAVGGEVLKPVIGMLGFSLAFAIPFTLFAFFPQW
                     LNKMPKSGGWLNSVKVVLGFIELAFALKFLNVPDQTYHWGILDREVYLGLWIVIFAML
                     GFYLLGKIRFPHDSDYPVVKSFPRLVLIIATFAFVVYLIPGLWGAPLKGISGWLPPMD
                     TQDFDVTAIVRDNSGTGGGGSVAAGNLAETSKYANTELKLPHGLKGYFDYDEALKVSK
                     ELKKPVFVDFTGHGCTNCREMENRVWSDPQVLKRLRENFVLVALYVDDKVIEMPKEDW
                     YKNKEGTEVKLLGRKNTEIQRERFGTNSQPYYVILDGDGNMLVSPRAYDLDINEFIKF
                     LDNGVEAYNKKAAGK"
     gene            complement(39890..41233)
                     /gene="tilS"
                     /locus_tag="PKI35_02850"
     CDS             complement(39890..41233)
                     /gene="tilS"
                     /locus_tag="PKI35_02850"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNX43349.1"
                     /translation="MFKRFLHFIEAESLFNAKDNILLAVSGGIDSMVLCRLMFEAGFK
                     FGIAHCNFNLRGDESDADEKFVETAAAGYKVPFFAIRFDTKQVAHSQGISIQMAARDL
                     RYAWFEEICREKGYDYIATAHHLNDQAETFFINLVRGTGIAGLHGILPLQGRTVRPLL
                     FATRGEILDFAHSQNIVWREDSSNASTKYLRNQVRWELLPVFEKLNPGFVQNLDDTIR
                     RLRGSESVYKQKLAEGISSLVEHEKDRDRIPIGSLRKLEPAETWLFEALRPYGFSETV
                     TKEIARSLGGTERKAFFSPVYRLTVERDFLVIEETAKIADMGDIEFPVEQHGNEFIST
                     APVPVDFTCAKAEGFKLPHDKEIACFDYDRLEFPLTLRHWREGDRMVPFGMKGTKKLS
                     DLFIDMKLPQSDKSKRWLLCSGSNIIWVLGVRTDDRFKITKRTQNIFIAVINDQS"
     gene            41395..41727
                     /locus_tag="PKI35_02855"
     CDS             41395..41727
                     /locus_tag="PKI35_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HNX43350.1"
                     /translation="MKIAENGQRLTIYIGERDMQGDITLCETIVLKARELNMAGATVI
                     HGFMGYGSSCRLHSSLVTHFSNNLPLVIEIIDKPSKISLILPFLEEVLDEGLYTVEDV
                     KAVWCKKG"
     gene            41953..42636
                     /locus_tag="PKI35_02860"
     CDS             41953..42636
                     /locus_tag="PKI35_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43351.1"
                     /translation="MRNILLFLTSLFVLSGPLCAQDNEENYKSLEFHTMPFTLLDLSP
                     RFRLGAEYRITQRFACVLDCGFGNNDLNGWVLRETVWKNDYSFIEVRPELKYYFPKAD
                     DITSFYVATELFYIRTNSILKDGYYHPENQPFVICYELATFNRQKAGLHIKGGVTLSF
                     SRFDFDFYGGLGIASREINYSHITNPETEEEDIFFEEGFNNSYKYEGGSILPHLTLGC
                     KIGMVLWKD"
     gene            complement(42757..43800)
                     /locus_tag="PKI35_02865"
     CDS             complement(42757..43800)
                     /locus_tag="PKI35_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNX43352.1"
                     /translation="MIEFPEKTIERSSVELSKEDFVSDQMVKWCPGCGSHAILAAVAN
                     VFPKIGYRKENFMMVSGIGCSSRFPYYVNTYGIHGIHGRANAIASGVKIANPRLSVWI
                     TTGDGDSMAIGGNHFIHIIRRNIDVNIILFNNQIYGLTKGQYSPTTPMGSITKTSPQG
                     TIEHPFNPGELVLGSQGTFFARVVDNNVKMMTEVMFEAAKHDGTSVIEVLQNCVIFAD
                     KTHAAVTDKEIRDDVQLHLKNGEPMIFGKNRDKGIRLKGTRLEVVTLGENSITEDDLL
                     VHDQYQQDPGIHLMLAKMAPPHFPMAFGVIRSAMYPTYDDLVEDQISTAKATSKIKCV
                     DDLLNSGETWEIK"
     gene            complement(43803..45650)
                     /locus_tag="PKI35_02870"
     CDS             complement(43803..45650)
                     /locus_tag="PKI35_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNX43353.1"
                     /translation="MSHHSNVIEKEDIVIKFVGDSGDGMQLSGTLFSDTAALQGLDLA
                     TFPDYPAEIRAPQNTTAGVSGFQVHVGTKKIDTTGDQVDILVAMNPASLRSNLKWAHP
                     GATLILDADAFDEASVKKAGYASNPLDDESLSGYNVVKAPVSSLTRAALKPLNLDVKS
                     ADRTRNMFALGICYFLFGWKLDSTIDYFKVKFKKKPEMIEANKIVLEAGYIYADNIEA
                     LASKYHISKAKMKPGKYRNITGNIATAWGLLAASERSGRPIFLGSYPITPATDILIEL
                     AKHKSLGARVFQAEDEIAGICSAIGASYTGSMAITTTSGPGLSLKSEAIGLAVMTELP
                     LVIVDVQRGGPSTGLPTKSEQSDLMQALFGRNGEAPVVVLASSTPADCYFYAYMAAKI
                     AMEHMTPVILLSDGSLGNGSQLFRITKVSDLPPITPPIAEANDATYKPYLRNPETLVR
                     KWALPGTAGLRHRVGGLEKENITGNVSTDPLNHELMVKLREAKVQKVADVLPEQEIIG
                     EKEGDLLVISWGGTRGTTQDAVEAMQAAGKKVSLAHFNYIMPLPKNTRQVLSGFRKIV
                     VCELNAGQFVNYLRMKEPDFSYIQYNKLQSLPFTVAELTAKFNEILEEK"
     gene            45959..46126
                     /locus_tag="PKI35_02875"
     CDS             45959..46126
                     /locus_tag="PKI35_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX43354.1"
                     /translation="MAYQISDDCTACGTCIDECPVEAISEGDIYKIDPDMCTECGSCA
                     DVCPVEAIHPA"
     gene            46229..46951
                     /locus_tag="PKI35_02880"
     CDS             46229..46951
                     /locus_tag="PKI35_02880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HNX43355.1"
                     /translation="MNSDPSDKNNVPLLISFILVNPAVPANVGAAARAMKTMGFSDLR
                     LVNPCNHLCPEARMLAHASNEILEKAGVFPSLHEAAADLDFIIGATARRRTKRGLYVR
                     SNLLAQVIAEKGNTIKHTGIVFGSEESGLSNDDASVCHLLTSVSMAGKYPSLNLAQAV
                     MVYAYELSALKGRKLQAKKAGFDTDGLQALKTKTETLLDEIGIAVDNSNRIHLIERLG
                     LLGEKDVHLLHAIVSQINKKLK"
     gene            complement(47005..47760)
                     /locus_tag="PKI35_02885"
     CDS             complement(47005..47760)
                     /locus_tag="PKI35_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HNX43356.1"
                     /translation="MMDTSELLTSWVDQYSVQWNDCLATGFASLEAMCNYFQESAWHH
                     ADHLGFGFQKLKNTDRAWVLARITMKIDRYPAWRESVIVKTWPTGVEKIFARRDFLIL
                     DTNGQTLGSGASWWIIINPQTRQPQIPEVVRDLILEPPLDEYKPERLRLPETLEPLGN
                     HTVQYLETDRLGHVNNTRYAAWILNALGPDWNNRYKIESYQIEFVTEAFTGERIGLFS
                     SPAGESRFYINGKRERDGKEIFRARIGVKEKES"
     gene            47910..49514
                     /gene="nadB"
                     /locus_tag="PKI35_02890"
     CDS             47910..49514
                     /gene="nadB"
                     /locus_tag="PKI35_02890"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNX43357.1"
                     /translation="MRKYVDFLVIGSGIAGLSYALKAAGKGTVCIITKNSAEETATKY
                     AQGGIAAVTYTPDTYEKHIQDTLIAGDELNDEKIVRLTITEAPERIRELVEWGVEFDK
                     TASGKYDLAREGGHSEFRVLHYKDQTGLEIERTLLRQARENPNIEILENHFTVDLLTQ
                     HHLGMEVNRSTPDLTCYGAYVLNKNNNRIDTILAKTTLMATGGAGNVYANTTNPRTAT
                     GDGIAMVHRAKGVVEKMEFIQFHPTALYYPAEKPAFLISEALRGFGAVLKDRNGNEFM
                     QKYDKRMSLAPRDIVARAIDNELKISGEDHIYLDCRHINKEDLLSHFPGIYAKCLSVG
                     IDISKEMIPVVPSAHYICGGIKVDEFGRSSIRNLYAAGECSSTGLHGANRLASNSLLE
                     ALVFSHRAVTDASSKIKTIAFKDEIPDWDAEGTVMTEEMVLITQTIKELQAIMSNYVG
                     IVRSNLRLQRAADRLAILRRETDALYKQSVIIEKLCEVRNLITVAGLIIRMAQERKES
                     RGLHFSIDYPKSNEKEEMFFNHGDEL"
     gene            49578..50147
                     /locus_tag="PKI35_02895"
     CDS             49578..50147
                     /locus_tag="PKI35_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNX43358.1"
                     /translation="MPLIYPIKGISPRLGANVFIAENATIVGDVEMGNDCSVWFNAVV
                     RGDVHFIRIGNNVNIQDGVVIHCTYRKYPVTIGNNVSIAHRAIVHGCTIHDNVLIGMG
                     AIIMDGVVIESNSIVAAGAVVSQGTVVEQGSVYAGIPAKKIKSVDTSLFEGQVKRISD
                     NYTLYASWHDPSIVPAGYKGQDESNEDRV"
     gene            50533..51207
                     /locus_tag="PKI35_02900"
     CDS             50533..51207
                     /locus_tag="PKI35_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNX43359.1"
                     /translation="MYILIFIVFMILSLIISEVLKSKFRKYSKIPVSRGLTGADVAEK
                     MLREQGVFDVKVVSVEGQLTDHYNPQTKTVNLSRDVYYGNSISAAAVAAHECGHAVQH
                     ATRYGWLQMRSTLVPVVSFASNWVQWVLLAGILLINSFPQLLLAGIALFALTTLFSFI
                     TLPVEINASQRAIAWLTNSGLTTYETRDKAEDALRWAAYTYVVAALSSLATLVYYIMI
                     FTGRRD"
     gene            51380..52621
                     /locus_tag="PKI35_02905"
     CDS             51380..52621
                     /locus_tag="PKI35_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX43360.1"
                     /translation="MKIHLTVISVLCFLISAQTRAQICGCTDSLAINYNPAATLNDGS
                     CIYNSASIAPLSSVTLPGVVSETSGLAAWNGCLWTHNDNSDTRLYALDTASGAIIDTV
                     QLNNVVNTDWEEISQDGDYLYIGDFGNNLNGNRTDLKILRISKSSILTNDPIIDTINF
                     AYSDQLDFTPAGANNTDFDCEAMVVSADSIYLFTKQWISKGTMVYSLPKIPGTYSAYP
                     VSSFDIGGLITGATYLENKKVAMLCGYSTLLQPFTWLLYDFSGHRFFSGNKRKISVSL
                     PFHQTEAITTCDGLNVYMTNEAFSQPPVINNPQQLHIFDFSAYLSQYLNNLIMNLEIP
                     AGEEEVFLYPNPAAGFVCLRFDDSLSDKKFSLLNSTGSAVLFGTLKGNSAIIDLTSIR
                     SGIYLLKIEESNRRAVKLIRR"
BASE COUNT        14378 a        12674 c        12263 g        13465 t
ORIGIN      
        1 agccctcttt tttttatttt cgcttcatgc aaactttccg atacctctca gccgccatta
       61 taatactgtt atcttttacc agccaggcaa cgacagggta ttcctcccgg tgccagaaag
      121 cgcatgcaaa tatactatgc ctccgttttg aggaagctca aactctgatc cgggaggaaa
      181 taaagcagaa tcctgaaaac acacagccat accttcttga aaattatatc gatttcctta
      241 ccgtgatgat cggagaacag gaagaagatt atgcccggct gaaacaacag cgcaacaggc
      301 ggttggcaaa actggcttca ggcgatgcct cgtcaccctg gtaccgttac agccaggcaa
      361 tggtatatat gcaatgcggt tttgcccggg taaaattccg cgacttcgta actgccggcg
      421 tagagctgaa ccaggcttat aaattgctgg aagaaaacaa ccgccgtttc ccggaattcc
      481 tgcctgataa agtatgcctc ggattgctgc attcccttat tggcactgtc cccgacaact
      541 atcgctgggc agtgcggact ttaaaattca ggggaactat caggcagggt ctcagcgagt
      601 tgcagacagc cttccaccaa tcggccagga atccggaatt cagttttctt taccccgaaa
      661 gcgctttcct gcttgcgttt acttcgatca acctggccgg aaatcatgac gcaaccgccg
      721 gctttattgc cgaagccggt aagcagcctt tgcagcaatg gattcacgac agccccctca
      781 tgtgttatgc gctcgctaat gtttacctga aaacaggcga gaacgataaa gcaatctccc
      841 tgttgaaagg acgcgtacag ggaaaaggaa tttatccttt ttactaccag gattatatcc
      901 tcggagtggc aaagctcaac cgcctggata aggatgccaa tattccgctg cttaccttcg
      961 tggcaaactt caaggggctg aactatataa aatcggcata ccagcatata gcctggtatt
     1021 acttcatcaa cggtgatatg acaaaatacc ggctttatgt aaaccgcatt cccctcaggg
     1081 gaaatgtaca ggttgacaat gaccgcgaag ccatgcgttt tgctgaaaaa aaagcagaac
     1141 aaaatatcca cctcctgaaa gccaggcttc tgtttgacgg cggatactac gaaagggcaa
     1201 tgtctgaaat tcagaatttt gaggcaagag ccggaaatac cccacagcgt gaaatcgtcg
     1261 agtgcacata ccgtaaaggc cgtatttatg acgaatgggg caaagacgac ctggccattc
     1321 cggcctatca atccgtcatt aacctgggaa acaaccagcc ctggtattac gccgccaacg
     1381 cttcattgca actcggcatg atctatgaaa accggcatga tgaaaaaatg gcgcggtatt
     1441 atttcaataa atgccttagc ctcaatttcg aggagtatca tttcagtata acccacaagg
     1501 caaaagccgg gttgaaccgt ttagacaata aataaaaaac ctcagacggc aatccggcca
     1561 tctgaggcga caaacaagct ttcccggaat ctaataccgg aatcccaaag tcacggaaaa
     1621 gtaattctgt tgcacggcag tgcttgcggc aggaaccagt gaaatatcat acaggtaata
     1681 atcgtcgctc atccaggcat gggtataggc gaaatcgaca aaatatccct gatcatgaat
     1741 gccgatgccg catgacaagg tttttttcga gccgtcattt ttattgaggt ctttcacata
     1801 aggtgaaccg taataaccga atccgcccct cagggtcaca atttcatatc tccattcacc
     1861 gcctatcctg aaattatgag caggcgccag tttggtccgg atagcttcat tcacatagta
     1921 aaaatcgtca ttatcagcac cggaggtatt acgcaggcgc gccgttgaat aatccaggta
     1981 ttcataatca gcgctgatca gtccgctttg tccgatgata aaggcaacat tacccatcag
     2041 ccgcatcggc gtgtttattt tatagttata acttccgttt ggcgaggttg ctgaaagccc
     2101 agtagcctga tcgaattccg atgtcatctc ggaataatat tcatcggtca tgtcattata
     2161 ataggtaggg gtatgtacag caaagccaat gcgtaagaaa tcaaggggcc ggtagatgat
     2221 gccagcttta aaattcacgc ccgttccgcg ggtttcaaaa tggtcgtgtt ttgtaaatga
     2281 cttgaaataa tcaatattgc cttttgtatc ggtctcggtc agcctgctat cctcttcata
     2341 acggatataa ggaaatccga tggtagcccc gaggtaaagc ttatcgctga aattcgcccc
     2401 ggtactgagg accatttcat taaccgttcc ccagctctcc accgttttcc gctggtcgat
     2461 tccgccctcc ggggcatcaa tccagtaatc ctggctggca gaatcatata ccagaacatc
     2521 tgtattatag gccagttggc ttccgaatgg gttgaggtga tcgtaatcaa gcgggttgcc
     2581 gtcattattt gcttcgtaca tatattgcgt cagcagggaa ctttgattat tgaaaccact
     2641 gatgatcatc ctgttgttga aattagccag acggttcata ccaaagccga agttgagata
     2701 cttcagaatg cttgattccc ggttgcctcc gatgttccag ttcatcacca ggcccacgtt
     2761 accgaggtta aagctgtact tgttgtcgct gtttttctcc ccgaagaaat cagcttccgt
     2821 tgaggttgta tttaattgcg gggtgatggt gaattccgat ttacggtaaa ggccaagtcc
     2881 tgccgggttt tggctgagtg tggagaaatc cgcgcccact gccccaaagg ctcctcccaa
     2941 tcccagaaaa cgtgccgtcc cgccatagga taacctggaa tatctcaggg cgtcattctc
     3001 attttgcgca attccgctta aagcagcagc ggcaaaaatt accagtatta atatcttttt
     3061 catgctcatt gaaattatta tcttgttcaa tcggtaaact gcatactcct gtatgatggc
     3121 tgaatcttac cgacatgcat cacattgatt tctttaagaa tcagcactta cgtccggatt
     3181 atctcctgtg tccggagctt gaagaacccg acgatgagga actgctgctc cgggaagatc
     3241 ctgaagaact gctgctgccg gaatgcgacg gggatgaata gcttccgctg ctgcgcgaag
     3301 gtgcagaata ggtactgctg ctgcgcgaag gtgcggagta tgtgccgctg ctgcgcgaag
     3361 gggtcgaaga tgaattattg cgtgaaggcg ctgaataatt atcatttctg ggagccggag
     3421 cctggtaaac tttattctgg cgaggcgccg cctgctggtt gtatgccggc cttgaaggct
     3481 gagaataaga gcggttttga ggagcaccag agccctggtt attacgctgt gaaggagtta
     3541 cgtcagtttc gcgggggtta cggtacttgg gcgttgtgta ttcctgtgat gatctgggtt
     3601 ttgcataggc aggagggctg tatggctgca cattggtgcc gcgttgtacg ccttgcgtac
     3661 cgggagaagc agcattgcgt gacgacgggt ttgcatagcg ctgctgacgt tgctgaatga
     3721 tttcaggttg ttgtgaacta ttccggtcgc cctcagcagc attagccttc ggtacttcgc
     3781 ggctggcatt ggcagcgccc gggtttacat accggtactg cggggtgcgg gctgccggtt
     3841 cattcgaacc ggttgcggtg cgttgcatct ctgcattacg gggctgacga tcgccggttg
     3901 ccggggttcc gcttccaatg cccgaattcc gggagttatt atcgataacg gttgcgttgc
     3961 gcgaaccggc tgcgggctga gcagtaatat cacggcttgc tccgctgttg ttgcgcgatg
     4021 ggctgatagt gccggaacct ccaccgtcac gccgggtttc aacggcactc cgtgtacggc
     4081 tcgaagcaag gcctgcttca taacgctcgc cgaaggtacg gtaatggtta ttccggccaa
     4141 gagatcccga agatcctccg ccattgcgag ggccataata tgaggagtta tggtcgaagc
     4201 tgttgtaata attatcatga tagccaaacc agtagccatc ataaaaaccg ttccagtatc
     4261 cgaggttata tcctgaataa aatccgcttc cgccatacca cgggtacgga tcataccagc
     4321 cataatttgg atagtaccag tcgtaccagc cgtgacgata gtatgaagga tagtaatagt
     4381 aggaaggata ccagaagtta tacccaagat aaatactggt tccccagtta aagggatcat
     4441 agtcgtacca gtacatattg gtataatagt cggagtagta actgttaaag caatagggat
     4501 tatgaaaacg cctcagcctg gcggcatagg cataatcata ataatcgtcg gaatagaaat
     4561 tattgttaat ataggtatcc ccttcgccgg cagaataatt ggcggtatcg ctgtttgccg
     4621 gctcagcata cccctgagct gtttcagcat tcgcggcctg aggttcggct ggctgaacct
     4681 gttgcggctc aactaccgta cggatttcca acggcttatt tttcgatgaa taatacacat
     4741 catcgtagtt tcttgcctgg tattgcgtag tacatcccgt aataaatatc aggcttacag
     4801 cgaccaaagc aatgtagttt ttcatgattg acatctcctt gaattattta ggcttcttta
     4861 aggcggctag agttgatatg aggcattcgc catatttttt gtacttttgc cggtattccg
     4921 acatcaaaaa tattgcaaaa attgtaccat atccaatcat tatggcaaaa gatttaacaa
     4981 cgcgtgaaga gaattactca caatggtata acgatctcgt aatcaaggcc gatcttgccg
     5041 ataactcggc ggtaaggggt tgcatggtga taaaacccaa tggttatgct atctgggaaa
     5101 aaatgcaggc ccaactcgat aaaatgttca aggaatccgg tcatgtcaat gcatactttc
     5161 cgctctttat acccaaatcc tttttcagcc gcgaagccag ccatgttgaa ggattcgcca
     5221 aagagtgtgc cgttgttacc cattaccggt taaaaaattc gcccgacggc aaaggcgtcg
     5281 tcgtcgaccc ggaagcaaaa ctagaggaag agctgatcgt tcgccccacc tccgaaacca
     5341 tcatttggga cacctaccgg agctggattc aatcatatcg cgatttaccc atactcgtaa
     5401 accagtgggc caatgtagta agatgggaaa tgcgcacccg cctgttcctg cgtacggctg
     5461 aatttctgtg gcaggaaggc cacacagccc atgcaacccg ggaagaagcc attgcggaaa
     5521 ctgaaaaaat gctgaatatc tacgccgaat ttgccgaaaa atggatggct attcctgtcc
     5581 tcaaaggttt taaatcgcca aatgaacggt ttgccggcgc agtggaaaca tattgcatcg
     5641 aagcgctgat gcaggatggg aaagcccttc aggctggaac atcccacttc ctgggacaga
     5701 attttgccaa agcatttgat gttcagtatc tgaacaagga taaccagctc gattatgtat
     5761 gggctacttc ctggggcgtt tcaacacgcc tgatgggagc gctgatcatg acccattccg
     5821 atgatcatgg cctagtaatt ccaccaaggc ttgcccccac gcaggtggtg atagtcccta
     5881 tctataaaac cgacgatcaa ctggcggtta tatcggagaa agcgcaggaa atcatgaaag
     5941 ccctccggtc gaaagggatc agcgtaaaat acgatgaccg agacacattc aaaccgggat
     6001 ggaaattcaa cgaacatgaa ttcaaaggcg tgccggtgcg cattgccatc ggcccccgcg
     6061 atcttgaaaa cggcacggtt gaggttgccc gccgcgacac cctcgaaaaa tcagtatacc
     6121 agatcaccga tcttgagact aaaatcgaac acctcctcga aaacatccag gataatctct
     6181 acagaaaagc gctggttttc agggaagata acacttacca ggccgacacc tgggaagagt
     6241 tcatcgacat actcgacaac aagggcggat tcatttcggc ccattgggac ggtacgcccg
     6301 aaaccgagca gaaaatcaag gacgaaacaa aagccaccat ccgccttatt cctttaaata
     6361 ataaacagga agcaggaaaa tgcatctata ccggcaaacc atcaacacaa cgggtgattt
     6421 ttgcaagggc ctactaatct gatcattcat actccggcgg tttaatagct gccggcaggc
     6481 gaatattatc tcaaactcct aattttcagt cacgtgaagg ttcttggcgt aattccggca
     6541 cgatatgcat ccacccgttt tccggggaaa ccgttgacca tgattgccgg aaaaagcatg
     6601 atcaacaggg tatatgaaca atgtacccaa tgccggttgc tgaatgaagt cgctgtggcc
     6661 accgacgacc agcgcatagc agatcatgtg aaagagtttg ggggcaacgt tttcctcaca
     6721 tccgacaaac accgctcggg aactgaacgt atcggagaag tattgcagat actgaaagac
     6781 gaaaatccct ccgatcattt cgacctggtt gtgaatattc agggtgatga gcccctgatt
     6841 tcgccggacc agatcgtaaa aacaatcggt tgttttaagg attccaatgc cgaaattgcc
     6901 acgcttatca aaccgatcag tgacagcaag gagcttttca atcccaatgt cgttaaagtg
     6961 gtcatcaata aaaacggtaa cgcactctac ttcagcaggc aacctattcc atttctccgg
     7021 ggagttgaac ccgcagaatg gcttcagcat catggatatt ttaagcatat cggcatttat
     7081 gcatttaaaa cacagatact tgaactgatt gtccgcctta caggttcccc gcttgagaaa
     7141 gccgaatcgc tggaacagct tcggtggata gaaaatggtt tttcaataaa taccgcaatt
     7201 actgattttg aatcggttgc cattgatacg cccgaagatt tatcaaaact tactaacatc
     7261 ttatagcatt tcccggattc actccgtaaa ttagccggtt cagaataatt caaatgacca
     7321 tgatagggcc agccatcagc gaattattaa ggggacatga atgcgtaatt gtccccgggc
     7381 tgggaggatt tcttacctcc catgcatcgg catgcattga tgaaactacc ggtcagttca
     7441 tgcctccccg aaaatcggtt gcttttaatg ccagcctctc caacaacgac gggatactgg
     7501 cgtaccacct tgcctgtaag aacaatattt cctacactga agccctgacg agggttaaag
     7561 actggtcata cagcatttta cagtctctca gaagaggcaa tgccgttgag atcggccatg
     7621 tcggcaagct cagtcttaac actgccggca acatacactt tgaaccggat gcagaggcca
     7681 attatcttga tgaatcatac ggactgccct ttttcacaaa accggcattg aatatgcctg
     7741 ttcaggtaat tcaagagtca gcgtcagccc gcaaagtaaa agaaagcatc cgcaacattg
     7801 ttccctctac cctgaaatgg gctgctgtgc tggcgccgct catcggtttc ttcatctggg
     7861 gatcggtaaa caccccttct atcgggaatt ttgtgaacaa ccagtccggt attttcgcct
     7921 ggtcgaatac aaccccgggc aagacgtcgg tcatcaccgg gaaccaggct gccaggccgc
     7981 ctgttaccgc cgatatcact tcaccggctg agatactcgg ccctgaagcg tgcctgatat
     8041 ccccggcatc aattccttac gaagcaatgc attcccgcgg ccttacgatc acggaacctt
     8101 gcactgatgc ggacacgaag agcaatgccg aaaagccttc ttattttatt gttggcggag
     8161 cattccgaga agttaaaaat gcaggccggc tcgtaaaagc gttgcaacaa caggggtatc
     8221 ccgccactat cgccgatacg acgcataagg gattatacgt ggtaagcatc cggtcgtttt
     8281 cggatagaga agaagctttc aggcagctaa aaactataag ggagagcggt catccttcgg
     8341 catgggtatt gaaagcaaaa aattagagat tgcctgcctg tcgcaggccg ctgacatgca
     8401 ggcccaaatc agtgtttcca ttgccagggg cagagttatc ataagccctg tatgtcattc
     8461 ccgataatca acttagtatt caatcctgat ttcattgtat tttggctaag cgaaaactgg
     8521 aacgttttgc agaaaatgcg accttcccgc atatgtttca attaatgtgg gacgacctgc
     8581 aggccgggtt cgggctgaga gggagatggc gcaacgatta tttccggaac gaaaatcccc
     8641 tgatcctgga gttggggtgc ggcaaaggcg aatataccgt aggcctggca acgcactacc
     8701 ctgataagaa ttatatcggc attgataaaa agggagcccg gatgtggcgc ggcgctaaga
     8761 ccaccgccga aaaacaactt cccaatgtcg cttttgtcag aatgcagata gagggaattg
     8821 ccgatgtatt tgcccccgga gaagtcgatg aaatatggat tactttcccc gaacctcatc
     8881 ccaacagtcc ccggacgaaa aaacggttta cctcccccca gtttattgaa aggtaccgtc
     8941 agatcttaaa accggaagga atcatacacc tgaaaaccga cagcgacctg gtttttgaat
     9001 atacccatga catcatcaaa gccggcaaac acaggctgct atactccacc agcgatctgt
     9061 acagcaactc atcggatgaa atggtaaggg atgtagttgc cgttcaaacg ttctatgaac
     9121 aaatgtggct ttcacaggga attccgataa aatacctttg tttcacaatc aattaaatgc
     9181 ctgccgtttg aatgatgcag gatgaatcgt ttttcgaaaa ggtttatacc gtcgtcatgc
     9241 aaatacctgc cggcagggtt acttcttacg gcgctattgc aagatatctg ggcaccggac
     9301 gatcggcccg gatggttggc tgggccatga atgcctcgcc tacctgcccg atgccggttc
     9361 cggctcacag ggtggttaac cggaacggcc tgcttaccgg caaacatttt tttggcggga
     9421 acaagatcat gcagcagctt cttgaaaatg aaggagctat cattcaggac gatttgattt
     9481 taaattttaa agaattgttt tgggaccctg caaaggaact ggaatagcaa cctgattatc
     9541 cggatcatca cttttcactg aaaaagggat ccagattata gccctgcccg agtatccggc
     9601 cccggttatc catatagaac tgacgtactt tattgaccgg tatcacaaag gttatcccgt
     9661 agtttatttc cttttcttca tgaacaaata tgttgccgtt gtaaggaatg gcaggattgt
     9721 aagcatattc atgtatctgt ttctccggct tgagcgaata accggtcctg gccgaaaccg
     9781 ccctcccgat cccgaccagt tcaaagttag gaacgccatc gcgcacagcc atgacgatgc
     9841 ctccactaaa gccttcgttg aatgaagcat cggtcatgaa attcccgttt ttttcggcag
     9901 gcttgctgac gatgcctttg gttaccatga gttggcccaa cggatagcct accaggtaaa
     9961 taaagctgcc ccattccagt tggtcagaat agccgaccgg atagctgaat acttttaccg
    10021 gcaaagcagg ctgatttaca gttcttccca ggattgcaat gtcgtttttc gaatcactcg
    10081 ccagcacctg tatccggccg ccttccggaa tatccctgat atacagttgc tgattggtct
    10141 ttagcgatat actgtggata aatctgttcg actgcaggtc gctgtgttcc gaccagtgaa
    10201 tgattgtatc gggaaaaacg gcgacatggg cacaggtgag caaagccagt ttatcaccgc
    10261 tgtaagagat taccaacgcc gtcccgctga ccgattcatt gttttctatc ctggctttgg
    10321 ctttttcgaa taaatcagat cctgaaagat cccgtaaggt tatctctgaa ttttcgccga
    10381 aaacataagt ccggtaggtt gcataactgt taactttttt caccgaacgg gtaatttctt
    10441 ccagttcgtt ggcacaattg cgatagggaa actcactgtc gtaacggcca tcctttaatg
    10501 ccggatttgc cgtttgcatt acctgccggg cgcaccccgg ggataaaagc gccaggatta
    10561 aaaggattcc ggccggttta aactgtttca taatctctga tatcaggatg tcaggcttca
    10621 ttcaaaaaga agatacaatt tacaacttaa gcctcaatta tctgtcacga caaaattgaa
    10681 tttcatatca ggaattttat ctatctttac gctgtctaaa taaaaataat ctgataaagc
    10741 atggaagtat cgaaagattt gattttaaat gccttacgga atgttcagga tcctgactta
    10801 cgaaaggaca tcgtcacatt gggcatggtt gaagacattg tcatagaggg aaagcggata
    10861 aatttcaaac tcgtgctcac cactcctgcc tgcccgatga aggggaaaat gaagaatgac
    10921 tgtatcgagg ccatccactc ggtcgttgat cctgatgcgg aggtgaatgt tgagttgata
    10981 tcgcgcacaa cctcgcgccg caaagccggt gaaaatatgc tcaaagacgt taagaatatc
    11041 attgcggttg tatcaggcaa aggaggagtg ggaaaatcaa ccgtggcagc caaccttgcc
    11101 gtctcgctgg cccgcctggg ggctaaggtc gggttacttg atgccgacat atacgggccg
    11161 tcggtccccc tgatgtttga cctcatggat gcccgccctg aagtaattga agaaaacggg
    11221 caggctatca tcaggcccat cgaaaaatac gggataaaac ttctttccat cggttttttt
    11281 gtcgatcctg acaaagccct ggtctggcgc ggtcccttgg cttctaatta cctcagccaa
    11341 ttgctcaccg gcagcagttg gggcgaactc gactacctgg ttattgatac gcctccggga
    11401 accggcgata tacatcttac cctggttcag aacgttcctg tgaccggcat tgccatcgtc
    11461 accacgcctc aggaagttgc cctggctgac gcccggaaag catacgggat gttcgctgcc
    11521 gaaggcatca aggtcccggt gctcggaatc atcgaaaaca tggcctggtt tacccccgaa
    11581 gagttgcccg gtaaccgtta ttacattttc ggacgggaag gcggaaagcg ttatgccgat
    11641 gaaatgggca tcccgttgct cggacaaata ccgctggtgc aaggcatctg tgaatcgggc
    11701 gataatggaa gccccatcgc aataaacgat tcatcccccg ttgccgttgc attccgcgac
    11761 ctggccgaaa ccatagcccg gcaggtcgcc atccgcaatg caaccctgga agccaccaaa
    11821 gtggttgaaa tgaaatcgta acacaatcct taacaaagaa tccaaacctt tttgatcaac
    11881 aataactccc taacaatgaa cgcagaatta gaaagcaaag taaaaaatgt catagaccag
    11941 atcaggcctt acctgcaggc tgacggcggc gatatcaggt tcctggaact tactgatgaa
    12001 aacgtggtaa atgtcgaact tcagggttcc tgcggatcgt gcccattcag tcgaatgacc
    12061 ctcaaaaacg gcgttgaaga agccgtaaaa cgcgcacttc ccgaagtaaa atcggttgag
    12121 gccgtgaaca tttagtcact gtttctatgc cggaataaaa gcagtttctc tttcggaagc
    12181 tgcttttttt tatcattact taccggctgg acgagtttat tcattgaagc acaaaaatca
    12241 cctcacccca aacccctctc ctcaaggaga ggggcttctt tcaacaaaat ctattgtttt
    12301 atcaggcagt aaatcacgaa aggtatttcc ccacgatagg catacgcctg ccgctgccga
    12361 acgctttggg agaaacacgg aggataggcg cggtttgata ccttttccat tcattggtgt
    12421 tcaccatttt aagcacccgt cttactatag cttcatcgaa tcccatggcg atgatctcca
    12481 acggcccgat ccgcttttcg atatactgat aaaggatttt atcgaggatg gaataatccg
    12541 gcagcgagtc gctgtccttc tgcccgggac gaagctcggc cgaaggcggc ttggtgatac
    12601 tgttcagcgg gatgatttct ttttcggcat tgatataacc tgccagatca taaacttcgg
    12661 ttttataaac atcgccgagc accgataatc ctccgcacat atcgccgtac agggttccgt
    12721 atcccacggc cgcttcactt ttattggaag tattcagcaa tatatacccg aatttattag
    12781 ccattgccat gagaatgaca gcccttaagc gggcctgaat attttcttcg gttacattaa
    12841 acggcaaccc tttaaaataa ggctgcaggg tttccagcac gctgtcatac cctttgcgga
    12901 tgccgatcag ttcgtaacgg acgcccaggt tttcagccaa ttgccgcgca tcgtcaaccg
    12961 aatgctgcga tgagaattcc gaaggaagca ataccgccat gacattttca ggtcccagcg
    13021 cccgggctgc aagcgccagc gttacagccg aatcgatacc gcccgaaagc cctaatatgg
    13081 cctttttgaa acccatcttc cggaaataat cgccgatccc catcaccagc gcgtcgtgga
    13141 tgagtcctgt tttcgatgaa gtttgatgag agggatgaac gactggttcc ggcgtcagtt
    13201 taccttcaac aaatttaaaa tccacgaatt caaggccctc ttcgaaagcc ggaagcatcg
    13261 ccgccacatc cccgctggca tcaagcaccc gggacaaacc gtcgaacagc aactccgtct
    13321 gcgctcccac atggtttaca tacaccagcg gcagcccgta acgaccggca tttttggcca
    13381 gcacctcccc cctttcgttt tcctggtcgt agttgaaggg agaagcggca atattaatga
    13441 tcagatccgg ttttaatccg atcatctcat ccatcggggt agccacatat aacgggtcgg
    13501 ggccgatgtt ccagatatct tcacaaatgg tcagggcgat ctttatcccg ttatattcca
    13561 gcacattaaa ttgcctgttg ggttcaaaat aacggtactc atcaaagata tcgtaattgg
    13621 gaagcaacgt tttaaaagcc gttcctttca cttccccgtc ggcgagcagc caggcggcat
    13681 taaagaggtc cttacctttt aaatcaggat ttcgggcggg cgccccgatg atggcagcaa
    13741 tcccgatgca ttcagcggca attttttcga tggccagtcc gcaccggttt ataaaatcat
    13801 caaattcgag aaaatcacgc ggcgggtacc cgcaaagtga caattcggcg aaaactacaa
    13861 ggtcggcctt ttgtctgcgg ctttcccgga tggcatttgt gatttttgcc aggttacctt
    13921 caaaatcgcc gattacataa ttgagttgag ccagggctat acgcatgatc tcaggtataa
    13981 attcgctgta aaagtaaaaa gcccggcgct atttccgggc ttttccaaac gatttctttc
    14041 aacaattaaa agaggacaaa ggcattcagc tcaagaaaat tgatgtatcc tttttctttc
    14101 aggctgccgt caaccttatt cttaccggtc agcatatcgg taaaaccgtt gttgaaggtt
    14161 aatcctgccc cgaaagtagt cccggcatcc agggtatact cagcgcctaa tccgataatc
    14221 agcgattcac gaagaaaggc gatgttgctg atattccctt ccttggtcac aaccgtgctg
    14281 ttgtaagtaa attcatcctc gcttttggca tccagttgaa aactggtccc taaaccaatc
    14341 tgcccgaaat actgaaaatc gccgatgggg ttggtccgca tttttaaggt aagcggcagt
    14401 tcaatcgcct taagcttata ggtacgtttc atggttccgg catttgttcc cgatatatcg
    14461 ggatactgga gtttccctcc gttaaacagg acgttgaatc cggtggcgaa ggagtggttc
    14521 tcggtgaaat tcatttcggt gacaaacccc cagccgaagc cgagtttcgc cccgtcgttt
    14581 gaatagccct ctacattggt acgcatccag cttatttgcg gagcagcttt caacccgaat
    14641 ttgaattttt tgtattgcgc gttggccgtg aatcctatca caaggattaa tgacagcagg
    14701 aggagctttt tattcataac tttgatctta atgataaaac ttcccgcaaa tatcgctcaa
    14761 aaacatgaaa aagataacat cactgaaaac atataccaca aaaattcagc ctggtttttg
    14821 aagatcgacc ggacgctttt gtaaaaagta taccataact tactattttt acgttatcaa
    14881 ccgatcaaac tcaccgtttt atgaaacgtg ttctcttcgt cgcctttctc ctgagtgttg
    14941 ccgtttcctt cgcaacagcc caaattccgg gattttcgat agggccgaaa gttggttata
    15001 attcaaaccg ccttgttacc aatctcgatt cggtgacttc tacgcccgaa ggggctttcc
    15061 agatcggcgc tttcatgcgt ttcggacaaa aagtatactt ccagccggaa gtgaattata
    15121 ttgtgaaagg cggaattatc aacgacgttc tcgatggtga agaccgcagc caggaaattg
    15181 agcttaagag tatcactatc ccgctcctgc tcggagtcag gccggtttcc gaaggatcct
    15241 tcaacatccg tctgatggca gggcctaccc tttcctatat ttccgaaaag catgttaaac
    15301 cgctcgaact gctaagctca tggccgatac actcaaccga tgacatcgat aaaatgctct
    15361 ggtcatttca ggttggcggc ggcatcgatt tctggattct cacctttgat gcccgttacg
    15421 aggtaggcat cagcaatatt tacaccggcg acgaaaacct caaagtccgc aacaacctgt
    15481 ttaatgtgag tgtaggcgtg aaattcctct gacagatcgc ggcaacatta acccttaaaa
    15541 attttcttta aaaccggaat ccatgatcat gggttcccaa acagacatct cttttaaatc
    15601 catgcgcaga tttttctttt tattgttgtt tgcttccgtt attctcttcg gatcgtgcca
    15661 tcgcaacaag caagtcttca tccccggccc ggtcgctaaa gtcccatcgc agccggttca
    15721 gataatgcga tacgacagcg cccttttttc gatcaacagc cagaatctga aggaaagcct
    15781 ggttcccctt gccgggcgtt attatttctt tctgggctcc gactggagtg acacgatcaa
    15841 tctgatgaac ctgtcgcaat tcgtcaacga ccccaacatc aggaagttgt atgagatcgc
    15901 gatgcaaaaa tacgggaacc tttccgccct taaaaaagac ctttccggag caattgcccg
    15961 ctaccgctcc tattaccccg aaaaaccatg tcctctcgtt tacacgtata tttcgggact
    16021 taacgtggaa atgccggtgc tgtttaccga ctcggcaatg gccattggcc tcgatctgtt
    16081 tctcggcaac gatcaggtca tttaccggga agcaggaatc cccgagtaca aaattgcccg
    16141 gatgatgccg gaatacatcc ttcccgaatg catgaaagca atagccggca atgtgcttcg
    16201 aaccgacccg ggcgaccaga cgttactcaa tcaaatgatc acggcaggca aaatctgtta
    16261 ttttctcgat gcgatgttcc ccgaaatggc cgataacctt aaaatcggtt atactcccgg
    16321 acaactggag tggtgtaaaa ccaatgaaga aaacatctgg aaattcctga taggaaatca
    16381 gttgttattt aattccgacc cggcggcggc atccaagctt ctggccgacg gcccgtttac
    16441 ttcaggtttc gcccaggaat cacccggacg gctgggggta tggatgggct ggcagattgt
    16501 ccgcgcatac gccgccaaca acaagaaact gtcgctgaag cagattatgg aagccaccgg
    16561 ctcacaagcc atcctggaag cttcagagta caagccggga agatgatctt ccctcccggg
    16621 caagccctgc aaactaaagg gtttcttttt ttaaaccacg ggctacggag aacgcaaaat
    16681 accacctaga cactcagcac acgcagaggc acttaggtta ttttaccacg gagccatgga
    16741 ggacacgaaa aaacaccgag tgttgcatat tttctcagtg aacctcagcg ccctaagtgt
    16801 cttagtggtt cttttttacc acgggctacg gagaacgcaa aataccactt agacactcag
    16861 aacactcagg gcactaaggt tattttacca cggagccatg gaggacacga aaaaacaccg
    16921 agtgttgcat attttctcag tgaacctcag tgccctaagt gtcttagtgg ttctttttta
    16981 ccacggagcc acggaacacg aagaaacact gagagtcata tattttctcc ttgaacatcc
    17041 atgcccttga tgccccattt tcaggcgaaa cgcaacctaa taattctcac ttcctgacaa
    17101 gcccggatag ccggcaattc atcagccgaa aatttcaggc aactgcatcc gcagcggaag
    17161 tattgacagg atgtaccgct tttgctcatc cgggtaaacg ctccagttgg caatttcttc
    17221 aatattcctg tagcagccgg tacaccgccc gtggtggtca agcgtgcaga cattcctgca
    17281 cggggatttt acctgctgta gtttattttg ctgattccgg tccattttac tgcctcgttt
    17341 accaaataaa accataatcc cttatatttg ttcaggttat cgccgggaaa ggagtgattt
    17401 gtctttaata ttatcaggaa taattttcaa ttcggttttt tatgcttgtt aaaactttcg
    17461 gatgcgctac ccaggggatc aatgccacaa cgattacagt tgaggttaat gtcgatcagg
    17521 ggattcagtt tgcaatggtc ggtttgcccg ataatgccgt aaaagaaagc catcagcgta
    17581 ttgaagcggc gctggtcaat atcggctata agttcccgca caaaaaggtg accatcaaca
    17641 tggcgccggc cgatatccgc aaggaagggt cgtcgtacga cctgaccatt gccatcggca
    17701 ttcttgccgc cagcgagcaa cttctggttg atgacctgga caggtacgtg atcatggggg
    17761 aactgtcgct cgacggcagc ctgcaaccca taacaggagc gttgcctatc gccattgaag
    17821 cgcgggataa ggggttcaaa ggttttatac tgcccctgga gaacgcccgc gaagccgcca
    17881 ttgtcaacga cctggaggtt tacgggcttg agagccttaa cgatgtaatc ggcttcctgg
    17941 aaggaagcct gaaacagacg ccggtagaag taaacacccg cgaagaattt tacaaccact
    18001 taaacgaata tgaattcgac ttcgccgatg taaaaggcca ggagaacatc aagcgttccc
    18061 tcgaaattgc cgcagcaggc gggcacaatg tgatcctgat aggccctccc ggcgccggaa
    18121 aaaccatgct ggccaagcgc atgccttcca tcatgcctcc ccttaacctt cacgaagcgc
    18181 tggaaaccac caaaatccat tcagtggccg gcaaaatggg ccgcaatgcc tcactgatct
    18241 ccgtcaggcc tttccgcagc ccccatcaca ccatatctga tattgccctc attggcggtg
    18301 gcagcaatcc ccaacccggt gaaatatcgc tggcccacaa cggggtcctc tttctcgatg
    18361 aactgcccga gtttaaacga acggtcctgg aagtactgcg tcagcccctc gaagaccgta
    18421 tggtaaccat ttcgagggca aaatccacgg tgacgtaccc ggccagtttt atgctggtgg
    18481 catccatgaa cccgtgcccc tgcggatatt acaaccaccc ggagaaggac tgcgtgtgcg
    18541 gcccgggcgt tgtacagaaa tatctcaata agatttcagg gccgctgctc gaccgcattg
    18601 atattcatgt ggaagttgtg ccggtccctt tccgtgaatt gaacagtgaa agagtctcgg
    18661 aaaagagcga ggccgtccgc gaacgcgtgg tgaaagcccg cagggtgcag gaagagcggt
    18721 tccgggaatc gaaaggcatt tactgcaacg cacagatgtc gagcaaacag ctccggcaga
    18781 tttgccagat caccgaggaa ggacaggtat tgctgaagaa tgcgatggac aaactgagcc
    18841 tttcggccag ggcttacgac cgcatcctga aagtggcccg caccattgcc gaccttgccg
    18901 aaagccccga catccggacc gagcacctgg ccgaagccat ccactaccgc agcctcgacc
    18961 gggagacatg gggcggatga aagaataaca gaggaagaat gacggttaac ggattctgaa
    19021 atgggaaaaa gaagttaatc ccacgcgtcc gtaagtgcaa acatgcataa caacttctgt
    19081 aagaatgtat tatggcaaag attattgtaa aaaatactga aattaccatc atctccgttg
    19141 atgaaaaaga ttacatttcg attaccgaca tggcaaatgc taaagaaagc gaaagccgtg
    19201 cagccgatat tattaaaaac tggttaagaa gccgttatac tcttgaattt ctgggaacct
    19261 gggaacagat caataaccct tattttaaag tggtcgaatt tgaccacttt aaatcacagg
    19321 ccggtttacc caactttgta ttaagtgttt cggagtggat tgaaaaaacc aattctatcg
    19381 gaatcattgt aaaaaaaggc aggtatggcg gcacctttgc ccataaagac atcgcatttg
    19441 aatttggttc ggccattagt gtccctttta aattgtatct gatcaatgaa tttcagcggc
    19501 ttaaagaaga agaacaaaag cagtttggct ggtcggcaaa acgggaactt accaggttaa
    19561 attatcatat ccacaccgat gccatccggc ataatctgat accacaggaa ctttcgccgg
    19621 cccaaatttc cactgtttat gcaaatgaag ccgatgtgct gaatgttgcc ctgtttggta
    19681 ttacagccag gcaatggcgg gatgcgaatc ccggattaaa ggggaatatc cgcgactatg
    19741 ccaccatcaa cgaattgatt tgtttatcca acatggagaa cctgaacgcg gtgtttatca
    19801 acgagcagat gccgcaaaaa gagcgcttga taaaactaaa ccggagcgcc atccagcaaa
    19861 tgagtgtttt gcaggatgcc ggaaaccgta aactgttgaa ataattctac actgatttat
    19921 aaaacagcaa aaacgaagtt agtaaaacgt ctttccccct cagcgtgtcc tccgcccctg
    19981 cattacgggc agccgggaat ctgccgtaaa gaaatattcc gtttattcag gttagacaaa
    20041 atccggaaat ccctattgtt tcactaattg ttcataactt ttttaagaaa tatctataac
    20101 agaaagatat atcgcctatt ttagttggct gatatacagg actcccgcag aacattgaga
    20161 acgcatactg ggacaaggaa cgtaaccaat cggcttgatt aaaagtggga agagtgaggt
    20221 gatatgtgat attaaacggc aagcctaaaa tgatactgca atgaatttat cccattatca
    20281 agaccaagtc aatagacttg aaaaagatgt tgcagacttt caaaagaaga tagcagacga
    20341 aagcagaaaa gagaatgaca aaaaccgtca gattgactca gtaaacaagt caattaataa
    20401 atacacgtca gagtcttcgt tgcaaagcaa actaagacag attcgaggtt atcaaaacga
    20461 tatttataat tgtaaaaaga aaattgcgga atatcagaaa cagatagcta ataaatcagt
    20521 tgaaattggt aggaaaaagc aagatttaag aaatgccgaa gaagccgaac aaaaaaagca
    20581 gcaaaaagat caattagact ttcagcgtaa attacagcaa gatgtagaac agcagaaatc
    20641 tcaattgaat tttctaataa atcaaaatta ctcatcaaag acagagaact ttaatgattt
    20701 agacgataag gagtacgatt tttttatatc gcacgcttct gatgataaag atgagatagt
    20761 aagggattta gttgatgctt tgaagaataa tggctttaaa gtatggtatg acgaatttga
    20821 gttaaagata ggagatagct taaggaaaaa gattgataat ggtttggcaa aatccaaata
    20881 cggaattgta attatctcac cttcgttttt gaaaaagaag tggccagagt atgaattaaa
    20941 tggtatggta gccaaagaaa tgaatggaca caaagtgata ttgcctattt ggcacaaaat
    21001 tacgaaagat gaagtattga agtttagtcc aacattagca gacaagatgg ctttgaacac
    21061 atccattcat agtattggcg atattgtgaa ttcattgaaa gagttataaa aaatgcatgt
    21121 cattaacaaa ttttacaaat caattggcgt ttcggtgcag aaaacaaagt aagtagccta
    21181 taacgatggc cttgcggctc gacagaaaaa ccgcccgcat tcgtcaattt catatagctc
    21241 tggccgttgt atacaatagc aaaatgacat ataaagacga cataaagcaa aaatttgaga
    21301 acattgatgg acatctttgg tggccggata caaacaaatt gagtcaatta aatggaaatg
    21361 ccgacgattt ctttgatcgt actttcccag aaggtaaaat cgcagctctt tttatctatc
    21421 atcagttgat gattgaacaa atgaagatgc taataaaata tattaattat tatatccaag
    21481 tctcactata tccaacagtg attcaagtta ataacttcga gaatgagagc caattcggat
    21541 ctattaatga atattttaaa tatacaattg aattcaaagg taaagcacaa atactaaagc
    21601 aagcgcaaat tttaaataga cttcgtaata actatggaca tagtatgaca gacaaatggt
    21661 tagaagatgc ttgcgataag gaacttagta atctcaaaac tgattattat atctttttta
    21721 aaagttggag taatggaatt aatgagattc ggaaactggt tgaaaaggta aaacaaagta
    21781 aagatataaa gagactaata gaactaactt gaaaagcttc tgttcacata tggtataaaa
    21841 atttaccgta acagattgta attcaagtgc gttaacccgc attatgttca atttatcttg
    21901 ctagaaaatc gctacgcaaa ttgtaacttt caatacaacc aacgttatgc agccgcttaa
    21961 aaaggcttga atgccaaaat aaaccaaaat gaaatggatt ttataacatt agattttgaa
    22021 acagctactt cgcagagaca cagtccttgt gagattggat tgactttcat taaaaatagt
    22081 cagattgttg agacgaaaac ttggttgatt aaaccgatta ataatgaata tgatggattc
    22141 aatattttta ttcacggcat tactccaaaa gatacagcag acaagcctga gttcaatatc
    22201 atatggactg aactaagacc actaattgaa aatcagttcc tcattgcgca caatgccggt
    22261 tttgatataa gcgttttacg cagaactctt gaactttaca aacttccttt cccatcatta
    22321 cactattgtt gtagctatat tttctcaaaa aaagtttggg aaggacttcc tgcatatgac
    22381 ttaaagactt tatgtagagt aaacgacatc cctctggtac accacagagc tggcgctgac
    22441 agcagagcaa ctgcagaatt agcattaaag gcttttgaaa ttgcgggtgt tatttcacta
    22501 gatgatttcc ctgagaaatt aaaaaccaca attggacagc tctatcacgg tggatacagt
    22561 ccatctgaga caaagagaat aaataaggca aaagatttat cgaaaatagt tggcgaccca
    22621 gcaaaacaca atgcaggcag tatcttttat ggcagaacag tcgtttttac aggaacttta
    22681 tcttcaatga taagaaccga agctcagcaa ataatagcag acattggtgg cataaatgga
    22741 aatagtgtta caaaagatac ggatttttta gtagttgggc agcaagatta cagagtcgtt
    22801 ggcgaagatg gtatgagtaa taaacaggaa aaggcagtta aacttattga aaaaggttcg
    22861 actcttgaaa taatatcaga agatgacttt ttaaagaatt tataaacaaa tgaggcgatc
    22921 gcataaaaaa cagttttttg aaccatcagc aatccctacc ccgttcatag ggatccgatc
    22981 actcccggct gagcctgccg ccggccagtc ggttactttc atcccggcga gatataacca
    23041 gcttatgaaa agaacctttt tacttttatt tataatttgt tcgttttctg ttgtttacgg
    23101 acaaaagaac attcgatcgg ataagaaaaa gataaaattt agtgttaacg gcgccgtgca
    23161 ggagtggcgt atttcaccgg aagataatcc ggacaggctg aaagtttatt gttcccatga
    23221 gaagaatacg gtgatatttc aaaccaatat tgacaccgct ttattttatg tttccaaaaa
    23281 cgacaccata aaattcaata tcattcttaa ccatcaggac actgcgcata ctgagattgt
    23341 cggaatcaag gatttgccgg acaaaattcc cgccgatgaa aagttgtact ggctgagcca
    23401 gatctggtca gaaacgaagt acaattttgt aaacattgac cggcttaaac ctgatccgga
    23461 tagcctttac aggactttta tccctctggc gctggcaacc aaaaacgact atgaatacta
    23521 tcaggtattg aaaaagttca tggcaaattt acacgatggt cacagcgagg tatccgataa
    23581 tggacaattt cgtccttata ccgattatat cccggtctct ttgcaaaact ttaataacaa
    23641 agtctatata acctctgtgc gtaaaatacc cgaacttgat tcaacctggg ttggcgccga
    23701 ggtcattgaa attgagaata ttcctacatt aaagtatctc gaaacaaata tatttccata
    23761 catatccgct tctaccgaac aacatttatg gatgcaggga atggttaagc tgccgaatgg
    23821 cttcaaagat cagccgttca aagggaaaat cagaaaaacc gatggcggta ttgttaatat
    23881 tgagcttcag cacaacggcg aggctacccg caccgatcat gatcaatctt gggggccggc
    23941 aataaattac ccgcaagata ttgttgatct gaaatggctg gataaggata ttgcattggt
    24001 aagtttcaat cgattttggc cggaagagga agcgatcaaa gaatttgaca atgtagttga
    24061 caagttatca aaagcaaaag gggtaataat tgaccttcgt aaaaacggcg gcggaaccac
    24121 caatgtagcc tggcatttgc aaaaatacct gacaaagggg aattattttc tcaactttgc
    24181 ctcggagaca agaataaatg acggagtaag aaaagcaaac gggaactggc aggaacaata
    24241 taaagattac tttttaaatc gtgcatatcg tttcgagagg cctgattctg tatttgtatc
    24301 agatacacta aaaaggatca cttgtccgac ggttatatta atcggacggt ttactttttc
    24361 tgcggccgaa gatttcctgg ttaacatata tgaaacaccg gagaggccaa tactgattgg
    24421 tgaagaaacc ggcggctcaa ccggttcccc tttggtggta cagggattac cggggggagg
    24481 atatgccaga atttgtacaa gacgcatctg ctacccgatc agtgaaaaaa gatttgtaaa
    24541 tagtggcgtt aagccggata ttgaagtgaa agaaacgatc gacgattaca tgaagaatag
    24601 agatgcagcg ctggaacgtg cgatcaaaga aattgaaaac gccctgtaat atcagctacc
    24661 cgtcaatggc ggcggcagtg gttttcggtg ggcatctttc ggctcaggcc gtttttcggc
    24721 ttgacatccg gcagttggca tgaccgatct gcaagccctt ccccgttcat caaaaaatcg
    24781 tcgatcactc ccggctgagc cagtccgaca aaacaaatct tatccttgtt aaaagatgta
    24841 aaaaggagca tccggatata cgatggacac agcgtttcat taattatata aaagctttta
    24901 gccaactcga acgctttgtt gatgttgata agtttaacga gttggaaaaa ctgggcctca
    24961 ttaaagcctt tgagtataca tttgagttag gctgggaaac gcttcaggat ttactgaaag
    25021 aaaaaggtta ttatggtatc atcgggccga aacctgttat tgaacaaagt tttcaggacg
    25081 gttatattac tgatggaaat ggctggatga gaatgcacaa aagccgtaat ctgacaagcc
    25141 acacttatga tgaagaaacc gctgaagaaa taatcaggaa tatcagatat gagtattttc
    25201 ttttattaaa ggatctgaaa ggagcgcttg aagacgaatt aaaaaaaata caatgatgac
    25261 ggtcacagat aaatacggtc taaatgagat tgaattagaa aatataatat ccgtctttaa
    25321 agaaaatgct aaaattgata aagtagtttt atttgggtcg agagcaatag gaaattactc
    25381 taccggctct gatattgaca tttcaataag cggaagagat ttattgctga acgacgttct
    25441 ggatttatca ataaaactgg acgaatttga ttttccctac aagtttgata ttctgattta
    25501 caatgatatt aaagaaaaag cgcttgttga gcatattgac agggtcggtg ttgtgattta
    25561 cagcagatat tcattcccag ataaatagaa ttttgaacca ttatcaaatt ctgtcccgga
    25621 aagggatccc gtcgatcact cccggctggg cctgcctccg gcgggcaggt tgcatttctc
    25681 caggcggggc gcattttatc agctataccg gttgtttttt aagtcaggct ggtataattt
    25741 tatgctttgg ttaactttgt tcttaccctc aaatcctgcc gcctatgcat ttccgtcttg
    25801 ttatgctgcc ggttatactg ctcttctggg catcctgcac cgaagaaccc gaagaaaaag
    25861 tccggattcc tgaaagtatc gttgccggtc agacgagcgg caacggaatc gtttatacaa
    25921 accctcagcc cgacgattcc ttttactttg ccgggctggc atcgcaagag gtattctatg
    25981 acctgaaccg tgacggcaac gatgaccttg ttttcagcat gaccgattat cccgattccg
    26041 tatccggcta ctattccgcc agcctgcggc cggtttaccc taatcagatt gtacgcaccc
    26101 cccagctcga ctactacctg cggcccctga atgccggtga taccatcggc aggaacagtt
    26161 actggcatga gggtagtgcg ttcatgttct atgaaggctg ggatcccggg catcattggt
    26221 ctacaggcca gtgggatact atccggcacg cttttgccgg cgtaaaactg atcacgggaa
    26281 ccgatacttc ctattgctgg atcagcctgt ttcattccaa ggagcctccg catgccctgg
    26341 tgatccggga ttatgcttat accatccgct atacggaacc ctgatgtaac cggctatttc
    26401 cgccatccgc tgaaaggccg gcatccggag gcgggcaggc tgtcccctgc aattgaagca
    26461 atacccatta ttaatgggag aaaggatgca atgaggatcc ctttccggca gtaactttat
    26521 gcaatgtcgc atgcctgggc ggttattgtt gcccgtcagg tttcaggatg ctcaggtcaa
    26581 cggtccgcag ccgccagttt tcgcccagga tgaacctccg gtttttaaat gctgacgcca
    26641 ccaagccggc aaaaatcaca gacccggcgg cagaaacgac gtccggcccc agggccggcc
    26701 ctcccgaacc gattaccccg ttaaccagtg taagtcccag gaaaccggcg ccgcaaatta
    26761 tggccacatt ggagaaaatc cgcacaaggg gccgtccgcg aaaaaccgcc gtaatatcac
    26821 taaacctgta acggttattc ccgttgatct gtactgtgga atccgtaatt tccgtaatca
    26881 cacccgcatc aaaaaagccc tcgggttttg tcttataccg gatggaaatg aaatcgtgct
    26941 tctgatacat gtaataggta accgtgcccg gcttttcgag aagcagggtt atctgggctg
    27001 aagatggcaa tccgatgaaa accagggcta acgaaaagca gatgacaagt tttttcattt
    27061 cgggatgatt tataaatacc aacctgggtc tataaaacaa gttataaata ccggaaaata
    27121 ctccggtgca aaaaccgccg gagccggcgc cgcgcgacta ccggcctgcc aggtttaaga
    27181 ccgcaggtta tggcgtttca accggcatta cccataacaa agataatgaa tcattagcga
    27241 ccggcggaac aggtttatga attgtagcat aaattactcc tgccccacag ggcctctccc
    27301 gcatttttat agcatttgta agttgggagg aaaagttcgt tcctgcattt gtcaggttta
    27361 cgggttcatt cccatcagcc ccggagggat tcttttaaag ccctgatgtc atcaatgagg
    27421 cccttaaaca gatggttacg ttcctttgcc gaagtaccgc tgatgagcac gccggattgc
    27481 atggtgttcc gcaacgattg ctctatctgc aggcagattt ccgcatcctg tgttgccagt
    27541 aaattaagcc cgttgtaagt aagataggcc gtcatcctgt gatcggtatt gatgaggatg
    27601 gtactgtcga ccaggaaaac atcgttgtag taaacgctga aggaattatc aattccggag
    27661 tgctcgcttc cataagggaa attatacccg gcctctgcct ggatttttaa atggctatag
    27721 agctgctcca acagttcgca gagatatata gcatccctca catcggcaaa aaagcccgat
    27781 tcccagcaat aggaaatttg cctcagggtg cactgaagcg tttcgttgca ccagatttca
    27841 tatttcggaa tatggaggta ataagcgtat atttcctgat ttaaaccgag cagaccgcta
    27901 taatcatcgg ctgttgagaa acgcctgtcc tggcgctccg ataaacggta ttgaatgcgg
    27961 ctccagaaga acagcttgaa tgccagcaat tccgggaacg gaataaaatg gaacagtgat
    28021 atttccttgg tacaaaaaat cacatcctta tactggcagt caagtacctt tttagaaacg
    28081 tctttaacta tggcagacaa cttggtataa taattaaaac tgtcgccctg taaatcaata
    28141 gagttaaata ccttatgccg gtgatcaatg ttgataaggg aatcgaatga aatgtcgtag
    28201 tggatacata gtcttcgcaa ttcttcgata gataagtcct tttcacccct cagacgacgg
    28261 tatgcgctgt cgtagctgat ccccaacaat tcagacaatt catggaccag cgacagattt
    28321 gcgggaagcc gcttcttcaa tacatcgaaa aacattaact gggtcgaaat cacctggttc
    28381 atctctgcct ttccttgaat tgtgatgatg caaatcagag cctgaggggg agcgggggaa
    28441 gtaatcataa taccagcata caataaaatt actacaattt tgccccggat gcaaaaagcg
    28501 gaattccggc attcgaaaag tagtttttat taagggtgat agccgctgca gccctgaata
    28561 accggcaatt ttcgccattt atcaggatag tttttacgcc cggcagtaat atcttcggaa
    28621 cactgaccgg tttgttcagc catgaaatga ttttagcaac aggatgcagg ctatcccctg
    28681 atttttgcag atattattac agccgccata tatcctttta tttatctttt ttgctttgcg
    28741 ttatacaggt tgtaaattca tacaaatttt ccagaaaatc aactaacatt cccggtattc
    28801 tctattgctc attccccggt acaggcagcg gcagaagtgc cttagttaat acagacagac
    28861 aaccatttaa attcaggacc atgaaaaagc caattagaat tttattaatg ctggcaacag
    28921 ggataattgc tttttccggt tgtcagaaag agacggatga cctgattcct gtgaatcaaa
    28981 acaaggctga atcagcattc aacgaatctt cacagccttt accaggcata agttggagca
    29041 acaatttcaa tacggaagca agtctgcttc aatcctttta tcttgtcggc agcccggcgc
    29101 ccgtttgggt tgacgaggca tgcggccgtc agggattgtt cgacaacaat gccacttcgc
    29161 caacggcagg atatgggatc tcaaaagtcc ccatcggcaa cggaggcccg gtcgtcatcg
    29221 aagctgaagt ttaccttaaa attaccgatg ctaacggata ctgcgtttgc ccgggcctgg
    29281 gaatcaccct caaagattat cagccgttgg tactttggta ggatatcccc ctcggaacgt
    29341 atttccgcat tatgtatgcc gggactcagg ccacctgggt acccgagaga tgcaggggcc
    29401 acgcctggat acaggccggc tataacaaca gttccggaaa agctaccaca accggtgaat
    29461 atgctatggt tgccgacagc tataccaatt cctggcataa gatcaaaatc atgatcaccc
    29521 aggatcactt cgttcgtttt tacatcgacg acgtcctgat ctggtctccc ggagataaga
    29581 tcagcagcca acagcttcag ggcaagcacc ttgtcctggg cttcacatca tccggtaatg
    29641 tcggcaaagc ttatcacaac tatgtaaagg tgacttaccc ggattattat tgagatgatt
    29701 cccatgatga aacgccagga tggagatacc tgatgctgcc tgtactttcg tgaattcctg
    29761 gctaaaagcg cctgccctct tttcaggggg caggtttttt ctgcctgcat attaaaatat
    29821 actatggcgt tcctataaac gttaagataa ttgaatagcc tctttcaatg attttacgat
    29881 ctttgtgatc gttaattttc ctcatatctg atctatggct gccttacgaa aatttctgcc
    29941 tgttgtgctt gttgcaataa tggcgttggg cttttcgtgc aacaatatga gccgcaagtc
    30001 gatggtcatt cagggacagt ttaccggtgt tccgggcaaa cggattgttc tttatgagtt
    30061 gttccccgac agcctttgta tccttgactc tgctgctacc ggggaagacg gcgaattcag
    30121 attcaggata aaatcgggcg atgcaggttt ttacctgctg aaactcgatg acaggaactt
    30181 tataaccctg tcatccgggc cgggagaaac gatcaaactg tcgggcgacg gcaataacct
    30241 gcagcatgaa tattcggttt ccggatcggc aggaagcatc ctgtatcatg atttcctgat
    30301 gttcaccaac cggaaccaat cgaagaccga ttcgctgggg gaaattttca taacgagccg
    30361 gccgaaggct gatttcggag aaataaagga gcagcttgat tcggcttatt atggcatctt
    30421 tggtgaacag caacagcggg taaggaattt ccttgaggca aatgccggga acctggtttc
    30481 cctgctggtt atcaaccgcc ggtttggtcc taatgaagtg gttacccctg ccaccgacct
    30541 cccccttttt atccggattg acagcctgtt atcggcgaat tatccgggga acaagcatgc
    30601 cgcttctttt cataggacgc tggaagcata cagggcgcag gtttccggga cgagtctccg
    30661 gaataaggcc atgcttcccg ggagcccgga gccagacctt ggcctgccgg atgcttcggg
    30721 aaacatcatc cgtttgacgt ctttatcagg caaaccgcac cttatatatt tctggggttc
    30781 gtggaatgcg ccaagccgta agatgaacgc cgggctcata cccatttata atgagttccg
    30841 gaaaaaagga ttcgagattt atgccatagc gttggatacg cagaaggaac cctggctcaa
    30901 agcctgtcgc ctggataagg ccacatggat tcatgtgaac gacatgaaag ggttggattc
    30961 gcctgctgcc agggcatgcg gcatcaccgg catcccggca gcgattctga tcggggccga
    31021 cggcctcgtc aaagcgcgga atattcagcc cgatgaactg aaaacctggt taatgacttt
    31081 ttacaaataa aaatcccttt aactcctgtt gaaactggca cgaccgatga ccgacagcaa
    31141 caaagcaatt tatttcgcca gcgattttca tttcggcatt cccgaccgcc gcggaagttt
    31201 aatacgtgag cggaagttta ttcaatggct cgacgaggta aaaaaagacg cttcagccat
    31261 ttacctgatg ggagacttgt ttgacttttg gtatgaattc aggactgtgg ttcctaaagg
    31321 gtacatccgt atgcttggca agttgggtga aatgaccgat gccggcatcg aagttcacct
    31381 gttccgaggc aaccatgacc tctgggcgtt ccggtacctg gaggatgaat tgggaattct
    31441 cctgcatcgc gccccgatga tcgtgcaact cggaaataaa aagtttttcc tggcccatgg
    31501 cgacgggtta ggccccggcg atcacagtta caaattttta aagatggtgt tcgaatgccg
    31561 attcaaccaa tggctgtacc gctggataca tccggatatc ggcacccggc tcggcgctta
    31621 tttttcgcgt cgcagccgcc ttacgaaaat gctgaaaggc gaaggcgaca tcaaaagaaa
    31681 ggtacctgtg gaaaaggaac ctatgatcat tttcagcagg gaaatggcac aaaaagatcc
    31741 ttcaatcgat tatttcatct tcgggcatgt tcatttcccg gatgtggtca gtatatcccc
    31801 gaaagcccag gccgttatcc tcggcgattg ggtgtcgaaa ttcacctacg cccgttttga
    31861 cggtgaaacg gttgaaatga aacagtactg tccccgttca gacaacagca taccggatac
    31921 cgcacgcccc gggcttcctg attacaggta agccgccgcc ttatagatca tgccataccc
    31981 tgtgatcctc aattccggct tccaacagca cggattgcct gtcaaaaaaa aagtaatggt
    32041 ccaatcttat ttatcaaagc attgttttat ggcacagtaa ttggtatcat tgcaatgcat
    32101 cagagacagc ccctgacaac cattcaccct aaacattcaa atatgaaaac caacctttat
    32161 ttgtttatct tcctggcagt tgcagcatcc ataacctggt ccggatgcgg aaaagaaggc
    32221 ccggcaggca aagacggcat cgacggcaac gccaatgtca tagcttccca atggtataag
    32281 ccggcaacct gggccggtaa aaccggcgac tggtactttg atatttcgtg caacgaaata
    32341 acggaagaca ttgtagaatc aggcgttatt cttgcatact gcagccttcc cggcgatatt
    32401 tacgatgcgc cggttaggcc catgccctgc tatgccattg gcgcaaactg ggatttcctg
    32461 ataccggact acggacagat cgagtttacc tccgacgccc tttatgtacc cggaacttcc
    32521 gattattact tcaggttcat ccttatcccg gcaagtaatt tcctgaaaac atcatccgga
    32581 acaaacgtta cggcagacga actccggaaa atgacgtacc acgaagtatg cacaaaatac
    32641 ggtatcaaag aatagaaagc cctttcgttt tgtaacttta tgagagctcc cgtgaacacc
    32701 gggagcttct ctttttatgg cctgcatcaa ttcccatccc cctgttcaac atctttgatt
    32761 acctttgaaa agactaaaaa ctacacctga tggatactat taaatcttac cttgaagcca
    32821 acaaagccag gttcctgcag gaacttttcg accttatccg aattccgtct atcagttccg
    32881 aaagcgaaca taaaaatgac atgaaactgg cagcagagta tattacggaa tcgctgctga
    32941 aagccggagc cgataaagcc ggggtttatc ccaccaccgg gcatcctgtg gtttacgggg
    33001 aaaaaatcac cgatcccgcc aaaccgacca tcctcgttta cggccactat gatgtgatgc
    33061 cggttgatcc ggttgaactt tggaaaactc ctccctttga accggttatc gccgatggaa
    33121 aaatctttgc gcgtggcgct gttgatgata aaggtcagct tttcatgcag gtgaaagcgt
    33181 ttgaatacct tgtaaaaaca ggacaattgc cctgcaatgt aaaattcatg ctcgaaggcg
    33241 aagaagagat gggctctccg gccctggcaa aatggctgcc cgaaaacaag gaaatgctgg
    33301 ctgccgatct gatcctggtg agcgatacca gcatgatcgc ccttgatatc cccagcatca
    33361 ccgtggggct gcgcgggctg gcctacatgg aagtggaagt cacaggccct gataaagacc
    33421 tgcattccgg tttgtacggc ggggctgtgg ccaatccggc caacgtcctt gccagcctca
    33481 ttgcttcgct gcacgacgaa aaccaccata ttaccattcc cggtttttac gacgacgtaa
    33541 cagaactttc agccgaagat cgcaacgacc tggcccgggc ccctttcaac cttgaagagt
    33601 ataaacggaa aattgatatc agcgagcccc tgtgtgaaaa gggatataca atacaggaat
    33661 gcaccggcat caggcctacg cttgatgtaa acggaatctg gggcggatac accggagaag
    33721 gctcaaaaac cgtgctgccg gccaaagctt cggctaaaat cagcatgcgc ctggtcccca
    33781 atcaggatcc ccacaaaatt gcccaattgt ttgcccgtca cttcgagagg atcgccccgc
    33841 ccagtgttaa aataagagta aaggaagatc acggcggttt cccctatgta tcgccaacgt
    33901 caacgcgggc ttaccgcgca gcaagcaaag cctatgaaac caccttcggc gtgaaacccg
    33961 tgcctgtacg cagcggcgga agcattccca tcattaccac cttcgagaaa acactcggga
    34021 tcaagagcat actgatggga ttcgggctcg ataccgatgc cgcacactcg cccaatgaaa
    34081 actttcagct cgagtgtttc tataaaggca ttgaaaccat cacctggttt taccgctatt
    34141 ttgccggcga cctgaaacaa gagattgaat aacagtccgg acgctccaga attataaccg
    34201 aagcgaatag gaaagccatc tatttccgga tggctttcat catataattg cctttctcac
    34261 cgctgatgtt caataggtat acccctgaag gccaacatgc ggtttcaatt tcatgatgaa
    34321 attccggttc atccccggta taaactatcc gcccgaagga atccctgatt tgtatcctga
    34381 atttaccctc actccggatg gagagttgat cggaaaaagg attttgaaag tgacagacag
    34441 ccggattttc atttttcagg attccggcat tcagtaccgt aaactgctgt tctcctgcaa
    34501 attgcgaacc aagaaaagcc tggtcaagcg cctggatgct ccagtaataa ttcccgtcgg
    34561 gcaggttcac gacccaggaa gtatcatttg aaacgttgcc gggttgggaa atcagccggt
    34621 gtccgtcggc gctgttagcc atgcaggcaa ttatttcgtt gccaccggga gtggttccga
    34681 tccgcagatt gtaggtcagc gcctgcgtgg gggtatgatc atccgttgcc tggttccagg
    34741 ccagaagcac ctggtgtcct gaaactgttg atgttagtcc ggcaggagcc agcggcgggg
    34801 tattttcctg gaaaacatta ctgcctgcat tattccggta gagcgcagtg atcggcgagc
    34861 cggattcaga acttccggaa aggacaatat cgaggtcgcc gtcgttgtcg taatcgcccc
    34921 aggtggaaca gccacgctca acgcccgcca gtcccgctga aatattggtg aacatatacc
    34981 ccccgtcatt gcggtatatt accgatgaaa ggcttccgca ggcagggttc tggccggtaa
    35041 tcagcgcatc agcatccccg tcgttatcat agtcgcccca ggcaacagtc cccaatgatg
    35101 aatttaccat tcccggtatc atacaaaaaa actgcatccc tcccaaattt tgatagatga
    35161 gggtcttcgg ttcaaggtat tcatcaaatc cgtttaccag taagtcaagg tcgtgatcgt
    35221 tatcaacgtc gcaccacgag gcggttcctc cggaaagcgg cataattcct gttcccattg
    35281 cagtaaagaa gtcttcatcg ttacggtaaa tatccgaaac aagaacacct cccagatcgc
    35341 ccattacaaa gatgtcgagg tcgccgtcat tgtcgaaatc gccccaatca acccagccgt
    35401 tattgatggg cggcaattct gcgttaacat cggtaaatac tccgccattg ttattgaata
    35461 agcggcaact gaaattgcct gctatcaggg cgtcaagatc tccatcgccg tcatagtcgc
    35521 cccaggcaac agccccatca ctccctacag ccgggaaacc ggcattaacg gggacaaatt
    35581 caccggattt gttgcgaaac agccatgtat tcgaattgga agcgctgcgt cccgttgcca
    35641 ggatatcgag gtcgccgtcg ccatcaaagt cgccccagga tacggcgctg ttgtataccc
    35701 ccgtgaggtt ggcattgata tcggtaaaag tcccggcatc gttgcggtaa attttcatgg
    35761 tgtacgatcc gtttaccggg tcaccggaaa gaaggaggtc aaggtcgccg tcgttatcat
    35821 aatcgcccca ggccaacgaa ctgcggccaa cattgataaa tccgtcggac acggctgtga
    35881 acgattggcc ataggatacg ccggtgaacg ccagtagcaa aagagctgac aggaatgatg
    35941 tttttatcgt catgaggaat tgtagttgct gtgatcggaa ggttccaaaa gtaataaaat
    36001 gttgtaaaca attcatcagg aatacctctg ggtgcggcag ttgtacccga taaaaaaagc
    36061 gggtgcgggg aaaaccccgc acccgcatgc cttttttaaa agggatgatt accggtttct
    36121 gctgctgcgc gatgaacggc tcgatgtttt gtcagaggaa cggctccttt cggatttgtt
    36181 acctgtgtcg ctttttgcct gcttgcgttc aacttttgac gattccgtgg aacggccggc
    36241 tttctcttta cttctttctt ctgaagccga acgtttcact tctgcgttcc ggcggtcaga
    36301 tgattcgcgt ttcacggctt cccttgaagg ttcgctgctc cgtttattgt ccgacttaat
    36361 tgcgggctca cggtttccgg ctgccgacgg gccgttatca ttggacgcct tttcagttct
    36421 tttgcgatct gttgattctt tccctctttc actattcacc ggctgtgcat tgcgcttttg
    36481 ttcgttctta acattgttgc cgggttccac cctgccggga cgtgtctgaa tggcgtcata
    36541 gttgcgtttg ctgttggttg ccccctgtgg ttctgctccg gcttcccttc tgtggttaac
    36601 gccgggatcg ttggtacggg gattctgcgg cgtgatacgc gttccgctgc ggagattctc
    36661 tgcattgcgg tactcacccg ggcggctttc tccacggttg cggtcgacgc tggggcggta
    36721 catatcaacc tggccgtcgc gggtccgcag gttgtccggt ctgtcggtat ctacaactct
    36781 catcgtcctc accctggtat ttccgtacct ttccacatcg ctgacccgcg ggccatagaa
    36841 ccagttgtcg ttgcggtgca ttcccgggtt atcggtgtag taaacattgg tgatatgggt
    36901 gatatggttt atctgaaccg ggcggtcata gatgtaattg cgccagtgat gcgagcagaa
    36961 atggcggcgg ggtacaaagg tccaccaggg atcaggggca aaccagttga agtttacgct
    37021 cacgtaaatg tcgggaccca ttggcgccca gccgtaataa tcattgtatg atccccaggt
    37081 tacccatgca ggggcccatt cgaaaccggg tatccacatc cagccaagga aatcatcgaa
    37141 ataccatctt ccgtaatgga aagttgccca tccccagcgg tagtcggaaa tccaggtcca
    37201 gccgtattcg gtcaaagccc agttccctcc tgaagagtaa ggcctgaaag catcaggacg
    37261 atcgaagtat gggcgccaca cataaccgta gtcgggcgca tagatccact cgccataggg
    37321 cgaaagttca tcataaaaag tttgaaagga gatggttaca tgagtcggat aatatctgtg
    37381 agcttcgacg ctcagggttg cagccatcat caacatcagg gcaaaaatca gctttttcat
    37441 ggctttaggt tttagggtgt taatttctga ttttttgttt gttattgcct taccggcttg
    37501 tttatttaga aagaagcaac aggcgtgcca gaaatgaatt ttttaacgaa ataaatttat
    37561 ttctatagtt tatttcgctt tcagcatcat ttatattcct gaataagacg aatttatgag
    37621 aaatatcgtt acaaaaaatt cccggcagtt ttaccaacta ccgggaatct ttgaacagga
    37681 aatctcaaac cggttatttt ccggctgctt tcttgttgta tgcttctacg ccgttatcca
    37741 ggaatttgat aaactcattg atatccagat cataggcccg gggcgacacc agcatattgc
    37801 catcgccatc gagaattaca tagtagggct gagaattggt gccgaacctt tcgcgctgga
    37861 tttcagtatt tttcctcccc agaagcttta cttcagtgcc ttctttgttt ttgtaccagt
    37921 cttccttcgg catttcgatc actttatcgt cgacatacag cgccaccagt acaaagtttt
    37981 cgcgaagcct ctttaaaacc tgcggatcgc tccacacgcg gttttccatc tcacggcaat
    38041 tggtacagcc atggcctgta aaatcgacaa agaccggttt ctttaattct ttcgaaacct
    38101 ttagcgcctc gtcataatcg aaatagcctt tcaaaccatg cggcagtttc agctcagtat
    38161 ttgcatactt ggaggtttcg gcaagattgc cggcagcaac cgatccaccg ccgccggttc
    38221 ctgaattatc acgcacaatg gctgttacat caaaatcctg cgtatccata ggaggaagcc
    38281 agccggaaat gcctttgaga ggggctcccc ataaaccggg gatcagataa acgacgaagg
    38341 caaatgtggc aataatgagg acaagccgcg ggaatgattt taccacagga tagtcgctgt
    38401 cgtgcggaaa tcggattttt ccgagcaaat agaagcccag catggcaaag attacaatcc
    38461 agagcccgag gtaaacctcg cggtcaagga tgccccaatg gtaggtttga tcagggacat
    38521 tgaggaattt cagcgcaaag gcaagttcaa taaagccgag gacgactttt accgaattca
    38581 gccacccgcc cgatttaggc atcttattaa gccattgcgg gaaaaaggca aacagggtga
    38641 agggtatggc aaatgccagc gaaaacccaa gcatgcctat gacgggcttt aacacctccc
    38701 cgccaactgc ctgtactaca accgttccaa caattggtcc tgtacacgaa aaggaaacca
    38761 gcaccagggt aagagccata aatatggttc caaccacgcc gcctttttct tcgttctgca
    38821 cgctcttatt taccatccaa ctgggcatgg taatgtcgaa ataaccgaag aaggaagccg
    38881 caaataccac aaagatgatg aaaaacagca cattgggtat ccagtgcgta ctcagccaat
    38941 tgccgaaatc agcgccaaaa agtattgaaa taagcacccc gaatatcaca tagatggcga
    39001 tgatagaaag gccatagaca accgcctgta cttttccttt tgagccgctt ttcataaagt
    39061 aggataccgt catagggatc atcgggaaaa cacagggcgt cagaattgcc agcagccccc
    39121 aggcaaaagc ctggataaaa aaccaccaca gcgaacggtt cttcccggta aactcatcac
    39181 tgttgggccc ggcagcagca tctgcctttg ccaccgaatc gggagcggct gcaaccgtct
    39241 gctttgccga tgtatcgggc aaggcggttg ttgtttcaac agcctggtcc gaacctgctt
    39301 ttgcattttt ggcgccttcg agcgtgaaag aaaaagtctt ttcatcagag atgcattgct
    39361 tgtcgtcgca cgtctggtaa tacagaatac cgctgatggc tgcaggttta ttgccggtga
    39421 ttttcacccg ttgcgtaaaa acagccttca tatcatagga acggatcttt tgaccgaaca
    39481 tttcgtcata tatctcgtgc cctttgggtt ctttaacact cccctgcaac acggcgcctg
    39541 ttttcttttc aaatttaaat tcggttggca aagggccttc tcctacagga tacttttttt
    39601 gagaatacag gtgccatccc ggttcaatcg ttgcaacgaa attcagctcg gcaatggtat
    39661 cattgaccct gttggtttta taagtccact tcaccggttc caggatctgc gctgttaccg
    39721 gttttattcc cgccgtaacg gccaggaaga gcaccgcgac tactgcttgt aaggttagtt
    39781 tagacattcg agacatttaa caggtgtatt aatttgtaac gtacttatta tgtgttgttt
    39841 gtataaacat agggattctt caggcctacc ggctgcacat gtgagaggtt caggattggt
    39901 cgtttattac agcgataaaa atgttttgcg tgcgtttggt gattttgaaa cgatcgtcgg
    39961 tacgcacccc caggacccag ataatgttgc ttcccgaaca taaaagccac cgtttacttt
    40021 tgtccgattg tggcagtttc atgtcgataa acaggtcgct gagttttttg gttcctttca
    40081 tcccgaaagg gaccatacgg tcgccctcgc gccaatgccg gagtgtgagc gggaattcga
    40141 gcctgtcata atcaaaacag gcgatttcct tgtcatgagg cagtttaaac ccttcagctt
    40201 ttgcacaggt gaagtcaaca ggaacaggag ccgttgaaat aaattcattg ccatgctgtt
    40261 caaccgggaa ttcgatatca cccatatcgg caattttcgc tgtttcttca atcacaagaa
    40321 aatcgcgttc aaccgtcagg cggtaaaccg gcgaaaaaaa ggcctttctt tctgttcccc
    40381 ccagcgaacg ggcgatttcc ttcgttacgg tttccgagaa tccgtaagga cgtaatgctt
    40441 caaacagcca ggtttcagca ggttcaagtt ttcggaggct gccgatgggg atccggtcgc
    40501 ggtctttctc atgttccaca agggaagaaa tgccttccgc cagcttttgt ttatataccg
    40561 attccgaccc cctcaatctc cggatggtat catccaggtt ctgaacaaac ccggggttta
    40621 atttttcgaa taccggaagg agttcccacc ttacctgatt ccgaagatac ttggtgcttg
    40681 catttgaaga atcttcacgc catacgatgt tctgcgaatg agcgaaatcc aggatctcgc
    40741 cgcgggtagc aaacaacaga ggacgcaccg tcctgccctg cagcggcaaa atgccgtgca
    40801 ggccagcaat gcctgttccc cggacaagat tgataaagaa agtttcggcc tgatcattca
    40861 ggtgatgggc tgtggcgata taatcgtatc ccttttcacg gcatatttct tcgaaccagg
    40921 cataacgcaa gtccctggca gccatctgaa tggaaattcc ctgactgtgt gcaacctgtt
    40981 ttgtatcgaa acgtattgca aaaaaaggga ccttgtaacc ggcggcggcg gtttccacaa
    41041 atttttcatc ggcatcagac tcatcacccc tgaggttgaa attgcaatgg gcaattccga
    41101 atttgaagcc agcctcaaac atgagccggc acaacaccat ggagtcgatc cccccgctta
    41161 cggcaagcag gatattatct ttagcattga agagggattc agcttcgatg aaatgtaaaa
    41221 aacgcttgaa catattgtca aaagtacata ttattctatt tgggcaatac gataatccat
    41281 taaaatctta attatttagt ctgccaatta ctgtaccata aaaattgcag cgttaaattg
    41341 tcaaataatt ccgtaggttt gtaatcaaat atgcaaacct gaacaacggc tgacatgaaa
    41401 attgcagaga acggacaacg gcttacgatt tatattggtg aaagggatat gcagggcgat
    41461 atcaccctct gtgaaaccat cgtgctgaaa gccagggagc taaacatggc cggcgctact
    41521 gttattcacg ggtttatggg ctatggttcc agttgtcgcc tgcacagttc tctcgtcacc
    41581 catttcagta ataatctgcc gctggtaatc gagatcatcg acaaaccttc aaagataagc
    41641 ctgatactgc ccttcctgga agaagtcctg gatgaagggc tttacacggt tgaagatgtg
    41701 aaagcggtgt ggtgtaaaaa ggggtaagaa caggcaggag ttgcagcaga tattcgttgg
    41761 attttccatc aggcggggaa caggatcatt gaaacaggta aagatcaaac ccggttgcgc
    41821 aatcaccttt atcttaaaga agtatttgtt tgattcatat tgatattgcc tgaggatttt
    41881 ttttaatcaa ctatctgcct gatgcttaca aaaacagtaa taattaaatt tcttcaataa
    41941 aacactgccc gcatgagaaa tatcctatta ttcctgacat cccttttcgt tttatcaggg
    42001 ccactctgcg cccaggataa tgaagaaaat tataaaagcc ttgaatttca taccatgcct
    42061 tttaccctgt tggatctttc tccgcggttc aggcttgggg cagagtaccg gataacccag
    42121 agatttgcct gtgtacttga ttgtggcttt ggcaacaatg atctcaacgg atgggtgctc
    42181 agggaaactg tctggaaaaa tgattattcc ttcattgaag tgcgtccgga gctgaaatat
    42241 tattttccca aagccgacga tatcacttca ttttatgttg caacagagtt gttttatatc
    42301 cgaaccaaca gcatcctgaa ggatggatac tatcacccgg aaaaccagcc cttcgttatt
    42361 tgttatgagc tggcgacttt caacaggcag aaagcaggct tgcatatcaa aggaggggtt
    42421 acgctctctt tcagccgctt tgattttgat ttttacgggg gactgggaat cgcttcccgc
    42481 gagataaatt acagccatat tacaaatcct gaaacagaag aagaggatat attttttgaa
    42541 gaagggttca acaacagcta taaatatgag ggaggatcca tactgcctca tttaacttta
    42601 ggatgtaaaa tcggaatggt attgtggaag gattgacgga agccattcgg gggcagtccc
    42661 tggtttcccg tgacggaagt atataaaggt aaagccggaa ttccggtgaa tccatcacct
    42721 aaactccggc tttacgaaaa gcgaataatc acttttttac tttatctccc aggtctcgcc
    42781 gctgttgagc aggtcgtcga cgcattttat tttggaagta gccttggctg tgctgatctg
    42841 gtcttccaca aggtcgtcgt atgtggggta catggctgat ctgattacgc caaatgccat
    42901 ggggaaatga ggaggcgcca tttttgccag catcaggtgg atgcccggat cctgctggta
    42961 ctggtcgtga acgagaaggt cgtcttcggt gatgctgttt tcgcccaacg ttaccacttc
    43021 gaggcgtgta cctttcagcc tgattccttt atcgcggttt ttgccgaaaa tcatcggttc
    43081 tccgttcttg aggtggagct ggacatcatc gcggatttct ttatcggtaa cagcggcatg
    43141 ggttttatcg gcgaagatca cgcagttctg caatacttca atcaccgatg taccgtcatg
    43201 cttggcagct tcgaacatca cctcggtcat catttttaca ttattgtcca caacgcgggc
    43261 gaagaaagtc ccctgcgatc ccagtaccag ttcacccggg ttaaacggat gctcgatggt
    43321 tccctggggc gaggttttgg taatgctgcc catgggcgtt gttggcgaat attgtccctt
    43381 ggtcaggccg taaatctggt tgttgaacag gatgatgtta acgtcgatgt tgcggcggat
    43441 aatgtggata aagtggttac ccccaatggc catggaatcg ccgtcgccgg tagtaatcca
    43501 aacgctgagg cggggattgg caattttaac gccggaggcg atggcattgg cccgtccgtg
    43561 aattccgtga attccgtatg tattgacgta atatgggaaa cgcgatgagc aaccgattcc
    43621 ggataccatc ataaaatttt ctttacggta tccgattttg gggaacacgt tggccacggc
    43681 agcaagaatg gcatgggaac cgcatcccgg gcaccacttt accatctggt cgctaacaaa
    43741 atcttctttg ctcagttcta cggacgaacg ttctatagtt ttttccggaa attctatcat
    43801 gattatttct cctccagtat ttcgttaaac ttggcagtaa gctcggcaac cgtaaaaggc
    43861 aacgattgca atttattata ctgaatatag ctaaagtcag gttctttcat acgcaggtaa
    43921 ttcacaaatt gtcctgcgtt gagttcgcaa accacaattt tcctgaatcc tgaaagtacc
    43981 tggcgggtat ttttgggtaa cggcatgatg tagttgaaat gggcgaggct tactttttta
    44041 ccggcagcct gcattgcttc aacagcatcc tgggtggtac cgcgcgttcc gccccagctt
    44101 atcaccagca ggtcgccttc tttttcacca ataatttcct gttcgggaag aacatcggca
    44161 actttctgga ctttggcttc gcggagtttc accatgagtt catggttcag gggatcggta
    44221 ctcacgttac ctgtgatatt ttccttttcg aggccgccaa ccctgtgacg taaaccggca
    44281 gtacccggaa gggcccattt gcgcaccagt gtttcgggat tgcggaggta aggtttgtaa
    44341 gtggcgtcgt ttgcttcggc aatcggcgga gtgataggcg gcagatccga aacttttgta
    44401 atgcggaaaa gctgtgaacc atttcccagt gagccatcgc tgagcagaat taccggagtc
    44461 atgtgttcca tggcaatttt ggcggccatg taggcataaa aataacaatc ggccggagtt
    44521 gatgaagcca gcaccacaac gggagcttct ccgtttcgtc caaacagggc ctgcatcagg
    44581 tcgctctgtt cactcttggt aggcaggccg gtagaaggcc ccccgcgctg cacatcaacg
    44641 ataaccagcg gcagttcagt cattactgcc agaccaatgg cttcactctt gagcgataat
    44701 ccagggcccg aggtcgtggt tattgccatg gaaccggtat aacttgcacc aatggccgaa
    44761 caaattccgg ctatttcatc ttcagcctgg aatacccttg ccccaagcga tttatgcttt
    44821 gccagctcaa taagtatgtc ggtggcggga gtgataggat acgaccccag gaaaataggg
    44881 cgtcccgaac gttcggaggc tgccagcaat ccccaggcgg ttgctatatt cccggtgata
    44941 ttccggtatt tcccgggttt catcttcgct tttgaaatat ggtattttga agcaagggct
    45001 tcaatattgt cagcatagat gtatccggcc tcaagcacta ttttattggc ttcgatcatc
    45061 tcaggtttct ttttgaattt taccttaaaa taatcgatgg tggaatcgag tttccagcca
    45121 aacaagaaat agcaaatgcc cagggcgaac atgttacggg tgcgatcagc cgatttaacg
    45181 tcaaggttca gcggcttcaa tgctgcgcgg gtaagcgatg aaacaggcgc ttttacaaca
    45241 ttataacctg acaggctttc atcatccagg gggttggacg catatccggc tttcttaact
    45301 gaagcttcat caaaggcgtc agcgtcaagg ataagcgtag cgcccggatg ggcccatttg
    45361 aggtttgagc gaagcgaagc cggattcatt gcgacaagga tatcaacctg gtcacccgta
    45421 gtgtcaatct tttttgtgcc cacatggacc tggaatcccg atacaccggc agttgtattc
    45481 tgcggtgcgc ggatttcggc agggtaatcg gggaatgttg ccagatcaag gccctgaagt
    45541 gcagccgtat cgctgaacag ggttcccgaa agctgcatac catcgcccga atcgcctaca
    45601 aacttgatta caatgtcttc tttttcgata acatttgaat gatgactcat gctttccgat
    45661 attttttaaa tttgagcaaa gttagattta ttatgcggat atcaaggtga attacggtat
    45721 taaatttatc ggccactcac gcttcatctg cctgaaaaat cagctcagga cgggaataac
    45781 agcctgcgcc cggcaggctg aaacacttgc ggtgaaaaag gttagagccc atctgcgttt
    45841 catgaccgga atcaggaatc cggtgattat ttagaacaaa taaaaaatat tggtgtatct
    45901 ctccgtgaag aaaaaatgta tatacctttg ttccacataa cccaaaaaaa ttacaacaat
    45961 ggcttaccaa atttctgatg actgcaccgc ttgcggcacc tgtatcgatg agtgccccgt
    46021 ggaagctatc tccgaaggcg atatttataa aattgatccc gatatgtgca ccgaatgcgg
    46081 ttcgtgtgct gacgtatgcc ccgtagaagc catccatcct gcataagcat atccaggtaa
    46141 agcaattaca aaagggcctg ttcatcgcga acaggctttt ttcttacccg aatagccgtt
    46201 gtcagcatca tggctgttaa atttgacaat gaattcagat ccttctgaca aaaacaacgt
    46261 cccgttgcta atatccttta tcctggtaaa cccggcggtt cccgccaacg taggcgctgc
    46321 cgcacgggcc atgaaaacca tgggcttcag cgacctgagg cttgtcaatc cctgcaacca
    46381 cctgtgcccg gaagccagaa tgctcgccca tgcatccaac gaaattcttg aaaaagccgg
    46441 cgtatttccg tcgcttcacg aagctgccgc cgaccttgac ttcatcatcg gcgccacagc
    46501 ccgccgccgg acaaaaagag gtctgtatgt ccgctcaaac ctgctggcac aggtaattgc
    46561 agaaaaagga aacaccatta aacacacagg aattgtcttc ggaagcgaag agagcggttt
    46621 aagcaacgac gatgcatccg tttgccacct cctgacaagc gtttccatgg ccggaaaata
    46681 cccatcgctg aaccttgccc aggctgttat ggtttatgct tatgaactat cggcgctgaa
    46741 gggcagaaaa ttgcaggcaa aaaaagccgg atttgatact gacggtttgc aggcattgaa
    46801 aaccaaaaca gaaaccttgc ttgacgaaat cggcatcgcc gtcgacaaca gcaaccgcat
    46861 ccaccttata gagcgattag gccttctcgg cgaaaaagat gttcacctgc tgcatgccat
    46921 cgtcagtcag attaataaaa agctaaaata aatcatccgg tagcacagga caggtaaatc
    46981 atgccggtac gctggcattg acaactaact ttctttctcc ttcaccccaa tccttgccct
    47041 gaaaatttcc tttccgtctc tttcccgctt gccgtttata taaaaacggg attcgccggc
    47101 cggcgatgaa aaaaggccta tcctctcgcc ggtgaatgct tcggtgacga attctatctg
    47161 ataactttca attttatacc tgttattcca gtccggtccc agggcattta aaatccatgc
    47221 agcgtagcgg gtgttgttaa catgcccgag gcggtccgtt tccaggtact gaacagtatg
    47281 attgcccaaa ggctccaggg tttcaggcag gcgcaaccgt tccggtttat actcatccaa
    47341 tggcggctcc aggataaggt cgcggacaac ttcgggaatc tgaggctgcc gggtttgggg
    47401 attaataatg atccaccagg aagccccgct tccaagcgtc tgaccgttgg tgtccaggat
    47461 aaggaaatca cgtcgggcga aaattttctc gacgcccgta ggccaggttt taacgatgac
    47521 cgattccctc caggccgggt accggtcaat cttcatggta attcgcgcca gtacccatgc
    47581 gcggtctgtg tttttcaatt tctggaatcc gaagcccagg tggtcggcat gatgccaggc
    47641 cgactcctga aagtagttgc acatggcttc caggctggca aagcccgtgg caaggcaatc
    47701 gttccactgc actgaatact ggtcaaccca cgatgtcagt aattccgatg tatccatcat
    47761 gccgcaaaat tacaaaaagg cagtaaaccg gtatgccatg ataaatatag cgaaccggca
    47821 gccgatctta aaaaaggggg atgaattgta ataccatccg gcgtatctgc aaattgtagt
    47881 acttttgtgg agtttatttc actgcatcta tgagaaagta tgtagatttc ctggttatcg
    47941 gatcaggcat tgcaggatta agttatgctt taaaagccgc cggcaaagga acagtttgca
    48001 ttatcacaaa aaatagtgct gaagaaacgg ctacaaaata tgctcaggga gggattgccg
    48061 ccgttaccta tactcccgat acttacgaaa agcatatcca ggatacgctt atcgccggtg
    48121 atgagttaaa tgatgaaaaa atcgtccggc tgaccattac ggaagcccct gaacgtatca
    48181 gggaactcgt ggaatggggc gttgaatttg ataaaacagc gtcgggaaag tatgacctgg
    48241 ccagggaagg cgggcattcc gaatttcgcg tcctgcatta caaagaccag accgggcttg
    48301 agatcgaaag gaccctgctg aggcaggcgc gggaaaatcc caatatcgaa atactcgaaa
    48361 accatttcac cgtggatttg ctcacccagc accaccttgg tatggaagtg aaccgcagca
    48421 cccccgacct tacctgttac ggggcgtatg tattgaataa aaacaataac cggattgata
    48481 ccatcctggc aaaaacaacg ctgatggcta ccggcggggc cggcaacgta tatgccaaca
    48541 ctaccaatcc ccggactgct accggcgatg gtattgcaat ggttcaccgc gcaaagggcg
    48601 tagttgaaaa aatggagttc atacagttcc atcctaccgc tctttactat ccggccgaaa
    48661 aacccgcttt ccttatcagt gaagcattac gcggcttcgg cgctgtactc aaggaccgta
    48721 acggcaacga gttcatgcag aagtatgaca aacggatgtc gctggccccc cgcgatattg
    48781 tggcccgcgc catcgacaac gagcttaaga taagcggcga agatcacatt taccttgatt
    48841 gccggcatat caataaagaa gacctgctat cgcatttccc cggcatttat gccaaatgtc
    48901 tcagtgtggg catcgacatt tcgaaagaga tgattccggt tgttccctcg gcccattata
    48961 tctgcggcgg aattaaagta gatgaattcg ggcggagctc tatccggaac ctttacgctg
    49021 caggagaatg ttcctcaacc ggcttacacg gcgcaaaccg ccttgcatcc aattcactgc
    49081 tcgaagcgtt ggtgttttca caccgcgctg tgacggatgc atcatccaaa atcaagacaa
    49141 tcgcttttaa agacgaaatt cccgactggg atgccgaagg cacggtcatg accgaagaaa
    49201 tggtgctgat cacccagacg atcaaggagt tgcaggctat tatgtcgaat tacgtaggca
    49261 tcgtcaggtc gaacctgcgg ttgcaacgtg ccgccgacag gcttgccatc ctgagacgtg
    49321 aaacggacgc cctgtacaag caatcggtga tcatcgaaaa actttgcgag gtccgcaacc
    49381 tgataaccgt agccgggctg attatccgca tggcgcagga gcgtaaagaa agccgcgggc
    49441 tccatttttc aatcgactat cccaaatcga acgagaaaga ggagatgttt ttcaaccacg
    49501 gagacgaatt gtaaaataag gcagcgggga taaatccgca aaacggaatt gaacatcgga
    49561 aaaaatagcc atcgcttatg ccgctgatct atcccataaa aggaatttca ccacgcctgg
    49621 gcgccaatgt attcatagcc gaaaatgcca ccatcgtcgg cgatgttgaa atgggcaacg
    49681 actgcagcgt ttggtttaat gccgttgtaa ggggtgatgt tcattttatc aggatcggga
    49741 ataatgtcaa catccaggac ggggtggtca tacattgcac ctaccggaag tacccggtga
    49801 caatcggcaa taatgtttcc attgcgcaca gggctatcgt tcacggctgt acaatccacg
    49861 acaatgtgct tatcgggatg ggggccatta tcatggatgg tgttgtgatt gaaagcaact
    49921 ccatcgttgc tgcaggagcc gtggtatcgc agggcaccgt ggttgagcag ggaagcgttt
    49981 acgccggcat tccggcaaaa aaaatcaaat ccgtcgatac ttccctgttc gaaggccagg
    50041 taaaaaggat atccgacaat tacacccttt atgcgagctg gcatgaccct tcgatcgttc
    50101 cggccgggta taagggacag gatgaaagta acgaagaccg tgtatagacc gtaatccgga
    50161 aactctctta cggttaccct accggtctgc tccccgcctc tgacttttaa ccctgccgtc
    50221 ctcccacagg cccgccccag gcaggtaaac tttgcccttt ggcttttagc ttttagccat
    50281 tagccattag ctctctgctc cctactcctt gctccgttct ccctgctccc cgctcctcgc
    50341 tccatgctct ttgacctttg acctttgccc tttgactttt gacttttaac ctttgcccct
    50401 tgcccctttc tccctgctcc ctgctccctg ctccctgctc ccagcctctt ctccctgaca
    50461 ttctgacaca aaataaattc cggcaggaat cttgatataa ataaaagcac ttaaacaaaa
    50521 accaatagcg acatgtacat cctcattttc atcgttttca tgatcctgag ccttattatc
    50581 agcgaggtat tgaaatcgaa attcagaaag tactcaaaaa tacctgtaag caggggtctg
    50641 acgggagccg acgtggccga gaagatgctc cgcgaacaag gcgtattcga tgttaaagtt
    50701 gtttccgttg aaggacagct taccgaccat tataacccac agaccaaaac cgtgaacctg
    50761 agccgcgacg tttattacgg caacagtatt tctgctgcag ccgttgcagc ccacgaatgc
    50821 ggccatgccg tgcagcatgc cacccgttac ggttggctgc agatgcgttc aacgctggtg
    50881 cctgtggtta gttttgcttc aaactgggtg caatgggttc tcctggcggg aatattgctc
    50941 atcaacagtt ttcctcaatt gctgttggcc gggattgctt tgtttgcttt aaccaccctc
    51001 ttcagtttca ttacgctgcc tgttgagatc aatgcaagcc aacgcgccat tgcctggctc
    51061 accaacagcg gtcttaccac ttacgaaacc cgcgataaag ccgaagacgc actccgctgg
    51121 gcagcttaca cctatgttgt agcagcgctt tcatcactcg ctacgcttgt atactacatc
    51181 atgatcttta cgggacgcag ggattaagca gaacggaaaa ccaaagggag ccggggttat
    51241 tcccggcttt ttttattgaa gtttatccat gaattactgc ccgcgctgaa aactgcacaa
    51301 aacagcctta cctttgttca tggtcgggag aagcccgccg gcactccgaa cagcaaccat
    51361 catccaacat ttaaagcaca tgaaaataca ccttaccgtt atcagcgtac tgtgcttttt
    51421 aatatcggcg caaacgcgcg cccagatttg cggatgcacc gattcgctgg ccatcaatta
    51481 taatcccgcg gcaaccctta acgacggcag ttgtatctat aattcagcaa gcatcgctcc
    51541 cctttcatcc gtcaccttgc caggtgtggt ttcggaaaca tccggattgg cagcctggaa
    51601 cggatgcctc tggacgcaca acgataattc ggatacccgt ctctatgcac tggataccgc
    51661 aagcggcgcc atcatcgaca cggtccagct caacaacgtt gtaaataccg actgggagga
    51721 aatatcacag gatggcgatt acctttatat cggcgatttc ggaaacaacc tgaacggcaa
    51781 caggaccgat ttaaagatct taagaatcag caagagttcc attcttacca atgatccgat
    51841 cattgatacc atcaattttg cctattctga ccagcttgat tttacccccg ccggcgccaa
    51901 taatacggat ttcgattgcg aagcgatggt tgtatcggcc gatagcattt atttgtttac
    51961 caagcaatgg atcagcaaag gaacgatggt ttactcgttg ccaaaaatcc ccggcacata
    52021 ttcagcatat ccggtttctt cctttgacat cggagggctg ataaccggag ccacctacct
    52081 tgaaaataaa aaggtggcca tgctatgcgg ttacagcact ttgctgcagc cttttacctg
    52141 gctgttatat gatttcagcg gacacaggtt tttcagcgga aacaagcgga aaatttctgt
    52201 ttcacttccc tttcaccaaa cagaagccat aacaacctgt gacgggctca atgtatatat
    52261 gaccaatgaa gctttcagtc agcctcccgt cattaacaat ccgcaacaat tacacatttt
    52321 tgatttcagc gcctaccttt cacaatatct aaacaatctg ataatgaacc tcgaaatccc
    52381 tgccggagag gaggaggttt tcctgtaccc caatccggcg gctggttttg tatgcctgcg
    52441 ttttgacgac agcctttcgg ataagaaatt cagcttatta aattcaaccg gttcggcagt
    52501 tttattcgga acgctgaaag gaaattctgc cataatcgat cttacaagca tccggagcgg
    52561 aatctacctt ttaaagatag aagaaagcaa ccggcgtgca gtgaaactta tcaggcgata
    52621 aatgtaacac cggcaggagg ctgccaaaaa cggaaaggtg atgcaggaag ccgttgcaaa
    52681 aaaggaaggg atgcaggcac caggtgcata aatgcaaaga ggccgccatt ggggcagcct
    52741 cttttaatat tatcgggcat agttactgag taatgccgag
//
LOCUS       DAOPYV010000013        49334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_566_length_49334_cov_13.529211, whole genome shotgun sequence.
ACCESSION   DAOPYV010000013 DAOPYV010000000
VERSION     DAOPYV010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 49334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49334
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_566_length_49334_cov_13.529211"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..577)
                     /gene="mreD"
                     /locus_tag="PKI35_02910"
     CDS             complement(59..577)
                     /gene="mreD"
                     /locus_tag="PKI35_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNX43361.1"
                     /translation="MNRVVFQNVIRFVLLLLLQVLLFNKINLYGYLNPFVYILFILLL
                     PFETPGWLLLALSFITGLTVDLFSGTPGLHTAATVFAGFARPAIIRLVGKHDESDSSK
                     FPTIRDMGLKWFVSYASIVVFLHHAALNYLDVFSFTEFFRTLLRVIVSSLFTVLFVIL
                     IEYIFVSHREKR"
     gene            complement(570..1409)
                     /gene="mreC"
                     /locus_tag="PKI35_02915"
     CDS             complement(570..1409)
                     /gene="mreC"
                     /locus_tag="PKI35_02915"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNX43362.1"
                     /translation="MRNILAFIVRYHFLLLFVVLEAVAITMFVNSTYYQQSAVLNAGN
                     NITGRFYTSASNVTGYFRLRRTNELLSIENSILRQGSGMSFLKNDTNTFWKNDTLYRQ
                     QYKYIVARVIHNSTNKRNNYIMLDKGRRFGIEKDMAVITPDGIAGTVVSVSENFAWVM
                     SVLNKYTRISARIQKNNQMGTVVWNGVDYSIGTLTDIPAHVKLSRGDTIVTSGFSNIF
                     PAGLMLGTIKDYRVEQGEHFYVIPFAFSVDFNSLDYVYVVKSLMKEEQDKLKETTEEQ
                     GNE"
     gene            complement(1441..2463)
                     /locus_tag="PKI35_02920"
     CDS             complement(1441..2463)
                     /locus_tag="PKI35_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNX43363.1"
                     /translation="MGLFSFLTKEIAMDLGTANSIIIYNDKVVVDEPSIIAIERTTGK
                     VIAVGKKAQMMHGKTHENIKTIRPLRDGVIADFQSAEFMIREFIKMIGTNRSLFPPAL
                     RMVICIPSGITEVEERAVKDSAEQAGAKEVRLIHEPMAAAIGIGIDVLEPTGNMIIDI
                     GGGTSEIAVIALGGIVNNKSIRIAGDDFNADIEEYMRKQHNINIGERSSEMIKIEVGA
                     ALTEIDNPPPDYPVHGRDMLTGIPKEIYVNYAEIAHALDKSISKIEAAVLNALEMTPP
                     ELAADIFKTGIYLAGGGSLLRGLDKRIHLKTKLPVHVADDPLHAVARGTGIALKNFDK
                     FTFLIK"
     gene            complement(2531..4057)
                     /gene="purH"
                     /locus_tag="PKI35_02925"
     CDS             complement(2531..4057)
                     /gene="purH"
                     /locus_tag="PKI35_02925"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNX43364.1"
                     /translation="MEELKPIRSALISVYHKEGIDAIARLLNSLGVVMYSTGGTYDFI
                     RSLGLPCTTVESLTTYPSIFGGRVKTLHPAVFGGILFRRENPGDVAERENYGIPPIDL
                     VIVDLYPFESTVASGASHNDIVEKIDIGGISLIRAAAKNYNDVVIVSSTDQYNELISL
                     LNEKKGLTSLADRYRFAARAFDISSHYDSAIFNYFNRDAGIQAFKVSSQTARTLRYGE
                     NPHQKGIFFGNLDDVFEQLHGKEISYNNLVDLESAISLIDEFAGPAFAVIKHTNACGL
                     ATRPVLLQAWTDALAADPVSAYGGVVATNRVIDETTAGEMNKLFMEIVIAPGYEKNAL
                     EILKSKKNRILLQRKNADFQATQFRSALNGVLVQDKDRFTETPEMLKVVTRNAPSASQ
                     VDDLLFANIAVKHLKSNAIVLVRNRQLLGSGCGMTSRVDALKHAISKAHEFGFSLEGA
                     VMASDAYFPFADSVECAFNAGVKAVIEPGGSVRDVDSIRFCDEHDMAMVFTGVRHFKH
                     "
     gene            4237..5487
                     /locus_tag="PKI35_02930"
     CDS             4237..5487
                     /locus_tag="PKI35_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX43365.1"
                     /translation="MLFLRLFRESYHLALQAISANKFRTFLSLMGITIGIFSVISVLT
                     VFDSIEITIRKNIESLGSNVLFIQKWPWSMGGDEYPWWKYMNRPEARVQEMDEILRRS
                     SLTDAAGYYVATNKNVKYEGNTFENATIVAVSHDYDKVQAFNIDEGRYFTPLESQLGR
                     PVAIVGNKITAEIIKDADPIGKRILIFGQKVEIIGTFKKEGNDTFGQSNDEWVMLPVN
                     FGRNFLDLNSDNIESAILVRSKPGIPNDALHDELTGIMRSVRKLKPSAEDNFAINEMS
                     IITQGFDKIFRVIALVGWIVGGFSLLVGGFGIANIMFVSVKERTAQIGIQKSLGAKNY
                     FILLQFLFEAVFLALMGGILGLLIIYLGTVIVSNAANMTLVLTSGNIILGLSVSAFIG
                     LVSGLFPAWAASRLDPVEAMRSTF"
     gene            5636..7402
                     /gene="recJ"
                     /locus_tag="PKI35_02935"
     CDS             5636..7402
                     /gene="recJ"
                     /locus_tag="PKI35_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNX43366.1"
                     /translation="MEKRWVFKENGNTELVRDLAASLCIDEPLAGLLVQRGVTSFEEA
                     RNFFRPDLQNLYDPFLLKDMDVAISRIEKARDAGEKILVYGDYDVDGTSAVALVYTFF
                     RKQGFENLDFYIPNRYSEGYGISFQSIDFAADNGFALVIALDCGIKAVEKVKYARDKG
                     IDFIICDHHRPGDELPPAVAVLDAKREDSVYPFNELSGCGVGFKLIQAYAKKTGIAFD
                     NLYEYLDLVAVSIASDIVPITGENRILAYFGLKLINSKPRPGIEAILNVANIIRREET
                     ENGPVTETGIFTRELTINDVVFVIGPRINAAGRIESGRNSVQLLISQSLADAESIARQ
                     INDFNITRRDLDSQITQEALDMIAADKNLQGLRSTVVFNESWHKGVIGIVASRLTELY
                     YRPTVVLTQSNGLITGSARSVKDFDIYDAIDACSDLLEHFGGHTYAAGLSLKPENLKP
                     FKERFEQIVASSIEDRMLVPEVEIDLILDLHQITPKFLRILKQFAPFGPGNMSPVFMT
                     PRTRDTGYARIVGKNHVKMTVFQESVRSDPFSSIAFQQGDKYNRIQKGLPMDICYHIE
                     ENEWNGNKSIQLNIKDIRFSAE"
     gene            complement(7504..8691)
                     /locus_tag="PKI35_02940"
     CDS             complement(7504..8691)
                     /locus_tag="PKI35_02940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNX43367.1"
                     /translation="MDYPRVILKPGKEVPVLRFHPWIFSGAIASVSENLQDGAIVEVL
                     GSNGKYLASGHFQDSSIAVKILTFERQPIDRQFWYKQVRKAYSLREGLNLVSNPDTNC
                     YRLVHNEGDGMPGLIVDVYDSVAVLQAHSAGMYQARQDLAEAVMQIYGGKLSGIYDKS
                     SESLFKNTGLRVQNGWLTGGSSDVIAAEHGCKFVVDIEGGQKTGFFLDQRENRKKAGL
                     YARGRKVLNMFSYTGGFSVYALKSGAELVHSVDSSAPACKLAEKNAAINGFGGDRHLV
                     FATDARQYLETMKQGEYDMIILDPPAFAKNTSSRHNAIRGYTSLNASAMKKIAAGGIL
                     FTFSCSQVVSREQFNAAVAAAAIESGRSVRVLEQLGQSGDHPINIFQPEGEYLKGLVL
                     RVD"
     gene            8861..9745
                     /locus_tag="PKI35_02945"
     CDS             8861..9745
                     /locus_tag="PKI35_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43368.1"
                     /translation="MKRTLFLLIIPALLLTTSCNKQKEEIARLKAKNDSLLTLKFTSD
                     TTVIEFVKAFNDIQANLDSIKQKEMIITKSTEGTTELKQNAKDQISSDINSIYLLLKK
                     NKEMVAALRAKLSKSDKRIVELETMIDNLNKQIESKDLEIADLKESLSKLDIKVKDLS
                     SQVDNLNATVDNLSSDNKAKQQAIDEKTTELNTAYYVIGTSSELKAKNIITREGGFIG
                     IGRTSNLKNLVNMDNFTKIDITKVHEIPIMKSKVTLITTHPSNSYKLEGEKPVEKLVI
                     TDPKAFWSISKYLVISAK"
     gene            complement(9898..10341)
                     /locus_tag="PKI35_02950"
     CDS             complement(9898..10341)
                     /locus_tag="PKI35_02950"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43369.1"
                     /translation="MRLYKTLVLILLASLAFACGPKKVIEEKYEDGSPKVVKYYEKVN
                     GERQVVREQQFYANKNKKMEGEFTKMKRSGVWKAWYENGNLWSEGDFKDGKRDGMGKV
                     YYENGKLYFEGNYSADNKVGIWRFYDANGKVIREVDYNKEKVLIE"
     gene            complement(10338..11684)
                     /locus_tag="PKI35_02955"
     CDS             complement(10338..11684)
                     /locus_tag="PKI35_02955"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNX43370.1"
                     /translation="MENVFTIRNAFIVNEGRKYNGWITIRDGVIAQIGESGLKEPVTG
                     KIFDAGGKLLIPGVIDCHVHFREPGATRKGDIFTESRAAAAGGVTSFMEMPNTVPQTT
                     TLDLLEEKCSLASAKSLVNYSFFLGATNDNIREIENANPGIVGGVKLFFGSSTGNMLV
                     DNAHVLEEIFERSPVPVVAHCEDEAMIRYNSALMKEKFGEEVPVSMHPLIRSSEACYK
                     SSEQAVKLAQKFNTRLHIAHVSTAAEMRLLENGLPLSQKRITAEACVHYLWFCDEDYQ
                     RLGTKIKVNPAIKTASDREELLRGLLNNKLDIVTTDHAPHLPDEKKQSYFKAPSGTPL
                     VQHSLPAMLEMCRLGKITLEKVVEKMCHAPADCYNIERRGYLRPGYFADLVIVDPQSP
                     WTVDPGNILYKCGWSVFEGITFNSQVTHTFVNGNLIYENGKFDHSFTGKRLTFNRK"
     gene            11769..12566
                     /locus_tag="PKI35_02960"
     CDS             11769..12566
                     /locus_tag="PKI35_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNX43371.1"
                     /translation="MYSRKLFLLSISFLIAFQVNAQIGGLSVSKLNSFCADAVDRHQL
                     IVEPGFSYTTSKKHWNNNSDLLRIYSTNDSLNAEAGLRFRFTYGVSDNLEIGVTVPAD
                     MSTSSWGVKYLLFQDEKYGFAAIGGVNYPFGKGAINKKDAENAIQGGLGAVFTWSPTE
                     TFSADVTLQAAKYIPEPEYSSEGYFSLSADAGYYLFNGNLLVAAGLGYQKYFSDNLKQ
                     YSFTLYPGIAINTGKNFCITLIHSFDLAGRNTDKQQITDVSFTLTLD"
     gene            complement(12892..13620)
                     /locus_tag="PKI35_02965"
     CDS             complement(12892..13620)
                     /locus_tag="PKI35_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNX43372.1"
                     /translation="MSDSLVIIPTYNEKENIAKMIRKVFSLSKPFDLLIVEDNSPDGT
                     GKIVHSLMPEYEGRLFILERKGKMGLGTAYIAGFKWALERDYNYIFEMDCDFSHNPDD
                     LLRLYDACASYGADMSIGSRYVKGVNVVNWPMGRVLMSYFASKYVRLVTRMPIYDTTA
                     GFKCYTRKVLETIDLDAVKFMGYAFQIEMKYTAWSLGFNLVEVPIIFTDRTEGTSKMS
                     SKIFKEAFFGVISLRFKKIGPRAA"
     gene            complement(13700..15895)
                     /locus_tag="PKI35_02970"
     CDS             complement(13700..15895)
                     /locus_tag="PKI35_02970"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNX43373.1"
                     /translation="MKRLASIVLMFFSLAVYGQTTIAEARLLSSGTTVTVSGIVINGD
                     ELGAIRYIQDSTAGIAVYGNSLGSVQAGQQITVTGTLKNYYNLLEISPVSSFTINSSG
                     NPLPVPLQITPSQLSESNESQLVQISNVAFDAGQGSSFDAGTYTFTSGGQQGAVYVRP
                     GHPLIGTSIPYNEISLTGICSQYQSDYQLLLRGQSDITGGIISVNITDLPRAENISTS
                     GLTVSWITDVAASSGIFYGNTPQLELGQLSSAGNTTYHSVDITGAQASELFYVKAFSA
                     SGTDTAFAPVRVFITQSLSPGWMKAYFTSNVDVSFASGPSAISLGTKVEDTLIAYINR
                     AGYTIDMAMYNFTLDRIPYALNQAVQRGVKVRIVYNSNTSNTSMQWVPSIPKLASPDG
                     SDYGIMHNKFFVFDSGSPNPADAIVWTGSTNMTGQQLSSDANNVIIINDQSLAKAYTL
                     EFEEMWGSQDMTPSAANARFGPYKKDNTPHQFIIGGKDVESYFSPSDGVNDRIIESIG
                     SASSNVDAAVFIITRTDIANAIQDRVNAGVEVNMIIDSESDLTNYPAVANVLHGLGTH
                     FTSDKYTSGLEHNKYVLIDRKDASSDPQVLTGSHNWSTSADTRNDENTLVIHDAGLVN
                     LYFQEFASRFMRNGGILAVQEIAAGASSLVCYPNPASDYVNIDMKDASAYIESISVYD
                     MSGVCRFSAGGMVRQSSAVLPVSELPAGVYLVRVNTTKGLCMLKMIRNN"
     gene            complement(15974..17017)
                     /locus_tag="PKI35_02975"
     CDS             complement(15974..17017)
                     /locus_tag="PKI35_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNX43374.1"
                     /translation="MMTNKKLLFLFAAGAFMLLAHIPSLAQQQKAKVATIAFYNLENL
                     FDTVNDTLINDEEFLPEGANKWTDERYQKKLANMSKVISQIGEEYIKGGPTLIGVSEI
                     ENRKVLEDLINMPALANSGYGIVHYDSPDYRGVDVGLLYRKHDFTVINTVSARLLMPG
                     HEHWRSRDQLVVTGLLEGDTISVIVNHWPSRGNLPPFRAEAARLTRSLADSLFRVSRN
                     AKIVIMGDLNDDPVDESVNVILGAEGREQKVKQKMLFNPMWKMYKEGIGSLAYRDSWN
                     LFDQTILSAPLLDKKSGSWYWLKTKVFNKPYLISQQGQYAGYPFRTYAGGAYEGGYSD
                     HLPVYTVIVKEVH"
     gene            17194..19908
                     /locus_tag="PKI35_02980"
     CDS             17194..19908
                     /locus_tag="PKI35_02980"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX43375.1"
                     /translation="MKFFNALVVFFLLSATAFAQSYSISGVVKEGLTDKPIIHATVGA
                     GSVTGYTNDDGSFRLHGIAGGKYTLIVTMAGYETLSVPVTVADGPADVGKLQMQPSRA
                     QGESGVAEITIGDDGDENNEAVSGLLHSSDDIFVNTSSYTFSSMFFRPRGYDSEYETV
                     LIDGIPFNDAETGRAVWGEWGGLNDVMRNREYINGLQPGNFSLGNLGGSSYITTRASK
                     QRKQTKLSYSLSNRSYTNRIMFTYSTGMMKNDWAVTVSGSRRWSEEGYVEGTFYDAYA
                     CFLGVEKKINSRHSLSFSGFASPTRRGMNSASVQEVYDLVGSNYYNANWGYQNGDKRN
                     ARIRTVNEPKLILSEYWTIDEKTTLTTSLSYSFGKTGTTGLNWYNSRDPRPDYYRNLP
                     NYMQDPTDTLNSVVQAAIIDAWKNDPSVSQIDWNRLYQTNYLANLEGKQARYILENNI
                     TAQNQWYLTSRINRQTGEHSNFSGGIELNHYKGSHYKILEDLLGGKYWVDIDQFSERD
                     FKTDKEKLQNDLNNPNRVIREGDRFGYNYDLLSNTANLWGLLELNYPHFDYYIGAAFG
                     GTQYWREGFMRNGRNPDNSYGVSEKQSTLTYDIKAGSTWKVTGRHFVTANAAYIMRPP
                     LLRDAYISPRTGADLTPGITDEKILSGDISYVARFPNFNARVTLYETFFKDYSKILRF
                     YADDFETYVNMALTGVDRIHQGIEFGAETKIIDNLKLVTVASFGNYRYTNRPKATINY
                     DNGSEPDTTETVYAKYFYLGGTPQFAGSVGLKYNKNYWFFDLDVNYYDRIWLDFNPMR
                     RTQAAIANLGEGDPLIELITEQEQLDGGFTVDASIGKSIRINYKYFVNINFSVNNILD
                     NTSLISGGYEQNRFDYTHEYLQNGIDKFPGKYYYSYGRTFFLNVGFRF"
     gene            19966..21801
                     /locus_tag="PKI35_02985"
     CDS             19966..21801
                     /locus_tag="PKI35_02985"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HNX43376.1"
                     /translation="MKKYISRTLLFGFLAGILTITACVKGDFDEPPIYIPHVDFESNT
                     SIETLKTMLNGTLTTITDDIIIKGKVVANDESGNLYKKIVIQDETAGIEVLLNAVNLY
                     NEYKPGQEVYIKCKGMYLGSYKGLIQLGYDYNGGIGQLPEVMINEHLYRDSLPGSVPE
                     PVQITPGDNVNNAKYLSMLVSVDNANFPEGGTTVWAPGDASTSHSLFVSGKELIVRTS
                     NYANFALDTIPAGYGKVIGILSIYNTDYQLTLRDAADLQGFSGETPISADFVFPATGL
                     SPVTSIDEKFDNIVASTDVAINGWTNKAAAGSRNWQGKTFQAEKYAQATSYNTTDAEN
                     KAWLITPPVVYSSSLKLSFSSEKAYYKHDGLSVWILTNYTGDPNTATWTKINATVAGS
                     ANADYEWVPSGDINLSDYVPANYTGNVYIGFEYTGNSTNTTTYCIDNILVGTGGGSGG
                     GTLLDEGFTTALGSFSAVSVTGSQAWGWADYDEGSAKMSGYASGSNHANEDWLISPQI
                     SLAGKTGVKMSFREAINYITSINDLKVLVSTNYSGSGDPNAATWNEVTGFTRAAGNNW
                     TFVDAGQASLSAYEGQNIHIGFKYVSSASAGATWEIGQVVVTSSK"
     gene            complement(21867..25316)
                     /gene="mfd"
                     /locus_tag="PKI35_02990"
     CDS             complement(21867..25316)
                     /gene="mfd"
                     /locus_tag="PKI35_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNX43377.1"
                     /translation="MDQDFETNDLIVNSQDTDSVTRLLSCYRSDERLVSLSALFNEAG
                     IARQRIHLNGLAGSSVSLVTAALFESNPVVHLVILSEKESAVYFCNDLENLLGEAETA
                     FHKKRVLLFPTSYKRPYEIEKTDNSNVLMRTEVLKRLSTRAKNTVVVTWPEALSEKVV
                     TKNYLEKSTLHLKCNEPVDLDFVYDMLDEYGFGRVDFVAEPGQMAIRGGIIDVFSFTN
                     DYPFRIEFSDEIVASIRSFDPATQLSIDKLDHITIVPNVQDRVLHEKRESFLEFIPGK
                     SILWFQDMAFAVDLIEKEYQKAKTAFEQLDGLVEHLGPEELFTGKESVIHGIGEFPVI
                     EFGRHHALTADASFGFDIVPQPSFNKNFDLLLDNLRENAENGIRNLLLADSPQQVERI
                     YSIIDDLLKNRGLNPEDINYASINLSLHEGFIDRGTKLACYTDHQIFERYHKFRLRES
                     FSGKQAITLKELYDLKPGDYVTHIDHGVGRYDGLEKIENNGKIQEAIRIIYANDDLLY
                     VSIHSLHRICKYSGKEGHIPSLDRLGSNSWNKLKAKTKAKVKDIAKDLIKLYAERRAG
                     KGFGFAPDTYLQHELEASFIYEDTPDQVKSTSDVKQDMEQDFPMDRLICGDVGFGKTE
                     IAIRAAFKAVNDSKQVAVLVPTTILALQHYKTFSERLREMPCRVDYINRFKSPQQQKK
                     TLAELQEGKIDVLIGTHRLLSADVKFKDLGLMIVDEEQKFGVAAKEKLKQMKVNVDTL
                     TLTATPIPRTLQFSLMGARDLSIINTPPPNRYPIQTELHAFNEEVIRDAVSYELSRGG
                     QVFFVNNRIQNIQEIAGVVQKLVPDARIAVGHGQMDGMKLEKIMADFVEGEYDVLVST
                     TIVESGLDIPNANTIIINDAHHYGLSDLHQLRGRVGRANRKAFCYLLAPPMSVLTDEA
                     RKRLKAIEEFSEIGSGFNIAMRDLDIRGAGNILGAEQSGFITEIGFEMYQKILDEAMQ
                     ELKHTEFHDLYAHEEEKEFVKECQVETDLEILIPDDYVTSNVERMSLYKELDGIEDED
                     LLLTFQEKMIDRFGPVPAETQELFNAIRMRRLARELGMEKMILRNGNMTCYFVSNQES
                     PYYQSDIFTQVLKYVQANAATCRMREAVGKLTLTFRNINTVMDALGVLRKIDGRDKIL
                     PAQ"
     gene            25550..25786
                     /locus_tag="PKI35_02995"
     CDS             25550..25786
                     /locus_tag="PKI35_02995"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNX43378.1"
                     /translation="MEPKIAQKGPFAVDLEEGQTYHWCACGRSQNQPFCDGSHEGTGF
                     TPVVFTPEKGGKAYLCGCKHSKNKPYCDGTHNTL"
     gene            25850..26200
                     /locus_tag="PKI35_03000"
     CDS             25850..26200
                     /locus_tag="PKI35_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43379.1"
                     /translation="MSVFIMTGNNPCIPIIRYSCLVNPDFIFHAMIISIEISYYPLHG
                     DDFIEPIKAFIDRLNSHPEMEVHTNGMSTQVFGEYDKVMEILQKEIRASFENPYSVFV
                     MKVVNADLRVHAKQ"
     gene            complement(26229..27776)
                     /locus_tag="PKI35_03005"
     CDS             complement(26229..27776)
                     /locus_tag="PKI35_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43380.1"
                     /translation="MNSKLLLVPVIAVSMTAMAQEPNSVAIYYNPLFGNPSGNVKCTT
                     CGLLEKDLTAVPGLKVFERQRIDVILQETTLGGSGLVTPETKAEGGKIMKAATIIQLS
                     GANISDGDVSADASLNALMIDSKSGRILSVLTAAFDETGSFSKQIASLSNSGFASPVA
                     VQKAVAPRRLTSVEVDIPVEYDFRYHGVFTDEEGKQEVRQSDQYVLYADGSKKQDKEN
                     KLYISSLRLLSGNFSNENFFATFESGIFTDEYISIEGKFNKDKSRIENIKFSRLSCDS
                     YDYGDNNLGYTKHDDMVWAENLVYNSVLKGYEYKNGVSNISKIKSTIDQYESGSVPER
                     TINISHRYKNIDGSRLDYDKKPLGPRVWLYSSEAVARKPVKKIYLRGNWKAYGFVAGL
                     LARQYPDAVLYDQTDIFKAITGNESKISETPPLVSNWNSPAKPAVDEAIVTFTTSGGN
                     DISAEVVTARQTGMVTTKNPELDFSGYAKPPSGEEQVVDPRVDLVRFYQLRWNILGSS
                     IIKEIEK"
     gene            complement(27971..28588)
                     /locus_tag="PKI35_03010"
     CDS             complement(27971..28588)
                     /locus_tag="PKI35_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43381.1"
                     /translation="MNTKLIAASFVALMLFGCNSNKTENSTAASSEGLFGKAAAIKVK
                     YDADYDALKEKAKTEVKSADDLVKINAEMNKMQETAKKEFSDAVASMKFPIDIPFVDS
                     TNGKSFKVQSVQVTEMQWDMAKVSAKVQVLIPVEKTMGGQVMPKIINALMVDKDGKPL
                     DAGQQGWFTLSSIKDYAVNDTVEFTNAFTDYVKVEKLAKLIIKDK"
     gene            complement(29062..30102)
                     /locus_tag="PKI35_03015"
     CDS             complement(29062..30102)
                     /locus_tag="PKI35_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43382.1"
                     /translation="MKYNFFYLVLLVITALWSKPLRAQDDMLLPDKPGTFRITKNKLN
                     GQDSDAYGKTFNFTDAEAAAASENLGSLIGIFRKTPVLSNIKGFDGVGLLNASSCFVK
                     FGYGIPCEVVFCFQTWKLMDGKEVKFTGEPPYWQFDVNRFDMFCSHGFNETNYRDTYD
                     ATNPGFTPKKMDDAALALHELFFLPDIREELSPGIDRYGNTVVIYNPDRPPYWEQVTI
                     REVFGLILDYWKVMPDKITSEIMIPVITKEFDHFSENEKDGFAYLSDKESITGIGSVK
                     NETPVMRPNPAYWNKSLPRSAIQIMVLDIPPKDIVKSGMEEELHRQSGYYYVKKLLYQ
                     LDPNSLLPAIAR"
     gene            complement(30147..32063)
                     /locus_tag="PKI35_03020"
     CDS             complement(30147..32063)
                     /locus_tag="PKI35_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43383.1"
                     /translation="MYKYVKVDTTKVISEAQMVKIATRFYDRHYKAVYAGSEKKFVDD
                     NFKKLETEGADRYKIAQGLSSTAVCLTGTGQAFNISLAFSAKAVILFPKDTLIVNNFG
                     AILRMMDSLKTSLPVLLYAKSLYPSAPVILTNLGNTLFELYDDRSAEAFYKRALKINP
                     DFALARQGLLNVYLKRKDFKNALEELYKGAKGIYCQSMKTVHENLKSRDDYKAPGEPG
                     GNKPGTDPSAGEGSGTPNPDVPVENLHLPSFPQWPDVSALIHDNSIKDLIKVLQEGRD
                     ESQEKSLGDFAGLADMTPEQQAAWARSFTKKETTPGLIRFDNTAFGMELLKAYFEDQI
                     AKENKRYIKSDSLANAKFMKSLEQIEFANDAKMKTLDGDVEKIQAFLIERCKEITNLV
                     DDNYKEWRDNAARRQNRYNDLLTTYWVYCEQYLNRTYDLNEFGKLNNARKAFVYSSYY
                     TLYYEYQVRQLLFASLNMTAFASVGGDCPSVPPPPPASQAEDNEVNVPDKKVPDCPFE
                     GKKLKVGLIVCSFGLDCESIEGECGEGLLAGAKWNYKKKEITGFFGAGVKADLGVEGG
                     VNVKAEGKTGFEITYNTKSQVSDLGFKSEATLTAGVGKMEVGQAFEAKVTAATGIDVG
                     RTTELTYNPLGFDK"
     gene            complement(32359..34449)
                     /locus_tag="PKI35_03025"
     CDS             complement(32359..34449)
                     /locus_tag="PKI35_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43384.1"
                     /translation="MNRFIYLVLLFAIAFPALSQNSSPDAIRKQMAKIRQTTNWNDPA
                     AAKKANEEIQKLARQMTGQPVKPAGNASQPTKTPGELLIKSPATEANVLAIASRFYAR
                     SYKALDAISKQQFDLDYKQAGYDKFSFNAVRKLTSAGATLTALGNDHNLACVYLTAAL
                     KARPTDTLSANNFGGYLRVIDSIEASVPVLLYANSLFGQSPVILTQLGCSYIELNDEK
                     TGENYLKQAIEYNPDFGQAHSALCDLYIKQNRLKEAIGQLFAGVKGIGVSYAKASNNY
                     AYMQKQAEDAGEAGNQSAKEDFWDETRNQLEPPDPLASLVPEDDRIKMPSFPNCDKVA
                     DWTEGGGYSSAVDAYGRFHAKLMAFSNEFLQVHTELPSLPPNAILRDYPNERFALDCI
                     TEYFFQESDKVDKRYQEKIDEIMKKDYDEINAYLEKHERLVKTYVGCVEGCGAEEHCI
                     EECHRKYCSEECPAANITNGKHQGYFEDSRKQFRDMVKEQEKLLDDLYAFSGQWLSKI
                     ESPYWNKIYAYEVQRVALSIVGNAYGSYPQAFMAPMQNDCGTDCSLYANPYPIPAEEV
                     ETKKPRENPCPEDSKLSLGFLACSADFDCESIEFGCSAGAAVSVKRNFKNKSTTLFVG
                     AGGEAHLGVAGGELKAGVTLTRTDGGDTDIGGKVEMSATVGGPVKVGKNMEMSATVME
                     GGKMETKNVIGVGL"
     gene            complement(34472..35557)
                     /locus_tag="PKI35_03030"
     CDS             complement(34472..35557)
                     /locus_tag="PKI35_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43385.1"
                     /translation="MKTIRTIIRLCSAVVIVSALNTANAQTALEYLNKMPKFPDSVCS
                     CSKEAMQQYAELVDDLKVKVTDDAENRKNQLESYIEGNKGSMENGMMQRVSETSGLSM
                     EEIQKLKDKQGDMTQAEKDALADRVLQNQNLSLDEVKNMQNMTKEGQEAWAQAYAAEQ
                     MANAQANPGQNAKMAQQNNQAASMLSLMEEQQSVQGKIRNYEMDVINRKQALLDEAVR
                     EKAALDSELKKLVNELHSIRSIYDCNECGTPPQADVNHWNAVVKKIREMQKSYRAYCE
                     KFTPKLEKYLNWYKESLVGNLPLYDKAEELQIQTTAATTNTNLSLPANAKGSFSLQAV
                     ENYMSAYDLFIFRSQIDLPDFQIDYYK"
     gene            complement(35581..36867)
                     /locus_tag="PKI35_03035"
     CDS             complement(35581..36867)
                     /locus_tag="PKI35_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX43386.1"
                     /translation="MKSIFRLLLLAVFMFSVTFASGQFNVGKKIENKVNNRINNKIDR
                     GIDKGLDGIEKSIKGDKKQENKTNNEAEEGKDNTGQDTKGDNKEGKAGLEAYSKYDFV
                     PGEKVIFFEDFSQDAVGDFPALWNTNGSAEVVTTNLYPGNWMKFVMNECVWTDALLKL
                     PENYTVEFDVIPIGGLENANMSGWNMRLMQARNAKAWDAGSAPGNGGIEFSIEYFGRP
                     SYHTWLYGQECEMAKLNGYKEGDAFKEKINQKYHIAIWVQKSRVRIYQNQDKLFDAPK
                     AFPTGCVKPDRLRFEYGAAMISNVRVAVGAPDMRNKLMTEGKLVTYGIYFDVNKDVVK
                     PESYGTLKEIAAILNEVPDVKVKIVGHTDSDGADASNLDLSKRRAASVKNELVKSFNV
                     NGDRLVTDGMGESQPVAPNDTPANKALNRRVEFIKM"
     gene            complement(36978..38276)
                     /locus_tag="PKI35_03040"
     CDS             complement(36978..38276)
                     /locus_tag="PKI35_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX43387.1"
                     /translation="MKALIRLMLLIALVLPVYVANAQFNIGERVQDKVNDRINNKIDE
                     GIDKGLDEVEGGLKGENKNSKKVEEDQDSENADENSSSEADENTQKPKSGKQDQPSLQ
                     SYSKYDFVPGDKVLYFEDFSQDAIGDFPALWTSNGSGEVKTVNIAPGKWFHLNGEDAA
                     YCYTKNIKFPENFIVEFDIIPDDEYRDGITFTLYEENPDDSKEMNDDLFPGNEGLHIN
                     LKSDGWETKGYRNEDNADMTGQSVKNPVLKEQVNHVIIWIQKRRVRIYHQGAKTLDVP
                     TNIFEGTVFNRMRFGGWDAKSWPMVSNIKITTASPDTRSKLITEGKLVTYGITFDVNK
                     AEIKSESYGTLNSIAEVLKANPDVRIKIVGHTDSDGADAANLDLSKRRAASVKNELVK
                     AFGIAASRLESDGKGESLPVAPNDTPLNKAMNRRVEFLKL"
     gene            complement(38696..39715)
                     /gene="fbp"
                     /locus_tag="PKI35_03045"
     CDS             complement(38696..39715)
                     /gene="fbp"
                     /locus_tag="PKI35_03045"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNX43388.1"
                     /translation="MKTLVEFINDKQIEYPGATGEFTRLLNDITIAAKIVNREIRIAG
                     LADINGMFGTTNIQGEQQKKLDVYANNQFINALKHGGQCAAIASEENEDLVIFGNSYS
                     QKARYVVAMDPIDGSSNIEVNVPIGTIFSIYRRISPEGTSATVDDLLQPGRKMVAAGY
                     IIYGSSTILVYTTGNGVNGFTYDAAVGLFFLSHPNMRIPDSGTIYSINEANYINFPDG
                     VKKYIKYCQEYDQATNRPYSTRYIGSLVSDVHRNMLRGGIFIYPGNLKNPHGKLRLLY
                     ECAPMAFIIEQAGGKASDGFTPIMDIEPYDIHQRTPIFIGSKSMVEKAESYMREYSGD
                     FSRAR"
     gene            39873..41156
                     /locus_tag="PKI35_03050"
     CDS             39873..41156
                     /locus_tag="PKI35_03050"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNX43389.1"
                     /translation="MKVFKFGGASVKDAAAVRNIASIIRNYATPQLVVVISAMGKTTN
                     ALEKVLQAWYSGSENLQQYVDEVIAYHQQIIGDLFSDSRHPVHFKTGLLFGQLEGHLC
                     TPPSLNFDFEYDQVVSLGELISTTIVSEFLVNEGFNCKWFDIREILFTDENWREANVD
                     WAKTGEKVKQQIRQFLINDSPEPRIALTQGFIGSTADGDTTTLGREGSDYTAAILTHL
                     LEADEMTVWKDVPGILNADPKFFDNTSLINRISYREAIELTYYGATVIHPKTIKPLQN
                     KGIPLRVRSFVSPASPGTIIGPASSGDDDQPSIILKSNQVILSFSPRDFSFIAEENLS
                     EIFSALASSSIRVNMMQNSAISFTICMDENERKMEKLTALLGEKFAYRYNNGLQLITI
                     RHYDQATINRVLAGRQLLVEQRSRQTAQYVVKTQD"
     gene            41366..42586
                     /locus_tag="PKI35_03055"
     CDS             41366..42586
                     /locus_tag="PKI35_03055"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNX43390.1"
                     /translation="MKGSVLILAGGGPAPGINTVISSVAKVFLRDGFRIIGLHDGYKN
                     LFTDNPSTIDIDFETADRVYNVGGSILRMSRYKPADLEFKTDFFKKNDVKLLVTVGGD
                     DTASTANRISKFLAEKHFPIQNIHVPKTIDNDLPLPEGQPTFGYQSAKQEGVRIGRTI
                     YEDARSSGNWFVVSAMGREAGHLAFGIGAACHYPMIVIPELFNHTNVTLDKITRLIVS
                     SIIKRKILGIPYGVAIVSEGVFHSMSDDEVEASGIQFTYDEHGHPELGTVSKAHIYNM
                     LLQIKLKQLGIDVKSRPVEMGYELRCIQPTAFDLLYCSLLGIGVKKLFDEGLTGCMVT
                     SDPTGDIAPLFLKDVEDKLGKVKTRMVNMESQKARMVFEDSIQYITEEDYPAASKYLK
                     NPEEYDFRKILKWE"
     gene            42843..45227
                     /locus_tag="PKI35_03060"
     CDS             42843..45227
                     /locus_tag="PKI35_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX43391.1"
                     /translation="MWNHLVRIIFRNRPAILAGIFIITALMAWQASDVKLSYDMLRML
                     PENDSSYVDYKEFKSRFGEDGNVLVIGIVNPDMFKLDEFNAWYDLGNEIKKIDGIQEV
                     ASITRAISLKKNDSIHKFEFLPVVARRASSQAEVDSLKEMIHGLKFYEGLMYNKSTNA
                     YLMAVTLDKKKLNDKSRTAVVTKIHEAAERYHNSTKHEIHYSGLPYIRTIQMEKIKNE
                     LYLFIVLSVVVAAFILFLFFRSFKIVLSSLVVVGISIVWALGLIGLFNFKITILLGAV
                     PSLIVVIAIENCIYIVNKYHWEYRLHRNKMKALSRVVQRIGYASLMVNTATAIGFAAF
                     IIVSNQMLRQFGIITSICVIMEYVLTITLLPIIFSFLKTPDEKNVKHLDNKFFGRILD
                     KIIYLIQFRRKWIYGFAGLLVLVGIVGITRIRTSGKVVDDLKKSDPISADLKFFEKNF
                     GGVMPFEISIDTKKKKGAMRMQTINKIDELQQEVLKHKEFSKPLSLAEAVKFAKQAYF
                     NGKPEYYTLPNSQERNFVLSYFPKKSEGKTNVLKSFLDSTQQITRVSFQMADVGTKEI
                     NRLLDSIKPQVESIFNKEDYTVSITGNSIVNARSTEFLIKHLFLSVGIAIALISLLMA
                     LMFSSVRMIVVSMLPNIIPLLITAAIMGFAGIPIKPSTIIVFSIALGISVDNAIQYLS
                     RYRHELKVTGGNIKLSTINALREAGFSMLYTSIVLVLGFSVFIVSEFGGTQALGILIS
                     TTLFIAMFFNIIVLPTLLLSLDKFIVTKAFEAKPIIEIYDGDDVNNGDLPVPEK"
     gene            45300..46169
                     /gene="rfbA"
                     /locus_tag="PKI35_03065"
     CDS             45300..46169
                     /gene="rfbA"
                     /locus_tag="PKI35_03065"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNX43392.1"
                     /translation="MKGIILAGGAGTRLHPITLAISKQLMPIYDKPMIYYPLSVLMMA
                     GVRDVLIISTPFDLPGFKRLLGDGSSLGCNFSYAEQKVPNGLAQAFVIGADFIGNDKA
                     SLVLGDNIFYGTGLAKLLQANNDPEGGVVFAYHVSDPERYGVVEFDQNNRAISIEEKP
                     KFPKSNFAVPGLYLYDNSVIEVARNIRPSARGEYEITDVNRYYLEQGNLKVATLSRGT
                     AWLDTGTFTSLLQASQFVQVIEERQGLKIGCIEEMAYRMGFIDRRQLEILAQPLLKSG
                     YGNYLLNLPVPER"
     gene            46176..47150
                     /locus_tag="PKI35_03070"
     CDS             46176..47150
                     /locus_tag="PKI35_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNX43393.1"
                     /translation="MHTLETIHQQISLAIEKLDIPGKPANLYEPVSYTLSLGGKRLRP
                     ALVMTACDMFGGSLDEAVHPAVGIELFHNFTLLHDDIMDQAPLRRGKETVYKKWNSNI
                     AILSGDTMFAMANREILKTSLSAVREIGDLFNLTAIQVCEGQQYDMDFESRDDVTLPE
                     YLEMIRLKTAVLLACSLKTGAIIAGASPGDCQLIYDAGISLGMAFQLKDDFLDTYGDE
                     KVFGKTPGGDILEGKKTFLFLKLIEKAGTEAKTFNRLYSPASALAAAEKIRFFTIKFG
                     EWGISEDVEREIDSFYKSALLSIDALSVDSVRKSVLLELIRKLSLRQK"
     gene            complement(47185..49131)
                     /locus_tag="PKI35_03075"
     CDS             complement(47185..49131)
                     /locus_tag="PKI35_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNX43394.1"
                     /translation="MISVSNLSVHFTGDYLFEDVSFLIGDRDRIGLVGKNGAGKTTLM
                     RIIAGELSPENGVVASPSGSTLGYLPQQIDSKSELCVLDEALTAFTEARSLHEKIEKL
                     TTALGERTDYESESYINLINRLHDATDRFHLIGGNLVREEAEKVLKGLGFDPEEFNRP
                     MNTLSGGWKMRVELAKILLRNPDVVLLDEPTNHLDIESIQWLEEFLISYRGAVMIVSH
                     DRAFLDNVTTRTIEIMSGRIYDFKAGYSEYVLLRSEQMELQKAAYDNQQQQIAQIEKF
                     VERFRYKSTKARQVQSRIKMLDKMDKIELDEVDTSAIHFKFPPAPRSGRVVVEAASLT
                     KRFGDKLVLNNLEFAIEQGDFVAFVGRNGEGKTTLAKIITGNLTHQGVCKLGHNVVIG
                     YYAQNQADLLDQEKTVFQTIDDIAVGDMRPKVRNILGSFLFGGEAVDKKVKVLSGGEK
                     ARLAIAQLLLKPVNLLVLDEPTNHLDMVSKDILKNALLRFDGTLIIVSHDRDFLQGLT
                     DKVFEFRNHTIKPYLGDVYDFLNARKLQSLKELEKMNAERQKEEDTITGNKVNYERKK
                     QQDRELRKAATTVKKLEDEIGQMEKEIRDWDQILNDPANHTDSAASAKLFKEYNRLKE
                     LLDEKVEKWSSAQDEYEKLQKENA"
BASE COUNT        12571 a        12214 c        11253 g        13296 t
ORIGIN      
        1 ccaactgagc ctgattcaaa gaaggaaacc gggataaccg gggcggcccg gccgggggtc
       61 agcgcttttc cctgtgcgaa acgaagatat attcgatcag gataacaaaa aggaccgtga
      121 acaacgaact aacgataacc cgcagcagcg tcctgaaaaa ttcggtaaaa gaaaagacat
      181 cgaggtaatt caaggcggca tgatgcagaa aaaccacgat agaagcatac gatacaaacc
      241 atttgagccc catatcgcgg atcgtcggaa attttgatga atccgattcg tcgtgtttgc
      301 caaccagtct gatgatggcg ggacgcgcaa atccggcaaa aaccgtggct gcggtatgca
      361 ggcccggagt gccggaaaaa aggtcaaccg tcagtcctgt gataaaactc agtgccagca
      421 gcagccagcc gggagtttca aaaggcagaa ggagaataaa gaggatgtag acaaaggggt
      481 tgagataccc gtaaaggttg attttattaa acagcagcac ctgcaacaac aggagcagga
      541 caaaccggat cacattctga aatacgactc tattcattgc cttgttcttc ggttgtttct
      601 ttaagcttat cctgttcctc cttcatcagg cttttcacga catagacgta atcgaggctg
      661 ttaaaatcga cgctgaaagc aaaaggtatc acataaaaat gttccccctg ttcaaccctg
      721 taatccttga tggtgccgag cataagccct gcagggaata tgttcgagaa accgctggtg
      781 acaatggtat cgccccgcga aagctttaca tgggccggta tatcggtcag cgtgccgatg
      841 gaataatcaa ctccgttcca aaccacggtg cccatctggt tgtttttctg aatgcgggcg
      901 ctgatgcggg tgtacttatt gagtacgctc atcacccagg caaagttttc cgatacgctc
      961 acgacggttc cggcaatgcc atccggggta atcaccgcca tatctttttc gatgccgaag
     1021 cgccgtcctt tgtcaagcat gatgtagttg ttgcgcttgt tggttgaatt atggatgacc
     1081 cgggcgacaa tatatttgta ttgctgcctg tacagcgtat cgtttttcca gaaggtattg
     1141 gtatcgtttt tcaggaacga cattccggag ccctgccgta ggatggaatt ctcgatggag
     1201 agaagttcgt tggtgcggcg gagcctgaaa tagcccgtaa cattgcttgc cgaagtataa
     1261 aacctgcccg tgatgttgtt gcctgcattc agcactgccg attgctggta ataggttgaa
     1321 ttgacgaaca tggtaatagc cactgcttcc agtacaacga aaagcagtaa aaaatggtac
     1381 cggactatga aggccaggat gtttcgcatg gatggctgcg gcggttacct ttcctgatgt
     1441 ttatttaata agaaaagtaa atttatcaaa gtttttaagg gcgattccgg ttccgcgggc
     1501 gacggcatgc aacgggtcat cggcaacatg gaccgggagt ttcgttttga gatggatcct
     1561 tttatcgagt ccgcgcagca gtgatcctcc gccggcaagg tagataccgg ttttgaagat
     1621 atcggcggcc agttcgggag gcgtcatttc aagggcgttg aggacagccg cttcgatttt
     1681 ggagattgat ttgtcgaggg catgggcgat ttcggcataa ttcacataaa tctccttagg
     1741 tatcccggtg agcatatcgc gaccgtgaac gggataatcg ggcggcgggt tatcaatctc
     1801 ggtaagggcg gcgccgactt ctatcttaat catttccgaa gagcgttcgc cgatgttgat
     1861 gttgtgctgt ttccgcatat attcctcaat gtcggcattg aaatcgtcgc cggcgatgcg
     1921 gatcgatttg ttgttgacga taccgcccag ggcgataacg gcaatttcgc tggtaccgcc
     1981 gcctatgtcg atgatcatgt tgccggtggg ttccagcacg tcgatgccga taccgatggc
     2041 ggcggccata ggttcatgga taaggcgtac ttctttggcg ccggcctgtt cggccgaatc
     2101 cttcacagcc cgttcttcca cctcggttat acccgaagga atgcaaataa ccattcttaa
     2161 agccggggga aagaggctgc ggttggtgcc gatcattttg atgaattcgc ggatcatgaa
     2221 ttcagccgat tgaaaatcgg cgataacgcc atcccgcagc ggccggatgg ttttgatatt
     2281 ttcatgcgtc ttgccgtgca tcatctgggc ctttttaccc acggcaatca ctttgccggt
     2341 agtgcgttcg atggcgatga tactcggttc gtcaacaact actttgtcat tatagataat
     2401 aatggaattg gccgtgccaa ggtccattgc aatttctttc gtgaggaagg aaaataagcc
     2461 cattgagatt atgtgatttc agtttcagta aagtattgcc agcttacacc tggctttaag
     2521 ttgtcggatt ttagtgttta aaatgccgta cgccggtaaa taccatggcc atatcatgct
     2581 cgtcgcaaaa cctgatgctg tccacatcac gaaccgaccc gccgggttcg atcactgcct
     2641 tgacgcctgc gttaaaggcg cattcaaccg aatcggcaaa cgggaaatat gcatccgaag
     2701 ccattacagc gccttccagg ctgaacccga attcgtgcgc tttgctgatg gcatgtttca
     2761 atgcgtctac ccgtgaagtc atcccgcaac cagagcctaa cagttgcctg ttccgtacaa
     2821 gcacaatggc gttcgatttc aggtgtttta cggcaatatt ggcaaataac aaatcgtcga
     2881 cctgtgaagc cgaaggggcg ttacgggtga ctactttcag catttcaggc gtttcggtaa
     2941 aacggtcttt atcctgtacc agaaccccgt tcagggccga ccggaactgt gttgcctgaa
     3001 aatcagcatt tttgcgctgc aaaagtattc tatttttctt tgatttcagg atttcaagcg
     3061 catttttttc ataccccggg gcaatgacta tttccatgaa cagtttgttc atctctccgg
     3121 cggttgtttc gtcaatcacc cggttggttg ccacaacgcc cccgtaagcc gaaacagggt
     3181 cggcggcgag cgcgtcagtc caggcctgta acaacactgg ccgggtagca agcccgcagg
     3241 cgttggtatg cttgatgacg gcaaaggcag gcccggcaaa ttcatcaatc agtgagatgg
     3301 ctgattcaag gtccacgagg ttgttatacg aaatctcctt gccgtggagc tgctcaaaga
     3361 catcatcaag gttgccgaaa aagattccct tctgatgcgg gttttcaccg taacgcagcg
     3421 tcctggccgt ctgactgctc accttaaagg cctgaattcc ggcatcccgg ttgaagtagt
     3481 tgaagatagc gctgtcgtaa tgcgaactga tatcaaaagc ccgggcggca aacctgtaac
     3541 ggtcggccag cgatgtaagg cctttctttt cgttcagcag gcttattaat tcgttatact
     3601 gatccgttga ggacacgata accacatcgt tataattctt ggctgcagcc cgtatcagcg
     3661 aaatgcctcc gatatcgatc ttttcaacaa tgtcgttatg gcttgccccc gaggcaaccg
     3721 tggattcgaa aggatacagg tcaacgatta caaggtcaat gggcggaatc ccgtaattct
     3781 ctcgttcggc aacgtcaccg ggattctcac ggcgaaaaag aattccgcca aacaccgccg
     3841 ggtgaagtgt ttttaccctt ccgccgaaaa tcgaaggata cgtggtaagg ctttcaaccg
     3901 tggtgcaggg caatcccagc gaacggataa aatcatacgt accgcctgtt gaatacatca
     3961 ccacgccaag gctgttcaat aagcgggcaa tggcatcgat gccttccttg tgataaaccg
     4021 aaatcaacgc tgacctgatg ggttttagct cttccataat ggctaccata taaaggatgc
     4081 aagtatatta aaaaatgtgg ctggtaaggt ccgtttcgca aaataatgaa tacatcccgg
     4141 ggcagtaatt acgaaaagat gtaattttac gctatggggc caggagcagg attggccctg
     4201 aaccggattt ttattccact tctcaaccca ttattgatgc tgtttctccg tttattccgc
     4261 gaaagctatc acctggcgct acaggccatc agcgcgaata aattccgcac tttcctgtcg
     4321 ctcatgggaa tcactatcgg catattctcc gtaatttccg tgcttaccgt cttcgacagc
     4381 atcgaaatca cgatccgcaa aaacattgaa tcgcttggca gcaatgtgct gttcatccag
     4441 aaatggccct ggagcatggg cggcgacgaa tatccctggt ggaaatacat gaaccgcccg
     4501 gaagcccgcg tccaggaaat ggatgagatt ctgcggcgca gctcgcttac cgacgcagcc
     4561 ggctattatg ttgctacaaa taaaaacgta aaatacgaag gaaatacttt tgaaaacgcc
     4621 acgattgtag ccgtatcgca tgattacgac aaagtgcagg cctttaacat tgacgaagga
     4681 cggtatttta cccccctcga atcgcaactg ggccggcctg ttgccattgt cggaaataag
     4741 attaccgctg aaatcataaa ggatgccgat ccaatcggga aacgtatcct gattttcggg
     4801 caaaaagttg aaattatcgg gacttttaaa aaagaaggca acgatacttt cgggcaatca
     4861 aacgatgaat gggttatgtt acctgtcaat ttcgggagga atttcctgga cctgaacagt
     4921 gataatatcg aatcggccat cctggtcaga agcaaacccg gcatccctaa cgatgcatta
     4981 catgatgaac tgacgggcat tatgcgttcc gtaaggaagc taaagccatc ggctgaagat
     5041 aatttcgcca tcaacgaaat gagcatcatc acccagggat tcgacaaaat tttcagggtc
     5101 atagcgcttg tcggatggat tgtaggcggt ttttcgttgc tggtgggcgg tttcgggatt
     5161 gccaacatca tgtttgtttc ggtgaaagag agaaccgccc agatcggcat ccagaaatcc
     5221 ctcggggcaa agaactattt catcctgctg caatttcttt tcgaagccgt tttccttgct
     5281 ctgatgggcg ggattttagg tttgctgatt atttaccttg gaaccgttat tgtttcgaat
     5341 gcagccaaca tgacgctggt actcacatca ggcaacatta tcctcggcct gtcggtttcg
     5401 gcatttatcg ggctggtttc aggtttgttt ccggcttggg cggcctcgcg tctcgacccg
     5461 gttgaagcca tgcggtcaac gttctaaagg caggatcctt catcctggtt tttcacatgc
     5521 tgccggtccg gctgaccggt attttgattt gctcatcatc attgaaattt caatgttttt
     5581 ataagagcgg tgtccatatt tcctttttta taactttgtg agattacaat accttatgga
     5641 aaaacgctgg gtttttaaag agaacggaaa tacagagttg gtcagggacc tggctgcaag
     5701 tttatgcatt gacgaaccgt tagccgggct gctggttcaa aggggcgtca catcctttga
     5761 agaagcccgt aatttcttcc gccctgattt gcagaacctg tacgatcctt ttcttcttaa
     5821 agatatggac gttgccatca gccggattga aaaagcccgc gatgccggcg aaaaaatcct
     5881 ggtttacggg gattatgatg tggacggcac atcggccgta gcattggtat atactttttt
     5941 caggaagcag ggattcgaaa atcttgattt ctatatccca aaccgttaca gcgaaggata
     6001 cggcatctct ttccagagca tcgattttgc cgctgacaat ggtttcgctc tcgtgatagc
     6061 ccttgattgc ggaattaaag ctgttgaaaa agtaaaatac gcccgtgaca aaggcattga
     6121 tttcatcatc tgcgaccacc accggcccgg cgatgaactg ccccctgctg tggctgtcct
     6181 cgacgccaaa cgcgaggatt cggtttatcc tttcaatgaa ctttcgggct gcggggttgg
     6241 ctttaaactc atccaggcat acgccaaaaa aacaggcatc gctttcgata acctgtacga
     6301 ataccttgac ctggtagccg tcagcatcgc ctccgatatt gttcctatca ccggtgaaaa
     6361 ccgcatcctg gcttacttcg gattaaaact tatcaactcg aagccccgcc ccgggattga
     6421 agccatcctg aacgttgcca atatcattcg ccgggaagag accgaaaacg ggccggtaac
     6481 cgaaacgggc attttcacca gggaattgac gatcaacgac gtggtattcg tcatcggccc
     6541 ccggatcaac gctgccggca ggattgaaag cgggcggaat tccgtgcaac tgctcataag
     6601 ccaaagcctt gccgacgccg aaagcatcgc ccggcagatc aacgatttca acatcacccg
     6661 ccgcgacctt gactcccaga tcacccagga agcgctcgat atgatagctg ccgataaaaa
     6721 tttacaggga ttacgttcaa cggtcgtctt caacgaaagc tggcacaaag gggtaatcgg
     6781 aatcgtagcc tcgcgtctca cggaattata ctaccgcccg actgtggtgc tcacccagtc
     6841 aaacggtttg atcaccggtt cggcccgatc ggtcaaggat ttcgacatct acgatgccat
     6901 cgatgcgtgc agcgacctgc tggaacattt cggcggacac acctatgccg ccggcctttc
     6961 actgaaaccc gaaaatctta aacctttcaa ggaaagattc gaacaaattg tagcctcctc
     7021 catcgaagac cggatgcttg tgcccgaagt tgaaatcgac ctgatcctcg acctccacca
     7081 gatcaccccg aaatttctgc gcatattaaa acaatttgcc cctttcggcc ccggaaatat
     7141 gtcgccggta tttatgaccc cgcgtacgcg tgatacaggc tatgcgcgca tcgtaggcaa
     7201 aaaccatgta aaaatgacgg ttttccagga atcggtccgc agcgatccat tctcctccat
     7261 agcctttcaa cagggggata aatataacag gatacagaaa ggattgccga tggacatctg
     7321 ttaccatatt gaagaaaacg agtggaacgg caacaaatcc atccagctca atattaaaga
     7381 tatacggttc tcggcagaat aataaccgct cccatcgcac ggaatattta cagccggacg
     7441 gtttttttca cccaaagagc cgaaagacgc caccgttcca ttccccgttg catggccgct
     7501 cccttaatca accctgagca ccaacccctt cagatattct ccttcaggtt gaaaaatatt
     7561 tatcggatga tcgcccgact gccctaactg ttccagcacc cggacgctcc gccccgactc
     7621 tatggcagca gcggctactg ccgcgttgaa ttgttcccgg ctcacaacct gtgaacatga
     7681 aaaggtaaat aagataccgc cggctgcgat tttcttcata gctgacgcat tcagcgaggt
     7741 gtatcccctg atggcgttgt ggcgcgacga agtatttttg gcgaatgccg gcgggtcaag
     7801 aatgatcata tcatactccc cctgtttcat ggtttcaagg tactgcctgg catccgtggc
     7861 aaagactaag tggcggtcgc cgccaaaacc attgatggca gcattttttt cggccaattt
     7921 acatgccggc gccgaactat cgactgaatg aacaagttcc gcgcccgatt tgagggcata
     7981 taccgaaaag cccccggtat agctgaacat attcagcacc tttcggcccc tggcatataa
     8041 gccggctttc ttcctgtttt cacgctggtc gaggaagaaa ccggtcttct ggcctccttc
     8101 aatatcaaca acaaacttac aaccgtgttc cgcggcgata acatcgctgc taccgccggt
     8161 gagccagcca ttttgcaccc tcagaccggt atttttaaag agtgattccg aacttttatc
     8221 atagatgccg gagagtttcc cgccataaat ctgcatgacg gcttccgcaa gatcctgccg
     8281 cgcctggtac attccggcag aatgcgcctg aagaaccgca accgaatcat aaacatcaac
     8341 gattaaaccc ggcataccgt cgccttcgtt atgaaccagc cgataacaat tcgtatcagg
     8401 atttgaaacc aggttcagcc cttcacgaag ggaataagct tttctgacct gtttgtacca
     8461 gaattgccgg tcaatcggct gcctttcgaa agtcaggatt tttacggcaa tggaactatc
     8521 ctgaaaatgg cctgaagcaa ggtatttccc attggaacca agcacttcga cgatggcgcc
     8581 atcttgcagg ttttcactaa cagacgctat ggctcccgaa aagatccagg gatgaaaacg
     8641 caataccggc acctctttgc cgggtttcag gataactctg ggataatcca tcgcaacaaa
     8701 atttgtcaca aaattaatta ttggccttat atttgcatta aaagcgatta aattcattgt
     8761 ccgcattttg aaattcaaca tgttaggcaa taaacaaaca aaaacctgca attaatcgta
     8821 tatttgctcg ttattcattc atcctaaatc aattcaccca atgaagagaa cccttttcct
     8881 tcttattatt ccggcgctat tactaaccac cagttgcaac aagcaaaaag aggaaattgc
     8941 ccggctcaaa gcaaaaaacg acagccttct cacgctcaaa ttcacgagtg ataccactgt
     9001 gattgaattt gtgaaagcct tcaacgacat tcaggctaac cttgattcga tcaaacagaa
     9061 agaaatgatc ataacgaaaa gcaccgaagg cactactgag ttgaaacaga atgccaagga
     9121 ccagatcagc agcgacatca attcaatcta tctgttgctg aaaaagaaca aggagatggt
     9181 tgccgcattg cgcgccaaac tgagcaaatc ggacaagcgc attgttgagc tcgaaaccat
     9241 gattgataac ctgaacaaac agattgaatc gaaagacctg gaaattgccg acctgaaaga
     9301 atcgctcagc aaacttgata tcaaggttaa agatctgagt tcacaggtag acaacctgaa
     9361 tgcaaccgtt gataacctga gttccgataa caaggccaag cagcaggcca tcgacgaaaa
     9421 gaccactgaa ctgaacacgg catattatgt gatcgggaca agttccgaac tgaaggccaa
     9481 gaatatcatc acccgggaag gcggatttat cggcattggc cgtaccagta acctgaagaa
     9541 cctcgtaaac atggacaact ttacgaagat cgacatcacc aaggttcatg aaattcccat
     9601 catgaaatcg aaggttaccc tcatcacaac ccatccgtca aattcgtaca aactggaagg
     9661 ggaaaaacct gttgaaaagc tggtcatcac cgacccgaag gctttctgga gcatttcgaa
     9721 atacctggtc atcagcgcca agtaacatcc tcctgatata ataaaaaacc ggctcatctg
     9781 gccggttttt tttatttcag tggatttctt atctggtata aatcactgac cgatatccca
     9841 aatttaaaga aaagcgaggt atcttaagta ccggtcaatg aatggatcct tcgggcttta
     9901 ttcgattaac accttttctt tgttgtaatc aacttccctg atcactttgc cgttggcatc
     9961 ataaaagcgc cagattccaa ctttattgtc ggcgctataa ttgccttcga aatacagttt
    10021 tccgttctca taatatactt ttcccattcc gtcgcgtttg ccatccttga aatccccttc
    10081 actccacagg ttaccgtttt cgtaccatgc tttccacacg ccgctgcgct tcatcttggt
    10141 aaactcgcct tccatttttt tgtttttgtt ggcgtaaaat tgctgttcac gcacaacctg
    10201 tctttctcca ttcacttttt cgtaatattt caccactttc ggcgaaccgt cttcgtattt
    10261 ttcttcaatc acttttttag ggccgcaggc aaaggccagg cttgctaaaa ggatcagcac
    10321 caatgttttg taaagtctca tttcctgtta aatgttaatc gtttaccggt aaacgaatga
    10381 tcgaatttac cgttttcata aattaaattt ccgtttacaa aggtatgggt tacctgcgaa
    10441 ttaaacgtaa ttccttcgaa aaccgaccag ccgcatttgt aaagtatatt accgggatcc
    10501 acggtccacg gggactgagg atcaacaata acaaggtcgg caaaataacc ggggcgcaga
    10561 tatccgcgtc tttctatgtt gtagcagtcg gcaggggcat ggcacatttt ttccactact
    10621 ttttccagtg taatcttccc caacctgcac atttcgagca tagccggaag ggagtgctgc
    10681 accagcggcg ttcccgacgg ggctttaaaa taagactgtt ttttttcgtc cggtaaatgg
    10741 ggcgcatgat cggtggtcac aatatccagc ttgttattga gcaaccccct gagtaattct
    10801 tcccggtcgg aagccgtttt tatggcggga tttaccttga tcttcgttcc gagcctctga
    10861 taatcttcgt cgcaaaacca caggtaatgc acacatgcct cggctgttat gcgtttctgc
    10921 gaaagaggga gcccattttc cagcaaccgc atctccgcgg cagtggatac atgggcaata
    10981 tgtaaccgcg tattgaactt ttgtgccagc ttcacggcct gttccgacga cttgtaacat
    11041 gcttccgaac tcctgatcag gggatgcata cttaccggca cctcttcccc gaatttttct
    11101 ttcatcaggg cgctgttata ccggatcatc gcttcatctt cgcaatgggc aacaacgggc
    11161 accggcgaac gttcaaaaat ttcctccagc acatgcgcgt tatcaaccag catattgccg
    11221 gtcgaagagc caaaaaacag cttgactccg ccgacaatcc cgggattggc attttcaatt
    11281 tcgcggatgt tgtcattggt cgcacccagg aaaaatgaat aattgacaag cgattttgcc
    11341 gaagccagcg aacacttttc ttccagcaaa tcaagtgtgg ttgtctgagg cactgtattg
    11401 ggcatttcca tgaaggaagt aacgccgccg gcagccgcag cgcggctttc ggtaaagatg
    11461 tcacctttcc gggttgcgcc cggttcacga aaatgaacat gacagtcgat aacgccggga
    11521 atcagcaact ttcccccggc atcaaaaatt ttgccggtta caggttcctt taaaccgctt
    11581 tcgccaattt gcgcaattac cccgtcccga atggttatcc atccgttgta tttacgtccc
    11641 tcattgacaa tgaacgcatt gcgtatagtg aatacatttt ccatagactt gtttcaggtc
    11701 ttgattacaa agatattcaa aagagtggcg tatctttgca cgagtgttga atgaatgata
    11761 tccggcgcat gtactctcgt aaactgttct tactctcaat ctcctttctc attgcttttc
    11821 aggtcaacgc tcaaataggc ggactttccg tatcgaagtt aaactccttt tgtgcggatg
    11881 ccgttgaccg tcaccaactt attgttgaac ccggtttttc gtatactacc agcaaaaaac
    11941 actggaataa caacagtgat cttttacgga tatactccac caacgacagc ctgaatgctg
    12001 aagccgggct ccgttttcgc ttcacttacg gcgtttccga taatcttgaa atcggtgtta
    12061 cggtccccgc cgacatgtct acgagtagct ggggcgttaa atatctttta ttccaggatg
    12121 aaaaatacgg gtttgctgct attggcgggg tgaattatcc tttcggaaaa ggcgccatca
    12181 ataaaaaaga cgctgaaaac gctatacagg gcggcttggg cgccgttttc acctggtcgc
    12241 cgacggaaac tttctcagcc gatgttacgc tgcaagccgc aaaatacata ccggaacctg
    12301 aatactcatc cgaaggatat ttttcgttgt cggccgatgc cgggtattac ctgtttaacg
    12361 gcaacctgct ggttgcagcc ggactcgggt accaaaaata cttctctgat aacctgaaac
    12421 agtattcctt taccctgtac cccggtattg ccatcaacac gggtaagaat ttctgtataa
    12481 cccttatcca ctcttttgac ctggcaggca ggaataccga taaacagcaa attacggatg
    12541 tttcgtttac cttaaccctg gattaaactg taaataccgg attataaaca tcgcaacccg
    12601 cgctcatccc gatcttatga gccggtcatg attttacact gccagatatc cttttactca
    12661 tcctgatcat aatgccgctg ataagccctg aaagccacgg actttccggt ttatttgcta
    12721 ctcccggccg ccaggcattt atccggcggc ccgagtgaat acaaggattc atcaggaatt
    12781 ataaaaaaaa ctccctgtac aggacaagga gtaaaatgtt tgcgtctctg atgcttacat
    12841 gaaagcaggg aagaacccgc cggaactttc ccggcaattc tttctgcctg tttaggcagc
    12901 cctgggccct atctttttaa accgcaggct tatcaccccg aaaaaggctt ctttaaagat
    12961 ttttgaactc atcttggagg ttccttcggt acgatcggtg aatatgatcg gtacctccac
    13021 caggttgaac ccaagactcc atgcggtata tttcatttca atctgaaaag cataacccat
    13081 gaatttgaca gcatccagat caatggtttc cagtactttg cgggtatagc atttgaaacc
    13141 ggcggtagta tcgtaaatgg gcatcctggt taccagccgg acatattttg aagcgaaata
    13201 cgacatcaga acccttccca ttggccaatt gaccacgttg acgcctttca cgtaccgcga
    13261 accgatcgac atatcagcgc catacgaagc gcaggcgtca taaagacgga ggaggtcgtc
    13321 ggggttatgc gaaaagtcgc agtccatctc aaaaatgtaa ttgtaatcgc gttcgagcgc
    13381 ccatttaaaa ccggcgatat aggctgttcc aagtcccatt ttgccttttc tttcaagaat
    13441 gaaaagccgg ccttcatatt caggcatcag gctgtgaaca atcttaccgg taccatcggg
    13501 tgaattatcc tcaactatga gcagatcaaa cggtttagac aacgaaaaaa ccttacggat
    13561 catcttggcg atgttttctt tttcgttgta agtaggaatg attacgagac tgtcggacac
    13621 gatcggttat ttttggtaac gaaagtatgg atttatttca aaaagagaat cgttattagt
    13681 caattatttg aagccggcat caattgtttc ttatcatctt cagcatacat aagcctttgg
    13741 tggtatttac cctcacgagg taaacgcctg ccggaagttc acttaccgga agcacggccg
    13801 aggattgcct caccataccc ccggcggaga acctgcacac ccctgacatg tcataaacgc
    13861 tgatgctttc aatataggct gaagcatctt tcatatcgat gttgacataa tcggaagccg
    13921 gatttggata acaaacgaga gatgaagcgc cggcggcgat ttcttgcaca gccagaattc
    13981 cgccatttct catgaaccgt gaagcaaact cctggaaata gaggttcacc aggccggcat
    14041 catggattac cagcgtgttt tcatcgttgc gggtatcggc gcttgtgctc cagttgtgtg
    14101 agccggtaag cacctgcggg tccgatgatg cgtctttccg gtcgataaga acatatttgt
    14161 tatgttcaag cccggaagta tatttatcgg aagtgaagtg cgttcccaat ccgtgtaaaa
    14221 catttgcaac agcgggatag tttgtcagat cgctctcgct gtcaataatc atattcactt
    14281 caaccccggc gttcacccga tcctgtattg cattggcgat atcggtgcgg gttatgataa
    14341 aaacggcggc atcaacattg gaggaagcag aaccgatgct ttcgataata cggtcgttaa
    14401 cgccatccga agggctgaaa tagctctcaa cgtcttttcc cccgataata aactggtgag
    14461 gagtgttgtc ttttttataa ggcccgaaac gggcattggc tgctgaaggc gtcatatcct
    14521 gcgaacccca catttcttca aattcaaggg tgtacgcttt tgccagactc tggtcgttga
    14581 tgatgataac attgttggcg tcgctgctaa gctgctggcc ggtcatgttg gtggaaccgg
    14641 tccaaacaat ggcatccgct ggatttggag aaccggaatc aaaaacaaaa aatttgttgt
    14701 gcatgattcc gtaatcactc ccgtcgggac tggccagttt gggaatggaa ggcacccact
    14761 gcatcgaggt attggaagta ttgctgttgt aaacaatcct tactttgacg cctcgctgta
    14821 cagcctgatt cagggcatac ggaatacggt cgagggtgaa attgtacatg gccatatcaa
    14881 tggtatagcc ggcccggttg atgtatgcaa tgagtgtatc ctccactttt gtccccagcg
    14941 agatggctga cgggcccgaa gcaaacgaaa catccacgtt gctggtaaaa taggctttca
    15001 tccagcccgg cgataaagat tgggtaataa acaccctgac cggtgcaaag gcggtatcgg
    15061 taccgcttgc gctgaaagcc ttcacataaa acagttcgga tgcctgcgct ccggtgatat
    15121 caaccgaatg gtaagtcgta tttccggcgg aggacaattg gccgagttca agctgagggg
    15181 tattgccata aaaaattccg gaacttgcag ccacatcggt aatccatgaa accgttaagc
    15241 ctgatgtaga aatgttttcg gcacggggca aatccgtaat gtttacgctg ataattccgc
    15301 cggtgatgtc ggattgcccg cgcaacagca actggtaatc gctttggtac tgggaacaga
    15361 tgcccgtcag gctgatctca ttgtatggaa tggaggtgcc gataagcggg tggcccggac
    15421 gaacatagac cgcgccttgc tgccctcccg atgtgaaagt atatgtcccc gcgtcgaagg
    15481 agcttccctg tccggcatcg aaggcaacgt tgctgatctg cacgagttga gactcattgc
    15541 tttctgaaag ttgtgacggg gtgatttgca ggggaacggg aagcgggttt ccggatgagt
    15601 tgatggtgaa agaactgaca gggcttatct ccagcaggtt ataataattt ttcagggtac
    15661 cggtcaccgt aatttgctga ccggcctgaa cactccccag cgaatttccg taaactgcaa
    15721 tgcccgccgt ggaatcctgg atataacgaa tggcgcccag ttcatccccg ttgattacaa
    15781 taccgctgac ggtcactgtt gttccggacg agagcaggcg ggcttcagca atggtcgttt
    15841 gtccgtaaac agccagtgaa aaaaacatca gaactataga cgcgagtcgt ttcattgttc
    15901 ggaatccctt gcaggatcat tcttgagatg ttaattttat tttcaggcaa gtatgccgta
    15961 aaaatgagac gaatcaatgg acttctttga caatcactgt gtaaaccggc aggtgatcgc
    16021 tgtatcctcc ttcgtaggcg ccaccggcat aggtgcggaa aggataacct gcatactgtc
    16081 cttgctgaga tatcaggtat ggtttgttga atacttttgt tttcagccag taccaggacc
    16141 cggatttttt atcaagcaac ggggctgaaa ggatggtttg gtcgaacagg ttccaggaat
    16201 cgcggtaagc cagggaacca atcccctctt tatacatctt ccacatggga ttaaaaagca
    16261 ttttctgctt cactttctgt tcacggcctt cggcgcccag gatcacattt acgctttcat
    16321 ctaccggatc gtcgttcagg tcgcccatga tcactatttt tgcatttctg ctcaccctga
    16381 ataatgaatc ggcgaggctt cgcgtcagcc gggcagcttc agccctgaat ggcggcagat
    16441 tcccgcgcga aggccagtgg ttcacaatca cgctgatggt atcgccttca agcaaccctg
    16501 taactacaag ctggtcgcgc gacctccagt gctcatgacc gggcatcagc aaccttgccg
    16561 acaccgtgtt gataacggtg aaatcatgtt tccggtacag caggcctacg tcaacgccac
    16621 ggtaatcggg tgaatcataa tgaacaattc catacccgga attagccagg gccggcatat
    16681 taataagatc ttccagcact ttgcgatttt caatttcgga cacgccgatc aatgtaggac
    16741 cgcctttgat atactcttcc ccaatctgag aaatcacctt cgacatgttg gcgagttttt
    16801 tctggtaacg ttcgtccgtc cacttatttg cgccttccgg caggaattct tcgtcgttga
    16861 tgagggtgtc attaacggta tcaaagaggt tttcgagatt gtaaaaagca atggtggcaa
    16921 cttttgcttt ctgttgttgc gctaatgacg gaatgtgcgc caggagcatg aaagccccgg
    16981 ccgcgaacag gaacaggagt tttttattag tcatcatgga tttggaattc actggcaaaa
    17041 ttaccgtttt attcatcacg atgaacatca ggggaaattg ctggaattga gaccacatta
    17101 accaataatt tacttttttt ttgttcattt gtactcccgt aaattagccg ctcaaaatta
    17161 aagtcattca gtatttacag ctaaaatcta cacatgaagt tcttcaacgc actagtcgta
    17221 tttttccttc tttctgcaac ggcatttgcc caatcgtata gcatttccgg cgttgttaag
    17281 gaaggactta cggacaagcc gatcatccat gccacagtcg gggccggatc tgtgaccggt
    17341 tataccaatg acgacggttc cttcaggctg cacggaatcg ccggaggaaa atatacgctc
    17401 atcgttacca tggcgggtta tgaaacgctt tcggtaccgg taacagtggc cgatgggcct
    17461 gccgatgtag gcaaattaca aatgcagcca tcgcgggctc agggagaatc gggcgttgcc
    17521 gagatcacta tcggcgacga tggtgatgag aataacgagg ctgtttcggg cctgctgcat
    17581 tcatccgacg atatatttgt aaacacatct tcgtacactt tcagttccat gtttttcagg
    17641 ccccgcggct atgattcgga atatgaaacc gtgcttatcg acggcattcc gttcaacgat
    17701 gccgaaaccg gacgcgccgt atggggcgaa tggggagggc tcaacgacgt gatgcgcaac
    17761 cgtgaataca tcaacggatt gcagccgggc aacttttcgc tgggcaatct gggcggttcc
    17821 tcctatatca ccacacgggc ttcgaaacag cgcaagcaaa ccaagctttc ctattcactc
    17881 tcaaaccgtt cctacaccaa ccgcatcatg tttacctatt caaccggcat gatgaagaac
    17941 gactgggccg ttacggtgag cggttcacgg cgctggagcg aagaaggcta tgttgaaggc
    18001 accttttatg acgcttacgc atgcttcctg ggcgtggaga aaaaaatcaa ttccaggcac
    18061 agcctttcat tttccggctt tgcttcccct acccgccgcg ggatgaactc cgcctcggtg
    18121 caggaagttt acgaccttgt cgggtcaaac tattacaatg ccaactgggg ttaccagaac
    18181 ggagataagc ggaatgcccg gatacggacc gtcaatgaac cgaagctcat cctgagcgaa
    18241 tactggacca ttgacgaaaa aactacctta accacttccc tgtcgtacag tttcgggaaa
    18301 acagggacta ccggcctgaa ctggtacaat tcgagagacc cgcggcccga ttactaccgc
    18361 aacctcccga attatatgca agatccgacc gatacgctta attctgttgt gcaggctgcc
    18421 attattgacg cctggaagaa cgacccttcc gtaagccaga ttgactggaa ccggctttac
    18481 caaaccaatt acctggccaa cctcgaagga aaacaggcgc gctatatcct tgaaaacaac
    18541 ataaccgccc agaaccagtg gtatctcact tcgcgcatca acaggcaaac cggagagcac
    18601 agcaacttca gcggcggcat tgaactcaac cattacaaag gttctcacta taaaattctg
    18661 gaggacctgc tgggcggaaa atactgggtg gatatcgacc agttttcgga acgggatttc
    18721 aaaaccgaca aggaaaaatt gcagaacgac ctgaacaatc cgaacagggt gatccgggaa
    18781 ggcgaccgtt tcggttacaa ctacgacctc ctgagcaata ctgcaaacct gtggggactc
    18841 ctggaactca attatcctca tttcgattac tatatcggcg cggctttcgg tggtacccaa
    18901 tactggcgtg agggctttat gcgcaacgga aggaatcccg ataactctta cggcgtatcg
    18961 gagaaacaat caacgctgac gtacgacatt aaggcaggct cgacctggaa ggttaccggc
    19021 cgtcattttg taacggccaa tgcagcgtat atcatgcgtc cgccgttgct gcgcgacgcg
    19081 tatatttcgc ctcgtaccgg cgccgacctg acccccggca ttaccgacga aaaaatactt
    19141 tcgggcgata tttcctatgt agcccgcttt ccaaacttca acgcacgggt caccctttat
    19201 gagaccttct ttaaagatta ctccaaaata ctccgtttct acgccgacga tttcgaaact
    19261 tacgtaaaca tggccctgac cggtgttgac cgcattcacc aaggtattga attcggggcc
    19321 gaaaccaaaa tcatcgacaa cctgaaactg gtcaccgttg cttccttcgg caactacagg
    19381 tacaccaacc ggcctaaggc taccatcaat tacgataatg gttcggaacc tgacactacc
    19441 gaaaccgttt acgccaaata cttctatctt ggcggcacgc cccagtttgc cggaagcgtg
    19501 ggcctgaaat acaataaaaa ttactggttc tttgacctgg acgttaacta ttacgaccgg
    19561 atatggcttg attttaaccc catgcgccgc acccaggccg ccatcgccaa cctgggagaa
    19621 ggcgatcctc tcatcgaact tatcacggaa caggagcaac ttgacggagg ttttaccgtg
    19681 gatgcatcca tcgggaaatc catccgcatt aattataagt acttcgtcaa catcaacttc
    19741 tcagtcaaca acatcctgga taataccagc ctgatctcgg gaggatatga gcagaaccgt
    19801 tttgattaca cccatgaata tctgcagaac ggcattgata agtttcccgg caagtactat
    19861 tattcatacg gccgtacctt cttcctgaac gtagggttca ggttctgata tgccggataa
    19921 taaacacctc ataacattca ttacgcataa aacacccaaa tgatcatgaa aaaatatatc
    19981 agccgcactt tactcttcgg cttcctggcc ggaatactga caattacagc ctgtgttaaa
    20041 ggcgatttcg acgagccgcc catttatatc ccgcatgttg attttgagtc aaataccagc
    20101 attgaaacac tgaaaacaat gctcaatggt acgctgacaa ccattaccga tgatatcatt
    20161 atcaagggaa aagtggttgc caatgatgaa tcgggcaacc tctacaagaa aatcgtgatc
    20221 caggacgaaa cagcaggtat tgaagtattg cttaatgcag taaacctgta taatgagtac
    20281 aaacccgggc aggaagttta cattaagtgc aagggtatgt acctcggttc ctataaagga
    20341 ctcattcagc ttggctatga ttataacggc ggaataggcc agttgccgga ggtaatgatc
    20401 aatgagcacc tgtaccgcga cagcctgccg ggaagtgtgc ccgaaccggt ccagatcact
    20461 ccgggtgata atgttaataa tgccaaatat ctgagcatgc tggtatccgt cgacaatgcc
    20521 aatttccccg aaggtggaac aaccgtctgg gctcctggag atgcatcaac cagccactcg
    20581 ctatttgttt ccggaaagga actgattgtc cgtaccagca attacgccaa cttcgccctg
    20641 gataccattc cggccggtta tggcaaagta attggaatct taagtatcta taatactgat
    20701 tatcagttaa ccttacgtga tgcagccgac ttgcagggct tctcgggtga aacaccaata
    20761 tccgccgact ttgtattccc tgccacggga ctatccccgg taaccagtat tgatgaaaaa
    20821 ttcgacaata ttgttgcaag cactgacgtg gctatcaacg gatggaccaa caaggcggca
    20881 gccggttcac gcaactggca gggtaaaact ttccaggctg aaaaatatgc ccaggccacc
    20941 tcttataata caaccgacgc tgaaaataaa gcgtggctga taacaccacc ggttgtctat
    21001 tcttcctccc tgaagctttc gttcagctcc gaaaaagcct attataaaca cgacggctta
    21061 agtgtgtgga ttctcaccaa ttataccggt gacccgaata ccgcaacatg gactaaaatt
    21121 aatgcaacgg tggctggttc tgccaatgct gattacgaat gggttccctc gggcgatatc
    21181 aatttgtcgg attatgtgcc ggccaattat acgggcaatg tttatatcgg attcgaatac
    21241 acaggcaatt caacaaatac aacgacctac tgcatcgata acatactggt gggaaccggc
    21301 ggtggcagcg gtggcggaac attgctggat gaaggtttca cgactgcgct ggggtcattt
    21361 tctgccgtca gcgttaccgg atcgcaggcc tggggctggg ccgattatga cgaaggatcg
    21421 gccaagatga gcggttatgc aagcggcagc aatcatgcca atgaggattg gcttatctca
    21481 ccgcagattt cacttgccgg taaaacaggc gtaaaaatga gcttccgtga agccatcaat
    21541 tatattacgt ccattaatga cctgaaagtt ctcgtttcga ccaattacag cggatcaggg
    21601 gatcccaacg cggcaacatg gaatgaagtt acaggcttta cacgcgctgc cggcaacaac
    21661 tggacttttg ttgacgcagg ccaggcgagc ctctccgctt atgaaggaca aaatattcac
    21721 attggcttta aatatgtgag cagcgcttca gcaggcgcca cctgggaaat tggccaggta
    21781 gttgtgacct cctccaaata aaagcgaata taaaatgaaa ggccggtgaa caaccggtct
    21841 ttttttttgt accaatcgtt gctgaattac tgagccggca gaattttatc ccgcccgtcg
    21901 atcttcctca atacacccag tgcatccatc accgtattga tattcctgaa agtcagggtt
    21961 agtttgccca ctgcttctct catcctgcat gttgcagcgt ttgcctggac atatttcagc
    22021 acctgcgtga atatatccga ctggtaatag ggcgattcct ggttgctgac gaagtaacag
    22081 gtcatattcc cgttcctgag aatcatcttt tccataccca attccctggc cagccggcgc
    22141 atacggatag cattgaataa ttcctgggtt tcagccggta caggcccgaa acggtcaatc
    22201 attttctcct ggaaagtgag cagcaaatct tcatcttcga tgccgtcgag ctctttgtaa
    22261 aggctcatac gctccacatt gctggtgaca taatcgtcgg ggatcaggat ttcaagatcg
    22321 gtctccacct ggcattcttt cacaaattcc ttttcttcct catgcgcata caggtcgtgg
    22381 aactccgtat gcttgagttc ctgcattgcc tcatccagga ttttctgata catctcgaag
    22441 ccaatttcgg taataaaacc gctttgttcg gcgcccagga tatttccagc gccccggata
    22501 tcgaggtcgc gcatggcgat gttgaacccg ctgccgattt ccgaaaattc ctcgatggct
    22561 ttcagccgtt tgcgggcttc gtcggtcagc acgctcattg gaggcgccag cagataacag
    22621 aatgccttgc ggttagccct tcccacccgt ccgcgcaact ggtgcaggtc actaagccca
    22681 taatgatgcg catcattgat gatgatggta ttggcattgg gaatatcaag gccggactct
    22741 acaatcgtgg tcgaaaccaa tacatcgtat tccccttcca cgaaatcggc catgatcttt
    22801 tcaagtttca tgccgtccat ctggccgtgg ccgacggcga tcctggcatc gggaaccagc
    22861 ttttgaacga cgccggcaat ctcctggata ttctgtatcc tgttgtttac aaagaacacc
    22921 tgccctccgc gcgaaagttc ataagagact gcatcgcgga ttacctcctc attgaaagca
    22981 tgcaactcgg tctgtatcgg gtaacggttg ggcggcggcg tattgatgat gctcaggtcg
    23041 cgggcgccca tgagcgaaaa ttgcagcgtt cgcgggatag gcgtggccgt cagcgtgagc
    23101 gtatccacgt ttaccttcat ttgtttcagc ttttccttgg ccgcaacgcc gaatttctgc
    23161 tcttcatcta cgatcataag cccgagatcc ttgaatttta catcggcgct gagcaaccga
    23221 tgcgtcccga tcaggacatc gattttgcct tcctgcagtt cggccagtgt tttcttttgc
    23281 tgctgcgggc ttttaaaacg gttgatgtaa tcaaccctgc acggcatctc gcggagtcgt
    23341 tcactgaagg tcttataatg ctgcaaagca aggattgtgg taggaaccag tacagcaacc
    23401 tgtttgctgt cgttcacggc cttgaaggca gcgcgaatgg cgatttcggt cttcccgaaa
    23461 cccacatccc cgcaaatgag gcggtccatc gggaagtcct gttccatgtc ctgtttaaca
    23521 tcggaggtcg atttcacctg gtcgggcgta tcctcgtaaa tgaacgatgc ttccagctcg
    23581 tgctgcaggt aagtatccgg cgcgaaacca aatcctttcc cggcccgccg ctcggcgtat
    23641 agttttatga gatccttggc gatgtccttg actttcgctt tggtctttgc cttcagcttg
    23701 ttccaggaat tggaacctaa cctgtcgagg gatggaatat ggccttcctt acccgaatat
    23761 ttgcagatgc ggtgcagtga atgaatgctt acataaagca gatcgtcgtt ggcatagata
    23821 atacgaatgg cttcctgtat cttgccattg ttttctattt tctccaggcc gtcgtaccgt
    23881 ccgacaccgt ggtcgatgtg ggttacataa tcgccgggtt tcaggtcata caattccttg
    23941 agcgtaatgg cctgcttccc gctgaaactt tcgcgaagcc tgaatttatg ataacgttca
    24001 aaaatctggt ggtcggtata gcaagccagt ttggttcccc ggtcgatgaa gccttcgtgc
    24061 agcgaaagat tgatagacgc ataattgatg tcttcgggat tcaggccacg gtttttcagc
    24121 aggtcatcga tgatggaata gatgcgttcg acctgttgtg ggctgtcggc cagaagaagg
    24181 ttcctgatgc cgttttccgc attctcccga agattatcga gcagaaggtc aaaattttta
    24241 ttaaaggaag gttgagggac aatgtcaaaa ccgaaggaag catccgcggt gagggcatga
    24301 tgccggccaa actcaatgac gggaaattca ccaatgccat gaattacgga ctctttcccc
    24361 gtaaaaagtt cttcagggcc aagatgttcc acaagcccgt ccagttgttc aaatgccgtt
    24421 ttcgctttct ggtattcctt ttcgatcagg tcaacggcaa atgccatatc ctgaaaccac
    24481 agtatactct tgccgggaat gaattccagg aaagattccc ttttttcgtg taacacacgg
    24541 tcctggacgt tgggaacaat ggtgatgtgg tcgagtttat caatcgaaag ctgtgtagcc
    24601 gggtcgaagg aacggataga agccactatc tcatcggaaa attcaatacg aaaggggtag
    24661 tcgtttgtga aggaaaaaac gtcgataata cctccgcgga tggccatttg tcccggttca
    24721 gctacgaaat ccacccgtcc gaacccatat tcatccagca tatcgtacac aaaatccagg
    24781 tcaaccggct cattgcactt cagatgaagc gttgactttt cgaggtagtt tttggtaacg
    24841 actttctcgc tcaacgcttc aggccaggtg accaccactg tattttttgc acgggtgctc
    24901 aaacgcttca gcacctcggt gcgcatcagc acgttgctgt tgtcggtttt ttctatttca
    24961 taaggccttt tgtacgaagt agggaaaagc aatacccgct ttttatgaaa ggccgtttcg
    25021 gcttcaccca acaggttctc aagatcattg cagaaatata cggctgattc cttctcgctg
    25081 agaatgacaa ggtgaacaac cgggttagat tcaaacaatg cagcggtaac cagcgaaacc
    25141 gaagaccctg ccaacccgtt caggtgaatt cgctgcctgg caataccagc ttcgttgaaa
    25201 agagctgaaa gcgaaaccag acgttcatcg ctacgataac aggaaagcaa ccgggttact
    25261 gaatccgtat cctgactatt tactatgaga tcattcgttt caaagtcctg gtccatacaa
    25321 aacaacgttg gccgcccgga tgaatttatg gatttccggc ggcttgattg aagttgcaaa
    25381 aatacaggat taaatccaaa cgctgcagca ttatggccat cctattttta gcatgtcacg
    25441 ggcagcattt ttccagcata aaaacagagg tcacctggtt aatattggcc aaatctttgt
    25501 agctttattc cttcccgaac ctgatcatta accccctaac cgtaatacca tggaacccaa
    25561 aattgctcaa aaaggtcctt ttgccgttga cctggaagaa ggccagacct atcattggtg
    25621 cgcctgcggc cgaagtcaaa accagccgtt ctgcgacgga tcccacgagg gaaccggctt
    25681 tacaccggta gtcttcactc ccgaaaaagg cggtaaagca tacctgtgcg gttgcaaaca
    25741 ttcgaagaac aagccctatt gcgacggcac acataatacg ctttaacctg tatctgttac
    25801 ctttttaaag atatagaatg cttgttcagg ctcctgcccg ggcaggttga tgagcgtttt
    25861 catcatgacc ggtaataatc cctgtattcc gatcatcaga tacagttgtc ttgtcaatcc
    25921 tgattttatc tttcatgcca tgatcatctc cattgaaata agttattatc cgctccacgg
    25981 agacgatttt atcgaaccga tcaaagcctt tatcgaccgg ctgaattcgc accccgaaat
    26041 ggaagtccat accaatggga tgagcaccca ggttttcggc gaatacgaca aggtgatgga
    26101 aattctccaa aaagagatca gggcctcgtt tgagaaccct tactcagtat ttgtcatgaa
    26161 ggtggtgaat gccgatctcc gcgtgcacgc gaagcagtaa aaatcaaaat accggaaatc
    26221 gtcttaggtt atttttcgat ctctttgata atgctgcttc caaggatgtt ccagcgcaac
    26281 tgataaaacc tgaccaggtc aacacgcgga tcaacaactt gctcttcgcc tgatggcggc
    26341 tttgcatatc ccgaaaaatc aagctccgga tttttcgtcg ttaccatgcc cgtttgcctg
    26401 gctgttacca cttcggcgct tatatcgttg ccgccggagg ttgtaaaagt cacgattgct
    26461 tcatcaaccg ccggttttgc agggctgttc cagttgctaa ccaaaggcgg tgtttcggaa
    26521 attttgcttt cgttgccggt aattgctttg aagatatcgg tctggtcgta aagtacggca
    26581 tcaggatatt gccgggcgag caatccggct acgaaaccat aagccttcca gtttccgcgg
    26641 agatatatct ttttcaccgg tttcctggcc acggcttccg agctgtataa ccagaccctc
    26701 ggccccagag gctttttatc gtaatccagc cgggagccgt ctatattttt gtaccggtgc
    26761 gaaatattga tcgttcgttc aggaaccgaa cccgattcat actgatcaat cgttgatttt
    26821 attttcgaaa tatttgaaac gccgttttta tattcatatc ctttcagaac agaattgtaa
    26881 acaaggtttt cggcccaaac catgtcgtca tgctttgtat agcccaggtt gttatcaccg
    26941 taatcatagg aatcgcacga caacctgctg aatttgatat tttcgatcct gctcttgtcc
    27001 ttgttgaatt ttccctctat cgagatgtat tcatccgtaa atatacccga ctcaaaagtc
    27061 gcaaagaaat tttcgtttga aaaatttccg ctcaggagcc tgagagaaga gatgtacagt
    27121 ttgttttcct tatcctgctt ttttgagcca tcagcataca aaacatactg atcggactgg
    27181 cgcacttcct gtttcccttc ttcatccgta aaaacaccgt gatacctgaa atcgtattca
    27241 accgggatat caacctccac gctggtaagc cggcggggcg ccacagcttt ttgaaccgcc
    27301 accggggaag cgaaaccact attcgacagg ctggcgattt gttttgaaaa agagcctgtt
    27361 tcatcaaaag cggcagtcag aacagagagt attctacccg atttgctgtc gatcatgaga
    27421 gcattcaggg aagcatcggc tgagacatcg ccatcggaga tattagcgcc gcttaactga
    27481 ataatggtcg cggctttcat gatttttccg ccctcagctt ttgtttcggg cgttaccaat
    27541 cccgaaccgc ccaatgtcgt ttcctgtaag ataacgtcga tccgttgtct ttcaaacacc
    27601 ttaagcccgg gaaccgccgt aaggtctttc tccagaagcc cgcaagtggt gcattttacg
    27661 ttccctgacg ggttcccgaa taacggatta taataaattg cgactgagtt gggctcctgg
    27721 gccatcgccg tcatggatac tgcaatgacc gggacgagaa ggagctttga attcaagaac
    27781 gttctgatct tcatgttttc tgcttctgat tggtttagtg attctgttgt tttggaattc
    27841 tgttttaaaa gctgatacgg atggtataag ccgggattca tcagcatggc aggcccggca
    27901 tgatttttat aaagagacca gccacaacat tttagcggct ggtctctttc aggcaaatga
    27961 aaaactattc ctatttatcc ttgattatca gtttagccag tttttcaacc ttcacataat
    28021 cagtaaaggc gttggtaaat tcaacggtgt cgtttacagc ataatccttg atgctcgaca
    28081 atgtgaacca gccctgctga cctgcgtcca gaggcttgcc gtccttatca accataaggg
    28141 cattgatgat cttcggcatg acctgtccgc ccatcgtctt ttcaaccgga atgagcacct
    28201 gtactttggc tgataccttt gccatatccc attgcatttc agtgacctgt accgactgaa
    28261 ccttaaagct cttcccattc gtggaatcaa caaacggaat atcgatgggg aatttcatcg
    28321 aagctacagc gtccgaaaat tcctttttag ccgtttcctg cattttgttc atttccgcgt
    28381 tgattttcac caggtcatca gccgatttta cctctgtttt cgctttctct ttcaatgcat
    28441 cgtaatcagc atcgtatttt acctttatgg cagcggcttt cccgaaaagt ccttcggaag
    28501 atgctgcagt agagttttca gttttgttgc tgttgcatcc gaaaagcatc agcgccacaa
    28561 atgaagctgc gattagtttg gtattcatac gttaagtttt aattggttaa ttatttgatt
    28621 atgttgaata aaggcgctaa atgtaataat tattttttca gataatgata cttttcaaca
    28681 tttgatcaca aatatttttt tgtattgtaa cttaatatgc aaattccgca catgtttata
    28741 attcattcag acagagcctg tcggagtgac cgttttgcag attgtaatac aatctatccg
    28801 gataaatgga tatgaacatt gcactacatg gcgttgttaa aattaccggt tgccgtttac
    28861 gattactaat ctgcacgaaa cattgcatct ttgtgaaaac aggaatgtga ttgaccgtca
    28921 caaatgcaga cgaaaggcac aatcaacgcg gtgtttttca tgtaagattc ggttgcgctc
    28981 cccgataccc ggataaacca gatgtacaat aaaggccact atcccgctat tatggcattc
    29041 ctattcagaa ggaggaagaa attaacgtgc aatagccggc aacagcgaat tagggtccag
    29101 ttgatacagc agttttttga cataataata accgctctgc cggtgtaatt cttcctccat
    29161 cccgcttttc acaatgtctt taggcggaat gtccaaaacc ataatctgaa tagcggaacg
    29221 cggaaggctt ttattccagt atgccggatt tggacgcatc accggtgttt cattcttcac
    29281 agaaccaatt ccagttattg attccttgtc actgagatag gcaaacccgt ccttttcgtt
    29341 ctcactgaaa tggtcaaatt cttttgtgat aaccggtatc atgatttccg aagttatttt
    29401 atcgggcatc actttccagt agtccaggat cagcccgaag acttcgcgga tggtgacctg
    29461 ttcccagtac ggcggccgat cagggttata gatcaccacg gtgttgccgt aacggtcgat
    29521 gccgggactt aattcttccc taatgtcggg cagaaaaaac agttcgtgca gggccaaggc
    29581 cgcatcatcc atctttttcg gagtaaaacc cggattggtg gcatcatagg tatcgcggta
    29641 attcgtttca ttaaagccat ggctgcaaaa catatcaaag cggtttacat caaactgcca
    29701 gtaaggtggt tcgccggtaa atttcacctc tttcccatcc atcagcttcc aggtttgaaa
    29761 acagaaaact acttcgcagg ggattccgta cccgaattta acgaagcagg aactggcatt
    29821 gagaaggcct accccgtcga aacctttgat gttggaaagc acaggcgttt tgcgaaagat
    29881 accgatcagc gatccgaggt tttcggaagc cgcagcagct tctgcatccg taaaattgaa
    29941 cgtctttccg taagcgtcgg aatcctggcc attcagtttg ttcttagtta tcctgaaagt
    30001 tcccggtttg tccggcagca acatatcgtc ctgggccctc aacggctttg accataaggc
    30061 ggtgattacc agcagaacca aataaaaaaa attgtatttc atggtgtttc gcatcatata
    30121 gttaccaaag aaaaaggcat ttaatttcat ttgtcaaaac ccaacggatt gtaagtcagc
    30181 tcggtggtac ggccaacatc aatacccgtt gctgctgtga ctttggcctc gaatgcctgt
    30241 ccaacctcca ttttccccac tccggcggtg agcgtagcct ccgatttgaa acctaagtcg
    30301 ctaacctggc tcttggtgtt gtaagtaatt tcgaacccgg tcttaccttc cgctttgaca
    30361 ttcactccgc cttcgacgcc caaatcggct ttcacgccgg ccccgaagaa cccggtaatt
    30421 tcttttttct tataattcca ttttgccccg gccaaaaggc cttcgccgca ctcgccttcg
    30481 atgctttcgc agtcaagccc gaaactgcat acaatcaatc ccactttaag tttcttgccc
    30541 tcaaaaggac aatcggggac ttttttatcg ggaacattta cctcgttatc ctctgcttgt
    30601 gacgcaggcg gaggcggggg cacactcggg cagtcaccgc caaccgaagc gaaagccgtc
    30661 atattcagcg aggcaaaaag caattgtcgc acctgatatt catagtacaa tgtgtagtag
    30721 ctggaataga cgaaggcttt cctggcattg tttaatttcc caaactcatt cagatcatag
    30781 gtcctgttca gatactgctc gcagtaaacc cagtaagtag tgagcagatc gttatagcgg
    30841 ttttgccgcc gggctgcatt gtcgcgccat tctttgtaat tatcatccac caggttggtt
    30901 atctctttgc acctttctat caggaaagcc tgtatctttt caacatcacc gtccagcgtt
    30961 ttcattttgg catcattggc gaattctatc tgttcgagcg acttcatgaa cttagcattc
    31021 gctaggctgt cacttttaat atagcgcttg ttttctttgg ctatctgatc ttcaaaataa
    31081 gccttgagca gttccatccc gaaagcggta ttatcaaacc taatcaagcc cggggtggtt
    31141 tctttctttg tgaaagatct ggcccatgca gcttgctgtt cgggtgtcat atccgctaaa
    31201 ccggcgaaat ctcccagcga cttttcctgg gattcatccc ggccttcctg aagcactttt
    31261 atcagatcct tgatcgaatt atcatggatc agtgcagata catccggcca ttgagggaat
    31321 gagggaagat gcagattttc gaccgggaca tccggattcg gcgtaccgga accttcaccc
    31381 gcggaaggat cagtgccggg cttgttgccg ccaggttccc cgggggcctt atagtcatcc
    31441 cgggacttca aattttcatg cacggttttc atcgactggc aatagatccc ttttgcccct
    31501 ttgtataatt cttccaatgc attcttaaaa tccttccttt tcaggtaaac attaagcagc
    31561 ccctgtcggg caagcgcaaa gtcggggttg attttcaacg ctcttttgta gaatgcctcg
    31621 gccgaacggt catcatacag ttcaaaaagc gtatttccca gattggtgag gataaccggc
    31681 gccgagggat agagcgattt ggcgtagagc aataccggaa gagaagtttt taacgaatcc
    31741 atcattctca gtatggcccc gaaattgttc acaatgaggg tatccttcgg aaacaggata
    31801 acagcttttg ccgaaaaggc gagtgaaatg ttaaaggcct gccccgtacc ggtgaggcag
    31861 actgccgtgg aacttagccc ctgagcaatt ttatagcgat cggcgccttc cgtttccagc
    31921 tttttgaagt tatcatctac aaacttcttt tcgcttccgg cgtaaacggc tttataatgc
    31981 cggtcgtaga aacgggtagc tattttaacc atctgagctt ccgaaatcac cttcgttgta
    32041 tcaaccttaa cgtacttgta catcgtggcg gattgacctg atacccggcc aaccaagaag
    32101 aacattgcaa gtaaaaccgc taccggtaaa atcgtactat aattgttaat cgtcaaatac
    32161 gttttcattt ttaccgaaag attggatgga tattctgtga ggtctgtgtc aatttataac
    32221 aatacaaata attttactca ttcaaaggat tctaccgtga acaactatat ttactaatca
    32281 gatgtaggcc cgtggattca ggaattcaac aatgcataaa tcaacgcttt attattttga
    32341 aaaactgccg gctttccatt ataatccgac tcctatcaca tttttcgttt ccatctttcc
    32401 gccttccatc acggtggccg acatttccat gttcttcccc actttcaccg ggccgcctac
    32461 ggtggcactc atctctacct tgccgccaat atccgtgtcg ccgccatcgg tccgcgtcag
    32521 cgttacgccg gcctttaact cgcctccggc aactcccagg tgcgcctcgc caccggcgcc
    32581 tacaaacagc gtagtgctct tattcttaaa attccttttt acagatacgg cagcccccgc
    32641 cgaacaacca aactcaatgc tctcgcaatc gaaatcggca ctgcacgcca ggaaaccgag
    32701 cgaaagcttt gaatcttcag gacaaggatt ctcccggggc tttttggtct ctacctcttc
    32761 ggcaggaatg ggataaggat tggcatacaa cgagcaatcg gtgccgcaat cgttctgcat
    32821 cggcgccatg aaggcctgag gataggaacc gtatgcattg cccacgatcg acaaggccac
    32881 acgctgcact tcataagcat agatcttatt ccagtacggg ctttcaattt tcgacaacca
    32941 ttgcccggaa aaggcataca gatcgtcgag cagtttctcc tgctctttca ccatatcccg
    33001 gaattgcttg cgcgaatcct cgaaataccc ctgatgcttc ccgttggtaa tattggcagc
    33061 cgggcactct tccgaacagt acttcctgtg acactcttca atacaatgtt cttccgcccc
    33121 gcagccttcc acacagccca cgtatgtctt caccagccgt tcatgctttt ccaggtaagc
    33181 gtttatctca tcataatcct tcttcatgat ctcatcgatc ttttcctggt agcgtttatc
    33241 cactttatcc gattcctgga agaagtattc ggtgatgcag tcgagggcaa accgttcgtt
    33301 cgggtaatcg cgcaggatgg cattgggagg aagtgacggg agttcggtat ggacctgaag
    33361 gaattcattg gaaaaggcca tcagcttcgc gtggaacctg ccatatgcat caacggcaga
    33421 agaataaccc cctccttccg tccagtcggc caccttatca caattgggaa atgacggcat
    33481 tttgatacgg tcatcttcag gcaccagcga agcaagtggg tccggtggtt caagctggtt
    33541 gcgggtctca tcccagaaat cttctttcgc actctggttg cctgcctcgc ccgcatcctc
    33601 cgcctgcttc tgcatgtaag cgtaattgtt cgaggctttg gcatacgaca ccccaattcc
    33661 cttcactccg gcgaagagct gcccgatcgc ttccttcaac ctgttctgtt tgatgtacag
    33721 atcgcagagg gcggagtggg cttgtccgaa gtcaggattg tattcgattg cctgcttcag
    33781 ataattctca ccggtttttt catcattcag ttctatgtag ctgcatccga gttgtgtcag
    33841 gatgacaggg ctctgaccaa aaagtgaatt tgcatagagc aatacgggta cagaagcttc
    33901 aatcgaatcg atcacgcgga ggtagccccc gaaattattt gcgctaagcg tgtcggtcgg
    33961 cctggctttc aatgctgccg tcaaatacac acaggccagg ttgtgatcat tgcccaatgc
    34021 agtgagggtg gctcccgccg atgtcagctt cctgactgcg ttgaagctga acttgtcgta
    34081 ccccgcctgc ttgtagtcca ggtcgaactg ctgcttcgaa atggcatcca gggctttgta
    34141 agagcgggca tagaaccggg aggcgatggc cagcacattg gcttcggtag caggagattt
    34201 tataagtaac tctcctggcg tttttgttgg ctgcgaagcg tttcccgccg gtttcaccgg
    34261 ttgcccggtc atctgtctgg ccagtttctg gatctcctcg ttggcttttt tggcagctgc
    34321 cggatcattc cagttggtgg tctgacggat cttagccatc tgctttcgga tcgcatccgg
    34381 actgctgttt tgcgaaagag caggaaatgc tatagcaaac agtaatacga ggtaaataaa
    34441 acgattcatt ccgaaacagg tgtttaacta gctatttata gtaatcaatc tggaaatcag
    34501 gcaaatcgat ctgggacctg aaaatgaaca aatcgtatgc agacatataa ttttcaactg
    34561 cctgaagcga gaaactccct tttgcattag ccggaaggga aagatttgta ttggtggttg
    34621 ctgcggtagt ctgaatctgt aattcttcgg ctttgtcgta cagaggcagg ttgccgacta
    34681 aactttcctt ataccagttt aggtatttct ccagtttagg tgtgaatttt tcgcaatacg
    34741 cccgataaga cttttgcatt tcacggattt tcttaacaac tgcattccaa tggtttacat
    34801 ctgcctgagg aggtgtgcca cactcattac aatcatatat tgagcgaatg ctatgcagtt
    34861 catttaccaa ctttttcaac tccgaatcaa gagccgcttt ttctctcact gcttcatcca
    34921 gaagcgcctg ctttcggttg atcacatcca tctcataatt cctgattttt ccttgcaccg
    34981 attgttgctc ctccatcaga ctcagcattg aagcggcctg attattttgt tgtgccatct
    35041 ttgcattttg tcccgggttt gcctgagcat ttgccatttg ttcagccgcg tatgcctgcg
    35101 cccacgcttc ctgcccttct ttagtcatat tctgcatatt tttcacttcg tcgagcgaca
    35161 ggttttggtt ttgcagtact ctgtctgcca aagcatcttt ttcggcttgg gtcatatcac
    35221 cttgtttatc tttcagcttc tgtatctctt ccatgcttaa acccgatgtt tccgaaacac
    35281 gttgcatcat tccattttcc atgctgcctt tattcccctc gatatagctt tccaattgat
    35341 tttttctgtt ctctgcatcg tcagtaactt ttaccttaag gtcatcaacc agttcggcat
    35401 attgctgcat ggcttctttc gaacaagagc aaaccgaatc aggaaatttg ggcattttat
    35461 tcaggtattc caaggcagtc tgtgcgtttg cagtgtttaa tgctgacaca ataactactg
    35521 cagaacaaag ccggatgatt gttcttattg ttttcatagt atcttaggtt tagaagccga
    35581 ttacatcttt atgaattcta ccctgcggtt aagggctttg ttggccggtg tatcgttggg
    35641 ggcaaccggc tggctttcgc ccatgccgtc ggttaccaaa cggtcgccgt ttacattgaa
    35701 cgattttacc agttcatttt taacagaggc ggcacgacgt ttcgaaaggt cgaggttcga
    35761 ggcatcggcg ccatcgctgt cggtatgacc gacgattttc acctttacat cgggaacttc
    35821 gttcaggata gccgcaattt ccttaagtgt tccgtatgat tccggcttta ccacatcctt
    35881 gttcacatcg aaatagatgc cgtaagttac caatttacct tcggtcatga gtttgttgcg
    35941 catatcggga gcgccgactg ctacccggac attgctgatc atagcagcgc catactcgaa
    36001 cctgagcctg tcgggtttca cgcaacctgt agggaaggcc ttaggagcat cgaatagttt
    36061 gtcctggttt tgatagatac ggaccctgga tttctgaacc cagatagcta tatggtattt
    36121 ttggtttatc ttttctttga acgcgtcacc ttctttatac ccgttcaact ttgccatttc
    36181 acattcctga ccatatagcc aggtatgata actcggcctc ccgaaatact caatggagaa
    36241 ctcaatcccg ccgttccccg gagccgaacc tgcatcccag gcttttgcat ttcttgcttg
    36301 cataagccgc atattccatc ccgacatatt agcattctcc aggccaccta tgggaattac
    36361 atcaaattcg acagtataat tttcaggcaa cttgagcaaa gcatcggtcc acacgcattc
    36421 attcataaca aacttcatcc agttgccggg gtataggttt gtggtgacta cttcggccga
    36481 accgttggtg ttccataatg ccggaaaatc accgacagca tcctggctga aatcctcgaa
    36541 aaagatcacc ttttcaccgg gaacgaagtc gtatttggag tatgcttcca aaccggcttt
    36601 accttccttg ttatctcctt ttgtgtcctg accggtattg tctttgcctt cctccgcctc
    36661 attattggtt ttgttctcct gctttttatc ccctttgata cttttttcta tcccatcaag
    36721 ccccttgtcg atcccccggt cgattttgtt gttgatgcgg ttgtttactt tgttctcgat
    36781 ttttttgcca acgttgaatt gcccggaagc aaacgtaacg ctgaacatga acactgccag
    36841 gagcaatagt ctgaagatgc ttttcattgg tttcaggatt aaatgattaa tgcatgatga
    36901 cttacagaag cagtacaagt atggagaaga acgaggagag ttattagctt ttaattcaga
    36961 taaggcgttt tagattttta tagtttcagg aattcgaccc tgcggttcat ggctttgttg
    37021 agcggggtat cgttaggggc tacgggcagt gattcgccct tgccatccga ttcgaggcgc
    37081 gaggccgcga taccgaaggc tttcaccagt tcgtttttaa cggaagcggc gcggcgtttg
    37141 gaaagatcaa gattggcggc atcggccccg tcgctgtcgg tatggccaac aattttgatc
    37201 ctgacatccg gatttgcttt gagtacctca gcaatgctgt ttaaggttcc gtatgattcg
    37261 gatttaattt cggctttgtt tacgtcgaag gttattccgt aagtaaccag tttcccttcc
    37321 gtgattaatt tgctccgggt atcgggcgag gcggtggtaa ttttaatatt ggaaaccatg
    37381 ggccaacttt tggcatccca gccgccaaaa cgcatccggt tgaaaacggt gccctcaaaa
    37441 atattggtcg gcacatccag cgttttggcc ccctggtgat aaatcctaac ccgccgcttt
    37501 tgtatccata taatcacatg gtttacctgc tcttttaaaa cagggttttt aactgactga
    37561 ccagtcatat ctgcgttgtc ttcgttacgg taacctttcg tctcccaccc gtccgatttc
    37621 agattgatgt gtagcccttc gttgccaggg aaaaggtcat cgttcatttc ctttgagtca
    37681 tccggatttt cttcgtataa agtgaacgtt atcccgtctc tgtattcatc atcgggaata
    37741 atatcgaatt ccacgatgaa gttttcaggg aattttatgt ttttcgtata gcaatatgca
    37801 gcatcttcac cattaaggtg aaaccatttt ccgggagcga tatttacggt ttttacttcg
    37861 ccgctgccgt ttgatgtcca caacgccgga aaatcaccga tggcatcctg ggaaaaatct
    37921 tcaaaataga gcactttatc gcccggaaca aagtcatatt ttgaatatga ctgcaacgaa
    37981 ggctgatcct gtttccctga tttcggtttt tgggtgtttt catccgcttc ggaggaagag
    38041 ttttcatctg cgttttcact atcctgatct tcttccactt ttttactgtt tttattctca
    38101 cctttcaggc cgccctcaac ctcgtcgagg cctttatcga tcccttcgtc gattttgtta
    38161 ttgatccggt cattaacctt gtcctgaacc ctttcaccaa tattaaactg ggcattagcc
    38221 acataaaccg gcaatacgag tgcaatcagc agcatcagtc gaattagagc tttcattgat
    38281 tatattcttt atgttagtat ggttgagtat gtttgatcgg atttcagatt gcgtaaaagt
    38341 aaagcaatct tacgaatttt attaaaagtt tcagcctgcg ccggcaattt ttcatcacaa
    38401 accgacaaat cgggataatt gatcgaaaca gaaacctcac cccaacccct ctcctaaagg
    38461 agaggggctt ctcctcaggg agaggggctt ttcctaaagg agagtggctt ctcctcaggg
    38521 agagcgtcct gcatcttctt ccagttaagt ttccggctta aattgttgat tcctcgccgg
    38581 tacctatgga tgattttcat tgaatcctcc gctcctcaat gtatgctcaa tgctgtttca
    38641 acctcctgta taaaaccggc ggaccagcct tctgcatgca gccgtcaagg cgcttttaac
    38701 gcgccctgct gaaatcccct gaatattcgc gcatatagga ttctgctttt tcaaccattg
    38761 atttggaacc gataaagatg ggcgtccgct gatgaatatc atacggctcg atatccatga
    38821 tcggcgtaaa accgtcggat gctttcccgc cagcctgttc gatgatgaaa gccatagggg
    38881 cgcattcata cagcagccgc aatttcccat gagggttctt caggttgccg ggataaatga
    38941 agataccgcc acggagcata ttccggtgaa catccgatac cagtgagccg atatatctcg
    39001 tactgtaagg ccggtttgtt gcctggtcat attcctggca atatttgata tactttttca
    39061 ccccatcggg gaagttgata tagtttgctt cattgattga ataaatcgtc cctgaatcag
    39121 gaatacgcat gttggggtga gacaaaaaga acaaacccac ggcggcgtca taggtaaacc
    39181 cgttcacgcc atttcccgtt gtatatacca ggatcgtgga gcttccgtaa ataatgtaac
    39241 cggcagccac cattttccgg ccgggctgca acaaatcatc cacggtagcc gaagtgcctt
    39301 cgggcgaaat cctcctgtat atcgagaata tcgtaccgat cggaacattt acctcaatat
    39361 tgctcgatcc atcgatcggg tccatcgcta caacatatct cgccttttgt gaataggaat
    39421 tgccgaaaat gacaagatct tcgttttctt cagaggcaat ggcggcacat tgccctccgt
    39481 gtttcagcgc attgataaac tggttgttgg catacacatc cagcttcttc tgttgttcac
    39541 cctgaatatt ggtagtgccg aacataccat tgatatctgc aagtcccgca atccttattt
    39601 cacggttcac gatcttggct gcaatggtaa tatcatttaa taaacgtgta aattctccgg
    39661 ttgcaccggg atattcgatt tgcttgtcat tgataaactc aactaaagtt ttcatgaatg
    39721 atgaggtttt atgaataatg ggaaatgtcc taaaatcggt tgaaaggttt ttgtatgtca
    39781 ctgatagtaa ataccaggca gaatagcagg cgcaaaacag tagcttgaac aaatgtatta
    39841 cttttacggc gtttttcgcc atacagcagc acatgaaagt tttcaaattt ggcggggcct
    39901 cggtgaaaga tgcagccgct gtcagaaaca tagcttcaat tatccgcaat tatgctacgc
    39961 ctcagctcgt ggttgtaatt tccgccatgg ggaaaaccac caatgcgctt gaaaaagtgc
    40021 tccaggcctg gtactcgggc agtgaaaacc tgcaacaata tgtggatgag gtgatcgcat
    40081 atcatcagca gattatcggc gacctttttt ccgacagccg ccacccggtg catttcaaga
    40141 ccggcttgct cttcggtcaa ctggaaggac atctgtgcac cccgccttcc ctcaactttg
    40201 attttgagta tgaccaggtt gtcagtctgg gtgaacttat cagcaccact attgtgagtg
    40261 aatttcttgt aaatgaaggc tttaactgca aatggttcga cattcgggaa attctcttca
    40321 ccgacgaaaa ctggcgcgaa gccaacgttg actgggcgaa aaccggggaa aaagtcaaac
    40381 agcaaatccg tcaattcctt ataaacgata gccctgaacc gcgcattgcc cttacccagg
    40441 ggttcatcgg ttcaaccgct gacggcgaca ccaccaccct cggccgcgaa ggcagcgatt
    40501 acacggccgc tatacttaca catctgctgg aagcagatga aatgaccgtt tggaaagatg
    40561 ttcccggaat cctgaatgcc gatcccaaat ttttcgataa cacctcgctg atcaaccgca
    40621 tttcgtaccg ggaagccatc gaactgacct attacggcgc caccgttatc cacccgaaaa
    40681 caattaagcc ccttcaaaac aaaggcattc cattaagggt gcgttcattc gtttcgccgg
    40741 catcccccgg cactattata ggcccggctt cttcgggcga cgatgaccag cccagcatca
    40801 tcctgaaatc aaaccaggtc attttgagtt tctctccccg cgatttttca ttcattgccg
    40861 aggaaaatct cagcgaaata ttctcagccc tggcttcaag cagcatccgt gtcaatatga
    40921 tgcagaattc agctataagt ttcaccattt gcatggacga aaacgaacgg aagatggaaa
    40981 aactcaccgc attgcttggc gaaaaatttg cctatcgcta taacaatggc ttacagttaa
    41041 taaccatccg gcattatgac caggctacga taaaccgtgt tctggccgga cggcaattgc
    41101 tggtggaaca gcgaagccgt caaacagcgc aatatgtggt aaagacgcag gattgatatt
    41161 caggaaggcc tgaacgcctt actgtatcag cacatgtttg gctgttgctt acattatttt
    41221 caaaaacaat cgtttatcgc cggcggcttt tcaatttatg attaatttcg ccggctgctg
    41281 taaccgacgc caggctaaag atcagcacgt taacccggaa ttcgggataa gtaacaaaca
    41341 gcaaactgaa aaaaaacatc actccatgaa aggatcggtt ctcatacttg ccggcggcgg
    41401 gccggcgccg ggtattaaca cggtgatcag ttcggtagcc aaggtcttcc tgcgcgacgg
    41461 attccgcatc attggcctgc acgacggata taaaaaccta tttaccgata acccttccac
    41521 catcgacata gatttcgaaa cagccgaccg cgtttataat gtcggtggtt cgatattgcg
    41581 gatgagccgt tataagcctg ccgatctgga atttaagacc gatttcttta aaaagaatga
    41641 tgtcaaactg ctggttaccg taggtggcga tgacaccgct tccacggcta accgcatttc
    41701 aaagtttttg gccgagaaac atttcccgat ccagaacatc cacgtgccca aaaccattga
    41761 taatgacctt cccctgcctg agggccagcc tactttcggc taccagagcg caaaacagga
    41821 aggagtgcgt attggacgta ccatttatga agacgcccgt tcctcaggaa actggtttgt
    41881 tgtttcggcc atgggccgcg aagccgggca cctggcattc ggtatcgggg cagcttgcca
    41941 ttaccccatg attgtcatcc ctgaattgtt caatcatacc aatgttaccc tcgataaaat
    42001 tacacgcctt atcgtttcat ctatcattaa aaggaagatc cttggcattc cttacggcgt
    42061 cgctatcgtc agcgaagggg tattccactc gatgagcgat gacgaagtgg aagcttcagg
    42121 aatccagttt acctacgatg aacatggcca tcccgaactg ggaaccgtga gtaaggcgca
    42181 tatctataac atgctccttc agatcaaact gaaacagctc ggaattgacg taaaaagccg
    42241 ccccgttgaa atgggttatg aactgcgctg cattcagcct accgctttcg acctgctgta
    42301 ctgcagcctg ctgggcatcg gcgttaaaaa gctcttcgac gaaggcctta ccggatgtat
    42361 ggtaaccagc gacccgacag gcgacattgc cccgttattc ctgaaggatg ttgaagataa
    42421 actgggtaag gtcaaaaccc gtatggtaaa tatggaaagc cagaaagccc gtatggtttt
    42481 tgaggacagc atccagtaca tcaccgagga agactacccg gctgcgtcga agtacctgaa
    42541 aaatcccgaa gaatacgact tcaggaaaat actgaaatgg gaataaccca gggaaaatgg
    42601 ttcccaaaac ggttccgggt ttaatccgtt acgcggtaaa tcataacctg atatcaaaag
    42661 aataaaattc tccggctcag attccggaat ccctgctgcc ggcttccgca tcccggcttg
    42721 cggctccgca tgcagaacct atgtaaggta tcagataatc ctgaatcaga taaaatgagt
    42781 actttcgcgt tcaattaatc tgaacttatc acaactgcgg atttgagtcc cgtcctcctg
    42841 ttatgtggaa tcaccttgtt agaataattt tccgaaaccg cccggcaatt ctggccggaa
    42901 tatttattat cactgccctg atggcctggc aggcttccga cgttaagctg tcgtacgata
    42961 tgcttcgcat gttgcccgaa aacgattcca gctatgttga ttacaaagaa tttaaaagtc
    43021 gctttggcga agacggcaat gtgctcgtga tcgggattgt caatcccgat atgtttaagc
    43081 tggatgaatt caacgcctgg tatgatctcg gcaacgaaat caaaaaaatt gacggaatcc
    43141 aggaagttgc ttccatcacc cgcgctataa gccttaagaa gaacgacagc atccataaat
    43201 ttgagtttct gccggtagtt gcccggagag cgtcatcgca ggccgaagtc gacagcctga
    43261 aggagatgat tcacggcctg aaattttatg aagggcttat gtacaataaa agtacaaatg
    43321 cttacctgat ggcggttacc ctcgataaaa agaagcttaa tgacaagagc cggaccgctg
    43381 ttgtgactaa aatccacgaa gctgccgaga gataccataa tagtacaaaa cacgaaattc
    43441 attattcagg gctgccatac atccgtacca tccagatgga aaaaataaag aatgagcttt
    43501 atcttttcat cgttttatca gttgttgtcg ccgccttcat tctcttcctc tttttcaggt
    43561 cgtttaaaat tgtgctcagt tcgctggttg ttgtcggcat cagcattgtt tgggcgcttg
    43621 ggctgatagg cctttttaat ttcaagatca cgatcctgct cggggccgtt ccctcgctga
    43681 tcgtcgttat cgccatcgaa aactgcattt atatcgtaaa caaataccat tgggaatacc
    43741 gcctgcaccg taataaaatg aaagctcttt cccgtgttgt acaacgcatc gggtatgcct
    43801 cgcttatggt taatacggcg acagccatcg gattcgcggc gttcatcatt gtttcgaacc
    43861 agatgctgcg gcaattcggc atcattactt ccatttgtgt aatcatggaa tatgtgctga
    43921 ccatcaccct gctgccgatt atcttcagtt ttctgaaaac gccggatgag aaaaatgtaa
    43981 aacatcttga caataaattt ttcggcagaa ttctcgataa aatcatatat ctgatccagt
    44041 tcaggcgaaa atggatatat ggctttgccg ggttgctggt gcttgtcgga atcgttggta
    44101 taacaaggat ccggacttca ggcaaagtcg ttgatgattt gaaaaagtct gatcctattt
    44161 cagccgatct gaaatttttc gagaaaaact tcggcggcgt gatgcctttt gaaatttcca
    44221 tcgatacgaa aaagaaaaaa ggcgctatgc gtatgcaaac cattaataaa attgatgagc
    44281 tgcagcagga ggtgcttaaa cataaggagt tctctaaacc cctttcgctg gccgaagcgg
    44341 ttaaatttgc caaacaagcc tatttcaacg ggaaacccga gtattatacc ctgccaaact
    44401 cacaggagcg aaattttgtc ctgtcctact tcccgaaaaa aagtgaaggg aaaaccaacg
    44461 tcctgaaatc ctttctcgac agcacccagc aaattacgcg tgtcagcttc cagatggccg
    44521 atgtaggcac gaaagaaatt aaccgtctgc tcgacagcat caaaccgcag gttgaatcta
    44581 tatttaacaa ggaagattac accgtgagca tcaccggcaa cagtattgta aacgcacgca
    44641 gcaccgaatt cctcatcaag catttgtttt tgagcgtcgg tattgccatt gcactgatct
    44701 cgctgctgat ggcgctaatg ttttccagcg taaggatgat tgtcgtttcg atgctcccga
    44761 atatcattcc tctgctaatc actgcagcca tcatgggttt cgccggaatc cccatcaaac
    44821 caagcaccat cattgtgttc agcattgcgc tgggtatttc ggtagataat gccatccagt
    44881 atctctcccg ataccggcac gaactgaaag taacgggcgg caatattaag ctctctacca
    44941 ttaatgcact gagagaagcg ggtttcagta tgctttacac ctccattgtc ctggttcttg
    45001 gcttcagcgt atttatagtt tctgaattcg gcggcaccca ggcccttggc atcctgattt
    45061 ctaccacgct tttcattgcc atgtttttca atatcattgt actgcccacc ttactgcttt
    45121 cacttgataa gttcatcgtg accaaagcct ttgaagccaa acccatcatt gagatttacg
    45181 acggcgacga tgtcaacaac ggcgatttgc cggttccgga aaaataaccg gaccggaata
    45241 agatatgcat tttacccgtg tttatagttc atacatcatc ttattaaaga acacaagcaa
    45301 tgaaaggaat tatactcgcc ggaggagccg gcacccggtt acatcccata accctggcaa
    45361 tcagcaagca gttgatgccc atttatgaca agccgatgat ttattacccg ttgagtgtgc
    45421 tcatgatggc cggggtccgt gatgtgctca tcatcagtac tcctttcgac ctgcccggct
    45481 ttaagaggct tctgggtgac ggcagctcac tgggatgtaa tttttcatat gccgaacaaa
    45541 aggttcctaa cggactggca caggctttcg ttatcggcgc tgattttatc ggtaacgata
    45601 aagcctcgct cgtgcttggt gataatattt tttacggcac cggcctcgca aaattgctcc
    45661 aggcaaacaa cgatcctgaa ggcggcgtgg tttttgccta tcatgtatcc gatcctgaac
    45721 gctatggagt tgttgaattt gaccaaaaca acagggccat ctccatcgaa gaaaagccga
    45781 aatttcctaa atcgaatttt gcagttcccg gcttgtacct ttacgataac tcggtaatcg
    45841 aggtagccag aaatatcagg ccaagtgcgc gcggcgagta tgaaattacc gatgttaaca
    45901 ggtattacct cgaacagggg aatctaaagg ttgcaacatt aagccgtgga acggcctggc
    45961 ttgatacagg aacgtttact tcccttctgc aggcatccca gttcgttcag gttatagagg
    46021 agcgtcaggg gcttaaaatc ggctgtatcg aagagatggc gtaccggatg ggattcatcg
    46081 atcgtcgtca acttgaaatc ctggcccaac cgttgttaaa gagcggctat ggaaattacc
    46141 tgctcaacct tccggttccc gaacgttaat ccgatatgca tacgttagaa accatacatc
    46201 agcagataag cctggctatt gaaaagcttg atattcccgg taagccggcc aatctgtacg
    46261 agccggtttc ctatacatta tcccttgggg gaaaaaggct tcgccctgcg ctggtaatga
    46321 cggcctgcga catgtttggc ggcagcctgg atgaagcggt ccacccggcg gtcggcatcg
    46381 aactgtttca caatttcacg ctgctgcacg acgatatcat ggatcaggcg cctttgcgcc
    46441 ggggaaagga aaccgtttat aaaaagtgga attcaaacat cgccatactt tcgggcgata
    46501 ccatgttcgc catggcaaac cgggaaatac tcaaaaccag cctttccgct gtacgggaga
    46561 tcggggatct gttcaattta acagccatcc aggtttgtga aggccagcaa tatgatatgg
    46621 atttcgaatc gcgcgacgat gtcacactcc cggaatacct tgagatgatc aggttgaaaa
    46681 cggcagtgct gctggcttgc agccttaaaa caggggcaat aatcgccggg gcatcccccg
    46741 gggactgtca attaatatat gatgcaggta taagccttgg aatggctttt cagttaaagg
    46801 acgatttcct cgacacttac ggtgatgaaa aggtttttgg aaagactccc ggcggggata
    46861 ttctggaggg gaagaagaca ttcctttttc tgaagctgat tgaaaaggca ggcacggaag
    46921 caaaaacctt taaccgacta tactctccgg cttcagcatt ggcggcggct gaaaagatca
    46981 gattcttcac cataaagttc ggcgaatggg gaataagtga agatgttgag cgtgaaattg
    47041 actcctttta taaatcagcc cttctcagca ttgacgcttt gagtgttgat tctgtgcgca
    47101 aatccgtcct tttggaattg atccggaaac tttccctaag gcaaaaatag ccctgatcgt
    47161 cctgaaaccc tgacagtgtt cccgtcaggc attttctttc tgtaactttt cgtattcatc
    47221 ctgggccgac gaccatttct ccaccttttc gtcgagtaat tccttaagcc ggttatattc
    47281 tttaaataat ttagcgctcg cagccgaatc ggtatggttg gcagggtcat tgagtatctg
    47341 gtcccaatcc ctaatttcct tttccatctg ccctatttca tcttctagtt tctttaccgt
    47401 cgtggcagct tttctcaatt cacgatcctg ctgttttttg cgctcgtaat ttaccttgtt
    47461 accggtgatt gtatcttctt ctttctgcct ttcggcattc atcttctcca gctctttgag
    47521 cgattgtagt ttgcgggcat tcaggaaatc ataaacgtcg ccgaggtagg gtttgatggt
    47581 atggttcctg aattcaaaaa ctttatcagt taagccttgc aggaaatcgc ggtcatgaga
    47641 aacgatgatc agcgtaccgt cgaaccttaa aagggcgttt ttcaggatgt ccttgctcac
    47701 catatccagg tggttggtag gttcgtcgag caccagcagg ttaacaggct tcagcagaag
    47761 ctgtgctatg gccagcctgg ctttttcacc tcccgacaat acttttacct ttttatcaac
    47821 ggcttcgccc ccgaatagaa agctgcctag tatgttcctt accttcggcc tcatatcgcc
    47881 gacggcgata tcgtcgatgg tctggaaaac cgttttttcc tggtcgagaa gatcggcctg
    47941 gttttgggca tagtaaccta ttacaacgtt atggcccagt ttacaaactc cctgatgcgt
    48001 caggttgcct gtaataattt ttgccagcgt agtcttccct tcgccgttac gacccacaaa
    48061 agcgacgaaa tctccttgtt ctatggcaaa ttcaaggtta ttgagaacca gtttatcgcc
    48121 gaaacgtttt gtaagggaag cagcttcaac cactaccctt cccgaccggg gcgcaggcgg
    48181 aaatttaaaa tggatagccg aagtatccac ttcgtcaagt tcaattttat ccattttatc
    48241 cagcatttta atgcgcgact gcacctgccg ggcttttgtg gatttatacc tgaacctctc
    48301 aacaaatttc tcaatctggg ctatctgctg ctgctgattg tcataagccg ctttctgaag
    48361 ttccatctgt tcggaacgca acagcacata ttcagaataa ccggctttaa aatcgtaaat
    48421 ccggcccgac atgatctcaa tggtccgggt ggttacgttg tcaagaaaag ccctgtcgtg
    48481 cgaaacaatc atcactgctc ccctgtatga gatcaggaat tcctcgagcc attgtatcga
    48541 ctcgatatca aggtgattgg taggttcgtc gagcagcacg acatcgggat tgcgaaggag
    48601 gatttttgcc agttcaacac gcattttcca tcccccgctc aacgtattca tcggccggtt
    48661 aaattcttcc ggatcgaaac ccaggccttt cagcactttt tccgcctctt cccttaccag
    48721 attgccgcca atgaggtgaa aacggtctgt agcgtcgtga aggcggttta tcaggttaat
    48781 atatgactcg ctttcatagt cagttctttc gccgagggca gttgttaatt tttcaatttt
    48841 ctcatgaagc gacctggcct ctgtaaaagc ggtcaaggct tcatccaaca cacataattc
    48901 gcttttcgaa tcaatctgct ggggcaaata gccaagggtg cttcccgaag ggcttgcaac
    48961 aacgccgttt tcgggcgaca gctcaccggc tataatgcgc atcagtgtcg ttttaccggc
    49021 gccattcttc ccgacaaggc cgatacggtc acgatcgcct atcaggaaag atacatcttc
    49081 aaataaataa tcaccggtaa aatggacaga aaggttgctg acggatatca tggtgcaaag
    49141 gtagaagttt ataccagaat aatttttgat gttgctaaaa ctggttggta ttaaaagaat
    49201 cgtcaggctc aattgccggg cgaacaaaat gaaaagaggc ccgttgccgg gcctctttta
    49261 ttgattccaa tcaaggatag ttacttaacc acgacaaact tgcggctgaa cgtatcgcca
    49321 ttggccttta caaa
//
LOCUS       DAOPYV010000014        47031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_617_length_47031_cov_11.675792, whole genome shotgun sequence.
ACCESSION   DAOPYV010000014 DAOPYV010000000
VERSION     DAOPYV010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 47031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47031
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_617_length_47031_cov_11.675792"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(523..1233)
                     /locus_tag="PKI35_03080"
     CDS             complement(523..1233)
                     /locus_tag="PKI35_03080"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX43395.1"
                     /translation="MLESINPENVLFIDIETVPAQPAFDQLSGRLQKLWERKADRLKS
                     NESDTPVSLYARAGIYSEFGKIICISAGMFRQNKFRIKTYANDDEAVLLSGFSSLLNQ
                     HFYKQEHLLCAHNGREFDFPYIARRLMVNGMRIPSILDNSGKKPWEIGLLDTMDLWKF
                     GDYKNYTSLELLAAIFDIPTPKDDIDGSMVADVYWNQHDLPRIAEYCAKDVLTIAQLF
                     RRFRYEPLLTPDEIVIVA"
     gene            1344..2645
                     /locus_tag="PKI35_03085"
     CDS             1344..2645
                     /locus_tag="PKI35_03085"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNX43396.1"
                     /translation="MLIKAIRWIIILLIAAAAIQSCKEDPDESWPEVNIISPSSGSDF
                     RVYDTIKVEATFSDDRALESVKVALVGENNTPLTTPVSAVPNSNPYTFRAELIIDDAS
                     LTGGTYKLLFQAFDGTNTANTYLTIYLEALPEALLYPVVVTAAGSNQIRIMRTDTNDV
                     FQSLYLYQGDYGGSDVSSAFGQLYFSGKAKADMKAYSLLTNKFLWSVPYEQSMTGHWF
                     EGVQCFYPWVLVSHYDGLIRGYDRNGIQQFTSQPVSNYYPGPAIMISPYIVVSMKEYN
                     SPLYQLAAFHAAGGKLLDLKPVSFTPVGFEPAGAQKVFVFGNLDGDGVIALYDLMNSN
                     LSILKTIMDHSISDVDIMSDNVFYFAAGDEIYCYTYQNNSVIPFAVGYENARISCEKL
                     NGQVYAASGNSLYVLNTPDGSVEKSYLFNDPVLNVHLVYNK"
     gene            3196..3873
                     /gene="mnmD"
                     /locus_tag="PKI35_03090"
     CDS             3196..3873
                     /gene="mnmD"
                     /locus_tag="PKI35_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HNX43397.1"
                     /translation="MKVQIEETADGSQTLYVPELNEHYHSIRGAVSESRHVFLSAGFD
                     HCRTAERKINLLEVGFGTGLNALLTCMEAQNKDIQVNYIGIEAYPLTDEVIAGLNYAG
                     IIGAPGADELFRKIHEANWIYPSYITDRFLLSKIEAKLEDVSLAANMFQLIYFDAFAP
                     DVQPELWSEEIFRKLFACLVPSGILVTYSSKGTVKQALRNAGFVVERLPGAMGKHHML
                     RAVKPLL"
     gene            complement(4111..4377)
                     /locus_tag="PKI35_03095"
     CDS             complement(4111..4377)
                     /locus_tag="PKI35_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNX43398.1"
                     /translation="MRSIEITIRGRVQGVGFRYYTLRLANELQITGYVRNKPDGSVFI
                     EAEGDNMNIQAFLSWCRQGPPRAYVEEVIVNDCPPAGYLRFDVR"
     gene            complement(4529..4723)
                     /locus_tag="PKI35_03100"
     CDS             complement(4529..4723)
                     /locus_tag="PKI35_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43399.1"
                     /translation="MSSEFQTFIHDTTGMIGMKDKLKLWNKMYDEYSLDKKLISLDIT
                     EFTSEVEKFRSELLQKLRNY"
     gene            complement(5192..5917)
                     /gene="bla"
                     /locus_tag="PKI35_03105"
     CDS             complement(5192..5917)
                     /gene="bla"
                     /locus_tag="PKI35_03105"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015083750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase"
                     /protein_id="HNX43400.1"
                     /translation="MIFILAGWIQVTGFSQPRYQSVSISEDLELIRISENAYIHVSYA
                     EVPGMGRVSANGLIFISGHEAFLFDTPWDDSLTKELYDWITGPMGLEIAGFVPNHWHK
                     DCLGGLGFLQSRNVESYANRMTIGLAKLNNLPVPGHGFTDSLLLKCGDQQIECYYPGA
                     AHSLDNIVVWIPAEKILFAGCMVKSLDAKNLGNTADGDLDAYPETLEKLIRKFPDAKI
                     VIPGHGLSGGLELILHTIELTRK"
     gene            complement(6294..6800)
                     /locus_tag="PKI35_03110"
     CDS             complement(6294..6800)
                     /locus_tag="PKI35_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43401.1"
                     /translation="MTLVPGLNADFYKLFKGEFIPEHVVECIPSLGSKTFDFLNSGYA
                     GLYMVSKKIIELFNNNAISGWSSIPVRIKGYEGYEYYLFTVTGRCSAIDYEKSEASIK
                     APFTTTGRPVEAKRGLYFDLSSWDGSDVFTPEESMFTFVTEKVEKLLYKNKVTNLIIE
                     NITKFELL"
     gene            complement(7026..7433)
                     /locus_tag="PKI35_03115"
     CDS             complement(7026..7433)
                     /locus_tag="PKI35_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43402.1"
                     /translation="MNTINFYDKERNLIIQLKDFQFVKTEALKIVTFKGKVDFDFFHI
                     ETNFEAEEYDFTNLQLSLEKMYNGEQNYISFNPFSGKLTLKLSEEGSQIKIKGEVYND
                     LSTCKLEFKYNIDQSFIPELIKELKEMMMREDM"
     gene            complement(7950..10463)
                     /locus_tag="PKI35_03120"
     CDS             complement(7950..10463)
                     /locus_tag="PKI35_03120"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HNX43403.1"
                     /translation="MKNRNTIFRTLLFILLILTARMAEAQTNQYLDFDGIDDYVVTPN
                     GESYITNSTQGITMAGWFYTNALVYGQGMMGFRGGATEFYVIQLSDGKLECRYKSSTG
                     TLYEYAAPANTIIPQVWQHIAWVFDGSAVKLYINGNLKGSKAAAGVLTDGTVPFAIGK
                     SLLGGYNFIFGGRADEVSVWKRGLTQAEIQEMMANELTGTEADLQMYYKFNQGVPGGD
                     NTSITQLITQVYAPERNGDLLNFALTGPTSNFNGTLDSSYQAISFPQIPDKLNSTAPF
                     AISATSTSGLEVQFNILSGPATLNGNIVTLTGDTGMVFIEASQPGNSQYNPADPVINS
                     FMVLDPALHIPEIELRNPLAGNVYVPSLSQIQLAAISKITYPELFHVTGVQFRIDGQT
                     IQAQDFYNTHYTAWWTPPAYGTYSLQVLATNNYGATATKTYSINIQQDVEDMEVQAAD
                     DIWLSSVKVSQEVEAELPSFLGAFDKISAVLTVNCPSGGCGEYDRVAWVEAQGHDGRW
                     IEIIRYITPFGKACTHSIDLTDYMSVLQGKIRFRINCTTLDNGYLYDLSFLYRAGVPT
                     HKYSYITEVWNQIFPFGDYANLQPVDTFYYSYPANAVASKLKLISTGHGWGNLNTGNA
                     AEFYKATHHIYVNGDNTFSQLNWCDCTPNPDSCENQQGTYKYDRAGWCPGSIAKWFDY
                     DMTPYVSGGDISLMYKFYEQYMDNCHPNNPDCVTGVTCSDCSDGFNPTLDVACNLVSY
                     SDSPIVIVGKDEPINYFPGAIKVVPNPTDGKFELSATSEKAYRNSTLFIYNNTGKLVM
                     QSSWNGETTTFDLSDQPQGLYLMKVIKPHGRESVKIIKN"
     gene            complement(10503..12122)
                     /locus_tag="PKI35_03125"
     CDS             complement(10503..12122)
                     /locus_tag="PKI35_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNX43404.1"
                     /translation="MKKYIPALLIIPALLFIGSCTKDFSEINTNPLGFTTASDGALFN
                     GIIESLVLSGNEQFYINNEILYKQTQLAALTKDAWGNYTIGTEALWTNYYEILPSFRE
                     LEKRFNTAEPSKSVNNMKAMLDIVFAYKTFKMTDLFGDMPVSKAGYGFQSLEYLHPEY
                     DTQRDIYLSMLEKLRVADSLICDTTAEVEPFTSFKTFDKLFKGNLTMWQKFANSLRLR
                     YAMRMVNQEPEIAGNIIKKIIEDGRPVLLGYDFVNPVLESACLWPLEIGYKNESLNWS
                     FREHNGLRMGSNIWHQFSSTDSPSGSGIFDPRAYIFFETDVNNQWTPYPQIPDNTTPS
                     SGGLPYESYRDDAANFTLKQNCNYSPFNYFLIRDQDYMPIILMTGAEVHFIKAEAYLR
                     GIGVTQDRDMADIEYLNGILSSVKWWLNVAASSQLPISGVKFEDMITIPQNVNSGSVL
                     THFGSWNASNDEEFLGLIYTQCWIDMFRQPWDAYALTRRTGMTPREGDPINHFRMPYP
                     PSEYEYNTVNCNDAISRQGGDSPQTKIWWAK"
     gene            complement(12170..15490)
                     /locus_tag="PKI35_03130"
     CDS             complement(12170..15490)
                     /locus_tag="PKI35_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX43405.1"
                     /translation="MGKSTQLLLLFLTFSLLTSAQQMAVTGTVTSSEDGSTLPGVSIL
                     IKGTSTGTTTDINGKYALSRLSSSNILVFSFLGYEKQEITVRNQSVINVVMKPAATRL
                     EEVVVTALGVKRQKREIGYSTEKIETKQIANAGAPNIINALAGRSAGVMVSQGDGVDG
                     GTTRITIRGNNNLGGNNEPLIVVDNVPLENTEGLTDIGRGVDWGNGISDLNSLDIEEY
                     SVLKGGAASALYGSRGANGVILITTKRGKKQSGLGITYNFEEKITHPYRYREVQNKYG
                     AGGPVSFTEPAFPVDENGILQYPGVYGNDKLVINQEGKTSSTSEEFGYYGSSVSWGPE
                     MKGQLVKWWDGEMRNYSPQPDNFKEPYQDGFTRTHNIEATGGSEKGTLRISLTRQDHK
                     PIIRNSNYDRTTINVGTTINISEKVKADAVLTYVRFNRLNSPILGEDQNSFNKGYLYS
                     WPRSYQNLDLKNYQLADGSQNKLDGYPFLYVDRYLWWDYYNNNTQLSRDKYTGSLALS
                     YAVTPWLNFTGRAGRDYVVESKTATSKPTDVIGLLGGSYSNELSKCYNDNFDLILTAE
                     KKMIFNSPVTVKFTAGANRWDYNYYSIYGHSGTWYYPNMYTFFNYTETNYVKDENGQI
                     MVEETGDALSKVATQEGISRIRNNSVYSFLNFAYKDYLFLEFTARNDWSSTLPSYSNS
                     FIYPSVSASYIASEALQLQKHLPWLNFLKIRGGISQTASGGNPYQAHFYYTTSFFGGD
                     QTSYFPSRIPPYRLKPQRVNSYEAGVNIGLFENRIDIDFTWYYKYSFDQILDLPVPVS
                     SGAPGISINEGTLSNRGVELIINTVPVQTNNLIIKAGFNFARNRNKIVSLGDYSDTYL
                     LAEIWGLNGPAIALKEGDDYGTIVGYDYLYNDKGERILNDEGTKFLITDNRVPVGNAS
                     PDFTGAVNADISWKGLRLSALVDTKWGGDIYCGSYVISLQTGQSPSTLLERDGGGLPY
                     TDPEGNVRNVGVILDGVYADGTKNTKVVHYYYKYLPNAGGWGKFISKPGILENTWVKM
                     REISLSYRIPESLTKKIKVFQALTINLTGRDLFYIYTTLPDKINPEGIMGSGNAQGFE
                     WASYPSTQSFSFGVNASF"
     gene            complement(15981..16550)
                     /locus_tag="PKI35_03135"
     CDS             complement(15981..16550)
                     /locus_tag="PKI35_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43406.1"
                     /translation="MHDIEPFYSWRLLYISSEDERSPFYGREYSEFEFTHQIYNYLIH
                     PQWDEFGSATLYLKILYADYVKNFCIIELFGEWNDCLYNDIMYLKRNVIEYLIEQGIT
                     KFIFIGENVLEFHASDDSYYQEWFEEIDDGWIVLLNFREHVIREIEQSNIDYYLAVGG
                     KFNNIPWRVHNPVKLFSIVEQMMTRRLKE"
     gene            complement(16593..17816)
                     /locus_tag="PKI35_03140"
     CDS             complement(16593..17816)
                     /locus_tag="PKI35_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNX43407.1"
                     /translation="MDLSNNNLQNLLHGAAYMPREETLEIKKTPRQLTIGIPRETSLN
                     ENRIALVPDAVGLLVQQGHRVIIENKAGESAHFSNHDYSEMGGEIVPDVRQVYTADIV
                     VKIAPPDCSETDMLTSRQAIISSLYHPGLTEEYFRKLTAKKSTALAFEFLQDKTGTFP
                     IIRAMSEIAGNTSILLAAEFMSHAEYGRGKMLGGFSGITPSEVVILGAGTVGEFAARA
                     ALGMGAFVKIFDNSIYKLRRLQNNLNVRIFTSILQPRILLNNLKTADVVIGAVHSTVG
                     RTPCLVTEEMVQQMKEGAVLIDVSIDQGGCFETSHPTDHQNPVFRKYGVTHYCVPNIP
                     SKVPHTASYALSNILTPILEKIGEEGGLEQVLHSNYGVRQSVYIFNGISTNKFISDTY
                     RLPFQDINLLMAAFH"
     gene            complement(17830..18249)
                     /gene="tsaE"
                     /locus_tag="PKI35_03145"
     CDS             complement(17830..18249)
                     /gene="tsaE"
                     /locus_tag="PKI35_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNX43408.1"
                     /translation="MPQTYTCKSPGELADIAKSLLSAWPGRRIFAFYGNMGAGKTTFI
                     KEFCRQLGVETIVNSPTFAIVNEYLAKNGQSIYHFDLYRLKNWEEMLDIGYEDYFFSG
                     NYCLIEWPEKIENLLPSDAVEVRIETDAETEARYFTF"
     gene            complement(18257..19810)
                     /locus_tag="PKI35_03150"
     CDS             complement(18257..19810)
                     /locus_tag="PKI35_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HNX43409.1"
                     /translation="MENGVILWADDEIDLLKPHILFLREKGYQVYTTNNGDEAIDLLK
                     SQPVDIVFLDEQMPGLSGIETLSIIKASHPNLPVVMITKSEEEHIMEDAIGSNISDYL
                     IKPVNPKQILLSVKKNLENKRLISEKTTYAYQQQFRNIGMEFSGRLSHEEWGEVYKKL
                     VYWELELEKSQDQGIVDVMRMQKEEANQVFCKFVENNYVDWVNGKAVDKPVQSHTLLR
                     DKVFPLINTDQNLFMILIDNMRYDQWKVLQPIIEQYYRVERDEVYYAIVPTTTQYARN
                     SLFAGLLPSEIEKKYPKYWVDEDEEGTKNQFENELLGENLKRQAKNVAYSYTKVLNLA
                     SGQKLAETLPNLMGNKFNVIVYNFVDMLSHARTEMEVIRELADDEAAYRSLTVSWFAH
                     SPLFDIIRFLAEKKVNLIITTDHGSVRVSNPVKIIGDRNTNTNLRYKTGRSLNYDKRE
                     VFEIKNPADIFLPRSNVSSAYVFSRRDDFFAYPNNYNYYVNYYRNTFQHGGISMEEMI
                     VPIAFLKAK"
     gene            20011..21264
                     /locus_tag="PKI35_03155"
     CDS             20011..21264
                     /locus_tag="PKI35_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNX43410.1"
                     /translation="MPNKRKIINDPLHGFITVPDELTFDVIEHRYFQRLRRIKQLGFT
                     SMVYPGALHTRFHHALGAMHLMRQAIETLRIKGFRITDDEAAGASLAILLHDVGHGPY
                     SHALENCLVYGMNHEDITVFFLDRLNEEFEGKLETAIRIFKDEYPEKFLHQLISGQLD
                     VDRLDYLMRDSFFTGVSEGVISTDRIIKMLTLVDGEIAVEGKGIYSIEKFVVARRLMY
                     WQVYFHKTVLAAEYTLLNILRRAKYLSERGAQLFATPAFSFFLSNHITADDFRDDRQI
                     LELFSQLDDFDIFSAIKVWTSHEDRILSMLSRALVYRNLPRVELQSGPFDDSFVGELK
                     KRQKAQMQLSDEDLDYFIFTGSIANDAYIPFRDKINIVDKQGHISDIAMASDQLNVAI
                     LSKTVTKYFICYPKSIGIKEGRSNG"
     gene            21356..22405
                     /gene="lpxD"
                     /locus_tag="PKI35_03160"
     CDS             21356..22405
                     /gene="lpxD"
                     /locus_tag="PKI35_03160"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNX43411.1"
                     /translation="MKFKASEIAALIGGAVEGDPEIVITKIAKIEDGESQAISFLANP
                     KYEPFIYNTQSSVVIVKTDFRPEHPLSCTLIKVDDPYTAIAVLLDMYNKMNKPRTGIS
                     SLAFISGSAKTGNDVFIGEFAFVGENAVIENEVKIYPQAYIGDNVKIGEGTIIYPGVV
                     IYHDCEIGKHCTVHAGVVLGADGFGFAPQDDNNYMKIAQIGNVVIEDNVEIGANTTID
                     RATMGSTIIRKGVKLDNLIQIGHNVEIGENTVVAAQAGFAGSTHLGRNCMIGGQAGFA
                     GHIVIADDVKIGAQAGVPNNIPKQGSIMLGAPAIDIRQFRRSVAVFNHLDKLAQRVSE
                     LEKKLAEATNQPEGK"
     gene            22447..23865
                     /locus_tag="PKI35_03165"
     CDS             22447..23865
                     /locus_tag="PKI35_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HNX43412.1"
                     /translation="MVEKQCTIKQPVSLSGAGLHSGKDVTLTFRPAEAGHGIKFRRID
                     MDGQPVIDAIADYVVDTSRGTTIQHQGVRVATIEHTLAAVVGMGIDNILIDVDSPEMP
                     IMDGSAAAFAEALSKAGIVEQDADREYFEAHTTLTYSDPHRKSEMILVPEDNFRVSTM
                     IDFETRILGTQYASIDDISNFRDEIANCRTFVFLHELEYLISNNLIKGGDINNAIVFI
                     DRLLGDAELDRLAKYFNKDKIEVLKEGILNNLELRHHNEPARHKLLDVIGDLALIGMR
                     IKGHVIATRPGHAVNTSFAKLVRDQVILSKQKPTFDIFKTPVYDINDIRRFLPHRPPF
                     LLIDKIIEISDNHVVGVKNVTMNEPHFTGHFPEEPVMPGVLQLEAMAQTGGILALHNV
                     PDPENYATYFLKIDNVKFRHKVVPGDTLLLSLELISPVRRGIFHTKANAFVNNVLVTE
                     AELVAQITRKKTVNTDNNTVKS"
     gene            23862..24650
                     /gene="lpxA"
                     /locus_tag="PKI35_03170"
     CDS             23862..24650
                     /gene="lpxA"
                     /locus_tag="PKI35_03170"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNX43413.1"
                     /translation="MNQPLAYVHPQAKIAKNVVIEPFVNIEKNVEIGEGTWIGSNVTI
                     MEGATIGKNCRIFPGAVIAAIPQDLKYNGEETVVRIGDNTTIREFVTINRGTKASWET
                     VIGNNCLIMAYVHIAHDCSVGNNCVLANSVALAGHISVEDWAVIGGHSAVHQFVSIGQ
                     HSMTSGGALVRKDVPPYTKAAREPISFVGINSIGLRRRGFTSEKIAEIQEIYRILYLK
                     GYNVTQAVGIIETSMPPTSERDEIISFIARSQRGIMRGFSSGRE"
     gene            24717..25346
                     /locus_tag="PKI35_03175"
     CDS             24717..25346
                     /locus_tag="PKI35_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX43414.1"
                     /translation="MEIFLDNISRKYNHEWVFRNLSFTFSSGNSCAVLGPNGSGKSTL
                     LQIISGHLHPTGGTVQYRLNGSMLPDEQIFRYIAITAPYIELIEEFTLPEMISFHLKF
                     RNLRHGMDVKEFMDILDIGSNRDKCLKYFSSGMKQRVKLALAILSDVPVLLLDEPATN
                     LDASGVAWYRGLVAEHCADRIAVICSNSQPAECDFCHTSLLVENYKGTR"
     gene            25487..26050
                     /gene="efp"
                     /locus_tag="PKI35_03180"
     CDS             25487..26050
                     /gene="efp"
                     /locus_tag="PKI35_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNX43415.1"
                     /translation="MATTADFRNGLCIEFKGDLYKIVEFQHVKPGKGAAYVRTKLKNL
                     KSGKVLDNTFDAGERIDVARIERRPYQFLYKDDSGYHFMNTQTFEQTFVEEEMVDGHQ
                     FLKEGQEVEIMFHADTESILSCELPSIVELEITYTEPGLRGDTATNTLKPATVETGAT
                     VRVPLFIEAGTRIRIDSRTGDYIERAK"
     gene            26097..27026
                     /locus_tag="PKI35_03185"
     CDS             26097..27026
                     /locus_tag="PKI35_03185"
                     /inference="COORDINATES: protein motif:HMM:NF016494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HNX43416.1"
                     /translation="MKFALPLTLQQIAAFLQAEFSGDPGFPVKGLNEIHMVEAGDLTF
                     VDHPKYYNKALGSKATTIIINKKVDCPKGKSLIFSEDPFACYVKLAKFYSPFVPASSM
                     LSDSAVIGGGTVIQPGAFVGNHVVIGKNCIIHPGVSIYDGTRIGDNVIIHANSVLGSD
                     AYYFQRKPEGYRKLVSCGRVIIGNNVEIGALCSIDKGVSGDTIISDGVKFDNHVQVGH
                     DTFIGKNCLIGAHTAIAGVTRIEDDVIIWARVSVNKDIVIGKGAVILATSGVDKSLEG
                     GRTYYGVPADEAFKKWREVAAIRKLPGLFRKLQ"
     gene            complement(27071..28558)
                     /locus_tag="PKI35_03190"
     CDS             complement(27071..28558)
                     /locus_tag="PKI35_03190"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNX43417.1"
                     /translation="MTDRIKISIVYIITALFILLNTYLVVKKEYYWGIFIPLAIMLIL
                     LYVFHFEKAFYLAVLSTPLAVNVGNYDLGISISLPSEPLLIGILLVFALKVIIEGYPD
                     KKILRHPVSIVIFISLIWMFITSCTSVRPIVSFKYFAARLWFVIPMFYLGAFLFRDVK
                     KARSFGWAYVIPLIIVIAITTFEHKQYGFSEKAGHWVMQPFYNDHTAYGAILSLFIPL
                     LVGYIFDKSSSVRIRIFASIAAAILFMALYLSFSRAAWVSCALAAAILILIVLRIKFK
                     WIAITVVGIFAIFFTFKFEILDKLEKNKQDSSANVIEHIQSISNISSDASNLERINRW
                     QSALRMYHERPLFGWGPGTYQFEYAPFQSSREKTIISTNLGDLGNAHSEYIGPLAETG
                     FPGLLIVLAIMITAIYTGLKVYRDAPDRRVRLLSLSVTLGLITYYIHGIMNNFLDTDK
                     ASVPFWGFVAILVALDVYHCRNAKNTDDKQSSSGAPQLQEPSSGE"
     gene            complement(28604..29524)
                     /locus_tag="PKI35_03195"
     CDS             complement(28604..29524)
                     /locus_tag="PKI35_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43418.1"
                     /translation="MENFFDNNNLLKILAKWKWHLLILSILAALVSLIVSSPLVITPR
                     FKSVAVAYPSNIAPYSDESETEQMLQWINSKDVKDSVMRKFDLPKHYGIDSSYEYFTS
                     TMSYLYDKFVKVAKTPYESVEITVFDRDPVVARDMVNAIIYYTNMKIRDAHRQKYIEV
                     VDGYKSALAVKQREIDSTKSLLNAHQGNNDFLSKSAETMGVINDNRKPSPVRTGIKNA
                     DGDLLYLAYRMYGLANEYDLIMQEYDLAVFDTSKEFTFLNVVTPPQIADKKSYPKRVL
                     IMLYFVGGALLLSLLTISIIEKKKSKPTEQ"
     gene            complement(29580..30644)
                     /locus_tag="PKI35_03200"
     CDS             complement(29580..30644)
                     /locus_tag="PKI35_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43419.1"
                     /translation="MSVSTPSTDTKPPSVIGFILKWRKVFIITSVAAALLSLIFSSPV
                     FITPRYRSTVIMYPASSNSISKALLNDNSNVDKDILEFGEDEQTEQMLQVLNSGLLRD
                     KIISKFRLADHYGIKPSSRFFNTRLYKQYENNITFKRTEYMAVKVSVLDRDPQMAADI
                     ANSIASLFDTVKNDMQKERAMQGYRIVEEEYNRLQQEVRKKEDSLTDIRKKGVHDYET
                     QAEMINQQLAVEIARGNKTGVKALEKKLDILSQYGGAYVSLRDALELEQEQLNFVKTR
                     YDEAKIDAHKSIPQKFIVQNAYKAEKKAYPVRWLIVLTSILSTFLITAMAIAAVEQWG
                     NELQKLKSFPVKKLREPSEK"
     gene            complement(30768..32210)
                     /locus_tag="PKI35_03205"
     CDS             complement(30768..32210)
                     /locus_tag="PKI35_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNX43420.1"
                     /translation="MRRKFLTNLGLLLFLNFLVKPFWILGIDRTVQNITGPEEYGFYA
                     SLLNFTFIFNILLDFGITNFNNRNIAQNSQLLNKHFSGIVSFKLLLGILFFVVTLGVG
                     LIIGYKPRQMQFLMILGFNQFLTSFILYLRSNISGILHFKTDSVISVLDRLIMILICS
                     VLLWGRVTDQPFRIEWFVYAQTIAYLITAVIAFGIVVRKSAFKKLQLSIPFARMIIKQ
                     SFPYAVLVLLMASYNRIDSVMLERLLPDNGDMQAGIYASAYRLLDAVNMIAYLFSVIL
                     LPMFAKLIKEKLSVEWLVKLSFTLLFTASIIVSAGSYFYSFELMDLLYVSHVTESSNI
                     FRLLIIGFIPIATTYIFGTLLTANGSMKALNITAFLGMIINIGLNFWLIPKYFAIGSA
                     YASLVTQFVTGIIQIIIVQRQFKFHINKKFLITLPVYVAGVIAINIGTKSLSLAWETR
                     FLMMMAFSLLLAIALKLFNFRSLFELIRNR"
     gene            32425..33549
                     /locus_tag="PKI35_03210"
     CDS             32425..33549
                     /locus_tag="PKI35_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNX43421.1"
                     /translation="MKIAVNTRLLISNRIEGIGRFSVETLSRMARAHPEHEFCFIFDR
                     PYDKAFVFSDNVYPYVIPPQARHPFLFLMWFELSVPLLFRRIKPDLFVSPDGYLSLNT
                     RVPSVVVMHDLNFEHFPQDLPWLISRYYRSMFPRFARKAKRIATVSEYSKQDIAERYS
                     ISHEKIDVVYNGVSSHFHPLSEAEKETVRRSFSSNKPYFLFVGALNPRKNLKNLFLAF
                     DKFKETDDLEIKLIVAGARMWWNDDMRFTYENMHHRHDVIFTGRVTDAELVRLVGSAL
                     SLTYVSGFEGFGIPILEAFQCKTPVITSNVTSMPEVAGDAALLVDPFSVEEISEAMVR
                     MAREPELRRQLIEKGTEQCTKFSWDRSAELLWNCIEKTIR"
     gene            33585..34280
                     /locus_tag="PKI35_03215"
     CDS             33585..34280
                     /locus_tag="PKI35_03215"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNX43422.1"
                     /translation="MKYSPIALTKNQQYDRQTKRVIRKVCQHDTNTVDVGCHKGEILD
                     ILLEHAPDGVHYGFEPIPGLFHSLEAKYSGFANCHILDFALSNVKGSSSFNLVLDDPA
                     LSGLKKRAYNPGYRSQTIEVKTERLDDIIPATTKVGLIKIDVEGGELQVLMGASELIC
                     RSKPVIIFEHGQGASEFYGTRPEHVFDLLEKCGLKVSLMSRWLKQGPALPKAGFIEQY
                     QKKVNYYFIAYPD"
     gene            complement(34298..35317)
                     /gene="murB"
                     /locus_tag="PKI35_03220"
     CDS             complement(34298..35317)
                     /gene="murB"
                     /locus_tag="PKI35_03220"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNX43423.1"
                     /translation="MLNILENVGLKPYTTFGIEATARQFVEIKNQEEAVQLVESGVLR
                     DKLHLVIGGGSNILFTGDFDGLVIHVANRGIRMVESAPDSALVEAAAGEVWHDLVSWA
                     IKQGLGGLENLSLVPGNVGAGPVQNIGAYGVELKDYFHSLRAIDLETGSEKVFTKDEC
                     RFGYRDSIFKRALKGKMLILAVTFCLSKTPVLRLDYGAIRQELEAMNIKEPAVADVSE
                     AICRIRRNKLPDPAVTGNAGSFFKNPVVSSGKAETLIAGYPGIPVYHQPDGSMKLAAG
                     WLIETCGWKGYREGDAGVHPKQALVLVNYGNATGIDIITLAGKIQDSVLDKFGVKLEM
                     EVNIV"
     gene            35438..36559
                     /locus_tag="PKI35_03225"
     CDS             35438..36559
                     /locus_tag="PKI35_03225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX43424.1"
                     /translation="MSSKKAIISVINDLVTDQRVDKTARVLLELGFEVIMVGRRKRDS
                     MPMPLKPYKTKRMKLLWEKGPLFYAEYNTRLFLYLLTHRASLLMSNDLDTLLPNFLVG
                     RFFRAPLVYDSHEYFTETPELTGRPLVQSVWKFIERMIVPKLKDCITVNESIAGLFYN
                     LYGIKFKVVRNIPAKKNHASVLSREDLGLPVDKKIILLQGAGINIQRGAEEAVEAMRY
                     LDGAILVIVGGGDVLPLLKKQVAESGLQSSVMFVPRQVPEKLFSYTVNADIGLAIDKD
                     TNINYRFSLPNKLFDYIQAGIPVLVSPLPEIKRIVDEYNIGDFIESHDPQHIAGKMSA
                     MLSDQDRINTWKTNLKVAAEKLNWENEKQVLTEIYSQYA"
     gene            36552..37691
                     /locus_tag="PKI35_03230"
     CDS             36552..37691
                     /locus_tag="PKI35_03230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX43425.1"
                     /translation="MPDRHLNIVSFAIPYPPNYGGVIDVYHKLVALKNLGVKVHLHCF
                     RYDRQPAPELENICESIHYYPRKTGFFSALRGKPYIVISRRSEELLKNLCLNDYPVLF
                     EGLHTCLYLPHEALRGRSLIYRESNIEHDYYRHLANAEKNPFVKAFMYIEAFKLASFQ
                     RVLKHASAMLAVSQSDASYLHSRFPSGKVIYLPSFHGNYTVKSKEGRGAYALYHGNLT
                     VAENVKAAEYLIKEVFNNLDIPLKVAGLNPSGSLKKLADKYGNVSLIASPDEKRMNEL
                     VSEAHVNILVTFQPTGLKLKLLNTLYKGRFVLVNPDMLAGSGLDVLCETANDTISLKA
                     SLLQLFTETFDAGKIIQRRDYLQKHYSDEANAKKLVEVVFGGDQD"
     gene            complement(37868..38440)
                     /locus_tag="PKI35_03235"
     CDS             complement(37868..38440)
                     /locus_tag="PKI35_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43426.1"
                     /translation="MKNLIYLLAVALVMLFSCTKAPMDEEVSPGVKNLKKVPVPMKID
                     ICMTPDTNRLHVEGTPLVNELGDTIIPSLDLSVGSLLSGHGTHIGQLIKEESNMTGYD
                     AYLMTELLPQGKVVIAATYDGILTAANHDKLSYISYIQIDVTIPPTKIITGDVELTGG
                     TGRFEGATGNCTINGIVPCWSVDGYILFAK"
     gene            complement(38650..40851)
                     /locus_tag="PKI35_03240"
     CDS             complement(38650..40851)
                     /locus_tag="PKI35_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX43427.1"
                     /translation="MNPVIERLQKIESLMLTRLSYPGCTSKQLQGNLSAMKNIFFTFT
                     YILILVSGCLIFAPELSIIIGYGLTLMTLLALSFSLQLIFPRHFLFIGLSHGLALNLA
                     TFYCILKMGGIPASGGLIFVGIANVLSTVPRQKAWYSITLFSIYLVLVVLLVVLKPWL
                     YPSQLIKPFENTVLYMLNAIFLTGASLIFVLDFIRQQRKLDVIETNHLKEIHEMKNRL
                     YTNITHEFRTPLTVIQGMTDLIRTQPEQWMETGLLKIRANSDILLHLVNQMLCMARIE
                     TGAVTISLCRKDIINYLYSLVEQFKPEAITRKIDLTFEPEEASFNMDFDEKKLMHIMS
                     NLISNALKFTAEGGRVKVEANGHEDAEQFAIRVKDTGIGIRKEDMDHLFDRFYQVENN
                     NSPGGSGLGLAVVKDAVELLKGTISVTSIPGIGTEFSVFLPVTRNAPETDIRQEADET
                     EETRQQANGDWSASLIFRDASPEVVQEEAVMQDGAPLLLIVEDNLDLVEYLRAILQKK
                     YHVEVAKNGSTGFEKALNLVPEIILSDVMMPVLDGIAMLEKLKNDIRTSHIPVVMLTA
                     KADIDSRLAGLERGADAYLAKPFDEKELFIQLKNLVDQRKKLHERYSSIEKIPATSDI
                     YLKKEDEFMIRVRQVLEANLENEEFGITQLCNELAVSRGQLYQKFKSLSNKTIADYFK
                     FLRLQKAKELLLTTSLNVTEVTFAVGFKNLSHFSREFTRQFGKSPGKFRKP"
     gene            41181..42854
                     /locus_tag="PKI35_03245"
     CDS             41181..42854
                     /locus_tag="PKI35_03245"
                     /inference="COORDINATES: protein motif:HMM:NF016611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX43428.1"
                     /translation="MKKFVFLLIFIPAFAFGQKPAATATAAGKDLKESAEAFVTRFIQ
                     CWDDKNWDQIFNSIEETGTYYSAAETTPLPEMAKSTVNYYKYKVTDARTTVNFVSADV
                     YGQNTAMVTVRYLESLKAEGRDRVLDYLDILVLEMKEGNWKISRWYSQCFYPVIVNPA
                     IDGKWQKNVAEPVWRYSGALYQFYNLNCYLIEDNKKNGTSPAQLGKWMGERFAKTWNQ
                     SKGFEGLVSGYFWLMQSISPYVEFTERNENIARIKFKFYNPDPEDWNVTENEMLECFR
                     NVLIEIAANMGGTCSIAPDGKFYMMTLQAVPGWLPDKDSKIVKESLARGDTVKADLVK
                     ANFLTSQGKTEEASKIFTRIMKIRPHNKEAVQGWLLANMKKSPTGEEDAIKSLEELEK
                     LYPENSGVIFWKSFIEAEYGHNEEALKDCNRLIKIQPDSAINYIGKGQVLYAMERYKE
                     AFKAFDKATSLNPERSDVWGMKGAALARLGRYDEAIASMNRGLKQDPDNPNNIYNRAC
                     IYSLKGDKARALADLKTAISLNPEFREYSRKDKDFERLYDDEDFKKLTE"
     gene            complement(42974..44044)
                     /locus_tag="PKI35_03250"
     CDS             complement(42974..44044)
                     /locus_tag="PKI35_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNX43429.1"
                     /translation="MKRPLFLLLLLFALVIPGTAQNDDSTLKEINAKIESLGYDIASL
                     QKAVDDIYWYNRVGDVAFIDKVYITGPPVPEKQVKNPTAMGARNPVKFYSYIFIPKDI
                     KADKKYPLLVLPHGGVHADFTTYHAHIIRELMAQQYIVIAPEYRGSTGYGKDFYEKID
                     YGGLENDDVNASRDYMVRNYEMVDSTRVGIIGWSHGGMIALMCVFDHPEKYQVCFAGV
                     PVSDLVARMGYSDDEYRKLFSATYHIGKDAREDIKEYRRRSPVWNAWKLQTPLLIHTN
                     TNDDDVNVLEVEHLIQALKAEGKHFEYEIFQDVEGGHSFDRIDTRIAKQIRLKIYAFL
                     DKYLDPPHKLKSLSDIDKAAYR"
     gene            complement(44375..45055)
                     /locus_tag="PKI35_03255"
     CDS             complement(44375..45055)
                     /locus_tag="PKI35_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HNX43430.1"
                     /translation="MKTKAFLLLTTSLTLGVIVALFSSCNKDEDSGSSTIKDIDGNVY
                     HSVTIGTQVWMVENLKVTKYNDNTSIDNITNTNTWNSLTSGAYCNYNNLDSYVSTYGR
                     LYNWQAVNTGKLAPKGWRIPTKSDWETLIAFLGGEGVAGGKLKATGSVWASPNTGATN
                     ESGFSGLPGGCNVNGYVEIGLDGNYWSSTSETCNFAWFLNLYKDKSSAELACTNKNYG
                     YSVRCIKE"
     gene            complement(45432..45740)
                     /locus_tag="PKI35_03260"
     CDS             complement(45432..45740)
                     /locus_tag="PKI35_03260"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX43431.1"
                     /translation="MIELNQVGTITSCNPNDNLTLSKITAYPNPSSDYVKVEFRNAKR
                     EKRRLSVYDLLGNEVKVIESTDDDIYIFKTEIGTGVYFFHLQSAADKSERGMGKFVFQ
                     "
     gene            46011..46901
                     /locus_tag="PKI35_03265"
     CDS             46011..46901
                     /locus_tag="PKI35_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43432.1"
                     /translation="MSKFITGKQLEDAVYDIIWNASNSLLIVSPYINLDIYFRKLFEK
                     HLNNPRLQITIVFGKNENNVKRSLSAEDFDFFKKFLNVSVVYSPQLHGKYYGNETQGV
                     ITSINLYDFSFKNNVEFGVFHETNLLNSITKSVDQEAWLACWEIANNAEAVFIKRPVF
                     EKKLISILGKNYVKSDILLDNTERYYKSSLKYKSLPVKRLSDYPDELELGSLPDTRPS
                     REEVESFPRSVSIRDSKPKMGYCIRTGQQIPFDPSHPFCYEAYQEWAKWNNPNYPEKF
                     CHRTGKPSNGKTSKSNPVLY"
BASE COUNT        12638 a        10450 c        11003 g        12940 t
ORIGIN      
        1 gagaacggaa agcagcggac ggacacactc aaaataaaga acgcagaatg aaaaactcca
       61 aaactccaaa actccgggca tttcaagtac tccaagtact ttaggtacta atagcgaaaa
      121 acggagaact ttacctggcg taagtttgct ggtggcaggc aggggaggac ggaggacgga
      181 gaatggagaa cggaaagcag cggacggaca cactcaaaat aaagaacgca gaatgaaaaa
      241 ctccaaaact ccaaaactcc aggcacttca agtacttcaa gtactttagg tactaatagc
      301 gaaaaacgga gaactttacc tggcgcaagt ttgctggtgg caggcagggg aggacggagg
      361 acggagaacg gaaagtagcg gacggacaca ctcaaaataa agaacgcaga atgaaaaact
      421 ccaaaactcc aggcacttca agtacttcaa gtatttaagg cattttaagt accttaggca
      481 ctaataacga aacacgaatc tcacgaatag taatccgccc tgctatgcga ctattactat
      541 ttcatccggc gtcagaagcg gctcatatct gaaacgccgg aatagttggg caatagtaag
      601 tacatccttg gcgcagtatt cagcaatccg cggcagatca tgctggttcc agtaaacatc
      661 ggcaaccata ctgccgtcga tgtcgtcctt tggggttggt atgtcgaaga tggccgccag
      721 caactcgaga gaagtgtagt tcttataatc gccgaatttc cagaggtcca tcgtatccag
      781 caggccaatc tcccagggct ttttacccga attatcgagg attgacggta tcctcattcc
      841 gttgaccatc aatcggcggg cgatgtatgg aaagtcgaat tcccgtccgt tgtgtgcgca
      901 aagcaggtgt tcctgcttat aaaaatgctg gttcagcagg gatgaaaacc cggaaagcaa
      961 tacggcctca tcgtcattcg catatgtctt gatgcggaat ttgttttgcc ggaacatccc
     1021 tgccgaaata cagatgatct ttccgaattc ggaatatatg ccggcccttg cgtaaagtga
     1081 aaccggcgtg tcgctttcat tgcttttgag ccggtcggct tttcgttccc aaagcttttg
     1141 aagccttccg gatagctgat caaatgccgg ctgggcggga actgtttcaa tatcaataaa
     1201 taatacgttt tcaggattaa tagattctaa catggcggta tagtttaagg attaaaatta
     1261 ttaaaatatc tttacccttg ctatagttaa atccggtaat tttaccaacc ttacataatg
     1321 gatagtaatt ttacatttaa actatgctga ttaaagctat acggtggatc atcatcctgc
     1381 tgattgcggc ggcggcaatt caatcctgta aagaggatcc cgacgaatca tggcctgaag
     1441 tgaatattat ttccccttct tccggaagtg atttccgcgt ttacgatacg ataaaagtcg
     1501 aagcaacctt tagcgacgac agggcgcttg aaagtgtgaa agtggcgttg gtaggcgaaa
     1561 acaatacgcc tctgacaact ccggtgtcgg ctgttccgaa ttctaatccc tatacgttca
     1621 gggctgagct gatcatcgac gatgcatccc tgacaggcgg aacttacaag ttgctgtttc
     1681 aggccttcga cgggaccaat accgccaata cttacctgac aatttatctt gaagcattgc
     1741 ctgaagcgtt gctttacccg gtcgttgtaa ccgctgctgg cagcaatcag attaggatca
     1801 tgcgtaccga taccaatgat gtgtttcaaa gcctttacct ctaccagggc gattatggcg
     1861 gatcggacgt cagttcggct ttcgggcagt tgtatttttc ggggaaagca aaagccgata
     1921 tgaaggcata cagcctcctg acaaataaat tcctgtggtc ggtgccgtac gaacaatcga
     1981 tgaccggcca ttggtttgag ggtgttcagt gtttttatcc ctgggtattg gtctcccatt
     2041 atgatggtct gatcaggggc tatgacagga acggaatcca acagtttaca tcacaaccgg
     2101 tcagtaatta ttatccgggc ccggcgatca tgatctcacc gtatatcgta gtttcgatga
     2161 aggaatataa ttcaccgctt tatcagttag ccgcatttca tgcagcgggc ggaaaactgc
     2221 tcgacctgaa gcctgtcagt ttcacacctg taggttttga accggccggc gcccaaaagg
     2281 tatttgtttt cgggaacctg gacggcgacg gagtcattgc actctatgac ctgatgaaca
     2341 gcaatctgtc gattttaaaa accatcatgg atcatagcat cagcgatgtt gatatcatga
     2401 gtgataatgt tttctacttt gccgccggtg atgaaattta ctgttacaca tatcagaaca
     2461 atagcgtcat cccgtttgcc gtcggatacg aaaatgcgcg catttcatgt gaaaaattaa
     2521 acggacaggt atatgccgca tcaggaaata gtttgtatgt gcttaataca ccggacggct
     2581 ccgtcgaaaa atcctattta ttcaatgatc cggttctgaa tgttcacctg gtttataata
     2641 aatagcggag gaagaaatgg tttgtgggga aatggttcaa gagctaattg ttaaaaggca
     2701 gggagaaggg agcagggagc aaggggcagg gagaagggag aacggagcac gggaaagggc
     2761 aaaagtcaaa agtttacctg cctgcctgcg taaggttgtg ggcaagacgg caggcctgcc
     2821 tgccttgcgt gaggctgtgg gcaagacggc agggcaaagg tcaaaagtaa taagtaaaaa
     2881 gtaaaagggc caaggttaaa agccaaaagc caaaaggtaa aagtatcttg cctgtatacg
     2941 gctgtattat aacactggaa ccctgaaacc ctgccccctc ccacagcctt gcgcaaggca
     3001 ggcaggcctg ccgcctcacg cacagcctcc ccgggcaggc aggtctgaaa cgctgaaaca
     3061 ctgaaacgct gaaactctaa gttataggcc aacgaccgac agccagaagg aaaaacactg
     3121 aacgcaaaac acagaactct caactcagaa agccggagcc aacagccaaa tgctggaaaa
     3181 atacgaatga agaatatgaa agttcagata gaagaaaccg cggacgggtc gcaaacgctt
     3241 tacgttcctg aattgaatga acattatcat tccattcggg gggcagtatc cgagtcgcgg
     3301 catgtttttt tatcagccgg attcgatcat tgccggactg ccgaacgcaa gataaattta
     3361 ctggaagtag gatttggtac gggcctgaat gccctgctta cctgcatgga agcacaaaat
     3421 aaggatattc aggttaatta tatcgggatt gaggcttacc cgctgacgga tgaagtaatc
     3481 gccgggttga attatgccgg tataataggg gcgccgggcg ccgatgaact gttcaggaag
     3541 atccacgaag ccaactggat ttacccttcg tatattaccg accggttcct gctcagtaaa
     3601 attgaggcaa aactggaaga tgtttcgctg gctgccaata tgttccaatt aatctatttc
     3661 gacgcttttg caccggatgt tcaaccggaa ctctggagtg aagaaatatt caggaaacta
     3721 tttgcatgcc tcgtccctag cggaatcctg gttacctatt cgagcaaggg aaccgtaaag
     3781 caggcgttac gtaacgcggg atttgtcgtg gagcggcttc ccggtgccat ggggaagcat
     3841 catatgttgc gcgccgttaa gcccctgttg taatgatccc gtttccggca accgggaaac
     3901 taaaaatcag aaagtaataa ctggcgttgt aaaagttgtg agccggaaac tgaaatatca
     3961 aaaccttaac ctgcctgacc ctcgcaggct taagcaaggc agtagccttt caaactctca
     4021 aactttaaaa cagggaaaag gaagcagcct tcagttattc ttcctcatac agcctgattc
     4081 cgtatagcct gaattttaaa ttcgacctct ttaccggaca tcaaacctca ggtatcctgc
     4141 cggcggacaa tcgttgacaa taacttcttc aacgtatgcc cggggcggtc cctgccggca
     4201 ccaggagagg aaagcctgga tattcatgtt atcgccttcc gcttcaataa aaacgctgcc
     4261 atccggttta ttacggacat aaccggttat ctgaagctcg ttggccagcc tcagcgtgta
     4321 atacctgaat ccgacgccct gcacacgccc gcggatggtt atttcgatgc ttctcataga
     4381 tgctttatta caggatgaaa gataatcagg taaaccgggc gattgccata ttcttttgtt
     4441 catgcaaata tacttcagaa gacgaaaatc cggaaatagc cggcgcggtt acctgtccct
     4501 ggttgccgat atttgctgca tacgggtttt agtaattccg gagtttctgt aataattcgg
     4561 acctgaactt ttcaacttca gaggtgaatt cggtaatatc gagactgatc agttttttat
     4621 ccagactgta ttcgtcatac attttgttcc ataatttcaa tttatctttc ataccgatca
     4681 tgccggtggt gtcatgaata aatgtctgga attcgctgct caaaaggaag ctgttattgt
     4741 acagattaaa cgatcggatc catttctctc ctgttgacgg ctttaatttt atccgcatga
     4801 ttttttcggg ccggaaaggt ctggccgtct caaaacagag tcgatttttg ttttcatata
     4861 acctggatat gcggcctttc caggcaggcg gattctgctc attaaaaatt aaggtgtcgg
     4921 tgaaggataa tccgggagca ttttcaaaat aaaaccattt tgcggcagcg gtatctttta
     4981 ctgttttgat aaatatcccg acatgagaag ccgaacccct gtagaagtgt tgaacttgtt
     5041 tttcggatga ggtgattata ccgctgttac tgcaggaaac agcgtatgca gcaaaaagaa
     5101 aaataaaacc gccttttatt actgtgttca taataaatgg gatagcaatg aataatgaat
     5161 ttaccgttta tttcgataat gggtcagtca gtcattttct ggttaattct atggtatgca
     5221 atatcaactc cagtccgccg gatagcccgt gaccgggaat gactattttg gcgtcgggga
     5281 attttctgat cagtttttca agcgtttcag ggtaggcgtc caggtccccg tcggcagtgt
     5341 tccccaggtt ctttgcgtca aggctcttta ccatgcaacc ggcaaacaat atcttttccg
     5401 cggggatcca tacgacgatg ttatccaggg aatgcgccgc tcccgggtag taacattcga
     5461 tttgctgatc gccacacttc aggagcaacg aatcggtaaa cccgtgcccg gggaccggca
     5521 gattattcaa tttggcaagg ccaatcgtca tccggttcgc ataggactca acattccggg
     5581 attgcaggaa tcccaatcct cccaggcagt ctttatgcca gtggttcggc acaaacccgg
     5641 caatctccag tcccatcggg cctgtgatcc aatcgtacaa ttccttcgtc agggaatcat
     5701 cccatggagt gtcgaaaaga aaagcctcat ggccgctgat aaaaatcagt ccgttggcag
     5761 atacccggcc catccccggt acctccgcat aagaaacatg tatatacgca ttttccgaaa
     5821 tccggatcag ttcaaggtct tctgaaatac tgacggactg atatctgggt tgcgagaacc
     5881 cggtgacctg aatccagccg gccagaataa atatcatcaa cttcttcatg aaactgtttt
     5941 catgttgttc aacagacagg cccgtgtatg tgtttttctt tcaacagttt atattgcctg
     6001 gattttttgc ttgcgtgtat ccccggtgtg cgacagggaa agcccgcctt gttattaatg
     6061 tccgaccata ctgccctgat cctgtatttc ctttatctat gcaagttatg caaaattctt
     6121 tttaatacgc ttttggattc gtacaaagga taagtaaaat gcaagcgcca ttagcgggat
     6181 cattgcgcaa gcgagacatg cgctgaaaat tgtgtagttt gccgcaggcc tacatcgcag
     6241 gcgtcaggag acgcgagcga gcaaaggctc gcgcggtagc gaaggaactt caatcataac
     6301 aattcaaact tagtaatatt ctcaatgatg aggttggtta ccttattctt gtaaagaagt
     6361 ttctctactt tttctgtaac aaacgtaaac atagattcct ccggtgtgaa gacatctgaa
     6421 ccatcccagc tacttaagtc aaaataaagc cctcttttag cctctactgg ccttcctgtt
     6481 gttgtgaagg gagctttaat agatgcttca ctcttttcat aatctatagc agaacatcgc
     6541 cctgtaaccg tgaacaaata atactcatac ccctcataac cttttattct cacaggaata
     6601 ctgctccacc cgcttatagc attattgtta aatagttcaa ttatcttttt tgagaccatg
     6661 taaagaccgg catacccgga attcaaaaaa tcaaaagtct tggaaccaag acttggtata
     6721 cattctacaa catgttcagg aataaattca cctttaaaaa gcttataaaa atcagcattt
     6781 aacccaggaa ctaaagtcat tgcccctcga gtatcttttt ctgaaataac ataaacatct
     6841 gtatgtgtaa tttgtacttg ttttactaac caaatatgat tttaagtctt aaagcctctt
     6901 taaaaatttc tgactgggtt gctccgggat ttgtgataaa aaaatcacgc caagcattat
     6961 tataatgatc gccaccacac caagcacact caagatggca cgatcaccag caacgcgtta
     7021 atttattaca tatcttctct catcatcatc tctttaagct cttttatcaa ctcaggaata
     7081 aaagactggt ctatgttata cttaaattct aatttgcacg tagataaatc attatatact
     7141 tcacctttga ttttgatctg gctaccctct tcagataatt ttagtgtaag tttacctgaa
     7201 aaggggttaa atgagatata attctgctct ccattataca ttttttctaa tgataattgg
     7261 agatttgtaa aatcatattc ttcagcttca aaattagttt ctatatggaa aaaatcgaaa
     7321 tcaactttgc ctttaaaagt gactattttc agagcctcag ttttaacaaa ttggaaatct
     7381 tttaattgta ttatcagatt cctttcttta tcatagaaat ttatagtatt catagttcta
     7441 gaatttaatt aagaatgttg gccattcata aaaacatctg tgaaaaatta taccaccttc
     7501 gctcacgcgc gcaggttgcg cgtgcgtagt tatacttact acaaacttaa caatattgcc
     7561 tcaatcaaga atcgtttccc ggcataattt gtcatcggac tgaatatata tgcaccagga
     7621 tttttgtata tagtccggtt tttcagttat gaaacggtta ccgaacagcc atttcgcctt
     7681 gctgccgtaa taccgcaggc tttcacctca tccgcctccg gcgcctcttc ccgatgaatc
     7741 gggacaagcg ctcagggaga aggcctgccg gccgggcaga ccctgaaggt tttcaaaacc
     7801 ttcagggtct attgaggcgc cgatgcaatt tacccgatgc tgttgaaaat gaatgaacgg
     7861 caaagaaacg cccgatcctg ttcataaaaa aagatctgac agatttctac aacctgtcag
     7921 accttttata agctaaccac acattcgggc taattcttta taattttgac actttcacgt
     7981 ccgtgcggtt ttatgacttt catcagatac aacccttgcg gctgatccga taaatcgaaa
     8041 gtggttgtct cgccattcca ggatgactgc atgacaagct tacctgtgtt gttgtaaata
     8101 aacagcgtgg aattccggta ggctttttcg gaagtggccg atagttcgaa tttcccgtcc
     8161 gtcggattag gcacgacctt tatggcgccg gggaagtaat ttatcggttc gtctttcccg
     8221 acaataacga tcgggctgtc ggaataagaa accaggttgc aggccacatc cagggtggga
     8281 ttaaaaccat cggagcaatc gctgcacgta accccggtaa cacaatccgg attgttcggg
     8341 tggcagttat ccatatactg ctcgtaaaac ttatacatca gcgaaatatc gccccctgaa
     8401 acataaggcg tcatatcata atcgaaccat ttggcgatgg agcccgggca ccagccggcg
     8461 cgatcgtatt tatatgttcc ttgctgattt tcgcaactat caggattggg agtgcaatcg
     8521 caccagttta attggctgaa agtattatcg ccattcacat agatatggtg agttgcctta
     8581 tagaattctg cagcattacc cgtgttaagg tttccccagc cgtgtccggt cgaaatcagt
     8641 tttagcttgg atgccactgc gtttgccggg tatgagtaat aaaaggtatc taccggctgc
     8701 aggttggcat agtccccaaa ggggaagatt tgattccaca cctcagtaat atagctatac
     8761 ttatgcgttg gtactcctgc ccggtataaa aagctgagat cataaaggta accattgtca
     8821 agggtagtgc aatttatccg gaacctgatt ttgccctgga gtaccgacat gtaatcagta
     8881 aggtcaatac tatgggtgca agctttgcca aacggagtaa tataacggat gatttctatc
     8941 catctgccgt cgtgtccctg ggcttcaacc catgcaaccc tgtcatattc gccacatcct
     9001 ccggaagggc aattaacagt taatacggct gaaattttgt caaatgctcc gagaaatgaa
     9061 ggcaactcag cttcaacttc ctgtgacact ttaactgaac taagccaaat gtcatctgcg
     9121 gcctgcactt ccatatcttc cacatcctgc tgaatattaa ttgaataagt tttggttgca
     9181 gtcgccccgt aattgtttgt ggctaaaacc tgcagcgaat aagtgccata agcaggcggc
     9241 gtccaccagg cagtataatg cgtgttgtaa aaatcctgag cctgtatcgt ttgcccgtca
     9301 atcctgaact gaacgcctgt cacatggaag agttcgggat aagtgatttt gctgatggcg
     9361 gccagttgta tttgcgagag cgacgggaca tatacattgc ccgcgagcgg attgcgtaat
     9421 tcgatttccg ggatatgcag cgcaggatcc agtaccatga atgaatttat tacgggatca
     9481 gcagggttat attgtgaatt gcccggttgt gatgcttcga tgaaaaccat tcctgtgtca
     9541 ccggtaaggg taacaatatt gccgttcagg gtggccgggc cggacaagat attgaactgg
     9601 acttcgagcc ctgaagttga ggttgccgaa atggcaaacg gcgctgtact attcagttta
     9661 tcgggaatct gggggaaaga aattgcctga taggatgaat caagggtgcc gttaaagttt
     9721 gaggtgggac ctgttaaagc aaagttcaag aggtcgccgt tccgttcggg ggcatatacc
     9781 tgtgtgatga gttgggtgat ggatgtatta tcgcctcccg gcactccctg gttaaattta
     9841 taatacatct gcagatcagc ttcggtaccg gtcagttcgt ttgccatcat ctcctggatt
     9901 tcagcctgcg tgagaccgcg tttccagacg gagacctcat cagctctccc gccgaaaata
     9961 aagttataac cgccaagcag gcttttaccg atagcgaaag gtactgttcc gtcggtgagt
    10021 acgccggcgg cagctttact gcctttgaga ttgccgttga tataaagttt aactgccgac
    10081 ccgtcaaata cccaggcaat gtgctgccac acctgcggaa tgattgtatt tgcaggggct
    10141 gcatactcgt ataacgttcc tgtggaactt ttatacctgc attcaagttt gccgtcgctg
    10201 agctgaatta catagaattc ggtcgcgcct cccctgaaac ccatcatgcc ctgtccgtaa
    10261 actaaggcgt ttgtataaaa ccagccggcc atggtgatcc cctgcgttga attggtaatg
    10321 tatgattccc cgttgggagt aaccacataa tcgtcgatac catcgaaatc gaggtactga
    10381 tttgtctgcg cttcagccat cctggctgtt aatatcagca ggatgaaaag caacgtcctg
    10441 aatattgtat ttctgttttt catgggagag ggattaatgt gaatatattc tatttgttat
    10501 gtttattttg cccaccatat tttcgtctga ggcgagtcgc ctccctgcct tgagatggcg
    10561 tcgttgcaat tgacagtgtt gtattcatat tccgatggcg ggtaaggcat acggaaatgg
    10621 tttatgggat cgccttcgcg gggtgtcatg ccggtcctcc tggtaagggc ataagcatcc
    10681 cagggttgcc tgaacatgtc tatccagcat tgagtataaa ttaaacccag gaactcttcg
    10741 tcgttgcttg cattccatga accgaaatgc gttaacacac ttcccgaatt tacattctga
    10801 ggaattgtta tcatatcctc aaatttgact ccggatattg gcagttgcga tgaagcggct
    10861 acattaagcc accattttac ggatgaaaga atgccgttaa ggtattcaat atcagccata
    10921 tctctgtcct gggttacgcc aatacctcgt aagtatgcct cagccttgat gaaatgaacc
    10981 tcggctccgg tcattaaaat aatcggcata taatcctgat cgcgaatgag gaaataattg
    11041 aaaggcgaat agttacagtt ttgtttcagg gtgaaatttg ctgcatcgtc gcggtatgat
    11101 tcgtatggaa ggccgcctga ggaaggggtc gtattgtcag gtatctgtgg atagggcgtc
    11161 cattgattgt taacatccgt ttcaaagaaa atataagccc tgggatcaaa tattccgctc
    11221 ccgctggggc tgtcagttga ggaaaactgg tgccagatat tacttcccat ccggagtccg
    11281 ttatgttccc ggaacgacca gttaaggctt tcgtttttat aacctatctc gagcggccat
    11341 aggcatgcgc tttcgagtac cgggttaaca aaatcataac ccagcaatac agggcggccg
    11401 tcttcgatga ttttcttaat aatattaccg gcaatttcag gctcctggtt caccattctc
    11461 atcgcataac gcaggcgcag ggaattggcg aatttctgcc acattgtgag gttgccttta
    11521 aaaagtttgt caaaggtttt aaaactcgta aaaggttcaa cttcagcagt tgtatcgcag
    11581 atcagtgaat cggcaaccct gagtttttca agcatactga ggtaaatgtc gcgctgggta
    11641 tcgtattcgg gatgaagata ttcgaggctt tggaagccgt aaccggcttt gcttacaggc
    11701 atgtcgccga acaggtcggt catcttgaat gtcttgtacg cgaaaacaat gtcgagcatt
    11761 gctttcatat tattgaccga tttggaaggt tccgcggtat taaaccgttt ctcgagttcc
    11821 ctgaaggatg gcagtatttc ataatagttg gtccagaggg cttccgtccc gatggtataa
    11881 tttccccacg catctttggt gagtgcagca agttgggtct gtttgtaaag gatttcgtta
    11941 ttgatataaa attgttcatt tccggaaagt accagcgatt cgataattcc gttgaacagg
    12001 gcgccatcgc tggcagtagt gaatcctaag gggttggtat taatctcgct aaaatctttg
    12061 gtacatgatc ctatgaataa aagtgcaggt atgatcagga gggccggaat gtattttttc
    12121 atggtagttc tttgttgaag tttagttgac cagtcgtaca gttaaatgat taaaacgagg
    12181 catttacccc aaaagagaag gattgggtgc tgggatagga agcccattca aatccctgtg
    12241 cattgcctga acccattatg ccttccgggt ttattttatc gggaagcgtt gtataaatgt
    12301 aaaagaggtc gcgcccggtg aggttaatgg taagagcctg gaatactttt attttttttg
    12361 tcaggctttc cggaattcta taggacaacg agatttcacg cattttgacc caggtgtttt
    12421 ccagtattcc cggtttagag atgaattttc cccagcctcc ggcattgggg agatacttgt
    12481 agtaataatg cactactttg gtattttttg tcccgtcggc ataaacgcca tccaaaatca
    12541 cgcccacatt ccttacattt ccttccggat cggtatacgg aaggccgcct ccgtcacgtt
    12601 caagcaaggt ggatgggctt tgcccggttt gcagcgaaat cacatacgat ccgcaataga
    12661 tgtcgcctcc ccattttgtg tcgaccaggg cggataacct caggcctttc catgagatat
    12721 ccgcatttac ggcgcccgtg aaatcaggag acgcattccc gacaggaacc cggttatcgg
    12781 taatcaggaa tttggtgcct tcgtcattta aaatgcgttc ccctttatca ttataaagat
    12841 aatcgtagcc gacaatggtt ccgtagtcgt cgccttcttt cagcgctatg gccggtccgt
    12901 taagtcccca gatttcagca agcagatagg tatcggaata atctcccaga ctgacgatct
    12961 tattcctgtt acgtgcgaaa ttgaagcctg cttttattat cagattgttc gtctgaaccg
    13021 gtaccgtatt gatgattagc tcaacgccgc ggtttgaaag ggtgccttcg ttaatgctta
    13081 tccccggagc tcccgaacta acgggtaccg gaaggtcgag tatctggtca aaggagtact
    13141 tgtagtacca ggtaaagtca atatcaatcc ggttttcaaa aaggccgata ttgacccctg
    13201 cttcatagga attgacccgc tggggtttca gccggtaagg gggtatccgg gaaggaaagt
    13261 atgaggtctg gtctcctccg aagaagctgg tagtataata gaaatgagcc tgatacggat
    13321 tgccccctga tgcagtttgc gaaatgccgc ccctgatttt aaggaaatta agccacggta
    13381 aatgtttttg cagttgtaaa gcctcggatg caatataact ggcagatacg gaggggtaga
    13441 tgaaggaatt gctgtaagaa ggaagtgtgg aagaccagtc gttccgggct gtaaattcga
    13501 ggaacaggta gtctttataa gcgaagttga ggaacgaata taccgaatta ttcctgattc
    13561 tggatatccc ttcctgcgtt gccacttttg aaagggcatc gccggtctcc tcaaccatta
    13621 tttgcccgtt ctcatctttg acataattgg tttccgtata gttaaagaag gtatacatgt
    13681 tgggatagta ccacgttccg gagtgcccgt aaatcgagta ataattataa tcccagcggt
    13741 ttgcgcctgc cgtaaattta acggtgaccg gcgaattaaa aatcattttc ttttcagccg
    13801 ttagtatcag gtcgaaattg tcgttgtaac attttgagag ttcgtttgaa taagagccgc
    13861 ccaataagcc tataacatca gtaggcttac tggttgccgt cttactttca acgacataat
    13921 cacgtccggc ccttccggtg aagttcagcc agggagtaac agcgtatgaa agcgcaaggg
    13981 agccagtata tttgtccctg ctgagttggg tattgttgtt ataatagtcc caccaaaggt
    14041 atctgtcgac atacaggaat ggatatccgt caagtttgtt ctgtgacccg tcagccaact
    14101 ggtaattttt caggtcaagg ttctggtaac tgcggggcca ggaatacaga tatcctttgt
    14161 tgaacgaatt ctggtcttcg cccaggatag gactgtttaa ccggttgaac cttacatagg
    14221 taagcacggc gtcagctttc actttttcgg agatattgat cgtggtgcct acattaatgg
    14281 tggtgcggtc gtaattgctg ttcctgataa tcggtttgtg atcctgccgc gtaagcgata
    14341 ttcgcagcgt ccctttttcg ctgccgccgg tagcttcaat attgtgggtc cgggtgaatc
    14401 cgtcctgata gggttctttg aagttgtcgg gctggggcga gtagttccgc atttctccat
    14461 cccaccattt aaccagttga cctttcatct cgggccccca cgaaacggat gatccatagt
    14521 atccgaattc ttcggaagtg cttgatgttt ttccttcctg gttaatgacg agcttgtcat
    14581 ttccatagac gccgggatat tgaagaatgc cgttttcatc aacagggaat gcaggttcgg
    14641 tgaacgaaac aggaccgccg gcgccgtatt tgttttgcac ttcgcgatat ctgtatggat
    14701 gggtgatttt ttcttcaaag ttgtatgtaa ttcccaatcc gctctgtttc tttccccttt
    14761 tggtggtgat gaggatcacc ccgttggctc ccctggatcc gtataaagcg gatgcggcgc
    14821 ctcctttcag taccgagtat tcttcgatat ccagcgagtt caggtccgag atgccgttgc
    14881 cccagtcaac gccgcggcca atgtctgtga ggccttcagt gttttccagc gggacattgt
    14941 cgactacaat caggggctcg ttgttgccgc ccaggttgtt gttgcccctg atggtgatgc
    15001 gcgttgtgcc gccgtcgacg ccgtcgccct gcgaaaccat gacgcctgcg ctacgtccgg
    15061 caagggcatt tatgatgttg ggggctcctg cgtttgcaat ttgtttagtt tcaatttttt
    15121 cggtggaata accaatttcc cgcttctggc gttttacacc gagggctgta acaacaactt
    15181 cttcaaggcg tgtagcggcc ggtttcatca ctacattaat tacactctgg ttcctgacag
    15241 tgatttcctg tttttcatat cccagaaagc tgaaaaccaa tatattggaa gatgaaagac
    15301 gggatagtgc gtatttaccg ttaatatcgg tagtagtgcc ggttgaggtg ccttttatca
    15361 gaatggatac tccgggtaag gttgatccgt cttcggatga ggtgaccgta ccggtcacgg
    15421 ccatctgttg agcgcttgtt agcaaggaga aggtgagaaa tagaagcagg agttgagtag
    15481 attttcccat attcaggttt ttaaatttta aaatctaatt attatcgcaa gcctttaaac
    15541 atttatcccg caagcctttc ataattaaac ttttttggtg ctttcaatta atgcggaagt
    15601 atttaattta aaggcttatg gagcccgggt ggcaggtgat atcctgtaaa aatataaaga
    15661 ttttgagaag cgcaatgctt ttcactggca tcagaaaaga attttttctc ccgggcacgg
    15721 ttgaagtctt aataagcgtg acagttgctg catcagcagg gggtaaaaag ggtataattg
    15781 ttcttcttct gtattcaaaa gtaatcattg ccggttgatc cggaatggta tggatgaaag
    15841 tacgatgctg acgccgacga aggctgcggc aatgatgctt ccgggccctg cttgatttcc
    15901 cggcattcag gcaaaccgga cgtttgagag gattacagat gccaatcaac accaccggtt
    15961 gatcatggag aaatctctgt ttattccttc agcctgcggg tcatcatttg ttcaacaatc
    16021 gagaagagtt tcacagggtt atgtacccgc cagggaatgt tattaaattt tccgcccacg
    16081 gccagatagt aatcaatatt cgactgttcg atttcacgga tcacatgttc gcggaagttg
    16141 agtaaaacaa tccagccgtc gtcgatttct tcgaaccatt cctggtaata ggaatcgtcg
    16201 gaagcatgaa attcgagtac gttttcgcca ataaatatga attttgtaat tccttgttcg
    16261 ataagatatt caattacatt gcgtttcagg tacatgatgt cgttgtacag gcaatcgttc
    16321 cattcgccga aaagttcaat gatacagaag ttcttaacat aatcggcata taaaatttta
    16381 agataaagcg tagccgaacc aaactcatcc cactgcggat gtatcaggta attgtatatc
    16441 tggtgagtga attcaaattc agaatactct ctcccgtaaa agggcgatcg ttcatcttcg
    16501 gaggaaatat agaggagcct ccatgaataa aacggttcaa tgtcgtgcat ccggcagatc
    16561 tgggttgagg cggttttgct gaggtatggg catcagtgaa atgcagccat cagcaggttg
    16621 atatcctgga aaggcaggcg gtaggtatcg ctgatgaatt tattggtaga aatgccgttg
    16681 aaaatatata cgctttgacg gacgccgtag ttggaatgca gcacctgttc gaggccgcct
    16741 tcttccccta ttttttccag gataggcgtc aggatgttgc tcagggcgta cgatgccgtg
    16801 tgcggtactt ttgaggggat attcggaacg cagtaatggg taacgccata ttttctgaag
    16861 acaggattct gatggtcggt aggatgggag gtttcaaaac agcctccctg gtcgatgctt
    16921 acgtcgatca gtaccgcccc ttctttcatt tgttgtacca tttcttcggt aaccaggcat
    16981 ggagtgcgtc ccacggtgga gtgtaccgcg ccgatcacaa catcggctgt cttcaggttg
    17041 ttaagcagga tgcgcggctg cagaatggag gtgaatatcc tcacgttcag gttgttttgc
    17101 agccttcgca gcttgtaaat cgaattgtcg aagattttca cgaaagcgcc cattcccagg
    17161 gcggcgcggg cggcaaattc gccaaccgtc ccggccccga gtataactac ctcagatgga
    17221 gtgatgcccg agaaaccgcc cagcattttt ccgcgtccgt attcggcatg gctcatgaac
    17281 tcggcggcaa gcagtattga ggtgttgccg gctatttcgc tcatggcgcg gatgatagga
    17341 aaagtgccgg ttttatcctg aaggaattca aaagcaaggg cagtcgattt ttttgccgtc
    17401 agtttcctga aatattcttc agtcagtccc gggtgataca gcgaggaaat aatggcctgc
    17461 cggctggtca gcatgtcggt ttcgctgcag tcgggcggtg cgatttttac cacaatgtcg
    17521 gcagtatata cctgacgcac atccgggact atttcgcctc ccatttcgct gtaatcatgg
    17581 tttgaaaaat gggcggattc gccggcttta ttttcgatga taaccctgtg cccttgctga
    17641 accagcaaac caaccgcatc cgggaccagc gcgatacgat tttcgttgag cgaggtttcg
    17701 cggggaatgc caattgtaag ctgccggggc gtttttttta tttccagcgt ttcttcacgg
    17761 ggcatgtaag ccgcaccatg cagaaggttc tggaggttgt tgtttgataa gtccatgacc
    17821 tgttttcttt cagaaagtaa aatatcttgc ttcagtttcc gcgtcagttt ctatcctgac
    17881 ttcaaccgcg tcagagggca gcaggttttc gattttttca ggccattcaa tcaggcagta
    17941 gttgccgctg aaaaagtaat cctcataacc gatgtcgagc atttcttccc agttttttaa
    18001 cctgtaaagg tcgaaatgat aaatgctctg cccgtttttg gcaagatatt catttacaat
    18061 agcaaatgta ggactgttta cgatggtttc aacaccaagt tgcctgcaaa attctttaat
    18121 aaaagtggtt tttccggcgc ccatattgcc ataaaaggca aaaatccttc ttccgggcca
    18181 ggcgctgaga agcgactttg cgatgtcggc aagctcgcca gggcttttac aggtataagt
    18241 ttgcggcata gtcaggttat ttggctttca gaaaggcgat cggaacaatc atttcttcca
    18301 tggaaatgcc gccatgctga aaggtattcc ggtaatagtt gacgtaataa ttgtaattgt
    18361 tggggtatgc gaagaaatcg tcgcggcggc tgaaaacata ggcagaactt acgttggacc
    18421 gcggcaggaa gatgtcggcc ggattcttta tctcgaatac ttcgcgcttg tcgtaattga
    18481 ggctgcgtcc ggttttgtaa cgcaggttgg tgttggtatt gcggtcgccg ataattttca
    18541 cgggattgct gacccttaca gagccatgat cggtagtaat gatgaggttg acttttttct
    18601 cggccaggaa acggataatg tcaaacaaag gggagtgtgc aaaccaggaa accgtgaggg
    18661 aacggtaagc ggcttcgtca tcggcaagct cgcgtattac ttccatttcg gtacgggcgt
    18721 gcgaaagcat gtcgacgaaa ttgtaaacaa ttacgttgaa tttgtttccc atcaggttag
    18781 gcagtgtttc cgccagtttc tgcccgctgg caaggttcag gactttggta taggaatagg
    18841 cgacattttt agcctgacgt ttcaggtttt cgcccagcaa ttcgttttca aattgattct
    18901 ttgtgccttc ctcgtcttca tccacccagt atttcggata ttttttttct atttcactcg
    18961 gcagaagccc tgcaaagagg ctgtttcgcg catattgcgt tgtggtggga actatggcgt
    19021 aataaacctc atcgcgctca accctgtaat attgttcaat gatgggctgc aacactttcc
    19081 actgatcgta ccgcatattg tcgatcagga tcataaaaag gttctggtcg gtattgatca
    19141 gggggaaaac tttatcgcgg agcagcgtat gggattgaac cggcttgtca acggccttgc
    19201 cgtttaccca gtcaacataa ttgttttcaa caaatttgca gaagacctgg ttggcttctt
    19261 ccttctgcat gcgcattaca tcgacgatgc cctggtcctg cgatttttcg agttccagct
    19321 cccagtatac cagcttttta tatacctcgc cccattcttc gtggctcagg cgcccggaaa
    19381 attccatgcc gatattccgg aattgctgct gataggcata agtcgttttt tcgctgatga
    19441 gccgcttgtt ttcaaggttc tttttaacgc tcagcaatat ttgtttcgga ttcaccggtt
    19501 tgatgagata atccgaaata ttcgacccga tggcgtcttc catgatatgc tcttcctcac
    19561 tctttgtgat catgacgaca ggaaggttag gatggctggc tttgatgatc gacagcgttt
    19621 caatgcctga aagccccggc atctgttcat caagaaatac aatatcgacc ggctggcttt
    19681 tcaacaggtc aatggcttcg tcaccgttgt ttgtggtgta aacctggtat cccttttccc
    19741 ttaaaaacag gatatgcggt ttcagcaggt ctatttcatc atcagcccat aggattactc
    19801 cgttttccat gatttgcttt ttattgtacg atgactatga attcgtttta acagttattc
    19861 tttatctttg ttttatgcaa ttgaccagcc ttcagacggg ttaaccggta tcgtttcaat
    19921 aataatgccg gttctcctgt ttttattgca gggccgcact tttaattcat agtctcatat
    19981 acaaatttaa caatttttgt gagccatttc attcccaata aacgaaaaat catcaatgat
    20041 ccgttgcacg gtttcatcac cgtgcccgat gaacttacct ttgacgtgat tgagcaccgg
    20101 tactttcagc ggctgaggcg gataaaacag ctcggtttca catcaatggt ttatcccggc
    20161 gctttacata cccgttttca tcatgccctg ggcgccatgc atctcatgag gcaggctatt
    20221 gaaacgctca ggatcaaagg atttcggata accgatgacg aagctgccgg cgcatcgctc
    20281 gctatcctgc tgcatgatgt agggcacgga ccgtattccc atgcactcga aaactgcctt
    20341 gtttacggaa tgaaccacga agatatcaca gtgttcttcc tggacaggct gaatgaggag
    20401 tttgaaggga aactggaaac cgccatcagg atttttaagg acgagtaccc ggaaaaattt
    20461 ctgcaccagt tgatctcagg ccaactggat gttgaccggc ttgattacct tatgcgggac
    20521 agctttttca cgggcgtgag cgaaggcgtg atcagcacag accgtatcat caaaatgctg
    20581 accctggttg acggtgaaat tgctgttgaa ggtaaaggca tttattcgat tgagaaattt
    20641 gtggttgccc ggcgactgat gtattggcag gtgtattttc ataaaacggt gcttgccgct
    20701 gaatataccc tgttgaatat actgagaagg gcaaaatacc tcagcgagcg gggggcgcag
    20761 ttgtttgcca cgccggcatt ttctttcttc ctatccaatc acataacagc cgatgatttt
    20821 cgggacgatc gtcagatttt ggaactcttc tcgcaacttg atgattttga tattttttcg
    20881 gctatcaaag tctggacatc ccacgaggac aggatattgt ccatgctgag ccgggccctg
    20941 gtatacagaa acctgccaag ggtggaactt caatcgggcc cgtttgatga ttcttttgtc
    21001 ggggagctga aaaaacgtca gaaggcccag atgcaactct ccgacgagga ccttgattac
    21061 tttattttca ccggaagcat tgccaatgat gcctatatcc cattccgtga taagatcaac
    21121 attgtagaca aacagggcca tatttccgat attgccatgg cttcggacca gctcaatgtg
    21181 gctatacttt caaaaacagt cacaaagtat tttatctgtt atcccaaaag tatcggaata
    21241 aaggaaggcc ggagcaatgg atgattgtcg gttgcctggc ggcgtattcg ttaatattcc
    21301 tatatttgca ggtcacaaga aaagtgttta ttatcaacca taacatactt cctttatgaa
    21361 attcaaggca agcgagatag ccgccctgat aggcggtgcc gttgaggggg atcctgaaat
    21421 agttatcacc aaaatagcga aaatagaaga cggcgaatct caagcaatta gtttccttgc
    21481 taatccaaaa tatgaaccgt tcatatacaa cacccagtca tcggttgtta tcgtaaagac
    21541 cgacttcagg ccggaacacc ccttatcatg cacccttatc aaagttgacg atccctacac
    21601 cgctattgcc gtgttgcttg atatgtacaa caagatgaac aaaccaagga ccggcatcag
    21661 cagccttgct tttatttcag gatctgccaa aacggggaat gacgttttta ttggcgagtt
    21721 cgcctttgtt ggtgaaaatg ctgtgattga aaatgaggtg aaaatttatc cgcaggcata
    21781 tatcggggac aacgtaaaaa tcggcgaagg caccatcata tatccggggg ttgtcatcta
    21841 tcacgattgc gaaataggaa agcactgtac tgttcatgcc ggagttgttc tgggcgccga
    21901 tggttttggt tttgcgcccc aggatgacaa caactacatg aagattgccc agatcgggaa
    21961 tgtggtgatc gaagacaatg ttgaaatcgg ggctaatacc accatcgatc gcgctacgat
    22021 gggatccacc attatccgta aaggcgtgaa actcgacaac ctcatccaga tcggccataa
    22081 tgtggagatt ggtgagaaca cggtggttgc agcgcaagcc ggatttgccg gttctacgca
    22141 tctgggccgg aattgcatga taggggggca ggccgggttc gccggccaca ttgtaattgc
    22201 cgatgatgta aaaatagggg cacaggcggg cgtacccaac aatattccga aacagggcag
    22261 tatcatgctc ggcgcccctg ctattgatat caggcaattc agacggtcgg tggcggtttt
    22321 caatcatctc gataaacttg cgcaaagggt atcggaactc gaaaagaagc tggctgaagc
    22381 aactaaccaa cctgaaggga aatagagtca tacatcattc tatttaacta acgaaaacac
    22441 tgcagaatgg tcgaaaaaca atgtactata aaacagccgg taagcctttc gggcgccgga
    22501 ttgcattcag ggaaagatgt taccttaacg ttcaggcctg ccgaagccgg gcatggaata
    22561 aaattccgcc ggattgatat ggatggacag cccgttattg atgccattgc cgattatgtt
    22621 gttgatactt cacggggaac cacaatacag caccaaggcg tcagggttgc cacgattgaa
    22681 cacacgctgg ccgccgttgt gggaatgggt attgataata tcctgattga tgttgacagt
    22741 cccgagatgc cgattatgga cggaagcgct gcagcctttg ccgaagccct ttcaaaagcc
    22801 ggaatcgttg aacaggatgc cgaccgggaa tattttgaag cccatacaac gcttacctat
    22861 tccgacccgc accggaagag tgaaatgatc ctggttcccg aggataactt ccgcgtttcg
    22921 accatgatcg atttcgaaac ccgcattttg ggcacacaat acgcatccat cgatgatatc
    22981 agtaatttca gggatgaaat tgccaattgc cgcacctttg ttttcctcca tgaactagaa
    23041 tacctcatat caaacaacct gataaaaggc ggcgatatca acaacgccat tgtgtttatt
    23101 gacaggctgc tcggcgatgc cgaacttgac aggctggcca aatattttaa caaggataaa
    23161 attgaagtcc tgaaagaggg tatactcaac aacctggaac tgcgtcacca taatgaaccg
    23221 gcccgtcaca aactgcttga tgttatcggc gacctggccc tgatcggtat gcggatcaaa
    23281 ggccacgtga ttgcaacccg tcccgggcat gccgtaaata caagtttcgc caaactggtg
    23341 cgcgatcagg tcatcctatc caagcaaaaa ccaacattcg atatctttaa aacacccgtt
    23401 tatgatatca acgatatccg gcggttcctg ccccacaggc cgcccttcct gctgattgat
    23461 aaaatcattg aaatcagtga taatcatgtg gttggtgtga aaaatgtgac gatgaatgaa
    23521 cctcatttta caggacattt tccggaagaa cctgtgatgc ccggagtatt gcaacttgaa
    23581 gccatggcgc aaaccggcgg aatcctggca ttgcataacg tgcctgatcc tgaaaattat
    23641 gcaacctatt ttctgaagat cgacaatgtg aaattccggc ataaagtggt tcccggagat
    23701 accctgcttc tctctcttga attgatatcg cctgtcaggc gcggaatatt ccacaccaaa
    23761 gccaacgcat ttgttaataa tgtgctggtc acggaagctg aacttgtggc ccagatcacc
    23821 aggaaaaaaa ctgtcaacac cgacaacaat actgtaaaat catgaaccaa cccttagctt
    23881 atgtgcatcc tcaggcaaaa attgcaaaga atgtagttat tgagcctttt gttaatatcg
    23941 agaagaatgt tgagattggt gaaggaacat ggatagggtc aaacgtaacc atcatggagg
    24001 gcgccacgat cgggaaaaac tgccgtattt ttcccggcgc cgtcattgcc gcaattcccc
    24061 aggatttgaa atataacggt gaagaaaccg tagtcaggat aggcgacaac acaaccatcc
    24121 gcgaatttgt taccatcaac cggggaacca aagccagttg ggaaaccgtg atcgggaata
    24181 actgcctcat tatggcgtat gtgcatatcg cccacgattg ttcggttggc aataattgcg
    24241 tgcttgcaaa ttcggtggcc ctggcagggc atatttcggt ggaagactgg gccgttatcg
    24301 gcgggcattc ggccgttcac cagtttgttt ctatcggaca gcattcaatg acttcgggtg
    24361 gagcgcttgt gcgtaaagat gtgccgccat acacaaaagc agcccgggaa ccgatttcgt
    24421 ttgtaggtat caacagtatc gggttacgca gacgcggctt tacttcagaa aaaatcgctg
    24481 aaattcagga aatttaccgc atcctttacc tgaaaggcta taacgtgacc caggcagttg
    24541 gaattattga aacctcaatg cccccgacct ccgaacgcga tgagatcatt tccttcatcg
    24601 cccgttcgca acgcgggata atgagagggt tttcttcagg aagagaatag ccggtaaaat
    24661 ctataagcct gtaatctaag gcaatccggg tcaaacagtg tcacaggcag atactcatgg
    24721 aaatatttct ggacaacatc agccgtaaat ataaccacga gtgggttttc cgaaatttat
    24781 ccttcacatt ctcatctgga aattcgtgtg ccgtgcttgg tcctaacggc tccggcaaat
    24841 ccaccctgct gcagatcatt tccggtcacc tgcatcctac cggcggcacg gtgcaatacc
    24901 ggctcaacgg atcaatgctg ccggatgagc aaatattcag gtatatcgcc attactgctc
    24961 cctatattga attaatcgag gaatttaccc tgcctgagat gatatccttt cacctgaagt
    25021 tccgaaactt aaggcatggc atggatgtaa aagagttcat ggatatcctt gatatcggaa
    25081 gcaaccgtga taaatgcctg aaatatttct cctcgggtat gaaacaacgg gttaaacttg
    25141 cgctggcaat actgagcgat gtgccggttt tgctgctcga tgaacctgcg acaaacctgg
    25201 acgcttcagg tgtcgcatgg tacaggggcc ttgtggcgga acattgcgcg gaccggatcg
    25261 cagttatctg ttccaactcg caaccggcag aatgcgattt ctgccatact tcgttgctgg
    25321 ttgaaaacta taaggggacg cggtaaaaca gggcgtcatc aggctgttat agtttgggat
    25381 gaccggcatc tgccggataa tattatcagt ttatgattat gagtaggtta cagattggca
    25441 ggaattcctt aattttgcac actttttaac aacaaaaatc aattctatgg ccacaacagc
    25501 tgatttccgt aacggattat gcatcgaatt caaaggtgat ttatataaaa ttgtagaatt
    25561 tcaacatgta aaaccgggca aaggcgctgc ttatgtgcgt accaaactga aaaacctgaa
    25621 atccgggaaa gtgctggaca atacctttga tgccggagaa cgtatcgatg tggcacgtat
    25681 cgaacgccgt ccttaccagt tcctgtacaa agacgattca ggctatcatt tcatgaatac
    25741 gcagacgttt gaacaaacat tcgttgagga agaaatggtg gatggccatc aattcctgaa
    25801 agaaggccag gaagtggaaa ttatgtttca tgctgacacc gaatcgatac tttcgtgcga
    25861 attgccatcg atcgttgaac tggagatcac atacaccgaa ccgggacttc gcggagacac
    25921 ggccaccaat accctgaaac cggcaactgt tgaaacaggg gccaccgttc gggttccgct
    25981 gtttatcgaa gccggcaccc gtatccgtat cgatagccgc accggcgact atatcgaacg
    26041 cgccaaataa gcaaatactc agatttatac tgcacaaccc aacagggccc taccccatga
    26101 agtttgccct tcctcttact ttacagcaaa ttgcagcttt tctccaggct gaattttccg
    26161 gcgatcccgg tttccctgtg aaaggcctca acgaaataca catggtggaa gcaggcgacc
    26221 ttaccttcgt cgaccatccc aagtattaca acaaagccct tggctcaaag gcaactacta
    26281 tcattatcaa caaaaaagtt gattgcccga agggtaagtc gctgattttt tctgaggacc
    26341 cttttgcctg ctatgtaaaa ctggcaaaat tttactcccc gtttgtacca gcctcctcca
    26401 tgctcagcga ttcagccgta atcggcgggg gaacggtcat tcaaccgggc gcatttgtgg
    26461 ggaaccatgt tgtaatcggt aaaaactgca tcattcatcc cggcgtttcc atttatgacg
    26521 gtacccggat cggcgataac gtaatcattc atgccaacag tgtcctcggc agcgacgcct
    26581 actattttca gcgcaagccg gagggctatc gtaaactggt atcctgtggc cgggtcatta
    26641 tcggcaataa tgtggaaatc ggcgcgcttt gttcgattga taaaggggtt tcgggcgata
    26701 ccattatcag tgacggcgtc aaattcgata accatgttca ggttgggcat gataccttta
    26761 tcggaaagaa ctgccttatc ggggcacata ctgctatcgc cggggttacg cgtattgaag
    26821 atgacgtaat catctgggcc agggtttccg taaacaagga tattgtgatc gggaaaggag
    26881 ctgttatcct tgctacatcg ggagttgata aatcactcga aggcggaaga acctattatg
    26941 gcgttccggc tgatgaagca tttaagaaat ggcgtgaagt ggcagcaatc cgtaaattgc
    27001 cggggttgtt cagaaaattg cagtagatcg tagatattta cgagccttct tttcaaaaaa
    27061 gagggctttt ttattccccg gacgacggct cctgtaattg cggggcgccg gaggaagatt
    27121 gtttatcgtc ggtattcttt gcgttccggc aatgatatac atccagggca accaggatgg
    27181 caacgaaccc ccagaagggg acagaagcct tatcggtatc aaggaagtta ttcatgatgc
    27241 catgaatgta ataagtaata aggccaaggg taacagataa ggaaagcagg cgtacccttc
    27301 ggtcgggagc gtcgcggtaa actttcaacc cggtgtatat ggcggtgatc attatggcca
    27361 gcacgataag aagcccgggg aatccggttt cggccagagg cccgatgtat tcgctgtgcg
    27421 cattgccgag gtcgccgagg ttggtgctga taatggtctt ttcacgtgag ctctgaaaag
    27481 gtgcatattc aaactggtat gtgcccgggc cccagccgaa taaaggcctt tcgtgataca
    27541 tgcgcaaagc cgactgccac cggttgatgc gttccaggtt cgatgcatcg gacgatatat
    27601 tggaaatgga ttggatatgt tcaatcacat tggcagatga atcctgtttg tttttctcca
    27661 gtttatcgag gatctcgaat ttaaacgtga agaaaatggc gaaaatcccg accacggtta
    27721 tggcaatcca tttgaattta atgcgcagta caataagaat taatattgcg gctgctaatg
    27781 cacagctaac ccacgcagcc cggctgaatg acaggtacaa tgccataaac aggattgctg
    27841 cggcaatgct ggcaaaaatc cgtatcctta cagaggatga cttatcaaaa atatatccga
    27901 cgagcaaagg aatgaaaagc gaaaggatcg cgccataggc ggtatggtca ttgtaaaatg
    27961 gctgcatgac ccaatggcct gctttttcac taaaaccata ctgcttgtgt tcgaaggtgg
    28021 taatcgctat cacaataata agggggataa cataagccca gccaaatgat ctggcttttt
    28081 taacatccct gaataaaaat gcaccgagat aaaacatggg gattacaaac cataacctgg
    28141 cagcaaaata tttgaaggaa actatcgggc gcacgctcgt acaggaggtg ataaacatcc
    28201 agataaggct gataaagatg acaatagata caggatgcct gagtatcttt ttgtccggat
    28261 atccttcaat aatcactttg agggcaaaaa ccagaagaat tccgatcagc aagggttctg
    28321 acggcaacga tatgctgatg ccgaggtcat aattccctac gttaacggca agaggcgttg
    28381 ataagacggc aaggtagaac gctttttcga aatgaaagac atacaggaga atcagcatga
    28441 tggccagggg gatgaagata ccccagtaat actccttttt taccaccagg tatgtgttca
    28501 acaggatgaa caatgccgtg ataatgtata caatcgatat tttgatccgg tcggtcaaag
    28561 gggtgctgtt atgccgttat taaaagattg cttcgggaga attttactgt tcagtcggtt
    28621 tggacttctt tttctcaatg atcgaaattg tgagcaggct tagcaacaac gcgccgccaa
    28681 cgaaatagag catgatcagc acccgcttcg ggtacgattt tttatcagca atctgaggag
    28741 gcgtaaccac attcagaaag gtgaattctt tactggtatc gaaaacggca agatcatatt
    28801 cctgcataat cagatcatat tcattggcca gtccgtacat acggtatgcc aggtaaagca
    28861 aatcgccgtc cgcgtttttt attcccgtcc ggacgggact tggtttccgg ttgtcgttta
    28921 tcacgcccat agtttcggcg cttttcgaaa ggaaatcgtt atttccctgg tgggcattca
    28981 gcagggactt cgtggaatca atttcccgtt gttttacagc cagggcgctt ttgtaaccat
    29041 ccactacttc aatatatttc tgtcggtgtg cgtccctgat tttcatattg gtataataga
    29101 tgatggcatt caccatatcg cgggcaacca ccggatcgcg gtcaaacacg gtgatttcaa
    29161 cgctttcgta tggcgtctta gctactttta cgaatttgtc gtacaggtac gacatcgtag
    29221 aagtgaaata ctcgtatgat gaatcgatgc catagtgttt cggaagatca aatttcctca
    29281 tgacactatc ttttacatcc tttgaattga tccattgcag catctgttct gtttcacttt
    29341 catcagagta cggagcaata ttcgacggat aagccacggc aaccgattta aaccggggcg
    29401 ttataacgag cgggctcgat acgatcaacg aaacaagggc tgccagtatg gataatatca
    29461 ggaggtgcca tttccatttg gcaaggattt tcagcaggtt gttgttgtca aaaaaatttt
    29521 ccatgcgttt atgcctgttt gttctggttt ttaaattaaa gcaaaagagt tgtataaggt
    29581 cacttttctg atggctccct taattttttt acaggaaatg atttcagttt ctgcagttca
    29641 ttcccccatt gttcaacagc cgcaatagcc atggctgtga tcagaaaagt cgacaggatg
    29701 ctggtgagga ctattaacca ccggacggga taagcttttt tttcggcttt ataagcattt
    29761 tgaacaataa atttctgggg aatgctctta tgggcatcta tcttggcttc atcatagcgg
    29821 gtttttacaa aattgagctg ttcctgttcg agttcaagcg cgtcgcgtaa ggaaacgtaa
    29881 gctcccccgt attgtgaaag tatatccagt tttttttcaa gcgcttttac gccggttttg
    29941 ttgccccggg ctatttctac agcaagctgc tgattgatca tctcagcctg cgtttcataa
    30001 tcatgaaccc cttttttacg gatatccgtc aacgaatctt cttttttcct gacctcctgc
    30061 tgtaaacggt tgtattcttc ttcaacaatc cggtaaccct gcatggcacg ttctttttgc
    30121 atgtcatttt taaccgtatc aaagagcgag gcaattgaat tggcaatatc ggccgccatc
    30181 tgaggatccc tgtccagtac ggataccttt acggccatgt attcagtgcg tttgaaggta
    30241 atattgtttt cgtattgttt atagagcctg gtgttgaaga aacgggagga tggtttgatt
    30301 ccgtaatgat cggccagcct gaacttcgat atgattttgt cgcgcaacaa gccggagttc
    30361 aacacttgca gcatctgttc cgtttgctcg tcttcaccga attcgagaat gtccttgtca
    30421 acattggaat tatcgttgag caacgctttc gaaatggaat tgctcgaagc agggtacatg
    30481 atcacggtgg aacggtagcg gggcgtaata aaaacgggag atgaaaaaat aagcgaaagc
    30541 aatgctgcgg caacgctcgt aattataaac acctttctcc atttaagtat aaatccgatt
    30601 acactcgggg gcttggtatc agttgaaggg gtggagacag acatgctttt gccggacagt
    30661 aataaagtcg gacaaaatta gaataaatta tcgtatctgc aagaaaggct aaatgatttg
    30721 atcaccgatt gatggatgat tgccgttcag caagaacagg aaccctgtta tcggttccgg
    30781 atgagttcga aaagcgaccg gaaattgaaa agtttaagcg caatagccag cagaagagaa
    30841 aaggccatca tcatgaggaa ccgggtttcc caggctaagg aaaggctctt tgtcccgatg
    30901 ttgatggcaa taactccggc cacgtaaacg ggcagcgtga tcaggaattt cttgttgata
    30961 tgaaatttaa actgacgctg tacgatgatg atctgaatga tcccggtgac aaactgggtc
    31021 accaggctag cataggccga tccgatggcg aagtacttcg gaatcagcca gaaattcagc
    31081 ccgatattaa tgatcatgcc caggaatgcc gtgatattta acgccttcat actgccgttt
    31141 gccgtgagca gcgtgccaaa gatataggtg gtggcgatag gtataaaccc gatgataagc
    31201 agtctgaaaa tatttgagga ttcggttaca tggcttacat aaaggaggtc catcagttca
    31261 aagctgtaga aatacgaacc tgccgacacg atgatggatg ccgtaaaaag cagggtaaac
    31321 gagagtttca ccagccattc taccgaaagt ttttctttaa tcagctttgc aaacataggt
    31381 aacaggatga cggagaacag ataagcgatc atattgaccg catcaagcaa gcggtaagcc
    31441 gaggcataaa taccggcctg catgtcgccg ttatcgggaa gcagcctttc gagcataacg
    31501 gagtcgatgc ggttatagga tgccatcagc aacacaagca ccgcgtaagg gaaactctgt
    31561 ttgatgatca tccgggcaaa aggtatgctg agctgcaatt ttttaaaagc cgattttctt
    31621 accacgatac cgaaagcaat tactgcggtg atcagatagg caatggtttg ggcatagacg
    31681 aaccattcaa tccggaatgg ctgatcggtg acgcggcccc agagtaaaac gctgcaaatc
    31741 agtatcatga tgagcctgtc gagaaccgaa atcacgctgt cggtcttaaa atgcagtatt
    31801 cccgatatgt tcgatcgcag gtagagtata aatgaggtga gaaactggtt gaaacccagt
    31861 atcatcagga attgcatctg ccggggctta tatccgatga taaggccgac tccgagcgtg
    31921 accacgaaga acaggatgcc aagaagaagc ttgaacgaaa caatgcctga aaaatgcttg
    31981 ttgagcaact ggctgttctg cgctatattc ctgttgttga agtttgtaat cccgaaatcg
    32041 aggagaatat tgaaaatgaa ggtgaaattg agcagggaag cgtaaaatcc gtattcctcg
    32101 ggaccggtaa tattctgtac agtgcggtcg attcccagta tccagaaagg cttcaccaag
    32161 aagttcagga agagtaaaag ccccaggttg gtcagaaact tacggcgcat gtgctaaagc
    32221 cctgtaatga cagggcaaat tgttattgga attgaattgc tcaaaattat gaaattttcc
    32281 aaatgcaaaa agcaaaaggg agcagccgat ttatagttgt ctgtggcttc ggttaatccg
    32341 ggccggataa ataatttgtg aggaatggcg ttgcagtacc aggcgttttc cggatagaat
    32401 caataccttt gtaaaaaaat cggtttgaaa atagcagtaa atacacgatt gctgatcagc
    32461 aaccgcattg agggaattgg ccggttttcg gttgaaacgc ttagccggat ggcaagggcg
    32521 caccccgagc atgagttctg ttttatcttt gaccgcccct atgataaagc tttcgtgttt
    32581 tccgacaatg tgtatcctta tgttattcct ccccaggcgc ggcacccttt cctctttctg
    32641 atgtggttcg aactctcggt gcctctcttg ttcagaagaa tcaaacccga tttgttcgtt
    32701 tcgcccgacg gctatctttc cctgaatacc cgcgttccat cggttgttgt gatgcacgac
    32761 ctcaatttcg aacattttcc acaggatctg ccgtggctca tcagccgtta ctatcgcagc
    32821 atgtttcccc ggtttgcccg gaaagccaaa cggatagcaa cggtatcgga gtactcgaaa
    32881 caggatatcg ctgagcggta ttccatttca catgagaaaa tagatgtggt ttataacggt
    32941 gtttcgagtc attttcaccc gttaagtgaa gcagaaaaag aaacagtccg acggagtttt
    33001 tcgtccaata agccctattt cctgtttgtc ggagcgctaa atccgagaaa aaaccttaaa
    33061 aacctctttc tcgcttttga taagttcaaa gaaacggacg acctggaaat caagctcatc
    33121 gttgccggcg cccgtatgtg gtggaacgat gacatgcggt ttacctatga aaatatgcac
    33181 caccggcatg atgttatctt tacgggcagg gttaccgatg cggaacttgt caggctggta
    33241 ggctcagcgt tgtcactgac gtatgtttca ggttttgaag ggttcggcat tccgatactc
    33301 gaagcttttc aatgcaaaac gccggtaatc acctccaatg taacatcaat gcctgaggtg
    33361 gcaggcgatg cagccctgct ggtcgacccg ttttcggtag aagaaatctc ggaagcaatg
    33421 gtaaggatgg cgagagaacc ggaattgcgc cggcagttaa ttgagaaagg aacggaacaa
    33481 tgcacgaaat tttcgtggga cagatcggcg gaattgctct ggaactgtat agagaaaact
    33541 attagataat taagatgact gtaagagaac tagtgaagcg gattttgaag tactcgccaa
    33601 ttgcacttac taaaaatcaa caatacgaca ggcaaacaaa aagagtaatc aggaaggttt
    33661 gtcaacacga taccaatacg gttgatgtag gttgccacaa aggtgaaatt ctggacatat
    33721 tgttagaaca tgctcccgac ggcgttcatt acggctttga gcctattccc ggtttatttc
    33781 attccctgga ggcaaaatat tccggatttg caaattgcca tattttagat tttgccctca
    33841 gcaatgtcaa aggatcatcg tcctttaatc ttgtactgga cgatcccgcc ttaagcggtt
    33901 tgaagaagcg cgcatacaac cccggctacc gttcgcaaac cattgaggtt aaaacggagc
    33961 ggcttgacga tatcattccg gctacaacaa aagtggggtt gatcaaaatt gatgttgagg
    34021 gaggtgaact gcaggtttta atgggcgctt cggagctgat ctgtcgcagt aaacctgtta
    34081 ttatattcga acatggccag ggcgcctctg aattttatgg cacccggcct gaacatgttt
    34141 ttgatttact tgaaaaatgc ggtcttaaag tttcgctcat gagccgctgg ctgaaacagg
    34201 gacctgcctt gccgaaagcc gggtttatag aacaatatca aaagaaggtc aattattatt
    34261 ttattgcata tccggattaa taccgggccg cctttcatta aacgatattc acttccatct
    34321 caagttttac tccgaattta tcgagtaccg aatcctgaat ttttcccgcc agggtgatga
    34381 tgtcgatacc cgtggcattt ccgtaattta ccagcacaag cgcctgtttg ggatgaacgc
    34441 cggcatcgcc ttcacgatag cctttccatc cgcatgtttc tatcagccag ccggcggcaa
    34501 gtttcattga tccgtcaggt tggtgatata ccgggatgcc tggatatcct gcgatcaaag
    34561 tttccgcttt tccggaactg acaacgggat ttttaaagaa acttccggcg ttgccggtca
    34621 cggcagggtc ggggagtttg ttgcggcgga tccggcatat agcctcgctt acatcggcaa
    34681 ccgcaggttc tttaatattc atggcttcaa gttcctgcct gatagcgcca taatcgagcc
    34741 gtaaaacagg agttttgctc aggcagaagg taactgccag tatcagcatt tttcctttca
    34801 gcgccctttt aaagatactg tcgcgatacc cgaaccggca ttcatcttta gtaaatacct
    34861 tttctgatcc tgtttcaaga tctattgccc gtaacgaatg gaaataatcc ttcagctcaa
    34921 cgccatatgc gcctatgttt tgcaccggtc cggccccgac atttcccgga acaagcgaaa
    34981 gattttcaag tccgccgagc ccctgtttaa tggcccagga aaccaggtca tgccaaactt
    35041 cacctgcagc agcttcaacc agggcgctgt cgggagcgct ttcaaccatc ctgatccccc
    35101 ggttagccac atggatgaca aggccgtcga aatcgccggt aaacaggata ttgcttccgc
    35161 caccgatgac caaatgcagt ttatccctta acactccgct ttcaacaagc tgaacggctt
    35221 cttcctggtt ctttatttca acaaattgcc tggcagtggc ttcgatgccg aatgttgtat
    35281 agggctttaa cccgacattt tccagtatat tgagcatttc ccggataata aaaaggtgaa
    35341 cgatcagttt aggttaaagc aaaagtaatc cattcctcag gaattgtccc cggttgagga
    35401 atattttacc tgaatagact attttcgcat tataaacatg agcagcaaga aagccattat
    35461 ttccgttatt aatgacctgg ttaccgacca gcgggtggat aaaactgccc gggtgttact
    35521 ggaactcggt ttcgaagtga ttatggtggg gcggcggaaa cgcgacagca tgcctatgcc
    35581 cctaaagcct tataaaacaa agcggatgaa actgttatgg gaaaaagggc cgttgtttta
    35641 tgccgaatac aatacaaggc tcttcttgta cctgctcact caccgtgcat ctttactgat
    35701 gagtaacgat cttgatacct tgctgcccaa ttttctggtg ggccggtttt tccgggcgcc
    35761 cctggtatac gacagccatg aatatttcac cgagactccc gaactgaccg gcaggccgct
    35821 tgtgcagagc gtctggaaat tcatcgaaag aatgatcgtt ccgaaactga aagattgcat
    35881 taccgtgaat gagtccattg ccgggctttt ttacaatctg tatggcatca agttcaaggt
    35941 ggtaaggaat atacccgcga agaaaaatca tgcctcagtc ctttcgcgcg aagatcttgg
    36001 cctgccggtt gacaaaaaga ttatcctgtt acagggcgcc ggtattaaca ttcagcgtgg
    36061 ggccgaagaa gcagttgaag ccatgcgata ccttgatggc gcaatcctgg tgattgtcgg
    36121 cgggggcgat gtgctgcctt tgctgaagaa gcaggtcgcg gaatcgggac tgcaatcaag
    36181 cgtgatgttt gtgcccagac aggttcctga aaagctgttt tcctatacgg taaacgcgga
    36241 tattggcctc gctatcgaca aggatactaa tataaactac cggttcagcc ttcccaataa
    36301 attatttgat tatattcagg caggaatccc ggtattggtt tccccgttgc cggaaatcaa
    36361 acgcatcgtg gacgaatata acatcggtga ttttattgag agtcatgacc cgcaacacat
    36421 tgccggaaag atgtcggcca tgctttccga ccaggaccgg ataaatacct ggaaaaccaa
    36481 cctgaaagtt gctgccgaaa aactcaactg ggaaaacgaa aagcaggtgc tgaccgaaat
    36541 ctattcacaa tatgcctgac cgtcatttga atattgtttc atttgccatc ccttatccgc
    36601 ctaattacgg cggtgtgatt gatgtttacc ataagttggt ggcgctgaaa aatttaggtg
    36661 taaaagttca cctccattgt ttccgctacg atcgccagcc ggctcctgag ctggaaaata
    36721 tttgcgagtc cattcattac tatccgcgta agacaggctt tttttcggcg ctgagaggta
    36781 aaccatacat cgtcataagc cgcaggagcg aagaattgct gaagaatctg tgtttaaacg
    36841 attatcctgt ccttttcgaa gggctgcata cctgtttgta tctgccgcat gaggcgctaa
    36901 ggggccggtc cctgatttat cgcgaaagca atattgagca tgattattac cgtcacctgg
    36961 caaatgccga aaagaaccct tttgtaaagg ccttcatgta tatcgaagct tttaagctgg
    37021 catctttcca acgggtttta aaacatgcat cggcgatgct ggctgtttcg cagagtgatg
    37081 catcttatct ccactcccgg tttccatccg gaaaagtgat atatctcccg agttttcacg
    37141 gtaattatac ggtaaaatcg aaagaaggcc ggggcgcata tgccctgtat cacgggaacc
    37201 tcactgtagc tgaaaatgtt aaagccgctg aatatctgat caaagaagta ttcaacaacc
    37261 tggatatacc gttaaaggtg gcaggactga atccttccgg gtcgttaaag aaactggctg
    37321 ataagtacgg gaatgtctcc ctgattgcca gtcccgacga aaagcggatg aatgaactcg
    37381 tgagtgaagc ccatgtaaat atcctggtaa cttttcaacc gacaggcctc aaactgaaat
    37441 tgctgaatac attatacaaa ggccgtttcg ttctggtaaa ccctgatatg ctggctggtt
    37501 ccgggttgga tgttttatgt gaaacggcaa atgatacaat atccctgaaa gcttctttgc
    37561 tgcagctctt tactgaaact ttcgatgccg ggaaaatcat acagcgccgg gattatctgc
    37621 agaagcatta ttcggatgaa gccaatgcaa aaaaactggt tgaagttgtt ttcggcggcg
    37681 accaggacta agagagaggt aatccttttt ggatagacca tccatatttt ctccctgagc
    37741 ggttactctg aattcaaaat tttaaaaaaa ccgatcagca ccggtcgcgg aaccggatgc
    37801 tgaatcggag atttaaacag gctaaatgcc tgaaaaagtt acatgtgtaa ctggcggata
    37861 tgtaaaatta tttggcaaac aggatatatc cgtcgacgct ccagcaagga acaatcccat
    37921 taatggtaca gttaccggtt gcaccttcaa atctgccggt gccgccggtt aactcaacat
    37981 ccccggtaat tatcttggtt gggggaattg taacatcaat ctgtatgtaa gaaatatagg
    38041 aaagtttatc atggttggct gcggtgagaa ttccatcgta ggttgcggca ataaccacct
    38101 tgccctgagg caacaactct gtcatcaaat aggcatcata tccggtcata ttgctttctt
    38161 ccttgatcaa ctggcctata tgggttccat gtccgctgag caaggatccg acagataaat
    38221 ccagactggg aatgatggtg tctcccaact cgttcacaag gggtgttccc tccacgtgca
    38281 aacgattggt atcgggggtc atgcagatgt caattttcat aggaaccggt actttcttca
    38341 ggttctttac cccagggctt acttcttcgt ccatcggggc tttggtgcaa ctgaaaagca
    38401 ttaccaaagc cacagctaaa aggtaaatca ggtttttcat gcttgcagga tttatggcga
    38461 ttgatgataa ggttaacctg ttaaagttat acaggtattc gccggattgt tttgctgtga
    38521 gtttgaatag ttttgctgtg gatataaaac aaccggcatg atattatcct ggtgaatatg
    38581 aggggacaga ttttttatgc agataatcag ggcgcatgcc cggaaggagg gagcaggttt
    38641 ttctttaact cagggtttcc ggaattttcc gggtgatttt ccgaattgcc gggtaaactc
    38701 gcggctgaag tgcgaaagat ttttaaatcc aaccgcaaag gtcacttccg ttacgttcag
    38761 gctggtagtc agcagcaatt cttttgcttt ttgaagcctt aaaaacttga aatagtcagc
    38821 tatcgtcttg ttgctcaatg atttgaattt ttggtaaagc tgtccccggc tgacggccag
    38881 ttcgttgcag agttgtgtaa tcccgaattc ttcattttcc aggttcgctt ccagcacctg
    38941 ccggaccctg atcatgaatt catcttcttt tttcaggtaa atgtcggatg ttgccggaat
    39001 tttctcaatc gaagagtatc tttcatggag ttttttacgc tgatcaacca ggtttttgag
    39061 ttggatgaac agttccttct catcgaaagg cttggccagg taagcgtcag cccccctttc
    39121 aagacctgca agccgtgaat caatatcggc ttttgccgtt aacatcacta ccggaatatg
    39181 gctggtgcgg atatcgtttt taagtttttc cagcatggcg attccatcca gaacaggcat
    39241 catgacatcg ctcaggataa tttccggtac gaggttcagc gccttttcaa agccggtact
    39301 gccgttttta gccacttcta catgatattt tttttgaagg atagcccgga gatattctac
    39361 caggtcaagg ttgtcctcaa caatcagcag caaaggggcc ccgtcctgca tgacggcctc
    39421 ttcctgcacc acctcaggag aggcgtccct gaaaattaaa ctagcggacc aatccccatt
    39481 agcctgttgc cttgtttcct ctgtttcatc tgcctcctgc cggatatcag tttccggagc
    39541 gtttcgtgtg accggcagaa aaacggaaaa ttccgttcca atgcccggta tgctggttac
    39601 agaaattgtt cccttcagga gttccacggc gtctttgacc acggcaagtc caagccccga
    39661 acctcccggc gaattattgt tttcaacctg gtaaaaacgg tcgaataaat gatccatatc
    39721 ttctttcctg ataccgatgc cggtatcttt gacccggata gcaaattgtt ctgcatcctc
    39781 atgcccattg gcttccacct taaccctgcc tccttcggca gtgaacttca gggcgttgga
    39841 aatcaggttc gacatgatat gcattagttt tttttcgtca aaatccatgt taaaggatgc
    39901 ctcttccggt tcaaaagtta aatcaatttt tcgggtgatg gcttccggtt taaactgctc
    39961 aaccaaggaa tacaaatagt tgataatatc tttccggcag agggatatgg tgacagcgcc
    40021 ggtttcgatc cgggccatgc aaagcatctg gtttaccaga tgaagcagga tatcgctgtt
    40081 ggccctgatt tttagtaaac cggtttccat ccattgttcc ggctgtgttc tgatcagatc
    40141 ggtcatcccc tgaataacgg tcagcggagt gcgaaattca tgggtaatgt tggtatacaa
    40201 cctgtttttc atttcatgga tctctttcag atggttggtt tcaataacat ccagtttccg
    40261 ctgttgccgg ataaaatcca gtacaaaaat gagcgaagcc ccggttaaaa atatagcatt
    40321 gagcatatat agaacagtat tttcaaaagg cttaatcagt tgcgacggat acaaccaggg
    40381 ctttaatacg accagaagca ctaccagaac cagataaatg gaaaacaaag taattgaata
    40441 ccatgccttt tgccgcggaa ccgtggaaag cacattggcg attcctacaa atatcagccc
    40501 tccggaagcc ggtatgcctc ccattttcag gatacaatag aacgtcgcaa ggtttaaagc
    40561 cagtccatgg gataaaccga taaaaaggaa atgcctgggg aagatgagtt gtaatgaaaa
    40621 ggagagggcc agaagggtca tcagggtcag accatacccg atgataatgc tgagctcggg
    40681 ggcaaatatc aggcaaccgc ttacgagaat caggatatag gtaaaagtga agaagatgtt
    40741 tttcatcgcc gacaggttgc cctgaagttg cttgctggtg cagcccgggt atgaaagcct
    40801 tgttaacatc aatgattcga tcttttgcag tctctcaata acaggattca tagctgtatt
    40861 gtaataaaaa acatatgaat atttaccata tacaatttac gacaaattat gctgtaaacg
    40921 atcagtgggc taagatttat acatggcccg attctctgaa tattagacct ttcttgcaaa
    40981 tttcttcttt tatcactatc ttagggtgta aatattgcag cgtctgtcag tcggttgcat
    41041 ctcgtcgtct ccggttttaa aatctcatct cattatccgg taaaaagcca tggttagcca
    41101 ataaccagga ttcttctgcc cggaggaatc tgaaattata tccgctcaat tttttcaatt
    41161 ctaaccttaa accgatgaaa atgaaaaagt ttgtcttttt actgatcttt attccggctt
    41221 ttgctttcgg gcagaagccg gctgccacgg caacagccgc agggaaggac ctgaaagaat
    41281 ctgctgaagc atttgtcacc cgttttattc aatgctggga tgataaaaac tgggatcaga
    41341 tttttaattc gattgaggaa accgggacct attattctgc cgctgaaaca acccccttgc
    41401 ctgaaatggc aaaatcaacc gtcaattact ataagtacaa agttacggat gccaggacta
    41461 ccgtgaattt cgtttcggcc gacgtatatg gtcaaaacac agcgatggtc accgtgaggt
    41521 acctggagtc gctgaaagcg gaaggccgtg acagagtttt ggattacctt gacattctgg
    41581 tccttgaaat gaaagaagga aactggaaaa tcagcaggtg gtactcgcaa tgtttctatc
    41641 cggtgatcgt caatcccgct attgatggga aatggcaaaa aaatgtcgct gaacctgtct
    41701 ggcgttattc cggcgccctg taccaatttt ataacttgaa ctgctatttg atagaagata
    41761 ataaaaagaa cggaaccagc ccggcgcaac tgggaaagtg gatgggcgaa cgcttcgcaa
    41821 aaacctggaa ccagtcaaag gggtttgaag ggctcgtatc aggatatttc tggctgatgc
    41881 agtccatctc gccctatgtg gagtttactg aacgaaatga aaacattgcc cggattaaat
    41941 tcaagtttta taatccggat cctgaagact ggaatgtcac tgaaaatgaa atgcttgaat
    42001 gtttcaggaa cgttttgatt gaaattgccg ctaatatggg aggaacctgt tccatagctc
    42061 ctgatggcaa attttacatg atgacgctgc aagcggttcc tgggtggctg cctgacaagg
    42121 attccaaaat cgtgaaggaa tcgctggccc ggggcgatac tgttaaagcg gacctcgtga
    42181 aggcgaattt tctgacaagt cagggaaaaa cagaagaggc ttcgaaaatt ttcacgagga
    42241 tcatgaaaat caggccgcac aacaaagaag ccgtgcaggg atggcttttg gcaaacatga
    42301 aaaagagccc tacaggggag gaggatgcta taaagtcatt ggaagagctg gaaaagctgt
    42361 atcctgagaa ttccggagtt atattttgga agtcttttat cgaagctgaa tacgggcata
    42421 atgaagaggc gctgaaggat tgcaacaggc ttattaaaat ccaacccgac agcgcgataa
    42481 attacatcgg caaaggacag gtgctgtatg ccatggaaag atacaaagaa gcttttaaag
    42541 cattcgacaa ggcaacttcg ctcaatcccg aacgatcgga cgtgtggggg atgaaagggg
    42601 ctgcgctggc caggctgggc aggtatgacg aagccattgc ttcgatgaac agagggctga
    42661 aacaggatcc ggataatccc aataatatat ataaccgcgc ctgtatatac agcctgaaag
    42721 gggacaaagc cagggccctt gccgatctga aaacggccat ctctctgaat cctgaattca
    42781 gggaatactc ccggaaggat aaagactttg agcgccttta tgacgatgag gattttaaaa
    42841 agctgaccga atagatattc cctttaaagg aaatgggaca aaatcggcca gtctgaatgg
    42901 aagacagtcc ggatttaggt tcatacgggg atctttttac agccatcagc ttcagatgag
    42961 ttctgatagc aggttaacgg tatgcggctt tatcaatgtc ggacagtgat ttcagcttgt
    43021 gcggcggatc gaggtattta tccaggaaag catagatctt gagccggatt tgttttgcaa
    43081 tgcgggtgtc gatgcggtcg aaagaatggc cgccttccac atcctggaaa atctcgtatt
    43141 cgaaatgttt tccttcggcc ttcaacgcct ggatcaggtg ttcaacctcc agcacgttga
    43201 catcgtcgtc gttggtattg gtatggatga gcaaaggagt ctgaagcttc caggcgttcc
    43261 agacaggcga acgtctgcgg tattctttga tatcctctct tgcgtccttc ccgatatggt
    43321 aggttgccga aaacagcttc cggtactcgt cgtccgaata ccccatgcgg gctaccaggt
    43381 cgctgacggg gactcctgcg aagcagacct gatatttttc agggtgatcg aacacgcaca
    43441 tcagggcaat catcccgcca tggctccagc ctatgatgcc gacccgtgtg ctgtccacca
    43501 tttcataatt ccttaccata taatcgcggc tggcgttcac gtcgtcgttt tcaagcccgc
    43561 cgtaatcgat cttttcatag aagtcctttc cgtaaccggt gcttccccgg tattccgggg
    43621 ctatcacgat atattgctgc gccatgagct cccggatgat atgggcatgg taggtggtaa
    43681 aatccgcgtg aacgcccccg tgcggaagca ccagcagcgg gtatttttta tcggctttta
    43741 tatctttcgg gataaagatg tagctgtaaa acttcaccgg atttcgggcg cccatggcgg
    43801 tagggttttt tacctgcttt tccggtaccg gcgggcccgt gatgtatact ttatcgataa
    43861 aggcaacgtc accaacccgg ttgtaccagt atatatcatc gaccgctttc tgcaggctgg
    43921 caatatcata tcccagcgat tctattttgg cgttgatttc tttcagcgtg ctgtcgtcgt
    43981 tctgggctgt tcccggaata acaagcgcga aaagcaaaag aagcaggaaa agcggtcttt
    44041 tcatggcaat atcggtttaa taagcaaaga tagaagaata acggaaaggc tttatgccga
    44101 tgaaatcagt aaatgaatga agttgttctt cagtttgtgg ttgttgccaa ccgtcgtata
    44161 aacctgcata accgatggga tggcggtgac gtttgaggga aaagagatat ggattctggc
    44221 ttcgactaat cttagccagc ggggtttgct tcgctgagtt acagatatgt accagtcggg
    44281 gtataaatga ggcactcgtc aggaaacgag cgccaaaaaa ggagaagaat cccgattttg
    44341 aaagctcagg ataagaagta aaaattacaa atcgctattc tttaatacaa cggactgaat
    44401 aaccgtagtt tttattcgta caggctagtt ccgccgaact tttatcttta tatagattca
    44461 ggaaccatgc aaaattacaa gtttcgctgg tgctactcca ataattacca tccaagccaa
    44521 tttcaacgta gccgtttaca ttacaaccac cgggtaagcc tgaaaaaccg ctttcatttg
    44581 tcgctccggt atttggagaa gcccaaactg aaccggttgc ttttaacttt ccaccagcta
    44641 caccttctcc tcccaaaaat gctattaaag tttcccaatc agattttgtc ggaatgcgcc
    44701 agcctttggg agccagcttg ccagtgttaa ctgcctgcca attatagagc ctgccatagg
    44761 ttgaaacata agagtccaaa ttgttataat tacaatatgc tcctgaagta agggagttcc
    44821 acgtgtttgt gttggttata ttatcaatag aagtattatc attatacttt gtgactttta
    44881 gattttctac catccagact tgagttccta tagttactga atgataaaca tttccatcaa
    44941 tatctttgat tgtactgctc ccgctatctt cgtctttatt acaacttgaa aatagcgcca
    45001 caattactcc caatgtcagg gatgtagtca gcagaaggaa agccttggtt ttcatttttt
    45061 ttagtttttt aatatgtgtt acaatcgttg gagctttgct atgattatag tattattgaa
    45121 tttaagctta tcatataata atccccggta accgaagtat caatgaacct gtaatcagat
    45181 tatctgcttc caaatatacg aaaggatcag tggatataga agaaatatca aaaaaaacgg
    45241 aatgtatgac atgttgctcg cgtcttctga catgtgcgct gaatttttca gttcatatgc
    45301 agttgccata aactcaatac aattacatcc ggcaccgatt tcttacctga ataatccaat
    45361 gctgaaccat attcatactt ccctcgctca tacgcgcttg cagcgggcaa aaacccgtgt
    45421 tgaagaactc tttactgaaa tacaaattta cccatccccc tctcactctt atccgcagcc
    45481 gattgcaaat ggaaaaaata cactcccgtc ccgatttcgg ttttaaatat atagatatca
    45541 tcgtcagtag attctattac tttcacttca ttccccagta aatcataaac cgataatctc
    45601 ctcttttctc tttttgcatt acgaaattca acctttacat aatcactgct gggattagga
    45661 tacgctgtta ttttgctcaa agtcaggtta tcatttgggt tgcaggatgt aattgttcct
    45721 acctgattca gttcaatcat gtcaaatctg gccgataaca tgcaaaaatc acacatgtca
    45781 gctgttagcc gaacggcaat cgtatcggtt tcttcggtgg taattgtatc tgttaaagtg
    45841 taaaatctaa tagttctcca ttctatattt gcacttgcat gttttcagta atttggtgcc
    45901 gccaattcat cgttagctat tttatagatt ataattctcc ttaagtttgc atttcttatg
    45961 aggcttaata agcccctgtt agtaactcat tcaactagtc actgctctct atgtctaaat
    46021 tcattactgg aaaacaactc gaagatgccg tttatgacat tatttggaat gcatcaaata
    46081 gtctcctgat cgttagtcct tatatcaatc tcgatatata tttccgcaaa ttattcgaaa
    46141 aacacctgaa caatcctcgg ctccaaatca caattgtttt tggcaaaaac gaaaacaatg
    46201 tgaagagaag tcttagtgcc gaagattttg atttcttcaa gaaattcctt aatgttagtg
    46261 tagtgtactc acctcaattg catggaaagt attatggtaa tgaaacccaa ggtgtgatta
    46321 cttccatcaa tctctacgat ttttctttca agaataacgt agaatttggt gtttttcatg
    46381 agactaactt gctcaatagt atcaccaaat cagttgatca ggaagcatgg ctggcgtgtt
    46441 gggagattgc gaacaatgct gaagccgttt ttatcaaacg tcccgttttt gagaagaaac
    46501 tcatttctat tcttggtaaa aattacgtca aatctgatat tcttttggat aataccgaga
    46561 gatactataa gtcatcatta aaatacaaat cgcttccggt caaaagatta tcagattacc
    46621 ctgacgaatt ggaactaggt tctttacctg acacccgccc ctcgcgtgag gaagtagaat
    46681 cttttccaag atcagtttca atacgtgatt caaaacctaa aatgggctac tgcatccgta
    46741 cgggtcaaca aattcctttc gacccttccc acccattctg ctatgaagcc tatcaggaat
    46801 gggcaaaatg gaacaatccc aactacccgg aaaagttttg ccaccgcaca ggtaaaccat
    46861 cgaatggcaa gacttcaaaa agcaatcctg ttctttatta gcactgatta tctatcataa
    46921 tttcttcttt aggcttcccc ggacattggc cgtttaaaat catatgaata agtaaaactc
    46981 ttgattttca acagctataa acacccgttt cctgttgtac ttattctata c
//
LOCUS       DAOPYV010000015        46447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_637_length_46447_cov_12.670676, whole genome shotgun sequence.
ACCESSION   DAOPYV010000015 DAOPYV010000000
VERSION     DAOPYV010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 46447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46447
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_637_length_46447_cov_12.670676"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..2512
                     /gene="pheT"
                     /locus_tag="PKI35_03270"
     CDS             44..2512
                     /gene="pheT"
                     /locus_tag="PKI35_03270"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNX43433.1"
                     /translation="MKISCNWLNEYVRTDLDPVETARILTDCGLEVEAIEQYESVKGG
                     LKGVVIGHVAETRHHPNADKLTCCKVDVGGERLLDIVCGAPNVAAGQKVPVATLGTVI
                     YKGDDSFVIKAAKLRGEPSEGMICAEDELGLGNSHAGIMVLDAGAQVGTPASDYFGIF
                     TDTILEVAITPNRSDAASHFGVARDLAAAINNRERQHPSASFVKPLVDAFRSDNDNLH
                     IDVSIENPEACPRYSGVTLSGLTVKDSPQWLKDRLKSVGLRPINNIVDITNFVLMETG
                     QPLHAFDADKINGKKIVVKKQPAGTKFITLDETERSLGADDLMICNAAEAMCIAGVFG
                     GLKSGVTGETTSVFLESAYFDPVHIRKTSKLHGLKTDASFRFERGADPNITLYALKRA
                     ALLIKEIAGGNISSVIVDVYPEPVSNRILDVTFDGIENLIGKKIGKDAIRNILKSLEI
                     EIIAETDGDLTLSIPPFKVDVEREADVVEEVLRIYGYNNVAFNESVHASLSYNPKPDP
                     EKIMNNVADLLSANGFREIMTNSLTSSAYYEKSEVYPADRCVRIFNPLSRELEVMRQT
                     LLFGGLESVAHNLNRRQNDLKFYEFGNVYSYNPSESHEADVTARYSERKKLSVMITGK
                     RLSESWYNISQKADYHLLKSFVFLLLRKTGVPYWQWKISPADQRLFTEGEAFAVNGNN
                     ILSLGKLQPSIIKTFDIRQEVFFAEIDWALLLSLVKPGNSRQKDIPRFPEVRRDLALI
                     LDKGVKFSDIEALAYKSAGSTLKSINLFDVYEGEKISEGKKSYAISFTLLDESKTLTD
                     KEIEKLMDRLANDFTKELGAIIRK"
     gene            2545..4206
                     /locus_tag="PKI35_03275"
     CDS             2545..4206
                     /locus_tag="PKI35_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNX43434.1"
                     /translation="MMQAPITYSASPSGEKFPLPQKEEYATEFLNISKLAENARKEGK
                     EVVVVMGVGFVGAVMAAIIADTVDSQGKPSKFVIGCQRPSTRSYWKIPMLNRGESPVK
                     AEDPEVDRIIARCVNQKQTLIATFNSDCLELADVVVVDVQCDFSKRELGNMKTGEADM
                     AALEATLKTIGQKIQPHCLTLIETTVAPGTTEFVAWPILKKAFAKRKITSVPLLAHSF
                     ERVMPGREYVSSIRDFWRVCAGCDNEARKRVEKFLREVLNTKDFPLTVMDRPIESETT
                     KIVENSYRATILAYLNEWSIFAERNGVDLLKVIKAIKMRPTHSNIIFPGPGIGGYCLP
                     KDGGLGYWAYRHILGFDDGDDVFRITPTAIDINDTRALHVASLTRDALRNMARYIAGA
                     DVLLCGASYRQDVGDTRYSGSELIVRKLTEMGAEVHIHDPYVEHWYELESQDEYPAPG
                     HSWQRFFHNQDHLADTKVLKDLPEALNGIEALILAVPHTEYKNLKPDDVVKWAGQPLA
                     VIDCFGLLSDEDIRRYFELGCEVKALGRGHIQRIKKEVRRAAPKN"
     gene            4418..5647
                     /locus_tag="PKI35_03280"
     CDS             4418..5647
                     /locus_tag="PKI35_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNX43435.1"
                     /translation="MDIISIKQRFGIIGSAPGLDRAIDIARQVAPTDITVLITGESGV
                     GKEVFPQIIHQLSARKHGPYIAVNCGAIPEGTIDSELFGHEKGSFTGAHEARKGYFEV
                     VNGGTIFLDEAAELPLATQVRLLRVLEAGEFMRVGSSKVQKTDVRVVAATNVDIQAAI
                     NEGKFRQDLYYRLNTVPIAIPPLRERKDDILLLFRKFAVDFAEKYRMPPLELDDEAKH
                     MMLNFRWPGNVRQLRNLTEQISIIEHVRTITAHVLQKYLPDTANRELPILYTGSKPGG
                     EFSERELLYKVLFDMRRDINELKKLVGDTAGHDPVSAHLHSGVAGMNENMPFPGTDRQ
                     FHAAHPYVVENPDNEEELFSQSEVVDESLSIQEKEIDLIRRALEKHAGRRRNAAKELG
                     ISERTLYRKIKEYGINA"
     gene            5663..6193
                     /locus_tag="PKI35_03285"
     CDS             5663..6193
                     /locus_tag="PKI35_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNX43436.1"
                     /translation="MNYKTHFTSARFLMITMALAAINLLSGCGIYSFTGASISPDVKT
                     ISVKYFPNNAPLVQPTLSNTFTEALRDKFTNQTNLKLVKSGGDLNIEGEITGYSTEPV
                     AIQGNQQAALQRLKITISVRFTNKMDEKQNFETSFSRYEDYDASYSLSQVESGLIETI
                     TDALAQDVFNKAVVNW"
     gene            6211..6981
                     /locus_tag="PKI35_03290"
     CDS             6211..6981
                     /locus_tag="PKI35_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX43437.1"
                     /translation="MERRSFEKILADRSLLTGSTVEMITALTREYPYFSTAYLLVARN
                     LRSTDNESFTPALRVAAAYAGDRKRLKMLIESDEIKELPLAMQDIDLAGTMIESGTPA
                     APEKQASPETISTAPVHPESGEGKIPAGGVLIGLIRDSLSEIADERKENGNAVSAHPP
                     KEKETAGKSRFELIDKFISEEPRISPSKREFFSPEDKARQSIADHDDLVSETLAHIYE
                     KQGLSEKAIKIYEKLILLFPEKSSYFAARVEELKNNRK"
     gene            7012..7347
                     /gene="secG"
                     /locus_tag="PKI35_03295"
     CDS             7012..7347
                     /gene="secG"
                     /locus_tag="PKI35_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNX43438.1"
                     /translation="MTAYILISVLILIACVLLTLVVLVQNSKGGGLASNFSSSNQFMG
                     VRKTADFLEKTTWTLAVILLSLSLLSIFVIPRHRANQAQADTELRQNIDENAQPVQDY
                     QPNPANQGK"
     gene            7645..7923
                     /locus_tag="PKI35_03300"
     CDS             7645..7923
                     /locus_tag="PKI35_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNX43439.1"
                     /translation="MAQLKVKPLADRVLVEPAAAEQKTAGGIIIPDTAKEKPQRGTVV
                     AVGTGKKDEPLTVKEGDLVLYGKYSGTEISIDGKDYLIMRESDILAII"
     gene            7952..9592
                     /gene="groL"
                     /locus_tag="PKI35_03305"
     CDS             7952..9592
                     /gene="groL"
                     /locus_tag="PKI35_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941147.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNX43440.1"
                     /translation="MKAAKEIIFDTEARDRLKKGVDALSNAVKVTLGPKGRNVIIEKT
                     YGAPAVTKDGVTVAKEIELKDSVENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIIT
                     TGLKNVTAGANPMDLKRGIDKAVATVIESLKKQSKEVGDSLEKIEQVATVSANNDSTI
                     GKLIAEAMAKVKKEGVITIEEAKGIETTVKVVEGMQFDRGYISPYFVTDAEKMEAAYE
                     NPYILIHDKKISGMKDLLPILEKVVQTGRPMLIIAEDVDGEALATLVVNKIRGSLKIV
                     AVKAPGFGDRRKEMLQDIAILTGGTVISEEQGYRLEDADLSYLGQAEKVSIDKDNTTI
                     VSGKGEHDGIQARINQIKAQIETTTSDYDREKLQERLAKLAGGVAVIQVGAASEVEMK
                     ERKDRFDDALHATRAANEEGIIPGGGVAFLRALETLTTLKTENEDEEIGVNIVRRALE
                     EPLRQIVANAGLEGSIIVQKVKEGKGDFGFNARTETYENLLKTGVIDPTKVARVALEN
                     AASIAGMLLTTECVLAEIKEEKEPPMPNPGMGGMGGMY"
     gene            9744..12536
                     /locus_tag="PKI35_03310"
     CDS             9744..12536
                     /locus_tag="PKI35_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNX43441.1"
                     /translation="MSAKSYPEAAAAKIIAQDSSVTTDFINIFTNKQHHTMKNFSFRK
                     WTILSIVLLAIPSVTYSQKKLPYQDPALSAEARTKDLLGRMTPDEKFWQLFMIPGDLS
                     LGKQNLKSGIFGLEVDARGETKDAAGQMLTYGGKEDAGLYANKVNEIQRYFVNETRLG
                     IPVIPFAESLHGLGMGGSTSFPQSIGLAATWDTSLMRNVSRAIAREVRSRGIRQVLDP
                     VINIARDVRWGRVEETYGEDPFLTTMMGICYMDEFEKLGVITTPKHFAVNVGDGGRDS
                     YPIHYNERLLEEIYFPAFREAFRTAGARSVMSAYNSLDGSPCTANDWLLNRKLKKEWN
                     FKGFVNSDACAVGGANVLHYTAKDYAEATADAIRNGLDVIFQTDYNHYTLFKEAFDKG
                     MIPQPVTDSAVSRVLRAKFELGLFENPYVDMREAEKWNGNPDHRAIALQAARESIVLL
                     KNENDILPLKTGLKKIAVIGSDAGEARLGGYSGPGINKVSILEGIRKLAGDSLIIGYA
                     TGPGRNFQPFERVPSECLVAQTGSAQEHGLKGEYFDNTSLSGTPVMTRVDPQLTFGWT
                     LYPPDPKLAPDCYSVRWAGTLIAPESGLFKLGIEGNDGYRLYLDGKLLIDNWVKCSYS
                     TRLSDVKLEKGKQYEIKVEYFESSGPAKFSLVWNAGVKDTSAEKIKEAVELARSSDVA
                     IVVAGINEGEFNDRAFLKLPGKQEELIHAVNSSGTPVIVVLVGGSAITMSNWIDEAGG
                     IIDVWYPGEAGGDAVADVLFGRYNPAGRLPITFPVTEGQLPLVYNHKPTGRGDDYTDL
                     TGMALFPFGYGLSYTTFVYSDLQFDKPEIEAGETALVTFKVRNTGHWPGDEVCQLYIR
                     DEIASVARPVRELKGFTRIWLSPGETKEVTFRITPELLTMIDIDLKPVIEPGDFRIMI
                     GASSLDIRLRGTLKVK"
     gene            complement(12786..14045)
                     /locus_tag="PKI35_03315"
     CDS             complement(12786..14045)
                     /locus_tag="PKI35_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNX43442.1"
                     /translation="MLTQFLYSTGIYFYGLGIRTGAIFNSKARLWVKGRKRILERISL
                     AMNESRNSGGERKLAWFHCASLGEFEQGRPVMEAFRQQHPEYNIFLTFFSPSGYEVRN
                     AYTGADFIFYLPLDTPNRVSRFIEIVKPDVAFFIKYEFWFNYLSALQQQEIPVFLLSA
                     NFRPNQMFFKWYGEWPRKLLAGFSIIFVQNENSRELLEFINIGNVIVSGDTRFDRVTA
                     IADNAKEFPLIRAFASGHKVVVAGSTWPFDENLLIQYLDADPDVKIIMAPHQTDEAHI
                     LSIMSKANGKAVRLSEARPSSVTMYPMLIIDTIGILPHLYQYGTVAYIGGGFGVGIHN
                     ILEAATFGLPVFFGPNYHKFNEASDLVKLNGAFPIDSYASLADKMAVLLAQPARLSKA
                     SAICKKYVADKKGATEVVLKNIGQFIA"
     gene            complement(14075..14839)
                     /locus_tag="PKI35_03320"
     CDS             complement(14075..14839)
                     /locus_tag="PKI35_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43443.1"
                     /translation="MHHRIFIFLIGFLLVLDNAALSQVRVKDTCISFSMITPTYAMHI
                     PAGDMSSRFGVSNTIGAGFGHKTKTNWYFGIEGNFLFSDKIKNSGSILANISTSDGHI
                     IDENGVYANIVLLERGYDIRLRAGKIIPVGRSNPNSGLMITGGAGFLQHKIRIENSDN
                     AAPQVSGDYKKGYDHMCNGPALNQFIGYIHFSNNRKINCFAGIEAIEAFTRSGRAYYF
                     AEMKKADEKRFDMLLGIKVGWSFPLYKKAGREFYYY"
     gene            15033..16163
                     /locus_tag="PKI35_03325"
     CDS             15033..16163
                     /locus_tag="PKI35_03325"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNX43444.1"
                     /translation="MRSIAMVDLKAQYEKIKPEIDEAIHHVIDTTAFINGPVVKEFQA
                     SLEKYLDVRHVIPCANGTDALQISMMALGLKPGDEVIAPSFTFIATAEVIALLGLTPV
                     LVDVDPDTFNIEPAAIRRAITPKTKAIVPVHIFGQCADMDAVMQIAREHGLFVIEDTA
                     QAIGAEFRFADGTVKKAGTIGHIGCTSFFPSKNLGCYGDGGAIFTNDDNLAGKLRSIV
                     NHGMKTRYYHDDIGVNSRLDSIQAAVLNVKLRHLPEYAIARRKAADAYDAAFKGNPGL
                     KIPTRFAKSSHVFHQYTLVTHGIDRKKLQEYLASKNIPAMIYYPVPIHLQKAYLNPRY
                     KPGDFPVTEALCNAVISLPMHTELDEEQINYITSSVLEFVNA"
     gene            16202..16780
                     /locus_tag="PKI35_03330"
     CDS             16202..16780
                     /locus_tag="PKI35_03330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNX43445.1"
                     /translation="MEKEYFAHPTAIIDEGCVIGKGVKIWHFSHIMSDCVIGDNCNIG
                     QNVVVSPEVILGRNVKVQNNVSIYTGVICEDDVFLGPSMVFTNVINPRSAINRKHQYA
                     KTRVKKGATIGANATIVCGNEIGEYAFIGAGAVVTKPVPDYALVMGNPSRQTGWMSEF
                     GHKLKFDSMGIAVCPESKARYLLRDGKVSRIG"
     gene            16908..17960
                     /gene="rfbB"
                     /locus_tag="PKI35_03335"
     CDS             16908..17960
                     /gene="rfbB"
                     /locus_tag="PKI35_03335"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNX43446.1"
                     /translation="MKKILVTGGAGFIGSHLVRLLVKKYPEYRIVNMDKLTYAGNLAN
                     LSDIENLPNYEFVKGDIVDEDFVMMTFDAYRFDGVIHLAAESHVDRSIASPREFIMTN
                     IVGTVNLLNAARFIWKDNFGGKRFYHISTDEVYGSLHDEGKFLETTPYDPRSPYSASK
                     ASSDHLVRAYHHTFGLPAVISNCSNNYGPFQFPEKLIPLCINNIRHGKPLPVYGAGTN
                     IRDWLYVEDHARAIDLIFHRGVDGETYNIGGNNEWKNIDLVKKLCEIMDRKMGRHPGS
                     SEQLITFVKDRAGHDFRYAIDATKLQSTLGWAPLVKFDEGFEKTVEWYLENPEWLDNV
                     TSGNYLKYYETMYHNR"
     gene            complement(17977..18849)
                     /locus_tag="PKI35_03340"
     CDS             complement(17977..18849)
                     /locus_tag="PKI35_03340"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX43447.1"
                     /translation="MKLITTFLFIILVMLIGIMPFFILYRFSDIARFLLQYVFRYRRK
                     VIRNNLSMAFPEMNKREIFRTTVKIYKNLTDVLMEGIKSFCLTRSQVRRRHVFTNPGL
                     IEPYLAAGKSIITVAAHYNNFEWGTLSPGIFTRFNSVAFYKPLSNKYIDRIIRKSRSK
                     FGTTLASIRETSLTFEKFRNIPTIFLMAADQSPSNHAKAIWVNFMGIGTAFLHGPEKH
                     ARINDYPVFYGHIERTRRGFYTCELSLLADHPLELPEGEITRRYAAKIESIIRNKPEN
                     WLWSHRRWKVKRDL"
     gene            19051..20040
                     /gene="mgrA"
                     /locus_tag="PKI35_03345"
     CDS             19051..20040
                     /gene="mgrA"
                     /locus_tag="PKI35_03345"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HNX43448.1"
                     /translation="MSYLPHPSRYNSMTYRRCGFSGIRLPSISLGLWHNFGHVDEFTN
                     SRQILLKAFDMGITHFDLANNYGPPPGSAEENFGKVLKNDLGPWRDELIISTKAGYLM
                     WPGPYGEWGSRKYLVSSLDQSLKRMNLEYVDIFYHHRPDPDTPLEETMAALDHIVRQG
                     KALYAGISNYPAELALEAFRILRQLGTPCLIHQPSYSLFNRWVEDGLLDVLRQEGVGC
                     IAFSPLAQGLLTNRYLHGIPGDSRAAKPHGFLKEDRVAQMLPKIRKLNEIALKRDQSL
                     ARMSIAWLLKDPAITSVLVGASSISQLTDNLGALDNLEFSTEELAAIDQALKG"
     gene            20301..21512
                     /locus_tag="PKI35_03350"
     CDS             20301..21512
                     /locus_tag="PKI35_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43449.1"
                     /translation="MKRPSIAGKIIMALLLVITAAFSAHSQETEIDTVLVNGIMHDNL
                     VLHREADSLRQYIEMLKLNETSLKSQVDSIQLSINQLNNQLGLKKNQNDSLLMERITL
                     NRTILTLKQDILKCNTEMEGKAQLLRDRDFRMATLEAEIKEMKNNAEIRQVKLEGQLD
                     VHNTKIEAKDKEISYLQKSIDEKDRIIREKTDELTQFYREKDNSLRIIDSLTRTLNQK
                     ELDFIKVSERLKIIEAQYNDMSAKQAAATNKKKKIRFIQGVGLKNYRTPDWQLSLADL
                     SSSSANKIINKNGGNFEFDYMTGVSLSMIDLSKEKGKFTYDAGLFVGFGGANLFKNFY
                     IGPSFKILDYFHVNAGANIAEYTQLQAGFKEGDILTGSTTIPTVKEWKINLYIGFNVD
                     FDLLSSIPKKF"
     gene            complement(21518..22678)
                     /locus_tag="PKI35_03355"
     CDS             complement(21518..22678)
                     /locus_tag="PKI35_03355"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNX43450.1"
                     /translation="MKLYSRLPVLIVLFIPAILLLLPGCHPDDSMPEPTPYTLAVPRY
                     FPTNLNIPSDNPLTVEGIELGRYLFYDGRLSGRTDPDSLMSCGTCHLQSKSFECGIDH
                     PKFTGGRTFGLAGNQTPHYMLPLINLVWNQSGYFWSGMISSASADPNRRTLEDVVRMG
                     VMAKHEMAGDTVRTKTLIQQTPGYPELFEKAFGSKTVTFKNISRAIAQFVRILVSSDS
                     RFDRYMRGEEQLTTGELNGYVLFMTEDGGDCFHCHGGSGNPLFTTNLFYNNGKDTVFN
                     DPSDRYAITGDVQDRGAYKAPTLRNVELTAPYMHDGRFKTLEEVIDFYSENVQWSPYI
                     HPLMHHVNEGGVHLSPSEKQDIIAFIKTLRDDSFLTNPAYSKPETFPDGSKQ"
     gene            22934..24016
                     /locus_tag="PKI35_03360"
     CDS             22934..24016
                     /locus_tag="PKI35_03360"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HNX43451.1"
                     /translation="MQYKPGDKVRFLNEKGGGTVMKILNDFMVSVAIEDGFEIPTLSS
                     NLVLVEQGNNQQAIFTHETEKKQEPKVQVPPVTEETEAASSLSKPSTGQDIAHGIYLC
                     YVPADQQWLVTGNIEIFLVNYTVYDLLYSLFLKNEEGGFSGIDYGVLPKESKVRIETI
                     VHEDLNTWTSGTVQLLLHKDTNEKLLMPVSANFRIKGSKFYREDSFHEFGLLRNKKSI
                     VYTLCELDRVQSTAEFLLAQKEGDMPMPEKAKQFRPESIIDLYQTAPREAEIDLHISA
                     LRDRYDQLSPHEILTIQLTTFERLLDSALACNYAKVVFIHGIGNGSLKEAIIQKIREY
                     SDIEFRNAPFTRYGNGAIELILHQNL"
     gene            24300..24968
                     /locus_tag="PKI35_03365"
     CDS             24300..24968
                     /locus_tag="PKI35_03365"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNX43452.1"
                     /translation="MHSRMYVATMPYILRTFLPSSVTWEIETAEKEIFLTFDDGPIPE
                     VTPLILKTLDEYDAKATFFFVGDNVRKHPEEFRMVAEKGHALGNHTFNHLRGWTTSFP
                     RYHENVNKCHDLVPSPLFRPPHGQITPLMARMLYQNYRVVMWSVLSMDFDKRVSPYKC
                     LQHSIANTHNGSIIVFHDSIKARPRMEYVLPRYLEHFSKLGYKFKALNGQLKYPLPHD
                     QEPA"
     gene            complement(24942..26885)
                     /locus_tag="PKI35_03370"
     CDS             complement(24942..26885)
                     /locus_tag="PKI35_03370"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNX43453.1"
                     /translation="MVTMKKQVFTGFLLTVLLSANVLLKAQPDIRIIKEIAASERKGF
                     PVGRLYATSGIENNYDLTYYRFIWKVDPAVHFISGSVTSYFRLEEGNISQVQFELAEN
                     MTADSALNNSAAAMIQHTGNILTIKLNKESARGEIDSVTIFYHGSPSANGFGSFSTDR
                     HEETPVLWTLSEPFGASDWWPSKNDLTDKIDSIDVYILHPEGNLAASNGVLIDEKRMD
                     DSTFVTHWKHRYPIASYLIAIAVTNYTRFTDVYQGGYGSLNILNYVYPEDSAYLREQA
                     SVVLPVMSLFEELFGQYPFHAEKYGQAEFGWGGGMEHQTMTFLGRGAFGHEIIAHELA
                     HQWFGNTITCGSWHDIWLNEGFATYCAGLTYERMFDGYYWPRWKYQNMSYVTMEPGGS
                     VYCDDTASVGRIFNSRLSYSKGALILHMLRWVVGDEAFFAGMRNYLNDPALRYGFART
                     GDFQRNMEAASGVDLDWYFNDWVYQQGYPSYYLTCTQSPDNHAVVKLEQTTSDSSVSF
                     FRLPVPVKFFGDGRDTTITLEHNYSGEVFSIVPDFRIDSIQIDPERWIISAGNKVDLN
                     QPEGDYLMISPNPAGRTITVFYNPVFSGEICITGMDGRRHNPQFMGNSPGWALLNISN
                     LAEGMYFLSIKGRKGSGRFLVVR"
     gene            26998..28704
                     /locus_tag="PKI35_03375"
     CDS             26998..28704
                     /locus_tag="PKI35_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNX43454.1"
                     /translation="MKKADFHNRLIKISALLFLMTASVSCAIQQAPGGGPKDEKPPQF
                     IGSEPGINTVNFSGKKIMLTFNEYVKLKNIEKFLLISPPLNQQPDIREAGKSLQIKIK
                     DTLRDNTTYKFYLGDAIVDITEGNAVRNFSYAFSTGPVIDSLTLQGKVTGAGDLEPKK
                     DVFVMLYRSTADSAPMLDRPDYVTRTYDNGDFVFTNLAAGKYRIVSIKDLNGDYKYSP
                     GTEEIAFDDSLAEPEYAVIAGQDSVGRIALSGGLKKPLELRLFTEPDSAQRILKSNMI
                     TQNQLQLVFRYPTRNLQLIPLGADNQRRWDFREFSSHNDTVQCWLIPPVSDTLRLQVI
                     DNGNKPDTITLATAVKSKESGRGKNTPQRETLSFVSSVSQSRILELNRPFFITASSPL
                     VSLDTSLILIINAAAHDTAKPQSAVRVDSIGRNIIIRHPWAEGNDYQILLPKGAATDI
                     YNQTNDSLTFAFKVRTKEEYGLIRIRLDGEKLTEPLIFQLLNEKGIVVAEKKDVTGNL
                     LVFDYLLPGKYRMRAIADANRNGKWDTGVYLKHQQPELTFLLPKVLEVRSNWELEESW
                     TF"
     gene            complement(28728..29033)
                     /locus_tag="PKI35_03380"
     CDS             complement(28728..29033)
                     /locus_tag="PKI35_03380"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNX43455.1"
                     /translation="MTEQLKLDVVKLEAAASKLRAMAHPMRIAIIELLNNNSKMNVTE
                     IYETLRIEQAAASHHLNILKSKGILSSKRDGKQIHYSLKNETLLDIISCINRCNEIS"
     gene            complement(29336..30967)
                     /gene="ade"
                     /locus_tag="PKI35_03385"
     CDS             complement(29336..30967)
                     /gene="ade"
                     /locus_tag="PKI35_03385"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNX43456.1"
                     /translation="MEKSFIISGNIVDVVRGVIFKGTVTVENGIIASVTESGCVDGQY
                     ILPGFVDAHVHVESSMLVPTEFARLAVVHGTVASVSDPHEIANVLGLDGVKFMIENSR
                     KTPFRFCFGAPSCVPATPFETSGATLGLAEVEELLKMQEIGYLAEMMNFPGVLSGNAE
                     VLAKLDLAKRFGKPVDGHAPGLKGEDATCYIGAGITTDHECYTLDEATEKIGYGMHVL
                     IREGSAARNFDTLLPLLKHHPGKVMFCSDDKHPDDLVEGHINLLVKRAIKSGYNAMDV
                     IRACTLNPVKHYNLACGLLRPGDSADFIVADNLHDLHIHSAFIRGEKVAENGVSLLPH
                     LVACTPNCFHAGLITPESLQIPATGSTVNVIGACDGDLVTGQLQAQVLNENGLAVTDP
                     SRDLLKIAVINRYTSSAPALGFIMGIGLKNGAIASTVAHDSHNIICAGTNDQDMTRAI
                     NLLIEHKGGIAAVNGGEAEILPLPVAGLMSECDGYEVARKYQQINRMVKSLGSPLHAP
                     FMTLSFMALLVIPSLKMSDKGLFDGNGFRFRELFV"
     gene            31454..33919
                     /locus_tag="PKI35_03390"
     CDS             31454..33919
                     /locus_tag="PKI35_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX43457.1"
                     /translation="MRYLIIGGVAGGASTAARLRRLDEKSEIVLFERGEYISYANCGL
                     PYYIGGAISERDKLFVQTPESFGKRFNVNVRNLSEVLSINRAEKNVTVKDIRTGNTYT
                     ENYDKLVLSPGAEPVKPPLPGINLEGIFTLRSVPDSDKIKHFVEEKKPHHAVVVGAGF
                     IGLEMAENLSHQGINVTIVEMADQVMSPLDYSMAALVHQHLKTRNIEFYLNTAVASFI
                     KENDHIIVKLNSSRTITTDLVVLSIGVKPDSRLAKEAGLQTGNTGGIIVNEFLQTSDN
                     DIYAVGDAIEFTNPVTGTSMITYLAGPANKQGRIAADNLVRGNKKSYRGSVNTAIAKV
                     FDVTVASTGVSGKMLGKLGIPHIDSITHSSSHAGYYPGALPLSIKISFAPDDGRLLGA
                     QIVGFDGVDKRIDLLAEAIRHKATVYDLTQIEHAYAPPYSSAKDPVNIAGMVAENILD
                     GTTKTIWWREVFEDTEREMFLLDVRTRDEYQLGTIEGSVNIPLDELRLHLNELPRNKK
                     MVVFCGVGLRGHVACRILMQSGFSEVYNLAGGMKTYETATQKQSNEDIFSNDYIGHDD
                     LIYQGSKEGKPVVAVNTIDVDACGLQCPGPVLKLKQSIEALAVGEAVRIKASDAGFYK
                     DVKAWANMTGNELVELTQDGALITAVVKKAGTPQKKLAQSGGNNATLICFSDDLDKAL
                     ATFVIANGAASTGKKVTIFFTFWGLNVIKKQKKSPVKKDLFGKMFGIMLPSSSKKLGL
                     SKMNMAGIGGPMMRMVMKDKHVDSLENMIQTALNNGVEFIACQMSMDVMGVKAEELME
                     GVQVGGVATYLERTESANLNLFM"
     gene            34167..34340
                     /locus_tag="PKI35_03395"
     CDS             34167..34340
                     /locus_tag="PKI35_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43458.1"
                     /translation="MRFYSAGYIFLICGLPVAGGNNSLKNKIVHHINDVAADGKWGFN
                     KSKILKMYNRLLW"
     gene            34872..36062
                     /locus_tag="PKI35_03400"
     CDS             34872..36062
                     /locus_tag="PKI35_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNX43459.1"
                     /translation="MRFLSAGYIFPISGPPIAGGILVMDDEGFVAEVLRPGQENLPDE
                     NIEPFDGILCPGFVNAHCHLELSWAKGLIESGIGLDGFLRALDELRHNELPVDPVKAI
                     KEEGAEMHRSGVVAAGDICNSALTVAFKESSGMLFHNFVEVFASDPAKAEPAAMKGIQ
                     LAQIFRALQRNNRASVTPHAPYSVTEQLFRLIGFLPENTPVTIHHQESAAENRFFLNG
                     SGSMAARRLYYNPGLAPFRPTGRRPFESIAPFLNPEQKILLVHNTFSEQEDIGFALQH
                     FPGVAWCFCPNANLFIESRLPDIPLFVKNHCKIMLGTDSLASNRRLSILEEIKTISAH
                     FPSIPLNELLTWATLNGAEFYGFRRLGSFEKGKKPGVVFINNPEPGSMNLTPASTSRL
                     IIPA"
     gene            36065..36265
                     /locus_tag="PKI35_03405"
     CDS             36065..36265
                     /locus_tag="PKI35_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43460.1"
                     /translation="MRIFINRKEITIFAGARVLDAVRAYSVRSAKRLLNGSLSVFDRF
                     GNPTEPDGRLTDGQILSIRKTK"
     gene            36268..37719
                     /locus_tag="PKI35_03410"
     CDS             36268..37719
                     /locus_tag="PKI35_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNX43461.1"
                     /translation="MKKILLPFILSLSFFIVITQPSFCQKNNGPVEVIILHTNDMHAK
                     IDNMAKLAYLADSLRKTHPYVFLFSAGDNFTGNPVVDMVADKGYPMIDLMNRCGFTLS
                     AMGNHEFDLGQEMFNRRQKQACFPFISCNIDASGAILKQPKPYYILKAGKIRIPVLGI
                     VELGENGLPDSHPSRLGGLKFTDGIEKAKEFTWLKDKYGMLIALTHLGVESDEPLAKA
                     MPQFDVIIGGHSHTTMTTPMMVNGVMIVQTGSGLKNVGKVTLTIENRKITSRKYELIP
                     LASIRNGNPTVQAAIDKYNDNEELNRVIAQAENDFNGENALGGLMTDAITSMLSTDMA
                     FQNTGGIRISSIPQGEVKFKDVYRLDPFGNEVIVFKMNLAEIQSLIGNAFDRGKQVDL
                     IPSGVNYTVVTDKQGLCKSVELSDIKGNPLDPGKEYTVGVNSYIAASYKFNHRDAGTS
                     QYKTTAQTLIDYLAKEKNIRYSGVERTTVKAGE"
     gene            38309..39856
                     /locus_tag="PKI35_03415"
     CDS             38309..39856
                     /locus_tag="PKI35_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43462.1"
                     /translation="MNKKIILAGIFLSLFWPAQAQMKNLGEIMDNSNLLLQDILYNSD
                     NSIFGYIFIYSVDKVKEKDWKCEYLILDKNLNEIARNTFIQHKYTSRTIIQFIQCRKK
                     GDNVLVTIGYAYTKVFAAANNYFNYLLTTYRSISLKENVISDEFYYDDGQFKTVNVTD
                     DNLYNMYKKVDNVFEIYPIDNEHYTGYLVQETYKEAKDEYQIKELRVIDINKNLVWAY
                     KFNQSATRNSYSRITKIYWPDETILITFSNFEKDKFTGNSLVSIDLLTGKKLFEYVVE
                     DKNSAYYHNFKTEKYGDKIYLTGTYSPNDERRYIWEDRIGWFKTELDKDGKQVMKKYH
                     PWTDASKFIQIKENGKLEDGYRLNSKEFLIFKDGSVSYLAEKFNEKKGASAFAGYDKS
                     KSSDMVLVNFDDNFEIKSLNVIEKEVSTTFHYDYLFSQYIKNHTGAVFFFEDYKKSDT
                     DKKSIWYLGINKIIDGNYSYEEIPITSPKKEYVIVPYIAKEGYIMLREYNEKDKFNQI
                     RLERLNY"
     gene            39898..41223
                     /locus_tag="PKI35_03420"
     CDS             39898..41223
                     /locus_tag="PKI35_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNX43463.1"
                     /translation="MRYFLLLLALLIICVAPAQHYIPIDTADHQLREIHIKEYKKADA
                     VFISDIRKNYSGREEKQMVEELQDYSDNFIEKIREGQFCFDKRFTEKAEGILSEIRKE
                     NTMVPGNMNILVSKDPSLNAYCLPNGTFIVNMGVFYWLQNTDQIAAVISHEISHIILN
                     HPIRNILNAIRDDLSEESRETLKSVRNQKYNKREKAFELFKRKLYAKGDIKRKHEFEA
                     DSLGYILLRNTDYNCLDFIEALRLNEKYDSLVPDPFPNQVYRDVFDLPNQKFREEWLK
                     MEDFSAYDYSKFKERFDQDSISTHPDTEIRIKKLENLFPELNNSKTNPPDSAFVTLAK
                     IAEYEQVPDLYFAEEYGNGIYLCLERIRQGKDDDFYKEWLGKCFDKIYEARKNYRLNR
                     YLDAINPKTQPLNYQQFLSFMWNLSLEEIKNIRDFYQPSVQNVEQVKTE"
     gene            41537..42817
                     /gene="glyA"
                     /locus_tag="PKI35_03425"
     CDS             41537..42817
                     /gene="glyA"
                     /locus_tag="PKI35_03425"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNX43464.1"
                     /translation="MKKDTAVFNIIRKEKERQMHGIELIASENFVSDQVLKAMGSVMT
                     NKYAEGYPGRRYYGGCQFVDQTEQIAIDRAKTLFGAEWANVQPHSGAQANMAVLMTVL
                     KPGDTFMGLNLSHGGHLSHGSPVNSSGILYNVIAYGVEEETGTINYDKMEEVALEGKP
                     KLIIAGASAYSRDWDFKRMRAIADKIGAILMADIAHPAGLIARGLLNDPIPYCHIVTT
                     TTHKTLRGPRGGMILMGKDFENPWGIKTPKGETKMMSAMLDSSVFPGVQGGPLEHVIA
                     SKAVAFGEALSDEYMEYIIQVKKNARVMSKAFTDKGYKVISGGTDNHLMLIDLRSKFP
                     GITGKLVENTLVKADITINKNMVPFDSRTPFTTSGIRVGTPAITTRGLKEEHMGVIVD
                     LIDEVISDIENEAVIEKVRGKVNDMMSGFPLFAY"
     gene            42972..43466
                     /locus_tag="PKI35_03430"
     CDS             42972..43466
                     /locus_tag="PKI35_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNX43465.1"
                     /translation="MKTVILVRHGKASHDDLSVSDLQRPVTGEGRKRTMKIAKYLSAR
                     ETHVDKIITSHALRATETAKILGKELGITEKDFIIEKAIYGAHPDTFYDILFALPDDV
                     DRVMIVGHNPGIGIFAFRFLKTQVESLPTSGVVALRFITDDWCEVASCRSEVEFTVFP
                     SMIT"
     gene            43534..45600
                     /gene="ppk1"
                     /locus_tag="PKI35_03435"
     CDS             43534..45600
                     /gene="ppk1"
                     /locus_tag="PKI35_03435"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNX43466.1"
                     /translation="MPKKKTLTVNREISWLHFNARVLQEAIDPDTPLLEKIKFLGIYS
                     NNLDEFFRTRVATLNRMSGINKKLYGEVNISPRKTLKEISLIDKQKQIEFQGIFRTIV
                     HELAKNNLFILNEKELTKEQGEFVSAYFQENVRPQLFPIMLGNLKAASLDDRSQYLAV
                     VLQVRNNPEKERYALIEVPVGPLPRFIRLPEEGEKKYLILLDDVIRYCLGDIFPVFGY
                     NLFRAYTIKFTRDAELDIDSDISKSFLEIMSESLKQRDVGSPVRFTYDRHMPEPLLNQ
                     LLSRMQISKRDTVLPGGRYHNFRDFMNFPDPGIPQLTFEKWPPLPHPALSGKESIFSC
                     IRKGDIMVHYPYQSFHHLIDLLREASIDPQVRSIKMTLYRVATSSNVVNALINAARNG
                     KEVTVFLEVQARFDEEANIFWAEKMQEEGVRVIQSIPGLKVHAKLLLIRRHEPGEKNV
                     YYAGISTGNFNETTARVYADSTLFTANKEIASEVNAVFHLFDSKYSVPHFRHLVVAPF
                     SMRSHFIRLINNEIRNARKGKEAWMLLKLNSLADEKIVKKLYEASQSGVKIRLIVRGI
                     CILVPGVSGLSENIEAVSIVDRYLEHTRIFVFCDGGSNKYYISSADWMVRNFDNRIET
                     ACPVYDKSLQQELMSMLQIQLRDNAKSRYLGEKNINEYKEREPGKEKIQSQQELYNYF
                     RNYTLK"
     gene            complement(45624..46073)
                     /locus_tag="PKI35_03440"
     CDS             complement(45624..46073)
                     /locus_tag="PKI35_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43467.1"
                     /translation="MTDTTNQPVPTSAGSMQPGKPINNFLRRLKRVFRWFIFILCLFI
                     AVFIYWKYFFTYSEGYRAGLLQKFSRKGTIFKTYEGEIILSSVQSNKEIMLASEKFFF
                     SVKNEKVVRQLDSIQGHFIVIHYEEKNGVLPWRGESRYLVDSVRVER"
BASE COUNT        13232 a        11018 c        10616 g        11581 t
ORIGIN      
        1 taaactcacc tacttttgca ggagaataca atacttccga gaaatgaaaa tatcatgcaa
       61 ctggctcaat gagtatgtca ggactgacct tgatccggta gaaacagcca gaatcctcac
      121 cgattgcggc cttgaagttg aagcgattga acaatacgaa tcggtgaaag gcggtctgaa
      181 aggggtggtc atcggtcatg tagccgaaac gagacatcac ccgaatgccg ataaacttac
      241 ctgctgcaaa gtggacgtcg gcggggaacg tctccttgat attgtatgtg gcgcccccaa
      301 tgtagccgca ggccagaaag taccggttgc caccctggga acggtaattt ataaaggcga
      361 tgattctttt gtgataaagg ctgcgaaatt gcgcggtgag cccagtgaag gcatgatctg
      421 tgctgaggat gaactgggcc tcggcaattc acatgcagga atcatggtgc ttgatgccgg
      481 tgcacaagtc ggcactccgg cctccgacta ttttggcatt tttacggata ccattctcga
      541 agtagccatc acaccaaacc gcagcgatgc cgcttcgcat ttcggggttg cccgtgacct
      601 ggcagcagcc atcaataaca gggaaaggca gcatccttcg gcatcttttg ttaaaccatt
      661 ggtcgacgca ttcagatccg ataacgacaa tctgcatatt gatgtgtcaa ttgaaaatcc
      721 tgaagcctgt ccccgttatt caggagtcac cctttcggga ttaacggtga aggattcacc
      781 gcagtggctg aaggaccggc tcaaatcggt aggattgcgt cctatcaaca acatcgtgga
      841 tattaccaac tttgtgttga tggaaaccgg gcagccattg cacgcctttg atgctgataa
      901 gatcaacggt aaaaagatcg ttgtaaagaa acagcctgcc ggcaccaaat tcattacact
      961 cgatgaaacc gaaaggtcgc ttggcgccga tgacctgatg atatgcaatg cggctgaagc
     1021 catgtgtata gccggcgttt tcggcgggct gaaatccggc gtcaccgggg aaacgacttc
     1081 cgtattcctc gaaagcgcct atttcgaccc ggtacatatc cggaagacat ccaaactgca
     1141 cggactaaag actgacgctt cgttccgttt cgaacgcggc gccgatccca acataacttt
     1201 gtatgccctg aaacgggcag ccctgcttat aaaagaaatt gccggcggca atatttcgtc
     1261 cgttattgtg gatgtttatc ctgaaccagt cagcaacagg attcttgatg taacgtttga
     1321 cggcatcgaa aaccttatcg gaaagaaaat cgggaaggat gctatccgca acattctgaa
     1381 atcgttggaa atagagatta ttgccgaaac cgacggtgat ttaacgctat cgataccgcc
     1441 attcaaagtg gatgtggaac gcgaagccga tgtggtggaa gaagtcttgc gaatatacgg
     1501 atacaacaat gtggccttca acgaatcggt ccatgcttcg ttatcctata atccgaaacc
     1561 tgatccggaa aaaataatga ataatgttgc cgacctgctt tcggcaaacg ggttccggga
     1621 aatcatgacc aattcgctga ccagcagcgc gtattacgaa aaatcggagg tttatccggc
     1681 tgaccgctgt gtcaggatat tcaatccgct cagccgcgaa ctggaagtga tgcggcaaac
     1741 gctgcttttc ggagggctgg aatctgttgc ccataacctg aaccgccgtc agaacgacct
     1801 gaaattttat gaattcggaa atgtttattc ctacaatcct tccgaatctc acgaggctga
     1861 cgttacagcc agatattccg aaaggaaaaa actttcggtg atgatcaccg gcaaacgact
     1921 gtcggaaagc tggtataaca tttcacagaa agcagattac catcttttga aatcctttgt
     1981 tttccttctt ctccgtaaaa caggagtccc ttactggcaa tggaaaatat ctcctgccga
     2041 tcagaggtta tttacggaag gggaagcctt tgcggttaac ggtaataaca ttttatcgct
     2101 tggaaagctg caaccctcca tcattaagac ctttgatatc aggcaggaag tgttctttgc
     2161 cgagatcgac tgggcacttc tgctcagcct tgtaaaaccg ggcaatagcc ggcaaaagga
     2221 tattccccgt ttcccggaag tgcgtcgcga cctggctttg atcctcgata agggcgtaaa
     2281 gttttccgac attgaagccc tggcctataa gagcgccggc tcaactctga aaagcatcaa
     2341 ccttttcgac gtgtatgaag gggaaaagat aagcgaaggc aaaaaatcat atgccatcag
     2401 tttcacgctg ctcgatgagt cgaaaaccct caccgataaa gaaatagaaa aactaatgga
     2461 caggctggcc aatgacttca ccaaagaact tggcgctatt atcaggaaat agaatttata
     2521 tccaggcatt aaaatctcca aaatatgatg caggcaccca taacctattc agcaagccct
     2581 tccggtgaaa aatttccgct cccgcaaaaa gaggaatatg ctactgagtt tctgaatatc
     2641 agcaaattag ctgaaaatgc ccgtaaagaa ggaaaagaag tcgtggttgt gatgggtgtt
     2701 ggttttgttg gcgccgtgat ggccgctatt attgccgata ccgtcgacag tcagggcaaa
     2761 ccctcaaaat ttgttatcgg ttgccagcgt ccaagtaccc gcagctactg gaaaataccg
     2821 atgctcaacc gcggcgaatc gccggtgaaa gccgaagacc cggaagttga ccgcatcatt
     2881 gcgcgatgcg tcaatcaaaa gcaaacactg atagccacct ttaacagcga ttgcctggaa
     2941 cttgccgacg tagtcgttgt tgatgtgcaa tgcgatttca gcaaacgcga gctcggcaac
     3001 atgaaaaccg gcgaagctga tatggccgcg cttgaagcaa ccctgaagac catcggacaa
     3061 aaaattcagc ctcactgcct caccctgata gaaaccactg ttgctcccgg cactaccgaa
     3121 tttgttgcct ggcctatcct gaaaaaggcc tttgccaaac gaaaaatcac atccgtgccc
     3181 ttactggccc acagcttcga gagagtaatg ccgggacgtg aatatgtatc gagcattcgc
     3241 gatttctggc gtgtatgtgc cggatgcgat aacgaagcac gcaaacgggt agaaaagttc
     3301 cttcgtgaag tgctgaatac caaagacttt ccgctcaccg tcatggaccg tcccattgag
     3361 tcggaaacca cgaaaattgt cgaaaactcc taccgcgcta ccattctggc ctacctgaac
     3421 gaatggagca tcttcgctga acgcaatggc gtagacctcc tgaaggtgat caaagccata
     3481 aaaatgcgcc ccacacacag caatattatt tttcccggcc ccggtatcgg cggctactgc
     3541 ctgcccaagg acggcgggct gggatactgg gcataccggc atatccttgg cttcgatgac
     3601 ggcgatgatg tattcaggat caccccgacg gctatcgata tcaacgatac acgcgccctg
     3661 cacgtagctt cgcttacccg tgatgcatta cgtaatatgg cccgctatat tgccggcgcc
     3721 gatgtgctgc tttgcggcgc cagctaccgc caggatgtgg gcgatacccg ttacagcggc
     3781 agtgaactca ttgtccggaa actcaccgaa atgggcgccg aagtccatat tcacgatccg
     3841 tatgttgaac actggtatga gttggaaagc caggacgaat acccggcccc gggtcattcc
     3901 tggcagcggt ttttccacaa ccaggatcac cttgcggata ccaaagttct gaaagatctc
     3961 cccgaggctc taaatggcat cgaagccctg atacttgccg ttcctcatac tgaatacaaa
     4021 aacctgaaac ccgatgatgt ggtaaaatgg gcaggacagc cgctcgccgt catcgattgt
     4081 tttggcttac tcagcgacga agatatcaga cgctattttg aactcggatg tgaagtaaaa
     4141 gcacttggac gcggtcatat tcagcgcatt aaaaaagaag taaggagagc ggccccaaaa
     4201 aactaagatc aggtaatcct tatcaaatta tattactgaa gattagaaac agcctctcgt
     4261 agaggctgtt tctgatttta ccgcaggaac aacatccgtt tgttcgttaa ttttgccatt
     4321 ttggcagtga ttctgccaaa aaacgttaat catcagcctg tgataataat aatagcgtta
     4381 attttgcatt attactgacg gaaagatcaa gagaagaatg gatattattt caataaagca
     4441 acgatttgga atcatcggca gcgcccccgg gcttgaccgc gccatcgaca ttgccaggca
     4501 ggtggctcct accgatatta ccgtcctgat taccggggag agcggggtgg gcaaggaagt
     4561 atttccccag atcatacacc agcttagcgc ccgtaaacat ggaccttaca ttgccgtgaa
     4621 ctgtggtgct attcctgaag gaactatcga ctcggaactg ttcgggcatg aaaaaggctc
     4681 ttttaccggc gcccacgaag cccgaaaagg atattttgag gtggtgaacg gaggcactat
     4741 ttttcttgat gaagctgccg aacttccgct cgcaacccag gtcaggttac tgagggtcct
     4801 ggaagccgga gaattcatgc gtgtaggatc ttctaaagtt cagaaaacgg atgttcgcgt
     4861 tgtggctgcc accaatgtcg atatacaggc tgccattaat gagggcaaat tcagacagga
     4921 cctgtattac cgcctgaaca ccgttccgat agcaattcct cccttacggg aacgaaaaga
     4981 cgatattttg ttgctgttca ggaaatttgc cgtcgatttt gcagaaaaat accggatgcc
     5041 gccgctcgaa ctcgatgatg aggcaaaaca tatgatgctg aatttcaggt ggcccggcaa
     5101 cgtccgtcag ttgcggaacc ttacggaaca aatcagcatt attgagcatg tgaggacaat
     5161 cacagcacat gtgcttcaga agtatctgcc tgataccgcc aaccgcgaat tacccatttt
     5221 atataccgga tcaaaaccag gcggtgagtt ttcggaacgc gagctgctct ataaggtgct
     5281 gttcgacatg cgtcgcgata ttaatgagtt aaaaaaactt gtcggagata cggccggaca
     5341 cgatcctgtt tccgcccacc tgcattccgg cgttgccggt atgaacgaaa atatgccgtt
     5401 tcccggtaca gaccggcagt ttcatgccgc tcatccttat gtggtggaga atcccgacaa
     5461 tgaggaagaa ctgttttctc aaagcgaggt ggttgatgaa tccttatcaa tacaggaaaa
     5521 agagatagac cttatccgcc gggccctgga aaaacatgca ggccgcaggc gaaatgcggc
     5581 aaaggaactt ggcatttctg aacgcacatt ataccgaaag ataaaggaat acggaattaa
     5641 tgcttaaccg atctctttca agatgaatta taaaacccat tttacttctg cccgctttct
     5701 gatgattaca atggccctgg ctgcgattaa tttattatca ggttgcggaa tatactcatt
     5761 taccggggca tccatttcac cggacgtaaa aacaatttcg gtgaaatact ttcccaataa
     5821 cgcacccctg gtccagccta ccttaagcaa cactttcact gaagcacttc gcgacaaatt
     5881 caccaaccaa accaacctga aattggtaaa aagcggcgga gacctcaaca ttgaaggaga
     5941 aattaccggg tattccaccg aacctgttgc tatccaaggc aaccaacagg ctgctttaca
     6001 gagattgaaa atcaccatca gtgtgcggtt caccaataaa atggacgaaa aacagaattt
     6061 tgaaacatcg ttcagccggt atgaagacta tgatgcttcc tatagcctct cgcaggttga
     6121 aagcgggctg attgaaacta ttacggatgc tcttgcccaa gatgttttca ataaagcagt
     6181 ggtaaactgg taaaaacgga acagaaaaaa atggaacgca ggtcatttga aaaaattctc
     6241 gccgacaggt ctctgcttac cggcagtact gttgagatga tcactgcgct tacccgggaa
     6301 tatccctatt tttccaccgc ctatctttta gtggccagaa accttcggtc gacggataat
     6361 gagtcattta cgccggcgtt gagagtagct gccgcttacg caggtgaccg gaaacgtttg
     6421 aaaatgctca tcgaaagcga tgaaataaag gaattgccct tggccatgca ggacattgat
     6481 ctggcgggga cgatgataga atccggcaca ccggcagccc ccgaaaaaca ggcttcaccg
     6541 gaaacgatct caacggcgcc ggttcaccct gaatcaggtg agggaaaaat accggcaggc
     6601 ggtgttctca tcggcctcat ccgcgacagc ctttccgaaa ttgcagatga aaggaaagag
     6661 aatggtaatg ccgtaagcgc tcatcctcct aaagaaaaag aaacagccgg gaaatccagg
     6721 ttcgaactga tagataagtt catttcggag gaaccccgta tttcaccctc caaacgtgaa
     6781 tttttcagtc ccgaagataa agcgcgccaa agcattgccg accatgatga cctggttagc
     6841 gaaacgctgg cgcacattta tgaaaaacag ggcctttctg agaaggcaat aaagatttat
     6901 gaaaaactaa tattgttatt tcccgaaaaa agtagttact ttgcagcccg cgtggaagaa
     6961 ttaaaaaata atcgtaagta aataaataac aataacttaa caatatctcc tatgaccgct
     7021 tacattctca tttcggtcct tattcttatc gcctgtgtac tgctgacact tgttgttctt
     7081 gttcagaatt caaaaggagg cgggttggca agtaattttt cgtcatccaa tcagttcatg
     7141 ggggtacgta aaactgccga tttcctggaa aaaaccacct ggacgctggc cgttattctt
     7201 ctatcgctca gcctgctttc aatttttgtt atcccccgcc accgcgctaa ccaggctcag
     7261 gctgataccg aacttcgcca gaatattgat gagaatgccc agccggtaca ggattatcag
     7321 cccaacccgg ccaaccaggg caagtaagag tcatcaactg cgatccagat ataaaatgtc
     7381 agaatgtcac ccgattctga cattttttat ttcaggaaaa cagcggatgt attattttta
     7441 cgggtaaatt agatcctgaa attcaaattg atagagatat atccggaaat ggcaggtaat
     7501 cggattttga cagcagaaag cggcatttct gcaatattgt cccgattaaa cctaaatctt
     7561 ccctttggca cgaaatgtga cgaatgttga ccacatttac agaaatctat ccattaatca
     7621 ctaacataaa aatcatttaa ttgtatggca cagttaaaag taaagccttt agcagaccgc
     7681 gtattagttg aaccggccgc tgccgaacaa aaaactgccg gcggaatcat tatccccgat
     7741 actgcaaaag aaaaacccca gcgcggaact gttgtagctg tgggcacagg taaaaaagat
     7801 gaacccttaa ccgtaaaaga aggcgacctg gttctttacg gaaaatattc aggaactgaa
     7861 atcagtattg atggtaagga ctatcttatc atgcgtgaat cggacatcct ggcaataatc
     7921 taagtagtta aaaacaataa tttctgttca catgaaagca gctaaagaaa taatatttga
     7981 tactgaagcc cgcgacaggc ttaaaaaagg tgttgacgcc ctgtcgaacg ccgtaaaagt
     8041 cacccttggc cccaaaggcc gtaacgtgat tatagaaaaa acctatggcg cacctgctgt
     8101 tactaaggat ggcgttacgg ttgcaaagga aattgaactg aaagattccg ttgagaatat
     8161 gggcgcccag atggtgaagg aagtggcttc gaaaacatcg gatgttgccg gcgacggaac
     8221 aacaaccgca acagttttag cccaggccat catcaccact ggtttaaaga atgttaccgc
     8281 cggggctaac ccgatggatc tgaaacgcgg tattgataaa gcggttgcca ctgtcattga
     8341 aagccttaaa aaacaatcaa aagaagttgg cgacagcctc gaaaaaatag aacaggttgc
     8401 caccgtatca gccaataatg actccaccat cggcaaactg atagccgaag ccatggccaa
     8461 agtgaagaaa gaaggcgtca tcaccatcga agaagccaag ggcatcgaaa ctacagtgaa
     8521 agtggttgaa ggtatgcagt ttgatcgcgg ttacatttcg ccttacttcg ttaccgacgc
     8581 tgaaaagatg gaagctgctt acgaaaaccc gtacatcctt attcacgata agaagatcag
     8641 cgggatgaaa gacctgcttc ctatccttga aaaagttgtt caaaccggca ggcccatgct
     8701 tattattgcc gaagacgttg acggtgaagc gctggcaacc ctcgttgtga ataaaatccg
     8761 tggttccctt aagatcgttg ccgttaaagc tcccggcttc ggcgaccgcc gtaaagaaat
     8821 gctgcaggat atcgccatcc tcaccggtgg aaccgttatc agcgaagaac aaggctatcg
     8881 tctggaagac gctgaccttt catatctcgg acaagctgaa aaagtaagca tcgacaaaga
     8941 taacaccacc atcgtcagcg gtaaaggcga acacgatggt atccaggccc gtatcaacca
     9001 gatcaaggct cagatcgaaa ctaccacatc ggactacgac cgtgaaaaac ttcaggaacg
     9061 ccttgcaaaa cttgccggtg gcgtagccgt tattcaggta ggagctgcca gcgaagttga
     9121 aatgaaggaa agaaaagacc gtttcgacga tgcattgcat gcaacccgcg ctgccaatga
     9181 agaaggtatt atccccggtg gcggtgtcgc tttcctgcgt gcactcgaaa ccctcaccac
     9241 cctgaaaact gaaaatgaag atgaagagat aggggtgaac atcgttcgcc gcgcactgga
     9301 agaaccgttg cgccagattg ttgccaacgc aggacttgaa ggttcaatca tcgttcagaa
     9361 ggttaaggaa ggaaaaggcg atttcggatt caacgcccgt accgaaactt atgaaaacct
     9421 tctcaaaacc ggtgttatcg accccaccaa ggttgcccgc gtagcgcttg aaaacgccgc
     9481 ttcaatcgcc ggcatgctgc tcaccaccga gtgcgtattg gccgaaatca aggaagaaaa
     9541 agaacccccg atgccgaatc caggaatggg cggcatgggt ggaatgtact aagatttcac
     9601 tttcccggtt actttttgaa agcttgccgt tgtcggcaag ctttttttgt gatttttcca
     9661 ttcttcctgt tcaattcact gaacttataa cgaacctttg aaatcatgtg caggagactc
     9721 tgctcttcat catattaacc cgcatgtccg ccaagtcata cccggaagct gcagcggcta
     9781 aaatcattgc acaggattca tccgttacaa ccgactttat taatattttt acaaacaaac
     9841 agcatcatac catgaagaat ttttcattcc gtaaatggac catcctgtca atagtcctgc
     9901 tggccatacc atcagtgaca tattcacaga aaaagcttcc gtaccaggac ccggcactta
     9961 gcgccgaagc ccggacaaaa gacctgctcg gcagaatgac accggatgaa aaattctggc
    10021 agttatttat gattcccggc gacctgagtc tgggaaaaca gaatctgaaa agcgggattt
    10081 tcggtcttga ggtggatgcc aggggcgaaa caaaggatgc cgccgggcag atgcttactt
    10141 acggaggaaa agaagacgcc ggcctgtatg ccaataaggt gaatgaaatc cagcgctatt
    10201 ttgtgaatga aacccggctg ggaataccgg tcattccgtt tgctgaatcc ctgcatggcc
    10261 ttggcatggg cggaagcaca tctttcccgc aatcaatagg actggccgca acatgggata
    10321 catcgctgat gcgtaacgta tcccgcgcca ttgccaggga agtgcggtcg aggggcattc
    10381 ggcaggtgct cgacccggtc atcaacattg cccgcgatgt tcgctggggc agggttgaag
    10441 aaacctatgg cgaagatcct ttccttacaa cgatgatggg catctgttat atggatgaat
    10501 ttgaaaagct tggcgtgatc actacgccaa aacattttgc cgtaaacgtc ggcgacgggg
    10561 gccgcgatag ttacccaatc cattacaacg aacggctgct cgaagagata tatttcccgg
    10621 cattcaggga agctttccgg actgcaggag cccgttcggt gatgtcggct tacaattcgc
    10681 tcgatggaag cccttgcacc gccaacgact ggcttttaaa caggaaactg aaaaaggaat
    10741 ggaattttaa agggtttgtg aattctgatg cgtgcgctgt gggcggggcc aacgtgttgc
    10801 attacacggc taaggattat gccgaagcaa ccgccgacgc catcaggaac ggactggatg
    10861 tcattttcca gactgattac aaccactata ccctatttaa ggaagcattt gataaaggta
    10921 tgatccccca acctgtaacc gacagcgccg tttcaagggt gctgagagcc aaattcgaac
    10981 tcggcttgtt cgaaaatcct tatgtcgata tgagagaagc cgaaaaatgg aacggcaatc
    11041 ctgatcatcg tgccatagcc cttcaggctg cccgtgaatc aatcgtactc ctgaaaaatg
    11101 aaaatgatat ccttcccctt aaaacaggcc ttaaaaagat agccgtcatc ggcagcgatg
    11161 ccggagaagc aagattggga ggatacagcg gacctgggat caataaggtt tccatcctgg
    11221 aaggcatcag gaaactggcc ggcgattccc tgatcatcgg gtatgccacc ggtccgggaa
    11281 ggaattttca gccttttgaa cgggtgcctt ccgaatgcct tgttgctcaa accgggtcag
    11341 cccaggaaca cggcctgaaa ggcgagtact ttgataatac ctcccttagc ggaacaccgg
    11401 taatgacaag ggtcgatccc cagcttacct tcgggtggac cctttatccg cctgatccga
    11461 aacttgctcc cgactgttat tccgtgcgtt gggccggcac tttgattgct cctgagagcg
    11521 ggctttttaa actgggcatc gagggaaatg acgggtaccg gctttacctg gacgggaaat
    11581 tgcttatcga taactgggtt aaatgttcct actcaaccag gttgtcagat gttaagctgg
    11641 aaaaagggaa gcaatacgaa ataaaagttg aatacttcga aagttcaggg cctgcaaagt
    11701 tcagcctggt gtggaatgcc ggagttaagg atacctctgc cgaaaaaatt aaagaggctg
    11761 tggaacttgc ccgttcatcg gatgttgcca ttgtggttgc cggcatcaac gaaggggaat
    11821 tcaacgacag ggcatttctc aaattacctg gtaaacagga agaactcatc catgccgtaa
    11881 attcgtcagg tacgcccgtg atcgtcgtcc tggtcggcgg cagcgctatt actatgagca
    11941 actggattga tgaggcaggc ggaatcattg atgtctggta tcccggcgaa gccggcggcg
    12001 atgccgtcgc agatgtcctg ttcgggaggt acaacccggc aggcaggtta ccgataacct
    12061 tccccgttac cgaagggcaa ttgccgcttg tatataacca taagcctacc ggcagaggtg
    12121 atgattacac cgatttgaca ggaatggcgc tcttcccgtt tggttatgga ctgagctata
    12181 ctacttttgt atacagtgat ttacaatttg acaagccgga gattgaagca ggcgaaacgg
    12241 ctcttgttac gttcaaagtc aggaacacag gccactggcc cggggatgag gtctgccagc
    12301 tttatatccg ggatgaaatc gcttctgttg cccgtccggt cagggagctg aaaggattta
    12361 ccaggatatg gctgtccccg ggagaaacaa aagaggtgac tttccggatc acaccggagt
    12421 tacttacgat gattgatata gacctgaaac cggtgataga acccggcgat ttccggatca
    12481 tgatcggggc atcctccctg gatatcaggc tgcgagggac actgaaagta aagtagcatt
    12541 ctgaaatacc gttgatagat gcggcatgaa ttccggccac gaaaccggca aggatcatgt
    12601 ttacaaaagc aaatcctgct ttcaggaata ttgccgtttg taaacatctc agtatctgtc
    12661 acaacggatg caacggcttt aaatttccct gtaaaagttt acattatttc agcaggatga
    12721 atacatcccg caacctttca gatgcacagg aaatgtttct tcccggatca cagataaggg
    12781 agttttcagg ctatgaactg cccgatgttt ttaagcacaa cctctgtggc gccttttttg
    12841 tcggccacat attttttaca tatcgccgat gctttcgaca ggcgggcagg ctgtgccagc
    12901 aacacggcca ttttatctgc caggctcgcg taactgtcga tgggaaaagc cccgttcagt
    12961 tttacaaggt ctgaggcttc attaaattta tgatagttgg ggccgaaaaa taccggcaac
    13021 ccaaaggtcg cagcttccag aatattatgt atgccaacgc cgaaccctcc tccgatgtat
    13081 gccactgtgc catactgata gagatgggga agtattccga tagtatcaat gatcagcatc
    13141 gggtacatcg taacggagga aggacgcgcc tcagacaatc ttactgcttt tccgtttgcc
    13201 ttcgacatga tggatagaat atgggcttca tccgtctgat gtggcgccat gattatttta
    13261 acatcaggat cagcgtcaag atactgaatc aggagatttt cgtcaaacgg ccaggtactc
    13321 ccggcaacaa ccaccttgtg gcccgaggca aaagccctta ttaaggggaa ttcctttgca
    13381 ttatcggcaa tggctgttac acggtcgaag cgcgtatcgc cacttacaat tacattgccg
    13441 atgttgatga attcgagaag ttcccgggaa ttttcattct gaacaaaaat gatggaaaaa
    13501 ccggcaagca gttttcgcgg ccattcgcca taccatttaa agaacatctg gttgggacga
    13561 aaattggcag aaagaaggaa aacggggatc tcctgttgtt gtaaagcgct gagataattg
    13621 aaccaaaact cgtacttgat aaagaaagca acatcgggtt ttacaatttc aataaaacgc
    13681 gatacccgat tgggcgtatc aagcggaagg taaaatataa agtcggcccc ggtataggca
    13741 ttgcgcactt catatcccga cgggctgaaa aatgtcagga aaatattgta ctcaggatgt
    13801 tgttgcctga aagcttccat taccgggcgc ccctgttcaa actcccccag cgacgcacag
    13861 tggaaccagg ccagcttacg ttcgccgccg gaattacggg attcattcat tgcgagcgat
    13921 atccgttcca aaatccgctt acgcccttta acccataatc ttgcttttga attaaatatg
    13981 gctcctgtac gaatgcctaa tccgtaaaaa taaatccctg tgctgtaaag gaattgggtt
    14041 aacattggct gaggctcagt taagtggcta tggtttagta ataatagaat tcacggccgg
    14101 cttttttata caagggaaat gaccagccta ccttaattcc cagcaacata tcgaaccgct
    14161 tttcgtcagc cttcttcatc tcggcaaaat agtatgcccg ccccgatcgg gtaaaggctt
    14221 caatggcttc tattcctgca aaacaattga ttttccggtt attactgaaa tggatatagc
    14281 cgatgaattg attcaaagca gggccgttac acatgtgatc atagcctttt ttataatcgc
    14341 ctgaaacctg gggagctgcg ttatcgctgt tttcaatccg aattttgtgt tgcaggaaac
    14401 cggctcctcc ggttatcatc agtccggagt tgggattcga tcttccaacg gggataatct
    14461 ttccagctct caacctgata tcataacccc tctcaagcag gacaatattg gcataaacgc
    14521 cgttctcatc gatgatatgt ccatctgagg ttgaaatatt cgccaggata cttcccgaat
    14581 tttttatttt atcactgaaa agaaagttgc cttcgatccc gaaataccag ttagttttcg
    14641 tcttatgacc aaatccggcg ccaatggtgt tgcttacgcc aaaacggctg ctcatatcgc
    14701 cggctggtat atgcatagca taggtcgggg taatcatgct gaagctgata caggtatctt
    14761 tcacacggac ctgacttaag gctgcattgt ccagaacgag caggaaaccg atcaggaaaa
    14821 tgaatattct atgatgcatg caagggtagc tctaattggc cgcaaaggta agaaattctt
    14881 tatgtgctag atatttgaac tggaaatgca atgatgtttg ttatttcccg ttttgcagga
    14941 aggactctga ttgttgaatc catttcggga tgcttaaacc atccctgaaa acttgctatc
    15001 tttgcctaac ctaactatta aaaataactc ctatgcgcag cattgcaatg gttgacctaa
    15061 aggctcagta tgaaaagata aaacctgaaa ttgatgaagc cattcatcat gttatcgata
    15121 caacagcctt tatcaatgga cctgttgtca aagaatttca ggcaagtctt gaaaaatatc
    15181 ttgacgtacg gcatgtcatt ccatgcgcaa atggtaccga cgcccttcag atttcaatga
    15241 tggcgctggg cctcaaaccc ggcgatgaag tcattgcccc gtcgttcact tttatcgcta
    15301 ccgccgaagt cattgcactg ctgggcctga cgcctgtgtt ggttgatgtg gaccctgata
    15361 cttttaatat tgaaccggct gccatcagaa gagcaattac cccgaaaaca aaagccatcg
    15421 ttcctgttca tattttcgga cagtgcgccg atatggacgc cgtgatgcag attgcccgcg
    15481 aacacggcct tttcgtgatc gaagatacag cccaggccat tggagctgaa ttccgttttg
    15541 ctgatggaac ggtaaaaaaa gcgggaacaa tcggccatat tggatgtact tcgttcttcc
    15601 catcgaaaaa tctgggttgc tatggcgacg gcggagctat ttttaccaat gatgacaacc
    15661 ttgccgggaa gttgcggtca atcgtaaacc atgggatgaa aacacgatac taccacgacg
    15721 atatcggcgt taacagccgc ctcgacagca tacaggctgc cgttttgaat gtaaaactcc
    15781 gtcatctccc tgagtatgcc atcgcccgcc gtaaagccgc cgacgcttat gatgcggcat
    15841 ttaaagggaa tccaggcctg aaaataccga ccaggttcgc aaaatcctcc catgtattcc
    15901 atcagtatac cctggtcaca cacggcatcg accggaaaaa gctgcaggaa tacctggctt
    15961 ccaaaaacat cccggccatg atttattacc cggttccaat tcatttgcag aaagcatacc
    16021 tcaatccacg ttataagccc ggtgatttcc cggtgaccga agccctttgc aacgcagtga
    16081 tctcactgcc aatgcatacc gaactggatg aagagcagat aaattacatc acatcatcgg
    16141 tgctggagtt tgttaatgcc tgaaatattc atcaatgaaa ttcagataag ataacataaa
    16201 catggaaaaa gaatattttg cccatcctac ggccatcatc gatgaaggtt gcgtcatagg
    16261 gaagggcgtg aaaatatggc acttttcaca tatcatgtcc gattgcgtga tcggcgataa
    16321 ctgcaacatt gggcaaaacg tagtcgtatc accggaagtg atcctgggga ggaatgtaaa
    16381 ggtccagaat aatgtctcga tctataccgg cgtgatctgc gaagacgatg tattcctcgg
    16441 gccgtccatg gtttttacca atgtcatcaa tcctcgcagt gctataaacc gcaagcatca
    16501 atatgcaaaa acccgcgtaa aaaaaggggc aaccattggt gccaacgcaa ccatcgtctg
    16561 cggaaacgaa atcggcgaat atgcctttat cggcgccggg gctgtggtaa caaaaccggt
    16621 tcccgattat gcactcgtga tgggcaatcc atccaggcaa accggatgga tgagcgaatt
    16681 cggccataaa ttgaaattcg attcaatggg tatcgctgtt tgtcccgaaa gtaaagcccg
    16741 ttacctgttg cgcgacggaa aagtctccag gatcggctga aaagtatagt tgccggagca
    16801 cttgaatctc agatgcagat tcacctgatc tgaaaacttg tatttttatt tcagtttcac
    16861 atcatcagcc tgttcatttc taaccaacat taaatcaaat ctataccatg aaaaagatat
    16921 tagttacggg tggcgctgga tttatcggtt cgcacctggt aaggcttctg gtaaaaaaat
    16981 acccggagta ccgcatcgtg aatatggata aacttaccta tgccggcaac ctggcaaatc
    17041 tgagcgatat tgaaaacctt ccgaattacg aatttgttaa aggcgatatc gtggatgaag
    17101 attttgtgat gatgaccttt gatgcatacc gcttcgacgg cgtgatccac cttgccgccg
    17161 aaagccatgt cgaccgttct atcgcaagcc ccagggaatt tattatgaca aacattgtgg
    17221 gaacagttaa cctgctgaat gccgcacgct ttatctggaa agataatttc gggggaaagc
    17281 gcttttacca tatcagcacc gatgaggtgt acggttctct tcatgacgaa ggaaagtttc
    17341 tcgaaacaac cccatacgac ccccgaagcc cctattcggc atcaaaagcc agcagcgacc
    17401 acctcgtcag ggcctaccat catactttcg gactgccggc cgttatcagc aattgctcca
    17461 acaactatgg cccgttccag ttccctgaaa aactgatccc gctatgcatc aacaacatcc
    17521 gtcacggaaa acccctgccg gtttatggcg ccggtaccaa tatccgtgac tggttatatg
    17581 ttgaagacca cgcaagggct atcgacctga ttttccaccg gggtgtggat ggtgaaacct
    17641 acaacatcgg cggcaacaac gaatggaaga atatcgatct cgtaaagaaa ctctgtgaaa
    17701 tcatggaccg caaaatggga cgtcacccgg gatcctccga acaactgatt actttcgtaa
    17761 aagacagagc cggccacgat ttccgttatg ccattgatgc aaccaaactg caaagcacat
    17821 taggatgggc tccccttgta aagttcgacg aaggttttga aaaaacggtt gaatggtacc
    17881 tcgaaaaccc ggaatggctc gataatgtaa cttcaggcaa ttacctgaaa tactatgaga
    17941 ccatgtatca caaccggtaa aaaaggaacc cggccattat aaatcgcgct ttactttcca
    18001 ccttcggtgc gaccataacc agttttcagg tttattcctg ataatcgatt caatttttgc
    18061 cgcatacctg cgggtgatct ctccttcggg aagttcaagc ggatgatcgg ccagaagcga
    18121 caattcacag gtataaaatc cccgccgggt tcgttctata tgtccgtaaa acacaggata
    18181 atcattaatc cgggcatgtt tttcaggccc gtggagaaaa gccgtaccga tccccataaa
    18241 atttacccat atcgctttgg cgtggttcga tgggctttga tcggcagcca tcaggaaaat
    18301 agtggggata ttcctgaact tttcaaaggt aagcgaggtt tccctgatcg aagccagcgt
    18361 tgtaccgaat tttgaccggc ttttacggat gatcctgtca atgtacttat tggaaagtgg
    18421 tttataaaat gccacagaat tgaaacgggt aaatattccg ggcgacaggg ttccccattc
    18481 aaaattgtta taatgtgcag cgacggtgat aatgcttttt ccggctgcaa ggtaaggttc
    18541 aatgaggccg ggattggtaa agacatggcg tctccttacc tgcgaacgag tcaggcagaa
    18601 agacttgatc ccctccatca gaacatcggt caggttttta taaattttca cggtcgtcct
    18661 gaatatttcc cttttattca tttcgggaaa cgccatggaa aggttattcc ggatcacctt
    18721 ccggcgatag cgaaaaacat attgcaataa aaaccttgcg atatctgaaa aacggtataa
    18781 gataaaaaac ggcatgatgc cgatcagcat caccaaaatg atgaaaagaa aagttgtgat
    18841 cagcttcatg ctaaatcatc tggcgagaaa gcaaatgtac ttttttcctg aaatcagtca
    18901 aaaccggaaa ttattttcca ctgaatccat tttctgttcg atatcattta acaggtctca
    18961 tttctgaata attcttcgta attttcagat taaatggatg aacagagtga gaaaatcaaa
    19021 ccgattgtca atgaattaaa acccttgtat atgtcctatt tgccccatcc ttcacgttac
    19081 aattcgatga cataccgccg ctgtggtttc agcggtatca ggctgccgtc catctcgctg
    19141 ggcctgtggc ataatttcgg tcatgtcgac gaatttacca acagccggca aatcttgctg
    19201 aaagccttcg atatggggat cacccatttt gatctggcca acaactatgg cccgccaccg
    19261 ggatcggcgg aggaaaattt cggaaaggtg ctgaaaaacg acctcgggcc ctggcgcgac
    19321 gaattgatca tctccacgaa agccggttac ctgatgtggc ccggaccata cggtgaatgg
    19381 ggatccagaa aatacctggt ctccagcctc gatcaaagcc tgaaacgcat gaaccttgaa
    19441 tacgtggaca ttttttatca tcatcgtccc gaccccgata cgcccctcga agagaccatg
    19501 gcagccctcg accatattgt aagacagggg aaagcactgt atgccggcat ttctaattat
    19561 ccggccgaat tggctttgga ggctttccgt atccttcgcc aattgggaac gccctgcctt
    19621 atccaccagc caagttacag cctgtttaac cgttgggtcg aagacggcct gctcgatgta
    19681 ctccggcagg aaggtgtcgg ttgcattgcg ttttccccgc tggcgcaggg tttgctgaca
    19741 aaccgttacc tgcatggcat tcccggggat tccagggccg ccaagccaca cggttttctg
    19801 aaggaagacc gtgtggcgca gatgcttccg aagatcagga aactgaacga aatagccctg
    19861 aaacgcgacc aaagcctggc caggatgtcc atagcctggc tcctgaaaga tccggcgata
    19921 acctcggtgc tggtaggagc cagttcgata agtcagctta ccgataacct cggcgcgctg
    19981 gacaacctgg aattttctac cgaagaactc gccgccatcg atcaggcatt aaaagggtaa
    20041 tcaaaccgga aaggaaaaac ctgtgagcgt ccgccctgcg gacctgacag cgttttcagg
    20101 cgccggaaaa tgctggcaac ctttgccgga tatcatcctg cctgtcagaa agcctgtaaa
    20161 aatccccgac aggcccgaaa tccttccggt taacttgtgc aggccaactg cttaactaat
    20221 ttttaccgac ctttgcacaa taaaacgaca cttagataat tatttcataa agtcgttttc
    20281 aatccatgga aattttattg atgaaaagac cttcaattgc aggaaaaata ataatggcgc
    20341 tattgctcgt catcactgcg gccttcagcg cgcattccca ggaaacggaa attgacacgg
    20401 tgctggtcaa tggcattatg cacgacaacc tcgtactgca tcgcgaagcg gacagtctgc
    20461 gccagtacat cgaaatgctt aaactgaatg aaacctcgct taaaagccag gttgattcca
    20521 ttcaattgtc gatcaatcag ctcaataatc agcttggcct taagaaaaac cagaacgaca
    20581 gcctgctgat ggagcgcatt acactgaacc gcaccatcct caccctgaaa caggatatcc
    20641 tgaagtgcaa cacggaaatg gaggggaaag cccaattgtt gcgtgaccgc gatttcagga
    20701 tggcaaccct ggaagccgag attaaagaaa tgaaaaacaa cgccgaaatc agacaggtga
    20761 aacttgaagg ccagttggat gtccacaata caaaaataga ggcaaaagac aaggaaatca
    20821 gttatctgca aaaatccatc gatgaaaagg accggattat tcgcgagaaa acggatgaac
    20881 ttacgcaatt ctatcgtgaa aaagataact cactcaggat catcgactct ttaacaagga
    20941 cgttgaacca gaaagagctg gattttatca aggtttcgga acgactgaaa atcatcgagg
    21001 cacagtataa cgatatgtca gctaaacaag ccgctgcaac caataaaaaa aagaagatac
    21061 gtttcataca gggcgtaggg cttaaaaact accggacacc cgactggcaa ctctctcttg
    21121 ccgatctgtc cagttcaagc gccaataaaa ttatcaacaa aaacggggga aattttgaat
    21181 tcgattatat gacaggcgtt agcctctcca tgattgacct gagtaaggag aaaggaaaat
    21241 tcacctatga tgccggacta ttcgtcggtt tcgggggagc taacctcttt aaaaatttct
    21301 acatcgggcc gagctttaaa atccttgatt attttcacgt caacgcaggc gctaatattg
    21361 ccgaatacac ccaactccag gcaggattca aagaaggcga tatactcacc ggcagcacga
    21421 ctattccaac agtcaaggaa tggaaaataa acctgtatat cggttttaat gtcgatttcg
    21481 atctgctctc cagtatcccc aagaaattct gaggatatta ctgtttcgat ccgtcaggga
    21541 atgtttccgg tttgctgtaa gccggattgg tcaggaaaga atcgtcccgc aaggttttta
    21601 taaaagcgat aatgtcctgc ttttcggaag gggacaggtg aaccccgcct tcgtttacgt
    21661 gatgcatcag cggatggata tacggcgacc actgcacgtt ttccgagtaa aaatcaatga
    21721 cttcttccag cgttttaaag cgcccgtcat gcatatatgg cgccgtcaat tcaacatttc
    21781 tgagggttgg cgccttgtac gcgcctctgt cctgaacatc gccggttatt gcataccgat
    21841 ccgacggatc attgaaaaca gtgtccttgc cattattata aaagagattg gtagtgaaca
    21901 gggggttgcc tgagccgcca tgacaatgaa aacaatcgcc cccgtcctcc gtcataaaaa
    21961 gcacatagcc gttcagctcg ccggttgtca gttgttcttc gccgcgcata taccggtcga
    22021 atctcgaatc cgacgatacc agaatcctga cgaattgagc aatcgcacgg cttatattct
    22081 taaaagtcac cgtttttgat ccgaaagctt tttcaaagag ctccggataa ccaggggttt
    22141 gctggatgag ggttttcgtc ctgaccgtat cgccggccat ttcatgtttt gccatgacgc
    22201 ccattctcac tacatcttcg agggttctcc tgtttgggtc ggctgaagcg ctgctgatca
    22261 ttcccgacca gaaatacccg ctctggttcc acacgaggtt aatcagaggc aacatataat
    22321 gaggcgtttg attgcccgcc agtccgaaag tcctccctcc cgtgaatttc ggatgatcga
    22381 tgccgcattc aaacgacttg ctttgaagat gacaggttcc gcacgacatg aggctgtcgg
    22441 gatcggtcct gcccgacagg cggccgtcat aaaacaggta acgccccagt tcaatccctt
    22501 caacggtcaa cgggttatcg gaaggaatat tcagatttgt cgggaaatac ctgggaacag
    22561 cgagcgtata tggtgtgggc tcaggcatag aatcgtcggg atgacagcct ggcaacagca
    22621 ataatatcgc cgggatgaaa agcacaatta aaaccggaag cctcgaatac agtttcatga
    22681 aatgactatt tcctgacaca gggcttcatc acagcgcctt ttgccgcata aaagcctgac
    22741 attacaaaaa tacttgattt taacatgccg attaacagcc tggagattcc ccgtccggga
    22801 atcaactttc atccgtgccc gattaatgag tatttttgcg gtactgtaaa ctgttgcctg
    22861 ctcaattata gcggtaacag gagaagtctg atcgatctga tccttatcat taaaaatcta
    22921 aacgttctga tccatgcaat ataaacccgg cgacaaagtc aggttcctga acgaaaaagg
    22981 cggcggcacc gttatgaaaa tcctgaacga cttcatggtt tccgtggcca tcgaagacgg
    23041 tttcgaaatt cccaccctca gcagcaacct cgtccttgtt gaacaaggca ataaccagca
    23101 ggccattttt acacacgaga cagaaaaaaa gcaggaacca aaggttcagg ttccccctgt
    23161 aactgaagaa actgaagcag cctcgtcgct ttcaaagcct tctaccggtc aggacattgc
    23221 gcacggcatt tacctttgct atgtgcccgc cgaccagcag tggctggtaa ccggaaatat
    23281 cgaaattttt ctggtcaatt ataccgttta cgatctgttg tacagcctct ttctgaaaaa
    23341 tgaagaaggc ggattttcgg gaattgatta cggagttttg ccaaaagaat caaaagttcg
    23401 catcgaaacc attgtacatg aggatcttaa cacatggacg tcgggaacgg tgcaactgct
    23461 cctgcataaa gatacaaacg aaaaactgct gatgccggta agcgctaatt tcaggatcaa
    23521 aggctcgaaa ttttacaggg aagacagttt ccatgaattt ggcctgctca ggaacaagaa
    23581 atccatcgtt tacaccttgt gtgaactgga cagggttcag tcgacggcag aatttctcct
    23641 ggcccagaaa gaaggtgata tgcccatgcc tgaaaaggca aagcaattca ggcccgaatc
    23701 cattatcgac ctttaccaga cagcgccccg cgaagctgaa attgatctcc acatatcagc
    23761 gcttcgggac cggtacgacc agttatctcc gcacgaaata ctcaccatcc agcttacaac
    23821 ttttgaaagg ttgctcgaca gcgcccttgc ctgcaattat gcaaaagttg tctttattca
    23881 cggtatcggc aacggatccc ttaaagaagc tattattcag aaaatcaggg aatacagcga
    23941 cattgaattc agaaatgccc cctttacccg ttacggcaac ggcgccatcg aactgattct
    24001 ccaccaaaac ctgtaatccc ggcaaaaagc ctgccgggca aaagcaactg cctgctgttg
    24061 agaatatcgc gtaaaatgag aggaaggcgt atttgccggt tggaaatttt cagaaaaaac
    24121 ggacaaaaca tcccttcaag cccggctttt taaaagcctt ccgcccggat gaagggttcc
    24181 tgattatcgt gattactctt gccttttgct gattgtcaat agtttttaga tttatacggc
    24241 aaaaacgttt accttcgcgc cgcaattttc aacctgaatt attcccaaat ttgctcataa
    24301 tgcacagtcg gatgtacgtg gcaaccatgc cttatatact tcgcaccttc cttccttcgt
    24361 cagttacctg ggaaattgaa acagcagaaa aagaaatctt cctcactttc gacgatggtc
    24421 cgattcccga agtcaccccg ctgatactga aaaccctgga cgaatatgat gcgaaagcca
    24481 catttttctt tgtaggggat aatgtaagaa agcatcccga agaattcagg atggtagctg
    24541 aaaaaggtca tgcactgggc aatcacacct tcaatcacct gcgcggatgg acaacttcct
    24601 tcccccggta tcatgaaaat gtaaataaat gccacgatct ggtaccgagt ccgctattca
    24661 ggccgccaca cgggcaaatc acgccactga tggcccgaat gctttaccag aattaccggg
    24721 ttgttatgtg gagcgtgctg agtatggatt tcgacaagag ggtgtcgccg tacaaatgcc
    24781 ttcagcattc tatcgccaac acacacaatg gttccatcat cgtttttcac gacagcatta
    24841 aggctcgccc ccggatggaa tacgtccttc cccgttacct tgaacatttt tcgaaactgg
    24901 gatataaatt taaggccctg aacggtcagc taaaatatcc gttacctcac gaccaggaac
    24961 ctgcctgatc cttttctgcc ctttattgaa aggaagtaca ttccttccgc caggttactg
    25021 atattcaaca gcgcccagcc tggactatta cccatgaact gaggattgtg tcgcctgccg
    25081 tccatgccgg taatgcatat ttcgccggaa aatacgggat tgtagaaaac cgtgatagtc
    25141 cggccggccg gattcgggct gatcatcagg taatcgcctt ccggctggtt caggtctact
    25201 ttgttgcctg cagaaataat ccaccgttcc gggtcgattt gtatagagtc gatccggaaa
    25261 tcaggcacaa tcgaaaagac ttctcccgaa taattatgtt caagcgtgat ggttgtatcg
    25321 cgcccatccc caaaaaactt aaccggaaca ggcaacctga aaaaagatac cgatgaatcg
    25381 gaagtggttt gctccagttt aacaacggcg tggttatccg gactttgggt gcaggtgaga
    25441 taatatgacg gatagccttg ttgataaacc caatcgttaa aataccaatc aaggtcaacg
    25501 cctgaggcag cttccatatt gcgctgaaaa tcaccggtgc gcgcaaaacc ataccttaga
    25561 gcaggatcat ttaaataatt cctcatcccg gcaaagaaag cttcatctcc gacaacccac
    25621 cggagcatgt gtaaaatcag ggcgcctttg ctgtatgaaa gccgggaatt gaagattcgg
    25681 cctacgctgg cagtatcgtc gcaatacacc gatcctcccg gttccatggt gacatacgac
    25741 atgttctggt atttccacct gggccaataa tagccatcga acatacgctc ataagtaagg
    25801 ccggcacaat aggtggcaaa accttcattc agccagatat catgccatga accgcaggta
    25861 atcgtattcc cgaaccactg gtgggctagt tcatgggcta taatctcatg gccgaaagcc
    25921 cctcttccca aaaatgtcat ggtctggtgt tccatgccgc cgccccagcc gaattccgcc
    25981 tggccatatt tttcggcatg aaacgggtat tgcccgaata gctcttcgaa aagactcatt
    26041 accggcaaca ccactgatgc ctgctcacgg aggtaggccg aatcctcggg ataaacatag
    26101 ttgagtatgt ttaacgatcc gtaaccaccc tggtatacat cggtaaaccg tgtataattg
    26161 gtaacagcaa tggcaatcag gtacgaagca atcgggtaac gatgcttcca gtgagtgaca
    26221 aaggttgaat catccatccg cttttcatcg atgagcaccc cgtttgaagc ggccagattc
    26281 ccttccgggt gaaggatgta aacatcgata gaatcgattt tatcggtcag gtcatttttc
    26341 gaaggccacc agtccgatgc gccgaaaggt tccgagagcg tccaaagcac cggcgtttct
    26401 tcatgccggt ctgtggaaaa cgatccgaat ccgttggcag acggggaccc atgataaaaa
    26461 atggtcacgg aatcgatctc acctctggcc gattctttat tcagtttgat ggtaaggata
    26521 tttccggtat gctgtatcat cgctgccgct gaattgttca aggcagaatc ggctgtcatg
    26581 ttttcggcca gttcaaactg cacctggctt atgtttccct cttctaacct gaaatacgac
    26641 gttaccgaac ccgaaataaa atgtacggca ggatctactt tccaaatgaa acgataatac
    26701 gtcaggtcgt aattgttttc aattcctgaa gttgcgtaaa gacggccaac cgggaaccct
    26761 tttctttcgg atgcggcaat ctccttaata attctgatat ccggctgtgc ctttaacagg
    26821 acattggctg agagtaaaac agttaaaagg aatcctgtaa aaacctgctt tttcatcgtt
    26881 accatttcct gaagtaaagg tacaatttta gcgaagctcc taaacaaatg acaacaggcc
    26941 tctgaggatt accagcgaag cagaattttc acaatctttg catcctcaat catgattgtg
    27001 aaaaaagccg acttccataa cagattaatc aaaatatccg ccctgctttt cctgatgacg
    27061 gcatcggttt catgcgccat acagcaggct ccgggcggcg ggccgaaaga cgaaaagccc
    27121 cctcaattca ttggctccga acccggcatt aacacagtaa atttctcggg taaaaaaatc
    27181 atgcttactt tcaatgagta tgttaaactg aaaaacattg aaaaattcct gctgatttcg
    27241 ccccccctca accagcaacc ggacatcagg gaagccggca aatccttaca aatcaagatt
    27301 aaggatacct tacgcgacaa tacaacctat aaattttacc tgggtgatgc cattgtcgat
    27361 attaccgaag gtaacgctgt ccggaacttt tcttacgcat tttcaaccgg tcccgtaatt
    27421 gactccctga cccttcaggg aaaagtaacc ggcgctggcg accttgagcc caaaaaagat
    27481 gtttttgtca tgctttaccg cagcaccgcc gattcggcgc ctatgcttga tcgcccggac
    27541 tatgtcacca ggacttatga caacggggac tttgtcttta caaacctggc tgcaggaaaa
    27601 tacaggattg tgtcaattaa agatttgaat ggcgattaca agtattcgcc gggaaccgag
    27661 gaaatagctt ttgatgattc gctggcagag cctgaatatg ctgtaatcgc cgggcaggac
    27721 agcgttggga gaattgccct atccggcggg ttaaaaaaac cgctggaact caggctattc
    27781 accgaacccg attctgcaca acggatcctg aaaagcaata tgatcactca aaaccagttg
    27841 cagcttgttt tccgctatcc gacccgtaac ctgcagttga ttcccctggg agccgacaac
    27901 caaagaagat gggatttcag ggaattctcc tcccataatg atacggtaca atgctggctc
    27961 ataccgccgg tttccgatac gctccggctt caggtcattg acaatggtaa caagcctgat
    28021 accataacac tggccactgc cgtaaaaagc aaagaatcag ggcgcgggaa gaacaccccg
    28081 caaagggaaa ccctttcgtt tgtttcatcc gtttctcagt cgcggatact cgaattaaac
    28141 cgcccgtttt ttatcacggc ctcatcccct ttggtcagcc tcgatacttc gctcatcctg
    28201 atcataaacg ccgctgcaca cgacactgct aaaccgcaat cagccgtcag ggtcgacagc
    28261 atcgggcgca acataataat ccggcatcca tgggctgaag gcaacgatta ccagatttta
    28321 ttgccaaaag gcgccgctac cgatatctac aaccagacca acgattccct gacttttgca
    28381 tttaaagtaa ggacgaagga agaatacggt ctcatccgga tccgcctgga tggggaaaaa
    28441 ctcacggaac ctttgatatt ccagctttta aatgaaaaag gaattgttgt ggctgagaaa
    28501 aaagatgtca ccggcaatct gctcgttttc gattacctgc ttcccgggaa atacaggatg
    28561 agagccattg ctgatgcaaa caggaacggg aaatgggata caggagtgta tctgaaacac
    28621 caacaacccg aattgacctt cttacttccc aaagtactgg aagtacgcag caattgggaa
    28681 ctggaagaat cctggacttt ctgaaaatac ctgccggaaa acgatgatta acttatctcg
    28741 ttgcaccggt tgatgcatga aatgatatca agcagcgttt cgttcttcag tgaatagtgg
    28801 atttgtttcc catcacgttt cgatgaaaga atgcctttgc tttttaaaat attcaggtga
    28861 tgcgatgccg cagcctgttc aatcctcaat gtttcgtaaa tctcggttac gttcattttg
    28921 ctgttattat tcagaagctc aataatggca atacgcatag gatgagccat tgcacgaagt
    28981 ttactggcag ctgcttcgag ttttacaacg tctagtttta attgttccgt cattggatat
    29041 tagatttgct acaaaaataa tgaaaaatac attatagcaa aataaatatg taaattttaa
    29101 aatcttttta cagtttactg taaatgaata ttttataaag gtgcctgaat tattcccccg
    29161 ttctacggtt aaaaaaggca ggcagaagat caggcagttt taccattcag tacaagtcag
    29221 aaaatagctg atctcatttt gatgatcggt gtaatggtaa acctgagcat caataatggt
    29281 cagccctgca acctccccca tttaactata tttcagcttt ttcccatttt ccggctcaca
    29341 caaataactc cctgaaccgg aacccgtttc cgtcaaaaag ccctttatcg ctcattttta
    29401 gcgatggaat caccagcaac gccatgaatg acaatgtcat gaacggtgca tgcagcgggc
    29461 ttcccagcga tttcaccatc cggttgatct gttggtattt ccgggccact tcgtaaccat
    29521 cgcattccga catcaaccct gcaaccggca atggcagtat ttccgcttcc ccgccattca
    29581 ctgcagctat gccgcctttg tgttcaatca gcaggttaat tgcccgggtc atatcctggt
    29641 cgttggttcc tgcacagata atattgtgac tgtcgtgcgc cacagttgaa gcaattgctc
    29701 catttttcag accgatcccc atgataaatc ccaatgcagg cgctgatgaa gtataacggt
    29761 ttattacggc tatcttcaac aggtcgcgcg atggatcggt aacggccaga ccattttcat
    29821 tcaaaacttg cgcctggagc tgaccggtga ccaagtcacc gtcgcaggct ccgattacgt
    29881 taaccgtgct gcctgttgcc ggaatctgca gactttcggg cgttatcaat ccggcatgaa
    29941 agcaattagg tgtgcaggca accagatggg gcaataatga aacaccgttt tctgcgactt
    30001 tttcaccgcg aataaatgcc gagtggatgt gcaggtcgtg caggttatcg gcaacaatga
    30061 aatcggcgct gtcgccgggt cgcaataatc cgcaggcaag gttgtaatgt tttaccgggt
    30121 tcagcgtgca ggcccggata acatccatgg cattgtaacc gctcttgatg gcccttttca
    30181 ccagcaggtt gatgtgccct tccacaagat catcaggatg tttatcgtcg gagcagaaca
    30241 ttacctttcc gggatgatgc ttcaacaacg gcagtaacgt gtcgaagttg cgggcagcgc
    30301 ttccttcacg gatcaggacg tgcattccgt aaccgatctt ttccgttgct tcgtccagcg
    30361 tatagcactc atgatcggta gttataccgg ccccgatata acatgtggca tcttcacctt
    30421 ttaaaccggg ggcatggccg tcgaccggct tgccgaaacg cttcgccagg tcaagcttgg
    30481 ccaatacttc ggcattgccg gacaaaacgc cgggaaaatt catcatttca gccaggtaac
    30541 cgatttcctg cattttgagc agttcttcaa cctcggccag gccaagagtg gcgcccgatg
    30601 tttcaaaagg cgttgccggc acacaggaag gcgccccgaa acagaagcgg aatggagttt
    30661 tacggctatt ctcaatcatg aatttcacac catcaagacc aagaacattc gcaatttcat
    30721 gcggatcgga aaccgaagca acggtgccgt gaactactgc cagccgggca aattcagtgg
    30781 gcaccagcat ggaactttca acatgaacat gggcatccac gaagccgggt aggatgtact
    30841 ggccgtcaac acagccggat tcagtaaccg aggcaatgat cccgttttcg acagtaacag
    30901 ttcctttaaa aataacaccg cgtaccacat ccacgatgtt gccgctaatt atgaatgatt
    30961 tttccataaa gacaaaagta ggaaaatacc cgactggctg cgacgaacct caagccccgg
    31021 atttcatgct tcctcacgcc gtctttgcct ccggaaggcc atccgtgttt caagccattt
    31081 ttcagagaat caaattattg aaaaagtaaa gcctccgtca atttaacagg cgacttgctg
    31141 ttcccggccc gattcaataa atgcggatga acaggataaa taattgttga aatctctgta
    31201 agcggcatta ccgcacgtgg cctggcgcta tgctaacctt atgtcaaata aggtcatgct
    31261 gatttttctt cagcctgaat atatggatat tttccgaata cttaacatca gataaccaat
    31321 tgatattcaa ttattcgtaa gggcgcaaca aaaataattt ataaccagac aacaaaatgg
    31381 ctgatccgtt gttaacaata actgatatct tgcgacatat ttaaaaaaca gcataattaa
    31441 tacatcagca aacatgagat accttattat cggaggagta gcaggcggag cttcaacggc
    31501 ggcccgttta agaagattgg atgagaaaag cgagattgtt ctctttgaaa gaggcgagta
    31561 catttcgtac gcaaattgtg gtttgcctta ttacatagga ggcgctatca gcgaaaggga
    31621 taaacttttc gttcaaacgc cggaaagttt cggcaaaagg tttaatgtca acgtgcgaaa
    31681 cctgagtgaa gtattatcca taaaccgggc agaaaagaat gtaacggtca aagatatccg
    31741 taccggaaat acctataccg aaaattacga caaactggtc ctttcgcctg gcgccgagcc
    31801 ggtaaagccc ccgttgccgg ggataaacct tgaaggcatt tttaccctga ggagcgttcc
    31861 cgattccgat aaaatcaaac attttgttga agaaaagaaa ccgcaccatg ccgttgtggt
    31921 aggtgccggt tttatcgggc tcgaaatggc cgaaaacctc agtcatcagg gaataaacgt
    31981 taccattgtt gagatggctg atcaggtaat gtcccccctc gattattcca tggctgcatt
    32041 agtccaccag catttgaaaa ccaggaatat agagttctac ctgaatacgg cagtcgcttc
    32101 ttttattaag gaaaacgatc atattattgt caaacttaat tcttcccgga cgattacaac
    32161 tgacctggta gtattgtcaa tcggcgttaa acccgacagc cgcctggcaa aagaggccgg
    32221 attgcaaacc ggtaatacgg gcggcattat tgtcaacgaa tttctgcaaa cttccgacaa
    32281 cgatatatac gccgtaggcg atgccattga atttaccaat cccgttaccg gtacgagcat
    32341 gataacctat ctggccggtc ctgccaataa acagggtcga atcgcagcag acaatctcgt
    32401 caggggcaac aaaaaaagtt ataggggctc tgtaaatacg gctattgcaa aggtttttga
    32461 tgttaccgtt gcttcaaccg gggtttcggg caaaatgctt ggtaaactgg ggattcccca
    32521 tattgactca atcacccatt cctcgtcaca tgcagggtat tatccgggag ctttgcccct
    32581 gtcgataaaa atatcctttg ctcccgatga cggacggttg ctgggggctc aaattgttgg
    32641 tttcgatggc gttgataaac gtattgatct tttggccgaa gccatacggc acaaggctac
    32701 ggtctatgac ctcacccaga ttgaacatgc ttatgccccg ccgtactcct cggctaagga
    32761 tcctgttaat attgccggaa tggtagctga aaacatcctg gatggaacga ctaaaacgat
    32821 ctggtggcgt gaggtttttg aagataccga aagagagatg tttttactcg atgtgcgtac
    32881 ccgcgacgag taccagcttg gaactatcga aggttccgtc aatattcccc tggatgagct
    32941 gcgccttcac ctgaatgaac ttccgcgcaa taaaaagatg gtagtttttt gcggagtcgg
    33001 gcttaggggg catgtagcat gccggatcct gatgcagagc ggattttcag aagtttataa
    33061 ccttgccgga ggaatgaaga cttatgagac cgccacccaa aagcaaagta atgaagatat
    33121 cttttcgaat gattacatcg ggcacgacga cctgatctac cagggcagca aagaaggaaa
    33181 gccggttgtt gccgttaata ccattgatgt cgatgcctgt ggattgcagt gtccagggcc
    33241 tgtcctgaag ctcaaacaat cgatagaggc tcttgcagtc ggcgaagcag taaggattaa
    33301 agcatcggat gccggatttt ataaagatgt aaaagcctgg gccaacatga ccggcaacga
    33361 attggttgaa cttacccagg atggcgcttt gataacggct gtcgttaaaa aagccggaac
    33421 gccccaaaaa aagctggcac aatcaggcgg gaacaatgct accctgattt gcttcagcga
    33481 tgatctggat aaagcgctgg ctacttttgt gatagccaac ggagcggcgt ctaccggaaa
    33541 aaaagtcacc atcttcttta ctttctgggg attgaatgtg attaagaaac agaaaaaatc
    33601 tcctgttaaa aaagatttgt tcggaaaaat gtttggtata atgctgccat cctcgagcaa
    33661 aaaactgggc ttgtcaaaga tgaacatggc cgggatcggt gggcccatga tgcgcatggt
    33721 catgaaagac aaacatgtgg attcccttga aaacatgata caaacggcct taaacaacgg
    33781 agtggaattt atcgcctgcc agatgtcgat ggatgtaatg ggtgtaaaag ctgaagaatt
    33841 gatggagggc gttcaggttg gcggagtggc tacttatctc gaacgtaccg aaagtgcaaa
    33901 cctgaactta tttatgtgat gaatcaccag ataatactgt tcccgttgcc tgtttgatgc
    33961 tgcatgcatt tgcatcatta cggattattc ttccgtgaat gtttgcatca cagaggttaa
    34021 gcagaacatg gatatttgat ggattcctga aaattaagcc gttgaaaaaa atgcctgccc
    34081 cctgaaattc caaacggtgt gaactgcccc tgttgttttc acgattacct tccttccgca
    34141 gtatttgaat ccgcaagcct tattccatgc gattttattc agccggatat attttcctta
    34201 tctgcgggtt gcctgttgcc ggcggtaaca attcccttaa aaataaaatc gtccaccaca
    34261 tcaacgatgt tgccgctgac ggtaaatggg gcttcaataa aagcaaaatt ttaaaaatgt
    34321 acaaccgatt gttgtggtaa tgatggccag attcagattt ccggaattat tgaaatcagg
    34381 tcagtttccc ggatattttg ctttaatcgg acagattaac agaggactat tcctataagt
    34441 aaatttttga ttttgcaatt ttggattatt tcaactcaag acttctcagc gccacttctg
    34501 cacttcgcct gcagtcctgt tcgggattgc ggtttctcac cattcccatc gccttctgtt
    34561 tcattcactt ataagaatta tttaccagat aatctcatga aaatatatcc tttagttgtt
    34621 ataaccgcca aacattataa cttttagctg ttataacggc tgaatgtaat attctcacct
    34681 tctctgttcc tgaatttata actcctggct attgaaaatc aatatgatgg ctcgcatttt
    34741 tccccgctga ccggagttca tggccccggt ttattccaac ttaaaaccat gttgttcaat
    34801 ttcgctgctg agatcccgtt tggtcacgat ttccgccatt tcgctatatt tgctttcgca
    34861 agccttattc catgcgattc ttatcagccg gatatatatt cccgatcagc gggccgccta
    34921 ttgccggcgg catcctggtt atggacgacg aaggtttcgt ggcagaagtg ctcagacccg
    34981 gtcaggaaaa tctacctgat gaaaacattg agcctttcga cggtatcctg tgccccggct
    35041 ttgtgaacgc ccattgccac ctcgaactct cctgggccaa aggactgatc gaaagcggca
    35101 tcggccttga cggcttcctt cgcgccctcg acgaacttcg gcacaacgaa ctgcccgtag
    35161 atcctgttaa agctatcaag gaagaaggtg ctgaaatgca ccgctccggc gtagtagcgg
    35221 caggcgacat ctgcaactct gctctcacgg ttgcttttaa ggagtcgtcg gggatgctgt
    35281 ttcacaattt tgtcgaagtt ttcgcttccg atcctgccaa agcagaacct gccgcgatga
    35341 aaggcatcca gctggcgcag atattcaggg cgctccagcg gaacaacagg gcatcagtga
    35401 cgccccatgc tccctattcg gtaacggaac aacttttccg gctgatcggt ttcctgcctg
    35461 agaacacccc ggtcactatt caccatcagg aaagcgcggc agaaaaccgg ttcttcctga
    35521 acggttccgg ctccatggct gcacgccggc tctattacaa cccggggctg gcccctttca
    35581 ggccaaccgg aaggcggccg ttcgaaagca tcgcgccttt cctcaatccg gaacaaaaga
    35641 tactcctggt tcacaacacc ttttcggagc aggaagacat cggctttgcg cttcagcatt
    35701 ttcccggtgt agcctggtgt ttttgtccca acgccaacct tttcatcgaa agccggttgc
    35761 ccgatatccc gttgtttgtc aaaaatcatt gtaaaatcat gctgggaacc gacagcctgg
    35821 cttctaaccg ccggctttcg atcctggaag agataaaaac aatttccgct catttccctt
    35881 caatacctct gaacgagttg ctgacatggg ccacgcttaa cggggcggaa ttttatggct
    35941 tccgtcgcct gggatctttt gaaaagggga agaagccggg agtcgttttt atcaataacc
    36001 cggaacccgg atccatgaac ctgactcctg ccagcacatc tcgattaata attcccgcgt
    36061 aaccatgaga attttcataa accggaaaga aattacgatt tttgccggcg cccgggtact
    36121 cgatgcggtg cgggcttact cggtgcgatc ggcgaaaagg ctcctaaacg gttcgctttc
    36181 ggtcttcgac cgtttcggca acccgaccga acccgacggg cgtctaactg acggacagat
    36241 acttagcatc agaaaaacca aatagccatg aaaaaaatac tcctcccttt cattctttca
    36301 ctatcattct ttattgtaat tacgcagccg tccttctgcc agaaaaacaa tggcccggtt
    36361 gaagtcatta tcctgcatac caacgatatg catgccaaga tcgacaacat ggcgaaactc
    36421 gcctacctgg ccgacagcct gcggaaaaca catccctatg ttttcctttt ttcagcgggt
    36481 gataatttca caggaaatcc cgtggtagat atggttgccg acaaaggtta cccgatgatc
    36541 gacctgatga accgctgcgg tttcacactc agcgcgatgg gtaaccacga gtttgacctc
    36601 ggccaggaaa tgttcaacag aaggcaaaag caggcttgtt tcccgtttat cagttgcaac
    36661 atcgatgctt caggagccat cctgaaacaa cccaaacctt attatatcct gaaagccggt
    36721 aaaatccgca tcccggtact gggaattgtc gaactgggtg aaaacggttt acccgattcg
    36781 catcccagca ggctgggcgg tttgaagttc accgacggca tcgaaaaagc aaaggaattc
    36841 acctggctga aagacaaata tggcatgctg atcgcattga cccacctggg cgtggaaagc
    36901 gatgaaccgc tggccaaagc catgcctcag ttcgatgtga ttatcggggg gcattcccat
    36961 accaccatga ccactcccat gatggtgaac ggcgttatga tcgtccagac cggatcggga
    37021 ttgaaaaatg taggaaaagt aaccttgacc atcgaaaaca ggaaaatcac cagccgaaag
    37081 tatgaactta tacccctggc ttccatcagg aacggaaacc cgaccgtaca ggccgctatc
    37141 gataaataca acgacaatga agagctgaac cgggtaattg ctcaggctga aaacgatttc
    37201 aacggtgaaa acgcattggg cggtttgatg accgatgcca tcacatcaat gctttccaca
    37261 gatatggcat tccagaatac gggcggaatc cgcatctcct ccatcccgca aggtgaggta
    37321 aaatttaagg atgtataccg gctcgatccg tttgggaacg aagtcatcgt tttcaaaatg
    37381 aacctggctg agattcaatc gctgataggc aacgcctttg atcgcggcaa acaggttgat
    37441 ctgatccctt cgggagtaaa ctataccgta gtcacagata aacaaggttt gtgtaaaagc
    37501 gtcgaattgt cggatatcaa ggggaatccg cttgatcccg gcaaggagta cacggtggga
    37561 gtgaacagtt acatagctgc aagctataaa tttaaccacc gcgatgccgg aacatcccag
    37621 tataagacca cggcccagac gctgatcgat tacctggcaa aagaaaagaa catccggtat
    37681 tcaggcgtgg aacggaccac cgtaaaagcc ggtgaataga gccggactgc cattgcccga
    37741 cgtccggatt ggcagaagat atgacctgcc ggtacaatta taacaaaaag gcaaataaac
    37801 cggcgagggt ttcaaaccgg ctgtatgggc attacaaatt ggaaaagttt ctggactgaa
    37861 ttgtcggcag tttcaaggtg caagagccgg aatggctatg gctcctgaaa agttgtacct
    37921 cagtaactct cttgatccat cgatcagctt acctaaaaga atactttatt aataccgggc
    37981 cggctgacaa attcatgatc ctcatgctcc tgccaacaag tgagtaacga aaatgagggc
    38041 aatatgagaa tcatgaatag gaaaggtctc cgtatgcaaa ccatcagcaa acgctaagta
    38101 tttaaaatac aatttaatgc cattttttaa acatcaaaaa atgagataaa taagaattct
    38161 ccacctgatt atttcgaaaa aagtatagct ttgttcaaat ttattaattg gatcactgtt
    38221 ttttccataa aataaaatct aattctacgc ataattaccg tggggccctc tttcatactc
    38281 atttgatcaa ataaatatgt catcctatat gaataaaaaa atcatccttg caggaatatt
    38341 tctgagcctt ttctggcctg cacaagctca aatgaagaat ttgggagaaa taatggacaa
    38401 ttctaaccta ttgcttcaag atatccttta taacagcgat aattctattt tcggatacat
    38461 ctttatttat agtgtagata aggttaaaga gaaagactgg aaatgtgaat atctcatact
    38521 ggataaaaac ctgaatgaaa tagcccggaa tacttttatt caacacaagt acacttcacg
    38581 tacgataatc caattcattc aatgccggaa aaaaggcgat aacgttctgg ttacaatcgg
    38641 ctatgcatac acaaaagtat ttgcagcagc aaataactat ttcaattatc tgctcacgac
    38701 ataccgaagt atcagcttaa aggagaatgt tatctcagat gagttttatt atgacgacgg
    38761 ccagtttaaa acggttaatg ttacggatga caatctgtac aatatgtata aaaaggtcga
    38821 taatgtattt gagatatacc cgattgataa tgaacactat acaggatatc tggtacagga
    38881 aacctataaa gaagcaaaag atgaatacca gattaaagaa cttcgtgtta tcgatatcaa
    38941 taaaaatctg gtttgggcct ataaattcaa tcagtcagcg accagaaact cttacagcag
    39001 gataaccaaa atctattggc cggacgaaac gatcctgatt acattttcaa attttgagaa
    39061 ggacaaattc acaggtaaca gtttggttag tattgattta ctcaccggaa agaaactatt
    39121 tgagtatgtc gttgaggata aaaattcagc ttattaccat aacttcaaaa ccgaaaaata
    39181 tggtgataaa atttatctga caggtacata ttcccccaat gatgagcgtc gctatatatg
    39241 ggaagatcgg ataggatggt ttaagacaga actggacaaa gatggaaagc aggtgatgaa
    39301 aaaatatcat ccatggacag acgcatcgaa gtttatccag atcaaggaga atggaaaact
    39361 tgaagacgga taccggttaa actcaaaaga atttttaatt tttaaggatg ggtctgtaag
    39421 ttatctggcc gagaaattca atgaaaagaa aggagcatct gcatttgccg gatatgataa
    39481 gtcgaaaagc tcggatatgg tattggtaaa tttcgatgac aattttgaaa ttaaatcgtt
    39541 gaatgtcatt gaaaaagaag tttcaactac ctttcattat gattatttat tttcacaata
    39601 cattaagaat cacacaggtg cggtattctt ttttgaggat tataaaaaat cggatactga
    39661 taaaaagagt atatggtatc tggggattaa caagataatt gacggaaatt actcctatga
    39721 agaaatacca ataacttccc cgaaaaaaga atacgtgatt gttccatata ttgccaagga
    39781 aggttacatc atgctcaggg agtataacga aaaagataag ttcaatcaga tacggcttga
    39841 acgactcaat tattaaaaat accgctattt gcaaaccaac ctgattttaa tccggaaatg
    39901 agatattttt tgcttttact tgctcttctg ataatttgtg ttgcccccgc ccagcattac
    39961 attccaattg atacagccga tcatcagctc agagaaattc atatcaaaga atacaaaaag
    40021 gccgatgcgg tatttatttc agacatcagg aagaattaca gcggcaggga agagaaacaa
    40081 atggttgaag aactgcagga ctattctgat aattttattg aaaaaatcag ggaagggcaa
    40141 ttttgtttcg ataaaagatt tactgaaaaa gctgaaggaa tattatctga gatcaggaaa
    40201 gagaatacaa tggtccctgg taatatgaat attctggttt ccaaagaccc ctcactgaac
    40261 gcctattgcc ttccgaacgg aacattcata gtaaacatgg gagttttcta ctggcttcaa
    40321 aataccgacc agattgccgc cgttatttcg cacgaaatct ctcatattat cctgaaccat
    40381 cccatcagga atattttgaa tgccatcagg gatgatctgt cggaagaatc gagggagacc
    40441 ctgaagtcgg tccggaacca aaaatataac aagcgggaaa aagcgtttga attatttaag
    40501 cgaaagctgt atgccaaggg ggacattaaa cgaaagcatg aatttgaggc tgattcttta
    40561 ggatatatcc ttctgcgaaa tactgactat aattgtctgg actttattga agcactccga
    40621 ttaaacgaga agtatgattc tcttgtccct gatccttttc cgaatcaggt ttacagggac
    40681 gtgtttgatt tgccaaatca aaaattcagg gaagaatggc tgaagatgga agatttttcc
    40741 gcttatgact attccaaatt taaagaaagg tttgatcagg attcaatctc tacccatcct
    40801 gataccgaaa tcaggatcaa aaaactcgaa aatctgtttc ctgaactaaa caattccaaa
    40861 actaatccgc cggacagcgc atttgtgaca ttggcgaaaa ttgctgaata tgaacaggtt
    40921 cctgatttat attttgctga agaatatggt aacggtattt atttatgtct ggaaaggata
    40981 cgacagggaa aggatgatga tttttacaag gagtggctgg gtaaatgttt cgacaaaata
    41041 tatgaagcaa ggaagaatta tcggcttaac cggtatcttg atgccataaa tcccaaaacc
    41101 caacctttga attatcaaca gtttttaagc ttcatgtgga acctcagcct tgaagaaata
    41161 aaaaatatcc gggatttcta ccagccttcc gttcagaatg ttgaacaggt gaaaacggaa
    41221 taagatcatt atcgctgcgg atttttttta tcattttaag acggtttcaa tctgatattg
    41281 attctctcct ttccatcatt ttcctggaga tgatacagat atcatcaaac agccttttga
    41341 tgatatccgg ttttctgcca ctgatgcaag aagtgacaat attattgctg tcagaacata
    41401 aaacgtcgat attacagtat ctctcttaaa atcacctttc atttcagacc gttaatccaa
    41461 tagtaattca tacttttgta ctgtaagttt tacttttggt aaatcgttta ttatcaataa
    41521 cctgtactac aatatcatga aaaaagatac tgctgttttc aacatcatca ggaaagaaaa
    41581 agaacgccag atgcatggca ttgaactgat tgcctccgaa aacttcgtga gcgaccaggt
    41641 gctgaaagcc atgggatcgg taatgaccaa caaatatgcc gaaggttatc ccggacgccg
    41701 ttattatggc ggttgccagt ttgtggacca gactgaacag atcgctatcg accgcgccaa
    41761 gacattgttc ggcgccgaat gggccaatgt gcaaccgcat tcgggcgctc aggctaacat
    41821 ggccgtcctg atgaccgtcc tgaaaccggg tgacaccttc atggggctca acctttcgca
    41881 tggcggtcac ctttcgcatg gttcaccggt gaactcatcg ggtattcttt acaatgtaat
    41941 agcttacggc gtggaagaag aaaccggaac catcaattac gacaagatgg aagaagtggc
    42001 cttggaagga aagccgaaac tgatcatcgc cggggcatcg gcttacagcc gggattggga
    42061 cttcaaacgc atgcgcgcca ttgccgacaa aatcggcgcc atcctgatgg ccgatatcgc
    42121 ccatcctgcc ggactgattg ccagaggatt gctcaacgac cccattcctt attgccatat
    42181 cgtcacaacc accacccata aaacccttcg cggaccgcgc ggcgggatga tcctgatggg
    42241 caaggatttc gaaaacccct ggggcataaa gacgccaaag ggtgaaacca agatgatgtc
    42301 ggccatgctc gacagctccg tattccccgg agttcagggc ggcccgctcg aacatgtaat
    42361 tgcctccaaa gcagttgcct tcggtgaagc cctcagcgac gagtacatgg aatatatcat
    42421 ccaggtgaaa aagaatgccc gggtaatgtc gaaagcattt accgataaag gttacaaggt
    42481 gatctcaggc ggaaccgata accacctgat gctgatcgac ctgcgttcaa aattccccgg
    42541 aatcaccgga aaactggttg aaaacacact ggttaaagcc gatattacca tcaacaagaa
    42601 catggtcccg ttcgacagcc gcaccccgtt taccacttcc ggtatccgcg tcggcactcc
    42661 ggccattaca acccgggggt tgaaagaaga gcacatgggc gtcattgttg accttatcga
    42721 cgaagtaatc tctgatatcg aaaacgaagc cgtcatcgaa aaagttcgcg gcaaagtaaa
    42781 tgatatgatg agcgggttcc ccctgtttgc ctattgatcg tatcaaaata acgtgttata
    42841 attcaacggc gcttcaacca ggggcgccgt tttgttttct gcccgtgata tcctgaatgt
    42901 aaattggtta atgggcggca acttggcgta aattcgtaat aatttttact ttccgttgaa
    42961 atctcattaa aatgaaaacc gtcatccttg tccgccacgg caaagcgtcg cacgacgacc
    43021 tttcagtttc cgatttacaa cgcccggtca ccggcgaagg acgtaaacgg acaatgaaaa
    43081 tcgccaaata cctttcggca agggaaacgc atgttgataa aataatcacc agtcatgccc
    43141 taagggccac ggagaccgcg aaaatcctgg ggaaggaatt ggggataaca gagaaggatt
    43201 tcatcattga gaaagcaata tatggcgctc atcccgatac tttctacgat atcctgtttg
    43261 cattacccga cgatgtggat cgcgtcatga ttgttgggca caaccccggc atcgggatat
    43321 ttgcgtttcg gtttctgaaa acccaggtag aatcactgcc tacttcaggc gtggtggcgt
    43381 tgaggttcat caccgatgac tggtgcgaag tggcgtcgtg caggtccgaa gtggaattca
    43441 cggtgtttcc ttccatgata acataatgtt tccgattgca ttattgcatc cgatatgata
    43501 tatcgttatc tttgacttta atttccatac agaatgccca agaaaaaaac acttaccgtg
    43561 aaccgggaaa tttcatggct gcatttcaat gcacgcgtgc tgcaggaagc cattgatccc
    43621 gatacgccgt tgctcgaaaa aatcaagttt ctgggtattt actccaacaa cctcgacgag
    43681 tttttccgta cccgcgtggc taccctcaac cgcatgtcgg gaataaataa aaagctttac
    43741 ggcgaagtaa acatcagtcc gagaaaaacc ctcaaagaaa tcagcctgat tgacaagcaa
    43801 aaacagattg aattccaggg cattttccgc actatcgtcc atgagctggc caaaaacaat
    43861 ctgttcattc tcaacgaaaa agaactcacc aaggagcagg gggaatttgt ttcggcctat
    43921 tttcaggaaa atgtgcgccc gcaattgttc ccgattatgc tcggcaacct gaaagcggct
    43981 tcgctcgacg accgttcgca gtaccttgcc gttgttttgc aggttcgtaa caatccggaa
    44041 aaggaacggt atgccctcat cgaagttcct gtaggcccgc tgccgaggtt catccgtttg
    44101 ccggaagagg gcgaaaaaaa gtatttgatc cttctcgatg atgtgatccg ttattgcctc
    44161 ggcgatatct tcccggtatt cgggtacaat ttgttcaggg cctatacgat caagtttacg
    44221 cgggatgccg aacttgatat cgacagcgat atttcgaaga gttttctgga aatcatgagc
    44281 gagagcctga aacagcgtga tgtcggatca ccggtaagat ttacctatga ccgccacatg
    44341 cccgagccat tgctgaatca actgctttcc agaatgcaga tcagcaagcg tgatacggtt
    44401 ctccccggag gacgttatca taacttccgc gactttatga actttcccga tccgggaata
    44461 ccgcaactga cctttgaaaa atggccgccg ttgccgcacc ccgccctttc gggaaaggag
    44521 agcatctttt cgtgcatccg caaaggcgat atcatggttc attacccgta ccagtcgttc
    44581 catcacctta tcgatttgct tcgcgaagct tccatcgatc ctcaggtgag atctataaaa
    44641 atgacgctgt accgtgtcgc tacttcatcc aatgtggtaa acgccctcat caatgccgcc
    44701 cgaaacggca aggaagtgac ggttttcctg gaagtgcagg ccaggttcga tgaagaagcc
    44761 aatatcttct gggccgaaaa aatgcaggaa gaaggcgtcc gcgtcatcca gtccatcccg
    44821 ggtttaaaag tccatgccaa actgctcctg atccgccgcc atgaacccgg agagaaaaac
    44881 gtttactacg ccggcatcag cacaggcaac ttcaatgaaa caacggccag ggtttacgcc
    44941 gattcaacgc tgtttacagc taataaggaa atagcctcgg aagtgaatgc cgtttttcat
    45001 cttttcgatt ccaaatacag tgttccgcat ttcaggcacc tggttgtggc gcctttcagt
    45061 atgcgcagtc acttcatccg gcttatcaat aacgaaatac gaaatgcccg gaaagggaag
    45121 gaagcctgga tgctcctgaa actgaacagc ctggctgatg agaaaattgt aaaaaaacta
    45181 tatgaagcca gccagtccgg cgtaaagatc cgtttgattg tcaggggtat ctgtatcctg
    45241 gttcccggcg tttcaggact cagcgagaat attgaagcgg tgagcatcgt ggaccgctat
    45301 ctggaacaca cacgaatatt tgtgttctgc gacgggggca gcaacaagta ttatatttct
    45361 tcggccgact ggatggtgcg taatttcgat aaccggatag aaacggcatg tccggtttac
    45421 gacaaatcct tacaacagga attgatgtcg atgctgcaga tacagcttcg cgacaacgcc
    45481 aaatccagat atctcgggga gaaaaacatt aatgaataca aggaaaggga acccgggaaa
    45541 gagaagattc aatcgcagca ggagctttat aactatttcc ggaattatac cctgaagtaa
    45601 atctttttgt tctcccctat ccgtcacctc tctacgcgga cgctgtcgac caggtatcga
    45661 gattccccgc gccacggcaa caccccgttt ttctcttcgt aatgaatcac gatgaaatgc
    45721 ccctgaatgc tatcgagttg ccttactact ttttcgtttt tcaccgaaaa gaaaaacttc
    45781 tcggatgcca gcataatctc cttattgctt tgtacgctgc tcaggatgat ttcaccttca
    45841 taggtcttga aaatagtccc cttgcgtgaa aatttctgca ataagcccgc cctgtaaccc
    45901 tcactgtaag tgaagaaata tttccagtaa atgaaaacgg cgataaacag gcacaggata
    45961 aagataaacc agcgaaaaac acgctttaac cggcgcagaa aattattgat cggtttcccg
    46021 ggttgcatgc tccccgcact ggttggaact ggctggtttg tggtgtcggt catagcctgg
    46081 atttttaata tacaaacata gtaaaatacc gtgaaaaacg gatggcgttc ctgtaaattg
    46141 gattcatgac ttgtgacaat tggtgcggga acagggctgc tttattaacc actaaggcac
    46201 cgaggtcact aagtttcact aagaatagtg taatgctctg tttttcttcg tgttccgtga
    46261 ctctgtggta aaataccctt agtgcctaag tgttccatgt ctccgtggta taaatcttca
    46321 gtacccctaa gttttctact ctccgatcgc atcttctcct ctcgcatgcg ccaaggtaga
    46381 attatagatc agaataaatg gcgctcgaga ataagtgggg cacgtgtcag gagacgcgag
    46441 cctggag
//
LOCUS       DAOPYV010000016        44792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_694_length_44792_cov_12.848515, whole genome shotgun sequence.
ACCESSION   DAOPYV010000016 DAOPYV010000000
VERSION     DAOPYV010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 44792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44792
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_694_length_44792_cov_12.848515"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1362
                     /locus_tag="PKI35_03445"
     CDS             211..1362
                     /locus_tag="PKI35_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNX43468.1"
                     /translation="MKKVVLILTLILATGVLNAQSFSVLLDRIQALPAGEKTKLLDEY
                     FSGNHAVPVIENNHTVYFIYKGIASSVSVAGDATGWAPTLKMQKIAGTDYWVVSSQYE
                     ADARLEYKIVVDDTNWKLDPMNERVAHGGMGDNSELLMPQYEPAQYIYERDAVPWGTC
                     ADTVIYSWYLKQKRELRIYLPPDYKVTDALYPVIIFHDGFELFDRMAVRNVLDNMIFE
                     RKIRPVIAVFVQPVRRDGEYSGKLQRNYTNFITRELVGFIDANYRTVKSPDYRATAGI
                     SNGGNIALWLAASHPGMFGKAGAQSGNVEKNVLKAFGSQDLSASKIYLDFGKYDLPDL
                     VPKVRNLKKVLEERNIRHVYNEYPEGHNWGNWQKHLPEMLEYLFPRDGD"
     gene            complement(1372..2367)
                     /gene="trpS"
                     /locus_tag="PKI35_03450"
     CDS             complement(1372..2367)
                     /gene="trpS"
                     /locus_tag="PKI35_03450"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNX43469.1"
                     /translation="MDTVVSGIRPTGNLHLGNYFGAMRNFVKMQETNHCYFFIADYHS
                     LTTHPTPADLHGNVKQVLVEYLACGLDPEKATLYLQSDVPETAELYLFLNMNAYMGEL
                     ERCTSFKDKARSQPENVNAGLLTYPTLMAADIIIHKAHKVPVGKDQEQHLEMTRTFAN
                     RFNRMYKVDYFPEPVAYNFGEQLIKIPGLDGSTKMGKSEGEGNAIFLFDAPEVIRKKV
                     MRAVTDSGPVEPDQPKPQAIENLFNLMKALSAPETVRFFEEAYNNCNIRYGDMKKQLA
                     EDVIRFTTPLRERILELLGNDEYLRKVVNLGREKAHASASKTVKEVREIIGFKEF"
     gene            2698..4539
                     /locus_tag="PKI35_03455"
     CDS             2698..4539
                     /locus_tag="PKI35_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNX43470.1"
                     /translation="MKKLSILALICLSFIAGQAQVSVDRVEPPFWWVGMKEPVVQVMV
                     HGKNIGKTNVSVKATGVKIVKTQSPNADYVFIDLKIARNTKPLTVVFDFKDEGKTITT
                     WNYELKARENGSARRKSFSTADNFYLIMPDRFANGDPSNDDMPGMLEKANRNDPNGRH
                     GGDIKGITDHLDYLQKLGITALWCTPLLENNMPAYSYHGYAITDLYKIDPRFGTNENY
                     RDMVAEAHRKGIKVVMDMVFNHFGTEHWMMKNLPQKDWINQWPEFTRSNYRAAVVTDP
                     YASEYDLKRMSNGWFDKTMADFNQKNAFVANYLIQNSIWWVEYAGLDGIRQDTYPYAD
                     KDFMAMWMKRLREEYPDFRVVGEAWLNTPAQLCYWMDNTSNKDGYRSRLTNVFDFPLA
                     FAVQKAFSEEEGFDTGLRRLYDLLTMDVVYADPSDMVIFGDNHDIERMYQVQKTGENM
                     KMAIAFLCTTRGIPLIYYGTEALSDRGSLEGDAGKRKDFPGGWQGDKTNMFTGENLSQ
                     DQQEIYRYIKVLLNWRKGNKAVQEGKLTHFIPENGIYVYFRTLLNQSVMVILNNNKES
                     VKVNTERYNEMLDGYKKGVDAVTGATIKKLDKIDIDGKSARIIELSK"
     gene            4577..7408
                     /locus_tag="PKI35_03460"
     CDS             4577..7408
                     /locus_tag="PKI35_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNX43471.1"
                     /translation="MLLISFKNCHRFVLLLILAFSFRIGFVSAQAVKLVPENPSVTEK
                     ITIYYNAAEGNGALKDFTGDVYLHTGVITNKSIDGHDWKYVVGNWGTDDKRVLMKREG
                     PNLYSFSMQISSFYSLRDDDIARQLAFVFRNADGTMVGKTAENEDITVPVNGYKPPEQ
                     NQQTFTYKERNLMYLEKTSDGWKVHTDDGAIGIRPFTDEIIEVTYYPAEKYIPDSSHS
                     VVLQPAGVTLETDRLADGYRLRTGKLQACITNQPFRIIFVKDNDTLLKEEAGFFVNKE
                     NSGIRFRLNTTEMIYGAGERALPMDRRGYRLALYNRPFYGYEMGASMLNYSLPLILSS
                     KRYALLFDNPQKGYVDIGKGEKDIMEWGAIGGTLRYYFIAGGSWPEITGQYTQLTGHQ
                     PLPPRWVLGNLQSRMAYRNQQQAEEVVALMQQKKFPMDALILDFYWFGDSIQGHLGKL
                     DWYKPAWPDPSGMIGKFRKQGIKTVLITEPYIIDTVANYKDADAHGIFVTDSLGKTYH
                     DKQFYFGPGSLIDIFKPAARDWFWTKYDAQIRNGVAGWWGDLGEPESHPSDIYHVNGK
                     ADQVHNIYGHYWDKFLFDKYAEYYPDTRLFHLQRSGYAGTQRFAAFPWTGDVSRSWGG
                     FRAQLPLLLTMSLSGLAYIHSDAGGFAQGVRDEELYTRWLQFAVFTPILRPHGSNHPS
                     EPVFWSEKTQDIVRNYMNLRYAMLPYNYTLAWQNTVTGAPLMRPLFYQYPDDTTAMKT
                     GNEYLWGESFLVAPVTGKGQKSMKVYLPQGPWYDLNREGKYQGNQWLDYPVNLEQMPV
                     FIRGGSFIPFTDTISSTDYYDPSRYTVWYFPEGKTTYEQYEDDGRDNHALANGNYEII
                     RYEGNREPGKITVTIERKGQWKGMTGKKIITLQVPAIKAPQQVTLNGKTLKSGTGQSL
                     SAFSWSYDGHWISVKFPVGKKPVTVQIND"
     gene            7438..8814
                     /locus_tag="PKI35_03465"
     CDS             7438..8814
                     /locus_tag="PKI35_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNX43472.1"
                     /translation="MKKVVLLFIVPGFILAGFGSCKTNPAELKANYPEWAKNAVIYEV
                     NIRQYTPEGTFKAFEAHLPRLKELGADILWFMPIHPIGVKNRKMPEGMKESLGSYYSV
                     RDYKGVNPEFGTEEDFKNLVAKAHEMGFKVIIDWVANHTAWDNQWVTGHPEWYEKDST
                     GKILSPFGWADVAKLDYRNKDMRKEMIASMKYWIERCDIDGFRCDVAGEVPVDFWEQA
                     RAELEKTKPLFMLAENEDKPQLLMKAFDANYGWRMHQLMHDIAKGKDSVHSIVKQVLK
                     VDSVIPKNTFQINFITNHDENSWNGTAQEKFGPGENAFALLTYTLPGMPLIYSGQEAG
                     LNKRLAFFVKDTINWEGNDLSPFYEKLNALKHGNEALWNAPYGGRFELVKNTVPGKVL
                     SFIRKKGKNKVLVIVNLSNKYATVNLKGDKADGDYTDVFSGAAFTLNSSTLNLTLQPW
                     GYWVMEAK"
     gene            complement(8963..9379)
                     /locus_tag="PKI35_03470"
     CDS             complement(8963..9379)
                     /locus_tag="PKI35_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNX43473.1"
                     /translation="MEQTLSAEWKGNMKFEAQIDGHKITIDSSPELGGNDEGPRPKKL
                     MLLALAGCTGMDVVSILKKMHVELDSFDVDVIADITAEHPMHYTHMHIVYIFGGKDLP
                     MDKLQKAISLSQERYCGVSYMYRKAMEITHEIVIKQ"
     gene            complement(9554..11311)
                     /locus_tag="PKI35_03475"
     CDS             complement(9554..11311)
                     /locus_tag="PKI35_03475"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HNX43474.1"
                     /translation="MEIIRAHKPDDRWLKASVIGSYWAAFEIVAGSFLHNLKVPLSGT
                     ILASAGVFLLVSFVAVWKQKGLVWRAGAICALLKSISPSAIILGPMTGIMAEALLLEL
                     SICLLGRNLLGFLTGGALAVFSALLHKAVTLLIVYGLNLIKILEALYVFAVRQAGIPN
                     LQPGKLILLIVVLYLLTGALAALAAYLMASHSLENCGVSPEKKRLFLETESTLFDGKH
                     EHGYSLWLLMINIAAVPMALYLVNMAPAGLAVLAVTAYFVFVLTRYRNSTRHLKKVNF
                     WVQIVLITLIAAFLLNGLAGGEYFNFEGLKTGLIMNLRAAIILAGFSAISVELKNPVI
                     RAVTMRKGMAELYQSVNLAFSSLPAIFRMLPGARGIFRNPLKAFSGLFCYSQDLLSLF
                     ERQISNRPEIIVVSGQLHGGKTTYVTRVARELKSSGFSVTGFLAPAVRENGNRVGFDL
                     INIATGERTLLCREKGIEGIKYGRFIFHQNGLDTGNEILSPENLDGNQLVIIDEIGPL
                     EMNRQGWWKAVTELAETGKPQLWVVRSNLVQAAASNWKVGNVYVFDIEKDADGEVAGF
                     VKDLLQKEKNLSSFRTASG"
     regulatory      complement(11461..11594)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(11937..13175)
                     /locus_tag="PKI35_03480"
     CDS             complement(11937..13175)
                     /locus_tag="PKI35_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNX43475.1"
                     /translation="MHDPRHIAISDYDYPLPPERIAQFPLKQRDSSRLLVCKEGIITG
                     DVFSGLPDHLPAGSLVIFNETRVIHARLVFYKPSGSRIEIFCLEPFSPVNDVQLAFQQ
                     KGSCTWKCLVGNAKRWKGEILSLKATIDGSEVELKAEKGERLQDSFLVSFSWSPAEVT
                     FAGVLEAFGKIPLPPYIDREVIDSDNLTYQTVYARNDGSVAAPTAGLHFTPEVLAALA
                     AKQIECSTITLHVGAGTFKPVSSDTISGHAMHAEQVIIPVEIIEKLLNNPGRHITLVG
                     TTTARTMESLYWQGVKWLKHEAGSPAMLVEQWDPYKDEYQGVTLNESLSCVISTLQRF
                     RVKELKGYTSLLIAPGYEYHIPDAIVTNFHMPKSTLLLLVSAFIGDDWKKAYDYALEH
                     NFRFLSYGDSCLFFKKLRNF"
     gene            13334..13768
                     /locus_tag="PKI35_03485"
     CDS             13334..13768
                     /locus_tag="PKI35_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNX43476.1"
                     /translation="MTDKSKELITSLQLVNDKLHFEGTADGNPPVSIDYTPPLGDNLG
                     YTSLELLLLSLTSCIGSAILTFLRKMKKTINSIEINGRGFRKEEHPTGFKKIVVDIRL
                     TSPDVTEEDLRKVIKLAEDTYCPVWSMIKGNTEVETIFTIKN"
     gene            13910..14554
                     /locus_tag="PKI35_03490"
     CDS             13910..14554
                     /locus_tag="PKI35_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43477.1"
                     /translation="MNVQFGMARLLNCDEISNPGKFKFSKTISTGITIEYRIPVSKTV
                     NVEGQGFTAVTGIGANLLSYSIDKLLRDSTDIVISAINAGYDYNALQGFYFDIPLGIC
                     YITPYNDRHHNFSFELTSRTGFLVCSRSYIKMDTGGEKKEIVEKNTGLLNRFHTGFNA
                     KVSWNVMYLEPKKNELTGFRFSLWGEYYPTNVFPSGKGVSAQTSAAGVGIGFNF"
     gene            complement(14659..14919)
                     /locus_tag="PKI35_03495"
     CDS             complement(14659..14919)
                     /locus_tag="PKI35_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43478.1"
                     /translation="MTSSIFRQLPRMEQIQLLFDEGHELTGRFYLFFNIRLYTLKGLF
                     VEVWYHQVTNRIDRIMVVDSADVLTLYENQISIGDISNEIGR"
     gene            complement(14945..15670)
                     /locus_tag="PKI35_03500"
     CDS             complement(14945..15670)
                     /locus_tag="PKI35_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43479.1"
                     /translation="MDQLKPVESLGYILKKEKLASLASDINFKDLILEDLDQYPGFYD
                     QYFIPASEREKRPRSVFLILKEFDVCREDQFIRMTTKIKNRYKPDFDAALGVLQLFNT
                     PEPCIRVFMDDYSRVGELLSFYKQAGLQFQPHRTVPAYMSLIKIRKFFLMSPMAEGIY
                     KSMDQDDIYYFRIPGFLEWEDFERITIHIRNNWTHKIYDAAQVSIYDKSGMIEMVRIF
                     DLKGDLEKLSYLKLRYAHEIERL"
     gene            15828..16607
                     /locus_tag="PKI35_03505"
     CDS             15828..16607
                     /locus_tag="PKI35_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNX43480.1"
                     /translation="MTMSIRKHIPNSLTSLNLFSGCLGIVAVFEDNLPLAALLMAVAI
                     VFDYADGMAARLLHAKSDIGLQLDSLSDVVSFGVLPGFIVMKMMEKCSSLPDCAVSGI
                     NLCPFIALIIPVFSALRLAKFNIDTRQTDSFLGLPTPANAIFFGSLPLVIQQCNDNAW
                     TGLAGWLSNWGILTGLTLIFSGLLVSEIPLMSFKFKNLSWQSNKFRFIFLAIALLVLV
                     FLKFAGIPVAILLYIMVSLITGKQKSDNGHVSPVIKAGGKN"
     gene            complement(16630..17352)
                     /gene="lptB"
                     /locus_tag="PKI35_03510"
     CDS             complement(16630..17352)
                     /gene="lptB"
                     /locus_tag="PKI35_03510"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNX43481.1"
                     /translation="MLLRTDNIVKKYRQRTVVNNVTVHVEQGEIVGLLGPNGAGKTTS
                     FYIIVGLIKPLSGRVYLDKRDITDEPMYRRAQLGIGYLAQEASVFRTLSVEDNIRAVL
                     EFTGLSKQAQREKLEKLIVEFGLQNVRKSKGIQLSGGERRRTEIARALATDPKFILLD
                     EPFAGIDPIAVEDIQHIVSNLKTKNIGILITDHNVHETLSITDRAYLLFEGSVLKSGT
                     SKDLAEDEEVRKVYLGQNFELR"
     gene            complement(17401..19905)
                     /locus_tag="PKI35_03515"
     CDS             complement(17401..19905)
                     /locus_tag="PKI35_03515"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNX43482.1"
                     /translation="MKMKYLGECLPSRLQLSFLLTFAMSGLLLHAQEVTKITGTVTDA
                     STGQPMPFVNVYFKGSTVGATTGFDGKYSLESRKIADSLIASFVGYHTFSSAVKPNQY
                     QVIDIKLNPVNLDLPEVVIVPGVNPAEILLKKIIDHKPENDPARVDEYRCETYTKMQF
                     DVNNLSSKFQDRRVMKPFRFVFENLDTSVVNGKAYLPVMLSESVSDVYYRYSPRSRKE
                     IIKASKISGIENASMSQFVGSLAQDVNIYENFITLFQKNFPSPINSTGLLYYRYYLID
                     SATIDGHWCYNIMFKPRFKQATAFTGNLWANDTSFAVKKLEMRLVDDANVNFINDLVI
                     SQDFDKMGGKHWMLSKERMVADFNVFEDAKKTMGFYGARTAVFRNFSFDAPPDQKIYS
                     IPNDIIVLKDAAVQSKNFWEESRPEELTHREASVYLLADTLLKMPVFNTYFDVIKMVM
                     TGYYVKGKFEWGPYASMLSSNAIEGVRLRVGGRTSNDFSKRLMLEGYMAYGIHDERLK
                     YKGGFVYMLQKAPDRVISGSYKYDTEQLGMGEDALREDFFLSSVFRRNPQDKLSLVKE
                     IKGSYKHEWFTGFSNTLTFSNRNLYNIGNTGFSLYDPQGGSYEPHFSLITSELQFDLH
                     YGYREKVLAGEFERVVVSSPYPVLDLNYTYGVKGLFNGDYEYHKAQLRVTQWFNFLSA
                     GWSKYSVEAGKIWGKLPYPLLRVHSGNETFWFDEYSYNLMNYYEFISDEYVNAMYSHH
                     FEGFFLNHIPVIRKLKWREVAQARIAFGHTSDKNLQYNSFPTGTYALGKPYVEVGAGI
                     ENIFRFIRIDGIWRLTYNDHPDTNRFGVMVSMNFIF"
     gene            complement(19934..20275)
                     /locus_tag="PKI35_03520"
     CDS             complement(19934..20275)
                     /locus_tag="PKI35_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNX43483.1"
                     /translation="MGTKADEFNAYRQRMNERILSADDKVIKRIFNADTNAYAPGALD
                     VKTKEMLGLATSLVLRCDDCVRYHLLKCRENGVTEEELFEVLGIGTLVGGTIVIPHLR
                     RAVEFWDDIPR"
     gene            complement(20328..21317)
                     /locus_tag="PKI35_03525"
     CDS             complement(20328..21317)
                     /locus_tag="PKI35_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNX43484.1"
                     /translation="MPGSTIIVVKTAEEIRKIALDTIREESQAVENLARLVDDDFVKC
                     VQLIFNSPGRVIVTGIGKSANIAAKLVSTLNSTGTQAIFMHAADAVHGDLGMIGKDDI
                     VVCLSKSGNTPEIKVLAPLLKLMGNVLIAMVGNRGSYLAREADYIIDTTVEREACPNN
                     LAPTSSTTAQLVMGDALATALLECRGFTAGDFARYHPGGALGKKLYLRVSDVYIFNEK
                     PEVKPDDDIKTVILEISSKRLGVAAVIDGNKLAGVITDGDLRRMLHTHDSFVNLTARD
                     VMSVNPKTISCDALVSDALGVMRANNITQLLVMNNGTYSGVIHLHDILKEGVI"
     gene            21897..24116
                     /gene="recQ"
                     /locus_tag="PKI35_03530"
     CDS             21897..24116
                     /gene="recQ"
                     /locus_tag="PKI35_03530"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNX43485.1"
                     /translation="MRVKKVTEDYTDYPLHDLLKKIFGFESFKGNQEAIIRNVLAGND
                     TFVIMPTGGGKSMCYQLPAIIMPGTAIIISPLISLMKNQVDAIRQFGTDRGIAHFLNS
                     SLSKNEIAEVKEDLLRGNTKLLYVAPESLTKEENIAFFKDIVISFYAIDEAHCISEWG
                     HDFRPEYRRLRPIIESIGQDVPVMALTATATPKVQQDILKNLGMQEAALYKLSFNRPN
                     LYYEVRQKNEDVNKEVIKFIKMNQGKSGIIYCLSRRQVEELSETLQVNGIKALPYHAG
                     LDSPTRVTNQDKFLMEEVDVIVATIAFGMGIDKPDVRFVIHYDMPKSLEGYYQETGRS
                     GRDDGEGICLAFYSYDDIEKLEKFSKNKGVAEQEIANQLLMEVVSYAESSMCRRKQLL
                     HYFGEVYPEENCGNCDNCLHPKTKIDGREQVELALETILATKQQTKAKHIVNILIGKV
                     TSQIKQYKHNELEFFGKGMEGSERFWDGVLRQMLIERLVSKDIENYGILKLTPEGQKF
                     LKKPYSILVAQDSNPEEEGEDEELMAAGGARTSTTDKTLFSLLKDLRKEIARKENLPP
                     FVIFQDPSLEDMAIQYPINMDELQRITGVGAGKAQKYGKPFLELIANYVEENEIIRPM
                     DMVVKSVVNKSGLKVYIIQNIDRKISLEDIAIAKNLTSDELISEIESIVASGTKIDLN
                     YYLNENIDPYHQEEILDYFHEAETDSIQHALEELGENEYSEEEIRLVRIKFMSEVGN"
     gene            24203..24937
                     /locus_tag="PKI35_03535"
     CDS             24203..24937
                     /locus_tag="PKI35_03535"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNX43486.1"
                     /translation="MTENITSEDTHNEIENPVEPITEPSQPEAERQPGKHPRKTDKLQ
                     LLLAVNILFFLALAVLYVLFFSNRQGGNSESMAKTLQKTGKGNMSVAFVNNDSILAHY
                     DLVTKLSDELAGKTKRLEGEIAAKQKAFEKDASYFQDQVQKKALSDQSAQEIYGQLQQ
                     NQQGILELRDRYAAELQQNQLDMNVVVLDSVMNFLKRYNEKYKFDYILGFTKGGNILY
                     ANDTLDITNDVIKELNEQYHKKNPAK"
     gene            24950..25417
                     /locus_tag="PKI35_03540"
     CDS             24950..25417
                     /locus_tag="PKI35_03540"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HNX43487.1"
                     /translation="MYQRIVKIILVFLPVIMFSQACDKVEGVFSSRPSGVLSKKEMTS
                     MLVDIHLQEAVIRTGVHTGTDTDMRNYSRSGYLKVFAKHDVTPEEFKKSLDYYLLHVD
                     ELDDIYTGVINQLTQMQAEAQGEKGAPAAKSPAPGADTAGHNVKKVPDMPAGH"
     gene            complement(25446..25874)
                     /locus_tag="PKI35_03545"
     CDS             complement(25446..25874)
                     /locus_tag="PKI35_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43488.1"
                     /translation="MKTYTRNLFILLLLVMMAITSCEIAEDEPGSDDPRDKMTGTWQF
                     SENSVKSTRSISYTVNITKDPDNSSQVILENLGNPGSFDIQSSGIVTSSQIVVSEQTL
                     SNGWVIEGSGKLVSSGTMNWTYSLIAGGDIEEYTAVATKL"
     gene            complement(25942..26748)
                     /locus_tag="PKI35_03550"
     CDS             complement(25942..26748)
                     /locus_tag="PKI35_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNX43489.1"
                     /translation="MIRKLLTPVFLVFFSGLLSAQTPLDTAVNFTVKDVKGITHHLNN
                     YLEQDKLVVADFFTVTCGPCVTYAPEINAAYEYFGCNTGNVIFLGINWGANNRQVTEF
                     CDAYGAYYPAASGLEGNGNHVVNDYQLLSYPTVILIKPGGAIAENYIWPPSQQHLDSV
                     ITSCGGISMPCNTGIQEAPETNDPVLKVFPNPATGSVSIQTSLSGDLTLSLVSVMGSA
                     VRQEEFLRSPSDPYILNLTGVLPGYYTVIIRSHGTIVGSSPLLVGRTGCR"
     gene            complement(26833..27645)
                     /gene="deoC"
                     /locus_tag="PKI35_03555"
     CDS             complement(26833..27645)
                     /gene="deoC"
                     /locus_tag="PKI35_03555"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNX43490.1"
                     /translation="MDQYLKAITEPFSKGKSRIDALTQILGFLDLTTLEGTDNKSRVN
                     ALCDKALSFSDKGTPSPAAVCVYSPFVRLVTDRLKGSGILTASVAGAFPSGQLPIHLK
                     VAEVGYAVSEGADEIDMVLSRGTFLAGDFSTIRSEVAAMKKACGKAHLKVILETGELA
                     TAENIRLAGELAIEGGADFIKTSTGKTQPAATPEALAVMSDVIREHFSATGKQVGLKP
                     AGGISTPDQALAYYAVVQDKLGDAWLNKNLFRIGASRLADAIFAELEAWKNH"
     gene            28100..>33516
                     /locus_tag="PKI35_03560"
     CDS             28100..>33516
                     /locus_tag="PKI35_03560"
                     /inference="COORDINATES: protein motif:HMM:NF039230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNX43491.1"
                     /translation="MVLLIPSLKMNAEGTRQLEPSTGPATQYCKMYFDNDLGQFRIPF
                     AQLGCPSDYRLYFYIDYPATEVVYFGFGSAIDYGNIAHNDLQFQIKDPSDNVVSGFTM
                     APLPVAGAGFISTNAQAQAGPNISGSVPAGYTPLSFTPTMSGNYYIEFSMNNLPDDIN
                     GRGLRYFDLTVANGTTPIDGRLWCKAWQLSSSTTSAGATGKTFASFYSYTADSIVTRF
                     QCNGLAGGIFSVYCNQYGTNNTGNWATDRRSINGNGTVIPEYRLFLNNPDTNIYPSGH
                     LGRICDVSSHPECDGSVDILIKVNRPGTIFVDIDLLPAGAGPEDITLIKAVTGSANCD
                     TWDTIPWNGTNGLGVAVQNGVINDMTIVYLNGLTNLPLYDVEDNRFGIKVDLIRPTVT
                     GIHKMPIYWDDVNVGGSTNLTGCTYPAGGIVTGCHPWTNTNMLTHNSWWYYLTDGPEN
                     LTVAVYRTPPAAPSPTGPSQVCQGQTSVTFSIPAQQSTDLYIWNLPDGSVDTTLVPSV
                     TFDIDSTAAASGTISVILNNSNCGNGAASAPFSISVTQTPNPSPNLIVRPCPNKTGDL
                     TTVQPLAAAGTSLQWHTSATSPSGATLVSTPSAVPSGTYYLFAVNSTTGCFSPASPAV
                     VVSCDTCPPVAVTDSLFTSNTLAVTVNILTNDFAGDYAINPATVDLDPATPGFQQTIT
                     IPGQGTFTVNSIGVLTFTPVSSFVGIATVPYTINDIYANTSNQASVIVTVGPDGVSVP
                     AYQIICSGDTAIIHLSSSIPGATFTWTVSGPASVTGQTPGSGNLISQILVNNSMSNHT
                     VTYFVTPWYGGIAGPVVTAQVEVTPPVTLTNATLLKTICSGDNTNINLNTSPNNATVS
                     WVASNTGNISGIVPASGTTKAINQTLINNDPFANYVYYNIEVSYYGCVTDPETYTVTV
                     NQDPDVQVSPASSVICSGTSVNLVLTSTIPGTTFSWTASGTAGVSGYSAGNGNTISQA
                     VSTAASVPGTVTYTITPTANGCAGTPVNYQVTVNPVPSVSNPSLAETICSGSSTAISI
                     TSNIAAATFTWTASGSAGITGYSAGVGNIIAQTLDNPGNIPGTVTYSITPSAYGCTGS
                     TVDYIVTVNPVVNVTTSPLNQSVCTGYSTLIPLTSNVAGATFSWTASGTPGISGYSAG
                     SGNVISQVLTNSIATPGTVTYTITPAANGCTGVSSSNFIVTVNPEPVIFTMVPTGPQC
                     SGTILRLNGSESGLLYILRLNGISVDTITGTGLVGFLDFGPRYDAGIYDIIAVNPVTG
                     CQALMNGLVQMNPAPDIFSVLPAGILCPGANIGLSGSETGISYQLRWNHMINIGSPVS
                     GTGSPISFGTQIFPGTYTIIAVNPATGCYSEMADSATIYPQPTIYTLTANGTGCPGTS
                     IGLSSSQLLIDYVLLLNGTIHLDTVSGTGNPIDFGPQTTAGSYTILAISQSNFCQILM
                     NGTYVINPDPLVYNLTPAGYICAGQSIGLSGSETGISYQLYNGATLVGSPVAGTGSAL
                     DFGPQTTAGVYTVIATNPATGCYLQMANSVTVNPNPVIYSLTSLGAGCPGTTVGLNGS
                     ELNTNYILVLDGTLYLDTISGTGNPVDFGPQTTAGTYTILAVNQNSFCQSLMNGSLVM
                     NPNPSVFSLTPAGYICAGQSIGLSGSETGISYQLYNGTTLVGSPVAGTGSALDFGPQT
                     TAGVYTVIATNPATGCYLQMANSVTVNPNPVIYSLTSLGAGCPGTTVGLNGSELNTNY
                     ILVLDGTLYLDTISGTGNPVDFGPQTTAGTYTILAVNQNSFCQSLMNGSLVMNPNPSV
                     FSLTPAGYICAGQSIGLSGSET"
     assembly_gap    33517..33616
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <33617..41118
                     /locus_tag="PKI35_03565"
     CDS             <33617..41118
                     /locus_tag="PKI35_03565"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX43492.1"
                     /translation="PAGYICAGQSIGLSGSETGVSYQLYLGTTTVGSPVAGTGSALDF
                     GPQSITGVYTVVATNPATGCYSQMSGAVNIYPQPTVFTITSTGSGCPGTVIGLSSSQL
                     NVDYVLLLDGSVQIDTISGTGNPIDFGSQMTTGTYTILAISQSTNCQEMMSGSFVINP
                     NPFVFNIVPAGTICVGQSVGLSGSETGVSYQLFNGTTLIGTSIAGTGNALDFGPQWIA
                     GTYTVIAVNDVTGCASEMNDSTVFRPFPTSYAIYPQGTNCSGSNIILNGSESGITYIL
                     MLNNTFPVDTLTGTGGILDFGVQYLSGIYTVNASATALTCQSTMSGSTIIVNNPLVYN
                     VIPAGTNCGTANIGLDGSETGVNYELFLDGNPFGIVVAGTGSPVSFGNQYPGTYTVQA
                     VNTVTGCQSAMNGTIVINELPAADAGADDIICENSIAQLSGDAFEYSTVLWTTAGDGT
                     FINPTSLYADYIPGVNDIANSVVTLTLTASNPLCGSASDDMLLSIIPYPVVDAGPDAT
                     VCDACDFHIIYATATNTSSVYWITSGTGTFSDAASLNPVYFPSETDYAIGSVILTLTG
                     TGIDMCGEANDIMTLTFDPNPGVDFTWGASCESQSVDFAVDPAATNIGAVASWSWDFG
                     DGGTSLLMNPSHVFPSWGTYNVKLTTTDTTGYVRIVMNEVYVSQPPVAMFSSSVSNCS
                     NEEVQFKDLSFTLYGYIEQWVWNFGDGTPDDTINFPDDPNVKHMFDTTGIYNVTLTIR
                     NSFGCENSVILPVTVITAPIANFYYTGNCASTVVNFSDASFANGSGNVVQWWWNFGDP
                     LSGSDDNSELENPIHTFTEPGTYMVTHIVRNFNNCTDTIVKPVVILPPLDVDFTHTYT
                     CIDELTHFIPDTTVIAAGAVQSWFWDFGDGLTSTDMYASHAFTSTGLYHVTLTITDTN
                     NCVASGSHDITIYALPIANFETEPWLCEDTPVEFTDLSSAASGYIIKWFWDFGNGDTL
                     TVNYPDNPNIQYTYAIPGTYNVTLSIKSTDSCTAEITKSVIIGSTPVANFTFDNNCED
                     NLTQFTDLSQLNGGGNITGWAWNFGDPASGILNTSTVQNPAHQFASTGVYNVTLTVTT
                     SNGCANEVILPVTVIPAPTVDFYAQQTCAGTSTLFIPASTMAIASVETWFWNFGDGST
                     SNEQDPGHIYPASGNYLVTLTITDTNGCSNTAAHNIAIAPAPTANFITSQPSCSGSAI
                     SFTSLAATQLGYIVKWTWDFGDGASQTITLPGNPNVSHIYSDYGLYNVTLTVVTNDSC
                     QGSTSLPVQVLQSPVANFSSDGSCIGSPVHFNDLSQGGMILTWHWNFGDMYAGPANTS
                     VLQNPQHTYTSAGTYPVTLITENVNGCSDTIIRNVLIYAAPAVDFTFNASCANDTVHF
                     ISSASVNVAATAAWLWQFGDGATSTDTDPYHVYAAQGYYNVTLTITDTAGCENSKTRN
                     VPVTQAPVAAFTATTPACSGMSVTFNDISTTMNGTISSWHWNFDDGSDTTIFAPANPD
                     LEHVFAQAGTYNVSLYITTTTGCEDEYSLPVNIKFSPAAAFSVQGNCPVQPAMFTDES
                     QALGGSAIVAWSWSFGDPASGSNTSVLQNPQHIYSQPGTYNVTLEVENMNGCTNSITQ
                     PVTVETPSAVAIADIMTSCLGTPVVFQVDTTVTNLADITAYDWDFGDGTAHSPMANPS
                     HDYTNAGTYTVTLVITDINGCTNSDSQPMTVHTLPVAQFSSQSNCLESYTVFSDESYV
                     PDGDPIVSWNWDFGVTGSTNDTSSLQNPVWIYAGTGTYQVTLQVSTLSGCTATVISEV
                     TVKPAPVANFTYITEPCHQGSVSFTDSSYAQQSVVTNWYWEFESNNYSTLQNPTYVFM
                     NTNTYYDVKLIITNAEGCTDTIIKPVYVPSGLNMTFSFDETCFGDTTRFTPQLLPPVL
                     DSIATYAWSFGDPNTGIYNQSNLRYPTHKFLKAGTYVVTLTVTTTSFCTATVNRQIEI
                     IALPNPKFEYTGGDCNDVVEFQDKTSGNNLTQWIWEFGDGTSMTINAPSSPDVTHSYP
                     NTGLYFATLTVTNAHGCVNAVTDSVKRLPCISSEFTINDTTACQNRSMHFTDMSTCAG
                     PIAGWMWDFGDGTTQTYTSQQAVVSHTYATAGSYTVRLIVNTQLVGGIIAADTSTKPL
                     KVFPAPKAEFIWNDICLGTTTEFKNTTRENGLKVNDYTWNFDDPTSIFDTASMISPTY
                     LYSEAGGYNVRLVAGNTMGCTDTIVQTVNVFDSPKAAFDWLTSCDGDPVQFIDNTDTA
                     SAKISTWNWIFHDEAGAVIGASTKQNPSFDFTRAGLFSTELSVKDKHGCTGNTVNQIA
                     INSSPVAAFTIKDNYEDTQGQIQLLNGTLNATNYLWDFGDGKTSFSESPVTTYDKGGS
                     YTITLVTWNGQNCTDTLTMNYELTFKGLYIPNAFSPDDPHQEVRIFKPVGMNLKKYRI
                     EVIDRWGNTLWTSDKLDKNGCPAEGWDGTLNSVPVQSGVYMWRASAIFIDGTKWDGVN
                     VGDKTCLPQTDTGTVTLIR"
     gene            41243..42364
                     /locus_tag="PKI35_03570"
     CDS             41243..42364
                     /locus_tag="PKI35_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNX43493.1"
                     /translation="MKRYSLTCNITAHVALILLALVSVLSLKAQDPHFTQFYNNPIYY
                     NPGYIGLNPGYRARFNYRDQWTGLPTDFKTYSFSMDVAERGIPGSGGLGMLVLSDKAG
                     TGLIKNNSVGIGTAARVPLQENMVTQVGFMVSFVQKSIDWDHLVFTDQLSARYGNIYP
                     TNFEAPDKNNVTYPDFSVGGVYRFAETGWNFSAIQGTFGLALHHAFTPNESFLGLTAT
                     LPRKLVVSGDLVLEIEQGRGSSYRNYRNTGSFKFNPGFIYEKQAEFSTYSVGVNVLKS
                     SIYFGIWFRNQTFDLFKANDAMFSVGVNAPFNRESRLKIMYTYDYIITELRTAARASH
                     EISLVFEFDSFSLFSNGGGGGFAPSYRGSKFREMECCPF"
     gene            complement(42448..43329)
                     /gene="era"
                     /locus_tag="PKI35_03575"
     CDS             complement(42448..43329)
                     /gene="era"
                     /locus_tag="PKI35_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNX43494.1"
                     /translation="MTHKAGFVNILGHPNVGKSTLMNLLIGEKLSIITAKAQTTRHRI
                     LGIVNGPDYQVIYSDTPGILKPHYKLHKSMMKFIDNALQDADVFLYMTEVGENTFEED
                     VIRKIQASGKPLILIINKVDLASPEEAEQKVVFWKEQFPGAEVIPIAAKLNYNIGKIS
                     ETVLQLLPENPPYYPKDDELSDRPLRFFISEIIREKIFLQFTKEVPYSTEVVVESYKE
                     GEKMTVIAATIFVERETQKAILLGHKGAAIKKLGIDARKDIEVFIGTKVYLELSVKVS
                     DDWREDERQLRRFGYEL"
     gene            43426..43497
                     /locus_tag="PKI35_03580"
     tRNA            43426..43497
                     /locus_tag="PKI35_03580"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43458..43460,aa:Met,seq:cat)
BASE COUNT        11864 a        10850 c        10448 g        11530 t
ORIGIN      
        1 aggcctatca gaacggcttt ttcgggtttc agatcagaag tgagcattat ttatatttgg
       61 actgcaaagt taaaaatcct cttcatctgc aaattgctct gttgaaaggc ttttctgact
      121 gcatgacaaa tgatgaaata ggatgcctga cggttttagt tttaacagta atcggtaaca
      181 agttacattt gtatacaaaa caacctgaac atgaaaaaag ttgtactgat cttaacgctg
      241 atcttagcga ccggtgtcct gaacgctcaa tccttttcag tgttactcga tcgcatccag
      301 gctttgccgg caggagaaaa aacaaaactg cttgacgaat atttcagcgg gaatcatgcc
      361 gttccggtga ttgaaaataa ccataccgtt tacttcattt acaaaggaat agcttcttcg
      421 gtaagtgttg ccggcgatgc aacgggttgg gcgccaacct taaagatgca gaaaatcgcc
      481 ggtaccgatt actgggttgt cagttcgcag tacgaagctg atgcccgcct tgaatacaaa
      541 attgttgttg acgataccaa ctggaaactc gacccgatga acgaaagagt cgcgcatggc
      601 ggcatgggcg ataattcgga attgctgatg cctcagtacg aaccggcgca atatatttac
      661 gagcgtgatg cagttccctg gggtacttgc gccgatactg tcatctacag ttggtatttg
      721 aaacaaaaac gcgaattacg tatttacctt ccgccggatt ataaagtgac cgacgccctg
      781 tacccggtaa ttatctttca tgacgggttt gagctgttcg accgcatggc cgtccgcaac
      841 gtgctggaca atatgatatt tgaaagaaaa ataaggccgg tgattgcggt ttttgtgcag
      901 ccggtccgtc gcgacgggga gtattcgggg aaattgcaga gaaactatac aaattttatt
      961 acccgggaac tggtgggctt tatagatgca aattaccgta ctgtgaaatc tcctgattac
     1021 agggcaactg caggtatatc gaatggcggc aatattgctc tgtggctggc ggcctcccat
     1081 cccggtatgt tcggcaaagc cggggcccag tcaggcaatg tggagaaaaa tgtgctgaag
     1141 gctttcggct cccaggatct ctccgcctca aaaatctacc ttgatttcgg caaatatgac
     1201 cttccggatc ttgttccgaa agtccggaac ctgaaaaaag tacttgagga aagaaatatc
     1261 cggcatgttt acaacgaata cccagagggg cacaactggg gcaattggca gaagcatttg
     1321 cccgagatgc tggagtatct gtttccgagg gatggagatt agagagactt cttaaaactc
     1381 cttaaacccg attatctccc ttacttcttt tacggttttg gaggcgctgg catgggcttt
     1441 ttccctgcca aggttcacta ccttgcgaag gtactcgtca ttgccaagaa gttccagaat
     1501 cctttcgcgt aacggagttg tgaaccggat gacatcttcg gccaactgct ttttcatatc
     1561 gccgtagcgg atattacaat tattgtaagc ttcttcaaaa aaccggactg tttccggtgc
     1621 agataaggct ttcatgaggt tgaaaaggtt ctcaatagcc tggggtttcg gctggtccgg
     1681 ttccacaggg cccgagtcgg tcacggcgcg cattaccttt ttgcggatca cttccggcgc
     1741 atcgaacagg aatatggcat tgccttcgcc ttcgcttttc cccattttgg tgctgccgtc
     1801 caggccggga atttttatca gttgctcgcc gaaattgtag gctaccggtt cagggaaata
     1861 atccacttta tacatgcggt tgaaccggtt ggcaaaggta cgcgtcattt cgaggtgttg
     1921 ctcctggtct ttccccacag gtaccttatg agccttgtgg ataataatat cggctgccat
     1981 cagggtaggg taggtcagaa ggccggcgtt cacattttcg ggctggcttc gtgctttatc
     2041 cttgaaggaa gtacaacgtt ccaactcgcc catgtaagca ttcatgttca ggaacaggta
     2101 tagctcggcg gtttcgggga cgtcgctttg cagataaagc gtcgcttttt caggatcaag
     2161 gccgcaggcg aggtattcca ccagcacctg ttttacattt ccgtgcaggt cggcaggggt
     2221 aggatgcgtg gtaagcgaat gatagtcagc aatgaaaaaa taacaatgat tggtttcctg
     2281 catcttaaca aaattccgca tcgccccgaa ataattcccg aggtgcaggt tgcctgtagg
     2341 acgaattccg cttactactg tatccatgtt ttctacgtca gattgattat caataaaatc
     2401 accggcattt aagccggtaa ttgtaattct tctgcaaaaa tagtgaaaag agagttataa
     2461 ggagaaaccg cctggcggca ggtaaccata cagccaatcg ggacctcttt gaatacgttt
     2521 ctgcatcaaa tcccgacaag ggaaggaaag caagagaggc gaattaaatc aggaggaaac
     2581 cggcggactg ccggccgctg ttacattaat ttattctcct gcaaacgttt gcgtagcagg
     2641 tccatttgaa tatccggatt cttttcttat atctttgcag atatcaaatt atttctcatg
     2701 aaaaagttat cgattctggc attgatatgc ctgtcattta tagccgggca ggcccaggta
     2761 tccgtcgacc gcgttgaacc acccttctgg tgggttggaa tgaaagagcc ggttgtacag
     2821 gtaatggtac acggtaaaaa tatcgggaaa accaatgttt cggtcaaagc cacgggagtg
     2881 aagatcgtta aaacgcaatc cccaaatgcc gattacgttt tcatcgacct gaaaatagcc
     2941 cgcaatacca agcctttgac cgtggttttt gatttcaaag atgaaggtaa aaccatcaca
     3001 acctggaatt atgaattaaa ggcacgggaa aacggttcgg cccggcgaaa gagtttttcc
     3061 actgccgata acttttacct cattatgccc gaccgctttg ccaatggtga tccatccaac
     3121 gacgatatgc cgggcatgct tgagaaagcc aaccgtaacg atcccaacgg acggcacgga
     3181 ggcgatataa aaggaataac cgaccatctc gactatctgc agaaacttgg tattactgca
     3241 ctctggtgta ccccgttgct cgaaaacaac atgcctgcct attcctatca tggctatgcc
     3301 ataaccgatt tgtacaaaat tgatccccgg ttcggcacca acgaaaatta ccgcgatatg
     3361 gtggccgaag cccaccgcaa aggcatcaag gttgttatgg atatggtttt caatcatttc
     3421 ggcactgagc actggatgat gaagaacctg ccccaaaagg actggataaa ccaatggcct
     3481 gaattcaccc ggtcgaatta ccgtgccgcc gtcgtgaccg atccctatgc ttcggaatat
     3541 gatttgaaaa ggatgagcaa tggctggttc gataaaacaa tggccgattt caaccagaag
     3601 aatgccttcg ttgcaaacta cctcatccag aacagcatct ggtgggttga atatgccggc
     3661 ctcgatggca tccgccagga tacctatccc tatgctgata aagatttcat ggcaatgtgg
     3721 atgaaaaggc tgcgcgaaga ataccccgac tttcgcgtcg tcggcgaagc ctggctgaat
     3781 acgcccgccc aactgtgtta ctggatggat aacacaagca acaaagacgg ttacaggagc
     3841 cggctcacca atgtttttga ttttcccctt gcctttgccg ttcaaaaggc cttcagcgaa
     3901 gaagaaggct ttgataccgg cttgaggagg ctctatgacc ttctcaccat ggatgttgtt
     3961 tatgccgatc cgtcggatat ggtcattttt ggcgataatc atgatatcga acgaatgtat
     4021 caggtacaga aaaccggcga gaacatgaaa atggcgatag catttctttg cacaacacgg
     4081 ggaatcccgc ttatctatta cggaaccgaa gccctcagcg accgcggttc actcgaaggc
     4141 gatgccggta aaaggaaaga ttttccggga ggatggcagg gtgataaaac caatatgttt
     4201 accggtgaaa acctttcaca ggaccagcag gaaatatacc ggtatatcaa agtattgctg
     4261 aactggcgca aaggaaacaa agccgtacag gaaggtaaac ttacccactt cattcctgaa
     4321 aacgggattt atgtttactt caggacgctg ttgaaccagt cggtcatggt gatcctgaac
     4381 aataacaagg agagtgttaa ggtaaatact gagcgttata acgaaatgct ggatggttat
     4441 aaaaagggtg tggatgctgt aaccggggcc accataaaaa aactggataa aattgatatc
     4501 gacgggaaat cggcaaggat tatcgaatta agcaaatgat gattttccgg cattatcaaa
     4561 ctgatatctg acctctatgt tgttgatttc cttcaaaaat tgtcatcggt tcgtcctgct
     4621 gttgatcctg gcattttcat tccggatcgg gttcgtttcg gcccaggctg taaagctggt
     4681 tcccgaaaat ccttccgtca ctgagaagat cacgatttac tacaatgctg ctgaaggtaa
     4741 cggggctctt aaagatttta cgggagatgt ctacctgcat accggcgtca ttaccaacaa
     4801 aagcatcgac gggcacgact ggaagtacgt tgtaggcaac tggggaaccg atgacaaacg
     4861 tgtgctgatg aagcgcgagg gcccgaacct ctactctttc agcatgcaga tcagctcttt
     4921 ttactcgctt cgggatgacg acattgcacg gcaactggcc tttgttttca gaaatgccga
     4981 tggcacgatg gtgggaaaaa cggctgaaaa cgaagatatc acggttccgg ttaatggtta
     5041 taagccgccg gaacaaaatc aacagacatt tacgtacaag gaacgtaacc tgatgtatct
     5101 tgaaaagacc tctgacggat ggaaagtaca taccgacgac ggtgccatag gtattcgacc
     5161 gtttaccgac gaaatcatcg aagtaactta ttatcctgct gaaaaataca ttcctgattc
     5221 atcccattcg gttgtactgc aacctgccgg ggtaacgctt gaaacagatc gccttgctga
     5281 tggataccgg ttaagaaccg gtaaattaca ggcctgcatc acgaatcagc ctttccgtat
     5341 catctttgtg aaagataatg ataccctgtt gaaagaagag gctggttttt tcgtgaacaa
     5401 agagaattca ggaatacggt tccggcttaa tacgaccgaa atgatatatg gggccggcga
     5461 acgcgccctt cccatggacc gccgcggata ccggctggcg ctttataacc gcccgttcta
     5521 tggttatgaa atgggcgcct cgatgctcaa ttattcgctt ccgctgatcc tttcatccaa
     5581 acgttatgcc ttgctgttcg ataacccgca aaaaggatat gtggacattg ggaaggggga
     5641 gaaagatatt atggaatggg gagcaatcgg ggggacttta cgctattatt tcattgccgg
     5701 cggctcctgg cccgaaatca cagggcagta tacgcagctc accggccacc agcccctgcc
     5761 gccccgatgg gtattgggta acctgcaaag ccgcatggca taccggaatc agcagcaggc
     5821 tgaagaggtc gtagccctga tgcagcagaa aaaattcccg atggacgcgc tcatcctcga
     5881 tttctactgg tttggcgaca gcatccaggg acatctcggc aaactggact ggtacaaacc
     5941 agcctggccc gatccgtccg gtatgatcgg gaaattccgc aaacagggga taaagaccgt
     6001 acttataacc gaaccctata tcattgatac cgttgcaaat tataaagatg ccgacgccca
     6061 tggcatcttc gtcaccgaca gcctggggaa gacataccac gacaaacagt tttatttcgg
     6121 gccgggaagc ctgatcgata ttttcaagcc tgctgcccgg gattggttct ggacgaagta
     6181 tgatgcgcag attagaaacg gagttgccgg atggtggggc gacctgggcg agcccgaaag
     6241 ccatccttcc gacatatacc acgtaaacgg aaaagccgat caggtccata acatctatgg
     6301 ccattactgg gataaattcc tctttgacaa atacgctgaa tattatcccg atacaaggct
     6361 ttttcacctg caacgttccg gatatgcagg aacccagcgg ttcgccgctt ttccctggac
     6421 gggcgatgta tcgcgaagct ggggaggctt ccgggcgcaa ctgccgctgt tgctcaccat
     6481 gagcctgagc gggctggcat atatacattc cgatgccggc ggttttgcgc agggtgtccg
     6541 cgacgaggaa ctatacaccc gctggctgca gtttgccgtt tttactccga tcctccgccc
     6601 gcacggttcc aatcatccca gcgaaccggt tttctggagt gaaaaaacgc aggatatcgt
     6661 ccgcaattac atgaaccttc gttatgctat gctgccttat aactatacgc tggcatggca
     6721 aaacacggtt accggggcgc cgttgatgag accgcttttt tatcagtatc cggacgatac
     6781 tactgccatg aagaccggca atgagtattt gtggggcgaa agcttcctgg ttgcccctgt
     6841 taccggtaaa gggcaaaaat cgatgaaggt atatttaccg cagggtccct ggtacgacct
     6901 gaaccgtgag gggaaatatc agggaaatca atggcttgac tatcctgtaa accttgaaca
     6961 gatgccggtg tttatcaggg gcggaagctt cattcctttc accgatacca tatcttcgac
     7021 tgattattac gacccttccc gttatacagt gtggtatttc ccggagggga aaacaaccta
     7081 tgagcaatat gaagatgacg gcagggataa tcatgctttg gcgaatggga actatgaaat
     7141 catcaggtac gaagggaacc gggaaccggg gaagattaca gtaaccatcg aacgtaaagg
     7201 ccaatggaaa ggaatgaccg ggaagaaaat cattacccta caggttcctg cgataaaagc
     7261 gccacagcag gttaccctga atggtaagac tttaaaatca ggcactggtc aaagcctcag
     7321 cgctttttcg tggagctatg acgggcattg gatttccgta aaattccccg tggggaaaaa
     7381 gccggttact gttcagatca acgattaatc gaaaactgtt aaaccaataa tattgtcatg
     7441 aaaaaggtcg tcctgctctt tattgtaccc ggattcatac tggccggttt tgggtcctgt
     7501 aaaaccaacc ctgccgaact gaaagccaat tatcctgaat gggctaaaaa tgcagttata
     7561 tacgaggtaa acatccgaca atatacgcca gaaggtactt tcaaggcttt tgaagcccat
     7621 ttgccaaggc tgaaagaact gggagccgat attctctggt ttatgcccat ccatccgatt
     7681 ggtgtaaaaa acaggaaaat gcccgaaggg atgaaggaaa gtcttggcag ctactattcg
     7741 gtccgcgatt ataaaggggt gaatccggaa ttcggaacgg aagaagattt caaaaacctg
     7801 gtggccaaag cccacgaaat gggattcaag gtcattatcg attgggttgc aaaccatacg
     7861 gcgtgggata atcagtgggt taccgggcat cccgagtggt atgagaaaga cagcaccggc
     7921 aagatacttt cgccattcgg ttgggccgat gtggccaagc tggattatcg caataaggac
     7981 atgcgtaagg aaatgatcgc atcgatgaag tactggattg aaaggtgcga catcgacggt
     8041 tttcgctgtg atgtggccgg agaagtgccg gttgatttct gggaacaggc acgcgccgaa
     8101 ctcgaaaaaa cgaagccttt gtttatgctc gccgaaaatg aagataagcc tcaattgctg
     8161 atgaaggctt ttgatgctaa ctatggatgg aggatgcatc agttaatgca cgacattgcc
     8221 aaagggaaag attcggtcca ctccattgtc aaacaggtgc ttaaagtaga ctcggtaata
     8281 ccaaaaaaca cttttcagat aaatttcatc accaaccacg atgaaaactc ctggaacgga
     8341 actgcacagg aaaagttcgg gcccggagaa aatgccttcg ccctgcttac ttataccctg
     8401 ccgggtatgc cgttgattta cagcggacag gaagccggtc tgaataaacg ccttgccttt
     8461 ttcgtgaaag ataccataaa ctgggaagga aatgacttat cgccgtttta tgaaaaactg
     8521 aatgccctga aacacggtaa tgaagcgctc tggaatgcac cttacggcgg caggttcgaa
     8581 ctggttaaaa acactgtccc cggcaaagtg ctgtcgttta tccgcaagaa aggaaagaat
     8641 aaagtgcttg ttatcgtgaa cctgagcaat aaatatgcaa cggttaacct caaaggcgac
     8701 aaagccgacg gcgattatac cgatgtgttc agcggagcgg cttttacgct gaacagttcc
     8761 accctgaatc ttacattgca gccctggggc tactgggtga tggaagcaaa gtgatctgat
     8821 ttatcttcat ggtcggcagg ccaatgtatc catcattgtt gcaatacaat cagtccgacg
     8881 gctcatttgg cattctcacg aaaacagaag attcctgtga gggaaagaga atttgatcga
     8941 ataaccgtgg tttgataacc ggctattgtt tgataacaat ttcgtgggtg atttccatgg
     9001 ctttgcggta catgtacgaa accccgcaat aacgttcctg tgaaaggctg atagctttct
     9061 gcagtttatc catcggcagg tccttaccgc cgaaaatata tactatgtgc atgtgtgtat
     9121 agtgcatagg atgttccgct gtaatatcgg caatcacatc cacgtcgaaa ctgtccagtt
     9181 ccacatgcat cttttttagg atggaaacca catccatgcc cgtgcatccg gccagcgcca
     9241 gcagcatcag ttttttcggg cgggggcctt catcgtttcc gccaagttca ggggaggaat
     9301 ctatggtgat tttatggcca tcaatctggg cttcaaattt catattgcct ttccattcgg
     9361 cactcagggt ttgttccatg atatcggttt ttgattaatg aagacaaata taatgatagg
     9421 agtaaacatc gcaggcatga aaaagttcag gcatctgaaa ggtttggcga cgggccggtg
     9481 attctgaaga agcaacagaa gaaggacgat gacgttcctg aaatatgttc ttcccgtcag
     9541 aaaaagtatt ctgctatccg gaggctgtcc ggaaactact caggttcttt tctttctgta
     9601 ataagtcttt tacaaacccg gcgacttccc cgtcagcatc tttttcaata tcgaatacat
     9661 aaacattccc gactttccag ttcgaagccg ccgcctgcac aaggttgctc cttacaaccc
     9721 agagctgggg ctttccggtt tctgccagtt cggtcacagc cttccaccat ccctgccggt
     9781 tcatttcaag cgggccgatc tcatcaataa tcacaagctg attcccgtcc agattttccg
     9841 gcgacagtat ttcgttccct gtgtccaatc cgttctgatg aaaaatgaac cttccgtact
     9901 ttatcccttc aatacctttc tcccggcaaa gaagcgtcct ttcacctgtc gcaatgttga
     9961 tcaggtcaaa accgacccgg ttgccgtttt cccggacagc gggagccagg aatcccgtca
    10021 ctgaaaatcc cgaacttttc aattcccggg caacgcgggt aacataagta gttttgcccc
    10081 catgaagttg tccgctgaca acgataattt caggccggtt cgagatctgc cgttcaaaca
    10141 gcgacaacaa atcctgcgaa taacagaaca gaccggaaaa tgctttaagc gggttcctga
    10201 aaattccgcg ggcgccgggc agcatcctga agatggccgg caatgatgag aacgccaggt
    10261 ttaccgattg atacaattca gccatgccct tgcgcatggt gacagctctg atgacgggat
    10321 ttttcagctc gacgctgatg gccgaaaaac ccgcaaggat aatagccgcc ctcaggttca
    10381 tgataagccc tgttttcagc ccttcgaaat tgaagtattc tccgccggca agtccgttga
    10441 ggagaaaagc ggcgataagt gtgatgagga caatttgcac ccagaagttc acctttttca
    10501 ggtgacgggt agaattccgg tacctcgtca ggacaaacac aaaatatgcc gttacagcaa
    10561 gcactgccag cccggctggg gccatattta ccaggtacag tgccatcggt actgccgcaa
    10621 tattaatcat taacagccac agcgaatagc catgttcatg cttcccgtca aacagggttg
    10681 actccgtttc gaggaacaac ctctttttct ccggacttac gccacaattt tccagactat
    10741 ggctagccat cagatatgcc gccagggctg ccagggcgcc ggtcagcaaa tagagaacaa
    10801 cgatcaggag gatcagctta ccgggttgca ggttcggaat ccctgcctgt cgcaccgcga
    10861 aaacatataa tgcctcaagg attttaatca ggttgagacc ataaacgatg agcagggtca
    10921 cagctttgtg aagcagagcg ctgaagaccg ccagcgcacc gccggtcaaa aaacctaaca
    10981 ggtttcttcc caataaacag atggagagtt caagcaacag ggcttcagcc atgataccag
    11041 tcatgggccc gaggatgatg gcgcttggtg aaatggattt cagcagggcg catatcgctc
    11101 ctgctctcca cacaaggcct ttctgcttcc agacggcgac aaacgacacc aacagaaata
    11161 ctccggccga agccagtatc gtccccgaaa gcggcacttt caggttatgg agaaaactgc
    11221 cggccacaat ttcgaatgct gcccagtaac tcccgataac ggaagcctta agccagcgat
    11281 cgtccggttt gtgtgccctg attatctcca tcctaagttc ccgatgaacg aaatatgatt
    11341 aattccaaca acaaaacagc cgtcgacgaa atgaatggtc attgaatcac tttgaacaga
    11401 gcagcagatt tgccccgccg gctcaaacag caggatacgc ccgggagcgg ctgtattaag
    11461 gtcgaagtgc atatttctcc ttttcaattc cagtttcaac ttccgaaaga caaagctgtt
    11521 taacgcctta tccgcggcaa agcactcaca ggctcctgac ccttagaata ctatgcccgg
    11581 agaatttatt acaatattaa aaagaaaaga ttcaaagaaa aagatttaaa caggtttcag
    11641 actgcacggc tggaagattt tttaaaaata cgggggagaa tatcaggaat gagattccgg
    11701 ccgattggaa ggaaaaatgt ttgtctgtaa tgacggataa agccccaatc aatgcagatg
    11761 ctacagaaag cggaatcagg agttttctat aaaaaaacca aaccgttaaa agcaatcgca
    11821 tggataaaaa atgtttgccg gataaataat agctggttat aaagcatcta atttaataca
    11881 ttattgcctt acagataaat aataatagca atccccggat ttgtaccttt catcctttag
    11941 aaattccgca gcttcttaaa gaataaacaa ctgtctccgt aactcaggaa acgaaagtta
    12001 tgttcaaggg cataatcata ggcttttttc cagtcgtcgc cgataaaggc ggaaaccagc
    12061 agcaacaggg tgcttttggg catgtgaaaa ttggtgacga tggcatcggg aatatggtat
    12121 tcatagcccg gtgcgataag caacgaggta tatcctttca gttctttcac ccggaaacgc
    12181 tgtaatgtac tgataacgca cgaaagcgat tcgttcaggg taacaccctg gtattcgtct
    12241 ttatacggat cccattgttc cacgagcatc gccggcgaac cggcttcgtg tttcagccat
    12301 tttacaccct gccagtacag gctctccatg gtgcgggccg tagtcgtccc gacaagagta
    12361 atgtgtctgc cggggttgtt cagcaacttt tcgatgatct ccaccgggat gatcacctgt
    12421 tcggcatgca tggcatgtcc gctgatcgta tcgctgctca caggcttaaa ggtgcctgcg
    12481 cccacgtgca gggtgatggt gctacactcg atttgttttg cagccaatgc tgccagcact
    12541 tcaggggtaa aatgcaatcc cgccgtgggg gcagccacgg agccgtcgtt ccgggcatat
    12601 acagtctggt acgtgagatt gtcgctgtcg atcacctcac ggtcgatata cgggggaagc
    12661 ggaattttgc cgaaagcttc gagcacgccg gcaaacgtta cttcggccgg cgaccaggaa
    12721 aaactcacca gaaaagaatc ctgcaaccgt tctccttttt cggccttcaa ttcaacttcc
    12781 gaaccatcga tggtagcttt taatgacaat atttcgcctt tccagcgttt ggcattcccc
    12841 accaggcatt tccaggtaca gcttcctttt tgttggaagg cgagctgcac gtcgttcacc
    12901 ggggaaaacg gttcgaggca gaatatttcg atacgggagc cggaaggttt ataaaacacc
    12961 agccgggcat ggataacgcg tgtttcgtta aagataacca ggcttccggc cggtaaatga
    13021 tccggtagtc ctgagaaaac gtccccggta atgattcctt ctttacaaac cagcaacctg
    13081 gagctgtcgc gctgcttcag cgggaactgc gcgatgcgct ccggcggcag gggataatca
    13141 taatcgctga tggctatatg gcgtggatcg tgcaaagcat ctgaatttga aatgcaaaag
    13201 taagcagata tcgtgaaact gttttgttgg gaatattcag aaaacccttg aaagatatct
    13261 ataaattgag gtaaaattga tatttttgct tttattacca gtttataaaa caaccggaaa
    13321 aattcttaga gcaatgaccg acaaatccaa agaactaatc acctctcttc aacttgtcaa
    13381 cgacaagctt cattttgaag gcaccgccga cggcaacccg cccgtctcca ttgattacac
    13441 gccgccgctg ggcgataacc tggggtacac ttccctggaa ctgctcctgc tgagccttac
    13501 ttcctgcatc ggatcggcga ttctcacctt cctgcggaaa atgaaaaaaa ccatcaacag
    13561 catcgaaatt aacggccggg gattccggaa ggaagaacac cccaccggct tcaaaaaaat
    13621 tgtagtggat atccggctca catctcctga tgtgacagag gaagatttgc gaaaagtcat
    13681 taaactggcc gaagatacct attgcccggt ttggtccatg ataaaaggga atacagaagt
    13741 agagactatt ttcaccatta aaaattaaca ggttattcag atgcaaattc aaacttaacc
    13801 catcctttat caaaaaatga aatcataggt attctcacta tttttagtgt tattagggat
    13861 tgcatcgtcc ggccagccgc aacagcaact caaaacaggt aatccttaca tgaatgtcca
    13921 gttcggaatg gctcgcctgc tgaactgcga tgaaatctca aacccgggga agtttaaatt
    13981 ctcaaagaca atctccaccg gcatcacaat cgaatacagg attccggtca gcaagacggt
    14041 gaatgttgaa gggcaaggtt ttacggccgt taccggcatc ggggcgaacc tcctgtcgta
    14101 cagcatcgat aaattgctcc gcgacagcac cgacatcgtg atcagcgcga taaatgccgg
    14161 ttatgattat aatgcgcttc agggattcta tttcgatatc ccgcttggaa tctgttatat
    14221 aacgccatat aatgacaggc atcataattt ctcgtttgag ctgacaagcc ggaccggctt
    14281 tttagtctgc agccggagct atatcaaaat ggataccggg ggtgaaaaga aagaaattgt
    14341 agagaaaaat acgggtctgt taaacaggtt ccacacgggg ttcaatgcca aagtttcctg
    14401 gaatgtaatg tatttggaac cgaaaaagaa tgaactgaca ggttttagat tttcattgtg
    14461 gggagaatac tatccgacca atgtgtttcc gtccggtaaa ggtgtttcag cccaaacatc
    14521 cgccgcaggg gttggcatcg gctttaattt ttgacccgga taatgtcctt tacaggatgg
    14581 cgcgttatcc cgatcctgtt tctgagagaa tttcgacggg tataaacata cccaatcgtc
    14641 agggtatcgc gttaaatatc atctgccgat ttcattcgaa atatccccga tactgatctg
    14701 gttttcatac agggtcagca cgtcggcgga atccacaacc atgatcctgt cgatgcggtt
    14761 ggtaacctgg tgataccaca cttcgacgaa aaggcctttc agggtataga gcctgatatt
    14821 gaaaaaaagg tagaaccttc ccgtcagttc atggccttca tcaaaaagga gctggatttg
    14881 ttccatccgc ggaagctgcc tgaaaatgct ggaagtcata atacggaact gtttaatttc
    14941 ctgactacaa tcgctctatc tcatgggcgt accttaattt caggtagcta agcttttcaa
    15001 ggtcgccttt caggtcaaaa atcctcacca tttcaatcat gccggatttg tcatagatgg
    15061 atacctgggc ggcatcgtaa attttatgcg tccagttgtt tcggatgtgg attgtgatcc
    15121 tttcaaaatc ttcccattcc agaaaccccg gaatgcggaa gtagtagatg tcgtcctggt
    15181 ccattgattt atagatgcct tcggccatcg ggctcatcag gaaaaacttc ctgattttga
    15241 tgaggctcat gtaggccggt acagtgcgat gcggctggaa ttgcagtcct gcctgtttgt
    15301 aaaagctgag caattctcca acccgggaat agtcgtccat aaaaaccctg atacagggct
    15361 ccggggtatt aaaaagctgc agtactccca gggctgcatc aaaatccggc ttatacctgt
    15421 ttttgatttt cgtcgtcatg cggatgaact ggtcttcgcg gcaaacatca aactctttta
    15481 agatcaggaa tacggaacgt ggcctctttt ccctttcact ggcaggaata aaatactggt
    15541 cgtaaaatcc cgggtactga tcaaggtctt caaggatcag atccttaaaa tttatatccg
    15601 aggccaggct ggctagtttc tcctttttga gaatataccc cagcgattcg acaggcttaa
    15661 gctgatccat gacctgatag gtttaccggg tggataaaag gtcgacttta aacaaagtta
    15721 aggcttttta tgatcaaata caaatattta aacaatttac acggcataaa agtctaatga
    15781 atatcataag atatgaaaat cgtatcttta ccgggtacta actgtatctg accatgagta
    15841 tccgcaagca tatccccaac tcgttgacaa gcctgaacct tttttcgggt tgccttggca
    15901 tagtggcggt ttttgaggac aacctgccat tggccgcctt gttgatggct gttgccattg
    15961 tttttgatta cgccgacggc atggcagccc ggctgctcca tgcaaaatcc gatatcggtt
    16021 tacaactgga ctcgctttcc gatgtggttt cgtttggcgt gttgcccggt tttattgtta
    16081 tgaaaatgat ggaaaaatgc agttcccttc ccgattgcgc tgtttccggg ataaatttat
    16141 gccctttcat cgctttgata atcccggttt tctcagccct caggctggca aaattcaaca
    16201 tcgacacccg ccagacagat tcttttctgg gccttcctac accggccaac gcaatattct
    16261 ttggttcgct gccactcgta attcagcaat gcaatgataa tgcctggaca ggacttgccg
    16321 gctggctttc aaattggggg atacttaccg ggctgacctt gatattctcc ggtttactgg
    16381 tatccgaaat tcctttaatg tcgtttaagt tcaaaaacct gtcctggcaa agcaataaat
    16441 tccgttttat cttcctggct attgcccttc tggtactggt attcctgaaa tttgcgggta
    16501 ttcctgttgc cattctattg tatataatgg tttccctcat tacagggaag caaaaaagcg
    16561 acaatgggca tgtttctccg gtcatcaaag ccggagggaa gaactaaata aaggtttctt
    16621 gctgatttac tacctcaact cgaaattttg tccgaggtag accttgcgta cttcttcatc
    16681 ttcggccagg tctttcgagg tccccgattt cagcaccgat ccttcaaaaa gcaggtaggc
    16741 gcggtcggta atcgataggg tttcgtgaac gttatgatcg gttatcagaa taccgatatt
    16801 tttggttttg aggttggata caatatgttg aatatcctca acggcaatgg gatcgattcc
    16861 ggcaaaaggc tcgtcgagca ggatgaactt cggatcggtt gccagtgcgc gggctatttc
    16921 ggtacgccgg cgttcacctc ccgataactg aattcccttg ctcttacgta cattctgaag
    16981 cccaaattcc acgatcagtt tctcaagttt ttcccgctgg gcctgctttg acagaccggt
    17041 aaattcaagg acggccctga tgttatcctc aacgctgagg gtgcggaata ccgatgcttc
    17101 ctgcgccagg tacccgattc ccaactgggc gcggcggtac ataggttcgt cggtaatatc
    17161 gcgcttgtcc agatacacgc gtcccgacaa cggcttgata agtcccacaa taatgtagaa
    17221 tgaggtcgtt ttcccggccc cgtttggtcc caggaggcca acgatctctc cttgctccac
    17281 gtgtacggta acattattga ccaccgtgcg ttggcggtat ttcttgacaa tattatcggt
    17341 acgaagcagc ataatcgggt gagagaataa gcagcaaaat taagtaaaga gtttcttata
    17401 tcagaaaata aagttcatgg aaaccataac cccgaaccgg tttgtatccg gatgatcgtt
    17461 gtaagtcaat cgccagatgc cgtcaatcct gataaaacgg aaaatattct caatgcctgc
    17521 ccccacttcc acatagggct tgcccaatgc gtaggtgccc gttgggaacg aattgtactg
    17581 gagattcttg tcactggtgt gcccaaaggc aatccgggcc tgggctacct cacgccattt
    17641 tagctttctg atgacaggga tatggttgag gaaaaagcct tcaaaatgat gtgaatacat
    17701 ggcgttcacg tattcatcgc tgatgaattc ataataattc atcaggttat acgagtattc
    17761 atcaaaccag aatgtttcgt tcccggaatg aaccctcagc aacggatagg ggagtttgcc
    17821 ccagattttt ccggcttcaa cagaatattt agaccagcct gccgagagga agttgaacca
    17881 ctgggtaacc ctgagctgcg ccttgtgata ttcatagtcg ccattaaaga gccccttgac
    17941 cccgtatgta taatttaaat caagcaccgg atatggtgag cttaccacaa cacgttcaaa
    18001 ttcgcctgcc agtacttttt cgcggtaacc gtaatgaaga tcaaactgaa gttcagaagt
    18061 aatcaggctg aaatggggtt cataactgcc tccctgcgga tcgtaaaggc tgaatcccgt
    18121 gttaccgata ttgtacaggt tacggttgga aaaggtgagg gtatttgaaa atccggtaaa
    18181 ccattcatgt ttgtatgatc ctttgatttc ctttaccagc gataatttat cctggggatt
    18241 gcgccggaaa acagaactga ggaagaaatc ttcacgcagg gcgtcttcac ccatgccaag
    18301 ctgctccgta tcatatttgt atgagccgga aatcaccctg tcgggagctt tctggagcat
    18361 ataaacaaat cctcctttgt atttcagacg ttcatcatga atcccgtagg ccatataacc
    18421 ttcaagcatc aggcgcttgc tgaaatcgtt gctggtgcgt cccccgaccc ttagccggac
    18481 tccttctatt gcgttggaac ttagcatgga tgcgtaagga ccccattcaa atttgccttt
    18541 cacataatag ccggtcatca ccatcttgat gacatcgaaa taggtgttga acacgggcat
    18601 tttcagcagg gtgtcggcca gcaggtatac cgatgcttcg cggtgtgtca gctcttccgg
    18661 gcgcgattct tcccagaaat ttttactttg cacggcggca tccttcagga cgataatgtc
    18721 attgggaatt gaatagattt tttggtccgg tggagcatca aaactgaaat tgcggaatac
    18781 tgccgtccgg gcgccataaa accccattgt tttctttgca tcttcaaaca cgttgaagtc
    18841 ggcaaccatc cgttcctttg atagcatcca gtgtttgccg cccattttgt caaagtcctg
    18901 tgaaattaca agatcattga tgaaattaac attggcatcg tcaaccaggc gcatttccag
    18961 tttcttaacg gcgaacgatg tgtcgttggc ccacaggtta ccggtaaacg ctgtagcctg
    19021 tttaaagcgg ggcttaaaca tgatgttgta gcaccagtgt ccgtctattg ttgcggagtc
    19081 aatcaggtaa taccggtagt aaagcaggcc agtcgaattg atcgggcttg gaaaattttt
    19141 ctggaacaag gtgatgaaat tctcatagat gttcacatcc tgggccagtg aaccaacaaa
    19201 ctgcgacatg ctggcatttt cgatgccgga gatctttgat gctttgataa tttctttccg
    19261 gctgcggggc gaataacggt aatacacatc cgataccgat tccgataaca tgacaggcag
    19321 gtatgctttg ccgttgacca cgcttgtatc aagattttca aaaacgaacc ggaaaggttt
    19381 catgacgcgc ctgtcctgaa atttggagga aaggttgttc acgtcaaact gcattttggt
    19441 ataggtctcg caccggtact catcaaccct ggcaggatca ttttctggtt tatggtcgat
    19501 gatttttttc agcagaattt cggccgggtt tactcccgga acgatcacca cttcaggaag
    19561 atcaaggtta acaggattca gttttatgtc aattacctgg tactggtttg gttttaccgc
    19621 tgacgaaaag gtgtgatatc ccacaaagga ggcaatgagt gagtcggcaa ttttacgcga
    19681 ctccagcgag tattttccgt cgaaacctgt ggtggcgcca acggttgatc ctttgaaata
    19741 aacatttacg aagggcatgg gctgcccggt tgaagcgtcg gttactgttc ccgtaatctt
    19801 agtaacttcc tgcgcatgca acagcaaacc cgacatggca aaagtcagta aaaatgacag
    19861 ttgcagccgt gatggcaagc attcgccgag atacttcatt ttcataggag cagcaataat
    19921 caaacatcat gccttaccgg ggaatgtcat cccaaaattc aacagccctg cgcaaatggg
    19981 gaatcacgat ggttcccccg acaagtgttc cgataccgag tacttcaaat aattcttctt
    20041 ccgtaacgcc gttttcgcgg cacttcagca aatggtacct gacgcaatcg tcgcaacgga
    20101 gcacgagtga agttgccagc ccaagcattt cctttgtttt tacatcaagg gctccgggag
    20161 cgtaagcgtt tgtatcggca ttaaagatac gtttaataac cttgtcgtcg gcagacagga
    20221 tgcgttcgtt catccgttga cggtacgcat taaattcatc ggcttttgtt cccattgtaa
    20281 tagtttttca ggtaaaaaca gccaaatgat ctgacggaag ggcgctgtca gataacccct
    20341 tctttcagga tatcatgaag gtggataaca cccgaatagg tgccgttgtt catcacgagg
    20401 agctgggtga tattgtttgc gcgcattaca ccaagggcat cggaaaccag ggcatcgcat
    20461 gaaatggttt tggggttcac cgacatcaca tcccgggcag tgaggttaac aaatgaatcg
    20521 tgcgtatgaa gcatccgccg caggtcaccg tcggtaatga cgccggcaag tttgttaccg
    20581 tcaataactg ccgctacgcc aagcctcttc gatgatattt cgagaattac cgttttgata
    20641 tcatcgtcag gttttacttc aggcttttca ttgaaaatgt aaacatcgga gacgcggagg
    20701 tagagctttt tacctaatgc cccgccggga tggtacctgg caaaatcgcc ggctgtaaaa
    20761 ccgcggcatt cgagcagcgc tgtagccagg gcgtcgccca taacgagttg ggcggttgta
    20821 ctgctggtag gggcaaggtt attgggacaa gcttcgcgtt caacggtggt atcgataatg
    20881 taatcggctt cccgtgccag gtatgagccg cggttgccca ccatggcaat gagcacattc
    20941 cccatgagct tgaggagagg ggcaagcact ttgatttcgg gcgtattgcc gcttttagag
    21001 aggcatacaa caatatcatc cttgccgatc atgccaaggt cgccatgaac ggcgtcggca
    21061 gcatgcatga agatagcctg ggtgccggtt gaattgagcg ttgaaacaag ttttgccgcg
    21121 atattggcgc ttttaccgat gccggtaact ataaccctgc ctggtgaatt gaaaatgagc
    21181 tgaacgcatt tcacaaaatc gtcgtctaca agacgggcca ggttttcaac tgcctggctt
    21241 tcctccctga tagtatctaa cgctattttc ctgatctctt ctgctgtttt cactacgatg
    21301 attgttgaac ctggcatcaa aggtaaaaat aaaatcacga atttttctga aatcatagat
    21361 accagcagtc acaagagttt tcgcatgaat ttaccctgct gctgtaaatc aatcatatgc
    21421 agtttttggt gttcggagaa aataaaaata tgcagtttgg atatttcatc ctacttttga
    21481 aaactttttg gcaaaaggaa aagggttcat aaaaatgttt tctaatttga gccgttgata
    21541 tccaacattt tattgtggtt ccggcaagga attgggcaat aaatcgggaa aataaaatta
    21601 attccggcgt cgaagaggtg tcataatttc ttaactttaa cttaagaaac cggattgaaa
    21661 gagaaaagct tctgcttttt ccggagcagc ggctttttta agttaatggc tttaacctgc
    21721 aaaatgaatc cggatcggtt ccggaaatga ttttagatag cataaaagtt aagaaggttg
    21781 aaaaatgatt ttgttaggac tacttttgca ttcatactaa cctgcaaaaa ccggaaaggt
    21841 attaatacaa ggcagcctga taacggataa gcaagcaatt gctaataata ctgaaaatga
    21901 gagtaaagaa agttactgaa gactacaccg actatccgct ccacgatcta ctgaagaaaa
    21961 ttttcggatt tgagagtttc aaaggaaacc aggaggctat catccgtaat gtactggccg
    22021 gcaacgacac ttttgtcatc atgccgacag gaggaggcaa gtcgatgtgt taccaactac
    22081 cggccatcat tatgccgggt actgccatta taatttcacc gcttatttcg ttgatgaaga
    22141 accaggttga cgccatccgt cagtttggca ccgaccgggg tattgctcat ttcctgaatt
    22201 cttcgctctc gaaaaacgaa attgccgagg tgaaagaaga tttgctgaga ggcaatacca
    22261 aactgctcta tgtagccccc gaatctctca caaaggaaga gaacatcgct ttctttaaag
    22321 atatcgtcat ttcgttttac gccatcgacg aagcacactg catttcggaa tggggccatg
    22381 atttcaggcc tgaataccgc cgccttcgtc ccattatcga aagcatcgga caggatgttc
    22441 ccgtaatggc gctcacagca acggctacgc ccaaggtaca gcaggatatc ctcaaaaacc
    22501 tcgggatgca ggaagccgcg ctctataaat tgagcttcaa ccgcccgaac ctgtattatg
    22561 aagtaaggca aaagaacgaa gacgtcaaca aggaggtcat caagttcatc aagatgaacc
    22621 agggcaaatc aggtatcatc tattgcctga gcaggagaca ggttgaggaa ctctccgaaa
    22681 cgttgcaggt taacggtatt aaagcattgc cttatcatgc cgggcttgat tcgcccaccc
    22741 gtgtcaccaa ccaggataaa ttcctgatgg aagaagtgga cgtgatcgtc gccaccattg
    22801 ccttcgggat gggcatcgac aagcccgatg tacgtttcgt gattcattac gacatgccga
    22861 agagcctgga aggttattac caggaaacgg gacgctcggg ccgtgacgac ggggaaggca
    22921 tttgccttgc tttctatagt tacgacgaca tcgaaaagct tgaaaaattc agcaaaaaca
    22981 aaggcgttgc cgagcaggaa atcgccaatc aattgctgat ggaagttgta tcgtatgccg
    23041 aatcgtcgat gtgccgccgc aagcaattat tacattactt cggtgaagta tatcctgaag
    23101 aaaactgtgg caactgtgac aactgcctgc atccaaagac gaaaatagat ggccgcgagc
    23161 aggttgagct ggccctggaa accatactgg ctaccaaaca gcaaaccaaa gccaaacaca
    23221 ttgttaatat cctgatcggg aaggtaacct cccagatcaa acaatacaag cacaacgagc
    23281 tggaattttt cggaaaaggc atggaaggaa gcgagaggtt ctgggacggt gtgcttcgtc
    23341 agatgctgat tgaaaggctg gtttccaaag acattgaaaa ctatggtatc ctgaaattaa
    23401 ctcccgaagg gcaaaaattt ctgaaaaagc cttattccat actggtagcg caggattcca
    23461 atcctgaaga agaaggggaa gatgaagaac tgatggctgc cggcggggcg cggaccagca
    23521 ctaccgataa gacccttttc tcgcttctca aagatcttcg caaggaaata gcccggaagg
    23581 aaaacctgcc gcctttcgtc atctttcagg atccgtcgct tgaggatatg gccattcagt
    23641 atcccatcaa catggacgag ttgcagcgca ttacaggagt cggcgccggc aaagcccaga
    23701 agtacggcaa gcctttcctc gaacttattg ccaattatgt ggaggaaaat gaaatcatcc
    23761 gtccgatgga catggtggta aaatcggtag tgaacaaatc aggccttaaa gtatatatca
    23821 tccagaatat cgaccggaag atttcgctgg aagatattgc cattgccaaa aacctgactt
    23881 ccgatgaact gatctccgaa attgaaagca tcgtggcatc gggcaccaag atagacctga
    23941 attattatct caacgaaaac atagatccct atcatcagga agaaatcctg gattattttc
    24001 acgaagccga aactgattcg atacagcatg ccctcgaaga acttggcgag aatgagtatt
    24061 cggaagaaga gataaggctt gtgagaatta agtttatgtc ggaagtcggt aattaaactt
    24121 ctcttaattt tattactttc gcaacctgat cagcgataga tcaggttgtt gtattttatt
    24181 aattaacgtc aaatcaatcc ctatgactga aaatattact tccgaagaca ctcataatga
    24241 aatcgaaaac cccgttgaac ctataactga accatcgcaa cccgaagctg aacgacagcc
    24301 tgggaaacat ccccggaaaa ccgataaatt acagcttctc ctggctgtca acatattgtt
    24361 tttccttgca ttggcagtac tatatgtgct tttcttcagc aaccgtcagg gcggtaattc
    24421 ggaaagtatg gccaaaaccc tccaaaaaac cggcaaagga aatatgtctg tcgcttttgt
    24481 gaataacgac agcatacttg ctcactacga cctggtaaca aagctttcgg atgaactggc
    24541 cgggaagaca aaacggctgg aaggagaaat tgccgccaaa caaaaagcat ttgaaaaaga
    24601 cgcatcctat ttccaggatc aggttcaaaa aaaggcctta tccgatcaaa gcgcccagga
    24661 aatttacggt cagttacaac aaaatcagca aggcatcctg gaacttcgcg accgttatgc
    24721 agcagaattg cagcaaaacc aattagacat gaatgttgtt gtactcgact cggtaatgaa
    24781 cttcctcaaa cgctataacg aaaaatacaa attcgactat atactgggct ttaccaaagg
    24841 cggaaacatc ctgtatgcca acgacactct cgatataaca aatgatgtca tcaaagaact
    24901 gaatgaacag taccacaaaa agaatcctgc aaagtaatag tttgacagcg tgtatcagag
    24961 aattgtaaaa attatactgg tattcttacc ggtcatcatg ttttcgcagg cgtgtgacaa
    25021 ggtggaaggg gtgttttctt cgcgtccttc aggggtttta tcgaaaaagg agatgaccag
    25081 catgttggtg gatattcatc tgcaggaagc tgttatccgc accggcgtgc ataccggcac
    25141 tgataccgat atgcgaaatt attcccgttc aggatacctg aaagtgtttg ccaaacacga
    25201 tgttactcct gaagaattca aaaagagcct tgactattat ctcttacatg ttgatgaact
    25261 ggatgatatc tataccgggg tgatcaacca gttgacgcag atgcaggctg aagcccaggg
    25321 agagaagggg gcgcctgcgg cgaaaagccc ggctcccgga gccgatactg ccgggcacaa
    25381 cgtgaaaaaa gttcccgata tgcctgccgg acattaaacc ggcaaggaga tccaggcgct
    25441 aagttttaca gcttggttgc tacagcggta tattcctcaa tgtctccgcc cgcaatcagg
    25501 gaataggtcc agttcatagt tccggaactg accagttttc ccgatccttc aataacccag
    25561 ccattcgaca gcgtttgttc cgatactact atctgactgc tggtgacaat acccgaagac
    25621 tgaatatcga atgacccggg gtttcccagg ttctccagaa tgacctggct gctgttgtcc
    25681 ggatctttcg ttatattgac ggtataactg atcgacctgg tacttttcac cgagttttca
    25741 ctaaactgcc aggtgccggt catcttatcg cgggggtcgt cactgcccgg ctcgtcttca
    25801 gctatctcgc aggatgtaat ggccatcatg actaacaaga ggagaataaa gaggtttctg
    25861 gtgtaagttt tcatcttatt caaggtttcg ggttaaaaaa ttagttctca caggggattt
    25921 cggcgtattc tccgggaaaa attacctgca accggttctt ccgacaagta acggtgagga
    25981 accaactatc gtgccatggc tcctgatgat tacagtataa tatcccggaa gcacaccggt
    26041 aagattcagt atgtacggat ctgacggact gcgtaaaaat tcctcctgcc tgaccgctga
    26101 acccataaca gaaacgaggc tcagggtcag gtccccgctg agggaagttt gtattgagac
    26161 gctcccggtc gcaggattcg gaaatacttt taaaaccgga tcattggttt ccggcgcttc
    26221 ctgaattccc gtattgcatg gcattgaaat gcccccgcag gaagttatta cgctgtcgag
    26281 atgctgttgc gagggcggcc agatataatt ttcggcaata gcgcctcctg gtttgatgag
    26341 tattactgta ggatacgaca ataattggta atcgttcacc acatggttcc cgtttccttc
    26401 gagaccgctg gcggcaggat aataagcccc gtaggcatca cagaattccg tgacctggcg
    26461 gttattggcg ccccagttga tcccgagaaa aatcacattg cccgtgttac agccaaagta
    26521 ttcataggca gcattgattt caggagcata ggttacacaa ggcccgcagg ttaccgtaaa
    26581 aaagtcagcg acaaccagtt tatcctgctc aagataatta ttgaggtgat gggtgatccc
    26641 tttcacatcc ttgacggtaa aattaactgc cgtatcaaga ggcgtctggg cgcttagaag
    26701 gccggaaaag aataccagga atacgggcgt cagtaatttt ctgatcaatg ttacggtttt
    26761 tttaaagcgt tggggattgt ggcctttttt cagcagaagc cttttcttcc ggcagtttgt
    26821 cagattaacc gatcaatggt ttttccaggc ttccagttcg gcaaaaatag catcggccag
    26881 tcggctggcg ccaatcctga aaagattttt attcagccag gcatcaccta atttatcctg
    26941 caccactgca taataagcca gcgcctggtc aggagtggag ataccacccg caggtttgag
    27001 tcctacctgc ttcccggtgg ctgaaaaatg ttccctgatt acatccgaca taacggcgag
    27061 cgcttcgggg gttgcagccg gttgagtttt tccggtgctg gtcttgatga aatcggctcc
    27121 gccttcaatg gctagttcgc cggccaggcg aatattttcg gcagtcgcca actctcctgt
    27181 ttcaaggatc actttaaggt gagctttgcc gcatgctttc ttcattgcgg ccacttcgct
    27241 cctgatggtt gagaaatccc cggccaggaa tgtgccccgc gagagtacca tatcaatctc
    27301 atcagcgcct tcggatacgg catacccaac ctcagccact ttaaggtgaa tgggcaattg
    27361 gcccgaaggg aatgccccgg ctaccgatgc tgtcaatata ccgctgcctt tcagcctgtc
    27421 ggtcacaagc cgtacaaacg gcgaatagac gcatactgcc gcaggcgaag gggttccttt
    27481 gtctgaaaaa ctaagcgcct tgtcgcacaa agcattcacc cgtgacttgt tatcagtgcc
    27541 ttccagtgtc gtaagatcaa gaaaaccaag aatttgagtc aaagcgtcaa tgcgggattt
    27601 gcctttacta aaaggctcgg ttatagcttt taaatactga tccatagtag tgaaatttat
    27661 aatttttagt atgatgtgct gaacatcctt tttactacaa aagtattgat ttaatgtata
    27721 ctggaaatat gaatcactga acatcctaat aatcagtcaa taaaataaca tttcaggttc
    27781 aattaattat cggcatcaaa aaacagtgac aaagcgggaa tcaataattt tttttgcgta
    27841 agatattacc tatcaaacta ccagtgatta atagataaca aaatgccaga attcaccagc
    27901 ctgatcagct tagtgagact gacaatgaga ataaatcgcc ggaattatga gatcaatttt
    27961 acatttttta tcccaagcag ataacaggag cggctcaggt ccgtctcctt cgtgtataac
    28021 ctggctttca aggagttgga caattgtatt tgccagcagg gcagcacttc agcagcagtt
    28081 tgtaaaattc ctgctgatag tggttctcct gattccttcc ctgaaaatga atgccgaggg
    28141 cacgcgacaa ctggagcctt caaccggccc tgcgacccag tattgtaaaa tgtattttga
    28201 caacgatctt ggtcagttcc gaattccttt tgcgcagcta ggatgccctt ccgattaccg
    28261 tctttatttt tacatagatt accctgccac cgaggtggtt tattttggtt ttggttcagc
    28321 cattgattac gggaatatag cgcataacga tctgcagttc cagatcaaag atccttccga
    28381 caatgtagta tcaggtttca ctatggctcc gttgcctgta gccggcgcgg gttttatctc
    28441 cacgaatgcg caggcacagg ccggcccgaa tatttcaggt tccgtacctg ccggatatac
    28501 cccgctttcg tttactccca ccatgtccgg caattattac atcgaatttt cgatgaacaa
    28561 cctgccggat gatatcaatg gccgcggact gcgttatttc gaccttacgg tagccaacgg
    28621 cacaacgcct attgatggcc gcctttggtg caaggcgtgg caactaagtt cctcaacaac
    28681 ctctgccggc gcaacaggta aaacatttgc ttccttttat tcctacacgg ccgatagtat
    28741 tgtcacgcgt tttcaatgca acggacttgc cggcggaatt ttcagcgttt attgtaacca
    28801 atatgggaca aacaatacgg gtaactgggc cacagaccgc agatcgatca acggtaacgg
    28861 aaccgtgatt ccggaataca ggctgtttct gaacaatccc gataccaata tttatccttc
    28921 aggacatctg gggcgcattt gcgatgtttc ctctcatcct gaatgcgacg gttcggtcga
    28981 tatcctgatc aaagttaaca ggcccggaac tattttcgtt gacattgatt tattgcctgc
    29041 cggtgccggg cctgaagata ttacgttaat aaaagctgtt acaggcagcg ccaattgcga
    29101 tacctgggat acaataccct ggaacggaac gaacggcctg ggagtcgccg tgcagaacgg
    29161 cgtaattaac gatatgacga tcgtgtacct caacggcctt accaacctgc cgttatatga
    29221 tgttgaagat aaccgtttcg gcattaaagt agatctgatc cgtccaacgg tcacaggaat
    29281 acataaaatg cccatttatt gggatgatgt taatgtaggc gggtcaacca accttaccgg
    29341 atgcacttac cctgccggcg gaatagtaac cggatgccac ccctggacaa acacaaatat
    29401 gttgacccat aattcctggt ggtactatct taccgacggg cctgaaaatc ttacggttgc
    29461 tgtgtaccgt actcctcctg ctgctccgtc gccaacaggc ccctcacagg tttgccaggg
    29521 ccagaccagt gttaccttca gtattcctgc acaacaatca acggatctgt atatctggaa
    29581 tcttcccgac ggctcagtag atacaacgct tgttccttcg gtgaccttcg atattgattc
    29641 aacggcagcc gcctccggaa ccatttcggt gatactgaat aattccaact gcggcaatgg
    29701 ggctgcatcg gcgcctttca gcataagtgt gactcaaacc ccgaatccat ctccgaatct
    29761 catcgtaagg ccctgcccga ataaaacagg cgacctgaca acggttcagc cgttggcggc
    29821 agcaggaaca tccctgcaat ggcatacttc ggccacaagt ccttccggtg caaccttggt
    29881 aagcactcct tcagcagttc cgtcgggtac ttactacctg tttgctgtaa atagtactac
    29941 ggggtgtttc agtccggctt caccggctgt tgtggtgagc tgcgacacct gtccgcccgt
    30001 tgccgtaacc gattcacttt ttacctcgaa tacgctggcg gttacagtga atatcttaac
    30061 aaacgatttc gccggtgatt atgcaattaa tccggctacc gttgaccttg accctgcaac
    30121 ccccggtttc cagcaaacca taaccattcc cgggcaaggc acttttacag taaactcaat
    30181 tggcgtgctg actttcactc ctgtgagttc ctttgtcggg atcgcaacgg tgccttatac
    30241 tataaacgac atttatgcca acacctccaa ccaggcatcg gtaattgtaa ctgtcggtcc
    30301 tgacggagtt tcagtaccgg cttaccagat catctgctcg ggtgatacgg caatcatcca
    30361 tctttcgtcc agtattcccg gagcaacttt cacctggaca gtaagcgggc cagcttcagt
    30421 tacaggacaa actcccggtt cgggaaatct gatctcgcaa attctggtca acaacagcat
    30481 gtcgaaccat acggttacgt attttgttac accctggtat ggaggtattg ccggaccggt
    30541 tgttactgct caggttgaag ttacaccgcc ggtaaccctt accaacgcca cgctgttaaa
    30601 aactatctgc tcaggcgata atacgaatat caatctcaat acttcgccca acaatgccac
    30661 ggtaagctgg gttgcttcca ataccggcaa catcagcgga attgtacctg cttcgggaac
    30721 tacaaaggca ataaaccaga cgctcattaa caatgatcct tttgccaatt atgtctacta
    30781 caatattgaa gtttcctatt atgggtgtgt gacggatccg gaaacctata cggtgaccgt
    30841 taaccaggat cccgatgtac aggtttcccc ggcttccagc gtaatttgtt caggaacctc
    30901 cgtcaatctg gttcttacct caaccatacc gggaacaacc ttctcctgga cggcttcagg
    30961 aacagccggg gtaagcggtt attcggcagg taatggaaat acaatttctc aggctgtttc
    31021 cactgccgcc tctgttcccg gcacagtaac ctataccata accccgactg ccaacggatg
    31081 tgccggtacg cctgtcaact accaggtcac ggttaatcct gttccttcag tctcaaatcc
    31141 ttcacttgcg gaaactattt gctcaggttc atccacagcc atttctataa catccaatat
    31201 tgctgctgca acttttacct ggacagcttc cggctcagca ggtataacag gatattccgc
    31261 tggtgtcggg aacatcattg cacagacact tgataacccg ggcaacatac ccggaacagt
    31321 tacctattcg ataactccca gcgcatacgg gtgtaccgga agtaccgttg attatattgt
    31381 gaccgtcaac cctgttgtga atgtcacaac aagcccctta aaccagtctg tctgcaccgg
    31441 atacagcacc ctgattccgc ttacgtcgaa tgttgcagga gccacatttt catggactgc
    31501 ttccggaact ccgggtatca gcggttattc agcaggcagc ggaaatgtta tcagccaggt
    31561 gcttaccaat tcaattgcca ctcccggaac ggtaacttat acgattaccc ctgccgccaa
    31621 cggatgcaca ggcgttagtt ccagtaattt cattgtcact gttaatcccg aaccggttat
    31681 tttcaccatg gttcccaccg gcccacagtg ttccggcaca atattaaggc ttaacggttc
    31741 ggaaagcgga ctgctttaca tacttcgcct taatggtata agtgtcgata ctattacagg
    31801 aaccgggctg gttggtttcc tggatttcgg cccccggtac gatgcaggta tttatgatat
    31861 tattgccgtc aaccctgtaa ccggatgcca ggccctcatg aatggcttgg ttcagatgaa
    31921 ccccgctccg gatatctttt ccgtacttcc agccggaatt ctttgccccg gcgccaatat
    31981 tggcctgagc ggatcggaaa ccggcatcag ctaccagttg cgctggaacc acatgatcaa
    32041 tatcggttca cccgtcagtg gaaccggaag tcccatcagt ttcggcaccc agatatttcc
    32101 gggcacctat acaatcatag cagttaatcc ggcaacagga tgttattccg aaatggccga
    32161 cagtgccaca atctatcctc aacctactat ttatactctc acggctaacg gtacaggatg
    32221 tccgggtact tccatcggtc tgagcagctc gcaactcctc atcgattacg tattgttatt
    32281 aaacggaacc attcacctgg atacagtttc cggaaccgga aacccaattg atttcggccc
    32341 gcagacgact gccggcagtt acaccatcct tgctatcagc caaagcaact tctgtcagat
    32401 attgatgaac ggaacctacg tcatcaatcc tgatcccctg gtatataacc tgacgcctgc
    32461 cggttatatt tgtgccggac agtccattgg tttatccgga tcggaaacag gaatttcgta
    32521 ccagctttat aatggggcta ctttagtcgg atcgcctgta gccggaaccg gaagcgccct
    32581 tgatttcggg cctcagacca ctgccggcgt ttacaccgtt attgccacca atccggcaac
    32641 gggatgctat ttgcagatgg ccaacagcgt cacggttaat cccaatcctg taatttattc
    32701 gctcacttca ttgggcgccg gttgcccggg taccactgtc ggactgaacg gctctgagct
    32761 taacaccaac tatattctgg ttctcgatgg tacgctgtac ctcgatacaa tttccggaac
    32821 cggaaacccg gttgactttg gcccccaaac gactgccggg acctatacca tactggctgt
    32881 gaaccaaaac agtttttgcc agtcgctgat gaacggttcg ctcgtcatga atccgaatcc
    32941 gtcggtattc agcctgacgc ctgccggtta tatttgtgca ggccaatcca tcggcttatc
    33001 cggatcggaa acaggaattt cgtaccagct ttataatgga actactttag tcggatcgcc
    33061 tgtagccgga accggaagcg cccttgattt cgggcctcag accactgccg gcgtttacac
    33121 cgttattgcc accaatccgg caacgggatg ctatttgcag atggccaaca gcgtcacggt
    33181 taatcccaat cctgtaattt attcgctcac ttcattgggc gccggttgcc cgggtaccac
    33241 tgtcggactg aacggctctg agcttaacac caactatatt ctggttctcg atggtacgct
    33301 gtacctcgat acaatttccg gaaccggaaa cccggttgac tttggccccc aaacgactgc
    33361 cgggacctat accatactgg ctgtgaacca aaacagtttt tgccagtcgc tgatgaacgg
    33421 ttcgctcgtc atgaatccga atccgtcggt attcagcctg acgcctgccg gttatatttg
    33481 tgcaggccaa tccatcggct tatccggatc ggaaacnnnn nnnnnnnnnn nnnnnnnnnn
    33541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33601 nnnnnnnnnn nnnnnncgcc tgccggttat atttgtgcag gccaatccat cggcttatcc
    33661 ggatcggaaa ccggtgtttc gtaccagtta taccttggca ccactacagt cggatcgccg
    33721 gtagccggta cgggaagtgc gctcgacttt ggccctcaat cgattacagg cgtatatact
    33781 gttgttgcca ccaatccggc aacgggatgc tattcccaaa tgtcaggcgc tgtcaacatc
    33841 tatcctcagc ctactgtctt tacaatcact tccacaggat caggctgtcc gggtacagtc
    33901 ataggactta gcagttctca gcttaatgtt gattatgttt tactgctcga tggctcagtt
    33961 cagatcgata cgatctcagg aaccgggaac ccgattgatt tcggctccca gatgaccacc
    34021 ggaacttata ccatactggc catcagccag agcacgaatt gccaggaaat gatgagcggt
    34081 tccttcgtca ttaatcccaa tcccttcgta ttcaatattg tacctgccgg tactatctgt
    34141 gtcggccagt ctgttggttt atcaggatca gaaaccggtg tttcgtatca gttatttaac
    34201 ggaacgacgt tgattggcac gtccatagcc ggaaccggaa atgcactgga ctttggtccc
    34261 caatggattg caggcaccta tacggtgata gccgtgaatg acgtgaccgg atgcgcttcc
    34321 gaaatgaacg attccacggt tttcaggcct tttccaacct cttatgccat ttatccgcag
    34381 ggcaccaatt gttccggcag taatatcatc ctgaatggtt ccgaatcagg gatcacctat
    34441 atattaatgc tgaataatac ttttccggtt gacaccctga ccggaacagg cggaattctt
    34501 gatttcggtg ttcaatacct ttcgggcatt tataccgtta atgccagcgc aacggccctc
    34561 acctgccagt cgaccatgag cggctcaaca attattgtaa acaacccgtt ggtttataat
    34621 gtaattccgg ccgggactaa ttgcggcacg gcaaatattg gtctcgacgg ttctgaaacc
    34681 ggcgtgaatt atgaattatt cctcgacgga aatcctttcg gaatagttgt ggccggtaca
    34741 gggagtcctg tatcattcgg caatcagtat cccggaacct ataccgttca ggctgtcaac
    34801 acggtcaccg gttgccaatc ggcaatgaac ggaaccattg tgatcaacga acttcctgct
    34861 gctgatgcag gcgctgacga tatcatctgt gaaaacagta tcgcccaatt gtcaggagat
    34921 gcctttgaat actcgacggt actatggaca actgccggtg acggaacgtt tataaacccg
    34981 acttccctgt atgccgacta tatccctgga gtcaatgata tcgccaacag cgtcgtaacg
    35041 ttgaccctta cggcttctaa tccgctctgt ggttcagctt ccgatgatat gttgctctcc
    35101 attatccctt accccgtggt agatgcaggc cctgatgcga ccgtttgcga tgcctgtgac
    35161 tttcacatta tctatgctac ggcaaccaat acctcttcgg tttactggat tacttccgga
    35221 accggcacat tcagcgatgc tgcttcactt aatccggtgt attttccgag tgaaaccgat
    35281 tacgccatcg gttcggtaat cctcaccctc actggaacag gcattgatat gtgcggggaa
    35341 gccaacgata tcatgacttt gacatttgat ccgaatcccg gtgttgattt cacctgggga
    35401 gcatcatgcg aaagccagtc tgttgatttt gcagtagatc cggcagctac caatatcggc
    35461 gctgtggcaa gctggagctg ggatttcggc gacggcggca cctcgttgct gatgaatcct
    35521 tcacacgtgt tcccgtcatg gggtacatat aatgtgaagc tgacgacgac cgatacaacc
    35581 ggctatgtaa ggattgtgat gaatgaagta tatgtttcgc agcctcctgt agccatgttc
    35641 tcatccagtg tctccaactg ctcgaacgag gaagtccagt ttaaagactt atcgtttacc
    35701 ctttacggtt atattgaaca gtgggtgtgg aacttcggcg acggaactcc cgacgacacc
    35761 atcaatttcc ccgacgatcc gaacgttaag cacatgttcg ataccaccgg gatttacaac
    35821 gtgacgctta ccatccgcaa ttctttcggt tgcgaaaatt ccgtcatcct gccggtaact
    35881 gtaatcacgg cgcccattgc caatttctat tacacaggca attgcgcatc cacagtggtt
    35941 aatttctccg atgcatcgtt tgccaacggt tcaggaaacg tggttcagtg gtggtggaac
    36001 ttcggagatc ccctgtcggg ctctgatgac aattccgaac ttgaaaatcc catccatacc
    36061 ttcaccgagc ccggtactta catggtaacc catattgtgc gcaatttcaa caactgcacc
    36121 gataccatcg tgaaacccgt ggttatcctt ccaccgctcg acgttgattt cacccatact
    36181 tatacctgta tcgacgagct tacccatttt attcccgata ccacggtaat cgctgcaggc
    36241 gctgttcaaa gctggttctg ggatttcggc gacgggctca ccagtaccga tatgtatgcg
    36301 tcgcatgcct ttacttcgac cggtctttat catgttacgc ttaccatcac cgacaccaac
    36361 aactgtgtag catcgggatc acacgacatt accatttacg cgttgccgat cgccaatttt
    36421 gaaacagaac cctggctttg tgaagatacc ccggtcgaat tcaccgattt gagttctgcc
    36481 gcttccgggt atatcatcaa atggttctgg gattttggca atggtgatac tttgaccgtt
    36541 aattatcccg acaaccccaa tattcagtat acctatgcta taccagggac ttacaacgtg
    36601 acattgtcga taaagtcgac cgacagttgt acggcagaga tcacgaagag cgtcatcata
    36661 ggctctacac cggttgccaa ctttaccttt gacaacaatt gcgaagataa cctgacccag
    36721 ttcaccgatc tttcacagtt gaacggaggc ggtaatatca ccggctgggc atggaacttt
    36781 ggtgatccag catcgggaat tttgaataca tcaactgttc agaatcctgc tcaccagttt
    36841 gcttcgaccg gagtatacaa tgtaaccctt accgtgacaa catcaaacgg ttgtgcgaac
    36901 gaggtaatcc ttcctgtgac ggtaatcccg gcaccgacag ttgacttcta tgcacagcag
    36961 acctgtgccg gaacatcaac cctgttcata cccgcatcaa ccatggccat tgccagtgtt
    37021 gaaacatggt tttggaattt cggtgacggc agtacctcaa acgagcagga tcccggccat
    37081 atttatccgg ccagcggaaa ttatctcgta acccttacca tcaccgatac caacggttgt
    37141 tcaaacacgg ctgcccataa tattgccatt gccccggcgc caacggcaaa tttcatcacc
    37201 agccagcctt cctgttccgg aagcgccata agctttacca gccttgccgc tacacaattg
    37261 ggttatatag tgaaatggac ctgggatttt ggagatggcg cctcgcagac gatcactttg
    37321 ccgggtaatc cgaatgtcag ccatatttat tcagattatg gactgtacaa tgtgacgctt
    37381 actgttgtaa ccaacgacag ttgccaggga agtacaagcc tgccggttca ggtgcttcaa
    37441 agcccggttg ccaatttcag ttctgacggt tcctgcatcg gcagccccgt tcatttcaac
    37501 gacctgtcgc agggtggaat gattctcacc tggcattgga atttcggtga catgtacgcc
    37561 ggcccggcca atacatctgt tctgcaaaat ccgcaacata cttatacatc ggcaggcaca
    37621 tacccggtaa cgcttattac agaaaacgtg aatggttgtt ccgacaccat tatcaggaat
    37681 gtgttgattt atgctgcgcc tgccgtcgac tttaccttca atgcaagttg tgctaacgat
    37741 acggttcatt ttatcagttc tgcatcggtg aatgtggcag caacagccgc ctggctatgg
    37801 caatttggcg atggcgcaac ttcgaccgac accgatccgt atcatgtgta tgcagcccag
    37861 ggatattata atgtaaccct caccattacc gatactgcag gctgtgagaa ttccaagacc
    37921 cgtaatgtgc cggtcactca ggctccggtg gcagccttta ccgctacgac gcctgcctgc
    37981 agcgggatgt cggtaacatt caatgatatt tcaaccacga tgaacggaac catcagttca
    38041 tggcactgga atttcgacga tggaagcgac accaccattt tcgccccggc aaatcccgac
    38101 ctcgaacatg tttttgctca ggccggaacc tataatgttt cgctgtatat tactacgaca
    38161 accggatgtg aagatgaata ttccctgccg gtgaatatca aattcagccc tgctgctgca
    38221 ttcagcgtac aaggcaactg ccctgtacag ccggctatgt ttaccgatga atcgcaggct
    38281 ctggggggat cggcaatcgt tgcctggtcg tggagttttg gcgatccggc ttcaggtagc
    38341 aatacgtcgg tattacaaaa tccgcagcat atttacagcc agcccggcac ctataatgtg
    38401 acgctcgaag ttgaaaacat gaacggatgt acgaattcca taacacagcc ggttactgtt
    38461 gaaactcctt ctgccgttgc cattgccgat ataatgactt cctgccttgg cacaccggtg
    38521 gttttccagg tcgataccac cgttaccaat ttagctgata ttaccgctta tgactgggat
    38581 tttggcgacg gaaccgctca ctcaccgatg gctaatccaa gccatgatta tacgaatgcc
    38641 ggcacttata ccgttacgct cgtgatcacc gatatcaacg gctgcacgaa ttcggacagc
    38701 cagccgatga ccgttcatac gctgcctgta gctcaattct caagccaaag caattgtctt
    38761 gagagctata cggtcttttc ggatgaaagc tatgttcccg acggcgaccc gattgtttca
    38821 tggaactggg attttggcgt aacaggcagc accaacgata catcgtcact gcaaaatccg
    38881 gtttggatat atgctggtac cggcacctac caggtaaccc tccaggtaag tacactcagc
    38941 ggctgcacgg caactgtaat ttcggaagtg accgtaaaac ccgcgcccgt agccaatttc
    39001 acctatatca ccgaaccttg tcatcaggga tccgtttcct tcaccgattc gtcgtatgcg
    39061 cagcagtcag tcgttaccaa ctggtactgg gaatttgaat cgaacaacta ttcaacgctt
    39121 cagaacccga cctatgtatt catgaataca aacacctact acgacgttaa gctgattatc
    39181 accaatgccg aagggtgcac cgatacaata atcaaaccgg tgtatgtgcc ttcgggactc
    39241 aacatgacgt tcagttttga tgaaacgtgt ttcggcgaca caacccggtt cactccgcaa
    39301 ttactcccgc cggtgctcga ttccatcgcc acttatgcct ggagtttcgg cgatccgaat
    39361 accggaatct ataatcagtc gaacctgcgt tacccgaccc ataaattctt aaaagccggt
    39421 acctacgtgg taaccctgac ggtcaccact acttctttct gcacagctac tgttaaccgg
    39481 cagattgaaa taatagcgct tcccaatccc aaatttgaat acaccggcgg cgactgcaat
    39541 gatgtagttg aatttcagga taagacatcc ggcaacaacc ttacgcaatg gatttgggaa
    39601 ttcggcgacg gaacgtccat gaccatcaat gccccgtcca gtcccgatgt tactcattct
    39661 tatcccaata cagggctcta ctttgccacc cttacggtaa cgaatgctca tggttgcgtc
    39721 aacgcagtta ccgattcggt caagcggtta ccctgcattt ccagtgaatt tactataaac
    39781 gataccactg cctgccagaa ccggtcgatg cattttaccg atatgagtac ctgcgctgga
    39841 cccattgccg gctggatgtg ggattttggc gacggcacaa cccaaaccta tacttcacag
    39901 caggctgttg tttcgcacac ctatgcaacg gcgggttcat atacggttcg cctgatcgtg
    39961 aatacacagt tggtcggagg tattattgct gccgatacca gtacaaaacc attaaaagta
    40021 ttcccggctc ccaaagcgga attcatatgg aatgacatct gcctcggcac taccacggaa
    40081 tttaaaaata ccaccaggga aaacggcttg aaggttaatg actatacatg gaattttgac
    40141 gatccgacct caatttttga taccgcttcg atgataagcc cgacttatct gtacagtgaa
    40201 gcaggcggtt ataatgtcag gttggttgcc ggcaatacca tgggttgcac cgataccatc
    40261 gttcagaccg tgaatgtttt cgacagcccg aaagcagctt tcgactggct cacttcctgt
    40321 gacggcgacc cggtgcaatt catcgataac acggatacgg catcggcaaa aatcagcacc
    40381 tggaactgga ttttccacga tgaggcaggc gctgtgatcg gtgcttccac caaacagaat
    40441 ccgtcgttcg actttacccg cgctggattg ttctcaaccg aactgagcgt taaagataaa
    40501 cacggttgca caggcaatac tgtaaaccag attgccataa acagcagtcc ggttgccgca
    40561 ttcacaatca aagacaatta tgaagacacc cagggacaga tacagttgtt aaacggtacg
    40621 ctgaatgcaa caaattacct gtgggacttt ggcgacggaa aaacctcatt ttctgaaagc
    40681 ccggtgacta cctatgataa gggcggatcg tatacaatca ccctcgttac atggaacggc
    40741 cagaactgta ccgataccct gacaatgaat tacgaactga catttaaagg gttatacatc
    40801 ccgaatgcat tttcacccga cgacccgcac caggaagtca ggattttcaa accggttggt
    40861 atgaatctca aaaagtaccg tatcgaagta atcgatcgtt ggggaaatac cctctggacg
    40921 tcagataaac ttgacaagaa cggctgtccg gctgaaggct gggatggaac cctgaacagt
    40981 gttcccgtac aatcgggggt atatatgtgg agggccagcg ccatttttat agatggtacc
    41041 aaatgggatg gcgtgaatgt gggtgataaa acctgtctgc cgcaaacaga taccggcact
    41101 gtaacattaa taagataaaa attagaaaag taaaaaaatg ttgtactttt atcctctttt
    41161 cttaaaaaca aatccagata actatgtctc agcaggtttt caggattccg gaaacctgac
    41221 cgtatcaaaa aataatgatg tgatgaaacg atactccctt acctgcaaca taactgccca
    41281 tgtagcactt atcttgcttg cactcgtttc ggtgctgagc ctgaaagcgc aggatcctca
    41341 tttcacccag ttttacaaca atccgattta ttacaatccc ggatatatcg gccttaatcc
    41401 tggttacagg gcaaggttta actatcggga ccagtggacg ggacttccca ctgatttcaa
    41461 aacttacagc ttctcgatgg atgttgcgga gcggggaatt ccgggatcgg gcggattggg
    41521 tatgctggtg ctttccgata aagcaggaac cggtctgata aaaaacaaca gcgttggcat
    41581 aggcaccgct gcccgtgttc ccctgcagga gaatatggtc acccaggttg ggttcatggt
    41641 ttctttcgtc caaaaatcga tagactggga tcaccttgtt tttacggatc agttaagtgc
    41701 acgatatgga aatatatatc ctacgaactt cgaagctcct gataaaaaca atgttaccta
    41761 tcccgacttt tccgtaggag gcgtgtatcg ctttgccgaa accggctgga atttttctgc
    41821 aatccaggga acttttggcc tggcgctgca ccatgcattt acacccaatg aatcattcct
    41881 tgggcttacc gccactttgc cgcgcaagtt ggttgtcagc ggcgacctgg tactcgaaat
    41941 tgaacagggc cgcggatcat catacaggaa ttaccgcaat acaggatctt tcaaatttaa
    42001 tccgggtttt atttacgaaa agcaagctga attctcaacg tactccgttg gggttaacgt
    42061 cctgaagtcc tctatctatt tcggtatttg gttccgtaac cagacctttg acctgttcaa
    42121 ggccaatgac gccatgttct cggtaggtgt aaatgcgcct tttaaccgtg aatcgcgttt
    42181 gaaaatcatg tatacctacg attatatcat cacggaactc cgtacggctg cccgtgcttc
    42241 gcatgaaatc tcgctcgttt ttgaattcga cagcttcagc ttgttcagca atggcggcgg
    42301 tggtggattt gcgcccagtt acagggggtc gaaattccgt gaaatggaat gctgcccgtt
    42361 ctaatcgggg taatttaaaa tattatacgg ggtgttgcct gcaggcagca ccccgtttct
    42421 ttatgccatt tggtaatatc aggatgatca gagttcatat ccgaaacgtc ttaactgtct
    42481 ttcatcttcg cgccagtcgt cgctcacttt tacgctgagc tcaaggtaga ctttagtgcc
    42541 gataaaaact tcgatatcct tacgggcatc tattccaagt ttcttgatag ccgcaccttt
    42601 atgcccgagc aggatggctt tctgggtttc ccgttctaca aaaatggttg ccgcaatcac
    42661 ggtcatcttt tccccttcct tatatgattc aacgaccact tcggtggaat agggcacttc
    42721 cttggtgaac tgaaggaata ttttttcgcg gatgatttca gaaataaaaa aacgtaacgg
    42781 ccggtccgat agttcatcat ctttgggata ataaggcggg ttctcgggta aaagctgcag
    42841 gacggtttct gatatctttc cgatattata gttcagtttg gcggctatcg ggatcacttc
    42901 ggcgccgggg aactgttctt tccagaaaac gactttctgt tcagcttctt ccggcgatgc
    42961 aaggtcgact ttgttaataa tgaggatcag cggctttcct gatgcttgta tcttccggat
    43021 aacatcttcc tcgaaagtgt tttctcctac ttcggtcata tacaggaata catcggcatc
    43081 ctgaagggca ttatcgatga atttcatcat cgatttgtgc agcttgtaat gcggtttcag
    43141 gataccgggg gtatcagaat atattacctg ataatcgggg ccgtttacga ttccgagaat
    43201 acgatggcgg gtagtctggg ctttagccgt gatgatggat agtttctcac cgatcagcag
    43261 gttcatcagg gtggatttgc cgacattggg atggcccagg atatttacaa aaccggcttt
    43321 atgcgtcatt taggaaatat tttcgtgaaa atttggatag caaaggaaca aaatatacct
    43381 ttgcactccc ttaataaaac gttcattcat ataacaaaga tatactgcgg ggtggagcag
    43441 aggtagctcg ttgggctcat aacccaaagg ccgtaggttc gaatcctgcc cccgctacaa
    43501 aggaaccccg gcgctatgcg ccggggtttt tatttttccg ccggagctgc aaacttgttt
    43561 gcaaagcgac gaaggaaaaa taaaaacccc gtgcgaacgc agttcgcggg gttccgttgt
    43621 aaagctcccc tatttcagga tcacgcaagt aatccaacaa tccggatagt tttgaaccgg
    43681 ataagcataa aaccccgtgc gaacgcagtt cgcggggttc cgttgtaaag ctcccccctt
    43741 caggatcacg caagtaatcc aacagtccgg atagtttgga accggataag cataaaaccc
    43801 cgtgcgaacg cagttcgcgg ggttgcgttg taaagctccc ctatctcagg atcacgcaag
    43861 taatccgtca gacctgatag ctttgaaccg tataagcata aaaccccgag cgaacgcagt
    43921 tcgcggggtt ccgttgtaaa gccccccctt caggatcacg caagtaatcc aacagtccgg
    43981 atagtttgga accgtataag cataaaaccc cgagcgaacg cagttcgcgg ggttccgttg
    44041 taaagctccc ctatttcagg atcacgcaag taatccaaca gtccggatag tttggaactc
    44101 cgctcacgcg cgtctcctga cgcgtgtgtt gagactttac aacgctgtaa aaaatttgtt
    44161 tacttttatt gcatggccag caatatagga tatccggaaa attaagtgaa aaagaatctg
    44221 tggtgcacac gtcaggaaag gcgcgcgagc cagggcgaag tgtattctgg aaaggtcatt
    44281 aaaatcaaat agctttgaaa aggacctccg ctcacgcgtc tcctgacgcg tgcattgaga
    44341 gatgtccttc ttaccacgga ggcatcgagc gcactgagat tcacagagaa aacatgcaat
    44401 tctctgtgtt tcttcgtgct attgagcggt aaaaagaaga aaaccatgga gaatccatgt
    44461 aaataaaaaa agggggcctt ggtcagataa gtggtcatta gatgccacaa ttatctttgt
    44521 tgataatact ctaaaccaag gccctatgac taaggtttac taaggacaat taaatgatct
    44581 gtgttccttc acattcctga gtggtaaaaa aatgagaacc acggaggcat cgagcgcact
    44641 gagattcact gagaaaacat gcaattctct atgttccttc gtgttcttga gtggtaaaaa
    44701 gaagaaaacc actgagtcac ggatcacaaa ggttcattaa ggacaattaa atgcactatg
    44761 ttccttcacg ttcctgagtg gtaaaaaatg ag
//
LOCUS       DAOPYV010000017        44695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_698_length_44695_cov_10.992003, whole genome shotgun sequence.
ACCESSION   DAOPYV010000017 DAOPYV010000000
VERSION     DAOPYV010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 44695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44695
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_698_length_44695_cov_10.992003"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PKI35_03585"
     CDS             <1..575
                     /locus_tag="PKI35_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43495.1"
                     /translation="NSQDEDICTVQNSEFIDICGDTIIYTINVFPKTNLSNKERTTNI
                     KIKARIGDKPEEKSINIIQEGSPAITLHLEADLMIDGVTTHYSSDALLYPDGTAYIPG
                     FKWPELGWGTYWDRFGFCYSTTFSQTGFEQPWGWEDTLLIHLLTSNPNNLEIGELRDG
                     TIEFSGYSWAGLTGYRVGTFGGTFTLKRTE"
     gene            776..2755
                     /gene="rpsA"
                     /locus_tag="PKI35_03590"
     CDS             776..2755
                     /gene="rpsA"
                     /locus_tag="PKI35_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNX43496.1"
                     /translation="MNNDVNVSDELKNEGETENNATENAETAEVASEETAETKTAEVV
                     EAAVETEVPVKEKKAAEEPVVAAPAEFDWNSIGKKHEAYPAAERNRLEAIYDKTMSSI
                     GDHEVVDGLVVGLTNREVVVNIGFKSDGVIPVSEVRYNPDLKVGDTIEVYVENQEDMG
                     GQLQLSHKKARILRSWERVNEAFEKGEIINGFVKCRTKGGLIVDIFGIEAFLPGSQID
                     VKPIRDYDQFVEKVMEFKIVKINQEYKNVVVSHKALIEDELEAQKAEIISKLEKGQVL
                     EGTVKNITSYGVFIDLGGVDGLIHITDLSWGRINHPEEFVQLDQKLKVVILDFDENKK
                     RIALGLKQLTPHPWDSLNPDLKVGDKVKGRVVVIADYGAFVEIAPGVEGLIHVSEMSW
                     SQHLRTAQDFLKVGDEIEAIILTLDREERKMSLGVKQLIPDPWTDILKKYPVSSKHTA
                     TVRNFTNFGIFVELEEGVDGLIHISDLSWSKKIKHPAEFTKVGEKIDVVVLDVDVENR
                     RLSLGHKQLEENPWDVFESIFTVGSVHKGTVVSNAEKGVVVSLPYGVEGFAPSRHIQK
                     EDGSLAKIDDTLDFKVIEFSKENKKIILSHSRIHQDAKQAEKVKEENEKESAEMSTKR
                     AVKKIKDNLEKTTLGDLDALANLKASLEKKEEENK"
     gene            2873..3349
                     /locus_tag="PKI35_03595"
     CDS             2873..3349
                     /locus_tag="PKI35_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43497.1"
                     /translation="MYLGFGLQRWIYHQRPRKIFTRVKPPLPVEDAELLTGAAPDVPE
                     LPGVIAERNIELLKKDTRSIKTTNRIIISLLTVAITATLIFLPFRIYTYFKTNDAKSV
                     QYDKKAEKQLSDAMYRYGIHLLKEGDTTKAAAQFAESLKYNPDNRRADSALRECFK"
     gene            3393..4319
                     /locus_tag="PKI35_03600"
     CDS             3393..4319
                     /locus_tag="PKI35_03600"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNX43498.1"
                     /translation="MIRFSVTILGSSSATPTSERNPTSQLVNHHDRLFMIDCGEGTQV
                     TLRKLKIHFQKVNHIFISHLHGDHFYGLIGLISSMHLLGRDHELHIYGPPELEELIYF
                     QLRVSQTTLIYPLVFHATRSDVPGIIFEDSFLEVSTFPLNHRIATTGFLFREKPGLRK
                     VIKEKALEIKIPAWAYEKIRKGEDFTDGEGNFYPNNELTLDPPPPRSYAYCSDTAYHE
                     PVVEYIKGADLLYHEATFMNDREANATEKFHSTAAQAGKIALQAGVKKLILGHFSARY
                     DDIQPLLEEARVNFPEAYLAEEGACFEIPVAI"
     gene            complement(4312..6552)
                     /locus_tag="PKI35_03605"
     CDS             complement(4312..6552)
                     /locus_tag="PKI35_03605"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HNX43499.1"
                     /translation="MLRKALFFTLLISGLLAYSQKQTGVAIVRDGKPMVSILISSNAT
                     QSDRTAAALLQQYIQKVSGCLLNISEKSASPSNFIAIWEADERTRQKESLKEDGFILS
                     TGNQNIFIESGGKKGSVYGVVELLERYIGCRMYAPGVEHVPHMEDITIPAMYQVQNPA
                     NSFRVVHGNFGNDTLYRDWQRLNSIDEMFADGYYVHTFNKLVPWETYFAEHPEYYSLM
                     NGKRIKDQLCLSNEDVYRLAAEKLRKEMAKQPDKKVWSVSQNDNFSYCHCDRCMKIIN
                     EEGSPSGPVIRFVNRLASEFPDKVISTLAYEYSRQAPKITKPLPNVQIMLCTIELNRS
                     KPMDEDSTSLSFCNDITDWGKIASNIYLWDYTVNFSHSTSPFPNLHVLQPNLSFLTRN
                     GAHQHFQQTNTGTGHEMSELKNYLIARLLWNPDINADSVIRDFVDGYFAGAAPYIQQY
                     IDRLTSEIKKTGEWLDIYGHPVAHANTFLSQSNMEFYNNLFNEAEAAVKDSADLLQRV
                     KVCRLPIQYAAIEIGKNDMFGPRGFYTDQNGAFVLKEQMNQLVEDFYGTCRANQVSPL
                     NESGLSPEEYYNATRRFIDVQVSGNHAFRKKVTASPLPSAKYGGGDLAALTNGVQGAN
                     DYKAHWLGWEATDFTLTLDLGTVTEAKQISIGTLYDPKSWILHPVSVTCQVSNDGRSY
                     HNIGKLVVAGDQHKEDVTRNFHFTNPSGKGRFVRFMVQGTKSLPAWHPSAGGDSWVFV
                     DEITVR"
     gene            complement(6625..7119)
                     /locus_tag="PKI35_03610"
     CDS             complement(6625..7119)
                     /locus_tag="PKI35_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HNX43500.1"
                     /translation="MNGDPRLYSILHDLGIPFEYHEHPPAPTIEIAMQYWKDIVATHC
                     KNIFFRNHKGDRHYLVVLDHRQELGIHDLEKRLKQGKLTFASQQRLMNYLGVAPGSVS
                     PYDLINDSDHHVRVFFDENLKNCEKISFHPCINTASIIVNGKDFWRFMQWTGNDFEFI
                     RLYD"
     gene            complement(7205..7939)
                     /locus_tag="PKI35_03615"
     CDS             complement(7205..7939)
                     /locus_tag="PKI35_03615"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNX43501.1"
                     /translation="MKKLSILLTTVLVIIASTFCTSQNVKKARANTLTKKEIKQGWQL
                     LFDGKTLNGWKGYNHNECGARWTVQKGDLVCLGEGGGMGYDIITTQKFSNFELSLDWK
                     ISPQGNSGIFYHVVEDAAFGNPYECSPEYQLIDDLGWPDKLEEWQKTGADYAMTIPDA
                     TVKKLNPVGEWNTSKIVFNNGHVEHWLNGVKILEFTAWNDEWNKKRTEGKWKDYPAYG
                     QARKGSIGLQNHGSMIWFRNIKIRPL"
     gene            complement(7992..8807)
                     /locus_tag="PKI35_03620"
     CDS             complement(7992..8807)
                     /locus_tag="PKI35_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43502.1"
                     /translation="MKSLNRESLRITVNSAAYYILSYLVIAFIFQFTSSAIAFYAFQI
                     PSVFHQNAADYLLKPEAWSFDSVKVVYSAGAVISLIIGLLCLIVYLKALVLDGLLRLF
                     FLWGFIHGINMFLGSVMLGAFIFEGFGYVLSWMYLQDTAKMILLFICLFLILASGSLM
                     TRPFLYSANTYYNMLTPEMRPAFRRYQFYLPYFIGTILLFLIRMPLSLYEFLLLISPG
                     LFILPLMWGINKYPPYFFEERPKKIEVHWRPVIITIVVIILYRMVLGIGITID"
     gene            complement(8912..10162)
                     /locus_tag="PKI35_03625"
     CDS             complement(8912..10162)
                     /locus_tag="PKI35_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX43503.1"
                     /translation="MDIFEKRVKNLGPLGQYGKDADGYFMFPKLEGEIGSHMIFRGKE
                     RLVWSLNNYLGLANHPEVRKADAEAAARWGLAYPMGARMMSGQTDYHEQLERELAEFE
                     GKESAYLFNFGYQGMVSVIDSLVDRNDVIVYDSEAHACILDGMRLHIGKRFVYPHNNI
                     ENLRKGLERATRLTEQSGGGILVITEGVYGMSGDLGKIKDIVELKKEFDFRLMIDDAH
                     GFGTMGPTGRGTGEHFGVQKDVDLYFGTFAKAMAGIGGFIASNARIIDYLKHNMRSQI
                     FAKSLPMPMVIGALKRLELVRTQPQLRENLWKVVNAMQTGLKNEGFNIGNTESPVTPV
                     FLNGTPEEATQVILDLRENFNIFCSIVTYPVVPRGVILIRLIPTAVHSLEDVNYTIEA
                     FRVIRKNLEAGKYTSNTIADFSKV"
     gene            10404..11192
                     /gene="bamD"
                     /locus_tag="PKI35_03630"
     CDS             10404..11192
                     /gene="bamD"
                     /locus_tag="PKI35_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNX43504.1"
                     /translation="MVKKLLLYSMLIFMIVLSSCSKYQKLLKSSDNDLKYDKAIGYYE
                     KGEYFRAQQLFEQIFSFFRGTEKAEKIAYLNSYCYYKQKDFIMGGYNFKNFAANYPSS
                     KYAEECAYMSAYCSYLDSPRSSLDPTSTNDAIAALQLFINQHPESERVEECNNLIDEL
                     RGKLEKKDYDIATMYYKMADFKAAIITFNNVLKDFPDTKRKEDILFQILLAKYDYAIN
                     SVESKKKERLNDALESYKLLTSEFPDGDFTARARSIEKNIMKLM"
     gene            11219..11551
                     /locus_tag="PKI35_03635"
     CDS             11219..11551
                     /locus_tag="PKI35_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNX43505.1"
                     /translation="MDYKKLKIDTTTVTRNVGELYAPTENIYETVAILAKRANQIGLE
                     IKEELDNKIEEFATPTDNLEEVFENREQIELAKFYEHLPKPTLIATNEFLNGQIFFRS
                     PSKDLKSE"
     gene            11654..12862
                     /gene="coaBC"
                     /locus_tag="PKI35_03640"
     CDS             11654..12862
                     /gene="coaBC"
                     /locus_tag="PKI35_03640"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNX43506.1"
                     /translation="MLKGKKIIVAITGSIAAYKIPFLVRLLIRDGAQVKVLMTPSACD
                     FVTPLTLSTLSQSPVYIKPFNESDGSWTSHIELGSWADVMLFAPLSANTMAKMATGIT
                     DNLLTAVYLAARCPVFFAPAMDVDMYNHPSTQANIKRLQSFGNILINPREGELASGLS
                     GCGRLEEPENILKVLHRFFANKASLAGKKVLVTSGPTIEPIDPVRFISNHSSGLMGNS
                     IAVEAAQRGAEVALISGPVHDLPVHPNIQTIKVSKAGEMLEETVSRFGQADIAIMAAA
                     VSDYTPVNVSDEKIKKSAGEGLTLQLTQTKDILAELGKIKKGGQVLVGFALETENEIE
                     NAVRKLHTKNLDCIVLNSMKDKGAGFSHETNRISIISGKNQVCTYDLKPKTAVASDIL
                     DYITEHFLKV"
     gene            12889..13791
                     /locus_tag="PKI35_03645"
     CDS             12889..13791
                     /locus_tag="PKI35_03645"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HNX43507.1"
                     /translation="MKRSILLLLVTVLPLLMKAQEFICQVSVNAPSVEGSERKVFQTM
                     QSALYEFINNRKWTNYVYKPEERIECSMMLTITERVTSDQFKGRLNLVLKRPVYKTSY
                     NTNLLNYVDEAINFKYVEYEPLVYNDDAFTSNLTSVVAYYLYVFLGLDADSYSNMGGT
                     PYFEKAKAIVNTAQNASESGWKAFESTKNRFWLVENLLNGAYSPLREGLYQYHRLGLD
                     VMSDNIDLGRSAVSDCLDNFQRAYRQKPGLFILQLMMEAKCDELINIFSQAAPMDKTK
                     AVNILKEIDPSNSSKYQKIMSSGK"
     gene            13845..15500
                     /gene="recN"
                     /locus_tag="PKI35_03650"
     CDS             13845..15500
                     /gene="recN"
                     /locus_tag="PKI35_03650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNX43508.1"
                     /translation="MLLHLSVDNFILIRHLDIGFEDGFSVITGETGAGKSILVGALGL
                     ILGQRADTMVLHDKEKKCIVEGVFRIEGYGLEPLFTENDLDFDPVTTIRREITPQGKS
                     RAFVNDTPVNLSILNQLGDRLVDIHSQNQTLELNEAAFQLALVDSYVAKPGLLNDYCG
                     LFSRFTKAQRELNGLVERQEKATADREFQHYLLDELETASLRENEQEEAEAELVVLTN
                     AEEIKTRLYSVINLLNEFEPNMIGQLAEIRQLVDQAVHLGATLEPVSERLETCHIELK
                     DMAAELTAYASHVHFDPQRIEELTTRTDLIYHLLQKHKVQSVGELLAIKDQLSLNLEN
                     IETLQSQIDELKSEISQLEKQLIRAAHELSESRKGVIPFIEKEMVKTLADLGMPAAVF
                     TIGHEVNGEFTKSGIDKVTFLFSANKGGQPRELRKVASGGELSRLMLAVKSLVVSRSM
                     LPTILFDEIDSGVSGEIAGRIGSILHKMSATVQVIAITHLPQIAGKCKTHYLAYKEEG
                     EKSTFSSLKQLSPEDRITEIARMLSDDMITETAKAAARELIRN"
     gene            15560..16432
                     /locus_tag="PKI35_03655"
     CDS             15560..16432
                     /locus_tag="PKI35_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX43509.1"
                     /translation="MAYNLLKGKKGIIFGALNEMSIAWKTAEKAYEEGAEFVLTNTPM
                     ALRMGDTHILARNCNTIVIPADATSIPDLENLFVKTMEHFGGKIDFILHAIGMSPNVR
                     KKIPYDQINYDFYLKTLDISALSFHKVLQTANKFDALNEWGSVVALSYIAAQRTLFEY
                     NDMADAKAALESIARSFGYIYGRKSHIRINTISQSPTLTTAGSGVKGINGLMDFTERM
                     APLGNATAEECADYCITLFSDLTKKVTMQNLYNDGGFSSMGMSYRAMQMYNRSLDCKE
                     CNGHKIDEDKEDRL"
     gene            complement(16453..16887)
                     /locus_tag="PKI35_03660"
     CDS             complement(16453..16887)
                     /locus_tag="PKI35_03660"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNX43510.1"
                     /translation="MPSFPGGKSSFREFVKENLRYPEEALKNNIQGTVYLDYTVDNIG
                     VVENIAVTRGIGYGCDEEAIRLIKLITYPAVKNKGVKMKVTMKTRIAFMLPVAQTEKE
                     EISTAFRINYTATSPSGGKKPEQQPRSSYGYTITFGSQPENS"
     gene            17253..18002
                     /locus_tag="PKI35_03665"
     CDS             17253..18002
                     /locus_tag="PKI35_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNX43511.1"
                     /translation="MKVLVCISNVPDTTTKVRFLDDNKKLDTNGIQWVINPWDELSLT
                     RALELKDVAANGIEKVTVAHVGTVTSEPTIRKALAIGADDAVRVNADAADAYFVAAQL
                     AEVLKKNPYDIILCGIESSDHNGSTVGGMLAEMLDIPSVSAVSSLSVENGAAILTREI
                     DGGKEVVTVPAPFVAIVQKGIAKEPRIAAMRGIMMARTKPIAVTEPAPVEALTEVIGY
                     EMPKPRTACKFVDAENPKQLIDFLQNEAKIL"
     gene            18026..18997
                     /locus_tag="PKI35_03670"
     CDS             18026..18997
                     /locus_tag="PKI35_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNX43512.1"
                     /translation="MSVLVFVENWDGKFKKASFELISYAAGVAEMCGTQAVALSIGNV
                     GQPELDKLTGFGAAKIIAASNDKLNVLDNQAYASVIAQIAINEKASVVVLSNNNTGKA
                     LAPRLSVRLKAGIGAGVNQLPLSADPFVVHKKAYSGNAFANVLIKTPVRILTLAQNSF
                     ELKESAAKAPVEHIAVDVEAGMVKTTVKDVQKQTGKILLTDAEIVVSGGRGMKSPDNW
                     GPLTELADLLGAATACSRPVSDEGWRSPDEHTGQTGKIIAPNLYIAVGISGATQHLAG
                     VSASKYIVAINTDKDAPIFGAAQYGIVGDAAKVLPKLVEAVKEMKAK"
     gene            19117..20433
                     /locus_tag="PKI35_03675"
     CDS             19117..20433
                     /locus_tag="PKI35_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNX43513.1"
                     /translation="MVKQIVFAIALIITLLVFAYTISRIIGFFRLTRKGFPVKEIGKR
                     FGIMMKVAFGQKKIFRRPVIGLLHALVFWGFCVILFGSIEMVIDGLSGARKSLSVLGW
                     FHDLIMLSGDIFALLVGISIIVFLIRRLFLHVRRFEGIEMKKKSHTDATIALTMILLL
                     MVSLLGMNASYVESCRQSGETLYGIYPVSNLLTGAFSGMQLKNVNIWNEIFWWSHILL
                     IFIFANILPYSKHFHVFMSIPNVFLSRLDPLGKLPNMDSITHEVKLMMNPETAYAAPA
                     SDTPVERFGVKDAEDINWKNYFDSLSCTECGRCTSVCPANITGKRLSPRKVMMDVRAR
                     MKEKGPHLLKDKGYDDSRSLIRDYITEEEIWACTTCNACAMECPININHPTLIVDMRR
                     YLVMEEASAPGELKTVFNNIENNGAPWQFSPEDRLLWAENIEMNMN"
     gene            20453..21247
                     /locus_tag="PKI35_03680"
     CDS             20453..21247
                     /locus_tag="PKI35_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNX43514.1"
                     /translation="MEKVKVEVPVMADLFARGEKPEYLFWVGCAGAFDDRYKKVAAAF
                     VKILTHLKVSYAVLGKEESCSGDPARRAGNEMLYQMQALQNIEVFKAYNVQKIITICP
                     HCYNIFKNEYPDLGGHYTVINYLQFIQENIAGGKLILPQNAFDSKVITYHDPCYLGRA
                     NGIYEEPRAILEHLTRNFVEMGRNRSFALCCGAGGGQMFKEAEKGNKEIFIERTEDAL
                     NTGCNIIATACPFCMTMMTDGIKYKNHEEDVRNYDIAELIVQALNL"
     gene            21319..23103
                     /locus_tag="PKI35_03685"
     CDS             21319..23103
                     /locus_tag="PKI35_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNX43515.1"
                     /translation="MENRKVLKSGEFLVAEVEPNDVFIPEEFNEEQLMIAQTCRDFLV
                     TEVYPNLDKLDKQDRELMKEILKKSGELGLMGISLPEEYNGFGQSFVTQMLTAETIGA
                     GYSFSVAFMAHCGIGTLPIMYYGNEEQRQKYVSRLATGELIGCYCLTEPGAGSDANSG
                     KTNAKLSEDGSHYILNGQKMWITNAGFADTQVVFAKIENDRVLSAFVVERTMPGVVVA
                     PDEKKMGIKGSSTAQIFYNDVKVPAENLIGKRGEGFRIALSILHMGRLKLGANVIGAA
                     KKAITDSVKYANERKQFGVLISTFGSLKYKMAEQVIKTFGHESATYRASYDIDHLIGE
                     LKATGMDKGRATIEAISHYAVEAALLKVYGSEMLDYVADEAVQIHGGMGYSAEMAVER
                     GYRDSRINRIFEGTNEINRLLVVDTAMKRAMKGDFDLFGEAEKLAADLGSVACKILGN
                     ETYYEEKSVYLKNFKKVALLAINGASKHFGKQIAQEEEVLNNISDIMMQIYVAESMSL
                     RIQKLEAARGADAVSVYRDILDVFVYDSADLVRKSAIDAVNAFATAELLPALHSAVDS
                     LTRVAALNVKESRRRIADKLIADNEYKF"
     gene            complement(23177..25780)
                     /locus_tag="PKI35_03690"
     CDS             complement(23177..25780)
                     /locus_tag="PKI35_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNX43516.1"
                     /translation="MEAKFSQRVKDVLSFSREEALRLGNDYIGVEHLFLGILREGEGL
                     AIKILNYLNVDLAEIRKTIESTIANRSKKIDIEELKNLPLVKQAERALKLTYLEAKLF
                     NSDLIGTEHLLLAILKDEDNIVTRSVLKYGVDYDIVKEEVKSYLVSLDEDASRGVDRP
                     EKDSRTDFHDELPGGAQDDDTDEGSPFGGSKKIADSKSKTPVLDNFGRDLTRAAEENR
                     LDPIVGREKELERISQILSRRKKNNPILIGEPGVGKSAIAEGLALRITQRRVSRALFN
                     KRVFTLDLASLVAGTKYRGQFEERMKAVLNELEKNRDIILFIDEIHTIVGAGNASGSL
                     DASNMFKPALARGEIQCIGATTLDEYRQYIEKDGALERRFQKVLVDPTSPEETIEILN
                     QIKEKYEDHHLVKYTDEAIDACVFLTNRYVTDRCLPDKAIDALDEAGARVHISNMHVP
                     VEIVNIEAQIEEVKQRKMKAIKAQKFEEAANYRDTERRLQNDLESAKRRWEDESKINR
                     ETVSAENVAEVVAMMTGVPVQRIAQNESDRLLNMENELGGAVIGQDEAIKKVVKAIRR
                     NRAGLKDPNKPIGTFIFLGPTGVGKTHLAKVLAKYLFDSEETLVRIDMSEYMEKFAVS
                     RLVGAPPGYVGYEEGGQLTEKVRRRPYSIVLLDEIEKAHPDIFHILLQVLDDGVLTDS
                     LGRKVDFKNTIVIMTSNIGSRQLKDFGTGVGFSTVAKQSQWSNQAQGVIENALKKTFA
                     PEFLNRIDDIIIFESLGRDDIHKIIDIELRQLFDRIRKMGYQPQITEAAKDFILDRGW
                     DEQFGARPLKRAIQKYIEDTLAEEIIKSTLKEGDTILIDHEKDAGELSIKVLPSAKLE
                     IPPVTGESVTG"
     gene            26050..28608
                     /gene="gyrA"
                     /locus_tag="PKI35_03695"
     CDS             26050..28608
                     /gene="gyrA"
                     /locus_tag="PKI35_03695"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNX43517.1"
                     /translation="MDYNDEGEKIVQVNIENQMKSAYIDYSMSVIVSRALPDVRDGLK
                     PVHRRVLFGMFDLGILSNRSYKKSARIVGEVLGKYHPHGDSSVYDAMVRMAQDWSLRY
                     PLVDGQGNFGSIDGDSPAAMRYTEARLKRLAEELLADLDKETVDFKLNFDDSLEEPTV
                     LPSKVPNLLINGSSGIAVGMATNIAPHNLGQVVDGTIAYIDNHDITIEELMQYVKAPD
                     FPTGGVIHGYDGVREAFETGRGRIIIRGETTIETDDKGHECIVATSIPYQINKAEMIK
                     KTADLVNEKKIEGISDIRDESDRNGLRIVYELKRDAITNIVLNKLYQYTSLQSSFSVN
                     NIALVKGRPVQLNLKQLISMFVDHRHEVIVRRTQFELREAEKRAHILEGLLIALDHLD
                     EVIALIRASVTPDEAKTGLMERFGLSEIQAKAILDMRLQRLTGLERDKIKEEYAELIK
                     LIQHLKDILADESLRMQIIKDELLEIKEKYGDEPRSRIEYTAKDFRIEDTIADESVVI
                     TISHMGYIKRTMLTEYKRQNRGGRGARGSDIREEDFLEHLFVATAHNYLLFFTEKGKC
                     FWLRAFEIPEGGKASKGRAIQNIVNIEPDDKIRAIINLKDLNDDEYVNNNFIILCTKQ
                     GTIKKTSLEAYSRPRANGINAINIVDDDCLLEARLTDGNCEIMMALRSGRAIRFNEAT
                     VRPMGRNATGVRGIRLAGDHDEVIGMICGKDENVDILVVSENGFGKRSSLADYRVTNR
                     GGKGVKTINITEKTGQLIAIKNVTDNDDLMIINRSGIIIRMAVSDLRVVGRATQGVRL
                     INLKENDAIAAVAKVECEEDDNLVGQNEEEPGDDNTYELTDIPDEENDEPSEKE"
     gene            28707..29876
                     /locus_tag="PKI35_03700"
     CDS             28707..29876
                     /locus_tag="PKI35_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX43518.1"
                     /translation="MKKSILLLALVIMTSMTFAQFKKDRTSAYNYWQKGDLTKARDYI
                     DKAMAYPEAATDAKAHYYNGAIYLDIHFKPEMKETVPNDLQIAYDEMKKAQELDTKGD
                     FKSDIMIRMLTLGGQFFNAGADLFKAEKFAEALSNFNQAVKIASDNQSLDTLAIYGTA
                     LCYEKLKDNENATATYEKLISLSMKEPSIYTSLASIYIDMKMMDKADKVLQDGLRLFP
                     GNYDLIISMANLYIKSNQHQKALESLTIAKEKDPTNKTIAFALGVTYDLLAKDTNLTV
                     ADRKIYFDDAVKAYEETIKLDPNFFDAQFNLGVVYYNKGGDVINEANKLPLNDPNYEN
                     LVNQGKEYLNKALPYLEGAEKMNPGDRDTLISLKEIYTRLGMYDKLKTVNDKLNQ"
     gene            30015..31025
                     /locus_tag="PKI35_03705"
     CDS             30015..31025
                     /locus_tag="PKI35_03705"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNX43519.1"
                     /translation="MKVFKIILWALLIFILAVFLTGFFLPRTVSVSRSVMIKGSVDDI
                     FEQVNTLKNWEKWSPWHHLDTTMTILYSGPGKGIGASYSWKSGNKNVGTGKISIINSF
                     PYDSVILDMDFMENGKSVGKFIFSKADTSHVFVTWTMTSDLGKNPVIRYFGLLMDRFV
                     GPDFEKGLQNIRHVVESHPETTKPFKITETDIPARIMLTIRDTCSGKNIRPKMGFIYG
                     EIFNIIKQKHLQVTGFPVAIFHTISPALFDIEAGVAVNKIVSIEKNNVQFTEMPQQHA
                     VMAQYFGPYEGTAQAYAEIEKYITANKLSVTAPVIEEYISDPKTLPDTAKWQTNIYLP
                     VK"
     gene            31168..33198
                     /locus_tag="PKI35_03710"
     CDS             31168..33198
                     /locus_tag="PKI35_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HNX43520.1"
                     /translation="MAAKVVRVPRLVEVDPWLIPNTNDVISRIDRFRSRLASIETDFG
                     SLLKFASAHDYYGFHFDKQNKRFVYREWAPAAHQLYLTGDFNHWHGTSHPMWRNDYGV
                     WEIFLDFETYKDRFKHGSFVKVVVLSDLGLQKRIPAYIRRVVQDEVTKDYTGQLWFPS
                     KFDWKNDNFSIKNGKDLFIYEAHIGMAQEKEGIGTYQEFTDNILPRIKKAGYNAVQLM
                     AIQEHPYYGSFGYHVSNFFAPSSRFGTPEELKLLIRTAHSLEIAVIMDIVHSHTVKNI
                     NEGLNLFDGSENQYFHPGARGEHPTWDSKLFDYGKTEVLRFLLSNVKYWLQEFHFDGF
                     RFDGVGSMLYFHHGDETIDSPDKYFRQGVEYDAVTYLQLANHLAHKVKPDAVTIAEDV
                     TGMPGLTAPVPDGGIGFNYRLGMGIPDFWIKYLKDLRDEDWNIFEMWNVLNDRLPYVK
                     TVAYCESHDQALVGDKTLAFRLMDKEMYFSMRNSDHNPVIDRGIALHKMIRLFSISLG
                     GQAWLNFMGNEYGHPEWIDFPRQGNGWSYKYARRQWSLVDDKTLKYHFLADFDREMIK
                     IMAEYRIMSSGYARQLNMDEANKCIIFERGGLLFLFNFHHQRSISDYSFIIPGDGRFQ
                     ILLNSDSKLFGGHGRIDDHTVHNALFDKENNCYRLQVYLPCRSALILQKTVV"
     gene            33293..33619
                     /locus_tag="PKI35_03715"
     CDS             33293..33619
                     /locus_tag="PKI35_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43521.1"
                     /translation="MSNKPAKKRVIVSYQNLSSELKEAVLKKYPLGWVNNMIKVKGAG
                     DTFFHAITLDTDEISYLIKVPVRIDQKSDKDDEDFFAESIDIKESDESSGGEGKEDDN
                     EQDREE"
     gene            complement(33788..35602)
                     /locus_tag="PKI35_03720"
     CDS             complement(33788..35602)
                     /locus_tag="PKI35_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNX43522.1"
                     /translation="MPLTRVDRIREYINIRLYKHKVYVIRSLQILSIFVAIVAVGSII
                     YYYGFPKTPATYRLNYIIMEGSFIFYFVKFLTKIFYDFHPIEFIKNNLFEAIIMLGLL
                     LDGIGLMLFGDHIVLMFFKLLGLPKFTEYFIVFIQLYFFVFIAFEVGRASEHISLIKM
                     GPSAMLALSFIILILTGAGLLCLPEMTVDRIRFIDALFTSTSACCVTGLVVVDTATCF
                     TIKGQAIIMLLIQLGGINIISFAAFFATFSRSSAGLRYQSLIKDLVSTDQLSETRTVL
                     QKVIFYSLFIELVGATLIYFLWPGEISFQGKGGKAFFSLFHSVSAYNNAGFTLFTDNL
                     YDVGVRHAYNLQLIIAGLIFLGGIGIMVLEDMFSIARIRERRKMKWKKLRVHSKVALF
                     TSFGLILAGAVVFYFVERHRTIEDYGYYGAIVTSVFQSVTCRTAGFNSVDFSHLSQPV
                     LMFMIFLMFIGASPASTGGGIKTTTFATLISSAYATIRGKKNVELMHHTVSFELIDKA
                     YSIALFSVSLIFISTFFLSITDGNHPFLNLIFEEVSAFGTVGLSTGITPFLSDAGKTI
                     LIFTMYVGRIGTLTLAMALLRKVSYSNYRYSEISLLVG"
     gene            complement(35618..36313)
                     /locus_tag="PKI35_03725"
     CDS             complement(35618..36313)
                     /locus_tag="PKI35_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNX43523.1"
                     /translation="MATKKYAVIGIGQFGKAIAKNLSAKGYEVMAIDNNQEVIDSIGE
                     DVAYAVALDATDKKALQSQNISEFDAVVVAIGSDFEERLLCSSVLLDLQVKRLITRAN
                     GEAQRIILEKMGITEILSPEEEVGVIVAERLSNPSIVSYLQLPDDYRIAEIIAPKNCI
                     NKTFGEIDLRDKYKLSLITLKKEIHTVKGGKELAEHHIIGVPETKQILEVTDRLLIFG
                     HIKDIDRFLQINA"
     gene            complement(36716..36898)
                     /locus_tag="PKI35_03730"
     CDS             complement(36716..36898)
                     /locus_tag="PKI35_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43524.1"
                     /translation="MKPELSERTTRMKQERAGTRQNSACIASSENGTSYGKEPRKIFL
                     PPGTVSKGNTASLCKK"
     gene            complement(37329..37796)
                     /locus_tag="PKI35_03735"
     CDS             complement(37329..37796)
                     /locus_tag="PKI35_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43525.1"
                     /translation="MTSYLYPDNELKLVEGNSYIFKVLKLITLQDSEQYLIVEDPFNL
                     RHFIPAKPYEKYGIQIGSEINCYVDHINCTGRVFLEPDHPVYRIGEKYTFRITGILSS
                     QNKDIKLKTSDCFGNNLEIVVPETAFLQNKKEIICAVKRIKRGIPDLNILNEK"
     gene            complement(37852..40053)
                     /locus_tag="PKI35_03740"
     CDS             complement(37852..40053)
                     /locus_tag="PKI35_03740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNX43526.1"
                     /translation="MKNTTQVRAQGENNTVYRSYRLLMLSCDRMADTDQKKQIRRAYK
                     YMISVFQNETPEVCQSRVHHSCAVASILAMEMEMPVSCIIAALLQDTLTNEKSLAEIE
                     KNFGDNTSGLVSQLHRISGLHTEKISIQAENFIQLILTMTDDIRVILIRLADRLNFMR
                     TIEKVPFEIQRKISAETINLYTPLAHRLGLYKIKSELEELAMQHVYPEIYKSIADKIE
                     HSKESQKLYIKEFLKPIERELKKTSLIYEIKSRTKSIPSIWSKMKKQNIEFEQVYDFF
                     AIRIITDSLLANEKRDCWEVYSLVTNIYKPNPSRMRDWISAPRSSGYESLHATVIGPQ
                     GKWVEVQIRSKRMDEDAEKGRAAHWKYKSASGSESGADLRLRNIRTVLENYLLVDRET
                     ERNTKIEPFDRNIYIFTPQGDLKKLPAGATALDFAFEVHSDVGNHCTGAKVNNSFVPL
                     KYILKTGDQVEIVTSKNQRPSRDWLNIAITSKALNKIRRNLKEAEFRQADIGRDILLR
                     KLNQLKITNADEAIHKLVTHFKADNSLDLFHDIAIERIDIQEIKEALFPSAKPEEIKP
                     AEKTVEKKTRKSSESVSKAIVIINDDTPLSDVKLARCCHPVMGDAIFGFVTVTEGIKI
                     HRLDCTNAHQMHSRYLYRVVKARWAENAEVSNYLATIKITGNDRVGILSSITTVLSDE
                     LKANTRSINIESRNGRFEGIITINVSGKSHLEMIIARISKVKDVTRVVRIN"
     gene            complement(40136..41074)
                     /locus_tag="PKI35_03745"
     CDS             complement(40136..41074)
                     /locus_tag="PKI35_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNX43527.1"
                     /translation="MRRITVLLLTVILMSAAFTSCQKVPITGRKQLKLLPSSIVNSMG
                     AENYTSFLKENPAVTPPTVQSAEVSVVGDRVKAAVVKYMNDNGMAKRLAGYNWEFNTV
                     NSTDVNAWCMPGGKVVVYTGILPLTKDDAGLAVVLGHEIAHAIAEHGNERMSQELAVQ
                     AAGIGLELYMQQKPEQTRQIFLAAFGVGSQLGTLAYSRQHELEADKLGLIFMAMAGYD
                     PQRALSFWQEMASSGGSKTPEFLSTHPSDEHRIAEIRKFLPEAQKYYVAGGQKGAVKS
                     TTTTPSPSNTSKSATTTPAGTGGKRTTGKVVIKPGK"
     gene            complement(41106..41378)
                     /locus_tag="PKI35_03750"
     CDS             complement(41106..41378)
                     /locus_tag="PKI35_03750"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNX43528.1"
                     /translation="MTKNKPLIRDVTETGHYYGHEIVLYNDDFNTFEYVISTLIEVCG
                     HTPEQAEQVALITHLKGKCSVKSGSRDELDPVCLELNNRNLTAKIN"
     gene            41472..42200
                     /locus_tag="PKI35_03755"
     CDS             41472..42200
                     /locus_tag="PKI35_03755"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNX43529.1"
                     /translation="MYFYGYNNYTNNFGRMVEKISYRELFSDWPLFKYKLSTRLSEPL
                     PGKEAQYKLAPVHRQFEKESGLTPDHAVESSVLLTLFPENNSVKSVVILRNEYDGVHS
                     GQVSLPGGRKESSDKSNYHTALRETKEEIGIQPDMLIYCGTLTSLYISRSNFNVFPFV
                     ATIEFPPSFVIDNREVKELIVFDLLDITPPGARMEKKFRLRDNFTFNAPGFLVGGHFM
                     WGATAMIFSEFLQVIEDLRAPPLS"
     gene            complement(42210..43874)
                     /locus_tag="PKI35_03760"
     CDS             complement(42210..43874)
                     /locus_tag="PKI35_03760"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNX43530.1"
                     /translation="MKPAKFISNLILIIMALSSFFSISACHDMKRKADLIVYNAFIYT
                     VDSAFSTTTGAVVIRNGKILECGHDDWILSRYDATVQINAKKHFVYPGFIDAHSHLYG
                     LAQMQRYADLSAAGSFTGVLKQLKLWNQAHPSGWILGRGWDQNKWPGRRFPDNKELDR
                     LFPGIPVVLTRIDGHAVLASTEAIRRSNLGAIPPGEAIYDGNRLTGIFLEGTADKIKA
                     AVPGLSEAETEDMLFSAAGRCYAAGLTSVTDAGLDRKDIIRLDSLQKAGKLSIRVNAM
                     LNPTEENFRTFLDKGIYKTPQLTVRSVKLYADGALGSRGACLKKPYSDDRSNFGVLVI
                     SKDVLRETCEKAYHAGYQVCTHAIGDSAVAFILRNYGIVLKGHNDRRWRIEHSQVVDP
                     EDLYMFGKFSIIPSIQTTHATSDMGWAETRLGNRVINAYPYKRLLAQNGWLPDGTDFP
                     IESIEPLKSFYAGVARKDINGAPAEGFQTTDALSRKEALESITIWAAKAAFEEEEKGS
                     IEKGKYGDLVILDQDIMKIPEKDILKTNVLYTIINGKVVFDSSKQK"
     gene            44078..44605
                     /locus_tag="PKI35_03765"
     CDS             44078..44605
                     /locus_tag="PKI35_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43531.1"
                     /translation="MDNKTFSLKFYLIVSMVVAAAFMRLVPHWPNFTPIAAIALFGGT
                     YIKRKELAFLIPVSALLLSDLFLGFHSTMIPVYASFLLIVSLGFVLRRMVSVKGIVTA
                     SLASSLLFFLITNFAVWYSGMMFYPHTPAGLMAAYVAGLPFFWNGLAGDLFYNGILFG
                     AYYLITNKVSVFNRA"
BASE COUNT        12536 a         9943 c        10053 g        12163 t
ORIGIN      
        1 taaattcgca agatgaagat atttgtacag ttcaaaattc agaattcatt gatatatgcg
       61 gggatacaat aatttatact atcaatgttt ttcccaaaac aaacttaagt aataaggaac
      121 gcacaacaaa tattaagatt aaagcaagaa taggagataa accagaagaa aagagcataa
      181 acatcatcca ggaggggagt ccggccataa cattgcatct tgaggcggat ctaatgatag
      241 atggagtgac tacgcattat agttcggatg cacttttata cccggatgga acagcctata
      301 taccaggttt taaatggccg gagttaggct ggggtactta ttgggatcgt tttggttttt
      361 gttattctac tacttttagt caaacaggtt ttgaacaacc ttggggatgg gaggatactc
      421 ttttaataca cctgcttacc tccaatccca ataatcttga aatcggagaa cttcgggatg
      481 gtacaattga attttctgga tattcctggg ccggactcac tggttacagg gtgggaacat
      541 tcggaggaac atttactttg aaaaggactg agtaaattgt accggtcccg gtttgccatt
      601 tcttttggaa taacttaccg cattaactcc ttgttataaa atatattgct acctttgcac
      661 cccaaaatac agcgcgccgg ttcttattat ccgggaaata aaaacagcgt aactgtttgt
      721 aaaacaacaa tatgttaaac cggctttccg gaaagccaca tcatttatca attttatgaa
      781 taacgacgta aacgtcagtg acgaactgaa aaacgaaggc gaaaccgaaa acaacgcaac
      841 cgaaaacgca gaaactgctg aagttgcttc ggaagaaaca gcagaaacca aaactgctga
      901 agttgttgaa gccgctgttg aaacagaagt tcccgtcaaa gaaaaaaagg ctgctgaaga
      961 acccgtagtt gctgctccgg ctgaattcga ctggaattcg atcggcaaga aacacgaagc
     1021 gtatccggct gctgagcgta accgtcttga agccatctac gacaagacca tgagctcgat
     1081 cggtgaccac gaagtggtgg acggccttgt tgtgggcctt accaaccgtg aagtcgtggt
     1141 taacatcggt ttcaaatccg acggtgtaat ccctgtttcg gaagttcgtt acaaccctga
     1201 cctgaaagtt ggcgacacta tcgaagtata tgttgaaaac caggaagata tgggcggcca
     1261 gttacaactg tcgcacaaaa aagcacgtat cctgcgttca tgggaacgtg taaacgaggc
     1321 tttcgaaaaa ggcgaaatta tcaatggttt cgtgaaatgt cgcaccaaag gcggtctcat
     1381 cgttgatatc ttcggtatcg aggccttcct ccccggttca cagatcgatg tgaaacctat
     1441 ccgcgattac gaccagtttg ttgaaaaggt catggaattc aagattgtga agatcaacca
     1501 ggaatacaaa aacgtggttg tttcgcacaa agcgcttatc gaagacgagc ttgaagccca
     1561 gaaagccgag atcatcagca agctcgaaaa aggacaggta cttgaaggaa ccgttaaaaa
     1621 tatcacttcc tacggcgtat ttattgacct cggcggtgtt gacgggctta tccacattac
     1681 cgacctgtcg tggggccgta ttaaccatcc cgaagagttt gttcagcttg atcagaaact
     1741 caaggtggtt atccttgatt ttgatgaaaa caaaaaacgt atcgctcttg gtctgaaaca
     1801 gttaacacct catccgtggg attcgctgaa ccccgacctc aaagtagggg ataaggtaaa
     1861 aggacgcgtt gttgtgatcg ccgattatgg cgcattcgtt gaaattgccc ccggcgttga
     1921 aggcctgatc catgtttcgg aaatgtcgtg gagccagcat ttgcgtaccg ctcaggattt
     1981 cctgaaagta ggcgacgaaa ttgaagccat catcctgact ctcgaccgtg aagaacgtaa
     2041 gatgtcgctt ggtgtgaaac agcttattcc tgatccctgg accgatattc tgaagaaata
     2101 tcccgtcagc tcgaaacata ccgctactgt ccgcaacttc accaatttcg gaatctttgt
     2161 agaactggaa gaaggcgttg acggattgat tcacatcagc gacctgtcat ggagcaagaa
     2221 aatcaagcat cctgctgagt ttaccaaggt aggggaaaag attgatgttg ttgttctcga
     2281 cgttgatgtt gaaaaccgtc gtctgagcct cggccacaag cagcttgaag aaaatccctg
     2341 ggatgtattc gaaagcatct tcaccgttgg ttcggtacat aaaggaaccg tggtaagcaa
     2401 tgccgaaaag ggagttgttg tatcgctgcc ttacggtgtt gaaggatttg cgccttcacg
     2461 ccatattcag aaagaagacg gctcactggc caagatcgac gataccctcg atttcaaggt
     2521 tatcgagttc tcgaaagaaa acaaaaagat catcctttcg cattcacgca ttcaccagga
     2581 tgccaaacag gctgaaaagg taaaagaaga aaacgaaaag gaaagcgccg aaatgagtac
     2641 caagcgtgca gtgaagaaaa tcaaggacaa ccttgaaaag accaccctgg gcgacctcga
     2701 cgctttggca aacctcaaag caagcctcga aaagaaggaa gaagaaaata aataaccgct
     2761 tcctcccaaa ccataaaaac cgggttggta acaatccggt tttttttatt ttagccatta
     2821 gaattttcgc tttctcaact gatggttagt atttaataaa taaatcgtta aaatgtacct
     2881 gggttttggg ctgcaaagat ggatttatca tcagagaccc cgtaaaatct ttacaagggt
     2941 taaaccgcca ttacctgttg aggacgctga acttcttact ggtgccgcac ctgacgttcc
     3001 ggagttgcca ggtgtaattg ctgagagaaa tattgaactg ttaaagaaag ataccagaag
     3061 tattaagacg acgaatcgta ttatcatatc acttttaact gtggcaataa cagcaacatt
     3121 aatttttctc ccgttcagaa tatatacata tttcaaaaca aatgatgcga aatctgtcca
     3181 atatgataag aaagcagaaa aacaattgag tgatgccatg taccggtatg gcatccattt
     3241 actgaaagaa ggtgatacaa caaaggctgc ggcccaattt gccgagtcat taaaatacaa
     3301 tcctgataac aggcgcgctg acagcgctct gcgcgaatgc tttaaatgat gaattttatc
     3361 catacacttg agacttaata aattaacagt taatgatccg tttctccgta accatactag
     3421 gaagcagttc tgctacgccc accagtgaaa gaaatcctac gtcgcaactt gtcaaccatc
     3481 atgaccggct tttcatgatt gactgcggcg aaggcaccca ggtcacgttg cgaaagttga
     3541 aaattcattt tcagaaggtt aaccatattt tcatcagcca ccttcatggc gatcattttt
     3601 acgggcttat cgggcttatc tcgtccatgc acctgctggg acgcgatcac gaactgcata
     3661 tttatggtcc gccggaactg gaggaactga tatattttca attgagggta tcacaaacaa
     3721 cgctcatcta cccgctggtc tttcatgcca caagaagcga cgtgccggga ataatctttg
     3781 aagattcttt ccttgaagtt tcgacctttc cgctgaacca ccggatagca acgaccgggt
     3841 tccttttcag ggaaaagccc gggttacgga aggtgatcaa agagaaagcg ctggaaataa
     3901 aaattccggc ctgggcttat gaaaagatcc ggaaagggga ggattttacc gacggggaag
     3961 ggaattttta tcctaacaat gagctgacgc ttgacccgcc gcctcccaga agttatgcct
     4021 attgttcgga taccgcctat catgaaccgg ttgttgagta catcaaaggg gctgatctcc
     4081 tgtaccacga agctactttc atgaacgacc gtgaagcgaa tgcaacggag aaatttcaca
     4141 gcacggcagc ccaggccggg aaaatagctc tacaggcagg cgttaaaaag ttgatattgg
     4201 gacatttctc agcccgttat gatgatattc agccattgct ggaagaagcc agggtgaatt
     4261 tcccggaagc atatcttgcc gaagaagggg cttgttttga aataccggta gctatctgac
     4321 ggtgatttca tcaacaaaga cccaggaatc gcctcccgcc gaaggatgcc atgccggcaa
     4381 agattttgtt ccctgaacca tgaaccttac aaaacggcct ttccccgaag gattggtaaa
     4441 atgaaaattc cgggtgacgt cttccttatg ctggtcgccg gcaacaacca gcttcccgat
     4501 attatgatac gacctgccat cgttcgaaac ctggcaggta acggaaaccg gatgaagtat
     4561 ccagcttttt gggtcatata gtgtcccgat gctgatttgt tttgcttcgg taacagtccc
     4621 aaggtcaagc gttaatgtga aatccgttgc ttcccatccc agccagtgcg ctttataatc
     4681 gttggctccc tgtactccat tggtaagcgc ggcaaggtcg ccgccgccgt atttagccga
     4741 aggcaggggg gaggctgtca cctttttccg gaaagcatgg ttgccggaaa cctgaacatc
     4801 gatgaaacgc ctggtggcat tgtaatattc ttcagggcta agcccggatt cattcagcgg
     4861 acttacctga ttagcccggc aggtgccgta aaaatcttca accaattggt tcatctgttc
     4921 tttgaggaca aaagcgccgt tttgatcggt atagaaccct cttggaccga acatgtcatt
     4981 tttcccgatt tcgatggcag catactgtat cgggaggcgg caaaccttta cgcgctgcag
     5041 cagatcggcg ctatctttca cggccgcttc tgcttcgttg aagagattgt tataaaattc
     5101 catgttggat tgcgagagaa atgtattggc atgcgccacc ggatgaccgt aaatatcaag
     5161 ccattcaccg gttttcttta tttcggaagt cagcctgtca atatattgtt gaatataagg
     5221 cgctgcaccg gcaaagtatc cgtcaacgaa atcccggatc acggaatcgg catttatatc
     5281 agggttccac agcaaccggg caatcaggta atttttcagt tccgacatct cgtgaccggt
     5341 accggtattt gtttgctgga aatgctgatg ggctccgtta cgggtcagga aactcagatt
     5401 tggctgcagc acatgcaggt tgggaaaagg agaggtggaa tgtgagaaat tcacggtata
     5461 atcccagagg taaatatttg aagcaatttt gccccagtcg gtgatgtcgt tgcaaaacga
     5521 caggcttgta gaatcttcgt ccatgggttt gctccggttc agttcaatgg tgcaaagcat
     5581 gatctggaca ttgggcaaag gcttggttat tttcggagcc tggcgtgaat attcgtatgc
     5641 aagggttgaa ataactttgt cgggaaattc ggaagcaaga cggttaacaa accgtatgac
     5701 cgggccggat ggcgatcctt cctcatttat aattttcatg catcgatcgc agtggcaata
     5761 ggagaagtta tcgttctggc tcaccgacca taccttttta tcaggctgtt ttgccatttc
     5821 cttcctgagc ttttctgctg ccagcctgta aacgtcttca ttcgaaaggc aaagctggtc
     5881 ctttatccgc ttgccgttca ttaaagagta atattcagga tgctctgcaa agtaggtttc
     5941 ccagggcacc agcttgttga aagtatgaac atagtatccg tcggcaaaca tttcatcgat
     6001 tgaattcagg cgctgccagt cgcggtataa ggtgtcattc ccgaagtttc catgaaccac
     6061 ccggaatgaa tttgctggat tttgaacctg gtacatggcc ggaatagtaa tatcctccat
     6121 gtgtgggacg tgctcaaccc cgggagcgta catgcggcat ccgatgtacc tttcaagcag
     6181 ttcaacaacc ccgtaaaccg aacccttttt tccgccgctt tcaatgaaaa tattctggtt
     6241 gcctgttgat aatatgaagc cgtcttcctt aagcgattct ttctgcctcg tccgttcgtc
     6301 cgcttcccag atagcaataa aatttgatgg ggatgctgac ttttcactga tgttcagcaa
     6361 gcatcctgac actttctgaa tgtattgctg caggagagcg gcagcagtcc tgtcggattg
     6421 cgtggcgttt gaagaaatca gaatactgac catcggtttt ccgtcccgca caatggcaac
     6481 tcctgtctgt ttttgggaat atgccagcaa tccggatatc agtaaggtga aaaacagggc
     6541 tttacgtaac ataactgttt taattttctc cgtaaaatta atcattgccc ggacacattt
     6601 tatggagccg ttgctgaata ctgtttaatc atatagcctg atgaactcaa agtcgttgcc
     6661 tgtccattgc atgaaccgcc agaaatcctt cccgtttacg atgatggatg ccgtattgat
     6721 gcacggatga aaacttatct tttcgcagtt tttcaggttc tcatcaaaga aaacgcggac
     6781 gtgatgatcc gaatcattga tcaggtcata aggcgatacc gatccgggag ctacgccgag
     6841 gtagttcatc agccgctgtt gggaggcaaa ggtgagtttg ccctgtttca accgcttttc
     6901 caggtcatga atgcccaatt cctgacgatg gtccaacact acaagatagt gccggtcgcc
     6961 tttatgattg cggaagaaga tattcttgca gtgcgtcgcc acgatatctt tccagtattg
     7021 catggctatt tcgatagtgg gggccggcgg gtgctcatga tattcaaatg ggatgcccaa
     7081 atcatgaaga atcgaataca gccttggatc tccgttcatg gtaatctctg tttataaaaa
     7141 gtgcaggttt gtacctgaat gatttcggta aaagcctgca cctgaatgtt taacataaaa
     7201 taatttacag cggacggatt ttaatattcc tgaaccatat catgcttcca tggttttgaa
     7261 ggcctattga gcctttcctg gcctgcccgt aagccggata gtctttccat tttccttcgg
     7321 tacgcttttt attccattca tcattccagg ctgtgaattc aagaattttg acaccgttca
     7381 gccagtgttc aacatgaccg ttgttaaaca cgattttaga agtgttccat tccccgacag
     7441 ggttgagttt tttaacggtt gcatcgggaa ttgtcatggc atagtcggcg ccggttttct
     7501 gccattcttc gagtttatcg ggccagccca ggtcgtcaat caactggtat tcagggctgc
     7561 attcatatgg attgccgaat gcagcatctt ccacaacatg atagaatatc ccgctgttgc
     7621 cctgaggcga tattttccag tcgaggctta attcaaagtt tgagaatttt tgtgtcgtga
     7681 tgatatcata gcccattccg ccgccttcgc ccaggcagac caggtcgcct ttctgcacgg
     7741 tccaccgcgc cccgcattcg ttgtggttat atcctttcca tccgttcagt gttttaccgt
     7801 cgaataacag ttgccatccc tgtttgatct cttttttagt aagcgtgtta gcccgggctt
     7861 ttttcacatt ttgggatgtg caaaacgtgg atgctattat cacgaggacc gtcgtgagca
     7921 gaattgacaa ttttttcatt tttttagtca ggttagatta tttatgttgt taagttttaa
     7981 cgcgtttcaa gtcagtcaat tgtgattccg attccaagga ccatcctgta gaggataatt
     8041 acaacaatcg tgatgatcac cggtctccag tgaacctcaa tttttttcgg acgttcttca
     8101 aaaaagtagg gcggatattt attaattccc cacatgagcg gcagtatgaa taatcccggg
     8161 gaaatcagca acaagaactc atacagtgat aatggcatcc tgatcaggaa aagcagtatc
     8221 gttccgatga aatacgggag ataaaactgg tatctgcgga atgccgggcg catttcaggc
     8281 gtcagcatat tgtaataggt attggctgaa tagaggaaag ggcgcgtcat cagtgaaccg
     8341 gaagccaata tcaggaacag gcagatgaac aacaggatca ttttagcagt gtcctggagg
     8401 tacatccagg agagaacata gccaaaacct tcgaaaataa aagcgcccag cataacggat
     8461 cccaggaaca tattaattcc gtgaataaat ccccaaagga aaaacagcct taataagcca
     8521 tcaagcacta gcgctttaag ataaacaatc aggcatagca ggcctataat aagggaaatg
     8581 acggctccgg ccgaatagac cactttaacc gaatcgaagc tccaggcttc aggtttcaga
     8641 aggtaatcgg ccgcattctg atgaaaaacc gaaggtatct ggaaggcata aaaggcaatt
     8701 gcggaggatg tgaactgaaa aatgaaagct ataaccaggt aggagagtat ataatacgct
     8761 gcagaattta cagtaatgcg aagcgattct ctgttcaggg atttcatccg gtattgcttt
     8821 gtacaaagct atgaaaacgt tgtatcggtt gggaatgaca ccaaaaaaaa tacccggcat
     8881 atccgggtat tttatcggta taatatcctg gtcacacttt tgagaagtca gcgatagtgt
     8941 tgctggtata cttacctgct tcaaggtttt tgcgaattac ccggaaagct tcaatggtat
     9001 aattcacatc ttccagcgaa tgtacggcag tcgggatcaa acggataagg atgacgcctc
     9061 tgggtactac cggataggtc actatggaac agaaaatatt gaagttttcg cgcaggtcca
     9121 gaatcacctg ggtggcttct tcgggcgttc cgttcaggaa aaccggcgta acaggcgatt
     9181 cggtgttccc gatattgaaa ccttcatttt tcaggcccgt ttgcatggcg ttgaccactt
     9241 tccagaggtt ttcacgaagc tgcggttgtg tacggaccag ttcgagcctt ttaagcgccc
     9301 cgattaccat aggcatcggc aacgacttgg caaaaatctg cgagcgcatg ttatgtttca
     9361 ggtaatcgat gatccgggca tttgaagcaa taaagcctcc aataccggcc attgccttgg
     9421 cgaaggtgcc gaagtaaagg tcaacgtctt tttgcacccc gaaatgttca ccggtgccgc
     9481 gaccggtggg gcccattgtt ccaaaaccat gagcgtcgtc gatcataagg cggaaatcga
     9541 actctttttt gagctcaaca atgtctttga tttttcccaa atcgcccgac atgccgtaaa
     9601 cgccttcggt gatcaccagg attccgccgc cgctttgttc cgttaaacgg gttgcgcgtt
     9661 caaggccttt gcgtaagttc tctatattgt tgtgcgggta aacaaaacgt ttgccgatgt
     9721 gaagcctcat accatcgaga atacaggcat gagcttccga atcatagacg ataacgtcgt
     9781 tcctgtcgac caggctgtcg ataacggaaa ccattccctg gtaaccgaaa ttgaaaaggt
     9841 atgccgattc tttgccttcg aattctgcca gttcgcgttc cagttgctca tgatagtcag
     9901 tttgaccgct catcatacgg gctcccatcg gataggccag tccccagcgg gcagcagctt
     9961 ctgcgtctgc tttgcgaact tcaggatggt tggccaggcc gagatagttg ttgaggctcc
    10021 ataccagtcg ctccttgcca cggaagatca tgtgactgcc gatttctcct tcaagtttcg
    10081 ggaacatgaa ataaccgtcg gcatctttac catattgacc taaagggcca agatttttaa
    10141 cgcgtttttc aaaaatatcc acagtatcag attttaatga gtttttcaaa gtggtttttc
    10201 actgcaaagc taaatgtttt cttcatagcg cacaaaatga tcacaaatcc aggggctcgt
    10261 attaaaatgt tgaaagtcac gataaactta aatttcatta aatatttaat tgtttaatat
    10321 gggaaattta accattttga ccatgtaata attccttcat ttggcacgtt tcatggtacg
    10381 cgaaaaaaat gtatttttgc aacatggtaa aaaagctcct tttatactcg atgctgattt
    10441 tcatgatcgt attatcgtca tgcagcaagt accagaaatt attgaagagt tcggataacg
    10501 atctgaaata tgacaaggcc atcgggtatt acgagaaagg cgagtatttc agggcgcagc
    10561 aacttttcga gcagatattc tcgtttttca ggggaaccga aaaagcggaa aagattgcct
    10621 atctgaattc atactgctac tataagcaaa aggatttcat catgggcggt tacaatttta
    10681 agaattttgc cgctaactat ccttccagca aatatgccga agaatgtgca tacatgagcg
    10741 cctactgcag ctacctggat tctccccggt caagccttga tcctacaagc acaaacgatg
    10801 ccatagcggc tctgcagctt ttcatcaacc agcacccgga gagtgaaagg gtggaggaat
    10861 gcaacaacct tatcgatgaa ctccgtggca aactcgaaaa gaaagactat gatatagcta
    10921 cgatgtatta caaaatggct gattttaaag ctgcaatcat cacattcaat aatgttttaa
    10981 aagattttcc cgatacaaag agaaaagaag acattttgtt tcagatactg ctggcaaaat
    11041 atgattatgc catcaacagt gttgaatcaa agaagaaaga acgccttaac gatgccctgg
    11101 aatcctataa attgttgacg tccgaatttc ccgacggcga tttcactgcc agggcccgca
    11161 gtattgaaaa gaacatcatg aagttaatgt aatcgttaat aaaacaggat aaaagcgtat
    11221 ggattacaag aagttaaaaa ttgacactac tactgttacc cgcaatgtag gcgaattata
    11281 tgcacctacc gaaaacatat atgaaacagt agccatcctg gcaaaacggg ccaaccagat
    11341 agggctcgaa ataaaagaag aactggataa taaaatcgag gaatttgcaa cacctaccga
    11401 taacctggaa gaagtgttcg aaaaccgtga gcaaatagag ttggccaagt tttatgaaca
    11461 cctgcccaaa ccaaccctta ttgctaccaa cgaattcctt aacggccaga tctttttccg
    11521 tagcccgtcc aaggatctca aatccgaata attagctttc aacgagtatg atccggggaa
    11581 aggcacaggt tttccggatt ctttattttt ttgatagcag ggtaaccttg acctctcaat
    11641 caaattttca ggtatgctga aaggaaaaaa aatcattgtt gcgattaccg gaagcattgc
    11701 tgcctataag atcccttttc tggtgcggct cctgatccgc gacggcgccc aggttaaagt
    11761 actgatgaca ccctcagcct gcgattttgt aacgccgctt actctttcaa ccctgtcgca
    11821 atctccggtt tatatcaaac ctttcaatga atcggatgga tcctggacaa gccatattga
    11881 gctgggaagc tgggccgatg ttatgctgtt tgcacctctt tcggccaata ccatggcaaa
    11941 aatggccaca ggaattaccg ataacctcct tacggctgtt tacctggctg cccgctgtcc
    12001 ggttttcttt gccccggcta tggatgtcga tatgtacaac catccgtcga ctcaggcaaa
    12061 cattaaacgg cttcaatcgt ttgggaatat ccttattaac ccccgtgaag gtgaattagc
    12121 cagcggattg agcggctgcg gcaggcttga agaacccgaa aatatcctga aggtgttgca
    12181 tcggtttttt gccaataaag ccagccttgc cggtaaaaaa gtgttggtaa caagtggtcc
    12241 caccatcgaa ccgatcgatc cagtgcggtt tatcagcaac cactcaagcg ggttaatggg
    12301 caacagcatt gccgttgaag cagcccaacg gggggccgaa gtagcgctta tatcagggcc
    12361 tgttcacgat ctgcccgtgc atccgaacat acaaaccatt aaagtatcta aagcgggaga
    12421 aatgctggaa gaaacagtca gccgtttcgg gcaggccgat atcgccatca tggcggctgc
    12481 agtctctgat tacacaccgg tgaatgtttc ggacgaaaaa ataaaaaaat cagcagggga
    12541 gggccttacc cttcagttga ctcaaacaaa agatatcctg gcagaattgg gcaaaataaa
    12601 aaaaggagga caggtgttgg taggttttgc gctggaaaca gagaatgaaa ttgaaaatgc
    12661 cgtcaggaaa ctgcatacga aaaaccttga ttgtatcgtt cttaattcaa tgaaggataa
    12721 aggagcaggc ttttcgcatg aaacaaaccg catatcaatt atcagcggga agaaccaggt
    12781 ttgtacttat gatctgaagc cgaaaaccgc tgttgcttcc gacatccttg attacattac
    12841 cgaacatttt ctgaaggtat aatttgtctg ttaataaatg attcgctcat gaaacgtagc
    12901 attttactgc ttcttgtcac tgttcttcct ttgcttatga aagcccagga atttatctgc
    12961 caggtttcgg taaatgctcc cagcgtggaa ggttcggaac gtaaggtttt ccaaaccatg
    13021 caatcggccc tttatgagtt tatcaataac cggaaatgga caaattacgt ctacaaaccg
    13081 gaagaacgga ttgagtgttc catgatgctg acaataacgg agcgtgtaac ctccgatcag
    13141 ttcaaagggc gtttgaatct ggttttaaaa cgtcctgttt ataagacatc ttacaacacc
    13201 aacctcctga attatgttga tgaagccata aacttcaagt atgttgaata tgagccactg
    13261 gtttataatg atgatgcgtt tacctcaaac ctgacgtctg tggttgcgta ttatttatat
    13321 gttttccttg gcctggatgc cgactcatat tccaatatgg gcggaacgcc ttatttcgaa
    13381 aaagcaaaag ctattgtaaa tacggcacag aatgcttcgg aaagtggatg gaaagcattt
    13441 gagagtacca aaaacaggtt ctggctggtt gaaaatctgt tgaatggcgc atattcccct
    13501 ttgcgggaag gcctgtacca atatcatcgt ctcgggctgg atgttatgtc ggataatata
    13561 gacctcggaa gatcggcggt ttcggattgc cttgacaatt ttcaacgtgc ctaccgtcag
    13621 aaaccgggat tgttcatcct gcaactcatg atggaagcca aatgcgacga actgatcaac
    13681 atcttctcac aggcagcccc catggataaa acaaaggctg tcaatattct caaagaaatt
    13741 gatccttcca acagttcaaa gtatcagaag atcatgtcaa gcgggaaata gtcccggatt
    13801 ttaatatttc gtacttttat catccgttaa atttcgatcc tgaaatgctt ttacaccttt
    13861 cggtagataa ttttatactg atccgccacc ttgatatcgg ctttgaagat ggtttctcgg
    13921 taattaccgg agagaccggc gccggaaaat ctatcctggt cggcgccctg ggattgattc
    13981 tcggtcagcg tgccgacacc atggtgcttc acgacaagga aaaaaaatgc atcgtagaag
    14041 gtgttttccg catcgaaggt tatggccttg agcccttgtt tactgaaaat gatcttgatt
    14101 ttgatccggt aacaaccatc aggcgggaaa tcaccccaca gggaaaatca cgtgcctttg
    14161 ttaacgacac acccgtgaac ctgtctatac ttaaccagtt gggcgatcgc ctggttgata
    14221 tccactccca gaatcagacg cttgaattaa atgaagcagc tttccagttg gcgcttgtgg
    14281 attcatacgt ggccaagccc ggcttgctga atgattattg cggactcttt tcgcggttta
    14341 ccaaagctca gcgggaattg aacggcctcg ttgaacggca ggaaaaggcg actgccgacc
    14401 gggaattcca gcattacctt ctggatgaac tggaaacggc ttcccttcgt gaaaacgaac
    14461 aggaagaagc cgaagcggaa ttggtcgtgc ttacgaatgc cgaggagatc aagacaaggc
    14521 tctattccgt gataaacctg ctgaacgaat ttgagcccaa tatgatagga cagctagccg
    14581 aaatccgtca gcttgttgat caggcggtac atctcggcgc cacattggaa cctgtatccg
    14641 aaaggcttga aacctgccat atcgaattaa aagatatggc cgctgaactt acggcatatg
    14701 ccagccatgt tcatttcgac cctcaacgta ttgaagaatt aaccacacgc accgacctga
    14761 tttatcacct gttacaaaag cataaagttc aatcggtcgg cgaactcctg gcaataaaag
    14821 atcagctttc gctgaacctg gaaaatattg agacgttaca gtcccagatc gatgaactga
    14881 aatcggaaat ttcacaactg gaaaagcaat taatccgggc tgcgcatgaa ttatcggaaa
    14941 gccggaaagg cgtgattccg tttatagaaa aggagatggt taaaacgctg gccgacctgg
    15001 ggatgccggc agccgtattt accatcgggc atgaagtaaa cggcgaattc acaaaatcgg
    15061 gaattgataa agtcactttc ctgttttctg ccaataaagg cgggcagccc cgcgaactcc
    15121 ggaaagttgc ttcgggcggg gaattgtcaa ggctgatgct ggcggttaaa tcactggtgg
    15181 taagccgcag catgctaccc accattctgt ttgatgaaat cgacagcggg gtttccggtg
    15241 agatagccgg acgcatcggc agcatcctgc ataaaatgtc ggcaactgtg caggtgattg
    15301 cgattaccca tcttccgcag atagccggaa agtgtaaaac gcattatctc gcttataaag
    15361 aggaaggaga aaaaagcact ttctcaagcc tgaaacaatt atcccccgaa gaccgcataa
    15421 ccgaaatcgc caggatgctg agcgatgaca tgataacgga aacagctaaa gctgcagccc
    15481 gggaattaat aaggaactga ttccttagga aatggccgga atatccggat tgattattaa
    15541 tcaagtaaac attaagatta tggcttacaa cctgctcaag ggcaagaaag ggattatttt
    15601 cggtgcgctg aacgaaatgt ccatcgcctg gaaaaccgcc gaaaaagctt atgaagaagg
    15661 cgcagaattt gttcttacca atactccgat ggccttgcgc atgggcgata cccacatcct
    15721 ggcccggaat tgcaacacaa tcgtaattcc tgccgatgct accagcattc ccgaccttga
    15781 aaacctcttc gtgaaaacaa tggaacattt tggcgggaag atcgatttta ttcttcatgc
    15841 tatcggaatg tcgcccaacg ttcggaaaaa gattccttac gaccagataa actatgattt
    15901 ttatctcaaa accctggata tttcagcgtt gtcatttcac aaggtcctgc aaacagccaa
    15961 taaattcgat gcgctgaatg aatggggctc tgtggtagcc ttatcctata tcgcggccca
    16021 acgcaccctt ttcgaataca acgatatggc cgatgccaaa gctgccctcg aaagtatagc
    16081 gcggagtttc ggatatattt atggccggaa gagccatatc cgtatcaata ctatttcgca
    16141 atcacctacg ctgacaacag caggcagcgg cgtgaaagga ataaacggtc tgatggactt
    16201 caccgaacgt atggccccgc tgggcaatgc cacggctgaa gaatgcgccg attattgcat
    16261 aaccctgttc agcgacctta ccaaaaaggt aaccatgcaa aacctatata acgacggcgg
    16321 attcagcagc atggggatga gctaccgcgc catgcaaatg tacaaccgca gcctcgattg
    16381 caaagaatgc aacgggcata agattgacga agataaggaa gaccggttat aaccggcgca
    16441 gattccggga tatcaggaat tttcaggctg gctgccgaat gtaatggtat agccatagga
    16501 agagcgtggc tgctgttccg gctttttacc gcccgaggga ctggtggccg tataattgat
    16561 cctgaacgct gtgctgatct cttccttttc tgtttgagct accggcagca tgaaagcaat
    16621 acgcgttttc atcgtcactt tcatcttcac ccctttgttt ttaacagcag ggtaggttat
    16681 gagttttatc aaacgtatag cctcttcgtc gcaaccatag ccaattccgc gggttacggc
    16741 aatattttcc accaccccga tattgtcaac agtatagtca agataaacgg ttccctggat
    16801 gttgtttttc agcgcctctt caggatagcg cagattttcc ttcacaaatt cccggaagga
    16861 gcttttcccg cccggaaatg aaggcatttt taagaattta tgcggttttt gcggtttgtc
    16921 ggaaggttgt ttcatctatc aaacagaaaa gatgttgagc aactgtaaaa ttatgcatta
    16981 tgaagctatg ttcaatggat aattaatcca tggtttaaaa gtttatcaac tggttaatca
    17041 actgattatt attttattag gtatttttca ggggactttt tcctgaagtt ttagcgccgt
    17101 tttgtcaccc gatttccctc atcaaagtcg aaaaagtgcc ctgtttggga gtttgtttta
    17161 tctttgccgt cctgaaatgg ggaaaaagaa gttatcttta ggttcaatcc ttttccgatt
    17221 aattaaagtt agaaacataa aatcctgatt cgatgaaagt tttagtctgc atcagcaatg
    17281 tacctgacac tacgactaag gtcagattcc ttgatgacaa taaaaaacta gataccaatg
    17341 gcatacaatg ggttatcaat ccctgggacg aactctccct gacccgtgcg ctcgaattga
    17401 aagacgttgc ggccaacgga atagaaaagg taacagtagc ccatgtagga actgtaactt
    17461 cggaaccgac tatccggaaa gccctggcta ttggcgccga tgatgctgtt cgggtgaatg
    17521 ccgatgctgc cgacgcttat tttgttgctg cacaattggc cgaagtgctg aagaaaaatc
    17581 cttacgatat tatcttatgc ggcattgaat caagcgacca caacggatcc acggtaggag
    17641 gaatgctcgc ggagatgctt gacatcccat cagtatcggc ggtttcctcg ctttcggtcg
    17701 aaaatggcgc tgccattctg acccgcgaaa ttgatggcgg aaaagaagtc gttacggttc
    17761 cggccccgtt tgttgccatt gtccagaaag gaatagccaa agagccccgt attgctgcaa
    17821 tgcgcggaat aatgatggcg cggacaaaac caattgcggt tactgaacct gctcctgttg
    17881 aagcattgac cgaagtaatc gggtacgaga tgcctaaacc caggactgcc tgcaaatttg
    17941 tggatgctga aaatccgaaa cagttgattg attttcttca gaacgaagcc aaaattctct
    18001 aaccacgata aaaaaaacat acgatatgtc agttcttgtt tttgttgaaa attgggatgg
    18061 taaattcaaa aaagcctcct ttgaacttat ttcatatgca gcaggtgttg ccgaaatgtg
    18121 cggcacacaa gctgttgctc tttcaatagg aaatgtagga cagcctgagc tcgataaact
    18181 taccggtttc ggcgctgcaa aaataattgc tgcaagcaat gataaactca atgttctcga
    18241 taaccaggcc tatgcttcgg taattgcaca aatagccatc aacgaaaaag cgtctgttgt
    18301 agtgctgtcg aataacaata ccgggaaagc gctcgctcca aggctttcag tcaggctaaa
    18361 agcaggaatt ggggccggtg taaaccagtt gcctctgagt gcagaccctt ttgttgttca
    18421 taagaaagct tattccggta atgcattcgc caatgtcctg attaaaacac cggtaaggat
    18481 actgacatta gcccagaatt cttttgaact gaaagaatca gccgctaaag cccccgttga
    18541 acatattgca gtagatgtgg aagccggcat ggtaaaaacc acggtgaagg atgtccaaaa
    18601 acaaaccgga aaaattcttc tcacagatgc cgaaatcgtc gtttcaggcg gcaggggaat
    18661 gaaatcgccg gataactggg ggcctcttac cgaactggca gatttacttg gcgcggcgac
    18721 agcctgctca cgtccggttt ccgatgaagg ctggcgttct cccgatgaac ataccggtca
    18781 aacaggcaaa atcatcgccc cgaacctgta catcgcagta ggcatttccg gcgctacaca
    18841 gcaccttgcc ggcgtaagcg cttcgaaata cattgtggct atcaacaccg ataaagacgc
    18901 cccgatattc ggcgccgctc aatatggcat tgtgggtgat gctgccaaag tacttcccaa
    18961 acttgttgaa gccgttaaag aaatgaaagc caaatagcgc ttttcgcggc tgtgatacaa
    19021 ccgaaatctt cataaaccgg gctgattctt catgcctggg aagaatgtgt atgctgattc
    19081 atgattaatc attgagactc atttaactta tcagttatgg taaaacaaat tgtctttgca
    19141 attgcgctta taattaccct gctggttttt gcctatacca tcagccggat tattggtttc
    19201 ttcaggctaa ccaggaaagg gtttcctgtt aaagagatag gtaagcggtt tggaataatg
    19261 atgaaagtgg ctttcggcca gaaaaaaata ttccgccgtc ctgttatcgg attattgcac
    19321 gcactggttt tctggggctt ttgtgtaata ttgttcggga gcatcgaaat ggttatcgac
    19381 ggtctgtcgg gagccaggaa atcgctgtcg gtgctgggtt ggtttcatga ccttatcatg
    19441 ctatcgggag atatttttgc gctgctggta ggcatttcca ttatcgtttt cctgataagg
    19501 aggcttttcc tgcatgtcag gcgtttcgaa ggtatcgaaa tgaaaaagaa atcgcacacg
    19561 gatgcaacga tagccctcac gatgatatta ttactgatgg tttccttgct gggaatgaat
    19621 gcctcttatg tggaatcgtg ccggcagtcg ggcgaaacct tgtatggcat ttatccggtg
    19681 agcaatctcc ttacaggtgc gttttcgggc atgcaactga aaaatgttaa tatctggaat
    19741 gagattttct ggtggagcca tattttgctc atttttatct ttgccaacat attgccgtat
    19801 tcaaagcatt tccatgtttt catgtcaatc ccgaacgtgt tcttgtcaag gcttgatccg
    19861 ttgggcaaat tgccgaatat ggatagtatc acccatgaag tgaaattaat gatgaatcct
    19921 gaaactgctt atgcagcgcc tgcttctgat accccggtcg aacgtttcgg ggtaaaggat
    19981 gccgaggata tcaactggaa aaattatttc gattccctgt catgtaccga atgcggccgc
    20041 tgcacctcgg tttgtccggc aaatattacc ggtaaaagac tttcaccccg caaggtcatg
    20101 atggatgttc gagccagaat gaaggaaaaa ggtccgcatt tattaaagga caaaggatat
    20161 gatgactccc ggtctcttat ccgcgattat atcacggagg aagaaatctg ggcttgcact
    20221 acctgtaatg catgcgctat ggaatgtccc atcaatatca accatccgac actgattgtg
    20281 gatatgcgcc gctatcttgt tatggaagaa gcatctgccc ctggtgaact gaagaccgtt
    20341 ttcaacaaca tcgaaaacaa tggcgccccc tggcagtttt cgccggaaga ccgtttgctg
    20401 tgggccgaaa acattgaaat gaacatgaac taacctttga aaagacattg ctatggaaaa
    20461 agttaaagtg gaagtccccg ttatggctga tctctttgca cggggagaaa aaccggaata
    20521 ccttttctgg gttggatgcg ccggcgcttt tgacgaccgg tataaaaagg ttgcagccgc
    20581 tttcgttaaa attctcaccc atctgaaggt gagctatgcc gtattgggaa aagaagagtc
    20641 ctgctccggc gacccggccc gccgggccgg caacgaaatg ctgtaccaga tgcaggcgct
    20701 gcaaaatatt gaagtgttca aggcctataa tgttcagaaa atcattacaa tttgcccgca
    20761 ctgttacaac attttcaaga atgaatatcc tgacctgggc ggtcattata cggtgataaa
    20821 ctacctgcag tttatacagg aaaacatagc cggcggaaaa cttattcttc cccaaaatgc
    20881 ctttgactca aaagtcatca cctatcatga tccatgttac ctgggacgtg ccaacggcat
    20941 ttatgaagag ccacgggcaa ttttggaaca ccttacgcgc aactttgtgg aaatgggccg
    21001 caatcgcagc tttgcccttt gttgcggcgc tggcggggga caaatgttca aggaagctga
    21061 aaagggtaat aaggagattt ttatcgaacg taccgaagat gccctgaata caggttgtaa
    21121 tatcattgcc acagcctgtc ctttttgtat gacaatgatg accgacggca tcaagtataa
    21181 aaaccatgag gaggatgtca ggaattacga tattgccgag ctcatcgtac aggcattgaa
    21241 cctgtagttc cggtcggaca atttcagaca taatgaacag aaaaaaaata aaatagctac
    21301 cgaaaaaaaa tacttgccat ggaaaacaga aaagtgttaa aaagcggcga attcctcgta
    21361 gcagaagttg aacccaacga tgtgttcatc cccgaagaat tcaacgagga acagttgatg
    21421 atcgcgcaaa cctgccgcga ttttcttgtt accgaagtat atccaaacct cgataaactt
    21481 gacaaacaag accgtgaact catgaaggaa atcctgaaga aatcaggaga actgggtctc
    21541 atgggcattt cgttgcccga agaatacaat ggcttcggac aatcatttgt gactcaaatg
    21601 ctgacagccg aaacaatcgg ggccggttac tcattctcgg tcgccttcat ggcacattgt
    21661 ggtattggta ctttgcccat catgtattac ggcaacgaag agcaacgcca gaagtatgtt
    21721 tccaggcttg ccacgggcga attgattggc tgctattgcc ttaccgaacc cggtgccggc
    21781 agtgatgcca attcaggaaa gacaaacgca aaactttcgg aagatgggtc tcattatatc
    21841 ctgaacgggc aaaaaatgtg gatcaccaat gccggttttg ccgatacgca ggtcgttttt
    21901 gccaaaatag agaacgatcg cgtgctcagc gcctttgttg tcgaaaggac aatgccgggt
    21961 gtggttgtgg cccccgatga aaagaaaatg ggaataaaag gttcgtccac tgcccagatt
    22021 ttctacaatg atgtgaaagt gccggccgaa aacctgattg gaaagagggg ggaaggcttc
    22081 cgtattgcgc tcagcatact ccatatggga cgcctgaaac tcggcgccaa tgttataggc
    22141 gctgctaaaa aagctataac ggattcggtg aaatatgcca acgaaagaaa acaatttggc
    22201 gtactgatct ctactttcgg atccctcaag tataagatgg cggaacaggt tattaaaacc
    22261 tttggccatg aatcggcaac ctaccgtgcc agctacgata tcgatcacct gatcggtgaa
    22321 ctgaaagcca ccggcatgga caaaggccgc gcaaccatcg aagccatcag ccattatgca
    22381 gtggaagcag ccctgctcaa ggtatatgga tccgaaatgc tggactatgt tgccgatgaa
    22441 gccgttcaga tccacggagg tatgggatat tctgccgaaa tggcagttga acgcggctat
    22501 cgcgattcac gtatcaacag gatttttgaa gggactaacg aaattaaccg gcttctggta
    22561 gtcgacactg caatgaaacg cgccatgaaa ggcgatttcg accttttcgg cgaagccgaa
    22621 aaactggctg ccgacctggg ttctgttgct tgcaagattt tagggaacga aacttactat
    22681 gaagaaaaat cggtgtacct gaaaaatttc aagaaagtag cccttcttgc tatcaatggc
    22741 gcttcaaagc atttcgggaa acagattgcc caggaagaag aagtcctgaa caatatttcc
    22801 gatatcatga tgcagatcta cgtggcggaa tccatgtccc ttagaatcca aaagcttgag
    22861 gcagcccgtg gtgcagatgc cgttagtgta taccgggata ttcttgatgt ttttgtatac
    22921 gattcggctg acctggtgcg caaatcggcc atagatgccg tcaatgcctt tgcaactgct
    22981 gaattgctgc ctgcactgca ttctgccgta gattcactta ccagggtagc agctttaaac
    23041 gttaaagaat cacgccgccg tattgccgac aaacttattg ccgacaacga atataagttc
    23101 tgatccggaa ccaattaaaa agaaacagcc tggttcttta ggaatcaggc tgttttattt
    23161 ttggaggcgt ccctggttac ccggtaacag attctccggt taccggaggt atctcaagtt
    23221 ttgccgacgg caacactttg atggagagtt cgccggcatc tttttcatga tcaatcagga
    23281 tggtatcgcc ttctttaagg gttgatttaa tgatttcctc agccagcgta tcttcgatgt
    23341 atttctggat ggccctttta agcgggcggg cgccgaattg ttcatcccag ccccggtcaa
    23401 ggatgaaatc cttggcggct tccgtaatct gaggctggta acccattttg cggattcgat
    23461 caaacagttg acgcaattca atatcgatga tcttatgaat atcatcacgc cccagcgatt
    23521 caaaaatgat aatgtcgtca atcctgttca gaaattcagg tgcgaaagtt tttttcaggg
    23581 cattttcaat aactccctgg gcctggtttg accattgcga ttgcttggca acagttgaga
    23641 acccgacgcc ggtaccgaaa tccttcaatt gccgggatcc gatattggaa gtcatgataa
    23701 cgatggtatt tttgaaatcg accttgcggc caaggctgtc ggtgagaaca ccgtcgtcca
    23761 ggacctgcaa caggatatga aagatatcag gatgggcttt ctcaatttca tcaagcagga
    23821 caatagagta ggggcggcga cggacttttt cagtcaactg gcctccttct tcgtagccta
    23881 cataacccgg aggcgccccg accagcctcg aaacggcgaa tttttccata tactcgctca
    23941 tatcaatcct tacaagggtt tcttcggaat cgaagagata ttttgccagg accttggcaa
    24001 ggtgtgtttt tccgacgccg gtgggcccca gaaaaataaa ggtcccgata ggcttgttcg
    24061 ggtctttcag gccggcacgg ttacggcgga tagctttaac tacttttttg atggcttcgt
    24121 cctgcccgat tacagcgcca cccagttcat tttccatgtt cagcaagcgg tcgctttcat
    24181 tctgggcgat acgctgaaca ggaacgccgg tcatcatggc gaccacttcg gcgacattct
    24241 cagccgaaac tgtttcgcgg ttgatctttg actcatcctc ccatcgccgt ttggcgcttt
    24301 ccaggtcatt ttgcagacgg cgttccgtat cacggtaatt ggcagcttct tcaaacttct
    24361 gagccttgat ggctttcatt ttccgctgct taacttcttc tatctgagct tcgatgttca
    24421 caatttcaac cggaacatgc atgtttgaaa tatgaaccct tgcgcctgct tcgtcgagtg
    24481 catcaatggc cttatccgga aggcaacggt cggtgacgta tcggtttgta aggaaaacgc
    24541 aggcatctat cgcttcatcg gtatatttga ccagatgatg gtcttcgtat ttctctttga
    24601 tctggttgag aatctctatg gtttcctccg gactggtcgg gtcaaccagc actttttgga
    24661 agcggcgttc cagcgcgccg tctttttcaa tgtactgcct gtattcatca agggttgttg
    24721 ccccgatgca ctggatttca ccccgggcca gggccggttt gaacatattg gatgcatcca
    24781 gcgaacctga agcattgcca gccccgacaa tggtgtgaat ctcatcgata aaaagaataa
    24841 tatcccggtt cttttccagt tcattcagca ccgctttcat tcgttcttca aactgcccgc
    24901 ggtattttgt gccggcaaca agcgatgcca ggtccagcgt aaaaaccctt ttattgaaca
    24961 aagcccggga aactctccgc tgcgtaatcc tcaaagctaa tccttcagca attgccgatt
    25021 taccgacgcc cggttcaccg ataagtatcg ggttattctt cttacggcgg ctcaggattt
    25081 gagaaatgcg ttcaagttct ttctcccgtc cgacaatagg atcgaggcgg ttttcttcgg
    25141 cagcgcgggt cagatcacgt ccgaaattat ccagaacagg cgttttcgac ttgctgtcag
    25201 caattttttt gctcccgcca aatggcgatc cctcatcagt atcgtcgtcc tgcgctccgc
    25261 cgggcaactc atcgtgaaag tcggtccggc tgtctttttc aggtctgtca acgccgcggg
    25321 aagcgtcttc atccagcgaa acaaggtacg acttgacctc ttcttttacg atatcataat
    25381 caacgccata tttcagaact gaccgggtaa caatattatc ctcatctttt aagatggcca
    25441 gcaggagatg ctcggtacca atcaggtcgc tgttaaaaag ctttgcttca aggtacgtca
    25501 gctttaatgc cctttctgcc tgcttaacca gcggcaggtt tttaagttct tcgatgtcaa
    25561 ttttttttga ccggttcgcg attgtcgatt caatggtttt tctgatttca gcgagatcga
    25621 cattcaggta attcaatatc ttgattgcca gtccctcacc ttcccgtaat ataccaagga
    25681 aaagatgttc aacaccaata taatcgttgc ccagccgcaa agcttcctcc cgactgaatg
    25741 acaaaacgtc ttttaccctt tgtgaaaatt tagcttccat tccattctct gttactgatt
    25801 tactccggcg gcaaccatat attactgtaa ttcaaaatta acactaaaac aggcagatat
    25861 gtttgcagct tcaaaattac tttcaaaatc tgtaacgatc aattaatatg ccattgtctt
    25921 tttaacatga acctgttagt aatatgacac aaataggccg ggggcaaaga tattgcgtat
    25981 cataaaaatg attaacttcg tacaattgtt cacatgaaca taagttatta ataattaggt
    26041 agttacaaaa tggattacaa cgacgaaggc gaaaaaattg ttcaggtaaa tattgagaat
    26101 cagatgaaat cggcctacat tgattattca atgtcggtca ttgtgtcgcg tgctttaccc
    26161 gacgttcgcg acggtctaaa acctgttcac cgcagggttc tttttggaat gttcgatctg
    26221 ggtatactat cgaaccggtc atataaaaaa tctgcccgta ttgtcgggga agttttaggg
    26281 aagtaccatc cgcacggaga ctcttcagtt tatgacgcca tggttcgtat ggcccaggat
    26341 tggtctctgc gttacccact tgttgacggg cagggtaact ttggttcgat tgacggagat
    26401 agcccggctg ccatgaggta caccgaagca aggctcaaga ggcttgccga ggaattactg
    26461 gctgatcttg ataaggaaac cgtggatttc aagttaaact ttgacgattc actggaagaa
    26521 cccaccgtac ttccgtcaaa agtgcccaac ctgctcatca acggttcatc cggtattgca
    26581 gttggtatgg caacgaatat tgcgcctcat aatcttggac aggtagtaga tggtacaatt
    26641 gcctatatcg acaaccatga cattacgatt gaagagctta tgcaatatgt aaaagccccc
    26701 gattttccga ccggaggcgt tatacatgga tatgacgggg tgcgcgaagc atttgaaacc
    26761 ggcagggggc gtatcattat ccgtggtgaa acgaccatcg aaaccgatga taaaggtcac
    26821 gaatgcattg tggctacttc cattccttac cagataaaca aagccgagat gatcaagaaa
    26881 acggcagatc tggtcaatga gaaaaaaatt gaagggatca gcgacattcg tgatgaaagc
    26941 gatcgcaacg gtttacgcat cgtctatgaa ctcaaacgtg atgcaatcac caatatcgtt
    27001 ctcaataaac tgtatcagta tacctcgctt caaagttcat tctcggtcaa taatattgca
    27061 ctggtgaaag gccggcccgt tcagttgaac ctgaaacagc tcatctcaat gtttgttgat
    27121 caccgtcacg aagttatcgt acggcgtact caatttgaac ttcgggaagc tgaaaaacgg
    27181 gctcatattc tcgaaggact ccttatagcc ctggaccatc tggatgaagt aattgccctt
    27241 atccgggctt ctgtaactcc ggatgaggct aaaacaggcc tgatggagcg attcggatta
    27301 tcggaaatcc aggcaaaggc tattctcgat atgaggcttc aacgccttac gggacttgaa
    27361 cgggataaaa tcaaggaaga gtatgccgaa cttataaaac tgatacagca cctgaaagac
    27421 atactggccg acgaatcgct tcgcatgcag atcattaagg atgagctgct cgaaattaag
    27481 gaaaagtatg gtgacgaacc ccgcagccgc attgagtaca cagccaaaga cttcaggatc
    27541 gaagacacca tcgctgatga aagtgtggtg atcactatct ctcacatggg atacatcaag
    27601 cgtacgatgt taaccgaata caagcggcag aacagaggtg ggaggggagc ccgcggttcc
    27661 gatatccggg aagaggattt tcttgagcac ctttttgtag ctaccgctca caattatctt
    27721 ctcttcttta ctgaaaaagg caaatgcttc tggctaaggg ccttcgaaat tcctgaagga
    27781 ggaaaagcat ccaagggacg tgccattcag aatatagtaa acatagaacc tgatgataaa
    27841 atcagggcta tcatcaacct gaaagacctc aatgatgatg aatatgttaa taacaacttc
    27901 ataattcttt gcaccaaaca aggcaccatc aagaaaacat ctctcgaggc ttattccagg
    27961 ccccgcgcca atggcataaa cgctatcaac attgttgatg atgactgtct gctggaagcc
    28021 aggctcactg acggcaattg cgaaatcatg atggcgctgc gatcggggcg ggctatcagg
    28081 tttaatgaag ccacagttcg cccaatgggc cggaatgcca cgggtgtaag agggataagg
    28141 cttgccggtg accatgatga agtaattggc atgatatgtg gtaaagatga aaacgttgat
    28201 atcctggttg tatccgaaaa cggctttggc aaacggtcat cactcgctga ttaccgggtg
    28261 accaacagag gcggaaaagg ggtaaaaacc attaatatta ctgaaaaaac cggccagctt
    28321 attgccataa aaaatgttac cgacaatgat gacctgatga ttatcaaccg ttcaggaatc
    28381 attatccgga tggccgtcag cgacctgagg gttgtgggaa gggccacgca aggcgtaagg
    28441 cttatcaacc tgaaagaaaa cgatgccatt gcggctgttg ccaaagtcga atgcgaggaa
    28501 gatgataacc tggtcggcca gaatgaggaa gaacctggtg atgataatac atacgagtta
    28561 accgatattc ccgatgagga aaatgacgaa ccttccgaaa aagaataaaa aaatgaattg
    28621 taactaattc aaactgcata catttgccga ctattaacga cttaataaag gatagcataa
    28681 aaataaaccc aaacaccatt tataaaatga aaaaatccat tctgctgctg gccttagtta
    28741 ttatgaccag tatgactttt gcacaattta aaaaagaccg gacctctgcc tacaattact
    28801 ggcaaaaggg agatcttacg aaagccaggg attatatcga caaagccatg gcatatccgg
    28861 aagcagctac agatgctaaa gcgcattatt acaatggtgc catttatctt gatattcatt
    28921 tcaaaccgga aatgaaagaa accgttccca acgatcttca gatcgcctat gatgaaatga
    28981 agaaagcgca ggaacttgat accaaaggcg attttaaatc ggatataatg attcgcatgc
    29041 taacgcttgg cggtcaattc ttcaatgccg gcgctgattt gttcaaggct gagaaatttg
    29101 ctgaagccct gagcaatttc aaccaggctg tgaaaatcgc cagtgataac cagagccttg
    29161 atacattagc catttatggt acagctttgt gctatgagaa actaaaagac aatgagaacg
    29221 ccacggcaac ttacgaaaag ctcatcagcc taagcatgaa agaacccagc atatatacct
    29281 cgctggcttc tatttacata gatatgaaaa tgatggataa ggcagataag gttttgcagg
    29341 atggattaag gcttttcccc ggaaattatg acctcatcat ttcgatggca aatctttaca
    29401 taaaaagcaa tcagcatcag aaagcgctgg aaagcctgac aattgccaag gaaaaggatc
    29461 caacaaacaa gactattgct tttgcattgg gcgttaccta tgatttactg gctaaagaca
    29521 cgaacctgac tgttgccgat cgtaaaattt atttcgacga tgcagtaaaa gcctatgaag
    29581 aaacgataaa actggatccc aatttctttg acgcacagtt caacctgggg gttgtgtatt
    29641 acaataaggg tggcgatgtg atcaatgaag cgaataaact tcctttgaac gatccgaact
    29701 atgagaatct ggtaaatcag gggaaagaat atcttaataa agcgctgcct tacctggaag
    29761 gtgcagaaaa aatgaatcct ggcgaccggg atacgctgat ctcactcaaa gaaatctata
    29821 ccaggctggg tatgtatgat aaattgaaaa ccgtcaatga taaactaaat caataatatt
    29881 cagaaacagg ggcctgattt atcaggccat tgtttcaata tagtaattta acataatatt
    29941 aattataaga aatttgatct tgccatttga tgaatccctg aatcattgat tttgtgtatc
    30001 tttaaacaaa aaatatgaaa gtctttaaaa tcattctctg ggcgcttcta atttttatac
    30061 tggcagtatt tttgaccgga ttttttcttc cgcgtacagt ctccgtttcc cggtcagtca
    30121 tgatcaaagg ctctgttgat gacatttttg aacaggttaa taccttgaag aattgggaaa
    30181 aatggtcgcc ctggcatcac ctagacacca ccatgactat cctttattcc ggccccggga
    30241 aaggcattgg tgcaagttat agctggaaaa gcgggaataa aaatgtcggg accggtaaaa
    30301 tttcaattat caacagcttt ccatacgatt ctgtaatatt ggatatggat tttatggaga
    30361 atggcaaatc cgtgggcaaa tttattttca gcaaagcgga tacctctcat gtttttgtga
    30421 cctggactat gaccagcgat ctcggaaaaa atccggtcat ccgatatttt ggattgctca
    30481 tggaccggtt cgttggacct gattttgaaa aaggactgca aaatatccgt catgtagttg
    30541 aatcacatcc tgaaaccacc aagcctttca aaattaccga gaccgatatt cccgcccgta
    30601 tcatgctgac gattcgtgat acatgcagcg ggaaaaacat tcgccccaaa atgggcttta
    30661 tatatggaga aatattcaat ataataaaac aaaagcattt gcaggttacc ggatttcctg
    30721 ttgccatctt tcataccatt tcaccggcct tatttgatat tgaagccgga gttgcggtaa
    30781 acaaaatcgt cagtattgaa aaaaacaacg ttcagtttac agaaatgcca cagcagcatg
    30841 ccgtaatggc gcaatatttc ggcccttatg aaggaacggc tcaggcatat gctgaaattg
    30901 aaaaatacat cacggcaaat aaattatcgg ttacggctcc ggtcattgaa gagtatatat
    30961 ctgacccgaa aactttaccc gatacagcaa aatggcagac aaatatctat ttgcctgtta
    31021 aatagccggg gcgtaacatg gcttctcccc tgcctgcagg aatgatgaaa gaaatagaat
    31081 gtccgtccaa aacccgaaaa ctgatcagga cgaaccaatt ctcattcagt tattatttca
    31141 tacgtttgtt aaaccaatta cctgaaaatg gcagcaaaag ttgtccgggt accccgcctt
    31201 gttgaggttg atccctggct cattccaaac acaaacgatg taatcagcag aattgaccgt
    31261 ttccggtcac gtctggcatc cattgaaacc gatttcggca gcctgttgaa atttgcttct
    31321 gcccatgact actatggttt ccacttcgat aagcaaaata agcgatttgt gtaccgcgaa
    31381 tgggcgccgg cagcacatca gttgtacctc acaggtgatt tcaaccattg gcatggcaca
    31441 tctcacccaa tgtggcgtaa tgattatggc gtctgggaaa tttttctcga tttcgaaacc
    31501 tataaagaca ggtttaaaca cggcagcttc gttaaagtag tcgtcctgag tgatttagga
    31561 ttgcagaaac gcattccggc atacatccga agagtggttc aggatgaggt caccaaggat
    31621 tatacgggcc agctatggtt ccccagcaaa ttcgactgga agaatgacaa tttttctata
    31681 aaaaacggga aggacttgtt tatatatgaa gcccacattg gaatggccca ggaaaaagaa
    31741 ggcatcggga cataccagga attcactgat aatattcttc cccgcatcaa aaaagccgga
    31801 tataacgcag ttcagctcat ggcaattcag gaacatccct attacgggtc gttcggttat
    31861 catgtgtcta atttttttgc cccatcatcc cgtttcggaa cgcccgaaga actcaaactc
    31921 ctgatcagaa cagcgcatag tttggagatt gcggttatca tggatattgt tcactcgcat
    31981 acggttaaaa acatcaacga agggcttaat ttgttcgatg gaagtgaaaa ccaatatttt
    32041 catccgggag cccgcggtga acatccgacc tgggattcca aactttttga ttacggtaag
    32101 acggaagtgt taagattcct gctctcgaat gtaaaatact ggcttcagga atttcatttc
    32161 gacggcttcc ggtttgatgg cgtcggatcc atgctctatt ttcatcatgg tgatgaaacg
    32221 atcgacagcc cggataaata tttcaggcaa ggcgttgaat atgacgctgt tacctatctg
    32281 caattggcca accacctggc ccataaagta aaaccggatg ctgtaacgat agcggaagat
    32341 gtcacaggaa tgcccggcct gacagcgccc gttcccgatg ggggaattgg atttaattac
    32401 aggctcggca tgggcattcc tgacttctgg atcaaatacc tcaaagatct gcgtgatgaa
    32461 gactggaata tttttgagat gtggaatgta ctcaacgaca ggctgcctta tgttaagacg
    32521 gtggcttact gcgaatcgca cgaccaggca cttgttggag acaaaacgct ggctttcagg
    32581 cttatggata aagagatgta cttttccatg cggaactcag accataatcc tgtcattgac
    32641 cgggggatcg ctctccacaa gatgatccgg cttttctcaa tttcattggg aggacaagcc
    32701 tggttaaatt ttatgggaaa cgaatatggc catcctgaat ggattgattt cccccggcag
    32761 ggcaacggat ggagttataa atatgcccgc cggcaatggt cactggttga tgacaaaacg
    32821 ctgaaatacc attttctggc tgattttgac agggagatga taaaaattat ggctgaatat
    32881 agaattatgt cctccggcta tgcccgtcaa ctcaatatgg atgaagcgaa taaatgtatc
    32941 atttttgaac gtggcggatt actgttttta ttcaatttcc atcatcagcg gtcaatatcc
    33001 gattattcat ttataatccc gggagatggc aggtttcaga ttctgttaaa cagcgacagt
    33061 aaattatttg gcgggcacgg acgcattgat gatcatacgg ttcataatgc cttatttgac
    33121 aaggaaaaca actgttacag acttcaggta tatttacctt gccggtcggc cctgatttta
    33181 caaaaaacgg ttgtttaaat tatcctgaaa cattttaatt gaattaacaa atattattat
    33241 tttttatcaa aaatactttt gtataattgc cccaaaaatt tattaaagag aaatgagtaa
    33301 taagccagcg aaaaaacggg taattgtcag ttaccagaat ctctcaagcg aattaaagga
    33361 agctgttctt aagaaatacc cattgggttg ggttaacaac atgattaagg ttaaaggcgc
    33421 cggtgatacc tttttccacg ctattacgct tgatacggat gagatcagtt atcttatcaa
    33481 agttccggta agaattgatc agaaatcaga taaagatgac gaggactttt ttgcagaatc
    33541 gattgatatt aaggagtctg atgaatcgtc gggcggcgaa ggcaaagaag acgacaatga
    33601 acaagaccgg gaggaataga tgttgctttg ttcaattaac aacttttaac aataaaaagc
    33661 gaagtaacga attaattcat tgtattttag ctgcgatgtt cacagcgatg acattcccta
    33721 aggagtttat taattaaaaa acagccataa aaggagcctt tcaggttcct ttttttttaa
    33781 aaccatatca gccgaccagc agggatatct cggaataccg gtaattggaa tacgatactt
    33841 ttcgcaggag cgccatggct aatgtaagcg taccgatacg tccgacatac atcgtaaaga
    33901 ttaatatcgt tttgccggca tcagataaaa atggcgtaat ccccgttgac aaacctaccg
    33961 taccgaaagc cgacacttct tcaaatatca ggtttagaaa agggtggttc ccatcggtta
    34021 ttgacaggaa aaaggttgag ataaagatca gggaaactga aaacaaggca attgaatatg
    34081 ctttatcaat aagttcaaac gaaaccgtat gatgcatcag ctctacgttc tttttccccc
    34141 tgatggtggc atacgccgat gaaatcaggg tcgcaaatgt tgttgtttta attccgccac
    34201 ccgtggatgc aggagatgcc cctataaaca tcaggaaaat catgaacatc agcaccggct
    34261 ggctcaggtg tgagaaatca acggaattga aacctgctgt ccggcaggtc accgattgaa
    34321 acacagaagt gacgatagca ccataatacc cgtaatcctc aatggtacga tgcctttcca
    34381 caaaatagaa cacgacggcg cctgcaagta ttaatccaaa cgatgtaaag agcgccacct
    34441 tcgaatgaac gcggagtttt ttccatttca tttttcgccg ctcacgaatg cgggcaatac
    34501 taaacatatc ctcaagcacc atgatcccta tcccgccaag gaaaatcaga cccgctatga
    34561 tgagttgcag attataggca tgtcgcactc cgacatcata cagattatcg gtaaaaagag
    34621 taaatcctgc attattgtat gccgatacag aatgaaacag agagaaaaat gccttgcctc
    34681 cttttccctg gaaactgatt tcacccggcc ataaaaaata aatcagtgtt gctccaacca
    34741 attcgatgaa gaggctataa aaaataactt tctgcaatac cgttctagtt tcactcaact
    34801 ggtcggtcga caccaggtct ttgataaggg actgataacg taaccccgcg gagctccttg
    34861 agaaggtggc aaaaaaagca gcaaatgaaa taatattgat acctcctaat tgtatcagca
    34921 gcatgataat tgcctggcct ttgatggtga aacatgttgc ggtatcaaca acaacaagcc
    34981 cggtaacaca acaggcgctt gtagaagtaa agagggcgtc aataaaccgg attctgtcta
    35041 cagtcatttc gggcaagcaa aggagcccgg caccggtgag gatcaggata atgaaggata
    35101 acgccagcat ggctgatggg cccattttta tcaatgaaat atgctcactg gccctcccga
    35161 cttcaaaggc tatgaaaacg aagaagtaca gttgaataaa aacaatgaaa tattcggtaa
    35221 acttcggcag gcccaataat ttaaaaaaca taaggacaat gtggtcgcca aacaacatca
    35281 gccctattcc atccaatagc aaccccaaca tgataatagc ttcgaaaagg ttgttcttga
    35341 taaattctat cgggtgaaag tcataaaata tttttgtcag gaactttaca aaataaaata
    35401 taaagctgcc ttccatgata atgtaattga gcctgtatgt agccggcgtt ttcggaaaac
    35461 cataatagta gatgatagat ccgacagcaa ctatggctac aaaaatgctg agtatctgca
    35521 agctcctgat tacgtatacc ttatgtttgt ataaccggat attgatatac tccctgatcc
    35581 gatcaaccct agtcaatggc atattgaacc cggaatatta agcattgatt tgaaggaaac
    35641 ggtcgatatc cttaatatgg ccaaagataa gcaggcggtc ggtgacttca aggatctgct
    35701 ttgtttcagg caccccaata atatgatgtt cagccagttc ttttcctcct ttaacggtat
    35761 ggatttcctt ttttaaagta atgaggctga gtttatattt gtcgcgaagg tctatttcac
    35821 cgaaggtttt gttgatacaa ttcttcggag caataatttc ggcgatccgg taatcatcgg
    35881 gcaactgaag atagctgaca atactggggt tagaaagcct ttcggcaaca ataaccccga
    35941 cctcttcttc gggtgagagt atttcggtga tacccatttt ttcaaggata atccgttgag
    36001 cttcaccatt cgcccgggta ataagtcgtt tcacctgcaa atcgagcagg acggaggaac
    36061 aaaggagcct ctcttcaaaa tcggagccaa tggcgactac aacagcatca aattcagaga
    36121 tattttgtga ttgcagggct tttttatccg tcgcatcgag agctactgca taggctacat
    36181 cctcccctat tgaatcgatc acttcctgat tattgtcgat tgccattact tcgtatcctt
    36241 tggctgacaa gtttttggcg atagccttac caaactgacc aatcccaatt acagcgtatt
    36301 ttttggttgc catgtgtctg acagtagtta attataaaaa cctgcaaatt tacagctatt
    36361 taaatatgcc tggcatctct gatacgagta tattttttta cagatttata aatccggaat
    36421 tttcgtataa atggatcata tttggatgca gggggaattt ttatcggcag ggaaaagaga
    36481 aaaaaagatg aggaacccct ctttctgaaa ggttcctcac ctccgtgtca gcaaccgtaa
    36541 ttgctgaagc gggccgaact ccggatgcca gggattgaat aaggacttac gtcctttttt
    36601 catctgatca aaccgtcaaa cttacgtctt caagcctgat ccccggtttc ttatgcctgg
    36661 ttttcaaaaa tccttttcag aattttatca gcaaggacat aaacggcctc tttccttact
    36721 ttttgcaaag gctggcagtg ttgccttttg aaaccgtccc cggcggcagg aagattttcc
    36781 gcggttcctt cccgtaagaa gtcccgtttt cagaactggc aatgcatgcg ctgttttgcc
    36841 ttgttccggc tctttcctgt ttcatcctgg ttgtcctttc ggacaattcg ggtttcattc
    36901 agttgcggct tccgtttgtt ccctttttca aagtcccaga tttcagccac tgagccgatc
    36961 ttcctgttgg aggggatcag aataacactc ggaagtgctc ttcttttccc tgcaaaaaat
    37021 caaacacttg catgcccgtt ttttgctccc ggcctcctgc ttccctcttc ccggcttttc
    37081 ctttcggaat caccgatgtt gattccagca gtttttcgct tcgtctgtct ccctttcggg
    37141 atcccggcgt ttcgattttc ggtcaggctc catgatcgtg tccggtgaat taaaaatacg
    37201 acgcaacagg taaccaggtc aagtgttttt atggaaagaa ttaaacatta tattaacgtt
    37261 agttatgaac aattgttgaa aagtttaaaa tcgtaaataa ctgatatata ttgaattaga
    37321 tataaccatt atttttcgtt taaaatgtta agatccggta tgcctctctt aattcgttta
    37381 acagcgcaaa ttatttcttt tttattttgg aggaaggccg tttcagggac aactatttct
    37441 aagttattcc caaagcaatc cgatgtttta agcttaatgt ccttattttg agaagataaa
    37501 atacctgtta tcctgaaggt gtacttctcc cctatcctgt atacggggtg atcgggttcc
    37561 agaaataccc ttcctgtgca atttatgtgg tctacatagc aattaatctc gctgccgatt
    37621 tggatgccat atttctcgta aggttttgcg ggaatgaaat gcctgagatt gaaggggtct
    37681 tcaacaataa gatactgctc agaatcctga agcgttatga gtttcagaac cttgaaaata
    37741 taggaattgc cttcaacaag tttcagttcg ttgtcagggt ataaatagga ggtcatccgt
    37801 caaaagtaat aattagccca aatattttga ggcatccgga ggttttcctg atcagtttat
    37861 cctcaccacc ctggttacat ccttcacttt tgagatgcgg gcgataatca tttccaaatg
    37921 actttttccc gacacattga tggttataat cccttcaaac cggccgtttc tgctctcaat
    37981 attgattgag cgcgtgttgg ccttcaactc atcggaaagt accgtggtaa ttgaactcaa
    38041 tattccgacc cggtcatttc cggtgatttt tattgtagcc agatagttgg aaacttctgc
    38101 attctccgcc caccgggctt taacgaccct gtataaatac cggctatgca tctggtgagc
    38161 atttgtacag tccaaacgat gtattttaat gccttcggta acggtaacaa aaccgaagat
    38221 ggcgtctccc attaccgggt gacagcacct tgccagtttt acatcactca ggggcgtatc
    38281 atcattgatg atcacaatgg cttttgaaac agattcagaa gattttcttg ttttcttctc
    38341 aacggtcttt tccgctggtt ttatttcttc cggtttggct gatgggaata gtgcttcttt
    38401 aatttcctga atatctatgc gttctattgc aatatcatga aatagatcca gcgaattatc
    38461 ggctttaaaa tgtgtgacca gtttatgaat tgcctcatca gcattggtga tttttaactg
    38521 gtttaatttt cggagaagaa tatccctgcc gatatcagcc tgtctgaatt ctgcttcttt
    38581 cagattacgc ctgatcttgt tcagcgcttt tgaggtgatg gcgatgttaa gccagtccct
    38641 gctgggacgc tgatttttcg atgttacgat ctcgacctgg tctccggttt ttaaaatata
    38701 tttcaggggg acaaaggaat tattgacttt ggctccggta caatgatttc caacatcaga
    38761 gtgtacttca aaggcaaaat cgagagctgt ggccccggca ggtaattttt tcaggtcccc
    38821 ctgcggggtg aaaatataaa tattcctgtc gaaaggttct atttttgtgt tcctttcggt
    38881 ttcgcgatct accaaaagat aattttccag aacggtccgg atattcctca atctcagatc
    38941 ggcgccggat tcgctcccgg aagctgattt atatttccaa tgggcagccc ggcccttctc
    39001 agcatcttca tccatccgtt ttgaacgtat ctgaacttcc acccattttc cctgcggccc
    39061 gatgacggtt gcatgaagcg attcataccc gctgctcctg ggagccgaaa tccagtcgcg
    39121 catacgggat ggattcggct tgtaaatgtt tgtcaccagg gaatatactt cccaacagtc
    39181 gcgcttttcg ttcgcaagga ggctgtcggt tatgatgcgg atagcaaaaa aatcatatac
    39241 ctgctcaaac tcgatgtttt gctttttcat cttactccag atcgaaggta ttgatttggt
    39301 acgtgacttt atctcataga tcaggctggt cttcttaagt tccctttcta taggttttaa
    39361 aaactccttg atgtataact tctgcgattc ttttgaatgt tcaatcttat cagcaatgct
    39421 tttatatatc tccggataaa cgtgctgcat tgctaattct tcaagttccg atttaatttt
    39481 atacagcccc agccgatgtg caagcggagt atacaggttg atggtttccg ccgatatctt
    39541 tcgctgaatc tcaaacggca ccttttcaat tgtccgcatg aaattcagcc tgtcagccag
    39601 cctgatcaga attacccgga tatcatccgt cattgttaaa atcaactgga tgaagttctc
    39661 ggcctggatg gaaattttct ccgtatgcaa tcctgatatc cgatggagtt gtgaaaccag
    39721 tccggatgta ttatccccga aatttttttc gatttcagct aagctttttt cgttggttaa
    39781 agtatcctgc aacaatgccg caataatgca ggaaacgggc atttccatct ccatggccag
    39841 gatagaggcg acagcacagg aatgatgcac tctactctga caaacctcag gagtttcatt
    39901 ctgaaatacg gaaatcatat atttataggc acgccggatc tgctttttct gatccgtatc
    39961 tgccatacga tcacaagata gcataagcag ccggtaactg cggtaaacgg tattgttttc
    40021 accctgtgcg cgtacctgag ttgtgttttt catagatgca aaattacaat gagtaatatc
    40081 gaaaaaaacg cccggaaatt taccgggcgc catttcatca aaaggcagga taaaactatt
    40141 tgcctggttt aataactacc tttcctgtag tccttttacc gcccgttccg gctggtgttg
    40201 tggtggctga ttttgaggta tttgaagggg acggagtggt cgttgttgat ttcacagccc
    40261 ccttttggcc tcccgcaaca tagtattttt gagcttcagg aaggaatttc ctgatttcag
    40321 caatacggtg ttcatcactc ggatgggtgc ttaaaaattc gggcgtctta ctgccccccg
    40381 aactggccat ttcctgccag aaggataaag cacgctgggg atcgtaacct gccatagcca
    40441 tgaaaataag tcccagtttg tcagcttcca gctcatgctg acgtgagtaa gccagggtgc
    40501 ctaactgcga tccgaccccg aaagctgcca aaaaaatctg cctcgtttgt tccggctttt
    40561 gttgcatata cagttcaagc ccgatgccgg ctgcctggac agccagttcc tggctcatcc
    40621 tttcatttcc gtgttcagca atggcatgag caatttcgtg cccgagcacc accgccaatc
    40681 ctgcatcatc tttagtaagc ggcagtattc cggtataaac tacaaccttt ccgcccggca
    40741 tgcaccatgc atttacatcg gtgctgttca ctgtattaaa ttcccagtta taaccggcta
    40801 atcgtttcgc cattccgtta tcattcatgt atttaacaac ggctgctttt acccggtcgc
    40861 caacaactga cacttctgca gactgaaccg ttggtggagt cactgcggga ttttctttta
    40921 aaaatgacgt atagttttcg gcccccatgc tgttgacgat gcttgatggc agcagcttaa
    40981 gctgtttacg gcctgtaata ggcacttttt gacatgaggt aaaagccgcc gacattaaaa
    41041 ttactgtcag caataaaact gttatccttc tcatacagat atctttttgt taaaccttaa
    41101 aactgtcagt taatttttgc tgtaaggttc ctgttgttga gttcaaggca aaccggatca
    41161 agttcgtcgc gggaaccaga cttaacgcta catttacctt ttaaatgtgt gatcagggct
    41221 acctgttcgg cctgctccgg cgtatgacca caaacttcaa tgagcgtgct gattacgtat
    41281 tcaaaggtat tgaagtcgtc gttgtacaac acaatttcgt ggccgtagta atgaccggtt
    41341 tcggtgacat ccctgataag cggtttattc tttgtcattc cctgatccat tgttttacaa
    41401 agatagtcaa atcatttctg tcctgttatt ttcagcagca tctcaaaaat ttatcaaaca
    41461 agccgctgaa aatgtatttt tacggttaca ataactatac caacaatttc gggagaatgg
    41521 tcgagaagat atcttacagg gaattattca gcgattggcc tctattcaaa tataaactga
    41581 gtacgaggct ttccgaacct ttacccggga aagaagccca atacaaatta gcgcctgtac
    41641 accgccagtt tgaaaaggaa agtggcttaa caccggatca tgctgttgaa agcagtgtcc
    41701 tcctaacctt atttcctgaa aacaattcgg taaaatcggt ggttattttg cgaaatgagt
    41761 atgatggcgt tcactccgga caggtcagct taccgggcgg aagaaaagaa tcttcggata
    41821 agagcaatta tcacaccgct ttacgcgaga cgaaagaaga aataggaatt cagccggata
    41881 tgctgatata ttgcggaacg cttaccagcc tttacatttc gcgtagcaat ttcaatgttt
    41941 ttccatttgt tgccaccatt gaatttccgc cttcttttgt cattgacaac agggaggtaa
    42001 aagaactcat cgttttcgac ttgctggata taacaccccc cggcgcaaga atggagaaaa
    42061 agttccggtt gagagataat ttcacattta acgcgcccgg atttcttgtc ggaggacatt
    42121 ttatgtgggg cgcaacggca atgattttca gtgaattcct tcaggtaatt gaagatttaa
    42181 gggccccgcc cctatcctga tgctgctggt tatttctgct ttgaagaatc aaaaaccact
    42241 ttgccattga tgattgtgta aagcacgttg gttttaagaa tatccttttc gggaatcttc
    42301 atgatatcct gatccaggat taccaggtcg ccatattttc ctttttcgat actgcctttt
    42361 tcctcttctt caaatgcagc tttcgcagcc catatcgtaa tactctcaag cgcttccttc
    42421 cggcttaatg catcagtcgt ttgaaagcct tcagcagggg ctccgttgat atctttgcgg
    42481 gcaacaccgg catagaatga cttcagaggt tcaatgcttt caatcgggaa atctgtgccg
    42541 tcgggaagcc atccattctg ggcaagaagc cttttataag gatacgcatt aatcaccctg
    42601 ttgcctaacc tcgtttcggc ccaacccatg tcagaagtcg catgggttgt ttggattgat
    42661 ggtataatgc tgaatttccc aaacatgtat aaatcttccg ggtccacaac ctgggaatgt
    42721 tctatacgcc agcgcctgtc attatgacct ttcaggacta tcccgtagtt tctgagaata
    42781 aaagcaacgg cagagtcgcc gatagcatga gtacaaacct gataacctgc atggtatgct
    42841 ttttcacagg tttcacgtaa gacgtctttt gaaattacca gtactccaaa atttgaacga
    42901 tcgtcagaat atggtttttt gaggcaggct ccccgcgagc ctaaagcccc gtcagcataa
    42961 agcttgacag accggactgt caactgaggg gttttataga ttcctttatc aagaaaagtc
    43021 ctgaaattct cttcggtagg attcagcatg gcatttaccc gaatggacag tttccctgcc
    43081 ttttgaagcg aatccaggcg aatgatgtct ttccggtcga ggccggcgtc tgtcacactt
    43141 gtaaggccgg cagcatagca gcggccggcg gccgaaaaaa gcatatcctc agtttcagcc
    43201 tcagaaaggc ccggcacggc cgcttttatt ttatcggctg ttccctcaag aaaaatgcct
    43261 gttagcctgt tgccgtcgta aatagcttca cccggcggaa tagcgccgag attcgaccgc
    43321 ctgattgcct ctgtactggc gagtactgca tggccatcga tccgtgtgag cacaaccggt
    43381 atacccggaa aaagacgatc cagctccttg ttatcaggga acctgcgacc gggccattta
    43441 ttctggtccc atcccctgcc gagtatccac cctgatggat gcgcttgatt ccacaacttc
    43501 aactgcttca gcacgccggt gaacgatccc gctgccgaaa ggtccgcgta ccgctgcatt
    43561 tgtgccaggc cataaagatg ggaatgagcg tcgataaaac ccggatacac aaaatgtttt
    43621 tttgcattga tctgaaccgt tgcgtcgtaa cggctcaata tccaatcatc atgaccgcat
    43681 tcaagaattt taccatttct gattacaaca gcgcctgttg tcgttgaaaa tgcactgtca
    43741 accgtgtata taaatgcatt atagacaata agatcagcct ttcttttcat atcatgacac
    43801 gcgcttatgg aaaagaaaga ggataaagcc atgatgatca ggatgagatt tgagatgaac
    43861 tttgcaggtt tcatgatacc ggacttcttt gactgcaaaa gtattgaaat tatgagggcg
    43921 gcagattgtc agcatcattg tttgcggcta ataatcaacg actgatcatg aatatttgcc
    43981 ggatttaaac aggagtgtgt tagtatccgc tgaattatct tggatcagat cctttgcttt
    44041 ttattacttt tgtaactctt caacctatat cgtacagatg gacaacaaaa cattctcatt
    44101 aaagttctac ttgattgtat caatggtagt tgcggctgca ttcatgcgcc tggtaccgca
    44161 ttggcctaat tttaccccta ttgcggctat tgccttgttc gggggtacct atatcaaacg
    44221 gaaagagctg gctttcctga tccctgtatc agctctttta ttaagcgatt tgttcctcgg
    44281 gttccatagt actatgatac cggtatacgc cagttttctc ctgattgtat cgctgggatt
    44341 tgtactgcgc cgcatggttt cggtgaaagg tattgttacc gcttcactgg catcatccct
    44401 gctgtttttc ctgattacaa attttgctgt ctggtattcg ggaatgatgt tttatccgca
    44461 caccccggca ggtttgatgg cagcttatgt tgcaggcctg cctttcttct ggaacggatt
    44521 agccggagat ttgttttaca acggcatcct gttcggagct tattatttga ttaccaataa
    44581 ggtatctgtt ttcaacagag cctaaacatt cgccctgatt tataagaaag cccccgaaaa
    44641 ttcgagggca ctgaaaaagt ccatcggaca taattgttaa aataaaagca gtaaa
//
LOCUS       DAOPYV010000018        44243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_710_length_44243_cov_12.300557, whole genome shotgun sequence.
ACCESSION   DAOPYV010000018 DAOPYV010000000
VERSION     DAOPYV010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 44243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44243
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_710_length_44243_cov_12.300557"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..2668)
                     /locus_tag="PKI35_03770"
     CDS             complement(203..2668)
                     /locus_tag="PKI35_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX43532.1"
                     /translation="MKKFTLLLTAIVFSLVGLQAQTLPYNDDFESYTVGGFIAAQNST
                     WWTTWSNNPGSSEDGEISTAFANSGTKSVLCDAPTSSTASDLILKLGNKTTGKYELKW
                     MMYVETGKCGYYNIQKTEAPGTEWAFEIYFRTSGAGELYAGSETAIAFTYPKDTWFEV
                     KQLIDLDADNIKLYVNGTLVNEWPFSYTSSATTGMTQLGGIDFFAGALNGTSETPKYF
                     IDDLTFQVMPTVLLLEDMESYAVDSYLAVGNPDWFTTWSNAPGTGEDAIIKDTYSHST
                     TKSALIDETGGATDLILKLGDKTTGAYELTWWDYIPTGKCGYYNIQKTQAPGTEWAFE
                     LYFRTNGTGELYAGSETALTFSYPKDTWFEVKHIIDLDADNIKLYVNGTLVHEWPFSY
                     TNSATTGMKQLGGVDFFAGALNGTSEVPVCYLDDVYLAQLGAPSDPIIEVNPTSMMAW
                     LEPGNTQTKQLTVSNTGASALNYNINIIYPGDKKDQIVNLAAPTHTLQHTSGCSAVSS
                     NGGSPETDATYVLHYDGDNGSAIGWSSVPMTATVAAKFPNMLTVPRAGMTIESVDVYI
                     NDLNASGSNEMTLKIWGMGDAYGPGALLHSQTFTPGGTAWEHLVLTTPVLITGEDIWV
                     GYTFTQNEADVFIPGCDAGPNDPNGDYISTGVGWSHLSNNAALPYNWNIRANLTGDPI
                     AQWLVVSPTFGSVDPQGTQNLNVTFNATNLTDGLYNGIIRILNNDPANNQLDVPCTLS
                     IGVGMNENDKVGVMIYPNPAKDMLNIVTNDAVVKVTILNTNGQVVYSGNQNNINISAL
                     PQGIYSVRTQTVKTNSVIKFVKK"
     gene            complement(2836..3795)
                     /locus_tag="PKI35_03775"
     CDS             complement(2836..3795)
                     /locus_tag="PKI35_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246595.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNX43533.1"
                     /translation="MSAIIYPWGHQKRFNSYSEYFRRTFGHRVQKVAIDAGFTCPNRD
                     GHKGYGGCIYCNNNAFNPSYCQPQKSITQQISEGIGFHRVRYRRAANYLAYFQAYSNT
                     YASLPELKRRYEEALSYPGVIGLVIGTRPDCIDDEKLEYLAGLARKYYIIVEYGVESC
                     FNQTLEKINRRHTFSESVTAIRKTSEYGIHTGAHIIFGLPGETEEDMLSEAEILSLLP
                     LNTIKFHQLQIIRNTLLEKEYLQNPERFNLFPLDEYIDFIIRFIERLNPAFVIERFSG
                     EAPPRFQSGQVWGNLRTDQVQKLIENELEKRDTWQGKLYNCQA"
     gene            complement(3855..5870)
                     /locus_tag="PKI35_03780"
     CDS             complement(3855..5870)
                     /locus_tag="PKI35_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNX43534.1"
                     /translation="MKLLYTKNQLLMFKQHPKGLIVLAIANMGERFGYYTMLAILTLY
                     MQAKFGLTSSTTSIIYGSFLALVYFLPLFGGIIADKVLGYGKTILLGIGVMFTGYLLL
                     AAPSEPNTGGYIMMFSALALIAMGTGLFKGNLQALVGNLYEADIYKNKRDIAFSIFYM
                     FINIGAFFAPSAANSINKAILAQEHFTYDASVPKNYVKLNDKVVDRTVFLEDIFKNNT
                     KALATHKDSVQVIEKAKKKQAVIFQEDKDKALFNLKSAGLYQLTNAGLLKDSNSLSLQ
                     EYRMLESKRPEDAATKNAIIDKINKISITDPKLYGDSSNPELFASNFGKAYVVKSLTK
                     SYNWAFALACISLIISVSVFLGFRKTWKSVDKTHKQQLKEAKLSGNNADVVVLTPKQV
                     KERVIALLLVFFVVIFFWMSFHQNGLCLTFFARDYTTSMISPVQNMMFSLLALLPFIL
                     FTYGIYQATIGLFGGEKKPLTGIILSAAGLAGLLLYYFIVVKDAIGLVPITPQIFQQF
                     NPLFIVLLTPVSVALFAFLGKKGKEPSAPKKIGFGMLIAALGFIVMIIASMGLPAPSD
                     LQGVSNVLVNPGWLIGTYFVLTIAELFLSPMGLSFVAKVAPPQYKGMMQGGWLAATAI
                     GNYLVGVLGMFWEKLPLYAFWSVLVICCLLSASFIFSIMKRLEKATE"
     gene            complement(5970..8117)
                     /locus_tag="PKI35_03785"
     CDS             complement(5970..8117)
                     /locus_tag="PKI35_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNX43535.1"
                     /translation="MRYSAFHIRFAGNMLRIKAAGITGFIMLTGIPGLVSAQQPDSLQ
                     PPDNDLHQDRIEYISEQTDSETDFSEWTDELRSLSFRPINLNSTEEEELRRLPFLNDQ
                     QICNLLDYHRKYGAFASIYELQAIDGFNETFILQMLPYITLSDPISEKFNIKRAFKYG
                     RPELMLRYQRVFEKQQGFTKVADSIRAQNPEKYFLGSPDAFYVRFTYTYKDRMHLGLV
                     ADKDAGETLFPKSDTLHKGFDYYSFHFYLRDIGKLKHLAIGDYHLQFGQGLTLWTGLS
                     FSKSPGAIAMRKRAPLVSPHASANEYAYFRGAAATFSINRVDITGFYSNRNIDANYFD
                     ADTAAGTEAYLTSIIETGYHRTPGELSDKASVNQQVAGGHLNYKGQRLRIGATGYYSL
                     LNHLYNPRQSLYNRFEKTDRESIFLGIDYCYSYKRLTIFGETAQQLHAGTATVNGLSL
                     SPDPRFSMSLVYRNYKKDYTNPYAAAFGESADNTNEKGLYFGLNATPFRNVAIVAYSD
                     LFRYDWLRFRVDAPSHGDEYSVRIQYSLGRKGDITLRYRQVHTPLNFSREEGRINSVE
                     DVTRNYCQVLLNFQVLPWLTLRNRLYYLHRETPETNPERGFFISQDLIFRPEEKPFSA
                     VLRYALFDADSYDSRIYAYESDMPYSFSVPSFSGKGSRFYTMLKISILRKADIWLRYS
                     VTTYFDRQQISSGISQIDGNTKSDVRVMMKVRF"
     gene            complement(8133..8948)
                     /locus_tag="PKI35_03790"
     CDS             complement(8133..8948)
                     /locus_tag="PKI35_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43536.1"
                     /translation="MKYFLVSVSVLMLFVTARAGDHWRPAGARSAAMGMVSVSFPDSW
                     SIHNNQAGMAFYNRPVAAFYYENSFLLKELGYQAAAFTLPTRYGVFGGNVAYTGDAVY
                     NEANVGIAYSRAFGNHFAAGIQLDYLHTGLSNEYGSSGLATFEAGILAKLTSNLTFGA
                     HVFNPLSRKVSAHCDERIPAIMRAGFTYTFSEALLLSAEVSKNSLQPIQFMAGTEYRF
                     FKKGFARIGLATNPFKYTFGFGLEIKNFTFDISSSIHEVLGYSPQVSLQYAFR"
     gene            9056..9721
                     /locus_tag="PKI35_03795"
     CDS             9056..9721
                     /locus_tag="PKI35_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNX43537.1"
                     /translation="MITANTLDFLSRLKENNNREWFQQNKKLYEQVRGGYEKFIAELI
                     PDIAVLDPSIGFPDLKDCLFRIYRDVRFSPDKSPYKSHFGAFIGKGGRKTTGAGYYVH
                     VEPGNSLVAGGIYMPQPDILKLIRNEIYFNSSDFKKIITEAAFVNTFGRLDDFDKLKL
                     PPKDFPSGFPDIDLLKYRSYVVGRPLSDEEVLSPGYKKNILEIFKAMLPFHEFLNRAI
                     DNR"
     gene            complement(9800..11782)
                     /gene="gyrB"
                     /locus_tag="PKI35_03800"
     CDS             complement(9800..11782)
                     /gene="gyrB"
                     /locus_tag="PKI35_03800"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNX43538.1"
                     /translation="MNEITKGPNGSSNGNGNGNYTAENIQVLEGLEAVRKRPAMYIGD
                     VGVRGLHHLVYEVVDNSIDEALAGFCDTIEVVIHADNSITVTDNGRGIPTDFHEKEQK
                     SALEVVMTVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSDQLVVTVYRDGKIFEQDYS
                     KGKPLCDVKIVGTTQVTGTRVHFKPDNSIFITDHYDFTILSSRLRELAYLNRGIKLTL
                     IDEREKDESGKDVSESYFSNNGLVDFINYLDETREKLIPEPIYMEGEKNDIPVELAMQ
                     YNTSFSENIHSYVNNINTHEGGTHLAGFRRALTRTLKTYAEKSGMLEKIKFDISGDDF
                     REGLTAIISVKVMEPQFEGQTKTKLGNNEVMGAVDQLVAEMLNYYLEEHPREAKMIVN
                     KVILAATARHAARKARELVQRKSVLSGSGLPGKLADCSEKDASLCELFLVEGDSAGGT
                     AKQGRDRKFQAILPLRGKILNVEKAMQHKIFDSEEIRNIYTALGVTIGTEDDTKALNL
                     DKLRYHKIVIMTDADVDGSHIATLILTFFFRYMKELIENGYVYIATPPLYLVKKGNTE
                     KYCWTEEQRIALVNEISKDGTEKGIHIQRYKGLGEMNSEQLWTTTMNPDFRTLRQVTI
                     ENGSEADRIFSMLMGDEVPPRREFIERNARYANIDA"
     gene            complement(11984..14668)
                     /locus_tag="PKI35_03805"
     CDS             complement(11984..14668)
                     /locus_tag="PKI35_03805"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNX43539.1"
                     /translation="MQRITAFLYLPLIFLAICNGLSAQPNPSVKIYRTSRIQLKAPVI
                     DGVINDDEWNGAEWQGNFVQREPDDGASPSQQTAFKLLYDDNNLYIAVRAYDSVPGKI
                     VRRLSRRDNSDGDWVGIIIDSYADKLTGFGFGVTAAGVKFDLMVINDGNSDETWDAIF
                     DAKTSVDSQGWMAEFRIPLSQIRFSSKEEHNWGLDVFRYIYRKQEMSLWQPVARNAPG
                     FVSLFGELQGISGLKPHRDIEILPYLMASEELSEKEEGNPFATGKKFTPKVGVDGKIG
                     ITNDLTLNFTVYPDFGQVEADPSEVNLTAFETYFSEKRPFFIEGKNIFDFSLTGGDGD
                     DTRNMLFYSRRIGRQPQYNNDLYDNLLDDEYADVPASTNILGSFKLSGKTNKGLSIGI
                     LESITQSERASIDRNGDRRKVTVEPFTNYTVARLQRDFSKGETILGGMFTATNRDIND
                     SNLEFLPRSAYTGGVDYTRYWKNKTYYLMLKGVFSSLSGTRECITQLQQSSAHYFQQP
                     DQDYMKMDTSITTLNGYGGTIMGGKAGQGHWQYMGWLTFRSPGLDFNDVGYMRRADEI
                     QQVAWVGYRIYKPFSIFRYLGINFNQWNGWNFGGEWTYAGGNINGNAQFKNYWQFNFS
                     VNADSRSLSQSILRGGPALFMPGDINNFIYLSTDNRKKLFFEFDASMNRGFCDYYRTN
                     GIDFGIVYKPVNSVSVTLMPGYSYGHDDLQYIDLLYNYSVPSSDGQERYVLARLDKKI
                     FSLSARVNISVTPDFSIQFWGQPFLFSGKYSRYKYVTDPRANDYYARFREYAANEISR
                     YYSETDEDFYYSIDENSDGTEDYNFQEPDFRFMQFRSNLVARWEYKPGSVLYLVWSQG
                     RTGYEEDHRFNIHENMKELFDTHPQDIFLIKLSYIIIF"
     gene            complement(14852..16594)
                     /locus_tag="PKI35_03810"
     CDS             complement(14852..16594)
                     /locus_tag="PKI35_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43540.1"
                     /translation="MNISPRLDMPNRQYSKVTKLRLAVAIMAMIPVLVSAQQPKYTEE
                     ITVIATYKPEIPDAFKINQNPVNNDTSTTIPVMTYNIQPRPATIQLAIEPLPSPKLVA
                     EPINKLYRNYLRGGFGNYATCYGELNASSLRSKKGMVGVRIKHLSSAGTIKDFGPPAN
                     SHQLADLTGERYFSDHVLGAEAYFNRDAIHFYGVPDSTLEKFDIEKDSIKQRYISAGG
                     NLTFRSAYKGNNKLNHAVSLGYSYLEDNFDASENNIHFNAAADKAADLFNLKEKQTLG
                     LTADVQFYNQRNRFFETTNSVVVMLQPFIGVKYNEYSFKAGFSFYVQGDTVTKGHLYP
                     YLEARLEAIPNALQIYAGIDGSMHRHTLKETACENPFIKDSLEFRYVNEKFRAYGGFS
                     SNISKSFNLNGSISASSVENNPFFVGETSSLNNTFMLKYDDVSVMKLRAELEYVKSEK
                     LNLALSGAFNRYYKLLYEKHPWYKPEFVIGFNSRYNIQDKIILKLQADYNGPVWALRP
                     ATSEPDETPGTSGKISEKLDGWFDGSLGIEYRFNKALSFWLNFNNFTNTRHWLWYNYP
                     SYRFNVLGGVSFSF"
     gene            complement(16617..19640)
                     /locus_tag="PKI35_03815"
     CDS             complement(16617..19640)
                     /locus_tag="PKI35_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX43541.1"
                     /translation="MNKHFPVIFFLLTLLTVFGAQAQQTQVYRWPQAEYNNAVDLYSR
                     AKYGSALAIFDRIASMGNDNLSAGAQYYAALCAGQLFHPDATERLETFIQKYPEHARV
                     NNAFFELGKQFFNSRDYRNAMLKFALVDIYELGQEQADEYHFKMGYSAFKTDNLATAK
                     EELAKVKDKPNRYHAPANFYYASIAYLEKNYETALKHFEKIENDETFKDVVDYYIVQI
                     YSMQGKYNELLARSVPLLEQQGNTRMNIEMARLTADAYYHIENYQEAASCFDRYLNAN
                     PANISRNDYYEIAFTAYYNGDFNKAVKYFQLVATNQDSLSQNAYYHLGNSYLKTGQKR
                     YAHNSFLSACKIKADPALAEDALFNYAKLSIELSYNPYNEAVKALQQYINEYPASSRL
                     DEAYGYLADLYTITRNYKDALTSLQQIKKRNARLDAAYQKISYYRAIEQFSSTDYNGA
                     IGLFKQAQETNSDKQVQAASAYWMAESYYRLGEWDKAAEVFNKFLVSPGASAQPFFNT
                     ANYNVGYCYFKQQEYGNAALHFRKYLTGKPVETKLVADANLRLGDCYFMQKDYGTAIT
                     YYQKAVTLKVPDADFAVYQIATAYGVQGEMQQKIDWLQKLLKDYPRSTYFDDAYYETG
                     NTYTIINRNEEALSYFEKVVNDYPKSSYVKKSLLKTGLIYFNQNRDNDALATLKRVVK
                     EYPGTPESREALASIRSIYVDQNKVEEYVDFTKTVPQADVSRTEQDSLTFIAAENQYM
                     NGNCEKATESLDKYLARFPEGSFTIKANFYKAECDYRAEKYDQALKGYLFVLNNQRTA
                     FSTTAALKAARIQYAQKDYSEALENFIRLEEISEQPIHVTDAITGQMQCNYLLGKYGL
                     AIQAGQKLLLKENLQQNLAVEAHLYIGRSAYALGNSELARKEFEETLKLSQNEMGAEA
                     KFMTAQLQYDAAAYDQAEKTLFALAEDFASYDYWVAKGFILLADVYVKKNNTFQAKQT
                     LQSIIDNYEGQDLVVTAREKLNAINETEISSPVNQ"
     gene            complement(19835..20743)
                     /locus_tag="PKI35_03820"
     CDS             complement(19835..20743)
                     /locus_tag="PKI35_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX43542.1"
                     /translation="MKSYRKLFSTLIIFIFCISLAAAQDIPNAGFEEWNNSEPVDWHT
                     TNQPFFNTVLKESDNPQEGAYSALLQVVTVTVPFVGTYSLPGVLSTAEMSVDVYNQTY
                     NLSGGYPFTGMPNKLSGYVKYQPVNNDMCAFGWALSKWQNGTRDTIGFAYQYIAEELS
                     DWTYFEIPIEYGIWEAPDTMNILFLCSNILDGQTHTGTKMWVDNLSFLYGPVGIEGVT
                     SGNGYRVYANGQTRQLILETSFSESRPLTIRLFSINGALLAEEKRSMSHSTEFIDISS
                     LKTGIYVLRIIDGNGVTDTRKITIVN"
     gene            complement(20812..21225)
                     /gene="rnpB"
                     /locus_tag="PKI35_03825"
     ncRNA           complement(20812..21225)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKI35_03825"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(21410..24061)
                     /locus_tag="PKI35_03830"
     CDS             complement(21410..24061)
                     /locus_tag="PKI35_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNX43543.1"
                     /translation="MIKRFFILISLALIVVPAWATHQRAGEITYRRIGDLTYEVTIIT
                     YTQAYSLADRNELPLKWGDGMSSSLPRTNGPAGTTPAGYPSDHIGEIIGPSSDSIRKN
                     IYTGQHTYAGPATYLITLEDPNRNAGIMNIPNSVDVPFFLETELVVNPFVGINNSPLL
                     TLPPVDKACVGHPYLHNAGAYDPDGDSLSYKFVVCRGEGGEPIPGFKMPNEVDQANPS
                     TFTIDPATGNILWDKPTMLGEYNFAFQIEEWRNGIRIGYVTRDMQIRVLACVDEAPVI
                     KPLMDTCVIAGDSLTYAVSATDTDLDKIALTATGQPFILAESPAVFEQSSDSLGLATG
                     LLQWRTACSHIKRTPYQIYIKAIDNARPVSLFDIYTLNISVIGPKTLNINAVPVGNSI
                     NVSWSPNGCPDAVSYNIYRRNGFYGFVPGYCETGVPSSTGYRKVASVNGWGNTLYIDD
                     DKGAGLVHGVDYCYIVTAVYPDGSESIASDEACAKLKKDVAIITNVSIDKTSVTDGQV
                     YLAWSKPTEIDPVQAPGPYKYLIYRSDGLTGSNLALIDSLSSLDDTIYIDHPVNTSDD
                     AVSYRVDTYNDTPGLRFLIGASQKASSVFIQTAPGDEKLRITFNYQTSWLNSQFIVYR
                     LNNDTHLFDSIGTTDLSYYIDRDLINGETYCYKVKSIGSYGTEGITDPLLNWSQETCG
                     IPIDNEPPCPPVPVLKTDCNTFINTLSWSNPDPACSSDIAGYYIWYRPPETEQLSLID
                     SVDGAGNTTFLHGPLSTIAGCYAVAAFDSAGNVSAMSDTLCIDSDTCRGYRLPNVFTP
                     NGDTYNDYLVPFPYSSVEKIHLEIYNRWGRLVFKTEDPAIKWDGKILNTGQPASDGVY
                     YYVCDVYEIRLAGIVKRTIKGTVTIIR"
     gene            24302..26521
                     /locus_tag="PKI35_03835"
     CDS             24302..26521
                     /locus_tag="PKI35_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNX43544.1"
                     /translation="MYEADVDKENKEIIKRYRSLLRAWNSNKGVRDKNRVKEAFQVAS
                     EAHRTMRRLSGEPYIYHPIEVARIVAGEIGLGETAIICSLLHDVVEDNEDYSIKFIED
                     KFGVRVAYIVDGLTKIKDGYETMESSLQAENFKKMLLTLSDDVRVILIKLADRLHNMR
                     TLDAMPARKQKNIAAETLYLYAPLAHRLGLYAIKSELEDLALKYTEPEVYNTLARKLH
                     DTEYERSRFIQRFILPIKKSLAANGISFEIDSREKSIYSIWKKMQTKEIPFEEVYDLF
                     AVRIIIDSPPEAEKINCWQVYSIITDHYSPKQNRLRDWISTPKANGYESLHTTVMSQT
                     GQWVEVQIRTRRMHEIAERGYAAHWKYKQLNERERSQETGLDQWLNKVREILRTPDSN
                     ALNFLDDIKLTLFSDEVFVFTPRGEIKSLPAGSTVLDFAYNIHTQLGNSCIAAKVNHK
                     LVPLNYTLKSGDQVEIIASKKGMPKEEWLNYAYTVRAKTYIKETLKEQRKQFVEEGRN
                     QLESYFKELKLRFTRANIARFMAYYGLASHIDLYYKVAKNDISLNDVQDCFLDHKADL
                     REAAPETGETQVVPEIPLSRMLPAIPDLDDTDFTVSKCCYPLPGDDIVCYVSPDGFIQ
                     VHRTHCPEAIELMSRHAKRMISPRWPNRHLNTYQAGIKATGIDRKGLIFELIHVISEE
                     MHFNVTLFHIETSGDIFEAVISLFVHDTIDINRLIGEIKKISGITNVSRIMKFEKFS"
     assembly_gap    26621..26720
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(26758..27318)
                     /locus_tag="PKI35_03840"
     CDS             complement(26758..27318)
                     /locus_tag="PKI35_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNX43545.1"
                     /translation="MSRNYKFHNPEGVYFVSFAVVEWIDVFIRNEYKEILLESLAFCQ
                     KNKGLEVFAWCIMTNHVHLVFRSAGDNKPEDLLGDFKRFTSRAIVQAIMGNPKESRRY
                     WLLEQFARAAAQSSNVKHHQFWRHDNQPVELWSNKVLIEKIRYIHNNPVEEGLVFKPE
                     DYLYSSARDYSGNKGLLDNVIVVDLF"
     assembly_gap    27363..27462
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            27624..27788
                     /locus_tag="PKI35_03845"
     CDS             27624..27788
                     /locus_tag="PKI35_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43546.1"
                     /translation="MLRKVLGVAGFYYHAKAFQPPAERKVRAAAGIGEIKKISGITNV
                     SRIMKFEKFS"
     gene            27986..28465
                     /locus_tag="PKI35_03850"
     CDS             27986..28465
                     /locus_tag="PKI35_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNX43547.1"
                     /translation="MSDMNERVNFDTVKKIFTDYLEKLGHRKTPERFTILKEIYNTDS
                     HFDIETLYIQMKNNKYRVSRATLYNTIDLLLNCNLVTKHQFRNNCAQFEKSFKFQQHD
                     HFINLDTGEVLEFCDPRIHEILLSVEKSLGVKIEHHALTFYGHKAGSDDKVATVKKD"
     gene            28553..29842
                     /locus_tag="PKI35_03855"
     CDS             28553..29842
                     /locus_tag="PKI35_03855"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNX43548.1"
                     /translation="MKIDVLLGLQWGDEGKGKIVDVLTPRYDIIARFQGGPNAGHTLE
                     IGGDRHVLHTIPSGIFHADKINVIGNGVIVDPLILHNEILALRAKGVDPRLNLRISKR
                     AHLILPTHRLLDAAYESAMGKGKIGSTLKGIGPCYTDKTARFGIRCGDILAGDFLKKY
                     KTLKDRHLRILKSYNFDLAKQEIDKMPVDEYEKHWFDAVESLKEFELIDSELFINHAL
                     SEGKRILAEGAQGSLLDVDFGSYPFVTSSNTITAGVCSGLGVPPTAIGKVYGVFKAYC
                     TRVGSGPFPTELSDETGELLRTNGNEFGSTTGRPRRCGWLDLPALNYAIMLNGVTDLV
                     MMKADVMNDFDKVKICTAYKIEGMENPELTFETLSGNVKPVYRIFEGWKTSLEGLTNF
                     GALPAKLSEYIRFIEKSVNLNIDILSAGPGRHQTIIR"
     gene            30014..32497
                     /locus_tag="PKI35_03860"
     CDS             30014..32497
                     /locus_tag="PKI35_03860"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNX43549.1"
                     /translation="MAKSLGKLVKFLFLTAVLVMNAGHAQSGIVAGDTTIRTRKVIEA
                     LRIESAPKIDGYLDELFWQNLPVARDFTEYAPVNGIKPPFRTEVRFGFDDIAFYASAV
                     MYDPHPDSICREMGKRDQIELLNTDYISFDILPYNDALNMYEFKISPIGLQNDTKYSA
                     LGQDVNWDAVWESAARITDSAWIVEVKIPFSALRFSTAEKQVWGINMWRNLKRRNEWS
                     TWCYVDNTVQDDLFKYYGEVAGMQNVDPPLRLSVTPYIAGYVEKNPSRQQWSSLARGG
                     MDLRLGINESYTVDMMVIPDFGQVQSDDRVLNLSPFEIRYDEKRQFFTESTEMFEKCG
                     IFYSRRVGSTPRNFNSAYDQAEQDETVKMNPDQARIVNATKISGRNANGLGAGLFNAM
                     TANTWATLENTETGSTRKVLTQPFTNYNVLVLDQNLRNKSYVSLINTNYYSPKDSYAA
                     NVTGVESRLSNKKSTFVFFGKLNVSNKIQKGLKTATGHAMEIDISKPNGKFQYYLVRA
                     QADKVYDPNDMGFLLNNNYASNILHLYHMVMRPRGIMINTITRFSTTYNTLFEASKLK
                     CLTFEAANKTTYSNYWVTHFVLSASPLGFHDYYEPRLWGFMYHKPAYYSFNAIITTDS
                     RKPFYVKSVTEFTITPGNHSQGWEAELIPRIRFNDKVTLTLDVDFNMLKNDFGWVNTL
                     YDTIGKPMIFFGRRTVETLNSVLSGQYTFSTKTAVTLRLRHYWSQVDYDSFFLLNGNG
                     DLESSDYQGEHDINFNAFTADAQFVWYFAPGSELSFVWKNAINTFDETMIRNYFDNLE
                     KTFSTPQTNSFSVRILYYLDYQYIRKLIK"
     gene            complement(32542..33546)
                     /gene="gap"
                     /locus_tag="PKI35_03865"
     CDS             complement(32542..33546)
                     /gene="gap"
                     /locus_tag="PKI35_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNX43550.1"
                     /translation="MTKIKVGINGFGRIGRLVFRASTLRDDIDVVAINDLIDVNYMAY
                     MLKYDSVHGQFKGTVDVKDGHLVVNGRTIRVTACKNPADINWSEVGADYVAECTGLFL
                     TAEACQSHLNGGAKRVIMSAPSKDDTPMFVYGVNHKKYAGEQIISNASCTTNCLAPLV
                     KVINDKFGIVEGLMTTVHATTATQKTVDGPSAKDWRGGRAAGGNIIPSSTGAAKAATK
                     VIPELKGKLTGMSFRVPTLDVSVVDLTCRLEKAATYDEIKAAVKAAADGELKGILGYT
                     EDAVVSSDFVGDSRTSIFDASAGIMLNPNFVKLVTWYDNEWGYSNKILDMIKHMSTVA
                     "
     gene            34032..35819
                     /gene="lepA"
                     /locus_tag="PKI35_03870"
     CDS             34032..35819
                     /gene="lepA"
                     /locus_tag="PKI35_03870"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNX43551.1"
                     /translation="MENIRNFCIIAHIDHGKSTLADRLLDMTGSISDREKQDQVLDDM
                     DLERERGITIKSHAIRMDYAFEGKNYKLNLIDTPGHVDFSYEVSRAIAACEGALLVVD
                     ATQGIQAQTISNLYLALDHDLEIIPVINKIDSDSAMPDEVEDQIVDLIGCKPSEIIRA
                     SARTGYGVDAILEAIINRIPPPKGDPAAPLQALIFDSLFNVYRGVIAYFKVVNGTIHT
                     DDYVKFVATQREYHADEIGILRLRPEPCPLLSAGDVGYIISGIKTAKEIKVGDTITHA
                     ETPCNNAIEGFKNVKPMVFAGIYPTDSDDYEDLRASLEKLQLNDAALSFEPESSAALG
                     FGFRCGFLGLLHMEIVQERLDREFDMDIINTVPNVMYKVLTTKGDEIEVHNPSGMPDI
                     TTIDHVEEPYILAQIISKSEYTGAIMKLCLDKRGMLKNQVYLTQDRVELNFELPLGEI
                     VFDFYDKLKSISKGYASFDYHISGYKEASLVKLDILLNGDAVDALSTLIHRDNAYDFG
                     RRICEKLRELIPRQQFDVAIQAAIGAKIIARETVKAVRKDVTAKCYGGDITRKRKLLE
                     KQKKGKKRMRQIGTVEVPQSAFLAVLKLD"
     gene            36001..36486
                     /locus_tag="PKI35_03875"
     CDS             36001..36486
                     /locus_tag="PKI35_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNX43552.1"
                     /translation="MTVAEYNSCADLYSDGVYRFILKNIKDDDTANDIVQESYVRLWE
                     RVKDISFEKAKAYLFTTAYHTMIDHIRRNKRYSEMDGTYDTELQSNNAFSDLKEVIDE
                     AVAKLPEIQRTVILLRDYEGYAYDEIGEITGLNESQVKVYIFRARTFLKQYIGKLDLV
                     I"
     gene            36560..37921
                     /locus_tag="PKI35_03880"
     CDS             36560..37921
                     /locus_tag="PKI35_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43553.1"
                     /translation="MKINRDNYEAYFLDYHEGNLNPDEMAAVLVFIGQNPDLKEEFDE
                     FENVSLSVDDSVSFSEKDFLKKNVEIYSGPVTTENIEEYLVAETEGLLPDETLSQLND
                     FVNRNPGFRKDRRIYAHTRLAPAIEIVYGNKHLLKHVIPHENPVNEANCEEFMIRKVE
                     GLLGDDEAGDLDDFLEQNPRFRKEMKFFGLTKLQPDTSVTYREKASLKHTIVPVRRIV
                     YYAMSAAASVALLMGVYFAWERNRPSDKLAENKSAKTAVTPAVIPDLPSDAQHQDNVP
                     DAEIPAVTTSASSGTTVYIAASQPVKVQNNDDRTAVPMLEPLAECRISSKNCIEPEFL
                     FIRQSRFYGSRYIDLYNSVKLSEQIQYASLNAPDKNPVRTIWKGVFNHIEDKVIDDRR
                     TKTEPAPELSIWTLAEASIKAYNNLTHDDLELLLQKDEKGEVVSYALIGDKVNIERDI
                     AKK"
     gene            complement(37985..41212)
                     /gene="infB"
                     /locus_tag="PKI35_03885"
     CDS             complement(37985..41212)
                     /gene="infB"
                     /locus_tag="PKI35_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNX43554.1"
                     /translation="MTIRLSKAATEFNVGVQTIVEFLHKRGHKIDSSPNTKLTQEMYN
                     LLVKEYQPDKATKESSKKIELEYLHHQALSIDDRKAPLPEEPEDRESSELIIKTFDVQ
                     KQAQAKQVTEPVRKPEPQKAVEPVITEPAPAPTPKPTPVPATPSVPVPAPAPTPTPVP
                     TPAPAPAPAVPAPAPAPAEVPTPEPTPEPTPVVAAVPPAQPPVPAPAPAVPLAPVAEK
                     EPEVLQPEQPAVPVQPAPKPEALEPVAETEPETGEPSGPQLKVLGKIDLGQFDQKSKK
                     KKGEKGGKKKAREEAVEVPPVEEKVVEEAIQPPVEETVIPVKEPVAEVAADALKADQK
                     SEIAARQGIYSREDPNFVKTNIPTLEGPKVLGKIELPKPAQRTDGRKPVASSSEDHGK
                     DNKKKKRKRIKGKGSPVETGTAQPKPHEADKQRPKKERRGRIPEPKVELSEEEIQKQI
                     RETLQRLSGTGKSKASKYRREKRLAVSHHLEEEAERIAEEMKILKVTEFVTPNDLANM
                     MNVPVTQIIATCMSVGLFVSINQRLDAETIALLADEFGYKVEFVSVDVQESIGSHEEE
                     DRPEDLSPRSPIVTVMGHVDHGKTSLLDYIRDTNVIAGEAGGITQHIGAYEVKLEDGR
                     RITFLDTPGHEAFTAMRARGAQVTDIVIIVIAADDTVMPQTIEAINHAQAAGVPMVFA
                     INKMDKPGANAEKIREELSKLNILVEDWGGKYQSQEISAKKGTNVMLLLEKVLLEAEM
                     LDLKANPKRKAIGTVIESALDKGRGYIAKMLVQNGTLHIGDVVLAGSVYGKVKAMYNE
                     RNQAIKEAGPACPVLLLGMTGAPQAGDNFNVMTDEREARQLATKRHQLQREQGIRTQK
                     HITLDEIGRRIAIGDFKELNIIVKGDVDGSVEALSDSLLKLSTEQVQVNVIHKSVGAI
                     TESDVLLASASNAVIIGFQVRPSLNARKLAEREEIDIRLYSIIYTAINEIKAAIEGML
                     APEIEEKITCNVEVREVFRITKVGSVAGCMVLDGKINRNTRVRVIRDGIVIYTGALGS
                     LKRFKDDAKEVASGYECGLNIENFNDIKVGDIIEGYEEIEIKRKL"
     gene            complement(41392..42627)
                     /gene="nusA"
                     /locus_tag="PKI35_03890"
     CDS             complement(41392..42627)
                     /gene="nusA"
                     /locus_tag="PKI35_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNX43555.1"
                     /translation="MEHINLVETFSEFKEFKNIDRETLMRILEDVFRHMLIKKYGTDE
                     NIDIIVNVDKGDLEIWRNRGIVADGEIEDENTQIAYSDAIKIEPDFEVGEQVSEPIRM
                     DDFGRREILAIRQNLVAKVQEYEKDNIYRKYKDKIGEIVAGEVYQVWKNEILIVDDEY
                     LELTLPKTEQIPGDFFRKGDSIRAVVSRVEMKKNSPQIILSRTSPVFLERLLESEVPE
                     VYDGLITIKNIVRVPGERAKIAVESYDDRIDPVGACVGMKGSRIHGIVRELKNENIDV
                     INYTTNPQLYIQRSLSPAKITSIQIDEENKRAEVYMKPDQVSLAIGKGGHNIKLAGKL
                     TGYEIDVYRDTEFDYEDVDLQEFNDEIDQWIIDELKSIGCDTAKSVLELDVEELVRRS
                     QLEEETIKEVIKILKAEFE"
     gene            complement(42656..43102)
                     /gene="rimP"
                     /locus_tag="PKI35_03895"
     CDS             complement(42656..43102)
                     /gene="rimP"
                     /locus_tag="PKI35_03895"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HNX43556.1"
                     /translation="MIRESQIRKLAEAHLAGTDRFIVDVIVKPGNRILVFIDSDTSVL
                     IDHCASLSRYIESHLDRDAEDFELNVSSSGIDHPYTLVRQYIKNTGREVIVTLPDEQK
                     ITGTLVAADQKGFDILEKKKEKKIITEIAHHFAYSEIKETKEVIKF"
     gene            43409..43657
                     /locus_tag="PKI35_03900"
     CDS             43409..43657
                     /locus_tag="PKI35_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43557.1"
                     /translation="MGCVSCFSTLLSDQDFPTMSGFRLRLHFSSWSDGMRLLFFNLVV
                     RPGFPDNVGIPASPSLLILVGWDASPVFQPCCPTRIRT"
     gene            complement(43633..43703)
                     /locus_tag="PKI35_03905"
     tRNA            complement(43633..43703)
                     /locus_tag="PKI35_03905"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43669..43671),aa:Gln,seq:ttg)
     gene            complement(43795..>44243)
                     /locus_tag="PKI35_03910"
     CDS             complement(43795..>44243)
                     /locus_tag="PKI35_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43558.1"
                     /translation="LITMPSEAQNLREMLQQCPVTIENGKINYYMPQQAGPYFQLPPA
                     RKPQYVNIITVPWVHPLTKSVKETQSEVLQSLQEFISQPHPNNADKELFLRKLAWCVM
                     LNASIAKQVDTEITEFINQLASDEDAGIKTKAGLVAQLLEDYRGIR"
BASE COUNT        11192 a        10178 c        10736 g        11937 t
ORIGIN      
        1 cgtctcctga cgcgtgcgct gtaaacctgc ggcattactg catctttttc ggcacacgta
       61 tcctggttac gcttgcccgg gcaggagttg gcgcttttcg ttttttctac aaaacctgcc
      121 gactaccgta gatctacatc ggcaggatca taaaaaaaac gccccggtta cggggcgttt
      181 tttgtgatca atggattcgt aattatttct ttacgaactt gataacggaa ttggtcttta
      241 cagtctgagt ccttacggag taaattccct ggggcagagc gctgatattg atattgttct
      301 gattgccgct gtacacaacc tgaccgttgg tgttcaggat ggttactttt acaactgcat
      361 cattggtaac gatattcagc atatcctttg cagggttggg atagatcata acgccaactt
      421 tatcgttttc attcattccg acaccaatcg aaagggtgca gggaacatca agctggttgt
      481 tggcaggatc gttgttcagg atacggataa tgccattgta caggccatcc gtcaggttgg
      541 tggcattgaa cgtaacatta aggttctgtg tgccttgcgg gtcaacgctg ccgaaagtcg
      601 gacttacaac gagccattgg gcaatcggat cgccggtcag attggcgcgg atgttccagt
      661 tgtaaggtaa agcggcattg tttgaaaggt gactccaacc cacaccggtg ctgatataat
      721 cgccattcgg atcattgggg cctgcatcgc atcccggaat aaagacgtcg gcttcgtttt
      781 gtgtaaaggt gtaaccaacc caaatatctt cgcctgtgat aagaacaggg gttgttaaca
      841 ccaggtgttc ccatgcagtg ccgccggggg taaaggtctg cgagtggagt aatgctccgg
      901 ggccgtaagc atcgcccatt ccccagattt tcagtgtcat ttcattgctt cctgatgcgt
      961 tcagatcgtt gatgtataca tcaactgatt caatagtcat gccggcacgg ggtacggtaa
     1021 gcatattggg gaacttggca gctacagttg ctgtcatagg aactgacgac cagccgatgg
     1081 ctgaaccgtt gtcgccatca taatgcaata cgtaagtggc atctgtttcg ggggaacctc
     1141 cgttagagga aacagccgag cagcctgatg tatgctgaag tgtatgggtg ggagctgcaa
     1201 gatttacaat ctgatccttt ttgtctccgg gatagataat attgatgttg taattaagag
     1261 ccgatgcgcc ggtatttgaa acagtaagtt gttttgtttg cgtgttaccg ggttcgagcc
     1321 atgccatcat actcgttgga ttaacttcga tgatggggtc gctgggagcg ccaagctggg
     1381 ctaaataaac atcatcgagg taacataccg gaacctcgct tgttccgttt aaggcaccgg
     1441 caaagaagtc aacgccgcca agctgtttca tgcctgttgt tgcgctgttt gtatagctga
     1501 acggccattc atgtacaagc gtcccgttga cgtataattt gatattgtcg gcatcaaggt
     1561 caataatatg ttttacctcg aaccaggtat ccttgggata ggagaaggtt aacgccgttt
     1621 cactgccggc atataattcg ccggtgccgt tggtgcggaa ataaagttca aaggcccatt
     1681 cagttccggg agcctgggtt ttctgaatgt tatagtagcc gcattttcct gtcggaatgt
     1741 aatcccacca ggtaagttca taagcgccgg tggttttgtc tccgagtttc aggataaggt
     1801 cggttgcccc gcctgtttca tcaatcaaag ccgacttggt tgtgctgtgt gaataggtgt
     1861 ctttaataat ggcatcttca ccggtgccgg gggcattcga ccaggtagtg aaccagtcgg
     1921 ggttgccgac ggccaggtag ctgtcaacgg catagctttc catatcttcg agcagaagaa
     1981 cggtaggcat aacctggaaa gtgaggtcat caataaaata tttcggagtt tcactggtcc
     2041 cgttgagggc gccggcaaaa aagtcaatcc cgcccaattg tgtcataccg gtagtagcgc
     2101 ttgaagtgta gctgaatggc cattcattga ccagtgtgcc attgacataa agcttgatgt
     2161 tatcggcgtc aagatcaata agctgtttta cttcaaacca ggtgtccttg ggataagtga
     2221 aggcaatagc agtttcgctg cctgcatata attcgccggc gccgctggtg cggaaataga
     2281 tttcgaatgc ccattccgtt ccgggggctt ctgtcttctg aatgttgtaa tatccgcatt
     2341 tgccggtttc aacatacatc atccatttta attcgtactt gccggttgtt ttgttgccaa
     2401 gtttcaggat caggtcggat gctgtcgagg aagtaggagc atcgcacaat actgattttg
     2461 ttcccgagtt ggcaaaggcc gtggaaattt caccatcttc cgatgagccc ggattgttgc
     2521 tccaggtggt ccaccaggtc gaattctggg cggctatgaa gccccctacc gtgtaagatt
     2581 caaaatcgtc attatatggt agggtttgtg cctgaagccc gaccaatgaa aagacgattg
     2641 ctgttaaaag cagagtaaat tttttcattg atttgaagtt tggtttaaat aagtttgacg
     2701 tatggcaaat atatataata tcattattgt aacagacaaa tttaatgaac ataatcgcag
     2761 aaagttttaa ccttttatca gttcctgatt atgaagatat ttctataatc cggttaatga
     2821 tggctgcctt tttgtttagg cctgacaatt atagagtttt ccctgccagg tatctctctt
     2881 ttccagttca ttttcaataa gtttctgaac ctgatcggtg cgaaggtttc cccagacttg
     2941 tccggactga aaccggggag gcgcttcgcc cgaaaacctt tcgatgacaa atgccggatt
     3001 gagcctttca ataaacctta tgataaaatc gatatactca tcaagcggga ataaattgaa
     3061 ccgttcggga ttttgcaaat attctttctc cagaagtgtg ttcctgataa tctggagttg
     3121 atggaatttg atggtattca gcggcagcag cgagagtatt tcggcttccg aaagcatgtc
     3181 ttcttcggtt tcgccgggca ggccgaaaat gatatgggcg cctgtgtgta ttccgtattc
     3241 agaggttttc cggatggcgg tcaccgattc gctgaaggta tgtctgcggt ttatcttttc
     3301 cagcgtttgg ttgaagcatg actcaacgcc gtactcaaca atgatgtaat attttcttgc
     3361 caggccggca aggtattcca gtttttcatc gtcaatacag tcagggcggg ttcctattac
     3421 caggccaata accccggggt aggagagagc ctcttcatac cgtcgcttga gttcaggaag
     3481 tgatgcatat gtgttggaat atgcctggaa atatgccagg taattcgcag ccctgcggta
     3541 ccggacccga tgaaatccga tgccctctga aatttgttgg gtgatgcttt tttgaggttg
     3601 gcaatacgat ggattaaaag cgttgttatt gcaataaatg cagccgccat atcctttatg
     3661 tccatcgcgg ttgggacagg tgaatccggc gtcaatagct actttctgca ccctgtgccc
     3721 gaaggtgcgg cgaaaatact cgctgtagga gttgaagcgt ttttggtgac cccaggggta
     3781 tatgatagct gacataggga aaaaagaaag gctgcatcgc ggggatacag cctttcaaaa
     3841 gtacgattat tagattattc agttgccttc tcgagccgtt tcatgatgga gaaaatgaaa
     3901 gaagccgaaa gcaggcagca gatgaccaga acactccaga aggcataaag cggaagtttt
     3961 tcccagaaca ttcccaaaac accgacgaga taattcccga tggctgtggc tgcaagccag
     4021 ccgccctgca tcataccctt atactgggga ggcgcaacct ttgccacaaa agataatccc
     4081 atagggctga ggaataattc ggcaatagtt agtacgaaat aggttcctat cagccagccg
     4141 ggattgacca gcacgtttga tacgccctga agatcggaag gagcaggcag gcccatcgac
     4201 gcaatgatca tgacgatgaa tcccaatgcg gcgatcagca tgccgaaacc aattttcttg
     4261 ggtgccgaag gctcttttcc tttcttcccg agaaaagcaa acaacgcaac cgaaaccggc
     4321 gtcagcaata caatgaataa aggattgaac tgctggaata tctggggggt aatcggtacc
     4381 aggccaatgg cgtctttcac tacgataaaa taatatagca acagcccggc caaacctgcg
     4441 gcggacagga taatgcccgt aagcggcttt ttctctccgc caaacagccc gatggttgcc
     4501 tgataaatac cataggtaaa caaaatgaaa ggcaggaggg ccagcagtga aaacatcatg
     4561 ttctgaacag gactgatcat actggttgta taatcccggg cgaagaatgt caggcagagg
     4621 ccattctgat ggaacgacat ccagaagaaa atgacgacaa agaacaccag caacagggcg
     4681 atgactcttt ctttaacctg tttcggagta agtacgacca cgtcggcatt attgccggaa
     4741 agtttggctt ccttgagttg ctgtttatgg gttttatcca cagatttcca ggttttcctg
     4801 aaaccgagga ataccgatac ggaaataata aggcttatac aagcgagggc aaatgcccag
     4861 ttatacgatt tggtaagaga ttttaccaca tacgctttgc cgaagttgga agcaaaaagt
     4921 tcgggatttg aactgtcgcc atacaatttc ggatcagtga tggatatctt gttaatcttg
     4981 tcaatgatgg cattttttgt cgcggcatcc tcgggccttt tactttcgag catcctgtat
     5041 tcctgaagcg acaggctatt ggaatctttc aacaaaccgg cattggtcag ttggtataaa
     5101 cctgcggatt tcaggttgaa aagggctttg tctttatctt cctggaaaat aacagcctgc
     5161 tttttcttgg ctttctcaat aacctgtacg ctgtccttgt gggttgccag cgcttttgtg
     5221 ttattcttga agatatcttc gaggaatacg gtcctgtcga caaccttatc attgagtttt
     5281 acatagttct ttggaacaga agcgtcatag gtgaaatgtt cctgcgcaag aatggcttta
     5341 ttgatgctgt tggcggcgct tggtgcaaaa aacgccccta tgttgatgaa catgtaaaaa
     5401 attgaaaaag caatgtcgcg cttgtttttg tatatgtctg cttcatataa attacctacc
     5461 agtgcctgga gattcccttt gaataagcct gtgcccatgg cgatcaatgc cagcgcgctg
     5521 aacatcatga tataaccgcc agtgttcggt tccgaaggcg cagctaaaag cagataccct
     5581 gtgaacatca ctcctatgcc cagcaggatt gtctttccgt agccgagtac tttatcggca
     5641 ataattccgc cgaacaatgg taaaaagtac accagtgcaa gaaaactacc ataaataata
     5701 ctggtagtgg acgaagtaag cccgaatttt gcctgcatat acagggtcag aatggcgagc
     5761 atggtataat aaccaaaacg ctcacccata ttggcaatag cgagtacgat taaccctttg
     5821 ggatgctgtt tgaacataag taactgattt ttagtgtata gtaattttat ttttcttctt
     5881 tgcgaagcaa atttaaccaa ataagcttga aaatgcccgg ttatcccgat gaaaagaaaa
     5941 ttaaagtttg gggagtcaaa cagagaggtt taaaaccgca ccttcatcat taccctgaca
     6001 tcactttttg tattgccgtc aatctgagaa atcccgcttg aaatttgctg ccggtcaaaa
     6061 taggtggtga cagaatacct cagccagata tctgctttcc ggaggattga aattttcagc
     6121 atggtataaa agcggctccc ttttcctgaa aaggatggca ccgaaaacga ataaggcata
     6181 tcgctttcgt aagcatagat tcgggagtcg tacgaatctg catcaaaaag ggcataacgc
     6241 aaaacagcgg aaaacggctt ttcttccggt cggaatatca gatcctgact gatgaagaat
     6301 cctctctcag gatttgtttc aggagtttcg cgatgtaaat aatacagccg gttgcggagc
     6361 gtcagccatg gcaatacctg aaagttcagc aatacctgac aatagttacg ggtaacatct
     6421 tcaacggagt ttatccggcc ctcttcacgg ctgaaattca acggagtgtg aacctgtctg
     6481 tacctcaggg taatatctcc ctttcgtcca aggctgtact gtatccgcac cgaatactca
     6541 tcgccatgcg agggtgcgtc aaccctgaac cggagccagt catagcggaa taaatcggaa
     6601 tatgcgacga tggcgacatt tctgaagggt gtggcattca atccgaaata taatcccttt
     6661 tcattggtat tatcggcgct ttcgccaaag gctgcagcat aggggtttgt gtaatctttt
     6721 ttataatttc ggtataccag cgacatcgaa aagcggggat caggcgacaa cgacaacccg
     6781 tttaccgtgg ctgtgccggc atgaagctgc tgcgccgttt ctccgaatat tgtcagccgt
     6841 ttgtatgagt aacaataatc aattcccagg aatatgcttt ccctgtctgt cttttcgaac
     6901 cggttataca gcgattgacg cggattatac aaatggttca gaagggaata atatccggtt
     6961 gcgccgatac gcaacctctg acctttataa ttcagatgtc cgccggcaac ctgttgattt
     7021 accgaagctt tgtcggaaag ttcgcccggt gtgcgatggt aaccggtttc gatgattgag
     7081 gtgaggtacg cctcggtccc tgcggctgta tctgcgtcaa agtagttggc gtcaatgttc
     7141 cggttggaat agaacccggt gatatcaacc ctgttgattg aaaatgttgc tgcagccccc
     7201 ctgaaatatg catactcgtt ggcagaggca tgcggactga ccaatggtgc acgtttcctc
     7261 atggcaatag ccccgggcga tttactgaac gacagccctg tccagagtgt gagcccctgc
     7321 ccgaactgga ggtggtagtc gccgatggcc aggtgcttta gcttgccgat atctctcagg
     7381 taaaaatgaa aagagtaata atcgaaccct ttatgcaggg tatcgctttt cggaaataag
     7441 gtttcaccgg catctttatc ggctacaagt cccagatgca tcctgtcttt ataggtatag
     7501 gtaaatcgca cataaaaggc gtcggggctg cctaggaaat acttttccgg gttttgggca
     7561 cgaatgctgt cggccacttt ggtaaagcct tgttgtttct caaaaacgcg ctgataacgg
     7621 agcatcagtt cggggcgtcc atatttaaaa gctcttttta tattgaattt ttcactgatc
     7681 gggtcggaaa gcgttatata aggcaacatt tgcagtataa atgtttcgtt gaagccgtca
     7741 atagcctgca gttcataaat gctggcaaat gcaccgtatt tccggtgata atccagaagg
     7801 ttgcagattt gctggtcgtt aagaaatgga agccggcgaa gttcctcttc ttcagttgaa
     7861 ttcaggttta tcggcctgaa ggacaatgac cggagttcat cggtccattc gctgaagtct
     7921 gtctccgaat cggtttgttc cgagatgtat tcaatcctgt cctgatgcag gtcattatcg
     7981 ggcggttgca atgaatcggg ttgctgggca gaaacgagtc cgggaatccc tgtcagcatg
     8041 atgaacccgg ttattccggc cgctttgata cgaagcatat ttcccgcgaa ccggatgtga
     8101 aatgcagaat agcgcatatc ggaatttttt aattacctga atgcatattg cagagaaacc
     8161 tgaggcgagt agcctagcac ttcgtgtata gaggaggaaa tatcgaaagt aaagtttttg
     8221 atttcgaggc cgaatccgaa tgtgtatttg aacggattgg tcgccagtcc gatacgggca
     8281 aagccttttt taaagaagcg gtattcggtt ccggccatga actggatggg ctgtaatgag
     8341 tttttactaa cctcggccga gagcagcagc gcttccgaaa aggtatacgt aaaaccggcc
     8401 ctcatgatag ccgggatacg ttcatcgcaa tgagcggaaa cttttctgct gagcgggtta
     8461 aaaacgtgag cgccgaacgt caggttgctg gtgagttttg ccaggatacc ggcttcgaat
     8521 gtagcaagac ctgaagaccc gtattcattg gaaagaccgg tatgaagata atcaagctga
     8581 atgccggcgg caaaatggtt gccgaaagcc cgagaatatg ctattcccac gtttgcctca
     8641 ttataaacgg cgtcgccggt ataggctaca tttcccccaa atacaccgta acgggtaggc
     8701 agcgtaaagg cggcagcctg gtaaccaagt tccttcaaca ggaagctgtt ttcataataa
     8761 aaagctgcaa cgggcctgtt gtaaaaggcc atgcctgcct ggttgttatg gatactccag
     8821 ctatcgggaa atgaaacaga aaccattccc atggcggcgg atcgggcccc ggcgggtctc
     8881 caatggtcac cggcacgggc agtgacaaat agcattaata ctgaaacaga aactaaaaaa
     8941 tatttcatat ataaaatttt gactaatata tgaaaaaaat ggaaaaagtg ttctccttgc
     9001 cgtaaatttg gcaggactga ataaaccaga tgattttcga taagaaagag aaactatgat
     9061 cacagcaaat acgcttgatt tcctgtcccg gctgaaggaa aataacaaca gggaatggtt
     9121 ccagcagaat aagaaacttt atgagcaggt aaggggcgga tatgaaaaat ttattgcgga
     9181 gttgattccc gacatcgcag tgctcgatcc gtcgataggc ttcccggatc taaaggattg
     9241 cctgttcaga atatatcgcg atgtgcgttt ttcgcccgac aaatcgccgt ataaatcgca
     9301 tttcggagcc tttatcggca aaggaggacg aaaaacgacc ggggccgggt attatgtaca
     9361 tgtggagccg ggaaacagcc tggttgcagg gggcatttat atgccccagc cggatattct
     9421 gaaactgata cgtaacgaaa tctatttcaa cagcagtgat ttcaaaaaaa ttataacaga
     9481 ggctgcattt gtgaatactt tcggacggct tgatgatttc gataaattaa aactgcctcc
     9541 caaggatttt ccgtccggtt tccccgacat tgacctgctg aaataccgga gctatgttgt
     9601 gggcaggcct ttgtccgatg aggaagtgct gagccccggc tataaaaaga atatactgga
     9661 aatattcaaa gccatgcttc catttcatga attcctgaac cgggctatcg ataaccggta
     9721 agcctcagca gcgctgacag aaataattaa acccggtata aaattgccgg gcttttaatt
     9781 ttcggagtaa tacaggttat tatgcgtcga tgttggcgta tcgggcattt ctttcaatga
     9841 attcgcggcg cgggggtact tcgtcgccca tcagcatgct gaagatgcgg tcggcttcgg
     9901 agccgttttc tatcgttacc tggcgcagtg tgcggaaatc gggattcatg gttgtagtcc
     9961 ataactgttc ggaattcatt tctcccaaac ctttataacg ctggatatgg attcctttct
    10021 ccgttccgtc tttcgaaatt tcattcacca gtgcgatccg ctgttcttcg gtccagcaat
    10081 atttttcggt attccctttt tttaccaggt acaggggagg agttgcaatg tacacatacc
    10141 cgttttcgat gagttccttc atgtaccgga aaaagaaagt aaggataagg gttgcgatgt
    10201 ggctcccgtc aacatcggca tcggtcatga tgacgatttt atgatagcgg agtttatcga
    10261 ggttcagggc tttggtatcg tcctcggtgc ctatggttac tcccaaagct gtgtatatat
    10321 tccggatttc ctcgctgtcg aaaattttgt gttgcatggc tttttcaacg ttgaggattt
    10381 ttcccctgag cggcaggatt gcctgaaact tccggtcacg gccctgtttg gcggttccgc
    10441 ctgccgaatc tccctccacg aggaacaact cacataagga agcgtccttt tcagagcaat
    10501 cggctaattt gccgggcagg ccggagcctg agagcacgct tttacgctga acgagttccc
    10561 ttgctttacg tgcggcatga cgagccgtag cggcaagaat caccttgttg acgatcatct
    10621 ttgcttcgcg ggggtgttct tcgaggtaat agttcagcat ttcagcgacc agttgatcca
    10681 cggcgcccat aacttcattg ttgccgagtt tggttttagt ctggccttca aactggggct
    10741 ccataacctt taccgagata atggctgtaa gtccttcgcg gaaatcgtcg cctgagatat
    10801 cgaattttat tttttcgagc atgcccgatt tttcggcata cgttttcagc gtgcgggtga
    10861 gcgcccggcg gaaaccggcc aggtgcgtgc cgccttcgtg ggtgttgata ttatttacgt
    10921 atgaatgaat attctccgaa aacgaagtat tgtactgcat ggcaagttcg accggaatgt
    10981 catttttctc accttccatg taaatgggct cggggatcag cttctcgcgg gtttcgtcga
    11041 ggtagttgat gaaatctacc agtccgttgt tcgagaaata ggattcggat acatctttgc
    11101 cgctttcgtc tttttccctt tcgtcgataa gggtaagttt gatacccctg ttcagataag
    11161 ccagctcgcg aagccgtgag gaaaggatgg tgaaatcgta atgatcggtg atgaagattg
    11221 aattatcggg tttgaaatgg acccgtgtac cggttacctg cgtcgtaccg acgattttta
    11281 cgtcgcaaag cggtttccct tttgaataat cctgttcgaa aatcttgccg tcacggtaaa
    11341 cggtgacaac aagctgatcg gaaagcgcat taacgcatga aacccctacg ccatgcaacc
    11401 ccccggagac tttataggac cctttatcaa atttcccccc ggcatgaaga acggtcatta
    11461 ctacctcaag cgcagatttc tgttctttct catgaaaatc ggtaggaatc ccgcgtccgt
    11521 tatcggtaac ggtaatagaa ttatcggcat gaatgacaac ttctattgta tcgcaaaaac
    11581 cggcaagggc ttcgtcaatg gaattatcaa caacttcata aacaagatgg tgaagccccc
    11641 tgacccctac gtcgccgata tacatggcag gacgcttgcg aacggcttcg agaccttcga
    11701 gcacctgtat gttttcggct gtgtaatttc cattcccgtt tccatttgat gagccattcg
    11761 gccccttagt tatttcgttc ataatcaata tgttgcagtc caagttttaa tatatcgcaa
    11821 aggtaggcat ttccctgtta agaacataga aaaccggtct ccgaaatcgg gttttttatc
    11881 aacaaatctg tacggttttt gaacatgcaa ctgacagtaa aacagtaata tatagaccga
    11941 aacgggaaga aaggcaaaaa tgacgtcccg gattcaaaac atattaaaaa ataatgatat
    12001 aggatagctt tatcaggaaa atatcctgcg gatgagtatc aaataattcc ttcatatttt
    12061 cgtggatatt gaagcggtga tcttcctcgt aaccggtccg tccctgcgac cagaccaggt
    12121 acaatactga gcctggctta tattcccagc gggcaaccag gttcgaacgg aactgcatga
    12181 acctgaaatc gggctcctgg aaattgtaat cttccgtacc atcggagttt tcgtcgatgg
    12241 agtagtagaa atcttcgtca gtttcactgt aataacgtga aatttcattg gctgcatatt
    12301 cccggaacct ggcataataa tcattggcgc gcggatcggt gacgtatttg taacggctgt
    12361 atttgcctga gaacaggaac ggctgtcccc agaactgaat ggagaaatcg ggcgtaacgc
    12421 tgatatttac cctggccgac aagctgaaga tttttttatc gaggcgcgcc aatacatacc
    12481 tctcctgtcc atcggacgaa ggaacggaat aattatatag cagatcgata tactgtaaat
    12541 catcatggcc ataagaatag cccggcatca gggttacgga aacagaattg acaggtttat
    12601 aaacgattcc gaaatcgatg ccattcgttc tgtaataatc acagaagccc ctgttcatgc
    12661 ttgcatcaaa ttcaaaaaac agctttttcc ggttatcggt actcagataa atgaaattgt
    12721 tgatatcgcc cggcatgaac aatgcagggc cgccccgcag gatggactgc gacagggagc
    12781 gtgaatctgc atttaccgaa aagttaaact gccagtaatt cttaaactgt gcgtttccgt
    12841 tgatattacc cccggcataa gtccattcac ccccgaaatt ccagccattc cactgattga
    12901 aattgatacc aagatacctg aaaatgctga acggcttgta aatccggtaa cctacccagg
    12961 cgacctgctg aatttcatcc gcccgtctca tatacccgac atcgttaaaa tcgaggcccg
    13021 gcgaacggaa ggttaaccag cccatatact gccagtggcc ctgtccggct tttccaccca
    13081 taatggttcc tccatagccg ttcaacgtgg tgatcgaagt atccatcttc atataatcct
    13141 ggtcaggttg ctggaaatag tgagccgacg actgctgaag ttgggtgata cattcacggg
    13201 tgccgctcag cgaactgaat acgcctttca gcatgagata gtaagttttg tttttccagt
    13261 agcgggtata atcaacgccg ccggtatagg cgctccgggg aaggaactcc aggttgctgt
    13321 cgttgatatc gcggttggtt gccgtgaaca tgcctcccag tatggtttcg cctttgctga
    13381 aatcgcgttg caggcgggct acggtgtaat tggtaaaagg ctccacggtt actttacgcc
    13441 ggtcaccgtt ccgatcgatt gaggcccttt cactttgggt aatggattca agaataccga
    13501 tggacaaccc cttatttgtc ttgcccgata gtttgaaaga gccgagaatg tttgtggagg
    13561 caggaacatc ggcgtactca tcgtccagca ggttatcata aagatcattg ttatactgcg
    13621 gctgacggcc gatccggcgc gagtaaaaca acatgttgcg ggtatcgtcg ccgtcgccgc
    13681 cggtcaggct gaaatcaaaa atgttttttc cctcaataaa aaacgggcgt ttttcggaaa
    13741 aataagtttc aaacgccgtg aggttcacct ccgacgggtc ggcttctacc tgcccgaaat
    13801 cggggtatac cgtgaaattc agcgtcagat cattggtaat tcctatttta ccgtcaacac
    13861 cgacttttgg cgtgaatttt ttaccggtgg cgaaaggatt tccctcctct ttttctgata
    13921 attcttccga cgccatcaga tagggcaata tttcgatgtc ccggtgaggt tttaatccgc
    13981 tgattccctg cagttcaccg aaaaggctga cgaaccccgg agcgtttctg gccaccggct
    14041 gccacaacga catttcctgt ttccggtata tataacggaa aacatcaagc ccccagttgt
    14101 gttcttcttt ggatgaaaaa cggatctgcg acagcggaat cctgaattct gccatccagc
    14161 cttgtgaatc gacggatgtt ttggcatcaa aaatggcatc ccaggtttca tcgctgttcc
    14221 cgtcgttgat caccatcagg tcgaacttca cgccggctgc cgtcactccg aacccgaagc
    14281 ctgttagttt atcggcgtaa ctgtcgatga tgatccctac ccaatcgccg tctgaattat
    14341 cccgtcgcga tagccggcgg acgatttttc ccggaacgga gtcataagcc cggacggcaa
    14401 tgtaaaggtt gttgtcatca tataataatt tgaaagctgt ttgctgcgac ggggatgctc
    14461 cgtcatcggg ttcgcgctgt acaaaatttc cctgccattc cgcgccattc cattcgtcat
    14521 cgtttatcac cccgtcaatc acaggggctt ttaactgaat ccgggaagtc ctgtaaattt
    14581 ttacagacgg gttcggctga gctgagaggc cattacagat ggccagaaaa attaatggaa
    14641 ggtatagaaa ggctgtaatc cgttgcataa gcgaagggta tagagtcacc ccctgacgtt
    14701 tttgattgta cgacggaaac cagggccttt atgttacaga ggaattcctg aaaatatgca
    14761 ggaatttgca taagtaattc agggcttgtt gtttgtgaaa accttaaacc gggacagatg
    14821 ttaacgatat tgtttgccga aatccggttt gtcagaatga aaagcttacc ccgcccagca
    14881 cattaaaacg gtaggaagga tagttgtacc aaagccaatg gcgggtattg gtgaaattgt
    14941 tgaaattcag ccagaaagag agcgctttat tgaatcggta ttcaataccc aggctgccat
    15001 cgaaccaccc atcgagtttt tcggatattt tccccgatgt acccggcgtt tcatccggct
    15061 cagatgtcgc aggccgcaat gcccataccg gcccgttata atcggcctgc agtttcagta
    15121 ttatcttatc ctgaatgtta taccggctgt tgaacccgat cacaaattcg ggtttgtacc
    15181 agggatgttt ttcataaaga agcttataat acctgttgaa tgctcctgag agggcgagat
    15241 ttaacttttc ggatttgacg tattcaagtt cagccctgag tttcataaca ctgacatcgt
    15301 catattttaa catgaaggtg ttgttgaggc tggaggtttc gccgacaaaa aacgggttgt
    15361 tttcgaccga actggctgaa atgctgccgt tcaaattgaa actcttgctg atattgctgc
    15421 tgaatccgcc gtaagccctg aatttttcgt tgacatacct gaattcaagg ctgtccttga
    15481 taaaagggtt ttcgcaggcg gtttctttta atgtatggcg gtgcatcgat ccgtcgatgc
    15541 ccgcataaat ctgcagggca ttggggattg cttcgagccg tgcttcgagg tatggataca
    15601 aatggccttt ggtgaccgta tcgccctgaa cataaaaact gaatccggct ttaaagctgt
    15661 attcgttgta ctttaccccg ataaaaggct gcaacatcac aactacgctg tttgttgttt
    15721 caaagaagcg gttcctctgg ttgtaaaact gaacatcggc agtgagtccc agtgtttgct
    15781 tttctttgag gttaaacaaa tcggctgctt tatcggctgc ggcattgaaa tgaatattgt
    15841 tttcgctggc gtcgaaatta tcttccagat aagagtaacc gagcgatact gcatggttaa
    15901 gtttgttgtt tcctttgtaa gcgctccgga aggtaagatt gccgccggca ctgatatatc
    15961 gttgtttgat gctgtctttt tcaatatcga acttttccag cgtcgagtcc ggcacaccgt
    16021 aaaagtgtat tgcatcacgg ttgaaataag cttcggcgcc aagaacgtga tctgaaaaat
    16081 aacgttcccc ggtgagatca gccagttggt gcgagttggc cggagggcca aagtctttga
    16141 tagtcccggc cgatgaaagg tgttttatcc ttaccccgac catgcccttt ttcgaacgca
    16201 aagaagaggc attcagctca ccgtaacatg tcgcataatt tccgaacccg ccccgcaaat
    16261 aattccggta cagtttgttg atcggctcgg ctaccaattt gggcgaaggt aaaggctcga
    16321 tcgccagttg aatagttgcc ggtctcggct gaatattata cgtcataacc ggaatggtgg
    16381 tggaggtgtc gttgtttacc ggattctggt ttattttaaa agcatcgggg atttctggct
    16441 tataagtagc gataacggtt atctcctccg tgtatttggg ttgttgggca gaaaccaata
    16501 ccggtatcat tgccatgatc gcaacggcga gccgcagttt cgttactttg ctgtattgcc
    16561 tgttcggcat atctaagcgg ggtgatatat tcatggtgtt tgatttctga gaatgattac
    16621 tgattcaccg gggaacttat ttcggtttcg ttgatagcat taagcttttc ccgggctgta
    16681 acaacaaggt cttggccttc gtagttatcg ataatactct ggagcgtttg ttttgcctgg
    16741 aaagtgttgt tctttttcac atacacgtcg gcgagcagga taaagccttt tgccacccaa
    16801 taatcgtatg atgcaaaatc ttcggccagg gcaaaaagtg ttttttcggc ctggtcgtaa
    16861 gcagcagcgt cgtattgaag ttgtgcggtc ataaattttg cttcggctcc catttcgttt
    16921 tgagaaagtt tcagggtttc ttcaaattct ttccgggcca gttcgctgtt tcccaacgca
    16981 taagccgaac ggccaatgta caggtgcgct tcaacagcca ggttctgttg caggttttcc
    17041 ttcaataata gtttttgtcc tgcctggatg gccaggccat atttccccag aaggtaattg
    17101 cactgcatct ggcctgtaat cgcatcggta acatgaatgg gctgttcgga aatttcttcg
    17161 agacggataa aattctcaag cgcttcggag taatctttct gtgcatactg aatacgggcg
    17221 gcttttaatg ctgccgttgt cgaaaatgca gtccgctggt tattcaggac aaacagatac
    17281 cctttcaggg cctggtcata cttttcagcg cggtaatcgc attccgcttt ataaaagttg
    17341 gctttgatgg taaatgaacc ttccgggaaa cgggccaggt atttatcgag actttcggtg
    17401 gctttttcac agttgccgtt catgtattgg ttctcggctg ctataaaagt gagcgaatcc
    17461 tgctcggtgc gggatacatc ggcctgcggg acagttttcg tgaaatcaac gtactcctcc
    17521 accttgttct ggtctacata aatgctccgg atgctggcca gcgcctctct cgattcgggt
    17581 gttcccggat attcctttac tacgcgtttt aaagttgcca gcgcatcgtt gtcgcggttc
    17641 tgattgaaat agatcagtcc tgtcttcagc aacgatttct ttacataact gcttttcggg
    17701 taatcgttga caactttttc gaaatagctc agggcttctt cgttccggtt gatgatcgta
    17761 taggtgttgc cggtttcata ataggcatca tcaaaatagg ttgaccttgg gtagtccttt
    17821 aatagttttt gcaaccagtc gattttttgc tgcatttctc cctgcacgcc ataagccgta
    17881 gctatctggt aaacggcaaa atcggcatcg ggaaccttca gggtaaccgc cttttgataa
    17941 tatgtgatgg ctgtaccata atccttttgc atgaaataac aatcgccgag cctcaggttg
    18001 gcatctgcaa cgagttttgt ctcaacgggc ttacccgtga gatattttct gaaatgcagg
    18061 gccgcgttgc cgtattcctg ctgtttgaaa taacagtaac cgacattgta attggcggtg
    18121 ttgaaaaagg gctgtgccga agcgccaggg ctcaccagaa atttgttgaa aacttcggcg
    18181 gctttgtccc actcgccgag gcgatagtac gattcagcca tccaataagc ggaagccgcc
    18241 tgaacctgtt tatcactgtt ggtttcctgc gcctgcttaa aaaggccgat ggcgccgtta
    18301 tagtcggtgc tgctgaactg ctcgatagcc ctgtaatagg atattttctg gtaagcggca
    18361 tccagccttg cattacgctt tttaatctgc tgaagggatg tcagcgcatc cttgtaattc
    18421 ctcgtgattg tgtataagtc ggcaaggtag ccataggctt catcgagacg ggaagaagcc
    18481 gggtactcat tgatatattg ctgtaaagct tttactgctt cattgtatgg attataggat
    18541 aattcgatgg ataatttcgc atagttgaac agcgcatcct cggcaagggc cggatcggcc
    18601 ttgatcttac aggcagaaag aaaagaattg tgcgcatacc gcttctgacc ggttttcagg
    18661 taactgttgc ccaggtggta gtatgcattt tgggataacg aatcctggtt ggttgccaca
    18721 agctggaaat atttcaccgc tttgttaaaa tcgccgttat agtaagccgt aaaggcaatt
    18781 tcataataat cgtttcgcga aatatttgcc gggttcgcat taaggtaacg gtcgaaacag
    18841 gaggcggctt cctggtagtt ttcaatatga taataggcat cggcagtgag gcgggccatc
    18901 tcaatgttca tcctggtgtt tccctgttgt tccagcaaag ggacggaacg ggccagcagt
    18961 tcattatatt taccctgcat ggagtaaatc tgaacaatat aataatcgac gacgtccttg
    19021 aatgtttcgt cgttttctat cttctcaaaa tgtttcagcg cggtttcata gtttttctca
    19081 agataggcga tgctggcgta atagaaattg gccggggcat ggtaacggtt gggcttatct
    19141 ttcactttgg ccagttcttc ttttgccgtg gccaggttat cggttttaaa agcagagtac
    19201 cccattttaa aatggtattc atcagcctgt tcctggccca gttcataaat atcgaccagt
    19261 gcaaatttca gcatggcgtt ccggtagtcc ctgctgttga aaaactgttt tcccagttca
    19321 aaaaacgcat tattcacacg ggcatgttcc gggtattttt gaataaaagt ctcaagccgt
    19381 tctgtggcat caggatgaaa aagttggccg gcacataagg cggcataata ttgagcgcct
    19441 gcgctgaggt tatcattgcc catggaggcg atccggtcga atatggccag cgcggaacca
    19501 tattttgccc tggaatataa atcgacggca ttgttgtatt ctgcctgggg ccacctgtat
    19561 acctgcgttt gctgggcctg agccccgaaa accgtcagga gtgtaaggag gaagaagatt
    19621 accggaaaat gcttattcat agaatggttc taagatcaga atttaaaatt acggctttaa
    19681 ttccgggcaa ctaatgccac ccggagcgat ttatcaacac gccgtgttta acaattttaa
    19741 cgtaaaactg tcgcaggtag tatcgacgca acaggatgat gttcccaaat aatcagggat
    19801 cagcgctgca ttcagggcgt gggcagggat gccgttaatt aacgatagtg atctttcggg
    19861 tatctgtcac gccgttgccg tcaataatcc ggagaacata gatgcctgtt ttgagcgagg
    19921 aaatatcaat gaattcggtg gaatgcgaca tgcttctctt ttcttcagcg agcaacgcgc
    19981 cgtttattga aaataatctg attgtcaaag gtctggattc gctgaaggat gtttcaagga
    20041 taagttgcct tgtctggcca ttggcataca cccggtatcc gttgccggaa gtaacccctt
    20101 caattcccac ggggccatac aaaaacgaaa gattatcaac ccacattttg gttccggtat
    20161 gagtctggcc gtccaggata tttgaacata agaacaggat gttcatggta tcgggggctt
    20221 cccaaatccc gtattctatg ggtatttcga agtatgtcca gtctgataat tcttcggcta
    20281 tgtattggta ggcaaagcca atagtgtcgc gtgttccgtt ttgccatttg ctgagggccc
    20341 aaccgaaggc acacatgtcg ttgtttacgg gttgatattt tacataaccc gacaatttgt
    20401 ttggcatacc tgtaaagggg tagcctcccg ataaattata agtctgattg tacacgtcaa
    20461 cgctcatttc tgccgtgctc agcacgcccg ggagcgaata ggttccgaca aaaggaacgg
    20521 ttacggtgac tacctgaagc aatgcggaat aagcgccttc ctgcggattg tcagattcct
    20581 ttaaaacggt attaaaaaaa ggctgattgg ttgtatgcca gtctacaggc tcactgttgt
    20641 tccactcttc aaagcccgcg ttgggaatgt cctgagctgc ggctaatgaa atgcagaata
    20701 taaagataat cagggtgctg aataattttc ggtatgattt catagaaggt gtaaataaag
    20761 taacatccaa aagtaataaa aatcccctgt taccagggga ttgacaacaa aaactgcaac
    20821 gatcctgtaa gcgggattct gttccggcct cgtctgttgc cggacggccg gtatttgtca
    20881 tttatctggg attgccgtcg ccggcaacct ctaacggccc accccccggg tgtcgggcga
    20941 gcaacccttt tctgccagcc tgccgggcag gccggcagaa gcccggtata tttggccttt
    21001 caatccataa ggtttaccat cgcgcctgtc gccaggacac gtcgtgagct cttacctcgc
    21061 gttttcaccc ttatcccggt tttcaccgtg acggtatatt ttctgtggca ctatctgttt
    21121 ccacggtgtt cagccgcaga accttcccgt taggaagtat ggcgctctgt attgccccga
    21181 ctttcctcat ccgcctgaag cggacgcgac aaatcggatc gttgccaatg caaagttaca
    21241 aataattgtg gcgccgggcc cctgatctgc tgcataaatg ccttgacgga aactagcccg
    21301 ctttaccaaa cggtaatgac ggggtatcgg cagctaccgg catcaggatt cctgcagccc
    21361 cgccagctga actctcaaag cgcgcaaata ccggaaatta ttgattttgt tacctgataa
    21421 tggtcacagt ccctttgatc gtccgtttga cgattcctgc taatctgatt tcatagacat
    21481 cacaaacata gtaatatact ccgtcgctgg ccggctggcc ggtattcagg attttcccgt
    21541 cccattttat tgccgggtct tcggttttaa ataccagcct tccccagcgg ttgtatatct
    21601 ccaggtgaat tttttcaacc gaagaatagg ggaaaggaac aagataatcg ttgtaggtgt
    21661 cgccattcgg ggtgaaaaca ttgggaagcc tgtatccgcg gcaggtgtcg ctgtcgatgc
    21721 acagagtgtc gctcatagcg ctgacattgc ctgccgaatc aaaggctgca actgcataac
    21781 accctgcaat agtggatagc ggcccgtgca ggaatgtggt attccctgct ccgtcaaccg
    21841 aatctatcaa cgagagctgt tctgtttcag gcggccggta ccagatgtaa tacccggcga
    21901 tatcggacga acaggccgga tcgggatttg accacgataa cgtattgata aaggtgttgc
    21961 agtctgtttt aagaaccggg acaggagggc agggaggctc gttgtcaatc ggaatgccgc
    22021 aggtttcctg cgaccagttc agcaggggat cggtgatgcc ctcagtaccg taactcccga
    22081 tgctttttac tttatagcag taggtttcac cgttgatcag atcgcggtcg atataatacg
    22141 aaaggtccgt tgtgcctatc gaatcaaata aatgcgtatc attattcagg cggtaaacaa
    22201 taaactgcga gttgagccac gacgtctgat aattgaaagt tatcctgagc ttttcatcgc
    22261 cgggagcagt ctgaatgaaa acggatgacg ctttttgtga agcgccgatc aggaatctta
    22321 atcccggcgt gtcgttataa gtatcaaccc ggtaacttac ggcatcatcc gaagtattta
    22381 caggatggtc gatataaatg gtatcatcca gagatgacag tgagtcgatg agcgcgaggt
    22441 tgctcccggt cagtccgtcg gatctgtaaa tcaaatattt ataaggcccg ggcgcctgaa
    22501 cagggtcaat ttcggtaggt ttcgaccagg caaggtacac ctggccatcg gttaccgatg
    22561 ttttgtcgat gctgacattg gtaataatgg cgacatcttt tttgagtttg gcgcacgctt
    22621 catcggatgc aatactctct gatccatctg gataaacggc tgtaacgatg tagcagtaat
    22681 ccactccgtg aaccaacccc gcgcctttgt cgtcgtcgat atacagcgta tttccccagc
    22741 cgttgacgga tgcaaccttc ctgtaaccgg ttgaggatgg tacgcccgtt tcgcaatagc
    22801 ccggaacaaa tccgtaaaag ccgtttctcc ggtatatatt gtaactcacg gcatccgggc
    22861 atccgttggg gctccatgac acattaatgg aatttcccac aggtacagcg ttgatattaa
    22921 gtgttttagg gcctatcacg cttatattta aggtatagat gtcgaatagt gatacgggac
    22981 gggcattatc tattgcttta atatagatct ggtaaggagt gcgtttaata tggctgcatg
    23041 ctgtgcgcca ttgcagcagg cctgttgcca gtccaaggct gtcggagctt tgttcaaata
    23101 cggcagggct ttctgccagt atgaacggct gtcctgtcgc tgttaatgca attttatcaa
    23161 ggtcagtatc ggtcgctgat actgcatagg ttagcgaatc gcccgctatt acgcaggtat
    23221 ccatcagagg ttttatgact ggtgcttcat ccacgcaggc caacaccctg atttgcatat
    23281 cacgggtaac gtaacctatc cggatgccgt tccgccattc ttcaatctga aaagcaaaat
    23341 tatattcacc gagcatggtt ggtttatccc acaggatatt tccggttgcc gggtcaatgg
    23401 tgaaagtact cggatttgcc tgatcgactt cgttcggcat cttgaatccc ggtatcggtt
    23461 cccctccttc gccgcggcat acgacaaatt tataggaaag gctgtctccg tcagggtcgt
    23521 aagctcccgc attatgcagg taaggatgcc cgacgcatgc tttatcgacg ggcggcaatg
    23581 taagcagggg cgaattattg atcccaacaa agggattcac gacaagttct gtttcgagga
    23641 aaaacgggac atccacagag ttgggtatgt tcatgattcc tgcattcctg ttggggtctt
    23701 ccagcgtaat aagataggtg gccgggcctg cataggtatg ttgcccggtg taaatatttt
    23761 tccggattga atcagaggaa ggcccgataa tctcgccaat atggtccgaa gggtatccgg
    23821 ccggagtggt gccggcaggg ccgttggtcc gcggcaggct tgaggacatc ccgtcgcccc
    23881 atttcaatgg aagttcgttg cggtcggcaa ggctgtatgc ctgggtataa gtaattatgg
    23941 tgacttcgta ggtaagatcg ccgatccgtc tataggtaat ttcaccggca cgctgatgcg
    24001 tggcccacgc agggacaaca atcagggcaa gtgaaatgag gataaaaaat cgtttaatca
    24061 taatccattc accgcgcagg gttttggtga agcacggata aaaccgattt caaaggtact
    24121 aaagtttatg agcactagcc gaaactgtat ttccaaatta tcgctgaaaa gaatatcctg
    24181 atgaaggcag gatttgtgtc ccgggctttt ccgggaggtc ggattcataa catcattttc
    24241 atttaacact gcctgtgcaa gattctgtaa ctttgcattt gtcaagatat acttgtgatc
    24301 tatgtatgaa gctgacgttg ataaggaaaa caaagagata atcaagcgtt accggagttt
    24361 actccgggcc tggaattcca acaagggagt acgcgataaa aacagggtga aggaagcttt
    24421 tcaggtggca agcgaagccc atcgcaccat gcggcggctg agcggagaac cgtatatcta
    24481 tcatccgata gaagttgcgc ggattgtcgc cggcgaaatc gggctgggtg aaacagccat
    24541 tatatgttcc ctgctccacg atgtggttga agacaacgaa gattattcca tcaaatttat
    24601 cgaggataaa ttcggggtca gggtagcata cattgtcgac gggcttacca agatcaagga
    24661 cggatatgaa accatggaat cgtcgcttca agccgagaat tttaaaaaaa tgctcctgac
    24721 gctttccgac gatgtacggg ttatcctgat aaaactggcc gaccggcttc ataacatgcg
    24781 caccctcgat gccatgcctg cccggaaaca aaagaatatc gccgccgaaa cattgtatct
    24841 gtatgcgccg cttgctcacc ggctgggttt gtatgccatc aaaagcgaac tggaagatct
    24901 ggccctgaaa tataccgaac cggaagttta caacacgctg gcccggaaac ttcacgacac
    24961 ggaatacgaa cgttcgcgct tcattcagag gtttattctc ccgatcaaga aatcgctggc
    25021 agctaatggc atttcgtttg agatcgacag ccgcgaaaaa tcgatttatt ccatctggaa
    25081 aaagatgcag accaaggaaa ttcctttcga agaggtatac gatttgtttg ccgtccgcat
    25141 cattatcgat tcaccccccg aagctgaaaa aataaactgc tggcaggtat acagcatcat
    25201 aaccgatcat tacagcccca agcaaaaccg cttgcgcgac tggatatcaa cgcccaaagc
    25261 caacggttat gagtcgctgc acaccaccgt catgagtcag accgggcaat gggtcgaagt
    25321 acagatacgc acccggcgta tgcatgaaat tgccgaacgc ggctatgcag cccactggaa
    25381 atacaaacag ttgaacgaac gcgaacgaag ccaggaaaca gggctagatc agtggctgaa
    25441 caaggtgcgt gaaattctca ggacgcccga ttcaaacgcg ctgaatttcc tggatgacat
    25501 caaacttacc ctcttcagcg acgaggtttt tgtgtttacc ccgaggggcg aaatcaaatc
    25561 attgcctgct ggaagcactg tgctcgattt cgcatataat attcatacac aattaggcaa
    25621 ttcctgcatt gccgccaaag tcaatcacaa gctggttcct ttgaattata cgctgaagag
    25681 cggcgaccag gttgagatta tcgcttcgaa gaaaggtatg ccaaaggagg aatggctgaa
    25741 ttacgcctat actgtcaggg ccaaaaccta tattaaagaa acgcttaaag agcaacgcaa
    25801 acagtttgtt gaagagggcc gtaaccaact cgaatcatat ttcaaggaac tcaaactgcg
    25861 ttttaccagg gccaacattg caaggtttat ggcctattat ggactggcga gccatatcga
    25921 cctgtattac aaagtagcca aaaacgatat cagcctcaac gatgtacagg attgtttcct
    25981 tgatcataaa gccgatctgc gggaagctgc tcccgaaacc ggggaaacgc aagttgtgcc
    26041 tgaaatacca ttatcaagaa tgctgcccgc tattccagac cttgacgata ccgattttac
    26101 ggtcagcaaa tgctgctacc cgcttccggg cgacgatatt gtttgctatg tttcgcccga
    26161 cgggtttatt caggtgcatc gcactcattg ccctgaagca atagaactca tgtcgcgcca
    26221 tgccaaacgt atgatttcac ccaggtggcc caatcgccat ctcaacacct accaggccgg
    26281 aatcaaagca accggtatcg accgtaaagg gcttattttc gaacttattc atgtaatcag
    26341 cgaagagatg catttcaatg tcacgttgtt ccatatcgaa acatcgggcg atatcttcga
    26401 agccgtcata agcctttttg ttcatgatac catcgatatc aaccggttga tcggcgaaat
    26461 aaaaaagatc agcggcatta ccaacgtttc gaggatcatg aaatttgaaa agttctcctg
    26521 atcctggaaa aatccggcca ttattttacc tgatttttta aaaccccggc aaaaatcccc
    26581 tcctgaaatc cccgctaccg cgcgaatcct ttcgcgtggt nnnnnnnnnn nnnnnnnnnn
    26641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26701 nnnnnnnnnn nnnnnnnnnn tccacaaccc cgctaccgcg cgaatccttt cgcgtggtta
    26761 gaataaatcc acaacaatta cattatctag caagccttta ttcccgctat agtccctggc
    26821 actactatat aaataatctt ccggcttgaa gaccaaacct tcttcaaccg gattattatg
    26881 aatatatctt atcttttcaa tcagtacttt attgctccat aattctaccg gctgattatc
    26941 atgcctccag aattgatgat gttttacatt agaactttgg gctgctgccc ttgcaaattg
    27001 ctcaagtaac cagtaccttc ggctttcttt aggattaccc attatagctt gcacaatagc
    27061 tctgctggta aaccgtttaa aatcacctaa taaatcctct ggcttattat ccccggcact
    27121 tctgaataca aggtgcacat gatttgtcat gatgcaccag gcaaaaactt ccaaaccttt
    27181 attcttttgg caaaaagcga ggctctccag cagtatttcc ttgtattcat ttctgataaa
    27241 cacatcaatc cattcaacaa cagcgaaact aacaaaatag actccttcag gattatgaaa
    27301 tttataattg cgactcattc aacaaaatta gcaaagatct aataccgcgc gaatcctttc
    27361 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcagtagc ggtggtgaga
    27481 aaagttccaa cggtggaggg gttctattgc cacgcgaggc gaatcctttc gcgcagtagc
    27541 ggtggtgaga aaagttccaa cggtggaggg gttctattgc cacgcgaaag gattcgcgcg
    27601 gcagcggggg ggcggaagcg gttgtgttga ggaaagttct cggggtagcg gggttctatt
    27661 accatgcgaa agcatttcag cctccggcgg agagaaaagt tcgcgcggca gcggggatcg
    27721 gcgaaataaa aaagatcagc ggcatcacca acgtttcaag gatcatgaaa tttgaaaagt
    27781 tctcctgatc ctggaaaaat ccggccatta ttttacctga ctttataaaa ccttggcaaa
    27841 aatcccctcc tgaaatcccg gagggatgct tatatataat tgattattaa taaaatatgg
    27901 cagcagtcgg atggtgtcta tttttattta taccaaatcc agataattaa ttttttttgt
    27961 acttttatga aaaatttatt tgatcatgtc ggacatgaac gaacgcgtaa attttgatac
    28021 ggtaaaaaaa atttttaccg attatctgga aaaactgggg caccgcaaga cgcctgaacg
    28081 gtttacgatt ctgaaggaga tctataacac cgacagccat ttcgacattg aaaccctgta
    28141 catccagatg aaaaacaaca aatacagggt aagccgcgct acgctctata atactatcga
    28201 ccttcttctc aactgcaacc tggttaccaa gcaccagttc cggaacaact gcgcccaatt
    28261 cgaaaaatcc ttcaaattcc agcagcacga ccactttatt aacctcgata ccggcgaagt
    28321 acttgaattc tgcgatccgc ggattcatga aatacttctg tcggttgaaa aatcgctcgg
    28381 ggtgaagatt gagcatcatg cgctgacctt ttacggccat aaggccggca gcgacgataa
    28441 agttgcgacg gttaaaaagg attgacttta ataagccggc cgctcggtta attctgtacc
    28501 tttacacatc gaaaccaggg cccgccgggt cccataattt catgtccata cgatgaaaat
    28561 agatgtattg cttggccttc aatggggtga cgaaggcaag ggaaaaattg tcgatgtcct
    28621 tactccgcgt tacgatataa ttgcccgttt ccagggaggc cccaatgccg gccatacgtt
    28681 agagattggc ggcgaccggc acgtcctcca tactattccc tcgggtattt ttcatgccga
    28741 taagataaac gtgataggca atggcgtcat cgtcgatccg ttaatactgc ataacgaaat
    28801 ccttgccctt cgggccaaag gtgtcgatcc ccggctcaac ctgcgcatca gcaaacgggc
    28861 gcatctcata cttccgaccc atcgtttgct ggatgccgct tatgaatcag ccatgggcaa
    28921 aggcaaaata ggttcaaccc ttaaagggat cgggccatgt tatacggata aaacagcccg
    28981 tttcgggatt cgctgcggcg atatccttgc cggcgatttc ctgaaaaaat ataaaacgct
    29041 taaagacagg catctgcgca tcctgaaatc ctataatttc gaccttgcga aacaggaaat
    29101 agataaaatg ccggtcgacg aatatgaaaa acactggttc gatgctgttg aatcgcttaa
    29161 agagttcgaa ctgatcgaca gtgagttgtt tatcaatcat gccttatcgg aaggcaaacg
    29221 cattctggcc gaaggcgcgc agggcagcct cctcgacgtc gatttcggtt cctacccgtt
    29281 tgtcacctct tccaacacca tcactgccgg agtatgttcg gggctgggcg ttccgcctac
    29341 agccatcgga aaagtatacg gcgtctttaa ggcatactgc acccgggtgg gcagcggtcc
    29401 gtttcctacc gagttatcgg atgaaaccgg tgaattgctg cgtaccaacg ggaacgaatt
    29461 cggttcaacg accggccgcc cgcgccgctg cggctggctc gacctgccgg cattgaacta
    29521 tgccatcatg ctcaacggcg tcaccgacct ggtgatgatg aaagccgatg tgatgaacga
    29581 tttcgataag gtcaaaattt gcactgccta taaaattgag gggatggaaa atcccgagct
    29641 aacctttgaa acgctgagcg gtaatgtaaa accggtgtac cggatttttg aaggctggaa
    29701 gacctcgctt gaaggattga ccaatttcgg ggctttgccg gcaaaactgt cggaatatat
    29761 ccggtttatc gagaaatccg ttaaccttaa tattgatatc ctttccgccg gccccggcag
    29821 gcaccagact atcatccggt aaacatcttt tattatttgg gccccggctt gttccggaga
    29881 tgttcagggc tccggtagcc ggttggcaga agggtcgtgc catatcaccg aaagatagcg
    29941 gcgaagtgtc gaaaagactt tttcggttat ttcagatttt ttatcattgc cgggtaattt
    30001 aacatgaacc tgcatggcaa agtctttagg taagctggta aaatttttat ttctgacggc
    30061 ggttctggtg atgaatgccg gtcatgccca atccggaatt gttgccggtg atacgactat
    30121 caggacaagg aaagtaattg aggcgctgag gattgaatca gccccgaaaa ttgacggtta
    30181 ccttgatgaa cttttctggc aaaaccttcc ggtggcccgc gattttactg agtacgcccc
    30241 ggtaaacggc atcaagccgc ccttcaggac cgaagtccgt tttggtttcg acgatattgc
    30301 attttatgct tccgccgtga tgtacgatcc gcatcccgac agcatttgcc gcgaaatggg
    30361 gaaacgcgac cagatagaac tgcttaatac cgattatatc tcctttgaca ttctgcctta
    30421 taatgatgcc ctgaatatgt atgaattcaa aatcagtccg atagggttgc agaacgatac
    30481 caagtattca gcgctgggac aagatgttaa ctgggatgcg gtatgggaat cggcggccag
    30541 gatcaccgat tcggcctgga tcgtagaagt gaaaattcca ttctctgcgt tgcgattttc
    30601 aactgccgaa aagcaggttt ggggtatcaa tatgtggaga aacctgaaac gcaggaacga
    30661 atggtccacc tggtgttacg tggataatac cgttcaggat gatcttttca aatattacgg
    30721 cgaagttgcc ggtatgcaaa atgtcgatcc gccattgcgc ctgtcggtta cgccgtacat
    30781 tgccggttat gttgaaaaaa atccatcgag gcagcaatgg tcttcgctgg ccaggggcgg
    30841 catggatctc cggctcggga tcaacgaaag ctatactgtc gatatgatgg tgatccccga
    30901 tttcggtcag gtgcaaagcg atgaccgggt actgaatttg tctccctttg agatccgtta
    30961 cgatgaaaaa cggcagtttt tcaccgaaag caccgagatg tttgaaaaat gcggtatttt
    31021 ttattcaagg cgggtgggaa gcacgcccag gaattttaat tcggcgtatg atcaggcgga
    31081 acaggatgaa accgttaaaa tgaaccccga ccaggcccgt attgttaatg ccacaaaaat
    31141 ttcgggcaga aacgccaatg ggcttggcgc cggattgttt aatgccatga ccgccaatac
    31201 ctgggcaacc ttggaaaata ccgaaaccgg ttcaacgcgc aaagtgctga cgcagccttt
    31261 taccaattat aacgttttgg tgctcgacca aaacctcagg aacaaatcct atgtttcgct
    31321 gataaatacc aattattaca gccctaagga cagctatgcc gccaatgtta cgggagtaga
    31381 atcaaggtta agcaataaaa agagcacctt cgtctttttt gggaagctga atgtcagcaa
    31441 caagattcaa aaaggcctga aaacagctac cggccatgct atggaaatag atatttcaaa
    31501 gccgaatgga aagttccagt attatctggt ccgtgcacag gctgataaag tatatgatcc
    31561 caatgacatg ggctttctgc tgaacaacaa ttacgcttca aatatccttc atctctatca
    31621 tatggtcatg cgacccaggg gtatcatgat aaatacgatc acccgtttct caaccaccta
    31681 taacaccctt tttgaagcat caaagttaaa gtgcctgact ttcgaagccg ccaacaaaac
    31741 gacttattcc aattactggg taacccattt tgtgctgagc gccagcccgc tggggtttca
    31801 tgattattac gaaccgcggc tctggggatt tatgtatcac aaaccggcct attatagctt
    31861 caatgccatt atcactaccg attcgcggaa gcctttttat gtgaaaagcg tgaccgaatt
    31921 tacaataaca cccggaaacc attcacaggg atgggaagct gaacttatcc cccgcataag
    31981 gttcaacgac aaggttaccc tgacattgga tgttgacttt aacatgctta aaaatgattt
    32041 tggctgggtt aataccctgt acgatactat cggaaaaccg atgatttttt tcgggcgcag
    32101 gacggtagaa acattaaact cggtactgag cgggcagtat acgttcagca ccaaaacagc
    32161 cgttacgcta cggctgcgcc attactggtc gcaggttgat tacgacagtt tctttttgtt
    32221 aaacggcaat ggcgatcttg agagttcaga ttaccagggc gaacatgata ttaatttcaa
    32281 cgcttttacc gccgatgcgc aatttgtttg gtattttgct cccggaagcg aattgagttt
    32341 tgtgtggaag aacgccatta atacttttga tgaaacaatg atccgtaatt actttgataa
    32401 ccttgagaaa acttttagca ctccgcagac caatagcttt tcggtaagga ttttatatta
    32461 tcttgattac cagtatatca gaaagctgat aaaataaaaa agccgcccga aggcagcttt
    32521 tcaataatcc gaaaagggag attaagctac ggtgctcata tgcttgatca tatcgaggat
    32581 tttattgctg taaccccatt cgttatcgta ccaggtaacg agtttaacaa aattaggatt
    32641 cagcatgata cctgcagagg cgtcgaaaat tgaagtacgg ctgtcgccga caaaatcgct
    32701 tgaaaccacg gcgtcttcgg tataccccag gatgcctttc agttcgccat cggcggcggc
    32761 tttaacagca gccttgattt cgtcgtaggt tgcagctttt tccaggcggc aggtgagatc
    32821 aaccaccgat acgtcaaggg taggaacgcg gaacgacatt ccggtaagtt ttcctttcag
    32881 ttcggggata accttggtag cagccttggc agcaccggtg ctcgaaggga tgatgttgcc
    32941 gccggcagca cgcccgccgc gccaatcctt ggccgaaggc ccgtcaaccg tcttttgtgt
    33001 agccgtggtt gcatgaacgg tggtcatcaa accttcaaca attccgaatt tatcattgat
    33061 gacttttacc agaggggcaa ggcagttggt tgtgcagctt gcgtttgaaa taatctgttc
    33121 gccggcatat ttcttatggt taacgccgta aacaaacata ggggtatcat cttttgaagg
    33181 agccgacatg atgacccgtt ttgcccctcc gttgaggtgc gactgacagg cttcagccgt
    33241 caggaaaagg ccagtacatt cggcaacgta atccgcgcca acttccgacc agtttatatc
    33301 ggccgggttt ttacatgcag taacccggat agtccgtccg tttacaacca gatgaccgtc
    33361 tttcacatct acagtgccct tgaattgtcc gtgaacggaa tcatatttca gcatgtatgc
    33421 catgtaattg acatcaataa ggtcgttgat ggcaacaaca tcaatatcat cgcggagcgt
    33481 ggatgcgcgg aagacaaggc ggccgatgcg gccgaatcca ttgataccaa ctttaatctt
    33541 tgtcataatg tattgatttt taggtatgtg aaataaatcc taagtgagat gcaaaggtag
    33601 gcagaaatat tataacagct acagggcctg taaggtttaa ttgggttcct gtaattttct
    33661 aactttatta ctgcagaaag gattgattat aatgggagtt attaggcaag gggcatgggg
    33721 caaggggcaa ggagagcgga gaacggagga cgtgacacga agaacggaga acggagagcg
    33781 taaaacggaa aacgcaaaac ggaaaacgcc aaaggctaac agcccacaga aaacggatag
    33841 tgataaagta acgtcaggag actgaaaatc cttgaacctg ttatctctgc aatggtttca
    33901 gcccgacagc catcctttag aacgctttaa ccttctgact tttttactgc agacaggatt
    33961 gattttaaca ggcaatccgg tatgatgtgt gaaatttgaa ccgtacggca gatttcgtac
    34021 ttttgcacac gatggaaaat atccgaaact tctgtatcat agcccatatc gaccacggta
    34081 aaagtaccct ggccgaccgc ctgctcgaca tgacaggctc aatctccgac cgtgagaaac
    34141 aggaccaggt gctcgacgac atggatttgg aacgcgaacg cggaatcacc attaaaagtc
    34201 atgccatcag gatggactat gcttttgaag ggaagaatta caagctcaac ctgatcgaca
    34261 ctcccggcca cgttgacttt tcctacgagg tttcgcgcgc cattgccgcc tgcgaagggg
    34321 ccttgctggt ggtggatgcc acccagggca ttcaggctca gaccatttca aacctgtacc
    34381 tggccctcga ccatgacctg gagatcattc ctgtaatcaa taaaatcgac agcgacagcg
    34441 ccatgcccga tgaggtggag gaccagattg ttgacctgat cggctgtaaa ccttcagaga
    34501 tcattcgcgc cagcgcccgc accggctatg gcgttgatgc catacttgaa gccattatta
    34561 accgcatccc tcctccaaag ggtgatccgg cagcaccgtt gcaggcgctt attttcgact
    34621 cgttattcaa cgtttaccgc ggcgttatcg cctattttaa agtagtgaac ggcactatcc
    34681 acaccgatga ttacgtgaaa ttcgttgcca cgcagcggga ataccatgcc gacgaaatcg
    34741 gaatcctccg gttacgaccg gaaccttgcc cgctgctgag tgccggggac gtgggctata
    34801 ttatttcggg cattaaaaca gccaaggaaa tcaaggtagg cgacaccatc acccatgccg
    34861 aaactccatg taataatgcc attgaaggtt tcaaaaatgt gaaaccgatg gtgtttgccg
    34921 gcatttatcc taccgacagc gacgattacg aagacctgag ggcctccctg gaaaaactgc
    34981 aactcaacga cgctgccctt tcctttgaac ctgaatcgtc ggcagccctg ggttttggct
    35041 tccgttgcgg gttccttggt ttactgcaca tggaaatcgt gcaggaacgc ctcgaccgcg
    35101 aattcgacat ggacatcatc aacacggtgc ccaatgttat gtacaaagtg cttaccacca
    35161 aaggagacga aattgaggtt cataatcctt ccgggatgcc ggatatcacc actattgacc
    35221 atgttgagga accttatatc ctggcccaga tcatctcaaa gtcggaatat accggcgcca
    35281 tcatgaaact ctgcctcgat aagcgtggaa tgcttaaaaa ccaggtgtac ctgacgcagg
    35341 acagggtgga actgaatttt gaactgccgc tgggcgaaat cgtcttcgac ttctatgata
    35401 aactcaagag catttcaaaa ggatatgctt cgttcgacta tcatatttca ggatacaagg
    35461 aagcatcact ggtcaagctg gacatcctcc tgaacggcga cgccgtagat gcgctctcaa
    35521 cgctcataca cagggataat gcctatgatt ttggcaggcg catctgtgaa aagcttaggg
    35581 aactgatccc ccgtcagcaa ttcgatgtcg ccatccaggc tgctatcggg gccaaaataa
    35641 tagcgcgcga aaccgtgaaa gccgttcgca aagacgttac tgccaaatgt tacggcgggg
    35701 atatcacccg taaacgcaag cttcttgaga agcagaagaa ggggaagaaa cgtatgcgcc
    35761 agatcgggac ggtggaagtg ccgcaaagcg ctttcctggc agtgctgaag cttgattaat
    35821 caaaactgtt cattgacgga ccggtgatca tttcatgccg ggtcggtttc ggatacttcc
    35881 ggatgatgca tcagcatgct taaatcggag gatgaatttg gtataatttc attcaggttg
    35941 taactttata gctgttaaaa ccgtactata tctgaagcct gcttaacaag aagaacacac
    36001 atgacggtag ctgagtacaa ttcgtgtgcc gacctttact ccgacggggt ttatcggttc
    36061 atcctgaaaa atataaagga tgacgatact gccaacgaca tcgtgcagga atcctatgtg
    36121 cggctctggg aacgggtaaa ggatatcagt ttcgagaagg caaaggccta cctgtttaca
    36181 acggcttacc ataccatgat cgatcatatc cgcaggaata aaaggtatag tgaaatggac
    36241 ggaacttacg atacggaatt gcagagcaac aacgcattca gcgacctgaa ggaagtgatt
    36301 gacgaggctg tggcaaaact tcccgaaatt cagcgtaccg tgatactgct tcgggactat
    36361 gaaggttatg cttatgatga aatcggggaa ataaccggat taaacgaatc acaggtcaag
    36421 gtatacatct tcagggcaag aacctttctg aaacagtata tcggaaaact ggatctggtg
    36481 atttaaagca ataagaagcg agaatggaag aaagcaatac cgacagtgtg aagcaaccac
    36541 ctactgacca tagcaaacaa tgaagataaa ccgcgataat tacgaagcat acttcctcga
    36601 ctatcatgaa gggaacctga accctgatga gatggccgcg gtgcttgtat ttatcgggca
    36661 gaaccctgat ctcaaagaag aatttgacga atttgaaaat gtctccctgt ctgttgatga
    36721 ttccgtaagc ttttccgaaa aggattttct gaaaaaaaat gtggaaattt attccggacc
    36781 tgtcacaact gaaaatattg aggaatacct ggttgctgaa acagaggggc ttttaccgga
    36841 tgaaactcta tcgcaactga atgattttgt taaccggaac cctggattcc ggaaggatcg
    36901 caggatttat gcgcatacga ggctggcccc cgccatcgag atcgtatatg gcaataaaca
    36961 cttgttaaaa catgtaattc cgcatgaaaa cccagtgaat gaagcgaatt gcgaagaatt
    37021 catgatccgg aaggtggaag gcttgcttgg cgatgacgaa gccggtgacc tggatgattt
    37081 cctggagcaa aacccccggt tccgtaaaga gatgaaattt ttcggattaa caaagctgca
    37141 gccggataca tcagttactt accgggaaaa ggcctcactg aaacatacca ttgtcccggt
    37201 gcgccgtatt gtttattatg ccatgtcggc cgccgcgtca gtcgcattat tgatgggcgt
    37261 ttactttgcc tgggagagga accggccttc ggataagctt gctgaaaaca agagcgcaaa
    37321 aacggccgtt accccggctg ttattcctga cctgccttcg gatgcccaac atcaggataa
    37381 tgttccggat gcagaaatac ctgcggtaac cacatccgct tcgtccggaa caaccgtgta
    37441 tatagcagca agccaaccgg ttaaagtgca aaacaatgat gacaggacgg ccgttccaat
    37501 gttggaaccc ctggcagaat gcaggatcag ttcaaaaaac tgcattgaac ctgaattctt
    37561 attcatccgt cagagccggt tttatggtag ccggtatatc gatctttata acagtgtaaa
    37621 actttcggaa caaatacaat acgcttcatt gaatgctcct gataaaaatc ctgtccggac
    37681 aatatggaaa ggggtattca accatattga agacaaagtg attgacgaca ggaggacaaa
    37741 gaccgagcct gcgccggaat tatcaatctg gacgctggct gaagccagca taaaagctta
    37801 taataacctg acgcatgatg acctggagct tttgcttcag aaggatgaaa agggcgaagt
    37861 tgtttcctat gccctgattg gtgacaaggt gaatattgaa cgggacattg caaagaagta
    37921 gctgatatga atcagcaaaa aaaaaccggc tgctgccggt ttttttttcg ctgttcctgc
    37981 gtcatcaaag tttgcgtttt atttctatct cttcgtatcc ctcaatgata tcgccgactt
    38041 taatgtcgtt gaaattctct atatttaatc cgcattcata acctgaagcg acttctttgg
    38101 cgtcgtcctt gaaacgtttg agcgacccca gcgcgccggt ataaattacg ataccgtcgc
    38161 ggatcaccct tacgcgggta ttccggttaa tttttccgtc aaggaccata caacctgcca
    38221 cagaacccac ttttgtaata cggaatactt cgcgcacttc cacgttgcat gtaatctttt
    38281 cttcgatttc gggcgcaagc ataccttcga tagcggcttt aatttcgttg atggcagtgt
    38341 atatgatcga atacaggcgg atatcaattt cttcgcgttc ggcaagcttg cgtgcattca
    38401 ggctgggacg tacctggaat ccgataatca cggcgttgga ggctgaagcc agcaatacgt
    38461 cgctttcggt tatggcgccc actgatttat gaatgacatt tacctgaacc tgttcggtag
    38521 ataacttcag cagtgaatcg gacaaggctt ccacggagcc gtctacatcg ccctttacaa
    38581 tgatattcag ctctttaaag tcgccaatgg cgatgcgtcg gccaatttcg tcgagggtaa
    38641 tatgtttttg ggtccgtatg ccttgttcac gctgcaattg gtgccgtttg gtggcgagtt
    38701 gcctggcttc gcgttcgtcg gtcatcacat tgaaattatc gccggcctgg ggggcgccgg
    38761 tcataccaag aagcagcacg ggacatgcag ggcccgcttc tttgatggcc tgattccgtt
    38821 cgttgtacat ggcttttacc ttgccataca ccgatccggc cagcacaacg tcgccaatat
    38881 gaagggttcc gttctgaacg agcattttgg caatataacc gcggccttta tcaagagctg
    38941 attcaattac cgttccgatg gcttttcgtt tgggatttgc tttcagatca agcatttccg
    39001 cttcaagcag caccttttcg agcagcagca ttacgttggt gcctttcttg gctgatattt
    39061 cctggctctg atatttgcca ccccagtctt cgacaaggat gttgagtttt gagagctctt
    39121 cgcggatttt ttcagcatta gcgcccggct tatccatctt gttgatggca aataccatag
    39181 gaacgccggc agcctgggca tgattaatgg cttcgatggt ttgcggcatc accgtatcat
    39241 cggcggcaat tacgataata acaatatccg ttacctgggc tccgcgggcg cgcatagccg
    39301 taaacgcttc atggccggga gtgtcaagga atgtgattcg gcgtccgtcc tcgagtttta
    39361 cctcataagc cccgatgtgc tgcgtgattc ccccggcttc gcctgcaatc acgttggtgt
    39421 cgcggatgta gtcgagcagc gatgtttttc catggtcaac atggcccatg acggtgacaa
    39481 tcggagaacg gggtgacagg tcttcaggcc ggtcttcctc ttcatgactt ccgatagatt
    39541 cctgaacgtc aacgctgaca aattcaacct tgtaaccgaa ttcatcggcc agcaacgcga
    39601 tagtttcagc atcgaggcgc tggttgatgg aaacaaacag ccccaccgac atacaagtgg
    39661 caatgatctg tgtaaccggc acattcatca tatttgccag gtcattggga gtaacaaact
    39721 ccgtaacctt gaggattttc atttcctcgg caatgcgttc ggcttcttcc tccagatgat
    39781 ggctgacagc caaacgcttt tcgcggcggt atttcgaggc ttttgatttg cctgtgccac
    39841 tcaaacgctg caaggtttcg cgaatctgct tctggatttc ctcttccgaa agctcaacct
    39901 ttggttcagg gatgcggccg cgcctttctt ttttcgggcg ctgtttatcg gcttcatgcg
    39961 gctttggctg cgccgttccg gtttcaacgg gcgatccttt gcctttgata cgtttgcgtt
    40021 ttttcttctt gttatctttg ccgtgatcct cactcgacga tgcaacgggt tttcgtccgt
    40081 cggtgcgctg agccggtttg ggcaattcga tcttccctaa tactttgggc ccttcaaggg
    40141 tcggtatatt tgtcttgacg aaattcgggt cttcacggga atagatgccc tgacgagccg
    40201 cgatttcgga tttctggtca gcttttaagg cgtcggcggc gacctctgca accggttctt
    40261 taaccggtat cacagtttct tcaacagggg gctgtatggc ctcttcaaca accttttctt
    40321 caacgggcgg aacttctaca gcctcttccc gggccttttt cttcccgcct ttttctcctt
    40381 ttttcttttt gcttttctga tcaaactggc ccaggtcgat ttttcccagc actttcagtt
    40441 gcggtccgct gggttccccg gtttcaggtt cagtttcagc gacgggctca agcgcttcgg
    40501 gctttggagc aggctgaacc ggaacagccg gttgttccgg ctgtaaaact tcaggttctt
    40561 tttctgcaac cggggccaaa ggaacagcag gagccggagc cggaacgggt ggttgcgcag
    40621 gtggaaccgc cgctacaacg ggagttggtt cgggagttgg ctcaggagtt ggtacctcag
    40681 cgggagccgg agcaggtgca ggaacagcgg gagccggagc aggtgctgga gtaggaacgg
    40741 gagtgggagt gggagcggga gcgggaacgg gaacggaagg agttgccggg acgggcgttg
    40801 gtttaggcgt tggggcaggg gcgggttccg tgataacagg ttcaacggct ttctggggtt
    40861 ccggcttcct gacgggctct gttacctgtt tagcctgtgc ttgcttctgg acatcaaaag
    40921 tcttgatgat cagctcgctg ctttcacggt cttccggttc ttcaggcagc ggggctttcc
    40981 ggtcgtcgat cgaaagggcc tggtggtgca ggtattcaag ttcaattttc tttgaactct
    41041 ccttggtagc cttgtcaggt tggtactcct tcaccagaag gttatacatt tcctgagtca
    41101 gcttggtatt cggactgctg tcgatcttat gccccctttt gtggaggaat tcgacgatag
    41161 tctgaacgcc aacgttaaac tcggttgcgg ctttgctcag ccgtattgtt atgtttcctt
    41221 ctgacatgct gtttaccagg tttctcctat agatcggtat tagtttgcaa aagtacgata
    41281 ttattttcaa tctgaaatta agtaatttgt ttagccggtt taaccattca atgtgcctgt
    41341 tatctagggg tacgggaaag taaatcggca aagcctcttt acttaacgag tttattcaaa
    41401 ctcggctttc aggattttga tcacttcttt gatcgtttct tcctcaagct gggaacgcct
    41461 tacaagctct tcaacatcaa gttcgagtac gcttttggcg gtatcacagc cgatagattt
    41521 cagctcgtcg atgatccact ggtcaatttc atcattgaat tcctggaggt caacatcttc
    41581 gtaatcaaat tcagtatcgc ggtacacgtc aatctcgtat ccggtgagtt tacccgccag
    41641 cttgatgttg tgcccgccct taccgatagc cagcgaaacc tggtcgggtt tcatatacac
    41701 ttcggcgcgt ttgttttctt cgtctatctg gatggaggtg atctttgccg ggctcagcga
    41761 gcgctggatg tacagttgag gattggtggt atagttgatc acatcgatgt tttcattctt
    41821 caactcgcgg acgatcccgt gaatacgcga acctttcatc ccaacgcagg cgcccacagg
    41881 gtcaatccgg tcgtcgtagg attccacggc gatttttgca cgttcgcccg gaacgcggac
    41941 aatgttcttg atggtgataa gcccgtcgta cacttcggga acttccgact cgagcaggcg
    42001 ttcgagaaat accggagaag tgcgggaaag gatgatctgc ggggagtttt tcttcatttc
    42061 aaccctggaa accacggccc ggatcgaatc gcctttgcgg aaaaagtcgc cgggaatctg
    42121 ttcggttttg ggaagcgtga gttccaggta ttcgtcgtca acgatcagga tctcgttttt
    42181 ccatacctgg tacacttcgc cggcaacaat ttcaccgatt ttatccttgt atttcctgta
    42241 aatattgtcc ttttcatact cctgaacctt ggcaaccagg ttctggcgga tggccagtat
    42301 ttcgcggcgg ccgaaatcgt ccatgcggat gggttccgaa acctgctcgc ctacttcgaa
    42361 atcgggctct attttgatgg cgtcggaata ggcaatctgg gtgttttcgt cttcaatttc
    42421 gccatcggca acaattccgc ggttacgcca gatttcaagg tcgcctttat cgacgtttac
    42481 aatgatgtcg atattttcgt cggtgccgta ttttttgatg agcatgtgcc tgaaaacgtc
    42541 ctccaggata cgcatcaatg tttcacggtc gatgttcttg aattccttga actcggagaa
    42601 ggtttccaca aggttgatgt gttccatttc tgccatggct gtatatgttt gaaggttaga
    42661 atttgatgac ttccttggtt tctttgattt cgctgtaagc gaaatggtga gctatttccg
    42721 tgatgatctt tttttctttt ttcttttcga ggatatcgaa tcctttttga tcggcagcta
    42781 ccagcgttcc ggtgatcttt tgctcgtcgg gcagggtgac gatgacttct cggcctgtat
    42841 ttttgatgta ttggcgtaca agcgtatatg ggtgatcgat tcccgacgag gatacattga
    42901 gttcaaaatc ctcggcgtcc cgatcgaggt gcgattcgat ataacggctc agggaagcgc
    42961 aatggtcgat gagcaccgag gtatcgctgt cgataaatac caggatgcgg ttcccgggct
    43021 ttacaatcac atccacgatg aaccggtcgg taccggcgag gtgcgcttcg gctaatttcc
    43081 ttatttgact ttccctgatc atcttccggt gaattaaaca ataaaagagg ggacactcgt
    43141 cccctcctga ttccttcagc cttcttcagc cagggctgaa gaagctttcc ggcagcttcc
    43201 ggagacagtc atacaagtcc caccagaagc ctggtacatt ttagttgtcc gaccaggatt
    43261 tcccgacaat gtcgggattc cggcttcgcc ttcacttctc atcctggtcg gatgggatgc
    43321 gtctcctgtt tttcaacctt gttgtccgac caggctttcc cgacaatgtc gggatttcgg
    43381 cttcgccttc acttctcatc ctggtcggat gggatgcgtc tcctgttttt caaccttgtt
    43441 gtccgaccag gatttcccga caatgtcggg atttcggctt cgccttcact tctcatcctg
    43501 gtcggatggg atgcgtctcc tgtttttcaa ccttgttgtc cgaccaggct ttcccgacaa
    43561 tgtcgggatt ccggcttcgc cttcacttct catcctggtc ggatgggatg cgtctcctgt
    43621 ttttcaacct tgttgtccga ccaggattcg aacctagacc aagagaacca aaatctcttg
    43681 tgctgccatt acaccatcgg acattcggcc tttccgtaaa aaggtgtgca aaagtaatta
    43741 ttttttttta ttgcaactac ccggcgattg aaaaaattaa gagtgacggg cattttacct
    43801 gattcctcta taatcttcta acaattgtgc tacaaggccg gctttggttt ttattccggc
    43861 atcttcatct gaggcaagct gattgatgaa ttccgtgatt tccgtatcaa cctgctttgc
    43921 tatggaggcg ttcaacatga cgcaccacgc cagctttcgc aggaacaact ctttatccgc
    43981 attgtttggg tgtggttggc ttataaactc ctgcagcgat tgcagtactt ccgactgcgt
    44041 ttcctttacc gatttggtta acggatgcac ccagggtact gttataatgt tgacatactg
    44101 cggcttacga gcaggtggca gttgaaagta cggtccagct tgttgaggca tgtagtaatt
    44161 gatcttcccg ttttcgatgg ttacagggca ttgctgcaac atctcacgca aattctgagc
    44221 ttcagacggc atagttatta gca
//
LOCUS       DAOPYV010000019        44009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_718_length_44009_cov_12.492447, whole genome shotgun sequence.
ACCESSION   DAOPYV010000019 DAOPYV010000000
VERSION     DAOPYV010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 44009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44009
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_718_length_44009_cov_12.492447"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1288
                     /locus_tag="PKI35_03915"
     CDS             101..1288
                     /locus_tag="PKI35_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNX43559.1"
                     /translation="MMLKEQIRRLASKYAGEMIEIRRYLHANPELSEKEDNTSAYLRE
                     KLAGWGIPFKANVGGFGIIAEIRGQNPESRTIALRADMDALPVNEINDVAYKSCNEGV
                     MHACGHDVHMSCLLGAARILHEVKEQFEGTLRLIFQPSEEKFPGGALAMIREGVLENP
                     RPVQILGLHVLPTLEAGKAGFRSGRYMASTDEIYLTVKGKGGHAATPDLNIDTVLIAS
                     HIVVALQQVVSRCAPPGIPTVLSFGRFIADGRTNIIPGEVKIDGTIRTFDEAWRSRAH
                     EKITTLAQGIARSMGGECEVFIDKGYPYLVNDEELTSRSKQLAIEYLGAGNVVETEQR
                     MTAEDFAYYSHIIPACFYRLGVKNPEWKEVRNLHTPEFDADEKSIEVGMGLIAWLAVG
                     SGS"
     gene            1381..1770
                     /locus_tag="PKI35_03920"
     CDS             1381..1770
                     /locus_tag="PKI35_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX43560.1"
                     /translation="MKEIKSYTDLDVWIKSRALVKQIYLLTNNFPKEEAYSLTSQIRR
                     AVISIPSNIAEGIARQYNNGTIQFFHISRGSIFELETQIILASDLGYLNDKQLSSVLG
                     EIEDCKKLITGFINYYKNKSRKLNITE"
     gene            1866..2906
                     /gene="mtnA"
                     /locus_tag="PKI35_03925"
     CDS             1866..2906
                     /gene="mtnA"
                     /locus_tag="PKI35_03925"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNX43561.1"
                     /translation="MNINGQPYHTIWLEGSSVFMINQNLLPFEFRIYEARTYRETCRA
                     IITMITRGAGAIGAAAGFAMAQAFMEGEGNSQYTGHARKEIEATRPTARNLFYAAERV
                     FNAGAVSTDAAVEEALRVAQENIDDALKIGEAGNTLIPDGCRILTHCNAGWLGFVDWG
                     SALAPVYKAHREGKKVFVYADETRPRSQGARLTAWELLNEGVPHAVVPDNAGAHLMSK
                     GLVDMVIVGADRIAANGDTANKIGTFEKAILAKEFGIPFYVAAPLSTFDFSISEGSQI
                     PIEERSQDEVLYQAGPDAEGEIHHIRVANPGSSAINPAFDVTPAKYITAFITEKGILK
                     AHPEDLSRLGQS"
     gene            2984..4072
                     /locus_tag="PKI35_03930"
     CDS             2984..4072
                     /locus_tag="PKI35_03930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX43562.1"
                     /translation="MKCCKISLPVFCTAVALCFLSGCSTKSGNNTAAGKKGDQRPASA
                     DAVVVMPGKLDNCIEANGTLLPGEEVEIRSETPGRIVSIDFREGSRVGKGQLLVKIDD
                     RELQAQLKKLLLDEKLAKDDAARKEKLLEINAVSREEYEKAVNQLEVIQANIGLVKTQ
                     ISKTEIRAPFNGVVGLRQVIAGGYVSSATLVSKLQQVDPLKLEFSVPEKYLRDMKVGT
                     PVSYTVEGMNKTFSAKVYAIEPAVDPLTHSITMRALCPNPGNFLMPGAFARIKIVLET
                     LNDAITVPSEAIIPEINGQKVLVCRGGKVTSQKVETAIRNEQMVQIISGLQQGDTVFI
                     SGLLVLKDGMKANPKIVDGSFSGTVVEQ"
     gene            4341..>6028
                     /locus_tag="PKI35_03935"
     CDS             4341..>6028
                     /locus_tag="PKI35_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX43563.1"
                     /translation="MNISSLSINRPVLATVISILIVLMGAIGFLFLGVREYPSVDPPV
                     VSVSTSYTGANADVIESQITEPLEESINGIAGIRSLTSSSSDGRSSITVEFELGVDME
                     AAANDVRDRVSRVTRSLPADIIPPVVSKSDADASPILIYTIQSNTRDLMNLSQVANDV
                     FKERLQTVQGVSEVRIYGEKKYSIKLLLDPAKLAAYRLAPSDVQSAVTRENVDLPAGR
                     IEGYNTELTLRTQGRLTTPEQFNNLIIKEENGTTIRLKEIGEAKLLPENERTIMRGNR
                     GIPMVAVAITPQPGANQIEIADDVYKKMEQIALDLPPDIQTGKMMDTTLTIRKAITEV
                     EETILIAFLLVLMVIFLFLRSWRTTVIPIIAIPISLISGFFIMYIAGFSINILTLLAI
                     VLATGLVVDDAIVVLENIWHKIEGGMNPIEAGHKGSKEIIFAILSTTITLAAVFLPII
                     FMKGVTGRLFREFAVVVAGTVIVSAIVSLTLTPMMSSHLLRKVESHNKLYRVSEKFLN
                     NLTDWYNRMLIRFVAHRRWALVIIVLSLGMILFFGLRIKSELAPMEDKSRLTIQA"
     assembly_gap    6029..6128
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6129..7085
                     /locus_tag="PKI35_03940"
     CDS             <6129..7085
                     /locus_tag="PKI35_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX43564.1"
                     /translation="VEIDRERARALGVSVRDIAETLQLLMSGQRYDYFVMNSKQYQII
                     GQANRENRNKPLDLGSVYVRNNADELIQLDNLIRTKEQSNPPQLYRYNRYVSATVSAQ
                     PEKGVTLGEGIDEMKRIASETLDPSFSTDLSGVSKDYTDSTSNIAFALALALILIFLI
                     LAAQFESYRDPLIIMFTVPLAIAGALLSLTLFGQTLNIFSEIGMIVLIGIVTKNGILI
                     VEFANQRKAAGLPIREAVVNAATRRLRPILMTSIATALGALPIALALGASSRSRVPMG
                     ITIIGGLIFALILTLFVIPALYTYLSRNLKNVEQKPAVANEI"
     gene            7075..8379
                     /locus_tag="PKI35_03945"
     CDS             7075..8379
                     /locus_tag="PKI35_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX43565.1"
                     /translation="MKSKFVLLLVALSLSAGAQEILTLGSALQTGLQNNFSIVLQNNN
                     VQVLKNNNTAGNAGMLPSLGWTAAQNNTVYTTHQESFSGTVKDVRSKMNTSLNSGLQL
                     NWTLFDGMNMFVNRQTLGMMQQIGENQSRIVVENSIVQIARLYYNIIQLKKVIEVDQD
                     AVNLSLERKKIAEAQLALGSGDRQQLLQSTVDLNADSSQLKLQLLLLQNAKSDLNYLL
                     ARDAATPFDIIDSIHLMNLASCEDIVAKAMTQNSELLAAKMDQHLSELDVKSAQSERY
                     PKLNLQSGYNFILQKSQTGFAKFNQSYGPYFGLTASCNIFNGSNTSREIRNARIMLNN
                     SEITVRQASLDIRTSILKLYNSYTTSLELVRLEAGNINAAQQNVDMAFAKYKLGTITD
                     IELREVQKKLLDAQYLMLQAQFDAKNAEIELMQLSGDLIGLF"
     gene            8412..9296
                     /locus_tag="PKI35_03950"
     CDS             8412..9296
                     /locus_tag="PKI35_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNX43566.1"
                     /translation="MKKITLLIFISLIGITAFAQYPRGTVKESLTLDSKILGKKVNYT
                     IYLPFDYETSDRYYPVTYLLHGLSDTDMAWVQFGEANLIADEGAARREIPPMILVMPD
                     GGVSFYINNYDNSVCYEDFFIREFIPYIEKTYRIRAEKRYRGIAGLSMGGFGTLNFAF
                     KYPEMFAACVAFSPALFSEESIMETTREEWESMERPVYGPYKEGEARLTDHYRHNNPF
                     DVTTTKGKNAYNNLRIMIDCGDDDFLANDNCRLHIHLNKLGIKHEFRMRNGAHNWTYW
                     REGLPEGLKFIGQSFHQF"
     gene            9346..9909
                     /locus_tag="PKI35_03955"
     CDS             9346..9909
                     /locus_tag="PKI35_03955"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNX43567.1"
                     /translation="MRTDTCPWPTNDSLMIKYHDTEWGVPLHDDQKLFEFMVLDAFQA
                     GLSWKTILHKRENFRQAFDNFNPEVIAGYSEEKIQQLLRDTGIIRNQAKIRATVGNAK
                     AFLTVKGEFGTFDKYIWQFTGGRTLINELASLSQMPVSSPESDAMSKDLKKRGFKFVG
                     STICYAFMQAAGMVNDHLTVCFRYRAC"
     gene            10183..12051
                     /locus_tag="PKI35_03960"
     CDS             10183..12051
                     /locus_tag="PKI35_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNX43568.1"
                     /translation="MKEFPITAWLPVTRDEMLKRGWEEVDVVIISGDAYVDHPAFGSA
                     LIARIIEAAGYRVAIVAQPNWRDDLRDFKKFGKPRLCFAVSAGNMDSMVNHYTANKRL
                     RSDDAYTPGGVAGFRPDYATTVYTKVLKQLFPDVPVVIGGIEASMRRLAHYDYWSDTL
                     KPSILIDSGADILVYGMAEKGIVEVLQRFEKQKDTAWLTDIPQTAFIVDEAAVDAMAK
                     EMPALVLPSFDQCLRDKAAFARAFREIENESNKKIAKRLIQCSGTKAVVVNPPYPVMT
                     TAEIDAAYDLPYTRLPHPRYKKRGVIPAYEMIRHSVNIHRGCFGGCSFCTLAMHQGKF
                     IASRSEHSIMAEVEKITGMPDFKGYITDLGAPSANMYRMQGFDQDLCAKCSRPSCLFP
                     KICNNLNTDHKPLIQLYQRADSVKGVKKVTIGSGIRYDMLVGRSKEDDRRFSLTAYTQ
                     QLVKHHVSGRLKVAPEHTSDAVLKIMRKPPFKMFHDFKKVFENINRDEGLNQQLIPYF
                     ISSHPGSRLQDMAQLAIETREMGFQLEQVQDFTPTPMTLSSVIYWSGIHPFTGEPVYT
                     PRTKEEKLAQRQFFFWYKPENKDSIRKTLVKLGENKLADSLLGPRNVRIAVKNKKR"
     gene            complement(12172..12630)
                     /locus_tag="PKI35_03965"
     CDS             complement(12172..12630)
                     /locus_tag="PKI35_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNX43569.1"
                     /translation="MKQILVPVDYSDLAAAVIQYTARMAIQFQSRVHIVHIETPVPAF
                     VGNEIGPQVLSQEQRAEETIQQQADLKAMATHLQEKGIETTWELLQGAITDTIIEKAI
                     EIKAELIIVGAHSHGIIYRAFIGSVSSGVLKHAPCPVLVIPEKPSSAFAE"
     gene            complement(12797..14008)
                     /locus_tag="PKI35_03970"
     CDS             complement(12797..14008)
                     /locus_tag="PKI35_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX43570.1"
                     /translation="MKKYYSLIFVLFIVLPAFAQQQHREVGVRPVIVVPTPIERDLEA
                     QFTADVTSGPVPLTVHFTDQSTGGPTSWNWDFGDGASDTLQNPVHAYTQNGVYTVKLT
                     ISNGSQFYALEKKNFITVTINYTGCDTLHCPLPEPLTYYLLTKNNVPLGYVSGNNTFG
                     DKAIADFIDNTSADMLIKGAMIEFSYAKQAALNDENIIIKAWKYDNVHNQPGSLLGSA
                     SVSLSSVISDVAALRPTQVLFDPPLQVDGPFFLGVSLPSQTGDTLVLWTTQSGAITPN
                     TGWVLQSDNKWSAYDSLYTNPTMLVLTDAIYPILCNNGNGSEDHISSRNISITPNPAT
                     DFLRVESKTTSLKNAAYDLIAVTGEKIKTGTFTSWPGAEVLDISSCKPGLYFLRISAS
                     SGILVKRVVIR"
     gene            complement(14073..15407)
                     /locus_tag="PKI35_03975"
     CDS             complement(14073..15407)
                     /locus_tag="PKI35_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43571.1"
                     /translation="MKMKETRNISRNKAYDRLPVHRPDDDLWDQIGHKLDSGEMAAYR
                     DALSKLPVHKVPSGLWDSIEGHLSGRRRRRALLWLGMAAAASLALFTVLRLPEIRPNH
                     SGKAVSPISENIPGVINPADKAVNGNRQARQVQGSSFPAITGHPYIRQHEMLSTSGPA
                     NDGVSCQMPGLATRASLASFAPPSEIKVYYHPGLVAISEKRRMADANRITLSPFPVAD
                     DLPPNRNQTPESTPKQWALALGYLPEALDNGYNPALFHNVDLTASLDMKKYRLSSSLG
                     MAYISERLNYDVTVTMVDNHSANTYGDTANTVCKTISGFEGTESHEFISWDIGGGRKL
                     FTAGKLTTWLNAGAGFAVRLDNTSLRNETVQMLETNYTSDIKSIEMEVPDYNRLNMSL
                     ATGIQFDYRLLKRISITLQPQAKYYFRPIFSNSGTSPDSFSLGFHTGMKFDF"
     gene            complement(15404..15961)
                     /locus_tag="PKI35_03980"
     CDS             complement(15404..15961)
                     /locus_tag="PKI35_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX43572.1"
                     /translation="MQQSAQLNRRKAQSEKDLIQGCSRNERFAQHELYNRYCDAMFTT
                     AYRILNDRDDARDALQEGFIQVFRDIDQFRMESTLGAWIKTIVVRSALRILRKKRSLE
                     LTSLNEVGDEETLRIPETMSGEYLEKAILSLPDGYRAVFLLTEVEGYSHAETARMLGI
                     AEGTSKSQLHHAKNLLRRRISAIIG"
     gene            complement(16030..17271)
                     /locus_tag="PKI35_03985"
     CDS             complement(16030..17271)
                     /locus_tag="PKI35_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNX43573.1"
                     /translation="MKNQSLILALGIFLSLVSCKKEENATSDMKNIPLTTRQQQVVNQ
                     SNAFGFDLFRKVIETSGSENNLMISPLSVSMALGMTRNGAAGTTLDAMNETLGMSGFT
                     EQEINESYQYILGTFSSLDPAVKLSIANSIWYSNTFSVLQPFIETNQTYFGASVTPLD
                     FKDPASVTTINNWVSDKTNQLIPTIIEEIPDGIVMYLIDAVYFKGQWRYKFEKDKTEA
                     KTFYLSDGSEVQAESMLQRANLQYFAGTGFKAVELPYNQGNYNMNILLPEASSSISEI
                     ISQLTQDNWNNWQKNYTEADVQVQLPKFKYDYDEKNMIPVLKALGMEIAIDPNYADFT
                     RMNGLGGLFISEVKHKTYIETNEEGTEAAAVTSVGMSNTSVGNDPQPVYFTADHPFVY
                     IITEKSTGTILFIGTVMNPID"
     gene            complement(17368..18846)
                     /locus_tag="PKI35_03990"
     CDS             complement(17368..18846)
                     /locus_tag="PKI35_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43574.1"
                     /translation="MKIRIIFSSIIAVFIAASVKAQTTEFNLSAYTPPEYVYRSLDFS
                     YYLSGSSNGDVTFRDGVKNGKNAIKYFSTSLQSGYKQYSNSRRYQGSTGLNLYAGFSI
                     NKDDRFGAAYDNKHRQSGNSVKLSYSGQNRFYRNGKAFFEVDPDVGLIHQSIAYSDKN
                     YQNDSLVNDSKTYKWEPSVNIKMPLLAGYGRIEDITDARHAVYILEGLRQTGSLKRGY
                     NDADILSLAAGITKIRNKRFFDSRHQRIYELETLDSLLQSYGLTGDKDAAYFTALNDE
                     WLYSMNTYRYSGIRFSGGIDPQYSLYHYLKEITSNLAAVDDQKRWDESLYRYIGLKAG
                     FKHEKPISLKWQSSLDCSIGYGIEYAMTSFKNLLPDPSGIRKSTQKQQRGNILIGYQA
                     GFYPNSRTWLQAGIDAQVLYYGNGTTKPYATAESDLPHSLIFAGGPDVSLYYYISPRL
                     RLSVNCGFDYYRKWVESYNGEARSVDRSWDWGINSTVTYSLF"
     gene            19006..22356
                     /locus_tag="PKI35_03995"
     CDS             19006..22356
                     /locus_tag="PKI35_03995"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43575.1"
                     /translation="MNHISCLMISVLMAISVTLSGTETVMAQAKAAVKKVAGKTETVK
                     KKATGTKTGAESDTLKSATFSGLKWRSIGPAFTSGRIADFAVNPAKKSEWFVAVASGH
                     VWKTVNNGTTFEPVFDNYGSYSIACVVLDPNNPNTVWVGTGENNHQRSLGYGDGVYKS
                     CDGGATFQNMGLKESRQIGGIVVDPKNSDIVYVAAEGSVWGPGGDRGLYKTTDGGKTW
                     KKILEISENTGVNNIVMDPRDPNVLYASGEQRRRHVFTKIGGGPETAIYKSVNGGETW
                     DKLTNGLPGVDMGGIGLAVSPVNPDIVYAIIEAAGDAGGFFRSVNRGVSWQKMSGHTA
                     QGQYYNEIYCDPKDADKVYSVETVSQVTEDGGKTWRSIGNNKRHVDDHALWIDPDDTQ
                     HFLIGGDGGVYESFDGGKEYLFKSNLPVAQFYRVQVDNSEPFYYVYGGTQDNNSMGGP
                     SRNITASGVVSDEWFVTTGGDGFWSQIDPEDPNIVYSESQYGGMVRYDRKSREAIDIR
                     PEPRKGEDSYKWNWNTPLILSHYSHTRLYCAANKVFQSDDRGNTWQVISDDLTTKIDR
                     NSWPVMGKYWPSDAVAKDISTSLFGTIVSLEESPVKENLLYAGTDDGLIRITGDGGKN
                     WSTASSFPGVPENTYVSDILASKFDENVVFASFDNILRDDFKPYLMKSTDKGKSWVSV
                     SGNLPLNGTVHTIQQDYINPDLLFAGTEFGCFFTVDGGKNWIQLKSGMPTVTVRDIAI
                     QQRENDLVVATFGRGFYILDDYSPLRNFKKEIPGMPGYIFPVKDALMYIQSENKDAQG
                     STYYIAGNPEFGATFTYYIKDVPKTLKEKRKEKESELFKAGKPIPMPSDSLLRAENNE
                     LAPYLTFTVTDESGNVVRVIRKSPSKGLQRINWDLRYQSVRSVHAGDKFDPLSDAWAS
                     IMAMPGKYNVTLSMTASGETTKLAGPVPFTCKTLYTSSLSAGDRKAVTEFYKKVADIT
                     RVMNGTENYAEGLYKRCIEMEQSINSFPEAPDGLLQEVMGLAQQLDIILNTKFNRKSN
                     VPSDEENPPAPVPLNNRLGKITWAAWGNSGAPTQTQQDAYNILMEEFPPVYDQIKKIG
                     QTDIPALEARLVKLGAPVTPGMLPELKK"
     gene            22583..23470
                     /locus_tag="PKI35_04000"
     CDS             22583..23470
                     /locus_tag="PKI35_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNX43576.1"
                     /translation="MIKSMTAYGKGAAEICGKTITVEVRSLNSRQLDINARMPLVFRE
                     KELEIRAEIGRVLERGKIDFSLSVEGEDESAGIIVNRNLAREYYREMTTLAHEFNQQV
                     NADFISTIFRLPDVLKTESRELDQNEWEVVKTALLQALQSADIFRINEGNLLGKDMRM
                     RIELILDYLEQVKPFEENRIVALRQRFEKNRDEFVNTHSNLDKFDPNRFEQEIFWYLE
                     KLDITEEKVRLKKHCDYFIDVLDSAESNGRKLGFVAQEIGREINTLGSKANDAEVQKL
                     VVQMKDELEKIKEQLGNIL"
     gene            23510..24103
                     /gene="gmk"
                     /locus_tag="PKI35_04005"
     CDS             23510..24103
                     /gene="gmk"
                     /locus_tag="PKI35_04005"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNX43577.1"
                     /translation="MTATISRKEKIVIVSAPSGAGKSTVVKHLLGADLGLEFSVSATS
                     RAKREGEQDGREYYFITADEFRQKIENDELLEWQEVYKGSYYGTLKSEVGRIHANGNV
                     PIFDLDVVGGLNVKKMYGEHALALFIQPPSIEVLEQRLRSRNSDSEENLKKRLGKAAW
                     ELEFASKFDRILVNGELETALKNAENAVRQFLGDNDR"
     gene            24144..24731
                     /gene="nadD"
                     /locus_tag="PKI35_04010"
     CDS             24144..24731
                     /gene="nadD"
                     /locus_tag="PKI35_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNX43578.1"
                     /translation="MQELKKTGLFFGSFNPVHLGHMMIANYMYEYSDLEELWFVVSPH
                     NPLKERASLLPDHHRLEMVNLAIGDDERFRSCDIEFKLEKPSYTIDTLTYLQEKYPSR
                     EFILISGTDIFPTFHKWKNWETLLDFYKLYVYPRPGTQDHELTRHGSVKMFDAPMVEI
                     SSSFIRQAIKEGRNVSHFVPEKVWRYLVEMHFYEG"
     gene            complement(24737..25534)
                     /locus_tag="PKI35_04015"
     CDS             complement(24737..25534)
                     /locus_tag="PKI35_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX43579.1"
                     /translation="MNKILQYHKHNISYDISGQGPAIVFLHGFLETKEIWEDFSKYLS
                     GEFTVIAIDLPGHGGSDVLAEIHTMGLMADVIHDILDHHGFREVVIGGHSLGGYIAGE
                     FASKYPSEVKGLFFFHSHAAPDNDETRENRRRSINIVKQNQTGFVNEFIPALFDPKYV
                     ENFKDQITVMQQRAAKMSAEAIIASIAGMRERTGSLPYLFTTETPVLFIIGKQDPRLS
                     YNVVLAQALIPAHSEVLMLDNVGHMGYLEAPRQTMQTIRHFALKCCE"
     gene            complement(25619..27016)
                     /locus_tag="PKI35_04020"
     CDS             complement(25619..27016)
                     /locus_tag="PKI35_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNX43580.1"
                     /translation="MFSPEIYINRRNALRREVKSGVILLPGNAEASMNYPDNTYAFRQ
                     DSNFSYFFGLDHPDLAGIIDIDNGVDYIFGNDVDIDDIIWMGPQPTISERALKAGVKN
                     TAPLSDLGEFMTNVIRRKRPIHFVPCYRGETQLQLSSLLGIKPCRVKDYTSMELIHGI
                     VKLRSVKDDLEIVEMEKAVDIAYLMFVAGMKMAFPGRKEQEITGVMEGISVSHGRPVS
                     FPIILSINGQTLHNHKHENYLETGRMMVMDAGSETSLLYASDITRTVPVGGKFNQRQR
                     DIYEVVLNSHLKSISVTKPGTFYYDCHMKAAETMASGLKNLGLMRGNVQDAVAAGAHA
                     LFFPHGLGHMLGMDVHDMEGLGQIYVGYDSEIQPSSQFGTSSLRMGRRLQPGFVVTNE
                     PGIYFIPELIDQWKAANKFTEFINYDKVDEYRDFGGIRIEDDILVTKDGCRILGKPIP
                     KTVAEVEETMKEGLS"
     gene            27152..27484
                     /locus_tag="PKI35_04025"
     CDS             27152..27484
                     /locus_tag="PKI35_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43581.1"
                     /translation="MKKIIGIILMVLAFGITSQAQDTQAKSAQDWNKQGLEHFKKFEY
                     KTAYECFSKALELKSDYGEAYYNRGMTWFQLPAGSYTSHDGCSDLKKAKELGFKLKKD
                     DLSKNGCE"
     gene            complement(27561..28853)
                     /locus_tag="PKI35_04030"
     CDS             complement(27561..28853)
                     /locus_tag="PKI35_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNX43582.1"
                     /translation="MRYKPIPSEMYINNRARLTAKMKPGSIAIVNANDEMPRTGDQTF
                     GYRQNSDLFYLTGLDQEKCILVLCPGHPLESMREILFTVKTSDLMVTWNGYKYTFDEV
                     RKLSGIKTVKWIDDFDFILKELMSHVQTVYLNQNEYIKFFTDVPYRDLRFAEKMRREF
                     PVHTYERLAPLITSMRTAKQPGELEQIKKACDITGDAFRRVLRFVKPGMMEYQVEAEM
                     IHEFISRGCNGHSFSPIIASGKNSCILHYVNNDLPCKDGDVLLLDFGAEYGLYGADMT
                     RTIPVNGKFSPRQRQVYEAVLRVLKAGMTMLTPGNTNDNWHKAMCSLIEKELVGLGLI
                     TEDDIKNQDPDNPAFFRYYMHGSGHFLGLDVHDVGTKQQPFEYGAVMTCEPGIYIPEE
                     GIGIRLENDVIVADKPINLMENIPVEPDEIEAIMLSGK"
     gene            29006..29671
                     /locus_tag="PKI35_04035"
     CDS             29006..29671
                     /locus_tag="PKI35_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNX43583.1"
                     /translation="MSKKVLIYSSITKKVIMAFAGLFLITFLALHLSINLFLLKSDGG
                     AAYMTAVRFMITNPVIKIMEIFLFGGFFIHILIGITIQIYNWLARPRRYRVEGFSHTS
                     FFSKYMIHTGAIILTFLVIHFTNFYFKKLGWVSPPEGVGREDFYQMAMMLFSNSFYAI
                     LYTVLMIFLGFHLNHAFQSAFQTLGLNHSKYTPFIKGLGTVYSIAVPLGFACIPLYIL
                     ILK"
     gene            29723..31663
                     /locus_tag="PKI35_04040"
     CDS             29723..31663
                     /locus_tag="PKI35_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNX43584.1"
                     /translation="MAILDSKTPPGPLDKKWSNYKDNINLVNPANKRKLDIIIVGTGL
                     AGASAAASLGALGFRVKAFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTI
                     KGGDYRSREANVYRLAEVSGTIIDQSVANGVPFAREYGGLLDNRSFGGALVSRTFYAR
                     GQTGQQLLLGAYSAMSRQVKLGTVELFPREEMLDLVIVDGRARGIITRNLITGEIHRH
                     AAHAVVLATGGYGNVFYLSTNAMGSNTSAIWKAYKRGACFANPCMTQIHPTCIPMHGD
                     YQSKLTLMSESLRNDGRIWVPKNKEDAKAVRQGKLKPNDIPEANRDYYLERRYPAYGN
                     LVPRDVASRAAKERCDAGFGVNETGLAVFLDFADSIKRLGRSTIEERYGNLFQMYQKI
                     VNEDPYSTPMMIYPAVHYTMGGLWVDYELQSTITGLFVAGEANFSDHGANRLGASALM
                     QGLADGYFILPYTLQNYLADQIKVPLFSTDQPEFEETEKQVRSRLEKLMNIKGQHSVD
                     YFHKKLGRIMWENAGMGRNRGNLQKAISLIRELRAEFWKDLKIPGEQNAMNTELEKAS
                     RVADFLELGELLALDALKRDESCGGHFREEFQTPEGEALRNDSDFMYVSAWEYNGDDN
                     EPILNKEPLFYESIEVKQRNYK"
     gene            31736..32512
                     /locus_tag="PKI35_04045"
     CDS             31736..32512
                     /locus_tag="PKI35_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNX43585.1"
                     /translation="MNFTLKIWRQKNARSKGRFVTYKIQEISPNCSFLEMLDQLNERL
                     VAGNEEPVAFDHDCREGICGACSLYVNGHPHGPDSAITTCQLHMRKFRDGDTIIVEPW
                     RARPFPVIKDLMVDRSAFDAIIQAGGFISVNTGGVPDANAILISKEKADYSMDAAACI
                     GCGACVAACKNASAMLFVAAKVSHLALLPQGRVEAAGRVQSMVKKMDELGFGNCTNTG
                     ACEAECPKQVSITNIARMNREFLTAKVCSQKKRVMAGGGF"
     gene            32614..33837
                     /locus_tag="PKI35_04050"
     CDS             32614..33837
                     /locus_tag="PKI35_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNX43586.1"
                     /translation="MKFSEIIGQEPVKARLLQSVKENRVSHAQLFLGPEGSGKLAMAI
                     AYAQYINCRQRTETDSCGTCPSCVKYSKLIHPDLHFLYPINKTKEVDKEPLSIDFLPQ
                     WREFLSENFSYVSLQDWYEKIGIEKKQGIINADDANYVIKALNLKSYEAEYKIVIIWM
                     AEKLFHAAAPKLLKSIEEPADKTVFILVSENPDSIPQTILSRTQLVKLNRTGDDAVAE
                     FLEDKLNLDASEASSIALVADGNAKEALDMARLSSEQQTTGKGDSANPKSDPDRERFG
                     LFREWMRRCWVITDSRIMLKDYNLLQETVASIVSDGSREKQKDFLAYGLKMLRSCLHY
                     NMGNRHLVKHAPYEMEFIANFSRYIHIRNIQSFDEEFNQAIFHIERNAAGNLIFTDLS
                     HIVARLLKIAPPPQR"
     gene            33969..35087
                     /gene="ricT"
                     /locus_tag="PKI35_04055"
     CDS             33969..35087
                     /gene="ricT"
                     /locus_tag="PKI35_04055"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNX43587.1"
                     /translation="MDEKKVNDIFSNTILARGCCLAGKTDDENNPVFSHGCAKLDVQN
                     WLKGLPFPPDFKEFDCIEVRFKNNRKDFFRLQAGIEVHDGDIVAVEASPGHDIGVVTL
                     TGELVRMQMRRKNVNPAKEDIRKVYRRARQADIEKWMGAVAKEDNAIFKCRRIAADLG
                     LKMKVNDVEYQGDDTKAIFYYTADDRVDFRELIKVLAEEFRIRIEMRQIGVRQEASRL
                     GGIGTCGRELCCSTWLTGFHSVATAAARVQQLSHNPQKLAGQCGKLKCCLNYEYEAYL
                     DAIKNFPDTSIPLKTNQGEAIVMKIDVFAGIIWYAYKTNESEIFAIPVDQVEKIQSLN
                     KRGKQVEKLEDFARKQEKKSELDLIVGQDELTRFDKHK"
     gene            35105..35620
                     /locus_tag="PKI35_04060"
     CDS             35105..35620
                     /locus_tag="PKI35_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HNX43588.1"
                     /translation="MPLFKSLTSPVKSFLQLALTFIPLIVLAIGFHSCHNNQVYRETR
                     HISSDGWKPADSISFTFQVDDTLTPLNFYFNVRNTTRYSYQNLFFFITTKYPGGAFSR
                     DTAECILAGIDGRWLGKGKGKVRDSKFLFRKEVRFHKTGEYTLIVNQAMREELLKGVS
                     DIGLVIEKPEE"
     gene            35665..37998
                     /locus_tag="PKI35_04065"
     CDS             35665..37998
                     /locus_tag="PKI35_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNX43589.1"
                     /translation="MKKKKNQSASFEDWIRRFWIAFAASLVLMAMFFTALSLGWLGFM
                     PTFEELEAPESYLATEIISADQKQLGTFYIENRLNVHYSDLSPWVVKALIATEDARFE
                     KHSGIDGKALLRVMYGMITGTDQGGGSTITQQLAKNLFPRKYHRTIIGTGFSKLREWI
                     TAIKLERHYSKEEIAALYLNTVDFGSQAYGIKSAAITYFGKEPSELNPQESALLIGIL
                     KAPSYFHPVRNKQRAIHRREVVLHQMMKYHYLTRAEYDSIKVLPLDMSHYKLRDHNSG
                     SATYMREYLRIMLSASKPHRDDYDDVADYVADSVRWEKDPAYGWINKNHKPDGGTYNL
                     YKDGLRIYTTIDSRMQQYAEAAVTKHLSTDLQPAFFKHWKGNPHAPFDLNDKEEIKKL
                     LAASRKRSDRYRNMKRSGYPADSIDLAFRTPIPMRVFSWKGEIDTIMSPDDSIKYHKW
                     FLQAGLMSVEPQTGYVRAYVGGINFRYFKYDHVMIGRRQVGSTFKPFVYTVAMQNGES
                     PCTRVANVQYTIDSGDGKIWAPKNSGKYKEGQMVTLKEALANSINWISAYLIKRYSPQ
                     SVVQIARKMGIVSPLPAVPSICLGTPDITLAEMVGAMNTYANDGIYIHPILITRIEDK
                     HGNIISQFAPYQEEAMSEETAYLMLILMKGVVESGTGARLRGTYGFTNPIAGKTGTTQ
                     SNSDGWFMGLTPDLVTGVWVGGDDRTIRFRNTSLGQGANMALPIWAYYMKAVYNDSTL
                     QLSKGDFEKPEAPLSVEINCAAAAQAGEGDVNDYDKY"
     gene            38081..39562
                     /locus_tag="PKI35_04070"
     CDS             38081..39562
                     /locus_tag="PKI35_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43590.1"
                     /translation="MDQRTEKIIANILTLAFLAFFCMLAVTSTAIFEGADNIAHYNLS
                     HYSFRHPELLLDHWGKPLFTILSSPFSQAGFVGTKLFNVLTGVAAAYFAYRYLRSQKV
                     QMAFAVIPFVLFVPMYFMMMQSALTEVLCSFILVVSAFCFADKRYILSAILLSFVPVS
                     RTDAVIILPVYAAALLWFRQYKALPFLLTGTFVFSISGWFQYGDILWLIHNNPYKGAS
                     SLYGKGDLLHFVEQIPYICGIPLTILTVTGIFSILFRFFRNNAHPGEPATADVFVLLL
                     PALALLAAHSYLWWKGLGGSAGLTRVLTPVLPLTAIMAYKGFDEITWRIPVKWVRWLI
                     LLVVLFFIILVPVRKYRPPAKLQKADLLMSQTADWIKSEGLQNRNIFYYDPEVAFFND
                     RDPWNHEKGRQIIRDGFVNETIFGEGSLIIWDAHFGPHEGRVPLSLLLENKQLRLIKI
                     LLPNPPFTIYGNEEYCIYVFLKDRTHAPHDNFAIEKELRSTHF"
     gene            39631..41253
                     /locus_tag="PKI35_04075"
     CDS             39631..41253
                     /locus_tag="PKI35_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43591.1"
                     /translation="MMPKRKSRLSLIACWLIIFLVVGANLKYRHWENRYKIIYVDVLH
                     YYAYLPATFIHHDLSLEFIRTNNSYYSQYFWPRVLPDKKLIIMTTMGMSYMYAPFFLA
                     VHYPLKWLGEPALGYSPPYKMALIASSIFWFALGLFFLRKLLLKYFSDFTTSLTLIIT
                     ALATNLFYYVTDEPAMSHAYGFALFAMFIYFTDRWFEKPKFGYSLALGVLAGVIALIR
                     PSNAVIVLILLLWGVDSFQSLGKRLGLFLRQYRMALVMVAAAILVWVPQVLYWKYASG
                     HYFVFTYGEKGKFFFNNPQFFRVLLGFRKGWLIYTPVMIFSIIGLIPLWKKHRNLFWP
                     VVIFAAVNLWIVSSWWLWWYGGSYGMRALIESYAVMALPLAAFIAWVTENRSILVKAA
                     VAVLALVFVTHNFFQITQYHTGAIDAIKMTREAYFASFLKRNPNTRQQRLLVYPDYKA
                     AEYGRYPRPSSVRDSLYSGLLDREHAVRKIEQEIRSGEESMKMMQQKALARNVSIDSA
                     ISIDANYLYDLKVKKGEIPANPGKFKIFDWWY"
     gene            41263..42174
                     /locus_tag="PKI35_04080"
     CDS             41263..42174
                     /locus_tag="PKI35_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX43592.1"
                     /translation="MIRIDENDATINTVGMTQTYTYSVIIPAYNEETAIGDLLNRMTE
                     LGITEKYEVIVIDDGSTDRTCEIVSRFPVRLIRHETNKGYGASLKTGIRHASGQKVMT
                     LDSDGQHDPAYLSEMVKLLAEYDMAIGERTADSFQVKNRRGGKKIIRKVGEYLVEQKL
                     PDFNSGFRGFDRQLILSMLHLMPNGFSFSTTSTLAFLKEGYSIGKFPIKVNGRQGRAS
                     SVKFIKDGTKTMLLLFRIIMLFNPLKVFFPASAFITMVGAIWGIHGYIVFDRFSNTAI
                     IVIILGMFLFFFGLLADQIALLNRKKA"
     gene            42171..42902
                     /locus_tag="PKI35_04085"
     CDS             42171..42902
                     /locus_tag="PKI35_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX43593.1"
                     /translation="MTDFSKYQNCWSDRDVEAHWDRVAGRYVEENDKVKQTHDQRFSF
                     SLPYLDLYCGARVLNITSRDGEAAAYISAKEPSVEVTNAEISQKLMDVALKLNPSIRQ
                     VKISTYSHLPFSDEQFDRILTLETLEHAANPMEFLSELHRISIPDARMVLSCPPATSE
                     IPYRFYTAVFGGHGEGPHRFPPSKQVKEWLKLSGWKLTFHKGTVLMPVGPGWFKSLGE
                     KLIDKTQGTFISELGIRQFYVCEKY"
     gene            42889..>44009
                     /locus_tag="PKI35_04090"
     CDS             42889..>44009
                     /locus_tag="PKI35_04090"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNX43594.1"
                     /translation="MKSIENLQHVIRHNLCNRCGTCVGLSGGRIVFSDKTGKYLPEIT
                     AELPRELADRIWEACPGQKFDFPEYRKLFYPDAPHFHLYTGPYRDLFIGYATDELTRR
                     NSASGGILTAVLLWLLDKKWIDGAVVTAMSEKEPWLAQSFIATTAEEILRAAQSKYII
                     TSVNEILPQIEAFQGKLAYVGLPGQVQSIRKLQATHDVSVQNIRYVFGPFYGNTLHFS
                     SIKSFLRSYGEKDFHDISRLWFRYGEWPGNMRIEMKSGRVIQLPKFHANYLIPFHILK
                     NSLLCTDLSNEFTDISGGDAWAPVYEERGKGFSMVFSRSGQGKEILEQMQREGVISLQ
                     PIDENEAITMHSHGYDLKKRGSFIRMKFRKLAGLPNPDYG"
BASE COUNT        11587 a        10277 c        10878 g        11167 t
ORIGIN      
        1 actgcaaaag tggggacaaa aagcataccg ttgaaattca ggattaataa aatagtcttt
       61 ggccgtttca ttcttacttt tgcagcaacc aataccatgt atgatgttga aggaacaaat
      121 caggcggctg gcatcgaagt atgccggtga gatgatcgaa atccgccgtt acctgcatgc
      181 aaacccggaa ctatcggaaa aagaagacaa tacttcagcc tatctcaggg aaaaacttgc
      241 cggttggggc atcccgttta aggccaatgt cggaggattt ggcatcatcg cagaaatcag
      301 gggacaaaac ccggaaagcc gtaccattgc cttgcgcgcc gatatggatg ccctgcccgt
      361 taacgaaatt aatgatgtcg cctataaatc ctgtaatgaa ggtgtgatgc atgcctgcgg
      421 gcacgatgtg cacatgtcat gtttgttggg cgctgcccgg atactgcatg aggtaaagga
      481 acagtttgaa ggtaccttgc ggctgatttt tcaaccatcg gaagaaaaat tccccggcgg
      541 ggcgctggcc atgatcaggg agggcgtcct cgaaaatcct cgtcccgtcc agattctcgg
      601 cctgcatgta ttgcccacgc tcgaagcggg aaaagcagga ttcagatcag gacgctatat
      661 ggcttcgacc gacgaaattt accttacggt aaagggtaaa ggaggccatg ccgccacacc
      721 tgacctgaat atagatacag tgctgattgc atcccacatc gtggtggccc tccagcaggt
      781 cgtcagccgt tgcgcgcctc ccggtattcc gacagtgctg tcgttcggaa ggttcatcgc
      841 cgacggccgc acgaatatca ttcccggtga ggtgaaaatt gacggtacta tacggacctt
      901 cgacgaagcc tggcgttcga gggcgcacga aaagataacc accttggcgc agggcatagc
      961 ccgaagcatg ggcggggaat gcgaggtgtt tatcgacaag ggttaccctt atcttgtaaa
     1021 cgatgaagaa ctgacgtccc gctcaaaaca gcttgccatt gaatatcttg gggccgggaa
     1081 tgttgttgaa accgagcaac gcatgaccgc cgaggatttt gcatattatt cccatatcat
     1141 cccggcatgc ttttaccggc ttggcgtaaa gaacccggaa tggaaggaag tgaggaacct
     1201 gcatactccc gaattcgatg ccgacgagaa aagcattgaa gtgggcatgg gcctgatagc
     1261 atggctggcg gtgggaagtg gttcgtgatt cgtgattcgt aatttgtgat tcgtaattag
     1321 taattagtga ttcgtggaat gttgtaataa aaaatttaaa taaaactaaa tcagttattt
     1381 atgaaagaaa ttaaatctta taccgatttg gatgtctgga ttaagagccg tgctttagta
     1441 aaacagattt atttgctgac caataatttc ccaaaggaag aagcctattc tttaacatca
     1501 cagatcaggc gtgccgttat ttcgattcct tcaaatattg cagaaggtat tgccagacag
     1561 tataacaacg ggacaattca attctttcat atatcaagag gctcaatctt tgaattagaa
     1621 acccagataa ttctggcatc tgatttagga tatctcaatg ataaacagtt gtcatcggtg
     1681 ttgggagaaa tagaggactg taagaaattg atcaccggat tcataaacta ctataaaaac
     1741 aaatcccgga aattaaatat aactgaatag gatcatatct ctaataattt aatgacaacg
     1801 atgccttgca catcaggagc aacgttacgt taacaacaac gaatcactat catcaaccac
     1861 caacaatgaa tattaacggc caaccttatc atactatctg gctcgaaggc tcttcagtct
     1921 ttatgattaa ccagaacctt cttccttttg aattcaggat ttatgaggcc aggacatatc
     1981 gcgaaacctg ccgggccatt atcacgatga tcacccgcgg agcaggcgcc atcggagctg
     2041 ctgcaggctt tgccatggcc caggcgttca tggaaggtga agggaacagt caatataccg
     2101 ggcatgcccg caaggaaatc gaggcgacgc gtcccactgc acgcaacctt ttttatgctg
     2161 cggaaagagt tttcaatgcc ggcgccgtgt cgacagatgc ggcagtagag gaagctcttc
     2221 gcgtggcaca ggaaaatatt gacgatgcgc tgaaaattgg tgaagccggg aacaccctga
     2281 tccctgatgg ctgcaggata ctcacgcact gcaatgccgg atggctggga ttcgtggatt
     2341 gggggagcgc cctggcgcct gtttataaag cgcatcgcga aggcaaaaag gtatttgtct
     2401 atgccgatga aacccgtccc cggagtcagg gcgcaaggct cacagcctgg gaactcctga
     2461 atgaaggcgt tccccatgcc gtcgtccctg ataatgccgg agcgcatctt atgtcgaagg
     2521 gattggttga catggtcatc gtaggggctg accggattgc cgccaacgga gatactgcga
     2581 acaagatcgg cactttcgaa aaagcaattc ttgcgaagga attcggtatt ccgttttatg
     2641 ttgcagcgcc tttgtcgacc tttgattttt ccatttctga aggcagtcag atacccatcg
     2701 aagaaagaag ccaggatgaa gtgttgtacc aggcaggccc ggatgctgaa ggtgaaattc
     2761 atcatatccg tgtggcaaac ccgggttctt cagccatcaa tccggcattc gacgtcacac
     2821 ctgcaaagta tattacggca tttatcaccg aaaagggaat tttaaaggct catccggagg
     2881 atttatcccg tctgggacaa agttaacttc atgttaatcc ttgccggatg cgtttttact
     2941 gaaaacataa tccgtaattt tgaaggcata tattccctcc tctatgaaat gctgtaaaat
     3001 ttctctacct gttttctgta ccgcggtagc actgtgtttt ctttccggtt gctcgacaaa
     3061 atcgggcaat aatacggctg ccgggaaaaa aggcgatcag agaccggcat cggccgatgc
     3121 tgttgtcgtg atgccgggca aacttgataa ctgtattgaa gccaatggca ccttgctccc
     3181 gggagaggaa gtggaaatac gcagcgaaac acccggacgg attgtcagca ttgattttcg
     3241 cgaagggagc cgtgtcggca aaggtcaact tttggtaaaa attgacgacc gtgaattgca
     3301 agcgcaacta aagaaattgc tgcttgatga aaaactggca aaagatgatg ctgcacggaa
     3361 agaaaagttg ctcgagataa atgctgtgag ccgcgaagaa tacgaaaagg cagtaaacca
     3421 gttggaggta attcaggcca acatcgggct tgttaagacg caaatatcga aaacagagat
     3481 cagggcccct tttaacggtg tggtgggatt aaggcaggtg atcgccggcg gttacgtttc
     3541 ctcggctacc ctggtatcaa agctgcaaca ggttgatccg ctcaaactgg agttctcggt
     3601 gccggaaaaa taccttcggg atatgaaggt cggaacccct gtcagttata cggttgaggg
     3661 catgaataaa actttttcgg caaaagtgta tgccattgag cctgctgtgg atccgcttac
     3721 acattccata acgatgcgcg cactttgccc gaatccggga aattttctca tgcctggcgc
     3781 ctttgcgcgt ataaaaatcg tgctcgaaac actgaatgac gccattacgg tgccttccga
     3841 agccattatt cctgaaatta acggacaaaa agtgctggtt tgccgcggcg gtaaggttac
     3901 ttcgcaaaaa gtggaaacag ccatccggaa cgagcaaatg gtccagatta tctccggatt
     3961 gcagcagggt gatacggttt ttatcagcgg attgcttgtg ctgaaagatg gaatgaaggc
     4021 caatccgaaa attgttgatg gttctttctc tggaaccgtc gtagagcaat aacgaaatta
     4081 agcagccaca atcatatcaa aacaagatat gcaggacaaa acacgaaaaa tcatcggttt
     4141 ctgcttcggg atagccggcc tggtgctttt aatctggaat gcatatgttt acctcagcca
     4201 ccaggaaaca agcaaggtgt ctgtaatgac aggtttattg ttttgtattg agggtgccgt
     4261 actgatcaag aaaagcaaaa gtaatgccgg tcaataaata gccgttttac attcatgaat
     4321 atcaactgcc cttccaccag atgaatatat cctccctcag catcaaccgc cccgtcctgg
     4381 ctaccgtaat ctccattctt attgtgctca tgggggctat cggattcctg tttctcggcg
     4441 ttcgcgaata tcccagcgta gacccgcctg tggtttcggt aagcacttcc tataccggcg
     4501 ccaatgccga tgttatcgaa tcgcagatca ccgaaccctt ggaagaatcc ataaatggca
     4561 tcgccggcat ccgttctctt acttcatcca gcagcgacgg acgcagcagc attacagtgg
     4621 aatttgaact tggcgtggat atggaagcag ccgccaacga cgtccgcgac cgtgtttcgc
     4681 gcgtgacgcg aagcctgccg gccgacatca ttccgccggt ggtatccaag tcggatgccg
     4741 atgccagccc catcctgatt tataccattc aaagcaatac ccgcgacctg atgaacctct
     4801 cgcaggtggc gaacgatgtg ttcaaggaac ggctgcagac tgtccagggc gtcagtgagg
     4861 tccggatcta tggtgagaaa aaatattcca tcaaactgct tctcgatccg gcaaaactgg
     4921 ctgcataccg gctggcgcct tccgatgtcc agagcgccgt tacacgcgaa aacgtggact
     4981 tgccagccgg ccgcatcgaa ggatataata ccgaattgac ccttcgcacc cagggcaggc
     5041 ttaccacgcc ggaacagttc aacaacctga ttattaaaga agagaacggt accactatcc
     5101 ggctgaagga aataggggag gcaaagctcc ttcccgaaaa tgagcggacc atcatgcgcg
     5161 gcaacagggg catcccgatg gttgcagtag ccatcacacc acaaccggga gccaaccaga
     5221 tagaaattgc cgacgatgtc tataaaaaaa tggagcagat cgctctcgac cttcctccgg
     5281 atattcaaac cggcaaaatg atggatacca cgctgaccat ccgcaaggcc atcaccgagg
     5341 tggaggaaac catactgatc gctttcctgc tggtgctgat ggtaatattc cttttcctgc
     5401 gaagctggcg tacaacggtt atccccatta ttgccatccc gatttcgctg atcagcgggt
     5461 tttttatcat gtatatcgcc ggtttttcca tcaacatcct cacgttgctg gccattgtac
     5521 ttgccacagg actggtggtt gatgatgcca ttgttgtact ggaaaacatc tggcacaaga
     5581 tagaaggggg catgaatccg attgaagccg ggcacaaagg ttccaaagag attatttttg
     5641 ccattctgtc cacaacgatc accctggcgg ccgtgttcct gccgattatt tttatgaaag
     5701 gggttaccgg caggctgttc cgcgaatttg ccgtcgtggt ggccggtacg gttatcgttt
     5761 cagctattgt ttcgctcacg cttacaccta tgatgagctc gcacctccta cgaaaagtgg
     5821 aaagtcacaa caaactttac cgggttagcg agaagttcct gaacaacctg actgactggt
     5881 acaacaggat gcttatccgc tttgttgccc acaggcgctg ggcgctggtg atcatcgtac
     5941 tatctctggg gatgatcctg tttttcgggt tgcggatcaa atcggaactg gcgccgatgg
     6001 aggataagag ccggctcaca atacaggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnngt ggaaattgac cgtgaacgtg cccgcgccct gggcgtcagc gtgcgcgata
     6181 ttgccgaaac cctgcaattg ctgatgagcg gacagcgcta cgactatttc gtcatgaatt
     6241 cgaaacagta ccagatcata ggacaggcaa accgtgaaaa ccgtaacaag ccgctggatc
     6301 ttggttcggt atatgtgagg aacaatgctg atgaactgat ccagctcgat aaccttatca
     6361 ggacgaaaga gcaaagtaat ccgccacagc tataccgtta caaccggtat gtttcggcca
     6421 ctgtttcggc ccagccggaa aaaggggtga ccttgggcga aggcatcgac gaaatgaaac
     6481 ggattgcatc cgaaacgctg gatccgtctt tttcaaccga cctgtcaggc gtttcaaaag
     6541 attataccga tagtaccagc aatattgcct ttgccctggc tttggcgctg atcctcatct
     6601 ttctcatcct ggcagcgcag tttgaaagtt accgcgatcc gctcatcatc atgttcacgg
     6661 taccgctggc aattgccggg gcgttgctgt cacttaccct gttcgggcaa acactgaata
     6721 ttttcagcga aataggtatg atcgtgctta tcggcatcgt caccaaaaac ggtatcctga
     6781 tcgttgagtt tgctaaccag cgtaaagctg ccggactgcc gatccgtgaa gccgtagtca
     6841 atgctgcaac ccgccgtttg agaccgatcc tgatgacaag tattgccacg gcgcttggcg
     6901 cactccccat cgcactggcg cttggcgctt cctcgcgaag ccgtgtcccc atgggaatta
     6961 ctatcatcgg cggactgatc tttgcactga tactcaccct gttcgtcatt ccggccttgt
     7021 atacttacct gtcccgaaac ctgaaaaatg tcgaacaaaa acctgccgtt gccaatgaaa
     7081 tctaaattcg tactcctgct tgttgccctt tccttatctg ccggtgcaca ggaaattctt
     7141 acccttggat cggccctgca aacgggactg cagaataatt tttcaatcgt actgcagaac
     7201 aataacgttc aggtgcttaa aaataacaac accgcaggca atgcgggcat gctgccttcc
     7261 ctgggctgga cggccgcgca gaacaatacg gtttatacca cgcaccagga atcattcagc
     7321 ggcaccgtaa aagatgtcag gagcaaaatg aatacatcgt tgaattcagg gcttcagctc
     7381 aactggaccc ttttcgacgg gatgaacatg ttcgtaaacc ggcaaaccct cggtatgatg
     7441 caacaaattg gggaaaacca gtcgaggatt gtcgttgaga actccatcgt tcagattgcc
     7501 agactctatt ataatattat ccagttgaag aaggtaattg aagtggacca ggatgctgta
     7561 aacctttcgc tggagcgaaa aaagatcgct gaggctcaac tggcgctggg ctcaggagac
     7621 aggcagcaac tgcttcagtc aacggtggac ctgaatgccg acagcagcca gctcaaactg
     7681 cagttgttgt tactgcagaa cgccaagagt gatctgaact acctgctagc ccgggatgct
     7741 gctacccctt ttgatattat agattccatc catttgatga acctggcttc ctgcgaggat
     7801 atcgtagcca aagcaatgac ccagaattcg gaactcctgg cggcaaaaat ggatcagcac
     7861 ctttcggaac ttgatgtgaa aagcgcacaa tcggaaagat atccgaaact gaacctgcag
     7921 tcgggttata attttatcct ccagaaatcc caaaccgggt tcgcgaaatt caaccaaagt
     7981 tacgggcctt atttcgggct tacggcatcc tgtaacattt tcaacggttc aaatacgagc
     8041 cgcgaaatcc gcaatgccag gatcatgctc aacaacagcg agatcactgt ccgacaggcc
     8101 tctctcgata tcaggaccag catcctgaaa ctctacaatt cttatactac ctcgctggaa
     8161 ctggtcaggc ttgaagccgg gaatatcaat gctgcacaac aaaacgtgga tatggctttt
     8221 gcgaagtaca aactgggaac tattaccgac atagaattgc gtgaagtgca gaagaaactg
     8281 ctggatgcgc agtacctgat gttgcaggcc cagtttgatg caaaaaatgc tgaaatcgaa
     8341 ctgatgcaac tcagcggcga cctgatcgga ttgttttaat tccaacaaac ggtttcttac
     8401 taaaatccct gatgaaaaaa attacattgc tgattttcat ttcactaatc ggcattactg
     8461 cctttgccca gtaccccagg ggaaccgtga aagaaagcct caccctggac agtaaaatcc
     8521 tgggtaaaaa agtaaactat accatttatc tgccgtttga ttatgaaacc tcggaccgtt
     8581 attacccggt gacctatttg ctgcatggat tgtccgacac cgatatggcc tgggttcagt
     8641 tcggcgaggc caacctgatt gccgatgaag gcgccgcaag acgcgaaatt ccgcctatga
     8701 tcctggtaat gcccgatggc ggtgtttcgt tctatatcaa caattacgac aactcggtgt
     8761 gttacgagga tttctttatc agggagttta ttccgtatat cgaaaagaca taccgcatca
     8821 gggccgagaa gcgctaccgg ggtattgccg gcctgtccat gggcggtttc gggacgctga
     8881 attttgcctt taaatatcct gaaatgtttg ctgcctgtgt tgctttcagc ccggcgctgt
     8941 tctcggaaga gagtataatg gaaaccaccc gtgaggaatg ggagtctatg gaaaggccgg
     9001 tttacgggcc atataaggaa ggggaagccc gccttacgga tcattacagg cataacaatc
     9061 ctttcgatgt aacgacaaca aaagggaaga atgcatacaa caacctgcgt atcatgatcg
     9121 attgcggcga cgatgatttc ctcgccaatg acaattgcag gctgcatatt cacctgaata
     9181 aactcgggat aaaacacgag tttcgtatgc gaaacggcgc ccataactgg acttactggc
     9241 gggagggact accggagggc ttaaaattca tcggacaaag tttccaccaa ttctgatgac
     9301 ctgtatcatt gtattcctga aataatttcg aaaaagtaac aatctatgcg aaccgatacc
     9361 tgtccctggc ctaccaacga ttcccttatg atcaaatacc acgatacgga atggggcgtt
     9421 ccattgcatg acgatcaaaa actgttcgaa tttatggtgc tcgatgcctt tcaggccggg
     9481 ttgagctgga agaccatcct ccataaacgg gaaaatttca ggcaagcttt cgataatttt
     9541 aatccggaag tgattgccgg gtattccgaa gagaagattc agcaattgct cagggatacc
     9601 ggaattatcc gcaaccaggc gaaaatacgg gcaaccgtcg gaaatgcaaa ggcattcctg
     9661 acggtgaagg gggaattcgg cactttcgac aaatacattt ggcagtttac gggaggaaga
     9721 accctgatca acgagctggc ttcgctttcg caaatgccgg tttcatcgcc tgaatcggat
     9781 gccatgagca aagacctgaa aaagcgggga ttcaaatttg tgggttctac catttgttat
     9841 gctttcatgc aggctgccgg aatggtaaac gaccatctca ccgtatgttt caggtatcgt
     9901 gcctgttaat gtcttgcgga cagattcttg taaatttctg taaacaggaa gatcttcctt
     9961 ttccgaaagg ccggatttaa gatgcataaa tatcccagcc cggttctccg gacgtcctgt
    10021 cggcctggcg ctaaatttga attattccgg ttcacttcac ctgacaatcc atccgatatc
    10081 agttcattaa caaaatcagg cattgaatac gttgttccgg ctgcactctc atagccgtct
    10141 tttggcctat ctttgcagtc atctgatatt aaaaccgccg tcttgaaaga atttcccatc
    10201 acagcctggc ttccggttac ccgcgatgaa atgttgaaac gcggctggga agaggttgac
    10261 gtagtcatca tttcaggcga cgcttacgtg gatcacccgg cgtttggcag tgccctgatc
    10321 gccagaataa tcgaagctgc cgggtacagg gttgccatag ttgcgcagcc aaactggcgc
    10381 gacgatctca gggatttcaa aaaattcggg aaacccaggc tgtgttttgc cgtatcggcc
    10441 ggcaacatgg attcgatggt aaaccattac accgccaata aacggctgcg cagcgacgat
    10501 gcctatactc ccggcggcgt ggccgggttt cgtcccgatt atgccacaac ggtctataca
    10561 aaagtcctca aacaactttt tccggatgtt cctgtcgtga ttggcgggat agaggcctcc
    10621 atgcgccggc tggctcacta cgactactgg agcgatacgc tcaaaccgtc aatcctcatc
    10681 gatagcgggg ccgatatact ggtttacgga atggcagaaa aaggaattgt ggaggtgctg
    10741 caacgtttcg aaaaacagaa ggatacagcc tggctgacgg atattccaca gacggctttt
    10801 atagtggatg aagctgctgt tgatgcgatg gcaaaggaaa tgcctgcact tgtgttgccg
    10861 tcgttcgatc aatgcctcag ggataaagca gcctttgccc gggcatttcg tgaaatagag
    10921 aacgaatcga ataagaagat tgcaaagcgg cttatccaat gttcaggaac aaaggcagta
    10981 gtggtaaatc cgccttatcc tgtcatgacc accgccgaaa ttgatgcagc ctacgacctg
    11041 ccttatacaa ggttgccgca tcccaggtac aaaaagcgcg gggtgattcc ggcctatgag
    11101 atgatccgtc actcggtgaa cattcaccgg gggtgtttcg gaggttgttc cttttgcacc
    11161 cttgccatgc atcaggggaa atttatcgcc agccgttccg aacattctat catggcggaa
    11221 gtggaaaaaa tcaccggtat gcctgacttt aaaggatata ttaccgacct gggagcgccg
    11281 tcggccaata tgtaccgcat gcagggcttc gaccaggacc tctgtgccaa atgcagccgt
    11341 ccgtcatgtc tctttccgaa gatctgcaat aacctgaaca ccgatcataa gccgctgata
    11401 cagttgtatc agagagctga ttctgtaaaa ggggttaaga aagtaaccat cggcagcggg
    11461 atcagatatg atatgctggt tggcagatcg aaagaggatg acaggcgctt cagcctgaca
    11521 gcctatacac agcaattggt gaagcatcat gtttcgggcc ggctgaaagt ggcgccggaa
    11581 cacaccagcg atgccgtgct gaaaatcatg agaaagcctc cgttcaagat gttccatgat
    11641 tttaaaaagg tttttgagaa tattaaccgg gatgaaggat taaatcagca gctcatccct
    11701 tattttattt cgagccatcc gggaagccgg ctgcaggaca tggcccaact ggccattgaa
    11761 accagggaaa tgggatttca gttggagcag gtccaggatt tcacgcctac tcccatgacg
    11821 ctgtcgagcg ttatatattg gtcaggaata cacccgttta caggcgaacc ggtctatacg
    11881 ccccgtacta aggaggaaaa actggcccag cgacagttct ttttctggta taaaccggaa
    11941 aataaggact caatcaggaa aacccttgtt aaacttggcg agaacaaact ggccgactcc
    12001 ctgctcgggc caagaaatgt aagaatcgct gttaagaata agaaacgctg acatctttct
    12061 ggcaaaagcc tcaacattcc ccggcatgat tgagaaatgt catgagtccc cgaaactgcc
    12121 aaccggacta agaacgctgg cgcctgattt cccgacgatg aacgatgata cctattcagc
    12181 gaaagcggat gaaggctttt ccgggattac cagaaccggg caaggcgcat gtttcagcac
    12241 gccactgctg acgcttccga taaatgccct gtaaatgatg ccgtggctgt gggctcccac
    12301 aatgatcagt tcggctttaa tttcaatggc tttttcgatg atagtatctg ttatggcgcc
    12361 ctggagtaac tcccaggttg tttcgatgcc tttttcctga aggtgcgtag ccatagcttt
    12421 caggtcggct tgttgttgta tggtttcttc agccctttgt tcctgagaaa gcacctgagg
    12481 gccaatctca tttccaacaa aagcaggaac aggcgtttca atatgaacaa tatgcacgcg
    12541 gctttgaaat tgtatcgcca tcctggctgt atactgaatc accgcagcgg cgagatcgga
    12601 ataatcaacc ggtaccagaa tctgtttcat ggctcaaatc agattaatga tacaaatata
    12661 atgaataaat atggaaaagg caagcaaata tttgatatac aggttgaaat gtgaacggat
    12721 taaatatcaa taagttaaac aagtttattt gatggcgttg ttatcagtat cgaaatctat
    12781 taccggatta gtgcaattac ctgataacca ctcgtttcac caatattccg gaggaggcgg
    12841 aaatgcgcag gaaatataat cccggtttac aggagctgat atcaaggact tcggcgcccg
    12901 gccaggaagt aaaggtgccg gttttgattt tttcaccggt tacagcgata aggtcatacg
    12961 cggcattttt tagagaagtc gttttggatt caaccctcag gaaatcagta gccggattag
    13021 gcgttatgct gatgtttctg gatgaaatat ggtcttccga tccgttgccg ttgttacaca
    13081 ggatcgggta gattgcatcg gtaagcacca gcatagttgg atttgtatac aggctgtcat
    13141 acgcggacca tttgttgtcc gactgaagca cccatcctgt attgggtgtt attgccccgg
    13201 actgggttgt ccataaaacc aaagtatcgc ctgtttgaga aggcagggaa acgcccagga
    13261 aaaacgggcc gtcaacctgc aaaggggggt caaacagcac ctgtgtcggc ctcagggctg
    13321 caacatcaga aataaccgag gacaaggaaa cggaagcaga tccgagaaga cttcccggtt
    13381 ggttatgaac gttgtcatat ttccatgctt tgataataat gttctcatca tttagcgcgg
    13441 cctgtttagc atagctgaat tcgatcattg cccccttgat cagcatatct gcagaggtat
    13501 tatcgatgaa gtcagctatg gctttatccc cgaaagtgtt atttccggag acatatccca
    13561 gaggaacatt attttttgtc agcaggtaat aggtaagcgg ctcgggaagc gggcagtgta
    13621 atgtgtcaca accggtataa tttatagtaa cggtgataaa attctttttt tcaagcgcat
    13681 aaaactggct tccgttcgaa atggtcagtt tcacagtata aactccgttt tgggtgtaag
    13741 catggaccgg gttttgcagc gtatcggaag cgccgtctcc aaaatcccag ttccagcttg
    13801 tagggcctcc ggtcgactga tcggtaaaat gcaccgtaag cggcacagga cctgacgtga
    13861 catcagcagt gaactgcgct tcaaggtccc gttcgatggg ggttggaaca acaatgaccg
    13921 gcctgacgcc gacttcacgg tgttgttgct gagcaaaggc aggaagaacg atgaataata
    13981 caaatatcag ggagtaatat tttttcataa caagctgttt attggtaata acagggaaag
    14041 ccggttaaag actgagggcg gtttttgcag gcttaaaaat caaatttcat gccagtatgg
    14101 aatcccaggc tgaaggaatc gggggaggta ccggaattgc tgaaaatcgg gcggaaataa
    14161 tactttgcct gcggctgaag ggtgatgcta atgcgcttca ataacctgta atcaaattga
    14221 atgccggtgg ccaggctcat gttcagcctg ttgtagtccg gaacttccat ttcaattgat
    14281 tttatatcag aggtgtaatt tgtctcaagc atttgaaccg tttcattcct gaggcttgtg
    14341 ttgtcgagcc ttacagcaaa cccggcgcct gcattcagcc aggtggttaa tttcccggcg
    14401 gtaaaaagtt tacgtccccc gccaatgtcc catgatataa attcatggct ttcggttcct
    14461 tcaaatccgg agatagtttt acatacggta ttggcagtat cgccataagt attcgcagaa
    14521 tggttatcaa ccatcgttac ggtaacatca taatttaacc gttccgagat ataggccatt
    14581 cccagactgc tgctgagcct atacttcttc atgtcgaggc ttgccgttaa atccacgttg
    14641 tgaaacaaag caggattata accgttatca agggcttcgg gcagatagcc cagtgcaagg
    14701 gcccattgtt tcggagtgct ttcaggcgtc tggttccggt ttggaggcag atcatcagca
    14761 accggaaaag gagacagtgt gatacggttg gcatctgcca tccgtctttt ttcggaaata
    14821 gccaccaatc cgggatgata ataaacttta atttcggagg gcggggcaaa tgaggccaga
    14881 gaggctcttg ttgccaagcc cggcatctga catgaaacgc cgtcatttgc cggtccggaa
    14941 gttgacagca tttcatgttg ccggatatat ggatggcctg tgattgccgg gaaagaagat
    15001 ccctgaacct gccgggcctg ccggtttcca ttaacagctt tatcagcagg attgattact
    15061 ccgggtatat tttccgaaat aggggaaaca gcttttcctg aatggtttgg ccggatttcg
    15121 ggcaatctga gaacggtaaa gagcgccagc gaagctgccg ccgccatgcc gagccataac
    15181 aaggcgcgac ggcgcctgcg gccggaaagg tgtccttcaa tactatccca taatcctgaa
    15241 ggaactttat ggacaggcaa cttcgatagt gcatcccggt aagcagccat ttctcctgaa
    15301 tcgagcttat gcccgatctg gtcccacaga tcatcatcag gacggtgaac cggcaacctg
    15361 tcgtatgctt tgtttcgtga tatgttcctg gtttctttca ttttcatccg atgatggccg
    15421 aaattcttct tctcagcagg ttctttgcat gatgaagctg cgatttggag gttccttccg
    15481 ctatgccaag catccgggcg gtttccgcgt ggctgtagcc ttcaacttcg gtaagcagaa
    15541 aaacggccct gtatccatcc ggcaacgaca gaattgcctt ttccaggtat tccccgctca
    15601 tcgtttcggg tatgcgcagg gtttcctcat cgccgacttc attgagggaa gtaagttcca
    15661 gggatctttt ttttcgcagt atccgcaaag ctgaccttac aacgattgtt ttaatccatg
    15721 ccccgagggt tgattccata cggaactgat caatgtcgcg gaatacctgt atgaatccct
    15781 cctgcagggc gtcgcgggcg tcgtcgcggt cgttcagtat cctgtatgca gtggtaaaca
    15841 tggcatcgca atatcggttg tacagttcgt gctgggcaaa gcgttcattc cgtgaacagc
    15901 cctgtatcag gtccttttca ctttgcgctt tccgccggtt aagctgtgct gactgctgca
    15961 agaatggtag tattagggcg ctttttatcc tcagttgttt aacatccttc agatcaccgt
    16021 aaagttacat cagtctatcg ggttcattac cgtaccgatg aaaagtattg ttccggttga
    16081 tttctcggta atgatataga caaaaggatg gtcagcagta aaatataccg gttgcggatc
    16141 gtttccgaca gatgtgtttg acatgccgac agaagttact gctgcagcct ctgtgccttc
    16201 ttcattggtt tcgatgtagg ttttgtgttt tacctccgag atgaacaatc cgccgagacc
    16261 gttcattcgt gtgaaatcgg catagttggg atcaatagct atctccattc ccagcgcttt
    16321 taaaacaggg atcatgttct tttcgtcgta gtcatactta aatttcggca actgtacctg
    16381 aacatcggct tcggtatagt ttttctgcca gttgttccag ttatcctgtg taagctgtga
    16441 aatgatctcg ctgatcgaag aggaggcctc aggcagcaga atattcatgt tgtagttgcc
    16501 ctggttgtaa ggcagttcca cggctttaaa accggtaccg gcaaaatatt gaaggttggc
    16561 ccgttgcagc atgctttctg cctgaacctc actgccgtcg gagaggtaaa atgttttggc
    16621 ttcggtttta tccttttcga acttgtagcg ccattgccct ttgaagtaca cggcatcaat
    16681 caggtacatc actatgccat ccggtatttc ttcaatgatg gtcgggatca gttggttggt
    16741 tttatccgaa acccagttgt tgatggtggt gaccgaagcc ggatctttaa agtcaagcgg
    16801 ggtgaccgat gccccgaaat aggtctggtt ggtttcgatg aatggctgca gtacgctgaa
    16861 agtattgctg taccagatgg aattggcaat tgaaagcttc accgccgggt cgagcgacga
    16921 aaaggtccca aggatatact ggtaggattc attgatttcc tgttcggtaa agccgctcat
    16981 gccgagcgtt tcattcatgg catcgagggt tgtccccgct gcgccgttgc gcgtcatgcc
    17041 aagcgccatg ctgacgctca ggggtgaaat catcaggttg ttttcgctgc ccgaagtctc
    17101 gatcactttc cggaaaaggt caaatccaaa ggcattgctt tggttcacta cctgttgttg
    17161 cctggtagtg aggggaatgt ttttcatatc ggaggttgca ttttcctctt ttttacatga
    17221 aaccaggctg aggaatatgc ccaaggcaag aatgagagat tgatttttca tgagtatggt
    17281 attttatact aaagggaatc accgaaccct tttcgttgca taaccccgaa taaaaattta
    17341 aaaaacaata tccgaatgaa tcccggtcta gaacaggcta taggtaacag tggaattgat
    17401 cccccaatcc catgagcgat caacggaccg ggcttcgcca ttataacttt caacccattt
    17461 cctgtagtaa tcaaaaccgc agtttacaga aagccttagc ctggggctta tataatagta
    17521 aaggcttacg tcaggccctc cggcaaaaat cagggaatga ggtaaatctg attctgctgt
    17581 tgcatacggt ttggttgtcc cgttgccgta atacagaacc tgtgcatcaa tacctgcctg
    17641 gagccatgtc ctgctgttgg gataaaagcc tgcctggtaa ccgatcagga tatttccccg
    17701 ctgctgtttt tgggttgatt tcctgatgcc ggaaggatca ggaagaagat ttttaaatga
    17761 agtcattgca tattcaatcc cgtaaccgat tgaacaatcg agcgaagact gccatttcag
    17821 ggaaatcggt ttttcatgtt taaaacctgc cttcaggcca atataacggt ataacgattc
    17881 atcccatctc ttttgatcat caacagcagc caggtttgaa gtaatctctt tcaggtaatg
    17941 ataaagactg tattgaggat caattccgcc ggaaaacctg atgcccgaat acctgtatgt
    18001 attcatggag tatagccatt catcattcag ggcagtaaaa taagcggcat ccttatcacc
    18061 ggttaaaccg tatgattgca gcagtgaatc cagcgtttcc agctcgtaaa tccgttgatg
    18121 ccgcgaatca aaaaaccttt tattcctgat tttcgttatc ccggcagcca ggctaaggat
    18181 gtcggcatcg ttatacccgc gtttcaggct tcccgtttgt cttaatcctt ccaggatata
    18241 tacggcgtga cgggcatcgg tgatgtcctc aatacgtccg taaccagcca gcaggggcat
    18301 ttttatattt accgatggtt cccacttgta tgttttactg tcgtttacca gtgaatcatt
    18361 ctgataattc ttatcggagt atgcgatgga ttggtgaatt aaaccaacat ccggatcaac
    18421 ctcgaaaaag gccttaccgt tcctgtaaaa ccggttttgg ccgctgtagc tcagcttcac
    18481 ggaattgccg ctttgccggt gcttattatc ataagccgcg ccgaaccggt catctttatt
    18541 gatgctgaaa ccggcgtaga gattcaggcc ggtgcttccc tgatatctcc ggctgttcga
    18601 atactgttta taaccggact gaagacttgt gctgaaatat tttatggcgt ttttcccgtt
    18661 tttgactcca tcccggaaag tcacatcccc gttcgaactg cctgaaaggt aatagctgaa
    18721 atccagcgag cggtaaacat attcgggagg ggtataagcg gagagattaa actctgtagt
    18781 ctgggctttg accgatgcgg caatgaaaac agcgatgatc gaactgaaga taatccggat
    18841 tttcatgaga tgtttctttg aatatgtatg catagttagg aaatattttc ggaaacctgc
    18901 tttgctgaac ctgaattttg catatttgtc atataatccg ggagtattct ttcctgccgg
    18961 aaaaaatacc atctaagcct tttataactt ttaaaacaaa ccaggatgaa tcatatttca
    19021 tgtttaatga tcagtgtgct gatggcgata tcagtcactt tatcaggcac ggaaacggta
    19081 atggcccagg ctaaagccgc agtaaagaag gtagcgggga aaaccgaaac tgttaaaaag
    19141 aaagcgactg gtacaaaaac cggtgcagaa tccgataccc ttaaatcggc gactttcagt
    19201 ggtttgaaat ggcgttccat aggtccggcc tttaccagcg ggcgtattgc cgattttgcc
    19261 gtgaacccgg cgaaaaaaag cgaatggttc gttgcagtag catcaggcca tgtatggaaa
    19321 acagtgaaca acggtacaac tttcgaaccg gtatttgata actacggttc ctattccatc
    19381 gcatgcgtgg tgctggatcc caacaatccg aataccgtct gggtcggaac cggagaaaat
    19441 aatcatcagc gttcgctagg ctatggcgac ggcgtgtata aaagctgtga cggcggcgcc
    19501 acttttcaga acatggggct gaaggaatcg cgccagatag gcggtattgt ggttgatccg
    19561 aagaattccg acatcgttta cgtggctgcc gaaggttccg tatgggggcc tggtggcgac
    19621 cgtggcctgt acaaaactac cgacggcggt aaaacatgga aaaaaatcct tgaaatcagc
    19681 gagaatacgg gcgtcaacaa cattgtgatg gatccccgcg acccgaatgt cctgtacgct
    19741 tcgggtgagc aacggcgcag gcatgttttc accaagattg gcggagggcc cgaaacggcc
    19801 atctataaat cggtcaatgg cggagaaacc tgggataaac ttaccaacgg gcttccgggc
    19861 gttgatatgg gcggtatcgg actggccgtt tctccggtca atccggatat cgtctatgct
    19921 atcatagaag ctgccggcga tgcaggaggc tttttccgtt cggtaaaccg gggcgtttcc
    19981 tggcaaaaaa tgagcggaca tacggctcag ggacagtatt ataacgaaat ttactgcgat
    20041 ccgaaagatg ccgataaagt gtattctgtt gaaacggtct cgcaggtaac agaagatggc
    20101 ggtaagacct ggcgttcgat cgggaataat aagcgtcatg tcgacgacca tgccttatgg
    20161 atcgatccgg atgatacaca gcattttctg attggcggcg atggcggtgt ttacgaatcc
    20221 tttgacggcg gtaaagagta tctcttcaag tcgaacctgc cggttgccca gttttacaga
    20281 gtgcaggtcg ataattccga gccgttttat tatgtgtatg gaggcaccca ggataataac
    20341 agcatgggcg ggccgtcgcg caacattacc gccagcgggg tggttagtga tgaatggttt
    20401 gtgacgaccg gcggtgacgg gttctggtcg cagattgacc cagaagaccc gaatattgtt
    20461 tattccgaat cgcagtacgg cggcatggtc cgctatgacc ggaaaagcag ggaagcgatt
    20521 gatatcaggc ctgaaccgcg aaagggcgaa gattcctata aatggaactg gaatacgccg
    20581 cttatcctta gccattattc acatacaagg ctgtattgtg ctgccaataa ggtatttcaa
    20641 agtgacgatc gcgggaatac atggcaggta atcagcgacg acctcacaac aaaaattgac
    20701 cggaacagtt ggcccgtgat gggaaaatac tggccatccg atgccgtagc caaagatatc
    20761 agcacatcgc tgtttggaac cattgtatcg ctcgaagaat cgccggtaaa ggaaaacctc
    20821 ctgtacgccg gaacggatga tggtttgatc aggataacgg gtgacggtgg aaaaaactgg
    20881 tcaacggcgt cgtctttccc gggagtgccc gaaaatacgt atgtaagcga tatcctggca
    20941 tcaaaatttg atgagaatgt cgtgttcgct tcattcgata atatcctgcg cgacgatttt
    21001 aagccttacc tgatgaagag taccgataaa ggaaaaagct gggtttctgt ttcgggcaac
    21061 ctgccgctga acggaaccgt tcacaccatc cagcaggact atatcaatcc cgacctttta
    21121 tttgcaggca cagaattcgg ttgcttcttt accgttgacg gcggaaagaa ctggattcaa
    21181 ctgaaatcgg gcatgccgac ggttaccgtt cgtgatatag caatccagca gcgtgaaaac
    21241 gacctggttg tcgccacgtt tggccgcgga ttttacatcc tcgacgatta ttcaccgttg
    21301 cgaaacttca aaaaggaaat tcccggtatg ccgggttata ttttcccggt taaggacgcc
    21361 ctgatgtata tacaatcgga aaacaaggat gcccaggggt caacctatta cattgccggg
    21421 aatccggaat tcggcgccac ttttacctat tatattaaag atgttcccaa gacgctgaaa
    21481 gagaaaagga aagaaaagga atcggaattg tttaaagcag ggaagcccat acctatgcct
    21541 tccgattcgc tgttacgcgc cgagaacaat gagttggcgc cataccttac ttttaccgtt
    21601 accgacgaat cgggcaatgt ggtgcgtgtg atcaggaaaa gcccgtcgaa aggcttgcag
    21661 cggatcaact gggatttaag gtaccagtcg gtgcgcagtg ttcatgccgg cgacaaattc
    21721 gatccgcttt ctgatgcctg ggccagtatt atggcaatgc ccggcaaata caatgtaacc
    21781 ctcagcatga cagcctccgg tgaaactacg aaacttgccg gcccggttcc gtttacctgc
    21841 aaaacattgt atactagttc attatcagcc ggtgatcgta aagctgtcac ggaattttac
    21901 aaaaaggtgg ccgatattac acgcgtcatg aatggcacag aaaactacgc cgaagggtta
    21961 tataaacgtt gtattgaaat ggagcagtcg atcaacagct tcccggaagc tccggacggc
    22021 ctgttgcagg aagtgatggg acttgcccag caactggata ttatcctgaa cacaaaattc
    22081 aaccgtaaat caaatgtgcc gagtgacgag gaaaatccgc cggcgcctgt gccgttaaac
    22141 aaccgtctgg ggaaaataac atgggcagcc tggggaaatt cgggcgcgcc tacccaaacc
    22201 cagcaggatg catataacat cctgatggaa gaattcccgc cggtatatga tcagataaag
    22261 aaaataggcc agacagatat tccggcgctg gaagcccggc tggtgaaact gggggcgccc
    22321 gtgacgcctg gaatgctgcc cgaactgaaa aagtaacccc gttttgccaa taacccgatt
    22381 gagaaactat atctagaatc atgccggatt ttgatgtata gaaaccctgc gattgagaat
    22441 ggtctttttg cgtgattgat tccagacggg gaaagcaggc gaccgaatcc gccggaacta
    22501 ttgatacagt ttctttttta taaccggctg ttcaccggga aggcctttcc ttcctatctt
    22561 tgcttcgaac aaatacccgg atatgatcaa atcaatgact gcctacggga aaggtgcggc
    22621 agaaatttgc ggaaagacaa taacggttga agtccgttcg ctgaacagcc gtcaactcga
    22681 tattaacgcc cggatgcccc tggttttccg tgaaaaggaa ctcgaaatcc gtgctgagat
    22741 cggtcgggtg cttgaacgcg gtaaaattga cttcagcctt agcgtggaag gcgaagatga
    22801 atctgcaggc atcatcgtta accggaacct ggcccgtgaa tactaccgtg agatgacaac
    22861 gctggcccat gaattcaacc agcaggtaaa tgccgatttc atctctacca ttttccggtt
    22921 gcctgatgtt ctgaagaccg aatccaggga acttgaccag aacgagtggg aagttgtgaa
    22981 aactgcgctg ttacaggcgc ttcagtcggc ggatattttc cgcatcaacg aagggaattt
    23041 gctcgggaaa gatatgcgga tgcggattga actcatcctt gattacctgg aacaggtcaa
    23101 gcctttcgag gaaaaccgga ttgttgcctt acgccagcgt tttgaaaaga acagggacga
    23161 attcgtgaac acccattcaa acctcgataa atttgatccg aaccgatttg aacaggaaat
    23221 tttctggtac ctggaaaagc tggatatcac cgaagaaaag gtgcggctga aaaaacactg
    23281 tgattatttt atcgatgttc ttgacagcgc cgagagtaac gggcgtaaac tgggattcgt
    23341 tgcccaggag atcggccgcg aaatcaacac ccttggttcg aaagccaacg atgctgaggt
    23401 acagaaactg gtcgttcaaa tgaaggatga acttgaaaag atcaaagaac aattgggaaa
    23461 catactttag gttagtttcg cattgaaaat ttttaatcca gcctgaaata tgacagcaac
    23521 aatttcccgg aaagaaaaga ttgttatcgt ttcagcgccg tcaggggccg gaaagagcac
    23581 tgtagtgaag caccttctcg gggcagatct tgggcttgaa ttctcggttt cggctaccag
    23641 cagggcaaaa cgtgaagggg aacaggacgg ccgggaatac tattttatta ctgccgatga
    23701 attcaggcaa aagatcgaaa acgatgagct gcttgagtgg caggaagtgt ataaaggcag
    23761 ttattacggt acgctgaagt cggaggtggg tcgcattcat gctaacggca atgtccccat
    23821 ttttgacctg gatgtagtcg gagggctgaa tgtgaagaaa atgtacggtg agcatgcgct
    23881 tgcccttttc attcaaccgc catccatcga agtgctcgaa cagcgactgc ggtcgcgtaa
    23941 cagcgacagc gaagaaaacc tgaaaaagcg gctcggcaaa gcagcctggg aattggaatt
    24001 tgcatcaaaa ttcgacagga tactggtcaa cggagaactc gaaactgccc tgaaaaatgc
    24061 cgaaaatgcc gtacggcaat tcctcgggga taatgaccgg taattcggtt tgccggtaaa
    24121 cagagcaacc acaataagcc tgtatgcagg aactgaagaa aacaggattg ttttttggct
    24181 cattcaaccc ggtgcacctt gggcacatga tgattgccaa ttacatgtat gagtattcgg
    24241 accttgaaga actatggttc gtcgtctcgc ctcataatcc gctgaaagaa agggcgtcac
    24301 tgcttcccga ccatcacagg ctggagatgg taaatctcgc catcggcgac gatgaaagat
    24361 ttcgttcctg cgatatcgaa ttcaaacttg aaaagccatc ctataccatc gataccctta
    24421 cctatttgca ggaaaaatac ccgtcgcgcg aattcattct tatttccggc accgatatct
    24481 tccctacctt ccataaatgg aaaaactggg aaacgcttct cgatttttat aagctttatg
    24541 tatatccgcg tccggggact caggatcatg aactgacgcg gcatggttcg gtgaaaatgt
    24601 tcgatgcccc gatggtggaa atatcgtcat cctttatcag gcaggccatc aaagaaggac
    24661 gaaatgtttc gcacttcgtt cctgaaaaag tatggaggta cctggttgag atgcacttct
    24721 atgaaggctg aaagccttat tcgcagcatt taagggcgaa atgccggatc gtttgcattg
    24781 tctggcgcgg ggcttccaga taccccatat gtccgacatt atccagcatc agcacttccg
    24841 agtgagccgg tatgagggct tgcgccagga cgacattata cgaaaggcgg gggtcctgtt
    24901 tcccgataat aaataaaacc ggtgtttcgg ttgtgaacag gtaaggcagg ctgcctgtcc
    24961 gttcgcgcat ccctgcaatg gaagcgatga tggcttcagc cgacattttg gcggcgcgct
    25021 gctgcatcac agttatctga tccttaaaat tttcaacata cttcgggtca aaaagggcag
    25081 ggataaattc attcacaaac ccggtttggt tctgtttcac gatgttgata ctgcggcggc
    25141 ggttttccct ggtctcatca ttgtcgggag ctgcatgcga atggaagaaa aacaggcctt
    25201 ttacttctga tgggtatttt gaagcaaatt caccggcgat ataaccgccc aggctatgcc
    25261 cgccgattac tacttccctg aaaccgtgat gatcaagaat atcatggatc acatctgcca
    25321 tgaggcccat ggtgtggatt tcggccagca catcgctgcc gccgtgaccg gggaggtcga
    25381 tggctatcac cgtgaattcc cctgacaggt attttgagaa atcttcccag atttcctttg
    25441 tttcaaggaa tccgtgcagg aatacgatag ccggtccttg cccggaaata tcatagctta
    25501 tattatgctt atggtattgc agtatcttgt tcatgtctct taatgataaa ttgccgggaa
    25561 aggaggcatt gttttcccct tgcccggcaa aagtcctgaa agactatccg gataggtgct
    25621 aggacaatcc ttctttcata gtttcttcaa cttcggcaac cgtctttgga atgggtttgc
    25681 ccagaatacg gcagccgtcc tttgtcacca gaatatcgtc ttcgatgcgg ataccgccga
    25741 agtcacggta ttcgtctacc ttatcataat taatgaactc ggtgaacttg ttggcggctt
    25801 tccattggtc aatcagttcg gggataaaat aaattcccgg ctcattggtt acgacgaaac
    25861 ccggttgcag cctgcggccc atacggagcg agctggtgcc gaactggctg gaaggctgaa
    25921 tttcggaatc gtagccgaca tatatctgcc ccagtccttc catatcgtgg acgtccattc
    25981 caagcatgtg tcccagccca tgcgggaaga acagggcatg agctcctgct gcaacggcat
    26041 cctgaacatt tccccgcatc aggccaaggt ttttcagccc tgatgccata gtttctgcag
    26101 ccttcatgtg gcagtcataa tagaaagtgc ccggttttgt gactgaaatc gatttaagat
    26161 gagaattaag gacgacttca tagatgtcac gctggcgctg gttaaatttg ccgccaaccg
    26221 gaacggtgcg ggttatgtcg ctggcataca gcagggatgt ttcggaaccg gcatccatta
    26281 ccatcatacg gccggtttcg aggtagtttt catgtttatg gttatgaagc gtttgcccgt
    26341 tgatactcag gatgataggg aacgaaaccg gccttccgtg cgatacggag atcccttcca
    26401 ttactcctgt gatctcctgc tctttccgtc ccgggaaagc catcttcatc cctgcgacaa
    26461 acatgaggta agcgatatca acagccttct ccatctctac aatttcaagg tcgtctttca
    26521 ccgaacgcag tttcacgatt ccatggataa gttccatcga agtataatcc tttaccctgc
    26581 aaggttttat ccccagtaaa ctgctgagtt gaagctgggt ttcgccgcgg taacagggta
    26641 cgaaatgaat tggacgtttg cggcggataa cattggtcat gaattctccg aggtcgctga
    26701 gcggcgccgt atttttcacg ccggctttca gggcccgttc gctgatggtc ggttgcggcc
    26761 ccatccagat aatgtcgtct atatcgacat cgtttccgaa gatataatcc acaccattgt
    26821 caatgtcgat gataccggcc aggtcaggat ggtcgaggcc gaaaaagtag gagaaattac
    26881 tatcctgcct gaacgcatac gtattgtcag gataattcat ggatgcttcg gcattgccgg
    26941 gaagcaggat tacgccgctt ttaacttcgc ggcggagggc gttacgcctg ttgatatata
    27001 tttcaggtga gaacatattg ggtttggtat tattattgca aactaaaaag gctgactcac
    27061 aaaagtaacg atttgccgtt tcatggaaca tcctttttgt gaaaatccgc ctgatgattg
    27121 taaatttgat aacccgtaat aaccgttgaa catgaaaaaa ataatcggaa ttattcttat
    27181 ggtattggcg tttggtatta ccagccaggc gcaggacacg caggctaagt cagcccagga
    27241 ctggaacaag caggggctgg aacattttaa aaaatttgag tataaaacgg cctacgaatg
    27301 tttttcaaaa gcgctggaac tcaaaagcga ctatggcgaa gcttattaca accgtggaat
    27361 gacatggttt cagttgccgg caggttctta cacctctcat gacggatgtt cggacctgaa
    27421 aaaagcaaaa gaacttggat tcaaattaaa gaaagacgat ctttctaaaa acggttgcga
    27481 atagtaatat tccgtcctga atataaaaaa gccgccttgc cggatggcag gcggcttttg
    27541 atggtttctg aggatgtaac ttattttcct gacagcataa tagcttcaat ttcatccggt
    27601 tccacaggaa tattttccat caggttaatc ggtttatcgg ctacgatcac atcgttttcg
    27661 aggcggattc cgatgccttc ttcgggaatg taaattcccg gttcgcaggt catgacggcg
    27721 ccgtattcaa agggttgctg ctttgtgccg acatcgtgca cgtcgaggcc aaggaaatgc
    27781 ccgctgccat gcatgtagta cctgaaaaat gccgggttgt cgggatcctg gtttttaatg
    27841 tcatcttcgg tgataagccc aagcccgacg agctcttttt caatcaggct gcacatggcc
    27901 ttatgccagt tgtcgttggt attgccgggc gtcagcatgg tcatgcctgc tttcagcact
    27961 ctcagaacgg cttcataaac ctgtcgctgg cgcggtgaaa atttaccgtt taccgggata
    28021 gtccgcgtca tatcggctcc gtaaagcccg tattcagcgc cgaagtccag gagcaatacg
    28081 tcgccatcct tgcagggaag gtcattgttg acataatgca ggatgcatga atttttcccg
    28141 gaggcaatga tcgggctgaa ggaatgcccg ttgcaccccc tgctgataaa ttcatggatc
    28201 atttcggctt caacctggta ctccatcatt ccgggtttca cgaatctgag gacccgtctg
    28261 aatgcatccc cggtgatatc gcaggctttt tttatctgtt ccagttcgcc gggttgtttc
    28321 gcggtacgca tcgaggtgat cagcggagca agcctttcat acgtatgcac ggggaattca
    28381 cggcgcattt tctcggcaaa acgaaggtcg cggtaaggaa cgtcggtaaa gaactttatg
    28441 tattcattct ggttcaggta aaccgtctga acatggctca tgagttcctt gagtataaaa
    28501 tcgaagtcat cgatccattt aacggtttta atccctgata atttacggac ttcatcaaaa
    28561 gtgtacttgt aaccgttcca ggtgaccatc aaatcgcttg ttttcactgt gaagagaatt
    28621 tcgcgcatgc tttcaagcgg atggcccggg cacagcacca ggatgcattt ttcctgatcg
    28681 aggcccgtga ggtaaaacag atcgctgttt tgccggtaac cgaaggtctg gtcgcccgtg
    28741 cggggcatct catcattggc atttacaatg gcaatcgagc cgggtttcat tttggcggta
    28801 agcctggccc ggttgttaat gtacatctca gaaggaatgg gtttgtatcg catgtcttta
    28861 gtatttatag ttcaaatacg gaaatatgac acataactaa tttaaatggc aatttattgc
    28921 tatatttgtt atgagtcacg gcattcaaaa ataaacaatt ccataatcct gatctctttt
    28981 tcgaatcact ttaattgttt agagtatgag taaaaaagtg cttatttatt cgtccatcac
    29041 gaagaaggtt ataatggcct tcgccgggtt gttcctgata actttcctgg ccctgcatct
    29101 gagcataaac ctgtttttac tgaaaagcga cggaggagct gcctatatga cggctgtcag
    29161 gtttatgata accaacccgg tgataaaaat catggagata ttccttttcg ggggcttttt
    29221 tattcatatc ctgatcggaa taaccattca gatatataac tggttggccc gtcccaggcg
    29281 ttacagggtg gaagggttct cccatacatc cttcttctcg aagtatatga tacatacagg
    29341 ggctatcatt ctcaccttcc tcgtcatcca ttttaccaat ttttacttta aaaagctggg
    29401 ctgggtttct cctcccgaag gtgtcgggcg cgaagacttt tatcagatgg ccatgatgct
    29461 tttttccaat agcttctatg ccatcctgta taccgttctg atgatctttc tggggtttca
    29521 tctgaaccat gcttttcagt ctgctttcca gacgctgggg cttaaccaca gcaaatatac
    29581 cccgtttatc aagggtttgg gtacggttta ttccatcgca gtgccgctgg gatttgcgtg
    29641 tatcccgctt tatatcctta tattgaaata aatgctgtat cgttagaaaa tcaaccggtt
    29701 aaccataaaa ccatttctga ttatggcaat tctcgattcg aaaacacctc ccgggccgct
    29761 tgataagaag tggagcaatt ataaggataa tatcaacctg gtaaatccgg ccaacaagcg
    29821 caaactcgat atcatcattg tcgggacggg ccttgccggg gcctcggcgg ctgcctcact
    29881 cggtgcgttg ggtttcaggg tgaaagcatt ctgttaccag gacagcccga ggcgcgctca
    29941 cagcatcgcg gcgcagggcg gcatcaatgc ggcgaaaaat tatcagaacg atggcgacag
    30001 cgtttatcgt ctgttttacg ataccatcaa aggcggcgat tatcgctccc gcgaagcaaa
    30061 cgtgtacagg cttgcagagg taagcggcac cattattgat cagagtgtcg ccaacggcgt
    30121 gccttttgcc cgggaatacg gcggtcttct cgacaaccgg tcgtttggcg gcgccctggt
    30181 gtcgcgcact ttttatgcac ggggacagac aggccagcag ttgttgcttg gggcatacag
    30241 cgctatgagc cgccaggtga aactgggaac ggttgagttg ttcccgcgcg aagaaatgct
    30301 ggacctggtc atcgttgacg gccgtgcacg cggcatcatc acgcggaacc tcataaccgg
    30361 tgaaatccat cgtcatgcgg cccatgcggt tgttctggca acaggaggtt acggcaacgt
    30421 gttttacctt tcgaccaatg ccatgggcag caatacctca gccatctgga aagcctacaa
    30481 acggggcgcc tgtttcgcca atccctgcat gacgcagatc catcctacct gcattcccat
    30541 gcatggggac tatcaaagca agctgacgct gatgagtgaa agccttcgca acgacggacg
    30601 catctgggtg cctaagaaca aggaagatgc caaagccgta aggcaaggca agctgaagcc
    30661 caacgatata ccggaagcaa accgcgatta ctatcttgaa cgccgttatc cggcatatgg
    30721 aaaccttgtt ccgcgcgatg tggcttcgcg tgctgccaag gaacgctgtg atgcaggctt
    30781 tggcgtgaac gaaacaggcc tggccgtctt cctcgatttc gccgattcca tcaaacgact
    30841 tggcagaagc accatcgaag agcgttatgg gaatcttttc cagatgtatc agaaaatcgt
    30901 caatgaagat ccgtattcaa ccccgatgat gatatatcct gctgtccact acaccatggg
    30961 agggctgtgg gttgattacg aactgcaatc caccataacc ggtttatttg ttgccggtga
    31021 agccaacttc agcgatcacg gcgccaaccg cctcggcgcc tcggcgttga tgcagggcct
    31081 ggccgacggc tattttattt taccctatac acttcagaac tacctggctg accagatcaa
    31141 agttcccctt ttcagcaccg accagccgga atttgaagaa accgaaaagc aggtccgcag
    31201 ccgcctcgaa aaactcatga acatcaaggg gcaacattcg gttgattatt tccataaaaa
    31261 gcttggccgg atcatgtggg aaaacgccgg tatggggcgt aaccgcggga accttcagaa
    31321 agcaatcagc ctgataaggg agctcagggc cgaattctgg aaagacctga aaatacctgg
    31381 agaacagaac gctatgaata cggaactgga gaaagcttcg cgggttgccg atttccttga
    31441 actgggcgaa ttgttggcat tggatgccct taaacgcgat gaatcgtgcg gcgggcattt
    31501 cagggaagaa tttcaaaccc ctgaaggtga agccttgcga aatgattcgg attttatgta
    31561 tgtttctgca tgggaatata atggcgacga caacgaaccc atcctgaaca aggaaccgct
    31621 gttttatgaa tccatcgaag taaaacaaag aaactataaa tagtcatcat aatgactcac
    31681 cggcttttgg tatcagcaaa tcatgcatca gatgtaatta tcaaccgtat tgaccatgaa
    31741 cttcacactt aaaatctgga gacagaaaaa tgcccgttcg aaaggcaggt ttgtcaccta
    31801 taaaatacag gagatttcgc caaactgttc gtttctggaa atgcttgacc agctcaatga
    31861 gcgcctggta gccgggaatg aagagcctgt tgctttcgac catgattgcc gtgaaggcat
    31921 ttgcggagcg tgcagccttt atgtaaacgg ccacccgcac ggccccgact cagccattac
    31981 tacctgccag ttgcatatgc gtaaattcag ggatggcgac actattatcg tggaaccctg
    32041 gcgtgccagg ccatttcctg tcatcaagga tcttatggtc gaccgttcgg cttttgatgc
    32101 catcatccag gcaggcggtt ttatctcggt aaacacgggc ggagtccccg acgccaatgc
    32161 cattttaata tccaaagaaa aagccgatta ttccatggat gctgcagcct gcattggttg
    32221 cggcgcctgc gtggctgcct gtaagaatgc ttccgccatg ttgtttgtgg cggccaaggt
    32281 ttctcacctg gctttactgc cccagggcag ggtggaggcc gccgggaggg tccagtcaat
    32341 ggtgaagaaa atggatgaac tgggattcgg caactgcacg aatacaggcg catgcgaagc
    32401 ggaatgtccc aagcaggttt ctatcaccaa tatagcccgc atgaaccgtg aattcctgac
    32461 tgcaaaagtc tgttcacaaa agaaaagggt aatggccgga ggaggtttct aaaaccggat
    32521 ttatcaacaa cttatacagg cttcccacag attcagggaa gccttttttt taaactttca
    32581 ggattatgtg gataaaattt gtagaattgc tgcatgaaat ttagcgagat aataggccag
    32641 gagccggtca aggcaagatt actgcaatcg gtaaaggaaa accgtgtaag ccacgcccag
    32701 ttgttccttg gccccgaagg atcaggcaag cttgccatgg ccatcgccta tgcccagtat
    32761 attaactgcc gtcagcgcac cgaaaccgat tcatgcggaa cttgtccgtc gtgtgtgaaa
    32821 tacagcaagt taatccatcc agacctccat ttcctctatc ctatcaacaa aaccaaggaa
    32881 gtagacaagg aacccctgag catcgatttt ctgccgcaat ggagggaatt cctctcggaa
    32941 aatttttctt acgtcagtct gcaggactgg tacgagaaaa tcggcatcga aaaaaaacag
    33001 gggatcatca acgccgatga cgctaactat gtgatcaagg cgttaaacct gaaatcatac
    33061 gaggcagaat acaagatcgt catcatctgg atggcggaga aattgtttca tgcggcagcc
    33121 ccgaaattgc tgaaaagcat agaagaacct gccgataaaa cggttttcat actcgtttct
    33181 gaaaatccgg attccatacc ccagaccatc ctttcgcgca cacagctcgt caaactgaac
    33241 cgtaccgggg atgacgcagt tgctgaattt ctggaagaca agctaaacct cgacgcttcc
    33301 gaggcttcat caatcgccct ggttgccgat gggaatgcga aagaagccct ggatatggcc
    33361 cgtttgtcat ccgaacagca aacaaccgga aaaggggata gcgccaatcc gaaatcagat
    33421 cccgaccggg aacggttcgg gctgttcagg gaatggatga ggcgttgctg ggttattacc
    33481 gacagcagga tcatgttaaa agattacaat ctgttgcagg aaacggttgc atcaattgtg
    33541 agcgacggca gccgggaaaa acaaaaggat ttcctggcgt atggccttaa aatgctgaga
    33601 tcctgtctgc attacaacat gggcaacagg cacctggtga aacatgcgcc atacgaaatg
    33661 gaatttattg ccaatttttc acgatatatc cacattcgta atattcagtc ttttgatgag
    33721 gaatttaatc aagcgatatt ccatattgag cgaaatgcag ccggtaacct aatctttacc
    33781 gatctgtcgc atattgtggc ccgcctgcta aaaattgccc ctccgccgca acgttaaaaa
    33841 cattaccttt gccttcgtaa gccgattaac agtgactgcc agttcattgg cctgcactaa
    33901 aatatccctg cccttgttgc gttaatcatc aacaacctgg gtgatttact atatccggac
    33961 taccctctat ggacgagaaa aaagttaacg atatattcag caataccatt cttgcacgtg
    34021 gttgttgcct tgccgggaaa accgatgacg agaataatcc cgttttcagc cacggctgtg
    34081 caaaactgga cgtgcaaaac tggctcaaag gtctcccttt cccgcctgat ttcaaggagt
    34141 ttgattgtat tgaggtacgg tttaagaaca accggaagga ttttttcagg ttgcaggccg
    34201 gtattgaagt gcacgacggt gacattgtag ctgttgaagc ttcgccgggg catgacatcg
    34261 gcgttgtgac cttgaccggg gaactggttc gtatgcagat gcgccggaaa aatgtaaacc
    34321 cggccaagga agacatccgc aaggtgtatc gccgggcacg gcaggccgac attgaaaagt
    34381 ggatgggcgc cgtggccaag gaagacaacg ctattttcaa atgccgccgt attgctgccg
    34441 acctgggttt gaaaatgaaa gtgaacgacg ttgaatatca gggtgatgat acaaaagcga
    34501 ttttttatta taccgctgac gatcgcgttg atttcaggga actgatcaaa gtattggcgg
    34561 aagaattcag gatcaggatt gagatgcgcc agatcggcgt gaggcaggaa gccagcaggc
    34621 tgggcggaat tggtacctgc ggaagggaat tatgctgttc cacatggctc accggattcc
    34681 attcggtagc tacggctgcg gcacgggtgc aacaattatc acataatccg caaaagcttg
    34741 ccggccagtg cggaaagctg aaatgctgcc tcaattatga atacgaagct tatctggatg
    34801 ccataaagaa ttttcccgat accagtatcc cgctaaaaac aaaccagggt gaagctatcg
    34861 tcatgaagat tgacgttttt gccgggatta tctggtatgc ttataaaacg aatgaaagcg
    34921 agatatttgc catacctgtt gatcaggtag aaaaaatcca gtcgttgaac aagcgcggaa
    34981 aacaggttga aaaactggag gattttgccc gcaaacagga aaagaaatca gaacttgacc
    35041 ttattgtcgg acaggatgaa ctgacacgtt tcgacaaaca taaataataa ccgacgcatt
    35101 gaaaatgccc ctgtttaaat ctttaacatc accggttaaa agctttttac aattagccct
    35161 gacattcata ccgttgatag tgctggccat tggattccat tcctgccata acaaccaggt
    35221 ctaccgcgaa acaaggcata tctcatcgga tggctggaaa cctgccgata gcatttcatt
    35281 tacttttcag gttgacgaca cccttacgcc tctgaacttt tactttaatg tccgcaatac
    35341 gacccggtat tcgtaccaga atctgttttt cttcatcaca accaaatatc cgggaggagc
    35401 attctcccgt gatacggccg aatgcatcct ggcaggaatt gacgggcgct ggctggggaa
    35461 aggcaaagga aaagtgcgtg attcaaaatt tcttttccgc aaggaagtca ggtttcataa
    35521 aacgggagaa tacacgctta ttgtaaacca ggccatgcgt gaggaattac tgaaaggcgt
    35581 gtcggatata gggctggtca ttgaaaaacc cgaagagtaa aacagtcaac ttttcgaaag
    35641 taattattgc acaattattc gcaattgaaa aagaaaaaga atcaaagtgc cagtttcgaa
    35701 gattggatca ggcggttctg gattgcattt gcagccagcc tggtactgat ggctatgttt
    35761 tttacagctt tgtcgctggg ctggttaggg tttatgccca ccttcgaaga actggaagca
    35821 cccgagagtt atctggctac cgaaattatt tccgccgatc agaaacaact cggtaccttt
    35881 tacatcgaga acaggctgaa tgttcactat tccgacctgt cgccctgggt tgttaaagcg
    35941 ctgatagcca ccgaagatgc acgttttgag aagcattcag gcatcgacgg aaaagctctt
    36001 ttgcgggtga tgtacggcat gatcacaggc accgaccagg gaggaggcag cactatcacc
    36061 cagcaactgg caaaaaacct cttccccagg aaataccaca ggacgatcat tggcaccggt
    36121 ttttcaaaac tgagagagtg gatcacggcc ataaagctgg aacgccatta ttccaaagag
    36181 gagattgccg cactttacct gaatactgtc gatttcggaa gtcaggcgta tggcatcaaa
    36241 tcggcggcga ttacgtattt cgggaaggaa ccttcggaac ttaatcccca ggaatcggcg
    36301 ctgctgatcg gtatccttaa agccccgtcg tatttccacc ctgttcgcaa caaacaacgt
    36361 gcgattcaca ggcgcgaagt tgtgcttcat cagatgatga agtaccacta tctcacacgt
    36421 gcagaatatg attccatcaa ggttttgccg ctcgatatgt cgcattacaa acttcgcgac
    36481 cataattcag gttcggcaac ttatatgcgt gaatatctgc gcattatgct gtcggcttct
    36541 aaacctcacc gtgacgatta tgacgatgtg gccgattatg tagccgattc ggtacgctgg
    36601 gaaaaagatc ccgcttacgg atggatcaat aaaaaccata aacccgatgg tgggacctat
    36661 aacctttata aggacggact tcgtatctat acgaccatcg attcacggat gcagcaatat
    36721 gccgaagctg ccgtgacaaa gcatctttct accgaccttc aaccggcatt ctttaaacat
    36781 tggaagggta atcctcatgc ccctttcgat ctgaacgata aagaagaaat caagaaattg
    36841 ctggctgctt cgcggaaacg ttccgatcgt taccgtaata tgaaaagatc aggatatccg
    36901 gccgattcca ttgacctggc tttccgtacg ccgataccga tgcgggtctt ttcatggaaa
    36961 ggtgaaattg atacgatcat gagccccgac gattccatta aatatcataa atggttcctg
    37021 caggccgggc tgatgtcggt ggaaccgcaa accggttatg tacgcgccta tgtaggcggc
    37081 attaatttca ggtatttcaa gtatgatcat gtgatgatcg gccgcaggca ggtaggttcc
    37141 acctttaagc cttttgttta taccgttgcc atgcaaaacg gagaatctcc ctgtacgcgg
    37201 gttgccaatg tccagtacac gattgactcg ggcgacggga aaatttgggc gccgaagaat
    37261 tccggcaaat acaaggaagg acaaatggtg accctgaagg aagcccttgc caattccatt
    37321 aactggattt cggcttattt gatcaagcgg tattctcccc agtcggtggt tcagatagcc
    37381 agaaaaatgg gcattgtcag tcccctgccg gcggtgccct caatatgcct gggaacaccc
    37441 gatattaccc tggccgaaat ggttggagcc atgaatacat acgcaaacga tggcatctat
    37501 attcacccta tcctgattac ccgtatcgaa gataagcacg gcaacataat ttcccagttt
    37561 gcgccgtatc aggaagaagc catgagtgaa gaaacggctt acctgatgct gatactcatg
    37621 aaaggtgttg tagaaagcgg aaccggcgcc aggttacgcg gcacctatgg ttttacaaat
    37681 cctattgccg gtaaaaccgg cacgacgcaa agcaacagcg acggctggtt catggggctg
    37741 acgcctgatc tggttaccgg tgtctgggtt ggaggggatg accggacaat caggttccgg
    37801 aatacctcgc tgggccaggg cgccaacatg gcgcttccga tttgggctta ttacatgaaa
    37861 gctgtttaca acgatagtac cctgcaattg tcaaaaggcg atttcgaaaa gcctgaagcc
    37921 ccgctctcgg tcgagattaa ctgtgcggca gcagctcagg ccggcgaggg cgatgtgaat
    37981 gattatgata aatattaaca ggcccggtca ataaagaatc aaatgtttcc tgcggatatt
    38041 caatgatctg ataacaaaaa attcgtcaat ccaacttgtc atggatcaga ggacagaaaa
    38101 aatcattgcg aatatcctga cactggcttt cctggccttt ttctgcatgc tggctgttac
    38161 ctccacagcc atttttgaag gggctgataa cattgcccat tataacctct cccattactc
    38221 attccggcat ccggaattat tgcttgacca ttggggtaaa cctcttttca ccatactcag
    38281 ttccccgttt tcacaggcgg gatttgtcgg gactaaatta ttcaatgtcc tgaccggagt
    38341 ggctgctgcc tattttgctt acaggtacct cagaagccag aaagttcaga tggcctttgc
    38401 agtgataccg ttcgtgctgt tcgtccccat gtatttcatg atgatgcaga gcgccctcac
    38461 cgaggtgttg tgcagtttca tccttgttgt gtctgctttc tgcttcgccg ataaaagata
    38521 cattctttcg gctatccttc tttcatttgt tccggtgtca cggaccgacg cggtaatcat
    38581 tcttccggtt tacgcggcgg cgctgctttg gttcaggcaa tacaaggccc ttccgttttt
    38641 gctgacaggt accttcgttt tcagcatttc aggatggttc cagtacgggg atatcctctg
    38701 gctgattcac aacaatcctt ataaaggcgc ttccagttta tacgggaaag gggatttgct
    38761 gcattttgtc gaacaaatac cctatatctg tggtattcca ttgaccatcc tgaccgtgac
    38821 gggcattttc tccattcttt tcaggttctt ccgcaataat gcgcatcccg gagaaccggc
    38881 tacagcggat gtattcgtac tgttattgcc ggcgctggcg ttgctggctg cccattcgta
    38941 tctgtggtgg aaagggcttg gcggctcggc cgggttgaca cgggttttaa cgcctgtcct
    39001 gccgttgaca gccatcatgg cctataaagg cttcgatgaa ataacctgga gaattccggt
    39061 taagtgggta agatggctta tccttctggt cgttctgttt tttatcatac tggtgccagt
    39121 cagaaaatac aggccaccag ccaaacttca aaaagctgat ctgctgatga gccagacagc
    39181 cgattggata aaatcggaag gacttcaaaa caggaacatc ttttattatg atcctgaagt
    39241 ggcttttttc aatgaccggg atccatggaa tcatgaaaaa ggcaggcaaa ttatccggga
    39301 tgggtttgta aatgaaacca ttttcgggga aggctcatta ataatctggg atgcccattt
    39361 cgggccgcac gaaggcaggg tacccttatc tttgctgctt gaaaacaagc agctcaggct
    39421 gataaagata ttgcttccca atcctccctt tacgatttat ggaaatgaag aatactgcat
    39481 atatgttttc ctgaaagacc gaacacatgc cccgcatgat aattttgcca tcgagaagga
    39541 gttgcgcagt actcattttt aacctgacca atctgccatg cagatgatgt tcacactctt
    39601 atgaagaata ccgaaacaga tacagaattc atgatgccga aacgaaaaag ccggttgtcc
    39661 ctgattgcat gttggctgat cattttcctg gttgtcgggg caaatctgaa atatcgtcac
    39721 tgggaaaacc ggtataaaat catttatgtc gatgtccttc actattatgc ttatctgccg
    39781 gccactttta tccatcacga tctttcgctg gaattcatcc gtaccaacaa ttcctattac
    39841 agccagtatt tctggcccag agttctgcct gataaaaagc tgatcatcat gaccaccatg
    39901 gggatgtcgt acatgtacgc tcccttcttt ctggcggttc attacccttt gaaatggctg
    39961 ggggagcccg cactgggata ttcccctccc tataaaatgg ccctgatagc aagcagtata
    40021 ttctggtttg cccttggcct cttttttcta aggaaactgc tgcttaaata tttttccgat
    40081 ttcacgacat ccctcacgct tatcatcacc gcgctggcta ccaacctgtt ctattatgtt
    40141 accgacgagc cggccatgtc gcatgcctat ggttttgccc tgtttgcgat gtttatatac
    40201 tttaccgacc gttggttcga aaaaccgaag ttcgggtact cgctggctct cggggtgctg
    40261 gcaggggtaa tagcgctgat caggccttcc aatgctgtaa ttgtgctgat attgttgtta
    40321 tggggcgtcg attcctttca gtcgttagga aaaaggctgg ggctgtttct ccgccaatac
    40381 cgtatggcgc tcgtcatggt tgcagcggca atcctggtat gggtgccgca ggtgctgtac
    40441 tggaaatatg caagcgggca ttattttgtt tttacctatg gagaaaaagg aaaatttttc
    40501 ttcaacaacc cgcagttctt cagggtattg ctgggtttcc gcaaaggctg gctgatctac
    40561 accccggtga tgatcttttc aattattggc ctgatacctc tctggaaaaa gcacaggaac
    40621 cttttctggc cggttgtgat atttgcggct gtcaaccttt ggattgtttc atcctggtgg
    40681 ctgtggtggt acggcggcag ttacggcatg cgggcgctga tcgaatccta tgccgtgatg
    40741 gctctgccat tggcggcctt cattgcctgg gtaacggaaa acaggagtat actggttaaa
    40801 gcggctgtag ccgtcctggc tttagtgttc gtaacccata acttcttcca gattacacaa
    40861 taccatacgg gcgcaattga tgcaataaag atgacgcggg aagcctattt tgcatcattt
    40921 ttaaaaagga atccaaatac acggcagcag agattgctgg tttatcctga ttataaagct
    40981 gctgaatacg gccggtatcc caggccaagt tctgtcagag attctttata ttccggttta
    41041 ctcgaccggg aacatgccgt caggaaaatt gaacaggaaa tccgctccgg cgaagaatcc
    41101 atgaagatga tgcagcaaaa ggctttagcc aggaatgtca gcattgattc agccatttca
    41161 attgatgcca actacctgta cgatttaaaa gtgaagaaag gcgagatacc tgcaaatccg
    41221 gggaaattta agatatttga ctggtggtac taaggtgacg ggatgatcag gatagatgaa
    41281 aacgatgcta caataaacac tgttggaatg acacaaactt atacttactc agtcattatt
    41341 ccggcttata atgaggaaac agcgatcggg gatttgctca atcgcatgac ggaacttgga
    41401 ataaccgaaa aatacgaagt aatagtgatt gacgacggct cgaccgatcg cacctgtgaa
    41461 attgtgagcc ggttccctgt gaggcttatc aggcacgaaa ccaataaagg gtacggggca
    41521 tccctgaaga cgggaatccg tcatgcttcg gggcaaaaag tcatgaccct cgacagcgat
    41581 ggtcagcatg atcccgccta tctcagcgag atggtaaaac tgctggccga gtacgatatg
    41641 gccatcggcg aacgtacggc cgattctttc caggtgaaaa acaggagggg agggaaaaaa
    41701 atcatccgta aagtgggaga atatcttgtg gaacagaagc ttcccgattt taactcgggg
    41761 ttcaggggct tcgaccggca actcattttg tcgatgcttc acctgatgcc caatggattc
    41821 tccttttcaa ccaccagcac gctggctttc ctgaaggaag gatattcgat cggcaaattt
    41881 cccataaagg tgaacggccg gcagggaagg gcaagtagcg ttaaatttat taaagacggc
    41941 actaaaacca tgttgctgtt attccgtatc atcatgcttt tcaatccgtt gaaagtcttt
    42001 tttccggcaa gcgctttcat aacaatggtt ggcgctatct ggggcattca tggctatatc
    42061 gtattcgacc gtttctcaaa tacggccatt atagtcatca ttctgggaat gtttcttttt
    42121 ttcttcgggt tgcttgccga ccaaatcgca ttacttaacc gaaaaaaagc ttgacggatt
    42181 tttcaaaata tcagaattgc tggtccgacc gggacgtgga ggcacactgg gaccgtgttg
    42241 ccggccggta tgtggaagag aatgataaag tgaaacaaac ccatgaccag cgcttttcct
    42301 tcagcctccc atacctcgac ctgtattgcg gggccagggt gctcaacatc accagccgcg
    42361 acggcgaagc cgctgcatac atttccgcga aggaaccttc ggtggaggtt acaaatgctg
    42421 aaatttccca aaaactgatg gatgttgccc tgaaattaaa tccttccatc cgccaggtga
    42481 aaatcagcac ctattcgcac ctgcctttca gtgatgaaca attcgacagg atacttacgc
    42541 ttgaaaccct cgaacatgcc gccaatccga tggagtttct cagcgaattg caccggattt
    42601 cgatcccgga tgcacgcatg gtgttgagtt gccctcctgc taccagtgaa attccctatc
    42661 gcttttatac tgccgttttt ggcgggcacg gggaaggccc gcacaggttt cccccttcaa
    42721 aacaagtgaa agaatggctg aaattatcag gctggaagct taccttccat aaaggaacgg
    42781 tactgatgcc tgttggtcca ggatggttta aaagcctggg cgaaaagttg attgataaaa
    42841 cccagggaac ctttatttcc gaattgggaa tacgacaatt ttacgtttgt gaaaagtatt
    42901 gagaacctgc aacatgtaat acgccacaac ctgtgcaacc gatgcggtac ctgtgtcgga
    42961 ttgtcgggcg gaagaattgt attcagcgat aagactggta aatatttacc ggaaattaca
    43021 gcagaattgc cccgtgaact tgccgaccgg atatgggaag cctgtcccgg acagaaattc
    43081 gatttcccgg agtaccgaaa gcttttttac ccagatgcgc ctcattttca tttatatacc
    43141 ggtccgtacc gggatctttt catcggttat gcaaccgatg agcttacaag gcggaattcg
    43201 gccagcggag gtattttaac ggcggtactt ctatggctgc tcgataagaa atggatcgac
    43261 ggggctgtgg ttaccgccat gtcggaaaag gaaccctggc tggcccagtc ctttatcgca
    43321 accacggccg aagaaatctt aagagcggcc cagagcaagt atatcattac ttctgtgaat
    43381 gaaatactgc cgcaaataga ggcctttcag ggaaaactgg cttatgtcgg gctgccgggc
    43441 caggtgcaaa gcatccgaaa gctgcaggcc acgcatgatg tttcggtgca gaatatccgg
    43501 tatgtcttcg ggccttttta cggaaatacc cttcattttt cttcaataaa gagtttcctg
    43561 cgttcctatg gcgaaaagga ctttcatgat atcagccggt tatggttccg gtatggggaa
    43621 tggcccggca acatgcgcat cgaaatgaaa agcgggcggg ttatccaatt accgaaattt
    43681 catgccaatt acctgatccc ttttcatatc ctgaaaaaca gcctgttatg caccgatctc
    43741 agcaatgagt tcaccgatat ttcgggcggc gatgcctggg cgccggttta tgaagagagg
    43801 ggcaaaggct tctcgatggt tttttcacgg agcgggcaag gaaaagaaat cctggaacaa
    43861 atgcagcgag aaggcgtcat cagcctgcag cccatcgatg agaacgaagc cataaccatg
    43921 cattcacatg gctacgacct gaaaaaacga ggttcgttca tcaggatgaa attcaggaaa
    43981 ctggcaggat tgccgaatcc tgattacgg
//
LOCUS       DAOPYV010000020        43732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_726_length_43732_cov_12.232754, whole genome shotgun sequence.
ACCESSION   DAOPYV010000020 DAOPYV010000000
VERSION     DAOPYV010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 43732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43732
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_726_length_43732_cov_12.232754"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..1277
                     /gene="trxB"
                     /locus_tag="PKI35_04095"
     CDS             336..1277
                     /gene="trxB"
                     /locus_tag="PKI35_04095"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNX43595.1"
                     /translation="MNSEIEMTECLIIGSGPAGYTAAIYASRADLKPVLYTGLQMGGQ
                     LTTTTEVDNFPGYPEGAKGPDMMEDMRRQAERFGADIRFGIISQVDFSRRPFSVTTDD
                     GKTVEAETVIIATGASANWLGLESETRYNGHGVSACATCDGFFYRGLDIAVVGGGDTA
                     CEEASYLAKLGRKVYMIVRRDELRASKAMQHRVMTAPNIEILWNSSTKEVLGDGKVVT
                     GALISNNKTGELRQIEIQGFFVAIGHKPNTDVFRGQIELDEMGYIKTIPGSTRTNVEG
                     VFAAGDVQDHVFRQAITAAGTGCMAAIEAERWLLLNK"
     gene            complement(1343..2281)
                     /locus_tag="PKI35_04100"
     CDS             complement(1343..2281)
                     /locus_tag="PKI35_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX43596.1"
                     /translation="MFLVSSHNLFLTSQTGNEPWLLARLILAAAMVLAACLIFMYFRQ
                     RRKLKGILRENSLLVDEMERLKEEIHIVRQQAGESDRLKSAFLCNMSHEIRTPLNAIM
                     GFCGLIANHTLPDTDKVQYASIINRNVDSLLELINDIFDIAQVESQITRVEEKPVKIN
                     DMLGSLQSWFNLEKSTLGKEMVQFRIEKANKDNDFAIQSDEYMLRRTLNNLIENALKY
                     SEEGYVDVGYRFGRDSKIDFFVKDEGIGFSKDKLDIIFKQFRQVDETQTRQYGGLGLG
                     LTVAQKFVELMGGEMWAESELGKGSTFWFSIPYKKA"
     gene            complement(2467..3108)
                     /locus_tag="PKI35_04105"
     CDS             complement(2467..3108)
                     /locus_tag="PKI35_04105"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNX43597.1"
                     /translation="MDKFHYNYIAIEGNIGAGKTSLATRLSAETGARLILEQFEDNSF
                     LPKFYEDQERYAFPLELSFLADRFQQLKTGVGSHDLFSPFTVADYFIHKSLIFARKTL
                     NNDEYMLYSKLFSIIDSVLPKPELLIYLYSSVDRLQTNIRKRGRSYEQKIAPEYLQKI
                     QDGYLDYIRQQKEMRIVIIDTNLIDFVENPGDYPKLKEIILSEYPVGIHRITP"
     gene            complement(3158..3658)
                     /gene="folK"
                     /locus_tag="PKI35_04110"
     CDS             complement(3158..3658)
                     /gene="folK"
                     /locus_tag="PKI35_04110"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNX43598.1"
                     /translation="MAIVYLSTGSNLGDREAALSQALTALNNQAGKVIRCSSIYETEP
                     WGFNAELNFLNQVLILRTSLTPDMLNATIHGIEKEMGRSRNSPAYESRIIDIDILLYG
                     RQIISREGLLIPHPRLHQRKFVLVPLAEISPGRIHPVLKRKISSLLESVDDECTVRVW
                     KKAAEI"
     gene            3847..5616
                     /gene="sppA"
                     /locus_tag="PKI35_04115"
     CDS             3847..5616
                     /gene="sppA"
                     /locus_tag="PKI35_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNX43599.1"
                     /translation="MKQFLKMTFASMLGFILAGLLIFVIFLGIVASALSFTKKQVVEV
                     PANTVLQITLDQPVNDRASEDPMSYLNFRDMTANKPMGLTEILESIKRASADKKVSGI
                     FLDLAEIPASIATIEEIHNSLLDFKKSGKFIISYSEGYNQKAYYIASVSDKIYLNPAG
                     SMEFKGLSGQVMFFKGLLEKLDIEAQVIRHGKFKSAVEPFILDKMSEPSKVQTLSYVS
                     AIWDHMLQGISDARKLPAADLTAIADNHKIQTPEDAVANKLVDKLMYKDQLLDELKAR
                     VSAKSVKDIHVMKLSKYMDAPKPEKREALAKDRIAVIYAQGSIVSGNGDDQNIGSERI
                     SKAIRKARMDEKVKAIVLRVNSPGGSALASDVIWREVTLARKEKPVVVSMGDLAASGG
                     YYISCAANKIFASPNTLTGSIGVFGIVPNMQKFFDKNLGITFDEVKTNPYADYIPTTR
                     PMTEAEKGFLTNDIEHIYATFLSHVAEGRHMSVAAIDSIGQGRVWSGTDAKRLGLIDE
                     FGGLKDAVAEAAKMAKIEKYRIISLPEQKDAFTQIIEAFSGDETSVLKKELGAAYPYL
                     KYLQNMSRMEGVQAMLPYDIRIE"
     gene            complement(5758..9297)
                     /gene="nifJ"
                     /locus_tag="PKI35_04120"
     CDS             complement(5758..9297)
                     /gene="nifJ"
                     /locus_tag="PKI35_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNX43600.1"
                     /translation="MTEKKNFVTCDGNYAAAHIAYMFSEVASIYPITPSSTMAEYVDE
                     WAAFGKKNIFGETVRVIEMQSEGGAAGAVHGSLQSGALTTTYTASQGLLLMIPNMYKI
                     AGELLPGVFHVSARSLAAQALSIFGDHSDIYATRQTGFAMLSTGSVQEIMDLAAVAHL
                     AAIKSRVPFLHFFDGFRTSHEIQKVEAPTAESLAGLVDYDALKAFRNRALNPELPKTR
                     GTAQNPDIYFQSREAANPFYLALPDLVEEYMQQMKKVTGREYHPFTYYGAPDAENVIV
                     AMGSITETIKEVIDHLNAKGEKLGLVTVHLYRPFSTKYFFKAMPESVKRICVLDRTKE
                     PGANGEPLYLDVREAFYERDHKPLIIGGRYGLSSKDTTPAMIISVFNNLKLNEPKNRF
                     TVGIVDDVTFTSLPVLPEIDLADAGTFQAKFFGIGSDGTVGANKNSIKIIGETTDKYC
                     QAYFAYDSKKSGGVTTSHLRFGDKPIRSPYLVNTPDFIACHVPAYINKYDLLKGLKKG
                     GTFLLNSIWDAEETRKRLPDSMKKFLAENEINFYIINATAIAEEIGLGNRTNTITQAA
                     FFKISGVIPYETALEEMKKAVKKSFGRKGEDVVKMNIEAINRGAAVTRVEIPAEWKSI
                     IVKIEETVQNDVPEFIRKVVEPINAMQGDLLPVSAFKGREDGTFPAGTTAYEKRGVAV
                     NVPEWQAENCIQCNQCSFVCPHAAIRPFLLNEDEMKGLSAGTKTLKAIPKTFDGLQFR
                     MQVSPLDCTGCGNCADVCPAKTKALVMRHLDTQLHQADNWDYMTKKVTYKDTLAPKDQ
                     NVKNSQFAQPLFEFSGACGGCGETPYIKLITQLFGERMMVANATGCSSIYGGSAPSTP
                     YTVNKASGCGPAWANSLFEDNAEYGLGMALGVGKLRARIAGRMESILEGNYSEELKEA
                     CKEWIAGKDKAASSQAATLKVLPLLEKESDGLCKELVSLKHYFVKKSVWAFGGDGWAY
                     DIGYGGLDHVLASGEDINVLVLDTEVYSNTGGQASKSTPAGAVAKFAASGKRVRKKDL
                     GMMAMSYGYVYVAQVAMGASQSQYLKAIREAEAYPGPSLVIAYSTCINQGLHSGMEKA
                     QDQQEKAVQAGYWHLYRFNPELESQGQNPFQLDSKEPDWTKFQDFLKSEVRYTSLMKT
                     FPTEAAELFATAEKNAKWRYNTYKRLSETDFTK"
     gene            complement(9634..10338)
                     /locus_tag="PKI35_04125"
     CDS             complement(9634..10338)
                     /locus_tag="PKI35_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43601.1"
                     /translation="MTPVEEENFAKELAANKEMAAEFRFSQQIDNALKRDDIIDVRRK
                     VNYLYEQQKASYSHNRVIHMHSRRWYFAAASVVAVLAIGATLLFSLPNRTSNDALFAQ
                     YYSSEKILDVTRSGDANIVEAIMKFQEKDYNMAAWQFGRILEKDESNMAVWFYYGISS
                     IETKNYTKAISAFEKIIRDNRNLYVEHAEWYLSLCYLKSDQTDKAINQLKLIAANDNN
                     FHQQEAIKLLSKIAKE"
     gene            complement(10392..10970)
                     /locus_tag="PKI35_04130"
     CDS             complement(10392..10970)
                     /locus_tag="PKI35_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX43602.1"
                     /translation="MIHYSDEAIVEGLRLRSDYIITFVYREYFPLIKYLVNENSGADE
                     DAEDIFQDGLIIIYKKITDNQLTLTSSFKTYMYSVCRNLWLQKLNKRKTVMEKISDVE
                     EYIDLPEDIMHEISNEEIELHRLVQLHFLSLNEDCQKILRLFMKDVSLREIGNIMGFK
                     TEKYAKTRKYMCKEELKKRIANDPRCQKFFND"
     gene            11431..12654
                     /locus_tag="PKI35_04135"
     CDS             11431..12654
                     /locus_tag="PKI35_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX43603.1"
                     /translation="MKKTYRNFLVLVFTLLLGTASASTVSGVLLYLDNPQNPIPNTVV
                     KLTNLSTQSVMTVVTDDNGNYEFTDVVPGNYRLSATTSLASGGVDLLDALEVLKYLIG
                     QTSFTQIQLLAADVNGSGSVKLSDFVLIMNHLLHGTPFPVGNWVFETFNFTVSGTKEE
                     PGGLSGSSSGDLTGVFVPGGRNLPAFPVEHNAKIAATAENEIEIPLITYDALELTGAG
                     LIFNYPGNLISIESVEFPGNDFQYAISGDQLKLYWYGESDAPVSFSAGSALATLHCRT
                     TAAFAPGTEADFSLDGSTCLAGGNYTKITGAKLGIPTITYAAPSLRISNYPNPFAEST
                     MITYYLPEDGNVTIRIYSYTGQLVLTMPLSDRKEGYNQVNLDAANWLPGNYICRIQSN
                     GSRNYNESRILIKTN"
     gene            12794..16138
                     /locus_tag="PKI35_04140"
     CDS             12794..16138
                     /locus_tag="PKI35_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNX43604.1"
                     /translation="MQLPLLLRKCKNASLITITGVACHVLIVILLLSGCSGNHNDKRP
                     VNQGGDLETGEALMQQSLFDSALVYYNRVARNGLKTGSWDIWYKGTAGLIDCYRAKGN
                     FEEAFRIANQAQQIASGRGDTSDAIYAGILHKKAVILTDKGDYTTSSSVLSESILIRS
                     RGKENADTAMALSYNILGNNNLFQGEYDKALENYLKAITLFKDRKNSADLATINQNAG
                     IVYALKGDYDNAKNFFHEALTINQAVIPANDPKLALMYLNYGRYYSLIENDREALEMY
                     NKAESIFRKSGDKSTKNLAPLYHNIGNIYANYADFDKALLYFNKALSLYQSHPSEGDQ
                     NISAILLNIGYIFEKEGNYQQALKYYLQSVEKGKNTQNKVTIYRNLANIYYIIKQPEN
                     AFLYYDLALDASLSGFGPDHPETALTYLKFGEFLSLTGRDHEAITDLNKGLQVYQKLF
                     GSKNPQVAAAYKYLGNHYTRKNNFEKALNCFQQSLIAGFANFNKTEAAYNPAISKSEV
                     NYFMLNSLSGKAFALRSLYLKNHSRTDYLVSSADAYDVSICTIEMLRSSYQDEDSKLL
                     MAGNEKWIFQNAIQVNSELYNRYRDKKYLIKALELSEKSKSAVLLSNIRNLEAKEVGK
                     IPAKLRIQENKLKEEIGLYEKLVYDEKSKANADAQKINFWNNKIFELNKWYDSLIVIF
                     EKEYPAYYKIKYDNSVLGLSEIQRKLKDNQAVIEYTMDDESYLFAFVITRRYCTLKHI
                     PLDSLFFSDINTLTGQMAGKSFNNFSQDDFTSFVSASRRLFEKLIPEEISEHLPPDLI
                     IIPDGELGYLSFDVLLTEKPSDQASGYRNLPYLIKKCAINYAPSATMLFGELSNKGKP
                     GHDVLAFAPSYDNLKGIPINLMMNRQSAQNYLLPIPGAQIEVDNLKKIFNCKVYQNNK
                     ATKDNFRKFAGDYSILHLAMHTLIDDKNPLYSKLVFYQDKKPDDNGLLNAYELFNMQL
                     KAQLAVLSACNTGRGKLVSGEGILSLSRGFFYAGVPSIIMTLWAVEDRSGADLMTSFY
                     HYLSEGKAKNEALRLAKLDYLKSSDQIRSHPHFWAAYLSIGNTDPVQDIGKPVSSAKY
                     ILGSAFILAVVVATFWAYRRRRRSSL"
     gene            16190..18616
                     /locus_tag="PKI35_04145"
     CDS             16190..18616
                     /locus_tag="PKI35_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNX43605.1"
                     /translation="MIYPGNFEEKIGFDVIRGLLKDKCLGPAGKRLIDAITFATRFQD
                     IDPELLVTGEFRELLLTVPGFPMSDYLDLDEELQRIRTAGTFLIPENLVQLRHSLSVV
                     SDVLNFIRSLDSYSFPATIRLAEDVFVDGRIISEINRILNEQGEIRDNASEKLFSVRK
                     SIVSLRSQIDRRIAQSLTQAKTQSWISEEAQITIRNGRLVIPVPAANKRRIKGFIHDQ
                     SATGQTVYIEPADVFELNNDIRQLELSEQQEIVRILIQFTDFLRPGLDNISIAFDFLG
                     RVDFLRAKAVFALETGGTKPFFRNDRKIDWIEARHPLLFLSHKRLNKPVIPLDIRLDE
                     ENRILIISGPNAGGKSVCLKTVGLLQYMLQCGLLVPMKETSETGVFTDLFIDIGDQQS
                     IDNDLSTYSSHLINIKNLLSFAGKSSLFLIDEFGAGTEPQSGGAIAETVIESLNQKMA
                     FGVVTTHYANLKLLASKGSGIVNGAMLFDTRKMKPLFKLSIGKPGSSFAFEIASNIDF
                     PAEILSKAIEKAGTSKLNYDKLIQGLENERAEISLKEKQLQHADDLLASLISKYETLK
                     SDLEKKRKSILEDAQAEARHMIADSNTLIEKTIREIRQSQAEKSKTRQLRQEITEMAA
                     KLETDASPDRESIPATAPESQPEPVVKAFPEIGDPVKIEGQSEIGEIEEMTSADALVA
                     FRTVKLRVPLNRLIPFEKTGDKPKGKSAYDYRYHNIVNEINARLANFRLSIDVRGHRG
                     DEAVALVQKYIDEAILLNVPEVSILHGKGNGVLRRMIRDYLHTVSEVRQMTDESLERG
                     GAGITVVRFR"
     gene            18648..19061
                     /gene="trxA"
                     /locus_tag="PKI35_04150"
     CDS             18648..19061
                     /gene="trxA"
                     /locus_tag="PKI35_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNX43606.1"
                     /translation="MMKNRLSVTLVITLVCSFAGSCSDGKRTDEAPDTILLTVDNFEK
                     EAGKDVVMIDFWAAWCKPCRGFAPTVEKIAGQTAGKVKVGKVDIDAQDELSNKFRIYS
                     VPTVIILKDGAEVERFIGVQPEGILMKALGKYTEF"
     gene            complement(19062..19409)
                     /locus_tag="PKI35_04155"
     CDS             complement(19062..19409)
                     /locus_tag="PKI35_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNX43607.1"
                     /translation="MNIEDLRDYCLIKKGVTEGFPFDQSTLVFKVAGKMFLLTDLTDA
                     FSITVKCDPDKAPGLREKYPCVTPGYHMNKKYWNTVNINGSIGDELILEWIDDSYRLV
                     VGSLPVKERKSFL"
     gene            19628..21175
                     /locus_tag="PKI35_04160"
     CDS             19628..21175
                     /locus_tag="PKI35_04160"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNX43608.1"
                     /translation="MSLSTKTTDLKRRMREALRGGGEKAIEKQKATGKMTARERIIAL
                     LDPKSFHEYDLFVEHAGRDFDMSDKYLPGDGVITGTGTINGYPVCIFAQDFTVAGGSL
                     GYMHAKKITKIMDHAMKLKVPLIGINDSGGARIQEGVNSLAGYGEIFWRNTQASGVIP
                     QISVILGPCAGGAVYSPALTDFVFVVDNISKMFITGPEVIKTVLGEDVSMEELGGARV
                     QAEVTGNAHFFAESEQECFEQIKQLCSFIPWNNSKKADPFPVKPPRTRQYNINKIFPT
                     NPRQPYDVKEIICAIADDSDFFEVHGRWAENIVVGFGRINGHTVGFVANQPLVLAGVL
                     DVDSSDKAARFIRYCDAFNIPLVTLVDLPGYLPGVDQEHAGVIRHGAKILYSYSEATV
                     PKITVILRKAYGGGYIAMCSSHLRADFVLAWPTAEIAVMGPEGAANIIFRNEIKNAEN
                     PDEMRKQKIKEYKDKFANPYVAAAYGYVDAVIEPEETRNMVIHALQISLEKGIERPTK
                     KHGVPPF"
     gene            21291..21656
                     /locus_tag="PKI35_04165"
     CDS             21291..21656
                     /locus_tag="PKI35_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNX43609.1"
                     /translation="MEEKSKEQQINPLPEEPQAEEVLQSLTIEDVRYKTRLNKKFLAR
                     KNYQPRDPKEIIAFIPGTVINVFIKKGSKVKQGEKLLELEAMKMVNTLFAPADGVVKD
                     LLVKTGDLVSKNQVLMHFK"
     gene            complement(21660..22283)
                     /gene="yihA"
                     /locus_tag="PKI35_04170"
     CDS             complement(21660..22283)
                     /gene="yihA"
                     /locus_tag="PKI35_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNX43610.1"
                     /translation="MSTQPASYVMSANDRNGCPHERYPEYSFVGRSNVGKSSLINMLT
                     GVKGLARTSATPGKTRLLNYYMVEDSWYLVDVPGYGFAKISKTERERWEHLIHDYILH
                     RQSLLYTFVLIDSRLEPQKSDLAFITWMGQHVLPFCLVFTKTDKISHNKVHSNVAAFR
                     KELEKDWEELPPMFITSSLSGKGKAEILEFIKKCNQEFGPLISEARQ"
     gene            complement(22365..23297)
                     /locus_tag="PKI35_04175"
     CDS             complement(22365..23297)
                     /locus_tag="PKI35_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX43611.1"
                     /translation="MLEIIPPVDRKLIIDELTPDKFVKYTNFGNNEIYIVTWHNSPNV
                     VTEIGRLREITFRDAGGGTGLDCDLDQFDKDPDVPFKQLFVWDPNEQEIIGGYRFIHG
                     KELRIRPDGQVDTPTTELFTLSDTFVKDYLPWTIELGRSFVQPAYQPNIDVRKGMYAL
                     DNIWNGLGAIIVQNPDVKYFFGKITMYPHYNTRARDLILHFMNKYFNDHKNLVYPKQE
                     LELETPVEDLESFFTAGQYDKDYRLLVQEVRRCNENIPPLVNVYMSLSSTMLCFGTSL
                     NPGFGNVEETGILVTIDDVYDIKKERHITPSPRS"
     gene            complement(23320..24186)
                     /locus_tag="PKI35_04180"
     CDS             complement(23320..24186)
                     /locus_tag="PKI35_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNX43612.1"
                     /translation="MDDVNNGTEITPGFIDLERVIKSKSESLNKALPRFIKSYLKRII
                     HQDEINKALYRNRDKTGLDFIAAILTEFGAKLNFTGAENIENTPRFIIASNHPLGGLD
                     GLALMHVAGKFRPDIVFPVNDILLNLPPIKNLFIPINKHGSNAENAKIIDDTFASDKS
                     ILYFPAGLCSRKQSGGISDLEWKKSFITKARKHRRDIIPCHIDGRNSNWFYNLSRFRN
                     RLGIKANIEMLYLVDEMYKQHDKTLTIIFGKPVPWQIFDKRHTDLEWAQLMKEHVYRL
                     EKTPDLTFEESI"
     gene            24483..24944
                     /gene="mraZ"
                     /locus_tag="PKI35_04185"
     CDS             24483..24944
                     /gene="mraZ"
                     /locus_tag="PKI35_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNX43613.1"
                     /translation="MPDLIGVHECKADAKGRVLFPVALKNQLLDVLEQGFVIKRSIFQ
                     QCLELYPRPEWNMVSRDVNKLNRFVKKNNDFIRVFMAGVREVSLDGTGRIQIPRDLVN
                     FAGIKNDIVLASAVNIIEVWDKDKYEAAIGSSMEDFSTLAEEVMGSISPTE"
     gene            24952..25839
                     /gene="rsmH"
                     /locus_tag="PKI35_04190"
     CDS             24952..25839
                     /gene="rsmH"
                     /locus_tag="PKI35_04190"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNX43614.1"
                     /translation="MYHLPVMLSESIEGLAIKPGGIYADLTFGGGGHSRAILSKLNDQ
                     GRLIAFDQDEDALNNAIDDERFLLINQNFRYFRNFLRMHKALPLDGILADLGVSSHQI
                     DTPERGFATRFDGPLDLRMDRNAPFTAEQLLNSYPEEKLKSVFAEYGEIANARQLAYV
                     ITQSRPLATIAEFKKAIASCIPRKTENKYLAMVFQALRIEVNDEMGVLKAMLKQTAEA
                     LKPGGRLVVIAYHSLEDRLVKNYIRTGNFEGVPEKDFYGNLQSPFLAITRKPLTPGAG
                     ELESNPRSRSAKLRIAERK"
     gene            25842..26240
                     /locus_tag="PKI35_04195"
     CDS             25842..26240
                     /locus_tag="PKI35_04195"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HNX43615.1"
                     /translation="MDEPKNQLNIPSKEEDPKPEKKAKRKFSLKVKNILDGNFLTRER
                     VIGLLPYLGFVTMLGVVLIFNTNYADKTIIEIGKTKRMNEELRYEYIVTKSKLMQLSR
                     QSQLVKTIASTGLKESTVPPRKILIEKAEE"
     gene            26266..28356
                     /locus_tag="PKI35_04200"
     CDS             26266..28356
                     /locus_tag="PKI35_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HNX43616.1"
                     /translation="MTDTKKDILWRVYLVYIGMFVLGLTIIGRIVYIQVAEGDKWREK
                     ARKLSLRYFNIEATRGNIYDANGEFLSTSVPIFEVRMDVASPYITDDIFKKEADSLAY
                     RLSDFFKDRSKREYKTLITRGRAEKNRYLLLKRNVNYDQLKTLKTFPIFRNGKNKGGL
                     IAKAVERRIQPYSHLAFRTIGWDKEGKQNDLGLEGAYSDELEGESGKRLMQRIANGNW
                     RPLNNENEIDPRNGSDIVSTVDIHLQDVAEDALMKQLIANDADHGCAILMEVKTGRVK
                     AIANLGKNADGQYEEKYNYAIGEASEPGSTFKLYSLLAALEDDKTDLDEKIDCNGGIA
                     YYANRAMKDSHLGTGVLTVKQIFEKSSNVGVSKVIYKAYASNPQAFIDRLYSFSVNKP
                     LNLEIAGEGKPRIKDTHARSWSKVSLPWMSIGYEVALTPLQILTFYNAVANDGVMIKP
                     RFVDEIRLSDKVIKKFGPVIINPKIASKKAIHKAKIMLEGVVQEGTGTSLRNPVYKVA
                     GKTGTAQIAMNNAGYNKSSYKGTFVGYFPADNPKYSCIVVINNPTKGVYYGGAISAPV
                     FREIADRVYAADMEMGLTWDDSVKITHRVPFKGGFGRDMATICRWMGYNMQHYGNNWI
                     TSDSSGRKTVAMNYKSGIVPDVCGMGMRDAMFLLEKQGYKVRATGVGLVRRQSLQAGT
                     RARAGSLVELNLDI"
     gene            28376..29827
                     /locus_tag="PKI35_04205"
     CDS             28376..29827
                     /locus_tag="PKI35_04205"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNX43617.1"
                     /translation="MKQLSEILSDVKTLSVKGNRDVMAGDVTFDSRKVKAGSLFVAVK
                     GTHTDGHDYIPAVISAGAVVVVCERLPETLDPKVTYVQVADTSFALGQIASAWYDNPS
                     SNLKLVGITGTNGKTTTVTLLFNLFREAGYHAGLLSTISNRIDDEEIASTHTTPDAVE
                     LNSLLSQMVDRGCDYCFMEVSSHSIVQNRIAGLTFAGGIFSNITHDHLDFHVTFDEYL
                     KAKKRFFDELPSGAFALTNLDDRNGRVMLQNTQALKKSYSLMTMADFKGRIIETPLDG
                     LHLEIDGKEIWCRLVGQFNAYNILAVYSAAVLLGEEPFDVLTILSRLGSVEGRFDYMK
                     SSDGIIAIVDYAHTPDALQNVLDTISNLRTGNETLITVVGCGGDRDRTKRPIMAQIAG
                     RMSDKVIITSDNPRSEEPEAIISEMLAGIEGEYSRKVIKITDRREAIKTACAMARPGD
                     VILVAGKGHEKYQEINGVRHHFDDKEQLREFLQ"
     gene            29869..31122
                     /gene="mraY"
                     /locus_tag="PKI35_04210"
     CDS             29869..31122
                     /gene="mraY"
                     /locus_tag="PKI35_04210"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNX43618.1"
                     /translation="MLYYLFTYLDKAYDVPGAGMFQYISFRASCSAILSLIISLLYGK
                     RIINYLRTKQIGETIRELGLEGQSEKAGTPTMGGLIILGAILIPTLLFARLDNVYVLL
                     MLFATIWLGLIGFIDDYIKVFKKNKEGLAGKFKILGQVIAGIVIGCVLYFNGSVVIRE
                     KLPEPKIRTTSEITAFERESSHITKFSPTPAKSLKTTIPFFKNNEFDYSVLIGWAGEK
                     AGKYAWIIFIVIVTFIITAISNGANLTDGMDGLATGTSAIIGITLGLLAWVSGNIVFA
                     DYLNIMYIPRVGELVIYISAFVGACTGFLWYNSYPAQVFMGDTGSLALGGAIAAFAFM
                     IRKEFLIPILCGIFIIENLSVVMQVAWFKFTKKRYGEGRRIFLMAPLHHHYQKLGYHE
                     SKIVLRFLIVNIMLAILTFITLKIR"
     gene            31128..32468
                     /gene="murD"
                     /locus_tag="PKI35_04215"
     CDS             31128..32468
                     /gene="murD"
                     /locus_tag="PKI35_04215"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNX43619.1"
                     /translation="MSRKIAILGAGESGVGAAILAQSKGMETFLSDNGKIKEKYADVL
                     SNSGIAYEEGKHTIDRILDADEIIKSPGIPSTAPIVKQAEAKGIPVISEIEFAARYTR
                     AKKICITGSNGKTTTTLLTYHILKNAGLNVGLAGNVGKSFAQQVAESNFDWYVLEISS
                     FQLDNMYDFKAEMAVLTNITPDHLDRYDHDFNKYIESKFRIIRNQTDQDAFICNLDDE
                     VTAEQISRRQIAARCYPFSYDGKKGPEIAFVDRNKLIVTINNDPFIMTLEELALQGRH
                     NIYNSMAATVAARLTEIRKESIKQSLSDFQNVEHRLEYVARIHGIDFINDSKATNVNS
                     TWFALESIDKPAIWIAGGVDKGNDYSKLFDLVRSKVKALICLGTDNSLIIEAFTGKVG
                     AIYETESAEQAVMLAYSIGKTGDVVLLSPACASFDLFENYEDRGEQFKNAVHRL"
     gene            32521..33684
                     /locus_tag="PKI35_04220"
     CDS             32521..33684
                     /locus_tag="PKI35_04220"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HNX43620.1"
                     /translation="MAKLLQNIKGDKVIWGVVIILTVFSILAVYSSTTTLAYRTRQGN
                     TEYYLIKHLSILVFGFILMFLAHKVKYIYYARISQLAIIISVPLLLYTLLFGASINEA
                     SRWTTLPGINLMFQPSDFAKLALIMFVARMLAKRQENIKDFKSAFIPILTPVLLICGL
                     ILPANFSTAALLFVTCIVLMFIGRVNLRYIGAMIGIGGVVLALFLSFLYFSPKSSGRL
                     GTWQNRIEHFVDGDSEENYQVEQAKIAIANGGLLGKLPGNSMQKNFLPNPFNDFIYAI
                     IIEEYGLAGAVIVLLMYLILLYRGIRIVSKSPGNYGAFLAIGVTFSLVFQAMINMAVA
                     VNLVPVTGQPLPLVSMGGTSIWFTSIALGIILSVSKEQESGDMPTNELQNAEA"
     gene            33671..34786
                     /gene="murG"
                     /locus_tag="PKI35_04225"
     CDS             33671..34786
                     /gene="murG"
                     /locus_tag="PKI35_04225"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNX43621.1"
                     /translation="MQKPNHIKVILSGGGTGGHIFPAIAIANAIKARVPDAVIMFVGA
                     KGRMEMEKVPSAGYPIKGVWISGLQRRLTIDNLSFPLKVVYSFVKAGIIIRKFKPDLA
                     IGTGGYASWPTLSAAAALGIPTAIQEQNSFPGITNKKLARKAKRIFVAYEGMEKFFPA
                     AGIRLVGNPIRQDIAFSSVTREEAASFFGLDPAKKTLLVIGGSLGALTINESIRESLP
                     LFAGNNIQVIWQTGKKYAGKAAGAVLKDGKATQITMPFISRMDMAYAAADFVVSRAGA
                     IAISEICAAGKPCILVPSPNVAEDHQTKNAMALASKNAALVIADKDAVEELGKLVVSL
                     SADETKQEELHKNITKMAFRDAAAQIASEALELIKKE"
     gene            34808..36202
                     /gene="murC"
                     /locus_tag="PKI35_04230"
     CDS             34808..36202
                     /gene="murC"
                     /locus_tag="PKI35_04230"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNX43622.1"
                     /translation="MDIRKVESVYFLGIGGIGMSALARYFRSLGKTVAGYDRTRSLLT
                     SALEKEGISVHYEADTSLIEPGVDLAIYTPAVKRDHPDYDFFEKAGIPMFKRAEILGF
                     IADQYKTIAIAGTHGKTTTTTLTAHILTQSQVKCQAFLGGISRNYDSNLLLAPGSSYL
                     VAEADEFDRSFLHLHPHIAVITSMDADHLDIYGNLASMIESFAAYAGNIRNGGMLILK
                     KGLNLPLQAREKVAVLRYSSGEPCDYYPDNIRLEKGLYEFDLHHPSGVIHDLILGIPG
                     WYNIENAVAASACALLNGVTENELRAALNSFRGVERRFDIRLQESGLVYLDDYGHHPE
                     ELKACITSARQMFPGRKITGIFQPHLYTRTRDFADDFARQLSLLDEVILLPVYPAREE
                     PIPGINAEMLLGKITAGKKKLVQKSELPACLDGSSLEVLITMGAGDIDRLVLPIENYL
                     RGFIQNRNNQEARL"
     gene            36199..37002
                     /locus_tag="PKI35_04235"
     CDS             36199..37002
                     /locus_tag="PKI35_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43623.1"
                     /translation="MKMFLKILLIITWIVLAAGAAVLMGFANQSHNQQRCTGITVNVD
                     CNGNEQLVTPEIIKSQLKKEFGKFENKLIGDISIEAINRFLGKNQYIDHSDVHLTVEG
                     QLVADLSQCVPVLRIVTLTGQSFYIDNKGKVMRADPDFPVRVVVATGAIATGTNPIGK
                     NISSWTEGKKNIAPEILNLSKAFQVAAAISNDSIMKALAEQIYVSPEGEIKLLTKAGS
                     HIVLIGDASNLEEKFEKLEAFYRSGLPKIGWNRYKTINLAYKNQVICTK"
     gene            37009..38322
                     /gene="ftsA"
                     /locus_tag="PKI35_04240"
     CDS             37009..38322
                     /gene="ftsA"
                     /locus_tag="PKI35_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNX43624.1"
                     /translation="MESDIIVGLDIGTTKIAVIVGRRNELGKIEILGHGRTESIGVHR
                     GLITNISETVQSIQRAVAEAEQRSGVTIKTVNVGIAGQHIRSIQHKGYIIRRNPEAEI
                     TEEELDNLTNSMYNLAMNPGEKIIDVIPQEYTVDGESGIKEPKGMMGHQLEANFHIIT
                     GQMTAAMNIRKCVERAGYFVENMILEPLASAESVLSEDEKEAGVVLVDIGGGTTDIAI
                     YLDGIIRHTAVVPFGGEIITEDIKTGCAIMKKHAEDLKVKFGSTLARENRNEEVVAIP
                     GLRGRPPKEITLRNLANIIQARMEEIIEYVYSEIKNTGLEKRLIGGIVITGGGALLKH
                     TEQLTAFITGLDTRIGYPNEHLASNSSDEITSPMYATGVGLVMLGMQRNERSRKLTPE
                     EPEAVDQDKKKTKKGVKKTDSQKSWNRGKGFIDWFKDFFDDGSLS"
     gene            38415..39968
                     /gene="ftsZ"
                     /locus_tag="PKI35_04245"
     CDS             38415..39968
                     /gene="ftsZ"
                     /locus_tag="PKI35_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNX43625.1"
                     /translation="MLKFDLPKEQANIIKVIGVGGGGSNAVTHMFKQGIKGVDFLICN
                     TDAQALAQSPVPNKIQLGSNGLGAGSRPEVGREAAIQKADEIRDMFSCNTSMLFITAG
                     MGGGTGTGAAPVIAQVARELGILTVGIVTKPFAFEGRKRDQYADAGIEELKKFVDTIL
                     VICNDKLLDLQGDLKLSQAFGKADDVLTIAARGIAEIITVTGRINVDFEDVKTVMLKS
                     GKAIMGAGIAEGPNRAIDAVQMALNSPLLDDTDIKGADNILLYITSGKNELSLEELNE
                     ITNFITDQAGEDANLIWGDSIDEALDDEISITLIATGFDKRDRIVHNIDGSEQYKSVM
                     NLPVNKPAAIQEVTLVPKDKVPAEPVETPQRIAVATPELTVSLFENPAMAGKPEDIIP
                     ENAEQPEPLFKVAPPVIKEPELVITNTCQVNNPVKKEEAQKNQPELENIQYDRISKLK
                     EMSMHLKRPGTIEELERQPAYIRRNVELTDMSSSQGSIQSRYTIGMNDNQQPNSLKEN
                     NSFLHDNVD"
     gene            39987..40445
                     /locus_tag="PKI35_04250"
     CDS             39987..40445
                     /locus_tag="PKI35_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNX43626.1"
                     /translation="MELEKLINDDIKQTMLARDSRKLDALRAIKAALLLEKTGKDLKW
                     DGEIPEEVELKLLQKLVKMRRESAELYKSQNRPDLAEEELFQAGIIEKYLPAQMDEAE
                     IETVIKAIITETGAQNIKDMGRVMGAATKKLAGKADNKAISEIIKRLLGS"
     gene            complement(40718..41551)
                     /gene="tsf"
                     /locus_tag="PKI35_04255"
     CDS             complement(40718..41551)
                     /gene="tsf"
                     /locus_tag="PKI35_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNX43627.1"
                     /translation="MAITAADVNKLRQMTGAGMMDCKNALVESEGDFDKAIDILRKKG
                     QKLALKRADRDANQGIVIAKVTDDKTFGAIIMLNCETDFVAKNEEFIDFANKVIDLAI
                     QAKPADLDALKALTINGRTVAEGVTDLIGKTGEKTELAHYNCIEAPVVVAYNHFGNRL
                     ATILGFNKAGVPGFDETGKHVAMQVAAMSPIAVDKDDVAKDVIDREMEIGKEQARAEG
                     KPEEMLEKIALGKLNKFYKESTLLNQEFINDSKLTVRDYLQKADKELTVKKFNRLQLG
                     A"
     gene            complement(41639..42556)
                     /gene="rpsB"
                     /locus_tag="PKI35_04260"
     CDS             complement(41639..42556)
                     /gene="rpsB"
                     /locus_tag="PKI35_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNX43628.1"
                     /translation="MARTNFDELLDAGAHFGHLRRKWNPNMAPYIFMERNGIHIIDLY
                     KTIAKIDEAAGALKQIAKSGKKVLFVATKKQAKELVSELVKPVNMPYVTERWPGGMLT
                     NFATIRKAVKKMSSIDKLMASPSFSNISKRERLQITRERAKLEKNLGSIAELNKLPSA
                     VFVVDIMKEHIAIAEARKLNIPTFAIVDTNSDPNLVDFPIPANDDASKSIALVVKKMI
                     EAIEEGLAERKLDRDKRAEEEHEEYDEHGERIIRDVDEEEEETDEESREAAHKKRVAG
                     EEGSSEERAKRPRKVVARKPGGSAPRKRP"
     gene            complement(42673..43059)
                     /gene="rpsI"
                     /locus_tag="PKI35_04265"
     CDS             complement(42673..43059)
                     /gene="rpsI"
                     /locus_tag="PKI35_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNX43629.1"
                     /translation="MENTKTVGRRKCAVARVYLKEGNGTITVNGRDYKEYFPTAILQN
                     TVEQPFVVTNTVAKYDVNVTMDGGGVKGQAEALRHGISRSLCEINPEFRPALKKEGFL
                     MRDPRMVERKKYGQKKARKRFQFSKR"
     gene            complement(43065..43520)
                     /gene="rplM"
                     /locus_tag="PKI35_04270"
     CDS             complement(43065..43520)
                     /gene="rplM"
                     /locus_tag="PKI35_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNX43630.1"
                     /translation="MNTLSYKTQTPKGGEIKKEWVLVDAENEVLGRLATKVAAILRGK
                     YKTNYTPHLDCGDNVIIINADKVRLTGAKMTEKEYIHYSGYPGGQKVGTAKEMLRKKP
                     AFLVEEAVRGMLPKTRLGSELFRNLRVFAGPEHDHQAQQPKLINIHEIK"
BASE COUNT        11937 a         9842 c        10482 g        11471 t
ORIGIN      
        1 taacatcact gtctgcaatt gccagcggga aatgaaagcc gcccggataa tacaattttc
       61 tgagataaat taccacgatg cattcaaaaa gccccatagc tatcgaaagc aaggtgatcc
      121 agatacagag tgcaggtttt ttcatggtgt ttttatccgg tataaagata ttccgtgtat
      181 aatagtgtcg ctgcaaacag gaagatatgc atcagtttat aaaaataacg tgaaaaggca
      241 acataattat cagattaaca aatatataaa aatttaaata tttaaatcat ttaaccggag
      301 aatttcataa ttttgcaccc tttattttaa aaagaatgaa tagtgaaatt gaaatgacgg
      361 aatgcctgat aattggctct ggtccggctg gttatactgc cgcaatttat gcctcgcgtg
      421 ccgatcttaa accggttttg tacaccggcc ttcaaatggg cggacaactt actaccacta
      481 ccgaagttga taactttccg ggttaccctg agggtgcgaa aggcccggat atgatggaag
      541 atatgcgccg tcaggccgaa cgtttcggcg ccgatatacg ctttggcatc atcagccagg
      601 ttgatttcag ccgccgtcct ttcagtgtga ccactgatga cgggaagacg gtagaggccg
      661 aaacagtcat cattgccaca ggcgcttctg caaattggct gggactggag tcagaaacgc
      721 gatataatgg gcacggggtt tcggcttgtg ctacctgcga cggattcttt taccgcggcc
      781 tggatattgc agtggtcggc ggtggcgata ctgcatgcga agaggcttct tatctggcca
      841 agctgggaag aaaggtgtac atgatagtac gccgtgatga actcagggct tccaaagcca
      901 tgcaacaccg tgtaatgacg gcgcccaata ttgaaatcct ctggaattca tcaacaaagg
      961 aagtgcttgg tgacggcaaa gtggtgacag gcgccctgat ttccaataat aaaacagggg
     1021 aactgcggca aattgagatt cagggatttt ttgttgccat cggacataaa ccgaatacgg
     1081 atgtgttcag gggacagatt gaactcgatg agatgggcta tattaaaacc atccccggga
     1141 gcacgcgtac caatgtagaa ggcgtttttg ctgccggcga tgtacaggat catgttttca
     1201 ggcaggctat tactgccgcc ggaactggtt gcatggccgc tattgaggcc gagcgctggt
     1261 tgctcctcaa caaataataa tttttcggaa tccggttatg gctgcctctc ccggcagcct
     1321 tttttttacg ggacccggga gcctatgcct ttttatacgg aatagaaaac cagaaagtag
     1381 agccttttcc cagctcactt tcagcccaca tttcacctcc catcagttct acaaattttt
     1441 gagctaccgt aaggcccagg ccgagcccgc catactgccg cgtctgggtt tcgtcaacct
     1501 gcctgaattg tttgaatatg atatccagtt tgtctttcga aaatccgata ccttcatctt
     1561 tgacgaaaaa atcaattttc gaatccctgc cgaaccgata ccctacatct acatagcctt
     1621 cttccgaata ttttaaagcg ttctcaataa ggttgttaag ggtgcgacgc aacatgtact
     1681 catcgctctg gatggcaaaa tcattgtctt tatttgcttt ctcaatcctg aactgtacca
     1741 tttcctttcc gagggtggat ttttccaggt taaaccagga ttgaagactg ccaagcatat
     1801 cgtttatttt taccggtttt tcttctaccc gggtgatttg cgattcgacc tgggcaatat
     1861 caaaaatatc attgatgagt tcaagcaacg aatcaacgtt acggttgata atggaggcat
     1921 actgaacttt atcggtatcg ggaagggtgt ggtttgcaat caaaccgcaa aaccccataa
     1981 tggcattaag cggtgtccgg atttcatgcg acatgttaca caggaatgcc gacttcaacc
     2041 tgtcggattc tccggcttgc tgccggacaa tatgtatctc ttctttcagc ctctccatct
     2101 catcaaccag aagactgttc tcccggagaa tgcctttgag tttgcgacgt tgcctgaagt
     2161 acataaaaat caggcatgca gccaacacca ttgcagccgc caggattaac ctggccagaa
     2221 gccatggttc attgccggtc tgcgatgtca gaaacaaatt gtgagatgaa accagaaaca
     2281 tattttgtga ttgttaagat agcggtagag caacacgttt gtttattcca gcccgattgt
     2341 aatatggctt aatcaataac aaacctacat aattttcagg agctttattg attataaact
     2401 tcagcgttga aaatttcaac ctgttcagca cgggcttcaa tgggaagcat cagccacaat
     2461 tgaacattat ggagttatac gatgaatgcc aacggggtat tccgaaagga ttatctcctt
     2521 gagtttcggg tagtctcccg gattttcaac aaaatcaatc aggtttgtat cgataatcac
     2581 aatgcgcatt tccttttgct gcctgatgta atcgagatac ccgtcctgaa ttttttgcag
     2641 gtattcggga gcaatcttct gttcatagga gcgtccgcgt ttcctgatgt tcgtttgtaa
     2701 cctgtcgacc gaggaataca aatagatgag caattccggt ttaggcaaaa ccgaatcaat
     2761 aatgctgaat agctttgaat acagcatata ctcatcgttg ttaagcgttt tccgggcaaa
     2821 gatcagcgac ttgtggatga agtaatcggc aacggtgaag gggctgaaca ggtcatggct
     2881 ccccacacct gttttcaatt gctgaaaccg gtcggcaagg aaagataatt cgagcggaaa
     2941 agcatagcgt tcctggtcct cgtaaaattt tggcaggaat gaattatcct cgaattgttc
     3001 cagaatgagc ctggcgcctg tttcggcaga aagccgtgtc gccagcgaag ttttgccggc
     3061 tccgatgtta ccttcaatgg cgatatagtt ataatggaat ttatccacaa cagtcttttt
     3121 agttatcatt acacaaggtt ccgggaaatt tttcccttta aatttctgcg gccttcttcc
     3181 aaacccttac cgtgcattca tcatcaacgc tttcgaggag ggaagatatt ttccttttca
     3241 ataccggatg tattctgccg ggtgaaatct ccgccagcgg aaccagcaca aatttgcgct
     3301 gatgcaaccg gggatgggga atcagcaggc cttcacggga aataatctgc cgtccataca
     3361 ggagtatatc aatgtcgatg attcttgact cataagctgg actgttcctg ctgcgaccca
     3421 tctctttttc tataccatga attgttgcgt tcagcatatc gggagtaagg gaagttcgca
     3481 atatcagtac ctggttcaga aaatttaatt ctgcgttaaa cccccagggc tcggtttcat
     3541 aaattgaaga acagcgtatt accttcccgg cctggttgtt taatgctgtg agggcctgtg
     3601 aaagcgcagc ctcacggtct cctaagttac tgccggttga aagatatacg attgccatga
     3661 ttatttcgcc ccaaatttac aataaggcga cagtataaag ctttccatat ggggatgcct
     3721 ttaaattttt tttcaacagg aatacttttt ccggcccttt catgaaattt ggcaagaggc
     3781 ctttctcttt gtaactttgt ctgaattcct tcgtcataat caataaatcc taaaaaagcg
     3841 cacgatatga aacaattttt aaaaatgaca tttgcttcca tgctgggatt catcctggcc
     3901 ggtttgctca tctttgtcat cttcctcggt attgtggctt ccgccctttc ttttaccaag
     3961 aagcaggtcg ttgaagttcc cgctaataca gtattgcaaa taacgctcga tcagccggtt
     4021 aatgaccgcg cctcggaaga tccgatgtca tacctgaatt ttagggatat gaccgctaac
     4081 aaacccatgg gcctgaccga gatactcgaa tccatcaaga gagcttctgc cgataaaaaa
     4141 gtgagcggga tatttctcga tctggccgaa atacctgcaa gcatcgccac tattgaggaa
     4201 atccacaatt cattgctgga ttttaaaaaa tcgggaaaat ttataatcag ctattctgaa
     4261 ggatacaacc agaaagccta ctatatagcg tcggtttcgg ataaaattta cctgaatcct
     4321 gccggaagta tggagtttaa aggcctttca ggccaggtga tgttttttaa aggattgctt
     4381 gaaaagctcg atatcgaggc acaggtaatc cgtcacggga aatttaaaag cgccgttgag
     4441 cctttcatcc ttgataaaat gagcgagcca agcaaggtgc aaacgctttc ctacgtttcg
     4501 gccatctggg atcatatgct gcagggcatt tccgatgcca ggaaattgcc cgctgccgat
     4561 cttacggcaa tagccgacaa ccataaaatc cagacgccgg aagatgcagt cgcgaataaa
     4621 cttgttgata aactgatgta taaagaccag ttgctcgacg aactgaaggc gcgggtctcg
     4681 gccaaatcag tgaaagatat ccatgtcatg aaattatcaa aatatatgga tgccccgaaa
     4741 cctgaaaagc gtgaagcgtt ggcaaaggac aggattgctg ttatatatgc gcagggaagt
     4801 atcgtgagcg ggaatggcga tgaccagaac ataggatcgg aaaggatatc aaaagccata
     4861 cgcaaagcga ggatggacga gaaagtaaaa gccattgtat tacgcgtaaa ctcgccgggt
     4921 ggaagtgcgc tggcgagcga tgtaatttgg cgggaagtta cgctggccag gaaagaaaaa
     4981 cctgttgtag tttcaatggg cgacctggct gcatcgggcg gatattatat ttcctgcgct
     5041 gcaaacaaga tatttgccag tcccaatacg ctgacaggtt caataggcgt attcggaatc
     5101 gtgcccaaca tgcagaaatt ctttgataaa aacctgggta ttaccttcga cgaagtaaaa
     5161 accaatcctt atgctgatta tattccgaca acacgcccga tgactgaagc tgagaaagga
     5221 tttttgacca acgatatcga gcatatttat gctactttcc tgagccatgt cgcagagggg
     5281 cgtcatatgt cggttgccgc aatcgacagc attggccagg gacgtgtctg gagcggaaca
     5341 gatgcaaagc gtcttggcct gatcgatgaa tttggcggtt tgaaggatgc tgttgctgaa
     5401 gctgcaaaaa tggcgaaaat tgaaaaatac cgtattatca gcctcccgga gcaaaaagat
     5461 gcattcacgc aaatcattga agcattcagc ggcgatgaaa cttcggtgct gaaaaaggaa
     5521 cttggcgccg cttatccata cctgaagtac ctgcagaata tgagccggat ggaaggtgtg
     5581 caggctatgc tgccttacga tatcaggatt gagtaagaga caggatatct gataaaaatg
     5641 agggcgtccc ttccgggagc gccctcattt tttatatacg gttttctagc atccgaggta
     5701 ataaaaaaac cggagaggat ccccggtttt taaacaatga agcacgagtc atacaagtta
     5761 tttagtgaag tcagtttcgg ataatcgctt gtaggtatta tacctccatt ttgcattctt
     5821 ttccgctgtt gcaaaaagct ctgcagcttc ggtggggaag gttttcatca gcgaggtata
     5881 ccttacttcg gacttcagga aatcctggaa ttttgtccaa tcgggttctt tggaatcgag
     5941 ttggaacggg ttctgcccct gtgattcaag ttccggattg aagcggtaga ggtgccagta
     6001 gccggcctgt actgcctttt cctgctgatc ctgcgctttc tccatgccag agtgcaatcc
     6061 ctggttgata catgttgaat aggcgatgac cagtgaaggt ccgggataag cttcggcttc
     6121 gcggatggct ttcaggtact gactctggct ggcgcccata gctacttgtg ctacataaac
     6181 gtatccatag ctcatggcca tcatgccaag gtctttcttg cgaaccctct tgcccgaagc
     6241 ggcaaatttg gcaacagctc ctgccggagt tgattttgaa gcctgtccgc cggtattact
     6301 gtaaacttca gtatccagaa ccaacacgtt gatatcttcg ccggaagcaa gaacatggtc
     6361 gagtccgcca taaccaatat cgtatgccca accgtcgcct ccaaaggccc atacactctt
     6421 cttgacaaag taatgcttca gggataccaa ttctttgcaa aggccgtcgc tttctttttc
     6481 gagaagaggc agtaccttta acgtagcggc ctgacttgag gctgccttgt ccttgccggc
     6541 aatccattcc ttacaggctt ctttcagttc ttcagaataa ttcccttcaa gaatactttc
     6601 catgcgtccg gcaatccggg cccttaattt tcctacgcca agggccatgc ccagtccata
     6661 ttcggcattg tcttcaaaca gtgaatttgc ccaggcgggg ccacagcctg atgctttatt
     6721 gacggtatac ggtgtgctgg gggcggaacc tccgtaaatt gacgagcaac cggttgcatt
     6781 ggcaaccatc atgcgttcgc cgaacaactg ggtgatgagc ttgatataag gtgtttcacc
     6841 gcaacctccg caggcgccag agaactcgaa cagcggctgt gcaaactggc tgtttttaac
     6901 attctggtct ttcggggcta gagtatcttt ataagtaact ttttttgtca tgtaatccca
     6961 gttgtctgct tggtgcaact gggtatccag atgccgcata accagggctt tggttttagc
     7021 cggacaaaca tcggcgcagt ttccgcaacc ggtgcaatcg agcgggctta cctgcatcct
     7081 gaactgcaat ccgtcgaatg tttttggaat agccttcagc gttttcgttc ctgccgacag
     7141 ccccttcatt tcatcttcgt tcagtaagaa cggacgaata gcggcgtgag ggcatacaaa
     7201 ggaacattgg ttgcactgaa tgcagttttc agcctgccat tcgggaacat ttacggcaac
     7261 gccacgtttt tcgtaagcgg tggtaccggc agggaaagtt ccgtcttcac ggcctttgaa
     7321 tgcgctgaca ggtaacaggt cgccttgcat ggcattgata ggctcaacaa ctttacggat
     7381 gaattcggga acgtcgtttt ggactgtttc ttcaatttta actatgattg atttccattc
     7441 agcaggtatt tcaacccttg taacggcagc gcccctgttg atggcttcaa tattcatttt
     7501 gacaacatcc tcacccttgc ggccaaatga ttttttcacc gccttcttca tttcttcaag
     7561 agctgtttcg taagggatta ctcctgagat cttgaagaaa gcagcctgcg tgatggtatt
     7621 ggtacggttg cccagtccaa tttcttcggc tatggctgtc gcgttaatga tgtaaaaatt
     7681 gatctcgttt tcggcgagga attttttcat actgtcggga aggcgtttgc gggtctcttc
     7741 ggcatcccag atgctgttca gcaggaaggt gcctcccttt ttcaatcctt tcagcaggtc
     7801 atacttattg atataggcag gaacatggca ggctataaaa tcaggagtgt taacaagata
     7861 cggcgagcgg atgggtttgt cgccaaacct gaggtgggat gtagttacac cgcctgattt
     7921 tttcgaatca taagcgaaat atgcctgaca gtatttatcc gtggtttctc cgatgatttt
     7981 gatggagttt ttattggctc ctaccgtgcc gtcagatccg atgccaaaga attttgcctg
     8041 gaatgtcccg gcatcagcaa ggtcgatttc cggaagaacc ggcaatgacg tgaaggtgac
     8101 atcatcaacg atgcctaccg taaaacggtt tttcggctca ttcagtttca ggttattgaa
     8161 taccgagatg atcatggccg gagttgtatc ttttgaacta aggccgtaac gaccgccaat
     8221 aatcaggggt ttatggtcgc gttcatagaa agcttcacga acatcgagat ataatggttc
     8281 gccgttagcg cctggctctt ttgtgcggtc gagaacgcag atacgtttca cgctttcggg
     8341 catggccttg aagaaatatt ttgtcgagaa ggggcggtac aggtgtacgg tgacaaggcc
     8401 gagtttttcg cctttggcgt tgagatggtc aatcacttct ttgatggttt cggtgattga
     8461 acccatggca acgatcacat tttctgcatc cggggcgccg taataagtaa atggatgata
     8521 ttcgcggccg gttacttttt tcatctgttg catatactct tcaaccaggt cggggagagc
     8581 cagatagaat ggattagcgg cctcgcggct ttggaaatag atgtccggat tctgcgccgt
     8641 gccgcgtgtc ttcggaagct ccggattcag ggcgcggttg cggaatgctt tcagggcatc
     8701 gtaatcaacc agacctgcca gcgattcagc ggtgggggct tctactttct ggatttcatg
     8761 tgaagtacgg aaaccatcaa agaaatgcag gaatggaacc cgtgatttaa tggcagcgag
     8821 atgtgcaaca gcagcaaggt ccatgatttc ctgaacgctg cctgtcgaca acatggcaaa
     8881 gccggtctgg cgggtggcat agatgtcgct gtgatcgccg aatattgaaa gagcctgggc
     8941 agccagacta cgggccgaaa catggaaaac acccggtaat aattcaccgg ctattttata
     9001 catgttggga atcatgagca ataagccctg tgaagctgta taagttgtgg taagtgcacc
     9061 cgactgtaat gaaccgtgga cagctccggc tgctccgcct tcactctgca tttcaattac
     9121 gcgaacagtc tctccaaaaa tgttcttttt gccaaaagca gcccattcat caacatattc
     9181 agccatggtc gatgatggcg tgataggata aattgaagct acttcgctga acatataagc
     9241 aatatgcgcg gctgcatagt tgccgtcgca tgtcacgaaa ttcttctttt cggtcatatg
     9301 atgtataatt tattaagttg acttcagaaa tttttttaaa aagtttcaga aaagtgctgc
     9361 aaaattaccc attattataa agcaaatgac ggttttagat atggatttca tttttaatgg
     9421 ttttatttaa aatgattatt aataaattaa agtgatttct ggcgtcgtgc agtaaaatat
     9481 tcattcacag tggattaaac tttttaaaat aaatcatggc accggaatgg tgcgtatatg
     9541 tctcctttgt tttaagggtt gaagggaaca taataaaagc cggtatgggg gaaataaccg
     9601 gctttggtga gggggaattt ggggggggga tgattattct ttagcgattt tcgaaagcaa
     9661 tttgattgct tcctgctgat ggaaattatt atcgtttgca gctatcagtt tgagctggtt
     9721 tattgctttg tcggtttggt ctgatttcag gtagcaaagg ctaaggtacc attcagcatg
     9781 ttctacataa agattgcgat tgtcgcggat gatcttttca aatgcggaaa ttgcttttgt
     9841 gtagttcttg gtttcgatgg atgaaatacc gtaatagaac caaacggcca tgtttgattc
     9901 atctttttcg aggattcttc cgaattgcca ggcggccatg ttataatcct tttcctggaa
     9961 tttcatgatg gcttcaacaa tattggcatc gcccgaacgg gtaacgtcga ggattttttc
    10021 ggaggaataa tactgggcaa acaaggcatc gttagatgtg cggttaggca aggaaaacag
    10081 cagggtagcc ccgatagcca gcaccgcaac tacagatgcg gctgcaaaat accagcgacg
    10141 ggaatgcata tggatcacgc ggttatgtga gtaggatgct ttctgctgtt catacaaata
    10201 gttcacctta cggcgcacat caataatatc atcccttttg agggcattat caatctgttg
    10261 tgaaaaccgg aattcggctg ccatttcctt attagccgcc aattctttgg caaaattttc
    10321 ctcttctaca ggagtcatct ccttgttgag aaattgctca atacttgctg aatgtcgaaa
    10381 tttaatcata gctaatcatt aaaaaatttt tggcatctgg gatcgttggc tattcttttt
    10441 ttcaattctt ccttacacat gtatttacgg gtttttgcat acttttcggt cttgaaaccc
    10501 ataatgtttc cgatttcacg taatgagaca tccttcataa acaggcggag aattttctgg
    10561 caatcttcgt tcagcgacag aaaatgaagt tgaacaagcc tgtgcaattc gatttcttca
    10621 ttactgattt catgcatgat atcttccggt aagtcgatat actcctctac atcgcttatc
    10681 ttttccatga cagtttttcg tttgttcaac ttctgcagcc atagattccg gcaaactgag
    10741 tacatgtatg ttttgaaaga actggtaagc gttaactggt tatcggttat ttttttgtaa
    10801 atgatgatca atccatcctg aaagatatct tcagcgtctt catcagcacc tgaattctcg
    10861 tttaccaggt actttatcaa tggaaagtat tcccggtaaa cgaaagtgat aatgtagtcg
    10921 ctcctcagcc taagcccttc aactatcgct tcgtcagaat aatgaatcat ttacttcgta
    10981 tattatccgg attttgtaaa aaggtaatac catttagcga ttttttttcg atttttttca
    11041 ttcgttcata attagcagta aaacaaatcg ttaaccggtt tattaacaaa ataatcgcta
    11101 atggttaata acctaaagca aaattggtaa taatttcctg aaaaaatgaa aaaaaaagta
    11161 taatttataa aaatttctca ttcaaaatag ggctaaaacc gtattacctt tttaaagtta
    11221 gtggataaat acctaacaaa acaagatgtt gttggcattt gaaattggta taaaagtcag
    11281 ataatcagtt gttaagagtt ggttggcgtc gtgtttgaaa aaaaaatcag ggtgaacaat
    11341 aattgatcta acttaaacct ggcaaattaa aaaccaatga actgccacga attaacaact
    11401 tcattcttct aatttaattg tacatctatc atgaaaaaga cttaccgaaa ttttctggta
    11461 ctcgttttta cccttcttct gggaacagct tcagcttcaa ctgtttccgg tgtattactg
    11521 taccttgata atccgcagaa tcctatcccg aataccgttg tgaaactcac aaacctcagc
    11581 acccagtcgg ttatgactgt tgtgacggat gataacggta actatgaatt tactgatgta
    11641 gtgcctggta actacaggtt atcggcaaca acctctctcg caagcggcgg cgttgacctg
    11701 ctggatgccc tggaagtgct gaaatatctc atcgggcaaa caagctttac acagattcag
    11761 cttctggctg ccgatgtaaa cggaagcggg tccgttaaat tgagcgactt tgttctcatc
    11821 atgaatcatt tgctgcatgg tactcccttc cctgtcggca attgggtatt cgaaaccttt
    11881 aattttaccg tttcgggaac caaggaagaa ccgggcgggc tgagtggttc atcatccgga
    11941 gacctgaccg gggtatttgt acccggtgga cgtaatttac ctgcattccc ggttgaacac
    12001 aatgcgaaaa ttgccgctac tgctgaaaat gaaattgaaa tccccctgat tacctatgat
    12061 gcgctcgaac tgaccggcgc gggacttata ttcaactacc ctgggaacct gatatcaatc
    12121 gaatcggttg aattccccgg caatgatttt caatatgcca tcagtggtga tcaactcaaa
    12181 ctatattggt acggtgaaag cgatgctccg gtttcctttt cagccggatc ggctttagca
    12241 accctgcatt gccgtactac agcggccttt gctcctggta ctgaagcaga cttttcgctg
    12301 gatggttcaa cctgtctggc aggcggcaat tataccaaga tcaccggtgc aaaactgggc
    12361 atacctacaa ttacctatgc cgcaccaagc ctgaggatca gcaactatcc aaacccgttt
    12421 gccgaatcta ccatgattac ctattatctg cccgaagatg gtaatgtcac tatcagaatc
    12481 tactcatata ctggacaact ggttttaacc atgcccctgt cggacaggaa agaaggctac
    12541 aaccaggtga acctggatgc cgccaactgg cttcccggga attatatctg ccggattcag
    12601 agcaacggca gccggaatta caacgaatca cgcatcctga tcaaaaccaa ctagatgtat
    12661 taatatagat agatagttca gggaggtgta aaaacctccc tgttttttta tccggcttgc
    12721 tattgagtaa gattgtgttt atttaccttt gcagatatta ctaaaatcgt tgccaataat
    12781 atctgtccct gttatgcaac tgccgctgct gttaaggaaa tgcaaaaatg caagcctcat
    12841 aaccataacc ggtgttgcct gtcatgtgct gatagttatt ttactacttt ccggttgctc
    12901 aggtaatcat aacgataagc ggcctgttaa tcagggaggc gatcttgaga caggtgaagc
    12961 ccttatgcag caatcgcttt tcgatagcgc tctggtttat tataaccggg ttgcccgcaa
    13021 cggactaaaa accggctcct gggacatctg gtataagggt actgccggct tgattgattg
    13081 ttacagggca aaaggcaatt ttgaagaagc tttcaggatc gctaaccagg cgcaacaaat
    13141 tgcctccggc agaggtgata catctgatgc catatatgca ggcatacttc acaaaaaagc
    13201 agtaatcctt acggataagg gagattatac cacttcatcc tctgtgctca gtgaatctat
    13261 acttatccgt tcccggggta aggaaaatgc agatacagcc atggccttat cttataatat
    13321 cctgggaaat aataatttgt ttcagggtga atatgataaa gcacttgaaa actatctgaa
    13381 ggcgatcaca ctttttaaag acaggaaaaa ttctgctgat ctggctacca tcaaccagaa
    13441 tgcaggaatc gtatatgcgc tgaaagggga ttatgacaat gctaagaatt tttttcatga
    13501 ggccctgacc atcaaccagg cagtgatccc ggccaacgat ccaaaactgg ccttgatgta
    13561 cctgaattat ggcaggtatt attctctcat tgaaaatgac cgcgaagcgc tggaaatgta
    13621 caataaggct gaaagcatct tcaggaaatc aggcgacaaa agcaccaaaa acctggcccc
    13681 gctttaccac aatattggaa atatttatgc caattatgct gattttgata aggcgttatt
    13741 gtatttcaac aaagcccttt ctctttatca gtcgcatccc tcggaagggg atcagaacat
    13801 ttcagcaatc ttattaaaca taggctatat attcgaaaag gaaggcaatt accagcaggc
    13861 cctgaaatac tatctgcaat cggttgagaa aggcaagaac acccagaaca aagtgactat
    13921 ataccgtaac ctggccaata tctactatat tatcaaacag ccggaaaatg cttttctgta
    13981 ttatgatctt gccctggacg caagcctctc tggatttggg cctgatcatc cggaaacagc
    14041 gctgacctat ttgaaattcg gtgagtttct ttcattgaca ggtcgcgatc atgaagccat
    14101 cactgacctt aacaaaggat tacaggtata ccagaaactt tttggcagca aaaatcccca
    14161 ggtagcagca gcatataagt atctcggcaa tcattataca cgaaagaaca atttcgaaaa
    14221 ggctctcaac tgtttccagc aatcccttat tgcaggtttc gcaaacttca acaagacgga
    14281 ggcagcgtac aatccggcta tctctaaaag tgaagttaac tattttatgc tgaattcgct
    14341 gagcggcaaa gcatttgcac tgagaagcct ctacctgaag aatcacagcc gcaccgatta
    14401 tctcgtttcg agtgcagatg cctatgatgt ttccatctgc acgatcgaga tgttgcgttc
    14461 ttcctaccag gacgaagaca gtaaactcct gatggctggc aacgaaaaat ggatattcca
    14521 gaatgcaatc caggttaatt cagagcttta caaccgttat agggataaga aataccttat
    14581 aaaggccctg gaactctctg aaaaaagcaa atcggcagta ttactctcga atatccgcaa
    14641 tctggaagct aaagaagtcg gtaaaattcc cgcaaaactt cggattcagg aaaacaagct
    14701 gaaagaggag ataggccttt atgagaagct tgtttatgac gaaaaatcaa aggctaacgc
    14761 cgatgcgcag aaaataaact tctggaacaa caaaattttt gagctgaata agtggtacga
    14821 cagccttatt gtcatatttg aaaaagagta tcccgcttat tataaaatta agtatgacaa
    14881 ttcggttctc gggctctccg aaattcagcg caaactgaaa gataaccagg ctgttattga
    14941 atataccatg gatgatgaat cctatctgtt tgcttttgtg attacgcgaa gatactgcac
    15001 cctgaaacac atccccctag attctctttt cttcagcgac atcaataccc ttaccggcca
    15061 gatggcggga aaatcattca acaattttag ccaggatgat tttacaagtt ttgtctccgc
    15121 ttcacgaaga ttattcgaaa agcttattcc tgaagagata tcggagcatt tgccgcccga
    15181 tttaatcata attccggatg gggaattagg ttatttatcg ttcgatgtcc tattgaccga
    15241 aaagccttcg gaccaggctt ctggttaccg gaaccttcct tacctgataa agaaatgcgc
    15301 cattaattat gccccatcag ccaccatgct gtttggtgag cttagtaata aggggaaacc
    15361 cggacatgat gttcttgctt ttgcgcccag ttacgataat ctcaaaggaa taccgatcaa
    15421 cctgatgatg aaccggcagt cggctcagaa ttacctcctc ccgattcccg gcgcccagat
    15481 tgaagttgat aatctgaaga aaatttttaa ctgcaaggtt tatcagaaca acaaagccac
    15541 aaaagataat ttcaggaagt ttgccggcga ttacagtatt cttcacctgg ccatgcacac
    15601 gctgatcgat gataagaacc ctttatattc aaaactcgtt ttttatcagg ataaaaagcc
    15661 tgacgataat ggccttctca atgcttacga acttttcaac atgcagttga aggcccaatt
    15721 ggcggtattg agcgcctgta atacaggcag gggcaaactg gttagcgggg aaggcattct
    15781 tagtctttcg cgtggatttt tctatgcagg cgttccgagt attattatga ccctctgggc
    15841 tgtagaagac cgttccgggg ctgatttgat gacctctttt tatcattatt tatcggaagg
    15901 gaaagccaaa aatgaagcgt tacggttagc taaactcgat tacctgaaat catcggacca
    15961 gatccgttcc catccgcatt tttgggctgc ttacctgagt atcggcaata ccgacccggt
    16021 tcaggatatt ggtaaacccg tttcatcggc taagtacata ttgggttcag cattcatact
    16081 ggcagtggtt gtcgcaacct tttgggccta taggcgccgc cgcaggtcca gcctatagtc
    16141 aggcagcctt tctgatggca attttcctac ttttgtaaaa aatctgatcg tgatatatcc
    16201 cggaaatttc gaggaaaaaa tcggctttga tgttatacgt ggtttgctaa aggataaatg
    16261 cctcgggcct gccggaaaac ggctgattga cgccatcaca tttgcaacca ggtttcagga
    16321 tattgacccc gaactcttag ttacgggaga gttccgcgaa cttcttctaa cggttccggg
    16381 ctttcctatg tccgattacc tcgatctgga tgaagaatta cagaggatcc ggacggcagg
    16441 aacatttctt attcctgaga acctggttca gctaaggcat tctttgtctg ttgtttctga
    16501 tgttctgaat tttatccgct cactcgacag ctactcattt ccggcgacga tcaggcttgc
    16561 ggaagacgtt tttgtggatg gcaggattat atctgagatt aaccggatat tgaatgagca
    16621 aggtgaaatt cgcgataatg cttctgaaaa actgttttct gtccggaaaa gtatagtaag
    16681 cctgcgatca caaattgacc gtcgtattgc ccaatcgctc acacaggcta aaacgcaaag
    16741 ctggatcagt gaggaagccc agattaccat ccgcaacgga cggcttgtga ttcctgttcc
    16801 ggctgcaaat aagcgccgga tcaaaggctt tattcatgac cagtcggcta ccgggcagac
    16861 cgtttacata gaaccagccg atgtatttga actgaataat gatatcaggc aactggagtt
    16921 gtcggagcag caggaaattg tccgcatcct gatccagttt actgattttt tgcgccccgg
    16981 cctcgataat atttccatag ccttcgattt tctgggacgt gtggattttc taagggcaaa
    17041 ggctgttttt gctcttgaaa ccggcggtac aaaacctttc ttccggaacg accggaaaat
    17101 tgattggatt gaagcgcggc atcctttact attcttatct cacaaacggc tgaacaaacc
    17161 ggtaatcccg ctggatatac gtcttgatga agaaaacagg atcctgatta tctcagggcc
    17221 gaatgctggc ggcaagtctg tatgcctgaa aaccgtcggg ctgttgcagt atatgcttca
    17281 gtgcggctta ctggttccta tgaaggaaac cagcgaaaca ggtgttttta ccgatttatt
    17341 cattgatatc ggcgaccagc agtctattga taatgacctg agtacctata gcagccacct
    17401 gataaatatc aaaaacctgc tgagctttgc cggaaaaagc agcctgtttt taattgatga
    17461 attcggggcg ggtaccgaac ctcagtcggg cggggccatt gccgaaacgg tgatcgaatc
    17521 gctgaatcag aaaatggcgt ttggcgttgt cacgacccat tatgcaaacc tgaaattgct
    17581 ggcttccaaa ggcagcggaa ttgtgaacgg ggcgatgctt ttcgatacac gaaaaatgaa
    17641 acccttgttc aagctcagca taggcaaacc gggaagttct tttgcctttg aaatagcttc
    17701 caatattgat tttccggcag agatactttc aaaagccatc gaaaaagcag ggacttcgaa
    17761 actcaattac gacaaactaa tacagggact tgaaaacgag agggccgaga tttcgcttaa
    17821 ggaaaaacag ttgcagcatg cagatgacct gcttgcttcc ctgatttcga agtatgaaac
    17881 tttaaaaagc gatctggaaa agaaaaggaa gtcgatcctt gaagatgctc aggctgaagc
    17941 gcggcacatg attgcggatt ccaataccct aatcgaaaag accattcgtg aaatccggca
    18001 atcgcaggcg gaaaaatcaa aaacacggca actccgtcag gaaattaccg aaatggcagc
    18061 taaactggaa acggatgctt cccccgatcg ggaatcgata ccggcgactg ctccggaaag
    18121 ccagcctgaa cctgtcgtca aagcttttcc ggaaatcggg gaccccgtga aaattgaagg
    18181 acaatcggag atcggtgaga tcgaagaaat gaccagcgct gatgccctcg ttgcctttcg
    18241 gactgttaaa ttgcgtgttc cgttaaaccg gctcatcccg tttgaaaaaa cgggggataa
    18301 accaaaggga aagagcgctt atgattatcg gtatcataac atagttaacg agatcaatgc
    18361 aaggcttgca aatttcaggc tcagcattga tgtgcgcggc catcgtggtg atgaggcagt
    18421 ggcccttgtt caaaaatata ttgacgaagc catcctgctc aatgttcctg aagtcagtat
    18481 attgcatggt aaaggcaatg gcgtcctgag gaggatgatt cgcgattacc tgcatacggt
    18541 atccgaagtg aggcagatga ctgacgaaag cctcgaaagg ggaggagccg gaattacggt
    18601 cgtcaggttc aggtaatcag gaattaactt atccaataaa gaattgaatg atgaaaaaca
    18661 ggctgtctgt taccctggta atcacgttgg tgtgctcttt tgccggcagt tgttctgatg
    18721 ggaaaagaac ggatgaagct ccggatacca tccttctgac tgtcgataac ttcgaaaagg
    18781 aggcgggaaa ggatgtggta atgatagatt tttgggcagc ctggtgtaag ccttgccgtg
    18841 gatttgctcc cactgttgag aaaattgccg ggcaaacagc cggaaaggta aaggtgggca
    18901 aggttgatat tgatgcgcag gatgagcttt ccaataagtt tcggatttat tccgttccta
    18961 cggtcattat tttaaaagac ggtgcagagg ttgaacggtt tatcggagtg caaccggagg
    19021 gcatccttat gaaagccctg ggtaagtaca ccgagtttta attacaggaa ggattttctt
    19081 tcctttaccg gaaggctccc gaccaccagc cggtaggaat catcaatcca ttccagaatt
    19141 aattcatcgc cgatcgatcc gttgatattt actgtattcc agtacttttt gttcatgtga
    19201 taaccggggg taacacaggg gtacttctcc ctgagcccgg gcgctttatc cggatcacac
    19261 ttaacagtaa tgctgaacgc atccgtcagg tcggtgagga ggaacatttt accggcaact
    19321 ttaaacacaa gggtgctttg gtcgaaggga aaaccttccg taactccttt ctttataagg
    19381 caataatccc ggagatcttc aatattcata gcatcattct tcaacaggtt caataaaaag
    19441 gaaaatggaa ggctgaattt ttttccatta aactgcaaat gtaagaaatt catttatacc
    19501 ggtttatgcc ggctgccccc gtgaatgtag ttggttatca tctcagaaaa aattatcttt
    19561 gtattttagc gcattgaaat cttcgggatg aatcaatctg aattgattat taattaaaag
    19621 aaagtctatg tcgttaagca ctaaaactac tgatctgaag cgcaggatgc gtgaagcatt
    19681 acgcggaggc ggcgaaaaag ccatcgaaaa gcaaaaagct accggcaaga tgactgcgcg
    19741 tgaacgaata attgccttac tcgatccgaa atcattccat gaatacgatt tgtttgttga
    19801 acatgcagga cgtgattttg atatgagcga caaatacctt cccggtgatg gcgtaattac
    19861 cggaacgggt acaattaatg gataccctgt atgtattttc gcgcaggact ttaccgttgc
    19921 cggtggttcg ttaggctaca tgcatgccaa gaagatcacc aaaattatgg atcatgccat
    19981 gaagctgaag gttcccctga tcgggatcaa cgattcggga ggcgcccgta ttcaggaagg
    20041 tgtgaattca ctggccggat atggagaaat attctggcgt aacacccagg catccggcgt
    20101 cattcctcag atttcggtaa tattaggccc ttgtgcaggc ggcgccgtgt actctccggc
    20161 tcttacggat ttcgtttttg tggtcgacaa tatttcaaaa atgttcatca ccggtccgga
    20221 agtaataaaa actgtgctgg gtgaagatgt atcgatggaa gagctgggag gagcacgcgt
    20281 tcaggctgaa gtaacgggga atgcccactt ttttgccgaa agcgaacagg agtgttttga
    20341 gcagattaag caactttgca gctttattcc atggaataac agcaaaaagg ccgatccttt
    20401 ccctgtaaaa ccgccacgta ccaggcagta taacatcaat aagatattcc ctaccaatcc
    20461 ccgtcagcca tacgatgtga aagaaatcat atgcgctatt gccgacgaca gcgacttttt
    20521 tgaagtgcat ggccgctggg ccgaaaatat tgttgtcgga ttcgggcgta tcaatgggca
    20581 tacggttggg tttgtggcaa accagcccct tgtgctggcc ggtgtgctcg atgttgattc
    20641 gagcgacaag gctgcccgtt ttatcaggta ctgcgatgcc ttcaatattc ccctggtcac
    20701 gcttgtcgac ctgcccgggt accttcccgg cgttgaccag gagcatgccg gggttatccg
    20761 tcatggtgcg aaaatcctct attcatacag tgaagccact gtgccgaaaa taaccgtaat
    20821 actccgtaaa gcttatggcg gcggatatat tgcgatgtgt tcgagccacc tgcgcgccga
    20881 ttttgtactg gcctggccta ccgccgaaat cgccgtgatg ggtcccgaag gcgctgccaa
    20941 tatcattttc aggaacgaaa ttaagaatgc cgagaatcct gatgaaatga gaaaacaaaa
    21001 aatcaaagaa tacaaggata aattcgcgaa tccatacgtg gcagcagctt acggttatgt
    21061 ggatgctgtt attgagcctg aagaaacccg gaacatggtc atacatgcac tccagatatc
    21121 gcttgaaaaa ggtatagaac gcccgaccaa gaaacatggg gtgccgccgt tctaattcat
    21181 ttctaagcgc acttcgacgt gatatcatca atccgtctgt aaaaatagtt gccggataat
    21241 ggaatttccc cttatccggt tgcccatcat gtgtttcgaa aacgactctc atggaagaaa
    21301 aatcaaaaga acaacagata aacccattgc ccgaggagcc ccaggctgag gaagtattac
    21361 aatcactcac cattgaggat gtccgttaca aaaccaggct gaataaaaaa tttctggccc
    21421 gcaagaatta tcagccgcgg gatccgaaag aaattattgc tttcattccg ggaaccgtta
    21481 taaatgtatt tatcaaaaaa ggcagcaagg tgaaacaggg cgaaaaattg ctcgaacttg
    21541 aggctatgaa aatggtaaat acgctcttcg cccctgccga cggggtagtg aaagaccttt
    21601 tggttaaaac cggcgacctc gtttccaaga accaggtgct gatgcatttt aaataatcct
    21661 tactgcctgg cttcgctgat caacgggccg aattcctggt tgcatttctt gataaactca
    21721 agtatttcgg cttttccctt acctgataag gatgaagtaa taaacattgg aggaagttct
    21781 tcccagtcct tttctaattc cttcctgaaa gctgccacat tggaatgtac cttgttatgg
    21841 gaaatcttat cggttttcgt gaaaacaagg caaaatggca ggacatgctg acccatccag
    21901 gtgataaagg ccaggtcgct cttctgaggt tccagcctgg agtcgatcag aacaaaagtg
    21961 taaagaagag actgccggtg caggatataa tcatggataa gatgttccca gcgttcacgc
    22021 tcggtttttg atattttggc aaaaccatat cccggcacat caaccagata ccaagaatct
    22081 tcaaccatat aatagttcag cagcctggtc ttgcccggcg tggccgaagt gcgcgccaat
    22141 cctttaaccc ccgtaagcat attaatgagg gacgatttgc ctacattcga ccgtccgacg
    22201 aaagaatact cggggtacct ctcatgcggg cagccattgc ggtcgttggc gctcatcacg
    22261 taactcgccg gttgtgtgct catatgaaaa tgaatttaag ctggcagttt aattttagca
    22321 aattgtaacg gagaacctgt caacagtaac agttcacctt catcctagct tcgcggggat
    22381 ggagtgatat gccgttcctt tttaatatca tagacgtcat caatggtaac caggatgcct
    22441 gtttcttcca catttccgaa accgggattg agacttgttc cgaaacaaag catggttgac
    22501 gagaggctca tataaacatt gaccagcgga ggtatgtttt cattgcaccg gcgcacctcc
    22561 tgtacaagca gcctgtaatc tttatcatac tggcctgctg tgaagaacga ttcaaggtcc
    22621 tctaccggtg tttccagttc cagttcctgt ttgggatata ccagattttt atgatcgttg
    22681 aaatacttat tcatgaaatg aagaatgaga tcacgtgcgc gggtgttgta atggggatac
    22741 atggtgatct ttccgaaaaa gtactttaca tcaggattct gcacgatgat agctcccaag
    22801 ccattccaga tgttgtcaag ggcatacatg cctttcctta cgtcaatatt cggctggtaa
    22861 gccggctgaa caaacgacct cccaagttcg atggtccagg gaagataatc tttaacaaag
    22921 gtatcggata gtgtaaacag ttcagtggtt ggagtatcca cctggccatc cggcctgatt
    22981 cttaattctt taccgtgtat aaacctgtat ccgccgataa tttcctgttc attcggatcc
    23041 caaacgaaca actgtttgaa aggcacgtcg ggatctttat cgaactgatc aaggtcacaa
    23101 tccaaaccgg ttccgcctcc ggcatcccgg aatgtaatct cgcgtaaacg gccgatttcg
    23161 gttactacat tgggcgaatt atgccaggtg acaatataaa tttcattgtt gccaaaattg
    23221 gtgtatttga caaacttatc aggggttaat tcgtcaataa taagctttct atctacgggg
    23281 gggattattt ctaacatagc ggggttgcag gtttaggcgt taaatgcttt cttcaaaggt
    23341 taaatcgggc gttttttcca ggcgataaac atgctctttc ataagttgcg cccattccag
    23401 atcggtatgc cgtttatcaa agatttgcca gggaaccggt ttgccgaaga tgatggtaag
    23461 cgttttatca tgttgtttgt acatctcatc aaccaggtag agcatttcaa tgtttgcttt
    23521 gatacccagc cggttccgga agcgcgaaag attgtaaaac caatttgaat tacgcccgtc
    23581 gatgtgacag ggaattatat cgcgcctgtg ctttcgggct ttggttatga agcttttttt
    23641 ccattcaaga tcactgatgc cccctgactg cttgcgggaa cataagcctg ccgggaaata
    23701 caggattgat ttatccgacg cgaatgtatc gtcgattatt ttcgcgttct ctgcgttact
    23761 gccgtgcttg ttgataggaa taaaaaggtt tttgatgggc ggcaaattca ggagaatgtc
    23821 attcaccggg aatacgatgt cgggcctgaa ttttccggca acatgcatga gggcaagccc
    23881 gtcaaggcct cccaggggat ggtttgatgc aatgataaat cgcggggtgt tttcgatatt
    23941 ttcagccccg gtaaaattta gtttagcgcc aaattccgtc agaatggccg cgatgaaatc
    24001 aaggcctgtt ttatccctgt tccggtaaag tgccttattt atttcatcct ggtggataat
    24061 ccgtttcagg taagacttaa taaaccgcgg gagggcctta ttaaggctct cgcttttgct
    24121 ttttataacc ctttcaaggt caataaagcc gggcgtaatt tcggttccgt tatttacgtc
    24181 atccatcaga ctcttatttt catcttcatg tctccggcat ccggtttagt tccacaaatg
    24241 tagctaaaat cacgattaat gctgcaaatg ctgcaccatt tcatagcatc gcagacaaat
    24301 gccgttcggc tataaatggt catattgttg aagcccttgg tttttcgatc ttccggactg
    24361 atataaaacc tttcttcctt gttatcaaca tattttgaaa ttgttaataa aaaaatgtgg
    24421 tagtaagtgt gggtaagtgg gaatctgtga ttaaatttac aagccaaaag aggaaaactt
    24481 atatgcctga tctgataggt gttcatgaat gcaaagccga cgcaaaagga agagttcttt
    24541 ttcctgttgc gttgaagaac cagttactgg atgtgctgga gcaaggcttt gtgattaaaa
    24601 ggagcatttt tcagcagtgc cttgagttgt atcccaggcc ggaatggaat atggtaagcc
    24661 gggatgtaaa caagctcaac cgttttgtga agaagaacaa tgatttcatc cgggtattca
    24721 tggccggcgt gcgtgaagtg tcgctcgacg gtaccggcag gattcagatt ccgcgtgacc
    24781 tggtaaactt tgcaggtatc aaaaacgata ttgtcctggc ttcggccgtg aacattattg
    24841 aggtttggga taaagacaag tatgaggctg ctatcggcag cagcatggag gatttttcaa
    24901 ccctggccga agaagtgatg ggaagtattt cacctactga atgacaacgt tatgtatcac
    24961 ctacctgtta tgcttagcga aagcatcgaa ggcctggcaa tcaagccggg aggcatttat
    25021 gccgatctta ccttcggggg cggggggcat tccagggcta tactctcaaa gctgaatgat
    25081 cagggcaggc tcatagcttt tgaccaggat gaagatgccc ttaataatgc cattgatgat
    25141 gaaaggttcc tgcttattaa ccagaatttt cggtatttcc ggaacttttt acgcatgcac
    25201 aaagcattgc cgctcgatgg tattcttgct gacctgggcg tttcttcgca tcagatagat
    25261 actcctgaac ggggatttgc cacccgtttc gacgggccgc tggatctgcg tatggacagg
    25321 aatgcaccgt tcacggcgga acagctcctc aattcatatc ctgaagaaaa gctaaaatca
    25381 gtttttgctg aatacggcga gatagcaaat gcgaggcaac tggcttacgt gattacacaa
    25441 tcccggccgc tggcgaccat cgcggaattc aaaaaggcca ttgcgtcatg catcccgcgg
    25501 aaaacggaaa ataaatatct ggccatggtt tttcaggctt tacgcattga agtgaatgat
    25561 gaaatgggag tgctgaaagc aatgctcaaa caaactgcgg aagcacttaa acctggtgga
    25621 cggcttgtgg ttatcgcata tcattcgctc gaagaccggt tggtgaaaaa ctatatcagg
    25681 accggaaact ttgaaggcgt acccgaaaaa gacttttacg ggaatttgca gtcgcctttc
    25741 ctggctatta cacgcaagcc tcttacaccg ggcgccgggg agcttgaaag caatccgcgg
    25801 tcgcgcagtg caaaactgcg tatcgctgaa aggaaataag gatggacgaa ccgaaaaacc
    25861 aactcaacat tccttcgaag gaagaagatc caaagcctga aaagaaggct aagcggaaat
    25921 tctcattgaa agtgaagaat atcctggatg gcaatttcct tacgcgggaa agggtgatag
    25981 gattgcttcc gtacctgggg tttgtgacaa tgctgggcgt ggtgctcatt tttaatacca
    26041 actatgccga taaaacgatc attgagatcg gaaaaacaaa gaggatgaat gaagaactga
    26101 ggtacgaata tatagttacc aaatcgaagc tgatgcaact ctcgaggcag agccagcttg
    26161 ttaaaacgat tgcctcaaca ggattaaaag agtcaaccgt gcctccaaga aaaatactga
    26221 tcgaaaaagc agaagagtaa aagtaacccg gaaatcagga accaagtgac ggatactaaa
    26281 aaggacatat tgtggcgggt ataccttgta tatataggga tgttcgtgct gggccttacc
    26341 atcatcgggc gcattgttta tatccaggtt gccgaagggg ataaatggag ggaaaaagcg
    26401 cggaaactat ccctgaggta tttcaacatt gaagcaacac ggggcaatat ttacgacgcc
    26461 aacggcgaat tcctttccac gtcggtaccg atttttgaag tccgcatgga tgtggcttcg
    26521 ccctatatta cagatgatat tttcaaaaag gaggccgatt cgctggcata caggctttcg
    26581 gattttttta aagaccgttc gaaacgcgaa tataaaactc tgatcacaag agggcgcgct
    26641 gaaaagaacc ggtatcttct cctgaaaagg aatgtgaatt atgaccagtt aaaaaccctg
    26701 aaaacatttc ccattttccg gaacgggaaa aacaagggcg gattgattgc aaaagcagta
    26761 gaacgccgta ttcaaccata cagccacctt gcttttcgca ctattggctg ggataaggaa
    26821 ggcaaacaga atgacctggg cctggaggga gcctatagcg atgaactgga aggcgaaagc
    26881 ggaaagcggt tgatgcagcg gatagctaat ggcaactgga ggcctttgaa caatgaaaac
    26941 gaaattgatc cccgaaacgg cagcgatatc gtcagcaccg ttgatataca tctgcaggat
    27001 gttgctgagg atgcactgat gaagcaactc atagccaatg atgccgacca tggctgtgcc
    27061 atactcatgg aagtaaagac gggccgcgtt aaagctattg caaatctcgg aaagaatgct
    27121 gacgggcaat atgaagaaaa atacaactat gcgatagggg aagcttccga accgggctcc
    27181 acttttaaat tatattccct gctggcagcc ctggaagacg ataagaccga ccttgatgaa
    27241 aagattgact gcaacggggg catcgcgtat tacgccaacc gggcgatgaa agattcgcac
    27301 ctcggaacag gcgtcctcac agtcaagcaa atttttgaaa aatcatcaaa cgttggggtt
    27361 tcaaaagtta tctacaaagc ctatgcttcg aatcctcagg cttttatcga caggctttac
    27421 agcttttcgg tgaataaacc gctgaacctg gaaattgccg gcgaaggtaa accgcggatc
    27481 aaagacaccc atgcccgctc ctggtccaag gtttcgcttc cctggatgtc gatcggatat
    27541 gaagtagccc tcactccgct gcaaatactg actttttaca atgccgttgc caacgacggg
    27601 gtgatgataa aacccaggtt tgttgatgag atcaggcttt cggacaaagt gattaaaaaa
    27661 ttcgggccgg ttatcatcaa cccgaagatt gcttcaaaaa aggcaatcca taaagccaaa
    27721 ataatgcttg aaggcgtggt acaggaagga accggcactt cgctcaggaa cccggtgtac
    27781 aaagtagccg gtaagactgg cactgcccag attgccatga acaatgcggg atacaacaaa
    27841 tcgagttata aaggcacatt cgttggatat tttccggccg ataacccaaa atactcgtgt
    27901 atcgtggtga tcaacaaccc caccaaaggc gtctattacg gcggggctat ttcggcgcct
    27961 gtatttcgcg agatagcgga ccgcgtatac gctgccgata tggaaatggg attgacctgg
    28021 gacgactcgg tgaagataac tcaccgcgtc ccctttaaag gcggttttgg ccgggatatg
    28081 gccaccattt gccgctggat gggctataac atgcaacatt acggcaacaa ctggataacc
    28141 agcgacagca gcggcagaaa aaccgttgct atgaactata agtcaggaat tgtccctgac
    28201 gtatgcggaa tgggaatgcg cgatgcaatg ttcctgcttg aaaagcaggg atataaagtt
    28261 cgtgcaacag gagtcggact ggtaagaagg caatcgcttc aggccggaac cagggccagg
    28321 gctggcagtc tggttgaact taaccttgat atttaacaaa tagatattca gtgcaatgaa
    28381 acaactcagc gagatacttt cggatgtaaa aaccctgtcg gtaaagggta accgcgacgt
    28441 catggcaggg gatgttactt tcgattcgcg caaggtgaaa gccggaagcc tgtttgttgc
    28501 cgtgaaagga acccataccg acgggcacga ctatatccct gctgtgatca gcgcaggcgc
    28561 agttgtggtg gtttgcgaac gtctgccgga aacacttgat ccgaaggtga catacgtgca
    28621 ggttgccgat acaagttttg cacttgggca gattgcctca gcctggtatg acaatccttc
    28681 ctcaaacctg aaactggtcg gcattaccgg caccaacgga aagaccacga cggttaccct
    28741 gcttttcaat cttttcagag aagcaggtta tcatgccggt ctgctttcga ccattagcaa
    28801 ccggattgat gatgaagaaa ttgcttcgac ccataccacg cccgatgctg ttgaactgaa
    28861 cagcctgctt tcgcaaatgg ttgatagagg ctgcgattac tgtttcatgg aagtaagctc
    28921 acattccatc gtgcaaaacc gtattgcagg gctgactttt gccggaggta ttttcagcaa
    28981 tattacccac gatcatctcg attttcatgt cactttcgac gagtatctca aggctaagaa
    29041 gcgcttcttc gacgagttgc cttcgggggc atttgcactt acaaaccttg atgaccgcaa
    29101 cgggagagtg atgttacaga atacgcaggc gctgaagaaa tcatacagcc tgatgacgat
    29161 ggctgatttc aaaggccgta tcatcgagac tccactcgac gggttacacc tggaaattga
    29221 cgggaaagaa atctggtgca ggctggtagg gcagttcaat gcctacaaca tactggcggt
    29281 ttattcagcc gctgtgctgt tgggagaaga gccgttcgat gtgcttacca tacttagccg
    29341 gttgggctca gtagaaggaa ggttcgatta tatgaaatca tccgacggga ttattgccat
    29401 tgtggattac gcccacactc ccgatgcgtt gcaaaatgtg ctggacacga taagcaacct
    29461 gagaacagga aacgaaaccc tgattaccgt ggtgggatgc ggcggcgacc gtgacaggac
    29521 aaaaaggccc ataatggctc aaattgcggg ccgcatgagc gacaaagtca tcataacatc
    29581 agataatccc cggagcgagg agccggaagc tataatcagc gaaatgctgg caggtatcga
    29641 aggtgagtat tcacgaaaag taatcaagat cacggatcgc cgcgaagcca tcaaaactgc
    29701 atgtgccatg gcccgtccgg gggatgtgat cctggtggcc ggcaaaggcc atgagaaata
    29761 ccaggagata aatggagtga gacatcattt cgatgacaag gaacaactgc gggaattcct
    29821 gcagtaacgc taaccaaagg cacaaacaaa caatttaact aacccgcaat gttatactat
    29881 ctctttacat atctcgataa ggcctacgat gttccggggg ctggaatgtt ccagtacatc
    29941 tctttccggg cttcctgttc cgccatttta tcgctgataa tctccttgct ttacgggaag
    30001 cggattataa attacctgag gactaaacaa ataggagaaa ccattcgcga acttggcctc
    30061 gaagggcaaa gtgaaaaggc cggcacccct acaatgggcg gtcttatcat ccttggagcc
    30121 atccttattc cgactctgct ctttgcccgg ctcgacaatg tatatgtgct gctgatgctc
    30181 tttgccacga tatggcttgg cctcatcgga ttcatcgatg attacatcaa agtgttcaaa
    30241 aagaataagg aagggctggc cggtaagttt aaaatcctgg gacaggttat cgccggaatc
    30301 gtaatcgggt gcgtattata cttcaatggc agcgtggtta tcagggaaaa gctgccggag
    30361 ccgaagatca ggacgacctc ggaaatcaca gcgtttgaac gcgaatcgag ccacattacc
    30421 aagttctcgc caacaccggc caaatcactc aaaacaacaa tacctttttt caagaataac
    30481 gaattcgatt attccgtgct catcggctgg gcaggtgaaa aagcaggaaa atatgcctgg
    30541 attatcttca tcgtcattgt taccttcatc attacagcca tttcgaacgg ggcaaacctc
    30601 accgacggca tggacgggct tgcgacaggc acatcggcca taatcggaat cacactgggc
    30661 ttgctggcct gggtaagcgg caacattgtt tttgccgatt acctgaacat catgtatata
    30721 cccagggttg gtgaactggt aatttatata agcgcttttg taggggcctg tacgggattc
    30781 ctctggtata actcttaccc ggcacaggta ttcatgggcg acaccggaag cctggctctc
    30841 ggcggcgcca ttgctgcttt tgctttcatg atccgtaaag agttcctgat acccattctt
    30901 tgcggaatct ttattattga aaacctgagt gttgtgatgc aggtcgcctg gttcaaattt
    30961 accaagaaaa ggtatggcga aggcaggagg atatttctga tggcccctct gcaccatcat
    31021 taccagaaac tgggttatca tgaatcgaag attgtgctgc ggttcctgat cgtcaacatc
    31081 atgctggcaa tactgacttt catcacatta aaaatcagat agaaaaaatg agccggaaaa
    31141 ttgccatact gggagcaggc gaaagcgggg tgggagctgc aatactggct caatcgaaag
    31201 gaatggaaac ttttctttcg gataacggaa aaataaaaga aaaatacgct gacgttcttt
    31261 caaatagcgg gatagcctat gaagaaggaa agcataccat tgaccggatc ctcgacgccg
    31321 acgagatcat aaaaagtccg ggtattcctt caacggctcc gattgtaaaa caagccgaag
    31381 ccaaaggaat accggtgatt tcggaaattg aatttgcggc aaggtacacc cgggcgaaga
    31441 aaatttgtat taccggaagc aacggtaaaa caacgactac ccttctcaca tatcacatcc
    31501 tgaaaaatgc agggctcaat gtcgggttgg ccggtaatgt aggtaagagt tttgcccaac
    31561 aggttgccga aagcaatttt gactggtatg tgttggaaat cagctcgttt cagcttgata
    31621 acatgtatga tttcaaggct gagatggcgg ttcttaccaa catcacgcct gaccacctcg
    31681 acagatacga tcacgatttc aacaagtaca tcgaatcgaa attcaggata atccgtaacc
    31741 agactgacca ggatgccttt atctgcaacc ttgacgatga agttactgct gaacagatca
    31801 gccggcggca gattgccgca agatgttacc ctttcagcta tgacgggaaa aaaggcccgg
    31861 aaatagcctt tgttgaccga aataaactaa tagttaccat aaataacgac ccctttatca
    31921 tgacattgga agaattggcc cttcaggggc gccacaacat ttacaattcg atggcggcta
    31981 ccgttgccgc ccgactcact gaaatccgta aagagagcat caagcaatcg ctgagcgatt
    32041 ttcagaatgt tgagcatcgc ctcgaatatg ttgcgcgtat tcacggcatt gattttatca
    32101 atgattcaaa ggccacgaat gtcaacagta cctggtttgc tttggaaagt atcgacaaac
    32161 ctgctatttg gattgccgga ggtgtggata aagggaatga ttacagcaag ttgttcgatc
    32221 ttgtccggtc gaaagtgaaa gcgctgatat gcctgggaac cgacaattct ctgatcatcg
    32281 aagcatttac aggcaaagtg ggggccatct atgaaacgga aagcgccgaa caagcggtga
    32341 tgctggccta ttccatcggt aaaaccggag atgtggtttt actttcaccg gcttgcgcca
    32401 gttttgacct tttcgaaaac tatgaagacc gcggcgaaca gtttaaaaat gccgttcatc
    32461 gcttataaat ccgataaaaa tagtaactga cccgtcaatc gttgatgtta aactgccaca
    32521 atggccaaac tgctgcaaaa tattaaaggt gataaggtaa tctggggagt ggtgatcatt
    32581 ctcacggttt tctcaattct tgccgtatac agttcaacca ccacactggc gtatcgaaca
    32641 agacagggaa acaccgaata ctacctcatc aaacacctca gcatcctggt gtttggtttt
    32701 atcctgatgt tcctggccca taaggtgaag tacatctatt atgcccgcat atcccaactg
    32761 gccatcatta tttcggttcc gttactgctc tatacgcttc ttttcggcgc ttccatcaat
    32821 gaagcttccc gctggacaac gctgccgggt attaatttaa tgtttcagcc gtccgatttt
    32881 gccaaactgg ccctgatcat gtttgttgcc agaatgttgg caaaaaggca agaaaatatt
    32941 aaggatttta agtcggcgtt catacccatc ctgacgccgg tattgctgat ttgcggattg
    33001 atacttccgg caaatttctc aaccgcggcc ttgctttttg taacctgtat cgttttaatg
    33061 ttcatcggtc gtgtaaacct gcgctatatt ggcgcgatga tcggtatcgg tggggtagtt
    33121 ctggctcttt tcctttcgtt cctttatttt tcaccaaaat caagcggccg gttaggtacc
    33181 tggcaaaacc gcatcgaaca ctttgttgac ggcgacagcg aggaaaacta tcaggtggaa
    33241 caagccaaga tcgctattgc gaacggcggg cttttaggga aactgccggg taacagcatg
    33301 cagaagaatt ttctgcccaa tcctttcaat gatttcattt atgccatcat catcgaggaa
    33361 tacggactgg cgggagccgt gatcgtttta ttgatgtacc tgatcctgct ctaccgggga
    33421 atccggattg tatcaaaatc gccgggcaac tacggcgctt tcctggcgat cggagtaaca
    33481 ttcagccttg tatttcaggc catgatcaat atggccgttg cggtgaacct tgtgcctgtg
    33541 accggacaac cattgcccct ggtcagtatg ggcggcacca gtatctggtt taccagcatt
    33601 gcgcttggta taatactgag tgtaagcaaa gaacaggaat caggagatat gccaaccaac
    33661 gaacttcaaa atgcagaagc ctaatcatat aaaagtcatc ctgagcggag gcggaaccgg
    33721 aggccatatt ttcccggcca ttgctattgc caatgccatc aaggccaggg tccctgatgc
    33781 cgtgatcatg tttgtcgggg ccaaaggccg tatggagatg gagaaagtcc cttcggcagg
    33841 ttatccgatc aaaggggttt ggatcagcgg gctacaacgc cggttgacca tcgataacct
    33901 ttctttcccg ttgaaggtgg tttacagttt tgtgaaggca ggaatcatta tccggaagtt
    33961 taagcccgat cttgccatcg ggacaggcgg ttacgcttca tggcctaccc tgagcgctgc
    34021 ggcggctctg ggcatcccca cagcaattca ggaacaaaat tcattcccgg ggatcaccaa
    34081 taaaaaactg gcccggaaag cgaaacggat ttttgtggca tacgaaggaa tggagaagtt
    34141 ttttccggca gccgggattc gcctggtggg gaacccgata cgccaggata ttgcctttag
    34201 ttcagtaacc cgcgaagaag cagcttcctt tttcggcctt gatcctgcaa aaaaaaccct
    34261 gcttgtcatc ggcggaagcc tgggagcgct caccatcaac gaaagcatcc gcgagagcct
    34321 gcctttattt gccggcaaca acatccaggt gatctggcag accggaaaaa aatacgccgg
    34381 aaaagcggct ggcgcagttc tcaaggacgg aaaagctaca caaattacaa tgccttttat
    34441 ctcccggatg gatatggctt atgctgccgc cgatttcgtg gtttcacgcg ccggcgccat
    34501 tgccatttca gaaatttgtg ctgccggcaa gccgtgcatc ctggttccgt cgcccaacgt
    34561 agccgaagac catcaaacca aaaacgccat ggcgctggct tctaaaaatg cagccctcgt
    34621 gatagccgat aaagatgctg ttgaagaact tggcaaattg gttgtcagcc tgtcggctga
    34681 tgaaaccaaa caggaagagc ttcataaaaa cattacaaag atggcgttca gggatgccgc
    34741 tgctcagata gcttccgaag cgctggaact tattaaaaag gagtaggaat tattaaaaca
    34801 gaatacattg gatatcagaa aggttgaatc ggtttatttt cttgggatcg gcggcattgg
    34861 gatgagcgcc ctggcgcgct atttccggag cctggggaaa acagttgcag gctacgaccg
    34921 cacacgttcc ctgctgacgt cagcccttga gaaggaaggc atttcggttc attatgaagc
    34981 agataccagc ctgatagagc ccggtgtcga tctggccata tatactcctg cagttaaacg
    35041 cgaccatccc gattatgatt tttttgaaaa agcaggaata ccaatgttca aacgggctga
    35101 aatcctcgga tttatagccg accagtacaa aaccattgcc atcgccggaa cccacggaaa
    35161 gacaaccaca actacgctta ccgcgcatat cctcacccaa tcgcaggtga aatgccaggc
    35221 tttcctcggc ggcatttcgc ggaattacga cagcaacctg ctgctggctc ccggatcgtc
    35281 ctatctggta gccgaagccg atgaatttga ccgctcattc cttcatctcc accctcatat
    35341 tgcagtgatc accagcatgg atgccgatca tcttgatatt tacggaaacc ttgcatccat
    35401 gatcgagtcg tttgccgcct atgccggtaa tatccggaac gggggaatgc ttatcctgaa
    35461 gaaagggttg aaccttccct tgcaggcccg ggagaaggtt gccgttttac gttactcatc
    35521 cggcgagccg tgtgattatt atcccgataa catcaggctg gaaaagggcc tgtatgaatt
    35581 cgacctgcat catccttcag gcgtgattca cgacctgatc ctgggtattc ccggatggta
    35641 taacattgaa aatgcggtag ccgcctcggc atgcgcgtta ttaaacggcg tgactgagaa
    35701 tgagttacgc gctgccttaa actcgttcag aggagtggaa aggcgtttcg atatccggct
    35761 gcaggaaagc ggcctggtgt atcttgatga ttacggacat caccccgaag agttaaaagc
    35821 ctgtataaca tcggcaaggc aaatgtttcc cggaaggaag attaccggca tctttcagcc
    35881 gcacctttat accaggacgc gtgattttgc cgatgatttc gcccggcagc tcagccttct
    35941 tgacgaggtg atcctgttac cggtttaccc ggcgcgtgaa gaacccatcc cgggtataaa
    36001 cgctgaaatg ctccttggaa agatcacggc cgggaaaaag aaactggtgc aaaaatcaga
    36061 attgccggcc tgcctcgacg gttcttcact cgaagtgtta attaccatgg gtgcgggcga
    36121 tatcgaccgg ctggtattgc caatcgaaaa ctatctgaga ggctttattc aaaacaggaa
    36181 taatcaggaa gccaggctat gaagatgttt ctgaaaatat tgctcatcat aacatggatc
    36241 gttctcgctg cgggggctgc agtgcttatg ggcttcgcca atcaaagcca taatcagcaa
    36301 cggtgcaccg gcattaccgt aaatgttgat tgcaatggca acgagcaact cgttacaccg
    36361 gaaatcatca aatcacaact taaaaaagaa ttcgggaagt ttgagaataa actcatcggg
    36421 gacatctcaa tcgaggcaat caaccgtttc ctgggaaaga accagtacat cgatcacagt
    36481 gatgtccatc tgacggtgga agggcaactg gtagccgacc tttcgcaatg cgttcctgtc
    36541 ctgaggatcg tcacactcac ggggcaaagc ttctatatcg acaacaaggg taaagtcatg
    36601 cgtgccgatc ctgatttccc tgttcgtgtt gtggtggcta ccggagcgat cgctacggga
    36661 acaaacccga tcggtaaaaa tatctcttca tggacggaag gcaaaaagaa catagccccg
    36721 gagatattaa acctgagcaa ggcttttcag gtcgctgcgg ccatatcaaa cgacagtatc
    36781 atgaaggcgc ttgccgaaca aatttacgtt agccccgaag gtgaaataaa actgcttaca
    36841 aaagccggca gccatatcgt tttaatcggc gatgcttcaa accttgaaga gaagtttgaa
    36901 aagctggagg ccttttacag gtcaggactc ccgaaaatcg ggtggaaccg gtataaaacg
    36961 ataaacttag cttacaaaaa ccaggtaatc tgcacaaaat aaaactctat ggaatcggat
    37021 attattgtcg ggctcgacat tggaaccacc aaaattgccg ttattgtcgg gcgaagaaat
    37081 gaactcggta aaattgagat actcggacac gggcgcacag agtcgatagg cgtacatcgg
    37141 gggctgatca caaatatcag cgaaacggtg caatcgatac aacgtgctgt agcagaagct
    37201 gaacagcgct cgggtgttac cattaagacg gtaaatgttg gaatcgccgg gcagcatatc
    37261 cgcagtatcc agcacaaggg gtatatcata cgccgcaatc ctgaagccga aatcaccgag
    37321 gaagagctcg acaaccttac caacagcatg tataatctgg ccatgaatcc cggtgagaaa
    37381 atcatcgacg ttattccgca ggaatatacg gttgacggcg aaagcgggat caaagagccg
    37441 aaagggatga tggggcatca gttggaagcc aattttcaca tcattaccgg acagatgaca
    37501 gccgcgatga atatcaggaa atgcgttgaa agggccggct attttgtcga gaacatgatc
    37561 cttgagcccc tggcttccgc cgaatcggtc ctgagcgagg atgagaagga agccggtgtt
    37621 gttctggtgg acatcggcgg cgggacaacc gatattgcca tctacctcga cggcattatc
    37681 cggcacacgg cggttgtgcc tttcggcggt gaaatcatta cggaagatat aaaaacgggt
    37741 tgtgccatca tgaaaaaaca tgcagaggac ctgaaggtta agttcggttc cacactggcc
    37801 cgtgaaaacc gcaacgaaga agttgtcgcc ataccgggat tacgcgggcg cccccctaag
    37861 gaaataacct tgcgcaacct ggccaacatc atacaggcgc gcatggagga gataatcgaa
    37921 tatgtgtaca gtgaaatcaa aaatactggc ctggaaaagc gtttaatagg cggtattgtc
    37981 attaccggcg gcggggcgtt gctcaagcat accgagcagc ttaccgcttt catcaccggc
    38041 ctggatacgc gtatcggtta tcccaacgaa cacctggcaa gcaattcgtc cgatgaaatt
    38101 acaagcccga tgtacgcaac cggcgtaggc ctggtgatgc tcggaatgca acgtaatgaa
    38161 cgttcgcgga aacttacccc ggaagaaccg gaagccgtgg accaggataa gaaaaaaacg
    38221 aaaaaggggg tgaaaaagac cgattcccag aaatcatgga acaggggcaa aggctttatc
    38281 gattggttta aagacttttt cgacgacggt tccctgagtt gataattcac attcattttg
    38341 ttaacaaata aatgcctgcc cataaaacac tactatctta atcaattaat ttcggaggaa
    38401 taaaaatgcc agatatgctc aaattcgacc tgccaaaaga acaggccaac attatcaaag
    38461 ttatcggggt tggcggtggc ggcagcaacg cggtaaccca tatgttcaaa cagggaatta
    38521 aaggggttga tttcttaata tgcaataccg acgcccaggc attggctcaa agtcctgttc
    38581 cgaataaaat acagcttggt tcaaacgggc tgggggccgg ctcccgtccg gaagtgggcc
    38641 gtgaagcggc tatccagaaa gccgacgaaa tacgcgacat gttcagttgc aacacttcca
    38701 tgttgtttat aaccgccggt atgggcggcg gcacaggcac cggggctgcg cctgtaattg
    38761 cccaggttgc ccgcgaattg ggcattttaa ccgtcggcat cgtcaccaag ccttttgctt
    38821 ttgaaggccg taaacgcgat cagtatgccg atgccggtat tgaagagctg aaaaagtttg
    38881 ttgataccat cctggtcatt tgcaatgaca aattactgga ccttcagggc gatctgaaac
    38941 taagccaggc tttcggcaaa gccgatgacg tcctgactat cgctgcccgc ggtattgccg
    39001 aaatcatcac cgttacagga cgtataaacg ttgatttcga agatgtaaag accgtaatgc
    39061 tgaaaagcgg aaaagccatc atgggcgccg gaattgccga agggccaaac cgcgccattg
    39121 atgcagtcca gatggcgctc aattcaccgc tcctcgacga taccgatatc aaaggggccg
    39181 ataacatcct gctctatatt acctccggta aaaacgagct ttcgctcgaa gaactcaacg
    39241 agatcaccaa ttttattacc gaccaggccg gcgaagatgc aaatctgatc tggggcgaca
    39301 gtattgatga agccctcgac gatgaaatat ccataaccct cattgccacc ggtttcgata
    39361 agcgcgacag gattgtacat aacatcgacg gttccgaaca atataaatcc gtaatgaacc
    39421 tgccggtgaa taaaccggcc gccatccagg aagtcaccct cgttcccaaa gacaaggttc
    39481 ctgccgagcc tgttgaaact ccgcagcgga ttgccgttgc cacacccgag ctgacggtat
    39541 cgttgttcga aaaccctgcg atggccggta aacctgaaga catcattcct gaaaatgccg
    39601 aacaaccgga acctttgttt aaagtggcgc cgccggtcat caaggaaccc gagctggtca
    39661 ttacgaatac ctgccaggtg aacaatcccg tgaagaagga agaggcgcaa aaaaatcagc
    39721 ctgagttgga aaacattcaa tacgaccgga tttcgaagct gaaggaaatg agtatgcatc
    39781 tcaaaaggcc cggcaccatc gaagagctgg agcgtcagcc tgcctatatc cgccgcaatg
    39841 ttgagctaac cgatatgtca tcctcacagg gaagcattca gtcgaggtat acgatcggga
    39901 tgaacgacaa ccagcaaccg aacagcttaa aggaaaacaa ctccttcctt catgataatg
    39961 ttgattaaag caaaaccggt tttattatgg aacttgaaaa actgatcaac gacgacatca
    40021 agcaaacgat gcttgcgcgc gacagccgga aactggatgc attaagggct ataaaagctg
    40081 ctttgctgct cgaaaaaacg ggcaaagacc tcaaatggga tggtgagatc cctgaagaag
    40141 tggaattaaa gcttctgcaa aagctcgtca agatgcgccg cgaaagcgct gaactctaca
    40201 aaagccagaa ccgccccgac cttgccgagg aagaactttt ccaggccggc atcattgaga
    40261 aataccttcc ggcccagatg gacgaagctg agatagaaac tgttataaag gcgataatta
    40321 ctgaaactgg cgctcaaaat attaaagaca tgggcagggt gatgggggct gccaccaaaa
    40381 agctggccgg aaaagccgat aataaggcca tatcagagat aattaaacgg ctacttggtt
    40441 cataggcctt gtttacttgt aattacagac tggccatgcc gcgaaccacg gcatggcttt
    40501 ttttgcgccc cggtagccgg aatcaatgcc ggaatgagct ataaaaagtc ttttgaaggt
    40561 tcgggagtaa taatgactgg gaggcatcct gaacgccgga taaagcgtaa ggttttggca
    40621 gggacagaaa aattgggcca agtaaagagt gaaagattga taaaaaaatc ccgcccctta
    40681 tcaggagcgg gatcagcagg gaaattcttt atgcggatta agcgcctaac tgcaggcgat
    40741 tgaacttttt aacagtgagt tctttatcgg ctttctgcag gtaatcgcgg acagtaagtt
    40801 tactgtcgtt aataaattcc tggttaagaa gggtgctttc tttatagaat ttattcagct
    40861 taccgagagc gatcttttcg agcatttctt cgggtttgcc ttcggcgcgg gcctgttcct
    40921 tgccgatttc catttcgcgg tcaataacgt cttttgctac gtcgtccttg tcaacagcaa
    40981 tagggctcat ggcagccacc tgcatggcaa cgtgttttcc tgtttcgtcg aaacccggaa
    41041 cgccggcttt gttaaatccg aggatggtgg ccagcctgtt tccgaaatgg ttataagcaa
    41101 ccacaaccgg ggcttcgatg cagttataat gggctaattc agttttctcg ccggtttttc
    41161 cgatcaggtc ggtaacgcct tcggcaacgg tgcggccgtt aatggtcagg gctttgagcg
    41221 catcaagatc agccggtttg gcctggatgg caagatcgat aactttattg gcgaaatcga
    41281 tgaattcttc gttcttggcg acgaaatcgg tttcacagtt gagcatgatg atggcgccga
    41341 aagtcttgtc gtcggtcact tttgcgatca cgatcccctg gttggcgtca cggtcggcac
    41401 gtttcagggc cagtttttgt ccttttttgc ggagaatatc gatagccttg tcgaaatcgc
    41461 cttccgattc aaccaaagcg tttttgcagt ccatcattcc ggcgccggtc atctggcgca
    41521 gtttattaac atcagcagca gttatagcca ttgttttgat attttattgt tatggataag
    41581 ggacgcattg gcttgcgtcc ctcttatttt atatggttga tgtgatttcc ggaaaccgtt
    41641 agggcctttt gcggggagcg cttccgcctg gtttgcgggc aaccacttta cggggacgtt
    41701 tggcacgttc ttcactactg ccttcttcgc cggcaacgcg tttcttgtgg gcggcttcgc
    41761 gtgattcttc atccgtctct tcttcctctt catcaacatc acggatgatc cgttcgccat
    41821 gttcatcata ttcttcgtgc tcttcttcag cgcgtttatc gcggtcgagt ttgcgttcgg
    41881 caagtccttc ctcgatggct tcaatcatct tcttcacaac gagggcgatg gattttgaag
    41941 cgtcgtcgtt ggccggaatg gggaaatcga cgaggttggg gtcgctgttg gtgtcaacga
    42001 tggcgaaagt ggggatatta agcttgcggg cttcggcaat tgcaatgtgt tctttcatga
    42061 tatcgaccac gaaaacggcc gaaggcagct tgttcagttc ggcgatggaa ccgaggttct
    42121 tctcgagttt ggcacgctca cgggtgatct ggaggcgctc acgtttcgat atattgctga
    42181 aactcgggct ggccatcagc ttgtcgatcg acgacatttt ctttacggct ttgcggatag
    42241 tggcaaagtt ggtaagcata ccgccaggcc agcgttcggt aacgtacggc atattgacgg
    42301 gctttaccag ttccgaaact agttctttgg cctgtttctt ggtggcgaca aagagcactt
    42361 tcttaccgct tttggcgatc tgtttcaggg cgccggcggc ttcgtcgatt ttggcgatgg
    42421 ttttgtaaag gtcaatgata tggatcccgt tgcgctccat gaagatatag ggagccatgt
    42481 tgggattcca tttacggcgc aggtgaccga aatgggcccc tgcatccagc agttcatcaa
    42541 aattggttct tgccatttta ttattctcct gattattagt ttacattctg tagaagccaa
    42601 ttgccaggta gtccgcgcca aaggcgaagt cctggcaatt tagatactaa actcccttca
    42661 aacacatatc tgttaacgct tgctgaactg gaagcgttta cgggctttct tctggccgta
    42721 ttttttacgt tcaaccatgc gggggtcgcg catgaggaat ccttcttttt tcagcgccgg
    42781 gcggaattcg ggattgattt cgcagagcga acgggagatg ccgtgacgaa gcgcttctgc
    42841 ctgtcctttt acaccgccac cgtccatggt aacgtttaca tcatattttg caacggtatt
    42901 ggtgacaaca aaaggttgtt caaccgtatt ttgcagaatg gcagtgggga aatattcttt
    42961 ataatccctg ccattgacag tgatcgtacc atttccttct ttgagataaa ccctggcgac
    43021 ggcacattta cgcctgccga ccgtttttgt attctccatg ctggttattt aatttcgtga
    43081 atgttgatca gtttaggttg ctgagcctgg tgatcgtgct cgggaccggc aaatacgcgc
    43141 aggttacgga acagttcgct tccgaggcgg gtttttggca acatgccgcg tacagcttcc
    43201 tctaccagaa aagcaggctt tttgcgaagc atttccttag cggttccaac cttctgccca
    43261 ccgggatatc ccgaataatg aatgtactct ttttctgtca tcttggctcc ggtaaggcgg
    43321 accttatcgg cgttgatgat gatcacgtta tcgccacaat caaggtgggg agtataattg
    43381 gttttgtact tcccgcgaag gatggcggct actttggtag ccaaacgtcc caatacttca
    43441 ttttcggcat ccacaagtac ccactccttt ttaatctctc cgccttttgg ggtttgtgtt
    43501 ttgtaactca atgtattcat cggtttctgt aataagctgt ttatcaaaaa tctcctcttt
    43561 gccttaaaaa ggggtgcaaa agtaggactt aattttcgat ttaacaaaat ttgctaacaa
    43621 tattttgttg gtaaaaaaga aacaaggctt ccggggattc ccgaaagcct tgtcctgtaa
    43681 gtcggagtgg tcagactcga actgacgacc acttgcaccc catgcaagta cg
//
LOCUS       DAOPYV010000021        43199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_751_length_43199_cov_11.977193, whole genome shotgun sequence.
ACCESSION   DAOPYV010000021 DAOPYV010000000
VERSION     DAOPYV010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 43199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43199
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_751_length_43199_cov_11.977193"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..866)
                     /locus_tag="PKI35_04275"
     CDS             complement(300..866)
                     /locus_tag="PKI35_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNX43631.1"
                     /translation="MNIEQKYIDQANQLWPELEWINDPVLRKQTTNTWALALQKSVLT
                     PDDLNTIPFTLLCGPDLKVTFMDHKRCVVHVAKDAGNKMNEFFKTDLPVNMDVLIAGA
                     ILADVGKLLEYELKDGKSVQGSYGKYLRHPFSGVSLAEQCGVPAPVCHIIATHAGEGD
                     MVKRSVEAYIVHHADFMTFEPFRERLKI"
     gene            complement(996..2189)
                     /locus_tag="PKI35_04280"
     CDS             complement(996..2189)
                     /locus_tag="PKI35_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNX43632.1"
                     /translation="MAKRTIVTMPGDGIGKAVMAEALRVLDAAGFDAEYVNGDIGWEF
                     WCKEGNPLPDRTIELLKKHKIALFGAITSKPKDKAAAELAPELQGKGIVYYSPIVSMR
                     QMFDLDICQRPCRTFKGNPLNFIRRGKGGVIEEPVIDTMIFRQNTEGLYGGVEWTNPS
                     DLVYEALMTHPKFRDNFAWCPRPELAVSTRIFTKKYTARILKVAFEYAKAKGFNTVTL
                     CEKPNVIRETSGMMLKLAQEMAKNEYPGIRVQDVNIDAQMMWLTKNPETYHVIVAGNM
                     FGDIVSDGFAGLIGGLGFACSANIGPEVAVFEPTHGSAPKYADYPVSIVNPIAMIMSA
                     CMMLDHIGEEAMAERIRKAVADVVEEGKVLAYDMLKLSGSPDVINQGAASTRQMADAV
                     IAKLK"
     gene            2586..4019
                     /locus_tag="PKI35_04285"
     CDS             2586..4019
                     /locus_tag="PKI35_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNX43633.1"
                     /translation="MLNLGQTIHHKIFGIISETASALNTDAFVIGGFVRDALLGRPSK
                     DVDIVTSGDGVKLAEEVAARLMVKKVAVYRNFGTAMFNYRNWQVEFVGARKESYRGDS
                     RNPDVEPGTIEDDQLRRDFTINALAISLRPGDYGLLSDPFNGLDDLKGKQIRTPLNPD
                     ITFSDDPLRMMRAIRFATQLQFDIVPETFAAIRRNRDRISIISQERITDELNKIILSP
                     EPSTGFRLLDDAGLLELIFPQFVALKGAEYIDGKGHKDNFSHTLEVLDRISVNTGDLW
                     LRWAAVLHDIAKPVTKRFDKETGWTFHGHEFRGMKMIPGIFGHLRLPMNEKMKCVQKL
                     VQLHLRPIVLSQEEVTDSAVRRLLFEAGDDIDDLMMLCEADITSKNPEKVRRYLKNFE
                     IVRKKLVEIEEKDKLRNWQPPVTGEIIMKTFGIEPCKQVGIIKTAIREAILDGVIPNE
                     FGEAFAFMVQEGKKLGLNVVDGGGLAG"
     gene            4094..4684
                     /locus_tag="PKI35_04290"
     CDS             4094..4684
                     /locus_tag="PKI35_04290"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43634.1"
                     /translation="MQAYRFRLLFEEQEDFLRDIEIKPNQTFRELHDIIKQCVGLEGN
                     ELASFFVCDRKWQKSQEITLLDMMEAEEQAAPVANDDDESAPEFKMPVNVMDQVRLKD
                     VIEDPHQRFIYEYDFLNPKTFYLELTRILDGDPAREYPVCVRKEGAITVTVAPVVPSY
                     LDDPDEEAMMKELNDMIKDGGMEEEIDENFTTEPEW"
     gene            4691..5632
                     /gene="miaA"
                     /locus_tag="PKI35_04295"
     CDS             4691..5632
                     /gene="miaA"
                     /locus_tag="PKI35_04295"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNX43635.1"
                     /translation="MKTHAGHGPSMISGKTNHTLIVIEGPTAVGKTAFAVTLAKMLGS
                     VVISADSRQFYQELSIGTARPSAEEMEGVKHYFSGNLSIHDYYNVARFETDVLQLLPS
                     LFAENPLVIMAGGSGLYIDAVCNGIDDFPDPDPELRSYLKGLLRDEGIEKLRELLKEQ
                     DPAYYDSVDLMNPVRLQRALEVSITSGKPYSELRAGQFRKRDFRILKIGLNLPREALF
                     ERINRRVEMMMEAGLPGEAGSLLPFRHLNALNTVGYKELFEYFDGKVLLEQAVENIKT
                     NTRRYAKRQLTWFQRDKEVNWFEPSQVKDVYGWVVNG"
     gene            complement(5645..7795)
                     /locus_tag="PKI35_04300"
     CDS             complement(5645..7795)
                     /locus_tag="PKI35_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNX43636.1"
                     /translation="MKTFPSIFAGLVLLGACQSNNSLKKAYQWPDVPAPVCEKKATEL
                     VAHGDTRIDNYYWLNDYFRKGPDSSKVVKYLEAENAYLDTMMSGTKAFREKLYNEMKA
                     RIKEKDEIVPVFKNGYYYYTRMVEGKEYFLYCRKKGNLEAREEILLDVNRMAEGLEYF
                     AVSGLSVSPDNNLLAYGVDKVSRRQYAIYVKNLTTGVTTLEIATGTNGNPVWAADNKT
                     LFYVKNNPETLLSEKILRHTVGSPQAGDAVVYTEKDPSNYITVWKSKSDKYIFISSEA
                     TMSSETRFIAADSPASPFAVFQPRMKDVLYSVEFVNGKFVILTNLDALNFRLMETYPE
                     KTGRESWKEIIAHRPDVLIEGFEPFKGFMVVTERQDGLINFRVMDLVNRKEHYVKFDE
                     PAYLASSDANAEYTSRVFRYNYTSLTTPRTVYDYSMTDKSKKMMKQQEVLGDFKPENY
                     VTERLMAKASDGTEVPVSLVYRKGFRKNGKSPLLLYGYGSYGYSQDATFSSSRLSLLD
                     RGFVYAIAHVRGGQEKGRQWYEDGKMHKKMNTFTDFIACAEYLIHQKYTSPDHLYAMG
                     GSAGGLLMGAVVNLRPDLWKGIIAQVPFVDVVTTMSDPTIPLTTNEYDEWGNPAVKAD
                     YDAMKSYSPYDNVEKKAYPNMLVTTGLHDSQVQYFEPAKWVAKLRGMKTDKNILLLKT
                     NMETGHGGASGRFKYLKDIALEDAFLFALEGITE"
     gene            complement(7831..8475)
                     /locus_tag="PKI35_04305"
     CDS             complement(7831..8475)
                     /locus_tag="PKI35_04305"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNX43637.1"
                     /translation="MSSSYHLDRQYSRIGIIAVLVVAGLLFFRFINLKITERNTTIQN
                     NIILEKLRSTAKLVIWEQDFKIVNITRMEKKYFNSDFLKFTEKVSTTARGRIGFHIDL
                     GDTANTTYRVEKDRIEIHAPLQITYVSIDNSTIEQIKEASFDPTLEVDKEEIVRRLGE
                     IALRQQLKPALEEVKKLSLSKQEKSLTALTGKPVKIILTSVPSLQSGLEQIKMK"
     gene            complement(8650..9093)
                     /locus_tag="PKI35_04310"
     CDS             complement(8650..9093)
                     /locus_tag="PKI35_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43638.1"
                     /translation="MNPKPKCLYCNKSSAVAPLSESKTKLTGFASSGYYYCGEACHEE
                     ILQFDKRVKEKSARFLILMAVSALSYIPLMILSFLSAYGNIFMSLSIASPLVFIGIVM
                     IVYPFATPETNRMWGIRKSITVIKRLGYALAFSGVVAGTIIYLLA"
     gene            complement(9465..11201)
                     /locus_tag="PKI35_04315"
     CDS             complement(9465..11201)
                     /locus_tag="PKI35_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43639.1"
                     /translation="MILLKIFKSIAVELSIIFILILTYNNYIGNSDVTIKADGKGYYD
                     YLPSVFIHKDLVRKDFSRVGNSPVYQRVNKMPVYVPFKSRLVNKYSVGTSLLISPFFA
                     WTYMHELHRNNGQVDQYSKPFQISVFHAAIFYLFLSLWFLKQILKLYNIKPLIIILSQ
                     ILLTFATGVPDYAGFEASFSHIYSLFAISAFIYFTKRYFTTGKPLNFLPASAFLALVI
                     LIRPFNALIVLFVPFLAGSVNELKSGIFRIVKNPPALIGGSLIFAALLSIQCIVWYLQ
                     TGTFLLYSYQHEGFNFSSPEIFNTLFSYRKGLFVYTPVLFLCLFGLIRLAVDGRYYLA
                     LTWLFFFSAITYFISSWWCWYYGGSFGLRAFIDYYPVFFILFALFMDHLRPGIKIPLI
                     ILSLCTIPVNLIQTKQYKEFILHWSEMDREKYWKVFLKQDEEYKGLLWKKTYSYRQYD
                     TAFQLNIEKKDILPDKTDTLLLLSSNNIGNFNRVSIIQVKFSNDFNKDDRTRLMLAIL
                     DTANRKVLYNYNPPLIHFADNNLNQYQSGHFDYEFSPMADNSDKIIALKAISGQREVS
                     LKNIHISFLIKK"
     gene            complement(11465..12292)
                     /locus_tag="PKI35_04320"
     CDS             complement(11465..12292)
                     /locus_tag="PKI35_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNX43640.1"
                     /translation="MKVGILCSSNLNSFNHKVLEHIVNLHNDKLACCYIDVRPGKSFL
                     KKSVFHIKKRQGSYIFFMSAGNLFRAKQPALSAEDFCSRYNIPVFKTDDPYSERTLDS
                     IRSEKIDILVYLSGFGTIIRKPLLEIAPLGVLSYHHGNMREYRGQPAAFWELYNGEAE
                     MGATVQRINEWIDTGMPIVEKIIPIHPTDTYNILRERLYSETPEMMAEALKRYETDDF
                     RPCRLENYGTLYRIPSAKEYLILRYRVFRRRRKWLLSQASKQLMFNYVSAQQKTKLK"
     gene            12633..13448
                     /locus_tag="PKI35_04325"
     CDS             12633..13448
                     /locus_tag="PKI35_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNX43641.1"
                     /translation="MLSSSDLNNPWRKVASAIYRKPVDSKIFGSVEMDITRLEEYIRM
                     KRKQGLRITLTHVFTLAVARAIREEVPELNSYIRRGRIKQRGHVDAMVSVLLKGLQMS
                     TIKVPDADRLTLGEMALLLRNEIGNSRKGNESKTMKLKRLMAAIPWPFRNWLYRLLKK
                     ITIDWGFSLPSVGLGVDSFGSFVMSNIGSIGLDMGYPALFPVSNVSMVFILGGVSDKA
                     WVVDGQVEVRRIISLGAALDHRAVDASHGGRLFNYLKYIAKHPETLEEKPSII"
     gene            13522..14844
                     /locus_tag="PKI35_04330"
     CDS             13522..14844
                     /locus_tag="PKI35_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNX43642.1"
                     /translation="MTKYLRYVWMAAIVAAMAIIAGRHFPNYALRISFFSAFLFIDAL
                     YWPAFRKTLSPGKALAARCLLIYYWLPFVLLVATALAAVFIPVQKWFPFFRIYIPGFI
                     VVLYIGKILLLIILMLSDLLTAPYNLIVWFRSSGNKAGYEWKRFKAVAYTGLAVSFTV
                     ILIFMAGMVHWVYDFKVQNVMVEMDELPASFNGFKIIQISDVHLGGWRTKERLREAVS
                     IIRQLKPDMVVFTGDMVNFSSEEALPFTKELRRLKAPSGVYAIKGNHDYGDYLSWDTP
                     SGKDKNVRDLDAFYKNIGWQLLNNAHRLLIRGNDTIVLVGVENWSASRLWGKRGNLKK
                     ALLGAEHPGFKILLSHDPTHWDNEVSGLYPDIDLTLSGHTHAMQMGWEGRNSQWSPAQ
                     WKYNEWAGLYEHDFPVKQFLYVNPGLGHILYPGRIGIRPEITVIELRH"
     gene            complement(14879..16342)
                     /locus_tag="PKI35_04335"
     CDS             complement(14879..16342)
                     /locus_tag="PKI35_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNX43643.1"
                     /translation="MDNLSDYFSRFREGIVGNHLEYETPYGTKKLLYADWIASGRLYR
                     PIEEKLLHEIGPWVANTHTETSEAGSIMTQAYHYAHSLIKKHVNAGPQDVIITNGFGM
                     TGVLVKFQRILGLKTCGILQHSECLNKSEKPVVFITHMEHHSNQTSWYETIADVVVIP
                     PGNGLLVDPEMLRTELEKYKGRKKLIGSFSACSNVTGIETPYHELAKIMHEAGGVCFV
                     DFAASAPYVNINMHPSDPLECLDAIFFSPHKFLGGPGASGVLIFNKQLYHQGTPDQPG
                     GGTVNWTNPWGGYRYIDDIEIREDGGTPGFLQAMKTALCIGLKNEMGVENMRKREKEL
                     VEKAFKELRKIPGIHILADNVEDRLGILSFCLDHAHYNLVVKLLSDRFGIQARGGCAC
                     AGTYGHYLLDVTFEKSAQISKFISMGDLSSKPGWIRLSLHPTMTDDELDCCLEAIRQI
                     SLHHEEWGKDYSYDKHANEFRHKHDNDRKNKIIGSWF"
     gene            complement(16421..20641)
                     /locus_tag="PKI35_04340"
     CDS             complement(16421..20641)
                     /locus_tag="PKI35_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43644.1"
                     /translation="MAHFNILSLLKFSDTALFVCLALFLSASPEYTFAQASQGTGNGA
                     YAAEVKAGDDCYNTKDYGNALMHYRNAGILNPAQKYPQERIGLISDFLKENKDLKNSL
                     FEDAILKGENFYQNKDYPSAQREFRKALEIDPEAQYPKDKLALIRQVYTDPGDAARYN
                     DAMLKADKAMVAADYDQAKNWYQIALTVDPDARQPREKILEADKLKAEAAIKKSQYDK
                     FVNAADKLIQAEKRAEARAEYQKALDLLPNQTYAVQRVAEIDAWLNERKKEQEAYDRA
                     ISQADQFYISRDFTNARVKYQEALKVKPNARYPKDMLDKTSTGEAEVKSLQQRYDAAI
                     ASADNFYKSGDMDAAMLGYRSASGILPGESYPRTRIAEIEKSIGEKTSRKEAYDIAIM
                     NGDQAFQQNKPEAALAHYKNALTLLPDEKYPADKIAEINALLLERNKADESYKTAIAT
                     ADLKFREKKYEEAATGYQSALALKPGEKYPQDQLNEIDAIYKARQDKEDQYKNAVAAG
                     DRLFAEKDYNNALDSFRQAEALKPAEKYPKDKMAEINRILQEQKQLDNTYQSAVAAAD
                     KSLAAAKYTEALTGYRKALDLKPAEEYPAGKIKEIDSILAGIKLKDENYVSVISRADQ
                     LLADSKYTEAIGAYNEALQIKPAEKYPRDKIAEAESALARNKALEDNYQSALTSAEKS
                     FSDKKYPDALAGFQKASTIKPSEQYPLDKITEIKAILDDLAAKQVAYEEAVTRGDKLF
                     AAKDYEEAIASYRQALVQKPEESYPANKISEINTILNGIKATDDAYAVAIADADSKLA
                     QKQYGQALAGYNSALGIKPGETYPAEKITEINSILAGIQAVNDAYEKAITEADRYFGS
                     EKYEESIAGYNNALTIKPGEKYPADKITEAGKKIADLKARQESYEKAVAEGDRQLASK
                     AYTGALAAYNEAISILPAEKYPTQKIAEINTILDGIKAIEDKYNTAIANADAFYNQKK
                     YREALEPYESAATIKPAETYPKQQIDKINQLLAEQKKRDDDYTTLLVGADKAFAEKQY
                     HQALSGYRGALEIKPEEQYPKDKIDAINGLLAEIQARDEAYNKALAEGDAHYTSNDYQ
                     AALTSYRNALTIKSGETYPAQKIDDIEARLKETDNQYNKNIAEGDARLAAREYPQALN
                     AYQQASEIKPEEKYPREKIDEINKALALEKEQLETMYAGFITEGDRLLAGKEYPEARS
                     AYNKASGLKPAEQYPKDKLAEINKILQEQAKALKEEYDKAIVVADNFYRQKVLDQAIE
                     SYEKASAIKPDETYPGEMIRKIKQYITDHAIKEVNTGAVTISAGEEKKFSFAVIEPRL
                     RKNNYVLIHARATSGNIPKVFLNYGRDAQKNGGIVLRTISPGEGADYLVRLAGQDRWY
                     REDNNWISIYTEGSDVEISRIQISQGDE"
     gene            complement(20756..21406)
                     /locus_tag="PKI35_04345"
     CDS             complement(20756..21406)
                     /locus_tag="PKI35_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX43645.1"
                     /translation="MITASNIHKSFGSLEVLKGIDLEINKGEIVTIVGPSGAGKTTLL
                     QILGTLDRPTAGSVVLNGEAVHKLNEKKLSAFRNKHIGFVFQFHHLLPEFTAIENLCI
                     PSFISGSSRKQAENRATELLDFLHLTERANHKPSELSGGEQQRIAVARALMNHPDIVL
                     ADEPSGNLDSVNARELHSLFFDLRKEFNQTFVIVTHNKEFADMSDRKLTITDGLIV"
     gene            complement(21452..23899)
                     /locus_tag="PKI35_04350"
     CDS             complement(21452..23899)
                     /locus_tag="PKI35_04350"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNX43646.1"
                     /translation="MRQSFSLVLLLLITLTLQSQNAVSNLTSRKTAVAVRVLNAPRIN
                     GILDDTAWIKAPVTGDFKQSLPLFGHEPSFPTEVRILYDDYAIYVGALMHDPHPDSIL
                     MQLGNRDDDNLNADMFEVEFDTYNNQLDAYSFTVTSSGVQLDARKSDETYNGVWLSAV
                     RKTEKGWVAELMIPYSAIRFPRTETQCWGFQIIRTVRRYRETDQFSPEKPGAGNNLVY
                     WGNLTGISDVKPPVRLSLTPYVSVGFEHFPQTEKKDISTSFGGGTDLKYGINESYTVD
                     MTLLPDFSQVQSDNKVKNLTAFETVYTEQRPFFREAVDLYNKGDIFYSRRIGKTPMYF
                     YEAEDMAGEGYKLKKNPVQQRLLNATKISGRGKKGFALGILNAVTGNTYAIAEDTISG
                     KEKKILTDPATNYSIVVFDQALKNNSDIYLINTNVIRGKGFGDANVTAAGIQLNNKKN
                     IYQFNLNGGISQLYNQPDSSGSKSNLTLGYKYFAGLSKVNGRFQWLILHGIMDKNFNA
                     NDLGVTLYNNYSINEADVSYNLYQPWWIFRDWHNELSLENTNNHTTGLMQETELELES
                     VATLLNYLTVWANMNYTFPNGHDYYETRADNRYYIQPEALSGTLGISSDYRKPFALDA
                     GVTLRSASRDKTHGYKVNAGPIVRVNNHFMLKYEFELESVFNEHGYADTRDDGDIIFG
                     NRDVRTVANVLNCKYLFINNLSLNLSARHYWSTGKYDQYYSLLQNGNLEENAGYTKDN
                     NFSYNAFNVDMVFSWLFLPGSSMNIVWKNSILTEKSKAAENFFSNFSDTFDSPQQNNV
                     SFKILYYLDYQQLKRSR"
     gene            24002..25201
                     /gene="sucC"
                     /locus_tag="PKI35_04355"
     CDS             24002..25201
                     /gene="sucC"
                     /locus_tag="PKI35_04355"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNX43647.1"
                     /translation="MNLHEYQAKQILRSFGVPVADGIVASTVDEAVEAAAGIQQMTGS
                     RFWVVKAQIHAGGRGKGGGVKLARNLEEVRERASEILGMRLITPQTGPQGKLVRKVFI
                     QQDVFYPGPSEPKEYYISLLLNRSESKVMVMYSPEGGTDIEEVAETKPDKIFVEIIDR
                     SAGLQPFQGRNIAFNLGLSGEALKNLVRFTDGLYRTYMGADLTLLEINPVLKTSDDKI
                     IAVDCKMRLDGNALFRHPDYAVMRDIFEEDPAEVEAGEYNLNYIKLNGNVGCMVNGAG
                     LAMATMDIIRQSGGQPANFLDVGGTANAERVEKAFRIILKDRNVQAILVNIFGGIVRC
                     DRVAQGIVDAFSNIGDVDIPVIIRLQGTNAMEAKELIDKSGLAVYSAVTMQEAADMVN
                     RVIKARA"
     gene            25730..29101
                     /locus_tag="PKI35_04360"
     CDS             25730..29101
                     /locus_tag="PKI35_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX43648.1"
                     /translation="MKKVTLLLALFAFCSWQFALAQKTITGKVTDAKDGLTIPGVNVV
                     VKGTTTGTTTGSNGEYSLKVAANAQTLIFSFVGYVTQEVAINGKTTIDVQLAPTAEQL
                     QEVVVTALGITRERKSLGYAVTEVKADELSKVSPISPVNALQGKVAGLNIAPNEGGVF
                     AGARIQLRGASTLKENNMPIFVVDGVIVDNELSGGSEWGGVNWGNNLKNLNSDEFESV
                     SVLKGSAATALYGSRALHGAIVITTKKGKTEKGIGVTLSQRLNFKKAYDGPAFQNVYG
                     EGAIPGYDDRYEDMFAPQNSFLINSAGEPYIEGISGGWWVPISYGHKMDGTKIRDYEG
                     NWINYDPQPDNYLQTFQNGFQSNTNVTIDGSGDNSSFLISYSNMKDKGVYPRNTFDRN
                     SIFSKISRKFNKYLSSELGISYSNSVSKNPPKYVEQNFITGNWPRNYNTIYAQDHYKA
                     AHGGTPQEQYGDPGWATPGADIWFDVFEDTYAQNEEALRLTPKVTVNVADWFNVVFEG
                     YLNNYYTKTETKELGQGYANEGGYYSLGHSTKQQLDGKLWLNFNKKITEDFDFRGSVV
                     AEHWQTHYNYSNAWTNGGLIVPGQYSLSNSKLEAGQEAGYKDQKILQSLYAFADLSWK
                     GQLYLTVTGRNDWSSTLVYADRTGNYSYFYPSVSLSWVMTESLDLPSWISFGKLRASW
                     AHVGNDFDPYEINPGFSRIGTVNSYNGDLPMYSFKNDVMPNLNIKPEDKKSIELGAEY
                     RFLNNRFGFDFTYYKENTFNQILKIPAPIESGVNYILINAGNIQNQGVELELHTTPLK
                     INDFEWFVDGTLARNRSKIIDLYEGVDEYSLEGSWNYGNTRIASMAIVGDEYGVLVSD
                     SDPLLYNNEDDPNDPLNGLPILSWSNSTRGVYETRSYEKKVVGNMNPDFTGSVTTGFS
                     YKNFRISAMFDFSFGGDISTYSGRYGTAYGLFESTLDKRDAQSGGLVWTSPWTGNQYE
                     DGIIPEGVFKDGTIVDMKDAEGNVVHNDVSGMTYKEAYEQGMIEPTHVEYWYYQTNSW
                     GRGVINDNVLQENSYIGFRELSLTYQIPSSILEHIKISRADISFVGRDLGYLYKTLKD
                     NLNPFSVRSNRSGDAHEWQQTPYVRSLGVNLNVSF"
     gene            29139..30863
                     /locus_tag="PKI35_04365"
     CDS             29139..30863
                     /locus_tag="PKI35_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNX43649.1"
                     /translation="MKNVYKKLGLALMAISLLIAPQCKKEFADINKNPDEITDPDLTH
                     LFTDAIYKMAGDEYLQWFYNNSVYFWRFSQMTVARGGTSSDFNNTAALGGVPFYTVMI
                     DMKEIRERIDNMNDEEKAKHQALRALTYIPQVYLGIKYTDWYGSTPYTEAIEARYTGN
                     FKPVYDSQEELFNVWLSELSSSIDVLLAADNSQLSPLNQDFIFQGDWVAWARFANSLR
                     LRIAARMEHADPALMKEILGEIVSKKVDGELALIINKDQQAIWAPGASELGPGGTNSL
                     WIENYGPAQNFSKYMCSNKDPRMQIFFRKNGLSDEAITLLQASGVTVPSFATLPVNDP
                     WDRLVGAPVAPDDAGNLNYFGSTLVDSNGTQYSRLPWVEYLYFKPKQDGGTGEYMNYL
                     LGAPEVCLYLAEFIEKGYVSGIGSAQEWYEKGVRYSCENYDVRASKAQIDNYQDRKIT
                     PEAIDALLAADGVKYGTDNLEKIILQEIINLFDNPYEGIAVTRRTGYPKRSSDLWAWQ
                     PYLSSGTELKLPRRFPWGTPGDATNLANWQEALSSQGFTADVNTGDVLNAQRVWWDKN
                     SPNYGDGD"
     gene            31141..31839
                     /locus_tag="PKI35_04370"
     CDS             31141..31839
                     /locus_tag="PKI35_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43650.1"
                     /translation="MMKRYLIFFVTYFCLAFMQEAVAQRDDKSSAILGMTGGMGITRL
                     QYNSYDKSLYHFKQTASSYFGVSLEFPVPKTENRGTFYNELAFFRFKSSASLHTADSV
                     NGNPENSYYDINLEFEPNIISLAHMFRYSFTPGNFRYFVSAGIYNNFVISAVNRKTTV
                     HTLNGVAGEPYHEDAVTDFAVHGLMITAGTGFIYKNIGLELRFDPGRNFTKLYDCSVY
                     SMTFSALLHVRFNP"
     gene            complement(31885..33849)
                     /locus_tag="PKI35_04375"
     CDS             complement(31885..33849)
                     /locus_tag="PKI35_04375"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HNX43651.1"
                     /translation="MKAIRIIALSIVTLVFSLSAAYSQVNDTVWLSTLDVSMATTGWG
                     QKGVNVSCMGNPLILNGVQYRSGFGTHAPGTLYLKLGGKAQRFHAVVGVDDEEKASSG
                     SIIFIVYGDGELLCQSSKLVSGGKSEEINVNTAGIDTLLLIVDGAGDGINYDHADWAD
                     AYFLVKGKKPVAISRPVEPAYILTPKAPASPRINGPKIYGARPGNDFIYRIPATGERP
                     MKFSVKGLPKGLSLNKSTGVITGKTPEEGRYETVITAKNSKGESSRKFIIVAGKQIAL
                     TPPLGWNSWNCFACAIDQEKVKAAADAMEKSGLADYGWSYINIDDCWMIQSESKDSIL
                     GGTPRDEKGNMKTNKKFPDVKALTDYVHSKGLKIGIYSGPGPRTCAGYTACYGHEQAD
                     AKQFAEWGFDYLKYDWCDYERIAKDHSLPELKKPYIVMREALDKAGRDIVYSLCQYGM
                     GDVWRWGAEVGGNCWRTTGDITDNWSSVETIGFSQSGLEYFSGPGHWNDPDMLVVGKV
                     GWGPALHPTNLTPNEQYTHISLWALLASPLLIGCDMTQLDDFTFSLLTNNEVLDINQD
                     PLGMQAGRIIKNGNIEIWAKNLEDGSKAVGIFNRGMKQESAVIKRSDLDMNGNYTIRD
                     VWKQEDIGPLGDSYSTVIPSHGVKLLRIYK"
     gene            complement(34019..35440)
                     /locus_tag="PKI35_04380"
     CDS             complement(34019..35440)
                     /locus_tag="PKI35_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HNX43652.1"
                     /translation="MTSRRTFIKATSLAAAGIALGGYGTATGLPGRKEYISNRPAPSS
                     RRFVSQAVEARIAEIKSVIRDPELAWMFENCFPNTLDTTITYSEINGKPDTFVITGDI
                     HAMWLRDSSAQVWPYIPFIKKDEPLRKLIAGVINRQVKCILLDPYANAFLDGNQESEW
                     KSDITEMKPGIHERKWEIDSLCYPVRLAYGYWKASGDSSVFDSDWLKAMELVYQTFRD
                     QQRKTGRGPYSFKRTTSWQTDTVPGNGWGNPARPNGLIVSIFRPSDDATIFPYLIPSN
                     FFACQSLLQLSGIYGYLNLFKEAERCRALSEELTLALSNQATIQHPVYGEIYCYETDG
                     YGNRLMMDDSNVPSLLAMPYLGCIEKEDRIYQNTRRFALSENNPYYFSGKAARGIGSP
                     HTPGGNIWPMTLIMQAMTSNSPSEISDCLQMLRNTHAGTGFMHESFNKDNPADFTRNW
                     FAWANTLFGELICKVHDEHPELL"
     gene            complement(35447..37036)
                     /locus_tag="PKI35_04385"
     CDS             complement(35447..37036)
                     /locus_tag="PKI35_04385"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNX43653.1"
                     /translation="MKKLSILPFLLLTLASINSAATSIEVSGPVSGTWDADTVLVMGD
                     LLVPDGQTLTIAPGSRIICWSHFRINIQGRILAEGNSGDTILFTVRDTANFHNPSTER
                     GGWFGIRFNQTPAQNDTSRFSFCRFEFGKAFGDSVDEYGGAIYARYFGKIGITNCLFY
                     HNNSYFSGGAVYLWNSDACLRNNIFKDNYAGNPGNIYGYGGGVCAMHSSPEITRNVFL
                     RNSSTGVGGGVSFDDADPVFTNNIFENNYSALGGALGVLRCSPASAFSNLLIVNNTSL
                     FFGGGISCIRSFPVFSNLTITNNSSCYGGGIYCNDSAMPSFYNSIIHGNTGLGVSAYI
                     WDIRSAPDFYYCDVEGDTAGFEGSGGHQGYHGEYRYNIDENPGFYGAGLFPYQLTGNS
                     PCIDAGTPDATFLNIPAYDLGFAPRIWNNRIDMGAYEYNGTTGTAGNNASSDEWFSVC
                     PNPFSNSFRIDLKRNLNLKVNFRIKNLQGNVLQDICIPPGTHHYMVDCISSCTGMLPS
                     GAYILEADNGKEKSVKIILKY"
     gene            complement(37033..37845)
                     /locus_tag="PKI35_04390"
     CDS             complement(37033..37845)
                     /locus_tag="PKI35_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX43654.1"
                     /translation="MKKKISLFQTVVITELIKLRRTVAFWLTILYPAGAVLLASLFLF
                     SMRGHSNMSPVKAINNINDVTAFFLPFYVVLTISFLCQTEHRNTMFKHLISLPVPRSA
                     YFFGKLAASFILMAVSWFLIIALTYASMIILHLTNRNLMLFPGFDNGYLFTAITRTFL
                     AGTAMVVIQYLISLRLRNVVAPITIGTTLAILPIAVLFILGVTGLISNPDVLRWLPLY
                     NPYTYPYSHVFSIMRGPSYELDLFPLPSLIYFLIAIGLSVAGAYEFKNRNFK"
     gene            complement(37842..38774)
                     /locus_tag="PKI35_04395"
     CDS             complement(37842..38774)
                     /locus_tag="PKI35_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX43655.1"
                     /translation="MDNITENPFIISTENLTFAYNGQPVLNHLNLQVPGRSIFGFLGP
                     NGAGKSTTIKALLGLLKVKPGMIHIFNKELNSHRLDILQRTGATIEVPSLYDHLSARK
                     NVEITRRLRGIPAPMVDEVIETVGLTADSDRYVGQFSTGMKQRLSLALALLHKPDLLI
                     LDEPINGLDPEGIREMRNLLLRINQEQGCTIFLSSHILDEVEKICSHVAIIHLGKIIY
                     QGETSGLKRKNGSGGVLTLTTSDNPKCLQIMSETHITSLLPGAVSVSYQSQEDIASII
                     RNLVDAGIGIYAAKPEDHRLEESFFELLKQGSEA"
     gene            38992..39711
                     /locus_tag="PKI35_04400"
     CDS             38992..39711
                     /locus_tag="PKI35_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43656.1"
                     /translation="MKTNLNLIMVAALLLLSPLAVHPQVKGNPKQAIPATNENKLNAG
                     QRNVFSGSGGLTTGEAVLEGTEGNIYFGSEWPKGIINLRDGSTITERGLRYDIYADQM
                     QMTDGKDTLALAKPEEINSLSFDGHTFIYQSYECSGLVKQGYFELLVTGKSELLLKRS
                     VSFHIEGNGDGKEKYLITECYFLKKGESPAIKIMCSKKSALEVFSEHRDEIERYMKET
                     GRKVKTPEDLKVLVEYYNSLE"
     gene            39808..40779
                     /locus_tag="PKI35_04405"
     CDS             39808..40779
                     /locus_tag="PKI35_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43657.1"
                     /translation="MSEISGSLNLLQELHNCRICPRECGADRFSARLGWCNAGAGFNI
                     SSICAHQGEEPAISGKQGICNIFFSRCNLACIYCQNWQISSRKEPAAGLGMEFSEVIG
                     RIVDLLDGGCHAVGFVSPSHHVPQVKAIIEAVRESGRNPVFVYNTNGYDKAETLKSLE
                     TYIDIYLPDFKYMDASLAKEYSEADDYPEVALSALKEMYRQKGSVLLCNHDGYAEKGI
                     ILRHLILPGHTDNSIAVLRKIAGDVSSSLTISLMAQYWPVPQVASHRQLGRPITPEEY
                     ERVTSELQALGFYKGWIQELDSQNNYLPDFNQQHPFGKAVGDGDSED"
     gene            complement(40881..41348)
                     /locus_tag="PKI35_04410"
     CDS             complement(40881..41348)
                     /locus_tag="PKI35_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNX43658.1"
                     /translation="MKEKFMAEAIRLAGMNIMNENGGPFGAVVVRSNEIIATGCNRVT
                     ETNDPTAHAEIVAIRNACVALRTFNLSGCEIFCSCEPCPMCLGAILWARIDKVYFAAD
                     QHDAKAAGFDDEHFYEEISLPAEDRRLPSEQMMRPEAVKVFHMWTNSQNRIEY"
     gene            41387..41575
                     /locus_tag="PKI35_04415"
     CDS             41387..41575
                     /locus_tag="PKI35_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43659.1"
                     /translation="MKLFSTKIIHSGIREHLYLKSLLMSLPAIMTRNAGRSIRKLLQE
                     SHGCCKIAKKILTGLCGY"
     gene            41678..42697
                     /locus_tag="PKI35_04420"
     CDS             41678..42697
                     /locus_tag="PKI35_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNX43660.1"
                     /translation="MNFFFTHYKSIDGMRRILILILFLFSFIGMYAQNETEAKQMKEK
                     RQAGFCITSDAMILFNMINDYRRTNNLPLIPLSRSLSFVAMVHASDLQANNAAISECG
                     LHSWSDKGNWKPCCYARDHAQASCMSNKPKELTGYPGSGYEIAYWDEEQATPSGAMEL
                     WKSTVVSKNVFLNRDKWQSKKWKAMGVALLDGYAIVWLGDKADSLADITLCGTDSLVQ
                     LASSPVLAPVAAVNEVPLKKDIPAVKAVEKKETGPEKEKQNQKQARKNADQFYLVIAS
                     LRDEQLAAREVEKLQERGFSNATVQAGEDVYRVTIGVYATREEAQKKLNKYISEFKGI
                     WILRQ"
BASE COUNT        11050 a         9414 c        10677 g        12058 t
ORIGIN      
        1 cgttattcag gatattttca accacgttgg cgccgccgaa gtcgcgggca acgcgtgtat
       61 cgatgaatac atccacaata tcgcccggtt ttaccgaggc ttgcttgctg tgtgcagcga
      121 ttattttttc gatgatggtc atgtcttttt ttgataaagg tttatgaatg agaggcgtcg
      181 tgcaaagttg caatgggaat atttaacttt cccgggtctg gaattattca ttttcagtgc
      241 atttcaacgt cttatatcat ttcagggatc gcttgtcttc ttaacgtcgt tgcctgatat
      301 caaatcttca gccgttcgcg gaagggttca aacgtcatga aatcggcgtg atgcacgatg
      361 tatgcttcta cgcttctttt caccatatcg ccttcgccgg catgcgttgc gataatatgg
      421 cataccggag ccgggacgcc gcattgttcg gccagcgata cgcctgaaaa cggatgtctc
      481 aggtattttc cgtaactgcc ctgcaccgat tttccgtctt tcaactcgta ttcaagcagc
      541 tttcctacat cagccaggat ggcgcctgcg atcagcacat ccatgtttac cggaagatcg
      601 gttttgaaaa attcattcat tttattgcct gcatccttag ccacatgaac gacgcaacgt
      661 ttatggtcca tgaaagtaac tttcaggtcg ggaccgcaaa gcagggtaaa cggaatggta
      721 ttcagatcat cgggagtaag cacgcttttc tgaagggcca aagcccaggt gttggtagtt
      781 tgcttgcgca acacaggatc gttgatccat tccagttccg gccatagttg gttggcctgg
      841 tcgatgtatt tctgttctat attcatatcc ggtaagttac gaaggtgaat ttaaggttcg
      901 gttttgatta aaactcaact ccgcctgcct gctggcaaac aggcggagtc gggatgatcc
      961 tgccttttgt gacagcctct ggttgttata ctctcttatt taagtttggc aataacagcg
     1021 tcggccatct ggcgtgtaga agcggcgccc tgattaataa catcgggcga tcctgaaagt
     1081 ttcagcatgt cgtaagcaag cactttgcct tcttccacaa cgtcggcaac ggctttacgg
     1141 atacgttcgg ccatggcttc ttcgccgata tgatcgagca tcatgcatgc cgacatgatc
     1201 atggcgatgg ggttaacaat ggaaaccggg taatcggcat atttcggggc tgagccatgg
     1261 gtgggctcga aaacggccac ttcaggtcct atattggcgc tgcatgcaaa tccaagtccg
     1321 ccgatcagtc ctgcgaaacc atcggaaacg atgtcgccga acatatttcc ggctacaatc
     1381 acgtggtagg tttcaggatt cttggtgagc cacatcatct gggcgtcaat gttcacatcc
     1441 tgtacacgaa ttccgggata ttcgttcttt gccatttcct gggcgagttt cagcatcatg
     1501 ccggaagttt cgcggattac gttgggcttt tcgcacaggg ttaccgtgtt gaatcctttt
     1561 gccttggcat attcgaaagc gactttcagg atgcgtgcgg tatatttttt agtgaagatc
     1621 cgggttgaaa cagccagttc cgggcgcggg caccaggcaa agttatccct gaatttcggg
     1681 tgggtcataa gcgcttcata aacaaggtcg gaaggattgg tccattccac gccaccgtaa
     1741 aggccttcgg tgttctgacg gaagatcatg gtatcgatga ccggttcttc gataacgccg
     1801 ccttttccgc gacggatgaa gttcagcggg tttcctttga aagtgcggca tggacgctgg
     1861 cagatgtcga ggtcgaacat ctggcgcatg ctcacgatcg ggctgtaata aacaatgcct
     1921 ttgccctgaa gctctggcgc cagctcggcg gcggctttgt ctttaggttt tgaagtgata
     1981 gcgccgaaaa gggcaatttt atgctttttc agtagttcga tggtgcggtc ggggagggga
     2041 tttccttcct tgcaccagaa ttcccagccg atatcgccgt taacatattc agcatcgaat
     2101 ccggcagcat cgagtacgcg aagcgcttct gccattactg cttttccgat gccatcgccg
     2161 ggcatcgtta caatagttct ttttgccata ttatcaaggg tttaagaatt tattttctga
     2221 taatgataag tatgtcaaaa ttaggactta ggttttgaat agcaagcatc aaacggaaat
     2281 atttatatac aacttcactg tcaggatggt acgccggcga tacagggtaa gattctgtaa
     2341 tcaatagatt ttacaggcct tcaggaaaca agttgctgat gggaaacgat tgccgggtga
     2401 tgttttgttc tttgcgtccg acacgttttt gtctgataca taagtgcagg cgtcattttc
     2461 ctgacggaaa aaatcattat ttcttcaggc gggttcatag agcttcactg ccgggtattt
     2521 tgggttgtgg caggaattct taattttgcc ggctcaaaac gggcgcaatc cgttattaat
     2581 gtcgtatgct caacctcgga caaaccattc atcacaagat tttcgggatt atttccgaaa
     2641 cggcttctgc attgaatacc gatgcctttg tgattggcgg ctttgtgcgg gatgctctgc
     2701 tcggaaggcc ttcgaaggat gtggatattg tgacgtcggg cgacggggta aaactggccg
     2761 aagaagtcgc tgcccgtctc atggttaaaa aggttgccgt ttaccgcaat ttcggtacgg
     2821 ccatgttcaa ctaccgcaac tggcaggtgg agtttgtcgg cgcccgcaag gaaagttacc
     2881 ggggcgattc ccgcaatcct gacgtggagc ccgggaccat cgaagatgac cagttgcggc
     2941 gcgattttac catcaacgcc cttgctatca gccttcgtcc cggcgattat ggcctgttgt
     3001 ccgatccctt caacggtctg gacgacctaa agggaaaaca gatcaggacg ccgctgaatc
     3061 cggatattac cttttcggat gacccgctgc gcatgatgcg cgccatccgt tttgccacgc
     3121 aactgcaatt tgacattgtt cccgaaacgt tcgcggccat ccgccgtaat cgcgacagga
     3181 tttctataat ttcgcaggag cgcattaccg acgaactgaa caagatcatc ctttcccctg
     3241 aaccttcaac aggctttagg ttgctcgacg atgccgggct gctggaactc atcttccctc
     3301 agtttgttgc gcttaaaggc gccgagtata tcgacggcaa agggcataaa gataatttca
     3361 gccatacgct tgaagtactg gaccggattt cggtcaacac cggcgacctg tggctgcgct
     3421 gggctgccgt gttgcacgat attgccaaac ccgtaaccaa acgtttcgac aaggaaacgg
     3481 gttggacctt ccacggacac gagttcaggg gaatgaagat gatcccgggg attttcggcc
     3541 atctcaggct tccgatgaac gaaaagatga agtgtgtgca gaaactggtg caacttcacc
     3601 tgcgccccat cgtgctttcg caggaagaag tcaccgacag cgcggtacgc cggctcctct
     3661 ttgaagccgg ggatgacatt gacgacctga tgatgttgtg cgaagccgat atcacatcaa
     3721 agaatcccga gaaagtaagg cgatacctga agaatttcga aatcgtccgc aaaaagctcg
     3781 ttgagattga ggaaaaagac aagctgagga actggcagcc tcccgttacg ggtgagatca
     3841 tcatgaagac ttttggtatt gaaccctgta aacaggttgg catcatcaag acggccattc
     3901 gcgaagccat tcttgacggc gtgatcccga acgaattcgg ggaagctttt gctttcatgg
     3961 ttcaagaagg gaaaaaactg ggattgaatg ttgtggacgg gggaggcctg gcaggttgaa
     4021 aaaccggccg gttgcgggga acggattgcc gaaatttgtt gtaactttat acgccttaaa
     4081 atacttacga accatgcaag cataccgatt caggcttcta ttcgaagaac aggaagattt
     4141 tttacgcgat atcgagataa agcccaatca aacgttcagg gagttacacg atattattaa
     4201 gcaatgtgtc ggactcgaag gaaatgaact ggcttcattt ttcgtatgcg accgcaaatg
     4261 gcaaaaatcg caggagataa cactccttga catgatggaa gccgaagaac aggctgcccc
     4321 ggtggcgaac gatgatgatg aatccgcgcc ggaattcaag atgccggtta acgtgatgga
     4381 tcaggtacgg ttgaaagatg ttattgaaga tccgcatcaa cggtttattt atgaatatga
     4441 ttttctgaat cccaaaactt tttacctgga acttacccgc attctcgacg gcgatcctgc
     4501 cagagaatac cctgtgtgcg tgaggaaaga gggagccatc acggtaaccg tcgcaccggt
     4561 agtgccttcg tacctggatg atcctgatga agaagccatg atgaaagaac tcaacgacat
     4621 gatcaaagat ggcgggatgg aagaggaaat tgacgagaat ttcaccacag agcccgaatg
     4681 gtaaaggctt ttgaaaaccc atgccggcca cggcccgtcc atgatttcgg ggaaaactaa
     4741 ccatacgctt attgttattg aaggtcctac ggcagtggga aaaacggctt ttgccgttac
     4801 gctggcgaaa atgttgggtt cggtagtgat ttcggccgac agccggcagt tctatcagga
     4861 attgtccatt ggaacagccc gtccttcggc ggaggaaatg gagggcgtga aacattactt
     4921 cagcgggaat ctgtccattc atgattacta caacgtggcg cgtttcgaaa cggatgtgct
     4981 gcagctgctt ccttcgctct ttgccgaaaa tccccttgtc atcatggccg gcgggtcggg
     5041 attgtacatc gatgccgtgt gcaatggaat tgatgatttt cccgatccgg accctgaact
     5101 gagatcgtac ctgaaagggc tcttgcggga tgagggcatc gaaaaacttc gtgagttgct
     5161 gaaggaacag gatcctgcct attatgattc ggtggacctg atgaaccctg tcaggctgca
     5221 acgggcgctg gaagtatcca ttacttcagg aaagccctat tccgaacttc gcgccgggca
     5281 gttccggaag cgcgatttca ggattcttaa aatcgggctg aacctgcctc gtgaagcgct
     5341 ttttgaaaga attaaccggc gtgttgaaat gatgatggaa gccggtttgc ccggcgaagc
     5401 cggatcatta ttgcccttcc gtcacctgaa tgcgttgaat acggtgggct acaaggaatt
     5461 attcgagtat ttcgacggta aagtcttatt ggaacaagcc gtagaaaaca tcaaaaccaa
     5521 tacgcgccgt tatgccaaac gtcagttgac gtggtttcaa agagataagg aggtaaattg
     5581 gtttgagccc tcgcaggtga aggatgttta tggatgggtc gtaaatggct gatccggatg
     5641 gacatcactc cgtaatccct tccagcgcga ataggaaagc atcttccaat gcaatatcct
     5701 tcaggtattt aaaccttccc gaagcgccgc catgaccggt ttccatgttg gttttcaaca
     5761 gcagaatatt cttatcggtc ttcatcccgc ggagttttgc cacccacttg gccggttcga
     5821 aatactggac ctgtgagtcg tgcagccccg tcgtcaccag catgttgggg tatgctttct
     5881 tctccacgtt atcataaggt gaataggatt tcatggcatc ataatccgct ttgacggcag
     5941 gattgcccca ttcatcgtat tcgttggtag tgagcggaat ggtgggatcg ctcatggtcg
     6001 ttacaacatc cacgaatgga acctgggcaa tgatgccttt ccataaatcg ggacggaggt
     6061 ttacaacagc gcccattaac aggccgccgg cgctgccgcc catggcatac aggtgatcag
     6121 gggacgtgta tttctgatgg ataaggtatt cggcacaggc gatgaaatcg gtgaaggtgt
     6181 tcatcttttt gtgcattttg ccgtcttcgt accattgccg ccctttttcc tggccgccgc
     6241 gaacatgggc gatggcatat acaaaaccgc ggtcaagcag gctcagccgg ctcgaactga
     6301 atgtggcgtc ctggctgtaa ccgtaagaac cgtaaccata cagcagcaga ggcgacttgc
     6361 cgtttttcct gaatcctttc ctgtaaacca gggaaacggg aacttccgtg ccgtcggagg
     6421 ctttggccat taacctttcc gttacataat tttcaggctt gaagtcgcca aggacttcct
     6481 gctgcttcat catttttttt gacttatccg tcatggaata atcataaacc gtcctgggag
     6541 tagtcagcga agtgtaatta tagcggaata cccgggaagt atattcggca ttggcatcgg
     6601 acgaagccag ataggcgggt tcgtcgaatt ttacataatg ctctttcctg ttcaccaggt
     6661 ccatgacgcg gaaattgatg agtccgtctt ggcgttcggt tacgaccata aaacctttga
     6721 aaggttcgaa cccttcaatc agaacatccg ggcgatgggc gatgatttcc ttccagcttt
     6781 cgcgcccggt tttctcagga taggtttcca tcagcctgaa attcaatgca tcgaggttgg
     6841 tgagaatgac aaacttcccg ttgacgaatt caacgctgta cagcacatct ttcatgcgcg
     6901 gctgaaatac ggcaaacggg gacgccgggg aatcggccgc gataaacctt gtctcgctgc
     6961 tcatcgtcgc ttccgatgaa atgaaaatat acttatcgga tttgcttttc cagacggtaa
     7021 tatagttcga cggatctttt tcggtatata caacggcatc gcccgcttgc ggagagccga
     7081 cggtatggcg cagtatcttt tccgaaagca gggtttccgg gttatttttc acgtaaaaca
     7141 gtgttttgtt gtcggcagcc catacgggat taccgtttgt gccggtggca atttcaagcg
     7201 tcgtaactcc ggttgtcaga tttttcacat aaatggcata ctgacggcgt gaaaccttat
     7261 cgacgccgta agccagcagg ttattatccg ggctgacgga taatccggag actgcaaaat
     7321 attccaaccc ttctgccatc cggttgacat cgagcagaat ttcctctctg gcttccaggt
     7381 tgcctttttt gcggcagtag agaaagtatt ctttgccttc aaccattctt gtataatagt
     7441 aataaccgtt tttaaataca ggaacgatct catccttttc cttgatgcgg gccttcattt
     7501 cattgtacaa cttttcccgg aaggctttgg tgccgctcat catggtgtcg agataggcgt
     7561 tttcggcttc aaggtacttt acaaccttgc tgctgtcggg gcctttgcgg aaataatcgt
     7621 tcagccagta ataattatcg atgcgggtgt cgccgtgtgc aaccagttct gtggcttttt
     7681 tttcgcaaac gggagccggc acatcaggcc attgataggc tttctttagg ctgttattgg
     7741 attggcaggc gcccagtaat accaggcctg caaatatgga gggaaaagtt ttcatgggaa
     7801 gtgccgggtt aaggttgata atacacggat ttatttcatt ttaatctgtt cgaggccgct
     7861 ttgcagggag ggcacggagg taagaatgat tttgaccggt tttccggtga gggcggtcag
     7921 gctcttttcc tgttttgaca gcgaaagctt tttgacctct tcgagcgccg gtttcaattg
     7981 ttgccttaat gcgatctcgc caaggcggcg tacaatttct tctttatcca cttcaagggt
     8041 gggatcgaac gaagcctcct tgatctgttc gatggtactg ttgtcgatgc ttacatacgt
     8101 gatttgcaaa ggcgcatgta tttcaatcct gtccttttca acccggtaag tagtgtttgc
     8161 cgtgtcgccg aggtcgatgt gaaatccgat tcgtccgcgg gctgttgtgg aaaccttttc
     8221 ggtgaatttc aggaagtcgg aattgaaata ttttttctcc atcctggtga tgttcacgat
     8281 tttgaagtcc tgttcccaga taacaagttt ggcggtgctc cgcaactttt caaggatgat
     8341 gttgttttga atggtcgtat ttcgttccgt gatcttcagg ttgataaacc tgaaaaacaa
     8401 aaggccggct actaccagaa cggcgatgat gccgatgcgg ctgtactgtc tgtcgagatg
     8461 ataggatgat gacatttttt tggtgttgtt gaggttgttt cagttaccgg agttgagcag
     8521 gctgaatcgt ccccttatat ggtttattta atattgatgc aagttagata aatttttttc
     8581 caaagccgaa ggcagccagc aatttatcgc agccatattt cagcgccatt atgatgagaa
     8641 tgaagtccct caggctagaa gataaataat ggttcctgca acaaccccgg agaatgccag
     8701 tgcatagccg agccgtttaa taacagttat gcttttccgg atgccccaca tacggttggt
     8761 ttcaggcgtg gcgaacggat agacgatcat cacgatgccg ataaaaacca gcggcgaagc
     8821 aattgacaac gacatgaaaa tatttccgta ggctgaaaga aaagacagga tcatcaaagg
     8881 aatatacgac aaggctgaaa cggccatcag gataagaaac cgtgccgact tttctttcac
     8941 ccgtttatcg aactgcaata tttcttcatg acaggcttcg ccgcagtaat aataacctga
     9001 agatgcgaac ccggtcagtt tagttttact ttcggataac ggagctacag cagaactttt
     9061 attgcagtac aggcatttgg gtttgggatt cattaagtta tattcagaga caggttataa
     9121 tattgcataa agatagaaaa accgggtata attcgtaata ttttatgtga attgagggta
     9181 gaaatatatc cgtgaaacct taggataatc ttaccacgga gccacgagag caaacagaac
     9241 aaagagaatt acgtcattct tgctgacctc gaagcctctg tggttctctt ttcttttacc
     9301 acggagccac ggaacacaag agaacacaga aaactgcaga ttttctcagt gggcctcagt
     9361 gacctccatg cctaagtggt ttctttcact tttgccactc ggatttatat aatacaccca
     9421 atcagttatt ttcggttatc tgattaattt ctataaatca gcctttattt ctttattaaa
     9481 aaggaaatat gaatgttttt gagagatact tctctttggc cggaaatcgc tttaagagca
     9541 atgattttat cggaattatc agccatgggg ctgaattcat aatcgaaatg gcctgactga
     9601 tattggttta agttgttgtc ggcaaaatgg atcaacggcg ggttataatt gtacagaact
     9661 ttccggttag cggtatccag gatggcaagc atcagtcttg tcctgtcgtc cttgttaaaa
     9721 tcgttgctga actttacctg tatgatgctg accctgttaa aattaccgat gttgttgctg
     9781 gaaagcagta acaaagtatc agtcttatcc ggcaagatgt cctttttttc aatgttcagt
     9841 tgaaaagccg tgtcatattg cctgtaagag taagtcttct tccagagcag gcctttgtat
     9901 tcctcgtcct gtttcaggaa tactttccag tatttttccc tgtccatttc cgaccagtgt
     9961 aatatgaact ccttgtactg ttttgtctgg ataaggttta caggaatggt gcacaatgaa
    10021 aggatgatga gagggatttt tattcctggc cgcagatggt ccataaacag ggcaaacagg
    10081 ataaagaaga ccggataata atcgatgaaa gccctcaggc caaagctgcc cccatagtac
    10141 cagcaccacc aggaagagat gaaataggta atggcggaga aaaaaaacag ccaggttaat
    10201 gccaggtaat atctgccgtc aacggccaac ctgattaatc cgaacaagca aagaaacaaa
    10261 acgggcgtat agacgaacag ccctttccgg taactgaaaa gagtattgaa gatttccggt
    10321 gatgaaaagt tgaagccttc atgctgatat gaatagagta aaaaggttcc tgtctgaaga
    10381 taccagacga tgcactgtat gcttagcagc gctgcaaaaa taagggagcc gcctatcagg
    10441 gcggggggat tttttacgat acggaatatc cctgatttca gctcattgac agaacctgcc
    10501 agaaacggca cgaaaagtac gataagggca ttaaaaggcc gtatcaggat aacgagcgcc
    10561 agaaaagcag aagccggaag aaaattcaag ggtttaccag tggtaaaata ccgtttggta
    10621 aaatagatga atgcgctgat ggcgaataag gaatagatat ggctgaaaga agcttcaaaa
    10681 cctgcataat caggcacacc ggtggcaaat gtcagcagga tttgcgacag gatgataatc
    10741 aggggtttaa tgttgtataa tttcagtatt tgtttcaaaa accagaggga taaaaacaga
    10801 tagaaaatgg ctgcatggaa aaccgatatc tggaaaggct tgctgtattg gtccacctgg
    10861 ccattgttgc gatgcaattc atgcatgtaa gtccaggcaa agaaaggcga gatcagcagg
    10921 gaagttccta cggagtattt attgaccagc cggctcttaa aaggaacata aaccggcatt
    10981 ttgtttaccc tttgatatac cggggaattc cccacccggc tgaagtcttt cctgaccagg
    11041 tctttgtgaa tgaaaacaga aggcaagtaa tcataatacc cttttccatc cgctttgatc
    11101 gttacatcgc tgttgccgat atagttattg taagtaagga tcaggataaa aatgattgag
    11161 agttctacgg cgattgattt gaaaattttg agcaggatca tggtttaaat ggttagcagc
    11221 ggtggaatgt gcagtaatag ttaaaattta cgataaagat gagaattttc ttgctattac
    11281 tcaaacagca ttgttgggtc agcaacaaat ttgtggcgtt ttttttgccg gaggtgcaga
    11341 ttataacgca gaatggcttt aagatgttaa aattcaatat gataaaaatc gcaacgggtg
    11401 ttctaaaatt aagatgtacg tctgaaaaag atcttcattt ttgacggctg cacgtgtcat
    11461 ggttctattt cagcttggtt ttctgctgag cagacacata attaaacatc agttgtttag
    11521 aggcttgcga taataaccat tttcttctgc gacgaaaaac cctgtatcgg aggatcaggt
    11581 actccttcgc cgaaggtatc cggtaaaggg ttccgtaatt ctccaaccga caaggcctga
    11641 agtcatcggt ttcatatctt ttcagcgctt ccgccatcat ctcaggcgtt tcggagtata
    11701 acctttcgcg caggatattg taggtgtcgg taggatggat gggaataatt ttctcaacaa
    11761 tcggcattcc ggtgtcaatc cattcgttaa tccgctgaac cgttgctccc atttccgctt
    11821 caccgttata caattcccag aaagcggccg gctgtccgcg gtattcacgc atattgccat
    11881 ggtgatagga aagtacaccc aggggagcaa tctcgagcaa gggtttcctg atgatagtgc
    11941 cgaagccgct caggtaaacc agaatatcaa ttttttccga tctgatggaa tccagggttc
    12001 tttccgaata aggatcatcg gttttaaaaa caggaatatt atagcggctg caaaaatctt
    12061 ccgcagacag ggctggttgt tttgccctga aaagattgcc cgccgacata aagaaaatgt
    12121 atgatccttg tctctttttt atgtggaaaa ctgatttctt caggaatgat tttccgggcc
    12181 tgacgtcgat gtagcagcag gcaagcttgt cattgtgcaa attcacgatg tgttccagca
    12241 ccttatggtt gaagctgttc aaatttgacg aacaaaggat gccgactttc atgaagacct
    12301 gtattgaatt ggcgccaaaa atataaattc gccatgatat aatcgccttg aagactaaaa
    12361 gcgatttgca gaatctcttt cttttgatag cgtgcgatgc accgggaatc gtcaaccggt
    12421 gcagccggga gcaggccggc ggagcgcccg aaggatgacc ggggggcaac cggaagggga
    12481 aaccggcctg taattgccgt tttttttgct ccggctgcca agccaggcct tatatgcaga
    12541 tttccattcg cgtcgctcat tgtataaaat ttgtaatatt gcgaaaacgg tcgaaacggt
    12601 gttattccgc cgggacattt tacttttacc ttatgttatc atcatcagac ttaaataatc
    12661 catggcgtaa agttgcttcg gctatttaca gaaaaccggt tgactccaaa attttcggtt
    12721 cggtagagat ggacatcacc aggttggagg aatacatcag gatgaaacgg aaacaaggcc
    12781 tcaggataac cctgacccat gtttttaccc ttgcggttgc cagggctatc agggaggaag
    12841 tgccggagct gaattcatat atccgacgcg ggagaataaa gcagcgcggt catgtggatg
    12901 ctatggtaag cgtgctgctg aaaggattac agatgagcac gatcaaagtt ccggatgcgg
    12961 acaggcttac cctgggggaa atggcgctgc tgctgcgcaa cgaaatagga aattcgagga
    13021 aaggcaacga aagtaaaacc atgaaactaa agaggctcat ggctgcaatt ccctggccct
    13081 tccgtaactg gttatacagg ttgttgaaaa aaataaccat agactggggt ttttcgttgc
    13141 catcagtggg gttaggggtg gatagtttcg gatcgtttgt aatgtccaac attggcagta
    13201 tcgggctcga tatggggtat cccgctttgt tccctgtatc gaatgtatca atggttttta
    13261 tcctgggtgg cgtcagcgat aaagcatggg tcgtggacgg acaggttgag gtcaggagaa
    13321 ttatttccct cggagccgcc ctcgaccacc gtgctgttga tgccagtcat ggagggaggc
    13381 tgttcaatta cctgaaatat attgccaaac atcctgaaac gctggaagaa aaaccttcga
    13441 tcatctgacc aggagtttga aattttttgt ttacctgaga tttatttcgt aaattaacat
    13501 tcaaaatctg gttacgctgc tatgactaaa tacctgcgat atgtgtggat ggctgccatc
    13561 gttgcagcaa tggcaattat tgcaggcagg catttcccga attatgccct gcgcatttct
    13621 ttcttttctg ctttcctttt cattgatgca ttatattggc cagccttccg gaaaacttta
    13681 agccccggta aagcgttagc tgccaggtgt ttgctgattt attactggtt gccgttcgtt
    13741 ttactggtgg cgacagcttt ggccgcagtc tttatacccg ttcaaaaatg gttcccgttt
    13801 tttcgtattt atattcccgg cttcattgtt gtgctctata tcgggaaaat attgctcctt
    13861 attattttga tgctgagtga tttattgact gcgccgtata acctgattgt ctggttccgt
    13921 tcttccggga acaaggccgg ctatgaatgg aaaagattta aagctgtggc ttatacgggt
    13981 ttggccgtat cttttaccgt aatcctgatt ttcatggcag gaatggttca ctgggtttat
    14041 gatttcaagg tgcagaatgt tatggttgaa atggatgagt tgccggcatc ctttaacgga
    14101 tttaaaatca tacagatatc cgacgttcac cttggcggtt ggcggaccaa agaacgcctc
    14161 cgtgaagctg tatcaatcat cagacaactg aaaccggata tggtggtttt taccggtgat
    14221 atggtgaatt tttcatctga ggaagcgctg ccgttcacga aagagttgag gcggctcaaa
    14281 gccccttcgg gcgtatatgc catcaaaggg aaccatgatt acggggatta tctttcgtgg
    14341 gatacgcctt ccggtaagga taagaatgtc cgtgaccttg atgctttcta taaaaatatt
    14401 ggctggcagt tgttgaataa tgcccatagg cttctgatca ggggaaatga taccatcgtg
    14461 ctggttggtg ttgaaaactg gagcgcttcc cggctttggg gcaaacgcgg gaatctgaaa
    14521 aaagcgcttt tgggagctga acatcccggt tttaaaattt tgctgtcgca tgatccgact
    14581 cattgggaca acgaggtttc cggattgtat cccgatatcg acctgaccct ttcgggccac
    14641 acccatgcca tgcagatggg ctgggaaggc agaaacagcc aatggagccc ggcgcaatgg
    14701 aaatacaatg aatgggccgg attgtacgag catgactttc cggtaaaaca gtttctctat
    14761 gtaaacccgg gcctgggaca tatcctgtat cccggaagga taggcatcag gccggagata
    14821 acggtgattg agttacggca ttaatccgga aggccatcaa cgtcggcagt actgaaaatc
    14881 aaaaccagga accgataatc ttgtttttcc ggtcattatc atgcttgtgc ctgaattcat
    14941 tggcatgctt atcatagctg taatctttgc cccattcttc atggtgaagc gaaatctgac
    15001 ggatggcttc aaggcagcag tccagttcat catcggtcat ggtagggtgg agtgaaagcc
    15061 tgatccatcc tggttttgat gagagatcgc ccatgctgat gaatttggat atttgtgctg
    15121 atttttcgaa ggtaacatcc agaagataat gaccgtaggt tccggcgcag gcgcagccgc
    15181 cacgcgcctg tatcccgaaa cggtcgctca gcagttttac caccaggttg taatgtgcat
    15241 gatcgaggca gaacgacagg atgccgagac gatcttccac gttatccgcc aggatgtgta
    15301 tcccgggtat tttccttaat tctttgaaag ccttttctac cagttctttt tccctttttc
    15361 gcatgttttc aacgcccatc tcatttttca ggccgatgca aagggctgtt ttcatggctt
    15421 gcagaaaacc cggggtgcct ccgtcttcac gaatttcgat atcatcgatg taccggtatc
    15481 cgccccaggg gttggtccag ttcacagtgc cgccaccggg ttggtcggga gtgccctgat
    15541 gatataattg tttattgaaa atcaatacac ctgaagctcc ggggccgccc aggaatttat
    15601 gcggggaaaa gaaaatcgca tcgaggcact ccaaaggatc ggaagggtgc atgttaatgt
    15661 tcacataggg cgctgaggct gcgaaatcaa caaagcatac tccgccggct tcatgcatga
    15721 tcttagccag ttcatggtaa ggcgtttcga taccggtaac attggaacag gcagaaaaag
    15781 atcctatcag tttttttctt cctttatact tttccagttc ggtacggagc atctccggat
    15841 ccaccagcag cccattgccc ggcggaataa caaccacatc ggcgatggtt tcgtaccagg
    15901 atgtctggtt gctgtgatgt tccatgtggg tgatgaagac aaccggcttt tccgatttgt
    15961 ttaggcattc ggagtgctgc aggattccgc aggtcttcag cccgagtatg cgctgaaatt
    16021 tcaccagcac gccggtcatt ccaaatccgt ttgtaatgat aacatcctgg gggccggcat
    16081 tcacatgctt ttttatgaga ctgtgggcat agtgataagc ctgcgtcatg atgctgccgg
    16141 cttcactggt ttcggtgtgc gtattggcca cccacgggcc tatttcatgg agcagttttt
    16201 cttcaatagg ccggtacagc ctcccgctgg caatccagtc ggcgtaaagc aattttttag
    16261 ttccgtaagg cgtctcgtat tccaggtggt tgcctacaat accttcacgg aaacgggaga
    16321 agtaatcgga aaggttatcc atttttcagg cttgcatagt ttcctgcaaa ggtcgtgaaa
    16381 aattggcgaa atactgtttc cgaaaagttc aggcagatgg ctattcatcc ccctgtgaga
    16441 tctgtatccg gctgatttca acatccgaac cttcggtgta tatgctgatc cagttgttgt
    16501 cctcacggta ccagcggtct tgtccggcaa gacgcacaag gtaatcggcg ccttcgccgg
    16561 gagagatggt gcgtaacact attccgccgt ttttctgggc atcgcgcccg taattgagga
    16621 aaactttcgg aatattgcct gaagtggccc tggcatggat caggacataa ttgtttttcc
    16681 gcagcctggg ttcaatcacg gcaaatgaga atttcttttc ttcacccgct gagatggtca
    16741 cagcgccggt atttacctcc ttgatggcat gatcggtgat gtattgtttt atcttgcgga
    16801 tcatctcacc gggataggtt tcgtcaggtt taatggcgga tgctttttcg taagattcaa
    16861 tagcctggtc aagcaccttt tgccggtaaa agttatcggc gaccacaatc gctttatcgt
    16921 attcttcttt tagcgctttg gcctgttcct gtaatatttt attaatttcg gccagtttgt
    16981 ctttcggata ttgttccgcg ggctttagtc ccgaagcttt gttataagcg ctgcgggctt
    17041 ccgggtattc cttccctgcc aaaaggcggt cgccctcggt gataaatccg gcgtacatcg
    17101 tttccagttg ctctttttcc agggcaagcg ccttgtttat ttcgtcaatt ttttcccggg
    17161 gatacttttc ttccggtttg atttcacttg cctgctgata ggcattcaaa gcctgcgggt
    17221 attccctggc tgccaggcgg gcgtctcctt ctgctatgtt cttgttgtat tggttgtcgg
    17281 tttctttcaa ccgggcttca atatcgtcga tcttttgcgc gggatatgtt tcgccggact
    17341 ttatggtaag ggcattgcga taggaagtca aagccgcctg gtaatcattt gaagtgtaat
    17401 gggcgtcgcc ttcggcaagg gctttgttgt atgcttcatc ccgtgcctga atttctgcca
    17461 gcagcccgtt gatagcgtcg attttatctt tcggatactg ttcttctggt tttatttcca
    17521 gcgccccgcg gtaaccggaa agcgcctggt ggtattgctt ttcggcaaag gctttgtcag
    17581 ccccgacgag gagtgttgtg taatcatcat cccgcttttt ctgttccgca agcagttggt
    17641 ttatcttgtc gatctgctgt tttgggtagg tttcggcagg ttttatggta gctgcacttt
    17701 cataaggctc gagcgcttcg cggtacttct tctggttata gaaggcgtca gcattggcaa
    17761 tggcggtatt gtatttgtcc tcaatggctt tgatgccgtc aaggatggtg ttgatttcag
    17821 cgatcttctg cgtgggatac ttttctgcgg gaaggatgct gatagcctca ttataggcgg
    17881 ccagcgcccc ggtgtatgct tttgacgcca gttgcctgtc tccttcagcc acggcttttt
    17941 catacgattc ctgcctggct ttcagatcgg ctattttttt tccggcttcg gtaatcttat
    18001 cggccggata cttttcgccg ggcttaatgg taagggcgtt gttgtaaccg gcgatcgact
    18061 cttcgtattt ttcggatccg aagtacctgt cagcttctgt gattgccttt tcgtaagcgt
    18121 cgttcacggc ttgtatacct gccaggatgc tgttgatttc ggtgattttc tccgccggat
    18181 atgtttctcc cggtttgatg cccagggctg aattatatcc ggcaagggct tgcccgtatt
    18241 gtttttgggc cagttttgaa tcagcatcgg caatagccac ggcgtatgca tcatcggtgg
    18301 cttttatccc gttcagaatg gtattgatct cagaaatttt gttggccgga tacgattctt
    18361 ccggtttctg aacaagcgcc tgccggtacg aagctatcgc ttcttcgtag tcctttgcgg
    18421 caaagagttt atcgccccgt gttacagctt cttcataagc aacctgcttt gctgcaaggt
    18481 cgtcaaggat ggctttaatt tccgtgattt tatcaagggg gtattgctcc gagggcttta
    18541 tggtggatgc tttctgaaac ccggccagcg catcgggata ctttttgtcg ctgaacgatt
    18601 tttcggctga tgtcagcgcc gactggtaat tatcttccag ggctttgttt ctggcaagag
    18661 cgctctcagc ttcggcgatt ttgtccctgg ggtatttttc agcaggttta atctgcaaag
    18721 cttcattata agccccgata gcttctgtat attttgaatc agccagcaac tggtcagccc
    18781 gggagattac cgaaacatag ttttcgtctt tcaactttat accggccagg atgctgtcga
    18841 tctcttttat ttttccagcc ggatattctt cggccggttt aaggtccagg gctttcctgt
    18901 aaccggtaag ggcttcggta tattttgcgg cagccaggga tttgtcggcg gcagcaacag
    18961 cgctttgata agtgttatca agttgtttct gctcctgcaa tatccggttg atttcggcca
    19021 tcttatcttt cggatatttt tcggctggtt ttagcgcttc tgcctgcctg aatgagtcca
    19081 gggcattgtt atagtccttc tcggcaaaca gcctgtcgcc ggcggcaacg gcattcttat
    19141 actgatcttc tttgtcctga cgggctttgt agatggcatc aatctcattc agttggtcct
    19201 ggggatattt ttctcccggt ttgagcgcca atgccgattg ataaccggtg gcagcttcct
    19261 cgtatttctt ctccctgaat ttcagatcag ccgttgcaat ggccgtttta tatgattcat
    19321 ccgctttgtt tcgttccaac agcaaggcat ttatttcggc gattttatcg gcaggatatt
    19381 tttcatcggg caacagggta agggcatttt tataatgagc cagggcagct tcaggcttgt
    19441 tttgctgaaa cgcctgatca ccattcatga tagcgatgtc ataagcttct ttgcggcttg
    19501 ttttttcacc tatcgacttc tcgatttcag cgatgcgcgt acggggatat gattcgcccg
    19561 ggagaattcc cgatgccgat ctgtatccaa gcattgcggc atccatgtcg ccggatttat
    19621 agaaattgtc ggccgaggca atggctgcat cataacgttg ctgtaacgat ttaacttccg
    19681 cttcgcccgt gctcgtttta tcgagcatgt ctttcggata acgggcgttc ggttttacct
    19741 taagcgcttc ctgatacttc acccgggcat tggtaaaatc gcggctgatg taaaactggt
    19801 cagcctggct aatggcgcgg tcataagctt cctgctcttt tttccgttca ttgagccagg
    19861 cgtcaatttc ggctactcgc tgaacggcgt aggtctgatt tggcaataag tccagagctt
    19921 tctggtactc ggcccgggct tcggcccgtt tttcagcctg gatgagttta tcggcagcgt
    19981 taacaaattt atcatactgc gatttcttaa tggcagcttc cgctttcagt ttatccgctt
    20041 ccagtatctt ttcacggggc tggcgcgcat ccggatccac ggtaagagca atctggtacc
    20101 agttttttgc ctggtcgtaa tcggctgcaa ccatggcctt atcggctttt agcatggcgt
    20161 cattatagcg ggcggcatcg cccggatcgg tgtacacctg ccgtatcaat gccagcttgt
    20221 ctttgggata ctgtgcttcc ggatcaattt cgagcgcttt tctgaattcg cgttgagccg
    20281 aaggataatc tttgttctgg taaaaatttt cacctttaag gatcgcatcc tcgaaaaggg
    20341 agttttttag gtccttattt tctttgagaa agtccgatat caggccgatt cgctcctgcg
    20401 gatatttctg ggcaggatta aggatgccgg catttcggta atgcatcaag gcattgccat
    20461 aatctttggt attataacaa tcgtcaccgg ctttgacttc ggctgcatag gcgccgtttc
    20521 cggtaccctg cgaagcctgg gcaaaggtat attcagggct ggcgctgaga aaaagcgcca
    20581 gacagacaaa aagggcagta tcggaaaatt tcagaaggga cagtatattg aaatgcgcca
    20641 tgtttagttc ttttggtggg attatccgtt tcgcatgaaa aataacgggg gaaaggctga
    20701 tttattggac gtgcaacgat cctgacgccg gagtaaccgg acagaagttt cggcgctata
    20761 cgatcaatcc gtccgtaatg gtgagcttgc gatccgacat atcggcaaat tccttgttat
    20821 gggttacaat gacaaaggtt tggttgaatt ctttccgaag gtcaaagaaa aggctgtgca
    20881 attcgcgggc attcaccgaa tcgagattcc ccgaaggttc atcggccagt acaatatcag
    20941 ggtggttcat cagggccctg gctactgcga tacgttgctg ttcgcctccc gaaagttcgg
    21001 aaggcttgtg atttgccctt tcggtgaggt gaaggaaatc cagcagttct gttgcacggt
    21061 tttcggcttg tttccgggat gaaccgctga taaaggacgg aatgcaaagg ttttcgatgg
    21121 ccgtgaactc cgggagcaaa tgatgaaact ggaagacgaa accaatgtgc ttattgcgaa
    21181 acgccgacaa tttcttttcg ttaagcttgt ggacggcctc gccattcagg acgacacttc
    21241 cggcggtagg ccggtcgagg gttccgagaa tctgcagcag ggtggtcttg ccggcgcccg
    21301 atggccccac aatcgtgaca atttcccctt tatttatttc gaggtctatt ccttttagta
    21361 cttcgaggct tccaaacgat ttatgtatat tactggcagt tatcatttta tattatattg
    21421 ggcgtcagtt tttaaatggc ttataccgtt gtcaacggct tcttttcagt tgctgatagt
    21481 cgaggtagta caggatttta aacgatacat tattttgctg gggagaatca aaagtatcgc
    21541 tgaaattact gaaaaagttc tctgctgctt tgcttttttc cgtcaggatt gaatttttcc
    21601 agacaatgtt catgctgctt cccggcagga aaagccagga aaacaccatg tcgacattaa
    21661 acgcattata actgaagtta ttatcctttg tgtatccggc attttcctcc agattgccgt
    21721 tttgaaggag tgaatagtac tggtcgtatt ttccggttga ccagtaatgc cgggccgaca
    21781 aatttaagga cagattgttg ataaacagat atttacagtt caatacattt gcaacagtcc
    21841 tgacatcccg gttcccgaaa ataatatcgc cgtcatcccg ggtatcggca tatccatgtt
    21901 cgttgaagac gctctcaagc tcaaattcat atttgagcat gaagtggtta ttcacgcgga
    21961 cgataggccc ggcattgact ttatagccat gggttttatc ccggctggcc gagcgtaagg
    22021 taactccggc gtcaagggca aaaggcttac ggtaatcact tgatattcca agagttccgc
    22081 ttaatgcttc tggttgtata tagtaccggt tatcggcgcg ggtttcataa tagtcgtgac
    22141 cgtttgggaa ggtataattc atattggccc ataccgtgag ataattcaac agcgtagcaa
    22201 cgctctccag ttcgagttct gtttcctgca tcaggccggt agtatggttg ttggtgtttt
    22261 caaggctcag ttcattatgc cagtcacgga atatccacca tggctgatag aggttatagc
    22321 tgacatccgc ttcgtttatc gaatagttgt tatataaggt tactccgagg tcattggcgt
    22381 taaaattctt atccatgatg ccgtggagga ttaaccattg aaaacggcca ttcacttttg
    22441 aaagcccggc gaaatatttg tatccgaggg taagattgct tttgcttccg gatgaatcgg
    22501 gctggttata taactggctg atgccgccgt tgagattgaa ctggtaaatg ttttttttgt
    22561 tattcaactg aatgcctgct gcagtaacgt tggcatcgcc gaagcctttt ccgcggataa
    22621 cgttggtatt gataaggtat atatcggaat tgtttttaag ggcctggtca aatacaacaa
    22681 tactgtaatt tgtggcagga tcggtaagta ttttcttttc cttgcccgaa atcgtatcct
    22741 cggcaattgc ataggtattt cctgtaacgg cattcaggat gcccagggca aacccttttt
    22801 tacctcttcc cgaaatttta gttgcattca gcagtcgctg ctggaccgga ttttttttga
    22861 gcttataccc ttcgccggcc atatcttcag cctcgtaaaa atacatcggc gttttaccga
    22921 tgcggcggga gtagaaaatg tcgcctttat tgtacaggtc gacggcttcg cggaaaaacg
    22981 gacgttgttc cgtatatacg gtctcaaatg cagtgaggtt ctttacttta ttgtccgatt
    23041 gcacctgact gaagtcgggg aggagcgtca tgtcaacggt gtaactttcg ttgatgccgt
    23101 atttcaggtc ggttccgccg ccgaaggacg ttgaaatgtc ttttttttcg gtttgaggga
    23161 aatgttcaaa acccacagat acatatggcg tgagcgataa ccgcacaggc ggtttaacat
    23221 cgctgatgcc ggtaagatta ccccagtaaa cgaggttatt cccggctccg ggcttttcgg
    23281 gtgaaaactg gtcggtttca cggtaacggc gcacggtcct tattatctga aatccccagc
    23341 attgagtttc ggttctggga aaccggatgg ccgaataggg aatcatcagt tcggcaaccc
    23401 accctttctc tgttttcctg acggcgctca gccatacgcc gttataggtt tcgtcagatt
    23461 tccttgcatc gagctggact cccgacgatg ttaccgtgaa ggaataggca tcaagctggt
    23521 tgttataggt gtcgaattca acttcgaaca tgtcggcatt caggttatcg tcatcgcggt
    23581 tgccaagttg catcagaatg ctgtcgggat ggggatcatg catgagggcc cccacataaa
    23641 tggcataatc atcatataaa atccgcactt cggtgggaaa tgagggttca tggccgaata
    23701 acgggagcga ttgtttaaaa tcaccggtta ccggcgcttt aatccatgcg gtatcatcaa
    23761 gtattccgtt aatgcgcggg gcgttcagca ccctcacagc cacggctgtt tttcgggatg
    23821 tcaggttgct gactgcgttt tgtgattgta aggttaaggt aatcagtaat aaaagtacca
    23881 gtgagaagga ctgtcgcaca aaaatggaat atttaaggtt tcaacagcaa agatatgatt
    23941 tatatacatg tttgaaagtc cctttaaatt gactatattt aatattttaa attcatgaca
    24001 tatgaacctt catgaatatc aggcaaaaca gatattaagg tcattcggcg ttccggttgc
    24061 tgatggaatt gtagcttcaa cagttgacga ggccgtggaa gcggcagccg gcattcagca
    24121 gatgacagga tcccgatttt gggtggtaaa agcgcagatt catgccggcg gaaggggaaa
    24181 aggcggcggg gtaaaattgg cgcgaaacct tgaggaagtt cgtgaaagag cttcagaaat
    24241 tctgggaatg aggcttataa ccccgcaaac cgggccgcag ggaaaattag tccggaaagt
    24301 ttttattcaa caggatgttt tttatccggg gccttctgaa ccgaaggaat attatataag
    24361 ccttctgtta aaccgttccg aaagcaaggt catggtgatg tattcccccg aaggaggaac
    24421 ggatattgag gaggtggccg aaacaaaacc ggataagata tttgttgaaa tcattgaccg
    24481 ttcagcagga ttacagcctt ttcagggcag gaatatcgct tttaacctgg gattatcggg
    24541 cgaggcgttg aagaatttgg tcagatttac cgatggcctt tacaggacat acatgggcgc
    24601 cgatttaacg ctgctggaaa taaatcctgt acttaaaacc tctgatgata agataattgc
    24661 ggtggattgt aaaatgcgcc ttgacgggaa tgcattgttc cgtcatcctg attatgccgt
    24721 gatgcgggat atatttgaag aggatcctgc cgaggtggag gcaggggaat ataacctgaa
    24781 ttacatcaag ctaaatggca atgtagggtg tatggtaaac ggcgccgggc tggctatggc
    24841 taccatggat atcatcagac aaagcggagg tcagccggcc aacttcctgg atgttggggg
    24901 gactgctaat gccgagcggg ttgaaaaagc gttcaggatt attctgaaag acaggaacgt
    24961 tcaggcaata ctcgtgaaca tttttggggg catagtccgt tgtgaccgcg tagcgcaggg
    25021 aattgtcgat gctttcagca atatcggcga tgtggatata ccggtaataa tccgcctgca
    25081 ggggaccaat gctatggaag ccaaagaact tattgataaa tcgggattgg cggtatactc
    25141 tgcagtgacc atgcaggagg ctgcggacat ggtcaacagg gtgatcaaag ccagggcgta
    25201 acaggcgggc ttttttaact ggcaatgtca gtaagaataa cgatgcagat aatacaaaca
    25261 gttttttcag ggcatttctc aaaaatttcc ttagtaaaaa cattttgaaa atctgtctga
    25321 tcatccacta agagaaaaat tacgcttaat gcaagcgatg atcggggttt tagacaaatt
    25381 ggcgctgtta tttactggtc ccgacaagct aaaaaaaaat ataaagaaat acttacatgg
    25441 taaattaaat tataaaatca tcgtatttag ggtcaaaata ttaaaaaaat ggcctgaact
    25501 tcaaaaaaaa aatttgcttt cgaaaagact gtaactggct aatatataac atgttaagtg
    25561 tatagttatc catcaattaa atattttttc ctgatgtaat tggacaaaag tattcttttc
    25621 gctatggaat ttttatattt tagcatcatc gaaccttaca taatggtatt aactacctaa
    25681 actaaaacaa acaagctaac cctaagttta acattaattg taagtgctta tgaaaaaagt
    25741 aactttacta ctagcgctgt ttgcattttg ctcatggcag tttgcgctgg cgcagaaaac
    25801 aatcaccgga aaggtcaccg atgccaaaga tggccttact atccccggtg taaacgtagt
    25861 ggtgaaagga accactacag gaaccactac cggcagcaat ggagagtatt cattaaaagt
    25921 ggcggctaat gcacagacgt taatattctc ttttgtcgga tatgtaaccc aggaagttgc
    25981 cattaacggt aagaccacca ttgacgttca gctcgctcca acggcagaac aactccagga
    26041 agttgtggtt acagcccttg gtatcactag ggaaagaaaa tcactcggat atgcagtgac
    26101 tgaggtgaag gctgatgaat tgtcgaaagt atcacccata agtcctgtta acgcattaca
    26161 ggggaaagtc gccggtttga atattgctcc caatgaagga ggggtattcg ccggagcaag
    26221 aattcaactt cgtggggcct ctacattaaa agagaataat atgcccatat ttgttgttga
    26281 tggcgttatc gtcgataacg aattatcagg aggaagtgaa tggggaggtg ttaactgggg
    26341 gaataacctg aaaaatctga actcggatga gttcgagtca gtgtcagtac tgaaaggatc
    26401 agccgcgact gctttgtacg gttcacgggc attgcatggc gctatcgtca tcacaacgaa
    26461 aaaagggaaa acagagaaag gcatcggcgt tacattgtct cagaggttga attttaagaa
    26521 agcctatgat gggcctgctt tccagaatgt ttatggagaa ggcgcaattc ccggatacga
    26581 cgatcgctat gaagatatgt ttgcccctca gaattcgttt ctgatcaatt cggctggaga
    26641 accttatatt gaaggtatat ccggtggctg gtgggtgcct attagttacg gacataagat
    26701 ggatggcacc aagattcgtg attatgaagg caactggatc aactatgacc cgcagcccga
    26761 taactacctc cagactttcc agaatggttt ccagtcgaat accaatgtga ccatcgacgg
    26821 gagcggggat aattcttcct tcctgatttc atactccaat atgaaagata aaggggtgta
    26881 tccaaggaat acttttgacc gtaactccat tttctccaag atatcaagga agtttaacaa
    26941 gtatctctcc tccgaacttg ggataagcta ttcaaattct gtttcaaaga atcctccgaa
    27001 atatgttgaa cagaacttta ttaccggtaa ttggccaagg aattataata ccatctatgc
    27061 acaagatcat tacaaagcag ctcatggcgg taccccccag gagcagtatg gagatccggg
    27121 atgggctact ccgggagctg atatctggtt tgatgtattt gaagatactt atgcacaaaa
    27181 tgaggaagcc cttcgtctta cacctaaggt taccgttaac gtagccgact ggtttaatgt
    27241 tgtttttgaa ggttacctta ataattacta taccaaaaca gagacgaaag aactgggtca
    27301 gggatatgct aatgaaggtg gatattattc attgggccac agtacaaagc aacaacttga
    27361 cggaaaactc tggctaaatt tcaataagaa aatcacagaa gattttgatt tccgtggatc
    27421 agttgttgct gaacactggc agacgcatta taactattca aacgcctgga caaatggcgg
    27481 cttgattgtg ccggggcaat attcattatc gaacagtaaa ttggaagctg gccaggaagc
    27541 cggatacaag gatcaaaaaa tattgcagtc attgtatgcc tttgccgatc tttcatggaa
    27601 aggccagtta taccttactg taaccggacg taacgactgg tcctctactc ttgtatatgc
    27661 cgacagaaca ggcaattaca gctatttcta tccttcagta agccttagct gggtgatgac
    27721 tgaaagttta gacctgccgt cctggatcag cttcggcaaa ctcagggcat cctgggctca
    27781 cgtgggtaat gactttgatc cttatgagat caacccgggg ttctccagaa tcggaaccgt
    27841 gaacagctat aatggcgacc ttccaatgta tagtttcaag aatgatgtaa tgcctaacct
    27901 gaacatcaaa cctgaagata aaaaatccat cgaattaggg gcagaatatc gtttcctgaa
    27961 caatcgtttt gggttcgact tcacttacta taaggaaaat acgttcaacc aaatcctcaa
    28021 aatcccggca ccgattgaat ccggagtaaa ttatatcctg ataaatgccg gtaatatcca
    28081 aaaccagggg gttgaactag aattgcatac cacgccctta aaaatcaatg attttgaatg
    28141 gtttgtagat ggtactcttg ccaggaaccg tagcaagatc atagatctct atgaaggagt
    28201 tgatgagtat tcccttgaag gatcgtggaa ttatggtaat acacgtattg catctatggc
    28261 aattgttggg gatgaatatg gtgtacttgt atctgattca gatcctcttc tctataataa
    28321 cgaagatgat cccaatgacc cgctaaatgg tttaccgatt ctttcttgga gtaattcgac
    28381 ccgtggtgtt tatgaaacaa gaagttatga gaaaaaagtc gttggaaata tgaatccaga
    28441 tttcaccgga agtgttacta ccggtttctc gtacaaaaat ttcaggatat ctgccatgtt
    28501 tgatttcagt tttggtggtg acatcagcac ttattcagga cgttacggaa cagcttatgg
    28561 cttgttcgag tcgacactgg ataaacgtga tgcccaaagc ggaggtttag tatggacaag
    28621 tccttggaca gggaaccaat atgaagacgg aataattcct gaaggcgtat ttaaagatgg
    28681 aacaatcgtg gatatgaaag atgcagaagg caatgttgtt cataacgatg taagcggaat
    28741 gacttataaa gaagcttacg aacaaggtat gatagaacct acccatgttg agtactggta
    28801 ttatcagacg aacagttggg gccgtggcgt tatcaatgac aatgttttac aggagaattc
    28861 ctacattggt ttccgtgaat tgtcacttac ctaccagatt ccgtcatcca tccttgaaca
    28921 tataaaaata tcaagggctg atatttcttt cgttggccga gatctcggat atctttataa
    28981 aacgcttaag gataacctga acccgttcag tgtaagaagt aaccgttccg gcgatgctca
    29041 tgaatggcag caaactcctt atgtgcgttc cttgggggtt aatctgaacg tttcttttta
    29101 acaggaatcg atgtctaatc ttttaacaaa aggaagttat gaaaaatgta tataaaaaac
    29161 ttggattagc attaatggcc atcagcttgc tcattgcacc gcaatgtaag aaagagtttg
    29221 ccgacattaa taaaaatccg gatgaaatca cagatcccga tcttacgcat ctgttcacgg
    29281 atgccattta taaaatggct ggagatgaat acctccaatg gttctataac aacagcgttt
    29341 atttttggcg cttctctcaa atgacagttg cccgtggggg aaccagctct gattttaata
    29401 atactgcggc tctgggaggt gttcccttct acaccgtcat gatagacatg aaggaaatca
    29461 gggagaggat tgataatatg aatgatgaag aaaaagccaa acatcaggca ttgagggcgc
    29521 taacctacat tccccaggtg tatttgggaa ttaagtacac cgactggtat ggaagtacgc
    29581 cttacaccga agcaattgaa gctagatata cgggtaattt taaaccagta tatgattcgc
    29641 aggaagaact gttcaatgtc tggctgtccg aactaagttc atcaatagat gtattattgg
    29701 cagcagataa cagtcagtta tcacctttga atcaggattt tatttttcag ggtgattggg
    29761 ttgcatgggc ccgttttgcc aacagtctga ggcttagaat tgctgcgcga atggagcatg
    29821 cggatcctgc actgatgaag gaaattctcg gagaaatagt cagcaaaaaa gtagatggag
    29881 aacttgcact gattataaat aaagaccaac aggcaatttg ggctcctgga gcttctgaat
    29941 tagggcctgg gggaacaaat tctttgtgga ttgagaacta cggaccggca cagaatttca
    30001 gtaagtatat gtgcagcaat aaagatccgc gaatgcaaat atttttccgg aaaaacggct
    30061 tgtctgatga ggctattaca ttacttcagg cttcaggcgt cacagtacct tcatttgcca
    30121 ctttaccggt aaatgatcct tgggatcgtc ttgttggtgc acctgttgca ccggatgatg
    30181 ctggtaatct gaattatttt ggatccacgc ttgtagattc caatggaacc caatattcaa
    30241 ggttgccatg ggttgaatat ctttatttca aaccaaaaca ggatggagga acaggcgaat
    30301 acatgaacta ccttttgggc gcacctgagg tatgcctcta ccttgcagaa tttatcgaaa
    30361 aaggatatgt tagcggaatt ggatcggctc aggaatggta cgaaaaaggg gtacgctatt
    30421 catgtgagaa ttacgatgtc cgtgccagca aagctcagat tgataattat caggatcgaa
    30481 aaattactcc ggaagcaatt gatgcattgc tcgctgccga tggagtgaaa tatggaactg
    30541 ataaccttga aaaaatcatc cttcaggaaa ttatcaatct gttcgacaat ccttatgaag
    30601 gtattgccgt aacccgtcgt acgggatatc cgaaacgttc gagtgatctg tgggcatggc
    30661 aaccttacct ctcttcaggt actgaattga aactgccccg ccgtttcccc tggggaactc
    30721 ccggagatgc aacaaacctg gccaactggc aggaagcatt gagcagccag ggatttactg
    30781 ctgatgtaaa taccggagat gtattgaatg cgcaacgtgt atggtgggat aaaaacagcc
    30841 ccaactatgg cgatggggac taattctcat tgctgaatga ttcggaaaag aggctgtcaa
    30901 ctggcagcct cttttcattt tatctaatag ctttatatgc aatctattac cgggattatg
    30961 attcccggaa tagaccatat actccgaagt aagaataata ttgagtatat gttcccgttc
    31021 aattattcct gattaaatcg gggttattca tgatgatgtt ttgccattaa ttgaataatt
    31081 atttgttagg actggtaaaa tttatataag tttgatattg aaatctattc agacaatgtg
    31141 atgatgaaaa gatacctgat tttttttgtg acatattttt gcctggcttt tatgcaggag
    31201 gctgttgctc agcgcgatga taaatccagc gccattctcg gcatgaccgg cggaatgggc
    31261 atcaccaggc tgcaatacaa cagctatgat aaatcgctgt accattttaa acaaaccgct
    31321 tccagctatt tcggcgtttc gcttgaattc cccgtcccca agacggaaaa ccgggggact
    31381 ttctacaacg agctggcttt tttcaggttc aaatcttccg catcattgca tactgccgac
    31441 agtgtaaacg gtaatccgga aaacagctat tatgacataa accttgaatt tgagcccaat
    31501 attatcagtc tggcccatat gttccgttat tcttttacgc ctggaaattt ccggtatttt
    31561 gtcagcgcag gtatttacaa caattttgtt atttctgccg tcaaccggaa aacaacggtt
    31621 catacgctga atggcgttgc aggggaacct taccatgaag atgccgtgac cgattttgca
    31681 gtccacgggc tgatgatcac ggcaggcacc ggttttatct acaaaaatat aggcctggaa
    31741 ttaaggttcg atccgggaag gaactttaca aaactttacg actgttcggt ttattcaatg
    31801 actttctcgg cattgctgca tgtaaggttt aatccctgaa aaagagaaat accagcctct
    31861 tgtccggccg gttccggatt aaaatcattt ataaatccgg agcaatttaa ccccgtgtga
    31921 cggaataacc gttgaatagc tatcacccaa agggccgatg tcttcctgtt tccagacgtc
    31981 gcggatggtg taattgccgt tcatgtccag atcgctgcgt tttataacgg cggattcctg
    32041 tttcatgccc ctgttgaaaa tgccgaccgc tttagaacca tcctccaggt tcttggccca
    32101 aatttcgatg ttgccgtttt ttataattcg gccggcctgc atcccgagcg gatcctggtt
    32161 gatgtcgagc acttcgttgt tggtgagaag actaaaggtg aaatcatcga gttgggtcat
    32221 atcgcagccg atgagcaggg gggaagccag taatgcccat agagaaatat gtgtgtattg
    32281 ttcattgggc gtcaggtttg tcgggtgtaa tgccgggccc cacccgactt tacctacaac
    32341 cagcatatcc gggtcattcc agtggcccgg gcctgaaaaa tattccagtc cggactggct
    32401 gaaccctatc gtttcaacgc tcgaccagtt atcggtgata tcgccggtag tcctccagca
    32461 gttgccgcct acttcggcgc cccatctcca tacatcgccc attccatact ggcaaaggct
    32521 gtacacaatg tcgcgcccgg ctttatcgag agcctcgcgc atgacgatgt aaggtttctt
    32581 aagctccggc aggctgtggt ctttggcaat gcgctcataa tcgcaccagt catatttaag
    32641 gtaatcgaat ccccattcgg caaattgttt tgcgtcggct tgttcatgcc cgtagcaggc
    32701 tgtatatcct gcacaggttc tggggccggg gccggagtag atccctattt tcagcccttt
    32761 tgaatggaca taatcggtaa gcgctttgac atccgggaat tttttgttgg ttttcatgtt
    32821 tcccttttcg tcgcggggcg ttccccccag gatggaatct ttgctctcgc tttgtatcat
    32881 ccagcaatcg tcaatattaa tataactcca tccgtaatcg gccagcccgc ttttttccat
    32941 ggcgtcggcg gcagccttaa ctttttcctg gtctatggca caggcaaagc agttccacga
    33001 attccaccca agcggcggcg taagcgctat ttgtttgccg gcaacgataa taaatttcct
    33061 tgatgattcc cctttgctgt tttttgcagt aataaccgtt tcataacgac cctcttcagg
    33121 cgtttttccg gtaattaccc ccgttgattt attgaggctt aatcccttgg gcaatccttt
    33181 gacggaaaac ttcatcggac gttccccggt tgcagggata cggtatatga agtcgtttcc
    33241 cggacgggcg ccatagattt tcgggccgtt gatgcgcgga gaagccggag ctttgggcgt
    33301 gaggatatat gcgggctcaa ccggacggct gatagcaaca ggtttttttc ctttaaccag
    33361 gaaataagcg tcagcccagt cggcatgatc gtagttgatg ccgtcgcccg cgccgtcaac
    33421 aatcaacaac aacgtatcga tgccggccgt attcacgttt atttcttctg atttcccgcc
    33481 ggaaaccagt ttgctgcttt ggcagagcaa ttcgccatcg ccgtaaacaa tgaagattat
    33541 gcttccggaa gaagcctttt cttcgtcatc aactccaaca acagcgtgaa agcgttgcgc
    33601 tttaccgcca agtttcaggt acagggtgcc aggcgcatgc gtgccaaacc cggagcggta
    33661 ttgtacacca tttagtatca ggggatttcc catgcaggaa acatttactc ctttctggcc
    33721 ccagccggta gtggccattg atacatcgag cgtcgataac catacggtat cgttaacctg
    33781 tgaatacgct gctgataatg agaataccag tgttacgatt gataaggcaa tgattctgat
    33841 agctttcata cctgttgtgt ttggaaattc aaatttgtta tcctgatggt taagttcatt
    33901 taaaagattc atcaatttct tgttatccgg tgataacttc ccggtaaaag tacaaaaata
    33961 ataatattaa gatatagcct ccaatatatc ggatatgctt attttctgag taccggtatt
    34021 aaagcagttc tggatgttca tcgtgcactt tacagatcag ttcgccaaac agcgtattag
    34081 cccaggcaaa ccaatttctc gtaaaatcag caggattatc cttgttgaag gattcatgca
    34141 taaacccggt gccggcatgc gtattcctga gcatttgaag acaatcggaa atctctgaag
    34201 ggctattgct tgtcatggct tgcatgataa gcgtcattgg ccagatgttc cctccgggcg
    34261 tgtgcggact gccgattccc cgggctgcct ttcctgaaaa ataatagggg ttattctcac
    34321 tcagggcgaa acggcgcgtg ttctgataaa tcctgtcttc cttttcgata caacccaggt
    34381 aaggcattgc aagcaaactg ggtacattgc tgtcgtccat catcaggcgg tttccatagc
    34441 catcggtttc ataacaatag atttcaccat aaaccgggtg ttggattgta gcctggttac
    34501 tgagcgccag ggtcagttct tcgctcaggg ccctgcaccg ctccgcttct ttgaataaat
    34561 tcagatagcc gtatattccg gataattgaa gcaatgactg gcaggcgaaa aagttggaag
    34621 gtatgagata ggggaaaatg gtagcgtcat ccgaaggccg gaaaatggat actataagcc
    34681 cgttgggcct tgcaggattt ccccagccat tgccggggac ggtatcggtt tgccaggatg
    34741 ttgtccgttt gaatgaatat gggccccgcc ccgtttttcg ttgctggtcg cggaatgttt
    34801 gataaaccag ttccatggct ttaagccaat cggagtcaaa aacagaggaa tcgcctgatg
    34861 ccttccaata accgtatgcc agtctgacgg gatagcacaa agaatctatt tcccatttac
    34921 gttcgtgtat gccgggtttc atctccgtaa tatccgattt ccattcactt tcctgattcc
    34981 catcaaggaa agcattggca taaggatcca gcaggataca tttcacctgg cggttaataa
    35041 ctcctgcaat gagttttctt aacggttcat cttttttaat gaacggaata taaggccata
    35101 cctgcgccga tgaatcgcgc agccacatgg catgaatgtc gccggtgatc acaaatgtat
    35161 caggcttccc gtttatttcc gaataagtta ttgtggtatc gagggtgttg ggaaagcagt
    35221 tttcgaacat ccaggctaat tcaggatccc tgataacaga ttttatttcg gcaattcttg
    35281 cttcaaccgc ctggcttaca aacctgcgac tggaaggggc aggccggttg ctgatatatt
    35341 ccttcctgcc gggtaacccg gtggcggttc catagcctcc caacgcaatg cctgctgcgg
    35401 caaggcttgt ggccttaatg aatgtcctgc ggcttgtcat ggtatgttag tatttgagga
    35461 ttatttttac tgatttttcc tttccgttat ctgcttccag gatatatgca ccggacggta
    35521 acatgccggt acaggaggaa atacaatcaa ccatataatg atgtgtgccc gggggaatgc
    35581 agatatcttg caaaacattt ccttgcaggt tctttatccg gaaatttacc ttcaaattca
    35641 aatttcgttt gagatcgatc cggaaactgt ttgaaaacgg attcggacaa accgagaacc
    35701 attcatctga agacgcattg tttccggctg ttcctgtcgt gccattgtat tcatatgcgc
    35761 ccatgtcaat gcggttgttc catatccggg gagcaaatcc cagatcataa gccgggatgt
    35821 tcaggaaagt cgcatccggg gtgcctgcat caatgcaagg agaattcccg gtcaactgat
    35881 aaggaaacaa gcccgcccca taaaaccccg gattttcatc aatgttatac cggtattctc
    35941 cgtgataacc ctggtgtcct ccgcttccct caaagccagc ggtgtcgcct tcaacatcgc
    36001 aatagtaaaa gtccggcgct gacctgatat cccagatata tgcgcttaca cccagacccg
    36061 tattgccgtg aataatggaa ttataaaagg aaggcattgc cgaatcattg caataaattc
    36121 ctccgccgta gcaactcgag ttgttggtta tcgtcaggtt ggagaaaacc ggaaacgacc
    36181 ttatacagga tatccctccg ccaaaaaaca ggcttgtatt gttgacgatt aacaagttag
    36241 agaaagcaga agccggcgaa cagcgcagca ctcccagggc tccgcccaag gcgctgtagt
    36301 tgttttcaaa aatgttattt gtaaaaacag ggtcagcgtc atcaaacgat acgccgccgc
    36361 cgacgccggt ggaagaattc ctcaggaata cattacgggt gatctccggc gaggaatgca
    36421 tggcgcagac tccgccgcca tatccgtaga tatttcccgg gtttcctgca tagttgtctt
    36481 taaaaatgtt gttcctgagg caggcgtctg aattccatag atatacggcg cctcctgaga
    36541 agtaactgtt gttgtgataa aacaggcagt tggttatgcc gattttcccg aaatagcggg
    36601 catagatggc tccgccgtat tcgtcaacag agtcgccgaa agcttttccg aattcaaaac
    36661 ggcagaacga aaacctggag gtgtcattct gagccggcgt ttggttaaac ctgattccga
    36721 accatccgcc gcgctcggtc gacgggttgt ggaaattcgc tgtgtcgcgc accgtaaaca
    36781 ggatcgtatc gccggaattc ccttcagcca ggatccggcc ctgaatgttg atcctgaaat
    36841 gagaccagca tattatccgg gaaccaggcg cgatggttaa tgtctgcccg tcagggacta
    36901 ataagtcgcc catgacaaga accgtatcgg catcccaggt tcctgaaacc ggtccgctaa
    36961 cttcaattga agttgcagcg ctgttaatgg aagcgagagt cagcagaagg aaaggcagga
    37021 tggatagttt tttcatttga agttacggtt tttaaattca taggctcctg caaccgacag
    37081 cccgatggca atcaggaaat aaatgagaga aggcaacgga aacagatcaa gttcatagga
    37141 aggcccccgc ataatgctga acacgtgtga atatgggtag gtatagggat tgtaaagcgg
    37201 aagccatcgt aacacatcag ggttggaaat gagaccggtg acccccagaa tgaacaatac
    37261 ggcaatgggc aatatggcca gcgtcgttcc gatagtgatc ggggccacaa cgttccggag
    37321 cctgaggctt atgaggtact ggatgaccac catcgctgtt ccggcaagga atgttctggt
    37381 gatggctgtg aacaggtaac cgttgtcgaa acccggaaac agcatcaggt tccggtttgt
    37441 taaatgaaga atgatcatcg aagcataggt gagggcaata atcagaaacc acgaaacagc
    37501 catcaggata aaggatgctg ccagcttccc gaaaaaatac gcagatcttg gaaccggcaa
    37561 cgatatcaaa tgtttgaaca tggtattccg gtgctctgtc tggcataaga aactgattgt
    37621 caatacaaca taaaatggaa gaaaaaatgc cgtaacatca ttaatgttgt ttatcgcttt
    37681 tacggggctc atattcgaat ggcccctcat cgaaaacagg aatagcgaag ccagcaggac
    37741 agcgccggca ggatagagta ttgtcagcca gaatgccacc gtgcggcgta attttataag
    37801 ttccgttatg acaaccgttt gaaataagct gattttcttt ttcatgcttc acttccttgt
    37861 ttcaacaatt caaaaaatga ctcttccagc cggtgatctt ctggctttgc agcgtatatg
    37921 ccaattccgg catcaacgag gtttcggatg attgaagcaa tgtcttcctg tgactggtag
    37981 gaaaccgaga cagcgccggg caataagctg gttatgtgag tttcggacat gatttgcaag
    38041 catttcggat tatcactggt tgtcagggtt aagactccgc cggatccgtt ttttcttttt
    38101 aaacccgatg tttctccctg ataaatgatt ttgcccaaat gaataatagc gacatggctg
    38161 caaatttttt caacttcatc gagaatatgg cttgataaaa agatggtaca accttgttcc
    38221 tggttaatcc ggagtaaaag attcctcatt tcacggatgc cctccggatc gaggccattg
    38281 ataggctcgt caagaatcag gagatcgggt ttgtggagca atgccagggc aaggctaagc
    38341 cgttgcttca tgccggttga gaattgcccg acataccggt cggaatctgc tgtgaggcca
    38401 acggtttcaa taacctcatc aaccatcggt gcgggaatgc ccctcaaccg tcgggttatt
    38461 tcaacgtttt tccgggcact caggtgatcg tataacgacg gtacttcaat ggtcgcgcca
    38521 gttcgctgaa gtatatccag ccggtgggaa ttcaactctt tgttaaagat atgaatcatt
    38581 ccgggcttta ctttcaacaa gcccagcaag gcctttatcg ttgttgactt gccggcgccg
    38641 ttgggtccga gaaaaccaaa tatgcttctg cccggcacct ggaggttgag gtggttgagt
    38701 acaggttgcc cgttataggc aaatgtcagg ttttcggtac tgattatgaa tggattttcg
    38761 gtaatgttat ccatgaatta caggttatca gcagattaaa tgaatgtaac ggctatcccg
    38821 gttttgcatc tcccgaataa attccggagg cgaattctga taccaaagta caacgatttt
    38881 atcattagcc cgactgctgc tcattccaaa aggtacagaa aattaatttc ggcattaact
    38941 tgcaaatatc aattatattg tgtaaattta tagagattta aattttgagc catgaaaaca
    39001 aatttaaatt tgataatggt ggcagccttg ctgctgttat cgccgctggc tgttcatccc
    39061 caggtcaagg gaaatccgaa acaagccatc ccggctacaa acgagaacaa gctgaatgcg
    39121 ggtcaaagaa atgttttctc aggttccgga ggactgacga caggtgaggc tgtacttgaa
    39181 ggcactgaag ggaatattta tttcggctcg gaatggccta aaggaatcat caaccttcgc
    39241 gacggttcta cgataacaga acgtggattg cgttatgata tctatgctga ccagatgcag
    39301 atgactgacg ggaaagatac gctggcgctt gcaaaacctg aagaaattaa ttccctctct
    39361 ttcgacgggc atacatttat ctaccagtct tatgaatgtt cgggactggt aaaacaaggc
    39421 tattttgaat tgttggtaac tgggaaatct gaattattgc ttaaacgttc tgtttccttt
    39481 catatcgaag gaaatggcga cgggaaggaa aaatacctga taactgagtg ctactttctg
    39541 aagaaagggg aaagcccggc aataaaaatc atgtgcagca agaagagcgc actggaagtg
    39601 ttttcggaac acagggatga gattgaacgt tatatgaaag agacaggccg gaaagtaaaa
    39661 acgccggaag acctgaaagt cctcgtggag tattacaact cacttgaata ggatttgaaa
    39721 gccggttcaa aatggctttt cttccgttat ctcaggagaa ggtgcggcat aaataaatac
    39781 atttgccgcg ataataacat tacgtaggtg tccgaaatat caggttccct gaaccttctt
    39841 caggaactgc acaattgcag gatttgtccg cgggaatgcg gagccgaccg gttctcggca
    39901 aggttaggct ggtgtaatgc cggggcaggt tttaacatca gttcgatttg tgcgcaccag
    39961 ggcgaagagc cggccatcag tggtaaacaa ggaatatgca atatattttt cagcaggtgc
    40021 aatcttgcct gcatctattg ccagaactgg cagataagca gccggaagga accggctgcc
    40081 gggctaggga tggagttcag cgaagtaatt ggccggattg ttgacctgct cgacggcggt
    40141 tgtcatgccg ttggatttgt ctctccttcg caccatgttc cccaggtaaa agccatcatt
    40201 gaagccgtga gggaatcggg aagaaatccg gtttttgtgt acaacacaaa tggctatgac
    40261 aaggcggaga cgttgaaatc gctggagacg tacatagata tctacctgcc ggatttcaaa
    40321 tacatggatg cctcgctggc aaaagaatat tcggaagccg atgattatcc tgaagtagct
    40381 ctttcggcgc tgaaagaaat gtaccggcag aaaggttcgg tcctgctctg taatcatgac
    40441 ggctatgcag aaaaaggcat tattctcagg catctcattc tgccggggca tacggataac
    40501 agtattgcgg tgctgagaaa aatcgcaggg gatgtctctt catcgttgac gatttcgctt
    40561 atggctcagt attggccggt acctcaggtg gcaagccaca ggcaactggg acgcccgatt
    40621 acacctgagg aatatgaacg cgtgaccagc gaattgcagg cccttggctt ttataaggga
    40681 tggattcagg aattggacag ccaaaacaac tacctgccgg attttaacca gcaacatcct
    40741 tttggaaaag cagtcgggga tggggattcg gaagattgag aatatttggg aaaattgaaa
    40801 ccggatagtc cggtttcagg cttccaggca ccgtgtatca gtctttgggg gtaaaaatgt
    40861 acagcctgcc aggcggtttt tcagtattca attctattct gcgagttggt ccacatgtga
    40921 aaaaccttca cagcttccgg ccgcatcatt tgttctgagg gcaggcgcct gtcttcggcc
    40981 ggaagactga tttcctcata aaaatgctca tcatcgaatc ctgcagcctt ggcgtcatgc
    41041 tggtcggcgg caaaatatac tttatcgatg cgggcccaca gtattgcacc gaggcacatc
    41101 ggacagggct cacaactgca aaaaatttca catcccgaaa ggttaaaagt gcgcagagcg
    41161 acgcatgcat tcctgattgc tacaatttca gcatgggcag taggatcatt tgtttctgtg
    41221 acccggttgc agcctgtcgc gattatttcg ttactgcgaa caactacggc gccgaaaggg
    41281 ccgccattct cattcatgat gttcattccg gcaaggcgga tagcttcagc cataaatttt
    41341 tccttcattg acacctgcat gggataaaat cggatttata aggattatga aactcttctc
    41401 aacaaaaata attcattccg gcattcgtga acatctgtac ctgaaaagtc tgttaatgtc
    41461 gttgccggcg attatgaccc ggaatgcagg gaggagcata cgaaaattgc tgcaggaatc
    41521 gcatggttgc tgtaaaatcg ctaaaaagat actgaccggt ttatgcggtt attaagcccc
    41581 gacgttgtat ttttgcataa aacagacttt gtgttgatga aagcgtgttg caggaatcag
    41641 acggtgaggc ttttgtctgt cttaagaaaa acaacccatg aacttttttt tcactcatta
    41701 caaatcgatt gatggcatgc ggagaatact gatcttaatc ctgttccttt tttcttttat
    41761 tggaatgtat gcacagaatg aaaccgaagc caagcagatg aaggaaaaac ggcaggcggg
    41821 cttctgcatc acatccgatg ccatgatcct gttcaatatg atcaacgatt accgccggac
    41881 gaataatttg ccgttgatac ctctgtcgcg ttcactttcc tttgtcgcca tggtgcatgc
    41941 ctccgatctt caggcaaata atgcagccat ttctgaatgc ggcctgcaca gttggagcga
    42001 taaaggcaac tggaaaccat gctgctatgc cagggatcat gctcaggcat catgtatgag
    42061 taacaagcca aaagaactaa caggataccc cggatcagga tatgaaattg cctattggga
    42121 tgaagaacaa gcaacacctt caggcgcaat ggaactctgg aaatcgacgg ttgtttctaa
    42181 aaatgtcttc cttaaccgtg ataaatggca atcgaaaaaa tggaaagcga tgggggtagc
    42241 tctattggat ggatatgcca ttgtatggtt gggcgataaa gctgattcat tggctgatat
    42301 taccttgtgc ggcactgatt ctcttgtcca gttggcttcg tctccggttc tcgctccggt
    42361 cgccgctgtg aatgaagtcc ctttaaagaa agatatccct gccgtaaaag ctgttgaaaa
    42421 aaaggaaacg ggcccggaga aagaaaagca gaatcagaag caggcaagga aaaatgcaga
    42481 ccagttttat ctggtgattg caagtcttcg tgacgaacaa ttggctgccc gggaggtcga
    42541 aaaacttcag gaaagaggat tttcaaatgc cacagttcag gcaggtgaag atgtgtacag
    42601 ggtgacgata ggggtttatg ctacccgcga agaagcccag aagaaactga ataagtacat
    42661 atcggaattt aaagggatat ggattttaag gcagtaaagt gtatgaaaag cgtgaatctg
    42721 tcagggctta ctgatgatat gtttagcttt tagcctttag cctgccgtct tacgcttagc
    42781 ctgcccatgc aggtaaactg ttagctcaag gtttcagagt ttcaaagttt cagacctgcc
    42841 tgcccggacg aggttgcggg tgaggtggca ggcctgcctg ccttgcgcaa ggctgtggga
    42901 aggtgcaggg tttcagcgtt ttgcgttttg cgttttgcgt tttgcgttct ctgtttggtc
    42961 gttggctcat agctttaagt ttcagggttt caatgtttca aagtttcaga cctgcctgcg
    43021 cgggcgaggt tgcgggtgag gtggcaggcc tgcctgcctt gcgcaaggct gtgggaaggt
    43081 gcagggtttc agcgttttgc gttttgcgtt ttgcgttttg cgttctctgt ttggtcgttg
    43141 gctcatagct ttaagtttca gggtttcaat gtttcaaagt ttcagagttt cagggtttc
//
LOCUS       DAOPYV010000022        42863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_764_length_42863_cov_11.757756, whole genome shotgun sequence.
ACCESSION   DAOPYV010000022 DAOPYV010000000
VERSION     DAOPYV010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 42863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42863
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_764_length_42863_cov_11.757756"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..1222)
                     /locus_tag="PKI35_04425"
     CDS             complement(293..1222)
                     /locus_tag="PKI35_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNX43661.1"
                     /translation="MRRFLLLWLIAAVLPASGQQKKVCISVDDLPVVSYGIQDTVFRK
                     ALINKLIASLKKNDIPAIGFVNEQQLYEDGHPDLFRLSLIKSWVDNGLDLGNHTFSHP
                     DYNKISYEEYTQDIIKGETLTKPILESRGRSLKYFRHPYLHVGDTKAKADSLAGFLSR
                     RGYTVAPVTIDNEDYLFALAYHRAKAKNDTALALRIGHDYLAYMERKALYFERQAGRL
                     FGRDISQILLIHASRLNADYIDSLAMVFRKNGYVFVSMDQALHDEAYQTEVTVYGAWG
                     ISWMDRWALSQGKKRDFFTDDPETPEYIHKLAE"
     gene            complement(1270..2001)
                     /locus_tag="PKI35_04430"
     CDS             complement(1270..2001)
                     /locus_tag="PKI35_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNX43662.1"
                     /translation="MPSAEELIRMNEEERIREAIAPKDWNEIKSHDSWSVFKIMSELV
                     DGYEKLSRIGPCVTIFGSARIAPTDKYYKLAEEIAYILTKKGFGVISGGGPGIMEAAN
                     KGAHFGGGRSVGLNIELPHEQHSNPFIDPDKLLTFDYFFVRKLMFMKYSQGYIVLPGG
                     FGTMDEFFEAVTLIQTHKLVRFPIVMVGVEYWQGLVDWVKSNVLSRKMIEEKDLEIFS
                     LVDDAVSAVNVIEEYYNRYALKPNF"
     gene            complement(2053..4920)
                     /gene="uvrA"
                     /locus_tag="PKI35_04435"
     CDS             complement(2053..4920)
                     /gene="uvrA"
                     /locus_tag="PKI35_04435"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNX43663.1"
                     /translation="METLDPSAISSVSPGRPAGEDNLEIYGARVHNLKNIDVVIPRNK
                     LVVITGLSGSGKSSLAFDTIYAEGQRRYMETFSAYARQFIGNLERPDVDKITGLSPVI
                     SIEQKTTIRNPRSTVGTITEIYDFLRLLYARIGEAFSYSTGERMVRYTEGQIIELISS
                     EFAGKHIHILAPLVKGRKGHYRELLEQVRKQGFLKVRVDGKITDIVFGMQLDRYKVHD
                     IEVVVDTLAVSEKSANRITASVQTAMRHGKGAVIVNDTASGEVRFYSRLLMDPATGIS
                     YDEPEPNTFSFNSPYGYCPRCKGLGEISEIDFNKIIPDKKKSIRKGAIAPVGEYRQSW
                     IFRQLEAIGHKYHFTLDTPVEDIPEQALETILYGSDEVIKVHHESLGVTTSYSLNFEG
                     IVNHILNQNEDSSSEGLKRWAVSFMNTVACPECGGSRLKKESLCFRVDGKDISQLTSM
                     DLVKLSKWFEGLNRRLDEKQSRIANEIIREINNRLGFMLGVGLDYLTLNRKSGSLSGG
                     ESQRIRLATQIGSQLVGVLYILDEPSIGLHQRDNHKLIKALQDLRDSGNSVIVVEHDK
                     DMILSADHVVDIGPGAGNHGGRVVAQGTPVQLMTGKSLTCCYLSGRKEIAVPVKRNKP
                     NGHYLTLTGARGNNLKDVNLRIPLGIFTCITGVSGSGKSSLINETLYPILSRHFYRSE
                     KKPLPYASVEGLENIDKVIEIDQSPIGRTPRSNPATYTGVFDDIRKLFTALPESKIRG
                     YKPGRFSFNVKGGRCETCQGAGVRVIEMNFLPSVHVHCEDCEGKRYNRETLEVRYKGK
                     SINDVLDLTIEQAVEFFANIPSILQKIKTLYEVGLGYITLGQQSTTLSGGEAQRVKLA
                     AELAKRDTGRTIYILDEPTTGLHFEDVNVLLQVLDKLVKRGNTVLVIEHNLEVIKMAD
                     HIIDLGPEGGERGGMIIAEGSPEKIAAMNTGYTARYLKEAL"
     gene            complement(4961..6001)
                     /locus_tag="PKI35_04440"
     CDS             complement(4961..6001)
                     /locus_tag="PKI35_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNX43664.1"
                     /translation="MKMKITPSSIAGFMKKRIVIIVALLTIAGVHGFYLLRNNSGGVD
                     DDNYRKALMHSNKVFSLVLPASVEFAGEEAPLSLYYVREGLDRELTVNTYWHSSTILM
                     LKKAARYFPTIIPILKSYGIPEDFKYLVLIESGLTNVVSPAGAAGFWQFLPETGKRYG
                     LEINDEVDERYHVEKATLAACKMLKDSKATFKNWTLAAAAYNAGESKIMKELERQKVD
                     NYYDLFLNSETSRYIYRLLAIKLLYEHPTQFGFYIRNKDQYPPIPTYRVKVDSTVSNL
                     IDFAKHFNINYRVLKEFNPWLRKDKLTNTARKQYTFLIPKPGYRVYDSLVHSIDNGDM
                     IFNDTITLGTIR"
     gene            complement(6040..6582)
                     /gene="rfbC"
                     /locus_tag="PKI35_04445"
     CDS             complement(6040..6582)
                     /gene="rfbC"
                     /locus_tag="PKI35_04445"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNX43665.1"
                     /translation="MEITKTAIPEVLIIKPDVFGDDRGYFFESYQQEKFKAFGINAAF
                     VQDNESCSQKGVLRGLHFQKPPFAQGKLIRVIRGAVMDVAVDIRKASPTFGKWVSMML
                     TGENKCMCWIPEGFAHGFITLEENTVFSYKCTNYYNKASEGSVRWNDPTLNIDWGTEN
                     PILSGKDSDAPLFSQLPHVF"
     gene            complement(6655..8046)
                     /gene="lpdA"
                     /locus_tag="PKI35_04450"
     CDS             complement(6655..8046)
                     /gene="lpdA"
                     /locus_tag="PKI35_04450"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNX43666.1"
                     /translation="MHYDILVLGSGPGGYVAAIRASQLGFKTGVIEKSEPGGVCLNWG
                     CIPTKALLKSAQVYHYAQQAALYGVKVEGTVRPDFPEIVKRSRGVAEGMSRGIQFLFK
                     KNKIDHIQGFGVVKPGKTITVTSPDGQVADYTADHIIIATGARSRELPNLKQDGRKII
                     GYREAMTLPEQPNRMVVVGSGAIGSEFAHFYNTIGTQVTLVEFLPNIVPLEDEEVSKQ
                     LERSFKKAGMKVMTSSVVESVEIKEDTCFVKIRTPKAEEIVECDIVLSAVGITTNLEG
                     IGLEETGIKTEKGKVVVDDYYRTSVEGYYAIGDIVPGPALAHVASHEGILCVEKIAGM
                     AVEPLDYGNIPGCTYTAPEVASVGMSEKAAREAGYDIRVGKFPFTASGKASAGGSKDG
                     FVKVIFDAGTHQWLGAHMIGDNVTEMIAEAVVARKLKATGHDIINAVHPHPTMSEAVM
                     EAVAAAYGEVIHI"
     gene            complement(8232..8954)
                     /locus_tag="PKI35_04455"
     CDS             complement(8232..8954)
                     /locus_tag="PKI35_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43667.1"
                     /translation="MKRSIHYFAFLMIAIAMVMTSCNKGKKGGASGYEIFGEKSGIVQ
                     YKPFDMMGVKMTQTLYFDEYGAKEMRETVIEGNMMGTAMKQHSIDIRDGNISYHYELE
                     NSAGEGDMAKKEAYKMVLPAEVMEQMNISGFSDKIKTHLDYKEEGKETVAGVKGVKYS
                     IAPDSLNPTNRMIGVHYKNVPLKIVMGQLEMVAEKVEFDVKIPAEKFKVPEGYTIIDE
                     KQMMQQELPGDVPEPQVDSLGK"
     gene            9256..10449
                     /locus_tag="PKI35_04460"
     CDS             9256..10449
                     /locus_tag="PKI35_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX43668.1"
                     /translation="MKHDPAKAIFDLKQFGEFGDVNPSITDSATYTFMQAKTMTDVFK
                     GEQEGCYLYSRHWNPSNKYLADAMAALEGTEQAWVTASGMSAITCAILQLCSSGDHII
                     SSMTTYGGTFAFLQNYATKFNIKTTFVDICDLESVRKAIRPETKLIYTETMTNPMLQI
                     SDIPALAAIANEHGIKLVVDNTFTPMIVQPAVLGAHVVVYSMTKFINGKNDCVAGAIC
                     STADFINSLIDVNNGTSMLLGPVLDPLRSSSILKNLHTLHIRMQQHSRNALYLAGKMK
                     EAGLKVSYPGLPEHKGHELMKKLMNPDFGFGGILTVDLGTSEKAARFMEIMEMHDVGY
                     LAVSLGYFKTLFSNSGRSTSSEVPQEIQDEMGMSEGLVRFSVGLDHDIEATWQRIRHC
                     LDMMD"
     gene            complement(10664..12859)
                     /locus_tag="PKI35_04465"
     CDS             complement(10664..12859)
                     /locus_tag="PKI35_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNX43669.1"
                     /translation="MKAIRNSLFYITTIGVFALVMWLVIRGGQSLESGAKIASGPLNI
                     EQTVQNRDVSGSTGIFESVMEFIQKPLAILILQITIILILSRLFGYLSKKIKQPSVIG
                     EIVAGIVLGPSILGALSPQLFAFIFPVNSIEILHALSQVGLILFMFIVGMELDFNVLK
                     RSAGIAVMVSHASIIFPFALGVGLSYFLYTDFAPANVSFLAFSLFTGTAMSITALPIL
                     ARIIQERGLTNTKLGTMAFTCGAIDDITAWGILAAVIAIVHSGSWLDAGITLILSVIY
                     ISLMVWLVRPFLKKVGRVYITRETLSKQVVAVIFIILLLSAFITEVIGIHALIGAFFA
                     GIIMPKTQEFKKNVIEKIEDVSLVLLMPLFFAFTGLRTEIGLLNEWNLWIVFFQVLIV
                     AVVGKFVGTALAARFGGLSWEISLSLGTLMNTRGLIMLIVLNIGYDFGVISPEMFAIM
                     VLMALITTFMTGPALSFIQYLFKKAGQAEEETAIRPYRIMIAFGPPRMGSVLLNLSRI
                     FLPQNNHEIQPGQISALHLTPSAEVHPTQAAVFEKESFEPVLSTANQCGISLNTIYKV
                     TDDVQSEIIRSARKSQCDLLLLGSAKSIFTSDLLGGKIRRTLHESPCNVGILVDHDFS
                     SATNVIVHYPESPDRMFFALVQNLLQLPSIDTVTIVQQNNNADLEAISALNNSKIRMT
                     DISGFEMTEPAVGTYDLIVVHVNDWKDTIIRKMMKFEKLPSVLILKSRA"
     gene            complement(12968..15844)
                     /gene="gcvP"
                     /locus_tag="PKI35_04470"
     CDS             complement(12968..15844)
                     /gene="gcvP"
                     /locus_tag="PKI35_04470"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNX43670.1"
                     /translation="MITNNFVLRHNGPRPEEAEQMLKTLGVTSLDQLIDETVPGSIRL
                     PKPLNLPKGINEYEYLDHIKKLASKNKVFKSYIGLGYYNTVTPSVILRNIFENPGWYT
                     SYTPYQAEISQGRLEALLIYQTMVSDLTGMPIANASLLDEGTAAAEAMIMFFNARPRE
                     AVKNNANLFLVSDTLFPQTIDVIRTRSKALGIEIKIQPADSFEFTDKTFGAIVQYPDR
                     NGDIRDYSPLAKKAKENHIPLAVAADLLSLTLLTPPGEWGADVVLGSTQRFGIPMGFG
                     GPHAAFFATREEFKRFIPGRIIGISVDAAGNKAFRMALQTREQHIKREKATSNICTAQ
                     ALLAIMAGMYAVYHGPKGIREIARHLNILTGVLAQEAKSYGYTQLNKNFFDTVSFRIP
                     ETTTIKEIRKLAIDSQVNLLYIDDRTIGVSLDETTSLTDVNLVLHILASANRVVFREF
                     VCDPDVCGLIRTFDEKFDRKSEYLHAPEFNSYHSETEMMRYLKKLENRDLSLNRSMIP
                     LGSCTMKLNAATEMIPLSWPEFGSIHPFAPADQTEGYKIMIDELEKALCEITGFKAVT
                     FQPNSGASGEYTGLLVIKAWHESRGEGHRKVCLIPASAHGTNPASAAMAGMEVVVVKT
                     DESGNIDIPDLREKAEKNKDRLAALMVTYPSTHGVFEEGILEIVKIIHENGGQVYMDG
                     ANMNAQVGLTNPGTIGADLCHLNLHKTFAIPHGGGGPGVGPICVAAHLKPFLPGHTFV
                     PGSSQATHAVASAPYGSAFILPISYAYIKMAGGNGLTEATKYAILNANYLKTKLEGSY
                     KILYTGSKGRVAHEMILDCNQFSKTADVQAIDIAKRLMDYGFHAPTVAFPVHGTLMVE
                     PTESEPISELDRLVNAMIAIREEIAEIEEGKADKDINPIKKAPHTVAMVTADEWTLPY
                     TREKAAFPMPCDKQDKYWPAVTRIDDAYGDRNLVCTCSPVSLNQ"
     gene            16075..16986
                     /locus_tag="PKI35_04475"
     CDS             16075..16986
                     /locus_tag="PKI35_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX43671.1"
                     /translation="MKINNAVFAGAMAICFAAVLWGLDGIVLTPRLYRLDITLVVFIF
                     HALPFLIMNLFLFKEYKWLKYFTAGDVLWFSLLALLGGALGTLAIVKALFLVEFKTLS
                     VVVLLQKFQPVFAIALAAFLLREKLRPNFVLWAALAVVAGYFLTFGFHLPDFKTGSNT
                     MLAAGYSLLAAFSFGSSTVLSKKVLQKYSFATATFYRYGFTTLIMLFIAGFSGTLTGI
                     GAVNGKEWLIFLIIAFTTGSGAIFLYYFGLMKIRAMLATICELCFPVSAVLFDYLFNG
                     KVLNPLQWFSAIVMIFAILKLSLQKTE"
     gene            complement(17059..17565)
                     /locus_tag="PKI35_04480"
     CDS             complement(17059..17565)
                     /locus_tag="PKI35_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX43672.1"
                     /translation="MTQIEFSHQLVGLRNNLEYYANMLTSNREDARDLLQDTFLKALS
                     NRDKFASDTNLKAWTYTIMKNTFINNYRRNQKANTIVDSTEDLFYLNIPRKSDFPSPD
                     SLMSEKEINKTIHNLDEEQRLPFEMYNAGYKYKEIADRLNISIGTVKSRIFFTRRKLM
                     DSLKEFSN"
     gene            complement(17855..18739)
                     /locus_tag="PKI35_04485"
     CDS             complement(17855..18739)
                     /locus_tag="PKI35_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNX43673.1"
                     /translation="MKHYSIKDIEKITGIKAHTIRIWEKRYHLVSPERTLTNIRYYND
                     MDMKRLLNIAILNRYGYKISNLVEMSGEEINKRVIEVTLHETDFTSLIESFIVAMIEY
                     DEVRFEKALNSAIIRLGFESAITNVIYPFLAKVGILWQTGVVIPGQEHFVTNLIRQKL
                     ILAIDSRQAIKKNHPRRFILFLPENEFHEIGLLFAHYLLKKYGHNTIYLGQNVPLSDV
                     QQLVSLKKPDFILTSVTTSLSSGDLPEFLKQLSLILPDGQILAGGSQIAYSDNITPAN
                     IVTIKSVKHLTDFLESIH"
     gene            complement(18951..21161)
                     /locus_tag="PKI35_04490"
     CDS             complement(18951..21161)
                     /locus_tag="PKI35_04490"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HNX43674.1"
                     /translation="MNEITRKPIQSLGYNFIPEQYLPEGRDEYYLRNKQNHAANTYRQ
                     LTAHEIEVLVRNRNTTDDWNNILVTEAFNPELVKNSKFFGLVRIGKLEPWFLEFHDLQ
                     VPVGIYNSTIVSCDFGDNVVIDNVNYLSHYIIGDEVIITNVHEIDSTNHAKFGNGILK
                     EGEPESVRIWLEICNENGGRSIIPFDGILPGDAWLWSRSRHNKELQNRFREMTEKQFD
                     NLWGYYSAIGNRAVIKNCSIIKDVKIGDDAYIKGANKLKNLTVNSSPDFPTQIGEGCE
                     LVNGIIGKGCKIFYGVKAVRFVLGSNSQLKYGARLINSFLGENSTISCCEVLNSLIFP
                     AHEQHHNNSFLIAATIEGASNIAAGATLGSNHNSRSPDGEIVAGRGFWPALTASIKHN
                     SYFAPFTLLAKGDYPSELNIPFPFSLVNNNVAENRLEIMPGYWFMYNMYALARNSWKL
                     ADRDKRPEKTQRLEFDYLAPDTVNDMIGGLRLLEKWTGKAAAGIQSPGATFTEEEYIT
                     KGKILLSDSSNFPDDLAVTIEGAEHSKRDCLILKPLQAWQVFSEMIEYYVSENVLGWI
                     EEHEDWSIVKLNEMYEKRLPRSNWLNVGGQLIKSSEVEKLRRQITEYEIISWDQVHRF
                     YNLQGANYPQDRLMHALASFAETSGITKIDSCVLTNLFINYLATRTKLVEGIRKSRLK
                     DYTNPFRKQVYENSEEMEAVLGKIDDTSFIIKQEAELRDAITKINALIENIKTM"
     gene            complement(21315..21887)
                     /locus_tag="PKI35_04495"
     CDS             complement(21315..21887)
                     /locus_tag="PKI35_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43675.1"
                     /translation="MKKLLPLLLFMGLFINAQAQEEMRFQKRDAYFGIGTGLESFSGL
                     IGLTADMRITEKLFIRGGAGIGNWGYKLSAGIRTEKKTTNSVGCGVFVSYATGLNDFT
                     TSLETTSGNKDVKLDLHPVMTLTPEIFYKWLFGRGHRFFISGGYSVPLKTSNWWEVRD
                     GSDLTATSKDVLNIMAPGGLSLGFGFQFVL"
     gene            complement(22123..24399)
                     /locus_tag="PKI35_04500"
     CDS             complement(22123..24399)
                     /locus_tag="PKI35_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43676.1"
                     /translation="MPLFVRFNSAILLLACFSVFSVDAQPAKEHNLRFDNLPARWDEA
                     IPLGNGMIGALVWQKDGKLRISIDRADLWDLRPTKEIEKYTYRWAYEHRLTGDWDTVW
                     KVADEPYDRDPGPTKLPGASVEFDISALGKVKSIELNIASAICSIYWINGASFRIFVD
                     AMNPVVRYSWEGTGPEPVLVPPAYGNSDAKSGKNPVVEGYSLSRLGYEQGTIRKLKNI
                     TVYTQQAWRPLKYQAAVASNQNQGAISITSHYSDRPKAEKASFLVKKAVKSTFDEAAA
                     AHKQWWRDYWSRSSIQIPDKQLEKQWYLEIYKFGASSRKGAPPVSLQAIWTADNGLLP
                     PWKGDFHSDLNTQLSYWPAYSSNHLDEGAVFTDWLWNNKSYFEQYTKRVFDTDGINVP
                     GVATLRGHEMGGWHMYAMSPTVSCWLAQHFYLQWRYSMDRSFLTERAYPWISAAARHI
                     EQITEQKDGIRTLPMSSSPEFHDGGMTAWFLVMTNYDLALTKYIFRIASELATEVGNA
                     QESEHWKEIGKQLPDFDVDPSEGLTVAPGFPMHESHRHFSHLMAFHPLGLLNYEDEND
                     RQIIQNSLDNLEKFGTGAWCGYSFSWLGNIYARMKLGEKAASALREFAACYCSQNSFH
                     LNNSRCKSGFKSEFNGPFTLEGNFAFAAGIQEMLLQSYNGCIEVFPAIPSGWKDVSFQ
                     NLRAEGAFLVSANKENGIPVNFQVESKKGGRTFIKLPFPTYVVKYSQGAKITVNKNHL
                     MEVAFEKGGKIDIENGYE"
     gene            complement(24545..27571)
                     /gene="dnaE"
                     /locus_tag="PKI35_04505"
     CDS             complement(24545..27571)
                     /gene="dnaE"
                     /locus_tag="PKI35_04505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNX43677.1"
                     /translation="MRPKIPPWLYNPSIRSNRFNLFSPFPGPMYLNCHSYYSLRYGTL
                     PIGRLVQTAADHGIEALTLTDINNSMGIVDFVKECGKKGIKPVAGIEFRDENELLYIG
                     IAKNNQGFKELNDFLSRHNIEGIPLPDRPPAFDNCFVIFPFGKFPVSALRDNEFTGIR
                     IPELNLLVSSPYRYRQEKLLALCPLTFTDENGYELHRNLRAIDNNTLLSKLTPVQVAA
                     PDEMLLSPGRIRKHFENYPAILQNAIRLLNECSIGFDFTTVKNKKLFSASAYDDKLLL
                     EKLAMDGLEYRYGKKDKEALKRVKHELEIIDRLQFSAYFLITWDIIRYTMSRGFYHVG
                     RGSGANSIVAYCLRITDVDPIELDLYFERFLNPARSTPPDFDIDYSWKDRDEVFDYIF
                     KRYGREHTALLGATSTFKGKSILRELGKVYGLPKEEIDLLVEEPNNPLNNNDIARHIM
                     SLGVRMTDFPNIRSIHAGGILVSEEPLTCYTALDMPPKAFPTTQWDMYVADDLRYEKL
                     DILSQRGLGHIRECADIIRENRGIAVDVHRIQDFKKDEKVRSQLRVSETIGCFYIESP
                     AMRGLLSKLKCDNYLTLVAASSIVRPGVARSGMMKEYIWRFHNPDKFSYIHPVMEEQL
                     KETYGVMVYQEDVLKICHHFAGMDLADADLLRRAMSGKFRSRVEFQQIVDRFFENCRA
                     RGYPEEITKEVWRQIESFAGYSFSKAHSASFAVESYQSLFLKAHYPIEFMVAVINNFG
                     GFYRTWVYVNEARRCGAKIELPCVNHSHFTTSLTGITVWLGFIHIANLENHLAHAIVE
                     ERNRNGLFLSLEDFTNRVAVGLEQAIILIRLNAFRFTGKTKQKLLWEIHNLLHKNTEL
                     PSTGTLFTTPVRRFELPVLVRSDKEDAFDEIELLGFPVTMSYFQLLQTQYRGEVNARE
                     LSSCTGKQVRMVGMLVTIKYVRTIKNDIMNFGTFFDAEGEFFDTVHFPPTLKNYPFKG
                     RGVYLILGKVVEEFGFASIEVEKMAKLPLVKDERYV"
     gene            complement(27540..28781)
                     /gene="dinB"
                     /locus_tag="PKI35_04510"
     CDS             complement(27540..28781)
                     /gene="dinB"
                     /locus_tag="PKI35_04510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNX43678.1"
                     /translation="MCAEGENIFPADAGSEVPGREAIVHFDLDTFFVSVERLINDKLI
                     GKPVIVGGFSDRSVVAGCSYEARHYGVHSAMPMKMARILCPDAIIVRGDMEQYTRYSN
                     MVTSIIAEQAPVYEKASIDEHYLDITGMDRFFGCMKWTRELRQTIIHETGLPISCGLS
                     VNKTVCKIATGEAKPNGELEIPRDRVIPFLAPLSIRKIPGIGDKTFRLLRSMGIATID
                     TLSRIPPEMIEKVLGRNGLVIWKKANGIDPAPVIPYSERKSISTETTFDQDTIDINMI
                     NDLLVRMVEKTAFTLRKKQKLTSCVTVKIRYSNFDTHTIQQRVPYTSFDHVLIPVTKD
                     LFRRVYQRRMLIRLIGVRFSHLISGTQQIDLFDDTPELISLYQAMDRMRLRYGRGSVI
                     RAVGIGFEEKEDEAEDPAMAL"
     gene            28866..29693
                     /locus_tag="PKI35_04515"
     CDS             28866..29693
                     /locus_tag="PKI35_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HNX43679.1"
                     /translation="MANNFFGQNIKLLRKRRGRTQDDIAVALQMKRPTLSGYENGVAE
                     PGIELLMAFSDYYKISVDTLLKQDLSALPESQLSQLERGFDVFIKGSALRVMATTVDS
                     KNRENIELVPQKASAGYRTGYADPEFIRMLPTFQLPFLSAERKYRMFQISGDSMLPIP
                     DKSWITGEFVQNWQNVRDGLAYIILTIEDGIMFKVVENRIETEGRVRLHSLNPLYEPY
                     YVDVKDIREIWRFVHYISAELPEPNLPKNEFATSLASLKKEMADLKAQLKQGRLFEE"
     gene            complement(29748..32204)
                     /locus_tag="PKI35_04520"
     CDS             complement(29748..32204)
                     /locus_tag="PKI35_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNX43680.1"
                     /translation="MKNALAFIFSFFIVSLAFPQQYPDNSLGEIRKLSKGKNSLVIET
                     TNGVAQVEVYSPEIIRIRINKKLPAGDFSYAVTADLVPCNVKILETEGQILLITDSLR
                     LQISTTPVRFTFTTHDGKTVINEDDGAFGTTWVGEEVTTFKKLQEGEKFIGLGEKTGN
                     LDRRGEGFVNWNSDTPGYTESQDPIYASFPFYIGIHHNVAYGIFFDNTFKSYFNFGAS
                     NDRYSSFGADAGEMNYYFIGGGSVSRIIANYTGLTGRMPMPPIWGLGFQQSRWSYFPD
                     SQVLDIARTFRNKKIPLDVLYLDIHYMDAYKVFTWHPLRFPHPEQMTKDLAGMGIHTA
                     VIIDPGIKVEKGYKAYEDGVKKNMFIKYPDGTDYTAQVWPGWCNFPDFTMPEARKWWG
                     NEFGEIVNDGVTGFWNDMNEIASWGAGKTSSIVRFNWEGKGASYREGKNVYGLLMARS
                     TYEGTKTHMNSRPLVLTRAAFSGAQRYTAIWTGDNVSSDEHMLLGCRLVNSLGISGMP
                     FCGTDVGGFMNGDAGNMLFTRWITIGTFTPFFRVHKHYDFKMSEPWSYGAEAERISRN
                     YISLRYRLLPYLYSAFFEAHTSGIPVNRSLAVTNTFEEKCWYYAYQNQFMFGPSLLAA
                     PVESTKELTKVYLPEGEWYDFFTGKRYEGQQEIIVECPLDKLPVFAKGGSFIPMQSIV
                     QNTAEKPSDTLFLHVYAGNKPGEYTFYEDDGTTFENEKGNFRTQKFSYDPISRKIIME
                     APVGPYAGRFTKIALLLHGFDASEYKINGKKVFAEEITLDLLTSLQENDPLYVWGRKF
                     PQQVKRIMTGNVDEKSVIEW"
     gene            32478..34544
                     /locus_tag="PKI35_04525"
     CDS             32478..34544
                     /locus_tag="PKI35_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNX43681.1"
                     /translation="MKTELPSLRKTMLIDLEKLFPQDAPEPRYVDAFEWSTVKPVVLL
                     MVNTETQYHKTTGEVWVYDTITKKTVMLGKSLRPSGLMHAKLSPDGTKVAYVYQDKSN
                     NKVVYNLYVEDISTGIARQLTFDTRDRVINGTFDWVYAEELSCYDGFRWSPDGTRIAY
                     WNIDASQIRNYLMLNTTDSTYSFTVPVEYPKVGEDPSPAKIGVVDIESAKTIWMDIPG
                     DPRQHYLTRMEWANSQELILQQLTRKQNESKLFIAKSSDGFCRLLSSETDATWIDVES
                     AWNDGDNSGWNWIEKKQAFIWCSEKDGWRHLYRVGMDGRETLLTPGDFDVMKIYCIDE
                     GKKVVYFAASPINHTQQYLYSIPLGGGKAKLLTPEGLPGTHSYMISPNGKWARHSFNS
                     HVFMPVAEWVSLPAHKPLDEGKSISKNLKENPLAKQIEFFQVTTGDGITLDAWMVKPA
                     DFDSAKKYPVIFYVYGEPASSTVLDNAYIGRSSVFGDKVYDRGYLLVSVDNRGTPMPR
                     GREWRKAIYRNIGINNIRDLALGAKEILKRPYCDTSRVAVTGWSGGGSSTLNLMFQYP
                     EIFKTGVSVASVANQLAYDNIYQERYMGVPWETREDFLKGSPYTHAKNLKGNLLYIHG
                     TGDDNVHYANAEKLINELIRYNKPFQMMAYPMRSHGIYEGEGTRDHLKTTCLKFLLEN
                     CPPGGR"
     gene            complement(34679..35818)
                     /locus_tag="PKI35_04530"
     CDS             complement(34679..35818)
                     /locus_tag="PKI35_04530"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX43682.1"
                     /translation="MKHLCITAFLFLSALCQVYSQAILINHTCTDLAAVPAAWLDSAK
                     ANLKVTYQHTSHGSQLVSGINAIASTHGPEFSFTSSGNGLVTDVFLNDYGIPGASDLG
                     HYGDLAWQTATIALLNDPACDRNVVMWSWCGGVSDNDSAGIYAYLNGMNYLEIQYPDV
                     KFVYMTGHLDGSGSEGNLNHMNNLIRNYCAENQKTLFDFADIESYSPGNTQNYMEWCA
                     LDGLNYDPICEDPWGGPNWGVEWVATHSGHQYVTDLLACGECAHSDSPNEAKLNCVLK
                     GNAFWWMMARLAGWNQGASVDENPAAPNLSFIVDHNAKTLTLTGHKASYRKVEIFDMA
                     GRRVSVSEEQNFPQTYLIRLDGLSQGMYVIRAYSAGQILSGKFIL"
     gene            complement(36116..36580)
                     /locus_tag="PKI35_04535"
     CDS             complement(36116..36580)
                     /locus_tag="PKI35_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43683.1"
                     /translation="MKTIRNIIIYTWAVLCMILIPVTFVRSTGFSEMLARLPFMKINP
                     RHSGGEIGRVYEHNNLKITVNKPVFDALIGESRKGFVQVRFSPADSLPPFISDTIDFD
                     NNGTPDLALAIDTKTGQSSLQPIDKNVLDLNISSKVKKDWVVRVNLLNPGRK"
     gene            complement(36592..37755)
                     /locus_tag="PKI35_04540"
     CDS             complement(36592..37755)
                     /locus_tag="PKI35_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX43684.1"
                     /translation="MKTPSAYSTTGKIGKSVLFLLPMLLITFVFITGGQPDFSGTGQV
                     IGFIITYVFFNYLFFRMLYTGKTDRYRAAGFIVLALFFAFVFIVNLVRARGNMSFSDE
                     NILACEIPFCHIVITMIIIPAALTQTIIFPGSILEGFAAISLMIVIWLGISLALGRGF
                     CSWGCFYGGWDDGTSRILKKPVIRKFNPVFRWAAFAVLILVALWAALTLTPQYCNWLC
                     PFKTVTEFEKVTSVETLLKTIVFVSLFAALVIILPLLTKKRIQCATFCPMGALCSLTN
                     KINVFDVKIDKEKCNSCLKCVTICPTLSLNKEEVARGKPSMTCCKCGKCIDSCSRQAI
                     HYHLKGTPSDKKLTTARMLFLYPAFTLLVIFTGGTMQYGITLLIRLFTTGSLF"
     gene            complement(37763..38395)
                     /locus_tag="PKI35_04545"
     CDS             complement(37763..38395)
                     /locus_tag="PKI35_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNX43685.1"
                     /translation="MLSSEGFILGLSTGVVCLAYCGPVLIPYILAESTRVKRNYLSLV
                     LFLGGRLIAYLTTGLIAGLIGMTFFQKSALFTRALAIVYILLALLMIIYGFHRFREIC
                     LGSAGKNVKIHFGPDLTAWVPFLGGVLTGFNLCPPFMMAFTKAFATGNISGSLMLFFW
                     FFLGTSVYFIPLPLIGFVRRQKVLQIIGKFASILAGFYYFYLGLIMLVHK"
     gene            complement(38533..39885)
                     /locus_tag="PKI35_04550"
     CDS             complement(38533..39885)
                     /locus_tag="PKI35_04550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX43686.1"
                     /translation="MIIRDYYMQIFIRLITLVLAGFALCCTWMAHGSVLLLCALSLFT
                     GLVFFNTIKYLHKTDRRLLYFFESVRNDDFTVRFNNDTGDRTLDKLSDKLNELNAGIQ
                     EVRVASQQQDQYLRILLEHAATGIMAFNRKGFIVHCNSSVKKMFGLETFTHIRQLERV
                     NEQLYKTIEALKPDEQKLVSLQQGPVIVQLLLKASYFESTDDAYYIVSAQDIRNELDE
                     KETDAWIKLIRVMMHEIMNTITPITTLSESLSHYLIRDSVNLKPSELGQETVNTLVKG
                     LKVIGDQGQGLIRFVESYRKLTRLPKPEKTLFSIEELFARISELFRSVPGNADIPLMQ
                     KITPPDLEIFADQGMISQVIVNLVKNAFEAGEGYPGLAIRLTAQVSQYNRPEIAVTDN
                     GPGIKPELIEQIFIPFFSTKDDGSGIGLSISRQIMRLHGGSLDVISVPESETVFTMRF
                     "
     gene            complement(39882..41246)
                     /locus_tag="PKI35_04555"
     CDS             complement(39882..41246)
                     /locus_tag="PKI35_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX43687.1"
                     /translation="MPKSRVLIVDDNKSIVTALEILLIPEFDHVRGITNPNLILTELR
                     NCNYNLVILDMNFKAGNNSGNEGLYWLGRILEANPDISVVLITAYKDVEVAVEALKRG
                     ATDFILKPWENSKLLATLKAAVQLNHSRQEVMQLKQKENFLKQEINKTQPAIMGNSPK
                     LMLVLNIVRKVARTNANILITGENGTGKELIAKEIHRLSERSNELLVNVDMGAVSETL
                     FESELFGHVKGSFTDARENRAGKFETANRGTLFLDEIGNLSLHLQAKLLSALQNREVM
                     RVGSDRPIAVDIRLISATNKNLQQMVSDGLFREDLLYRINTIQIEVPPLRERDEDIIL
                     LAEYFIRKYASKYEKANLKINSNAREKLLKYSWPGNVRELDHTIEKAVILSDSNILKP
                     EDFFLQQTSVFRPDEAASSLEEMEKHFIRTVMQKNNGNLSATAGQLGVTRQTLYNKIK
                     KYGL"
     gene            41434..42777
                     /locus_tag="PKI35_04560"
     CDS             41434..42777
                     /locus_tag="PKI35_04560"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX43688.1"
                     /translation="MRFGLIFLILMVASSSLQAQERLNLCRCIRYAIDNNLQIKNIAI
                     SKKLSQERLSQSRRDRLPDAGFYSGAGISFGKSIDPNTNDIISAGFFNSTYNFSSSIT
                     LFDGFRMQKKTDFERFRLNAEEFRLQNAEDELAFDVMTCYYDIQYYKGLTKIARDQAE
                     ASALWLKMIERKAEAGLKSVSDIAEVRANLQLEEVHKVQAENKLNEAMLNLQKLMNVE
                     KPIDTADIEEAPESFPDSTGIMLSGIDPDIIFNSFLLRSPLYLSEEANYKAETKSLSI
                     SRSNFYPAISGGWSIGSGYYETNTTPDGDVISFARQLKDNRNQYLGVSLNFPLFTRMA
                     NRSAVSVARLNAEQARNSMDNTRQNLYFGVCKAVNDLKALQKESLQYVRQQDADSLAL
                     AMAEKKFAQGLISVVDYYVAKNRFANSQSQVLRSALQLDMQARLIAFYEGDRFWE"
BASE COUNT        10575 a         9929 c        10791 g        11568 t
ORIGIN      
        1 aacgagtaga tatacgcaat atttcgctta tatccgtttt atacgaatga tgtctcattc
       61 ccgcacatca atataaatcc ctgctaattt acgcgttttg tttatatatc aaaattatcc
      121 attggacttt tattcgttca aatttccggg cgacggtatg agccaaaaaa atcatggttt
      181 tgctgccgga tggcggggcg acggtgagat aacgcgccag tcagcgcgta ttcaggcaaa
      241 gacggggcaa aaaggcgcca catcttatct ggttaatatg ctaaaggata aattattcgg
      301 ccaacttgtg gatatattcc ggcgtctccg gatcatcggt gaaaaagtcc ctttttttgc
      361 cctgtgacaa cgcccaccgg tccatccacg atattcccca ggcgccgtaa acggtcacct
      421 ccgtttgata agcttcgtcg tgcagcgcct ggtccatact gacgaacaca taaccgttct
      481 tccggaaaac catggccagc gaatcgatat aatcggcgtt gagccggctg gcgtgaatca
      541 gcaagatctg gctgatatcc cttccgaata aacggcctgc ctgcctttca aaatacaggg
      601 ctttccgctc catataagca aggtaatcat ggccgatgcg aagggcaagg gcggtatcgt
      661 ttttggcctt ggcccggtga taggccaggg caaacaggta atcttcgtta tcgatggtaa
      721 ccggtgcaac cgtgtatccg cgccggctca ggaatccggc cagcgaatcg gcttttgctt
      781 tcgtgtcgcc gacgtgcagg tatggatgcc tgaaatattt cagcgacctg ccgcggcttt
      841 ccagtatcgg tttggtaagc gtttcgcctt tgataatatc ctgcgtgtat tcctcgtaag
      901 agatcttatt gtaatcggga tgggaaaagg tgtggttgcc cagatcgagg ccgttatcga
      961 cccagctttt tatcaggctt aacctgaaca ggtcgggatg gccgtcctca taaagttgct
     1021 gttcgttcac gaatccgatg gccgggatgt cattcttttt caacgaggct atcagtttgt
     1081 ttatcaatgc cttccggaaa acggtatcct gaatgccgta actcaccacg ggcaggtcgt
     1141 ccacggagat gcacaccttt ttttgctggc cggatgccgg caacaccgct gcaatcagcc
     1201 ataaaagcag gaaacgtctc atgggaaaat tatttatccg taaaagctga ccgggcagat
     1261 gcctgattat cagaaattgg gtttcagggc gtaccggttg taatattcct cgatgacgtt
     1321 tacggcgctg acggcgtcgt cgacaaggct gaaaatttcc aggtcttttt cttcgatcat
     1381 cttacgggaa agaacattcg atttgaccca atccaccagt ccctgccagt attcaacgcc
     1441 taccatcacg atggggaagc ggactaattt gtgcgtctgg atcagggtca cggcttcaaa
     1501 aaattcgtcc atagtgccga aaccgccggg caatacgatg taaccctgcg agtacttcat
     1561 aaacatgagc ttgcggacga agaaatagtc gaaagtgagg agcttgtcgg gatcgataaa
     1621 gggattggaa tgctgttcat ggggaagttc gatgtttagc cccacggacc gtccgccgcc
     1681 gaaatgtgcg cctttgttgg cggcttccat gatgccgggt ccgccgcccg atatcacgcc
     1741 gaaacctttt ttggtaagga tataggcaat ttcttcggcc agtttataat acttatcggt
     1801 gggagctatg cgtgcagaac cgaaaatggt gacgcacggt cctatgcggg ataatttttc
     1861 atacccgtcg accagctccg acatgatctt gaaaaccgac catgaatcgt ggctcttaat
     1921 ttcgttccag tccttagggg ctattgcttc gcggatacgt tcttcctcgt tcattctaat
     1981 tagttcttct gcacttggca tatttcatca gttttaaagc tttcaaatgt atataaactt
     2041 catcttataa ggttacagcg cttcttttaa ataccgggcg gtataaccgg tattcatggc
     2101 tgcgattttt tcgggcgagc cttcggcgat gatcatcccg ccgcgttcgc cgccttccgg
     2161 gcccagatcg atgatatgat cggccatttt tatcacctcc aggttgtgtt cgatcaccag
     2221 cacggtgttg ccgcgtttta ccagtttatc aagcacctgg agcagcacgt tcacatcttc
     2281 gaagtgaagc cccgtggtgg gctcgtcgag gatgtagatg gtgcggccgg tatcgcgttt
     2341 tgcgagttcg gcggccagtt tcacgcgttg agcttcgcct cccgaaaggg tagtggactg
     2401 ctgccccagc gtaatatatc ccaggccgac ttcgtacagc gttttgattt tctgcaaaat
     2461 ggaagggatg ttggcgaaaa actcaacagc ctgttcgatg gtgaggtcca ggacgtcgtt
     2521 gatggatttt cccttgtacc gtacttcgag cgtttcgcgg ttgtagcgct tgccctcgca
     2581 atcctcgcaa tgtacatgaa cgctgggcag aaagttcatt tcgatgacgc gaacgccggc
     2641 gccctggcat gtttcgcagc gcccgccttt cacgttgaag gagaacctgc cgggcttgta
     2701 accccggatc tttgattcgg gcagggcggt aaaaagtttc cggatgtcgt cgaaaacgcc
     2761 ggtataggtt gctgggtttg agcgcggtgt ccgtccgatg ggcgactgat ctatttcgat
     2821 gaccttgtcg atattttcga gcccttcgac agaggcgtac ggcaacggct ttttttcaga
     2881 acggtagaaa tgacggctca ggatagggta gagggtttcg ttgatcagac tcgacttccc
     2941 gcttcccgaa acgcctgtga tgcaggtgaa gatccccaac ggtatccgga ggttcacatc
     3001 cttaaggttg ttgccgcggg cgccggtaag ggtaaggtaa tggccgttag gcttgttcct
     3061 tttcaccgga acggcaattt cttttcttcc gctcaggtaa caacaggtaa ggcttttacc
     3121 ggtcatgagc tggacgggcg ttccctgggc cacaacccgt ccgccgtggt ttccggcgcc
     3181 ggggcctatg tccaccacat gatcggccga caggatcata tccttgtcat gttcaaccac
     3241 gatgacggag ttgccgctgt cgcgcaaatc ctgtaatgct ttgatgagct tgtggttgtc
     3301 gcgctggtgc agcccgatgc tgggctcgtc gagtatgtac agcacaccca caagctggga
     3361 gccgatctga gtggccagcc tgatgcgttg cgattcgcct cccgacaggc ttcccgattt
     3421 ccggtttaag gtaagatagt cgagaccgac ccccagcatg aaaccgaggc ggttgtttat
     3481 ttcccgtatt atttcattgg caatcctgga ttgtttttcg tccaggcgcc ggttaagccc
     3541 ttcgaaccac ttcgacagct tcaccaggtc catcgaggtg agttgtgata tatccttgcc
     3601 gtcaaccctg aagcataacg actctttttt cagcctgctg ccgccgcatt cggggcatgc
     3661 gaccgtgttc ataaagctca cggcccagcg tttcagacct tcggaactgc tatcttcatt
     3721 ctggttcagg atgtggttga cgatcccttc aaaattcaga gaataggagg tggttacgcc
     3781 gagcgattca tgatgcacct tgatgacctc gtcggaaccg tataaaatag tctctaatgc
     3841 ctgttcggga atatcttcga cgggggtgtc gagggtgaaa tggtatttat gaccgatggc
     3901 ttccagttgc ctgaaaatcc atgattgccg gtactcgcct accggggcaa tagctccttt
     3961 ccggatgctt tttttcttgt cgggaatgat cttgttgaaa tcgatttcgg agatctcgcc
     4021 gaggcctttg caccgcggac aatatccgta tggcgagttg aaggagaaag tattcggttc
     4081 cggttcgtcg tacgaaatgc cggtcgccgg gtccatcagc agacggctgt aaaaccgtac
     4141 ttccccggat gccgtgtcgt ttacaataac ggcgcctttg ccgtgccgca tggccgtctg
     4201 tacagaggct gttatccggt tggcgctttt ttcggaaacg gcgagcgtat ccaccaccac
     4261 ttcgatgtcg tgaactttgt accggtcgag ttgcatcccg aaaacgatat cggtaatttt
     4321 cccgtcaacc ctcactttca gaaagccctg tttcctgacc tgttccagca gttcgcgata
     4381 atggcctttg cgccctttga ccagtggcgc caatatatgg atatgttttc cggcaaattc
     4441 gctgctgata agctctatga tctggccttc ggtatatctg accatccttt ctcccgtgct
     4501 gtagctgaaa gcttcgccga tccttgcata caaaagacgc aggaaatcgt aaatctccgt
     4561 gatggttccg accgtggaac gcggattccg gatggtggtc ttttgttcaa tggaaatgac
     4621 cgggctcagg ccggttattt tatccacgtc gggccgttcc aggttgccga taaactgccg
     4681 ggcgtaagcc gaaaatgtct ccatataccg gcgctggcct tcggcgtaaa tggtatcgaa
     4741 cgcaagcgag gatttgccgc ttccgctcag accggtgatt accaccagct tattgcgcgg
     4801 aataacgaca tcaatatttt tcagattatg gaccctggcg ccatagatct cgagattgtc
     4861 ttcgccggca ggacgtccgg gcgatacaga agaaatggca gaagggtcga gtgtttccat
     4921 gaaggagagg tgacggaagg tttatccccg ccggatttat ttatctgatc gttcccaggg
     4981 tgatggtatc gttaaagatc atatcaccgt tgtcaatgct gtgtacaagg ctgtcgtaaa
     5041 ccctgtatcc cggtttcggg atcaggaaag tatattgttt cctcgccgtg ttggtgagct
     5101 tgtctttccg cagccagggg ttgaattcct tgagaacgcg gtagttaatg ttaaagtgtt
     5161 ttgcaaagtc gataaggttg ctgacggttg aatcaacttt aacccggtaa gtggggatag
     5221 gcggatactg atctttgttc ctgatgtaaa agccaaattg tgtgggatgt tcatacaaca
     5281 gctttatggc cagcaaccgg taaatatacc ttgacgtctc ggaattcagg aacaggtcgt
     5341 agtagttgtc cactttctgg cgttccagtt ctttcatgat cttgctttcg cctgcattat
     5401 aggccgctgc ggcaagtgtc cagtttttaa aggtagcctt ggaatccttc agcattttac
     5461 aggcggcgag ggttgctttt tcaacatgat aacgctcatc aacttcatcg tttatctcga
     5521 gcccgtatcg tttcccggtt tcgggaagga attgccagaa accggcggcg ccggccggcg
     5581 atactacgtt ggtaaggccg ctttcgatca gcaccaggta tttgaaatct tcaggaatgc
     5641 cataactctt cagaatcgga atgatggttg gaaaatagcg ggccgccttt ttcagcatca
     5701 ggatggtgga ggagtgccag taggtattta cggtcaattc gcggtcgagg ccttcccgta
     5761 cgtaataaag gctgagcggg gcttcttcgc cggcaaattc cacactggcc ggaagaacca
     5821 gcgaaaagac cttgttgctg tgcatcaggg ctttgcgata gttgtcgtcg tcaaccccgc
     5881 cagaattgtt gcgcaacagg taaaagccgt gaactcccgc aatcgtgagc aaagcaacga
     5941 tgatgacgat ccgctttttc atgaaaccgg caattgatga aggggtaatt ttcattttca
     6001 tcgggagtaa acaagttttg taccagatta acaagaaggt cagaaaacgt ggggcagttg
     6061 gctgaacagg ggcgcatcgg agtcttttcc ggagagtatc ggattttcag ttccccagtc
     6121 gatattgagc gtgggatcgt tccaccggac agatccttcc gaagccttgt tgtaatagtt
     6181 ggtgcattta tacgaaaata cggtattttc ttcaagtgtg ataaatccat gggcgaatcc
     6241 ttcgggaatc cagcacatgc atttgttttc acccgtcagc atcatggata cccatttgcc
     6301 gaaagtaggc gaggccttgc ggatatcaac ggcgacatcc ataacggcgc ccctgatcac
     6361 ccggatcagt ttcccctggg caaacggcgg tttctggaaa tgcagtccgc gtaaaacgcc
     6421 tttttgcgag caggattcat tatcctgaac aaatgctgca ttgatgccga aggccttaaa
     6481 tttttcctgc tggtacgatt cgaaaaagta cccgcggtca tcgccgaata cgtcgggttt
     6541 gatgatcagc acttcgggga tagcggtttt ggtgatttcc atttgtatca ggataacgtt
     6601 aattcagcgg gttgagtatc atgaagggaa gggttccggc ttatttttca gttctcagat
     6661 gtgtatcact tccccatagg cggcagctac agcttccata acggcttcgc tcatggtagg
     6721 gtgggggtga acggcattga tgatatcatg gccggtggct ttgagcttgc gggcgacaac
     6781 ggcttcggca atcatttcgg tgacattgtc gccgatcatg tgggcgccga gccactgatg
     6841 ggtgccggca tcaaagatca ccttcacaaa tccgtctttt gagccgccgg cgcttgcttt
     6901 gccggaagct gtaaacggaa acttgccgac cctgatatca taaccggctt cccgtgctgc
     6961 cttttcgctc atgcccaccg aagcaacttc gggagccgtg taagtgcaac cgggtatatt
     7021 tccgtaatca agaggctcca ctgccatccc tgctatcttt tcgacacaga gtatgccttc
     7081 gtgcgacgca acatgggcca gcgcggggcc ggggacgata tcgccgatgg cataataacc
     7141 ttcgacgctg gtgcgataat agtcatccac aaccactttg cctttttcgg ttttgatccc
     7201 tgtttcttca aggccaatgc cctcaaggtt tgtggtaatg ccaacggccg aaagaacaat
     7261 gtcgcattca acaatttctt cggctttagg cgtcctgatc tttacgaagc aggtgtcttc
     7321 cttgatttca acactttcca caacggaaga tgtcatcacc ttcatgcctg cttttttaaa
     7381 actgcgttca agctgtttcg atacctcttc atcttcgagg ggaactatgt tgggcaaaaa
     7441 ttccaccagg gtaacctggg tgccgatggt gttatagaag tgggcaaatt cggaacctat
     7501 ggcgccggag ccgacaacca ccatccggtt tggctgttcg ggaagggtca tggcttcgcg
     7561 gtagcctatg attttcctgc cgtcctgttt caggttgggc agctcgcgtg aacgggctcc
     7621 ggtggcgatg ataatatgat cggccgtata atcggcaact tgtccgtcag gacttgttac
     7681 ggttattgtt tttccgggtt ttacaacgcc gaatccctgg atatggtcaa ttttgttttt
     7741 cttgaaaagg aactgtatgc ctctgctcat gccttcggca acgccgcggc tgcgttttac
     7801 aatttcggga aagtcgggcc tgaccgtgcc ttcaactttc acgccgtaaa gtgcagcctg
     7861 ctgtgcgtaa tgatacacct gggcgctctt caggagagcc ttggtaggga tgcagcccca
     7921 gttgaggcaa acgccgccag gttccgattt ctcaatgact ccggttttaa atccgagttg
     7981 tgaagctctg atggcagcta catatccgcc gggaccgctg ccaaggacga gaatgtcata
     8041 atgcataacg aggtatttat tcagtcggtt gcaaaattac caaattccgg ggtaataatc
     8101 ccgtttatac acatacctga acaagtattg tatcgtcaag cgatctgttt ctgacagaaa
     8161 cgggcttgcc gggaaaataa aaaacggctg catcactgag ccgttttctg tatagcgagg
     8221 gggatgaata tctatttgcc aagactgtcg acctggggtt cgggaacatc gccgggaagt
     8281 tcctgttgca tcatttgttt ctcatcgatg atggtatagc cttcggggac tttgaacttt
     8341 tcggcgggga ttttaacgtc gaattccact ttttcggcaa ccatctcaag ctgtcccata
     8401 acaattttca acggaacgtt tttataatga acgccgatca tgcggtttgt gggattcagg
     8461 ctgtcggggg caatggaata tttaacgcct ttaacaccgg caacggtttc tttaccttct
     8521 tccttataat caaggtgtgt ttttatttta tccgaaaagc cgctgatgtt catctgttcc
     8581 attacttcgg caggaagcac cattttatac gcttcttttt ttgccatatc accttctccg
     8641 gcgctgtttt caagttcata gtgatatgaa atgttgccgt cccggatgtc gatgctgtgc
     8701 tgtttcatgg cggtgcccat catattgcct tcgatgacgg tttcacgcat ttctttggcg
     8761 ccgtattcgt cgaagtaaag ggtttgggtc atttttacgc ccatcatgtc aaaaggcttg
     8821 tactggacga tacctgactt ttcaccgaaa atttcgtagc ctgatgcacc gcctttcttg
     8881 cctttgttgc acgatgtcat taccatcgct atggcaatca tgaggaacgc gaaataatga
     8941 attgatcttt tcatggaatc tgttttaaaa ttgactggca aatgtacgat tttagattta
     9001 taaaggtaaa cattcattct ttatggaagg cggcaataaa atcttaatct gtcccggttc
     9061 atctgaatga aaatgaaccg gttccgggga gtttcctgaa aaactgttcc tatcgcccaa
     9121 tcattttaat gccggatgcc ggaatcatcg cacgctgctc ggtaatttta gtaattttgc
     9181 cataccttaa aaaccgggaa atccggcgct gacataaaga attacagttc gttatcaaga
     9241 aatattgaaa atactatgaa acatgatcct gcaaaagcca tattcgatct gaaacagttc
     9301 ggggaatttg gcgatgttaa tccttcgatt accgattcgg ctacatatac cttcatgcaa
     9361 gccaaaacca tgacggatgt ttttaaaggc gaacaggaag gctgctacct ttactcaagg
     9421 cattggaatc cgagcaataa atacctggcc gatgccatgg ctgccctcga agggaccgaa
     9481 caggcctggg ttaccgcatc gggaatgagt gcaataacct gcgccatttt acagctttgc
     9541 agcagcgggg atcatatcat ctcaagtatg acaacctatg gcggaacctt tgctttcctg
     9601 cagaattacg ctaccaagtt taatatcaaa accacctttg tcgatatttg tgatcttgag
     9661 tcggtccgaa aagccatccg tcctgagaca aagcttatct atacggaaac aatgaccaac
     9721 ccgatgcttc agatttccga tatcccggca ttggcagcca ttgcaaacga acatggcatt
     9781 aaactggtag tcgacaacac cttcacccct atgattgttc agcctgcagt gctgggagcg
     9841 catgtggttg tatacagtat gaccaaattc atcaacggca agaacgattg tgttgccggc
     9901 gccatttgca gtacggctga ctttatcaac tcgctcatcg acgtcaacaa cggaacgtcc
     9961 atgctgctgg ggccggttct ggatccgctg aggtcatcca gcattcttaa aaaccttcac
    10021 acgttgcata tccgtatgca acagcacagc cgcaacgctt tataccttgc cggcaaaatg
    10081 aaggaagccg gattgaaagt aagttacccc ggccttccgg aacataaagg gcatgaattg
    10141 atgaaaaagc tgatgaaccc tgatttcgga ttcggcggca tcctgaccgt tgacctggga
    10201 accagtgaaa aggcagcccg tttcatggaa attatggaga tgcacgacgt cgggtatctg
    10261 gctgtcagcc tgggatactt taagaccttg ttcagcaatt ccggccggag tacttccagt
    10321 gaagttcccc aggaaatcca ggatgaaatg ggaatgtcgg aaggcctggt aaggttcagc
    10381 gtaggccttg accatgacat tgaagctaca tggcaacgca tacgccattg cctcgacatg
    10441 atggattaac ataaaaaggc aaagatcaac actgcctgcc ttgcacgcat tcgtggaagg
    10501 caggcgtttt ttttaccggc aaccgccata tattcaggat agcggaggct gatcccgaaa
    10561 tccggaattt tacaggcatc taaacctgcg gtctgtagaa tggtcttgaa aatcagataa
    10621 ttaaactgcc ttcccggcaa atgtgaaacc gccctcttcc ggatcatgct ctgctcttca
    10681 ggatcaaaac tgaaggcagc ttttcgaatt tcatcatctt cctgataatg gtatctttcc
    10741 agtcgtttac atgaacaacg atcaggtcgt aggttcctac ggcgggttcg gtcatttcga
    10801 atccggagat gtcagtcatc cttattttgc tgttgttgag cgctgatatc gcctcaaggt
    10861 cggcgttatt attctgttgt actattgtaa cggtgtcaat agaaggcaac tgtaacaggt
    10921 tttgcaccaa agcgaaaaac atcctgtcag gactttccgg gtaatgaaca atcacatttg
    10981 ttgccgaact aaaatcatgg tctacaagga tgcctacatt acaggggctt tcgtgaaggg
    11041 tcctcctgat cttcccgcca aggaggtcgg aggtaaaaat ggatttagcg cttccaagca
    11101 gtaaaaggtc acactggctt ttcctggcgc tcctgatgat ttcggactgt acgtcgtcag
    11161 taaccttgta gattgtattg agcgaaatac cgcactgatt agcggttgac aggacaggtt
    11221 caaaactttc cttctcaaac acggcggcct gggtggggtg aacttcggcg ctgggagtaa
    11281 ggtgaagggc tgaaatctgt cccggttgaa tttcatgatt attctgcggg agaaatatcc
    11341 tgctcaggtt gagaagtacg cttcccatgc ggggaggccc gaaggcgatc atgatccggt
    11401 aggggcggat ggccgtttct tcctctgcct gtccggcttt tttgaacaga tactggatga
    11461 atgaaagcgc aggacccgtc ataaaggtgg tgatgagggc catcaaaacc atgatagcaa
    11521 acatctccgg tgagatcacg ccgaaatcgt agccgatatt cagtacaatc agcatgatga
    11581 gcccccgggt attcatcagg gttcccagcg aaagggaaat ttcccacgac agcccgccga
    11641 aacgcgctgc cagggcagtc ccgacaaatt tcccgaccac ggcaacgatc agcacctgga
    11701 agaaaacaat ccataaattc cattcgttca gcaggccgat ttccgtgcgc agtccggtaa
    11761 aggcaaagaa caggggcatc aggaggacca ggcttacatc ttcgattttt tcgatgacgt
    11821 tctttttaaa ttcctgggtc ttgggcatga tgatgcctgc aaagaatgct ccgatcagcg
    11881 catggatccc tattacctca gtgatgaagg ccgataacag gagaatgata aatatgacgg
    11941 ccaccacctg tttgcttaat gtttcacggg tgatataaac cctgccgacc tttttaagga
    12001 agggccggac cagccagacc atcaatgaaa tatagatgac agacagaatt aatgttatgc
    12061 ctgcatcaag ccatgatcct gaatggacga tggcaatcac ggcagccagg attccccatg
    12121 ccgtaatgtc gtcgatggcg ccgcatgtga aagccatcgt tccgagtttg gtgttggtga
    12181 gtccgcgttc ctgaatgata cgggccagaa tgggaagcgc ggtaatgctc atcgctgttc
    12241 cggtgaaaag cgaaaaagca agaaacgaaa cattggccgg ggcaaaatca gtatacagga
    12301 agtatgataa accgacaccc agggcgaagg ggaagataat gctcgcatgg ctgaccatga
    12361 cagctatacc ggccgatcgt ttcagtacat tgaaatccag ttccatgcct acaataaaca
    12421 taaacaggat aagcccgacc tggctcaggg catgtaaaat ttcgatggaa ttgaccggaa
    12481 aaatgaaagc gaacaattgc ggggagaggg ccccgagtat ggatggcccc aaaacaatac
    12541 cggcgacaat ttcaccaatc accgatggtt gcttgatctt ttttgacaag tatccgaaaa
    12601 gccgggagag aatcaggatg atggttattt gaaggatgag aatcgccagg ggtttctgga
    12661 tgaactccat caccgactca aagattcctg tggatccgct gacatccctg ttttggactg
    12721 tttgttcaat gtttaatggt ccggatgcga tttttgcgcc tgactccagg gattggcccc
    12781 ctctgattac aagccacatc acaagggcga aaactccaat ggtcgtgatg tagaagagag
    12841 agttccggat tgctttcatc tcaaaggggg agtagggtaa aaaaaagccc tgaatattca
    12901 gggctgcaaa aataacaatt cttataagcg gaaggcggct gatttttttc ggccacctgg
    12961 ccgtttatta ttggttaaga gaaaccggcg agcaggtgca taccaggttg cggtcgccat
    13021 atgcatcgtc aattcgcgta accgcgggcc agtatttgtc ctgtttgtcg catggcatcg
    13081 ggaaggccgc tttttcgcgg gtgtatggca gcgtccattc atcggcagtg accatggcaa
    13141 cggtatgagg cgctttcttg atcgggttga tgtctttatc ggcttttcct tcttctattt
    13201 ctgcgatttc ctcccggata gcgatcatgg cattcaccag gcggtcgagt tccgatattg
    13261 gttcactctc ggtaggttca accatcaggg ttccgtgaac ggggaaggca acggtgggtg
    13321 catggaatcc ataatccatc aggcgctttg ctatatcaat ggcctgaaca tcggctgttt
    13381 tcgaaaactg gttgcagtcg aggatcattt catgcgcaac gcggccttta cttccggtat
    13441 acaggatttt ataagaacct tcgagttttg ttttgaggta gttggcgttc aggatggcat
    13501 atttggtagc ttcggtaagg ccgttaccgc cggccatttt aatatatgca taggaaatgg
    13561 gaagaatgaa ggcgcttccg taaggagccg atgctacggc atgtgttgcc tggctgctgc
    13621 cgggaacaaa tgtatgaccg ggcaggaatg gtttcaggtg ggctgccacg caaatggggc
    13681 cgacaccggg gccgccgccg ccgtgaggta ttgcgaaggt cttatgaagg ttcagatggc
    13741 aaagatcggc cccgatggtt ccaggatttg tcaggccgac ctgcgcattc atgttggcgc
    13801 cgtccatata tacctggccg ccgttttcat gaatgatctt cacgatctca agaattcctt
    13861 cttcaaatac gccgtgggtt gaaggatagg ttaccatcaa cgccgcaagc ctgtccttgt
    13921 ttttttctgc tttttcgcgg agatcgggaa tgtcgatatt cccggattcg tcggttttaa
    13981 ccacaactac ttccatgcct gccatggcgg cactggccgg atttgttccg tgtgctgatg
    14041 caggaatcag gcaaaccttg cgatggcctt ctccgcggct ttcatgccat gccttgatga
    14101 cgagcaaacc ggtatattcg cctgaagcgc ctgaattggg ctggaatgtg acggctttaa
    14161 aaccggtgat ctcgcataac gccttttcca gttcatcaat catgatcttg taaccttcgg
    14221 tctggtcggc aggggcaaac gggtgaatgg aaccgaattc gggccagctc agggggatca
    14281 tctcggtggc ggcgttaagc ttcatggtgc aggaaccgag ggggatcatg ctgcggttga
    14341 gcgacaggtc gcggttttcg agtttcttga gataccgcat catttcggtt tcggaatgat
    14401 agctgttaaa ctcaggcgca tgcaggtatt ccgatttcct gtcgaatttt tcgtcgaagg
    14461 tgcgaatgag gccgcaaacg tccggatcgc agacaaattc cctgaatacc acccggttgg
    14521 ccgaagccag aatgtgcagc acaaggttta catcggtaag cgaagtggtt tcgtcgaggc
    14581 tgacgccgat ggtgcggtca tcgatgtaca acaggttaac ctggctgtcg atggcaagtt
    14641 tgcggatttc ttttatggtt gtggtttccg gaatgcggaa gctgaccgtg tcgaagaaat
    14701 ttttgttcag ttgggtataa ccgtatgatt ttgcttcctg ggccagaact ccggtaagga
    14761 tattgaggtg gcgcgcaatt tcccggatgc cttttggacc gtgatatacg gcatacattc
    14821 ctgccatgat ggccagcaat gcctgggctg tgcagatgtt ggaagtggct ttttcgcgtt
    14881 tgatatgttg ttcgcgcgtt tgcaacgcca tgcggaaggc tttgttgccg gcggcatcaa
    14941 ccgatatccc gatgatacgg ccggggataa aacgcttgaa ttcttcacgt gtggcgaaaa
    15001 atgcggcgtg cggccctccg aaccccattg ggatgccgaa acgctgtgtt gatccgagaa
    15061 ccacatctgc gccccattct ccgggagggg tcagaagggt aaggctcaat aaatctgcgg
    15121 caacggctaa cggaatatgg ttttcctttg ctttttttgc tagcggggaa taatcacgga
    15181 tgtcgccatt ccggtccggg tattgaacga tggcgccgaa agtcttgtca gtaaattcaa
    15241 aggaatcggc aggctgtatt tttatttcaa tacccagcgc tttggacctt gtacggatca
    15301 catcaatggt ctgagggaag agcgtatcgg aaacgaggaa aagattggca ttgtttttaa
    15361 cggcttcgcg ggggcgggcg ttgaaaaaca taatcatggc ttcagcggcg gcagtgcctt
    15421 catcaagcag cgatgcgttg gcaatgggca tcccggtgag atcgctgacc atagtctggt
    15481 aaataaggag cgcttcaagg cggccctgcg aaatttcagc ctggtaaggg gtataggatg
    15541 tataccatcc ggggttttca aaaatattcc gcagtatcac tgagggcgtg acggtgttat
    15601 aataaccgag cccgatatag gatttgaaaa ccttgttctt cgaagccagt ttcttgatat
    15661 gatcaaggta ttcatactca ttgatgcctt ttggcaggtt aagcggtttt ggaagtcgga
    15721 tggaaccggg aacggtttca tcaatgagct ggtcaagaga agtaactcca agcgttttaa
    15781 gcatttgttc ggcttcttca ggacgaggac cattatgacg aagaacgaaa ttattggtaa
    15841 tcatgaggtt atgtatttat tttggatttt actagttttc ggcaaaggta gtaatcttgc
    15901 cattatataa gttatgatgg ccgaacgatt gttagtgaaa taacagaatt gttcataaat
    15961 tgtccctgtc ttactattga taaggtatca acggtttttg acaattttcg atacttttgc
    16021 agtcgccgga tttattaaat caggagactg gttcataata atttattcat cgtgatgaaa
    16081 ataaacaatg ctgtttttgc cggcgccatg gctatttgtt tcgctgccgt attatggggg
    16141 ctcgatggta ttgttctgac gccaaggctt tacaggcttg atattacgct ggtcgttttc
    16201 atttttcatg ccttgccttt cctgatcatg aatttatttc tctttaagga atataaatgg
    16261 ctgaagtact ttacagccgg tgatgttctc tggttttctt tattggcctt acttggaggg
    16321 gcgctgggga cgctggctat tgtaaaggcc ttatttctgg ttgagtttaa aacattatcc
    16381 gtggtagtcc tgctacagaa gttccagccg gtatttgcga ttgcgctggc agcatttctg
    16441 ctcagggaaa agctccggcc gaactttgta ctttgggctg cactggcagt tgtggcgggt
    16501 tattttctta cgttcgggtt ccatttgcct gactttaaaa ccggaagcaa tacgatgctt
    16561 gccgccggtt attcattatt agccgccttt tcattcggga gttctacggt gctaagcaaa
    16621 aaagtccttc aaaagtattc atttgctact gccacctttt accgctatgg gtttaccacc
    16681 ctgattatgt tgtttatcgc cgggttttcc ggaaccttaa ccggaattgg cgccgtgaac
    16741 gggaaagaat ggctgatatt cctgatcatt gcatttacta caggatcagg ggctatattt
    16801 ctctattatt tcggactgat gaaaataagg gctatgctgg cgacaatctg tgaattatgt
    16861 ttccctgtgt cggccgtgct cttcgattat ctgttcaatg gcaaggtgct aaatccctta
    16921 caatggttca gcgctatcgt tatgatcttt gccatcctga aacttagcct tcagaaaacg
    16981 gaatgaacaa actatgctac cccggtgctg caggtgccaa aaggcaaaaa cagtattaga
    17041 gtataacaat acttaaaatt aatttgagaa ttcttttaat gaatccatca attttctgcg
    17101 ggtgaagaaa atccggctct ttactgttcc gatggaaatg tttagccggt cggcaatctc
    17161 tttgtattta tagccggcgt tgtacatttc gaaaggaagg cgctgttctt cgtcaaggtt
    17221 atggatggtt ttgttaattt ccttttccga catgagtgaa tccggggacg gaaagtcgct
    17281 cttccgggga atattcaggt aaaacaaatc ttccgtagaa tcaacaatgg tattagcttt
    17341 ttgattcctg cgataattat tgataaaggt atttttcatg atcgtatagg tccaggcctt
    17401 gagattggta tccgatgcga atttatcccg gttcgagagc gccttgagaa atgtatcctg
    17461 aagaagatcc ctggcatctt cccgattcga agtaagcatg ttggcataat actccaggtt
    17521 gttccgtaaa ccgactagct ggtgggagaa ttctatctgt gtcattgttt ttagtgtttg
    17581 gttaatgctt aaaaacgtac ttgaatactt aattaaattc aattttaaaa catttcccgg
    17641 gtagtgcatt ttcctgaagt gattgatatt caggttttaa aaaggatagt gatccttatt
    17701 tggaaacgtt ttaaattgtt gactaaatta caatgaaaac taaacaaaac aataggttca
    17761 gttaaaaaaa atgttaaaaa attcttaaga ggataaacac ggtcagaatc aatcagatag
    17821 aagaccgacg gatattggga acacagaaaa tgattcaatg gattgattcc agaaagtcag
    17881 taagatgttt gacggatttg atcgtcacga tattggcggg ggtgatgtta tcagaataag
    17941 ctatttgcga accgccggca aggatttgtc cgtcaggcag tatcagggag agctgtttca
    18001 gaaactcagg caggtcgccg gaggagaggg atgtagtaac ggatgtcagg ataaagtcgg
    18061 gctttttaag ggagacaagt tgctgaacgt cggaaagggg aacattttgc ccgagataaa
    18121 tcgtattatg gccgtatttc ttcaacaggt aatgggcaaa tagcagtccg atctcatgaa
    18181 attcgttttc gggcaggaag agaataaacc ggcggggatg gttttttttg atggcctggc
    18241 gcgaatcgat ggccaatata agtttctggc ggataaggtt ggtcacaaaa tgttcctgcc
    18301 cgggaattac gactccggtt tgccatagta tacctacttt ggcaagaaac ggatagatga
    18361 catttgtaat tgcactctca aatcccagcc tgataatcgc gctattgagc gctttttcaa
    18421 acctcacttc atcatattcg atcatggcaa cgatgaagct ttcaataagg gaagtaaaat
    18481 cagtttcatg cagtgtgacc tcgattacgc gcttgttaat ttcttcaccg ctcatctcca
    18541 cgagatttga gatcttatag ccatacctgt ttaaaatggc aatattgagc aaacgcttca
    18601 tatccatatc gttgtaataa cggatattgg taagcgtgcg ctcaggactt accaggtgat
    18661 aacgcttctc ccaaatgcgt atggtatggg ccttgatccc tgtgattttc tctatgtcct
    18721 ttatggagta atgtttcatc cgtaatgagt ttaacagatc ggatggtact ccatgtctgc
    18781 agctttcgga agctccgatg cggctgttgc aaacatgtcc gtttcatatt gtgttcaaca
    18841 tccagtcaac cagaagttaa aacaatattt gcagcaaaaa gtttcagggt acgagtgccc
    18901 gtcaaagggt tatagtaatc cgagccggaa aagggcagtt ttccgggatt ctacatggtc
    18961 ttgatattct caatcagggc attgattttg gtaatggcat cccggagttc agcttcctgt
    19021 ttaatgatga acgatgtatc gtcaattttc cccagtacgg cctccatttc ttcggaattt
    19081 tcataaacct gtttcctgaa aggattcgta taatccttga gccgggattt acggatgcct
    19141 tcaaccagtt ttgtacgcgt ggcgaggtag tttataaata agttcgtcag gacacaactg
    19201 tcgattttgg tgatgccgga tgtttctgca aacgaagcca gggcgtgcat cagcctatcc
    19261 tggggataat tcgcaccctg aagattgtaa aaccggtgga cctggtccca gcttataatc
    19321 tcgtattcgg tgatctgccg tctcagcttt tcaacttcgg atgattttat cagttgaccg
    19381 cccacattca gccagttgct ccggggaagc cgtttttcat acatctcatt gagtttcacg
    19441 atactccagt cttcatgctc ttcaatccag ccgagcacat tttcggaaac atagtactca
    19501 atcatttcac tgaaaacctg ccaggcctgt aaaggtttca gtataaggca atcccgtttg
    19561 ctgtgctcgg cgccttcgat ggtcacggcc agatcatcgg gaaagtttga agaatccgaa
    19621 agtaagattt tccctttcgt aatgtattct tcctctgtaa acgttgcccc gggcgactgt
    19681 atgccggctg ccgctttgcc tgtccatttt tccagaagcc gtaagcctcc gatcatgtca
    19741 ttgaccgtgt cgggtgcaag gtagtcgaat tcaaggcgct gcgttttttc aggccttttg
    19801 tcgcggtcgg caagtttcca cgaattgcgg gccagggcgt acatgttgta catgaaccag
    19861 taaccgggca ttatctccag ccggttttcg gcaacattat tgttaaccag cgagaaaggg
    19921 aagggaatat tcagttcgga agggtagtct cctttggcca gcaacgtaaa aggtgcaaaa
    19981 tagctgttat gtttgatgct ggctgtcagg gcaggccaga agccacgtcc ggcaactatt
    20041 tcaccgtcag ggctccggct gttgtgattg gagccgaggg tggcccctgc ggctatattg
    20101 ctggcccctt cgatggttgc agcgatcagg aacgagttgt tatgatgttg ttcgtgggcc
    20161 gggaagatca gtgaattgag aacctcgcaa catgatatgg ttgagttttc accgagaaag
    20221 ctgttgatga gcctggctcc gtattttaac tgcgaatttg atcccagcac gaacctcacg
    20281 gcttttaccc cgtaaaatat cttacagcct ttgccgataa tcccgttgac cagttcacag
    20341 ccttcgccga tctgcgtcgg gaaatcaggg gatgaattaa ccgtgaggtt tttcagcttg
    20401 ttagcccctt tgatataagc atcgtcgccg atctttacgt ctttaatgat gctacagttc
    20461 ttgataaccg ccctgttgcc gatggcgctg taatatcccc aaaggttgtc gaactgtttt
    20521 tcggtcattt cgcggaaacg gttctgcaat tccttattgt gccggctccg gctccaaagc
    20581 caggcgtcgc cgggcaagat gccgtcgaag ggaatgatgc ttcttccccc gttttcgttg
    20641 catatctcga gccagatacg gaccgattca ggttcgcctt ctttaagaat gccgttgccg
    20701 aatttggcat ggttggtcga atcgatttca tgcacattgg taatgattac ttcgtcgccg
    20761 atgatataat gcgagaggta attcacgtta tcgatcacca cattgtcgcc gaaatcgcag
    20821 cttacaatgg tgctgttgta tattccgacc ggaacctgca ggtcatgaaa ctccagaaac
    20881 cagggctcaa gtttgccgat gcggaccaat ccgaaaaact ttgaattttt taccaattcg
    20941 ggattaaagg cctcggtgac taaaatgttg ttccagtcat cggtagtatt ccggttgcgg
    21001 acaagcactt caatttcatg ggcggtaagt tgccggtatg tattggcagc atgattctgt
    21061 ttgttgcgaa ggtaatattc atctcttcct tccgggagat attgttcagg aataaaatta
    21121 taccctaacg actgaatagg ctttctggtg atctcattca taagttgaag aaatagcagg
    21181 ataaaggtac aacaatattt gtcctgatgt tttaggactt cgctataatt cctgctttcc
    21241 ttgcatcgtc cgggaagcaa tcagcaatta ttcaggaaga taatcctgaa ataaagaaag
    21301 aaattattat cctgtcagag tacaaactgg aaaccaaacc ccagggatag tccgccggga
    21361 gccataatgt tcagcacgtc tttgctggtt gcagtcaggt cagaaccgtc cctgacttcc
    21421 caccagttgc tggttttaag ggggacagaa tatccgccgg aaatgaaaaa ccggtgaccc
    21481 cggccgaaca gccatttgta aaaaatttcc ggggtgaggg tcatcaccgg atgaaggtcc
    21541 agtttaacgt ccttgtttcc cgaagtagtt tccagggatg tggtgaaatc gtttagcccc
    21601 gtcgcatagg aaacgaaaac accgcaaccg acgctgttcg tggttttttt ctccgtccgg
    21661 atgcctgcac tgagtttata accccagttg ccgattccgg cgcctccccg gataaacagt
    21721 ttttcggtaa tcctcatatc ggcggtgagg ccaatcaatc ctgaaaaact ctcaagaccg
    21781 gtaccaatgc cgaaataggc atctctcttc tgaaatctca tttcttcctg ggcttgtgcg
    21841 ttgataaaca gacccataaa aagcaataat ggaagtaact ttttcataat aacggatgaa
    21901 tgaagtgagt aaaaatgtga aatgattcct gttgaatagt ttttatcaaa aataaaaaaa
    21961 attgaatgat ggagtagaat caagtctgaa tttgttgatg ataaaaatct gtacaaaatc
    22021 acgccgtgac atttgggctt ggtgaagaaa tttttaacac atccccagga atgaaaaaag
    22081 gctgatttga gttttgttcg atcgtgacaa acgtcagagt cattactcat atccattctc
    22141 aatatcaatc ttaccgcctt tttcaaacgc tacttccatc agatggtttt tgttgacggt
    22201 tatttttgct ccctgtgaat attttacaac ataagtaggg aacggcaatt tgataaaggt
    22261 tcttccgccc tttttacttt caacctgaaa gttaacagga atgccgttct ccttgtttgc
    22321 cgaaaccagg aatgcgcctt ctgcccttaa attttgaaag gatacatctt tccatcccga
    22381 aggtatggcc ggaaagacct cgatgcagcc gttatagctt tgcaggagca tttcctgaat
    22441 tccggcagca aaggcgaagt tgccttcgag ggtaaaaggt ccgttgaatt cacttttaaa
    22501 tcctgatttg catcggctgt tattcaggtg aaagctgttt tgggagcagt aacaggctgc
    22561 gaattctctc aaggccgatg cggctttttc gcctagtttc atgcgggcat aaatatttcc
    22621 aagccagcta aaggaatatc cgcaccatgc gccggttccg aatttttcga gattgtccag
    22681 gctgttttgt attatctgcc tgtcgttttc gtcctcataa tttaacaatc ccagcggatg
    22741 gaatgccatg agatgtgaaa aatggcggtg cgactcgtgc atcggaaagc ccggggccac
    22801 agtgaggcct tcggaaggat cgacatcaaa atcaggcagt tgtttcccga tttctttcca
    22861 gtgttccgat tcttgggcat tccctacttc tgtggccaac tcagaagcaa ttctgaatat
    22921 atatttcgtc aatgccaggt cgtaattggt cattaccagg aaccatgccg tcatgccgcc
    22981 gtcatggaat tcgggactgg aactcatggg aagcgtccgg atgccgtctt tttgttctgt
    23041 gatctgctcg atgtgacggg ctgctgccga tatccaggga taagcccttt cggtaaggaa
    23101 ggagcggtcc atgctgtacc gccattgcag atagaaatgt tgggcgagcc agcaactcac
    23161 ggtcggcgac atggcataca tgtgccagcc tcccatttca tgtccccgca aggtggccac
    23221 tcccggaaca ttgataccgt cggtgtcgaa aacacgtttt gtgtattgtt caaaatagct
    23281 tttattattc cagagccagt cggtgaaaac ggcgccttcg tcaaggtggt tgctgctgta
    23341 tgccggccag tagctgagct gggtgttgag gtcgctgtga aaatcccctt tccagggcgg
    23401 caacaggccg ttatcggctg tccagattgc ctgcagcgaa acaggcggcg ccccttttcg
    23461 ggaggatgcg ccgaatttgt aaatttcgag gtaccattgt ttttccaact gtttgtcggg
    23521 aatctgaatt gaggaccggg accagtaatc ccgccaccat tgcttgtggg cagccgctgc
    23581 ttcgtcaaac gtagatttga cagccttctt cacaaggaaa gaagctttct cagctttcgg
    23641 gcgatcgctg taatgcgaag taatgctgat ggcgccctga ttttggttcg atgcaaccgc
    23701 cgcctggtat ttgagcggtc tccaggcttg ttgcgtataa acggtgatgt tttttaattt
    23761 cctgatcgta ccctgttcat acccgagccg ggaaaggctg tatccttcaa caacagggtt
    23821 tttcccggat tttgcatcgg aattgccata agcaggcggg acaagaaccg gttcagggcc
    23881 tgtcccttcc catgaatagc ggacgaccgg attcatggca tcgacgaaaa tccggaagga
    23941 ggcgccattt atccaataaa tgctgcaaat agccgaggca atgttcagtt cgatggattt
    24001 tacctttccc agggcgctga tgtcaaattc taccgaggcg ccgggaagct ttgtaggccc
    24061 gggatcgcgg tcgtaaggct cgtcggcaac cttccatacc gtgtcccagt caccggtaag
    24121 cctgtgttca taagcccagc ggtaggtgta tttttcaatt tcctttgtgg gccttaaatc
    24181 ccaaaggtcg gcgcggtcaa tggaaatacg caactttccg tccttttgcc acaccagcgc
    24241 cccgatcatg ccgttgccca ggggaatggc ttcatcccat cgggccggca ggttatcgaa
    24301 ccgaaggtta tgttctttgg ccggctgggc gtcaacggaa aagacgctga aacaggcaag
    24361 aagcaggatt gctgaattaa aacggacgaa gaggggcatg gcttccggtt gaaaaatgaa
    24421 tatcagacaa atttataatg tttccggaga gaacatgatg atttatctga tatatccggc
    24481 gagtttacga cctgccataa cctgtattcg tccgggaaac caaattctga ttgaagaaga
    24541 cggattatac atatctctca tctttcacca gcggcagttt ggccattttc tccacttcga
    24601 tactcgcgaa gccgaattct tccaccactt ttcccaggat caggtagacg ccccggcctt
    24661 tgaagggata gtttttcagg gtgggaggga agtggaccgt atcgaaaaat tcaccttccg
    24721 cgtcgaagaa agtgccgaag ttcatgatat catttttgat ggtgcgtaca tatttgatgg
    24781 tcaccagcat gcccaccata cgtacctgtt tgccggtgca gcttgaaagt tcccgggcat
    24841 ttacttcgcc ccggtattgg gtttgcagca gttgaaaata actcatggtg accgggaatc
    24901 cgagcagctc aatttcatcg aaggcgtctt ccttatcgga acgtaccagt acagggagtt
    24961 caaaccttcg taccggcgtg gtgaacaacg ttcccgttga aggcagctcc gtatttttat
    25021 gtaacaggtt atggatttcc cagagtaatt tctgctttgt ttttccggta aacctgaaag
    25081 cgttaaggcg gataaggatt atggcctgtt ccagccctac agctacccgg tttgtaaagt
    25141 cttccaggct taggaacaat ccgttccggt tgcgttcttc aacaatggca tgagcaagat
    25201 ggttttcgag gttggcgata tggataaatc caagccagac ggtgatgccg gtgagcgagg
    25261 tcgtgaaatg gctatggttt acgcaaggca gctcaatctt tgccccgcac cgtcttgcct
    25321 cattcacata tacccaggta cggtaaaaac ctccgaaatt attgatgacg gcaaccatga
    25381 actcgatggg gtagtgggct ttcaaaaaaa ggctctggta actttccacg gcaaaggaag
    25441 ccgaatgggc cttggagaaa gaataaccgg cgaacgactc gatctgccgc cacacttctt
    25501 tggtgatttc ttcgggatat ccgcgggccc ggcagttttc aaagaatctg tcgacgattt
    25561 gctgaaattc caccctcgat ctgaattttc cgctcatggc acgtctgagc aggtcggcgt
    25621 cggcaagatc cattcccgca aaatgatggc atattttcag cacatcttcc tgataaacca
    25681 ttaccccgta agtttctttg agttgttctt ccatcaccgg gtgaatgtag ctgaacttat
    25741 ccggattgtg aaagcgccat atgtactctt tcatcattcc cgaccgggct acgccgggac
    25801 ggacaatgct gctggcagcc accagggtga ggtaattatc gcatttcagc tttgatagca
    25861 gcccccgcat ggccgggctt tcgatgtaga aacacccgat ggtttcactc acccgcaact
    25921 gggaacgtac tttttcatcc tttttgaaat cctgtatccg gtgaacatcc accgcgattc
    25981 cccggttttc ccgtatgatg tccgcgcatt ccctgatatg acccaatccc cgctggctca
    26041 ggatgtcgag tttttcataa cgcaggtcat cggcaacgta catgtcccac tgggtcgtgg
    26101 ggaatgcctt cgggggcata tccagcgccg tatagcaggt gagcggttct tccgaaacca
    26161 ggatcccgcc ggcatggatg ctgcggatgt tcgggaagtc ggtcatcctg acgccaaggc
    26221 tcataatgtg gcgggcaatg tcgttgttgt tcagcggatt gttgggctct tccaccagca
    26281 ggtcgatttc ttctttgggc aatccgtaaa ccttgccgag ttcacgcagg atggatttcc
    26341 ctttgaaagt ggaggtcgcc cccagcaggg cggtgtgttc ccgtccgtag cgtttaaaga
    26401 tgtagtcgaa cacttcgtcg cggtctttcc aggaataatc gatgtcgaaa tcgggtggag
    26461 tgctgcgtgc cgggttcagg aaacgttcga aatagaggtc cagctcgatg gggtccacat
    26521 cggttatgcg caggcagtaa gccacgatgc tgttggcgcc gctgccccgg cctacatggt
    26581 aaaacccgcg gctcatggta tagcggatga tgtcccaggt gatcaggaaa taggcggaga
    26641 actgcaggcg gtcgatgatt tccagctcgt gtttcacccg tttcagggct tccttgtcct
    26701 ttttgccgta gcggtactcc aacccgtcca tggcgagttt ttcaagcaga agcttgtcat
    26761 cgtaagcgct ggccgagaac agttttttgt ttttaacggt ggtaaaatcg aaaccgatgg
    26821 aacattcgtt cagcaggcgg atggcgtttt gcaggatcgc cgggtaattt tcgaagtgtt
    26881 tcctgatcct gccgggagag agcaacattt catcaggcgc tgcaacctga accggcgtaa
    26941 gtttgctgag aagcgtattg ttgtcgatgg cccggaggtt ccggtgaagt tcgtagccgt
    27001 tttcatcggt aaacgtcagc gggcaaaggg ccaggagttt ttcctgacgg taccggtatg
    27061 gcgatgaaac caataagtta agttccggaa tccggatgcc ggtaaattca ttatcgcgca
    27121 acgccgatac gggaaatttt ccgaaaggga atattacgaa acagttgtca aatgccggcg
    27181 gacggtcggg gaggggaatg ccttcgatgt tatgccggct taggaagtcg ttgagttctt
    27241 taaatccctg gttatttttt gcaatgccga tatagagcaa ttcgttttca tcacggaatt
    27301 cgatgcctgc caccggttta atgccttttt tgccgcattc ctttacgaaa tcaacgatgc
    27361 ccatgctgtt gttgatatcg gtaagcgtta aagcctcaat gccatgatct gcggcggttt
    27421 gcaccagacg gccgatgggc agcgttccat aacgcagact gtaatatgaa tgacagttga
    27481 ggtacatcgg gccagggaaa gggctaaaaa gattgaagcg attggagcgt atagaagggt
    27541 tataaagcca tggcgggatc ttcggcctca tcctcttttt cttcaaatcc tattcccacc
    27601 gccctgatca ccgatccgcg tccgtaacgc agccgcatgc ggtccatggc ctggtaaagg
    27661 ctgatgagtt cgggcgtgtc gtcaaacagg tcgatttgct gggttccgct gatgaggtgg
    27721 ctgaaacgca cgccgataag gcggatcagc atacggcgct ggtaaaccct gcgaaacagg
    27781 tctttggtta cgggtataag cacatggtcg aacgaagtgt aagggactcg ctgctggatg
    27841 gtatgggtgt cgaagttgga gtagcggatc ttcaccgtga cgcaggaggt gagtttctgc
    27901 tttttgcgca gggtaaaggc ggttttttcg accatgcgca ccagcaggtc gttgatcatg
    27961 ttgatatcaa tggtgtcctg gtcgaaggtg gtttcggtgc tgatgctttt acgttccgag
    28021 tatggaataa cgggcgcagg atcgattccg ttggcctttt tccagattac cagcccgttg
    28081 cggccaagca ctttttcaat catttcgggg gggatgcggc tcagggtatc gatggtggca
    28141 atgcccatgg aacgcagcaa ccggaaggtc ttatcgccga tgccgggaat tttccggatg
    28201 gagagcggcg ccaggaacgg gataacgcgg tcgcggggga tttcaagctc gccgttgggt
    28261 ttggcttcgc cggtggcgat cttgcatacc gttttattca ccgaaagccc gcacgaaata
    28321 ggaaggccgg tttcatggat aatggtctgc cgcagttcgc gcgtccattt catgcagccg
    28381 aaaaagcggt ccatgccggt gatatcgagg taatgctcgt cgatgctggc tttttcatac
    28441 acgggagcct gttcggcaat aatggaagtc accatgttgg aataccgggt gtattgttcc
    28501 atgtcgccgc gcacaatgat ggcgtcgggg cagagaatgc gggccatttt catgggcatg
    28561 gccgaatgta cgccgtaatg acgggcttcg taactgcagc cggctaccac gctgcggtcg
    28621 gaaaagcccc cgacaatcac cggcttgccg ataagcttat catttattag ccgttcgacg
    28681 gatacaaaaa aggtatcaag gtcgaagtgg acgatagcct ccctccccgg cacttcgctc
    28741 ccggcatctg ccggaaaaat attttcacct tcggcgcaca tcttccaaaa aatcgtatta
    28801 cctttgatta gaaattaatc aaaaacgtga ttacaatata atcaaaattc taatacaacc
    28861 gaccgatggc taacaatttt ttcgggcaga atataaagct gttgcgcaaa aggcgcgggc
    28921 gcacacagga cgatattgcc gttgccctcc agatgaaacg ccctaccctg agcggttacg
    28981 aaaacggcgt ggccgaaccg ggaatagagt tgctgatggc tttttcggat tactacaaga
    29041 tatcggtgga taccttatta aaacaggatc tgtcggcatt gcccgaaagc cagttgtcgc
    29101 aactggaacg gggatttgat gtctttatca aaggaagcgc ccttcgggta atggccacaa
    29161 ccgtcgacag taaaaacagg gagaatatcg agcttgtgcc gcaaaaagca tccgccggct
    29221 atcgtaccgg ttatgccgat ccggaattta tacgcatgtt gcccactttc cagttgccgt
    29281 tcctgtcggc cgaacgcaaa taccgcatgt tccagatcag cggcgactcc atgcttccca
    29341 tacccgacaa atcgtggata acgggcgagt ttgtgcagaa ctggcaaaat gtccgcgacg
    29401 gcctggccta tatcatcctt accattgaag acggtattat gttcaaggtg gtagaaaacc
    29461 ggatagaaac cgaaggccgg gtacggcttc actctctgaa ccccttgtat gaaccgtatt
    29521 atgtggatgt gaaagatatc agggagatat ggcgatttgt gcattatatc agcgccgaac
    29581 tgccggagcc caacctgccg aaaaacgaat tcgcaacttc cctggcatcc ctgaaaaagg
    29641 aaatggccga tctcaaggct cagctcaaac agggaaggct ttttgaagaa tgatcggggg
    29701 tgttccgcga tttcaatgac atttgttgta aaaagcagga cggatattta ccattcgatc
    29761 acgcttttct catcgacgtt gccggtcata atccttttga cctgttgggg aaacttcctt
    29821 ccccatacat ataacgggtc gttttcctga agggaagtga gcaaatccag ggtaatctct
    29881 tcggcgaaaa cctttttccc gtttatttta tattccgatg cgtcgaagcc gtgaagcagc
    29941 aaggcgattt ttgtgaacct gccggcgtac gggccaacgg gagcttccat aataattttc
    30001 ctgcttatcg gatcgtagct gaatttttgt gtcctgaagt ttcctttttc attctcgaac
    30061 gtggtgccgt cgtcttcgta aaaggtatat tctcccggct tgtttccggc ataaacgtgc
    30121 aggaacagcg tatccgaagg cttttccgcc gtgttttgaa caatgctctg catcgggatg
    30181 aaactacctc ctttggcaaa cacaggcagt ttatccaggg gacattcgac gatgatctcc
    30241 tgttggcctt catagcgctt tccggtaaag aaatcgtacc attccccttc gggcaagtag
    30301 actttcgtga gttctttggt gctttcaaca ggcgctgcca gtaatgacgg accgaacatg
    30361 aactggttct ggtaggcata gtaccagcat ttttcttcaa aagtattggt gacggcaagg
    30421 gaacggttca cgggaatgcc gcttgtgtgc gcttcaaaga aagcggaata caggtagggg
    30481 agaagcctgt atcgcaggga tatgtaattg cgcgatatcc tttccgcttc agcgccatac
    30541 gaccagggct ccgacatttt aaaatcataa tgcttatgaa ccctgaaaaa cggcgtgaag
    30601 gtcccgatgg ttatccagcg ggtaaacagc atgttgccgg cgtccccgtt cataaaacct
    30661 cccacatcgg ttccgcagaa aggcattccg cttattccca gcgagtttac cagacggcac
    30721 cctagcagca tgtgttcatc gctcgaaaca ttgtcgccgg tccagatggc agtataacgt
    30781 tgcgccccgg aaaatgcggc acgcgtgagc accagcggcc ggctgttcat gtgtgttttc
    30841 gttccttcgt aagtgctgcg cgccatcagc aatccgtaaa cattttttcc ttcccggtag
    30901 gaagcgcctt tgccttccca gttgaacctg acgatggaag aggttttccc ggcgccccac
    30961 gacgctattt cattcatgtc gttccagaag cctgtgacgc cgtcgttaac gatttcgccg
    31021 aattcatttc cccaccattt ccgggcttca ggcatagtga aatcgggaaa gttgcaccag
    31081 ccgggccaca cctgggccgt ataatccgtt ccgtcgggat atttgatgaa catattcttt
    31141 tttacgccat cctcatatgc tttatagcct ttttcaactt ttatccccgg atcaatgatt
    31201 accgcggtgt gaatacccat accggcaagg tcttttgtca tttgttccgg atgcggaaaa
    31261 cgcaggggat gccatgtgaa aactttgtag gcatccatgt aatgaatatc gaggtacagc
    31321 acatcgagcg gaattttttt attgcggaaa gtacgtgcga tgtctaacac ctggctatcg
    31381 ggaaaatagc tccacctgct ttgctggaag ccaagccccc agattggcgg catgggcatc
    31441 cgaccggtca ggccggtata atttgcgata atccgcgaaa ccgaaccgcc cccgataaaa
    31501 taatagttca tttcgccggc atcagcgccg aaggaggaat agcggtcgtt ggaagctccg
    31561 aaattaaaat agcttttaaa ggtgttatcg aagaaaatgc cgtaggcaac gttgtgatgg
    31621 atgccgatat agaacgggaa agatgcatag atagggtcct gggattccgt ataaccgggt
    31681 gtgtcggagt tccagtttac aaatccctcg ccccggcggt cgagattgcc tgtcttttcg
    31741 cccaatccga taaatttctc tccttcctgt aatttcttga aagtcgttac ttcttcccct
    31801 acccaggtcg ttccgaaagc gccgtcatcc tcgtttatca ctgtttttcc gtcatgggtg
    31861 gtaaaagtaa aacgtacggg agtggtactt atctgcagcc ttaacgaatc ggtaatcagc
    31921 aatatctggc cttctgtttc cagaatttta acattacagg gaaccagatc cgccgttacg
    31981 gcatatgaaa aatcgccggc aggaagtttc ttattgatcc ttatccggat gatttccggc
    32041 gaatacacct cgacctgcgc tacaccgttc gtggtttcga tgaccaggga atttttgcct
    32101 tttgacaatt tcctgatttc gcccagactg ttatccggat attgctgcgg gaaagccagg
    32161 cttacaataa agaatgaaaa aatgaaggca agtgcgtttt tcatggttca tgtatttgat
    32221 tacaaaataa atatattttt cggctttccg gattccggga attaccggga tcatctctta
    32281 tttgtgatga catccggatt tctatttttt cctacctttc aaaaaaaaat taaaaaccat
    32341 acgtcatgct tcaaagaact cttatattgc taatatttag catgttaagc ttttgtgttt
    32401 tcgctcagcc aggcgggaat aatctgaatt ggtcagcaga tggcaacagt tacacggcat
    32461 tggaacgtgg aaatatcatg aaaacagaac ttccttcact caggaaaacc atgctgattg
    32521 atctggaaaa gttgttcccg caagatgctc cggaaccccg ttatgttgac gcttttgaat
    32581 ggtcgacagt aaaaccggtt gtactcctga tggtgaatac cgaaacgcag tatcataaaa
    32641 caaccggaga agtctgggtg tacgatacaa ttacaaaaaa gaccgtcatg cttggtaaat
    32701 cgttaaggcc atccggcctg atgcatgcta aattatcgcc cgacggaacc aaagtcgcat
    32761 atgtgtatca ggataaaagc aataataagg ttgtctataa tctttatgtt gaggatattt
    32821 caacgggcat tgccaggcag ctcacatttg atacccgtga ccgggtcatc aacggcactt
    32881 tcgactgggt ttatgccgaa gaactttcct gttatgatgg attccgctgg agccccgacg
    32941 gaacccggat cgcatactgg aatattgatg cttcgcagat acgtaattac ctgatgctta
    33001 acacgacaga ttcaacttat tcgtttactg ttccggttga atacccaaaa gtgggggaag
    33061 atccgtcgcc ggcaaaaatc ggagtagtag atattgaaag tgcaaaaacc atctggatgg
    33121 atattcccgg tgatccgcgt cagcattacc ttacccggat ggaatgggca aacagccagg
    33181 aattgatcct tcagcagctt accaggaaac agaacgaatc gaagctcttt atagccaaat
    33241 catcggacgg cttctgccgc ctgttgtctt ctgaaaccga tgcaacctgg atcgatgttg
    33301 aatcggcatg gaacgacggc gataattcag ggtggaactg gattgaaaaa aaacaggcgt
    33361 ttatctggtg cagcgaaaag gacgggtggc gacatttgta ccgtgtcggg atggatggca
    33421 gggaaacgtt gctcactccg ggggactttg atgtgatgaa gatatactgc attgatgaag
    33481 gtaagaaggt tgtttatttt gccgcttcgc cgataaatca tacgcagcaa tatctttaca
    33541 gcattcctct tggcggggga aaggcaaagc ttctgacacc cgaagggctt cccggaacgc
    33601 attcgtacat gatatcgccg aatggaaaat gggcgcgtca ttctttcaac agccatgttt
    33661 ttatgcctgt tgccgagtgg gtttcgttgc ctgcccataa accgcttgat gagggtaaaa
    33721 gcatttcgaa aaacctgaaa gaaaatccac tggcaaaaca gattgagttt ttccaggtta
    33781 ctaccggaga tggtatcaca cttgacgcat ggatggttaa accggccgat tttgattccg
    33841 cgaagaagta cccggtgata ttttatgtat acggcgaacc ggcatcctca acggttctcg
    33901 ataacgctta tatcggccgc agttcggttt tcggggataa agtgtatgac cgcggatacc
    33961 tgttggtatc ggttgataac cggggtacgc caatgccaag gggcagggaa tggcgtaaag
    34021 ccatctaccg caatatcggt attaacaata tccgtgattt ggcattgggc gctaaagaaa
    34081 tcctgaaacg gccctattgc gataccagcc gggttgctgt tacgggttgg agcggcgggg
    34141 gatcatccac gctgaacctc atgttccagt atcctgaaat tttcaagacc ggcgtttctg
    34201 tagcctcggt ggccaaccaa ctggcttatg acaacatcta ccaggaacgt tatatgggtg
    34261 ttccatggga aacgcgtgag gattttctga aaggctctcc ctatacccat gcaaaaaatc
    34321 tgaaaggtaa cctgttgtat attcacggca cgggcgacga taatgttcat tatgccaatg
    34381 ccgaaaaact gatcaatgag ttgatccggt acaacaaacc attccagatg atggcgtatc
    34441 cgatgcgctc gcatggcatt tatgaagggg aaggcacccg cgaccatctg aaaaccacat
    34501 gcctgaagtt cttactcgaa aactgtcctc cgggaggaag gtagatcata cccggtacag
    34561 aaatgcaggg atgacgttct ctttttttgg ggaagcatcg ccttataatg acttgtaaaa
    34621 gaggccgcga tcatcggcct ctttttatgt cttgaatgca gaaacaaact cacgaaggtt
    34681 agaggataaa tttcccggat aggatttgtc cggcagagta tgccctgatg acgtacatac
    34741 cctgtgaaag cccgtcaagg cggataaggt aagtttgcgg gaaattctgt tcttcggata
    34801 cactgactct ccggccggcc atatcaaata tttccacttt gcggtacgag gctttatgtc
    34861 cggtaagagt gagcgttttt gcgttgtgat ccacgatgaa ggataaattg ggagccgccg
    34921 ggttttcatc aaccgatgcg ccctggttcc aaccggccag tcgggccatc atccaccaga
    34981 aagcattccc tttcagcacg cagttgagtt tggcttcgtt gggtgaatcg ctgtgggcac
    35041 attcgccgca tgccagcaga tccgtaacat attgatgtcc ggaatgggtt gctacccatt
    35101 ccacgcccca gttagggccg ccccagggat cttcgcatat aggatcataa ttcagcccgt
    35161 cgagggcgca ccactccata tagttttgcg tgttgcccgg agaataactt tcaatatcgg
    35221 caaagtcgaa gagcgttttc tgattttcag cacaataatt cctgatcagg ttgttcatgt
    35281 ggttcaggtt tccttccgaa ccggagccat ccagatgtcc ggtcatataa acaaatttta
    35341 cgtccggata ctgtatttcc aggtaattca ttccgttcag ataggcatag atgccggccg
    35401 aatcgttatc gctgacgcct ccgcaccatg accacatgac tacgttgcga tcgcatgccg
    35461 ggtcattgag cagcgcgatg gtagcggttt gccaggcaag atcgccataa tgccccaggt
    35521 cggatgcacc gggaatgccg taatcgttca ggaaaacgtc tgtgaccagt ccgtttcccg
    35581 aggaagtgaa tgaaaattcc ggaccgtggg tggaggctat cgcgttgatc ccgctcacca
    35641 gttggctgcc atgtgatgta tgctggtaag tgactttcag gtttgctttc gcggaatcga
    35701 gccatgctgc cggcactgct gcgaggtctg tacaggtatg gttgatgagg attgcctgac
    35761 tatagacctg acataatgca gacaaaaaga ggaaagccgt aatgcataaa tgtttcatcg
    35821 attcctgttt taacgcttgt aaatcctgac tgaataaact gatgaagaac tttaattaga
    35881 aaagggcagg ttaaccctat tgtaaagata cgaaatcagg gacgttattt ataaaggttc
    35941 ttttaagagg gtaccggaaa aaatcataca agtcagatag atgattcctg agtgcttaag
    36001 ggcatgttgt atcgcatatc ctgcggcgtt tatccatcag ggacaacctc ttttttcctg
    36061 ttttaacgat gcaatatccg ctgccggttc gtcgcattat tattataaca gggagctatt
    36121 tccggccggg gttcagcagg ttcaccctga ctacccaatc cttttttacc ttggaagata
    36181 tattcaggtc caaaacgttt ttatctatcg gctgcaacga tgactggccc gtttttgtat
    36241 caatggccag ggcgagatcc ggggtaccgt tgttgtcaaa atcaatggta tcgctgatga
    36301 aaggcggtaa tgaatcggcc ggagaaaacc tgacctgcac aaatcctttc ctgctttcgc
    36361 cgatcagcgc atcgaaaaca ggtttattca cggttatttt aaggttatta tgctcgtaaa
    36421 cccgaccgat ttcacctccg gaatgcctgg ggttaatttt catgaaaggc agtctggcca
    36481 gcatttccga aaatcctgtg cttcgcacga atgtgacagg gatcaggatc atacaaagga
    36541 ctgcccacgt atatatgata atgtttctga tggttttcat aacagaatgt tttaaaagag
    36601 actgccggtt gtgaataatc ggatcaggag cgtgatgccg tactgcatgg ttccgccggt
    36661 gaaaatcacc agcagtgtga aagcagggta aaggaacagc atgcgtgctg ttgtcagttt
    36721 cttatcggac ggcgttcctt ttaagtgata atgaatggct tgccgggaac aactgtcgat
    36781 acacttcccg catttgcagc aggtcatcga aggctttccg cgggctactt cttctttgtt
    36841 aagcgagagt gtcggacata tagtgacgca tttcaaacag gagttgcatt tttccttgtc
    36901 gatttttaca tcaaacacgt tgattttatt ggtaagcgag caaagggcgc ccatggggca
    36961 gaaggtggcg cattggatgc gttttttggt cagcagcgga agaatgatca ccaaggccgc
    37021 aaagagggaa acaaacacga ttgttttcag cagtgtttca acggaggtca ccttttcgaa
    37081 ttcggtgacg gttttaaagg gacacagcca gttgcaatac tggggcgtaa gggtcagcgc
    37141 cgcccacagg gcaaccagta tcagcacggc aaaggctgcc caccggaaga ccgggttaaa
    37201 tttcctgatg acgggtttct tcaggatgcg cgacgtcccg tcgtcccagc ccccgtaaaa
    37261 gcagccccag ctacaaaatc cccttcccag cgcaaggctg atgccaagcc agatgacgat
    37321 catcaaggag atggcggcaa acccttcgag gatgctcccc gggaaaataa tggtttgggt
    37381 aagcgccgcc gggatgatga tcatcgtaat gacgatatgg caaaaaggaa tttcacatgc
    37441 caggatattc tcatccgaaa acgacatatt ccccctggcc ctgaccaggt taacaataaa
    37501 gacgaaggca aaaaacagcg ccagcactat gaaccctgca gccctgtacc ggtcggtttt
    37561 tccggtatac aacatccgga agaacaggta attgaaaaaa acataggtga tgataaatcc
    37621 gatgacctgg ccggttcctg aaaaatcggg ttgcccgccc gtgatgaata cgaaagtgat
    37681 cagaagcatg ggcaggagga aaagtaccga tttgccgatt tttcccgttg tagaataggc
    37741 tgaaggagtt ttcattcttt aattatttgt gaaccagcat aattaaaccc aggtaaaaat
    37801 aataaaatcc cgccaggatt gaagcaaact ttccgataat ctgcaatact ttttgccgcc
    37861 tgacgaatcc tatgagcggc aagggaatga agtaaacaga tgtccccaga aaaaaccaga
    37921 aaaacagcat taaactcccg gaaatgtttc cggttgcaaa agcctttgta aatgccatca
    37981 tgaaaggagg acaaaggttg aagcccgtca ggactccccc gagaaaaggc acccatgctg
    38041 tcaggtccgg gccgaaatgt attttaacat ttttaccggc actgcccagg catatttccc
    38101 tgaaacgatg aaagccgtaa ataatcatca ggagggccag caatatataa acgatggcca
    38161 gcgccctagt aaataatgct gatttttgaa agaatgtcat tccgatcagt ccggcaatca
    38221 atcccgtggt cagatacgca atgagccttc cgcccagaaa aagcacgagt gaaaggtaat
    38281 ttctttttac ccttgtgctt tcagccagga tgtatggaat cagaacaggc ccgcagtatg
    38341 ccaggcaaac cacgcccgtg ctgaggccta agatgaatcc ttcggatgag agcatggttc
    38401 gtggtattag gtttcataat tacaggcttc ctgccgccgg acaggaacct gaaggagcct
    38461 gaagaacaaa ggtaagggaa tgagaaacag aaatcaattt ctgtatgttt tcgtttcgtc
    38521 cggcagcata cattaaaagc gcattgtaaa aaccgtttcc gattccggca ccgaaatcac
    38581 gtcaagactg ccgccatgta accgcataat ctgccgcgat atactcagcc cgattccgga
    38641 accgtcgtct ttggtcgaaa agaaaggaat aaaaatctgt tcaatcagtt ccggttttat
    38701 acccggcccg ttgtcggtta cagcaatttc cggcctgtta tattgcgata cctgagctgt
    38761 caaccggata gctaaacccg gatacccttc acctgcttca aaagcatttt ttaccaggtt
    38821 aacaatcacc tgcgagatca tcccctgatc ggcaaaaatc tccagatcgg gaggcgtaat
    38881 cttttgcatg agcgggatgt ccgcatttcc aggaaccgac ctgaacaatt ctgaaattct
    38941 tgcaaaaagc tcttcaatgc taaacaatgt tttctcgggt ttcggcagcc tggtcagctt
    39001 tctgtatgat tcaacgaagc ggataagccc ttgtccctga tcgccgatta ctttcaggcc
    39061 tttgacgagc gtattgacgg tctcctgtcc aagttcggaa ggcttcaggt taaccgagtc
    39121 ccttatcagg taatgcgaca agctttcgga tagcgtcgtt atgggggtga tggtattcat
    39181 gatctcatgc atcattaccc tgatcagttt gatccaggca tccgtttctt tttcgtccag
    39241 ttcatttctg atatcctggg ccgaaacgat gtaataagca tcgtcggttg attcgaaata
    39301 actggctttc agaagcaact gaacaatgac cggcccctgt tgaaggctta caagcttttg
    39361 ttcatcgggt ttcagggctt cgattgtttt atagagttgt tcattcaccc gctcaagttg
    39421 cctgatatgg gtgaaagttt cgagcccgaa catctttttc accgatgaat tgcagtgaac
    39481 tatgaatcct ttccggttga aagccatgat gccggtagcg gcatgttcaa gcagtatcct
    39541 caaatactga tcctgttgct ggctggcaac acgcacttcc tgaatgccgg catttagttc
    39601 gttgagctta tcgctgagtt tgtcgagcgt cctatcgccg gtatcattgt tgaaccggac
    39661 cgtaaaatcg tcgttcctga ctgattcaaa gaaataaagg agtctcctgt cggttttatg
    39721 gaggtatttt attgtgttga agaataccag gcctgtgaaa agcgacaagg cgcacagcaa
    39781 taaaacagaa ccgtgggcca tccaggtgca gcacaatgca aatccggcca gtaccagtgt
    39841 tatcaggcgg atgaatatct gcatgtaata atccctgatg atcataaacc gtatttttta
    39901 atcttgttat acaaagtctg tcgggtaaca cccagttgtc cggcagtagc gctcaggttg
    39961 ccgttgtttt tctgcatcac cgtccggatg aagtgttttt ccatttcttc gagtgaagag
    40021 gctgcttcat cagggcggaa tacagaagtt tgttgcagga aaaaatcttc aggtttaaga
    40081 atgttgctgt cactgagtat cacagccttt tcgatggtat ggtccagttc ccttacattc
    40141 ccgggccatg aatattttaa taatttttcc ctggcgttac tgttgatttt caggtttgct
    40201 ttttcatatt tcgaggcgta tttcctgatg aaatactcag ccagcaggat aatgtcttcg
    40261 tctctttcac gaagcggagg aacctcaatc tggatggtgt tgatccggta aagaaggtcc
    40321 tcgcgaaaca acccgtcgga gaccatctgc tgaaggtttt tattcgtggc gctgatgagc
    40381 ctgatatcaa ctgcaatagg cctgtcggag ccgacacgca ttacttcccg gttttgcaat
    40441 gccgagagca gttttgcctg aagatgtaag gagaggttgc cgatttcatc caggaacagg
    40501 gtgccgcggt tagcggtttc aaatttcccg gccctgtttt cgcgggcatc ggtgaaagag
    40561 ccttttacat gaccaaataa ttcactctca aaaagggtct cacttacggc gcccatatca
    40621 acattcacca acaattcatt gcttcgctcg gatagcctgt gtatttcctt ggctatcaat
    40681 tcttttccgg tcccgttttc gccggttatg agaatattgg cattggtgcg cgctaccttc
    40741 ctgactatat tcagcaccag cattagtttt ggcgaattgc ccatgatcgc cggctgcgtt
    40801 ttgttgattt cctgcttcag gaaattctct ttttgcttca gttgcataac ttcctgtctc
    40861 gaatggttca attgcacggc agctttcagg gttgccagca attttgaatt ctcccatggc
    40921 ttcaggataa aatccgtagc ccctcttttt aaggcttcta cagcaacttc cacatcttta
    40981 taagcagtta tcaggacaac ggagatatcg ggattggcct ccaggatgcg tccaagccag
    41041 tatagccctt cgtttcccga attatttcca gccttaaaat tcatatcgag aatcaccagg
    41101 ttataattgc aattcctcaa ttcggtcagg atcaggttgg ggttggtgat tcccctgaca
    41161 tggtcaaatt cgggtatcag caatatttcc agcgctgtga caatgctctt gttgtcatca
    41221 acgatcaata cgcggctttt gggcatgctg tttttttttg taaaaatata ccatgcaatt
    41281 atcagcctga taaaagtgtt aaaatattat acataaattg taaataaatt ttacaattga
    41341 gggttggcga ggctgtagat aaaaagctct ttgtcagtgt aataggcgat atggtaagat
    41401 attggatagt cagaaagcaa gactattcat gatatgcgat tcggattgat ctttttgatc
    41461 ctcatggtgg caagcagcag cctgcaggcc caggaaagac tgaatttgtg cagatgcatc
    41521 agatatgcga ttgataataa tctgcagata aaaaatatag cgattagtaa gaaattatct
    41581 caggaaaggc tcagccaaag cagacgcgac aggctgccgg atgctggctt ttactccggc
    41641 gccggaatca gttttggcaa atcaattgat cccaacacca acgatatcat ctctgccggt
    41701 ttctttaaca gcacttataa tttcagcagt tccatcacct tgttcgatgg ctttcgtatg
    41761 cagaagaaga ctgattttga acgtttcagg ttgaatgccg aagaatttcg cctgcaaaat
    41821 gcagaagatg aactcgcttt tgatgtgatg acctgctatt acgatattca atattataaa
    41881 ggactgacaa agatagccag ggatcaggcg gaagcctctg cgctatggct aaagatgatt
    41941 gaacgcaagg ctgaggcagg gctgaaatcg gtaagcgaca tagcggaagt cagggctaac
    42001 ctccagttag aagaagtaca caaggtacag gccgaaaaca agctgaacga agcaatgctg
    42061 aatctgcaaa agctcatgaa tgttgaaaaa cctattgata cggcagacat tgaagaggct
    42121 ccggagagct ttccggattc aaccgggata atgctatctg gcatcgatcc ggatataatc
    42181 ttcaacagtt tcctgttgcg gtcgccttta tatctttccg aagaggccaa ctataaagca
    42241 gaaacgaaga gcctttcaat cagccgatca aatttttatc cggcaatttc cggcggctgg
    42301 tcgatagggt ccggttatta tgaaaccaat accactcccg acggggatgt aatatcattt
    42361 gcacggcaac tgaaagacaa tcgcaaccag taccttggcg tcagcctgaa ttttccgctg
    42421 tttacgcgga tggcaaaccg gtcggctgta tcggttgcca ggctgaatgc ggagcaggcc
    42481 cgcaacagca tggacaatac caggcaaaat ttgtacttcg gggtttgcaa agccgtcaac
    42541 gatttgaaag ccttacaaaa ggaatccctt cagtatgtca ggcaacagga tgccgatagc
    42601 ctggcattgg ccatggcgga gaaaaagttt gctcagggat tgatcagcgt tgttgattat
    42661 tatgtggcta aaaaccgctt tgccaattca caaagccagg tgttgcggag cgccctgcaa
    42721 cttgatatgc aggcaaggct gattgcgttt tatgagggag acaggttttg ggagtaaata
    42781 tggagggtaa aaggcaagga gaacggagaa gggaaaactt tacctgccgg tgtatggcat
    42841 tgtgtgagag gcaggcaggc agg
//
LOCUS       DAOPYV010000023        40729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_838_length_40729_cov_11.910950, whole genome shotgun sequence.
ACCESSION   DAOPYV010000023 DAOPYV010000000
VERSION     DAOPYV010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 40729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40729
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_838_length_40729_cov_11.910950"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1322)
                     /locus_tag="PKI35_04565"
     CDS             complement(6..1322)
                     /locus_tag="PKI35_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNX43689.1"
                     /translation="MQDHEHKTNRIVRQLKERECTKPVSLRKKAVSHEVPKLNDKRRL
                     DEKIDVSDFNEILSIDTGNLICEAEPGVTFIDLVKATLKHGLVPIIVPELKTITIGGA
                     VAGCSIESMSYKYGGFHDTCLEYEIITATGEVLTCTPDNENSLLFQMVHGAFGTLGII
                     SKLKFRLIPALPFVKVTYESFDNPGDYQSAIWKHYQEQDIDFMDGIIHSETSYVLSTA
                     SFTDRAPYTHRYDWLRVYYESTGKRKEDYLKTPDYFFRYDRGVTNVTPASFPARLIFG
                     KFMNSARVLKLVEKFRKLIPPEKIPVTIDLFIPFSKVGRFMDWYQKELRHFPLWCVPY
                     KLVRKYEWLSDDFRRRIDDELFLDIAIYGMKKTGSKNYYRMIEEELTDIGGIKTLISN
                     NYYTEQEFWKIWNKENYGVVKRRTDPDNIFRDLYTKTCKTMMGLES"
     gene            complement(1461..2597)
                     /locus_tag="PKI35_04570"
     CDS             complement(1461..2597)
                     /locus_tag="PKI35_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43690.1"
                     /translation="MNKLPVIFAGLLFSAITAFGQEPIDVTEQTIKLGGMQEEELYYG
                     FAAGDKIIFSFRETGGQEIKEVEITEYPGNSRFSAYKTSHVDNKTITVSTQGVYIFRI
                     KNSALAGRVCLIKIQRVPVSGDTRNFNTAVTWEMRPDTTYSTCTREAVIGYDTSYIQK
                     TKKELVKTEISEDIIADKTERVHSRNNLQHKNYSILPVSLPQNEVTPFMTKTVRSWAY
                     WIGVGKEASEAWLKNVNIIKNIASGASVILGGSPLAGIAIGTLATLAMPSMGEDVAYW
                     FIPDYANARLFMEDKDFVQFDSGKGIAAYGKNTDRTQGTFYIGLLNDNQVQGIDVNVK
                     ISVILETKYYEDKPYLEISLTPRYGKKTFSEPVIRTVKVPVAGK"
     gene            3091..4986
                     /locus_tag="PKI35_04575"
     CDS             3091..4986
                     /locus_tag="PKI35_04575"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNX43691.1"
                     /translation="MDKSLKNSIIARSHERSRKFGVEPDRIFPRHIIRGADLKRQFAV
                     NEELINVSDPIINQLLSSVNETGFIIVLTDAVGCILIIKGGERTLEAAAKLNMIPGAV
                     MSEESIGTNSMGTAISENAPVQITAKEHFISVYHQWTCSAAPIHFEGKIIGTLNITGS
                     ADQVHPHTLGLVQAAVDAIENKLITNKVQSELEASNQYAFAMMNNLAYGVFAIDLNDE
                     ILWVNDSACRIINIRRTKLLYKPIKEFVPAWEKIADTLLNDKPFIDQECKLAVKDLKE
                     RFLFNSYLIKSENKEILGYLLTFRQYSNMLNLVNRYTGFQAGYTFDDIIAQSRSMSEL
                     VNYARTVANSPSTILITGESGTGKEVFAQALHNAGERSEAAFVAVNCGAISPTLIESE
                     LFGYEEGAFTGARKGGRPGKFELASGGTIFLDEIGEMPADMQVKLLRVLQEGEVTRLG
                     GDSSTKIDVRIIAATNKNLEAEVEARRFRLDLFYRLNVIPIHIPALRDRREDIIPLWQ
                     HFIATKAAKLNKPVPELTKDIMDKLMSYSWPGNIRELENFVEKLVNFGGVPELADTPA
                     ETDQNGAEVSAAVKKINSLEETEKEAICDAINALGGNISKVAKQLGVGRNTLYVKMKK
                     YDIKLRE"
     gene            5355..6521
                     /locus_tag="PKI35_04580"
     CDS             5355..6521
                     /locus_tag="PKI35_04580"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNX43692.1"
                     /translation="MNRFTLPRDLYYGKGSMEVLKTLKGKKATVVVGGGSMKRFGFLD
                     KVENYLKEAGMKIQVIDGVEPDPSVETVMKGAKLMQQFQPDWIVSIGGGSPIDAAKAM
                     WVFYEHPESKFEDIAKPFNIPPLRNKARFVAIPSTSGTATEVTAFSVITDYSVGIKYP
                     LADFEITPDIAILDPEIAYTMPAKLTAYTGIDALTHATEAITASQASDFSDPLALKAI
                     VMIKDNLEESYAGNVTARDNMHVAQCLAGMAFSNALLGICHSMAHKTGAIFDIPHGCA
                     NGIYLPYVIRFNRKNAEARYAMIARFIGLKGENDKELCDAYINYIEALNQKLGIPATL
                     KEYGVGENLFREKLDKISENAALDACTFSNPRPATPDDFRKILTAAFYGTKENF"
     gene            6582..6773
                     /locus_tag="PKI35_04585"
     CDS             6582..6773
                     /locus_tag="PKI35_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43693.1"
                     /translation="MIMFEITENTYQVPDIRPVQVKDPARIKTDNPVLSSIIIVVAIL
                     KSVIGVGMPTAPANNGFTL"
     gene            6864..7121
                     /locus_tag="PKI35_04590"
     CDS             6864..7121
                     /locus_tag="PKI35_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNX43694.1"
                     /translation="MNNRMKIVICLGSSCYNRGNQYVLEAIKKYLNEHNLKDKVDFRG
                     QLCSGNCAQGPVLHINGKTYKEIDENVAIRLLDNLFAANKN"
     gene            7127..8848
                     /locus_tag="PKI35_04595"
     CDS             7127..8848
                     /locus_tag="PKI35_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNX43695.1"
                     /translation="MLQAIYTERNNCQDCYKCIRQCPVKAIKIEDHSASVISSLCLYC
                     GRCVQVCPVNAKKVRNDLNAAKFHLSASSRVVLSLAPSYKSEFTDYTLPQLLEGLYGL
                     GFFKVSETALGAELVSKATKKWLDEQPDGIYYSSCCPSIVRLLQKYYPEQLRRLAPVD
                     SPMQAHARFIRKMYGEHTCVIFAGPCIAKKKEIEDYQGGADLAITFAELREWLKGEGL
                     TPDFLSGSGGYSFAPYNANNGSIYPVDGGMISTMKQNVGIADISFMSFSGLQNVKGII
                     EQLPEKYEGKLFIELMVCEGGCINGPVVSHPGSTVGKRVLTLKQPLNNGVGIRDVEDK
                     GMDLFADYSGTKPVTRCVHTEKEIQDALKTVGKVTDKDELNCGGCGYESCRRFAEAML
                     DGKAERNMCVSYMRKVAQNKATVLLQKMPYGVVLVDDKLKIIESNQYFARMLGEEAMN
                     IYSVKPGMEGADLRKLTPQYKLFANFLEKGLDEFNKDIRMDDGLYHLSIFSIQQFRIV
                     CGIIHKLDQQHFSKNALTERLHKVISENLATAQKAAFILGENASRTETMLHNIIDSYQ
                     SDEDGNG"
     gene            8835..9995
                     /locus_tag="PKI35_04600"
     CDS             8835..9995
                     /locus_tag="PKI35_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNX43696.1"
                     /translation="MATADYFIEIAHRNIQKAGQEVCGDVFLSRKLYDEGRTISVLSD
                     GLGSGIKANILATLTASMALNFTVEHQPPERTSQAIRDALPIDQDRKISYATFSIVDI
                     EYDNETSIVEYGNPPFIVFRGAAPYKGIIRKDFNADIKKCNFCAQREDRIVLFSDGVS
                     QSGIGRIEMPFGWDSENIENYISGILEKNPGISAEELAQKIIGRAYMNDSMHAQDDIS
                     CIVIYFRKPRKLLICSGPPYKKEKDRLFADTIECFDGNKIICGGTTAQIIGRELGLEI
                     ETDLFSGDNQLPPVSYMKGIDLVTEGILTIGKVAECLESGEMLPEKNPAGKIIRYILA
                     NDHIRFLVGTKINEAHQDPNLPVELEIRRNVIKKIAQILKEKYLKEVEIQFI"
     gene            10016..10285
                     /locus_tag="PKI35_04605"
     CDS             10016..10285
                     /locus_tag="PKI35_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNX43697.1"
                     /translation="MSAEKKVVVKICTGTLCHVMGGSELPELAGHLPESIKPKVEIKG
                     MTCGGFCKDGNRKPPFVTINDVLMEQASIEKIVSNLEKAISDDAE"
     gene            10272..11690
                     /locus_tag="PKI35_04610"
     CDS             10272..11690
                     /locus_tag="PKI35_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric [FeFe] hydrogenase"
                     /protein_id="HNX43698.1"
                     /translation="MTPNNLALLTRRELLVHLCRMLDQGTILDEIDRLPVLMRPRHNQ
                     PIRCCVHKDRAILKYKLMALLAFNVDDETDELCQLHEYAATALKREKLTPVAMTVVDE
                     ACSSCNQGSYEITNLCRGCDARPCEVNCPKKAVSFADGKARINHDSCINCGKCMKECP
                     FHAIVYQPVPCEESCPVGAIEKDDTGIERINFDKCIFCGKCIAACPYGAIVEKTHLVD
                     VFRAWKSGRKVVALVAPAIAGQFRQDLGKIYSSILSAGFHDIVEVAEGADLTIANESA
                     EWKERMAAGCQFMTTSCCPAFKELVKKHIPEMSDKVSSTPSPLCYAAEIARKRFPDAV
                     TVFIGPCIAKRVESYYLGSVDFMMSFEELGALLVAKGIEVADLESHPRPERSSAQAYH
                     FAYSGGVAGSIVASAGSSVNAEVINGIDKKSITLLKQMAKGKTDKNFVEVMCCEGGCL
                     GGVNTLIRSPQAVEIFKKNLEA"
     gene            11742..12257
                     /locus_tag="PKI35_04615"
     CDS             11742..12257
                     /locus_tag="PKI35_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43699.1"
                     /translation="MPEGYGLIPEVMDLAKNQAAESGGSFRALNSMDEAFRDADIIYP
                     KSWAPYHVMGCRTELLRTGDLKGLKELEKECLSNNARFREWECTGKKLKLTREGKALY
                     LHCLPADISGISCREGELSAAVFDLYKTPLYKQAGFKPYVVAAMMLANRFEDPGEILK
                     QLLENNRKWML"
     gene            complement(12377..12514)
                     /locus_tag="PKI35_04620"
     CDS             complement(12377..12514)
                     /locus_tag="PKI35_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43700.1"
                     /translation="MKKLKVVDSIVYGLVNIRTDGNKILCATLNEGKNKWEINTFRSR
                     F"
     gene            complement(12912..14588)
                     /locus_tag="PKI35_04625"
     CDS             complement(12912..14588)
                     /locus_tag="PKI35_04625"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX43701.1"
                     /translation="MKIVFTSFTALLITGMFFFNRVAVGQEVLYSNHFDNPAGQLPPG
                     WTLNGAQAPWSVSATAMAGGEAPELFLGYSFASGLSRLISPEINIDGYHELCLRFKQY
                     LFNYEMDYGEIIGLDVTYDGGATWEVLMEKPVGMLNIPQDEFSWYFTAPENAEKLQIA
                     FRFDGNNYAINFWAIDDLFVESPVENDLLTGNFSGNTTPEAGEQQLYFLEVINGGKLA
                     QQDYTVKLYSADGAQLSSEAGQPIAFGEKMYHLLWWTPSTDNIGSMDIYAKIEFAQDE
                     NQDNNRTVDLPVYVQPAGIESTQIGNGSCPLNSLPCNFFNLHSFTQSLYMPEEVGVSG
                     YPITGLQYTCQFDNDLEDVPVQILMGETSLSSLADNWADPATLTPVFNGTINFAKGFN
                     TIYIPLDNPYTYHGGNLVVQTVKSYSEMVLGAVFISSIDTGASRSRISERDDLPFDPM
                     SLPEFGYSSDYYPNITLFYSTGTTMVSPLQATPVVSAYPNPVSSRLFVQSVEPMTEVR
                     ITDLSGRDILREKAAGLEHTVNVENLIPGMYMLQIITEAGTATRKIQVIR"
     gene            14960..16762
                     /locus_tag="PKI35_04630"
     CDS             14960..16762
                     /locus_tag="PKI35_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNX43702.1"
                     /translation="MIEEIHQIIYENPSQARTLAQQLLKDRYKDNPAAKLRLLKYIGS
                     SYTLETRYPEAINYYNQALTIAKEIKLYLEIAHINNNLGMIYNETGNYKTAYLYLSEA
                     LNNYNLAGVPEKMAGTYNNIGLIYLNLKNYNKALAYFNKALDSTGKQKDSILIISVLN
                     NIALCHITGTNPSLALDYLSRAIELSQRINNKYGLCISYQLQGNYYLAHEQPERALEA
                     FKLSTDIARETKLSYQLAVARLGTAGALNRLNQTAKALTIAAEVLQMAGDQNSLVLKS
                     DAHEVLSDIYEKTGDYKSSLFHYREHIKAQQEVINQAVVHQVYDVELDYLSQRNKMQL
                     LELEKKELAISKKNNLLFFISLTFILLLIGLYLLYLNHRHRQNVKLQKTVIELSEKKA
                     NAVLEAEIQERKRIGSELHDSLGHLLSLAGLNASVLHNRKELTEEKRKLLLESLMKSI
                     DDAFNEVRTISHNLAPSLLSERGLKGALKNISDRVNQSTRLSMSFDTFGLSDKLDNLI
                     ENTLFRTIQEIVNNTIKHAEAGSLFIQITQGDHEISLIAEDDGKGFNFEEIKQHSSYG
                     LAHMKSRIENLHGTMFIDSNPGRGTIISILIPLQ"
     gene            16770..17447
                     /locus_tag="PKI35_04635"
     CDS             16770..17447
                     /locus_tag="PKI35_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX43703.1"
                     /translation="MSENPIRVLIVDDHQLMIEGLKSLLEGEEDIAFVAGANSMAETL
                     AFLEKNVIDVILMDINMPDGSGIEATEKVRALYPSVKIIALTMHDDISIISKMIKAGA
                     SGYVLKCTNMNEVTEALRIVHKNGKYLSINTQNIIMENLGSTPEQTVNKGDTRPTLSS
                     REIEVLNLVAREYSNEQIAEKLFISERTVEAHRRNIFIKTKTKSIVGLMKYAIRNGLI
                     EPENEIR"
     gene            17649..18239
                     /locus_tag="PKI35_04640"
     CDS             17649..18239
                     /locus_tag="PKI35_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX43704.1"
                     /translation="MAENLPKEERVQQIVEAAVAEFLEKGYAGASMEAIATRAGISKG
                     GLYHHFRNKDEVLVFANQVFMEPVEALMQELVQSTSVAKGLELFILHYINHWITHRKE
                     LEFYFLVMDKAFRNPNLLAAFGDYAAQMLTFMESVYQAGINSGEFGNIDPHKMAITFL
                     SAIDGSLAYIMLNEAFEPVSVIEALQETLVIKHKSN"
     gene            18365..19450
                     /locus_tag="PKI35_04645"
     CDS             18365..19450
                     /locus_tag="PKI35_04645"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HNX43705.1"
                     /translation="MIQRYDNLLMIYYSGTGNSKRVSEWIAETAAEKGMKTCVASFRD
                     FDKEMISGFTGRTLIGFFSATHGFNMPHSMLKFLFRFRELRGSDIFIGNTRAGMKLSK
                     FFMPGLSGLALYFPALVMLLKGYQIRSMYPVDLPSNWISLHPGLRPKVIDSMFVHYRE
                     LTRRYAEKVLSGRRVFLKAFILLPLDILVAPLAVGYYIVGRFIIAKTFITNEKCDNCG
                     LCMKQCTTGSIVLSNGRPFWKLSCESCMKCMNYCPKRAIETAHSFFFILLFALILFIN
                     PFLSEKVVAFVNRHLSGSGVAYEVLDFIVEWGVAIMLYVMGYRLMHWLSQYRFFNRLI
                     TVTSLTHWRFWRRYRAPGKILKPGLVK"
     gene            19646..20749
                     /gene="hydE"
                     /locus_tag="PKI35_04650"
     CDS             19646..20749
                     /gene="hydE"
                     /locus_tag="PKI35_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HNX43706.1"
                     /translation="MNQTEDLFSSEPVSKQKLISLLSVTGLRQAELFEHAARVRSKSV
                     GDIVYFRGLIEFSNKCHKNCYYCGIRAGNTRVVRYDLTDQEILDAAKFALDNKYGSIV
                     LQSGEREDNRFTRRIDRLVQQIVRDTSGRLRITLSCGEQKPDVYRRWFESGAKRYLMR
                     IETSNPVLYSKLHPDDGLHSYKRRMECLQAIKDIGYQSGTGVMIGLPYQTLDDLAGDL
                     LFMKEYDVDMVGMGPYIEHAETPLYHLKDSLLPLRERFDLTLKMISVLRLLMNNVNIA
                     ATTALQSIDKMGREKALKAGANVLMPNITPGKFRDSYKLYDNKPCLDENPEDCQSCLE
                     WRVALTGNTIAYDQWGDSRHFLDRNPKEEAPSA"
     gene            complement(20746..21159)
                     /locus_tag="PKI35_04655"
     CDS             complement(20746..21159)
                     /locus_tag="PKI35_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNX43707.1"
                     /translation="MLTQKTRYSMLALARLAREYGKGPLMINEIAKSELIPKRFLESI
                     LLDLKKNGYLGSKLGKNGGYYLIKNPNEISLLEIIRLFEGSIAFLACTSEKYYRPCEH
                     CKDEETCEIRHTFSDIKSYIFNRLKDTSLSMLARI"
     gene            complement(21162..21815)
                     /locus_tag="PKI35_04660"
     CDS             complement(21162..21815)
                     /locus_tag="PKI35_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX43708.1"
                     /translation="MKKISSQFISPQDFDRIDTACIKLRFKKGETILKQGNIPSHIVF
                     LEKGLVKFNFEDESGKNIILTVVSAPRILGGANLFYKDNNLFSIIAVEECEAVLINSN
                     VLLSVMSKNARFSIALFQIACQMFKSSIMNFISLASKQKEGRIADIILFLVNEVYHRN
                     EFNLSFTRKELAEFAGCSAENLIMTLSKWQNEGVIQITGRQLEILDMERLNLISKIG"
     gene            21994..22824
                     /locus_tag="PKI35_04665"
     CDS             21994..22824
                     /locus_tag="PKI35_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX43709.1"
                     /translation="MEINTVKVIGGRGKDGQPEKVSDFELKMGDIVSIVGPTGCGKTT
                     LINDIELFANNNTPSERQVLINGEPIPEEFSFDPSKHPIALISQHTNFLSDLPVGEFL
                     KIHATIRGANETDQMISETLAFANQLTGEAIIRETAMTELSGGQTRSLLIADAVIIGN
                     SPIILLDEIENAGIHRTKALKLLKRYRKIFVFVTHDPRIALLSDFRIIMEHGAMQQII
                     VTDTEEKVAAIGLKKIDDVMLEFRKLIRSGEKINAEVFGEQISELAANYQIYLNRNSL
                     "
     gene            22821..23522
                     /locus_tag="PKI35_04670"
     CDS             22821..23522
                     /locus_tag="PKI35_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNX43710.1"
                     /translation="MRLIIIAGPPTSGKTTVIKQVIRRILKKGLKPAYLKIDVMFADE
                     DEQIKKEFSIPAKKIYSGELCPDHCNVVVLDEVVEWAEKEQADVLFVETAGLCLRCSP
                     YVENCLGIVVLEATSGMNLPRKIGPMLTLADICVVTKIDLVSQAEREVFRSRILDATK
                     GTAVVESNALYGIGIDPIVRDILKTDPVEMPMFLRGNPPVGTCTICVGKKEIGSKNHF
                     GVLRTMENELFYVGE"
     gene            23924..25084
                     /locus_tag="PKI35_04675"
     CDS             23924..25084
                     /locus_tag="PKI35_04675"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNX43711.1"
                     /translation="MKSVYLDNAATTPLDEQVLQLMDEVYRKNFGNASSNHRYGTEAK
                     YSLNRYRSRLAEMIQAKPEEIIFTSGGTESNNLALKGVAFANRHRGNHIIVSAIEHDC
                     ILNTCKWLGNQGFIISYIPVGSDGIVDTGILERMINPKTILVSVMHANNEIGTIQPVE
                     EIGRICCEKNVLFHSDACQSFGKIPVNVEGMQVSLLSLNAHKIYGPKGVGCLYVRKNT
                     PITPLLHGGGQEFGIRSTTENLPGIAGFVKAAELCQEEMENEPKRLATLRDQMIDELQ
                     EEIEGFYVNGSLTHRLPQNLNFGISGLEGETMRLLLLLDEAGIAVSAGSACSNNDSSK
                     SASHVLQAIGKNQFEARGAVRVSLGRFNQEDDIRWFLDSLRQCIRHLNPIFS"
     gene            25091..26176
                     /locus_tag="PKI35_04680"
     CDS             25091..26176
                     /locus_tag="PKI35_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNX43712.1"
                     /translation="MAEITLPHRYHRHLLVPEIGYRGIRAIMSSSVIVIGAGGLGCPA
                     LQYLAAAGVGRIGIVDYDIVSESNLQRQILYTPADLGYLKADRAAARLKELNPLVDIY
                     TYPVKLNSNNALEILRDYDVILDGSDNFPARFLVNDACVILDKPFVFGALYQFKSQVS
                     VFNYQGGPTYRCLVPEYPDSSESLNCDDVGVLGTVAGMTGCIMAGEVIKIIIQEGNVL
                     SGKLMQMDCLTLQVDILTIKNNSELPKIQELLDYEELCNAKFDQWPVRSITPATLRLL
                     MNENFSGQIVDIREPEDFSRFSIPGSINRSASEIETNPDLISGHHPVIFVCKHGIKSE
                     NTIRILIRKFGFSNLLNLEGGICGWLH"
     gene            26355..26891
                     /locus_tag="PKI35_04685"
     CDS             26355..26891
                     /locus_tag="PKI35_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43713.1"
                     /translation="MAKSATGCHSCAADQVGIGEKNGWKDSLNRDYDFILDIFPDEPG
                     PKETILPYNPALVKELGVKKGDIMIGRPMGMSCGCPITHCGVVTGVDARNGVIFWCVT
                     GPLRPRTEGFIDIGYYVSQSYEGIIREWKSDIRLGTRYWFLPRRCMLQWRHSGLVNAI
                     TKLKDGSYKVRIEGLLIG"
     gene            complement(27379..28734)
                     /gene="hydA"
                     /locus_tag="PKI35_04690"
     CDS             complement(27379..28734)
                     /gene="hydA"
                     /locus_tag="PKI35_04690"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HNX43714.1"
                     /translation="MPKTDKYLIINGTVVNADESMQADVAISGGKIEAVGRMNPADFP
                     DFKVIEAEGRYLIPGGIDPHVHFALPTPAGCSSDDFVTGSKAALAGGTTSVIDFVTPE
                     RGQSLPEALELRRTEARESLCGWKLHMGISEWNKRVSEEVRRCIRYEGITSFKTYLAY
                     RDTIGISMNELEQLMRVAGPEGGTVLVHCEDGEMISRLQKQYIASGKTRAAFHALSRP
                     VAAEVNAIRKVIDLSEKTGCPVYIVHVSTGEGAGAIREAKEKGLKVFGETCIQYLILN
                     NSVYDASLPDRQVLPYVISPPLRPEPERLKLWDELSRGAFDTVSTDHCPFNLHGQKDR
                     GINDFTKIPGGAGGIEFRLSLLFTFGVLEKKISLNQFVSLTSHRAAAIFGWGDTKGKI
                     AAGFDADLVIWNPDKEWMISAKEQYQRCDSNIYEGIKVKGLAEKVFISDSRYCNPDLR
                     V"
     gene            complement(28836..30101)
                     /locus_tag="PKI35_04695"
     CDS             complement(28836..30101)
                     /locus_tag="PKI35_04695"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNX43715.1"
                     /translation="MLLLKNGTFINRQTPDFIRKDVLVEEGINGRIIIGPAPDQTAGS
                     RSIDCSGKYITKSFACGHHHAYSALARGMGAPKKEPASFNEILQYVWWTLDKALDADI
                     IRSCAMVTAIACAKNGVTFVIDHHASPFAVEGSLQIIAEAFDAAGVGLLPCYEISDRD
                     GTDIAAKGLNETAAFLSGHQGLVGLHASFTVGDDTLKRAVRLAEQTGSGIHVHVAEAR
                     SDQEHCLKNYGKRVIERFAGAGVLQFPKTILAHCLHLDDHERALVAGSPAWVVENMES
                     NLNNSVGLFDAQGLGDRIMLGTDGMHSDMLQSAKSAFLAGKNSDAPGFEETCRRFRNV
                     HRYLDENHFDGDGDNNLVVLDYDPPTPFGSDNFFGHFLFGIESRHVEHVISQGKVIVE
                     NRKIVTVDETEIMKEARCQAGRLWKRMES"
     gene            complement(30372..31385)
                     /locus_tag="PKI35_04700"
     CDS             complement(30372..31385)
                     /locus_tag="PKI35_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43716.1"
                     /translation="MNSMKPAVSINEEICRKTGLLIKELRPRKTFYERPFLKIPAPEE
                     IRLRGYFYAVAICHQTYNLHHPGLNLYGWDYIEHVFSGLMKDQHQLLQPGFLAGTGNA
                     GVQSLLAGLFSPDQKPENTSLDRLEERAFMLTELDRFVGDNFRSSLEYLVQNANGYLV
                     FRGKGFYEILSDLAAFSDPKRKKVTFLIKLLEEARLVKVKDTCNFIPIMDYHMQRVLM
                     RLGCVEVNDSSLREKLVAKILLPDDIEIRNTCIDAFRLISDISGHAVTKLNDFFWSLG
                     RSCCNYEPLCRTGRCEKQPCTFFEIVELQEHTRCRFEKVCKGFQDEGYRQLWQPIVDT
                     NYY"
     gene            complement(31382..33946)
                     /gene="xdh"
                     /locus_tag="PKI35_04705"
     CDS             complement(31382..33946)
                     /gene="xdh"
                     /locus_tag="PKI35_04705"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HNX43717.1"
                     /translation="MIAFTLNGKRIGYSGDGDRTLLDYLKLSERITSVKDGCSGQAAC
                     GACMVEINGKAKLSCTQKMKSLENACVVTLEGIPAEVRDVIANAFVGKGAIQCGFCTP
                     GFIMRTKTLFSENPKPTREEIKKAIGLNMCRCTGYIKIIDAISEALENLRTGNKPEIP
                     PLQAGIGKPFLKHEAYDTAIGKRKFINDMHFEGMLHAALRFSDHPRARVLKIDTAEAG
                     KLEGVICIFTAADVPGDRYTGLIFSDWPLMVAIGETTRYIGDVIAGVVARNETVARKA
                     AALIKISYEVLEPVSDINKALEPGSPQVHPDKPNLLEKCIVRRGNQAEEIFQNSDFTV
                     SGFYETQRVEHAFLETEAAIALPEGEGLHLFSQGQGIYVDRQQVASLLGIDEEQVHVT
                     LVPCGGGFGGREDMTVQGHVSLFAWLLKKPVKLHLAREESIIMHPKRHPVRMDISLSC
                     NRDGILTALKLRAAGDTGAYASVGTKVMERVAGHATGAYYVPSVDIETLTIYTNNIPS
                     GAMRGFGVNQVTFALESCIDELCKKGGFDRWKFRYDNALDTGKMTATGQVLEAGVGIR
                     QCLLALKDSFYNAKYAGLACGIKNSGVGNGMTDFSDVKIRVLEGGRFLIEHGWTEMGQ
                     GIHNMAIQTLYQETGIDPQLISVAVDTRAEIPTGMTTSSRATALLCNAIINAAASLRT
                     DLAHKPPEELAGNTYTGSYYCNWTTKPGAEAEKVVTHYSYGYAAQLVILNDSGEVEKV
                     IAAHDAGKIMNPMLFEGQIQGAVHMGLGYALSEDLPMEGSRLVSTKLRDCGVLPASQT
                     PEIEVKGVEVPDPVGPYGARGIGEIGLVPTAAAVANALCIFDGIRRHTLPIKRKNK"
     gene            complement(33953..35197)
                     /gene="thrC"
                     /locus_tag="PKI35_04710"
     CDS             complement(33953..35197)
                     /gene="thrC"
                     /locus_tag="PKI35_04710"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNX43718.1"
                     /translation="MKEKFHYCCCNCGAEYPGNKVIYLCPVCSGQNQPGQPKKGVLSA
                     VYNYKEIKETYRNSLFGKLEEQNFLPLLPINGCASLPKLKIGNTPLYHIEHFDGEELP
                     FDLLLKDDSQNPTFSFKDRASALVSAWAKENGIGTIITASTGNAGSSLAGICAAQGQK
                     SVIFVPATAPLAKLTQIVMYGATIVPVDGTYDDAFSLSIKVTEAFGVYNRNTAYNPLT
                     IEGKKTVAFELFSQLNYMAPDRIFVPVGDGVIISGVYKGFEDLLLLEVIDHMPVMVAV
                     QSEGSSNLIRNLGNREFRSVPSSTFADSISVNIPANFHMTAGYMGQYKGEAVIVTDDE
                     ILDASVSLAHSTGIFAEPAAAAAFAGMLKYRSEKRIPEGSKNVVLLTGSGLKDLKSVQ
                     PVIRLPEPVKPEISSLEKIFTK"
     gene            complement(35228..36700)
                     /locus_tag="PKI35_04715"
     CDS             complement(35228..36700)
                     /locus_tag="PKI35_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNX43719.1"
                     /translation="MIQITDKVINREILQKAISRYREKGIILPTFAQMRDPELVPDKV
                     KDQLKRIGLWELHPLNLFRITWKNEPKDSGGLYGSPNYIELPRSLTGIDARIIVLLGK
                     WFPTGAHKVGAAYGCLAPRIITGQFDPTFHKAVWPSTGNFCRGGAFDSYLMDVGAVAI
                     LPEEMSRERFTWLREIGAEVIATPGCESNVKEIYDKCREISCTRPDCIIFNQFDEFGN
                     SCWHYEITGHALEEVYLNLAGKNSQLAAYISATGSAGTIAAGDYLRTRFPLLKVVASE
                     ALQCPTLLMNGFGGHRIEGIGDKHIPWIHNVKNTDVVTAIDDEDCMRLLRLFNEKAGH
                     EYLTGNGVDAEFTAKLWMIGISGIANILSAIKTAKYFGMTSDDILLTIATDSSEMYRS
                     RLKELNDERGNYSVMQAAKDHEKCMIGASVGYMKELSYNDRKAIHNLKYFTWVEQQGK
                     DVDDLRQLWDDRQIWPQLFSQPARWDELIHEFNEQTRLLK"
     gene            complement(36808..40104)
                     /locus_tag="PKI35_04720"
     CDS             complement(36808..40104)
                     /locus_tag="PKI35_04720"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX43720.1"
                     /translation="MSDKFSPLPLPSLLSLTLSEIRCHRFLGLPENLFFIPGQADPFR
                     MERYGQWLETPVGVAAGPHTQLAQNIIAAWLCGARYIELKTVQTLDDLCVAKPCIDMQ
                     DEGYNCEWSQELGIRQSFSEYLNAWIIIHILRHEMGFTAPLGTIFNMSAGYNLEGIKK
                     DNVQWFLSKMQDCSPELTKAIHSIRQVYPAIGHIEIPSCISGSITLSTMHGCPPDEIE
                     KIGSYLLREKKLHTTIKLNPTLLGKEDIRNLLNDQLGFTAEVPDAAFDHDLKYPEALT
                     ILKNLQATADAGGLQFGVKLTNTLECINNKPGFDEKESMIYMSGRALHPISVKLAEKL
                     QNDFNGKLDISFSGGVDCFNIAEVLSCGLKPVTVCSDLLKPGGYGRLRQYFTNLRTAF
                     EEAKAGTIGDYIRNIAGRDDLSVDESALLNLERYAGRVADAPAYHKNTWTDFSVKTGR
                     QLDFFDCIHAPCTDTCPASQDIPEYMAHTASGSFPEAFDTIFRTNPFPSVTGMICDQP
                     CRVKCTRVNYDEPLAIRDIKRFIAESHHGEPDSGKIIPSGKKVAVIGAGPAGLSCAWY
                     LRLAGCDVDIFEQKKVPGGMVSAAIPSFRLTGEAVERDIQRILASGVQLHDNHTVGIN
                     NFDAIRKSHDAVFLAVGAQTSAKLNIEGIDSGGVLDPLKFLAEVKESIPTGIGKNIVI
                     IGGGNTAMDTARTAFRLTGRDGKVTILYRRTRKEMPADKSEIRAALEEGIEIMELANP
                     ERIISHDGRVTSIICSRNIPAMKGVDGRPVPIKVPGSEFEVPCDTVIPAIGQQPDIRF
                     LQTANLKTFPGSCKTSLEGVFIGGDAIRGASTVINAIADGRKAAAEILDSLGLTPVRV
                     ESASVRSSGFDDLVRKKATRIYGISPKEIPTDDRKNFKPVIGSLTPSEAMTEASRCLQ
                     CDELCNTCVSVCPNLANFSYSIEPVHFYLQKAVLHHDGQIRIEADEVFTVSQRRQVLN
                     IRDFCNECGNCDTFCPASGSPYRHKPGLCLSAPTLVSEEEGYLLSRLPGRDVLIYKHH
                     EHVKTLTLQDNHYIFETDQVWAVINPGDFSLKEVRFLTPCVRECRFTFAAEMSVIMQG
                     AMQLKYQITV"
     gene            complement(40354..40566)
                     /locus_tag="PKI35_04725"
     CDS             complement(40354..40566)
                     /locus_tag="PKI35_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNX43721.1"
                     /translation="METLKFKTNLKCNGCISAITPTMNNIQGIASWKVDLAAPERLLT
                     VECDPEKAADILAGIKKAGYEIELIK"
BASE COUNT        10520 a         9593 c        10135 g        10481 t
ORIGIN      
        1 ttcttttaac tttccagccc catcatggtt ttgcaggtct tcgtataaag gtcgcggaaa
       61 atgttatccg ggtcggtccg gcgctttaca acgccgtaat tctctttatt ccagattttc
      121 cagaactcct gctccgtgta atagttgttc gagatcagcg ttttgatgcc cccgatgtcc
      181 gtcagttctt cctcaatcat cctatagtag ttcttgctgc ctgtcttttt catgccgtag
      241 atggcaatat ccaggaaaag ttcgtcatcg atccggcgcc ggaaatcgtc cgacaaccat
      301 tcgtacttcc tgacaagttt gtacggcacg caccacagcg ggaaatgacg gagctctttc
      361 tgataccagt ccatgaaacg gccgactttt gaaaacggaa taaagagatc aatggtgacc
      421 ggaatcttct caggagggat cagctttcgg aatttttcga cgagtttcag caccctcgcc
      481 gagttcataa atttaccgaa gatgagcctt gcgggaaatg aagcaggcgt tacattggtt
      541 acacccctgt cgtacctgaa gaagtaatcg ggcgttttca gatagtcttc tttccgtttc
      601 cccgtgcttt cataatatac ccgcaaccag tcataacgat gggtgtaggg cgcccggtct
      661 gtgaaactgg ccgtgctgag cacatagctg gtttcggagt gtatgatccc gtccatgaaa
      721 tcaatatcct gctcctgata gtgcttccat atcgccgatt gataatcccc gggattatca
      781 aaactttcat aggtcacttt tacaaagggc agggccggta tcaaacggaa cttcagtttc
      841 gagataatgc cgagagtccc gaaggcgccg tgcaccatct ggaacaggag gctgttttcg
      901 ttatcggggg tgcaggtaag cacttcgcca gtggcggtaa tgatttcgta ttcaagacag
      961 gtgtcatgaa aaccgccgta tttgtacgac attgactcaa ttgaacatcc ggctaccgct
     1021 cctcctatgg tgatggtctt cagttcgggt acgataatgg gaacaagccc atgcttcagg
     1081 gttgctttta ccaggtcgat aaatgtaacg cccggttccg cttcacagat caaattaccg
     1141 gtatcaatag agagtatctc gttaaagtcg ctgacgtcga tcttctcatc caggcgcctt
     1201 ttatcgttaa gtttcggtac ttcatgagaa acggctttct ttctcaatga taccggtttt
     1261 gtgcattcac gttccttcag ttgcctgacg atccggtttg ttttatgctc atgatcctgc
     1321 attggtggcg gggaatttaa tgcgggtggc ggctggatta tgacaagtgt tatgatggta
     1381 atacagtctt gccgattttt acgtgatcct gactgaaatt tttgtgcccg tttccggcat
     1441 attccggctc ttgttcctga tcatttccct gctaccggaa ccttgaccgt cctgatgaca
     1501 ggttccgaaa atgtcttttt cccgtaacgg ggggtaaggc ttatttccag atagggcttg
     1561 tcttcataat acttggtttc caggatcacg gatattttca cattgacatc aataccctga
     1621 acctgattgt cgttcagcag cccgatgtaa aaagtcccct gcgtcctgtc ggtgtttttg
     1681 ccgtaagcgg caattccttt tccgctgtcg aattgcacga aatctttgtc ttccatgaac
     1741 agccgggcat tggcatagtc agggataaac cagtaggcta catcttcacc catcgaaggc
     1801 atggcaaggg ttgcaagggt gccaatggcg attccggcaa gcgggctgcc gccgaggatg
     1861 acggatgcgc cggaagcgat atttttaata atattcacat ttttcagcca tgcttcagag
     1921 gcttctttgc cgacgccgat ccaataggcc cacgacctga ccgtttttgt cataaagggc
     1981 gtgacctcat tttgaggcag gcttaccgga agaatactgt aatttttgtg ttgcaaattg
     2041 ttcctcgaat gtaccctttc cgttttgtct gcgatgatat cttccgagat ttcggtttta
     2101 accaattctt ttttcgtctt ttgaatgtag gatgtatcgt agccgattac agcttcccgg
     2161 gtacaagtgc tataggttgt atccggacgc atctcccagg taacggcggt gttgaaattc
     2221 cgggtatcgc cgctgacggg aacccgctga atcttgatga ggcatacccg gccggccaac
     2281 gcggaattct taatacggaa aatataaaca ccctgcgtgc ttaccgtaat cgttttattg
     2341 tccacatggc tggttttgta agctgagaat ctcgagttgc cgggatattc agtaatttct
     2401 acctctttga tttcctggcc gcctgtttcc ctgaaactga agatgatttt atcaccggct
     2461 gcaaagccgt agtatagttc ttcttcctgc atgcccccca gtttgatggt ctgttcggtt
     2521 acgtcgatcg gctcctgccc gaaagccgtg attgctgaaa atagcaaccc tgcaaatatt
     2581 accggaagtt tattcataat cattatgttg atctctgccc atcccacgct ttcttttcat
     2641 tttttgcggg agcaaaggaa attcacccga atcatcagaa atactcgctg acgaagggta
     2701 caaatattat aaagaaatga tattatttca aaacatgttt ccagtgtcaa ttttgataat
     2761 tggctgttat attgccggtt aggcgtatcg gaattatttt cgcactaaca tgttaccggg
     2821 agatgaaaca gcggagaaaa caaatgtttt gtcagggacg ccggcaaccg gagaatgttc
     2881 cggcaaccag ttttcaaaac cgggaattta tcattcagga aggtacaacc tgttttgcgc
     2941 ccatcttcgg atctcttttt cccggtgttc cgtatgattc cggctcgatt tttcagggaa
     3001 atatatatca tcctgttata tgtcggccag catgggcagg gaggatgaaa gaatgcctat
     3061 ttttgcaaag caaaactgta cgtggaaata atggataaat cattaaaaaa cagtattata
     3121 gcgcggtcgc acgaacgatc gagaaagttt ggcgttgagc ctgaccgtat ctttccacgg
     3181 catattatca ggggagccga tttaaagcgg cagtttgcgg ttaatgaaga actgattaac
     3241 gtttcggatc cgattatcaa tcaattgttg tcttcggtga atgaaacagg gtttatcata
     3301 gttctgaccg atgctgtggg atgtattctc atcatcaaag gaggggaaag gacgcttgaa
     3361 gctgccgcga aactaaacat gattcctggc gcagtgatga gcgaggagag tatcggcacc
     3421 aattcgatgg ggactgccat cagtgaaaac gcccctgttc agattacggc aaaagaacac
     3481 tttatatccg tttatcatca atggacctgt tcggcggctc cgattcattt cgaaggaaaa
     3541 ataatcggca cgctcaacat taccggatcg gccgatcagg ttcatccgca taccctgggc
     3601 ctggttcagg ctgccgttga tgccattgaa aataaattga ttacaaacaa ggtgcaatcg
     3661 gaactggagg catccaatca atatgctttc gccatgatga ataaccttgc ttacggagtt
     3721 ttcgccatcg atcttaatga tgagattctc tgggtaaacg acagcgcctg ccgtattatc
     3781 aatatccgcc ggacaaagtt gctgtacaag ccaattaaag aatttgtgcc agcctgggaa
     3841 aagattgccg atacattgct caatgataaa ccctttattg accaggagtg taaactggcc
     3901 gtgaaggacc tgaaggaaag gtttcttttt aattcctacc tgataaaaag cgaaaacaaa
     3961 gaaatactgg gctacctgct aactttccgg caatacagca atatgctgaa tctggtaaac
     4021 agatacaccg ggttccaggc tggttacacg tttgatgata ttattgccca aagcaggtcg
     4081 atgagtgaac tggtcaacta tgccaggacg gttgccaaca gtccctcgac gatactgata
     4141 acgggagaga gcggcaccgg gaaagaggtg tttgcccagg cccttcataa tgcaggtgag
     4201 cgcagcgagg ctgcctttgt tgccgtaaac tgtggcgcca tttctcccac cctgatcgag
     4261 agtgagctct ttggttatga ggaaggcgct tttacaggcg ccaggaaggg cggacggccc
     4321 ggtaaatttg aacttgccag cggaggaacc attttcctcg atgaaatcgg tgaaatgcct
     4381 gcggatatgc aggtaaaact gctgagggta ttgcaggagg gcgaggtaac ccggttggga
     4441 ggcgatagca gcactaaaat agatgttcgc attattgctg caaccaataa aaacctggaa
     4501 gccgaggttg aagcgcgccg tttcaggctt gaccttttct accgcttgaa cgttattccc
     4561 attcatattc ccgccctccg cgaccggcgg gaggatatta tccccctgtg gcagcacttt
     4621 atcgcaacaa aagctgccaa gcttaataaa cctgttcctg agctgacgaa ggatatcatg
     4681 gataaactga tgtcctattc ctggcccggc aatatccggg aactcgaaaa ctttgtggaa
     4741 aaacttgtca acttcggagg cgtacccgaa cttgccgata cgccggccga aacagaccaa
     4801 aacggggcag aagtcagtgc ggccgtcaaa aaaatcaata gccttgaaga gaccgaaaaa
     4861 gaggctattt gtgatgccat caatgcgctt ggcggaaata tttccaaggt cgcaaaacaa
     4921 cttggcgtag gcagaaatac gctctatgtc aagatgaaga aatacgatat aaaactccgg
     4981 gagtaacagc ggatcccgga ttcccggata ataaaacccg gacaggcgag gtgttgaaaa
     5041 cattctgcat gccggctgat tcctcttctt aatctttcct catttccttc cctgctcctg
     5101 aatttcattt caggttaacg acattttccc ggctaccggt aagtacttcc atgcctgtgt
     5161 tcacataccg aacattgttc gggatttgaa cacacgacaa ccggataagt gttccggttc
     5221 atgacacttt acctggacaa ataaatcaat cagctatata aacggcagat aatcaaattg
     5281 ttaaaaatcg tggcaagcta gttggtaaag gagggccaac ttactctttt gttaaatctt
     5341 aatgcacaat taaaatgaat cgtttcacat taccgagaga cctgtattac ggaaaaggtt
     5401 cgatggaagt tctgaaaacc ttaaaaggga aaaaagctac ggttgtcgta ggcggaggtt
     5461 ccatgaagag attcggattc ctcgacaagg ttgaaaacta tttaaaagaa gccggaatga
     5521 aaattcaggt tatcgacggg gttgagcccg atccgtcggt tgaaaccgtg atgaaaggcg
     5581 caaaactgat gcagcagttc cagcctgatt ggattgtatc aataggcgga ggttctccta
     5641 ttgatgcagc aaaagccatg tgggtgtttt atgagcatcc cgaatcaaag ttcgaagata
     5701 ttgccaagcc tttcaatatt cctccgttac gtaacaaggc ccgttttgtc gcaattccgt
     5761 ccaccagcgg tacggcaacg gaagttacgg ccttttcagt cattactgat tacagcgtgg
     5821 gcatcaagta cccccttgct gattttgaaa ttaccccgga tatcgccatc ctcgatcctg
     5881 aaatcgcata cactatgccg gcaaaattaa cggcatacac gggcattgat gcactgacgc
     5941 atgcaaccga ggcaatcacg gccagccagg cctccgattt ttccgatccc cttgcactga
     6001 aggccatcgt catgattaag gacaacctgg aagaatctta tgccggcaat gtaaccgccc
     6061 gcgataacat gcacgttgcc caatgtcttg ccggtatggc tttctcaaac gcgctgctgg
     6121 gaatttgcca cagcatggcc cataagaccg gagcgatatt cgatatcccg cacggttgtg
     6181 caaatggcat ttacctgcct tatgtaatca ggtttaaccg taagaatgcg gaagccaggt
     6241 atgccatgat cgccaggttt atcggattaa aaggtgaaaa cgacaaagaa ctttgcgatg
     6301 cgtacatcaa ctacatcgaa gcgctcaatc aaaagttagg gatacctgca acccttaagg
     6361 agtatggcgt aggcgaaaac cttttcaggg aaaaactcga taaaatatcg gagaatgccg
     6421 cgctagatgc ctgtactttc agcaatccgc ggcctgcaac accggatgat ttcaggaaaa
     6481 tcctcacggc cgctttctac ggtacaaaag aaaacttctg atgcagatac aataatacgg
     6541 ggtgggcgca gaatcgccca ccccgtattt tacaggcaac catgataatg tttgaaataa
     6601 ctgaaaatac ttatcaggta ccggacatcc gcccggtgca agttaaggac cctgccagga
     6661 taaaaaccga taatccggtg ctgtcttcca ttatcatagt tgtcgcaatc ctgaaatcag
     6721 tcataggagt aggtatgccg acagcgcctg ccaataatgg tttcacttta taaatgaatg
     6781 agcgccgccg gaaaagagtc tgtccattat acatgaacca caagctgttt aatagaacag
     6841 aatgccgcta aactgaataa gccatgaaca acagaatgaa aattgtgatt tgccttggca
     6901 gctcatgcta caacagagga aatcagtatg tgcttgaggc tatcaagaaa tacttaaacg
     6961 aacataacct gaaggacaag gttgatttca ggggacagct ttgctcggga aactgcgcac
     7021 aggggccggt tctgcatatc aacgggaaga cgtacaagga gattgacgaa aacgtcgcta
     7081 tccggttact tgacaatctg tttgctgcca ataaaaatta accgggatgc ttcaggccat
     7141 ctataccgaa agaaacaact gccaggattg ctacaagtgt atacggcaat gcccggtgaa
     7201 agccataaag attgaggatc acagtgcatc ggtcatcagc agtctttgtt tgtactgtgg
     7261 ccggtgcgtt caggtgtgtc ccgtaaacgc caagaaagtc cggaacgatc tgaatgcggc
     7321 caaattccat ctttcagcaa gcagccgtgt cgtactgtcg ctggcgcctt catacaagag
     7381 cgagttcacc gattatacat taccgcagtt gctcgaaggg ctatacggtc tgggcttttt
     7441 taaggtttcc gaaacggccc tgggcgcaga actcgtgtcg aaagccacaa agaaatggct
     7501 cgatgagcaa cccgatggca tttattattc ttcctgttgc ccttcgattg tccggctgct
     7561 tcagaaatac tatcccgaac aactgagaag acttgccccg gttgattctc cgatgcaggc
     7621 ccatgccagg ttcatcagga aaatgtatgg cgaacacacc tgtgtgatct ttgccggacc
     7681 atgcattgcc aagaaaaagg agatcgaaga ttatcaggga ggggccgatc tggccatcac
     7741 ttttgcggaa ttgcgtgaat ggcttaaggg tgaaggcctt acgccggatt tcctctcagg
     7801 ttccggaggg tattccttcg ccccttataa tgctaacaat ggaagtattt acccggttga
     7861 cggcggaatg atttccacca tgaagcaaaa tgtgggaata gctgatattt cattcatgag
     7921 cttctccggc cttcagaatg taaaaggaat tattgagcag cttcctgaaa agtacgaagg
     7981 gaaactcttt atcgaactca tggtttgcga agggggctgc atcaacggcc cggttgtttc
     8041 acacccggga tccacggtcg ggaaaagggt gctaaccctg aaacaaccgc tgaataatgg
     8101 tgtcggtatc agggatgttg aagataaggg aatggacctg tttgccgact attccggcac
     8161 gaagccggtt acacgttgtg tccacaccga aaaggagatt caggatgcgc tgaaaaccgt
     8221 cgggaaggtt accgataagg atgaacttaa ttgcggcggt tgcggttacg agagttgccg
     8281 caggtttgcc gaggcaatgc tggacgggaa ggcagagcgg aatatgtgcg tttcgtacat
     8341 gcgaaaggtg gcccagaata aggcaaccgt tcttttacag aaaatgcctt acggggttgt
     8401 gctggttgac gataagctga aaatcattga atcgaaccag tattttgcgc ggatgcttgg
     8461 agaggaagcc atgaatattt attcggtaaa accgggaatg gaaggcgccg atctcaggaa
     8521 actgacaccc cagtataagc tatttgccaa tttcctcgaa aaagggctcg acgaattcaa
     8581 caaggatatt cgaatggatg atggccttta ccatctttct attttctcca tccagcagtt
     8641 caggattgtt tgcgggataa tccacaagct ggatcagcag cactttagca agaatgccct
     8701 tacggagcgg ttgcataaag ttatcagcga gaatctggcg acagcccaaa aagcagcgtt
     8761 catcctgggg gaaaatgcat cgaggactga aacgatgctt cacaatatca tcgactcata
     8821 tcaatcggac gaggatggca acggctgatt atttcatcga gattgcccac aggaatattc
     8881 agaaagcagg gcaggaagtt tgcggcgatg ttttcttaag caggaaactc tacgatgaag
     8941 gccggacaat atccgttctt tccgatggac tgggaagcgg tatcaaggca aacatcctgg
     9001 ctacactcac cgcatccatg gcgcttaatt ttactgtgga gcatcagccg ccggaaagaa
     9061 cttcccaggc tatcagggat gcgcttccca ttgatcagga ccggaaaatc agttatgcca
     9121 ccttctcgat cgtcgatata gaatacgata atgaaaccag catagttgaa tacgggaacc
     9181 cgcctttcat tgtattcagg ggcgcggctc cttacaaagg catcatcagg aaggatttta
     9241 acgcagatat taaaaaatgc aatttttgcg cgcagcgcga ggatcggatt gttctctttt
     9301 ctgacggcgt ttcacaatcg ggaatcggac ggattgaaat gcctttcggc tgggattccg
     9361 aaaatattga aaactatatt tcgggtatcc ttgaaaaaaa tcccggaata tcggctgaag
     9421 aactggcaca aaagattatt ggcagggctt acatgaacga ttcgatgcat gcccaggatg
     9481 atatcagttg tattgtgatc tatttccgga agccccgcaa gctgttgatt tgtagcggcc
     9541 ctccctataa gaaggaaaag gacagattat ttgcggatac tattgagtgt tttgacggca
     9601 ataagattat atgcggggga acgacggctc agattattgg ccgggaactc ggtcttgaaa
     9661 ttgagacaga cctcttttca ggtgataacc aactgcctcc tgtgtcgtat atgaaaggga
     9721 tcgatttggt caccgaagga attcttacca tcgggaaggt tgccgaatgt cttgaaagcg
     9781 gtgaaatgct gccggaaaaa aatccggcag gaaaaataat caggtatatc ctggccaatg
     9841 atcatatcag gttccttgtc gggacaaaaa tcaatgaagc gcatcaggat cctaatctcc
     9901 ctgtcgaact tgaaattcgc cggaacgtaa tcaagaaaat agctcagatc cttaaagaga
     9961 agtacctgaa agaggttgaa atccagttta tataacacga aaacgaaaaa tgatcatgag
    10021 tgctgaaaag aaagtagttg taaaaatttg taccggtacg ctgtgccatg tgatgggagg
    10081 ctcagagctt ccggaactcg ccggtcatct tcccgaaagt ataaaaccaa aggtggaaat
    10141 caaagggatg acatgtggcg gattttgcaa ggatggcaac agaaaaccgc cttttgtgac
    10201 gattaatgat gtcctgatgg aacaggcatc tatcgaaaaa attgtaagca acctggaaaa
    10261 agcaataagt gatgacgccg aataatcttg ctctgttaac aaggcgggaa ctactggttc
    10321 atttgtgccg gatgcttgat cagggaacga tactggatga aatcgacagg ctgccggtcc
    10381 tgatgcgtcc caggcataat cagccaatcc ggtgttgcgt ccacaaggac agggcaatac
    10441 tgaaatataa gctgatggcc ctgctcgctt tcaatgttga cgatgaaacg gatgaactct
    10501 gtcagctcca cgaatatgcc gccacggccc tgaaacgaga aaaattaacc cctgtagcca
    10561 tgacggtcgt tgatgaggca tgcagcagtt gtaaccaggg gagttatgag attacaaacc
    10621 tttgccgtgg atgtgatgcg cgtccctgcg aggtgaattg ccccaaaaag gcggtttctt
    10681 ttgccgatgg taaagcccgt ataaatcacg atagctgcat caactgcgga aaatgcatga
    10741 aggaatgccc ctttcatgct atcgtttatc agcctgttcc ctgcgaagag tcgtgtcccg
    10801 tcggcgccat tgaaaaggat gacacgggga tagagcgaat aaatttcgat aagtgtatct
    10861 tttgcggtaa gtgcatcgcc gcctgcccgt atggggccat cgttgagaaa acgcatctcg
    10921 tggatgtttt cagggcctgg aagagcggac gcaaggtcgt ggctcttgtc gctccggcca
    10981 ttgccggcca gttcaggcag gatctcggca aaatatactc cagcattctc agcgccggat
    11041 ttcatgatat cgtggaagtg gccgaaggtg ccgatttaac cattgccaat gaatcggctg
    11101 agtggaaaga acggatggct gcaggctgtc agtttatgac tacttcctgc tgcccggcat
    11161 ttaaggaact ggtaaagaag catatccctg aaatgagtga taaggtttca tccacccctt
    11221 ctccgctatg ctatgccgca gaaatagcca ggaaaaggtt ccctgatgcc gttacggttt
    11281 ttatcgggcc ctgcatcgcc aaacgggttg aatcctatta tctcggttcg gtagatttca
    11341 tgatgtcgtt tgaggaactg ggggcattgc tggttgccaa aggaattgag gtcgccgacc
    11401 tcgaatcgca tccccgcccg gaaagatctt cggcgcaggc ttatcatttc gcttattcag
    11461 gcggcgttgc cggttccatc gtggcgagtg ccggttcatc ggtgaatgca gaagtgatca
    11521 atggcatcga taaaaaatcc atcaccctgc tgaagcaaat ggcaaagggc aaaaccgata
    11581 aaaattttgt tgaagtgatg tgctgtgaag gcggctgcct gggaggcgtc aatacgctga
    11641 taagaagtcc ccaggcggtg gaaatcttca aaaagaatct ggaggcctag aattgtgtca
    11701 tcggcctgat ggcccgcctg ggcatgtagg ttgaccttgc attgcccgaa gggtatgggc
    11761 tgatcccgga agttatggat ctggctaaaa accaggctgc cgaaagcggc ggaagtttca
    11821 gggcgctgaa ttcgatggac gaagcattcc gggatgccga tatcatttat ccgaaaagct
    11881 gggctcctta ccacgtgatg gggtgccgca cggaattgtt gcggacaggc gacctgaagg
    11941 ggctgaaaga acttgaaaag gaatgccttt ccaacaacgc ccgcttcagg gaatgggaat
    12001 gcaccgggaa gaaactgaaa ctcaccaggg aggggaaagc cctttacctg cattgcctgc
    12061 ctgccgatat ttccgggata agttgcaggg aaggggagtt gtcggccgct gttttcgatc
    12121 tttacaaaac ccctttatac aagcaggccg gtttcaagcc ttacgtcgtt gccgccatga
    12181 tgctggccaa ccggtttgaa gatcccggag aaatattgaa acaattgctg gagaacaata
    12241 ggaagtggat gctttgatcc ggccttttct cccgttcaag agtacaacac cccatgaaca
    12301 ctcgtttgca ggcatggcga gcgcctgccc ggtgacggct tccgtaaaaa tcagtttccc
    12361 ctgaaaagca atgctattaa aaccgcgacc tgaaggtgtt gatttcccat ttgttcttgc
    12421 cttcgttcag ggtggcacaa agaattttat tcccgtcggt cctgatgttc acgaggccgt
    12481 aaacaatact gtccacaacc ttcagctttt tcatgacttt tgtcttgtcc tttgcattca
    12541 acgctcctga agatgtttgt ttgtaggata cccgggttgc ctcttcgtcg gtaagattaa
    12601 tatccagatt gccgttcggg gtttcaaaat tctcataact tacctggtaa ggatctccga
    12661 gtttgtaact tctcgatatc tcggtgatgt atatgagagg cgttactcgg attctatgat
    12721 gccataccag aagagttcaa accataatat actgaatccc aaaaggttta cgaagtaaag
    12781 ctgattatat tcagtaattc tgggcaagtt ccgaaaaacc aatttcgtaa acctgttcag
    12841 ccgcagtata aaactaaaaa aggctgtcct gcaaaaacag aacagccttt tttactcctc
    12901 acttccacag gctacctgat tacctgtatt ttgcgggttg cggtaccggc ttcagttatg
    12961 atctgaagca tgtacatccc tggaattaga ttttccacat tcacagtgtg ttccaggccg
    13021 gcggcttttt cccggaggat atcccttcct gaaagatctg taatccggac ttcggtcata
    13081 ggctcgacag actgcacaaa caatcggctg ctcaccgggt tggggtaagc cgatactacc
    13141 ggcgtcgcct gcaacggaga caccatggtc gttcccgttg agtaaaacag ggtaatattg
    13201 gggtaataat ctgatgaata gccgaactcg ggcaggctca tggggtcgaa aggcagatca
    13261 tccctttcgg aaatgcgtga acggcttgcc cccgtgtcga tggaactgat aaaaacagcg
    13321 cccagtacca tttcgctata acttttaacc gtttgcacca caagatttcc gccatggtaa
    13381 gtataagggt tgtccagcgg gatatagata gtgttgaaac ccttggcgaa attaatggtc
    13441 ccgttgaaaa ccggggtcag ggttgcggga tctgcccagt tatcggccag gctggacaga
    13501 ctggtttcac ccataaggat ctgtacaggc acatcttcca ggtcgttgtc gaactggcag
    13561 gtatactgca gtccggtgat cggatacccg ctgacaccca cttcttcagg catatacagc
    13621 gactgggtga agctgtgaag gttgaaaaag ttgcagggca gagagttcag cgggcaggaa
    13681 ccatttccga tctgggtact ctcgatgccg gccggctgca cgtatacagg gaggtcgaca
    13741 gtgcggttat tatcctgatt ttcatcctgg gcaaactcaa tctttgcata aatgtccatg
    13801 ctgccgatgt tatcggtgga aggagtccac cagagcagat ggtacatttt ttcaccaaag
    13861 gcaataggct ggcctgcttc tgatgacagt tgggcgccgt cagcactgta aagcttcacg
    13921 gtgtaatcct gctgtgcaag cttgcctcca tttataactt ctaagaagta tagctgttgc
    13981 tctcctgctt ccggggtggt atttccggaa aaattgcctg taagcaggtc gttctccacc
    14041 ggcgattcta caaaaaggtc atcgatagcc cagaaattta ttgcgtaatt gttgccatcg
    14101 aagcggaaag cgatttgcag cttctctgca ttctcaggag cggtaaaata ccaggaaaac
    14161 tcgtcctgtg ggatattgag catccccacg ggtttttcca tcagcacttc ccaggtagcg
    14221 cctccatcgt atgtgacatc cagtccgatg atttccccgt aatccatttc gtagttgaag
    14281 agatattgtt taaacctgag gcataactca tgataaccat caatattaat ttcaggagaa
    14341 atcaagcggc tgaggccgga agcaaaacta taacccagga acagctcggg cgcttcgccg
    14401 ccggccatgg ctgttgcgga aaccgaccag ggagcctgtg cgccattgag cgtccagccc
    14461 gggggcagtt gccctgccgg attgtcaaaa tgattactgt agagcacttc ctgccccacg
    14521 gcaactctgt tgaaaaagaa catgcctgtt atcagcaggg cagtaaaaga agtaaacacg
    14581 attttcataa aggtgcaatt aagggataaa ttctgtggca aagtaaattg aaacatcatc
    14641 aaaactcaat tggtattaca tgctatttta tcattaatct tctatcggtg ccagcactta
    14701 tttatcttcc gtggcaatgc ctattttatt cattatcaga aatcacatgg cttagctgag
    14761 cgggaaagcc ttagctttgc aatactgttc agatcacaat attcagatgg gtattattgc
    14821 aaaatcagga acgccgactc atcctgccac aagaccagtt ttgtacattt atctgatgtc
    14881 cattgtgttg cttttgtcct gctgcaatgg aaatgataaa attgtaaccg gtccgtcaga
    14941 tccggttgcg ggcgacagca tgattgaaga aatccatcag attatttacg agaatccatc
    15001 gcaggcccgg accctggcgc aacaactcct caaggatcgt tataaagata atccggcagc
    15061 aaaactaagg ttgttgaagt acataggttc gtcctacaca cttgaaacaa ggtacccgga
    15121 agctatcaac tactacaacc aggcgctgac tattgccaaa gagatcaaac tttaccttga
    15181 gattgcccat atcaacaaca acctgggcat gatctacaat gagaccggca actataaaac
    15241 cgcctacctt tatttgtcgg aggcgctgaa caattacaac ctggccggag tccctgaaaa
    15301 gatggcgggg acatacaaca atatcggatt gatatatctg aacctgaaaa attataacaa
    15361 agcgctggct tacttcaata aagcacttga ctcaacagga aagcaaaagg actctattct
    15421 gattatatcg gtgctgaaca acatcgcact ctgccatatc accggtacca atccatccct
    15481 tgcccttgat tacctcagca gggctattga gctttcacag agaatcaaca acaaatacgg
    15541 tttgtgcatt tcatatcagt tgcagggcaa ttattacctg gcacacgaac aacctgaacg
    15601 ggctttggaa gcttttaagc tgagcaccga catcgcccgc gaaaccaaac tgtcctacca
    15661 actggcagtg gcaaggctgg gcacagccgg agcgctgaac agactgaacc aaacagcaaa
    15721 ggcgcttacc atcgctgccg aagtattaca gatggcgggc gatcaaaaca gtctggtgct
    15781 gaaaagtgac gcccatgaag tgttgtcaga catttatgaa aaaacaggtg actacaaaag
    15841 cagtctgttt cactaccgcg aacacataaa ggcccagcag gaagtgatca atcaggcggt
    15901 ggtgcaccag gtttacgatg tggagcttga ttatctcagc cagaggaata aaatgcagct
    15961 attggagctg gaaaagaaag aactggctat cagcaagaag aacaacctgc tgttttttat
    16021 ttctctgaca ttcatcctgt tgctgatcgg gctctacctg ctctacctca atcaccggca
    16081 ccggcaaaat gtaaaacttc agaagacggt gatcgaactt tcggagaaaa aagccaatgc
    16141 cgtgctggaa gcagaaatcc aggagcgcaa acgcatcggc agcgagttgc acgacagcct
    16201 gggacatctg cttagtctgg ccggactgaa tgccagtgtg cttcacaacc ggaaggaact
    16261 aactgaagaa aaaagaaagt tattgcttga atcgctgatg aagagcattg acgatgcttt
    16321 taacgaagtc aggacaattt cgcacaacct ggcgccctcc ctgctttcgg aacgcggctt
    16381 gaaaggcgca ttgaaaaaca tttccgacag ggtaaatcaa agcacacgcc tgagtatgag
    16441 tttcgacacg ttcggactaa gcgacaaact ggacaacctg atcgaaaaca cactcttccg
    16501 aaccatacag gaaattgtaa acaacaccat caagcatgcc gaggccggta gtttatttat
    16561 acagattact cagggcgacc atgaaatctc gctgattgca gaggacgacg ggaaaggatt
    16621 caattttgag gagatcaaac aacactccag ctacggactt gcgcatatga aatcgaggat
    16681 agagaacctg catggaacca tgtttattga ttctaatccc ggcaggggaa ccatcatcag
    16741 cattttaatt ccattacaat aaacagctta tgtcagaaaa tcccatcaga gtcctgattg
    16801 ttgacgatca tcagttgatg atagaaggac tgaaatcgtt actcgaaggc gaagaagaca
    16861 tcgcttttgt ggccggagcc aattccatgg cagaaaccct ggcctttctt gagaaaaacg
    16921 tcatcgatgt tattctgatg gatatcaata tgccggatgg ctcgggcatt gaggccaccg
    16981 aaaaagtcag ggctttgtac ccatctgtga agatcattgc actcacgatg catgacgata
    17041 tttccatcat ttcaaaaatg atcaaggccg gggcatcggg atatgttctg aaatgcacca
    17101 atatgaacga agtaaccgag gccctgcgga ttgtgcataa gaacggcaaa tatctgagca
    17161 tcaacaccca gaatattatt atggaaaacc ttgggagcac tccggaacaa acagttaata
    17221 aaggtgatac ccgtccaacg ctttcctccc gcgaaatcga ggtgctgaac ctggtagcca
    17281 gggaatacag caatgaacag attgcggaaa agctgtttat cagcgaacgg acggtggagg
    17341 cgcaccgccg gaacatcttt attaaaacaa aaaccaaatc cattgtcggg cttatgaaat
    17401 acgccatccg caatggattg attgaaccgg aaaacgaaat cagatagcct cctgaagccg
    17461 gatcaacctg aagcctgacg gttcggaggg agatgttggt tcgcagcaac tttcatttag
    17521 atacagggtt gccggtggaa taagatgtca gttttcgtta atcaaatagg atcatcctga
    17581 ttcactgtca ttgataaata tttatctttg caaaccgacc gtcggtatgt taaaaatcaa
    17641 ctaacattat ggccgaaaat ctgccaaaag aagaacgggt gcagcaaatt gtggaggctg
    17701 ccgtagccga gttccttgaa aaaggatacg ccggagcttc tatggaagcg atcgccacaa
    17761 gggcgggaat tagcaaaggc gggctgtacc accatttccg caacaaggac gaggtgcttg
    17821 tgtttgccaa ccaggttttc atggagccgg tagaggcgct gatgcaggaa ctggttcaga
    17881 gtacgtcggt ggcgaaaggc ctggaacttt tcatccttca ctacatcaat cactggatca
    17941 cgcaccgtaa agagctggag ttttacttcc tggtaatgga caaggcattc cgcaatccca
    18001 acctgctggc ggccttcggc gattatgcgg cgcagatgct gacattcatg gaatctgttt
    18061 accaggcggg aataaactcc ggcgagttcg ggaatatcga tccgcataaa atggccatca
    18121 ccttcctgtc ggctatcgac ggatcgctgg cctacatcat gctcaacgaa gcgtttgaac
    18181 cggtgtcggt gatcgaggcc ttgcaggaaa cgctggtgat caagcataaa agtaattgat
    18241 taacagggat gtatatttcg cttgtaatcc ttcaatcaat atttagcatt cctatggttt
    18301 tgaatctcag atttctattg acaaatgaag aataccgata aaacaaaata ccctgaaaag
    18361 aaacatgatc caacgatatg ataatctcct gatgatttac tattccggga cgggaaactc
    18421 gaaacgggta tcggaatgga ttgccgaaac ggcggctgaa aagggtatga aaacctgtgt
    18481 ggcatccttc agggactttg acaaagagat gatctccgga ttcaccggca ggaccttgat
    18541 cggtttcttc tcggccacgc acggtttcaa catgccacac tccatgctga agttcctgtt
    18601 ccgtttccgt gaactgagag gttccgatat attcatcggc aacacccggg ccggcatgaa
    18661 actctcgaag tttttcatgc ccggattgag cggccttgcc ctttatttcc cggcgctcgt
    18721 catgcttctg aaagggtatc agatcaggtc gatgtacccc gtggacctgc cctccaactg
    18781 gatcagcctg catcccggac tgagaccgaa agtgatcgat tccatgtttg tccattaccg
    18841 ggagctgacg aggcgttacg ccgaaaaggt gctttcgggc aggcgcgtct tcctgaaagc
    18901 cttcatcctg ttgccgctcg atattctcgt agcgccgctg gcagtcggct attacatcgt
    18961 ggggcggttt atcattgcca aaacctttat caccaacgaa aagtgcgata actgcggatt
    19021 gtgtatgaag cagtgcacta ccggctccat tgtcctttcc aacggacggc cgttctggaa
    19081 gttaagttgc gaaagctgca tgaagtgtat gaactactgc ccgaaacggg ccatcgaaac
    19141 ggcgcattcc ttcttcttta ttctcttgtt cgcactgata ctgtttataa atcctttcct
    19201 ttcggaaaaa gtggttgcgt ttgtgaaccg ccatctgagc ggctcaggcg ttgcttacga
    19261 agtactggat ttcatcgtgg aatggggtgt agccatcatg ctctacgtaa tgggttaccg
    19321 gctgatgcac tggctgtcgc aatacaggtt tttcaaccgg cttattacag ttacgtccct
    19381 gactcactgg agattctggc gaagataccg ggcgccggga aaaatcctga agcctggtct
    19441 tgtgaaatga agtctttata ttcttaacaa atcgggcgtg ttcatctcat tgtagccgtc
    19501 tgatattatt ttccttgttc aaccagcgct tcttcagaag acaacatttg gtgtagtatg
    19561 tttgtttatc ttccattact ttcgcaatct caataaatga aatttgaatt tttcgggcat
    19621 gcccataacc agggaattga aatctatgaa ccaaactgaa gatctttttt catcggagcc
    19681 cgtatcgaag caaaaactga tcagcctgtt gtctgtcact ggtttgcgac aagctgagtt
    19741 gtttgaacat gcagcgcgag tcaggagtaa atcggtggga gatatcgtct atttcagagg
    19801 actgattgaa ttttcaaata aatgccataa gaactgctac tattgtggga tcagggccgg
    19861 gaatacccgg gtcgtgcggt atgatctcac tgatcaggaa atactggatg ccgcgaaatt
    19921 tgcattggat aacaagtacg gatccattgt gcttcaatcc ggcgagaggg aagacaaccg
    19981 ttttacccgg cggattgacc ggctggtaca gcagattgtt cgcgatacca gcggacggct
    20041 tagaataacg ctttcctgtg gagaacagaa acctgacgtt taccgtaggt ggttcgagag
    20101 cggcgcaaaa aggtacctga tgcgaataga gacatccaat ccggttctct attcgaaatt
    20161 gcatcccgat gacggtctcc attcctataa acgtcgcatg gagtgcctgc aggcgataaa
    20221 ggacattggc taccagtcgg gcacaggtgt gatgatcggt ttgccgtatc aaaccctgga
    20281 tgatctggcc ggcgacttac ttttcatgaa ggaatatgat gtcgatatgg taggaatggg
    20341 tccctatatc gagcatgctg aaactcccct gtatcatcta aaggattcgc tattacccct
    20401 ccgggaacgt ttcgatctga ctctgaaaat gatatctgtc ttgcgtttac tcatgaataa
    20461 tgtgaacata gccgcgacaa cagctttgca atccattgac aaaatgggac gtgagaaggc
    20521 gttaaaagcc ggggcaaatg tactgatgcc caacatcaca cccggaaaat tcagggattc
    20581 ctataagctc tacgataata aaccctgcct ggatgagaat cccgaggatt gccaatcatg
    20641 cctggaatgg cgtgttgccc ttacaggaaa taccatagcc tatgatcaat ggggtgattc
    20701 acggcatttc ctggacagga acccgaagga agaggctcct tcagcttaaa tacgggcaag
    20761 catcgacagg ctggtatctt tcaaacggtt aaaaatatag cttttgatat cgctgaaagt
    20821 atggcgtatt tcacaggttt cttcatcttt gcaatgttcg caaggcctgt agtatttttc
    20881 ggatgtacag gccaaaaacg caatacttcc ttcaaaaagc ctgattattt ccagcaggct
    20941 gatctcattg ggatttttaa taagatagta tcctccgttt ttacccagtt tgcttcccag
    21001 atagccgttc ttcttaaggt caagcaggat tgattcaaga aatcgcttgg gaatgagttc
    21061 actttttgct atttcattaa tcatcagggg ccctttgccg tattctcttg ccaatcgtgc
    21121 aagagctagc atggaatagc gagttttttg cgtcagcata tctacccaat tttacttatt
    21181 agatttagcc tttccatatc cagaatttcc aattgacggc ctgtgatttg aataacaccc
    21241 tcattctgcc atttcgacaa ggtcatgatc aggttttcgg ccgaacatcc ggcaaactcg
    21301 gcaagctcct tccgggtaaa ggaaagattg aattcgttcc tgtgatatac ttcgttgacc
    21361 aaaaacagga tgatatcggc aatccggcct tccttttgct tggatgcaag gctgataaaa
    21421 ttcatgatgg aactcttgaa catctggcat gcaatctgaa acaaagcaat ggaaaatctt
    21481 gcattcttcg acattacact aagcaggaca ttggaattta tgagcactgc ttcgcattcc
    21541 tccaccgcta tgatcgaaaa caggttattg tccttgtaaa agaggtttgc cccacctagg
    21601 atcctgggag ctgaaaccac ggtcaggatg atgttctttc ctgattcatc ctcaaaattg
    21661 aatttaacga gtcctttctc caggaagacg atgtgagaag gaatattacc ctgcttgagg
    21721 atagtctccc cttttttgaa gcgaagtttg atgcaggcag tatctattct atcaaaatcc
    21781 tgcggggata tgaattgaga ggatatcttc ttcaaccagc tgtttagtgt aacggtctcc
    21841 gtctggtttg atacatattg ccctttcatg gataattgta tacttggcga ttaaatatta
    21901 tatagaattc tattaaaaca ccgatacaaa tgtacagtaa atcaatcgac attatagata
    21961 atattgtaac aaaattaatt gaagctaaga tatatggaaa tcaacacagt aaaagtgata
    22021 ggcgggcggg gcaaagatgg tcaaccggag aaagtttccg attttgaact gaaaatgggc
    22081 gatatcgtca gcattgtcgg tccgaccggc tgcggaaaaa caaccctcat caacgacatc
    22141 gagctctttg ccaataacaa cactccatct gagaggcaag tcctgatcaa tggagaaccc
    22201 attccggagg aattctcctt cgatccttcc aaacacccga tagcgctgat ttctcagcac
    22261 acaaactttt tgtcggacct gccggtaggg gaatttctga agattcacgc tacgataagg
    22321 ggagcgaacg aaaccgacca aatgatttcc gaaacccttg cctttgccaa tcagcttacg
    22381 ggcgaggcta ttatccggga aacggccatg acggagcttt cgggagggca gacacggtcg
    22441 ctcctgattg cagacgcagt gatcatcggc aattctccga tcatcctcct ggatgagatt
    22501 gagaatgccg gtatccatcg taccaaggcg ctgaagttgt tgaagagata ccgcaaaatc
    22561 ttcgtttttg tcacgcatga tccccgcatc gcgctcctat ccgatttcag gatcatcatg
    22621 gaacacggtg ctatgcaaca gatcattgtt actgacactg aagagaaagt ggcggcaatc
    22681 ggactgaaga aaatcgatga tgtgatgctc gaattccgga aacttatccg gtcgggagag
    22741 aagatcaacg ccgaagtgtt cggagaacag atcagcgaac tggcagcaaa ttatcagatt
    22801 taccttaaca gaaacagctt atgagactaa ttatcattgc aggtccgcct acaagcggaa
    22861 agactacggt tatcaaacag gtgatccgga ggatactgaa aaaagggctc aaaccggcct
    22921 accttaagat agatgtgatg tttgccgatg aagatgagca aataaagaaa gaattttcaa
    22981 tcccggctaa aaagatttat tccggcgaac tctgccccga tcactgcaac gtggtggtcc
    23041 ttgatgaagt agtggaatgg gccgaaaagg aacaagccga tgtgctcttc gtcgaaacag
    23101 cagggttgtg cctgagatgc tctccctatg ttgagaactg cctgggtatc gtcgttctgg
    23161 aagcaaccag cggaatgaat ctcccaagga agattggtcc catgctcact ctggccgata
    23221 tctgtgttgt gaccaagata gaccttgttt cacaggctga acgggaagta ttccggtcca
    23281 ggattttgga tgccactaaa ggcacggcag tggttgagag taatgccctt tacggcatcg
    23341 gaatagatcc cattgtccgc gatatcctga aaaccgatcc cgttgaaatg cccatgttcc
    23401 tgcgcggcaa tcctcctgtc gggacatgca ctatctgtgt cggcaaaaag gaaatcggtt
    23461 caaaaaacca tttcggagtg ttgcggacga tggaaaacga attattctat gtcggagaat
    23521 agtaccgctg cgggaaggat aatgtcattc gtgcagtttt gatccgatac ggaaaaccat
    23581 tccggtgaaa ccgcataaaa cactgagaca gagaaggact caatgatact ggcctttgtt
    23641 ttccagctaa tcttcagact aattggggca tcgattccag atacgattaa aggggaatca
    23701 caccgtatgg ctaccacttt tacgaaacgc ccaacttctg tgaatatgac ttatctgaag
    23761 cacatgggtt ttgctatttg gctttccctg aattccttta cctgttgcat agtttccctg
    23821 gttcactcct tctttctctt tctctttgag acatacacca gaaggcagat tgcaaatatg
    23881 aaccagcatt aacttcctat atcaaagagt aaaaaacgac aaaatgaaaa gcgtatatct
    23941 tgacaatgcc gccacaacac ctcttgacga gcaggttctt caattgatgg atgaggttta
    24001 cagaaaaaat ttcggaaatg catcaagcaa ccatcggtac ggaacagaag caaaatattc
    24061 gcttaatcgc tatcgctccc ggttagccga aatgatacaa gcgaaacctg aagaaataat
    24121 attcacctcc ggaggaacag aatcgaataa tctggcattg aaaggagtag ccttcgccaa
    24181 caggcaccgt gggaatcaca tcatcgtttc ggcaattgaa cacgactgca tcctgaatac
    24241 ctgcaaatgg ctcgggaatc agggatttat catctcttac atcccggtcg gttccgatgg
    24301 aattgtagat accggaatac ttgaaaggat gatcaacccg aaaaccatcc tcgtatcagt
    24361 gatgcatgcc aataatgaaa ttggtactat ccagccggtg gaagaaatcg ggcgtatctg
    24421 ctgcgaaaaa aatgtccttt ttcactccga cgcatgtcag tcgtttggaa agataccggt
    24481 gaacgtggaa ggtatgcagg tttcattgct atctctcaat gcgcataaga tatacgggcc
    24541 taaaggagtg ggctgcctgt atgtaaggaa aaacactcca attactcctc ttttacacgg
    24601 tgggggacag gaattcggta tccggtcgac aaccgaaaac cttcctggaa ttgccggatt
    24661 tgtgaaggcc gctgaattat gccaagaaga aatggaaaat gaaccgaaac ggctcgcgac
    24721 attgagagat caaatgattg atgagttgca ggaagaaatt gagggtttct atgtcaatgg
    24781 cagccttacc cacaggcttc cgcagaatct gaatttcggc atcagcggtc ttgaagggga
    24841 aaccatgcga cttttgttgc tgctcgatga ggccggcata gccgtttcag caggatcagc
    24901 ctgttctaac aacgattcgt caaaaagcgc ttcccatgtc ctgcaggcca tcgggaaaaa
    24961 ccagtttgag gccagaggag ccgtgagggt tagcctcgga cgtttcaacc aggaggatga
    25021 tatcagatgg ttcctggatt cgctcaggca atgtatccga catcttaatc caatcttttc
    25081 gtaaccagtc atggctgaaa tcactctacc tcaccgctac cacaggcatc ttctggttcc
    25141 tgaaatcggt taccggggaa tcagggcgat catgagctca tcagtcattg tgatcggcgc
    25201 cggaggcctg ggctgtcctg cacttcaata tctggccgca gccggtgtag gaaggatcgg
    25261 tatcgtagat tatgatatcg tttcggaatc caatcttcaa aggcagatac tatatactcc
    25321 cgccgacctc ggatacctca aggcagatag ggctgccgca aggctgaaag agttaaatcc
    25381 gctcgttgat atttatacct atcctgttaa acttaacagt aacaatgcac tggagattct
    25441 tagggactat gatgtcattc tcgatggatc ggataatttt ccggctcggt tcctggtgaa
    25501 tgatgcctgc gtaatcctgg acaagccttt tgttttcggg gcactatatc agtttaaaag
    25561 ccaggtttca gttttcaatt atcagggagg gccaacatac cgttgcctgg tcccggaata
    25621 ccctgacagc tcagaatcat tgaattgtga tgacgtcgga gtgcttggaa cggttgcagg
    25681 gatgaccggt tgcatcatgg ccggggaggt catcaagatc atcatacagg aagggaatgt
    25741 cctgagcggt aaactgatgc agatggattg cctgacactc caggtggata tcttaacaat
    25801 caaaaacaat tctgaactgc ccaaaataca ggaactgctg gattatgagg agctctgcaa
    25861 tgcgaagttt gatcaatggc cggtcagatc cattacacct gcgacgttgc gactattgat
    25921 gaatgaaaat ttcagcggac aaatcgtgga tatccgggag cctgaagatt tttcccggtt
    25981 ttcgataccc ggaagcatca atcgctccgc atctgaaatt gaaaccaatc cggatctgat
    26041 atcgggtcat catccggtca tattcgtttg taagcatgga atcaagagtg agaataccat
    26101 tcgaatactg atccgaaaat ttggtttcag caatttgctg aacctggagg gcggtatttg
    26161 cggctggctt cattaaaaag aaaattaatc aaccctatat ggaaacaaat cagttagaaa
    26221 gtaaaacgct cgcattatta cccaaattgg actgtggagc atgtggatac aaatcctgca
    26281 acgacttcgg cagagccgtc gataaaggcg ctgaggcact gtcgaagtgc attcatgtca
    26341 cctttcaatc ggaaatggct aaatcagcta ccggatgtca ttcctgtgca gccgaccagg
    26401 ttggcattgg tgagaaaaac ggatggaaag acagcctgaa ccgggattat gattttatcc
    26461 ttgatatttt cccggatgaa ccggggccga aagagaccat tcttccatac aatcctgcat
    26521 tggtaaaaga gctgggagta aagaagggtg atatcatgat cgggcgtcct atgggtatgt
    26581 cgtgcggatg ccccataacg cattgcggcg ttgttaccgg tgtggatgcc cgaaacggcg
    26641 taatattctg gtgtgtcacc ggtcctttac ggcccaggac cgaaggattt atcgatatcg
    26701 gatattatgt ttcccaaagt tatgaaggca tcatcagaga gtggaaatca gatatccgtt
    26761 tgggaacccg ttactggttc ttaccccgcc gatgcatgct tcaatggagg cacagcggct
    26821 tggtgaatgc cataaccaaa ctgaaggacg gaagttataa ggttaggatt gaaggattgt
    26881 tgattggtta atgagctgcg ataatctgaa ctcaaccatc tacaaacaat ggttgattca
    26941 ggaagacagg gattcgggag tttattttta aatcccggtt cctgtctttg gtaatcatac
    27001 attttgaagt ccggcaagag gattttgtct gaaagtatgg agccgttcag ttcctgatat
    27061 ataggataag acagccgact cttttagcat gttttacttt gcctgctgac agggaatcat
    27121 agacctgctt tgaaccggag tatcggatgt ggctccttaa aggccgaatt attctaaagc
    27181 aattaaacct ctgcaaatca aaagtgctct tataatttta aagtcagaaa tcggaattct
    27241 gcccgtctta tcattatttt ccaggatatg cctttaaatc aagccaaaat cctgtaattt
    27301 ccatgatcgg cagaacacgc agaatcatga tgaagtgatt tatctatgtt agcaaaaata
    27361 aatacaaata attaaatttc aaactcttag atcaggattg cagtatctgg agtccgaaat
    27421 aaacactttt tcagccagcc ctttcacttt tatcccttcg taaatattcg aatcgcaacg
    27481 ctggtactgc tctttggccg aaatcatcca ttccttatca ggattccaga tgaccaggtc
    27541 ggcatcaaat cctgctgcga tctttccttt ggtatcgccc cagccgaaaa tcgccgcagc
    27601 acgatgagaa gtaaggctca caaactgatt tagcgaaatt ttcttttcca ggacgccgaa
    27661 agtaaacaac aggcttagac ggaattcaat accgccggcg ccgccgggga tcttcgtaaa
    27721 atcgttgatg cccctgtctt tttgtccgtg caggttgaag ggacaatggt cggtggatac
    27781 ggtgtcgaag gcgccccgcg acagttcatc ccacaatttt agcctttccg gttcagggcg
    27841 caggggaggg gaaatcacgt aaggaagaac ctgccggtcg ggcagggatg catcatagac
    27901 gctgttattg agtatcaggt attggataca ggtttcgccg aagaccttca gccctttttc
    27961 ctttgcttcg cgtatggccc cggcgccttc gcctgtggat acatgtacga tgtacaccgg
    28021 gcatcccgtt ttttccgaca gatcaattac cttcctgatg gcgttcactt cggcagcaac
    28081 cggccgcgac agggcatgaa aagccgcccg ggttttgcct gaggcgatat attgtttttg
    28141 gaggcggctt atcatctcgc cgtcttcgca atgcaccagc acggttccgc cctcgggacc
    28201 tgccacccgc atcagttgtt ccaattcatt catgctgatg ccgatggtat cacgataagc
    28261 cagataggtt ttaaaagaag taatgccttc gtaacggatg caacggcgta cttcctccga
    28321 aacgcgtttg ttccattcgc tgatgcccat gtggagcttc cagccgcaca gggattccct
    28381 ggcttcggtt cgccgcaatt caagcgcttc cggcagggat tgcccccgtt ccggggtgac
    28441 gaagtcgatg accgacgtgg tcccgccggc cagcgccgct ttgctcccgg tcacaaaatc
    28501 atcagaggaa caaccggcag gagtaggcaa ggcaaaatga acatgcgggt cgatgccgcc
    28561 ggggataaga tatcttcctt cggcttctat gactttaaaa tccgggaaat cagcgggatt
    28621 catcctgcca acggcttcga tttttccgcc ggatatagcc acatcagcct gcatcgattc
    28681 atcggcattg acgaccgtcc cgttgatgat taaatattta tcggtcttcg gcatgaataa
    28741 atgacttttg aagtttggtt gaattgtggt tttctgataa ttataccccg ttttcccgga
    28801 aggcgctgtc tctcataaga tgcttcccgg attcctcagc tttccatcct tttccagagc
    28861 cttcccgcct gacatcgggc ttctttcatg atctccgttt catctacggt gacgattttc
    28921 ctgttctcaa cgatgacttt tccctgcgaa ataacgtgtt caacatgccg tgattcaatc
    28981 ccgaagagga aatgcccgaa gaaattatcg ctgccgaaag gggtcggcgg atcgtaatcc
    29041 agcaccacga ggttgttatc cccatcgccg tcaaaatggt tttcatccag gtaacgatga
    29101 acattccgaa aacgccggca ggtttcttcg aaaccggggg cgtcggaatt ctttccggca
    29161 aggaaggctg attttgcact ttgaagcata tcgctgtgca tgccgtcggt tccgagcata
    29221 atgcgatctc cgagtccctg cgcatcaaat aagccgacgg aattattcag gttgctttcc
    29281 atattttcaa ccacccaggc cggggaaccg gcaaccagcg ccctttcatg gtcatcgagg
    29341 tgaaggcaat gagccagaat ggtcttcggg aattgcagga caccggcccc ggcaaaccgt
    29401 tcgataaccc tttttccgta gtttttcaaa cagtgttcct ggtcgctccg ggcttccgcc
    29461 acatggacgt gaatgcccga accggtttgt tctgccaatc tcacggcccg tttcagggtg
    29521 tcgtcgccaa ccgtgaaaga ggcgtgcaac ccgacaagcc cctggtgacc gctgaggaaa
    29581 gctgccgttt cattcagccc ctttgcggcg atatccgtgc cgtcgcggtc gctgatctca
    29641 taacagggaa ggagcccgac gcctgctgca tcaaaggctt cagcaatgat ttggaggctg
    29701 ccttcaacgg caaaaggcga agcatggtga tcgatgacaa acgtcacgcc gtttttagcg
    29761 caggcgatgg cagtgaccat ggcgcaggaa cggattatat ccgcgtcgag ggctttatcg
    29821 agtgtccacc agacgtactg caatatctcg ttgaaactgg caggttcctt tttcggggcg
    29881 cccattcccc gcgccaatgc cgagtaggca tggtgatgcc cgcaggcaaa ggattttgtg
    29941 atatattttc cggaacagtc aatggatcgg ctcccggctg tctgatcggg ggcaggaccg
    30001 atgatgatcc ggccgttgat tccctcttca accagaacgt ctttccggat gaagtccggc
    30061 gtttgccggt tgataaaggt gccgtttttg aggagaagca tgggggaagg ttaaaggtta
    30121 aaaggcaaaa gtcaaagggc aaaagtcaaa aggcaaggag caaggagtag ggagcagggg
    30181 gaagggagca gggagcatgg agcaaggagc aaggagcaag aaggacggag taaatggcta
    30241 aaggctaaca gctaaaggct aacagctaaa ggcaaacagc taacggccaa cagccataag
    30301 gtcaaagatc aaaggtgcaa catagatgtt ttaggattga acatatgccg gatgtctgaa
    30361 acaaggggat gtcaataata attcgtatcc acgatgggtt gccacaattg ccggtagcct
    30421 tcatcctgaa agcctttaca tactttttca aaacggcagc gggtgtgttc ctgcagctcg
    30481 acaatttcga aaaaagtaca gggctgtttt tcgcaacggc ctgtccggca tagcggttca
    30541 taattgcagc aacttctccc cagcgaccag aaaaagtcgt tgagtttggt taccgcatgg
    30601 cctgatatgt cggagatgag acggaaagcg tcgatgcagg tatttctgat ttcgatatca
    30661 tccggcaaca ggattttagc gaccagcttt tcacgcaacg aactgtcgtt gacttcaaca
    30721 cagcccagcc gcatcaacac ccgctgcatg tggtagtcca tgatagggat gaagttgcag
    30781 gtgtccttca ctttgaccaa tctggcttcc tccagcaact tgatcagaaa ggtcaccttt
    30841 ttccttttcg gatcggaaaa cgccgccagg tctgaaagta tttcatagaa ccctttgccc
    30901 ctgaatacca ggtaaccatt ggcgttttga accagatact cgagtgatga ccgaaagttg
    30961 tcgccaacaa agcggtccag ttcggtaagc atgaaggctc gctcttcaag gcggtcgagg
    31021 cttgtgtttt caggtttttg atccggcgaa aacagccctg ccagaagcga ctgcaccccg
    31081 gcatttcctg ttcctgcaag gaatccgggc tgaagcagtt gatgctgatc tttcataagc
    31141 ccgctgaaga catgctcaat gtaatcccag ccgtagaggt tgagtccggg atgatgcagg
    31201 ttgtacgtct ggtggcagat ggctactgca taaaaatatc cgcgcaggcg aatctcttcg
    31261 ggagcgggga ttttcaggaa tggcctctca taaaaagttt ttctgggacg caattcctta
    31321 atcaacaggc ctgttttacg gcaaatttcc tcgttgatgc tgacagccgg tttcattgaa
    31381 ttcatttatt ttttcttttt attggcaatg tgtggcggcg gattccgtca aagatacaaa
    31441 gggcattggc gacggcggca gccgttggga ccagcccgat ttccccgatt cctctggcgc
    31501 cataggggcc aaccggatcc ggaacttcca cgcctttcac ttcaatttca ggcgtttggg
    31561 atgccggaag tacgccgcaa tcgcggagtt tcgtactcac cagcctgctg ccctccatcg
    31621 gcaaatcttc cgaaagggca taccccaagc ccatatgaac ggcgccctgg atttgtccct
    31681 cgaagagcat cgggttcatg attttcccgg catcatgggc ggctattact ttctcgactt
    31741 cgccgctgtc gtttaaaatt accagttgcg cggcataacc ataggaatag tgtgtgacca
    31801 ccttttccgc ttcggcgccg ggcttcgtgg tccagttgca ataataactg ccggtatagg
    31861 tgttcccggc gagttcttcg ggtggcttat gtgccagatc ggtgcgtaac gaagcggcag
    31921 cattgataat cgcattgcac agcagggccg tggcgcgtga agaggtagtc ataccggtcg
    31981 gaatttccgc acgggtatcc accgcaacac tgattagctg aggatcaata ccggtttcct
    32041 ggtaaagcgt ctggatggcc atattgtgga tgccttgtcc catctccgtc cagccatgtt
    32101 cgatcaggaa acgcccgcct tccagtaccc ggatcttcac atcgctgaaa tcggtcatcc
    32161 cgttgccgac gcctgaattt ttaatcccgc aagccagtcc ggcatattta gcgttgtaaa
    32221 aagaatcctt cagggccagc aggcattgcc ggatgcccac gccggcttcg agaacctgtc
    32281 cggtagccgt cattttgccg gtgtcgagcg cattgtcgta ccggaacttc caacggtcga
    32341 aacctccttt tttacaaagt tcgtcgatgc agctttcgag ggcgaaagtc acctggttca
    32401 ccccgaatcc gcgcatagcc cctgacggga tattgttggt atagatcgtc agcgtttcaa
    32461 tatccaccga cggaacatag taggcgcccg ttgcatggcc ggctacccgt tccatcactt
    32521 tagtgccaac cgaggcataa gcgcctgtat ctccggcagc cctgagtttc aaagccgtca
    32581 gtatcccgtc gcggttgcag gacaacgaaa tatccatccg gacggggtgg cgtttgggat
    32641 gcatgatgat ggactcttcc cgggcgagat ggagttttac cggcttcttc aggagccagg
    32701 caaacaggga aacatgtccc tggacggtca tatcctcgcg gccgccaaaa ccaccgccgc
    32761 aaggcacaag cgtcacatgc acctgttctt cgtcgattcc cagtaacgat gcaacctgtt
    32821 gacggtccac atagatgcct tgcccctggc tgaacagatg aaggccttcg ccttcgggta
    32881 atgcgatggc cgcttcggtt tcgagaaatg catgctctac gcgctgggtt tcataaaatc
    32941 cggataccgt gaaatccgaa ttctggaata tttcttctgc ctggtttccc cgtcgtacta
    33001 tgcacttttc aagcaggttg ggcttgtcgg gatgtacttg cggtgatccg ggttccaggg
    33061 ctttgtttat atccgaaacc ggttcaagaa cttcgtagct gattttgatc agcgcagctg
    33121 ctttgcgggc aacagtttca ttccgggcta ccaccccggc tatcacatcg ccgatatacc
    33181 gggtggtttc tccaatggca accataagcg gccaatcgct gaagatcagg ccggtataac
    33241 gatcgccggg cacatccgcg gcggtaaaaa tgcagataac gccttcaagc ttgccggctt
    33301 ctgccgtgtc gatctttaaa acccttgccc tgggatggtc gctgaaccgc agggccgcat
    33361 gaagcattcc ttcgaagtgc atatcgttga taaacttgcg tttgccgata gccgtatcat
    33421 aagcttcatg ttttaagaat ggtttgccga tgccggcctg taagggggga atttcaggct
    33481 tatttccggt tcgcaggttt tccagggctt cggaaatggc atcgatgatt tttatgtaac
    33541 cggtacagcg gcacatattc aggccgattg cctttttgat ctcttcacgc gtcggttttg
    33601 ggttttcgct gaacagcgtt ttcgtccgca taataaaacc gggagtgcaa aatccgcact
    33661 ggatggctcc tttacccacg aaggcgtttg caatcacatc ccgcacttct gcaggaatgc
    33721 cttccagcgt aacaacgcag gcattttcaa gggatttcat tttttgcgta caggaaagtt
    33781 ttgccttccc gttgatttcc accatgcagg cgccgcaggc cgcctgccct gaacagccgt
    33841 cctttacgga agtgatcctt tccgaaagtt tcaggtaatc cagcagggta cggtctccgt
    33901 caccgctgta tccgatcctc ttaccgttca gcgtgaatgc aatcatgccg ttttactttg
    33961 taaaaatctt ttcaagtgat gaaatttccg gctttacagg ctccggcaaa cggattaccg
    34021 gctgaacgga tttcaggtct ttgaggccgc tgcctgtcag caaaaccaca tttttcgagc
    34081 cttcaggaat ccttttttcc gaccggtatt tcagcatccc ggcaaaagcg gcggcagcag
    34141 caggttcggc gaatattccg gttgaatgcg caagactgac cgaggcatcc agtatttcgt
    34201 catcggtcac gataactgcc tcgcctttgt attgacccat gtaaccggcc gtcatgtgaa
    34261 agttggcagg aatatttacg gagatcgagt cggcaaaggt agaggagggg acagatctga
    34321 attcacggtt cccgaggttc ctgatcaggt tactgctgcc ttccgactga accgccacca
    34381 tgaccggcat atggtcgatc acctccagaa gcagcagatc ttcaaaccct ttgtacactc
    34441 ccgagatgat gacgccatcg cctacgggaa caaaaatccg gtcgggggcc atatagttca
    34501 gttggctgaa aagttcgaag gctacggttt tctttccctc gatggtgagc gggttatatg
    34561 cggtattccg gttatagacg ccgaatgctt cggtgacttt tatgctcagg ctgaaagcgt
    34621 cgtcgtaagt tccgtcaaca gggacaatgg tggcgccgta catgacaatc tgggtgagtt
    34681 tggccagcgg cgcggttgcc ggtacaaaga tcactgactt ttgtccctgt gctgcgcaga
    34741 tgccggccag cgacgagcct gcattgcctg tggatgcggt gatgatggtg ccgatcccgt
    34801 tctccttagc ccaggccgag accagggcag aagcgcgatc cttgaaagaa aaggtcgggt
    34861 tttgggaatc atcctttagc aggaggtcaa acggtaactc ttccccatca aagtgttcga
    34921 tatgataaag aggtgtattc ccgatcttca gttttggcag gctggcgcat ccgttgatgg
    34981 ggagcagggg tagaaaattc tgttcttcaa gtttcccgaa aaggctgttc ctgtatgtct
    35041 cctttatctc tttgtagtta taaacggcgc ttaaaacgcc tttcttcggt tgcccgggct
    35101 ggttttgccc cgagcatacg gggcacaggt aaataacctt attaccgggg tattcggccc
    35161 cgcagttgca acagcagtaa tggaattttt ctttcatccg gtgtacgggt gttttattga
    35221 aaggagatca tttcaatagt cttgtctgtt cgttgaattc atggataagt tcatcccagc
    35281 gggctggctg gctgaaaagc tgcggccaga tttgtctgtc gtcccacaac tgccgcaggt
    35341 cgtccacatc cttgccttgc tgttccaccc aggtaaagta tttcaggttg tggatggctt
    35401 tccggtcatt gtaagagagt tccttcatat aaccgacaga tgccccgatc atacattttt
    35461 cgtgatcttt ggccgcctgc atcaccgaat aatttccgcg ttcatcgttc agctctttaa
    35521 ggcgggaacg gtacatttcg gaggagtcgg tcgcaatggt cagcaaaatg tcgtcggatg
    35581 tcatcccgaa atatttggcc gttttaatcg ccgagagaat attggcgatt cctgaaatgc
    35641 ctatcatcca tagttttgcc gtgaattccg catcaactcc gtttcccgta aggtattcat
    35701 gtccggcttt ttcgttaaac agccgcaaca gccgcatgca gtcttcatcg tcgatggcgg
    35761 tgaccacatc ggtatttttc acgttatgga tccatgggat atgcttgtcg ccgatgcctt
    35821 cgatgcgatg tccgccgaaa ccgttcatga gcagggtagg gcattgcagg gcttcggaag
    35881 cgacaacctt aaggagggga aatcgtgtcc ggaggtaatc gccggcggca attgttccgg
    35941 cagaacctgt cgccgaaatg taagccgcca gttgtgaatt tttcccggca aggttcaggt
    36001 atacttcttc gagggcgtgg ccggttattt cataatgcca gcaactgttg ccaaactcat
    36061 caaactggtt gaagataata caatcggggc gggtgcaact gatttcgcgg cacttgtcgt
    36121 atatttcctt tacgttcgat tcgcagccgg gggtggcgat cacctcggcg ccgatttccc
    36181 tgagccaggt gaaacgttcg cggctcatct cctcggggag gatcgcgaca gccccgacat
    36241 ccatgaggta ggaatcgaaa gcgccgccgc ggcaaaaatt tcccgtcgat ggccagactg
    36301 ctttatgaaa agttgggtcg aactgcccgg taatgatgcg gggagccaga caaccgtatg
    36361 cagcacccac tttgtgtgcg cccgtcggga accatttacc caataatacg atgatccggg
    36421 catcgatgcc ggtcagcgac cggggcaatt cgatgtaatt cgggctgccg tataacccgc
    36481 cggaatcttt gggctcattt ttccaggtga tgcggaaaag gttcagcgga tgaagctccc
    36541 acaggccgat tcttttcaat tgatctttaa ccttgtccgg taccagttcg ggatcccgca
    36601 tctgggcaaa tgtcggcagg atgatgccct tttcacggta tcggctgata gctttctgaa
    36661 gaatttcccg gtttatgact ttatcggtga tttgtatcat gttttccaga gcgtttattg
    36721 ggtttatact gagtggccgc tgaccggccg gatggcatgg ctgatttttt tgattttacc
    36781 ggtttcagga acttttttca aaggctatca taccgtaatt tgatatttca actgcattgc
    36841 cccctgcatg ataacgctca tttcggcggc aaaggtgaac ctgcattccc tgacgcaggg
    36901 agtaaggaac cggacttctt tcaggctaaa atcgccgggg ttgataaccg cccagacctg
    36961 atcggtttca aagatatagt gattatcctg caaagtcagc gttttgacat gctcatggtg
    37021 cttgtaaatc agtacatccc tgccggggag ccggctcagc aaatagcctt cctcctcact
    37081 cacgagcgtt ggggccgaaa ggcagagtcc gggtttatgg cggtacggac ttccgctggc
    37141 cgggcaaaat gtatcgcaat tgccgcattc gttgcagaag tcgcggatgt tcagcacctg
    37201 ccggcgctga cttaccgtaa atacttcatc ggcttcaatc ctgatctgcc cgtcatgatg
    37261 cagtaccgct ttttgtaagt agaagtgaac cggttcgatg ctgtaactga aattggccag
    37321 attagggcac acgcttacac aggtattgca caattcgtcg cattgcaggc atcttgatgc
    37381 ttccgtcata gcttcggacg gcgtcaggga accgatcacc ggcttgaagt tctttctatc
    37441 atccgtcgga atttctttgg gcgaaatgcc gtaaatcctg gtggcttttt ttctgacgag
    37501 gtcatcgaaa ccggaactac ggacggatgc ggattcaacc cggaccggcg tgaggccgag
    37561 actgtccaga atttcagcag ccgctttcct gccgtcggca atggcgttta taaccgttga
    37621 agcgcctcgt atcgcatcac cgccaataaa gaccccttcg agggaagttt tgcagcttcc
    37681 ggggaatgtc ttgagattgg cagtctggag aaaccggata tccggttgtt gtccgatagc
    37741 cggtattacg gtgtcgcagg gaacttcgaa ttccgatccc gggactttga tcggcaccgg
    37801 acgtccgtcg acgcctttca tggcaggaat atttctggaa cagataatgg atgttaccct
    37861 tccgtcatgc gaaatgatac gttccgggtt tgcaagttcc attatttcaa taccttcctc
    37921 aagggcagcc ctgatttcac ttttgtcggc aggcatttcc ttcctggtgc ggcggtacag
    37981 gatggtgact ttgccatcac ggccggtgag cctgaaagcc gtgcgcgctg tatccatggc
    38041 agtgttgccg ccccctatga tcacaatgtt tttgccgatg ccggtcggaa tactttcttt
    38101 tacttcggcc agaaatttta acggatcaag gactcctccg gaatcaatac cttcgatatt
    38161 gagttttgcg gatgtctgtg ccccgacggc caggaagacg gcatcatggc ttttgcggat
    38221 agcatcgaaa ttatttatac ctacggtatg attgtcgtgt agctgaactc ctgatgccag
    38281 gatccgttgt atatcccgtt caacggcttc gccggttagc ctgaatgacg ggatcgccgc
    38341 ggaaaccatg cctcccggca cttttttctg ttcgaagata tcgacatcac agccggccag
    38401 cctgagatac caggcgcagg ataatccggc cggtccggcc ccgataacgg cgaccttttt
    38461 gcccgatggt attatctttc cggaatccgg ttccccgtgg tggctttcgg caataaaacg
    38521 ttttatatcc ctgatcgcca gcggctcgtc ataattgaca cgggtgcatt tcacccggca
    38581 gggttggtcg cagatcatac cagtcaccga aggaaaagga tttgtacgga agatagtatc
    38641 aaatgcttct ggaaaagagc ctgatgccgt gtgagccata tattccggga tgtcctggct
    38701 tgccgggcac gtatcggtac agggcgcatg aatgcaatca aagaaatcga gctgacggcc
    38761 tgtttttact gagaaatcag tccatgtgtt tttatgatag gcaggtgcgt ctgctacccg
    38821 gccggcatac cgctcaaggt tcagcagggc tgattcgtcc accgaaagat catcccgtcc
    38881 ggcaatattc ctgatataat ccccgatggt tccggctttg gcttcttcaa acgctgtccg
    38941 caaattagta aaatattgcc gcagccttcc gtatccgccg ggtttgagca ggtcgctgca
    39001 aacggtaacc ggctttagcc cgcatgacaa tacttcagcg atgttgaaac aatccacgcc
    39061 ccccgaaaag gatatatcca gctttccgtt gaagtcgttc tgaagttttt cggccagttt
    39121 cacactgatc ggatgcagag cccgtccgct catatatatc atgctttcct tttcatcgaa
    39181 accaggcttg ttgttgatgc actcgagggt gttggtcaac tttacgccga attgcagccc
    39241 gccggcatca gcggtggcct gcaggttttt caggatggtg agcgcctcgg gatatttcag
    39301 gtcatgatca aaggcggcat cgggaacttc ggcagtaaat ccaagttggt cgtttagcag
    39361 gttccggata tcctctttcc ccagtagggt ggggttgagt ttgatggtag tatgcagttt
    39421 cttttcccgg aggaggtatg aaccgatttt ttcaatttcg tcagggggac agccatgcat
    39481 cgtgcttagc gtgatgctgc cggatatgca cgaaggaatt tcgatgtggc cgatggcggg
    39541 ataaacctgc ctgatggaat gaatggcttt ggttagttcg ggactgcaat cctgcatctt
    39601 cgaaaggaac cactggacgt tatccttttt gatgccttcg agattgtatc ccgcactcat
    39661 attgaagatg gtgcccaaag gagcggtaaa gcccatttca tggcgaagga tatgaatgat
    39721 gatccaggca ttcaggtact cgctgaacga ttgtctgatt cccagttcct gcgaccactc
    39781 gcagttatac ccttcgtcct gcatgtcgat gcagggtttt gccacacaga ggtcatcgag
    39841 cgtctggacg gttttcagct cgatatagcg tgcgccgcaa agccaggcgg ctatgatatt
    39901 ctgcgccagc tgggtatgag ggccggcagc gacgcccaca ggcgtctcaa gccactgccc
    39961 gtaacgttcc atacggaaag gatcggcctg gccgggaata aaaaacaggt tttcgggcaa
    40021 tccaaggaaa cgatggcaac gtatctccga aagagtaaga gataaaagac tgggaagagg
    40081 aagcggtgaa aatttgtccg acacagtaaa ctttttagga ataagttttt atgtgtatca
    40141 aatttaggac aaattacata gggaaaacag atttgtacag ttattattga atatttggtt
    40201 taacagaact gattctgcaa ttccataacg ggagataagg ttccggagtt ctcccaagat
    40261 caacaggcgt tgagatggca ggcccttttc acactcggcc tgaagccggt atgtggcttg
    40321 aagggtataa ggttgacagg taacgtatta ccgttatttt ataagttcta tctcgtaacc
    40381 ggcttttttt atacctgcga ggatatcagc ggccttttcc ggatcgcatt ccaccgtaag
    40441 aagccgttca ggggctgcca gatcaacttt ccatgatgcg ataccttgga tgttgttcat
    40501 ggttggggtt atcgcgctga tacatccgtt gcatttaagg tttgtcttga atttcagggt
    40561 ttccattttc tttcagggtt taaaatcagg tgttttgtga tcgggctgca ggctgttagg
    40621 ttaaggtttc agagtttcag tgtttcagag tttcagtcct gcctgccttg cgcaaggctg
    40681 tgggaggggg cagggttcca gagttctgcg ttttgtgttt tgagttttg
//
LOCUS       DAOPYV010000024        40116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_856_length_40116_cov_11.932902, whole genome shotgun sequence.
ACCESSION   DAOPYV010000024 DAOPYV010000000
VERSION     DAOPYV010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 40116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40116
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_856_length_40116_cov_11.932902"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..1485
                     /gene="amrB"
                     /locus_tag="PKI35_04730"
     CDS             388..1485
                     /gene="amrB"
                     /locus_tag="PKI35_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNX43722.1"
                     /translation="MKRCFSPLLFLMIGYIVTGCNTSALQRGDNRVRPPADTVGFAHL
                     DRQIDSVMNRINRNYSKELPEVIIHTDSCWRVAICPHDDYAYASWQYPAILRNIKAKT
                     VLIFGVAHKAKQFNISDRIVFDSYPYWHGPYKNIRISPYREEIMASMPRQYYLVSDSL
                     QAAEHSVEGMLPFLQYFNRDIEIIPILVPYMDAATTEGISRSLSTAVWRVMKKHHLRW
                     GNDLALLITTDAVHYGDEDWGGKNMAPFGTDSAGYKKAVAREHAIIDSCLTGELNKER
                     IARFIHYTVQPGDYKEYKWTWCGRYSILLGLMTAINLREELNSAPLSGKCPAYNTSID
                     HAPLPVEDLGMGRTAIASLRHWVGYVSVGYK"
     gene            complement(1526..1657)
                     /locus_tag="PKI35_04735"
     CDS             complement(1526..1657)
                     /locus_tag="PKI35_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43723.1"
                     /translation="MNTSAIILWLSVSAIVTGLCGYFFFKVLSVNKKKEPDSYSDNE"
     gene            complement(1654..2937)
                     /gene="nhaD"
                     /locus_tag="PKI35_04740"
     CDS             complement(1654..2937)
                     /gene="nhaD"
                     /locus_tag="PKI35_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HNX43724.1"
                     /translation="MEIIIALIFVLGYIFIILEHKLDLNKAASALLTGVICWAVYVLM
                     SADSHLVNEQLLEHLGEISGILFFLMGAMTIVEVIDAHDGFEVITRMIKTTSKRKLLW
                     IIGLLTFFLSAVLDNLTTTIVMVTLLRKLISDKNERMLFTGLVIIAANSGGAWSPIGD
                     VTTTMLWIGGQVSTWKIIVKLILPSLVSLFVPLIAFTFMLKGNTRKLPVINASGNPMA
                     STFERNLFFFAGVGALLFVPVFKTITHLPPFMGMMFGLGVLWLLSEFVHKDKNEEEKS
                     RYSVRHALHRIDMPSVLFFLGILIAISALQSSGMLSHLAVLMEEKIRNINLVVLSIGL
                     LSSVVDNVPLVAAAIGMYDLQVYPTDHYFWEFLAYCAGTGGSALIIGSAAGVAAMGME
                     KINFFWYLRKISLFALLGYFAGAMVYILQHQMLGL"
     gene            complement(3406..4593)
                     /locus_tag="PKI35_04745"
     CDS             complement(3406..4593)
                     /locus_tag="PKI35_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNX43725.1"
                     /translation="MDIQVIVFICLLLLLSYLFDISSARTRIPSVILLLFLGWIVRQT
                     TIMLKISIPDLNPVLPVLGTIGLILIVLEGALELEINMAKVGVIGKALLLALIPMLLL
                     LFVIAGVFYFFQVVEFKTALLNALPFCVISSAIAIPSVRNLSPIKKELVIYESSFSDI
                     IGVLLFNFIAINNTFNGHIFFRFGSQLIVIVIGSFILVLMLSFLLSRISHHITYTPII
                     LLVILIYAVSKQFHLPGLIFILVFGLFLGNTEEFKRFKIFEQFRPQKLEAEVVKFKDI
                     TYEVTFLVRSLFFLLFGYLMETDELLDLGSLPWSCGIVISIYLIRWVTLKVFKLPATE
                     LLFVAPRGLITILLFISILPDQRMPVVGKSLVIQTIVLSVLVMMFGLMLSGRPTKEIE
                     KKE"
     gene            complement(4669..5262)
                     /locus_tag="PKI35_04750"
     CDS             complement(4669..5262)
                     /locus_tag="PKI35_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNX43726.1"
                     /translation="MSTINLLIHNFLILFAMVNAIGNLPLFADLTAQMEKKEKSRSFL
                     VAVLTGGSIVLIFALFGDFMLRNVFVVSTSAFKIAGGLLVFAVAAKGVLSGQVSGEVQ
                     NRPSSDVAVFPMGFPFLAGPGTILTTILLLQANGYIITVSTILLVYAAILPLLYLAPW
                     VEKIIGKIGVMVIARILYIFICAKGVSFVLDGFHVKI"
     gene            complement(5259..6608)
                     /locus_tag="PKI35_04755"
     CDS             complement(5259..6608)
                     /locus_tag="PKI35_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNX43727.1"
                     /translation="MAGIAGVLKSGQKELVEKMLGIISHRGEYGKEIIETNEVTVGMI
                     WSLHENDHVRNLFDQNIFRIGPGYGHHVMVTADDGNVRIERGRVGVVPLYLGTIDNET
                     VFASEVKAILPVTDNIVELLPGKAISNNKIVTYHELKRGEPLTDGPLEIAEQLKELLQ
                     KVVARRINSGTAGAWLSGGLDSSAIAALAKPNVNKLYTFAGGVKEAPDLRFAREAASF
                     IGSEHHEVVIDREMMLKILPDVIYHLESFDALLVRSSIINLCVAYAASNYVTDVFSGE
                     GGDELFAGYSYLKDIPPADLDSELIDITKRLHNTALQRVDRSASAFGLSAHVVLLDPE
                     VVEFALRIPSGYKITGGVEKWIFRKAMSGLLPDSILSRPKAKFWEGAGVESLISDYAN
                     RKISGRDFENERKLRNGWMLNSREELFYYRIFREHFGDLENLEWMGRTKGSPVMKEK"
     gene            complement(6637..8277)
                     /locus_tag="PKI35_04760"
     CDS             complement(6637..8277)
                     /locus_tag="PKI35_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNX43728.1"
                     /translation="MKTKLIMGAALGNCVHVAGLHHFLKIAESEGYETLSLGPAVSIE
                     RLIRALREKNPDIVAVSYRLTPEVAEGLFSQLKEELNKAAIPDIKLIFGGPFPVAEVA
                     RKSGIFEHSFDGTESVEQIRIYLQGGQGKVAGTIYADNLIDRIAQKYPYPLIRHHFGR
                     PTLEETVTGAQTIAEAGVLDVLSIGPDQNAQEHFFQPGLMNHEMDGAGGVPLRKPEDL
                     EAIYNASRTGNFPLVRCYAGTMDLMKWAEMSVRTIHNAWGAIPLCWYSVIDGRSQRTL
                     EEAIAENQSVMRWYAERGIPVEVNESHQWSLRDAHDSLAVTMAFLAAYNAKKAGVKKY
                     VSQFMFNTPPGTSAAMDIAKMLAKKEMISELEDENFEMIREVRAGIAHFSPVPAVAKG
                     QLAASAVTSLSLKPHILHVVGYSEGDHATFPDELIESCRIVHGVLRNVLHGFPDLTID
                     SSVLRRKNELKDEARVILEALKSPKLGKSKDAWSDPEIIAKAVRVGILDAPHFKGNPH
                     LCGNILTRVIDGAWYPIDFFSGEPVDEKARLSGILQRL"
     gene            complement(8294..9415)
                     /locus_tag="PKI35_04765"
     CDS             complement(8294..9415)
                     /locus_tag="PKI35_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HNX43729.1"
                     /translation="MKKLFEVLEKPVIAVLGAGHGGLAMAGHLALMGYEVNLFNRGEE
                     RLWGVKSSGGIELTGEVSGFGPLKVITTNMEEAISNANVIMVVVPAVAHRYMAENVAP
                     CLKDGQMIILHPGRTLGALEFRQVLNSLKVKADVIVSEAQTFIYASRAIGPSQVKIFG
                     IKHSIPVASVRAHLIPVVISQLRVFYPQFVPGDNIFKTSFENIGSVFHPALCILNSGW
                     IEDDAEFQFYHEGATESVVKILEKTDAERIAVAEALGIQAISARQWFYMAYSTAGNTL
                     FEAMRKNPGYRGIMAPRTLKMRYLEEDVPCSLVPIASVGRMFKVPTPTIDSLIHIASL
                     LNGVDYWAIGRTVRRMGIEGMTLRDIRLMAIGEPVKSHI"
     gene            complement(9697..10185)
                     /locus_tag="PKI35_04770"
     CDS             complement(9697..10185)
                     /locus_tag="PKI35_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43730.1"
                     /translation="MRKFDEVEYAGGGSEKMLVMFTGNSRQSWIGQFVKGHNQGLCKV
                     LTCSDSPFSLVVAGGKGYFVNIDKKEIKRFPDDGKTILSAVKSGRPECCIAGTETSLC
                     IIDHEGTFHEIVPEFDVEGFYLNEKEKSKVCGNLMSEINLCEKEIPFRIDLDSLILHV
                     EY"
     gene            10381..11070
                     /locus_tag="PKI35_04775"
     CDS             10381..11070
                     /locus_tag="PKI35_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX43731.1"
                     /translation="MSKARILVVEDELRLAKVLKKQIEEAGFSVQIANDGYTGKRYIE
                     ANKYDLAILDINIPIINGYDLCKEIRRIDFSIPIIMLTALGTLDNKISGFDAGADDYV
                     TKPYEIRELLARINVFLKRSDVDPSSHILSVADLRMDLVNKTVTRAGEKIDLTVKEFS
                     LLELFMKNKGKLLSREFIINKVWGIDFDPGTNVIDVYVNYLRKKIDKDFDLKLIHTKF
                     GFGFYLNESEI"
     gene            11077..12435
                     /locus_tag="PKI35_04780"
     CDS             11077..12435
                     /locus_tag="PKI35_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX43732.1"
                     /translation="MNIKVRLSFQFMLIAATVLVFFALLVYYFTFTTNREKFRNNLLN
                     RAKNTAIMLIDVKEVDSTLLKKIHQSTISWQSEEIAVTDTNLHIIYSNNINYLSNNVF
                     RSNISRDNHAYFTLSGKDGVFYRHVYKNKTVYTFVLAYDKARAVFLKELIQILFWSIL
                     FSLWLSVLFSYIFSKKAIKPISDIIDSVKAINSSNLGKRLHEGNRKDEIEQLAMTFNE
                     MLFNLEIAFKNQQEFVSNASHELKTPVSVMIAESDYFLNNKHSDEEYRTHLSEMIIDL
                     KKFNTYINSLLDLAQTNAGKSILFKEERLDEILYDAVHEIKNRYHNRKIVTKIHFPEN
                     DNGLSIEGHPGLLVIAIKNIIDNACKFSDEVVNVDLKPADGTIKLTISDSGIGIPLNE
                     INDVFKPFNRASNARFKSGFGIGLSLVFKIMEIHHVEMEVFSEENKGTRFELTFKRSA
                     TE"
     gene            12580..13305
                     /locus_tag="PKI35_04785"
     CDS             12580..13305
                     /locus_tag="PKI35_04785"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNX43733.1"
                     /translation="MNPYSWFRIVYFTLVPIAFGNCKGQNRTEEDYTRLRKEMVEHQL
                     KRSGIHDAKVLEAFLKVPRHIFVPVEYRDQSYDDTPLPIGYNQTISQPFIAAFMTEVL
                     KPDKSMKVLEIGTGSGYQAAILALLCKEVYSVEIIDSLTVRARKILEKEGFKNIKIKT
                     GDGYLGWQEYAPFDAIIVTCAPADIPKALADQLAEGGRMIIPTGGSYNQKLYLIEKIN
                     GQIRQKETLRVLFVPMIHGENRQ"
     gene            13512..14432
                     /locus_tag="PKI35_04790"
     CDS             13512..14432
                     /locus_tag="PKI35_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNX43734.1"
                     /translation="MKRRDFIAGAAAAGLIGTGRFAFGNIEQFAPSAPVLPYDLVAVR
                     GGEPEVMFDKAIQSLGGMKAFVKKGQKVVVKPNIGWDSSPERAADTNPNLVKRIVKAC
                     YEAGAKQVYVFDNTCDNWTNCYKNSGIEKAVKDAGGTMVGGNSKSYYHQVNIPRGKRL
                     KQAEVHELILDSDVFINVPILKHHSSSDLTIAMKNLMGVVWDRGYWHRNDLNQCIADF
                     ATWRKPTLNIVDAYRVMLRNGPKGVSASDVITMKQLLISTDIVAIDAAATKIWDPKKD
                     PAKVKHIALANELKAGNMNLAQLRINRIKL"
     gene            14539..15999
                     /locus_tag="PKI35_04795"
     CDS             14539..15999
                     /locus_tag="PKI35_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNX43735.1"
                     /translation="MHRAATWLQFGPSLVQFIHFAGLFSAGFLFIAILTLLFGRVYCS
                     AICPLGIFQDILIRIEKWFRKKKILRYRKPGWWRYVFLGMAVLSLLVGFMALFNLLDP
                     FSNFGRIFSSLGKPLAILINNAAAAISEKSGLAVINRYPIKGFDVATLIFPFLVISLL
                     IFLTWKHGRLFCNFICPVGTVLGLFSRFSLFSISLDKTKCNQCGKCAARCKAGCISIK
                     TQEIDHSRCVMCFDCIGPCPEEGVTFGLSSFKRSSQVATGKKASDSRRKFISSAFLLG
                     LAGRLAARENSGDTFERQTLIPEKKASPVSPPGSRSIEQFISTCIACHECVSACPAQV
                     LQPSLLQYGLQGFMQPHMDYHASFCNFECVVCSEVCPTTAIQPITVEEKKKIQLGKAV
                     FIKDNCVVSTFNTACGACSEHCPTKAVNMVPYLDGLRIPEVNQSICIGCGACEHACPT
                     KPYKAIFVEGNPVHLTAATPKEDIIERNVEEEEWAF"
     gene            16229..16753
                     /locus_tag="PKI35_04800"
     CDS             16229..16753
                     /locus_tag="PKI35_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43736.1"
                     /translation="MNTAIIPQYRFFSIRSLVSDAIALAFICLVPAISHLSGVPVYFI
                     EPMRLMLILAILSSHKANAYALAIILPLVSFLVSGHPEPLKMAVIMAELLVNTWLFYL
                     LLARTAKPFISMLTSIVVSKIFCYTLYLVVFSLAFVKAEAGTVFLVSQLLVTLLLSVI
                     TYLFLSAKQQKSKN"
     gene            16993..17850
                     /locus_tag="PKI35_04805"
     CDS             16993..17850
                     /locus_tag="PKI35_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43737.1"
                     /translation="MKNRSSGITLILILTATISFAQSYEARWQKTPVMADGISSEWSS
                     PLRFSDTKSGLQYAVTNDDKNLYICVKATGTATRMQILTSGMKIWIDANGRKKYPTSI
                     YFPMPLSRGEMKPDNSGLQQGRPGGERPSGKPDEMDFRKMFKNQGLKITLAGFKTGCD
                     GTFNIGESTQIKAAMDRDESNALTCEFIVPIGSFYSNDWNSFKEPPLIGMKISVDEMP
                     GNGQGTQRPEGGPPGGGPGGFPNDMDGGPGGPPDGEMGDRPSPPSGITGTSQEEISIK
                     FKIRLSKPE"
     gene            18116..19441
                     /locus_tag="PKI35_04810"
     CDS             18116..19441
                     /locus_tag="PKI35_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX43738.1"
                     /translation="MKLRISKLFILLISLSALQAEAFCQEKPAVWNLRSCINYALANN
                     IQVKKMQLGVKSSEADYDQAKASLWPGLNAGISQSFSNKSSINGSGESASLSGGYSLQ
                     SDVTLFDGYKLRNTIRQQEMMVKSNQLSVAESENSIQLAVTSAYLQILYARESVIDAE
                     NTLKSSEAQLERAKILYDAGSIARSNLAQVQSQYGNDKYSLVVARNNLNQQILSLKQL
                     LELDISQEIEIYFPEIGDDQVLKLIPSKEEVYNTALKNLPEVANSQLNVDIARKNAEI
                     AKAGLYPSLSLSASAGTGNSTAYSDGFVTQLGNNFYQNAGLSLSIPIFNKKQARSAIA
                     KAQIATETAQLGLAETEKNLLKEIETVYLDAVSAQSRFQAANEQKEYALTSYTLVEDQ
                     FNLGMKNTVDLITAKTTYLQAQQECLQAKYAAILNYKLLDFYQQKEIEL"
     gene            19461..20729
                     /locus_tag="PKI35_04815"
     CDS             19461..20729
                     /locus_tag="PKI35_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX43739.1"
                     /translation="MKKKSLIITGILVLIAAAAAWFLFGKSGNQQVIRYETAPVQIGT
                     IANTVTATGTVEPVKQVEVGTQVSGVIKNIYVDFNSQVKKGQLLAELDKTPLLAALAS
                     TKAELASANSQLVYQQANFNRIKSLFDKKAVSETDYETALYQYSSAKASVDKMKYEVE
                     RAQTNLGYAMIYSPIDGVVLNRAVDEGQTVAASFNTPTLFTIAQDLTKMQVVANVDEA
                     DIGQVAEGQKVSFTVDAFPSDLFEGKITQVRLEPTTNSNVVTYSVVVDAPNPDLKLKP
                     GLTASITVTVKEAADVLTVPAKALRFQPDSSLLANNSVKTVVNQASAANLPANNPSQA
                     EKQKDMPSAVNEDETPGTIWVKSGGSIHPVQVVSGISDETNVEIKSGLKEGDLVVVSM
                     VKSSAPAGKDTTEQSSSPFMPKRPGSERKK"
     gene            20812..21564
                     /locus_tag="PKI35_04820"
     CDS             20812..21564
                     /locus_tag="PKI35_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX43740.1"
                     /translation="MNEIIKATNLKREFLVGIEKVAALKGISFTIHGGEFVTIMGASG
                     SGKSTLLNILGCLDTPTSGEYLLDGTSVRAMSKNDRAHLRNHKIGFVFQTYNLLPRTT
                     ALENVELPLMYNSGITGRLRREKAEHMLEIVGLKDRLYHAPNQMSGGQQQRVAIARAL
                     VNDPVIILADEATGNLDTRTSYEIMALFQDLNNRGKTIAFVTHEPDIALFSSRSIVLR
                     DGRIVKDEKLSSPASAAEALASLPVETEEIEI"
     gene            21582..22802
                     /locus_tag="PKI35_04825"
     CDS             21582..22802
                     /locus_tag="PKI35_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX43741.1"
                     /translation="MNITNLFRISLKALHSNKFRSLLTMLGIIIGVASVIAMLAIGQG
                     SKQSIQDEVSKMGSNMLMIQPGADMFQGARMAASNTQTLILSDYEAIKKQCANLSAVS
                     PMVDASGQAIYGANNYPVSMNGVNADYLQIRKYTIESGRMFTDKEILGSAKVCLIGQT
                     IVEEVFGNGVDPIGKTFRFGKLPFEVIGVLAEKGENGMGQDQDNIIIAPYTTVQKRVK
                     AITYLNGIFASTTNESVTEQAISEIESILRTRHKIAAGDDDDFHVRSQQEMLTMFSST
                     TRVMTVLLAVIAGISLIVGGIGIMNIMYVSVTERTREIGLRMAVGARGIDILAQFLIE
                     AVMISVVGGIIGVALGIGSSFVIENVMKWPVAITSYSIILSFVVCTFTGVFFGWYPAR
                     KAANLDPIDAIRYE"
     gene            22851..23903
                     /locus_tag="PKI35_04830"
     CDS             22851..23903
                     /locus_tag="PKI35_04830"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNX43742.1"
                     /translation="MTVYDKKYSLQILIHCIVWVIVITFPLIFIYTEGEKDLVRYLDF
                     TLPIIMMMIVFYTNYLFIIGKYLFNKKIWQFFLINAVIFAVSLVMLELMKQYVWDHYF
                     SFQPPRHPGPPKKDMMRILFYRNLISLVCTLGVATAIKMTALWYNKMAEQKEKEKLHI
                     ETELASLKQQLNPHFLFNTLNNIYSLISINQEYAQDAVHRLSSLIRYVLYESDHPFVP
                     LSKDLIFTANYIQLMSLRLPPHVKLETDIMKPPESDTIAPLLFMSLVENAFKHGVSPE
                     HASYISVKIANKGHHELVCRVENSNFPKQESDRSGSGIGMNNLKKRLELIYPGAHTLD
                     IAEDNERYIATLTIKI"
     gene            23900..24610
                     /locus_tag="PKI35_04835"
     CDS             23900..24610
                     /locus_tag="PKI35_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX43743.1"
                     /translation="MNLRCIIVDDEPLALDLLEGYVVKTPWLILEGRFSSAFEAMAFL
                     EKVKVDLVFLDIQMPGLSGLEFSRSLQNGPRVIFTTAFGQYALDGFKVEALDYLLKPV
                     SYPEFLSAANKARRWFELTGKSAGQLARNTIFVKSDYKMVQIELSKILYIEGLKDYVK
                     IYREDTEKPVLTLMSMKSLEENLPENIFMRVHRSFIVNLEKITTIERNRIVFGKTYIP
                     VSDNYKDKFLKFVNERFF"
     gene            complement(24701..30562)
                     /locus_tag="PKI35_04840"
     CDS             complement(24701..30562)
                     /locus_tag="PKI35_04840"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX43744.1"
                     /translation="MKHRILLALLITALFSAANSFIKPLSAQPITVDFSYSGECVGSP
                     TLFTATVPDPSIITSWVWNMGDGTFESGQSISHTFAGATIYQVILTVTDINGNTGIFT
                     RNVQIYGLPTPLFSYETPNCQADSVHFTDLSSAPLDYITTWIWDYGDGSPNDTIHFPD
                     DPNADHIFPNPTTYNVTLTVINSRNCINSITIPVTVMSSPIANFHYSQTGLCEDQVIQ
                     FTDASSAVGSGNNVAWSWDFGDPMSGNNNSSNLENPIHTYNTQGTYTVVMTVTNFNNC
                     SDTMTKQIVIHDHPAAEFTWTSACLNDLVYFNPDTNAMNAGAISTWWWDFGDGGTSPS
                     KNTAHSYIAPGDYTVSLTVTDTLGCDSTISHLITIHPLPTAHFDPGSNNCSGATVNFT
                     NLSSTSWSYIWRWIWNYGDGTIDTVDLSGNPNVQHVYALPGNFLVTLTIYTADSCSNF
                     ETQTITIYPNPLANFDYDHNHACEGYPLQFTDLSQSNGGGSILGWNWDFGDPLSGTAN
                     YSTIQSPAHNFSGPGTYPVTLIVETSNGCSDTTSINVTVNAKPAVAFTSQNNCLSNQV
                     LFMADTNIVNTDAITNYAWDFNDGSPVFNTASPNTSHLYSTSGTYYVTLTVTDTNGCT
                     NFVTNPVVINPAPVSNFTWDQPACKDSEIQFTSLANLGGAPGYIVQWVWNFGDGNTTT
                     INHPGNPNISHTYTTFGNFNVTLTIKTTDSCTATASRLVTVSANPLANFSYDAIHCQG
                     EEIQFNDLSQPGSGGLQSWLWNFGDPGSGTANTSSLSSPTHTYNAAGTYTVTLEVANT
                     GGCTDTITKTIVINPHPTVDFSIEPGCVNDSTHFISTTYVNAAATNSWFWNFGDTYTS
                     VEEDPYHIYSASGSYQVTLTITDTAGCENTITHTAIVSLPPEASFLVSQQTCANLPVS
                     FDNTSTTSSGTIATWYWEFGDGSDTLITAPANPDITHVYSTAGTYTVTLTVNTSLGCE
                     DETSKTVIVKTSPLAAFAYENTCAGVAVNFTSQSATSGGSAIIGYLWNFGDPTSGVNN
                     TSILQNPLHIFNSAGTYTVSLQVTNADGCYDTVTQAVIINPSPALEYNWSNTCAGTAT
                     QFTVDETITMVAAVNSWDWDFGDGSAHSNEQNPLHTYAQYGNYNVVLTIVDSAGCSNT
                     KTHAVTINAQPVALFSFSSGCINSATQFSDESYTLNGEPIAVWHWDFGETAVANDTSN
                     LQNPQWTYTVQGVYTVTLTVTTAAGCQDSTSTTIQVFPNPTANFIYTPAPCENGAVYF
                     QDSSYASQASIVSWLWEFGPGSYSQLQNPVYVFYGSDSTYSVQLIVTDNRGCKDTIVK
                     SVYVPAAFDFTFNYTPTCIGDYMEFSPELIAPLTDSLVFFNWNFGDPGSGIYNNSALR
                     NPTHLYSEVGVYTVSLEAIDINNCNNTVFIPVSVEPLPLPDFEFTVGLCDTTAVFNDL
                     STGNGSDISEWIWTYGDGLSDTIYAPGNPDVTHKYLVPSLYTVELTVINANNCHNVFS
                     QEVLVRPCLEAVFSEEDSLVCQNYNLSFADSSYCGQPITEWYWDFGDGTTTTYNSYTS
                     PVNHIYTEAGNYSVLLRVTTAVSGQSISDSSIVDLIVSPSPVAEYSIGKVCYGTTAEF
                     TNETVNNGVIVSGYQWDFDDPASTEDTSTLRNPSWLFGAPGYYDVRLISSNTLGCSDT
                     VVKTIPVYMLPEANYDYSLSCAGNRTLFFDHSDSAYAPLSSWTWIFREGTELLDMKLE
                     QNPEYVFQNSGEHQVSLIVQDTNTCTDTVTQLVNTYPPPVSDFTYNANYENVQGQLQF
                     DNISVGATHYYWDFGNGETSYAEEPFVLYNLDGEYPITMIAYSDMECSDTARYVYNFM
                     VKGLFIPNAFSPENPHAEVQLFKPVGINLKEYLIQVYDRFGNLLWYSDKLDDQGRPAE
                     GWDGRYNGLLLQQGVYMWKAEAVFRDGVIWNSHDVGSYEGMKNSTHGTVTLIR"
     gene            complement(31044..31256)
                     /locus_tag="PKI35_04845"
     CDS             complement(31044..31256)
                     /locus_tag="PKI35_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43745.1"
                     /translation="MGDDIRFPIGSLFTILGAILTIYGGVTGNAEIYKASLGMNVNLW
                     TGLAMLIFGLWFLFMAWRKRNTAEKK"
     gene            complement(31269..32966)
                     /locus_tag="PKI35_04850"
     CDS             complement(31269..32966)
                     /locus_tag="PKI35_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNX43746.1"
                     /translation="MTENFSLNFIDYAIMAIYFAFVLGIGWLLKKYMKSSAQFLEGGK
                     SIPAWITGLAFISANLGALEVFGMGASGAKYGMLTCHFYLIGAIPGMIFLGIFMMPFY
                     YGSKARSVPEYLKFRFDEKTRGFNAFAFAIMTVFASGISMFALARLMEVLLHWNFHVS
                     LIASAVIVLIYTYLGGLKSAIYNEVLQFFLIVAGMLPLVIIGLNDVGGYSGLKESLGL
                     VAGAKGFEPDTWLHTWKYLGKPESNPLGVDWLGMFMGLGFVLGFGYWCTNFLVVQRGM
                     AANSMSAARRTPLIGALPKMFIPLIVVVPGMIAAAVMMMPDKNFQLIGGDPNDFNMII
                     PSMLVHYYPNGLLGLGITALMASFMSGMAGNVTAFNTVFTYDIYQSYINKGKSDQHYL
                     HVGQFATVFGVAVSILTAYVAASFNNIMDFLQLIFAFVNGPLFAVFLIGMFWKRATGH
                     GAFWGLISGTVAASLHHGLTLAEGKGAWIANIHEYYSSMAQSYWTAIFAFSVCFLVTV
                     IISLFTRQQKTREDLTGLVYSLTPRIKDEPGIPWYEKPVVLAIVVGAISIVLTIILW"
     gene            33015..33155
                     /locus_tag="PKI35_04855"
     CDS             33015..33155
                     /locus_tag="PKI35_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43747.1"
                     /translation="MVIRNQQVRLYRRRFRKIAGEAGGGPMACRLTHNLAQAGKLLVV
                     SY"
     gene            complement(33833..34855)
                     /locus_tag="PKI35_04860"
     CDS             complement(33833..34855)
                     /locus_tag="PKI35_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNX43748.1"
                     /translation="MKQVAIGIDIGGTNTAIGVVDRDGNVLYEKKPQLPTPQKNQNPL
                     ADVARSEELLSEYIKSLTGEIRSAIDTVRQINPDLEVLGIGIGAPNGNYYSGTIEYAP
                     NLPFVGVVNFAKRIEEKFPDIKYVKLTNDANAAALGEMIYGGGKGMKNFVMITLGTGL
                     GSGLIVNGDLVYGADGFAGECGHTTIVPGGRVCGCGGAGHLEAYCSATGIKRTVYELL
                     AKYNNSESELARVPFNDMTSKIVFDAAEKGDPIAREVFEATGELLGRGLADTVHHLSP
                     EAIFLFGGAAAAGDYIFKPTRESMERHLLPVFRNKVKILPSELKAGTSAIVGASALVW
                     KEMEKG"
     gene            complement(34883..36289)
                     /locus_tag="PKI35_04865"
     CDS             complement(34883..36289)
                     /locus_tag="PKI35_04865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX43749.1"
                     /translation="MEQKNNSNLKLGMSFMAAIFFIFGFLTNFNIAMKDQVQLAFDLK
                     TLYPGWENFLAQLVNGVFFFAYACFSFLCGGIIKKIGYKSGVITGLVLAGLGSYLFFP
                     AVEIVSYPLFLVAVFVMATGVVFLQTAANPYVAALGSQETSAARLNLTQALNSLATTI
                     APFLAGILVLSPAVMAISNGSTPAEAAKFVQMPFIVIGSIVLLVGIGLAFVKLPVITG
                     DKTIPSKSVLKKPQVWLGAIGIFCYVGAEVGTAGQIPPYLKESGFGIDVAVKLSAIYW
                     GGAMIGRFFGSILLSKIQNARKYQYSALVMVFAFFVGWFITSSSVTDGKFVFESQPLN
                     GLIFFAIAVINFFLMLLGKGKANVALGIFGTVNMILIVAAYLLPADLGMWCLLSIGFF
                     NSIMFPNIFSLGVSDLDPSEMPIAAGIINTLIVGGAVVPLLMGAATDSFSARGALIIP
                     IICYAYITFFALKGSKIR"
     gene            36651..37778
                     /locus_tag="PKI35_04870"
     CDS             36651..37778
                     /locus_tag="PKI35_04870"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HNX43750.1"
                     /translation="MKKASKITGLAMVSVLIVTLAAGCCKKIQTMNITKEAFGMLADS
                     VPVSLYTLTNDKGITLKVTNYGGIITSLLVPDKDGNEGDIVLGYDSLSGYLTKTPYFG
                     AIVGRYGNRIAKGVFKIDGNEFRLPVNDGPNHLHGGIRGFDKVVWDAKEFKTDSTVGL
                     VLHYLSKDGEQGYPGNLDVTVTYTLNNNNELRFDYLATTDKPTPVNLTQHSYFNLAGD
                     GDIKGHELLIKASKYTVVDSTLIPTGELRDVKGTPFDFTTAKTIGKDLAATGGNPTGF
                     DHNFVLDTKGLQEAAIKVTEPASGRVMEVFTDQPGVQFYSGNFLDGTIKGKGGKVYNQ
                     YNGFCLETQHFPDSPNQPAFPNTILRPGEKYQTTTIYRFSK"
     gene            complement(37840..39084)
                     /locus_tag="PKI35_04875"
     CDS             complement(37840..39084)
                     /locus_tag="PKI35_04875"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNX43751.1"
                     /translation="MTCRLPAKVAISYQILSIFAGNNSKTTSMAIAALQKEFKSRYGI
                     GEAPALFFAPGRVNIIGEHTDYNGGYVLPCALSFGTYLAVRPNGERRMRFATFNFSYA
                     ADVPFGDLAKKQGSEWVNYPLGVIDQFVKKGVELQGFDMLFSGDIPNGAGLSSSASIE
                     LVTAVALDEMLDSGIDRIELIKMGRAAENQFVGMNCGIMDQFAVGMGKASSAIFLNCR
                     TLEYELVPVEMEGYSIVIMNTNKRRELADSKYNERVRECASALESLRSIRPLTDLSDM
                     EIAEFLENEKVIADKTIRNRARHVISENRRVLDAVAAMKRGDLTTLGKLMNASHESLR
                     TDYEVTGKELDTIVHEAWKVEGVVGARMTGAGFGGCAVAIVDDDSIDELIACVGPVYH
                     ATTGLSADFYVASVGNGAGRIG"
     gene            39091..39945
                     /locus_tag="PKI35_04880"
     CDS             39091..39945
                     /locus_tag="PKI35_04880"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNX43752.1"
                     /translation="MPVSKKGTFMEIRGKYFILNGKQEPAEVFQQLSAIEGPTLYEVF
                     RIINGIPLFVEDHILRFENSAGLAGLVLPYTRDDIFRHVTILIRANRFRNANIKLIAC
                     YPSGKPPCYAAYFVEHQYPKPSEFRLGVDTITLRAVRQNPNAKIFHTALREETDALKK
                     HLKVYEVLLVDNNGNITEGSRSNFFCIRGNQVITPPVHEVLPGVTRKYVLQACHNLKC
                     PVSETSVPAGDLPSMEALFLTGTSRKILPIRKVDGGSFSVSHPMMRALMEEFGRIVDD
                     YLSRHDKL"
BASE COUNT        10941 a         9816 c         9139 g        10220 t
ORIGIN      
        1 tgaatcatga gaaagtaagc cttcagggtt tcagcgccgg catttattat tacgtcatca
       61 ttgccggcca ggaaaggcaa agcgggaaac tgatcaaaga ataaggggcc gcgcccagct
      121 tttaatactg caaacaggca attacccggg acgccgatgg gtaccggatg ttttaccaac
      181 gaaatccatt tatgaagccc tggtaattgc ctgattgttt tatcaatatt gtcgtccggc
      241 agaaaattgg ttgctcactt tgccgccaca ctccggattt ttacagcatc acgggctttt
      301 caaactttcc agccaatggc ctgtacaaat aacctattca agcttatcat tttaatatat
      361 tcgctcaacc aataaaggcg gctcaagatg aaaagatgct tctctcctct cctgtttctg
      421 atgatcggct atatcgtaac cggttgcaac acatccgccc ttcaacgggg cgataacaga
      481 gtgcggccgc cggccgatac ggtcggtttt gcgcatcttg accggcagat cgattcggtg
      541 atgaaccgca tcaaccggaa ctattccaaa gaattgccgg aagtaatcat ccatacggat
      601 tcctgctgga gggttgccat ttgtccccac gacgattatg cctatgcaag ctggcaatat
      661 ccggccatac tccggaacat caaggctaaa acggtcctca tttttggcgt tgcccataaa
      721 gcgaaacaat tcaatatttc cgaccgcatc gttttcgaca gctatcccta ctggcacggc
      781 ccttataaaa acatcaggat atccccctac agggaagaga taatggcttc aatgccccgt
      841 caatattacc tggtcagcga cagccttcag gcggcggaac attccgttga aggcatgctg
      901 ccgtttcttc agtatttcaa ccgggatata gagatcattc ccatccttgt tccttatatg
      961 gatgcggcca ctacggaagg aatctcccga tcattgtcca cggctgtgtg gcgtgtgatg
     1021 aagaagcatc atctgagatg gggaaacgat cttgcgctcc tgatcaccac cgatgccgtg
     1081 cattatggcg atgaggactg gggcggaaag aacatggctc cttttggcac cgacagcgcc
     1141 ggttataaaa aggcggtcgc ccgcgagcat gccatcatcg acagttgcct tacaggagaa
     1201 ctcaataagg aaaggatcgc gcgattcatt cattacacgg ttcagccggg cgattataaa
     1261 gaatacaaat ggacatggtg cggcaggtat agtattcttc tcggactcat gaccgccatt
     1321 aacctccgtg aggaactaaa cagcgccccc ctttccggaa aatgccctgc ctataacacc
     1381 agcatcgatc acgcgccgtt gcccgtggaa gacctgggga tgggccggac ggctatcgcc
     1441 agcctgaggc attgggtagg ttatgtaagc gtcgggtata aatagagcag cgtatccccg
     1501 gcaacaggcc cttccggttt tcagtctatt cattatcaga ataggaatcc ggttcttttt
     1561 tcttgttgac cgacaatacc ttaaaaaaga aataaccgca taaacctgtc acgattgcac
     1621 tcaccgacag ccagagaatt attgcagagg tgttcataag cccagcattt ggtgttgaag
     1681 tatgtaaacc atggcaccgg caaagtagcc gagcaaggca aacaggctga ttttccggag
     1741 ataccagaag aaatttattt tttccattcc catggctgcc acgccggctg ccgatccgat
     1801 aatcagcgcg ctgccgccgg ttccggcgca ataagccagg aattcccaga agtaatgatc
     1861 ggtggggtac acctgcaggt catacatccc gatggctgcc gctaccagcg gtacattatc
     1921 caccaccgac gaaagaagtc cgatggaaag gacgaccagg tttatattcc tgattttttc
     1981 ttccataagc acggccagat gcgataacat acccgaggac tgtaatgccg aaatggcaat
     2041 gagtatcccc agaaagaaca acacgctggg catatcaatt cggtgcaaag catggcgaac
     2101 agagtacctg cttttctctt cctcgttttt atctttatgg acgaattcgg aaagcagcca
     2161 caatactccc agcccaaaca tcattcccat gaacggcggc aggtgggtaa tggtcttgaa
     2221 aacgggaaca aagagcagcg caccgacgcc tgcaaagaaa aacaggttac gctcaaaagt
     2281 cgaagccatt ggattgccgg atgcattaat gaccggcaat ttccgcgtat ttccttttaa
     2341 catgaaggta aaagcgatca gaggcacgaa aagggaaacc aggctgggca ggattaattt
     2401 cacaattatc ttccaggtag aaacctgtcc gccgatccac aacattgtgg tagtcacatc
     2461 cccgataggc gaccatgcgc cgcccgaatt tgccgctatg atcaccagac cggtgaagag
     2521 cattctttcg tttttatcgg agatcagttt cctgagcagc gtaaccataa cgatggtagt
     2581 ggtaaggttg tcgagtaccg ccgaaaggaa gaacgtaagc aatccgatga tccataaaag
     2641 tttacgcttg ctggtggttt ttatcatccg ggtgatcacc tcaaatccgt cgtgggcatc
     2701 aatcacctca acaatggtca tggcgcccat caggaaaaac aggatacccg aaatttctcc
     2761 cagatgttcc aggagttgct cattcacaag atgcgaatct gcggacatca atacatatac
     2821 cgcccagcag attacgccgg tcagaagggc gcttgccgct ttgttaaggt ccagcttatg
     2881 ttcgagaata ataaagatat agcccaggac gaagataagg gcaatgatta tttccatctg
     2941 caagggggta aaggtgagtt aatggtaaag tttattcgct gagcgccacc gttgagctta
     3001 tccaaccctc atcgttctcc ttctctatgt acccgggttt tattaacctt ccgcggtcaa
     3061 tcctcctgat gatcgctcct gcctgtaatt gccggcttat tttacaagta atactttgat
     3121 aagtatcatg gctgcgataa ctgcaacaaa aacaagtccc atcatgagtt ctaccggaat
     3181 tttacctttt ttcattttca attttaggtt taatatttaa tgacaaacct aggcaagaat
     3241 gtccaaccag ataattaaaa tttaattaga aaatctcagg aaaaacagca tatatctttg
     3301 gcgccttcgg tgtttccaat atccgggaat ctaaataatg atgcagtaaa ttgatcttgt
     3361 atacatagta ccgtgcccat ataaacattg ggagctatta gttatctact cttttttctc
     3421 aatttccttt gttggcctgc ccgacagcat taggccaaac atcatgacca gcactgaaag
     3481 aacaatcgtt tgaatgacca gtgatttacc aactaccggc attctttgat ccggcaggat
     3541 ggaaataaac aacaatattg taatcaggcc acgcggggca acaaacaaca gttccgtggc
     3601 aggcagttta aatactttta atgtaaccca gcgtatcaga taaattgata ttacaatacc
     3661 gcaagaccat ggcaatgatc caagatcaag caattcatca gtttccatca gatagccaaa
     3721 gaggaggaaa aataaggacc tgacaagaaa ggtcacttca taggttatgt ctttgaattt
     3781 caccacttcc gcttcaagtt tctgaggcct gaattgttca aatattttga accttttaaa
     3841 ttcttctgta ttcccgagaa acagtccaaa tacaagaata aatataagac caggcaaatg
     3901 aaactgtttt gaaacagcat aaataagaat aacgagcagg attataggag tgtatgtaat
     3961 atgatgactt atccggctca ataaaaaaga aagcatcaat actaagataa aagaaccaat
     4021 aacgataact atcaactggc tgccaaaccg aaagaatata tgcccgttaa atgtattatt
     4081 aatagcaata aaattaaaga gcaacactcc aataatatca gaaaagctgc tctcatatat
     4141 taccagttct ttctttatcg ggctaagatt tcgcacactt ggaattgcta ttgcactgct
     4201 gatcacacaa aacggcaaag cattaagcaa ggctgtttta aactcaacaa cctgaaaaaa
     4261 ataaaatact ccggcaatta caaataacag caatagcatc ggaatcaagg caagcaacaa
     4321 ggctttgcct ataacgccga ccttcgccat atttatttcc agttccaggg caccttcaag
     4381 aacaattaat atgagcccga tcgttcccag aaccggcaga accggattaa ggtcaggtat
     4441 tgaaattttc agcattatag ttgtttgccg aacaatccat cccaggaata ataggaggat
     4501 aaccgaagga atccgggtac gcgctgaact tatatcaaat aaataagaca gtaacagaag
     4561 aaggcaaata aagacaatga cctggatatc catataaatc aacttatcaa ccagaacaca
     4621 acactaaaga tagactaatt tatcattgtc gataagagaa aacgacctct aaattttaac
     4681 atgaaaacca tcgagaacaa aacttacacc ttttgcgcag ataaaaatat agagtattcg
     4741 ggctattacc attacgccga ttttcccgat gattttttca acccagggag ccaaatacag
     4801 caaaggcaaa atggcagcat aaacgagcaa tattgtcgaa acggttataa tgtaaccatt
     4861 ggcctgtaag agcaagatgg tggtaagaat tgtcccggga ccggcaagaa aagggaaacc
     4921 cattggaaat actgcaacat cactgcttgg ccgattctga acttcacctg atacctgacc
     4981 gcttaaaacg cctttggccg caactgcaaa caccagtagt cctcctgcaa tcttaaacgc
     5041 tgatgtgctg actacaaata cattccgcaa cataaaatcg ccgaacaggg caaatatgag
     5101 cacgatgctt cctccggtta acacggctac aagaaacgat ctgctctttt cctttttctc
     5161 catttgagct gtcagatcgg caaataatgg aagattgccg attgcgttca ccatggcaaa
     5221 gaggataaga aaattatgaa tcaagaggtt gatagtgctc atttctcttt cataacagga
     5281 cttcctttcg ttctgcccat ccattccagg ttttccagat cgccgaaatg ttcccggaat
     5341 atgcggtagt agaaaagttc ttccctggaa tttaacatcc agccgttacg cagtttacgc
     5401 tcgttctcaa aatcgcgccc tgaaattttc cggttggcat aatccgatat aagggattcg
     5461 accccagcac cttcccaaaa ttttgcttta ggcctggata agatactatc gggtaataat
     5521 ccggacatag cctttctaaa tatccatttt tcaactcccc cggtaatttt atacccggac
     5581 ggaatacgaa gggcaaattc aactacttca ggatcgagaa ggactacatg cgccgacagc
     5641 ccgaaagcag aagcggaacg atccacacgt tgcagggctg tattatgcaa ccgtttggta
     5701 atatcgatca actcgctgtc gagatcggcg ggggggatat ctttcagata gctgtatccg
     5761 gcaaacaact cgtcgccgcc ttcacctgaa aaaacatcag taacatagtt ggaagcggcg
     5821 tatgcaacac aaaggtttat tatgctggaa cgtaccagga gtgcgtcgaa cgattcaagg
     5881 tgatagatta catcgggaag aattttcagc atcatttccc tgtcaataac tacttcatga
     5941 tgctccgatc cgataaatga ggctgcttcc cttgcaaaac gaaggtctgg cgcttctttc
     6001 acgccgccag caaaagtgta aagtttattt acattcggtt tagccaaagc agctatggca
     6061 ctcgaatcca gccctccgga gagccaggct cccgctgtgc cggagtttat ccttcgggcc
     6121 acaactttct gaagcagttc tttcagttgt tcagcaatct caagcggacc gtcagtgaga
     6181 ggttcccctc tcttcagttc atgataggta acaattttgt tgtttgaaat tgctttaccg
     6241 ggcaacaatt caacaatatt atcagttaca ggcaatatcg ccttgacttc tgaagcaaag
     6301 acagtctcat tatctattgt acccaaatat aaaggcacta ctcccaccct tcctctctca
     6361 atcctgacat ttccgtcatc ggcggtaacc attacatggt gcccgtatcc gggtccaatc
     6421 ctgaaaatat tctgatcaaa aagattacga acatggtcat tttcatgcag cgaccatatc
     6481 attcctaccg tcacttcgtt ggtttcaata atttccttcc catattcgcc cctatgcgat
     6541 atgattccaa gcattttttc gacaagttct ttttgtccgg atttcaatac gcctgcaatt
     6601 ccagccatag tattcaagtt ttaatttata attgggttaa agcctttgta gtattcctga
     6661 cagtctcgct ttctcatcaa ccggttctcc cgaaaagaaa tctatcgggt accatgctcc
     6721 atcaataacc cgcgtcagga tatttccgca taagtgcggg tttcctttga aatgaggcgc
     6781 gtcaagaatt cccaccctga cagcttttgc aataatctcg ggatcggacc aggcatcttt
     6841 gcttttgccg agttttggcg atttcaaagc ttcaaggatc acacgcgctt catccttaag
     6901 ctcatttttc cgccttaaaa ccgaactatc aattgtcaga tcagggaagc catgcaatac
     6961 atttctgagc acgccatgaa cgatacggca actctcgatc agttcatccg ggaaagttgc
     7021 atgatcgcct tcgctgtatc cgacaacatg caggatgtga ggtttcagcg acaggctggt
     7081 gacggccgac gcagccagtt gtccttttgc cactgctggc accggtgaaa aatgggcaat
     7141 tccggcacgt acttccctga tcatctcgaa attttcatcc tccagttcac tgatcatttc
     7201 cttcttagcc agcatcttcg ctatatccat agcagcggag gttccgggcg gagtattaaa
     7261 cataaactgg gatacgtatt ttttcacacc tgcctttttt gcattatagg cagccagaaa
     7321 tgccatggta acggcaagtg aatcatgagc atccctcagg ctccactgat gggattcgtt
     7381 cacttccacg ggaattcctc tttcagcata ccatcgcatg acagattgat tctcggctat
     7441 agcctcttcg agcgtacgtt gcgaacggcc gtcaattacg ctgtaccaac acaggggtat
     7501 ggccccccat gcattgtgaa tcgtcctgac actcatttcc gcccatttca tcaaatccat
     7561 agttccagca taacatcgca cgagcggaaa attcccggtt cgtgaggcat tgtaaattgc
     7621 ttcaagatct tcaggtttgc gaagtggcac acctccggca ccatccatct catgattcat
     7681 cagcccgggt tgaaaaaaat gttcctgcgc attctggtca gggccaattg aaagaacatc
     7741 caaaacaccg gcttcggcta tcgtctgagc gcctgttacc gtttcttcaa gggtcggtct
     7801 gccaaaatga tgcctgatca gcgggtaagg atatttttgc gcaatcctgt caatcaggtt
     7861 atcagcataa atagttccgg caacttttcc ctgacctccc tgtaaataga ttcttatttg
     7921 ttcaaccgat tcagtcccat caaaagagtg ttcgaagatt cccgacttac gcgctacttc
     7981 tgcaacagga aaagggccgc cgaaaatcag ctttatatca ggaatagcag ctttattaag
     8041 ttcctcttta agttgcgaaa acaatccttc ggcaacctca ggcgtcaggc gataacttac
     8101 ggcgactata tcgggatttt tctcccgtaa agccctgatc agccgttcaa tagaaacggc
     8161 agggcccagc gataaggttt catatccttc cgattccgca attttcagga aatgatgtaa
     8221 tcccgccaca tgaacgcagt ttcccaaagc cgctcccatg atcagttttg ttttcataat
     8281 ccggattcct ttctcaaata tgtgatttta ccggttctcc gatggccata agccggatat
     8341 cccgcagggt cataccttcg attcccattc tccggacagt tcggcctata gcccaataat
     8401 ccactccatt caacagggat gctatatgaa tcagcgaatc aatggtcggt gtgggcacct
     8461 tgaacatcct gccgacactg gcaataggca caaggctgca cggaacatct tcctcaaggt
     8521 atctcatttt gagggtcctg ggcgccatga tccctctgta accgggattt tttcgcatcg
     8581 cttcgaagag tgtgttaccg gcagtactat aagccatgta aaaccattgc cgtgccgaaa
     8641 tcgcctggat accaagtgcc tcagccaccg caatacgttc ggcgtctgtt ttttcaagaa
     8701 ttttaaccac cgactcggtt gccccttcat gataaaactg gaactctgca tcatcttcaa
     8761 tccagccgga gttcaatata cacagggcag gatggaagac actcccgata ttttcgaaac
     8821 ttgttttaaa aatattgtca ccgggaacaa actgaggata aaacacgcgt aactgactga
     8881 taacaacagg tataagatga gccctgaccg atgccaccgg aatagaatgc ttaatcccga
     8941 agattttcac ctgtgatggt cctatagccc gggatgcata aatgaaggtt tgcgcctccg
     9001 atacaataac atcggctttc actttcaatg aattcaatac ttgtctgaat tcgagcgccc
     9061 caagggtacg gccgggatgc agtattatca tctgcccgtc tttcaggcag ggagcgacgt
     9121 tttccgccat ataccggtgc gcaacggcgg gtactactac catgataaca ttggcattcg
     9181 aaattgcctc ttccatattg gtggttatga cttttaaagg gccgaacccg cttacttcgc
     9241 cagtcaactc aataccgcct gatgatttta caccccaaag acgttcctca ccacggttga
     9301 aaaggttaac ctcgtaaccc attaacgcca ggtgcccagc catagccaat cctccgtgcc
     9361 cggctcccag aaccgctatc acaggctttt ccaacacttc aaaaagcttt ttcatgacca
     9421 gttttcttta cattcacccg caaatgcggg tcgttttttt taaaaaaaga ttgccttcac
     9481 tgctatcaat attcctatta ccaatgccca gaacaggaat attaataatt ccacaggaat
     9541 ttttttgttt ttcatgattt gtgagtttaa tttttagcca tttactatcc gcgtacatgt
     9601 tatcgttttc aggtaaaccg attctataaa ctccgccttg ccggactatc tcttataaat
     9661 tttagcctct cttcagagaa ttgataacct gacgtctcag tattcgacat ggaggataag
     9721 agaatccagg tctatcctga aaggtatctc tttttcacac aggttgattt ccgacataag
     9781 attcccgcat actttgcttt tctctttttc attaaggtag aatccttcta catcaaactc
     9841 ggggacgatc tcatggaatg tcccttcatg atcaattatg caaaggctcg tttccgttcc
     9901 tgcaatacag cattcgggac ggcccgattt aaccgcactc agaattgtct ttccatcgtc
     9961 ggggaacctc tttatttctt tcttgtcaat attcacaaaa tatcccttcc cgcctgctac
    10021 gacaaggctg aaaggagaat cactgcaagt cagcactttg cataaaccct ggttatggcc
    10081 ttttacaaac tgccctatcc atgactgtcg tgaattgccg gtaaacatta ccagcatctt
    10141 ttcgctacct ccccccgcat actctacttc atcgaatttt ctcaaacacg gcttatgccg
    10201 tattattttt gctttcatgc catcattatc atttagttca ttgatttact tattgtcggt
    10261 tttaaagata gtcccgaacc gttgaaaagg cagtaaattc tctattagaa aaagattaga
    10321 aattgattag aagtaaaatc cttggcttct tgtcctaatt ttgcagtaaa tgattttgtt
    10381 atgtcaaaag ccaggatttt agttgtagag gatgaattac ggctcgccaa agtacttaaa
    10441 aaacagattg aggaagccgg attttccgtt cagatagcga atgatggtta caccgggaaa
    10501 aggtatatcg aagcgaacaa gtacgacctg gccatcctgg atattaacat tcctattatc
    10561 aatggttatg atctctgtaa agaaataagg aggattgatt tctctattcc catcattatg
    10621 cttacagcat taggaaccct cgacaataaa atttctggtt ttgatgccgg agccgatgat
    10681 tatgtaacca aaccatacga aatcagggag ctgctggcac gtatcaatgt attcctgaaa
    10741 agatcggatg tcgatccctc ttctcacatc ctgagcgtag ccgaccttcg gatggacctg
    10801 gtaaataaaa ccgttacccg ggctggtgaa aaaatcgatc tcaccgtcaa agaattctcc
    10861 cttcttgagt tattcatgaa gaacaaagga aagctgttgt cgcgtgagtt tattatcaat
    10921 aaggtatggg gcattgactt cgatcccgga accaacgtga ttgatgttta tgtgaattac
    10981 ctccgaaaaa aaatagacaa ggattttgat ctcaaactta tccataccaa atttggattc
    11041 gggttttatc tcaacgaatc ggaaatctga ttacttatga atatcaaggt acgactctct
    11101 tttcaattta tgctgatagc agcaacagtc ctagtattct ttgctttact ggtctattat
    11161 ttcacattta ccactaaccg ggaaaaattc cggaacaacc tgctaaacag ggccaagaac
    11221 actgccatca tgctgattga tgttaaagaa gtggattcta cgcttctgaa aaaaattcat
    11281 cagtcaacta tttcatggca gagcgaagaa atcgcagtca ccgatacgaa ccttcacatt
    11341 atttacagca ataacattaa ctatttgtca aataatgttt tccgctcaaa tatcagcagg
    11401 gataatcatg cctattttac tttatcgggt aaagacggag ttttttacag gcatgtatat
    11461 aaaaataaga ccgtttatac ttttgtatta gcatacgaca aagcccgagc cgtgtttctg
    11521 aaggaactca ttcagatcct tttctggagc atccttttca gcctctggct ttctgttctt
    11581 ttttcctata ttttttcgaa aaaagccatc aaaccaattt cggatatcat tgattccgta
    11641 aaagccataa attcatcgaa ccttggcaaa cggcttcatg agggaaaccg aaaagatgaa
    11701 attgagcaac tggccatgac gttcaacgaa atgctgttta acctggaaat cgccttcaaa
    11761 aaccagcagg aattcgtttc gaatgcctct catgaactaa aaacacccgt ttcggtaatg
    11821 atagccgaat ctgattattt tctcaataac aaacattcgg atgaagaata caggactcat
    11881 ctttccgaaa tgataattga ccttaagaaa tttaatacat atataaattc cttgttggat
    11941 ttagctcaga caaatgcagg taaaagtatc ctgtttaaag aagaaagact tgatgaaatc
    12001 ctttatgacg ctgtgcatga aatcaaaaac agatatcata accgtaaaat tgtaactaaa
    12061 atccattttc ccgaaaacga caacggcctt agcattgaag gacatcctgg cttattggtt
    12121 attgcaatta aaaacattat cgataatgcc tgtaaatttt cagatgaagt tgtaaacgtt
    12181 gaccttaagc cagccgacgg aaccataaag ttaactattt cagattcagg aatcggcata
    12241 ccactgaatg aaattaatga tgttttcaaa ccatttaaca gggcatcaaa tgcacgattt
    12301 aaaagcggtt tcggcatcgg gctgtcgctg gttttcaaaa taatggagat acaccatgtt
    12361 gaaatggagg ttttcagtga agagaataaa ggcacccggt ttgaattgac cttcaaacgt
    12421 tcagcaacag aatgaaccat cattgcgtcg ctctactcct ctttatccgg gctggccgcg
    12481 actgagcctt taatcaactg caaaacaaac cgttttgcga gtcgtaactg tttcatttga
    12541 aagttttttt ttgtaattta actctccaat caagaaggca tgaaccccta ttcctggttc
    12601 cggattgtat actttacgct ggttccgatt gcctttggca attgcaaagg ccaaaaccgg
    12661 acggaagaag actatacgcg gctcaggaaa gaaatggtgg aacaccagct caaaagaagc
    12721 ggaattcatg atgcgaaggt cctggaagct ttcctgaaag taccgcgcca catctttgtg
    12781 ccggtggagt acagggatca gtcctatgat gacactcccc tgccgatcgg ttacaaccag
    12841 accatttcgc agccttttat cgcagccttc atgacggaag tcctgaaacc cgataaaagc
    12901 atgaaagtcc ttgaaatagg caccggatcg ggatatcagg ccgccattct cgcccttttg
    12961 tgtaaggaag tatattccgt tgagatcatc gattcactga ctgttagggc ccgaaaaatc
    13021 ctcgaaaaag aaggctttaa gaatatcaag ataaagacag gcgatggtta cctgggctgg
    13081 caggaatatg ctcctttcga tgccatcatc gtgacctgtg cgccggcgga tataccgaaa
    13141 gctttggcgg atcagctcgc tgaaggcggc aggatgatta tccccacagg cggatcctat
    13201 aaccagaaac tctatctgat agaaaaaata aacgggcaaa tcaggcagaa ggagactctc
    13261 cgggttctgt ttgtacctat gattcacggc gaaaaccgac aataataaga gggtttgcac
    13321 cgaaccggac ttccattttc ttacggtcat ctctttggat ttcagctctc ttcaggcttt
    13381 aacaggaaaa cacaatctgt cttcttcctt tctttctgtg aagtttcgaa gtaaaattct
    13441 tttcttcttc aagtgaagcg acgctattct ggcatacaat gattaatttt atcaactcaa
    13501 aaccgcagta tatgaaacgt cgagatttta ttgccggtgc ggcagcagcc ggattgatcg
    13561 gaaccggccg gtttgccttc ggaaatattg agcagtttgc gccgtcagcg cccgttttac
    13621 cttacgatct ggttgcagtg cgtggtggcg aaccggaggt aatgttcgac aaggcaatac
    13681 aatccctggg cgggatgaaa gctttcgtta agaaaggaca gaaagtcgtc gtcaagccca
    13741 atatcggctg ggactcatca cccgaaaggg ccgccgacac caatccgaac ctggtgaaac
    13801 gcatcgttaa agcatgttat gaagcgggag caaaacaggt ttatgtattc gacaatacct
    13861 gcgataactg gacaaactgt tacaagaaca gcggcatcga aaaagcggtg aaggatgcag
    13921 gcgggacaat ggtaggcggc aacagtaaaa gttactacca ccaggtaaac atacccaggg
    13981 gcaaacggct gaaacaagct gaagtccacg agttgatcct tgattcggat gttttcatca
    14041 atgtgccgat actaaaacac cattcctcgt ccgatctgac cattgccatg aaaaacctta
    14101 tgggtgttgt gtgggaccgg gggtactggc atcggaacga cctgaatcag tgcattgctg
    14161 attttgccac ctggcgcaag ccaaccctga acatcgtaga tgcctaccgc gtgatgctgc
    14221 gcaacgggcc gaaaggcgtc tcggcatccg atgtcatcac catgaaacag cttctgattt
    14281 caaccgacat cgtcgccatc gacgctgctg caacaaaaat atgggatccc aaaaaggatc
    14341 cggccaaagt aaaacatatt gccctggcca atgaactaaa ggccggaaat atgaacctgg
    14401 cccaactcag gattaaccgt attaaactgt aacatgcatt cacgggcact tcgctacata
    14461 agatacattt ttgctgtatt cttcttcctt ctcatttcgg ctttgtttct ggatttccgc
    14521 ggcctcgttc cggcggatat gcacagggct gccacctggc ttcaattcgg cccgtcattg
    14581 gtgcagttca tacattttgc cggattgttt tctgcaggat ttctttttat cgccatcctg
    14641 accttactgt tcggaagggt atactgctcg gcaatttgtc ccctgggcat ttttcaggat
    14701 atcctgatca ggattgaaaa gtggtttcgc aaaaaaaaga tactccgtta ccggaaacca
    14761 ggctggtgga gatatgtatt cctgggcatg gcggttctat ccctgctcgt tggttttatg
    14821 gcccttttta acctgcttga tcctttcagt aatttcggaa gaattttctc ttcgctgggc
    14881 aaaccactgg ccatcctgat caacaatgca gcagcagcga tatccgaaaa atcaggatta
    14941 gccgtcatca accgttatcc cattaaagga tttgatgtcg cgaccctcat ttttcctttc
    15001 ctggttattt ccctgctcat tttcctcacc tggaagcacg ggcgcctgtt ctgcaatttc
    15061 atctgtcccg tcggaaccgt tctcgggctc ttttccaggt tttccctttt cagcatctcg
    15121 ctggataaaa caaaatgcaa ccaatgcgga aaatgtgccg ccaggtgcaa agcaggatgc
    15181 atcagtataa aaacacagga aattgaccac tcgcgttgcg tgatgtgttt tgattgcatc
    15241 gggccctgtc ctgaagaggg ggttactttc ggcctgtcat cctttaaaag gtcatctcag
    15301 gttgcaaccg gtaaaaaggc ttccgactca aggagaaaat tcatcagttc agctttcctg
    15361 ttaggattgg caggccgttt ggcagcccgg gaaaacagcg gcgacacatt tgaaaggcaa
    15421 acgctcatcc ctgaaaaaaa ggcatctccc gtttcaccgc cgggttcacg ctctatcgaa
    15481 caatttattt ccacctgtat cgcatgccat gagtgcgtaa gcgcatgtcc cgcgcaggta
    15541 ttgcagcctt cactgttgca atatggctta cagggattta tgcaaccgca catggattac
    15601 catgccagct tctgcaattt tgaatgcgtg gtctgcagcg aagtatgccc tacgaccgct
    15661 attcagccga taacggtaga agagaaaaag aaaatacaac tcgggaaagc cgtctttatc
    15721 aaagataatt gcgttgtttc taccttcaat actgcctgcg gcgcctgttc ggaacattgt
    15781 ccgaccaagg ccgtcaacat ggttccttac ttggatggac tccggattcc ggaagtaaac
    15841 cagtcaattt gcataggctg cggcgcctgc gaacacgcat gccccactaa accttacaag
    15901 gctatcttcg ttgagggaaa cccggtacat cttactgccg ctaccccgaa agaagatata
    15961 atcgaaagaa acgtcgaaga ggaagaatgg gcattttagg ggcagggaga acggagaacg
    16021 gaaaacggag gacggagcaa ggagcaggtg ccaacggcca acagctaaca gccaaaagcc
    16081 accagccaaa agctaacagt ttacctgcct gggctgattg tgcgtaagac ggcaggctaa
    16141 cggctaacgg ctaacagcta acggcccaaa attccccgat caaaataaga caacaattat
    16201 taatttagca ctttcaaaac cgatcaccat gaacacagcc ataatccctc aatatcgttt
    16261 cttcagcatt cggtcgctgg taagtgacgc aatcgcgctg gcttttatct gcctggttcc
    16321 cgccatttca catttgtcgg gagtaccggt ttattttatt gagccaatgc ggctgatgct
    16381 gatccttgct atattgtcat cacacaaggc caatgcttac gcgctcgcaa tcatcctgcc
    16441 ccttgtttca tttctggttt cgggccatcc cgaaccgctg aaaatggccg tcatcatggc
    16501 cgaactactg gttaatacct ggttgtttta cctgcttttg gcccgcacgg caaagccctt
    16561 tatcagcatg ctgacatcca tcgtcgtcag taagatattc tgctatacct tgtacctggt
    16621 tgtgttttcg ctggctttcg taaaggcaga agcaggaacc gttttcctgg tgtcacagtt
    16681 acttgtcacc ttgctgctga gtgtgattac ttatcttttc ctcagcgcaa aacaacaaaa
    16741 atcgaaaaat taaccgcagc ttcctttttt ctgcagtcct tcctttcctg tcattccctg
    16801 acggtaaaac cgcttcagaa ctttggtttt tgtcgataaa aagcgcctgt ctgtctgcat
    16861 ccttttcagc aacttataaa tagttttcaa gcgaaggtat ccggatatat gtttgcagcc
    16921 tcaaccggat ggggcaagga acctatgatg ggacaatccc gggaatattt tttttaatca
    16981 atttgtttca gaatgaagaa cagatcatca ggtattacat taatactcat acttacggca
    17041 acgatttcat ttgcccagag ttatgaagcc agatggcaga aaacgccggt tatggccgac
    17101 ggaatttcat cggaatggag ctctccgttg cgtttcagcg atacaaaaag cggccttcag
    17161 tacgctgtaa cgaatgacga taaaaacctg tatatctgcg taaaagcgac gggcactgcc
    17221 acccggatgc agattctcac atctggtatg aagatttgga tcgatgcaaa cggccggaaa
    17281 aaatatccga cttcgattta tttcccgatg ccattgtcgc gaggtgaaat gaagcctgat
    17341 aattcagggc tgcagcaagg caggccgggt ggtgaaaggc catctgggaa acctgatgag
    17401 atggatttcc ggaaaatgtt caaaaatcag gggctaaaaa tcacccttgc cggcttcaaa
    17461 accggttgtg acggaacttt caacatcggg gaaagcacac aaataaaagc tgcgatggac
    17521 cgggacgaaa gcaacgccct gacctgcgag ttcatcgtcc cgatcggctc tttttacagc
    17581 aacgactgga attctttcaa ggaaccgcct cttatcggaa tgaagatcag tgttgatgaa
    17641 atgcctggca acgggcaagg aacacagcgg cccgaaggcg gccctccggg cggcggcccc
    17701 ggtggcttcc ccaatgacat ggacggcgga ccgggaggac ctccggatgg cgaaatgggc
    17761 gatcgtcctt ctcccccgtc aggcataacg ggaacatcac aggaagaaat ttccattaag
    17821 tttaaaatca ggctcagcaa gccggaataa ttaactcaaa tcacaattat tggttcagga
    17881 atgccctgcg gtaaaacggc agggcatttt atttttcccg catccatgct ggtattttta
    17941 catttcaacc tcaaaaaaca acccgatgaa gcaattaacc cggcactgat tttccggttt
    18001 tcattcgtct ataaattcgg gcaatttatc tataaacctt tcatcaacct atatatgttt
    18061 tttctttaag aacgggccag gataattttg ctcccggaaa ttaatctaca aggaaatgaa
    18121 actgagaatc agcaaactat ttattcttct catcagctta tcagcattac aggctgaagc
    18181 tttctgccag gaaaagcccg ccgtctggaa tttgcgatcc tgcattaatt atgccctcgc
    18241 aaacaacatt caggtcaaaa aaatgcagct tggcgtaaaa agcagcgaag ccgactatga
    18301 ccaggcaaaa gcaagcctgt ggcccggatt gaatgccggt atcagccaat ccttttcaaa
    18361 taaaagcagt atcaacggtt ccggtgaaag cgcttccctg tcagggggat attcgttgca
    18421 gtcggatgtt acgctcttcg acggttataa gctcaggaat accatccggc aacaggagat
    18481 gatggtgaaa tcaaaccaac tgagtgttgc agaatctgaa aattccatcc aactggcggt
    18541 aacatcggca tatcttcaga tcctatatgc ccgtgaatcg gtcattgatg ccgaaaacac
    18601 gctgaaatcg tccgaggcgc aactcgaaag ggcaaagatt ctttatgatg ccggctctat
    18661 tgcccggagc aatctggcgc aggtgcaatc gcaatacggt aatgataaat attcgctggt
    18721 ggttgcccgt aataacctga accagcagat tttgtcgctg aagcagttgc tggaacttga
    18781 catctcgcag gagatagaaa tttatttccc cgaaatcggg gatgaccagg tattgaaact
    18841 tatcccttcg aaagaagaag tttataacac cgccctcaaa aatctgccgg aggtggccaa
    18901 cagtcaactg aacgtcgata ttgcccggaa aaatgctgaa atcgccaaag ccggcctgta
    18961 tcccagcctt tcattaagcg cttcggccgg aaccggtaac agcaccgctt actccgatgg
    19021 ttttgtgacg caactgggaa acaattttta ccaaaatgcc gggttgagcc tcagcatacc
    19081 cattttcaat aaaaaacagg ccagatcagc tattgccaaa gctcagattg caactgaaac
    19141 ggctcaactc ggcctggctg aaaccgagaa aaatctgctc aaagaaattg aaaccgtgta
    19201 cctggatgcc gtttcggctc aaagccgatt ccaggctgcc aacgagcaga aagagtatgc
    19261 cctcaccagt tatacccttg tcgaagatca gtttaacctt ggtatgaaga acaccgtcga
    19321 cctgatcacg gctaaaacaa cctatctcca ggctcaacag gaatgcctcc aggcaaagta
    19381 tgctgccatc ctcaactata aactccttga cttttaccag caaaaagaaa tcgaattata
    19441 atacaaacac cttcagtaag atgaaaaaga aaagtttgat aatcacaggt atccttgttc
    19501 tgatagccgc tgcagcagca tggttccttt tcggaaaatc cgggaatcag caggtgatcc
    19561 gttatgaaac cgcgcctgta cagatcggca ctatcgcaaa caccgttacc gccaccggaa
    19621 ccgttgaacc ggtaaaacag gtggaagtcg gcacgcaggt ttcgggtgtc attaaaaaca
    19681 tttatgtgga cttcaattca caggtaaaga aaggacaact gcttgccgag ctcgataaaa
    19741 cccctttgct tgctgcattg gcatccacca aagcagaact tgcttcagca aacagccagc
    19801 ttgtttacca gcaggcaaat ttcaacagga tcaaatcgct ttttgataaa aaagccgtca
    19861 gtgaaaccga ttatgaaacg gcgctttatc aatacagcag cgctaaagcc agtgttgata
    19921 agatgaaata tgaagtggaa cgcgctcaga ccaacctcgg ttatgccatg atctactctc
    19981 ccatcgacgg ggtggtttta aaccgcgccg ttgacgaagg gcagactgta gccgccagtt
    20041 tcaacacccc tacccttttc accattgccc aggatttaac caagatgcag gttgtagcca
    20101 atgttgacga agccgatatc ggacaggtgg ccgaagggca aaaagtcagc tttactgtgg
    20161 atgctttccc gagtgacctt ttcgaaggta aaatcacgca ggttcgtctg gaacctacca
    20221 caaactcaaa tgtagttact tacagcgtcg tggtcgacgc ccccaatccc gaccttaagc
    20281 tgaagcccgg cctcactgcc agcatcacgg tcaccgtgaa ggaagccgcg gatgtgctca
    20341 cggtccctgc caaagcacta cgttttcaac cggattcatc gctactggca aataattccg
    20401 taaaaaccgt tgtaaatcag gccagcgctg ccaacctgcc ggcaaacaac ccgtcgcaag
    20461 cagaaaaaca aaaggacatg ccgtcagcgg ttaatgagga tgaaacgccg gggaccatct
    20521 gggtgaaaag cggcgggagt attcacccgg tgcaagttgt atcaggcatt tcggatgaaa
    20581 ccaatgttga gataaagagc ggcctgaaag aaggcgacct cgtggttgtg tcgatggtaa
    20641 agtccagcgc accggcagga aaggacacta ctgaacaaag ttcaagcccg tttatgccaa
    20701 aaaggccggg aagcgaaaga aagaaatagc tgacgcgtaa aaggcagagc gataagtaaa
    20761 acgcgagaaa atgcacagac aggataagac cgtaaactta gaattcagaa aatgaacgag
    20821 atcataaaag ctacaaacct gaaacgcgaa ttcctggtag gaattgagaa agtagcggca
    20881 ttaaaaggca tttcatttac gattcacggg ggggagttcg tcaccattat gggagccagc
    20941 ggatcgggca aatcaacatt gctgaatatt ttaggctgtc tcgatacgcc gacttcgggg
    21001 gaatatctcc tggacgggac ttcggtgcgg gccatgtcga aaaatgaccg ggcccatctg
    21061 aggaaccaca agattggctt tgtatttcaa acttacaacc tccttccgcg gactactgcc
    21121 cttgaaaatg tcgaattacc gctgatgtat aattcaggca ttacaggcag gctgcgtcgc
    21181 gaaaaagccg aacacatgct ggaaattgtc gggctgaaag accgtttgta tcatgctccc
    21241 aaccagatgt ccggcggtca gcaacaacgt gttgccatag ccagggcctt ggtcaacgat
    21301 ccggttatca ttcttgccga cgaggcaaca ggcaacctcg atacccgtac ttcctatgaa
    21361 attatggctt tgttccagga tctgaacaac cggggaaaaa caattgcctt cgtcacgcat
    21421 gaacccgata tagccctctt cagtagccgc agtatcgtat tgcgtgacgg acggatcgta
    21481 aaagatgaaa aactaagcag cccggcctcg gcagccgaag cgctggcatc gcttcctgtt
    21541 gaaacggaag aaattgaaat ctgatttcag aaaggattaa catgaacatt accaatctat
    21601 tcagaatatc gctcaaggcg ttgcacagca acaaattcag gtcgctgctc accatgctgg
    21661 gcattataat cggcgtggca tcggtcatag ccatgcttgc catcggacag ggctcgaaac
    21721 aaagcattca ggatgaagtt tcaaagatgg gctccaacat gctgatgata cagcccggcg
    21781 ccgacatgtt ccagggagca cgtatggcgg cttcgaacac ccaaacgctg attctttccg
    21841 attatgaagc catcaaaaag caatgtgcaa acctttcggc agtttcgccg atggtggatg
    21901 ccagcggaca agccatatac ggtgcaaata attacccggt cagcatgaac ggtgtaaatg
    21961 ccgactacct gcagatcaga aaatacacga ttgaaagcgg caggatgttc accgataagg
    22021 aaatccttgg atcagccaag gtatgcctga tcgggcaaac cattgtggaa gaggtatttg
    22081 gaaacggcgt ggaccccatc ggcaagacat tccggttcgg caaactgccc tttgaagtca
    22141 tcggcgtgtt ggcggaaaag ggagaaaacg ggatgggcca ggaccaggac aacatcatca
    22201 tcgcccctta cactaccgtc cagaaaaggg tcaaagccat aacttacctg aacggtattt
    22261 ttgcatcaac gacgaatgaa tcggtaaccg aacaagccat ctcggagatt gaatccatcc
    22321 tgcgcacccg gcataaaatt gctgccggcg acgatgacga tttccatgta cgctcgcagc
    22381 aggaaatgct taccatgttc agcagcacaa cccgggtcat gacggtatta ctggccgtaa
    22441 tcgcaggcat ttcgctcatt gtaggcggta tcggcatcat gaatatcatg tatgtatcgg
    22501 taaccgagcg gacccgcgaa attggcctcc ggatggccgt cggggcgcgg ggcatcgaca
    22561 tcctggccca gttcctgatc gaggctgtaa tgatcagcgt tgtcggagga attatcggcg
    22621 tcgccctggg tatcgggagt tcttttgtca ttgaaaatgt aatgaaatgg cctgtcgcca
    22681 taaccagcta ttctatcata ctttcgtttg tagtttgtac atttaccgga gtgtttttcg
    22741 gatggtaccc tgccaggaaa gcggccaacc tcgacccgat tgacgcgatc aggtacgaat
    22801 agcaggttaa tgccgcccgg ggcaaaacgg gacaaataaa aagcaagaaa atgactgttt
    22861 acgataaaaa atattcgctt caaatcctga tccattgcat cgtatgggta attgtcatca
    22921 ccttccccct gattttcatc tataccgaag gtgaaaagga cctggtccga tatctcgatt
    22981 ttacgctccc catcatcatg atgatgattg ttttttatac caattacctg ttcatcatcg
    23041 gtaaatacct ttttaacaag aagatatggc aatttttcct gatcaatgcg gtaatttttg
    23101 ccgtatcgct ggtcatgctc gagctgatga aacagtatgt atgggatcat tatttcagct
    23161 ttcagccgcc aaggcatccc ggcccgccga agaaagacat gatgcgtatc ctcttttaca
    23221 ggaatctgat ttcactggtt tgtacgctcg gggttgcaac tgccatcaag atgacggcgc
    23281 tgtggtacaa caagatggct gaacaaaagg aaaaggaaaa acttcatatc gagacggaac
    23341 tcgccagcct gaaacagcag ttaaatccgc atttcctttt caacaccctc aacaatattt
    23401 actcgctcat ttctataaac caggaatatg cccaggacgc tgttcaccgg ctcagcagcc
    23461 tgatcaggta tgtgctctat gaaagcgacc atccttttgt accgctgagt aaagatctga
    23521 tattcaccgc aaattacatt caactgatga gcctccggtt gccgccgcat gtaaaactgg
    23581 aaaccgacat catgaaacct ccggaatcgg acaccatcgc tcccctcctg tttatgtcgc
    23641 tggttgaaaa cgctttcaag catggcgtaa gccctgaaca tgcttcgtat atttcggtta
    23701 aaattgccaa taaaggccat catgaactgg tgtgcagggt tgagaacagc aattttccga
    23761 aacaggaatc cgacagaagc ggctcgggaa tcggcatgaa caatttaaag aaaaggcttg
    23821 aactcatcta tcccggcgct catacgctgg atatcgccga agacaatgaa cgttatattg
    23881 caaccttaac cataaaaata tgaacctgcg gtgcatcatt gtggacgatg agcccctggc
    23941 gcttgacctg ctggaagggt atgtcgtaaa aactccctgg ctgatattgg aggggcgttt
    24001 cagcagcgct ttcgaagcaa tggctttcct tgaaaaagta aaagtcgacc tggtttttct
    24061 tgatatccag atgccggggc tgagcgggct ggagttttcg cggtcgctgc aaaacgggcc
    24121 ccgtgtaatt ttcaccaccg ctttcgggca atatgccctc gatggattta aagtggaagc
    24181 actcgattac ctgctcaagc ctgtcagcta ccctgaattt ctgtcggctg caaacaaggc
    24241 caggcgatgg ttcgaactca ccgggaaatc ggcaggccaa ctggccagaa acacgatttt
    24301 cgtaaaatcg gactataaaa tggtgcagat agaactcagt aaaatccttt atatcgaagg
    24361 gctgaaagac tatgtaaaaa tctatcgtga agatacggag aaacccgttc tcacgctgat
    24421 gagcatgaag tcgcttgaag agaacctgcc ggagaatatt tttatgcgcg tgcaccgttc
    24481 ctttatcgtc aacctggaaa agataaccac cattgaacgc aaccgcatcg tattcggaaa
    24541 aacctatatt ccggtttccg acaactacaa ggataaattc ctgaagttcg tgaatgagcg
    24601 gtttttctga tctgctctcc gaaaaactct tgtcctgaaa aagaagcggg gcttcgtggt
    24661 caaccacgaa gccccgcatg ttatttcatt gaattcagtt ttatcttatg agcgttaccg
    24721 ttccatgggt ggagttcttc atgccttcat aacttccaac atcgtgtgag ttccatatca
    24781 caccatccct gaacacggcc tcggctttcc acatataaac gccctgctgc aacaacaatc
    24841 cgttgtaccg gccatcccat ccttctgccg gacggccctg atcgtcgagt ttatcggaat
    24901 accacaacag gtttccaaaa cggtcgtata cctgaataag gtattccttc aggttgatac
    24961 ctaccggttt aaacaactgc acttcggcat gcggattttc aggcgagaat gcattcggta
    25021 taaacaaccc tttcaccatg aaattataaa cgtaccgggc cgtatcgctg cactccatat
    25081 cgctgtatgc aatcatggtt ataggatatt ccccgtccag attataaagc acaaatggct
    25141 cttcggcata cgatgtttcg ccgttcccga aatcccagta ataatgggtg gcgcccaccg
    25201 aaatattatc gaattgaagt tgcccctgta cattctcgta atttgcattg tatgtaaaat
    25261 cgcttacagg tggcggatag gtgtttacca attgagtaac tgtatccgtg caggtattgg
    25321 tatcctgaac gatcaggctc acctggtgct ctccggaatt ctggaataca tactcaggat
    25381 tttgttccag cttcatatcc aggagctctg ttccttccct gaaaatccat gtccaggacg
    25441 acaagggggc ataggcgctg tcggaatggt cgaagaacaa cgtcctgttt ccggcgcagg
    25501 agagtgaata atcgtaattg gcttccggca gcatatatac cggtatggtt tttactaccg
    25561 tgtcggaaca tccaagggta ttggatgaaa ttaaccggac atcataataa ccgggcgctc
    25621 cgaagagcca ggaaggattg cgcaacgttg aagtatcttc tgttgaagca ggatcatcaa
    25681 aatcccattg atacccgctg acaattaccc cgttattcac cgtttcgttg gtgaattcag
    25741 ccgtggttcc gtaacaaacc tttccgatgc tgtattctgc aaccggggaa ggactaacga
    25801 taaggtcaac gatcgatgaa tcgctgatgc tttgtccgga tacggccgtg gtcaccctca
    25861 acagtaccga ataattgccg gcttcggtat aaatatggtt gacagggctg gtgtaggaat
    25921 tgtaggttgt cgtggttccg tcgccgaagt cccagtacca ctccgtgatc ggttgcccgc
    25981 aatagctgct gtcggcaaac gagaggttgt aattctggca cacgagcgaa tcctcttcgc
    26041 tgaaaacagc ttcaaggcat ggccttacca gcacctcctg cgaaaataca ttatggcagt
    26101 tattggcatt gataacggta agttcaactg tatataagga aggaaccagg tatttgtggg
    26161 taacgtccgg gttgccggga gcataaatgg tgtcggacag gccatctccg tatgtccata
    26221 tccattcgct gatatctgat ccgttaccgg tcgaaagatc gttaaagacc gctgttgtgt
    26281 cgcacagccc gacggtaaat tcaaaatccg gcagcggcag cggttcaacc gaaaccggta
    26341 tgaaaacggt attattacaa ttgttgatat ctatagcctc caggcttacg gtataaacgc
    26401 cgacctccga ataaaggtgc gtaggattcc ttaaggcaga gttattgtag attcctgaac
    26461 ccgggtcgcc gaaattccag ttgaagaaaa caaggctgtc ggtaagcggc gctatcagtt
    26521 cgggtgaaaa ttccatatag tcgccgatac aggtgggggt atagttaaaa gtaaaatcga
    26581 atgccgccgg aacataaacc gatttgacga tggtatcctt gcatccccgg ttatcggtta
    26641 caattaactg gacagaatag gtagagtcgg atccgtaaaa aacatacacc gggttctgca
    26701 actgcgaata actgcccggg ccaaattccc agagccagga aacgatggaa gcctgcgaag
    26761 cataggagga atcctggaaa taaaccgccc cgttttcgca gggagccggc gtatagataa
    26821 aattggccgt aggattggga aatacctgta ttgtggtgga tgtcgaatcc tggcacccgg
    26881 cagccgttgt aacggtgagc gtcacggtgt atactccctg aaccgtatag gtccattgcg
    26941 gattctgcag gtttgaagta tcgttggcca cagcggtttc cccgaaatcc cagtgccaga
    27001 cagcaatcgg ctccccgttc agggtatagg attcatccga aaactgcgtt gcgctgttga
    27061 tacagccgct gctgaacgag aacaaggcaa ccggttgggc attgatcgtt acggcatgtg
    27121 ttttcgtatt tgagcatcct gccgaatcca cgatggtaag aacaacattg taattgccat
    27181 actgcgcata ggtatgcaaa ggattctgct catttgaatg ggccgaacca tcgccgaaat
    27241 cccaatccca ggaattaacg gcggcaacca tggtaattgt ttcgtcaaca gtgaattgag
    27301 tggccgttcc cgcgcaggta ttcgaccagt tatattccag ggcgggactc gggtttatga
    27361 ttaccgcttg tgttaccgta tcataacagc cgtcggcgtt ggtcacctgc aggcttaccg
    27421 tataggtgcc cgcgctgttg aatatgtgca gcgggttttg caggatcgag gtattgttga
    27481 cgcccgaggt aggatcgccg aaattccaca ggtagccaat aatggcggaa cctccgctgg
    27541 ttgcggactg gctggtgaag ttaacggcca ctccggcaca ggtgttttca taagcaaatg
    27601 cggccagcgg gcttgtttta actattaccg tcttggaagt ctcatcctcg catccgagcg
    27661 acgtatttac cgtcagggtc acggtatagg ttcctgccgt ggaatatacg tgggtgatat
    27721 caggatttgc cggagccgtg atcagcgtat ccgatccgtc gccgaattcc cagtaccatg
    27781 tggcaatagt ccccgaggat gttgtcgatg tattgtcgaa cgacaccggc aggttggcgc
    27841 aggtttgctg agaaaccagg aaacttgctt cgggaggcag cgacacaata gcagtatgag
    27901 ttatggtatt ttcgcatccc gccgtatcgg taattgtcag ggtaacctga tagctgcctg
    27961 atgcactgta gatatggtaa ggatcttctt ctaccgacgt ataggtgtcg ccaaaattcc
    28021 agaaccatga gttagttgca gcagcattta catatgtcgt actgataaaa tgggttgaat
    28081 cgttgacgca gccgggttct atcgaaaaat caactgtcgg atgcggattg atgacaatag
    28141 tcttggtaat ggtatcggta caccccccgg tattggcaac ttccagggta accgtgtagg
    28201 tgccggcggc attgtaagtg tgcgtcgggg atgacaggga cgaagtattg gcggttcctg
    28261 atcccggatc accgaaattc cacagccagc tttgcagacc accggaacct ggttgagaaa
    28321 gatcgttgaa ctggatttct tccccctggc agtgtatggc atcatagctg aaattggcca
    28381 gcggatttgc cgaaaccgtc actaaacgcg atgctgtagc cgtacaactg tcggtggttt
    28441 ttatggtcag ggttacattg aaatttccga aggttgtgta tgtatgcgaa atattgggat
    28501 tgccggggtg attaatggtc gttgtgttgc catccccgaa attccatacc cattgtacaa
    28561 tataccccgg cgctccgcca agattggcaa ggcttgtgaa ctggatctct gaatctttac
    28621 aggcaggctg gtcccatgta aaattggaaa caggcgccgg gttgattacc accggatttg
    28681 tgacaaaatt cgtacacccg ttggtatcgg taacggtgag cgtcacataa taggttcctg
    28741 aagtagaata aaggtgggac gtgtttgggg aagcggtatt aaacaccggc gatccgtcgt
    28801 taaaatccca ggcataattt gttatggcgt cggtatttac aatattcgta tcggccataa
    28861 acaggacctg gttgctcagg cagttgttct gtgaagtaaa agcaaccgcg ggtttggcat
    28921 tcacggtcac attaatgctt gtggtatcac tgcacccgtt gcttgtttcc acaatcagcg
    28981 tcaccggata ggtcccgggc cctgaaaagt tatgggccgg gctctgaatg gtggaataat
    29041 tcgcagtgcc gctgagcgga tcgccgaaat cccagttcca tcctaatatt gaaccgcccc
    29101 cgtttgattg cgacagatcc gtaaactgca acggataacc ttcgcaggca tggttatgat
    29161 cgtaatcgaa attcgccagc gggtttggat agatggtgat cgtctgggtt tcgaaattgg
    29221 aacaactgtc agcggtataa atggtgagtg taacaagaaa gtttccagga agggcataca
    29281 catgttggac attgggatta cccgaaagat caacagtatc gatggttccg tcaccgtaat
    29341 tccatatcca gcgccagatg tagctccagg aagtacttga taagttagta aaatttaccg
    29401 tagctcccga gcagttgttg cttcccggat caaaatgggc tgtcggcaac gggtggatgg
    29461 ttatcagatg ggatatggtg ctgtcgcatc ccagggtatc ggtaacggta agcgaaaccg
    29521 tataatcacc gggagcaata taggaatgtg ctgtattttt agaaggtgaa gttcccccgt
    29581 cgccgaaatc ccaccaccag gttgatattg ccccggcatt catggcgttg gtatcgggat
    29641 taaaatatac caggtcgtta aggcaggcgg atgtccaggt gaattcggcc gccggatgat
    29701 cgtggatcac gatctgtttg gtcatggtat cggaacaatt gttaaaattg gtaaccgtca
    29761 tcacgaccgt ataggttccc tgcgtgttgt aggtatggat cgggttctca agattggaac
    29821 tgttgttgtt gccactcatc gggtcgccga aatcccagga ccatgctaca ttattcccgc
    29881 ttccaacggc agaagaagca tccgtaaact gtatgacctg gtcctcgcaa agtcccgtct
    29941 ggctgtaatg aaaattggca atggggcttg acattaccgt aaccggaatc gtaatgctgt
    30001 tgatgcaatt ccgggaattg ataaccgtca gcgttacatt ataagtggtg ggattgggaa
    30061 aaatatgatc tgcgttaggg tcatcgggaa aatggatggt atcgttcggc gatccgtcac
    30121 cataatccca tatccaggtg gtgatatagt caagtggggc acttgacagg tcggtaaaat
    30181 gcacggagtc ggcctgacag tttggggttt cataactgaa aagcggcgtc ggaagaccat
    30241 aaatctgaac gtttctggta aaaatccccg tgtttccgtt tatatcggtt accgtcagga
    30301 ttacctggta aatcgttgca cctgcaaatg tatgcgatat cgattgcccg gattcaaagg
    30361 taccatcccc catgttccac acccaggaag taatgatgga aggatcgggc accgtcgcgg
    30421 tgaacagtgt cgggctgccg acacattcgc ccgaatacga aaaatcaacc gttatcggtt
    30481 gggctgataa gggttttata aaactattcg ctgctgaaaa cagtgccgtt atcagtaatg
    30541 ctaataatat cctatgtttc atcgaggtat gtatcgcttt gaggggctgg taatatttag
    30601 actggtcagt atatgcagtt tattcaatag tcaaaaatag taagttttta ctatccgtga
    30661 cataactttc aatcagaact gtaaaaaaaa cggacctgtc cggataaaat tgccggaaaa
    30721 agtctcttgt attgaactca tctgaatatt aatcgtctgc cggcagaaaa gtaatgcgat
    30781 tcggcggagg aatatctcac tgaattacct cgttttgacc aatacaaaga tattactttc
    30841 aggatttgga tttcatgaac aggcctgatt ttcaccggta tcggttcggg cgcctttcaa
    30901 ccggtaaagc ctgcgtcccg ggctttcttc cggcgcccgg cgcaataaaa aagaggctgt
    30961 cgcaggttca ggacatgttt tgccatttaa accaatgcaa aacagcatat ctgcgacagc
    31021 ctcaatcagg aaaggtccgg tttctatttc ttttcagccg tattacgctt cctccatgcc
    31081 atgaacagga accaaagccc gaaaatgagc atggccagtc ccgtccataa attgacattc
    31141 atccccagcg atgccttata aatttcggca ttgccggtaa cgccgccata aatggtgagt
    31201 atggcaccca gtatagtgaa cagcgaacct attggaaatc tgatatcgtc acccattgat
    31261 ttttcctcct accataaaat gattgttaaa acaatgctga tcgccccgac gacgatggcc
    31321 agtacaaccg gcttttcgta ccacggaatt cccggttcat ccttaatgcg aggcgtaagc
    31381 gaatacacca gaccggtaag gtcttccctg gttttctgct gacgcgtgaa cagggagatg
    31441 atcaccgtga ccaggaagca aaccgagaaa gcaaagattg ccgtccagta gctttgcgcc
    31501 atagagctgt aatactcgtg gatattcgcg atccaggcgc cttttccttc ggccagggta
    31561 aggccgtgat gaagggaagc agccacggtt ccagatatca atccccagaa agcgccgtgg
    31621 ccggtagccc gcttccagaa catcccgatc aggaagaccg cgaagagagg cccgttcacg
    31681 aaggcaaaga tcagttgaag gaaatccatg atgttgttga acgatgcagc cacataagcc
    31741 gtcaggatgc tgacagcgac gccgaaaacg gtggcaaact gccctacatg caggtaatgc
    31801 tggtcgctct tgcctttgtt gatatacgac tggtagatgt cgtaggtaaa cactgtattg
    31861 aaggccgtca cattccctgc catcccgctc ataaaggaag ccatgagcgc cgtgatcccg
    31921 agcccgagta aaccgttggg atagtaatgg accagcatgg aaggaatgat catgttgaag
    31981 tcgttcggat cgccgcctat cagttgaaaa ttcttgtccg gcatcatcat caccgccgcg
    32041 gcgatcattc cgggaacaac aacaatcagc ggtataaaca tctttggcaa agcgccgatc
    32101 agcggggtgc ggcgggccgc cgacatggaa ttggccgcca tacccctttg gacaaccagg
    32161 aagttggtgc accaataccc gaatccgagt acaaatccaa gtcccatgaa cattccgagc
    32221 cagtcgacgc ccaaaggatt ggattcaggt ttaccgagat acttccatgt atgcagccag
    32281 gtgtcgggtt caaaaccttt ggcgccggca accaggccca ggctttcttt caaaccgctg
    32341 taaccgccaa cgtcgttcaa accaatgatc acaagcggca acatgcctgc cacgatcagg
    32401 aagaactgga gcacttcgtt gtaaatggct gatttcagtc cgccgagata ggtgtagatc
    32461 agcacgatga ccgccgatgc gatcaggctg acgtgaaagt tccagtgcag cagcacttcc
    32521 atcagccggg ccagtgcaaa catcgagatt cccgaagcaa aaaccgtcat gatggcaaat
    32581 gcgaacgcat tgaagcccct ggttttctca tcgaagcgga atttcagata ttccggcacc
    32641 gagcgggctt tgctcccgta ataaaagggc atcatgaaaa tgccgaggaa aatcatgccg
    32701 gggatagcgc cgatcaggta aaagtggcag gtaagcatcc cgtactttgc cccggaggcg
    32761 cccataccga atacttcgag ggcccccagg tttgccgaaa tgaaagccag ccccgtgatc
    32821 caggccggga tggatttccc gccctcgagg aactgcgccg acgatttcat gtactttttc
    32881 agcagccatc cgattcccag cacaaaggca aagtaaatcg ccatgatggc atagtcgata
    32941 aaattcagtg aaaaattttc agtcatctgt tgaacttatg atttcggtgt ttgattttat
    33001 tatttcgtaa aggaatggtc atcaggaatc agcaagtcag gttgtacagg cgtcgattca
    33061 gaaagattgc cggcgaggcc ggcggcgggc cgatggcctg tcgtcttacc cacaaccttg
    33121 cccaggcagg taaacttttg gtcgttagct attagcctgc caactcacac acaatccaac
    33181 ccaggcaggt aaacttttag cctttagcgt tttgtgtttt gtgttttgcg ttttgtgttt
    33241 tgcgttttgt gttttgcgtt ttgcgttttg cgttttgtgt tccgtgtcac cgttttccgt
    33301 cctccgtact ccctgctccc tggcttttgg ccgttagctt ttagctttta gctattgtct
    33361 gttagctctt tcattttggg ctttgggatt ttcattctcc gttttccgtt ttgtgttttg
    33421 cgttctgtgt tctgcgttct gcgttttccc ctgcctgcca cctccaggct cgcgcaaggc
    33481 aggcaggcct gcctacctct cacactatgc cattcaccgg caggtaaagt tttccgttct
    33541 ccgtcctccg tcctccctgc tccgtcctcc ctgctccgtt ctccgtcctc cgtactccct
    33601 gctccctggc ttttggccgt tagcttttag ctattagcct gccaactcac acacaatcca
    33661 actcaggcag gtaaactttt agcgccttgc gacctgcgcc tcgctccttg ctccttgctc
    33721 cttgctccgt cctccgtcct ccgtcctccg tcctccctgc tccgttctcc gttctccgtt
    33781 ctccgtcctc cgtcctccgt tctccgttct ccctgccccc tgttcctatt ccctaccctt
    33841 tttccatctc cttccatacc agcgcgctgg ccccgacgat ggccgaagtg cctgctttga
    33901 gttccgacgg gagtattttc actttattgc ggaacacggg cagcagatgg cgttccatac
    33961 tttcccgggt gggtttgaag atataatcgc cggcggcggc ggctcccccg aaaaggaaga
    34021 ttgcctcggg gctcaggtga tggaccgtat cggccaatcc cctgcccagc agttcgccgg
    34081 ttgcttcaaa cacttcacgg gcaatcgggt cgcctttttc ggcagcatcg aaaacgattt
    34141 ttgaagtcat gtcgttaaag ggcaccctgg ccagttcgct ttccgaattg ttgtatttag
    34201 ccagcaattc ataaacggta cgtttgatgc ccgttgccga gcaataggcc tcgaggtggc
    34261 ctgctccgcc gcaaccgcat acccttccgc ccggaacaat ggtggtatgg ccgcactccc
    34321 cggcaaagcc gtcggcgccg taaacgaggt cgccattcac gataagcccg ctgccgagtc
    34381 cggtccccag ggtgatcatc acgaagtttt tcatcccttt tcccccgccg tagatcattt
    34441 ccccgagagc ggcggcattg gcatcgttgg taagcttcac gtatttgata tcagggaatt
    34501 tctcttcgat gcgctttgca aaatttacaa cccccacgaa aggaaggttc ggagcgtatt
    34561 cgatagtgcc gctgtaataa ttgccattgg gcgcgccgat gccgataccc agcacttcca
    34621 gatcggggtt gatctggcgg acggtatcaa tcgccgacct gatttcaccg gtcagggatt
    34681 ttatgtattc cgaaagcaat tcttcagagc gcgccacatc ggcaagcggg ttctgatttt
    34741 tctgcggagt aggcaactgg ggctttttct cgtaaagcac attaccatcc ctgtcgacca
    34801 ctccgatggc agtatttgta ccgcctatat caatacctat tgcaacctgt ttcatggtat
    34861 cttaatggtt tgtcgttgtt tattagcgga ttttgcttcc cttaagggca aagaatgtta
    34921 tgtaagcata acaaatgatc ggaatgataa gggcgcccct tgcgctgaac gaatcagtcg
    34981 cggctcccat cagcagcgga accacagcac cgccaacaat cagggtattg ataatacctg
    35041 cagcaatagg catttcggaa ggatccaggt cgctgacgcc taatgagaat atattgggga
    35101 acatgatgga attgaagaaa ccgatcgaaa gcaggcacca catcccgaga tcagccggaa
    35161 gaaggtaagc tgcaactatt aatatcatat tgaccgtccc gaaaatcccg agcgcaacat
    35221 tggctttgcc ttttccaagc agcataagga agaagttgat aactgcaatt gcaaagaaaa
    35281 tgagaccatt cagcggttgc gattcaaaaa caaattttcc gtcagtaaca gacgaagagg
    35341 taatgaacca accgacaaag aaggcaaaca ccatcaccag cgccgaatac tgatatttcc
    35401 tggcattctg tattttgctc agcaatattg atccgaagaa acgcccgatc atggcgccac
    35461 cccaataaat cgccgaaagt ttcacagcca cgtctatacc gaaaccgctt tccttgagat
    35521 aaggaggtat ctgacccgcc gtacctactt ctgcgcctac gtaacagaaa attccaattg
    35581 cgccgagcca aacctggggc ttcttcagca ccgattttga aggaatcgtc ttatcacccg
    35641 taataacagg aagcttcaca aaagccagtc cgatgcccac cagcagcaca atgctcccga
    35701 taacgataaa aggcatctgg acaaatttgg cagcttcagc cggcgttgaa ccattgctta
    35761 ttgccatcac agccggtgac agaaccaata taccggccag gaacggggca atggtagtgg
    35821 caagcgaatt cagggcctgg gtaaggttga gacgtgctgc agacgtttcc tgtgacccaa
    35881 gtgcggcaac atagggattt gcggccgtct gaaggaaaac cacgcctgtc gccataacaa
    35941 atactgcaac caggaacaaa gggtacgaaa ctatttcaac ggctggaaaa aacaggtaac
    36001 tgccaaggcc ggccagcacc aatccagtaa tcacaccact cttatagcca attttcttga
    36061 taattccgcc acacaaaaaa ctaaagcagg cgtatgcgaa gaagaatacg ccgtttacca
    36121 actgggcgag gaaattttcc cagcccgggt acaacgtctt caggtcaaat gcaagttgca
    36181 cctgatcctt catggcaata ttaaagtttg tgaggaaacc gaagataaag aagatagctg
    36241 ccataaatga cattcccaac ttcaggttgc tattgttttt ttgttccatt gtgcatttgt
    36301 tttaaaggtt aattaaagcc gtttttttgt cgggaaacaa atgtaaaaat ttaattcagc
    36361 tatggaatcg caaaaaagat tgattgataa aatattcgtg atacctgtta aaataatacc
    36421 aggcattccc tgaatattat ttaatagcat ccaggcaaac aataacataa ttggctgtaa
    36481 aactgcaact ttaatttctt accacgtatt cgtttccggg tattccggaa taaaataaaa
    36541 cctgatgaaa tttattcgtg taacaaatac atttatattt gtccggctga gaatcaagat
    36601 attatatcag cctgaaaacc aaaaatttag actgcaattc gttcaaaatc atgaaaaaag
    36661 cttcaaagat caccggcctc gcgatggttt ctgtccttat agtgacgcta gctgccggat
    36721 gctgtaaaaa aatccagacc atgaacatta ccaaagaagc gttcggtatg ctggccgatt
    36781 ctgtaccggt atccttgtac acccttacca acgataaagg cattacactg aaagtcacca
    36841 attacggggg catcatcact tcgctgttgg ttcccgacaa agatggaaac gaaggcgata
    36901 tcgttctcgg atatgacagc ctttcgggtt acctcaccaa aacgccctat ttcggggcta
    36961 ttgtcgggcg ttatggaaac cgcattgcca aaggcgtttt caaaattgac ggcaatgaat
    37021 tccgcctgcc ggtcaatgat gggcccaacc acctgcacgg cggtatccgc ggatttgaca
    37081 aagttgtgtg ggacgccaaa gaattcaaaa ccgattccac tgtcggcctt gttcttcact
    37141 acctgagcaa agacggcgaa cagggctatc ccggcaacct tgacgtaacc gtcacctata
    37201 ccctcaacaa taacaacgaa ttacgtttcg attacctggc caccaccgat aaaccgacac
    37261 cggtaaacct tacgcaacac agctatttca accttgccgg cgatggggat atcaaagggc
    37321 acgaactgct tataaaagca tcgaaataca cggttgtcga cagcaccctc attcccaccg
    37381 gagaattgcg cgacgtaaaa ggcaccccgt ttgattttac gacagcaaaa accatcggca
    37441 aagatctggc agccacagga ggtaatccga caggttttga ccataatttt gttcttgata
    37501 ccaaaggcct tcaggaagcg gcaatcaaag ttactgaacc cgcatcggga agagtgatgg
    37561 aagtatttac cgaccagccc ggcgttcagt tctatagcgg aaatttcctt gacgggacca
    37621 taaaaggcaa aggaggaaaa gtctataacc agtacaacgg tttctgcctc gaaacgcagc
    37681 atttccccga ttcccccaac cagccggcat tcccgaatac catactcagg ccgggcgaaa
    37741 aataccagac cacgacgatc taccggtttt caaaataaaa tccctgatgg ggacaagcct
    37801 gcgacttacc ggtggtatga tcggcgttcc gtgaatttac taaccgatcc ttccggcgcc
    37861 gttcccgacc gaagctacat agaaatcggc tgagaggcct gttgtggcat gatacaccgg
    37921 cccaacacag gcaataagct catcaataga atcgtcatca acaatggcaa cggcgcagcc
    37981 gccaaagccg gccccggtca tccgggcgcc gacaacgcct tccactttcc aggcctcatg
    38041 aacgatggta tcgagttcct tgccggtaac ttcgtaatcg gtgcgaagcg attcgtgcga
    38101 agcgttcatc agcttgccca gtgttgtaag atcgcctcgt ttcatggccg ccacggcatc
    38161 aagcacgcgc ctgttttcgc tgatcacatg gcgggcgcgg tttcggatgg ttttatcggc
    38221 aattactttt tcgttttcca gaaattcggc tatttccata tcgctcaggt cggtcaacgg
    38281 ccggatcgaa cgcaggcttt ccagggccga agcacattcc ctgactcttt cattgtattt
    38341 ggaatcggcc agttcgcgtc gtttgttggt attcatgatc acgatgctgt atccttccat
    38401 ttcaaccgga acgagttcat attccagcgt cctgcaattg aggaaaatcg ccgaactggc
    38461 tttccccata ccaacggcaa actgatccat gatgccgcaa ttcatgccta caaactggtt
    38521 ttcggcggca cgtcccattt tgatgagctc gatgcgatca ataccgctat ccagcatttc
    38581 gtcgagcgcc accgccgtca ccaactcaat ggaagctgag gatgaaagcc ctgccccgtt
    38641 ggggatatcg cccgaaaaga gcatatcaaa cccttgcaat tcaacacctt tcttcacaaa
    38701 ctgatcgata acaccaagcg gataattcac ccattcgctg ccctgttttt tggccaggtc
    38761 gccaaacggc acatcagcgg catagctaaa attgaaagtt gcaaaccgca tccttctttc
    38821 accgttcgga cgaacagcta ggtaagtccc gaaactgagg gcgcacggta atacataccc
    38881 gccgttataa tcggtatgtt cgccgatgat gttaaccctg ccgggggcaa aaaacaaggc
    38941 cggggcttcc ccgatgccgt accgtgactt aaattccttc tgtaatgctg ctatggccat
    39001 ggaagtggtt tttgaattat taccggcaaa aatagaaaga atctgatacg aaatggcgac
    39061 ttttgcaggc agccggcaag tcacctctct ttgcctgtat ctaaaaaagg aacattcatg
    39121 gagatcaggg gaaaatattt cattctgaat ggaaaacagg agccggctga agtctttcag
    39181 cagctttctg caatcgaagg ccctacgtta tacgaagttt tccggattat caacggtatc
    39241 ccgctgtttg ttgaagatca tatcctccgt ttcgaaaaca gcgccgggct ggcagggctt
    39301 gtcttgcctt ataccaggga tgatattttc aggcatgtta caattctgat tcgcgccaac
    39361 cggttcagga atgcaaacat caaactcata gcctgttatc cttcgggaaa gccgccctgt
    39421 tatgccgctt acttcgtcga gcatcaatat ccaaagccgt cggaattcag gctgggcgtc
    39481 gataccatta cgctcagggc tgtcaggcaa aacccgaatg ccaagatatt ccatactgct
    39541 ttgcgtgaag aaacggatgc acttaagaag catctcaagg tttacgaagt attgctggtt
    39601 gataacaacg gaaatattac cgaaggcagc cggtccaatt ttttctgcat ccgcggcaat
    39661 caggtcatca ccccaccggt acacgaagtg ttgccgggtg taacgcggaa atacgtcctg
    39721 caagcctgcc ataacctgaa atgccctgta tcggaaacca gtgttccggc cggcgatctg
    39781 ccttcgatgg aagcgctttt ccttacagga acgtcacgaa agatattgcc gatcagaaag
    39841 gtggacggcg gctctttttc cgtcagtcat cccatgatgc gcgccctgat ggaggaattc
    39901 gggcggatcg ttgacgatta cctttcgcgt catgacaagc tttaagatgt tcaggcatgg
    39961 tggcggccgg cggctagctg ttagccgtta gctcttacgt tttgcgttct taattttgcg
    40021 ttttgcgttt tgtgattagt aattcgtggt attcgttata gcattcgtga aattcgtgga
    40081 cagtgtttca gcgtttcagt gtttcagagt ttcaga
//
LOCUS       DAOPYV010000025        39779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_887_length_39779_cov_12.610538, whole genome shotgun sequence.
ACCESSION   DAOPYV010000025 DAOPYV010000000
VERSION     DAOPYV010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 39779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39779
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_887_length_39779_cov_12.610538"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PKI35_04885"
     CDS             <1..665
                     /locus_tag="PKI35_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNX43753.1"
                     /translation="NVVNPDDLIEKYGADTLRLYEMFLGPLEQSKPWDTSGVEGVSRF
                     IRKFWRLFHNEKLEFSVSDEVPTKEELKILHKTILKIQEDIERFSFNTAVSAFMICTN
                     ELTDLNCNKRAVLADLVVLISPYAPHIAEELWHLLGNTGSVCNAKFPEYNAAFTVDNS
                     FTYPVSFNGKTRFMLELPLDMPVADIEKAVLTAPESQKWLGGNPPRKVIVVPRKIVNV
                     VI"
     gene            complement(686..1003)
                     /locus_tag="PKI35_04890"
     CDS             complement(686..1003)
                     /locus_tag="PKI35_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HNX43754.1"
                     /translation="MTSTTFEIPGYTIVKNLGLIRGIIVRSRSIFGTIGAGLQTLVGG
                     NISLLTELCEKTRADAYMEMMVHAEVLGANAVIGIRYESTEIMSGVTEVICYGTAVLA
                     EAQ"
     gene            1145..2044
                     /gene="rarD"
                     /locus_tag="PKI35_04895"
     CDS             1145..2044
                     /gene="rarD"
                     /locus_tag="PKI35_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HNX43755.1"
                     /translation="MPDNRNSQYFSGLMLAVLCYTCWGIFPVYWKLLKEVPSDQILVH
                     RIVWSFIFLGILLAFLRNRQVIRYLKDIRIAGLLLMTGLLVGSNWGVYIYAVNHDRIV
                     DTSLGYYMNPMVNVLLGVIFLKERLSRIQKLAVLFAVGGIVWLTLYVGRLPWISVYLA
                     LSFGAYGLLRKKANLESLPAVFIETMLLTPLAIGYLLFAEYQGTGAFLHGSWLISVLL
                     IIGGPVTALPLYWFGKAATKIPLSTIGFIQYLSPTIALLIGIFVYREPFRIPYVVAFA
                     MVWTGLALYTISILRDYRTRRLS"
     gene            complement(2188..3633)
                     /locus_tag="PKI35_04900"
     CDS             complement(2188..3633)
                     /locus_tag="PKI35_04900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNX43756.1"
                     /translation="MKSQNFPFRVLTFGIILILILPALLKDGMFMDGLQYACVSKNLA
                     HGQGTFWQPHLSNTWNKEGNHAFLEHPPLVFGIQSLFFRALGDSRYTERIYSFLTALI
                     TAWLIAMIWRFVFRKEEKIRNSEWFPVLLWIITPVCFWSYRNNMQENTMGIFTLAAVY
                     FSLRAVEKSRRYLPNLLLAGIFTVAAVLSKGLPGLFPLAFIPVYKALHKDFTLRKMLL
                     YWLIFLAIVAAGFSLILLIPGASESLRFWLFERVFSRIESEVTTSNRFDVIWRIFTEL
                     IPALLIAGGLILTGKFRKYNPGNNYRKQALLFIIMGLAGSLPMLFTLVQKGFYLVPVF
                     PLFTLGLAAISVPFMVHFTEKSDPASGSFRAFRLITLVFLAGILVFSAMQINKTCRDE
                     EVLSDVYRLGSVIPEHSTAGINRTLYANWPLQLYLLRYYDINVDPSPVESPDYFILEK
                     KDTLPAAAGYRREPLNTLKFDLYRLSAAGSR"
     gene            complement(3661..4590)
                     /locus_tag="PKI35_04905"
     CDS             complement(3661..4590)
                     /locus_tag="PKI35_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNX43757.1"
                     /translation="MEYIGIQGQIRKNNLNSVLLLIAFPVLLLAMVYIFLFFTSGHRA
                     PGANAAFIAAVPYVLAGTAIWFLAAWFAQSSLIRLATGSKPLERRENKRVYNLLENLC
                     IANGMKMPRLYVIEDDSLNAFASGINDSTYAVSLSRGIIENLDDEELESVIAHELSHI
                     RNRDVRLLIISIIFVGIFSFLAEMAFRSFRFIGRGSKSNGKNGGMIILIAVVLTAICY
                     LVSMLLRFGVSRSREYLADAGSAAMTHKPYALASALRKIANDPAIEAVESRDVAQLFI
                     ENPKPSAHGFSWDNLFATHPPVRKRIELLEQFV"
     gene            complement(4596..5156)
                     /locus_tag="PKI35_04910"
     CDS             complement(4596..5156)
                     /locus_tag="PKI35_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNX43758.1"
                     /translation="MAIIVILAIAAILVLWLISLYNRIIRLRNRRENAFADIDVQLRQ
                     RHDLVPQLVETVKGYAAHERELLTRITEARAAAIQAGTIDEKIKAETTLGNALQGLKI
                     AVENYPDLKANQNFLQLQEELSDIENKLAAARRFFNAATAEYNTGIETFPSNIIASNF
                     GFKRETMFDPGPEKRGQLEEPPTIKF"
     gene            5713..8349
                     /locus_tag="PKI35_04915"
     CDS             5713..8349
                     /locus_tag="PKI35_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNX43759.1"
                     /translation="MDTKTNINGIKYTDALYADEYPPLVAYFLIDTSGVVLDFDAQTA
                     AILNVQGPSFKGVNLLLQAGDKSFVHSLNEALSGKTTYHSGQISLAGREPRKNFATYL
                     KPVYYPSDKLIGVAGFIHYARPGIVSPFEMGRQAGYLNLIAENTHDIISLHSFDGKIL
                     FLSPSFEQFTGIPYDKAMQDSAVWPVYPDDKQIVLDAIAGMKDGSYPVNIEYRYIKCD
                     KSIGWAESKFQIISNSGMHDCVAAVTRDITARKAAEDALIRSEIKYRNLMLNLPTGVV
                     LLNLNGKIIEANDAFFKIIGKPSSELGNLLSIKEINQKYNCNVSAEFNLCISEKKPVE
                     GQQELATPGNIKRHIVYSLVPLTDPSGKVSAVMGNIRDHTDLITAEEESRQQVEFLNI
                     IINTLQEPFFVKDEHHKWVMMNDANVEMIGHPRDFLIGKTDYDIFPKEQADVFWKMDD
                     LVLENGANTNEEQITWSTGEIRDAITSKYLYHDKFAGKKFIVGSIHDITHLKKVQDTL
                     KNSEQKYHDLFQNANDLIFTIDLKGNFTDANQRVIDILGMPLTQVLATSVFDVVKNIE
                     IGQFKEILKKLLKDKSVPAYEVEVQSASGSTIVLEVRSRLMYDNNDTPIGITGIGRDI
                     TDQLTYNQRLNQYNDELKELNKSKDKMFSIIAHDLKDPFNSLLGFSDILLEDFDSLDR
                     DEMRDYIKIINNTAKHSLNLLENLLTWSRLLTGRLPFTPMKLMLADEVETATTIVSSL
                     AYRKRITVNNRVPSDMAVNADQYMLLSILHNFLMNAIKFTNPGGEINIAAEIESQSCG
                     EDPEVCISITDNGIGMTADEMSKLFKINLLHSTTGTQNEKGTGLGLLLTREMIERHGG
                     TVHVKSEPGRGSTFSFTLPADL"
     gene            8813..11215
                     /locus_tag="PKI35_04920"
     CDS             8813..11215
                     /locus_tag="PKI35_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNX43760.1"
                     /translation="MAPDSAISDFSGIVSDYFWEHYYDAADDFIAVIDTSCRFVRMNS
                     LLSEKIQPFGIEAGGMCEICSRESKEFKLQCPVWQALKTGKEHSGEFDDSRLGNIFIR
                     ALPFFGPDKSVSGVLMVASDSNKREVSPISTGLLGRDPQSIINAMPEAILICDNSGMI
                     LTASNKLRELFVLPANKSLDGSNFLSFLMPGYIESSLTDFLTVANGTKNFIKGKYRLR
                     RTTNEGFWAEVLFSPLKDSRNDQSGMLIEIRDISDTLQAEESLNKNYIRLTRLHKTFT
                     GFSPDSEKNILQLIALYGELLGASVCSYYRIANGHSQLVALWDSPLITGVMPDTAYAD
                     IVKQYSSGRENFLVINKPFNFSDAGFGSFIDDNGITMIVVNPVRNNNKLVGLISAFFT
                     HHYTLRNEDKEFCNIIASATAVEESRISTQKAREESEMNYRELSDTISDAIYVMDPDG
                     KVIDVNLGAIGKYGYTREELIGSTPRMISAEGRNDLELFNEYLKKAFKGETINFEWWG
                     KRKNGEVFANDVVLNRSRYFGRDVVLAIGRDITERKQAEEKLQQYNVELRETNASKDK
                     FFSILAHDLKNPFQGLLGFIDLLVEDIDELEKQQVKDYLQNIRSASYQTYSLLENLLE
                     WSRIQTKKTPFLPTVFELSAEIEEIFSLLTGNAMRKGIKLISDVPAGVFVEADRNMAH
                     SIIQNLVSNAIKFSNSYGKVTVESSIVNSPNPGKEPVDGRKWLLISVLDNGVGIPDDI
                     MAQLFKLDGQVSMVGTANEPGTGLGLILCKEMVEKNGGKIWVNSESGKGSIFSFTLLY
                     HE"
     gene            complement(11265..13916)
                     /locus_tag="PKI35_04925"
     CDS             complement(11265..13916)
                     /locus_tag="PKI35_04925"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNX43761.1"
                     /translation="MHRFLSLLITIGFIMITTVAEAGSFFSGSNPDTLAVTHDSSFSL
                     PPDTLSRQDTLSRPDTLKKKPQMIEDIIRYSSSDSITLDLKQKLAYLIGNAHIEYGDI
                     KIDAYKFIINFNNSSIYAEGTTDSTGAAVGTPVFTQGNLSFTSKTLTYNFETKKGIIQ
                     NIVTREGEGYLQSNVVKKLADGSTNSKQGWYTTCENTDHPHFAIKYSKARIIPDDKIV
                     TGPAYLSIEDVPLPLVLPFGIFPSKKGRSSGIVIPKYGESANRGFFLENGGYYFGLND
                     YFDLKLLGDIYSRGSWAVKPTFTYKRRYKYSGSFNLNYARNVTGVKGSADYATSKDFF
                     VTWSHSQDSKANPNSVFSASVRAGSSKYNRYNNPLSVNDYLTNTFSSSISYSRKLGEI
                     GNITASARHSQNTTNGSVSLSLPEVALTMNRFYPFKRKQATGPTRWYESITVTYNMNA
                     RNDLNTVDTALFTRQSLDRFMMGMKHSIPVQGSFKILKYFTWSNGINYNERWYFKTVR
                     KSYISDTTYIAGDTIAPHVQTDTVSEFRAARDYSFNSSVSTTLYGMYQFGKKSPVKAI
                     RHVVRPSVGFNFVPDFTTGKLGYYRTYQDAEGEEVLYSIFGESTFSPYGSPPRGKSGS
                     INFALGNNLEMKVRSKKDTVNGTTKVMLIDNLNFSTNYDLAKDSMRWSNLNISGRTTL
                     FKKIQVNYSSSWSPYAVDSVTKQAYNKFEWEVNRKLFRFENSAWSLSLGYDFRSSDKM
                     KKKTETGKGTADEMEYIRNNPEQFIDWDNPWNLRINYTFRYTGVLANRYTGEKKKTVY
                     QTIDFNGDVSITQKWKVTLRSGYDFKNKDFSLTQVSIYRNLHCWEMSFNWIPYGAQKS
                     WSFAINVKSSVLQDLKLTKKKDFRDSL"
     gene            14115..15308
                     /locus_tag="PKI35_04930"
     CDS             14115..15308
                     /locus_tag="PKI35_04930"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNX43762.1"
                     /translation="MTEKHRSPAFRIVLFLLLASAVAYGQKVSTVVIDAGHGGHDTGA
                     LGKNSREKDVALSIALELRDYIRQNLPGVKVVLTRDKDVFVELYRRAKIANESNANLF
                     ISIHCNSTKTQSVQGTETFVMGLDKTGANLTVAKAENAAILLEDDYVSRYDGFDPGSP
                     EGNIFFSMMQNAFLNNSLDFAGRVQANFRDRVKMNDRGVKQAGFLVLYKTAMPSVLIE
                     CGFLSNSSDEKFLLSPKGQAQIAYAIYKALADYKNGVEKNGGPLITARDSTPVQRTVK
                     EKEVMPVIAEEVKTTAPAVAGNDNAGTPVPAGAVRYRVQFALSKTQMPLDSPEFKGIE
                     NVKSYFHNGMYKYTAGDKPSLEEAMQLRRSMVEKGFKDAFVVPFLDENRITNEEAKRL
                     TGNLK"
     gene            15335..16327
                     /locus_tag="PKI35_04935"
     CDS             15335..16327
                     /locus_tag="PKI35_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNX43763.1"
                     /translation="MKIRRDVKIGVFSASVILLFFLGINFIKGKDMFRKHRKFYARYE
                     HVAGLSSAASVSVNGLDIGKVSDVHFAGDASNNVIVEMSIYNNIQIPENSVARIFTPD
                     ILGTRSIEIVIGDSPIMAENGDTLISEMQATLSEEVSQQVAPLKRKAEGLMGSIDTMI
                     TVVRSVLNLETRENLNASFQHIRQTIFALEHTTYNLDTLVYGQRTRLERIFYNVESIS
                     ENLRQNNDKITNIIDNFSAISDTVARANLSKTLLDLQYSVTSVSMAMEKINNSEGSMG
                     LLLNDKKLYNDLESAGKELNSLVTDIKLNPHRYLNFSVFPPGGKRMKYKEPEGK"
     gene            16392..16763
                     /locus_tag="PKI35_04940"
     CDS             16392..16763
                     /locus_tag="PKI35_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNX43764.1"
                     /translation="MEINQITEKVIACAIEVHKRLGPGLLESVYEECLSYELMSAGLA
                     IERQVPVPVVYKEIKLECGYRIDILVERTVLIELKAIEAFAPVHEAQILTYMKFAKKS
                     IGLLINFNVTLLKDGIKRYRY"
     gene            complement(16879..18402)
                     /locus_tag="PKI35_04945"
     CDS             complement(16879..18402)
                     /locus_tag="PKI35_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNX43765.1"
                     /translation="MSSLKFPIRFLLAATIMLAAGMLHAQGTFRIRIVGAGALAGNKL
                     IGENIVRAIGHPVFEHAGAYLYCDSAWLDEKTNNVDCYGHVRIKSSDTLNLYGKFLHY
                     DGNTRIARISDDVKLVDNQTTLTTNYLIFDRNSQIAYYNSGGKVVNKNNTLTSKKGDY
                     HTDTKMMFFKEDVVMKNPDYTINCDTMKYNTQSRICYFEGPTIIRGKESYLYCENGEY
                     RTITGQSCFSVNALLVDEGRRLTGDSLYYNEVGKNGKAVQHIILTDTAQNLIVTGNYA
                     EYFRKQGFAMVTDSAVARLGDEKDTLFLHADTLRAVFDTVEETSRELMAYHNTRFFRH
                     DIQGVCDSLHYSFADSLITMFRAPILWTEKNQITADTISIVTGKNKVKSVNLHISAFI
                     ISRDSSQTYNQVKGKIMTGYLQDNKLYRIDVNGNAETIYYVREDDNDLIGINKAAGSR
                     MRIYLDNDSKIDRITYFEKPDGNLFPDKDISPADQMLKGFVWYDAVRPTGRNDIFRK"
     gene            18609..20585
                     /gene="gatE"
                     /locus_tag="PKI35_04950"
     CDS             18609..20585
                     /gene="gatE"
                     /locus_tag="PKI35_04950"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HNX43766.1"
                     /translation="MNHSFDARGNYEASREKIGYIPRQKATQADYDRIGFMSGLEVHQ
                     QLLTAHKLFCRCPAGIYQKPDQYDAEVVRHMRPTLSELGEYDGTALMEFKTRKEIIYR
                     LKSVTACTYEVDDTPPFPLDRQALEIAIQISLVSKLNIVGEVHITRKQYLDGSIPTGF
                     QRTAIIGVEGEIPLKNKKVRLIQLSLEEDACREVSDIGHTRVYRTDRLGMPLIETVTY
                     PEMVNPDEVKEACNYIRFLNRSTGKVRTGIGAGREDVNVSCRGGSRVEIKGVAHTRWI
                     PELTHNEGFRQWALLAIRDELLSRIPDIDTWKIRSEKLDYDSIKTSYDPITKARYYKH
                     RLVAINLPQFKGILSHFTQPGKMFADELSERLKVIACIEKPHMTHTEEFEPVISEGEW
                     HKIAKMLEAGENDAQLIIWGPEADIATALETIEERCRMAFKGVPNETRKSFGDGTTIF
                     ERVLPGADRMYPDTDSAPIPLSNEYIDELGKGLPEDVIDRYYRLKEWNVPEDTYTYIF
                     KKNLFPLIERIVSELKLDSAFVGKLFGHSLKWVEGHYKPSAEFDYKVIYALLRFLQGE
                     KLDLSIAAKMLPVVYQYPKMDFESVLTSIRFKRLPKEQILSQIPFLKKKFAEIRHSKS
                     DSVEKNWIMGQIRKASEGNVNLRMLSEEVEKLIS"
     gene            20630..22015
                     /gene="gatD"
                     /locus_tag="PKI35_04955"
     CDS             20630..22015
                     /gene="gatD"
                     /locus_tag="PKI35_04955"
                     /inference="COORDINATES: protein motif:HMM:NF003217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HNX43767.1"
                     /translation="MSDDIFQGYKGAALEVLKKYNVRVWGQATVMTTRGKFMGTVLPR
                     AENDDDKHIVMKIVTGYNVGIDIATVTDMKETGYKKANYKIPEKEFPYTQGLPKVKLI
                     GTGGTIASRLDYRTGAVIPAFSPGELYGAVPELATICNLTTEKIFAVFSENMAPDNYK
                     KLAIAIGKEIEAGVDGIVIGHGTDTLHHTAAALTFMVQNSPVPIVLVGSQRSSDRPSS
                     DAALNLMHACTAAGHGDIAEVMICMFGPTSDEYGFLHRGTRVRKMHSSYRSTFRTIGD
                     TPLATVTRQGVTPIKQDYNHRRKDRNVKILPYFEEKVSIVYYYPNMQPDIIDSLVENG
                     YRGIVIAGTGLGHVNKPLYPAIERAAKAGVAIYMTVQTLWGYVHMFVYDTGRDLMAKG
                     IIPAENMLPEVAYIKLGWALGQTSDLNEVKRLMLTPINGEITPREPYNGYLIYQGGVK
                     EVEDFIRKVHK"
     gene            22039..22569
                     /locus_tag="PKI35_04960"
     CDS             22039..22569
                     /locus_tag="PKI35_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43768.1"
                     /translation="MALIKLVEKEEATGRVAEIFETMTNTMGFIPNAFKVFSPSSHVL
                     DTQFGNLVYFMRHKTLGGKLLAFIRFLVSEEEVCAYCVGMNSGILFQYGVLPDQLAEI
                     RKDPSTAPLEEKEKALLLFVLKVVRDSNSIGQQDVDALKHLGWNDADILDATYHGATQ
                     VGVDKIFNAFKLEMDR"
     gene            complement(22584..22796)
                     /locus_tag="PKI35_04965"
     CDS             complement(22584..22796)
                     /locus_tag="PKI35_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNX43769.1"
                     /translation="MMTEFQLHEHEFIPLDKLLKITGLVESGGEAHLCIDAGEVTVNG
                     DKELRRRRKLHSGDTVVFRGRQVEVR"
     gene            22984..23424
                     /locus_tag="PKI35_04970"
     CDS             22984..23424
                     /locus_tag="PKI35_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HNX43770.1"
                     /translation="MEMKVVEPKVVYTTRLVKSEDLNHHGTLFAGRTAEWFVEAGFIS
                     AAKLVDPQNIVCLKIHGMHFTKPVAAGEIIRFESKIVYAGKSSLFSYIQVFKEGIEVP
                     LVSGFITFIHVDENTKPASHYIEIEPHTAEDLALYEEAKLLKNK"
     gene            complement(23449..24399)
                     /locus_tag="PKI35_04975"
     CDS             complement(23449..24399)
                     /locus_tag="PKI35_04975"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX43771.1"
                     /translation="MKFEKIIPILKGIILHLPGVKTVLKKSTGGTNNSRYCYSVWLRH
                     LAMLAGNNSSEIPSTVAELGPGDSPGTGLAALLSGCKKYHGLDVVRYWDTKRNLQIFD
                     ELTALFKGRAPIPDSAEFPSVRPEPGTFAFPSRILTDALLSESLSDDRLEAIRREICH
                     PADPGNKFIFCQIPWFESDTLDSKSVDFIFSQAVLGCVDDLENTYLAMSRWLKPGGYM
                     SHTIDFKSHGITKSWNGYWTFNDLEWALAKGGRPFLMNRFPYSQHIALHQKFGFKILA
                     MASVRLENHLPTHKLFKRYKPLNTEELTTGGIYVLSRKEP"
     gene            complement(24749..25480)
                     /locus_tag="PKI35_04980"
     CDS             complement(24749..25480)
                     /locus_tag="PKI35_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNX43772.1"
                     /translation="MKIQLIIAASVIIAMTACAPTRTAVNTLTKKEIAEKYQLLFDGK
                     TTSGWRGYEKTVFPKGWEVVDGTLHCIGSSKGEAGSGGDILYEKKFRNFELTLDWKVS
                     EGGNSGIFYLAQEIPGEPVWKSAPEMQILDNERHPDAKLGVNGNRQAGALYDLIPCSV
                     TAVKPAGEWNTVKIMVYKGTVVHWVNGRQVLEYHLWTDDWKKMVLDSKFRDYQWFLNT
                     AQEGYIVLQDHGDDVWFRNIRIKEL"
     gene            complement(25636..27120)
                     /locus_tag="PKI35_04985"
     CDS             complement(25636..27120)
                     /locus_tag="PKI35_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX43773.1"
                     /translation="MEKNEISRRKFIGTTAAATAGITILPNLVFGRNGFLSPSDKLNI
                     AGIGIGGIGATNLKNCSTENIVALCDIDDEYAKGTFETYPGARRFIDYRKMFDEMGKQ
                     IDAVIIATPDHTHAIIAAEAMRRGYHVYLQKPLTHTVSESRLMADMAVRYKVATQMGN
                     QGNSGEGIRKICEWLWDGAIGKVTEAHAWTNRPIWPQGLQRPTEHFDVPSTLNWDLFL
                     GPAPERPYHPAYHPWNWRAFWDFGTGALGDMGCHIFDPIFKALRLGNPSLIYASSSQV
                     NTESAPMASMVHYEFPDRGKYQKLKLVPVKVTWYDGGLLPPRPAELPDGKPMGDWNGG
                     CLFIGTKGKLLCDAYGQNPRLLPEELDKDYKRPEPSLRRIENAMSGGHEQDWIRACKE
                     SPENRTEASSYFGYSGPMNEVVVMGNLAIRLQDLKRKLQWDGEKMMIPNISPDDEIRV
                     VSTDKFTVINGHPHFDTKYDTIKAKPASEEYIRHTYRQGWSLEG"
     gene            complement(27135..28190)
                     /locus_tag="PKI35_04990"
     CDS             complement(27135..28190)
                     /locus_tag="PKI35_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX43774.1"
                     /translation="MERVIQTGIIGYGLSGRFFHAPFLKTNPGFAVTRIAARHSGFAG
                     ELFPDAGIAGDYREIIDDPDIELAVITTPNIYHFPMAKDCLLAGKHVVVEKPFVPTTA
                     EADELIALSRKVQKHIFVYHNRRWDGDFRTVQQILRGRRLGKLLEYEAHFDRYAPLRT
                     RATWRDDPLPASGLLYDLGPHLIDQALLLFGKPEAVFADICSQRPAGKVDDYFHLHLY
                     YSGHKVILKAGVFVKEAGPRYILHGDNGSFVKKGIDPQEELLRKGFLPFSDDWGTDQE
                     ENHGILHFVSDGKDVRTPVPTEAGDYGHFYRNVYDVLAKDSAPAVKPEEARAVIRIIE
                     LAFESNKTKSIIPFTER"
     gene            complement(28200..29048)
                     /locus_tag="PKI35_04995"
     CDS             complement(28200..29048)
                     /locus_tag="PKI35_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNX43775.1"
                     /translation="MPSRRNFLKAASLGAFSLTGLSREVQARPDRKSIGLQLYTVRDY
                     TEKDLTGTLKSLSETGYDFLEAASYSSGKFYGMEPKTLRKLVEGFGMKMNSSHAGFND
                     KDAALAIESHAELGVKYLVLPSMHIDTPDAGAFMKAAARFNNLGEQCRKAGLKFGYHN
                     HSFEFSMTDRGPGYDVLLNNTDPDLVLFQADLFWMKKAGYNPVDYFKKYPGRFELWHV
                     KDMAGTPEKEDTEVGTGIIDFGELFEKKKLSGMKHFYIENDNPHGDSLESVALSYHNL
                     LNLLSK"
     gene            complement(29344..30873)
                     /locus_tag="PKI35_05000"
     CDS             complement(29344..30873)
                     /locus_tag="PKI35_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX43776.1"
                     /translation="MERRDLLKSLAALPVAGAFGFEWFRKRNYEKFLTENIRNELNIS
                     TDTPEYKPVSKDDKLIRIGLIGFGIRGKQLAQAAGFVHPTTIDDWKKGALEDKEDTRY
                     EDFLQQDALNIKISGVCDIFDSYGKMAQEAAANALRQGSGSKLNELPKRYRTYQELLA
                     SPDIDAVIIAAPDHWHGPLTIAAAKAGKHVYCEKPMTWTVPETYEVRRAVKENNIVFQ
                     LGHQGRQNESYMKAKEIIEKGVLGKVNLIEVTTNRNDPNGAWVYPIPPEASPANIDWN
                     QFIGQAPYHDFSLERFFRWRCWWDYSTGLSGDLLTHEYDAMNQILGLGIPHSAASSGG
                     IYFFKDGRTVPDVLQTVFEYPDRDLSLVYSATLASNLSRGRKIMGHDAYMELNNSVTV
                     YADSGSTRYKEKIDKGIIDPDLPIYTYTPGMKNVDAITSPTSQYFAGRGLMYSYRSGR
                     RINTTHLHIREWLDAIRYGTKTSCNIEVAFEEAMTAHMGTTAFKENRNVFWDPVKEII
                     I"
     gene            complement(30875..31372)
                     /locus_tag="PKI35_05005"
     CDS             complement(30875..31372)
                     /locus_tag="PKI35_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HNX43777.1"
                     /translation="MNYSPSQKFWLVLLRIAIGWHFLYEGLAKLVNANWSCVSYLLDS
                     EGFLKNFFINTLAGNPDVLRIVDFMNIWGLILIGIALILGSFERIACLSGIVLLALYY
                     LSHPPFVGLKYAVPSEGSYLVVNKILLELIALAVVYVFPTNREFGLDRFIFGPSKKQT
                     LAKKN"
     gene            complement(31398..32042)
                     /locus_tag="PKI35_05010"
     CDS             complement(31398..32042)
                     /locus_tag="PKI35_05010"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNX43778.1"
                     /translation="MKTLAPLLVALLLILCIPDLYCQKAGNSGRVSGTKHWKMLFNGK
                     TLDGWQIHGTEKWEVKAGMIVASSGPDKGYGYLATEKKYNNFILQLEFKLEADGNSGV
                     FFRSNLNGTDIKGWQVEVAPPGENTGGIYESGGRGWLYEIPDDRENILKPGNWNTMKI
                     ELRGDHIKTWLNGQLMTDLTDAAIGKGSGIIALQVHSGGDVAVKWRKIRILELP"
     gene            complement(32261..32938)
                     /locus_tag="PKI35_05015"
     CDS             complement(32261..32938)
                     /locus_tag="PKI35_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HNX43779.1"
                     /translation="MRPRIIVHNAVSLDLKLTGFDVDMELYYGLAAQWKEDATLAGSA
                     TILAAPGEAPPETEADLIPPVPVPGDRRAVLVIPDSRGRVRIWHYLRQLPYWRDFIAL
                     ISESTPRDYIDYLEKRHIKYLVCGKERVDLKTAVERLGSEFNIKTIRVDSGGALITAL
                     LEAGLVDEISLLVHPVIAGKESSVSLIHELNKGKIHLKLCKAETLDKGLVWLHYVNAS
                     EQGEYSS"
     gene            complement(33295..33945)
                     /locus_tag="PKI35_05020"
     CDS             complement(33295..33945)
                     /locus_tag="PKI35_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNX43780.1"
                     /translation="MNPTFDLKGEHDAMAFIITAMKRISCDIRNDTPVDLFRFGQIIE
                     FLKIYLDHCHNQKEEKVLFPALLGLKIPKINDTILKLLEDHIVARTYVTVIETGFNRF
                     LSGQSLSMQSLPKSIDNFCSLVRDHLYVEEKTIFPLVEKSLDAKQQENIAGGFAYIQD
                     HEVGHEKCEEYYILLSRLYSETKYTTIHDRSGRTGKKSPGKENPSGQPQHTLYVSG"
     gene            complement(33968..35224)
                     /locus_tag="PKI35_05025"
     CDS             complement(33968..35224)
                     /locus_tag="PKI35_05025"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNX43781.1"
                     /translation="MLNDYKKTLCLLFQVHQPYRLRAYGFFDIGAKHDYYDESLNKQI
                     MKRVAYNCYKPANEILLRLIDRYRDAVRITFSLSGTAMDQLSRYAPDVLESFVKLHRT
                     GNVELTGESYAHSLAMLSHPAEYRRQVAEHSNRMESCFGFRPSAFITTNLVYSDEIGN
                     LVTSMGYKTLFTEGARQALGWKSSNHLYSGNSNPDLKLLLRNFQLSDDISLRFSTRDW
                     NEWPLTADKFISWLNAIDQNEEIVNLFMDYGSLGESRLSGTGKLAFLGSLLEKAVKSN
                     RWQLKTISEAAETIHPVANLSIRDVISCADQEHDLSAWLGNELQQEAFNNLYSVSQIM
                     EECEDEELLSDWNNLQTCDHFYYMGTKSTADGAMHRYFSPYSSPFEAFVNYMNVLSDF
                     LIRARDYKTRHDRMVACRSLSGLIIT"
     gene            complement(35601..35990)
                     /locus_tag="PKI35_05030"
     CDS             complement(35601..35990)
                     /locus_tag="PKI35_05030"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HNX43782.1"
                     /translation="MFWFHFLDYFFVVFHLAIVIFNLFGWIWKKTRKANLILLLLTGG
                     SWFGLGIFYGFGYCPFTDWHWQVLDHLGGMPGETSYMQYLYARAFGLHLPAGLVDTST
                     LAAFFAALIISILINIRDFRRKRRSRP"
     gene            complement(36265..38064)
                     /locus_tag="PKI35_05035"
     CDS             complement(36265..38064)
                     /locus_tag="PKI35_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43783.1"
                     /translation="MNLTELDQTEFETEQRKYVYLITAPVPDISTERYLDNHTLLLNS
                     NSLHYLNKNLQLKVNLSLSDDNRRVQARELRSLYLPADTIVTSNHIDNSSDSRELRAK
                     FSLCRNTRGNYLNDQLTLNLTGIDCNGITNYNDERIQQVLENPSYSISNTLKSIKPIG
                     KKLLQIGTDISYVSGNQTLNVTPVIISDSLFLLNNIDSLRQHVSAGKFYTSGYAAMGF
                     TYGKLNIKPELGFVARSTAFNSDLGMFSSADESCQFPDYMNDFACNLFSPYFSTELKI
                     KKLKYTLTTSGKLNYAILSRNSTQDNREKADLLLHEEEVTLVLKPDAFWQIRFNASYS
                     LQPDDISNYYPSYLLANFRELNRVNKPVSVSDKYRFLSYFSYRNQIISFFNSISYSFI
                     TTDYEYSGNRLTSLNGSSVDEYRYMPARTCIHNLNGYSAKTFSALRTTLSLRFNLGFV
                     KGSSLLNGEKAETQNNNITVTPGVMFRFSKWITADYKGSAIWMNNILNDITGNDFYIF
                     KHDLSLLVFPYKNHSLNLTAEYYSYDKKDHFFADFVYQYSVPKPKIDFEMGFRNIFDT
                     DTYTSLYVNSYYSWLYEYSLRPFEIMLTARFTF"
     gene            complement(38119..38892)
                     /locus_tag="PKI35_05040"
     CDS             complement(38119..38892)
                     /locus_tag="PKI35_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43784.1"
                     /translation="MPVKFLMVFAGMMITGMTLFGQVQIAGKVLSENGAGVPNIHVLA
                     YKPGDAVIQAFAISRNDGSFALKVNAKSDSLLIKTSSVQYRNESLLIKNESRELKIML
                     RPESKEIEGVTIKAQPIARYGDTLSYLVNSFAQQNDRSVEDVLKRMPGVTVEPNGRIL
                     YQDIPINNLYVEGMDITGGNYGVVSKNLPQGSVSAVEVFENHQPVRILQDVQFSSQAA
                     LNLKLKQKVATTGTARLGAGITPLLYRFNITPFIFTKKN"
     gene            complement(38917..>39779)
                     /locus_tag="PKI35_05045"
     CDS             complement(38917..>39779)
                     /locus_tag="PKI35_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HNX43785.1"
                     /translation="IKYSMYMSCNKFRIAFAIILTIALHCPNMAVAQLFDLQFTVLDK
                     VKYCIEYSQQSVPDSNNPGNVKNGRMLLFVGDSISLYESRSLYISDTIMRKFQTHEDL
                     QTFASNPNKPSPATRYRIYKNYPPGKLTCIEPLLFDQYKYIEPLNAFDWVMEPDTATI
                     NNYRVQKATCVFGGRNWTAWFCPEIPISDGPYKFNGLPGLIVKMYDSRNHYVLQMESI
                     SIPAGEILIDIEQKIYTESTKEQFFKAKNSMADNAINIVKDKGADDESARTAAQNIAK
                     RNNHIELFRK"
BASE COUNT        10356 a         9043 c         9791 g        10589 t
ORIGIN      
        1 acaacgtggt caatcccgac gacctgattg aaaagtacgg agccgacacc ctgcggcttt
       61 atgaaatgtt ccttggcccg ctggaacagt cgaaaccctg ggataccagc ggagtggaag
      121 gcgtttcgag gtttatcagg aaattctgga ggctcttcca caatgaaaag ctggaattct
      181 cagtttcgga tgaagtcccg acaaaggaag agctgaagat acttcacaaa accatcctga
      241 aaattcagga agatatcgaa cgcttttcat tcaatacggc ggtgagcgcc tttatgatct
      301 gcaccaacga acttaccgac ctcaactgca acaagcgggc ggttcttgcc gaccttgttg
      361 tgctgatctc tccttatgcc ccccatattg ccgaagaatt gtggcatctt ttaggcaaca
      421 ccggcagcgt atgcaatgcg aaattccctg aatacaatgc cgcctttacc gttgacaatt
      481 cattcaccta cccggtatcc ttcaacggaa agacgcgttt catgcttgaa ttgccattgg
      541 atatgccggt tgctgatatc gaaaaagcgg tgcttacagc tccggagtcg cagaaatggc
      601 ttggcggcaa tccgccgcga aaggtcatcg tggtcccccg taagattgtg aatgtggtaa
      661 tctgaatgac cgctgggtaa aagggttatt gcgcctcagc caataccgcc gtgccgtagc
      721 agatcacctc ggttacgccg ctcataatct cagttgattc gtaacgaata ccgatgacgg
      781 catttgcccc cagcacttcg gcatggacca tcatctccat ataagcgtcg gcacgggttt
      841 tctcacacag ttccgtcaga agcgaaatat tgcctcctac cagcgtttgc aaaccggctc
      901 cgatggtgcc aaaaatagat cgggaacgta caatgatgcc ccgtatcaat cccaggtttt
      961 tcacgatagt gtaacccgga atttcgaaag tggttgaagt catctcctgt ttcatggatg
     1021 tagcagttat tgcttttgaa tggtataaat ataccgggtt tccttcgaaa taagtatgcc
     1081 tcggaatttt tactgtttac caagcaggaa atcatttgtt taatttcgca aaaaatcaaa
     1141 aacgatgccc gataaccgta atagtcaata tttcagcgga ttaatgctgg cggtgctatg
     1201 ttatacatgt tggggcatct tcccggttta ctggaaattg ctgaaagaag taccctcgga
     1261 ccagattctt gttcaccgga tcgtatggtc attcatattt cttgggattt tgttggcttt
     1321 cctcagaaac aggcaggtta tcagatacct gaaggatatc cgcattgccg ggcttttgct
     1381 gatgacaggc ctgctggtcg gcagcaactg gggcgtttat atatatgcgg tgaaccatga
     1441 ccgcatcgtg gatacaagcc ttggttatta tatgaatcct atggtgaacg tattgctggg
     1501 agtcattttc ctgaaggagc ggcttagccg gattcagaaa ctggcagtgc tgtttgctgt
     1561 tggtggaatt gtatggctca ccctttatgt gggaaggtta ccctggattt ctgtgtacct
     1621 ggcgctatcg tttggcgcct atgggttact ccgcaagaaa gcaaacctgg aatcgcttcc
     1681 ggccgtattt atcgaaacca tgttgcttac cccgctggcc atcggatacc tgttgtttgc
     1741 cgaatatcag gggactggcg cttttttgca tgggtcctgg ctgatatcgg tattgctcat
     1801 cattggcggg cctgtgaccg ccctcccgtt gtattggttt ggcaaagcag cgacaaagat
     1861 accgctgtcg acgatcggtt ttatccagta cctttctcca accatcgcgt tgctgatagg
     1921 gatcttcgtt taccgcgaac ctttccggat accctatgtc gttgcatttg ccatggtgtg
     1981 gacgggcctg gccctgtata cgatcagcat tttgagggac tatcgcacac ggcggctatc
     2041 gtgaataaca ggcctggaca gcatcaaaag tctgactgtc agacggcagg gctcaggctg
     2101 tttggataca gctccggttg taacccggcc gtctgaatat taccctcttt tttctgacag
     2161 ccgtttctgc tgcggtaagt tcctccgtta ccgggaacca gcagccgata gcctgtacag
     2221 gtcaaattta agtgtattca atggttcacg tctgtatcct gcggctgcag gcagggtgtc
     2281 cttcttttcc aggataaagt aatcagggct ttctaccggc gacggatcaa cattgatatc
     2341 gtagtatcga agcagatata gctgcagggg ccagtttgca tatagtgttc tattaattcc
     2401 cgccgttgaa tgttccggga taacgcttcc aagccggtaa acatcgctca gaacctcctc
     2461 gtcacggcag gttttgttta tctgcatggc agaaaaaacc aatatacctg caaggaaaac
     2521 caacgtaatc aaccggaagg ccctgaaact cccggaagca ggatctgatt tttccgtaaa
     2581 atgaaccatg aaagggactg atatggctgc cagccccagt gtaaaaagcg gaaaaacagg
     2641 aaccaggtaa aaaccttttt gcaccagcgt gaacaacata ggcagcgagc ctgccaatcc
     2701 cataataatg aacagcaggg cctgtttccg gtaattattt ccgggattgt atttacgaaa
     2761 ttttccggta aggatcagcc cgccggcgat aagtagcgcc gggatcagtt cagtaaaaat
     2821 tctccagatt acatcgaacc ggttactggt agttacttcg ctttcaattc ttgaaaatac
     2881 cctttcgaag agccagaatc tcagactttc ggaagcgccg ggaatcaaca aaataagaga
     2941 aaaacctgcc gcgacgatag cgagaaaaat tagccagtaa agcaacattt tccgcaaggt
     3001 gaaatccttg tgcagcgcct tatacaccgg tatgaaagcc agtggaaaca gtccgggcag
     3061 cccttttgac aacactgctg ccacggtaaa aattccggca agcagaagat ttggaagata
     3121 acgccggctt ttttcgacag cccgaagcga aaaatacacg gcagccaggg tgaatattcc
     3181 catggtattt tcctgcatat tgttccggta cgaccagaaa caaacaggcg tgatgatcca
     3241 taaaaggacc ggaaaccatt ccgaattcct tatcttttct tcctttctga atacaaaccg
     3301 ccagatcatg gctatcagcc aggctgttat cagagctgtc aggaagctgt atatgcgctc
     3361 ggtgtacctg ctgtcgccga gagccctgaa aaacaaggat tgaattccga aaaccagggg
     3421 cgggtgttcc aggaacgcat ggttgccctc tttgttccac gtattgctga ggtgcggctg
     3481 ccagaaagtt ccctgcccgt gagccagatt tttcgagacg caggcatact gcaacccgtc
     3541 catgaacatc ccgtctttca gcagcgccgg aagaatgagg ataagtatga tgccgaatgt
     3601 caatactctg aacgggaaat tttggctttt caaacggaaa aaaaattata gataagttta
     3661 ttaaacaaac tgttcgagaa gttctatccg tttccggaca ggcggatgcg ttgcgaagag
     3721 attgtcccac gaaaatccgt gagccgaagg tttgggattt tcgataaaga gctgggccac
     3781 atcgcggctt tcaacagcct caatagcagg atcatttgct attttgcgca acgccgaagc
     3841 cagggcatat ggcttatgcg tcatggcggc tgaaccggca tcggctaaat attcgcggga
     3901 gcgggatacc ccaaaacgaa gaagcatgga aaccaggtag caaatggctg ttaaaaccac
     3961 tgctatcaga atgatcatac ctccgttttt gccgttgctt ttacttcccc tgccgataaa
     4021 gcgaaaactc cggaaagcca tttcggcaag gaaggagaaa atacctacga aaatgatgga
     4081 gataatcagt aaacgcacgt cgcggtttcg gatatgactt aattcgtgag caataacact
     4141 ctccagttct tcatcgtcaa gattttcgat gatgccccgc gataaggaaa cggcgtaggt
     4201 tgaatcgttt attccgcttg caaaggcgtt gagcgagtca tcttctatga catatagccg
     4261 cggcattttc atgccattgg caatgcaaag gttctcaagc aagttgtaaa cccgtttatt
     4321 ttcgcgcctt tcaaggggct ttgacccggt tgccagcctg atcagcgagg attgcgcaaa
     4381 ccaggctgcc aggaaccaga tggccgttcc ggccagtaca tagggaaccg cagcgataaa
     4441 tgcggcattt gcgccgggag ccctgtgccc gctggtgaaa aacaggaaaa tataaaccat
     4501 tgccagcagc aataccggga aggcaatgag caacaggact gaattcaggt tgtttttccg
     4561 gatctggccc tggattccga tatattccat gaaattcaga acttaatggt gggcggttcc
     4621 tccaattgtc cgcgtttttc agggcccgga tcaaacatgg tttcacgttt gaaaccgaaa
     4681 ttcgaggcga tgatattgga cggaaatgtt tcgatgccgg tattgtattc ggcagtggca
     4741 gcattgaaaa aacgcctggc tgctgccagt ttgttctcta tatcgctgag ctcttcctgt
     4801 aactgaagga agttttgatt tgccttgagg tcgggataat tttcaaccgc gatctttaag
     4861 ccttgcaatg cattgccaag cgttgtctct gcctttattt tttcgtcaat agtgccggcc
     4921 tgtatggcgg cagcgcgggc ttcggtgata cgggtgagca gttcccgttc atgggcggca
     4981 taacctttta cggtctcaac aagttgaggc acgaggtcat ggcgttggcg caactggacg
     5041 tcaatatcgg caaaagcatt ttcccgcctg ttgcggagcc gtatgatgcg gttatacagg
     5101 ctgataagcc aaagcacaag aattgcagca atagccagaa ttacaattat tgccatgatg
     5161 attttcgtat taaggtgaat acaaatgtaa taatttccgg gctgatccgg aatcctgcct
     5221 tagccccggt tgcaattcag gtaaatattg tgaggaatgc cggggaaccc gtttagccgg
     5281 ggaattggaa aacagttcac cggaagcatg atcagatcac gtgccggatc atcatgactg
     5341 acagagaata ttgatagcag gatacctctc ccggaaatgt ctccatatcg ggcaaagcca
     5401 ataaatcagg tatgcggttg atgtcccatt tgccaggatt gccacttttt tcgaacttat
     5461 aaattgaaaa tttgtcaata tgttgaagga atcctcagat attgattcag cttatctggg
     5521 aatcaaaaga aaaatattaa ttttgaaccg ggaatgagta tgactttttt tactgaaagt
     5581 gattgattgt tgatgggctt acaggtctgc cgtcccactg cactgcctgc aattgagtat
     5641 taaatattgt tgtttttatc agtttaacgg atactttcca tctgagtggt gggaaattat
     5701 gaggatttta ctatggatac aaaaaccaac atcaacggca taaaatatac agatgcgctt
     5761 tatgcagatg aatatccacc tctggtagcc tattttttaa ttgatacctc cggtgtagtc
     5821 ctggattttg atgcacaaac agccgcgatt ctgaatgttc agggcccttc gttcaaaggc
     5881 gtcaacctgt tattacaggc gggggacaaa agctttgtac attccctgaa cgaagcgtta
     5941 tcagggaaaa caacctatca tagcggtcag atatcgcttg ccggaagaga gccgcgtaaa
     6001 aatttcgcca cctacctgaa accggtttat tatccatccg acaaactgat cggcgttgcc
     6061 ggttttattc actatgccag gccgggcatc gtatcccctt tcgagatggg caggcaggcc
     6121 ggctatctga acctgattgc ggaaaatacg catgacatca tctccttgca ttccttcgat
     6181 ggtaaaatat tgtttttatc accttccttt gagcaattta ccggaatacc ttatgacaag
     6241 gcgatgcagg atagcgctgt atggcccgtt tatcctgacg acaagcaaat tgttcttgat
     6301 gccattgctg ggatgaagga tggctcctat cctgtaaata tcgagtaccg ttatataaaa
     6361 tgcgataaga gtatcggctg ggccgagagt aaattccaga ttattagcaa cagcggcatg
     6421 catgattgtg tagcggcggt tacgcgcgat ataacggcac gaaaggcggc agaggatgcg
     6481 ctgatacgca gcgaaatcaa ataccggaac ctgatgctga acctgccgac cggtgtggtg
     6541 ctccttaacc tgaacggtaa aattatagaa gccaacgatg ctttttttaa aataatcgga
     6601 aagccgtcat cggaactggg taacttgctg tcaattaaag aaatcaatca aaaatacaac
     6661 tgtaatgtct ctgcggaatt caacctttgc atttccgaaa aaaagccggt tgaagggcag
     6721 caggaattgg caacgcctgg caatatcaag cggcatattg tctatagcct ggtgcccctt
     6781 accgatccat cggggaaggt ttcggctgta atgggcaata tccgcgacca taccgacctg
     6841 ataacggccg aagaagaaag ccgtcagcag gttgaattcc tgaacattat catcaatacc
     6901 ttacaggagc ctttctttgt taaagatgag catcacaagt gggtaatgat gaatgacgcc
     6961 aatgttgaga tgatcggcca tccgcgcgac ttcctgatcg ggaaaacaga ttatgatatc
     7021 tttccgaaag aacaggccga tgttttttgg aaaatggatg atctggtcct ggaaaatggc
     7081 gctaacacca acgaagagca gataacctgg agcaccgggg aaataaggga tgcaataaca
     7141 tccaaatatc tctaccacga taaatttgcc ggaaaaaaat tcatcgtcgg cagtatccac
     7201 gacatcaccc acctgaaaaa agttcaggat acgcttaaaa acagcgaaca gaaatatcat
     7261 gacctttttc agaatgccaa tgacctgatc tttaccatcg acctgaaggg aaattttacc
     7321 gatgccaatc aacgggtgat agacatcctg ggaatgccgt tgacgcaagt gcttgcaaca
     7381 tctgttttcg atgttgtaaa aaatattgag atcggtcaat ttaaagagat cctgaaaaaa
     7441 ctgttaaaag ataaatcggt gccggcctat gaagttgagg tgcagtcggc cagcggatcg
     7501 accattgtgc ttgaagtccg cagcaggctg atgtatgata acaatgatac acctatcggc
     7561 atcacgggga tcgggcggga tattacggat caacttactt ataaccaaag gctcaatcaa
     7621 tacaacgatg agctgaagga gctgaataag agcaaggaca agatgttttc catcattgcc
     7681 catgacctga aagatccttt taacagttta ctgggatttt ccgatatcct gcttgaagat
     7741 ttcgattccc tggaccggga tgaaatgcgt gattatatta aaataatcaa caatactgcc
     7801 aagcattccc tgaacctgct cgaaaattta cttacctggt cgagattgct caccgggcgt
     7861 ctgcccttta cccctatgaa gctgatgctt gccgatgaag tggagacggc aacgaccatt
     7921 gtcagcagcc ttgcttaccg aaaacgaata accgtaaaca accgggtgcc gtccgatatg
     7981 gcagtgaatg ccgatcagta catgctgctt tccatccttc ataatttcct gatgaatgcc
     8041 atcaaattca cgaatccggg gggagagata aatattgcgg cagaaatcga aagccagtcc
     8101 tgtggagagg atcctgaagt ttgtatcagc attacagata atggtattgg catgacagcg
     8161 gatgaaatga gtaagttgtt taaaatcaac ttgctgcatt caactacggg cacacagaac
     8221 gagaaaggca ccggcctggg attgttgctc acccgcgaaa tgattgaaag gcatggtggc
     8281 accgtccatg taaaaagtga accgggccgc ggatcgacat tttcatttac ccttccggcc
     8341 gatctttaac gcagcccttc cggtaatgat tgaccggact gccgttttca taagatcgat
     8401 tttcgaaaaa gtatattatg ttccaaaatg gaatttcggg caatttaatg gagaaataat
     8461 taaaggccct gaattattat ttaattgaaa aaaatggcat aaagggaaga caatgaggtg
     8521 attggaaatt gttaataact attttcggca gcatggcata ctttttgact aatacattgc
     8581 gccataatgt taaacaaact attttcaaaa gaccagagtc ggaataatac caaagaaatt
     8641 ttacgaagcg agagagtttg aattgaactg tttgaaaagg ggaataaata gttcgatggc
     8701 aaataaaatg ttgttaacaa atggctgaaa attactacaa catgactatt tttatctcat
     8761 ctactcgcgt atgcagattc tggggaattt aaactcgggg gaatctggca gcatggcacc
     8821 ggatagtgca attagcgatt tctcaggaat agtatcagac tatttttggg aacattacta
     8881 tgatgccgcc gatgatttca ttgctgttat cgataccagt tgccggtttg tcagaatgaa
     8941 cagccttctt tctgagaaaa ttcagccatt tggtatcgaa gcgggaggga tgtgcgaaat
     9001 ctgcagccgc gagagcaagg aattcaagct ccaatgtccg gtctggcagg cgttaaaaac
     9061 cggtaaagaa cattcaggcg aatttgatga ctcccgtttg gggaatattt ttatcagggc
     9121 gttgccattc tttggccctg acaaatcggt ttcaggcgtg ttgatggtag ccagcgacag
     9181 caacaagcgc gaagtttcac caatttctac cggcctgttg ggccgcgatc cccaatccat
     9241 catcaatgca atgccggagg caattctcat ctgcgataac agcgggatga tacttacagc
     9301 ctctaacaaa cttcgcgaac tgtttgtgct ccctgcaaac aaatcgcttg atggcagcaa
     9361 cttcctgtca ttcctgatgc cgggatatat tgaatcttcc ctgacggatt tcctgacggt
     9421 tgcgaatgga acgaaaaatt ttataaaggg gaaatacagg ttaaggcgca ctaccaacga
     9481 aggtttctgg gccgaagtgt tgttttcacc gctgaaggat tcaagaaacg atcagtccgg
     9541 gatgctgatc gaaatccgcg atatttccga cacgctccag gcagaggaat cgctgaacaa
     9601 aaattatata cggcttacca ggcttcataa aacgtttacc ggtttcagtc ccgattcgga
     9661 aaaaaatatc ctgcagctca ttgcccttta cggtgaattg ctgggcgcat cggtatgttc
     9721 ctattaccgg atcgccaacg gacattccca actggttgca ttatgggact ctccgctgat
     9781 taccggcgtg atgcctgata cggcttatgc cgatattgtg aaacaataca gcagcggcag
     9841 ggagaatttc ctggttataa acaaaccttt taacttcagt gatgccggat tcggttcctt
     9901 catcgatgat aacggcatta ccatgattgt tgtgaatccc gttcggaaca acaataaact
     9961 ggtagggctg atatcggctt tctttacgca tcattacacc ctccggaacg aggataaaga
    10021 gttctgcaat ataatagctt cggctacagc cgtagaagaa tcccggatat caacgcaaaa
    10081 agcgcgcgaa gaaagtgaga tgaattaccg cgagctttcc gacaccatat ccgatgccat
    10141 ttacgtgatg gatcccgatg ggaaagtgat tgacgtaaac cttggcgcta tcggcaaata
    10201 tggttacaca cgtgaagaac ttatcggttc aacaccccgg atgatttcag ccgaagggcg
    10261 taatgacctc gaactgttca atgaatatct gaagaaggct tttaaaggtg aaacaattaa
    10321 ttttgaatgg tggggaaagc gcaagaatgg ggaagttttt gccaatgatg tggtgctgaa
    10381 ccggtcccgc tacttcggtc gcgatgttgt gctggccatc ggccgggata tcaccgaacg
    10441 gaagcaggcc gaagaaaaat tacagcaata caatgttgag ctcagggaaa ccaatgccag
    10501 taaagataaa ttcttcagca tccttgccca tgacctgaaa aatccttttc agggattgct
    10561 tgggttcatc gatctgttgg tggaagatat cgacgaactt gaaaagcaac aggtaaagga
    10621 ttatctgcag aatatccgct cagcctccta tcaaacatat tccctccttg aaaatctatt
    10681 ggaatggtcg aggattcaaa ccaagaaaac cccgttcctg cctaccgtgt ttgagctctc
    10741 agccgaaatc gaagagatat tttcgctgct taccggtaac gccatgcgca aggggatcaa
    10801 acttatcagt gatgttccgg ccggcgtatt cgttgaagcc gacagaaata tggcccactc
    10861 catcatccag aacctggttt cgaatgccat caagttctcg aattcatatg gtaaagtgac
    10921 cgttgaaagt tcaattgtaa actcccccaa tccgggaaaa gagcctgttg acgggcgcaa
    10981 gtggctgttg atcagtgtgc tggataacgg cgtcggcatt cccgatgaca ttatggccca
    11041 gttgtttaaa ctggatggcc aggtttcgat ggtcggcacg gccaatgaac cgggaaccgg
    11101 actggggctg atcctgtgta aggaaatggt tgagaaaaac ggggggaaaa tctgggtcaa
    11161 cagcgaatcc ggcaaaggtt cgatcttttc attcaccctg ctgtatcatg agtagaaccg
    11221 ccgtgtttgc cggggcatta cggcgactct gacggcttgc ctttttacag actgtcgcgg
    11281 aagtctttct tcttggtgag tttcaggtcc tgcagcacgg atgatttcac gttaatggca
    11341 aagctccagc ttttttgtgc gccataggga atccagttaa agctcatttc ccagcagtgc
    11401 aggttacgat atatggaaac ctgcgtcagc gaaaagtctt tgttcttaaa atcgtagccg
    11461 ctacgcaatg tcactttcca tttctgcgtg atgctgacgt cgccgttaaa atcgatggtc
    11521 tggtaaacgg tttttttctt ttcgccggta tacctgttgg caagaacgcc ggtgtaacgg
    11581 aaggtgtagt ttatccttaa attccaggga ttgtcccaat caataaactg ttcagggtta
    11641 ttgcggatgt attccatctc gtcagccgtg cctttgcctg tttccgtctt ctttttcatc
    11701 ttgtcggatg acctgaaatc ataaccgaga ctaaggctcc atgctgaatt ttcgaaacgg
    11761 aagagtttac ggtttacctc ccactcaaac ttgttatagg cttgtttggt gaccgagtca
    11821 accgcataag ggctccacga actgctgtaa tttacctgga tctttttaaa taacgtggtg
    11881 cgtcccgaaa tgttcaggtt cgaccatcgc atcgaatctt tagccagatc ataattggtg
    11941 ctgaaattga gattatcgat cagcattacc ttggttgttc cgttgaccgt atctttcttt
    12001 gaccttacct tcatttcaag gttgttcccg agtgcaaagt ttattgagcc ggattttccc
    12061 ctgggcggag agccataggg gctgaaagtg ctttccccga agatggaata taatacttct
    12121 tccccttcag catcctgata agtgcggtaa taacccagtt tgccggtggt gaaatcaggc
    12181 acaaaattaa aacctaccga tggcctgaca acatggcgaa tggcctttac cggcgatttc
    12241 tttccgaact ggtacatgcc gtataaagta gtactgaccg atgaattgaa gctgtaatcg
    12301 cgggcagccc tgaattccga aacggtatcg gtttgaacat gaggggcaat ggtatcgccg
    12361 gctatgtaag tggtatcgct gatataggat ttccggaccg ttttaaaata ccacctttca
    12421 ttataattga tgccatttga ccaggtgaaa tatttcagga ttttgaatga cccctgaacc
    12481 ggaatgctgt gcttcatgcc catcatgaac ctgtcgagcg actggcgggt aaaaagcgct
    12541 gtatcaacgg tgttcagatc gttcctggcg ttcatattgt aagtaacggt gatgctttcg
    12601 taccagcgtg tcggccctgt tgcctgtttc cgtttgaacg gatagaaccg gttcatggta
    12661 agcgctactt cgggaagcga cagcgaaacc gatccgtttg tcgtattctg gctgtgtctg
    12721 gcgctggcag tgatattgcc gatttcacca agcttgcggg aataagaaat actggatgaa
    12781 aaggtgtttg tcaggtaatc gttgaccgac agggggttgt tgtacctgtt gtatttactt
    12841 gaccctgctc gcacgctggc cgaaaatacg ctgttggggt ttgctttgga atcctggctg
    12901 tgcgaccagg taacgaaaaa atccttgctt gtggcataat cagcagaacc tttcacccct
    12961 gtgacattcc tggcatagtt caggttgaag ctgccggaat acttgtaccg cctcttatag
    13021 gtaaatgtcg gcttaacagc ccaactgccg cgtgagtaga tatcgcccaa cagcttcaag
    13081 tcgaaatagt cgttcaggcc gaagtaatat cccccgtttt cgagaaagaa accgcggttg
    13141 gccgattcgc cgtatttggg tatcacaata cccgagctgc gccctttttt gcttggaaaa
    13201 ataccgaaag gcaggacaag aggcagcgga acatcttcaa tactcagata ggcggggccc
    13261 gtaacaatct tatcgtcagg gataatacgg gctttcgagt atttaatggc aaaatgaggg
    13321 tgatcggtgt tttcgcaggt cgtataccaa ccctgttttg aattggtgga gccatcggct
    13381 agttttttca ccacgttgct ttgcaggtag ccttctcctt cacgcgttac gatattctgg
    13441 ataatgcctt ttttcgtttc aaaattgtaa gtcagcgttt tggatgtaaa ggaaaggttg
    13501 ccctgagtaa ataccggggt tcccacggca gcccccgttg aatcggtggt gccttcggcg
    13561 taaatggaac tgttgttgaa attgataatg aatttgtacg catctatttt aatgtcaccg
    13621 tactcgatat gcgcattgcc gattaaatag gcgagtttct gtttgaggtc caaggtaatg
    13681 ctgtcggatg aagaatacct gatgatgtcc tcaatcatct gtggcttttt cttcagtgta
    13741 tctggcctgg aaagcgtatc ctgccggctt agggtatcag gcgggaggct gaaagaagag
    13801 tcatgggtaa cggccagcgt atccggattg ctgccggaga aaaaagatcc ggcttctgca
    13861 acggtcgtta tcatgataaa accaatggtt atcaacagtg acaggaaccg atgcattttt
    13921 tgtaatttag gcacgtgctg gatttaacaa tgtattttgg tgctatcttt gttacatatc
    13981 cgcaaaatag tttgtccctg aatcatcaga taattacatt taattaaccg acatgtatga
    14041 ttcaaagaaa tttaaacgtt gatatgtacg gatatagttc gcaattgcgc cgcaaaacta
    14101 attaaaataa acggatgaca gaaaaacaca gatcaccggc ctttcgtata gtgctgtttt
    14161 tgcttttggc cagtgcggtg gcgtatggac aaaaggtgag tactgttgtg attgacgcag
    14221 gtcacggagg gcatgatacc ggggcgctcg gtaaaaattc gcgcgaaaag gatgttgcac
    14281 tgtccatcgc actcgaacta agggattaca tcaggcaaaa ccttcccggg gttaaagttg
    14341 tccttacccg tgataaggat gttttcgtgg aattgtaccg tcgtgcaaaa attgccaacg
    14401 agagcaatgc caatttattt atatccatac attgcaattc aacaaaaaca caatcggttc
    14461 aaggtactga gacgtttgtg atggggctgg ataaaaccgg cgcaaacctt accgtggcaa
    14521 aagccgaaaa tgctgcaatc cttcttgaag atgattatgt ttcgcggtac gacggcttcg
    14581 atcccggctc gccggaaggc aatatttttt tcagtatgat gcagaatgcc tttttgaaca
    14641 acagcctgga ttttgccggg cgggttcagg ccaacttccg cgacagggtt aaaatgaacg
    14701 atcgtggtgt aaagcaggcc ggtttcctgg tgttgtacaa aaccgctatg ccttcagtac
    14761 ttatcgaatg cggctttctg agcaattcct cggatgaaaa attcctgctt tcacctaaag
    14821 gccaagccca gattgcttat gccatataca aggcgctggc cgattataaa aacggcgttg
    14881 agaaaaacgg cggtccgctg ataaccgccc gggattctac cccggttcaa cggactgtaa
    14941 aagaaaaaga ggttatgccg gtgattgccg aagaggtgaa aactacggct cctgccgttg
    15001 ccggcaatga caatgccgga accccggttc ctgccggagc agtgaggtac agggtgcaat
    15061 ttgccttatc gaaaacgcag atgccgctgg attcccctga atttaaaggg attgaaaatg
    15121 taaaaagcta ctttcacaac ggaatgtaca aatataccgc cggtgacaaa cccagcctcg
    15181 aagaagctat gcaactacgc cgctcgatgg tcgaaaaggg attcaaagat gcttttgtgg
    15241 ttcccttttt agatgaaaac cggattacca atgaggaggc aaagcgatta acaggtaatt
    15301 tgaagtaatt ttgtgattca tttcaatcag gaacatgaaa atacggcgcg atgtaaaaat
    15361 tggggtattc agtgcaagcg ttatcctgct tttcttttta gggatcaatt tcatcaaggg
    15421 caaggatatg tttcgcaagc accggaagtt ttatgcgcgt tatgaacacg tggcagggct
    15481 ttcgtcggcg gcttcggttt cggtgaacgg ccttgatatc ggtaaagtga gcgatgtgca
    15541 ttttgccggc gatgcgtcga acaatgtgat cgttgaaatg agtatttata ataacattca
    15601 aattccggag aattcggttg cccgcatttt tacccccgac atcctgggca ccaggtcaat
    15661 agaaatcgta atcggcgatt cgcccataat ggccgaaaac ggcgacaccc tgatttcgga
    15721 gatgcaggct accctttcgg aagaggttag tcagcaggta gctcctttaa aacgtaaagc
    15781 cgagggcctg atgggttcta tcgataccat gatcacggta gttcgcagcg tgctgaatct
    15841 cgaaacccgc gaaaatctga acgcttcatt tcagcatatc cgtcagacca tttttgccct
    15901 ggaacatact acttacaacc tcgataccct tgtttacggc cagcgcacac gtctcgaaag
    15961 gatattttac aatgtagagt ccatttcgga aaaccttcgg cagaataatg ataagataac
    16021 caacatcatc gataatttct cagccatcag cgatacggtt gccagggcaa acctgtcgaa
    16081 aacactgctt gatctgcaat attccgtgac aagcgtttcg atggccatgg aaaaaatcaa
    16141 caatagcgaa ggttcaatgg ggctgttgct gaatgacaaa aagctttaca atgacctgga
    16201 atcggcgggt aaagagctca acagcctggt taccgacatt aaactgaacc cgcaccgtta
    16261 cctgaatttc tcagtgtttc cccccggcgg gaaaaggatg aagtataaag aacccgaagg
    16321 caaataaggc ttcggcagta ttccggtata ttatttattc aaatccaaag tcgagataat
    16381 attacatatc tgtggaaatt aatcagataa cagaaaaggt aatcgcatgc gccattgaag
    16441 tccataagcg acttggcccg ggattattag aatcagttta tgaagaatgt ttatcatatg
    16501 agttgatgag cgccggatta gcaattgaga ggcaagttcc ggtaccggta gtctataaag
    16561 agataaaatt ggaatgtggt tacagaatag atattctggt tgaaagaact gtactaatag
    16621 agttaaaagc aatcgaagca tttgctcctg ttcatgaagc acaaattcta acttatatga
    16681 aatttgcaaa aaaatctatc ggattgctta ttaattttaa tgtcacttta cttaaagatg
    16741 ggattaaacg gtatagatat taactctttg gttctctgtg aaatcctcag tgaagctctg
    16801 tgtaacaaaa aaaattagca tagagagact cagaaaatcc actgagttta acgtagaagg
    16861 ttcaaccctt gatctgcatt atttcctgaa aatatcattc cggccggttg gcctgacagc
    16921 gtcgtaccaa acgaatcctt tcaacatctg atcggcaggg ctgatgtctt tgtcggggaa
    16981 caggttcccg tcaggctttt caaaataagt gatgcggtca attttactgt cgttgtccag
    17041 gtatattctc atgcggctgc cggccgcttt gttgatcccg atcaggtcgt tgtcatcttc
    17101 ccttacgtaa taaatggttt cggcattccc gttcacgtcg atgcggtaca gcttgttgtc
    17161 ctgaaggtaa ccggtcatga tctttccttt cacctggtta taggtctggc ttgagtcgcg
    17221 cgagattata aatgccgaaa tatgcaggtt taccgacttt actttatttt tcccggtaac
    17281 aatgctgatg gtatcggctg ttatctggtt cttttccgtc cacagtatgg gggccctgaa
    17341 catggtgatc agtgaatcgg caaagctgta atggagagaa tcgcagacgc cctggatatc
    17401 gtggcggaaa aaacgggtgt tatgataggc catcagttcg cgggatgttt cttccacggt
    17461 atcgaaaaca gccctaagcg tatcggcatg aaggaaaagg gtgtcttttt catcgcccag
    17521 tctggcaaca gcgctgtcgg tgaccatggc aaacccctgt ttccggaaat attcggcata
    17581 attgccggtt actatcaggt tctgtgcggt atcggtaagg ataatatgtt gcacggcttt
    17641 gccattcttc cccacttcgt tataatacag gctgtcgccg gtaagccgcc tgccttcgtc
    17701 aaccagcagc gcattcacgc tgaagcacga ttgtcctgtg atggtccggt attctccgtt
    17761 ttcacaatag aggtaactct ctttcccgcg aatgatggtg ggcccttcaa aatagcaaat
    17821 ccgcgactgc gtgttgtact tcatcgtatc gcagttgatg gtataatccg gatttttcat
    17881 caccacatct tccttgaaaa acatcatctt ggtatcggtg tggtaatccc cctttttact
    17941 tgtgagggta ttatttttat tgacgacttt cccgcccgag ttgtaatacg cgatttgcga
    18001 gttcctgtcg aaaatcaggt agttggtagt cagggtcgtt tggttatcca ccagtttcac
    18061 atcgtcggat atcctcgcga tgcgggtgtt cccgtcataa tgtaaaaatt tgccgtacag
    18121 gttcagcgta tcgctcgatt taatgcggac atgcccgtaa cagtccacgt tgttggtttt
    18181 ttcatcaagc caggcgctgt cgcagtaaag atatgccccg gcatgttcga aaaccggatg
    18241 cccgattgcc ctgacgatat tttcaccgat cagtttatta cctgccaatg ctcctgcgcc
    18301 tacaatccgg atacgaaagg tcccctgggc atggagcatt ccggcagcca gcattattgt
    18361 tgcggccagc agaaatcgta tcgggaattt tagtgatgac atttgtagaa ctattcaggt
    18421 atccggtgtg caaaggtaaa gttaattggc aaattacaga ttcgaagctg tggggtgatt
    18481 gtgctatgct gattattgca tacgtatcag gatcagaaat tcagggaaaa cgttttttct
    18541 ttttatatta aagcctcatg tatagcggta attcactaat tttgcggctc tcaaactcta
    18601 caatcttgat gaatcattct ttcgacgccc gcggcaatta tgaagcatcc cgcgaaaaaa
    18661 tcggatacat tccccgacaa aaagcaaccc aggctgatta cgaccgcata ggtttcatgt
    18721 cagggcttga agtacatcag cagctcctta ccgctcataa acttttctgc cgctgcccgg
    18781 ccggtatcta tcagaaaccc gaccagtacg atgccgaagt tgtccgccac atgcgcccta
    18841 ccctcagcga gttgggtgag tatgacggaa ctgctctcat ggaatttaaa acgcgcaagg
    18901 aaatcatata tcgcctgaag agcgttactg cctgcaccta tgaggtcgac gatacaccgc
    18961 catttccact cgaccgccag gcgctggaaa ttgccatcca gatatcgctg gtgtcaaagc
    19021 tcaatattgt cggcgaagtt cacattacgc gtaaacagta cctcgacggc agtatcccta
    19081 cgggatttca gaggacggcc atcattgggg tggagggaga gattcccctt aaaaataaaa
    19141 aggtccgcct gatccagctt tcgctcgaag aagatgcatg ccgggaagtt tcagatatcg
    19201 ggcatacccg cgtatacaga accgacaggc tgggcatgcc gctgattgaa actgtgacgt
    19261 accccgaaat ggtaaacccc gatgaagtaa aagaagcctg caactatatc aggttcctta
    19321 accgcagtac gggaaaagtc cggacaggca tcggcgccgg aagggaggat gtgaatgtga
    19381 gctgccgcgg cggttcgcgc gttgaaatca aaggggttgc gcatacccgt tggattccag
    19441 aacttacgca taacgagggt ttcaggcagt gggccttgct cgctatccgc gatgaattgc
    19501 tcagccgtat acccgatatc gacacatgga agatcaggtc tgaaaaactt gactatgatt
    19561 cgatcaagac cagctatgat cccatcacca aggcccgtta ttataaacac aggctcgtag
    19621 ccattaacct gccgcagttc aaggggattt tatcccactt tacgcaaccc ggaaaaatgt
    19681 ttgccgacga actgagtgaa cggctcaaag taattgcctg catcgaaaaa ccgcatatga
    19741 cgcacaccga agagtttgaa ccggtgattt ccgaagggga atggcataag attgccaaga
    19801 tgctcgaagc cggtgaaaac gatgcccagc ttatcatctg gggccccgaa gccgatatag
    19861 ctaccgccct cgaaactatt gaagagcgtt gcaggatggc ttttaaaggc gttcccaacg
    19921 aaacgcgtaa atcgttcgga gatggaacta ccatttttga acgggttttg ccgggagccg
    19981 accgcatgta tcccgatacc gactcggccc ccattccatt aagcaatgag tacatcgatg
    20041 aacttgggaa aggccttccc gaggacgtga tcgaccgtta ttacaggctt aaagaatgga
    20101 atgtccctga agatacttac acctatatct tcaagaaaaa cctgttcccg ctaattgagc
    20161 gtattgtaag cgaactgaaa ctggattctg cctttgtagg gaaacttttc gggcattcgt
    20221 taaaatgggt ggaagggcat tataaaccct cagccgagtt cgattacaag gttatttatg
    20281 ctttgctgcg tttccttcag ggtgagaaac tcgacctgtc cattgccgcc aagatgctcc
    20341 cggttgtata tcaatacccg aaaatggatt tcgaatcagt gttgaccagc atcaggttta
    20401 aacgtcttcc caaagaacaa atactatcgc agattccttt cttaaagaaa aagtttgctg
    20461 aaataagaca ttcgaaaagc gacagcgttg agaaaaactg gatcatgggt cagatacgga
    20521 aagcttcgga agggaatgtc aacctgagga tgctgtccga agaagttgaa aagctgatca
    20581 gctaacaaat aaactactat cattactaac agaaaaataa ttcgcaggaa tgagcgacga
    20641 tatatttcag ggatacaaag gggctgctct tgaggtattg aaaaaataca acgtaagggt
    20701 atgggggcag gctaccgtaa tgacaacccg cggtaaattt atgggcacag tgcttccccg
    20761 cgccgaaaac gacgacgaca agcatattgt catgaaaata gttaccggtt acaatgtggg
    20821 tattgatatt gccactgtta ccgatatgaa agaaacgggt tacaagaaag ccaattacaa
    20881 gattccggaa aaggaatttc cctatacgca aggacttcca aaggtaaaac tgatcggtac
    20941 aggaggtacc attgcttcac gcctcgatta ccgtacaggc gctgtgattc cggctttctc
    21001 gccgggcgaa ttatatggcg ctgtccctga actggcaacc atctgcaacc tgactaccga
    21061 gaagattttt gctgttttca gcgaaaacat ggctcccgat aattataaaa aacttgccat
    21121 tgccattggc aaggagatag aagccggggt tgacgggatt gtcatcgggc acggaaccga
    21181 cacattgcac catacagccg ctgcattgac gttcatggtg cagaattccc ccgttcctat
    21241 tgtgctggtt ggttcgcagc gttcgagcga ccgtcccagc agcgatgcgg cgctcaatct
    21301 gatgcatgcc tgtacggccg caggtcacgg cgatatcgcc gaagttatga tctgtatgtt
    21361 tggccctacc agcgatgaat atggattcct gcaccggggg acacgcgttc gtaaaatgca
    21421 ttcctcctac cgcagtactt tccgcacaat cggggatacg ccgctggcta ccgtaacgcg
    21481 gcagggtgtt acgcctatca aacaggatta caaccacagg cgtaaagaca gaaatgtgaa
    21541 gatacttccc tattttgagg aaaaagtgtc catcgtatat tattacccca atatgcagcc
    21601 cgatatcatc gattcgctgg ttgaaaacgg ttatcgcggt atcgtgatcg ccggtacagg
    21661 gttaggccac gttaacaagc cgttgtaccc ggctattgaa agggctgcca aagccggcgt
    21721 tgccatttat atgacggtgc aaaccctgtg gggatatgtc cacatgttcg tttacgatac
    21781 gggccgcgac ctgatggcca aagggatcat cccggccgaa aatatgcttc ccgaagtggc
    21841 ttatatcaag ttaggatggg cattgggaca aacgtccgac ctgaatgaag tgaaacgcct
    21901 catgcttact cccattaacg gtgaaataac tccccgcgaa ccttacaacg gttacctcat
    21961 ttaccaggga ggtgttaagg aagtggaaga ttttatcagg aaagtacata aataataccg
    22021 gtttaaaaaa attccatcat ggcactgatt aaacttgttg aaaaggaaga agccacaggt
    22081 cgggtggccg aaattttcga aaccatgacc aataccatgg gctttattcc caatgcattc
    22141 aaggttttca gcccgagctc ccatgtactg gatacgcaat tcgggaacct cgtttatttt
    22201 atgaggcaca aaacactggg gggtaaatta ctggccttta tccggttttt ggtttcggaa
    22261 gaggaagttt gcgcctattg cgtagggatg aattccggga tcctgtttca atacggcgta
    22321 ttgcccgatc aattggctga aatcaggaag gacccttcaa cagccccgtt ggaagaaaag
    22381 gaaaaagctt tgctcctttt cgtgcttaag gtggtcaggg attccaattc aatagggcag
    22441 caggatgtgg atgcgctgaa acatctgggc tggaacgatg ccgacatcct ggacgctacc
    22501 tatcatggcg ccacgcaggt aggggtggat aaaatattca atgccttcaa gctggaaatg
    22561 gaccgctgag aaggtttcgt gttttaccgg acttcgactt gtcgccccct gaaaactacc
    22621 gtgtcgccgc tgtgaagttt cctgcgccgg cgtaattcct tatctccgtt taccgtcact
    22681 tcgccggcat ctatgcataa gtgcgcttcg ccgccgcttt caaccaggcc ggtgattttc
    22741 agcagtttgt cgagcggaat aaattcatgt tcgtggagtt ggaattcagt catcatgcga
    22801 tgagtattaa aatgaggggc agccccggat aaaaggatct gtattgagcc ggtcgcctta
    22861 gaacagggta aagataaacc tgtttttgct ttaatccgta aacggcaccc ggattccccg
    22921 ataaaccgat tgtattaacc agtatctttg ccgcctttat taccggaaac aaatacgaaa
    22981 gaaatggaaa tgaaggttgt ggagccgaaa gttgtttata caaccaggct cgtgaaaagc
    23041 gaagatttga accatcacgg tactttattt gccggcagga ctgccgaatg gtttgttgaa
    23101 gccggcttta tctcagccgc caagctggta gatccccaaa acatcgtctg cctgaaaatt
    23161 cacggcatgc atttcaccaa accggtggca gccggagaga tcatccggtt tgaatcgaaa
    23221 attgtatacg ccggtaaaag cagccttttt tcgtatattc aggtgtttaa agaaggtatt
    23281 gaagttcccc tggtaagcgg ctttattacc tttatccacg tggacgaaaa caccaagccg
    23341 gcctcgcatt atattgaaat agagcctcat acagcggaag atcttgcatt gtatgaagag
    23401 gcgaagttgc tgaaaaataa atagcaggcc ggttccctga ggttacaatc acggctcctt
    23461 tctcgataaa acataaatcc cgccggtggt gagctcttcg gtgttaaggg gcttgtatct
    23521 tttaaagagt ttatgggtgg gtaaatggtt ttcaagcctg acagatgcca tggcaagaat
    23581 tttgaaaccg aatttttgat gcagcgcaat atgctgcgaa taagggaacc tgttcatcag
    23641 gaatggcctg ccgccttttg ccagagccca ctccaggtcg ttgaaagtcc agtagccatt
    23701 ccagcttttt gtaatgccgt gcgacttgaa atcaatggta tgcgacatat acccgcccgg
    23761 tttaagccac ctgctcatcg ccagataagt gttttccagg tcatcgacac agcccagcac
    23821 ggcctgggaa aagatgaaat caacggattt gctatccagc gtatcagatt caaaccaggg
    23881 aatttggcaa aaaatgaact tgttcccggg atcagcagga tggcatattt cacgtctgat
    23941 cgcttcaagc cgatcatccg acaaggattc gctgagtaat gcgtcagtaa ggatgcggga
    24001 agggaatgca aaggtgccgg gttcggggcg aacggaggga aattcagcgc tgtcggggat
    24061 aggcgccctg cctttaaaaa gagccgtcag ttcatcaaaa atctgaaggt tcctttttgt
    24121 atcccagtac cttacaacat ccagaccgtg gtacttttta cagcctgaca gaagggctgc
    24181 caatcctgta ccgggcgaat cacccggccc aagttctgcg acggttgaag ggatttcgct
    24241 gctgttgttg ccggcgagca tggccagatg tcttaaccaa accgaataac aataccggct
    24301 gttgttcgtt cccccggtcg atttttttaa tacagtttta accccgggaa gatgaagaat
    24361 gatgcctttc agaattggta ttattttttc aaatttcata aacactccat cctggtttgt
    24421 ttcttgtatg aaacaatcct gcacttataa cagtatatga accggaaaac tgccggggcc
    24481 aaaacactct tgaaaaaacg gacattaagc gcctcttgac ttccatttta atgacgggct
    24541 aaatataaaa attatttttc agcaacaaaa ataattcccg ttttgaaacc ggcaggaggc
    24601 ccacgccggc aaaaacagga ggctgtcgct gaaagcagga ccggtttttc caacgtcctg
    24661 ccgattcatg acagactctt gtctgatttc aggatatccg ggttcccggg cagctatgcc
    24721 cgttcggtta aaccatccgg attaacaatt acagttcctt gatcctgata ttcctgaacc
    24781 atacgtcgtc gccatgatcc tgaagaacaa tgtagccttc ctgagcagta ttgaggaacc
    24841 attgatagtc cctgaatttg gaatccagca ccattttctt ccagtcgtcg gtccagaggt
    24901 gatattccag cacctgcctg ccgtttaccc aatgaaccac ggtgcctttg taaaccatga
    24961 tttttactgt gttccattcg ccggcaggtt tcactgcggt gacggaacag gggatgaggt
    25021 cgtacaaggc gccggcttgg cggttgccgt ttacgcccag tttggcgtcg ggatgacgtt
    25081 cgttgtccag aatctgcatt tccggggcgc ttttccaaac cggttcgccg gggatttcct
    25141 gtgccaggta gaagatgccg ctattgccgc cttcggatac tttccaatcg agcgtaagtt
    25201 cgaaatttct gaactttttt tcatacagga tatcgccgcc gctaccggct tcgcctttcg
    25261 acgatccgat gcagtgaagg gttccgtcca ctacttccca tcctttgggg aaaactgttt
    25321 tttcgtaccc gcgccatccg gatgttgtct taccatcgaa aagcagttga tatttttctg
    25381 ctatttcctt tttggtgagg gtattaaccg ccgtcctggt cggcgcacat gcggtcatgg
    25441 caataattac cgaagccgca ataataagtt gtattttcat agatgtattt atttgtttgt
    25501 tcttatcagg gatatgcttc cgggttgcag gttgaaggaa atcggtacac aggttttcag
    25561 gttgccgcca taaaacagga tctgatagtt gcttccctct tcccttgctg cccgcaggct
    25621 tgcgccggta aggctttatc cttccaaact ccatccctgc cggtaagtat gtctgatata
    25681 ctcttcggaa gcaggtttgg ctttgatggt atcgtatttg gtatcgaagt ggggatggcc
    25741 gttgatgacc gtaaacttgt cggtggaaac caccctgatt tcatcgtcgg ggctgatatt
    25801 gggaatcatc attttttcac cgtcccattg aagtttgcgt ttcaggtcct gaagcctgat
    25861 agcgaggttg cccatcacta ccacttcatt catcgggcct gaatagccga aatacgaact
    25921 ggcttcggtg cggttttcgg gtgattcctt gcaggcgcgg atccagtcct gttcgtgtcc
    25981 gccgctcatg gcattctcaa tccggcgcag ggaaggttcg ggacgtttgt aatccttgtc
    26041 gagttcttcg ggcagcaacc ggggattctg gccataagca tcgcagagca gttttccctt
    26101 cgtaccgata aacaggcagc caccgttcca gtcgcccata ggctttccgt cgggcagttc
    26161 tgccggacgt ggcggcaata atccgccgtc gtaccaggtg acttttacgg gaaccagttt
    26221 cagtttttga tatttgccgc ggtcggggaa ttcataatga accatcgaag ccatcggggc
    26281 gctttcggta ttgacctgcg aggatgatgc ataaatgagg ctcggatttc ccagacgcag
    26341 cgctttgaat atgggatcga agatatggca gcccatatcg cccagcgcac cggttccgaa
    26401 atcccagaaa gctctccagt tccagggatg gtaggcgggg tggtaaggac gttcaggagc
    26461 aggcccaagg aatagatccc agtttagcgt tgaaggaaca tcaaagtgtt cggttggacg
    26521 ctgtaacccc tgcggccaga tcggacgatt ggtccaggca tgcgcttcgg taactttgcc
    26581 gatggcgccg tcccacagcc actcacaaat cttacggata ccttcgccgg agttgccctg
    26641 attccccatc tgggttgcca ctttgtacct gacggccatg tcggccatca gccgcgattc
    26701 agaaacggta tgagtgagcg gtttctgtaa ataaacatga tatccgcggc gcatcgcttc
    26761 ggcggctata atggcgtgtg tatggtcggg agtggcgatg atcacggcat cgatctgttt
    26821 acccatttca tcgaacatct tacggtaatc gatgaaacgc ctggccccgg gatatgtttc
    26881 gaaagtgccc tttgcatatt catcgtcgat atcgcaaagt gcaacaatgt tttcggtact
    26941 gcagtttttc aggttagtgg cgccgatgcc gccgatgccg ataccggcaa tattgagctt
    27001 gtcgctggga gacaggaaac cgttccgccc gaaaacgaga ttcggcagaa tggtaatacc
    27061 ggccgtggca gcagccgttg taccgataaa tttacgacgg cttatttcat ttttttccat
    27121 gatgaggatt tttgttaacg ttcggtgaat ggtataattg attttgtttt attactttcg
    27181 aatgcgagct ctatgatccg tattacggct cgggcttctt ccggcttcac ggccggcgcc
    27241 gaatcttttg ccagcacatc ataaacgttc cggtaaaaat gcccgtagtc gcccgcttcg
    27301 gtcggtacag gggtgcgcac gtcttttcca tcgctgacga aatgcaggat gccatgattt
    27361 tcttcctgat ctgttcccca gtcatcgcta aaaggcagga aacctttccg gagcagttct
    27421 tcctggggat caatgccttt ttttacaaat gagccgttat cgccatggag gatataccgg
    27481 ggaccggctt cttttacgaa aaccccggct ttcagaataa ccttatggcc gctgtaatac
    27541 agatggagat ggaaatagtc gtcaactttg ccggcgggac gttgtgaaca gatgtcggca
    27601 aagacagctt caggttttcc gaagagcagc aatgcctggt cgatcaggtg cggcccgagg
    27661 tcgtacagca ggcctgaagc cggcaggggg tcatcgcgcc aggttgcccg ggtgcgtaag
    27721 ggcgcatacc ggtcgaaatg agcttcatat tccagcagct tgcctaaacg acgccccctt
    27781 aagatttgct gaactgttct gaaatctcca tcccagcggc ggttgtgata cacaaaaata
    27841 tgcttctgaa cttttcggga aagagcgatc agttcatccg cctccgccgt tgtgggaacg
    27901 aatggctttt cgaccactac gtgtttgccg gccagaagac aatcctttgc catcgggaaa
    27961 tggtaaatat tcggcgtagt gatcactgcc agttcaatat caggatcatc aatgatttcc
    28021 cgataatcgc cggcgattcc ggcatcaggg aaaagttccc cggcaaaacc ggaatgccgc
    28081 gccgcaatcc tggtgacggc aaaaccggga tttgtcttca ggaaaggggc atggaaaaac
    28141 ctgccggata aaccgtagcc aataatgccg gtttgaatta ccctttccaa ggtcgatggc
    28201 tatttggata acaaattaag aagattatga taactcaggg cgacgctttc cagcgaatcg
    28261 ccgtgaggat tgtcgttttc gatgtagaaa tgtttcatgc cggacaattt tttcttctca
    28321 aagagttcac cgaagtcgat aatgccggtc ccgacttctg tatcctcttt ttcaggtgta
    28381 ccggccatat ctttaacatg ccacagctcg aaacgtcccg ggtatttttt aaaatagtca
    28441 acagggttat aaccggcttt tttcatccag aacaagtcgg cctggaaaag aaccagatcg
    28501 ggatcggtat tgttgaggag gacatcataa cccggtccgc ggtcggtcat cgaaaattca
    28561 aaagaatggt tgtgataacc gaatttaagc ccggccttgc ggcattgttc ccctagattg
    28621 ttaaaccttg cggcagcctt catgaaggcg ccggcgtcgg gcgtatcaat atgcatggaa
    28681 ggcagcacga ggtatttcac accaagttcg gcatgtgatt cgatggccag ggctgcgtct
    28741 ttatcgttga atccggcatg cgaactgttc attttcatgc cgaagccttc caccagcttt
    28801 cgcagggttt tgggttccat cccgtaaaat tttccgctgc tgtaacttgc agcttccaga
    28861 aaatcatatc cggtttcgga aaggcttttc agggttccgg taaggtcttt ctctgtatag
    28921 tcgcgtacag tatagagttg aaggccgatg cttttacggt cgggacgagc ctggacttcc
    28981 cggcttaaac cggtcagcga aaatgcgccc agtgaggctg ctttcaggaa attacgtcgt
    29041 gagggcatga gtttatatgt ttcagagttt cagagtttca gagtttcagg cctgcctgcc
    29101 cggcaaggtt gtgtgtaagg tggtaggcct gcctgcccgg caaggttgtg tgtaaggtgg
    29161 taggcctgcc tgcctgcaaa ggactgtgag cgaggcggca gggtttcaga cctgcctgcc
    29221 tgggagaggt tgcgggtgag ggggggaggg tttcagagtt acagagttac agagtttcag
    29281 acctgccggc atcatagccg ggtgtattgt ttgatggttc ttatttgatg ggttgatgat
    29341 atatcagatg atgatttctt tcaccggatc ccagaataca ttgcggtttt ccttgaaagc
    29401 cgtggtgccc atatgggcgg tcatagcttc ttcgaaggca acctcgatgt tacagcttgt
    29461 ttttgttccg taacggatgg catcgagcca ttcccggata tggagatgag tggtgttgat
    29521 gcggcggccg ctgcggtatg aatacatgag gccacggcct gcaaagtact gtgaagtagg
    29581 ggatgtgatg gcatcgacat ttttcatgcc gggggtataa gtgtaaatgg gaagatcagg
    29641 atcgatgatc cctttgtcga tcttttcttt gtaacgggtt gacccactgt cggcataaac
    29701 cgtcacagag ttgttgagtt ccatataggc atcatgtccc ataatcttcc tcccgcgcga
    29761 gaggttgctg gccagtgtgg cgctgtaaac aagggaaagg tcgcggtcgg ggtattcaaa
    29821 tacagtttga agaacgtcgg gtacggtcct gccgtcctta aagaagtata tcccgccgct
    29881 tgatgcagcc gaatggggga tcccgagtcc taatatctgg ttcatggcat catactcgtg
    29941 ggtcagcagg tcgcccgaca gcccggtgct gtaatcccac cagcagcgcc agcggaaaaa
    30001 ccgttcaagg ctgaaatcat ggtatggggc ctggccaatg aactggttcc agtcgatgtt
    30061 ggccggagaa gcttcgggag ggatcgggta tacccaagcg ccgttgggat cgttgcggtt
    30121 ggtggttact tcgatgaggt ttacctttcc gaggacgcct ttttcgatga tctccttagc
    30181 tttcatataa ctttcgtttt gccgtccctg gtggcccagt tggaaaacga tgttattttc
    30241 tttcacagcc ctgcggactt cataagtttc cggaaccgtc caggtcatcg gcttttcgca
    30301 ataaacgtgt ttgccggcct tagctgcagc gatggtaagc ggtccgtgcc aatggtcggg
    30361 ggcggcgata atcacggcgt caatgtcggg tgaagccagc agttcctggt aagtccggta
    30421 tcgttttggc aattcattca gtttgctgcc gcttccctgc cggagtgcat tagctgccgc
    30481 ttcctgggcc atttttccgt aactgtcgaa aatatcacaa acaccgctaa tttttatatt
    30541 cagcgcatcc tgctgaagaa agtcttcgta gcgggtatct tctttgtctt ccagggctcc
    30601 tttcttccag tcatcgatgg tggtgggatg cacaaaaccg gcagcctggg caagttgctt
    30661 gccgcggata ccgaagccga tcagtccgat gcggataagt ttgtcgtctt tcgaaacggg
    30721 tttatattca ggagtatcgg ttgaaatatt aagttcattc ctgatatttt cggtgaggaa
    30781 tttttcgtaa ttgcgtttac ggaaccattc aaatccaaag gctcccgcaa cgggaagcgc
    30841 tgcaagcgat ttaagtaagt ctcttctttc catttcaatt cttctttgct aaggtttgct
    30901 tctttgacgg gccgaagata aaacggtcga gtccgaattc acggtttgtc gggaagacat
    30961 atactacggc caaagcaatc agctccagca atattttgtt gacaaccaga tagctgcctt
    31021 cgctcggaac ggcatatttc agccctacaa acggggggtg cgacaggtaa taaagagcca
    31081 ggaggacaat cccgctcaaa caggcgattc gttcgaaaga acccagaatc agggcaatgc
    31141 cgatgagtat cagcccccag atgttcatga agtcaacaat gcgcagcaca tcggggttgc
    31201 cggccagcgt attgataaag aaattcttca ggaagccttc ggaatcgagc agataactta
    31261 cacatgacca gtttgcattt accagtttgg cgagaccttc atacaggaaa tgccagccga
    31321 tggcaatgcg aagcaataca agccagaatt tttgtgatgg tgagtaattc atggatgagt
    31381 atgatgttat aaaattatca gggtagttca agtatcctta ttttcctcca tttgacggca
    31441 acatcgccgc cgctgtgcac ctgcagggcg atgattcccg atcctttccc gatggctgca
    31501 tcggtgaggt cggtcatcaa ctgtccgttg agccatgtct tgatatggtc gccccggagc
    31561 tcgattttca ttgtattcca gttgcccggt ttgaggatgt tttcccgatc gtcaggaatt
    31621 tcataaagcc agcctctgcc gcctgattca tagatgccgc ctgtgttttc tcccggaggg
    31681 gctacctcaa cctgccaccc tttgatgtcg gtgccgttca ggttcgaacg gaaaaatacg
    31741 ccgctgttgc cgtcggcctc cagtttaaat tcaagctgaa ggataaagtt gttgtacttt
    31801 ttttcggttg ccagataacc gtatcccttg tcgggcccgc tggaggcaac gatcattccc
    31861 gctttcacct cccatttttc ggttccgtgg atttgccagc cgtcgagcgt tttgccgtta
    31921 aaaagcattt tccaatgctt tgttccggaa acccggcctg aattgccggc cttctggcag
    31981 tacagatcag gaatacacag gatcaggagt aaagcaacca gtaatggggc gagagttttc
    32041 attcagaagg ttgtagtttc agattatcag caggttatat ataaaggcat gatggccggt
    32101 ttcaaccggg caagataaaa aaaaatgaag gatgaccgga aggacattga aagaattaat
    32161 agggtaaatt tcagcattat ggcgataaag tcagcctgta tactcaatat ttgatccggc
    32221 cgaaggatgg tgcggcgatg atttgatagg gcgttgatta tcaggacgaa tattcaccct
    32281 gttccgaagc gtttacataa tgaagccaga ccaacccttt atcgagggtt tcggctttgc
    32341 acagcttcag atggattttt cctttattta attcatggat cagactgaca gacgattctt
    32401 ttccggcaat aaccggatgg accaggaggc tgatttcatc caccagccct gcttcgagca
    32461 aggcggtgat cagcgcgccc ccgctgtcga cgcggatggt tttgatgttg aattcgctgc
    32521 ccagtctttc gacggctgtt ttgaggtcga cacgctcttt accgcaaaca aggtatttga
    32581 tatgccgttt ttcgagatag tctatgtaat cgcgtggggt gctttcagaa atcagggcga
    32641 taaagtcgcg ccagtaaggg agttgccgta ggtaatgcca gatgcgcacc ctgccccggc
    32701 tgtcgggaat aaccaggacg gctctccggt ctccgggaac cggtaccggc ggtataagat
    32761 ccgcttccgt ttcggggggc gcttcaccag gagctgcaag aatagttgcc gaaccggcca
    32821 gcgtggcatc ttccttccac tgggctgcca gcccgtaata aagttccatg tcgacatcga
    32881 aaccggtgag tttcaaatcg aggcttactg cgttatgaac gatgatgcgg ggtcgcattt
    32941 taaattttct attaccggtg aatgaaagct tgcaggagaa gaacagtcac ggccgaagat
    33001 gccgggcggc gtggtaaaaa ccaacggaac aaaactgcga tcaatttcgg ggtgatgctg
    33061 atcaacgtaa tacaagcgtg atctgaatag gtaaagttag ataaatttca gcctggctga
    33121 tgatgccggt gtgaattatg agacaaacct gtttcacagg catcacaaca tgtaatcttc
    33181 agaagagaaa gagtcttcct gccgaaaatt aatctggcaa agcgggaacc agataagcgg
    33241 cgatcctgcc gttaattggt ttgccaaaga tggctcattt ttcaggttgc gaaattatcc
    33301 ggatacgtat agcgtatgct gaggctgccc ggaaggattt tcttttcccg gagatttctt
    33361 ccctgttctc ccggaacgat catgaatggt ggtatatttc gtctcgctat agagcctcga
    33421 aaggaggatg taatactcct cacatttttc gtggcctact tcatggtcct ggatataagc
    33481 gaacccgccg gcaatatttt cctgttgttt tgcatccagg cttttctcaa ccaggggaaa
    33541 gattgttttc tcttcaacgt ataagtgatc cctgacaagg gaacaaaagt tgtcgatact
    33601 cttcggaagg ctttgcattg agagggattg cccggaaagg aaccggttga acccggtttc
    33661 aatcacggta acgtatgtgc gggcaacgat atgatcttcc agaagtttga ggatcgtatc
    33721 gttgatcttt ggaattttta gtcccagcaa agcggggaaa aggactttct cctctttctg
    33781 attgtggcaa tgatccaggt aaatcttaag aaactcaatg atttgtccga accggaacag
    33841 atcaaccgga gtgtcattcc ggatatcgca ggatatcctt ttcatggcgg tgatgataaa
    33901 cgccatggca tcgtgttcac ccttaaggtc aaatgtagga ttcatggcat atggttttat
    33961 tgcgttatca ggtaataatc aggccggaaa gtgaccggca ggcgaccatc ctgtcgtggc
    34021 gggttttata gtcgcgcgcc ctgatcagga agtcggaaag gacattcatg taattcacaa
    34081 aggcctcgaa aggggagcta tacggactga aataccggtg catggcgccg tcggccgtac
    34141 tcttggtgcc catgtaataa aagtggtcgc atgtctgtaa attattccag tcgctaagaa
    34201 gctcttcatc ctcacactct tccattattt gtgacacgga ataaaggttg ttgaaggctt
    34261 cctgctgtaa ttcgttgcca agccatgccg aaagatcatg ctcctgatcg gcgcaggaaa
    34321 tgacgtcgcg gatgctgaga ttcgcgacag gatgaatggt ttcggctgct tcggatatgg
    34381 ttttcagttg ccatctgttc gatttgaccg ctttttcgag caaagaaccc agaaaagcga
    34441 gtttcccggt gccggagagc cggctttcgc ccagggaacc gtaatccatg aaaaggttca
    34501 cgatctcctc gttttgatca atggcattca gccacgaaat aaatttgtcg gctgtcaggg
    34561 gccattcatt ccagtcgcgg gttgaaaagc gcaatgaaat atcgtcgctg agctggaagt
    34621 tccggagtaa aagttttagg tcgggattgg aattcccgga atacaggtgg ttggaacttt
    34681 tccaccccag ggcctgcctg gcgccttcgg taaagagggt tttatatccc atggaagtca
    34741 ccaggttccc gatttcatcc gaatatacca ggttagtggt aataaatgct gaaggcctga
    34801 agccgaagca actttccatc ctattgctgt gttcagcaac ctgcctccgg tactcagccg
    34861 ggtgggaaag catcgccagg gaatgggcgt atgattcgcc tgtgagttca acattgcctg
    34921 tccggtgtaa ttttacaaaa ctttcaagca catcgggcgc atatctgctg agctggtcca
    34981 tagccgtacc gctcaggctg aaagttatcc tgacggcatc gcggtatctg tcgattaatc
    35041 tgagtaaaat ttcgttcgcg ggcttgtaac aattataggc aacccgcttc atgatctgct
    35101 tattcagcga ttcatcataa taatcgtgtt ttgcgccgat atcaaaaaat ccgtatgccc
    35161 tcagccggta tggctggtga acctgaaata agaggcataa ggttttttta taatcgttca
    35221 gcatcctttt atcctgatta tcttcttttg atttttaaag gtttcagctt taaagccatg
    35281 cgggaagggc cgccggcaaa gccggttgtt atcagccttc accgatagta caaccctgac
    35341 aaagtatctt gtcttaaaac agcaaaagag taatcgttgt ctgttaacgg ggcgctccgg
    35401 gagaacgggc cggagcgctg acgaagcaca cctcatccct ttctgttcac aggaatgatt
    35461 cagccgcgca gaagcaaaac aggatcccct gaccgggatt tccaagttct ataaaattaa
    35521 tactttaccc ccgttattcc aagcaacagg gtaatctaga ttaccggaaa ttgccggtgt
    35581 tttcttccgg attatcacga tcagggacgg gaacggcgtt ttcttctgaa atccctgata
    35641 tttatcagga ttgaaatgat gagcgctgca aaaaatgcgg caagggttga tgtgtcaacc
    35701 agcccggcag gaaggtgcag cccgaacgcg cgggcataca ggtattgcat ataggaggtt
    35761 tcgccgggca tgccgccaag gtggtccagc acctgccagt gccagtcggt aaacgggcaa
    35821 tagccgaacc cgtaaaagat acccagcccg aaccaggacc cgccggtcag cagaagcagg
    35881 atcaggtttg ccttccgggt tttcttccat atccagccga acaggttgaa tatgacgata
    35941 gccaggtgga agaccacaaa aaaatagtcg aggaaatgga accagaacat aaccagggcg
    36001 ttctatgcat ctataaattt acaaaatccg gcgcgaagta accgggtccg ggcgtattta
    36061 ctttaaaagt gcgcgatgaa ttcctccgct gaaaagacta cgtttatact ttttcagtca
    36121 atactttgcc gatagttcac tgatgatgaa tttgtcgccg ccgtcagccg gcagtcagca
    36181 aacttacctc aattttaaaa tattcctgaa gataaattgt acaaggatca ggagctgacg
    36241 tacggagata ttcaggaaat gctgctaaaa ggtgaacctg gcggtaagca ttatttcgaa
    36301 aggcctcagg ctatattcat acaaccagga atagtagctg tttacataca aagaggtata
    36361 ggtgtcggta tcaaaaatat tccggaaacc catttcaaaa tcgattttag gcttgggaac
    36421 ggaatactgg tatacgaaat cagcaaaaaa atgatccttc ttatcgtagg agtaatactc
    36481 cgccgtgaga tttaaagagt gatttttata cgggaagaca aggaggctca gatcatgttt
    36541 aaagatgtaa aaatcgttgc cagtgatatc attgagaata ttgttcatcc aaatggccga
    36601 acctttgtag tcagcggtga tccattttga aaaccggaac atcactccgg gcgtaacagt
    36661 gatattatta ttctgcgtct ccgccttttc cccgttcagc agcgatgatc ctttcacgaa
    36721 gccaagatta aatctgaggc tcagggtggt tctcaacgcg ctgaatgtct ttgccgaata
    36781 gccgttcagg ttgtggatac aggtcctcgc cggcatatac cggtactcat ccaccgaaga
    36841 tccgttgagt gaggtaagcc tgttgcccga atattcataa tccgtcgtaa taaaactgta
    36901 actgatggaa ttaaaaaacg agatgatttg atttctgtat gaaaaatagc tgagaaagcg
    36961 atatttgtca gacaccgaaa ccggtttgtt taccctgttc agttccctga aatttgccag
    37021 aagataggac gggtaataat tactgatatc gtccggctgc agcgaatagg atgcattaaa
    37081 ccttatttgc cagaacgcat ccggcttaag gaccagggtt acttcttctt catgcagtaa
    37141 aagatctgct ttctccctgt tgtcctgagt actatttctc gacagaatcg cataattcag
    37201 ttttccggaa gtggtgagcg tgtattttaa tttcttaatc ttcagttcgg tactgaagta
    37261 gggagaaaaa aggttgcatg cgaaatcatt catgtaatcg ggaaactgac aggattcatc
    37321 cgccgaagaa aacatcccca gatcagaatt aaacgccgtg cttctggcga caaaaccaag
    37381 ttcgggttta atgttgagct taccataagt aaatcccatg gctgcataac cggaggtgta
    37441 aaatttgcct gcagatacat gctgcctgag cgaatcgatg ttattcagca ggaataacga
    37501 atcggagatg ataaccggag taacgtttaa tgtctgattg ccagaaacat aagaaatgtc
    37561 ggtgccaatc tgtaacagct ttttaccgat gggttttata gatttcaacg tatttgaaat
    37621 actgtacgaa gggttttcga gtacctgttg gatccgttca tcattatagt tggtaatgcc
    37681 gttgcaatcg atacccgtga ggttcagcgt cagttgatca ttcagataat tcccgcgggt
    37741 attccggcag aggctaaatt ttgccctgag ttcacggcta tccgatgaat tatcgatatg
    37801 gttgctcgtt acaatcgtat cggccggtaa atagagcgac ctcaactccc gggcctgaac
    37861 ccttcggttg tcatccgata aagacagatt tactttcagt tggagatttt tattcagata
    37921 atgcagggaa ttactgttca acaggagcgt atggttatcc aggtaacgtt cagtcgaaat
    37981 atcgggaaca ggagccgtga tcaggtaaac gtattttctt tgttcagttt caaattcggt
    38041 ttgatcaagc tccgtcaggt tcatatcctg ctgcagaata tcctctcccg cgtcgtttcc
    38101 ctgcaacgaa agtatccact agtttttttt cgtaaaaatg aacggggtta tgttgaaccg
    38161 gtaaagcagg ggggtaatgc ctgcgccaag ccgggcggtt ccggttgtgg caaccttttg
    38221 cttcagtttt agatttaaag cggcttgtga tgaaaattga acatcctgaa gaatccgtac
    38281 cggctgatga ttttcaaaaa cttcgacagc agaaacggag ccttgcggaa ggtttttact
    38341 tactactcca tagttgcctc cggtaatgtc cattccttca acatagaggt tgttgatggg
    38401 tatatcctga taaaggatcc tgccgttggg ttcaaccgta acgcccggca tccgcttcag
    38461 cacatcctca accgaacggt cattctgttg tgcaaatgaa ttgaccagat agctcaatgt
    38521 gtctccgtat ctggcaatcg gctgagcttt aatagtcact ccttctattt cctttgattc
    38581 gggacggagc ataattttaa gctcacggct ttcatttttt atcaggaggg attcgttccg
    38641 gtattgtacc gaagatgttt tgatcagcag gctgtcggat ttggcgttta cctttaaagc
    38701 aaacgagccg tcgttcctgc tgatggcaaa agcctgaata acggcatcgc cgggcttata
    38761 agccagtacg tgtatgtttg ggactccggc cccgttttct gaaagcactt tacctgcaat
    38821 ctgaacctgg ccgaacagag tcattcctgt gatcatcatc cctgcaaaaa ccatcaggaa
    38881 tttaaccggc attttatgca cttgcttcaa aatcttttat ttcctgaaaa gctcaatatg
    38941 gttgttccgt ttagctatgt tttgtgcggc tgtccgggcc gattcgtcat cggcgccttt
    39001 gtccttaaca atattgatgg cattgtcagc catggaattt ttagctttaa aaaactgttc
    39061 ctttgtgctt tctgtataga ttttctgctc tatgtctatc aggatttcac cggccggaat
    39121 actgatgctt tccatctgaa gaacataatg gttgcggctg tcgtacattt ttacaatgag
    39181 tccgggcagt ccgttaaact tataaggccc gtcactgatt gggatttccg gacaaaacca
    39241 ggccgtccag ttccggcccc caaaaacaca ggttgctttc tgaacccggt aattgttgat
    39301 ggtagctgtg tcaggttcca ttacccagtc aaaagcgttt aacggttcga tgtatttgta
    39361 ttgatcaaac agtaacggct cgatgcaggt taatttcccc ggcggataat ttttataaat
    39421 ccggtaccgg gtagcagggg aaggtttgtt cggattgctt gcaaaagttt gtaaatcctc
    39481 atgggtttga aatttcctca tgatcgtatc actaatatac aggctcctgc tttcatacaa
    39541 actgatactg tcgccgacaa ataaaagcat cctcccgttt ttaacattac cggggttgtt
    39601 tgaatccggt accgattgct gggaatattc gatgcagtac tttactttat ccagaactgt
    39661 aaattgcagg tcgaataatt gggcgacagc catgtttggg cagtgaagtg cgatggtaag
    39721 aataatcgcg aaggcaatcc ggaatttatt gcaggacata tacatagaat atttaattt
//
LOCUS       DAOPYV010000026        39432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_899_length_39432_cov_13.412474, whole genome shotgun sequence.
ACCESSION   DAOPYV010000026 DAOPYV010000000
VERSION     DAOPYV010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 39432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39432
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_899_length_39432_cov_13.412474"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..1250)
                     /locus_tag="PKI35_05050"
     CDS             complement(45..1250)
                     /locus_tag="PKI35_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNX43786.1"
                     /translation="MTKSNDNPMTLFTASGCLTAGALERYVSGGLPAGDLSRVKAHLD
                     ECEFCSAALEGLTAWREQNIVTEYHHDAPGNHRASSEIHHNKNFSRRVELINERLHER
                     VKFHQETAPEKQKRRIGPVPSWLAMAASIVLFAGIYLILQNHKPSREFVATEQSGEKS
                     LMPRPALAPDSLTTLSQPIVVAENKPAKKRAESQESMTPESMEIIINNDEIAEEPFVA
                     GLVSKEQVQPPADKSVFPVAGVAGNAETISEYRMQEARNARSGTAWEQAAQEPETAKR
                     VREAPLSAVQATENEASEEESVVFTVVEESPEFPGGADKLNRYLSENLHYPAQARENS
                     ISGTVFVRFVVDEKGKITHAEILRGIGYGCDEEALRVVKGMPRWKPGQQRGKPVKTIF
                     NLPVKFNLQ"
     gene            complement(1247..1858)
                     /locus_tag="PKI35_05055"
     CDS             complement(1247..1858)
                     /locus_tag="PKI35_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX43787.1"
                     /translation="MAFNLFGNYRDKKKELQSLSDEQLAVRYARTGDTEYLGQLYERY
                     THLIFAVCLKYLGNEDEAEDTVMLIFEKLVKELKNESVSAFKGWLYTVTRNECLMQLR
                     HRKTVEKSKEVLLKESEAGIMENDSVLHLITGNNSEDDHKLEEAISRLNEDQRRCIEL
                     FYLEERSYREVAETTGFTLNEVKSHLQNGRRNLKIILENVRKP"
     gene            2043..2738
                     /locus_tag="PKI35_05060"
     CDS             2043..2738
                     /locus_tag="PKI35_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNX43788.1"
                     /translation="METKKSNRADLENKKLIFLQIGLIVSLGIALAAFEWESPDRVKI
                     DYEGVLGIPLDQDLTSIQTTHPVLPPPVMIKPATIFNLVDNKTEVEDLTPPDFGDNPE
                     DIAPAYTPVAKPVLQDEKPDSGEEVFISAEKMPEFPGGEREMLRFIGDHISYPLAARE
                     TGIQGTVYVNFIIEKDGSVSQVKVLRGIGYGCDEEAMRVISLMPRWSPGMQRTIPVRV
                     SFNMPILFKLKDS"
     gene            2769..5129
                     /locus_tag="PKI35_05065"
     CDS             2769..5129
                     /locus_tag="PKI35_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNX43789.1"
                     /translation="MKTRLTIGIASGFIPVLILSAVTMIFSQNTGRMYPPVKPTIPAE
                     EPDSQDKTLSPYFFVKSDDPALDQLPLKATSASVNIAGVIADVAVTQEYANEGKRPIE
                     AVYTFPASTRAAVYSMKMTIGERTIVARIAEREKARKDYEEAQRQGKSASLLEQQRPN
                     VFRMNVANIMPGDHIKVEMKYTELLIPEDGVYEFIYPTVVGPRYSNKPEALASADEKW
                     VANPYTHEGEKPFYSFNIQTSIAAGLPVTEIVCPSHRTNITFDGPSTASVLLDPSEKS
                     GGNRDYILRYRLSGNSIETGLLLYPGEDENFFLAMIQPPKQVKQADIPPREYVFIIDV
                     SGSMNGYPIDISKKLMHDLTGSLRTSDRFNVILFAGCSSLFSEQSVPATPENLKRAIA
                     FIDREQGSGGTELLPALRRALALKGTESYSRTFVIATDGYVDVEREAFDLIRKNLDKA
                     NFFSFGIGTSVNRYLIEGMAHAGSGMPFVITKPDDASVQAERFRQYIGNPVLSHIKAT
                     FKGFEVYDVEPSSVPDVFAERPVIIFGKYRGIPGGTIEIDGTGGQGKFERKVNVAGAK
                     PSASNSALRYLWARERIRNLDDLAAVGSAEEQKEAVTMLGLKYNLLTRFTSFVAIDSE
                     VRNKEGDATTINQPLPLPEGVSDYAVSGITGSAAPMMMKSSPDRMACEEVTINEKEEK
                     ENEVFVVAETMPEFPGGEKAMKKFIADNLIYPPLAQERGIKGKVYLSFLVEPDGTITN
                     IRVLRGLGGGCDEEAVRVVEMMPPWSPGMQRARPVAVQYNLVVEFK"
     gene            5427..8660
                     /locus_tag="PKI35_05070"
     CDS             5427..8660
                     /locus_tag="PKI35_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX43790.1"
                     /translation="MNKIEIHPILEVPVNEKVTFQFEGKPVTGEKGFTIAAALHQAGY
                     PVHSHSLTNRERSLECGIGKCGACEMLVDGQIRRICITKVDGVKEVMEIPRDYTPEIR
                     ERDAQKPINIYKTTVAIIGAGPAGLACREELLKKGIDNIVIDNNDKIGGQFLMQTHQF
                     FFFEKEKKFGGMRGFDIAKTLAGDSHEGIFLNSTVWDVLEGKRIAVKNIRTEEIYYVD
                     AEYMVVATGAVPFMPTFENDDLPGVYTAAVVQKMMNNEFTLLGKNVLTVGAGNIGYLT
                     SYQLMQAGAHVKAIIEAMPGEGGFPVQANRVRRLGIPVLTSRILLKAIPNDDNTGIVG
                     AVIADCKNFEPVAGTEKVIEGIDAINICTGLVSDDQLIIKGNEIFGRNCYGAGDAIRI
                     GEGTSAVVRGKQVAMEILQEMGENINYDEYLTVSKEYIDSQQHPIRVIQEPFKPSAER
                     FNEKPFVQIDCLYGFACNPCEFACPHGAITKTSTSTVPHIDFGKCIGCMECVHQCPGL
                     AIFGYAPKKDWIFLPIEYFVEENEEVFLVDNQGKKLGTGVIEKVLKKPNKTNVARVKA
                     HDIHGEALMQVRGFILRKNYPEPVEMAPTSFESEHEMYVCHCDDVTLGEILNTIGDRK
                     FISVDEIKHTTRLGMGACRGKRCIRRLKQVLRSKGISIVGDATPRGPLSNQVSMGELF
                     PKEVRENIITNGKNARRRVDVQAVIAGGGMAGSALFRYMAEAGLKPALINYGRGSSWR
                     NIAGGRPAFSIPEIADIARHNYDIFRELQTIRDVNFRSTRYVTFAHDEATCKALEDSR
                     SWSDAYMVDPKDFAKEIAPNINPSLGDIYPAALITRDCWQATPGRVIDLVRRIGIEKG
                     GKVWEDCQLIDVRKENGEYVVLARDHEKHYIEFHTPHFVNSLGPGADKFAKQLGINTG
                     FYPVKHQAFITRRLPNMGIDGQPLDMLIDRRRYKGFSAVYGQQLGETGQIIGCASPLV
                     EPMEVDKNLKVNSKDFLEIVSEIFVQWLPQLSSVGFQAVWAGYYVEPRMIVDVDAGLL
                     VGLSGHGFMLGQYLAKMYTDKMLGKPVPDYLERLSVNGDGLTEKAFK"
     gene            complement(8727..11219)
                     /locus_tag="PKI35_05075"
     CDS             complement(8727..11219)
                     /locus_tag="PKI35_05075"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNX43791.1"
                     /translation="MSNPIQNFPEPDFQEQSIDYKAIFFRFYNYWYLFLITIFVSLLI
                     AFLFNKYTRPEYKVKATVLVKDEGGGISDAQALLGFGSITNTQKLQNQIGILNSRSLK
                     TRTLKALHFEVTYSREDNFITNEIYTESPFLVEFDTGHVQPVSNMKFYVTLLSKDEYE
                     LSCEGQDVELYKYNNYTDAGKKEGLVKFSKKARFGEQITTPEFSFFLRLSNKFKEQKH
                     LGQQYFFTFNSLNSLISAFSGYEVEPINKDASIVEISLKGNTSQKAVDFLNQLTREYI
                     QQGLDKKNQIAINTINFIDTQLLEIGDSLSHNETRLQNFKTVNEIMDFDAQTQEAFTH
                     VKDLETQKAQLLVEAKYYKYLQNYLAAKQQNLDSLVVPTSMGINDPGLTKLIGELTAL
                     YAERAERLINTKEGNPLIMALDQKINNTKKSLTENIRNIVNTSNIAVSDINDRISKYS
                     GQFRQLPATQRELIGITRKYKLNDAIYTFLLQKRSESAITRASNTPDNESVDMANDEV
                     KEKVFPKTSLNYTIALVLGLVLPILYVLGKDFLNDKIVDKHDIENATKLPLIGHVLHN
                     RRESTVVVATSPKSSIAESFRALRTNLQYITQGKDKITILVSSTSVSEGKTFISMNLA
                     AVYAQYERKTLLMGFDLRKPKIYQDFGLTNTAGITSYLIGKCSLEEIIQKSPFDNLDV
                     IMSGPVPPNPAELIASPKTAEMMEKLKEMYDFIILDTPPFGLVTDAFLLMKFVDAKIM
                     VVRQNLTNKKVFGSIAADIENRNIPNMVVLMNDVKPEKSGYYGYGYGYGYGYGYGYGY
                     GYGYGYGYGYGYYSDDADTEKDTFFKRLFRKS"
     gene            complement(11225..12010)
                     /locus_tag="PKI35_05080"
     CDS             complement(11225..12010)
                     /locus_tag="PKI35_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNX43792.1"
                     /translation="MKNISRFVRPALLLAVMSLALFSCVPQKKIRYLQTAEGADTVRV
                     FRNVSAAYLLQPGDYLYIRIFTLDEKSNTLFNSISGSSYMGGSYTDQYTYLTSYMVND
                     SGYINFPVAGFIRAAGLTTGQMEQELHKELTEYIKEATVVVKLVSFKISMLGEVGHPG
                     QFTINRDRINIFEALAMAGDLNTFANRQKVQIIRQDKQETKVVTFDLTRKDILNSEYF
                     YLRPNDIVYVEPLKGKQFAFETFPYSLIYSTISLAFLVLTFFK"
     gene            complement(12372..12791)
                     /locus_tag="PKI35_05085"
     CDS             complement(12372..12791)
                     /locus_tag="PKI35_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43793.1"
                     /translation="MEYLSADHPEYKAYSLVLPGLPQYEYKNSGWKGWNDWLGLNTEF
                     KDYETTRRFVISLNLKSSRDWKNYSQAKRPQHLYAYPDIAYQNRGWRGWDHWLGLDSV
                     TDRKSHPVPDIPEGARPCRCAGLNEHCTDCDGKGYYF"
     gene            complement(12910..13503)
                     /locus_tag="PKI35_05090"
     CDS             complement(12910..13503)
                     /locus_tag="PKI35_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43794.1"
                     /translation="MRKTTVILVVMFLQSCMVMINENDYRMLQETDKSMFKPYSAVSE
                     SGSGNTGDLFLYEINTPQMLQRMKYNHYTWIHLWRPYCTSDYCTHIDYFFNLAGKYTL
                     KGLDLLFISECYGVRNIKQKLADTASHHTFYVLEDKYYGHKLNPARQKFMKDLGIEIT
                     KQNKWGYDDYIFKDTTLIFKGDHIGETTIDSLISLYH"
     gene            complement(14390..16111)
                     /gene="lysS"
                     /locus_tag="PKI35_05095"
     CDS             complement(14390..16111)
                     /gene="lysS"
                     /locus_tag="PKI35_05095"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNX43795.1"
                     /translation="MAFELSEQEQIRRNSLQELYKLGINPYPPELFPVNVTSAEILGN
                     YPGDPALYQDIYIAGRIMTRRIMGAASFVDLQDSAGRIQLYVKRDEICPGEDKTLYNT
                     VFKRLTDIGDIIGVRGYVFVTQMGEISIHVKEFKILSKSLKPLPIVKEADGQTFDAVT
                     DAEFKYRQRYVDLVVNPSVKEVFMKRTRMINAIREFLNSQGALEVDTPVLQSIPGGAA
                     ARPFITHHNALDIPLYLRIANELYLKRLIVGGFDWVYEFARDFRNEGMDRTHNPEFTI
                     LEFYVAYKDYFWMMEQTEKMLEKVAMELHGTTDVAIGDKVISFKAPFRRIGILDAIKE
                     HTGMDVYGMDENALREVCGKLEVEINETMGKGKMIDAIFGEKCEGYYDQPTFIIDYPV
                     EMSPLTKKHRSKPGLVERFELMVNGKEIANAYSELNDPIDQRERFEEQVRLMERGDDE
                     AMFIDYDFLRAMEYGMPPMSGIGIGIDRLAMLMTNQASIQDVLLFPQMRPEKIAAPVD
                     NDNEFLALGIPADWVPVVRKAGLKTAADVKAANPNKLLNDLSGLRKKLKLEIPALKIE
                     EVKAWCE"
     gene            16264..17775
                     /locus_tag="PKI35_05100"
     CDS             16264..17775
                     /locus_tag="PKI35_05100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNX43796.1"
                     /translation="MKFSHLTDSFKTTYFRWIVMIMLFIPLLFISNRRSHDWGDDFAQ
                     YIHQAKNIVQGIPQSETGFVYSQENFIGPKAYPVGFPLLLSPVYAVFGNNMKAFTTYI
                     SVFYFILALLLVLFFRKYFSLIPSVVLAMILLYNPQMMLFKQEVMSDLPFTVLLVASM
                     LLYMNFRKNNTVKIILVLIAGLMITVRPIGFVFLIAVLIDQVVMLLKEDCSRRRKFYE
                     LVVATVFVLLTSGIYLLLNVAIFRIPSGGGLNNYLAYFGSGEIVSTILTNLRHYSEVF
                     QNFYTPSLGDYDGFSLLSGVLFLAMAMVGLLKRFTTRIEFLDIFFLIYLVILLVFPNN
                     ASAFRLMVPVSFLLLLYVATGFRSLSVLPSVSGKLKAAILGILILAFYMPGIRDIIST
                     KYIIMAGPQKAESEQAFDYIRENVPENAVIMFVKPRALSLYAGRAGFADPAETDMTKF
                     HKQVVDAGVNYFLLNNDISSEASKRYLRVMNERVDSVWSNETFGLYRLKSFNP"
     gene            complement(17765..18493)
                     /gene="recO"
                     /locus_tag="PKI35_05105"
     CDS             complement(17765..18493)
                     /gene="recO"
                     /locus_tag="PKI35_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNX43797.1"
                     /translation="MLIVTKGIVLHTVDYSETSVIVKVFTEQFGMQSYLVKGVRKARS
                     KIKRNLFGPLSLVEIVAYRKENTGLSVARDVTCHVQLNHIASDIRKMAVAVFINELVY
                     RSIQGEMPDQQLYNFLYRTVLDLDSADISVAGFHLVFAMQLAHHLGFGPHDNYTPANN
                     FFDLQEAYFRDHPPDHPYYLAEPLSRFWHELIGFPDGRALPQDFTYSVRKALLDRLMD
                     YFRLHVPAFGEMKSHHVLNEVFRD"
     gene            complement(18695..21031)
                     /locus_tag="PKI35_05110"
     CDS             complement(18695..21031)
                     /locus_tag="PKI35_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX43798.1"
                     /translation="MLMFSGKIHAFILVISLFIAGALSAQSSGIGEWQDYLPYNNCKA
                     VVQGGSRIYCATPYSLFYVDKTDNSITRLNKVTGLSDVDISAIAFSKSLNTLIIAYAN
                     ANIDLVKNDVVINIPDIKRKQILGNKTINSITVIDNYAYLACGFGVVVLDIDREEIHD
                     TWYIGPEGDQINVNAITFAFDKYFYAATDAGIYHADASSNLAYYINWTKDNSITDPSG
                     KYNYIAWHGGKLYVNSTPDFSSFSKDTMLVYDGSHWEYFQPANHSTRAGFRVMNDRLL
                     VCNYLAVDAFKPDGTLDYRMYSYNPGAIRPNDAILDDENKVWIADNDNGLYKMTDQWN
                     GVQILINGPVSALVTAMDVSGNDLWAVPGGHDNGFGNLYNLARFYYNIDGNWSSFSYL
                     NADFLKDMRDIVCVAVDKKNPRHAFLGTWGYGLIEFNDGQLVTIYDKSNSSLQGSVYD
                     TSLVFAGGIALDKDNNLWVTNNTAPEILSVRKADGTWRSFNPAPGSSTYYTGGIVIDK
                     AGQKWLQMRDHTLVVFNDNGTIDNPADDQGIFLTGTAGNGALPGTGAILSMAVDNDGQ
                     LWLGSDQGVAVIYSPENVFNGGNYDAQVVQVEVDGYLYNLLETESITAITVNGDNEKW
                     IGTGKAGVFLMSADGSKEIHHFTAEDSPLLSNTITNIRIAADGEVFFGTSQGIVSYRD
                     YKIAPDSSLNSVIVYPNPVKESYTGNIAIKGLVKDSNIKITDISGSLIWETQAEGGQV
                     IWDGKNFSGRRASTGVYLVFISNPDGSQTQVAKILFVH"
     gene            complement(21247..22002)
                     /locus_tag="PKI35_05115"
     CDS             complement(21247..22002)
                     /locus_tag="PKI35_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX43799.1"
                     /translation="MQKLSIIIPAYNEGKTIHLILDRIRRVELIEGIKKEVIVVNDFS
                     KDNTEEAVLNYKADHTDLDLQYFKHEVNKGKGAALHTGIRVATGDFIIIQDADLEYDP
                     NEYNILLKPVTDGAADVVYGSRFMGGKPHRILFFWHTIGNRFLTLISNMFSNLNLSDM
                     ETCYKLFKSDIIKNISLHENRFGFEPEVTQKVARIKGIRIYEVGISYYGRTYEEGKKI
                     SWRDGFRALYCVMKYGMFKRNNKQTKSAGTASR"
     gene            22361..22930
                     /locus_tag="PKI35_05120"
     CDS             22361..22930
                     /locus_tag="PKI35_05120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX43800.1"
                     /translation="MIHYSDFAIIEGLRLNSDFIIRYVYKEFFPTIRYLIKNNAGLDE
                     DAEDIFQESLYIVLKKIQEEDFELNSSFLTYLYSISKNLWLQRLKRKRLHEKNQSDLV
                     HFLSSPDTGVEVHIEYEEKYRVFIEQFENLSEDCKKLLHLFLGKMSLKEIADEMGYKS
                     ELYAKTKKYLCKEALRKLVENDPAIRSMK"
     gene            23067..24761
                     /gene="ispG"
                     /locus_tag="PKI35_05125"
     CDS             23067..24761
                     /gene="ispG"
                     /locus_tag="PKI35_05125"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HNX43801.1"
                     /translation="MSNFEDLFFSPVRLRFQTREVFVGDVPLGSNNPVRIQSMTNTDT
                     NDIEATVRQTMQLADAGCEYVRITAQGEREAANLAVIRKTLHSRGCRVPLIADIHFSH
                     RAAEVAASIVEKIRINPGNYADRRHDVHIHYTDREYGLELERIAERIHPLLKICKENG
                     TAMRIGSNHGSLSDRIMSRYGDTAEGMVESALEFARICEDAGFRNLVISMKSSNVRVM
                     VHATRLLVDRMMAEGMAFPLHLGVTEAGDGEDGIIKSAAGIGSLLNDGIGDTIRVSLT
                     GDPLQEIPAAHAIAQRYNVMNSPACVALSHHKPENRNSVGADQCSFNPFSFRKRETIA
                     TGNIGGNRPVQVLAGKNGILSLVDEYDSATGAVTEQAFSGLIVQEEEVRAFRSKILSL
                     PVSDQRPVVLTKEYQTNDLKSFTIDSAIDFGSLLIEGIGEAIWPVAPNIPGEETTRMA
                     FGILQATRTRITRTEFISCPSCGRTLFDIASTLQQVKQKTGHLKGLKIAVMGCIVNGP
                     GEMADADYGYVGAGRGAITLYKGKEVVKRNIPETEALNMLINLIKESGDWKEPENS"
     gene            complement(24820..25749)
                     /locus_tag="PKI35_05130"
     CDS             complement(24820..25749)
                     /locus_tag="PKI35_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNX43802.1"
                     /translation="MDVEWFVIVNPNAGKRKGQHDWLSIAHLLEEAGIGYTNIFTEHR
                     GHAMKLTRRYIENGFRKIIVVGGDGTLNEVVNGIFTQDHANPRDITLAMIPVGTGNDW
                     CRMFGIPLNYKDAINLIVRGKVFLQDIGVVEYISSEGLQKKRHFANMAGMGFDALVAK
                     KTNIQKEAGKGNPLSYFVNIFSSLFSFKVTRTQITLDDREIRSGVFSMSVGICQFNGG
                     GMKQSPFAVPDDGLFDLIIISPIGKFKAIRNVLNLLDGSFTKLPEVSSFKSAFISIES
                     DPPLYLEVDGESLGHSPFEFNIKKQSLHIITGK"
     gene            complement(26008..27342)
                     /locus_tag="PKI35_05135"
     CDS             complement(26008..27342)
                     /locus_tag="PKI35_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNX43803.1"
                     /translation="MNSDTPSLKRIWEIAWPIMISLLAQNIVGVTDTAFLGRVSEVAL
                     GGSAIGGLFYIILFMIGNGFVTGVQILIARRFGEKNFGEIGRIFDNSFYFLAVTSIVV
                     TVFIYFLGSVILKPIVSSTAIFEAASTYLQYRVFGLFFAMIGLLYRSFYTGITYTRYL
                     SISSAIMAVVNIVLDYLLIFGKAGFPEMGLTGAAIASAISEAVALVFFIIATNRSERV
                     KPYGLFRFLKPDPAIIRNTLGISIFVMIQFVLSLGIWFVFFLIIEKMGERPLAVSNIA
                     RSVYMFLMIPGWALCSVTNTLVSNAIGEGKPGQVLPVTYKIMRFSVVMLLAVVSVAAF
                     LPVPILGIFTNDAGLIKAAIPTFYIVLVALFFFSLVSVLFNAVLGTANTSVSLFIEII
                     TLTFYVGYTWLMAIRLHQPVEVAWTAEWVYALFVGVLSFVYLKYGKWRQKVV"
     gene            complement(27419..28213)
                     /locus_tag="PKI35_05140"
     CDS             complement(27419..28213)
                     /locus_tag="PKI35_05140"
                     /inference="COORDINATES: protein motif:HMM:NF007757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNX43804.1"
                     /translation="MDNLSITLIQPDLAWEDKPGNLAKFEGIISKLKTKPDLVILPEA
                     FSTGFVVEPHSVAEEAEGQTFRWMQKISADHGCAITGSCIVKSGDDYYNRLIWMRPDG
                     SSEYYDKRHLFRFGDEHLQFSPGDQRMVVELKGWKIRPLICYDLRFPVWSKNRYFNNS
                     YEYDLLIYVANWPGKRSYAFRQLLIARAIENQSYVIGVNRVGRDGKGTLHQGDSAVLD
                     YRGKPIIEIPPDVEDVETVQLSYEKLHEYREGFKVALDWDDFSIGK"
     gene            28561..29817
                     /locus_tag="PKI35_05145"
     CDS             28561..29817
                     /locus_tag="PKI35_05145"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNX43805.1"
                     /translation="MKTIDDYTFTGKRAIVRVDFNVPLDENFNITDTTRIDAAIPSIS
                     KILASGGAVILMSHLGRPKGPSKKYSLTHILPYLSKKLGRDIKFADDCIGQSAADQAA
                     ALKPGEVLLLENLRFYEEEEGKPNLPDTATDEEKKAAKAKVKESQKEFTKKLASLADC
                     YVNDAFGTAHRAHASTALIAKYFPNDKMFGYLMRDEVASIRKVMHEGQRPFTAVLGGA
                     KVSSKIEIINNLLGKVDNLIIGGGMMFTFIKAQGGKVGNSLVETDLLELAKEILAGAK
                     TKGVNLILPVDAIAASAFANDADRKLVKAGEIPDGWMGLDIGPETVKLFSEVLNNSKT
                     ILWNGPMGVFEMDNFQEGTKELAMAVANATGKGAYSLIGGGDSVAAINKFHLADKVSY
                     VSTGGGAMLESIEGKELPGVKAILED"
     gene            29925..32264
                     /locus_tag="PKI35_05150"
     CDS             29925..32264
                     /locus_tag="PKI35_05150"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNX43806.1"
                     /translation="MKNLLLLSVLISFMSLSGFAQSESATIQTSTGVQDYCNYVNPFI
                     GTLRMGHTFPGATVPFGMVQLSPETNNPPMYINGKYNADVYKYCSGYQYDDSTIFGFS
                     HTHFSGTGHSDLGDFLVMPTTGKLNLEPGDATKPHSGYHSQYSHEKESAQPGYYSVHL
                     DDYNIGAELTATERVGFHQYTFPETDSAHIILDLISNIYPYDEKNVWTFVRVENDSTV
                     TGFRTTSGWARTRTLYFVMKFSKPFVSYGHKKYDKVPYTGFYRRFNENENFPEMAGRK
                     IRAYFNFKTSGNEKIRVKFALSAVSVEGAMKNLEAEIPHWDFEKTKAESRAKWNRELS
                     KVQVETLTPEDRVTFYTALYHTMLSPVIFEDADGRYRGLDQEIHTSDGFTNYTIFSLW
                     DTYRALHPLFNIIQPARNADMINSMLAHYDQSVHHMLPIWSHYANENWCMIGYHAVSV
                     LADAMAKDIAGFDRKKALEAAVSTSTRGYFDGLDYYMNKGYVPEDKSSSSVSKTLEFA
                     YDDWCIAQIAKENGNERYCQEYRKRSHNFENHYSPASVFLRPRLADGSWKQPFDALDT
                     HDQGYIEGNAWTYSLFVPHDIDRLIEMNGGNAKFIAHLDSLFTMKLDDKYIEKTEDIT
                     RDGIIGNYVQGNEPGHHIPYLYNWAGAPWKTQERVRMIMSTMYGNKADGLCGNDDAGQ
                     MSAWYIFSALGFYPVIPGSDQYAVGSPLIKNAVINLEDGKSLVIKTMNQKAGNVYVQK
                     ILLNGKELNRRTLTHAEITGGGELVYYMSAKPDRKIFIK"
     gene            complement(32273..32821)
                     /locus_tag="PKI35_05155"
     CDS             complement(32273..32821)
                     /locus_tag="PKI35_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNX43807.1"
                     /translation="MNILDIIILAPLAFFAYKGFARGFFISVAMLAGLLFGFYAAINF
                     SEFMAVFLTNKLNLTSSNIKFIAYITTFVLVIVLVYLLGQFLSKIARTAGLALVNRLG
                     GAAIGAAKGLIIVSAFLLVLVKIDPKAHLVSQQTRNESISFKPVLSISPAIYPMLKKY
                     SGKVLDMMNHDDAGSEKTSKVP"
     gene            complement(32901..33653)
                     /locus_tag="PKI35_05160"
     CDS             complement(32901..33653)
                     /locus_tag="PKI35_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX43808.1"
                     /translation="MIEGININKKFADRVVLENVNVSFDKGKTNLIIGQSGMGKTVLV
                     KCLVGLIEPDTGVVRFDGRPFSKMSYSEMKQIRQEIGMLFQGGALFDSMTAVENVMFP
                     LTMFTAMTYEEKLERANWCLSRVNLVNVNKMLPSELSGGMKKRVALARAIALNPKYLF
                     CDEPNSGLDPVTACLIDELIGEITHESDITTVVVTHDISSVLKTGEKIYFMYDGRITW
                     QGDRNNILQTDSPELQNFVFASELTRRLKPRQ"
     gene            complement(33650..34390)
                     /locus_tag="PKI35_05165"
     CDS             complement(33650..34390)
                     /locus_tag="PKI35_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX43809.1"
                     /translation="MNFFYHLGRYMQMLQQAFQKPEKRSIYYRQVLIEMEHLGLASVG
                     IVAIISVFMGAVLTIQLAFNIDTPFIPLYTIGFSVRQSVVLEFSPTIISLILCGKVGS
                     RIASELGTMRVTEQIDALEIMGINPATYLILPKIIASMIINPILIALSMIVAVFGGWL
                     SGILTGLVPSYQYVYGIQSFFHMYDIWYAIIKTIVFAFLIASISAYHGYYTRGGALEV
                     GEASTNGVVRSSIAVICMNLVLTQLLLS"
     gene            34693..35319
                     /locus_tag="PKI35_05170"
     CDS             34693..35319
                     /locus_tag="PKI35_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43810.1"
                     /translation="MYKKVDPLQAIRQYFPPETFKSVEACFRNYRFDLRISRPRKNKL
                     GSFHPVAGINNPLITVNADLGKYMFFLVFMHEIAHLIIHQKYGRSVLSHGEEWKAAFR
                     RLVIPLMDNRQLPPDLTAPLQQYFITTPATFIRQTELLRVINRLDGKGHVDTLTDIPV
                     DAIFALSNGKRFVKLEMKRTRCRCLCLDNKRYYDVACNAQIFPVSDTH"
     gene            35389..36486
                     /locus_tag="PKI35_05175"
     CDS             35389..36486
                     /locus_tag="PKI35_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNX43811.1"
                     /translation="MIMNQNNYCIIMAGGVGARFWPLSRTNRPKQFIDILGTGETLIQ
                     QTFRRLSASCPPENIYIVTHENYRELVLQQLPNADPQKVLYEPVRRNTAPCIAYACHK
                     IAAENSKANIVVAPSDHIILREDIFTDVINTALEAASANDWLITLGITPSRPDTGYGY
                     IQFNDSKTYGPGGRLNKVITFTEKPQYDLAVSFLQSGDFLWNSGIFIWSVQSILKAFE
                     QHLPEVDSLFREGKNVYGTPAEKEFVNKAYTVCQSISIDYGVMEKADNVYVVAADFGW
                     SDLGTWGSLFDARIKNEDGNSIEGSNILTWDTKNSIILMPENKLAVIDGLNDYIVAED
                     NNLLLICRRGDEQRIREFVDTIRHDKGEKYV"
     gene            36546..38696
                     /locus_tag="PKI35_05180"
     CDS             36546..38696
                     /locus_tag="PKI35_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNX43812.1"
                     /translation="MKKYLLLLAILVSLQMKSVADEGMWLPLLIERLNYVDMQKEGLH
                     LTAEEIYSVNHSSLKDAIIMFGGGCTGEIVSPEGLIFTNHHCGYGSIQSHSTVDHDYL
                     TDGFWAFRKEEELPNPGLTARFLISIQDVTTTVLGSVNDNMPESERNEKIKKAIREIE
                     ENFSKGTKYNARVSSFFNGNEYYLFVYEVYRDVRLVGAPPSSIGKFGADTDNWMWPRH
                     TGDFSIFRVYTAKDGSPADYSPDNVPMKPKHYLPVSLKGVNKGDYSMIMGYPGSTDRF
                     ATSYTIDWAVNKHNPAVVKIRTEKLGIMKKDMDADPAVRIKYASKYAGTANYWKFYIG
                     QNKGLKRLDVEQRKRETEERFSEWINANRDRNLLYRDALYSISKAYETLNRYELAMTY
                     NNEAINRGCEIISFSRQFNSLAIELQAKNPDQEAIEKIIGRCRTSSDQYFKNYNAGTD
                     KQLLAAMLNLYFTNVPRDQQPAYLKTLYSKKTNSFSDYAARVFSKSIFADQQKLEAFL
                     QNPNLKKLQKDPIWPLQIAFAENAGKIDSLTRPADELLSRGKRLFVRGLRQMEADKSF
                     YPDANSTMRLTYGKVDDYSPQDAVDYDFVTTLDGIMQKEDPSSWEFVVPAKLKQLYQD
                     KDYGRYGKNGKMVVGFITNNDITGGNSGSPVLNADGELIGLAFDGNWEAMSGNYAFEP
                     DLQRTICVDIRYVLFIIDKFAGAKNLIDELTLKE"
     gene            38854..39354
                     /locus_tag="PKI35_05185"
     CDS             38854..39354
                     /locus_tag="PKI35_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNX43813.1"
                     /translation="MAIETERKYLVRTCLLPPLGDGKQIVQAYLHVSKQVTIRARIEG
                     ESAYITVKGFQDKLSRPEFEYPVPIDDAIFMINSMSPYPIIRKIRREIFCHGKCWQLD
                     EFQEMNAGLWIAEIELTDPDEPVELPPWVSDEVTGDPRYLNSGLSINPYCNWNNGEKM
                     DNLLNT"
BASE COUNT        10448 a         9566 c         9419 g         9999 t
ORIGIN      
        1 ccttagcggc aggaaatgaa gatcagggaa tcagcgttca acccctattg caggttgaat
       61 ttgaccggta aattgaaaat cgtctttacc ggttttcccc gttgttgccc tggtttccag
      121 cggggcatcc ccttcaccac gcgaagcgct tcttcatcgc agccgtaccc gatgccgcga
      181 aggatctcag catgggtgat ttttcctttt tcatcgacga caaagcgcac aaatacagtt
      241 cccgaaatgg agttttccct tgcctgtgca ggataatgca ggttttcgga gagatagcgg
      301 ttcagtttat ccgcaccgcc gggaaattca ggggattctt ccacaaccgt gaaaacaaca
      361 gattcctctt cactggcctc attttcggta gcctgcacag ctgacagagg cgcctctctt
      421 acccgttttg ccgtttcggg ctcctgtgcc gcttgttccc acgctgtgcc gctcctggca
      481 ttcctggctt cttgcattct atattccgaa atggtttcgg cattgccggc aactcccgca
      541 acgggaaaca cacttttatc tgccggaggc tgaacctgtt ccttcgaaac caacccggct
      601 acaaaaggtt cctctgcaat ttcatcatta ttgataataa tttccatgct ttcaggagtc
      661 atgctttcct gggattcagc ccgttttttt gcaggcttat tttcggcaac gacgatgggt
      721 tggctgaggg tggttaacga atcgggcgca agggcgggcc gtggcattaa tgatttttcc
      781 ccggattgtt ccgtggcaac gaattcccgc gagggcttgt gattttgcag gatcaggtag
      841 atgcctgcga aaaggacgat gctggctgcc atagccagcc atgacggaac gggtcctatg
      901 cgccgttttt gtttctcagg agctgtctcc tgatgaaatt tcaccctttc gtgaagccgt
      961 tcattgatga gctccacccg gcgactgaag ttcttattgt gatgtatttc tgacgatgcg
     1021 cgatgattcc cgggagcatc gtggtggtat tcggtgacaa tattctgctc ccgccaggcc
     1081 gtaagccctt ccagcgccgc agaacagaat tcgcattcat cgaggtgcgc tttcaccctg
     1141 ctgagatcac cggccggtaa tcctccggaa acataccgtt caagcgctcc ggcagtcagg
     1201 cagcccgaag cggtgaaaag ggtcattgga ttatcgttag actttgtcat ggtttacgga
     1261 cgttttcaag gatgatcttc aggtttcgcc tcccgttttg caggtggctt tttacctcgt
     1321 tcagggtgaa gcctgtggtt tcggctactt cccggtacga tcgttcctca aggtaaaaca
     1381 gctcaataca tctgcgctgg tcttcgttca gccgactaat ggcttcttcc agtttgtggt
     1441 cgtcctcgct gttgttaccg gttataagat gcaaaacgct gtcgttttcc ataattccgg
     1501 cttccgattc tttcaggaga acttccttcg acttttccac cgtcttacgg tgacgcaact
     1561 gcatgaggca ttcgtttcgg gtaaccgtgt acagccagcc cttgaaagcg gaaacacttt
     1621 cgtttttcag ttctttgacc agtttctcaa aaataagcat cacggtatct tcggcttcat
     1681 cttcgtttcc gagatattta aggcatacgg cgaaaatcag gtgcgtgtac cgctcataaa
     1741 gctgtcccag gtattcggta tcacccgttc gggcatacct taccgccagt tgctcatcgc
     1801 tcagcgattg caattccttt ttcttatccc ggtaatttcc gaagaggtta aaggccacgg
     1861 cctgcaaggt ttatacaaag ttaagaaatt ggatgtctca atccggtttg taaatcacac
     1921 atttataaaa atgaactcaa aatatttcaa agattgtttc ccggtttatg gaatttcctt
     1981 acccggtgca tcctatggta aaatgcgggc ccattttaat taacctgtta acaccaaaga
     2041 ctatggaaac caagaaatca aaccgcgccg accttgagaa caagaaattg atttttctgc
     2101 agatcggtct tatcgtttcg ctgggaatag ccctggcagc ttttgaatgg gaaagccctg
     2161 accgggttaa aattgactat gaaggagtac tgggtattcc tcttgatcag gacctgacgt
     2221 ccatccaaac cacccatccg gttcttccgc ctcctgtcat gataaagccg gcaacaatct
     2281 tcaaccttgt tgataacaag acggaggttg aagacttaac accgcctgat ttcggagata
     2341 atccggaaga tatcgctcct gcctatacgc ctgttgcaaa accggttctg caggatgaga
     2401 agcctgattc cggtgaagaa gtctttatca gcgccgaaaa aatgccggaa ttccccggag
     2461 gggaaagaga aatgctccga ttcataggcg accatatcag ctatccgctg gcggcccggg
     2521 aaacgggaat ccagggaacc gtatatgtga attttatcat tgaaaaggac ggaagcgttt
     2581 cacaggttaa ggtactgagg ggcatcgggt acggctgcga tgaagaagcc atgcgcgtta
     2641 tcagcctcat gccgcgctgg tcgcccggga tgcagcgcac cataccggtt cgcgtcagtt
     2701 ttaacatgcc gatattattc aagcttaagg attcctgaga aaccacttta tcttaaaaac
     2761 aaacgaaaat gaaaacacgt ctgacaatag gtatcgcttc gggttttatt cccgtgctga
     2821 tactttcggc tgtcaccatg atcttcagcc agaataccgg aaggatgtat cctcctgtca
     2881 aaccaactat tcctgcagag gaaccggaca gccaggataa aacgctgtcg ccctatttct
     2941 ttgtcaaatc cgatgatccc gccctggacc agcttccgct gaaagccact tcagccagcg
     3001 taaacattgc cggggtaatt gccgatgtag ccgtaaccca ggaatatgcc aatgaaggga
     3061 agcgccctat agaagccgtt tatactttcc cggcatcaac acgggcggct gtttacagca
     3121 tgaaaatgac gatcggcgaa agaaccattg tagcccggat cgccgaacgc gaaaaggcgc
     3181 ggaaggacta tgaagaagcg caacggcagg gaaaaagcgc ttcgctgctc gaacagcagc
     3241 gtcccaatgt attccggatg aacgtggcca atatcatgcc cggcgaccac atcaaagtgg
     3301 agatgaaata taccgaattg ctcattcctg aggatggcgt gtatgaattc atatatccca
     3361 cggtggtcgg accgcgatat tcaaacaaac cggaggcgct ggcttcggcc gatgaaaaat
     3421 gggtggccaa tccgtacacc catgaaggag agaagccatt ttacagcttc aacatccaaa
     3481 cttccatagc ggcagggctt cctgtcaccg aaattgtatg cccttcgcac aggacaaaca
     3541 taaccttcga cggcccctcg acggcttccg tgctgctcga tccttccgaa aaatcgggag
     3601 gcaaccgcga ttacatactg agataccgcc tttcgggaaa cagcatcgaa acaggactgc
     3661 tgctgtaccc gggggaggat gaaaattttt tcctggccat gatccagccg ccgaaacagg
     3721 tgaagcaggc cgatatccct ccgcgggagt atgtttttat tatagacgtg tcaggatcga
     3781 tgaacggata cccgatcgat atctcgaaga aactgatgca cgacctgacc ggtagcctgc
     3841 gtacgtccga caggttcaac gtcatcctct ttgccggttg cagcagcctg ttttcagagc
     3901 aatcggtgcc ggcaaccccc gaaaacctga agagggccat tgcgtttatc gacagggaac
     3961 aaggaagcgg aggcacggaa ctgctgccgg ctttgcgtcg cgcccttgcc ttgaagggga
     4021 ccgaaagcta ttcccggacc tttgtcatcg ccaccgacgg gtatgtggat gtggaacgcg
     4081 aagcctttga ccttatcagg aaaaatctcg ataaagccaa tttcttctcc ttcgggatcg
     4141 ggacttcggt aaaccgttac ctcatcgagg ggatggccca tgccggttca ggaatgccgt
     4201 ttgtgatcac caaacccgac gacgcttcgg tacaggccga acggttcagg caatatatcg
     4261 gcaatccggt acttagccat atcaaggcca ccttcaaagg tttcgaggtt tacgatgttg
     4321 aaccgtcgtc cgttcccgat gtttttgccg aacgtccggt tatcattttc gggaaatacc
     4381 gcggcattcc cggtggaacc atcgaaatag acggaaccgg cggccaggga aaatttgagc
     4441 gtaaggtgaa tgttgccgga gcgaaaccat cggcttccaa cagtgccctt cgttacctct
     4501 gggccaggga gcggatccgc aacctcgacg acctggcagc agtgggaagt gcagaggagc
     4561 aaaaagaggc ggtaacgatg ctcggactga aatacaacct gcttacccgg ttcacctcct
     4621 ttgtggcgat cgacagcgag gtacgcaaca aagagggtga tgcaacaacc attaatcagc
     4681 ccttgccttt gccggagggc gtcagcgatt atgcagtcag cggaattacg ggttctgcgg
     4741 cccctatgat gatgaaatct tctccggaca gaatggcttg tgaagaggtt accattaatg
     4801 aaaaggaaga aaaggagaat gaagtttttg tagttgccga aaccatgccg gaattccccg
     4861 gaggtgaaaa ggccatgaaa aagtttatag ccgataacct tatttatccg cccctggcac
     4921 aagaaagagg aataaaaggt aaggtgtatc ttagttttct cgtcgagccc gacggaacaa
     4981 ttacaaatat cagagtactt cgtgggctgg gaggaggctg tgatgaggaa gctgtgagag
     5041 ttgttgaaat gatgcctccc tggtcgccgg ggatgcagcg tgccaggccg gttgcagttc
     5101 agtataacct cgttgttgaa tttaaatgaa aaagtgcctg aattgacttg aaaagtccgg
     5161 cgcgggagcg ccggcttttt tgtttgaagc acaaacatcc ccccaacctc cctcaataga
     5221 aatcctgcat tcaccttccc gtaaagtcta tgactcaaaa cattgatttt tgagccggtc
     5281 actacgttta tgaaaaggta tcaggatgtc taattatact ctttctaaat gtattgcata
     5341 agaattccgg gttactaaaa aaattaattt tgttccgttt ttttaagtgt ttcacgcagc
     5401 aacataactg cttatatctt cctgttatga acaaaatcga aatccacccg attcttgaag
     5461 tacctgtaaa cgaaaaagtt acatttcaat ttgaaggtaa acccgtcacc ggtgaaaaag
     5521 gtttcaccat agcggctgca ttgcaccagg ccggttaccc ggtacacagc cacagtctca
     5581 caaaccgtga acgaagcctg gagtgcggca ttggtaaatg cggtgcctgc gaaatgctgg
     5641 ttgacggaca gattcgtcgt atctgtatca caaaagtcga cggagtgaaa gaggtgatgg
     5701 aaattccccg cgattacacg cccgaaatca gggagcgcga cgcccaaaaa cccataaaca
     5761 tatacaaaac caccgtcgcc attatcggcg ccgggcctgc cggactggcc tgtcgcgaag
     5821 aattgcttaa aaaaggaatt gataacatcg ttatcgacaa caacgataaa atcggcggtc
     5881 agtttctcat gcagacgcac cagttcttct tcttcgagaa agaaaagaaa ttcggcggaa
     5941 tgcgtgggtt cgacattgcc aaaacgctgg ccggcgacag ccacgaaggt attttcctga
     6001 acagcacggt gtgggatgtg ctcgaaggca aacgcatcgc ggtgaaaaat atccgtaccg
     6061 aagaaatcta ttatgtggat gctgaataca tggttgtggc aaccggcgcg gttcctttta
     6121 tgcccacctt cgaaaacgac gacctgccag gcgtgtacac cgctgccgtc gtccagaaga
     6181 tgatgaacaa tgaattcacc ctcctcggta aaaatgtact gacggtaggc gccgggaaca
     6241 tcggttacct cacatcatac cagttgatgc aggccggagc ccacgtaaaa gcgatcatcg
     6301 aagcaatgcc cggcgaaggc ggtttcccgg ttcaggcaaa tcgtgtccgc cggctcggaa
     6361 ttcccgttct cacctcgcgt atcctcctga aagccattcc caacgatgat aacaccggaa
     6421 ttgtcggcgc cgtaattgcc gattgcaaaa actttgaacc ggttgccggt accgaaaagg
     6481 tcattgaagg gatcgatgcc atcaatattt gcaccggcct tgtttcggac gaccagctca
     6541 tcatcaaggg aaatgaaatt ttcggaagga attgttacgg cgccggcgat gccatccgca
     6601 ttggtgaagg cacgagcgca gtggtacgcg ggaagcaggt tgccatggag atattgcagg
     6661 agatgggcga aaacatcaat tatgatgaat acctcaccgt ttccaaagaa tatatcgatt
     6721 cgcagcaaca cccgatcagg gtcattcagg aaccgttcaa gccttcggcc gaacgtttca
     6781 atgaaaagcc gtttgtacaa atcgactgcc tgtacgggtt tgcctgcaac ccctgcgaat
     6841 ttgcctgtcc ccacggagcc attaccaaga cttccacgag tacggttccg cacattgatt
     6901 tcggcaaatg catcggatgt atggaatgtg tacatcagtg ccccggtttg gctattttcg
     6961 ggtatgcccc aaaaaaggac tggatattcc tgcctatcga atactttgta gaagaaaacg
     7021 aagaagtgtt cctggtcgat aatcagggca aaaagctcgg aaccggcgtt atcgaaaaag
     7081 tgctgaaaaa acccaacaaa accaatgtcg ccagggtgaa agcccatgat attcatggcg
     7141 aagcgctgat gcaggtacga ggttttattc ttcgcaaaaa ctatcctgag ccggtagaaa
     7201 tggcgccgac ttcgtttgaa tcggaacatg agatgtatgt ttgccattgc gatgatgtga
     7261 ctttgggcga aatattgaat actatcggcg accgtaagtt tatttcggtg gatgaaatca
     7321 agcacaccac ccgtctgggg atgggtgcct gccggggcaa gcgttgcatt cgccggctca
     7381 aacaggtttt gcgcagcaag ggtatcagca ttgtaggcga tgccacgccc cgcgggcctt
     7441 taagcaacca ggtgtcgatg ggagaattgt tcccgaagga agtacgcgaa aatattatca
     7501 ccaacggtaa aaacgcacgg cggcgggttg atgtccaggc tgttattgcc gggggcggta
     7561 tggccggaag cgctttgttc cgctatatgg ccgaagccgg tttaaaacct gccctgatca
     7621 attacgggcg cggatcatcc tggcgtaata tcgccggggg ccgtccggca ttcagtatcc
     7681 ctgaaattgc cgatatcgcc cgtcataact acgatatttt cagggaattg caaactattc
     7741 gcgatgttaa tttccggtcg acccgctatg tgacttttgc ccatgacgaa gccacctgca
     7801 aagcgctcga agattcccgt tcctggtcgg atgcttacat ggtcgatccc aaagattttg
     7861 ccaaagaaat cgctcccaat atcaatccaa gcctcggcga catctatccc gcggcgctca
     7921 tcacccgcga ttgctggcag gcaacgccgg gccgtgtgat tgacctcgtt cgaaggatcg
     7981 ggattgaaaa aggcggaaag gtttgggaag actgccagct tatcgatgtc aggaaagaaa
     8041 acggggaata tgttgtgctt gcccgtgatc acgaaaagca ttatatcgaa ttccatactc
     8101 cccattttgt caattcactg ggaccgggag ccgataaatt cgccaaacaa ttaggcatta
     8161 acacgggctt ctatccggtg aaacaccagg cctttattac ccgacgcctt ccgaatatgg
     8221 ggattgacgg acaaccgctc gatatgctca tcgacaggcg caggtacaaa ggattttcag
     8281 ccgtttacgg tcagcagctt ggcgaaacag gccagatcat cggctgtgca tcgcccctgg
     8341 tagaacctat ggaagttgat aaaaacctta aagtcaactc aaaggatttc cttgaaatag
     8401 tttcggaaat ttttgttcaa tggctgccgc aattgtcgtc ggtaggattc caggctgttt
     8461 gggccgggta ctatgttgaa ccccgtatga tcgtggatgt cgatgccggt ttactcgtcg
     8521 gcttaagcgg acatggcttc atgctgggtc aatacctggc taaaatgtac accgataaaa
     8581 tgttggggaa accggttccg gattatctcg aaaggctttc ggtgaacggc gacggcttaa
     8641 ctgaaaaagc tttcaaatag actcatcgct gaggattaca aaaagaaaaa gggcggtccg
     8701 aaaaccgccc ttattctttt tccggatcac gacttccgga ataaccgttt aaagaatgta
     8761 tctttctcgg tatcggcatc atcggaataa tagccgtaac cataaccata gccataaccg
     8821 tagccgtaac cataaccgta gccatagccg taaccatagc cataaccgta cccgtaataa
     8881 cccgattttt caggcttcac gtcgttcatc aacaccacca tgttcgggat attgcggttt
     8941 tcgatgtcgg cggcgatgga accaaaaact tttttattgg ttaagttctg gcgaaccacc
     9001 atgatcttgg catcaacaaa cttcatcagc aggaaagcat ccgttaccag gccgaaaggc
     9061 ggggtatcaa ggatgataaa gtcgtacatc tctttaagct tttccatcat ttcagccgtc
     9121 tttggcgaag cgataagctc ggcagggttc ggtggcaccg gtcccgacat aatgacatcg
     9181 aggttatcga aaggcgactt ctgaataatc tcttccaggc tgcatttacc gatgagatat
     9241 gacgtgatac cggccgtgtt ggttaaaccg aagtcctggt aaattttggg tttgcggagg
     9301 tcgaacccca ttagcagggt cttgcgctcg tactgggcat aaacagccgc caggttcatg
     9361 gaaatgaatg tttttccctc gctgactgac gttgaagata ccaggatggt tatcttgtct
     9421 ttcccctggg taatatattg caggttggta cgcaaagccc tgaacgactc agcaatggag
     9481 gattttggcg aagtggccac cactacggtg ctttcgcgcc ggttatgcag cacatgcccg
     9541 atcagcggca gtttggtggc gttttcaatg tcgtgtttat ccacaatttt atcgttcaga
     9601 aaatcctttc ccagtacata caggataggt aaaaccaatc ccaataccag ggcaatggta
     9661 taattgagcg atgttttcgg aaataccttt tctttaactt catcgttcgc catgtcaaca
     9721 gattcgttat ccggcgtgtt gcttgcacgg gtaatggccg attcggaacg tttctggagc
     9781 aggaaggtgt aaatggcgtc gttgagttta tacttgcggg taatcccgat gagttcgcgc
     9841 tgcgtggcgg gcaactgcct gaactgtccc gagtatttgc tgatgcggtc gttgatatcg
     9901 ctgacggcta tattcgatgt gttgacaata ttcctgatat tctcggtcag cgattttttg
     9961 gtgttgttga ttttctgatc aagcgccatg atcagcggat taccttcttt agtattgatg
    10021 agcctttcag cccgttcggc atacaaagcg gtgagttcgc cgatcagttt tgtcagcccg
    10081 ggatcattga tgcccatcga agtaggtact accagactgt cgagattctg ctgcttggcg
    10141 gcgagataat tctggaggta tttgtaatat ttggcttcga caagcaattg ggctttctgg
    10201 gtctcaagat cttttacatg ggtaaaagcc tcttgtgtct gcgcatcaaa gtccatgatc
    10261 tcgttcaccg tcttgaagtt ctgaagacgg gtttcgttat ggcttaatga gtcgccgatt
    10321 tccaaaagct gcgtatcaat gaagttgatg gtattgatgg caatctggtt cttcttgtca
    10381 aggccctgct gaatatactc ccgggtaagc tggttcagga aatcgaccgc tttctgagat
    10441 gtattgcctt tcagcgaaat ttcgacgatg gaggcatcct tgttgatggg ttccacttca
    10501 tagccggaga aggccgaaat cagggaattg agcgagttga aagtgaaaaa gtactgctgc
    10561 cccaggtgtt tctgttcctt aaatttattg ctgaggcgaa ggaagaaact aaattccggg
    10621 gtggttatct gttcgccaaa ccgggctttt ttcgaaaatt tgaccaatcc ttctttcttg
    10681 ccggcatcag tgtaattgtt gtatttgtat agttctacat cctgtccttc gcatgatagt
    10741 tcatactcgt ctttcgaaag aagcgtaaca taaaatttca tgttgctgac tggctgcaca
    10801 tgtcccgtat caaattcgac gaggaacggc gattcggtgt atatctcgtt ggtgataaaa
    10861 ttatcttccc tggagtaggt aacttcgaag tgcaatgctt ttaatgtcct ggttttcagc
    10921 gaacgtgagt tcagaatgcc gatctggttc tggagcttct gggtattcgt tatggacccg
    10981 aagcccagca aagcctgcgc atcgcttatt ccgccgcctt catccttcac gagtacagtt
    11041 gcctttacct tatattcagg cctggtgtac ttattaaaca ggaaggcaat aaggagcgac
    11101 acgaaaatag tgatcagaaa cagataccag taattataaa agcggaagaa aatcgcttta
    11161 tagtcaatgg attgttcctg gaaatcaggt tccggaaaat tctgaatcgg gttgctcatg
    11221 ggtgttattt aaagaatgta agtaccagga aagcaagcga aatggtagaa tagatcaggc
    11281 tgtaaggaaa ggtttcaaaa gcgaactgct ttccttttaa aggttcaaca tatacgatat
    11341 cgttgggcct caggtaaaaa tattctgagt ttaatatatc ttttctggtc aggtcgaagg
    11401 tcaccacttt cgtttcctgt ttgtcctgcc ggatgatctg cactttctgc ctgttggcga
    11461 aggtattgag gtcgcctgcc atggccaggg cttcaaatat attgatccgg tcgcggttaa
    11521 tggtaaactg ccccgggtgc ccgacttcac ccaacattga aatcttgaag ctgaccagtt
    11581 tcacaacaac ggtggcttct ttgatgtatt ccgtcaattc tttatggagt tcctgctcca
    11641 tctgccccgt agtaagcccg gctgccctga tgaaaccggc caccgggaaa ttgatatatc
    11701 ccgaatcgtt aaccatgtaa ctggtcaggt aggtatactg atcggtatag gaacctccca
    11761 tataggaaga accggaaatg ctgttgaaaa gggtattgct cttttcgtca agggtaaata
    11821 tccgaatgta aaggtaatcg cccggctgta aaaggtatgc ggctgacaca ttgcggaaaa
    11881 cccggaccgt atcggcgccc tcagcagtct gaagataacg tatctttttc tgcggaacgc
    11941 atgaaaacag cgccaggctc attactgcaa gcagtaaagc tggtctgaca aatcgggaga
    12001 tatttttcat aaggagatat tagccctgaa atctattaat aattacagat tagtatgcaa
    12061 aaatatgtaa ttctgttctc agatttacag cctgcggcat ttcatgaaaa aatgacggtt
    12121 aaaaacgaaa attaaggcag ccggaaacga cttgaaggcc aaggatataa ccgccctgaa
    12181 gcgtatctta agctttggtt gcgcgcctgc agccagtaac gtagcctagt ttcacgcggt
    12241 tggtcctggc agtggccgga gagccggcat gatctgaccg cgaattgctc agatcaggca
    12301 cggattacca cgaaaacgct tcgcgaaatt tcgtgttgct caggcgccct aggcatttgc
    12361 aggggcaaag actaaaaata gtagcctttc ccgtcgcaat cggtgcaatg ctcatttaac
    12421 cctgcacaac gacacgggcg ggcgccttcg ggaatatcgg ggacaggatg cgatttcctg
    12481 tcggtaaccg aatccagtcc cagccaatga tcccagccgc gccatccgcg gttctgatag
    12541 gcaatatcag gataggcata aagatgttgc gggcgtttgg cctgactgta atttttccag
    12601 tcacggctcg atttcaggtt caacgatatc acaaacctgc gggttgtttc ataatctttg
    12661 aattccgtat tcaatcccag ccagtcgttc catcctttcc atcccgaatt tttatattcg
    12721 tattgcggga gcccgggcaa tacgaggcta taagccttat attcaggatg atcagccgac
    12781 aggtattcca accacccggc gctggatttc gaaaaacgag tgatcctgtg atatgaacat
    12841 aacgactttg gaagcgcttg tcagcgcagc aatgaataga atatcagata tttatggttc
    12901 ttgtttattt cagtggtaaa ggctgattaa gctgtcgatc gtggtttcgc cgatgtggtc
    12961 gcctttgaaa atcaatgtgg tatctttaaa aatgtaatca tcatagcccc atttattttg
    13021 tttcgtgatc tcgataccca ggtctttcat gaatttctgc cttgcgggat tcaatttatg
    13081 gccataatat ttgtcttcca ggacatagaa agtatgatgc gacgcagtat ccgccagttt
    13141 ttgtttaatg tttcttacac cataacattc agaaataaat aaaagatcaa ggccttttaa
    13201 ggtgtatttg ccggcaaggt tgaagaagta atcaatatgg gtgcaataat ctgaggtaca
    13261 gtaaggcctc cataaatgta tccatgtgta atggttgtac ttcatccgtt gaagcatttg
    13321 aggcgtattg atttcataga ggaataaatc tcctgtattc cctgatccgg attcagaaac
    13381 agcgctatat ggtttgaaca tggatttatc tgtctcctgc aacatcctgt aatcattctc
    13441 gttgatcatc accatgcatg attgtaagaa cattacaacc agtatcacag tcgtttttct
    13501 cattttcata ttatttaaag gttccgtaaa aagttctata gcctgttctg ctcccttttt
    13561 ttcgccggct aaaatcgcag ggactcttct ttagcatatt gaaccaatcg atgagcaaga
    13621 ctgaagcgga gatgctttcc cacatacgca gtcaaacaat gcacatggct gtgtacaaaa
    13681 cctgcgcaga ccattgttct gtcaaaccaa taaacaattt gtccgttttt gggtcatttc
    13741 ttaatacagg ttttatttat tttggttcgt acctggttat tttgcgttta ccttaaatct
    13801 tccggggagg cttttttgtg ttggggaggc tgaccccggg tattcaaagt tctatacggc
    13861 aaaaggcaat cgcttcaata atataatccg gtaaatagtc accctgccgg cgttttggta
    13921 cttgttgtat ggtaacttta atgtttttgt ttatgaacct tattgtataa atggtatcaa
    13981 tttcctcaca ataacttcct gaacgattca aaatcagttt acttaaccaa agatagtata
    14041 aaaattgaaa aaagcaacag agcggttaaa gaaaatttga aaagtcaaaa aaaaacagat
    14101 aagcggtttt aaaactgtca actgaaagcc tcagacaaac agttggttct tcccttaaag
    14161 cgctacagag agaataaaat tgtcagatat gtgatgccgg tccggagacg gccgtcttca
    14221 ggatagcgga gcaggtacgg ttaaaattga agcgaacaga cgatagcggc ggcgaggact
    14281 cttacagcca tatcatcagg ccgtacctgc atttttcaaa tcccgtatag ctttattctt
    14341 ttcaaccgca agctgaacat ctcagccgcc ccgggaatgg ataagaaaat tattcacacc
    14401 acgccttcac ctcttcaatc ttcaaagccg gaatctccag tttcagcttt ttgcgcaaac
    14461 cgctgaggtc gttgagtaat ttattgggat tggcagcttt tacgtcggct gccgttttaa
    14521 gcccggcttt acgcaccacc ggaacccaat ccgccggtat accgagtgcc aggaattcat
    14581 tgtcgttgtc cacaggcgcc gcgatctttt cggggcgcat ctgcgggaac aacagcacat
    14641 cctgaatcga agcctggttg gtcatgagca tggccaggcg gtcgatgccg atgccgattc
    14701 ccgacatggg cggcatgccg tattccatgg ccctcaggaa gtcgtaatcg ataaacatgg
    14761 cctcgtcgtc gccgcgttcc atgagcctta cctgctcttc gaaacgttcg cgttggtcga
    14821 tggggtcgtt tagctcgctg taagcattgg cgatttcctt gccgttcacc atcagttcga
    14881 aacgttctac cagcccgggt ttgctgcggt gtttcttggt gagggggctc atctccacgg
    14941 gataatcgat gatgaaggtg ggctgatcgt agtagccttc acatttctca ccgaaaatgg
    15001 cgtctatcat cttgcctttg cccatggttt cgttgatctc gacttcgagt ttaccgcaca
    15061 cctcgcgtaa agcgttttcg tccatgccgt aaacatccat gccggtatgt tccttaatgg
    15121 catcgaggat gccgattctc cggaacggcg ctttgaaact gatcacttta tcgccgatgg
    15181 ccacgtcggt ggtgccgtgc aattccatag caaccttttc tagcatcttc tctgtttgct
    15241 ccatcatcca gaaatagtcc ttgtaagcca cgtaaaactc gaggatggtg aactcggggt
    15301 tgtgggtgcg gtccatacct tcgttgcgga aatcgcgtgc gaattcgtaa acccagtcga
    15361 aaccgccgac gatcagccgc ttgaggtata attcgttggc aatgcggagg tacaggggaa
    15421 tatcgagggc gttatggtgc gtaatgaaag gccttgccgc cgcaccgccg gggatgcttt
    15481 gcaggaccgg cgtatccacc tcgagggcgc cctggctgtt gagaaactcg cggatggcat
    15541 tgatcatcct ggtgcgtttc ataaaaacct ctttcaccga agggttcacc acgaggtcga
    15601 catagcgctg acggtacttg aactcggcat ccgtcacggc gtcgaaggtc tggccgtcgg
    15661 cttcctttac tatgggtaac ggcttcagag atttactgag gattttaaat tctttcacgt
    15721 ggatggagat ttcacccatc tgcgtgacga acacatagcc gcggaccccg atgatatcgc
    15781 cgatatcggt aagtctttta aaaacggtgt tgtacagggt tttatcctct ccggggcata
    15841 tttcgtcgcg tttcacgtaa agctggatgc gtccggccga atcctgaagg tcgacaaacg
    15901 atgcagcgcc catgatacga cgcgtcatga tgcgtccggc aatataaata tcctggtaaa
    15961 gagcaggatc gccggggtaa ttccccagga tttcggcaga ggtgacattt accggaaaaa
    16021 gctccggggg ataaggattg ataccgagtt tgtaaagttc ctgcagcgaa ttgcgcctga
    16081 tttgttcctg ttcactaagt tcgaaagcca ttgagaaaat ttttgcaaag atactgaaat
    16141 gtaaaaaatt gcgggaaatg tgatctgaca cgttattaaa acccgtcgga tccgctatgg
    16201 tcataagttt caaaacatga gcaatgacat aagttgctat ttttggacaa atttatcagg
    16261 caaatgaaat tttcacatct tactgattcc tttaaaacta catacttccg gtggatcgta
    16321 atgatcatgc tgtttatacc gctgctgttc atcagcaatc gccggagcca cgactggggc
    16381 gatgactttg cccagtatat tcaccaggca aagaacatcg tgcagggcat cccgcagtcg
    16441 gaaacggggt tcgtttacag ccaggaaaac ttcattgggc cgaaagctta ccccgtcggt
    16501 ttccccttgt tgctgtcgcc ggtttacgcg gttttcggca acaacatgaa agcatttacc
    16561 acttacatat cggtgtttta tttcatactt gccctgttgc tggtgctttt cttccggaag
    16621 tatttttcgc tgatcccatc ggttgtcctg gcgatgatcc tgctttataa tccgcagatg
    16681 atgctgttta agcaggaagt gatgtcggac ctcccgttta cggtattact ggtcgcctcg
    16741 atgttgcttt atatgaattt caggaaaaat aacacggtga aaataatact tgtcctgata
    16801 gcaggattga tgatcacggt gaggccgata ggttttgttt tcctgatagc cgtgctgatc
    16861 gaccaggtgg taatgctgct gaaggaagat tgcagccggc gcagaaagtt ttacgaactg
    16921 gttgtggcga ccgtatttgt tttgcttacg tcgggcatct acctgctgct gaatgtggcg
    16981 atcttcagga taccttcggg cggcggcctg aacaactacc ttgcctactt cggaagcggt
    17041 gaaatcgtca gcaccatcct gaccaacctc cgtcattatt ccgaagtatt ccaaaacttc
    17101 tacacgccgt cgctgggcga ttacgatgga ttttcactgc tgtcaggcgt acttttcctg
    17161 gccatggcta tggtcggcct tttaaagagg tttacgaccc gaatagaatt cctcgatatt
    17221 ttcttcctga tatacctggt aatcctgctg gttttcccga acaacgcctc tgcgttcagg
    17281 cttatggtgc cggttagttt tctgctgtta ttgtatgtgg caacaggatt ccgatcgtta
    17341 tccgttcttc cgtccgtgag cgggaaactg aaagccgcga tcctgggaat tctcatactg
    17401 gccttttata tgccggggat ccgggatatt atcagcacaa aatatatcat catggccggt
    17461 ccgcaaaagg cggaatcgga acaggccttt gactatatcc gtgaaaatgt tccggagaat
    17521 gccgtcatca tgtttgtaaa gccaagagct ttgtcgctgt atgccggaag ggctggtttt
    17581 gccgatccgg cggagaccga catgacgaag ttccataaac aggtggtgga tgccggcgtg
    17641 aattatttcc tgctgaacaa tgacatcagc agtgaagcct cgaaacggta tcttcgggtg
    17701 atgaatgaac gggtcgattc tgtgtggtcg aatgagacct tcgggttata ccggctgaaa
    17761 agtttcaatc cctgaaaact tcgttcagca cgtgatgcga tttcatttcg ccgaaagccg
    17821 gaacatgcag cctgaaataa tccatcagcc tatcgagcaa ggctttccgg acagaatagg
    17881 taaaatcctg cggtaaagcc cggccgtccg gaaagccgat caattcgtgc cagaaacggc
    17941 tgagcggctc agccaggtaa tagggatgat cgggcggatg atcccggaaa tacgcttcct
    18001 gaagatcaaa aaaattgttt gccggcgtat aattgtcgtg aggcccgaat cccaggtgat
    18061 gtgccagttg catggcaaat accaggtgga atccggcaac ggaaatatcg gccgaatcga
    18121 ggtcgagcac ggtccggtaa aggaaattgt ataattgctg atcaggcatt tccccctgta
    18181 ttgatctgta caccaattcg ttgataaaaa cggcgacagc catcttcctg atatcgctgg
    18241 caatatggtt aagctgaacg tggcaggtga catcccgggc aacgctcaac cccgtatttt
    18301 ctttgcgata ggccacaatt tcgaccagcg acaacggccc gaaaaggttg cgtttgatct
    18361 tcgacctagc tttgcgcaca ccctttacca ggtacgactg cataccgaac tgttcggtaa
    18421 aaaccttaac gatgacgctt gtttcggaat aatcgacggt atgaaggacg atccctttgg
    18481 tgacgataag cataagtccg tgccggattt gatacaaagg tattgaatca gcataaattc
    18541 aggatcaaaa gctttgatcc gttgtattcc ggcttgctgc acatgattca gcgaaaacag
    18601 gtggcggact tgccttggtc ggtgcgcctt tctcattaaa gcgcatcacc ccataaatta
    18661 ttgaggagtg ccgttgcccc ctgaagcaga gaaatcagtg cacaaaaaga atcttggcca
    18721 cctgcgtctg gctgccgtcg ggattggaga tgaaaaccag gtaaactccc gtgctggcac
    18781 gccgtccgct gaagtttttc ccatcccata tcacctgtcc gccttcggcc tgcgtttccc
    18841 agatcaggct gccgcttatg tcggtgatct tgatattgga atctttcact aatcctttga
    18901 tggcaatatt tcccgtatat gattccttca cggggttcgg ataaacgatc accgaattca
    18961 gcgatgaatc gggagctatc ttgtaatccc tgtatgaaac gatgccctgg ctggtgccga
    19021 aaaacacttc gccatcggca gcaatcctga tgtttgtaat cgtattggat aagagcgggc
    19081 tgtcttcagc cgtaaaatga tggatttcct ttgatccgtc ggcggacatc aggaatacgc
    19141 cggctttccc ggtgccgatc catttctcat tgtcgccgtt gacggtaatg gcggttatcg
    19201 attccgtttc caggaggtta taaaggtatc cgtccacttc aacctgaacc acctgggcat
    19261 cgtagttgcc cccgttaaag acgttttcgg gtgaataaat cacggcaact ccctggtcgg
    19321 agccaagcca gagctgcccg tcgttatcca ccgccatgct gaggattgcc cccgttcccg
    19381 gcaaggctcc gttaccggcg gtaccggtca ggaaaatgcc ctggtcatcg gcagggttgt
    19441 caattgtacc gttatcatta aaaaccacga gcgtatggtc gcgcatctgc agccatttct
    19501 gtccggcttt atcaataaca attccgccgg tataataagt actggagccg ggggcgggat
    19561 tgaacgaccg ccaggtgcca tcggctttgc gcaccgatag gatttcgggg gcagtattgt
    19621 tcgtaaccca aaggttgtta tccttatcca aggctatgcc tccggcaaat accaggctgg
    19681 tgtcgtatac cgaaccctga agggaactgt tgcttttatc ataaatggtc acaagctgcc
    19741 cgtcgttgaa ttctatcagg ccgtaccccc aggtaccaag aaacgcatgg cggggatttt
    19801 ttttatccac agccacacat acgatatccc gcatatcttt gaggaaatcg gcgttcaggt
    19861 atgagaatga gctccagttt ccatcaatat tataatagaa acgggcgagg ttgtacaaat
    19921 tgccaaaccc gttgtcatgg cctccgggaa ccgcccacag gtcgtttccg cttacatcca
    19981 tagccgtaac cagcgcactt acaggcccgt tgatcaggat ttgcacgcca ttccactgat
    20041 cggtcatctt atataatcca ttgtcattgt cggcaatcca cactttattc tcatcatcaa
    20101 gaatggcgtc gtttggccgg atagcacccg gattataact atacatccgg tagtcgagtg
    20161 ttccgtcggg tttaaaagca tcaacagcca ggtagttgca taccagcagc ctgtcgttca
    20221 tcaccctgaa tccggccctg gtggaatgat tagcgggctg gaaatattcc cagtggcttc
    20281 cgtcgtaaac cagcatggta tctttgctga aagatgagaa atccggggtt gaattcacat
    20341 atagttttcc cccatgccag gcgatataat tatatttccc tgaaggatcc gtaattgaat
    20401 tgtcctttgt ccagttgata taatacgcca ggtttgagga agcgtctgca tgatagatac
    20461 ctgcgtcggt ggcagcataa aaatacttgt cgaaagcgaa ggtgatggca ttcacattga
    20521 tctggtctcc ttcggggcca atgtaccagg tgtcgtggat ttcctcccgg tcaatatcga
    20581 gcacaacgac gccgaacccg caggccaggt aggcataatt atcaattacc gtgatgctgt
    20641 taatggtttt attgccaagt atttgcttgc gtttgatatc cggaatattg atgacaacat
    20701 cgttcttcac caggtcgata ttggcattgg cataagcaat tatcaatgtg ttcagcgatt
    20761 tgctgaaagc gatggccgaa atatcaacat ccgataaacc ggtcacttta ttaagccgcg
    20821 ttatgctgtt atcggttttg tcgacataaa aaaggctgta aggcgtagca caataaatgc
    20881 gcgaccctcc ctggacaacc gctttacagt tattataagg aagataatcc tgccattccc
    20941 cgattcctga ggattgggcg ctcagggcgc cggcaataaa aagagagata accaggatga
    21001 atgcatgaat tttaccggaa aacatcagca tgaaattagt attgatacat gattcttaat
    21061 taactcgttt atatccggat ttattgtctg tgcaagacta aaagtatact tcagcattcc
    21121 ctgaaggatt tgtcacagtc atataatccg ttttgctgag gtttaggaat accgttttgt
    21181 ctgttcagcc tttgtgcgcg gcaaatatcc cggatatccg gcgaaaggtt tccgttggcc
    21241 gggtctttac cgggaagctg tgccggcgct ttttgtctgc ttattatttc ttttaaacat
    21301 tccatacttc attacgcagt ataacgccct gaaaccatct ctccagctta tttttttgcc
    21361 ttcctcataa gttcttccgt aataggaaat tccgacttca taaatacgga ttcctttgat
    21421 acgcgcaact ttctgggtca cttcgggctc aaagccaaaa cggttttcgt gcagggaaat
    21481 gtttttgata atatcgcttt taaacaattt gtaacaggtt tccatatcgc tgaggttgag
    21541 atttgaaaac atgttgctta tcagcgtaag gaacctgttg ccgatggtat gccagaaaaa
    21601 aagaatccgg tggggcttgc cgcccatgaa ccgcgaaccg taaacgacat cggcggcccc
    21661 gtcagttaca ggtttcagca aaatattata ttcattggga tcgtattcca ggtcggcatc
    21721 ctgtataatt ataaaatcgc ccgtagcgac acggataccc gtatgcaggg ccgccccttt
    21781 ccctttgttt acctcatgtt taaagtactg taagtccagg tccgtatgat ccgctttgta
    21841 gttcagaacg gcttcttccg tattgtcctt cgaaaaatcg ttgacaacga taacctcttt
    21901 tttaataccc tcaatcagtt caacccggcg aatacgatca aggatcagat gaatcgtttt
    21961 tccttcgtta taggccggta taataatgga aagtttttgc ataacccggg aaaataaaaa
    22021 gcaataatac gaataaaaag atatatgcgt cgacagcgtc aggatataat cacgaataag
    22081 gcttttatct tcgctgatcg gacgggttta atatgggtac taaaatggcc cgggccattt
    22141 cagatacgtt tcttcatcca cacatcactc ccgaaaaaag cggtaaaata gttttcctta
    22201 agtatggtat taattgcctg agtgaaagcg acacacaatt ttcaggaaat aattgtgacc
    22261 aaacgaagat attttgtata tcttcacacc aaaatttcag gaactgattt ccggtaccgg
    22321 aagtctaaaa ttgtcaaccg gcctatcaaa cacaacggta atgattcact attcggattt
    22381 tgcgataatt gaggggctta ggctcaacag tgatttcatt atccggtatg tttataagga
    22441 gtttttcccc accatacgct acctgataaa aaacaatgca ggccttgacg aggacgcgga
    22501 agatattttc caggaatcct tgtatatcgt cctgaagaaa atccaggaag aagattttga
    22561 attaaattcc tcgtttttga cctatttgta ttccatcagc aaaaaccttt ggttgcagcg
    22621 gctaaaaaga aaaaggctgc atgaaaagaa ccaaagcgat cttgtgcatt ttctttcatc
    22681 gcctgataca ggcgtggaag tgcatataga atacgaagaa aaataccgtg tttttattga
    22741 acagttcgaa aacctttccg aagattgtaa aaagcttctc caccttttcc tgggcaagat
    22801 gtcgctgaag gaaatcgccg atgaaatggg ctacaaatcc gaactttatg ccaaaaccaa
    22861 gaaatacctc tgcaaggagg cgcttcgcaa gctggttgaa aacgacccgg caatccgttc
    22921 gatgaaatag cagttaccgg atttctctgt tttactgata acgacttccc ggatgccggg
    22981 attccttata attaaaaaca cgcgaaactt ttcacctgat attgccggaa acaagaactt
    23041 tgcagtttga accccatttt ccgaacatga gcaattttga ggatcttttc ttttcgccgg
    23101 tcaggctcag atttcagacg cgcgaagtgt ttgtcggcga tgtgcccctg ggaagcaaca
    23161 accccgtgag gatacagtcg atgaccaata ccgataccaa cgacattgaa gctacggtta
    23221 gacagaccat gcaactggcc gacgcaggtt gcgaatatgt ccgcattaca gcccagggcg
    23281 aacgggaagc cgccaacctt gccgttatcc gaaaaacgct ccattcacgc ggttgccggg
    23341 ttccgctgat tgccgacata catttcagcc accgggccgc cgaagtggca gcatccatcg
    23401 ttgaaaaaat caggattaat ccgggcaact atgccgaccg gcgtcatgat gttcatattc
    23461 attacaccga ccgggaatac gggctggagt tggaacgcat cgccgaacgc attcatcccc
    23521 tcctgaaaat atgcaaggaa aacggcacgg ccatgcggat aggttccaat catggctctc
    23581 tcagcgaccg tatcatgagc cgttacggcg acacggccga aggcatggtt gagtcggcgc
    23641 ttgaatttgc gcgcatttgc gaagatgccg ggttccgcaa cctggtcatc tccatgaaat
    23701 cgagcaatgt acgggtgatg gtccatgcta cccgcctgct ggtcgaccgg atgatggccg
    23761 aaggcatggc tttcccgctg cacctcgggg ttaccgaggc cggcgacggc gaagacggca
    23821 ttatcaaatc ggcggcaggc atcggttcat tgctcaacga cggcatcggc gataccatcc
    23881 gtgtgtccct taccggcgac ccgctgcagg aaatcccggc agcacacgct atcgcccaac
    23941 ggtataatgt aatgaacagc cctgcctgcg ttgctttgag tcatcataaa ccggaaaacc
    24001 ggaacagcgt aggggctgat caatgctctt ttaatccttt cagtttcagg aaacgggaaa
    24061 ccattgcaac cggcaatatc ggcgggaaca ggcccgttca ggtgcttgcc ggaaaaaacg
    24121 ggatcctcag cctggtggat gaatacgact ccgctacggg cgctgtgacg gaacaggcat
    24181 tttcaggcct gattgttcag gaggaagagg tcagggcatt ccggtccaaa attctgagcc
    24241 tcccggtttc cgaccagcgc cccgtcgtcc tgacaaagga atatcagacc aacgacctta
    24301 aaagctttac gatcgacagc gctatcgatt ttggttccct gctgattgaa gggatcggcg
    24361 aagccatctg gccggtagcg cccaacatac ccggcgaaga aacaacgcgc atggctttcg
    24421 gaatcctcca ggccaccagg acgcgtatta cacgtactga attcatttcc tgtccatcct
    24481 gcggaagaac acttttcgat atcgcgtcaa ccctgcaaca ggtcaaacag aaaaccggac
    24541 atctgaaagg cctgaaaatt gccgtgatgg gttgcatcgt aaacggcccg ggcgaaatgg
    24601 ctgacgccga ttacggttac gtgggagccg gcaggggcgc cattacgctg tataaaggaa
    24661 aggaagtcgt caaacgaaac atccccgaaa cggaggctct caacatgctg ataaacctca
    24721 tcaaagagtc gggcgactgg aaagaaccgg agaattcctg atgtagccgg gcaaccatgg
    24781 ttatgatttt taactaccgg ccgaagaaat cagaagtatt tatttccccg taatgatatg
    24841 aagactttgc tttttgatgt tgaactcaaa aggcgaatgc cccaacgatt cgccgtcgac
    24901 ttcgagatat aaaggcggat cggattcaat actgatgaat gccgatttaa acgaactaac
    24961 ttccgggagc ttggtaaaag aaccgtccaa caggttcagc acattccgga tagccttgaa
    25021 tttaccgata ggactgatga tgataaggtc gaataatcca tcgtcgggaa cggcaaaagg
    25081 cgattgtttc attcctccgc cattgaactg gcatattccc acgctcatgc tgaacacgcc
    25141 ggaacggatt tcccggtcat caagggtaat ttgggtccgg gttactttaa aggaaaacag
    25201 gcttgaaaag atgtttacaa aataggataa tggattgcct ttgccagctt ccttctgtat
    25261 gtttgtcttc tttgcgacca gtgcgtcgaa acccatcccg gccatgttgg caaaatgtcg
    25321 tttcttttgc agcccttcgc tcgaaatata ttcaactacc ccgatatcct gaaggaacac
    25381 tttccccctg acaatcaggt ttatggcatc tttataattg aggggaattc cgaacatgcg
    25441 gcaccagtcg ttgccggtac ccaccgggat catggccagg gtaatatccc tgggattggc
    25501 atgatcctgg gtgaagatgc cgtttactac ttcgttgagc gtgccgtcac cgccaaccac
    25561 gataattttt ctgaaaccat tctcgatata ccttcgggtc agtttcatgg catgtcccct
    25621 gtgttcggta aagatattgg tatatccaat accggcctcc tccaacaaat gcgctatcga
    25681 aagccagtcg tgctggcctt tccgtttgcc ggcattcgga ttaacaatta caaaccactc
    25741 aacatccatc ggtcattgtt tgaaatcctg ccgggttatc cggcacttta caggaaaata
    25801 tagtattccc gggcgataaa agtaaaaaaa catcggttat cagaaataaa aaaaaggttg
    25861 cattctcagc atgccggaat cttactgaca gaataacaat ccggaacaac cataggatca
    25921 ggcaggtccg gatgatatag cctgcgggtg acagttcagg taatatggat tcagattccc
    25981 aggctgcgct gagttatatg tcaatgttca gacaactttc tgcctccatt ttccatattt
    26041 caggtagaca aaggacagta caccgacgaa aagagcatac acccattcgg ctgtccaggc
    26101 tacttcgaca ggttgatgaa gcctgatggc catcagccag gtgtacccta cataaaaggt
    26161 gagggtgatg atttcgatga acaggctgac acttgtattg gccgttccca aaacggcatt
    26221 gaacaaaacg cttaccagcg aaaagaagaa caaggcaacg agaacgatgt aaaaggtggg
    26281 aatggcagcc ttaatgagtc cggcatcgtt ggtaaaaatt cccaggattg gtacaggcaa
    26341 gaaggcggca acagaaacaa cggccagcaa catgacaacg ctgaaccgca tgattttata
    26401 ggtaaccggt aacacctgcc cgggctttcc ttcgccaata gcgttactta ccaatgtatt
    26461 ggtaaccgaa caaagcgccc agccgggaat cattaggaac atatataccg accgggcaat
    26521 gtttgaaacc gccagcggcc gttcgcccat tttttcgatg atgaggaaaa atacgaacca
    26581 tattccgagc gacagcacaa actgtatcat cacaaaaatg gaaatgccca gcgtattccg
    26641 gatgatagcc ggatcaggtt taaggaacct gaataaaccg taaggtttca ccctttcgga
    26701 cctgttggtg gcgatgatga aaaaaacaag cgccaccgcc tccgaaattg ccgaagcaat
    26761 ggcggccccg gtgagtccca tttcggggaa accggctttc ccgaaaatga gcaggtaatc
    26821 aagaacaata tttaccacag ccatgatggc cgagctgatg ctgaggtacc gtgtataagt
    26881 gataccggta taaaatgacc ggtacaacaa tccgatcatc gcaaaaaaca atccgaagac
    26941 cctgtattga agataggttg atgcagcttc aaaaattgcc gtcgacgaaa caatgggttt
    27001 caggatgaca gaccccagga aatatataaa aacagtaaca acgatggagg taaccgccag
    27061 aaaataaaag ctgttgtcga aaatccttcc gatctccccg aaatttttct ccccaaaacg
    27121 gcgggcgatc aaaatctgta ctcctgtaac aaaaccgtta ccaatcataa acaggataat
    27181 atagaacagc cccccgatag cagagccgcc cagcgctact tcgctcactc tccccaggaa
    27241 agcggtatcg gttacgccga caatattctg ggccagcagg ctgatcatga tgggccatgc
    27301 tatttcccaa atccttttta acgaaggggt atcagaattc acaggactgg tgtttggttt
    27361 tatccggaaa ttgaataaat ccggcacaaa tcatcattta cttttacagg ataaggcttt
    27421 atttaccgat cgaaaaatca tcccagtcaa gggccacctt aaaaccttcc cggtattcgt
    27481 gcagtttttc atacgaaagt tggaccgttt ccacatcctc cacatcggga ggtatttcaa
    27541 ttatgggttt tccacggtaa tcgagtactg ccgaatctcc ctggtgaagg gtgcccttgc
    27601 catcccggcc cacccggttt accccgatca catacgattg gttttcgata gcacgcgcaa
    27661 tgagcaattg cctgaaagca tagcttcgtt tgccgggcca gttggcaacg tagatcaaca
    27721 ggtcatattc ataggaattg ttgaaatacc tgtttttgct ccataccggg aaacgcaggt
    27781 cgtaacagat cagagggcgg attttccatc ctttcagttc cacaaccatc cgttggtcgc
    27841 cgggggaaaa ttgcaggtgc tcgtcaccaa aacggaaaag atgacgctta tcataatatt
    27901 ccgaactgcc gtcgggtcgc atccagatca accggttata ataatcatcg cccgatttca
    27961 cgatacaact cccggtaatg gcgcatccat ggtcggccga tattttttgc atccaccgga
    28021 aagtctggcc ttccgcctct tcggcaactg aatgcggctc aaccacaaaa ccggtgctga
    28081 atgcttccgg caggatcacc aggtcgggtt tggttttcag tttcgagata atcccttcga
    28141 atttcgcaag gttcccgggc ttgtcttccc aggcaaggtc aggttgtata agggtaattg
    28201 aaaggttatc catagaacga aatatttgac aggcccaatg ccggggaatc cggcggaaat
    28261 gccgaaaacc gggtttgagt cctgaatttg gtttcccggc acaaaagtaa gaaaaatggc
    28321 gccttgcggg gtgctaaggc tcctgcgagt ccacgtccaa tatgacatga ccctcctgca
    28381 agccttcatg tgtcagaaaa ggtgccctgg taaacagtcg ttacactttt tacatttgca
    28441 cgtctgatat cctgtgactg aatgctttat cccggcattt taactactgt ctaggaatca
    28501 gggccctaat ttcataattt tgtggccgga aatattcccg gaaaattaaa ttcattcatc
    28561 atgaagacca ttgacgatta tacctttacg gggaaacgcg ccatcgtacg tgtagatttt
    28621 aacgttcccc tcgacgaaaa cttcaatata accgatacca cccgcatcga cgctgccatt
    28681 ccttccatca gtaaaatact ggcttcgggc ggcgccgtta tcctgatgtc gcacctggga
    28741 aggcccaaag gcccttcgaa aaagtattca ctcacgcata tccttcctta ccttagtaaa
    28801 aaactgggac gggatataaa atttgccgat gactgtatcg ggcagtcggc agccgatcag
    28861 gcagccgccc tgaaacccgg cgaagttctc ctgctcgaaa acctccgttt ttatgaagaa
    28921 gaagaaggca agcccaacct tcccgatact gctaccgacg aagaaaagaa agccgcaaaa
    28981 gcgaaagtga aggaatcgca gaaggaattt actaagaagc ttgcttccct ggccgattgt
    29041 tacgtgaacg acgctttcgg tacggctcac cgcgcccatg cctccaccgc gctcattgcc
    29101 aaatatttcc caaacgacaa gatgttcggt tacctgatgc gcgacgaagt tgccagcatc
    29161 cggaaagtga tgcacgaagg tcagcggcct tttacagccg tacttggcgg ggccaaggtc
    29221 tcgagtaaaa tagagatcat caacaacctg ctcggcaaag tcgataacct catcatcggc
    29281 ggcggtatga tgtttacctt catcaaagcg caaggcggta aagtgggaaa ctcactggtt
    29341 gaaaccgact tactggaatt agctaaagaa atacttgccg gcgccaaaac caaaggcgta
    29401 aacctgattc ttcccgtcga tgccattgct gccagcgctt ttgccaacga tgccgaccgg
    29461 aaactggtaa aagccggcga aatccccgac ggctggatgg gcctggatat aggacccgaa
    29521 acggttaagc tgttcagcga agtgctcaat aactcaaaga ctattttgtg gaatggacca
    29581 atgggcgttt ttgaaatgga caacttccag gaaggcacca aagaactggc tatggccgta
    29641 gccaatgcca ccggcaaagg cgcttactcg ctcatcggcg gcggcgactc ggtggctgcc
    29701 atcaacaaat tccacctggc cgataaagtc agctatgttt cgaccggcgg tggcgccatg
    29761 ctcgaaagca tcgaaggaaa agaactcccc ggcgtaaaag ccatactcga agactaacaa
    29821 taacctgcaa tgaaaaacgg aggctgtctc aaattcaggc agcctctctt tttttgcctg
    29881 cactgtgaaa gttcatactt ttgaacctgt tcaacaatgc ctcaatgaaa aaccttttac
    29941 tactatcagt cctgatcagc ttcatgtcat tatcgggctt tgcccaatct gaatctgcga
    30001 ccattcaaac aagcactggt gttcaggatt actgcaatta tgtgaacccc tttatcggca
    30061 cccttcggat ggggcatacc tttcccggcg caaccgttcc tttcggcatg gtgcagctca
    30121 gccccgaaac caataatccg ccgatgtaca ttaacgggaa atacaatgcc gacgtttata
    30181 aatactgctc gggataccag tacgacgaca gtactatttt cggattcagc cacacccatt
    30241 tcagcggcac cggccactcc gacctcggcg atttcctggt gatgcccact accggcaagc
    30301 tgaacctcga acccggcgat gcaacaaaac cgcacagtgg gtatcattcg caatattccc
    30361 atgagaagga atcggctcaa cccggttatt acagcgtcca tctcgatgat tacaacatcg
    30421 gggcagagct taccgccacc gaacgtgtgg gatttcacca gtacactttc cctgaaaccg
    30481 acagcgccca catcatcctc gacctgatct caaatattta tccttacgat gaaaagaatg
    30541 tctggacatt cgtccgtgtg gaaaacgatt ccaccgtcac aggattccgg accaccagcg
    30601 gctgggctcg cacccggacg ctctattttg taatgaaatt ttcgaagcca ttcgtttcgt
    30661 atggtcataa aaaatacgat aaagttccgt ataccggctt ttaccgacgt ttcaacgaaa
    30721 atgaaaactt ccccgaaatg gccggacgga agattcgggc ctatttcaat tttaagacct
    30781 cagggaacga aaagatcagg gtgaaatttg ccctgtcggc agtaagtgtt gaaggggcca
    30841 tgaaaaacct cgaagccgaa attcctcatt gggatttcga aaaaacgaaa gccgaaagcc
    30901 gcgccaaatg gaaccgcgaa ctctcgaaag tgcaggttga aacccttacg cccgaagaca
    30961 gggtgacatt ttacaccgcc ctgtaccata ccatgctcag cccggtgatc ttcgaagatg
    31021 cggatggacg gtaccgcggc ctggatcagg aaattcacac ttctgacgga tttaccaact
    31081 acaccatttt ctcgctctgg gacacttacc gggccctcca cccgcttttt aacattattc
    31141 agccggcccg caatgccgat atgatcaact ccatgctggc gcattacgac cagagcgttc
    31201 accatatgct gcccatatgg agccattatg ccaatgaaaa ctggtgcatg atagggtacc
    31261 acgccgtttc ggtgcttgct gatgccatgg ccaaagacat cgccggcttc gacaggaaaa
    31321 aagcccttga agcggctgtc agcacttcaa cgcgcggcta tttcgacggc ctggattatt
    31381 acatgaacaa aggctatgtc cccgaagata aaagctcatc ctcggtatcg aaaaccctag
    31441 aattcgctta cgacgactgg tgtatcgcgc aaattgccaa agaaaacggc aacgaaaggt
    31501 actgccagga atatcggaaa cgttcgcaca atttcgaaaa ccattacagc ccggcatccg
    31561 tgtttctgcg tccgcggctg gccgacggtt cctggaagca gccatttgat gcgctggata
    31621 cgcacgacca gggttacatc gaaggcaatg cctggacata cagccttttc gttccgcacg
    31681 atatcgacag gctgattgaa atgaacggcg gcaacgcgaa attcatcgcc catcttgatt
    31741 cgctgtttac catgaaactc gacgacaaat acatagaaaa aaccgaagac atcacccggg
    31801 atggcattat tggtaactat gttcagggca atgaaccggg gcatcacatt ccctatctgt
    31861 ataattgggc cggtgcgcca tggaaaaccc aggaaagggt ccgtatgatc atgagtacca
    31921 tgtacggaaa caaggctgac gggctttgcg ggaacgacga tgccggacaa atgtcggcct
    31981 ggtatatctt cagcgccctg ggattttacc cggttatccc cggatcagac cagtatgccg
    32041 tcggcagccc gctgatcaaa aatgctgtta tcaatctgga agacggaaaa tcactggtca
    32101 ttaaaaccat gaaccagaaa gccggaaatg tgtatgttca gaaaatcctg ctgaatggga
    32161 aagaattaaa tcgcagaacc ctgacgcacg ctgaaataac aggtgggggc gaactggttt
    32221 attacatgtc ggccaagccg gacaggaaga ttttcataaa ataattccgg cattaaggca
    32281 cttttgacgt tttctcactt ccggcatcat cgtggttcat catgtccagc accttgccgg
    32341 aatatttttt cagcatggga taaatagccg gggaaatgga aaggaccggc ttgaaagata
    32401 tcgactcatt gcgggtttgc tggctcacca ggtgggcttt gggatcaatt ttaaccagta
    32461 ccagcagaaa agcgcttaca ataataagtc ctttcgctgc tccaattgct gcacctccca
    32521 gccggttcac cagggccaat cctgccgttc tggcaatttt ggataagaat tggcccagca
    32581 ggtaaaccaa cacgattacc agcacaaaag ttgttatata agctataaac ttaatattac
    32641 tgcttgtaag gttcagttta ttggtcagga aaacggccat aaattccgaa aaatttatgg
    32701 cagcataaaa gccgaacagc aggcctgcca gcatggctac gctgataaag aaacccctgg
    32761 caaatccttt ataggcaaaa aaggcaagag gagcaaggat aatgatgtcg aggatattca
    32821 tgctggtttt taatttgccg gattaattta cagagaacag caggctatga gaaagcgggc
    32881 gggggagaag aggaacggat ttactggcgg ggctttaatc tccgggtaag ttcggaggca
    32941 aacacaaagt tctgaagttc ggggctgtcg gtttgcagga tgttgttccg gtcgccttgc
    33001 caggtaatcc ggccatcgta cataaagtat attttttcgc ctgttttgag caccgagctg
    33061 atatcatggg taacaacaac agttgtgatg tccgattcat gtgttatctc gccgataagc
    33121 tcgtcgataa ggcaggccgt caccggatcg agccctgagt taggctcatc gcagaacaga
    33181 tacttcgggt tcagggcgat tgcccgcgcc agcgcaacac gctttttcat gccgccgctc
    33241 agttccgaag gcaacatctt attcacattc accaaattga cgcgtgaaag gcaccagttg
    33301 gcgcgttcca gtttttcttc atacgtcatg gcagtaaaca tggtgagggg aaacatcacg
    33361 ttttccacgg ccgtcattga atcgaaaagg gcgcctccct ggaaaagcat gccgatttcc
    33421 tgccgtatct gtttcatctc cgaataggac atctttgaaa acggccgccc gtcaaaacgt
    33481 acaactccgg tatccggttc aataagccct accaggcatt tgacaagcac cgtttttccc
    33541 atcccgctct ggccgatgat caggtttgtt ttccccttat cgaaggatac gttcacattt
    33601 tcgagtacca cccggtcggc aaacttcttg tttatgttga ttccctcaat catgaaagca
    33661 gcagttgggt gagcaccagg ttcatgcaga tcacggcgat gctgcttcgt accacgccgt
    33721 tggtactggc ttcgcccact tcgagggcgc cgccccgggt ataatacccg tgataggccg
    33781 aaatggatgc tatcagaaaa gcgaaaacaa tggttttgat gatggcatac cagatatcgt
    33841 acatgtgaaa gaacgactga atcccgtaca catactggta cgatggcaca agtcctgtca
    33901 gtatccctga aagccatccc ccgaaaacgg ccacgatcat actgagcgct atcaggatgg
    33961 gattgatgat catggaggcg atgatcttag gaagtatcag gtatgttgcc ggattgatgc
    34021 ccataatttc gagtgcatca atctgttcgg taacgcgcat ggtgccaagt tccgatgcaa
    34081 tacgcgaacc tactttcccg caaaggatca ggcttataat ggtaggggag aattccagca
    34141 ctaccgattg tctgacggaa aaacctatgg tatatagggg tataaaaggg gtatcaatgt
    34201 tgaaggcaag ctggatggta agcacggcgc ccatgaaaac cgaaataatg gcgacaatgc
    34261 ccaccgatgc cagccccagg tgctccatct ctataagaac ctggcggtaa tagatcgacc
    34321 gcttctcagg cttttgaaat gcctgctgca acatttgcat atatctgccc aaatgataga
    34381 aaaagttcat tgcgaacaat aattagcgct aaattagcgt aaatcttatg aagattgctt
    34441 tttgtccgat atatttgcgt ccggatactc atcatcaatt tgttaaaatg ctgaaaatca
    34501 acttttcaaa gaaacgtcga tgcctgttac cggccataat atttctgctg gtgatacttc
    34561 agtcatgcgc tgctaatcat tacaacagca ggagccgggg atacatgcgt actccgccga
    34621 gtacaggccg gaaccgctgc ggttgcctcc tccctgaaaa acatgttaat caaatagaat
    34681 acgcctgctt tcatgtacaa aaaggttgat ccgctgcagg caatcaggca gtatttccct
    34741 cccgaaactt tcaagagtgt agaagcgtgt ttcagaaatt atcgcttcga tttacgtatt
    34801 tcaagaccaa gaaaaaacaa actgggcagt tttcaccctg ttgccggaat caataatccg
    34861 ctgatcaccg tcaacgccga tcttgggaaa tacatgtttt tcctggtatt catgcacgaa
    34921 atcgcccacc tgatcattca tcagaaatac ggacgcagcg ttttgtcgca tggtgaggaa
    34981 tggaaagctg ctttcaggcg cctcgtcatt cccctgatgg ataaccggca actccctcct
    35041 gacctgacag cgcctctgca gcaatatttc atcacaaccc cggcaacttt tatccggcaa
    35101 acggaactcc tccgggtaat caaccggctg gatggcaaag gccatgtgga tacgctgact
    35161 gatatccccg tagatgccat ttttgccctt tcgaacggaa aacgtttcgt taagctggaa
    35221 atgaaacgca cacgttgccg ttgcctgtgc ctcgacaaca aacgatacta tgatgttgcc
    35281 tgcaacgccc aaatatttcc ggtaagcgat acccattagt gcggcaatgc ctatatttgc
    35341 ctcatgggtt ttaattcaat ggattacaac ttattattca gttaatacct gattatgaat
    35401 caaaacaact attgcatcat tatggccggt ggagtcggcg ctcgtttctg gcccctgagc
    35461 cgtaccaatc gccccaaaca attcatcgac attctgggga ccggtgaaac acttatacag
    35521 caaactttca ggcgattatc ggcttcctgt cctccggaaa atatttatat cgtaacccac
    35581 gaaaattacc gggaactggt cttgcagcaa cttccgaatg ccgatcctca aaaagtgctg
    35641 tatgaaccgg taaggcgcaa tacagctccc tgtattgcct atgcctgcca taaaattgct
    35701 gccgaaaata gtaaagccaa catcgtggtt gccccttcgg accacattat cctgcgggag
    35761 gatattttta ccgatgtgat taataccgcc cttgaagccg cctccgccaa cgactggctc
    35821 atcacattag gcattactcc aagccgtccc gataccggat acggatacat tcagtttaac
    35881 gacagcaaaa catacggccc aggcggcagg ctgaacaagg tgataacttt taccgaaaaa
    35941 ccgcaatacg acctggctgt ttctttcctt caaagtggtg atttcctttg gaattcagga
    36001 attttcatct ggtcggttca aagtattctt aaagcatttg agcagcatct tcccgaagta
    36061 gattccctgt tccgtgaagg gaaaaatgtt tacggcactc ctgccgaaaa agaatttgtc
    36121 aataaagcct ataccgtttg ccaaagcata tctattgact atggtgtaat ggaaaaagct
    36181 gacaatgtat atgtagtggc tgccgatttc ggctggagcg acctgggaac ctggggctcg
    36241 ttatttgatg cacgcataaa gaatgaagac ggcaactcca tcgaaggaag caacatactg
    36301 acgtgggaca cgaaaaacag catcattctc atgccggaaa ataaactggc cgtaatcgac
    36361 ggcttaaacg attatatcgt tgccgaggac aataacctgt tacttatctg ccgccgcggt
    36421 gacgagcagc gtatccgcga atttgttgac accatccgcc atgacaaagg cgagaaatat
    36481 gtttaatctg caaaaacgcc gcttcatcct gactataata acctgatatc catactaaac
    36541 gcctcatgaa aaagtactta ctactccttg cgattcttgt atcgctgcaa atgaaatcgg
    36601 ttgccgacga aggcatgtgg ttgcccctgc tcatcgaacg tctcaactat gttgatatgc
    36661 agaaggaagg gttgcatctt acggctgaag aaatttacag cgtgaaccat tcaagcctga
    36721 aagatgccat cattatgttt ggcggaggct gcaccggcga aatagtatct cccgaaggcc
    36781 ttattttcac gaatcatcac tgcggttacg gatccatcca gtcgcacagc accgttgatc
    36841 atgattatct caccgacggt ttctgggctt ttcgcaaaga agaagaattg cccaacccgg
    36901 gattgacggc acggtttctg atcagcatcc aggatgttac taccaccgtg ttgggtagcg
    36961 taaatgacaa tatgccggaa agcgaacgaa acgaaaagat taaaaaagct atccgggaaa
    37021 ttgaagaaaa cttttccaaa ggcacaaaat acaacgcccg tgtatcctct ttcttcaacg
    37081 gtaacgagta ctatctgttt gtgtatgagg tgtatcgcga tgtccgcctg gtaggggcgc
    37141 cgccttcttc catcggaaaa ttcggtgccg ataccgacaa ttggatgtgg ccgcgccata
    37201 ccggtgattt ttccattttc agggtttaca cggctaaaga cggttcgcct gccgactatt
    37261 ctccagataa tgtcccgatg aagcccaaac actacctgcc tgtttccctc aaaggcgtca
    37321 acaaaggaga ttattccatg atcatgggtt acccgggatc gaccgaccgg tttgccacct
    37381 cgtataccat cgactgggcc gttaataaac acaatcctgc cgttgtaaaa atccgcaccg
    37441 agaagctcgg tatcatgaaa aaggatatgg atgccgatcc ggcagtccgt atcaaatatg
    37501 cttcaaaata tgcaggaacg gcaaactact ggaaatttta tatcggacag aacaaaggtt
    37561 tgaaacgcct tgatgttgaa caacgcaagc gagaaaccga ggaacgcttc tcggagtgga
    37621 tcaatgccaa tcgcgaccgg aacctgctgt atcgcgatgc cctttacagc atttcaaaag
    37681 cctacgaaac ccttaaccgg tatgaactgg ccatgaccta taataatgaa gccatcaacc
    37741 gcggttgcga gattatttca ttcagccgtc agtttaattc actggcaata gagttgcaag
    37801 ccaaaaatcc ggaccaggaa gccattgaaa aaataatcgg gagatgccgg acatcgtcgg
    37861 atcagtactt taaaaactat aatgccggca cagataagca attgctggca gccatgctta
    37921 acctgtattt taccaacgtt ccccgcgatc agcaaccggc ttacctgaaa accttataca
    37981 gtaagaagac taacagcttc tcggattatg cagccagggt attcagcaaa tcaatctttg
    38041 ccgaccagca aaaactcgaa gcttttctgc aaaatcccaa cctgaaaaaa ttgcagaaag
    38101 acccgatatg gcctttacaa atagcttttg ccgaaaacgc cggtaaaatc gactccctaa
    38161 ccaggccggc cgatgaactg ttatccagag gcaaacggtt gtttgtgaga ggactccggc
    38221 aaatggaagc cgataaaagt ttttatcccg atgccaacag caccatgcgg ctcacttatg
    38281 ggaaagttga cgactacagt ccccaggatg ccgttgatta tgattttgtc acaaccctcg
    38341 acgggatcat gcagaaagaa gatccctcaa gctgggaatt tgtggttcct gcaaaactca
    38401 agcagttata tcaggataaa gattacggca ggtatggtaa aaacgggaaa atggtggttg
    38461 gtttcatcac caataatgat atcacgggcg gaaattcagg aagcccggta ttaaacgccg
    38521 atggtgaact gattggactt gcttttgacg gcaactggga ggctatgagc ggcaactatg
    38581 ctttcgaacc tgacctccag cggaccattt gcgtcgatat ccgatatgtg ctgtttatca
    38641 ttgataaatt tgccggggca aagaacctga ttgatgagtt gacgctgaaa gaataaatga
    38701 agtaaaacct gccttttcct gtcaagactg cctgcactta ctctcaacac tgcctcatgc
    38761 cgggtatcgg ggtactgttg ccggctgata gctgtatatt gcgtaagcca tgacagccgg
    38821 gggcttttat cgtaatccgt caaatatcaa aatgtggcca tagaaaccga acgcaagtac
    38881 ctggtccgca cctgtttgct gccgcccctc ggagacggga agcagattgt gcaggcttac
    38941 ctgcatgtaa gtaaacaggt aactatacgg gcacggattg agggtgaatc ggcatacata
    39001 actgttaaag gatttcagga taagttatca agacctgaat ttgagtaccc cgttcccatt
    39061 gacgatgcta ttttcatgat caatagcatg agtccatacc ccatcatcag gaaaatcagg
    39121 cgggaaatct tctgtcacgg aaagtgctgg caactggatg aattccagga gatgaacgcc
    39181 gggttgtgga ttgccgagat agaacttact gatcctgacg aaccggtcga attgcctccc
    39241 tgggtttccg acgaagtgac gggcgatccg cggtacctga attccgggct ttcaataaac
    39301 ccgtattgca actggaacaa cggcgaaaaa atggataatc tgctcaatac gtgaaaaatt
    39361 aaatagtctt tatagctaaa aatacaaata tcaaaacagg ttactcttat ttttgtatca
    39421 cataaacgga at
//
LOCUS       DAOPYV010000027        36594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_1041_length_36594_cov_11.931470, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000027 DAOPYV010000000
VERSION     DAOPYV010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 36594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36594
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1041_length_36594_cov_11.931470"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="PKI35_05190"
     CDS             <1..1351
                     /locus_tag="PKI35_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNX43814.1"
                     /translation="SFPYICLIKSPSVLLVLAPKITNRLRYIFNLLLNDLLGLETCFT
                     ASLDEFSTYEGAKFSYGVSLESDALFFAANHLLFETGISSCEVSCFDYAGSKAFFAVY
                     QKSSAMPFDVFAASFFLVSRYEEYLPQMRDEHGRFRAMQSEAYRNGFLRKPLVNSWAL
                     ELKKVLQDRFPELEFSLPSFKFIPTIDVDAAFAYKHKGMTRAVGGFFKAINRSDYQEL
                     RERIEVLLGRRADPFDTYEVIMALQKKYSLKTLFFILLADYGVNDKNLPVQNRYFRQI
                     IRELADYADVGIHPSYASSGNAAVLQKEISRLSGILHRDILKSRQHFLRLEMPTTYRN
                     LINCDIAEDYTMGYAEEAGFRASICSPYNFFDLDLDRKTPLRIFPFALMDGTLNDYMD
                     LTPVQASDLISNLMKEVKAVGGTFISLWHNSTLNDQRHWKGWLMVYEDMIRLAVKGK"
     gene            1365..2306
                     /locus_tag="PKI35_05195"
     CDS             1365..2306
                     /locus_tag="PKI35_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43815.1"
                     /translation="MIKYIEHGEIRKNRWDDCIAGAFNGCVYAYSWYLDIVCPAWGAL
                     VEDDYLRVMPLPFFRKCATVYSMQPVFTQQLGVFSKDELTPDLIENFIKAIPDEFLLI
                     DYNLNIHNQLQPGNYKLFPMADYELNMADDYEKLKSGYSQNTRRNLRKGAENRLVLST
                     GIKPDDVVKLFRNGSGKEYKNLGDKQYATLLRLVYQCLSRGMAVVWGVYDRHLELCAG
                     AIWVFSHQKAIFLFSGLSDKGRLSGAMPFLIDNFIREHAGSRLTLDFEGSNNEGLARF
                     YAGFGSSRLIYPRLVINRLPLAVKIALPVVRRIRNIF"
     gene            2403..3059
                     /gene="upp"
                     /locus_tag="PKI35_05200"
     CDS             2403..3059
                     /gene="upp"
                     /locus_tag="PKI35_05200"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNX43816.1"
                     /translation="MVINLGNQQSFVSQLVSELRDAKIQSDSMRFRRNLERIGEFFAY
                     EISKKLHYENREVVTALGTATVPVLKEFPILATILRAGLPVHKGLLNIFDKSENAFVS
                     AYHDHSKEDQFMVDVEYVSSPELTGRTLIISDAMLATGHSMFQTYKALIGIGKPAHTH
                     IVSVVASAQGIEYLKSSLPKTGTTIWVGAIDDELTAHGYIVPGLGDAGDLAYGKRHDF
                     "
     gene            3150..3647
                     /locus_tag="PKI35_05205"
     CDS             3150..3647
                     /locus_tag="PKI35_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43817.1"
                     /translation="MTSFVKFLLILATSSIKFLFAPPLSYGFGFGFLQTWAFTSVGGV
                     LGVLFFFMLSKILINIYVKYFRTSFRERLLQLAGRIGQRHIAERYLPKQTRVFTRQNK
                     IIAWIRSRLGFTGVIILAPVLFSVPLGTLIVMRLYAGKKYLPVYLSASVVAWSLVIST
                     SIALF"
     gene            3839..5095
                     /locus_tag="PKI35_05210"
     CDS             3839..5095
                     /locus_tag="PKI35_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX43818.1"
                     /translation="MASSFFSGLVYENVRISLGSIRSHMLRTILTVLIIAFGIMALVG
                     ILTSIDAIRYYLNENFQMMGANTLTIRNRTLQVHMGGERSRPKFFREITFDEAMRFKQ
                     EYSFPANVSVFIRATGTATIKYLSNKTNPNMPVFGTDDSYLATSGYEMDKGRNFTPSE
                     IYYGSNTAIIGTDVVKRIFKNKEDPVGKFISIGPGRYLVIGVLKEKGSSLGSNPDAIC
                     LLPINNVRQKFSRARASYSINITTNDNSLLDAAEGEAAGLLRIIRKQSVGEEDSFDIS
                     KSDNLANMLFENLKYLRLAATIIGIITLIGAAIGLMNIMMVSVTERTREIGIRKAIGA
                     KRHTIRNQFLVEAIVIAQIGGALGIIFGIAIGNSLSMAIGSSFIIPWSWIGLGVTLCF
                     LVALVSGILPATKAANLDPVDSLRYE"
     gene            complement(5103..6476)
                     /locus_tag="PKI35_05215"
     CDS             complement(5103..6476)
                     /locus_tag="PKI35_05215"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HNX43819.1"
                     /translation="MNRFSITIFFLALTSIIQASCLMAGPLYPAPASSGSQEVKPALP
                     DTTDYYSPEFIRNEDVVYLKNIRSVVFRQSDVELSAPVITLNSGETLHLEFDDLNGNI
                     EPYLYTVIHCDANWKTSDLWPNEYIEGLQEDNIEDYQFSFNTLQHYTHYQFGFPNEKM
                     KILLSGNYILKIFKREEKDKETVVLTRRFMVADPKFIIDAKITRASTIEDIETRQEID
                     FTVNSAGYRVESPYQDIHVTILQNNRWDNSLTTLKPFMVRGDVLDYSFDNGINQFDGG
                     NEFRFIDLKSVKMVSGNIRSINMNDTTYLVTLWDNERRTFKVYVNNSDIDGRFLLKTD
                     DESSVETMGEYASVRMFLRYDAPVVHGQLYVAGAFNGWQYNDENLMHYNYSRHGYEAT
                     LMLKQGYYDYAYMFLPNQSATGDITYIEGNHSQTQNTYTILVYQRERGTTYDQLVGMG
                     NYTSRGK"
     gene            6680..8020
                     /locus_tag="PKI35_05220"
     CDS             6680..8020
                     /locus_tag="PKI35_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HNX43820.1"
                     /translation="MDGFIKIKKGLDIKLLGEAQNKVTEILTDDFALKPTDFIGVFPR
                     LLVKEGDIVKAGSPVFCDKYRESVQFTSPVSGKISEIRRGDKRVLLEVGIQADKDIVF
                     EDFGREDMASLGRRQLIDKMLLSGVWPAIRQRPYTVIANPDDTPAAIFISGFDSAPLS
                     PDYAFILKDELAGFQAGVDVLARLTSGKVYIGIPEFNHPLAGIRNAVITCFKGPHPAG
                     NVGIQIHHIRPINKGDIVWTVNPQDVVVIGRLFSSGRYDASVTVALAGSEVREPQYYK
                     VRKGISVKPLISGNLKGDTQRIISGNVLTGDRISHNGYLGFYHNQFTVIPEGNYFEFM
                     GWAMPGFGKFSFSKTFFSWAHKDKKYKLDTNFHGGERSYVMTGQYEKVLPMDILPMQL
                     IKACLLEDVELMEKLGIYEVDEEDFALVEYIDTSKTEIQSIIRKGIDLIRKEMS"
     gene            8138..9304
                     /locus_tag="PKI35_05225"
     CDS             8138..9304
                     /locus_tag="PKI35_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HNX43821.1"
                     /translation="MKALRNYLDKIKPRFQEGGKLEKFHSTFDAFESFLFVPDKTTFK
                     GSHIRDSIDMKRTMTVVILALMPALLFGMWNVGFQHFRATGQEGGFWQFFGYGLLKVL
                     PIIVVSYVTGLIIEFAFAQYRKHEVNEGFLVTGMLIPLVMPPDIPLWMVAVSTAFAVI
                     LGKEAFGGTGMNVFNPALLARAFLFFAYPAFMTGDNTWIAGLASGTGIVDGYSGATPL
                     ANAATEAIDKIPPLSDLFFGFIPGAIGETSKLAVLLGAALLLITGIGSWRIMLSALTG
                     GVVMTLIFNAFAANKFMAIDPLTQIMMGSFLFGVVFMATDPVTAAQTNKGKWIYGFLV
                     GTFAIMVRVFNPAYPEGTMLAILLMNTFAPLIDHYVIEANIKRRLKRAKAVVKS"
     gene            9319..10038
                     /gene="nqrC"
                     /locus_tag="PKI35_05230"
     CDS             9319..10038
                     /gene="nqrC"
                     /locus_tag="PKI35_05230"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HNX43822.1"
                     /translation="MEHSNKYIFVYSLVMVVLVAVSLTIVAVQLKPAQDDNIRIEKMQ
                     NILSSVNVPVAEIPKKQVIDVYNKYVKTAFAVNVNGDVTETDAEKVFKLEMANELKKE
                     PSQRQLPVFVAVLDNGDSAYIVPLRGKGLWGAIWGYLSFKTDFNTVTGCMFDHKSETP
                     GLGAEINQPWFQKPFAGKKIFDDQGRLVSVGVVKKGHPKDDTHDVDAISGGTITSKGV
                     QKMIKDNLDGYQAYFKKHLKQ"
     gene            10035..10697
                     /locus_tag="PKI35_05235"
     CDS             10035..10697
                     /locus_tag="PKI35_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HNX43823.1"
                     /translation="MNKEKLRKEKDPWFSAKNLSLLKRPLNIENPVTVQVLGICSSLA
                     VTVQMKPAIVMALSVTIVTAFSNLIIALIRDSIPSRIRIIVQLVVVAALVIIVDQILK
                     AYVYDVSKKLSVFVGLIITNCILMGRLEAFAMANKPWPSFVDGIGNGLGYGLVLVTVA
                     FFRELLGSGKFYGMKVIPEAFYKAGYVDNGLMILPPMALITIGIIIWIQRWKKPELQE
                     KN"
     gene            10734..11351
                     /gene="nqrE"
                     /locus_tag="PKI35_05240"
     CDS             10734..11351
                     /gene="nqrE"
                     /locus_tag="PKI35_05240"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HNX43824.1"
                     /translation="MDNLFNLFVRSIFIDNMIFAYFLGMCSYLAVSKTVKTSLGLGVA
                     VTFVLLITAPVNWLIDTYMLKAGALAWLSPSLADLDLSYLSFLIFIAVIAAMVQLVEM
                     VVEKFSPSLYNALGIFLPLIAVNCAILGVALFVQEREYTSIGEVTVYAVGSGLGWLLA
                     IVSLAAIREKIRYSAIPKPLRGLGIAFILTGLMAIAFMSFMGIKI"
     gene            11396..12667
                     /gene="nqrF"
                     /locus_tag="PKI35_05245"
     CDS             11396..12667
                     /gene="nqrF"
                     /locus_tag="PKI35_05245"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HNX43825.1"
                     /translation="MITSVILLLGKLAGPLLIGTSVFLFVTLLLVTMLLVAKAKLAPS
                     GPVKITINGEKELTVNAGSSLLTTLANEKLFLPSACGGGGTCGMCKCQVHEGGGSILP
                     TEVGFFSRKQQLSDWRLGCQVKVREPLNITIPKEVFGIKKWECEVISNHNVATFIKEF
                     VVRLPEGETLDFQSGGYIQIDVPKMEVDYKDIQVEPEYQEDWNKLKMWDLKMKNPEPV
                     FRAYSMANHPAEGNIVKLNIRIATPPWDRAKNQFMNVNPGICSSYIFSRKPGDKVTVS
                     GPYGEFHIKPTRKEMVFIGGGAGMAPLRSMIFHLFFTEHTDRKVSFWYGARSLRETFY
                     DDEFRDIQEKNPNFSFNLALSEPLPEDNWKGYTGFIHQVVLDKYLSNHPEPEEIEFYL
                     CGPPMMNAAIFKMLDNLGVPPENIAFDDFGG"
     gene            12801..13814
                     /locus_tag="PKI35_05250"
     CDS             12801..13814
                     /locus_tag="PKI35_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNX43826.1"
                     /translation="MKKLSALVVGAILAACVLSGCKAKPKLVKFVGETQGTYYAITYY
                     AKDGVNYQLQIDSLLHRFDSSASMWKPNSLLSRINRNDTTVRADETLKTLFRKSMEVS
                     EQSDGAFDITVGPLVEAWGFGFTSRMKVDKHVVDSLLPLVNYKKLKLVNEKIAKSDPR
                     MQMDFNAIAQGYAVDLVGKFLESKNIESYLVDIGGEVLGKGTKPEGGEWNVGIELPAA
                     DSLAERQVKAIVSLRDKALSTSGNYRKYYEENGVRYSHTIDPATGYPVKHTLLSVSVL
                     ASDCMTADAYATVMMVKGLEDSKPFLQAHPDVEAYFIYSDTTGQMKTWFSPGFKKLIE
                     EEL"
     gene            complement(13880..14062)
                     /locus_tag="PKI35_05255"
     CDS             complement(13880..14062)
                     /locus_tag="PKI35_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43827.1"
                     /translation="MLKLVLAAIVLVGLAFAGIAIRMFFIRGGEFRKSCSSRNPETGE
                     RMDCSCGGDSARCNNS"
     gene            complement(14088..15581)
                     /locus_tag="PKI35_05260"
     CDS             complement(14088..15581)
                     /locus_tag="PKI35_05260"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNX43828.1"
                     /translation="MAPLSNLINYHIEELKKINNLSERTKNVCLEGSLDTLYKILYYY
                     LERGTFRDIRNCGLKTDLELIGLSQKYLEISGLTLESLRMNEPLLGFEDFKLFCYVNF
                     KVPSEVTEVYKEQFINKEFQFFKYLKTLISYVLNEREQYIFMNNFGFFNNSQKRTLQS
                     IGDQYGITRERVRQIAQKIPEKLENIVSKISQRFTTIEKHFDYGLDLKKELLVIDNDL
                     VQKINKLEGVSFTGKFYCLVFAGTFQRYYMSFQDLEENYNYYYLVSRKYKEIFDFKKY
                     FADIEATKETRIEKSYQVDAQEYINKFMYPHQTGSDRLKQICRRMATEEFGLGFDRST
                     ITFNRNTLIKLSEHILKILEDVGHPMKLTDICKELKNRTTRIPPNIESLRSSILSIDE
                     VVAIGKTSTYALKKWQAVKTGTIKELVKELLEASDEPKHISDITVYVCKFRNTSDKNI
                     LSNLKLDKTNTFVFFRKSYIGLKYKQYTNIPTGSDKAGRGRKPKISQ"
     gene            complement(15662..16444)
                     /locus_tag="PKI35_05265"
     CDS             complement(15662..16444)
                     /locus_tag="PKI35_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNX43829.1"
                     /translation="MLSKNQIKYINALKIKKFRQQYGIFLVEGEKCVDELLHSSLEIT
                     GVYALPDWLQEHSRLLKARSISFTEIDETILEKISDLTTPNKVLALAKVPEPAMLPDV
                     YNSYCLALDNIKDPGNMGTIIRTADWFGIRRIICSPGCVDVFNPKVIQSTMGSFTRVS
                     VFQMDLIQFIKQRPKDTPVYGALLQGRNLHQVAFNEAGIVVIGSESHGISAGLLPLVT
                     QAIYIPPFNINGAISAAESLNASIANAIICYEISKQLSEKKS"
     gene            complement(16507..16956)
                     /gene="rplI"
                     /locus_tag="PKI35_05270"
     CDS             complement(16507..16956)
                     /gene="rplI"
                     /locus_tag="PKI35_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNX43830.1"
                     /translation="MEIILMKDVPTLGYAHDVITVKDGYGRNYLIPQGFAILATESSK
                     KMNTEIIRQKSFKDQKANADASTLAKKLENITVKIGAKAATTGKIYGSVNAIQIADAI
                     KEQFNYDIDRKKIYVDGESIKELGTYKAAIKLHKDIQVSINFEVVAE"
     gene            complement(16968..17240)
                     /gene="rpsR"
                     /locus_tag="PKI35_05275"
     CDS             complement(16968..17240)
                     /gene="rpsR"
                     /locus_tag="PKI35_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNX43831.1"
                     /translation="MANQGNSEIKYLTPITVDVKKKKYCRFKKSGIKYIDYKDADFLL
                     KFVNEQGKILPRRITGTSTKYQRKVAQAIKRARHLAILPYVADLLK"
     gene            complement(17267..17629)
                     /gene="rpsF"
                     /locus_tag="PKI35_05280"
     CDS             complement(17267..17629)
                     /gene="rpsF"
                     /locus_tag="PKI35_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNX43832.1"
                     /translation="MVNQYEAVFIMTPVLSEEQMKETVSKFQKFLKDKGAEIVHENNW
                     GLRKLAYPIQKKSTGFYHLIEFKAEGSTIADLETTFKRDERILRFLTVALDKHAIAYN
                     EKKRANKPAEAAEAAPQA"
     gene            complement(17968..19113)
                     /gene="wecB"
                     /locus_tag="PKI35_05285"
     CDS             complement(17968..19113)
                     /gene="wecB"
                     /locus_tag="PKI35_05285"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNX43833.1"
                     /translation="MKIITIVGARPQIIKAAALSRAIREQFNAEIEEIIVHTGQHYDK
                     NMSDVFFQELDIPRPHHNLNIGSASQGNQTARMLLGIEDILMQEKPDFLVLFGDTNST
                     IAGALAGVKLHVPVVHIEAGLRSFNKNMPEEINRIVCDQSSTLLFVPTRAGISNLQKE
                     GFHTNNEAPFSLNHPGIFHCGDIMFDNLVYFAGIAEERTTILRELSLKDKPFVLCTIH
                     RDNNTDNPQRLNAIFSAIATIASTRNLEIVIPLHPRTARLLKSQPGPDLYHRLINDRR
                     IRLIPPVSYLEMVALEKNASLILTDSGGVQKEAYFFKKPLVVARPETEWVEIIKNGAG
                     ILADADERRILESADYFFGEAAPNFPDVFGDGHAAEFICRTMLESIS"
     gene            complement(19183..19878)
                     /gene="radC"
                     /locus_tag="PKI35_05290"
     CDS             complement(19183..19878)
                     /gene="radC"
                     /locus_tag="PKI35_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNX43834.1"
                     /translation="MSETGRTPIKLWLEDDRPREKLLIKGPAALSDAELIAILINIGT
                     RELSALDLAQEILNKAEGNLIELSRLSVKDLLKFKGIGEAKAITIAAALELGKRRRSS
                     EILDRRTIRSSHDAFELLQEKLADLNEERFCIILLNRANRVLNVEMVSTGGFAGTVAD
                     PKKIFKIALDAKASSIILGHNHPSGMTKPSEADNKLTSKLKSSGDILDLPVLDHIIVG
                     DEKYFSYADEGMM"
     gene            complement(20014..20265)
                     /gene="rpsT"
                     /locus_tag="PKI35_05295"
     CDS             complement(20014..20265)
                     /gene="rpsT"
                     /locus_tag="PKI35_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNX43835.1"
                     /translation="MANHKSSIKRIRANEKKRLRNRYYAKTMRNAVKSFRLLTGKSEA
                     AEKLPTIVSLIDKLAKKQIIHKNKAANLKSKLAHKVNTL"
     gene            complement(20288..20359)
                     /locus_tag="PKI35_05300"
     tRNA            complement(20288..20359)
                     /locus_tag="PKI35_05300"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20324..20326),aa:Glu,seq:ttc)
     gene            complement(20613..21668)
                     /gene="waaF"
                     /locus_tag="PKI35_05305"
     CDS             complement(20613..21668)
                     /gene="waaF"
                     /locus_tag="PKI35_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNX43836.1"
                     /translation="MNPPRKILIARLSSIGDIILTTPLVRCVRQAYPDASITFLVKKK
                     FSSLVSFNPNISEVIEFDDVKGRNELRVLARKLRREQFDWFIDIHRSLRTKYIRLNVR
                     FGEVTSYHKRILLRTLLIKLGINRFKSVKPVFLRYFEAVESHGINYDNNGTEVFYSDD
                     DRQTVERFLIPEGFIPQQKMVVICPGASYSNKQWLPERFAQLADRLVQNNGVFIVFLG
                     GPSDTGLCGHIITMMKNRATNFAGRLTLLQSAALLNKSSLVITNDSGMMHLAQSQKRP
                     VAAIFGATSRELGFFPLPEKSIVVEKEMPCRPCTHKGLDHCPRKHFNCMRLITTNDVI
                     DAVEQMLAELSGSVIKG"
     gene            complement(21717..23045)
                     /gene="xylA"
                     /locus_tag="PKI35_05310"
     CDS             complement(21717..23045)
                     /gene="xylA"
                     /locus_tag="PKI35_05310"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNX43837.1"
                     /translation="MNYYTGTSEFFPGIGKIPFEGKGSKNPMAFKYFDPNQMVAGKPM
                     KDHLRFSMAYWHTLCGDGGDPFGKATFNFPWNQSRDAMEKAHNRMDAAFEFMTKIGLN
                     YYCFHDFDLVEEGSTIAESEKRLHEILEYAKKLQAQTGIRLLWGTANLFSHPRYMNGA
                     ATNPDLQVVAWAATQVKNAIDATIALGGTNYVFWGGREGYITLLNTDMKRELEHMGRF
                     LTMARDYGRKNGFTGTFLIEPKPMEPTKHQYDFDAATVVGFLREYGLEKDFKLNIEVN
                     HATLAGHTFQHDLQVAANAGMLGSIDANRGDYQNGWDTDQFPINIYELVEAMLVILEN
                     GGIQNGGINFDAKRRRNSTDPEDLFIAHIAGMDAFARALLIADRILTASPYLAMKKQR
                     YASFDKENGKAFEQGRLNLEDMRKLAAENGEPATTSGKQELLESLINDYI"
     gene            complement(23058..24545)
                     /locus_tag="PKI35_05315"
     CDS             complement(23058..24545)
                     /locus_tag="PKI35_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNX43838.1"
                     /translation="MFFLGIDIGSSSVKTCIINGDTGNVTASAAYPPAEMEILAPSPG
                     FAEQHPDEWYRNLAEALKAIGSENPALLQQVKAIGIAYQMHGMVAVDKQGKTVRNAII
                     WCDSRAVKIGEKAFGELGHEFCLSHLLNSPGNFTASKLRWVKENEPDVYDRIHKIMLP
                     GDYIAYRLTGEICTTQTGLSEGIFWDFPKNSISNELLDYYGIDPNLLAEVKPVFAAQG
                     VLTAEAAGNLGLKPGIPVTYRAGDQPNNAFSLNVLNPGEVAATAGTSGVVYGVTDRMK
                     YDPKSRINTFLHVNHSKQAPRLGQLLCINATGILNAWIRNTLMPAGMSYGEMNKKAAL
                     VQPGADGLRIIPFGNGAERMLENKNPGAVIEGLNYTIHNREHLLRAAQEGIVFSFIYG
                     MEIMKQSGLNLSKIKAGKANMFLSPLFCTMLSGLSGAEIYLYNTDGAQGAARGAALGA
                     GFYRNAGEAFNSLKVLDEIQPEKDNKLSDTYLHWKNRLEQKLKEL"
     gene            complement(24692..25387)
                     /locus_tag="PKI35_05320"
     CDS             complement(24692..25387)
                     /locus_tag="PKI35_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43839.1"
                     /translation="MMDILPNEDLLGGLAKKFSFKQQVYRNTAETFNCFKSIMKKMSD
                     EFRTKYNTMEPEVPFEFNDISEFEAELKFGSDVLIFMMHSNVFEFSRDHEVMKTPYVR
                     QDPNRSYCGMIMIFNFLADSFRYNRLNDLGYLIGRVFINNENHYFIEGKREIGLLYNS
                     FQSAVMNPESARQIVDSAVRYTVNFDLLTPPYDNVKVVSVGDMMSAIDSTKIRTAKRL
                     GFRFQGDHEEFGS"
     gene            complement(25451..27040)
                     /locus_tag="PKI35_05325"
     CDS             complement(25451..27040)
                     /locus_tag="PKI35_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNX43840.1"
                     /translation="MRKEIEIRMTPRQAADKASQKDFFAQQLRIPVSRIKFIRPIQRS
                     VDARSKNIKISLKAEVFIDELPVGALKPVKKNYPDVSRKQPVIIIGAGPAGLFAALTL
                     IGNGLKPVIFERGKDVKSRKSDIARLNREGLINPDSNYCFGEGGAGTFSDGKLFTRSS
                     KRGNVSEILETLVVHGANEDILIDSHPHIGTDKLPAIIQAIRNTITESGGEFHFNCRI
                     TDIIIEKGKACGVTDLNGDRHDGAAVIIATGHSARDIFELLHNKGLQLEPKSFALGVR
                     VEHPQPLIDSIQYHSSPGTLRNSFLPAASYSLVKQVEGRGVFSFCMCPGGTIVPAATE
                     SGQVVVNGMSNSRRNSPYANSGIVVSIEQEDIQHFSKHGPFAGLEFQRQVEEACWQAA
                     GRTQKAPAQRLDDFVDGVFSSTLPECSYHPGVIPAALPELLPFSVTKRLREGFRLFDR
                     QMKGFITREAIILGTESRTSSPVRIPRNEHTLEHIQVADLYPCGEGAGYAGGIISSAI
                     DGVRCAHAIAQKQSGLRLSPL"
     gene            complement(27125..27907)
                     /locus_tag="PKI35_05330"
     CDS             complement(27125..27907)
                     /locus_tag="PKI35_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNX43841.1"
                     /translation="MKNYRTILPLITILALCSIGDLSAQTKVPNLNNFDQSPYHFGFQ
                     LGINKMFFTVETKQNFQEVTYVNNSNIDQIPDLTCDTAHLFGVLANPTYGFLIGIVSN
                     LRLGEYFDLRFTPSLTFGERGLTYSIQNSEGSSEPTLDEVTKNIQSTFVDFPIWVRFK
                     GERIHNVRPYVLAGAKYSLDLASNAKKKDNSTATHVFLDKHDVYGEIGVGFDFYNAVF
                     KFGTELRMSYGLFDVLKHDKTIYTGGIENLNSKIFQLVFTFE"
     gene            complement(28000..28740)
                     /gene="ubiE"
                     /locus_tag="PKI35_05335"
     CDS             complement(28000..28740)
                     /gene="ubiE"
                     /locus_tag="PKI35_05335"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNX43842.1"
                     /translation="MSRRINELTVIQAAGKKEQVETMFNDIAPRYDFLNHFLSAGIDR
                     LWRQTLVKTLAQYPHRNILDLATGTADLAIAASVLNPENITGIDIAGEMLNIGNEKVK
                     RKGLGNIIKLLPGDSEKLPFADCAFDATMVAFGVRNYENLNQGLSEMHRVLKPGGGTY
                     ILEFSMPTQFPVKQVYHFYFNYILPALGSFVSKHRSAYTYLPASVIVFPQGDRFLSIM
                     KDIGFINVSARKLTFGIASLYTGFRKNG"
     gene            complement(28781..29791)
                     /locus_tag="PKI35_05340"
     CDS             complement(28781..29791)
                     /locus_tag="PKI35_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNX43843.1"
                     /translation="MFKNREEISDLFERFANLNVLVVGDVMVDAYLFGKVDRISPEAP
                     VPVVALQKRESMLGGAANVALNIKSLGANPILCSVIGNDPHGKELIDLLTGNDLDTGG
                     IIQSSQRITTTKFRVIGNRFQMIRVDEEIDSDLTKSDSDALLLRFEELLYQAKPAVIV
                     LQDYNKGVLTHGVIDTIMIKALQAGIPVVVDPKKKNFTAYRSATLFKPNLKEIREALN
                     TDIDPYDKSSLSNAAKKLHNLLDAKHIMITLSENGVFMSSRFNGTEDTFILPSHVRNV
                     SDVSGAGDTVISVASLCMALGIAPDKTAWISNLAGGLVCEEVGVVPVNRQKLIREAQS
                     LI"
     gene            complement(29824..31032)
                     /locus_tag="PKI35_05345"
     CDS             complement(29824..31032)
                     /locus_tag="PKI35_05345"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNX43844.1"
                     /translation="MENNFLSDRINALAESETLAMTRRSRELKAQGFDVINLSIGEPD
                     FNTPEHVKDAAKEAIDQNFSHYPPVPGYPELREAIALKLKRDNNLDYKADQIVVSTGA
                     KQALANAILCLLNPGDEVIVPAPFWVSYKELIKLAEAKAVYINTTIASDYKVTPSQIE
                     AAITPSTKLFIFNSPCNPSGTVYTHEELKAFAEVFERHPNIFIISDEIYEYINFTGKH
                     YSIASFGSIHDRVIIINGLSKGFAMTGWRLGYLAACREIAKACDKLQGQFTSGTCSIS
                     QRAAITAMKTDPAESEDIKSMIHAFKYRRDLAISMLKQIPGITVNVPDGAFYLFPEIS
                     QYFGKSNGSHVINSGADLCDYILDTAYVALVPGSAFGTPNCIRISYATSEDNLQEAIR
                     RIETALSKLS"
     gene            31245..31769
                     /locus_tag="PKI35_05350"
     CDS             31245..31769
                     /locus_tag="PKI35_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43845.1"
                     /translation="MNLILQLSQIFLPVIAVLAVTYYLVKRFLDHHTEQQEMNMKMKL
                     HESVTPIRLQAYERLMLLLERMQPAGMLMRHNRSGMNAIELQTALLQGIRDEFEHNLS
                     QQLYVSIQAWEKVRNAREEMISLINTAAAKVKPDAPSADLAQNVLALSLEVEILPVQQ
                     AIDFLKIEVRQNFF"
     gene            31818..32735
                     /locus_tag="PKI35_05355"
     CDS             31818..32735
                     /locus_tag="PKI35_05355"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNX43846.1"
                     /translation="MITYNPATLTPIQMLRLLQGTVMPRPVALVSTLNRQGYPALFPC
                     SYYTIAGSDPGTLVFSPVRNRKDPSAKDTILNIKEIPEVVINAVSYRMVEQANLAGHE
                     FARATDAFLKAGFTALPSEKVRPFRIQESPVHFECAVKQVVETGKPGAADMLIICEVL
                     LIHIDDSAIDSQGRTNPDKLDVVGRMGGDVYVKASGKALFRVRELHGKSCAGVDAFPE
                     EVRMSSILTGNDLGRLGSCDHLPSAPEVLKVMKSEMFNRWVIRSQGSRETLISIVHSA
                     ARELIARNEIFEAAGILLASSRWAGKDQA"
     gene            32804..34036
                     /locus_tag="PKI35_05360"
     CDS             32804..34036
                     /locus_tag="PKI35_05360"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX43847.1"
                     /translation="MLKPSIYIALPVMNEMERLPAFMEALRLQTLQEYSLLVCINQPE
                     KWWKDPSQITVCEDNRQAISFLESFKDGRITIIDCSSEGKGWTGKNHGIGWARKVLMD
                     EIAAKALPDDIILSLDADTLFGPRYLESILQNRMNCPSANAISVPYYHRLTGNEPLDR
                     AMLRYEIYMRSFVINLWRIQSPYSFTALGSAIALPVRAYKMIGGMTPKLSGEDFYFMQ
                     KLVKTGTVVNWNEEKVYPATRLSNRVFFGTGPALIKGIEGDWDSYPVYNMHWYDDIQE
                     TYLCFDRLFTEDIPSPLDEFLISVFGSLPWPALRSNFKTKKHFVRACHEKADGLRLLQ
                     YLKSRQKSENLNDDRNLAELLQYLAIHYQSLPAADLNLFDFSASGLSELDAIRNYLEN
                     VESFFRKQHYDEVCKLNS"
     gene            34067..34975
                     /gene="miaA"
                     /locus_tag="PKI35_05365"
     CDS             34067..34975
                     /gene="miaA"
                     /locus_tag="PKI35_05365"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNX43848.1"
                     /translation="MDQLITILGPTASGKTELAARVAHAIGAEVISADSRQVFRDMNL
                     GTGKDLEDYLVDNERIPVHLIDIAQPGTEFSVFNFVTAFNKAFSEITGRGKRALMCGG
                     TGLYLEAVLKGYNMTEVPVNEELRKEWSAADNASLERMLGACKPLHNTTDTVTRDRLI
                     RALEIEYYRREHPQNKPDRDFSGSPVFGLRFDRATERDRITIRLKTRLDAGMVKEVQQ
                     LREKGVPDQRLLQYGLEYRYITQYILGELRYEEMISLLNTAIHQFAKRQMTWFRRMER
                     GGINIQWLRGEDGVNKNIETILQCVD"
     gene            35039..35743
                     /locus_tag="PKI35_05370"
     CDS             35039..35743
                     /locus_tag="PKI35_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43849.1"
                     /translation="MKPVIPAVFLLLAAAMSLFSACEEQIDTAPVISSITLDPDTVRV
                     GEASEVIINASDSDGDQIIYSYSVSGGSIEGYGDTVLWNAPEIYGHYTLKLLVTDGSG
                     NTTTDSAVLVVLPPVNQTSIKGIAAFSNSIDFDLAGANARLYTSYENWNQGKYALSAP
                     VSGFGPIVNFSFDQLLPGTYLLDIWKDNDNSTTRNTGDYYGWYGSGIPENPSLYQIEI
                     PIDSTMTFNLQMYVLE"
     gene            complement(35868..36491)
                     /locus_tag="PKI35_05375"
     CDS             complement(35868..36491)
                     /locus_tag="PKI35_05375"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNX43850.1"
                     /translation="MRRKLVDSLRLKGIHDENVLTAIDLVPRHFFFDSSFLEFAYEDK
                     AFPIGSGQTISQPYTVAFQTELLRVEKGLKVLEIGTGSGYQACILATMGAKVYTIERQ
                     KLLYDKTKSLLSTLKYHINTYYGDGYLGLKAHAPFDRILITAAAPSVPEALISQLKPG
                     GIMVLPVGACDVQIMTRIIKVSEAEAVQENHGTFRFVPMLGERAQDL"
BASE COUNT         9195 a         8085 c         8848 g        10466 t
ORIGIN      
        1 ctcttttcct tacatttgcc ttataaaatc cccatccgtg ctcctggttc ttgccccgaa
       61 aatcactaac cggctcaggt atatctttaa cctgttgctg aatgatttac tgggacttga
      121 aacctgtttc actgcctctt tggacgagtt ttcaacttat gaaggggcta agttttcgta
      181 cggggtaagc ctggaaagtg atgcgctgtt ttttgcggca aaccatttac ttttcgaaac
      241 gggcatttct tcctgcgaag tttcttgttt cgattatgcc ggatccaagg ctttttttgc
      301 cgtttaccag aaatcgtcgg ccatgccgtt cgatgttttc gcagcctcgt ttttcctggt
      361 atcgcgttac gaggagtatc ttccgcagat gcgcgacgaa cacggccgtt tcagggccat
      421 gcaaagtgaa gcgtaccgta acgggttttt acgaaagcct ctcgtgaata gttgggccct
      481 ggaactcaaa aaagtgcttc aggacaggtt cccggaactt gaattcagcc tgccgtcatt
      541 caaattcatt cctacaattg atgtggatgc ggcatttgcc tataaacata aaggcatgac
      601 ccgcgctgtg gggggattct tcaaggcgat caaccgaagc gattaccagg aactcaggga
      661 acggattgaa gtgctcctgg gaaggagggc cgacccgttt gatacctatg aggttattat
      721 ggcgcttcaa aagaaatatt ctttaaaaac gttgtttttc atcctgcttg ccgattatgg
      781 tgtcaacgat aaaaaccttc cggtccaaaa ccggtatttc aggcagatca tacgcgaact
      841 tgccgactat gcagatgtcg gcatccatcc gtcgtatgct tcatccggga atgcagccgt
      901 tcttcagaaa gagatttcaa ggctttcggg tattctccac cgtgatattt taaaaagccg
      961 gcagcatttt ctgcgcctcg aaatgccgac aacctaccgc aacctcatca actgcgacat
     1021 tgccgaagac tataccatgg gttatgctga agaagccggc ttcagggctt ccatctgcag
     1081 cccttacaac ttcttcgatc tcgatcttga ccggaaaact ccgttgcgaa tattcccgtt
     1141 tgccttgatg gacggtacgc tcaacgatta tatggacctt acgccagtgc aggcttcgga
     1201 tctgatctca aacctgatga aggaagtgaa agcggttggg ggaacattca ttagtttgtg
     1261 gcataacagt acactcaacg accagcggca ttggaaagga tggctcatgg tatacgagga
     1321 tatgatcagg ttggccgtga aaggtaaata aagcaatctt tatcgtgata aaatatattg
     1381 aacacggaga aatcaggaag aatcgctggg atgattgtat tgcaggcgct ttcaacgggt
     1441 gtgtctatgc ttattcatgg tatctcgata tcgtttgccc ggcctggggc gccctcgttg
     1501 aagacgatta tctccgtgtc atgccgctgc cctttttcag aaaatgtgcc actgtataca
     1561 gcatgcaacc tgtttttacc cagcaactgg gcgttttttc taaagatgaa cttacacctg
     1621 atcttatcga aaattttatc aaagccattc ctgacgaatt tctcctgatt gactataatc
     1681 tcaatattca caaccagttg cagcccggga attataagct ttttccgatg gctgattatg
     1741 aactgaacat ggctgatgat tatgaaaagc tgaaatctgg ttattctcaa aatacccgcc
     1801 gcaacctgag gaaaggggcc gaaaaccggc ttgtcctttc aacgggcatc aaacctgacg
     1861 atgtggtaaa attgttccga aacggaagcg gaaaagagta taaaaatctt ggcgataagc
     1921 agtatgccac cttgctgcgt ctggtttatc aatgcctctc aagaggaatg gccgttgtat
     1981 ggggcgtata cgacaggcat ctggaacttt gcgcgggggc gatatgggtt tttagtcatc
     2041 aaaaggcgat tttcctgttt tcaggtttgt ccgataaagg acgcctgtca ggcgccatgc
     2101 cattcctgat tgataacttt atccgggaac atgccggcag ccggcttacg cttgatttcg
     2161 agggttcgaa caatgaaggg ctggcaaggt tttatgccgg tttcggttct tccaggctga
     2221 tatatccccg cctcgtcatt aacaggctgc ctttggccgt aaagatcgcc ctgccggttg
     2281 tccgcaggat aaggaatatt ttctgattat tcatttttta tatggtttcc atgttacact
     2341 aaatcagtac ctttgtcggg taatctgcag aaagttttta tttatctcaa acctggatca
     2401 tcatggttat caaccttggg aatcagcaat cgtttgtcag tcaactggtt tcggaattac
     2461 gcgatgccaa aattcagagc gacagcatgc gtttcaggcg gaacctggaa aggatcggtg
     2521 aatttttcgc ttatgagatc agtaaaaagc tgcattatga aaacagggag gtagttactg
     2581 ccctcggaac cgcgaccgtg ccggtgctga aggagttccc gatccttgca accatactgc
     2641 gtgccgggct tcctgttcat aagggattgc tgaatatctt tgataaatct gaaaatgctt
     2701 tcgtatcggc atatcatgat cacagcaaag aggatcagtt tatggtggat gttgaatatg
     2761 tttcatctcc tgaacttacc ggacgaacgt tgatcatttc cgatgccatg ctggctaccg
     2821 gccattcgat gtttcagacg tacaaggctt tgatcggcat cgggaaaccc gctcatacgc
     2881 atattgtttc tgttgtggcc agtgcacagg gtattgaata cctgaaaagt tcgctgccca
     2941 aaaccggcac taccatctgg gtaggagcca ttgacgatga acttactgct cacggatata
     3001 ttgtgccggg gcttggcgat gccggcgatc ttgcctatgg gaaaaggcat gatttttgat
     3061 catcattccg gttgattccc tgttaattaa tttcttcagc ctgcaacatt tccgataagg
     3121 aagcgtttga attactataa aaatcctgca tgacatcttt tgtgaaattt cttctgatcc
     3181 tggctacaag cagcattaaa ttcctatttg caccgccgtt gtcatatggt tttggatttg
     3241 gctttttgca aacctgggct ttcacatcag tgggaggcgt gcttggcgtt ctttttttct
     3301 tcatgctgag caagatcctg atcaatattt atgtgaaata tttcaggaca tcattcaggg
     3361 aaagactact tcaacttgcc ggcagaattg gccagcggca tatcgctgaa cgttatctgc
     3421 cgaagcaaac cagggtgttt acccggcaga ataaaattat tgcatggatc aggagccgtc
     3481 taggtttcac cggcgttatt atcctggcac cggtgttgtt ctccgttccc cttggaacat
     3541 taatcgtaat gaggttatat gccgggaaaa aatacctgcc ggtctatctt tcagccagcg
     3601 tggtggcatg gtcgctggtc atttcaacat caattgccct tttctgacga ttaaccattc
     3661 attcagttga tccctgatgt gacagaacgt tggagaacaa caacgtcacc cgaatggctt
     3721 gtttttaaaa accgtcccat tacctttttt tgtttaattt tacatttcat ttgagatgaa
     3781 gcggatgttt tatccgcatt ggttgtacct ggattatcca tagtaaaaaa tctgcttgat
     3841 ggccagttcg tttttttcgg ggctcgttta cgaaaatgtc cgcatatcgc tgggatctat
     3901 ccgcagccac atgcttcgta ctatcctgac cgtccttatc atagctttcg gtatcatggc
     3961 gctggtcggg attcttactt ctatcgacgc gatcaggtat tacctgaacg aaaatttcca
     4021 gatgatgggc gccaatacgc tcactatccg caacaggact ttacaggtcc atatgggggg
     4081 ggagcgttcc aggccaaaat tcttcaggga aataaccttt gatgaagcca tgcgcttcaa
     4141 acaggaatat tctttcccgg caaatgtctc ggtttttatt cgcgccacag gaactgcaac
     4201 cataaaatat ttatcgaata aaacgaaccc gaatatgcct gtttttggta ccgatgacag
     4261 ttacctggca acatccggtt acgaaatgga caagggacgc aattttactc cttctgaaat
     4321 ttattatggt tcaaatacgg ctatcatcgg aactgatgtc gtaaaacgga tctttaaaaa
     4381 caaggaggac cctgtcggga aatttatcag cattggtccg ggaagatacc tggtgatcgg
     4441 tgtgctgaaa gaaaaaggtt ccagcctcgg ttccaacccg gatgccattt gcctgttgcc
     4501 aatcaataat gtccgccaaa agttttcccg cgcccgcgca agttactcca tcaacattac
     4561 aacaaatgac aactcattgc tggatgctgc cgaaggagaa gctgccgggc tgcttcgtat
     4621 tatccgaaaa caatcggttg gcgaagaaga tagtttcgat atttcgaaaa gtgacaacct
     4681 tgccaatatg ctcttcgaaa atttaaaata tcttcggctg gcagccacta tcatcgggat
     4741 tattaccctt atcggtgcgg ccatcgggtt gatgaatatc atgatggttt cggtgaccga
     4801 acgcacccgg gagattggca tccggaaagc catcggcgcc aaacgccaca ctatccggaa
     4861 ccagtttctg gtcgaagcca tcgtgatcgc ccagatagga ggggcgttgg gtattatttt
     4921 cggaatagct atcggtaact ctctttcgat ggcaatcggg agcagtttta tcattccctg
     4981 gtcctggatt ggcctgggag ttaccctctg ctttctggtt gccctggtat ccggcatcct
     5041 gcctgctacc aaagcggcta atctcgatcc cgtcgattcg ctgaggtacg aataaagatt
     5101 gattattttc ccctggaagt gtaatttccc attccaacca actggtcata agtcgttccg
     5161 cgctcacgct gatacaccag tatcgtatag gtgttctgcg tctgcgaatg attgccttcg
     5221 atataggtaa tgtcgcccgt agcggactgg ttgggcaaaa acatataagc ataatcgtag
     5281 tacccttgtt tgagcatcag ggttgcttca tatccgtggc ggctatagtt gtaatgcatg
     5341 aggttctcat cgttgtattg ccagccgtta aaagcgccgg cgacatatag ctgcccatga
     5401 acaaccggag cgtcataacg taggaacatc cgcacgctgg catattcacc catcgtttca
     5461 accgacgatt catcatcggt tttcaggagg aaccggccgt cgatatcgga attattcaca
     5521 taaaccttga aagtgcgccg ttcattgtcc cataaggtta ccaaataggt cgtatcgttc
     5581 atgttgatac tgcggatatt gcccgaaacc atttttacgc ttttcaggtc aataaaccgg
     5641 aattcgtttc cgccatcaaa ctggtttatg ccgttgtcga acgaataatc aagcacgtcg
     5701 ccccgtacca tgaaaggctt cagggtggta agggagttat cccagcggtt attctgcaaa
     5761 atggtaacat gaatatcctg atatggcgat tctacccggt atccggctga gtttaccgta
     5821 aagtctattt cctgccgcgt ttcgatatct tcaatggttg aagcccgggt aattttcgca
     5881 tcaatgatga atttcggatc tgccaccatg aaccggcgcg tcaggaccac ggtttcttta
     5941 tctttttcct cccgtttaaa aatctttaaa atgtaattac ccgacagcag tatcttcatc
     6001 ttctcgttcg gaaagccgaa ctgataatgg gtgtaatgtt gtaacgtgtt gaacgaaaat
     6061 tgataatctt caatattatc ttcctgtaat ccttcgatgt attcgttagg ccacagatcg
     6121 gaagttttcc agttggcgtc gcaatgaata accgtataca ggtatggttc gatattgcca
     6181 ttcaggtcgt caaattcgag gtgcagcgtt tcaccgctgt taagggttat gacaggcgcc
     6241 gacaattcca catccgattg cctgaatacc accgaccgga tgtttttcag ataaaccaca
     6301 tcttcgtttc ggataaattc cgggctataa taatcggtag tgtccggtaa cgctggtttt
     6361 acttcctgac tgcctgagga tgcaggcgcc gggtaaagag gcccggccat aaggcaggat
     6421 gcctgtatta tggaagtcag cgcaaggaag aaaatggtta tgctgaacct gttcatgatg
     6481 taagattttt acaaaagtaa tagtatttgg gaatgtgaca attctggctg gctattccaa
     6541 aagagaggca aatgtcttat atttgtaata cgtcaaaaaa aaaataaata ttttgcagta
     6601 aagaaggtct gcaaatatct aggaatttat atatttggcg cgaattttca gaattacatc
     6661 gaagtgtagc aagagtgaaa tggatggttt cattaaaata aaaaaaggcc ttgatatcaa
     6721 attgttaggc gaggcgcaaa ataaagttac cgaaattctg accgatgatt ttgccctgaa
     6781 gccaactgat tttatcgggg tatttccacg gctgttggta aaagaaggag atattgtaaa
     6841 ggcagggagc cccgtttttt gtgataaata ccgcgaatca gttcagttta cttctccggt
     6901 gagcggtaaa atttccgaaa tcaggcgggg tgataaaagg gttttactgg aagtaggtat
     6961 ccaggctgat aaggatattg tatttgaaga tttcggaagg gaagatatgg caagtctcgg
     7021 ccgcaggcaa cttatcgata agatgctgtt aagcggggta tggccggcaa tccgtcagcg
     7081 gccctatacc gtgatcgcta acccggacga tactcccgcg gcaatattta tatccggttt
     7141 tgattcggcg ccgctctctc ccgattatgc ctttatcctg aaagatgagc tggccggttt
     7201 ccaggcagga gtagatgtac ttgcccggtt gacaagtggg aaagtgtata ttggcattcc
     7261 ggaattcaat catcccctgg cagggataag gaatgccgta ataacctgtt tcaaagggcc
     7321 gcaccctgcc ggtaatgtcg gaattcaaat tcatcatatc cgcccgataa ataaaggtga
     7381 tattgtgtgg acggtcaatc cgcaggatgt tgtcgttata ggccggctgt tttcgtcagg
     7441 ccgctatgat gcctccgtca ccgttgccct ggcggggtct gaagtgcggg aaccgcaata
     7501 ttacaaggtg cgcaaaggga tttcggtcaa accccttatt tccggaaacc tgaaaggcga
     7561 tacccagcgt atcatcagcg gcaatgtgct cactggcgac aggatcagtc acaatggcta
     7621 cctgggcttt taccataatc agtttaccgt tattcccgaa gggaattact tcgagttcat
     7681 gggttgggcg atgcctggtt ttggtaagtt cagcttttca aagactttct tctcatgggc
     7741 tcataaggat aaaaagtaca aactcgatac aaacttccat ggcggtgaac gatcgtatgt
     7801 gatgacaggc cagtatgaaa aggtgcttcc tatggatatt ctgcctatgc agcttatcaa
     7861 ggcatgcctg ctcgaagatg ttgaactgat ggaaaagctt ggtatctatg aggtggatga
     7921 agaagatttt gccctggtgg agtatatcga cacctcaaaa acagagattc aatccatcat
     7981 ccgcaagggc attgatctga tcaggaagga aatgagttaa tttccggtcg ccggatttcc
     8041 tgagcataca tcaggaaatc aattcatcaa agattcttgt aagcaacgac ttttcaactt
     8101 tacaatttcc ccttttaagg gaattcaggg attaataatg aaagctctac gcaattatct
     8161 cgacaaaatc aaaccaaggt ttcaggaggg tggaaaactc gaaaaattcc attccacctt
     8221 cgatgcattt gaatcctttc tttttgtccc cgacaaaaca acctttaaag ggagccatat
     8281 ccgtgacagc atcgacatga aacgcaccat gacggtcgta attcttgccc tgatgccggc
     8341 cctgcttttt ggtatgtgga atgttggctt ccagcatttc cgcgctacag gacaggaggg
     8401 aggtttttgg cagttcttcg ggtatgggct tttaaaagta ttgcccatta ttgttgtttc
     8461 ctatgttaca ggactcatta tagagtttgc atttgcccag tatcgcaaac acgaagtgaa
     8521 cgagggcttt cttgtgacgg gaatgttaat tcccctggtt atgcctcccg atatcccgct
     8581 atggatggtg gctgtatcga ctgcctttgc agtgattctc ggaaaagaag cgttcggcgg
     8641 taccggcatg aatgtcttca acccggcgct gctggcccgc gcgtttcttt tctttgccta
     8701 cccggccttc atgaccggcg acaatacatg gatagccggg cttgcctcgg gcactggtat
     8761 tgttgatgga tattccggcg caacccccct tgccaatgca gcaaccgaag caatagataa
     8821 aattccaccc ctttccgacc tgtttttcgg cttcattccg ggcgctatcg gtgaaacttc
     8881 aaagcttgcc gttttattgg gcgctgccct cctgctgatt accggtatcg gaagctggcg
     8941 tatcatgctc tctgccctga ccggcggcgt tgtaatgacc ttgattttta atgcgtttgc
     9001 tgcaaacaag ttcatggcca tcgatccgct gacccagatc atgatgggca gtttcctgtt
     9061 tggtgtggtg tttatggcta ccgatcctgt tacggcggct cagacaaata aaggcaaatg
     9121 gatatacggt ttccttgtcg gaacttttgc cattatggtc agggtattca acccggccta
     9181 ccccgaagga accatgcttg caatcctgct gatgaacact ttcgccccgc tgatcgacca
     9241 ttatgtgatt gaagccaata taaaacgcag attgaagagg gccaaagccg ttgtaaaatc
     9301 ctaaacggaa gagaagatat ggaacacagc aataaatata ttttcgttta ctccctggtg
     9361 atggttgtgc tggtagctgt atcgctgacc attgttgccg ttcagctaaa accggcacag
     9421 gatgacaaca tacgcatcga aaaaatgcag aatatactgt cttcggtcaa tgtgccggtc
     9481 gccgaaatac ctaagaaaca ggtgatcgac gtttataaca aatatgtgaa aactgctttt
     9541 gccgtgaatg taaacggcga cgtgacggag acggatgccg aaaaggtgtt taaacttgaa
     9601 atggccaatg aactgaagaa agagccatcg caaagacagc ttccggtatt tgttgccgtc
     9661 ctggataacg gcgattcagc ctacatcgtt ccgttgcgtg gtaaaggcct ttggggggcg
     9721 atttggggat atctttcatt taaaaccgat tttaatacgg taacaggttg tatgttcgac
     9781 cataagagtg agacccccgg tctcggcgct gaaatcaatc agccatggtt ccagaaacct
     9841 tttgccggta agaagatttt tgacgatcag ggccgtttgg tatctgtagg agtcgtcaag
     9901 aaaggacatc caaaggacga tacccatgat gttgacgcca tttcgggtgg taccatcacc
     9961 agcaaaggag ttcaaaaaat gatcaaggat aacctggatg gttatcaggc ttacttcaaa
    10021 aagcatctta aacaatgaac aaggaaaaat taaggaagga aaaagatccc tggttttcgg
    10081 caaagaacct ttcgctcctg aaaaggcctt tgaatatcga aaatcctgtt acagtgcagg
    10141 tgctgggtat ttgttcgtcg cttgctgtta cggttcagat gaagccggcc atcgtgatgg
    10201 ccctgagtgt aaccatcgtc accgcgtttt caaatcttat tatcgcgctc atccgtgatt
    10261 caatcccgtc gcgtatccgc attatcgttc agttggtagt agttgcagcc ttggttatca
    10321 ttgtcgacca gatactgaaa gcatatgttt atgatgtaag caagaaactt tcggtttttg
    10381 tgggactgat catcaccaac tgcatactca tggggcggct cgaggcattt gcgatggcga
    10441 acaaaccctg gccatcattt gtcgacggta tcgggaacgg attggggtac ggcctggtac
    10501 ttgtaacggt agcctttttc cgcgaattgc tcggatctgg aaaattctac ggcatgaaag
    10561 tcattccgga agctttctat aaagccggat atgtggataa cggtttgatg atactccctc
    10621 cgatggccct gataaccatc ggcatcatta tatggataca gcgctggaaa aagcctgaac
    10681 tacaggagaa aaattaaaaa cggaatttca ttttacagca aaaacactga acaatggata
    10741 acttattcaa cctgtttgta agaagcatct tcatcgataa tatgattttt gcatatttcc
    10801 ttgggatgtg ttcctacctg gctgtatcga aaaccgtaaa aacatccctg gggctgggag
    10861 tggcagttac tttcgtacta ctgatcacgg ctcccgtcaa ctggctcatc gatacctata
    10921 tgttaaaagc gggggcgctg gcatggctca gcccttcact tgctgacctg gatctcagct
    10981 accttagttt cctcatcttc attgctgtaa ttgccgccat ggtgcaactt gtagaaatgg
    11041 tagttgagaa attttcacca tcgctctaca atgccctggg catctttctt cccctgattg
    11101 ccgtgaactg tgccattctt ggcgtcgcgc tgtttgttca ggaaagggaa tatacatcaa
    11161 tcggagaagt gactgtttat gccgtcggtt ccgggttggg gtggttactg gctattgttt
    11221 cgctggctgc catccgcgaa aaaatacgtt attcggcaat ccccaaaccg cttcgcggac
    11281 tgggaatagc attcattctt accggcctca tggctattgc ctttatgagt tttatgggga
    11341 tcaagatttg atagccattg aaacgactgt ctaataattt ggaattgtcc gaaaaatgat
    11401 aacatctgta attttattgt tgggtaaact cgccggtccc ttgctgatcg gaacatcggt
    11461 atttctgttt gttactcttt tactggtaac gatgcttctt gttgccaaag cgaaactggc
    11521 cccctcggga ccggtaaaaa tcaccattaa cggagagaag gaacttacgg ttaatgccgg
    11581 ttcaagtctg ctaaccacac tggccaatga aaaattgttc cttccgtcag cctgcggcgg
    11641 cggaggcacc tgcggaatgt gcaagtgcca ggtgcatgaa ggcggaggct caatcctgcc
    11701 aaccgaagtg ggattttttt ctaggaaaca gcaactcagc gactggaggc tcgggtgcca
    11761 ggttaaagtg cgcgaaccgt tgaatatcac tatcccgaaa gaagtgttcg gtattaaaaa
    11821 gtgggaatgc gaagttatat caaaccataa tgttgccact tttataaagg aatttgttgt
    11881 ccggttgccg gaaggtgaaa cccttgattt ccaaagcgga ggatatatcc agatagacgt
    11941 cccgaaaatg gaagtggatt ataaggatat ccaggttgag cctgaatacc aggaagattg
    12001 gaataagctg aaaatgtggg atctgaagat gaaaaatccg gaaccggtat tcagggcata
    12061 ctccatggcc aaccaccctg ccgaaggaaa catagtaaag ctgaatatcc gcatcgcaac
    12121 gccgccgtgg gacagggcta aaaaccagtt catgaatgta aatccgggca tttgctcatc
    12181 gtatatcttc tcacgcaaac ccggtgataa ggttacggtc agcgggcctt acggtgaatt
    12241 tcatatcaaa cccacccgga aagagatggt ttttattggc ggtggagccg gcatggcacc
    12301 cctgcgttct atgatttttc accttttctt taccgagcat accgatcgta aggtcagctt
    12361 ctggtatggg gcgcgatctc tcagggagac cttttacgac gacgagttca gggatattca
    12421 ggaaaaaaat ccgaatttca gctttaacct ggcgctaagc gagccgcttc ccgaagacaa
    12481 ctggaaagga tatacgggtt ttatccacca ggttgtactc gataaatacc tgagcaacca
    12541 tcctgaacct gaagaaattg agttctacct ctgcggtcct cccatgatga acgctgcaat
    12601 tttcaagatg cttgacaacc ttggcgttcc gcccgaaaat atagcctttg atgactttgg
    12661 cggataaatt ctgaattccg ggatgactga aatcggtttt tttagtattc ccgggtccgg
    12721 gaatactcct ttttgctaaa tttcgtttgt taaacggtgg cggaaatcgt tgccattgtt
    12781 taaataaaac tattcctggt atgaaaaaac tgtcagccct tgttgttggg gcaatattag
    12841 cggcctgcgt cctttcgggg tgcaaggcga aaccaaaact tgtgaagttt gtcggtgaaa
    12901 cccaggggac gtattatgcc attacatatt atgcgaagga tggtgtaaat tatcagttac
    12961 agatagactc ccttttacac cggtttgatt cttcggcttc gatgtggaag ccaaattcgc
    13021 tgctatcaag gattaaccga aatgacacga ccgtaagagc ggatgaaaca cttaaaactc
    13081 ttttccggaa atcgatggag gtgtcggagc aaagcgatgg cgcttttgat atcacagtgg
    13141 ggccgctggt agaagcctgg ggatttggct ttaccagcag gatgaaggtg gataagcatg
    13201 ttgttgacag cctgcttcca ctggtgaact acaaaaaact gaaattggta aacgaaaaga
    13261 ttgccaaatc cgatccaagg atgcagatgg atttcaatgc tatagctcaa gggtatgcgg
    13321 tggatctggt gggaaaattc ctggaatcaa aaaatataga gtcatacctc gttgatattg
    13381 gcggtgaagt gctgggaaaa ggaaccaaac ccgaaggcgg tgagtggaac gtcggaattg
    13441 agttaccggc tgccgattca ctggccgaaa ggcaggtaaa agccatcgtg tcgttgcgcg
    13501 acaaggcgtt gtcgacttcc ggcaattaca gaaagtatta cgaagaaaac ggcgtcaggt
    13561 actcacatac tattgatcct gccaccggat atccggtaaa gcacaccctg ttaagtgttt
    13621 cggtgctggc ttccgattgt atgacggccg acgcctatgc tacggtaatg atggttaaag
    13681 ggctggaaga ttcaaagcct ttccttcagg cgcatccgga tgtggaagct tactttatat
    13741 attccgatac taccggacag atgaaaacct ggttttcgcc gggttttaaa aagcttatcg
    13801 aagaagaact gtaatctgca gtaaaaataa gtcccggtca aaactggccg ggacttttaa
    13861 caggaaagtc attcgttgtt tagctgttgt tgcatctggc gctatcgcca ccgcaggaac
    13921 aatccatgcg ctcgccggtt tcaggattcc tgctgctgca cgatttcctg aattcgccgc
    13981 cccggatgaa aaacatcctt atggcaatac cggcgaaagc caatccgacc agaacaatgg
    14041 ccgccagaac aagtttcagc atggtgtcaa cttcttatcc gatggtttta ctggctgatc
    14101 ttgggtttcc tcccgcgacc ggctttatcg cttcccgtcg gaatgtttgt atattgtttg
    14161 tatttcagcc cgatgtagct cttcctgaaa aatacgaaag tattggtttt atcgagtttc
    14221 aggttcgaca ggatattttt gtcggaggta ttacggaact tacatacgta aacagttatg
    14281 tcggaaatat gtttaggctc atcggaagct tcgagcaatt cctttaccag ttcctttata
    14341 gtgccggttt ttactgcttg ccattttttt agggcatacg tgctggtttt tccgatagca
    14401 accacctcgt caatgctgag aatggaggaa cgcagcgatt caatgttcgg cggtatacgg
    14461 gttgtacggt ttttcagttc tttgcatata tccgtgagct tcatcgggtg cccgacatct
    14521 tcaagtattt tgaggatatg ttccgaaagc ttgatgagcg tgtttcggtt gaaagtgatg
    14581 gttgaacggt cgaaaccgag gccaaactct tcggtagcca tacggcggca gatctgtttc
    14641 agcctgtcgc tgccagtttg atgaggatac ataaatttat ttatatattc ctgggcatca
    14701 acctggtatg atttttcaat tcgggtttct ttggtagctt cgatatcggc gaagtatttc
    14761 ttgaaatcaa agatttcctt gtatttgcgg cttaccaggt aataataatt gtaattttcc
    14821 tccaaatcct ggaacgacat gtaataccgc tggaaggttc cggcaaatac aaggcagtaa
    14881 aacttgccgg taaaacttac gccttccagt ttgtttattt tctggacgag gtcgttgtcg
    14941 ataaccagta attctttttt tagatcaagt ccgtaatcga aatgcttttc aatggtggta
    15001 aagcgttgcg aaattttgct gacaatgttt tcgagttttt caggtatttt ctgggcgatc
    15061 tgacgtaccc gctcacgggt gataccgtat tggtctccga tggattgcag cgtacgtttc
    15121 tgtgaattgt tgaagaaccc gaaattgttc atgaatatgt attgctcgcg ttcgtttaat
    15181 acataactga tgagcgtttt caggtatttg aaaaactgga attccttgtt gataaactgt
    15241 tctttgtaaa cttcggtaac ttctgaaggt actttgaagt taacatagca aaacagtttg
    15301 aaatcttcaa aacccagcaa tggttcattc attctcagac tctcaagcgt caatcctgat
    15361 atttcaaggt atttctgcga aagcccgatg agttcaaggt cagtcttgag tccgcaattt
    15421 ctgatatcgc ggaacgtacc acgttcgagg tagtaatata ggattttata aagtgtgtcc
    15481 aaagaaccct caaggcaaac attttttgtc cgctccgaaa ggttgttgat tttttttagc
    15541 tcctcaatgt ggtaattgat gaggtttgat aatggtgcca cggcttttta ttttaggttt
    15601 gacaataatt tgcgaattta gctatttttt agctgaattc agcacatttg cccgaaaata
    15661 atcaagattt tttttcagaa agctgtttgc tgatttcata acagatgatt gcatttgcta
    15721 tggatgcgtt aagcgattct gcagcgctga ttgcgccgtt tatattaaac ggaggaatat
    15781 atattgcttg cgttacaagt ggcagcaaac cggcggaaat tccgtgtgac tcacttccaa
    15841 ttacaacaat tccggcttcg ttaaatgcaa cctgatggag attccttccc tgaagcaatg
    15901 ctccgtacac aggtgtatcc tttggccttt gttttatgaa ttggattaaa tccatttgaa
    15961 agacgcttac ccgggtaaat gaacccatcg tcgattgaat tactttggga ttgaatacgt
    16021 cgacgcagcc gggagaacat ataatccggc gtattccgaa ccagtcagca gtccggataa
    16081 tggtgcccat atttccggga tctttaatgt tgtcaagcgc cagacagtag ctgttatata
    16141 cgtcgggaag catggccggt tcagggacct tagcaagcgc cagcacttta ttgggcgtgg
    16201 tcaggtcgct gattttctcc aggatggttt catcaatttc ggtgaagctg atgcttcttg
    16261 ctttcaaaag acggctgtgt tcctgaagcc agtccggaag agcataaact cctgtgattt
    16321 caaggctgct gtgcagcagt tcatcaacgc atttttctcc ctcaaccaga aaaattccgt
    16381 actgttggcg aaatttcttg atttttaagg cattgatata tttaatctgg tttttcgata
    16441 gcataaaaaa atcccgaatt aattcgggat taaggtagta gaattttacg tattcgtcaa
    16501 cagaaattat tcggcgacaa cttcaaaatt aatgctaacc tggatatcct tgtgcagttt
    16561 gatagctgcc ttgtaggtgc ctaattcttt gatcgattcg ccatcgacat aaattttctt
    16621 gcggtcgatg tcgtaattaa actgttcttt aatggcgtcg gcaatctgta ttgcattgac
    16681 agaaccgtaa atttttccgg tggttgctgc cttggcgcca atttttacag taatgttttc
    16741 cagtttcttt gccagggtcg aagcgtcggc gtttgctttc tgatctttaa aagacttctg
    16801 gcgaattatt tcagtgttca tcttcttact cgactcggtg gccagaatgg caaatccctg
    16861 aggaataaga tagttgcggc cgtatccgtc ctttactgtg atcacatcgt gtgcataacc
    16921 cagagtgggt acatctttca tcaaaataat ttccatctta acctccctta tttaagtaag
    16981 tcagcaacgt atggtaatat agcaaggtgg cgggcacgtt tgatagcctg ggcaactttg
    17041 cgctggtact ttgtactggt gccggtaata cgcctgggca gaatttttcc ctgctcgttt
    17101 acaaatttga gcaggaagtc agcgtcttta taatcgatgt atttgatacc gctttttttg
    17161 aaacggcagt atttcttttt cttgacatca accgttatgg gagtcaggta ttttatttcc
    17221 gaatttcctt gatttgccat gattctttct gtttgagggt ttcaggttag gcctggggag
    17281 ctgcttcggc tgcctcggca ggcttgttgg cgcgtttttt ctcattgtat gcaatcgcat
    17341 gtttgtcgag agcaacagtg agaaaacgca gaatgcgttc atcgcgcttg aaagtggtct
    17401 caagatcggc tatggtgctg ccttcagcct tgaactcgat gagatgataa aacccggtcg
    17461 attttttctg gattggatat gcgagtttgc gcaatcccca gttattttca tggacgattt
    17521 cggccccctt gtccttcagg aatttctgaa atttgcttac cgtttccttc atctgttctt
    17581 cagataaaac gggagtcata atgaaaacgg cttcgtactg attaaccatt tgttttcctt
    17641 tgggttaaat tttaactgtt ttttttaacg gagtgcaaag gtacaatttc ttttcaattt
    17701 acaaacagta aattacttcc attttttgat tctggaaata caaccggcta tatgtaaaac
    17761 tttgcttctt ggccgttggc ttttagctat tagccgttag ctcttggctc tcttggcttt
    17821 tagcgttctg agttttacgt tttgggttct gagttcgcct ccgttctccg ttcttcgtgc
    17881 tcccctgcct gccatctgca ggcttgcgca aggcaggtaa agttttccgt gttccgtttt
    17941 ccgccctcct tgctccatgc tccttgccta cgaaatgctt tcaagcattg tacggcagat
    18001 aaattcagcg gcatgcccgt cgccgaatac atccgggaaa ttcggcgcgg cttccccaaa
    18061 aaaatagtca gccgattcca gtatcctcct ctcatcagca tcagcaagga taccggctcc
    18121 gtttttaatg atttcaaccc attcggtttc gggacgtgcc actaccagcg gctttttaaa
    18181 aaaataagct tctttttgta caccgcccga atcggtaagg ataagggatg cgtttttttc
    18241 gagtgcaacc atttcaagat aggataccgg cggaatgagg cgtatacgcc ggtcatttat
    18301 cagtctgtga taaagatccg gcccgggctg gctttttagt aatctggcag ttctgggatg
    18361 caacggaatc acaatttcca ggtttcttgt cgaagcaatt gttgcaatgg cgctgaaaat
    18421 tgcattcagc cgttggggat tgtcggtatt gttgtcccgg tgaatggtgc acaggacaaa
    18481 cggcttgtcc ttcagggata attcacggag tatggttgtc ctttcttcag ctatcccggc
    18541 gaaataaaca aggttgtcga acatgatgtc gccgcaatgg aatattccgg ggtggtttag
    18601 gctgaatggc gcttcgttgt tggtatggaa accttctttc tgcaggttgc tgatcccggc
    18661 cctggttggt acaaataaaa gcgttgagga ctgatcgcac acgatgcggt tgatttcttc
    18721 aggcatattt ttgttgaagg accgcagccc cgcctcaatg tggacgacag ggacgtgcag
    18781 ttttaccccg gctaatgcgc ctgcaatcgt ggaattggta tcaccgaata gtaccaggaa
    18841 atccggtttc tcttgcatca ggatatcttc aatccccagc agcatcctgg ccgtttgatt
    18901 tccctggctt gccgaaccaa tattcaggtt gtggtggggc ctgggaatat ccagttcctg
    18961 aaaaaaaacg tccgacatgt tcttgtcgta atgctggcct gtgtgaacaa tgatttcttc
    19021 gatttcggca ttgaattgtt ccctgattgc cctgcttaac gcggctgctt tgatgatttg
    19081 cggtctggcc cctactattg taattatctt catgagatgc ggttccggtt gctgtagcct
    19141 caaaatttct ttagtaactg ttttaacctg caattattgt tattacatca ttccttcgtc
    19201 cgcataactg aagtattttt catctccgac aataatatgg tcgagtaccg gaaggtcaag
    19261 aatatcgcct gaacttttca gtttcgatgt aagcttgtta tcggcttcgc tgggttttgt
    19321 cattcccgag ggatgattgt gcccgagaat gatggaggag gcttttgcat ccaatgcaat
    19381 tttaaagatt tttttcggat cggcgacagt tccggcaaat ccgccggtgc ttaccatctc
    19441 cacgttcagc accctgttgg ccctgttcag caggatgatg cagaacctct cctcgtttag
    19501 gtcggcaagt ttttcctgca acaattcaaa agcatcatgg cttgatctga ttgtcctgcg
    19561 gtcaagtatt tctgaactgc gtcgcctttt tcccagttcc agtgccgcag caatggtgat
    19621 tgcctttgct tcgccgatcc ctttgaattt caggaggtcc tttactgata accgggatag
    19681 ttctatgagg tttccctctg ctttgttaag tatttcctgc gctaaatcca atgctgagag
    19741 ttccctggtt ccgatgttta tcaggatcgc aatgagttct gcatcgctta atgcagccgg
    19801 accttttatc agaagctttt cgcggggacg gtcgtcctca agccacaatt tgatgggtgt
    19861 tctgcctgtt tcgctcattt tatctgggat aatttaaacc ggataataat aaagcaccca
    19921 agatacgcaa ataattggtt gtgtatactt tatgacatgg caaaaaaagc ccctgttgtt
    19981 gaacaggggt ggaaatattg taagtgtgat gaattacaac gtatttacct tgtgggcaag
    20041 ctttgattta aggttggcag ccttgttctt gtggataatc tgttttttcg ccagtttgtc
    20101 gatcaaggaa acaatggtag gaagcttttc agcagcttcc gatttcccgg taaggaggcg
    20161 gaaacttttc acagcattac gcatggtttt tgcataatag cgattgcgca gcctcttttt
    20221 ttcgttggcg cggattctct tgatggacga tttgtgattt gccattttta agcgattgta
    20281 atgtttgtag tccgtagggg aatcgaaccc ctgttaccag gatgaaaacc tgacgtccta
    20341 gcccctagac gaacggaccg tttcaaattt tgaggctgca aaggtaatat tattttttta
    20401 attggcaagc atcgggagaa aaggagcaaa aaaaatctca cattcgattt aacagattga
    20461 atatcagttg taaagtgtcg gtttcctcga tgacgcgcat ttttatccgg caaagtattt
    20521 ataaattaat ctcccgtaaa tccggttttt attctccgga agcgaaccgg tcaggaatga
    20581 cagcaaatgg attttccggg ctccataatg atttaacctt ttataacaga gccggatagt
    20641 tcagccagca tctgttcaac ggcatctatt acatcgttgg ttgtaatcag ccgcatacaa
    20701 ttgaaatgtt ttctggggca atggtccaat cctttatggg tacaaggccg gcacggcatt
    20761 tctttttcca ccacgatact tttttccggc aaggggaaaa agcccagttc gcggcttgtg
    20821 gccccgaaaa ttgctgctac aggccttttc tgcgattggg cgagatgcat catcccgctg
    20881 tcgttggtga tgacgagaga acttttattt agcaaagctg ccgattggag cagtgtcaat
    20941 ctgccggcaa aattcgtagc cctgtttttc atcatcgtaa taatgtgtcc gcatagcccg
    21001 gtatcggaag gcccgcctag gaaaactatg aaaacaccgt tgttttgcac caaacggtcg
    21061 gctagctggg caaaccgttc gggaagccat tgtttattgc tgtaggatgc gccgggacaa
    21121 attacaacca ttttttgctg cgggatgaag ccttccggga tgagaaaacg ttcgacagtt
    21181 tgccggtcgt cgtcgctata aaacacttcg gtgccgttgt tgtcatagtt aatgccatgg
    21241 ctctcaacgg cttcaaaata acgcaggaaa acaggtttta cggatttaaa acggttgatg
    21301 cccagtttga tcagtaaggt tcgtaaaagt atgcgcttat gataggatgt tacttctccg
    21361 aaacggacgt tcaaacggat atattttgtt ctcaggcttc tatgaatatc gatgaaccag
    21421 tcaaattgct cccgccttaa tttacgcgct aatacgcgaa gctcatttct cccttttacg
    21481 tcgtcgaatt caattacttc gctgatgtta ggattaaatg agaccagtga tgaaaacttt
    21541 ttctttacca ggaatgtgat tgaagcatcg ggatacgcct gtcgtacaca acgaaccagc
    21601 ggtgttgtga ggataatgtc gccgatcgaa ctaagccgcg cgataagtat tttccgtgga
    21661 ggattcaacg atggatgtta aagggtatgc aagcaatttc ggtgcaaagg taccttttaa
    21721 atgtaatcgt tgatgagtga ctcgagcagt tcctgctttc cgcttgttgt tgccggctcg
    21781 ccgttttctg cagcaagttt ccgcatgtct tcaaggttga gccggccttg ttcgaatgct
    21841 ttgccgtttt ccttgtcgaa tgaggcatag cgttgctttt tcatggccag ataaggagat
    21901 gcagtcagga ttctgtcggc gatgagcagg gcgcgggcaa aagcatccat gccggcaata
    21961 tgggcgatga agaggtcttc gggatctgtg gaattgcgcc ggcgttttgc gtcaaaattg
    22021 atgccgccgt tttggatgcc gccgttttcc agaatcacga gcatggcttc gaccagttca
    22081 taaatattaa taggaaattg gtctgtatcc catccgttct ggtaatcgcc gcggttggca
    22141 tcgatagacc ccagcattcc ggcattggcg gcaacctgca ggtcgtgctg gaatgtgtgt
    22201 ccggccagcg tggcatggtt gacttcgata ttcagtttaa aatccttttc cagcccgtat
    22261 tcgcgaagga atcctacaac cgtggcagcg tcgaaatcgt actggtgttt agtgggttcc
    22321 atgggtttcg gctcgatgag gaaggttcct gtgaaaccgt ttttacggcc atagtcacgg
    22381 gccatggtca ggaaacggcc catgtgttcc agttcgcgct tcatgtcggt attgagcagg
    22441 gtaatatatc cttcgcggcc gccccagaag acataattgg tgccgccgag tgcaatggtt
    22501 gcgtcaatgg catttttaac ctgtgtggct gcccaggcta cgacctggag gtcgggattg
    22561 gtggcggcgc cattcatata tctgggatgt gagaataaat tggcggttcc ccacaggagc
    22621 ctgataccgg tttgagcctg aagctttttg gcatattcca gtatttcgtg cagccgtttt
    22681 tccgattcag cgatggttga gccttcttca acaaggtcaa aatcatggaa acaataataa
    22741 ttgagtccga tttttgtcat gaattcaaaa gccgcatcca tccggttatg agctttttcc
    22801 atggcatcgc ggctctggtt ccatgggaaa ttaaaggtgg ccttcccgaa aggatcgcct
    22861 ccatcgccgc acagggtatg ccaataagcc atcgaaaacc tcagatggtc tttcatgggc
    22921 tttccggcta ccatctggtt ggggtcgaaa tatttgaaag ccattgggtt tttgcttcct
    22981 tttccttcga agggaatttt accgatcccc gggaaaaatt ctgatgttcc ggtataatag
    23041 ttcatggttc tctgatttta tagttctttt aatttttgtt cgagacggtt tttccagtgc
    23101 aaataggtgt ccgacagctt gttgtccttt tccggctgta tttcatccag caccttcagg
    23161 gagttgaaag cttcgccggc attccggtaa aagcctgctc ccaaagcggc tccccgggct
    23221 gcgccctggg cgccgtcggt gttgtataga taaatctcag cgcctgaaag gcccgatagc
    23281 attgtacaga ataacggact aaggaacatg tttgctttgc cggcttttat cttcgaaagg
    23341 ttcagtcccg actgtttcat gatttccatc ccgtaaatga atgaaaatac gattccttcc
    23401 tgggcagcac gtaacaggtg ttccctattg tgaatcgtat aatttaatcc ttcgataacg
    23461 gctccggggt tcttgttttc aagcatccgt tctgcaccgt ttccgaaagg tataattctt
    23521 aatccgtcgg ctcccggctg caccagggcc gcttttttat tcatttctcc gtaactcatt
    23581 cctgccggca tgagcgtgtt tcttatccag gcgttgagaa tgccggttgc attgatacat
    23641 agaagttgac caagccgggg ggcttgcttg ctgtggttta catgaaggaa tgtgttgatg
    23701 cgtgatttcg gatcatattt catccggtcg gttaccccgt aaaccacccc tgaagttccg
    23761 gctgttgcgg ctacttctcc gggatttagt acatttaagg agaaggcgtt gttgggctga
    23821 tcgccggcac ggtatgtaac cgggatgcct ggttttaaac ccagattacc ggcggcttcg
    23881 gctgtcagaa ctccctgtgc cgcaaagacc ggtttaacct cagccagaag gttcggatcg
    23941 atcccgtaat aatcaagcag ctcgtttgaa attgaatttt tggggaagtc ccagaaaatt
    24001 ccttccgaaa ggcctgtttg tgtcgtacag atttcaccgg tgagccgata agcaatataa
    24061 tcgccgggaa gcataatttt gtggatgcgg tcataaacat cgggttcgtt ttctttgacc
    24121 caacggagtt ttgaagccgt gaaattaccg ggcgagttga gaagatggct caggcagaat
    24181 tcatgcccga gttcgccgaa tgctttttcg ccaattttta cggcgcggct gtcacaccat
    24241 ataatggcat tgcgcaccgt ctttccttgt ttatccacgg ctaccatacc atgcatctga
    24301 tatgcaattc caatggcttt tacctgctga agtaatgccg gattttccga cccgatggct
    24361 ttaagggctt cggcaaggtt acggtaccat tcatcgggat gttgttcggc aaatccgggt
    24421 gaaggcgcca gtatttccat ttcggcagga gggtaggccg ccgaagcggt aacgttgcct
    24481 gtatccccgt tgataatgca tgtcttgacg gaggaacttc ctatgtcgat accaagaaaa
    24541 aacatattcc tgttctgaat ttacagtaca aatgtaattg tattggctat tggggaatgg
    24601 tactatttaa gccaattttt ataagaaacc ggctttaatc cgttgcggga atatgtcccg
    24661 gcatgcattt atccttacga tatccggatg gtcaggaacc aaactcttcg tggtcgccct
    24721 ggaacctgaa accaagccgt ttggcggtcc ttatcttggt gctgtcgata gcgctcatca
    24781 tatcgccgac tgaaacaact tttacattgt cgtaaggtgg cgtcagtaag tcgaaattaa
    24841 cggtataccg gactgccgag tcaacgattt gcctggcgct ttcaggattc ataacggcgc
    24901 tctggaagct gttgtatagc aggccgattt cccttttacc ttcgatgaaa taatgatttt
    24961 cgttattgat aaatacccgc ccgatcaggt aacccaggtc gttaagacgg ttataacgaa
    25021 acgagtcggc caggaagttg aagatcatga tcatgccaca gtaagaacgg ttggggtcct
    25081 ggcgcacata aggtgttttc atcacctcgt ggtcacggga gaattcgaag acattgctgt
    25141 gcatcatgaa aatcaggacg tcgctgccaa attttaattc agcttcgaat tcactgatat
    25201 cattgaattc aaagggcacc tccggttcca tcgtgttata tttcgtacgg aattcatccg
    25261 acatcttttt cattatgctt ttaaagcaat tgaaggtttc tgccgtgttc cggtatacct
    25321 gctgtttgaa actgaatttt tttgcaaggc ctccgagaag gtcttcgttg gggaggatat
    25381 ccatcatagt tgcaaagtgt taaaagtcgc ttgagagagg tgagtgattc aaatgacgaa
    25441 aataataatt ttataagggt gacaatctca aacctgattg tttttgtgca attgcatggg
    25501 cgcaacgaac cccgtcgatt gctgaggaaa tgatgccgcc ggcatacccg gcgccttcac
    25561 cgcaagggta gaggtcagca acctgtatgt gttcaagggt atgctcattt cgcgggatgc
    25621 gtacagggga agaagtccgt gattcagtgc cgaggatgat ggcttcccgt gttataaatc
    25681 ctttcatctg gcggtcaaac aggcggaacc cttcacgaag tctcttcgtt actgaaaagg
    25741 gcaataattc aggcaaggct gccggtatta cacccggatg gtaggagcat tcgggcaggg
    25801 tcgaggagaa aaccccatcc acaaaatcgt cgaggcgttg tgccggggct ttctgcgtgc
    25861 ggcctgcagc ctgccagcat gcttcttcaa cctgacgctg gaattccagc cccgcaaatg
    25921 gaccgtgctt tgagaagtgc tgaatatctt cctgttcaat ggaaacgaca atcccagaat
    25981 tggcataagg cgaatttctg cggctgttgc tcatgccgtt gactaccacc tgcccgcttt
    26041 cggttgcagc cgggacaatg gtgccgccag ggcacatgca aaaagaaaat acgccacgtc
    26101 cttcaacctg ctttacaagg ctgtaacttg ctgctggaag aaaagaattg cgtaatgttc
    26161 ccggagagga gtggtattga atggaatcta tcagcggctg tggatgttca accctgacgc
    26221 ccagcgcaaa acttttcggt tcgagttgca atcctttatt atgtaacagt tcgaagatgt
    26281 cccgggctga atggcccgtg gcaatgataa cggctgcgcc gtcgtgacgg tctccgttca
    26341 ggtcggtaac accgcaggct ttgccttttt cgatgatgat atcggtgatc cggcagttga
    26401 aatgaaattc tccgcctgat tcggtaatgg tgttccggat cgcctgtatg atcgcaggca
    26461 atttatcggt tccgatatgc gggtggctat cgataagaat gtcctcgttg gcgccgtgta
    26521 ctactaaagt ttcgagaatt tcgctcacat ttccccgttt cgatgatcgg gtgaagagtt
    26581 tcccatcgct gaaggttccg gctccgcctt ctccgaaaca atagtttgag tcgggattaa
    26641 tcaagccttc cctgttaagc cgggcaatat cggatttccg cgattttaca tctttcccgc
    26701 gttcaaaaat tacgggtttc agtccattcc cgatcagcgt aagggcggca aaaagccctg
    26761 ccggccctgc cccgatgata atcaccggct gctttcgcga tacgtcagga tagtttttct
    26821 ttacaggttt cagggctcca acaggcaatt catcgatgaa gacctcagct ttcagactga
    26881 ttttaatgtt tttggaacgg gcgtcaacag agcgttgtat cggtctgata aactttatcc
    26941 ggctgaccgg gatgcgcagt tgttgtgcaa aaaaatcttt ctggcttgcc ttgtcggcag
    27001 cctggcgagg agtcatccgg atttcaatct ctttgcgcat agatatcctt catgaattca
    27061 ggtgaaaccg atggatccgg gccggctatt gatgaagcat ctgctgaaag gccggaaccg
    27121 tgttttattc aaaagtgaaa accaattgaa aaatcttgga attcaggttc tcaatcccgc
    27181 ccgtatagat ggtcttgtca tgcttcagga cgtcaaacag gccgtatgac atgcgcaatt
    27241 cggttccgaa tttaaaaacc gcattgtaaa aatcgaagcc aacaccgatt tctccataaa
    27301 catcatgctt gtcgagaaac acatgtgtag ccgtgctgtt gtcttttttt ttggcgtttg
    27361 atgcaaggtc aagggagtat ttggcgccgg ctaaaacata aggccttaca ttgtgtatgc
    27421 gttccccctt aaaccggacc catatgggaa aatcaacgaa cgtggattgt atattcttgg
    27481 tcacctcatc caatgtgggc tctgaagatc cttctgagtt ctgaatggaa taagtcagcc
    27541 ccctttcgcc gaatgtcagc gaaggtgtaa accggaggtc gaaatactcg cccaacctga
    27601 ggttactcac aatgcctatc aaaaagccat aggtgggatt ggcaagaacg ccaaacagat
    27661 gagctgtgtc gcaggtgagg tcagggatct ggtcaatatt gctattgttt acataggtta
    27721 cttcctgaaa attctgtttc gtctcaacgg tgaaaaacat cttgttgata cccaactgga
    27781 aaccgaaatg gtaaggcgac tggtcaaagt tattcagatt gggtactttg gtctgggcag
    27841 ataaatcgcc tatgctgcac aacgccaata tggtgataag aggtaatata gtccgatagt
    27901 ttttcaaggc aggcaaattt tctttagtac tcaaacgaac gcgcttcgga tttagtattc
    27961 cgttcaatag cggatgcaaa agtaaggaga ttgccttttt taaccatttt ttctgaatcc
    28021 ggtatacagg ctggcaattc cgaaagttag ttttcgggct gaaacattta taaaaccgat
    28081 gtctttcata atggaaagga accgatcacc ctgcggaaaa acaatgacag atgcgggtaa
    28141 ataagtatag gcggacctgt gttttgaaac gaaacttcct aacgcaggaa gtatgtaatt
    28201 gaaataaaaa tgatatactt gttttacagg aaattgtgtt ggcatagaga actcaagaat
    28261 gtaagttcca ccgcctggct tcagaacccg gtgcatttcc gaaaggcctt ggttaaggtt
    28321 ctcataatta cggacaccga aggcaaccat ggtcgcatcg aaggcgcaat cggcaaacgg
    28381 aagtttttcc gaatctcccg gcagcagttt aatgatattg ccaagccctt tccttttaac
    28441 tttttcattt ccgatattaa gcatttcacc ggcaatgtca attccggtaa tgttttccgg
    28501 gttcagcacg gatgccgcaa tggcaaggtc ggcagttcct gttgccaggt caaggatgtt
    28561 ccggtgagga tattgtgcca gggtttttac cagcgtctgc cgccataggc ggtcgatgcc
    28621 ggctgaaaga aaatgattca ggaaatcata acggggggca atgtcgttaa acatcgtctc
    28681 tacctgttct tttttgcctg ctgcctgtat cacggtaagt tcgttgattc gccgggatat
    28741 ggtttcccgt tttgtatgac tgggaaaaat gggaaggttg ctaaataagc gactgggctt
    28801 ccctgatcag tttctgcctg ttaacaggga ctacgcccac ttcctcacaa accagtccgc
    28861 cggcaaggtt cgatatccag gcggttttat cgggagcaat tccaagcgcc atacaaagcg
    28921 aagccacgct tattaccgta tcgccggcgc cagatacatc ggaaacattt cgtacatggg
    28981 aaggcagaat gaaggtgtcc tccgtgccat tgaaccggct gctcatgaaa accccgtttt
    29041 ccgataaagt gatcattatg tgctttgcat ccagcagatt gtgtaatttt ttggcggcat
    29101 tactcaagct ggatttgtca tagggatcga tatccgtatt cagggcttcc ctgatttctt
    29161 tcagattggg cttgaaaaga gtggctgaac ggtatgctgt gaaattcttc tttttcgggt
    29221 caaccacaac cgggatgcct gcctgcaaag ccttgatcat gatcgtatca atcacgccat
    29281 gagtaaggac gcctttatta taatcctgca ggacgattac cgccggtttg gcctggtata
    29341 acagctcttc aaaccggaga agcaatgcat cgctgtcgct tttcgtgagg tcgctgtcga
    29401 tttcctcgtc aaccctgatc atctggaacc tgtttccgat aacccggaat ttggtagttg
    29461 tgatgcgctg actgctttga atgattccgc ctgtatcaag atcgttgccg gtaagcaaat
    29521 caatcagttc cttgccgtgt ggatcgttac cgattactga acacaggatg gggtttgctc
    29581 ccagcgattt aatattcagg gcaacatttg cagccccgcc cagcatgctt tcccttttct
    29641 gaagggcgac gacgggaaca ggggcttcgg gcgaaatgcg gtcaactttt ccgaaaaggt
    29701 aagcatcaac catcacatcg cctacaacca acacgttcag gttagcaaat cgctcaaaaa
    29761 gatcgctgat ttcttcccgg tttttaaaca ttctttccag ggtgaaaatg gttttaagga
    29821 taattaactg agtttcgaca acgcggtttc aatacgcctg atggcttcct gaaggttatc
    29881 ctccgaggtg gcatacgata tgcggataca gttgggggtg ccgaaagccg agccgggaac
    29941 cagagctaca tacgccgtgt ccaggatata atcgcaaaga tcggcgcccg aatttatcac
    30001 atggcttccg tttgatttcc cgaaatattg actgatttca ggaaacaggt aaaatgcacc
    30061 atcgggtaca tttaccgtga tcccgggtat ttgtttgagc atgctgattg ccaggtcgcg
    30121 acgatattta aaagcatgga tcatggattt gatatcttcg ctttcagccg gatcggtctt
    30181 catggctgta atggctgcac gctgggagat tgagcaggtt cctgaagtaa attgtccctg
    30241 cagtttatca caggctttgg caatttcacg acaagcggcc aggtatccca gcctccagcc
    30301 tgtcatggca aaaccctttg aaaggccgtt aatgatgatt accctgtcgt ggatgctccc
    30361 gaacgaggca atgctgtaat gttttcctgt aaaattgatg tattcgtaaa tttcatccga
    30421 gatgatgaaa atattcggat ggcgttcgaa aacttcagca aaagctttca gctcttcatg
    30481 cgtatatact gttccggagg gattgcaagg gctgttgaaa ataaacaatt ttgttgaagg
    30541 tgtgatggca gcttcaattt gtgacggcgt aaccttgtaa tcgctggcaa tcgtggtatt
    30601 gatatagacg gcttttgctt cggcaagctt gatgagttct ttgtatgaaa cccagaaagg
    30661 ggcgggaaca ataacttcat cgccggggtt gagcaggcag agaatggcgt tggccagcgc
    30721 ctgttttgcc cccgttgaca ccacaatctg atcggcttta tagtcgaggt tgttatcacg
    30781 ttttagcttg agcgcaatgg cttcacgcaa ttcagggtaa cctggtaccg gaggatagtg
    30841 ggaaaaattc tggtcaatgg cttcttttgc cgcgtctttt acatgttccg gggtattgaa
    30901 atcgggttcg ccgatgctaa gattgataac atcaaagccc tgagctttta attcgcggct
    30961 tcggcgcgtc atggccagtg tttccgattc agcaagagcg ttgatgcgat ccgataaaaa
    31021 gttattttcc atgtcttgtt tatttctgtt tcatttcaaa tgacaaaagt agaaaacttt
    31081 attgtttgaa aaggctgtaa agcggtttct tgttaattgt ttcacaaaag catgatctga
    31141 ggcaggttgc cgtctgaggc tcgggaaata taaaactgtt gaagctgaaa atggtgtatt
    31201 tttgcgacgt tgccaacata tgaaagcact ttaaaaattt tgtcatgaac cttatccttc
    31261 agctttccca gatttttctt cctgttatcg cggtcctggc agtcacctat tacctggtga
    31321 aaagattcct cgatcatcac accgaacaac aggagatgaa tatgaaaatg aaattgcacg
    31381 aatcggttac cccaatccgg ttacaggctt atgaacgcct gatgctgctt ttggagagga
    31441 tgcagccggc cggtatgctc atgcgtcaca accgctcggg tatgaacgcc attgaacttc
    31501 aaacggccct gcttcagggt attcgtgatg agtttgagca taacctttcg caacagctct
    31561 atgtttcaat tcaggcctgg gaaaaagtac gcaatgcacg tgaagaaatg ataagcctta
    31621 tcaatacggc tgctgccaaa gtcaaacctg atgctccttc ggctgatctt gcacaaaatg
    31681 tccttgcatt atctctggag gttgaaatac tgccggtaca gcaagctatt gattttttaa
    31741 aaattgaagt caggcaaaat ttcttttaaa ctggcaggat gtaaaattta tcctcataag
    31801 gttagatttt cggcaacatg ataacgtata accccgccac cctgacgccg atacagatgc
    31861 tgcggctgct gcagggaacc gtcatgcccc gccctgttgc attggtaagt actttgaatc
    31921 gtcagggcta tccggccctt tttccttgca gctactatac tattgccggc tctgatccgg
    31981 gaacgctggt tttttcacca gtccggaacc ggaaagatcc ttccgcaaag gatacaatcc
    32041 tcaacataaa agaaattccg gaagtggtca tcaatgcagt atcttaccgg atggttgagc
    32101 aggcaaacct ggcaggccat gaatttgccc gggcgacgga tgcttttctg aaagccgggt
    32161 ttaccgctct tccttccgaa aaagtgcgtc cgtttcgcat acaggaatcg cctgttcatt
    32221 ttgaatgcgc cgtaaaacag gttgttgaaa ccggtaaacc tggtgcggca gacatgctga
    32281 taatatgtga agttctgctg atacacatag atgattcggc gatcgacagc cagggccgca
    32341 ccaatcccga caaactggac gtggtaggcc gcatgggagg ggatgtgtat gtgaaagcat
    32401 caggaaaagc attattcagg gtccgggaat tgcatggtaa aagttgtgcc ggcgttgacg
    32461 cttttcctga agaggtacgc atgagcagta tccttacggg aaacgatctt ggaaggctcg
    32521 gtagctgcga ccatctgcct tcagcgccgg aagtgctgaa agtgatgaaa tcggaaatgt
    32581 ttaaccgttg ggtaatacgc agccagggaa gccgtgaaac actgatctcg attgtacata
    32641 gcgctgcacg ggaactgatc gccaggaatg aaatctttga agcagcagga attctcctgg
    32701 cttccagccg gtgggccgga aaggatcagg cctgatccgg actataggag atcaggttct
    32761 gaggtaaaca ataaaaatag agagatatac cgcttgttat tgaatgctga aaccatcaat
    32821 atatattgct cttcctgtaa tgaatgaaat ggagcggctt ccggccttta tggaggcgtt
    32881 aaggttgcaa acgttacagg aatactcgtt gcttgtatgc atcaaccagc cggaaaagtg
    32941 gtggaaggat ccgtcacaaa taactgtttg cgaagacaac cggcaggcta tcagttttct
    33001 ggaatctttt aaggatggcc gtatcacaat cattgactgc tcatcggagg ggaaagggtg
    33061 gacagggaaa aaccacggta tcggttgggc gcgcaaagtg ctgatggatg aaattgccgc
    33121 taaggctttg ccggatgata tcattctcag tctcgacgcc gatacgctgt ttgggccgcg
    33181 ctaccttgag agcattctgc aaaacagaat gaactgtccg tcagcaaatg ccatatcggt
    33241 gccatactat cacaggctta ccggcaatga accgctcgac cgtgccatgt taaggtatga
    33301 aatctacatg cgaagcttcg tcattaacct ttggaggatt cagagcccgt atagtttcac
    33361 agcgcttgga tcagcaatcg ctctcccggt gagggcttat aaaatgattg gcggcatgac
    33421 cccgaaactc agcggcgagg atttttactt tatgcagaaa cttgtaaaaa cgggtactgt
    33481 tgtgaactgg aatgaagaaa aagtttatcc ggcaacccgc ctgagcaaca gggttttctt
    33541 cggcaccggt ccggccctta taaaagggat tgaaggcgat tgggatagct atccggttta
    33601 taacatgcac tggtatgacg atattcagga aacatacctt tgttttgaca gactgtttac
    33661 cgaagatata ccttcgccgt tggatgaatt cctcatatcg gtctttggct cgttaccctg
    33721 gccggcttta aggtcgaatt tcaaaacaaa gaagcacttt gtaagggcgt gccatgaaaa
    33781 agctgacggg ctgcggttgc tgcaatacct taaaagcagg cagaaatcag aaaacctgaa
    33841 tgatgacaga aaccttgcag aattattgca gtatcttgct atccattatc agtcacttcc
    33901 tgcggctgac ctgaatctat ttgatttttc ggcttcaggc ttatctgaac tggatgccat
    33961 tcgtaattac ctggagaatg ttgaatcgtt tttccgtaag cagcattatg acgaagtttg
    34021 taaactgaat tcgtgatact tttatactgt cattatcctt ttcgtgatgg accaactgat
    34081 cactattctg ggcccgacgg cgtcggggaa aactgaactg gcggcccgtg tcgcgcatgc
    34141 tatcggcgct gaggtgatca gtgcggattc ccggcaggtt ttcagggata tgaacctggg
    34201 tacaggcaaa gacctggaag attacctggt tgataacgaa cggattccgg ttcacctgat
    34261 cgatattgca caaccgggaa ccgaattttc ggtttttaat ttcgtcacag cttttaataa
    34321 agcattttct gaaattaccg gcaggggcaa acgtgccttg atgtgcggtg gtacaggcct
    34381 ttatcttgaa gcggtcctga aagggtacaa tatgacggaa gtgccggtta atgaggagct
    34441 tcgcaaggaa tggtctgcgg ccgataatgc ttctctcgaa aggatgctgg gcgcatgtaa
    34501 gcccttgcat aacactaccg ataccgttac acgggaccgt ttgatcaggg cccttgaaat
    34561 cgaatattac aggagagaac atccgcagaa taaacccgac cgggattttt ccggctctcc
    34621 tgtctttggg ctccggtttg atcgtgcaac cgaacgtgat cgcatcacaa tacgtttaaa
    34681 aacccgtctg gatgccggca tggttaagga ggttcaacaa ctaagggaaa aaggggtgcc
    34741 tgatcagcgt cttttgcaat atggtcttga atacagatat ataactcagt atattttggg
    34801 tgaattgcgt tatgaagaaa tgatctcgtt gcttaatacg gcaattcacc agtttgctaa
    34861 acgacaaatg acgtggttcc gtcgtatgga acggggaggt attaatatcc aatggctcag
    34921 gggagaagac ggggtgaata aaaatattga aacgatatta caatgtgttg attaaaagat
    34981 tatttattac ttttgaaaat tattcagaat ctgtattcat caagaaactc cttttattat
    35041 gaaaccagta attccggccg tttttttgct gcttgccgct gcaatgtcat tgttttcggc
    35101 atgcgaagaa cagattgata ccgcaccggt aataagttcg atcacattgg atcctgatac
    35161 ggtcagggta ggggaagcat ccgaagttat cataaatgct tcggacagcg atggcgatca
    35221 gatcatttat tcctattctg tgagcggagg atcaatagaa ggatacgggg ataccgtgct
    35281 ttggaatgca cctgagatat atggacatta cacgctgaag ttgctggtta ccgacggatc
    35341 cggaaatacg accactgaca gtgccgtact ggtggtgttg cctccggtta accaaaccag
    35401 tataaaaggc attgccgctt ttagcaactc aattgatttt gaccttgccg gtgcaaatgc
    35461 acgcttatat acatcgtacg aaaactggaa ccagggtaaa tatgccctgt ctgcgccggt
    35521 cagcggtttt ggccctatcg ttaatttttc attcgatcag ttgctgccgg ggacctatct
    35581 gctcgatatc tggaaggata atgataacag cactaccagg aataccggcg attattacgg
    35641 ttggtatggt tccggcatac ctgaaaatcc gtcgctctac cagatagaaa tccccattga
    35701 ttctaccatg acattcaacc tccagatgta cgtgctggaa tagcgcctcc ggtggagtcg
    35761 tcgtttaatc cttgaattcc atactacctg aatttaacaa cccgaaagct agcggttgcc
    35821 ggtttttatg ccccggggaa agggttgatt tgtaaaactt ttcaggctca gaggtcctgg
    35881 gccctttctc ccagcatggg aacaaagcgg aatgttccgt gattttcctg aacggcttcc
    35941 gcttccgaca ccttgatgat cctggtcata atctggacat cgcaagcgcc aacaggcaat
    36001 accattatac ctcccggctt gagttgactt atcagggcct ccggtactga aggggcggcg
    36061 gcagtgataa gtatccggtc gaacggggca tgggccttta atcctagata gccatccccg
    36121 taatatgtat tgatatggta tttcagtgta ctgagcaggc tttttgtctt atcatacagc
    36181 aatttctgcc gttcaatggt gtaaactttt gcacccatgg tagccaggat gcaagcctgg
    36241 taaccggatc cggtgccgat ttccagtact ttcaatcctt tctcaacgcg caacagttcg
    36301 gtctggaaag cgactgtata gggttgcgag atagtttgcc ctgatccgat cggaaaggct
    36361 ttgtcttcgt aagcaaattc aaggaatgaa gaatcgaaaa aaaagtggcg gggaaccagg
    36421 tctatggccg ttagtacatt ttcatcatgg attcctttca ggcgtaatga atcaacgagt
    36481 tttctcctca ttcccttatg acggtacaaa tcggtaagtt tgttggcagg catgttttgt
    36541 atgtaaacag tttcggtcgg cgaaattaca tattaacgat aaagaagcca actc
//
LOCUS       DAOPYV010000028        35733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_1078_length_35733_cov_12.836734, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000028 DAOPYV010000000
VERSION     DAOPYV010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 35733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35733
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1078_length_35733_cov_12.836734"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            597..2408
                     /gene="uvrC"
                     /locus_tag="PKI35_05380"
     CDS             597..2408
                     /gene="uvrC"
                     /locus_tag="PKI35_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNX43851.1"
                     /translation="MPPKTVHLENIVRDLPDRPGVYQYYDKDGKLLYVGKAKNLKKRV
                     SSYFTRDSSLTGKVKILVQKIADIRFIVVDTELDALLLENNLIKQYQPRYNVMLKDDK
                     TYPWICIKNEPFPRVLPVRNLVKDGSEYFGPYASVRMMNTLLDLIRQLYPLRTCTLNL
                     SESNIRQNKYKVCLEFHLGNCKGPCEGLQLQDEYLETLNGIRQIIRGNIASVRQGLII
                     LMKQAAAGYEFEKAQFLKEKIELLDRYQSKSAVVNPSIHNVDVFSYCDDLSAAYVNFM
                     KVINGCIIQSHTIELKKKLDESPAELLSLAITELRERFQSDAREIIIPFAPDVELQDI
                     TTTIPLRGDKKALLELSERNAKYYRLEKNKQLERVDPERHTKRIMEQMQSDLRLKEQP
                     LRIECFDNSNIQGAYPVASMVVFTNGKPDKQEYRHFNIKTVEGPDDYASMEEVIYRRY
                     KRVLEEGKPLPQLIVIDGGKGQLGSAMTSLGKLGLRGKIEVIGIAKKLEEIFKPGDPI
                     PLYIDKKSETLRIIQQIRDEAHRFGITHHRKRREKGTIKTQLTEIEGIGMATAQSLLR
                     KFRSVKNIGNASLEELQAAVGKAKASVVYEYFRSQNQ"
     gene            complement(2494..4077)
                     /locus_tag="PKI35_05385"
     CDS             complement(2494..4077)
                     /locus_tag="PKI35_05385"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNX43852.1"
                     /translation="MKRTVLVILALLMLAAASRAGGIVTNANQSAAYVRMLCRDASTG
                     IDAVYFNPAGLMLLKKGFHLSVSNQSIWQDRNIKSDYLNLNNKEYIGKIVAPVFPSVY
                     AAYKFDRFAVSFGFNPIGGGGGAVFDKGLPSFEYSISDLVPSLQSSGVTGYKTDIYFK
                     GRSVYWGAQLGVSAEVNKNVSIFGGFRVIAANTTYEGYLRNNTLIMGSTEIPAVDFIE
                     IIRQKYLTGTQQATAAIGQFSQYPADLVMPNDVAASAGLPAGTTFGQAVAIMNQTVQT
                     CNVGAAKAQATKTLLSDQEADVTQKGSAITPIFGLNVNLLDQKLNIGFKYEMPVHMKV
                     TNNTKKDVLGGFAPDGSEITMFPDGESFYNDMPAMMSIGAAYNVSDKFLVSAGFHTYW
                     DEAADYGKKLSGEYVDNTDLIDHNFREYALGLEYKIGKRWLVSAGYLLAQTGVKETYQ
                     TDLSNSLTSRSGACGLAVNITPDFQLNVGAMYSVFKDADKNMTHMLGSTAVPVVETYD
                     KDNMILAIGADIHFGCKGE"
     gene            complement(4351..5049)
                     /locus_tag="PKI35_05390"
     CDS             complement(4351..5049)
                     /locus_tag="PKI35_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNX43853.1"
                     /translation="MELKKSAKADLESKKTVFLQIGLIVALAITLAAFEYKSYDKAVV
                     EGYDTKNFVDVPEEMVQITQQQNTPPPPAPPATTTVINIVNNDAIVDDDFKIDAEADE
                     NTQMQQYTAPVKVTTQVVEEEVAEAEIFTVVEESPSYPGGDEARINFLRENMKYPQMA
                     RESGISGTVFVTFVVERDGSVTDVKVLRGIGGGCDEEAIRVIKAMPKWNPGKQRGKPV
                     RVQFNLPIRFTLAN"
     gene            complement(5285..5665)
                     /gene="gcvH"
                     /locus_tag="PKI35_05395"
     CDS             complement(5285..5665)
                     /gene="gcvH"
                     /locus_tag="PKI35_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNX43854.1"
                     /translation="MNIPENLLYTNDHEWILVNGNEAIVGVTDFAQHELGDIVYIEVD
                     TVGEKVAMGETFGTIEAVKTVSDMLIPVDGEILEFNEALVNKPEVVNSDPYGEGWIVK
                     IRIDNASQLGDLLKPADYQKLIEA"
     gene            complement(5795..13078)
                     /gene="sprA"
                     /locus_tag="PKI35_05400"
     CDS             complement(5795..13078)
                     /gene="sprA"
                     /locus_tag="PKI35_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNX43855.1"
                     /translation="MKNLVKYILRLALLAFVILVSWNTSPGRGNAPGEQSGGHFVNPP
                     DSTEGNDTTLRFPIRQSINPYDQLQNGGLYLKDPSNLKEEIIYDPILNEYVLTRKIGN
                     LNYTRPASFSFDEYKDWDLKQSVERYWQEKISAGGVGGRQGIIPQLRIGGELFDRVFG
                     GNTIDIRPQGSAELVFGIVSNRRDDPAIDTRLRRTTNFDFQERIQMSVLAKIGDKIEF
                     NTNYNTEATFDFENKLKLKYEGKEDEIIQLIEAGNVNLPLNSSLITGSQSLFGLKTQL
                     KFGKATFTTVFSQQQSQVENISVQGGAQTTDFSLRSLEYEENKHFFIGQYFRNHYDEA
                     LKTLPVITSNINITKIEVWITNIGAAVTENRNIVAFQDLGESDPYNSHINPLSLNPYP
                     SNYNNDLLNNLDTARVRNINKVNEYLTGVKGYVPGVDFEKVESARKLNSTEYTFNSKL
                     GFISLNSSLNPDQTLAVAFQYTVIGSDSVYQVGEFSDQGIATTKNLMVKLLKSTAVNT
                     HIPMWNLMMKNVYNLGAYNVNSEDFILNVLYSGNSNAVPTGYFTSGPDNIKGVPLIQL
                     LGLDRLDPQSNPPADGIFDFIGNAATSGGTIQSSNGRVFFPVVEPFGSYLRNLINDKT
                     IADKVCFDSLYTMTKTGAQQFPDKNKYILEGQYKSESGSDISLNALNVPQGSVTVTAG
                     GIPLVENVDYTVDYTLGRVKIINQGILNSGTPINISLENNSMFNVYSQTLMGTHLDYK
                     VNKNLLLGGTLLNLYERPITQKTNYGDEPISNTIWGGSVNYSKESGLITKIVDKLPFL
                     STNAASKLRIDAEFAQFLPGHSKAVGKTGTSYIDDFEGAKSTIDLRNVGAWFLASTPQ
                     GQTIRTLFPEAAPGTGLAYGFNRAKIAWYTIDPLFYDNSSNLRPKNVTKEEISKNSVR
                     VVLQNELFPRKESETGQPTNIQVFNIAYFPEERGPYNYDVEASTFSSGINEDGTLKNP
                     ASRWGGIMRRVETSDFDAANIEYITFWLMDPFADDPSNKGGDLYFNLGDVSEDILRDG
                     RKSFEHGLPTSEVKENVDTTIWGLVPTMQAITASFVSDDAARPYQDVGYDGLSTSDEK
                     QFFSSVLLNKIAAKWGITSQAYQDADKDPSSDNYHYFRGSDYDNEDKYSSLVERYKNY
                     NNPDGNSPTPAMNPESYQTLATTTPNMEDINSDNTLSDQERYFQYVIHLTPSGLNVGE
                     NYITDKLEASYQIDDKHSGKVNWYQFKIPIQSPDKVVGSIQDFKSIRFMRMFLRNFEQ
                     PVVCRFGSLELERGEWRKYYNSLLSPGEYIPDPNQDATAFDISSVNVEENYNRANINY
                     LTPPGIKREINAQSTNYYELNEQAMVLKVGNLMDGDARGAYKTTDFDFREYKRLKMFV
                     HAEKTAEAESHVNGDLTVFIRIGSDFTNNYYEYEVPVEYTPWGRYTDTEEGQRSVWPL
                     SNEFNIELDKLVEVKKRRNAAMENSGSAVSVSLPFTITDGNNNITIVGSPSLSDVRAF
                     MIGVRNPKRDSSFDNTDDGQSKNVEIWVNELRLTDFNEQSGWAANARISANLADLGNV
                     VVSGSASTPGFGSIDQKVSERQQESIRQIDFATNLELGKFLPEKSGIRIPFHYDYSKS
                     VTNPQYNPYDPDIKLNEQLNSLSKEDRDSLRAITSDVVQRQNLNLMNVRKDRVGNTKK
                     MHLWDIENFDLSYAYSEINTHNADIEKDIKKSYRGSIGYNFSANPKNVRPFQKIVKSK
                     SLKMLSEFNFYYMPKLLSIRSEMYRTYEERLLRNKSEALIILEPTYTKNWDWSRSYDF
                     KYDFSQSLKFDYNANAQAYVDEPAGGVNRRDDSYQAYRDSVMQSILGFGSMNTFNQTA
                     NLNYMIPFSKISLLNWLTGSARYGAQYRWDASPQSLQARYGNTIENSRNLQLNGNARM
                     TSLYNKVPFLKKALDYNQKLGSPQKPAPPARKGMEREGREKEDVKKQAEKASDADTTE
                     NKPKVDYLRIIGNQALGVLMAFKDASVTYNQTNGMTLPGFKPVSGPLGNNWKDDAPGL
                     GFIFGSQQDIRMVAESRGWLSTDTLLNNPYIDRATSSLTGRANLEPFRNMKIELTADR
                     SYSSTYQGYWKAGSDGKFDMTSAQERGNFSSSYIIWPTAFIRDNKANENATFQQMLEN
                     RNIIAGRLAEKNPNSAGINDTTGFPQGYGATQAEVLLYSFLTTYSGRNPSSASLKAFP
                     AIPLPNWRLTYNLHSGVKAIRDHFQSFNITHSYRSAYSIGGYNTNVAYQENENLPGFA
                     SAMDDLGNFIPQKYMEVVTLTEQFGPFLGVEMTMKNSLMTRIEYKKTRNLSLSFVNNQ
                     LTEVRSNELVTGVGYRFKNIRFNVRSVSGGPKRQLKSDLNVKLDVSVRDNKTVLRRIE
                     ENNNQISTGSMQVSINASADYMVSQKLTFRLYYESTITKPHVLSQIPTSTTNAGISLR
                     FTLTQ"
     gene            complement(13193..13777)
                     /gene="ruvA"
                     /locus_tag="PKI35_05405"
     CDS             complement(13193..13777)
                     /gene="ruvA"
                     /locus_tag="PKI35_05405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNX43856.1"
                     /translation="MYAFIEGKIADLNPAGLVLNCNGVGYQLNISLNTYSKLNGKENC
                     RVYTHLVVREDALVLFGFADEAERSIFQLLISVSGVGPNTARLILSALTPDEITDAIV
                     SENVKALQSVKGIGSKSALRLIVDLKGPLSRESVTKEIFGAKHNTAKVEALSALVMLG
                     FNKAAADKALEAVVKQDGLSLPVEQMIKNALKIL"
     gene            14081..16396
                     /locus_tag="PKI35_05410"
     CDS             14081..16396
                     /locus_tag="PKI35_05410"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNX43857.1"
                     /translation="MKSLLQKNALSYHADGRPGKIEVIPTKPHASQRDLSLAYTPGVA
                     EPCLEIKAKPDDVYTYTAKGNLVAVISNGTAVLGLGDIGPEAGKPVMEGKGLLFKVYA
                     DIDVFDIEVNEKDPERMIATCKAIAPTFGGINLEDIKAPECFEIEERLKAELDIPVMH
                     DDQHGTAIITSAALLNAITIIGKRIEDLKIVVNGAGAAAVSCTRLYVKLGARRENIVM
                     IDSKGVLNKKRTDLNRYKMEFVTGRDIDTLEQAIEGADMFLGLSVAGALKPEWLLKMA
                     PNPIVFALANPVPEIAYDVAHATRNDLVMATGRSDYPNQVNNVLGFPYIFRGALDVRA
                     RAINDEMKLAAAFALAELAKLPVPEEVNIAYNTTNLRFGKNYIIPKPCDPRLIMYVAQ
                     AVAKAAMETGVARKPVTDWNAYETELGKRLGLVNPLIRFIRDKARNEPKRVVFAEAEH
                     YKILKAAEIVKDEGIGFPVLLGDKDKIGQIIKEYQLELNGVPIIDPKSDEQKENRRIF
                     AEQYYSRRMRRGTTLQAALDRMMHRNYFGPSMVENGLADAMISGLTTEYPETIRPALR
                     IIGKKPGVRVISGMYIMLTKKGPIFFSDTTVNINPTVETLVEITLETAAAVRTFNIEP
                     RIAILSYSNFGSVEGDVPLRVRETVSRLHQEYPDLIVDGDMQANFAMNEELLAEHFPF
                     SKLADGKGPANTLIFPYLTAGNIAYKLIQELGGAEAIGPVLNGLNKSVHVLQMGSSVN
                     EIVNMTAIAVIDAQTHGPHGAIAHECSGMPA"
     gene            16547..17488
                     /gene="mdh"
                     /locus_tag="PKI35_05415"
     CDS             16547..17488
                     /gene="mdh"
                     /locus_tag="PKI35_05415"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNX43858.1"
                     /translation="MRKITVIGAGNVGATVANVIAHKDLAREIVLVDVKEGIAEGKAL
                     DIWQTSSINNFNTRVIGVTNDYLRTKGSGIVVITAGFPRKPGMSRDDLIKTNALIVKE
                     VTEKVVRYSPEAIIIVVSNPLDIMTYCAYRASLKDEQKVFGMAGILDIGRYNAFIAEA
                     LKISPKDIHSLLLGGHGDTMVPLPRYTSIGGIPVTEMLNREEIDKIVERTRKGGGELV
                     SLMGTSAWYAPGAAAAQMVEAIVDDQQQIFPVCAKLNGEYGLRDVFLGVPVKLGANGI
                     EEIIELKLNEEEKKAFGESAASVKAMMNLLDEMKIFE"
     gene            17542..18234
                     /locus_tag="PKI35_05420"
     CDS             17542..18234
                     /locus_tag="PKI35_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNX43859.1"
                     /translation="MKTNIVVLTGAGISAESGIRTFRDSDGLWEEYRIEDVATYDAWV
                     RNQALVIDFYNQRRKQLYQVEPNAAHYALAKLEQKYDVQIITQNIDDLHERGGSTNII
                     HLHGELKKVRSTVDPDLIYTLDGWELKAGDKCEKGSQLRPHVVWFGEAVPLIEDAARA
                     SSHADIFIVIGTSLAVYPAAGLLSYVPDESPKYLVDPQASPLPWVKNLKIIREKAGIA
                     VPALVDELLGTA"
     gene            18331..18789
                     /locus_tag="PKI35_05425"
     CDS             18331..18789
                     /locus_tag="PKI35_05425"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNX43860.1"
                     /translation="MKRLLLLLPVAALMCLTSCFKEEDDYAAKDKKTIEKYIANHGLT
                     AQSTASGVYYVIEKPGDATHPTLSSTVTVKYTGYLTDGTEFDSSDGKAVSFALSGVIS
                     GWQIGIPLFGKGGKGTLLIPSDLGYGSYYIGNIPPHSVLIFDVELVSFTN"
     gene            18795..19298
                     /locus_tag="PKI35_05430"
     CDS             18795..19298
                     /locus_tag="PKI35_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNX43861.1"
                     /translation="MLRKQTGEPPDAAQAFRKAADYCASQERCLEETRIKMKQWGIDD
                     AVIGSTLDKLTEEGFIDEQRFAAAFARGKFRMLRWGKIKISLELRLRKIPAGYVTKAL
                     NEIDPAEYSGILKLLIQKKIKSLGDVTPDTENKVMKYAIGKGFEPELVRSAIKECIRQ
                     TTGYPEH"
     gene            19328..20423
                     /gene="prfB"
                     /locus_tag="PKI35_05435"
     CDS             join(19328..19390,19392..20423)
                     /gene="prfB"
                     /locus_tag="PKI35_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118304733.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNX43862.1"
                     /translation="MITGEQIKDLTKRLEALRRFLDIDAKLEQIEEEEKVTHAPGFWD
                     DPKKAEQLLKIIQEKKSWTNAYTEAQASFDDLLVLHEFYQADEATEEEVERNYAGTLR
                     LVENLEFRNMLSNEEDKLSAILQINSGAGGTESQDWAQMLMRMYLRWAERNRYTVREL
                     DLNEGDVAGIKSVSLEIEGDHAFGYLKGENGVHRLVRLSPFDANNRRHTSFASVYVYP
                     VVDENIDIQINPGDITWDTFRSSGPGGQNVNKVETAVRLRHEPTGIIVECQVTRSQLQ
                     NREKAMQMLKSQLYEIELRKKREAQAVIEGNKKKIEWGSQIRSYVLHPYKMVKDLRTD
                     YETSNASEVLDGDLNDFIKAYLMEYGREGE"
     gene            20675..21064
                     /gene="arsC"
                     /locus_tag="PKI35_05440"
     CDS             20675..21064
                     /gene="arsC"
                     /locus_tag="PKI35_05440"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00014.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNX43863.1"
                     /translation="MNHPNMIAIYHNPRCAKSRAGLEYLKNQGVDFEVIEYLKHPFTR
                     ESLKDLLMKLNLGPRDILRTGEAVYKEKFRGKNFTDEEWITILLENPNLIRRPIVVNG
                     YKAVVAQPPNEIDRLLSDHAINQYCHS"
     gene            21082..22473
                     /gene="fumC"
                     /locus_tag="PKI35_05445"
     CDS             21082..22473
                     /gene="fumC"
                     /locus_tag="PKI35_05445"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNX43864.1"
                     /translation="MKTRIEKDTMGPVEVPADKYWGAQTERSRNNFKVGPEGSMPREI
                     IRAFAVLKKAAAMTNMDLGVLAPEKCELISRVCDEICEGRLDGEFPLVIWQTGSGTQS
                     NMNVNEVISNRAHVMAGGILGEGNSPLHPNDDVNKSQSSNDTFPTAMSIAAYKMLVET
                     TLPGVELLRNTLAAKAEEFDKYVKIGRTHLMDATPLTLGQEFSGYVSQLDHGIRAIKN
                     SLPHLAELALGGTAVGTGINAPKGYAELVARKIALLTGLPFVTAENKFEALSSHDNIV
                     EASGALKVLATSLMHISNNIRMLASGPRCGIGELHLPENEPGSSIMPGKVNPTQNESL
                     TMVCAQVIGCDAAITVANTHGHFQLNVFKPVIISNFLMAARLLGDACASFSEHCIAGT
                     EAIERNIENHLENSLMLVTALNTHIGYENSAKIAKKAHTEGTTLREAALALGLVTDEQ
                     FTEWVDPKKMLGR"
     gene            22648..23556
                     /locus_tag="PKI35_05450"
     CDS             22648..23556
                     /locus_tag="PKI35_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNX43865.1"
                     /translation="MIQTIKIGTLRWHYIQDPSDEDLGFLKNNFHFHPLDIEDCKSVN
                     QRPKIDIYDDYYFLILHFPHYDRWNRFLKTKEVKIFWGKDYIITIGKSHWVVSSLFNS
                     ANEHSDPYEILQVATSDALLYKILEKLMQETLSLISKLGIELELINRELFSKKAEKTI
                     ERISLTRKNIIHLNTIFKPQLRLFQKFESGQIKGYAEDMEEYWGNILDYYQKMWDMIE
                     DYEELIEGLAKTFDSLQTNRTNEIMKALTIISVIILPLTLIASMYGMNIGLPLQNHPY
                     FFWMLMGFMVALSGFLILLFKKKKWL"
     gene            complement(23622..24722)
                     /locus_tag="PKI35_05455"
     CDS             complement(23622..24722)
                     /locus_tag="PKI35_05455"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNX43866.1"
                     /translation="MQNNFTIRTLEEWLPVADKPLVISGPCSAETRDQVLETARELTK
                     IPQVKVFRAGIWKPRTRPSSFEGVGNEALKWLQEVKEETGLLTAVEVAKPDHVENALK
                     HGVDILWFGARTVVNPFNVQELCEALKGLDVPVLVKNPLNPDVKLWLGAIERLYRTGI
                     SKIVAVHRGFYFYRHALYRNQPMWEVPIELQRLAPGLPIVCDPSHICGRRDLLQGVSQ
                     KALDLGMSGLMIESHFDPLHALTDANQQVTPQQLAGLISRLSVRNISGTPEFESLLEQ
                     LRQEIDKIDAELIDILARRMKIVEDIGQYKRDNNITVLQIRRWADMISERLDVGSKLG
                     LDKEFLLKLLQMVHKESIRKQEEIMGLKEEEE"
     gene            24899..26296
                     /gene="asnS"
                     /locus_tag="PKI35_05460"
     CDS             24899..26296
                     /gene="asnS"
                     /locus_tag="PKI35_05460"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNX43867.1"
                     /translation="MKRTKIEQVLKNYSQLGIGSILNIKGWVRTKRGNKNVAFIAVND
                     GSTIHSIQVVADLANFDEELMKLVTTGSCLSVTGTLVESPGSGQAVEIHAAEIEVYGT
                     ADADTYPLQKKGHTLEFLREIAHLRPRTNTFGAVFRIRHHVSFAIHKYFNDKGFCYLH
                     TPVITGSDAEGAGAMFHVTTLDLGNIPKTEDGNVDYTEDFFGKSTNLTVSGQLEGELG
                     AMALSEIYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYDINDNMDLAEDFVKYLIRYV
                     LDHCFSDLEFLQKMYDNELIQRLHSVTENEFERLTYTRAIDILLASKHKFEFPVSWGI
                     DLQAEHERYLVEQHFKKPVILTDYPKEIKAFYMKQNDDGKTVRAMDVLFPRIGEIIGG
                     SQREENYEKLRQRIVELGIPDKDMWWYLETRKFGSAPHSGFGFGFERMILFLTGMSNI
                     RDVIPFPRTPQNAEF"
     gene            26408..27886
                     /gene="rpoN"
                     /locus_tag="PKI35_05465"
     CDS             26408..27886
                     /gene="rpoN"
                     /locus_tag="PKI35_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNX43868.1"
                     /translation="MLNQRLQQRMLQKLSPQQILLMKLLQIPSLALEQRIKQEIEENP
                     VLEISDEFGDETPPDTDNEPDDVPAGETATDDEGPDNDINLADYLDDDEIPAYKLNAG
                     NQSPDDERREIPFAVSSSFQESLIAQLGLHSLTREDNIIAENIIGNLDDAGYLQRDLN
                     AMVDDLAFTQNISTNRKEIERILQIVQELDPPGIAARNLQECLLLQLRRLGTVPGIKL
                     AMLIIERYFDDFIRKHYDKIMKRSHCSEQDLREAVNIILSLNPKPGNAISEQGRDNQY
                     IVPDFIIVNKDGELELTLNQRNMPELRLNRTYVDMLEAYGENKGRKSSGSQKEAVMFI
                     KQKIDSAKWFIDAIKQRQHTLYVTMKAIMDYQYNYFLDGDETRLRPMILKDIAERVNL
                     DISTISRVANSKYVQTPFGTFLLKSFFSEAIQNDQGEEISTREIKKILSDCIESEEKN
                     NPLTDEELTAILKKKGYPIARRTVAKYREQLNIPVARLRRNI"
     gene            27964..28575
                     /locus_tag="PKI35_05470"
     CDS             27964..28575
                     /locus_tag="PKI35_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43869.1"
                     /translation="METRIARILSYILHPLLLPTLSFILIGTLPSIFVLGLPVKAQLW
                     LLGMVFTFTFLIPVTGTFMLVQFDRVRSIELKESRERTLPLMITGISYMFLYYLVKDA
                     SIPAIYLFFIYGSIIVMVAGMLINLFWKISLHTLAWGAATGALTGISLRFMLDIPLTI
                     ACTIILSGLAGFARLKLNAHNPTQVYVGFVVGAAMMLLLILLF"
     gene            complement(28567..29283)
                     /locus_tag="PKI35_05475"
     CDS             complement(28567..29283)
                     /locus_tag="PKI35_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX43870.1"
                     /translation="MKRIAVVVFLSLFSVAAFSQKPSVESITKRFRLSWEPFNDIWMD
                     VPDSINPRTINQGVNIYGLYMFPMDRKDHISFFVGAGIGAHNFFHNALIGLNDGGITY
                     LYNLPDTLGGTTIDVKKSKISVTYFDVPFGFKYKMKNDMHLTLGFKVGWKINDHWKYK
                     GDCYEADNVLYATGATIKRKYSRVPNIDNFHYGPYISFGYKWFGIMANYQISTLFEKD
                     YGPEVYPISVGLTFMPFGSK"
     gene            29476..29552
                     /locus_tag="PKI35_05480"
     tRNA            29476..29552
                     /locus_tag="PKI35_05480"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29510..29512,aa:Ile,seq:cat)
     gene            29703..30128
                     /locus_tag="PKI35_05485"
     CDS             29703..30128
                     /locus_tag="PKI35_05485"
                     /inference="COORDINATES: protein motif:HMM:NF036423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HNX43871.1"
                     /translation="MNTYHSQVKLRTDSQRKDGTCSLHLYANINGKRMFFSLHKSIPE
                     KYWNPDKQEVKIVCPNAVIINSTIKDYLQKAEKLMLSANINSKIVSKSDLEDILRSEP
                     YDSNDFFAFAENDIKEFGSKFAPATIANNNVQLKKLKEF"
     gene            30147..30338
                     /locus_tag="PKI35_05490"
                     /pseudo
     CDS             30147..30338
                     /locus_tag="PKI35_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024502.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain-containing
                     protein"
     gene            30579..31886
                     /locus_tag="PKI35_05495"
     CDS             30579..31886
                     /locus_tag="PKI35_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNX43872.1"
                     /translation="MLERPPLASESDLLESMKTAYIPEIQKLDSEYAYWDKVKYMTAP
                     DNLTSKQLWFAIKFLRQFNRTVLKFGKYEFSFTVTDEMFELLHYFDMNIGGNLTGEQI
                     IPSESKNAYLISSIMEEAIASSQMEGASTTRKVAKDMLRKSEKPRNKGQQMILNNYIT
                     INYIKGHSKDNFSVDRLREIQRSMTINTLDNESDVGNIRTHNNIMVVDGITGDIAHNP
                     PSHEELDKLLNDLELFFNKNMGSFIHPIIKAITVHFMLSYFHPFVDGNGRTARSLFYW
                     YMMKQGYWLIEFMSVSRIIYKTKVMYEKAFLYTEYDDNDLTYFLLYNLRTMKKAFEEL
                     KLYLKRKTEESSSVLMLSHIKGINQRQAQILKIVQEKPNSFFSVKEIEVRFSVSNFTA
                     RTDLDGLVKLGYLSEVKINKVKRNYIKSDRFDALIKENNVRKR"
     gene            32221..32871
                     /locus_tag="PKI35_05500"
     CDS             32221..32871
                     /locus_tag="PKI35_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNX43873.1"
                     /translation="MNTIDTIIFDLGGVLYDIDVDHTARAFTEMGAGEINMEALHKSM
                     HEEHIYEGLDTGRLSPAGFRDEIRRLTGMPLSDEQVDAAWNALLVEFPARRVKMMLSL
                     RKNYRLLLLSNTNAIHFDYYSKHFETDYGIPFESLFDKAYYSFRIGLCKPDPGIYRHV
                     ITDSKLEPEKAVFIDDLFVNVEAARKTGLKAIHLNGIPDVTDLFRDNRLDTRFLKS"
     gene            complement(32894..34249)
                     /locus_tag="PKI35_05505"
     CDS             complement(32894..34249)
                     /locus_tag="PKI35_05505"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX43874.1"
                     /translation="MEPFRIYIVEDEPLYARILEYHLSQNPDYEVHVYSTAHDLLLNL
                     YKRPSVISLDFNLPDMSGFEVLKKIREFDPELPVVIVSGQQDISTAVELLKKGVYDYV
                     LKDADTKDHLWSVMRNIKENFNLKQKITRLEEEIGRKYSYSNLIKGNSPAINKVFQML
                     EKASKANITVSIYGETGTGKELVAKSIHYNSKRHNKPFVPVNVTAIPHDLIESEMFGH
                     EKGAFTGAHAQRIGKFEEANHGTIFLDEIGDMDLNMQAKLLRVLQEEEVTRVGSNKPV
                     KLDVRVIVATHKNLLEEVKKGTFREDLYYRLLGLPIEIPPLRERDNDIFIIARHFIDE
                     FCDKNGIQRLTISADAMEKMKKYPFPGNVRELKAIVELAAIMTNSDTIHAEDIRINEI
                     EKPFDFISGEATLEEYDRRIIMHYLKKYDNKVRIVAEKLDIGKTTIYRMMKNDAGELQ
                     E"
     gene            complement(34354..35346)
                     /gene="dusB"
                     /locus_tag="PKI35_05510"
     CDS             complement(34354..35346)
                     /gene="dusB"
                     /locus_tag="PKI35_05510"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNX43875.1"
                     /translation="MKIGSLDLGEFPVLLAPLEDITDSAFRVICREFGAAMVYTEFIS
                     SEGLVRDARKSNDKLLFEECERPIGIQIFGSDPEAMRLAALRAAEAKPEVIDINFGCP
                     VKKVAMKGAGAGLLNDVSRMVKITGEVVKATSIPVTVKTRLGWDDSHKNIVEIAERLQ
                     DEGIAAITIHGRTRAQLYSGTADWTLIGEVKNNPRMSIPVIGNGDIDKAEKVFLMKEK
                     YGVDAVMIGRAAIGNPWIFKEISGFRSNRSAVMPATGIGERVSVCLRHLHKAVENKGE
                     HRAVVEMRRHYAGYFRGMYDFKKYRIQLLTLKSESEIIGLLSEIKQNYPDSGPE"
     gene            complement(35519..>35733)
                     /locus_tag="PKI35_05515"
     CDS             complement(35519..>35733)
                     /locus_tag="PKI35_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43876.1"
                     /translation="QAGLPPDAQPSQAGRSETLKLCPRITNNETQTVPSKLRTQNAKD
                     QEPMAKSQKEKVKRSKGQRSKGKGKR"
BASE COUNT         9745 a         8571 c         8152 g         9265 t
ORIGIN      
        1 ggcctgccac ctgacgcaca gccttcccag gcaggcaggt ctgaaacact gaaacactaa
       61 aacctgcaac caacagccaa tagccaatag ctaaaagcta aaagtttacc tgcctggacg
      121 aggttgtggg taagatgaca gggcaaggag taaggagcga ggagcaggga gaacggagga
      181 cggaggacgg aaaatggaaa gcggagggcg gggagcggaa ctaaacacaa aacacaaaac
      241 gcagaatgca gaactctgga acactgaaaa actgaaacac tgaaactttg aaaccctgcc
      301 ccctcctaca gcccagcgca aggcaggcag gcctgccacc tgacgcacag ccttcccagg
      361 caggcaggtc tgaaacactg aaactctgat actctgaaac cctgaaacac tgaaacacta
      421 aaaacctgca accaacagcc aatagccaat agctaacggc taaaagttta cctgcctgga
      481 cgaggttgtg ggtaagatgg caggccaacg gccaaaagcc agaaattgat catcttctgt
      541 ccgctgcccg caggcagtaa cgatgcagac cgctttaaca aaattctgat attaatatgc
      601 ctcctaaaac agtccatctc gaaaatatcg tcagggacct gcccgacagg ccgggggtgt
      661 accaatatta cgacaaggac ggtaaattgc tttacgtggg caaagccaag aacctgaaaa
      721 agagggtttc atcctatttt acacgcgaca gttccctgac gggaaaagta aaaattcttg
      781 tccagaaaat tgctgatatc cggtttatcg tcgtcgatac agaactggat gccctgttgc
      841 tcgagaacaa cctgatcaag caataccagc cccgctacaa cgtaatgctg aaagatgaca
      901 agacctatcc ctggatttgc atcaaaaatg aaccattccc ccgtgttttg cccgttcgca
      961 acctggtaaa agacggctcg gaatattttg gcccatatgc gtcggtgcgg atgatgaata
     1021 cactgctcga cctcatccgg cagctttacc ctctccgaac ctgtacgctc aacctttcag
     1081 aatcgaatat ccgccagaat aaatacaagg tatgcctcga atttcatctg ggcaattgca
     1141 aagggccctg cgaagggctc cagctacagg atgagtacct ggaaaccctg aacgggatca
     1201 gacaaatcat caggggaaac attgcttccg tcaggcaggg actgattata ctgatgaagc
     1261 aggcagcagc agggtatgaa tttgaaaaag cgcaattcct gaaagagaag atagaactgc
     1321 tggatcgtta ccaaagtaaa tctgccgtcg ttaatccctc catacataat gtggacgtct
     1381 tctcctattg tgacgattta agtgccgcct atgtcaattt catgaaagtg ataaacggtt
     1441 gcatcatcca aagtcacacc atcgaactga aaaagaagct ggatgaatcg ccggccgaat
     1501 tgctttccct tgccattacc gaattgcgcg aacgctttca gagcgacgcc cgtgaaatca
     1561 tcataccgtt tgcccctgat gttgaattac aggatattac aactacgatc ccgctcaggg
     1621 gcgataaaaa agccctactc gaactgagtg aacgcaacgc gaaatattac aggcttgaga
     1681 aaaacaagca gctcgaacgg gtcgatcccg agcggcacac aaaacgcatc atggaacaga
     1741 tgcaatccga tttaaggttg aaagagcaac cgctccggat tgaatgcttt gataattcga
     1801 acatccaggg cgcatacccc gttgcctcca tggtggtttt caccaacggc aaacccgaca
     1861 agcaggaata ccgccatttt aacattaaaa ccgtagaagg tcccgacgat tatgccagca
     1921 tggaagaggt gatctatcgc cgttacaaac gcgtgctgga agagggaaaa ccgctgccgc
     1981 aacttatcgt gatcgatggc ggtaaagggc aactgggatc tgcgatgaca agcctcggga
     2041 aattagggtt gagaggaaaa atagaagtca tcggcattgc caagaaactg gaagaaattt
     2101 ttaagcccgg cgacccgatt ccactttaca tcgacaaaaa atcggaaacg cttcgcatta
     2161 tacagcagat acgcgacgaa gcgcaccgct tcggcataac acatcaccgc aaacgccgcg
     2221 aaaaagggac catcaaaacc cagcttaccg aaatcgaagg catcggtatg gcaacggcac
     2281 aatcgctgtt gaggaaattc aggagcgtaa agaacatcgg taacgcttcg ctggaagaat
     2341 tgcaggctgc cgtcggcaag gctaaagctt cagtagttta tgaatacttc cgatcgcaaa
     2401 atcaatgaaa gcgatcgctc aacctctgag atgaaataag gcaggtaact tatagtccct
     2461 gccttatttg tacaccgttt gttttacccg ggtctattct cctttacagc caaaatgaat
     2521 atcagcgcca atggcgagga tcatgttgtc cttgtcatag gtttccacaa ccggaacagc
     2581 cgtcgatcct aacatatgcg tcatattttt atcagcatcc ttaaaaaccg aatacatagc
     2641 cccaacgtta agctggaaat caggggtaat attaacagcc agtccgcagg cgcctgaacg
     2701 ggaagtaagg ctgttgctga gatccgtctg gtaggtttct tttacgcccg tctgggccag
     2761 caggtatcct gcgctcacca gccaccgttt cccgatttta tattcgaggc ccagcgcata
     2821 ttccctgaag ttatgatcaa tcaggtccgt attatccaca tattccccgc tcagtttctt
     2881 tccgtaatcg gcagcctcgt cccaataggt atggaaaccg gccgaaacca aaaacttgtc
     2941 ggaaacatta taggcagctc ctatcgacat cattgcgggc atatcgttat aaaacgattc
     3001 accgtcaggg aacatggtga tttcggaacc gtcgggtgca aatccgccaa ggacatcctt
     3061 cttggtatta tttgttacct tcatatggac gggcatttca tatttaaaac ctatattaag
     3121 cttctgatca agaaggttaa cattcaggcc aaaaatcggg gttattgccg agcctttctg
     3181 ggtaacatca gcttcctggt ccgacaacag ggttttcgtt gcctgggctt tggctgcgcc
     3241 gacattgcag gtttgcacag tctggttcat gatggcaacc gcctgtccga aagttgttcc
     3301 ggccggaagg ccggctgaag ctgcaacatc attgggcatg acaagatcgg caggatattg
     3361 tgaaaattgt ccgattgccg ccgtagcttg ttgtgtgcct gtcaggtact tctgtctgat
     3421 gatttcgata aaatcaacag caggaatttc tgttgaaccc atgatcagcg tattgtttcg
     3481 caggtatcct tcataggttg tgttggcggc aataacacgg aaaccgccaa atattgaaac
     3541 atttttattc acttcggcgc tgacgcctaa ctgggcgccc caataaacag atctgccctt
     3601 gaaatagatg tcggttttat accctgttac tccgctcgat tgaaggctgg gcacaaggtc
     3661 agagatgctg tattcaaacg aaggcaatcc tttgtcaaac acagccccgc caccgccgcc
     3721 tatcggattg aaaccgaagg atactgcaaa gcggtcaaat ttataagcag catataccga
     3781 cggaaacaca ggcgctacga tcttacctat atattctttg ttattcagat tcaggtaatc
     3841 acttttaata ttccggtcct gccagatgct ctggttcgat accgacagat gaaatccctt
     3901 ttttaacaac atgagtccgg cgggattaaa ataaacggca tcaattcctg ttgaggcgtc
     3961 acgacaaagc atccgcacgt aggctgcgct ttgattggca ttggtaacga tgccgccggc
     4021 gcgtgaagct gcagcgagca tcagcaacgc caggataaca agtacagttc ttttcatggt
     4081 tttgtttttg gttaatgata gagagtctgg tttttcacga ataagtgcga taaaataaac
     4141 actaaatatg cgaacttcaa aatatatttt ttgattttat aataattaat atttcatata
     4201 caaaccagta tcctgagcta tcgggctttt acggaagaat ttgtgcacca taaaaatgta
     4261 acgacgggca gcatttcagc caaaacaaat catcctgaaa taaaaaaccc gctcaaaaat
     4321 gagcgggccc ggaataaatt ccactgtttc ttagtttgcc aatgtaaacc ggataggcag
     4381 gttaaactgt acacgaaccg gcttgccacg ctgcttgccg gggttccact tgggcatagc
     4441 ttttataaca cggatggctt cttcgtcgca accaccgcca attccacgca gcactttgac
     4501 atcggtaaca cttccatcgc gttcaaccac aaaagtaaca aaaacggttc ctgatattcc
     4561 tgattcacgg gccatctgag gatatttcat gttttctctg aggaaattga tacgggcttc
     4621 atctccgcca ggataggacg gcgattcttc aacaacggta aaaatttcag cttcggcaac
     4681 ttcttcttca accacctggg tagtaacttt tacaggagcc gtatactgct gcatctgggt
     4741 attctcatcg gcttcggcgt caatcttgaa atcgtcatca acaatggcat cattatttac
     4801 aatattgatt accgtagttg ttgcaggagg agcaggaggc gggggggtat tctgctgttg
     4861 cgttatctgt accatttctt cgggcacatc tacgaagttt ttggtgtcat aaccttccac
     4921 cactgccttg tcatagcttt tatattcgaa agctgcaagg gtaatggcaa gggctacgat
     4981 gagcccgatc tgtaaaaaga cagtcttttt actttccaga tcagccttag cggatttttt
     5041 cagttccatg gtaattgtta ctttgttatt tatactccac gtactctttt acaaatacaa
     5101 aattacaaac aattttaact ttccttccca tcccgggcaa tattttatgc cgtttataat
     5161 ttgccatcag cttcctgttg aactgtataa acaacagaga gaggaaagac gctattaaaa
     5221 cagatgcaat tgcagccacg tgtaccaggt agtgcctgct acacttacat taaagccttt
     5281 tcgatcaggc ttctataagt ttctgataat cggcaggttt cagcaggtca ccaagctggg
     5341 aagcattgtc gatgcggatt tttacaatcc agccttctcc gtaaggatca ctgtttacta
     5401 cttccggttt gtttacgagg gcttcgttga attcaagaat ctcgccgtca acaggaatga
     5461 gcatatccga aacagtcttt actgcttcaa tggtaccaaa agtttcgccc atggccactt
     5521 tttcccccac ggtatcaact tcaatataaa caatatcgcc aagttcatgc tgtgcgaaat
     5581 cagtaacgcc aacaatggct tcattgccgt ttaccagaat ccattcgtga tcgtttgtgt
     5641 acaggagatt ttcaggaata ttcatcttca gggttgttta attattcgct tacaaatgta
     5701 gaaatttgac agaatacagt agccgcggac tgttaaatta gaactattct gaataatgtc
     5761 tttttcaggt tgagaacatc ataacgcccc ggggttactg ggtaagggta aaccgcaagg
     5821 aaatgccggc attggttgtt gaggtgggaa tctgcgacag gacgtgtggc ttggttatgg
     5881 tggactcata gtacaacctg aatgtcagtt tctggcttac catataatcg gcagaagcat
     5941 ttatagatac ctgcatggag cctgttgaaa tctggttgtt gttttcttca atcctcctga
     6001 gaacggtctt gttatccctc accgatacat cgagtttcac gttcaggtcg cttttaagtt
     6061 gccgtttagg cccgccgctc accgagcgga cattaaaccg tatgttcttg aaacgatagc
     6121 caaccccagt gaccaactcg ttgctcctga cttcggtaag ctgattattg acaaagctca
     6181 gcgacaggtt tctcgtcttt ttatactcga ttcgcgtcat caggctgttc ttcatagtca
     6241 tctccacccc gagaaacgga ccgaattgtt cggtcagggt aaccacttcc atgtattttt
     6301 gaggaataaa attgcccagg tcgtccatag ctgaagcaaa tccaggcaga ttttcatttt
     6361 cctgataggc gacattggtg ttatatccgc cgatgctgta tgccgaacga taggaatggg
     6421 tgatgttgaa ggactggaaa tgatcacgta tggcttttac ccctgaatgc aggttatagg
     6481 tcaaccgcca gtttggtaac ggaatggcgg ggaatgcttt cagcgatgct gaagaaggat
     6541 tcctgcctga ataggtagtg aggaaggagt acagcaagac ctcggcctgg gttgcaccat
     6601 agccttgcgg gaaaccggtt gtatcgttta tgcctgctga atttggattt ttttcggcaa
     6661 ggcgtccggc aataatattc ctgttttcaa gcatttgctg gaatgtggcg ttttcattcg
     6721 ccttattgtc acgaatgaag gctgtgggcc agatgatata agaactgctg aaattgcctc
     6781 tttcctgcgc cgatgtcatg tcaaatttac catcacttcc ggctttccag tatccctgat
     6841 acgtcgaaga ataactcctg tcggctgtca gttctatttt catattgcgg aatggctcca
     6901 ggttagcccg tccggtaagt gagctggtag ccctgtcgat gtaagggtta ttgagcagcg
     6961 tatcggtact cagccatccg cgcgattcag ccaccatccg tatatcctgc tggctgccaa
     7021 aaatgaagcc taatccaggt gcatcgtcct tccagttatt gcccaacggg cctgaaacag
     7081 gtttaaagcc cggaagcgtc atcccgttgg tctgattgta ggtaacagaa gcatccttaa
     7141 aagccataag cacgcccaag gcctggttcc cgatgattcg caggtagtcc acctttggct
     7201 tgttttcggt cgtatcggca tcactggctt tctccgcctg cttcttcaca tcttcctttt
     7261 cccggccttc ccgttccatg cctttgcggg ccggaggcgc aggtttctgc ggagagccca
     7321 gcttttggtt ataatccagg gcttttttca ggaaaggcac tttattatac aaggaagtca
     7381 ttctggcatt accgttaagc tgcaggttac gcgaattctc aatggtattg ccgtaccggg
     7441 cctgcagcga ttgaggcgaa gcatcccagc gatattgagc gccgtaacgc gccgagcccg
     7501 tcaaccagtt cagcagggat attttgctga agggtatcat atagttgaga ttggcagttt
     7561 ggttgaaggt attcatcgat ccgaaaccca ggattgactg catcaccgaa tcgcggtacg
     7621 cctgatatga gtcgtcacgc ctgttaacac cgccggcagg ttcatcaaca taagcctggg
     7681 cgtttgcgtt gtaatcaaat ttcagggatt gggaaaaatc atatttgaaa tcgtacgacc
     7741 ggctccagtc ccagtttttc gtataggtag gttccaggat gatcagggct tcgctcttat
     7801 tgcgcaacag cctttcctca taagtgcggt acatctccga acggatcgac aacaacttcg
     7861 gcatatagta aaaattgaat tcactgagca ttttcaatga cttgcttttt actattttct
     7921 ggaacggcct tacattcttg ggattggccg aaaaattgta gccaatgctc ccccgatagg
     7981 actttttgat gtctttttca atatcggcat tatgcgtgtt gatttccgaa taagcataag
     8041 agagatcgaa attttcaata tcccacaagt gcatcttttt ggtattgccc accctatcct
     8101 tcctgacatt catcaggttc aggttctggc gctgcactac gtcggatgtg atggcacgta
     8161 acgaatcccg gtcttctttg ctgagcgaat tcaattgttc gttcagctta atatccggat
     8221 cgtatggatt atactgcggg ttggttactg actttgaata atcgtagtga aagggaatcc
     8281 tgatacccga tttttcaggc aggaattttc ccagttccag attcgtggca aagtcgatct
     8341 gtctgatgct ttcctgctgg cgctccgaaa ctttctgatc aatgcttccg aacccgggag
     8401 tactggcaga accgctgaca actacatttc ccaggtcggc aagattggcg ctgatacgtg
     8461 cattagcggc ccagcccgat tgttcgttaa agtcggtaag ccttaattca ttcacccaga
     8521 tttcgacatt tttcgactgc ccgtcatcgg tattgtcaaa ggaactgtca cgtttcgggt
     8581 tccgtacccc gatcatgaag gcgcggacat cactgaggct gggtgatccc acaatcgtga
     8641 tattgttatt cccgtctgta atggtgaagg gtaacgatac gcttacggcc gaaccggaat
     8701 tttccatggc ggcattgcgc ctttttttga cttcaaccag cttatcgagt tcgatgttga
     8761 attcattgga caacggccat accgaacgtt gtccttcttc tgtatcggta tatcttcccc
     8821 agggagtata ttcaacggga acttcatatt cgtagtagtt atttgtaaaa tcagacccga
     8881 tgcggataaa caccgtaagg tcgccattta catggctttc agcttcagcg gtcttttcag
     8941 catggacaaa catcttcagt cgtttgtatt ccctgaagtc gaaatcggtg gttttataag
     9001 ccccgcgggc atcgccatcc atcaggttgc caactttcaa caccatagcc tgttcgttaa
     9061 gctcatagta gttggtcgac tgggcgttga tttcgcgttt gatgccaggc ggtgttaaat
     9121 agttaatatt ggccctgtta taattttcct caacattcac cgatgaaata tcaaaagcgg
     9181 tagcatcctg gttgggatcg gggatatatt cgcccggcga caacaggctg ttgtaatatt
     9241 tgcgccattc gccacgttcc agttccaggg aaccaaacct gcaaactaca ggctgctcga
     9301 aattgcgcag gaacatcctc atgaaacgga tcgatttgaa atcctggatg cttcctacaa
     9361 ccttgtcagg actttggata ggaattttga actgatacca gtttactttc ccggaatgtt
     9421 tatcgtcaat ctggtacgat gcttccagtt tatcggttat gtaattctcc ccgacattca
     9481 gccccgaagg ggtcagatgg ataacatact gaaaataacg ttcctggtcg ctcagggtgt
     9541 tatcgctgtt aatgtcctcc atgttcggcg tcgtcgtggc aagggtttga tatgattcag
     9601 ggttcattgc aggcgttggc gagttgccgt cgggattgtt gtaattttta taccgttcga
     9661 ccaggctgct gtatttgtcc tcgttatcgt aatcgcttcc cctgaaatag tgatagttat
     9721 cggatgaagg gtctttgtct gcatcctggt aagcctggga agtgattccc cattttgcag
     9781 cgattttgtt aagaagtaca gaactgaaaa attgcttttc gtcggaggtg gacaggccgt
     9841 cataccccac atcctgataa ggacgcgcgg catcgtccga aacgaaggaa gcggtaattg
     9901 cctgcatggt cggtaccaat ccccagatgg tggtatccac attttcctta acttcactgg
     9961 taggaaggcc atgttcaaac gattttcgac catcgcggag gatatcttcc gaaacatccc
    10021 ccaggttaaa gtaaaggtct ccgcccttgt ttgacgggtc gtcggcaaac gggtccatca
    10081 gccagaaagt gatgtattcg atattagcag cgtcaaagtc ggaggtttcg acacggcgca
    10141 tgattccgcc ccagcgcgaa gcaggatttt ttaaggtccc gtcttcgttg ataccgctcg
    10201 aaaacgtcga tgcctcgaca tcataattgt aaggtccacg ctcttcagga aaatacgcta
    10261 tattaaatac ctgaatattc gtgggctgac ctgtctccga ttccttcctg ggaaacaatt
    10321 cgttttgcaa caccacgcgg actgaatttt tgctgatttc ctccttggtg acgttctttg
    10381 gacggagatt gctggaatta tcataaaaca acgggtcgat ggtataccag gcaattttgg
    10441 cgcggttaaa gccgtaagcg aggcctgtac cgggagccgc ttcaggaaac agggtgcgga
    10501 ttgtttgtcc ctgcggggtg ctggcaagaa accaggcgcc gacattccgc aagtcaatcg
    10561 tggatttcgc cccttcaaaa tcgtcaatat aagaagtacc tgtttttcca acagctttgg
    10621 aatgccccgg caggaactgc gcaaattccg catcgatacg gagtttgctg gctgcattgg
    10681 tactcaggaa aggcagttta tctacaattt ttgttatcag ccccgactct ttagaataat
    10741 ttacacttcc cccccaaatg gtattggaga tcggttcgtc accgtagttt gtcttttggg
    10801 tgatcggccg ttcatagagg ttaagcaggg tgcctcccaa taaaaggttc ttgtttacct
    10861 tataatcgag atgggtcccc atcagcgttt gactgtaaac attgaacatc gagttgtttt
    10921 ccagcgatat attgatcggg gtgccggaat tcaggatacc ctggttaatg attttcacac
    10981 ggccaagcgt ataatcaacg gtataatcaa cattttcaac cagcgggatt ccgcctgccg
    11041 tcaccgttac cgatccctgg ggtacattca gcgcgttcag ggatatatcg gaacccgatt
    11101 cggacttata ctgcccttca agtatatact tgttcttatc cgggaattgc tgggccccgg
    11161 tttttgtcat ggtatagagt gaatcaaaac aaaccttgtc ggcaatggtc ttatcgttga
    11221 taagatttcg caggtaactt ccgaacggct ccacgaccgg gaagaaaacc cggccgttcg
    11281 acgactggat ggtacctccg ctggtagctg cattgccgat aaagtcaaaa attccgtcag
    11341 ccggcggatt tgattgagga tccaaccggt ccaggcccag aagctggatc aacggcacgc
    11401 ccttgatatt atccggtccc gatgtaaaat accctgtcgg aacagcattg ctgtttcccg
    11461 agtaaagaac gttcagaata aaatcctcgc tgtttacatt ataagcccca aggttgtaaa
    11521 catttttcat catgaggttc cacatgggaa tatgtgtgtt tacagcggta cttttcagca
    11581 attttaccat caggttcttg gtggtcgcaa taccctgatc ggaaaattcc ccgacctggt
    11641 aaacgctgtc gctgccaatc accgtatatt gaaatgcaac ggccagggtt tggtcagggt
    11701 tgagcgatga attcagcgag ataaatccca gtttactgtt aaaggtatat tcggtggagt
    11761 tcagcttgcg ggcgctctca accttttcaa aatcaacgcc gggaacatac cctttgaccc
    11821 ccgtcaggta ttcgtttacc ttgttgatat tcctgaccct ggcagtatcg agattattga
    11881 gcagatcgtt gttgtaattc gacggatagg gattcaggga caaggggtta atatgactgt
    11941 tgtaaggatc ggattcgccg aggtcctgga aagcgacaat gttgcggttt tcggtaacgg
    12001 cagcgcctat gttggtgatc cagacttcta tcttggtaat gttgatgttg gaggttatga
    12061 ccgggagcgt tttaagcgct tcatcgtaat gattcctaaa atactgcccg ataaaaaagt
    12121 gcttgttctc ttcatattcg agcgacctca ggctaaaatc ggttgtttgc gccccgccct
    12181 gtacgcttat attttcaacc tggctctgct gctgggagaa aactgtggta aaggtagcct
    12241 tcccgaattt taactgggtt ttgagcccga acaggctctg gctcccggtt atcagggaac
    12301 tgttcagcgg gaggttgaca ttaccggctt ctatcaactg gataatttcg tcttccttgc
    12361 cttcatattt caatttgagc ttgttctcaa aatcgaaagt cgcttcggta ttgtaattgg
    12421 tattgaattc aattttatcg ccgatcttgg cgagcacgct catctgaatg cgttcctgga
    12481 aatcgaaatt ggtcgtacgg cgcaaacgcg tgtcaatggc aggatcgtcc cgacggttcg
    12541 atacgatccc gaaaaccagt tcggcgcttc cctgcggcct gatgtcgatg gtgttgccgc
    12601 cgaaaacacg gtcgaaaagt tcaccgccta tccgcaactg cggaattatt ccctgcctgc
    12661 ccccgacgcc accggcgctt atcttctcct gccagtaacg ttctaccgac tgttttaaat
    12721 cccagtcttt gtattcatca aatgaaaacg atgctggacg cgtgtaatta aggttaccga
    12781 tcttgcgggt gaggacatat tcgttgagaa tggggtcata gataatttct tccttcaggt
    12841 ttgaagggtc tttcaggtat aatccgccgt tttgaagttg atcataagga ttgatgctct
    12901 gccggatggg aaagcggagt gttgtatcat taccttcagt tgaatcggga gggttcacaa
    12961 aatgacctcc ggattgttcc ccgggcgcat ttccccggcc gggacttgta ttccaggaaa
    13021 caaggattac gaaagcaagt aatgcaagcc tgagtatata tttaaccaga ttcttcaaac
    13081 gataaacttt attattgctg gtgtttgttg tgttttttta acctgacaat acgtaggggg
    13141 taaccgtttt aaccattgcc ctgtcaaaaa caaaagcgag cctgtttctg gcttataata
    13201 tttttaacgc atttttaatc atttgttcca caggcaacga aaggccatcc tgcttcacga
    13261 cggcttcaag ggccttatcg gcagcagctt tgttgaaccc caacattacc aatgctgata
    13321 acgcttctac ctttgctgta ttgtgttttg cgccgaatat ttcttttgtt acactttccc
    13381 tgctgagcgg tcctttcagg tcgacaataa ggcgaagggc cgatttgctg ccaatgcctt
    13441 tcaccgactg caacgctttg acattttccg atacgatggc atccgttatt tcatcggggg
    13501 tgagagccga taaaatgagc ctggcggtat tggggccaac tcccgaaacc gagatcagca
    13561 actgaaagat actccgttcc gcttcatcgg caaagccaaa taaaaccagg gcatcttccc
    13621 gaaccaccag gtgtgtgtat actcggcaat tttctttgcc gttcagtttt gagtaagtgt
    13681 tcagcgaaat attaagctgg taaccaactc cgttgcagtt gagtacgagg cccgctggat
    13741 tcaaatcggc tattttgcct tcaataaatg catacatacc ttaaatatta acagtatcaa
    13801 acagatccgg caattttacg gcaaaaatag gattttcccg ttacatggaa aaggcagctt
    13861 agatcccagc ctcgcctgtt taccaataaa attaaatgct ctaatattgc agcgcttttc
    13921 caggcattgc cccagaaagg caaagagtta ttaagactca gtttaaattg aaaggatagc
    13981 tatgtgaaaa acaacatttt atgttaaaat catgtaatta tttggtatgt ttgttataca
    14041 gcattcatgc aaaaacaacg attgataata ccccaatgtc atgaaatcat tactgcagaa
    14101 aaatgccctg agttatcatg ctgacgggcg cccgggtaaa attgaggtca ttcccaccaa
    14161 accgcatgcc tctcagcgcg atctttcgct tgcctatacg cctggcgtag ctgaaccatg
    14221 cctcgaaatc aaagcaaaac cggacgatgt atatacatat acggcaaaag ggaacctggt
    14281 agccgtaatc tcaaacggta cggctgttct tggtctggga gatatcggcc ccgaagccgg
    14341 taaacctgtg atggaaggca aaggcctgct ttttaaagtt tatgccgaca ttgacgtgtt
    14401 tgatattgaa gtcaatgaaa aagatcctga acggatgata gcaacctgca aagccatagc
    14461 ccctactttc ggcggcataa acctcgaaga tatcaaagcg cccgaatgtt tcgaaatcga
    14521 ggaacggctt aaggcagaac ttgacattcc ggtgatgcac gacgatcagc acggaacagc
    14581 catcattacc tcggcggcct tgctgaatgc cattaccatc ataggtaaaa ggatcgaaga
    14641 ccttaaaatt gtggtgaacg gggccggagc ggcagccgtt tcatgtacca ggctatacgt
    14701 taaactgggc gcccgcaggg aaaatatcgt gatgatcgat agcaaaggcg tcctgaacaa
    14761 gaaacgcacc gaccttaacc gctacaagat ggaatttgtc accggccgcg acatcgatac
    14821 gcttgaacag gcgattgaag gggctgacat gttccttggc ctctctgttg ccggggccct
    14881 taagcccgaa tggcttctga aaatggcccc aaaccccatc gttttcgccc tggctaatcc
    14941 ggtacccgaa attgcctacg atgtagccca tgctacccgc aacgacctgg tgatggcaac
    15001 cggcagaagc gattatccca atcaggtaaa caatgtcctg gggttcccct atattttcag
    15061 gggcgctctc gacgttcgtg ccagggccat caacgacgaa atgaaacttg ctgccgcttt
    15121 tgcccttgcc gaacttgcca aacttcccgt acctgaagag gtgaacatcg cctataatac
    15181 gaccaacctc cgcttcggca aaaattatat tattcccaaa ccctgcgatc cgcgcctgat
    15241 catgtatgtt gcacaggcag tggccaaagc cgcaatggag accggggtcg cccgtaagcc
    15301 tgttacagac tggaatgcct atgaaacgga attgggaaag aggcttggcc ttgttaatcc
    15361 tctcatccgt ttcatccggg acaaagccag gaatgaacca aaacgtgttg tttttgccga
    15421 agctgaacat tataaaatcc tgaaagccgc cgaaattgtt aaagatgaag gcattggctt
    15481 ccccgttcta ttgggtgata aggacaagat cggccaaatc atcaaagaat accaacttga
    15541 actgaacggg gttccgatta tcgacccgaa atcggacgaa caaaaagaaa accggagaat
    15601 cttcgctgaa caatactatt ccaggcgtat gcggcggggc accaccctgc aggcagccct
    15661 tgaccggatg atgcaccgta actatttcgg tcccagcatg gttgaaaacg ggcttgccga
    15721 tgccatgatc tcaggactga cgaccgaata cccggaaacc atacgtccgg cattgcgcat
    15781 cattggcaaa aaaccgggag tgcgggtgat ctcgggaatg tatatcatgc ttacaaaaaa
    15841 aggtcctatc ttcttctccg atacaaccgt aaacatcaat ccgacggtgg aaaccctggt
    15901 tgaaatcact cttgaaacag cggctgccgt gcggaccttt aacattgagc cccggattgc
    15961 catcctctca tactccaatt tcggttccgt ggaaggcgat gttcctctca gggttcgtga
    16021 aacagtgagc cggctgcatc aagaatatcc ggacctgatc gtcgacgggg atatgcaggc
    16081 aaacttcgcc atgaatgaag agttgcttgc cgaacatttc ccgttcagca aacttgccga
    16141 tggaaaaggc cctgccaata ccctgatttt cccctatctc accgccggaa acattgctta
    16201 taaactcata caggaactgg gcggagccga agcgatcggg ccggtgctga acggcttgaa
    16261 caaatcggtt cacgtgctgc agatggggtc gtcggtcaat gagatcgtaa atatgacagc
    16321 catagctgtg attgacgcac aaacccacgg gcctcacggc gccatagctc atgaatgctc
    16381 cgggatgccc gcctaggcgg gcatcccggc acctgcttcc aagcccggtc aggtattggt
    16441 ttgcaccggc gttatgtttc ggatgaacaa ttatcggttt cacatgtcta tactcacatc
    16501 tttaatattt tcgtaatcag acagataaac gataaaatta aaaccgatga gaaaaataac
    16561 cgtaatagga gccggaaatg tcggggcaac tgttgccaac gttatcgctc acaaggacct
    16621 ggcaagagaa atagtattgg tggatgttaa ggaaggcatt gccgaaggca aagcgctgga
    16681 tatctggcag acttcatcca tcaataactt caacacccgg gtaataggcg tcaccaatga
    16741 ttacctgagg accaagggat cgggcattgt ggtcatcacg gccggtttcc cgcggaagcc
    16801 cggtatgagc cgcgacgatc ttatcaaaac caacgccctg attgtaaaag aagtcaccga
    16861 aaaagtcgtc aggtattcac cggaggccat tatcattgtt gtatcaaatc cgcttgatat
    16921 catgacttat tgcgcttacc gggcctcgct gaaggatgag cagaaggttt tcggcatggc
    16981 aggtattctt gatatcggac gttacaacgc tttcattgcc gaagcattga aaatatcccc
    17041 caaagatatt cactctttgc tccttggcgg ccatggtgat actatggtcc ctttgccccg
    17101 ttatacttcc atcggcggaa tcccggtcac ggaaatgctg aacagggagg agatagacaa
    17161 gattgtggag cgcacccgca aaggaggcgg agagttggtc agcctgatgg gcacctccgc
    17221 ctggtatgcc ccgggcgctg ctgctgccca aatggtggaa gccatcgtgg atgaccagca
    17281 gcaaattttc ccggtttgtg caaaactcaa cggcgagtac ggcctcaggg atgttttcct
    17341 gggcgttccc gtgaaacttg gcgccaacgg gattgaagaa atcattgaat taaagctcaa
    17401 tgaggaagag aaaaaagcct tcggggaatc ggcagcctcg gttaaagcga tgatgaacct
    17461 gctcgatgag atgaagattt tcgaataagc tatcccgtca tttattaatt tcgggctcct
    17521 tatcatcgct tctttttttc tatgaaaacc aacattgtag ttcttaccgg agccggaata
    17581 agcgccgaaa gcggcatccg cacattccgc gacagcgacg gcttatggga agaatatcgt
    17641 attgaagatg tcgccactta cgatgcctgg gtcagaaacc aggccctggt tattgacttc
    17701 tataaccagc gtcggaaaca gttataccag gttgagccaa atgccgccca ttatgccctg
    17761 gcaaaacttg aacaaaaata tgacgttcag attatcaccc agaatattga cgacctgcat
    17821 gaacgcggcg gttcaaccaa tatcatacat ctgcacggtg aattaaaaaa agtacgttcc
    17881 accgttgatc ccgacctgat ttacaccctc gacggatggg aactcaaagc gggcgacaaa
    17941 tgcgaaaaag gatcacagtt acggcctcat gtggtttggt tcggcgaagc ggttcccctg
    18001 atcgaagatg ctgcaagagc ttcttcccat gcggatattt tcatcgtcat cggaacttca
    18061 ctggctgttt atccggcggc aggcctgttg agctatgttc ccgacgaaag cccgaaatac
    18121 ctggtcgacc cacaagcttc tcccctgccg tgggtgaaaa atctgaagat tatcagggag
    18181 aaagcaggga ttgccgtacc cgccctggtg gatgaactcc ttggcacagc atagccaaat
    18241 tcactaagtt ttacttccga accggcggcc gccagcgtat tgtcaggcat atcgcacaaa
    18301 atcatacctt tgtaccgaca attcctgaac atgaaaagac tgcttctgct attgcctgtt
    18361 gccgcactga tgtgtcttac ttcctgcttt aaggaagaag atgattatgc tgcaaaagat
    18421 aaaaagacca ttgaaaaata cattgcaaat cacgggctta ctgcacaatc caccgcttcg
    18481 ggcgtatatt atgtgattga aaagcccggc gacgctacgc atcccacctt aagttctaca
    18541 gtaacggtga agtataccgg ctatctgacc gatgggacgg agttcgacag cagtgatgga
    18601 aaagccgtat cttttgcgct ttcaggggta atttcgggat ggcagatcgg catcccacta
    18661 tttgggaaag gcggaaaggg aaccttgctg ataccctccg acctgggcta tgggtcatac
    18721 tatatcggca acatcccgcc acattccgta cttattttcg acgttgaact tgttagtttt
    18781 accaattaac ggccatgctt cgaaaacaaa ccggcgaacc gcccgatgca gcccaggctt
    18841 tccggaaagc tgctgattat tgtgcttcgc aggaacgatg ccttgaggaa accaggataa
    18901 aaatgaaaca atggggaatt gatgacgcag tgatcggttc cacattagac aaactaacgg
    18961 aagaaggctt tatagacgaa cagcgttttg ctgccgcttt tgcacgcggg aagttcagga
    19021 tgctgagatg gggaaagatt aaaatttcgt tggaattacg tttgcgtaaa atccctgccg
    19081 gatatgtcac caaggcgctg aatgagattg atccggcaga atattcaggt atactaaagc
    19141 tgttgatcca aaaaaaaata aagagtctgg gtgatgttac ccctgacacc gaaaacaaag
    19201 tgatgaaata tgcaatcggg aaaggttttg aacctgagct tgtcaggtcc gctataaaag
    19261 agtgtatccg gcaaacaaca ggataccccg aacactaaat cagtattcat aaaattcatg
    19321 ctgccagatg attaccggcg aacaaattaa agatcttaca aagcggctcg aagcgctgag
    19381 gaggttcctt tgacattgat gccaaactgg aacaaatcga agaagaagaa aaagtaaccc
    19441 atgccccagg gttttgggat gatccaaaaa aagcggagca actcctgaag ataatacaag
    19501 agaaaaaaag ctggaccaac gcttatacag aggcacaggc atcgttcgac gacctgctgg
    19561 tccttcatga attttaccag gccgacgaag ctaccgaaga agaagtagaa cgaaactacg
    19621 ccggaacact ccggcttgtc gaaaacctcg aattccgtaa catgctcagc aatgaggaag
    19681 acaagctgag cgccatactg cagatcaatt caggcgcagg agggaccgaa agccaggact
    19741 gggcccagat gctcatgcgt atgtacctga ggtgggccga acgcaaccgg tacacggttc
    19801 gcgaacttga cctgaacgaa ggcgatgtag caggtatcaa atcggtgtcc ctcgaaattg
    19861 aaggagacca tgctttcggt tacctgaaag gtgaaaacgg tgttcaccgg ttggtgcgac
    19921 tatccccctt cgacgccaac aaccgccgcc atacttcctt cgcttctgtc tatgtttatc
    19981 cggttgtaga cgaaaatatt gatattcaga taaatccagg cgatattacc tgggatactt
    20041 tccgcagcag cggcccgggc gggcaaaatg taaacaaggt ggaaacggca gtccgcctcc
    20101 gtcacgagcc cacaggaatc atcgttgaat gccaggtaac caggtcgcag cttcagaacc
    20161 gcgaaaaggc aatgcaaatg ctgaaatcac aactttatga aatagagttg cgcaaaaagc
    20221 gcgaagccca ggcggtgatc gaaggcaaca agaaaaaaat agaatgggga tcgcaaatcc
    20281 ggagctatgt attgcatcca tacaaaatgg tgaaagacct gcggaccgat tacgaaacat
    20341 ccaacgcctc cgaagtactc gacggcgacc tgaacgactt catcaaagcc tacctgatgg
    20401 aatacgggcg cgaaggagaa taggccagcg gaagctatat attagccctt cacagaaggg
    20461 taaaagggat caccgcaggc aagtaatttt attaaattcc ggaccggaag taaccgtcag
    20521 gggggagtac cggtcatatc aacgttgccg ctgtggtttt aaattcaggt tggtataatt
    20581 taatgcctgt tatgcttata ttcgcatgac tcaatctgca ggggcctgca cacacgtatt
    20641 gttcgcaatc gctattcacc gtcatttcca tttcatgaat cacccgaata tgatcgccat
    20701 ttatcataac cccagatgcg ccaaatcgag agccgggctg gagtatctga agaatcaggg
    20761 cgtcgatttt gaggttattg aatacctgaa acatcctttt acccgcgaaa gcctgaaaga
    20821 tctcctgatg aaactaaacc tggggcccag ggatattctc cgcacaggag aggctgtata
    20881 taaggagaaa ttccgtggta agaattttac cgatgaagaa tggattacca ttctgctcga
    20941 aaacccgaac ctgatccggc ggcctatcgt ggtgaacgga tataaggcgg tagtggctca
    21001 accgccaaat gaaatagaca gacttttatc agaccatgcc ataaatcaat attgtcattc
    21061 ctgaaataaa aaccttagcc tatgaaaacc cgcatcgaaa aagacaccat ggggccggtt
    21121 gaggtccctg ccgacaagta ctggggcgcg caaaccgaac gctcaaggaa taatttcaag
    21181 gtcggccccg aaggttccat gcccagggag attatcaggg catttgccgt actgaaaaaa
    21241 gccgcggcca tgaccaacat ggatcttggc gttctggcgc ctgagaaatg tgagcttatc
    21301 agccgggttt gcgatgagat ttgcgaaggc aggctcgacg gggaatttcc cctggtaatc
    21361 tggcaaaccg gttcgggaac ccaaagcaac atgaacgtca acgaggtgat cagcaaccgg
    21421 gcccatgtga tggcaggagg tattctaggc gaaggtaaca gcccgctgca ccccaacgac
    21481 gacgtgaaca aatcgcaaag cagcaacgac actttcccta cggccatgag catcgccgca
    21541 tataaaatgc tggtcgaaac caccctgccg ggggtggaat tattacgaaa tacgctggct
    21601 gccaaggccg aagaattcga caaatatgtc aagattggcc gtacgcacct tatggatgcc
    21661 acgccgctta ccctgggaca ggaattttca ggctatgttt cccaacttga tcacggtatc
    21721 cgggccatta aaaactcgct tcctcatctt gcagagttag ccctgggcgg gacagcggtc
    21781 ggcaccggga tcaatgcccc taaaggctat gccgaacttg tggcccggaa aattgcactt
    21841 cttacgggct taccgtttgt aaccgccgaa aacaaatttg aagccctgtc gagccatgat
    21901 aacattgtgg aagcttcggg cgcgctcaaa gtccttgcca ccagccttat gcatatttcg
    21961 aacaacatca ggatgcttgc ttcgggaccg cgttgcggca tcggcgaact tcaccttccg
    22021 gaaaatgaac ccggttcgag cattatgccc ggtaaggtaa atcctacgca gaacgaatcg
    22081 ctcaccatgg tttgcgccca ggttatcggt tgcgatgccg ccatcacagt tgccaatacg
    22141 cacggacatt tccagctcaa cgttttcaaa ccggtgataa tttccaattt cctgatggcc
    22201 gcccggttgc tcggcgacgc ctgcgcctcc ttcagcgaac attgcattgc cggaactgaa
    22261 gccatcgaac ggaacatcga aaaccacctg gaaaattcgc tgatgctcgt tacagcgctc
    22321 aacacgcaca tcgggtacga aaattccgcg aaaattgcta aaaaagccca taccgaagga
    22381 accacccttc gcgaagcggc tctggccctg gggctggtta ccgatgaaca attcaccgag
    22441 tgggtggacc cgaaaaagat gctgggacga taaaggataa ccggaatccc gaaacccgac
    22501 aaaaatgata ttccgggaag gatatctttg atttttacat ttttctgatg ggaggaaacc
    22561 actccggcaa aaaaccgtac ttttgcccgt caaattattc acggaatgta gaaatcatcg
    22621 ttatacgatt taaacagggg ctcatcaatg attcagacaa taaaaatcgg caccctcaga
    22681 tggcattata tccaggaccc ttccgatgaa gaccttggct ttctgaagaa caacttccac
    22741 tttcaccccc tcgacatcga agactgtaaa agtgtgaatc aacgccccaa aattgatatc
    22801 tatgacgatt attatttcct cattctccat ttccctcatt acgaccggtg gaatcgtttc
    22861 ctgaaaacca aggaagtgaa aatcttctgg ggtaaggatt acatcatcac catcggtaaa
    22921 agccactggg tggtcagcag tcttttcaac agcgccaacg aacacagcga tccttatgaa
    22981 atcctgcagg tcgccacatc cgacgccctg ctttacaaga ttctggaaaa actgatgcag
    23041 gaaacccttt ccctcatcag caagctgggc attgaactgg aactgatcaa ccgtgaactt
    23101 ttcagcaaaa aggccgaaaa aacgattgag cgtatttcgc tgacgaggaa aaacatcatt
    23161 cacctcaaca ccatttttaa acctcagctc cgcctgttcc agaaatttga gagtggacag
    23221 attaaaggct atgccgaaga catggaagaa tactggggca atattctcga ttactaccag
    23281 aaaatgtggg atatgatcga agattatgaa gagttaatcg aaggcctggc caaaacgttc
    23341 gattcgttac agacaaaccg gaccaacgaa atcatgaaag cgctgaccat catatcggtt
    23401 atcatcctgc ctctcaccct catcgccagc atgtacggca tgaatatagg cctccctttg
    23461 caaaaccacc cgtacttctt ctggatgctg atgggcttta tggttgcact ttcaggcttc
    23521 ctgatcctgt tatttaagaa aaagaagtgg ctgtaagtat atgtgagggt tttgcctcag
    23581 aaaaatgatc tgaacgctgg tccttgagat ttgaaagtca tttattcctc ttcctccttc
    23641 aaacccatga tctcttcctg tttcctgatg ctttctttat gcaccatctg aagtagtttc
    23701 aggaggaact ccttgtcaag ccccagtttt gatccaacgt caaggcgttc ggaaatcata
    23761 tcggcccagc ggcgtatctg caatacggtg atattgttat cgcgtttgta ctggccgatg
    23821 tcttccacaa tcttcatccg cctggcaagt atatcgatta attcggcatc aattttgtca
    23881 atctcctgcc tcaactgctc aagcagcgat tcaaattcag gtgtacccga gatattacgg
    23941 acgctcaaac ggctgataag tccggcaagc tgctgtggcg tcacctgctg attagcgtca
    24001 gtcaaggcgt gtaacgggtc aaaatgtgat tcaatcatca ggcccgacat tcccaggtcg
    24061 agcgcttttt gcgacacgcc ctgcaacaga tcgcggcgcc cgcagatatg actgggatcg
    24121 caaacaatcg gcagaccggg cgccagtcgc tgcaactcaa tgggtacttc ccacatcggc
    24181 tggttccggt ataaggcatg cctgtaaaaa taaaaaccgc ggtgaacagc cacaattttt
    24241 gaaatcccgg ttctgtacag cctttcaatg gcgcccaacc ataattttac gtcaggattg
    24301 agcggattct ttaccaacac cggaacatcc aggcctttca aggcttcaca tagttcctgc
    24361 acattaaagg ggtttacaac cgtccgggcg ccaaaccaca atatatcgac accatgcttc
    24421 aaggcatttt caacatgatc cggttttgct acttcgacgg ccgtcagtaa accggtttct
    24481 tctttcactt cctgcagcca tttgagcgct tcgtttccca ctccttcaaa cgacgaaggc
    24541 cttgtacggg gtttccagat gccggcacga aatactttaa cctgtgggat tttcgttagt
    24601 tcccgggctg tttccaggac ctggtcccgc gtttcggcgc tgcacggccc gctgattacc
    24661 aatggcttat cagcaaccgg cagccattct tcaagtgtcc gtattgtaaa attattttgc
    24721 atacctgtaa ggatagtaaa agtaaaactt ttcattcagg gggaaatccc cggaaagaat
    24781 aaaataaccg ccggaaaccg taacctggta gaatgcaacg gaatccggaa atattcttaa
    24841 atttgcttcc tggcaaggaa atccacaata gattaatagt gaatatttta tgagcgcaat
    24901 gaagagaacg aagattgaac aggtactgaa aaactattcc cagcttggca ttggcagcat
    24961 cctgaatata aaaggatggg tgcggacaaa acgcggcaac aagaacgtgg cgtttatcgc
    25021 cgtcaacgat ggttcgacca ttcacagcat tcaggttgta gccgatctgg ccaattttga
    25081 cgaagaactg atgaaactgg tcaccaccgg atcgtgcctt tcggtcaccg gcaccctggt
    25141 tgaatcgccg ggatcgggac aagccgtgga aattcacgcg gctgagatcg aagtttatgg
    25201 tactgccgac gccgacactt acccgttgca gaaaaaaggc catacgcttg aattcctgcg
    25261 tgaaattgca cacctccgtc ccaggaccaa taccttcggt gcagtgttcc gtatccgtca
    25321 ccatgtatct tttgctattc ataaatattt caacgataag ggattctgtt accttcacac
    25381 gcctgtcatt acaggaagtg atgccgaagg cgcgggggcc atgttccatg ttactaccct
    25441 tgacctcgga aacattccaa aaactgaaga cggaaatgtt gattacacgg aagacttttt
    25501 cggaaaaagc accaacctaa cggtttccgg ccagcttgaa ggtgaactcg gggctatggc
    25561 gctttccgaa atatacacgt tcggccctac cttccgtgcc gaaaacagca acaccccgcg
    25621 ccaccttgcc gaattctgga tgatagagcc tgagatggcc ttttacgata tcaacgataa
    25681 catggacctt gccgaggatt tcgtgaaata cctcatccgg tacgtgcttg accattgttt
    25741 cagcgacctt gagttccttc agaaaatgta tgacaacgaa ctcatccagc gtcttcattc
    25801 cgtcaccgaa aatgaatttg aacgcctcac ttatacgcgg gctattgata tactcctggc
    25861 ttccaagcat aaattcgaat tcccggtttc ctggggcatc gatttgcagg ccgaacatga
    25921 gcgttacctt gttgaacaac actttaagaa accggttatt cttaccgact acccgaaaga
    25981 gatcaaagca ttttacatga agcagaacga cgacgggaaa accgttcgcg ccatggatgt
    26041 gctcttccct cgcatcggtg agattatcgg cggatcgcag cgcgaagaaa attatgaaaa
    26101 actcaggcag cgtattgttg aactgggcat tcctgataaa gatatgtggt ggtatcttga
    26161 aacccgtaaa ttcggatcgg ctccccacag tggcttcggt tttggtttcg aaagaatgat
    26221 tctgtttctt accgggatgt cgaatatccg cgatgtcatt cctttcccgc gcactcctca
    26281 aaatgcagaa ttctaaaaaa tccgcataga ttttaaagac caggcccgtt catctttaag
    26341 gcttcaggga ggtcttccgg cgatatttat gattaacttt gattaacaaa ttcgtaatcc
    26401 tcattcaatg ctcaaccaac gattacagca acgaatgttg cagaaacttt cgcctcagca
    26461 gatactgctg atgaaacttc tgcagatacc gtcgttggcc cttgagcaac gcatcaaaca
    26521 ggagatagaa gagaacccgg tccttgaaat ttctgacgaa ttcggtgatg aaacgcctcc
    26581 cgataccgat aatgaacccg acgatgttcc ggccggagaa acggcaacag atgatgaagg
    26641 accggacaat gatataaatc ttgccgatta cctcgatgat gatgaaatac ctgcttacaa
    26701 acttaatgca ggaaaccagt cgccggacga tgaacgtcgt gaaataccct ttgctgtcag
    26761 ttcttcattt caggaatcgc tgatcgcaca gctcggcctg catagcctta ccagggaaga
    26821 taatatcatt gccgagaata tcatcggcaa cctggatgac gccggttacc ttcaacgcga
    26881 cctgaatgcc atggtggatg acctggcatt cacccaaaac atcagcacga accggaaaga
    26941 gattgagcgg atattacaaa ttgtgcagga actggatcct cccggaattg ccgcccgaaa
    27001 tcttcaggaa tgcctgctcc tccagttgag acgcctggga acggttccgg gtatcaaact
    27061 tgccatgctc attatcgaac gctatttcga cgattttatc agaaagcact acgataaaat
    27121 catgaaacgt tcgcattgca gtgaacagga cctgcgtgaa gccgtcaaca ttatcctgag
    27181 cctcaatccg aaacccggga atgccatcag cgaacaaggc agggacaacc agtacattgt
    27241 tccggacttt atcattgtaa acaaggacgg cgaactggaa ctcacgctta atcagcgaaa
    27301 catgcctgaa ttgaggctca acaggaccta tgttgatatg cttgaggctt atggtgaaaa
    27361 taaaggccgt aagagttccg gcagccagaa agaagcggta atgttcatca aacagaaaat
    27421 agattccgcc aaatggttca ttgatgccat aaaacagagg cagcatacgc tttacgttac
    27481 catgaaagcg atcatggatt accagtataa ttactttctc gatggcgatg aaacccgtct
    27541 caggccaatg atcctcaaag atattgcaga aagggtaaac ctcgacattt ccacaatttc
    27601 cagggttgcc aacagcaaat atgttcagac acctttcggg acattcctgc tcaagagctt
    27661 tttctccgaa gctatccaga atgaccaggg cgaagagatt tcaacccggg aaataaaaaa
    27721 aatactgtcc gattgcattg aatcggagga aaaaaataat cccctcaccg acgaagaact
    27781 taccgccata ctcaagaaaa aaggctaccc gatcgcaagg cgaaccgttg ccaaataccg
    27841 cgaacagctt aacataccgg tcgcccggct aaggagaaac atctgagttg aaacattggc
    27901 ctgctcgttt tttttcgaaa tggccttcct gcctgcatta ccccataaat cacctgaaat
    27961 ttcatggaaa cccgaatagc ccgcatcctg tcctatatac tgcaccccct gctgctcccg
    28021 acgttaagtt tcatactcat cggcacattg ccgtccatct tcgtcctcgg acttccggta
    28081 aaagcgcaat tgtggctgct ggggatggtt tttacgttta ctttcctgat tccggttaca
    28141 ggaacattca tgctcgtaca atttgaccgc gtacggtcca tcgaacttaa agaatcgcgg
    28201 gaacgtaccc ttcccctgat gataacaggg atctcgtaca tgtttctgta ctacctggtg
    28261 aaggatgctt caataccggc catctacctg ttttttatct atggttccat catcgtcatg
    28321 gtagccggca tgctgattaa cctgttctgg aaaatcagcc ttcacacgct ggcctgggga
    28381 gccgccacag gagcactgac aggcatttcg ctccgtttta tgctcgatat tcccctgacc
    28441 atcgcctgca cgatcatact ttcaggcctt gccgggtttg cccgtttaaa actcaacgcc
    28501 cacaacccaa cccaggttta cgtgggtttt gtcgtgggcg ccgctatgat gcttttgctt
    28561 atccttttat tttgagccga aaggcataaa agtcagtcct accgaaatcg ggtaaacttc
    28621 agggccataa tctttctcaa agagcgttga aatctggtaa ttggccatga tgccgaacca
    28681 cttatagccg aaggaaatat aggggccata atggaaatta tcgatattgg gcaccctgct
    28741 gtattttcgt ttgatggtag caccggtggc gtacagaaca ttatccgctt cataacaatc
    28801 acccttatac ttccaatggt cgttgatttt ccagccgact ttaaaaccca gggtcaagtg
    28861 catgtcattc ttcattttat atttaaaacc aaaaggaaca tcaaaataag tgacggaaat
    28921 tttgcttttc tttacatcaa tggtggtacc tccaagggta tcgggaagat tatataaata
    28981 agtaataccg ccatcattca gaccgataag ggcattatga aaaaagttat gggcgccgat
    29041 gcctgctccg acaaaaaacg aaatatggtc cttcctgtcc atcggaaaca tataaagccc
    29101 gtaaatatta acgccctggt tgatagtcct cggattgatg gaatccggga catccatcca
    29161 gatgtcgtta aatggttccc agctcagcct gaaccttttg gttatcgatt ccaccgacgg
    29221 cttttgagaa aaagcagcaa ctgaaaacag cgacagaaaa acaacgacag caatacgttt
    29281 catcgaaaat tggttttagt gtatatgccg caaaagtaag tgaaaatccg gctcaatgag
    29341 tgatttgtcc cattcctgaa aaaaaccgcg gccaaattgg caaaaattaa cagttgatgc
    29401 agaaagtttt ctccaaaatt gtaaatttgc actcagaaat ccgcttttgg cggtagactc
    29461 tgaagcagag tttaagggcc tatagctcag ttggttagag caactgactc ataatcagta
    29521 ggtccctggt tcaagtccag gtgggcccac cacgaaggtt aacgaaatgc ttgattttcg
    29581 tgcaaattgt taattttcag acgtttcaaa atgaatagct taacgttttg aaacacctgt
    29641 gttccttgat tagcggcgtt tttgcaacaa aatttcaaca aaaattttaa cgtaataacg
    29701 ccatgaacac ttatcactct caagtaaaat tacgtaccga ttctcagaga aaagacggta
    29761 catgttcgct tcatctttat gcgaacatta acgggaaaag aatgttcttt tcccttcaca
    29821 aatcaattcc tgaaaagtac tggaatcctg acaaacagga agttaaaatt gtctgcccca
    29881 acgcagttat tattaactcc accatcaagg attatcttca gaaagctgaa aaactaatgt
    29941 tatctgcaaa tatcaacagt aagatcgtaa gcaaatctga tcttgaagac attttgcgga
    30001 gtgaacccta tgatagtaat gacttcttcg cctttgccga aaacgacata aaggaattcg
    30061 gaagtaaatt cgcacctgct accattgcca acaataatgt acaactgaaa aagttaaagg
    30121 aattttaaaa caaagtcacc ttccggaaat tcacaccgtt cttttggcgc aggtatgaga
    30181 agcacctgca agcgatgggt aacaatccca acaccgtcta taaagcgttt aaaaccctga
    30241 aagtttttat taaccgtgcc attgaacagg aaatcatcga aaagaatcct gtcttggagt
    30301 taaggttcga cctgctgaag gtaaaatgca atatttgacc cgtgacgaga tcaagaactc
    30361 gaaaaattcc acgaagggct gacgatacct gcatataagc gggtacttca gtgttttttt
    30421 taatttgtgt gttattttca acggaaatta tacaaaacag aaattaattc ttcgagattc
    30481 ttgctaaatc aagaagaaat tgcaaaacga gtcatatctt tgcattgaat ttcagtttat
    30541 ttgaagtatc gaaaacagcc taattaaaat taaccatcat gttagagcgc cctcccttag
    30601 cctcagaatc agacctgctt gagtcgatga aaacagccta tattcccgaa attcaaaagc
    30661 tcgattcgga atatgcttac tgggacaagg tgaagtatat gacggctcct gataacctca
    30721 cttccaaaca gctttggttt gcaattaagt ttttacgtca atttaaccgt actgttttaa
    30781 aattcggcaa atatgaattc agtttcactg taactgatga gatgtttgag cttctgcatt
    30841 actttgatat gaatatcggc ggtaacctga caggcgaaca aatcatacca tctgagagca
    30901 aaaacgcgta tcttatttct tcgattatgg aagaagccat tgcctccagt caaatggaag
    30961 gagcttccac taccaggaaa gtagctaaag atatgctgcg caaaagcgaa aagcccagaa
    31021 acaaagggca acaaatgata ttaaacaatt atataactat taattacata aaagggcatt
    31081 cgaaagataa tttttcagtt gaccgtttac gggaaattca acggtcaatg acaataaaca
    31141 cactggataa tgaaagcgat gtaggaaata tccgcactca taacaatatt atggtcgttg
    31201 acggaataac cggcgatatt gcgcacaatc ccccttcgca tgaggagctt gacaaattgc
    31261 tcaacgatct ggagttgttc tttaataaaa acatgggctc ttttattcat cccatcatca
    31321 aagccatcac ggtacatttc atgttgtcct attttcaccc gtttgttgat ggaaatggcc
    31381 gcacggcgcg ttcacttttc tattggtata tgatgaaaca gggctattgg ctgattgaat
    31441 tcatgtcggt ttcgcgtatc atatacaaaa ccaaagtgat gtacgaaaag gcatttctgt
    31501 acaccgaata cgacgataac gatttgacat attttttgtt gtataatctc cggacaatga
    31561 agaaagcgtt tgaagagctc aagctttatc ttaaaaggaa aacagaagaa agcagcagcg
    31621 ttttaatgct ttctcatatc aaagggatca accagaggca ggctcagatt ttaaaaattg
    31681 tacaggagaa acctaattca tttttttcgg taaaagaaat tgaagtccgg ttttcagttt
    31741 ccaattttac agccaggacc gatttggatg gccttgtaaa acttggatat ttatctgagg
    31801 taaaaataaa caaggtgaaa aggaactata taaaatcgga caggttcgat gcgcttatta
    31861 aagaaaacaa tgttagaaaa agataacgaa gtttcaatat tcttgatgtt tcaagaagaa
    31921 tttttaaaga accgtctcta aaaccttttc atgttaatca atacagtatt gttgccgaaa
    31981 ctccggaaag cacagtaata accgaatacc agcggctata ccacaaggaa acgacctttc
    32041 agactgaatc ggaactcgaa caatttatgc cggcagatca aatcctgagt tttatctttg
    32101 cagcggcaaa gcattaataa acgctgaaga gaaatagttg agccctgttt ggcttgcagc
    32161 tttaaagcca tagacttagc cggggccagg cattctcccc ggcaaattca cattatcctg
    32221 atgaacacaa tcgacactat aattttcgat ctgggcggcg tgttgtacga tatcgacgtt
    32281 gatcatacgg ccagggcctt tactgagatg ggtgcaggcg aaataaatat ggaagcgttg
    32341 cacaaatcga tgcatgaaga gcatatttat gaagggctgg atacgggacg gttaagtcct
    32401 gccggtttca gggatgaaat ccggcgcctg acggggatgc cgctcagcga cgaacaggtc
    32461 gacgctgcct ggaatgccct gctggttgaa ttccccgccc gccgcgtgaa aatgatgctc
    32521 agcctccgga agaattacag gttattgttg ctgtcaaaca ccaatgccat ccatttcgat
    32581 tactattcaa agcatttcga aacggactat ggcattcctt tcgaaagtct cttcgataaa
    32641 gcatattatt ctttcaggat cggactatgc aaaccggatc ccgggatata ccgacatgtt
    32701 atcaccgata gcaaactgga accggagaaa gccgttttta tcgatgactt attcgttaat
    32761 gtggaagccg ccaggaaaac cggcctgaag gccatccatc tgaacggcat tccggatgta
    32821 acagaccttt tccgggacaa ccggcttgat acccggttcc tgaaaagtta aagaaattga
    32881 cggctttgca ggatcactcc tgcaattccc ctgcatcatt cttcatcata cgatatatcg
    32941 tcgttttgcc gatatccagt ttttcggcta cgatccttac tttattatcg tattttttca
    33001 ggtagtgcat gatgatacgg cggtcgtatt cttccagggt ggcttcgccc gatataaaat
    33061 cgaaaggttt ttcaatttca ttgatcctga tatcctcggc atgtatggtg tcgctgttgg
    33121 tcataatagc cgccagttcc acgatggctt tcaattcgcg cacatttccg ggaaacggat
    33181 atttcttcat tttctccatg gcgtcggctg aaatggttaa acgctgaatg ccatttttat
    33241 cgcaaaactc atcaataaaa tgcctggcaa taatgaaaat gtcattgtcg cgctcccgga
    33301 gaggcggtat ttcgataggc aatccaagca atcgataata caggtcttcg cggaaagtcc
    33361 cctttttcac ctcttccagc agatttttat gcgtggcgac aattacccgc acatccagtt
    33421 tcaccggctt gttcgaacca accctggtta cttcctcttc ctgcaataca cgtaataact
    33481 ttgcctgcat gttcaggtcc atatcgccga tttcatccag gaagatggtg ccatgatttg
    33541 cttcttcaaa cttcccgata cgctgggcgt gggccccggt aaaggcgcct ttctcgtgcc
    33601 cgaacatctc gctttcaata aggtcgtgcg gtatagccgt tacattaacc ggaacgaaag
    33661 gtttattatg cctctttgaa ttgtaatgga tggattttgc caccaactcc ttcccggtcc
    33721 cggtttcgcc ataaattgaa accgtaatat tggctttaga ggctttttca agcatctgaa
    33781 ataccttgtt gatagcaggg ctgttccctt ttatcaggtt gctgtatgag tatttcctgc
    33841 cgatttcttc ttcaagccgg gtgatttttt gcttcaggtt gaagttttcc ttgatattcc
    33901 tcatcaccga ccagagatgg tccttggtgt cggcatcttt cagcacataa tcgtaaacgc
    33961 ctttcttcag cagttctaca gccgtcgaaa tgtcctgctg tcccgatacg atgacaaccg
    34021 gaagttccgg atcgaattca cggatttttt tcagtacttc aaatccgctc atatcgggca
    34081 ggttaaagtc gagcgatatt accgaaggcc ttttgtataa attcagcaac aaatcgtggg
    34141 cggtcgaata aacatgcacc tcgtagtcgg ggttctgcga caggtggtac tctaatattc
    34201 tcgcataaag cggctcatct tcaacaatat aaatccggaa aggctccata cactcctgat
    34261 attaaatacc ctgatgagaa caaatgtaca ataatcaaaa cagtttccgc aattgggaca
    34321 aaaaaaaata ttctttcaat ttgaaaaatt gttttattct ggaccggaat caggataatt
    34381 ctgcttgatt tcggacagca atccgatgat ttctgattca gacttcaggg taagcagttg
    34441 gatacggtac tttttaaaat cgtacattcc cctgaaataa ccggcataat gcctccgcat
    34501 ttcaaccacg gcacgatgtt cccctttatt ttcgaccgct ttgtgcagat gccgcaggca
    34561 aaccgaaacc ctctccccta ttccggtcgc cggcattaca gcggaccgat ttgatcggaa
    34621 accggatatt tctttgaaaa tccacggatt gccgattgcc gcccggccaa tcatcacggc
    34681 atccacgcca tacttttctt tcatgagaaa aactttctca gccttgtcga tatctccgtt
    34741 tccgattacc gggatgctca ttctcggatt gtttttcact tcaccgatca gcgtccagtc
    34801 ggctgtgccg gaatataact gggcccgtgt ccttccgtga atcgtgattg ccgcaatgcc
    34861 ttcatcctga agccgttcgg caatttcaac tatattctta tgtgaatcgt cccatccaag
    34921 cctcgtcttg acagtaacag ggatcgaggt tgctttcacc acttcgccgg taattttaac
    34981 catgcgggac acatcgttca ataatccggc cccggcgcct ttcatcgcta ctttctttac
    35041 cgggcatccg aaattgatat caatcacttc cggctttgct tcggcagccc gtaaagcggc
    35101 cagccgcatc gcctccggat ctgatccgaa aatctggatg ccgatcgggc gttcgcactc
    35161 ttcgaaaagc agtttgtcgt tgcttttccg ggcatcacgg acaagccctt cagaagatat
    35221 gaactcggta taaaccatcg ctgcaccgaa ttccctgcat atcaccctga aagcagaatc
    35281 ggtaatatct tcaagcgggg ccagcaatac cggaaattca cccaaatcaa gcgagccaat
    35341 cttcattttc ctgttttgca caaaggtaac caatcattct ttgcactatc gttagcatcg
    35401 gctgtttgta ttgacgatga cggaagccgg cgaacactgt tgccgctatt cgttattagt
    35461 gcctggagta cttggagcac ttggagtgcc tggagtgcct ggagttctga gttgtgagtt
    35521 accttttccc tttgcctttt gacctttgac cttttgacct tttgaccttt tccttctggc
    35581 ttttagccat tggctcttgg tctttggcgt tttgagttct gagttttgat gggacagttt
    35641 gggtttcgtt gttagttatt cgtgggcaaa gtttcagggt ttcagacctg cctgcctggg
    35701 aaggctgtgc gtcaggtggc aggcctgcct gcc
//
LOCUS       DAOPYV010000029        34605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_1159_length_34605_cov_11.729725, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000029 DAOPYV010000000
VERSION     DAOPYV010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 34605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34605
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1159_length_34605_cov_11.729725"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1848)
                     /locus_tag="PKI35_05520"
     CDS             complement(<1..1848)
                     /locus_tag="PKI35_05520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX43877.1"
                     /translation="MKLSVVIVSYNVRYFLEQCLHSVITAGQDMEMEIFVVDNASVDG
                     SVKMLKEKFPEVHCIANTENVGFSRANNQAIRQSTGDYILLLNPDTVVESDTFRKTVA
                     FMEEHPDAGGLGVKMVDGTGHFLPESKRGLPKPMVAFYKIFGLARLFPRSRTFNRYHL
                     GYLDDGQVNPVDVLAGAFMMLRRETLEKTGLLDEQFFMYGEDIDLSYRITLAGYKNYY
                     FPETRIIHYKGESTKKGSLNYVFMFYNAMIIFAGKHFSRKNARLFSILINMAIYFRAS
                     LSVVHRAARTLLLPLLDTALIYTGICLITRYWEHTVIYPYGGHYAPAFYFYVLPAYIL
                     LIILSVYVSGGYDKPVRLGRVVRGVGAGLVITLVVYALLPLQFRFSRALIVIGSMWGL
                     IAMTGLRLLLHLFKISGYVLNVSKSRRFAIVGNADEAHRVSDLLQKAVYKPAFIGLVR
                     AGDLNGEKNGFLGGIKQIRDIITIYDIDEIIFCAKDLAAEVIIDHMSELQDLQVDYKI
                     APPESLSIIGSNSINTAGDLYVIDINSIGKLENRRSKRIFDVFAALILLVSYPVSAFF
                     TRNPFNFLLNIFRVLFGFRSISGYCPTKVQDFRLPSIRKGILNPADALRA"
     gene            2353..4380
                     /locus_tag="PKI35_05525"
     CDS             2353..4380
                     /locus_tag="PKI35_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNX43878.1"
                     /translation="MIPIAAFFAIILVTLILIPFLKVRLKGIITFLAVVLNAAITGYY
                     AVLSLSGQTFLYELPGSLFTGSIPLRLDALSGWFILIINFVFLTGGFYGFFYLKAYKS
                     QVHNLGLHAICFLIQHASLLSAVIVQNSIAFLIAWEMLALTSFLLIIFEREKETTIAA
                     GLNYLVQSHFSFIFLVIGFIWVAQKTGSYDFNAVTAYASSLPVGASMLLFMIFFTGFA
                     FKSGFVPFHTWLPHAHPAAPSHVSGMMSGVVIKAGIYGILRMLLLIKTDYTTIGYIIL
                     GVSVISGLYGVMLAIVQHDLKKLLAYHSIENIGIIGLGIGVGCIGMGAGNYLVAMLGF
                     AGALLHTLNHALFKSLLFYAAGIVYQATHTLNIEQLGGLIKKMPQTAALFLIAAIAIS
                     GIPPFNGFISEFLIYSGLYNWLQGASLASLICCLFMIMGLVLIGGLALLCFTKAFGIV
                     FLGNPRHAFHHEIKEAPFMQLLPLYLIAFLIVSIGLFPGYFLNMIMAPVNLFPGIQGV
                     AGTLFRENTAGIMQNISWAMWGLILLTSLIYLVRKLAMQKREVAADSTWGCGYVAPTA
                     KQQYTAGSFVKTYSQLNRATLSIKKHEKRVDGLYPGEAHYETHAYDQLEKWLIDKPLD
                     GIRTFLGWFRFFQNGRLQSYILYGIVFILALIFLPMIFDSLSGFIDFLKHL"
     gene            4383..5288
                     /locus_tag="PKI35_05530"
     CDS             4383..5288
                     /locus_tag="PKI35_05530"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNX43879.1"
                     /translation="MLSLVLILIAAVFFTGIINRTKSIFSGRKGPGILQPVKDVIRLF
                     RKGAVYSRTTSFIFQMAPAVYLSSVVMAMLVVPFGQSKGVISFSGDFIFFAYVLALGK
                     FFNIIAALDTGSSFEGMGASREALFSMFAEPAFFILIGSLALLTGYTSFYEIFAAFHV
                     GSPVSYALAVLAAFVLIMITMIENSRMPIDDPKTHLELTMIHEVMILDYSGFDLGLIF
                     TAGLLKFAIYGALIVNLFIGIFPYEYSIPMFFAIQFVTAVLIGAVESFMARFRMNHNA
                     QFIFALTSVSLLIFLGALLILGKFV"
     gene            5307..5936
                     /locus_tag="PKI35_05535"
     CDS             5307..5936
                     /locus_tag="PKI35_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43880.1"
                     /translation="MINVLLITFLITLFYVAIANRLLTYVKVLAFQGVLLFVVSFIQL
                     SEINTLNLVLILMETVFFKSVAVPLFLAYVLKRNKITREAEPYVSNFISLIIVTFICV
                     ISILLVNSIQDTESDKIFYVVALAALFTGIYFIISRRKIITHVMGYLIVENGVFILSL
                     AVGDKMTMLVNIGIMLDIFVSVLVLGIFANRIGDTFEAMNVDNLTNLKD"
     gene            5942..7372
                     /locus_tag="PKI35_05540"
     CDS             5942..7372
                     /locus_tag="PKI35_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNX43881.1"
                     /translation="MFQYYLILVLVLAAGVFFNRNRAVRGVLLGIFLLLQTSVTIYGY
                     YHIGGQEADGYFSFDALGLIFLSVLTILSFTTILHSLVYLKHREDDWRRQRIYFTALI
                     LLIGAMTSVYLASAIGIMWVLIEMTTLSVAALIYHERTHLSLEATWKYVFICSIGITF
                     AFVGIVFLSKALQEGGSVNLAFSDIPLHLQNAGAFWLKLAFIFMVVGFSTKMGLFPMH
                     TITVDAHTVAPPPISAFISTTLMNVGFVAIFRTYTLMSHTCILPWMNQVLLWSGILSV
                     FISTVYILKADHIKRMFAYSSLEHMGLSALGLAAGGIGYFAAILHMVLHSFIKASVFY
                     QIDQVHRTFGTYVISKTGNYFNKNLAGALVMLFVYISITAIPPSGMFVSELLVFRSLF
                     ESNHIIVLILILLLLTMIIWSMGKNIFKMLFVPGKDADATIKADIKKWESVSQFILLA
                     LGVYLGLNPPSGFVQLIRDAVMLLPQ"
     gene            7374..8870
                     /locus_tag="PKI35_05545"
     CDS             7374..8870
                     /locus_tag="PKI35_05545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNX43882.1"
                     /translation="MKYITIHNNQAVPVSEIPESDYEAFLELNAGFVQQSVERHCVSY
                     FGYVSGAGVKLVCCIADDQSHEIYVSSSLVQTDKKLPSFTARHTCFEKFERELHENFG
                     ISYSDHPWMKPVRYAHDRYDKGDTIGDYPFYSIESEELHEVGVGPIHAGVIEPGHFRF
                     ICNGEKILHLEIQLGYQHRGIEKLFVEKKPLAQLATLAENIAGDTAVGHSVAFAQLWE
                     CLSGFETSRDVDYARTIALELERIAIHTGDLSAVCTDIAYQLGSSVFGRLRTPIINFM
                     QEWCGNRLSKGLIRPGRNKYPFTQALAGRLTEVLDRFEPDFIEMNRELFLLPSALSRF
                     ERTGVVTAEDASAIGAVGMAARASGLSRDIRKSHPHALYPELGHEPVVKRHGDVYSRV
                     QVRKEEVYQSVAYLRKLLEDIPGNAEKETLQSPGRDLFALSLVEGWRGEICHCAVTGP
                     DGSLKVYKVKDPSFHNWMALALAVRNNEISDFPICNKSFNLSYCGHDL"
     gene            8888..9655
                     /locus_tag="PKI35_05550"
     CDS             8888..9655
                     /locus_tag="PKI35_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HNX43883.1"
                     /translation="MLNNLKILFHQGKQYIPDVTTAKVPGIFRGRPVISPEPADGQRL
                     ADVCPTGAISVNPVCIDLGKCTFCGECALMFPEKIRFTTDYKISVTERDQLVVKEGAD
                     QPLVIDPDKVRKEIRKIFGHSLKFRQVSAGGDNSCEWELNACGNVNFDMGRFGIEFVA
                     SPRHADGIVITGPITENMAEPLQICYDAVPAPKIIILAGTDAISGGIFAGSPALDRRF
                     IEKYPIDLFVPGNPVHPLSFINGVLSLMGRKPPVKPQ"
     gene            complement(9882..10643)
                     /locus_tag="PKI35_05555"
     CDS             complement(9882..10643)
                     /locus_tag="PKI35_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNX43884.1"
                     /translation="MRNLKAFLDAKSDEFNTADFIARDPVSVPHRFNSPPDIEIAGFL
                     SATLSWGQRPQIIAKARALTELMDNEPYNFLTQAPEKEFERFSRFIYRTFNGDDCLFF
                     MYSLRSVYREYETMEQLFTEGYRKENSVRDGIITFRKKFLEMPHLVRSAKHLADPGSG
                     SAAKRVNMFLRWMVRNDGRGVDFGLWKRINPDHLMCPLDVHSGRIARMLGLLKRKQDD
                     WKAVEELTANLRLFNAGDPVKYDYALFGTGVNGMH"
     gene            complement(10727..11281)
                     /locus_tag="PKI35_05560"
     CDS             complement(10727..11281)
                     /locus_tag="PKI35_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNX43885.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKILAYSGVMQNKEVSWMPSYGPEMR
                     GGTANVTVIISDERISSPILNAYDTAIILNQQSMDKFESTVKPGGLLIYDPNGITRHP
                     ERKDINVYTIEAAEKAGALGMSKVFNMIVLGGFLKLKPIVSMEFIHKGLEKSLPARHH
                     NMIPENEKAIELGKDLVNAVTILN"
     gene            complement(11329..12093)
                     /locus_tag="PKI35_05565"
     CDS             complement(11329..12093)
                     /locus_tag="PKI35_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNX43886.1"
                     /translation="MMNAEIVKPENLVYKRTELLTPRTLHYCPGCGHGTTHRIIMEVI
                     EEMGIQSQTIGIAPVGCSVLAYDYMDIDMQEAAHGRAPALATAVKRLNPGKFVFTYQG
                     DGDLAAIGTAETIHACNRGENIVIIFINNGIYGMTGGQMAPTTLEGMKTATCPYGRDI
                     AMMGNPLKITELVAQLPGTLYVTRQAVHTPAAVRRTKKAIRKAFENTKLNKGLSFVEI
                     VSNCNSGWKMTPRQANDWMVENMFPFYPLGDIKVNE"
     gene            complement(12109..13191)
                     /locus_tag="PKI35_05570"
     CDS             complement(12109..13191)
                     /locus_tag="PKI35_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNX43887.1"
                     /translation="MRELKLMKGNEAFAEAAIRAGADGYFGYPITPQSEVMEYLMAQK
                     PHERTGMIVLQAESEIAAINMVYGGAATGKRVMTSSSSPGISLKQEGISYIAGAELPC
                     LILNAVRGGPGLGTIQPSQSDYFQSTKGGGHGDYRLLVLAPSSVQEMADFARLGFDLA
                     FKYRNPVLVLSDGAIGQMMEKVELDPQRPRFTDEEIKKNTPWATTGKTSDREHNIVTS
                     LDLESTRMEKHNLDLQAKYRLMEEQEVRYEEISCDDAEYIFVAYGTSARICQKAIQLA
                     RAEGIKVGLLRPITLFPFPKKILNKLAKKTKGMLVVEMSAGQMVEDVRLAVECQVKVE
                     YFGRYGGIIPSPDEVVAALKKQIIGG"
     gene            complement(13215..13439)
                     /locus_tag="PKI35_05575"
     CDS             complement(13215..13439)
                     /locus_tag="PKI35_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX43888.1"
                     /translation="MAKLKGQIVVDTERCKGCEVCIPACPEEVIAMSKHVNHKGYFYA
                     QPVKDACTGCTNCAVVCPDGAITVYRKKVE"
     gene            complement(13590..14921)
                     /locus_tag="PKI35_05580"
     CDS             complement(13590..14921)
                     /locus_tag="PKI35_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNX43889.1"
                     /translation="MKTTAKDTPINGDIVNEKISELNVPVVGKATIREIKRLIDNIER
                     ASGIKFIRMEMGVPGLPPAAIGTEAEIDALRHGVAAIYPDIDGIPELKTEASRFVKNF
                     LDVEVSPDGCIPTVGSMQGGFASFLTLARMHKERDTTLFIDPSFPVHKQQHKVLGQKF
                     EGFDVYGYRGEALRTKLESYFSKGNIHSVLYSNPNNPSWICFTDEELKIIGDLANKYN
                     VVILEDLAYFAMDFRKDYSQPGVPPFQPTVAKYTDNYILFISSSKAFSYAGQRIGLMV
                     ISDKLFHTKAPDLLRYYSSDIFGKAMIFGTVYALSSGTAHSAQYALAAMLKAANEGRF
                     NFVEDVREYGRKANIMKKLFTDNGFRIVYDKDLDQPVADGFYFTIMYPGFDGPELIEE
                     LLYYGISAISLSITGSERHEGLRACVSLVSRSQFPDLEMRLKKFKEHHPVA"
     gene            complement(15177..15872)
                     /locus_tag="PKI35_05585"
     CDS             complement(15177..15872)
                     /locus_tag="PKI35_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43890.1"
                     /translation="MKRKDLVFITLFIILLLPFFIFDSVYSTYVSFNSHHGMIMSFIK
                     FAVLATLGEMIALRIRKGVYHEPGFGIIPRAMVWGFLGLTIKAAFVVFAAGMPLFVQY
                     LGLNDAVQVMQGDLSLMKILVAFCVSAGMNLTYAPVMMTFHKITDAHIIQTGGTLQGL
                     FRPIPFAEIFRNINWDVQWNFVFKKTIPFFWIPAHTITFLLPPDFQVLFAAILSIALG
                     LLLAFAGMKGAKK"
     gene            15955..16824
                     /locus_tag="PKI35_05590"
     CDS             15955..16824
                     /locus_tag="PKI35_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43891.1"
                     /translation="MRFFFLFSFIFLQIAVQAQINTRVKSTKDQDSLARTNSITDTTS
                     FTPESRNPDNPFIDYKPAVIYLTSSDSIEDHAYIRQNFTDQMVSVWSKPNRLYPASKI
                     YGLRMDGKLFRAVRKSAYECVFAERAVHGPMSLYIYTRIPQSSGWVECYSAGGYTNNM
                     IVENKVTRNKNSYGYFVSLYPDTNNFIAADDINKFAESYLKDMPETYKQASPFLKIKR
                     YKAQKQILTLAMLVGVGGVAFIDSNMKWLFLAGFPVAAILTFINRSHIPDWKDMVAIV
                     NSYNRETLTAAGQ"
     gene            complement(16892..18946)
                     /locus_tag="PKI35_05595"
     CDS             complement(16892..18946)
                     /locus_tag="PKI35_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNX43892.1"
                     /translation="MITSQLINPQSIVVIGASNDVTKTGGKALKNLLDHHYPGKLYAV
                     NPKETNIQGLTCYKDAADIPQADLAILAIAARFCPDTVETLAREKNTRAFVIFSAGFS
                     EESAEGAKLEKKIVETCNRYGAALIGPNCIGMMNSNHTSVFSLPIPKFNPKGIDFISG
                     SGATAVFIMETAMQKGLSFASVYSVGNSAQMGVEDVLMHLDETFDPEKSSRVKLLYIE
                     SVNRPAMLLKHASSLIRKGCRIAAIKAGSSAAGSRAASSHTGAMASPDVAVDALFRKA
                     GIVRCYGREELTTVAGIFMHKELKGKNIAIITHAGGPAVMLTDALSEGGLEIPHLESP
                     ELLSKLFAGSSVANPIDFLATGTAEQLGEIIEHVDKKFDNIDGMAVIFGSPGLFDVSD
                     VYKVLDEKMHTCNKPIFPILPSIVNVKQQIDYFISLGRINFPEEVIFGKALCKVYHTP
                     KPQPEKIELPAVDIKTVREVIDNAANGYLSPGEIQQLLDSAGIRRAGEAVVTSKDEAV
                     KAATVLGFPVVMKVVGPIHKSDVGGVVLNVNNCDQVAAEFDRMIRIKDTTAILLQSMH
                     SGIELFAGAKYEPGFGHMVLCGLGGIFIEVLKDVSAGLSPLSHDEAESMIRRLRGIKI
                     LEGVRGQQGVDIPRFADVVVRLSALLQAAPEIMELDLNPLLGKEDIIVAVDARIRIEK
                     NA"
     gene            complement(19165..19365)
                     /locus_tag="PKI35_05600"
     CDS             complement(19165..19365)
                     /locus_tag="PKI35_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43893.1"
                     /translation="MQRNYLVDLVAGQINIYWMVKMAYPRYVSGYPPPDAISGAVGCR
                     QHIPKIPAIFLNNPEMMILQTG"
     gene            complement(19970..21781)
                     /locus_tag="PKI35_05605"
     CDS             complement(19970..21781)
                     /locus_tag="PKI35_05605"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNX43894.1"
                     /translation="MENPKPLVTVLDGRSFYFAFLAGAQRIFENQGLINKINVFPVAD
                     ADTGTNLASTMRSIVDSPIPTDNIKVTASALAEAALVGARGNSGIIFAQFLYGFSNEI
                     VNENNFTIENFARSVRNAVTYAYEAIANPVEGTIITVMREWAEFIYAVKELFDDFVEL
                     LTQAYQKASESLKETTRKLEQLAKANVVDAGAKGFVLFLEGMIDFFKNRQVVRKLDVL
                     NNEISMSEVENFSHEIITFRYCCEALLSLDPKSKDNKGTIKKSIAHLGDSLVVAGSDR
                     KLRVHIHSDDPISLFGILGKKACITYQKVDDMVMQREMSHNRKWPVALLTDSTCDLPQ
                     DILEKYHISVAPLSLHFGESHFLDRTTISSEQFYKLFEESPVLPTTAQPAYKEFINKY
                     SFLATHYDSIIGIHISRHLSGTFSNSERAAKAVSEQSGKKISVLSSNRLTGALGLVVL
                     RAAEAIEKGANHDDLVDQIETWKKKNHILVSAKTVKYLVRSGRLSYSKGMIGKLLGIN
                     PIISMDEDGKATTFGKAFNEKGSMRIIMRQIEGLLKEGKAWGYAITHVHNQATADWYA
                     QQMETLTGMKPRFINDVSPVLGVNGGPGTTVVSILMQ"
     gene            complement(21908..22414)
                     /locus_tag="PKI35_05610"
     CDS             complement(21908..22414)
                     /locus_tag="PKI35_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43895.1"
                     /translation="MKTSYIKALSFLMMSSAIISCCHSGKISQKAAATGIPGPHAIIY
                     QTRNDYSQLVPINLTADKKSVESYPDVKDVYFGGKLALPTQLHDGWLLDNRGISPNSA
                     FISLTYEQYSKLEKTPAAEELLRMVIDKDPVTKIFDCGLRTDYKDVVSELNRHIDEKS
                     FSGFKTLK"
     gene            22674..23156
                     /locus_tag="PKI35_05615"
     CDS             22674..23156
                     /locus_tag="PKI35_05615"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNX43896.1"
                     /translation="MKKVVLSLAVSMLVSVAVFAQQNATISSASKPAANPNAPVITFE
                     KTTHDYGTVTKGGDGTCEFKFTNTGVEPLILSNCTASCGCTVPDWPREPILKGKSSVV
                     KVKYDTNRIGGINKTINVVSNANNSPVQLRIIGNVVDKSAATAYPEKNLNQGAAPVAK
                     "
     gene            23305..24504
                     /locus_tag="PKI35_05620"
     CDS             23305..24504
                     /locus_tag="PKI35_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNX43897.1"
                     /translation="MPTISQRGQQMPASPIRKLVPYAEAAKKRGIKVYHLNIGQPDIR
                     TPEVAIEAIRRFDQKVIEYSHSAGNESYRRKLATYYQARGIQVDHNDMIITTGGSEAI
                     SFAFKVCLNPGDEVIIPEPFYTNYNGFAVDANIKVVPITSDIRSGFALPPISEFEKAI
                     TPRTKAIMVCNPNNPTGYLYSKEELLQLRELVLKHDLYLFADEVYREFCYDGNTHFSV
                     MNLEGLEEHAVLMDSVSKRYSECGVRIGVLISKNKQLMAAALKFAQARLSPPSLGQVI
                     GEASIDTPAEYFKEVYDEYIARRNYVIEALNKIPGVFAPMPKGAFYSVISLPIDDSDK
                     FCKWLLEEFSYENQTVMLAPASGFYATPGLGTKEARIAYVLKLEDLKGAVKCLEEALK
                     VYPGRIK"
     gene            24980..25912
                     /locus_tag="PKI35_05625"
     CDS             24980..25912
                     /locus_tag="PKI35_05625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX43898.1"
                     /translation="MKESFFLLTAIPSASIVVIQFGGYHSLIPAFLARLSHKPSVIVM
                     GGTDCVSFPSIGYGNLRKPLLRWFTLKSFRLASMLIPASQSLVEYDYDYCDKDYPKQG
                     YKYFDPGNKTPYKVIFNGISLNLFRPVEGIIREKNTFLTVCSNLDRRNYFIKGIDLFI
                     EAAKRFPGYKFVLVGKTASGFEIEKPENLRHIPFVSHDDLPGLMSSCSFYCQLSLSEG
                     FGVALTEAMACGCIPVVSKVGILDQIAGDSGFVLEKYDKDLLVKTIENAVNSDVVLLS
                     ERAGKRAAALFHDGRRFSELIKVISDLIEQNKRL"
     gene            26009..26986
                     /locus_tag="PKI35_05630"
     CDS             26009..26986
                     /locus_tag="PKI35_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HNX43899.1"
                     /translation="MNNQKKITIIGAGSIGTALGNVLSLRPDLNVNLLSVESDVVSDI
                     STLHINQKYFPNIRLNPSLKATTAPGVLRDSEIIFIGIPSTAVIPYLQEIRDELPENA
                     ILVNLAKGFGIHKKVIALCLRDHFPNPTCALKGPSFAREIINNVPTALTLASTSRDTI
                     QAVAPLFDGTTVYIDTTTDVAGVEVLSILKNIYAIIIGIVDAHFHSPNLRFFIFTKAF
                     NEMRNILLHVGGREETMFNYCGIGDFGLTALNDMSRNHTLGLLIGKGFFTEDISGKVV
                     LEGRIATEIFLEELEKSGLPPEKYPLMRELQQTFQGNHNVSDFVNKIIS"
     gene            complement(26998..28299)
                     /locus_tag="PKI35_05635"
     CDS             complement(26998..28299)
                     /locus_tag="PKI35_05635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX43900.1"
                     /translation="MKKALIITYYWPPSGGAGVQRWLKFTKHLRQFGWEPVIYTPENP
                     EIPAFDATLATDIPDNITVLKTPIWEPYTAYKKLVGMKQGDRINSGFLSEKKKSGIIE
                     SLSVWIRGNLFIPDARKFWIKPSVRYLTRYLSDHPADVIITTGPPHSVHLIGMELKRI
                     TGICWLADFRDPWTGIYYFDQLKLSRLAKRKHSGLEKKVLQQADGVTVVGNFMKEEFS
                     RIVKRNYCVIPNGFDEDDLEETGYGQADQKFSLSHFGTIYPLANPVLFWKVIAEKLAD
                     DSRFAEKLQIKLVGSADHTVMSAIQSNGLEKFVRKIGYLPHKEVIREMQKSQVLLLLV
                     NNTPQAKVILTGKLFEYLAVRRPVLCLGPDDGDAAALLKETNAGKNADYSDEISMRKI
                     LDEYFSLYLTGNLDAESRNTGQFSRRELTRRMAEVLNDLGS"
     gene            complement(28301..30799)
                     /locus_tag="PKI35_05640"
     CDS             complement(28301..30799)
                     /locus_tag="PKI35_05640"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNX43901.1"
                     /translation="MAQSNFLKKSYPYFLAILIFVALTLVYFSPLLEGKRMRQSDTMN
                     YQGMSKEISDYRAKTGEEALWTNSMFGGMPAYQISVVYKDNLVKYVDKIMKLGLPHPA
                     DLVFLYMIGFFILLLVLGVDVWLAVAGAVAFGFSSYFFIILEAGHNSKAHAIGYMAPV
                     LAGIILTTRGKYLLGGILSALFISLELYANHLQITYYLLMVVGIFMISELIFSIREKR
                     LPGFLKSIGVLIFASVIAVGCNITNLWSTYEYGKYTTRGKTELTTEAENRTTGLDKDY
                     ATDWSYGKAETFTLLVPNFMGGASQGKLSEKSESYKAMISNGVPENQADQVIKNMPLY
                     WGPQPGVAGPVYIGAIVIFLFVLGLFIVDNKYRWWLLGAALLSVLLAWGRNFMPLTDF
                     FLDYVPGYNKFRAVSMTLVIAEFVIPLLGVLALAKIFDGTIDKKQLNKKLLYALSITG
                     GLTLIFALFGGSLFNFSAKTDEQLKSVFPDWLMAAVQHDRASMLRSDALRSLLFIVLA
                     ASAIWACINGKLKKKYVFPALIALVVIDLWSVDRRYVNNDLFVRKSIAANPFQPTQAD
                     ELIMKDVDPYFRVFNQTVSPFNDASTSYFHKSIGGYHGAKLRRYQEIIDHHLSKGNMN
                     VFNMLNTKYFIMPDQNHQPMPQINFEALGNAWFVRDVKMVKNADEEINALNTFRPKET
                     AVVDKRFESFVKEHTFTFDSTATISLKSYSPNQLVYESSSPKEQLAVFSDIYYDKGWN
                     AYIDGKEAPYFRANYILRAMMVPAGKHTIEYKFHPGVYYTGEKISLASSSLLILAMIA
                     MFGWMIYKGRKNRHPETEINGPGITGNSKPGKSN"
     gene            complement(30829..31119)
                     /locus_tag="PKI35_05645"
     CDS             complement(30829..31119)
                     /locus_tag="PKI35_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43902.1"
                     /translation="MSQEFINEASLTGTMRVIIILIIIYACYSLLVRFIIPTVIKKSL
                     RNFQEKFYEENPDLRKEDNLKKEGEISIRRIDDRKARQLYNEAEDTEYEEIK"
     gene            complement(31293..31442)
                     /gene="rpmH"
                     /locus_tag="PKI35_05650"
     CDS             complement(31293..31442)
                     /gene="rpmH"
                     /locus_tag="PKI35_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNX43903.1"
                     /translation="MKRTFQPSIRKRKNKHGFRSRMETVGGRKVLAARRARGRKKLTV
                     SDEGK"
     gene            complement(31562..33022)
                     /locus_tag="PKI35_05655"
     CDS             complement(31562..33022)
                     /locus_tag="PKI35_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNX43904.1"
                     /translation="MNKEFNKLSPEEVWKNFVSLTQIPRPSKSEDRIQQFMVEFGKNL
                     GLETIHDEVGNVIIRKPATPGMENRKGVILQGHLDMVPQKNSDKDHDFEKDPIEAYID
                     GEWVTANGTTLGADNGMGVASAMAVLASKTLKHGPVEALFTCDEETGMTGAEGLKPGI
                     LKGEILLNLDSEEEGELYVGCAGGTNANAALKYREEPVPQGVVAYKLSLTGLKGGHSG
                     LEIILQRGNSNKLMNRFLYHAAEKHNLRLATIDGGSLRNAIPRESFAVVTIPADREES
                     FLKCAAKHHDLFRSELAAVEPNLKFTCEKTAMPASLIDADTFHKLMKAVYACPNGVIR
                     MSDDMPGLVETSTNLASVKMIDGEIRVQCLLRSSVDSSQDDLGNMIQSVFELAGASVE
                     LNGKYPGWKPNMNSPILNTMQNVYQKLYGKIPGIKAIHAGLECGILSGAYPHWDMISF
                     GPTLCYPHSPDEKVNIASVEKFWKFLTTTLENIPVK"
     gene            complement(33322..33942)
                     /gene="rsmG"
                     /locus_tag="PKI35_05660"
     CDS             complement(33322..33942)
                     /gene="rsmG"
                     /locus_tag="PKI35_05660"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNX43905.1"
                     /translation="MQLILKYFGDIPANSLQQLEALQGLYSEWNARINVISRKDMDNF
                     MERHVLHSLAIAKIIHFAAGTRILDVGTGGGFPGIPLAILFPESEFTLVDSIGKKIKV
                     VNEVKTAIGLKNVLAYQTRAEEIKNRYDFIVSRAVTTLPEFLGWVGNKIGPNNNNSLK
                     NGVLYLKGGDLQTELKDLKWRHGVFDLNTFFDEEFFVTKKIVHLYK"
     gene            34200..>34605
                     /locus_tag="PKI35_05665"
     CDS             34200..>34605
                     /locus_tag="PKI35_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HNX43906.1"
                     /translation="MEDIEWGQLPFGYMKTDINVRAYYKDGKWSKLEFSDSEYMPVHI
                     AATGLHYGQEAFEGLKAYMGKDGKIRLFRWEDNARRMQDSAKGLLMAVPPTELFREAI
                     IEAVKRNLRFVPPYGTGASLYIRPLLFGSGKKV"
BASE COUNT         8545 a         8026 c         8577 g         9457 t
ORIGIN      
        1 cgcccgcaag gcatcggcag ggttcaggat tcccttccgg atggacggca gcctgaagtc
       61 ctgcaccttc gtggggcagt atcctgaaat ggaccggaac ccgaagagta ccctgaaaat
      121 attgagcagg aaattgaaag gattccgcgt gaaaaaggca gaaacagggt aggagacaag
      181 cagtataaga gcagcaaaaa cgtcgaaaat ccgtttgctc cgccggtttt caagcttgcc
      241 gatcgagttg atatcgatca cgtaaaggtc gccggccgta ttgatggaat tgctgccgat
      301 gatggaaagg ctttcgggag gggcgatctt gtaatctacc tgcagatcct gcaattccga
      361 catgtggtcg atgatgactt cggcggcaag gtcctttgca cagaaaatga tttcgtcgat
      421 atcataaatg gtaatgatgt cgcggatctg tttgatgccg ccgaggaatc cgttcttttc
      481 accgttcagg tctccggcgc ggaccagtcc gataaaggcg ggtttataaa cggctttctg
      541 caacaggtcg ctcacccggt gggcttcgtc ggcatttcct acaatggcaa accggcggct
      601 tttggataca ttcagcacat atccgctgat tttgaacaga tgcagcaaca accggagtcc
      661 ggtcatcgcg atcagtcccc acatcgatcc gatcacgatc agcgcccttg aaaaccggaa
      721 ttgcagcggc aataacgcat aaaccaccag cgtaatcacc agtccggccc ctacgccgcg
      781 gacaacgcgc cccagtctta ccggtttgtc ataacctccg gagacgtaaa cggaaaggat
      841 gatcagcagg atataggccg gcagcacata gaaataaaac gcaggggcat aatgtccccc
      901 atagggatag atgacggtat gttcccagta tctcgtaatc agacaaatgc cggtataaat
      961 cagagcagta tcaagcaacg gcaacagcag cgtgcgcgcc gcgcggtgaa caacgctcag
     1021 cgaggccctg aagtatatgg ccatgttgat caggatggag aaaagccttg cgttcttacg
     1081 cgagaaatgt ttaccggcga agatgatcat cgcgttgtaa aacatgaaca cgtagttcag
     1141 gctgcctttt ttggtgcttt cgcctttgta atggatgatg cgcgtttcgg ggaagtagta
     1201 gtttttatac ccggccaggg tgatgcgata cgacaggtcg atgtcctccc cgtacataaa
     1261 gaactgttcg tcgagaaggc cggtcttttc gagcgtttcc cgccgcagca tcataaaggc
     1321 gcctgccagc acatccaccg gatttacctg gccgtcgtcg aggtaaccca ggtgataacg
     1381 gttaaaagtc ctggaacgcg ggaatagccg ggccagcccg aatatcttat aaaaggcgac
     1441 catgggtttc ggtaatcccc gtttcgattc aggaagaaaa tggccggttc cgtcaaccat
     1501 ttttaccccc agcccgccgg catcagggtg ttcttccata aaagcaaccg ttttccggaa
     1561 agtgtccgat tcaacgacgg tgtcgggatt gagcagcagg atatagtcgc ccgtcgactg
     1621 cctgatcgcc tggttattgg ctctggaaaa accgacgttt tcggtatttg cgatgcaatg
     1681 cacctccggg aacttttctt tcagcatctt gacggaacca tccaccgacg cattatccac
     1741 gacaaagatt tccatctcca tgtcctgccc ggccgtaatg accgagtgca ggcattgttc
     1801 gaggaaatac ctgacgttgt aacttactat gacgaccgat aatttcaaaa acggatcttt
     1861 cggtggttgc ttgctgaatc aggcgcaaat atcggaaaaa tgggccatag tgttttaagt
     1921 tttgtttgcg gaattgaaga tccggggatt cgtggtcctg agagggataa cggatcagtg
     1981 aataccatcg gccccgtggc aacagcgatc agatatccgg cttttcctga gcgtttccgg
     2041 ttcaggcggc ctgcatcagc cgttccggcg gggaaaaata gtccgtcagg attcctgttc
     2101 cggaattcag ctattatatc gcaggtcaga cagttatgta aatattacgg gctgtcggga
     2161 gaggccgttt ccggcgcgaa aaggcaatcg aagacaacgg cttcatggtt tttattatat
     2221 tgtccctaac tttgcagcgc cggggctgat aaccgggtat cctgccggaa gccggcgccc
     2281 gggtttgtgt tgaccgtcgc tgccggctga caatcagtag attaaaatta actgctttta
     2341 tataaccgaa ggatgatccc gatagctgcc ttttttgcga ttatactggt cacgttgata
     2401 ttgatccctt ttttaaaggt gcgcctgaag gggattataa ctttcctggc cgtggtgctg
     2461 aatgcggcaa taaccgggta ttatgcggta ctgagccttt ccggccagac ctttttatat
     2521 gaattgcccg gcagcctttt cacgggaagt attccgctgc gcctcgatgc cctctcgggc
     2581 tggttcatcc tgattatcaa tttcgtattc cttaccggcg gcttttacgg tttcttctac
     2641 ctgaaggctt acaaatcgca ggttcacaac ctggggcttc atgccatttg ttttttgata
     2701 cagcatgcat cccttctcag cgcggtcatt gtgcagaaca gcattgcctt tttaattgcc
     2761 tgggaaatgc tggcgctgac atcgttcctg ctgatcatct ttgagcgtga aaaagagacg
     2821 accattgcgg ccgggctgaa ttacctggtg caatcgcact tctccttcat ctttctggtg
     2881 attggtttca tctgggtagc gcaaaaaaca gggagctatg attttaacgc cgttacggct
     2941 tacgcctctt ctttgcctgt tggggcttcc atgctcctgt tcatgatttt tttcaccggt
     3001 ttcgctttca agtccggctt tgtgcctttt catacctggc tgcctcacgc acatccggcc
     3061 gccccttcgc atgtgtcggg catgatgtcg ggcgtggtga tcaaagcggg gatatacggc
     3121 attctcagga tgctgctgct gataaaaacg gattatacca cgatcgggta catcatcctg
     3181 ggcgtttcgg tgatatccgg tttatacggc gtgatgctgg ccattgttca acatgacctg
     3241 aaaaaactgc tggcctacca cagcatcgaa aatatcggca tcatcggatt gggaatcggg
     3301 gttgggtgca tcgggatggg agcgggcaac tatctggtgg ccatgcttgg ctttgcgggg
     3361 gctttgctgc acaccctgaa ccatgccctt ttcaaatccc tgctgtttta tgccgccggt
     3421 atcgtttacc aggcgacgca caccctgaat atcgaacaac tgggagggct gatcaaaaag
     3481 atgccgcaaa cggcagccct gtttttaatt gccgccatcg ccatttcagg gataccgcca
     3541 ttcaacggct ttatttcgga gtttctcatc tattccggat tatacaactg gcttcagggg
     3601 gcgtcgctgg catcgctgat ttgctgcctt ttcatgatca tggggctggt gctgatcggc
     3661 ggactggccc tgctttgctt caccaaagct ttcgggatcg tcttcctggg gaatccgagg
     3721 catgcgttcc atcacgaaat aaaagaagcc ccgtttatgc aattgctgcc cctttatctc
     3781 atcgcttttc tgatcgtttc catcggattg ttccccgggt acttcctgaa tatgatcatg
     3841 gcgccggtga atctgttccc gggcattcag ggcgttgccg gaactctttt ccgggagaat
     3901 accgccggta tcatgcagaa catcagttgg gccatgtggg gcttgatact gcttacttcg
     3961 ttgatatacc tggtcaggaa actggcgatg cagaaaaggg aagttgccgc ggattcaacc
     4021 tggggatgcg ggtatgtggc cccgacggca aaacagcagt acacggcagg ctctttcgta
     4081 aagacataca gtcaattgaa ccgggcgacg ttatccatta agaaacatga aaaaagggta
     4141 gacgggctgt atcccggaga agcgcattat gaaacccatg cctatgacca gttggagaaa
     4201 tggctgattg ataaaccttt ggatggtatc aggacgtttt taggctggtt tcggtttttc
     4261 cagaacggca ggctgcagtc ctatattctg tatggcattg tctttatcct ggcgctgatc
     4321 tttctgccca tgatttttga ctcgttatcc ggctttattg acttcctaaa acatctttaa
     4381 acatgctgag ccttgtattg atattaattg cggcggtatt tttcaccggg atcatcaatc
     4441 gcacgaaaag catcttttcg gggcggaaag gccccggcat cttacagccg gttaaggatg
     4501 tgatccggtt gttccggaaa ggggccgttt acagccggac taccagcttt atctttcaga
     4561 tggctcccgc cgtttatctg tcgtcggtgg tgatggcgat gctcgtggtt ccgttcggac
     4621 aatccaaagg ggtgatcagt ttcagcggtg actttatatt ctttgcctat gtgctggcgc
     4681 tgggtaaatt tttcaacatc attgcggcgc tggataccgg cagcagcttc gaagggatgg
     4741 gggcgagccg cgaagcgctt ttctcgatgt ttgccgagcc ggctttcttt atcctgatcg
     4801 gttcgctggc tttactcaca ggatatacgt ccttttacga aatttttgca gccttccatg
     4861 tggggtcgcc cgtcagctat gccctggcag tactggcggc ttttgtgctg atcatgatca
     4921 ccatgatcga aaacagccgg atgccgattg acgatcccaa aacgcacctt gagctgacca
     4981 tgatccatga agtgatgatc ctggattaca gcggtttcga cctggggctc atttttactg
     5041 ccggactgtt gaaatttgcc atctatgggg cgcttatcgt gaacctgttc attgggatat
     5101 tcccgtacga atattccatc ccgatgttct ttgccatcca gtttgtgacg gccgtattga
     5161 tcggggctgt tgaatcgttc atggccaggt tcaggatgaa tcacaacgcc cagtttatct
     5221 tcgcgctcac ttcggtaagc ctcctgatct tcctgggagc attgctgatc ctgggtaagt
     5281 ttgtttaagc cttaaaacac gaaaagatga ttaatgtatt gctgataaca ttcctgatca
     5341 ccctgtttta cgtggccatt gccaaccgcc tgctgacgta tgtaaaagtg ctggcattcc
     5401 agggcgtcct gcttttcgtg gtttcgttta ttcaacttag cgagatcaat acgctgaacc
     5461 tggtgctgat cctgatggaa acggtctttt ttaagtcggt ggcggtgccc ctgtttttgg
     5521 cctacgtcct gaaaagaaat aagatcacgc gtgaagccga accttacgta tccaacttca
     5581 tctcgctgat catagtcacc tttatctgtg tgatttccat cctgcttgtc aacagcatac
     5641 aggacactga atcggataaa atcttctatg tggtggcgct ggctgccctg ttcacgggga
     5701 tttattttat catttcccgc cgcaagatca ttacacacgt aatgggctat cttattgttg
     5761 aaaacggtgt ttttatcctt tcgctggcgg taggggacaa aatgaccatg ctggtcaaca
     5821 tcgggatcat gctcgatatt tttgtcagcg tgctggtgct gggaatattt gccaacagga
     5881 tcggcgatac tttcgaggcg atgaatgtgg ataacctgac caacttaaaa gactgatttg
     5941 tatgtttcaa tattatctga tattggtcct tgtacttgcc gccggcgtgt ttttcaaccg
     6001 gaaccgtgcc gtgcgcggcg tgttgcttgg aatttttctc ctgcttcaga catccgttac
     6061 catctatggt tattatcata tcggcgggca ggaagctgac ggttatttca gctttgatgc
     6121 gctcgggctc atctttcttt cggtgctgac gatcctgagt tttaccacca tccttcacag
     6181 cctggtttac ctgaaacaca gggaggacga ctggcgccgt cagcgaatct attttaccgc
     6241 ccttatcctc ctgatcggcg ctatgacaag cgtgtacctg gcttctgcca tcggcatcat
     6301 gtgggtgctc atcgagatga cgaccctgag tgtggcagcg cttatctatc acgaacggac
     6361 ccacttatcg ctcgaagcaa cctggaagta cgtatttatc tgttccatcg gcatcacatt
     6421 tgcttttgta ggtattgttt tcctcagcaa ggcattgcag gaaggcggct cggtaaatct
     6481 cgccttcagc gatataccgt tgcatctgca gaatgccggc gctttctggc tgaaactggc
     6541 ttttattttc atggttgtcg gcttcagcac caagatggga ctcttcccga tgcacaccat
     6601 caccgtggac gcccacacgg ttgctccgcc accgatcagc gcctttatct caaccacgct
     6661 gatgaatgtg ggttttgtgg ccattttccg cacttatacg ctgatgtcgc atacctgcat
     6721 cctgccctgg atgaaccagg tattgctatg gagcggcatc ctttccgtct ttatttcgac
     6781 ggtgtatatc ctcaaggcag accatataaa aaggatgttt gcatactcca gcctcgaaca
     6841 tatgggattg tcggcgctgg ggctggcagc cgggggcatc ggctatttcg cagccatctt
     6901 gcacatggtg cttcattcgt ttatcaaggc cagcgtcttt taccagatcg accaggtgca
     6961 ccggactttc ggcacctatg tgatcagtaa aaccgggaat tacttcaata aaaacctggc
     7021 cggggcgctg gtgatgctgt ttgtatatat cagcattacg gccattccgc cttcaggaat
     7081 gttcgtaagc gagttgctgg tattccggtc gctgttcgaa tccaaccata tcatcgtcct
     7141 cattctcatt ctgctgcttc tcaccatgat catctggtcc atggggaaga atatttttaa
     7201 aatgcttttt gttcccggta aggatgctga tgcgacgata aaagcggata ttaaaaagtg
     7261 ggaatcggtg agccagttta ttctgcttgc tttgggcgta tatctcggat tgaacccgcc
     7321 ttccggtttt gtgcaactca tccgggatgc tgtgatgctg ttaccccagt gatatgaaat
     7381 atataaccat ccataataac caggctgttc cggtttccga aattccggaa agcgactatg
     7441 aggcttttct ggagttgaat gccggttttg ttcagcaatc cgttgaaagg cactgcgtca
     7501 gctatttcgg atatgtttcc ggggcaggag taaaacttgt ttgttgcatc gccgatgatc
     7561 agagtcacga aatctatgtt tcttcatcgc tggtgcaaac ggataagaaa ttgccttcct
     7621 tcacggcccg gcatacatgt tttgaaaagt ttgaacgcga acttcacgaa aacttcggga
     7681 tcagctattc ggaccatccg tggatgaagc ctgtgaggta tgcccacgat cgttatgaca
     7741 agggagatac tatcggggat tacccgtttt attccatcga aagcgaagaa cttcacgaag
     7801 taggggtggg gcctatccat gccggggtga tcgaaccggg gcatttcagg ttcatctgca
     7861 acggggagaa gatattgcat cttgaaatcc agttgggata ccaacatcgc ggaatcgaga
     7921 aactgtttgt tgagaagaag ccgcttgccc agttggcaac cctggctgag aatattgccg
     7981 gcgatactgc tgtcggccat tcggtggctt tcgcgcaact gtgggaatgc ctgtcgggtt
     8041 tcgaaacatc gcgggatgtt gattatgcac ggaccatcgc gcttgagctg gaacgaatcg
     8101 ccatccatac cggcgacctg agcgccgtat gtaccgacat tgcataccag cttggcagtt
     8161 cggtattcgg caggctgaga acccccatca tcaactttat gcaggaatgg tgcggtaacc
     8221 gtttgtcgaa agggctaatc aggcctggca gaaataaata tccgtttacg caggcgcttg
     8281 ccggcaggct gacggaagtg ctcgatcgct tcgaacctga ttttatcgaa atgaacaggg
     8341 aattgttcct gcttccaagt gcattgtcgc gttttgagcg caccggagtt gtaacggccg
     8401 aggatgcttc agccatcgga gcggttggga tggcagcgcg cgcatccggt ctcagccgcg
     8461 atatccgtaa atcgcacccg catgccctct atcctgaact tggccatgaa cccgtggtga
     8521 agcgtcacgg agatgtgtac tcgcgtgtgc aggtgcgaaa ggaagaagtc tatcagtcgg
     8581 ttgcgtacct gcggaagttg ctggaagata ttcccggtaa tgctgaaaag gaaacgcttc
     8641 agtcgcccgg gagggattta ttcgctcttt cacttgttga aggctggcgc ggcgaaatat
     8701 gtcattgtgc cgttaccggg cccgatggca gtctgaaagt ctataaagtc aaggatccgt
     8761 cgttccacaa ctggatggcc ctggcccttg ccgtgcggaa caacgaaata tccgatttcc
     8821 cgatctgcaa caagagtttt aacctgtcgt actgcgggca cgacctctga tcagtaaacg
     8881 aaattctatg ctgaacaacc taaagatatt attccaccag ggcaaacaat atatcccgga
     8941 cgtcaccacg gccaaagtcc ccggcatatt caggggacga ccggtaataa gcccggagcc
     9001 ggcggacgga caaagacttg cggatgtttg tcccacaggg gctatttctg taaatccggt
     9061 gtgcatcgac cttgggaagt gtactttttg cggtgaatgc gccctgatgt ttccggagaa
     9121 aatacggttt accaccgatt ataaaatctc tgtaacggaa cgggatcaac tggttgtgaa
     9181 ggaaggagct gaccagcctc tggtgatcga tccggataag gttagaaaag agataaggaa
     9241 gattttcggc cattcgctga aattcaggca ggtatcggcc ggtggcgaca acagttgtga
     9301 atgggaactg aatgcctgcg gcaatgtaaa ttttgatatg gggcgtttcg ggattgagtt
     9361 tgtggcatct ccccggcatg ccgacgggat tgttattacc ggccccatca ctgaaaatat
     9421 ggcggagcct ttacagatct gctatgatgc cgttcctgct ccaaagatca tcatccttgc
     9481 cggtaccgat gccatcagcg gcggcatatt tgccggcagc cctgccctcg accggcgttt
     9541 catcgaaaaa tatcctatcg atctgtttgt ccccggcaac ccggtgcatc cgctttcttt
     9601 tatcaacggc gtgctctccc tgatgggaag gaagcctccg gtgaaaccgc agtaacagcc
     9661 ttctgaatca tcgccgccgg atatcatccg aaaataccgc atccttaaca aactccttgt
     9721 ccggagtgag aagggcctcc ttttgctgat atctcccttt atgataatca ggaattacag
     9781 cgcaggggaa gagaaattca ggaattcaga tactgcccca aggcagggcg atgggtcgca
     9841 gaaggtctgc cggtcagctt acatctgaaa tttcctttat atcagtgcat cccgttgaca
     9901 ccggttccga aaagggcata atcatatttc acgggatcgc cggcgttaaa aagccggagg
     9961 ttggccgtga gttcttcaac ggctttccag tcgtcctgtt tccgtttcag aagtccgagc
    10021 attcgtgcaa tacgtcccga atgaacatcg agcgggcaca tgaggtgatc gggattaatc
    10081 ctcttccaga ggccgaaatc aacgccccgg ccgtcgtttc gtaccatcca ccgcaggaac
    10141 atatttaccc gcttggcggc cgaaccggaa cctggatcag caagatgctt tgccgaacga
    10201 accagatggg gcatttcaag aaatttcttc ctgaaggtga tgattccgtc ccggacggag
    10261 ttttcctttc tgtatccttc ggtaaacagt tgctccatgg tttcatattc acggtaaacg
    10321 gaacgtaatg aatacatgaa gaagaggcag tcgtcgccat tgaaggtgcg gtaaatgaaa
    10381 cggctaaaac gttcaaattc tttttccggg gcctgcgtca ggaaattata cggttcgtta
    10441 tccatcagtt ccgtaagggc tctggctttg gcaatgatct ggggcctttg cccccacgat
    10501 aaagtggccg aaaggaaacc ggcaatttcg atgtccgggg gtgaattaaa acgatgggga
    10561 acggaaaccg ggtcgcgcgc tatgaaatcc gcggtattga attcgtcgga tttcgcatcg
    10621 agaaaggctt tcaggttccg catttccggg ttcatgagcg atgtatgata aatacaaaac
    10681 tcccttcgac cgtttccgga ggaagggagt caaaaagaag taaggattag tttagaatag
    10741 taacggcatt gaccagatct ttcccgagtt cgatggcttt ctcgttttcg gggatcatgt
    10801 tatgatgcct tgccggcaga gatttttcga gtcctttgtg aataaattcc atgctcacga
    10861 tgggtttgag tttcaggaaa ccgcccagca caatcatatt gaaaaccttg ctcatgccaa
    10921 gtgctccggc cttttcggca gcctcaatgg tatatacatt gatgtccttt ctctcgggat
    10981 gcctggtaat tccgttggga tcgtaaatga gtaatccgcc tggttttacc gtcgattcga
    11041 atttgtccat cgattgctgg ttgaggatga tagccgtatc gtaagcgttc aggatcggtg
    11101 aactgattct ttcgtcgctg ataataacgg taacgttggc tgtgcctcca cgcatttcag
    11161 ggccgtagga tggcatccag ctcacctctt tattttgcat aactccggaa tatgccagaa
    11221 tttttcccat ggaaaggaca ccttgtccac caaatccggc aataatgatt tcttccgtca
    11281 tgatattgtt ttttagcgtt aacaatgaaa tgtgaaggtc gtcccttctt attcgttcac
    11341 tttgatatct cccagcgggt agaaggggaa catgttttca accatccagt cgttggcctg
    11401 acgcggggtc attttccaac cggaattgca gtttgaaacg atttcaacga aggacaatcc
    11461 cttgttgagt ttcgtgtttt cgaaagcttt ccggatagcc tttttcgtcc tgcggacggc
    11521 ggcgggtgta tggacagcct ggcgggtaac atataatgtt ccgggaagct gggcgaccag
    11581 ttcggtgatt ttcaggggat ttcccatcat cgctatatca cggccgtagg ggcatgtagc
    11641 ggttttcatt ccttcgagcg tggttggggc catctgacca ccggtcatgc cgtaaatacc
    11701 gttgttgatg aaaatgatca cgatattttc accgcggttg caggcgtgaa tggtttcagc
    11761 ggtaccaatg gctgcaaggt cgccgtcgcc ctggtaggta aatacgaatt taccggggtt
    11821 cagcctctta acggcggttg ccagggcagg tgcgcggccg tgggctgctt cctgcatatc
    11881 aatgtccatg taatcgtaag caagcaccga gcatcctacg ggtgcgatac cgattgtttg
    11941 ggactgaata cccatttctt cgatcacttc catgatgatg cggtgggttg tgccatgtcc
    12001 acagcccggg caatagtgaa gcgttctcgg agtaagcaat tccgtgcgtt tgtatacgag
    12061 attctcaggt tttacaatct cagcattcat aattacttct gccatgactt atcctccaat
    12121 aatttgtttt ttcagggctg ccactacttc atcaggtgaa gggatgattc cgccatacct
    12181 gccgaaatat tcaactttaa cctggcattc aacggcaagc cttacatctt ccaccatctg
    12241 tccggcgctc atttcaacaa ccagcatgcc tttggttttc ttagccagct tgttgaggat
    12301 ttttttcggg aacgggaaca gggtgatggg ccttaataaa ccgactttga tgccttcggc
    12361 gcgggccagt tggatagctt tctggcaaat acgggcgctg gttccgtagg caacgaaaat
    12421 gtactcggca tcgtcgcaac tgatttcttc ataccgtact tcctgttctt ccataagcct
    12481 gtatttagcc tgtaaatcaa gattatgttt ctccatcctg gtggattcga ggtcgaggga
    12541 agttacgatg ttatgttcgc ggtcagaagt tttgcctgtg gtagcccagg gcgtgttctt
    12601 ttttatttct tcatcggtga aacggggcct ttgcgggtcg agttccactt tttccatcat
    12661 ctggccgatg gcgccgtcgg aaagtacaag aaccgggtta cggtatttga aagccaagtc
    12721 gaaaccaagg cgtgcaaagt cggccatttc ctgtaccgat gacggggcca gtaccagcaa
    12781 cctgtagtcg ccgtgtccgc cgccttttgt actctggaaa taatcggatt gcgaaggctg
    12841 gatggttcct aatccggggc ctccgcgtac ggcgttcagg atcaggcagg gcagttcggc
    12901 gccggcaata tagctgatgc cttcctgttt gaggctgatg ccggggctcg aagacgaggt
    12961 cataacacgt ttaccggtag cagcgccgcc gtaaaccata ttgatggctg caatttcgct
    13021 ttcggcctgt aaaactatca tcccggtacg ttcatgaggt ttctgggcca taaggtattc
    13081 catcacctcc gactggggag ttatcgggta accgaaatag ccatcggcgc cggcacggat
    13141 agccgcttcg gcaaaagctt cgttaccctt cattaattta agttctctca tgctttcgca
    13201 atattttgaa taaattattc gacttttttt ctgtaaacag tgatggcacc gtcggggcat
    13261 accactgcac agttcgtgca accggtgcat gcatccttta caggctgtgc ataaaaatac
    13321 cctttgtggt ttacatgttt cgacattgct attacttctt cgggacaggc cgggatacat
    13381 acttcgcagc ctttacagcg ctcggtatcc acaacgattt ggcctttcaa ttttgccata
    13441 gtactacgtg tttcacttaa attttatgcg aaattagata tttaaagcaa acggaaacca
    13501 aaagtcagta tttattgctg gcatgtctgc caatttaaaa ggattttaaa tataaaatcc
    13561 tgccggctca gggtccccgg atttgctgtt tacgctaccg gatgatgctc tttgaatttc
    13621 ttcagacgca tctccaggtc ggggaactgc gaccggctta ccagcgacac gcaggcccgt
    13681 agcccttcgt gacgctcact gccggtgatg gacaatgaaa tggcgctgat accataatac
    13741 aggagttctt cgatgagttc gggcccgtcg aaaccgggat acatgatggt gaaataaaat
    13801 ccatcggcaa ccggctgatc aaggtcctta tcgtaaacga tacggaaacc gttatcggtg
    13861 aacagcttct tcatgatgtt tgccttacgg ccgtattccc tgacatcttc cacaaaattg
    13921 aaccgtcctt cgttggcggc tttcagcata gcggcaaggg cgtattgagc ggagtgggca
    13981 gtacccgaac tcagcgcata tacagttccg aaaatcattg ctttcccgaa aatgtcgctg
    14041 ctgtaatatc tgagaaggtc aggagctttg gtatggaaca acttgtcgct gatgaccatc
    14101 agcccgatac gctgtccggc ataactgaag gcttttgaac tcgagataaa caggatgtag
    14161 ttatcggtgt atttggccac ggtcggctgg aacggcggca ccccgggctg gctgtaatct
    14221 ttcctgaaat ccatggcgaa ataggccagg tcttcaagga tcacaacatt atatttgttg
    14281 gccagatcgc cgatgatctt cagctcttca tccgtgaagc atatccagga gggattgttg
    14341 ggattggaat acagtacgga atgaatattt cctttgctga aataactttc gagtttggtt
    14401 ctcagcgctt ctccgcgata tccatataca tcaaaacctt caaatttttg tcccagtact
    14461 ttatgctgtt gtttgtggac cgggaaactg ggatcgatga acagcgtggt gtcgcgttcc
    14521 ttatgcatcc gggccagtgt aaggaaggag gcaaacccgc cctgcatcga gcctactgta
    14581 gggatgcagc catcagggct gacctcaaca tccagaaaat tcttaacgaa ccgggaagct
    14641 tccgttttca attcagggat gccgtcaatg tcgggataga tggcagcaac gccatgcctc
    14701 agcgcatcga tttccgcttc ggttccgata gcggcgggcg ggaggccggg aacgcccatt
    14761 tccatccgga tgaattttat gccgctggcg cgttcaatgt tgtcaatgag cctttttatt
    14821 tccctgatcg tggctttgcc aacgacaggg acattcagct ccgagatttt ttcatttacg
    14881 atgtcaccgt tgataggtgt gtcttttgcg gttgttttca tttgtatatc tgcatgttat
    14941 atttctaaat ctgattgata agagttacag caaagttgga aaatagatac cagataagga
    15001 aataaactca caaatttatt atccggccag gagaatttag aatgagttta gtttatcccg
    15061 gtcaggataa ggcaaagcag gtattgttaa ttttcggaga tcatgttctg aaaggttttt
    15121 cagcagcatg atcagcggaa taccgttccg gtccttcagc ctgatattta ccggggttat
    15181 tttttagcgc ctttcatccc ggcaaatgca agcagcaatc caagggctat gcttaatata
    15241 gcggcaaaca gcacctggaa atcgggcggg agaaggaacg tgatcgtatg ggccggaatc
    15301 cagaaaaagg ggatcgtttt tttgaaaaca aaattccact gaacgtccca gtttatattc
    15361 ctgaatattt cagcaaaagg gatcggcctg aagagtccct gcagggttcc tcctgtctgg
    15421 atgatatgtg catcggtgat tttatgaaag gtcatcatca ccggggcata cgtaagattc
    15481 atgcccgccg aaacacaaaa agcaaccagt attttcatca ggctcaggtc tccctgcatc
    15541 acctgaacgg catcgttgag tcccaggtac tggacaaaaa gaggcatgcc ggcggcgaat
    15601 acaacaaagg ccgctttgat ggttaatccc aggaagcccc aaaccatggc gcggggaatg
    15661 attccaaatc ccggttcgtg ataaactccc tttctgatcc gcagggcgat catttcaccc
    15721 agggtcgcaa gcacggcaaa tttgatgaag ctcatgatca ttccatgatg gctgttgaag
    15781 cttacatagg ttgagtaaac gctgtcgaag atgaaaaagg ggagcagcag gataatgaac
    15841 agggtgataa aaaccaggtc tttgcgtttc acggcgggca atttcgtttt tttgcaaaga
    15901 taaaatgata gcgtggaaag aaaaatcata tatttacaat caaaatgttt ttatatgcga
    15961 ttcttttttc tgttttcatt tatctttctg caaatagcag ttcaggctca aatcaatacc
    16021 cgggtaaaga gtaccaaaga tcaggatagc ctggcccgaa caaatagtat caccgatacg
    16081 accagtttca ctcctgaaag ccggaacccg gataatcctt ttattgatta taagccggca
    16141 gtgatatatc tgacgtcttc tgattctatt gaagatcatg cttatatccg gcagaacttt
    16201 accgatcaga tggtttccgt gtggtcgaaa cccaaccggc tttaccctgc cagcaaaata
    16261 tacgggctga ggatggacgg aaaattattc agggccgtaa ggaaaagcgc ttatgaatgt
    16321 gtatttgccg aaagggcagt gcatgggccg atgagccttt atatctatac ccggataccg
    16381 cagtcttcag gatgggttga gtgctattcc gccggagggt ataccaacaa catgattgtt
    16441 gaaaacaagg tgacccggaa taaaaattcg tatggctatt ttgtcagtct gtatccggat
    16501 acgaataatt ttatcgctgc cgatgatatc aacaaatttg ccgagtccta tttaaaagat
    16561 atgccggaaa cgtataaaca ggcgtcgccg tttctgaaaa taaagcgtta caaggcgcag
    16621 aagcaaatcc tgacccttgc tatgctcgtg ggagtaggag gtgtggcgtt tatcgacagc
    16681 aatatgaaat ggctcttcct tgccggtttc ccggttgccg ctatcctgac ttttattaac
    16741 aggtctcata tccccgactg gaaggatatg gttgccattg tcaacagcta taacagggaa
    16801 acactcaccg ccgcagggca gtaaagccaa caccggtaaa ttgatctgct ttcagcatgc
    16861 ctgtttcccg aacaatccgg ccgggaatat tctatgcgtt tttctcaatc ctgatcctgg
    16921 catcaacggc aacaatgata tcttctttgc caagcaacgg gttaaggtca agttccatga
    16981 tttccggggc agcctgcagt aaagccgaaa gccttaccac aacatcggca aatcgcggaa
    17041 tgtcaacgcc ttgttgcccg cgcacacctt cgaggatttt aatgccccgg agccttctga
    17101 tcatggattc ggcttcatca tgcgataagg gtgaaagacc ggccgaaaca tctttcagca
    17161 cctcgatgaa gatgccgccc agcccgcaaa gcaccatgtg gccgaagcct ggttcgtact
    17221 ttgcgccggc aaataattca attcccgagt gcatggattg aagcaggatt gccgtagtgt
    17281 ctttgatcct gatcatccgg tcaaattctg cggctacctg atcgcaattg ttcacattga
    17341 ggactacgcc cccgacatca gatttgtgaa tcggtccgac aaccttcatt acaacaggaa
    17401 atcccaggac tgttgcagct ttgacagcct cgtcctttga ggtgacaaca gcttcgccgg
    17461 ccctccggat gcctgccgag tcgagcaact gctggatttc gccgggagaa aggtatccgt
    17521 tcgcggcatt atcgatgact tccctgacag tcttaatgtc aacggcgggg agttcaattt
    17581 tttcaggctg tggtttcgga gtatggtaaa ccttacataa tgcttttccg aagatcacct
    17641 cttcagggaa attgatcctg ccgagggaaa tgaaataatc gatctgttgt tttacattta
    17701 caatcgaagg cagaatcggg aaaatgggct tgttacaggt atgcattttc tcgtcaagta
    17761 ccttataaac atccgaaacg tcaaacaacc ccgggctgcc gaaaatcaca gccatcccgt
    17821 cgatgttatc aaatttttta tccacatgct cgatgatttc accgagttgt tctgctgttc
    17881 cggtagccag aaagtcaatg ggattggcaa ccgatgatcc ggcaaagagt ttggaaagca
    17941 gttcgggact ttcgagatgg ggaatttcca gcccgccttc cgataaggca tcagtcagca
    18001 tcacggcagg gccgccggcg tgggttataa tggctatgtt tttgcctttc agttctttat
    18061 gcataaatat ccctgccacg gtggtgagct cttcacgtcc gtaacaacgt actatcccgg
    18121 ccttgcggaa aagggcatcc acagctacgt cgggacttgc catagcgccg gtgtgagaag
    18181 atgcggcgcg gcttccggca gcagaacttc cagctttgat ggctgcaata cggcatcctt
    18241 tacgtatcag cgatgaggca tgtttcagca acatggcggg ccggttcacg ctttcgatgt
    18301 aaagcaattt tacccgagaa ctcttttcag gatcaaaggt ttcgtcaagg tgcattaaaa
    18361 catcttcaac tcccatctgg gcgctgtttc ccactgaata aaccgaggcg aaagaaagcc
    18421 ccttctgcat ggctgtctcc atgatgaaaa cggccgtggc gcccgatccc gatataaagt
    18481 caatgccttt gggattaaat ttcgggatag gaagcgaaaa aacagaagta tgatttgaat
    18541 tcatcattcc gatgcagttt ggccctatca gcgctgcgcc gtaccggttg caggtttcaa
    18601 caattttctt ttccagtttt gccccttcgg cgctttcttc cgagaaaccg gctgaaaaaa
    18661 tcacgaaggc gcgtgtattc ttttcacggg caagcgtttc aacggtatca gggcagaagc
    18721 gggctgcaat ggccaggatg gccaggtcag cctggggaat atcggctgca tccttgtaac
    18781 atgtcaatcc ctgtatattt gtttccttgg ggtttactgc atataacttt cctggataat
    18841 gatggtccag aaggttcttc agcgcttttc ccccggtttt tgtgacgtcg ttggatgcgc
    18901 ctattactac tatactttga ggattaatta gttgagatgt gatcatagtc ggcaaatgga
    18961 taaatgattt tttttgcgaa ccctgaaaaa tcaaggttgc cgggcacggg ttgtgccctc
    19021 aaactgtcgg cgaagatagg aaatttgctc cagattaagt ttagaaatct gtccggccgg
    19081 ccggtctgga tgtcgcctgc aaaccattca taggacgctc tacaacgtac cggttcataa
    19141 tgaaaaggac agaataaccg gggatcagcc ggtttgcaga atcatcatct ccgggttgtt
    19201 gaggaagatc gccggtattt tgggaatatg ctgacggcat ccgacggctc ccgatattgc
    19261 atcgggtgga ggatagcccg aaacataacg aggataagcc atttttacca tccaataaat
    19321 attaatctgt ccggcaacta aatcgacaag ataattgcgt tgcatctcaa atagttattg
    19381 ttatttttca gctttctgca ttagcaacag aaagataatg agcttgatag acatgaataa
    19441 ttcacaagcc gtttaaacgc ggattttgat ctgtttttgt gcccggagag catatcacga
    19501 ataaaagcca ggttgattaa tattcagaag ccaatcttca gcaagcatga aaatccggag
    19561 agaccaacct atgatatgcc ttatatgtga cggcagggga tacctgatca tcgatagcaa
    19621 gagctgtacc tgtaagtatt gtaaagggaa aggcgtatta gcatcaggcc aatcctgata
    19681 tcgggcaata ccgctttcgg gttttggctt ttcaggaaac tatgaaaatg ccggttttgc
    19741 tcttctttga tcttaattat tgccttttat tgacgaggtc gcccgatact ttgtcggaat
    19801 atcggatttt tgtcacggga atgtaaaatg cccgcagcgc gtcaacataa aggctaaaaa
    19861 gccggataaa cttctcagga attcaggtca gaaggagtga tggcgcctga ccttaaaaaa
    19921 ccggcaggag gatattgccc ttgtgagatt tctgcacgtt tctgcaagac tattgcatca
    19981 ggatggagac aaccgttgtt cccggccctc cgttcacgcc caacacaggg ctgacatcgt
    20041 tgataaaacg cggtttcatt cctgtaagcg tttccatttg ctgagcatac cagtcggcag
    20101 ttgcctggtt atggacgtgt gtgatggcat atccccaggc ttttccctct ttgaggagtc
    20161 cttcgatctg tcgcatgata atccgcatgc ttcctttttc attaaaggct tttccgaaag
    20221 tagtggcctt tccatcttca tccattgaga tgatggggtt gatgcccaac agtttgccaa
    20281 tcattccttt agaatagctt agacgtccgc tgcgcaccag atacttcact gttttggcgc
    20341 ttaccaggat gtggtttttt ttcttccagg tttcgatctg gtcgaccaga tcatcgtgat
    20401 tcgcaccttt ctctatagct tcggcagccc tcagcacgac aaggccgagc gcaccggtca
    20461 gcctgtttga cgataatacc gaaattttct tacccgactg ttcgcttaca gcttttgcgg
    20521 ccctttcgct gtttgagaac gttccgctga gatggcggct gatgtgaatg cctatgattg
    20581 aatcgtaatg tgtggcgagg aaagaatact tattgatgaa ttccttgtat gccggttggg
    20641 cggttgttgg cagaaccggc gattcttcaa aaagtttata aaactgttct gatgatatgg
    20701 ttgtgcgatc caggaaatgg ctttcgccga agtggaggct gaggggggcc accgagatat
    20761 ggtatttctc aaggatatcc tgtggcaggt cgcaggttga gtcggtaagc agggctaccg
    20821 gccatttccg gttatgcgac atttcgcgct gcatcaccat atcatcgact ttctggtagg
    20881 tgatacaggc ttttttgccc aggattccga aaagcgaaat gggatcgtcg gaatgaatat
    20941 gcactctcag cttacggtca gaaccggcaa ctaccagcga atcgccaagg tgtgcgatgc
    21001 tttttttgat ggtgccttta ttgtcctttg atttggggtc gagtgagagc aatgcctcgc
    21061 aacagtaacg gaaagtgatg atttcgtgag aaaaattctc gacttcgctc atcgaaatct
    21121 cattgttcag cacatcgagt ttcctgacaa cctggcgatt tttaaaaaag tcgatcatgc
    21181 cttccaggaa aagcacaaac ccttttgctc cggcgtcaac cacgttggcc ttggcgagtt
    21241 gttcaagttt ccttgtcgtt tctttaagcg attcggatgc tttttgatag gcttgtgtaa
    21301 gaagttcgac aaaatcatcg aacagctctt tcacggcata aataaattca gcccattcgc
    21361 gcatcaccgt gatgatagtg ccttcaacgg gatttgctat ggcttcgtat gcataggtaa
    21421 cagcatttcg taccgagcgg gcgaaattct caatggtgaa attattctca ttgacgattt
    21481 cattgctgaa accatacagg aattgagcga atatgatacc ggaattgcct cgggctccaa
    21541 ccagggctgc ttctgcaagc gccgatgcgg tgaccttgat attatcggta gggatgggac
    21601 tatcgacgat cgagcgcatc gtacttgcca gattggtgcc tgtatcggca tcggctaccg
    21661 ggaatacgtt gatcttgtta atgagtccct gattttcgaa tatgcgttgc gctcctgcga
    21721 ggaaagcgaa gtagaaactt cggccatcca aaactgtaac cagcggttta gggttttcca
    21781 aatccttcta tttaggttat cctgaagcag aggaatgctt tggtaaatag cctcaggaaa
    21841 ttcgggggca aaattagtaa ttaatcatga atgttcgggg cgatctcccg attgttcata
    21901 accagggtta cttcagtgtt ttgaatcctg agaaggattt ttcatcaata tgccggttaa
    21961 gttcgctcac aacatcctta taatcagtgc gcaggccgca atcgaatatt ttggttaccg
    22021 ggtccttgtc aatgaccatc cggagtaatt cttccgcagc cggcgtcttc tccagttttg
    22081 aatattgttc ataagtaagg ctgatgaaag cagaattggg ggaaatcccg cggttatcga
    22141 ggagccagcc gtcatgaagc tgggttggca gggcaagctt tcctccgaaa tatacatctt
    22201 tgacatccgg ataggattca acactttttt tatcagcagt aagattgatc ggcacaagtt
    22261 gagagtaatc gtttcgggtt tgatagataa tggcatgcgg accaggaatc ccggtggcgg
    22321 ctgctttttg tgatattttg cccgaatggc aacaactgat tatggctgac gacatcatta
    22381 aaaatgataa agctttgata taacttgttt tcatgggtga ataagagttt gtacaaagct
    22441 atgaaaaaca gttaaaaaaa actttgcaat tacaactttt ggaatggcgg gacattaata
    22501 tacgttatgg ttttgtgtag ccggctgagt gggggcatac tgactgaaaa aagaggattg
    22561 ttttggcaca aaacttgcta accttaaata ctgttaaaaa aaataacatt aaataattat
    22621 aagtttaatt ttgtagttct aaacattgta ataaccaacc taagtaaaat aaaatgaaaa
    22681 aagtagttct ctccttagct gtttcgatgt tagtatctgt tgctgttttt gcccagcaga
    22741 atgcaacaat atcttcagcc tccaaacctg cagccaatcc caatgctccg gtcatcactt
    22801 ttgaaaagac tacccacgac tacggcacag ttacaaaagg cggggacgga acgtgcgaat
    22861 tcaagttcac caacaccggc gttgagcctc tgatcctttc gaattgcaca gcttcatgcg
    22921 gttgcacagt gcctgactgg ccccgtgaac cgatcctgaa aggaaagtct tcggttgtga
    22981 aagtgaaata tgatacgaac cgtattggcg gaattaacaa gactattaac gttgtgtcga
    23041 atgcaaacaa ttcaccggta caattaagaa ttatcggtaa tgttgttgat aaatccgctg
    23101 caacagccta tcccgaaaaa aacctgaacc agggggctgc tcctgttgct aaataatcgg
    23161 tttaggcgtg atataaaaag cccggttcca aaaggatcgg gttttttcat gatttttcgt
    23221 tccatttcga aaagtgaatt tttcagatgg aacagaatat ctacctttgt ctgactaaaa
    23281 cgacaattaa aagctttaaa tactatgcct acaatttcac aacgaggcca gcagatgccg
    23341 gcttcaccaa tccgcaaact ggttccttat gctgaagcgg caaagaaaag gggaataaaa
    23401 gtatatcatc tgaacatcgg tcagcccgat atacgcaccc ccgaagttgc cattgaggca
    23461 atccgccggt tcgaccagaa agtaattgaa tacagccatt cggccggcaa tgaaagttac
    23521 aggcggaaac tggcgaccta ttaccaggcc aggggtattc aggttgatca taatgatatg
    23581 atcatcacta ccggtggttc cgaagctatt tcatttgcat ttaaggtgtg tttaaatccc
    23641 ggcgacgaag taatcattcc ggaaccgttt tataccaatt ataacggttt tgccgtggat
    23701 gccaatatta aagtggttcc cataacttct gatattcgtt cgggatttgc ccttccgccc
    23761 atcagcgaat ttgaaaaagc cattacgccg cgtaccaaag ccatcatggt ctgcaacccg
    23821 aacaacccta caggttacct gtattcaaaa gaagagttgc tgcaactgcg cgaactcgtg
    23881 ctcaagcacg acctttacct gtttgctgac gaggtatacc gtgaattttg ctacgatggc
    23941 aacacccact tttcggtgat gaacctcgaa ggtctggaag aacatgccgt gctgatggat
    24001 tcggtttcga aacgatacag cgaatgcggt gtaaggattg gagtccttat ctcgaagaac
    24061 aagcaattga tggcagcagc cttaaagttt gcacaggcgc gcctcagccc tccctcattg
    24121 gggcaggtta tcggcgaagc ttcaattgat acccccgcgg agtatttcaa agaagtatac
    24181 gacgagtata tagcacgccg gaattatgtg attgaagccc ttaataagat tcccggcgtt
    24241 tttgccccga tgccgaaagg agccttctat tcggtaatca gtttgccgat tgatgattcg
    24301 gataaattct gcaagtggct tctcgaagaa ttcagctatg aaaaccagac ggttatgctg
    24361 gcgccggcat caggatttta tgctactccc gggctgggca ccaaagaagc ccgtatcgcc
    24421 tatgtgctga aactggaaga cctgaagggc gccgtgaaat gccttgaaga agcattgaag
    24481 gtttatcccg gaagaattaa ataggactca ggaagagttt tgaaaaggac cggcagaaat
    24541 gccggttttt ttgattttat attactcctg agagaatttt gatccaaaat agagatggta
    24601 tgtaagatgt cattgcctga ctgaccattc cttttaatgg atgttcgtac gtaattaaag
    24661 agtattcttt ctgttgcttt cacagaagca agcctcaaag gtgattgttt tatttcgcag
    24721 cgctgatacg ttgtactgaa atccggaata aagcgggaat tactatcagg caataatcgc
    24781 taaattttta actttgaacc gggcggctga caatatgcct gtgcctttaa ttatctcgtc
    24841 aatgagtatg aaattgattt atatataccc cggattgtcg tcctttgttc ggaaggataa
    24901 atccttcttt gagactcaat ttcaagtttc tggtttgaat tttcaggtga aaaagaagtg
    24961 tcatactccc tggatgttaa tgaaggagtc cttttttctg ctgactgcta tcccctcggc
    25021 ttctattgtt gtgatacaat ttggcggtta tcattcactc ataccggcat ttttagcaag
    25081 gctgtctcat aaaccttctg tgatcgtaat gggtggtacc gattgtgtct cctttccttc
    25141 gataggatat gggaatttaa gaaaaccatt actccgctgg tttacgctga aaagtttcag
    25201 attagcatcg atgcttatac cggcaagcca gtcactggtc gaatatgatt acgattattg
    25261 tgataaggat tatcctaaac aaggatataa atatttcgac ccggggaata agacgcccta
    25321 taaagtaatc tttaacggta tttctttaaa cttgttcagg ccggttgaag gcattatccg
    25381 ggaaaagaat acttttctta cggtatgttc aaaccttgat cgaagaaatt atttcattaa
    25441 aggtatagac ctgttcattg aagccgctaa aagatttccc ggctataagt tcgtgttggt
    25501 agggaaaaca gcgtcaggat ttgaaattga aaaaccggaa aatttacggc atattccatt
    25561 cgtttcccac gacgacttac ccggattgat gtcatcatgc tctttttatt gccagttgtc
    25621 cttgtcggaa ggttttggcg tggctttgac tgaagccatg gcgtgcggtt gcattcctgt
    25681 tgtcagtaaa gtaggaattt tggatcagat agcaggtgac agcggatttg tacttgagaa
    25741 atatgataaa gacctattgg ttaaaacgat tgagaatgca gtaaattctg acgtcgtcct
    25801 gttatcggaa agggcgggga agagggctgc agcattattt catgatggca gaagattctc
    25861 ggaacttata aaagtaatca gtgatctgat agaacagaac aaacggctat aaagataggg
    25921 tgagaagtgc tgtctttacc ggtggaatgt ttgatctcca ttatcgggag ttcagcttta
    25981 gttatctttg cacctcctat ccggaattat gaataatcaa aaaaagatta ccatcattgg
    26041 cgcaggaagc ataggaacag cccttggcaa tgtgctttcg ctgcgccctg atttgaatgt
    26101 caatctttta tcggttgaat cggatgtcgt atccgatatt tcaaccctgc atatcaatca
    26161 gaaatatttc ccgaacataa ggctgaaccc atcgctcaaa gctaccaccg ctcccggggt
    26221 gctccgcgat tcggagatca tctttatcgg cattccttcc acggctgtga ttccttacct
    26281 tcaggaaatc agggatgagc tgccggaaaa tgccattctg gttaacctgg ccaaaggatt
    26341 tggaatccat aaaaaggtga ttgcgctttg cctgagagat catttcccaa atccaacctg
    26401 tgccttgaag ggcccttcat ttgcaagaga aatcattaac aatgttccca cagccctgac
    26461 gctggcatcc acaagccggg atactattca ggcagttgcg cccctttttg atggaaccac
    26521 ggtttatatc gatacgacaa ccgacgtcgc cggggttgaa gtgctgagta tcctgaaaaa
    26581 tatttatgcc atcattatag gcatcgtcga tgcccatttt cattccccca acctgagatt
    26641 cttcatcttt acaaaggctt tcaatgagat gcgaaacatc ctgctccatg tgggcggcag
    26701 ggaagaaacc atgttcaatt attgcggtat cggcgatttc gggctgacgg cgctcaacga
    26761 tatgagccgc aatcataccc tggggctgct gatagggaag ggctttttca cggaagatat
    26821 ttccggcaag gtcgttctcg aaggacgcat tgcaacggaa atattccttg aagaactcga
    26881 aaaatcgggc ttgccgcctg aaaaataccc gctgatgcgc gaattgcaac aaaccttcca
    26941 gggaaaccac aacgtttccg actttgttaa taagattatt tcctagaaaa tccggaatca
    27001 gcttccaaga tcgtttagaa cttctgccat tctccgggtt agttcccggc gggaaaactg
    27061 ccctgtgttc cggctttcag catccaggtt tccggtgaga tagagcgaaa aatattcatc
    27121 cagaatcttc cgcatactta tttcatcgct gtagtcagcg ttttttcctg cattggtttc
    27181 tttcagcagt gcagccgcat cgccgtcgtc cgggcctagg cacaaaacag ggcggcggac
    27241 agcaaggtac tcgaataact tacccgtcag gatgactttt gcctggggcg tattatttac
    27301 cagcagcaac agtacctgtg atttctgcat ttcccggatt acctctttat gcggcaggta
    27361 tccgattttc ctgacaaatt tttccagccc gttgctttgg atagcgctca tgacagtatg
    27421 atcggcgctc ccgacgagtt ttatctgtag tttttcggca aagcggctgt catccgcgag
    27481 cttctcggcg ataaccttcc agaataaaac aggattggcc agcggataaa ttgtgccgaa
    27541 atgagaaaga ctgaatttct gatcggcctg gccatacccg gtttcttcga ggtcatcttc
    27601 atcaaagcca ttcggaataa cacaataatt gcgcttaacg atacggctga attcttcttt
    27661 catgaaattt ccgaccaccg tgaccccgtc agcctgctga agtactttct tctccagtcc
    27721 ggaatgtttc ctttttgcca gccggcttaa ttttaactgg tcgaagtaat aaatgccggt
    27781 ccagggatcg cgaaaatcag ctagccagca gataccggtt atgcgtttca gctccatacc
    27841 gatcaggtgg actgaatggg gcggaccagt cgtgataatc acatctgcag gatggtccga
    27901 caagtacctg gtcaggtatc tgacggacgg ttttatccag aatttccgcg catcggggat
    27961 gaacaggttg cccctgatcc atacagacag agattctata attcccgatt ttttcttctc
    28021 cgaaaggaaa ccggagttga tccggtcgcc ctgtttcatg ccgaccagtt ttttataagc
    28081 ggtataaggt tcccagatcg gggtcttcag tacggtgata ttgtccggga tgtcagtggc
    28141 aagcgttgcg tcgaaagcgg ggatttcagg attctcgggg gtatatatga ccggttccca
    28201 cccgaattgg cggaggtgct tggtgaattt cagccaacgc tgtacgccgg cgccgccgct
    28261 tggaggccaa taataagtga tgataagggc tttcttcacc ttagttgctt ttccccggtt
    28321 ttgagtttcc ggtgatcccg ggcccgttta tttcagtttc cggatgccgg ttcttccggc
    28381 ctttgtagat catccatccg aacatggcga tcatagcaag gatcaatagg gaagaacttg
    28441 ccagcgaaat tttttcaccc gtatagtaaa cgccgggatg gaatttatat tcaatggtgt
    28501 gcttgcctgc cggaaccatc atggccctca ggatataatt agcccggaaa taaggcgctt
    28561 ccttaccatc aatataagca ttccagccct tgtcgtaata aatatcggag aacacggcca
    28621 gttgctcctt gggtgaagat gattcgtaaa caagctggtt ggggctatat gacttaagac
    28681 ttatcgtggc ggtggaatca aaagtaaaag tgtgttcctt gacaaaactc tcaaaacgtt
    28741 tgtcaaccac ggctgtttct ttgggcctga acgtattgag ggcgtttatt tcttcatcag
    28801 cattttttac catctttaca tctcttacaa accatgcatt gcccagggct tcaaaattaa
    28861 tctggggcat cggttgatgg ttttgatcag gcatgatgaa gtatttggta tttagcatat
    28921 tgaatacgtt catgttcccc tttgaaaggt gatgatcgat aatctcctgg taacggcgaa
    28981 gtttggcgcc atggtatccg ccaatggatt tgtggaaata ggatgtgctg gcatcgttga
    29041 acgggctcac cgtctggttg aaaaccctga aatacggatc tacatccttc atgatcagtt
    29101 cgtcagcctg cgtgggctga aaaggattgg cagcaatact tttgcgaaca aagaggtcat
    29161 tgttcacata gcggcggtct accgaccaga ggtcaatgac aaccagtgca atcagagcgg
    29221 ggaatacata ctttttcttc agttttccat tgatgcaggc ccatatagcc gaagcggcca
    29281 gcacgatgaa taacagcgac cggagagcat ctgagcgcaa catggacgcc cggtcatgct
    29341 gaacggcagc cattagccag tcggggaaca cggatttgag ctgttcatca gtttttgcgg
    29401 agaaattaaa caatgatccg ccaaacaagg caaagatcaa ggtcaatcct cccgtgatac
    29461 tcagcgcata gagaagtttt ttattaagct gttttttatc gatggttccg tcaaaaatct
    29521 tcgccagggc aagaaccccg aggaggggga tcacaaattc ggcaataacc agcgtcattg
    29581 atacagcacg gaatttgtta tatcccggta cataatcgag gaagaaatcg gtaagcggca
    29641 tgaaatttcg tccccatgcc agaagaaccg acaggagcgc tgctcccagc agccaccagc
    29701 ggtacttgtt gtcgacgatg aatagtccca gcacgaacag gaaaatcacg atagctccga
    29761 tataaacggg ccctgccacg ccgggctgag gtccccagta taaaggcatg tttttaatga
    29821 cctggtcggc ctggttttca ggaaccccat tgctgatcat ggctttgtag gattccgatt
    29881 tttcactcag tttgccctga gaagcgcctc ccatgaaatt cggaacaagc agggtgaatg
    29941 tttcagcctt gccatagctc cagtcagtgg cgtagtcttt gtcgaggccg gttgttctgt
    30001 tttcggcctc ggttgttaat tcggtctttc cgcgcgtggt atatttaccg tattcataag
    30061 ttgaccaaag atttgtgata ttgcacccga cggcaattac agaggcgaat atcaatacgc
    30121 cgatagattt caggaagccg ggcagccttt tttcgcggat gctgaaaatg agttcggaaa
    30181 tcatgaatat ccctacgacc atcaacaggt aataggtaat ctgcagatgg tttgcgtaaa
    30241 gttccagcga aatgaacagg gctgagagga tgcctccgag aaggtatttg ccgcgcgtgg
    30301 tgagaatgat ccccgccagt accggcgcca tgtaaccgat ggcgtgcgcc ttgctgttat
    30361 gccccgcctc caggatgatg aaaaaatacg aagaaaaccc gaatgcgaca gctccggcaa
    30421 cagcaagcca tacatcaacg cccaggacaa gcagcagaat gaaaaagccg atcatataca
    30481 ggaacaccag atcggcaggg tggggcaggc ccagtttcat gatcttatct acatatttca
    30541 ccagattatc cttgtaaaca actgatatct ggtaggccgg catgccgcca aacatggagt
    30601 tggtccacaa ggcttcctcg ccggttttgg cccggtaatc cgaaatctct ttcgacattc
    30661 cctggtaatt catggtgtcc gattgcctca ttcgttttcc ttcgagcaac gggctgaaat
    30721 acaccagcgt caaagcaaca aatatcaata ttgccagaaa gtaagggtac gactttttca
    30781 ggaagttaga ttgtgccatg ataaaattgg atttcggttc gttaaaggtt atttgatctc
    30841 ttcgtattcg gtatcttcag cttcgttata gagttgccgg gctttccggt catctatcct
    30901 tcgtatagaa atttcgcctt cttttttcag gttgtcctcc ttccgaagat cagggttttc
    30961 ctcataaaat ttttcctgaa aattgcgaag gctctttttt attaccgtcg gaatgatgaa
    31021 acgaacaagc aaagaatagc aagcatagat gattatcagg attataatca cccgcatggt
    31081 gccggtcagt gatgcctcgt tgatgaattc ctggctcata gctgaaagaa tgggctaaat
    31141 tatcattttt cccggttccg gaatgcatta cacggtgaaa actgaaagtg tgaaaaagaa
    31201 aaaaggaggc aggtataacc tcctcctgta ttgtaaggaa aatttcttat agcgctgttg
    31261 accaatgata aacagcttta attttcaggg aactactttc cttcgtcgga aacagtaagt
    31321 tttttacgtc cgcgtgccct gcgtgcagct aaaaccttgc gtccgccaac agtttccata
    31381 cggctgcgga atccgtgttt gttctttctc ttacgtatcg atggttggaa tgtccttttc
    31441 atgggtcagg tagttttagg ggcgcaaagg tatgaaaagt ttttagatta ccaaatatca
    31501 tccggaaaaa agaaaatgtc cggccagaaa ctgccggaca tttctaaatc aaggagatac
    31561 gttattttac aggaatgttt tccaaggttg tggtcaggaa tttccagaat ttttccacag
    31621 acgcgatgtt aactttttcg tccggcgagt gcggatagca aagcgtcggt ccgaaagaaa
    31681 tcatatccca gtggggatat gcgccgctca gaatgccgca ttcaagtccg gcatgaatgg
    31741 cttttatccc ggggattttt ccatagagtt tctgataaac attctgcatc gtgttcagta
    31801 taggcgaatt catattgggc ttccagccgg gatattttcc gttgagttca acgctggcgc
    31861 cggcaagttc aaatacgctc tgtatcatgt ttccaaggtc atcctgagac gaatcgacag
    31921 aactcctcag caggcattga acgcggattt caccgtcaat catttttacg ctggcaaggt
    31981 tggtagatgt ttcaaccaat ccgggcatat cgtcgctcat ccgtatcact ccgttcggac
    32041 aggcgtacac ggctttcatc agtttgtgga aggtgtcggc gtcgatcagg ctggccggca
    32101 tcgctgtttt ttcgcaggtg aatttcaggt tgggttcaac ggcagcaagt tcgctgcgga
    32161 ataaatcgtg gtgttttgct gcgcatttga ggaatgattc ctcacggtcg gcgggaatgg
    32221 taaccactgc aaacgattcg cggggaatgg cattgcggag gctgccgccg tcgatggtgg
    32281 ccaggcgcag gttatgcttt tcggcagcat ggtacaggaa gcggttcatc agtttgttgc
    32341 tgttgccgcg ttgcaggatg atttccagcc ccgagtggcc gccttttagt ccggtaagcg
    32401 aaagtttata ggcaacgaca ccctggggaa caggttcctc cctgtatttc aaggcagcgt
    32461 tggcattggt gccgccggca cagccaacat agagttcgcc ttcttcttcg ctatcgaggt
    32521 tcagcaggat ttcgcctttc aggatgcctg gtttcagacc ttcggcgccg gtcatgccgg
    32581 tttcttcatc gcaggtaaac agcgcctcaa cgggaccgtg tttcagcgtc tttgaagcca
    32641 gcacagccat ggccgaagct acgcccatgc cattgtcggc gcccagcgtt gtgccgttgg
    32701 ccgttaccca ttcgccgtcg atgtaagctt cgatggggtc cttttcaaaa tcatgatcct
    32761 tgtcgctgtt cttctgggga accatatcca ggtgaccctg aaggattacg cctttacggt
    32821 tttccatccc cggggtggca ggtttccgga tgatcacgtt tccaacttcg tcgtgaatgg
    32881 tttctagtcc gaggttcttt ccgaattcaa ccatgaattg ctggatgcga tcctcgcttt
    32941 tcgacgggcg cggaatctgg gtaaggctta caaaattttt ccaaacctct tcagggctca
    33001 gtttgttaaa ttctttgttc attatagggt tgattttaag attaatgttt atgtgggggc
    33061 aaggagaaag gagcaagggg cagggggcaa ggagcaagga gcaaggagca gggggcaagg
    33121 agaaaggaga aaaaagaact caaaactcaa aactcagaac tcaaaacgcc aacagctaac
    33181 agtttacctg cctgggcagg ctgagcgtaa gacggcaggc caaaggctaa aagccaacag
    33241 ccgggatatg aacggtagtc ggcttcactt tagctacgtc aaaagtataa aataccggcg
    33301 gacgaatgaa tatcggcagg cttatttata gagatggaca atttttttgg ttacgaagaa
    33361 ttcttcgtcg aaaaatgtat tcaggtcaaa aacgccatgt cgccatttca gatcttttag
    33421 ttccgtttgc aagtcgccgc ctttgaggta taaaactccg tttttcagcg agttgttgtt
    33481 gtttggccct attttatttc cgacccatcc caggaattcc ggtaaagtag tcacggcgcg
    33541 gcttacgatg aaatcgtagc ggtttttaat ctcctcggcg cgggtttggt aagcgaggac
    33601 attcttcagt ccgatcgccg tttttacttc attcactacc tttattttct tgccgataga
    33661 atcgaccagg gtgaattcgc tttcggggaa caggatggca agggggatgc ccgggaagcc
    33721 tcctccggtg ccgacatcaa ggatcctggt gcctgcggca aaatggatga tttttgcgat
    33781 agccagagag tgcagcacat gcctttccat gaaattgtcc atatccttgc gcgaaataac
    33841 attgatgcgg gcattccatt cggaataaag gccttgcagc gcttccaact gctgcaggct
    33901 gttggcagga atgtcgccga aatattttag gatcagttgc atgggccgct ggaattttca
    33961 ggcataaaga taggagaata tgccgaagtt gtatgcagca aagtattccc tgatccgcta
    34021 agccgctctt tagaacgatt ataatttcaa agagcagaat attttcgtct gtttttccgc
    34081 gttttacatc ggaataaatt tctaaattcg catggcagat cttgatctcg gaacattgat
    34141 ttaatggtta gtatatcagt gaagtaaata aaatcatcta ttcataaact gtaaatatca
    34201 tggaagacat tgaatggggc caactgccct ttggttacat gaaaactgac attaacgtca
    34261 gggcttacta caaagatgga aaatggagca agcttgaatt ttcagattct gaatatatgc
    34321 cggtgcatat tgctgcaaca gggctgcatt atggacagga agcatttgaa ggcctcaagg
    34381 catatatggg caaggatgga aaaatcaggc ttttccgttg ggaagataac gccaggcgta
    34441 tgcaggattc tgcaaaaggc cttctgatgg ccgtgcctcc caccgaattg ttcagggaag
    34501 ctataattga ggctgtaaag cgcaacttac gttttgttcc tccatacgga accggcgctt
    34561 cgctgtatat caggccgctg ctgttcggtt cagggaaaaa ggttg
//
LOCUS       DAOPYV010000030        34593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_1160_length_34593_cov_12.491285, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000030 DAOPYV010000000
VERSION     DAOPYV010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 34593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34593
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1160_length_34593_cov_12.491285"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1834
                     /locus_tag="PKI35_05670"
     CDS             <1..1834
                     /locus_tag="PKI35_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNX43907.1"
                     /translation="YLYALHKRIIIGYTMDNVTRLFDLLPYYTAHCKPKDDTLAVKEN
                     GTWKKFTIQDYIRSANNISYGLMALGIGKGDNIASIAFSRPEWNFIDMGILQTGAVHV
                     PLYPTISEADYKYILNHAGIRYLFIPGKEAYRKVVPIMADVPSLKGVYSVREIEGVAT
                     LDELMKFGESNPAPEKLHEIMKTIDSHDLATIIYTSGTTGTPKGVMLSHDNLLSNARA
                     VAPIMPFGEEGRAMSCLPLCHVYERMLNYVYHLTCVPVYYVENMATIGDAIREVKPWM
                     ITVVPRLLEKIYDKIINAGRDLKGIKKFIFYRAVHIGARYELNRRNGWLYSFELDLAR
                     KLVFSKWNEALGGNLKVVVSGGAALQPRLTRTFWAAKIPILEGYGLTETSPVIAVNNF
                     EKDGVAFGTVGPVIMKTEVRIADDQEILCKGPGVMLGYYKDPELTAKAIDAEGWFHTG
                     DLGRIEPKGHLRLTGRKKEIFKTSFGKYISPELIENKFKESLFIDNLIVVGENQKFAG
                     ALIVPDFTFLKSYCSIKEIPCASENEILSNPKIVKRFHKEVQRINKSLGATEQIKSYK
                     LLDREWSVETGEVTATLKMRRRFVLEKYSMQIEKLFSKDKNQGD"
     gene            1991..3784
                     /locus_tag="PKI35_05675"
     CDS             1991..3784
                     /locus_tag="PKI35_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HNX43908.1"
                     /translation="MPSVTRIFDLPGYMAKQYPGNRIIFSCSYDGVKYKYKTSDYIRR
                     SNQVAAWLIENGVKPGDNIATILLNKPEWNFIDMGIMLAGAVHVPIYPTVSESHFRYI
                     FNDAGIRLIFLENAVIWERISRICKKLDLLHAPVSIERVDGLMHFTDIMNFPVHSENL
                     EEINRRKKNIPETDVATIIYTSGTTGRPKGVMLTHRNIVSNFLAVTEIIRMNPVRSAA
                     SFLPLCHIYERMLNYMYQHEGITIHYLHNRDKLRDDISEISPEIFCTVPRFIEKLYSG
                     IMHRGLEKKGVSRMLFFWALDLAKRYEPGDSGDIWYRRQLHLARFLVFRRWKAALGGK
                     VKIIVSGGARLNPRLARIFWGAGIKVMEGYGLTETSPVIAVGSFEEGGVKIGTVGRVL
                     PGVVVKLAEDGEILCRGPNVMAGYFNRPDRTREVIDKDGWFSTGDIGRFIDGQYLQIT
                     DRKKEIFKTSGGKYVAPQVLENRFKDSPFIEQIMVVGENRKHPAAIIVPAFDHLRSWC
                     SVKGIEYTTNEQMIAHPRIVNRILEDVTAINHDLDKTEQIKKFELIADEWSVQTGELS
                     HTLKLKRDYIQKKYALLIDSMYNQPPANSAS"
     gene            3911..4657
                     /gene="gpmA"
                     /locus_tag="PKI35_05680"
     CDS             3911..4657
                     /gene="gpmA"
                     /locus_tag="PKI35_05680"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNX43909.1"
                     /translation="MKKLVLVRHGESLWNKENRFTGWTDVDLSERGIREAGDAGKVLK
                     ENGFVFSLAYTSYLKRAVKTLNIILEEMDLMWIPVKKSWRLNEKHYGSLQGLNKAETA
                     EKYGDEQVLIWRRSYDVPPAPLPLDDPRNPRLDPRYKAIGDEAPLTESLKETVERMVP
                     YWTGDIRESLKTNDQVLVAAHGNSLRAVIKYVKGISDEDIVNLNLPTGIPLVFEFDDN
                     LNFVRDYFLGDPEVIKKLMEEVANQGKAKK"
     gene            4709..5425
                     /locus_tag="PKI35_05685"
     CDS             4709..5425
                     /locus_tag="PKI35_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNX43910.1"
                     /translation="MKTWVIPDVHGCLKTLRYLVETMIMPSRGDSLILLGDMIDRGPD
                     SKGVLDYVMAFPKKGVKVTALCGNHEEAMINAFDEVMENNFWQKLGFSPRSVKEWKAF
                     GGDTTIESFGVQNIGDIPISYINWLRDLPLYLKNEKFLIVHAGFNFKKDNIFSDTHAM
                     KWIREFDIIPQKLDGRRIIHGHVPVTLDFIFMIYKSKTYQFIDLDNGVYMQGRPGYGN
                     LLALELNSMELLVQPNLDMA"
     gene            complement(5446..6783)
                     /locus_tag="PKI35_05690"
     CDS             complement(5446..6783)
                     /locus_tag="PKI35_05690"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNX43911.1"
                     /translation="MKKSLLLLLISLSLLINVRAQDKSKEVSKKGFSMGLLPAVSFDA
                     DLGFQYGGIVNLFDYGDGTLYPGYKHQVKVEVSRYTRGSGVNQLFYDSKYLIPGIRVT
                     SDISYLTEKALDFYGFNGYQAVYHPEYVDEDDPDYVSRMFYRHERKLFRFTTDFQGKL
                     PVEHLRWLGGFGIMNIKTGAVDIAALNKKLDADEQLPDTATLYDKYVDWGIIGEDEKD
                     GGNANFLKAGLVYDTRDNEANAMKGVWTEALVVASPKFLFNDRSFAKLILIHRQYFTL
                     LPDRLSFVYRIGYQGSIGGQAPFYFQPYMLSSYSTVTKTDGLGGAKNLRGIMRNRVEG
                     DGIVYGNFELRWKFIKTVIARQNIYVALNAFTDAGMVVKQVAVDESTVKNLEPYYARY
                     FDFDYTGDRLHPSVGAGLRIALNENFILAVDYGFALKKEDGLRGLYINIGNLF"
     gene            complement(6796..8148)
                     /locus_tag="PKI35_05695"
     CDS             complement(6796..8148)
                     /locus_tag="PKI35_05695"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNX43912.1"
                     /translation="MSETKIYRGTLPYYLLYVYSYLYYHIFYRRVTVTGRENIPRNTP
                     IIFAANHQNALMDALAMLYARKGKVVFLARADIFKSKFVAGLLHFLKIMPVYRMRDGF
                     ESLGQNEEIFDNAADVLRHNTPLAMLPEGNHAGFKRLRPLKKGICRIAFMAEERSQFK
                     LNLHIVPVGLDYSNYFNAGERILINFGKPIRVADYLTLYRESPQHAINALRNEIVAGL
                     KPLMLNIENEELYNTSADIIGFYQHVLLSERKLRNTHRNRFKIQQEISSRLNQITANT
                     PELLEPIRPLLSEYSGLLEKYDIRDWLVRKNNPNPLRLFAGILLSAILLPAHIYGMIF
                     NYFPYHFPVIISRKVKDRVFRSSIHFAISLFLYLVYYVILIAVFCLIFNGIWLKIAFA
                     LSLPVTGMIAFYNYRNFLKIKGKFRFIRLKSKDNETFNHLIGLRHQIMNQIKNLFNAS
                     "
     gene            complement(8304..8573)
                     /locus_tag="PKI35_05700"
     CDS             complement(8304..8573)
                     /locus_tag="PKI35_05700"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNX43913.1"
                     /translation="MGTEDELETFEIYEGTAWEVALLKSILDDNEIESIMRDASFASW
                     NLYPARAGTVKIFVAQKDFERAVKITGEFRANMQKENPEDTGDES"
     gene            8736..10430
                     /locus_tag="PKI35_05705"
     CDS             8736..10430
                     /locus_tag="PKI35_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNX43914.1"
                     /translation="MTEKRLAGYYPALIAIASALLFIPFIGRMHLFDWDEINFAECAR
                     EMLVSGDYLNVQINFRPFWEKPPLFIWMQVLSMKAFGVNEFAARFPDAVCGILSLQVL
                     YAIGKRLYDAKFGLWWVLIYASSVLSHLYFRSGIIDPWFNFFIFLSVYFFILYSEPED
                     RKTKNRNIPVMLSGVFAGLAILTKGPVGVLLVGLVIAVFYVIGILKTLRAKTDVRDRM
                     KRPKLGEILIFAIAVLLVGGSWFIVQWLSGNSAMVVKFIQYQVRLFTIPDAGHGGHWS
                     YHIWILLLGMFPASVLALKSFRVKVFENPGQRHFYKVMLILFWVVLVLFSVVKTKIVH
                     YSSLCYYPLTFLAALTVRNLLEDKGRIQGFYRFLLIFIAALFALVTLAVPLADHYKQA
                     IIDSGMIRDAFAVANLQAVVHWTGFEGLVGVLLLAGTIVSVILFRKKLKMGLPALFLT
                     SLFFVWLTILLYPYKIEQYTQRAAVDFFKSKADGKCYILNSGYFTYAPAFYAKRMPDD
                     YRRPMWLLTGNIDRPFYLVLKEPHYKKWKHLVAHMKVVEQKNGWVILERLPEETVK"
     gene            complement(10670..11857)
                     /locus_tag="PKI35_05710"
     CDS             complement(10670..11857)
                     /locus_tag="PKI35_05710"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX43915.1"
                     /translation="MQIPRIQSLLLERYLKSFPCVAIIGPRQVGKTTMARMLLSSYDA
                     DAIYLDLESHEDSRKLMFAEQYFIERQDKLIVIDEIQRNPSLFPLLRSVIDRKRVNGR
                     FILLGSASPELMAKSSETLAGRIAYLEIHPFIYPEIRQKFLFDGLWLKGGFPAVFLEQ
                     DLTVSFEMRLQFIQTCFERELPILGLSASSVTLRNLLQMIAHSQAQVVNYSEISRSLG
                     VEVNTVKRYLDYFENAFLIRRLQPYFTNSKKRLVKSPKIFIRDTGILHALFNIGDKEA
                     LDGFTGKGSSWESFVIQQIIALLKPGVNPYFYRTQDGTELDLLLVKGMQPVAGFEIKL
                     SNAPKLYRGLSTAAKDFEGAPVFVVTHSVSEDYNLAANIRVTSFDRVFKYLKELDVIM
                     DIG"
     gene            complement(12175..15090)
                     /locus_tag="PKI35_05715"
     CDS             complement(12175..15090)
                     /locus_tag="PKI35_05715"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX43916.1"
                     /translation="MKSFFKSRLVFMLVITLLSISAKQARSQSIPIDFTFSGSCVGGL
                     TYFYPDTIVMAVNGVQSVYWTFGDGDSSLLVNPVHGYANYGTYTVTLTVYADSGYTGS
                     VTHYINISPEPSAFFSYSSPNCSSSPVLFTDLSSTTYGYINTWIWDFGDGSPVDTIVF
                     PNDPNASHSFPTAGTFIVTLSVINSEGCSDTYTASVDILSAPIANFFYSPTLCEDQVV
                     QFTDASFPNGAGDIVSWNWDFGDPASGINNTSNFTNPSHIYVSPGTYNVQQIVVNFNN
                     CSDTTIKQVVIHALPSVDFSYTPALPCAFDLVFFAPIADTSSIATWYWQFGDGTASTF
                     ANPAHSYVVPGTYTVSLTVTDTSGCASSIAKSIIVNASPLANFSFPSVACSGTPVPFT
                     DLSQTNGGGAIAQYYWNFGDPASGASNTSTIQNPQHVFANAGAYIVGLTITNINGCID
                     SLFSIITVNPSPAQPSAIAGNAAPCQGSSQTYSVTNVSGITYTWALPAGWTITAGQGT
                     SSITVTTGAQSGNITVIPSNSCGNGTPASLAVSPGLIPAQTSAITGNATPCQGSIQSY
                     SVTDVSGTTYIWTVPADWSITSGQGTSSITLTTGTQNGNITVTPSNNCGSGAATTLGV
                     STGYITAYAGPDIVVSYGFPVTIYGDAGNGSGDYSWSWQPANLFVNSTVQNPTTVNLT
                     TTTVFTLTVTDNVTGCTDDDQTTVVVTGGELTVSAFANPELIYLGDSSQLNAPTSGGT
                     GNYTWSWSSDPAGFTSGLQNPVVNPVESTMYIVTVNDGVSTATDSVLLTINYLPAPPA
                     MPAGPDSVDLAYSLQTTFSIPAVANATLYEWEVYPEYAGVFAKSDVEGTISWSPSFMG
                     YAVIKVRANNSYGYSEWSEEKIIFVDNTTGTGLHESGKLIIYPNPASASLHIEIPGNL
                     SAGNGMMVIYDAKGGILSELHFTGNEMTVDVSAFLPGIYIVRFSNDKVAAIGKFVKD"
     gene            complement(15950..17344)
                     /gene="rlmD"
                     /locus_tag="PKI35_05720"
     CDS             complement(15950..17344)
                     /gene="rlmD"
                     /locus_tag="PKI35_05720"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNX43917.1"
                     /translation="MARNRTYTHIELTGMHSGGRCFGFAGGKRIYADYGIPGEKVNIS
                     GVQRQGDFLKGTVSEVTESGEYRVEPFCRHFGICGGCSWQHMSYEGQLAWKRQLVINA
                     LRKYQIDAPEVPPVIPSPGQQYYRHRLEYAFSNRRWYYEGEGRIEDPQQRLALGFHLA
                     DNPAKVVDIDECFLQQDPGRKIYQCIKEFTLQNGYSYYDPKERSGLMRSLAIRTTTTG
                     QVMVTVVFGEDNPEMRKPLFDHLLKEVPEVTSVYYGIAYAGDDNSNDIQLFHLSGTET
                     CITEKANGLIFTISPHSFYQPNPEQAGIMFKTIAGLAELQKEDLVVDLYSGIGTIALT
                     IASKCRRVTGIEGSPDACNDAVRNAGLNGITNAEFIQGDCLATFNDSFLEKYGNPDVI
                     MLDPPRSGTLIEIKKTILKAQPRKIIYLSCNPLSLAFDLKMLTQGYHISYIQPFDQFP
                     HTHHVETLVMLDKV"
     gene            complement(17432..19561)
                     /gene="scpA"
                     /locus_tag="PKI35_05725"
     CDS             complement(17432..19561)
                     /gene="scpA"
                     /locus_tag="PKI35_05725"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HNX43918.1"
                     /translation="MRPDFKNISPFVKPAGNNPAKKAEWTTNEQIAVKPYYTPDDLAG
                     MEHLNYAAGIPPFLRGPYSTMYVMKPWTIRQYAGFSTAEESNAFYRRNLAAGQKGLSV
                     AFDLATHRGYDSDHERVVGDVGKAGVAIDSILDMKILFDQIPLDKMSVSMTMNGAVLP
                     VLAFYVVAAEEQGVPMEKLSGTIQNDILKEFMVRNTYIYPPLPSMRIIADIFEFTSKN
                     MKKFNSISISGYHMQEAGATADIELAYTLADGLEYLRTGIQAGIPVDAFAPRLSFFWG
                     AGMNHFMEIAKMRAARMLWAKIVKQFDPKDPKSLALRTHTQTSGWSLTEQDPFNNVTR
                     TCVEALAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYLQEETNICKVVDPWAG
                     SYYVESLTNEIAHKAWKLIEEVESLGGMAKAIETGIPKMRIEEAAARRQAKIDSGKET
                     IVGVNKYRLDKEDPLEILEVDNTAVREAQIKRLQKLRAERNNEDVRKALDALTHAAET
                     GEGNLLELSIDAARKRASLGEISYALEKVYGRYKAVIRSISGVYSSENQDDASYHKAC
                     EMADKFAELEGRRPRIMIAKMGQDGHDRGAKVVATGYADIGFDVDIGPLFQTPAEAAR
                     QAVENDVHVLGVSSLAAGHKTLVPQVIEELKKLGRDDIMVIVGGVIPAQDYQFLYDAG
                     AVAVFGPGTKIPDAAIKMLEILIESRK"
     gene            complement(19596..21509)
                     /locus_tag="PKI35_05730"
     CDS             complement(19596..21509)
                     /locus_tag="PKI35_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNX43919.1"
                     /translation="MTSDMEIKNNKLFSEFPPVPTPEWEAKIIEDLKGADYQKKLIWK
                     TPEGINVKPYYREEDITGLSDQYSIPGKSPFIRGNSAEGNQWEIRQEIAVTDPVKANE
                     TALHAIEKGAECIGFDASRISTVDDLEALFKGISFPKVSVYFGAARSYPQLFKLLADY
                     AGKNKLPANAFRGTVDFDPISFLLLNGNFWKSQEADMAEVAKIVKLGEVLLPAMKVIT
                     VNGQYFHNSGATLVQELAFSLASANEYLAYCTGHGFTVDEVAQRLGFSFGTGSNYFME
                     IAKLRAARLLWSRIVQQYKPANENSLKTFIHCTTSHWNKTVYDPHVNILRTTTEAMSA
                     ILGGANSILITPFDTFYKDPEDFSERIARNQQIILREEAYLNKVVDPGAGSYYIESLT
                     HSIAEAAWKLFVDVEEMGGIIEAIKENFIQDSIEKVAQQRMSDIANRRTTILGTNQYP
                     NTGEIMLDKIQVIGENEDIEDTTGPAGRYKILEIGRGAEAFDDLRLNTELYVNEGGKR
                     PSVFLFAIGNLAMRKARAMFSTNFFGCAGFEIIDNNGFATIGDGVKAALASGAEIIVL
                     CSSDEEYPEFASPVVKAIKEQNNKALVVLAGYPKELIESLQQDGFDEFIHVRSNLLET
                     LRNIQDKLGISEN"
     gene            complement(21851..23578)
                     /locus_tag="PKI35_05735"
     CDS             complement(21851..23578)
                     /locus_tag="PKI35_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNX43920.1"
                     /translation="MAQVSIHREQSEYYRSLGLKSVARFDSLNDFTPMRIPADDTCSL
                     RKMVFGYHPYWAGSQYLNYRWNLLSDLCFFSYEVDPATGNATTTHDWETATVIDTAQA
                     HGTRVHLCITSFRSHQLFFSNPQAQQQLISNTISLLKLRNADGVNLDFEAVPSSLSAY
                     YNDFLVAFADSLHASIPGCLVSMASPAVDWDYDLDMPELSEHLDFFMIMAYDYYWNGS
                     STAGPVAGMFPMTGSYNYSVARTVSWYLSRGVPREKMVLGLPYYGRDWPTVKEYAPSA
                     ASGGGSALTYRQIRNNYAGHYSDNYLHFEPNSFANYYSYRTDGWHQCFLDDVHSFNRY
                     YNLVNRFGLKGIGIWALGYDDGRAELWDMIRDKLTGCAFAVCEDSLYDAGGPAFGYEN
                     NEDYRFTVAVKPGDDIFLEFDQVSMDDSFDTLFVYDGPDDSSPLINTFVAGTVAGPVH
                     SAGNAVSFHFKSDDAGTGGGWKIHYRCPSAGIDHICSLDQASVFPNPFSAGFTLSLSS
                     RACGEFYCTIFDGSGRKAINRRSLNFTPGNNSYPFFDKALSALPPGIYLMSIVFGNGR
                     SQVVKLLKQ"
     gene            complement(23837..25996)
                     /locus_tag="PKI35_05740"
     CDS             complement(23837..25996)
                     /locus_tag="PKI35_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNX43921.1"
                     /translation="MRKTFSLITFLLISLLLKAGEGMWLPVLLQQLNEKEMRDMGMRI
                     TAEDIYSVNHSSLKDAVVLFGGGCTGELVSDQGLLLTNHHCGYGSIQRQSSLEHDYLT
                     DGFWAQSQAEELPNPGLSVTFLVRMEDVTKAVLQNVLPGMTERRRDSVTASNCKKIIE
                     EAIKGTHYKGNIKPFYAGNQYYLFITETFTDIRLVGAPPSNIGKFGGDTDNWMWPRHT
                     GDFSVFRIYAGKDNNPADYSKDNVPFKPKKYLNVSLKGVSEGDFTFIYGYPGTTQEYV
                     PAAVVDMTVNHQNPIAIGLRQQRLDIIDRAMDQDRLVRIQYSAKAAGIANYWKKMIGE
                     SRGIRRLDAIDKKAAFEQEFSQWVVSDPGRKAAYGEILPRYNSIYKALNPKYDAYTYL
                     AEGPLSIEIIRYAYSFSKLVEQSIQKPAGDSAVNALAKQLLAGAKGYFKNYNEAVDRQ
                     IFAAIMKTYCEKADPAYMPEIYLQNKKHYNGNYERWSEEVFTKSLFSSLEKVQSLLEN
                     YSRSDAKKIQKDPAFKIAAATYAYIRENLSGDIYRYNDQADSLQRIYMKAQMEFQPEK
                     HFYPDANLTLRVAYGRVKGYEPADAVEYNWFSTLDGIIQKENPDIYDYVVDPRLKELN
                     RNKDYGPYGDKDGSMHVTFIATNHTTGGNSGSPVLDADGNLVGINFDRCWEGTMSDLM
                     YDPDQCRNITLDIRYCLFVIDKVAGAKRLVEEMTLIR"
     gene            complement(26170..26547)
                     /locus_tag="PKI35_05745"
     CDS             complement(26170..26547)
                     /locus_tag="PKI35_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNX43922.1"
                     /translation="MKKIIFTDKAPKAIGPYSQAVEANGMLFISGQVPIDPATGKLAE
                     GGIREQTEQVMKNIGAILEAAGYTYADVIKSTCLLSDMDNFAAMNEVYGRYYPSESPA
                     RAAFGVVKLPLGALVEIETIAAK"
     gene            complement(26584..27717)
                     /locus_tag="PKI35_05750"
     CDS             complement(26584..27717)
                     /locus_tag="PKI35_05750"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HNX43923.1"
                     /translation="MPQNIPLSMSQSNRLISLDALRGLTIIAMIIVNTPGDWSSVYPP
                     LRHAAWAGCTPTDLVFPFFLFIMGFSLFLSLQNRLKRGDSKKKIFLHLTKRSALIFFI
                     GVLLYAFPFTDLVHFRIPGVLQRIALVNFFCGLMLLYTSRKFRIYAGVIILLGYWILL
                     AFIPSPLSASLTLAYETNWAAWLDQLVLGNHIYSSMPLMDPEGILSTFPAIVTGLLGT
                     GIAAKFTETNDKTRRAVVLFLSGFLLTAAGLAWSPVFPFIKKLWTSSYVLYTAGLASM
                     TLGAFYWLTDYLKKEKYTRLLVAFGMNPLALYVGSELLIMILWLFPVFGPEKLTLTPY
                     FFNALVDAGLSPDNASLAWALAYTSVFAVAAWILYRKKIVIKL"
     gene            27845..28696
                     /gene="tatC"
                     /locus_tag="PKI35_05755"
     CDS             27845..28696
                     /gene="tatC"
                     /locus_tag="PKI35_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNX43924.1"
                     /translation="MAEENSENGLPRGKANEMTFWEHLTELRSRLLRVIIAVLVLSVV
                     AFIYSRFIFDKIILAPKSSDFITYRWLCSLGRFLHIDSLCLQKMNIEIINLNLSGQFM
                     TDMYISIFAGLLLATPYIFWEAWKFIKPALYVKEQRYARSAVFIMSGLFLIGVLFSYY
                     FMVPWTLNFLGTYQVSSSVNNQISLSSYISTVISVILSVGVVFELPVVVYVLAKIGIM
                     SSDFLKRNRKYAFVVVLIIAAIITPPDVFSQIIVTMPLWLLYEVSILVAKRVEPKEEK
                     AADQEAD"
     gene            29232..29723
                     /locus_tag="PKI35_05760"
     CDS             29232..29723
                     /locus_tag="PKI35_05760"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNX43925.1"
                     /translation="MEKTNRLYRSRTSRVFGGVAGGLAEYFDVDPIIIRLLFLILLLA
                     GGGGLLVYIILWIAIPERPLIPFEDRFNTSGETTKGESSEGAQANPGEPYFPQSRKPY
                     QPGRGGLIGGLVLISLGCIFLADRFIPNINFHDLWPMILVVIGAVLIVTNFTDHKQDK
                     KEQ"
     gene            29829..30767
                     /locus_tag="PKI35_05765"
     CDS             29829..30767
                     /locus_tag="PKI35_05765"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNX43926.1"
                     /translation="MSYRKIFWGVVLVLIGILFILKNTGVIFFNWHTILNLWPLLLIL
                     WGIALIPVRDWIKVTISIVTIVVAFFLVQQYGDNSRWNWRFDWDDNEDVVRIDKDHED
                     DDALENITQTLTEDYNPAFPYAKLDLNIGVGNFEIKDTTSKLLVLDRSGSQGTYSMTA
                     TELDSMKVINVSLNKAEFKGEIKNDVRMKLNPAPIWDLGLNIGAAEVDFDLSRFKTRN
                     IKIEGGASDIDVKIGALLPESNVNIQAGAASITLRIPKDAGCQITNNTFMASKSFDGF
                     KKINAHTYQTPGYAANPRKIKINMEAGMARIDVVRY"
     assembly_gap    31209..31308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(31367..32134)
                     /locus_tag="PKI35_05770"
     CDS             complement(31367..32134)
                     /locus_tag="PKI35_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNX43927.1"
                     /translation="MQLFYRHFGKGQPLIILHGLFGQSDNWVTIGRRLSERFSVYIPD
                     LRNHGRSPHDAIHSYPAMVDDLAGFLETCRIEKPVLVGHSMGGKTAMAFAFRYPEKVA
                     ALAVIDISPRQYPESPEVKKVISEMQQIVPEQFHTLREIEKYLQQRVADPQVRMIALK
                     NLCYETPGKPAWRLNLEAISHNLGQIFESVETDRTYNGPALFVRGEKSGYVVDDDIPE
                     IKKYFPSALIKTIPGATHWIHADAPEEIVNVLNEFLA"
     gene            32698..34107
                     /locus_tag="PKI35_05775"
     CDS             32698..34107
                     /locus_tag="PKI35_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX43928.1"
                     /translation="MNIRKLFTIILLATLIKNQNSLIAQVLPQDSLALVAFYNSTGGP
                     YWTNHTGWLTGPVSSWYGVTVEGNRVSKLAMYNNNLNGSIPSDIGNLSDLTNLALGYE
                     PMLYGVIPQEIGQILELKGLFIGNCTINGTIPNTIGNCKNIHWLQLFGNSLTGAIPPE
                     IGNLDSLLTLDLYDNQLTGPIPPEIGNCTQLVELLLNNNQLSGSIPAELANCNQIQTL
                     DLSYNQLDGEVPYELAGVTSYGFLYLNNNHLTGIPPWNNNWFLNVLAIGGNCMTFEDL
                     EPHFVGYMFYYYYPQDSMQTDIDTLLPKGSNFNIYSGSLGEYTTYYWFHNGVYMPGTE
                     NADTLKLININEADAGVYTCYAQSSICKEPNGMPMVLFRRPVTLGLVTGVEHTPLSDK
                     IIAYPNPCNDLLTIETFEPFQNATIVLTNIYGHNIKQVPWVNGSEKQTISVQGPAKGI
                     YILKLKDKNHTFTKRIVIN"
     gene            complement(34202..>34593)
                     /locus_tag="PKI35_05780"
     CDS             complement(34202..>34593)
                     /locus_tag="PKI35_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNX43929.1"
                     /translation="VMSDQIGKSETYNSKKGFPDKLRRIKYYDQERDKTLVFLTNNTD
                     LKAEEIAFLYKKRREVELFFKWMQQHLKIKSFWGTTMNAVKIQMYCAIIAYCLFALVG
                     NQLKVDRPIYEILQVRSISLLDKTPVR"
BASE COUNT         8885 a         7978 c         8601 g         9029 t
ORIGIN      
        1 ttatctttac gctcttcaca agagaataat tatcggctat actatggata acgttaccag
       61 gctttttgat ctgcttcctt actatactgc tcattgcaaa ccaaaagacg ataccctggc
      121 cgtgaaggaa aacggcacat ggaaaaagtt tacaattcag gattatattc gtagcgccaa
      181 caatatcagt tacggactga tggcgttggg tatcggcaaa ggcgacaata tcgcctcgat
      241 cgctttcagc cgtccggaat ggaatttcat tgacatgggc atcctgcaga ccggagccgt
      301 acacgtcccg ctgtatccca caatcagtga agccgactac aaatatatcc tgaaccatgc
      361 cgggatcagg tacctcttta ttcccggaaa ggaagcatac cgaaaagtcg ttcccattat
      421 ggcggatgtt ccttccctga aaggcgtcta ttcagtcagg gaaattgagg gcgttgctac
      481 gctggacgaa ttgatgaaat tcggggaatc gaatccggct cctgaaaaac tgcacgagat
      541 catgaaaacc attgattccc atgatcttgc caccattatt tacacctccg ggactaccgg
      601 cacgcccaaa ggggtcatgc tcagccacga caatctgctt tcgaatgcca gggcggtcgc
      661 gcctatcatg ccttttggcg aggaagggcg tgcaatgagt tgcctgccgc tgtgccatgt
      721 ttatgagcgg atgctcaatt atgtttatca tttgacctgc gttccggtat attatgtaga
      781 aaacatggcg accatcggcg atgccatcag ggaagtaaaa ccctggatga tcacggtagt
      841 gccgcggttg cttgagaaga tttatgataa gatcataaat gcaggccgtg acctgaaagg
      901 aatcaaaaaa tttatctttt accgggctgt ccatattggc gccaggtacg aattgaaccg
      961 gcgtaatggc tggctgtatt ccttcgagct tgacctggcc cgcaaactgg tattcagtaa
     1021 gtggaacgaa gccctgggcg gcaacctgaa ggttgtggtt tcgggagggg ctgcattgca
     1081 gccaagactg accaggacat tctgggcagc aaaaatcccg atccttgaag ggtacggcct
     1141 cacggaaact tccccggtga ttgccgtcaa caactttgag aaagacggtg ttgcctttgg
     1201 cacggtaggt ccggtgatta tgaaaaccga ggtaagaatt gccgatgacc aggaaatatt
     1261 atgcaaaggg ccgggagtta tgctgggcta ttacaaggat cctgaactta ccgctaaagc
     1321 cattgatgcg gaaggatggt ttcataccgg cgacctcggg cgtatagaac ccaaaggcca
     1381 cttaagattg actggccgga agaaggaaat tttcaaaact tcgttcggga aatatatcag
     1441 ccctgaactc atcgaaaaca aattcaagga atcgcttttt attgataacc tgatcgttgt
     1501 aggtgaaaat cagaaatttg ccggggcgct tatagtgccc gattttacat tcctcaaatc
     1561 ctattgttct atcaaggaga taccctgcgc cagcgagaat gaaatattat ccaatcccaa
     1621 aattgtcaaa aggtttcata aggaagtgca gcggatcaat aaatcgctgg gcgctaccga
     1681 gcaaataaaa tcatataaat tgcttgacag ggaatggagc gttgaaaccg gggaggttac
     1741 cgccacactg aaaatgcgcc gcaggtttgt ccttgaaaag tatagtatgc aaatcgaaaa
     1801 attgttcagt aaggataaaa accagggaga ctaaaaaaga gcaataatcg ttattatttt
     1861 tgtaggaaag tccggggacg gaaaagtcaa ggaccggata tgacattttt atcaaatccg
     1921 gattaaggat agggcttgta tcccggtttt atcaaacctt tttttatacc tgcttttact
     1981 ttttacggct atgcccagtg ttacccgaat attcgacctg ccaggctata tggcaaaaca
     2041 gtatcccggt aaccggataa ttttttcctg ttcttacgac ggcgtaaaat ataagtacaa
     2101 gacttcggat tatatccgtc gctcgaacca ggtagcggcc tggcttattg aaaatggcgt
     2161 caagcccggc gacaacatcg cgacaatact tctgaataag cccgagtgga attttattga
     2221 catggggatc atgctggccg gcgccgttca tgtcccgatt tatcctaccg ttagcgaaag
     2281 ccatttccgt tatatcttta acgatgccgg gatcagactg atatttctgg aaaatgccgt
     2341 tatatgggaa aggatcagcc gcatatgtaa aaaattggat ctgttgcatg cccctgtttc
     2401 aattgaacgt gttgacgggc ttatgcattt taccgatatc atgaattttc ctgtgcattc
     2461 agaaaacctg gaggagatta accgcagaaa aaaaaacatc cctgaaacgg atgtagcgac
     2521 aattatttac acctcaggaa ctaccgggag gcccaaaggg gtgatgctga ctcatcgcaa
     2581 cattgtttca aatttcctgg ctgtgacgga gattatcagg atgaatccgg tgcggtcggc
     2641 agcaagtttt cttccgcttt gtcatatcta tgagcgtatg ctcaattata tgtatcagca
     2701 cgaaggcatt accatccatt atctccacaa cagggataaa ctgcgtgatg atatcagtga
     2761 gatttccccc gaaatttttt gtacggttcc ccgttttatc gaaaagcttt atagcggcat
     2821 catgcaccgg ggccttgaaa agaagggcgt ctcgcgaatg ttgtttttct gggcgcttga
     2881 cctggccaaa cgttacgaac cgggagattc aggcgatata tggtaccgcc ggcagttgca
     2941 tctggccagg ttcctggtgt tccggcgttg gaaagctgcc ctggggggta aagtcaagat
     3001 tatcgtctct ggcggggcca ggttaaatcc gcgccttgcg cgcatattct ggggcgccgg
     3061 gataaaagtt atggaaggtt acgggcttac tgaaaccagt cccgttattg cggtaggctc
     3121 tttcgaagaa ggaggggtaa aaatcggtac agtggggcgc gtgctgccgg gcgtcgtcgt
     3181 taaacttgcc gaagacggtg agatcctgtg ccggggccct aacgtgatgg ccgggtattt
     3241 caatcgtccg gatcgtaccc gtgaggtaat tgacaaggac ggctggtttt cgaccggtga
     3301 catcgggcgc tttattgacg gacagtatct tcagatcacc gatcggaaaa aagaaatatt
     3361 taaaacatcc ggaggtaagt atgtagcgcc ccaggtgctt gaaaaccggt tcaaggattc
     3421 gcccttcatc gaacagatca tggtggtcgg agaaaaccga aaacatccgg cggctattat
     3481 tgttcctgct ttcgatcacc tccgttcatg gtgttctgtc aaagggattg aatataccac
     3541 caacgaacag atgattgctc atccccgcat tgtaaaccgc attcttgagg atgtgacagc
     3601 catcaaccac gacctggata aaacggaaca aattaaaaaa tttgaactca ttgccgacga
     3661 atggtcggtt caaacgggag aactatccca taccctcaaa ctgaaaaggg attatatcca
     3721 gaaaaaatat gcattgctga ttgattcgat gtataatcag ccgccggcta attcagcctc
     3781 ctgatatgat attgcgggcc gttaggggct aaactgcggg ccgttaaaat aatttagctt
     3841 aaaaaggtta attttgcgaa tcagaagtga atacggtcaa taggtggcat taatttaaaa
     3901 aatctgaact atgaaaaaac tggtgcttgt ccgtcatggc gaaagtttat ggaataagga
     3961 aaaccgtttt accggttgga ccgatgttga tttgagtgaa cgggggatcc gggaagccgg
     4021 ggacgccggc aaggtgctga aagaaaatgg ctttgttttc tcattggcct atacttcgta
     4081 cctgaaaagg gctgttaaga cgctcaatat catccttgaa gagatggacc tgatgtggat
     4141 tcccgtcaaa aaaagctgga ggctgaacga gaaacattat ggtagcctgc agggcctgaa
     4201 taaagccgaa acagccgaaa aatatggcga tgaacaggta ctgatctggc gccgcagcta
     4261 tgatgtgccg cctgcgccgc tccctttgga tgacccgaga aatccgcggc ttgatccacg
     4321 ctataaagct atcggcgacg aagccccgct taccgaatcg ctgaaggaaa ccgttgaacg
     4381 tatggtcccg tactggaccg gcgacatccg cgaatcgctg aaaaccaacg accaggtact
     4441 ggttgccgca cacgggaaca gccttcgcgc tgttatcaaa tatgtgaaag gtattagtga
     4501 tgaagatatc gtaaacctga accttcctac cggtatcccc ttggtttttg aatttgatga
     4561 taacctgaat tttgtccgtg attattttct cggagatccc gaagttatta aaaagctgat
     4621 ggaagaagta gctaaccagg ggaaagcgaa gaaatagtat ttttcaggcc ggaaatccgg
     4681 ctatatcagg gaatcattcc gccgaagtat gaaaacatgg gttatacctg atgttcatgg
     4741 ttgtcttaaa accttgcgtt acctggtcga aaccatgatt atgccttcgc ggggcgattc
     4801 ccttatttta ttgggcgaca tgatcgaccg cgggccggat agcaaagggg tccttgatta
     4861 tgtaatggcc tttcccaaaa aaggcgttaa ggtaacggct ctctgtggta accacgaaga
     4921 agcgatgatt aacgctttcg atgaggtgat ggaaaacaac ttctggcaga aactgggttt
     4981 ttctccccgc tctgtcaagg aatggaaagc ttttggaggg gataccacca tcgaaagttt
     5041 cggggtgcag aatatcggcg atatccccat cagttatatc aactggctca gggacctgcc
     5101 tctgtatcta aaaaatgaaa agttcctcat cgttcatgcc ggcttcaatt tcaaaaaaga
     5161 taatattttt tcggataccc atgccatgaa gtggatcagg gaattcgata taatccctca
     5221 aaaactggac ggccgcagga tcattcacgg gcatgtgcct gtgacgctcg attttatttt
     5281 tatgatttac aaatcaaaaa cataccagtt tatcgacctc gataacggcg tttatatgca
     5341 gggaaggccc ggatacggaa acctgctggc cctggaactt aacagcatgg agttgcttgt
     5401 ccagccaaat ctggatatgg cttgaaacga cagccttccg gaatcctaga acaggttccc
     5461 gatattgatg tacaatcccc ttaacccgtc ttcctttttc agggcaaagc cgtaatcgac
     5521 ggccaggatg aaattttcat tcagcgctat acgcaagccg gctccgacac tcggatgtaa
     5581 cctgtcgcca gtgtaatcga aatcgaaata acgggcatag tatggttcca ggtttttaac
     5641 ggtgctttca tcaaccgcaa cctgctttac caccatcccc gcatccgtga aagcgttcag
     5701 cgcaacatag atgttttgtc tggcaattac cgtttttata aatttccatc gcagctcgaa
     5761 gttgccgtat acaatgccgt cgccttccac gcggttgcgc atgatgccgc gaaggttctt
     5821 tgcgccgcca agcccatcgg ttttagtgac ggtggaataa gaactcagca tataaggctg
     5881 aaagtaaaac ggcgcctgcc cgccgatgga accctggtaa ccgatccggt atacgaatga
     5941 caggcggtcg gggaggaggg taaaatactg gcggtggata aggatgagtt ttgcgaagga
     6001 acggtcgttg aaaagaaatt tcggcgatgc cacaaccagc gcttcagtcc atacgccctt
     6061 catcgcgttg gcttcgttgt cgcgggtgtc gtaaaccagg cctgctttca ggaaattggc
     6121 gttgccgcca tctttctcat cttcgcctat gatgccccaa tccacatatt tatcgtataa
     6181 cgtcgccgta tcgggcagtt gctcgtcggc gtcaagtttc ttgttgaggg ctgcaatatc
     6241 aacggctccg gttttaatat tcatgatgcc gaaaccaccc agccagcgca ggtgctcaac
     6301 agggagcttc ccctggaagt cggtggtgaa acggaataat ttcctttcgt gcctgtaaaa
     6361 catcctgctg acataatccg gatcatcctc atcaacatat tccgggtgat agacggcctg
     6421 gtaaccgttg aacccgtaaa aatccagggc tttttcggta aggtagctaa tatcggatgt
     6481 taccctgatg ccggggatca ggtattttga atcataaaat aactggttaa cgcctgagcc
     6541 gcgggtgtaa cgtgatactt caactttcac ctggtgcttg taaccgggat acagggtgcc
     6601 gtcgccgtaa tcgaacaaat tgacgattcc gccgtactgg aatcccaaat ccgcatcgaa
     6661 cgatacggcg ggcagcaggc ccattgaaaa tccttttttc gaaacctcct ttgatttgtc
     6721 ctgtgcccgg acattgatca aaagggaaag agaaattaag agcagaagca gcgatttctt
     6781 catacgggtg aggtgttagg aagcgttaaa caggttctta atctggttca ttatctgatg
     6841 ccttaatcca ataaggtgat taaaagtttc attgtccttt gatttcaatc tgataaaccg
     6901 gaattttcct ttgatcttaa gaaagttgcg ataattataa aaggcaatca ttccggtaac
     6961 gggaaggctt aaggcaaaag ctatttttaa ccatatgccg ttgaaaatca ggcaaaaaac
     7021 tgcgatcagg attacgtaat aaacaagata cagaaacaag ctaattgcaa aatggatgga
     7081 actgcggaaa acgcggtcct ttacctttcg tgaaataatg acgggaaaat ggtatgggaa
     7141 atagttgaaa atcattccgt aaatatgagc cggcagcagg attgccgaga gcaaaattcc
     7201 ggcgaacagc cgtaaagggt tcgggttgtt ttttctgacc agccaatcgc ggatatcata
     7261 tttttcgagc agtcctgaat attcgctgag caagggtctt atcggctcca gtaattcagg
     7321 agtattagcc gttatctggt tgagcctgga tgaaatttcc tgctgaattt tgaacctgtt
     7381 ccggtgcgta ttgcgcaact tccgttcgga aagcagtacg tgctggtaaa acccgatgat
     7441 gtcggcgctg gtattataga gttcttcgtt ttcgatattc agcatcaggg gcttcagccc
     7501 ggcgacgatt tcgttgcgaa gggcgttgat ggcatgctgg gggctttccc ggtacagggt
     7561 caggtaatcg gcaaccctga tcggtttgcc gaagttgata aggatgcgtt cccctgcatt
     7621 gaaatagttg gaataatcaa ggcctaccgg gacaatatga aggttgagtt tgaactgcga
     7681 ccgctcttcg gccatgaagg cgatacggca aatacctttt ttcaggggcc tgagcctttt
     7741 gaaaccggcg tggttgcctt cgggaagcat ggccaggggc gtattatgcc ggaggacatc
     7801 agccgcgtta tcaaaaattt cctcgttctg gcccagggat tcgaaaccgt cgcgcatacg
     7861 ataaacaggc attattttca ggaaatgcaa cagacctgcg acgaatttgc tcttaaaaat
     7921 atcggcccga gccaggaaaa caacctttcc tttcctggca tatagcatag ccagcgcatc
     7981 catcagggcg ttctggtgat ttgctgcgaa aattatcggt gtgtttcggg gaatgttttc
     8041 cctccccgtg acggtaaccc gacggtaaaa gatgtgataa tacagataac tgtaaacata
     8101 caggagatag taaggaagtg ttccgcggta aatcttggtc tcgctcatgg tagggttttt
     8161 gcggtattca tgcagaaatg aacacccgcc tggggaactc attattcaaa tctgcgatga
     8221 tgaatatcgt cccgcaagtt ataaaagaaa ttaaaaggtt ctttgggaaa aggaatgagt
     8281 tgttgattgt caggcattgt tgatcaggac tcgtcgccgg tatcttcagg attttctttc
     8341 tgcatgttgg cccggaattc gccggtaatt tttacggcgc gttcaaaatc cttttgcgcc
     8401 acgaaaatct tgacagttcc tgccctcgcc gggtaaaggt tccaggatgc aaagctcgca
     8461 tcgcgcatga tcgattctat ttcgttatcg tcgaggatgc ttttcagcag ggcgacctcc
     8521 caggcagtgc cttcataaat ttcaaaagtt tccagttcgt cttctgttcc cattgtgtga
     8581 tcttttcaga tgattttaag atctaaaatt agtcaaaaat gttgtaatcg tttgacgcga
     8641 aaaggcgaat gcagcagatt ttggcaggag taaccttaag agaagttgaa aggccttacg
     8701 cccaatttct tatttttgtc caaaaatgat gcaatttgac tgaaaaacga cttgccggct
     8761 attatccggc gttaattgcc atagcttcgg cgctgctgtt tattcctttt atcgggagga
     8821 tgcacctttt cgactgggat gagatcaatt tcgcggaatg cgcccgcgaa atgctggtaa
     8881 gcggcgacta cctgaatgtt cagatcaatt tcaggccttt ctgggagaaa ccaccgcttt
     8941 ttatctggat gcaggttctt tcgatgaaag cctttggcgt gaatgaattt gcggcgcgtt
     9001 ttcccgatgc cgtttgcgga atattgtcgc tgcaggttct ctatgccatc ggaaaacggt
     9061 tgtacgatgc gaaattcggg ctgtggtggg tgctcatcta tgcttcgtcg gtgttgtcgc
     9121 acctttattt ccgctcaggc atcatcgatc cgtggttcaa ctttttcatc ttcctgtcgg
     9181 tgtatttctt tattctctat tcggagccgg aggatcgtaa aacgaaaaac cgaaatatcc
     9241 cggtgatgtt gtcgggcgtt tttgccggat tggcgatact taccaaaggg cctgtgggcg
     9301 ttttgctggt gggccttgtt attgcagtct tctatgttat cggtatcctg aaaaccctgc
     9361 gggcaaaaac cgatgtaaga gaccgtatga aacggcccaa gctgggtgaa attctgatct
     9421 ttgctatcgc agttcttctg gttggaggtt catggtttat cgttcaatgg ctgtcgggaa
     9481 acagtgcaat ggtggtgaaa tttattcagt accaggtgcg tttattcacg attcccgatg
     9541 ccgggcatgg cgggcactgg agctatcata tctggattct gctgctggga atgttcccgg
     9601 cgtcggtgct ggcattgaaa tccttccggg ttaaagtttt tgaaaatccg gggcagcgtc
     9661 atttctataa ggtgatgctg atcctcttct gggttgttct ggtgctgttt tcagtggtga
     9721 aaaccaaaat tgtgcattac agctcgcttt gttattatcc gttgactttc ctggcggcgc
     9781 tgacggttcg caaccttctg gaagacaagg gccggattca gggattttac cgtttcctgc
     9841 tgatattcat cgccgcactg tttgcgctgg ttaccctggc agtacccctg gccgatcatt
     9901 acaagcaggc gatcatcgat tcgggaatga tcagggatgc ttttgccgtg gccaacctgc
     9961 aggccgttgt tcactggacc ggcttcgaag gtctggttgg cgtcctgctc ctggcaggta
    10021 ccattgtttc ggttatcctg ttccggaaga aactaaaaat ggggttgccg gcgttgttct
    10081 taacaagcct gtttttcgtg tggcttacca ttctgctata cccttataaa atagagcagt
    10141 acacccagcg tgctgccgtt gatttcttca aatcaaaggc ggacgggaag tgttacatcc
    10201 tgaattccgg ttacttcacc tatgctccgg cattttatgc aaagcggatg cctgacgatt
    10261 accgccggcc catgtggctg ctcaccggaa acatcgacag gcctttttac ctggtgctga
    10321 aagaaccgca ttataaaaaa tggaaacacc tggttgccca catgaaagtg gttgagcaga
    10381 aaaacggctg ggtgattttg gagaggttac cggaggaaac cgtgaagtaa attacgaggt
    10441 ctggtaaaga tgcaaccgcg ggattcctgc caacagccat cccgggcaaa agatatcctg
    10501 gtgtcagatg taaaagttta tcgtctaaga aacagaatat caattttata aatgattttt
    10561 catctggaaa gtttttaccg gttttttaac acaaaaacca aaagttttag tgtttaataa
    10621 tgtgtcccgg gttaagggac ccggatcaaa aaaatagtcg gctgaagtgt tacccaatat
    10681 ccatgatcac atccagttct ttcaggtatt tgaatacacg gtcgaagctg gtcacacgga
    10741 tatttgcagc caggttataa tcctcagaaa cggaatgggt tacaacaaac acaggcgccc
    10801 cttcgaagtc tttggcggca gttgaaagtc cgcggtacag ttttggcgca ttggagagtt
    10861 ttatctcaaa accggcaacc ggttgcatgc cttttaccag gagcagatcg agttcagtac
    10921 cgtcctgggt acggtagaaa taaggattca cgccgggttt tagtaaagca atgatctgtt
    10981 gtatgacaaa actttcccat gaacttcctt ttcctgtgaa gccatcgagc gcttccttgt
    11041 cgccgatgtt gaacaaggca tgaaggattc cggtatcgcg gatgaaaatc ttaggcgact
    11101 tcaccagcct tttttttgaa ttggtaaaat agggctgaag cctgcgtatc aggaatgcat
    11161 tttcaaaata atcaagatat cgttttaccg tgttcacttc tacccctaac gatcggctga
    11221 tctcggaata attaaccacc tgggcctgcg aatgagctat catttgcaga aggttcctga
    11281 gcgttaccga agaagcggag aggccaagta tgggtaattc gcgttcaaaa caggtctgaa
    11341 tgaactgcaa cctcatctca aaactcactg tgagatcctg ttcaaggaag acggcaggga
    11401 aaccgccttt aagccataat ccgtcgaaaa ggaatttctg tctgatttca ggataaataa
    11461 agggatgaat ttccagataa gctatcctgc cggccagggt ttcggagctt tttgccatta
    11521 attcggggga tgcggaaccc agcaatataa acctgccatt tacccgtttc cggtcaatca
    11581 ccgatcgcag gagggggaac agggaagggt ttcgctgaat ctcatcaata acgatcagtt
    11641 tgtcctgcct ttctataaaa tactgctcgg caaacatcag tttccggcta tcctcatgtg
    11701 attccaggtc taaatatatt gcatcggcat cataggatga caacaacatc ctggccatgg
    11761 ttgttttacc cacctgcctg ggaccaataa tggctacgca gggaaatgat ttcagatatc
    11821 tttccagcag caaagattgt atcctgggaa tttgcatgag gatatttttg tacaaagata
    11881 atttagaatt gaaaaattac agtgtaaaac agcttataaa cctgcaatat tgcaaatgaa
    11941 atgcaaaaat gcaggaataa tgctttttcg gactctccac aggaaagtgg ttgagcagaa
    12001 aaacggccgg gcggttttgg agaggttgcc gggggaaacc gtgaagtaaa ttacgcagcc
    12061 tggtaaaggt gcaaccgcgg gatttctttc ctcccgtacc ggagtatgcc ctgcgaaaga
    12121 agggaagctg tgcctggagg caggaacgcc ggagcgttcc tgtcgctatt gccgttaatc
    12181 ttttacaaac ttcccgatgg ctgcaacttt atcattgctg aatcggacaa tatatattcc
    12241 gggcaggaat gccgaaacat ccacagtcat ttcattgcct gtaaaatgca attctgatag
    12301 tatcccgcct tttgcatcgt aaataaccat catcccgttg ccagcggaaa gatttcccgg
    12361 aatctcaata tgcagacttg cggatgccgg attgggatag ataatcagct tgccggattc
    12421 atgcaatccg gtgccggtgg tattgtcaac aaaaatgatc ttttcttccg accattcgga
    12481 gtagccatag gagttatttg cccttacttt gatgacggca tatcccatga aagaaggaga
    12541 ccaggagata gtgccttcaa cgtcgctttt cgcgaaaact cctgcatact caggataaac
    12601 ttcccattca tacagggttg cgttagcgac ggcagggatc gaaaatgtag tttgtaacga
    12661 gtatgccagg tcgaccgaat ccggccctgc aggcatcgcc ggaggggcag gcaggtaatt
    12721 gatcgtcagc agaacactgt cggttgcggt ggataccccg tcgtttaccg ttacaatgta
    12781 cattgtcgat tctacaggat ttactacagg attttgcaga ccggaagtaa agcctgccgg
    12841 atcggaactc cacgaccagg tataattacc ggtacctccg ctggttggtg cgttcagttg
    12901 agaactgtcg ccgaggtaaa ttaattcggg attggcaaat gcacttacag taagctcgcc
    12961 gccggtgaca accacggttg tctggtcatc gtcggtgcaa ccggttacat tatccgttac
    13021 cgtcaaagtg aaaacggttg tcgttgttag gttcacggtt gtagggttct gaaccgttga
    13081 atttacaaac aggtttgccg gctgccacga ccaggagtag tcaccgctgc cattacctgc
    13141 atccccgtat attgtaacag gaaatccata tgaaacaacg atatcaggtc cggcataggc
    13201 agttatatag ccggtactta cacccagggt ggtggcggcg ccgctgccgc agttgttcga
    13261 aggcgttacg gtgatatttc cgttttgcgt tccggttgta agcgtgatgc tgctggtccc
    13321 ctgtcctgaa gtaatactcc agtcagccgg aacagtccag atgtaggttg taccgcttac
    13381 gtcagtaacc gagtatgact gtatgctgcc ctggcaggga gtcgcgttgc cggtgatggc
    13441 ggaagtttgt gccggaataa ggcccgggct tacagccagg cttgccggag ttccgttccc
    13501 gcaactgttc gatggtatta cggttatatt cccgctttga gcgccggtcg tgacggtgat
    13561 gctgctggtt ccctgaccgg cggtgattgt ccagcctgcc ggcaatgccc aggtataggt
    13621 tataccgctg acgtttgtaa cggaatacgt ctggctgctg ccctggcagg gagccgcgtt
    13681 gccggcgatg gccgatggtt gtgccggcga cggattaacc gtaattattg agaaaagcga
    13741 gtcaatgcag ccattgatat tggttatggt aaggccgaca atataggctc ccgcattggc
    13801 aaacacgtgc tgaggatttt gtatggtcga agtattggat gcacccgaag ccggatcgcc
    13861 gaaattccag taatattgag ctatcgcccc gccaccgttg gtctgtgata aatcggtaaa
    13921 cggcaccggc gtcccgctgc aggcaacaga aggaaaggaa aaattcgcaa gagggctggc
    13981 atttacaatt attgatttcg caatagaact ggcgcatccg gatgtatcgg ttacggtcag
    14041 tgatacagtg taagtaccgg gaacaacata actgtgagca ggattagcaa acgtagatgc
    14101 agttccgtca ccaaattgcc aataccaggt agcaatactt gaagtatcag caattggtgc
    14161 gaaaaatacc agatcaaagg cacaaggcag tgccggcgta taggaaaaat cgacggaagg
    14221 taatgcatgg atcaccacct gtttgatggt ggtatcgctg cagttgttaa aattcacgac
    14281 gatttgctga acgttatagg ttcccggtga aacgtagatg tgactaggat ttgtgaagtt
    14341 ggaagtattg tttataccgc ttgccgggtc gccgaagtcc cagttccagc taacaatatc
    14401 accagctccg ttaggaaatg atgcatcagt gaactgaact acctgatctt cacatagggt
    14461 tggcgaatag aaaaaattag cgatcggtgc agaaaggata tctaccgaag cagtatatgt
    14521 atcgctgcat ccttcactgt ttataaccga caatgttaca atgaaagttc cggcagtcgg
    14581 aaatgagtga gatgcatttg gatcattcgg gaaaacgatg gtatcaaccg ggcttccgtc
    14641 accaaagtcc caaatccagg tgttaatata cccataagtt gtagaagata aatcggtgaa
    14701 gaggacggga gaactgctgc agttgggcga actataagag aaaaatgccg aaggctctgg
    14761 agaaatgttt atataatgtg ttacggatcc ggtatatcca ctgtcggcat aaactgtcaa
    14821 tgtaacagta tatgttccgt aatttgcata gccatgcaca ggattaacaa ggagagaact
    14881 atcgccatcg ccgaatgtcc agtaaaccga ttgcacacca ttaactgcca ttacaatggt
    14941 atcgggatag aaatacgtga gaccgcccac acaagagcct gaaaaggtga aatctatcgg
    15001 gatcgattgt gaacgcgctt gttttgcaga aatgctgagc aatgtaataa ccagcataaa
    15061 aacgagacgg gatttaaaaa agcttttcat gatctaaaaa ataaatgatg gttgataatt
    15121 aaacaattta tggttcaaat atataaaagt tttttttatt tcaccaccaa ataaaaaaga
    15181 cttaatcatc agatactgct ttgtctcctt tttcctcccg gggctcgata gcttaggctc
    15241 ttagctatta gccgctggct cttggccgtt agcctttagc ctttagccgt tagcttttag
    15301 ctattgaccg tttgccttgt gtcttagctt tttgccaggg gctgttggcc tcgccccata
    15361 tcctgagact acgttagcac tcccattccg cgaggagttt taagcccgga gggctttaaa
    15421 acgacgaaac ggtctcccag ccttaccgaa atatatgttc agctccccgc gcgccgactc
    15481 tcaccattta cttttgccct ttgccctgcc ttcttgccca caacctcacc caggcaaact
    15541 tttgactttt gccctttgcc ttttgcgttc ttaattttac gttttgcgtt ttgcgttttg
    15601 cgtttcagtg tttcagagtt tcagcgttca gcgtttttcg tcctgggtta ttattgccta
    15661 aagtacttaa agtgcctgaa gttttgagtt ttcctttgac ctttgccctg ccttcttgcc
    15721 cacaacctca cccaggcaaa cttttgactt ttgccctttg ccttttgcgt tcttaatttt
    15781 acgttttgcg ttttgcgttc cccgttcccc cgttccccgt tcttccttct ctttctctgt
    15841 tttccctgtc attctccctt ctccgttctc cctgctctct tgccctgccg tcttaaccac
    15901 aacctcgccc aggcaggtaa acttttggcc cttggctcct gaccgtatat cataccttat
    15961 ccagcattac cagcgtttcc acgtgatgcg tgtgcgggaa ctggtcaaat ggctgaatat
    16021 aagaaatatg gtaaccctgt gtgagcattt tcaggtcaaa ggcgagcgac aacgggttgc
    16081 agctcaggta gatgattttg cgcggctgcg ctttcaggat tgtttttttg atctcgataa
    16141 gcgttcccga acggggagga tccaacatga ttacatccgg gttcccgtat ttttccagaa
    16201 aggaatcatt gaaagttgcc aggcaatcac cctggatgaa ttccgcattg gttatcccat
    16261 taagcccggc gttccggaca gcatcgttac aggcatccgg cgatccttca attccggtca
    16321 ctctccggca ttttgacgct atggtaagcg caatcgttcc aatgccgctg taaaggtcga
    16381 ctaccaggtc ctctttttgc agctcagcca gcccggcgat ggtcttgaac attatgccgg
    16441 cttgttccgg attaggctgg taaaatgagt gcggactaat ggtaaaaata agtccgttag
    16501 ccttttcggt gatacaggtt tccgttccgc tcaggtggaa aagctgaata tcgttgctgt
    16561 tgtcatcgcc tgcataagca atgccgtaat ataccgaagt gacttccgga acctctttta
    16621 aaagatgatc gaacagcggc ttccgcattt caggattgtc ctcgccgaaa accacagtca
    16681 ccatcacctg cccggtggtt gtggtcctga tggcaaggct tcgcatcaac cccgaccgct
    16741 ctttgggatc gtaataggaa tatccgtttt gaagggtaaa ctctttaata cattgataaa
    16801 ttttccggcc tggatcttgt tgtaaaaaac actcgtcaat gtcgacaacc ttcgcaggat
    16861 tatctgccag gtggaatccc agggccagcc tttgttgcgg gtcctcgatc cggccttctc
    16921 cttcgtaata ccagcggcgg ttcgaaaaag cgtattccag ccggtgcctg taatattgct
    16981 gcccgggtga aggtatgacc ggcggcacct ccggcgcatc gatctgatat ttacgtaagg
    17041 cattgataac cagttgccgt ttccatgcca gttggccttc atagctcata tgctgccatg
    17101 aacagccgcc gcaaatcccg aaatgccggc agaaaggttc aacgcggtat tcgccggatt
    17161 cagtaacttc cgatacggtt cctttcagaa agtctccctg tctctgaacg cctgaaatgt
    17221 ttactttttc gccggggatg ccgtaatcgg cataaatcct tttgccgccg gcaaagccaa
    17281 aacaccggcc gccggagtgc atgccggtca gttcgatatg ggtgtatgtc cggtttcggg
    17341 ccataagggc gcagcgtttt gtgaggcttc tgtcaaattg aaaaaccggg ctgttgaaac
    17401 ccggtttttc agattgattt ttctgataag attatttcct gctttcgatc aggatttcga
    17461 gcatttttat ggcagcatcg gggattttgg tacccgggcc aaaaacagcc acggctccgg
    17521 catcatacag gaactggtag tcctgggcgg ggatcactcc accgacgata accatgatat
    17581 catcgcggcc cagcttcttc agctcttcga taacctgagg gacgagggtt ttatgtcctg
    17641 cggcaaggct cgaaacgccc agcacgtgaa catcgttttc aacagcctgg cgggcagctt
    17701 cggccggagt ctggaacaag ggaccgatgt cgacatcgaa gcctatgtcg gcatagccgg
    17761 tggctacgac ttttgcaccg cggtcgtgac cgtcctggcc catcttggca atcatgatgc
    17821 gggggcggcg gccttcgagt tcggcaaatt tatcagccat ttcgcaggct ttatgatagc
    17881 tggcgtcatc ctggttttcg gatgaataaa ctcctgaaat ggaacggatc acggctttat
    17941 acctcccata tactttttcg agtgcataac tgatttctcc cagtgaagca cgcttgcggg
    18001 cagcgtcgat gctcagttcc agcaggttcc cttcgcctgt ttcagcagca tgggtaaggg
    18061 catcaagcgc tttccggaca tcttcgttgt tgcgttcggc gcgaagcttc tgtaaccttt
    18121 taatctgggc ttcgcggacg gcggtattgt caacttcgag tatttcgagg ggatcttcct
    18181 tgtcgaggcg gtatttgttc accccgacaa tggtttcttt ccccgagtca attttggcct
    18241 ggcggcgggc agcagcctct tcgatgcgca ttttaggtat gccggtttcg atggctttgg
    18301 ccattccgcc gagcgattca acttcctcga tgagcttcca ggctttatgg gcaatttcgt
    18361 tggtgagcga ttcaacgtag tatgagccgg cccaggggtc aaccaccttg cagatgttgg
    18421 tttcttcctg caggtaaatc tgcgtattcc gggcgatgcg ggccgagaaa tcggtgggaa
    18481 gggcaatggc ttcgtccagt gcattggtat gcagcgactg ggtatgcccc agggcggctg
    18541 ccagcgcttc gacgcaggta cgcgtaacgt tgttgaaagg gtcctgctcg gtgaggctcc
    18601 agcccgatgt ttgcgtgtgg gtgcgtaatg ccagggattt gggatctttc gggtcgaact
    18661 gtttcacgat tttggcccag agcatacgcg cggcgcgcat cttggcaatt tccatgaagt
    18721 ggttcatacc tgctccccag aaaaacgaca accgaggggc aaaagcatca acggggatgc
    18781 cggcctgtat cccggtacgg agatattcca gcccgtcggc aagcgtataa gccagttcaa
    18841 tgtcggcggt agcgcctgct tcctgcatat gatatcccga tatggaaatg gagttgaact
    18901 tcttcatgtt tttggaagtg aactcaaaaa tatcggcgat gatgcgcatt gagggcagcg
    18961 gaggataaat gtatgtgttg cgtaccatga actccttgag gatgtcgttc tggatggtgc
    19021 cgctcagctt ttccatcggg acgccctgtt cttcggcagc cacgacgtag aaagccagta
    19081 ccggtaaaac ggcgccgttc atggtcatcg aaaccgacat tttgtccagc ggaatctggt
    19141 cgaacaggat tttcatatcg agaatggagt cgatggctac gccggctttg ccaacgtcgc
    19201 ccacaaccct ttcatggtcg ctgtcgtaac cgcggtgggt ggccaggtcg aaggcgacgg
    19261 atagtccctt ctggccggca gccaggttgc ggcggtaaaa ggcattactt tcttcagccg
    19321 tcgagaaacc tgcatactgg cggatagtcc agggtttcat cacgtacatc gtgctgtacg
    19381 ggccgcggag gaaaggcgga atgccggcgg catagttcag gtgctccatt ccggcaaggt
    19441 cgtcaggcgt gtagtacggt ttaacggcga tctgttcgtt ggtagtccat tcagcttttt
    19501 tggcgggatt gttcccggcc ggttttacga atggcgatat atttttaaaa tcaggcctca
    19561 taaccttata gcttattttt cggttattaa acttattagt tttcggatat tcccagtttg
    19621 tcctggatgt tcctgagggt ttcgaggagg ttggaccgga catggatgaa ttcatcgaac
    19681 ccatcttgct gaaggctttc aatgagttct ttcgggtatc cggcaagtac cacgagcgct
    19741 ttgttgtttt gttccttaat ggctttaacg acaggtgatg caaattccgg atattcttcg
    19801 tcggaactgc acagtacaat gatttcggca cccgatgcca gggctgcttt tacgccatcg
    19861 ccgatggtgg cgaaaccgtt gttatcgatg atttcgaaac cggcgcagcc gaagaaattg
    19921 gtgctgaaca tggcacgggc ttttctcatg gccaggttgc cgatggcgaa aaggaatacc
    19981 gacgggcgtt tgccaccttc gtttacatat agctcggtat tgagcctgag gtcgtcgaaa
    20041 gcctcggcgc cgcggcctat ttccagtatt ttgtaccggc cggcaggtcc ggtcgtatct
    20101 tcaatatctt cgttttcacc gatgacctgg attttatcaa gcataatttc tcccgtattg
    20161 ggatactggt ttgtgcccag gatggtggta cggcggttgg cgatatcgga catccgctgt
    20221 tgtgccactt tttcgatgct gtcctgaatg aaattttcct ttatagcctc aatgatgccg
    20281 cccatctcct caacatcaac aaataatttc catgctgctt cggctatcga gtgtgtcagc
    20341 gattcgatgt aataggaacc ggccccggga tccaccactt tattgaggta tgcctcttca
    20401 cgcaggatga tctgttgatt ccgggcgata cgttccgaaa aatcctccgg gtctttgtaa
    20461 aaagtatcaa aaggtgtgat gaggatggaa ttggcgcctc ccagaatggc cgacatggct
    20521 tcggtggttg tacggaggat gttaacatgc gggtcgtaca cagtcttgtt ccagtgagag
    20581 gtagtgcagt gaatgaaagt tttcagcgag ttttcattcg ccggtttgta ttgctgaacg
    20641 atacgggacc acaataaacg ggctgcccgc agcttggcaa tttccatgaa gtagttcgag
    20701 cctgtcccga aagaaaatcc aaggcgttga gcaacttcat caacggtaaa tccgtgaccg
    20761 gtgcagtagg ccaggtattc gttggcagag gccaggctga atgccagttc ctgaaccagt
    20821 gtggccccgg aattatgaaa atactgtcca ttgacggtga tgaccttcat ggccggcagc
    20881 aatacctcgc caagcttgac gatttttgcc acttcagcca tatcggcctc ctgcgatttc
    20941 cagaagttgc cgttgagcaa caggaaactt ataggatcaa aatccaccgt gccgcggaaa
    21001 gcgttggcgg gtagtttgtt tttaccggca taatcggcca gtaatttaaa caattgggga
    21061 tatgatcttg cggcgccaaa ataaaccgat actttcggga aagagattcc tttgaaaagg
    21121 gcttcgagat catcgaccgt tgaaatcctt gatgcgtcaa atccaatgca ttcagcgcct
    21181 ttttcgatag catggagggc tgtttcattg gctttcacgg gatcggtgac ggcaatctcc
    21241 tgacggattt cccattggtt gccttcggct gaatttccgc ggatgaaagg gctttttccc
    21301 ggaatggaat actgatcgga aagtccggta atatcttcct cgcgataata aggctttacg
    21361 ttaatacctt caggtgtttt ccagatgagt tttttctggt aatcggcgcc cttcaggtct
    21421 tcaataattt tcgcttccca ctccggcgtt ggtaccgggg gaaattccga aaaaagtttg
    21481 ttgtttttta tctccatgtc agaagtcatt tactgaataa tccgaaatgt atcggaaatc
    21541 tgaaacatga ggcaaaagta aacataaaaa ctgagccgaa aaggttatca gttaaagtaa
    21601 taacaaaagg agatccggta ttttaagtat tgcgaatcaa tatattattc tgatatttag
    21661 tattataaaa gaatatcgtc atactgagcg ggttgtcatg ccggaaaaat acgggccggt
    21721 tgccggtcgt aactatcacc aatgacattt tcccggcatt ccggccttgc tgcatattgc
    21781 cgaagtccgt ttctgcaagg ttccgatgaa atgaattttt cccggagaaa gtcccggggc
    21841 aggaaagttc ttattgcttc aataatttca cgacctgcga tctgccattt ccgaatacaa
    21901 tactcatcag gtaaattccc ggcggcaagg cggaaagcgc cttatcaaag aaaggatatg
    21961 aattattccc gggagtgaaa ttcagcgatc gacggtttat cgctttcctc ccggaaccgt
    22021 cgaagatggt acagtaaaac tcaccgcaag cccgcgagct cagcgacagc gtgaagccgg
    22081 ccgaaaacgg attcgggaaa acggatgcct gatccaggct gcagatatga tcgatacctg
    22141 ccgacggaca gcggtaatgg attttccagc cgccgccggt gcctgcatca tcggatttaa
    22201 aatgaaaaga aaccgcattc ccggcgctgt gaaccggccc ggctacggtt ccggcaacaa
    22261 aagtatttat cagcggggat gaatcgtccg ggccgtcata aacgaataaa gtgtcgaaac
    22321 tgtcgtccat cgaaacctgg tcgaattcaa gaaagatgtc atcgccgggc ttcacggcga
    22381 cagtaaaccg gtaatcttcg ttattctcat agccgaaagc cggcccgccg gcatcataca
    22441 gcgaatcctc gcaaaccgca aaagcgcaac cggtgagctt atcgcggatc atatcccaca
    22501 gttcggcacg gccgtcgtcg tatccaagcg cccaaattcc gatgcctttc agcccgaaac
    22561 ggttcacgag gttataatag cggttgaagc tgtgaacatc atccagaaag cattggtgcc
    22621 acccgtcggt gcgataactg taataattgg caaaactgtt gggctcaaaa tggaggtagt
    22681 tgtcggagta atggccggca taattgtttc gtatctgccg gtaggtaagg gctgagcctc
    22741 cgcctgaggc agccgaaggc gcgtattcct ttaccgtggg ccagtcgcgg ccgtaataag
    22801 gcagcccgag aaccattttt tcgcgcggga cgccccgcga caggtaccac gaaacggtcc
    22861 gcgctacgct gtaattatag gatcctgtca tggggaacat gccggcaacc gggccggctg
    22921 ttgaactgcc gttccaataa tagtcgtagg ccatgatcat aaaaaagtcg aggtgctccg
    22981 aaagttccgg catatcaagg tcataatccc agtcaacagc cggcgaggcc atgctgacca
    23041 ggcaacccgg gatactggca tgaagactgt cggcaaaagc aaccaggaaa tcattgtaat
    23101 aggctgaaag cgacgacggc acggcttcaa aatcgagatt tacgccgtcg gcattgcgca
    23161 gcttcagcag gctgatggtg ttgctgataa gctgttgctg tgcctgcgga ttggagaaaa
    23221 acaactggtg gctcctgaag gaagtgatac acagatgaac acgcgtaccg tgagcctggg
    23281 ccgtatcaat aacagtcgcc gtttcccagt catgcgttgt ggttgcattt ccggttgcag
    23341 gatccacctc gtaggagaaa aaacaaaggt cgctgagcag gttccagcga taattcagat
    23401 actggctccc tgcccagtaa ggatgatacc cgaaaaccat tttccggagg ctgcaggtgt
    23461 cgtcggccgg gatcctcatc ggggtgaaat cattgaggga gtcgaaccgg gcaaccgatt
    23521 tcaggcccag ggacctgtaa tactccgatt gctcccggtg gatggacacc tgggccatta
    23581 tccggtaaga cgggaaaatg gtgaatgccg tcagtatgaa aatcgctgag aaatatatag
    23641 agaatcgggt cactgaaaca ttcaccattt ttatactttt tttagctagt gtccgggaaa
    23701 caccagaaag taattgataa tcaagcccta cctcacccca acccctctcc tgaaggagag
    23761 gggcttaatc gacacatatt caatatttta tacaatccat ggctgtttaa tccggacaat
    23821 agtgactttt ttttagctac cggatcagtg tcatctcttc caccagcctt tttgcgccgg
    23881 caaccttgtc gattacgaaa aggcagtaac ggatatcgag tgtgatgttg cggcactggt
    23941 cggggtcgta catcaggtcg ctcatggtgc cttcccagca acggtcgaag ttgataccca
    24001 ccaggttgcc gtcggcatcc agaaccgggc tgccgctgtt gccgccggtg gtatggttgg
    24061 tggcgataaa agtcacgtgc atacttccgt ccttgtcgcc gtaagggccg taatccttgt
    24121 tccggttcaa ttctttgaga cgcggatcaa caacataatc gtagatatcg ggattctctt
    24181 tctgaataat accgtcgagc gtcgaaaacc agttatactc aacggcatcg gccggttcat
    24241 agcctttaac ccggccatag gcgacccgta aggttaaatt ggcgtcgggg tagaaatgct
    24301 tttcaggctg aaattccatc tgtgccttca tgtaaattcg ctgcaggctg tcggcctgat
    24361 cgttataccg gtaaatatcg cccgagaggt tttcccttat gtacgcatag gtggcagccg
    24421 caattttaaa ggcgggatcc ttctgaattt tctttgcatc gctgcgggaa taattttcaa
    24481 gcagcgactg taccttttca agggatgaga acaggctttt cgtgaagact tcttccgacc
    24541 accgttcgta gttaccgtta taatgttttt tgttctgaag gtaaatctca ggcatatatg
    24601 ccggatcggc cttttcgcag taagttttca tgatggcggc aaaaatctgc cggtcgacgg
    24661 cttcattata atttttaaaa taccctttgg cgcctgccag caactgtttt gccagggcat
    24721 tgacggccga gtcgccggcc ggtttttgaa tactctgttc aacaagcttg ctgaaagaat
    24781 aggcgtaccg gattatttcg atgctcagcg gaccttcagc aaggtaagta taggcatcat
    24841 acttcggatt taaagccttg taaatgctgt tatacctggg caaaatttct ccgtatgccg
    24901 ctttgcggcc gggatcggaa accacccact gcgagaattc ctgttcaaaa gctgcttttt
    24961 tgtcgatggc gtcgagacgc cggatgccac ggctttcgcc gatcattttt ttccagtagt
    25021 tagcaatgcc ggcagctttg gccgaatact ggatacggac cagccggtcc tgatccatgg
    25081 cacggtcgat gatgtcgagc cgctgctggc gcaaaccgat ggcaatcggg ttttgatggt
    25141 ttacggtcat atcgaccact gccgcgggaa catattcctg tgttgtgccg ggataaccgt
    25201 aaataaatgt gaaatcgcct tccgaaacgc ctttcaggga tacgttcaga tactttttgg
    25261 gtttgaaggg aacattgtct tttgaataat cagccggatt gttgtccttg ccggcatata
    25321 tgcggaaaac cgagaaatcg ccggtatggc gcggccacat ccagttatcg gtgtcgccgc
    25381 cgaattttcc gatgtttgag ggcggcgccc cgacaagcct aatgtcggtg aaggtttctg
    25441 taatgaaaag gtaatattgg tttccggcat agaatggctt aatatttcct ttgtagtggg
    25501 ttcctttgat ggcttcttca ataatctttt tgcagtttga cgccgtcacc gaatcgcggc
    25561 gtctttccgt cataccggga aggacatttt gaagcacggc tttggtcaca tcttccatcc
    25621 ggaccaggaa ggtaacgctg aggccggggt tgggcaactc ttcggcctgc gattgcgccc
    25681 agaatccgtc ggtgaggtaa tcgtgttcca gcgaactttg tctttgaatg ctgccataac
    25741 cgcagtgatg gttggtaagc agcaggccct ggtccgatac cagctcgcca gtacaaccgc
    25801 ctccgaaaag gacgacggca tctttcaggc tcgaatggtt gacgctgtaa atatcttcgg
    25861 ccgtgatgcg cattcccatg tcacgcatct ctttttcgtt gagttgctgg agcagcacgg
    25921 gcagccacat gccttcgccg gctttcagca ggagggatat cagcagaaaa gttatcaggg
    25981 aaaaagtctt tctcatgtca ggatgtattt gttggggaca cggattggga aggatgttcg
    26041 caccgacatt ccggcttgaa gaaacagaac tttattaccc gcattcaaat tgttcaacag
    26101 tgagttttta atctctcccg ctgagatcgg aatgcgttac ggcctgtgct tttccgtgtc
    26161 ctgaagtaat tattttgcag ctatcgtttc aatctcgacc agcgcgccca ggggaagttt
    26221 tacaacacca aatgcggcac gggccgggct ttccgaagga taatacctgc cgtagacttc
    26281 gttcatggcg gcaaaattat ccatatcgct cagcaggcag gtcgatttga tgacatcggc
    26341 ataggtgtat ccggcagctt ccagaatggc gccaatgttt ttcatgacct gttcggtctg
    26401 ttccctgatg ccgccttccg ccagctttcc ggttgcagga tcgatgggaa cctgtccgga
    26461 gataaaaagc attccgtttg cttcaacggc ctggctgtaa gggccaatgg ctttaggggc
    26521 tttgtcggta aagatgatct tcttcatatc ggattatgtt ttgattttta gcgtttaaaa
    26581 gtgtcagagt ttgatgacga tctttttcct gtaaaggatc caggcagcga cggcgaatac
    26641 cgaagtatag gccagcgccc aggccaggga ggcattatcg ggcgaaagcc ctgcatcgac
    26701 gagggcgttg aaaaaatacg gggtcagggt cagtttctca gggccaaaga cggggaaaag
    26761 ccacaggatc atgatcagca attccgaccc gacatacagg gccagcggat tcattccgaa
    26821 ggcaaccagc agtcgggtat atttttcttt tttcaggtag tcggtgagcc agtaaaaggc
    26881 cccgagcgtc atggaagcca gtccggcggt ataaagcaca taagagctgg tccagagttt
    26941 cttgataaaa gggaaaaccg ggctccaggc caggcctgcc gcggtgagca ggaatcctga
    27001 caggaaaagg acaaccgccc ttctggtttt atcgttcgtt tcggtgaatt tcgctgcaat
    27061 cccggtaccc agcagacccg tgacaatcgc cgggaaagtg ctcagaatcc cttcagggtc
    27121 catcaacggc atggatgagt aaatatgatt gcccagtacg agttgatcga gccaggcagc
    27181 ccagttggtt tcgtaagcaa gggtaagcga cgccgacagc ggcgaaggga tgaaagccag
    27241 taatatccag tatccgagaa gaatgataac tccggcatat atcctgaatt tacgggaagt
    27301 gtacaacaac atcagtccgc agaagaaatt cacgagggcg atcctttgca ggacgcccgg
    27361 gatcctgaaa tggacaaggt ctgtgaaagg gaaggcataa agcaaaaccc cgatgaagaa
    27421 aatcagggcg gaccgctttg tcaggtggag aaatatcttt tttttggaat cgcctctttt
    27481 cagccggttt tgcagggaga gaaaaaggga aaaccccatg atgaacagaa aaaacgggaa
    27541 aaccaggtcg gtgggggtgc aaccggccca tgcggcatgg cggagcggtg gatagacgga
    27601 ggaccagtcg ccgggggtat tcacgatgat catggcaatg atcgtgagac ccctgagggc
    27661 atccagcgaa ataagacggt tactctgcga catcgaaagg gggatgtttt gaggcataaa
    27721 attataacaa tcccccgaaa tacacctctt tattgggttt tcaaatgatt cccaaagctg
    27781 atttccatac aacgcccgat aattgctcta attggttatt tttgccatct caatacccat
    27841 aagtgtggca gaggaaaaca gcgaaaacgg attaccccgc ggcaaagcca atgaaatgac
    27901 gttctgggaa cacctcacgg agttgcgttc caggctcctc cgggtgatta tagcggtgct
    27961 ggttttatcg gtggttgcat ttatttacag ccgtttcatt ttcgataaga tcatcctggc
    28021 tcccaaaagc agcgatttca tcacttaccg atggctttgc agtctcggca ggttcctgca
    28081 tatcgacagc ctctgccttc agaagatgaa cattgaaatt attaacctga acctttcggg
    28141 acagttcatg accgatatgt acatttcaat atttgccggc ttattactgg caacgcctta
    28201 tatattctgg gaagcctgga aatttatcaa accggccctt tatgtaaaag aacagcgtta
    28261 cgcccgttca gccgtgttca ttatgtcggg gctgtttctg atcggcgttt tattcagcta
    28321 ttatttcatg gttccctgga ctctcaattt tcttggaact taccaggtga gcagttcggt
    28381 aaacaatcaa atctccttaa gctcttacat cagcacggtg atctcggtga tattgtcggt
    28441 gggcgtggtg tttgaactgc cggtcgtggt atatgtactg gcaaaaatcg ggatcatgtc
    28501 gtcggatttc ctgaaacgca accgcaaata tgccttcgtc gtggtgttga ttatagccgc
    28561 cattatcacg cctcccgatg tcttcagcca gattatcgtc acaatgccgt tatggcttct
    28621 ttatgaagtg agtatccttg tggcgaaaag ggtggaaccg aaagaggaga aggctgcgga
    28681 ccaagaagcg gattaaaggc ccgtctttca ggggtaatca ggcaggcttg taaccaattc
    28741 cgtgactctg cgggtctgtg aaatctcagt ggttcccttc gctttaacca ccaagacact
    28801 cagaacaccc aggagcactg aggattattt accacggaga actcgaaaga acatggatca
    28861 atgtatcgat tctcagtgaa cctccatgcc ctcgatgcct ccgtggttcc cttctcttta
    28921 accttacata ggagacactt agaacactta gaggcattaa ggttttttta ccacggagcc
    28981 atggaggacg cggaaacaca gagtattaca ttatgcttag tgaacctcag tgacctccat
    29041 gccttagtgg ttctcttctt tttttctact gagaaagttc aattcctgat ccgcattcta
    29101 aaaagtttat tatcagttta ttaggttcat gatggaaatg ccggttcccc gttttttcac
    29161 aaaaacagga aggcttgtaa ccaattccgg gactgagcgt caaaagatca aaacaaaaca
    29221 atttacctcc catggaaaaa accaatcgtt tgtatcgctc gagaaccagc cgtgttttcg
    29281 gcggtgttgc cggcggactt gctgagtact tcgatgtaga tcccatcatt attcgcctgc
    29341 tcttccttat attgcttctg gccggcggcg gcggattact ggtgtacatc atattgtgga
    29401 ttgccatccc ggagcggccg ctgatccctt ttgaagaccg tttcaatact tccggtgaaa
    29461 ccaccaaggg tgaatcgtcc gaaggcgctc aggccaatcc cggagaaccc tatttcccgc
    29521 aatcccggaa accttatcag ccgggccgcg gcggactgat cggcgggctc gtcctgatta
    29581 gccttggatg tatctttctc gccgacagat ttatacccaa cattaatttc catgaccttt
    29641 ggccgatgat actggtggtg attggcgctg tgctgattgt tacaaacttt accgaccata
    29701 agcaggataa aaaagaacaa tagccgggcg ttcaatggca ggcgcatccg gactgcaagt
    29761 ttctgagcgg ttctgatgcg ccccggatac cggaaataat caccgaaaac caaaaaaaca
    29821 tcaaaaccat gagctatcgt aagattttct ggggagtagt gctggtgctg atcggcattc
    29881 tcttcatact gaaaaataca ggtgtgatct ttttcaactg gcacaccatc ctgaatctgt
    29941 ggccgttact cctcattctt tggggtattg cgctcatccc cgtcagggat tggataaaag
    30001 tcaccatttc gatcgtcacc atagtcgttg cattctttct ggtccagcaa tatggcgata
    30061 acagccgctg gaactggcgt tttgattggg acgataatga agatgttgtg cggattgaca
    30121 aggatcacga agatgatgac gctttggaaa acatcaccca aaccctcacc gaagattaca
    30181 atccggcatt tccttacgcc aaactcgacc tgaacatcgg cgtcggtaac tttgaaatta
    30241 aagataccac atccaaactg ctggttctcg accgttcggg ttcccagggc acttactcca
    30301 tgactgccac cgaactggac agcatgaaag tgatcaatgt ttcgctcaac aaggctgaat
    30361 tcaagggcga aattaaaaat gacgtgcgga tgaaactcaa tccggcgccc atctgggacc
    30421 ttggtctgaa catcggcgcc gccgaagtcg actttgacct gagccggttt aaaacccgca
    30481 atatcaagat tgaaggtggc gcttcggata tcgacgtgaa aatcggggcc ctgctgccgg
    30541 agtcaaatgt aaacatacag gccggcgctg cttctatcac tctccggatt ccgaaagatg
    30601 ccggatgcca gatcacaaac aacaccttca tggccagcaa aagcttcgat ggctttaaaa
    30661 agataaatgc ccatacctat cagactcccg gctatgctgc aaatcccagg aaaataaaaa
    30721 taaatatgga agccgggatg gccaggattg acgtggtaag gtactagagc aaggagcgag
    30781 gagacaaggg gcagggagaa aggagaacga agaacgaaga acggagctag aggcagggag
    30841 cgaggggcag ggagcagggc acaacggaaa acggagcaag gagtagaggg cggggagact
    30901 cagaacacaa aacgcaaaac gcagaatgca gaacgcagaa aggagaaggg agaagggaga
    30961 acgaaaaatt atgctggcag gcagatctca aaccctgaaa ccctgcccct tcccacagcc
    31021 cagcgcaagg caggcaggtc tgaaacccta aaaccctgca actctaaacc gaaagccaag
    31081 agccaacggc taacagttta cctgcctggg caggctgtgc gtaagatggc aggctaaaag
    31141 ctaaaggcta acggctaaca gccaatagcc agaacgccga actcataacc atcaagtact
    31201 tcgagagtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc taacagccaa
    31321 tagccagaac gccgaactca taaccatcaa gtacttcgag agtccttcac gccaaaaatt
    31381 cattgaggac atttacaatc tcttccgggg catcagcatg aatccagtgc gttgccccgg
    31441 gaattgtttt tatcagggct gaagggaaat atttttttat ctcaggtata tcatcatcaa
    31501 caacataacc ggatttttct ccccggacga aaagggcggg gccgttatat gtccggtcgg
    31561 tttctacgct ttcaaaaatc tgtccgaggt tgtggctgat ggcctccagg ttcaatcgcc
    31621 aggccggttt cccgggtgtt tcataacaga gattcttcag ggcaatcatc cgaacctgcg
    31681 gatcggcaac gcgttgctga agatattttt caatttcccg gagtgtatga aattgttcag
    31741 ggacgatctg ctgcatttcg ctgatgacct ttttaacttc cgggctttcg gggtattgcc
    31801 ggggagagat gtcgataacc gccagcgcag ccactttttc aggatacctg aatgcaaagg
    31861 ccatcgctgt tttgccgccc atcgaatggc cgacaagaac cggtttttcg atgcggcagg
    31921 tttcgaggaa cccggcaagg tcgtcgacca tagcagggta ggagtgaatg gcatcgtgcg
    31981 gcgaccgccc gtggttgcgc agatcgggga tataaaccga gaacctttcg gaaagccttc
    32041 tgccgatggt tacccagtta tcgctctgac caaataatcc gtgaaggatg ataagaggtt
    32101 gccccttgcc gaaatgccgg tagaataatt gcataggttt ttaatgccaa aaataccggc
    32161 tataaaaata gagaattccc agccctccgg caaacggcca ttgacctgta tgaatccatg
    32221 tgcgcagttg gctgtatgtg aatggaaccc ggtagaagaa gatggcttag agttgcccga
    32281 agacaacgcg aaaatcatgt gcctgccaaa aaaatgcccg gctgttcagg aagtataaca
    32341 acagacatat acaacaatat aaccaccaga aattttaatg aaataaaaag tctgacagat
    32401 aatttcgatt tttaaccata ttatataaaa acaaattcta atttagtagc cgtaatacaa
    32461 cttatttggt agagagatca gggatgcgaa caaagtataa atacccgcaa tagttcgcat
    32521 atttattcgc cggcagtaag acgaagaaaa aaacaaacgg ccacccgcag caaaagagtt
    32581 aaattttact acacaaaagc cggcattaag aatttgcaac acatttactc caggctgata
    32641 cataagataa ctttattaaa acgaacagat taactttata tataacacat cctgccaatg
    32701 aatatccgca aattattcac aattatttta ctggcaactt taataaaaaa ccaaaatagc
    32761 cttattgccc aggtgcttcc ccaagactcc cttgccctgg tagcctttta caacagtacg
    32821 ggcggcccct actggactaa ccatacgggc tggctcacag gaccagtgag tagctggtat
    32881 ggggtgacgg tggaaggaaa tcgagtgtca aaacttgcca tgtacaataa taatttaaat
    32941 ggttcaattc cgtcggacat tggtaactta agcgacctta caaatttagc acttggttat
    33001 gaaccgatgc tttatggggt gattccgcaa gaaataggac agatattaga attaaaggga
    33061 ttattcattg ggaattgtac aattaatggt acaattccca acaccattgg gaattgtaaa
    33121 aatattcact ggttacaatt gttcggaaat tcgcttactg gtgccattcc gccagaaatt
    33181 gggaaccttg acagcttact gactttagat ttgtacgaca accaattaac cggtcctatt
    33241 ccaccagaaa taggcaattg cactcaattg gttgaattgc tccttaacaa caaccagctt
    33301 tcaggctcaa ttccggctga actggccaat tgcaaccaaa ttcaaacact tgatttgtca
    33361 tataatcagt tagatggaga agttccttat gaactggcag gagttacctc gtatggattt
    33421 ttgtatttga ataataacca ccttaccgga atcccaccct ggaacaataa ttggtttttg
    33481 aacgtccttg caatcggagg caattgcatg acctttgaag accttgaacc gcattttgta
    33541 ggttatatgt tttactatta ttatccgcag gatagtatgc aaaccgatat tgatacactg
    33601 ttgcccaaag gtagcaactt taatatatac tcaggcagcc tgggcgaata tactacctac
    33661 tactggtttc ataacggggt atatatgccc ggcactgaaa acgccgacac gcttaaactc
    33721 atcaatataa acgaagccga tgcaggagta tatacttgtt atgcacaaag ctcaatatgc
    33781 aaagagccca acggcatgcc tatggtgctg tttaggcggc cggtaacgct tggcttagtt
    33841 acaggggtcg agcatacgcc attatcagat aaaataatcg cttatcccaa tccttgtaat
    33901 gatttactaa caattgagac ctttgaacca tttcaaaatg cgacaatagt tttaacaaat
    33961 atatacgggc acaatataaa acaagtaccc tgggtaaacg gatctgaaaa gcaaaccata
    34021 agtgtacaag ggccggcaaa gggtatttat attctcaagt taaaagataa aaatcacacc
    34081 ttcacaaaga ggattgtaat aaattaatca gcaaaccatt agtgggcatt aaaaacccac
    34141 tgattcttga ctggttgaaa tgtagttctt tgacattttt gtaatcacag tgcgtaagta
    34201 tctatctgac aggtgttttg tccagcagag aaatgctgcg aacttgtaaa atttcgtaga
    34261 tagggcgatc aactttgagc tgatttccaa ccaaagcaaa aaggcaatat gctatgattg
    34321 cgcaatacat ttggatctta acggcattca tggtggttcc ccagaatgac ttgatcttca
    34381 gatgctgttg catccatttg aaaaacagtt caacctcccg tcgtttctta tagaggaatg
    34441 cgatctcttc ggccttcaaa tctgtattat tggtcagaaa aactaaagtt ttgtcccttt
    34501 cctgatcgta gtatttgatt cggcgtagtt tatctgggaa tcccttcttg gaattataag
    34561 tttctgactt gccgatttga tcactcataa cac
//
LOCUS       DAOPYV010000031        32037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_1318_length_32037_cov_11.619067, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000031 DAOPYV010000000
VERSION     DAOPYV010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 32037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32037
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1318_length_32037_cov_11.619067"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..2352)
                     /gene="recG"
                     /locus_tag="PKI35_05785"
     CDS             complement(247..2352)
                     /gene="recG"
                     /locus_tag="PKI35_05785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNX43930.1"
                     /translation="MTVDILDTPIEFLKGVGPKRAEIMRSEFGISTFYDLLTYYPFRY
                     VDRSRFFSIGEINSDAAWVQLKGRVTGTQLAGKGVSMRYIVTVADETGSIDLVYFQGI
                     KWVKDKFQSGTEYVIFGKPSLYNGRYNIPHPEVETAAEFYAQPADPLHPLYSTTEKSK
                     LHGITSRAMAKLAKTLVLQVHGMIPETLTREIMDELKLIPRDEALANIHFPSDHTMLA
                     KAEARIKFEELFFIQLRLLRYKLFRSRTVKGHRFAIIGKNFNTFYSQYLPFALTGAQK
                     KVLKEIRADMGSGKQMNRLLQGDVGSGKTVVALMSMLIALDNGYQACLMAPTEILANQ
                     HFKTISKLLDGTGITCDLLTGSTRQKQRKEMFSLLRNGELHILIGTHALIEDTVQFSN
                     LGLVVIDEQHRFGVAQRAKLWSKSADPPHILVMTATPIPRTLAMTLYGDLDFSVIDEL
                     PPGRKPIKTYHFFETGREKVYNFMLQQIKSGKQIYVVFPLIQESETLDMKNLEDGYQH
                     IVEAFPPPAFRVSIVHGRMKPDEKDAEMQRFSKGDTNILVATTVIEVGVDIPNASVMV
                     IESAERFGLSQLHQLRGRVGRGADQSHCILLTGYNLGPEARTRMETMVRTSNGFEIAN
                     VDLKLRGPGDLEGTRQSGVLDLKLADIIRDEAILRYARRVASNLLNEDPSLEQIKNRP
                     LAEQLKYLSRKNADWSMIS"
     gene            complement(2454..3308)
                     /gene="gldN"
                     /locus_tag="PKI35_05790"
     CDS             complement(2454..3308)
                     /gene="gldN"
                     /locus_tag="PKI35_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HNX43931.1"
                     /translation="MKKSVLIMALLVFFIATGASAQQLLNTPPRDGVVDEGDRLEKNP
                     IPYPWVRYADFVWKKRIWRMVDVREKINQTFYYPETPHNNWKNFMTVIMDALKEGSIT
                     AYDAASPTDEFIVPLTYKELMANLERTDTVPMQRPYPPYDWYDTVMSKKFNPLDVKRF
                     RIKEDWYFDKQRSMFEVRILGICPVRDNYDENGNFRAYEPLFWIYFPEARAVFAKAEV
                     FNRFNNAARLTYDDLFWKRMFNSYIYKEENVYDRKIAEYATGIDAQLEAERIKEDLFN
                     FEQQLWEY"
     gene            complement(3333..4886)
                     /gene="gldM"
                     /locus_tag="PKI35_05795"
     CDS             complement(3333..4886)
                     /gene="gldM"
                     /locus_tag="PKI35_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HNX43932.1"
                     /translation="MAGYKETPRQKMIAMMYLVLTAMLALNVSVEILNAFLVVNESME
                     TTNRVFRAKTENLYRQFEIQNMQNPAKVGPYFDKAMKVKKLSQEMRDYVENTKFEAIA
                     FSDGISMDAARTTPLRQLASKDKYDKTTNFFIGNSQDGSKGRSRQLKDKIIKYKQDML
                     QMIEPAKRNQIKLGLDTDGPYYDASGAKQNWEMYNFYHTILAADVTIMNKIVAEVLNA
                     EQDVVTHLLSEITASDYKFDAVGAAVVPKSQYVFLGENYEAEIFVTAYDTKQTFTANI
                     GGRGVSSTEGVAKISIPATTPGPVSLKGFVNVKSPSGEMQNYPVAVDYIVAPPTLTVS
                     PTKMNVFYAAVDNPVSISAGGVSDAQLQASISNGSITRSGRGWNVRVNETGKAVISVS
                     ARMGDRAKSLGAAEFRVKRVPDPTAYIANTSGGPASQQMVLASALIPRMPADFDFDLT
                     FEVTGFTCSGVRRGDFWSRPARGNRLTEEMRDFIRGCKKGDKIWFEDIYAKGPDGTRK
                     LNSIIITLQ"
     gene            complement(4942..5991)
                     /gene="gldL"
                     /locus_tag="PKI35_05800"
     CDS             complement(4942..5991)
                     /gene="gldL"
                     /locus_tag="PKI35_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HNX43933.1"
                     /translation="MGLTSFVSSKGYKNFMSKLYGWGASLVILGALFKINHYTGANEM
                     LILGMCTEAIIFFFSAFEPPHVEPDWSLVYPELAGMYHGGAGTPSAAPHKVHTATKST
                     TQELDDMLSKAKIGPELIESLGNGMRKMSENVAKMSDVTNAAAANSEFVKTVQDASKS
                     AGELAVSYKKTADALNQDANSVGEFSSSVKSAATSAGTLATTYNDVSNSIKKELSATE
                     QFSNTMAVASESANRLTEKYNKAAELLSKSAESLNFTALDSKSYNDQLQKISHNLSAL
                     NAMYEMQLQSSNEQLEVGNKLRASFNSFLGSINETSGNMDKYKEEVEMLTKRVTALNQ
                     VYGNMLAAMNVNPNK"
     gene            complement(6088..7446)
                     /locus_tag="PKI35_05805"
     CDS             complement(6088..7446)
                     /locus_tag="PKI35_05805"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNX43934.1"
                     /translation="MKKLLLFLLVALVLGSCRNTGNGELIGVQDRERFYQPDPFGMTY
                     VPMGSYTMGPGDQDVPYAHLNTPRTITVKAFWMDETEITNNEYRQFVYWVRDSIARRI
                     LGDVNAEDFLISENKKTGETYDPPFLNWETEIEWQSEEYRDQLESMYLPEHERYFRKK
                     EIDTRKLFYEYYFIDLKAASRKDYSGQTDSKNAGFANRPQGMKDRSVYIRKETVNVYP
                     DTLAWLHDYAYSYNDPMVEQYFWHPVYDNYPVVGVNWKQASAFCIWRTRLMDSYLARK
                     NNASLSEFRLPTEAEWEWAARGGNSFSAYPWGGPYTRNDKGCFLANFKPLRGNYIDDG
                     GATPVIVAHYPANDFGLYDMAGNVSEWCSDAWDESSSNFTWDMNPTYQYNSKEDDPPA
                     LKRKVIRGGSWKDISYYIQVSTRAYEYQDSSKCYIGFRCVQDFMGRQKDDNPSRASRI
                     YN"
     gene            complement(7679..8608)
                     /locus_tag="PKI35_05810"
     CDS             complement(7679..8608)
                     /locus_tag="PKI35_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNX43935.1"
                     /translation="MLLMINCPFAEIYAQQEPQFTHNMFNYSDVNPGHYGLSEGICVT
                     GILREQWLGFKDEDGNKVNPQTLLIGADSPIKFLHGGIGLNIVQDKYGFYTDMAVKLG
                     YAYHLTVGNGVLGIGVNAQLLNKALDMNKLHGVDEGDPQLSGKSSQGVVITDMSAGIF
                     YNNPKYYLSLSSTQLLQSEKDLGGAEGVGTFTLRRHYYAGGGINLASPAFPAFILTPS
                     VFVKSDGSTLQADFNGMVTYNKKVWGGVSYRLSDAIALMVGLNAKELEIGYAYDIPTS
                     RVGATGSHEIMLRYIFRIEREKSRSGYRNTRYL"
     gene            9157..9546
                     /locus_tag="PKI35_05815"
     CDS             9157..9546
                     /locus_tag="PKI35_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43936.1"
                     /translation="MAKLNQFDPSKVSSDIVLKNSAEVYTSDNSRAFAFNEVQKNAVS
                     CLIYGGVSEDKKCPKWFSQKILQWDAATKQWKTYFDSGIKYNSVIAEDHKVAAGSWYY
                     TIQWVWDPTANDWIKFDADLLFNAVNG"
     gene            9706..10626
                     /locus_tag="PKI35_05820"
     CDS             9706..10626
                     /locus_tag="PKI35_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNX43937.1"
                     /translation="MMFSGNAKLFSKRWFIDYGLITLGSFILAAGFVFFISPYRIVPG
                     GVYGISIVIHYITKGIFSWAPEGFPIGLMGLILNIPLTIIGIKVLGPKFGVKTVVGFV
                     LSSVFMDGITMFWGERPLVEGDALLSSIFGGVLIGAGLGLIFKSKATSGGSDIIAMII
                     AKYTNIPLGQLMIYVDSTIVLLGLVVFADWKIPLYSWIVIYVTGKTIDVVIQGMSVDK
                     TLFIISDKFEEIRDSIINDLQRGGTYIPGSGMYNRASKTIIFTVVNRREMAILEEYIH
                     KVDPKAFVTVLEANEILGEGFKSLRDKISD"
     gene            10866..11777
                     /locus_tag="PKI35_05825"
     CDS             10866..11777
                     /locus_tag="PKI35_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNX43938.1"
                     /translation="MIKSPSFLQPEKIFSANWFRSYSYIVIGTFIMACGYVLFISPEK
                     IVPGGVYGIAIMLHHLLGLPIGLTALAFNIPLTIIGTRILGPRFGVKTVVAFVLTSVF
                     VDLLSYFHGNAPLVEGETLVSGIFGGLLVGIGVALTFKAKATSGGSDVVAMILAKYTH
                     RPLGQLMMIVDSSIVLLSFLAFGDWRIPLFSWITIFVIGRVVDTVMHGFSYDKTLFIV
                     SEKYNQIRDSIITDLHRGGTFLHGEGMYNQAQKQIIFTVVNPRELSMLQEYIHRIDPD
                     AFVAVLDAYEILGKGFKSLSEKVEDQS"
     gene            complement(11790..13112)
                     /gene="rseP"
                     /locus_tag="PKI35_05830"
     CDS             complement(11790..13112)
                     /gene="rseP"
                     /locus_tag="PKI35_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNX43939.1"
                     /translation="MEILIKTVQLLFSLTILVFIHELGHFLSAKACKARVEKFYLFFD
                     PWFSIFKFKKGDTEYGIGWLPLGGYTKISGMIDESMDTEQMKQPAQPWEFRAKPAWQR
                     LIIMVAGVIMNIVLAIGIYIATLAIWGEQYLPTSEVKYGIAVDSLGYELGLRNGDKII
                     SIDNAVVEDFSKIPSHILLEKARTIQVERDGKPAEVVIPEGFLSKMIKSQSADFISVR
                     IPFEVDDFAKSSPAKAAGLKKGDKIIGIDTLSTPYFDMFRAEIQKHKNQEVTVVALRE
                     NDTVRIRVKVPEKGLIGVAPKPPVTYFNLSERHYNAFQAIPAGFVKTWKGIDNYWKQL
                     KLIFSKDVKAYESVGGFITIGKIFPATWDWESFWALTAFLSIMLAILNILPIPALDGG
                     HVLFVLVEIITGRKPSDKFLEYAQIVGMVLLFGLLILANGNDIIRLFR"
     gene            complement(13166..14329)
                     /locus_tag="PKI35_05835"
     CDS             complement(13166..14329)
                     /locus_tag="PKI35_05835"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNX43940.1"
                     /translation="MKRQIAVLGSTGSIGTQALDVIRSHPRELDVEVLTAHSNAALLI
                     RQAIEFKPNAVVIGMESLYQRVFEALDPYDIKVYAGHEAICQVVEMETIDMVLVALVG
                     FQGLKPTLNALKAGKAIALANKETLVVAGEMVTGLAQSKGINIYPVDSEHSAIFQCLA
                     GEFHNPVEKIILTASGGPFRGKDRSYLATVTKDEALKHPNWSMGNKVTIDSASLMNKG
                     LEVIEAKWLFGLPPSRIEVVVHPQSIVHSLVQFTDGSIKAQLGLPDMRLPIQYALGYP
                     RRLTSDFPRFSFTKYPQLTFENPDPDTFRCLPVAYEALAKGGNMACIMNAADEVAVNA
                     FLRDEIGFLQIPDLIESCMEKISFVAAPSFGDFVATDTETRLVAQELIKSLAR"
     gene            complement(14373..15266)
                     /locus_tag="PKI35_05840"
     CDS             complement(14373..15266)
                     /locus_tag="PKI35_05840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNX43941.1"
                     /translation="MSGIKEEGEKIWQVLQERLRVKYRLVILNEETFEEKLSFKLTRM
                     NVFVVLGLLSIVLVFITSYIIAFTPLREYIPGYTDVAMQRRIYELQMRSDSIERAMES
                     NQLYLQNLRLVLGDSLSVQKKAKSDTAIKKSYTDIFDKRSPEDSLLRLDYENQSKYNL
                     FYSDAPSEKSSATSRSYTFFTPVKGVISSKFNPLLKHFGIDIVANTDEPIKAVQDGTV
                     IFSGWTVETGNVLAISHPGNIISVYKHNSVLLKKEGSVVRAGETVAIIGESGELSTGP
                     HLHLELWINGSPVNPADYILF"
     gene            15739..18483
                     /locus_tag="PKI35_05845"
     CDS             15739..18483
                     /locus_tag="PKI35_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX43942.1"
                     /translation="MKFRTLSRYILIPALFLGLALTFSCSTKKNTFTRRAFHNVNTHY
                     NVYWNGNESFKTGVTELQSAVRDNYNKVLPVFNYGTPEEAKKVTGRMDRAIEKATIGI
                     RRHSMVFGNKEYNRWIDDCFMLIGKAQFYKQDYSTAIRTFEHIMSEYQGKPVYYEALL
                     WIAQANIQQKRYEEALVRLDELDGLLTRETVPFRIRKTLPLVYADYYLRTKNVNAARN
                     ALLQGIELNTGGKTRVRLYYILGQLYQQEKNFAMSTEYFTKAAKGPASFEMAFNARIS
                     LARSFDVYSGDKAGLEKELRKMLRDGKNKDFRDQIYYALAELSQIDQNDTLTMSYLKK
                     SVATSTDNDFQKTTSALKLAGMYFDRQNYEMAQSYYDTTLQVIPLDYPDYNAINSRTV
                     TLTELVQNLQVVKYEDSLQRLAGMPDEQLNAIIQKIVEDYKKEEQRRKEEEELQQSQM
                     AAPTNALNMRNENIQNIGGGGWYFYNPSAISFGYSEFIRKWGRRKLEDNWRLSNKRPV
                     VSADETEVAENEPSDSASNIVDSVKFVKKSTDPKDPKTYLQQLPRTPEALALSNKHIE
                     DALTNLGYIYKDGLQDTLNSIQTFENLIKRFPDSKNAQRIYYQLYLIYSGIPDSANAE
                     KYKNTILNLFPGSEYARTLQDPDYLAQILARKNRALSLYEETYQAFERGQYRMVMLYC
                     NEAISSYKDKDLLPRFEYLRAISQGKTVSIDTMLVSLNNIVVDYPSSPIIPLVQEVLQ
                     KYSKKQPPAASPAATAQAATTGNQQGSAPAQAPASAPATTGSIFTSDTIVPDIYRFNP
                     KQTHFFIMMVDQNTVNINALKVRIADFNLKSFPNDNITVNAIVLESGWQMLTVSSFGN
                     SEKAMNYFNTIMQSDYVLSQLKTANYRSFVISMENYPVFYREKKYQGYINFFNKYYKG
                     "
     gene            18604..19002
                     /locus_tag="PKI35_05850"
     CDS             18604..19002
                     /locus_tag="PKI35_05850"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNX43943.1"
                     /translation="MAITKPTLPETPSVNLLGSGTEINGDIRSNGDIRIDGSLNGTIN
                     CSGKVVIGSTGRIEGELICQNADISGEVKAGVRVAELLTLKATAVLCGDIITNKLAIE
                     PGAVFTGTCKMESQNSVNGKTTDEAQTEPA"
     gene            19207..19587
                     /locus_tag="PKI35_05855"
     CDS             19207..19587
                     /locus_tag="PKI35_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43944.1"
                     /translation="MKTTLSRFFRNLIVFTLVVAAACAALFIFIPALCSPMLPWLLAF
                     FFVITLAVFYISLKASREKLSRFVNFYMVMSMLKIFMLLVVTAIYLYLRRDDALRFSV
                     SLLVLFFAYLIFEVIWLLKIRGDN"
     gene            19709..20122
                     /locus_tag="PKI35_05860"
     CDS             19709..20122
                     /locus_tag="PKI35_05860"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNX43945.1"
                     /translation="MIYITRRERFNAAHRLFRTDFSDERNAEVFGKCSNPLWHGHNYE
                     LFVTLKGEPDPETGFLVNLKALSSLIGQLIIEKLDHHNINLEVDFMKDKLASAENLAI
                     GIWNELAAPVRNMGAELYCVKLCETENNFVEYFGN"
     gene            20219..20854
                     /gene="folE"
                     /locus_tag="PKI35_05865"
     CDS             20219..20854
                     /gene="folE"
                     /locus_tag="PKI35_05865"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNX43946.1"
                     /translation="MKVENNMDDYPVQDGYEKRDLYNQKTIGELAGHYKEILRLVGED
                     PGREGLLKTPERVSKAMLFLTHGYDLNPEEMLRSAMFEEDYKQMVIVKDIEIYSMCEH
                     HMIPFFGKAHVAYIPNGHIVGLSKIPRVVDAFARRLQVQERLTTQIKECIQNTLKPLG
                     VAVVIEAQHMCMSMRGIQKQNSVTTTSDFTGAFLIDKTRAEFLHLLGSKLH"
     gene            complement(20936..22678)
                     /locus_tag="PKI35_05870"
     CDS             complement(20936..22678)
                     /locus_tag="PKI35_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43947.1"
                     /translation="MPKFKKVSLSFLVVLIYIIVIPIVDFNHQQYKGKEGVVVWDIKS
                     YYAYLPAAFIQKDLTLKFYEKDIQTWGKWIWPIETPTGKKAILTTMGMSCMYSPFFLI
                     AHEYAKISGKYAADGYSFPYHVALTFSAWVYFILGLIFLRKLLLRYFSDTATAFTILI
                     IGFGTNLFYYVTYEAPMSHAYSFALISIFIYLVDQWRENSYNWKYSILLGLLAGLITL
                     IRPTNAVALLLIPLFGIAAMKDFGKQVPVLLRQWPKIIVMALLCILVWVPQFLYWHEV
                     SGQFLFFSYGEDGSAFYFNHPRLLNILFSYRKGWFIYTPVMLFAATGIVFLFKQKRPF
                     SFAVLIFFLVNLFILSSWWSWWFGGGYSNRAFIDSYGLMALPLAALIDEFRTKKIAKA
                     LVITFLLILTSFNLFQIQQYRHQAIHYYWMNKEAYWEDFLKSRPTCKYFNILNQPDVL
                     KARTGIYEAVPIEDDKISKDDLFSFLEAELKDKKPVTDSLQEVSQRKGQPYEQVTTNY
                     INYIIYSGQAKAEYKALRIAYLKSQMEGCKSWRKEIERKALKNNLTFDRMASLEAERI
                     FNAYSQKYIKKSVR"
     gene            complement(23028..24479)
                     /locus_tag="PKI35_05875"
     CDS             complement(23028..24479)
                     /locus_tag="PKI35_05875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNX43948.1"
                     /translation="MKSMHSKQFYYDLLIPILLLAINLIIKALFLGNGSLGGDEPFTV
                     FYSQADFSTFAAMMQDENNPPLYFILLHFWIKLFGISVVSVRILPLIFSSLTVVFIYK
                     LGQKFLNVRVALLASLLFTFSNFHTFFAHETRVYPLFTLLTTISMYSFLSLAGNKGGR
                     KHFISLLFSNTLLIYSHFLGFFVILAQVIAVVFIRDFRKAILKKYCFICLLTFVLYLP
                     YLRLLISRFSTFNDKGTWVPSPTPEDLYTNLWKFSNAPVNTVVFILLMVTALILLILN
                     RKKISGIPPGNIKVILIWFLVPYLLIFLISLVTPMFLDRYLIFISIGYYLVIAVSIHF
                     IGSGKNWIFYPLSVMAVFLMLFTYNPRTGDERNTQELVNTIRQWKTGKTAVYLCPEWI
                     DKGFVYYYNPEYFKDYKNTRTRLNTDHVFPINSAHQVNDTLLLNCESAIYLDGWSVQV
                     DPENMIYRKLAGAFRKVECNENFHGYKIYHFSR"
     gene            complement(24716..25498)
                     /locus_tag="PKI35_05880"
     CDS             complement(24716..25498)
                     /locus_tag="PKI35_05880"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNX43949.1"
                     /translation="MNMRRSRHRFLKSAKLPAAFVLAFVLFSLTSCKKDHIFDFLKST
                     GEEVTVAREVTGNFTAIRIEDDVDLNIIQGDEYKIELQGGENILPGIVTEISDSTLAI
                     RNENTYNWVRSYDRKLIANVTLPHLLKLDYLSTGTVTCADTIREDSLFIDARGGSGYI
                     NLIVKTKLSHMSIHTGSADMNISGISNVNFICANGYGPFHCQDLKTVYTYMSTLSTND
                     CYINVNYYLEYKIMNRGNIYYKGHPEQIYGAKKGSGNLIPLD"
     gene            complement(25550..26728)
                     /locus_tag="PKI35_05885"
     CDS             complement(25550..26728)
                     /locus_tag="PKI35_05885"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HNX43950.1"
                     /translation="MTSVKKSKVRNHPPSHKAFIFIALTLLATPSVSKAQHKLINSNL
                     LLEGRVNYGFLINHHLEMKIFNSHFPAFEINLGKETFGQQRWESEYGYPIIGISYWYS
                     QLGNSEFLGRSNALFPYINYPIVRDVKHELNFRVGLGLGYLTKCFDPVTNYKYLAIGS
                     HVNAAINLMVEYRWRMSPRVQLASGLALMHFSNGSTKTPNYGINSPSINVALAYRLSN
                     ENAYQNRKLLPELYKFEFDGKRSVNLNLAATMGYKDMGQEYGKTFMVYNFSGDLLAPL
                     SFKSSGGLGFDFTRNQSDPYFAELQDVMIKHDYELYRMGISLAYQLRMSKLSYMFNTA
                     MYVFGTMAPSFGYFKFGLQYLAYKNVFATLTFRSHLARADFIGLGLGYRLPLYVYKPH
                     "
     assembly_gap    26967..27066
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            27303..28073
                     /gene="mazG"
                     /locus_tag="PKI35_05890"
     CDS             27303..28073
                     /gene="mazG"
                     /locus_tag="PKI35_05890"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNX43951.1"
                     /translation="MDSRLSEFTRLLDIMDELRAKCPWDKEQTFDSLRHLTIEETYEL
                     SDAILEKDLTEIRKELGDLMLHIVFYSKLGEETGSFGIADVLKGINEKLIRRHPHIFG
                     DVKVTSAEDVKDNWERIKLNEGRTSVLEGVPMSLPAMVKAYRIQDKARGVGFDWENRD
                     QVWEKVEEEIGELNHELANGNTPEKREAEFGDLLFALINYSRFIDVNPETALERTNRK
                     FIRRFQFIEQEAAKQGRKLHEMTLSEMDEIWNQAKNLE"
     gene            28096..29103
                     /locus_tag="PKI35_05895"
     CDS             28096..29103
                     /locus_tag="PKI35_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX43952.1"
                     /translation="MRRAFFPILLFLIFSGSSFGQSMRIHGIVTDSEGVPIPDAWIQD
                     NGRVGYLTITDSSGYYSVIINFTETSGEMLFVTCSSFGYQDIKVLIDTAFQNLVLYPK
                     MGIHTNSLHRNYPASLAGRYYGYDPNRINGSIDYYFEFNHADFSGYRDLLDSTNITSL
                     GKLSATKFDVSLMLQNYMLRIGYGSGSDLTIDEDSVSRRYKLSLAGIELGFSIPIGSH
                     IMINYLGGICNYRYRMVNKFSNGDVSLTNYLKKPEIDLRFNQPFVSNRLELGYRTNCP
                     VTESPVDFYIAFFTGFNSSISRSTLVCSRNSRLLSDQSIGFDRFIYGLKMAVIIKNRI
                     H"
     gene            29208..29837
                     /locus_tag="PKI35_05900"
     CDS             29208..29837
                     /locus_tag="PKI35_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43953.1"
                     /translation="MLKILPFLTLILILFGCQPSVTFTEPQPKGAKHLDRIPSKLSGH
                     YLSEDKASFIVISDRLIVQTYDYDLKTAKTDLLPHQKLVGDSLENTETGSRDFVMVTG
                     DSLVQHIHETDTLFLLSDGQVLTKFKGYYFLNTKWDQNWYVRQLSLSKGVLKLAEIST
                     SEEIDNLRQITGTEEDSTIYNFSPGKEEFRNFINQDGFHKKSRFYRISR"
     gene            29794..30783
                     /locus_tag="PKI35_05905"
     CDS             29794..30783
                     /locus_tag="PKI35_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNX43954.1"
                     /translation="MDFTKSQDFTGYRADRCPQDHLPGGSGLRGAHSPREWLTILRKV
                     GGAPVILLFVSFFLSSGISEAQTADSSTSGYTVINGPYLKSYVTDTRSFVKSPLQWKT
                     KQWLTMGAVAGAGVLLYSKDPEIRDFVREHHTAAGDDFFRYGFEPWGSGLYPVPLLGC
                     MYLTGRLAHNDRISATALTAGKAALISALFVNITKQLMHRHRPYQDNPANYAGWHGPF
                     SDWRYASFPSGHSAFAFSIAAVLASEYRETGWVPVAAYAVAAGTAFSRIYRDKHWASD
                     VFAGSALGIVTGRFLWKKNRTVRITPVLSAHYSMISVEVPLGCRHSRGDARCE"
     gene            30901..>32037
                     /locus_tag="PKI35_05910"
     CDS             30901..>32037
                     /locus_tag="PKI35_05910"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNX43955.1"
                     /translation="MKHKILLFLFAGLLVSFNTQAQDFWKLIPFPDSLGISAVTFNSQ
                     GDIYVTTGTDDDSIDDGVLRSSDQGQTWEVLYNNSYFAVGPIAVNDSGHIYIGVTHRQ
                     YGLRASYDNGNSWSIIQHPALGELTKIICVSTDTILVGAAKSGGVLLMLTPDRCITWD
                     TLFSTSNHTSETISDIAIAPDGTIYIGLMCFMEDMGGVYKSTDNGITWEYVGFHNNQV
                     SNIELNAEGDLYVGVFGSFTDLNGGLYVIRNGSATIDTCLYGPSINGLVVNTAGHIYA
                     SIAWTTGIFISKDDGETFEIESSGLPIGPMGRLYVDQDEYVYAKTDFSSSLLYRTVEP
                     TITDVKNKTELSSPNLLVLYPNPATDRLTGRLTRKGNCDGRYRLS"
BASE COUNT         8550 a         7109 c         7506 g         8772 t
ORIGIN      
        1 gaacgcaaaa cgtagaactc aaaactcaaa actcaaacgg ctaacggcct gcctgcctgg
       61 tcgaggtcgt gggcaaggcg gcaggctaaa agctaaaagc caaagggagc acggagaacg
      121 gaggtaaatg tcatggagca aggggcgagg agcagggagc caaaagccaa aagctaagag
      181 ccttgagtaa gactgcgaga ggatggcagg ggagagggca gagcgcgaga cgcagaacgt
      241 acagtctcaa ctgatcatgg accagtcggc gtttttgcgg cttaagtatt tcagttgctc
      301 tgccagggga cggtttttaa tttgttccag cgaagggtct tcgttcagaa ggtttgaagc
      361 aacccggcgc gcatatcgga gtatggcttc atcgcggatg atatcggcca gtttcaggtc
      421 gaggacaccg ctctggcggg ttccttccag gtcgccgggg ccgcgaagtt tcaggtccac
      481 attggcaatc tcgaagccgt tgctggtccg gaccatggtt tccatgcggg tccgggcttc
      541 cggtccaagg ttatatccgg ttagcaggat gcaatgcgat tgatcagcac cgcggcccac
      601 cctgccacgc aactggtgca actgcgataa cccgaagcgt tcggcgcttt cgatcaccat
      661 caccgaggca ttgggaatat caacgccaac ttctattacc gttgtcgcca cgagtatgtt
      721 ggtatcgcct ttcgaaaacc gttgcatttc agcatccttt tcatccggtt tcatccggcc
      781 gtgcactatg ctcaccctga acgccggagg cggaaaggct tccacaatat gctggtatcc
      841 gtcttccagg tttttcatat ccagggtttc cgattcctga atcaaaggaa acactacata
      901 tatctgcttc cctgatttta tctgttgcag catgaagttg tacacctttt cacggcctgt
      961 ttcaaagaaa tgataggtct tgatcggttt tcgtccgggg ggcaactcat cgattaccga
     1021 aaaatccagg tcgccgtaca gggtcatggc tagggtgcgg gggattggag tagcggtcat
     1081 gaccagaata tgcggaggat ccgcgctttt actccagagt ttggcgcgct gggctacccc
     1141 gaaacggtgt tgttcgtcga tcaccacgag gcccaggttg ctaaactgga ccgtatcttc
     1201 aataagcgca tgcgtgccaa tcaggatgtg caattctccg ttccggagga gcgaaaacat
     1261 ttccttccgc tgcttctgac gggtactgcc ggtaagcagg tcacaggtaa tgcctgtgcc
     1321 atccagcaat ttgctgatgg ttttaaaatg ctggttggcg agaatttcgg tcggcgccat
     1381 gaggcatgcc tgataaccgt tgtccagcgc tatcagcata ctcatgagag caaccacagt
     1441 cttccccgac ccgacatcgc cctgcagtaa acggttcatt tgtttaccgc tgcccatatc
     1501 ggcccttatc tccttcaata ccttcttctg ggcgcccgtg agagcaaacg gcaaatattg
     1561 cgaataaaaa gtattgaaat tttttccgat tattgcgaat ctgtgtcctt tcacggtacg
     1621 cgaacggaac aatttgtatc gtaacagccg caactgaata aagaacaatt cctcgaattt
     1681 tatccgtgct tctgcctttg ccagcatcgt atggtcggac gggaaatgaa tgttggctaa
     1741 cgcttcgtca cggggaatca gtttcagttc atccatgatt tcacgggtca gggtctcagg
     1801 gatcattccg tgtacctgca aaacaagcgt cttggccagt ttagccatcg cgcggctggt
     1861 aattccgtgc aactttgact tttcggttgt gctatagagc ggatgaagag ggtcggcagg
     1921 ctgggcataa aattcggcag ccgtttctac ttccggatgc ggtatattgt agcggccgtt
     1981 atagagcgac ggcttcccga aaatgacata ttccgtgccg gactgaaatt tgtctttaac
     2041 ccatttaata ccctgaaaat aaacaagatc aatagatccc gtttcatcgg caacagtaac
     2101 gatatatcgc attgatacgc cttttccggc tagttgcgtg ccggtcactc tccctttaag
     2161 ctggacccag gcggcgtcgc tgttaatttc gccgatcgaa aaaaaccggc ttctgtcaac
     2221 ataacggaaa gggtagtagg taagcaggtc atagaaagta ctgatcccga attccgaacg
     2281 cataatttca gcccttttgg ggccgacgcc tttcaggaat tcaataggag tatcaagaat
     2341 atcaaccgtc atttatctta aggaactttt tccgcatggg taacaaagtt aacaaaacaa
     2401 aacagctccc caaacggaga gctgtcggta attgggttta tctcagcaag tgattagtat
     2461 tcccacaatt gctgttcaaa attaaacagg tcttccttga tgcgttcagc ttcgagctgt
     2521 gcgtcaatac cggtagcgta ttcagcgatt ttacggtcgt aaacgttttc ttctttgtag
     2581 atgtagctgt taaacatacg tttccagaac aggtcatcat aagtcagccg tgcagcattg
     2641 ttgaacctgt taaatacttc agcttttgca aaaacggcac gggcttcggg gaagtagatc
     2701 cagaagagcg gttcataagc gcggaagttt ccgttttcat cgtagttgtc gcgtacgggg
     2761 caaatgccca gtatccttac ttcaaacatg gaccgttgtt tatcgaaata ccagtcttct
     2821 ttgatcctga accgtttgac atcgaggggg ttgaactttt tcgacattac cgtatcatac
     2881 cagtcataag ggggataagg gcgttgcatg ggaacggtat cggtccgttc gaggtttgcc
     2941 atcaactcct tgtaggtaag agggacgatg aattcatcgg ttggcgaagc ggcatcgtaa
     3001 gcggtaatgc tgccttcttt aagggcatcc ataataacag tcatgaaatt cttccagtta
     3061 ttatgcggcg tttcgggata gtaaaaggtc tgattgattt tttcgcggac atcaaccata
     3121 cgccagatac gttttttcca tacaaaatcg gcataccgca cccaaggata cgggataggg
     3181 ttcttttcga gcctatctcc ttcatcaaca acgccgtcgc ggggaggtgt attgagcagt
     3241 tgctgggctg aggcgcctgt tgctataaaa aacaccagga gagccattat taagacagat
     3301 ttcttcatta ctagggtgtc tgaagattta ttttattgaa gggtaattat gatggagttg
     3361 agtttacggg tgccatcggg tcctttggca tatatatcct cgaaccagat tttatcacct
     3421 tttttacaac cacgtatgaa gtctctcatt tcttcggtaa gcctgttgcc acgggccgga
     3481 cgagaccaga agtcgccgcg gcgcactccg gaacaagtga agccggtaac ttcaaaagta
     3541 aggtcgaagt cgaaatcggc aggcattcgg ggaataaggg ccgaagcaag taccatttgc
     3601 tgcgatgccg ggccgcctga tgtatttgca atataagcgg taggatcggg tacacgtttc
     3661 accctgaatt cggcagcgcc aagcgatttt gcgcggtcgc ccattctggc agagacactg
     3721 atgacggctt tgccggtttc attgacgcgt acgttccatc ctcttcctga tctggtaata
     3781 gagccgttgg agatggaagc ctgtaactgg gcatcggata caccgccggc cgaaatggag
     3841 accgggttat caacggctgc ataaaataca ttcatcttgg taggggaaac agtcaatgta
     3901 ggaggggcta caatgtagtc aacggctaca ggatagtttt gcatttcgcc cgaaggggat
     3961 ttcacattta caaatccttt cagcgataca ggacccggag tagtagcggg aatggaaatt
     4021 tttgccactc cttcggtgct cgaaacccca cgcccgccaa tgttggccgt aaaggtttgc
     4081 ttggtatcgt atgcggtgac aaaaatttcg gcttcgtagt tttcacccag gaaaacatat
     4141 tgactttttg gaacaacagc agcgccgaca gcatcaaatt tatagtcgct ggcggttatc
     4201 tccgacagaa ggtgtgtaac cacatcctgt tcggcgttca atacttcagc tacaattttg
     4261 ttcatgatgg ttacatcggc agccagaatg gtatggtaga agttgtacat ttcccaattc
     4321 tgctttgctc cgcttgcatc atagtaaggg ccatcggtat caagacccag ctttatctgg
     4381 ttacgcttgg caggttctat catctgcagc atatcctgct tgtacttgat tattttatct
     4441 ttcaactgac ggctgcggcc tttcgacccg tcctgtgagt tgccgataaa gaaatttgtt
     4501 gtcttgtcgt atttatcctt actggcaagt tggcgaaggg gcgttgtgcg cgctgcatcc
     4561 atcgaaattc cgtcggaaaa agctattgct tcaaacttgg tattttcgac ataatctctc
     4621 atttcctgcg aaagtttttt cactttcatg gccttatcga aataagggcc gacttttgca
     4681 gggttctgca tgttttggat ctcaaactga cgatataggt tctcggtttt cgcacggaat
     4741 acgcggttag tggtttccat gctttcgttt acaacgagaa atgcattcaa tatctccacc
     4801 gatacattga gagccagcat agcagtcaac accaggtaca tcatcgcgat catcttttgc
     4861 ctcggtgtct ctttatatcc agccataatt cagggttttc aaagtttaac gattttatta
     4921 tatgaggttg cgatgagtac gctatttatt cggattaaca ttcatggcag ccagcatatt
     4981 gccgtatacc tgattgaggg cggtaaccct tttggtaagc atttcgacct cttctttgta
     5041 tttatccatg ttgcccgagg tttcattgat gcttccgagg aacgaattga aggaagcgcg
     5101 cagtttattt ccgacttcga gttgttcgtt cgagctctgg agttgcattt catacatggc
     5161 attaagggcc gagaggttgt gggaaatctt ctgcaactgg tcgttgtatg atttcgaatc
     5221 gagtgccgtg aagttgagcg attcagccga ttttgaaagc aattcggcag ctttgttata
     5281 tttttcggtg agccggttgg ccgattccga agcaacagcc atggtattgg agaactgttc
     5341 ggtggcgctg agctctttct ttatcgaatt tgaaacatcg ttgtaagtag tggccaatgt
     5401 gccggctgat gtggcagccg atttaacgct gcttgaaaat tcaccgaccg aattggcatc
     5461 ctggttcaga gcatcagccg tctttttata cgaaacggcc agttcaccag ctgacttgct
     5521 tgcatcctga acggttttaa cgaattcgct gttagcggcg gcggcattgg tgacatccga
     5581 cattttagcg acattttcgc tcatttttcg catgccgttg cccaggcttt caataagttc
     5641 ggggcctatc tttgccttcg acaacatatc atccagttcc tgggtggtcg atttggtggc
     5701 ggtatgtact ttatgcggag cagcgcttgg cgttcctgca ccaccatgat acattcctgc
     5761 aagttccgga tatacaaggc tccagtcggg ttcaacgtgg ggtggttcga aagctgagaa
     5821 aaagaaaatg atagcttcgg tacacatacc cagaatcaac atctcgttgg ctcccgtata
     5881 gtgattaatt ttaaagagtg cgccaaggat aacgagggat gcgccccagc catacaattt
     5941 cgacatgaaa tttttgtatc ccttactgct aacaaaactt gttaaaccca tagtattgtt
     6001 gttttatttg tttttattca cgttattttt tgctcgtata ttgatgagtt ttttcgtaaa
     6061 aggtcatacg aaattttgca ggttatttta attatagatg cgtgaggccc tggaggggtt
     6121 atcatccttc tgcctgccca tgaaatcctg cacacaacgg aaaccgatgt agcatttgga
     6181 agaatcctga tattcatagg cgcgcgttga aacctggatg taatacgata tgtccttcca
     6241 tgaacctccg cggataactt tacgtttcag ggcgggagga tcgtcttctt tgctgttgta
     6301 ttgataggta gggttcatat cccaggtgaa attggaggat gattcgtccc atgcgtcgga
     6361 acaccattcc gatacgttgc cagccatgtc atacaatcca aaatcattgg ctggataatg
     6421 ggctacgatt accggagtag ctccgccgtc atcaatgtaa ttaccgcgca atggcttgaa
     6481 gttggccagg aaacagcctt tatcgtttcg ggtgtaaggg ccgccccagg gataggcgct
     6541 gaagctgttg ccgccacggg ctgcccattc ccattcagcc tcggtaggaa gcctgaattc
     6601 gctgagcgaa gcattgtttt tacgtgccag gtaagaatcc atcagacggg tgcgccagat
     6661 gcagaaagca gaggcctgtt tccagtttac gccgacaaca ggataattat cataaaccgg
     6721 gtgccagaaa tactgctcaa ccatagggtc attataacta taggcatagt catgcagcca
     6781 tgccagggta tcgggataaa cgttgacggt ttccttgcgg atataaaccg aacggtcttt
     6841 catgccctgg ggacggttgg cgaaaccggc gtttttcgaa tcggtttgac ctgaataatc
     6901 tttacgtgat gctgctttta aatcaatgaa gtagtactcg taaaaaagct tacgggtgtc
     6961 aatttctttt tttcggaaat agcgctcgtg ttcaggaaga tacatggatt ccagttgatc
     7021 gcggtattcc tcggactgcc attcgatctc ggtttcccag ttcaggaagg gcgggtcata
     7081 ggtttcgccg gttttcttgt tttccgaaat caggaagtct tctgcgttta catcaccaag
     7141 gatacgacgg gcaatggaat cacggaccca gtacacgaac tgccggtact cattattggt
     7201 gatttccgtt tcgtccatcc aaaaagcctt tacagtaatg gttcgcggcg tattcagatg
     7261 tgcatacggg acgtcctggt cgccggggcc catcgtatat gagcccatag ggacataagt
     7321 cattccgaac ggatcgggtt gataaaacct ttccctatcc tgcactccaa taagttctcc
     7381 attgccggtg ttacggcaac ttcctaaaac taatgccacg agtaaaaata aaagcagttt
     7441 tttcattgta ggttggtttt gtttgattct attgccatca cggtcatact gcataggttt
     7501 gttcctacag atggcaaatt atcaaaagcc tgaatgaagg ttaatcactt ttccagcgct
     7561 ttgtaataat cagacagaat cattgcagta tgcaaataac ggtttttacg ttgctttatt
     7621 attttattcc cgtattttcc gaatgcaaat gtatgaaaaa caccttcatt cataaaaatt
     7681 acaggtatcg ggtattacgg tagcctgatc ttgatttttc acgttcaatc ctgaatatat
     7741 accgtaacat tatttcatga ctgcctgtgg cgcctacccg gctggtaggt atgtcatagg
     7801 cgtatccaat ttcaagttcc ttggcattca gtcctaccat gagggctata gcatcagata
     7861 gcctgtaaga aacgcctccc catacttttt tattgtatgt gaccattccg ttaaaatcag
     7921 cctgcagggt agatccatcg gattttacaa aaactgatgg cgttaatatg aaagccggaa
     7981 aggctggaga tgcaaggtta atgccgcctc cggcataata atgccttctt agggtaaacg
     8041 ttcccacgcc ttccgctccg ccaagatctt tttccgattg aagcagttga gtcgacgaaa
     8101 gtgaaaggta gtatttggga ttattgtaga aaatgcctgc cgacatatcc gtgattacaa
     8161 ctccttgtga tgatttcccg cttagctggg ggtcgccttc atcaaccccg tgtagtttat
     8221 tcatgtcaag ggctttgttg agtaactggg cgttcacgcc aattcccagc actccgttgc
     8281 cgacagtaag atgataggca tatcccaact ttacagccat atcggtatag aaaccatact
     8341 tgtcctggac aatattcagg cctattcccc cgtgaagaaa tttaatcggg gaatcggcgc
     8401 caattaataa agtttgcgga ttgactttgt tgccgtcctc gtctttaaac ccaagccatt
     8461 gttcgcgtaa aattcccgta acgcatattc cttccgataa tccgtaatgg ccgggattca
     8521 catcagaata gttgaacatg ttatgcgtaa actgaggctc ctgctgagca taaatttcag
     8581 caaagggaca gttgatcatc aacagcacaa caactgtgaa gcgcagaatt acaggtattt
     8641 tttcttggag cggcacaata cttaaaaaac gacgctaaag tagtaatttt ttattctctg
     8701 gttcattgat tttcaagaat cttgaaggtt ttacccaatt atattctatt tattactgac
     8761 attcagtaaa gtaatctggc ggaacccgga ttcatgtggt aagcaaactg acctttaacg
     8821 ccagaatcgc ttgagtagta tagttctcat acaaaattaa cttttttcac ttttttttct
     8881 ttttaagtaa ttacttttta tcaactggac tataaatata aaatgaaata tttgatattt
     8941 gccaaaaaaa ctcatcaggc aaacaaacca gaaatcaata ctcgtaaact tatgaaaatc
     9001 gaccgcctca gaaacatcat tttattttgt gcagggcttc ttattttagc atcatgttca
     9061 aagtcaaaca gccaggaatc tgccgtttcg caaccggcgc aggttgaaca gcccgctgag
     9121 aagccgggaa aagttccgca gattactgag caggatatgg caaaactcaa tcagtttgat
     9181 ccctcaaaag tttcttcgga tattgtttta aaaaacagcg ctgaagtata caccagcgat
     9241 aacagcaggg catttgcttt caacgaagtt cagaagaatg ctgttagctg ccttatctac
     9301 ggaggggttt cggaagataa aaaatgccca aagtggttca gtcagaaaat attacaatgg
     9361 gacgcagcta caaagcaatg gaaaacctac tttgacagcg gtataaaata taattccgtt
     9421 atagcggaag atcataaagt agcagccggt tcctggtact ataccattca atgggtatgg
     9481 gaccccaccg ccaacgattg gattaaattt gatgccgatc tgctctttaa cgcggtaaat
     9541 ggttagtgga agctttggtt aggcttgtgt taataatgag aagggagagt taaaagctct
     9601 cccttttttt tcttatcttt ttaatgacag acaattttcc gaactttgca cctcgctaaa
     9661 gcgcaggcgc taacgcgctg taatacaatc atataaactt aaatcatgat gttttcagga
     9721 aacgcaaaac ttttttcaaa acgttggttc attgattatg ggctgataac gctaggttct
     9781 tttatccttg cagcaggttt cgtatttttc atctcgcctt accggattgt tcccggaggc
     9841 gtctacggca tttccattgt tatccactat attacgaaag ggatattttc ctgggcgccg
     9901 gaaggatttc ctatcgggct gatggggctg atccttaata ttccgctaac cattattggt
     9961 ataaaggtgc tgggcccgaa attcggtgtg aaaacagttg tgggatttgt actctcatcg
    10021 gtttttatgg atggcatcac catgttttgg ggtgaaaggc cattggttga aggcgacgca
    10081 ctcctttctt ccatttttgg cggagttctc atcggcgccg ggctcggact cattttcaaa
    10141 tcgaaagcca catcgggcgg ttcggatatc atcgccatga tcattgccaa atacaccaac
    10201 attcccctgg gacaattaat gatttatgta gactcaacga tcgttctgct tggactggta
    10261 gtatttgccg actggaaaat tcctttatat tcgtggatcg ttatttatgt taccggaaag
    10321 accattgacg tggtaattca gggaatgagt gtcgataaaa cgctgtttat catttccgac
    10381 aaatttgaag aaatccgtga cagcattatc aatgacttac agcgcggcgg cacctatatt
    10441 cccggatcgg gcatgtataa cagagccagt aaaaccatta tcttcacggt tgtaaaccgc
    10501 cgcgaaatgg ctatccttga ggaatatatc cacaaggttg acccgaaagc ctttgttacc
    10561 gtactcgaag ccaatgagat tctgggcgag ggatttaagt cgctgaggga taagatttca
    10621 gattaaagca taggccggca tcatcgttgg tggtgaaaaa agacaaacgt gcgattgttt
    10681 gtaatgtggg aggttagaag ctgctggttt taagataccg gctgattaca gatgctccgt
    10741 ttccggcttc cggcctgccg cctgcgttat ttgatatgcg tgaacaataa gttcaaatcc
    10801 ttcaccgggc aatcatgtta ccggtgaact gtccggggaa tgtcaatacc ataaaactta
    10861 gaaaaatgat aaaaagtccg agttttctgc aacctgaaaa aatattttct gcaaactggt
    10921 tccgctcata ttcctatatt gtgatcggta ccttcatcat ggcgtgtgga tatgtcctgt
    10981 ttatcagtcc cgaaaagata gttccgggcg gagtttacgg gatagccatc atgcttcacc
    11041 atttgctggg acttccaatc ggactcacgg cgctggcttt taatattccg ctgaccatca
    11101 tcggcacccg gatactcggg ccgcgtttcg gcgtaaaaac agtcgtcgcc ttcgtcctga
    11161 cttccgtatt tgttgatctg ttgtcgtatt ttcacggaaa tgcaccgctt gtggaaggcg
    11221 aaacactggt atcgggaatt ttcggcggat tgctggtagg catcggcgtg gcgcttacgt
    11281 tcaaggcaaa agccaccagc ggcggcagcg acgttgttgc catgattctt gctaaataca
    11341 ctcaccggcc gctcgggcaa ctgatgatga tcgttgattc gtcaatcgtc ttgttgagct
    11401 tccttgcctt tggtgattgg cgaattcccc tttttagctg gatcacaatt tttgtcattg
    11461 gcagggtagt cgatactgtc atgcatggat tttcttacga caaaaccttg ttcattgtat
    11521 cggaaaaata caaccagatt cgcgacagta ttatcaccga cctgcatcgc ggcggtacct
    11581 ttctgcatgg tgaaggcatg tataaccagg cacagaaaca gataattttt actgtggtca
    11641 atccgaggga attgtcgatg ctacaggaat atatacaccg gattgatccg gatgcttttg
    11701 ttgccgttct cgacgcctat gaaattctcg ggaaaggttt taaatcgctc tccgaaaaag
    11761 tggaagatca gtcgtaaacg cggatacgtt tacctgaaca atcggatgat gtcgttgccg
    11821 ttggctaaaa tcagaagtcc gaagagcagg accatgccca caatctgagc atattccagg
    11881 aatttgtcac tgggtttgcg cccggtgatg atctcaacga ggacaaacag tacatgtccg
    11941 ccatccaacg ccggaattgg cagtatattc aggattgcca gcatgatcga caggaaggcc
    12001 gtcaaagccc agaacgattc ccagtcccag gttgcaggga aaattttacc gatcgtgata
    12061 aagcctccca ccgattcata tgccttaacg tcttttgaga agatcagttt caactgtttc
    12121 cagtaattat cgatgccttt ccaggttttg acaaagcctg ccggaatagc ctgaaatgca
    12181 ttataatgcc tttcgctgag gttgaaataa gtgaccggtg gtttgggggc cacgccgatc
    12241 agtccctttt ccgggacttt tacacggatt ctgaccgtat cgttctcccg taaggcaact
    12301 acagttactt cctggttttt atgtttttgt atttcggccc ggaacatgtc gaaatatggc
    12361 gtgctcagtg tatcaatgcc gataatttta tcgccttttt tgaggcctgc agctttggca
    12421 ggcgacgatt tggcaaaatc atccacttcg aaaggaatcc tgacgctgat aaaatcggcg
    12481 gactggcttt tgatcatctt tgaaagaaat ccttcaggaa tcacgacttc agcaggtttg
    12541 ccgtcacgtt caacctggat ggtccgcgct ttctcgagca ggatatggct gggtatcttc
    12601 gaaaaatctt ccacaacagc attgtcgatg gagatgattt tatcgccatt acgaagcccc
    12661 agttcatatc cgagtgaatc gacggcgata ccgtatttca cttcagaagt cggaagatat
    12721 tgttcgcccc atattgccag cgtggcaata tagatgccga tagccaggac tatattcatg
    12781 ataacgccgg ccaccatgat aatgagtctc tgccatgccg gcttggcgcg aaattcccac
    12841 ggttgggccg gttgcttcat ctgctcggta tccatcgatt catctatcat tcctgaaatc
    12901 tttgtataac cgcctaaggg cagccagcca attccgtatt ccgtatcccc ttttttaaac
    12961 ttgaaaatcg aaaaccaggg atcgaaaaag aggtaaaatt tttctacccg cgctttacaa
    13021 gccttggcgg acaaaaaatg gcccaattca tgaatgaata cgaggatcgt aaggctaaat
    13081 agaagttgga ctgttttgat gagaatttcc ataggttatg ttgggttctt cggggtgtaa
    13141 actggacgaa aaaagttttc gaatgtcatc tggcaagtga ttttatcaat tcctgggcca
    13201 caagccgtgt ctcggtatcc gttgcaacaa aatcaccgaa ggatggcgct gcaacaaagc
    13261 tgattttttc catgcaactc tctatcagat ctggaatctg taaaaatcct atctcatcgc
    13321 gaaggaaggc atttacggct acctcgtcgg cagcgttcat gatgcaggcc atgttcccgc
    13381 cttttgccag ggcttcataa gcgacaggca ggcaacggaa ggtatccgga tcaggatttt
    13441 cgaaagtgag ctgagggtat ttcgtaaaac tgaacctcgg aaaatcgctg gttaaacgcc
    13501 ggggatatcc gagggcgtat tgaatgggaa ggcgcatatc gggcaggccg agctgggctt
    13561 ttatggagcc gtcggtaaac tgaaccagcg aatggacaat actttgcgga tgaaccacta
    13621 cttcaatccg ggaaggtggc aatccgaaga gccattttgc ttcgattacc tccaatcctt
    13681 tattcataag agaagcggag tcaatggtca ctttatttcc catgctccag ttggggtgtt
    13741 tcagtgcttc gtctttggtt acggtagcga ggtaggaccg gtctttgccc ctgaaaggcc
    13801 ccccggaagc cgtaaggatg attttttcga cagggttatg aaattctcct gccaggcatt
    13861 ggaaaattgc cgaatgttcc gagtcgacag gataaatgtt gattcctttg ctttgggcca
    13921 aaccggtaac catttcacct gccacaacga gggtttcctt attggccagc gcaatggcct
    13981 tgccggcttt gagggcattt aaagtaggct tcagcccctg gaacccgaca agtgctacaa
    14041 gcaccatgtc aatggtttcc atttccacaa cctggcagat ggcctcatgc ccggcatata
    14101 cctttatatc gtacgggtcc agcgcctcaa acacacgctg atataaggat tccataccta
    14161 tgaccacggc attgggttta aattcaatgg cctggcggat cagcaatgcg gcgttggaat
    14221 gagcggtgag cacttccaca tcaagttcgc ggggatggct ccgtattaca tccaatgcct
    14281 gcgtaccaat ggacccggta gagccaagta cggcaatctg acgtttcatg gaagtggaat
    14341 tattgctcgg cacgggaatg atgtgtttgt aatcagaaga gaatataatc ggccgggttt
    14401 accggactgc cgtttatcca taattcaagg tgcagatgcg ggccggtgct taactctccc
    14461 gattcgccga taatggcaac ggtttcgccg gcacgtacta cgcttccttc tttcttcaat
    14521 aacaccgaat tgtgtttgta aaccgaaata atgttgccag ggtgcgagat tgcaagaaca
    14581 ttaccggttt cgaccgtcca tcccgaaaaa ataaccgttc catcctgaac ggctttgatg
    14641 ggttcatcgg tgttggcaac aatatcaatg ccgaaatgtt taagcaaagg attgaattta
    14701 ctgctgatta cacctttaac cggcgtaaag aaagtataac tccggctggt agcggaactc
    14761 ttttcagaag gggcatcaga ataaaacagg ttgtatttgc tctggttttc gtaatcaagc
    14821 ctgagtaacg agtcttcggg cgaccgtttg tcgaaaatat cggtatagct ctttttgatg
    14881 gcagtatcgg atttcgcttt tttctgtacg gataatgaat ctcccaacac cagcctcagg
    14941 ttctgaagat agagctggtt gctctccata gcgcgttcga tgctgtcgct gcgcatctgg
    15001 agctcataaa tacgccgctg catggctaca tcggtatatc cggggatata ttccctgaga
    15061 ggggtaaaag caataatgta agaggtgatg aaaaccagca cgatggaaag cagacctaac
    15121 acaacaaata cattcattcg ggttaatttg aatgacagct tttcttcaaa agtctcttca
    15181 ttcagtataa ccagccggta tttaacgcga aggcgttcct gcagtacctg ccagattttt
    15241 tcgccttctt cttttattcc actcataatt ccggaatcat gcggattaat ggtgcaaata
    15301 tcggaaaaaa caagcataac acgttcccaa tcactattaa gcctgtattt taagatttat
    15361 tatgcttttt cgaagaatgg aaccggatga acggtctgat ctgcaaaacg gccgttacgg
    15421 cggggaaaat gcaccgtatg cggagaatac ggctgaggcg caggtcggat aaaggaaggc
    15481 gtaaggctga aagataaacc ggagatgaag agaaatctgg ctcccggctg tatagacggg
    15541 gccggtatat tatgccgttt gaaccagcga cgggaatgtg tcgcgaaaaa cggaaaggaa
    15601 cccggaaccc tgctgccggc ggtaaacctt ctgcggctag ccttattggt tcttactgaa
    15661 aaataaaata tttttgcatt ctgagagtta ttagaatacc cataaggcac aaggcatcat
    15721 tcatttaaaa atgttatttt gaaatttcgt actctttccc gatatattct gatcccggct
    15781 ttatttcttg gcctggcgct gacgttttca tgcagcacca agaagaatac ctttacccgc
    15841 cgggcgttcc acaacgtaaa tacacactat aacgtgtact ggaatggaaa tgaaagcttt
    15901 aaaacgggag tgacggagct tcagtccgct gtgcgcgaca attataataa agtattgccg
    15961 gtattcaatt acggcacgcc tgaagaggcg aaaaaggtga ccggtcgtat ggacagggcc
    16021 atagaaaagg ctacgatagg catacgcagg cactccatgg tttttggtaa taaggaatat
    16081 aacagatgga ttgatgactg cttcatgctg atcggcaaag cgcagtttta caagcaggat
    16141 tacagtacgg caatccgtac ttttgagcat ataatgtcgg aataccaggg caagcccgtc
    16201 tattatgaag ctttgctctg gatagcccag gccaatatcc agcaaaagag atacgaagaa
    16261 gcgcttgtcc gccttgatga acttgatgga ctgctcacca gggaaacggt gccattccgt
    16321 atacgcaaga cgttaccgct tgtatatgcc gattattatc tgaggaccaa gaatgttaat
    16381 gcggcccgca atgccctgct gcaaggcatt gaattaaaca cgggcggtaa aaccagggtc
    16441 cggctgtatt atatcctcgg gcagttgtac cagcaggaaa agaacttcgc catgtcgacg
    16501 gagtatttca ccaaagcagc caaagggcct gcctctttcg aaatggcgtt caacgcgcga
    16561 attagcctgg ccaggtcgtt cgatgtttat tcaggcgata aggcaggtct tgaaaaagag
    16621 ttgcggaaaa tgctcaggga tggcaagaac aaggatttcc gcgaccagat atattatgcc
    16681 cttgcagaac tctcgcaaat tgatcagaat gatacgctga ccatgagtta cctgaagaag
    16741 tcggttgcaa ccagcacgga taatgatttt caaaaaacga cctctgcgct gaagcttgcc
    16801 ggtatgtact tcgacaggca gaattatgaa atggctcagt cttattacga taccacgctg
    16861 caggttatcc ccctggatta tcccgattat aacgctatca actcccgtac cgtcacatta
    16921 accgagctgg tgcagaatct gcaggtggta aaatatgaag acagtctgca gcgcctggcc
    16981 gggatgccgg atgaacaact caatgccatt attcaaaaga ttgttgaaga ctataaaaag
    17041 gaagaacaac gccgtaaaga agaggaagaa cttcagcaat cccagatggc ggcgcccacc
    17101 aatgccctga atatgcgtaa cgagaatatc cagaatatag gcggcggcgg atggtatttc
    17161 tataatccgt cggctatcag ctttggttac agcgaattta ttcgcaaatg ggggcgacgc
    17221 aagctggaag ataactggcg tttgagcaat aaaaggcccg tcgtatcggc ggatgaaacg
    17281 gaagtggcag aaaatgagcc ttctgattca gccagtaata ttgtggattc ggtaaaattt
    17341 gtgaagaaaa gtaccgaccc gaaagacccg aaaacttacc tccagcaact ccccaggact
    17401 cctgaagcgc tggctttgtc gaacaagcac atcgaagatg ccctgacgaa cctgggatat
    17461 atttacaagg acggcctgca ggataccctg aacagcatac agacttttga aaacctgatt
    17521 aaacgtttcc ccgacagcaa gaatgcccag cgtatttatt atcagttata tctcatctat
    17581 tcaggtattc ctgattcggc taatgcggaa aagtacaaaa ataccatact gaaccttttc
    17641 cccgggagcg aatacgcgcg taccttgcag gatcccgatt atttggcgca gatcctggcc
    17701 aggaaaaacc gggcgctgtc gctgtacgaa gaaacctacc aggcttttga acgagggcag
    17761 taccgcatgg tgatgcttta ttgcaatgaa gccatcagca gttacaaaga caaagacttg
    17821 cttccccgct tcgaatacct gcgggcgata tcgcagggga aaaccgtttc gattgataca
    17881 atgcttgtct cgctgaataa tattgtcgtt gactatcctt caagtcccat catcccgctc
    17941 gttcaggaag ttttacagaa atacagcaaa aagcagccgc cggcagcctc gccggcagcc
    18001 accgcacagg ccgcgacaac cggaaaccag cagggaagtg ctccagccca ggctccggct
    18061 tccgctccgg caacaaccgg atctatcttt acttccgata ctattgttcc ggatatctat
    18121 aggttcaatc ctaagcagac gcatttcttt atcatgatgg ttgaccagaa tacggttaat
    18181 attaatgcac tgaaggtccg tatagcggat ttcaacctga agagtttccc gaatgataac
    18241 attacggtga atgctattgt gcttgaaagc ggatggcaga tgctgacggt cagcagtttc
    18301 ggtaactcgg aaaaggccat gaattatttc aatacgatca tgcaaagcga ttatgtgctt
    18361 tcacagctta agactgccaa ttaccggagc tttgtgatat ccatggaaaa ttatcccgtt
    18421 ttctacaggg aaaagaaata ccagggttac attaatttct tcaataaata ctataaaggg
    18481 tagacctctg ctgatttctt ctttttaagc cgggcccctc aacaccggtt ccgccaaatt
    18541 ttccggtaca tttcattgta ttatcggatt aaaagttatt tttgctacaa agaaatattt
    18601 acaatggcaa ttactaaacc tactcttccc gaaacgcctt cggtaaacct gcttggcagc
    18661 ggcacagaaa ttaacggcga tatccgttcg aatggcgata ttcgcattga tggcagcctg
    18721 aacggtacga taaactgctc gggcaaagta gtaatcggct ccaccggccg gatcgagggc
    18781 gaactcatct gccagaatgc cgatatttcg ggcgaagtaa aggcaggcgt cagagtggcg
    18841 gaactgctga cccttaaagc cacggctgtc ctgtgcggcg atatcatcac caataaactc
    18901 gccattgaac ccggtgccgt tttcaccggt acctgcaaaa tggaaagtca gaattccgtc
    18961 aatggtaaaa caacggacga agcacaaacg gaaccagctt gaagaagggc gtaaacaatt
    19021 tgatggatat gcccgctatt cgaacctcgc cattcagatg tgtgtaatca ttggcgcagg
    19081 agtatttggc ggtgtagaaa ttgaccaatg gcttaagctg cgtttcccgg tttttacgtt
    19141 gattttaaca gtattttctt ttttagccgc aatctatatc gccataaagg attttatccg
    19201 aaagaaatga agacgacact cagccgcttt ttccgaaatt tgattgtttt tacactcgtc
    19261 gtggccgcag cctgcgcagc gctttttatc ttcattccgg cgctgtgctc gccaatgctt
    19321 ccctggttgc tggcattttt ctttgtgatc acattagccg ttttttatat ctccctgaaa
    19381 gcaagccgtg aaaaattaag ccggtttgta aatttttaca tggtgatgag catgctaaag
    19441 atattcatgc tgcttgtggt cacagcaatt tacctttatc tgagacgcga cgatgcgctt
    19501 cgcttttccg tttcgctcct ggttttattt ttcgcttacc tgatttttga agtcatctgg
    19561 ctcctgaaaa tacggggaga caactaatct tctgggcgtt tgctttttcc tggctgttaa
    19621 aactttttct ttattctttt gttgatcatt gaccgggatc aactcaaacc cgttttttag
    19681 ttaattttgc agttcaatcg ataaatcaat gatttatatc acccgtcgag agcgtttcaa
    19741 tgcagcccac cggcttttcc ggaccgattt ttcggatgaa agaaatgcgg aggtcttcgg
    19801 caaatgcagc aatcctttgt ggcacgggca taattatgaa ctctttgtga cgctaaaagg
    19861 cgagccggat cccgagaccg gtttcctggt taacctgaaa gccctgagca gcttaatcgg
    19921 gcaattgatt atcgagaagc ttgaccatca taatattaac ctggaagttg atttcatgaa
    19981 agataaactg gcctctgctg agaacctggc tatcgggata tggaatgaac tggcagcgcc
    20041 ggtccgcaac atgggcgccg agttgtattg tgttaagcta tgcgaaaccg aaaataattt
    20101 tgttgagtac ttcggaaact aaaaggcatg ttttcagcaa tttaagctga tgacaaacca
    20161 taaaacactt actacgcact attaactgac ccccatgagc agcgaaatca atgacccgat
    20221 gaaggttgaa aacaacatgg acgattatcc tgttcaggat ggttatgaga aacgcgactt
    20281 gtacaatcag aaaaccatcg gggaattagc cggccattat aaagaaattt taaggcttgt
    20341 cggcgaggac cccggccgcg aaggactgct aaaaaccccg gaacgggtat caaaggccat
    20401 gctgttcctt actcatggct acgatctgaa ccccgaggaa atgcttcgtt cggcgatgtt
    20461 tgaagaagat tacaaacaga tggtaattgt taaagacatt gaaatttatt cgatgtgcga
    20521 gcatcacatg atcccgtttt tcgggaaagc gcatgtggct tatatcccaa acggacatat
    20581 tgtgggactg agcaagattc cgcgcgtggt cgatgccttt gcgcgaaggc tgcaggtaca
    20641 ggaacgcctt accacccaaa tcaaggaatg catccagaac acgctgaagc cgttgggcgt
    20701 ggccgttgtg attgaagccc agcatatgtg catgagcatg aggggaattc agaaacagaa
    20761 ttcggtgacc accacctccg atttcaccgg cgcctttctg attgataaaa cccgcgccga
    20821 gtttctccac ctgctcggaa gcaaactgca ctgacgccat cgggcgtttt gacctggaac
    20881 tgacgctata cccgggaatg taacaaacag caatggcttc gagcaggcaa aatgcttacc
    20941 gaacgctttt tttaatgtac ttttgggagt aagcgttaaa tattctttcg gcttccaggg
    21001 acgccatacg gtcaaaagta aggttgtttt tcagcgcctt cctttcgatt tcctttcgcc
    21061 atgatttgca gccttccatc tgtgatttca ggtatgcgat gcgcaacgct ttatattccg
    21121 cttttgcctg tccgctgtaa atgatgtaat tgatataatt tgttgtgacc tgttcatacg
    21181 gctggccctt acgttgcgaa acttcctgaa ggctgtcggt taccggtttc ttatccttta
    21241 attccgcctc caggaagctg aataaatcgt ccttcgatat tttatcatcc tcaattggca
    21301 ccgcttcata gatgcccgtt cgcgctttca ggacatcagg ctggttgagt atattaaagt
    21361 atttgcaggt tggcctcgat ttgagaaagt cttcccaata ggcttctttg ttcatccagt
    21421 agtaatgaat ggcctggtga cggtattgct gtatctggaa caggttaaag gaagtcagta
    21481 tcagtaaaaa ggtaatgaca agggccttcg ctatcttttt agtccggaac tcatcaatca
    21541 gagcggcaag cggtaaagcc atcaatccgt aactatcaat aaaggccctg ttgctgtaac
    21601 ctccgccaaa ccaccagctc caccaggatg agagtataaa aaggttaacc agaaagaata
    21661 tcaggacggc aaaggagaaa ggccgttttt gtttaaacag aaaaacaatg ccggttgcgg
    21721 caaaaagcat cacaggagta taaataaacc accctttccg gtagctgaac aatatattga
    21781 gcaaccgggg atgattgaaa taaaaggcgc tgccgtcttc tccgtaggaa aagaagagaa
    21841 actgcccgga aacctcgtgc cagtaaagga attgcggcac ccacaccagg atgcacagca
    21901 gcgccatcac gataatcttt ggccattggc gcagtaaaac cggtacctgt ttcccgaaat
    21961 ctttcatggc tgcgatcccg aaaagcggga tcagaagcag cgcaacagca ttggtaggcc
    22021 ttatcagtgt gattaaaccg gccaacagtc ccagcaaaat tgaatatttc cagttgtaac
    22081 tattctcccg ccattgatcg accagataaa tgaatatcga aatcaatgca aaactgtagg
    22141 catgcgacat cggcgcctcg taagtgacat aatagaaaag gtttgttcca aagcctatga
    22201 tcagtatcgt aaatgctgtt gctgtgtcag aaaaatacct tagcagcagt tttcgcagaa
    22261 atatcagccc caggatgaaa taaacccagg cgctgaaagt aagcgccaca tgatagggaa
    22321 acgaataacc gtcggcagcg tatttacctg atatttttgc gtattcatgg gcgataagga
    22381 aaaaaggcga atacatgcac gacatgccca tggtggtaag aatcgccttt ttgccggtcg
    22441 gcgtctcaat gggccagatc catttacccc atgtttggat atccttttcg taaaatttta
    22501 acgtaaggtc cttttggata aaagcggccg gcaaataggc atagtaggat ttaatatccc
    22561 agaccacaac tccttctttc cctttatatt gctgatggtt gaaatcgaca atcggaataa
    22621 caatgatgta aataagtaca accagaaacg atagacttac ttttttaaat tttggcatgt
    22681 cttgaacgat gtttgataaa atgattcatt tcagctcttt aaatttcaat ttccaaggtt
    22741 gacatccggt atccggtgac gatcgtcatg aacttttcga tggtcaaatc taaacaaatt
    22801 cgttgatttt ctgtatttgt tgttgccagt atcattaccg cgggattgag aaaccgggaa
    22861 ccaaaggctg ccttgctaaa aattcaggcg ataagaaagg atgtgatcca aatatgcttg
    22921 gcatgccgat aatagtatat actgggtgtg aggctggtaa aaagaccggt gcgaaattat
    22981 ttcatttcct tctgtccggt tattgatggt tttgcattac ctgactatca acgcgaaaaa
    23041 tgataaatct tataaccatg gaaattttcg ttgcattcta ctttccggaa ggctcctgcc
    23101 aattttctat agatcatgtt ttcgggatca acctgtaccg accatccatc gagataaatt
    23161 gcgctctcgc agtttaacag aagggtatca ttcacctgat gtgcactgtt gattgggaaa
    23221 acgtggtcgg tattcaacct ggtacgggtg tttttataat ctttaaaata ttcagggtta
    23281 taatagtaaa cgaagccttt atcaatccat tccggacaga gatatacagc cgttttccct
    23341 gttttccact gccttattgt gtttaccagc tcctgcgtat tgcgctcatc gcctgtccgt
    23401 gggttgtaag tgaaaagcat caggaaaaca gccattaccg aaagcggata aaagatccag
    23461 ttcttgccgg agccgataaa atggatagaa acagcaataa ccaggtaata tccgatagat
    23521 ataaaaatca ggtatctgtc cagaaacatt ggcgttacaa gcgaaatcag gaatatcagc
    23581 aggtaaggta ccaggaacca gataaggatg acttttatat taccgggcgg aattcctgat
    23641 attttttttc tgttgaggat caacagtatt aatgccgtga ccattaacaa gatgaatacc
    23701 actgtgttaa ccggtgcatt gctgaatttc cataaattgg tgtataaatc ttcaggagtc
    23761 ggcgaaggca cccaggtccc tttatcattg aatgttgaaa aacggctgat cagtaaccgc
    23821 aggtatggca ggtaaagtac aaaggtcagc aggcagatga aacaatactt ttttaaaatg
    23881 gctttgcgga aatccctgat gaagacgacc gctatcacct gagccagtat aacaaaaaaa
    23941 ccaagaaagt gagagtatat caataacgta ttggaaaaca ataaactgat aaaatgcttc
    24001 cggccgccct tattcccggc taatgataga aatgaataca ttgaaatggt ggtcagtaat
    24061 gtaaagagtg gatacaccct tgtttcgtgc gcaaagaagg tgtgaaagtt ggaaaaggta
    24121 aacaacaatg aagcaagcaa agctacacgg acgttcaaga atttttgtcc aagtttatag
    24181 ataaaaacta cggtcaatga actaaatatt aacggcagaa ttctcaccga cactaccgag
    24241 atgccaaaca atttgatcca aaaatgcagt aatatgaaat acagaggagg attgttttca
    24301 tcctgcatca tggcggcaaa agtgctaaaa tcagcctgag agtaaaaaac ggtgaaaggc
    24361 tcgtcgcctc ccaacgatcc attgccgagg aatagagctt taattattaa gttgatggcc
    24421 agcagtaata tcgggattaa taaatcatag taaaattgct ttgaatgcat ggatttcata
    24481 agattctgat tttgggatgt cttcaaccag atatttattt aagctataat gctgataccg
    24541 ggatttgatt tctcaatgag ttgtgcctta tttcctgcct ccggaaaggt acaccttttt
    24601 tcagaatcag gaaagggctt atgagtaata ataccagatt ggatcaggcg cccgggatta
    24661 tttatcatta acctgacatt gaaaaccaat gacctgaaat gattgttatg gtttactaat
    24721 caagggggat tagatttccg gatccttttt ttgctccgta aatctgctcg gggtgcccct
    24781 tatagtatat attcccacgg ttcataattt tatattcaag gtagtaattc acattgatat
    24841 agcaatcatt ggtgctgaga gtagacatat acgtatatac ggttttcaga tcctgacagt
    24901 gaaagggccc ataaccgtta gcacatatga aatttacatt tgatattccc gagatattca
    24961 tgtcggccga accggtatga atagacatat gggacagttt ggtttttacg atcaggttga
    25021 tgtagccgct tccgccacgt gcatcgataa aaagcgaatc ttcccgaatg gtatcggcac
    25081 aggtaaccgt gccggtgctg agataatcaa gtttcagaag atggggcaac gttacattgg
    25141 cgatgagttt tctgtcgtag ctgcgcaccc agttgtatgt gttctcgttg cggatggcga
    25201 gcgtgctgtc gctgatttcg gttactatgc cggggagaat gttttcaccg ccctgcaatt
    25261 cgattttata ttcgtcgccc tggatgatat tcaggtccac atcgtcttct atgcggatgg
    25321 cggtgaaatt gccggtcacc tctcttgcaa cagtgacttc ctctccggtg ctcttcagaa
    25381 aatcgaatat atggtccttt ttgcacgacg taaggctgaa gagtacaaag gccaggacaa
    25441 atgcggcagg cagcttcgca ctctttaaaa accggtgacg ggaccttctc atgttcatat
    25501 ttcgattgcc ggatggctgt ggtattaaag tttttgacgg gtaacgggac taatgcggtt
    25561 tatatacgta caagggtaac cggtatccca atcccaaccc gataaaatca gcccttgcca
    25621 ggtgcgaacg gaaggtgagg gttgcgaaaa cgttcttgta ggcaagatac tgaagcccga
    25681 acttgaagta cccgaaactt ggcgccatgg tgccgaaaac atacatcgcc gtgttgaaca
    25741 tataggatag tttcgacatc cttaactggt aagccaggct gattcccatc cggtaaagtt
    25801 catagtcgtg tttaatcatg acatcctgaa gttcggcgaa ataagggtcg gattggtttc
    25861 gtgtgaaatc aaatcccagc ccgccgctgc ttttaaacga taacggtgca agcaggtcgc
    25921 ctgagaaatt gtaaaccatg aacgtctttc cgtattcctg tcccatgtct ttatatccca
    25981 tggtagcggc caggttcagg ttgacggatc ttttcccgtc gaattcaaat ttgtacaact
    26041 ccggcagaag tttgcggttc tggtaagcgt tttcgttgct caaccggtag gccagcgcta
    26101 cattgatcga aggggagtta atgccgtagt tcggggtttt ggtggatccg ttcgaaaaat
    26161 gcatcagtgc taagcccgat gccagttgaa cccgcgggct catcctccag cgatattcaa
    26221 ccatcaggtt tatggcggca ttcacatgcg aaccgatggc caggtattta taattggtga
    26281 cggggtcgaa acatttggtc aggtatccta atcccagtcc caccctgaaa ttcagctcgt
    26341 gtttcacgtc ccggacgatc gggtaattga tgtatgggaa cagggcattg gaacgcccca
    26401 ggaattcgga attgccgagt tgcgaatacc aataggatat cccgatgatc gggtatccgt
    26461 attccgattc ccatcgctgc tgcccgaaag tttctttgcc caggttgatc tcaaaggccg
    26521 ggaaatggct gttaaagatt ttcatctcca gatgatggtt gatcaaaaag ccatagttta
    26581 ccctgccttc gagcaaaagg tttgaattaa tcaacttatg ctgggctttg gaaaccgaag
    26641 gggtcgccaa taaagttaaa gcaatgaata taaatgcttt atgtgatggc ggatggttcc
    26701 ttactttact ctttttcacg ctggtcataa aaaaacggcc tggccggcca tttataaacg
    26761 caaaagtacg atcattcttt tattccgttg ataattattc atctgatacg tcctgaaacg
    26821 cacaaatccc catccccgta agttatctgg aacagctccg gggcgccctt ttcttttacc
    26881 actaaggcac tcagaacacg cagggcacta ggaaagattt accacggagc cacggagcca
    26941 aaggaataca gagaattgcg tattttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27061 nnnnnngaaa gatttaccac ggagccacgg agccaaagga atacagagaa ttgcgtattt
    27121 tcttagtgaa tctcagtggt ctcagtgcct tagtggttcc cttctctttt accactgaga
    27181 atgttctgcc ttgatttgta ttattcatct gatacatcct gaaacgatca aatccccatc
    27241 ccggaaatta tttgaacaac tgcgaatcat tcattcccgt acctttgcgg aaaaattttt
    27301 atatggatag ccgcctctct gaatttaccc gtttgctcga tataatggat gaactcaggg
    27361 caaaatgccc ctgggataag gaacagactt tcgacagcct tcggcacctg accatcgaag
    27421 aaacctatga actttcggat gcaatcctgg aaaaagatct taccgaaatc cgcaaggaac
    27481 tgggtgacct gatgctgcac attgtatttt attccaagct gggtgaggaa accggttcgt
    27541 tcggcatcgc cgatgtcctg aaaggcatca atgaaaagct tatccgccgg cacccgcata
    27601 ttttcggcga tgtgaaagtg acttctgccg aagatgtgaa agacaactgg gagcggataa
    27661 aactgaacga agggcgcacg tcggtgctcg aaggcgttcc catgtcgctg ccggccatgg
    27721 taaaagcata caggatacag gataaggccc gcggcgtggg tttcgactgg gagaaccgcg
    27781 accaggtatg ggagaaggta gaagaagaaa tcggggaact caaccacgag ctggccaacg
    27841 gcaatacccc cgaaaagcgg gaagccgaat ttggcgacct gctgttcgcc ctcatcaact
    27901 attcgcgttt tattgacgta aacccggaaa ccgccctcga acggaccaac cgcaaattca
    27961 tcaggcggtt tcagttcatt gaacaggagg cggcaaaaca aggcaggaag ctccatgaaa
    28021 tgacgctatc cgaaatggat gagatttgga accaggcgaa aaatttagaa taaaattatt
    28081 atttatcaga ttgttatgag aagagcgttt ttcccgatat tgctgttttt gatattctcc
    28141 ggatcgtcat ttggtcagag tatgagaatt catggtattg ttacagattc cgaaggcgtt
    28201 ccgattcctg atgcctggat acaagacaat ggccgtgtcg gatatctgac gattaccgat
    28261 tcatccggtt attattcagt aataatcaat tttactgaaa catccggaga gatgcttttt
    28321 gtaacgtgct cttcatttgg atatcaggac ataaaagtgc taattgatac ggcgtttcag
    28381 aatttggttc tttatccaaa gatgggaata cacactaata gtttacacag aaattatccg
    28441 gcaagtttag ccggaagata ctatggatat gacccaaaca ggataaatgg atcaatagat
    28501 tattattttg aattcaatca tgctgatttt tcaggttaca gagatctgct tgattcaacc
    28561 aatattacct ccctcggtaa gctatccgca acgaaatttg atgtttcatt gatgcttcaa
    28621 aactatatgt taaggattgg ctatggctca ggatcggatt taaccattga tgaagactcg
    28681 gtgagccgcc gttacaaatt atcattggca ggtattgaac tggggttttc aattccgata
    28741 ggatctcata tcatgatcaa ttacctggga ggtatttgca attaccggta tcggatggtc
    28801 aataaatttt caaatggaga cgtatctctc acaaattatc ttaaaaaacc tgaaattgac
    28861 ctgcgattta atcagccatt tgtatctaac cgtctggagt tgggctatcg tacaaactgt
    28921 ccggttacag aatcacctgt tgatttttat atcgctttct ttaccggttt taattcttcg
    28981 atttcgcgtt ctacattagt ttgttccagg aattcacgat tattatctga ccagtcaatc
    29041 ggatttgatc gttttattta tggcctgaag atggctgtga ttataaaaaa tcgtattcac
    29101 tagattttca acaaaagtct ggcagattgg ccttgtcgat tttctgggtt ttcttaacca
    29161 gaatgaaata atcttttcca tacctgttcg ttattataat aaacatcatg cttaagatat
    29221 tgccttttct tacgttaatt ctgattcttt ttgggtgtca acccagtgtt acattcaccg
    29281 agccccagcc taaaggggct aaacaccttg accggattcc gtcaaagctt tcaggccact
    29341 acctttcgga ggacaaagcc tcgtttatcg ttatttcgga ccgcctgatc gttcagactt
    29401 atgattatga ccttaagacg gctaaaaccg acttgctccc gcatcagaag ctggtgggcg
    29461 attcactcga aaatacggaa acaggcagcc gggattttgt catggttacc ggcgacagcc
    29521 tggtgcagca tattcacgaa accgatacgc tgtttttgtt atcggacggt caggtgctta
    29581 caaaattcaa agggtattat ttcctgaaca cgaaatggga tcagaattgg tacgtccggc
    29641 aactttcttt gtcgaaaggt gtcctgaaac tggcagagat ttcaacgagc gaagaaattg
    29701 ataacttgcg gcagattacc gggacggaag aagattccac catttacaat tttagtcccg
    29761 gtaaggaaga attcaggaac tttatcaacc aggatggatt tcacaaaaag tcaagatttt
    29821 acaggatatc gcgctgaccg ttgccctcag gatcatctgc cgggcggttc cgggctaagg
    29881 ggagcgcatt cgccccgtga atggctgaca atcctgagaa aggttggcgg ggcgccggtg
    29941 atcctgctct ttgtttcgtt cttcttaagc tccggtattt cagaagccca gacggccgat
    30001 tcgtctactt ccggttatac cgtcatcaac gggccctacc tgaaatcgta tgttaccgat
    30061 acccggagct tcgtaaaatc gccgttgcag tggaaaacaa agcaatggct gaccatgggc
    30121 gccgttgccg gcgccggcgt cctgctctat tcaaaagatc cggaaataag ggattttgtc
    30181 cgggagcatc ataccgctgc cggcgacgac tttttcaggt acggtttcga gccctggggc
    30241 agcgggttat atccggttcc cctgttaggc tgtatgtacc tcacggggag gctggcgcat
    30301 aatgaccgta tatcggcaac ggcgcttacg gcaggaaagg cagccctgat cagcgcctta
    30361 tttgtgaata ttaccaagca gcttatgcac cgccaccggc cttaccagga taatccggcg
    30421 aattatgcgg gctggcatgg tccgttcagc gactggcgct atgccagctt cccttcgggc
    30481 cattcggcct ttgcattttc aatagccgcc gtgctggcaa gcgaataccg tgaaaccggc
    30541 tgggtgcccg ttgcagcata tgccgtggca gccggaacgg cattttcaag gatctatcgc
    30601 gacaagcact gggccagtga tgtctttgcc ggatcggcgc tgggcatcgt gaccggaaga
    30661 ttcctctgga aaaagaaccg gacagtaagg attacccctg ttctgtcagc gcattattcc
    30721 atgatttcag tcgaagtgcc ccttggatgc cgccattccc ggggcgacgc acggtgcgaa
    30781 tgagccccgg ccgcgaataa aaacacaatt tcatcttatt ctgatggtat tctatcctgt
    30841 ggaatttagt atattcgtac gtttttatat ccaccagcca tcaaacaaac attctgcctt
    30901 atgaaacata aaatactcct ctttttattt gccggtttac tggtttcctt taatactcag
    30961 gcacaggatt tctggaagtt aattcctttt cctgattctc tcggtatctc cgctgttact
    31021 ttcaactccc agggagatat ctacgtaaca acgggtactg atgatgactc tattgatgat
    31081 ggggtacttc gctcatccga ccagggacaa acctgggaag tcttatataa caatagctat
    31141 tttgcagtcg ggcctattgc tgttaatgat tccgggcata tctacatagg tgtcacacac
    31201 aggcaatatg gattgcgggc ttcttatgat aacggcaact catggtctat aatacaacac
    31261 cctgcattag gcgaattgac gaaaataatt tgcgtgagta cagataccat attagtgggt
    31321 gcagccaaga gtggaggggt actgttaatg ctaacccctg accgttgtat tacatgggat
    31381 accctgtttt cgaccagcaa tcataccagc gaaacaattt cggatattgc cattgcacct
    31441 gacgggacta tatatatagg attgatgtgc tttatggaag atatgggagg ggtatataaa
    31501 tcaacggata atggaattac atgggaatat gtcgggtttc acaataacca ggtatcgaat
    31561 atagagctaa atgccgaagg cgatctgtat gttggcgtat tcggcagttt tactgattta
    31621 aatggcgggt tatatgtaat aagaaatggt tcagctacaa ttgatacctg tttgtacggc
    31681 ccttcaatta acgggcttgt ggttaacact gcaggacata tatatgcaag tatagcctgg
    31741 acaactggaa tattcatttc aaaagatgat ggggaaacat ttgagataga aagtagcgga
    31801 ttaccaatcg gtccaatggg taggctgtac gttgaccagg atgaatatgt atatgctaaa
    31861 actgacttct cttcatcttt actgtacaga actgttgaac ctacaataac tgatgttaag
    31921 aataaaactg agctatcaag tccaaactta ttagtattat acccgaatcc ggcaaccgac
    31981 cggcttaccg gcaggttaac caggaaaggc aactgcgacg ggagatacag gctttcg
//
LOCUS       DAOPYV010000032        31560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_1362_length_31560_cov_12.814061, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000032 DAOPYV010000000
VERSION     DAOPYV010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 31560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31560
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1362_length_31560_cov_12.814061"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            435..1835
                     /locus_tag="PKI35_05915"
     CDS             435..1835
                     /locus_tag="PKI35_05915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX43956.1"
                     /translation="MKLKFLGAAREVTGSKHLITTENGKKILLDCGMFQGKGLETDAM
                     NRNLGFDPAEIDHIILSHAHIDHSGLIPYVYKLGFRGSVVCTNATRDLCAIMLADSGH
                     IQEGDVKWFNKRRVAKGLTPIEPIYTEKEARECMELFIGVAYNRKFRIDGHTLVKFTN
                     TGHMLGSSVVSLEITEGEKVTRIGYTGDIGRPVNRILKHPQPFPQCDYLIAESTYGDR
                     LHPAMQDAEAELLRVIRETCVEKGGKLIIPSFAIGRTQEVVYTLNNFFNEGLLPKINI
                     YVDSPLAVNATEIFRMNTDCLNEEVHEVMKTDADPFGFGSLFYLKSPEESKKLNSYKK
                     PCVIISASGMMEAGRIKHHLANNIENPNNTILAVGYCAPVTLGARILNGENPVSIFGE
                     KHPVNATIERIEAFSGHGDYNEMRNYLKCQDAGQVKKVFLVHGDYEAQQFYQKKLEET
                     GFKNIMIPSSGTEVKL"
     gene            1935..2456
                     /locus_tag="PKI35_05920"
     CDS             1935..2456
                     /locus_tag="PKI35_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43957.1"
                     /translation="MEKISDFCGRELQWMQPRMTEPYFELKAADEKVASLKFKNAFGS
                     FAEGTFAGGIWTFKRQGFLSSVVTIREKGYENNLAVYRNNTWSEGGTLEIPDGVKYKV
                     NSNYWNEQFEYTGESGNLLIQCTKISGFKLHARLEIFPDACELDKYPWMLLLGWYLAV
                     MSSREGEFMAGLF"
     gene            2620..4089
                     /gene="cysS"
                     /locus_tag="PKI35_05925"
     CDS             2620..4089
                     /gene="cysS"
                     /locus_tag="PKI35_05925"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNX43958.1"
                     /translation="MSEKLYVYNTLSKQKELFVPINPNHAGMYVCGPTVYGDPHLGHA
                     RPAITFDLVFRYLQHLGYKVRYVRNITDVGHLENDADEGEDKIAKKARLEELEPMEVV
                     QYYSDRYHEYMDRLNVRKPSIEPRASGHIIEQISMIRKLVDSGYAYESNGSVYFDVEK
                     YNQTEKYGILSGRKLEDLLTNTRELEGQDEKRNPFDFALWKKAQPEHIMRWPSEWSDG
                     FPGWHLECSVMSTKYLGETFDIHGGGMDLMFPHHEAEIAQSTACNHKQPVKYWMHNNM
                     ITINGQKMGKSLGNFITLEEFFTGSHAALEKAYSPMTIRFFILQAHYRSQLDFSNEAL
                     QAAEKGFDRLMKSMETLRRITPQNTSAVDIPGLEKQCYDAMNDDFNSPILIAGLFEGV
                     RIINSAYDGKENLTADDIKGLEKIFNTFVIEILGLQPQVDVNAGSNVVDGLMELILEI
                     RKNARANKDWATSDKIRDDLARINITVKDTRDGATWSLE"
     gene            4133..5815
                     /locus_tag="PKI35_05930"
     CDS             4133..5815
                     /locus_tag="PKI35_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HNX43959.1"
                     /translation="MKPVKTLSALLVITLLISSCANKTEKMEKELRSFIARYDSVVQP
                     LQKAAYLASWDASISGKPEDFKKSEDLSIELTKVYANKDDFAVLKKIKESGAVKDEML
                     SRQLTVLYNAYLGAQVDTAKLNAVIKLQSQIEQKYGNFRANVNGKQLTDNEVENILKT
                     SVNNKELQQAWEGHKAIGPVVADDVKKLVKMRNEIAKELGFNNYHEMSLKLSEQDPEE
                     ISKLFDELDNLTRESFAGLKDEIDTYLAARLKIKKEELMPWDYQNRFFQEAPAIYSVD
                     LDKYYKGQNIEKLTTDYYASLGLDITGIVANSDLYEKPGKNQHAFCTDIDNKGDVRVL
                     CNIVPNYNWMNTMLHEFGHAVYDKYIDQQLPFTLRNPAHTFTTEAVAMFFGRMASNAN
                     FLVDMKLTDTADARKVAEESFKVLRLEQLTFSRWAQVMYRFEKSMYENPDQDLNKLWW
                     DLVEKYQMLKRPAGRNQPDWASKIHIALYPCYYHNYQLGELLASQFNHYLAANVAKSD
                     NMRMSYFGQKEVGKFFIEKVFAPGAQYEWNDMIEKATGEKLTPKYYAEQFVK"
     gene            complement(5893..6609)
                     /locus_tag="PKI35_05935"
     CDS             complement(5893..6609)
                     /locus_tag="PKI35_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX43960.1"
                     /translation="MKIQTIAIDDEPLALKLISGYIRQTPFLECAGEFDNPISAIEFI
                     ESHETDLVFLDIQMPDLIGTDLARYLQNGPKIVFATAYEKFALEGFRLQAVDYLLKPF
                     GYDEFLIAANKARNLIELEQKTYPLMETNDEFLFVKSEYKIRRINYDDVLYIEGMKDY
                     VKIFLRTDARPILSINSLKTLEEKLPASQFMRVHRSFIVNLNRVDTIERFRIVFGKTY
                     IPVGEAYKEHFQKFLNENFL"
     gene            complement(6613..7752)
                     /locus_tag="PKI35_05940"
     CDS             complement(6613..7752)
                     /locus_tag="PKI35_05940"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNX43961.1"
                     /translation="MADLKDIKHIKMPLPGKKLLPYLLHGFVWSVIFILTTYFYLSGN
                     KTDTDVLLHNYRNLLLYAAIFYINYLVIIPKLFFQNHKIWYLAAALSLIVLTTFLVSS
                     TFRRQPPMRFPAPGFRQEQPMIQQPRLRNQFPAPPPGQKPTPPMNLPVYNFFITALLV
                     TGISLGLSFSGLYIEQEKQRKEAEKEQLNTELAFLKNQINPHFFFNTLNNIYSLVQTN
                     VDDGQKAILRLSKLMRYLLYETDKESVPISREIDFMKVYLELMKLRLSPKVDLRVKFP
                     EDFPDLSISPLLFLPFIENAFKHGISNRAFSFIHIEMGVKDDCINFKCSNSLFPGNVQ
                     AEGASGIGLENVKKRLRLLYPGQHELKIVGDGSAFIVELQINPVS"
     gene            complement(7759..8436)
                     /locus_tag="PKI35_05945"
     CDS             complement(7759..8436)
                     /locus_tag="PKI35_05945"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNX43962.1"
                     /translation="MNNRFALLLILFLPFIPGYLSAQTNDAQLWMGVNLEKKLTPEFS
                     LYFAEELRMNENITEAGTIFSDLGLGYSITPGLKVSANYRFISKRRIDDSYSMRHRYY
                     FDLSYRHKFMPLILSVRARYQSQYTDILSSEDGKIPENYERTRLKLGFDTGKAFEPYL
                     TTEAFIPVGKQYDSPVIDCMRYTAGMEYKFYNRHSIDAWYMIQHEMNVPDPETDFVIG
                     LGYSFVF"
     gene            complement(8429..9142)
                     /locus_tag="PKI35_05950"
     CDS             complement(8429..9142)
                     /locus_tag="PKI35_05950"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNX43963.1"
                     /translation="MTHTKFAIILFFFLLFAAPAGMRAQVFETSFETQVDDGGSKKSD
                     KKKDKDIIQINWSFFINLGIDITAVLALIFLVYYPAYKNQDYIFTFLIFNLVIFVLTY
                     VLNEVKISMGAAFGLFAVFSMLRYRTAGISIKDMTYLFIVIALGLVGAIRLEYYELAV
                     LDALVIAFAALLECRWIFKRESSQYVRYENIELIVPEKRQQLISDLEKRTGLNIHRVT
                     ISKINFLRDTADIKVYYYE"
     gene            complement(9139..9885)
                     /locus_tag="PKI35_05955"
     CDS             complement(9139..9885)
                     /locus_tag="PKI35_05955"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNX43964.1"
                     /translation="MKLKEDILSSFQSISLEQMDSVKLLNRVDTKFIFNLDRLPEVLS
                     DLGDQYYILETDGQRLYPYLTTYFDTDQFSAYVNHHNGKLNRHKVRIRTYVTSNQHYF
                     EIKFKDNKKRTIKTRIRVPDNEPVISGEKEKLLLKHSPYTANELSPKLTVKFSRMTLV
                     SKKMNERITLDTNLIFIHDQLEKSYDGLMIAEVKQGKTEVSPFIRRLHHLHVNPARLS
                     KYCIGVISMYDGIKYNQFKETLLNINKLVS"
     gene            complement(9894..10457)
                     /locus_tag="PKI35_05960"
     CDS             complement(9894..10457)
                     /locus_tag="PKI35_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43965.1"
                     /translation="MKFPAIIVLTCLCMVQGYGQSKKELTAARAKSSTEWTAKKEDGK
                     TVSYKSLYEEYDKNGNTTLHIEYDEGGTILLKEITVFDKYKRISSETVYEAAKGKTIM
                     TTYLYDAFNNVTEEKEFSGDKLIKKTVCSYNSKGQKNSEITYDGSGDVLKKSTYTYDS
                     KNLKTGRSTVNTAKQTETTKKWVYTYF"
     gene            complement(10463..10876)
                     /locus_tag="PKI35_05965"
     CDS             complement(10463..10876)
                     /locus_tag="PKI35_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43966.1"
                     /translation="MITSAKNTIRNVAFVLLIGCIAASCEKDPGEGGTSSIRGKVWVK
                     NYNSSFTYLQEEYYAQDEDVYIIYGDDFSYSDRVKTSYNGAFEFKYLRKGDYHIYVYS
                     KDSTCTSSADIPVITDVTISKNNEVVELPDIVIKK"
     gene            complement(11426..11869)
                     /locus_tag="PKI35_05970"
     CDS             complement(11426..11869)
                     /locus_tag="PKI35_05970"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNX43967.1"
                     /translation="MKTNILLLILPVLFLSNACMNQQEKSAAIPETDSLMAPWMEAWH
                     AGDTGQIAACLTPDAVVILPDTMMTGINLLRKAWIGPVAKSLRNLAIVNKNEEKSGEI
                     ACISGSYTNDWVQNDSVVGHAKGYYTFLFKKQADNRWKISVVNLN"
     gene            complement(11911..13875)
                     /locus_tag="PKI35_05975"
     CDS             complement(11911..13875)
                     /locus_tag="PKI35_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNX43968.1"
                     /translation="MMKTMQSVITRGLFMILLLWFSGNVLAGGGPSFRELHSLKLPFK
                     PSYFMYAHTGDFRFFLCSSKTDMLMFDGATGKISWKINFKTDFANQKFEYQLWNKDAN
                     VILLYDEDTKKGIAEKYFLDGKTGKLLWKSDKYVSDYGKYELSEGFENYFDAETNGVL
                     LPARDNVDLVDVYTGKVIWSKNFELTGKAKQFDCYIMNYYNLVKIITGKEESIYLTTR
                     EGREVTETDPYFDQKKYMADRKHATMIGIPEKNMYIILQGETNRVFEILGALSGQPTD
                     VPKWKMNFIAYEEGTNRELWRKKYTIGYVFDWIDNTPMIRMNYADGKLFVTHDPALKT
                     NDGLTVIDVNTGEKLWTAYYSSSETKNGLSKTTLTPFPAPLPVVSEGKAYVVDKVKNT
                     VRCYDAQNGTLVWETKKYPDAQKIPALLVTNGVVMLAYGSPASKIVREEVRTGNSTRT
                     VYKRLFLDKDKYGLIAYDAKTGAEIWNGDQAGKKAKDNFSYLAGVEYIDGKLYCATDE
                     NFFILDPPTGNVIKSIPVSKEKLGKAWKLIYFPEKQKMILNCSKGIIKIDPLTATIEG
                     VLKTPNVSAYPASVHMNADNDYADYAIFTDGNSEKLDFKTFASIDLDKMKVRGTGDAA
                     LLFYDNPHFSDGAEMFYQSDGSEISIFSIE"
     gene            complement(14157..14789)
                     /locus_tag="PKI35_05980"
     CDS             complement(14157..14789)
                     /locus_tag="PKI35_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX43969.1"
                     /translation="MEREIKILIVEDDAAVREGLRMLIDGSVGYHCIAACPSAEEALK
                     ILPGEIPDVVLMDINLPGMNGIECVVCIKSVHPEVQVMMLTVFDNTEEIYKSLAAGAT
                     GYLLKKTPPAKLLEAITDLVNGGSPMTGEIARKVVQTFMKPALTQFPEANLTPREEEI
                     LSYLAKGYLYKEIANALFINIETVRTHIHKIYQKLQVRTRTEVLLKCINH"
     gene            complement(14860..17700)
                     /locus_tag="PKI35_05985"
     CDS             complement(14860..17700)
                     /locus_tag="PKI35_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNX43970.1"
                     /translation="MISQVLQDSRGLIWMVTGDGLQCFDGMRFRSFKVPYSGTAGGSE
                     SIMREIVENKPGEFLISTNSSILNFNTSNGIFKSVNRKPGYCPRILKISFKKNPLIWL
                     PDKKLCCIKGEQIIRLRLNYRDKMEPPADFFPEEAVETASGDLLVYNDKGFLIIENFR
                     KNRTGEFLSRWHGMRDGCQGLANGKDGKTYILSGKNIYTFLPSGRLELFSETVRVRDG
                     KMFIDRYGNFWISDKGKKSVYRMAGKHTSEIRLITRQGKHTDTIQSPVIGFFEDKQGN
                     LWLGTDGDGVLVYSPGKMQFNLAKTGFTRTIESRNGQLWAGTYRNGLWKLSEDFSKVS
                     RLAHTALPANLYYFDLAFDRQSRLWAVTDQGLFVIDTLGNLAYRHPFTSNAATFLFVT
                     PDTVALDAECQLYLYAPKDKPRLLDSKEFGNIRKFIQAAECYWLATPFGLYRSKASAG
                     FFRQMIFIKKNRISLKPVNDILYLDNCIWSATETGIDRFDIRGRRLPELKALAELQNE
                     VIYSLVPDALNRIWFTGNRGIGCIPQERNSIIWFNARNNLQSLEFNGQASHKTTDGRI
                     FFGGINGLNGFDPAGFRPQMRAPGVRLISLFVADTAYSGGIPPENPEIRLSWQSPHIS
                     GRVFTDDYENPEMQSFSFLLEGYQKEWGNMSVNPEFVYRNLPPGHYRLFVKCSNAYRN
                     QSKATCLLTLTITPPFWRTRWFLILSAVLTIFVTILIVKGFQDRRFKNRLHDLEKQNA
                     VNKERLRIAKDMHDEIGASLTRISILSELAKSRQAEADQSMKIIGQISEISGNVVDEM
                     SEIIWAMNPKNDTLDSFSSHLRQYASGYLETAGIDGLFNFPAEIPPAPMTSESRRNSF
                     LTVKEALHNIVKHAGATTVRLNLSYAENTLSIGITDNGKGFDPGNRLGTGNGLVNMQK
                     RILEIGGAYTLVSEPDKGTEIRFTVKLDPASE"
     gene            complement(18098..19375)
                     /gene="tyrS"
                     /locus_tag="PKI35_05990"
     CDS             complement(18098..19375)
                     /gene="tyrS"
                     /locus_tag="PKI35_05990"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNX43971.1"
                     /translation="MNFVEELRWRGMIQDMMPGTGEMLSMGMATAYVGIDPTADSLHI
                     GHLVGVMMLKHFQIAGHRPLALIGGATGMIGDPSGKSEERNLLSEETLRHNQECIKKQ
                     LLHFLDFESGAPNAAELVNNYDWMKEFSFLGFLREVGKHLTVNYMMAKDSVQKRLETG
                     ISFTEFTYQLVQGFDFLHLYETKNCRLQMGGSDQWGNITTGTELIRRKTGGEAFALTC
                     PLITKADGGKFGKTEKGNIWLDPERTSPYAFYQFWLNCSDEDSKKYIRIFTLFSREEI
                     EAMEKEHDAAPHLRILQKALARDITVRVHGENEYNQAVEASEILFGKGTTETLKHLDE
                     RTLLNVFEGVPQFEISRADLEKGIGMADFLAEKTSVFPSKGEARRMLKDGGVQLNKEK
                     AAEDRIVNAGMLLNDRYILVQKGKKNYFLVKAV"
     gene            19598..20860
                     /locus_tag="PKI35_05995"
     CDS             19598..20860
                     /locus_tag="PKI35_05995"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNX43972.1"
                     /translation="MEKENTISCPNCGAEINVSEILYHQVQDQLKKEFEAKIAEKEKD
                     IQKKSAYLQSEYEKLVKDKENLQAQVDKEVKVRLGSEKSKLEETIRLEVRNETSGQIQ
                     TLQQELEQKSKQVSEFNKALAEIEKLKREKEELRDAVVLEKEKEFSEKLKDEKLKIKR
                     QTDEEVALKIKELEKQLEDQKLLAEEMRRKADQGSMQLQGEVQELAIEEILTSLFPFD
                     LISEVGKGVKGADVIHKVRNKLGVDCGTILYESKRTKAFSAEWVTKLKADALGTKADI
                     CIIVTETLPDGIDRIGQKDGVWICSFNDFKGLVLVLRESLVKINEAYASQTNKGEKMQ
                     MLYDYLTSNEFKMQLGAIIEGFSDLQNSYIQEKRAMERIWKQREKQLEKVLLNTNHFI
                     GSIQGIAGSSIPGLKQIGPSDNLFELEE"
     gene            complement(20882..23617)
                     /locus_tag="PKI35_06000"
     CDS             complement(20882..23617)
                     /locus_tag="PKI35_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX43973.1"
                     /translation="MRSDIFSRKTMVIAIAAFLTAGFFLTAVLVNRSPERARKKYERY
                     LRETAAISIGKSGKEPGKEKEADSPDMAALQEFFMTADPAEKRVPAERLIEANRILQK
                     LSRTRAGGYQLQWSGTSSEMGGRTRAIMWDPNAENKAWAGGVTGGLWYNNNVTSPLSQ
                     WSPVNDFWSSLSISCITYDPANTQVFYAGTGEVQTALITYRESSGRGVGIWKSADGGA
                     TWELLPSTSNFAYITKIAVRNENGTGVIYAGVSSGYYHGANQQSLPSDGLYRSADGGS
                     TWQQVLPLIPGSDVPYAVSDIAVTPSGRIFVGTMQNLNDAGGACILSSDNGTVGSWIV
                     YDDISNLIIAQPQYKKPGRVMLAPAPSNGDIVYAAIGAGYLNANGHPVYYGRYIIKTT
                     DGGATWDQLNKPTTSGDWANLAWHALTITVDPNNPETIYTGGLDVWRSTNSGTNWQHL
                     SDWAMMYYGGGDTYVHADQHIMVYKPGSSSTALFGSDGGIFFTSNANGSPPVFQQKNS
                     NYNTLQFYTCAIHPTAGTDQYIGGLQDNGTLLYNGSPLSISNMIDGGDGAFCFWDQDQ
                     PNLYLTSYYYNRYTVFQNGTWVSNIDNQSGVFVNPADYQNNNNLLIANAVDFVGTHAN
                     QVLRVPDIPQNGNGVFVDVGTFCEGYFSHVKISPFSNESSTTLFLGTVSGRLFKVNNA
                     QAVPQTTEIGSPEFPASNISCIAIGGSEDTLLVTFSNYGVSSIWQTYDGGEQWNEVEG
                     NLPDMPVRWAIYHPQNSRQALIATELGVWSTSNLHDAQVYWQPQTTGMANVRVDMLTL
                     RTSDNTVLAASHGRGLFRAEFPLDLNTAINSAESQDIKIYTDGSGGKLTIETKNSLGK
                     HGEISLFTLGGKKVMDQDFTVSALHSFSTGPLVPGVYLVKVVTDNRIYCRKLFIR"
     gene            24060..24743
                     /locus_tag="PKI35_06005"
     CDS             24060..24743
                     /locus_tag="PKI35_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX43974.1"
                     /translation="MKKVLISLTAIAIAVFSSVNAYSQSSFSFHVGPAFPISDFGNDD
                     VDDDDAGGANVGLNVGGKYIYPLNNNGLGLFIGADLNYNGLKGSVKDYIEDEYRYLGV
                     DDEDISYYKFINVPVTAGLSGTYSGNGQVSVFGEVGIGVDFLKITDLTIEVNNKDMVT
                     NFDMSAQLAYVIGGGVWINDKVGIGLHYNNLGKHEINGEMKYSGYTEDLESSDLKVGL
                     VTLTLGVKL"
     gene            complement(25038..25559)
                     /locus_tag="PKI35_06010"
     CDS             complement(25038..25559)
                     /locus_tag="PKI35_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43975.1"
                     /translation="MKRLFLMIPLLAAFGFICSCSKDSDEPARENQWKANFDGKDSVY
                     TITKATLQTVSGRQHLSIITELNKTCFDMYVNAPNITTKEYPIASPFSYPFPDEYACC
                     YYRILQPNSYYTARAFVSTVASPEYKVVITELSDTKVKGTIKFKGVDQGYNTSGEMVI
                     SASFSTDLLETGN"
     gene            complement(25888..>26889)
                     /locus_tag="PKI35_06015"
     CDS             complement(25888..>26889)
                     /locus_tag="PKI35_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HNX43976.1"
                     /translation="QFVGNKSGDEGVRFSGNELELTGELKDCLQNYFLTSFRDCEYYN
                     LYHESDICLNEVYTYVSAIFEDPGCLHEQSVNLAKHLYEQSVHPKIKGGEFYVVYFSD
                     CIIEGETVDAVGLFKSENKDTFLKVYQEQDGFTIDAETGININRLDKGCLIFNSEKEK
                     GYLVSVVDNTGKGNDAQYWTDRFLHVRPRNDGFFQTKNLLSLTKSFVVEKLPEQFEMT
                     RADQAELLNRSVKFFREKDSFEMDEFASEVLAEPEVIDSFKSYKSKFEQERDIELNDN
                     FSISEAAVKKQSRVFRSVIKLDRNFHIYVHGDHQKIIKGYDEETGMHFYQLFFKEES"
     assembly_gap    26890..26989
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26990..27676)
                     /gene="truB"
                     /locus_tag="PKI35_06020"
     CDS             complement(<26990..27676)
                     /gene="truB"
                     /locus_tag="PKI35_06020"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNX43977.1"
                     /translation="MAFDFETGEIILVNKPYRWTSFDVIGALRSFFKKELAMPKVKIG
                     HAGTLDPLATGLMILCTGKATKRIEEFMGLEKEYTGTFVLGASTASFDLEKPVDQVFP
                     TEHITDGMIADVVSSLTGDIMQVPPIFSAIKINGKRAYKSARKGADLELQPRSVNVRE
                     FEITRLAMPEVDFRIVCSKGTYIRALARDFGQALGSGAYLSALCRTRIGPYRLADAYE
                     IEQFKELLRKE"
     gene            complement(27676..28482)
                     /locus_tag="PKI35_06025"
     CDS             complement(27676..28482)
                     /locus_tag="PKI35_06025"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNX43978.1"
                     /translation="MSWLQAIIIAIVEGITEFLPISSTGHMALTASFMGIEKEAFTKL
                     FIENIQFGTIISVIVLYWKKFIDFKKFSFYLKLIVAFIPAAIVGILLKEHIDRALGSP
                     LFIAVVMFLGGIILLFVDNWFKNPIIRSEEQMTYRNSFIIGLFQILSVIFPGLSRSAA
                     TIIGGMQQKLTRQAAAEFSFFLAVPTLAGAFAKSLWDTFQDAPQILTSYNIRLLALGN
                     IVGFLVAMAAIKVFIKFLTDHGFKLFGYYRIIVGVIIIGLLLAGINLSVV"
     gene            complement(28484..28765)
                     /locus_tag="PKI35_06030"
     CDS             complement(28484..28765)
                     /locus_tag="PKI35_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HNX43979.1"
                     /translation="MAKETKVVPATPAKRKFDEKPPQFALTRENYIIMGIGVLLIMLG
                     FILMIGGGSKDPNVFNYDMFNFKRLTLSPILILSGFVVELFAIMKKPKE"
     gene            complement(28806..29693)
                     /locus_tag="PKI35_06035"
     CDS             complement(28806..29693)
                     /locus_tag="PKI35_06035"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNX43980.1"
                     /translation="MTNSQSNESFGQRRLRTSYVTSIISIMLVLFMLGLLGLIILHGK
                     KLSEYVRENIRISLLMKEDSSESMILTYKQKLDRTRYVRSSQYISREEAARELTEDLG
                     EDFIHFLDFNPLPAAIDLQLKSEYTNNDSISKIEKQLKSNPLVKEVVYQKSMIDVVND
                     NIRKISMVITGFSMLLIIIAIVLINNTIKLSIYSRRFLIRSMQLVGATESFIRRPFIR
                     RSMLHGLIASLLAIGFLMGTLWIARQRIPELIQLEDMRSFTVMYGLLVMIGVFLSGLS
                     TYFAVRKFLHMKADSLHMS"
     gene            29872..>31560
                     /locus_tag="PKI35_06040"
     CDS             29872..>31560
                     /locus_tag="PKI35_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNX43981.1"
                     /translation="MDYNFREAEKKWQKYWADNKIYKSEIDHSKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIFARYKRLKGYNVLHPMGYDAYGLPAEQYAIQTGTHPAVTTEK
                     NINRYREQLDKIGFSYDWSREVRTSDPKYYKWTQWTFIKLFHSWFDRQEDKARPIETL
                     TGIFETEGNRNVKAACSDGTPLFSAQEWASYDEKQQQEILMKYRLAYQADTVVNWCPA
                     LGTVLANDEVSEGHSVRGGHPVERKKMRQWLLRITAYADRLLKGLDTVDWSESLKEIQ
                     RNWIGRSEGGNMNFAVENSDAVIEVFTTRPDTIFGATFMVLAPEHELVSVITTPEQKD
                     KIDAYVTWAKNRSERERMTEVKKVSGEFTGAYALNPFTGTKIPVWIADYVLTGYGTGA
                     IMAVPAHDSRDFAFARHFNLPIIQVVNKPGHEPSDPSTWEESYDSHDGIMINSGFISG
                     MEVNQAITEVIRKAEEMKIGTGTVNFRLRDAIFSRQRYWGEPFPVYYRDGMPYTLPES
                     QLPLLLPEVDAYLPTETGEPPLARAKNWVTADGCRLETNTMPGFAGSSGYYFRYM"
BASE COUNT         8251 a         7295 c         7552 g         8362 t
ORIGIN      
        1 agcttgccac tgaacgctat caggagatat tggataatct gggatagcaa atgcctgtga
       61 atattatccg gatgaaaata caaaaaccac gaaatgctaa taaacaggaa tttgaacagc
      121 gtacaagccg gcttcgggga ctctttggtt attagcttac gctgagctcg tccttcgccc
      181 tcggttcccg attcaactaa atctcctgcc aaactatgca cgtaaagccg gtttcaatat
      241 tttcctgaaa atattacagg caaaacgtat tatttatttt ggccatcagt cacgcttaat
      301 attcttaccg gaaagccatg attccggagt ttattgtgta agtttgatgg ggcaaaaatt
      361 gcctgcttat ggccggcaag gccgtcccga tgaacaacag aatccaattt gtcaggtaaa
      421 aattcagcaa gaatatgaaa ctcaaattcc tcggcgctgc ccgtgaggtt accggcagca
      481 aacatctcat taccactgaa aacgggaaaa agatcctcct cgattgcgga atgtttcagg
      541 gcaaaggcct cgaaaccgat gccatgaacc ggaacctggg tttcgacccc gccgaaatcg
      601 accacatcat cctttcgcat gcccacatcg atcattcggg gctcatccct tatgtttata
      661 agcttggttt caggggatcg gtggtgtgca ccaacgccac ccgcgacctg tgcgccatca
      721 tgcttgccga cagcgggcat attcaggaag gtgatgtgaa gtggttcaat aaacgccggg
      781 tcgccaaagg ccttacgccc atcgaaccca tttataccga aaaagaagcc cgtgaatgta
      841 tggagttatt catcggggtg gcctataacc ggaaattccg gatcgacgga cataccctgg
      901 tcaagtttac caatacgggc catatgctgg gcagcagcgt ggtgtcgctc gaaataaccg
      961 aaggcgaaaa agttacccgc atcggttaca cgggagatat cggaaggccg gtgaacagga
     1021 ttctgaaaca cccccagcct ttcccgcaat gcgattacct gatcgccgaa tcaacttacg
     1081 gcgaccgtct ccaccccgca atgcaggatg ccgaagccga attgctgcgc gttatccgtg
     1141 aaacctgcgt tgaaaaaggc gggaaactga tcatcccctc gtttgccatt ggccgtaccc
     1201 aggaagtggt gtatacgctc aacaacttct ttaacgaagg gctgctgcca aaaatcaata
     1261 tctatgtcga cagccccctg gccgtgaatg ccaccgaaat attcaggatg aacaccgact
     1321 gcctgaacga ggaagtccac gaggtgatga agaccgacgc ggatccgttc ggtttcggat
     1381 cgctctttta cctgaagagt cccgaggagt cgaagaaact gaattcctac aagaaaccct
     1441 gtgtcattat ctcggcatcg ggcatgatgg aagccggacg gatcaagcac catctggcca
     1501 acaacatcga aaatccgaac aataccatac tggccgtcgg atactgtgcc ccggttaccc
     1561 tgggagccag gatcctgaat ggtgagaacc cggtttccat cttcggggaa aagcacccgg
     1621 tgaacgcgac catcgaacgg atagaagcct tttcaggcca cggcgattat aacgagatga
     1681 gaaactacct taagtgccag gatgccggcc aggtaaaaaa agtattcctg gtacacggcg
     1741 attatgaagc gcagcagttt taccagaaaa aactcgaaga aacgggtttt aaaaacatta
     1801 tgatcccgtc gtccggcact gaagtaaaac tctgattccc cgggtcattt ttggcccgac
     1861 tcggtggtaa ttgtgttttt tattttgcat tcctgctttt tttatcgtac ctttaaataa
     1921 aaagagccaa tgccatggaa aagatttccg atttctgcgg ccgggaattg caatggatgc
     1981 aaccccgcat gactgaacct tattttgaac tgaaagccgc tgatgaaaag gtggcctctc
     2041 taaaattcaa aaatgcgttc ggatcattcg ccgaaggcac ttttgccggg ggaatatgga
     2101 cattcaagcg gcagggtttc ctgagttctg ttgtcaccat cagggaaaaa ggatatgaga
     2161 acaaccttgc cgtttaccgg aataatacct ggagcgaagg gggaaccctc gaaataccgg
     2221 acggagtaaa gtataaagtc aattccaatt actggaacga gcagtttgaa tacaccggcg
     2281 agtcaggcaa tcttttgatt caatgcacca agatcagcgg ttttaagctt catgccaggc
     2341 tggaaatatt ccctgatgcc tgcgaactcg acaaataccc ctggatgctg ttgctggggt
     2401 ggtatctggc tgtgatgtcg agccgtgaag gcgaattcat ggccggtctg ttctgagaag
     2461 gatgaatgcc gttttattat tgtttccggc agagtgggtt ttctgatggc gatgagacta
     2521 taagccgttt taaacgatta ttccgttttt taattaaatt tcctttgaac cgctttctca
     2581 acctgttgat ttaggtaact ttgcacttta tttggtgaaa tgagcgaaaa actatacgtg
     2641 tacaatacgc tttcgaagca gaaagagttg tttgtcccta tcaacccgaa ccatgccggc
     2701 atgtatgtat gcggccctac cgtttacggc gatccgcacc tcggccatgc ccgcccggcc
     2761 attacctttg acctggtttt caggtatctg cagcatctgg gttataaagt ccggtatgta
     2821 aggaacatta cggatgtcgg ccacctggaa aatgatgccg acgaagggga agataagatt
     2881 gcaaaaaaag cccgcctcga agaacttgaa cccatggaag tggtccagta ttacagcgac
     2941 cgttaccatg agtatatgga ccgccttaat gttcgcaaac ccagcattga acccagggct
     3001 tcgggccaca tcatcgaaca gataagcatg atcaggaagc tggtcgacag cggatatgca
     3061 tacgagagca atggttcagt ttattttgat gttgaaaagt acaaccagac cgaaaaatac
     3121 ggcatccttt cgggacggaa actcgaagac ctgctgacca acacccgcga gctggaaggt
     3181 caggatgaaa agcgcaatcc ttttgatttt gcgttgtgga aaaaagcgca gccggaacat
     3241 attatgcgct ggccttcgga atggagcgac ggcttcccgg gatggcacct cgaatgctcg
     3301 gttatgagca ccaagtacct gggcgaaacc ttcgacattc acggcggcgg gatggacctg
     3361 atgttccccc atcatgaggc cgaaatagcg caaagtacgg catgcaacca caagcaaccc
     3421 gtgaaatact ggatgcacaa caatatgatc accatcaacg gccagaagat gggcaaatcg
     3481 ttgggcaact tcataacgct ggaggagttc ttcaccggat cgcacgcggc gctcgaaaag
     3541 gcatacagcc ccatgaccat ccgttttttc atcctgcagg cccattaccg cagccagctt
     3601 gatttcagca acgaagcctt acaggctgct gaaaaaggtt tcgaccggct gatgaaaagc
     3661 atggaaacac tccggcggat tactccccaa aacacttctg ccgttgatat ccccggtctg
     3721 gagaaacaat gctatgacgc gatgaacgac gacttcaaca gtccgatcct gattgccggc
     3781 ctgttcgaag gtgtccgtat catcaattcg gcttacgacg ggaaggaaaa cctgaccgcc
     3841 gatgatatca aagggcttga gaagatattc aacacttttg tcattgaaat ccttggcctt
     3901 cagccccagg ttgatgtcaa tgcaggaagt aacgttgtgg atggcctgat ggaactgatc
     3961 ctcgaaatcc ggaaaaacgc ccgtgccaat aaggattggg ctaccagcga taaaatacgc
     4021 gacgaccttg ccaggatcaa tatcactgta aaagatacca gggacggagc gacctggagc
     4081 ctggaataac agcctgtttt ccccgtaact ttcaccaaaa ccgataaaaa caatgaaacc
     4141 cgtcaaaaca ctgtctgcct tgctggttat aactttattg atcagcagtt gcgccaacaa
     4201 aaccgaaaaa atggaaaaag aacttcgctc atttattgcc cgctacgatt cggtagtaca
     4261 gccgcttcaa aaggctgcct acctggcctc gtgggatgcc tcaatatcag gcaaacctga
     4321 agatttcaaa aagtcggaag atctctccat tgaacttacc aaagtttacg ccaataagga
     4381 tgattttgcc gtcctgaaaa agatcaagga atcaggcgct gtgaaggatg aaatgctgtc
     4441 gcgccagctt accgttcttt ataatgctta cctgggcgct caggttgata cagccaaact
     4501 caacgctgtt attaagctgc aaagccagat cgaacagaaa tatggaaatt tccgcgccaa
     4561 cgttaacgga aaacaactta ccgacaacga agtggaaaat atcctcaaga cttcggttaa
     4621 caataaagag ctgcaacagg catgggaagg ccataaagcc atcgggcccg tggtagccga
     4681 tgacgtgaag aaactggtta aaatgcgtaa tgagattgcc aaagaactgg gtttcaataa
     4741 ttaccatgaa atgagcctga aactcagcga acaggatcct gaagagattt caaaactttt
     4801 tgatgaactc gataatctta cccgtgaatc gtttgccggc ctgaaggatg aaattgatac
     4861 ctaccttgct gccaggctga aaataaagaa agaagaactg atgccctggg attaccagaa
     4921 ccgtttcttc caggaagctc cggccattta cagcgttgac ctggataaat attacaaagg
     4981 gcagaatatt gaaaaactga ccacggatta ttatgccagc ctcggccttg acattaccgg
     5041 cattgttgcc aacagcgatt tatacgaaaa accgggcaag aaccagcatg ctttctgtac
     5101 cgacattgat aacaaaggcg acgtcagggt tttatgcaac atcgtaccta actataactg
     5161 gatgaatacc atgctgcacg agttcggcca tgctgtttat gataaatata ttgatcagca
     5221 gctcccgttc accctgcgca acccggccca tacctttacc accgaagctg tcgccatgtt
     5281 cttcgggcga atggccagca acgccaattt cctggttgat atgaagctta ccgataccgc
     5341 cgatgcacgc aaggttgcag aagaaagttt caaggtgctt cgtctggaac agcttacctt
     5401 cagccgttgg gcacaggtga tgtacaggtt tgaaaaatcg atgtatgaaa atcccgatca
     5461 ggatttaaac aagttgtggt gggatcttgt tgaaaaatac cagatgctga aacgccccgc
     5521 cggccgcaac caacctgatt gggcaagcaa aattcatatt gccctctatc cctgctatta
     5581 tcacaattat cagcttggag aactgctggc ttcgcagttt aatcattatc tggccgccaa
     5641 cgtcgccaag agtgataata tgcgcatgag ctatttcggc cagaaggaag tggggaaatt
     5701 cttcatcgaa aaagtatttg ccccgggggc acaatatgaa tggaacgata tgattgaaaa
     5761 agctaccggt gaaaaattaa cgccgaaata ctatgccgag cagtttgtaa aataaccgcc
     5821 ggccaaccat attaaaaaac gggaagaaac cgatcttccc gttttttttt gtgttcacag
     5881 cgaccgggtt tactacagga agttttcatt caggaacttc tgaaaatgtt ccttgtatgc
     5941 ttctccgacc gggatatagg ttttaccgaa aacgatcctg aaacgctcaa tggtatcaac
     6001 ccggttcagg ttcacgataa acgaacggtg tacccgcata aactgactgg ccggcagctt
     6061 ttcctcaagg gtctttaatg aatttatcga tagtatcggc cgggcatcgg tccggagaaa
     6121 aatcttgaca tagtccttca tgccttcgat gtacagaaca tcatcataat taatcctgcg
     6181 gattttgtac tccgatttca cgaaaaggaa ctcatcgttt gtctccatca gcgggtaggt
     6241 cttttgctcc agttcgatca ggttgcgggc tttattcgcc gcgatcagga actcatcata
     6301 gccaaaaggc ttcagcaggt aatcgacggc ctgcagcctg aaaccttcga gggcgaactt
     6361 ctcataagcc gtggcaaata ctattttcgg gccgttttgt aaataccttg ccaggtctgt
     6421 cccgatgagg tccggcatct gaatgtccag gaaaacgaga tcggtttcat ggctttcgat
     6481 aaattcgata gcgctgatcg gattgtcgaa ttcgccggca cattcgagga acggtgtttg
     6541 cctgatgtaa cctgaaatca gtttcagggc cagcggttcg tcatcaatag caatggtctg
     6601 aattttcatg gcctatgaaa caggatttat ttgcaattca acgataaagg ccgatccgtc
     6661 gccgacgatc ttcagttcat gctgaccggg gtaaagcagc ctgagcctct ttttaacatt
     6721 ttccagccct atgcctgaag cgccttctgc ctgcacattc cccggaaaca ggctgttgct
     6781 gcatttgaaa tttatacaat cgtccttcac tcccatttcg atgtgaataa acgaaaatgc
     6841 ccggttgctg atgccatgtt tgaatgcgtt ttcaatgaaa ggcaggaata acaagggcga
     6901 tatgctgaga tccggaaaat cttcggggaa ttttacccgg aggtcgactt tcgggctgag
     6961 gcggagtttc atcagttcga gatatacttt cataaaatct atctcacggc ttatgggaac
     7021 gctttcttta tccgtttcat aaagcaggta acgcataagc tttgaaagcc ggagtatggc
     7081 tttctggccg tcgtcgacat tggtttgcac cagcgagtaa atattgttga gcgtattgaa
     7141 aaagaaatga ggattgatct ggtttttcag gaaagccagt tcggtgttca gttgttcctt
     7201 ctcagcttct tttcgctgtt tttcctgttc gatgtatagg ccggaaaaac ttaggccaag
     7261 acttatgccg gtaaccagta aagccgtaat aaagaaatta tagaccggga gattcatcgg
     7321 gggagtcggc ttttgtccgg gaggcggcgc cgggaattga tttctcagcc ggggctgttg
     7381 tatcatgggc tgttcctgcc tgaaaccggg ggcgggaaaa cgcatcggcg gctgtcgccg
     7441 gaatgtgctg ctgaccaaaa aggttgtcag cacgatgagc gacaatgccg ctgccagata
     7501 ccatatttta tggttctgaa aaaacagctt cggaatgatt accaggtaat tgatataaaa
     7561 aatggcagcg tataaaagca ggttccggta attatgaagt aagacgtccg tgtcggtttt
     7621 gttgcccgaa aggtagaaat atgtggtcag gataaagata acagaccaca cgaacccgtg
     7681 taacagataa ggaagcagct ttttacccgg aaggggcatt tttatatgct tgatatcttt
     7741 caggtcagcc ataagcggtt aaaaaacgaa ggagtaacct aacccgataa caaaatcagt
     7801 ttcaggatcc gggacgttca tttcgtgctg tatcatgtac caggcatcga tactgtgacg
     7861 gttatagaat ttgtactcca ttcctgctgt ataacgcata cagtcgataa ccggcgagtc
     7921 gtattgttta ccgaccggta tgaacgcttc ggtcgtcagg taaggttcaa atgccttgcc
     7981 ggtatcgaaa cctagtttca ggcgcgtacg ttcataattt tcgggaattt tgccatcttc
     8041 cgaggacaaa atatcggtgt actgtgattg gtacctcgcc ctgaccgata aaatcagggg
     8101 catgaattta tgacggtagc tcaggtcgaa ataataacga tggcgcatac tgtaggaatc
     8161 gtcaatcctt cgcttgctga tgaacctgta attggcagaa accttcaaac cgggggtaat
     8221 actgtaacct aagccgaggt cgctgaaaat ggtgccggct tcggtaatat tttcattcat
     8281 cctgagttct tctgcaaaat acagcgaaaa ctccggcgta agctttttct cgaggttcac
     8341 acccatccat aattgagcat cgttggtttg tgccgagaga tacccgggga tgaacggtaa
     8401 aaacaggatc aacaggagag caaacctatt attcatagta gtacaccttg atatcggctg
     8461 tgtcgcgcag aaaattgatt ttactgatcg taacccggtg tatgtttagc ccggtacgtt
     8521 tttcgaggtc gctgatgagt tgctgccgtt tttcagggac aatcagttcg atgttttcgt
     8581 acctcacata ctggcttgat tcccttttaa agatccacct gcattcgagc agggcggcaa
     8641 aggcaataac aagggcgtca agcaccgcca gttcataata ttccagccgt attgcaccta
     8701 ccagtcctaa cgctatcacg atgaaaaggt aggtcatatc cttaatggag atacctgctg
     8761 tccggtaccg cagcatcgaa aacaccgcga acaagccgaa cgcggctccc atcgaaatct
     8821 tgacctcatt caatacatag gtcaggacaa agatgacaag attgaaaatc aggaaggtaa
     8881 agatataatc ctggttttta taggccgggt agtacaccag aaatatcaaa gccagcacgg
     8941 cagtaatatc aattcccagg ttaatgaaaa acgaccagtt gatctgaatg atatctttat
     9001 ctttcttctt atccgatttt tttgaaccgc cgtcgtcaac ctgggtttcg aaggaagttt
     9061 caaacacttg tgccctcatc cctgcaggag cagcaaacag aaggaaaaag aacaggatga
     9121 ttgcaaactt tgtgtgcgtc atgatacaag tttgttaatg ttcagtaagg tttctttgaa
     9181 ttgattgtac tttattccgt catacatcga aattacgccg atgcagtatt tgctaagccg
     9241 tgcaggattc acatgaagat ggtgaagcct tctgataaac ggcgacactt ccgtttttcc
     9301 ctgttttacc tctgcgatca taaggccatc gtacgatttt tcaagctggt catggatgaa
     9361 gatcaggttg gtgtcgaggg taatccgctc attcattttt ttgcttacca gggtcatcct
     9421 cgaaaatttc acggtaagct tggggcttaa ttcatttgct gtataaggtg aatgcttcag
     9481 caacaatttt tctttttcgc ccgagatcac aggttcattg tccggcaccc tgatgcgggt
     9541 tttgatggtg cgctttttat tgtccttaaa cttgatctcg aagtaatgct ggttcgacgt
     9601 aacataggtc ctgatccgaa ccttgtgcct gttcagtttg ccgttgtgat ggtttacgta
     9661 tgccgaaaac tgatccgtat cgaaataagt ggtcagataa gggtataacc gctgcccgtc
     9721 ggtttcaagg atataatact gatccccgag atcgctcaac acttcaggca gtctgtccag
     9781 gttaaagata aacttggtat ccacccggtt gagcagctta acgctatcca tttgctcaag
     9841 cgaaatggat tgaaaagatg acaggatatc ttcttttaat ttcatggttt cttttaaaaa
     9901 taggtgtaaa cccatttttt agtggtctct gtttgttttg ctgtgttaac ggtcgatcgt
     9961 cctgttttca ggtttttgga atcataagtg taggtcgact ttttgagcac atcccctgaa
    10021 ccgtcgtaag taatctccga atttttctga ccttttgaat tatagctgca gactgtcttt
    10081 ttgatgagct tgtcgccgct gaattccttt tcttccgtca cattattgaa agcatcgtag
    10141 agataggtag tcatgatggt ttttcctttt gcggcctcgt aaacggtttc ggatgaaatg
    10201 cgcttatact tgtcgaaaac cgtaatttcc ttcagcagga tggttccgcc ttcatcatat
    10261 tcgatgtgaa gcgttgtgtt cccgttttta tcgtattctt cgtataacga tttgtaactg
    10321 accgttttgc cgtcttcttt ctttgccgtc cactcggttg aagattttgc cctggctgcc
    10381 gtcagctcct ttttcgattg cccgtatccc tgcaccatac agagacaggt cagcacaatg
    10441 attgccggga atttcatgat gattattttt taataacgat atcggggagc tccaccactt
    10501 cattgttttt cgagatggtt acatcggtga taaccggtat atcggcgctg gaggtacagg
    10561 ttgaatcttt tgaatataca taaatgtgat agtcgccttt acgcaggtat ttaaactcaa
    10621 atgcaccgtt ataagaggtt ttaacccggt cgctgtatga aaaatcatcg ccgtaaataa
    10681 tgtaaacatc ctcatcctgg gcgtaatact cttcctgtaa ataagtaaaa ctgctgttat
    10741 aattttttac ccatactttt ccgcggatcg aagaagtgcc tccttccccc gggtcttttt
    10801 cacaggaagc agctatacag cctatcagca gtacaaatgc cacattcctt atcgtgtttt
    10861 tagcagaagt aatcatggct acaagttttt aatggtcctt ttcgttgccg ctttatttta
    10921 gcgcgtgcaa tagtactaaa ataaatgcca cccttcgccc ctgaatttca ggcaacgcat
    10981 tcatccggtg aatatttcat cacaattcag gcactcgaat tgctcaaagc gggtaaatgt
    11041 tctcccgata gacctggata ttgaatttaa ggaggcaaac ttagcaccgt cattccgaaa
    11101 cggaaaatta aatagataaa taagcggttt ttagggttat agcattgatt ttaaacgatg
    11161 gcgatcagga accggcaggc tggcaatccg ggtccggttg tcttattcag ccggtgaagg
    11221 ttggcctgat ggctacatgc tcaatggcaa cggatgaaag ccagaatatg ttcttcatat
    11281 tcccagcgtg atcggttcat tcctgttccg ggccagccgc cggaaaaata accgcacagg
    11341 cagtaaagtg gccggcaacg aaaacgagcc aaccggaatc cggcgggctc gtatagttta
    11401 cctcaatgaa aaaactgtgt cggaattaat tcaggtttac gaccgaaatt ttccaccgat
    11461 tatcggcctg ctttttaaac aggaaggtgt aatatccttt ggcatgaccg actaccgaat
    11521 cgttttgtac ccaatcgttc gtgtatgaac cgctgatgca tgcaatttca cctgactttt
    11581 cttcattttt attgactata gccaggttac gaagcgattt tgccaccggc cctatccacg
    11641 cctttctgag aagattaata ccggtcatca tggtatcagg caaaataaca acggcgtctg
    11701 gggtaagaca tgcggctatt tgtccggtat cgccggcatg ccaggcctcc atccaggggg
    11761 ccatcaagga atccgtttcc ggtattgcgg cgcttttttc ctgctggttc atacatgcat
    11821 ttgacaggaa caataccggt aatattagta agaggatgtt tgttttcatg gcgtgtatga
    11881 ttgaaagtca acagtgtacc tgacaatact ttattcgatt gaaaaaatgc tgatctcgct
    11941 tccatcggat tgatagaaca tttcggcccc gtcgctgaaa tggggattgt cataaaacag
    12001 cagggctgca tctcccgttc cgcgcacctt cattttatca agatcaattg aggcaaaggt
    12061 tttaaaatcg agtttttcgg aattgccatc ggtaaaaatg gcgtaatcag cataatcatt
    12121 atcggcattc atgtggacac tggccggata agccgataca ttgggcgttt tcagcacacc
    12181 ctcgatggtt gcagtaaggg ggtcaatctt gataatgcct ttactgcagt tcaatatcat
    12241 tttttgcttc tcagggaagt agatgagttt ccaggctttt ccgagttttt cctttgatac
    12301 gggtatgctt tttataacat ttccggtagg agggtcgagg atgaagaagt tttcgtcggt
    12361 ggcacaatac agtttcccgt caatatattc caccccggcc aggtatgaga agttgtcctt
    12421 tgcttttttg ccggcctggt ccccattcca gatttcggcg cccgtctttg catcgtatgc
    12481 aataaggccg tacttatcct tatccaggaa cagtcgctta taaacagttc gtgtcgaatt
    12541 gccggtcctt acctcttcgc gtacaatttt agaggccggg ctgccgtaag ccagcattac
    12601 aacgccgttg gttacgagga gagccggtat tttctgggca tcgggatact ttttcgtttc
    12661 ccagaccagt gttccgttct gggcatcata gcacctcacg gtgtttttaa ctttatcaac
    12721 cacgtatgcc tttccttcgg atacgacagg cagcggggca gggaaaggcg taagggtggt
    12781 tttgctgagc ccgttcttgg tttcgctcga cgaatagtaa gcggtccaga gcttttctcc
    12841 ggtattgaca tcaataacgg tcagaccgtc attggtcttg agcgccggat catgggttac
    12901 gaacagcttt ccgtcggcgt aattcatacg gatcataggc gtgttatcta tccagtcgaa
    12961 aacatagccg atggtatatt tttttcgcca taactccctg ttggtgcctt cttcataagc
    13021 aataaagttc attttccatt ttgggacgtc ggttggttgt ccgcttagag cgcctaaaat
    13081 ttcgaacacc ctgtttgttt cgccctgcaa aataatgtac atgttctttt cggggatgcc
    13141 gatcatggtt gcatgtttac ggtcggccat gtactttttc tgatcaaaat acggatcggt
    13201 ttccgttact tccctgcctt cgcgggtggt aagataaatg ctttcctcct tgccggtaat
    13261 aatcttcaca aggttataat aattcatgat gtagcaatca aactgctttg ctttgccggt
    13321 aagctcaaaa ttcttactcc agatcacttt accggtataa acgtcaacta aatcaacatt
    13381 atccctggcc gggagcagaa cgccgttggt ttcggcgtca aaatagtttt caaaaccttc
    13441 ggataactcg tactttccat aatcggagac atatttgtcg cttttccaga gcagtttgcc
    13501 ggttttcccg tcaaggaaat atttttcagc aatgcctttt ttagtgtcct catcatagag
    13561 caggatcaca ttggcatctt tgttccaaag ctggtattca aatttctggt ttgcaaagtc
    13621 ggttttaaaa ttgatcttcc agctaatttt accggtggcg ccgtcgaaca tcagcatatc
    13681 ggttttgctg gagcaaagga aaaaccggaa atcgccggta tgggcataca taaaatagga
    13741 aggcttgaac ggcagcttca ggctatgcaa ctccctgaac gaaggccctc ctcctgccag
    13801 tacatttccg gaaaaccata agagtaaaat cataaacagt ccgcgtgtga taactgattg
    13861 cattgttttc atcataaaat ttgtttttag gtgattattg tcctacttat aagcccgatt
    13921 tcttgacatc gaatgcaaga tcaaaagtat caggggccaa aatgcggatc aatccccctt
    13981 tcgtgtgatt ttaacggagt tcgctcctga atacaaacaa tatcggaaca ggattcccgc
    14041 gccgggatcc ctgaaagtgg accggaattc aggctcctga atttgttgaa aagtattgga
    14101 gaagggagga gtggcggctt ctgaccggtg ccggtcagct atttattgat ggagcgctaa
    14161 tggttgatac acttcagcaa tacctcggtg cgggtacgaa cctgcagttt ctgatatatt
    14221 ttgtgaatat gggtgcggac ggtttcgata ttgatgaaca atgcgttggc tatttcctta
    14281 tacaggtacc cttttgccag gtacgagagg atttcctctt cgcggggagt gagattggct
    14341 tccggaaact gcgtcagggc cggtttcata aacgtctgca ctactttacg ggctatctcg
    14401 ccggtcatgg gcgaaccacc gttcaccagg tcggtgatgg cttccaggag tttggcaggc
    14461 ggcgtttttt tcaacaggta acctgtggcc ccagccgcca gagatttata gatttcttcc
    14521 gtgttatcga aaaccgtcag catcatcacc tgtacttcag gatgaacact ttttatacat
    14581 accacacatt ctattccgtt cattcccggc aggttaatat ccatcagcac tacgtcggga
    14641 atctccccgg gaagaatttt cagagcttct tcggccgaag gacaggccgc tatgcaatga
    14701 tatcccacgc ttccatcgat cagcatgcgc aatccttccc gaacggctgc atcgtcttct
    14761 acaatcagga tttttatttc cctttccatt ttattctttc atggttgtgc cggccgcaaa
    14821 gataacatga gcaactatct gttgcatcac acgttagtgt tattctgatg ccgggtccag
    14881 ctttaccgta aacctgatct ctgttccttt gtcaggctcc gaaaccaggg tatacgctcc
    14941 gcctatttcc aatattcttt tctgcatgtt caccagtccg tttccggttc caagcctgtt
    15001 gcccggatcg aaacctttgc cattgtcggt gatgccgatt gataaggtgt tttccgcata
    15061 cgaaaggttg agtcggactg ttgttgcgcc ggcatgtttc acgatgttgt gcaatgcttc
    15121 tttcaccgtc aggaagctgt ttcgccgcga ttccgatgtc atcggggcag gcggaatctc
    15181 ggcggggaaa ttgaaaaggc cgtcgatgcc ggcggtttcg aggtacccgg atgcatactg
    15241 cctcaggtgg gacgaaaagc tgtcgagtgt atcatttttg gggttcattg cccagatgat
    15301 ttcactcatt tcatccacca cattgcctga tatttcggat atctgtccga taattttcat
    15361 tgactggtcc gcttcagcct gccggctctt cgcgagttcg ctcagaatac tgatgcgggt
    15421 gaggctggcg cctatctcgt catgcatatc cttggctatt ctgagccttt ccttgttgac
    15481 ggcattttgt ttttcgagat catgcaaccg gtttttgaat cttctgtcct gaaaaccctt
    15541 tacaattaaa atggtgacga agattgtcaa tacagccgac aaaataagaa accacctggt
    15601 cctccagaaa ggaggagtaa tggtgagcgt caacaggcat gttgctttcg actggttccg
    15661 gtaggcattg ctgcatttca caaagagcct gtaatggcca ggcggcagat tccggtaaac
    15721 aaattccggg ttaaccgaca tgttacccca ttctttctga tacccttcga gcagaaacga
    15781 aaacgactgc atctccggat tttcatagtc atcggtaaat actcttccgc tgatatgagg
    15841 cgactgccaa ctgagccgga tttccggatt ctcgggtggt atgccgcctg aatatgctgt
    15901 gtcggcgaca aaaagcgaga tcaggcggac cccgggagcg cgcatctgcg ggcggaaacc
    15961 ggccggatca aagccgttca ggccatttat gccgccgaaa aatatcctgc cgtccgtagt
    16021 tttatggctc gcctggccgt tgaattcaag cgactgaagg ttatttcggg cattgaacca
    16081 tatgatggaa tttctctcct gcggaatgca tccgattccc ctgttaccgg taaaccagat
    16141 acggtttaag gcatccggca ccagggaata gatcacttca ttctgcaatt cggccagggc
    16201 ctttaattcc gggagtctac ggccccgtat atcaaaacgg tcgatgcctg tctccgtcgc
    16261 cgaccagatg caattatcaa gataaagaat atcgtttaca ggtttaaggc ttatgcggtt
    16321 ctttttaatg aaaatcatct ggcggaaaaa accggctgac gctttgctgc ggtacaaccc
    16381 aaaaggagtg gctaaccaat aacattccgc agcctgaatg aattttctga tgttgccgaa
    16441 ttcttttgaa tcgagcaaac gaggcttatc tttcggggca taaaggtaca actggcattc
    16501 tgcatccagg gcaaccgtat cgggagtgac aaacagaaaa gttgccgcgt tggatgtgaa
    16561 tgggtgccgg tacgccaaat ttccaagcgt atcaatcacg aacaggccct gatcggtcac
    16621 agcccagagc ctgctttgcc ggtcgaaggc caggtcgaaa taataaaggt tagccggcag
    16681 ggccgtatgg gcaaggcgcg aaacttttga gaaatcctct gataatttcc aaagtccgtt
    16741 tctgtatgta cctgcccaca gttgcccgtt gcggctttca atggtgcggg taaaaccggt
    16801 tttcgccaga ttgaattgca tcttgccggg ggagtaaacc aatacgccgt ctccgtcggt
    16861 tccaagccag aggtttccct gtttatcctc gaaaaatccg atcaccgggg attgtattgt
    16921 atcggtgtgt tttccctgtc gtgtaataag cctgatttct gatgtatgct tgccggccat
    16981 tcgatagacg gactttttcc ctttatccga tatccagaaa ttgccataac ggtcaataaa
    17041 cattttcccg tccctgaccc ggacagtttc cgagaaaagt tccagccgtc cggaaggaag
    17101 gaaggtgtaa atgtttttcc ccgagagaat atacgttttt ccatccttgc cgttcgcgag
    17161 tccctggcag ccgtccctca taccatgcca ccgggacagg aattcgccag tgcggttttt
    17221 ccggaaattt tcaatgatga ggaatccttt atcattataa accagcaggt ctcccgatgc
    17281 agtctcaacc gcctcttcag ggaaaaaatc cgcaggaggt tccatcttat cccgatagtt
    17341 taatcgtagc cggattattt gttctccctt aatacaacag agctttttat cgggaagcca
    17401 tatcaacgga ttctttttga aagatatttt aagaatcctg ggacagtatc caggtttcct
    17461 gttgactgat ttgaaaatgc cgtttgaagt attgaaattc aggatagagg aattggtcga
    17521 aattaaaaat tcgccaggct tattttcaac aatttccctc atgatgcttt ccgaaccgcc
    17581 tgccgtgccg gagtatggaa ccttgaacga acggaaacgc atgccgtcaa agcactgaag
    17641 cccgtcgccg gtaaccatcc agatgagccc gcggctgtcc tgcagcacct ggcttatcat
    17701 actttgtttt aacccctggt caattccata ggccgtcaca aaaaggccac tgacgccaga
    17761 cgttttttca gcacaagcct gttggtggct cgtaaataat aaagtacaag ctaaacatac
    17821 gattgaacag aacagcttat atggtttccc tgatttcttc atcttatacc aggaggtaaa
    17881 gataagaaac ccttatagtt aataaaaaca tttacccaaa aagaaatttt gaatatggga
    17941 aaccctctaa gtaaggttgt tggaaactat gcctgatcaa taatgacgga tgaatttccg
    18001 aatgacgggg agagaatata aaaacccagg acagcagttg gtggatgcag gattccggag
    18061 aatggatggg tcaatattcc cggataattt ttttcaatca gaccgctttc accaggaaat
    18121 aatttttctt tcctttctgg acaaggatgt aacgatcgtt gagcagcata ccggcattga
    18181 caatcctgtc ttcggctgct ttttccttgt taagctgcac cccgccgtct ttcagcatcc
    18241 ggcgggcttc gcctttactg gggaagaccg atgttttttc ggccaggaaa tcggccatcc
    18301 cgatgccttt ttcaagatcg gccctgctga tctcaaactg gggaacgcct tcaaatacgt
    18361 ttagcaatgt cctttcatcc agatgtttca gggtttcggt agtgcctttg ccgaatagaa
    18421 tttcggatgc ttcgaccgcc tggttatatt cattttcgcc atgaaccctt acggtgatat
    18481 ctctggcaag ggctttctga agaatgcgca ggtgcggagc cgcatcatgt tctttttcca
    18541 tggcttctat ttcttctcgg ctgaaaaggg taaaaatccg gatatacttt ttcgaatctt
    18601 cgtcgctgca attgagccag aactggtaaa aagcgtacgg agaagtcctt tccgggtcga
    18661 gccagatgtt gcctttttcc gtttttccga atttccctcc gtcggccttg gtaatcagcg
    18721 ggcaggtaag ggcaaaagct tcaccgcccg ttttgcgccg gatgagttcc gtgccggtgg
    18781 taatgttgcc ccactggtcg ctgccgccca tctggagccg gcagtttttg gtttcataca
    18841 agtgaaggaa atcaaatccc tgcacaagct ggtaggtaaa ttcggtgaac gaaatgcccg
    18901 tttcgaggcg tttctgtacc gaatccttgg ccatcatgta attcacggta aggtgtttgc
    18961 ctacttcacg gaggaatccc aggaacgaaa attccttcat ccagtcgtag ttgtttacca
    19021 gttcggcggc attgggggct cccgactcaa aatcgaggaa gtgcaggagc tgctttttaa
    19081 tgcattcctg gttatgacga agggtttctt ccgacagtaa attgcgttct tcgctctttc
    19141 ccgacgggtc gccgatcatg ccggtagcgc cgcctatcag ggctagcggg cgatgacccg
    19201 ctatctggaa atgtttcagc atcattactc ccaccaggtg gccgatatgc aaggaatcgg
    19261 cagtgggatc aatcccgacg tatgcggttg ccatgcccat ggaaagcatc tccccggtgc
    19321 cgggcatcat atcctggatc attccgcgcc agcgaagctc ttcaacaaaa ttcataagcg
    19381 ccttattttt cagcaaagtt agaaaatatt cggaagtgtc ccgttgtgac tgtcgcagcg
    19441 atcgacaatg cgtggaaatc gtccttcaat ttttatttgt ttattgcgat atttgtaaga
    19501 aaatgaaaac aggaaaccgc taaacgggtt attcacaata agggtttcga aaatcagtcc
    19561 cggtttcttt caataatatt ggaaatatca atagataatg gaaaaagaga ataccatcag
    19621 ctgcccgaat tgcggagccg agatcaacgt aagcgaaata ctttatcacc aggttcagga
    19681 tcagttaaaa aaggagttcg aggctaaaat agccgagaag gagaaggata tccaaaaaaa
    19741 atcggcctac ctgcaatcgg aatatgaaaa gcttgtaaag gacaaggaga acctgcaggc
    19801 acaggttgat aaggaagtca aggtgagact aggatcggaa aaatcgaaac tcgaggagac
    19861 catccggttg gaagtccgga acgaaacttc cggacagatt caaaccctgc aacaggaact
    19921 tgagcaaaaa tcgaaacagg taagcgagtt caacaaagcg ctggccgaga ttgagaagct
    19981 caaaagggaa aaggaagaac tgcgcgatgc ggttgtcctg gaaaaagaaa aagagttttc
    20041 ggagaagctg aaagatgaga agctgaagat aaagcgccag accgacgaag aagtggcttt
    20101 gaagatcaaa gagctggaaa agcaactcga agaccagaaa cttcttgccg aggagatgag
    20161 acgtaaagcc gaccagggtt ccatgcaact gcagggtgaa gtgcaggaac tggccatcga
    20221 ggaaatactc acttccctgt tcccgttcga cctgatctcg gaagtgggca aaggcgttaa
    20281 aggcgccgat gtgatccaca aggtgagaaa caagctgggc gtggactgcg gtaccattct
    20341 atacgaaagc aaaaggacaa aagctttcag cgccgagtgg gtcaccaagc tgaaagccga
    20401 cgccctgggg accaaagcgg atatctgcat catcgtcacc gagaccctgc ccgacggtat
    20461 cgatcgtatc ggacagaagg acggcgtctg gatatgttcg ttcaacgatt ttaaaggcct
    20521 ggtgctggtt ttgcgcgaaa gcctggtcaa gatcaatgaa gcctatgcat cgcaaaccaa
    20581 taaaggcgaa aagatgcaga tgttatacga ttaccttacc agcaacgagt tcaaaatgca
    20641 gttgggcgcc atcatcgaag gcttcagcga tttgcagaac agctatatcc aggaaaaaag
    20701 ggcgatggaa cgcatctgga aacaacgtga aaagcaactg gagaaagtgt tgttgaatac
    20761 caatcatttc atcggttcca tccaggggat agcgggcagt tccatccccg ggctgaaaca
    20821 gataggcccg tccgacaacc ttttcgaact ggaggaataa ggatttctgc cttaaatgat
    20881 atcaccggat aaatagtttc cggcagtaaa ttctgttatc ggttactact ttcaccaggt
    20941 aaacgcccgg aacaagaggg ccggtgctga atgaatgcag ggcagaaacc gtaaaatcct
    21001 gatccatcac tttttttccg ccgagggtaa ataaggaaat ctctccatgt tttccgagac
    21061 tatttttggt ttcaatggta agttttcctc cagagccgtc ggtataaatt ttaatatcct
    21121 gactttcagc gctgttaatc gctgtgttca gatccagcgg gaattctgcc cggaatagtc
    21181 cgcggccgtg ggaagcggct aatactgtat tgtccgaagt ccgcagcgtc agcatatcta
    21241 ctctcacatt agccatgccc gtagtttggg gttgccagta aacctgggca tcgtgcaggt
    21301 tgctggtcga ccacacgccc aattcggtgg cgataagcgc ctggcggctg ttttgcgggt
    21361 gatagatagc ccatcgcacc ggcatatcgg gaaggttgcc ttccacttcg ttccattgtt
    21421 caccgccgtc gtaagtttgc catatcgaag aaaccccgta attgctgaat gttaccagta
    21481 aggtgtcttc cgatccgccg atcgcgatgc acgaaatatt ggaagcaggg aattcaggac
    21541 tgccgatttc ggtagtttgg ggtaccgcct gtgcattatt tactttaaac agcctgccgc
    21601 tcaccgtccc caggaacagt gtcgtggatg actcgttaga aaagggcgat atttttacat
    21661 gtgaaaagta accttcgcag aaggtgccaa catcaacgaa aacgccgttg ccgttttgag
    21721 gaatatccgg aacgcgtaaa acctggttgg cgtgtgtacc tacaaaatcc accgcattgg
    21781 cgatcagcag gttgttgtta ttctggtaat cggccgggtt gacgaacacg ccgctctggt
    21841 tgtcgatatt actaacccag gtgccattct ggaatactgt gtaccggttg taataataag
    21901 aagtgaggta taggtttggc tggtcctggt cccagaaaca aaaagcgccg tcgccaccgt
    21961 caatcatgtt gctgatcgaa aggggagagc cgttgtaaag caaggttccg ttgtcctgca
    22021 gcccgccgat atattggtcg gttccggctg tgggatggat ggcgcaggta taaaactgta
    22081 atgtattgta attggaattt ttttgttgaa aaaccggagg gctgccgtta gcattagaag
    22141 taaagaaaat gccaccgtca ctgccgaaaa gcgctgtgga tgacgagccg ggtttataga
    22201 ccatgatatg ctgatcggca tggacatagg tatcgccgcc tccgtaatac atcatggccc
    22261 agtcgctgag atgctgccag ttggttccgg aattggtgga acgccacacg tcaaggccgc
    22321 cggtataaat agtttccggg ttgttgggat caacggtaat ggtaagcgcg tgccaggcaa
    22381 ggttagccca gtcgccgctg gtggtcggct tgttgagttg gtcccaggtt gcaccaccgt
    22441 cggttgtttt gatgatatat ctcccgtaat aaacagggtg gccgttggca ttcagatagc
    22501 cggcgccgat ggcggcatat acgatatcgc cgtttgaagg ggcaggcgca agcatcaccc
    22561 tgccgggttt tttatattgc ggttgggcaa tgatcaggtt gctgatatcg tcgtatacaa
    22621 tccaggagcc gacagtgccg ttatcggagg ataggataca ggcgcctcct gcatcattga
    22681 ggttctgcat ggttcctacg aaaatcctgc ccgagggtgt gacggcaatg tcgctcaccg
    22741 catacggaac atcagaaccc gggatcagtg gcagtacctg ttgccaggta gatccgccgt
    22801 cggccgaacg ataaagcccg tcggaaggaa gcgattgctg attggccccg tgataatatc
    22861 ccgatgatac accggcataa attactccgg tgccgttttc gttgcgcact gctatttttg
    22921 tgatgtaggc aaaattgctt gtcgagggaa gcaattccca tgttgcgcct ccatcggcgg
    22981 acttccagat gccaaccccg cggcccgagg attcacggta ggttatcaat gctgtttgta
    23041 cctcaccggt tccggcataa aatacctggg tattcgccgg atcgtaggtt atacagctta
    23101 tagaaagact gctccagaaa tcattgactg gcgaccattg cgataacggg gaagtgacat
    23161 tgttgttgta ccataatccg ccggtaactc cgccggccca ggctttgttt tcagcattcg
    23221 ggtcccacat aatggcccgg gtgcgtcctc ccatctcact cgaagttcct gaccattgca
    23281 actggtatcc gccagctctt gtccggctga gtttttgcag tatccggttg gcttcgataa
    23341 ggcgttcggc cggaacccgc ttttctgccg gatcggccgt cataaaaaat tcctgaaggg
    23401 cagccatatc ggggctgtca gcttcttttt cctttccggg ctccttccct gatttcccaa
    23461 tggaaattgc ggctgtttcc cggaggtatc tctcgtattt cttcctggca cgctcagggc
    23521 ttctgttaac aagcacggct gttaaaaaga atccggccgt aagaaacgct gcaatggcaa
    23581 tcaccatcgt ctttcttgaa aagatatctg atctcatcgg gaagtgtttt tgccaataaa
    23641 gatcgtaaaa tcagggacat catccatata gggcagcaat ataattgatt acctgagaag
    23701 atttttatct ggccggcctg tcctgtttgc cggcggagaa atatcaatgt gcactatgaa
    23761 cgtcaaagga ctatcatttt ttaatttgta aaatttcatg gttcggggat attgttcagg
    23821 ctatggttac agatgccggc aaacattgtt gtaccgggtc ggcagtttaa actgagattt
    23881 atttttacat ggatttaatt atggataata ttgatatatt tgttctaata ttagtggaat
    23941 agcttaattt catcgttctc taataatatt tatgaatgaa gaaattgaaa agtatccata
    24001 atgtgaacag gagttgccga aaatccttta aagagtagga taaattaaat ttaagttcaa
    24061 tgaaaaaagt attgatttcg ttaacggcaa ttgccattgc tgttttttca tctgttaacg
    24121 cgtattctca aagcagtttt tcctttcatg tgggaccggc attcccgatt tctgatttcg
    24181 gaaatgatga tgtcgacgat gacgatgccg gtggggcaaa tgtagggcta aacgttggcg
    24241 ggaaatatat ctatccgctg aataataatg gtttaggtct ttttatcggg gccgatctta
    24301 actataacgg tttaaaaggc agtgtgaagg attatattga agatgagtat cgttacctcg
    24361 gagtcgatga cgaggatatt tcatattaca agtttataaa cgttccggtt actgccgggt
    24421 taagcggcac ctattccggc aatggacaag tatccgtgtt tggtgaagtt ggcataggag
    24481 ttgatttcct gaagattaca gatctgacta ttgaagttaa taataaagat atggtaacaa
    24541 actttgatat gtctgcgcaa ctggcatatg taatcggcgg aggcgtttgg atcaatgata
    24601 aagtcggtat cgggttacat tacaacaacc tggggaaaca tgagattaac ggagaaatga
    24661 aatattccgg atatactgaa gatctggaaa gcagtgattt aaaagtcggt ctggttacgc
    24721 tgacactcgg agtcaaactt taaactatac cggcaattaa cagctctgtg ccctgcagat
    24781 tttgaaggga accagaaaaa aacggttgcg ggcgggtgtc ggaagacacc cgcccgcaac
    24841 cgttatagcg acagaaattt tatattgccc aaaaccggga aattcgctca aaccggaact
    24901 gcatgaaaga tcagaatgtt gggtttccca cacccgccgt catttttccg cccggggaaa
    24961 gtctccggtt caacaactcc cgtttctttt tagcctcccg gcgccggatt tcagaattct
    25021 caggaggctt ccgggcatta gtttcctgtt tccagaaggt cggtgctgaa cgaagcagag
    25081 ataaccatct ctccacttgt gttgtagcct tgatccacac ctttaaattt aatggtccct
    25141 ttcacttttg tatcgcttaa ctctgtaatc acaaccttat attccgggct ggctaccgta
    25201 gatacaaatg cccttgccgt ataatacgag tttggctgca aaattctgta atagcagcac
    25261 gcatattcgt cggggaaggg atatgaaaac ggactggcaa ttgggtattc cttggttgtg
    25321 atgttagggg cgtttacata catatcaaaa caagtcttgt tcaactcggt gataatgctg
    25381 agatgctgtc ggccggagac tgtctggaga gtagctttgg tgatggtgta aacggaatcc
    25441 tttccatcaa aatttgcttt ccattggttt tcacgagccg gttcatcgct gtccttgctg
    25501 caggaacaga taaagccgaa cgcagcaagc agggggatca tcaggaataa tcttttcata
    25561 tcagttaagt tttaaagttc ataatttgcc tgattattca cgcagacatt gcttattaat
    25621 ttaattttat tgcattggtt accgtgaata tcaggcatca ataaagtagc atcagggaag
    25681 tcgcaaaatt gggcaaccat cccatgagtg tcaatcacac tttcgtgtga tttttgagat
    25741 cataaaattt tggatagaag ttagagaagg attacttcca atctttacaa cttgtctgat
    25801 gatccatcat gaagctattg tggcatgcgc ttaccgcttt catgtcgcaa attcaccggt
    25861 tggcgcgctt cctgaagcat gtctgaatta actttcttcc ttgaagaaaa gctggtaaaa
    25921 gtgcatcccc gtttcctcat catagccttt tataattttc tgatgatcgc cgtgtacgta
    25981 tatatggaag ttcctgtcca gtttaatcac gctcctgaat acccttgact gctttttgac
    26041 tgccgcctcc gatatggaaa aattatcatt cagttcaata tccctttcct gctcaaattt
    26101 tgatttatag gatttgaaac tgtcgataac ttccggttca gccagcactt cactggcaaa
    26161 ttcatccatt tcaaagctgt ctttttccct gaaaaacttc accgaccggt tgagcagttc
    26221 ggcctggtcg gccctggtca tttcaaattg ttcgggtagc ttttcaacca cgaagctttt
    26281 tgtgaggctg aggaggtttt tggtctggaa aaagccatcg ttcctgggac gcacatgcag
    26341 gaaacgatcg gtccagtatt gggcgtcgtt gcctttgccg gtattatcca ccacgcttac
    26401 caggtatcct ttttcctttt cggaattgaa aatcaggcag cctttgtcca gcctgttgat
    26461 gttgatgccg gtttctgcat caatggtaaa gccgtcctgc tcctggtaaa ctttcaggaa
    26521 agtatccttg ttttccgact tgaacaagcc gacagcatca acggtctcgc cttcgatgat
    26581 gcaatcgctg aagtacacaa cgtaaaactc accgcccttg attttcggat gaacggactg
    26641 ttcgtacagg tgctttgcca ggttaacgga ttgttcatgc aggcaacccg gatcctcaaa
    26701 aatggccgaa acgtaggtgt aaacttcgtt taagcagata tccgattcgt gataaaggtt
    26761 gtagtattca caatccctga acgaagtcag gaaatagttc tgaagacaat cctttaattc
    26821 tccggtcaat tccagttcgt taccggaaaa acggactcct tcatctcccg acttgttccc
    26881 gacaaattgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tctttccgca
    27001 acagttcctt gaactgttca atctcgtaag catcagccag ccggtacggc ccgatgcggg
    27061 tccggcataa tgccgaaaga taggcgccgc tgcccagcgc ctggccaaaa tcgcgggcca
    27121 gggcgcggat ataggttcct ttgctgcaca caatcctgaa gtcaacttcg ggcatggcca
    27181 gccgggtgat ctcaaattcc ctcacgttta cgctgcgggg ctgcaactcg aggtcggccc
    27241 cctttcgcgc cgatttgtat gcccgtttcc cgttgatctt gatggcggaa aagatgggtg
    27301 gcacctgcat gatatcgccc gtgaggctgc tcacaacatc ggcgatcatg ccgtcggtga
    27361 tatgttctgt gggaaaaacc tggtcaacgg gcttttcgag gtcaaacgaa gcagtggagg
    27421 cgcccagcac aaaagtgccg gtgtattcct tttcaagccc cataaactct tcgatacgct
    27481 tggtagcttt accagtgcaa agtatcatca gcccggtggc aagagggtcg agggtgccgg
    27541 catggccgat cttcactttc ggcatcgcca gttccttttt gaaaaacgac cgcaaagcgc
    27601 cgatcacatc aaaggatgtc cagcggtaag gtttgtttac caggataatt tcaccggtct
    27661 caaaatcgaa agccattata ccaccgacag attaatccct gccagcagaa ggccgataat
    27721 gatcactccg acaatgatgc ggtagtaacc gaacagctta aaaccgtgat cggtcaggaa
    27781 tttgatgaag acttttatgg ctgccatagc aaccaggaac ccgacaatat tcccgagggc
    27841 aagcagccgg atattgtacg atgtcaaaat ctggggcgca tcctgaaaag tatcccacag
    27901 gcttttggca aaagcacctg ccagggtggg tactgcaagg aaaaaggaaa attcggcggc
    27961 agcctgccgg gtgagcttct gctgcatccc gccgataatg gtggcggcgc tccggctcaa
    28021 cccggggaaa atcaccgaca gtatctggaa gagaccgata ataaacgaat tacgatacgt
    28081 catctgctct tcggaacgga tgatgggatt tttgaaccag ttgtctacga ataggagtat
    28141 aatccctcca aggaacatca ccacggcaat gaagagcggt gatccgagcg cacggtcgat
    28201 atgctccttg aggaggatac cgacaatggc tgccgggata aaagcaacga tcagcttcag
    28261 gtaaaagctg aattttttaa aatcaatgaa ttttttccaa tacaggacaa tcaccgagat
    28321 aatcgtcccg aattgtatgt tttcgataaa gagctttgta aatgcttcct tttcaatccc
    28381 catgaatgag gcggtcagcg ccatgtgtcc ggtagacgaa atgggaagga attcggtgat
    28441 gccttcaaca atggctataa tgattgcctg taaccaactc atgctattct tttggtttct
    28501 tcatgatggc aaaaagttca accacgaaac ccgaaagaat caggattggg gacagtgtca
    28561 gccttttgaa attaaacata tcatagttga agacgttcgg gtcttttgat ccgccgccta
    28621 tcatcagaat gaatcccagc ataatcagca gtacgccgat gcccatgata atatagtttt
    28681 cacgtgtaag ggcgaactgg ggaggctttt cgtcaaattt ccgtttagcg ggggtagccg
    28741 gaactacttt tgtttcttta gccattgaat tgttatttaa caggttatga aaattcggtt
    28801 cattgttagc tcatgtgaag gctgtcggct ttcatatgca ggaatttccg aacggcaaaa
    28861 taggtggaaa gccctgaaag aaacacgccg atcatcacca acaatccata catcactgtg
    28921 aaagatcgca tgtcctcaag ttgtatcagt tcgggaatcc tctgtctggc aatccataaa
    28981 gtacccatca ggaatcctat tgccagcagc gaagcgatga ggccatgcaa catgcttcgc
    29041 cggataaaag gccggcggat aaatgattcc gttgctccga ccagttgcat gctcctgatc
    29101 aggaagcgcc gtgaatagat cgaaagctta atggtattgt tgatcaggac aatggcaatg
    29161 ataatgagca acatgctgaa cccggttatc accatgctga ttttccggat gttgtcgttt
    29221 acaacatcaa tcatcgactt ctggtaaacg acttccttaa ccaggggatt ggacttcagt
    29281 tgcttttcga tttttgaaat gctgtcgtta ttggtatatt ccgatttcaa ctgaaggtcg
    29341 atagctgccg gcaacggatt gaaatcaagg aaatggataa aatcctcgcc gagatcctca
    29401 gtaagttctc tggctgcttc ctcccggctg atatattgcg aagaacgcac ataccgggta
    29461 cggtcaagct tttgtttgta cgtcagtatc atcgattcac tgctgtcttc tttcatcagc
    29521 agcgaaatgc ggatattttc gcggacatat tccgaaagtt ttttcccgtg aagaataatc
    29581 aatccgagca atccaagcat gaacagtacc agcataatgc tgataatact cgttacatac
    29641 gaagtgcgaa gccttcgctg tccaaaactc tcgttcgatt gtgaattggt catcggtggc
    29701 aacgaaagta caaaatttta taatcggctc atgaatacca aactgcggcg ttttcgtgta
    29761 aagatgaaaa acggtacttt tgcaccctca ttgtcagcat gaaatccggt gaaacgcctt
    29821 cccggattat gataatttaa caagccaaca taccttttaa gtttttagtg catggattat
    29881 aactttcgcg aagccgaaaa aaaatggcag aaatattggg ccgataataa aatttacaaa
    29941 tcggaaatcg atcattcgaa gccgaagtat tacgtcctcg acatgtttcc atacccgtcg
    30001 ggcgccggcc ttcatgtcgg tcatccgttg gggtatatcg cctccgatat ttttgcacgg
    30061 tataaaaggc tgaaaggtta taatgtgctg catcccatgg gctacgatgc ctatgggctt
    30121 ccggcggaac agtatgccat tcaaacgggc acgcatcctg ccgtcactac cgaaaagaat
    30181 atcaaccggt accgcgaaca actcgataag atcgggtttt cctatgactg gagccgggaa
    30241 gtgaggacca gcgacccgaa atattacaaa tggactcaat ggacttttat aaaactgttc
    30301 cattcctggt tcgaccggca ggaagacaaa gcccggccta tagaaacgct gaccggcatt
    30361 tttgaaacag aaggcaaccg gaatgtaaag gctgcgtgca gcgacggtac gcccttattc
    30421 tcggctcagg aatgggcttc gtacgatgaa aagcagcagc aggaaatcct gatgaaatac
    30481 aggctggctt accaggccga tacggtcgtc aactggtgtc ctgcattggg cactgtgtta
    30541 gccaacgacg aagtaagcga aggccattcc gtccgcggcg gacatcccgt ggaacgaaaa
    30601 aaaatgaggc aatggctgct tcgtatcacc gcatatgctg accgtctgct aaagggtctc
    30661 gataccgttg actggagcga atcgctcaag gaaatccagc gcaactggat cggccgcagc
    30721 gagggtggca atatgaactt tgctgttgaa aacagcgatg ccgtcataga ggtgtttacc
    30781 acacgcccgg atactatttt cggggctacc ttcatggtcc ttgcgccgga acatgaactg
    30841 gtttctgtta ttacaacacc ggagcagaaa gacaagatcg atgcttatgt tacctgggcc
    30901 aaaaaccgtt cggaacgtga gcgcatgacc gaggtgaaaa aggtaagcgg ggaatttacg
    30961 ggcgcttatg cgctgaatcc gttcacgggg acaaaaatcc cggtgtggat tgccgattat
    31021 gtgctgacgg gatacgggac cggcgccatc atggctgttc cggcccacga cagccgtgac
    31081 tttgcctttg cgcggcattt caaccttccg atcatccagg tggtgaataa accgggacat
    31141 gagccttccg atccgtcgac gtgggaagag tcctatgact ctcacgacgg tatcatgatc
    31201 aactcgggat ttatcagcgg catggaggtg aaccaggcta tcacggaagt cattcgaaaa
    31261 gccgaagaga tgaaaatagg gacgggtacg gtcaatttcc gcctccgcga tgccattttc
    31321 agtcggcagc gttactgggg cgagcctttc ccagtgtatt acagggacgg tatgccatac
    31381 accctgcccg aatcgcagct cccgttgctg ctgcccgaag tggatgctta cctgcccacc
    31441 gaaaccggcg aacctccgct ggcaagggct aaaaactggg tgaccgccga cggctgccgc
    31501 ctcgaaacca acaccatgcc cggatttgca ggatcgagcg gttattactt caggtatatg
//
LOCUS       DAOPYV010000033        31472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_1368_length_31472_cov_11.818028, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000033 DAOPYV010000000
VERSION     DAOPYV010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 31472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31472
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1368_length_31472_cov_11.818028"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PKI35_06045"
     rRNA            complement(<1..332)
                     /locus_tag="PKI35_06045"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1231..3726)
                     /locus_tag="PKI35_06050"
     CDS             complement(1231..3726)
                     /locus_tag="PKI35_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX43982.1"
                     /translation="MNRKYLAWLLILTLAAETTFAQVKKADRLYKSYSYALAIPYYLK
                     IAQKPGHKDRDHAIIRLADCYRLTNDQLNAKAWYSRAVNLPSSQDINWFYYGQALRCT
                     QDYDMAREAYEKYAALVPDDPRGKAYAGFCTQIDKLNDIPAAFEIKNANNLNSVRSDF
                     GPAFYGDGIVYASDRRQNYMDDKRYEWTNFNYLDLFFSSPKYLDQFFQDMSDPKSFSG
                     KFNQTYHDGPATFACHDSLMYFSRTEIGNEKRDEEHFRTLKLKIFWAQNKESWSTPEP
                     FFANSNSYSVGHPSLTPDGKTLYFVSDMPGGFGGTDIYCCNWQENRWSEPVNLGEKVN
                     SFGNEMFPFIDGDTLYFASDGFPGFGGLDVFRTSLSNGNWSVPENLGAPVNSSFDDFS
                     FILDKTGKAGFFASARPGGLGSDDIYAFRKLPEKEVRKKKASHALAEGAGITEGDTTS
                     VAEISGIIRHKETLQPLAGSTVFLLNTNTGKVKVIKADENGQFKSLVSRGVMYVAKAM
                     KNNYLSDCLTLNLPLDDTASKVLAPRDFLLDSLSLNKVFRINNMDYQIENIYYDFNKY
                     YIRPDAAAELDKLVQVMKENPVTIELGSHTDCRGSKEYNFDLSQKRAESAVRYIVLQG
                     IEAYRITAKGYGESQPVNHCTDGVYCTPEEHQANRRTEFRVTGYNHSDARNTFDMDKF
                     IDGETIPVYMFDRDFFINCLQDKLSGQTQSQATEASAAPGQEKAISGQDNYVTISPVK
                     NEEAVQPESVPEGVNITYRVQVFALSKEKSLSDPEFYGLDDIMMYRHNGLYKYTSGVF
                     NSLDEAHAYRDLLVSSGFDDAFVVTFVDGNRLE"
     gene            complement(3760..4674)
                     /locus_tag="PKI35_06055"
     CDS             complement(3760..4674)
                     /locus_tag="PKI35_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNX43983.1"
                     /translation="MRKLIITLLLFTALNGMAQQDALFSQYMFNKLTVNPAYAGSREV
                     FSADIISRYQWVGFDGAPNTISVSMHSPLKNDHMGLGCYAYHDKTGAMIDQGFQATYA
                     YRIILPKGKLSLGLSAGFKYNYIDFSKVFVEDPDYYFYGSQNNRFVPDANFGVYYYSD
                     HLYAGISSKQLLQNEMALVETNDGKAYSKLLRHFYGMAGIAIPLSDQVIFRPSTLIKF
                     VINAPWQMDLNASFLINNAFWIGATYRTDNTVVAMTEFMIGKSLRIGYSYDYNFSPLR
                     NCNSGSHEIRLGFDFDLLRIRMLTPRYF"
     gene            complement(4699..13155)
                     /locus_tag="PKI35_06060"
     CDS             complement(4699..13155)
                     /locus_tag="PKI35_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HNX43984.1"
                     /translation="MLIPEKYYLPAIPAQPVITCPGNLTTVTDPGFCTANVTAGLSAI
                     YNDPDGDITTLTWTITGATTGSSPLTGINNLTSYVFHAGINTVTYTLTDDGGLSDSCS
                     FTVTVNDNQPPVITCPLNIRATYDEGNCDAMVNIGTPVAVDSCGIASVVGTRSDGLLL
                     SDAYPAGLTTITWVATDVNGLTATCTQSVYVTEATLLTRYNFVGAAAYPASPTQTAGG
                     ITCEATSSEPFNILGTTGTAAGSLAFVSDSAANPAIFMDPSTGTNTRYFQFHVGGDSL
                     YKYRRFKLYVQARRGNRAAQAINFSYSTDPTSYTINGSMSLLLSNTWYEKVLDWSSVS
                     LINNPSNLYIRLFASNGTGGAGDGRLFIDNFQLTGVDGPLARPNSATIPENTSVTLPI
                     LNNDYFPCHGPATVNPVSFVELPSLGTAILNADNTVTYTPNPNVNGDDNFVYQICDSY
                     GSCDTAIVRIHINAVNYAPAITCPGPVVNSGSDVGICGAIINNIAATYNDPDNNIISL
                     TWIMAGATVDTSSSTGINNILNPYLFNYGATAVTYTVTDADGLSATCSFTVNIEDSEY
                     PSIICPSDTIITIPSCETFAGGVILTEPIVDDNCDTLSLINDAPVQFPLGDTPVIWTV
                     TDIHGLSSQCAQIVSVVQDLPMILNMAGTPVTCYGRNDGTATVTVTNGAAPYTYSWNT
                     VPPQNTATAINLTAGNYTVTVYDTNGCYAIDSITITQPASALIASITDISNVLCYGDE
                     TGSVTAVASGGTPPYRFSIDNIIFQAGNTFSGLAAGSYTLYITDNNGCDTALPFLITQ
                     PALPLTVALTGQTNNLCFGQASGSATVLAGGGTPPYTYVWNTVPPQYTATASGLSSGT
                     YTVSVDDDNNCGPETQTVTITEPSPLIADVVFTPILCPGGTATVTINAAGGTAPYTYT
                     FNGITQVGNGVFTGIPASAGYLWNVFDTNHCDTISGLLSVMEPDTLEAFVPTPPVSCS
                     GSTATVTIMASGGTLPYTYTFNGVIQVGNNVFTGIPAGTGYIWSVTDANACSTVTDTL
                     DIIESDILSATAIITAPISCAGETATVTISVSGGTPPYTYTFNGITQSGDSVFTGIPA
                     GTGYLWSVTDANGCDAPASPLDITEPLPVTATASVTAPVICNGGTATVTIHVSGGTPP
                     YTYTFDGATQTADSVFTGIPAGIGYTWSVTDANGCGPVAGTPLDVTEPPVPTAFAAVT
                     SPILCYGGTATISIFPINGVPPYTCTFNGVTQVGNYIFNGIPAGPGYVWSVTDANGCP
                     AAPQPPLPVTEPPQITVTASVTVPPTCSGGTATVTIVANGGTPPYTYTFNGVTQTGNG
                     VFTGIPAGTGYIASVTDDNDCGPVNDTIDVTSPDAPVASASVTSPILCNGETATVTIS
                     VSGGTPPYTYTFNGITQSGDSVFTGIPAGTGYLWSVTDANGCDAPASPLDITEPLPVT
                     ATASVTAPVICNGGTATVTIHVSGGTPPYTYTFDGATQTADSVFTGIPAGIGYTWSVT
                     DANGCGPVAGTPLDVTEPPVPTAFAAVTSPILCYGGTATISIFPINGVPPYTCTFNGV
                     TQVGNYIFNGIPAGPGYVWSVTDANGCPAAPQPPLPVTEPPLLIALITSQGNSCTGSS
                     SGYATVTPTGGTPDYTYLWNSTPTQTTQTAIDLPSGTYTVIVTDANGCSDTASVTILD
                     IEPITAFAGPDQNLCNADVTFLIGNTPDPGIGSWSLLSGPNTPSVFPSTGSVAVVSGL
                     IASPTPYYFIYSIDNNGCVSTDTMMVINFNPPTPAYAGNDQEFCDATGNVTTLLAGNV
                     PVFGTGTWTQLSGPNTATIADPSDPNTAVSGLTFGIYAFEWTIVNGNCQVSADVMNAF
                     INAPATAYAGLDDTICEGSMVLLSGASASDYSSLLWTSSGTGIFSDPTIINPEYTPSE
                     TDILNGSVALTLHAIANEPCPEASDQIIITINQSALVDAGPDAPVCQNSAFTVTGATA
                     QNCIAYNWITTGAGTLTGSSTLTPTYTPSAGESGVILLILSGTAAYPCENSADTMQLQ
                     INLPGTVYAGNDIPVCEGSTAILTSSFASNYSSLLWTTSGTGTFNDPTLLHPVYSPSE
                     TDIINGSAILTLHLAAIAPCPDNSDQMVLTITKSPVADAGPDDNACQDWPFTVTGATA
                     ENCAAYNWTTTGTGTLTGETTLTPVYTPASGETGIILLILNGIAYYPCNNVSDTMTLQ
                     INSTALVSAGPDTAVCQGEQFHIIGSTAANYSSLTWSTSGTGTFDNPNILDPVYTPSP
                     TDIYNGYVFLSLTAGGNAPCPDRSDVMKLTLNKSATASAGPDETICEGNSFTVTEASA
                     QMAGSLFWTTSGAGTLIDFTTLTPTYTPAAGETGDIFLILTATGLECGSVSDTMILAV
                     NSKASAYAGPDLTTCNVTPVPVIDALAQNYISLHWSSNGSGSFSDPSLLNPVYTPSVA
                     DANLGMVVLTLNVTGNTGCADSSDQAILTLVEMPVAGAGPDATVCQDESFTVSGAIAA
                     SYSSVLWSVSPPSAGILINADSMTPTFVPASGFSGIATLILNVQGNLACGSVIISDEM
                     FITVNSAPVVNAGPDQTIQPGASTVLAGSATGGSGFYGWNWQPDQMVMNPNVPNPVTL
                     PLYSDTTFTLSVLDLLTGCMASDQTDVFISASGGGIKAVADYDTTLVNTPTTVAVLTN
                     DINPGGVPLTISFCSFPSHGIVIINSDNTITYSPYPDFEGDDSFCYEICADDQSGICS
                     DTLVFIHVKQPDINDLFIFNGLSPNSDGINDKWKIRGIENYPENSVTIYNRWGDVIRK
                     IGDYNNTTRSWDGRNENGELMPNGTYFYILEIENSGTRKGWILMRGEE"
     gene            13892..14464
                     /locus_tag="PKI35_06065"
     CDS             13892..14464
                     /locus_tag="PKI35_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43985.1"
                     /translation="MKFKNSNILVIVLLIFCLGAGYNHTCAQDDYQGSLKGAPLKERL
                     FYGGGLGLQVGSITLIDVSPMIGYKLSNRIGIGVSPTYKYYHWQDYFGKDLDLKTNVF
                     GGSVFARCYVYQGLFAQSEYEYLRYKYDNPASSGKFTNDIWSLFIGGGYSQPLGGRAS
                     MYIMALWNLHDTQDSPYNNPLIRIGMSLGF"
     gene            complement(14540..16006)
                     /locus_tag="PKI35_06070"
     CDS             complement(14540..16006)
                     /locus_tag="PKI35_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNX43986.1"
                     /translation="MNDVFKGLQPERVWHYFAETCKVPRPSKKETQITQYIRQVAGAL
                     SLENYMDETGNVIVKKPATPGYENRCSVVLQCHVDMVCEKNSDTYHDFDHDAIQPFID
                     GEWVKARGTTLGADDGIGIAVQLALMEDKTVKHGPLEFLFTVDEETGLTGAYGLKPGF
                     FDSKILLNLDSEDEGELFIGCAGGKDTIISMNYSKVPVNYDSVAFTIRVSGLKGGHSG
                     DDINKGLGNANKIINRLLWNTAQKYETRISDLKGGNLRNAIAREAFALITLPKRFQND
                     FAGYVRDFNNMVKKELKTTEPELLVSYAPAELPSYVIDLSSQERLLNALYACPHGVIT
                     MSADIPNFVDTSTNLASVKINDKNIEITTSQRSSSESGKEDVVSMVASVFRLAGAKVT
                     HGDGYPGWTPNPDSRILEITRKAYTDLFHSDPKVLAIHAGLECGLFGEKYTGLDMISY
                     GPTIKGAHSPDERLLIATVLRFWDLTIEILRNIPEKTA"
     gene            complement(16171..16821)
                     /gene="rpe"
                     /locus_tag="PKI35_06075"
     CDS             complement(16171..16821)
                     /gene="rpe"
                     /locus_tag="PKI35_06075"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNX43987.1"
                     /translation="MPLISPSILTADFTNLASAVEMINNSEADWVHVDVMDGVFVPNI
                     TFGMPVIKAVKKISQKPLDVHLMIVEPDRHFTAFRDAGADILTVHYEACIHLHRSIQQ
                     IKELGMKAGVVLNPHTPVNVLTDILPDVDMVLLMTVNPGFGGQKFIENSYRKIRELKK
                     MILDVNPDVLIEVDGGVVLSNAQKLAEAGVDVLVAGNTVFSSADPKDTIHRLKHSI"
     gene            complement(17094..17957)
                     /locus_tag="PKI35_06080"
     CDS             complement(17094..17957)
                     /locus_tag="PKI35_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX43988.1"
                     /translation="MRQLKITKQVTNRETASLDKYLQEIGKVELITAEEEVMLAQRIK
                     QGDRAALEKLTKANLRFVVSVSKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKIGAINKINKAYAKLEQEFEREPNSDEIAT
                     MLEISENEVKESMRNSGRHVSMDAPLVQDEDNTMYDVLRSDDGPTPETGLLYESLRKE
                     IDRAISTLTTRESEVIKYYFGLNGSHPLTLEEIGEKFDLTRERVRQIKEKAIRRLKHT
                     SRSKILKTYLG"
     gene            complement(18069..20210)
                     /locus_tag="PKI35_06085"
     CDS             complement(18069..20210)
                     /locus_tag="PKI35_06085"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNX43989.1"
                     /translation="MMEGIVKTFDIGDGRLCSLETGKLAKQADGSCVVKMGNTMLLAT
                     VVAAKEAKEDVDFMPLSVEYREKYAASGRFPGGFFKREARPSDHEILISRLVDRALRP
                     LFPDDYHAEVQVIITLISADTNILPDSLACLAASTALAVSDIPFNGPVSEVRVARIND
                     KYIINPPAADLPAAEIDIIVAATMDNIMMVEGEMKEVSEEAMLQALKTAHEHIKLACK
                     AQLELGSMVEKSSPKQEYCHETHDEELRQALTKFCYDKIYAVASSGLGKKERTEGFES
                     VKKEFIETLTEEEASEKAFLVNRYYHDVEKKAMRDMILNDRKRLDGRKLDQIRPIWSE
                     VDYLPAAHGSAIFTRGETQSLTTVTLGTKLDEQVIDGAVIEEKQNFILHYNFPPFATG
                     EIKPIRGVGRREIGHGNLALRALKAMMPDAETNPYTVRVVSDILESNGSSSMATVCAG
                     TLALLDAGVKLVKPVAGIAMGLITDGEKYAVLSDILGDEDHLGDMDFKVTGTADGITA
                     CQMDIKIDGLRYDILAEALEQARKGRLHILGEMAKTITKPREDYKPHVPRIVKMTIPR
                     EFIGAVIGPGGKIIQEMQRETNSTIVIEEVGELGVIDIVSSNLESIEAVKAKIKKIVA
                     VPEIGEVYHGTVKTITTFGAFVEILPGKDGLLHISEIEWRRLKDVESVLKVGDEIDVK
                     LIDIDSKTGKLKLSRKVLLPKPEGEEKNNQA"
     gene            complement(20446..20715)
                     /gene="rpsO"
                     /locus_tag="PKI35_06090"
     CDS             complement(20446..20715)
                     /gene="rpsO"
                     /locus_tag="PKI35_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNX43990.1"
                     /translation="MYLTPEIKKDLFKDNGKSDADTGSAEGQIALFTYRIKHLTEHLK
                     SNRKDLATQRSLMRLVGKRRRMLDYLKEKDIERYRAIIAKLSLRK"
     gene            21016..21576
                     /gene="idi"
                     /locus_tag="PKI35_06095"
     CDS             21016..21576
                     /gene="idi"
                     /locus_tag="PKI35_06095"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNX43991.1"
                     /translation="MKTDLADMMTTTENQVILVDGSDNALAAMPKLKAHEEGALHRAV
                     SVIIMNSRNEMLLQQRAYTKYHSSGLWSNTACSHPCPGESAHRAATRRLQEEMGITCR
                     LKKVFDFTYRAQFANGLTEHEFDHVFFGRYDGDPMINPVEVHDFKWMPVQDILNGLRA
                     NPQTYTVWFRIMMEQIANRPELFSGR"
     gene            complement(21585..22853)
                     /locus_tag="PKI35_06100"
     CDS             complement(21585..22853)
                     /locus_tag="PKI35_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNX43992.1"
                     /translation="MFQHLTEIITKWLTRAGMQEATAMKIAGVNDFIIVLAIACLLYY
                     LAKFIIIRVVRRIARRTKSMWDDALLENKVFNRMAFLIPGIIVYQSIPSTLSEFSHST
                     ISMAIKFTNVYIIMVFLLVINSFFNAVYAICQDTDYARYHPVKGFIQVGKIIVFTIGA
                     LLILSYFFNQSPWVMLTGLGAFSAVLLLVFKDPILGFVGGIQLSANDMVRQGDWINMP
                     KFGADGTVLEISLTTVKVQNWDNTITTLPTYSLVSESFQNYRGMTESGARRMKRSINI
                     DMASVKFCTPEMLERFRKIDILKDYIDKKQSELLQYNAENNIDGSVPVNGRRQTNIGV
                     FRAYLGAYLHAHPQVHKDFDLLVRQLQPTSSGIPVEIYAFTTETQWLSYENVQSDIFD
                     HIMAIVPEFGLDVFQSPSGNDLRQGFSARS"
     gene            complement(23091..23432)
                     /locus_tag="PKI35_06105"
     CDS             complement(23091..23432)
                     /locus_tag="PKI35_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNX43993.1"
                     /translation="MPKKSRPTGVVYSTNPDFEYRYNEIPETETLPPRMQNLRVTLDR
                     KQRGGKQVTLITGFIGQPEDLETLGKLLKTKCGTGGAVKDGEILIQGDFRAKILEILT
                     AAGYKVKQAGG"
     gene            complement(23476..24366)
                     /locus_tag="PKI35_06110"
     CDS             complement(23476..24366)
                     /locus_tag="PKI35_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNX43994.1"
                     /translation="MAGEKKKVLFIINPKAGITPKIEALLRLHVSLLFGNGNETSIVF
                     TKYPHHATELSEKAIEDHYDIIVAGGGDGTINEVATALFGTGVPMGILPFGSGNGLAR
                     HLGIPLNLIHAMRVIARGNTRLIDTVQLNDRIFTSIAGIGFDAVVAEKFDKSDVRGLL
                     SYTAIILKDYRRYTCNKYRLLIDGQSIEREALMISFANSNQFGFHARIAPQASSCDGW
                     LDICIIRKPSLIKAIFFAAPRIFFGTLGKTNYAEYFRARTVEIPGTAGSVVNIDGEPA
                     RIDNDLKLEVKPLTLNVIVP"
     gene            complement(24370..26325)
                     /locus_tag="PKI35_06115"
     CDS             complement(24370..26325)
                     /locus_tag="PKI35_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNX43995.1"
                     /translation="MKTNTPKPLIQGNPQIVLFSRLFAILLLMMASRMLLFFLNKPLF
                     PDVPASRVAYYALTGLRFDASALLYINLPYILLMLLPFRFRMNRSYQRIAGSIFYVTN
                     AIGFIPNFVDTVYFPFTMKRLTADIFRYITLGNDTAHMMPQFLHDFWYVLLLWIVFVV
                     LMVLICSRIVVFSRKPVQGKGWYITGQTLITVIMAGLTVLGMRGGVQLKPIGIMTAGS
                     YAPVQQTPLVLNSAFTILRTLEQNGLQKKEYFSDEKQMLGFFNASKQYPRSDSTGANR
                     PMDRRNVVIIILESFSKEHSGSLNPKLENGRYLGFTPFLDSLMEHSLVLDGYANGKRS
                     IEGIPAILASLPTWMTEDFITSSYAGNTFNSLAGLLKPEGYYSAFFHGGNNGTMGFDA
                     FCRSAGFDAYFGRNEYGNDGDYDGKWGIWDEPFLQFMAGKLNAMPQPFLASVFTLSSH
                     HPYKIPEKYKGKFRKGKLPIQQSVMYSDYALKRFFETAKKMPWFSNTLFVITADHTSE
                     SAEPFYQSRAGQYSIPIIFYQQDSVLNTNSNITAQQTDIMPSILQYLGYTKPFIAFGN
                     SVFDVTASHAGISSINGSYQIIKDGWSLEWDGISQTQLYDFKSDSLLMKNLANVNLKQ
                     RTAMENLLKSYIQQYNNRMISNSLIIK"
     gene            complement(26397..26711)
                     /locus_tag="PKI35_06120"
     CDS             complement(26397..26711)
                     /locus_tag="PKI35_06120"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HNX43996.1"
                     /translation="MKTIKLIFFILFLAAFSGCKKDGAPPVITILGKNPATTGVGLSY
                     HDAGASAYDEEDGDITSKIIVDSQVDTTKPGTCYVYYNVTDSDGNKANEVNREVIVKY
                     FK"
     gene            complement(26807..27715)
                     /locus_tag="PKI35_06125"
     CDS             complement(26807..27715)
                     /locus_tag="PKI35_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43997.1"
                     /translation="MKKISLIAVLFLVMAGVSFFSACTPDEKEDLSPTLSFVTGSGLI
                     SGDATLKAGEGFTVNISAAANSSSGAKLTNLKVVRTLGGNEVVVTDETIDQSSFTGEI
                     TANAAFIAGSETWSFKVTDANGEYAEISFIITTTAGGEINTFDQKILGSYYNNTYGSF
                     FGSADGTVYKLVDAYNNQAKIDWCYYYGSEIISDHGATLAAPNDPTAMNNVYTNPTYG
                     LSQWSVRNATTFFIVPDQILWDNITDDAMIIAYASATNDTYARELAVGKIVAFKTASG
                     KLGLIRVTDIQTGSQGSITYNVKVQK"
     gene            28010..28177
                     /locus_tag="PKI35_06130"
     CDS             28010..28177
                     /locus_tag="PKI35_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX43998.1"
                     /translation="MKKLFLFFAVAGLISFAACKSSENKTEATADTTAQVETTVDTTA
                     AAPVADTTVAK"
     gene            28464..29699
                     /locus_tag="PKI35_06135"
     CDS             28464..29699
                     /locus_tag="PKI35_06135"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNX43999.1"
                     /translation="MDFNAEKSVTVGNKGKGIRSDCHITLELTNEGGRQIAIESKVQV
                     LYGDDIRRQVNEMLDYFEIGHVKILIEDSGALSYILAARLEAAVRMVKPSVKEYLLPM
                     LPENQYHTSREKNRFSRLYLPGNTPSLAINAGIHKPDGIILDLEDAVAPARKFEARYL
                     VRNTLRSVDFYGAERMVRINQVPAGLDDLEYVIPHNVNLLLVPKCENTEQLRLVNERV
                     AAIKARTGNKEPVWLMPIIESALGVMKAFEIAQCENVVSLAIGLEDYTADLGVKRTQE
                     GNESFFARCMLVNACKAAGIQAIDSVYSDVGNMEGLKENVLRSKALGFNGMGCIHPRQ
                     IKVIHENFAPDKDEIEKAKKIYLAFQDATEKGLGVVSLGTKMIDPPVVKRAVNTIELA
                     LKTGKLPVDWKEQMLTEKA"
     gene            29702..31264
                     /gene="citF"
                     /locus_tag="PKI35_06140"
     CDS             29702..31264
                     /gene="citF"
                     /locus_tag="PKI35_06140"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HNX44000.1"
                     /translation="MKKCDNFVINAAGRKVPTLVNGQEVVAFKGLRQHRPAGRKYASP
                     IPTCIDYPDDGNKIVPSLAEALKKCGLKDGMTISTHHHLRDGDLVSNMIFDIAHDMGV
                     RDLVWLPSASFPCNDPLIKYLEDGTINRIEGSMNGPLGKFTTLGGMKGCAVLRSHGGR
                     VQAIQDGEVHIDIAVIAAPTADAFGNANALNGKSACGILGYALADYMYADKVIIVTDN
                     LIPFPCMPMQVQGNYVDYVVQVDQIGIPEKIVSGTTEVTKSPDRLLLAEWTARFCDEA
                     GLLHDGCSIQSGAGGTSLSVGIYFEEMLKARGQKARFAFGGSTKYLVRMLENGVLDYI
                     LDAQAFDLEAVRSARENPNHIDLSVLQAYNYHSKGNYTSMTDIMILGATEIDTNFNGN
                     VVTHSDGLLLHGIGGWQNCLHARNVIIPIPLFRDRIPVLVDNVTTLCGPGELIDVIVT
                     ERGIAINPKRTDLIEKMKGSSLPIKTIQELKEEAEKICGKPARPRFTDEPVAVIKWVD
                     GTVIDTVWKLEK"
     gene            31246..>31472
                     /locus_tag="PKI35_06145"
     CDS             31246..>31472
                     /locus_tag="PKI35_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44001.1"
                     /translation="METGEVKGQKFTCLGEVVGKMAGLPAWMRLWVRRQGKGQRAKGK
                     GHGAGSITEDGELYLPCARLRVAGRGGRRTE"
BASE COUNT         8048 a         6942 c         7998 g         8484 t
ORIGIN      
        1 tccgtgtctc agtaccagtg tgggggataa tcctctcaga acccctagac atcgttgcct
       61 tggtgagccg ttacctcacc aactagctaa tgtcacgcat gcccatctat aaccgccgga
      121 gctttaactg cattgtgatg ccacatcgca gtgttatggg atattagcca caatttctca
      181 tggttgtgtc ccggttaaag gtaggttgca tacgcgttac gcacccgtgc gctactctca
      241 tccaggtatt gctacccgaa atcccgtaca acttgcatgt attaagcctg ccgctagcgt
      301 tcatcctgag ccaggatcaa actctccatt gtaagaatga tctttaatgt ctcaagattt
      361 attcctcact tctcaattaa ttgaaaaggg atattcgcta ctgtgattgt ttttagcttc
      421 agtctttcaa agaacttttg atctatattt tcccttccgg gaatgatctt gcgtgtttct
      481 tttgttcctt ccttctttcc ttccctgctt tatctttgcc ttgaatttgg gagtgcaaag
      541 gtaaaacttt tttctattct acaaaccttt ctggaaaaaa gtttttcaca atttttttaa
      601 gaacgtgttt cgttattgaa acgggctgca aaggtaaatc atattttgac atctccaaac
      661 aatttttaaa aaaaatctga tttttttaaa actaccagaa ctctcttatt tttcgctttc
      721 ctgctcctgt ctggtgaatg gctcatgcaa cctgttaaag aacgtgtctc tcaaaacgag
      781 ggtgcaaagg taaatcttaa tttaacatct caaagcattt ttgaaaaaaa atgtgatttt
      841 ttttcgatcc tgccaatttt caacatgtta cgttttctta ctcctgcctg atgaatcact
      901 tgtgcaacct gttaaagaac gtgtctctca aaacgagggt gcaaaagtag aaattagttt
      961 gaatatacaa tacctgttac aaaaataatt ctgaaataat ttgaatggat atctataaat
     1021 aaaagtgaat caaaataata aaccggataa attttctgtt caggaagacg aaatatgtgt
     1081 cgagctgatg tattttcatt aaaaatgact gtataaagca ataaaagagg ggtgttaatc
     1141 ctcttttact gtttcgcaac tcagtttctg cgatgatatg gttactgcaa aaatccccga
     1201 tacaggaaca gggacggctc tgattattat ttattcaagc cggttgccat caacaaaagt
     1261 aacaacaaaa gcatcatcaa atcctgaact gacaagcaga tcgcggtaag catgggcttc
     1321 atccaggctg ttaaatacgc cggaagtgta tttatacaaa ccgttatggc gatacatcat
     1381 aatgtcatcc aagccgtaaa actccggatc ggacaacgat ttttcctttg atagggcgaa
     1441 aacctgaacc cgatatgtga tattgacacc ctcagggaca ctttccggct gtacagcctc
     1501 ttcattctta acaggagaaa tggtcacata attgtcctgg ccgctgatgg ccttttcctg
     1561 gcctggagcc gcagacgcct ccgtagcctg tgattgagtt tgccctgaaa gtttatcctg
     1621 caggcagttg atgaagaaat ccctgtcaaa catgtataca gggatagttt cgccatcaat
     1681 gaatttatcc atatcaaatg tattccttgc gtcggaatgg ttatagcccg ttaccctgaa
     1741 ttcggtcctt cggttggcct ggtgttcttc gggagtgcaa taaacgccgt cggtgcaatg
     1801 gttgaccggt tgcgactctc cataaccttt tgccgttata cggtatgctt caataccctg
     1861 caatacaatg tagcggacgg cggactcagc acgtttctgt gataaatcga aattgtattc
     1921 tttgctgcca cggcagtcgg tatgcgatcc cagttcaatc gtaacaggat tttccttcat
     1981 aacttgcact aacttatcta gttcggcagc tgcgtcaggc cttatatagt atttgttgaa
     2041 atcataatag atgttttcaa tctgataatc catattatta attctgaaga ctttgttcag
     2101 agaaaggctg tcaagcagga agtcgcgggg agccaacacc tttgaagctg tatcatctaa
     2161 aggcaggttg agagtgaggc aatcggaaag gtaattattc ttcatggctt tcgctacata
     2221 catgactccg cgtgaaacaa gagatttaaa ttggccgttt tcgtcagcct ttatgacttt
     2281 aactttaccg gtattggtgt tcagcaggaa aactgtagag ccggccagcg gctgaagcgt
     2341 ttctttatgc ctgattatcc ccgatatttc cgcaacagag gttgtatctc cttcggtgat
     2401 gccggcgcct tcagccaggg cgtgtgatgc ctttttcttc cggacttcct tttcgggcaa
     2461 ttttctgaat gcataaatat catcgcttcc taatccaccg gggcgggctg aagcaaagaa
     2521 tcccgccttg cctgttttat caagtataaa ggaaaaatca tcaaaagatg agttgacagg
     2581 ggccccgaga ttttccggca ccgaccagtt cccgttggac agtgatgtac gaaacacatc
     2641 aaggcctcca aatccgggga agccatcgga agcaaagtag agcgtatcac catcaatgaa
     2701 agggaacatt tcattcccga atgagtttac tttctcaccg agattaacag gctcactcca
     2761 tctgttttcc tgccagttgc agcaataaat gtctgttccc ccgaatccgc cgggcatatc
     2821 cgaaacaaaa tacaaggttt ttccgtcggg agtaagtgat ggatgaccta ccgaatagct
     2881 gtttgaattg gcaaagaatg gttcgggagt actccatgat tccttgttct gagcccagaa
     2941 aattttcagt ttcagggtgc ggaaatgttc ctcgtctctt ttttcatttc cgatctcggt
     3001 ccgggagaaa tacatcaggc tgtcgtggca ggcaaaagtc gccgggccgt catgataagt
     3061 ctgattgaac ttgcctgaga acgattttgg gtcggacatg tcctgaaaaa actgatccag
     3121 gtattttggc gatgagaaaa aaaggtcgag gtagttgaaa ttggtccatt catagcgctt
     3181 gtcatccata taattttgcc ggcgatcgga ggcgtaaaca atgccgtcgc catagaaagc
     3241 ggggccaaag tcggaacgga cggaattgag gttgttagca tttttaattt caaaagcggc
     3301 aggaatatcg tttaacttat caatctgggt gcagaaaccg gcatatgctt tgcctcgggg
     3361 gtcgtcggga accagcgccg catatttttc gtaggcttcc ctggccatgt cataatcctg
     3421 ggtgcaacgc agagcctgtc cgtaataaaa ccagttgata tcctgacttg agggcaggtt
     3481 tacagcgcgt gagtaccatg ctttggcatt aagctgatca ttggtaagcc ggtagcagtc
     3541 agcaagtctg ataatggcat ggtcccggtc cttgtgcccc ggtttttggg caattttaag
     3601 gtaatagggt atggccaggg cgtaactgta actcttgtat aatctgtcgg cttttttaac
     3661 ctgggcaaat gtcgtttcag cagccagcgt aagtattaac aaccatgcta aatattttct
     3721 gttcatatca ggggcattgc aaagatgagt gaaaaattac taaaagtacc ggggtgtcag
     3781 cattcggatt ctgagcaggt caaagtcgaa accaagccgt atttcgtgcg atcccgagtt
     3841 gcagttcctc agcgggctga agttataatc gtaagagtat ccgattcgta aactctttcc
     3901 gatcatgaac tctgtcattg caactaccgt gttgtcggta cgataggtag ctccgatcca
     3961 gaaagcgttg ttgatcagga aactggcgtt caggtccatc tgccaggggg cgtttatcac
     4021 gaattttatc aaagtggaag gcctgaatat gacctggtcg gagagtggta ttgcaattcc
     4081 ggccataccg taaaaatgcc tcaggagttt tgaataggct ttgccatcat tggtttcgac
     4141 cagggccatt tcattctgca acaactgctt tgaggatatg ccggcataca gatgatcgga
     4201 atagtagtaa acaccgaagt tggcatcagg aacaaacctg ttattctgac tgccgtaaaa
     4261 gtaatagtca ggatcttcga caaatacttt gctaaaatcg atatagttgt acttgaatcc
     4321 cgcggaaagc cccaacgaga gtttgccctt tggcaggatg atgcggtagg catatgtcgc
     4381 ctgaaatccc tggtcaatca tggcacctgt cttatcgtgg tatgcatagc atcccagtcc
     4441 catgtgatcg tttttcagcg gcgaatgcat gctcactgaa atggtattgg gcgctccgtc
     4501 gaatcctacc cactgataac ggctgattat atcggcgctg aagacttccc ggctgccggc
     4561 ataagccgga ttcaccgtaa gtttattgaa catatactgg ctaaacaggg catcctgttg
     4621 cgccatgcca ttcaaagccg taaataaaag gagtgttatg atcagttttc tcatcttagt
     4681 tgtgttttta gccatcgctt attcctcccc ccgcattaag atccatcctt tacgtgttcc
     4741 ggaattttca atctcaagta tatagaaata ggttccgtta ggcataagct ctccgttttc
     4801 gtttcggcca tcccacgagc gggttgtatt gttgtaatcg ccgatttttc taatcacgtc
     4861 accccatctg ttatagatgg taacagaatt ttcgggataa ttttcgattc cccttatttt
     4921 ccacttatca ttgatgccgt cgctgttggg cgaaagaccg ttgaaaatga acagatcgtt
     4981 gatgtccggt tgtttgacat gaataaatac aagcgtgtcg gaacaaattc cagactgatc
     5041 gtcggcgcaa atctcgtagc agaatgaatc atccccttca aaatccggat aaggagaata
     5101 tgtgattgta ttgtccgagt tgattattac gatgccatgc gacgggaagc tgcagaaact
     5161 tatcgtcagg ggcacgccgc ccgggttgat atcattagtt aatacggcga ccgttgtggg
     5221 cgtgttgacc aatgttgtat cgtagtcagc aacagctttg atgccgcctc cggaagcgct
     5281 gatgaagaca tccgtttgat cgcttgccat acaccctgtt aaaaggtcaa ggacagaaag
     5341 tgtgaatgta gtgtcggaat atagcggtaa tgtaacaggg tttgggacat tgggattcat
     5401 taccatttgg tcaggctgcc aattccatcc gtaaaatccg gaaccgcctg ttgccgaacc
     5461 tgccagaaca gttgaagcgc ctggctgaat tgtttgatcc ggccctgcat ttaccaccgg
     5521 ggctgaattt accgtgataa acatttcatc ggaaatgata acgcttccgc aggcaaggtt
     5581 tccctggaca ttgaggatta gtgtggcaat gcctgaaaat cctgatgccg gaacaaaggt
     5641 tggtgtcatg gaatcagcat ttatcagaat gccggcagat ggaggcgaaa cgctccacaa
     5701 tactgagctg taagaggcag caatagctcc tgaaaccgtg aaactctcat cctggcatac
     5761 agtagcatcc ggcccggcgc cggcaaccgg catctcaaca agagtaagga tcgcctgatc
     5821 ggaggaatcg gcacagcccg tgttacctgt tacattcaat gtaaggacga ccattcccag
     5881 gtttgcatca gcgacactag gagtatacac cggatttagt aacgagggat cgctgaaaga
     5941 tccggagccg tttgaactcc agtgcagcga aatataattc tgcgccagcg catcgataac
     6001 aggaacaggt gtaacattac aggtcgtcag atcgggccct gcatatgctg aggctttgga
     6061 atttacagcc agtatcatgg tatccgatac actgccgcat tccaggccgg tagcggtcag
     6121 gatgaggaaa atatcacccg tttctccggc agcaggtgta taggtgggcg taagggtagt
     6181 gaaatcaatc agagttccgg ctccgctggt agtccaaaac aggctgccgg ccatttgggc
     6241 ggaggcttcc gtcaccgtga aagaattccc ttcgcatata gtttcatccg gcccggccga
     6301 tgcggttgct gatttattaa gggtaagttt catgacgtcc gaccggtcgg ggcagggggc
     6361 atttccgcct gctgtcagcg aaaggaacac ataaccgttg tagatatcgg tcgggcttgg
     6421 ggtgtaaaca gggtcaagga tgttcggatt gtcgaaagtg cctgttccgg aagttgacca
     6481 tgtgagtgaa ctgtaattag cggcggtgct cccgatgata tggaattgtt cgccctggca
     6541 gactgcagta tcgggaccgg ctgataccaa cgcggttgaa ttgatttgaa gcgtcatcgt
     6601 atccgaaaca ttgttgcagg gataataggc aatgccattc aggataagca atatgattcc
     6661 ggtttctccg gaagccggtg tatagacagg cgtcagggta gtttctcctg tgagcgtacc
     6721 agtgccggtg gttgtccagt tgtaagcggc acagttttcg gcggttgcac cggtgacggt
     6781 gaacggccaa tcctggcagg cattgtcatc aggacctgca tctgccaccg gagatttcgt
     6841 gattgtgagt accatttggt ctgaattatc cgggcagggg gcaatcgcag ccaaatgtag
     6901 cgtcaggatc gcagatccgt ttataatatc cgtttcgctg ggagaataaa caggatgaag
     6961 gagggtcgga tcgttaaagg tgccggttcc cgatgtagtc cagagcaaac tactgtaatt
     7021 tgaggcaaat gaagaggtca ggatggcagt tgaaccttca cagaccggga tatcgttgcc
     7081 ggcataaacc gtcccgggca ggttgatctg aagttgcatc gtgtcagcag aattctcaca
     7141 aggataagct gctgttccgc tgaggatgag cagtattact ccggactctc ctgctgaggg
     7201 tgtataggta ggagtcaggg tcgaactgcc ggtcagcgtg ccggctccgg ttgttatcca
     7261 gttgtatgca atgcagtttt gggcggttgc tcctgtaacc gtaaatgctg aattctggca
     7321 taccggtgca tcgggtcccg catcaaccag tgcagattgg tttattgtaa ttattatttg
     7381 gtcggatgct tcgggacaag gttcatttgc gatagcatga agcgtaaggg ctacactgcc
     7441 attcagaata tctgtttcgc tgggggtgta ttcaggattg atgatggtgg ggtcactgaa
     7501 aatcccggta ccggaagagg tccacagcaa agagctgtaa tcactggctg aagcgcctga
     7561 caataatacc atggaacctt cacagatggt atcatccaac ccggcataag cggttgccgg
     7621 cgcgttaatg aaagcattca tcacatcagc cgaaacctga caattaccgt taacgatagt
     7681 ccattcaaaa gcatagatgc cgaaggtaag gcctgatact gctgtgttgg gatcggaagg
     7741 atcagctatg gtcgccgtat ttggcccgga tagttgcgtc caggttcccg ttccgaatac
     7801 cggaacattg ccggcaagca gggtagtcac atttcctgtt gcgtcgcaga attcctggtc
     7861 atttcctgca taagcagggg taggcgggtt aaagttgatg accatcattg tatcggtcga
     7921 aacgcacccg ttattatcaa tcgaatagat gaaatagtag ggcgtcgggc tggcaatcaa
     7981 acctgagact accgccacgc ttcccgtgga agggaaaacg gagggtgtgt tcgggccgga
     8041 aagaagcgac cagctcccga ttcccggatc cggtgtattt ccgataagaa aagtaacatc
     8101 agcattacac aaattctggt cgggtccggc aaaggctgtg attggttcaa tatccaggat
     8161 tgttactgaa gccgtgtcac tacatccgtt ggcgtcagtc acaatcacag tatatgtgcc
     8221 cgacggcagg tcaatggcgg tttgggtcgt ttgggtcgga gtggagttcc ataaataggt
     8281 atagtcgggt gtgcctcctg ttggtgtaac ggtggcataa cctgatgagc tgcctgtaca
     8341 actattaccc tgcgaagtta ttaaagcaat aagcaaaggc ggctcagtga cggggagagg
     8401 cggttggggt gcagcgggac atccgtttgc gtccgtcacg ctccagacgt atcctggccc
     8461 ggcgggaatg ccgttgaata tataatttcc aacttgcgtg acgccgttaa acgtacaggt
     8521 gtaaggagga acgccgttga tggggaatat tgaaatggta gcggttccgc cgtagcaaag
     8581 aatgggggat gttactgcgg caaaagcagt aggaacggga ggttccgtaa catcgagggg
     8641 tgtgccggcc acaggaccgc aaccattggc gtcggtgacg ctccaggtat atccgattcc
     8701 tgccgggatg cctgtaaaaa cgctgtctgc cgtctgtgtt gcaccgtcga aggtataggt
     8761 atagggaggc gtccccccgg aaacatgtat ggtaacggta gccgttccgc cgttacagat
     8821 gacgggtgcg gtgaccgaag cggtcgccgt aaccgggaga ggttccgtaa tatcgagagg
     8881 ggaggccggc gcgtcgcagc cattggcgtc ggtaacgctc cagaggtatc ctgttccggc
     8941 cggaatcccg gtaaaaacgc tatccccgga ttgggttatg ccattaaatg tataagtata
     9001 gggaggtgtt ccgcctgaaa cactaatcgt gacggtagcc gtttcaccgt tacaaaggat
     9061 aggggaggtt acagaggcag aagcgacagg tgcgtcaggc gaagtcacgt cgatagtgtc
     9121 gttcacgggg ccgcagtcgt tgtcatccgt cacacttgcg atatatcctg ttccggccgg
     9181 gataccggta aacacgccgt tgccggtctg ggtaacgccg ttaaaggtat aggtataagg
     9241 cggggttccc ccgttggcca caattgtaac cgtagcagtt cctccggaac atgttggggg
     9301 aacggtaact gaagccgtca cggtaatctg gggtggctca gtgacgggga gaggcggttg
     9361 gggtgcagcg ggacatccgt ttgcgtccgt cacgctccag acgtatcctg gcccggcggg
     9421 aatgccgttg aatatataat ttccaacttg cgtgacgccg ttaaacgtac aggtgtaagg
     9481 aggaacgccg ttgatgggga atattgaaat ggtagcggtt ccgccgtagc aaagaatggg
     9541 ggatgttact gcggcaaaag cagtaggaac gggaggttcc gtaacatcga ggggtgtgcc
     9601 ggccacagga ccgcaaccat tggcgtcggt gacgctccag gtatatccga ttcctgccgg
     9661 gatgcctgta aaaacgctgt ctgccgtctg tgttgcaccg tcgaaggtat aggtataggg
     9721 aggcgtcccc ccggaaacat gtatggtaac ggtagccgtt ccgccgttac agatgacggg
     9781 tgcggtgacc gaagcggtcg ccgtaaccgg gagaggttcc gtaatatcga gaggggaggc
     9841 cggcgcgtcg cagccattgg cgtcggtaac gctccagagg tatcctgttc cggccggaat
     9901 cccggtaaaa acgctatccc cggattgggt tatgccatta aatgtataag tatagggagg
     9961 tgttccgcct gaaacgctaa tcgtgacggt agccgtttca ccagcgcaac tgattggcgc
    10021 agttattatt gctgttgctg aaagaatgtc ggattcaatg atatcaaggg tgtcggttac
    10081 agtgctgcag gcgtttgcat ccgtaacact ccagatgtaa cctgtgcctg caggaatacc
    10141 ggtaaagaca ttattgccta cctgaatgac accgttaaaa gtataggtat aaggcaaagt
    10201 tcccccgctt gccataattg tgactgttgc agtactccct gagcaggaaa caggaggcgt
    10261 gggaacaaag gcttcgaggg tatcgggttc cattacggag agcagaccgg aaatagtatc
    10321 gcaatggttg gtgtcaaata cgttccacaa gtaaccggcg cttgccggaa tgcctgtgaa
    10381 tacaccattt ccaacctggg ttatcccgtt aaaggtatat gtatagggag ccgttccccc
    10441 tgcagcattt atcgtaacgg ttgcagtacc cccgggacac agtatagggg taaaaacgac
    10501 atccgcaatt agcggtgaag gttctgtaat ggtgacagtt tgtgtttcag ggccacagtt
    10561 gttatcatcg tcaacgctga cggtgtacgt tcctgaagat agtccgctgg ctgttgcggt
    10621 atattgaggg ggcaccgtgt tccaaacata agtgtaagga ggtgttccac cgccggctaa
    10681 aacagtagcg cttcctgatg cttgtccgaa gcagaggttg ttggtctggc cggttaaagc
    10741 aacagtcaga ggtaaagcgg gttgggttat caggaagggc agcgcggtat cgcagccatt
    10801 gttatcggtt atatataagg tatacgatcc ggcagcaagg cctgaaaatg tgtttccagc
    10861 ctggaaaatt atattgtcaa tactaaaccg gtaaggggga gtgcctccgg aagcaacggc
    10921 tgttaccgag ccggtttcat cgccataaca taacacattg gaaatatctg taattgatgc
    10981 gatcagggct gatgccggtt gtgttatcgt aatgctgtcg atggcataac agccattcgt
    11041 gtcgtaaacg gttaccgtat agttgccggc tgtaagattt atggcagtag ccgtgttttg
    11101 cggtgggaca gtattccagg aataggtata aggggcagcg ccattggtta cggttactgt
    11161 tgccgttcca tcgttccttc catagcaggt aacaggtgtg ccggccatgt tcagtatcat
    11221 gggaaggtcc tgaacaacgc ttacaatttg tgcacattga cttgaaaggc cgtggatatc
    11281 agtcacagtc cagatgacag gagtatcgcc gagaggaaat tgcaccgggg cgtcgttgat
    11341 gaggctgaga gtgtcgcaat tatcatcaac aatgggttcg gtcagtatca caccacctgc
    11401 aaatgtttcg caactgggta ttgtaatgat ggtatcagaa ggacatatta ttgatggata
    11461 ttcgctgtct tcaatattca ccgtgaaact gcatgtagcc gacaatccat cggcatctgt
    11521 cacggtatag gttacggctg tagcgccata gttgaaaagg taagggttca gaatattatt
    11581 aatgcctgtt gaagaggagg tgtctacagt tgcaccggcc attatccagg taagagatat
    11641 tatattgtta tcgggatcat tataagttgc tgcaatgttg ttgatgattg ccccgcaaat
    11701 cccaacatct gatcctgaat tgacgacagg ccccggacag gttatggcag gagcataatt
    11761 aactgcgtta atatggattc taacaatagc ggtatcacaa ctgccgtatg aatcgcaaat
    11821 ctggtaaaca aaattgtcat caccgtttac attggggttg ggcgtatacg tgaccgtatt
    11881 gtctgcattg aggatagcag ttccgagtga aggcaattca acaaaagaaa ccgggtttac
    11941 tgttgcaggc ccatggcatg gaaaataatc attattcaat atcgggagcg taactgatgt
    12001 attctccggg atggtcgctg agttaggcct ggccagtggt ccgtcgacgc cggtcaactg
    12061 gaaattatcg ataaacagcc ttccgtcgcc ggctcctcct gtgccattgg atgcgaatag
    12121 ccggatatac aggttcgacg ggttattaat caggcttacc gacgaccagt caagtacttt
    12181 ctcataccag gtattgctca atagcagcga catggaacca ttgatcgtgt aacttgtagg
    12241 gtcggtgcta taggaaaagt tgatggcctg ggcggcacgg tttcccctcc gggcctgaac
    12301 atagagcttg aatctgcggt acttgtaaag gctgtcgccg cctacatgga actggaaata
    12361 ccgcgtattg gtgccggttg agggatccat gaaaatggcg gggtttgctg cagaatcgct
    12421 cacaaatgcc aggctccctg ctgcagtgcc cgtcgtaccg agaatattaa acggctcgct
    12481 tgaggtagct tcgcaagtga tgccaccggc agtttgggtc gggctggctg gatatgctgc
    12541 agccccgaca aaattgtacc gggtcagaag agtggcttcg gtcacgtaca ccgattgtgt
    12601 gcaggtggcg gtgagcccgt taacatcagt ggcaacccag gtgattgtgg taagacctgc
    12661 cgggtaagcg tcgctgagca gaagaccatc gctgcgcgta ccaacaactg aagcaatacc
    12721 gcacgaatca acagcaacgg gagttccaat attaaccatt gcatcacaat tgccttcatc
    12781 ataggtcgca cggatattaa gcgggcatgt gatgacaggc ggttggttat cgttgactgt
    12841 tacggtgaaa ctgcaggagt cggataatcc cccgtcgtcg gttaacgtgt atgttaccgt
    12901 gtttattcct gcgtgaaaaa cgtaggaagt caggttattt atccccgtca aaggcgatga
    12961 gccagtagtg gctccggtta ttgtccaggt gagtgtagtg atatctccgt caggatcgtt
    13021 ataaatggct gaaaggccgg ctgtgacatt ggcggtgcaa aatccagggt cggttacggt
    13081 agtcaggttt cccgggcagg tgatgacagg ctgtgcaggg attgcaggca ggtaatattt
    13141 ttcagggatc agcaacgaat ggctggagga attaatatca ggagatgatg aacagtctgg
    13201 gctggaaatt ggataggcat cgggcgcttg ctgtaaatgt gcagtaattc gcttatcaga
    13261 taaaccggcg gcattttcag atgcgtggaa tacagaatac ctgtcaggtc cggacgcatg
    13321 agcccccggg ttcggaaata agataaaagg catgagcaac aacagtgtta cacatgcctt
    13381 ttcaacgagt gaaagaaata taggtttttt catattactg tccctgagtc attaatgctt
    13441 taagtcacaa cccaaaatca agaatagcct gcaacaaaag gcaattgagt actgaatagt
    13501 tttacctgtg cctgttattt gtacttttca cggatagctg tttttccaaa cgggactaat
    13561 ccattgttaa atttaaagct taaaaaagcg tgcttttgtt aggttgaaat aagttattaa
    13621 atgatttatt aacaataaaa atggattaaa gtacagtaga tcaatagtat agacctaaaa
    13681 aagccattaa ttccgggttt tgttgaaaaa gatttgattg ctgtttgagg aaaattacag
    13741 tatgttaatt ctcaatttgc acgatattaa aattcccctc tgacagaaat atccggtttt
    13801 atccgagaca atcagccctc attacaggca acagtatttt taaaagcatt aaataatcgt
    13861 aggtttgtac cgttatcatc aaaaaaggga tatgaaattt aagaacagca acattctggt
    13921 aatcgtactg ctgatatttt gtttaggagc cggatataat catacctgtg cccaggatga
    13981 ttatcagggg agcctcaaag gcgccccgct aaaggaacgg ctgttctatg ggggaggact
    14041 tggcttgcag gtcggcagca ttacacttat cgatgtttcg cccatgattg gttataagct
    14101 gtccaaccgc attggaatcg gggtaagtcc cacatataaa tactatcact ggcaagatta
    14161 ttttgggaaa gacctggatt taaaaaccaa tgtcttcggg gggagcgtgt ttgcacgatg
    14221 ctatgtttac cagggattgt ttgcccaaag tgaatatgag taccttaggt ataaatatga
    14281 caatcctgca tcttcgggaa aattcaccaa tgatatatgg agcttgttta taggcggagg
    14341 ttacagtcaa ccgttaggcg gcagggcgtc catgtatatt atggcattat ggaacctgca
    14401 cgatacgcag gactcccctt ataacaaccc cctgatcagg atcgggatga gtttgggatt
    14461 ttaaagtacc gctattacaa aaaatacccg ggaaaatttt aatcccgggt atagagcaaa
    14521 atttattaaa agaacaggct taagctgttt tttcaggaat gtttctgagt atttcgattg
    14581 tcaggtccca gaatcgcaga accgtggcta tcagcagacg ttcatcagga gaatgggccc
    14641 ctttgatggt tgggccgtag gaaatcatat caagcccggt atatttttcg ccgaaaagtc
    14701 cgcactcaag tccggcatga attgcgagga ctttcggatc ggaatggaag aggtcggtat
    14761 aggctttcct tgtgatttcc agaattcttg aatcggggtt tggcgtccag cctgggtatc
    14821 cgtcgccatg ggtcactttg gcccctgcca ggcggaacac gctggcaacc atactcacca
    14881 catcttcttt gcctgactcc gaagaacttc gctggctggt ggtaatctct atatttttat
    14941 cgttgatttt aaccgacgcc aggtttgtgg aagtatcaac gaaattgggg atatcggccg
    15001 acatggttat tactccgtgc gggcaggcat ataaggcatt gagcagacgc tcctgggatg
    15061 acaggtcgat tacataggac ggcagttcgg ccggggcata actaaccagt aattcaggtt
    15121 cggttgtttt tagctccttt ttcaccatgt tgttaaaatc cctgacgtaa ccggcgaaat
    15181 cgttttggaa tcgttttggc agcgtgatga gcgcaaaagc ctcacgggcg atagcattac
    15241 gaaggttccc gccctttagg tccgaaattc gcgtttcata tttctgggct gtgttccaca
    15301 acaggcggtt gatgattttg ttcgcattgc caaggccttt gttgatatcg tcgcccgaat
    15361 ggccgccttt caggcctgat acacggatcg taaaggcgac actgtcgtaa ttgacaggaa
    15421 ccttggaata gttcatgctg ataatcgtat ccttgcctcc ggcgcagccg ataaagagtt
    15481 ctccttcgtc ttccgagtca aggttcagga gaattttact gtcgaaaaat ccgggcttta
    15541 aaccgtaagc tcctgtaagg cctgtttctt catcaacggt aaagaggaat tcaagcgggc
    15601 cgtgtttaac ggttttatct tccattaagg ccagttgtac ggcaatacca atgccgtcgt
    15661 cggcgccgag ggtcgttccg cgggctttta cccattcccc atcaataaaa ggttgtatgg
    15721 catcgtggtc aaaatcgtga taagtatcac tgttcttttc acaaaccata tccacatggc
    15781 attggagcac gacgctgcat ctgttttcat atccgggcgt tgcaggtttt tttacaatta
    15841 cattcccggt ttcatccatg taattctcaa gcgacaatgc cccggcaacc tgccggatat
    15901 attgtgttat ctgggtttcc tttttagaag ggcgggggac tttgcaagtt tcggcaaaat
    15961 agtgccaaac tctttctggt tgcaaacctt taaaaacatc attcattttc aaataaactt
    16021 atggacagat gacaaatgta taagatttta acggtttcga aacaatgtat aaaaggaatt
    16081 ttatctgcga accattttta catttttacg attcgcttca gaaaggggaa ttctctctat
    16141 tctattgagt tgccgttttt ttcttgtagt ttatatgctg tgcttcaacc gatggattgt
    16201 atccttcggg tcggcagatg agaaaaccgt attgccggcg accaggacat ccacgccggc
    16261 ttctgccagt ttctgcgcat ttgacagcac aacgccgcca tcaacctcaa taaggacatc
    16321 aggattcaca tccaggatca tctttttcag ttcccggatt ttccggtacg agttttcgat
    16381 gaacttttgc ccgccgaagc cgggattaac cgtcatcagc aacaccatgt cgacatcagg
    16441 gagaatatcg gtgaggacat tcaccggtgt atgaggattg agaactacgc ctgctttcat
    16501 gcccagttct ttgatctgtt gaatggaacg gtgaaggtgg atgcaggctt cataatgaac
    16561 agtcaggatg tcggcgccgg catcgcggaa cgccgtgaaa tgcctgtcgg gctcaacgat
    16621 catcaggtga acatccagag gcttttgtga tatctttttt accgctttta ttaccggcat
    16681 tccaaaagtg atgttaggga caaagactcc atccatcaca tcaacatgga cccagtcggc
    16741 ttcactgttg ttgatcattt ctacggctga agccaggttt gtaaagtcgg cggttagtat
    16801 tgaaggggag ataaggggca tgggattgaa gttaaaagtc aaaagttaaa agtcaaaagt
    16861 caaagtttgc ctgccttgcg taagctggcg ggtggatggc agggtcaaag tttaccttgc
    16921 gaagggctgt gggtgagacg gcagggtgaa aagttaagag taaagagttc agagttttaa
    16981 agtatgcttg ccttaacgaa cgctggttcg gggcaggcag gtttgagagt ttaaagttag
    17041 ggaaattacc tgaaagcaaa aaaaaagagc ggctataccg ccgctccaaa aacttatcca
    17101 aggtaagttt ttaaaatctt actcctagag gtgtgtttca gcctgcggat ggctttctcc
    17161 tttatctggc ggacacgctc gcgggtgagg tcgaattttt caccgatttc ttccagcgtg
    17221 agcggatggc tgccgttaag cccgaagtaa tattttatca cttccgattc gcgggttgtc
    17281 agggttgaga tagcacgatc gatttccttg cggagcgatt catacaggag tccggtttca
    17341 ggggtagggc cgtcgtcgct gcgcagcaca tcgtacatgg tgttatcctc atcctgaacc
    17401 agcggggcat ccatcgaaac atgccggcct gaattacgca tcgactcttt tacctcgttt
    17461 tcgctgattt cgagcatcgt ggcaatttca tcgctattgg gctcgcgttc aaactcctgt
    17521 tcaagtttgg cgtaagcttt attaattttg ttgatagccc cgattttgtt cagcggcagc
    17581 cttacaatac gcgattgttc ggccaatgcc tggaggatgg attgacggat ccaccatacg
    17641 gcataagaaa tgaatttaaa tccgcgtgtt tcgtcaaaac gttgtgcggc tttgattaag
    17701 cccaggttgc cttcgttgat gaggtcgggg aggcttaatc cctggttctg gtattgtttt
    17761 gaaaccgaaa ccacgaacct gagatttgct ttggtgagtt tttcgagcgc tgcacggtcg
    17821 ccttgtttta tccgctgggc aagcatcact tcttcttcag ccgtgatgag ttccacttta
    17881 cctatttcct ggagatactt gtcgagcgaa gctgtttcgc ggtttgtaac ctgtttagta
    17941 atttttaatt gcctcataga cagaattcag tgttgtgaaa accttttacg aagaaacctt
    18001 tgtccaggtt tcaaatggtt gaaaaaaaat tgccttccgg gaattgactc gccggaaggc
    18061 attgaaaatt aagcctggtt gtttttctct tcgccttcgg gttttggcaa taacactttt
    18121 ctcgaaagtt tgagttttcc tgttttactg tctatatcaa tgagcttaac atctatctca
    18181 tcgcctactt tcaggacgct ttcaacgtct ttcaggcggc gccattcgat ttccgaaatg
    18241 tgaagaagcc cgtcttttcc cggaaggatt tcaacgaaag cgccaaaagt tgtgatggtt
    18301 ttgacggttc cgtggtaaac ttcaccgatt tcgggaacag cgacaatttt cttgattttg
    18361 gctttaacgg cttcaatgga ttcaaggttg ctcgaaacga tgtcgatcac gcccagttca
    18421 cctacttctt caatcacaat ggtggagttg gtttcgcgct gcatttcctg gatgatcttt
    18481 ccgcctgggc ctattacggc gccaataaat tcgcgcggaa tggtcatttt tacgatacgc
    18541 gggacatgcg gcttgtaatc ttcacgtggt ttggtaatgg tttttgccat ctcaccaagg
    18601 atgtgaagcc ttccttttct tgcctgttca agggcttcgg ccaggatgtc gtaacgcagt
    18661 ccgtcaattt tgatatccat ctggcaggct gtgatgccgt cggctgttcc ggttactttg
    18721 aaatccatat ctcccaggtg gtcttcatcg ccgaggatgt cgctgagtac ggcgtatttt
    18781 tcaccatcgg tgataagccc catggcgatg ccggcgaccg gttttacgag ttttacaccg
    18841 gcgtcgagga gcgccagcgt tccggcacaa accgtagcca tcgaagagga accattcgat
    18901 tcgagaatat cggaaaccac gcgaacagta tacgggttgg tttctgcatc gggcatcatg
    18961 gctttgagcg cgcgcagcgc caggtttccg tggcctattt cgcgacggcc aacgccgcgg
    19021 atgggtttta tttctcccgt agcaaaaggc gggaaattat aatggaggat gaaattctgc
    19081 ttttcttcga ttacggcgcc gtcgatgacc tgttcatcga gtttggtgcc aagggtaacg
    19141 gtagtaagcg actgggtttc accgcgggta aaaatggctg aaccgtgggc tgcaggaagg
    19201 taatcaactt cactccaaat agggcggatt tgatcgagtt tgcggccatc aagtcgtttc
    19261 cggtcgttga ggatcatatc ccgcatggct tttttctcca cgtcgtgata ataacgatta
    19321 accagaaatg ctttttccga agcttcttcc tcggtaagtg tttcgataaa ttctttcttc
    19381 accgattcaa aaccttcggt gcgctctttt ttacctaatc cggaggaagc aacggcgtag
    19441 attttatcat aacagaattt ggtcagggcc tggcgcagtt cctcgtcatg ggtttcgtgg
    19501 cagtattcct gtttcgggct tgatttttca accatactgc ccagttctaa ttgtgctttg
    19561 caggcaagtt taatatgctc gtgagcggtt ttcagcgcct ggagcatggc ttcttcgctt
    19621 acttctttca tttcgccttc caccatcata atgttgtcca tggttgcggc aacgatgata
    19681 tcgatttcgg cagcaggaag atcggcagcg ggcgggttga tgatgtattt atcattaatc
    19741 cgggctacgc gcacttcgct taccggtccg ttgaacggaa tgtcggagac tgccagggcg
    19801 gttgaagcag ccaggcaggc cagcgaatcg ggtaatatat tggtgtcggc cgaaatcagg
    19861 gtgataataa cctgtacctc agcgtgataa tcgtccggga ataagggacg taatgccctg
    19921 tcgaccaacc tggagatcag gatttcatga tccgaaggac gggcttcgcg tttgaagaat
    19981 ccgccgggaa atcggcccga agcggcatat ttttccctgt attcaacact caggggcatg
    20041 aagtcaacat cttccttggc ctctttggca gcaacaactg tggccagcaa catggtgtta
    20101 cccatcttga ccacgcatga accgtcggct tgttttgcaa gtttcccggt ttcaagtgaa
    20161 caaaggcgac catcgccaat gtcgaaagtt tttacaattc cttccataat ttttttttac
    20221 ctcttttatt aacgtgtgcg ccgttgacat ctgggagagg aggctgaaaa cggcgccctg
    20281 attcagcttc ctcgcttttt tgtattgcac tgttatgggt tgtttataaa cccttaacag
    20341 gattgcttac gattctgttt tatatttctt attactgtca ataatacaaa aaaaggcaat
    20401 ttgaaaattg ccttttcctg atagatatat tttttatttc cggtattatt tacgaagact
    20461 gagcttggcg ataatagcac ggtagcgttc aatgtctttt tccttaaggt aatcgagcat
    20521 acggcggcgt ttaccaacca aacgcatcaa agagcgttgt gtggcaaggt ctttgcggtt
    20581 cgatttcaga tgctctgtaa gatgcttgat ccggtaagtg aaaagtgcta tctggccttc
    20641 ggctgaaccg gtatcggcat cggatttacc attgtccttg aaaaggtctt tcttgatttc
    20701 tggcgttaaa tacatatctt tctggcgttt ataacctgtt tttcagaatc gggctgcaaa
    20761 attactattt tttatcagtt gtacaaagtt ttctgatttt ttttaataaa aagtatttac
    20821 ggagccgctc tctggctgca aaggtaacaa tttcatcgaa atggatttgc cggttcactg
    20881 aacaaattat tcggaccgaa aataaaaggg ttggcagcct tcagcgatta gccgggcaag
    20941 cgctggttta agacgaaacc acaataaatg cgtattttta ccgacgtttg tttttctttt
    21001 ttttttactg gcgacatgaa aacagacctg gctgatatga tgacaaccac ggaaaatcag
    21061 gttatattgg tggacggctc tgataatgcc cttgctgcaa tgccgaaatt gaaggctcat
    21121 gaggaagggg cgctgcacag ggctgtttcg gtaatcatca tgaacagccg gaacgagatg
    21181 cttctccagc agcgggctta taccaagtac cactcttcag gtttatggtc caatacggca
    21241 tgcagccatc cctgtcccgg tgaatcggcg catcgggctg caacccgcag gctgcaggag
    21301 gagatgggta taacatgccg gttaaagaaa gtttttgatt ttacttaccg ggcgcagttt
    21361 gcgaatgggc tgacggagca tgaattcgac catgttttct ttggaagata tgacggagat
    21421 cccatgatca atcctgttga agttcatgat tttaaatgga tgcctgttca ggatatcctg
    21481 aatggtctgc gggcaaaccc tcagacgtat accgtgtggt tccgtattat gatggagcaa
    21541 atagcgaacc ggccggaatt gttttcaggc cggtaaccgg acggctatga acgggcagaa
    21601 aatccctgcc gcagatcgtt gcccgatggg ctctgaaaaa catctaatcc gaattccggt
    21661 acgattgcca tgatatgatc gaaaatgtcg gattggacat tttcataact caaccattgc
    21721 gtttcggtgg tgaaggcata gatttcaaca gggattcctg aagaagtagg ttgcaattgc
    21781 ctcacgagca ggtcaaaatc tttatgtacc tgggggtgcg catgcagata ggcacccaga
    21841 taagccctga aaacgccgat gttcgtttgg cggcgtccgt ttaccggaac cgaaccgtcg
    21901 atattgtttt cagcattgta ttgaaggagc tcagactgtt tcttgtcaat gtagtctttc
    21961 agaatgtcaa tttttctgaa cctttccagc atttcggggg tgcagaattt cacgcttgcc
    22021 atatcgatgt tgatggaacg tttcattcgt ctggctcccg attccgtcat gccccggtag
    22081 ttctgaaacg attccgatac gagggaataa gtgggaagtg tggtaatggt gttatcccag
    22141 ttctgaactt ttacggtagt gagtgagatc tcaagtacag ttccgtcggc gccgaatttt
    22201 ggcatattga tccagtcgcc ttgccttacc atatcgttgg ccgaaagctg tatccccccg
    22261 acaaatccca ggatgggatc tttaaaaacc agtaacagta cggctgaaaa agcgccaagc
    22321 cccgtcaaca taacccaggg cgactggttg aaaaagtagg agagtatcag gagcgctcca
    22381 attgtgaaga caatgatctt tccaacctgg ataaatccct ttaccggatg atatctggca
    22441 taatcggtat cctggcagat ggcatagacg gcgttgaaaa acgaattgat caccagcaga
    22501 aataccatga tgatataaac gttggtgaac ttgatggcca tgctgatggt ggaatgggaa
    22561 aattcactca gcgtggatgg tatcgactgg tagactatga tgccgggtat gaggaaagcc
    22621 atccggttga atactttatt ttcgagaagg gcatcatccc acatgctttt tgtccttcgg
    22681 gcaatacgcc tgactaccct tatgataatg aatttagcca ggtagtaaag caggcaggca
    22741 atggcaagga cgatgatgaa atcgttgacg cccgctatct tcatggctgt tgcctcctgc
    22801 attcctgcgc gggttagcca tttggtgatt atttcggtga gatgctggaa catacggtgc
    22861 ttaggaattg aggttatgct gcaaagttaa tgacctgtaa gtttataagt aaatcactat
    22921 ggtttgagtt cagcatttat cgggcggttg gaagtaagaa aggctataat ccgccggttt
    22981 ccggcttccg gtctattttc agtttccttt gtcccgacac atttttgatt tcacctccag
    23041 cttataccaa agggaaatga ccgggagaac ctccggtaac gcctgataaa tcaaccgcca
    23101 gcctgcttta ctttgtagcc tgcagcggta aggatttcga gaatttttgc ccgaaaatca
    23161 ccctgaatca aaatttcacc gtctttcacc gcgcctccgg tcccgcattt ggttttcagg
    23221 agttttccca atgtttcaag atcttcaggt tgtccgataa aaccggttat cagggtgacc
    23281 tgttttcccc cgcgctgttt gcggtcgagg gttacccgca ggttttgcat ccggggtggc
    23341 agggtttctg tttccgggat ttcattgtaa cggtattcga aatccggatt agtggaataa
    23401 acgaccccgg ttggtctgct ttttttaggc ataatttgaa ggatctttta tggattcttc
    23461 ctgaacctgt tcgcgttaag gaacgatcac gttgagcgtc aaaggtttaa cttccagttt
    23521 gaggtcgttg tcaatccggg ccggctctcc gtcaatattt accacactgc ccgctgtacc
    23581 gggaatttca accgttctgg cacgaaaata ttcggcatag ttggtttttc ctagcgttcc
    23641 gaagaaaatg cggggggcgg caaagaagat ggccttgatc aacgatggct tgcggattat
    23701 gcagatgtcc agccagccgt cgcaactgct ggcctgcggt gcaatacgcg catgaaaacc
    23761 aaactggttg ctgttggcaa atgaaatcat aagggcttcg cgttcaatgg attgtccgtc
    23821 gattagcagc cgatatttat tacaggtata acgtctgtaa tctttaagaa taatagcagt
    23881 ataggataac aatccacgga catctgattt gtcgaatttt tcggctacaa cggcgtcgaa
    23941 gccgattccg gcaatactgg taaaaatccg gtcgtttaat tgcacggtat cgatcaggcg
    24001 ggtgtttccc ctggctatca cccgcatggc atgaatgagg ttgagcggga tgccaaggtg
    24061 cctcgccaga ccgtttcccg acccgaaagg aaggattccc atgggaactc cggttccgaa
    24121 aagcgccgtt gctacttcgt tgatggtgcc gtcgcctccg cctgcgacta tgatatcata
    24181 atggtcttcg atggcttttt ctgacagttc ggtagcatgg tgggggtatt ttgtaaacac
    24241 aattgaggtt tcattgccat taccgaaaag caaacttaca tgcagcctca gcaatgcttc
    24301 gattttcgga gtaattcccg ccttgggatt gataataaac agtacttttt tcttttctcc
    24361 tgccataacc tattttatta tcaagctgtt actgatcatc cggttgttat actgctggat
    24421 atatgatttc agcaggttct ccattgctgt cctttgcttc agattgacat tcgcaaggtt
    24481 tttcatgagg aggctatcgg atttaaaatc atacaactgg gtttgtgata tgccatccca
    24541 ttccagcgac caaccatctt tgataatctg gtatgatccg ttgatacttg aaattcccgc
    24601 atgagatgca gtgacatcaa agacggaatt gccgaaggca ataaacggtt ttgtgtaacc
    24661 caaatattga agtattgacg gcatgatgtc cgtttgctgg gctgtaatat tgctgttcgt
    24721 gttcagaacg gaatcctgct gatagaaaat aatcggaatg gaatactgcc ctgcacggct
    24781 ctggtaaaaa ggttcagccg attcagaggt atggtcggcg gtgatgacaa acaacgtatt
    24841 tgagaaccag ggcatttttt ttgccgtttc gaaaaaacgt ttcagggcat aatcggaata
    24901 catgaccgat tgctgaatag gcaattttcc tttccggaat tttcctttgt atttttcggg
    24961 aattttataa ggatggtgcg atgataatgt gaaaaccgac gcgagaaaag gttgtggcat
    25021 cgcgttgagt ttacctgcca taaactgcag gaacggctca tcccagatgc cccatttacc
    25081 gtcatagtcc ccgtcgttgc catattcatt cctgccgaaa taagcgtcga agcctgctga
    25141 acggcaaaaa gcatcaaatc ccatcgtgcc gttgttgccg ccatggaaaa atgcagaata
    25201 ataaccttcg ggtttcagca aaccggccag gctgttgaat gtattacctg cataggaaga
    25261 tgtaatgaaa tcctccgtca tccaggtagg aaggcttgcc aggatagccg gtattccctc
    25321 gatggaccgt ttgccgtttg cgtacccgtc caatacaagg ctgtgctcca tcaaactgtc
    25381 tagaaaagga gtaaacccca gataacgccc gttttccagt ttagggttaa ggctgccgga
    25441 atgttccttc gagaaacttt cgagaatgat aataaccaca ttccggcggt ccatgggcct
    25501 gtttgctccg gtactgtcag atcgggggta ttgcttggaa gcattgaaaa agccaagcat
    25561 ctgtttttcg tcactgaaat attccttttt ctggaggccg ttctgctcca gggtccggag
    25621 aatggtaaag gcagaattca ggaccagcgg cgtttgctgc acaggggcat agctgccggc
    25681 agtcataatt ccgatgggct ttagctggac accgcctctc atgccgagta ccgttaaccc
    25741 ggccatgatg actgtgatca gggtttggcc tgtgatatac caaccttttc cctgaacagg
    25801 tttgcggctg aatactacga tccggctgca aatcagcacc atcagcacaa caaatacaat
    25861 ccagagtaat aatacatacc agaaatcgtg aagaaattgc ggcatcatgt gggcggtatc
    25921 atttccaagc gtaatatacc ggaatatatc ggccgtaaga cgcttcatgg tgaacgggaa
    25981 atagacggta tcgacgaagt taggaatgaa cccgatagca tttgtaacat agaatatgct
    26041 gccggctatc cgctgatatg accggttcat gcgaaacctg aagggcagaa gcatgagcag
    26101 gatatagggc aggttgatgt atagcaatgc gcttgcatcg aaccgcaatc cagttagggc
    26161 ataataggca accctggaag ccggaacgtc ggggaacaat ggcttgttca ggaaaaaaag
    26221 cagcatcctc gaagccatca tcagcaacag gatcgcgaac aaacgactga aaagtacaat
    26281 ttgaggatta ccttgaatga ggggtttcgg ggtgttcgtt ttcattattg atgggaaaag
    26341 tccgggatca gggatgatga gaaatgaatc agtacaaaga gtagttgctt tccgggttat
    26401 ttgaaatatt taacaatgac ttcacggtta acttcattgg ctttgttgcc gtcgctgtcg
    26461 gttacattgt aataaacata acaggttccg ggtttcgtgg tatcaacctg gctatcgacg
    26521 attatttttg aggtaatatc gccgtcttct tcatcgtaag ctgaggcgcc ggcatcatga
    26581 taggacagcc ctacgccggt tgtcgctgga tttttaccaa ggatggtgat aaccggcggc
    26641 gcgccgtcct tcttgcaacc gctgaatgct gccagaaaca ggatgaaaaa tattaattta
    26701 atggttttca taagaatgcc ggccttttca ttacggcaaa aataaaaaaa taagagatgc
    26761 cccggggaca tctcttattc ataattatta atcaggcgag gttttattat ttttggactt
    26821 ttacattgta agtaatgctg ccctggctgc ctgtctgaat atcggtaacg cggataagcc
    26881 ctaatttgcc ggaagcggtt ttgaaagcta caattttgcc aacggccagt tcccttgcat
    26941 aggtatcgtt tgttgcactg gcataagcta tgatcatggc atcatcggtg atgttatccc
    27001 aaaggatctg gtcggggaca atgaagaatg tagtggcatt cctgacagac cactgtgaca
    27061 gcccataggt ggggtttgtg tatacattgt tcatggcagt ggggtcattc ggcgcagcca
    27121 gtgtagcacc atgatcggaa atgatctccg atccatagta atagcaccag tcaattttag
    27181 cctggttgtt gtaagcatca acgagtttat atacagtgcc gtcggccgat ccgaagaagc
    27241 tgccataggt gttgttataa taagagccga gaatcttctg atcaaaggtg ttgatttcac
    27301 cgccggcagt cgtggttatg atgaaagaaa tctcagcata ttcgccgttg gcgtcggtaa
    27361 ctttgaagct ccatgtttcc gaaccggcaa taaaagctgc attggcagta atttcgcctg
    27421 tgaacgagga ttggtcgatt gtttcgtcgg ttacaacgac ttcatttccg cccagggtgc
    27481 gaacaacttt caggttggtg agcttggcgc ccgaactgga attggctgca gccgaaatat
    27541 ttacggtaaa tccttcaccg gctttcaaag tggcgtcgcc tgaaattaac ccggaaccgg
    27601 taacaaaact aagggttggt gaaagatcct ctttttcgtc aggagtacag gcggagaaaa
    27661 aggaaacacc ggccatcacg aggaatagga ccgcaattaa actgattttt ttcataggta
    27721 agttgttatt ggtgaattat tttgttatca ggatgaaatc cgcagcgaaa ttactaaata
    27781 ttcggcctga tttcaaattt gcttttgtgt ttatcaaatt gcatgccaaa gaaagcgaat
    27841 tattgggcga ttggtcaaaa atgtggatga aatgaccgtt agatattaac ggcctgattg
    27901 ttaataaaaa aattgattgt taatggctcc gaaataaaaa ttaatttgct tttgcttttt
    27961 taatttttat atttttgcgg caaaattaac caactaaaat aaacaacaaa tgaaaaaact
    28021 gttcctcttc ttcgctgttg ccggcctgat ctcattcgca gcttgtaaat caagcgaaaa
    28081 caaaaccgaa gcaactgctg acacaactgc tcaagttgaa actaccgttg atactactgc
    28141 tgctgctcct gtagctgaca ccactgtagc caaataacac aggttcaact gttcggttaa
    28201 ttaattataa agaaaggaag cgatccgctt cctttttttt atgtccggta acgaaacgga
    28261 gatcaaaatt caagtttcgg cattccagcc tgaaatgaca gctatcataa aggaaatcag
    28321 ttgtttgaag gtgattttca ggggcgggaa aggctgaaaa ttaaatcctt ggttaacttt
    28381 gtttagaata ttgaagccag tacctttgcg ggataatgag aagtatagag caaaacaggt
    28441 actcaaaaat aaatatgacc cctatggatt ttaacgctga aaaatcggtt actgtcggta
    28501 ataaaggtaa aggtatccgt tccgattgcc atattaccct ggaacttacc aatgaaggag
    28561 gcaggcagat tgccatcgag agtaaagtac aggtgttgta tggcgatgat atccgccggc
    28621 aggttaacga aatgctcgac tattttgaaa tcgggcatgt taagatactc atcgaagact
    28681 caggcgcatt gtcttatatt cttgctgccc gccttgaagc agccgtcagg atggtgaagc
    28741 catcggtaaa ggaatacctc ttgccgatgc ttcccgaaaa ccagtatcat acatcacgtg
    28801 aaaagaacag gttttcgagg ctttacctgc ccggcaacac gccgagttta gctattaatg
    28861 cgggtattca caaacccgac ggcattatcc tcgacctgga agatgctgtg gccccggcca
    28921 gaaaattcga agcccggtat ctggtcagaa acacgcttcg cagcgttgat ttctatggcg
    28981 ccgaacgaat ggtacgtata aaccaggtgc cagcgggcct cgacgacctg gaatacgtca
    29041 ttcctcacaa tgtcaacctt ctgcttgtcc ccaaatgtga aaacacggaa cagctaaggc
    29101 ttgtaaacga aagagtggcc gccatcaagg cgcggaccgg caacaaagag cctgtatggc
    29161 tgatgccgat catcgaaagc gccctgggcg tgatgaaggc ttttgaaata gcacaatgtg
    29221 aaaatgttgt gtccctggcc atcggactcg aagattacac cgccgacctg ggcgtgaaac
    29281 gtacccagga aggaaacgaa tcgttctttg cccgttgtat gctggtgaac gcctgcaaag
    29341 ccgccggaat ccaggctatc gactcagtgt acagcgatgt gggcaacatg gaagggctaa
    29401 aagaaaatgt tctccgctca aaagcattgg gatttaatgg tatgggatgt atccatcccc
    29461 ggcagataaa ggtgatccac gaaaacttcg cccccgataa ggatgaaatc gaaaaagcaa
    29521 agaaaattta tcttgctttc caggatgcca cggaaaaagg actgggcgtt gtttcactgg
    29581 gaacgaaaat gatagatcct cccgtggtaa agcgtgccgt taatacaatc gaactggcgc
    29641 tgaagacggg caaactgcct gtggattgga aagaacaaat gttaaccgaa aaagcctagc
    29701 catgaaaaaa tgcgataatt tcgttataaa tgctgccgga cgaaaagttc cgactctcgt
    29761 gaacggacag gaagttgtag ccttcaaagg attgcgtcag caccggccag ccgggcggaa
    29821 atatgcttcc cccattccca cctgtattga ttaccccgac gacggaaaca agatagtccc
    29881 ctcactggcg gaagccttga aaaagtgcgg gctgaaagac ggaatgacca tcagcaccca
    29941 tcaccatctg cgcgatggcg acctggtgag taatatgatt ttcgatattg cccatgatat
    30001 gggcgtcaga gacctggttt ggctcccgtc ggcatcattc ccctgcaacg atcccctgat
    30061 caaatacctt gaagacggga ccattaaccg tatcgaaggt tccatgaacg gcccgctcgg
    30121 gaaattcacg accctgggag ggatgaaagg ctgtgctgtc ctccgttcac atggcggacg
    30181 cgttcaggct atccaggatg gggaagtcca tattgatatt gccgttattg ccgctccgac
    30241 cgctgatgcc tttggtaatg caaacgccct taacggaaaa tctgcctgcg gaattttagg
    30301 atatgcgctt gccgattaca tgtatgccga caaggtcatc attgttaccg acaacctgat
    30361 ccccttcccc tgcatgccga tgcaggtgca gggcaattat gttgattacg tagtgcaggt
    30421 tgatcagatc ggtattcccg aaaaaattgt atctggaacg acggaagtaa caaaaagccc
    30481 cgaccgcctt ctcctggccg agtggaccgc ccggttctgc gacgaggctg gccttctgca
    30541 cgatggttgc agcatccagt caggtgccgg cggaacatcc ctgtcggtgg gtatctattt
    30601 tgaagagatg ttgaaggcgc gcggacagaa agcccgtttt gctttcggtg gcagcaccaa
    30661 atatctggtc aggatgctcg aaaacggggt gctggattac attctcgatg cccaggcctt
    30721 tgaccttgaa gccgtacgtt cagcccgtga aaatccgaac catattgatt tgtcggtgct
    30781 ccaggcatac aactatcaca gcaaaggcaa ctatacctct atgaccgata tcatgatcct
    30841 gggcgccacc gaaattgata caaactttaa cggaaacgtg gttactcatt cggatggctt
    30901 gctgcttcac ggaatcggcg gatggcaaaa ttgtttgcat gccagaaacg tcatcattcc
    30961 cattcccctg ttccgtgacc gtatccccgt gctggtcgac aatgtaacga ctttatgcgg
    31021 tcccggcgag ttgatcgacg ttatcgtgac ggaacgcggt atagccatca acccgaaacg
    31081 taccgacctc atcgagaaga tgaaaggttc ttcattacct atcaaaacta tccaggaact
    31141 gaaagaagaa gctgaaaaga tttgcggaaa acccgccaga ccccgcttca ccgatgagcc
    31201 ggttgccgtc attaaatggg tcgacggaac cgttattgat acggtgtgga aactggagaa
    31261 gtaaaaggtc aaaagtttac ctgcctgggt gaggttgtgg gcaagatggc aggcctgcct
    31321 gcctggatga ggttgtgggt aaggcggcag ggcaaaggtc aaagggcaaa gggcaaaggg
    31381 cacggagcgg ggagcataac ggaggacgga gaactttacc tgccttgcgc aagactgcgc
    31441 gtggcaggca ggggaggacg gagaacggaa ag
//
LOCUS       DAOPYV010000034        30681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_1435_length_30681_cov_12.122837, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000034 DAOPYV010000000
VERSION     DAOPYV010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30681
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1435_length_30681_cov_12.122837"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..2930
                     /locus_tag="PKI35_06150"
     CDS             153..2930
                     /locus_tag="PKI35_06150"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX44002.1"
                     /translation="MQKKRRRLSLTNIIFIHILIIVTVSMIAIGTYSIIRKIQTYNHD
                     VSRLRTEFPMRQKNELKFRVLEAKEYINWVVTYPEKSINSHLPKLSMRYARLMENSKI
                     SKTGSSYFIPQKIKDTLNYANLNCVIKMAVYDSAFQPVYLPELNDLPGEVQYMPLIEK
                     TISAIKKEKSETGFYPAIRSISEKSGYAAMASVNNGDFWVAAVFSGNDPGKALQEIVL
                     DSLSRVKFEDNEYFIINSADGNAYLTRTIWDKDPKNILTGDDENWKKIFRNELLVARH
                     PGGGYILYNWKRKYTDSLSTKISYFTGISQWNWIIGTGKHLDALIPLLEKEKNQLRAE
                     IRADILSKAIFAIIIFAFIIFLVQYFSRMISSNLRHFNLFFQNAVHGNQHIDEESIHF
                     REFGSLARSANLMIEEREKMRAILAEQQSRLRNIIDSVPDLIFFKDTDSRFLGCNKAF
                     EKFVGKKEAEIIGKTEFDLFTPAQAEMYLASDKHLMLTGEIARNKEWNRLKEGYSILL
                     DTLKTIYRDEEGKILGIIAISRDVTEMEETRQRLVLAKNKAEESDRLKTAFLANMSHE
                     IRTPMNAIIGFSDLLADDDLTPEEKQDFIAKIKLSGENLMNLINDIIDIAKIEAGQLK
                     IMESTCNVGKLLETLHGTFSEIRNQQKKNAIELRCYKEPDEGDLIIITDPLRLQQVLT
                     NLIGNALKFTEQGYIEFGCKRKNGFLEFFIKDTGIGIPQDKHDILFQRFSQVDASKTR
                     KFGGTGLGLAISKNIVELLGGSIWFESEAGQGSTFYFTIPYKPSSGGELANSSKRPAT
                     DYDWSDKTILVAEDIDENFMLIEAAFRRTGAKLIHACDGLAAVNEAKSNDSIDLILMD
                     IQLPIMTGYEAIRNILIAKPDMPIISYTAYALANEREKSIAAGCVDYIAKPIRHEVIL
                     PIINKYLKPGT"
     gene            complement(2990..3169)
                     /locus_tag="PKI35_06155"
     CDS             complement(2990..3169)
                     /locus_tag="PKI35_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNX44003.1"
                     /translation="METNDLVFQTLASSKEPLKAGDIAEKAGIDKKDVDKAIKVLVAS
                     GKIESPKRCFYSAKK"
     gene            complement(3181..4434)
                     /locus_tag="PKI35_06160"
     CDS             complement(3181..4434)
                     /locus_tag="PKI35_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNX44004.1"
                     /translation="MAILKAFKGLRPPKEIVKKLASRPYDVLNSSEARIEAEGNPYSL
                     LHVTKAEIDLEPGIDEHSQAVYDKVVVNFDRFQKEGWLVQDNEEKLYIYAQTMEGRTQ
                     YGIVGCCHIDDYFNNVIKKHELTRKDKEDDRMIHVRITDANVEPVFFSYPSHKELDDM
                     IGKYVKENKPEYDFVADDGFGHHFWVINDSRMISRIVEIFRDEIPSLYVADGHHRTAA
                     AARVGQEKKAHNPSHTGKEEYNYFMAVIFPDDQLKIIDYNRVVRDLNGLTEKEFLDKL
                     SEVFTIEKKGTEIFKPCCLHNFSLYLGGNWYSLTAKPGQYNDNDPIGILDVTILSNLV
                     LDRILGITDLRTSKRIDFVGGIRGLGALKKRVDSGEMKAAFALYPVSMKQLIDIADSG
                     NIMPPKTTWFEPKLRSGLVIHKLDS"
     gene            complement(4611..5021)
                     /locus_tag="PKI35_06165"
     CDS             complement(4611..5021)
                     /locus_tag="PKI35_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX44005.1"
                     /translation="MDSELEKTTTFFRFEDLRVYHKSLDYIDWVYRKVNKFSEDGSST
                     MSRRFFDSSQAIALHIAEGSARNKSQFIYYLKMAKSSVRECVVHTAMASKQGFLSHTE
                     EEESRNQLMEMTKMLGALIGSLQRSAGITDTDDD"
     gene            complement(5286..6212)
                     /locus_tag="PKI35_06170"
     CDS             complement(5286..6212)
                     /locus_tag="PKI35_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX44006.1"
                     /translation="MKKVLVATEKPFAKAAVEGIKGIIEGEGHEMILLENYTTKAELI
                     AAAARADAMIIRSDIVDKEVIAASINLKIVVRAGAGYDNIDLEAASGAGIVAMNTPGQ
                     NSNAVAELAVAMMVYAARNNFNGTSGTELKGKKLGLHAYGHVSKAVAAIAKGFSMEVY
                     AFDPFVDKKAMISDGIIPVDNVNELYSQCQYISLHIPATPQTKKSIGFELLSKMPKGA
                     TLINTARKEVIDEEGLLQVFAQRPDFKYMSDVEPDCKSDFAEKYTGRFFFTPKKMGAQ
                     TAEANNNAGLAAARQINSFFATGDTTFQVNKK"
     gene            complement(6288..7358)
                     /gene="serC"
                     /locus_tag="PKI35_06175"
     CDS             complement(6288..7358)
                     /gene="serC"
                     /locus_tag="PKI35_06175"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNX44007.1"
                     /translation="MKKHNFNAGPCILPRIAVENTANAVLDFNGIGMSILEISHRSKD
                     FQAVIDEAVALFKEVLAIPEGYQVLFLGGGASLQFCMVPYNFLNKKAAYLETGVWAKK
                     ALKEAKLFGEVVTVASSSDKNFSYIPKGWSIPTDVDYFHITTNNTIYGTEIREDFNSP
                     VPLIADMSSDILSRPVDISKYAMIYGGAQKNAGPAGVTFVIVREDMLGKVDRAIPTML
                     DYRTHIKDGSMFNTPPCVNIFTLKETLKWLKSIGGVKKMEELDKAKAKLLYDAIDSSK
                     MFVGTVEKDSRSIMNICFVMKEQYKDKEDAFMEFAKAHGMVGIKGHRSVGGFRASCYN
                     ALPVESVQALIDCMHDFEKENA"
     gene            complement(7489..8421)
                     /locus_tag="PKI35_06180"
     CDS             complement(7489..8421)
                     /locus_tag="PKI35_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44008.1"
                     /translation="MKNSLFNTFLVLAFIALSLVSYGQQADFAALEDSLAKMSIRIWQ
                     QKDDQHVKAYSKKFAEQLRQVMELPGSFDYPFDSLKEIGRITSEDKRFRIFTWNCRSS
                     DGKFSYDGLLVTGGEHPAVFPLTDVPGKQNLANADFSTPEHWYGALYYKIIPSKSGKK
                     NIYLLLGWDGIDDNMNSKLIEVLSFAPDGSPVFGMPVFHTPKGVVPRVIIKYAENASL
                     VLRYDYQTLLLPRGKGVKRKSMWMIVTDHLVPMTPQLEGQYEYYVPSGDSYDAYFFNK
                     GKWSFAEGVEVGNPPTGKNASEHKPVNYNLFPPK"
     gene            8566..9675
                     /locus_tag="PKI35_06185"
     CDS             8566..9675
                     /locus_tag="PKI35_06185"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HNX44009.1"
                     /translation="MITLENRIRAFIKLGNLMGQASANLKPAANEPCYTAFENLIKAM
                     KQAYIQNPWFTPDNIAASFRAWSYSLSEQKIRQWLNPYVTLLADDPEPRKTAVIMAGN
                     IPLAGLHDLICVLISGNSLLGKVSADDACLLPAIAGLLCAIEPGFGPLIEFSTEKMSG
                     FDMVIATGSNNTSRYFEQYFAAYPHIIRHNRNGIAVLNGHETPAELENLGHDIFSYFG
                     LGCRSMSMMYVPENYDFSLFSGAVAVYRDRMIMHHKYMNNYTYRKTIFMLNNTPFIDG
                     GFFLACENQDFISPISVIHYFHYKSLPALAEYLENEHDNIQCILSNISTLQHAVPLGK
                     GQQPELWDWADGTDTLKFLLENMPPFEKNEQGTVK"
     gene            9748..10023
                     /locus_tag="PKI35_06190"
     CDS             9748..10023
                     /locus_tag="PKI35_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNX44010.1"
                     /translation="MKKDIHPKEYRLVVFKDISNDYAFMTRSTVATKETIKWEDGKEY
                     PLVKLEISNTSHPFYTGKIKLVDTAGRVDKFRQKYEKYQKLRKQSEQ"
     gene            10251..12707
                     /gene="lon"
                     /locus_tag="PKI35_06195"
     CDS             10251..12707
                     /gene="lon"
                     /locus_tag="PKI35_06195"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNX44011.1"
                     /translation="MKANKDYPILLSNILDDETEFIPLLSAEDEEHINAESVPEVLPI
                     LPLRNTVLFPGVVIPITVGRDKSIKLIKEHYKGERIVGVVAQKDPDVEDPGFDDLHGI
                     GTVAYIIKILQMPDGSTTVIIQGKKRFELGELVQSEPYFKASVKAYGASPEGKKKKDK
                     TFEALMSSIKDLATQVVQQSPNLPSEAGFAIKNIESPVFLVNFVASNLSIDLSEKQKL
                     LAIPDFNEQANTVLGLLTREIQMLELKNQIQNKVKIDMDKQQRDYLLNQQLRLIQEEL
                     GNNPHEQQINELKTKASNKKWPENVSQVFEKEIAKLSRMNPAAMEYSIQMNYLEVMTD
                     LPWDEVTDDNFDLKNAQRVLDRDHFGMEKIKERIIEYLAVLKLKRNMKSPILCLVGPP
                     GVGKTSLGKSIARALGRNYIRISLGGLHDEAEIRGHRRTYIGAMPGRIIQSIRKAKSS
                     NPVFVLDEIDKVAGMTVNGDPSAALLEVLDPEQNSAFYDNFMEVEYDLSKVMFIATAN
                     TMSTIHPALRDRMEVLDLSGYLLEEKIEIAKRHLVPKQLSEHGLTSKQVTFTKPALQK
                     IIEDYTRESGVRNLEKVIAKLIRNRARYVAMNEKIPASVTPADIEKILGSPHFQQEKS
                     LSNNIAGVVTGLAWTATGGEILFIEVSLSAGKGNLTLTGNLGDVMKESATLAYEYLKA
                     HSDLLDIDPGVFEKWNVHIHVPEGATPKDGPSAGITMFTALASAFSQRKVRSKTAMTG
                     EITLRGRVLPVGGIKEKILAAKRAKITDIILSEDNRKDILEINPIYIKGLTFHYISEM
                     IEATNLALLNEKVGHPLSLS"
     gene            complement(12739..14730)
                     /locus_tag="PKI35_06200"
     CDS             complement(12739..14730)
                     /locus_tag="PKI35_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNX44012.1"
                     /translation="MKKFTILFLFLGLYGILSAQDFSTMSGSQLCSYRKSHIQQLPDL
                     RMLSPNAPVHSFDVLNYKFDLDLYNCYISPYPKSFTGSVILTFEADSVIDHIVLNAVN
                     TSLEIDSVGMNGVSFTHASDLLNIVLDRTYNPGEQAAVKVSYHHKNVSDGAFYVNSGF
                     LFTDCEPEGARKWFPCYDHPSDKATTDLTARVPLNVKLASNGRLQDSTVNGNILTYHW
                     ISRDPVATYLTILTSRAGYNLDIVNWTNPNTGEVTPMRFYSNPGENPDGMESIFPDLV
                     TFYSQEYGDHPFEKNGFATLNSDFTWGGMENQTLTSLCPNCWTESLVAHEFAHQWFGD
                     MITCATWADIWLNEGFATWSEAHWIENTDGYTAYKNEIKNNASTYFAYGSPWPISDPA
                     WAVTTPSVDILFDYGVTYMKGSCVLHQLRYVMGDSAFFPAIKAYATDTVDFKYKSATI
                     YDFKEKIESVSGQELDYFFDEWIYTGGHPKYQNVYDIKPYGNGWRVTFTCRQAAAPIY
                     WQMPVELRITFKDQSDTLVKVFNSYNDQIFTFDFTDKEPRTVFFDPNNQIVLKKGSTI
                     VSTDEPLVAENKSYIESISPNPAGSQTTIAFHIEKASDAKLMLFDMAGKAVASMMDGY
                     LNPGDHTFNLQTSGLASGIYTCRLQTVDSVHSVKLVVSK"
     gene            14830..16467
                     /locus_tag="PKI35_06205"
     CDS             14830..16467
                     /locus_tag="PKI35_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX44013.1"
                     /translation="MFRKTAIILILSLLCFACHRHRPGDAGKTVFRYNESAGITSLDP
                     AFASNQANIWAVNQLYNGLVQLDDALKVRPCIAKRWEISNDGLIYTFYLRNDVFFQDS
                     PIFKGGKGRKVVASDFVYSFRRIADPKIASPGAWVFGQVKSANGLPEFRAVDDTTLRV
                     FLSAPFPPFLGLLTVQYCSVVPHEAVEQYGADFRRSPVGTGPFRLKMWKEGVKLVLVK
                     NENYFETEGKRRLPFLDAIAVTFIVDKQSAFLEFVKGNLDFMSGIDASYKDELLTRDG
                     DLNPKYRNKISLVTSPYLNTEYLGILVDTGNKLVRTTPLRLLKIRQALNYAFDRKKMM
                     RYLRNNIGIPGVAGMVPPGLPFFDQAKVYGYDYNPERARQLLAEAGFPGGKGLPSITL
                     TTNASYLDLCKYIQSRLNETGFDIKIDVTPPGTLRDMIALSKVNFFRGSWIADYPDAE
                     NYLSLFYSPNFCPEGPNYTHFADKDFDLLYRRAAAEANDSIRGRLYMEMDNLVMRQAP
                     VIVLYYDQVLRFTQKNIHGLTPNPLNLLTLKHVRKTK"
     gene            16529..17008
                     /locus_tag="PKI35_06210"
     CDS             16529..17008
                     /locus_tag="PKI35_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HNX44014.1"
                     /translation="MLDDDWMLITAGNLSAFNTMTASWGGFGVLWNKPVAYVFVRPTR
                     FTFGFMEQSELFTLSFFEEKYRHILQYCGKVSGRDTDKIAGAGLTPLKSPSGAVMFKQ
                     SRLVFDCRKIYTSDINPSDFLDPAIEKNYPDHDYHRMYIGEITAYLTDQDISGTPGW"
     gene            17112..17669
                     /locus_tag="PKI35_06215"
     CDS             17112..17669
                     /locus_tag="PKI35_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44015.1"
                     /translation="MGFLNNIRQKLACRRLNQEHQKLARIRKSTSLENARAIGILYYL
                     PDEDTYKQVEEFIRLLTDMNLKVRVACYTDNKIPPHYFIPKLLQDIITPKDLSWIGVP
                     EKLFVPDFLAEKFDILIDLSLTDHFPLLYLAALSKASLKIGRYDASRQEFFDMMIHTS
                     PDTSLQEFTEQILHYLKMFNNTNKA"
     gene            17666..18562
                     /gene="dapA"
                     /locus_tag="PKI35_06220"
     CDS             17666..18562
                     /gene="dapA"
                     /locus_tag="PKI35_06220"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNX44016.1"
                     /translation="MNSKFKGTGVALVTPFHKQGTIDFSSLGKLIEHTIEGGVNYLVV
                     QGTTAETATLTKEEKHAVSQFVIDTVDKRVPLMLGVGCNNTQEVVNTLRTASYEGFDA
                     VLSVTPYYNKPQQRGLYLHYKNIAAVSPLPIIMYNVPGRTSVNMKAETTLELAHEFDN
                     IIGVKEASGNIEQVMDIIRQKPKDFLVISGDDLLTAPMLTMGADGLISVAANAFPKEI
                     SDLVMYGMKGDLKKMREIHYRMTDFIKAIFADGNPSGIKAALEIKGLISNNLRLPLVK
                     IEKNLYNTLATLIQEIEPGNLS"
     gene            18588..20921
                     /locus_tag="PKI35_06225"
     CDS             18588..20921
                     /locus_tag="PKI35_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HNX44017.1"
                     /translation="MKKIILFACITLCVTVNVWAQQNAAEISKAYEAINARGEVYFSF
                     NISDRNEIGALTKIISIDDVKGNTVYAYANSKEFNAFRQLGYDFKALTAPSLMFEAAM
                     SNNPDQVLGWDYYPTYSAYESIMSQFAADHPDLARLITIGTLPSGRKLLAIKISDNPD
                     VPENEPEFLYTSSIHGDETTGYPMMLHLIDYLLTNYGTDDRITDFVNNMEIYICPLAN
                     PDGTYHGGNTSVNGAIRYNANNVDLNRNYPDPEPLDPGNPNYDHMHPDGLAWQNETVA
                     FMDFASQHHFTMAANFHGGVEVVNYPWDTWAKLTADNNWWVYVSREYADTVHVNAPNG
                     YMTYLNNGITNGYAWYRITGGRQDYMNYFKHCREVTIEISDTKLVPASQLIPHWNYNY
                     HSLLNFMNESLYGLHGIVTDTVTGLPLEAKIFITNFDKDSSHVYTDPAVGDYHRLLKA
                     GTYNVTFSAPGYISKTYQVQIADMQKKTLDVQLFDGALTASFSADTTNIYPGTEVHFT
                     DHTLGYPASWQWEFEGGTPATSTQQNPVITYNNYGSFNVKLIVSRTDGSDTLLMQNYI
                     SVAPSYLQGNYYFTTCHGNYFDSGRENSNYSDNELSAITFVPGESDKKITIYFVDFDV
                     EQSANCDNDRLTIYDGPSTTDPVIGNYCGTSIPESVTATNAGGALTVLFRSNSSVTGA
                     GWNAYVVCSPNTGIAEDEDASIKLFPNPALNGSATLISPAIINSLQITDCLGKVVYRL
                     NPGANNVQIPSGLAAGLYFVAVQTGEQWYVKKLQIMR"
     gene            complement(20986..23205)
                     /locus_tag="PKI35_06230"
     CDS             complement(20986..23205)
                     /locus_tag="PKI35_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HNX44018.1"
                     /translation="MPQKAEITNLFNETCALVFDNRLKEALSRMGRMMEGLHVADFTM
                     QYENLDQTYRMLLEYTFRGVPDPHRDRIYRSLKISILELADMVQQKAFEMTGMNTYSL
                     KMQLEKEKELAKEDAARNFDTLSFDKELERVLSETELQAQGETAKGRLHEITPKLFQM
                     IWLSDKYTDTDIRLIEAIRVSEELPWYEKCLVVSAITLSLLNCYDTEKFLLLFKFYEQ
                     RQNEVWQRALSAIVIAIYYYDRRIGFYPAISAKILEYQEDEQFYRDMGQVLIALFKAL
                     ETEKVTRKFQEDISPDIQKIEEKIREKLDIDNILESDIIEDKNPDWETLFEDSPGLLD
                     KIEKMSNMQMEGSDLFMGTFAMLKHFDFFRDISNWFRPFYKENEAVRSSVTGEGEKGG
                     AFLEGMENSFYMCNSDKYSFCFNISHLPANQRNGIIGLFNMEAESLRELKDEENLLAN
                     PRRESYIFTQYVQDLYRFYKLHPFRSDFRDIFAMKWDIAGSALFEIIKSNHALVNDLA
                     EFLFEKERYNEAIPLLNHLVSQPEPSGALIGKIAYGYQRLGDYEKALGFYRQSEMFDS
                     NRLWSVKKIIFCLRQLNKYEEALKWCNEALSIDPDDVYINTMAGNCLFDLKNYQEALE
                     HYFKVEFLAPDNKKVLRPIAWCSFVSGKLEIARNYLQTILESEPTANDYINAGHVEYC
                     LAFKQKAMEYYMQALRQPGMSVARFAKVIQNDTPVLEANGVKSEELPLLVDYIRFLI"
     gene            complement(23228..25270)
                     /gene="ligA"
                     /locus_tag="PKI35_06235"
     CDS             complement(23228..25270)
                     /gene="ligA"
                     /locus_tag="PKI35_06235"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNX44019.1"
                     /translation="MTENPVAERIQFLTDEINRHNYLYYVEAQPEISDFDFDMLLREL
                     TDLEKANPELALPDSPTQRVGGYVTKEFESYEHKIPMLSLGNTYSEEELRDFDQRVRK
                     AVGDNAEYVCELKFDGVSISLNYQKGLLVRALTRGDGVRGDDVTANIRTIRSIPLRLK
                     GTGYPDEIEVRGEIIMPRSAFDAMNAERIEIGEPPFANPRNSTAGSLKLQDSSEVAKR
                     RLDNFCYFLAAEGLPFMTHSESLMALKSWGFKISDMTRVCRDMNEVWNYILEIGKQRE
                     KLPYDIDGAVIKVNSTEYQQMLGFTAKSPRWAIAYKYKAERAATRLLSIDYQVGRTGT
                     VTPVANLQPVKLAGTIVKRATLHNADVIEKLDVRVGDTVYVEKGGEIIPKIIGVEMAK
                     RDLFSAPQEFITHCPECGTSLVRNPGEAAWYCPNEDGCPPQIKGKLEHFISRKAMNIE
                     SLGEGKVEMLYDSGLVKDVADLYDLTGEKLIGITKVIGNGDQKEKKVSIREKSAENIL
                     NGVESSKNVPFERVVYALGIRYVGETVAKKLARHFGSMDALMAAGTEELLTAEEIGTK
                     IAASIQDYFSNPKHLEIIARLKSAGIRFELSRSANANLSDKLAGKVFVVSGVFSRSRD
                     EIKSLIEAHGGKNAGSVSKNTTFVLAGENMGPEKQKKAAQLNIPIISEDELMRMIQ"
     gene            complement(25267..26820)
                     /locus_tag="PKI35_06240"
     CDS             complement(25267..26820)
                     /locus_tag="PKI35_06240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNX44020.1"
                     /translation="MMLTIKQIKRLREYRPLLFILLPALLLRLLSAIFSRGFGMHDDH
                     FLVIEPSYWWSQGINYQEWLPMNGLHPHPDGHSLFYSGLHYLFFVVCNGAGIADPQVR
                     MLLVRVIHALFSLLTVYFAYRITEKISGKKEAFTAALLTAFFWFMPFLSVRNLVEIVC
                     IPFLMYGAWLIISAYDARKPLWRYFAAGLVMGLAFSVRFQTASFIAGLGLAMLILKLW
                     KPTLVFGIGVLASVLPVQGLIDYFIWGAPFTEFAEYVRYNIQAANDYIIGPWYNYILL
                     LAGILIPPVSIFLLTGFFASWRKQLLLFLPAFVFLVFHSVFPNKQERFIFPVIPFVIV
                     LGTAGWSAMMQKSGFLKRHRGFISGSWKFFWIVNIILLLAISFSYSKKSRVEAMTYLS
                     GYKDVKMILLEDSRHNSAKMVPVYYMGQWADVMEKSKTGLRMNFRRRFTDADSLKYAR
                     FVLFFEKDSLEGRVNMLRTYLPGLEYETTIRSGMVDRFMHWINPVNLNQDIYIYRNKA
                     YFPEKKKER"
     gene            26963..29008
                     /locus_tag="PKI35_06245"
     CDS             26963..29008
                     /locus_tag="PKI35_06245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNX44021.1"
                     /translation="MKRIFTFCSLVLPLFGLLSPVLTFSQERYTPELLWKLGRVSEAQ
                     VSPDAGRILYGVTWYNLEANKGNRDLYMLPVAGGRPVKVTSFQGSESNGIFRPDGKKI
                     AFLSSESGSSQIWEANTDGTNPVKISNEDGDITGFAYSPDGSRILFTKRVKLDKSPND
                     IYPDLPKAKARIEDDLMYRHWDSWSDYCYSHIFVAGYNKGVIGKPTDIMPGERYDSPM
                     LPWGGMEQISWSPDGNSIAYVCKKMTGLQYAVSTNSDIFVYDIASGKTRNISESNPGY
                     DQDPAFSPDGSKIVWRSMQREGFEADKDRIMIFDVKSGTMQDYSAGFDQSSKNFTWAA
                     DGKSLYFISGHHGTEQIYRLDIATKAIKQISSGMHDYLSIALSGNDVIGIKQSMLMPA
                     EIYKIDAKGAETQLTFTNKEILKNVKSASVQERWVTTTDGKKMLVWVILPPDFNPARK
                     YPALLYCEGGPQSMVSQFFSYRWNFQMMAANGYIVVAPNRRGLPGFGQEWNDQISGDY
                     GGQNMKDYLTAIDTLAAEPYVDKDRLGAVGASYGGFSVLWLAGHHEKRFKAFIAHCGM
                     FNLEDQYLTTEEMWFVNWDLGGPYWEKGNPKADHSYANSPHRFVDQWDTPLMVIEGEN
                     DYRIPFTQGMAAYNAAKLRGIPARYLHFPDESHWVLHPQNSVLWQREFFRWLDQWLK"
     gene            complement(29178..29660)
                     /locus_tag="PKI35_06250"
     CDS             complement(29178..29660)
                     /locus_tag="PKI35_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNX44022.1"
                     /translation="MFETSVIISEIHSLTEAWEPVLASLPAGIIMQRRNRQNRTIKQI
                     LGHLVDSASNNIHRIVHLQYRQSPLSFPNYATNGNNDRWIAIQDYQNEDWNDLIRLWK
                     YTNLHLCHVIRNVDHGKLENEWMSGPDSMITLEEMIRDYPLHLKLHLSEIDELINSPS
                     "
     gene            complement(29895..30284)
                     /locus_tag="PKI35_06255"
     CDS             complement(29895..30284)
                     /locus_tag="PKI35_06255"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNX44023.1"
                     /translation="MSGSKQDLISYRLKRAKETLDDATILANNEKWNSAINRLYYSAY
                     YAVMALLLKHDLNPTTHNGAKHNFSEHFIKSGIISKDYGKFYSQLFSWRQKGDYDDLF
                     DFTEEKVMPYFKPVEDLINIIESLTKE"
     gene            complement(30281..30601)
                     /locus_tag="PKI35_06260"
     CDS             complement(30281..30601)
                     /locus_tag="PKI35_06260"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNX44024.1"
                     /translation="MTKKDKILSEIIRLTNAADPKAEVYLYGSQARGNPHRLSDWDLL
                     ILLNINNIPFALETSLMDQIYDIELESGEIISPLIYSKKDWEEKHYITPLFENIKNEG
                     IRIQ"
BASE COUNT         8357 a         7427 c         6878 g         8019 t
ORIGIN      
        1 tgagcattaa aaacaaaaaa ttaaaattat tccggtttac cggacaacaa tgactatttt
       61 tgtaaaagag gcttctgaat agtaaaattt cgtgtacatt tattcattgg aaatcagtca
      121 cagcaatgtc cccgaacaat caaactgaaa gagtgcaaaa aaagcgccgt cggctaagcc
      181 tgacgaatat catcttcatt catattctga ttatcgttac tgtctcaatg atcgccattg
      241 gcacatattc aataatcaga aagatacaaa cgtataatca cgatgtaagc cggttaagaa
      301 ccgagttccc gatgcggcaa aaaaacgaac ttaagttcag ggtgctcgaa gccaaggaat
      361 atattaactg ggttgttacc tatcccgaaa agagtataaa cagtcacctc ccgaaactca
      421 gcatgagata tgcgcgtctc atggaaaatt caaaaatcag taaaaccggc agttcttatt
      481 tcataccgca aaaaattaaa gataccctga actatgcgaa cctgaattgc gtgatcaaaa
      541 tggcggtata tgactctgct tttcagcctg tttacctccc tgaactcaat gatctgcccg
      601 gcgaggtaca atatatgccg ttgatagaaa agactatatc ggcgattaaa aaggaaaaat
      661 cagaaacggg tttctatcca gctatccgga gcatcagcga aaaatccggc tatgctgcca
      721 tggcttccgt caataacggg gatttctggg tggcggctgt attttccggg aacgacccgg
      781 ggaaggcgtt gcaggaaatt gtccttgata gtctgagccg tgttaaattt gaagataatg
      841 agtatttcat tataaattcc gcggacggaa atgcctatct cacacgtacc atctgggaca
      901 aggatccgaa aaatatcctg acaggagatg atgaaaactg gaagaaaata ttccgaaatg
      961 agttattggt agccagacat cccggcggcg gatatattct ttacaactgg aaaaggaaat
     1021 acaccgattc cctttcgacc aagatttctt atttcacagg catcagccaa tggaactgga
     1081 tcatcggtac cggtaaacat cttgatgcgc tcattccgtt actggaaaaa gaaaaaaatc
     1141 agcttagggc agaaatacgc gccgacatct taagcaaggc gatcttcgcc atcatcattt
     1201 ttgccttcat aatttttctc gttcagtatt tttccaggat gatttcgtca aacctcaggc
     1261 attttaacct gttttttcag aatgccgtac atggaaatca acatattgac gaggaaagca
     1321 tccatttcag ggaattcggg tcattggcca gatcggctaa cctgatgatc gaagaaagag
     1381 aaaaaatgcg ggccatactt gccgaacagc agtcgcggtt gcgaaatatt attgattcgg
     1441 tccccgacct tattttcttt aaagataccg acagccggtt cttgggatgt aataaagcct
     1501 ttgaaaaatt cgtcggaaaa aaagaagcag agatcatcgg aaagacagaa ttcgaccttt
     1561 tcaccccggc ccaggccgaa atgtacctgg cctccgacaa gcatcttatg cttaccggag
     1621 aaatagcgcg gaacaaggaa tggaataggt taaaggaagg ttacagcata ttgctcgata
     1681 cattgaaaac catttaccgc gatgaagaag gtaaaatttt gggcattatc gctataagcc
     1741 gtgatgttac cgaaatggag gaaacgcgcc aacggctcgt gttagctaaa aacaaggcgg
     1801 aagaatcgga tcggctgaaa acggcatttc ttgccaatat gtcgcatgaa atccgcactc
     1861 ccatgaatgc cataatcggt ttctccgacc tgctggccga cgatgacctc actccggagg
     1921 aaaagcagga cttcatcgct aagatcaaac tatcgggtga aaacctgatg aacctcatca
     1981 atgatatcat tgatattgca aaaattgaag ccggccagct aaaaatcatg gaatcaacct
     2041 gcaacgtggg taagttgctt gagacgttgc acggcacttt ctctgaaatc agaaaccagc
     2101 agaaaaaaaa cgctattgaa ctgcgctgtt acaaggaacc tgatgaaggc gatttaatca
     2161 taattaccga cccgctgcgg ttacaacagg tgttaacaaa cctgattggc aatgccctta
     2221 aatttacaga acaaggatac attgaatttg gctgtaaaag gaagaatggc ttcctggaat
     2281 ttttcatcaa ggataccggc atcggtatac ctcaggacaa acatgatata cttttccagc
     2341 ggttttcaca ggtcgacgct tctaagacaa gaaaattcgg cggcacagga ctgggactgg
     2401 ctatttcgaa aaacatcgtg gaactattag gcggcagcat ctggtttgaa tcagaagcag
     2461 gccagggatc cactttttat tttacaattc cttataaacc ttcgtcgggt ggagagttgg
     2521 caaattcttc gaaaaggccg gctactgatt atgactggtc ggataaaacc attctggtag
     2581 ccgaggacat tgatgaaaat ttcatgctga ttgaagcggc attcaggaga accggagcta
     2641 aactgattca tgcctgcgat ggtttagccg ccgtaaatga agcaaaaagc aacgattcca
     2701 tcgaccttat cctcatggat atacagttgc ctatcatgac cggctatgaa gccatccgga
     2761 atatactgat agcaaaacct gacatgccga tcatcagcta tacggcctat gcccttgcca
     2821 atgaacgcga aaagagtatt gctgccggct gtgtggatta tatcgcaaaa ccgatccggc
     2881 atgaagttat cctgcctatt atcaataaat atttaaagcc cggaacctga aaaaggttcg
     2941 cgggctttag cacaaaaaag taaaatgcgg ccgtcagact gccggaattt tatttcttcg
     3001 cagaataaaa acagcgcttc ggcgattcaa tttttcccga cgcaacaagg actttgattg
     3061 ctttatcgac atctttcttg tcgataccgg ctttctcggc gatatctcct gctttgagcg
     3121 gctctttcga cgaggcgagc gtttgaaata ccaaatcgtt tgtttccatg atatgaagtt
     3181 ttaagaatcc agcttatgaa ttaccagtcc gcttctcagt ttgggttcaa accaggtagt
     3241 ttttggaggc ataatgtttc ctgaatcggc gatatcaata agttgcttca tcgatacagg
     3301 atacagcgca aaagcggctt tcatttcacc gctgtcgaca cgttttttaa gcgcgcctaa
     3361 gcccctgatg cctcccacga aatcaatacg tttggaggta cgaaggtcgg ttatgcccag
     3421 gatgcggtcc agcacaagat tggataagat tgtaacatca agtattccga tcgggtcatt
     3481 atcgttgtat tgcccgggct tggcggtaag tgaataccag tttcctccca ggtaaaggct
     3541 gaaattatgc aggcaacagg gtttgaaaat ttcagtccct tttttttcaa tcgtaaaaac
     3601 ctctgaaagt ttatcaagaa attctttttc agtgaggcca ttcagatccc tgacaaccct
     3661 gttgtaatcg ataattttca attggtcgtc gggaaatata acagccatga aataattata
     3721 ctcctcttta cccgtatgag aaggattgtg agcttttttc tcctggccga cccgtgctgc
     3781 ggcagcagtg cgatgatgcc cgtcggctac atataaactc ggaatttcat ccctgaatat
     3841 ctccacgatc ctgctgatca tcctgctgtc gttgataacc cagaaatggt gcccgaatcc
     3901 gtcgtcagca acaaaatcat attcaggttt gttctcctta acatattttc cgatcatgtc
     3961 atccaactcc ttatgcgagg ggtatgagaa aaataccggt tctacattgg catcggtgat
     4021 gcggacgtga atcatgcggt cgtcttcctt atccttacgg gtaagttcat gctttttaat
     4081 gacattgttg aaataatcat caatatggca gcagcctact atgccgtatt gtgtccggcc
     4141 ttccatcgtc tgggcataga tgtacagttt ttcttcgtta tcctgtacaa gccagccttc
     4201 tttctggaac cggtcaaaat tcaccactac cttatcatat accgcctggc tgtgctcatc
     4261 tataccgggc tcaaggtcta tttcggcttt ggtaacatgc aacaatgagt agggatttcc
     4321 ttcggcttca atccttgctt cgctggagtt gagaacatca tagggcctcg aagccagttt
     4381 ttttacaatt tcctttggtg gcctcaggcc tttgaatgct tttaaaatag ccatagcagt
     4441 ttctgttagt tatatagttc gttaaaagaa ttttacagtt tttgcaggtc tttcaaaccg
     4501 tttggcaaat ttataaaaca cttcccactt attccctgaa aaaaaaagcc ctgctgaaac
     4561 gttgtcagca aggcaggagt gttgcatcaa taaatgctga ggatgccgga tcaatcgtca
     4621 tcggtatcgg tgatcccggc cgaacgctgc aacgatccga taagggcgcc cagcattttt
     4681 gtcatttcca ttaactggtt ccgtgattct tcttcttcgg tgtgcgaaag gaatccttgt
     4741 ttactggcca tggccgtgtg gacgacacat tccctgacag aactttttgc catcttcagg
     4801 tagtaaataa actggctttt attccgggcc gaaccttcgg ctatgtgcaa cgcaatagcc
     4861 tgggaggaat cgaagaacct gcggctcatg gtcgaggaac cgtcttcgga gaatttgttc
     4921 actttcctgt aaacccaatc aatgtagtcg agcgatttgt ggtacactct caggtcttca
     4981 aaacgaaaga atgtagttgt tttttcaagt tccgaatcca tagtaaaagc ttttgtggtt
     5041 agttggtggt tagttctggt tagtatttca taaataatga aaacatgatg aaaatcttag
     5101 ctaatataat cagagatagg atacaaaaac aaaaatgttg cctgatattt ttctcattat
     5161 cgtgaaaaat cctctgaaga cttttcggct gctgattttt tccaacagca ttaactaata
     5221 atgattaaaa tttcaggcaa ctaatttgaa tgcaaaaaca aggtgtgtaa ttgattttca
     5281 ggttgttact ttttatttac ctgaaaagta gtatcacccg tagcgaaaaa tgaatttatc
     5341 tggcgtgctg cagccaggcc ggcattgttg ttggcttcgg ctgtctgtgc ccccattttc
     5401 tttggagtaa agaagaagcg ccctgtatat ttttcagcaa aatccgattt gcaatcgggc
     5461 tctacatccg acatatactt aaaatcggga cgttgggcaa atacctgcag caatccttct
     5521 tcgtcgataa cttccttgcg cgccgtattg ataagggttg ccccttttgg catttttgaa
     5581 agcagttcga acccgatgga ttttttcgtc tgcggtgttg cgggtatgtg cagtgaaata
     5641 tactggcact ggctgtataa ctcattcaca ttgtcaaccg ggataatgcc gtcgctgatc
     5701 atcgcctttt tatcaacaaa aggatcgaag gcatacactt ccattgagaa tcctttggca
     5761 atagcggcaa ctgctttgct gacgtggccg taagcatgca atccgagttt ttttccttta
     5821 agctcggtgc cggaagttcc gttgaaatta ttgcgcgcgg cataaaccat catggcaaca
     5881 gccagttcgg caacggcatt tgaattttgt cccggcgtgt tcatggcaac tataccagct
     5941 cccgatgcag cttccaggtc aatattatcg tacccggctc cggccctaac gacaattttc
     6001 aggtttattg aggcagcaat tacctcttta tcaacaatgt cgctccggat tatcatggca
     6061 tcggcgcggg cagccgcagc tatcagttcg gctttggtag tatagttttc gagcagtatc
     6121 atctcgtgac cttcaccttc gatgatgcct tttatgcctt cgacggcggc tttggcgaag
     6181 ggcttttcgg tagcaaccaa tacttttttc atgatttgtt tatttttatg aaaaagcagc
     6241 tatcccggca attgccttgc caggataact gcctttctgt taattattta cgcgttttcc
     6301 ttttcaaaat catgcatgca gtcgatcagc gcctgaacgc tttcaaccgg taatgcattg
     6361 tagcaagatg ccctgaaacc gcctacagaa cgatggcctt ttatacctac catgccgtgc
     6421 gctttcgcaa attccatgaa tgcgtcttcc ttgtccttgt actgttcttt cattacaaag
     6481 caaatattca tgatggaacg cgaatccttt tcaactgtgc caacaaacat tttgctgctg
     6541 tcgatggcat cgtacaacaa ttttgccttt gccttatcga gttcttccat cttctttacg
     6601 ccaccaatac tcttgagcca tttgagcgtt tccttcaggg taaagatgtt aacgcaaggc
     6661 ggcgtgttga acatagaacc gtctttaata tgagtgcggt aatcgagcat ggttgggatt
     6721 gcccggtcaa ctttgccaag catatcttcg cggacgatca cgaaagttac accggcagga
     6781 cctgcatttt tctgggcgcc gccatagatc atggcatatt ttgagatgtc aacaggccgg
     6841 ctgagtatat cgctcgacat atcggcgatc aaaggaacgg ggctgttgaa atcttcccgg
     6901 atttcggttc cgtaaatggt gttgtttgtt gtgatgtgga agtaatcaac atccgtcgga
     6961 atcgaccatc ctttgggaat atagctgaag tttttatcgg atgaagatgc aaccgttaca
     7021 acttcgccga ataatttggc ttcttttaaa gccttcttag cccacacgcc tgtttcgagg
     7081 tatgcggctt ttttatttag gaaattataa ggcaccatgc agaattgaag gctggcgccg
     7141 ccgcccagga agagtacctg ataaccttcg gggattgcca gaacctcctt aaacagagct
     7201 accgcttcat cgatcacagc ctggaaatcc ttgctacggt gagagatttc aagtatcgac
     7261 atgccaatac cattgaaatc cagaacagcg ttggccgtgt tttcaacggc gatgcgcggc
     7321 aaaatgcagg ggccggcatt gaaattatgt tttttcatcc tgaaaaaatt tagtgaatat
     7381 attctgattt aaatcattaa gcaacgtttg gtgacgattt ccttaaacac cgtttctgtt
     7441 acaaaagtag aaaaaggctt ccggcattca aagataatgc agaaaaaatc atttcggcgg
     7501 aaacaggttg taattgaccg gtttatgttc actggcattt ttacctgtgg gagggtttcc
     7561 gacttccacc ccttcggcaa atgaccattt acctttatta aagaaatagg catcatagct
     7621 gtcgcccgac ggcacataat actcatattg cccttcgagc tgcggcgtca tcggaacaag
     7681 gtgatcggtc actatcatcc acatactctt ccttttcacg cctttccccc tgggcaacaa
     7741 caaggtctga taatcataac gcaaaaccag cgaagcgttt tcggcatatt tgatgatcac
     7801 cctcggcaca acacctttcg gagtatgaaa aacaggcatg ccgaacaccg gagagccatc
     7861 aggggcaaaa ctcaatacct caatcagttt gctgttcatg ttatcatcaa tcccatccca
     7921 gcccagcagc aaataaatgt tttttttacc gctttttgac gggattattt tataatacag
     7981 cgccccatac caatgttccg gagtggaaaa atcagcattt gccaggtttt gtttaccggg
     8041 aacatcggta agcggaaata ccgcaggatg ttccccaccg gttaccaaaa gcccgtcata
     8101 ggagaatttt ccatccgacg aacggcagtt ccaggtgaat atcctgaacc gcttgtcttc
     8161 ggaggtaatg cggcctatct ctttcagaga atcgaacgga taatcaaaac ttcccggtaa
     8221 ttccatcacc tgccgcaact gttcggcaaa ctttttactg tatgccttca cgtgctggtc
     8281 gtctttctgt tgccatattc tgatgctcat ctttgccagt gagtcttcca gcgcagcaaa
     8341 atcggcctgc tgcccgtaac tgactaacga taaggcaata aaagccaata caaggaaggt
     8401 attaaaaagg ctgtttttca taaccggaaa tcctgaaaga tgcattaacg gaaatacaaa
     8461 cggaattaaa cgcaaactta gtatttacct gcagatatcg caacattcgc cttaattttg
     8521 catccagggt ttgccgaagc aagccttcac cggaggacac gcaagatgat tacacttgag
     8581 aaccgcatca gggcttttat aaaattagga aatctgatgg gacaggcgtc tgcaaacctg
     8641 aagcctgcag caaatgaacc ctgttatacg gcttttgaaa acctgataaa ggccatgaaa
     8701 caggcgtata tccaaaatcc ctggttcacg cccgataaca ttgccgcatc cttcagggct
     8761 tggtcatatt ccctgtctga acaaaaaata agacaatggc tgaatccata tgtaaccctg
     8821 ctggctgatg atcctgagcc caggaaaaca gccgtgatca tggcaggaaa tattcccctc
     8881 gcgggattac atgatttaat ctgtgtcctg atatccggga acagtttact gggaaaggtt
     8941 tcagccgatg acgcctgcct gcttcctgcc attgccggcc tgttatgcgc cattgaaccc
     9001 ggtttcggcc cccttattga gttttctacg gaaaaaatgt cgggttttga catggttata
     9061 gcaaccggca gcaataatac ttccagatat ttcgaacaat attttgccgc ctatccccat
     9121 atcatccgtc acaaccgaaa tgggattgcc gtccttaacg gacatgaaac tccggccgaa
     9181 cttgaaaacc tgggccacga tattttcagc tatttcggat taggctgccg gagcatgtca
     9241 atgatgtatg ttccggagaa ttatgatttc tctctttttt cgggagctgt agctgtttac
     9301 cgtgaccgga tgatcatgca tcataagtac atgaacaatt atacttaccg gaaaacaatt
     9361 tttatgctga acaatacacc gtttatagat ggcggttttt tcctggcatg cgaaaatcag
     9421 gactttatct cacccatatc ggtgatccac tatttccact ataagagtct gccggcgctg
     9481 gctgaatatc ttgaaaatga acatgataac atccagtgca tcctgagcaa catttccaca
     9541 cttcagcatg ctgttccgct ggggaaaggc cagcagccgg aactctggga ttgggccgac
     9601 ggaaccgaca ccctgaaatt tttactggaa aatatgcctc ctttcgaaaa aaatgaacaa
     9661 ggcactgtta aataaatctt tcatactgtt tgctcgaatt gaaaatatca ttacttttgc
     9721 accctcgaaa acaaagcctt cattatcatg aaaaaagaca ttcaccctaa agaataccgg
     9781 ctggtagttt ttaaagatat atcgaacgat tatgctttca tgacgcgttc aaccgtagcc
     9841 accaaggaaa ccatcaagtg ggaagacgga aaagaatatc cgcttgtaaa actcgaaatc
     9901 tcaaacactt cgcatccttt ttataccggt aaaatcaagc tggttgacac cgccggtcgc
     9961 gttgataaat tccgtcagaa atacgagaaa taccagaaac tgcgtaaaca gtcagaacag
    10021 taagactggc ataatgttat tgaaaaggct gtccgctctg gcgaacggcc ttttcttgtt
    10081 atctgctcag gtattttttt aattttatgt caggatagag gccaatgaac aaatttgccg
    10141 gatacctgtt agcaggaata tcgccagcct gagtactggc acaataattg acatggcagc
    10201 atcatcctgt accaattcac cattctgaca tcctgtcacc ccgactgaaa atgaaagcaa
    10261 acaaagacta ccctatctta ttatcaaaca tcctggacga cgaaacggaa tttattccgc
    10321 tcctgtcagc cgaagatgaa gaacatatca atgccgaaag cgtaccggaa gttctgccga
    10381 tattgccgtt gcgcaacacg gttttatttc ccggggtagt aattcctatt accgtcggac
    10441 gcgataaatc gatcaaactt atcaaagagc attataaagg tgaacggatc gtcggcgtcg
    10501 tcgctcaaaa agatcctgat gtggaggacc ctggttttga tgatcttcac ggtatcggaa
    10561 cggtagccta tatcatcaag atactgcaga tgcccgacgg tagtacgacc gttattatcc
    10621 aggggaagaa aaggtttgaa ctcggggaac tggtgcagtc ggaaccttat tttaaagcat
    10681 ctgtaaaagc atacggggct tcgccggaag gcaagaaaaa gaaagacaag acttttgagg
    10741 ccctgatgtc ctccatcaaa gatctggcaa cccaggtagt tcagcagtcg cccaacctgc
    10801 cctcagaagc cggctttgcc attaaaaaca ttgaaagccc tgttttcctt gtaaattttg
    10861 ttgcttccaa tctcagcatc gatctttcgg aaaaacaaaa gctgctggcc attcctgatt
    10921 tcaacgaaca agccaatacc gtgctcggat tgctgacccg tgagatccag atgctcgaac
    10981 tgaaaaacca gattcagaat aaagtgaaga ttgacatgga caagcagcag cgcgattatc
    11041 tgctgaacca gcagttgcgc ctgatccagg aagaactcgg caacaatcct cacgagcagc
    11101 agattaacga attaaagacg aaagcctcca ataaaaaatg gcccgaaaat gtctcccagg
    11161 tattcgaaaa ggaaattgcc aagctgagcc gcatgaaccc tgcggctatg gaatattcca
    11221 tccagatgaa ttaccttgaa gtgatgaccg atttgccctg ggatgaagtc acagacgata
    11281 atttcgatct gaaaaatgcg caacgcgtgc ttgaccggga tcatttcggc atggagaaaa
    11341 ttaaagaacg tatcatagag tacctggccg tgctgaagct aaagaggaac atgaaatctc
    11401 ccatcctctg cctggtcggg cctccgggcg ttgggaaaac atcgctgggt aaaagtattg
    11461 cacgggcgct gggacgtaat tatatccgga tatcgctggg cggactgcat gatgaagctg
    11521 aaatccgcgg tcaccgccgt acctatatcg gagccatgcc gggacgaatc atccagagta
    11581 tccggaaggc aaaatcatcc aatcccgttt tcgttcttga cgaaattgat aaagttgccg
    11641 gcatgaccgt aaacggcgac ccgtcggctg ccctgctcga agtgcttgat cccgaacaga
    11701 actcggcatt ctacgataac tttatggagg ttgaatacga cctttcgaaa gtgatgttta
    11761 ttgccaccgc caacacgatg agcaccatcc accctgccct gcgcgaccgt atggaagtcc
    11821 tcgacctgag cggttacctg ctcgaagaaa agatagaaat cgccaaacgg caccttgtgc
    11881 ccaaacaact gtcggaacat ggcctgactt caaagcaggt caccttcaca aaaccggcat
    11941 tacagaaaat tatcgaggat tatacccgcg aatcaggcgt caggaacctt gagaaagtca
    12001 ttgccaaact tatccgcaac agggcccggt atgtagccat gaatgaaaaa attcctgcat
    12061 cggttactcc tgccgatatt gaaaagatac tcggatcgcc tcacttccag caggaaaaaa
    12121 gcctttcaaa taacattgcc ggagttgtta ccggccttgc ttggactgcc accggaggtg
    12181 aaatcctgtt tattgaagtc agcctcagcg ccggtaaagg aaacctgacc cttaccggaa
    12241 acctgggcga tgtaatgaaa gaatcggcta cgctggcata cgaatacctg aaagcccatt
    12301 ccgatctcct ggatattgac ccgggcgtat ttgaaaaatg gaatgttcat atccatgtac
    12361 cggaaggcgc tacgccaaag gacgggccat cggctgggat caccatgttt acggcgcttg
    12421 cttctgcatt cagccagcgc aaagtaagga gcaaaacagc tatgacgggt gaaatcaccc
    12481 tgcggggaag agtgctgccg gttggcggga tcaaggaaaa aattcttgcc gccaagcggg
    12541 ctaaaatcac cgatatcatc ctttcggaag ataaccgcaa agatattctt gagattaatc
    12601 caatctatat caaaggatta acattccatt acatcagcga gatgattgaa gcgacaaacc
    12661 tcgccctgct caatgaaaaa gtcggccatc ctctctcact gagctgagaa tgaccggctt
    12721 aaacatgact tccggcttct acttcgaaac taccagcttt accgaatgaa ccgaatcgac
    12781 ggtctgaagc ctgcatgtat aaatgccgga tgctaaaccg gatgtttgca ggttaaaggt
    12841 atgatctccc gggttcagat aaccgtccat catcgaagcg actgcttttc cggccatatc
    12901 aaaaagcatc agcttagcgt cggaggcttt ctcaatatgg aaggcaatgg ttgtttggct
    12961 gccagccggg ttcggtgaaa tgctttcgat ataactctta ttttcggcta ccagcggctc
    13021 atctgtcgaa acgatggtac tccctttttt caggactatc tggttgtttg ggtcaaagaa
    13081 aacagttctc ggttctttgt ccgtaaaatc gaacgtaaaa atctggtcgt tgtaagagtt
    13141 aaaaaccttg acaagggtat ccgactggtc cttgaaggtt atccttaatt caaccggcat
    13201 ctgccagtaa atgggcgccg ctgcctgacg acaggtaaaa gttaccctcc agccgttgcc
    13261 gtatggtttg atatcataga cattctgata cttgggatgg ccgccggtat aaatccattc
    13321 atcaaagaaa taatccagtt cctgtcccga aaccgattcg attttttctt tgaaatcgta
    13381 aatggtagcc gatttgtact taaaatcaac cgtgtcggtg gcataagcct ttatagcggg
    13441 gaaaaatgcg ctgtcgccca taacatacct taactgatgc aggacgcaac ttcctttcat
    13501 ataggtaaca ccataatcaa aaaggatgtc gaccgaaggt gttgtcacag cccatgccgg
    13561 atcggagata ggccagggac tgccataggc gaaataggta ctggcattat tcttaatctc
    13621 gtttttataa gcggtatagc cgtctgtatt ttcgatccaa tgggcctcgg accaggtggc
    13681 aaacccttcg ttgagccaga tatcagccca ggtagcgcag gtaatcatat cgccaaacca
    13741 ctgatgggca aattcatggg cgacgagcga ttcggtccag caattgggac aaaggctcgt
    13801 caacgtttga ttttccatac cgccccaggt gaaatcactg ttcagggtgg caaaaccatt
    13861 tttctcaaaa ggatggtcgc catattcctg cgaatagaaa gttaccagat cggggaaaat
    13921 ggattccatg ccatccgggt tttcaccggg gttggaataa aaacgcatcg gggtcacttc
    13981 gcctgtattc ggattggtcc agttaacaat atccaggtta taaccagcgc gtgaagtaag
    14041 aatcgtcagg taggtggcca ccggatcgcg gcttatccaa tgataggtca ggatgttgcc
    14101 attcacagta gagtcctgca acctcccgtt cgatgccagt tttacattca gcggtaccct
    14161 ggcggtaaga tctgtggtgg ctttatccga agggtggtca taacagggaa accatttccg
    14221 ggcgccttca ggttcacaat cagtgaaaag gaatccggaa ttcacataaa acgcgccatc
    14281 cgaaacattt ttatgatgat aactcacttt taccgctgcc tgttcaccgg gattataagt
    14341 tcggtcaagg acaatgttca gcaaatccga agcatgggta aacgacaccc cgttcatacc
    14401 gaccgaatct atctccagtg aagtattgac tgcattcagg acgatgtggt cgatcaccga
    14461 atcagcttca aaggtcagga tgacactacc ggtaaaactc ttcgggtacg gcgaaatgta
    14521 acaattgtaa agatcaaggt cgaatttata attcagcaca tcgaaactat gcaccggcgc
    14581 attcggtgat aacatcctaa gatcgggcag ttgctggatg tgcgatttgc ggtacgaaca
    14641 caactgggaa ccgctcatgg ttgagaaatc ctgtgccgac agaattccgt aaagtccgag
    14701 aaaaagaaag aggatggtaa attttttcat attgactatt tttcagccgc aaatgtaatt
    14761 tatttgcaat gatccttcat gaaaacttaa tgcactattt ttgaaaacca aattcccctt
    14821 aaacaagcga tgttccgaaa aacggccatt atactgatat tatcgctgtt atgctttgcc
    14881 tgccaccgcc atcggcccgg cgatgccgga aaaacagtct tcaggtataa cgaatcagcc
    14941 ggcataacct cgctcgaccc cgcttttgcc agcaaccagg ccaatatttg ggctgtaaac
    15001 cagttgtata acggactggt tcagcttgat gacgccctga aagtcaggcc ctgcattgca
    15061 aaaagatggg aaatttcgaa tgacgggctt atctatacct tttatttacg caacgatgtt
    15121 ttcttccagg attcgcccat ttttaaaggc gggaaagggc gaaaagtcgt tgcctccgac
    15181 tttgtttaca gttttcgcag gattgccgat ccaaaaatcg catccccggg agcctgggtt
    15241 tttggccagg taaaatcagc aaacggacta cctgaattcc gggccgttga cgatacaaca
    15301 ctccgggttt tcctgagcgc ccccttccct ccgtttctcg gcttattgac agttcaatac
    15361 tgttctgtcg ttccccatga agcggttgaa cagtatggcg ctgatttcag gcgctcaccg
    15421 gtcggcacag gacccttccg attgaaaatg tggaaagaag gcgtgaaact tgtcctggtt
    15481 aaaaacgaaa actatttcga aacagaagga aagcgccgcc tgccttttct tgatgcaata
    15541 gctgtcacgt tcatcgtgga taaacaatcg gcgttcctcg aatttgttaa aggcaacctc
    15601 gacttcatgt cggggattga tgcgagttat aaggacgaat tgttaacgcg tgacggggac
    15661 cttaatccaa aatacaggaa taagatttcc ctggttacga gtccttacct gaacactgag
    15721 tatctgggca tacttgtcga taccggcaac aagctggtca ggaccacccc gctgcgcctg
    15781 ttaaagatca ggcaggcgct caactatgct ttcgaccgca aaaagatgat gcgatacctg
    15841 cgcaacaata tcgggatacc gggagttgcc ggaatggtgc cgccgggtct tcctttcttc
    15901 gaccaggcaa aggtatacgg ctatgattat aatccggaac gcgcccggca attactggcc
    15961 gaagccggtt tccccggagg aaaaggtctg ccgtcgatca cccttactac caacgcgtcg
    16021 tatcttgacc tgtgtaaata catccagagc cggttgaatg aaacgggatt cgacataaaa
    16081 atcgacgtta cgccgccggg aaccttgcgc gatatgatag ccctttcgaa agtaaacttc
    16141 ttcaggggat cctggatcgc cgattatcct gatgccgaaa actacctctc actgttctat
    16201 tcccctaact tttgtcccga aggaccaaac tacacccatt ttgccgacaa ggattttgac
    16261 cttctgtatc gcagggccgc ggcggaagcc aacgattcaa taaggggcag actatacatg
    16321 gaaatggaca acctcgtgat gcggcaggcg cctgttattg tcctgtatta tgaccaggtg
    16381 ttgcggttta cgcaaaaaaa tattcatgga ttgactccca atccgctgaa tttacttacc
    16441 ttgaaacacg tcaggaaaac gaaataatat gctaagacaa tctcctgtta tcgatgaatt
    16501 aaagaagctg gacaaaagtg tattttccat gcttgatgac gactggatgc ttattactgc
    16561 cggcaattta tcggcgttta acaccatgac cgccagttgg ggcggtttcg gggtactctg
    16621 gaacaagccc gtggcttatg ttttcgtgag gccaacgcgc tttactttcg gctttatgga
    16681 gcaatcggaa ctattcacac tctctttttt cgaagaaaag taccggcata tcctgcaata
    16741 ctgcggaaag gtttcgggac gcgataccga taaaattgcc ggggccggcc tcacaccttt
    16801 aaaatcaccg tcaggcgccg tgatgttcaa acaatcaagg ctcgttttcg attgcaggaa
    16861 aatttacact tccgatatca atccttcaga ttttcttgat cctgccattg agaaaaacta
    16921 cccggaccat gattaccatc ggatgtatat cggggaaata accgcttacc tgactgacca
    16981 ggatatttcc gggacgccgg gctggtaaaa gtaatttgct cacctgatca ggatttttta
    17041 atttagatgt tcttccgaca aaaaaaccac caataaaata ttaatttcgc atcttccaat
    17101 tcaaacgaat cttgggattc ttaaacaaca tacgtcagaa actcgcctgt cgccgcctga
    17161 accaggaaca ccaaaaactg gcccggatca ggaaatctac cagcctggag aatgcccggg
    17221 ctattggcat cctttactac ctccctgatg aggacaccta caaacaggta gaagagttca
    17281 tcaggttatt gaccgatatg aacctgaaag tccgtgtagc ctgctatacc gacaataaaa
    17341 ttccgcctca ttactttatt ccgaaactgt tgcaggatat aataacccca aaggatttaa
    17401 gctggattgg cgtacctgaa aaactatttg ttcccgactt tttagccgag aaatttgaca
    17461 tacttattga cctcagtctt accgatcact tcccgctgct atatcttgcc gcgttatcca
    17521 aagcctcgtt gaaaatcgga cgttacgatg ccagccgaca ggagttcttc gatatgatga
    17581 tccacacatc gcctgataca tcgcttcagg aattcacaga acagatttta cattatctga
    17641 aaatgttcaa caataccaat aaagcatgaa ttccaaattt aaaggcacag gggtagccct
    17701 tgtgaccccg tttcacaaac agggaactat tgatttcagt tccttaggaa aacttattga
    17761 gcataccatc gaaggcggag tgaattacct tgtggttcaa ggcactactg ccgaaacggc
    17821 tacactcact aaggaagaga aacatgcggt atcgcagttc gtcatcgata cggttgacaa
    17881 acgggtgccg ctgatgctgg gcgttggatg caacaacacc caggaagtgg taaatacgct
    17941 gcgtaccgct tcctacgaag gtttcgatgc tgttctttcg gtgacgcctt attataataa
    18001 gccccagcaa agaggccttt atcttcatta caagaacatc gccgctgtaa gcccgttgcc
    18061 catcatcatg tacaacgtcc ccggcaggac cagcgttaat atgaaagcgg agacgaccct
    18121 tgaactggcc cacgaattcg acaacatcat cggcgttaaa gaagcatccg gaaacatcga
    18181 acaagtcatg gatattatcc ggcagaaacc gaaagacttc ctggtaattt cgggtgacga
    18241 cctgcttact gcccccatgc tgactatggg cgccgacgga ttgatttcgg ttgccgccaa
    18301 tgcattccca aaggaaattt cggacctggt gatgtacggt atgaaaggcg atctgaagaa
    18361 gatgcgcgaa attcattacc ggatgacgga ttttatcaaa gccattttcg ctgacggcaa
    18421 tccgagcggg ataaaagccg ccctggaaat aaaaggcctc atttccaata acctccggct
    18481 gccgctcgta aaaatcgaaa agaatcttta taatactctc gccaccctga ttcaggaaat
    18541 agaacccgga aacttgtcat aactcagaaa acatcttttt cccaacaatg aagaaaataa
    18601 tcctttttgc ctgtattaca ctttgtgtaa cggttaacgt atgggctcag caaaacgcgg
    18661 cagaaatcag caaggcttac gaggccatta atgcccgcgg cgaagtgtat ttcagcttta
    18721 acatatccga ccgtaatgaa atcggagccc ttacgaaaat catatccatt gatgatgtta
    18781 aagggaatac cgtttatgca tacgccaatt cgaaagaatt taatgctttc aggcaactgg
    18841 gctacgattt caaagcgctt accgcccctt cattaatgtt tgaagccgcc atgagtaaca
    18901 atccggacca ggttctggga tgggattatt atcctacata ctccgcctat gaaagcatca
    18961 tgtcgcaatt tgctgccgat caccctgacc ttgcccggct tataaccata ggaaccctcc
    19021 ccagcggacg gaaacttctg gctattaaaa taagcgacaa cccggacgtt cccgaaaacg
    19081 aacctgaatt cctttataca tccagcatac atggcgatga aaccacaggg tatcccatga
    19141 tgctgcatct catcgactac ctgctgacca attacggaac cgatgaccgc atcaccgatt
    19201 ttgtcaataa catggaaatt tacatctgcc ccctggccaa ccccgacggc acatatcatg
    19261 gcggaaacac ttcggttaac ggcgctataa ggtataatgc caataatgta gacctcaacc
    19321 gcaattatcc tgatccagag ccccttgatc cgggtaatcc caattacgac catatgcatc
    19381 ccgatggtct cgcttggcaa aatgaaactg ttgcctttat ggattttgct tcccagcatc
    19441 attttacaat ggcagccaat tttcacggcg gcgtcgaagt cgtcaactat ccctgggata
    19501 cctgggccaa attaacagcc gacaacaact ggtgggttta tgtaagccgg gaatatgccg
    19561 ataccgtgca cgtcaatgct cccaacggtt atatgaccta tttgaacaac ggcatcacca
    19621 acggttatgc atggtacagg atcaccggcg gccggcagga ttacatgaat tatttcaaac
    19681 attgccgtga agtcaccatt gagatttcgg acaccaaact tgtgcctgcc agccagttga
    19741 tcccgcactg gaactataat taccattcac ttctgaattt catgaacgag tcgctgtatg
    19801 gcttacacgg aatagtcacc gatacggtaa ccggcctgcc cctggaggct aaaatattta
    19861 tcaccaactt cgataaggac tcctcgcatg tgtataccga tccggcagtg ggcgattatc
    19921 accgtttgct gaaagccgga acctataacg tgaccttctc tgctccggga tatatctcta
    19981 aaacgtatca ggtgcaaatc gccgacatgc aaaaaaagac gctcgacgtt cagttattcg
    20041 acggcgccct tacagcgtca ttttcggccg ataccaccaa tatttacccg ggaacagaag
    20101 ttcatttcac cgatcacacc ctcggatatc ctgccagttg gcaatgggaa tttgaaggag
    20161 gaacgccggc aacttcaacg caacagaatc cggtaatcac ctacaataat tacggcagtt
    20221 ttaatgtaaa acttatcgtc agccgtaccg atggttccga taccctgctc atgcagaatt
    20281 acatttcggt tgcaccgagc taccttcagg gtaactatta tttcaccacc tgtcacggga
    20341 attactttga ttcgggcagg gaaaacagca attattccga taatgaatta tcggccatta
    20401 cttttgttcc cggcgagtca gataaaaaga tcaccattta ctttgttgac tttgatgttg
    20461 agcaaagtgc aaactgcgat aacgaccgct tgactattta cgatggacca agcactaccg
    20521 accccgtaat aggcaattac tgcggcacat ccatccccga atcggttacg gccactaatg
    20581 ccggcggcgc ccttacggtt ttattccgtt caaattcatc ggtaacagga gccggatgga
    20641 atgcctatgt tgtttgttcc cccaataccg gcatagccga agatgaagac gcttccataa
    20701 agctgtttcc aaatcccgca ctcaacggga gcgctaccct tatctcaccg gcaatcataa
    20761 attccttgca gataaccgac tgcctcggca aagttgttta caggctcaat cccggcgcca
    20821 ataatgtgca gataccttcc ggtcttgcag ccggcctgta tttcgtagca gtgcaaaccg
    20881 gagagcaatg gtatgtaaag aaattacaga taatgcggta agcaaaaatc ttgaactgaa
    20941 aaaagcaggc gtccggcctg cttttttatt tctgaaacag ccgaactata tcaggaaccg
    21001 gatataatcg acaagcaatg gcaattcctc cgatttcacg ccgtttgctt cgagcaccgg
    21061 ggtatcgttc tgtatcactt ttgcaaatct ggcaacgctc atgccgggct gcctcaaagc
    21121 ctgcatgtaa tactccatgg ctttctgttt aaaggcaagg cagtattcca catgcccggc
    21181 attgatatag tcgttggctg tcggctccga ttcaaggatg gtttggagat agttgcgggc
    21241 gatttcaagc tttccgctta caaacgagca ccaggctata gggcggagca cctttttgtt
    21301 atccggcgcc agaaattcca ctttaaaata atgttccagc gcttcctggt agtttttcag
    21361 gtcgaagagg caattgccgg ccatcgtatt gatataaaca tcatcagggt caatcgacag
    21421 ggcttcgttg caccatttca gcgcttcttc atatttattg agttgcctga ggcaaaaaat
    21481 gatcttttta acgctccaca gccggtttga atcgaacatt tcgctttgac ggtaaaaacc
    21541 gagcgctttc tcatagtcgc cgagccgctg atatccgtat gctattttgc cgatcagggc
    21601 gcctgaaggt tccggttgcg aaaccaggtg atttaataac gggatagctt cgttgtaacg
    21661 ttccttttcg aaaagaaatt cggcaaggtc gtttaccagc gcgtggttcg atttgattat
    21721 ttcaaacaaa gcgctgccgg caatatccca tttcatggca aaaatatccc tgaaatcgga
    21781 tcggaacgga tgaagtttgt aaaacctgta caaatcctga acgtactggg taaaaatgta
    21841 actttcccgt cgcggattgg ccagcaggtt ttcttcgtct ttcagttcac ggaggctttc
    21901 cgcttccatg ttaaacaatc cgatgattcc gtttcgctgg tttgccggaa ggtgactgat
    21961 gttgaaacag aaggaatact tatcggagtt gcacatgtaa aatgaattct ccatcccctc
    22021 aaggaaagca cccccctttt cgccttcgcc ggtcacagat gatcgcacag cttcgttttc
    22081 cttgtaaaaa ggcctgaacc agttcgaaat atcgcggaag aagtcgaaat gcttcagcat
    22141 ggcaaaggtc cccatgaaca agtcggaacc ttccatctgc atgttcgaca ttttttcaat
    22201 cttatcgagc aatccgggcg aatcttcgaa cagcgtctcc cagtcaggat ttttatcctc
    22261 gataatatcg ctttccagga tgttgtcaat gtcaagcttt tcgcggattt tctcttctat
    22321 tttctggata tcaggactta tatcttcctg aaatttgcgg gtgactttct cggtttcaag
    22381 cgctttaaag agggcaatca gcacctgtcc catatcacga taaaactgtt catcttcctg
    22441 atattccagg attttcgcac tgatagccgg gtaaaaaccg atgcggcggt cgtaataata
    22501 gatggcaatt acaattgccg aaagcgcccg ctgccatact tcattctgac gctgttcata
    22561 aaatttgaac aacagcagaa atttttcagt gtcataacaa tttaacaggc tgagagtaat
    22621 cgcactcaca accaggcatt tttcatacca cggcagttct tccgaaaccc ggatagcctc
    22681 tataagccgg atatcggtat cggtatattt atccgacagc cagatcatct ggaagagctt
    22741 gggagtaatt tcatgcagac gaccctttgc cgtttcaccc tgcgcctgaa gttctgtttc
    22801 ggagagcacg cgctccagtt ccttgtcgaa tgagagcgtg tcaaaattcc gggcagcatc
    22861 ttcttttgcc agttcctttt ccttttcgag ctgcatcttc agggaatagg tgttcatgcc
    22921 cgtcatttca aacgcttttt gctgcaccat gtcagccagt tcgaggattg aaatcttgag
    22981 cgaacgatat atcctgtcgc ggtgaggatc cggaacgccc ctgaaggtat attcgagcaa
    23041 catgcggtag gtctggtcca gattttcgta ctgcatggta aaatcagcca catgcagtcc
    23101 ttccatcatc ctgcccatcc gcgatagcgc ttctttcagc ctgttgtcga aaaccagggc
    23161 acaggtttcg ttgaaaaggt tggtgatctc agctttttga ggcataatac cagcgggtta
    23221 atcctgatta ttgtatcata cgcatcagtt catcttcaga gatgatcggg atgttcaatt
    23281 gggctgcctt tttctgtttc tcagggccca tgttctcacc ggccagaacg aaagtggtgt
    23341 ttttcgacac cgatcctgca tttttcccgc catgggcttc aatcagagat tttatttcgt
    23401 cacgcgaacg gctgaacacc ccgcttacca caaaaacttt gcctgccagt ttatctgaca
    23461 aattggcgtt cgccgaccgg ctcagctcaa accggatacc ggcggatttt aagcgtgcta
    23521 ttatttcaag gtgcttagga ttgctgaagt aatcctgaat gctcgccgct atcttggtcc
    23581 cgatttcttc cgccgtaagc agttcttcgg ttccggctgc catcagggca tccatggagc
    23641 cgaaatgcct ggccagcttt ttggccaccg tttcgcccac atacctgatt cccagggcat
    23701 aaaccacccg ctcgaagggt acatttttcg aggattcaac gccgttcagg atgttttccg
    23761 ccgatttttc cctgatcgaa accttcttct ctttctggtc cccgtttccg atcaccttgg
    23821 tgatgccaat cagcttttcc ccagtaaggt cgtaaagatc ggcgacatct tttaccaggc
    23881 cgctgtcgta aagcatttcc actttacctt cgcccaggct ttcgatattc atggccttac
    23941 ggctgataaa atgctccagt ttccctttga tctgcggcgg gcatccgtcc tcgttcggac
    24001 agtaccaggc cgcttcgccg ggattgcgga caagagaggt cccgcattca gggcaatggg
    24061 ttataaattc ctgaggtgcc gaaaacaaat cccgctttgc catttcaacg ccgatgatct
    24121 tggggatgat ctcgccgcct ttttccacat aaaccgtatc gcccaccctg acgtccagct
    24181 tttcgatgac atcggcatta tgcaaggtgg cgcgcttcac gatggtaccg gccagcttta
    24241 ccggctgaag gttagccacc ggcgtaacgg ttcccgtccg tcccacctgg taatcgatcg
    24301 aaagcagccg ggtggccgcc ctctcggcct tatatttata ggcaatagcc cagcgcggcg
    24361 acttggcggt aaagccaagc atctgctggt attcggttga gttaaccttg ataacggcgc
    24421 cgtcgatatc ataaggcaat ttttcacgct gtttcccgat ctcgaggata tagttccaca
    24481 cttcattcat atcccggcac accctggtca tgtccgaaat cttgaagccc catgatttca
    24541 gcgccatcag gctttccgaa tgcgtcataa aaggcagtcc ttccgccgca agaaagtagc
    24601 agaaattgtc gagcctgcgc tttgccactt cggaggaatc ctggagtttc aggctgccgg
    24661 ctgtggagtt gcgcggattg gcaaaaggcg gctcaccgat ctcaatacgc tctgcattca
    24721 tcgcatcgaa agcgctgcgg ggcataatga tctcgccacg gacttctatc tcatcgggat
    24781 agccggttcc tttcagccgc aggggaatgc ttctgatagt ccggatgttg gcggttacat
    24841 cgtcgccgcg aaccccatcg ccgcgcgtca aagcccttac cagcaaacct ttttgatagt
    24901 tcaggctgat ggatacgccg tcgaacttca gctcgcagac gtattccgcg ttatcgccga
    24961 cggctttgcg cacccgttgg tcaaaatcgc gcaattcctc ttccgaatag gtattgccca
    25021 gcgacagcat cggtatttta tgctcgtaag actcaaattc tttggtaaca taccctccta
    25081 ccctttgcgt gggtgaatcg ggaagcgcca actccggatt tgctttttcg aggtctgtaa
    25141 gttccctgag cagcatgtcg aagtcaaaat ccgatatttc gggctgagcc tctacataat
    25201 aaagataatt gtgacggtta atctcgtcgg tcaggaattg gatacgttcg gcaacagggt
    25261 tttcggtcat cgttctttct tcttttcggg gaaataagcc ttgtttcggt aaatgtatat
    25321 atcctggttc aggtttaccg ggttaatcca atgcataaac cggtcgacca tgcccgaccg
    25381 gatggttgtt tcatactcca gtccgggaag ataagttctc agcatgttaa cacgcccttc
    25441 gaggctgtcc ttttcaaaaa acaacacgaa acgggcatat ttcaggctat cggcatccgt
    25501 aaaccttcgc ctgaaattca tcctgagccc cgtcttcgat ttttccatca catccgccca
    25561 ctgacccata tagtaaaccg gtaccatctt ggcgctgttg tgcctggaat cttcgagcaa
    25621 tatcatcttt acatctttat atcccgacag gtacgtcatg gcttccaccc tcgatttttt
    25681 cgaataggaa aagctgatgg ccagtagcag aataatgttc actatccaga aaaatttcca
    25741 gcttccgctg atgaagcccc ggtgccgttt caggaatccg gacttctgca tcattgcgct
    25801 ccatcccgct gtccccagaa caatgacgaa aggaatgacc gggaaaataa acctttcctg
    25861 tttgttcgga aaaaccgaat gaaaaacaag gaatacaaat gccggaagga aaaggagcaa
    25921 ctgtttccgc catgaggcaa aaaaaccggt cagcaggaat atcgacaccg gcgggatgag
    25981 gattccggcg agcaacaaaa tataattgta ccaggggcca atgatatagt catttgccgc
    26041 ctggatgtta tagcgcacat attcggcaaa ttcggtaaaa ggcgctcccc agatgaagta
    26101 atcgatgagc ccctgtaccg gtaaaacgga agccagtacg ccgattccga ataccagtgt
    26161 cggcttccaa agcttcagga tgagcatggc caatcctaaa ccggcaatga acgacgccgt
    26221 ttgaaaccgg accgaaaaag ccagtcccat gaccaggccg gcggcaaaat aacgccataa
    26281 gggctttcgg gcatcatacg cgctgatgat gagccaggcg ccatacatca ggaagggaat
    26341 gcaaacgatc tccaccaggt tcctgacgct caggaagggc ataaaccaga agaaagccgt
    26401 gagcaaggct gccgtaaagg cttccttttt ccccgagatc ttttcggtga tccggtaagc
    26461 aaaataaacc gtcagaaggg aaaacaacgc atgaatcacc ctgaccagca gcatcctgac
    26521 ctgcggatcg gcaatgcccg caccattgca cacaacgaaa aacaggtaat gcaatcctga
    26581 ataaaacagg ctgtgcccgt cgggatgcgg atgaaggcca ttcatcggaa gccattcctg
    26641 gtaattaatt ccctgcgacc accagtagga cggttctatc accaggaaat gatcgtcgtg
    26701 catcccgaat cccctggaaa atatcgcgct gagcaaccgc agcaaaaggg ccggcaggag
    26761 aataaaaagc aacggcctgt attcccgcaa acgttttatc tgtttaatag tgagcatcat
    26821 gaaaggcaag tccggatttt ttgttttcag gttaaagtta aggaatttcc ggcttccgca
    26881 tttattgcct tttatgtatc ctgaaaagac gggcgctaag agcaagttgt ttaatttcgc
    26941 atgaaattat attccattac gcatgaaaag aatttttacc ttctgttctc ttgtactgcc
    27001 gcttttcggt ctgttaagcc cggttcttac gttttcacag gaaagataca cccccgaact
    27061 gctctggaaa cttggccgcg tgagcgaagc gcaggtttcg cctgatgccg gccggatcct
    27121 gtacggcgtt acctggtaca acctggaagc caacaaaggc aaccgcgatc tttatatgct
    27181 gccggtcgcc ggcggacggc ctgtaaaagt gacttcgttc caggggagcg aatcgaacgg
    27241 catctttcgc cccgacggca aaaaaatagc tttcctttcg tcggaatcgg gatcgtcgca
    27301 gatatgggaa gccaataccg acgggacaaa tcccgtgaag atatccaatg aagacggcga
    27361 tatcacgggt ttcgcgtatt cgcccgacgg cagccggatc cttttcacca aacgggtaaa
    27421 actggataag tcgcctaacg acatctaccc tgaccttccg aaagccaaag cccgcatcga
    27481 ggacgacctg atgtacaggc actgggattc gtggagcgat tactgttaca gccatatttt
    27541 cgtagccggt tataacaaag gcgtgatcgg caaaccgacc gacattatgc ccggcgagcg
    27601 ttacgattca cccatgcttc cctggggcgg aatggaacag atctcctgga gccccgatgg
    27661 gaatagcatc gcctacgtct gcaagaagat gaccggcctg caatatgccg tcagcacgaa
    27721 ttccgatatt ttcgtttatg atatagcctc cggaaaaacg cgcaatatca gcgaatccaa
    27781 tccgggttac gaccaggatc ccgccttttc gcccgacgga tcaaaaatcg tttggcgcag
    27841 catgcaacgc gaaggttttg aagccgacaa ggaccgcatc atgatcttcg acgtgaagag
    27901 cggaaccatg caggattatt cagccggctt cgaccagagc tcaaagaact tcacctgggc
    27961 cgccgacggc aaatcgctgt atttcatcag cggtcaccac ggcaccgaac agatttatcg
    28021 cctcgacatc gcaacgaaag ccattaaaca aatctcttcc ggcatgcacg attatctttc
    28081 gatcgcttta tcgggaaacg acgtcattgg cataaaacaa agcatgctaa tgccggccga
    28141 aatctataaa atagatgcaa aaggcgccga gacgcagctc accttcacca ataaagagat
    28201 cctgaagaat gtaaaatcgg cttccgttca ggaacgctgg gtcaccacta ccgacggaaa
    28261 aaagatgctg gtatgggtaa ttctgcctcc ggatttcaat cccgccagga aatatcctgc
    28321 cctgctgtat tgcgaaggcg ggccgcagag catggtaagc cagttctttt cgtaccgctg
    28381 gaattttcag atgatggccg ccaacggtta catcgttgtt gcgccaaacc gcaggggact
    28441 tccgggtttc gggcaggaat ggaacgatca gatcagcggc gattacggcg gacaaaacat
    28501 gaaggattac ctcaccgcca ttgacacgct ggcggccgaa ccttacgtgg acaaagaccg
    28561 gctcggggct gtcggcgcca gttacggcgg attttcggtt ttgtggcttg ccggccacca
    28621 cgaaaaaagg ttcaaggcct tcatcgcgca ttgcggcatg ttcaacctcg aagaccagta
    28681 ccttaccacc gaagaaatgt ggttcgtgaa ctgggacctt ggcggaccgt actgggaaaa
    28741 aggaaatccc aaagccgacc attcctatgc caactcgccg caccgctttg tcgaccaatg
    28801 ggacacgccc ctcatggtca ttgaaggcga gaacgactac cgcatcccct tcacccaggg
    28861 catggccgcc tataatgccg ccaaactcag gggcataccg gcaaggtacc tgcacttccc
    28921 cgacgagagc cattgggtgc tgcaccctca aaacagcgta ttgtggcaac gggaattctt
    28981 ccgctggctc gatcagtggc tgaagtaagg aaacaagtta cagggacaac gattccgaca
    29041 tatcaaatac ggaatttcgc aggcaaaaat tcaggggccg gcagcctgat catgggtgaa
    29101 aggcttcagc aaaccgggat gatgccgggc agggcgaccc ttttctttcc ggatgttact
    29161 ccggctgtta cggctgatca ggaagggcta tttatcagtt cgtctatctc ggaaaggtgc
    29221 agcttaagat gcaggggata atcccggatc atctcctcca gggtgatcat cgaatcgggg
    29281 cctgacatcc attcgttttc gagctttcca tgatccacgt tgcggatgac atggcacaga
    29341 tgcaggttgg tatatttcca gagcctgatc aggtcgttcc agtcttcgtt ctggtaatcc
    29401 tggatagcga tccagcggtc gttgttgccg ttggtagcat aattgggaaa cgacagcggg
    29461 ctttgccggt actgcagatg aacgatcctg tgaatgttgt tggaagccga atccacgagg
    29521 tggccgagta tttgtttgat ggtgcggttc tgccggttcc ggcgctgcat gatgattccg
    29581 gccggcagcg atgcaagcac aggctcccag gcttcggtta aggaatgaat ttcgctgatg
    29641 atgacggagg tttcgaacat gacgatggtt ttgagtaagc ttagcgatat ttcaaaaata
    29701 ccaagatctt ctttaaaact tctcagacta aacttaattt cataaaaaac cttacatagt
    29761 ctgcaagagc attgcttcga aagaggtttt taccgggaaa aagacatatg gctcaaccgg
    29821 aaataattga tgggctcccg ttccgggaca gactttacag accgctcata ttcctatttg
    29881 ttaacgccag gcgattattc cttggtcaat gattcaatga tgtttattaa atcctcaaca
    29941 ggtttgaaat agggcattac cttctcttca gtaaaatcaa atagatcatc ataatctcct
    30001 ttttgtctcc aggagaataa ttgtgaatag aacttcccat aatccttgga gataatacct
    30061 gattttataa aatgctcaga gaaattatgc tttgctccat tatgagtggt agggttaaga
    30121 tcgtgtttga gtaataaagc catcaccgca taataggcgg aataatataa cctgttgata
    30181 gctgaattcc atttttcatt attagcaaga atagttgcat catcaagggt ttctttagcc
    30241 ctttttaatc gataacttat taaatcttgc tttgatccgc tcattggatt cttattcctt
    30301 catttttaat attttcaaat aacggggtaa tataatgctt ctcttcccaa tcttttttcg
    30361 aataaatcaa gggagaaatt atttcgccag attcaagttc tatatcatat atctgatcca
    30421 ttagacttgt ttcgagcgca aatgggatat tgttaatatt caaaagaata agaagatccc
    30481 aatcagacaa acgatgaggg tttcctcttg cctgggatcc ataaagatag acttcagctt
    30541 ttggatccgc cgcatttgtt aacctgatta tctcgctcaa tatcttatct ttcttagtca
    30601 taacacaaaa ataagataat ctttaacatt gttgtccggt aaaccggaat aattttaatt
    30661 ttttgttttt aatgctcagt t
//
LOCUS       DAOPYV010000035        29918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_1513_length_29918_cov_12.316479, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000035 DAOPYV010000000
VERSION     DAOPYV010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29918
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1513_length_29918_cov_12.316479"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..2445)
                     /gene="bglX"
                     /locus_tag="PKI35_06265"
     CDS             complement(151..2445)
                     /gene="bglX"
                     /locus_tag="PKI35_06265"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HNX44025.1"
                     /translation="MKRIKALLLGALLLSGISVSAQTKPQAADQKIKVFIDALMKKMT
                     LEEKLGQLNLPDAGQFVTGEMGSSDIGKKIKEGKVGGLFNIKSVEKVREMQKLAIDQS
                     RLKIPLIFGMDVIHGYQTTFPIPLGLAASWDLPLIEQSARIAAIEASADGICWTFSPM
                     ADICRDPRWGRIAEGAGEDPYLGSQVAKAFVKGYQGDDLRKNNTIMSCVKHFALYGAS
                     EAGRDYNTTDMSRIRMYNEYLPPYKAAVDAGAGSVMTSFNEIDGIPATANRWLLTDLL
                     RHQWGFNGFVVTDYTAINEMIDHGMGDLQTVSALALHAGVDMDMVGEGLLTTLGKSLQ
                     EGKVTQTEIDQACRRVLEAKYKLGLFDDPYRYCDDNRAKTEIFTPEHRKIARETAART
                     FVLLKNENQVLPLQAKGKIALIGPLADNRENMTGTWSVASDYSQSVSLLEGLKNAVGD
                     QAEILIARGTNIVNDPQLDARISVFGKPTRFDNRPPQEMIDEAVKIAAASDVVVAALG
                     EAAEMAGESSSRSDISLPENQKDLLKALLSTGKPVVLVLFTGRPLAIPWENENIPAIL
                     NVWFGGTEAGNAIADVLLGKVNPSGKLPVTFPQNAGQIPIYYNHKNTGRPLPEGQWFR
                     KFRSNYLDVSNDPLYPFGYGLSYTHFTYGDLKLSSTQLKGNQTLTASIDLTNDGKYDG
                     AEVVQLYIRDMAGSVTRPVKELKGFRKVFLKAGETQTITFTLSPEDLKFYNYDLKYNW
                     EPGDFEVFIGGSSATELKKTFTLL"
     gene            complement(2633..3958)
                     /locus_tag="PKI35_06270"
     CDS             complement(2633..3958)
                     /locus_tag="PKI35_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNX44026.1"
                     /translation="MSCSFHQKKTAVSGNVTTRPEISDDSLLTLVEYRTFQYFREGAE
                     PVSGLACERIHTDGIYPEHDQSVITSGGSGFGVMAILVGIERGFITREEGYKQLRKSV
                     DWLSKADRFHGAWPHWMYGETGKVKPFGQKDNGADLVETAYLMQGMLCVREYFKNGNA
                     DERKLSSDIDKLWKGVEWNWFTNGGKDVLYWHWSPTYGWAMNFPVEGYNECLILYVMA
                     ASSPTYPVTAGAYKKGWARDGKIKTRSGKYGYELTLRHNGAEEYGGPLFWSQYSFLGL
                     DPRGLKDEYADYWSNNVNHTLINRQWCIENPNHFKGYGENCWGLTASYSTVGYSAHAP
                     GKNTDLGVISPTAALSSFPYTPEYSMQALKHFYFDLGDKIWGKYGFYDAFSEQNNWYP
                     ARYLAIDEGPIVVMIENYRSELLWKLFMQCPEIQAGLKKLGFESPWLRK"
     gene            complement(4053..6020)
                     /locus_tag="PKI35_06275"
     CDS             complement(4053..6020)
                     /locus_tag="PKI35_06275"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNX44027.1"
                     /translation="MKVIPAFLVIIAAGVLLESACKKEQVPEGTLQLIQIKAGDVVFK
                     LQDTTENVPADSRITLEFGSALDTVSAKENILLKKDNSTVSTTSGFSADGKIITLKPV
                     ELLESRKYYTIRISGSLKGAAGETFQTMEFTFITVAGQLILRSITLNGQDFSSHVSLR
                     NIDYKSVNIEVSFSHALDPASYKSYMSLAENPGISCSLSDNDTKVTITNNSALKYLWR
                     YYFNISSALKSKNGLSFSGFSNAFYTGIDSTLKFPLVTDEELLTAIQQQTFRYFYDYA
                     HPACGMARERSGSGDIVTTGGSGFGIMALIVGMERGFISRSEGTTRLGKIISYLETSD
                     RFHGAWPHWSNGNTGAVIPFGTKDDGGDLVETAFMAQGLLTMRQYLNPADADENSLIN
                     RITTLYNGIEWDWYTRGGQNVLYWHWSPNYGWDMNFPIHGYNETLITYILAASSPVHT
                     IPAAAYHQGFALNGGIKNGKTFYGYVLPVGYDYGGPLFFAHYSFLGLDPRNLSDAYAG
                     YWIQNVNHSLINWAYCTDNPKNYPNYNAYCWGLTASDIPSGYGVSEPANDRGVIAPTA
                     AVSSLPYSPEQSMNAIRFFYYILGDRLWGEYGFRDAFDVHSNWWAGSYIAIDQGPEIC
                     MIENYRSGLLWNLFMSCPEVQAGLLKLGFSY"
     gene            complement(6092..7342)
                     /locus_tag="PKI35_06280"
     CDS             complement(6092..7342)
                     /locus_tag="PKI35_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNX44028.1"
                     /translation="MKKQILMFGALVLAIALLTGVNGCKKDEPTALTLSTLVAGSIDM
                     NGVTTPSNVPVSPSIVATFSTDVDPLTATSANIKLKRDYDAADIDLNISVNGKTITIE
                     PTEDLASGALHKLTFSASLKSKEGELLKTDLERTFTTAGFFAPTGMVAYWNFDTDAND
                     QVGSYNPSTTKDITYADGRKAEAGKAAQFNGTTSIIEIPNADPLITTHDFTISFWVKT
                     NSADITHGHFVMGLGAFYGLQFEINGSYKEAKFAIQYEEAEGKSASEDMWFPSNATDN
                     TNGGWQGWDYAKSLTDDEMIALLKDNWLHVVYTFNGEAKKGILYYNGEKMKSFDFNLW
                     PDGDAKKSVFGMKYAGVEPDVVNDLAFGFIQSRAGTMWDNETWGGYDFPDANHFKGML
                     DDIRIFHKTITPAEVLLIYNSEKP"
     gene            complement(7381..8868)
                     /locus_tag="PKI35_06285"
     CDS             complement(7381..8868)
                     /locus_tag="PKI35_06285"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX44029.1"
                     /translation="MKKTIYILAVASTLMMSLAFSGCEKFLEKDPQGSLTQTSFPTTP
                     DDALLAVNGVYATLRDIGYHSGVYPLCDIMSDDARKGSNPDDQASTIGPYDNFGFTTT
                     GSELAFWWATLYQGIKRANVVIEKVPAIVMDETLRNRYVAEAKFLRGLFYFDLVRAWG
                     GVPLITTLDPPMTLTRASKEAVYQQIETDLLDALKDLTEKTEIPALQQGRATKGAARA
                     LLAKVYLFEHDYVNAEKYAMEVINSGLYRLEPVFTDANGVNGEYGVESVFEVGSVGQE
                     SDQGCQYGNVQGVRGTPNRGWGFNRPTPDLRNSFEEGDPRLKGTIIDLGDIIDGDTIQ
                     GDGPTPDITLDEHGNVLETECYNRKVWTPGTNVPTQFGHNRRILRYADVLLMAAEALN
                     QNDKPDQALIYLNKVRERAREGNNAILPDITVTDKDALQEIILNERRHELALEGHRFW
                     DLVRTGKAAEVLGPLGFVAGKHELFPIPQTERDISQGAITQNPNW"
     gene            complement(8875..11895)
                     /locus_tag="PKI35_06290"
     CDS             complement(8875..11895)
                     /locus_tag="PKI35_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX44030.1"
                     /translation="MKQKIRFYNKMCLIAVLMLISAVSGAQVKTVTGTVTDNQTHETL
                     PGASIIIKGTTRGTTTDLDGKYSLEVRPGDITLVFSFIGYSNKEIPIGNQMIINTGLD
                     VEKTMLEEVVVIGYGTVRKSDLTGAVASVKAKELTRVTSLSPEQSLQGKVTGVQVTST
                     SGAPGEVPSVRIRGVGTFNNSAPIYVVDGVILDNISFLNSSDIASMEVLKDASATAIY
                     GSRGANGVILITTKSGSAGEEKTTFSYSGEMSMQTLAKKIDLLNGREFAIIANEIRPG
                     SYNNIDAVPNTDWQDLIFSTAPIQSHQFSASGGTKKMTYYIGIGYFNQQGIIDKSGYE
                     RLTLKLNNTYSLTDHIKFGNNITLAPYKQQNAPGVVFQAYRAWPALLPYRADGDFAGV
                     PGVGNPLADIEYTNNFNKGVRAVGNLFTEVTFLNNFTAKTSLGIDAGYNKSESFTPAF
                     QVYYYDGTASMQNSLKSSLSKGSSDNITWLWENTLNYNQSVNKHTISAVAGYTMQNSA
                     SEYLNVSGQNIIRDNSDFWYLTYSNITGTDVKASLENGVDVNLYYSMISYLARANYTY
                     DNKYIFTATFRRDGSSKFSKENRYSNFPSFALGWNISEEPFLSDVKQITKLKLRASWG
                     KIGNEKIVYSDRFSLTQPNLWAIFGSGDHPYTAISYAKSGNPDLRWETTTQTDIGLEV
                     GFLNSRLTGEFDYYHRVTDDILVELSTPGFMGNGQGQKIRYNAGKVLNSGFEANLNWR
                     DKTGDFTYSVGLLATTVHNEVLSIGGSSGIDSLLYGGDVYGYVTQSRKGLPIGAFWGY
                     KIDGVFQDQADLDAYPHAIDVQPGDLRRVDVNKDGKIDGSDRTYIGSPVPKFIFGLNG
                     EVEYKNFDVSISLQGQTGNKIFNAKEVIRPDQYNYEQHVMDRWTGPGTSNSEPRPTSG
                     GYNYLPSDFYIQDGSFARIRSVILGYTFPGELSKKIRVQQCRVYLKGNNVYTLTKFTG
                     YTPEIGSSDVLSNGIDTGIYPITAIYSVGLNITF"
     gene            complement(12353..12847)
                     /locus_tag="PKI35_06295"
     CDS             complement(12353..12847)
                     /locus_tag="PKI35_06295"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084140945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNX44031.1"
                     /translation="MELYKTHYSSPIGWIEISGSRQGIQSVIFLDREPEIQAEIPPCL
                     SECVRQLDEYFREKRKSFGLLLHPAGTEFQLRVWNQLQAIPYGETVSYLEIAGKTGNK
                     NSSRAVGHANGQNPINIIIPCHRVIGANGKLTGYGGGLWRKEWLLKFEKANTLPGLFK
                     NRFE"
     gene            complement(12891..14054)
                     /locus_tag="PKI35_06300"
     CDS             complement(12891..14054)
                     /locus_tag="PKI35_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNX44032.1"
                     /translation="MRNISFFVFFGIVILVYFLINYYIYIRGWQALPVNSGIRSWYRW
                     IFIFLASSYLIARILERIWLSHVSDVFTWTGSFWLGIMFYLLLLVAFIDLIRLLHHFA
                     PVYPFFLPSGPARLKLTVFVITLSTVIITAVIGYINAISPVIKTIELTIPKTAGHRST
                     LTIAMASDIHMGTLVGPRRTKKMAAMINRQKPDLVVFAGDLVDEELAPVLRFDLGREL
                     NKISAPLGVYAVTGNHEYIGGAGKAKKYLKDHGIIMLEDTSLLIDNSFYLAGREDRMK
                     NRIAGQPRKSLGDLLCQTDMTKPVIMLDHQPFDLDSVAAAGVDLQLSGHTHHGQLWPL
                     NYITRALYEVSMGYKKNGKTQFYVSPGYGGWGPPMRTNSRPEIVLLRISFKTP"
     gene            14337..15548
                     /locus_tag="PKI35_06305"
     CDS             14337..15548
                     /locus_tag="PKI35_06305"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HNX44033.1"
                     /translation="MTHSISMKATLKTAFFALALIIFTFNANAQAVLYQQTFEDSVHS
                     FQSFVLCEADHGIPADTSLSALADSAWVIRYIDTLNTYAALATSNYNPAAAANDWFIT
                     PAIPLGKASKLTFKAAASMAGPADSYSVYISTSQQDVNSCLLNPVLATYDGEPENEWT
                     EHTLDLAGAGYASQQVYLGFRLHTQTGGNNLMIDDITVTDDSVTNLVALTFNVNMSVW
                     IRDGKFKPKNDSIDIVGNFNNWTEGQYMLIDSIPATDSIYTITIPGFTVGQHLEFKFR
                     IDCSWADTLVEFPYGYPNRTWDIEPGKYTYSCYYNDEGTPHGLKENQPVMAEVKVFPN
                     PFSNEIRIINPEQVNRVVLTGSAGQRIAEWKCANGQDLDLNLGNLPGGIYMLLFYDQK
                     GYLGCRKISKF"
     gene            complement(15719..16174)
                     /locus_tag="PKI35_06310"
     CDS             complement(15719..16174)
                     /locus_tag="PKI35_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX44034.1"
                     /translation="MNNLKIRKAMPHHFEVIALFQVEMALETEGLVLDPQIVENGVKA
                     ILADSSKGCYYIAEIDGKITASLLITYEWSDWRNATVYWLQSVYVVKEFRQQGIFKAL
                     YGHIRNQVNEDPSVAGIRLYADKSNFSARKVYEKMGLSDEHYTTYEWMK"
     gene            16297..18198
                     /locus_tag="PKI35_06315"
     CDS             16297..18198
                     /locus_tag="PKI35_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNX44035.1"
                     /translation="MSDQIKHECGIALIRLLKPLEYYSARYGTTLYGLNKLHLLMEKQ
                     HNRGQDGAGVAGVKFDMKPGMRYINRIRSNAATPIEDIFRQIYGPIQEAARQNPERLH
                     DAQWLKENLEFTGELFLGHLRYGTFGKNNIENLHPVMRSNNWMTRNLVLAGNFNMTNV
                     DELFNRLVEFGQYPVETSDTITILEKIGHFLDEANEEIYYSLKEKGYSKKESTRLIGE
                     KLDLQNILSKSSTYWDGGYTIAGMIGHGDAFVIRDPAGIRPAFWYRDDEVVVVASERP
                     VIQTVFNVPASSVQEVNPGYALIIRKSGDVSEVMCKRPAEKKSCSFERIYFSRGTDVD
                     IYQERKKLGKLLVSDILKAIDYDIPNTVFSYIPNTAASAFYGLVEELDRYCDSVKKDK
                     IMLLGSGISREQLDEIFRLQVRIEKVAVKDIKMRTFITQDSKRDDLVPHVYDITYGTV
                     KPGTDNLVVIDDSVVRGTTLKRSIIRMLDRLQPRKIVIASSAPQIRYPDCYGIDMAKI
                     GDFIAFNAAIELLKETGQENILNEVYRKCKEQEELPKERIENFVKEIYAPFSDSQISA
                     KISALVTPQTCKASVEVVFQSIANLHLAIPGHAGDWYFTGDYPTPGGNKVVNQSFINY
                     MEGKNVRAY"
     gene            complement(18264..18656)
                     /locus_tag="PKI35_06320"
     CDS             complement(18264..18656)
                     /locus_tag="PKI35_06320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNX44036.1"
                     /translation="MASIFSRIASGEIPSHKVAENDRFFAFLDINPVAKGHVLVVPKK
                     EIDYIFDMETSEYAAFWEFAMEVARALKKAIPCIKVGIAVVGLEVPHAHIHLVPMNTM
                     NDLNFSKPKLQPSSEELASTAALIAKEM"
     gene            complement(18695..19171)
                     /gene="greA"
                     /locus_tag="PKI35_06325"
     CDS             complement(18695..19171)
                     /gene="greA"
                     /locus_tag="PKI35_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNX44037.1"
                     /translation="MAQTTYYTKEGLEKLKAELEHLTTVERPSISQQIAEARDKGDLS
                     ENAEYDAAKNAQGMLELKISKLQDLIRNARLIDESKLDNSKVLILSSVKIRNMKTNAV
                     MSYTLVPENEADLKAGRISITSPIAKGLLGKSKGDIVDILVPAGKISVEIVEISQK"
     gene            complement(19277..20332)
                     /locus_tag="PKI35_06330"
     CDS             complement(19277..20332)
                     /locus_tag="PKI35_06330"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNX44038.1"
                     /translation="MKNIYKAIIAFLLIGAVTLPVTLLAGNKDRAGQSGASELLINPW
                     ARSSGWGGVNVANAYGLESMYNNIAGLAFTKGTELIFSNTQYLKGSEITINNFGLSQK
                     LGESGVLGVAIMAMKFGDLPITTTSLPEGGIGNFSPSYMNINLSYAKAFSNSIYGGFN
                     LKIISESISDASAQGIALDAGIQYVTGAKENVHFGISLKNIGPTMRFKGDGLSIRSFL
                     PDNDNKFTLEQRSADFELPSQLNIGAAYDFHFSDMHRLTVAGTFTSNSFFKDQYTVGL
                     EYALMSYLQLRGAYTYEDGITSDEERTTVYTGPSAGLTVQIPLNKQKGSAFAVDYSYR
                     ATNPFEGTHSIAARLNF"
     gene            complement(20380..24549)
                     /locus_tag="PKI35_06335"
     CDS             complement(20380..24549)
                     /locus_tag="PKI35_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44039.1"
                     /translation="MKSLNRILPLILFFSLLVTGTFAERYHAEKSRSAKSSIKSTAAG
                     CLPGSNFKWLEINNVRTRIHSSGDMWWDFETAQYEIPKGSKKMSMFSGSLWLGGLDVN
                     NQLKLAALRYRQVGNDYWPGPLTVDGTASIDEVTCAKYDKLFEMSRADVDEFLAYWDD
                     PSSYPGYQIPASIKNWPAHGEKGQAEFLAPFYDRDGDLRYEPENDGDYPYYDVSNELC
                     HTKIPTAEGNGILVDQVIKGDYTLWWVFNDKGNIHTETEGEPIGVEIRGQAFGFSTND
                     EVNNMTFYSYEIINRSTYRLKETYFCQWVDTDLGFAGDDYVGCDVKRGLGYCYNGKPV
                     DGNGQAWAYGAQPPAIGVDYFQGPYMDPDGIDNPKFNPKTGENCDVSINGVNFANGIV
                     DDERFGMRRFVYHNNSGVPDYMTDPDVAIEYYNLMKGIWKDETHMIWGGNAHAQGGGV
                     GPDCDFMFPGNTDPCYWGTGGLPPNGMPYWTEETAGNDPNDRRFMQSAGPFTLEPGAV
                     NYITVGIPWARAASGGPFASVKLLQQTDDKCQILFDNCFKVISGPNAPDLTIRELDRK
                     LIIYLSNRKTNDAGNNYLEKYQEYDPRIVSPDTLGHANRWDSTYNFEGYQVFQLKNAT
                     ITSADIHDPAKARLVFQCDVKNDISRLINYNYDATLNGNIPVEEVNGANTGITHSFVA
                     TEDAFTGEKLVNHQQYYYLAIAYAYNSYQSYDPNSADLLTGQKLPYLPGRKNIKTYTG
                     IPHATVGMVEAKADYGQGVTITRIAGQGNGGNILDISKESVDEIMSKSPVDENNQLGS
                     DTYPIAYNLKYEKGKGPLDIRVIDPLNVKNAEYTVKFDPMFYSTVKVGEMDTLMMDGR
                     WTLTDNATGRVYKSDSLISYKNEQIFLDLGLSLTVSQIFDPGAFRLVKSYDTDGTIKY
                     TWGPAAENNGYLSAEIVYADSSNRWLSGVADVDGSPIYNWIRSGNVFDKDNKVNNDWD
                     MKGVDANNKAYDPNQVYENLIGGTWSPYNLSAGHDQMQSNLGPAYGAISYIGKRYSNM
                     YDLASVDIVLTSDKSKWTRSPILEMCPDQSLSENWAKQFELRKHRSVNQDGDTAVVSS
                     DPAKNSDYISAYGMGWFPGYAINLETGERLNIVFGEASNMVNDNGNDMLFNPSSREMD
                     AEGNPVMGGKHYIYIMSHTQDQFHQGLDSHYDNPAYDAGKNFVTQFNLTGTLAARYKV
                     IQMSNAMWVSMPLAVPSTSWLGCDATVKLRVGKPYRPNFSFTQTGDLAQAHNDNNFFP
                     MYTFTTEGVSTVTDNTKKAESDLDKINVVPNPYYAYSPYEADQLKNMVKIVNLPEHAV
                     VTIYTTSGTLIRKFPAKEASTTYIDWDLKNAAGIPISGGLYLIHVKVPGVGEKVVKWF
                     GVLRPVDLNAF"
     gene            complement(24602..28270)
                     /locus_tag="PKI35_06340"
     CDS             complement(24602..28270)
                     /locus_tag="PKI35_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX44040.1"
                     /translation="MVRKLLTVFVLLLSANIAVFAQSGTLKGKILDKTTKEPVPFANI
                     IAEANGTQAGGSTSDFDGNYTIKPLSPGKYNIKATYVGYKPVLIQGVLIKADQITPFN
                     IEMESTAIEITTYEVVDYKIPLIDKDKTTVGGTVTAEEISKMPSREVGSIVATVGGVF
                     SADGEVGSMRGQRTDGNVTYIDGIKVRGLSTLPQSAIEQVSVVLGGIPAQYGDATGGI
                     INVTTKGPSRTFGGGIELETSEFLDPYGYNRGGVNLTGPLFWNKDKTSSVVGYFLAAD
                     FEYKKDQDPLAIGNYKIKDDVLSYLEKNPLRPSGTGFGTYENAEFIRRSDLEHIKTNL
                     NNDGYSINTSGKFDIKTGPNINLTLGGSYNANSGHNFNWVQSLANYNRYSYTDQTTWR
                     LFGRFTQRFPSDKESRSPIKNVYYTIQGDFTRDHTKTYDPDFKDNLFAYGYVGKFTTY
                     KTRSFELDTTTAGFNGYVMNAYNDTRVNFDRAEYNPVLANYTSYYYSLYNTSAGHYDK
                     LDNIYSGGGMLNGDSPSDIYGMYASPGVLQSGYAVADVDQVTLNFSSSADVGNHEIKL
                     GFVFEQSKSSSYSYAPINFWTLMYQLANSHITQLDMSDPTTVYHEGGFDTTYYHRKYN
                     ASSQRAFDRNLRRLMGLAVDGTDWIDINSYDIDKQTINYYDADNKMHTMTLNSGLNVN
                     LFSADELLNSGNQVAAARGYDYYGNKLTSKPTIDDFYNATDADGNYTRLIAPFEPIYM
                     AGYIQDKFSFEDLVFNIGLRVDRYDANQSVLKDPYLLYPAKTVGEVTEFGAHPSGMGS
                     DYVVYVDNVTSPTRITGYRDGMNWYDAQGNVVTDPSLDISNGGLVSGGSISPYLTDPD
                     DKSITGKSFTDYEPQITVMPRISFSFPISDEALFYAHYDVLTQRPKSNSTFNPISYLF
                     WETENAPTFSNPNLKPERTVDYELGFQQKLNNTSSLNLSAFYREMRDQIQIYRYTGAY
                     PKTYYSYNNIDFGTVKGLTVTYDLRRTGNTRVRASYTLQFANGTGSNQETSKGLIQSG
                     QPNLRTLIPLNFDRRHAVNLNIDYRYGEGKEYNGPVIKGKKILENAGFNITVGGGSGT
                     PYTRSSKISFLGNQSIISGSINGSRLPWSFKIDGRVDKDFHIGTRKDAKGNVTDLYLN
                     VYLQVLNILNSENIMGVWAATGNPDDDGYLAADEYQAQIYSQLNPESYIDLYRLRANS
                     PYNYSSPRMIRLGLSFNF"
     gene            complement(28526..28825)
                     /locus_tag="PKI35_06345"
     CDS             complement(28526..28825)
                     /locus_tag="PKI35_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44041.1"
                     /translation="MPVFRSNKRWPKWLLTAFIVSFALFNTSCSLLRPGTREKVERKQ
                     AKEAQKAQTEYEKALDMHLKNQSKEARKMMKKTSKHAKKFNRYMKKPYNKGPKCS"
     gene            28908..29327
                     /locus_tag="PKI35_06350"
     CDS             28908..29327
                     /locus_tag="PKI35_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44042.1"
                     /translation="MRITIIYSAIALLLAILPFSCTKEGTSKIPYVYVDYYLYPNTLD
                     YIPQGGTIIYDGGYRGIIIYRASESEFMVYERCCPYDPENANAYVEPEPGGLTVIDSV
                     CMSGYILTDGSPYKGPSNVALRQYHYSYNGEVLHIYN"
BASE COUNT         7500 a         6814 c         7263 g         8341 t
ORIGIN      
        1 cattcggcaa atatacaaca cgaaataata aaattcggtt ataaccgaaa attattataa
       61 taaaactcgg ttataaccga aaaaaaccgg cttcatcagc cgacgctgtc tacttttcag
      121 ccgcatcccg gcagtaatac ttcctttttc tcagagcagc gtaaacgtct ttttcagttc
      181 agtggctgaa ctgccgccga taaagacttc gaagtctccg ggttcccaat tgtatttcag
      241 gtcataatta tagaatttca ggtcttcggg agaaagggta aaagttatgg tttgggtctc
      301 gccggccttc agaaatacct tccggaatcc tttcagctcc ttgaccggac gggtcacact
      361 gcccgccata tcgcggatat agagctggac aacctcggcg ccgtcgtatt taccgtcatt
      421 ggtaagatct atgcttgccg tcagcgtttg attccctttt aactgcgtgc tgctgagttt
      481 caggtcaccg taagtaaaat gggtatagct gagcccgtag ccgaaaggat aaagcgggtc
      541 gttggataca tccaggtagt tggaacggaa tttccggaac cattgtcctt cgggcagcgg
      601 cctgccggtg tttttatggt tgtaataaat ggggatctgg cctgcattct gcgggaacgt
      661 aaccggcagt tttcctgacg gattgacttt tcccagcagg acatcggcaa tggcattccc
      721 ggcttcggtt ccgccaaacc agacattcag aatcgccgga atgttttcat tttcccaggg
      781 aatcgccaga ggcctgccgg tgaacagcac cagtacaacc ggttttccgg tggacagtaa
      841 ggccttcaga aggtctttct ggttttcggg aagcgagata tcgctcctgc tcgaactttc
      901 acctgccatc tcggcagctt caccaagggc ggcaaccaca acatccgatg cagcagcaat
      961 cttaacagct tcatcgatca tttcctgcgg cggacgattg tcgaatcggg ttggcttccc
     1021 gaagacgctg atgcgggcat cgagctgagg gtcgttcacg atattggttc cccttgcgat
     1081 caggatttca gcctggtcgc cgacggcatt cttcagccct tcgaggagcg aaaccgattg
     1141 ggaatagtcg gaggctacgc tccaggtgcc ggtcatgttt tccctgttgt cggccaacgg
     1201 gccgatgagg gctatcttcc cttttgcctg aagcgggagg acctgatttt catttttcag
     1261 caggacgaag gtccgggccg cagtttcgcg ggcgatcttt ctgtgttccg gggtgaaaat
     1321 ttcggttttg gcgcggttat catcgcagta cctgtaagga tcgtcgaaca ggccaagttt
     1381 gtatttggct tcgaggaccc tgcggcatgc ctggtcgatt tcggtctggg ttacctttcc
     1441 ttcctgtaac gatttaccga gcgttgtcag gagaccttca cccaccatgt ccatatcgac
     1501 gcctgcatgc agggccaatg ctgaaacggt ttgcagatcc cccatcccgt gatcgatcat
     1561 ttcgttaata gccgtgtagt cggtaacaac aaaaccatta aaaccccact gatggcgcag
     1621 caggtcagta aggagccatc tgttggcggt agccgggatg ccgtctattt cgttgaacga
     1681 cgtcattacg cttccggcgc cggcatctac ggcggctttg tacggcggga gatactcgtt
     1741 atacatgcgt atacggctca tgtcggtggt gttataatcg cgtcctgctt ccgacgctcc
     1801 atacaatgca aaatgtttta cacaggacat gatggtattg tttttacgca ggtcgtcacc
     1861 ctggtaacct ttgacaaagg ctttagccac ctgcgatcca agatagggat cttcgccggc
     1921 gccctcggca attcgtcccc agcgcgggtc acggcagata tccgccatgg gtgaaaatgt
     1981 ccagcagata ccatcggcgc tggcttcaat agctgctatc cgggcgcttt gttcgatcag
     2041 cggcaaatcc caacttgcgg ccagtcccag gggaataggg aaggttgttt gatatccgtg
     2101 gataacgtcc atgccaaaga ttaagggaat tttaagacga ctttggtcaa tggccagttt
     2161 ttgcatttca cggacttttt caacggattt gatattgaaa agtccgccta cttttccctc
     2221 tttaattttt ttgcctatat cggagctgcc catctcgccg gtaacaaact gaccagcatc
     2281 gggaaggttt aactgcccca gtttttcttc cagggtcatt ttcttcatca gggcgtcgat
     2341 aaataccttt attttctgat cagcagcctg gggtttggtt tgggccgaaa ccgaaattcc
     2401 ggaaagcaaa agagccccca acaacagagc ctttattcgt ttcataatcg tacgattttg
     2461 ttaaagctgt ttttatgatt cttaagtgga tctatatcag attcatattt ttcagaaagc
     2521 tgcagataca ggctttaaac cggagccggg cttttcagga attgtgagac tacccattaa
     2581 taccggtatg cccattaatc cagcctgcaa ctctttaaat ttagtcagta atttatttac
     2641 ggagccacgg ggattcgaat cccagtttct ttaatccagc ctgaatttcg gggcattgca
     2701 tgaaaagttt ccacaacaat tccgaacggt aattctctat cattacgaca atcggcccct
     2761 catcaatagc aagataacgg gccggatacc agttgttttg ttcgctgaaa gcgtcgtaga
     2821 atccatattt tccccatatt ttatctccca gatcgaaata aaaatgtttc agcgcctgca
     2881 tggaatattc aggggtatat gggaacgaag ataaggcagc cgtcggagaa attaccccca
     2941 gatcggtgtt tttgcccggt gcgtgggcgc tatagccaac ggtggaataa ctggccgtga
     3001 gtccccagca attttcgcca tagcctttaa aatggtttgg attttcgatg caccattgcc
     3061 tgttgatgag agtatggttt acattgttgg accagtagtc ggcatattca tctttcagtc
     3121 cgcggggatc cagccccagg aaggagtatt gcgaccagaa aagcgggccg ccgtattctt
     3181 cagcgccatt atgccgcaag gtgagttcat aaccatattt accgcttcgt gttttgattt
     3241 ttccgtccct ggcccagcct tttttatagg cgccggccgt aaccggatag gtgggagacg
     3301 aagccgccat tacgtaaaga atcaggcact cattatatcc ttcaaccgga aaattcatag
     3361 cccaaccata ggtaggggac caatgccagt aaagcacatc tttccccccg ttggtgaacc
     3421 agttccattc gacgcctttc cataatttgt cgatgtcgga cgacagtttt cgttcatcag
     3481 catttccgtt tttaaagtat tccctcacgc atagcatacc ctgcatgagg taggcggttt
     3541 ccacgaggtc agccccgtta tccttctgtc cgaaaggttt aacttttccc gtttcaccgt
     3601 acatccagtg aggccaggcg ccgtgaaagc ggtcggcttt cgaaagccag tcaacgctct
     3661 tgcggagctg tttataccct tcttccctgg taataaagcc ccgttcgatg ccaaccagaa
     3721 tagccatcac gccaaaaccg gaacctccgg aggtaatgac cgactggtcg tgttccgggt
     3781 atatcccgtc ggtatgaatt ctttcacagg ccagtcctga aaccggttca gctccttcgc
     3841 ggaaatactg gaaagtcctg tattcaacca gcgtcagcag cgaatcatcc gaaatttcgg
     3901 gccgggttgt tacgtttcct gatacagccg ttttcttctg atgaaaagaa caggacatca
     3961 ggataatggc agccagcagt gctgtggtat gcatggcgat agatttcatg atctcgagat
     4021 tgttgccggt taagaaatga tggacaatgg tatcagtagc tgaatcccag tttcagaagg
     4081 cccgcctgga cttcggggca gctcatgaag aggttccaga gtaatcctga acggtaattc
     4141 tctatcatac atatttccgg tccctggtcg atagcgatgt aggaaccggc ccaccagttg
     4201 ctgtggacgt cgaaggcatc ccgaaagccg tactcacccc acagccggtc gccaagaata
     4261 taatagaaga accggattgc gttcatggat tgctccggtg aataaggcaa tgatgaaacg
     4321 gcggcggtag gggcaataac tccgcggtcg ttggcaggtt cgcttacgcc atagcctgaa
     4381 ggaatatcgc tggcggtaag cccccagcag taagcgttgt aattcggata atttttcgga
     4441 ttatcggtgc aataggccca gttgataagg gaatgattca cattctggat ccagtagccg
     4501 gcataggcat ccgataaatt cctcgggtca aggcccagga aggaataatg ggcaaagaac
     4561 aatggcccgc cgtaatcgta tccaaccggg aggacataac cataaaacgt tttcccgttc
     4621 ttaattccgc cgttcagggc aaaaccctgg tgataggcgg cagccgggat ggtatggaca
     4681 ggcgatgaag cggcaaggat gtaagtaatc agggtttcgt tgtaaccatg aatcgggaaa
     4741 ttcatgtccc agccgtaatt gggcgaccag tgccagtaca atacgttttg tcctccccgg
     4801 gtataccagt cccattcgat gccgttatag agcgttgtga tccggttgat aaggctgttc
     4861 tcgtcagcat cggcaggatt caggtattgg cgcatggtaa ggaggccctg tgccatgaag
     4921 gctgtttcta ccaggtcgcc gccatcgtcc ttggtgccga aagggatcac tgccccggtg
     4981 ttcccgttgc tccagtgcgg ccaggcgccg tgaaaacggt cagaggtttc gaggtaactg
     5041 atgatttttc caagtcttgt tgtcccttcc gaacggctga taaaaccacg ttccataccc
     5101 acaatcagag ccatgatgcc gaaaccggaa ccgccggttg tgacaatatc gcctgaaccg
     5161 gaacgttcgc gtgccatgcc gcaggcagga tgggcataat cataaaagta cctgaaagtt
     5221 tgctgctgga ttgccgtcag cagttcctcg tcggtgacca gggggaattt gagggttgaa
     5281 tcaattccgg tatagaatgc gttgctgaaa ccgctgaacg ataatccgtt ttttgatttt
     5341 aacgcactgg atatgttgaa atagtaacgc caaagatatt ttaatgccga attattggtt
     5401 atggtgactt ttgtatcatt atccgataaa gaacaggaaa tacccggatt ttcggctaac
     5461 gacatatagg atttgtaaga agccgggtcg agcgcgtgtg aaaaggaaac ttcgatatta
     5521 acagacttat aatcaatgtt ccgtaatgaa acatgggaag aaaaatcctg accgtttaag
     5581 gttattgatc gtaggataag ctgtccggct accgttataa aagtgaattc catggtctgg
     5641 aatgtttcgc ctgccgctcc ttttaacgaa cccgaaatgc ggatggtata atatttccgg
     5701 ctttctaaaa gttcgacggg tttcaatgtt attattttac catcggctga aaatccggaa
     5761 gtagttgata ccgtactgtt atcttttttt aataggatat tttcctttgc tgaaacggtg
     5821 tcgagggcac tgccgaactc cagcgtaatc ctgctgtcgg caggcacatt ttctgtggta
     5881 tcctgaagtt taaaaacaac atctccggcc tttatttgta tcagttgcag cgttccttcc
     5941 ggaacctgtt cttttttgca ggcgctttcc agcagcacac ctgccgcaat gattactaaa
     6001 aatgccggaa tgactttcat actcgtctga ttaagataga gagggttggg aattaaccca
     6061 accctcagaa tcaaagcaat tcaggttaaa attaaggctt ttcggaattg tagatcaaca
     6121 ggacttctgc cggagtgatg gtcttgtgga agatacggat atcatcgagc attcctttaa
     6181 agtggtttgc atcgggaaag tcgtatccgc cccaggtttc attatcccac attgtaccgg
     6241 cccttgactg aataaatccg aatgccagat cattgacaac atcgggctca acaccggcat
     6301 atttcatacc aaaaacggat ttctttgcat cgccgtcggg ccacaggttg aaatcgaagc
     6361 tcttcatctt ttcgccgttg taatacagga tgcctttttt agcttcgccg ttgaaggtat
     6421 acacaacatg gagccagttg tctttcagca gggctatcat ttcgtcatcc gtcagcgatt
     6481 tggcataatc ccatccctgc catccgccgt tcgtattatc cgtggcattg ctggggaacc
     6541 acatatcctc ggaggcgctt tttccttctg cttcttcata ctgaatggca aattttgctt
     6601 ctttatacga accgttaatt tcaaactgaa gaccgtagaa tgcgcccaag cccattacaa
     6661 aatgcccgtg ggtgatatct gccgaattgg tctttaccca gaatgagata gtgaaatcgt
     6721 gcgtagtgat taaaggatca gcattgggga tttcaataat acttgtggtc ccgttaaact
     6781 gagctgcttt accggcttcg gctttgcggc catcggcata ggtaatgtct ttggtagttg
     6841 atggattata ggagccgacc tggtcattgg catcagtgtc aaaattccag taggcaacca
     6901 tacctgtcgg ggcgaagaat ccggctgtgg taaaggtcct ttcgagatcc gtcttcagca
     6961 attccccttc ttttgatttc agggaagcgg agaaggtaag tttatgaagc gcgccactgg
     7021 cgaggtcttc ggttggttcg atggtgatcg ttttcccgtt gacgctgatg ttcagatcaa
     7081 tatctgctgc atcgtaatcg cgtttcagtt tgatattggc gcttgtagct gtcagcgggt
     7141 caacatcggt ggaaaaagtg gcaacgatgg aaggactgac aggaacattg gaaggcgtcg
     7201 tcactccgtt catgtcgatg gagccggcaa ccagggtaga gagtgtaagg gctgttggtt
     7261 cgtctttttt acagccgttt acgcctgtca ggagcgcgat ggctaacacc agcgctccaa
     7321 acatgaggat ttgttttttc ataagtaatt gtgatttaag gtttaacatt atattgatga
     7381 ttaccaattt ggattctgag ttatggctcc ctgggaaata tctctttccg tttgagggat
     7441 tggaaacaat tcatgtttgc cggcaacaaa ccccagcggg cctaacactt cggctgcctt
     7501 tccggtgcgg acaaggtccc agaaccgatg cccttcaagc gccagttcat gccggcgttc
     7561 gttaaggatg atctcctgta aggcgtcttt atcggtcaca gttatatcgg ggagtatggc
     7621 attgttaccc tcgcgggcgc gttctctcac cttgttgaga tatatcaggg cctggtcggg
     7681 tttgtcgttt tggttgagcg cttcggcagc cattagcagt acatcggcat accgcagaat
     7741 acgacggtta tgcccgaatt gtgtagggac attggtaccg ggagtccaga ctttcctgtt
     7801 atagcattcg gtctcaagta cgttgccatg ttcatccagg gtaatatcgg gcgttggccc
     7861 gtcgccctgg attgtatcgc cgtcgatgat gtcgcccaga tcaatgatgg tgcctttcag
     7921 acggggatct ccttcttcga aagaatttcg caggtcggga gtaggccggt tgaagcccca
     7981 accccggttc ggtgttcctc ttacgccctg aacgttacca tattggcatc cctgatcact
     8041 ttcctggccg accgatccaa cctcgaagac cgattcgacg ccatattctc cgtttacccc
     8101 attggcatcc gtaaatacgg gttcgagcct gtataaaccg gaattaatca cttccatcgc
     8161 gtatttttca gcatttacat aatcatgctc aaacaggtaa actttagcca gcagggcgcg
     8221 ggcagctcct ttggtagcac gcccttgttg taatgcagga atttctgttt tttcggtgag
     8281 atctttcagg gcgtcaagca ggtcggtttc aatttgctga taaacagctt ctttcgaagc
     8341 ccgtgtaagc gtcatcgggg gatcaagggt tgtgatcaac gggacgcctc cccaggcgcg
     8401 gacaaggtcg aagtagaaca acccgcgcag aaattttgct tctgcaacat aacggtttcg
     8461 taaagtctcg tccatcacga tggcaggtac tttttcaatg accacattgg cacgtttgat
     8521 gccctggtac aaggttgccc accagaaggc cagttcgctt cctgtagtcg taaacccgaa
     8581 gttatcgtaa ggtccgatgg ttgatgcctg gtcatcggga ttgcttccct tgcgggcatc
     8641 gtccgacatg atatcgcaca gggggtatac gccggaatga tatccgatat cgcgcaaggt
     8701 tgcataaacc ccgttcacgg ccagtaatgc atcatcggga gtggtcggga atgaagtctg
     8761 ggtaagggaa ccctgcgggt ctttctccag aaatttttca catcctgaaa aagccagcga
     8821 catcatcaat gtcgatgcta cggcaagtat atagattgtc tttttcatgg cctgttaaaa
     8881 tgttatgttc aaaccaacgg aataaatggc ggtaatgggg taaatgccgg tgtcgatacc
     8941 attggacagc acatcgctgc tgccgatctc cggagtatat cccgtgaact ttgtcagggt
     9001 ataaacattg tttcctttca gatacacgcg gcattgctgt acgcggattt ttttactgag
     9061 ttctccgggg aaggtataac ccaggatgac actgcgtatt ctggcgaagg atccatcctg
     9121 gatgtagaag tcggatggca ggtaattata gcctcctgag gtaggcctcg gttcgctatt
     9181 gctggtaccg ggacctgtcc accggtccat aacgtgttgt tcgtaattgt actgatcggg
     9241 ccggattact tccttggcat tgaatatttt gttgccggtc tggccctgga ggcttatgga
     9301 aacgtcaaag tttttgtact caacttctcc gtttaatcca aagataaact ttggaacggg
     9361 cgatccgata taggtacggt cgctaccgtc gattttaccg tccttattga catccacacg
     9421 gcgtaaatcg ccgggctgaa catcgatggc gtgcggatag gcgtccagat cggcctgatc
     9481 ctggaaaacg ccgtctatct tatatcccca gaaagctcct atcggcagcc ctttacggct
     9541 ctgggtcaca tagccgtaaa catctccgcc gtataacagt gaatcgattc cgctgcttcc
     9601 gccgatactc agtacttcgt tatggacggt tgtagcaagc agtcccacac tgtaggtaaa
     9661 atcccctgtt ttgtcgcgcc agttcaggtt ggcttcaaag ccgctattca gcactttgcc
     9721 ggcattgtat cgtatcttct ggccttgtcc gttgcccata aagccgggag tggacaattc
     9781 aaccaggata tcgtcggtaa cgcgatggta ataatcaaac tcaccagtca ggcggctgtt
     9841 cagaaatccg acttcaagcc cgatatccgt ctgggttgtc gtttcccacc ggaggtccgg
     9901 attcccgctt ttggcatacg aaatggctgt gtaaggatga tcgccgcttc cgaatattgc
     9961 ccaaaggttc ggctgagtaa gagaaaaccg gtcggaataa acgatcttct cattcccgat
    10021 tttaccccag cttgcacgca gttttagttt tgtgatttgc ttaacatcac tgagaaaagg
    10081 ctcttccgaa atattccatc ctaaagcaaa agaaggaaaa ttgctgtaac ggttctcttt
    10141 ggaaaacttc gatgatccgt cacgtcgaaa ggtagcggtg aaaatatact tgttatcgta
    10201 agtataattt gcccttgcca ggtatgatat catcgaatag taaagattga catcaactcc
    10261 gttctccagg ctggctttga catcggtgcc ggtaatgttg ctgtaggtga ggtaccagaa
    10321 atcggaattg tcgcggatga tattctggcc tgaaacgtta agatactctg aagctgaatt
    10381 ttgcatggta taaccggcca cggcgctgat ggtatgcttg ttgaccgact ggttataatt
    10441 cagcgtgttt tcccataacc aggttatatt atcggatgat cccttggaga ggctgctttt
    10501 taaactattt tgcatggaag cagttccgtc ataataataa acctggaagg ccggagtgaa
    10561 actttccgat ttgttgtaac cggcatcaat gccaaggctc gtcttggccg tgaagttatt
    10621 caggaaggtg acttcggtaa acagattgcc cacggcacgt acacccttgt taaaattgtt
    10681 tgtgtattca atatcggcaa gcgggttacc cactcccgga acgccggcaa aatcaccatc
    10741 ggcacgatag ggcaggaggg caggccatgc cctgtaagcc tggaatacta ccccgggcgc
    10801 attttgctgt ttgtaaggtg caagagtaat attattgccg aatttgatat ggtcggtgag
    10861 gctgtatgtg ttattaagct ttaaggtaag cctttcataa cccgatttat cgataatgcc
    10921 ctgttggttg aaatacccga tcccgatgta atacgtcatt ttttttgtac cgccgctggc
    10981 cgaaaactgg tggctctgaa tgggcgccgt gctaaagata aggtcctgcc aatcggtatt
    11041 gggaacggcg tcaatattat tatagcttcc cgggcggatt tcattggcaa tgatggcaaa
    11101 ctcccttccg ttgaggaggt cgattttttt tgccagcgtt tgcatgctca tttctcccga
    11161 ataactgaat gttgttttct cttcaccggc cgaaccgctt tttgttgtta tcaggattac
    11221 gccgttggcg ccccttgagc catagatggc ggttgctgat gcatctttga gaacttccat
    11281 cgaagcaata tcggaagagt tcaggaatga tatgttgtcg agaatgaccc cgtcaacaac
    11341 atagatgggc gcagaattgt taaaagttcc cacgcccctg atcctgaccg agggcacttc
    11401 acctggcgct cctgatgtac ttgtaacctg cacgccggtt acttttccct gaaggctttg
    11461 ctccgggctt agcgaagtaa ccctggttag ttcctttgct ttaacggaag cgacagcgcc
    11521 ggtgaggtcg cttttcctga cagtcccgta accgataact accacttcct ccagcatggt
    11581 tttttcaaca tcgagcccgg tattgatgat catttggtta ccgatgggga tttccttgtt
    11641 gctgtatcct ataaaagaaa atacaagcgt aatatctccc ggccttactt caagggaata
    11701 ttttccgtcc agatcggttg ttgtgccccg tgtagtacct ttaataatga tagaagcgcc
    11761 gggcaacgtt tcgtgggtct gattgtcagt tactgttcct gtaacagttt tcacctgtgc
    11821 cccggaaacc gcgctaatca gcattaacac tgcaatcagg cacattttat tgtaaaagcg
    11881 tatcttttgt ttcatggctt aaacaacagg ttagttttgg ttaattaaca gttgagcata
    11941 tgggaattgc agcaattaat tacagtatac aggtaagttt ataactatac cttaaaaata
    12001 caacaaaatc aggcttaaaa atacggtaaa ttgagaattg ctttactata tccataattt
    12061 aaatattccc ggataagaat tttcatgctg aaataattga taatatcaga gtgaaaagat
    12121 gtaatgtaaa ttcagaacat ataaacaatt cattatgatt ctgatagcta tcctacgacc
    12181 ggaatcctgc ttctcaggaa tcaaaattca tcaaagtgaa aatatttatc cgggatccgg
    12241 cgcttttagc ccgatcgctg gtctttatag tttataacct gtaacctgaa tttcagcgac
    12301 tcaggcttct gaatccttat taccggacaa taacgaaaat cccggatgtt tttcattcga
    12361 agcggttttt aaaaagtccc ggaagggtat ttgctttttc aaatttgagc agccattcct
    12421 tacgccacaa accgcctccg tatcctgtga gtttaccgtt tgcccctata acccgatggc
    12481 agggtataat aatgttgatc gggttttgtc cgtttgcgtg cccgacagcc cttgacgaat
    12541 ttttattgcc ggttttcccg gcaatttcca gataggatac ggtttcacca tacgggatcg
    12601 cctgcaactg gttccatacc ctgagctgaa attctgttcc tgcggggtga agcagcaatc
    12661 caaaggactt tcttttctcc ctgaagtatt cgtccagttg ccgaacgcac tccgataaac
    12721 agggaggtat ttcagcctgt atttcaggtt cccgatcgag gaatattacc gattgaatgc
    12781 cttgccggct tccgctgatt tcaatccagc ctatcggaga ggaataatgt gttttgtata
    12841 gttccataca aaggtaacct gaagtattgt tatctgataa gtggcttgtt tcagggcgtt
    12901 ttgaaagaaa tcctgagcag gacaatttcc ggccggctgt tggtgcgcat gggcgggccc
    12961 cagcctccgt atccggggga aacatagaat tgagttttcc cgtttttttt atatcccatg
    13021 cttacctcgt aaagggcgcg ggtaatataa ttgaggggcc agagctgacc gtgatgggta
    13081 tggcccgaca attgaagatc aactcccgct gcagccaccg aatcaaggtc gaagggctgg
    13141 tggtcgagca taatgacagg tttcgtcata tccgtctggc aaagaagatc gccaagggat
    13201 ttcctgggtt ggcctgctat ccggtttttc atccggtcct cccttcctgc aaggtaaaaa
    13261 ctgttgtcga tgagcagtga ggtatcttca agcatgatga tgccatgatc tttcaggtat
    13321 ttttttgctt tgccggcgcc gccgatatat tcatgatttc cggtcacggc atacacaccg
    13381 agcggagcgg agattttgtt caattccctt cccaaatcaa aacgcaaaac cggcgccagt
    13441 tcttcatcca ccaggtcgcc ggcaaatact accaggtcgg gtttctgccg gttgatcatg
    13501 gctgccatct tcttggtccg gcgcggcccg acaagcgtcc ccatgtgaat atccgaggcc
    13561 atcgctatcg taagggtact cctgtggccg gccgttttag gaatcgtgag ttcaatggtt
    13621 ttaatcaccg gagatatggc attgatataa ccgataacgg ctgttatgat gacggttgaa
    13681 agggtaatta caaaaaccgt cagctttaat ctggccggtc cggaaggaag aaagaacgga
    13741 taaaccgggg caaaatggtg caaaaggcga ataaggtcaa taaaggctac cagcagtaaa
    13801 agataaaaca taatacccag ccagaatgat cctgtccagg tgaaaacgtc gcttacatgc
    13861 gaaagccaga tccgttcgag gatgcgggca attaagtaag aggaagccag aaaaatgaaa
    13921 atccatctgt accagcttct tattcctgaa tttacaggca aagcctgcca tcccctgata
    13981 tagatgtaat aattgatcag gaaatatacc aggatgacga taccgaagaa aacgaagaag
    14041 gatatattcc gcatcggtta acagcaggtt ttgggaaagt tgtatccggt ttttatccgt
    14101 cgaaccaaac caacgaatgt gtaaatttac ggtttaaaat ttcgaccaga ttcaggaatt
    14161 aaaaaagaaa tgataaggtc gttattaatc ggaaaaagat aattcaggtg cagaatttga
    14221 gataattatc ttctcctttc ggaaagaata gcaatacatg aagcaaaact gaacagaaga
    14281 cgggaccaaa aaggccgttt aaaaagtttc tgaaatcaat aaactgaatc aattccatga
    14341 cccactccat atccatgaaa gctaccttaa aaaccgcatt ctttgcttta gcgctgatta
    14401 ttttcacttt caatgccaat gcccaggctg ttttgtatca acagactttt gaagacagtg
    14461 tgcactcatt tcagtcattt gttctctgtg aggctgacca cggcattccg gccgacacaa
    14521 gcctgtcagc cctggccgac agcgcctggg taatccggta tattgatact ttaaatactt
    14581 atgctgcttt ggctacgtcg aattacaacc ctgctgcggc tgccaatgat tggttcatta
    14641 ctcccgccat accgcttggg aaggcttcaa aactaacctt taaagccgct gcatcaatgg
    14701 ccgggccggc cgatagttat tccgtttaca tctcaacttc acagcaggat gtgaacagtt
    14761 gccttcttaa ccctgtactg gcaacttatg atggagaacc tgaaaatgaa tggactgagc
    14821 atacccttga ccttgccggt gccggttatg cttcgcagca ggtatatctg ggcttcaggt
    14881 tacataccca aactggcgga aacaatttga tgatcgacga tataaccgtt acggatgatt
    14941 cggtaacaaa ccttgtggct ttgactttta atgtaaatat gtcggtttgg atcagggatg
    15001 gaaagtttaa accgaagaat gattcaatag atattgtcgg aaatttcaac aactggaccg
    15061 aaggtcaata tatgcttatt gattcaatac cggcaacgga ttcaatatac accattacca
    15121 ttccgggctt tacagttggg cagcacctgg aattcaaatt caggattgat tgcagttggg
    15181 ccgatacgct ggtggaattt ccctatggtt acccgaaccg gacctgggat attgaaccgg
    15241 ggaaatacac ttactcctgc tattacaacg atgaaggaac tccgcatgga ttaaaagaaa
    15301 atcaaccggt aatggctgaa gtaaaagtat ttccgaatcc tttcagtaac gaaatcagga
    15361 taataaatcc cgaacaggta aacagggttg tgctcaccgg gtcggcgggt cagagaattg
    15421 ctgaatggaa atgtgcgaat ggtcaggatc ttgatctgaa tttgggcaac cttccgggtg
    15481 gtatttacat gctgctgttt tacgatcaaa aagggtatct gggctgccgt aaaatatcaa
    15541 agttctgata gttgttacga taatacggga ttctgtctaa aaatccggaa gcctggaatt
    15601 tggaatccgg aaaccggagc cccggaaaag tgtagaaaat tctgtactga caggaaagag
    15661 gaatccggca acaggaaaca ctcttcatat tccctgtaat ttgtgtaatg aaacctgcct
    15721 acttcatcca ctcataagta gtgtagtgtt cgtcgctcaa ccccattttt tcataaactt
    15781 ttcgggcgct gaagtttgat ttgtcggcat acagacgaat tcctgcaacg gatggatctt
    15841 cattaacctg atttctgata tggccgtata aagccttgaa aattccctgt tgcctgaatt
    15901 ctttcacaac ataaaccgac tgaagccagt aaacggttgc atttcgccag tcgctccatt
    15961 cataggttat aagaagggaa gctgttattt ttccatcaat ttcggcaata taatagcatc
    16021 ctttggaaga atctgcaaga attgctttaa cgccgttttc tacaatttga ggatccagta
    16081 caagaccttc ggtttcaagg gccatttcaa cctggaacaa ggctattact tcaaagtgat
    16141 gcggcatggc ttttctgatc ttcaggttgt tcataagcat cgttattttt taccggaacg
    16201 aatgagtgcc gaaacgggca tagggaaatg cttatttttg cacttctttt tcgttcaatg
    16261 ctgccaaaag tagcaaaaac cttaaaccga gcgtcgatga gtgaccagat caagcacgaa
    16321 tgcggcattg ccctgataag gctgctgaag cccctggaat attattcagc aaggtatgga
    16381 acaaccttat atggtctgaa taagctgcac cttttaatgg aaaagcagca caaccgtgga
    16441 caggatggcg ccggtgttgc cggcgtcaaa ttcgatatga aaccgggaat gcgttacatt
    16501 aaccgcatca ggtctaatgc cgccacgccg attgaggata tattcaggca gatttatgga
    16561 cctattcagg aagctgccag gcaaaatcct gaaaggcttc acgacgccca atggctgaaa
    16621 gaaaacctgg agtttaccgg tgaactattt ctcggccatt tacgttacgg cacgttcggg
    16681 aagaataata tcgaaaacct tcatcctgtg atgcggtcaa acaactggat gacacgaaac
    16741 ctggtacttg ccggcaactt caatatgacc aatgtggatg agttgtttaa ccgccttgtt
    16801 gagtttggcc agtatcctgt tgaaacttcc gacacaatca ccattctgga gaaaattggc
    16861 cattttcttg atgaagccaa cgaggagatt tactacagct taaaagaaaa aggctattcc
    16921 aaaaaggaaa gtaccaggct gattggagaa aagctggact tgcagaatat cctttcaaaa
    16981 tcttccacct attgggatgg cggttacacc attgccggga tgattggaca tggggatgcc
    17041 tttgtgatcc gtgatcctgc aggcatccgc ccggctttct ggtaccggga tgatgaagtg
    17101 gtggtggtgg catccgagcg tcctgtaata caaactgttt tcaatgttcc ggcttcctcg
    17161 gttcaggagg taaatccggg ctatgccctt atcatacgca aaagcggaga tgtatccgag
    17221 gtaatgtgta aacgtccggc tgaaaagaaa tcctgttctt tcgaaaggat atactttagc
    17281 cggggcaccg atgttgatat ttatcaggag cgtaaaaagc tcgggaaact actggtttca
    17341 gatatcctaa aagccattga ttacgatatt ccgaacacgg ttttctctta tattccgaat
    17401 acggccgctt ctgctttcta tggacttgtg gaagaactgg accgctattg cgactcggtg
    17461 aaaaaggata aaataatgtt gctgggctcc ggtatttcac gggaacaact ggatgaaatt
    17521 ttcaggcttc aggtccggat tgaaaaagtt gcggttaaag atatcaagat gcggacattt
    17581 atcacgcagg attcaaaacg cgacgacctg gtaccgcatg tttatgatat aacttacgga
    17641 acagtaaagc ccggaactga taacctggtt gtaattgacg attctgtggt acggggaacg
    17701 acgcttaagc gaagcatcat caggatgctg gaccggcttc agccaaggaa aatcgtaatt
    17761 gcatcctcgg caccgcaaat tcgttatcct gactgttatg gcattgatat ggccaaaatc
    17821 ggggatttta ttgcttttaa tgcagccatt gaattactga aagaaactgg tcaggaaaat
    17881 attcttaatg aagtttacag gaaatgtaaa gaacaggaag agttgcccaa ggaacgaatt
    17941 gaaaattttg tcaaagaaat ctatgcgcca ttttctgaca gccagatttc agcaaagatt
    18001 tcagcccttg taacgcctca aacctgcaaa gcttcggttg aagttgtttt ccagtcgatc
    18061 gcaaaccttc atctggctat tcccgggcat gccggcgact ggtattttac cggcgattat
    18121 cctacgccgg gaggtaacaa agtggtaaat cagtcgttca tcaactatat ggaagggaaa
    18181 aatgtgaggg cttattaatc tgcctgtata aaacagaaaa ggcggccaga aatggccgcc
    18241 ttttctttaa gcctgtcagt gatttacatt tctttcgcga taagggccgc tgtagaagcc
    18301 agttcctcgg atgaaggctg aagtttgggt ttcgaaaagt tcagatcgtt catcgtgttc
    18361 atcggtacca ggtgtatgtg tgcatggggc acttcaagac caacaacggc aatcccgact
    18421 ttaatgcaag ggatcgcttt tttaagcgct ctggccacct ccatggcgaa ttcccagaaa
    18481 gcggcgtact ctgatgtttc catatcaaag atatagtcaa tctccttttt gggtactacc
    18541 aggacatgtc cttttgcaac gggattaatg tcgaggaaag caaagaaacg gtcattttcg
    18601 gctactttat gactcggtat ttcgccggac gctattcgtg aaaaaatgga agccatgatt
    18661 atttgtttta aaaggcaaag ttaacgtcag gaaattactt ttgggaaatt tcaacaattt
    18721 caaccgagat ttttccggca ggcaccaaaa tatcaactat atcgcctttg cttttgccaa
    18781 gcaatccttt ggcgatggga gaggtgatgg aaattctgcc cgctttcagg tcggcctcgt
    18841 tttcaggaac cagggtatag gacataaccg cattggtttt catgttcctg atcttaacgc
    18901 tactaagtat cagcactttc gaattatcga gtttcgattc gtcaataagg cgggcgttgc
    18961 ggatcagatc ctgaagtttg gaaatcttca gttcgagcat accctgggcg tttttggcgg
    19021 catcgtattc ggcgttttcc gaaagatcgc ctttatcgcg ggcttcggct atctgttgcg
    19081 atattgaagg ccgttcaacg gttgtcagat gttctaactc agctttaagt ttttcaagac
    19141 cttctttggt atagtaggta gtctgtgcca tgatgcaata atttcttttc gattaataag
    19201 tgaaaaagac ccttatagga aagggtcttt cattaaaggc aaaagtaagc aggaaaatta
    19261 ttcccggctt atgttgttaa aaattcaacc gggctgcgat gctgtgcgtt ccttcgaacg
    19321 gattggtagc cctgtatgag taatcaacag caaaagctga tcccttctgt ttgtttaaag
    19381 ggatttgaac agtcaggcct gcactcggcc ccgtgtatac ggtggtacgt tcttcgtcgc
    19441 tggtaatacc atcttcatag gtatacgcac cacgtaactg gaggtatgac attaatgcat
    19501 actcaaggcc aacggtatat tggtccttga agaaggagtt ggaggtaaag gttccggcaa
    19561 cggtcagcct gtgcatatcg gagaaatgaa aatcgtaggc agctccgatg ttgagctgtg
    19621 aaggaagttc aaaatctgcc gaacgctgtt cgagggtaaa cttgttgtca ttatcgggaa
    19681 ggaaactgcg gatagagagg ccgtcgcctt taaaccgcat agtaggtcct atgttcttca
    19741 gcgaaatgcc gaagtgaaca ttttcctttg ctccggtcac atattgaata ccggcatcaa
    19801 gggcaatgcc ctgagcgctt gcatcgctga tcgattccga aatgatcttc aggttgaaac
    19861 ctccatagat gctgttcgaa aaggcttttg cgtatgaaag gttgatattc atgtaacttg
    19921 gcgaaaagtt accgataccg ccttcgggaa ggctggtagt ggtaatggga agatcgccga
    19981 atttcatagc catgatggct acgcccagta cgccggattc tccgagtttt tgactaagac
    20041 cgaaattgtt gatggtgatt tcggaacctt tcaaatactg ggtattgctg aaaatgagct
    20101 ctgttccttt ggtaaaggcc aggccggcaa tattgttata cattgattcc aggccgtatg
    20161 cattagctac attcacaccg ccccatcctg aactccgcgc ccatggattg attaacaatt
    20221 cggatgcccc tgattgcccg gccctgtctt tattaccggc caatagggtt acgggaagag
    20281 taactgcccc tatcaggagg aaagctatga tggccttata tatgtttttc atagtcagtc
    20341 tgttatttgt tgttattact gagtaaacct ccttaattgt taaaatgcat tcaagtcaac
    20401 gggacgaaga acaccaaacc atttaacaac tttctcgcca acgcccggca ccttaacatg
    20461 aataaggtac aggcctccgg agatcggaat accggcagca tttttcaaat cccagtcgat
    20521 ataggttgtc gacgcttctt tagccgggaa tttcctgatg agcgtacctg atgttgtata
    20581 gattgtaaca acggcatgtt caggaagatt cacgattttg accatattct tcaactggtc
    20641 ggcttcatag ggtgagtaag cgtaataagg attgggaaca acattgatct tatccaggtc
    20701 gctctcagcc ttcttggtat tatcggtaac cgttgaaacg ccttccgttg tgaaagtgta
    20761 catcgggaag aaattgttgt cgttatgggc ctgggccaaa tctcctgtct gggtaaacga
    20821 gaaattggga cgataaggtt tgccaacgcg gagtttcacg gttgcatcac agcctaacca
    20881 tgaggttgaa ggaacggcaa gcggcatgga tacccacatg gcgttcgaca tctggataac
    20941 tttataacga gcggcaagag ttcctgttag attaaactgc gtcacaaaat tcttaccggc
    21001 atcataggca gggttgtcat agtgtgaatc aaggccttga tgaaactgat cctgggtatg
    21061 cgacatgata tagatataat gcttgccgcc catcacagga ttaccttctg catccatctc
    21121 tctggatgaa gggttgaaga gcatatcatt accgttgtca ttgaccatat tggaggcctc
    21181 tccgaatacg atattaagac gttcgccggt ttcgaggttg atggcataac ccgggaacca
    21241 acccatgccg taagctgaaa tatagtcgga gttctttgcc ggatcgctgc ttacaacggc
    21301 agtatcgcca tcctggttaa ccgaccggtg tttcctcagt tcaaattgtt tggcccaatt
    21361 ttctgacaga ctttgatccg gacacatttc aaggatcggg ctgcgcgtcc atttgctctt
    21421 atcggatgtg agtacgatat caacgctggc caggtcatac atgttgctat acctttttcc
    21481 tatgtatgaa attgccccgt aagcgggtcc cagattcgat tgcatctgat catggccggc
    21541 gctcaaatta tagggcgacc acgtaccgcc gatcaggttt tcatatacct ggttcgggtc
    21601 ataggcttta ttattggcat cgaccccttt catatcccag tcgttgttta ccttattatc
    21661 cttgtcaaat acatttcctg accggatcca gttataaatc ggcgacccgt caacatcggc
    21721 tactccggaa agccaccggt ttgatgaatc ggcatagaca atttcggcgc tcaggtaacc
    21781 attgttctcg gcagccggtc cccaggtgta tttaattgta ccgtcggtat catacgattt
    21841 aaccaacctg aatgctccgg gatcaaaaat ctgggagacg gtaagcgaaa gcccaagatc
    21901 aaggaatatc tgctcgtttt tgtaactgat cagggagtct gatttatata ccctgccggt
    21961 agcgttatcg gtaagagtcc agcggccatc catcatcaaa gtgtccattt cacctacctt
    22021 aacggttgaa tagaacatcg ggtcaaattt caccgtgtac tctgcattct taacattcag
    22081 cgggtcaata accctgatat cgagaggtcc tttgcctttt tcatatttca ggttataggc
    22141 gatgggataa gtgtcggaac caagttgatt gttttcatca acagggctct tcgacatgat
    22201 ttcatccacg ctttccttcg aaatatcaag aatattgcct ccgttgccct ggccggcaat
    22261 acgggtaatc gtcacaccct gtccataatc ggctttagct tcaaccatgc ctaccgtagc
    22321 gtgaggtata ccggtatagg tcttaatgtt tttacggccc gggaggtagg gaagtttttg
    22381 gcctgtcaat aagtcggcag aattagggtc gtaagattga tacgagttgt aagcataggc
    22441 gattgccagg tagtagtact gctggtggtt tacaagtttt tcaccggtaa aggcgtcttc
    22501 ggtagcaaca aaactgtggg taataccggt attggcgccg ttaacttctt caacagggat
    22561 attaccgttg agggttgcat cgtaattgta gttgatgagc cgggaaatat catttttcac
    22621 gtcgcattga aaaacaaggc gggccttggc cggatcatga atatcggcgc tggtgatggt
    22681 agcattctta agctggaata cctggtaacc ttcgaagtta taggtagaat cccaacggtt
    22741 tgcatggccg agggtatcgg gggatacgat acggggatca tattcctgat atttttccag
    22801 gtaattattg ccggcgtcgt tggtcttgcg gttgctcagg tatatgatga gtttcctgtc
    22861 aagttcgcgg atggtgaggt caggagcgtt aggccctgaa ataaccttga agcagttatc
    22921 gaagagtatc tggcatttat cgtcggtttg ctggagcagt ttcaccgaag cgaaaggtcc
    22981 gcctgaggca gcacgcgccc agggaatacc gacggtaata taattgacgg cgccgggttc
    23041 aagggtaaaa ggacctgccg actgcataaa gcggcggtcg ttagggtcgt ttccggctgt
    23101 ttcttcagtc cagtaaggca ttccgttagg aggaagcccg ccggttcccc aatagcaggg
    23161 gtcggtattg cccgggaaca tgaaatcgca atcaggacct acgccgcctc cctgtgcatg
    23221 ggcatttccg ccccagatca tatgggtttc gtctttccag atacctttca tgaggttata
    23281 gtattcaatg gcaacatccg ggtcggtcat gtaatcagga acaccgctgt tgttgtggta
    23341 tacaaaacgg cgcatgccaa aacgctcatc atctacaatg ccgttggcaa aattcacacc
    23401 gttgatactt acgtcgcagt tttctccggt tttcggattg aacttcggat tatcaatacc
    23461 atcgggatcc atataaggcc cctggaagta gtcaacaccg attgcaggcg gctgggcccc
    23521 atatgcccag gcctgtccgt ttccgtcgac cggtttaccg ttatagcagt agccaaggcc
    23581 gcgtttcaca tcgcatccaa cgtagtcgtc gccggcaaat ccaagatcgg tatcaaccca
    23641 ctggcagaaa taggtttctt tgaggcggta agttgaacgg ttgatgattt cataactgta
    23701 aaaagtcatg ttgttcactt cgtcgttggt ggagaaaccg aatgcctgtc ccctgatttc
    23761 aacgcctata ggctcgccct cagtctcggt gtgtatattt cctttatcat tgaaaaccca
    23821 ccacagggtg tagtcgcctt tgataacctg gtcaaccagt attccgttgc cttcggcagt
    23881 aggaattttg gtatggcaca gttcattcga aacatcgtaa tatggataat ctccgtcatt
    23941 ttcgggttca tagcgaaggt ctccgtccct gtcgtagaaa ggggcaagga attcagcctg
    24001 gcctttctcg ccgtgagcag gccagttttt gatggaagcg gggatctggt atccgggata
    24061 gcttgaagga tcatcccagt atgcaaggaa ttcatcaacg tcggcgcgcg acatttcaaa
    24121 aagtttgtca tacttggcgc aggttacttc atcaatggat gcggtcccgt caacagtcag
    24181 ggggccgggc cagtagtcat tccctacctg gcggtaacgc agggcggcta gcttcaactg
    24241 attattaacg tcaagtccgc ccagccacaa tgaaccgctg aacatcgaca tttttttcga
    24301 tcctttgggt atttcatact gggctgtttc aaagtcccac cacatgtcac cgctgctatg
    24361 aatccgggta cgaacattgt ttatttccag ccatttaaaa tttgatccgg gaaggcaccc
    24421 ggcagcggtg gacttaatgg agctttttgc cgatctactc ttttcagcat gatacctttc
    24481 agcaaaggta cctgtcacca gcagtgaaaa gaataagata agaggcagta tgcgatttaa
    24541 actcttcata ttatagctta tttatgtttg ttaagcaaat cgtttttcgt atcgtgtaca
    24601 attagaaatt aaagctgagg cctaaccgga tcatcctggg cgaactgtaa ttgtagggac
    24661 tgttagcacg aagccggtag aggtcgatgt acgattcggg gttgagttgc gaataaatct
    24721 gtgcctggta ttcatcagcg gcaaggtatc cgtcatcgtc aggattgccg gtagcagccc
    24781 aaacgcccat gatgttctcg ctgttgagaa tattcaggac ctgcagatat acattaagat
    24841 acaggtctgt tacgttgcct ttggcatctt tccgtgtgcc tatgtggaaa tccttgtcaa
    24901 cacgaccatc gatcttgaat gaccagggaa ggcgcgatcc gttgatggag ccggatatga
    24961 tcgactggtt tccaaggaac gaaatcttgc ttgaacgggt gtagggagta cctgaaccgc
    25021 cgccgacggt aatgttgaaa ccggcgttct ccaggatttt ctttccttta atgaccgggc
    25081 cattatattc tttaccttca ccgtagcggt agtcaatatt aaggttgacg gcatgacgac
    25141 ggtcaaaatt aaggggaata agggtacgga ggtttggctg tcctgactgg ataagccctt
    25201 tggaggtttc ctggttcgaa ccggttccat tggcaaattg caatgtgtag ctggccctta
    25261 cacgggtgtt gccggtacgg cgcaggtcat aggtaacagt gaggcctttt acggttccga
    25321 aatctatatt attataggaa taataagttt tggggtaagc tcctgtgtag cggtatatct
    25381 gtatctggtc gcgcatttcg cgatagaatg cagaaagatt gagggatgaa gtattattga
    25441 gtttctgttg gaaaccaagt tcataatcta ccgtacgttc cggtttcagg ttaggattgc
    25501 tgaaagtcgg cgcattttcg gtttcccaga ataagtaact gatcgggttg aaagtactgt
    25561 tcgatttggg cctttgggtt agcacatcgt agtgggcata gaaaagggct tcgtcggaga
    25621 tggggaacga aaacgaaata cgcggcatca ccgtaatctg tggctcgtaa tcggtaaaag
    25681 atttcccggt tatactctta tcatcaggat cggtcaggta gggcgaaatt gatccgcctg
    25741 atacaaggcc gccgttgctg atgtctaacg atggatcggt tactacgtta ccctgggcgt
    25801 cgtaccagtt cattccgtca cgatagccgg ttatgcgggt gggggaggta acattgtcaa
    25861 cataaacaac gtaatcggag cccattcctg aaggatgagc gccgaattca gttacttcgc
    25921 cgacagtctt tgcagggtac agaaggtacg ggtcctttaa tacgctttgg tttgcatcgt
    25981 aacggtcaac acgtaaaccg atattgaaaa ccaggtcttc gaacgaaaac ttatcctgga
    26041 tataacctgc catgtaaatg ggttcaaacg gtgcaatcag gcgggtatag ttgccgtcgg
    26101 catcagtggc attgtaaaaa tcatcgatgg tcggcttgga agtgagttta ttcccgtaat
    26161 agtcataacc gcgggcagca gcaacctggt tgccgctgtt caggagttcg tcggcgctga
    26221 aaagattgac attcagtcca ctattaaggg tcatggtatg catcttgttg tcggcatcgt
    26281 aatagttgat ggtctgttta tcgatatcgt aagagttgat atcaatccag tcggttccgt
    26341 caacagctaa tcccatgagc cggcgcaggt tcctgtcgaa agcgcgctgc gatgaagcgt
    26401 tatatttcct gtggtaataa gtagtgtcga aaccgccttc atgataaacg gttgtggggt
    26461 ctgacatgtc aagctgtgta atatgggagt ttgcaagctg gtacatgagc gtccagaaat
    26521 taataggagc atagctataa ctgcttgact tggattgttc gaaaacaaag ccgagcttta
    26581 tttcatggtt ccccacatcg gctgaactgc tgaagttaag ggttacctga tcaacatccg
    26641 ctacggcata acccgactgt aatacgccgg gtgatgcata catcccgtaa atatcggaag
    26701 gtgaatcacc gttgagcatg cctccccctg aatagatatt gtccagttta tcgtagtgac
    26761 cggcgctggt attatataag gagtagtagt aggaggtgta gttggcaagt acagggttgt
    26821 attctgcacg gtcgaagtta acacgcgtat cgttgtatgc gttcatcaca tatccgttga
    26881 atccggcggt ggtggtatca agctcaaacg accgggtctt ataagtggta aatttaccaa
    26941 catacccgta ggcaaagaga ttatccttaa aatcaggatc ataggttttg gtatggtccc
    27001 tggtgaagtc gccctggatg gtatagtaaa cgttcttgat gggcgaccgg ctttccttat
    27061 ctgaagggaa acgctgggtg aaccggccga ataacctcca ggtagtctga tcggtataag
    27121 agtaacggtt gtaattggca agcgattgaa cccagttgaa attgtggccg ctgttggcat
    27181 tgtagctgcc gcccagggtc aggttaatat tcgggccggt tttgatatca aactttcccg
    27241 aagtatttat cgaatagccg tcgttgttaa ggtttgtttt gatatgttca aggtcgctcc
    27301 tacggataaa ctcggcattt tcataagtac cgaagccggt tcccgacggg cgcaatggat
    27361 tcttttcgag gtaggataga acatcgtcct ttatcttgta attaccgata gccaggggat
    27421 cctgatcttt cttgtattcg aaatcggcag caaggaaata ccctactacc gaactggttt
    27481 tatctttatt ccagaaaaga ggcccggtaa gatttacccc gcccctgtta taaccataag
    27541 gatcgaggaa ttcggaggtt tcgagttcga tgcctccgcc aaaggtacgt gaaggtcctt
    27601 tggtggtaac attaataata cctcccgtag catcaccgta ctgagcagga ataccgccga
    27661 ggacaacgga tacctgttca atggccgact ggggcagggt gctgagaccg cgcaccttga
    27721 taccgtcgat gtaggtaacg ttaccatcgg tacgttggcc gcgcatgctg ccaacttcgc
    27781 catcggctga aaagacaccg cctacggttg caacaataga tccgacttca cgcgaaggca
    27841 tcttactgat ttcttctgcc gttactgttc cgccaaccgt ggttttatcc ttatcaataa
    27901 gcggaatctt gtaatctaca acttcgtaag tcgtgatctc gatggcggtg gattccatct
    27961 caatgttaaa gggcgtgatc tggtcggctt ttataagcac tccctggatc aacaccggct
    28021 tataaccgac ataagtggcc ttgatgttgt acttaccggg cgacaacggc tttatcgtat
    28081 aattgccgtc gaaatccgat gtagaaccac cggcctgggt tccgtttgct tctgcaatga
    28141 tgttggcgaa tgggacaggt tccttggtgg ttttatccag gatcttccct ttcagcgttc
    28201 ccgattgtgc aaacactgca atgtttgcag aaagaagcaa aacaaagaca gtgagtaatt
    28261 ttcttaccat agaatgtggt tttgtgtttg ctattacgaa aggttattaa gtttctctgc
    28321 tagagtaagt atgattttga caatataaat tccccctgaa aattattcag gctgctaaat
    28381 taaaaaaaat taagatttcg ttaacatttt aaaaataaag ttaacagaac gaaatgattt
    28441 ataactgata ctgctttccg cgtttaaaag ttccgtatat caaagtacag aaatagaccg
    28501 tgttcaggac ccggtccgcg atcgttcagg aacacttcgg ccctttgtta taaggctttt
    28561 tcatatacct gttgaacttt ttcgcatgtt tactggtttt tttcatcatt ttccgggctt
    28621 ccttgctttg atttttcagg tgcatatcca atgccttttc atactctgtt tgcgcctttt
    28681 gggcttcctt tgcctgtttt cgttctactt tttcacgggt gccgggcctg aggaggctgc
    28741 acgaagtgtt aaacaaggcg aaacttacta tgaaggcagt caggagccat ttcggccagc
    28801 gcttatttga acggaaaacc ggcatgaaat atctgtaata tttattcgtt aatcaggttg
    28861 cgaaggtaat tcattcctaa ttttacaacg aaataaatca attactgttg cgcataacga
    28921 taatatattc tgccatagca ttgcttttag caatactgcc gttctcctgt accaaagagg
    28981 gaacctccaa gataccctat gtgtatgttg attactacct ttatcctaat acactggatt
    29041 atattccgca gggcggcacg ataatctatg atggcggcta ccgggggatt ataatatacc
    29101 gtgcttcgga aagcgagttc atggtttatg agcgttgctg tccgtatgac ccggaaaatg
    29161 caaatgcgta tgtagaaccg gaacccggtg ggcttacggt aattgattcc gtttgcatgt
    29221 cgggatatat actaaccgac gggtcgccgt ataaagggcc gtcaaatgtt gccctgcgtc
    29281 agtaccatta ttcgtataac ggcgaagtgc tccatatcta taattaaaaa gcctcccagg
    29341 tttatatcac ccgaaaggct ttttatcgta ttctttcaaa aggttttctt tatccctttt
    29401 gcgtggctgt tggccgttga cttttagctt ttagctgttg gctgttggct gttggccgtt
    29461 ggttttgagt tctgagttct gcgttttccg ttttcccctg cctgccacct ccaggctcgc
    29521 gcaaggcagg caggcctgcc tacctctcac acaaagccat acacctgcag gtaaagtttt
    29581 ccgttctccg ttctccgttt tccattgccc tttgactttt gccctttgcc ctttgccctt
    29641 tgacttgccg gcttttggcc tgccatctta cgcactgcca gcccaggcag gcaggctctt
    29701 agccgttagc ccttagcctt tggcgttttg agttctgagt tttgagttct gagttctgag
    29761 ttttgagttc tgagttttga gttttgagtt ctgagttctg agtatcccct gcccactccg
    29821 tcctccgttc tcccctgccg gccacctgca ggctcgcgca aggcctgcct gcctgcctct
    29881 cacacaaagc catacaccgg caggtaaagt tctccgtt
//
LOCUS       DAOPYV010000036        29891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_1514_length_29891_cov_12.672040, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000036 DAOPYV010000000
VERSION     DAOPYV010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29891
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1514_length_29891_cov_12.672040"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(442..3051)
                     /locus_tag="PKI35_06355"
     CDS             complement(442..3051)
                     /locus_tag="PKI35_06355"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNX44043.1"
                     /translation="MSKGFFFDFFTDKNQTLYFYNWQRELFSLDKNNSVSKEILLEKL
                     NIKLPIVGTDYKRIKSLYVTKNSKDKWTIWTNVGIFIEDIPTKKIFYVDSDASCLKIF
                     PAGHNSTYYIHMRDNNITKVTNRFFKEIIHYPGNERKIMNIMEDSDGYLWIAAYDEGV
                     YCFKRNNKVLLHLNIKNALGLFQDHEKNIWVSSESNGIYLINHDMMTQKHYDRSNFDD
                     CGVHLLSDFPGTGVWCTNTKSVFLLHNESFFRLSVPEELQPINLLHYLRNQNLFLGMK
                     STRACGYSEMKLVSNSKSIQYERKKKYPDLQAKKIFNDHTGVYTVLFEQDRILVTDAG
                     LPTTTHGYKCVGERINNAFYDTGNQLIINARKNYLFKNDKLIPYPELSRFDGTVITDH
                     LVLNDNVELYSIDGDSLYLLKNHKFYNLTEAFNTPIALPIEKILYDDTTLYVSTLKDI
                     FVCHNPLNAISGNPVQLEPLNIGFNNINDILIHNDSLYIASDDGLTIISEASITKSNA
                     LPPIPYTQSVMVNDEEYNLSGEGIKLTGKNKIQLTFGCISFFSSSLFYSYLLEGAETK
                     WTVGTGHEMKLMYQNLPRGHYIFKIRVRKSNSDWSKPLEIPIIIKPTLLENPIFWAIV
                     CLVAAGIITLILVTIRARKMKKVEVDHQLIVMEQKALQSMMNPHFIFNSLGSIQNYLL
                     TNKGTEAIIYLSNFARLIRQNLNAINTPMILLEEEIDRLNNYLNLEKQRLENKFDYHI
                     EIDDSIEDDQVYIPSMMIQPFAENSIWHGIATLEEKGVISICFYANNEKSMRIIIEDN
                     GIGMKKAQEYSVRGSHHQQLGMQIIRKRLDLLSKKYHTETKIAYSDCHTGLDNPGTRI
                     EIIIPILNSTADF"
     gene            complement(3416..4210)
                     /locus_tag="PKI35_06360"
     CDS             complement(3416..4210)
                     /locus_tag="PKI35_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX44044.1"
                     /translation="MKKYNAIIIDDEKNIREALTILLKQYCPEIQVCGIACSAGEGRM
                     LLETNMVDFIFLDIAMPKEDGFTFLRSIPSHKYGIIFATAYQEHALRALKANAIDYLL
                     KPINPIELQAAVKKAIYYHELRNGYSEFQEVYQQSLNNLTENLSSRSNPVTKLTIPEQ
                     SGFRLVNVDEVMYLQAADNYTVFSLTDKREIISTRTLGEFEKILESPEFFRIHKSTII
                     NLNFLTGYSCVQGNYAELMDGSRLDISRRKLSEFRDRVKNYSFSID"
     gene            complement(4334..5851)
                     /locus_tag="PKI35_06365"
     CDS             complement(4334..5851)
                     /locus_tag="PKI35_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006281404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HNX44045.1"
                     /translation="MNSQIGFDNEKYIEEQSNEILNRVKRFNDKLYLEFGGKIMYDYH
                     AARVLPGFDPNVKMRLLQKLKDKLEVIMCIYAGDIERKKIRADFGITYETDTLKTIDD
                     FIDWGIEVKNVVITRYTGQYAATVYKNKLERRGIKVTTHNPTAGYPADIDRIVSDEGY
                     GANKYIKTSRPIVIVTAPGPGSGKLATCLCQLYHEYKRGVKAGYAKFETFPIWNLPLS
                     HKVNVAYEAATVDLNDKNLIDPHHFEAYNETTVNYNRDIEAFPLLRKIIEKITGEPSF
                     YKSPTDMGVNKAGFGILDHGFVQQAAHQEIIRRYLKCACDYATGLMDEDTLHRSEMIM
                     KRVDAKVEDRHVVEPARKAARDAELNGKGNDGMYCGAAIELKDGTIITGKNSPLMHAG
                     SSLILNAAKQLAGLPDQLQLIPENVIASIAYLKKEVLRRKKISLDVDETLTTLGISAI
                     SNPAAQMAVDKLKELRGCEVHLTHLPANGDEAGFRKLGVNLTTDPNFSSKSLFVY"
     gene            6119..7423
                     /locus_tag="PKI35_06370"
     CDS             6119..7423
                     /locus_tag="PKI35_06370"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX44046.1"
                     /translation="MSVIGLIPKDIRAIKGVFSSLRSRNYRIYFIGQGVSLIGTWMQN
                     IALSWLVYRLTGSVFLLGLIGFTSQIPTFILSPFTGVVTDRHNRLNIMKLTQLFFMLH
                     ALTMTLLVMFNLVQVWHIITLSVIFGIITAFDAPARQSLVIDLIDDPGNLGNAIALNS
                     AIFNAARLVGPAIAGIVIAIAGEGICFLFNTLSFAAVIFALMQIKISFRKVVGEPVNF
                     KESFREGFDYTFRTMPIRTLIIMLALLSVVGLSYIVLLPAYAKENMHGSSDTLGYLMS
                     AMGAGALSGALYMAARKSVLGLAKVISTSVMVMGAAISLASFSGNMFLSVVALFFGGL
                     TMVLSLSSINTMLQTLADEDKRGRVMSFYAMALMGTTPIGNLLAGSIASGIGISWTLL
                     MSGVITVISGIWFRMKRKSLRKHVRPIYVTKGILPGLPNDLT"
     gene            complement(7601..10843)
                     /locus_tag="PKI35_06375"
     CDS             complement(7601..10843)
                     /locus_tag="PKI35_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNX44047.1"
                     /translation="MKQKILSLVILFLFGLGCNAQQYFFSGYSIADGLSQSVVTCILQ
                     DSKGYLWFGTQNGLNKFNGYTFDIYTYNPDDSTSISNNWIYSIDEDRDGNLWIGTKGG
                     LNKYVRNEKRFDRIRYTFSRFPDISKYVYGVKCARNGKILINTPPVLTICDPATMTFR
                     HFAAPLDYDGGVKDYNIPLLEDADGLIWLGSARGLARFDPGSSKFKVFRNNLSDPSTI
                     SDDNITALFQDRKGNIWIGTSKGLNRFNKTGKLFDRYFKDAKNPNSVSNDFIRAIIED
                     HTGSLWIATEGGGLTRMINGKNGKLGFEAYSAEKNSLSHNIILSLAIDRSENLWVGTL
                     SGINKTDLKKQKFRLYRKSDSPYSVDLSGNVIASLYKDDSDNIWIGTWGQGLNIYNRK
                     TGKVEHFSSRLKGRNFISNDFVHTIFEDSGQHVWLGTRDGLLIFHKESHRFVRPGRFS
                     GNPGLPDLRGLRIFMMMQGHDGDFWIATQDGLYRKKHAGTELERFHVGALPEHRISAN
                     LVYSVLEDREGLIWIGTIDGLDVFNPKTSQMKHYTKTEGKNSLADNFITVLCEDHNAD
                     IWIGTSSYVNKYSKKSNIFEFYSKEQGLPSNLVYSILEDKNKCLWFATGNGLCRFDST
                     GNGFRVYTVEEGLQSPEFNLRASYLSKDGEVFFGGMNGFNSFYPDSLLNNPYLPSVVF
                     TSAYKAKKGLNEFLDFEKSNKIVLNHNDYSFTIEFSALEFTNSAKNRYKYRLESIDDD
                     WIDIGTRNFVAFSNLPPGEYVLRVKGCNNDGLWNEAGSNLTIIIRPPWWRSTAAYIGY
                     FLLVILSVFFIVKLRERHHVRDRKLLEAKVRERTLQVEEQKLEIINKNIELNELNASK
                     DKFFSIIAHDLRNPFNAIIGLTDILLLDIPYQDTRKIQKTLENIKGSSQQAHELLENL
                     LLWARSQTGTMAFDPEPVELKTLLEKSIELVGVQAVRKNIEIHTDFKDCCNARVDVNM
                     INTVLRNLLTNALKFTPQNGAVHVGLSMNKGFCILSVRDNGIGIAREKLGTLFSIDTS
                     HKTKGTNQEPGTGLGLILCKEFVEKHGGRIEVKSEPGEGSEFRVFLPAGL"
     gene            complement(11185..12753)
                     /locus_tag="PKI35_06380"
     CDS             complement(11185..12753)
                     /locus_tag="PKI35_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNX44048.1"
                     /translation="MKHLSLLAVLAIPLFFTTSLQAQHDRITDKIIETGKTDNQTMDH
                     LNVLCNRIGGRPIGSDAYENAAEWAASKFREWGMKVEMDEAGTLPVGFNRGPWSGRML
                     GSDGMTLHFATPSYTAGTKGVQTGHVVLEPKTQAEFDRMKGKLKGAWVLISGESNGFP
                     VDISKAADAKRAKVIAENALIEKKNDSIRGLNWKNHDNPKIDLLPYKEEPALFYRQMA
                     EAGILGIIQSAPVPIRALYDRNNIDSMTFENLPAIPDIKLDEHQYKVIEQMTEERQSF
                     ELEFDIRNYFKMGPIKYCNVIGVIPGTKYPDEYVIMGGHLDSYDVATGTVDDGSGIAP
                     AMEAARLIMKSGAKPKRTILVCLWAGEEFGLLGSESWVKRNEDKWNKISNMFNRDGGP
                     TVAKSLSVPEAMMEDFENICKPLNSINPEFPFTLKKMEPMELPKKAWGTDSGPFAVRG
                     IPVITFGCEDPKGYDFNYGEIWHTERDLFNKAIPEYLNHTSILTAIVVLGVADLSHLL
                     PREGYYLPPKEETK"
     gene            complement(12905..13933)
                     /gene="galE"
                     /locus_tag="PKI35_06385"
     CDS             complement(12905..13933)
                     /gene="galE"
                     /locus_tag="PKI35_06385"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNX44049.1"
                     /translation="MMGKILVTGGVGYIGSHTVVELQQSGFQVIIVDDLSNSMIEVLD
                     NIAAITGTKPEFEQFDLADSSLTADFFSRHPDISGIIHFAAYKAVGESMEKPLDYYRN
                     NLLSLINLLEGMKTHRIGNIVFSSSCTVYGQPDELPVTESAPMKPAFSAYGNTKQMCE
                     DILKFTVNAHPLHAIALRYFNPIGAHPSALIGELPLGIPNNLIPYITQTAIGKRDYLR
                     VFGNDYPTPDGTAIRDYIHVVDLAKAHVIALKRMMEGRSRSNPEVFNLGTGNGFSVMQ
                     IIEAFERVSGLKLNYKIVGRRAGDIEQVWADTRFANEELGWKAEKNLDEMMASAWKWE
                     LALAKKDA"
     gene            14174..15508
                     /locus_tag="PKI35_06390"
     CDS             14174..15508
                     /locus_tag="PKI35_06390"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNX44050.1"
                     /translation="MKIAVVGSGYVGLVTGTCFSEVGIDVVCVDIDQKKVDNLKNGIV
                     PIYEPGLEEMLHRNMKKGRLNFTTSVAEALIGSEVVFIAVGTPPDEDGSADLRYVLAV
                     ARECGQRMNDYLLVVTKSTVPVGTASKVRAALQEELDKRGVSIPFDVASNPEFLKEGA
                     AIEDFLKPDRIVVGLDSPRAEELMKSLYKPFTLNGHPVIFMDITSAEMTKYAANSMLA
                     TKISFMNDIANLCEIVGADINMVRKGIGSDSRIGTKFIYPGIGYGGSCFPKDVKALIS
                     TAGDYNYELRVLKAVEAVNNDQKSVLFNKIMKFFNGDIKGKTVAMWGLSFKPQTDDMR
                     EAPSLVIIRKLLEAGAIVKAYDPVAIAEAKHHLGDSIIYADEQYDALVDADCLALVTE
                     WPEFKFPNFRIIRKLLNFPVVFDGRNIYDKDEMKRNGFEYFCIGVNTENSQE"
     gene            15558..16499
                     /locus_tag="PKI35_06395"
     CDS             15558..16499
                     /locus_tag="PKI35_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX44051.1"
                     /translation="MIRKKVLVTGGAGFVGSHLCERLLNDGNEVVCLDNYFTGQKQNV
                     IHLLNNPYFELIRHDVTMPFFIEVDEIYNLACPASPIHYQYNAIKTIKTSVMGAINML
                     GLAKRIRAKVLQASTSEVYGDPQVHPQVESYWGHVNPIGERSCYDEGKRSAETLFVSY
                     HKQNNVKIKIIRIFNTYGPRMHPNDGRVVSNFIVQALQNKDITIYGDGKQTRSFQYVD
                     DLIEGMVRMMATRDEFTGPVNIGNPNEFTILELAEKVIKLTGSKSKIVRMPLPPDDPT
                     QRQPDISLAKKELHWEPKIQLDEGLTKTIDYFRSIIV"
     gene            16635..17522
                     /locus_tag="PKI35_06400"
     CDS             16635..17522
                     /locus_tag="PKI35_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNX44052.1"
                     /translation="MKHADFIYPTDSTKGLKDFNPSFSGKYKSKKSAEKDLEKGIEQL
                     SVMQDMLYAHDRYAVLIIFQAMDAAGKDGTVKHVMSGINPQGCQVTSFKVPSAEELDH
                     DYLWRCVKQLPERGRIGIFNRSYYEEVLVTRVHPQILQNEKLPGLPKAPENDRKFWKQ
                     RFDDINNFEKFLTNQGTIILKFFLHVSPEEQKKRLLERIDDPSKNWKFSTGDVNERAL
                     WPKYMEAYEEILKHTSTEVAPWYVIPADKKWFMRAAVCDVIVERMQQLKLHYPLLNDT
                     QKADLLKAKEMLENENTEK"
     gene            17572..19137
                     /locus_tag="PKI35_06405"
     CDS             17572..19137
                     /locus_tag="PKI35_06405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX44053.1"
                     /translation="MLDHHPSLNDINNSMGLKSSKYYNQKKRWKFFLLFAAVIIVVAS
                     LWFSNLLVHKIAQDERAKIQTWANAINHRLALVNSTSRFFEQIRDEERKRVELYAAAQ
                     KRAAEAANDDAFLSFYYQIIENNTSIPVILTDEDGNINTTRNIELDTEKIKKITPEIK
                     KKLSLYPPVVIDYYQGNLNYLYYSDSKLFTEVQKVLDEIINSFFREVVDNSAAVPVVI
                     TDSTCRHVIASGNLDADRLTDSVYLKQLIAEMVSENDPIVITVADRGKNYIFYKDSYT
                     LTQLRYLPYILFFIISIFLFVAYLLFSTARRSEQNQVWVGLAKETAHQLGTPLSSMMA
                     WIDYLETKGIDSDTIGELQKDVNRLQTVTERFSKIGSTPVLKPENVVELVYNAVAYLK
                     TRTSQKVQFSINVTDDKVIMAPINYHLFDWVIENLVKNAVDAMSGKGKIKIDLYEDDL
                     FVKIDVQDTGKGVARKMFKTIFNPGFSSKQRGWGLGLSLSKRIISEYHKGKIFVKEST
                     LGKGTTFRIVLKK"
     gene            19228..20358
                     /gene="hemW"
                     /locus_tag="PKI35_06410"
     CDS             19228..20358
                     /gene="hemW"
                     /locus_tag="PKI35_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNX44054.1"
                     /translation="MAGIYIHIPYCRQKCSYCNFYSLATTKYRERLIPALVNEMQLQR
                     DYLEGEIVQTIYFGGGTPSLMRAGEINRVLSAVCNIFGVDSQAEITLEANPDDLRAPK
                     LHALKQAGINRLSIGIQSFEDADLRFLGRSHSAAQARNCIDRAQNAGFSNLSIDLIYG
                     IPTLDSESWESNLLTALSCGIPHISAYALTVEEKTPMALKIKRGSIPDVDDEVQLQHF
                     GILRSLLQAHGYQHYEISNFCVPGMFSKHNTSYWQGKKYLGIGPSAHSFNWVSRQWNI
                     AGIGPYLESVNKGIVPFEMEMLTKAQRFNEYVMTSLRTMWGCDLKEIEKMFGESWAFQ
                     ALADARQFLENKMMVRKDGVLYLTNDGLFHADGIASEFFRVE"
     gene            complement(20535..21611)
                     /locus_tag="PKI35_06415"
     CDS             complement(20535..21611)
                     /locus_tag="PKI35_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44055.1"
                     /translation="MKTKKNLILYILLWPLLAAVLTVALLLVFISVSDFRPKSIEPVK
                     VKGDSFVIPLPADSSLTFYTWNIGYCGLGEQMDFFYEGGRRVRPSREEYQRYRDGVIY
                     QMTTLFNKPDFILLQEVDSLAKRSYRDNQIQRIEAGFPSYSAAFAVNYNVKFVPAPVF
                     RPMGKVIAGQLTLSRFRPVSAERHAYPSCYSWPKNLFMLDRCFILSRYNVSNGRQLVL
                     INLHNSAFNDAADMRIQELGMLKKLMKAEFDKGNYVIAGGDWNQNPLPYDSSAVSKGY
                     DARCIHPGIPEHALPENWQWAYDSFLPTNRDVRTPYRTGVTPVTLIDFFVLSPNIELK
                     GVKTIETGFRFSDHQPVGMAVGLR"
     gene            21758..23296
                     /locus_tag="PKI35_06420"
     CDS             21758..23296
                     /locus_tag="PKI35_06420"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNX44056.1"
                     /translation="MPILGSIIKSALEVRNRIPVEKLKPQDPYNVQVRTLRKLLRTAQ
                     LTFFGEEYGFSQMLHSDDVVDVFQQKVPLHDYNSMHRRWWYRTLNGEAFVCWPGQVKH
                     FALSSGTSEASSKYIPVTPDMLRAIKKTSIKQLLSLTKYDFPPEFYEKGALMLGGSTH
                     LQYNGTYYEGDLSGITTGNIPFWFQHFYKPGNRISKERNWSTKLEEIVKKAPEWDIGA
                     IVGVPAWLQILLEKIIDQYNLNTIHDIWPNLNIYVHGGVSFSPYVKGFEKLLAHPLTY
                     VETYLASEGFIAYQSRPGSNSMQLVLDNGIFFEFVPFTDENFEEDGTLKTTAEALTID
                     EVEEGREYALLLSSCAGAWRYLIGDTIKFTSKALSEIVITGRTKHYLSLCGEHLSQEN
                     MNRAIEMLEHELNISIREFTVAGIRHGSMFAHKWYLGTNDKVDTEVARRKIDEYLKIL
                     NDDYRTERLHAVRDVMVEILDPEVFYNWMRLQGKEGGSFKFPRVLKNAKYEQWDEYLS
                     SLKK"
     gene            23409..24032
                     /locus_tag="PKI35_06425"
     CDS             23409..24032
                     /locus_tag="PKI35_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNX44057.1"
                     /translation="MHPLIQGMILGLTLAALLGPALFTLLQTSIHRGWRSGVFLAIGI
                     IVSDFSVLMLAYLGALQLINKRNNYLIAGIIGGIILILFGIYTFNRKIHFDENNNPVE
                     TKVAGPLTYILKGYFLNIMNPFVWFFWISSMVGVSSNFGDDKHSIMIFFIGTLSITFS
                     TDILKVFAASKLKRHLNGKILLFINHSVGIILMLFGVFLMIRTVVQF"
     gene            complement(24310..24705)
                     /locus_tag="PKI35_06430"
     CDS             complement(24310..24705)
                     /locus_tag="PKI35_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNX44058.1"
                     /translation="MESEHPRIDKWLWEVRLFKTRSQATEACKAGKVKIENISVKPAK
                     EIKTGEIIIVSLNPLFKTVRVKDFPKSRLSAKLVPDYLEDLTPQEEYDRVKLIQETNS
                     EYRDRGLGRPTKKQRRLIDKLKSSKDQWK"
     gene            complement(24720..26024)
                     /gene="der"
                     /locus_tag="PKI35_06435"
     CDS             complement(24720..26024)
                     /gene="der"
                     /locus_tag="PKI35_06435"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNX44059.1"
                     /translation="MGNILAIVGRPNVGKSTLFNRLTGSRAAIVDPTSGVTRDRHYGH
                     SDWNGIEFSVIDTGGVVIGGDDIFEQAITRQVELAIEEADIILFMVDAREGLSPLDED
                     VAELIRRSRKKAFLAVNKIDTPDKVNEAAEFYSLGLDGVFPVSSANGSGTGDLLDEIV
                     ASFRKTETEEVPDLPKFAVVGRPNVGKSSLVNALLGKDRHIVTPIPGTTRDSIFTRYN
                     SFGFDFLLVDTAGLRKKGKVYENIEFYSVMRSIRAIENSEVCLLLIDATQGFEAQDMN
                     IFSLIEKNHKGVVMVVNKWDLVEKDTNSTKHFEEYIRKQTAPFTDYPIVFTSVLTKQR
                     IHKALELAVKVYENRKRRIATRKLNDSLLPVFTETPPPLYKGKDVKIKYITQIKTVYP
                     SFVFFCNLPQYIRDPYKRFAENRLREMFEFTGASMEVYFRKK"
     gene            26309..26707
                     /locus_tag="PKI35_06440"
     CDS             26309..26707
                     /locus_tag="PKI35_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5797 family protein"
                     /protein_id="HNX44060.1"
                     /translation="MRSSEYLQNDNFDAGICRIVSKRGVTFTVFKNAIRLNSELREKI
                     HDVIDLHLSEYAFRNNAEIIQAAREKAENESTELMMKFLREGGNYKAIEAKLHRNRII
                     KLLAQKDKKEQAELIKKYTYDTRASFCGCI"
     gene            complement(27093..27371)
                     /locus_tag="PKI35_06445"
     CDS             complement(27093..27371)
                     /locus_tag="PKI35_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44061.1"
                     /translation="MKRYFILLAILFAGSSSFAGTGNANDGILFAMAVILIMMLILGI
                     GYFTRMLNRMIRAVRTWNWIHKHTAGSPDKKQADDFHFDMTINYIGQV"
     gene            27491..28186
                     /locus_tag="PKI35_06450"
     CDS             27491..28186
                     /locus_tag="PKI35_06450"
                     /inference="COORDINATES: protein motif:HMM:NF027590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4918 family protein"
                     /protein_id="HNX44062.1"
                     /translation="MKSFADKIIDFNGSLDFKGTLPAGISLMNPFRENSEILSVSSAF
                     YRKYYSDINPRHLILGINPGRFGAGVTGIPFTDTRRLADECGINYTGRQTYETSSVFV
                     YEVIKAYGGPEKFYGDFYISAVCPLGFTIHDAKGSEKNYNYYDSKELTDAVYPFMVES
                     LRMQIGFGIKTDVGFCLGTGKNEKFLRSLNDKYKFFGEIIALEHPRFVMQYKARSKQQ
                     FIDKYLNAFTNVK"
     gene            28448..>29891
                     /locus_tag="PKI35_06455"
     CDS             28448..>29891
                     /locus_tag="PKI35_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44063.1"
                     /translation="MFTFVISSLKSYGEGTKQLEPDGPASMTNGGLGLILYDGGNTTY
                     GYRIPFAMAGCDSLYRLNVYIANPATEKIYFGFRKGSYNQLYYQFRGPNGDVVPGYSF
                     NPSPVAGEPGFIDDWQAAVNGPLTGYTPLILDPSTVGLPGNYYIEFAGSATGGGIGND
                     GVTINLIDISVVASGGTIIDGRVWSYAWQFSNQINGTSHAQNSDFYFYSNDGLTTKCN
                     INSWGGGTFYVYCNQWGVYNTDNWENDRRSVDYTYGTAPLPLPQYREFLNPPDVNVYP
                     TGTIGQICSGSLGSISYCDGSVDFIMKVTKTGIVTLNITTPSGSAIVQGEVTGYADCS
                     TFDTIHWDGMINGTPVQNGASLSMSIQYLNGLTNLPCNDIETCNDGIKVDLVRPESAA
                     GKLGIMWDDSNIPVPNAGVNSAYPGCIYPSSPAIDGCHSFSGDKRIINSWWYYLTTGN
                     ESLNIVTRNTPATPTVSPTGPSPVCQGQTGA"
BASE COUNT         8025 a         6569 c         6859 g         8438 t
ORIGIN      
        1 ccctttggcg acccgcattt ttttaatgat taatgataca cggaaggttg tggtctcata
       61 gggccagact ggtatttatc tcaaatagca ggaaatgaaa tataatacaa catgaaactt
      121 aaaataataa cagccgcccg ttaacggacg gctgtaaaat ggaaactagc cttaacctgc
      181 ttacatggaa gttaaaacag gctttaaata gtaatgattt ggaaaattaa actcatcaca
      241 caaattggtc gtacaataaa tgaattgtca ctcataagac gaagcaaata taatagtgta
      301 acattccttc acgcctggca attgagtgaa cggtatcaga aattgagtga aatgacattt
      361 tttgaaaaga acggtcatgc tgaaggggta tcagggcata aaaaaggcag ctcaggctcc
      421 ttttatatga agaattgata atcagaaatc agccgtactg tttaaaatgg gaataatgat
      481 ttcgatcctg gtacccgggt tatctaaacc cgtatgacag tcagaataag ctatttttgt
      541 ttctgtatga tactttttgc tgagcagatc caagcgtttt ctgattatct gcatgccgag
      601 ttgctgatga tgagaacccc tgacagaata ttcctgtgct tttttcatcc cgattccgtt
      661 gtcttcaatg atgatcctca ttgatttttc gttgtttgca taaaaacaga tactgatgac
      721 gcctttttcc tccagcgtag caatcccatg ccatattgag ttttcagcaa acggctgaat
      781 catcatgctg ggaatgtaaa cctgatcatc ttcaatggaa tcatcaattt cgatatggta
      841 atcaaatttg ttttcaagac gttgtttttc aaggtttaaa taattgttga gccggtcaat
      901 ctcttcctcc agcagaatca tgggggtatt aattgcgttc aggttttgcc ggattagcct
      961 ggcaaagttc gaaagataaa ttatggcttc agttcctttg tttgtaagaa ggtagttttg
     1021 aatggagccc agtgaattga aaatgaagtg ggggttcatc atcgattgca gcgctttttg
     1081 ttccataaca atgagttgat ggtcgacttc cacctttttc attttccgtg ctcttatcgt
     1141 gacaaggatc agggtgatta tcccggctgc tacaagacat actattgccc agaatatcgg
     1201 gttttcaagc agggtcggct ttataataat aggaatttcc agaggcttgc tccaatcgga
     1261 gtttgacttt ctcactctga ttttaaatat ataatgcccc ctgggaagat tttggtacat
     1321 aagtttcatt tcatggccgg tcccgactgt ccatttggtt tcggctcctt ccagcaaata
     1381 cgaataaaag agggaacttg agaaaaaact gatgcatccg aaagtgagtt gtattttatt
     1441 ttttccggtc agtttgattc cctcaccgga aaggttgtat tcctcatcat tgaccattac
     1501 cgattgtgtg tatggaatgg ggggaagcgc attacttttg gtgatggatg cttcagagat
     1561 gattgtgagg ccatcatcgc ttgctatata cagcgaatcg ttatgaatca ggatatcgtt
     1621 aatgttgttg aagccaatgt tcaggggttc cagttgaaca ggatttccgg aaattgcatt
     1681 taatgggtta tgacatacaa atatgtcctt cagggtagat acatacaagg ttgtatcatc
     1741 ataaagaatt ttttcaatag gtaaagcgat gggggtatta aaggcttcag tcaggttata
     1801 gaatttatgg tttttcagaa ggtataaact atcgccgtca atgctataga gttcaacatt
     1861 atcatttagc accaggtggt ctgtaataac ggtaccatca aaccgggata attcggggta
     1921 cgggattaat ttatcgtttt tgaaaagata attctttctg gcgttaatta tgagttgatt
     1981 tccggtatca tagaaagcat tgtttatcct ttctccgaca catttataac catgagtagt
     2041 agtaggcaaa ccagcatcgg tcaccagtat ccgatcctgt tcaaatagta cggtatatac
     2101 tcctgtatgg tcattaaaga ttttttttgc ctgaagatcc ggatactttt tttttctctc
     2161 atactgaata ctcttagagt tggatacaag tttcatttca gaatatccgc aagcccttgt
     2221 gcttttcatt cccagaaaca gattttgatt tctcaagtaa tgtaacagat tgataggctg
     2281 taattcttcc ggtacagata atctgaaaaa ggactcgtta tgtaataaaa atactgattt
     2341 tgtattagtg caccagacac ccgttcctgg gaaatcagaa agcaggtgaa caccacaatc
     2401 gtcaaaattg ctacgatcgt agtgtttttg agtcatcata tcatgattaa ttaaataaat
     2461 gccattagat tcagaactaa cccagatgtt tttctcatgg tcctgaaata gtcccagggc
     2521 atttttaata tttaaatgca agagaacttt attatttctt ttaaaacaat aaacaccttc
     2581 atcataggca gcgatccaaa gataaccgtc tgaatcctcc ataatattca ttatttttct
     2641 ttcattacca ggataatgaa taatctcttt aaaaaaacga ttcgtaactt tggtgatatt
     2701 gttatccctc atatgaatat aataagtact gttatggccg gccgggaaaa ttttcagaca
     2761 acttgcatcg gagtcaacat aaaatatctt tttagttgga atatcttcaa taaaaatgcc
     2821 gacattggtc cagatagtcc atttatcttt agaattcttg gttacatata aggacttaat
     2881 ccttttgtaa tcagttccga caataggcag ctttatattt agtttttcta ataaaatttc
     2941 ctttgatacg ctgttatttt tatccagaga aaataattct ctttgccaat tatagaaata
     3001 tagagtttgg ttcttatcgg taaaaaaatc aaagaaaaat cctttactca tcagggaatt
     3061 caggaaaggg gagtttttgc cgttatagat tttattttta taaatatagt taacagaacc
     3121 attataattg aagaaccaaa gacgacccct tgggtcttcc ttgatgcgga cgacatcgtt
     3181 actgctcagc ccgtcttttt ttcggtaact cttaaaattg gtgccgtcaa atttgattac
     3241 cccggcctcc gaggtaaacc ataaaaattt gtggctgtcc tgatatatct gataaatggt
     3301 gtttgtaggc aagccgtcga gggtggtgta atgccgtata aaaggatttt gggcataggt
     3361 attggcgcaa aaaccaagga ggatgaaaat gaaaggatta agtaccttct tcaaattaat
     3421 cgattgaaaa tgaataattt ttaaccctgt cacgaaattc cgataatttc cgtctcgaaa
     3481 tatcaagccg ggagccatcc attaattccg catagttgcc ctgaacacag gaataaccgg
     3541 tcagaaaatt caaattgatg atggttgact tatgaatcct gaaaaattcc gggctttcca
     3601 gaattttctc aaactctccg agcgtcctgg ttgagatgat ttccctttta tcggtcaaag
     3661 agaaaaccgt ataattatcg gctgcctgca gatacatcac ttcgtcaaca tttaccaacc
     3721 ggaatccgga ttgttccgga attgtcagtt ttgtaacagg attgctcctg gagctcagat
     3781 tttctgtcag gttgttcaat gattgctgat atacttcctg gaattcggaa tatccattac
     3841 gaagttcatg gtaatagatt gctttcttca ccgcggcctg aagctcaata ggattaatcg
     3901 gttttaaaag atagtcaatg gcattggctt tcagggcgcg caacgcatgc tcctggtatg
     3961 ctgtggcgaa tattattccg tacttgtggc tggggattga ccggagaaat gtaaatccgt
     4021 cctcttttgg cattgcaatg tccagaaaaa tgaaatcgac catgttcgtt tcaagaagca
     4081 ttctcccttc gcctgccgaa caggcgatac cgcaaacttg aatttcaggg cagtattgtt
     4141 tcaataatat cgtcaatgct tcacgaatat tcttttcatc atcaataata atggcattat
     4201 attttttcat gcgccgggat attatttctg ttctagaatc ttaactaatt catattgaaa
     4261 taattaagat tttcaattca aaaaatgaac aagggttgaa aatagcaaaa tttcttaaaa
     4321 gaggcttccg gttttaataa acaaagaggc ttttggatga aaaattcggg tcagtggtga
     4381 gatttacgcc gagttttctg aaaccggctt catctccgtt tgcaggcaga tgtgtgagat
     4441 gaacctcaca acccctgagt tccttcaact tatcgacagc catttgggca gccgggtttg
     4501 agatggcgct gatgcccaga gtagttaatg tttcgtccac atcaaggctt atctttttgc
     4561 gccgcagtac ttcctttttc aggtacgcaa ttgatgcgat gacattttcg gggattaatt
     4621 gtagctggtc gggcaggcct gctagctgtt tcgctgcatt tagtatgaga ctcgatccgg
     4681 catgcatcag gggggaattc ttcccggtga tgatagttcc gtctttcagt tcgatggccg
     4741 ctccgcaata cattccatcg ttgcctttac cgtttaattc agcatcgcgg gcagctttcc
     4801 tggccggttc caccacatga cggtcttcaa cttttgcatc cacgcgtttc atgatcattt
     4861 ccgaccggtg taatgtgtct tcgtccatca ggccagttgc ataatcacat gcgcatttca
     4921 ggtaccggcg gatgatttcc tgatgagcgg cctgttgaac aaatccatgg tccaggattc
     4981 caaatccagc cttgtttacg cccatatcgg taggcgattt ataaaatgaa ggttctcccg
     5041 taatcttctc gatgattttt cgaagaagcg gaaaagcttc aatatcacgg ttgtagttaa
     5101 cggtagtttc attataagcc tcaaagtgat gcggatcgat caggtttttg tcattcaggt
     5161 ctaccgtagc tgcttcgtag gcaacattca ctttatggct tagcggaaga ttccagatcg
     5221 ggaaagtttc aaatttggca tatccggctt taacgcctct cttgtattcg tggtataact
     5281 gacacaggca ggttgccagt tttccgcttc ccggccccgg agccgtcact atcacgatgg
     5341 gcctggaggt tttaatgtat ttattggctc catagccttc atcgctgacg attctgtcaa
     5401 tatccgcagg gtaccctgca gtcggattgt gtgtggttac ttttatgccc ctccgttcga
     5461 gcttgttctt atataccgtg gctgcatact gcccggtata gcgcgtgatg acaacgtttt
     5521 taacttcgat gccccaatct ataaaatcat caatggtttt aagcgtatcc gtttcatacg
     5581 ttattccgaa atcagcacgg atttttttac gttcaatatc accggcataa atgcacatga
     5641 taacttccag tttatcctta agcttttgca gcagcctcat ctttacatta ggatcaaaac
     5701 cgggcaagac ccttgctgca tggtaatcgt acatgatctt accgccaaac tccaggtata
     5761 acttatcgtt aaagcgttta accctattta gtatctcgtt cgattgttct tcgatgtatt
     5821 tttcgttgtc gaaccctatt tgtgaattca tgaaggaagg aatataaatg gttaacctgc
     5881 aaggttaagg ttatttccga taaaggagta tgtattgtta atatttattg aagaattgcg
     5941 gcgcaggaaa cactggccgg cccttatgaa aaccaggatt ctggttcatc tcccgcaaat
     6001 tccggatctg ttgtgccggt attggctaca gatatcccgg agatgaacag catacttttc
     6061 ctgatagctt tcggaaactg tcttaaattt gaggcctgtt ttttgatcac ggaaaggaat
     6121 gtcggtcatt ggtcttattc ctaaagatat cagggcgata aagggcgttt tttcgtcgct
     6181 tcgctcgcgt aactaccgga tttattttat cgggcagggc gtatcgctga tcggcacctg
     6241 gatgcagaat atcgcactga gctggctggt ttaccggctc acaggatccg tgtttttgtt
     6301 gggccttatc ggttttacca gccagattcc gacatttatt ctatcgccat tcacaggggt
     6361 ggtaaccgac aggcacaacc ggctcaacat catgaagctg acccagcttt tcttcatgct
     6421 tcatgcactg actatgaccc ttctggtgat gttcaacctg gtacaggtat ggcatattat
     6481 aaccctgagc gtcatttttg gcattataac ggcatttgat gcgccagccc ggcaatcgct
     6541 tgtcatcgac ctgattgacg atcccgggaa cctgggtaac gccatcgccc ttaattcggc
     6601 cattttcaac gccgcacggc tggttggccc tgccattgca ggaattgtca ttgccattgc
     6661 cggcgaaggc atctgtttcc tgttcaatac ccttagtttt gccgcggtca tatttgccct
     6721 catgcaaata aagatttctt tcaggaaggt tgttggtgag ccggtcaatt ttaaagaaag
     6781 tttcagggaa gggtttgatt atactttccg cacaatgccc atcagaacgc tgattattat
     6841 gctggcctta ctgagcgttg tggggctttc atacatcgtt ctgcttccgg cttacgccaa
     6901 ggaaaacatg catggaagtt ccgacacact gggatatctg atgtcggcaa tgggagccgg
     6961 ggcattatcg ggagcccttt acatggctgc ccgcaaatca gttctggggc tggccaaagt
     7021 gatttctaca agtgtgatgg tgatgggcgc cgcgatttca ttggcttctt tttcgggaaa
     7081 catgttttta tcggtggtgg cattgttttt cggcggctta accatggttc tttccctgtc
     7141 ttcgatcaat acaatgttgc aaaccctagc cgacgaggat aagcggggac gcgtgatgag
     7201 cttctatgcc atggctttaa tgggcactac cccgataggc aaccttcttg ccggatcgat
     7261 tgccagtggg attggtattt catggacatt actgatgagc ggggtaatta ccgtaatttc
     7321 aggaatctgg ttcaggatga agcgtaaatc gctgcggaag catgtcaggc ccatttatgt
     7381 cacaaaaggg attttacccg gtctgcccaa tgatcttacc tgaagggcct gaaaattttc
     7441 agattctgct tttgagcgct ataagtcctg aacacctgcc agcctggcac ttagcaggaa
     7501 caaaaaggtt caccggtaca tgctcctgtt atcactgcct gaaggccgga tgccggtgtt
     7561 cagttccggg cgctttactt ttattcctga tgcttcaggt tcagagccct gccggcagga
     7621 aaaccctgaa ttcacttccc tccccgggtt cgctttttac ctcaatccgc cccccgtgct
     7681 tttcaacaaa ttccttgcat aatatcaggc caaggccagt gccgggttcc tggttggtgc
     7741 ctttggtctt gtgagaggta tcaatgctga acagggttcc gagcttttcc cgggcaatgc
     7801 caataccatt gtccctgacg ctaaggatac agaaaccttt attcatcgat agaccgacat
     7861 gcactgcgcc attttgcgga gtgaatttca gcgcgttggt aagcaggttc cttaaaaccg
     7921 tattgatcat gttcacatcc actctggcat tgcagcaatc tttgaaatcg gtatggattt
     7981 cgatgttctt acggacagct tgtacaccta ccagttcaat gctcttttcc agcaaggttt
     8041 tcaactcaac cggttcaggg tcaaaggcca tcgttccggt ttgtgaacgt gcccaaagca
     8101 gaaggttttc caggagttca tgagcctgct gtgaggaacc tttaatattc tcaagggtct
     8161 tttgtatttt tcgtgtatcc tggtatggaa tatccagcaa caggatgtcg gtaaggccga
     8221 taattgcatt aaagggattc ctgagatcat gggcaatgat tgaaaaaaat ttgtcctttg
     8281 acgcattcaa ttcattgagt tcaatgtttt tattaatgat ttccagtttc tgttcttcaa
     8341 cctggagtgt ccgttccctg acttttgctt caagtaattt ccggtccctg acatgatgtc
     8401 tctcccttag cttgacaatg aaaaacactg ataaaatgac aagaagaaag taaccaatat
     8461 aggctgccgt gctcctccac cagggcggac ggatgataat tgtgagattg cttccggctt
     8521 cattccacaa gccgtcgttg ttgcatcctt ttactctaag cacatactca ccaggcggga
     8581 gatttgagaa agcgacaaaa ttccgggtgc cgatatcgat ccagtcatca tcaatgcttt
     8641 ccagtcggta cttatacctg tttttggcgg agttcgtgaa ttccagcgcc gagaattcga
     8701 tggtaaacga gtaatcgtta tggttcagga cgatcttgtt acttttttca aaatcaagaa
     8761 attcgtttag tcctttcttt gctttgtaag cagatgtaaa taccactgac ggaagatagg
     8821 ggttgttcaa caaagaatca ggatagaagg agttgaagcc attcatgcct ccgaaaaaca
     8881 cttctccgtc tttgctgaga taagaagctc tcaggttaaa ctccgggctt tgtaagcctt
     8941 cctcaacggt atagacccga aaaccgttac cggtagaatc gaaacggcac aatccgtttc
     9001 cggtggcaaa ccagaggcac ttgtttttat cttccaggat gctgtaaacc aggttgcttg
     9061 gtaatccttg ctcttttgaa tagaattcaa agatgttgct ttttttcgag tatttattca
     9121 cataggaact ggttccaatc cagatgtctg cgttatggtc ttcacataaa accgttatga
     9181 aattatcagc aagcgagttt ttcccttccg ttttcgtgta atgcttcatc tgagaagtct
     9241 tcggattaaa gacatcaagc ccgtcaatgg ttccgatcca gatcagtcct tcccggtctt
     9301 ccagaacgga gtacaccagg ttggcgctta tacggtgttc aggtaacgcc ccgacatgaa
     9361 accgttccaa ttccgtcccg gcatgttttt tcctgtaaag gccatcctgc gttgctatcc
     9421 agaaatcgcc atcatggcct tgcatcatca tgaaaattct tagcccgcgg aggtcaggca
     9481 aaccgggatt tcctgagaac cggccgggcc tcacgaaacg atgtgattct ttatgaaaaa
     9541 tcagcagacc atcgcgggtg cctagccata catgctgacc tgaatcttcg aagatcgtat
     9601 gaacaaaatc gttggaaatg aaattcctgc cttttaacct tgatgaaaag tgctcaacct
     9661 tcccggtttt cctgttataa atgtttaatc cctgtcccca ggttccgatc cagatgttat
     9721 cgctgtcgtc tttgtacaaa gatgcaatta cattgcctga aagatcgacg gaatatgggg
     9781 agtcactttt acggtacagc ctgaatttct gttttttaag atcggtttta ttgatgcctg
     9841 acaatgttcc tacccaaagg ttctccgacc ggtcgatagc cagggagagg atgatattat
     9901 ggctgaggct gtttttttca gccgaatatg cttcaaaccc gagctttcca ttttttccgt
     9961 taatcatgcg tgtaaggccg cctccttcgg ttgcaatcca aagggagccg gtatgatctt
    10021 cgataatagc cctgataaaa tcattgctga cggaatttgg gtttttagcg tctttgaaat
    10081 agcgatcaaa aagttttcct gttttgttaa accggttaag ccctttcgaa gtgccgatcc
    10141 agatattgcc cttccggtcc tggaagagtg cggttatatt atcatcggaa atagtggacg
    10201 gatcggataa attatttctg aaaaccttga atttggagga acctgggtca aaacgggcaa
    10261 gtccacgggc agaacccagc catatcaggc catcggcatc ttcgagcaaa gggatattgt
    10321 aatctttcac accgccgtca taatcaaggg gagcggcgaa atggcggaat gtcatcgttg
    10381 ccgggtcaca gattgttaaa acgggcggag tgttgatgag tattttgccg ttgcgggcgc
    10441 atttaacacc gtatacatat ttgctgatat ccgggaaacg ggaaaaggtg tagcgaatcc
    10501 tatcgaagcg tttttcattt ctcacgtatt tattaagccc tcctttggtg ccgatccaga
    10561 ggtttccatc ccggtcctcg tctatgctat aaatccagtt gttggagatt gatgttgaat
    10621 cgtcaggatt ataggtgtag atgtcgaaag tatagccgtt gaatttgttc agaccatttt
    10681 gggtgccaaa ccagagatat cctttggagt cctgaagaat gcatgttacc acagattgag
    10741 aaaggccatc ggcgatagag tatcctgaaa agaaatattg ctgggcattg caacccaggc
    10801 caaaaaggaa aagtatcacc aaagaaagga ttttctgttt catctctttg aatgcagcaa
    10861 tagagccggc atttaagcaa tgccttgaat caaacggcag aatagcggac gggatcagaa
    10921 cagaagagag gaacgagctg tatgagatga tcaaaaatag caaatattat ttcaaattgt
    10981 catattggca acccgaattt ccggttaccg gtgaccagcc atgtttcaac ttctcctgag
    11041 atggaattgc agaccaataa taataccgga aagcatgaaa gcttttctcc cgcggttgct
    11101 ttcccattct tcagaagccg gcaaaatatt ttaccgggag gtggttagcg gcgcttcacg
    11161 gccaaaaaat ctgctttcag aaatttactt tgtttcttct tttggcggaa ggtaataacc
    11221 ttcacgcggc agcaggtggc tgaggtcagc gacgccgagt accacgatgg ccgtgagaat
    11281 ggaagtgtga ttaagatatt ctggaattgc cttgttgaaa aggtcccttt cggtatgcca
    11341 gatctccccg taattgaaat catagccttt cgggtcttcg caaccgaaag taatgacagg
    11401 gatgcctctc acggcaaaag gcccggaatc ggtgccccag gctttcttcg gcaattccat
    11461 gggttccatt tttttcagtg tgaaaggaaa ttcaggattg atggagttta aaggtttgca
    11521 gatattctca aaatcttcca tcatggcttc cggaacgctc aggcttttgg caacggtggg
    11581 tccgccgtcg cggttaaaca tgtttgaaat cttattccat ttgtcttcat tgcgtttcac
    11641 ccagctttcc gatcccagga gaccaaactc ttcaccggcc cacaggcaca ccaggattgt
    11701 gcgtttgggc tttgcccctg atttcatgat cagcctggct gcttccatag ccggtgcaat
    11761 tccggaacca tcgtcaaccg tgccggttgc cacatcgtaa gaatcgaggt ggccgcccat
    11821 gattacatat tcgtccgggt actttgtgcc ggggatgacg ccgatcacat tgcagtactt
    11881 tatcggcccc attttaaaat agttgcggat gtcgaactcc agttcaaatg attgacgctc
    11941 ttcagtcatt tgctcaatca ctttgtattg atgttcatcc agctttatat cgggtattgc
    12001 cggcaggttt tcaaatgtca tgctgtcgat gttattccgg tcatataatg cgcggatcgg
    12061 cacaggcgct gattgtataa ttccgaggat gccggcttcg gccatttgcc tgtaaaacaa
    12121 ggccggttct tccttatatg gcaataagtc aattttcgga ttgtcatggt ttttccagtt
    12181 caaacccctg attgaatcat tcttcttttc aatcagggca ttttcggcaa tgactttagc
    12241 ccgtttcgca tcggctgctt ttgatatatc gaccgggaag ccgttgcttt cccccgaaat
    12301 aagtacccag gcgcctttca gtttcccctt catcctgtcg aactcggcct gtgtttttgg
    12361 ttccaggaca acatgccctg tctgtacacc tttggttccc gctgtatagg acggcgtagc
    12421 gaaatgcaac gtcattccgt cgcttccgag catccttccg gaccaggggc cccggttgaa
    12481 gccaaccgga agggtgccgg cttcatccat ctcaaccttc attccccatt ccctgaattt
    12541 cgatgcagcc cattcagcag cgttttcata tgcatcggaa cctatcggcc tgccgccgat
    12601 acggttacaa agcacattga ggtggtccat tgtctggtta tcggttttac cggtttcaat
    12661 gattttgtcg gttatccggt catgctgtgc ctgtaaactg gttgtaaaaa acagtggaat
    12721 ggcaagtact gcgagaagtg agagatgttt cattgtttat atgctttgac taccgcaata
    12781 gatatttgcg aatgctgcaa aataacgaaa agaaagaaag tgaatcttta tacgaacttc
    12841 gtttttaagg aaacggttta ccgccggggg gatcaattga aaagccattt caaatggtat
    12901 gatcttacgc gtcttttttg gcaagcgcta attcccattt ccaggccgac gccatcatct
    12961 catccaggtt tttttcggct ttccagccaa gttcttcatt ggcgaaacgg gtatctgccc
    13021 acacctgttc aatatctccg gcacggcgcc cgacgatttt atagtttaat ttcaagccgc
    13081 ttacccgctc aaaagcctca atgatctgca tcaccgaaaa tccgtttccc gtgcccaggt
    13141 tgaaaacttc gggatttgac ctggaccggc cttccatcat acgctttagg gctattacat
    13201 gcgccttggc gaggtcgaca acatgaatat aatcgcggat ggctgttccg tcgggagtgg
    13261 gataatcatt gccgaaaacc cggagataat ccctttttcc gatcgcagtc tgggtaatat
    13321 acgggatcag gttattaggg atccctaacg gcaattcacc gatcagggcc gaaggatgtg
    13381 cccctatcgg gttaaagtag cgcaatgcaa tggcatgtag cggatgtgcg ttcacggtaa
    13441 acttcaggat atcctcacac atctgcttgg tgttgccgta agccgaaaat gcaggtttca
    13501 tcggggcgct ctccgttacc ggcagctcat ccggctgacc gtaaacggtg cacgaagagc
    13561 tgaagacgat attgccaatc ctgtgcgttt tcattccttc aagcaggttg atgagcgaca
    13621 gcaggttgtt gcggtaataa tccaaaggct tttccattga ttcgcccacg gctttatagg
    13681 ctgcgaaatg tataatcccc gaaatatcag gatgccgtga gaagaaatca gcagtcagtg
    13741 aagaatctgc caggtcaaat tgttcaaatt caggttttgt tcccgtaatt gcggcaatat
    13801 tgtccagtac ttcaatcatg gaattcgaca ggtcgtcgac aattattacc tgaaaaccac
    13861 tctgctgaag ttcgactacc gtatgtgaac cgatgtatcc tacaccgccg gtaacaagga
    13921 tttttcccat aataatgttt tcagaatttt taaggatgat caggaaccga acgttaaaca
    13981 gcacatcaaa ggcttatatg tcctgagaat attcctgctg atcaaaaaca aaagtacaaa
    14041 taacccttca tcgccggttt aataaaagcg gagttgttat acagcctgcg gaattttcgg
    14101 atttagaaat ttttatcttt aacacttttc aactatcaat atctttgtca ctttaaaaat
    14161 caaatatagc actatgaaaa tagccgtagt tggtagcggg tatgtaggcc tggttaccgg
    14221 gacatgcttc tccgaagttg gtattgatgt ggtttgtgtt gacattgacc agaaaaaggt
    14281 tgataacctg aagaatggca ttgtccccat atatgaaccc ggtctggaag agatgcttca
    14341 ccgtaacatg aaaaaaggcc ggttaaattt tacgacaagt gtggccgaag cgcttatcgg
    14401 cagcgaggtg gtttttattg cggtgggcac tcctcccgac gaagacggca gcgccgattt
    14461 gcgatatgtg ctggctgtag cccgtgagtg cggtcaacga atgaacgatt acctgcttgt
    14521 ggttaccaaa agcacggttc cggtgggaac ggcctctaaa gtgagggctg ctttacagga
    14581 agaactggac aaacggggcg tatccattcc tttcgatgtg gcttccaacc ctgaattcct
    14641 caaggaaggt gcggctattg aagatttcct gaaacctgac cgtattgtcg tcgggctgga
    14701 ttctccgcgg gctgaagaac tcatgaaaag cctgtacaaa ccctttaccc tgaacggcca
    14761 cccggtgatt ttcatggata ttacctctgc cgaaatgacc aaatatgcgg ctaactcaat
    14821 gctggccacc aagatcagct ttatgaacga tatcgcaaac ctgtgcgaaa ttgttggggc
    14881 cgatattaat atggtgcgca agggaatcgg ttccgactcg cgcatcggta caaagttcat
    14941 ctatcccggc atcgggtatg gcggttcctg ttttccaaaa gacgtgaaag cgcttatcag
    15001 cacagccggc gattataact atgaactgcg tgtactgaaa gccgttgaag cggttaacaa
    15061 cgaccagaaa tcggtgttgt tcaacaaaat catgaaattc tttaacggtg acattaaagg
    15121 caaaaccgtt gccatgtggg gtttgtcttt caaacctcag accgacgaca tgcgggaggc
    15181 gccgtcgctg gtcatcatca ggaaattact ggaagccggc gccatcgtaa aggcctatga
    15241 cccggttgcc atcgcggagg caaaacatca tctgggcgac agcattattt atgctgatga
    15301 acagtacgac gccctggttg atgccgattg cctggccctg gtcactgaat ggcccgagtt
    15361 taaatttcct aacttcagga ttatcaggaa actgttaaat tttccggttg ttttcgatgg
    15421 ccgtaatatt tatgacaagg atgaaatgaa acggaacggg tttgagtatt tctgtatcgg
    15481 agttaacact gaaaacagcc aggaataaat agttgacgtc tttttgtttt ccatcaacca
    15541 accgggtaaa aaagtcaatg atcagaaaaa aagtattggt taccggcggc gccgggtttg
    15601 tcgggtcgca tttatgtgaa aggctcctga acgacggcaa cgaagtggta tgcctcgaca
    15661 attattttac gggacaaaaa caaaatgtga tccacctgct gaacaatcct tattttgagt
    15721 tgatccgcca cgatgtaacc atgcctttct tcatcgaagt ggatgaaata tataacctgg
    15781 cctgtccggc ttcgcccatt cattaccagt ataacgccat caagacgata aagacttcgg
    15841 tgatgggcgc tatcaatatg ctgggccttg cgaaacgtat cagggcgaaa gttttgcagg
    15901 cttcaaccag tgaagtatat ggcgatcccc aggttcatcc tcaggtggag tcatactggg
    15961 gccatgtgaa tccaatcggg gagaggtcct gttatgatga aggcaaacgt tcggctgaaa
    16021 ccctttttgt gagttatcac aaacagaaca atgtcaaaat caagattatc agaattttta
    16081 acacctatgg cccgcgaatg catcccaacg acgggcgcgt agtttcaaat ttcattgtac
    16141 aggcgctgca gaacaaggat atcaccatat atggcgacgg gaagcaaaca cgtagctttc
    16201 agtatgttga cgatctgatc gagggtatgg tacgaatgat ggcaacacgg gacgagttta
    16261 caggtccggt caatatcggc aacccgaatg aatttacaat ccttgaactg gctgagaagg
    16321 tcattaaact taccggctca aaatcaaaga ttgtacgtat gccacttcct cccgatgatc
    16381 ccacacaacg tcagcccgat atctcgctgg caaaaaagga acttcactgg gaacccaaaa
    16441 ttcaactgga cgaagggctg acgaaaacga ttgattattt caggtcgata attgtataaa
    16501 tttcccgtaa tcagccatca tagtagcaga aaggatttta ttttacgata tttgttatct
    16561 gttccaagga tcaggttttc gttattttat tgccataagc caggagcacg gtaaaccgat
    16621 taatactctc agccatgaaa catgctgatt ttatttaccc gaccgacagt acaaagggcc
    16681 ttaaggactt taatccttcc ttttcaggga agtataagag caaaaagagc gcggaaaagg
    16741 acctcgaaaa agggattgag caactgtcgg tgatgcagga catgctttat gcccatgacc
    16801 ggtatgcggt attgatcatt ttccaggcga tggatgcagc gggcaaggac ggaacagtaa
    16861 aacatgtaat gtcggggatc aatccccagg gttgtcaggt caccagtttc aaggttccgt
    16921 cagccgagga acttgaccac gactacctgt ggcgttgcgt gaaacagttg cccgagcggg
    16981 gacggatcgg catcttcaac cgttcctatt acgaagaagt gctggtgacg cgggtacacc
    17041 cgcaaattct gcagaacgag aagctgcccg gcctgcccaa agcgcctgaa aatgaccgga
    17101 aattctggaa acaacgtttc gatgacatca ataatttcga gaaatttctc acgaaccagg
    17161 gtaccattat cctgaaattc tttcttcacg tttcgcctga agagcagaaa aaaaggctgc
    17221 ttgaacgtat cgacgacccg tcgaaaaact ggaaattctc taccggcgat gtaaacgaac
    17281 gcgccctttg gccgaagtat atggaagctt atgaagaaat cctgaagcat acttcgacgg
    17341 aggttgcccc ctggtatgtg attccggcgg ataaaaagtg gtttatgcgg gcagccgtct
    17401 gcgatgtaat cgttgaaagg atgcagcagc tcaaactcca ttaccctttg ctgaacgaca
    17461 ctcaaaaggc cgacctgtta aaagccaaag aaatgcttga aaacgagaat actgaaaaat
    17521 aatgctgtgt aaatctcgtt tgtaagacta tatttttcag gatgatcatt cgtgctagat
    17581 catcatcctt cgttaaacga tatcaataac tcaatgggcc ttaaaagcag caaatattac
    17641 aaccagaaaa aacgctggaa attctttctc ctttttgcag cagtcatcat cgtagtggca
    17701 tctttatggt tctcaaacct gttggtgcac aaaatcgcac aggatgaacg ggcaaaaatc
    17761 caaacctggg ctaatgccat caaccaccgc ctggctcttg tcaattcgac cagcagattt
    17821 tttgaacaga tacgcgacga agaacgcaag cgtgttgaat tatacgctgc agcccagaaa
    17881 agggctgccg aagcggcaaa tgatgatgct ttcctttctt tttattatca gataattgaa
    17941 aataacacat caatacccgt aatactcacc gatgaagatg gaaatataaa taccaccaga
    18001 aatatcgagc tggacaccga gaagattaaa aagattactc ctgagattaa aaagaagtta
    18061 tcgttatacc cgccggttgt cattgattat tatcagggga acctgaatta tctgtattac
    18121 tccgattcaa agctgtttac cgaagtccag aaagttcttg acgagatcat caattccttt
    18181 ttccgcgagg tggttgataa ttcggcagcc gtgccggttg tgatcaccga cagcacctgc
    18241 cgtcatgtta tcgcttcggg caacctggat gccgatcgcc ttactgattc cgtttacctg
    18301 aaacaactga ttgctgagat ggtttccgaa aacgacccga tcgtgattac ggttgctgat
    18361 cgcggtaaaa actacatttt ttacaaggat tcgtacaccc ttacccaatt acgatacctg
    18421 ccctacatcc tgttttttat catcagcatt ttccttttcg ttgcttacct cttgttcagc
    18481 acagcccggc gttcggagca aaaccaggta tgggttgggc tcgccaagga aacggctcac
    18541 caactgggca ccccgttatc gagcatgatg gcgtggattg actatctcga aacgaaaggg
    18601 attgattcgg atactatcgg cgaacttcag aaagacgtca acaggcttca aaccgttacg
    18661 gaacggttct caaaaatcgg ttcaacgccg gtgctgaaac ccgaaaatgt tgtggaactg
    18721 gtttataacg ccgtggcata tctcaagacc cgtacttcac aaaaggtgca gttttcgatc
    18781 aatgtaacgg acgataaagt aatcatggcg ccgatcaact accatctctt cgactgggtg
    18841 attgaaaacc ttgtgaaaaa tgccgttgat gccatgtcgg ggaaagggaa gatcaaaatt
    18901 gacctttacg aggatgatct ttttgtgaaa attgacgtcc aggataccgg gaaaggtgtt
    18961 gcccggaaaa tgttcaagac tatttttaac ccaggatttt caagcaagca acggggatgg
    19021 gggctgggac tttccctctc caagcgaatc atcagcgaat accataaagg taaaattttt
    19081 gtgaaggaat caacccttgg aaaaggaacg actttcagga tcgtattaaa aaagtagaaa
    19141 tgttgaatgg ataatggttc agggaaggga aacttcacaa cgggtttccc gacggactga
    19201 taaccttttc ctgttaattt cagattcatg gccggtatct atatccatat tccgtattgc
    19261 aggcaaaagt gcagctactg caatttttac tcgctggcca ccacgaagta tcgggagagg
    19321 ctgattcctg ccctggttaa tgaaatgcaa ctgcagcgcg attaccttga aggggaaatt
    19381 gtgcaaacga tatatttcgg cggcggaact ccttcgttga tgcgtgccgg tgaaatcaac
    19441 agggtgcttt cggcagtatg taatattttc ggggtcgaca gccaggctga aataaccctg
    19501 gaagccaatc ccgacgacct cagggctccg aaactccacg cactgaagca agccgggata
    19561 aacaggttaa gtatcgggat ccagtcattt gaagatgccg acctcaggtt cctgggacgc
    19621 agtcactccg ccgcacaagc ccgtaattgc attgacaggg cacaaaatgc cggattcagc
    19681 aacctgagca ttgatctgat ctatggtatt cctacgctcg acagtgaaag ctgggaaagt
    19741 aacctgttaa cagccctgag ttgcggcata ccgcatatat cggcctatgc ccttacggtg
    19801 gaggaaaaaa caccgatggc tttgaagata aaacgcggga gtattcccga tgtggacgac
    19861 gaggtgcagc ttcagcattt cggtatcctg cggtcattgc ttcaggctca tggttatcag
    19921 cactacgaaa tctccaactt ttgcgtaccg ggaatgtttt caaaacataa tacatcctac
    19981 tggcagggaa aaaaatacct tgggataggc ccttcggccc attctttcaa ttgggtgtcg
    20041 aggcaatgga atattgccgg tatcgggccg taccttgaat ctgtgaataa gggcattgtc
    20101 ccctttgaga tggaaatgct gacgaaagct cagcgtttta acgaatatgt gatgacatcg
    20161 ctgaggacca tgtggggctg cgacctgaaa gagattgaaa aaatgttcgg agagagttgg
    20221 gcctttcagg cgcttgctga tgcacgccaa tttctcgaaa acaagatgat ggtaaggaaa
    20281 gatggtgttc tttatcttac aaatgacggg cttttccacg ccgacggcat tgcatcggag
    20341 ttcttcaggg tagagtaggg gatagctgtt tttattttaa ggagttgatg taacaggaga
    20401 ttccgtatca ggagtccggc gttatcaggt atttgtctgc tccggcattc catttctttc
    20461 cggagccgga atatgtgaag atccgtcact gatttgtaga agccgtggct acgggctgtt
    20521 cagccgaaga atcgtcatct tagcccaacc gccattccta ccggctggtg atcggaaaac
    20581 cggaagcccg tttcgatggt ctttacacct ttaagttcaa tgttgggcga cagaacaaaa
    20641 aaatcaataa gggtaacggg cgttacccct gtgcggtaag gtgttcttac atcgcggttt
    20701 gtgggcagga aagaatcgta agcccactgc cagttttcgg gaagagcatg ttcgggaatg
    20761 ccgggatgaa tgcaccttgc atcgtacccc ttggagacgg cagaggaatc atagggcagg
    20821 ggattctggt tccagtcgcc gccggcaatc acataattac ctttgtcaaa ttccgctttc
    20881 atcagtttct tcagcatgcc gagttcctgt atcctcatgt cggcagcatc attgaaagcc
    20941 gaattatgca ggtttatgag caccaattgt ctgccgttcg aaacgttgta tcgggaaaga
    21001 ataaaacacc ggtcgagcat aaaaaggttt ttaggccagc tatagcacga cggataggcg
    21061 tgacgttcag cggaaactgg ccggaaacgg ctcaaggtca gttggccggc aataaccttt
    21121 cccatagggc ggaaaacagg cgccgggacg aattttacat tatagttgac ggcaaatgca
    21181 gccgaatatg acgggaaacc tgcttcgatc cgctgtatct ggttatcgcg gtatgatcgt
    21241 tttgcgaggc tgtctacctc ctgtaaaagg ataaaatcag gcttgttgaa taatgtggtc
    21301 atctggtaaa taacaccgtc acggtatctt tgatactctt ccctggatgg acggaccctg
    21361 cgtccgcctt cataaaagaa atccatttgt tctcccagcc cgcagtagcc gatattccat
    21421 gtgtaaaatg taagacttga atctgcaggc agtggtataa cgaatgaatc acctttcact
    21481 ttaacaggtt cgatagattt aggtcgaaaa tctgacacgg agataaagac aagcaggagc
    21541 gcaactgtta aaacagcagc aagcaacggc cataacagaa tatacaggat aaggtttttt
    21601 tttgttttca tagggcaggt tccgggcata agtcattaaa ggacaatatt tctcatctcc
    21661 acggttgcat aaatattcaa tgaaagcaga gcactcaacg aatttaaaaa aaatagttaa
    21721 tttcgctcag gttttgatta cccaaaaagg aacaaccatg ccaattctcg gttcaataat
    21781 taagagtgct cttgaggtgc gaaacaggat accggtcgaa aaactcaagc cgcaggatcc
    21841 atacaatgtt caggtgcgta ccttgcgaaa actgcttcgt acagcccagc tcactttttt
    21901 tggtgaagag tatggctttt cgcagatgct gcacagcgat gatgtggtgg atgtttttca
    21961 gcaaaaagtt ccgttgcatg attacaattc gatgcaccgg cgctggtggt accgcaccct
    22021 gaatggagaa gcctttgtat gctggccggg gcaggtcaaa cactttgctt tgagctcagg
    22081 cacttcggag gcttcgagca agtatattcc ggtaacgccc gatatgctcc gcgctatcaa
    22141 gaaaaccagt atcaagcaac tgctgtcgct taccaaatat gattttccac ccgagtttta
    22201 cgaaaaaggc gctcttatgc tgggtggcag cactcatctg cagtataacg gcacctatta
    22261 tgaaggggac ctttcgggca tcactacggg caacattccc ttttggttcc agcatttcta
    22321 taaacccggc aaccgcatat cgaaggaacg caactggtcg acgaaactcg aagaaattgt
    22381 aaaaaaagct ccggaatggg atataggcgc catcgtgggc gtccctgcat ggctgcaaat
    22441 attgctggaa aagatcatcg accagtacaa cctgaatact attcacgata tctggccgaa
    22501 cctgaacatt tatgtccatg gaggcgtttc gttttcccca tacgttaaag gcttcgaaaa
    22561 attgttggct cacccgctta cttatgtgga aacctacctg gccagcgaag gctttatcgc
    22621 ttaccagagc aggccggggt ccaactccat gcaactggtt cttgacaatg gaatattctt
    22681 tgaatttgta cctttcacgg atgagaattt tgaagaagac ggcacgctga agaccactgc
    22741 cgaagcgctt accatcgacg aagtggaaga aggccgcgaa tatgcattgc tgctttcgag
    22801 ttgcgccggg gcctggcgtt acctgatcgg cgataccatt aagtttacct cgaaagcctt
    22861 atcggaaatt gtcatcaccg gacgcaccaa gcattacctg agcctttgcg gcgaacacct
    22921 ttcacaggag aatatgaacc gcgctatcga gatgcttgaa catgaactga atatttcaat
    22981 ccgtgaattt accgttgccg gaatcaggca tggcagcatg tttgctcaca aatggtatct
    23041 tggcaccaac gataaagtgg atactgaggt tgcccgacgg aaaattgacg aatacctgaa
    23101 aatactgaac gacgattatc gtactgaaag gcttcacgcc gtacgtgatg taatggttga
    23161 aatattggat ccggaagtgt tttataattg gatgcgcctg cagggcaagg aaggcgggtc
    23221 tttcaaattt ccgcgtgttc tgaagaatgc caagtatgaa caatgggatg aatatctttc
    23281 ttcattaaag aaataaattt gacggccatt gcgttgattg ttcgtttaac aaccctatcc
    23341 ctccgaccgg catctgcctg aggataaatt gtagcttacc acgtgttaac cataaagcat
    23401 tttgaagtat gcatccatta atacaaggca tgatcctcgg tcttaccctg gcagccctgt
    23461 taggcccggc actcttcacc ttgcttcaga cgagcattca caggggctgg aggtcagggg
    23521 ttttcctggc tattggtatt atcgttagcg acttcagtgt actgatgctg gcgtatctcg
    23581 gggcgcttca actcatcaat aaaagaaata attacctcat tgccggcatt ataggcggca
    23641 tcatccttat ccttttcggc atttatacct tcaaccgtaa gatacatttt gatgaaaaca
    23701 acaatcccgt cgaaacaaag gttgcaggcc cgctgacgta tattctcaaa ggttattttc
    23761 tcaatattat gaaccctttt gtttggttct tctggatttc atccatggtg ggggtaagct
    23821 caaatttcgg ggatgataaa cacagcataa tgattttctt tatcgggact ttgtccatca
    23881 ccttttcaac agatattctt aaagtattcg cagccagtaa gttaaaacgc catttgaatg
    23941 gaaagatact gcttttcatc aaccattccg tcggaatcat tttaatgctt ttcggagtct
    24001 ttttaatgat caggacggtg gtgcaattct gaaccggcca ccggctgatc ctgtttcacg
    24061 aaaatgtgtt tatgctgaaa acggaagccg tcaggatccg gaaatgaaaa ggcaggttcc
    24121 ggccttgtgt cagtcatttt caaggtgttc tgaggcgctg tccaggttcg ggattgcgtg
    24181 gtcttggccg ggtttaaagt aaggaccctg aaatatggat atccggtatt tgataacaac
    24241 cgataacaac cgggagccat ccatcatttg agctccttca gccttcatcc ctgactgtta
    24301 taattctcct tatttccact ggtctttgct gcttttcagc ttatcgataa gccggcgctg
    24361 ttttttggtt ggccggccaa gcccgcggtc acgatattcc gaatttgttt cctggattaa
    24421 tttaacgcgg tcgtattctt cctgtggtgt gagatcttcg agataatccg gaaccagttt
    24481 ggcgctgagg cggcttttgg gaaaatcttt caccctcacg gttttgaaca atggattcag
    24541 gcttacaatg atgatttcac cggttttgat ctccttggcc ggtttcaccg aaatattttc
    24601 gatttttact tttccggctt tacaggcttc cgtggcctgg ctgcgggtct tgaaaaggcg
    24661 tacttcccaa agccacttgt cgatgcgggg atgttcgctt tccatgattc cggaaattat
    24721 tatttttttc tgaaatatac ttccatggag gctcccgtga attcaaacat ttcgcggagg
    24781 cggttctcgg caaagcgctt gtaaggatcg cggatgtatt gcggcagatt gcagaaaaac
    24841 acaaaactgg gataaaccgt tttaatctgc gtgatgtatt tgattttaac atccttgcct
    24901 ttgtacaacg ggggaggggt ttcagtaaaa acgggcagca gcgagtcgtt cagtttccgg
    24961 gtggcaatgc gccgtttacg gttttcgtaa acctttacag ccagttcaag ggctttatgg
    25021 attcgttgtt tcgtcagcac cgaggtgaat acaatgggat aatcggtaaa aggcgcagtt
    25081 tgttttcgta tatattcctc gaagtgcttc gttgagttgg tgtctttttc cacaagatcc
    25141 cacttgttaa ccaccataac aacgcctttg tgattttttt caataaggga gaaaatattc
    25201 atgtcctggg cttcaaatcc ctgcgtggca tcgatgagca ggaggcaaac ctcgctattc
    25261 tcaatggcgc ggatggaacg catcacggaa tagaattcaa tattttcata aaccttgcct
    25321 ttcttgcgta agccggccgt gtcgaccaac aggaaatcga acccgaatga attataacgg
    25381 gtaaaaatgc tgtcgcgcgt agtgccggga ataggggtta cgatatgacg gtcctttccc
    25441 agcaaggcat tcaccagcga tgatttcccg acgttcggtc ttcctaccac agcaaatttc
    25501 ggaaggtcag gtacttcttc ggtttccgtt ttccggaaag aagcaacaat ttcatcaagc
    25561 aggtcgccgg taccgctgcc attggccgat gaaacaggga agacgccatc aaggccgaga
    25621 ctgtaaaatt cagcggcttc atttacctta tccggggtat caattttatt aacagccaga
    25681 aaagctttct tgcgcgagcg cctgattaat tcagccacat cctcgtccag gggagaaagt
    25741 ccttcgcggg catcgaccat aaaaagaata atgtcggctt cctctatagc cagctcaacc
    25801 tgacgggtta tagcctgttc aaaaatgtca tccccgccga tgacgacgcc gccggtatca
    25861 atcaccgaga actcgatgcc attccagtcg ctgtgcccgt aatgccggtc acgtgttacg
    25921 ccgctggtcg gatcaacgat agcggcccgt gaaccggtaa ggcggttaaa taatgtgctt
    25981 ttgccaacgt taggccggcc aacaattgca aggatgttac ccatattgta aagtggaatt
    26041 tcggtacaaa ggtaaccctt tcttggttag gaacctgcaa gagggtatct ttcagcaggt
    26101 ttaccgggga ggagataacg agcgccggga ttccatatat ccagggtaat ttcataatag
    26161 ttgattatca cattgttgtg atcaggctca cttcccgctg attctgccgg tttcgcaccg
    26221 aaaggttttt atcaaaaaaa actgcatttt ttcaatttcc agcctataac caattccgtt
    26281 aaaagctata aataagaaac gaaaggttat gagaagcagc gagtatttac agaacgacaa
    26341 ttttgacgca gggatctgcc ggattgtcag caagcgcgga gtaactttta ccgtgtttaa
    26401 aaatgcgatc aggctcaata gtgaattgag ggaaaaaatt catgatgtca ttgaccttca
    26461 tctcagtgaa tatgctttcc ggaataacgc tgaaatcatt caggctgcca gggaaaaagc
    26521 cgaaaacgaa agcaccgagc taatgatgaa attcctgaga gagggcggaa attataaagc
    26581 aatagaagca aaactgcacc gaaacaggat tatcaaactg cttgcacaaa aagataaaaa
    26641 agaacaggcc gaactgataa aaaaatatac ctatgacacc cgtgcatcct tttgcggatg
    26701 catctaaaga aaaaggacaa ggcaacgatc ttcttactga ctcatcacac tctaaaatac
    26761 tgcttcagaa aactcccatt actcgttgac tcgtcttaac ccctgcatct taattgatgc
    26821 agggtttttt ttaaatggct ggtttgccgt tgaaatgtat tatgaaacag tatgtggctg
    26881 aacctgcatc attgagcgat gcgctaacga aagcagtaaa ttgttaaaac ctggccattg
    26941 tgaaacgatt tcatttatat ccggaaatat ttaccggaaa gttaagtata cactagcggc
    27001 caatgattct gggccggcct gccctttgcc ttccggacat ggccattgtt tatcgttccg
    27061 gacaagccgg tactcttaat aaattacatt gtctaaacct gcccgatgta gtttatcgtc
    27121 atatcaaaat gaaaatcgtc cgcctgcttt ttatcagggg aaccggcagt atgtttatgt
    27181 atccagttcc aggttctcac ggcccggatc atcctgttca acatacgggt aaaatatcct
    27241 atacccagaa tcagcatcat gatgaggata acagccattg caaacaatat cccgtcgttt
    27301 gcgttgccgg tccctgcaaa gctgctgctg ccagcaaaca gaattgccaa tagtataaaa
    27361 tagcgtttca tagctaaccg gttttagggg ttgaatatca atctgacttt cagtcaattt
    27421 tattacaaat gtaattcata tcagtggaga atgtttctta ttattgtaaa atgatcggag
    27481 gataaatgaa atgaaatcat ttgcggacaa aataatagat ttcaacggat cccttgattt
    27541 caagggtact ttaccggccg gcatcagcct gatgaaccct ttcagggaaa acagtgaaat
    27601 cttatcggta tcatcggcat tttaccgtaa atattacagc gacatcaacc ctcggcacct
    27661 gatcctaggt attaaccctg gcagatttgg cgccggagta accggcatcc cgtttaccga
    27721 taccaggcgc ctcgccgatg aatgcggcat caactatacc ggcaggcaga catatgaaac
    27781 atcgtcggta ttcgtctatg aggtcattaa agcctatggc ggaccggaaa agttctacgg
    27841 cgatttttac atcagcgcgg tttgccccct gggttttacc atacacgatg ccaaaggcag
    27901 tgaaaaaaac tataactatt acgacagcaa agaactgact gatgccgtat atccatttat
    27961 ggtggaaagc ctcaggatgc agatcggctt cggaataaaa acggatgtcg gtttttgcct
    28021 tgggaccggg aagaatgaaa aatttctgcg gtcgttaaat gacaagtata agtttttcgg
    28081 tgagataatc gccctggaac atccccggtt tgtgatgcaa tataaagccc ggtcgaagca
    28141 gcaatttatt gacaagtatc tgaatgcgtt cacaaatgtg aaatgatccg gttaatgatt
    28201 tcaggcaaca cggtgcagag aattatccat gagctgacca ggatcacaaa ctcaatgatt
    28261 attcttgtct tttaaaaaat tatcccggca ggataatata tttgtatata cctttacaac
    28321 ccaatgattt ggatgccata atacggagta ttttagtaac tatcatccca tgaagagaat
    28381 catacgcttc accttgcata aatatttatc attcagtagg ttaaatttac ttcttctcag
    28441 cctgttgatg ttcactttcg tgatttcatc acttaaaagt tacggagaag ggacaaaaca
    28501 actggaaccg gatgggccgg cttctatgac aaatgggggg cttggcctga ttctgtatga
    28561 tggtgggaat actacttatg gatacaggat acctttcgca atggccggat gtgattctct
    28621 ttacaggctt aatgtatata ttgcaaatcc ggcaactgaa aaaatttact ttgggttcag
    28681 aaaaggaagc tataaccagc tttattatca gtttcgtggt ccgaacgggg atgtggttcc
    28741 gggttatagc tttaatcctt caccggttgc cggtgagccc ggatttattg atgattggca
    28801 ggctgcagta aacgggccgc tgacaggtta cacgccgtta atactagatc cctctacggt
    28861 gggccttccg ggcaattact atatcgaatt tgccggcagc gcaaccggcg gcggaatcgg
    28921 taatgatggc gtgaccatta atcttattga tatcagtgta gttgcttcag gcggtactat
    28981 tattgatgga agggtatgga gttacgcctg gcagttttcc aaccagataa acggaacttc
    29041 tcacgcccaa aattccgatt tttattttta ttcaaacgac ggtctgacta caaaatgcaa
    29101 tatcaatagc tggggaggtg gtactttcta tgtatattgt aaccaatggg gtgtttacaa
    29161 taccgacaat tgggaaaatg acaggagatc agtagattat acctatggga ctgcgccttt
    29221 acctcttcct cagtacagag agttccttaa tcctccggac gtcaatgttt atcctaccgg
    29281 cacgattggg caaatctgca gtggaagctt gggaagcatt tcctattgcg acggatcggt
    29341 ggattttatc atgaaagtca caaaaacagg aatcgttacg ctgaatatta caacacccag
    29401 cggttctgca attgttcagg gagaggtaac cggatatgcg gattgttcca cctttgatac
    29461 gatccactgg gacggcatga tcaatggcac cccggttcag aatggggcca gccttagcat
    29521 gagtattcaa tatctgaacg gtttgacgaa cctgccatgt aatgatattg aaacctgcaa
    29581 cgacggtatc aaggttgatc tggtccggcc cgaaagcgct gctggtaaac tgggtatcat
    29641 gtgggatgat tcaaatattc cggttccgaa tgcaggggtt aattctgcct atcccggatg
    29701 tatatatccg agcagccccg ctatagacgg atgccattcg ttttccggcg ataagcggat
    29761 aatcaattcc tggtggtact acctgaccac ggggaacgaa tccctgaata tcgtcacccg
    29821 gaataccccg gccacgccga cggtatcgcc gacgggcccg tcgcctgtat gccagggcca
    29881 gacgggggcg a
//
LOCUS       DAOPYV010000037        29307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_1581_length_29307_cov_11.513435, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000037 DAOPYV010000000
VERSION     DAOPYV010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29307
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1581_length_29307_cov_11.513435"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..1492
                     /locus_tag="PKI35_06460"
     CDS             368..1492
                     /locus_tag="PKI35_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HNX44064.1"
                     /translation="MRYRKSLYLLPILAIILLATGKLLAQQNSVSRRFAVIAYHHGPG
                     EIAAAYPVEKLTHIIYSFLHLDGNCLAAGTTDSISIVQLVGLKQRNPNLKIILSLGGW
                     GGCPTCPDVFATETGRSEFSNSVKELMIKYNVDGIDLDWEYPALGSIPGYRHYPEDKQ
                     NFTMLIRSLRKALGDGYEVSFAAGGFAGYFIKSVEWTEVMPLVDRVNLMTYDYINGYS
                     TKTGHHTPLYSTSQQKESTDYAVRFLDSLGVPLEKIVIGAAFYARTFSHVSHRNNGLY
                     RNCKFEGFTGFRDLDEYFSRHDGFKVYWDSIAQAPYAYNPKERIFATFDDSRSVALKT
                     NYALDKGLNGIMFWSLTGDNPQNGLIGIIYNTLNQNNGLK"
     gene            1489..1923
                     /locus_tag="PKI35_06465"
                     /pseudo
     CDS             1489..1923
                     /locus_tag="PKI35_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            2047..2190
                     /locus_tag="PKI35_06470"
     CDS             2047..2190
                     /locus_tag="PKI35_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNX44065.1"
                     /translation="MACNYVYDPAVGDPDRGIPPGTPFEDIPDDWKCPVCGAAKDMFD
                     EIY"
     gene            2370..3083
                     /locus_tag="PKI35_06475"
     CDS             2370..3083
                     /locus_tag="PKI35_06475"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNX44066.1"
                     /translation="MTPTALFNDIRFYCTANASDEMVMKYSRYFREGYDAYGLSLDQL
                     EEKVLDILEHEETTFDLILKTSRLLVKSPKYEETSFAILLTKAFAKHFTPDIFNEIEK
                     WFENGITNWAHADVISGELLIIFLKKKLVSLQAFENWRTADNKFQRRAVPVTLIKLLK
                     TTEDFTPLFSFIEPLMMDTEREVQQGLGWFLREAWKLQPEAAEIFLTKWKDSAPRLIF
                     QYATEKMTAEQKKRFKKGS"
     gene            complement(3230..4213)
                     /locus_tag="PKI35_06480"
     CDS             complement(3230..4213)
                     /locus_tag="PKI35_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX44067.1"
                     /translation="MEKLIRNTRVLVTGGAGFIGSNLVEALLQQDNEVVCLDNFSTGK
                     RKNVEPFLQNNKFTLVEGDIRNLTDCNKAVEGVGVVLHQAALGSVPRSVKDPVTTNSV
                     NIDGFLNMLVASRDAGVGRFVYASSSSVYGDHPGLPKVEKETGNLLSPYAVTKKVNEL
                     YAGVFAGLYGMETIGLRYFNVFGKRQDPEGQYAAVIPRFIRSLIRHSRPEIYGDGLQS
                     RDFTYIDNVVHANQAAALAPSASVNTVYNIAFSERITLNELFGMLRDNLACFDSEIAG
                     IEPKYVSERPGDIRHSLASIDKATELIGYQPQFPVREGLKKAISWYWENLK"
     gene            complement(4266..5321)
                     /locus_tag="PKI35_06485"
     CDS             complement(4266..5321)
                     /locus_tag="PKI35_06485"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNX44068.1"
                     /translation="MKRNRKAVIITVFMAIIAALLIINHSNSTLRGKDTNFAVSDTSN
                     ITRIFLADKDNNTCNLHREANGTWMLNGKYPVMNETKDGMLKTLLNITVKMPVAKAAR
                     NTVIKLLAAKSVKIEIYQQVYRIDLFDKVKLFPHEKCTRTYYVGDVTMDNRGTFMLME
                     GSENPCIVYIPSFRGFVAARYSALEKDWRDRNVFALKLPDIKSVEVLYNENPAWSFQL
                     TNNNNRNFVLTSVTGAQLTNYDTLKVIQYLGSFRNISFEAFMDDNKAFRDSIAATVPA
                     ISISVTDVKGIKKTAKLWHKKSPYKTVDLFENPMEMDPDRMFAMVNEGKDLVLVQSYT
                     FDKILRKLPWFTTGSEK"
     gene            complement(5389..7170)
                     /gene="gldG"
                     /locus_tag="PKI35_06490"
     CDS             complement(5389..7170)
                     /gene="gldG"
                     /locus_tag="PKI35_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HNX44069.1"
                     /translation="MVKFFKPTGISSSRRNIRRNSISQLLLGLVFIVLLNIIGSYLFY
                     RFDLTSEKRYSLSPATKKMLKNLDDVVYFKVYLEGDFPAGFKRLRNETREMLGEFRAY
                     SDNIQFEFINPSASSSQSERENTYQRLVESGLNPTNLQVRQEGGMSEQIIFPGALVSY
                     KNKEFPLELLHSQLGTPPEEVLNNSVQSLEYTLANAISKLSSSDRPSVAFIEGHGELP
                     ELLTADAQNSLSEYYKVERVKINGMLSSLTRRVPKGKDSTDYIVKNKYKAIIIAKPDS
                     AFPEKDKFIIDQYVMRGGKILWLVDPVFASMDSLQNQDQTIGIGMNLNLDDLFFNYGC
                     RLNPDLIMDLNAMRIPMVTGQMGDQPKMEMLPWYYFPVVFATSTHPIVRNLNALKTEF
                     VSSIDTITAPNIRKTILLSTSRYSRLLNSPALITTEVMRQQPDMARYNNPYRTIALLL
                     EGNFPSLFLNRIPPELAGAPEIQFIDHSKPTAMIVVSDGDIVKNQLQSSPTGPSPLPL
                     GYDRYSRETFGNKDFIMNAMNYLCDDSGLLSVRSKELKLRLLDGEKINAEKVKWQLIN
                     VLLPVLIILAFGVIQHFVRRYKYTRPF"
     gene            complement(7164..7895)
                     /gene="gldF"
                     /locus_tag="PKI35_06495"
     CDS             complement(7164..7895)
                     /gene="gldF"
                     /locus_tag="PKI35_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HNX44070.1"
                     /translation="MYTLLRKEVSSFLNSLIGYAVIIVFLLINGLFLWIFPMQFNILE
                     YGFAGIDNLFLLAPWVFLFLIPAITMRSFAEEQRSGTMELLLTKPLTDMEIILAKYLA
                     GVVLVFFSVLPTLIYFISVYLLGQPVGNVDMGGTWGSYLGLLFLGAGFVAIGIFASSV
                     SGNQIVSFLIAVFLCGFVYIGFEFIYGLSLFGRIDLFIQQLGISAHYASMSRGVIDTR
                     DVIYFLSLIAVFLMLTRFNLERRKW"
     gene            complement(8417..9196)
                     /locus_tag="PKI35_06500"
     CDS             complement(8417..9196)
                     /locus_tag="PKI35_06500"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNX44071.1"
                     /translation="MPIITLTSDWGLTDHYVASVKGAILRRVPGVTIVDISHEIRHFD
                     IKHASFVLRNSFRQFPEGTIHIIGIDSIESKNHQHLVVYAEGYYFIGADNGIFSLILD
                     NPPQKIISLNILQDTGYYTFPSRDRFAKVAGHIAEGKSIDELGEPVSALVPKSLYQPV
                     KEGNNILGRVMYIDSYNNVFINITEKLFRESVQNRPFSISFRRNSYSINTLVKAYGDV
                     PEGEMCALFSSNGLLQIAINRGKAASLLGLTIDYPVNVVVK"
     gene            9698..10669
                     /locus_tag="PKI35_06505"
     CDS             9698..10669
                     /locus_tag="PKI35_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNX44072.1"
                     /translation="MTEKTISLESYSPLEVYGVNDRNINLLHNLFPKLRIIARSDLLK
                     IQGEDQEIEQFEQVFTSMLMYLDNFGKLDEEDVRRLASNGNATEESVNFREVNADVLV
                     HGRNGMMIRARTPNQQKMVESISRNDMIIAIGPAGTGKTYTAVALAVRALKNKEVRRI
                     ILTRPAVEAGENLGFLPGDLKDKLDPYLQPLYDALRDMIPTQKLVSYLEDGTIEIAPL
                     AFMRGRTLEGAFAILDEAQNATEGQLKMFLTRMGKSSKFVITGDITQIDLPRNQNSGL
                     VQAMRILNDVKGIDIIMLDDRDIVRHKLVTNIINAYEKEKDQKSDKK"
     gene            10776..11729
                     /locus_tag="PKI35_06510"
     CDS             10776..11729
                     /locus_tag="PKI35_06510"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNX44073.1"
                     /translation="MQNAISRTNFSFPGQKDLYIGKVRDVYNINDELLIMVTSDRISA
                     FDVVLPRAIPYKGQVLNQIAARFLDDTADIVPNWKIAVPDPMVTVGRRCEPFKVEMVI
                     RGYLSGHAWREYKDGKRSLCGIPMPDGMKENDRFPEPIITPTTKAAIGHDEDISKEEI
                     LNLGLVEKEDYEKLENYTRAIFRRGTEIAAKMGLILVDTKYEFGKANDRIYLIDEIHT
                     PDSSRYFYKEGYEERQSKGEPQKQLSKEFVREWLMENGFQGKEGQQVPAMTDEFVNSV
                     TERYIELFESITGQKFERADLTDVPSRVEGNINRFLQAYYR"
     gene            complement(12084..12959)
                     /locus_tag="PKI35_06515"
     CDS             complement(12084..12959)
                     /locus_tag="PKI35_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNX44074.1"
                     /translation="MILSDDSMEFQALISLLDDPDPVAYNSVSKRIFDLGPPAVHVLE
                     DVWEHSFDELVQSRIEQIVHHIQFEDIRKKLSEWSSREENDLLDGYCIITRFQYPDVD
                     EVKLRDQVEKIRRDVWLELNDRLTALEIVKVFNHIFFEVHEFSQKTESGKIQDSGCYF
                     LNNLLESRRGNSLSLGVLYVLLAEKLNLPVRGVDLPGHFVASYMRSPELPGEMPGILF
                     YINPSARGAVFSRSDVELYLKQAGIGPEEKFFIPCSSKQIITRLLDDLKMMYDKQGDS
                     EKASDLKALKKILQY"
     gene            complement(13059..13931)
                     /locus_tag="PKI35_06520"
     CDS             complement(13059..13931)
                     /locus_tag="PKI35_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNX44075.1"
                     /translation="MFRIPESELILNGDGSVYHLHLKPENLAGTVIVVGDPGRVPEIS
                     KHFDRINFKVQNREMVTHTGYIGRRQLSVLSTGMGTDNLDIVMNELDAVVNIDLERRE
                     PRQEHTTLNIIRLGTSGSLQADIAPGEAVASTHGIGLDGLLYFYADNGKVTDADMANA
                     FVKHTGWDKNLPGIYAVPASQELLSTLGKGLKQGITLTAPGFYGPQGREVRLKTAFPQ
                     LNHLVESFEYQGNRIVNFEMETSALYGLGRLLGHETLTICSIVANRVAKTYAPDYKKD
                     IEKLIQQVLSRLVA"
     gene            14207..15454
                     /locus_tag="PKI35_06525"
     CDS             14207..15454
                     /locus_tag="PKI35_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNX44076.1"
                     /translation="MTAEIITIGDELLIGQVVDTNSAWMAEKLNEFGILVRQITSISD
                     DREHILNTLREASQRAEVILITGGLGPTKDDITKTTLCEYFGSTMVFSETAYEDVRHL
                     FRSRGLPVTGLNRGQAMVPECCTVIPNHNGTAPGMWFEKNSVIYVSMPGVPFEMKALM
                     TGEILPRLESKSENAIMHRTLLTEGIGESFLADRIESWENQLPSHIKLAYLPQPGIVR
                     LRLSARGTDKKKLGDELQKAVDGLLPLAGEFIFGEGDDTLEEVVGKLLREKGCTLATA
                     ESCTGGYIAHLITSIAGSSDYFKGSVVAYANEIKEKLLGVKPRTLAQHGAVSSETVKE
                     MAEGARERFTVDYAIATSGVAGPSGGTPEKPVGTTWIAVASPLGTVTRKFILGEHRGR
                     NIQRAAISALSMLRKIMIEQSRK"
     gene            complement(15471..16235)
                     /locus_tag="PKI35_06530"
     CDS             complement(15471..16235)
                     /locus_tag="PKI35_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX44077.1"
                     /translation="MPLHISSLNSGSNGNCYYIGNERDAVLIDAGISCRETERRMKRL
                     GLSLEKVRAVFISHEHTDHTRGVEVLSRKHHLPVYISAPTYQNSYLKLDPALLKHFDA
                     LSPLKIGCLDICAFPKRHDASDPHSFTISHDGVTVGVMTDIGSPCEHVVQHFSRCHAV
                     FLEANYDERMLDEGRYPYYLKKRISSDVGHLSNRQALELFRHHRPAFMTHLILSHLSK
                     ENNRPELVYELFGTHANGIQIAVASRFEESGLFCVG"
     gene            complement(16455..17546)
                     /locus_tag="PKI35_06535"
     CDS             complement(16455..17546)
                     /locus_tag="PKI35_06535"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HNX44078.1"
                     /translation="MKKLNMGGLVIPVPIIQGGMGVGISLSGLASAVANEGGVGVISA
                     AGLGMIYRDRSDNFLNNCVIGLREEIRKARAKTKGVLGVNIMVALSNYDDMVRTSIEE
                     GIDMIISGAGLPLDLPKYLTPGCKTRLVPIVSSARAAKLICDKWLTNYSYLPDAVIVE
                     GPKAGGHLGFKPDQIFDENFQLERLIPEVADTINMISTREGKSIPVIAAGGIYYGSDI
                     AEIFSLGASGVQMGTRFVTTNECDASDAFKQAYLNAAEDDVRIIRSPVGMPGRAIRSP
                     FLEEVEAGKRQPKTCPVNCIRTCDIRTAPYCIMASLTSALRGNFRRGYAFCGSNVWRT
                     NEIISVKELMQTLKMEYQACMAEAELVTC"
     gene            complement(17842..18702)
                     /locus_tag="PKI35_06540"
     CDS             complement(17842..18702)
                     /locus_tag="PKI35_06540"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HNX44079.1"
                     /translation="MFENLNQPYPFNNNFKHNLRTISFVSMGFVLIVLYFQPFGINFL
                     ASARDGYFVLVMGLVSAATFFLSTMIFPGLFPKLFNPDRWTIRKELIWNTGMIVVLAV
                     GFSVSALLFRISGLKSMTLFRSGALALLPLVLFNLINYNRSLKEKLVHAIDSGRHWLA
                     EERKSPLQAITDRVRIESESGKEFFEGEVRNIVLIQSASNYIEIFYRDGGVVRKQLLR
                     QTLTAVELKLSAFPCIRKCHRRCLVNSGQVLKLTGISPNYTLEAEGLDFRIPVSRQNV
                     AEFRKLVSSK"
     gene            18999..21071
                     /locus_tag="PKI35_06545"
     CDS             18999..21071
                     /locus_tag="PKI35_06545"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX44080.1"
                     /translation="MKTKLLTAFTILLLLTTFGLQLSGQEALSEKDARMAWWREARFG
                     MFIHWGLYSIPAGEWKGDTGHAEWIRTTARIPLEEYDKFVQQFNPVKFNAEEWVKAAK
                     DAGMKYIVLTSKHHDGFCLWDSDQTDFDVMSTPFKRDILKELADACRKYDMRICWYHS
                     IMDWHHPDYLPRRDWEKDRSAEGADFDRYVQYLKNQIHEILTKYGDISILWFDGEWES
                     TWNHDRALDLYNYICSFNKNIIINNRIDVFREGMAGLSSNEKALGDYGTPEQEIPDTG
                     LPGVDWESCMTMNDHWGYNANDHNWKTAGQLIYNLVDIASKGGNYLLNVGPTSEGVFP
                     PESIERLKTIGKWMKINGASIYGTQAGPFKNPAWGKCTQKETEGGTRLFLHIFGNPQA
                     GKINLPGLMNETVKAFLLSDKSETPLTITRKNGSVEITLPQSGLDEICTVVVLDMKGK
                     PEIIDKPEFSFRYDAASDKVEISLVSASTKPDLNVYYSTDGTLPTDNMSVFTKPVMLK
                     LPVTLKAVSYIGKKRVGNVAEVSLPVSFGKTVTLDNLPSEKYSAGGPASLTDGIRGSK
                     KFADGRWLGFEGSDAVACIDLGKKTAIRSINLGYLVNTGSWIFEPVEITVETSVNGEK
                     YTTALTRKYNAALWKQADGIREFADVFPEVNARYVKVTVKNRGVCPATHPGNGGKAWI
                     FLDEISVE"
     gene            21191..21940
                     /locus_tag="PKI35_06550"
     CDS             21191..21940
                     /locus_tag="PKI35_06550"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44081.1"
                     /translation="MKNLLTKSLSVVFLAFLLASCDDSGKKYVGLEPEQIKEKNFNGE
                     KKIYTPEGKLRTIAEYKNGKVDGRVRKFFDDGKIYMDATYQNGLKNGKCTYYYTNGKP
                     YTVTDYVNGYKEGVEKTYYQEGGLMAVANFRKNIIQPGLQEYKKDGKPIPVNTTIIIS
                     EVNHVRLDGTFRVQMRLSDKSVTPKFYIMEQSAPDKRQKVNMSNGTGTLVLNFNASSF
                     VMKKLIIEAEYKTYRGNLMKIRKPYNLAIDL"
     gene            complement(21947..23521)
                     /locus_tag="PKI35_06555"
     CDS             complement(21947..23521)
                     /locus_tag="PKI35_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44082.1"
                     /translation="MNLRLVILSIPFLLVFMSGRIVAQEIVADSCSGLVFSTKLESTG
                     FESFCSKKPDYLLMSVAVDPESTESDLADIRQMIKNETDILKMKTEKIKKPVKALQII
                     FSHVQTSFLKHYDLDAGFSKMFKTGYYNCLTATILYSLILDELGYRNTPKVLPGHVYL
                     IAYGGETPYLFETTDPEHGFMQLNDAVLTSTLQSLRLFKFIQSDNIENQPVGNIFDRY
                     YLKLNTTNLRGLVGYQYINAMVQASMKQHSLEAYQALNKAIVLTPLDELLIMKEQLLR
                     ASIAEMSLKSAERARYVVEYYNHTKNQNKEDQVIGEFSLMINQCLFGSFPTPDSLHTI
                     YNALVDGIQDTLVQSKLKELYYVNYTSYLLMNEETDKAFRIVYDAYRQVANSMQVKVI
                     LSKLVEFYTSTSYTDKRNFEVLDSLASEYPGIRELDYYRARRCYLTISLASKAFDENQ
                     IATGEKYLTMFESDAEALSQQELVFCDPAEAYGDAASCYFRKGNMTKARRVINKGLVY
                     SPGNWMLTAKLKQIDK"
     gene            23755..23988
                     /gene="rpmB"
                     /locus_tag="PKI35_06560"
     CDS             23755..23988
                     /gene="rpmB"
                     /locus_tag="PKI35_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNX44083.1"
                     /translation="MSRVCEITGKKALVGNNVSHSNIRTKRKFYPNLQTKRFYIPEED
                     EWITLKVSAAAIRTINKKGISAVLKKVSEKGYL"
     gene            24216..24866
                     /locus_tag="PKI35_06565"
     CDS             24216..24866
                     /locus_tag="PKI35_06565"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX44084.1"
                     /translation="MNRKYAFIIALLMATACAQAQHRDDGLVQFSGVIVTADSLRPVP
                     FTHILVEKTNRGTTSDYFGFFSIVVHKKDIIHFSAVGFKKVEFRIPDTLSENRYSLIQ
                     VMTSDTIMLAETVIYPWPTKEQFRQAFIKLDVPDDDLEIMRKNLAYLELRDRRDKNYN
                     PDLYGMDAAMNYRNYVDNYTNKMYYAGQAMPNSLLDPLAWLKFIKAWKRGDFKKKY"
     gene            24958..27501
                     /locus_tag="PKI35_06570"
     CDS             24958..27501
                     /locus_tag="PKI35_06570"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX44085.1"
                     /translation="MDREHFHQNRCYFSPLLLILFLGFYLPGQSQTFAKIYGKVTDER
                     NNAIEMANIAVSGYPGGVTSGKDGAYELRVPAMKPLIIIYSFVGYESDTLRITLDANI
                     NFRANKVLKQTAMLLPYLVVEDKALRNTNLSRIDPHTAAMIPSASAGIESLVKTMPGV
                     VSNNELSSQYSVRGGNYDENLVYVNDIEIYRPFLMRSGQQEGLSFLNSDLVSSVLFSA
                     GGFDAKYGDKMSSVLDIRYRKPSEFAGSASASLLGATGHIEGVSKNNRFTYLAGVRYK
                     SNQYLLNSLETKGDYKPRYFDIQSLLTYNVSRKVEVSFLGYIARNDYTLIPQSRETNF
                     GTMYDALRLKVYFDGREADNYRSYLGATTVTYKPTEHLNIKWIVSGYRSQENESFDIL
                     GQYWIQLLENAPDSIEVVENKGVGSYLTHARNELNMDVFNIENRYTLIHDNSNTQWGV
                     KYQHEIIQDQIDEWMLVDSAGFSTPYSPGIPGMAGNLTDLRLYSCLKTSADITSNRYT
                     GFIQNTRDLDGDSTKLTLTTGLRFSYWDYNRQFLVSPRATISFKPNWQRDILFRFSTG
                     YYFQPPFYRELRNLEGQVNPEVRAQKSIHLVLGSDLNFQAWDRPFKFVTEVYYKILKD
                     LIPYEVENVRIRYLGYNDSKGYAAGIDMKVNGEFVKGVESWASLSIMRTEEDLYDDYY
                     YEYYNQSGEKIIAGITTDQTVTDSVKVEPGYIPRPTDQRVTFGLFFQDFLPMNPTYKM
                     NLGLYFGTGLTTGAPNTPLYKHVFRMPAYRRVDIGFAKQIIGREAGQSSRKFFGNIKN
                     MWVTAEILNLLQINNTISYMWVRDFDGNQYGVPNYLTSRQINIKLSVEF"
     gene            27664..28281
                     /locus_tag="PKI35_06575"
     CDS             27664..28281
                     /locus_tag="PKI35_06575"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNX44086.1"
                     /translation="MIYQFPELPYAYDALEPYIDRMTMEIHHTKHHKAYFDKFLAAVK
                     DTGFDGKPLEEIIRNAGKAPAAVRNNGGGFYNHSLFWEVLSPTGGGNPAGKLADSLRK
                     HFGTFDEFKARFSDAAINRFGSGWAWLSVKSDGSLCVCSSPNQDNPMMDISECNGIPI
                     LALDVWEHAYYLRYQNRRPEYVSAFWNIVNWQKVNENYEKVLLTL"
     gene            28459..29214
                     /locus_tag="PKI35_06580"
     CDS             28459..29214
                     /locus_tag="PKI35_06580"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNX44087.1"
                     /translation="MKTQITFLSLSFLLCISASAQYNYGNQAGMLTTGDLKIYAQDAA
                     GHQTGFRFPALPYTYNGLQPAIDSLTVAIHYNRHHRGYYENFVATIKGTPLETMPIGE
                     IFAEISRQPVAVRNNAGGYYNHFLYWENMQITTGNMPSGDLEAAIIRKFGSVGAFREQ
                     FGNAAKAIFGSGWAWLILNKDKEPEITTTSNQDNPLMDIAPVQGIPLLALDVWEHAYY
                     LSYQNRRADYVNAFWNHINWDEVERRFSEAMKK"
BASE COUNT         8149 a         7285 c         6701 g         7172 t
ORIGIN      
        1 agctacaaaa aggctcgtca acaaccctct ccttgaggag agggcagggt gaggtgtctt
       61 gtaaacgctt atacccgtca acaacaaaaa gcctcgtcaa caaccctctc cttgaggaga
      121 gggcagggtg aggtgtcttg taaacgctta tacctgtcag ctacaaaaag gctcgtcaac
      181 aaccctctcc ttgaggagag ggcagggtga ggtgtctgcc gcgaacgata caagggctga
      241 aactgattaa tctttattgg attttcctga ggaagttccc ttttcatcat caccttatcc
      301 gccttcggca gacggtcgtt tgttatttcc agaaactgcg tatctttaac tttttcagaa
      361 aaaaggcatg cgttaccgca aatcattgta tttgttaccg atactggcaa tcatccttct
      421 cgccacaggg aaactgcttg cccagcagaa ttcggtctca cgccggtttg ccgttattgc
      481 ctatcatcac gggcccggtg aaatcgccgc ggcatacccg gttgaaaagc ttacccatat
      541 catctacagt ttcctccacc tcgacggcaa ttgcctggca gccggtacaa ccgacagcat
      601 ttcaatcgta cagcttgtcg gcctgaaaca acgcaacccg aatttaaaga ttatcctttc
      661 gcttggcgga tggggcgggt gcccgacatg tccggatgta tttgctaccg aaaccggccg
      721 ttcggaattc tcgaattcgg taaaggaact gatgataaaa tacaacgtcg acggcatcga
      781 ccttgactgg gaataccccg ccctggggtc aatccccggc tacaggcact atcctgaaga
      841 caaacaaaac ttcactatgc tgatccggtc gttgcgaaaa gccctcggag atggttatga
      901 agtgagtttt gccgccgggg gattcgccgg ttatttcata aaatcggtgg agtggaccga
      961 ggtaatgccg ctggtagacc gcgtgaacct gatgacctat gattacatca acggctacag
     1021 caccaaaaca ggccatcata cgccccttta ttccacttct cagcaaaagg agtcaaccga
     1081 ttacgccgtc aggtttctcg attccctcgg cgttcccctt gaaaagatag tgatcggagc
     1141 ggctttctat gccaggactt tcagccatgt cagccataga aacaacggcc tttaccggaa
     1201 ttgtaaattc gagggattta ccggcttcag agacctggat gaatattttt cccggcatga
     1261 tggtttcaag gtatattggg atagtattgc tcaggcaccg tacgcataca atcccaagga
     1321 aaggattttt gccactttcg atgacagccg ctctgttgcc ctgaaaacaa attacgccct
     1381 tgataaagga ttgaacggca ttatgttctg gtcgctcacc ggcgacaatc ctcaaaacgg
     1441 cctgatcgga attatttaca ataccctcaa ccaaaataac ggcctgaaat gaacatcgaa
     1501 tcgtttttca gcctcactta tggcttgtat atcgtgagcg ccggatatgg cggaaggtcc
     1561 aacggctata tatcgaatac cgtattccag gtgaccgccg atccgccccg gatagcgata
     1621 agctgcagta aaaacaacct gacaaccgaa atgattgccg gcagcggcag gttctccgtt
     1681 tcggtgctca atcaggaagc cagccctgaa ctgatcggcc ttttcggcta taaaagcggc
     1741 agggatatca acaagttcga tcaaacggag cacatcatca gcggtgcagg aacacccatc
     1801 gtgataaaag acgccattgc ctggttcgac tgcaaaacct ggattcaaac agatgtcgga
     1861 acgcatattc tcttcatcgg cgatgtaacg gatgccgaaa tcatcgacac atccatcccg
     1921 cccctcacct atgcatggta cagggcagta aagaaaggcc tttcgccgaa aaacgccccg
     1981 acctatattg acccggccaa actcgaaaga aagggaaaca aagacaccgg caagaaatac
     2041 cgttgcattg cctgcaatta tgtttatgat ccggctgtcg gcgaccctga caggggcatt
     2101 cctccgggaa cgcctttcga agatatcccc gacgactgga aatgcccggt ttgcggggcg
     2161 gcaaaagata tgtttgatga aatttattaa atatgagtac agatccccgg tcagccgttg
     2221 gtcccggcat ctccccttcc tgatccgatc aggtaccctg gctaaaatca tctcccggta
     2281 ataatgccgt ctgcaaatcc ctgaaacctg aatacacttt gatacttttt ccaattcttc
     2341 cgtttttttt aaacaattaa accaagtcca tgacaccaac cgctcttttc aacgatatcc
     2401 gtttttactg tacagccaat gccagcgatg aaatggtaat gaaatattcc cgctatttcc
     2461 gggaaggata tgacgcatac gggctttcgc tcgatcagct tgaggagaaa gtgctcgaca
     2521 tcctggaaca tgaggaaacc acttttgacc tgatactgaa aaccagccgg ctgctggtaa
     2581 aaagcccgaa atatgaagaa accagttttg ccatcctgct aaccaaagcc tttgccaaac
     2641 attttacacc tgatattttc aatgaaatcg aaaaatggtt tgaaaacggc atcaccaact
     2701 gggcgcatgc tgatgttatc agcggggaac tgctcatcat cttcctgaag aaaaaactgg
     2761 tttcactgca ggcgtttgaa aactggcgta cggcagataa taaatttcag cgcagggcag
     2821 ttcctgtaac actgatcaaa ttgctgaaaa ccaccgaaga tttcacgcca ttgttctcct
     2881 ttatcgaacc actgatgatg gatactgaac gtgaggtgca gcagggactt ggctggtttt
     2941 tacgggaagc ctggaaactg cagccggaag ccgctgaaat atttctcacg aaatggaaag
     3001 actcggcgcc aaggcttatt tttcagtatg ccacagagaa aatgacggct gagcaaaaga
     3061 aaaggttcaa aaaaggctcc tgatcaatat aacgccggca ggcaaccgga cacaggcccg
     3121 ggcatcagaa atatgcttac cgtcaataac aaatggctgc tgaccatccg gcagatttca
     3181 aaaccaaaaa tgtttatcgg ctataaacca ccctccatcg gagatccgcc tatttcaggt
     3241 tttcccaata ccaggaaatg gctttcttca gaccttcccg aaccggaaat tgcggttgat
     3301 aacctatcag ttctgttgct ttatcaatgg atgccagcga atgcctgata tcgccgggcc
     3361 tctcgctcac atatttcggc tcaatgcccg caatttcgct gtcgaagcag gccaggttat
     3421 cgcgaagcat gccaaacaat tcattgagtg ttatcctctc gctgaaggca atgttgtaaa
     3481 cggtgttcac tgaggcagac ggagccaggg cggcagcctg attggcatgc actacgttat
     3541 caatataggt aaaatcgcgc gattgaagcc cgtcgccgta tatttccggc cgggagtgcc
     3601 gtatcagtga tctgataaac cttgggatta cagcagcata ttggccttcg ggatcctgcc
     3661 gtttaccgaa cacattgaaa tacctcaggc ctatcgtttc catcccgtaa aggccggcaa
     3721 aaacaccggc atataattca ttgacctttt ttgtgacggc atacggagac aggaggttcc
     3781 cggtttcctt ttccaccttc ggcaggcccg gatgatctcc gtaaaccgac gaactggaag
     3841 catatacaaa acgcccgacg ccggcatcgc gcgacgccac cagcatattc aggaagccgt
     3901 cgatattgac cgaattggtt gtaacagggt ctttcaccga gcggggaacc gatcccaggg
     3961 ctgcctgatg cagcacaacg cccactcctt caacagcttt gttacagtca gtcagattgc
     4021 ggatatcccc ctcaaccagg gtgaatttat tgttctgcaa aaaaggctca acattcttcc
     4081 gtttgcccgt agaaaaatta tcaaggcaga ccacttcatt atcctgctgc aacaatgcct
     4141 ctacaaggtt ggaaccaatg aaaccggcgc cgcctgtaac aagaacacgt gtattcctga
     4201 tcaatttttc catagctgat aatcatgaat gaaatgagtt tcaaattctg taaaccacgg
     4261 cccggctatt tttcgcttcc cgtggtaaac cagggcaatt tcctcaggat tttatcaaaa
     4321 gtatacgatt gcaccagaac caggtccttc ccctcgttta ccatcgcaaa catgcggtca
     4381 gggtccattt ccatggggtt ttcgaagaga tcgacggttt tgtagggaga ttttttatgc
     4441 cagagttttg cagttttttt tatgccttta acatctgtga ccgatataga gattgccgga
     4501 accgttgccg ctatcgaatc ccggaaggct ttattatcgt ccatgaaagc ttcgaagctg
     4561 atattgcgga aagatcccag gtactgaatt accttcagcg tatcatagtt tgtcaactgg
     4621 gccccggtaa cagatgtgag aacgaaattg cggttgttgt tattcgtcag ttggaacgac
     4681 catgcgggat tttcattgta cagcacctca accgatttga tatcgggaag ctttaaagca
     4741 aaaacattac ggtcgcgcca gtctttttcc agggcggaat aacgcgccgc tacaaatccc
     4801 cggaaactgg gaatatacac aatgcagggg ttttcactcc cttccatcag catgaaagtg
     4861 ccgcggttat ccatggtaac atcgccgaca taatatgtgc gggtgcattt ttcatgggga
     4921 aagagcttta ccttatcaaa aaggtcgata cgatatacct gctggtagat ctcaatttta
     4981 accgatttgg ctgccaacag tttgatgacg gtgttgcggg cggctttggc aaccggcatc
     5041 tttaccgtaa tgttgagcaa cgtcttcagc atcccgtcct tcgtttcatt catcacaggg
     5101 tatttcccgt ttagcatcca cgttccgtta gcttcacggt gaaggttaca ggtgttgttg
     5161 tccttatcag caaggaaaat cctggtgatg ttcgaggtgt cgcttacggc aaaatttgta
     5221 tccttaccgc gcaacgtaga attggagtgg ttgatgatga gcaacgcggc gataatcgcc
     5281 ataaaaacag tgatgataac ggccttgcgg tttcttttca tggatggata atgatagaga
     5341 atgctttttc tgaagaacta tttgttacga taccacgtat ccggaatttt aaaacggacg
     5401 ggtatatttg tacctgcgga caaaatgctg aataacgccg aaagccagga taatcagcac
     5461 aggcagaaga acattgatca gttgccattt tactttttca gcattgattt tttcgccatc
     5521 cagcagcctc agttttaatt ctttggaacg gacagagagt aatccggaat catcgcacag
     5581 ataattcatg gcattcatga taaaatcctt gttgccgaaa gtctcacggc tgtacctgtc
     5641 gtaacccaat ggtaacggcg acggtcccgt gggtgatgac tgcaattggt ttttaacaat
     5701 gtcgccgtcg ctcaccacga tcatagccgt aggtttgcta tgatctataa attgaatttc
     5761 aggggcgccg gcaagctccg ggggaatacg gttgagaaag agtgaaggaa aattcccttc
     5821 caggagcaag gcaatagtcc tgtatggatt attatacctg gccatatcgg gctgctgtct
     5881 catcacttcc gtggtgataa gagccggcga attcagcagt ctcgagtatc tcgaagtgga
     5941 caggagtatc gttttcctga tgtttggcgc tgtgatggta tcgatactgc tgacaaactc
     6001 tgttttcaag gcattcaggt tcctgacgat cggatgggta gaagttgcaa ataccaccgg
     6061 gaaataatac cagggcagca tctccatttt aggctgatcg cccatctgcc cggtaaccat
     6121 tgggatccgc atagcattca ggtccataat caggtcggga ttcagccggc atccgtagtt
     6181 gaagaataaa tcatcaagat tcaggttcat gccgattcca attgtctgat cctgattctg
     6241 aaggctgtcc atactggcga aaacaggatc tacaagccac aatatcttac cgccgcgcat
     6301 cacatattga tcaatgatga acttgtcctt ttccgggaag gcggaatcgg gctttgcgat
     6361 aataatggct ttgtatttgt ttttcacaat ataatccgtc gaatcttttc ctttcggcac
     6421 acggcgggtg aggcttgaaa gcatgccgtt gatttttacg cgctccactt tgtaatattc
     6481 gctgagtgaa ttttgcgcat cggcggtcag caattcaggc agttcgccat gcccttcgat
     6541 aaaggcaacc gatggcctgt cgctgctgct taatttgctg atagcgttgg ccagggtata
     6601 ttcaagtgat tgaactgaat tattcaacac ctcttcaggc ggggttccaa gctgggaatg
     6661 cagcaattca agcgggaatt ccttgttttt ataggacacc aatgcaccgg gaaaaattat
     6721 ctgttccgac atgccccctt cctggcggac ctgaaggtta gtcgggttca ggccggactc
     6781 aaccagccgc tggtaggtat tttcacgttc gctttggctg gaactggctg aaggattaat
     6841 gaattcgaac tggatattat cgctgtaagc cctgaattcg cctaacattt cacgtgtttc
     6901 gttacgcaac cgcttgaaac cggcagggaa gtctccttcg aggtacactt tgaaatacac
     6961 gacatcgtcc aggtttttga gcattttctt ggtagccggc gataacgaat agcgtttctc
     7021 gctggtaagg tcaaaacggt aaaagaggta ggatccaatg atgtttaaca gcacaatgaa
     7081 aaccaatccc agtaataact ggctgatgct gttgcgccgg atattcctgc gcgatgaact
     7141 tatgcccgtt ggcttaaaaa attttaccat tttctgcgtt cgaggttaaa acgggtcagc
     7201 ataaggaaaa ctgcgataag actgaggaag taaatcacat cgcgggtatc gatcactcca
     7261 cggctcatcg aggcataatg ggcgctgata cccaactgct gtataaacag gtcaatcctg
     7321 ccgaaaagcg ataatccgta tataaattca aaaccaatgt aaacgaatcc gcacagaaaa
     7381 acagcgatga ggaatgaaac gatctggttc ccggaaacgg aagatgcaaa aatgccgatg
     7441 gctacaaaac cggcgccaag aaacagcaag ccgaggtacg acccccatgt tccgcccata
     7501 tcgacattac ctacaggctg gcctaaaagg tacaccgaga taaaatagat cagcgtggga
     7561 agcaccgaga agaataccag cacaacccca gcgaggtact ttgccagaat tatctccatg
     7621 tcggtgagcg gtttggtaag cagcaattcc atggttccgc tgcgctgctc ttcagcgaat
     7681 gaccgcattg tgatggcggg aatcaggaac aggaacaccc agggcgccag caggaacagg
     7741 ttatcgatgc ctgcaaagcc atactcaagg atgttgaatt gcatcgggaa aatccagagg
     7801 aagagtccgt tgataagcag gaacacgatg attaccgcgt acccgataag tgaattaagg
     7861 aagctgctaa cctcttttcg cagtaatgta tacatgctgt taacgttgcg ttttgacgcg
     7921 caaaaataac tattttgtta aagcggtatg gcattcttta taagatgata cgccaaccta
     7981 ccggcagaag acacattaat cttcctgaat aacggacaac ctgccggctt cagcccgaat
     8041 caaactttca tcacggccat cctgatccgg tcgtgaacct gaaacagcac atcgcgtcaa
     8101 taacatggtt gcccggaaaa taacttttta gtttttgatt tgccattttt attgcgtccc
     8161 tgccaggatt tgatcaggcg ccgcttcctg ttgcggaagc aggctcatct atggtattaa
     8221 cccggcaccg gcaaacaaat ttaaaatccg gctgagcggc gtcaggaacg aaataatcgt
     8281 ttcaacaaac aacaaccatt ctgaacccga acaacaacaa tataaaatta tggggatggg
     8341 tcatccgatt tttgaaccaa atgcctttat attcccgacc cggcaacagc ctgattgtta
     8401 ccccggcgtc tgtatcttac ttcaccacca cgtttaccgg gtaatcaatc gtcaggccga
     8461 gcagcgaagc cgcctttccg cggttaatgg caatctgcag caatccgttt gacgaaaaca
     8521 gggcgcacat ttcgccttcc ggcacatcgc cgtatgcttt taccagggtg ttgattgagt
     8581 atgaatttct gcggaaactg atgctgaaag gacggttttg taccgattcg cggaatagtt
     8641 tttcggtgat attgataaaa acattgttgt acgaatcaat atacattacc cggcccagaa
     8701 tattattacc ctcctttacc ggttggtaca acgatttggg caccagtgcg ctgaccggtt
     8761 ctcccagttc atcgatgctt tttccttcgg cgatatgacc ggcgaccttt gcaaaacggt
     8821 cacgcgaagg gaaggtataa tagccggtat cctgcaaaat attgagcgaa atgatcttct
     8881 gcggcgggtt atcaaggatc agggaaaaga tgccgttgtc ggcgccgata aaatagtaac
     8941 cttcggcata aacaaccagg tgctgatgat tcttcgattc gatggaatcg atcccgatga
     9001 tatgaatggt gccttcggga aactgcctga aggagttgcg gagcacaaac gatgcatgtt
     9061 taatatcgaa atgccgtatt tcatgcgata tatccacaat agtaacacca ggcacacgcc
     9121 ggagtatggc cccttttaca gacgccacat aatgatcggt aagcccccag tcgctggtta
     9181 atgtaatgat tggcatgaaa ctggataccc tgttcggaat agctgtattt ttgcatatca
     9241 aaagtagaaa ttattatgga gcccaaaacc ataaaggatc aaaactcagc atagaattag
     9301 ttaattcctt aaaaatctaa tttttaaaaa cactcaaacc ctgcaacgcc gaaaccctgc
     9361 cctccttacc ctcaacctcg cccagtcagg caagcctgaa accctgcaac tctgaaaccc
     9421 tgaaactttg aaactctgta accctgaaac tctgaaaccc tgccccctac cacagccttg
     9481 cgctaggcag gcaggtctga aaccctgaaa ccctgaaacc ctgccccctc ccacagcctt
     9541 gcgctaggca ggcaggtctg aaaccctgaa actctgaaac cctgccccct accacagcct
     9601 tgcgctaggc aggcaggcct gccacctcac acacagcctt cccaggcagg caggtctgaa
     9661 acactgcaac tctgaaactc ttaaacgccc atcccctgtg accgaaaaaa ccatcagcct
     9721 tgaatcctac agccctctgg aagtatacgg cgttaacgac cgtaacatca acctgttaca
     9781 taacctcttc ccgaaattgc ggattatcgc ccgaagcgat cttttgaaaa ttcagggcga
     9841 agaccaggaa attgaacagt ttgaacaggt tttcacctcc atgctgatgt acctcgacaa
     9901 cttcggcaaa ctggacgaag aggatgtccg ccgcctggcg tcgaacggaa atgcaaccga
     9961 agaatcggtc aacttcaggg aagtgaatgc cgatgtcctc gtccacggac gaaacggtat
    10021 gatgatccgg gcacgcaccc cgaaccagca aaaaatggtt gaaagcatct cccggaatga
    10081 tatgatcatc gccatagggc ctgccggaac cggaaaaacc tataccgccg ttgcgcttgc
    10141 cgtcagggcg ctgaaaaaca aggaagtcag gcgcatcata cttacccgtc cggccgtgga
    10201 agccggcgaa aacctgggat tccttcccgg cgacctcaag gataagctcg acccctacct
    10261 tcagccgttg tacgatgccc tgcgggatat gatccccacc caaaaactcg tttcttacct
    10321 cgaggacggg accatcgaaa ttgcaccgct ggcctttatg cgcgggcgca ccctcgaagg
    10381 cgccttcgcc atcctcgacg aagcccagaa cgccaccgaa gggcagttaa aaatgttcct
    10441 cacccgcatg ggcaaatcct caaaattcgt gatcaccggc gacatcaccc aaattgacct
    10501 gccccgcaac caaaactccg ggctggtcca ggcaatgcgc attctcaacg atgttaaggg
    10561 aattgatatc atcatgctcg acgaccgcga catcgtcagg cataagctgg tgacgaatat
    10621 catcaatgcc tatgaaaaag aaaaagatca aaagagcgat aagaaataaa cgacttgccc
    10681 tatttttgag gcgttaaatt ttttctctgc cggacaagca cagcgtcgtg aatttcaaag
    10741 cagtaacttc aaatcctaaa ccataattta ccgttatgca aaacgccatc agccgcacga
    10801 acttcagttt cccgggccaa aaagaccttt atatcggaaa agtccgcgat gtgtataaca
    10861 tcaatgatga actgctgatc atggtcacca gcgaccgtat ctctgctttc gacgtggtgc
    10921 tgccacgcgc catcccttac aaagggcagg tgctgaacca gatagccgcc cgattcctcg
    10981 acgataccgc cgacatcgtt cccaactgga agattgccgt tcctgaccct atggtaacag
    11041 tgggaaggcg ctgcgagccc ttcaaggtgg aaatggtcat ccgcggttat ctgtccgggc
    11101 atgcctggcg tgaatataaa gacgggaaac gctccctatg cggcattccg atgcccgacg
    11161 gcatgaaaga aaatgaccgg ttccctgaac ctatcatcac ccctaccaca aaagctgcca
    11221 ttgggcacga cgaggacatc tcgaaagagg aaatcctgaa cctcggactg gtggaaaagg
    11281 aagactacga aaaactggaa aattacaccc gggcgatttt ccggcggggc acggagatcg
    11341 ctgcaaagat ggggcttatc ctggttgata caaaatatga attcgggaag gcaaacgacc
    11401 ggatttatct catcgatgaa atccatactc ccgattcatc ccgatatttc tacaaggaag
    11461 gctatgaaga acgccagtcc aaaggcgaac cacaaaagca gctttcgaag gaattcgtac
    11521 gcgaatggct catggaaaac ggtttccagg gcaaggaagg ccagcaggtt ccggcgatga
    11581 ccgacgaatt tgtaaactcc gtcacggaga gatatatcga actctttgag agtatcacag
    11641 gccagaaatt cgaaagggcc gacctgaccg atgttccgtc acgggttgag ggaaatatca
    11701 acaggttcct acaggcatat tacaggtaaa agtttaccag gcgggagcag agaacggaga
    11761 acggaaaacg gagaacttta cctgccagtg tatggccttg tgtgagaggc aggcaggtct
    11821 gtctgcctgg cgcgagccta agggtggcag gcaggggaga atgaaaaact caaaacacag
    11881 aactcaaaac tcaaaaattt aagagccaaa agtttacctg cctaggcaag ggtgtgggca
    11941 agacggcagg ctaatggcta aaagccaaca gctaagagcc aacggcctgc ctgcctgggc
    12001 tggctgtgcg caagatggca ggtctacctg cctgggcgag gttgttggta atacggcagg
    12061 ccaaaaggca aaagtttgcc ttattaatat tgtaaaatct tcttcagcgc tttcagatcg
    12121 gatgcttttt cgctatcgcc ctgtttatca tacatcatct tcaggtcgtc gagcaaccgt
    12181 gtaatgatct gcttgcttga gcagggaatg aaaaattttt cctccggacc aatgcccgct
    12241 tgtttgaggt acaattccac atcactgcgc gaaaacacag ccccccgggc agacgggttg
    12301 atataaaaca gtattcccgg catttccccg ggcaattcag gactcctcat ataggaagca
    12361 acgaaatggc ccggcagatc aacgccacga accgggaggt taagtttctc agccagcagg
    12421 acatataaaa cgcccaaaga taatgagtta ccgcggcggc tttcgagcag gttattcagg
    12481 aaataacagc ccgaatcctg tatttttcct gattccgttt tttgactaaa ctcatgaact
    12541 tcgaaaaaaa tatgattgaa aaccttaacg atttccaatg ccgtgagacg gtcgttgagt
    12601 tcaagccaga cgtcgcgcct gatcttttcc acctggtcac gtagttttac ttcatcaaca
    12661 tcgggatact gaaaccgggt aatgatacaa tacccgtcta gcagatcatt ttcttcgcgt
    12721 gatgaccatt ctgaaagctt tttacggata tcttcaaact ggatatggtg aactatctgt
    12781 tcaatgcggc tttgaaccag ttcgtcgaag gagtgttccc aaacatcttc aagcacatga
    12841 accgcaggcg gacctaagtc gaagatcctt ttactgacac tgttgtaagc aaccgggtca
    12901 ggatcgtcga gcaggctgat cagcgcctga aattccatac tatcgtcgga caggatcata
    12961 ttacaaatat aatcccggtt tcaccgtgta aagggtatcg tttgcctgat ttttcaacaa
    13021 gggaacgttg aaagtcaagg gcccgaaaat gaacaggatc aggcaactaa acgactcagt
    13081 acctgctgaa taagtttttc gatgtctttt ttataatcgg gagcataggt tttggccacc
    13141 cggttggcaa cgattgaaca aatggtaagc gtttcgtgcc ctaacaacct tcccagccca
    13201 tataaggccg aagtctccat ctcgaaattc acaatgcggt ttccctggta ttcaaaagat
    13261 tcgaccaggt gattcagttg ggggaatgca gttttgagcc gcacttcccg gccctgggga
    13321 ccataaaagc cgggcgccgt gagcgtaata ccctgcttta atcctttgcc caacgtagaa
    13381 agcaattctt gtgatgccgg gacggcataa atacccggaa ggtttttatc ccatcctgta
    13441 tgtttcacaa atgcatttgc catgtcggca tccgtcactt taccattgtc ggcgtaaaaa
    13501 taaagcaatc cgtcaaggcc tatgccatga gtggaagcga ccgcttcgcc aggggcaata
    13561 tcagcctgca gagagcctga agtacccagc ctgattatat tcagtgttgt atgttcctgt
    13621 cttggttcgc gcctttcaag gtcaatattt acgacagcat ccagctcatt catcacaata
    13681 tcgagattgt cggtccccat gcctgtagaa agcacgctca actgccggcg gccgatataa
    13741 ccggtatggg taaccatttc ccggttttga actttaaaat tgatgcggtc gaaatgcttg
    13801 gaaatttcag ggacccttcc gggatcgcca acgacaatta cagtaccggc aaggttttca
    13861 ggcttcaggt gaagatgata cacactgcca tcgccattaa gaatgagttc tgattcagga
    13921 atacgaaaca tatctgtaac tttgtctcaa aagtacgaaa gaaccgccat ctgcggaaaa
    13981 aataacaaca gcgttgcgca aagccgcagt cagcatgcag caaactgcgg tcagtgcaat
    14041 aagcatgaag gagagttgcc gggaaccggt attgaccgtg atgagagcaa attttacctc
    14101 tcgctgtaaa acacgattca taataatacg cctcaccatc aatcctcccg cccgcatccg
    14161 ctgatggctg aaaccttacg caccattacc taaaccctga tactcaatga ctgccgaaat
    14221 cattaccatt ggcgacgagc tgctcatcgg gcaggttgtt gacaccaaca gcgcctggat
    14281 ggccgaaaaa ctgaacgaat ttggcatcct tgtccggcag atcaccagca tctccgacga
    14341 tcgcgaacat attctcaata cactgcgcga agcatcgcaa cgcgctgagg tcattctcat
    14401 taccggcggg ctgggtccca ctaaagacga tatcaccaaa acgacgctat gtgaatattt
    14461 cggttcaacc atggttttca gcgaaacggc ttacgaagac gtccgccacc ttttcaggtc
    14521 gcgcgggctt cctgttaccg gtcttaaccg cggtcaggcc atggtccccg aatgttgcac
    14581 ggtgataccg aatcacaatg gcacagcccc ggggatgtgg tttgaaaaga atagcgtgat
    14641 ttatgtttcc atgccgggcg ttcctttcga aatgaaagcc cttatgaccg gagaaatcct
    14701 tccccggctc gaaagcaaaa gtgaaaacgc tatcatgcac cgcacccttc tcaccgaagg
    14761 catcggtgaa tcatttctgg ctgatcgcat cgaatcatgg gaaaaccaac tcccttctca
    14821 tataaaactg gcttaccttc ctcaacccgg catcgttcgt ctgcgcttgt cagcccgcgg
    14881 cacagataag aaaaaactcg gcgacgaact tcagaaagcc gtggacggtc ttttgcccct
    14941 tgccggagaa ttcattttcg gcgaaggtga tgacaccctg gaagaagtag tgggtaaatt
    15001 gcttcgcgaa aaaggctgta cgctggcgac ggccgaaagc tgcacaggcg ggtacatagc
    15061 tcaccttatc acaagcatag ccggcagcag cgattatttc aaaggctcgg tagtggctta
    15121 tgccaatgaa ataaaggaaa aattgctggg tgtgaagccc cgaacgctgg cacaacacgg
    15181 cgccgtaagc agtgaaactg taaaggaaat ggccgaagga gccagggaac gcttcaccgt
    15241 ggattatgcc attgccacca gcggagtagc cggcccgtcc ggcggaacgc ctgaaaaacc
    15301 agtaggaacg acatggatag ccgtggcttc gcctcttgga accgttaccc ggaaattcat
    15361 cctgggcgaa caccgcggca ggaatatcca gcgggctgca atatcggccc tcagcatgct
    15421 caggaaaata atgattgaac aatcacggaa atagagtccg tcaggaaccg tcatccaaca
    15481 caaaacaagc ccgactcctc aaatcgcgac gcaaccgcaa tctggattcc gtttgcatgt
    15541 gtgccgaaca attcatacac cagttcaggc ctgttgtttt ccttcgacag atgcgacaat
    15601 atcaggtgcg tcataaacgc gggccggtga tgccggaaca gttccagggc ctgacggttc
    15661 gacaggtggc cgacatcgct gctgatacgc tttttcaggt agtaggggta tcttccctca
    15721 tcaagcatcc tttcgtcata attggcttcg agaaatacgg catggcaccg gctgaaatgt
    15781 tgtaccacat gctcacacgg gctgcctatg tcggtcataa ccccgaccgt aacgccatca
    15841 tgacttatgg tgaagctatg cggatcggat gcatcgtgac gcttcgggaa agcgcatata
    15901 tcaaggcaac ctatctttaa gggcgacaga gcgtcgaaat gcttcaacaa tgccggatcg
    15961 agtttcaggt agctgttctg gtatgtagga gccgaaatat aaaccgggag atgatgtttg
    16021 cgcgaaagca cttccacgcc tcgcgtatga tcggtgtgct cgtgcgaaat aaaaacagcc
    16081 ctcacttttt ccagggacaa tccgagccgt ttcatccgcc gctcggtttc acggcacgat
    16141 atcccggcat caataagcac ggcatcacgc tcattgccga tataatagca gttgccgtta
    16201 ctgcccgaat taagagagga aatgtggaga ggcatgatcc tgcaaagaaa attaaaaatt
    16261 ctctcaggaa ataggatgtt gaaaactagc tgaagataaa ttaccaggag gccggcagta
    16321 ttctcttctg aatttgtccg gcagattaat atatttcccg gaccgggctg aaatttctca
    16381 atgtaaaaag ccgtacagga gttcatccta tccggcttta ctttccatcc gacacttata
    16441 aattatatgg aattctaaca ggttaccagt tcagcttccg ccatgcaggc ctggtattcc
    16501 atttttagtg tttgcatcag ttccttaaca gaaataattt cgtttgtccg ccatacattg
    16561 cttccgcaga aggcgtaacc cctgcgaaag ttcccgcgca acgccgaagt gagcgaagcc
    16621 atgatgcaat aaggagcagt gcgaatatcg caggtgcgga tgcaattcac ggggcaggtc
    16681 ttcggctggc gttttccggc ctccacttct tccaggaaag gcgaacggat ggcccttccc
    16741 ggcattccca ccgggctcct gataatgcgg acatcatctt ccgctgcatt aaggtaagct
    16801 tgtttgaatg catcggaggc atcacactcg ttcgttgtaa cgaagcgcgt ccccatctgt
    16861 acacccgaag cccccaggct gaaaatttct gcaatgtctg atccataata aatcccgccg
    16921 gccgctatca cgggaataga ctttccttca cgagtggaaa tcatatttat agtatcggca
    16981 acttcgggaa taagtctttc aagttgaaaa ttttcatcaa aaatctgatc cggtttaaag
    17041 ccaagatgcc ctccggcctt cgggccttca acgatgaccg catcgggcag atagctgtaa
    17101 ttagtgagcc atttgtcgca gatcagtttt gcagcccttg cagatgaaac aatcggcacc
    17161 aggcgtgttt tgcaaccggg agtaaggtat tttggtagat caagcggcag ccctgccccc
    17221 gaaataatca tgtcaatccc ttcctcgatg gaagtgcgca ccatatcgtc ataattgctg
    17281 agcgccacca taatgtttac gcccagaacg ccttttgttt ttgcgcgggc tttcctgatc
    17341 tcctccctca atccgatcac gcaattattg aggaagttat ccgatctgtc gcggtatatc
    17401 attcccaacc ccgcagccga aatcacgcca actccgcctt cgtttgccac tgcagaggca
    17461 agccccgaaa gtgagatgcc cactcccata cccccctgga ttatagggac aggaattacc
    17521 agtccaccca tatttagttt tttcattgag atcattaaca gaataattta cgattgttaa
    17581 accccttatc cggaatggag taagtgcgct gtcctaaagc gccatgtcgt gccgtgaccg
    17641 aatgaaccac aaaagtagac ctgaaaaatc agtccttttg aaaaaagggc tgtactgcta
    17701 ttatagggcg gaatcgctgc aatgagggat gaaatgcagg tcggtcaggg atgaaatgcg
    17761 tcatgtttcg aggcaaaaca accttctttt taagaagccg gtaactaaac cttaaaattt
    17821 acttaccgga atggatgctc tttattttga agaaaccaat ttcctgaatt cggcgacatt
    17881 ctgccgcgaa acgggaatgc ggaaatcaag accttcggct tcgagcgtgt aattcgggga
    17941 aatgccggtc agttttaata cctggccgga attcaccaga cagcgccggt gacacttcct
    18001 gatacaggga aaagccgata gcttcaactc aacagcggtc aatgtttgcc ttaacagttg
    18061 ctttcgaacc acaccgccat cgcggtaaaa aatttcaata taattgctgg ctgattggat
    18121 taaaacaata tttctcactt ccccttcgaa aaactccttc ccgctctcag actcaatgcg
    18181 tacacgatca gtaatagcct gaagcggcga ttttcgttct tcagccaacc aatggcggcc
    18241 tgaatctatt gcatggacta atttttcttt cagagaacgg ttatagttga tcaggttgaa
    18301 aagcacaagg ggcaacagcg ccagcgctcc ggaacggaaa agtgtcatcg atttcagtcc
    18361 ggaaatccgg aataaaagag ccgatactga aaatcccacg gccagcacga cgatcatccc
    18421 tgtattccaa atcaattctt tacgtattgt ccaccggtcc ggattgaata gtttcggaaa
    18481 caggcccgga aaaatcatgg tacttaagaa gaatgtagcc gcactgacaa gccccatcac
    18541 caggacaaaa taaccatccc tggctgaagc aagaaaattt ataccaaacg gctgaaaata
    18601 cagcacgatc agtacaaaac ccatgcttac aaaacttatt gtgcgcaggt tatgtttgaa
    18661 gttattattg aaaggataag gctggttcag attttcaaac atacagtgta attcaggggc
    18721 aaatgtacga ttataaacaa gatcctgaat ctcaaccggc aacatgctcc aggctgaagg
    18781 ctgctgaccg ataggaatga ttctcttacc gatagggtca ggcaaggaag taagaatatc
    18841 taaaagctat cagttaattg aataatctta ccgggatttc ggtatctgta ttcgtttcta
    18901 tatcttagca aagaaaatat cctgcatgtt attctttttc taactgccgt tcttctgtaa
    18961 tttacatagc tcataatgaa tgctttaatt taccggccat gaaaacaaaa ttgctgacag
    19021 cttttaccat actcctccta ctcacaacat tcggccttca actctccggg caggaggcct
    19081 tatctgaaaa agatgcccgg atggcatggt ggcgtgaagc ccgtttcggg atgttcatcc
    19141 actgggggct ctattccatc cctgccggtg agtggaaagg cgacacggga catgccgaat
    19201 ggatccggac cactgcccgg attcctttag aagaatatga caaatttgtt cagcagttta
    19261 acccggtgaa attcaacgcc gaagaatggg tgaaggctgc aaaggatgcc ggcatgaaat
    19321 acatcgtact gacctcgaaa caccatgacg gtttctgcct ctgggattcg gaccagaccg
    19381 atttcgatgt gatgtcgacg ccgttcaaac gcgatatcct gaaggaactc gccgatgcct
    19441 gccgcaaata cgatatgcgc atctgctggt atcattccat catggactgg caccaccccg
    19501 attacctgcc tcgccgcgac tgggagaaag accgcagcgc cgaaggcgcc gacttcgacc
    19561 ggtatgttca atatcttaag aaccaaatcc atgaaattct gacgaaatac ggcgacattt
    19621 ccatcctgtg gttcgacggc gaatgggaaa gcacctggaa ccacgaccgc gcccttgacc
    19681 tatacaatta tatctgttca ttcaataaaa acatcattat caacaaccgc atcgacgtat
    19741 tccgcgaagg aatggcgggt ctgagcagta acgaaaaggc gctgggcgat tacggcaccc
    19801 cggaacagga aattcccgat acaggcttac cgggcgtcga ctgggaatcg tgcatgacca
    19861 tgaatgacca ttggggatac aacgccaacg accataactg gaaaacggcc gggcaactca
    19921 tttataatct cgtggatatc gcctcaaaag gcggcaatta tctgctgaat gtagggccca
    19981 cctccgaagg cgtattcccg cccgaaagca ttgaaaggct gaagactatc ggcaaatgga
    20041 tgaaaataaa cggcgcttcc atctacggta cacaggcagg gccttttaaa aacccggcct
    20101 ggggcaaatg cacgcaaaag gaaaccgaag gcggaacccg tctgttcctg cacattttcg
    20161 gcaatccgca agcaggaaaa atcaacctgc ccggtctgat gaatgaaacc gtaaaggctt
    20221 tcctgctctc cgacaaatcg gaaacgccgc tgaccattac ccgcaaaaac ggcagcgtcg
    20281 aaattaccct gccgcaatca gggctggatg aaatttgtac tgttgtagtc ctcgacatga
    20341 aagggaaacc ggaaattatc gacaagcctg aattctcttt ccgctacgat gccgcttccg
    20401 acaaggtcga gatcagcctc gtttcagctt ccacaaaacc cgatcttaat gtttattact
    20461 ccaccgacgg aaccttgccg accgataaca tgtccgtttt taccaaaccg gtcatgctga
    20521 aactaccggt aacactgaaa gctgttagct atatcgggaa aaaacgcgtt gggaacgtcg
    20581 ctgaggtatc tcttccggtt tcattcggta aaaccgttac cttggataac ctgccgtccg
    20641 aaaaatacag cgccggaggc cctgcatccc tgaccgacgg catccgcgga tcgaaaaaat
    20701 ttgccgacgg ccgctggctc ggatttgaag gcagcgatgc tgttgcctgc atcgacctcg
    20761 gaaaaaaaac agccattcga tctataaacc tgggatatct ggtcaacaca ggttcctgga
    20821 tctttgaacc ggttgaaatc acagtcgaaa cctcggtaaa cggggaaaaa tacacaaccg
    20881 ctctcacccg gaaatataat gcagccttat ggaaacaggc tgacggcatc agggagttcg
    20941 ctgatgtttt tcctgaagtg aatgcccgct atgtaaaagt aacggtaaag aaccggggcg
    21001 tatgtcccgc aacgcatccg ggtaacggcg gcaaagcatg gatttttctg gacgaaataa
    21061 gcgtggagtg atatttttca atctaacaga caatttttac cggcgctgcc ggaatttcca
    21121 ttaaaagcca tttaaaaaca agtaaccaat tcataatctg attctaaaat catttgctaa
    21181 ttctttcact atgaaaaatc tgttaacaaa atcattatct gttgtttttc tggcattcct
    21241 gctagcctcc tgcgacgatt ccggtaaaaa gtatgtcggc ctggaaccgg agcagatcaa
    21301 ggagaaaaac ttcaatggcg aaaaaaaaat atatacgccg gaagggaaac tcaggaccat
    21361 cgctgaatat aaaaacggga aagttgacgg aagagtccgg aaattttttg atgatggtaa
    21421 aatctacatg gatgcaacgt atcaaaacgg gttaaaaaac gggaaatgca cctattatta
    21481 taccaacggg aagccttaca ccgtgacgga ttatgttaac ggatacaagg aaggcgttga
    21541 aaaaacatac taccaggaag gcggattaat ggcggtggct aatttcagga aaaacataat
    21601 ccagccgggg ttacaggaat ataaaaaaga cggaaagcct attcccgtca atacaaccat
    21661 tatcatcagc gaagtgaacc acgtcagatt ggacggaacc ttccgtgtac agatgcgcct
    21721 ttcggataag agcgtcactc ccaaatttta tatcatggaa caatcggccc ctgataaaag
    21781 acaaaaagta aatatgtcaa acggcacggg cacactggtg ttgaatttca acgcttcaag
    21841 tttcgtgatg aaaaaactga tcatcgaagc cgagtataaa acctacaggg gtaatctcat
    21901 gaagattcga aaaccataca acctggctat tgacctgtag cgcattttac ttgtcaattt
    21961 gtttgagctt agctgtaagc atccagtttc cgggagaata caccaggcct ttgtttataa
    22021 cgcgtcgtgc ttttgtcata ttccctttcc tgaaataaca ggatgcagca tcgccatatg
    22081 cctctgcagg atcacaaaac accagttcct gttgcgacaa tgcctctgca tcggactcaa
    22141 acatggtcag atatttttcg ccggtggcta tctggttttc atcaaaagcc ttggaagcca
    22201 gcgatatggt cagatagcat cttcttgccc tgtaataatc cagttctctg atcccggggt
    22261 attccgaagc aaggctgtcc aatacttcaa agttccgttt atcggtataa ctcgttgaag
    22321 tgtaaaattc gaccagtttc gataatatta ccttcacctg catgctattt gcaacctgcc
    22381 tgtatgcatc atatactatg cggaaagctt tatctgtttc ttcattcatt aaaaggtagg
    22441 atgtgtaatt gacataatac aattccttaa gttttgactg taccagggta tcctggattc
    22501 cgtcgacgag agcattgtaa atcgtatgca gggaatcagg agtaggaaaa gaaccgaaca
    22561 aacactgatt gatcatcaac gagaattccc cgataacctg atcctcctta ttctggtttt
    22621 tggtatggtt gtaatattcc acaacatacc tggccctttc ggcggatttc aaactcattt
    22681 cagcaatcga agccctaagc agttgttctt tcattatcaa taattcatcc aggggggtaa
    22741 ggactatagc tttattcagc gcctgataag cttccaggga atgttgcttc atcgaagcct
    22801 ggaccatagc attaatatat tgatagccga ccagaccgcg aagattggta gtattaagtt
    22861 tcaggtaata ccggtcaaat atgtttccta ccggctgatt ttcaatatta tcggactgta
    22921 tgaatttgaa tagccgcaga ctctgcaaag tactggtgag tacagcatcg ttaagctgca
    22981 tgaaaccatg ttcaggatcg gtagtctcga acaggtaagg cgtttccccg ccataagcaa
    23041 tgagatagac atgtccgggc aacacttttg gggtgttcct gtagcccagt tcgtccagaa
    23101 tcaggctgta tagtatggtg gcagtaaggc agttataata acccgtctta aacattttag
    23161 agaatccggc atcaagatca taatgcttaa ggaaactggt ctgtacatgg ctgaagataa
    23221 tctgaagggc cttcactggc tttttgattt tttccgtttt catcttcagt atatctgttt
    23281 catttttaat catttgcctg atatcggcca gatcgctttc agtcgattcc ggatcaacgg
    23341 ctaccgacat cagcaggtaa tcaggctttt ttgaacaaaa tgattcaaag cctgttgatt
    23401 ccagctttgt agaaaacacc aatccggagc acgaatcggc aacgatttcc tgtgcaacta
    23461 ttcgaccgga cataaaaacc aacaggaatg gaatggatag aatgacaaga cgtaggttca
    23521 tatttatgag atatgttatt gaaacaacgg ttataaagca atagggatgc aaataatttc
    23581 aatccaaata tagtcttttt aacaaggcac gcaggttttc ttcttttaaa agttgaaatg
    23641 agtacttttg ctgctggaag caacgcgtcg ggatgaaaat atattttcac cgaactattg
    23701 gataatcaaa ttttattacg acctttgcac tccatttttg aaaaccccat tgccatgtca
    23761 cgagtttgcg aaattaccgg aaagaaagct cttgtaggga acaacgtttc ccattcaaat
    23821 atcaggacaa aacgtaagtt ttatccgaac cttcagacga aacgtttcta cattcctgaa
    23881 gaagacgaat ggattacctt aaaggtttcg gctgctgcca tccgcaccat taacaaaaaa
    23941 ggcatcagcg ccgtgttaaa aaaagtcagc gaaaaaggct acctgtagcc tcaacgatat
    24001 tcattgcgca aagctgctcc ccctcccggg agcagctttt tttatttacg ccggaacaac
    24061 cggctaaccc tcagcaggtt acgcggcatc accatcctat ttactattcg gcggacacga
    24121 tttcacccgg ccatgttcaa actttttcct tattcgctaa atctcagttc cgtgactatt
    24181 ttcattaaac gacatttaaa ttatctttgc agtcaatgaa caggaaatat gcttttataa
    24241 ttgcgctgtt gatggcaact gcctgcgctc aggcacaaca ccgcgatgac ggcctggtgc
    24301 agttctctgg tgtcattgta acagccgaca gtttacggcc tgttccattt acccatattc
    24361 tggtcgaaaa aacaaacagg ggaaccacat ccgattactt tggctttttc tccatcgtcg
    24421 ttcataaaaa agatattatt catttttcgg ctgttggttt caagaaggta gaattccgta
    24481 tccccgacac cctcagcgaa aaccgctatt cccttatcca ggttatgacc agcgacacca
    24541 ttatgctggc cgaaaccgtc atctatccct ggccgaccaa agaacaattc cgccaggcat
    24601 tcattaaact tgacgttccg gacgacgacc ttgaaatcat gcgcaaaaat cttgcttacc
    24661 ttgagctgcg cgaccggaga gataaaaatt acaatcccga cctgtacggg atggatgctg
    24721 ccatgaacta ccgcaactat gttgacaatt ataccaacaa aatgtattat gccggtcagg
    24781 caatgccaaa cagcctgttg gatccgcttg cctggttaaa attcattaaa gcctggaagc
    24841 ggggcgactt taagaaaaaa tactgattcc atatgctacg gtaacatacg atattccggt
    24901 aactaccgtt aataaaggta atccaaccag caccaaccct ataactgact cattacattg
    24961 gaccgggaac attttcatca gaaccgttgc tacttctcac ccctcctgct gattcttttc
    25021 ctggggtttt atctgcccgg gcaatcccaa acgtttgcca aaatttacgg caaagtaacc
    25081 gatgaaagaa ataatgccat tgaaatggca aatattgctg tatcaggata tccgggagga
    25141 gttacatcgg ggaaagacgg cgcttacgaa cttcgcgttc ctgccatgaa accgcttatc
    25201 attatatact cattcgtggg ctatgaaagc gacacattac gcattaccct ggatgccaat
    25261 ataaatttcc gggccaacaa ggttctgaaa cagaccgcca tgcttctgcc ctacctggtt
    25321 gttgaagaca aagccttgcg aaatacaaac ctgagccgca tcgatccgca cacagcagcc
    25381 atgatccctt cggcatcagc cggcattgaa tcgctcgtaa aaacgatgcc cggtgtggtg
    25441 tcgaacaatg agttgagctc gcagtactct gtccgcggcg gtaattacga tgaaaacctg
    25501 gtatatgtca atgatataga aatttaccgc cctttcctga tgcggtcggg acagcaggaa
    25561 ggattgagtt tcctgaacag tgacctggtt tcatcggtgc tgttttcagc cggcggcttc
    25621 gacgctaaat acggcgataa aatgtcaagt gttcttgata tccggtacag gaaaccctcg
    25681 gaatttgcag gctcggcctc ggccagcttg ctgggcgcca ccgggcatat cgaaggcgtt
    25741 tcgaaaaaca accggttcac ctacctggct ggcgtccgtt acaaatccaa ccagtatctg
    25801 cttaactcgc ttgagactaa aggagattac aagcccaggt atttcgatat tcagagcctc
    25861 ctgacttaca atgtcagccg caaggtcgaa gtatccttcc tgggatatat tgcccgcaac
    25921 gattacacgc tgataccgca atcacgggaa acaaatttcg ggaccatgta cgacgcgctg
    25981 aggctgaagg tttatttcga cggacgcgaa gccgataact accgcagtta tttgggagcg
    26041 acaaccgtta cctacaaacc tacagagcat ctgaatataa aatggatagt atccggttac
    26101 cggtcgcagg aaaatgaatc atttgacatc ttagggcaat attggatcca gttgctcgaa
    26161 aacgcccccg actctatcga agtggttgaa aataaaggtg tcggatcata tcttacccat
    26221 gcccgcaatg aattgaatat ggatgtgttt aacattgaga accgttatac attgatccat
    26281 gacaacagca atacccagtg gggcgtaaaa taccagcatg aaatcatcca ggaccaaatc
    26341 gacgagtgga tgctcgtcga ttctgcagga ttttcaaccc cctattctcc gggtataccc
    26401 ggaatggcgg gcaacctgac tgacctccgg ttatattcct gcctgaagac ctcggccgat
    26461 atcacttcga acaggtatac gggatttatc cagaacaccc gggacctcga cggcgattcc
    26521 acgaagctga cacttacaac aggcctgcgg ttcagctatt gggactataa caggcaattt
    26581 ctggtcagcc cgcgggcgac aatttccttt aagcccaact ggcaacgcga tatactcttc
    26641 aggttttcga caggatacta tttccagccg cctttttatc gcgaattgcg aaacctggag
    26701 ggacaggtta atccggaagt ccgggcacag aaatcaatcc atctcgttct cggcagcgac
    26761 ctcaatttcc aggcctggga ccgccctttc aaatttgtta ccgaggtata ttataaaatc
    26821 ctgaaagacc tcatccccta cgaagttgaa aatgtgagga ttcgatatct cggttacaac
    26881 gattcgaaag gatatgcagc gggcatcgat atgaaggtaa acggcgaatt tgtgaaaggg
    26941 gttgaatcct gggccagcct ttccatcatg cgcaccgaag aagaccttta cgacgattat
    27001 tattacgaat attacaacca atccggggag aaaataattg ccggtataac cacggaccaa
    27061 acggttaccg attccgtgaa ggttgaaccg ggatatatcc cccgtcctac tgatcaacgg
    27121 gttacattcg ggctgttttt ccaggatttt ctgccgatga atcctaccta taaaatgaat
    27181 ctcgggcttt acttcgggac agggcttact accggcgcgc cgaatacgcc gctgtacaag
    27241 catgttttcc gcatgcctgc ctaccgccgt gttgatatcg gtttcgccaa acagatcatc
    27301 ggtcgtgaag ccggccaatc atcccgcaaa tttttcggaa acattaaaaa catgtgggtg
    27361 accgcagaga ttcttaacct gctgcaaatc aacaatacca tttcctatat gtgggtgcgc
    27421 gatttcgacg gaaatcagta tggcgtaccc aattacctga cttcaaggca gataaacatc
    27481 aagctctccg tggaatttta acggcccggc tgtctgaacg tgaatatccg gcggcagcct
    27541 gtctgaatgc ccttaaccgg aaagctgaca aattcctgat tttttatctt tttcatccaa
    27601 ccgggcgaac tatttcgtcc ggaaaatgtt atttatatta aaacttaata acaccattgt
    27661 accatgatat atcaattccc agaattacca tacgcatatg atgccctgga accatacatc
    27721 gacaggatga ccatggaaat acaccatacg aagcatcata aagcctattt cgacaaattt
    27781 cttgccgctg taaaagacac cggatttgac ggaaagcctc tggaagagat catccgcaac
    27841 gccgggaagg cgcctgccgc tgtccgcaac aacgggggcg ggttttataa tcattccctg
    27901 ttctgggaag tgctgtcgcc aacaggtggc ggaaatcctg caggcaaact tgccgacagt
    27961 cttagaaagc attttggcac gtttgatgaa ttcaaagccc gtttctccga cgccgccatc
    28021 aacaggtttg gcagcggatg ggcctggctc agcgtgaaat ccgacggcag cctttgcgtt
    28081 tgctcaagtc ccaaccagga taatcccatg atggatattt ctgagtgtaa cgggatcccg
    28141 attcttgcgc ttgacgtctg ggaacatgcc tattacctca gatatcagaa ccgccgcccg
    28201 gaatatgttt cagcattctg gaatatcgta aactggcaaa aggtgaatga aaactacgag
    28261 aaggttttgc ttactctata aaacacggat ttgtgtttgt ttggttggtt gctatgggag
    28321 atgccgccgc tgcggcatct cccggttttt aatagctgat ggatcataat aaaccatgtc
    28381 agcaggtatt taccatttcc cgcacaacaa tgaagttcaa aatttattcc taacttcaaa
    28441 cctaaaacta aagatcccat gaaaacgcag ataacctttc tcagcctttc gttccttctg
    28501 tgcatttcgg cttcagccca gtacaattac ggcaaccagg caggaatgct tacaactgga
    28561 gatttaaaaa tatatgccca ggatgccgcc gggcatcaaa ccggcttccg gtttccggcc
    28621 ctgccctata cctataacgg gctgcagcct gctatcgaca gccttacggt agccattcat
    28681 tacaacaggc accaccgggg atattatgaa aactttgtcg ccaccataaa gggaacgcca
    28741 ctcgaaacca tgcccatcgg agaaattttc gcagagatca gcaggcaacc ggttgccgtc
    28801 aggaacaatg ccggcggtta ttacaaccat ttcctctatt gggaaaacat gcagattaca
    28861 accggaaata tgccgtccgg cgacctggaa gccgccatca tcagaaaatt cggatcggtc
    28921 ggcgcattca gggaacaatt cggaaacgcc gccaaagcaa tcttcggcag cggctgggcc
    28981 tggctcatcc tgaataagga taaagaaccc gaaatcacaa ccacgtccaa ccaggataat
    29041 ccgctgatgg atattgcgcc ggttcaggga atccctttac tggcgcttga tgtatgggaa
    29101 catgcctatt atctcagcta ccagaaccgg cgggctgatt atgtaaatgc tttctggaac
    29161 catatcaact gggacgaagt agagaggcgc ttttctgaag cgatgaaaaa gtagaaggca
    29221 ataacagcgc tcccggggaa tcgggttatt ttatcattgc cacggcgtat gccgacacgc
    29281 cttcgcccct gccttcaaaa cccattt
//
LOCUS       DAOPYV010000038        28336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_1659_length_28336_cov_11.340122, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000038 DAOPYV010000000
VERSION     DAOPYV010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 28336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28336
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1659_length_28336_cov_11.340122"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..4448)
                     /gene="glgP"
                     /locus_tag="PKI35_06585"
     CDS             complement(210..4448)
                     /gene="glgP"
                     /locus_tag="PKI35_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNX44088.1"
                     /translation="MNQNHHTKPDYIFEISWEVCNKVGGIYTVVSTKAPGIQKEYGDN
                     FILIGPDVWKETRQNPYFTEDPDLLRSWRKTAESEGLRIKIGRWNIKGNPIVILVDFT
                     MFFSSKDKIFAQFWETYKLDSITGNWDYVEPALFGYAAGKVVQSYYEFYLTAQDTLVA
                     QFHEWMTGAGVLYLRNNVPQAGCVFTTHATVVGRSIAGNNLPLYSHFDEYDSGEISRR
                     FNILAKHSLEMHSALVSDAFTTVSEITARECVHFIGRVPDVITPNGFDEELIPEKAEL
                     EAKRESGRKRLLDVARAVLNQDLPPDSLLLVTSGRYEFRNKGVDLLIKSLGNLNRENK
                     LKKPVVAFILIPAHHYGPVAGLNERIGSPDFQSPNTGKYLTHGLFDPHDDIILNTISE
                     CGLNNSVQDNVKVIFVPSYLVGTDGVINIGYYDLLPAFDLSVFPSYYEPWGYTPLESL
                     AYGVPTVTTSLAGFGLWARKNCGNEVINVIDRNDENAVEVTDEIVKVIRKFDDVDEET
                     RNYYIVHAIELAKKALWTNLESQYRKAYAIALQKVASRAELYRDKRHAEVMEELPVKP
                     HSVPEWKKVMIRQSFPPSLEPLNILSYNLWWSWNSEAVELFYSISPELWKKYDKNPIP
                     VLNALSYQQLRKLESDKAFIRKMKKVYADFEAYMDEGNRKSGPCIAYFSMEFGLHDSL
                     QIYSGGLGILAGDYLKQASDSNTHMIGIGLLYRYGYFKQLLSPYGEQISQYNPQRFNL
                     LPISPVRTKDGNYVQVQIALPGRNLIAKVWRVDVGRVPLYLLDTDIDENHESDRTITH
                     QLYGGDLENRFKQELLLGVGGIRLIEAIGANPDIYHCNEGHAAFIGIERLRIFVHELK
                     FSFAQAMEIVRSSSLFTTHTPVPAGHDAFPEDILRTYIPHYAERLKISWNTFMNLGRY
                     IENKPDEKFSMSVLAARLMQEMNGVSRIHGRVTRAMFKDMYDGFFENELHIDYVTNGV
                     HLPTWAAHQWKQLYKNVFGDNFLNEQSDFKSWQKIENVEDQVIWDIRQQLRGELIDFI
                     TRRYQEEMSYSHENPKHIIKIIDTLDRNVLTIGFARRFATYKRAHLLFSNLERLSEIV
                     NNQGREVQFIFAGKAHPADKAGADLIKRIIEVSRMPQFVGKIIFLENYGMDMAKKLVQ
                     GVDVWMNTPTRPLEASGTSGEKAIMNGVLNLSVLDGWWAEGYIPGAGWALKEERVYQN
                     QQFQDELDAETIYNLLEDDIVPTFYDRGPDDVPHKWVSHVKKTISDISPHFTMKRMLD
                     DYHEKYYNKLFKRTRMMRADDYLMPRKLAKWKMKVARNWETIELVDYNNPDSGKPLNL
                     GDSFNAAITLKLGELSPDDVRVELVAGQRTNEVMDEPTFIREFTFQKKEGANSIFTLG
                     FSLTLAGVFDYAVRITPKNQMLPHRLDFNLVKWA"
     gene            complement(4712..6082)
                     /locus_tag="PKI35_06590"
     CDS             complement(4712..6082)
                     /locus_tag="PKI35_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNX44089.1"
                     /translation="MRSICLYFQVHQPFRLRTYRFFDIGADHYYYDDYQNKHIVRRIA
                     EKCYLPANEILLSLFREYGNDFRVAFSITGTSLEQFKKYTPEVIESFRKLAKTGNVEF
                     LAETYSHSLAAFNNETEFRRQVEQHARTIGKLFGVKPVTFRNTELIYSNDIADMVYKL
                     GYHTMLTEGARQVLGWKSPNFLYNSRNHPGLNLLLRNYQLSDDIAFRFSQKTWSEWPL
                     TTEKYLNWLNAIDKDQKVVNIFLDYETFGEHQKAESGIFSFLKALPGTVLKSGKWNFH
                     TPSQISELLDPVASLDVPYTISWADEERDVTAWLGNDLQDEAFETLYSVSDIMAECDD
                     EGLLRDWNYLQGSDHFYYMCTKWFSDGAVHQYFNPYSTPYEAFINYMNVVSDFLIRVR
                     EYEEARSAGGHTVAKQKPDVARPLKEKTGKKEATSVTAVPERTAKTKPDKLTDKKAVT
                     RRKAAR"
     gene            complement(6103..7389)
                     /locus_tag="PKI35_06595"
     CDS             complement(6103..7389)
                     /locus_tag="PKI35_06595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX44090.1"
                     /translation="MRVLMFGWEFPPHITGGLGTACFGMTKGLLKHGVEVLFVVPKAY
                     GDESQEAVRLINASDVTVKMQEPAFMEYWNKITFMEIGSNLIPYLDPEEFEKAVEEDL
                     RTTEEKVNPVFAERFSFSGKYGHDLMAEVSRYALVGAALAAEQHFDVIHAHDWLTYPA
                     GVAAKRVSGKPLVVHMHATEFDRSGENINKNVYDIERNGMEEADRVITVSNLTRNIVI
                     ERYGIAPDKVFTVHNAVEPVEKGELADCEKHVKEKVVTFLGRITFQKGPEYFIEAAKK
                     VLDRDPNVRFVMAGTGDLMEKMIRRAARLKIASKFHFTGFLKGENVDRMFAMSDVYVM
                     PSVSEPFGISPLEAMRSNVPVVISKQSGVAEVLKHVLKVDFWDIDAMADAIYSLLHYN
                     ALTTMFRKLGTEEVNSLKWETAAGLIADVYEQVYQK"
     gene            complement(7408..9369)
                     /locus_tag="PKI35_06600"
     CDS             complement(7408..9369)
                     /locus_tag="PKI35_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNX44091.1"
                     /translation="MSYIQFTKDQLVNLEFSLTRELLRSNRAGSYACSTIIGCNTRKY
                     HGLLVAPQPQIDGGMHVLLSSLDETVIQQNAEFNLALHRYMGNVYSPKGHKYITDFVS
                     DPLPTVTYRVGGVILTKEMIFIAGDDRCIIKYTLVDAHSPTTMRFRPFLAFRNIHAVS
                     KANGYADKDYHEALNGIYVRLYPGFSNLYMQFSKKPVYTHHPDWYFNFEYIREKARGY
                     DYLEDLLSPGTFELPIDKGESIYFAVSLEEANPESIIRIYEDEVSEHVHRDSFEHCLL
                     NSARQFILHRGKHTEILAGFPWFGRMSRDTFIALPGLTLLQKDYRTFKAVIDSLVAEM
                     KGPLFPHMGSGISAVYNSVDAPLWFFWTLQQYMLLSGDKNVWKYYQLPMKTILYGFRQ
                     GALFNIHMLDSGLLYAGQEGTAVTWMDAIVEGQPVTPRIGLPVEINALWYNAIMFALE
                     LARKNGDSQFVKDWEEIAEKIPDEFVRTFWIADKNYLADYVYGDYRDKAVRPNQLFAA
                     SLPYSPLQEEQCKSVIDRVTSELLTPRGIRTLSPINPAYKPEYTGNQAERDRAYHQGS
                     VFPWLLGHYAEGYLKLYEESGVGFIQKIYAGLEEVMTEHGIGSVSELYDGDPPHKPSG
                     AISQAWSVAEVTRMKWLLDQYKQEKKSKK"
     gene            9600..11069
                     /locus_tag="PKI35_06605"
     CDS             9600..11069
                     /locus_tag="PKI35_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNX44092.1"
                     /translation="MMTFPVIPKKISTWSLFILAGFAVMSGCRKEEFNTDKNYMLKFS
                     SDTVIFDTIFTSVGTTTHVLMVYNSGSLPVKISSIRLAGGSASPFRINIDGEAVSQKS
                     DVEIAAKDSLYIFVKVTIDPNDADGPFIKADSILFETNDNVQDVKLIAWGQNAYFYHD
                     TVLTGNIILKNDKPHVIYGDLIVDSLSSLTIEEGAQLCFHRNSGLIVKNTGKLKVNGT
                     LADPVVFRSDTYVNFDPLHDTVPGMWSGIRLQSGSGRHELNFAEIRNAEAGIVIDSAD
                     ASLAPVLTLYNCKIRNTVSYGISAVASSVLAANCEFSNCGGFLLAIQQGGNYDFRHCT
                     FANYWQWLRKTPSIYLSNIYYGLLGEKHLQALEKAYFGNCVIYGNYRVDTTRTELTVS
                     EDKSTPFSFTFDHCIIPSGLNKNYPDSFHSCLVREDPLFLDTDHWNYQLDTLSPAKDI
                     GLRSIIDFSSLNLTSDLNGNSRVSDTGPDLGAYERIENR"
     gene            complement(11186..12067)
                     /gene="sucD"
                     /locus_tag="PKI35_06610"
     CDS             complement(11186..12067)
                     /gene="sucD"
                     /locus_tag="PKI35_06610"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNX44093.1"
                     /translation="MSILVNKDSRVIVQGSTGSEGTFHATQMMEYGTTIVGGVTPGKG
                     GQTHLNLPIFNTVREAVKATNADVSIIFVPPAFAADAIMEAAEGGISVIVCITEGIPV
                     KDMLMVKEYLRGKDCRLIGPNCPGIITAGETKVGIMPGYIHKKGIVGVISRSGTLAYE
                     AVDQLTKAGLGQTTGIGIGGDPIIGTSIRDAAEMFMADDETKGIVIIGEIGGNMEAEA
                     AFYIRDHGTKPVVSFIAGATAPKGRTMGHAGAIVGGKADTAQAKKAIMRECGITVVDS
                     PADIGTAMLKLMGRPAV"
     gene            12555..13805
                     /locus_tag="PKI35_06615"
     CDS             12555..13805
                     /locus_tag="PKI35_06615"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNX44094.1"
                     /translation="MVPCSSFNKTLSGRRIISIFLLFAVIISWSSCSRRKKEVAPADV
                     RNCIDLRFPSKKEEASIRREINADLKSRRLDSLFREKARLRGFNGAVLIAQRGVIIYK
                     NAFGYTGPDRKTPLTTDHIFQLASVSKTFTAIATLMLAERKALSLDDSVQKFFRDFPY
                     HGIRIKDLLSHRSGLPNYIYAFDEWRNTVTVPPTNSTIMRWFAVSSPPPVPYNLPDVA
                     FSYNNTNYIILASIIAKVSGKTYAEFLKTEIFEPLGMVHTFVDTLCPVSLKALKTFGH
                     DYNRRRDRDFFDGVYGDKGIYSTVDDMMRWYMALSSHCLLSRHLLKEAFTPRSLEHRS
                     RHNYGYGFRLITDPGNMNKVEYVYHGGWWAGYSSMILFDLRDDYVVIVLSNRKNTSVY
                     ENKNVMDILDDKPTDENEEEIDSI"
     gene            13946..15331
                     /gene="mnmE"
                     /locus_tag="PKI35_06620"
     CDS             13946..15331
                     /gene="mnmE"
                     /locus_tag="PKI35_06620"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNX44095.1"
                     /translation="MDDTICALSTPPGMGAIAIIRLSGNGALDVLSSVFRPLKKSIKV
                     PEIQSHTITLGTFGAGNETLDEVLVSVFRAPHSYTGEDVVEISCHGSVFIQQKILETL
                     TSSGARMARAGEFTLRAFLNHKFDLTQAEAVADLIASNAKSSHDLALSQMRGGFATKI
                     KNLRQRLVDLASLLELELDFSEDDVEFADRRQLLDTIESLDDELSRLAGSFKLGNVIK
                     QGIPVAIVGKPNVGKSTLLNALLNEERAIVSEIPGTTRDTIEDTLTIKGVSFRFIDTA
                     GLRLHSGDIIENLGIGRTYETMQDATVILYVFDMSQITADEIDELLEEFREHIDDPEK
                     HFIMVGNKTDLMVEVPPHVARLFDIETVFISAKRKENINLVIESLLRAVNINQLADST
                     IVSNARHHEAITKTLSALESARQAIENNTTADLVAIDIHSALYHLGTITGEVTTNEIL
                     GNIFGKFCIGK"
     gene            complement(15352..15858)
                     /locus_tag="PKI35_06625"
     CDS             complement(15352..15858)
                     /locus_tag="PKI35_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNX44096.1"
                     /translation="MKKTGIFYSKNAVITSEAAKKIQDAFGDFRIEVVPVETAWQKDF
                     ERFDNIIAGASTWFDGELPAYWDELIPELNTLNLKGRKVAIFGIGNQADYPENFVDGI
                     RILADAFEVAGARLVGQTSVEGYSFERSRAVVDGQFLGLVLDVESQPEKTDQRIRLWV
                     ETLKKEFQ"
     gene            complement(15872..17905)
                     /locus_tag="PKI35_06630"
     CDS             complement(15872..17905)
                     /locus_tag="PKI35_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNX44097.1"
                     /translation="MKKFDILSTDKETLKKWYFLMALGRAIDEKAPVYLLQSLGWSFH
                     APYAGHDGIQLAIGQVFTRGEDFLFPYYRDLLTALSAGLTAEEIILNGLSKVTDPTSG
                     GRHMSNHFAKTEWHIENISSATGTQDLHAVGVARAMQYYGHKGVVITSHGEATMSEGF
                     VYEAVNGASTERLPVIFVIQDNGYGISVPKKDQTANRKVADNFSGFKNLKIIHCNGKD
                     VFDSMNAMTAAREYAFNTGNPVIVQANCVRIGPHSNSDKQGLYRDDNELSYVKAADPL
                     QKFRRMLLRYNRFSEEEIKQIEDDAKVQLSIAGRKAIAAPDPDPSTVLDYVLPEPYRP
                     QKYTDGTHAGEGEKKNLVTAINETLKAEFRHNPDTFLWGQDVANKNKGGVFNVTKGMQ
                     QEFGEKRVFNAPIAEDYIVGTANGMSRFDPKIHIVIEGAEFADYFWPAVEQYVECTHE
                     YWRSNGKFTPNITLRLASGGYIGGGLYHSQTIEGALSNLPGARIVYPSFADDAAGLLR
                     TSIRSKGFTLFLEPKALYNAADAATFVPDDFEVPFGKARIRLPGDELSIITYGNTTHL
                     SLKVARRLKAEENRSVEVIDIRSLIPLDKETIFQSVKKTNKVLIVHEDKVFSGFGAEI
                     AAMIGSEMFRHLDAPVQRVGSLFTPVGFHHDLEKAILPDEERIYKAARELLLY"
     gene            complement(17935..19269)
                     /locus_tag="PKI35_06635"
     CDS             complement(17935..19269)
                     /locus_tag="PKI35_06635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNX44098.1"
                     /translation="MPTFDIKMPKLGESITEGTIVSWLVKAGDHVNEDDVIFEVTTAK
                     VNAEVPSPVSGILIKILYSEGDTVPVGNVVAVIQPDGIDEAEASDKAGSLDSTMESPG
                     SDASFQEPLTGPAVKTEKGIKDDRWYSPVVMELAKEAGIGAEELSQVPGTGYLGRLSN
                     KDIKEYIEQKKKGKMPFASKDKLPIEAALSAPPIQNRPVPAPVLVTESADDYSETVNM
                     DPVGRIIADRMIQSKNVSAHVTTVVEADVTRLVKWRDSRKDSFMKQEGVALTFMPAIV
                     NATAMALKEFPKLNASVSDYKFILKKHINVGVAVALDDGNLVVPVVHDADKLNISGLA
                     LAIDSLAKKARTNKLTLDDVQGGTFTITNFGTFRNIIGTPIINQPEVAILGVGYIGKK
                     PAVIETPEGDAIAIRHQMYLSLSYDHRIINGAVGGAFLRRIADFLENWDTNS"
     gene            complement(19301..20155)
                     /locus_tag="PKI35_06640"
     CDS             complement(19301..20155)
                     /locus_tag="PKI35_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HNX44099.1"
                     /translation="MRNDNETKLRQPAGDMADISENEIPKMLCIVNDQTDAYFNMAAE
                     EYLIRNFSTPVFMLYQNEPSVIIGRHQCVDAEINTDFVREKQIKIVRRNSGGGAVYQD
                     YGNLNLSFMETGNSADFDKYAKQMQHLLSTLGIHAQTDARRALYVDGLKISGSAQFVR
                     GDKTLYHATLLFSTDLINMASALEGRYADFENMGPTERNRYVKSVRSRVTNIRDYLPM
                     PMTLSDFKNHIFQYFLRYNKSNSLYRFSREDIRAIENLRRVKYATSAWNLQDGLSFNQ
                     KIGTINAC"
     gene            20383..21183
                     /locus_tag="PKI35_06645"
     CDS             20383..21183
                     /locus_tag="PKI35_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44100.1"
                     /translation="MKQIKEFITIIILLSTLLPAFSQSGYSVEYNHKYIWKDEGIDME
                     ITVKGEVPFHTGTNFAVIPKCQTGSHDRLDSVKVVSILEGEGEVMAKGKSILGDKSDP
                     ENLVVMEYTLPVKIKITGKTWYKDVTCAEMWNLALEEDWTGKVEWNYQAKSPEAAAVA
                     QALSPATFGQMNRLEDHTLEFQRYPVFSDKPLVLIEDAFPDMGFPMKGRLLFRVNIPG
                     IHRIGIDEEYDWDESIQPNKEYKFSDLHWGPPLDQINWNVTDVDKIRN"
     gene            21611..22504
                     /locus_tag="PKI35_06650"
     CDS             21611..22504
                     /locus_tag="PKI35_06650"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX44101.1"
                     /translation="MYTVDILLPAYNGEQFLKEQIESVLSQSYPEIRLYIRDDGSTDG
                     TGELLRAFAASDKRVTLVEEPAGNLGLIKSIECLLSHSESRYIMFCDQDDVWCPNKVM
                     EMLNAMLKQELKSEPGVPIMVHSDCFITDHRLNIRGRLLGNLPARSGLRHALFTYMVQ
                     GASMMINLALKDVILPFNGYVYLHDRYIHLVSEITGKRVYLDYPLMYSRQHNYNFLKE
                     RSFLHKISDYIFRPYYTYFTQYDRNLIQSLSERFPGNKLLNIYLDITSDKISKFRKIK
                     LLASNHISMKPKYLILFLIKG"
     gene            22923..23687
                     /locus_tag="PKI35_06655"
     CDS             22923..23687
                     /locus_tag="PKI35_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44102.1"
                     /translation="MSKHLYLTTSPESLIASMLPPVEFGTYLSRGTKNRNKGEAIYFE
                     IDLFQVEKLIDLDSLNRRCVAKPDGSPKCSVYLSIYRVLEKLPLSAFKNLYLTTENGH
                     TLELKKSPYNSGKEVRGKLHLYQELCPVTPMVASILAPSAFLKRLTDGSSPIVLPKLL
                     FVDLKLGELATNPLRGSAEQLPYPNVGHLRDCLEILREEEGKEMKTVLRFFFGLLLFR
                     TIESGFFIGAKDEMIYYPYPSISELELINYDFFRSI"
     gene            complement(23866..26253)
                     /locus_tag="PKI35_06660"
     CDS             complement(23866..26253)
                     /locus_tag="PKI35_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX44103.1"
                     /translation="MLINKTIFHFRRSPKLIYTLLSFAGFTVGITAGLLIYLWVFQEL
                     QYDKFHPDFQNIYRVLTLSKQNDAVRKSAASYRPLAGTLKRDYPQIEFAAYISYSSED
                     SPLKTGNSSSFIEARQASVNQDFFKVFRGFEFLEGNPDALYLIPDQIVISKKIKEKLF
                     GKTPAIGKTIISEKYSKKIYTVGAVVDVPDNSSLQFDYLIPETGDNYNNQWADSYWTR
                     VYIKLAKNAVINDDFKHAIANQIQKYLPIKDKLLFQPLADIHLKSDFDDMLVGNPGNI
                     KYLWVLSLLGGFILFMAAFNFSMFSITSSSERSKEIGIRKVNGASRMQILFQFLKETF
                     FQTFSAAALAMILLCLLLPAVNDMTGLSIRIILSFRLIISLIALTALLSLLAGLYPST
                     CLSALSPVKIIKGGADTGSKAGLIRTLVIVQVSFAAFFLIVTAFLIKQLEYVRTKDLG
                     LQKENIVVIPTGLWYDSDTFKDELLKNTDIKAVSASAYAPVDFSWQVSLPLNSLGKSD
                     SVRMSMIMADQDFAKTYDLKILKGEFIPTKYADYWAARKKKSKAGNDDADDEVSMPVV
                     INETAEKTLGIPDIVGKRINEKLIVGVVKDFHFRPLNYPIEPVMIMNDPENIMTMNVS
                     ISPHNRQATIEYVRKVYKKYRDGREMSYMFFEDMLQDKYKAETRLRNITLGLGLAALI
                     ISLLGITGMVLFTVNRRIKEIGIRKVNGARTYDVLLLLNKDFIMWAFVSVIIATPVSW
                     LSVQFWLRDFAYKTPISWWVFALTGICILIIILFTVSRQTWKAASRNPVEALRYE"
     gene            complement(26260..27741)
                     /locus_tag="PKI35_06665"
     CDS             complement(26260..27741)
                     /locus_tag="PKI35_06665"
                     /inference="COORDINATES: protein motif:HMM:NF027611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4933 domain-containing protein"
                     /protein_id="HNX44104.1"
                     /translation="MKFEILLIALLLLLVGETSCNRNRLKMDEQSLVKELADEQKSGV
                     WQNGQATGSFSNAKPGIRYKEIRSADPSNPPVVIDIAAVQDHAAKIKASRLFQQIQVV
                     RIPADETKSPLSHYSKNYIISNRHIYQFSRFTGIRQYDMRGRLVKDICDNNMPYIDNG
                     EGSVAVDAREYKQQFSGAVSARLVDGKFYYQYENQPGDQCELKMVDDADNAPSISDNR
                     TGVENHRNFEGFGKTVIDFGKPSGNGINPNLYCILPGNQLATYSSAKSPAGNAPLLTL
                     LTKNGDTVCIFNDYDPVKHLKGRVYRSPDENYMYPVDNQFHIKPAFNDTIFELLPPNR
                     LLAKFVLHFGDRGVNNSTEGMDPQFNLKDKLLLNDLIESDKYIFITYTRDYSCPNTAK
                     HGTLKYGRIIFDKSKHEVIAVYSCEKPEINPEQPFSWPKAPEKNIENDLNGCPFFWPT
                     GVTADNKPFAAVSGKRLMRYFRPGQAAPMETLQENDMVIFIYK"
     gene            complement(27774..>28336)
                     /locus_tag="PKI35_06670"
     CDS             complement(27774..>28336)
                     /locus_tag="PKI35_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX44105.1"
                     /translation="VIFANKSFSSNFIISFDSAHQKEALATVNSVWKKMFPGLPLEYT
                     FISDSYRSIYSVEYNQSRVLSLFTIISVILSALGVFAVASFNIQQRTKEIGIRKVNGA
                     KISDVLLMLNKSYIKWMSIAFAAAVPVTYFAMTKWLENFAYKTPLSWWIFALAGFLAL
                     VIALLTVSWQSWRAATRNPVEALRYE"
BASE COUNT         7298 a         6936 c         6255 g         7847 t
ORIGIN      
        1 ttcgtggaat tcgtggctca ttattcgttc tccgttctcc cctgcctgcc acccgcaggc
       61 ttacgcaagg caggcaggcc tgcctgcccg ctcacacctc tccgctggca ggtaaagttc
      121 tccgttcttc tttcttcttt ctccgtcctc cgttcattcc atgatcagta acatatttcc
      181 attaagattt accacacttt cattgctctt tacgcccact tcaccaggtt gaaatcaagc
      241 ctgtgcggca gcatctggtt tttgggagta atgcggacgg cataatcaaa aactccggcc
      301 agcgtcaggc tgaaaccaag ggtaaagatt gaattggccc cttctttctt ttggaaggtg
      361 aattcccgga taaaagtggg ctcatccatc acttcgttgg tccgctgccc ggcaaccagc
      421 tctaccctga catcatccgg cgacaattca ccaagcttga gcgtaatggc ggcattaaaa
      481 gaatcgccaa ggttaagggg cttcccggaa tcaggattgt tgtaatcgac caattctatt
      541 gtttcccagt tgcgcgcaac cttcattttc cacttggcaa gtttacgcgg catgagataa
      601 tcgtcggctc gcatcatcct ggtgcgcttg aacagtttat tgtaatattt ttcatgataa
      661 tcatccagca tccttttcat ggtaaaatgc ggagagatat ccgaaatggt tttcttaaca
      721 tgcgataccc atttgtgggg gacatcatca ggcccgcggt cgtaaaatgt aggcacaata
      781 tcatcttcca gcaggttgta gatggtttcg gcatcgagtt cgtcctggaa ttgctggttt
      841 tggtaaaccc tttcttcctt cagcgcccag ccggcgcccg gaatataacc ttcggcccac
      901 catccgtcga gaacgctcag gtttaagacc ccgttcataa tagctttttc gccgctggta
      961 cccgatgctt cgagcggacg tgtcggggta ttcatccata catccacgcc ctgcaccagt
     1021 ttcttggcca tatccatgcc ataattttcg aggaatatga tttttcccac aaactgcggc
     1081 atccttgata cctcaatgat ccgtttaatc aggtcggccc cggccttgtc ggcaggatgc
     1141 gccttccctg cgaaaataaa ctgtacctca cggccctggt tattcacgat ttcggacaga
     1201 cgctccaggt tggaaaatag aagatgggcc cttttgtagg tcgcaaaacg cctggcaaat
     1261 ccaatggtaa gtacattccg gtcgagcgtg tcgatgattt tgatgatatg ttttggattt
     1321 tcatggctgt agctcatctc ttcctggtaa cgccgggtga taaaatcgat cagttcgccc
     1381 ctgagttgct gcctgatgtc ccagataacc tggtcttcaa cattctcaat cttttgccac
     1441 gatttaaaat cggattgttc attgaggaag ttatcgccga acacattttt atataactgc
     1501 ttccattggt gcgcagccca ggttggaagg tgtacaccgt tggtcacata gtcgatatga
     1561 agttcatttt caaaaaagcc gtcatacata tccttgaaca tggcccgcgt tacccttcca
     1621 tgtatacggc tcaccccgtt catttcctgc atgagcctgg ctgccagcac gctcatcgag
     1681 aacttttcat ccggcttgtt ttcaatatac cgtcccaggt tcatgaatgt attccatgaa
     1741 atcttaagcc tttcggcata atgggggata taggtacgca gaatatcttc ggggaaagcg
     1801 tcgtggccgg caggaaccgg cgtgtgggta gtgaaaaggc ttgatgaacg gactatttcc
     1861 atggcttgcg cgaatgaaaa tttcagttca tgaacaaaga ttcttaatct ttcgataccg
     1921 ataaaggcag catgcccttc gttgcaatgg tatatgtcgg gattggctcc aatggcttca
     1981 atcagcctga tccctcctac cccgagcaac aattcctgct tgaaacggtt ttccagatcg
     2041 cctccgtaaa gctggtgcgt tatagtccga tcgctttcat ggttttcatc aatatcggta
     2101 tcaagcaggt acaaagggac cctccctacg tcgacgcgcc agactttggc aatcaggttc
     2161 cttcccggca gggcgatctg tacctgaaca taattaccat ctttggttct caccgggctt
     2221 atcgggagca ggttgaaacg ctgcggatta tactgggaaa tctgttcacc gtaaggagaa
     2281 agcaactgct tgaaataacc gtaccggtaa agcaacccga taccgatcat atgggtattt
     2341 gaatcgcttg cctgtttcag gtaatcgcct gccagtattc ccaacccgcc tgagtatatc
     2401 tgcaacgaat cgtgaaggcc gaattccatg ctgaaatatg caatacaagg tccagacttc
     2461 ctgttaccct catccatata tgcttcaaaa tcggcatata cttttttcat ctttctgata
     2521 aaggccttgt ccgattccag ttttctcaac tgctgatagg aaagggcatt gagtaccgga
     2581 ataggatttt tatcatattt cttccacagt tcggggctta tcgaataaaa cagttcgaca
     2641 gcttccgaat tccatgacca ccaaagatta tagctcagga tgttcaaagg ttccagggat
     2701 ggcgggaatg actgccggat catcaccttt ttccattcag gcaccgaatg cggttttacc
     2761 ggcaattcct ccatcacttc agcatgccgt ttatcacggt ataactcggc acgtgaagct
     2821 actttctgaa gggcgatagc gtatgccttg cgatactgcg attcaagatt ggtccacagc
     2881 gcttttttag ccagttcgat ggcatgaacg atatagtaat ttcttgtttc ttcgtccaca
     2941 tcatcaaact tcctgatgac tttcacgatc tcgtcagtca cctcaacggc gttttcgtca
     3001 ttgcggtcaa tcacgttgat gacttcattt ccgcaatttt tccgggccca taagccgaaa
     3061 cccgccagag atgtcgtcac agtgggaacg ccataggcaa ggctttcgag cggcgtataa
     3121 ccccagggct cgtaatagga aggaaaaacg gagaggtcga atgccggcaa cagatcatag
     3181 tagccgatat tgataacgcc gtcggtacca accaggtaac tgggaacgaa gataaccttc
     3241 acattgtcct gtactgaatt attgagtccg cattccgaaa tcgtattcag gatgatatca
     3301 tcgtgagggt caaacaagcc atgggtgaga tatttaccgg tattgggcga ctggaaatcg
     3361 ggagagccga tccgttcatt cagcccggcg accgggccgt aatgatgggc gggaataagg
     3421 ataaaggcaa caaccggctt ttttagctta ttttccctgt ttaggtttcc cagcgatttg
     3481 atcagtaaat caacaccttt gttgcggaat tcgtagcgcc cgctggttac cagcaagagg
     3541 ctgtcgggcg gcaggtcctg gttgagaaca gccctggcga cgtcaagcag ccgttttcgc
     3601 cccgattctc ttttcgcttc aagttcagcc ttttcaggaa tcagttcttc atcaaagcca
     3661 ttgggcgtga tcacatcggg gacgcggcca ataaaatgaa cgcattccct ggctgtgatt
     3721 tcactcactg tggtaaaggc atcacttacc aaggcggaat gcatttcgag ggaatgcttg
     3781 gccaggatgt taaaacggcg cgatatttcc ccgctgtcat actcatcaaa atggctgtat
     3841 aacggaaggt tgttgccagc aatagaccgt cccaccacag tggcatgggt tgtaaataca
     3901 cagcctgcct gcgggacgtt gttacgaagg taaagcacgc cggctcccgt catccactca
     3961 tgaaactgcg cgaccagggt atcctgcgcc gtaaggtaaa actcatagta actctgaaca
     4021 accttaccgg cagcataccc gaacaacgcc ggttctacat aatcccaatt gccggtgatg
     4081 ctgtccagtt tatacgtttc ccagaactgg gcaaatattt tatccttgga ggaaaaaaac
     4141 atggtaaaat ccaccaggat tacgataggg ttacccttga tattccaccg gccaattttg
     4201 attcgcagcc cttccgattc agccgtctta cgccaagagc gtaataaatc cggatcctcg
     4261 gtaaagtaag gattctgacg ggtttctttc cacacgtcag ggcctataag gataaaattg
     4321 tccccgtatt ctttctgaat gccgggagct ttggttgaaa caacggtgta tattcctcct
     4381 accttgttac aaacctccca gctaatttca aagatataat cgggctttgt atgatgattc
     4441 tgattcataa ttattattga tgttagcagg ttgggcaaca aaatccgtta tgactccctg
     4501 agggattatt ccgcttattc ctgattgcct gaccggaagc ggcaaaagta ctatttttaa
     4561 aattactccc gaaagcattt gtctgtgcgc tcatattcag ctacttacaa ataaactttc
     4621 gcttcctcct gatgattgcc aacagagcag cagtgagttt tactagtaaa tttccacaga
     4681 aggatggaag gatgcccggg ggccctgaga attacctggc tgctttcctg cgggtaacgg
     4741 cttttttgtc cgtaagcttg tccggtttcg tttttgcagt tctttcagga acggctgtta
     4801 cggaagttgc ttcttttttc cccgttttct ctttcaacgg ccgggcaaca tcaggctttt
     4861 gttttgcgac tgtatggcct cctgcggacc tggcttcctc atactcacgc acacggataa
     4921 ggaaatcgga aacaacattc atgtaattga taaacgcctc ataaggcgtg ctgtagggat
     4981 tgaagtattg atgcacggcc ccgtcgctga accatttggt acacatgtaa taaaaatggt
     5041 cgctgccctg caggtaattc cagtcgcgca acaatccctc atcgtcgcac tcggccatga
     5101 tatccgaaac ggaatacagc gtttcgaagg cttcatcctg gaggtcgtta ccaagccatg
     5161 ccgttacatc ccgttcttca tcagcccatg aaatggtata gggcacgtca agggaggcaa
     5221 ccggatcgag gagttcgctt atctgcgacg gcgtatgaaa attccatttc cctgacttta
     5281 aaacggttcc cggtaaggct ttcaggaatg aaaaaattcc gctctcggct ttttggtgtt
     5341 cgccgaaagt ctcataatcg agaaaaatat taactacttt ttgatcttta tcgatggcgt
     5401 taagccagtt gaggtatttt tcggttgtaa gcggccattc gctccaggtt ttctgggaga
     5461 aacggaatgc aatgtcgtcg ctgagctggt aattgcgcaa cagcaggttc agcccggggt
     5521 gattccggct gttgtataaa aaattcgggc ttttccatcc caatacctgc ctggcccctt
     5581 cggtgagcat tgtatgatag ccaagcttgt acaccatatc ggcgatatcg tttgaatata
     5641 tcagttcggt attacggaat gttacgggtt ttacgccaaa taatttcccg atggtccggg
     5701 catgttgctc aacctgacgc ctgaattccg tctcgttgtt aaaggcagcc agcgaatggg
     5761 aatacgtctc ggccagaaat tctacattac cggtctttgc cagcttcctg aaactttcga
     5821 taacctcggg agtgtatttt ttaaactgct caaggctcgt gcctgttatg ctgaaggcca
     5881 ccctgaagtc gttaccatat tccctgaaaa ggctgagcaa tatttcattg gccggaaggt
     5941 aacatttttc ggcaatgcgc ctgacgatat gtttattctg gtaatcgtca taataataat
     6001 gatcggctcc gatatcgaaa aacctgtatg tgcgaagcct gaacggctgg tgtacctgga
     6061 aatagaggca gattgatctc atgatgcaac taattttctt ggttactttt ggtaaacttg
     6121 ttcatatacg tcagcaataa ggcctgccgc cgtctcccac ttcaggctgt tgacttcttc
     6181 ggttcctagt tttcggaaca tggtagtaag cgcattgtaa tgaagcaggc tgtaaatagc
     6241 gtcggccata gcgtcgatat cccagaaatc aactttcagc acatgtttca gcacttctgc
     6301 aacgcccgat tgttttgata tcaccacagg aacattggaa cgcatggctt cgagcggcga
     6361 tatgccgaaa ggctccgata cagaaggcat cacatacacg tcgctcatgg caaacatacg
     6421 gtctacgttt tcgcctttta aaaaacccgt aaaatggaat ttggaagcga tcttcagccg
     6481 tgcggctctt cgtatcatct tttccataag gtcgccggta ccggccataa caaaacggac
     6541 attgggatcc cgatcgagaa ctttcttggc ggcttcgata aaatattccg ggcctttctg
     6601 gaaggtaatg cgccccagga atgtaaccac tttctccttc acatgtttct cacaatctgc
     6661 cagttctcct ttttcgaccg gttccaccgc gttgtgcacc gtgaaaacct tatcgggtgc
     6721 aatgccataa cgttcaatga cgatgttgcg ggtaaggttg ctgacagtga tcacacggtc
     6781 ggcttcttcc attccgtttc gttcaatatc gtaaacattc ttattgatat tttcgccgga
     6841 gcggtcgaat tcggtggcat gcatatgcac aaccagaggc ttgcccgaaa cgcgtttggc
     6901 cgccactccc gcggggtatg taagccagtc atgggcatgt ataacatcaa agtgctgttc
     6961 ggcagccaac gccgcaccta caagggcata gcgcgaaact tcggccataa ggtcatgccc
     7021 gtactttccc gaaaagctga agcgttcggc aaatacaggg tttacttttt cttccgttgt
     7081 gcggagatcc tcttccactg ccttctcaaa ctcctcagga tcaaggtaag ggatgaggtt
     7141 tgaaccgatc tccatgaagg ttatcttatt ccaatattcc atgaatgcgg gttcctgcat
     7201 cttcaccgtc acatcgctgg catttatcag ccgcaccgct tcctggcttt catccccgta
     7261 tgctttgggg accacaaaca ggacttcgac gccgtgtttc agcaggcctt tggtcattcc
     7321 gaaacaggca gtgcccaacc ctccggtgat gtgcggcgga aactcccatc caaacatcag
     7381 tactctcatc ttctatctct tataaagtta tttcttcgat ttcttttcct gtttatactg
     7441 gtcaaggagc catttcatcc gggtaacttc ggcaacactc caggcctgtg agatcgcgcc
     7501 tgacggttta tggggcggat ccccgtcgta aagctcagaa acagatccta ttccatgctc
     7561 cgtcatgact tcttccaacc cggcgtatat tttttgaatg aagcccacgc cggactcttc
     7621 atacaacttc agataccctt cggcataatg acctaagagc caagggaata ccgatccctg
     7681 gtgataagcc ctgtctcttt ccgcctgatt tcccgtgtat tccggtttat aggcagggtt
     7741 gatcggcgag agggtacgga tgccccgcgg ggtcagcaat tcgctggtta cacggtcgat
     7801 aacgctttta cattgctctt cctgcagtgg ggaatagggc agcgatgccg caaatagctg
     7861 gttcgggcgg acggctttat cgcgataatc tccataaaca taatcggcca gataattttt
     7921 gtcggctatc cagaaagttc taacaaattc gtcgggaatt ttttctgcga tttcttccca
     7981 gtctttgaca aactgagaat ccccgttttt acgagccagt tccagcgcaa acataatggc
     8041 gttgtaccac aaggcattga tttcgaccgg gaggccgatg cgcggggtta caggctgacc
     8101 ttcgacgatt gcgtccatcc aggttacggc agtgccttcc tgacctgcat aaagcaatcc
     8161 gctgtcaagc atatggatat tgaacagggc tccctgcctg aaaccgtaaa gtatggtttt
     8221 cataggcagt tgataatatt tccatacatt tttatcgcct gacagaagca tatattgctg
     8281 gagcgtccag aagaaccata acggagcatc aacggaattg taaacggcgg aaatcccgct
     8341 tcccatatga gggaataagg ggcctttcat ctcggcaacc aaactgtcaa taaccgcttt
     8401 aaaggtcctg taatctttct ggagaagggt aagccccggc aacgcaataa aggtatcgcg
     8461 cgacatacgg ccgaaccagg gaaatccggc gagtatttct gtatgttttc ccctgtgcag
     8521 tataaactga cgggccgaat tgagaagaca atgctcaaaa ctgtcgcggt gaacatgttc
     8581 actgacctcg tcttcgtaaa tgcggatgat agattccgga tttgcttctt cgaggcttac
     8641 agcaaaataa atgctttcac ctttgtctat cggaagttca aaagttccgg gagaaagaag
     8701 gtcctccagg taatcgtagc cccttgcttt ttcgcggata tactcaaaat tgaagtacca
     8761 gtcaggatga tgggtataaa caggtttctt tgaaaactgc atataaaggt ttgaaaagcc
     8821 cggatacagg cgcacataaa taccattgag cgcttcatga taatccttgt cagcgtaacc
     8881 gtttgctttg ctcacggcat gtatgttccg gaatgcgagg aaaggcctga atctcatcgt
     8941 cgtcggcgaa tgagcgtcga ccagggtata ttttattata caccggtcat cacccgcgat
     9001 gaagatcatt tccttggtca ggatgacgcc gcccacacga taggtaacag ttggcaaagg
     9061 atcagaaaca aaatcggtga tatatttatg gcctttggga ctgtaaacat ttcccatata
     9121 ccggtgcaat gcaaggttaa attcggcatt ctgctggatc acggtctcat ccagggatga
     9181 gagcagcaca tgcatgcccc cgtcgatctg gggctgcggg gctaccagca agccatggta
     9241 tttgcgcgta ttacagccaa tgatagttga acaggcataa gaacctgccc ggttcgatct
     9301 gagcagttcc ctggtcaggg aaaactcgag gttcaccagt tgatcttttg taaattgaat
     9361 gtaactcatc tcaaacctga attttgcact ggatataaaa atgtcgatcc cctttccgga
     9421 acaaacatat cattccgata aaatgttcct ctgaaacatg ccgtcacagg aaaattcaac
     9481 atctgtattc aactctaaaa ataataaaat tattaaggga ttcgcccttc tgataataat
     9541 aaattatcgg tctgatcgta ttatactttc gttaatctta cttttgcacg aaatatcgga
     9601 tgatgacttt tcctgtaata ccgaagaaga tctcaacctg gtccctgttt atcctggctg
     9661 gttttgccgt gatgtcaggc tgccggaaag aagagtttaa taccgataaa aactacatgc
     9721 tgaaattcag cagcgacacg gtgatcttcg atacaatatt tacttcggtc ggaacaacta
     9781 cccacgttct gatggtttac aattcgggca gcctcccggt aaaaatatct tccatcaggc
     9841 tcgccggcgg cagcgcatcc cctttccgga tcaatatcga cggtgaagcc gtatcacaga
     9901 agtcggatgt tgaaattgcc gccaaagaca gcctgtatat cttcgttaag gttaccattg
     9961 accctaacga tgccgacggt ccgttcatca aagccgattc cattcttttc gagacaaatg
    10021 ataatgtcca ggatgttaaa ctcattgcct gggggcaaaa tgcatatttc tatcatgaca
    10081 cggtcctcac cgggaacatc atcctgaaaa atgacaagcc ccatgtgatt tacggggatc
    10141 ttattgtgga ttcgctaagt tcccttacca ttgaagaagg cgctcaatta tgcttccaca
    10201 ggaacagcgg gctgatcgtt aaaaacaccg gcaaactgaa ggtgaacggc accctggcag
    10261 acccggtcgt tttcaggagc gacacatacg taaacttcga cccgttacac gatacggttc
    10321 ccggtatgtg gtcaggaatc cggctgcaaa gcggcagcgg ccggcatgag ttgaactttg
    10381 ctgaaatacg gaacgccgaa gcaggcattg tcatcgatag cgctgatgcc tcccttgccc
    10441 ctgttcttac tttgtacaat tgcaaaatcc ggaataccgt cagttatggc atctctgccg
    10501 ttgcctcttc agttttagct gccaactgcg aattttcaaa ttgcggaggt ttcctgctgg
    10561 ccattcagca aggcggaaat tatgatttca ggcattgtac gtttgccaat tactggcaat
    10621 ggctgcgaaa aacgccatcc atttacctga gcaatatcta ttacggactg ctgggagaga
    10681 agcacttaca ggcgctcgaa aaagcctact tcggaaattg cgtcatatat ggcaattaca
    10741 gggttgacac cacacgcacc gaattgaccg tatcggaaga taaaagcact cccttcagtt
    10801 tcaccttcga tcattgcatc attcccagcg gcctgaacaa gaattatccc gactcgtttc
    10861 acagttgcct ggtcagggaa gatccgctgt tcctggatac cgaccactgg aattaccagc
    10921 ttgacaccct ttctcctgcc aaagatatcg gcttacgcag tatcattgac ttttcatcgt
    10981 tgaacctcac ttccgacctg aacggaaatt cccgcgtgag cgacaccggc ccagatcttg
    11041 gcgcgtacga aaggatagaa aaccggtaaa ccatagaccc tggttcccgg ctcctcatat
    11101 tcccggcaat cgccggccgg cattttcccc gattttactg tgttcccagc ataatccgtg
    11161 aagagatcaa catgccctga agctgttaaa cagcaggtct ccccatgagc ttcagcatgg
    11221 cggttcctat atcggcagga gaatccacta cggtaatccc acactcacgc ataatggctt
    11281 ttttagcctg cgccgtatcg gcttttcctc caacgatagc gccggcatgg cccatggtcc
    11341 ggccttttgg cgccgtagcc ccggcaatga aacttacaac cggtttagtc ccgtgatcgc
    11401 ggatgtaaaa ggccgcctca gcttccatat tcccgcctat ttcgccgatg atcacgattc
    11461 ctttggtttc gtcatcagcc atgaacattt cagcagcatc cctgatgctg gtgccgatga
    11521 tcgggtcacc gccgataccg atgcccgtgg tttgaccaag tccggctttg gtaagctgat
    11581 ccacagcttc atacgcaagg gtccccgaac gggagatcac gccgacgatg cctttcttat
    11641 gaatataacc cggcatgatg cctactttgg tctctcctgc agtgatgatt cccgggcaat
    11701 tcgggcctat cagcctgcaa tcttttccac ggaggtattc tttgaccatc agcatatcct
    11761 tgacagggat tccctcggta atacagacaa taacgctaat ccctccttcg gctgcttcca
    11821 tgatggcatc agccgcaaaa gcgggcggga caaagattat tgagacatcg gcgtttgttg
    11881 ctttcacggc ttcccttacc gtattgaata tcggaaggtt aagatgcgtc tgtcctcctt
    11941 tgccgggagt tacgccacca acaatggtag tgccgtattc catcatctga gtggcgtgaa
    12001 atgtcccttc gctgcctgtg gatccctgca cgatgacgcg cgaatctttg tttaccagga
    12061 tgctcatcgg ctttcatgtt tgatcttata tcaaagatat tgaatttttc atgcttttcg
    12121 actttaacac cccgccaaaa ctctattcat tgaatttgtt actagtgcct aaagtgccta
    12181 aagtgcctga agttgtgagt tgtgagtttt gagttctgag ttctgagttc tgagtttcct
    12241 ttgacctttg tcctgccgtc ttacccacac ccgccccagg caggtaaact tttggctgtt
    12301 gggtttcgtg attcattgtt cgcaattcgt ggaattcgtg gttgcattcg tggaattcgt
    12361 ggttcattat tcattgttcg tgatcgacat tcgtgaaatt cgtggttcgt ggcaccatcc
    12421 tccgtactcc gttttccgta ctccgttttc cgttctccct gctccctgcc ccttgcttcc
    12481 ttttccaccg cattttgttt aaaagtatta tcggcctctg ctcatttttc tccggaaaat
    12541 atgacttttg tcacatggtt ccctgcagca gttttaacaa aacattatca ggaaggcgga
    12601 ttatcagcat atttctgctt tttgctgtta taatttcatg gtcatcctgt tcacggagaa
    12661 aaaaagaagt tgcccctgca gatgtccgta attgcataga tctcagattc ccgtcaaaaa
    12721 aagaagaagc ctcgattcgc cgggaaatca atgccgacct gaaatccagg cgattagatt
    12781 ccctgttccg tgaaaaagca aggctccggg ggttcaacgg ggcagttctc attgcccagc
    12841 ggggagtcat aatttataaa aacgctttcg ggtataccgg tcccgaccgc aaaacgcccc
    12901 tcaccaccga ccatattttt cagttggctt cagtatcgaa aacattcaca gccattgcaa
    12961 cgctgatgct tgccgagcgc aaggcactgt cgctcgatga ttccgttcag aaatttttca
    13021 gagactttcc ttatcacggc atccggataa aagacctgtt gagccatcgc agcggacttc
    13081 cgaattatat ttatgccttc gatgaatggc gtaacacggt gacggttccg ccgacaaaca
    13141 gcaccatcat gagatggttt gccgtatctt ctccccctcc ggtaccatac aacctgccgg
    13201 atgtggcatt cagctacaat aataccaatt acattattct ggcaagcatc atcgcaaaag
    13261 tttccgggaa aacctatgct gaatttttaa aaacagaaat ttttgagccg ttgggaatgg
    13321 tccacacttt tgttgatacg ctttgccctg tttcactcaa agcattgaag actttcggcc
    13381 atgactataa caggcgccgc gaccgcgact ttttcgacgg cgtttacggc gataaaggga
    13441 tatattctac cgttgatgat atgatgcgat ggtatatggc gctaagttca cactgccttc
    13501 tcagccgtca cctccttaag gaggctttca cgccccgcag cctggaacac cggagccggc
    13561 acaattatgg ctacggattc cgcctgataa ccgacccagg aaacatgaac aaagtggaat
    13621 atgtctatca tggcggatgg tgggcaggct attcaagcat gatattgttt gatcttcgcg
    13681 acgactatgt cgtcattgta ctctcgaaca ggaaaaacac ttcggtttat gaaaacaaaa
    13741 acgtcatgga catcctggat gacaaaccga ctgacgaaaa tgaggaagaa atcgactcca
    13801 tctaacgcac acacgcataa cattttaacg atgaaagtgt ccgggaatcc ataatttaat
    13861 tatcccgatt tgaaaaattg aaaaatttca ttatctttgt taatacaaac gtggcaaaat
    13921 gaatatcagc agcacttttt cagggatgga cgacacgata tgcgccttat caactcctcc
    13981 gggaatgggc gccattgcaa tcatcaggct ttcgggtaat ggtgctttgg atgttctgtc
    14041 gtcggtattc aggcccttaa aaaaaagcat taaggtgccc gagatacaaa gtcatacaat
    14101 aacgttgggg acattcgggg ccggtaatga aaccctggac gaagtacttg tttcggtatt
    14161 tcgcgctccc cattcctata ccggtgagga tgtcgtggaa atctcgtgtc atggatcggt
    14221 atttatccag caaaaaatcc ttgaaacgct tacctcttcg ggagcccgta tggcccgcgc
    14281 aggagaattc accctgaggg ctttcctgaa tcataaattc gacctcaccc aggcagaagc
    14341 cgtagccgac ctgatcgctt ccaatgccaa atcatcgcac gaccttgccc tttcgcagat
    14401 gcgcggcggt tttgcgacga aaatcaaaaa cctgcggcag cgactggttg acctggcctc
    14461 gctcctggaa ctggaacttg atttcagcga agatgatgtt gaattcgcag accggagaca
    14521 actgctcgat accatcgaaa gtctggacga tgagctcagc cgccttgccg gttcattcaa
    14581 actgggcaac gtaatcaaac aaggcatccc ggtagccatt gtcgggaagc caaacgtcgg
    14641 caaatccacc ctgctcaacg cgcttctgaa cgaagagcgt gccatcgtca gcgaaattcc
    14701 gggtacaacg cgcgatacca tcgaagacac ccttaccatc aaaggtgtat cgttccgttt
    14761 catcgacacg gcgggcctgc gtctgcattc aggggatatc atcgaaaacc tgggcatagg
    14821 gcgaacctat gaaaccatgc aggacgctac ggtgatcctt tatgtgttcg acatgagcca
    14881 gataaccgca gatgagatcg acgaactgct ggaagaattc agggaacaca ttgatgaccc
    14941 ggaaaaacat ttcatcatgg tcggaaataa aacagacctg atggtggaag tcccaccgca
    15001 tgttgccagg cttttcgata ttgaaacagt attcatatcc gccaagcgca aagaaaacat
    15061 taacctggta attgaaagtc ttctgcgggc cgttaacatc aaccagcttg ccgattctac
    15121 catcgtaagc aacgcgcgtc accatgaagc catcaccaaa accctttccg ccctggaaag
    15181 cgcccggcaa gccattgaaa acaatacaac ggccgacctg gtagccatcg acatccattc
    15241 agccctgtac catctcggga ccattaccgg cgaagtaact acaaacgaga tacttggaaa
    15301 tattttcggg aagttttgta tcggaaagta aatcattccc gtacggtcag gttactgaaa
    15361 ctccttttta agagtttcaa cccaaagccg gattcgctga tccgtttttt ccggctggct
    15421 ttcaacatcc aggaccaggc ctaagaactg cccgtcgaca acagccctgg agcgttcaaa
    15481 agaataacct tctacagatg tttgccccac aagcctggct cctgcaactt caaatgcatc
    15541 agccaaaatc cggattccat ccacgaagtt ttccggatag tctgcctggt ttccgatccc
    15601 gaaaatagcc acttttctgc cctttaagtt cagggtattc aattcaggga ttaactcgtc
    15661 ccaatatgcc ggcagttcac cgtcgaacca ggtggaggcg cctgcgataa tgttatcgaa
    15721 gcgttcaaaa tccttctgcc aggcggtctc aacaggtaca acctctatcc tgaaatcgcc
    15781 gaaagcatcc tgaatttttt tcgctgcttc ggatgttata acggcgtttt tactgtaaaa
    15841 tatcccggtc tttttcatct tataaaagtc tttaatacaa taacaattcc ctggctgcct
    15901 tgtaaatacg ctcctcatcg ggaaggatgg ctttctccag atcatggtga aacccgaccg
    15961 gagtgaaaag agaaccgacc cgctgaacag gggcatccag gtgccggaac atctccgaac
    16021 cgatcatagc ggcaatttcg gccccgaatc cggaaaagac tttgtcttca tgtacaatga
    16081 gcaccttgtt tgtcttttta acggattgaa agatcgtttc tttgtctaag gggatcagag
    16141 aacggatgtc tatgacttca acactccggt tttcttccgc cttcaaacgc ctggcgactt
    16201 tcaggctaag atgcgtggta ttgccgtagg taatgatgct gagttcatct cccggaagcc
    16261 tgatcctggc tttgccaaag ggaacttcaa aatcgtcagg aacaaaagtt gccgcatctg
    16321 ctgcattata aagagcctta ggctccagga acagggtgaa tccttttgaa cggatgctgg
    16381 tacgtaaaag tccggcggca tcgtcggcaa aggaaggata tacgatcctg gcgccaggaa
    16441 gattgcttaa cgcgccttca atggtttgcg aatgatacaa ccctcccccg atatacccac
    16501 ccgatgccag gcgcagagta atatttggcg tgaatttccc gttactccgc cagtactcat
    16561 gggtacattc aacatactgc tcgacggccg gccagaaata gtcggcaaat tcagcccctt
    16621 caataactat gtgaatttta ggatcaaagc ggctcatacc attggccgtg cctacaatgt
    16681 aatcttccgc gataggtgca ttaaacacac gcttctcgcc aaattcctgc tgcatgccct
    16741 ttgtcacgtt gaacacgccg cctttgttct tgtttgcaac atcctgtccc caaaggaagg
    16801 tgtcagggtt atgacgaaat tcagccttca gcgtttcgtt tatggcggta accaagttct
    16861 tcttctcacc ttcaccggca tgcgttccgt cagtgtactt ctgaggccgg taaggttcag
    16921 gaagcacata atccaaaacg gtggaagggt cagggtccgg agcggcaatt gcttttcggc
    16981 cggcaatact cagttgtact ttagcgtcat cttctatctg tttgatttct tcttccgaaa
    17041 agcggttgta acggagcaac atccggcgga atttctgcag gggatctgcc gcttttacat
    17101 aggacaattc attatcgtct ctatataagc cctgtttatc agagttggaa tgagggccga
    17161 tacggacgca attcgcctgg acgatcacgg ggttaccggt attgaaggca tattcgcgtg
    17221 ctgccgtcat cgcattcatc gaatcgaaaa cgtcttttcc attacaatgg atgattttaa
    17281 gatttttaaa tcccgagaaa ttgtccgcaa ctttccgatt ggcagtttgg tcttttttgg
    17341 gaaccgagat accgtatccg ttatcctgaa tcacaaatat caccggaagc cgttcagtac
    17401 tggcgccgtt aacagcttca taaacaaagc cttctgacat ggttgcctcg ccgtgagatg
    17461 taatcacaac acctttgtgt ccgtaatact gcatggcccg ggccacacct acggcatgca
    17521 aatcctgggt gccggtagcg gaagaaatat tttcgatatg ccattccgtt tttgcaaaat
    17581 gatttgacat gtgtcgtccc ccgctggtcg gatcagtgac tttggaaagg ccgttcaata
    17641 ttatttcctc cgcagtcagt ccggccgaca gggccgtgag caagtcccgg taataaggaa
    17701 ataaaaaatc ttcgcctctt gtaaaaacct gtccaatggc cagttgtatg ccgtcgtgtc
    17761 ctgcataagg ggcgtggaaa gaccacccga gcgattgaag caggtaaact ggagcctttt
    17821 catctatagc ccggccgagc gccatcagga aataccattt tttcagggtt tctttatctg
    17881 tcgacagtat atcaaatttt ttcatgttca tgatcattac ttacaccgca atcttcagct
    17941 atttgtgtcc caattttcga gaaaatcggc aatccgccgc aaaaaggctc cgccgaccgc
    18001 cccgtttatg attcgatgat cataggagag agacagatac atttgatgcc tgatggcgat
    18061 tgcatcccct tccggagttt cgataacggc aggttttttc ccgatgtagc ccacacccag
    18121 gattgccacc tcaggctggt tgataatggg cgtgccgatt atattcctga atgtcccgaa
    18181 attggtgatc gtaaacgtgc ctccctggac atcatcgagc gttaatttgt tggtgcgcgc
    18241 tttttttgca agactatcga tcgccaaggc aagtccgctg atgtttaatt tgtcagcatc
    18301 atgaacaacg ggcactacca ggttaccgtc atcaagcgct acggcgacgc ccacatttat
    18361 atgcttcttc aggatgaact tatagtcgct gaccgatgca tttaatttgg gaaactcttt
    18421 caaagccatg gctgttgcgt tgactatcgc cggcatgaaa gtaagtgcga cgccttcctg
    18481 cttcatgaaa gaatccttgc ggctgtctcg ccacttcacc agcctggtaa catcggcttc
    18541 aaccaccgtc gttacgtggg ccgaaacatt cttcgactgt atcatccggt cggcaataat
    18601 gcgccccaca ggatccatgt ttacggtttc cgagtagtca tccgcagatt ccgttactaa
    18661 aacaggggcc ggcaccggtc gattttgtat aggcggagcg gaaagcgctg cttcaatcgg
    18721 gagtttatcc ttggaggcga aaggcatttt ccctttcttc ttctgttcaa tatactcttt
    18781 aatatcctta ttgctaaggc gtcccaaata accggttccc ggtacctggc tcaactcttc
    18841 tgcaccgata ccggcttctt tagccaattc catgacaacc ggtgaatacc agcggtcatc
    18901 tttaattccc ttttctgttt tcacagccgg gcctgttaaa ggctcctgaa aggatgcatc
    18961 agacccggga ctttccattg ttgaatccaa agatccagcc ttatccgaag cttcagcctc
    19021 atcaatccca tccggttgta tcacagccac tacgtttccg accggaacgg tatcgccttc
    19081 gctgtaaagg atttttataa ggattcccga aaccggagat ggcacttcgg catttacttt
    19141 agcggttgtc acttcaaaga ttacatcatc ttcgtttacg tggtcgcctg cttttacgag
    19201 ccaactgaca atcgttcctt cggtgatgct ctctcccagt ttgggcattt ttatatcaaa
    19261 tgttggcata taatgtcttt tttgtactga ttattaacaa tcaacaggca ttaattgttc
    19321 cgattttctg attaaatgaa agaccgtcct gcaggttcca tgccgacgtg gcatatttga
    19381 cccgtctcag attctcaata gcccgtatgt cctccctgct gaaccggtat aggctgttgc
    19441 ttttattgta tctcaggaaa tattggaaaa tatggttttt aaaatcactc agagtcatcg
    19501 gcatcggtaa gtaatcccgg atattggtta cacggcttct cacagacttc acatatcggt
    19561 ttcgttcagt tggtcccata ttttcaaaat cagcatatcg gccttcgagt gctgatgcca
    19621 tattgataag atcagtagaa aaaagcaggg tcgcatgata cagggtctta tcgccacgca
    19681 cgaactgcgc gctccccgat attttcaggc cgtcaacata cagcgcccgg cgtgcatcag
    19741 tttgtgcatg aatcccaagc gttgaaagca gatgctgcat ttgttttgca tacttgtcaa
    19801 aatcggcact gttgccagtc tccataaagc taagattcaa attgccgtaa tcctgataca
    19861 ccgctccgcc tcctgaattt ctgcgcacta ttttaatctg tttttcacgc acgaaatccg
    19921 tgtttatttc cgcatcaacg cactggtgcc ttcctatgat cacgctgggt tcattctgat
    19981 acagcatgaa aaccggagtt gagaaattcc tgatgagata ttcttctgcc gccatgttga
    20041 aatatgcatc cgtctggtca ttcacgatac agagcatttt cggtatttca ttttctgaaa
    20101 tatcagccat gtcacctgcc ggctgtctca acttagtctc attgtcgttt ctcatggcat
    20161 actttattat agccggcacc tgacatggta ctgcccatgt aaataaacac atatcaggag
    20221 attaagtttt gttgcatctg aaaaagggtt cgggttatct ttgtatacaa tttcccaggt
    20281 tccttccttc ggctcattta cagttatttt cctgggcttc cctccactct tttaatcttc
    20341 cggctacttt gtcattcaaa ttcattcaaa cggaggaatt ttatgaaaca aataaaggag
    20401 tttatcacaa ttattatcct gctgtcgact ctattaccgg cgttttcgca aagcggatat
    20461 tcggtggagt ataaccacaa atacatctgg aaagatgaag gaattgatat ggaaataacg
    20521 gtgaagggag aagtgccctt tcacacagga acaaacttcg cggtgatacc gaaatgccag
    20581 accggcagcc atgacaggct ggattcggta aaggtagttt cgatactgga aggggaaggc
    20641 gaagtcatgg ccaaaggcaa atcaatactt ggagataaga gcgatcccga aaacctggtt
    20701 gtaatggagt acacccttcc ggtaaaaatc aaaatcaccg ggaagacctg gtacaaggac
    20761 gttacctgcg ccgaaatgtg gaacctggcc ctggaagagg actggaccgg taaagtggaa
    20821 tggaactatc aggcaaagtc ccctgaagca gcagccgtag cgcaggctct gagtcctgcc
    20881 acattcggtc agatgaaccg gctggaagac cacacccttg aattccagag atatcctgtg
    20941 ttttccgaca aaccgctggt gcttatcgaa gacgcattcc ccgatatggg cttccccatg
    21001 aaaggcaggt tgttgtttcg cgtaaatatt cccggtatcc accgcatcgg gatagatgaa
    21061 gagtatgatt gggacgaaag tatccagccg aataaagagt acaaattctc cgatctgcat
    21121 tgggggccgc ctctcgacca gatcaactgg aatgttacag atgtggataa aatcaggaat
    21181 tgattgccgg taattggaag aatcaggtat tactttcccg tactgctccg attccggagg
    21241 ataccgccgg ataatttcaa aggtttattt gtctgtaaac atcgcgggga cgggattggt
    21301 caatgcccgg ctttgattgc tcacgcgcat cagttcagcc attctgtttg ttcattttta
    21361 tccgtcagac aaaattgaca atgtcaacca aaataaaata cagaccagcg taagaatttt
    21421 tcaagacatt ttatatcttt acattaacat taatatccgg acttcaatta tctgtggggg
    21481 aatcaccgat aattaatctc gttatctcat catttatttg acttgtctgt ccctattctt
    21541 ttcatgttta tagagaaata atcagtgaga attattcaga taacagttta atattaaatc
    21601 acatacactc atgtataccg tcgatatatt gctgcccgca tacaacggtg aacaattttt
    21661 aaaagagcag attgaatcgg tattgagcca atcataccct gaaatcaggc tctacatccg
    21721 ggatgacgga agtactgacg gaaccgggga acttttgaga gcatttgccg cgtccgacaa
    21781 aagggttact cttgttgaag aaccggcggg caaccttggc ctgataaaaa gcattgaatg
    21841 ccttctttct cattccgaat ccaggtatat catgttttgc gaccaggatg atgtctggtg
    21901 cccgaataaa gtcatggaga tgctgaatgc catgctgaaa caggaactga agagtgagcc
    21961 gggagttcca atcatggttc attcagattg ttttatcacg gatcaccggc tgaatatccg
    22021 gggaaggttg cttgggaatc tgccggcacg ttccggcctt aggcatgcct tatttaccta
    22081 tatggtgcaa ggcgcatcaa tgatgatcaa tctcgcatta aaagatgtga ttcttccttt
    22141 taacgggtac gtttatttac acgacaggta tattcatctt gtttctgaaa tcaccgggaa
    22201 aagggtttat cttgattatc ccctgatgta ttcccgtcag cataattaca attttctgaa
    22261 agaaagatct ttcctccata agatttcaga ctatatattc cggccctatt atacctattt
    22321 tacacaatat gaccggaatt taattcaatc tttatccgaa aggtttcctg gcaataaatt
    22381 actcaacatc tatcttgata tcacgtcgga taaaatttct aaattcagga aaatcaagct
    22441 attggcatcc aaccatatct cgatgaaacc caaatatctg attttgttcc tgattaaagg
    22501 ataaaaatca tgatcccgcg atgcggaatg ctgaaaatta aaaagcggcc tcagattttc
    22561 aatcattaca aggctttgga atccggggct ttttatatcc ggataagagc atattcttca
    22621 taattgtgaa ccggacaccc cggcaaaagt gcggatcgtc ataacaggaa taacggcaac
    22681 cgctaccaaa attccatgcg gtgattcggc agcattgggt gagtcatttc gtccgtctcg
    22741 ttatccctga cgtgattccg cgttgccatt cagcccgtcg ttccccttta aaaccttccg
    22801 ggacgaaaaa tccggagaaa taatacaaaa cattcaaaat ttttacttac ctttatattt
    22861 ctatctgaac actttgtagg tatcggcaaa ctctgattgt ctgatttatt aaactgaggg
    22921 ccatgagcaa gcacctttac ctgaccacat cccctgaatc attgatcgct tcaatgcttc
    22981 cgcccgttga attcgggact tacctctcca ggggcacaaa gaacaggaac aaaggagagg
    23041 caatttactt tgagatagac ctgttccagg ttgaaaaatt gattgatctg gatagcctga
    23101 atcgccgatg tgtagccaag cccgacggca gcccgaaatg ttccgtttat ctttccattt
    23161 acagggtact cgaaaaattg cccttgtcag cctttaaaaa cctgtacctg acaaccgaaa
    23221 acgggcatac actggaactg aaaaaatccc cctataactc cggtaaggaa gttagaggca
    23281 agctgcacct atatcaggaa ctttgccctg ttacgcctat ggttgccagc atactggctc
    23341 cttctgcttt cctgaaacgc cttaccgacg gatccagccc cattgtcctt ccgaaacttc
    23401 tctttgttga cctcaagtta ggagaacttg ccacaaatcc gctcagaggt tccgccgaac
    23461 aactccccta tcccaatgtt ggccaccttc gcgactgcct cgaaatattg agagaagaag
    23521 aaggcaaaga aatgaaaacc gtcctgcgct ttttcttcgg actgctcctc ttcagaacca
    23581 ttgaaagcgg ctttttcatc ggggcaaaag acgagatgat ttactatcca tacccgagca
    23641 tatcggagct ggaactgatc aattatgact ttttcaggtc gatttagcgc tggcctgccg
    23701 atccgataac cggagcagac ataaagccat gacattcccg gaactgatcc cgggccggtc
    23761 agcaaaagac agaatcattt ttttaactac taatgatccg gattgtgcca gtttgggcgc
    23821 tacctgtcat tttttagatc catttcgcac ttcggttctt ccggcttatt catacctcag
    23881 cgcctccacc ggattacgcg atgctgcttt ccaggtttgc cggcttacgg taaacagtat
    23941 tatgatcaga atacaaattc cggtaagggc gaaaacccac caactgatcg gggttttata
    24001 ggcgaagtct ctcagccaga actgaactga taaccatgat accggtgttg ctataattac
    24061 tgagacaaaa gcccacatga tgaaatcctt attcagcaac aggagtacat cgtaagtacg
    24121 tgctccgttt actttacgta tcccgatttc tttaatcctg cggtttacag taaacagcac
    24181 catgcctgtg atgccgagca gcgaaatgat aagggccgcg agcccgagtc ccagggtgat
    24241 attcctgagc cgcgtttcgg ctttgtactt atcctgaagc atatcctcga aaaacatata
    24301 tgacatttcc ctcccgtcgc ggtacttttt atacactttt cggacgtatt caatggttgc
    24361 ctgacggtta tgcggtgaga tgctaacatt catggtcatg atattctcag ggtcgttcat
    24421 gatcataacc ggttcaatcg gataatttaa aggcctgaaa tgaaagtctt taacaactcc
    24481 gacgattagt ttttcgttta tccgttttcc aacgatatcg ggaataccga gagttttctc
    24541 agcagtttcg ttgatgacaa ccggcattga aacctcgtca tcggcgtcat cattccccgc
    24601 tttgcttttc tttttccgtg cggcccagta atcagcgtac tttgttggaa taaactctcc
    24661 tttcaggatt ttcagatcat atgtttttgc gaaatcctga tcagccatga tcattgacat
    24721 tcgcacacta tcagattttc ccaatgaatt caaaggcaat gatacctgcc acgaaaaatc
    24781 gacaggagca taggcgcttg ccgaaacagc cttaatatca gtgtttttca gcagttcatc
    24841 cttgaatgta tcactatcgt accataaccc tgtgggaatc acaacgatat tttctttctg
    24901 aaggcccaga tcttttgtcc ggacatattc caactgtttt atcagaaatg ccgttacaat
    24961 gaggaagaat gcagcaaaac ttacctggac tatcaccagc gtccttatta atcctgcttt
    25021 cgaaccggtg tcagcgcctc ctttaatgat ttttaccgga ctgagcgccg acaggcaggt
    25081 tgatgggtac aatcctgcaa gcaagcttaa aagagccgtt agcgctatca agctgataat
    25141 taatctgaaa gataaaatta tccggatcga cagccctgtc atgtcattga ctgcaggcag
    25201 caacaaacag agcaaaatca ttgcaagcgc tgctgcggaa aaggtctgaa aaaaggtctc
    25261 cttcaaaaat tgaaacaaaa tctgcatgcg gctggcgccg ttcacttttc tgatgcctat
    25321 ttctttcgac ctttcggaag aagaggtaat cgaaaacatt gaaaaattaa aggccgccat
    25381 gaagaggata aatcctccca atagcgagag aacccataaa tatttgatat tgcccgggtt
    25441 accaaccagc atatcatcaa aatccgattt caggtgaatg tcggctaaag gctggaataa
    25501 aagcttgtct ttaatgggaa ggtatttctg tatctggttt gctatcgcat gtttaaaatc
    25561 atcgttaatg actgcgtttt ttgcaagttt gatgtagacc cttgtccagt aactgtcggc
    25621 ccattggttg ttataattat cgccggtttc aggtatcaga taatcgaact gcaggctgct
    25681 gttgtcgggg acgtcaacca cagcgcctac tgtgtaaatt ttcttactgt atttttcgga
    25741 aattattgtt ttacctattg ccggtgtttt accaaataat ttttccttga ttttttttga
    25801 tataacgatc tgatcaggaa tcagatacaa ggcatcggga ttgccttcaa gaaattcaaa
    25861 tcccctgaat accttgaaaa aatcctggtt aacgcttgcc tgccgagctt cgataaaaga
    25921 tgaactgttt ccggttttca gcggcgagtc ctcggatgaa tatgaaatgt aagccgcaaa
    25981 ttcaatctgc ggataatctc tttttaatgt acctgccaac gggcgatagc ttgcagccga
    26041 tttcctgacc gcatcatttt gctttgacaa agtcaacacc ctgtatatat tctgaaaatc
    26101 aggatgaaac ttgtcgtact gcaactcctg aaatacccat aaataaatca gcaatcctgc
    26161 ggttattcca accgtgaaac cggcaaatga cagcagggta tatatgagtt tcggacttcg
    26221 tctgaaatga aatatagttt tgtttatcag catattatgt tatttataga tgaaaataac
    26281 catgtcattt tcctgtaatg tttccatcgg cgcagcctgt ccgggtctga aataacgcat
    26341 cagcctctta cccgaaacgg cagcaaaagg tttgttatca gccgtaactc ctgtcggcca
    26401 gaaaaacgga cagccgttca aatcgttttc gatatttttt tcaggagctt ttggccatga
    26461 aaaaggttgc tccggattta tttcgggctt ttcacatgaa taaacagcga tgacctcatg
    26521 ttttgattta tcaaaaatta tcctgccata tttcaaggta ccatgctttg cagtgttcgg
    26581 acaggaataa tccctcgtat atgtgatgaa tatgtattta tcgctttcaa taagatcatt
    26641 tagcagcagt ttatctttca gattgaattg cggatccatg ccttcagtag aattgttaac
    26701 gccgcgatcg ccgaaatgca ggacaaattt tgctaaaagc ctattgggcg gcaggagttc
    26761 aaagattgta tcgttaaaag ccggttttat atgaaattga ttatcaaccg gatacatata
    26821 attttcgtcc ggagaacgat ataccctgcc ttttaaatgt ttcaccggat cgtaatcatt
    26881 aaatatgcag acagtatctc catttttggt caacagggtc agcaaaggag catttcctgc
    26941 cggcgatttt gctgaactgt aagtggctaa ttgattgccg ggtaatatgc aatagaggtt
    27001 tggattaatt ccattgccgg aaggtttccc aaaatcgatg actgtttttc caaaaccttc
    27061 aaaattgcga tggttttcaa caccggtccg gttgtcgctt attgaaggag cattatcagc
    27121 atcatcaacc attttaagct cacactgatc cccgggttga ttttcgtact ggtaataaaa
    27181 tttaccatca acaagacgcg cactgacggc acctgaaaat tgttgtttgt attccctggc
    27241 atccactgca acagaacctt ctccgttatc aatgtaaggc atattgttat cgcaaatatc
    27301 ttttaccagt cgtcctctca tatcatactg cctgattcct gtgaaacgcg aaaactgata
    27361 gatgtgccgg ttgctgatga tataattctt tgaatagtgg ctgagtggcg attttgtttc
    27421 atcggctgga attctgacaa cctgaatttg ttgaaaaagc cgggaagctt ttatcttagc
    27481 cgcatgatcc tgaacagcag caatatcaat aacaacaggc ggatttgacg gatctgcgga
    27541 ccggatttcc ttatatctga tccccggttt ggcgttggag aacgaacccg ttgcttggcc
    27601 gttctgccaa acaccggatt tttgctcatc agccagttct ttgacaagac tttgttcatc
    27661 cattttcaac ctgttccggt tgcaggaagt ctcgccaaca agcaacaata acagcgcaat
    27721 caaaaggatc tcgaacttca tatattattt ggtgtttgtg acaacgattt ttctcattcg
    27781 tagcgtaacg cttcaacagg gtttctggtt gcagcccgcc agctttgcca gcttacggtt
    27841 aataaagcaa ttaccagcgc caggaaaccg gctaatgcaa aaatccacca gctaagcggg
    27901 gttttgtagg caaagttttc gagccatttg gtcatggcaa aataagttac cgggacagct
    27961 gctgcaaatg ctatactcat ccatttgatg tatgatttgt tgagcatcag caacacatcc
    28021 gatattttcg caccatttac tttacgtatg ccgatttctt tggtcctttg ctgtatgtta
    28081 aaggaggcaa ccgcaaatac ccccaacgcc gacagaatga cagatatgat tgtaaacaac
    28141 gacaatacgc ggctttggtt gtattcaacg gaataaatgc tccggtatga gtcggagata
    28201 aaggtatatt caagcggcaa acccgggaac attttcttcc acacggaatt aaccgtcgcc
    28261 agcgcttctt tttggtgtgc agaatcaaaa cttatgataa aattggaact gaatgattta
    28321 ttggcaaata taacca
//
LOCUS       DAOPYV010000039        27834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_1698_length_27834_cov_10.935275, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000039 DAOPYV010000000
VERSION     DAOPYV010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27834
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1698_length_27834_cov_10.935275"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1013
                     /locus_tag="PKI35_06675"
     CDS             108..1013
                     /locus_tag="PKI35_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44106.1"
                     /translation="MTDTTYILTQAAQTGGASHNDFIEGLKIAATLAAPFIASFLTYW
                     FAIRGKLKDSDIARRNELNTVLSNLLIVWHYLTKIEDTCKLKYDDKIALPIPKDYISS
                     LTLKSGTLNDKCFNELDDSILSLKKYDPISFYELEGIGRRLEFLRKNFILPLIKSKST
                     ANFNRVVAEKYLKLMIDELEEYIETIAEGLSNETLSKALDKLQRHFEKNTEKIKDELL
                     LEYYELIVAINPNNAPTYEQFVSEISKPEAQAELNKQFESIGNIDMDKLMEVVADNPN
                     ISMEEIVMEIDIREKNANNNDDNPL"
     gene            994..1245
                     /locus_tag="PKI35_06680"
     CDS             994..1245
                     /locus_tag="PKI35_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44107.1"
                     /translation="MMTILFETDCKTRQDGIRTDSRAAANSTYPKGGVSCSKNSFVEK
                     QTLVFQIKFCGKSPAFRVAANRYTQKNNLTGNSHYGAKD"
     gene            1229..1711
                     /locus_tag="PKI35_06685"
     CDS             1229..1711
                     /locus_tag="PKI35_06685"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNX44108.1"
                     /translation="MEPRIEMLSETLLIGKKAIMSFSDNKTVELWRSFSPRLREIKNP
                     AGNELYSVDIYNDTHFFNHFDPAKEFEKWAAIAVKNFDSVPEEMDKLILPKGSYAVFP
                     YRGKPSDAQKALQYIYGIWLPGSEYDMDDRPYFALMGEKYKGEHPDSEEEFWVPITNK
                     "
     gene            1864..2391
                     /locus_tag="PKI35_06690"
     CDS             1864..2391
                     /locus_tag="PKI35_06690"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX44109.1"
                     /translation="MIRLLDLQDYDAWIELAEEVEPLFGPMTDSKEFQNGIKDCILNK
                     NAFGLENEKGNLTGIVAIDRANNEIMWLAVAKKHRGNNYGEKLVKKAIEELESNGDIY
                     VQTFSGKVNIGKNARKIYESNGFADFKDAGRNPAGIETVMMIRKEKNTAPDKAVIKHS
                     GFGAICKLSSRRQGS"
     gene            2470..3231
                     /locus_tag="PKI35_06695"
     CDS             2470..3231
                     /locus_tag="PKI35_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX44110.1"
                     /translation="MAFFTFEDKKVYYKQIGSGEPLLLLAGNTASSKMFKSVTGKYSK
                     DFKVILIDFPGHGKSDRVEKFEVDFWYYNARVCYQLLDFLRLDKVSVIGTSGGALVAI
                     NLSLEHPERIKYLIADSFEGEYPLNSYIDSLESDREKGKKNIFAKMFWFLNHGFDWKK
                     IVDLDTEMLISFSKRGESFFHKSFAELTVPSLLTGSKQDEYCDSLDKIYEALKIKNDK
                     LEIHMFEKGKHPAMISNKNEFYELVKNRIITPDAV"
     gene            3414..3662
                     /locus_tag="PKI35_06700"
     CDS             3414..3662
                     /locus_tag="PKI35_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44111.1"
                     /translation="MTTSDMKLQLLRMLDSLDNSRLEELYRVVANFINGKKDLAEWEN
                     LPENQKQGILDAIGEIESGKGIPHSAIVKKYRKKYSHA"
     gene            complement(3954..6194)
                     /locus_tag="PKI35_06705"
     CDS             complement(3954..6194)
                     /locus_tag="PKI35_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNX44112.1"
                     /translation="MGELPELIIDLALILSVAALVTLIFKKLKQPVVLGYIIAGLLVG
                     PNFRLFPTVIEMDSIKTWADIGVIFLLFGLGLEFSFKKLLKVGGVAVITAITEVSLTM
                     LLGYAAGRLLGWNFVNSLFLGGILAIASTTIIVRAFDELGVKNQKFAGIVTGVLVIED
                     LVAVLLMVLLSTVAVSQTFAGGEMIKAVMELAFFLVLWFVSGIFFIPTFFRKTRSLLN
                     EETLLIISLAFCFLMVVLATYAGFSSALGAFVMGSILAETTKAEKIEHLTKSVKLLFG
                     AIFFVSVGMMIDPHMLIKHALPIVAATLVLLLGKPLFVTLGAMLAGQPLNIAVRSGMS
                     LSQIGEFSFIIATLGLSLKVTGEFLYPVAVAVSVLTTFTTPYMIRFSESVSKGLESVL
                     PEKWKKIFAAYNAEAQHITETSAWKKVLKSDLVNVAIFSVVIISLIIVSTQFLVPFFS
                     ANGWNKIIAVIVSLALLAPFLWALAFRRTAPRYYASVWLQSFHRKPLIVLEVSRIVLA
                     VFFIGFLFNQLYSPVAALTGAAVTCAVLLLFSGRIKAFYGKIESRFIENYNERESGKM
                     QHEEILTPWDTHISRFELNARFPFYGITLSELKLRERFGVNIVVIGRGDDVINVPTRD
                     ERLYPSDVLSVIGTDDQLNRFEAFLELSSGFANQTDKKQNVSLHHFIVSKNSPFAGKS
                     IRKSGIREQSRGLVVGVERNGERIINPESDFVFEAGDILWIVGNEMRIQVIMKEAAAL
                     AETAPV"
     assembly_gap    6641..6740
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6806..9937)
                     /locus_tag="PKI35_06710"
     CDS             complement(6806..9937)
                     /locus_tag="PKI35_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX44113.1"
                     /translation="MKITEISIKRPIYVIVLFSVLTILGYLGYRSLSAELMPKFTPPM
                     INVQIIYPGASPSEVENSLTRKVEDALSGMPGIDRIQSYSYEGMSMLIVSFTYGTDID
                     KSITDAQNLISSKMSQLPREILTPIINKLTVDDKAIITLSATSKLEATQFYDLMDKRI
                     IPELSGIRGTAKITMAGGQQREIQVNLDAQRMRAYGVSPAQVQGVIRAANLDFPTGSI
                     RNEETNTAVRLSGKFKSVEEIRNLVVFTTPSGSQIRLQDIAEITDAVKDPKNLARVNG
                     EEAILVNILKQGDANAVQVSDQVRKKIVELEDEYSAQGLSIDIAQDSTTFTRESISSV
                     LNDLLLAVLLVSVVMLLFLHNFRNALMVMVVVPVSLVATFIGMNLLNFSLNLMSLLGL
                     SLVIGVLVDDAIVVIENVYRHMEMGKNRVRASWDAIKEIGFTVLSITIVIVIVFLPIA
                     LTNTLVSDILRQFCLVIVISTLLSLLAALTLVPLLTSRFGTIHVLTGKNLFQKILMGF
                     ETGVTAFSNWIMKLMHWSLSHKRWVGLIILAITVAVFSLFPLGFIGFEFLPDVDRGEF
                     IVQMEMPKDISLEESNAMVRKAENWFRTRPEVKSVVTMVGLTSDNTQSSQGLPYVAEL
                     NVKLVPRDQRNEDTKLYITKIKAPLTDYLVDARVKIFSVSLTGTAAKAAVEYVITGTN
                     SDSVMVFAEKALQVMRSIPGTMQQELSVESGTPEINVAVDRDKMSALGLTLDNVGFTM
                     QMTYQGNNTLKYTEGNYEYDINIRADKAYRRQVEDVEDLTFTNSRGETVRLSQFADIK
                     LGTGPNRLERYNRNSSVTIRSQVLGVSAGAVSKEFTNRLSKMDMPRGVKLVVTGDMKN
                     MGESMGVLTGALLLSLLLVYLAMVVLYNNWTDPFVVMFSIPLSVIGAILALALTNTAL
                     SIYGMLGLVMLIGLVAKNAILLVDFANDAIGEGKSINDALTQAVRIRTRPILMTSLSV
                     IIGMLPVALSRGAGAELRNGLAWVIIGGMLVSTFLTLVVVPVMYKIMHSLRRSVKDRP
                     DIEKLMREEVRSN"
     gene            complement(10016..11071)
                     /locus_tag="PKI35_06715"
     CDS             complement(10016..11071)
                     /locus_tag="PKI35_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX44114.1"
                     /translation="MKRFISIISVVAVIAGIAMILVLNKKSTSEKTSLASQVSTAVPV
                     KADVVRDEAFNQSFTSNGSLEPERELSFVSDVAGRVLNVYADEGTVISGGQVLVQVDD
                     EMLRADFMASEASYNALKSDLERFTNANKQGGVTDQQLETIRTQCIAAESRYISSKRR
                     LSDARIKAPIRGIINKRYVEVGTFLNPGARLFDIIDDSKLHVWCNVTERQVLLIKKGQ
                     KVRILCSTFPEDSFSGTITFIGEKADRALSYPVEITLNREGKSLLKAGMYVTACFDMQ
                     SEKQGIVIPRSAITGSVKNARVYVVENGTATGRDVVVGMMMDKDVEILKGLQAGDSIV
                     VAGLINVSDGAKVRNIK"
     gene            complement(11179..12546)
                     /locus_tag="PKI35_06720"
     CDS             complement(11179..12546)
                     /locus_tag="PKI35_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX44115.1"
                     /translation="MGNRKKLMLCFLLFACSNLMGQKAYNLQSCIRYALENNNNIRKT
                     HLDVEKSAQARREVTGALLPQISGSGSLNDNFQKAKFIMPNFMKEFLPPSMLDPNASD
                     YMTIEMGLNYSTAIGAGLNQQILNFSLFNALDIAKTAESLSALAVDAKEEDVIAQTAN
                     LFYAVQVTGYAVEMFGRSINIIDSMLTTMEVSYANGLVKKVDLDRLKVTKTNLMTQRT
                     AMQSAIGVQKNLLKLQMGLDVNKPVEIEKINLEIFENQLKSPYEQAFSLNSQTAYKIL
                     EKQAGLTQLKRKSAVYESYPVLTAMASYNYNGVSNKFFTGETTYWYPTSMVGLSLRVP
                     IFGGLSRSAKIKQADIEIQKVGEDVVSLGQTLNMAFNNALLKLEDNRRTIQVQRDNMA
                     LAEDVYRISESNFSQGLASMTDVLNASNSLIQSQMNYADALGNFMKSWIELQKSNGTI
                     RSLVE"
     gene            complement(12572..13174)
                     /locus_tag="PKI35_06725"
     CDS             complement(12572..13174)
                     /locus_tag="PKI35_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX44116.1"
                     /translation="MTGNRKAKYAEKALETFKQDGLRLSLDEISEKIGVTKKTLYNHF
                     DSKEELLSECIHTFVLDLKQRMSIMTSADVNAVDGLKCGVDEIAGFFRTLSPVFLSDI
                     KKMYPEIANTEHTTGFAFFLEGVKLNLLKGIEEQLFRNDLNVELISQYFTFSLVSFFI
                     SRVANSSAFLAGDYFNSILDYHLQAIVTEQGRKLLNSTSR"
     gene            complement(13560..15551)
                     /locus_tag="PKI35_06730"
     CDS             complement(13560..15551)
                     /locus_tag="PKI35_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNX44117.1"
                     /translation="MKYWNYPAQGTGFHSYNHPTYGTLSANFAATTYDWGAMPNSLTG
                     PNLAIATLMYHCGVAVEMDYGVDGSGSYVIIAASPTPEQCCEYAYKTYFGYDPSTLQG
                     LKRANYSDNEWKQLIKNDLDQGRPVQYAGFGAGGHTFVCDGYDVNGYFHINWGWSGIA
                     DGYFLLDALNPGAGMGTYNSGQQAVIGIKPLTGGTTYNISLYDNVIASPNPVGYNQSF
                     TIHTDIANYSTSTFEGDFCAAVFDENVDFVSFVEILSGCSLPGNSHYTNGLDFTSSGS
                     LSLLPGAYYIGIFFRPAGGDWIMVDDGSYSNMISFAVNNSNDIELYQEMVLSCGDNII
                     QEQPFTVTLDVANYGTATFNGMFDVSLYNADSVGSFAENIATLTGASLEPGYFYDDLQ
                     FSTDGVSVSPGTYLLALTHKEEGGNWQLTGSTYHPNPITVIVQEPGIIPDSYENNDSL
                     AAAYALTLNAYHVLHITATTTGSNIHKGSDRDYYKIVLPSGFDYSVTARAQDSYSSNN
                     GYIYSCDVVWLYLAGSTWSDAFDDVMSGSINVTDGGTVYFQVVPYFAGETGSYLLDID
                     ISKSAGGGIKEAASSGFLSVFPNPADDVLYIRTEKDINISNLKIFDALGRLVAEVNNP
                     AGQNHCIAVPVSNLATGAYVLLLQTDGSVWQQKFVKTAP"
     gene            complement(15548..16147)
                     /locus_tag="PKI35_06735"
     CDS             complement(15548..16147)
                     /locus_tag="PKI35_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spi family protease inhibitor"
                     /protein_id="HNX44118.1"
                     /translation="MKKFLLLIVVCTGMLKLSDAKEVDTETARLVAKNLYQQVVKEQG
                     GIYLELAGTSVYKFNTHLNTPDSVPLFYVFNEVNHHGFVIVSGDDNAIPVLGYSASGS
                     FDESKLPPNFRKWLEGYKNQLREIITKKMDATGEIRSEWQRLKTGQPLNENRNITAVT
                     PLLTTTWDQSPYYNDLCPYDDTYNALTVTGCPATAMARS"
     gene            complement(16655..16738)
                     /locus_tag="PKI35_06740"
     tRNA            complement(16655..16738)
                     /locus_tag="PKI35_06740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16702..16704),aa:Leu,seq:cag)
     gene            complement(16757..16943)
                     /locus_tag="PKI35_06745"
     tRNA            complement(16757..16943)
                     /locus_tag="PKI35_06745"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16907..16909),aa:Leu,seq:gag)
     assembly_gap    16788..16887
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17072..18643
                     /locus_tag="PKI35_06750"
     CDS             17072..18643
                     /locus_tag="PKI35_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HNX44119.1"
                     /translation="MFQTKEVKNMFTKDDLKQLQSHGIDPKTAELQINNFVKGFPYIN
                     LDRPAIVGDGIKAFNAKEAKKLSYFYDSNSKKYEILKFVPASGAASRMFKHLFEFSET
                     YNGTEKDITGLLNDNQLLPVKQFFDHIKEFAFAKALSKAMRKDDFDLDDCLARNDYKP
                     IIDYLLTEKGLGYANLPKGVLLFHNYPDEPRMAIEEHLVEGANYCKDKEKRAGIHFTV
                     SPEHADRFLDEINRVKEKYEELYDVTYELTFSLQKTSTDTIAVDLNNKPFRDANGKLV
                     FRPGGHGALIENLNDRESEIVFIKNIDNVVPNHPKEDTFLYKKVLGGYLFNLQDSIFE
                     FIEILEDKPDDEDIRTILRFIKTKLGFAIDPDFSAWRKEDKVEYLHRKLNRPIRICGM
                     VKNEGEPGGGPFWVKEANGEISLQIVESSQIDLNDEKQKAILNQSTHFNPVDLVCGVR
                     DYKERNFDLRNYVNPSTGFISIKSKDGKSLKAQELPGLWNGAMSDWITVFVEVPVSTF
                     NPVKTINDLLRPEHQ"
     gene            18995..19552
                     /locus_tag="PKI35_06755"
     CDS             18995..19552
                     /locus_tag="PKI35_06755"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNX44120.1"
                     /translation="MSHSQRTAYIKELDGERKQKDEEMRTAEKSPLPADAKAGFRGLN
                     YFGGSPEYQVKAILKRFDDPITFSMKTTTDRLPEYRLFGEASFTLKGEPWVLEVYQNV
                     ELSKKPGYADYLFIPFNDRTNGRATYGGGRFIDARIPEGDTLIIDFNRAYNPYCAYNH
                     KYSCPVPPDKNNLETKIKAGEKKFH"
     gene            19583..21418
                     /locus_tag="PKI35_06760"
     CDS             19583..21418
                     /locus_tag="PKI35_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44121.1"
                     /translation="MASKSSSVEFETYRSLRHFRNFLRRPKYPHATVNRIVPHVAWII
                     LFWLAAYLYKERIFADSGFYFSQFVNNKTFWIECQRIVLAFSQIIPLAGVWAGIPLQY
                     VLLLYSLSHVAFFYFLFIFVYYGMRDRRSGLLIILSQTVGIMYSFFTPMFELYYGVPL
                     LITFYAFWRLELDRPFITLILIILEILLLLSHPVAFILFVFLLLYDIPERRESRKPLY
                     LYILVALVYVGVVIYKGFTMCEYETGKLAWQFNFTENKTYLQLLDPAYYNTMALFFLK
                     YYWEVIAALLVVTITLLKQRQWFRLLLTYGFLASYLFLVNSAYAVSQSRYMEQVMFPV
                     VPIVFIPLIYSFPKEPRPGIKNISVFLIGALIAYRLFIIYEGSHIFVQRTRQMESLIS
                     SARQMGGSKFYVYPQNIDRGYTQFNWSYPLETLILSSIRGNNYSVTIAPFDDLEFQDN
                     IRKMGANDFLFRRWEIKDKSWLNSRYFHLDPGLYRTMNDQSPNDDLEYMARNFRIAIE
                     TRNYYNSLDTVWMKVHITNTGTDILHTGSKNKIFLSYFWKKDGDVLNWTNIQTPVESD
                     IVHSLTQDMLVAVPSVKGKLQLQVDIISVDKQWFGLNATKDILVY"
     gene            21452..22879
                     /locus_tag="PKI35_06765"
     CDS             21452..22879
                     /locus_tag="PKI35_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HNX44122.1"
                     /translation="MNAKRILILTILLLTAGIIHAAPKGYRIIVKLHNSTDTVLLMAH
                     YFGDKQYLDDTAFVGKQGTYIFEGDEPLKEGMYIVAGQNKSRYFDFFISGPQQFELDC
                     DPNDVTGTMKVKGSEENKVFNEYIRYLAEKQKEIEPLNRQLKSLKPGSDSAVLINKQI
                     EETDKEVKAYMSNLFSKYTGLLSVKFVKANNEPDITPFLKDQNGKVDSTRLYPVYKQH
                     FFDNFTFGDPRLIYTPVFTARIDRYLQSLVVPTADSLQTAIDHLLSLASADKETQKYL
                     AWYLAVKYETSEIMGHDALFVYIVKKYLEPGKIEWYYPEVKDRVTKRAGTLEPLLLGK
                     QAPDLILLDTLNLPHSLYSVKAPYTLLFFWESTCGHCKQEMPNVLKFYEEFHKDYGLE
                     IYGVSGDTSMVKWKEYIRSNNMPWISVNGYLSLKGNYHDIYDIHSTPVMYLLDENKKI
                     LAKFLLSEQMGDLIKKREGARKKDE"
     gene            complement(22980..23324)
                     /gene="rplT"
                     /locus_tag="PKI35_06770"
     CDS             complement(22980..23324)
                     /gene="rplT"
                     /locus_tag="PKI35_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006044808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNX44123.1"
                     /translation="MPRSVNAVASRARRKKVLKAVKGQFGRRKNVWTVAKNAYEKGGQ
                     YAYRDRRTKKRNFRALWITRINAGVREYGMSYSVFMGKLHEKNIELDRKVLADLAMNN
                     PEAFKAIVELVK"
     gene            complement(23409..23603)
                     /gene="rpmI"
                     /locus_tag="PKI35_06775"
     CDS             complement(23409..23603)
                     /gene="rpmI"
                     /locus_tag="PKI35_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNX44124.1"
                     /translation="MPKMKSKASAKKRFTTTGTGKLKRRHAYKSHILTKKSTKRKRNL
                     GYVAIVDKADVKAVKDMLQC"
     gene            complement(23777..24310)
                     /gene="infC"
                     /locus_tag="PKI35_06780"
     CDS             complement(23777..24310)
                     /gene="infC"
                     /locus_tag="PKI35_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNX44125.1"
                     /translation="MDRRYPRKQKEELHQINERIKAPVVRVVGENITPGIYNIKEALM
                     IAEGLEMDLVEISPTANPPVCKVIDYKKFLYELKKKQKEIKAKTAKIVVKEIRLGPQT
                     DDHDFNFKLKHAQKFLQEGCKVKVEVFFRGRSIVYKEQGEIIMLKFAQELMEYGKLEK
                     LPMLEGKRMIMIISPKK"
     gene            complement(24468..26402)
                     /gene="thrS"
                     /locus_tag="PKI35_06785"
     CDS             complement(24468..26402)
                     /gene="thrS"
                     /locus_tag="PKI35_06785"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNX44126.1"
                     /translation="MINVTLPDNSVRQYPKGTTSLEVALSISEGLARNVLAAKVNGEV
                     WDATRPLTSDARLQLLTWNDTEGKATLWHSSAHLMAEAIEQLYPGVKFGIGPDIENGF
                     YYDMDFGERQLTPEDLPAIEAKMLELAREKQTFRRREVSKAEALDYFTKKNDEYKLEL
                     INDLEDGHITFYETGSFTDLCRGPHLPDTSSIKAVKLLNIAGAYWRGDEKRKMLTRVY
                     GITFPKQKELDEYLVMIEEAKKRDHRKLGRELELFTFSQKVGQGLPLWLPKGAMLRER
                     LERFLKNVQQEAGYQPVITPHIGNVELYKTSGHFQKYGKDSFQPINTPIEGEQFMLKP
                     MNCPHHCEIYNSKPRSYKDLPLRFAEFGTVYRYEQSGELHGLTRVRGFTQDDAHLFCR
                     PDQLLDEFKGVIDIVLHIFRVLDFKEYIAQVSLRDPNNKEKYIGTDENWEKAESAIIE
                     AAKEKGLKTIVEIGEAAFYGPKLDFMVKDALGRKWQLGTIQVDYNLPERFELEYTGSD
                     NQKHRPVMIHRAPFGSMERFVAVLLENGGGNFPLWLTPDQVTILTISEKYLDYANKVA
                     GILKKSEIRALVDERNEKTGKKIRDAELLKVPYMLIVGEKEEQNGTVSVRKHGQGDLG
                     TMSIDEFIVLINQEIKQMLD"
     gene            26673..27701
                     /locus_tag="PKI35_06790"
     CDS             26673..27701
                     /locus_tag="PKI35_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44127.1"
                     /translation="MKTKFTFEHSGLLFALLPLTGILLTLNLACKKNTLSELAPNNPL
                     NYTLKDEELIDSVYASDGRLVFTSLAEYNLFLNDIGTLNDSSLRVIEGEYNYTSLRES
                     VNYDDSFLPEEDKVLGTIFNSHSIFQVEHWVLKLDGDTVYAVYDYLYNEDSEGCWTDE
                     SVIKVSENQDVWFETGLDNEDCNETNRSDQTVKDTFYLITATANNSNSYQYGFELKLK
                     YNTSCFGLSHKYIIYGTFGCYYNGGFQSLASGGTYTLLPASPFSMKLNEYYATLDPRC
                     KSCKTYQKTNELFIYRFDQTLYSSMRGLQYHKGKVKCDLTIGSNTKSTPFLTMESWHP
                     SSCDCPTW"
BASE COUNT         7648 a         6471 c         6086 g         7429 t
ORIGIN      
        1 gtgtcccggc cgtgaaagaa tccatgaaaa gtggtaagtt tgtgtggata tatttagtta
       61 gctgcaaccc tatggcgaca cagcaaaccc gaaactaact gcaaaaaatg accgacacaa
      121 catacatact tacacaagct gcacagacag gaggagcatc gcacaacgac ttcattgaag
      181 gtttaaaaat agcggcaact cttgcagctc ctttcatagc ttcgtttctg acttattggt
      241 ttgcaattag aggtaaacta aaggactcag atattgcaag aaggaatgag ctaaatacag
      301 ttctctctaa tttgcttatt gtttggcatt acctcacaaa aattgaagac acctgcaaac
      361 tgaagtatga cgacaaaatt gctctgccga taccgaagga ttatatttca tctctgactt
      421 tgaagtcagg aactttaaac gacaaatgtt ttaacgaact tgatgattct attttaagtt
      481 tgaaaaaata cgacccaata agtttctatg aattagaggg tattgggagg cggttagagt
      541 ttcttagaaa aaactttata ctcccattaa ttaaatctaa gtcaacagca aattttaata
      601 gagtagttgc agaaaaatat cttaagctga tgattgacga acttgaagaa tacattgaaa
      661 ctattgccga aggacttagc aacgaaactt tatctaaagc ccttgacaaa cttcaaagac
      721 attttgaaaa gaacacagag aaaattaaag acgaattact tttggaatat tacgaactga
      781 tagttgcaat caatccgaac aatgccccaa catatgagca gtttgttagt gaaataagca
      841 agccagaagc acaagcagaa ttgaataagc aatttgaatc aataggcaat attgatatgg
      901 ataaactgat ggaggttgta gccgacaatc caaatatatc aatggaggaa attgtaatgg
      961 aaatagatat tcgtgaaaaa aacgcaaaca acaatgatga caatcctctt tgaaactgat
     1021 tgcaaaaccc gacaagacgg aatccggacg gatagcaggg cagcagctaa cagcacctac
     1081 ccaaaaggcg gggtttcatg ttccaaaaac agttttgtgg aaaaacaaac attagttttt
     1141 caaatcaagt tttgtggtaa aagtcccgcc tttcgggtag cggcaaaccg ttatacgcaa
     1201 aaaaataact taaccggaaa ttcacattat ggagccaagg attgagatgc taagtgaaac
     1261 cctgttaatc gggaaaaagg cgatcatgtc attttcagat aacaaaaccg tcgaactatg
     1321 gagaagtttt tcgccacgac tcagagaaat taagaatccg gcaggaaatg agttgtattc
     1381 tgttgatatt tataatgata ctcacttttt taaccatttc gatccggcaa aggaatttga
     1441 gaagtgggct gccattgctg taaaaaactt tgattcagtc ccggaagaaa tggacaaact
     1501 gatcctccca aaaggttcgt acgccgtatt tccctaccgg gggaaaccca gtgatgcaca
     1561 aaaggcactt cagtatattt atggcatctg gctccctggt tctgaatatg atatggacga
     1621 caggccctat ttcgctctta tgggagaaaa atacaaaggc gaacatcctg attccgagga
     1681 agagttttgg gtacccatca cgaataaata agcctgcacc tgataaaaag catatgaacg
     1741 acgcatcccg tccatcggca gtcaggcggc tgttcccggc tgaaccgcat cctgacgagg
     1801 ccggcgctgt tttttttaac cgcgttccgg ccgaaattga taaattaaaa aggaataaac
     1861 aatatgatta ggttactcga tttgcaggat tatgatgcct ggatagaact tgccgaagaa
     1921 gttgagcctt tattcggacc catgacagat tcaaaagaat ttcaaaatgg aataaaagat
     1981 tgtatactaa ataagaacgc atttggtctc gagaatgaaa aaggcaattt gacaggaatt
     2041 gttgctatag acagagcaaa taatgagatc atgtggttag cagtagcaaa gaaacaccgc
     2101 gggaataatt atggagaaaa gttagtaaaa aaggcaattg aggaattgga aagcaatgga
     2161 gatatctacg ttcagacttt ttcagggaaa gtgaatatag gaaaaaatgc caggaaaatt
     2221 tatgaaagca atggatttgc agactttaaa gatgccggaa gaaaccctgc tggtattgaa
     2281 accgtgatga tgataagaaa agaaaaaaac actgcgcccg ataaagcagt cataaaacat
     2341 agcggtttcg gtgcgatttg caagctcagt tcccgccgtc aaggttcgta acgggtgata
     2401 ggttcgcaac cacgcaatcg gccatgtttc acaccatcac cgttatgggg tacctaaaaa
     2461 agaaggcata tggcattttt tacatttgaa gacaagaaag tctattataa acagatcgga
     2521 tcaggggagc cgttattatt attagcagga aatacggctt cttcaaagat gtttaaatcc
     2581 gtaaccggca agtactctaa agatttcaaa gtgattctga ttgatttccc ggggcatgga
     2641 aaatcggaca gagttgaaaa atttgaggtt gatttttggt attataacgc aagagtgtgc
     2701 tatcagttat tggattttct aaggctcgat aaagtttcag tgattgggac cagcggaggc
     2761 gccttagtcg caattaattt aagtttagag catccggaaa gaataaaata tttaatcgca
     2821 gatagttttg aaggggaata tcctttgaat tcttatattg actcattgga aagcgacaga
     2881 gaaaagggaa agaagaacat atttgcaaaa atgttctggt ttttgaatca tggttttgat
     2941 tggaaaaaaa tagtggacct ggatacagag atgctgatca gtttttctaa acgaggcgaa
     3001 tcattttttc ataagtcatt tgcagaacta acagttccat ctttactgac gggaagtaaa
     3061 caagatgaat attgtgattc gttggataaa atatatgaag cattgaaaat aaaaaacgat
     3121 aaacttgaaa ttcatatgtt tgaaaaaggg aaacatccgg caatgatatc caataagaat
     3181 gaattttatg aactggtaaa gaatagaatc ataacgccag atgcagtata gccaataagg
     3241 gtttcagatg tataagatgg tagtaacacg cttgtacttt ccgtattcct gacaggaaat
     3301 cgcacgcaac agcgaagcgg aataatccct tgttgttgct gcactcaatc tttcaaaggt
     3361 ttgtttacag cctgcttttg tatatatctt gtaacattgt aaaaaataaa acgatgacaa
     3421 cctcagatat gaagcttcag ttgttacgca tgctggattc tcttgataac agcagactgg
     3481 aagaattgta tagagtagtt gccaacttta taaatggcaa gaaagacctc gctgaatggg
     3541 aaaacttacc ggaaaatcaa aaacagggca ttttggatgc catcggggag attgaatcag
     3601 gaaaaggcat tcctcattca gcaatagtga agaaataccg taaaaaatat tctcatgcct
     3661 aagcatgtag tttggtcccc ttgctgaaaa agacttcgaa aatattcttg agtattaaat
     3721 gataattggg acaaccgggt cgcaaatagt tatattgatg cacttaaagc cggaatttgg
     3781 tgttttcctg aaatgatttg acggggtgtt ggcgggataa aatgtcgttg tttccggttt
     3841 cggcgccctc gccccctagc ctgcgtcgtt atctgatccg tgacgaatat tccggcttgt
     3901 tttcatatcc atttggtatc acgttaactc attacgtaaa aactttaaag tttctatacc
     3961 ggcgccgttt ccgccagcgc cgccgcttct ttcatgatca cctggattcg catttcattg
     4021 ccgacgatcc agaggatatc ccccgcttcg aaaacaaaat ccgattcggg attgatgata
     4081 cgctccccgt tcctctccac ccccactacc agtccgcggg actgttccct gatgccggat
     4141 ttccggatgc ttttcccggc aaacggagaa tttttgctta cgataaaatg atgcagtgat
     4201 acgttttgct ttttgtcggt ctggtttgca aagcccgatg acaattcaag aaatgcctcg
     4261 aatctgttca actggtcgtc ggtgcctatt accgacagca catccgaagg atacaggcgc
     4321 tcgtcgcgcg tggggacgtt gatcacatca tcgccgcgtc ctatcacgac gatgtttacc
     4381 ccgaaccgct ccctcagttt taattccgaa agcgttatcc catagaacgg gaaccgcgca
     4441 ttcagttcga acctcgaaat atgagtatcc catggcgtga gtatctcctc atgctgcatc
     4501 tttcccgatt cccgctcatt atagttttcg atgaagcggg attctatttt tccgtaaaac
     4561 gctttgatcc ttcccgagaa aagaagcaac accgcgcagg taacagcggc acctgtcagc
     4621 gccgccaccg gcgagtacaa ctggttgaaa agaaaaccta taaaaaaaac cgccagcact
     4681 atcctcgaca cttcaaggac gatcaacggc ttgcggtgaa aagattgcag ccaaacgctc
     4741 gcatagtaac ggggtgccgt cctgcgaaag gcaagcgccc agagaaaagg cgccagtaac
     4801 gccagcgata ctattacggc tatgattttg ttccagccgt ttgccgaaaa gaaaggaacc
     4861 aggaattgtg tgctgacgat aatcaggctt atgatgacca cggaaaaaat ggcaacattc
     4921 acaagatctg atttcagcac ttttttccag gcgctggttt cggtaatatg ctgcgcttct
     4981 gcgttgtagg cggcaaaaat ctttttccac ttctccggca aaacggattc caggcccttg
     5041 ctgacagatt cggaaaagcg gatcatgtaa ggggtggtga aagtggtaag caccgataca
     5101 gccacggcta ccggataaag gaattcgccg gtcaccttca ggctgagacc cagggtagcg
     5161 atgatgaacg aaaactcgcc gatctgggaa aggctcattc ccgacctgac tgcaatattc
     5221 aggggctgcc cggccaacat ggctcccagt gttacaaaca gcggttttcc caacagcagc
     5281 acaagggtgg ccgcaacaat cggcagagca tgttttatta acatgtgcgg atcgatcatc
     5341 ataccgaccg agacaaaaaa gattgccccg aaaagcagtt taaccgattt ggtaagatgt
     5401 tctatcttct cggctttggt ggtctcggca aggatggaac ccatgacaaa tgcgccgagc
     5461 gccgatgaaa agccggcgta cgtggccagc accaccatca gaaagcaaaa agccagggag
     5521 atgatcagca acgtttcttc gttcagcaac gaccgtgttt tcctgaagaa tgtaggaatg
     5581 aagaagatgc cggagacgaa ccacagtacc agaaaaaaag ccagttccat cactgctttg
     5641 atcatttcgc ccccggcaaa tgtctggctc acggcaacgg ttgaaagcag taccatcagc
     5701 aaaacagcga ccaggtcttc gataaccagc acgccggtca cgataccggc aaatttttgg
     5761 ttcttcacgc ccagttcatc aaacgccctg acgataatag tagtagatgc tatggccaat
     5821 atcccgccca ggaaaagact gttgacgaaa ttccatccca gcagccttcc ggcggcgtaa
     5881 cccaggagca tggtcagcga tacttcggtg atcgccgtta tgaccgctac cccgcctacc
     5941 tttaacagtt tcttaaaact aaactcaagg ccgaggccga acagcaggaa gataacgccg
     6001 atatcggccc aggtcttgat gctgtccatt tcgatgaccg tagggaacag cctgaaattg
     6061 ggcccgacaa ggagcccggc gatgatatac cccagcacca ccggctgctt taatttttta
     6121 aagatcagcg taaccagtgc ggcaacactc agaataaggg ctaaatcgat tatcagttca
     6181 ggtaactctc ccatgataca gtccggatca gtatgcagca gtcaacgttg ccatgaacaa
     6241 ggcaaccgca tcggaattat tcttccggcg cctgatacgc tgcttgggta aagataaagt
     6301 tttattcttt catcatgccc ggctgtcctg attttttaag atcaaggaag gcaagccgga
     6361 aacggtaaaa cggcgttatt tataaaaggc gaacgacata tgccagcacc tgcagagcca
     6421 tgatttgctt tacactgaaa acggaagccg ggtattccgg tcggaaccag gaatccggcg
     6481 agtccggcag ggatgatcag aaaggtttgt ttgccagggt cagggcagcc ccgaaggggc
     6541 tgaatatcca caaaaagcct gtaccttgta gccccgaagg ggctaaatct caataagata
     6601 catgtaccta tacagccccg aaggggctga atctcaataa nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn ccccgaaggg gctgaatctc aataataaga tgcatgtacc
     6781 tatacagccc cgaaggggct gaatctcaat tagagcgaac ttcttcccgc atcagctttt
     6841 caatatcagg tctgtctttc acactccgcc gtaaagaatg catgatctta tacatcaccg
     6901 gaaccacaac caacgtcagg aaggtcgaca caagcatccc gcctatgatc acccatgcca
     6961 ggccattcct caactcagct cctgctcccc ttgaaagcgc taccggcagc attccgataa
     7021 taacggaaag cgaagtcatc aggatgggac gggtacgaat cctgaccgcc tgcgtcaggg
     7081 catcgttgat agatttcccc tcaccaatgg catcgttggc aaagtcgacc agcaggatgg
     7141 cattcttcgc caccagcccg ataagcatca ccagtccgag catgccgtaa atacttagag
     7201 cggtattggt cagcgccagg gccaggattg ccccgatgac cgaaagggga atggaaaaca
     7261 tgaccacgaa cggatcggtc cagttgttat aaagcaccac catagccagg tataccaata
     7321 gcagcgacag cagtaacgcg ccggtaagca cccccatcga ttcgcccatg ttcttcatat
     7381 ctcctgtaac gacaagcttt accccccggg gcatatccat tttactcagc ctgttggtaa
     7441 attcctttga tacggcgccg gctgagaccc caagaacctg cgaacggatg gttacggagc
     7501 tgttgcgatt gtacctttca agcctgttgg gaccggtacc gagtttgata tcggcaaact
     7561 gcgaaagccg cactgtttcg ccccttgaat tggtaaatgt aaggtcctca acatcttcaa
     7621 cctgccggcg gtatgctttg tcggccctga tgttgatatc gtattcgtag ttaccctcgg
     7681 tatatttcag cgtattattc ccctggtaag tcatctgcat ggtaaagccg acgttgtcga
     7741 gcgtcaggcc gagagccgac atcttatcgc ggtctaccgc cacgttgatt tccggagtgc
     7801 cgctttcaac cgacagctcc tgttgcattg tacccgggat ggaacgcatc acttgcaatg
     7861 ctttctcagc aaaaaccatc accgaatcgg aattggtgcc ggtgatcacg tattccacgg
     7921 ctgccttggc cgctgttccg gtcaggctta cgctgaatat tttaaccctg gcatccacca
     7981 gataatcagt caatggcgct ttgatttttg ttatgtaaag ttttgtatcc tcgttgcgct
     8041 gatcgcgggg caccagtttt acattcagtt cggcaacgta aggcagcccc tgcgaacttt
     8101 gcgtattgtc gctggtaagg ccgaccatgg tcacaacgct cttcacttcc ggacgggtgc
     8161 ggaaccagtt ttcggccttc cggaccattg cattggattc ttcgagcgaa atatccttcg
     8221 gcatttccat ctgtacgatg aattcgcccc tgtcgacgtc gggcaggaac tcaaatccta
     8281 taaatcccaa cgggaacagg gagaaaacgg ccactgtaat agccaggatg atcagtccca
     8341 cccagcgttt atgcgaaagc gaccagtgca tcagcttcat aatccagttg ctgaaagcag
     8401 taacgcctgt ttcaaagccc attaatattt tctgaaataa gtttttcccc gtcaggacgt
     8461 ggatcgtccc aaaccgcgat gtcagcaagg gtaccagtgt caatgcagcc agaaggctca
     8521 gcagcgtgga tatgacaatc accagacaga attgccgcaa aatgtcggaa accagcgtat
     8581 tggtgagtgc aatgggaagg aaaacgatta cgatgactat ggttatggaa agcactgtaa
     8641 agccgatttc tttaatggca tcccaggatg cccttacccg gtttttgccc atttccatgt
     8701 gccggtaaac gttctcgatg accacaatgg catcgtcgac cagcacgcca atcacaaggg
     8761 aaaggccgag aagcgacatc aggttaagcg aaaaattgag caggttcatc ccgataaagg
     8821 tcgccaccag cgataccggt accacgacca tcaccataag ggcattgcgg aagttatgca
     8881 ggaagagaag catgaccaca gagaccagca acaccgccag caacaagtcg ttcaataccg
     8941 acgaaatcga ctccctcgta aaagtggtgg aatcctgggc gatatcgatg gataatccct
     9001 gagcactata ttcatcttcc agttcaacaa ttttcttcct aacctggtcg ctcacctgca
     9061 cagcgttggc atctccctgt ttcagaatat tcacaaggat ggcttcttcg ccgttcaccc
     9121 gggccaggtt tttcggatcc tttacggcgt cggtgatctc ggcaatgtcc tgcagacgaa
     9181 tttgtgaacc ggaaggggtt gtaaaaacca ccaggttgcg gatttcctcc acgcttttaa
     9241 atttccccga gagccggacg gcggtattag tttcttcgtt gcggatactc ccagtcggaa
     9301 aatcgaggtt ggctgcacgg ataacgccct gcacctgggc cggtgaaaca ccgtatgccc
     9361 gcatacgctg tgcatccaga ttgacctgaa tctcacgctg ctgaccgccg gccatggtaa
     9421 tcttcgcggt tcccctgatc cccgacagtt cggggatgat gcgcttatcc atcaggtcgt
     9481 aaaactgcgt ggcttcaagt ttggaagtgg ccgataaggt gatgatggct ttgtcgtcga
     9541 ccgttagttt attgatgata ggcgttaaaa tttcgcgcgg gagttgcgac attttcgaac
     9601 tgatgaggtt ctgggcatcc gtaatggatt tatcgatgtc ggttccatag gtaaatgaaa
     9661 cgataagcat cgacatgcct tcatatgaat aggattgaat cctgtcgatc cccggcatgc
     9721 ccgaaagcgc atcttccact ttacgcgtca gcgaattctc aacttccgat ggagaagcgc
     9781 ccggataaat aatctgaacg ttaatcatcg ggggcgtgaa cttcggcatc agttcggcgc
     9841 tcaggcttct gtatcccaga tagccgagga tggtgagcac cgagaagagt acaattacat
     9901 atattggacg cttgatggat atttctgtaa ttttcatttt tctttctttt gaggaagtct
     9961 gaacgccggg ttttaaggcc tgcgcatttg attctccggt tcctggtttg aactgttact
    10021 taatattccg gactttagcg ccatccgaca cattgatcaa accggctact acgatgctgt
    10081 cgccagcctg taaacctttc aggatttcaa catctttatc catcatcatt cccacaacaa
    10141 catcgcgtcc tgtggcagtt ccgttttcca caacatagac cctggcgttt ttcacgctgc
    10201 cggttatggc gcttctcggg atcacaatac cctgcttttc cgattgcata tcgaaacagg
    10261 ccgtgacata cataccggct ttcagcaaac tcttcccttc ccggttgagg gtgatttcta
    10321 ccggatagct gagcgccctg tctgcctttt ccccgatgaa tgtaatggtt cccgaaaaag
    10381 aatcttcagg aaaagtgctg cacaggattc ttaccttctg tccctttttt atcaatagca
    10441 cctggcgttc ggtaacgttg caccacacat gcagttttga atcgtcgata atatcgaaca
    10501 ggcgagcgcc gggattcagg aatgtaccca cctccacata ccgcttgttg atgattcccc
    10561 tgatcggggc tttgatcctg gcgtcggaaa gacgacgctt gctgctgata taacggcttt
    10621 cggctgctat acactgcgtg cgtatggttt caagctgctg atcggtgacg ccgccttgct
    10681 tattggcatt ggtaaaacgt tccaggtcgg atttcaaggc attgtaactg gcctcgctgg
    10741 ccataaaatc ggccctgagc atttcatcat caacctgtac cagaacctgt ccgcccgaga
    10801 ttacagttcc ttcatcggca taaacattca acacacgccc tgccacatcc gaaacaaacg
    10861 aaagctccct ttccggctcc agggacccgt tggatgtgaa actctggtta aaagcttcat
    10921 ccctgacaac gtccgcctta accggaacag ccgtggatac ctgggaagcc aggctggtct
    10981 tttcactggt ggatttctta ttcagcacca ggatcattgc aatacctgcg attactgcaa
    11041 ctactgaaat gatggaaata aaccttttca tattgttggg gtattattgc tgacagaatc
    11101 cttttgctat cggggattca gttccagctt gattaatgaa ttattttgct tcttttctat
    11161 tatccggagc gccttcggtt actccaccaa cgaacggatc gtcccgttgg atttttgcag
    11221 ttcaatccac gatttcatga aattgccaag ggcatcggca taattcatct gcgactggat
    11281 gagcgaattg ctggcgttga gcacatcggt cattgatgcc aatccctgcg agaaattact
    11341 ttcggagatg cgatacacat cttcggccag cgccatatta tcgcgctgaa cctgtatggt
    11401 cctgcgatta tcttccagtt tgagcagggc attgttgaag gccatgttaa gcgtctgacc
    11461 aagcgaaaca acatcttcac caactttttg tatctcgata tcggcctgtt ttatctttgc
    11521 gctccgcgaa agacctccga aaatgggcac ccgaagcgac aagccgacca ttgaagtggg
    11581 ataccagtat gtggtttcac cggtgaaaaa tttattcgac acaccattat aattatagct
    11641 ggccatggcc gtcagaacgg gatatgattc ataaacggca gattttcgct ttagctgcgt
    11701 taaaccagcc tgcttctcca gaattttata ggcggtttgg ctgttgagtg aaaaagcctg
    11761 ctcgtaggga gatttcaact ggttctcaaa tatttcaagg ttaattttct caatttcgac
    11821 gggcttgttc acatccagtc ccatctgaag tttgagcagg ttcttctgaa ccccgatggc
    11881 gctctgcatc gcggttctct gcgtcatcag gttggttttg gttactttga gcctgtcaag
    11941 gtcaaccttt ttaacaagcc cgttcgcata actcacctcc atggttgtca gcattgagtc
    12001 gataatattg atactcctgc caaacatctc cacggcataa ccggttacct gcacggcata
    12061 gaacagatta gcggtctgcg cgattacatc ttcctctttg gcgtcaacag ccagtgcaga
    12121 taagctttca gccgttttgg caatatccag ggcattaaaa agtgagaaat tcagaatttg
    12181 ctggttcagg ccggcgccta tggcagtgga ataatttaag cccatttcga tggtcatgta
    12241 atccgatgca ttggggtcca gcatactcgg cgggaggaat tccttcataa aattcggcat
    12301 gataaacttt gccttctgga agttgtcgtt caggcttccc gatccgctga tttggggaag
    12361 cagggcgccc gtcacttcgc ggcgcgcctg ggcgctcttt tcaacatcca ggtgcgtctt
    12421 gcggatgttg ttgttgtttt ccagggcata cctgatgcag ctttggaggt tgtatgcctt
    12481 ttgtcccata aggttcgagc aggcaaacaa caggaagcac aacattagtt tctttctgtt
    12541 tcccatatac atttaaagaa ttaagatcct attaacgact tgtcgaattc agaagtttcc
    12601 gcccctgttc ggttactatc gcctgcaggt ggtaatcaag gatactgttg aaataatctc
    12661 ccgccagaaa agccgaactg ttcgctaccc ggcttataaa aaagctgacc agcgaaaagg
    12721 taaaatactg gcttatcaat tcaacattga ggtcattcct gaagagttgt tcttcaatgc
    12781 ctttcaacag attcagttta accccttcaa gaaaaaatgc aaagccggta gtatgctcag
    12841 tgttggcgat ctcagggtac atctttttta tatcggaaag aaataccgga ctcagcgtcc
    12901 tgaaaaaacc ggctatttca tcaaccccgc atttcaaccc gtcaacggca ttaacatcag
    12961 cagaggtcat gatgctcatt ctctgcttta aatccagaac aaaagtgtga atgcattcgc
    13021 tcagcaactc ctctttcgag tcaaaatggt tgtaaagcgt ttttttggta accccgattt
    13081 tttcggaaat ttcgtccagc gacagtcgga gtccatcctg cttgaatgtt tccagtgcct
    13141 tttcggcata tttggctttt ctgtttcctg tcattgatgt atttcgtttg atttcatttt
    13201 aatagctcag accagagccc tttctcaatc tgatattatc agccatttcg atattcatcg
    13261 gggaggcaaa aatacacttt ttatatttta atagtgtaaa taatgaattt ttaatcagaa
    13321 ttttttacaa cactatcctc actatccttt cggaatctgt atatacagag ctccggattg
    13381 ataatcagaa ggattcagca cccgccagaa cggttattcc catatttcct gaaaacagag
    13441 ttccttgaat attactgctt tgtatcaaaa accatctctt catatgtgta cacccgggtc
    13501 agcgactacc ctgaactccg gttttcatat catcctttac ctcagccaac ggttcggaat
    13561 cacggagctg tttttacaaa tttctgctgc cagactgatc catctgtctg taacagcaac
    13621 acataagcac cggttgccag attactgacc gggacagcaa tgcaatgatt ctggcctgcc
    13681 ggattattca cctccgcaac cagccgtccg agggcatcaa agattttcag attactgata
    13741 tttatatcct tttctgttct aatgtacagg acatcgtcgg caggattcgg aaaaacggac
    13801 aaaaatccgg acgaggcagc ttcttttatc cctccgcctg ccgatttgct gatgtcaata
    13861 tccaataaat aggaacccgt ttctcctgca aaatagggaa ccacctggaa gtaaacggtt
    13921 cctccgtctg tcacattaat gctccccgac atcacatcgt caaaagcgtc ggaccatgtg
    13981 ctccctgcca aatataacca tactacgtca caggaataaa tatagccgtt attgctgcta
    14041 tagctatcct gagccctggc cgtgaccgaa taatcaaatc ccgaaggaag cacgatttta
    14101 taataatcac ggtcggatcc tttgtggata ttcgaccccg tcgtagttgc cgttatatgc
    14161 aacacatggt atgcattcag ggtcaaagca taagcggcag ccagggaatc gttattttca
    14221 taactatccg gaattatacc cggctcctga acgatgaccg tgatcggatt agggtgatag
    14281 gttgagcctg tcagttgcca gtttcctccc tcctctttgt gagtaagggc caggaggtat
    14341 gtcccgggtg aaacgctcac accgtcggta gaaaattgca ggtcatcgta aaaatagccg
    14401 ggttccagcg aggcgccggt taaggtggcg atattttccg cgaaacttcc gacgctgtca
    14461 gcgttgtaca aagaaacatc aaacatcccg ttaaaagtgg cggtaccgta attcgcaaca
    14521 tcaagcgtca cggtaaaagg ctgttcctga atgatattgt cgccgcaact aaggaccatt
    14581 tcctggtaca attcgatatc gttcgaatta ttcacggcaa atgagatcat gttggaatat
    14641 gaaccatcgt caaccatgat ccagtctccg ccggcaggcc tgaaaaagat gccgatataa
    14701 taggcccccg gcagcagcga caatgaaccg gagctggtaa aatcaaggcc attggtataa
    14761 tgcgaattcc ccggcaaact acatcctgac agaatttcga caaatgaaac gaagtctaca
    14821 ttttcatcga aaacggcagc acaaaaatct ccttcaaatg tactggtgct ataattagca
    14881 atatccgtat gtatggtaaa gctctggttg tatccaaccg gatttggact cgctataaca
    14941 ttgtcataca aggagatatt gtatgttgtg ccgcctgtaa ggggtttgat tccaataacg
    15001 gcctgctgac cgctgttata ggttcccata ccggcgccgg gattcaatgc atcgagcagg
    15061 aaataaccgt cggctatacc gctccagccc cagtttatat ggaaataacc gtttacgtca
    15121 tatccgtcgc aaacgaaagt atgtcccccg gctccgaacc cggcatattg aaccggccgt
    15181 ccctggtcca gatcattctt tatcaattgc ttccattcat tatccgaata atttgccctc
    15241 ttcagcccct gcaaggtcga cgggtcgtat ccgaaatatg ttttgtaggc atattcacaa
    15301 cattgttccg gggttggtga agccgcaatg atgacgtagg acccgctgcc atcaacgccg
    15361 taatccatct caacggctac cccgcaatga tacatgagcg tggctatcgc cagattgggc
    15421 cccgtcaggc tgttcggcat ggcgccccaa tcgtaagtgg ttgcagcgaa atttgccgat
    15481 aaagtgccgt aagtaggatg attataggaa tggaaacctg ttccctgtgc cgggtaattc
    15541 caatatttca tgatctggcc atggccgtag ccgggcaacc agtaaccgtc agtgcattat
    15601 aggtatcatc ataaggacaa agatcattat agtaagggct ttggtcccac gttgtcgtca
    15661 gcaggggagt gacggcggta atattccggt tctcattcag aggctgaccg gtttttagcc
    15721 gctgccactc ggatcggatc tccccagttg catccatctt ttttgttatg atttccctga
    15781 gctggttttt gtaaccttcg agccatttcc tgaagtttgg cggcagtttt gattcatcaa
    15841 agctcccgga ggcagagtag cccaaaacag ggattgcatt atcatcaccc gacacgatga
    15901 caaagccatg atgattcact tcattaaaaa cataaaacaa aggaaccgag tcgggagtat
    15961 tcaaatgtgt attgaattta taaacgcttg tgccggcaag ttccagataa atgccacctt
    16021 gctccttcac aacctgttga taaaggtttt ttgcaacaag ccgggccgtt tcagtgtcaa
    16081 cttcttttgc atcggaaagt ttcagcattc cggtacagac tactataagg agtaaaaact
    16141 ttttcatggg gttccgggat taatgggttt atacccggtc catataagga ttcccgaaag
    16201 ctgcaaaagg gagaatacca gttaacaagc agtattgtat tatttacaca aatggaagcc
    16261 ttattaaccg gaaacagaat atttaaaatt acaacatatc cgaattaaac acaaatgaat
    16321 tatttaagac acggattaaa gcccaatgtt tcggatgcat tgttatacat ggattgaggg
    16381 gcctttttgc aggaagataa ttacagaccg attatttacg cttatgtcat ccaccggaat
    16441 acttcactgc cccaactttt acaaaatcaa attgacagct aacggatatg gattaaaagg
    16501 atatgattta tcagcctgag ggtccatact attatgatga caaattacgg ggggctgggt
    16561 cccctaatct tgtatacaga ttacaaaata tttgatagaa ccggcaagcg aaaggcctgc
    16621 acaaaaaaag caccgcaaaa cggtgctttt tttgtgcccg gaacaggact cgaacctgca
    16681 cagccgtaag gccactggtc cctgaaacca gcgtgtctac caatttcacc atccgggcgg
    16741 atggattttg tttttgtgcc caggacaaga ctcgaacttg cacatccnnn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnntcc ttaaggacac cagcccctca agctggcgtg
    16921 tctaccaatt tcaccacctg ggcaaagcgg gtgcaaagat actatttttt tgaaatgagc
    16981 aaactattga tttcattttc ggaaaatttc ctccggatta agatccctgc tgccaattgg
    17041 aaactttcat agccgataat cgactatatt tgtgtttcaa accaaggaag tgaaaaatat
    17101 gttcaccaaa gacgacctaa agcagttaca gtcgcacggc attgatccta aaaccgccga
    17161 gttgcagatc aataatttcg tcaaaggatt cccatacatc aacctcgacc gccccgccat
    17221 tgtcggcgac ggcataaagg ctttcaacgc aaaagaggcc aaaaaactct cctatttcta
    17281 tgattccaat tcaaaaaaat acgaaatcct gaagtttgtt cccgcatccg gcgccgccag
    17341 ccgtatgttc aaacacttgt ttgagttttc cgaaacatac aacgggactg agaaagacat
    17401 caccggacta ttaaacgata accaattact gcctgtaaaa caatttttcg accacatcaa
    17461 agaatttgca tttgccaaag ccctgtcaaa agcgatgcgc aaggatgact ttgacctgga
    17521 cgactgcctt gcccggaacg attacaaacc gattatcgat tacctgttga ctgaaaaagg
    17581 tcttggttat gccaatctgc cgaagggcgt cctgctgttc cataattacc cggatgaacc
    17641 gcgtatggcc attgaagaac accttgttga aggcgccaat tattgcaagg ataaggaaaa
    17701 acgcgccggt atccacttca ccgtttcgcc ggaacatgcc gaccggttcc tggacgaaat
    17761 taaccgggta aaggaaaaat atgaagagct ttacgatgta acctatgaac tgactttctc
    17821 tcttcagaaa acttcgaccg acaccattgc cgtcgatctt aacaacaagc ctttccgcga
    17881 cgctaacgga aaactcgtct tcaggcccgg cggacacggg gctttgatcg aaaacctgaa
    17941 cgatcgcgaa agcgagatcg ttttcatcaa aaacattgat aatgttgttc ccaatcatcc
    18001 aaaagaggac acattccttt acaaaaaagt attgggcgga tatctcttca acctccagga
    18061 ttctattttc gaattcatcg aaatactcga agacaagccc gacgatgaag acatccgcac
    18121 catcctgagg ttcatcaaaa caaaactagg tttcgccatt gatcccgatt tttcagcatg
    18181 gcgcaaggaa gacaaggttg agtatctgca ccggaaactg aaccgcccga tccgtatttg
    18241 cggcatggtg aaaaacgagg gcgaacccgg cggcggtcct ttctgggtga aagaagcgaa
    18301 tggcgaaatt tcccttcaaa tcgttgaatc ttcgcaaatc gacctcaatg acgaaaaaca
    18361 gaaagccata ctgaaccaat ccacacattt taaccccgta gatctcgtct gcggcgtccg
    18421 cgactataaa gaacgcaatt ttgaccttcg caactacgtg aatccatcta ccggctttat
    18481 ttccataaag tcgaaagacg gcaaatcgct gaaagcacag gaactgccgg gtttatggaa
    18541 tggcgccatg tcggactgga ttaccgtatt cgtcgaagtg cccgtcagta cctttaaccc
    18601 ggtaaaaaca atcaacgatt tgctgaggcc ggaacatcag taaaagaatg aattatccga
    18661 aaacctgcct gccctgatga ttagtccgga cagaaccggc aggtccggca atccggggaa
    18721 aagcggggtc atccttggtt catatctggt tatcaggaaa atgccagaaa aattcccccg
    18781 atgtataaat aaactaccgc tttgtacaat ctgcccctcc tggtattggc tgtttgttta
    18841 tcgcgttaat tttgataaat tcgcaacgtt aataacgccg gctggctaaa ccctttcact
    18901 aattacagta cttatgctga atggcttccg aaatatcctc tgcatccttg ctgtatcagc
    18961 cattttttct cctggtttat atgctcaacc aggaatgagc catagtcagc ggactgctta
    19021 tatcaaagag ctggacgggg aaaggaagca aaaggatgaa gaaatgcgca cggcagagaa
    19081 atcgcctttg cctgcggatg caaaagccgg attccggggg ctgaattatt tcggaggaag
    19141 tccggaatat caggttaaag ctatcctgaa acgtttcgat gatcctataa cgttcagcat
    19201 gaaaacgacg acggatcgcc tgcctgaata ccggcttttt ggcgaagctt cttttacact
    19261 taaaggggag ccctgggtac ttgaagttta tcagaatgtt gagctttcga aaaaacccgg
    19321 ctatgccgat taccttttca tcccgtttaa cgacaggacc aacggaagag caacatacgg
    19381 cggaggccgt tttatagatg cccgcatccc ggaaggagac acgttgatca ttgattttaa
    19441 ccgcgcttat aacccgtatt gcgcttacaa tcataagtac tcctgccctg ttccgcccga
    19501 taagaacaac ctggaaacaa aaataaaggc aggcgagaag aaatttcact gattataaag
    19561 taacccatta ccttttaaga acttggcatc aaaaagcagt tcagttgagt ttgaaaccta
    19621 ccggtcgtta aggcatttca gaaatttcct gcgaaggccg aaatatcctc atgcgacggt
    19681 taaccggatt gttccgcacg tagcctggat catcctcttt tggcttgccg catacctata
    19741 caaagaaaga attttcgccg attccgggtt ttatttcagc cagtttgtca ataacaagac
    19801 tttctggata gaatgtcagc gcattgtact ggccttttcg cagatcatcc cgctggcagg
    19861 cgtatgggcc ggcatcccgc tgcaatatgt gctcctgctt tactcgctga gccatgtggc
    19921 gttcttttat ttcctgttca tcttcgttta ctacggaatg cgcgaccgcc ggtcggggtt
    19981 gctcatcatt ctttcccaaa ccgtcggaat tatgtacagc ttcttcactc ccatgttcga
    20041 gctgtactat ggtgttccgt tgctcataac cttttatgcc ttctggcggc tcgagctcga
    20101 caggcctttc atcaccttaa ttctcatcat ccttgaaatc ctgcttctcc tgagccatcc
    20161 tgtagctttc attctttttg tatttctatt gctctacgac atccctgaaa gaagggaaag
    20221 caggaagccg ctttacctgt atatcctggt agccctggtt tacgtgggtg ttgtcatcta
    20281 caagggattt accatgtgtg aatatgaaac cggcaaactg gcctggcaat tcaattttac
    20341 cgaaaacaag acttatctcc agttgcttga tccggcctat tataacacca tggccctgtt
    20401 ctttttaaaa tattactggg aagtgattgc agcgcttctg gtagtgacca taacgctgct
    20461 aaaacagcgg caatggtttc gtttgctgtt aacatatgga ttcctggctt cgtacctgtt
    20521 cctggtaaat tcggcttatg ccgtatccca gtcacggtat atggaacagg tcatgtttcc
    20581 cgtggttccg attgttttca ttccgctgat ttacagtttc ccgaaagagc cgaggcctgg
    20641 cataaaaaac atctctgttt tcctgatcgg cgccctgata gcatacaggc tcttcataat
    20701 ctacgaaggc agccatattt ttgttcaacg caccaggcag atggaaagcc ttatttcatc
    20761 agccaggcaa atgggcggca gtaaatttta tgtttacccg caaaatatcg accgcggcta
    20821 tacccagttc aattggtcat atccgctcga aacactgata ctcagctcta tacgcggaaa
    20881 caattatagc gtgactattg cacctttcga tgacctggag ttccaagaca atatccggaa
    20941 aatgggcgcc aacgatttcc tgttccgtcg ctgggaaatc aaggacaagt cctggttaaa
    21001 cagccggtac tttcacctgg accccgggct gtaccgcacc atgaacgatc agtcgcccaa
    21061 cgacgacctg gagtatatgg ccagaaactt ccgtatcgcg atcgaaacaa ggaattatta
    21121 caattcactg gataccgtct ggatgaaagt ccacattaca aacacaggaa ccgatatcct
    21181 gcataccggt tcgaaaaata aaatattcct ctcctacttc tggaaaaaag acggggatgt
    21241 actcaactgg accaacatcc agacaccggt agaatctgat atcgtccatt cccttaccca
    21301 ggatatgctc gtagctgttc cttcggttaa agggaaactg caactgcagg tcgatatcat
    21361 ttctgttgac aaacaatggt tcggcctgaa tgcaacaaaa gacattctgg tctattaatt
    21421 gataatgaac aacgttgctg aagataaacc catgaatgct aagcgaatac tcatcctcac
    21481 aattctgctt ctcactgccg gcattataca cgctgcgccc aaaggataca ggatcatcgt
    21541 gaaactgcat aacagcaccg atacagtgct gcttatggcc cactatttcg gcgacaagca
    21601 atacctcgac gataccgctt ttgtcggcaa acagggcaca tatatctttg aaggagatga
    21661 gccgctgaaa gaagggatgt atattgttgc cggtcagaat aaaagccgtt atttcgattt
    21721 cttcatttca ggcccgcagc agtttgagtt ggactgcgat cccaacgatg tcaccggtac
    21781 aatgaaagtg aaaggctcgg aggaaaacaa agttttcaat gagtatatcc gataccttgc
    21841 cgaaaagcag aaagaaatcg aaccgctgaa caggcaacta aaaagcctga agcccggcag
    21901 cgattcggca gttttaatca acaaacagat tgaagaaacc gataaagagg tcaaggctta
    21961 catgagcaac ctgttctcga aatacaccgg cctcctttct gtaaaatttg taaaagccaa
    22021 taacgaaccg gacataactc ctttcctgaa agatcagaat ggcaaagttg attcaacccg
    22081 gctgtacccg gtgtacaaac agcacttctt cgataatttt acgttcggcg atcctcgttt
    22141 gatatataca cccgttttca ctgcccgcat cgaccgctat ctgcaatccc tggtcgtacc
    22201 gacggctgat tcgctgcaaa cagcaatcga tcatcttctg tcattggctt ccgccgacaa
    22261 agagacacaa aagtacctgg catggtatct ggcagtgaaa tacgaaactt ctgagatcat
    22321 ggggcacgat gcgctgtttg tttacatcgt gaaaaaatat cttgaacccg ggaaaataga
    22381 atggtattac ccggaagtga aagaccgggt aacaaaacgt gccggcacgc tcgaaccgct
    22441 tcttcttggc aaacaagccc cggatctgat tctgctcgac accttaaatc ttccgcattc
    22501 cctgtattcg gtaaaagcgc cctataccct tctgtttttc tgggaaagca cctgcgggca
    22561 ttgcaaacag gaaatgccga atgtgcttaa gttttatgaa gaatttcaca aggactacgg
    22621 tcttgaaata tatggcgtga gcggcgatac ttccatggta aagtggaaag aatacatccg
    22681 cagcaacaac atgccatgga tcagcgtaaa cggatacctg agcctgaaag gcaattacca
    22741 tgacatttat gacattcaca gcacgccagt catgtacctg ctcgacgaaa acaagaagat
    22801 actggccaag tttctccttt cggaacaaat gggcgacctg atcaaaaaac gggaaggtgc
    22861 aaggaagaaa gatgaataaa cagaaatagt cctttatata cacctttcga ggcattatca
    22921 taaattaaaa aagccatccg ctgtcagcag atggcttttt taattttaga actaacaggc
    22981 tatttcacaa gctccacgat agctttgaag gcttcggggt tgttcatggc caggtcggca
    23041 agaaccttac ggtcgagctc aatgttcttt tcatgaagtt tgcccatgaa taccgaatac
    23101 gacatgccgt attcccttac gccggcattg atacgggtaa tccacagggc gcggaaattg
    23161 cgctttttgg tgcgcctgtc gcggtatgca tactggccgc ccttttcgta tgcatttttg
    23221 gcaaccgtcc agacattttt acgccttcca aactggccct taacggcttt caggactttc
    23281 ttcctgcggg ctcttgaggc gactgcattt actgatcttg gcattttttt cgattttttt
    23341 tacttcagcg tctctgcttt cgacagagaa ctttcggggc tgaaagtaag gttaaacatt
    23401 tgttttgttt agcactggag catgtccttt acggctttca catcggcctt gtcaacgata
    23461 gccacataac cgaggttacg tttgcgtttt gtcgatttct tggtaaggat gtggctcttg
    23521 taagcatgtc tccgtttcag cttgcccgtg ccggttgtgg tgaacctttt tttggcactg
    23581 gctttactct tcatttttgg catgatacta gggtttatta attatttaag ggaattctct
    23641 tttagtagtt attcgttgtt cgtgattcgt tgttcgttgt ctgcttgtca tgactgtacc
    23701 ctggataatt ccggggatgg caggaatcat tattttctgg aaaacaaatc accgtttccc
    23761 gttcactatc atccgattac tttttcggcg aaatgatcat aatcatccgc tttccttcca
    23821 gcatggggag tttttcaagt tttccgtact ccatcagttc ctgggcaaat ttcagcatga
    23881 tgatttcacc ctgctcttta taaacgatgg accttcccct gaaaaaaact tcaactttaa
    23941 ccttacaccc ttcctgtaaa aatttctggg cgtgtttcag cttgaagtta aaatcatggt
    24001 catcggtctg cggaccgaga cggatttctt taacaacaat tttagcggtc ttggccttaa
    24061 tttctttttg tttctttttc agttcataaa gaaacttctt gtagtcaatc accttgcata
    24121 ccggagggtt tgccgtgggt gagatttcaa caaggtccat ttcgaggcct tcggctatca
    24181 tcagggcttc cttgatgttg tatatgcccg gtgttatgtt ttcgcccacc acccttacaa
    24241 caggtgcttt aatgcgttcg ttgatctgat gcagttcctc cttttgtttg cgaggatagc
    24301 gccgatccat cgggttgtta ccaattgttg ctatacggtt actcctttct gttggtttgt
    24361 actcatattg gtttatggtt aatcgtaaat ggaaattcgg agttcagaat tttaacttag
    24421 ataaaaatct gatttataca attttatatc catttacgat taatctctca gtcaagcatt
    24481 tgttttattt cctggttaat taaaacgata aattcatcga tgctcatcgt acccaagtcg
    24541 ccctggccat gtttgcgcac cgatacggta ccgttttgtt cttctttttc gccaacgatc
    24601 agcatatacg gaaccttcaa taattcggca tcccgtatct tcttgcctgt cttttcattg
    24661 cgttcatcaa cgagggcgcg aatttcggat ttttttagga ttcctgcaac tttatttgca
    24721 taatcgagat atttctcact tatcgtaaga atggtaacct ggtcgggagt gagccaaagc
    24781 gggaaatttc cgccgccatt ctccagcaaa acagccacga aacgctccat ggagccgaaa
    24841 ggcgcacggt ggatcatcac cggacggtgc ttctggttgt cgcttcccgt gtattccagt
    24901 tcaaaacgct ccggcagatt gtagtccacc tggatggtgc cgagttgcca cttgcggccg
    24961 agcgcgtctt tcaccataaa atcaagcttg ggcccgtaaa acgctgcctc cccgatctcc
    25021 acaatggttt tcagcccttt ttctttcgcc gcttcgatga tggcgctttc ggctttctcc
    25081 cagttctcat cggtgccgat atatttttcc ttgttgttgg gatcgcggag cgaaacctgg
    25141 gcgatatatt ccttgaaatc aaggaccctg aagatatgca atacgatgtc gatcaccccc
    25201 ttgaattcat ccagcaactg gtcgggacga cagaagaggt gggcatcgtc ctgcgtgaac
    25261 ccgcgaaccc ttgtcaggcc gtgcagttca ccgctttgct catagcgata aaccgttccg
    25321 aattcagcaa aacgaagggg cagatcttta tacgacctgg gcttgctgtt atatatttcg
    25381 caatggtgcg gacagttcat cggcttcagc atgaactgtt cgccttcgat gggcgtattg
    25441 atgggctgga aggagtcctt cccgtatttc tgaaaatgtc cggaagtctt atacaattca
    25501 acgttcccga tatgcggtgt aattaccggc tggtagcctg cttcctgctg aacgtttttc
    25561 agaaaacgtt cgaggcgttc acgcaacatg gcgcctttgg gaagccacaa cggcaacccc
    25621 tgccctacct tctgcgaaaa cgtgaaaagc tccagctcac gccccagttt acgatggtcg
    25681 cgttttttag cctcttcgat catcaccagg tactcgtcaa gttctttctg cttggggaaa
    25741 gtgatgccgt aaacacgtgt cagcatcttc cgtttttcat cgccgcgcca gtaagcgccg
    25801 gcaatattca gcaacttcac cgccttgatg gaagaagtat caggcaaatg agggccgcgg
    25861 cacagatcag tgaaactccc ggtttcatag aacgtgatat gcccatcttc caggtcattg
    25921 atcaattcca gtttatattc gtcattcttt ttggtgaagt aatccaatgc ttcagctttt
    25981 gacacctcgc gacgcctgaa tgtttgtttt tcacgcgcca gttccagcat tttggcttcg
    26041 atggccggca ggtcttcggg agtaagctgg cgttcgccga aatccatgtc gtagtaaaag
    26101 ccgttttcaa tatcgggccc aatcccgaac ttcacacccg gataaagctg ttcgatggct
    26161 tcggccatga ggtgcgccga cgaatgccac agggttgctt ttccttccgt atcattccag
    26221 gtaagcagtt gcagccttgc atcggaagta agcggacggg tggcatccca cacttcaccg
    26281 tttactttgg cggccagcac gttacgggca agcccctcgc tgatgcttaa tgcaacttcc
    26341 agcgatgtgg tgcctttggg gtactgccta acggaattat cgggtaatgt gacgtttatc
    26401 atggtattca cggcaaaatt ttaagggtac aaaggtagga aaattaacaa tttcccgaaa
    26461 agaaatgttt acagagcgac agaaagaaaa gaattgagag acagcctgtt tatctgtatc
    26521 tacttaagtt cagaaaaaaa tgatcacata ttacaaatgc caatatatat tcattatatt
    26581 tgaaagggca atatggtata ttaccatgaa atagtaagat taaaagatca ccgttccaat
    26641 tcacatatta tatcactaaa atagttatga aaatgaaaac aaaattcact ttcgaacatt
    26701 cagggctgct attcgctttg ctgcctctga caggtatcct cttaaccctt aatttggcat
    26761 gtaaaaaaaa tacattatct gagctggcac caaataatcc tcttaattat actctaaaag
    26821 atgaagaact aatcgatagc gtttatgctt ctgacggaag attagttttt acttcgctgg
    26881 ctgaatacaa tttatttctc aatgacattg gaaccttaaa tgattcatca ttgagagtta
    26941 ttgagggcga atataactat acctcattac gtgaatctgt taattatgat gatagctttt
    27001 taccagagga agacaaagta ctaggaacca tttttaattc acacagcata tttcaggttg
    27061 aacattgggt tttaaagttg gatggtgata cggtgtatgc tgtttatgat tatctatata
    27121 atgaagactc cgaaggttgt tggactgatg aatctgtgat taaagtttct gagaaccaag
    27181 atgtttggtt cgaaaccggc ctcgacaacg aggactgcaa tgaaactaac agaagcgatc
    27241 agacagtaaa agatactttt tatctgataa cagcaactgc aaataattca aattcatacc
    27301 aatatggttt tgaattaaaa ctgaaatata atacttcatg ttttggactt tcacataagt
    27361 atatcatcta tggaacattt ggttgttatt ataatggagg atttcaatca ttggcatctg
    27421 gtggaaccta tacactattg cctgcatcac ctttttcaat gaaacttaat gaatattatg
    27481 ccacattgga tccaagatgt aagagttgca aaacatatca aaaaaccaat gagttgttta
    27541 tttataggtt tgatcagact ctctattctt caatgagagg attacaatac cataaaggaa
    27601 aagtcaaatg cgatctaaca attggtagta atactaaatc cacgccattt ttaactatgg
    27661 aatcttggca tccaagttca tgtgattgtc ctacctggta attttggaag cccggttgaa
    27721 aatatgacta tttttaacgg ggcttcctta tatgttttga tacatgaaga actcattgat
    27781 gttatttttc ttggtcatta catcaaggtt atttgctcag gatgtattgc atag
//
LOCUS       DAOPYV010000040        27284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_1733_length_27284_cov_11.102758, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000040 DAOPYV010000000
VERSION     DAOPYV010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27284
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1733_length_27284_cov_11.102758"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="PKI35_06795"
     CDS             complement(<1..1519)
                     /locus_tag="PKI35_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44128.1"
                     /translation="MKNFLRFIRYICPSVSKHASTALMALLLIPFLASPLRSFGEGTK
                     ELLPSDPAIVPTTRGCLYLSSGSTYLPWALHSNTDDNLRLKFSVSSNWANEVVFIGLN
                     SVNNISSFYIRYPDGTYSAAQTFPTGGPGYISNWNQAVSGPNVINPSGYEPIVFTPTM
                     EGDYSIDFEGNFSGSATIEYFDITVATSNGGGSYTPIPGRVWSKVWQIYAMHSNQFDN
                     FRGIFYVYSDDHIVTKFNANGMRVGACDIYCNEWGTSTTGDWETRRKSIEGKAGVVPQ
                     YKMFLNEPDIALFPTGILGQIISAQQTSSSCDGSVIFEIVVDKPGKVTLSIDLPPAGL
                     GDPNDVYLNQNVVAGSNLITWNGNNGSGQPVNNGASISMSIDYLNSLTNLPLYDVEGN
                     EDGIKVDIHKPLPDPLPPDGTKLRIYWDDTNPGLGGIDNSVTGCMYDDEPPVTGCHDW
                     SFSSSGGLGDENTINSWWYYLSQNNVTISVSIIRTPATPTVSPTGPSPVCQGQTGA"
     gene            complement(1709..4450)
                     /locus_tag="PKI35_06800"
     CDS             complement(1709..4450)
                     /locus_tag="PKI35_06800"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNX44129.1"
                     /translation="MLLRFHIEYNTSPGQQLIVAGSVPELGNGCPDKAPFMTLHNAKN
                     GLWTYCLELNEDKDFRYRYFVKDGNSNTFLQEWGPDRVFHPEKRHDKTILLSDRWRAA
                     SDPEYALHSSAFLNAILKPGDPHKAPKIETGERKDSVILRFKPNVVRIKPGHRVGVSG
                     NAKALGNWDDRKAIPLGNPDHPEWCGEVIVKIAEFPVKYKYLFLDEEGKTVFWEKSAD
                     RVVALPKGDIPDIIEIGDEKFDFPQYPWKGAGVAIPVFALRRADGFGVGEFTDLKLLV
                     DWAVQTGIKLIQVLPVNDTVAKHTWQDSYPYAAISVYALHPIFINLLEIGTLEADISQ
                     QIIDAQGKYLNSLKRIDYEAVMALKSRFFKLIYDQKKTEFLTDPEFLAFFADNEYWLK
                     PYAAFSYLRDLFNTPDFSRWGEYSKPAEELLEHLTHENSPHYDDIAVHYFIQYHAHRQ
                     LLDAANYARSKGVVLKGDIPIGVYRNSVDAWLHPHLFNMDRQAGAPPDDFSAKGQNWR
                     FPTYNWDEMAKDNYAWWQQRLRQLSYYFDAFRIDHILGFFRIWEIPSCQAEGLMGYFN
                     PSIPYNLDELQHRGLGFDEKRLCRPYIREHFLYERFGDLTAYAKENFLTEFYPGCYDL
                     RPEVDTQRKVEEKLAIEPDMGPEERSRLVRLTQGLYSLISEVVFLEAPDSGGNAFFPR
                     NALHYTRSYQELDEHSKHLIDEIYLEYFYHRNEEFWRKKAMEKLPVLKKTTNMLLCGE
                     DLGMVPACVPDVMKELGILSLEVQRMPKDPKITFGHPADYPYLSVATPSSHDTSTIRG
                     WWEENPSMSQKFYNEILGKYGKAPFFCETDIVKQIIVQHLYSPSMWAIFPIQDLLGMD
                     EKLRLQHAPSERINQPGNPSHYWRYRMHIDLESLAEQTDFNEMVKEMIHSAGRLDIY"
     gene            4860..6146
                     /locus_tag="PKI35_06805"
     CDS             4860..6146
                     /locus_tag="PKI35_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNX44130.1"
                     /translation="MKHSITFLVEARKQQQPFAPINVDVNYTGLRIRYYTGYRLSTEN
                     WVYNGSWIMDNWDVNAHRVVSGYKAYDGKTQVMAIQVNRKLDKVNGNIADDLFNTDLP
                     PSKDAVLSYLDSIFKPYKKQKERAEKQKELSFWQMYERYCKEAKVSPGRRKQIKVTMN
                     HLKAFDPNLTFAAVTPEKLRKFERYLLSDDPPKSKNTVSGQIKKFRAFWNWAKLERSN
                     LHYPFEGYKIDAEEYGEPIFLTKDEIETLYRAKITDERLKRIRDIFLFQCYVGCRVGD
                     LVKLTTDNIINGSVQYIPRKTKDNKPVTVTIPLTKRAKEIISRYKVPGGMLLPFISPQ
                     KYNDAIRDLFKNNKVKLDRTVIRLNPLTREEEAVKLCDIASSHLCRRTFIGNLFGKVD
                     TSIIQSMTGHAKDSKAFGRYYSVTDELKQQAIKNLE"
     gene            6148..6921
                     /locus_tag="PKI35_06810"
     CDS             6148..6921
                     /locus_tag="PKI35_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44131.1"
                     /translation="MEKQEFLKKLSEVIDELPQLVDEVSRKTKEIVKYEFQQINKKIA
                     FSPSDKDDILKEYKKRVIGELNVNMINDAGELNVKWYKDFLIQKLNFCSKWEWDSSTP
                     ENRLPAPLHMILLPREHVVIEITKVIYNKELIDYCNYHLQHNEAGLGLAATKAKTECD
                     VIAKQLEGKGFIPRGDGAKFISIFFPDGRPFEKVTWLKSRKDCFSLMMEMNDPDIKPK
                     VINKYINQAGIDPKTKKVNTKPLRSQDKQNCTAFLRRLK"
     gene            7089..7427
                     /locus_tag="PKI35_06815"
     CDS             7089..7427
                     /locus_tag="PKI35_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3853 family protein"
                     /protein_id="HNX44132.1"
                     /translation="MPMVVLTSDELTHIIRGVISDEIGKRLDAKPPRPTIKGIHGLAE
                     ALNCSIAKAQKLKNSGQIPYFQNEKLLLFDFDQVMDALKGTDIHRRGRRPRHVEPERA
                     VEIRRVNERS"
     gene            7414..7977
                     /locus_tag="PKI35_06820"
     CDS             7414..7977
                     /locus_tag="PKI35_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44133.1"
                     /translation="MRGVDKNTEAPTGQDGGFNNALVGKRQRKDTAFQALSALYLKEN
                     IRKYPSLPYRCVPKWNDCTSNGLTRMIIDFLRLSGHQAERISNTGRMLDGRKIVVDCI
                     GNRRLIGSTAWIPGTGSRGTADISATINGRSAKIEVKCQATKDKQRPAQAEYQKQIEA
                     AGGTYYVAQTFAGFFNWYNKTFGGANQ"
     gene            7974..9047
                     /locus_tag="PKI35_06825"
     CDS             7974..9047
                     /locus_tag="PKI35_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44134.1"
                     /translation="MNEKLIPPPDLNLNGEPLPETATHTLKTFEPYRITDTTQIPAPV
                     PVIKISGETISTPEHITVISGAPKSGKTSFCSILIAGAISKTGEIFDTLDLVNVLPNR
                     NQLAVIHFETEQAPWKHQANQAGILKRAGLDHCPGYYLSYNIRQIPIEELNEFISGVC
                     QAAAAQFGGIFVVFVDGIADLIRDPNDQAESFDVVKFLEGIAQEYFCPVIVVLHTNPG
                     SDKERGHLGSQVQRKAESVLQVKKEGNVSYLDPKLLRHAGDDIPKVQFQYDRDKGYHV
                     QCNVDNPTDRRAIQKVEKLEALAGKVFSGQAAFKYSDAINVIIRHTGYGQTTAKRLFT
                     EMKACELIEQGNDNNWRKVNKPV"
     gene            9151..10278
                     /locus_tag="PKI35_06830"
     CDS             9151..10278
                     /locus_tag="PKI35_06830"
                     /inference="COORDINATES: protein motif:HMM:NF020379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BT4734/BF3469 family protein"
                     /protein_id="HNX44135.1"
                     /translation="MFSYFTNHSQNIPAGVITLPELHRQLIAPDREPFLRAANLVKHL
                     RAATDEAEAKAIKSQLPAICPGATLDTRATGATPEQQNISYSGFAQIDIDLHDNPNMT
                     DAAEVRDKLAQIPYIALTAISARGRGVWALIALKEPGKFTEYAEPITEFFKRARVTID
                     KSKSKRPTELRYFAPDPGAILKNEYQLFPLIRPQSKPKPAAGNRQHTGATFEQVQRWV
                     SETTGFNLVDGQKHYYLFWLAYGLRKNGMDPQSVYQLIYDNVLPESCVHSNCISGGIA
                     YANTKGIYTPQTAKTAPQITHPRVTKTTPASTREPVTPRQTKLITNCIKTVTGDIYRP
                     LPYGRYQRYTGDTPGEIIGFGDSLRLINSPSFDCLYTLKLN"
     gene            10281..10478
                     /locus_tag="PKI35_06835"
     CDS             10281..10478
                     /locus_tag="PKI35_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44136.1"
                     /translation="MRYFTKLTDLAKHYNVDESLLFKAYCKMTGNSDPENMSRLWQQT
                     NGEVLLAPCEVEKIINIMMYN"
     gene            10765..11217
                     /locus_tag="PKI35_06840"
     CDS             10765..11217
                     /locus_tag="PKI35_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44137.1"
                     /translation="MKKTLKTEKIQRGTYASSRDKSAKGLPVLLLSEIPEAPAHLTAS
                     AAEIFRATCQSLKNARKLTADTLNLIADYSYFSDLAAVWQQRSEKIDQLMGESFDPVM
                     MRKFVAANRLSRQNSSHACKLASQLGILPGDRNPERDQDSSFEREFLQ"
     gene            11321..11551
                     /locus_tag="PKI35_06845"
     CDS             11321..11551
                     /locus_tag="PKI35_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44138.1"
                     /translation="MKTLNEAREQLEKELSNLERQQPGADSMQLIGAVRQLLGLANMG
                     LLPDVRARLTELKMSSGPDGRTNRIENNYTKR"
     gene            11556..12188
                     /locus_tag="PKI35_06850"
     CDS             11556..12188
                     /locus_tag="PKI35_06850"
                     /inference="COORDINATES: protein motif:HMM:NF016471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HNX44139.1"
                     /translation="MNYKEQRNTDRELRYIDGSRHVVGLAVVFNKRAPIGDFFEQVAP
                     EAIDDDVLNQDVRCLFNHDSNFVLGRTKAGTLKLTKTAEGLEYSLDMPVTTFGNEVLT
                     AIKLGNVSGSSFGFTVKKDEWKELPGGIYLRTILKIGKLFDVSPVTFPAYADTTVGIR
                     SLEAWKRSHGGKSIQDYEQELKKMELEMNRPQPHRTTLAEAERQLKALRP"
     gene            12337..12846
                     /locus_tag="PKI35_06855"
     CDS             12337..12846
                     /locus_tag="PKI35_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44140.1"
                     /translation="MLRSRGGVDFYADSYADFAANPITRCPDDVRHADFKAGRVITDR
                     IEAFHHNAAVLIQKVKHRVNNLRQTEEDAFTLENLDNRTFKDFAQYRDYCSKVQAGQI
                     ERPPREKKPAPDPEAVKRRKFYQEVIQRQINAGQSLETAYHAQNIQVPGIFGSFKEYE
                     TYHKHPEVL"
     gene            12843..13127
                     /locus_tag="PKI35_06860"
     CDS             12843..13127
                     /locus_tag="PKI35_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44141.1"
                     /translation="MKATEKEKQEFYSLVRTVWRNPRFSGQPAMFPFHEAVKLWRTNH
                     TCLPFETVEEFRAYGGNYGQRSATPIQQEQRQYLNQRDKIRVLKNTHYRQ"
     gene            complement(13370..13443)
                     /locus_tag="PKI35_06865"
     tRNA            complement(13370..13443)
                     /locus_tag="PKI35_06865"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13407..13409),aa:Arg,seq:ccg)
     gene            13616..14695
                     /gene="fbaA"
                     /locus_tag="PKI35_06870"
     CDS             13616..14695
                     /gene="fbaA"
                     /locus_tag="PKI35_06870"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNX44142.1"
                     /translation="MTDKVFDKIAPGVVTGDDVQEVFRIARRNHFAIPAVNVVGTDSI
                     NSVLESAREVNSPVIIQFSNGGAHFVAGKFLNNDGEQAAITGAISGAKHIHMVAKMYG
                     VPVIIHTDHAAKKLLPWIDGLLDAGEKHFAECGVPLFSSHMLDLSEEPLKENIEICKK
                     YLTRMSKIGMTLEIELGVTGGEEDGVDNTGVDSARLYTQPEDVAYAYEELLKISNRFT
                     IAASFGNVHGVYKPGNVKLQPVILHNSQVYIKNKFNTEENPVNFVFHGGSGSSREEIR
                     EAISYGVVKMNIDTDTQWATWKGVLDFYKKNEGYLQGQIGNPEGEDKPNKKYYDPRKW
                     LHESQKSMVLRIKEAFEDLNCLNRN"
     gene            complement(14744..15595)
                     /locus_tag="PKI35_06875"
     CDS             complement(14744..15595)
                     /locus_tag="PKI35_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNX44143.1"
                     /translation="MSNLIAAVDFSDCSINALEHAVSIANRGLLDLHMVWVNNPSVTK
                     TTIYSDTSSDLIDQIKGQFAKLIEKYKPRLPESEIDYVIREGKVYREIIDEAKEMNSI
                     CIVMGTHGASGFEQFWIGSNANRLISVSHCPVITLRSGISIKHDLERIVMPLDSTLDT
                     RQKVPFTAYLAKLFEAEIHILKLYSNKVKAVQHTIDEYAEQVIQYLDEEEISYVLDSV
                     QADNLTVATIDYAEKVKANLISIMTEQETKMYNLLIGPYAQQMVNTSPFPVLSINPKE
                     TLIMASH"
     gene            complement(15753..16175)
                     /locus_tag="PKI35_06880"
     CDS             complement(15753..16175)
                     /locus_tag="PKI35_06880"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNX44144.1"
                     /translation="MKKSVLILFAILLAGAQFSRAQEQQNAPGKVTIYQDDKMVKLAD
                     LYKEVNQRDPSFDGFRVQIFFDSGNNSKNRATEALETFQEKYPEVKAYLSFKQPYYRI
                     RIGNFRTRSEAIGFQKRIQADYPNAFTVKDRILFSEQD"
     gene            complement(16181..17011)
                     /locus_tag="PKI35_06885"
     CDS             complement(16181..17011)
                     /locus_tag="PKI35_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNX44145.1"
                     /translation="MKEILVAIDFSKGSLHALEYAVALANTCNSNITMVWVDSPAMLM
                     QAESSEMRDEARTNMEEILHTEKYKLLGGKLSYKLRKGKVYQELAGQARLSGADLVIA
                     GTHGITGFEEFWIGSNALRLVTSVPCPTITVRTSYDINRPIKKIVMPVDHTALTLHKV
                     PFTASFAHSFGAEVNIIALNSSKLKTVQRVIDGAVVKVEKYFHDHKIPFITEKQNTDN
                     IATSIIEHSRLIDADLISIMTDGDVEASGVLLDKFSQQLINYSEIPVLSNHPVERFLL
                     "
     gene            17155..18183
                     /gene="ruvB"
                     /locus_tag="PKI35_06890"
     CDS             17155..18183
                     /gene="ruvB"
                     /locus_tag="PKI35_06890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNX44146.1"
                     /translation="MSLNKNLDATGEYMTPAEREIERALRPPDFGSFSGQAGVVGNLR
                     IFVAAAKQRNEPLDHVLLHGPPGLGKTTLSYIIANELGVGIKMTSGPVLDKPGDLAGL
                     LTGLETNDVLFIDEIHRLSPVVEEYLYSAMEDFRIDIMIESGPNARSVQIKLNPFTLI
                     GATTRSGLLTAPLRSRFGINSRLQYYDAETLKHIIKRSAGILGVPIRDDAAHELARRS
                     RGTPRIANLLLRRVRDFAQIKGNGTVDLAISQYALEALLVDKNGLDEMDNRILASIIE
                     KFKGGPVGLTTIATAVGEDPGTIEEVYEPFLIQEGFLMRTPRGREATEKAYLHTGKIR
                     TGINGSLF"
     gene            18225..19181
                     /gene="queG"
                     /locus_tag="PKI35_06895"
     CDS             18225..19181
                     /gene="queG"
                     /locus_tag="PKI35_06895"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNX44147.1"
                     /translation="MIPHELSVRIKEKAAALGFSGCGIARAEHLAGDEERLKRWLDAG
                     RQAGMQYMERNFEKRTDPRLLAEGTKTVISLLYNYFPGKETNCENGFRLAKYAYGVDY
                     HELIREKLDILCKVMLEIDHKAVTRGFVDSAPVLERAWANRAGLGWIGKNGMLITRKQ
                     GSFFFLAEILTSLEPEYDDPFAGNYCGNCSECLDSCPTGAIAAPGIIDANRCISYLTI
                     ENKGEIPESLRGKWDKWIFGCDACQDVCPWNRFSVGHAEPAFVPTFELASMQTGDWLN
                     LDKERFGSLFKHSAVKRCKYEGLKRNIDFVNAKEIHDSDCDS"
     gene            19240..20520
                     /locus_tag="PKI35_06900"
     CDS             19240..20520
                     /locus_tag="PKI35_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNX44148.1"
                     /translation="MSINHKPSVSAIDIRSEIGELRGVVLHTPGNEVENMTPNSARKA
                     LYSDILNLPVALKEYNQFRGFLSKVTKTYEVRELMLDVVNQDHHRKALISNIIKRESL
                     PGDLEGMLLSMKPARLVSALLEGVEMPKDNFTRFISKDRYILEPLHNFFFTRDASVAI
                     HNKVLISSMASPVRMRESIIMDAIFTNHPDFQAKTVHPEGSCHSCDDFSFEGGDILIA
                     REDVLLVGTGLRTTSRGIDFIIDYFKQRKSRQHIIVQELPYKPESFIHLDMVFTFLDR
                     NLCMIYEPLVLHPSRYLTIHIELDNGRVKSISEKPNIPAVLKELGMEVELLYCGGRHE
                     RVNQDREQWQSGANFFAVGPGKVVGYGRNVRTIEELSRNGFAVIRANDILHAKVDIAN
                     YRKYVVTIDGSELSRGGGGCRCMTMPVKRAPVNW"
     gene            complement(20534..21094)
                     /locus_tag="PKI35_06905"
     CDS             complement(20534..21094)
                     /locus_tag="PKI35_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNX44149.1"
                     /translation="MKIVIGVTGASGAVYAKVLFDQLAGLSGQVEACGIMFSDNAKQV
                     WQFELGNDDWLRLPYPVYDIHDFYAPFASGSAGWDAMIICPCSMGTLGRIASGVSNDL
                     MTRAADVMLKERRKLVLVPRETPFSLIHINNMKTVTEAGGIICPANPSFYSRPATFEE
                     LAAAVTGRALALAGFKLEGFRWSEPK"
     gene            complement(21134..21745)
                     /locus_tag="PKI35_06910"
     CDS             complement(21134..21745)
                     /locus_tag="PKI35_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX44150.1"
                     /translation="MSYKNLLIVITIFILTGMSVRAQRFTGGVAAGVVGSQVAGDTYS
                     GFDKAGIYAGVWVNLPAEEHSAWQMELDYIQKGSRRNPDPKSPDPTYFIMRLGYVELA
                     FLYQYRLASGLLLEGGPAFSFLLHHFEERDYQPTGDYPFRLFNTNATFGVGYKINDKM
                     TVHFRTDNSIYSIRTHPSNGNVGRFFDTGQYNDALILSLSYTL"
     gene            complement(21848..22447)
                     /locus_tag="PKI35_06915"
     CDS             complement(21848..22447)
                     /locus_tag="PKI35_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNX44151.1"
                     /translation="MSRCILVLLLCLSIQVSLPAQTDAGKPGFENICLSDAEQWLARL
                     INEYRKENHLKPVAISRSLSFVAHTHACDLAENYKHSDECNMHSWSDSGNWSACCYTH
                     DHKQARCMWDKPRELTGYPANGYEISFYTTRKYSSPQALAADILKGWKESKSHNEVIL
                     NKGIWKSVAWKAMGIGINENFATVWFGEIADPEGVPCQE"
     gene            complement(22520..23620)
                     /locus_tag="PKI35_06920"
     CDS             complement(22520..23620)
                     /locus_tag="PKI35_06920"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44152.1"
                     /translation="MNRLPIFLILFWLTGNLAVAQIRTETYDDGTKKAEGNLVNGVKQ
                     GLWYEFNEKGIKVAEVNYVDGIKEGKALTWFTNGKKQSEATYRKGKLNGMAYWWNEEG
                     KPDMTGRLTDGLHDSAWIFYHPNGAVGSHGNFVNDKQEGPWEYFYESGSIWKKGSYEN
                     SLKQGKWTFYYDDGKIHHTGSYVADKQDGLWMSWFSNGQVKDSGYYDNGNMTGCWRGW
                     YENGKPQYVAEYEKNALRDIYRSWYDNGQKKSEIHYLNGIKDGSSLEYYSNGQLKEKG
                     SYAGGERNGFFVYLDENGLKIKEESYVHGRPDGYWASYYANGRKQGEGTYLNLQKNGT
                     WQYWDENGKLVYKGVFNNGQKVKEKRYGSEKK"
     gene            complement(23681..24655)
                     /locus_tag="PKI35_06925"
     CDS             complement(23681..24655)
                     /locus_tag="PKI35_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNX44153.1"
                     /translation="MVTLDEIKIPIAHHIEEFEKHFRGSMKTPVPLLTTITNYILKRK
                     GKQMRPMFVFLSAQVCGEVNESTYHAASLIELLHTATLIHDDVVDDSNERRGYFSLNA
                     LWKNKIAVLIGDFLLSKGLLLALDNNEFQLLKIVSDATREMSEGELLQIEKARRLDIE
                     EPVYFEIIRKKTASLIASCCEAGAESVKADEETRARMRQFGEYTGIAFQIKDDLFDYE
                     KSLNTGKPSGIDIKEKKMTLPLIYMLNKVSWAEKRYAINIVRNHNTEPERVAELIRKV
                     KESGGIDYAAGRMIEYREKALDILCTFPDSPSRKALGQLVVFTTERQH"
     gene            24822..25601
                     /locus_tag="PKI35_06930"
     CDS             24822..25601
                     /locus_tag="PKI35_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX44154.1"
                     /translation="MSGILFDEIVFGPVRSRRFGVSLGINLLPLDSKLCSYDCIYCEC
                     GLTEHAAGHKPKLFSGAEIRNSLQQRFEALSQESLAPDNITFAGNGEPTLHPEFAAII
                     DDTLALRDQYFPEAKITVLSNATMLNRPAVVEALKKIDNNVLKLDAGTDETYRAINRP
                     LSPVTLEQVVDRLAAFEGDLTIQTLFVRGSVDGRMIDNTTEKEVSLWLGHLQRIRPAL
                     VMLYPIDRRTPYASLEKVSEAELLALASKVESIGFKSEVFY"
     gene            25703..26101
                     /locus_tag="PKI35_06935"
     CDS             25703..26101
                     /locus_tag="PKI35_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44155.1"
                     /translation="MLEYEPKILVAFAETLSGNTDIFNWLLNNGYPELAALSATLRGS
                     EEASGWLMKNGYQHFAALDQAIDGKMEAYLWLVRNDYRLLATLADACNNKPEAIRWLA
                     ANDLHIFIMIAEKFRGFRDSQTFDYHKIHF"
     gene            26174..26749
                     /locus_tag="PKI35_06940"
     CDS             26174..26749
                     /locus_tag="PKI35_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNX44156.1"
                     /translation="MESIKGTQTEKNLLKSFAGESQARNRYMFFAKKAREEGYEQIAA
                     FFEETARQEETHAKTFFKFLEGGMVEITASYPAGVIGTTAENLKAAAEGENEEWTTLY
                     REFGEIAEKEGFRKIAVAYKLIATVEKEHEARYLKLLKNIEEGKVFESEEETVWVCRK
                     CGYHHKGKKPPKNCPCCEHPEAYFEREAVNY"
BASE COUNT         7671 a         6171 c         6425 g         7017 t
ORIGIN      
        1 tcgcccccgt ctggccctgg catacaggcg acgggcccgt cggcgatacc gtcggggtgg
       61 ccggggtacg gattatacta acagagatag taacattatt ctgtgaaaga taataccacc
      121 atgaattgat ggtattttca tctcctaatc ctcctgaaga gctaaaggac caatcatgac
      181 aaccggttac aggcggttcg tcatcataca tacaacctgt aacggaatta tctattcctc
      241 ctagtccggg atttgtatca tcccaataaa ttctaagttt tgttccatca ggtggaaggg
      301 gatccggaag tggtttatga atatctactt taatgccgtc ttcattacct tcaacatcat
      361 ataatggcag attagtaagg ctgttaaggt aatcaatact catcgaaatt gatgctccgt
      421 tgttcacagg ttgtcctgaa ccattattac cattccaggt aatgagattt gagccggcta
      481 ccacattctg attgagataa acatcgtttg ggtcacctag gccagccggg ggaagatcta
      541 tcgataatgt tacttttcct ggtttatcga cgacaatttc aaaaataaca gaaccatcac
      601 aggaactgga tgtttgttgg gcgctaataa tctgacctaa aatcccggtt ggaaacagtg
      661 ctatatccgg ttcatttaag aacattttat attgcggtac aacacctgct ttgccttcaa
      721 tcgatttgcg ccgcgtttcc caatcaccgg tagtgctggt accccattcg ttgcaataga
      781 tatcgcaagc gccaacccgc atgccattgg cattaaattt ggtaacaata tggtcatcag
      841 agtatacata gaatattcct ctgaaattat cgaactgatt actatgcatt gcatagatct
      901 gccaaacttt tgaccatacc cttccgggaa taggtgtgta tgaaccaccg ccatttgaag
      961 ttgcaactgt tatatcgaag tactcaattg tagcagaacc actgaagttg ccttcaaaat
     1021 caatagaata atcaccttcc attgttggtg tgaacactat aggttcatag cctgaaggat
     1081 taatgacatt gggaccagaa actgcctgat tccaattgga aatataacct ggaccgccag
     1141 tcgggaaggt ttgagctgct gagtaagtcc cgtcaggata tcggatataa aatgaagaaa
     1201 tattatttac ggaattaagt ccaataaata cgacttcgtt tgcccagttg ctgctgacgc
     1261 taaattttaa tctcaggtta tcatcggtat ttgaatgcaa tgcccatgga aggtaagtac
     1321 tgccagatga taggtagaga caacctcttg ttgtgggaac tattgcaggg tcagagggta
     1381 ataactcttt tgtcccttct ccaaaacttc tcaggggaga agccagaaat gggatcagta
     1441 acaaggccat cagcgcagtt gatgcatgct tgctgacaga ggggcatata tatctgatga
     1501 agcgtaaaaa attcttcata ggtgacggta aggcttaata aggtaatgta attaagatat
     1561 ttaggacgta aagataggga caatagttca attctgcttt cggttcagca gggtttttag
     1621 ctgctgatgg tactatcaga ataacaatga attaaccgaa accgatcagc ggccatttgc
     1681 acccgggaag gcatagaaac agtaaatttc aatatatatc cagcctgccg gccgagtgta
     1741 tcatttcttt aaccatttca ttgaagtctg tttgttctgc caggctttca aggtcgatgt
     1801 gcatcctgta acgccagtaa tgactcggat ttcccggctg gttgatgcgt tccgaagggg
     1861 catgttgcag gcggagtttt tcatccatgc ccagaagatc ctgaatgggg aagatggccc
     1921 acatacttgg tgagtaaaga tgctggacaa tgatttgctt tacgatatcc gtttcacaga
     1981 aaaacggggc tttgccgtat ttgccgagga tttcgttata aaatttctgt gacatcgaag
     2041 gattttcctc ccaccatccc ctgatagtag aggtgtcgtg cgacgaaggt gttgccaccg
     2101 acaggtatgg ataatcggcc ggatggccaa aggtgatttt cgggtctttt ggcatccgct
     2161 gaacttccag gctcaggatc cccagttctt tcatcacatc gggaacgcat gccggcacca
     2221 tgcccaggtc ctcgccgcaa agcaacatat tggtcgtttt tttcagtaca ggcagttttt
     2281 ccatcgcttt cttccgccag aattcctcgt tgcgatggta gaaatactcc aggtaaatct
     2341 cgtcaatcag atgtttcgaa tgttcatcca actcctggta cgaccgggta taatggaggg
     2401 cattcctggg gaagaacgca tttccgccgg aatccggggc ttccaggaac acaacctccg
     2461 agataaggga atacaatccc tgagtaagtc tgacaaggcg gcttctttcc tcaggaccca
     2521 tatcaggctc gatggccagt ttttcttcga ctttccgctg tgtatccacc tcgggacgaa
     2581 ggtcataaca accgggataa aattctgtga gaaaattttc ctttgcataa gcagtgaggt
     2641 ctccaaagcg ttcgtacagg aaatgttcac ggatataggg acggcaaagc cgtttttcgt
     2701 cgaaccctaa tccccggtgc tgcaattcat caaggttgta tggaatgctc ggattgaaat
     2761 aacccatcag cccttctgcc tggcaggacg ggatttccca gatacggaaa aaccccagga
     2821 tatgatcgat gcggaaggcg tcgaaataat aagataattg ccttagccgc tgttgccacc
     2881 aggcataatt gtctttggcc atttcatccc agttgtaagt ggggaatcgc cagttctgac
     2941 ccttcgccga aaagtcatcg ggaggggcgc cggcctgccg gtccatattg aacaggtgcg
     3001 ggtgaagcca ggcatcaacg ctgttgcggt aaacgccgat cgggatgtcg cctttcaata
     3061 ctaccccttt cgacctggca tagtttgccg cgtcgagcaa ctggcggtgg gcatgatact
     3121 ggatgaaata atgaacggca atatcgtcgt aatgcggcga attttcatgg gtgagatgct
     3181 ccagcagttc ttcggcaggt ttgctgtact cgccccagcg gctgaagtcg ggtgtgttga
     3241 aaaggtcgcg caggtatgaa aaggcggcgt atggcttaag ccagtattcg ttatcggcaa
     3301 aaaatgcaag aaattcagga tcggtaagga attcggtctt tttttggtcg tagatcagct
     3361 tgaaaaatct tgatttgaga gccatcacgg cttcataatc tatccttttc agcgagttga
     3421 gatatttccc ctgtgcatca atgatctgct ggctgatatc ggcttccagc gtgccaatct
     3481 caagaaggtt gatgaaaatg ggatgcaggg cataaaccga tatggcggca taagggtatg
     3541 aatcctgcca ggtgtgtttg gcaacggtat cattgaccgg aagcacctgg atcagtttga
     3601 tgcccgtttg tacggcccag tcgaccagca atttaagatc ggtaaattca cccacgccaa
     3661 acccgtcagc gcgccgcaac gcaaacacag ggatagcaac tccggcccct ttccaggggt
     3721 attgagggaa gtcgaacttt tcatcgccga tttctataat atccggaatg tcgcctttcg
     3781 gcaaagcgac gacacggtcg gctgattttt cccaaaatac ggttttgccc tcttcgtcca
     3841 ggaaaaggta tttgtattta acgggaaact cggcgatctt aactattacc tcgccgcacc
     3901 attccggatg atcgggattt cccaaaggaa tggctttccg gtcgtcccag ttgccaagcg
     3961 cttttgcgtt tcctgacacg ccaaccctgt ggccgggctt tatacggaca acattgggct
     4021 taaagcgcag gatgacggaa tccttccgct cgccggtctc aatcttgggc gctttgtgag
     4081 ggtcccccgg tttcaggatc gcattcagga atgcggatga gtgtaatgca tattccggat
     4141 cggaagcagc ccgccacctg tcggacagta agatggtttt atcatgccgt ttttcaggat
     4201 ggaataccct gtcggggccc cattcctgca ggaaagtgtt ggaatttccg tcctttacga
     4261 agtaacggta gcgaaaatct ttatcctcat tcaactcaag gcagtatgtc cataaaccgt
     4321 ttttcgcgtt gtgcagggtc atgaacggtg ctttatcggg acatccgttg cccaattcag
     4381 gaacagaacc ggcaacaata agctgctgac cgggggatgt gttatactcg atgtggaacc
     4441 ggagtaacat aaaccaatca gtattaggat ttttagatgt agtgaaatta gtgaaaatgc
     4501 agataccggt aaataatttc tgattcctga aaaagaaggg ccatgcccgg aaagcaggat
     4561 cagcatcaac aatgttttaa aagggatttt tttgcctgcg ctatccctct cacaagcctg
     4621 ctcatgatcc ctccgtggta aatggaaata aaaaagctgc ctgttggcag cttgatgaaa
     4681 cttagtgacc ccggaggggt aacaatggct attttaaccc ataaacgagt taatgaagtt
     4741 attatgtaaa tatcacataa atagttaatt atataccatt tcattagtta ataacttttt
     4801 ctattatgtt attcttttgt ttgtatattt gtgtgtgaaa attatatgac ccctccgata
     4861 tgaaacactc aataacattt ttagtggagg caaggaaaca acagcaacca tttgcaccca
     4921 ttaatgtaga tgtgaattat acaggattgc ggatccgcta ctataccggc taccggctaa
     4981 gtaccgaaaa ttgggtatat aatggatcat ggataatgga taattgggac gtaaatgccc
     5041 accgcgttgt tagtggttac aaagcatacg acggcaaaac ccaggttatg gcgatccagg
     5101 ttaacaggaa acttgacaag gttaacggca atattgccga tgacctattt aatactgatt
     5161 taccaccttc aaaggatgcc gtgttaagct atctggatag catattcaaa ccctataaaa
     5221 aacaaaagga aagggctgaa aagcaaaaag agttatcatt ttggcagatg tatgaaagat
     5281 actgcaaaga ggcgaaggta tcaccggggc ggcgtaaaca gataaaagtt actatgaacc
     5341 acctgaaagc atttgatcca aatttgacct ttgcagcggt aacccctgaa aaacttcgca
     5401 agtttgaaag gtacctcctg agtgatgatc cgccgaaaag taaaaacacc gtttcagggc
     5461 aaataaagaa attccgggct ttctggaatt gggcaaagct tgaacgatct aaccttcatt
     5521 atccttttga aggctacaaa atagacgctg aagagtacgg ggagcctata tttctgacta
     5581 aagacgaaat agaaacctta tacagggcaa agatcacaga cgaacgtttg aaacggatcc
     5641 gggatatatt cctgtttcag tgttatgtcg gttgccgtgt tggtgacctt gtgaagctga
     5701 caaccgacaa tattattaat ggttcagtgc aatatattcc gcgaaaaacg aaagataaca
     5761 agccggtcac tgtaacaata ccgttaacga aacgcgcaaa agagattatt tcgcgttaca
     5821 aggtgcccgg cgggatgctg ttgccgttta tatcaccgca gaaatataat gatgccatac
     5881 gcgacctatt taagaataat aaagtgaaat tagaccggac tgttatccgg ttgaaccctt
     5941 tgaccaggga agaggaagcg gttaagcttt gcgatatagc cagctcccac ctttgccgcc
     6001 gtacttttat cgggaacctg ttcgggaaag tcgatacaag tattattcag agtatgaccg
     6061 gccacgcaaa agattctaaa gccttcgggc gctattattc tgtcactgat gaattgaaac
     6121 aacaagctat taaaaacctt gaatgatatg gaaaaacagg aatttctcaa aaagcttagt
     6181 gaagtaattg acgaattacc acaactcgtg gatgaggtaa gccggaaaac aaaagaaatt
     6241 gtaaaatatg aatttcagca gataaataaa aaaattgctt tttcgccaag tgataaggat
     6301 gatattctga aagagtataa aaagagggtg attggtgaat taaatgtaaa tatgattaat
     6361 gatgcggggg aactaaatgt taaatggtac aaggattttt tgatacagaa acttaacttt
     6421 tgttctaagt gggaatggga ttcatcaacc cccgaaaatc gtttaccggc accgttacat
     6481 atgatactat taccgaggga acacgtggtt atagagataa cgaaggttat ttacaataaa
     6541 gaattaatag actattgtaa ttaccatttg caacacaatg aagctgggct gggtttggct
     6601 gcaacaaagg ctaagactga atgcgatgtt attgctaagc aattagaggg taaaggattt
     6661 atcccgcgtg gtgatggtgc aaaattcatt tcaattttct ttcctgatgg aaggccgttt
     6721 gaaaaggtta catggctgaa atcccggaaa gactgttttt ccttaatgat ggaaatgaat
     6781 gatcctgata ttaaaccaaa agtaattaat aagtatatta atcaggccgg tattgatcct
     6841 aagactaaaa aagttaacac aaaaccatta aggtcacagg ataaacaaaa ttgtactgca
     6901 tttcttagaa ggttaaaata actgatttgt tatgattaat attagcctga aaccgctggt
     6961 tatcaggctt ttttatttat gtgtgtttta attttgttac ggtaaacata acaaacaaat
     7021 aaatcaaagt caaatctaat tggtattatc atattatctt taccagtgtt tattaaagaa
     7081 taagaattat gccaatggta gtattaacat ccgatgaact cacccacatc atcagagggg
     7141 ttatttccga cgaaataggg aaaaggctgg acgccaaacc tccgcgacca acaataaaag
     7201 gcattcacgg actggccgaa gctttaaatt gttcgatcgc caaagctcaa aaattgaaaa
     7261 atagcgggca aataccatat ttccaaaacg aaaagctctt gctattcgat tttgaccagg
     7321 tgatggatgc cctgaaaggt acagacatac accgccgggg aaggcgtcct cgtcacgttg
     7381 agccggagcg ggccgttgag ataaggaggg taaatgagag gagttgataa aaacaccgaa
     7441 gcccccaccg ggcaggatgg aggcttcaac aatgctttgg taggcaaacg acagcgcaaa
     7501 gatactgcat ttcaggcact ttccgccctt tatttaaaag aaaatatccg caaatacccg
     7561 tccctcccat accgctgtgt cccaaaatgg aacgactgta ccagcaacgg actgacccga
     7621 atgataatag actttttaag attatccggg caccaagccg aaagaatcag caataccggg
     7681 cgaatgctgg acgggcgaaa gatcgttgtt gactgcatag gcaaccggcg tttgattgga
     7741 tcgaccgcct ggatacccgg aaccggaagc cgcggaaccg ctgacatatc cgcgacaata
     7801 aacggcagat cagctaaaat tgaggtcaaa tgccaggcca ccaaagataa acagcgaccg
     7861 gcccaggcag aatatcaaaa gcagattgaa gccgccggag gtacttacta tgttgctcag
     7921 acttttgcgg gctttttcaa ttggtataat aaaacattcg gaggagccaa ccaatgaacg
     7981 agaaattaat tcctccccct gacctgaatt taaacggcga accgttaccg gaaacggcaa
     8041 cccataccct gaaaaccttt gaaccttacc ggattactga tacaacccaa ataccagcgc
     8101 cggtacctgt tataaaaata agcggtgaaa caatcagcac accggaacac attacagtca
     8161 tttccggggc acccaaaagc ggcaagacat cattttgtag tattttgata gcgggtgcaa
     8221 tttcaaagac tggtgaaata tttgataccc ttgacctggt taacgtctta ccaaaccgaa
     8281 accaattagc ggtaatacac tttgaaaccg agcaggcacc ctggaaacac caggcgaacc
     8341 aagccgggat tttaaaacgc gccgggctgg atcattgccc tggttactat ctttcctata
     8401 atatccggca aataccgatt gaggaactga atgagtttat ttccggggta tgccaggcag
     8461 cagccgcgca attcgggggg atattcgttg tctttgtcga tggtatagca gatttaatcc
     8521 gcgacccaaa cgaccaggcc gaatccttcg atgttgtcaa atttttggag ggtatcgcac
     8581 aggaatattt ctgccctgtc attgttgttt tgcacacgaa ccccggcagc gacaaagagc
     8641 gcgggcactt aggcagccag gtacagcgca aagccgaaag tgttttgcag gttaaaaagg
     8701 agggtaatgt ttcatattta gaccccaagt tattacggca tgcaggagac gacattccga
     8761 aggtacaatt tcagtatgac cgtgataagg ggtaccatgt tcaatgcaat gttgacaacc
     8821 cgaccgaccg acgggcgatt cagaaagttg agaagctgga ggccttagca ggcaaggtat
     8881 tttcaggaca ggcagccttt aaatatagtg atgcgatcaa tgtaattatt cgacataccg
     8941 gatacggtca aacgactgca aaaaggctgt ttacagaaat gaaggcatgc gaactaattg
     9001 agcaaggtaa tgacaacaat tggcgaaaag ttaacaaacc ggtatagtgg tatagaaccg
     9061 gtacattgac cctaattata ccggtatagt ataggtcaat tactataccg gatattgtac
     9121 cagtgaccta aattaaaaag tgacctaaat atgttcagct acttcaccaa ccacagtcaa
     9181 aacatccccg cgggggtaat aacattaccg gaactacacc ggcaattaat tgcaccggat
     9241 cgggagccgt ttctgagggc tgcaaacctg gttaaacatt tgagggctgc aacggatgaa
     9301 gccgaggcga aagcgattaa aagccaattg cctgcaattt gccccggtgc aacacttgac
     9361 accagggcga ccggtgcaac acccgaacaa caaaatatca gttacagcgg ctttgctcaa
     9421 attgatattg atttgcacga caacccaaat atgaccgatg cagcggaggt acgggataaa
     9481 ttggcccaaa taccctacat agcattaaca gcaatatcag ccagggggcg gggcgtttgg
     9541 gcattgatag ccctgaaaga accgggcaag ttcacggaat atgccgaacc gattaccgag
     9601 tttttcaaac gagccagggt aacgatcgac aaaagcaaaa gcaagcggcc aacggaatta
     9661 cgatacttcg cccccgatcc cggcgcgatc ctgaaaaatg aatatcagtt gtttccttta
     9721 atccggccac agtccaaacc aaaacccgcc gcaggtaaca ggcagcacac cggggcaacc
     9781 tttgaacagg tgcaacgctg ggtatcagaa acaacaggct ttaacctggt tgacggtcaa
     9841 aaacattatt acctgttttg gttagcgtat ggcctccgga aaaacggaat ggatccacaa
     9901 tctgtttacc aactgattta cgataatgtg ctgccggaaa gttgcgtgca ttcaaactgc
     9961 attagcggcg gtatagcgta tgcaaatacc aagggaatat acacccccca aaccgcgaag
    10021 acagcccccc agatcacgca cccaagggta acaaaaacaa ccccggccag cacacgcgaa
    10081 ccggttacgc cacgtcaaac caaactgata acaaactgca tcaagacggt caccggcgat
    10141 atttaccggc cattgccata tggcaggtac cagcgttaca ccggtgacac tcccggcgaa
    10201 ataatcggat ttggtgacag cctaaggctt ataaacagcc cttcgtttga ttgcctatac
    10261 actcttaaac tcaattagtg atgaggtact tcacgaaact cacagacctg gcaaagcatt
    10321 acaacgtgga tgaaagcctg ttgttcaagg cctattgcaa gatgactggc aacagcgacc
    10381 ccgaaaacat gagccggtta tggcagcaaa caaacggcga ggtactactt gctccgtgtg
    10441 aagttgaaaa aataatcaat ataatgatgt ataactaatc attaagccaa tgcaaccatg
    10501 tacacgaatg aaaaaagatc gtgcagcaaa tcgcacgaaa tgcagccaaa tggcaaggtt
    10561 taccaccctg gcattaatgg caacaacgac gctgataatc tttgccctga taagttagta
    10621 catgaataca accttaaata attgtaaatc aatgagatac ggggcgggta aattcctcta
    10681 gactttcggc ttcttaatcg tcgatggaaa ctaacacata tacatgcaat aaaaaaaagg
    10741 atgttatata ataaccaaaa tgctatgaaa aaaacactaa aaactgaaaa aattcaacgg
    10801 ggaacctatg cctcgagccg tgacaagtcc gcaaaaggcc tcccggttct ccttctctct
    10861 gaaatcccgg aagccccggc acacctgaca gcctccgccg ctgagatatt ccgtgccact
    10921 tgccagtctc tcaaaaacgc gcggaagttg accgccgaca cccttaatct gattgctgat
    10981 tactcttatt tcagcgatct ggcggctgta tggcagcagc gttctgaaaa gattgatcag
    11041 ttgatgggtg aatcttttga ccccgtaatg atgcgaaaat tcgtagctgc aaaccgtctg
    11101 agcaggcaga acagctccca tgcctgcaaa cttgcttcgc agttaggcat attaccggga
    11161 gatcgtaacc cggaaaggga tcaggacagc agctttgaac gagaattttt acaatgatgt
    11221 gcgttcatca gcgccggcca gccgcttcct gttcgcggca agtagcaaaa cagggcggtc
    11281 tggcctttct taaaaattta ttcaccaaac ataatttacc atgaaaacat taaatgaagc
    11341 cagggagcaa cttgaaaagg aactttcaaa cctggagagg caacagcctg gagccgattc
    11401 aatgcaacta atcggagctg tccggcagct acttggcctt gcaaacatgg ggcttctgcc
    11461 tgacgtgagg gcaagattaa cggagctcaa aatgtcatcc ggacccgatg gccggacaaa
    11521 taggattgaa aacaactata caaaacgtta aagccatgaa ttacaaagag caaagaaaca
    11581 cggaccggga actacgatat attgacggga gccgccacgt tgttggcctg gctgttgtct
    11641 ttaataagcg agccccgatc ggcgactttt ttgagcaggt agccccggaa gcaattgacg
    11701 atgacgtact gaaccaggac gtgagatgct tgttcaatca cgattctaat ttcgtcctgg
    11761 gcaggacaaa ggcaggtact ttgaaactga caaaaacagc cgaaggcctg gagtactcac
    11821 tcgatatgcc ggtaacgacc ttcggcaacg aggttctgac agctattaaa ctggggaacg
    11881 ttagcggttc atctttcggc tttaccgtga agaaagacga atggaaagaa cttcccggcg
    11941 gtatttattt gcgcacgatc ctaaagatcg ggaaattgtt tgatgtttcc ccggtcactt
    12001 ttccggcgta tgctgatacg actgttggga tccgcagcct ggaggcctgg aaacggtcac
    12061 atggcggtaa atcaatccag gattacgaac aggaactaaa aaaaatggaa ttagaaatga
    12121 accggccgca gccgcaccgt accaccttgg cggaggctga acggcagttg aaagcattaa
    12181 gaccataatt attaacttaa aaacaaaaat acaatgtcaa agagtgtaaa tcaattgatt
    12241 gcggaggatc cgtttgaact tcaccgcacg ttcgtttcaa tccaggccag tagccgggag
    12301 actgataacg aggctattta tcagaggact ttaaacgtgt tacgatcccg cggaggcgtc
    12361 gatttttatg ccgattcata cgccgatttc gcggcaaatc caatcacaag atgccccgac
    12421 gatgtccgcc atgccgattt taaagccggg cgggtaatta ccgatcgtat tgaagcgttc
    12481 caccacaatg ccgctgtttt aattcagaaa gtaaaacacc gcgttaataa cctccgccaa
    12541 accgaagagg acgcctttac ccttgagaac cttgataacc gaaccttcaa agactttgcg
    12601 caatatcggg attattgcag caaagtgcag gccgggcaaa ttgagcggcc accgagggag
    12661 aaaaagccag ctcccgatcc ggaagctgta aaacgtcgga aattctacca ggaggtaatc
    12721 caaaggcaaa taaatgcagg tcagtcactt gaaactgcat accatgctca gaatattcag
    12781 gttccgggaa tattcggttc atttaaagag tatgaaacgt accacaaaca tccggaggtt
    12841 ttatgaaagc aacagagaaa gaaaaacaag agttttacag cttagtccgt actgtttggc
    12901 gtaacccgcg tttcagtggt cagccggcaa tgtttccgtt ccatgaagcc gtgaagcttt
    12961 ggagaacaaa tcacacctgt ttaccatttg aaacggtaga agaatttcgg gcatacggag
    13021 ggaattacgg ccagcgttcg gcaaccccta ttcagcagga acaacggcaa tatctgaacc
    13081 aacgtgataa aatccgcgta ttgaaaaaca cccattaccg gcagtagtga cgccgaaacc
    13141 gtaagagggg aaagccccgg aagattggag gtttcccaaa aacataccta taaaataatt
    13201 tcgaccacac tgggaagtta aagaatcaaa gttgagccgg gtaaatcccg gctttttttg
    13261 tatcacccac acaataataa aaccaaaata agtgtgttaa atagtgtgta aatgacatta
    13321 aaaaagcctg caatctattg atttacaggc ttataaatta atttctaagt gaccccggag
    13381 ggattcaaac ccacgacttt cagaaccgga atctgacgtt ctattcagct gaactacggg
    13441 gccggtattt caaaatgcaa aagtataaaa tccaagcgca tttccacctg atgatttaat
    13501 aattcctgat gcttgtcctg tcagggtggc cgatgtggtc agttaaatgg cccgaccttc
    13561 aagttatatc gtatctttgt aagtaggtag tattaatcat aaataattca ataacatgac
    13621 agacaaggtt tttgataaga ttgcccccgg tgttgttacc ggcgacgatg ttcaggaagt
    13681 tttccggatc gcccgcagga atcattttgc cattcctgcc gtaaatgtgg tgggcaccga
    13741 ctctatcaat tcggttcttg aaagcgcccg ggaagtcaat tcacccgtta ttattcagtt
    13801 ttcgaatggc ggcgcacatt tcgtagccgg taaattcctc aataacgacg gggagcaggc
    13861 tgccattacc ggagccatat ccggtgcaaa acacatccac atggttgcca aaatgtatgg
    13921 agttcccgta atcattcata ccgatcatgc tgccaagaaa cttttgccct ggattgacgg
    13981 acttctggat gccggcgaaa aacattttgc cgaatgcggc gtaccccttt tcagttcgca
    14041 catgcttgat ctttcagaag agcctctgaa ggagaatatt gaaatctgta agaaatatct
    14101 gacacgcatg agcaaaatag ggatgacgct tgagattgaa cttggcgtta ccggtggcga
    14161 agaagacggc gttgacaata ccggcgtcga tagcgcccgc ctttacactc agcctgaaga
    14221 tgtggcgtat gcttatgaag agctcctgaa aatcagcaac aggtttacga ttgccgcctc
    14281 attcggaaat gttcacggcg tgtataaacc ggggaatgta aaactgcagc cggtcatcct
    14341 gcataattca caggtataca ttaagaataa attcaacacg gaagaaaacc cggtgaattt
    14401 cgttttccac ggaggatcgg gatcaagccg cgaagaaatc cgcgaagcca ttagttacgg
    14461 agtggtaaag atgaacatcg ataccgatac acagtgggcc acctggaaag gcgttctgga
    14521 tttctacaag aagaatgaag gttacctcca gggtcagatc ggcaacccgg aaggcgaaga
    14581 taaacccaat aaaaaatact acgatccgcg caaatggctg cacgaaagtc agaaatcgat
    14641 ggtattgaga ataaaggaag cgtttgaaga cctcaattgc ttaaacagga attaaacgct
    14701 gaatgaagcc aataaaaaag ccccgtcatc aggggctttt tcattaatgg gatgccatga
    14761 tcagcgtttc tttcggattg atgctcaata ccgggaaagg agatgtatta accatttgct
    14821 gggcataagg gcctatcagc aggttataca tcttggtttc ctgttcggtc atgatgctga
    14881 tgagatttgc tttaaccttt tcggcataat caatggtagc taccgtgagg ttatcggcct
    14941 gcaccgaatc gagcacatac gaaatttctt cttcatcaag atactgaatt acctgttcgg
    15001 catattcgtc aatggtatgc tgaacggctt ttactttatt tgaataaagc ttaaggatgt
    15061 ggatttcggc ttcaaagagt tttgccaggt aagcagtgaa aggtaccttt tgccgtgtat
    15121 cgagcgtgga atcaagcggc attacgatgc gttcgagatc gtgtttgatg ctgatgcccg
    15181 accgtagcgt gattaccgga cagtgcgaca ccgagataag ccggttggca ttgctcccga
    15241 tccagaattg ttcaaatccg gaagcgccat gcgtgcccat cacgatgcag atactgttca
    15301 tctccttggc ttcatcaatg atttcacggt atactttccc ttcgcgtatc acgtaatcaa
    15361 tttcactttc gggaagacgc ggtttgtact tttcgattaa tttggcaaat tgccctttga
    15421 tctggtcgat gagatctgaa gaggtatccg agtaaatcgt ggtctttgtg acactcggat
    15481 tgttaaccca aaccatatga aggtcgagta aacccctgtt ggcaattgaa acggcatgtt
    15541 caagggcgtt gattgagcaa tcagaaaaat caacagcagc gattaggtta ctcatatgct
    15601 ttttttataa agtgtttttt aaatatttat tgataaaatt aatgaatatt cacttaaaaa
    15661 gcaaatctca ttcataaaag gttaataaag aattttaccg gccacttaag tagcaaatta
    15721 tcaatagttt atcattgatt caagaaaaat tttcagtcct gctcactgaa aagaatccgg
    15781 tctttaacag taaaagcatt gggatagtca gcctgtattc ttttctgaaa acctatagcc
    15841 tcactccgcg tgcgaaaatt acctatccgt atccggtaat aaggctgttt gaacgaaaga
    15901 taagctttta cttctggata tttttcctgg aaggtttcaa gcgcctcagt cgccctgttt
    15961 ttgctgttat taccggaatc aaaaaaaatc tgtaccctga agccatcaaa agaaggatca
    16021 cgctggttta cttccttata aaggtcggca agttttacca ttttatcgtc ctgataaatg
    16081 gtgaccttac cgggtgcgtt ttgttgttcc tgtgccctgc tgaattgcgc tcctgccagc
    16141 aggatggcaa acaggatcag aactgatttt ttcattggta tcagagtaaa aatctttcta
    16201 ccgggtggtt gcttaaaaca ggaatttcgg aatagttgat gagttgttgt gaaaatttgt
    16261 caagcaatac cccgctggct tcaacatcgc catctgtcat aattgaaatc aggtcggcat
    16321 caataaggcg ggaatgttca ataatgctgg tggcaatatt atcggtgttt tgtttctcgg
    16381 taataaaagg tattttgtga tcgtggaagt acttttcaac tttcacaacg gcgccgtcaa
    16441 ttacacgctg aacggtcttg agcttactgg agttcagggc gatgatattc acttcggcgc
    16501 caaaggaatg agcaaaagaa gccgtaaagg gcactttatg gagcgtaagg gccgtatggt
    16561 ctacgggcat tactatcttt ttgatggggc ggtttatatc atagctggtc cgaacggtaa
    16621 ttgtgggaca aggaacgctg gtaaccagcc gcagggcgtt actgccaatc cagaattcct
    16681 caaaacctgt tatgccgtgc gttccggcta tcaccaggtc agcgcctgaa agacgcgcct
    16741 gacctgcgag ttcctggtac acttttcctt tcctgagttt ataacttaac ttaccgccaa
    16801 gaagtttata cttttccgta tggaggatct cttccatatt ggtccttgct tcatcacgca
    16861 tttcggaact ttcggcctgc attagcattg cggggctgtc aacccagacc attgtgatgt
    16921 tactgttaca cgtattggct aaagcaacgg catattcaag ggcatgtaaa gagccctttg
    16981 aaaaatctat ggcaaccagt atctctttca tcgcttcagg ggattggtta gtccgggtaa
    17041 aagtataaat atttttgatg tgccgacatt ttcaaggctt atttcagagt tccacttaac
    17101 aaagttaaca ttcatgggaa ttcttacctt tgtacagatt catcttttgg aactatgtcg
    17161 ttgaataaaa acctggatgc taccggagaa tatatgacac cagccgaacg cgagattgaa
    17221 agggcgctca ggcctcctga tttcggcagt ttttcagggc aggcgggagt agtcggcaat
    17281 ctcaggattt ttgtcgctgc cgcaaagcaa cgtaatgaac cgctggatca tgtattgctg
    17341 catggcccgc ccggattggg gaaaactaca ctttcgtaca tcatcgccaa tgagctggga
    17401 gtcggcatta aaatgacttc cgggcctgta cttgataagc ccggcgacct tgccgggctg
    17461 cttaccggcc tggaaacgaa tgatgtgttg tttatcgatg aaatccatcg cctgagcccc
    17521 gttgtggagg aatatcttta ctcggccatg gaagatttcc ggatcgatat tatgatcgaa
    17581 tcagggccca atgcccggag tgtgcagatc aaactaaacc cttttaccct tatcggcgcc
    17641 acaacgcgtt caggattgct tacggcgccg ctccgttcaa ggttcggcat caattccagg
    17701 ctgcagtatt atgatgccga aaccctgaaa catattataa aacgttcggc cgggatactg
    17761 ggcgtaccca tccgtgacga tgcggctcac gagctggcac gtcgcagccg cggaacgccc
    17821 cgcattgcaa acctcctgct tcgcagggtt cgcgatttcg cacagatcaa aggcaatggt
    17881 accgttgacc tggcaatttc gcaatatgcc ctcgaagccc tgcttgtgga taaaaacggg
    17941 ctcgatgaaa tggacaaccg cattctggct tccatcatcg aaaaatttaa aggcggtccg
    18001 gtcgggttaa caaccattgc caccgccgtt ggcgaagacc cgggcaccat tgaagaagta
    18061 tatgaacctt tcctgatcca ggaaggattc ctgatgcgta cgccccgcgg acgcgaagca
    18121 accgaaaaag cataccttca tacgggcaaa atcaggaccg ggatcaacgg ttcgcttttt
    18181 taaaaagggc ctccctttcc ggataataaa actcaaccag ttacatgatt ccgcatgagc
    18241 tttctgtccg tattaaagaa aaagctgccg cattgggctt ttccggatgc ggtattgcaa
    18301 gagcagaaca tcttgccggg gatgaagaaa gattgaaacg atggctcgat gccgggcgcc
    18361 aggccgggat gcaatacatg gaacggaact tcgaaaagag aaccgacccg cggttactgg
    18421 ccgaaggaac aaagacggtt atttcgctgc tgtataatta ctttcccggg aaagagacga
    18481 attgtgaaaa cggtttcagg ctggcgaaat atgcgtatgg cgttgattat catgaactga
    18541 tcagggaaaa gctcgatatt ttgtgtaagg tgatgctcga aattgatcac aaagcggtaa
    18601 cccgcggatt cgtggattcg gcgccggtgc tggaacgcgc ctgggctaac cgcgcaggac
    18661 ttgggtggat cgggaaaaac ggcatgctga tcacccgcaa acagggttca ttttttttcc
    18721 tggccgaaat actgaccagc ctcgaaccgg aatatgacga tccgtttgcc ggtaattact
    18781 gcggcaattg ttcggaatgc ctggattcct gcccgacggg agctattgca gcgccgggga
    18841 ttattgatgc taaccggtgt atttcgtacc tcaccatcga aaataaaggc gaaatacctg
    18901 aatcacttcg cggcaaatgg gacaagtgga tttttggctg tgatgcctgc caggatgttt
    18961 gcccatggaa ccgtttttcg gttggccatg ctgaaccggc atttgtgcca acctttgaac
    19021 tggcatcaat gcaaaccggc gactggttga accttgataa agaaaggttc ggcagcctgt
    19081 ttaagcattc agccgtaaag cgctgtaagt atgaaggatt gaaaagaaat attgattttg
    19141 tgaatgcaaa ggaaattcat gacagtgatt gtgacagcta aatagccgga ttctgtcccg
    19201 gacttgttac catatccata cacccattag aaatgcatta tgtcgattaa ccataaaccc
    19261 tccgtatctg caattgatat ccgatccgaa atcggggaac tcaggggcgt tgtgcttcac
    19321 actcccggta acgaagtgga aaatatgacc ccgaatagcg ccagaaaagc gctttacagc
    19381 gacatcctca acctgccggt tgctctcaag gagtacaacc agttcagggg attcctgtcg
    19441 aaagtgacaa aaacctatga agtcagggaa ctgatgctgg atgttgtcaa tcaggatcat
    19501 catcgcaagg cgctgatcag caatattata aagcgtgaat ccttacccgg cgatctggaa
    19561 gggatgctgt tatccatgaa acctgccaga ctggtctcgg ctttactcga aggtgttgaa
    19621 atgcccaagg ataatttcac acggttcatc agcaaggatc gttacatcct ggagcccctt
    19681 cacaatttct tttttacgcg cgatgcttcg gtggccatcc ataacaaggt gctgatcagc
    19741 agcatggcaa gcccggtccg tatgcgggaa tccattatca tggatgcaat ttttacgaac
    19801 catcccgatt ttcaggccaa gacggtacac cccgaaggaa gttgtcattc ctgtgacgat
    19861 ttcagcttcg aaggcgggga tattttgatt gcccgcgagg atgttctgct tgtcggcacc
    19921 ggcctgagga ccaccagccg gggcatcgac tttatcatcg attacttcaa acagcgcaaa
    19981 agccggcagc atatcatcgt acaggagtta ccgtataagc cggagtcatt catacacctt
    20041 gatatggttt ttaccttcct cgaccggaac ctgtgtatga tctatgaacc gctggtgctg
    20101 caccccagcc ggtatctcac cattcatatt gagctggaca acggcagggt gaagtctatt
    20161 tccgaaaagc ccaacatacc tgcggtgctg aaggaactcg gcatggaagt ggagctgctg
    20221 tactgcgggg ggcggcacga acgggtgaac caggaccgcg aacaatggca gagcggggcc
    20281 aacttttttg ccgtcggtcc cggaaaagta gtgggctacg gccgcaacgt ccgcaccata
    20341 gaggaactaa gccggaacgg atttgccgtc atcagggcaa atgatatact ccatgcaaaa
    20401 gtggatatcg caaattaccg gaaatatgtg gtgaccatcg acggatcgga attgtcgcgt
    20461 ggcggcggag gctgccgttg catgaccatg cctgtgaaaa gggcgccggt caactggtaa
    20521 agcatgggaa atcctatttc ggctcactcc atctgaagcc ttccagttta aatccggcaa
    20581 gcgcgagcgc acggccggtg accgcggctg ccagttcctc gaaagtcgcc ggacggctat
    20641 agaaggaagg attggcaggg cagataattc cgccggcttc cgtcacggtt ttcatgttgt
    20701 tgatatggat caggctgaaa ggtgtctcgc gggggacgag aaccagcttc cgcctttctt
    20761 tgagcataac atcggcggcg cgtgtcatca ggtcgttcga aacgccggaa gcaatccgtc
    20821 ctaaggttcc catgctgcaa gggcagatga tcatggcatc ccaacctgct gacccggaag
    20881 caaaaggagc gtaaaaatca tgaatatcgt agacagggta gggcaggcgg agccaatcgt
    20941 cattgcccag ttcgaattgc cacacctgtt ttgcattgtc cgagaacata ataccgcatg
    21001 cttcaacctg accggacagg ccggccaact gatcaaacaa cacctttgca taaacagccc
    21061 cgcttgcccc tgtaacgccg attacaattt tcatgaatgt ctgtaatggt ttttagaaaa
    21121 tgatatttgc tgattaaagg gtgtagctca ggcttaaaat aagggcatcg ttatactgcc
    21181 cggtatcaaa aaagcgcccg acattgccgt tcgatggatg ggtgcggatg gaataaatgg
    21241 agttatcggt gcggaaatga accgtcatct tatcattgat tttatatcct accccaaagg
    21301 tggcgttggt attgaacaat ctgaagggat agtcgccggt gggctggtag tcgcgttctt
    21361 cgaaatggtg aaggaggaat gaaaacgccg ggccgccttc cagtaaaagt ccgctggcga
    21421 ggcgatactg gtacaggaaa gcaagttcaa catagccaag ccgcataata aaataggtgg
    21481 gatcggggct tttcgggtcc ggattgcggc gactgccttt ctggatataa tccagttcca
    21541 tttgccaggc cgaatgctcc tcggcaggca gatttaccca tactccggca tatatgcctg
    21601 ctttatcgaa accgctgtaa gtgtcgccgg ctacctgact gccgacaaca ccggcagcaa
    21661 cgccgccggt aaaccgctgc gcccggactg acattcctgt aagaatgaaa atggtaatta
    21721 cgattaacag atttttatag ctcatggacg ggtactatca tttgatttgc cgtaaaaata
    21781 gcaaaattac tgaaggagcg atatttcatg gtttatgaaa cagcaaattc aactgtataa
    21841 cccggtatta ttcctggcag gggacccctt ccggatcggc aatctcgcca aaccaaaccg
    21901 tggcgaagtt ttcgttaatg ccaattccca tggctttcca tgcaacgctt ttccatatcc
    21961 ctttgttcag gatcacctcg ttatggcttt tgctttcttt ccatcctttc aggatgtcgg
    22021 cagccagcgc ttgcggggaa ctgtatttcc gggttgtata aaatgatatt tcgtacccgt
    22081 ttgccggata accggtcagt tcgcgcggtt tatcccacat gcaccttgct tgtttatggt
    22141 cgtgggtata acaacaggct gaccagttgc cgctatccga ccaactgtgc atgttgcact
    22201 catcgctatg tttgtaattt tcggccaggt cgcaggcatg ggtatgggcg acaaaactta
    22261 acgacctgga aattgctacg ggctttaaat ggttttcctt acggtattca ttgatcaacc
    22321 gggccagcca ttgttcagca tccgaaaggc aaatattctc aaatccgggt ttgccggcat
    22381 ccgtttgggc cgggagtgaa acctgtatag ataaacacag aaggaggaca agaatgcagc
    22441 gtgacatgct tcgcggtttg tagcataact tgctgttgcc cgaaaaattg tatgaaggga
    22501 ggcggattgc ggaagccggt tatttctttt cgctgccgta tcgtttttct ttgacttttt
    22561 gcccgttgtt gaaaactcct ttataaacga gttttccgtt ctcatcccag tattgccagg
    22621 tgccgttttt ttgcaggtta aggtatgtgc cttcaccttg tttacgcccg ttggcataat
    22681 aacttgccca gtaaccatcg ggacgtccgt gaacataact ttcttccttg atcttcagcc
    22741 cgttttcatc cagatacacg aagaaaccgt tccgttcgcc cccggcataa ctgccttttt
    22801 ccttcaactg cccgttactg taatattcca gactggaacc gtcttttatt ccgttcaggt
    22861 aatgaatctc cgattttttt tgaccattgt cataccagga ccggtaaatg tcgcggaggg
    22921 cgtttttttc atattcagct acatattgcg gtttgccgtt ttcataccat ccgcgccaac
    22981 atccggtcat atttccatta tcataatagc ctgaatcctt gacctgcccg ttcgaaaacc
    23041 agctcatcca caaaccgtcc tgcttgtcgg caacatatga cccggtatga tgaattttgc
    23101 cgtcatcgta atagaaagtc catttaccct gtttcaggga attttcatag ctgccttttt
    23161 tccagatgct tcccgattcg taaaagtatt cccagggccc ttcctgcttg tcgttcacga
    23221 aattgccatg gcttccgaca gccccgttgg gatgataaaa aatccaagca ctgtcgtgaa
    23281 gcccatcagt gagtctacct gtcatatcag gtttcccttc ctcgttccac caatatgcca
    23341 taccgttgag ctttcctttt ctgtaggttg cttccgactg ctttttgcca ttggtaaacc
    23401 aggtcagggc tttcccttcc ttgataccgt caacataatt cacttcggct actttgatgc
    23461 ctttttcgtt gaattcgtac cacaatcctt gttttacacc gttcaccagg ttgccttcgg
    23521 ctttttttgt gccgtcgtca taggtttcgg tcctgatttg tgcaaccgcc agattgccgg
    23581 tcagccaaaa taaaataagg aaaataggga gcctgttcat ttttcaggag atttgagaat
    23641 tgctggagtg aagaattgta tcagtcggtt gaatggattt tcagtgttga cgttcggtag
    23701 taaatacgac cagttgcccg agggctttgc gtgaggggct gtcggggaaa gtacagagaa
    23761 tgtccaatgc tttttcccgg tattcgatca tcctgccggc agcataatcg ataccgccgc
    23821 tttctttaac ttttctgatc agttccgcca cccgttcggg ctccgtatta tgattcctca
    23881 cgatattaat ggcataccgt ttctcagccc acgacacctt gttaagcatg taaataagcg
    23941 gtaaagtcat ctttttttcc ttgatgtcga taccactggg tttgccggta ttcagactct
    24001 tttcgtaatc gaagaggtcg tcttttatct ggaaggctat gcctgtatat tcgccaaact
    24061 ggcgcatcct ggccctggtt tcttcatcgg ctttcaccga ttcggcgccg gcttcacagc
    24121 atgaagcgat gagtgaagcc gttttttttc tgatgatctc gaaatagaca ggttcttcga
    24181 tgtcgaggcg gcgggctttt tcaatctgga ggagttcgcc ttcgctcatt tcgcgcgtgg
    24241 catccgatac aatcttcagc agttggaact cattattgtc cagcgccagc aacagccctt
    24301 tcgacagcag aaaatcgccg atgaggaccg caattttgtt tttccagagg gcgttaaggg
    24361 aaaaatatcc gcgccgttcg tttgagtcgt ccacaacatc gtcgtggata agggtagctg
    24421 tatgcaaaag ttcgatgagg gaggctgcat gataggtgct ttcgttcact tcgccgcaaa
    24481 cctgcgccga caaaaacacg aacatcggcc tcatctgttt gcctttgcgc ttcaggatgt
    24541 agttggtgat ggtagtgagg aggggcacag gagttttcat cgaaccacgg aagtgcttct
    24601 caaactcttc gatatgatgg gcaattggaa tctttatctc gtcaagggtg accattccat
    24661 aatatatatt gatcggtaaa agtaacgatt atttatatcg ttgcgatata ccggctcgat
    24721 ggcgatcggg gaaggaggaa atacatcctg ctctccgatc cgggaagctt ttccggtttc
    24781 cgatgcctga ataaatgtac ttttgctgaa aaattgaaag catgtcgggt attcttttcg
    24841 atgaaattgt atttgggccg gtgcgaagcc ggagatttgg cgtttcgctg ggtatcaatt
    24901 tgttaccgct cgattcaaaa ctttgttcct acgattgtat ctattgtgaa tgcgggctca
    24961 ccgaacatgc agccggccac aagccaaagc ttttttcggg cgcggaaatc aggaattcgc
    25021 tgcaacagcg ttttgaagcg ctttcgcagg aatctcttgc tcccgataat atcacgtttg
    25081 caggaaacgg ggaacccacg ctgcatcccg aatttgccgc catcatcgat gacaccctcg
    25141 cgctgaggga tcaatacttt ccggaagcaa agatcaccgt cctttccaat gccaccatgc
    25201 tcaaccgccc ggcggtggtc gaagcgctga aaaaaatcga caacaacgtg ctcaagctgg
    25261 atgccggtac cgatgaaact taccgggcca taaaccgtcc attgagcccg gttacactgg
    25321 agcaggtggt ggaccggctg gccgctttcg aaggggatct gaccattcaa accttgtttg
    25381 tccggggttc ggtcgacggc cggatgatcg acaataccac agaaaaggaa gtcagccttt
    25441 ggctcggcca ccttcagcgg attcgtccgg ccctggtgat gctttatccc atcgaccggc
    25501 ggacaccgta tgcttcgcta gaaaaagtaa gtgaagccga actgcttgcc ctggcctcaa
    25561 aagtggaatc cattggcttt aaatcggaag tgttttattg atttcaggat acggtaaatt
    25621 catcagaatt ttctgaaatt agttgtttct cctttccctt cttctttgaa aatatccgta
    25681 actttacaaa aatgtagcgg acatgctgga gtatgaaccg aaaatactgg tcgcttttgc
    25741 cgaaaccctg agcgggaata ccgatatatt caactggttg ctcaataacg gttatccgga
    25801 gctggctgcc ttgtcggcaa ccttgcgcgg cagcgaagaa gcctccggct ggctcatgaa
    25861 gaacggatac cagcattttg ccgctctcga ccaggccatc gacgggaaaa tggaggctta
    25921 tttgtggttg gtgcgcaacg actaccgttt gctagccacc cttgccgatg cctgtaacaa
    25981 caagcctgaa gccattcgct ggttagccgc aaacgacctg catattttta tcatgatcgc
    26041 cgaaaaattc aggggattcc gcgacagcca gacctttgat tatcataaaa ttcatttctg
    26101 aggatgaaca atcggcaaaa tcggatgtta aaagcaattc ctaaaatatt tattaatacc
    26161 aaaaccaatg actatggaaa gtatcaaagg cacacaaact gaaaagaacc tgctgaaatc
    26221 gttcgcagga gagtcacagg cccggaaccg ctatatgttc tttgccaaaa aggcccgtga
    26281 ggaaggttat gaacagatag ccgctttttt tgaagaaacg gcccgccagg aagagacgca
    26341 tgccaagact ttcttcaaat ttttagaagg aggaatggta gagatcacgg cctcttatcc
    26401 ggccggagtt atcgggacaa ccgccgaaaa ccttaaagca gccgccgaag gtgaaaatga
    26461 agaatggact accctttaca gggaatttgg ggaaattgcc gaaaaagagg ggttcaggaa
    26521 aattgctgtt gcctataaac tgattgctac cgtcgagaaa gaacatgagg cccgttacct
    26581 gaaactcctg aagaatattg aggaagggaa agtgtttgaa agtgaggaag agaccgtttg
    26641 ggtatgccgt aaatgcgggt atcaccataa gggaaagaaa ccgccgaaga actgtccgtg
    26701 ctgcgaacat cccgaagcat actttgaaag ggaagctgta aattattaat cgggaagccg
    26761 taaggctgcc tatttagcta ttcaaattgt ccggggcttt ccgctccgga ctttttttta
    26821 aggtatttcc aggattagaa atttctgccc ccaacggagt aatggaatta atgatggata
    26881 ttgatagtaa taattaaaat aatataagtg ttaatagaaa ggcaagaatt aaggataaaa
    26941 atattagtaa ataaatcttg ttttttatcg cggctaatat taaaactatt aagcttccca
    27001 cgatgatacc cgttctcaat ttttctggtt cgatataacg ctttaagtca atagtaaaat
    27061 ataatataat gattatacaa aatattgtaa aatttaaaat ctttaagggc aaattattac
    27121 cgattttcat agcccaccaa cgtaattaga attaattgaa taaaacatta ttccaccttg
    27181 tacataaaac tggtaactaa cattactacc actgatccca ttaccccggt accgcgcgat
    27241 tcctttcacg tgttccaaca aatccacccc ttcgctcgct cgcg
//
LOCUS       DAOPYV010000041        26393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_1823_length_26393_cov_12.027755, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000041 DAOPYV010000000
VERSION     DAOPYV010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 26393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26393
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1823_length_26393_cov_12.027755"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..854)
                     /gene="lipB"
                     /locus_tag="PKI35_06945"
     CDS             complement(159..854)
                     /gene="lipB"
                     /locus_tag="PKI35_06945"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNX44157.1"
                     /translation="MKVNFLDLGIRSYQPTWDYQEKLMQEIISQKIALPREGPEGHYT
                     DNYLLFVEHPHVFTLGKSGSEANLLVNYLQLQAKNAEFIHTNRGGDITYHGPGQIVGY
                     PILNLDYFAKGVREYIRKLEESIILTLKNYGIAGERLEGATGVWIDTGFPGRTRKICA
                     IGVRVSRGVSMHGFAFNVNTDLSYFNMINPCGFTDKKVTSLQAESGREMDFKEVKEVL
                     KMNIAGVFGMSFS"
     gene            complement(1119..1988)
                     /gene="lipA"
                     /locus_tag="PKI35_06950"
     CDS             complement(1119..1988)
                     /gene="lipA"
                     /locus_tag="PKI35_06950"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNX44158.1"
                     /translation="MSDEKKDVLRKPEWLKIKLPKGENYLKVKGILEEKGLHTICSSG
                     KCPNMGECWNAGTATFMILGDICTRSCKFCATRTGKPLPADEEEPEKIAQSIKHLGLK
                     HVVITSVDRDDLPDGGACLWAATIRRVKEVNPGTTIEVLIPDFGGNQALLQVVIDAAP
                     EIISHNLETVRRLTPQVRSAAQYDRSLQVLKQIADSGIPAKSGIMAGLGESEAEVLAT
                     MDDLRAVNCSIFTVGQYLRPTPLHLPVVEYIHPDQFEKYRVAGLAKGFTHVESKPLVR
                     SSFHAEKHVQPKI"
     gene            2217..3197
                     /locus_tag="PKI35_06955"
     CDS             2217..3197
                     /locus_tag="PKI35_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HNX44159.1"
                     /translation="MNTLYPLKFAPVFKEKIWGGRKVRTLFGQDFTPLRNCGESWVLS
                     GYPGNVSVVSNGFLKGNDLNELIEVYMSDLVGDKVFQQFGNEFPLLIKLIDANDWLSI
                     QVHPDDQMARKKGFPNGKTEMWYVVQADEGAEIISGFNRDITREEYLHCMENKTLKSI
                     LNVEKAKGGDVFFMPAGRVHAMGPGLLIAEIQQSSDLTYRIYDFDRVDDKGMMRELHT
                     DLALDAADFKHYDSYRTAYDKVFNKTVNLVNAEYFSTSLIHFGTPVVKDYSAVDSFVA
                     LFCVEGSCVIGYEDGSEILKGGEVALVPAGSENITLIPQGEARILEIYVP"
     gene            3262..3786
                     /locus_tag="PKI35_06960"
     CDS             3262..3786
                     /locus_tag="PKI35_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX44160.1"
                     /translation="MKKLLVLTLIFFAGAHLMAQEELKPQFKIPSVNITDVDGNKINT
                     AELNNGGKPYIISFWATWCKPCIKELTTIADVYQDWQDETGIKLFAVSIDNSRSVAQV
                     KPLVNGKNWDYKVLLDVNNEFKRAMNVNAVPHTFLVNSKGEVVWQHTSFSEGAELELI
                     KLVRELVKTEETSK"
     gene            3820..5502
                     /locus_tag="PKI35_06965"
     CDS             3820..5502
                     /locus_tag="PKI35_06965"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HNX44161.1"
                     /translation="MSKNQILRITGILALLLLSVPVFVSAQNFLSGGQVNGNFQMDAQ
                     FYRTDSAIQAYSVPEKMMMNSWTNLTYNLGNFNAGMRFETYLNPVNGYDPRYKGAGVP
                     YWFADYKSDQFQVTAGNIYEQFGSGMAFRTYEEHNLGYDNSLRGVRVKFTPVDGVEFK
                     GIWGTQRYFWDQGAGIVRGGDVDFNLNRVIRHFESSKLKVQLGGSFVSKYQADETITD
                     NDGNELILPLNVGITAGRVNLGYDNFSLATEYAYKINDPSALNNYIYRPGQSLLVTAT
                     YSKKGLGMMLQAKRIDNMSYKSKRTETGNVLDINYLPAITRQHTYSLAAMYPYATQPN
                     GEMGVQGQVNYKIKKGTWLGGHYGTDIALNFSAIASPKYEMIDTIAISTPGTKGYESP
                     FFAIGDELYYRDINLEIGHKFSKKFKGILTLMHEDYNIEVIEGHPGEEMVHANIAIAD
                     LTYKFTDTHSLRGEFQVLQTKQDDGSWLTGLLEYTIAPKWFFSVTDMYNYGNDDSDNR
                     LHYPLVAVAYNNNANRIQVSYGKQREGIVCVGGVCRNVPASNGFTISITSSF"
     gene            5528..6436
                     /locus_tag="PKI35_06970"
     CDS             5528..6436
                     /locus_tag="PKI35_06970"
                     /inference="COORDINATES: protein motif:HMM:NF033782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28 family outer membrane lipoprotein"
                     /protein_id="HNX44162.1"
                     /translation="MKKILYTLPITLLLTGISWMFSSCDKIDEPYVVKKEVVDTTQCP
                     VPEFPAVTNPVKRALLEDYTGHSCVNCPRAAVTAHELMQQYGNSLVVVAVHAGFFAKP
                     ANAPLNYDFRTTAGTDWDDFFGIGKVGNPNGMVDRAGYPSEYILSPSAWGNAIANELG
                     KSPVAELQLMTDYDPATTKLCIHTKTSFLQSISNKAVKLSVIIVEDSIVAAQKNNDAE
                     VGATPLIEDYVHMHVMRGAVNGSWGDQILASSCLSGDPVIKSYQTSLNGFNANPIDAE
                     HCRVIAFLYDAATYEVLQVTEKAITE"
     gene            complement(6812..10636)
                     /locus_tag="PKI35_06975"
     CDS             complement(6812..10636)
                     /locus_tag="PKI35_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNX44163.1"
                     /translation="MRNLYLTLVLTVFCFTAFGTGWINIRSQQPASAKIELVSSDIST
                     SVVHFSLDGFYLSSVKTQQGEENIVTLGNSTPMLLKGTPDLPKLTASLIIPDQEIMDV
                     EVISSSYTDFANISIAPSKGNLKRDTDPSKVAYEYGRPYSENIFFPSRQAELRDPYIL
                     RDYRGQTVVAYPFSYNPVTKVLRVYTDITVKVYSKGQHGFNAITRSEMPARVDNDFKS
                     VYSRRFLNIPNTDNTPLGEYGRMLVICYGSFMEAMQPFVDWKNQMGIQTEMVDIATIG
                     NNAEAIKAYVTDYYNNHGLTFLLLVGDAAQIVTNTAGSIGGPSDNAYGYILGNDHYPD
                     IIVGRFSAETVDHVNTMVQRTLEYEKSPDISADWFSQGIGIGSDQGPGDDNEYDWEHI
                     RNIRTKLMGYTYTAVAELYDGNQGGEDQPGNPTTAMVASFVNPGSGIINYTGHGSDQS
                     WGTTGFSNNNVNQLTNNHRWPFIWSVACVNGNFPGGTCFAEAWLRAKNADGPTGAIAT
                     LMSTINQSWNPPMEGEDEMDAILVETYPDNLKRTFGGLSVNGMYKMNDTYGSDGAEMT
                     DTWTVFGDPSVNVYTAMPTNITATHESQIFIGSTGFTVEADAEGALVALSKDSQLITA
                     GFISGGTLTLEFPALVDPDTVDLVITGFNRLTYTARIPVIPNAGPYLVYNANTISDPA
                     FNNNGRADFGETVNLSLALRNIGVLDAENVNITLSTGDEYITLSDSTELCAVIPASDT
                     VNVVDGFTFTLSDNVPDLHTIAFHYTAASDTNVWSGNFNITAHAGILEFGSISINDAM
                     GNNNGIADPGETFELVLSIANTGSAEIKGTSGQISFNDPYLALLSANTQYYDTIQAGL
                     TVAKTFTVQAGINTPAGYVVPCAFAMSADQGLASSASFSVVIGQIPVAVIDLDKNFNS
                     GPKILESLQANNVYAEYLLSMPSDLSGYKALFVCLGTGTKKHVVTSAEGQLLADFVTA
                     GGKLYMEGADVWYYDPKTAVHPLFMINGIMDGANDLNKEIGQVSSFTEGLTLNYSGDN
                     EFIDHIAPTGTAFTIYKNESPYFITAVAYDGGTYKTIGSAFEFGGLVNGELPATRDEY
                     MLRIIQFFGILASPLNANFIAQPSTICDGSTASFADFSTGNVTTWEWSFPGGEPATST
                     EQNPEVYYANAGSYDVTLIVGNGITTDTLTKDKYMLVEYCTGVPESRNAEVSIYPNPA
                     GTSATLSFEGMSGTVSLRITDALSKTVLNLANIQADSSKTIDVKGMPEGIYFVIITNN
                     NKQFIKKLIVTR"
     gene            complement(10841..14761)
                     /locus_tag="PKI35_06980"
     CDS             complement(10841..14761)
                     /locus_tag="PKI35_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNX44164.1"
                     /translation="MKNFTFMRSMILSLLVVLALFSSGFAANEIQLEPGATNRLSVNE
                     SSYYRLQVTNTLNDLKFLDISTSKGLFAEMMVSGYSKSNTVGDPELPVLSKLIEIPAD
                     AVPEVKVISYDVKDYSLADWGIVSKLFPAQPPQPKNREAGDIVFNASTYSIDQFIGGE
                     LAAVSVNGYMRGVRLANLVISPVQYNPVTNTLRIYNNIVVDVRFTGANVQKTTDIRAR
                     YDSPYFRSIYNSVINLQPVERSRDTLSKMPVKYVIVSDPMFQSQLQPFIQWKTKKGFK
                     VVEAYTNDPAVGNSFNSIKAYLQNLYTSATATDPAPTFVLLVGDVAQIPAYNCGAHVS
                     DLYYCEYSGDYLPEVYYGRFSATNASELQPQIDKTLEYEQYLMPDPSFLGECVMVAGA
                     DATHGPIYGNGQINYGTTYYFNEAHGLLSHTYLQPEPSGAGYAQSIHADVSAGVAYAN
                     YTAHGSPSGWADPSFSINDVANLTNAHKYPLMVGNCCQSSTYNENCFGEALLRAENKG
                     AVGYIGASDYSYWDEDYFWGVGSGQIVVNPTYEDHGLGAYDRTFHDHGEPRSEWYSTQ
                     DQMVFAGNLAVEESNSGMKEYYWQTYCLMGDPSLMIYYSVPPVLEASYTPLLPLGTSE
                     FEVQTEPYAYIALTRDNIIHGVAEADSNGKAIMNITPFTEPCYASVVVTKQNMQPFID
                     SVLVASPAGPYLMLQNYQINDEGGNNNQLPEFGEPLDLNLTVVNYGNASAVNTLATLS
                     TSDPYLTITQPSFTWPLIPSQQSDTVSNVFGIQVAGYLPDMHQANMILKFDADTCEFT
                     YQLPVTLLAPKLQADNITISDVAGGNGNGIIEPGETIEVTVHTDNAGHCASSDVIAQM
                     IYFGNYISGSSQQQNLGPIAAGGSVESTYTFSVSPSATLGYEFNVFYVANAGAYNAIS
                     NLALTVGQQVEDFESGSFTKYNWKFAGSKPWTISNTVKYEGSYSAMSGSISNSEQSEM
                     YLNVNIISPGSITFYRKVSSEQGYDFLRFYIDDQMMGEWSGNLDWSQVSYPVETGPHK
                     LRWVYEKDEASIAYQDAAWIDYIMLPPFSETQGGPLSVYTTAVPGTICQGASSQLYVF
                     ATGGWGTYTYDWAPASALSDPSIFNPVATPSGYTLFSVNITSGTYSMTDYVEVFVEPA
                     PATPVVSVSGDHLVSTGASSYQWYDGNGIIPGATEQTYYPSHTNFYYVVTGDASGCTS
                     EPSNEVYFGFTGTSAEASAGILVSPNPFTSQLKINYVSDGSSKVKVALYNALGSEVAV
                     ISNSTMHSGTNTLYWDGSNLPAGIYTCKVFEGSKTTVTKVIHTK"
     gene            complement(14861..16090)
                     /locus_tag="PKI35_06985"
     CDS             complement(14861..16090)
                     /locus_tag="PKI35_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNX44165.1"
                     /translation="MNFPFYIARRYLVSKKSHNIINIISGISVLGVTVGTMALIIVLS
                     VFNGFEGVVVSLFNAFDPDLKITPVSGKTFRMSDLPEDSISRLPGVVHLTEVIEENAL
                     LRYHDRQCIATIKGMGNGYQDYSPLDTMLIEGQFVLERDSSEYAVPGAGIADLLGVDL
                     LDPGSMISVYVPNRHSSFTGLPDESFNTGVIRPSGIFSISAEFDIKYMLTPVSFARNL
                     FGYSEEVTSAEVSLSPQADRKLIQKQVSALAGNKFVVKNRFEQQETLYKIMKSEKWAV
                     FLILAFILFIATFNVIGSLTMLILDKRRDIAVLRSMGASDRLVRRIFLTEGLLISLSG
                     AVAGLVIGATVCWIQQRFGIIKLQGGSGAFIIDAYPVKMEACDFLWVFITVFLIGLAA
                     TWYPVRKIARKFSDQKL"
     gene            16425..18062
                     /locus_tag="PKI35_06990"
     CDS             16425..18062
                     /locus_tag="PKI35_06990"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HNX44166.1"
                     /translation="MKNLLAFSLLLGILLTGNILYAQGMEYFTNFPETGSTYNSGTFA
                     GSDGSLWTYANCRGDNPILSPSPTLGQNTSPSAFLQSGILHNGCSLLKFSYKQASAGN
                     VNLHVFINDSLAGTVTTSGQQNYVNDFWVGVHVEGDFVITIRQADSVSSGEVTIDNLS
                     WDELYTHINMEEPQFYPENFVAVAGGMSVTSTWNDVEGSGMIMAYANLLKISTDSLMV
                     PPVDSIPEGDDLDLNDGTGTVNVLFGNEEFTFENLEPATTYYLKIYPYAYPSGGGNYP
                     LYKTNGFVPSAKVFTQAPLVFYPFDNDLSPWTQYSVTGEQVWTYAAGQGINNSGCILM
                     SGYSSGNLANQDWLISPSIDLSHALEPKLRFYTALDSSNGNLPVSVFISTDYTGGDPS
                     ASGTWTELSQDLSFSEGSMAWTPSGYIDISSFAQPDVHIAFRYNSTAQAAPVWAVDDV
                     LVSFTSGVGIEEPAEEPLTFKIYPNPCEETFSLNLMEKGNYLVRILDITGKKVISRYT
                     HEGITAFNMKSMAPGIYLVIISNSGTGETAVSKVIVR"
     gene            18421..20238
                     /locus_tag="PKI35_06995"
     CDS             18421..20238
                     /locus_tag="PKI35_06995"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HNX44167.1"
                     /translation="MPFVIGALLVLVLAYRFYYSFVAAKLATISDSKLMPSQRLYDGQ
                     NYYPMNKWVLFGHHFAAIAGAGPLVGPVLAAQFGFMPGFIWMLAGAVLAGAMHDLVIL
                     VASINHDGKSLAEIARTEISKVSGATASFAILLILIVAMAGLGLVVVNALAESSWGTF
                     TIAMTIPIALLMGVWMFKIRKGKTVEATIAGVILLSAAVIFGNNIAHSGAASLFLFDH
                     KSLTLLLVAYGFIASVLPVWLLLSPRDYLSSIMKLSVVGLLAIGIIIVAPTIHMPAFT
                     PFTHGGGPIIPGKLFPYLFITIACGAISGFHALVSSGTTPKMIMSMKEVKFIAAGAML
                     SEGMVSILALIAATSLLPLDYFQINVPVEKFQAILPQLQAMGFTESNLNQLSAEVGEK
                     IAGRTGGAVSLGVGMAYIFSSIPGLQHLMSYWYHFAIMFEALFILTTIDAGTRIGRFI
                     LQETLGKIYKPFGRTNWLPGNLLASGLIVLAWGYFIYTGSVSTIWPMFGTANQLLATV
                     ALAIGTSFIINRGLARYSWMTIIPMVFVGVTTLSAAYMNMTNIYLPQIHDPVSRVQGI
                     ISFTLTAIIILCVLVVFIDAIPKWIAVMRGKRAVVTVKA"
     gene            complement(20404..21210)
                     /locus_tag="PKI35_07000"
     CDS             complement(20404..21210)
                     /locus_tag="PKI35_07000"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX44168.1"
                     /translation="MQYDPIKRSLGTVFNRTPRLRKMFYHLLDLLLLRSWHIRRNLRQ
                     WSKLAPANAAILDAGMGFGQYSYFLSGMKAGYTVKGVDVKQEQIDDCNAFFKRIGRNN
                     ASFSYADLTAFSEPLAYDLILSVDVMEHITEDVKVFENFHASLRPGGMVLISTPSDQG
                     GSDVHDHDEESFIGEHVREGYNIGEIQEKLKKAGFSRTEAKYQYGKPGQLSWKLSMKY
                     PILMLGKSKLFFIILPLYYLITFPISLILNRADVTGDHKSGTGLVVKAWK"
     gene            complement(21359..21697)
                     /gene="rbfA"
                     /locus_tag="PKI35_07005"
     CDS             complement(21359..21697)
                     /gene="rbfA"
                     /locus_tag="PKI35_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNX44169.1"
                     /translation="METTRQQKIARLIQKDLGEIFQVMCRDLFPGNMITVTKVNISPD
                     LYVAKSYLSLFGKTGKKELLDEINTRKKEIRLKLGNRIRNQVRSIPELEFFIDDSLDY
                     IENIENLLKS"
     gene            21857..23677
                     /locus_tag="PKI35_07010"
     CDS             21857..23677
                     /locus_tag="PKI35_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX44170.1"
                     /translation="MKKLSTLLRYAYPYKWHALLNIVFNILGAIFSLVSLASLVPVLN
                     ILFKQSEPVSGLMPWALDTKVVFNNLNFYISQEITRSGEINTLLVICIGIVILFLLKN
                     FFRYMALFYVAVLRNGVVKDLRNSLYHKILILPLAYYSDQRKGDIIARITSDVQEVEW
                     SILSSLEMLFRDPITIIIYLIAMFKISPQLTLFVLAVLPVAAYLIGLIGRSLRRTSQK
                     GQKKMGELLSLIEETISGLRIIKGFNAIDYSYRNFDENNRGFMRLMVRLFRKRDLASP
                     VSEFLGIAVTALILWYGGRLILSSEASLTAPKFILYIGIFSQMIVPAKAITTAFYNVE
                     KGSASIQRINQVLEAGEVIEEKPDALAVDEFRDKIEYRDVSFRYDKDFVLNNIKLTIP
                     KGKMVALVGPSGGGKSTMVDLLPRFYDVTEGGIFIDGKDLRDLRIDNLRGLMGIVNQE
                     TILFNGTIADNIAFGMKDARMEDIVHAAKVANAHEFIERLENGYNTNIGDRGIKLSGG
                     QRQRISIARAVLKNPPIMILDEATSALDTESERLVQEALLNLMKNRTSIVIAHRLSTI
                     QHADEIVVINEGKITEQGTHNELLAANGIYSRLCEMQSFS"
     gene            23854..24126
                     /locus_tag="PKI35_07015"
     CDS             23854..24126
                     /locus_tag="PKI35_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44171.1"
                     /translation="MEKRIGTITILIKGTVSVPPVNSLLSEYGSIILARQGLPMHHRN
                     IHVISLIIEGSVEVINALTGKIGRLKDVEVKSVLTKYKEEQNNEQE"
     gene            24113..25519
                     /gene="hydG"
                     /locus_tag="PKI35_07020"
     CDS             24113..25519
                     /gene="hydG"
                     /locus_tag="PKI35_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNX44172.1"
                     /translation="MNKNERNKIFIDRDKLYSLIEGKVPGESEVNDILNKALKLKGLN
                     LEDVAALLRIEDPRLIHKIMETAKTVKEEIYGKRLVLFAPVYTGNACINNCTYCSFRR
                     DNKLIKRKVLSMDEIEEDTRALLREGHKRILLICGESKRNGTDYMVEAIRRVYSIREH
                     DGRDYIRRINVELAPMEVEDFARLKAEKIGTYVCFQETYDPVLYPKFHPADTQKGNYE
                     YRLTVMDRAMEGGINDIGIGALLGLIDYRFEVLAMIEHANHLERAFGCGPHTVSVPRI
                     EYAIGAPHSENIPSKVSDEDFKKIVSIIRIALPYTGIILSTRENDTMRNELIKYGVSQ
                     LSAGSRVNPGSYAHEDQHTGSQFTLGDHRSLDEVISSLASESYIPSFCTGCYRKGRVG
                     QDFMDLAKPGLIKQYCLPNAMFTFTEYLEDFASPETKEKGLRLIEKLVGEVEKKPLQE
                     KISGNISSIRNGQRDIYF"
     gene            complement(25602..26144)
                     /locus_tag="PKI35_07025"
     CDS             complement(25602..26144)
                     /locus_tag="PKI35_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HNX44173.1"
                     /translation="MDEKKGTSYIRPTWDEYFMEISRTVAKRATCDRGRSGCVIARNK
                     QILVTGYVGSPVGLPHCDDVGHLFKQVTHADGHVTNHCMRTVHAEQNAICQAARLGIP
                     LEGSTLYCRMTPCRTCAMLIINCGIKRVVCEFKYHSGSESEEMFAQAGIDLSYVNPEV
                     LQYARQQADDAAPDDEETGS"
     gene            complement(26153..>26393)
                     /locus_tag="PKI35_07030"
     CDS             complement(26153..>26393)
                     /locus_tag="PKI35_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HNX44174.1"
                     /translation="FSTNVVAYDMSALMKYKGFPLEDAAGEVVMKKLKAQGGTGGVIA
                     VDKNGNIAMPFNTAGMFRGYVKEGGEMKVALFGDQ"
BASE COUNT         6842 a         6303 c         6391 g         6857 t
ORIGIN      
        1 gcctgcctgc ctgggctggc tgtgcgtaag atggcaggcc aacagccaaa gataaaaagt
       61 aaaaggttgt gagggtgaaa gctgtgagta aaaagccggg atcctaagtc cggatgtctg
      121 aattctctga tagatcatgg gcttacttca atcataagtt aactgaaact cattccaaag
      181 acgccggcaa tattcatttt taacacctct tttacctctt taaaatccat ttccctgccc
      241 gattctgcct gcagcgaagt tacttttttg tcggtgaagc cacaaggatt gatcatgtta
      301 aagtaagaaa ggtcggtatt cacgttgaag gcgaaaccat gcatgctcac acccctgcta
      361 acgcgaaccc cgatagcgca gatttttcgt gtcctgccgg ggaaaccggt atcgatccat
      421 acccctgtgg ccccttcgag ccgttcgcct gcaatgccgt agtttttcag ggtaagaata
      481 atggattctt caagtttccg gatgtattca cgcacgcctt tggcaaaata atcaaggttc
      541 aggatggggt atcccacaat ttgtcccgga ccatgatagg tgatatcgcc gccgcggttg
      601 gtgtggatga attcggcatt ctttgcctgt agttgcaggt aattcacaag caggttggct
      661 tcggaaccgc ttttacctaa cgtaaacaca tggggatgct ctacaaacag cagatagtta
      721 tcggtgtaat ggccttccgg gccttccctg ggaagagcaa tcttctggct gatgatttcc
      781 tgcatgagct tctcctgata atcccaggtt ggctggtacg aacggattcc aaggtcgagg
      841 aagtttactt tcatttagaa ttttttttca acagtgaccg gcaaataaat cagtagtttt
      901 aattaaaagc cttacaggcg ggcgtttcag cgtcattcct gaggtgaagc ccctcgcctt
      961 taggagaggg gttggggtga ggttttttgg gaggaagtcc atgtattaat ccgttctgct
     1021 ggtcggaaat aacttttttt gtataaacaa tctctattag taactaacag aacctcagcg
     1081 ctccgaatcc tccgggtcag cataataatt ttcagctatt aaatttttgg ctgaacatgc
     1141 ttttccgcat gaaaactgct tcgtaccagc ggtttgcttt ccacgtgggt gaagcctttc
     1201 gccaacccag caacacggta tttctcaaat tggtcgggat ggatatattc cacaacggga
     1261 agatgcaagg gagtaggccg gaggtactgc ccgacagtaa agatgctgca attcacggcc
     1321 cgcaggtcgt ccatggttgc cagcacttcg gcttcacttt cgcccagtcc cgccatgatt
     1381 cccgatttgg ccggtatgcc cgagtcggca atctgtttca acacctgcag gcttctgtcg
     1441 tattgagcgg cgctgcgcac ctgcggcgta agcctgcgta ctgtttcgag gttgtgcgag
     1501 atgatctccg gggcggcatc aatcaccacc tgcaggaggg cttgattccc tccgaaatca
     1561 gggatcagca cttcaatggt ggtccccgga ttcacttcct tcaccctccg gatggtagcc
     1621 gcccacaggc aagcgcctcc atcgggaagg tcgtcgcggt ctaccgaagt gatcaccacg
     1681 tgttttaatc ccaggtgctt tatcgattgg gcaattttct ccggctcctc ttcatctgcc
     1741 ggaagaggct ttcctgtcct ggtagcgcaa aatttacagc ttcgtgtgca gatatcaccg
     1801 aggatcatga aggtagcggt ccccgcattc caacactccc ccatattggg acatttgccg
     1861 ctggaacaga tcgtgtgaag ccctttttcc tccagtattc cttttacttt taaataattt
     1921 tcacctttag gaagttttat tttgagccat tccggtttgc ggagaacatc ctttttttcg
     1981 tctgacatag caacataagt ggttatataa ataactaact gtgaacatat taagattccg
     2041 gaatatgatg actaaccacc cggaaattgt tggcaaaagt acggtttttc agcctgtttc
     2101 accgctttaa caaatatttg gcagtggagg ccgtaatgaa taaagttgta tttttgaagc
     2161 attgcaaaca ttcaattttc ttcataagcg atactaatcg tatctcagga ttacggatga
     2221 atacacttta cccgcttaaa ttcgcccccg ttttcaaaga aaaaatatgg ggaggccgga
     2281 aagtcaggac actgttcgga caggatttta ctcctttacg gaattgcggt gagtcctggg
     2341 ttctttcagg atatcccggt aatgttagcg ttgtatcgaa cggctttctt aaagggaacg
     2401 acctgaacga gctgatagag gtctatatga gcgatcttgt cggcgataag gtgttccagc
     2461 agttcggtaa cgaatttccc ttgctcatca aattgataga tgccaacgac tggctttcaa
     2521 tacaagtcca tcctgatgac cagatggccc ggaaaaaggg ctttccgaac ggcaaaaccg
     2581 agatgtggta cgttgtacag gccgatgagg gggctgaaat catttcaggt ttcaaccggg
     2641 atataacccg cgaagaatac ctgcattgca tggaaaacaa aaccctgaaa tcaatcctga
     2701 atgttgaaaa ggctaagggc ggggatgttt tcttcatgcc tgccggacgg gttcatgcca
     2761 tggggcccgg attgctgatt gccgaaattc agcaaagcag cgacctgaca tacaggattt
     2821 atgactttga ccgcgtggac gataagggca tgatgcgtga actccacacc gatctggccc
     2881 tcgatgctgc cgattttaaa cattatgaca gttaccgtac tgcatacgac aaggttttca
     2941 ataaaacggt gaacctggta aacgctgaat acttctccac cagcctgatc cattttggca
     3001 cgccggttgt aaaagattac agcgctgttg attcttttgt ggcgcttttc tgcgtagagg
     3061 gatcgtgcgt tatcggctac gaggacggtt ctgagatttt aaaaggagga gaagtagcgc
     3121 tggtacccgc cggctccgaa aacatcacgc tcattccgca gggtgaagcc cggatacttg
     3181 aaatctacgt accctgatac caggaatcgc cggttagaat tgtctgttca ttataataaa
     3241 ccaagataaa caaacccaat catgaaaaaa ctactggtcc tgactctaat tttctttgca
     3301 ggggcacacc ttatggccca ggaagaacta aaaccgcagt tcaaaattcc ttccgttaac
     3361 attaccgatg ttgacggcaa caaaataaat accgccgaac ttaacaatgg cggcaaaccc
     3421 tatattatca gcttctgggc cacctggtgc aagccttgca tcaaagaact cactactatc
     3481 gccgatgttt accaggactg gcaggacgaa accggcataa aactgttcgc tgtatctatc
     3541 gataattcac gttcggtggc ccaggtaaag cccctggtaa acggtaaaaa ctgggattac
     3601 aaggtgcttc ttgatgttaa taatgaattc aaacgcgcta tgaacgtaaa tgccgttccc
     3661 cataccttcc tggtaaacag caaaggcgaa gtggtgtggc agcataccag tttcagtgaa
     3721 ggcgccgaac tggagcttat caaactcgta cgggaacttg ttaaaacaga agaaacttca
     3781 aaataacacg atgtcgatca attcagataa agaaaattta tgtcgaagaa ccagatactc
     3841 cgtataaccg gtatactggc actgttatta ctatcagtgc cggtttttgt ttctgcccag
     3901 aactttctct ccggtggaca ggtaaacggg aatttccaga tggatgccca gttttaccgg
     3961 accgattcag ccattcaggc atactcggtt cctgaaaaaa tgatgatgaa cagttggacc
     4021 aacctgactt acaaccttgg caatttcaat gcagggatgc gtttcgaaac ttacctcaac
     4081 ccggtgaacg gatatgaccc tcgttacaaa ggagccggcg ttccctattg gtttgccgat
     4141 tacaagtccg accagttcca ggttacagca ggcaatattt acgaacaatt cggatcggga
     4201 atggcatttc gtacttatga agaacataac ctgggctatg acaactcatt gcgcggcgtg
     4261 agggtgaaat tcacccctgt tgatggtgtt gaatttaaag gtatctgggg tacgcaacgt
     4321 tacttctggg accagggagc aggaatcgta cgcggcggcg atgtggattt caacctgaac
     4381 cgcgtcatca ggcattttga aagcagcaaa ctgaaagtac agttgggcgg cagttttgta
     4441 agcaaatacc aggccgacga gaccatcacc gacaatgacg gcaatgagtt gatactgccg
     4501 ctaaacgtgg gtataaccgc tgggcgcgta aacctgggat acgataattt tagccttgca
     4561 acggaatatg cctataagat caacgaccca tcggcgctca acaattatat ttatcgtccc
     4621 gggcagtcac tgctcgttac agctacctat tcaaaaaaag gactgggaat gatgttacag
     4681 gcaaaacgga ttgataacat gagttacaaa tcgaaacgta ccgaaaccgg caatgtactc
     4741 gatatcaact acctgcctgc cattacacgg caacatactt attccctggc cgccatgtac
     4801 ccttacgcaa cccagcccaa tggcgaaatg ggggttcagg gacaggtcaa ctacaaaata
     4861 aagaaaggaa catggcttgg gggtcattat gggacggata ttgccctgaa tttctcggcc
     4921 atagcttctc ccaaatacga aatgatcgat acgatagcta tctcaactcc gggaacaaaa
     4981 ggttatgaat ccccgttctt cgccattggt gacgaattat attaccggga tatcaacctg
     5041 gagataggcc acaaattcag caaaaaattt aaaggtattt taaccctgat gcatgaggac
     5101 tataatattg aagtgatcga aggccatccg ggtgaagaaa tggttcatgc caatatagcc
     5161 attgctgatt taacctataa attcaccgac acccattcgc tccgcggaga gttccaggtg
     5221 ctccagacca aacaggacga tggcagttgg cttaccggct tactcgaata taccattgcc
     5281 ccgaagtggt tcttcagtgt gacggatatg tacaactacg ggaatgatga cagtgataac
     5341 cggctgcact acccgttggt ggcagtggca tataacaaca atgccaaccg catccaggtt
     5401 tcctacggca agcagcgcga aggaattgtt tgcgtaggcg gagtttgccg caacgtgcct
     5461 gcatccaatg gtttcacaat atcaattacc agcagtttct gatgttgaaa catgaaaagc
     5521 caataacatg aaaaagatat tatacacttt accgataaca ctcctgctga cagggatttc
     5581 atggatgttt agttcctgcg ataaaattga tgaaccgtat gtcgtcaaaa aggaagtggt
     5641 tgacacaacg caatgtcctg ttccggaatt cccggccgtt accaatcctg tgaaacgggc
     5701 tcttcttgag gattataccg gccactcctg cgtaaactgc cccagggctg ccgttacagc
     5761 ccacgaacta atgcaacagt acggcaattc actcgttgtg gttgccgttc atgccggatt
     5821 ttttgcaaaa ccggcaaatg cgcctctcaa ttacgatttc agaacgaccg ccggtaccga
     5881 ttgggacgac ttcttcggta ttggtaaagt cggtaatcca aacggaatgg tcgacagggc
     5941 cgggtatcct tccgaataca ttttatcgcc atcagcctgg ggaaatgcca ttgccaacga
     6001 acttggcaaa tcccctgtgg ccgaattgca gttgatgacg gattacgacc ccgctacaac
     6061 aaaactatgc atccatacca aaacctcatt tctgcaaagt atcagcaata aggctgtcaa
     6121 attatcggta atcatcgtgg aagacagcat cgtggcagcc cagaaaaata atgatgcgga
     6181 ggttggcgct acgccgctca tcgaagacta tgtgcacatg cacgtgatgc ggggcgccgt
     6241 caacggttcc tggggcgatc agatactggc atcatcttgc ctttccggtg atcctgttat
     6301 aaaatcgtac cagaccagcc tcaacggatt taatgccaat ccaattgatg ctgaacactg
     6361 ccgcgtgatt gccttcctgt atgatgccgc aacctacgaa gtgctgcagg taacggaaaa
     6421 agcgattaca gaataacaac tgtaaactat taatccaaaa cccgctccgg tcaacggcag
     6481 cgggtttttt atgcggatac cagggaggaa aggcgggtat tatagaagaa gataagaaat
     6541 ggaaggacaa agatattcag attagtattt gccagccagg agaagggaaa ccgtaccgga
     6601 aagagggaaa cctgtcagcg tccgcattca gggacagacc tgacagcgcc ggataataac
     6661 agggatttgt actgcaacag ctattaaaga tagatacact gtagacttga taaaatctta
     6721 acggtaaaac acatccagcc aattaaaaac cccgctcctg taaaagggaa cggggtttta
     6781 ttatgaagcg ctttgctgat tattcctgag gttatctggt tacgatcagc ttctttatga
     6841 attgtttgtt gttattggta atgatgacaa aataaatacc ttccggcatg ccttttacat
     6901 caatggtttt ggaagaatca gcctgaatgt tggcaaggtt gaggaccgtt ttactcaacg
     6961 catcggtgat gcgaaggctg acggttcccg acattccttc gaaacttaat gttgccgagg
     7021 tgccggcggg attcggatat atgcttactt cggcgttcct gctttcggga acaccggtac
     7081 agtattcaac cagcatatac ttgtctttgg tcagtgtatc ggtggttatt ccgttaccga
     7141 cgataagcgt gacatcataa ctgccggcat tggcatagta aacttcagga ttctgttcgg
     7201 tactggttgc cggttcccct ccggggaaac tccattccca agtggtgacg tttccggttg
     7261 agaaatctgc aaatgaagct gtacttccat cacaaatagt ggaaggctgt gcaatgaagt
     7321 ttgcgttaag cggcgaagcc aggataccga agaactggat aatgcgcaac atatattcgt
     7381 cacgggttgc aggtaattcg ccattaacaa gtccgccaaa ttcaaatgcc gacccgatgg
     7441 tcttataggt tcccccatcg taagcaactg ccgttataaa ataggggctt tcatttttat
     7501 aaatagtaaa agccgtaccg gtaggcgcta tatggtcgat aaattcatta tcaccgctat
     7561 agtttaacgt caggccttcg gtaaaggaac taacctgccc gatttccttg ttcaggtcgt
     7621 tggcgccatc catgattccg ttgatcataa agagcggatg aacggcagtc tttggatcat
     7681 aataccatac gtcggcgcct tccatataca gctttccgcc ggcagttacg aaatcggcaa
     7741 gaagctgccc ttcagccgaa gtaacaacat gctttttggt gccagttccc agacagacaa
     7801 agagtgcttt ataacctgaa aggtccgaag gcatcgaaag cagatactcg gcatagacat
     7861 tgttcgcctg aagcgactcc agaatcttcg ggcctgagtt aaagttttta tcgaggtcga
     7921 tgacggctac aggtatttgt ccgatcacta ccgaaaatga ggcggatgat gcaagtccct
     7981 gatcggctga cattgcaaag gcacacggga caacatatcc tgccggtgta ttaataccgg
     8041 cttgaaccgt aaatgttttc gcaaccgtca gccctgcctg gattgtgtca taatactggg
     8101 tgttagccga cagcagggcc aggtaaggat cgttgaatga tatctggccg gatgttcctt
     8161 ttatttcagc ggaacccgta ttggcaatac ttaaaaccaa ctcgaaggtc tcacccggat
     8221 cggcaattcc gttgttgttg cccatggcat cgttgatgct gatggatccg aactcaagaa
     8281 tcccggcatg ggcggttatg ttaaagttac cgctccatac attggtatcg cttgcagccg
     8341 tataatggaa tgctatggta tgaaggtcag gaacgttgtc ggacagggta aaggtgaaac
     8401 cgtcaaccac gttgactgtg tcgctggccg gtatcacggc gcacaactct gttgaatcgg
     8461 acagggtgat atattcatca ccggtcgaaa gtgtgatatt tacattttca gcatccagta
     8521 ctcctatgtt tctgagcgct aacgacaggt ttaccgtttc gccaaaatcg gcgcggccat
     8581 tgttgttaaa tgccggatcg ctgatggtat tggcattata aaccagatag gggcctgcgt
     8641 ttggtatgac ggggattctt gcagtatagg tcaggcggtt gaaaccggta attaccaggt
     8701 caacggtatc gggatcaaca agggccggga actccagggt aagggtgcct cccgaaatga
     8761 agccggcagt gattaactgg ctatcctttg agagagcaac cagggcgcct tcagcatcgg
     8821 cttccactgt aaacccggtt gacccgataa atatctggga ttcatgcgtt gccgtaatat
     8881 tggttggcat tgccgtatag acattcaccg aaggatctcc gaaaacggtc catgtatcag
     8941 tcatctcggc gccatcgctt ccgtaagtat cgttcatctt atacattccg ttgacggata
     9001 atccgccaaa ggtgcgtttc aggttatccg gatatgtttc cacaagtatg gcatccattt
     9061 catcctctcc ttccatggga gggttccagc tttggttgat ggtcgacatc agagttgcta
     9121 tggcgcctgt agggccgtca gcgtttttag cccgtaacca ggcttcggca aaacaggtgc
     9181 cacccgggaa attgccgttt acacaggcaa cgctccatat aaatggccac ctgtgattgt
     9241 tggtgagctg gttcacattg ttgtttgaga agccggtagt tccccaggac tgatcactgc
     9301 cgtggcctgt ataattgatg atgccggaac ccgggtttac gaatgaggcc accatcgcag
     9361 tagtaggatt tccaggttga tcttcgcctc cctggtttcc gtcgtaaagt tcggctaccg
     9421 ctgtataggt atatcccatc aatttagtgc ggatattgcg gatatgctcc caatcatatt
     9481 cgttatcgtc gccgggcccc tggtccgatc cgatcccgat cccctggctg aaccagtcag
     9541 cgctgatatc cggacttttt tcatattcca gcgtgcgctg aaccatcgta ttcacgtgat
     9601 ctacggtctc agccgagaac cgccccacaa tgatatcagg ataatggtcg tttcccagga
     9661 tgtaaccgta agcgttgtcc gaagggccgc caatgctgcc ggctgtattg gtgacaatct
     9721 gtgcggcatc gcccaccaga agcaggaaag tcagcccgtg attgttgtaa taatccgtaa
     9781 cataggcttt tatagcttcg gcgttgttgc cgatggtcgc aatgtcaacc atttccgttt
     9841 gaatacccat ctggtttttc cagtcgacaa agggctgcat ggcttccata aagctgccgt
     9901 aacaaatcac aagcattctg ccatattcac ccaggggagt attatccgtg tttgggatgt
     9961 tgaggaacct gcgggaataa accgatttaa aatcattatc aacccgggca ggcatttcac
    10021 tgcgggtgat tgcgttgaaa ccatgctgcc ctttcgaata aacttttacg gttatgtcgg
    10081 tataaaccct taatactttg gtaacaggat tgtacgaaaa cggatatgcc actacggtct
    10141 gtccgcggta atcgcgaaga atatacggat cgcgcaattc agcctgccgc gacgggaaaa
    10201 aaatgttttc ggaatagggc cgtccatact cataagctac cttcgaagga tcggtatcgc
    10261 gcttgagatt tccttttgat ggcgctattg aaatatttgc gaaatcggtg taggaggatg
    10321 aaatcacttc cacatccata atttcctgat cggggataat taacgaggct gtcagtttgg
    10381 ggaggtcggg agtgcctttt aaaagcatag gcgtggagtt ccccagcgtc acaatgtttt
    10441 cttcaccttg ctgtgttttc acactactga ggtaaaaacc atcgagcgaa aaatggacaa
    10501 cagaagtgga tatgtctgac gataccagtt ctatttttgc tgatgcaggt tgttgggacc
    10561 ggatgttaat ccatcctgtt ccgaaggccg taaaacagaa tacggtcaat acaagcgtaa
    10621 ggtaaaggtt tctcatataa tcctggttat gtaatgaatg ttattaagat tcggtaaacg
    10681 acgattatga tgaaccggag agttccgggg tttaaggggc ataaagatat gataaaaaca
    10741 tttaacaatt gaaattcaac ttccttggta aaagaaaaac tgcctgtggc ggaaccttcc
    10801 ggtatccgca acaggcagtg ggtatttcgg ttttgggatg ctattttgta tgaattactt
    10861 tggtgacggt ggtttttgaa ccttcaaata ctttacaggt ataaatgcct gcaggaaggt
    10921 tgctgccatc ccagtaaagc gtgtttgttc ctgaatgcat cgtgctgttg ctgattacag
    10981 cgacttcgga gccaagtgca ttataaaggg caacctttac ttttgaggaa ccgtcagata
    11041 cataattaat tttcagttgt gatgtgaagg gatttggaga taccaggatt ccggccgacg
    11101 cctcggcaga agttccggta aaaccgaaat acacttcatt ggaaggttcg gaggtgcatc
    11161 ctgaagcatc gccagtcaca acataataga aatttgtatg tgacggataa taggtttgtt
    11221 ccgtagcacc gggaataatt ccgttaccat cgtaccattg ataactgctg gcgccggtcg
    11281 aaaccaggtg atcgcccgat accgatacga caggagtagc cggcgccggt tcaacaaaca
    11341 cttcaacata atcggtcatg gagtaagttc cgctggtgat gttgacgctg aaaagtgtat
    11401 agcccgatgg cgttgctacc ggattgaaaa tcgacggatc gctgagcgct gaagcaggag
    11461 cccagtcgta ggtataagtt ccccagcctc cggtggcaaa tacataaagc tgactcgaag
    11521 ccccctggca gatagtgccg ggaacagcag ttgtatatac cgaaagcggg ccgccctgtg
    11581 tttcggagaa cgggggaagc atgatataat caatccatgc ggcatcctgg tatgcaatgg
    11641 aggcttcatc cttttcataa acccacctta gtttgtgggg accggtttca accgggtacg
    11701 aaacctggct ccagtcaagg ttgcccgacc attcacccat catctggtca tcaatataga
    11761 acctcagaaa atcgtaaccc tgttctgagg atactttccg gtagaaggta atgctgccag
    11821 gtgagatgat attcacattc aggtacatct cgctttgttc tgaatttgaa atgctgcccg
    11881 acattgcgct gtacgaacct tcatatttca ctgtattgct gatggtccac ggttttgatc
    11941 cggcaaattt ccagttgtac ttggtaaagg aacccgattc aaagtcttcc acctgctgcc
    12001 caaccgtaag ggccaggttt gaaatagcat tataggcccc tgcattggca acataaaaca
    12061 cgttaaattc ataaccgaga gtagcgcttg gacttactga gaaggtataa gtggattcca
    12121 cggagcctcc ggcggctatc ggcccaaggt tttgctgctg agagctgcca gagatataat
    12181 tcccgaaata aatcatttgt gcaattacat ctgacgatgc gcaatgcccg gcgttatcgg
    12241 tatgaacggt tacttcgatg gtttcgcccg gttcaatgat cccgtttccg tttcctccgg
    12301 ccacgtcgct gatggttatg ttgtcggcct gcagtttagg cgcaagcaag gtcaccggca
    12361 actggtaggt gaattcacag gtatcggcat caaatttcag tatcatgttt gcctggtgca
    12421 tatcgggaag atatccggct acctgaattc cgaaaacatt gcttaccgta tcgctctgct
    12481 ggctgggaat aagcggccag gtaaaggaag gctgggttat ggtaagatag ggatcgctgg
    12541 ttgaaagcgt ggccagcgtg ttaacggcgg aagcgtttcc gtaatttaca acggttaggt
    12601 tgaggtcaag aggttcgccg aattcgggca actggttatt gtttccgcct tcgtcattga
    12661 tctggtagtt ctgaagcatc aggtacggac cggcaggtga tgcaaccaac acgctgtcga
    12721 taaacggctg catattttgt ttcgtaacca caacactggc ataacaaggc tcggtgaatg
    12781 gggtgatatt cattatcgcc ttaccattgg aatcggcttc ggcaacgcca tggataatat
    12841 tatcccgggt cagggctatg tacgcgtaag gttcggtttg tacttcaaat tccgaggtcc
    12901 ccagtggcag caatggagta taactggctt ccaaaacagg gggaaccgaa taataaatca
    12961 tcagggaagg atcgcccata aggcaatagg tctgccaata gtactctttc atgcccgaat
    13021 tcgactcttc aacggcaaga ttccctgcga aaaccatctg gtcctgagtt gaataccatt
    13081 cgctcctggg ttcgccatga tcgtggaaag tgcggtcata agccccaagc ccgtggtctt
    13141 cataggtagg attgaccaca atctgtcctg atccgacgcc ccagaaataa tcttcgtccc
    13201 aataactgta atctgatgcg ccgatatagc caacagcccc tttattttct gcgcggagaa
    13261 gggcttcacc aaaacagttt tcattgtacg ttgaagactg gcagcagttt cctaccatca
    13321 aaggatattt gtgggcattg gtaaggttgg caacatcgtt gatgctgaag gaaggatcgg
    13381 cccagccgct tggcgaaccg tgtgcggtat agttggcata agctacccct gcgcttacat
    13441 cggcatggat actttgtgca tagccggcgc ctgaaggctc aggctgaagg taggtatgtg
    13501 acagcaatcc atgggcttca ttaaaataat aggtagttcc atagttgatc tgcccgttgc
    13561 cgtaaatcgg cccgtgggta gcatcggctc cggcaaccat gacacattcg cccaggaatg
    13621 aaggatcagg catgaggtat tgttcatatt ccagggtttt gtcaatctgt ggctgaagtt
    13681 cagatgcatt ggtggccgaa aacctgccgt aataaacctc aggaaggtaa tcgccgctgt
    13741 attcacagta atataagtcg gaaacgtgcg caccgcagtt ataagcggga atctgagcca
    13801 catcgcctac cagcagaacg aaggtgggag ccggatcggt tgcagtggcc gatgtataaa
    13861 ggttctgcag gtatgccttg atggaattga aagaattgcc cacagccgga tcgttggtgt
    13921 aagcttctac tactttaaat ccttttttgg tcttccactg gatgaaaggc tgtaattggc
    13981 tctggaacat cgggtcggag acaatcacat attttacggg cattttcgac aaggtatcgc
    14041 gactgcgttc aaccggctga aggttgatga cgctgttata gatgctcctg aaatacgggg
    14101 aatcgtaacg ggcgcgtatg tcggtggttt tttgtacgtt ggctcccgtg aaacgaacat
    14161 caaccacaat gttgttatag atacggagtg tattggtaac cggattgtac tgaaccggcg
    14221 aaatcaccag gttggcaagc ctcacgcccc tcatatagcc attcacgctt acggctgcaa
    14281 gttctccccc gataaactgg tcgatcgaat aggtcgatgc attgaaaaca atgtcgccgg
    14341 cctccctgtt cttaggctga ggaggttgtg ccgggaacag tttgctgaca attccccaat
    14401 ctgccaggct ataatccttt acatcataac tgatcacttt tacttcagga actgcatcgg
    14461 caggaatttc gatgagtttg ctcaggactg gaagttccgg atcgccgact gtattcgatt
    14521 ttgaatatcc ggaaaccatc atttcggcaa acaggccttt ggaggtgctg atgtccagaa
    14581 atttcaggtc attcagggta ttggtaacct gcaacctgta ataactgctt tcattgaccg
    14641 aaagacggtt tgttgctccg ggttccagtt gtatttcatt tgcggcaaag ccggatgaaa
    14701 acaacgctag aaccacaagc agtgataaga tcatgctgcg cataaaagta aagtttttca
    14761 tgttggatta attggttgaa ggggtaggaa aatatttagc cacaaatgta aacttttaga
    14821 aaatatctgt gtaatgaaat attaaaaaat acgacatgga tcagagtttc tgatcgctga
    14881 attttcgggc gattttgcgc accggatacc aggtggctgc cagaccgatc aggaatacgg
    14941 taatgaacac ccataaaaag tcacaggctt ccatttttac cggataggcg tcaatgataa
    15001 aagcgccgct gccgccctgc agttttataa tcccgaagcg ctgttgtatc cagcataccg
    15061 tggcgccgat cacaagcccg gcaacagcgc ctgatagcga aatcaacaag ccttcggtca
    15121 ggaagatgcg gcggaccagc ctgtcgctgg cgcccatgct gcggagcacg gcaatgtcgc
    15181 gccgcttgtc gaggatgagc atggtcagcg aaccaatcac gttaaaagtt gcaatgaaca
    15241 ggataaaagc cagtatcagg aaaactgccc acttttccga tttcatgatc ttgtacaggg
    15301 tttcctgttg ctcaaaacgg tttttcacca cgaatttgtt tccggccagg gccgaaacct
    15361 gcttctggat cagtttcctg tcggcttggg gcgaaaggct tacctcggcc gaagtaacct
    15421 cctcggaata tccgaaaagg tttctcgcga acgaaaccgg cgtcagcatg tacttgatgt
    15481 caaattcggc cgatatcgaa aaaataccgg acggccgtat gacccccgta ttgaaagatt
    15541 catcaggcag gccggtaaag ctggaatgcc tattggggac atataccgaa atcatactgc
    15601 ccggatcaag taagtcaacg cccagaagat cggctatccc tgctccggga accgcatatt
    15661 cgctgctgtc gcgttcaagg acaaactggc cttcgataag catggtgtcg agcggcgaat
    15721 aatcctgata accgttcccc atccctttga tggtggcaat acattgcctg tcgtgatagc
    15781 ggagaagggc attttcctct atcacttcgg taaggtgtac tacccccggc aaccggctta
    15841 ttgaatcctc cggcagatcg ctcatgcgga aagtcttacc gctgacaggg gttatcttta
    15901 aatcaggatc aaaagcattg aacaacgaaa ccacgacacc ctcaaaaccg ttgaaaacgc
    15961 tcaggacaat aatgagcgcc atcgtcccga cggtaacgcc caataccgaa atacccgaga
    16021 tgatattaat gatattgtgc gactttttcg aaacgagata acggcgggct atgtaaaacg
    16081 ggaaattcaa aacggtgttg ttggttacgt caagggcaaa tgtaacgctt tccggtttat
    16141 gtaggcatta ccctaataaa aaggaacgaa cctgattaat gcccggaaaa aggctgaaaa
    16201 ttcaagcttt tgtacagttg gattcttaat ttctgaaaat gactttaata tattcatatt
    16261 ggaatatatg cctatatctt tatcaacaaa acatttaggg atatttcagg aattgattta
    16321 aattctaaaa ataagatgcc aatttatatc aaatggcaca aaaatttatt aattttacaa
    16381 ccggacattt ataagttacg atcggatatt taatttaatc ggccatgaaa aatttacttg
    16441 cattttcact gctgttgggc attctcttaa caggcaatat tttgtatgcc cagggaatgg
    16501 aatattttac caattttccg gaaaccggca gtacatacaa tagcgggaca ttcgcaggtt
    16561 ccgacggttc actgtggacg tatgcaaatt gccggggcga caatccgata ttatccccat
    16621 cgcccaccct ggggcaaaac acctctcctt cggcatttct gcagtcaggc atactgcaca
    16681 atggctgcag ccttttaaag tttagttaca agcaggcgtc agccgggaat gtgaacctcc
    16741 atgtttttat taatgattcc ctagcgggga ctgtcactac atcggggcag cagaattacg
    16801 tcaatgattt ttgggtaggt gttcatgtgg aaggtgattt tgtaattacg atcagacagg
    16861 ccgacagcgt atcgtcaggt gaagtcacta ttgacaacct gagctgggat gaattataca
    16921 cccatattaa catggaagag ccccagtttt atccggagaa ttttgtcgcc gttgccgggg
    16981 gaatgtccgt tacatcaaca tggaacgatg ttgaaggctc agggatgatt atggcttatg
    17041 ccaacctgct taaaataagt accgattcat tgatggttcc gcctgtcgac tccataccgg
    17101 agggcgacga cctggactta aacgacggta cgggaacagt gaatgtcctc ttcgggaacg
    17161 aagaattcac cttcgaaaac ctggaaccgg caaccaccta ttacctgaag atttacccgt
    17221 atgcttaccc gagcggtggc ggtaattatc cattatataa aaccaacggc tttgtgcctt
    17281 cagccaaggt tttcacccag gcgccgctgg tcttctatcc ttttgataat gacctgtccc
    17341 cctggaccca atacagtgtt accggtgaac aggtttggac ctatgctgcc ggtcagggaa
    17401 tcaataacag cgggtgtatc ctcatgagtg gatattcatc ggggaacctt gccaatcaag
    17461 actggctcat ttctccttcc atcgacctct cccatgccct tgaaccgaaa ctcaggtttt
    17521 acactgccct cgacagcagc aatggtaatc tcccggtatc cgtttttatc tcgacagatt
    17581 ataccggtgg tgatccttcg gccagcggca catggacgga actgagccag gatttatcct
    17641 tttctgaagg ctcgatggcc tggactccct cggggtatat cgacatatcg tcttttgccc
    17701 agcctgatgt tcacattgcc ttccggtaca actctaccgc tcaggccgct cctgtatggg
    17761 ccgttgacga tgttctggta tcgtttacat cgggagtggg aatagaagaa ccggcggaag
    17821 agcccttaac gtttaaaatt tatccgaatc cctgtgaaga aactttttcc ctgaacctta
    17881 tggaaaaagg gaactacctg gtgcgcatcc tggatatcac cggcaagaag gttatcagcc
    17941 ggtataccca tgaaggcatt acggctttca atatgaaatc gatggcgccg ggaatatatc
    18001 tggtaattat cagcaactcc ggtacaggag aaaccgccgt ttcaaaagtc attgttcggt
    18061 aaaccggctc ataatcctgt cctgaaattg ggatatagcc ggcggtgtcc ctgaataaat
    18121 tctttcatac aaggccggac cttgttttca gttggtgaaa atctgctgat atcatcattc
    18181 ttttaccgct gcatcgaaac ttttctttca ttttcccggc tgatgaaatt ttttttaaga
    18241 tgcattttct ttcaccggaa cccggctgat gctttgccaa tgctgacaat actgcccccg
    18301 gcaaacccgg gaagtgtttt cggaaggaca ataattaaaa attaaacggg aattcaggct
    18361 tccctttcat ggcagattgc taattttgaa cgttcttaca cgtactgctc tatgaatgcc
    18421 atgccttttg tgataggcgc cctgcttgtt ctcgtgcttg cttaccgttt ctattacagt
    18481 tttgtagccg caaaactggc caccatcagc gattcgaaat taatgccgtc gcaaaggctt
    18541 tacgatggcc agaactatta tcccatgaat aaatgggtat tgttcgggca tcattttgcc
    18601 gccattgccg gagctggtcc gttggtgggc cctgtgctgg cagcccagtt cggattcatg
    18661 cccggtttta tctggatgct ggcaggagcc gtgctggcag gagccatgca cgacctggtg
    18721 atcctggtgg cttcgatcaa ccatgacgga aaatcactgg ccgagatcgc ccggaccgaa
    18781 atcagcaagg taagcggggc gacggcttcc tttgccatcc tgctgatcct gatcgtggcc
    18841 atggccggtc ttggattggt agtggtaaat gcccttgccg agagttcgtg gggtaccttc
    18901 accatcgcca tgaccattcc catagccctg cttatgggag tgtggatgtt caaaataagg
    18961 aaaggcaaaa cggtggaagc taccattgcc ggtgtgatcc tgttatcggc agcggtcatt
    19021 ttcggcaata atatcgcaca ttccggtgcg gcttccctgt tcctgttcga tcataaatcg
    19081 cttaccctgc tgttggtagc atacgggttc atagcatcgg tgctgccggt atggctgttg
    19141 ctttcgcccc gcgattacct cagctccatc atgaaactca gcgtggtcgg cttactggcc
    19201 ataggcatca tcatcgtggc gccgaccatc cacatgcctg cttttacgcc gtttacccat
    19261 ggaggcgggc ctatcatccc cggcaaactt ttcccctatc ttttcattac cattgcctgc
    19321 ggggctatca gcggatttca tgccctggta agttccggaa ccaccccgaa gatgatcatg
    19381 agcatgaagg aggtgaaatt tattgccgcc ggggcaatgc tttcggaagg aatggtcagc
    19441 atccttgccc tgattgccgc taccagcctg ctccccctcg attatttcca gatcaatgtc
    19501 cctgttgaaa agttccaggc catcctgccc cagcttcagg ccatgggctt caccgaatcg
    19561 aacctcaatc agctatcggc cgaagtcggc gaaaaaatag ccggaaggac cggcggcgca
    19621 gtctcgctgg gcgtcggtat ggcgtacatc ttttcatcca ttcccggatt gcagcacctg
    19681 atgagctact ggtaccattt tgccattatg ttcgaggcgc tgttcatcct taccaccatc
    19741 gatgccggta cccgcatcgg gcggtttatc ctccaggaaa cactcgggaa aatttataaa
    19801 ccttttggcc ggaccaactg gctgcccggg aacctgcttg ccagcggact gatagtgctg
    19861 gcctgggggt attttatata cacaggcagc gtttcgacaa tctggcctat gttcggcacg
    19921 gcaaaccaat tgctggctac agtagcattg gcaatcggga cttcattcat tattaaccgg
    19981 ggactggcgc gctattcctg gatgaccatc atcccgatgg tttttgtagg agtgacgacc
    20041 ctctcagccg cttacatgaa catgacaaat atttacctgc cccagataca cgaccccgtc
    20101 agccgggttc agggcatcat cagctttacg cttaccgcga tcataattct ctgcgtactc
    20161 gtcgtattta ttgatgctat acccaaatgg atcgccgtca tgagaggaaa gagagcggtt
    20221 gtaacagtca aggcataacc ggtccagcct ggtgaaagcg cgggataact accgagattt
    20281 catcgtaaag ctatcggaaa acggcaacgc cgttcatgcg tttatctaaa cggtaacgat
    20341 cgtaacagga ttgtgtgatc aacaatccca agtgtctgat tgagactctt ttataggtat
    20401 ttcttatttc caggctttca ctaccagccc tgttccactt ttgtggtctc ctgttacgtc
    20461 tgcccggttc aggattaagc tgatcgggaa tgtgatcagg taatataagg gaaggataat
    20521 aaagaacagc ttcgatttcc ccagcatcag gatgggatac ttcattgaga gtttccagga
    20581 taactgtccc ggttttccat actggtactt tgcttcggta cggctgaagc cggctttctt
    20641 cagtttttcc tgaatttcac cgatgttgta cccttcgcgg acatgttccc cgatgaaaga
    20701 ttcctcgtca tggtcgtgaa catcactgcc tccctggtcc gacggggtgg agataagcac
    20761 catgcctccc ggcctgagcg aggcgtggaa gttttcaaac accttcacat cttcagtgat
    20821 gtgttccatc acatccaccg aaaggatgag gtcataagcc aggggttccg aaaacgctgt
    20881 gaggtcagca taagagaagg aagcattgtt gcggccgatg cgtttgaaaa aagcattgca
    20941 atcatcgatc tgttcctgtt ttacatcgac gcctttcacg gtgtaaccgg ccttcatccc
    21001 ggaaaggaaa taggaatact gcccgaagcc catgcctgca tcaaggatgg cggcgtttgc
    21061 cggcgccagc ttcgaccatt ggcgcaaatt cctgcggata tgccacgaac gcagcaacaa
    21121 caaatcaagc aggtgataaa acatctttct cagccgggga gtccggttaa aaacagttcc
    21181 gagcgatcgt ttgatggggt cgtattgcat aaggacggag ttacggggtt gagagcctgc
    21241 ctgtcaggca cagacctgca aggggctggc aggtttgaga gtttgagggt ttccggataa
    21301 taatctcagc gaagctgtaa accatgttct gattactatt actctttcct gcaatgaact
    21361 aactcttcag caggttttca atgttttcga tataatcgag tgaatcgtca ataaagaatt
    21421 ccagttcggg aatggaacgc acctggttgc ggatgcggtt tcccagtttc agcctgatct
    21481 cctttttgcg ggtgttgatc tcatccagca gctctttttt accggttttg ccaaaaaggc
    21541 tcaggtatga cttcgcaacg tacaggtcgg gcgaaatatt aacttttgta acggtgatca
    21601 tgttgcccgg gaaaaggtca cggcacataa cctggaatat ttcacccagg tctttctgta
    21661 tcagccgtgc aatcttttgt tgacgtgttg tttccataat acaaaggtac aataattatg
    21721 aaagggagaa gtcctgcccg gctactgcac tttccgatgg ctgaatactt taaccggata
    21781 aactttatta ttttattttt gcacagataa aagtaacaag ccattacggc ccatcattcc
    21841 tgacagatac ttttttgtga aaaaactttc gactttactt cgctacgcat acccatacaa
    21901 gtggcatgcc ttgctcaaca tagtattcaa cattctcggg gccatatttt cactggtttc
    21961 actggcctcg ctcgttccgg ttcttaacat cctgttcaag cagtcggagc cggtctcggg
    22021 cctgatgcca tgggcgcttg acaccaaagt cgttttcaat aatctcaatt tttacatcag
    22081 ccaggaaatt acccggagcg gcgaaattaa tacgttgctc gtcatttgca ttggcattgt
    22141 catcctgttt ttactgaaga atttctttcg ctatatggcg ctgttttatg ttgccgtttt
    22201 acgcaacggg gttgtcaaag atctccgcaa ctcgctatac cataagatac tgatccttcc
    22261 cctggcgtat tattccgatc agcgtaaggg cgacattatt gccaggatca ccagcgatgt
    22321 tcaggaggtg gaatggtcga tcctgtcgtc gctcgaaatg cttttccgcg acccgatcac
    22381 cattattatt tacctgattg ccatgttcaa gatcagcccg cagctcacac tgtttgtgct
    22441 ggctgtattg ccggttgctg cctatttgat cgggctgata ggccgcagcc tcaggaggac
    22501 ttcccaaaaa gggcagaaaa agatgggaga actgctctcc ctgatcgagg agaccatttc
    22561 agggctcagg atcatcaaag gtttcaatgc cattgattat tcctaccgca atttcgacga
    22621 aaataacagg gggttcatgc ggctgatggt aagattgttc cgcaaacgcg acctggcgtc
    22681 gccggtcagc gaattcctgg gtatcgccgt gacggcgctt attttatggt atggcggcag
    22741 gctgatattg tcgtcggaag catccctcac cgcgcccaaa ttcatcctgt acattggcat
    22801 tttctcgcag atgatcgtcc cggccaaggc catcactact gccttttata acgttgaaaa
    22861 agggtcggca tccatccagc ggatcaacca ggtgctcgaa gccggcgaag tgattgaaga
    22921 aaagccggac gcccttgcgg ttgatgagtt ccgggataaa atagaatacc gggatgtttc
    22981 tttccgatat gataaagatt tcgtgcttaa caatataaaa ctcaccattc ccaagggaaa
    23041 aatggttgca ctcgtcggcc cttcgggcgg cggaaaatca actatggttg atctgctgcc
    23101 ccgcttttac gatgtaacgg aaggcggtat tttcatcgac ggaaaggatc tccgcgactt
    23161 gcggatcgac aatcttcgcg ggttgatggg aatcgtgaac caggaaacca tccttttcaa
    23221 cggaaccatt gccgacaata tcgcctttgg catgaaggat gcccgtatgg aggatatcgt
    23281 tcatgctgcc aaggtggcca acgcccatga attcatcgaa agacttgaga atggatacaa
    23341 taccaatatc ggcgaccgcg gaataaaact ttcgggcgga caacgccagc gtatcagcat
    23401 tgcccgcgcg gttttaaaaa atcctccgat catgatcctc gatgaagcaa catcggccct
    23461 tgataccgaa agcgagcgcc ttgtgcagga agctttgctc aacctgatga agaaccgcac
    23521 ttccatcgtt atcgcccaca ggctttccac catccagcat gccgatgaaa ttgtcgttat
    23581 caacgaagga aagatcacag aacagggaac gcataatgaa ctcctggcag ccaatggcat
    23641 ttacagccgt ctgtgcgaaa tgcagtcttt cagttaatac cggtgttact tctgtaagtg
    23701 tcctgtcaca tcggaccaaa tgaaccatta tctgattgcg tataccggaa tttttctatc
    23761 tttgcaccgg acattccgga aaaatcagtt ttaaactatt tgatgtttgt ccatatctca
    23821 caaccacaaa catcctatcc agaagagcat ctgatggaaa agagaattgg aactatcacg
    23881 atcttaatca aagggactgt ttctgtacct cccgtaaatt ccctgttatc ggaatacggc
    23941 agtatcatcc ttgcccgcca gggccttccc atgcatcatc gtaatattca tgtgatatcc
    24001 ctgatcatcg aaggatcggt tgaggttatc aatgctctta ccggaaaaat aggacgtttg
    24061 aaagatgttg aagtcaaatc ggtcctaaca aaatacaagg aggagcaaaa taatgaacaa
    24121 gaatgaaagg aataaaattt tcatcgacag ggataaactt tattccctga tagaaggaaa
    24181 agtccccgga gaatcagaag tcaatgatat tctgaacaaa gccctgaagt taaaaggctt
    24241 aaatcttgaa gacgtagcgg cgcttttgcg catagaagat ccgcgcctga tccataagat
    24301 catggagacc gccaaaactg ttaaagaaga aatttacggt aaaaggctgg ttctttttgc
    24361 tcccgtgtat accggcaatg catgtatcaa taactgcacc tactgctctt tcaggaggga
    24421 caacaaactg ataaaacgca aagtcctcag catggacgag attgaagaag acacacgggc
    24481 ccttcttcgt gaaggccata agaggatcct gcttatttgc ggcgaaagca agcgtaatgg
    24541 tacggattac atggtagagg ccatccgcag ggtatacagc atcagggaac acgacgggag
    24601 ggactatatc cgccgaatca acgtggaatt ggcccccatg gaagtggaag attttgccag
    24661 gctgaaagcc gagaaaatcg gaacctatgt ttgtttccag gagacttatg atccggtgct
    24721 gtaccccaaa tttcatcctg ccgacacaca aaaaggcaat tatgaatatc gcttaacagt
    24781 catggaccgt gctatggaag gcggaatcaa cgatataggc atcggcgccc tgcttggcct
    24841 tattgattac cgtttcgaag tgcttgccat gatagagcat gccaaccacc ttgaaagggc
    24901 tttcgggtgc ggcccccata ccgtcagcgt tccgcgcatc gaatatgcca tcggcgcacc
    24961 tcactcggag aatatcccct cgaaagtttc cgacgaagat ttcaagaaaa ttgtatccat
    25021 aatcaggatt gcgcttccat acaccgggat tatactcagt accagggaaa acgataccat
    25081 gcgcaatgaa ctgataaaat acggcgtcag ccagttgtca gccggatcaa gggtgaaccc
    25141 cgggtcctat gcccatgagg atcagcatac gggaagccag ttcaccctgg gcgaccatcg
    25201 ctccctcgat gaagtgatct cgtccctggc atccgaaagc tacattcctt cgttttgcac
    25261 cggctgttac aggaaaggaa gggttggaca ggatttcatg gacctcgcca agcccggact
    25321 gatcaaacag tactgtttgc ccaacgccat gttcaccttt acagaatacc ttgaagattt
    25381 tgcatctccg gaaacgaagg aaaaagggct ccgcctgatc gaaaaactgg tcggcgaagt
    25441 agaaaagaaa ccgttacagg agaaaatatc cgggaatatt tcgagcatca ggaacggaca
    25501 aagggatatt tacttctaat atatcccgga ttttcgtttg ggtttcggca gagccgcaaa
    25561 agtgcatatc tacggcattc ttaaatctca gacgattatc atcaactgcc tgtttcttca
    25621 tcatccgggg ccgcatcatc cgcctgttgt ctcgcgtatt gaagcacctc cggatttaca
    25681 tacgaaagat cgatccctgc ctgggcaaac atctcctccg actccgaccc ggagtggtac
    25741 ttgaactcgc aaacaacccg cttaatgccg caattgatga tgagcatggc gcaggtgcgg
    25801 cagggcgtca tgcggcaata cagggtgctg ccttcgagcg ggatgcccag tctggcagcc
    25861 tggcagatgg cgttctgctc ggcatggacg gttcgcatgc agtggttggt tacatggccg
    25921 tcggcatgcg tcacctgttt gaagagatgt cctacatcgt cgcagtgtgg caaccccacg
    25981 ggcgaaccca cgtatccggt taccagtatt tgcttgttgc gggcaatgac gcagccgctg
    26041 cgcccgcgat cgcaggtagc gcgcttggcc acggtgcgcg atatttccat gaagtactcg
    26101 tcccaggtgg gacggatata agaagtgcct ttcttttcgt ccataatccg gcttattgat
    26161 ccccgaacaa cgccaccttc atttctcccc cctcttttac ataaccccgg aacattccgg
    26221 cagtgttgaa cggcatcgca atattaccgt ttttatccac tgctatcaca cctccggttc
    26281 cgccttgtgc ttttaatttt ttcatgacca cttcgccggc ggcatcctcc agcggaaacc
    26341 ccttatattt catcagggct gacatgtcgt aagccaccac attcgtggag aaa
//
LOCUS       DAOPYV010000042        24997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_1985_length_24997_cov_13.213295, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000042 DAOPYV010000000
VERSION     DAOPYV010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24997
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1985_length_24997_cov_13.213295"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKI35_07035"
     CDS             complement(<1..361)
                     /locus_tag="PKI35_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX44175.1"
                     /translation="MTFSEFGFDPRVLEGIESLGYETATPIQEQAIPPIMAGKDMIGS
                     AQTGTGKTAAFLLPVIHRIITTKHDESIKVLIIVPTRELAIQIDQQMQGMSYFTPVSS
                     IPVYGGTDGATYSNEQKA"
     gene            622..2100
                     /locus_tag="PKI35_07040"
     CDS             622..2100
                     /locus_tag="PKI35_07040"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HNX44176.1"
                     /translation="MKNIMLFILAAAMLTGCNSGKKNKETTGTDKVAAIEVKNTGNVN
                     YDQYFTGGTMRLDYFHTGNAKEEHFAVDQALMDGEWPGSRNQLIDKLELGLYYFEVAD
                     KETNTLLYSRGFASIFGEWQSTPEADSVYGTFHESLRFPWPLKPVTIILKKRDAENKF
                     RVIWTTVVDPASRQVMKAVKPHTEKVDVIMQNGPAQEKIDLVILGDGYAKSEMEKFRR
                     DAKRLTDALMTTEPFAARKNDFNVRAVETPAEASGVCKPHHNVYKRTPLSVHYSSFDS
                     ERYALTYDNRTVRDVASAVPYDFMVILINEQTYGGGGIYNLYTTVSADNKFADYIMVH
                     ELGHHIAALADEYYTSSVAYEVPDIKVEPWETNITAMFDKSKLKWKELVEPGTPLPTP
                     WNKEAFDKYGYRVQVTRDSIRAALLPESVMEELFVKQMAAEDSYFAGEKYKGKVGAFE
                     GAGYTPKGLYRSQLDCIMFTRHQLFCKVCCRSLENVFDQYTK"
     gene            complement(2103..2285)
                     /locus_tag="PKI35_07045"
     CDS             complement(2103..2285)
                     /locus_tag="PKI35_07045"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HNX44177.1"
                     /translation="MTKLFGGFTEEFYTAYNDAFPLEKGWQQRIDILNLYPLLIHVNL
                     FGGGYTSQVKSVLSRF"
     gene            complement(2309..2977)
                     /locus_tag="PKI35_07050"
     CDS             complement(2309..2977)
                     /locus_tag="PKI35_07050"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HNX44178.1"
                     /translation="MIPPAIQTEIGSFFSREYGTGIRVLSSSGIGGGCINDAAWFETS
                     AGKFFIKYNLASRYPDMFEKEALGLKLISGMGNIRVPEVVFSGTAGEYTYLVLEYIEQ
                     GTRRRDFWDDFGRSLAALHRNSAKAFGLDLDNYMGSLRQSNRFHDEWHDFFISERLEP
                     LVKLARDAHEISSATVSSFENLYLRLPDIIPREQPALLHGDLWGGNFMVAEEGKVCLI
                     DPAI"
     gene            complement(3229..3651)
                     /locus_tag="PKI35_07055"
     CDS             complement(3229..3651)
                     /locus_tag="PKI35_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44179.1"
                     /translation="MIPKELQSDEAIRRLLSYTAPESPSDDFTARLMEKILDEPVREI
                     SWINRNQWLLFFTVAAILILLFIFPIWSLFGYEFTPGQFFMYYAGDFVSKTALWLGEQ
                     LSRFGSLGKVWYLVPVSVAIVLLAAFDQAIHKPAQANA"
     gene            complement(3678..4256)
                     /locus_tag="PKI35_07060"
     CDS             complement(3678..4256)
                     /locus_tag="PKI35_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNX44180.1"
                     /translation="MKDQDDIYHVQRILAGNTASFAYLVNRHKDMAFTIAFKIVRNRE
                     DAEEIAQDAFVKAYQSLRSFKGESKFSTWLYRIVYNAAISHTRRHRFEFTVLDEAMTG
                     NITEDEIFGSLDVMDPEERSQLVTAAVDKLLPEESALVSLFYMEDNSIEDISQITGLS
                     VSNVKVRLHRIRRKLYDSLQPKMAGTEMAQMM"
     gene            complement(4449..7586)
                     /locus_tag="PKI35_07065"
     CDS             complement(4449..7586)
                     /locus_tag="PKI35_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNX44181.1"
                     /translation="MSRGLIIFLMLMATGVAAAQKTSQNPSRIIWSEDFSGGKIPAGW
                     IVKAENDSNVMWECTNQPFPGSFGRDQQAPPVASKSGGFHMQFAPGVRVDKFYKRWEK
                     AKIWPDAYFQTSPVDCSGRSSVVLKFQQNFMWNDWGKVRDDGGLFVGVSADGQHWKEY
                     EVRNGIGSEGNCPNPMNVELNITGTAAFQKTVYLRFSWRGIYAWYWMVDNLELSEALD
                     NDLSASALVSHPQSGNSFTAADVFRFHVVNLGSGPVTQPFDCYLRIDNRPPFRITVPF
                     SEKQPLKIIDTVEAVFPPADLTDLGIHKIRFYTSLPGDSRRENDTLDMELYSQAYEIG
                     PVTGFERSGNSFVFSCNRAKVRVDFARDDIFRIRMSYNGEFSDPVGSDIVINPPSGDV
                     KTEFTDAGNYYLMKTSGVALRAYLSPLRFAMYKPDNQTLVWEERKDLTYGKETVQYLS
                     CGDDEYFYGGGMQNGRFSHRDKTILLRIDWNWEDGGAPNPAPFYMSTRGYGALRNTYA
                     PGEYSFSDTVTTKHAEARFDCYYFTGESLKDILNAYTDITGKPFLPPRWALGMGDANC
                     YNRGENKGKNTTGYAGTTPDVIPLVADKYIENDMPRGWILPNDGYGCGYTKLDSVVVE
                     LHKRGFHTGLWTENGVEKIAREVGLYGSRLCKLDVAWVGEGYKFAIDGCKAAYEGIEN
                     NSTARGFVWSVCGWAGTHRNSVVWTGDQSGTWDYIRWHIPTVIGSGLSSQNCATGDID
                     GIFGGSAKTFTRDLQWKCFTPVLMSMSGWAPKDKQPYVYGEPYTSINRKYLKLKMRLT
                     PYMYTLCHEASVKGVPAVRALVLEYPDDPVARGTETQYEYLLGRNFLVAPVYSDTNIR
                     DHIYLPAGKWFDYWDGTVYDGNRYLDGYPAPLAKLPLFVRAGSIIPMYPQMNYDNERP
                     ADTLTLDIYPGPGATFELYEDEGSDRGYRKGKFSLTHLEAFTDAANRVTGVKVSPARG
                     EFPGKLANRAYILKIHTTAVPKQVKARGDKVKQGTTTGLKQGWYYSPDEKQGTLTIFT
                     GHIPAEETTAIELKY"
     gene            7847..8200
                     /locus_tag="PKI35_07070"
     CDS             7847..8200
                     /locus_tag="PKI35_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44182.1"
                     /translation="MQAEIFVPVSMFAMILGIVYLSYRKKERMALIQSGKDASIFGQE
                     RKCYSTLKWGLLLFGIGLGLLIAEALARYDVLSPEVAYFSMAFIFGGLALIIDFLLEK
                     IRKDKEEEKNEPPKA"
     gene            complement(8891..9466)
                     /gene="thpR"
                     /locus_tag="PKI35_07075"
     CDS             complement(8891..9466)
                     /gene="thpR"
                     /locus_tag="PKI35_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNX44183.1"
                     /translation="MKRLFAAIKINPSAGFTATVNSLVSELRYERIKWVETGNMHLTL
                     KFFGETEEKRIPAINAALKAAASKTPAFTLRIGRTGIFGSRYDPRVIWFGIDPEPTLQ
                     QLAADVFAELEKAGWEPDRQNFVPHLTIGRIKELKDRLLFQDVIQKYRETFIQEQPVY
                     EVILYESILQREGPQYIALQKFPLLTPPARS"
     gene            9610..9683
                     /locus_tag="PKI35_07080"
     tRNA            9610..9683
                     /locus_tag="PKI35_07080"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9644..9646,aa:Ala,seq:ggc)
     gene            10152..11258
                     /locus_tag="PKI35_07085"
     CDS             10152..11258
                     /locus_tag="PKI35_07085"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNX44184.1"
                     /translation="MAYDPNIPYNDLPGLPPALDTDTQTVLLTHALKAARSLAELKGL
                     CYSMTGEALMNLLVNTIVLQESRDSSAIENIVTTQDELYKAVAGDQAVNQATREVLHY
                     REALYVALQYMRDHNNLITSNLMVRAVQAIKQNNAGIRNQPGTLLLNAITGETIHTPP
                     CCEEVIRQKLAALETFINDPLFSDFDPIIKLALIHYQFEAIHPFADGNGRTGRILNAL
                     YLVQQKLLPQPVLCLSAYIVDNKELYYRLLRDVTTTGNWKDWILFMTTAVDSTAKLTM
                     KKIRSIQDLKKEMEPAVLKVLEKFGNRMQLLDLMFTMPYLKIEHLVSKGIAHRETASN
                     YLKLLVAEGLLASSQVGRNLYFTNHRLMDILTQH"
     assembly_gap    11260..11359
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11942..12826)
                     /locus_tag="PKI35_07090"
     CDS             complement(11942..12826)
                     /locus_tag="PKI35_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44185.1"
                     /translation="MDEHLKSIDKQIEFNQGKNVFLDDNETKLRFVDETIKAISNIGE
                     LNPGDENILTDYATDKAIEEFCRINQYYTFNPQAKNDLREIYSKLFSVIKAGKSPLEA
                     IEKDHYENLKRWLQKTNPFAEKIYAGADRDINPVACSEYGAELQLDILHLDIRTLTNP
                     VLDIGCGKHGTLVGYFRQLGFDATGIDRFTFPEATLINSDWLEFAYGTEKWGTITSNL
                     GFSNHFKHHNLRDDGNYMEYARKYMDILKSLKTGGKFHYAPDLPFIERYLDHQQFEIE
                     QFEIGGFDFKTTVIKRLK"
     gene            complement(13010..14080)
                     /locus_tag="PKI35_07095"
     CDS             complement(13010..14080)
                     /locus_tag="PKI35_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNX44186.1"
                     /translation="MNDFKTRNYIALIVGFVCILVILFGHRLLPVGFFYAEMVSYLVC
                     LTLIILVLRFVLGLSLRDHFFSYADTGEGSLLKKFPLLSGLLLSLLLNLTFLLIGKSG
                     FNNLLGGRDMNLFQNIFLVVAGAPVIEEVLFRGYIQDLIKAGFFSRQNRNVTTWFPIV
                     ITSLIFAALHLFYLGTAAPLQLVFIVMLALSIGLLSGFLRSKYHHIKYSVKLHIGANL
                     GGVLVIPVALLAGIIGKPEFQEMTKTKEACQFDMNNNAAFSEDLSQFFSSELKLNDAA
                     EKTAINCWIPCLIHCDKNGNILSVSLDTAQAKRSGYDCQTPGIEEDALKTLKKLPKFV
                     PAKGMTKDTTIQHTFYVYNPGK"
     gene            complement(14266..16926)
                     /locus_tag="PKI35_07100"
     CDS             complement(14266..16926)
                     /locus_tag="PKI35_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNX44187.1"
                     /translation="MLDTPTKKRIDDCRDILVGKLPDPKAQIEQITIALIYKFMDDMD
                     KEAIELGGKAKFFSNYTIPDPKEPGKTILVEFEKYSWNNLFDTKVTANEMLMLYSDAI
                     AAMDKNPNIPQLFRDIFKNAFLPYRDPVTLKLFLNTIGEFEYTHSEKLGDAFEYLLSV
                     MGSQGDAGQFRTPRHIIDFLVAVVDPQKTDTILDPACGTAGFLISAFKHIMDGKDAIT
                     PDERRRIIGNFTGYDISPDMVRLSLVNLYLHGFSDPHIHEYDTLTSEERWNDTFDVIL
                     ANPPFMSPKGGIRPHKKFSISSSRSEVLFVDYIAEHLNPKGRAGIIVPEGVIFQSGTA
                     YKDLRKMLVENYLYAVVSLPAGVFNPYSGVKTSILLLDKELAKTRDEILFVKIDNDGY
                     GLGAQRNPIKGGQLKDAITIINDFRCFCRDANNRVPTKTTTPPAKPQNPIAHTVPKPE
                     IGKNGDYNLSGERYKTQIISNTAYPLKPIEELFESIRNGRNVEQIDEVGNYRVTRIQT
                     IADKTVDLEKTKWTNDEVPEDYFMEEGDILLSHINSFEHLAKSAIFKGISEKVVHGTN
                     LIRFKPNKNKIVPEYAAAIFKSEFFIDKAKTFAQKAVNQASLRVADIKSIQIPLPPLS
                     VQEEIVAEIEGYQKIIDGAKMVVENYKPKIDIDSYRDDPEWEMVELGEVCEVNPKKSE
                     LANVSRKIEVSFVPMADINENEISFHPKQVKPLGEVIKGYTYFKEDDVLLAKVTPCFE
                     NGKAGIAKGLVNGIGFGSSEYFVLRPNDKILPTWIYLNVITNSFREKGKLNMTGTGGL
                     QRVPPDFIKSTLIPLPAIDIQRQIVSRIEKEQELVNANKQLIELFEQKIKDRIAKVWG
                     EGPHQGEALNAPNEQDFGLAAEPLADYKTK"
     gene            complement(16978..17760)
                     /locus_tag="PKI35_07105"
     CDS             complement(16978..17760)
                     /locus_tag="PKI35_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44188.1"
                     /translation="MSDKFQNKYRIPSARLQNWDYGSNAAYFVTICTQNRENYFGKIV
                     ETPIVKMELSELGKIANQIWNEIPIHFPFVELGEFVVMPNHVHGIIVIDKPVSGDGRD
                     DDGRGGRDAINRVSTTTETATGTTTETATDTTTGTETGFKTGFETETKSEMETDPDTD
                     PDTDTISESEMESEMESETHIGGFAGNKNPMLNNNLSRIVRWYKGRVSFELRKIHAGF
                     AWQSRFYDHIIRDDMSFQRISKYIVNNPSKWADDKLHDGTIL"
     gene            complement(17762..20293)
                     /locus_tag="PKI35_07110"
     CDS             complement(17762..20293)
                     /locus_tag="PKI35_07110"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNX44189.1"
                     /translation="MLQNEAHARIKINQLLDEAGWRFFDNHEEKANILLENHVKITQK
                     EIDAWGNDYEKVKSGSLDFLLVDTDNKPICVLEAKRESLHPLVAKEQARKYANIVGTQ
                     FIILSNGIVHYLWDLKKGNPKPIYKFPSPKEIGAIKDWNPDRNSLATEPVGGDYIVTV
                     QMPDYAQRPGWNGSISTSKDFIRTNGLRFLRPYQLSAILHLQKAVREGKDRFLFEMAT
                     GTGKTLTAAAVIRLFLRTKNARKVLFLVDRLELEDQAWKAFTNYLKPDYTTFIFKEHK
                     SDWRKADIVVTTIQSLMFDNKYRSQFSPTDFDLIISDESHRSISGNARAVFEYFYGYK
                     LGLTATPKDYLKGVDPDKVKENDPREIERRMLRDTYATFGCEGGDPTFRYSLLDGVKD
                     GFLVNPRVLDARTEITTQLLSDEGYAVAITTEEGEETETFISRDFERKFFSDKTNRVF
                     CHTFLENALRDPITNEIGKTIIFAVSQNHARKLTEILNEFAEQLFPDKYNSDFAVQVT
                     SQVGEAQQMTINFTNNNLNGKTTWLEGYKSGKTRICVTVGMMTTGYDCPDLLNLCMMR
                     PVFSPSDFVQIKGRGTRKTTFEFRHKNELGEIEHVKHEKEFFKLFDFFANCEYFEEKF
                     DYDEKLKLPKPRNGEGEGGGTGVDIDKYTSFRPDPLAVLNEQQIGYEGMKIDRMLFKK
                     FEDRIVMDDIIKKNVELGNWEQVVSHIQNEIFDKPEEYFSLEKLRKAAKIDRKVSIRE
                     MVEKVFGIIPKFKSKDELLEEEFDKFISIYPPEEDVNLRALKYFFKAYIVDGEIRKII
                     EAKDFHALQTNPTLTISQFKEVAVKYREVIPLYIKDYINLDRFAA"
     gene            complement(20875..21564)
                     /locus_tag="PKI35_07115"
     CDS             complement(20875..21564)
                     /locus_tag="PKI35_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX44190.1"
                     /translation="METLASKPKVLIIDDEVQIRRLLRITLEANDYHVIESENGKNGV
                     LLSAMEHPDVILLDLGLPDMDGLQLLERLREWSAVPVIILTVRDEEKVMIKALDSGAD
                     DYVTKPFNTGELLARIRVAIRHSLRIDESPVFTRGRLSVDLTNRIVKVNNEEVRLTAL
                     EYNLLSLMVKNAGKVLTHAYIIRELWGNAYADNAQTLRVHVAQLRRKIEANPSIPEML
                     ITEPGVGYRLV"
     gene            complement(21565..23499)
                     /locus_tag="PKI35_07120"
     CDS             complement(21565..23499)
                     /locus_tag="PKI35_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX44191.1"
                     /translation="MKTSERQSSGTGTPGRRLMILLGSEPSAGSFLQEIRQQYADSDG
                     EWFVVCAEPGPGAGKAVRKQFSNNLTQALLSGARVIHLLDYDVVDGVTNLIDRYSVNR
                     LLIGNFKPVKITLGENTGLLIRRLQKKNPDVEITQIKMKKEAQKINAANIKIFTRPKE
                     YLYAAMAVVITSAACFPVKEYIGYQTVGLILLILTAILSLFLGRGAVIFTAILNFGAW
                     NFFFIPPILTFHIASFHDMIMLFANLAVAITGGSLINRLRKNQADLELSRKNITLLYS
                     LLESLNNANSIKGVIGKVRAELDRHFDADAIVYLREKQGPSLEKRAFGNDGFFSETEF
                     EYACLVFQMKERAVVRRLNDTLSIRYFPLSAQSGTLGVIGIATKTGTGIDEEKLIFLK
                     SFITQITSALEREISIDKAKENQVYQESQKLFQTVLNSVSHELRTPVSIISSAVSNLM
                     DDKTAADPVNRNSICTELEASARRLNQLIENILDMSKIDSGYLSLNLQPCDICDLVGV
                     VVNHMKDELSDHAVRVDIPDLLPAVKIDMNWMKQALMNILRNSAIYTPRGSEISISAS
                     ADGETAVLKIEDAGPGVPTASLDHLFDKFYRVPGSRSGGTGLGLTIAKALVEAHNGTI
                     KAVNRPQGGLSVAIQLPIAE"
     gene            23908..>24997
                     /locus_tag="PKI35_07125"
     CDS             23908..>24997
                     /locus_tag="PKI35_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNX44192.1"
                     /translation="MKNDFKLPSDQQLREFLIGKARRLSDSGTFHKISLIAFLAWIGL
                     GTDGISSSAYGPEEAFRYLGQHHDLALFVALGTIFTIVIISTSYKQIIRLFPHGGGGY
                     LVASKLLSPTVGMISGSALLIDYLLTITLSISSGADAVFSFLPPRYHSLKLIVAVLAL
                     VLLILINMRGVKESVLSLTPIFVLFMATHVFIILYIFFSNSAAMAGVVAESAEGFGSS
                     VSQLGFVATMFIILKAYSMGAGTFTGIEAVSNGLPILREPRPVTARKTMNLMALSLSF
                     LVFGLLMGYSLYQVRADPMKTLNAVLLESTTAGWNRLAGYSFVTVTLFSEAALLFVAA
                     QTGFLDGPRIMANMAADQWLPKRFASLSD"
BASE COUNT         6142 a         5829 c         5890 g         7036 t
ORIGIN      
        1 gggctttctg ttcgttgctg taagtggcgc cgtcggtgcc gccataaacg gggatggagc
       61 tcaccggcgt gaaatacgac atgccctgca tctgctgatc gatctggatg gccagttccc
      121 gtgtcgggac aatgatcagc actttgatgc tttcgtcgtg tttggtggtg atgatccggt
      181 ggatcaccgg cagcaggaaa gcggccgttt tgccggttcc tgtctgggcc gaaccaatca
      241 tgtccttccc ggccatgata ggcgggatag cttgttcctg gataggcgtg gcggtttcgt
      301 aaccgaggga ttcgatgcct tcgagcacgc ggggatcgaa gccaaattcg ctaaaagtca
      361 atatactggt ttttttgcaa aggtacggtt cccctggtta tcaggctccg gagaatagag
      421 gggaacgtaa aaaaaaatca ggaggggagg ggagaacagg gagggatgat tgccgttgcg
      481 acctgtcagc gtctgccggg aagcagacct tatatcatcg gcatcaccgc cgtgcttcag
      541 gaatggcttg ttgttgctaa aagtcaagcc tttgactgca ccctatcaca aactgtttta
      601 tcttcacaaa aaaattttac catgaaaaat atcatgttat ttatcctggc ggccgccatg
      661 ctgacaggct gcaattccgg gaagaaaaac aaagaaacaa ccggcacgga caaggttgcc
      721 gccattgaag tgaagaatac cggcaacgtg aattatgacc agtattttac cggcgggacc
      781 atgcgcctcg actatttcca caccggcaac gccaaagaag agcattttgc tgtcgatcag
      841 gccctgatgg acggcgaatg gccggggagc agaaaccagt tgattgataa actcgaactg
      901 gggctgtact atttcgaggt tgcagataaa gaaaccaaca ccctgcttta ttcccgcggc
      961 tttgcaagta ttttcggcga atggcagtca acgccggaag ccgacagcgt atatggcact
     1021 tttcacgagt cattgcgttt tccctggcca ctaaaaccgg ttacgataat tctgaaaaaa
     1081 agggatgccg aaaataaatt ccgggtaata tggactaccg ttgttgatcc tgcttcgcgg
     1141 caggtgatga aagccgtaaa accgcatacc gaaaaggtgg atgtaatcat gcaaaacggt
     1201 ccggctcagg aaaaaatcga ccttgtgatc ctgggcgacg gttatgccaa atccgaaatg
     1261 gaaaaattcc gcagggacgc caaaaggctt accgatgcgc tgatgaccac cgagcctttt
     1321 gctgccagga aaaacgattt taacgttcgg gcggttgaaa cgccggcaga agcctccgga
     1381 gtatgcaaac cgcatcacaa cgtttacaag cgcacgccgc tttcggtgca ttacagcagc
     1441 ttcgacagtg aacggtatgc cctcacttat gacaatcgta ccgtccgcga tgtcgcttcg
     1501 gccgtgcctt atgatttcat ggtaatcctt atcaacgagc agacctatgg cggtggcggg
     1561 atatacaacc tttatactac cgtatcggca gataataaat tcgccgacta tatcatggtt
     1621 catgaattag gccatcatat tgccgcgctg gccgacgagt attacacatc gtcggtggct
     1681 tatgaagtgc cggatatcaa ggtagaaccc tgggaaacca atatcaccgc catgttcgac
     1741 aagagcaaat taaaatggaa agagcttgtg gagccgggaa cacccttgcc cacaccctgg
     1801 aacaaggaag cttttgataa atacggctat cgcgtacagg tgacgcgcga cagcatcagg
     1861 gcggccttgc tgcccgaaag tgtcatggaa gagctgttcg tgaaacaaat ggctgccgag
     1921 gattcttact ttgccggtga aaaatataaa ggcaaagtcg gagctttcga aggcgccgga
     1981 tacacgccca aaggtctgta ccgttcgcaa ctcgactgca tcatgttcac gcgccaccag
     2041 ctgttctgta aagtttgctg ccggagcctc gaaaatgtct ttgaccagta tacgaagtaa
     2101 tactaaaacc ggctgagaac agacttaacc tgagaggtat aacctccacc aaacaggttg
     2161 acatgaatga gcaacggata gaggttcagg atatcaatgc gttgctgcca gcctttttcc
     2221 agcggaaaag catcgttgta agccgtataa aactcttcgg taaagccgcc gaagagtttt
     2281 gtcatggcta gatcggattc acggtgtcct atattgcagg atcaatgagg cagaccttgc
     2341 cttcctctgc aaccatgaag ttgcctcccc ataaatcgcc gtgcaacaag gcgggctgct
     2401 cccgtggaat gatgtccggc aaccgaaggt ataagttttc gaatgagctg accgttgcag
     2461 atgagatttc gtgggcgtcg cgggcaagct ttaccagtgg ttcgagcctt tcggaaatga
     2521 agaaatcatg ccattcgtca tgaaaacggt tactctgcct gagcgatccc atgtaattat
     2581 cgaggtcgag gccgaaggct ttagccgaat tgcggtgcaa cgccgcaagc gaacgtccga
     2641 aatcgtccca gaaatccctg cggcgggttc cctgctcaat atattccagt accaggtaag
     2701 tatattcgcc ggcagtaccg ctgaaaacta cttccgggac tcggatgttt cccatgccgg
     2761 aaatcagctt caaacccagc gcttcttttt cgaacatatc gggataccgg gaagcgaggt
     2821 tgtatttaat aaagaatttc cccgcggaag tctcaaacca ggccgcgtcg ttgatgcatc
     2881 ccccgccgat accggacgaa gaaagtacgc ggatgccggt accgtattca cggctgaaaa
     2941 aggatccgat ttccgtttga atagccggag gaatcatagg ctgcaacagt aacaattttt
     3001 aaacctcaga tttattctga tgttgcaaag attacgaaac aaatcttttc atttcattcc
     3061 tgatcatcgg aacatgagaa ataatcagga agccaggcag gatttcttac ctggggaatg
     3121 tagttgaata aagccatgca atgtaatcgt gcgacagcaa aggaaaaggg ataaatggca
     3181 ataaaatgcc ggctcagttt gttaaacaga aaaataaagt cgcgatgatc aggcatttgc
     3241 ctgagccggt ttatgaattg cctggtcaaa agcggcaagt agcacgatgg ccaccgaaac
     3301 aggaacaaga taccaaacct ttccgaggct gccgaaacgg ctgagttgct ctccgagcca
     3361 cagggcggtc ttgctgacga aatctccggc atagtacata aagaactgtc ccggtgtgaa
     3421 ctcatatcca aaaagggacc agatgggaaa tataaacaga aggatcaaaa tggcggctac
     3481 agtgaaaaat aacagccact ggttgcggtt aatccagctt atttcgcgga caggctcgtc
     3541 cagtattttt tccatgaggc gtgccgtaaa atcatcggat ggcgattcgg gagctgtgta
     3601 actcagcagc ctgcgtatcg cttcatcgga ttgtagttct ttcgggatca tggttttgtg
     3661 tttttcgggt tacaaagtta catcatttgc gccatctccg ttccggccat tttcggttgc
     3721 aacgaatcat acagtttcct gcgtatccgg tgcagcctta cttttacgtt cgagacgctg
     3781 aggcctgtaa tctggctgat atcctcaatg gaattatctt ccatataaaa cagggaaacc
     3841 aacgcactct cttcggggag cagcttgtca acggccgcag ttaccagttg cgaccgttct
     3901 tcaggatcca tcacatcgag gctcccgaaa atctcatctt cggtaatgtt gcccgtcata
     3961 gcttcgtcga ggactgtaaa ttcaaacctg tgacgccggg tatgtgaaat ggcagcgttg
     4021 taaacaattc ggtacagcca ggtcgaaaat ttcgattcgc ctttaaaaga acgtaacgac
     4081 tggtaagctt tcacaaaagc atcctgggcg atctcctcag catcttcacg attgcgcact
     4141 atcttaaagg caatggtgaa tgccatatcc ttgtgacggt ttaccaggta tgcaaacgat
     4201 gccgtgttac ccgcaagtat ccgctgtaca tggtagatgt cgtcctggtc tttcatgtga
     4261 tgatatgacg cccctgttga caggaggtta catgtaaaag tacaaaattt cgctccggaa
     4321 ttggaaatca gcagtttgaa aatggctcaa caggctctga tgtctttcct gttttgaaat
     4381 acagtccccg caaccggtca gctgtttgaa aaattcagga cggcttatcc ggttgatgaa
     4441 tccggaaatc aatatttcag ttcgatggcc gttgtttcct cggcaggaat gtgtcccgtg
     4501 aaaatagtca gtgttccttg cttttcatcc gggctgtaat accagccttg ttttaaaccg
     4561 gtcgtagttc cctgtttgac tttatctccc cgggccttta cctgtttcgg aacggccgtg
     4621 gtatgaattt tcaggatgta agccctgttg gccagtttac ctgggaattc gcccctggcc
     4681 ggtgaaacct ttaccccggt gacgcggttg gcagcatctg taaaagcttc gagatgggtc
     4741 agcgaaaatt tgcctttcct gtatccgcga tcggagcctt catcttcata gagttcaaag
     4801 gtggcgccgg gaccgggata aatgtccagc gtcagggtat cggccgggcg ttcgttgtca
     4861 taattcatct gcgggtacat cgggatgatg gagcctgccc tgacaaaaag cggcaacttg
     4921 gcaagggggg cgggatagcc gtcaaggtaa cggttgccgt catacacggt gccgtcccaa
     4981 tagtcgaacc atttaccggc gggaaggtag atatggtcgc ggatgttggt atccgaatat
     5041 accggggcta ccagaaaatt tctaccgagg aggtattcgt attgggtttc ggttccccgg
     5101 gctacagggt cgtcaggata ttcaagtacc agcgccctta cggcaggcac ccctttcacg
     5161 gaagcttcat ggcaaagggt gtacatgtac ggcgtcagac gcatcttcag cttcaggtat
     5221 ttgcggttga tcgaggtata gggctcacca taaacatagg gctgtttatc cttaggcgcc
     5281 catcccgaca tcgacatcaa aacaggggtg aagcatttcc attgcaggtc gcgggtaaac
     5341 gttttggcgc tgcccccgaa aatgccgtcg atatcgccgg tagcgcaatt ctgggacgaa
     5401 agcccgctcc cgatcaccgt gggaatgtgc cagcgaatgt aatcccacgt tcccgactgg
     5461 tcgccggtcc agaccaccga atttcggtgt gtgccggccc atccgcaaac cgaccagaca
     5521 aaaccgcggg cagtgctgtt gttctcgata ccttcgtaag ccgctttaca accgtcgatg
     5581 gcgaatttgt atccttcgcc cacccaggcc acatccagtt tacaaagcct gctgccgtaa
     5641 agacctactt cgcgggctat tttctccacc ccgttttcgg tccacagacc ggtatgaaat
     5701 ccccgcttat gaagttccac cactacggaa tccagtttgg tataaccgca gccatagccg
     5761 tcgttgggca gtatccagcc tcgcggcatg tcgttttcga tgtatttatc ggcaacgagc
     5821 gggataacat cgggcgtggt tccggcataa ccggtggtgt ttttgccctt gttttcgccc
     5881 cggttatagc agttggcgtc acccattccc agagcccagc ggggagggag gaagggcttg
     5941 ccggtaatgt cggtgtaagc gttcaggatg tctttgagcg attcgccggt aaagtaatag
     6001 cagtcgaacc gggcttcggc atgtttcgtg gtcaccgtgt cgctgaacga gtattctccg
     6061 ggagcatacg tattacgcag ggctccgtat ccgcgggtgc tcatgtaaaa cggcgcggga
     6121 ttgggcgccc cgccgtcttc ccagttccag tcgatgcgca gcaggatggt tttgtcccgg
     6181 tgtgagaacc ggccgttttg cattcccccg ccataaaaat attcgtcatc cccgcagctt
     6241 aagtattgaa cggtctcttt gccataggtc aggtcttttc tttcttccca caccagggtt
     6301 tggttatccg gtttatacat ggcaaaacgg agaggggaga ggtaagcccg caaagccacg
     6361 ccggaggttt tcatcaggta atagttaccg gcatcggtga attctgtttt aacatcgccg
     6421 gaaggagggt ttatcacaat atcgctgccg acgggatcgc tgaactcgcc gttgtacgac
     6481 atcctgatcc tgaagatatc gtcgcgggca aagtcgaccc ggacttttgc gcggttgcag
     6541 ctaaaaacaa acgaatttcc ggatcgctcg aatccggtta ccgggcctat ttcgtaagcc
     6601 tgtgaataca attccatgtc gagcgtgtcg ttttccctgc gactgtcgcc cggcaacgag
     6661 gtgtaaaacc tgattttatg gattccgaga tcggtcagat cggcaggagg gaagacggct
     6721 tccaccgtgt cgatgatctt caggggctgc ttttcgctga aaggcacggt tatcctaaag
     6781 ggcggcctgt tgtcgatccg gaggtagcaa tcgaacggct gtgtgacggg tcccgacccc
     6841 agattcacca cgtgaaaccg gaacacatcg gccgcggtaa aggaattccc cgattgggga
     6901 tgcgaaacca gggctgatgc cgagaggtca ttatccaggg cttctgaaag ttccaggttg
     6961 tcgaccatcc agtaccaggc atagataccc ctccaggaaa agcgcagata aaccgtcttc
     7021 tgaaaagcag cagtaccggt aatgttgagc tcaacgttca tggggttggg gcagttgcct
     7081 tccgacccga tgccgttgcg cacctcatac tctttccagt gttgcccgtc ggcggaaacg
     7141 ccaacgaata atccgccgtc gtcacgcact tttccccaat cgttccacat gaaattctgc
     7201 tggaacttca gcactaccga actgcgtccg gagcaatcga cgggggaagt ctgaaaataa
     7261 gcgtccggcc agatctttgc tttttcccag cgtttgtaaa atttgtccac tctcacgccc
     7321 ggggcaaact gcatgtgaaa gccgccgctt tttgatgcga cgggcggcgc ctgctggtcg
     7381 cggccaaagg aaccggggaa gggctggttg gtacattccc acatcacgtt actgtcgttt
     7441 tcagccttaa cgatccaacc ggccggaatt tttccgcccg aaaaatcttc gctccagatg
     7501 atccgggaag ggttctgact tgtcttttgt gcggcagcaa ctccggttgc catcagcatg
     7561 agaaaaatga taaggcctct gctcatagtt gtgatatcat gggattgtac attggttgcc
     7621 tcccggcgtc gggtgggatg aaccggcaaa attagccttt cggacagata atccggtttc
     7681 ttcacggaaa atcaccggcc tgttttgcgc ccggatcatc tgaaaagggc atccgtcacg
     7741 ggcagacaag agcaggctga aaaattattt ccggagtgta acctgacggt tccctttgcg
     7801 tcaaaaatag cgtaaaccga aagtaaaacc ttaacaactc atcgatatgc aagctgaaat
     7861 atttgttccg gtctccatgt ttgccatgat ccttggcatt gtctacctat catatcgtaa
     7921 aaaagagcgt atggcgctca tacaaagcgg aaaggatgcg tccattttcg gccaggagcg
     7981 taaatgttat tctaccctca aatgggggtt gctgctgttt ggcatcggcc ttggcctgct
     8041 catcgcagaa gccctggccc ggtatgatgt tctcagtccc gaagtggcct atttctccat
     8101 ggcctttatt tttggcggac tggcgctgat catagatttc ctgctggaaa agatcaggaa
     8161 agacaaagaa gaagaaaaaa acgagccccc gaaggcatag aaggattcct cagactttga
     8221 cgccggcttt tgaaaaagaa gccggttttt ttatgccccg tttcaggggt gggtagaaag
     8281 tcaaaagtca aaggttaaaa gtttacctgc ccggggcggg tgtgggtaag acggcagggc
     8341 aaagggggga agttgatggt agaaaggtct tataggtagc ttttcgctgt tggctttagt
     8401 tttatgttct gcgttttgtg ttctgtgttt tgcgtatgga gtttcaatgt tgcaaagttt
     8461 cagtgtttca gtgtttcagt gtttcagtgt ttctgagttt cagtgtttca gtgtttcagt
     8521 gtttctgagt ttcagtgttt ctgagtttca gagttctacg ttttgagttt cgtgattagt
     8581 aattcggggt attcgtgata gcattcgtgg tatttgtggc tcgtgattcg ttctgcgttc
     8641 tatgttctgt gttttgcgtt cttccttctc cgttctgtgt tttgtgttct gtgtgttttg
     8701 tgttctatat tttgcgctct tcgttcaccg ttcaccgtac ttcgtactcc gttttccgta
     8761 ctccgttctc cgttctgcgt tctgtgtgtt ttgtgttctg tgttttgcgt tctcagttct
     8821 ccctgctctc cgctccttgc cccatgccaa ccaccggtat aaagctattt agcccgctgc
     8881 aaccggcctg tcacgaacgt gcaggcggtg tcagcagcgg gaatttttgc aaggcaatat
     8941 attggggtcc ttcgcgctgc aagatgcttt cgtataagat cacttcgtat acgggttgtt
     9001 cctggatgaa cgtttcgcgg tatttctgaa ttacatcctg aaacagcagc ctgtctttca
     9061 gttctttgat tcgcccgatg gtcaggtgcg ggacaaaatt ctggcggtcg ggttcccagc
     9121 ccgctttctc caattccgcg aagacatcgg cagccagttg ctgcagcgta ggctccggat
     9181 cgatgccgaa ccagatgaca cgcggatcgt aacggctgcc gaaaatgcct gtccggccga
     9241 tgcggagcgt aaaagccggc gttttcgatg ccgctgcctt taatgccgca ttaatggccg
     9301 ggattcgctt ttcttccgtt tcgccgaaaa atttcagggt gaggtgcatg ttccccgttt
     9361 caacccattt aatgcgttcg taacgaagtt cggaaaccag cgaatttacc gtggcggtga
     9421 atccggcgga aggattgatt ttaattgccg caaacagacg tttcatcggg tgacggaaat
     9481 aaggttgtaa cgaacagcta agttacgcag atgtttatca ccggcattat gcaccagcca
     9541 gcggcagcaa aaattataaa gttgtgctgt atatggtgaa aatattatac atttgcatcc
     9601 tctgatcatg gggcgttagc tcagttggct agagcgttag actggcagtc taaaggtcag
     9661 gggttcgact cccctacgct ccacatcaaa tgtctgtaaa tcattgattt acagacattt
     9721 ttgtatgtag tggtgacggt atgggtgact gttacagtct tgttatagtt ccattatttt
     9781 actcttataa tcatttttat tattcgaata ataagatctt agtagtaaaa tcatatccgg
     9841 tttcgaaata attatttttt aatattatac ctgtatttac acctgtaaaa tattcaatca
     9901 gctagtaatt taatgactcc cattcttaag attatggagt ttacttaatg atgttacttt
     9961 ctatcgttac tacaattaat gccttaaact ctaattttga atggccccaa aaagagggaa
    10021 gcttacaatc cgacatgagt tgtaagtaat gtcggattta ttacttttac acgacatatt
    10081 aaagaaaatg cgtcggatca tccggcataa gattcagctg atgacggaaa taaaactaat
    10141 acacgacata aatggcatac gatccaaata tcccctataa cgaccttcca ggccttcccc
    10201 ctgctttaga taccgatact caaacggtac tgctgacaca tgcattgaag gccgcccgca
    10261 gcctcgccga attgaaaggg ttgtgctata gcatgaccgg ggaagcgctt atgaatttac
    10321 tcgtcaacac cattgtactc caggagagtc gcgacagctc ggccatcgaa aacatcgtca
    10381 ccacccagga cgagttatac aaggccgttg ccggtgacca ggctgtaaac caggcgacaa
    10441 gagaggtgtt gcattaccgc gaagcattgt atgtcgcact gcagtatatg cgcgaccaca
    10501 acaatctcat tacctcgaac ctcatggtac gagccgtgca ggctatcaaa caaaacaatg
    10561 caggtataag aaatcaaccg ggaacattgc tgcttaatgc cataaccggc gaaacaattc
    10621 acacccctcc ctgttgcgaa gaggttatcc gccaaaaact ggctgctctc gaaaccttta
    10681 tcaacgatcc ccttttcagc gatttcgatc cgatcattaa gctggcattg atccactacc
    10741 agttcgaagc catccaccca tttgccgatg gcaatggccg caccggccgt atcctcaatg
    10801 ccctctacct tgtacagcag aagttgctgc ctcagccggt actctgtctc agtgcatata
    10861 tagtcgataa taaagaattg tattatcggc tgctgcgtga tgtaacaacc accggaaact
    10921 ggaaggattg gattctcttc atgacaacgg cagtggactc cacggctaaa ctcaccatga
    10981 aaaagatcag gagcattcag gatctgaaaa aagaaatgga gccggctgta ttaaaagttt
    11041 tggaaaaatt tggcaaccgt atgcagttgt tggacctcat gtttaccatg ccctacttaa
    11101 aaatcgaaca tctggtatcg aagggtatag cacaccgtga aacggcatcc aactacctca
    11161 aattgctcgt ggcggaaggc ctgcttgcat catcacaggt tggcaggaat ctctatttta
    11221 ccaatcaccg gctaatggat attctcaccc aacattagtn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnna ggaatctcta ttttaccaat caccggctaa tggatattct
    11401 cacccaacat tagttagtta atacgtccat gaaaagctct gtacacaagg acaattgact
    11461 ccaccatccg ccaggctctc atcgacaaat atggagcccc aggcaccaaa acccatcccg
    11521 gtttcacctt tgacctctca aaggataatt cgtctgcctt tgcagggcaa ggtaaaatat
    11581 cactgagaag tattaaaact ttagtgtgga gcatcacaga atcccatata cgagataaga
    11641 tcacgaaaga aacattcaaa tttatttctc caacttctga aattataaat agtcctctgg
    11701 ggactttgat aaaaaattgg aagcttccac cgtactaaac ttcagaagtc agtaaataac
    11761 aagcagttaa gaatataatc atggctgatt tttttgtcga tgtctgatcg ttggcgctaa
    11821 aaaaagttcg tgattgcagg tatcttcaat ttcaggtata gccgaaaata aagagggctt
    11881 aaaccatttg catttgaatt aaaccttggc cggacgtacc gctatttata ccgttgcccc
    11941 cttattttag tcgttttatc acggttgttt taaaatcaaa acctccaatc tcaaactgct
    12001 ctatttcaaa ttgttgatgg tcaaggtatc gttcgataaa aggaagatcg ggcgcatagt
    12061 gaaatttccc tccggttttc agcgatttga gtatgtccat atatttccgg gcatattcca
    12121 tatagttacc gtcgtcgcgc agattgtggt gtttaaagtg atttgaaaac ccaaggttgc
    12181 tggttatggt tccccacttt tcggttccgt aagcaaattc cagccagtca gaatttatca
    12241 gggtagcttc cggaaatgta aatcggtcga ttccggtcgc atcaaaacca agctgacgaa
    12301 aatagccgac cagggttccg tgtttgccgc atccgatatc cagaacaggg tttgtaagtg
    12361 tacggatatc aaggtgaagg atatccagtt gtaattcggc cccgtattcg gagcaggcga
    12421 caggatttat atccctgtca gcgccggcgt aaattttttc ggcaaacgga ttggttttct
    12481 gcagccatcg cttcaggttt tcataatgat ctttttcaat cgcttctaac ggactcttcc
    12541 cggcctttat gactgagaat aatttactgt aaatttcccg caggtcattt ttagcctggg
    12601 ggttgaaggt gtaatactgg ttgatcctgc aaaattcctc tatggcctta tcggtggcat
    12661 aatctgtcag gatgttttca tcgccggggt ttaattctcc tatattcgaa atggctttta
    12721 tggtttcatc aacaaaccgt agtttcgttt cgttatcatc aagaaatacg tttttcccct
    12781 gattaaattc aatctgttta tcaatactct ttaaatgttc atccatttgc agcagttctt
    12841 ttgtatgcgg cacaaccggt atagatcctc acaaacttac cctttttatg gtttcattcc
    12901 cggccgggaa taagcgcgtg gttcacgggg cgggacaggg aacaccgtat gcctgtgatc
    12961 ctgtttaccg gcagcgggga tttttacatc cgcggacctg tacgacccat tatttgccgg
    13021 gattataaac gtaaaacgtg tgctgaatgg tagtatcctt cgtcatgcct tttgcgggga
    13081 caaatttcgg caatttcttc agcgttttta acgcatcctc ctcaatgccg ggggtttgac
    13141 agtcataacc cgatctttta gcctgggcag tatccagact tacgctgaga atattgccgt
    13201 ttttgtcgca gtgtatcagg caagggatcc aacaattgat tgccgttttt tcagcagcgt
    13261 cgttcagctt aagttctgat gaaaagaatt gcgacagatc ttcactaaac gccgcgttgt
    13321 tattcatatc gaactgacag gcctcctttg ttttcgtcat ctcctgaaat tcaggcttgc
    13381 cgatgattcc ggccagtagt gcgaccggga tgaccagcac gcctcccaga ttggccccga
    13441 tatgcaattt tacagagtat ttgatatgat gatatttact gcgcaaaaat ccggataata
    13501 gtccgatgga taacgccagc atcacgatga agacaagctg caacggagcc gccgttccga
    13561 gataaaagag atgcaaagcg gcaaaaatga gacttgtgat cacgatcggg aaccaggtcg
    13621 ttacatttct gttttgtcgg gagaaaaagc ctgctttgat aaggtcttgt atatatcctc
    13681 tgaataaaac ttcttcgatg acgggtgcgc cggccactac caggaaaatg ttttgaaaaa
    13741 ggttcatatc cctgccaccc aggagattat tgaaacctga ttttccgatc agtaaaaaag
    13801 tcagatttaa aaggaggctc aacagaagtc cggacaggag cgggaacttc tttaataagg
    13861 aaccctctcc ggtatccgcg tagctgaaaa aatgatctct cagactgagc ccaagaacaa
    13921 accgcaatac cagaataata agagtaaggc ataccagata acttaccatt tcggcataga
    13981 aaaatccgac cggcaataat cggtgtccaa acaagatcac aaggatgcac acgaaaccga
    14041 cgatcagtgc aatgtaattc cgggttttaa agtcattcat ttttcagggg tattaatcgt
    14101 tgcatttttt gcggcgaata agccggttat tcgttgcagg gctggttctt tagcccgggc
    14161 gaaatccggg ccggagggca acggctgtaa gtcccgggcc tgtttttatt ttctttttcc
    14221 gggatatcat gcatgtaaac gctgccggca ggttttggcc ggcaattatt tggttttata
    14281 atctgcaaga ggttcggccg ctaagccaaa atcctgttcg tttggggcgt ttagagcttc
    14341 gccttggtgc ggtccttccc cccagacttt tgcaatcctg tcttttattt tttgctcaaa
    14401 cagctcaatc agttgcttat tggcattgac caattcctgt tctttttcga tgcgggaaac
    14461 gatttggcgt tggatatcga tggcaggtag tggaattaaa gttgatttga taaaatctgg
    14521 cggtacacgc tgaagtccgc cagttccagt catattcagt ttcccttttt ctctgaagct
    14581 atttgtgata acattaagat aaatccatgt gggaagaatt ttatcatttg gtcgcaaaac
    14641 aaaatattca cttgaaccaa accctatacc gttaactaaa ccctttgcaa ttccagcttt
    14701 cccattttcg aaacaaggag ttacttttgc taacaaaaca tcatcttctt taaagtaagt
    14761 atatcctttt atgacttctc ctaatggctt gacttgttta gggtgaaaac ttatttcatt
    14821 ctcgtttata tctgccatag gcacaaaaga aacttctatt tttctggaga cattagctaa
    14881 ttctgatttt ttaggattta cctcgcaaac ctctcctaac tccaccattt cccattccgg
    14941 gtcatcccga tagctatcaa tatcaatttt aggtttgtag ttttccacta ccatttttgc
    15001 cccatcaatt attttttggt agccttctat ttcggcgaca atttcttcct gaacggagag
    15061 gggggggagg gggatttgta tagattttat atctgcaact cttagactag cttgattaac
    15121 tgctttttga gcaaatgttt ttgctttatc aataaaaaat tcagatttaa atatcgccgc
    15181 agcatattcg ggaacaattt tatttttgtt aggcttaaac cttattaaat tagttccatg
    15241 aactactttt tcagatatac ctttaaagat tgctgatttg gctaaatgtt caaaactatt
    15301 aatatgactt aacaggatat ccccttcttc cataaaatag tcctctggta cttcatcatt
    15361 agtccacttg gttttttcta aatcaactgt tttatcagca atagtttgaa ttcttgtaac
    15421 tctataattt ccaacttcat caatttgttc gacatttcga ccgttcctaa ttgattcgaa
    15481 taattcttct atgggtttta atggataagc agtgttagaa attatttgtg ttttatacct
    15541 ctccccgctc aaattataat ccccgttttt accgatttcg ggtttgggaa cggtgtgggc
    15601 gattgggttt tggggttttg cggggggcgt ggttgttttt gtagggacgc gattattcgc
    15661 gtccctgcaa aaacaacgaa aatcgttaat aattgtaatg gcatccttca attgtccgcc
    15721 tttgatcggg ttgcgctggg cgcccaaccc atatccatcg ttgtcgattt ttacaaataa
    15781 aatttcatca cgggttttgg ccaattcctt atccaataat aaaatggagg tttttacgcc
    15841 gctgtaagga ttaaaaaccc ctgcgggcaa gcttaccacg gcatacaggt aattttcgac
    15901 cagcattttc cgtaaatcct tgtaagctgt gccgctttgg aatatgaccc cttcggggac
    15961 tataatgccc gcccggcctt tggggttcag gtgttcggca atgtaatcga caaacaacac
    16021 ttcgctgcgg ctgctgctga tcgaaaattt tttgtggggg cgtattccac ccttcggact
    16081 cataaagggc ggattggcca ggattacatc gaaggtatcg ttccagcgtt cctcgctggt
    16141 aagggtgtcg tattcgtgta tgtgcgggtc ggagaatccg tgcagataca aattcaccaa
    16201 cgacaggcgt accatgtcgg gcgaaatatc gtaaccggtg aaattaccga tgatgcggcg
    16261 gcgttcgtcg ggtgtgatgg cgtccttgcc atccatgata tgtttaaatg ccgaaatcag
    16321 aaacccggcg gttccgcagg cggggtccag aatggtatcg gttttttgcg gatctaccac
    16381 cgcaaccaga aaatcgataa tatgccgtgg cgtgcgaaat tgtccggcat cgccctgcga
    16441 gcccatcacc gaaagaaggt attcaaaagc atcgcccagc ttttcgctgt gggtgtattc
    16501 gaattcgccg atggtattca ggaataattt caacgttacg gggtcgcgat agggtaaaaa
    16561 ggcatttttg aaaatatcac ggaacaactg cgggatgttg gggtttttgt ccattgccgc
    16621 aatcgcatcg gaatacaaca tcagcatttc attggctgtt actttggtgt cgaaaaggtt
    16681 attccagcta tatttttcaa attcaaccag tattgtcttt ccaggttctt tggggtccgg
    16741 tatcgtataa ttcgaaaaga attttgcttt accacctaat tcaatggctt ccttgtccat
    16801 gtcgtccatg aacttgtaaa ttaatgcgat ggtaatctgc tcaatctggg cttttgggtc
    16861 gggtaatttg cccaccaaaa tatcacggca atcgtcaatt cttttcttcg ttggggtgtc
    16921 taacattatt gatttttttg atttttgtgt attgtaagga cgcgattaat cgcgtcctta
    16981 caatatcgta ccatcatgaa gtttatcgtc cgcccatttc gacggattgt ttacaatata
    17041 ttttgaaatt cgttgaaacg acatatcgtc tcggataata tggtcgtaga aacgggattg
    17101 ccaggcgaaa ccggcgtgga ttttacgtaa ttcaaatgaa acacgccctt tgtaccatcg
    17161 cacaattcgc gacaaattgt tgtttaacat cgggttttta ttcccggcaa atccaccaat
    17221 atgggtttcg gattccattt cggattccat ttcggattcg gatatggtgt cggtatccgg
    17281 atcggtatcc ggatcggttt ccatttcgga tttcgtttcc gtttcgaatc cggttttgaa
    17341 tccggtttcg gttcccgtgg tggtatccgt cgcggtttcc gtggtggttc ccgtcgcggt
    17401 ttccgtggtg gtagagacgc gattaatcgc gtctctacca ccacggccgt catcatcacg
    17461 accatcaccg gaaaccggtt tatcgatgac gattattcca tgtacatgat ttggcattac
    17521 aacaaattca cccaattcaa caaatgggaa atggattggt atttcgttcc agatttgatt
    17581 tgcgattttt cccaattccg ataattccat ttttacgatg ggcgtttcaa caattttccc
    17641 aaaataattt tcccgatttt gggtgcagat ggtcacaaaa tacgctgcat tggagccata
    17701 atcccaattt tgtagtcgcg ccgatggtat ccggtattta ttctggaatt tgtctgacat
    17761 attacgccgc aaatctatcc aaattaatgt aatctttaat gtacagcgga atcacttccc
    17821 tgtatttgac tgccacttct ttgaattgcg agatggttag cgtcggattg gtttgtaatg
    17881 cgtgaaagtc tttggcttcg ataattttcc gtatttcgcc gtcaacaata taggctttga
    17941 aaaaatactt taaagcccgc aaattaacat cttcttcggg cggatagatt gaaatgaatt
    18001 tgtcgaattc ttcttccagt aattcgtcct tcgatttgaa tttgggaatt ataccaaata
    18061 ccttttcaac catttccctt atcgaaactt tacggtcaat ctttgcagct ttccgcaatt
    18121 tttcaaggct aaagtattct tcaggtttat caaaaatttc gttttgaata tgagaaacta
    18181 cctgttccca gttgccaagt tccacattct ttttaatgat gtcgtccatt acaatgcggt
    18241 cttcgaactt tttaaacagc atccggtcaa ttttcattcc ttcgtatcct atttgttgct
    18301 cgtttagaac cgccagcggg tcgggcctaa aactggtgta tttatcaata tcaacacctg
    18361 tgccgccacc ttcgccctca ccatttcgtg gtttcggcag ttttaatttt tcatcgtaat
    18421 cgaatttttc ttcaaaatat tcgcaattgg caaagaaatc gaatagttta aagaactcct
    18481 tttcgtgttt tacatgttct atttcgccca gttcgttttt atgccggaat tcgaaggtgg
    18541 tttttcgagt cccgcgccct tttatctgaa caaagtccga agggctgaaa acgggtcgca
    18601 tcatacaaag attcagcaaa tcggggcaat cgtatcctgt tgtcatcatt ccaaccgtaa
    18661 cacaaatgcg tgttttaccc gatttgtagc cttccagcca ggttgtttta ccattcaaat
    18721 tattgttggt aaaattgata gtcatttgct gggcctcgcc aacctgcgaa gtaacctgta
    18781 cagcgaaatc ggaattgtat ttatcgggaa aaagctgttc agcgaattca tttagaattt
    18841 ctgtcagctt tcgggcatgg ttttgactaa ccgcaaagat gatggttttg ccaatttcgt
    18901 tggtaattgg atcacgcaag gcattttcca aaaaggtatg gcaaaatacc cgattggttt
    18961 tatccgaaaa gaatttgcgt tcaaaatcac gagaaataaa agtttcagtt tcttcgcctt
    19021 cttcggtggt tatggcaacg gcataacctt catcggaaag caattgcgtg gttatctcgg
    19081 ttcgggcatc gagaacacgg ggattaacca aaaatccatc tttcacacca tcgagcaacg
    19141 aataacggaa cgttgggtcg ccaccctcgc aaccgaaagt tgcataggtg tctcttaaca
    19201 ttcttctttc aatttcccgc ggatcgtttt cttttacttt gtcaggatca acgcctttca
    19261 gataatcctt tggtgtggcc gttaaaccta atttgtagcc ataaaaatac tcaaacacgg
    19321 ctcgcgcatt tcccgaaatg gagcgatgcg attcgtcaga gattattaaa tcaaaatctg
    19381 ttggcgaaaa ttggctgcgg tatttattat cgaacatcag cgattgaatg gtagtaacca
    19441 caatatcggc tttccgccaa tcgcttttat gttctttaaa aataaaggtt gtataatcag
    19501 gtttcagata gttggtaaag gctttccacg cctggtcttc gagctccagc ctatccacaa
    19561 ggaataaaac tttccgagcg ttttttgtac gtaaaaacaa ccggataaca gccgcagcag
    19621 taagggtttt tccagtgcct gtcgccattt caaaaagaaa gcggtcttta ccttcccgta
    19681 ccgctttttg caaatgcaaa atcgcactta gctgataagg ccttaaaaac cgaagtccat
    19741 tcgtccggat gaaatcctta cttgtagaaa tgctgccgtt ccaccctggt cgttgagcat
    19801 aatcaggcat ttgaacggta acaatataat caccccccac aggttctgtt gctaatgaat
    19861 ttctgtcggg gttccagtct ttaatggctc caatctcctt tggcgaagga aatttataaa
    19921 tcggtttggg atttcctttt ttcaaatccc acaagtaatg tactatcccg tttgaaagga
    19981 ttatgaactg tgtaccgact atattcgcat attttcgtgc ttgttccttt gccaccaaag
    20041 ggtgcaggct ttcccttttt gcttcgagta cgcaaatcgg tttattgtcg gtatctacaa
    20101 gtaaaaaatc tagcgaaccc gattttactt tttcgtaatc attaccccag gcgtcaatct
    20161 ctttttgagt gatttttaca tggttttcaa gtaaaatatt agctttttct tcatgattat
    20221 cgaagaatct ccaacctgct tcatcgagta gttggtttat cttaatcctt gcatgtgcct
    20281 cgttttgtaa catttatcgt atcagaaaat aaaattgtta aaatatccat cgtatcagta
    20341 tcgtgcgggt gcggcgttaa aaaatcaggt cttttgcaaa atttggttat ccgttggctt
    20401 cccgaaataa cgggataagt cgtgcgaaat attccgataa atatcgggat gttgttttac
    20461 tcacaagatt atttaattta aaaggtattc gcgaggactt cgccgttgtg tgttggctgt
    20521 ttccatttta ttgtcttttt gtagcataat ttccgctgtc gttggttttg ctttgtttgt
    20581 gcctagtaaa atatatcatc aaaaacttac cccttttaac cctcccggga gtttgcacgc
    20641 acccttgtcg cctccgtttc tccgttccct caggcggact gcaaatcagc attctaaagt
    20701 agaaaagaaa tctgtatgtt tttacttctc tttgaaaata attgaacaat ttataaatta
    20761 ctaaataatt aaaagtcgta ccgggcatga ttatttctgt tctccggttg ctgacggggg
    20821 tcccgggaat cctttacatc gtatttggat gggtaataac gtaaagagga gagatcacac
    20881 cagccggtag cccacgcccg gttcggtgat cagcatctcc gggatggaag gattggcttc
    20941 gatttttctt ctcagttgcg ccacgtgtac ccggagggtc tgggcgttat cggcataggc
    21001 gttgccccag agttcccgga tgatgtaagc atgggtcagc actttcccgg cattttttac
    21061 catcaggctg aggaggttat attccagcgc cgttagccgg acctcttcgt tgttgacttt
    21121 aacgatgcgg ttggtgaggt ccaccgaaag cctgccccgg gtaaaaacag gcgattcgtc
    21181 gatccgcagc gaatggcgga tggcgactct gatacgggcc agcagttcac cggtgttgaa
    21241 cggcttggtg acatagtcgt cggcgcccga atccagggct ttgatcatca ccttttcttc
    21301 gtcccttacc gtcaggataa tgacgggcac ggccgaccat tccctgagcc tttccagcaa
    21361 ttgcaaaccg tccatatcgg gcaggccgag atcgagcagt atgacatcgg gatgctccat
    21421 ggccgacagg agaaccccgt tcttcccgtt ttccgactcg atgacatgat aatcgttggc
    21481 ttcgagcgta atgcgaagca ggcggcggat ttgcacctcg tcgtcgatga tcagtacttt
    21541 cggtttactg gctaatgttt ccatttactc tgcaattggc aattggatgg cgaccgataa
    21601 accgccttgg ggcctgttta cggcttttat cgtcccgttg tgcgcttcaa ccagtgcctt
    21661 ggcaatggtg aggcccagtc ccgttccccc gcttcgggaa ccgggaacgc ggtaaaactt
    21721 atcaaaaaga tgatcgaggg aagctgtcgg aacgccgggg ccggcatcct cgatttttaa
    21781 gaccgccgtt tcgccgtcag ccgatgcgga aatgcttatt tcactgcccc ggggcgtgta
    21841 gatggccgaa tttcgcagga tgttcatcag ggcctgtttc atccagttca tatcaatttt
    21901 tacggcaggc aacagatcgg ggatatcaac cctgacagca tggtcggaca gttcatcttt
    21961 catatggtta accactacac ccaccaggtc gcaaatatcg catggctgca aattaaggct
    22021 caggtatccc gaatcgatct tcgacatatc caggatattt tcaatgagct ggttcagccg
    22081 ccgtgccgac gcttccaact cggtgcagat gctgttcctg ttcacgggat cggcggccgt
    22141 tttatcgtcc atcaggttcg aaacggcgct gctgatgatt gaaacagggg tgcggagttc
    22201 atgcgaaacg ctgttcagaa ccgtctggaa gagtttctgg gattcctggt acacctggtt
    22261 ttcctttgct ttatcgatgc tgatctccct ttcgagcgct gaagtgatct gcgtgatgaa
    22321 agatttcaga aaaataagct tttcttcgtc gatgccggtt ccggttttcg ttgctatgcc
    22381 gatgactccc agggttccgc tttgcgccga taacgggaaa tagcggatgc tcagggtatc
    22441 gttcaggcgt cttaccacgg cgcgttcttt catctggaac acgaggcagg cgtattcaaa
    22501 ttcagtttca ctgaagaaac catcatttcc gaaagcccgc ttctccagtg aaggaccctg
    22561 cttttccctg aggtagacga tcgcgtcggc atcaaaatgc ctgtcaagtt ccgcccttac
    22621 tttgccgatg acgcctttga tggagttggc attgttgagc gattcgagca gcgaatacag
    22681 caacgtgata tttttccggc tgagttcaag atcggcctgg tttttccgca ggcggttgat
    22741 cagcgatcct ccggttattg ccacagcgag gttggcaaag agcatgatca tatcgtggaa
    22801 gctggcgata tgaaaggtaa gaattggcgg tatgaagaaa aaattccagg ccccgaagtt
    22861 gaggatggct gtaaagataa cggccccgcg gccaagaaaa agcgatagga tggcagtaag
    22921 gatcagaaga atcagcccta cggtctggta accgatatac tcttttaccg ggaaacaggc
    22981 tgcggaggtt attacaacag ccattgccgc atagaggtat tccttcggcc tggtgaaaat
    23041 tttaatattc gcggcattga ttttttgtgc ttctttcttc attttaatct gggttatttc
    23101 tacatctgga ttctttttct gaagccgtct gatcagtaaa ccggtatttt cccccaaagt
    23161 gatttttacc ggtttaaagt taccaataag caggcggttt acgctatacc tgtcaataag
    23221 atttgtaacg ccatccacta catcataatc gagcaggtga atgaccctgg cgccgctgag
    23281 caatgcctgg gtaaggttat tgctgaattg tttccggacg gcttttccgg ctcctggccc
    23341 gggttctgcg caaaccacaa accattcgcc gtcgctgtcg gcatattgct gcctgatttc
    23401 ctgcaggaac gatccggcag atggctcaga acccagaagg atcatcagcc tgcggccagg
    23461 ggtgccggtg cctgagcttt gacgctctga agttttcata acacatcggg tgtttaacct
    23521 gctttgaaat tgatgaccgc aaatccctct gatgaagaag atttattgaa tccatcagac
    23581 agggattcac gatgcaagat tacgatgact tttattaatt agaaatcaag attccggcct
    23641 gttctataaa gattttatta agtgcgcttc atcattcggt ccgaagacaa gttgccggat
    23701 tatcgcatct ttgtcaacga acgccatgcc tttagaagtt tataaaatct ttatccgaac
    23761 cgggcagaaa ttaatatttg ccttataaag cccgtcataa ttttgtcccc tgaaagccgt
    23821 cggcgcccgg ttggcgggcg caaacaggaa gatgaaaacc gtttgtgcgg ttcaatttta
    23881 ttcctgacgg ctaagcgcat cgagaaaatg aaaaatgatt ttaagttacc atcggatcaa
    23941 cagctcaggg agttcctcat cgggaaggca aggagattat cggattccgg cacgtttcac
    24001 aaaatctccc ttattgcatt cctggcctgg ataggcctcg gcaccgacgg catttcatcg
    24061 tcggcctacg gccccgaaga ggcgtttcgt tacctggggc agcatcatga cctggcactt
    24121 tttgtggctt tggggaccat ctttacgatc gtcatcatca gcacgagtta taaacagatc
    24181 atccggcttt tccctcacgg gggaggcggt tacctggttg ccagcaagtt attgtcgccg
    24241 accgtcggga tgatctcggg cagcgccctc ctgatcgatt acctgctcac gatcacgctg
    24301 tcgatctcga gcggagcgga cgctgttttc agtttcctgc cgccgcgtta tcatagcctg
    24361 aaacttattg tcgctgtgct tgcccttgtc ctgcttattc tcatcaatat gcgcggggtc
    24421 aaggaatcgg ttctgtcgct gacgccgata tttgtccttt tcatggcgac gcatgttttt
    24481 atcatcctgt atattttctt ctcgaattcg gcggcgatgg ccggcgtggt ggctgaaagc
    24541 gctgaaggct tcggcagttc ggtttcacag ctcggtttcg ttgccaccat gttcatcatc
    24601 ctgaaagcat acagtatggg cgccggtacc tttaccggca tcgaagccgt cagtaacggc
    24661 ctgcccattc tgcgcgagcc caggcctgta acggcccgca agacgatgaa cctcatggcg
    24721 ctgtcgcttt ccttcctggt tttcggtctg ctcatgggat attccctgta ccaggtgagg
    24781 gccgatccga tgaaaacgct gaacgctgtg ctgcttgaat cgactactgc cgggtggaac
    24841 aggcttgccg gttattcttt tgtcaccgtc accttgtttt ccgaagcggc gttgctgttt
    24901 gtagcggccc agaccggatt cctcgacggg ccaaggatca tggcgaatat ggcggccgat
    24961 caatggctgc ccaagcgttt tgcttcctta agcgacc
//
LOCUS       DAOPYV010000043        24341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_2067_length_24341_cov_13.757062, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000043 DAOPYV010000000
VERSION     DAOPYV010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24341
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2067_length_24341_cov_13.757062"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..712)
                     /locus_tag="PKI35_07130"
     CDS             complement(62..712)
                     /locus_tag="PKI35_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNX44193.1"
                     /translation="MKKYLVILLICIGSTNLFSQIVSVTPEAGIVFSGYRNDNIIHDD
                     GIKIGGMTGVTADYRLTDALCARSGLMLDQKGNYFVMPLSDQFGTPLGELRSFLTKTY
                     VGVPVLLRVKLYKELYFEGGGYVSYLIRSKIVDINDFNDKNDTTVKHIENLGSLKFSD
                     RMNKGLYAALGVSIPLFGTQGLQVSVKYDYPLVFNRSREKYYAFCLSVGYRFKIRD"
     gene            1058..1582
                     /locus_tag="PKI35_07135"
     CDS             1058..1582
                     /locus_tag="PKI35_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNX44194.1"
                     /translation="MNPLKEFIIPFVGLELGNHEFDFEAGDKFFEQFEYTQIHSARLN
                     VTVTLEKQERMLVFNTRIKGDIGTVCDRCGGDFRLPVEGSERLIVKFGEQYAEENDEV
                     VIIPDSEYKFDLAPYIYEYAHLMLPARIVHPDDADGNPACDPEMLKRLSDLAPHESAD
                     PRWEALKNLHPEKE"
     gene            1641..1826
                     /gene="rpmF"
                     /locus_tag="PKI35_07140"
     CDS             1641..1826
                     /gene="rpmF"
                     /locus_tag="PKI35_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNX44195.1"
                     /translation="MPNPKHRFSKTRTAKRRTHYKIDAPTVAICSNCGASVLYHRVCP
                     ECGYYRGRLAIEKSATA"
     gene            1876..2850
                     /gene="plsX"
                     /locus_tag="PKI35_07145"
     CDS             1876..2850
                     /gene="plsX"
                     /locus_tag="PKI35_07145"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNX44196.1"
                     /translation="MKIGLDVMGGDFAPGAVIGGAILALKELPSSDRIVLIGPGEVIS
                     AHLSDAGISPDLFDIVHAPDVIGMSEHPTKALSRKPASTIGVGFNMLKHKQLDAFAGA
                     GNSGAMLVGSIFSVNSIQGIIRPATPAFVPQENGGISVLIDVGTNPDARPDAMQQFAL
                     LGSLFASHVLKIENPRVGLLNIGEEEEKGNLTAQAVYHLLKGWEEINFIGNVEGRDLL
                     RNKADVVVCDGFTGNIVLKSIESMYRIMAKRKLLDDYFSRFNYENYGGTPILGINGIV
                     IMGHGISNDIAIKNMILMGRDIYESRLVAKIKHSLVDIAGKTNMASAL"
     gene            2974..3972
                     /locus_tag="PKI35_07150"
     CDS             2974..3972
                     /locus_tag="PKI35_07150"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNX44197.1"
                     /translation="MTKIRAAITGVAGYLPEYILNNEELSRMVDTTEEWIMTRVGIRE
                     RHILKEPGKGSSDMGAEAVKKLLAKTNTSPDDIDLLICPTVTPDHLFPATANIISHKT
                     GIKNAFSFDINAACCGFMYALQTASQYIETGYRKKIIVVGAEKMSAITDYTDRTTCPL
                     FGDAAAAVLLEPTEDEVGIMDAILHSDGVGFTHLHMKAGGSVKPPSHETVDAREHYIY
                     QEGQAVFKWAVSKMADVSVEMMHKHNLTPDNLAWLVPHQANLRIIDATGNRMGLPPEK
                     VMVNIQKYGNTTSATIPLCLWELENKLHKGDNLILAAFGGGFTWGSIWLKWAYDPK"
     gene            4167..4820
                     /locus_tag="PKI35_07155"
     CDS             4167..4820
                     /locus_tag="PKI35_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNX44198.1"
                     /translation="MKLLRNILLKRLSLAVGIILFLSVTAAAQHPKNTAYVEKYNSMA
                     VALMHEYQIPASVILGVAMVESGSGTSLLSRKFHNHFGVMGKNTHAVKKLGKHSKYRE
                     FESDTASFRYFCELMSRKQFYRELVKTYDHKQWVKAIRKSGYASAASHWQEHVLTAIE
                     KNNLTCYDTPEPDPLNYLPRDINGVKTNGMLVNSCFVPVKKTVSPIPASTSNLKASK"
     gene            complement(4871..5569)
                     /locus_tag="PKI35_07160"
                     /pseudo
     CDS             complement(4871..5569)
                     /locus_tag="PKI35_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
     gene            complement(5576..6271)
                     /locus_tag="PKI35_07165"
     CDS             complement(5576..6271)
                     /locus_tag="PKI35_07165"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNX44199.1"
                     /translation="MKKALILLLLAIAGIGVTAQTVQKSFLDQPYIEVTGTAELEVVP
                     DMIYLAIVLNESDTKGRISLEMLESRMKGRLTALGINVSGDLTVEDLSSSYMQYFLKD
                     KVAGSKHFSLLVHDAATAGKVIKALSEEEISNISITKIDHSQMAQKRFDLRLEAVKAA
                     KRKAEAMAECIGQQIGKAIYITENTAADAFVQSAVNSVLFESRVRSDQNQPEPAFKNI
                     TIKSGVSVTFILY"
     gene            complement(6317..7540)
                     /locus_tag="PKI35_07170"
     CDS             complement(6317..7540)
                     /locus_tag="PKI35_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNX44200.1"
                     /translation="MSPVGSYESLMAAIQGGAGSVYFGVGKLNMRARSSVNFTLGDVS
                     KITRICRQYKVKSYLTLNTIVYDEEIEEMKAIVDAAKEHKITAIIASDLAVLGYARQQ
                     GVEIHISTQCNITNIEAVKFYSRFADVMVTARELTLDQVAAITRQIEEQDIRGPKGER
                     VKIEIFVHGALCMAVSGKCYLSLHNHNSSANRGSCLQDCRRRYIVHDRESETELEIDN
                     EYIMSPKDLKTIHLLDRVLAAGVKVLKIEGRGRSPEYVKTVTRCYREAVDTYYSGNYT
                     PENIARWDKELATVYNRGFWEGYYLGRKLGEWSPVHGSLATQKKIYIGIVANYFAKSG
                     IAHIRMDTQELNTGDRVQIVGPTTGVYEAVIDTMVNDDKTVEKVVKGDNPTFRVSRTV
                     RRNDKVYRVVEAENK"
     gene            7945..9567
                     /gene="lnt"
                     /locus_tag="PKI35_07175"
     CDS             7945..9567
                     /gene="lnt"
                     /locus_tag="PKI35_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNX44201.1"
                     /translation="MKRITKILLAVLTGLLMAFGWPEHGFPLLLFIGFVPILLVEHDQ
                     WKKRKENSWFGIFGYAYLAFLVWNALTTYWIYNSTLVGGIAAIMLNTLFMTIVFTLFH
                     ITRRTMIGGNGGYLALIGYWLSFEYLHLRWDLNWPWLNLGHGFAAYNKWIQWYEYTGV
                     FGGSLWILLVNILLLMIIRDVLDKKSSIRKQALPLLGALLLIALPVLLSLIMYNRYRE
                     KQAPVDVVVVQPNLDPYSEQYEMAPSDVLDRMLRLAMTKADNNTQFVVCPESALQEYV
                     FEDRFEESASLKALRQFNQSYRKIAFVSGISTRKVFETGEPLSMTARKFKDVDRYYDA
                     YNTALYVKHDGTFQKYHKSKLTPGVEKMPYPRLFKFLENYAIDLGGTVGSLGIDADQI
                     PFSVNDSLKIAPCICYESAYGEFMSNFVRNGANLIFVITNDGWWGNTPGHRQHLLFSS
                     VMAIETRRSVARSANTGISCFINQRGDIMQRTAYWTPTAIRQQINANTGLTFYVKYGD
                     YIGRLGILVAALMALLTLTFSLRSRARSLHAR"
     gene            9788..12199
                     /locus_tag="PKI35_07180"
     CDS             9788..12199
                     /locus_tag="PKI35_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNX44202.1"
                     /translation="MKYSLEKPLSTPEIENPDTLYGFITFASRYFMSANQWDENLGFV
                     LDRLQAVTTAGRICLFSADSLEKYDSKEDIRVWSCSKRKQQSRFSAAPALLTESLIKW
                     IPALRQNRVIRSDGNSINPAAGKLTECPSLIIIPVFSGNDWWGALTLEFSADAYRQKR
                     MEHRIIRFFCQMMGSAIHRQRLIDAIKVDKSYFEELFESSPFGIAVLNLDGELQHINR
                     GFTNIFGYDEKDLAGKKLDDILPAPEERENARELTERSLAGEEVSFETIRYRKDGAPL
                     FVSVLVRQLHPTPFESSVYSIYIDITERVKTQQQLTENKTKYQSLFETSSDGICIFRK
                     GLLIEANHRFYEMLGCRPDQVIGSSIFNFSPEFQPDGSPSIMKGRSYYRKALSGETAF
                     FEWLHQRPDGSRFFGEVILTRFELFGQLYAQGILRDISRRKATEEHLQKRYEFINFLS
                     KASADLINLDPAHIDKAITEALMYSAKFANCGRGYVYLIDPVKNTSDLAFEWEDSNIV
                     NTNSLIRRLAMDQYAPLQELLNTRQVLQVQKSQLNGQDDALHIGELFDSLGIKALIII
                     PMLVDNHYTGFLGFHSSAEMSWPEETLNTLRLSGQIIANAIDRKRVNRELSEAKQKAE
                     ESDRLKTAFLASMSHEIRTPMNHIIGFIELLKDPSLSETERNEFLSIIRNSGNHLLSL
                     IDDIIDIAKIESGQMELHPVELSLNDFLNDLYLAFREQFHKSPKSNIHFRLDLPATPE
                     NCYIQTDTMRLRQVITNLLSNAIKFTHNGHIRFGFEKQDEHTLRFFVEDTGIGIPKEK
                     TTDNF"
     gene            12144..12791
                     /locus_tag="PKI35_07185"
     CDS             12144..12791
                     /locus_tag="PKI35_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX44203.1"
                     /translation="MWKIPASAFRRKKQQTIFERFRQLDYSYSREYGGTGLGLAISKG
                     LVELLGGEIGVKSEPSVGSTFYFTIPYIPLSAVQQQAAVSAKTSGKYNFEGKTILIVE
                     DDEINYHFLNIILERTGAQILHAADGQKAIDMVKANHVDLILMDIRIPVIDGYTATRM
                     VKNLRPDLPVIAQTAHALDDEKNLCIEAGANDYMAKPINRKQLLLKISRYLINEE"
     gene            12892..13701
                     /locus_tag="PKI35_07190"
     CDS             12892..13701
                     /locus_tag="PKI35_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNX44204.1"
                     /translation="MEKTKVLFVSQDITPYLKDSPMGHISRHLPQGIQERGREIRTFM
                     PKFGNINERRNQLHEVIRLSGMNLIIDDTDNPLIIKVASIQSARMQIYFIDNEDYFQR
                     KYVFRDKNNVFFEDNDERAIFFSRGVLETVKKLGWAPDLVHCHGWMTSFMPLYIKKAY
                     HDNPLLSNSKVVLSVYDDQFDETLNNNIGNKIKHEGITAKDLKHFKKPTFANIMKAGI
                     DFADGIIVASPEINAEVHEYALNSGKPVLDYQPEETYIDAYNEFYDRILSA"
     gene            13750..15066
                     /locus_tag="PKI35_07195"
     CDS             13750..15066
                     /locus_tag="PKI35_07195"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HNX44205.1"
                     /translation="MKLFRNAFLYLILASPAVLFTTCTKDPDLVGLNLVSPSELLKLG
                     YTDTAQIVAYTVKVDTVSTQKLTYALIGSMNDPVFGQTDATFYSQILLVDEGSDYGTS
                     PVFDSAYLVLPYHGVYGDTMSNMTFRIYELTQPLDATDTMYSFNTVPYDESHLLGSLT
                     FVPRPHDSVYIDGTKTAPSLRIPLTEYFGQRMLSISADSLDDNDSFLNCFYGICIVAE
                     KQETPGKGSILYFTVPSSKSMITLNYHNTEDTTKAYYIINTNCMRFGNYSHNGYSSAS
                     PLLQQQFSGDTTVGNQYLFLQSMGGCRVRLRFPSVPDWQSKHHLIVNDAQLIFTNASP
                     GSQYTEPASLALMQIDDDNVSIGDYLDDQLESSTYFDGNYNETDHTYRFRVTRYIQQL
                     MTGKVENNGLYLIISGAAINSDRLILNGPQNPYGKLKLFVKYTVLD"
     gene            15400..17238
                     /gene="glmS"
                     /locus_tag="PKI35_07200"
     CDS             15400..17238
                     /gene="glmS"
                     /locus_tag="PKI35_07200"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNX44206.1"
                     /translation="MCGIVGYTGYRKASEVLIPGLKRLEYRGYDSAGIALLNGELSVF
                     KTAGKVADLESLISSCDLEGSIGIAHTRWATHGEPTDANAHPHLSKSGKIAIIHNGII
                     ENYAVLKEELMNRGYTFNSETDTEVLAHLIEDVQLNEKCGLVEAVRLALGQVTGAYAI
                     AVLAEEEPDTLIAARKGSPLVLGIGEGEYFLASDAAAIVEYTRQVIYLNDYEIAIISR
                     ENGYRIITVSNTEKTPYIQTLEVSLSALEKGGFEHFMLKEIFEQPRTVRDSMRGRLNM
                     EQGSVILGGIEEYTRKLLNARRIIMLGCGTSWHAALVGEYLIEELARIPVEVEYASEF
                     RYRNPLISEDDLVIAISQSGETADTLAAIELAKSKGATILGICNVVGSSIARATHAGS
                     YTHAGPEIGVASTKAFTSQVTILTLMALMLAERKGTIAASRYYRLLHELNSVPEKIEK
                     VLQSNSRIREIAVKFSKKRNALYLGRGINFPVALEGALKLKEISYIHAEGYPAAEMKH
                     GPIALIDDEMPVVVIATRKGNYDKVISNIQEVKARKGKVLAIVSEGDETVCSMADHCI
                     AVPETDEMLVPLIATVPLQLLAYHVAVLRGCNIDQPRNLAKSVTVE"
     gene            17542..17952
                     /gene="mce"
                     /locus_tag="PKI35_07205"
     CDS             17542..17952
                     /gene="mce"
                     /locus_tag="PKI35_07205"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNX44207.1"
                     /translation="MNISHIEHIGIAVKNLEESILYWEKVFGLKCYSIEEVKDQKVKT
                     AFFMVGQTKIELLESTDPEGPIGKFIEKKGEGIHHMAFAVKDIENALTETEAKGIQLI
                     DKTPRQGAEGLQIGFLHPKSTFGVLTELCEDKNK"
     gene            18039..19598
                     /locus_tag="PKI35_07210"
     CDS             18039..19598
                     /locus_tag="PKI35_07210"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNX44208.1"
                     /translation="MAIENKIQHLLDKRDEAALGGGKKRIDAQHKKGKLTARERIDLL
                     LDEGSFEEFDMFVTHRCKDFGIENEKYLTDGVITGYGTIDGRIVYVFSQDFTVFGGSL
                     SEMFARKICKVMDQAMKMGAPLIGINDSGGARIQEGVQSLAGYAEIFERNILASGVIP
                     QLSAIFGPCAGGAVYSPALTDFIVMSKTNSYMFVTGPKVVKTVIGEVVNEDELGGAMV
                     HGAKSGVSHFVAEDEQEGIMLIRKLLSYLPQNNLEEPPLLPCDDPIDRLDDSLNYIIP
                     DNPNKPYDVKDIIHSIVDNGEFLEIARHFAPNIVIGYARMNGMSVGIVANQPNFLAGV
                     LDINASRKAARFVRFCDAFNIPILTLVDVPGFLPGTAQEYGGIIIHGAKLLYAYGEAT
                     VPKVTVTLRKSYGGAHDVMSSKQLRGDINYAWPSAEIAVMGPSGAIEVLYNKKISAVE
                     NQADREAMISKYENEYKDKFANPYDAASYGFIDDVIVPRTTRFRVIRAFQALATKKDV
                     NPAKKHGNLPL"
     gene            19610..19885
                     /locus_tag="PKI35_07215"
     CDS             19610..19885
                     /locus_tag="PKI35_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44209.1"
                     /translation="MSIFKKKDQNNGAEQPVKPEAVQLPEKKEVEPEIAAVIALAMHL
                     YDMQMREDENAIITIQRIMKPYSPWNSKIYGLRQQPLRIPGLRTRIK"
     gene            19907..20347
                     /locus_tag="PKI35_07220"
     CDS             19907..20347
                     /locus_tag="PKI35_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNX44210.1"
                     /translation="MKNLKFKIKDTEYNVDILSTDLNQAKVSVNGEVYDVEMEAPIKA
                     TKTPTLVRTLAVPDTNTTPSTARTAKPSAPKGTGAMKSPLPGKILDIFVKIGDSVKVG
                     QKVLCLEAMKMENNINSTRDGSVTAILVQKGDSVLEGDVLVEIN"
     gene            20370..21437
                     /locus_tag="PKI35_07225"
     CDS             20370..21437
                     /locus_tag="PKI35_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HNX44211.1"
                     /translation="MNSLTIFWVAFIVLFFLVFGIDLYVTDHRKGELSVKTSLRWTAV
                     WISIALLFGVAIYFFFPQNPESTVRTSSTMMFKFISGYLTEYSLSVDNLFVFIMIFSL
                     MAVHPKNQPKLLKLGILLSIVLRILFIVAGMELVQRFHWIIYIFGVILLWTAYKMAFT
                     SGDDQVDPKGNILYRTASKIFPLDPDPDANHFMRKMNGKWHITMMFLVFLVIGSTDVL
                     FAVDSIPAIIGVIKEGASQILTPQEEDFIAITSNVFAVMGLISLFFALKGIMGMFRFL
                     KHGVSFILFFIGAKMLLTSVPAVEHFFAAHSWVSLVVIISTLAISILLSMVIAEKEEI
                     EDLHEDLDEIQHALEDKDKNV"
     gene            21440..22588
                     /locus_tag="PKI35_07230"
     CDS             21440..22588
                     /locus_tag="PKI35_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNX44212.1"
                     /translation="MDIKELLPVLYSITWQNLVMIAVGLTLIYLAIAKEYEPTLLLPM
                     GFGAILVNIPLTSAITQIDPVTGQAIEGALSVFYQAGIATEVFPLLIFIAIGAMIDFS
                     PLFKNPFLLLFGAAAQFGIFFTMIAATLLGFDLKEAASIGIIGAADGPTSIFVSSRFA
                     PNLLGPISVAAYSYMALVPIIQPPVIRALTSRKERMIHMEGHAKSVSKSALIAFPIIV
                     TIFAGVVAPSSLALIGFLMFGNLIRECGVLNKLSLSAQNELASLVTILLGISIASTMT
                     ADRFVRLDTLMIIGLGLIAFIFDTFGGVMFAKLLNIFLPEGKKINPMVGACGISAFPM
                     SARVIHGMGQKEDPTNYLLMHAVSSNVGGQIGSVVAGGLILTLVPLFF"
     gene            22821..>23080
                     /locus_tag="PKI35_07235"
     CDS             22821..>23080
                     /locus_tag="PKI35_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNX44213.1"
                     /translation="MLIIGIAGGSGCGKTTVVKEIIRRLPKNSVTVIPQDAYYWDNGH
                     LSPEEKKEINFDHPNSIEWSLLVKHLDMLRKGETVEMPRYSYV"
     assembly_gap    23081..23180
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23390..23821
                     /locus_tag="PKI35_07240"
     CDS             23390..23821
                     /locus_tag="PKI35_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNX44214.1"
                     /translation="MLPVIRRKSIFPSIVDEFFGKDLVPGWFDSDTGITEPSVNIVEG
                     KTDFRIEVAAPGLEKDDFRITLQNNVLTISSEKETSKEEKDEKFMRREFSYAQFRRSF
                     SLPDTADSANISANHKNGVLYIAIPKKDEAKEKPAREISVS"
     gene            complement(23884..24321)
                     /locus_tag="PKI35_07245"
     CDS             complement(23884..24321)
                     /locus_tag="PKI35_07245"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNX44215.1"
                     /translation="MTKEFKFEMAHALKGYDGPCRNIHGHSYELIITVKGSPVTDESS
                     PKLGMVMDFGELKKIVRSSIIDEFDHALVINRASAGELQLLNSEIFGNAILVDYQPTS
                     ENFLIDFAGRIKPLLPAGISLHSMRLRETVTSYAEWFAEDNEE"
BASE COUNT         6642 a         5889 c         5364 g         6346 t
ORIGIN      
        1 acgcgggcgg ggaattttcc cggagaagga aatgacctgt tccctggcct gccatgaatg
       61 atcaatcgcg tattttgaaa cgatatccaa cggaaaggca gaaagcataa tacttctccc
      121 ttgatctgtt aaaaacaagc gggtagtcgt attttaccga gacctgtaag ccctgcgtcc
      181 cgaaaagcgg aatggaaacg ccgagcgctg catataaccc tttattcatc ctgtcactaa
      241 acttcagcga acccagattt tcaatatgtt tgaccgtagt atcgtttttg tcgttaaagt
      301 cattaatatc cacaattttt gaacggatga gataacttac atatccgccg ccttcaaagt
      361 acagttcttt atacagttta acccgcaaca aaaccgggac cccaacatac gttttcgtta
      421 aaaagcttcg caattcacca agcggggttc caaattgatc acttaatggc attacgaaat
      481 aattaccctt ttgatccagc ataagcccgg acctcgcgca taatgcgtcc gttaaccggt
      541 agtcggccgt aacgcctgtc ataccaccga tttttattcc gtcatcgtga ataatattat
      601 catttctgta tccggaaaat acgattccgg cttcaggagt aacagaaaca atctggctga
      661 agaggtttgt ggagccgata caaattaaaa ggatcaccaa atattttttc atcgtaaata
      721 attattaatc gattgtttaa ttgattggta ttaccgcttg ttgtgcaagt ctgatcccgg
      781 cgttcggatt gaactccatg tttgctgttt tacattcgga gacaccgcat cgctaagctc
      841 accggcgtta gtgtttatag caaatatgtt tgtttcataa ccaaaacaca tcacaaagct
      901 aataattcat aaaattatga caaaaacctg cttatcaaac actgttgtcc ggtataatca
      961 ggagttttta aatcattcac tttcagccaa aaaacattcc tttgtaaata ttccaggatt
     1021 ttgtaatttt gcgcccctta tttaaagcgt tcatttcgtg aatcctttga aagaatttat
     1081 catccccttt gtgggcctgg agcttggaaa tcacgagttt gatttcgaag ccggcgacaa
     1141 gttctttgaa caatttgaat acacgcagat tcattcggca aggctgaacg taacggttac
     1201 gctggaaaaa caggaacgga tgctcgtttt taacacccgg ataaagggcg acatcggaac
     1261 ggtatgcgat cgttgcggcg gcgacttccg tctgccggtc gaaggcagtg aacggctgat
     1321 cgtaaaattc ggcgaacagt atgccgaaga aaacgatgaa gtggtgatca tcccggattc
     1381 ggaatataaa ttcgacctgg cgccctatat ctatgaatac gcccacctca tgctgccggc
     1441 ccgcattgtt caccccgatg atgccgacgg caacccggca tgcgacccgg agatgctaaa
     1501 acgcctcagc gacctggcgc cgcacgaatc ggccgatccc cggtgggaag ccttaaagaa
     1561 tctgcatccc gaaaaagaat aaaaccagcg attttaaatc gttgtataaa agcgaacaat
     1621 aactattaat aaatacaatc atgccaaacc caaaacatcg tttttcgaaa acgagaacgg
     1681 ctaaacgcag gactcattac aaaattgatg ctcctactgt tgctatttgt tccaattgcg
     1741 gcgcttcggt gttgtaccac cgtgtatgcc ccgaatgcgg ttattacaga ggccgtctgg
     1801 ccattgaaaa aagcgccacg gcatagtggt attctccgtt ttcataggcg cctgtactaa
     1861 caaaccatat tcgacatgaa gatcggattg gatgtgatgg gaggcgattt cgcacccggc
     1921 gccgtcatcg gcggtgcgat acttgccctg aaggaactgc cttccagcga ccggatagta
     1981 ctcatcggcc ccggtgaagt gatcagcgcc catttatcgg acgccggcat ttcgccggat
     2041 ctgtttgata ttgttcatgc ccccgacgtg atcggcatga gcgaacaccc taccaaagcc
     2101 ctcagccgca agccggcttc caccatcggc gttggcttca acatgctgaa acataaacaa
     2161 ctggatgcat tcgcaggcgc cgggaacagc ggcgccatgc tggttggttc catcttcagc
     2221 gtcaactcca tccaggggat tatccgtccg gctacgccgg cttttgtccc tcaggaaaac
     2281 ggcggcatca gcgtgctgat tgatgtaggg accaatcccg atgcccgtcc cgatgctatg
     2341 cagcaattcg ccctcctggg ttccctgttt gccagccatg tgctgaaaat tgaaaatccc
     2401 cgggtcggct tgttaaatat cggggaagaa gaggaaaaag ggaaccttac agcccaggct
     2461 gtgtaccacc tgctgaaagg atgggaagaa attaatttta tcgggaacgt agaaggacgt
     2521 gatctcctgc gcaacaaagc cgatgttgtt gtatgtgatg gctttaccgg taacatcgtc
     2581 ctgaaatcca ttgaaagcat gtaccgcatc atggcgaaaa ggaaacttct cgacgactac
     2641 ttctccaggt ttaattacga gaactacggc ggaactccga tcctgggcat taacggaatc
     2701 gtgatcatgg gacatggcat ctcgaatgat attgccatta aaaacatgat cctgatgggg
     2761 cgcgatattt atgaatcccg cctcgtagca aaaataaaac actccctggt tgatattgcc
     2821 gggaagacaa atatggcgtc agccttataa actcctgaaa aacttgcaat taaaacaccg
     2881 tcggttgaca aatcctgtga atatacattc gttgcataac caggttttaa cgaaacggta
     2941 gcctgtactt aaaccagaac aaatcaggaa ttgatgacaa aaatcagagc tgcaattacc
     3001 ggtgtcgccg gatatcttcc cgaatacatc ctcaacaatg aagaattgag ccggatggtt
     3061 gataccaccg aggagtggat tatgacccgc gtcggcatca gggaacgtca tatcctcaaa
     3121 gagccgggaa aaggttcgag cgacatggga gccgaagccg ttaaaaagct cctggccaaa
     3181 accaatactt cgcccgatga tattgacctc ctgatttgtc cgacggtgac tccggatcat
     3241 cttttcccgg caaccgccaa tatcatcagc cataaaacgg ggatcaaaaa cgcctttagt
     3301 tttgatataa acgccgcctg ttgcgggttt atgtatgccc ttcaaacggc atcccaatat
     3361 atcgaaaccg ggtaccgaaa aaagatcatc gtggtaggcg ccgaaaaaat gtcggccatt
     3421 accgattata ccgaccgtac tacctgcccg ctgttcggcg atgccgccgc agccgtttta
     3481 ctagaaccga ccgaagatga agtggggatc atggatgcca tacttcacag cgacggcgtg
     3541 ggattcaccc accttcacat gaaagccggc ggatcggtaa aaccgccttc acacgaaacg
     3601 gttgatgccc gcgaacatta catctaccag gaaggccagg ctgttttcaa atgggctgtt
     3661 tcgaaaatgg ccgatgtatc ggttgaaatg atgcacaagc acaatctcac tcccgataac
     3721 ctggcatggc tcgtccctca ccaggctaac ctccgtatca ttgatgccac cggtaaccgg
     3781 atgggattgc ctcccgaaaa ggtgatggta aatatccaga aatacggaaa cactacctcg
     3841 gcaaccattc ccttatgcct ttgggaactg gaaaacaagc tgcacaaagg cgataatctc
     3901 atcctcgctg cttttggcgg cggtttcacc tggggatcca tctggctcaa atgggcttac
     3961 gatcccaaat aatacctgtt gctacatatc cggctaatta atcaaaatca tgccggacag
     4021 gtatttgcct gccgcaacag gcgcacaaat acaatattta tactcacttg cattgactct
     4081 gtaaaccccg gcaacaaccc cggtttacag gttccggaat gacccgcaca tgcaccctgc
     4141 catttgtagg aacaaacttt gctattatga aactgttaag aaacatttta ctgaaacgcc
     4201 tttcccttgc ggttggcata attctgttcc tgagtgttac agccgctgcc cagcacccga
     4261 aaaataccgc atacgttgaa aaatacaaca gcatggcagt tgccctcatg catgaatacc
     4321 agattcctgc cagcgtcata ctgggagtag ccatggtcga gagcggttcc ggcacctcgt
     4381 tattaagccg caaattccat aatcatttcg gggttatggg caaaaacacc catgccgtga
     4441 aaaaactcgg caaacattca aaatacaggg aatttgaatc ggacactgct tccttccgtt
     4501 atttttgcga actgatgagc cggaaacaat tctatcgcga actggtaaaa acttatgacc
     4561 acaagcagtg ggtgaaagcc atcagaaaat caggatatgc ctcagcagcg agccattggc
     4621 aggaacatgt tctcactgct attgaaaaaa acaaccttac ctgttacgat acccctgaac
     4681 ctgatccgct gaattatttg ccacgcgaca taaacggtgt aaaaaccaac ggtatgcttg
     4741 taaactcatg cttcgtaccg gtaaagaaaa ccgtttcgcc aattcctgcc tccaccagca
     4801 atttgaaggc ttcaaaataa gcgccgtcaa tttcgccggg cagatgcgcg tcggaattca
     4861 ccattaccgg tatattcatt gttttcaact gcttcaggat cccgaggccg ggataggtag
     4921 tatcggaacg cttcttataa atgccgcggg tgttcacttc ggcaattacc cctttctccc
     4981 tgaccagcag caacgtttcg ttcaccaggt ttttgtacca ggtttcatct tcgctgaaat
     5041 agcgcccgcg gttgtgcatt ttgatcttat ccagatggcc gatgatatca aaccgctgtg
     5101 tttccagcat ctggtttacc tgctgataat aggcagtaac agctttgcgg atatcgccat
     5161 ggaaatattc ctttaacccg gcatcgtaaa tctcggccgc cggaccatcg atgaaccaca
     5221 aatttctgcc cgaagggctg tttaccagat ggacagagcc tatcaggtag tcgggattgt
     5281 attccttttc gatggccgaa aaatcttgcg acatgccgcg cacaaaatcc acctcaaaac
     5341 cggtataaac ggcaagcctg cccgaatatt cccgcttcag tgaatccatc aaaagcaggt
     5401 attcttcttt ccgttcgggt ttaaaggaaa aaccgttttc gaatggaagc ggtgaatgct
     5461 cggtaaagcc gagtatgccg aaaccttttt caacggccaa ttcagcgtat tcgcggggat
     5521 gagccttccc gtcggaaaaa aaactatgcg tatgatggtt ggtcagcatt cgttatcaat
     5581 atagaatgaa tgtcacactc acgcccgatt tgatcgtgat gtttttaaaa gcaggttccg
     5641 gctgattttg gtcagaacgg actctgctct caaataacac tgaattgacg gctgattgaa
     5701 caaaagcgtc ggcggcagta ttttccgtta tatagatggc cttaccgatt tgctgcccga
     5761 tgcattcagc catagcttca gctttccgct ttgccgcttt taccgcttcg agtcgcaggt
     5821 cgaaacgttt ttgtgccatc tgggaatggt cgattttggt gatggaaata tttgaaattt
     5881 cctcctcact taaagccttt attactttcc ccgctgtagc ggcatcatga accagcaaac
     5941 tgaagtgttt tgagcctgca accttatctt tcaggaaata ctgcatataa ctgctcgaca
     6001 aatcttcaac ggttaaatcc cctgatacat tgattcccag ggccgtcaag cgccctttca
     6061 tcctgctttc aagcatttca agcgaaattc ttccttttgt atccgactcg tttaaaacta
     6121 tggccaggta aatcatatca ggtacaacct ccagttcggc tgtgccggta acttcaatat
     6181 atggctgatc aaggaagctc ttttgaacgg tttgggccgt cacgccgata ccggcaatgg
     6241 ccaggaggag gagaatcaat gcttttttca tgataaaaga gattggattg gttatctgga
     6301 ttttgaaaat tggacattac ttgttttctg cctccacaac ccggtacacc ttatcgttcc
     6361 tccttaccgt gcgcgaaacc ctgaaagtag gattgtcgcc tttaacaacc ttttcaacgg
     6421 ttttgtcatc attgaccatt gtatcgatca ccgcctcgta aacgccggtt gtgggcccga
     6481 caatctgcac ccggtcgccg gtattgagtt cctgcgtatc catgcgaatg tgcgcgatgc
     6541 cgcttttagc aaaatagttg gcgacgatac cgatatatat cttcttttgt gtagccagtg
     6601 aaccatgaac cggcgaccat tctccgagtt tccgtcccag gtagtaacct tcccagaaac
     6661 ccctgttgta aacggttgcc agctctttgt cccagcgagc tatattttcc ggcgtatagt
     6721 tacccgagta ataagtgtca acagcttcac ggtaacagcg ggtcaccgtt ttcacatatt
     6781 cgggcgaacg cccccggcct tcgattttca gcactttcac accggcagcc agcaccctgt
     6841 cgagtaaatg aatggttttg aggtcctttg gcgacatgat atattcattg tcgatctcaa
     6901 gctcggtttc actctccctg tcgtgaacaa tataacggcg gcggcaatcc tgcaggcacg
     6961 agccacggtt ggccgacgaa ttgtggttat gcaggctcag gtaacacttt ccgctgacag
     7021 ccatacaaag tgcgccatga acgaatattt caattttaac cctttcgcct ttgggcccgc
     7081 ggatatcctg ttcttcaatc tgcctggtga tggcggccac ctggtccagc gtcagctccc
     7141 gggcggtgac catcacatcg gcaaagcggg agtaaaattt caccgcttct atgttagtga
     7201 tgttgcattg tgtggaaata tgaatttcaa cgccctgctg ccgcgcatat ccgagtacgg
     7261 caagatcgga agcgatgatg gccgtaattt tatgctcttt cgctgcatcg acgatcgctt
     7321 tcatctcctc aatttcttca tcatatacaa tggtgttgag tgtaagatag ctcttcacct
     7381 tgtactggcg gcagatgcgg gttatttttg acacgtcgcc gagggtgaaa ttcacactgg
     7441 agcgcgcccg catattcagc ttccctaccc cgaaatatac cgaaccggcc ccgccctgta
     7501 tggcagccat cagcgattcg taggaaccga ccggcgacat gatctcaata tcgtgcaatg
     7561 ggtttttttt gcaaaaatag gaaaatcttc ggcggggaac cttaatcccg ctggagccgg
     7621 aggagcggac aaagaggttg ctctcaggga tgtcaattct tcgggatacg gcaggcgtgc
     7681 tttcttaaat tcgttcaaca agctttagga cagttgcagg tgatttatgg tcaggactga
     7741 aggcccaaga ttatacctgt ttttggttgg cctgcagttt cgggtgtccg gcgccggttt
     7801 actggtcaca gaaaccggaa tattatcatt atccgttccc ccaaatttct gttgcttttc
     7861 tcccggttca tcacggtcaa tccaaaattt taaaaatgcc cgtaacatca aagactttac
     7921 ctttgtcaag acaatttccg gattatgaaa cgcatcacta aaatattgct tgcagtgctt
     7981 accggcctgc tgatggcttt cggctggcct gaacacggat tcccgctatt actttttatc
     8041 ggatttgtgc ccatcttact ggttgagcat gatcaatgga aaaagaggaa agagaacagt
     8101 tggttcggca tctttggata tgcgtacctc gcttttctgg tatggaatgc actcacaaca
     8161 tactggattt ataattcaac gctggtcgga ggcatcgccg cgataatgct gaacacgctg
     8221 ttcatgacca ttgtgtttac attgtttcac atcacgcgcc gaaccatgat aggcgggaat
     8281 ggcggttacc tggcgctcat cggctactgg ctatcgtttg aatatcttca ccttcgctgg
     8341 gaccttaact ggccctggct taaccttgga catggttttg cagcctataa caaatggatt
     8401 cagtggtacg aatataccgg tgttttcgga ggttcgctct ggattctgct ggtaaatatc
     8461 ctgcttctta tgattatccg tgatgttctc gataaaaaat catccatcag gaaacaggcg
     8521 cttccgcttc tcggcgcctt attattgata gcattgcccg ttttgttatc cctcattatg
     8581 tataaccggt accgggaaaa acaggcgccg gtagatgtgg tggttgttca acccaatctt
     8641 gatccctata gtgagcaata cgaaatggct ccttcggatg tgctcgaccg tatgctcagg
     8701 ctggccatga ccaaagccga caataatacc caatttgtcg tttgtcccga aagcgccctc
     8761 caggaatatg tttttgaaga ccgttttgaa gaatcagcca gtttgaaagc cctgaggcag
     8821 ttcaatcaat catacaggaa aatagcgttc gtctccggca tatcaacccg gaaagttttc
     8881 gaaacgggtg aaccgctcag catgaccgcc agaaaattca aggatgtcga ccgttattat
     8941 gacgcttaca atacggccct ctatgtaaag cacgacggca catttcagaa atatcacaaa
     9001 tcgaagctca cccccggcgt tgagaaaatg ccttatccaa ggttattcaa gttcctcgaa
     9061 aattatgcca tcgacctcgg cggaacagtc ggcagccttg gcattgatgc tgatcagata
     9121 cctttcagcg taaacgactc tttaaagatt gcaccctgta tttgttacga atcggcttac
     9181 ggcgaattca tgagcaactt tgtccgcaac ggtgccaacc tgattttcgt catcacaaac
     9241 gacggctggt ggggcaacac ccccggccac cggcaacatt tactattttc atccgtcatg
     9301 gctattgaaa ctcgccgcag cgtggcccgt tcagccaata cgggaatatc ctgcttcatc
     9361 aaccagcgcg gcgatataat gcaacgtacc gcctattgga cacccacggc catccgccag
     9421 caaatcaatg caaacacggg acttacattt tatgttaaat acggcgatta tatcggaagg
     9481 ctcggaatac tcgttgcagc gctgatggcc ttactaactt tgacattcag cctccgtagc
     9541 cgcgccagat cacttcacgc ccgataatct cccggataca aaaaagcagt atccaaaaac
     9601 gaaattcatt atccggtaca aaggcttccc cttatccagg gaagcttttg ttttttacat
     9661 taataaaagt tgatatattc aggcgaattt tttttaattt cgctattcct attgtaacac
     9721 cggggttccc cctttgaaca cacaagaatt tctactcaca caaaaatccg gaagcatccc
     9781 tgtactaatg aagtattcac ttgaaaaacc tctttctact cccgaaattg agaatcccga
     9841 taccttatac ggatttataa cttttgcttc ccgttatttc atgtcggcaa accaatggga
     9901 tgaaaatctc gggtttgtcc ttgaccggtt gcaggcagtg acaacggccg gcaggatttg
     9961 ccttttttca gccgacagcc ttgagaaata cgatagtaaa gaggatatcc gtgtatggag
    10021 ttgttcaaaa agaaagcaac aaagccggtt ttcggctgcc cccgccctcc ttaccgaatc
    10081 gctcataaaa tggattcctg cattacgtca gaacagggtc atccgttcag acggaaattc
    10141 catcaatccg gcagcaggga aactaacgga atgcccatcg ctgatcatca ttccggtatt
    10201 ttcaggcaat gattggtggg gcgcccttac ccttgaattc agtgcagacg cataccgcca
    10261 aaaacggatg gaacacagga tcatcaggtt tttttgccag atgatgggtt cagccatcca
    10321 ccgtcaacga ttgattgatg ccataaaagt tgataagagc tatttcgaag aactattcga
    10381 aagctcgccg tttggaattg cagttctgaa tttagatggc gaactccagc atatcaaccg
    10441 ggggtttacg aacattttcg ggtatgatga aaaggatcta gccgggaaaa aactcgatga
    10501 tatacttcct gccccggagg agcgggaaaa tgcccgggaa ctgacagaac gctcgctggc
    10561 aggcgaagag gtatcgttcg aaaccatcag gtaccggaaa gacggcgccc ccctgtttgt
    10621 atcggttctc gtgcggcaac tgcaccccac gccttttgaa tcttctgtct atagcattta
    10681 tattgatatc acagaacgtg ttaaaaccca gcagcaactt accgaaaata aaacaaaata
    10741 tcagagcctg tttgaaacat caagcgatgg catctgcata ttcaggaaag gactcctgat
    10801 cgaagcgaac caccgtttct atgaaatgct gggatgcaga ccggatcagg tgattggttc
    10861 ttccattttc aacttttcac ctgaatttca gcccgacgga tctccttcca ttatgaaagg
    10921 caggagttat tatcgcaagg cattatccgg ggaaacagcc ttttttgaat ggcttcacca
    10981 gcgtcccgat ggcagccggt tttttggcga agtaatcctg acacgctttg agcttttcgg
    11041 acagttatat gcccagggaa ttttacgtga tatttcgagg cgcaaagcaa ccgaagaaca
    11101 cctgcaaaaa cggtacgaat tcatcaattt cctgagtaaa gcctcggccg acctgatcaa
    11161 cctggacccg gcccacatcg ataaagccat caccgaagcg ctgatgtatt cggcaaaatt
    11221 tgcaaactgc ggacggggct atgtgtatct catcgacccg gttaaaaata cttccgacct
    11281 ggcattcgaa tgggaagata gtaatatagt caatacaaat agtttgatcc ggcgccttgc
    11341 gatggaccag tatgctccgt tacaggagtt attaaatacc aggcaagtat tacaggttca
    11401 gaaatcgcaa ttgaacggac aagatgatgc gttgcatatc ggtgaacttt tcgattctct
    11461 cggcatcaaa gcgttaatca taatccctat gctggttgat aaccactaca ccgggttttt
    11521 aggctttcac agcagcgctg aaatgagctg gcccgaggaa accctgaata ctctccgcct
    11581 cagcgggcag attatcgcca atgccatcga ccggaaaagg gtgaaccggg agttgagtga
    11641 ggccaaacag aaggctgaag aatccgaccg gctgaaaaca gctttcctcg ccagcatgtc
    11701 gcatgaaatc agaacaccga tgaaccacat tatcggcttc attgagttac tgaaagaccc
    11761 ttcactatcc gaaaccgaaa gaaatgagtt tctttccatc atccggaaca gtgggaatca
    11821 cctgttaagc ctgatcgatg atatcatcga cattgccaaa atagaatcgg ggcaaatgga
    11881 attgcaccct gtcgaactat cgctcaacga tttcctgaat gatctgtacc tcgcattcag
    11941 ggaacaattc cataaatcgc cgaaaagcaa tatccatttc aggcttgacc tgccggcaac
    12001 tcccgaaaac tgttatatcc agacagatac catgagattg cggcaggtaa tcaccaacct
    12061 gctgtcgaat gccataaagt ttacgcacaa cggccatatc cgttttggat ttgaaaagca
    12121 ggacgaacac accctgcggt tttttgtgga agataccggc atcggcattc cgaaggaaaa
    12181 aacaacagac aatttttgaa agattcaggc agctcgatta cagttattcg cgcgagtacg
    12241 gcggtaccgg tttagggctt gccatttcca aagggctggt ggaattactg ggcggtgaaa
    12301 tcggggtaaa atcagagccc tctgttggat caacatttta ttttaccatt ccgtacattc
    12361 ctctgtcggc cgttcagcaa caggcggctg tatctgccaa aacttcaggg aagtataatt
    12421 ttgaaggtaa aaccattctc attgttgaag atgatgaaat caattaccac ttcctcaaca
    12481 ttatactgga acggaccgga gcgcagatac tgcatgccgc cgacggacaa aaagcaattg
    12541 atatggttaa agcaaaccat gttgacctta tcctgatgga catccgcatt cccgtgattg
    12601 acggctatac ggcgacgcgg atggtgaaaa acctcaggcc ggacctgccc gtaattgccc
    12661 agacagccca tgcgctcgac gatgaaaaaa atttatgtat cgaagccggc gccaacgatt
    12721 atatggccaa acccataaac cgcaaacagt tgctcctgaa aatttcccgt tatctcatca
    12781 acgaagaatg actgaatttg taatagttac gcaaagttgc agattaagcg ttaattgatt
    12841 gattgcaaaa tttttactac ttttgcaata ctttttagag tcaggcaacc tatggagaaa
    12901 accaaggtat tatttgtttc gcaggacatc accccttatc ttaaagatag ccctatgggg
    12961 catatttcca ggcacttacc ccagggaata caggaacgtg gccgcgaaat acgcacattc
    13021 atgcccaaat tcggcaatat caatgaacgg cgcaatcagt tacatgaagt tatccggtta
    13081 tcgggaatga acctcatcat tgacgacaca gacaatccgc ttattattaa agttgcttct
    13141 atccagtcgg caagaatgca gatttatttt atagataatg aagactattt ccagcgaaag
    13201 tacgttttcc gggacaagaa caacgtattt tttgaagaca acgatgaacg ggcaatcttt
    13261 ttttcgcgcg gagtgctgga aaccgtaaaa aaactcggtt gggctcccga tcttgttcat
    13321 tgccacggat ggatgacttc gtttatgccg ttgtatatca aaaaagcata ccacgataat
    13381 ccgttgctca gcaattcaaa ggttgttctt tcggtgtatg atgaccagtt cgatgaaacg
    13441 ctcaacaaca acatcggtaa taaaataaaa catgaaggca ttaccgccaa agaccttaaa
    13501 cattttaaaa agccgacttt tgccaacatt atgaaagccg gtatcgactt tgccgatggc
    13561 attattgtcg ccagcccgga aataaatgcc gaagttcatg aatatgcatt gaattcaggg
    13621 aaacctgtgc tggattacca gcccgaagaa acatacattg atgcatacaa cgaattttat
    13681 gatcggattc tgtcggcctg agaaaacggg aataattaaa aaaatctaat tacctcaaac
    13741 atcatatcct tgaaactgtt tcgtaacgct ttcctgtacc tgatccttgc ctcaccggca
    13801 gtgcttttta ctacctgcac caaggatccc gacttagtag gccttaacct cgtctcacca
    13861 tctgaacttc tcaaactcgg atataccgac acggctcaga ttgtcgctta tacggtaaaa
    13921 gttgatacgg ttagcaccca aaagttaacc tatgccctta tcggcagcat gaacgatccg
    13981 gtttttggtc agactgatgc aactttctat tcccagatcc tgctcgttga tgaaggttcc
    14041 gattacggta cttcccctgt attcgattca gcgtatctcg tgctgcctta tcatggcgta
    14101 tacggcgaca ccatgtcaaa catgactttc cgtatttatg aactcacgca gccattggat
    14161 gctaccgata caatgtattc cttcaatacg gttccttatg acgaatctca tttgttaggt
    14221 tccctgacat ttgtcccccg gccacacgac agcgtatata tcgacggaac aaaaactgcg
    14281 ccttcattga ggataccgct aaccgaatat ttcggccaac ggatgctttc aatttctgcc
    14341 gattcgctgg atgataatga tagcttcctg aattgctttt atggtatctg tatcgtcgcc
    14401 gaaaagcagg aaacccccgg aaagggatca atcctttatt tcacggttcc gtcgagtaaa
    14461 agtatgatta ccctgaatta tcacaataca gaagatacta caaaagccta ttatataatc
    14521 aacacgaact gtatgcgttt cgggaattat tcccacaatg gttattcctc ggcttccccc
    14581 ctgttgcagc aacaattttc gggcgatacc accgtcggaa atcaatacct cttcctccag
    14641 agtatgggag gctgcagggt acggcttcgt tttccttcgg taccggactg gcaaagcaag
    14701 caccacctca tagtcaacga tgctcaactg attttcacca atgcatcacc ggggtcacag
    14761 tataccgaac cggcttccct ggctctcatg cagattgacg acgacaacgt aagcattgga
    14821 gattatctgg acgaccaact ggaaagctca acctatttcg atggaaatta caatgaaacg
    14881 gatcatacat atcgtttcag ggttacccgg tatatacaac aattgatgac cggcaaagtt
    14941 gaaaacaacg gattgtacct cattatatca ggcgctgcca tcaattccga ccgcctgatt
    15001 cttaacggcc ctcagaaccc ttacggaaag ctgaaacttt ttgttaagta tacggtgcta
    15061 gattaataat ccctgccgtc aggccgcctt ttatccttta ggaaaaaaag agcatccgtt
    15121 ccggaaccgg aagtcctgga ccaggaaacc ggagtggaat ccttcagcct tccgtacaag
    15181 ctgggaaacc actaaaagcc tggcaaacaa cctgtacacc tgatacccgt ttaccaaaat
    15241 ccgccatcag caggaagcac aaatgctcac ctccggtatt ctttaagcac attcccttat
    15301 ttttcagcac ctgattaaaa gtttttctaa ttttactgcc ggctccatca ctttgtgacg
    15361 gtaagttaat taatcattga ttaaagtcta gtttatatca tgtgtggaat tgtcgggtat
    15421 acaggctacc ggaaagccag tgaagttctt attccaggtc tgaaacgact tgaataccgg
    15481 ggttatgaca gtgccggaat agccttgctg aacggggaat tatcagtttt taaaactgcc
    15541 ggaaaagtgg cagatcttga atcattgatc agttcctgcg acctcgaagg ttcgattggc
    15601 attgcccata ccaggtgggc tacccacggg gaacctaccg atgccaatgc gcatccccat
    15661 ttgtcaaaat caggcaaaat agccatcatt cacaacggca tcatcgaaaa ctatgccgta
    15721 ctgaaggaag agttgatgaa ccggggctat actttcaaca gtgaaaccga taccgaggtc
    15781 ctggcacacc ttattgaaga tgtccagtta aatgaaaaat gcgggctggt tgaagccgtc
    15841 cgtctggcgc tggggcaggt aaccggggct tatgcgattg ccgtgcttgc cgaagaggag
    15901 cctgataccc tgattgctgc ccgaaaaggg agcccactgg tgttgggtat cggcgaaggc
    15961 gaatatttcc tggcttccga tgcagccgcc attgtagaat atacacgaca ggtaatctat
    16021 ctgaacgatt acgaaatagc catcataagc cgggaaaacg gctaccggat catcacggta
    16081 tccaataccg aaaagactcc ttacatacaa acccttgaag taagtctcag cgcacttgaa
    16141 aaaggcggtt ttgagcactt catgctgaaa gaaatctttg aacagccgcg aactgtccgc
    16201 gacagtatgc ggggaaggct gaatatggaa caaggatcgg tgatccttgg cggaattgaa
    16261 gaatacaccc gtaaactgtt aaatgccagg cgtatcatca tgctgggctg cgggacttcc
    16321 tggcacgccg cccttgtagg agaatacctg atcgaagaac tggcccgcat tcccgttgaa
    16381 gtggaatatg cttcggaatt ccgttaccgc aacccgctga tcagtgaaga tgatctggtc
    16441 attgccattt cgcaatcggg cgaaaccgcc gataccctgg ctgccatcga acttgccaaa
    16501 tcgaaaggag ccaccatatt gggcatttgc aacgtcgttg gttcctccat tgcccgggca
    16561 acccatgcag gttcctatac ccatgccggt cctgaaatcg gggtggcttc aaccaaagcg
    16621 ttcacctcac aggttaccat attgaccctc atggcgctga tgctggccga acgtaaaggc
    16681 accatcgcag catcgcgtta ctatcgcctt ttacatgaat tgaactcggt tcccgaaaaa
    16741 attgagaaag tgctgcagtc caattccagg atcagggaaa ttgcggtaaa gttcagtaaa
    16801 aagcgaaacg ccctttacct cggacgaggc attaattttc ctgtagccct cgaaggcgcg
    16861 ttaaaactga aagagatctc ctatatacac gccgaaggat atccggcagc cgaaatgaaa
    16921 cacggcccca tcgccctgat cgacgacgaa atgccggtgg tagtcatagc taccaggaaa
    16981 ggtaattatg ataaagtgat cagcaacata caggaagtga aagcaagaaa aggaaaggtg
    17041 ctggctattg tttccgaggg cgatgaaacc gtttgctcaa tggccgatca ttgcatcgca
    17101 gttcccgaaa ccgatgagat gctcgttccc cttattgcga ctgttccgtt gcagttgctg
    17161 gcttatcatg ttgccgtgtt gcgtggctgt aatattgacc agccaaggaa tttagccaag
    17221 tcggtaacag tggagtaagg caccatgcag aaggatgctt caggtcagca gcattgaccc
    17281 tgaaaggatc ctgtttcctg ttggtatcgg ctttattatc ttttgctttc agactctatc
    17341 aggcattagc cctcatgtta tgcctgttct gttaaaaagg gcctcatcca gggcatttat
    17401 ccatttggta tatgtaaaat attttgaata tatcataccg gtattttcaa aggtcaaaaa
    17461 gtcctgtttt tcaagataat ttattccgtt tctgtttatt gaataacaaa aacaaatatt
    17521 tttgcaccaa aattaggggc tatgaatatt tcgcacatcg agcacatcgg aattgctgtt
    17581 aaaaatctgg aagaaagtat tttatactgg gaaaaagtat ttgggttaaa atgctattcc
    17641 atcgaagagg taaaggatca aaaggtgaag accgccttct tcatggttgg gcagaccaag
    17701 atagagttgc tcgaaagcac cgatccggaa ggcccgatcg gaaaattcat cgagaagaaa
    17761 ggggaaggca tccaccacat ggcctttgcc gtcaaagaca ttgaaaatgc tttaacggaa
    17821 accgaagcta aagggattca actcattgat aaaacgccgc gccaaggcgc cgaaggcctt
    17881 cagataggtt tccttcatcc aaaatcaacc tttggcgttc tcaccgaatt gtgcgaggat
    17941 aaaaacaaat aatcctttct tactgaattc ttcattccgg agcacaagat caatccttaa
    18001 aggatcataa atcatttata ataatacact acatcatcat ggcaatagaa aataaaattc
    18061 aacatctcct cgataaacgc gatgaagctg cattgggtgg agggaaaaag cgcattgatg
    18121 cccagcataa aaagggcaaa ttaacggccc gcgaacgcat tgatttactg ctggacgaag
    18181 gcagttttga agagtttgac atgttcgtaa cacaccggtg caaagatttc ggtattgaaa
    18241 atgaaaagta tcttaccgat ggcgtgataa ccggttacgg aaccatcgac ggcaggatag
    18301 tttatgtatt ctcgcaggac tttaccgttt tcggaggttc gctgagcgag atgtttgcca
    18361 ggaagatttg caaggttatg gatcaggcca tgaaaatggg agccccgctg atcggaatta
    18421 acgactcagg tggcgcccgt atccaggaag gcgttcaaag tctggcaggg tatgccgaaa
    18481 ttttcgaacg caacatcctt gcttccggcg ttattccgca gttatcagcc atcttcgggc
    18541 catgcgccgg cggagctgtt tacagcccgg ccctcaccga ctttatcgtt atgtcgaaaa
    18601 caaacagcta tatgtttgtt accgggccca aagtggtgaa aacggttatc ggcgaagtgg
    18661 taaatgaaga tgaactcggc ggcgccatgg tgcacggagc aaaatcggga gtatcccatt
    18721 ttgttgccga agacgagcag gaaggcatca tgctgatccg gaaactttta agctatttac
    18781 cccagaacaa cctcgaagaa ccgccgttgc ttccgtgtga tgacccgatt gaccggcttg
    18841 atgattcgtt gaattacatt atccccgaca atcccaacaa gccttacgat gtaaaggata
    18901 tcatccattc catcgttgat aatggcgaat ttcttgaaat agcaaggcat tttgcaccca
    18961 atatcgtcat cggctatgcc cgcatgaacg gcatgtcggt aggaattgtg gccaaccagc
    19021 caaacttcct ggcaggcgtt ctcgatatta acgcttcacg caaagccgcc cgctttgttc
    19081 gcttctgcga tgctttcaat atccctatcc ttacgctggt cgatgttccg ggattccttc
    19141 cgggaaccgc acaggaatac ggcggtatca ttatccacgg ggcaaaactg ctctatgctt
    19201 acggcgaagc aaccgtgccg aaagtaaccg ttaccctgag aaaatcttat ggcggcgctc
    19261 atgatgtgat gagttcaaaa caactgcgcg gcgatatcaa ctatgcctgg ccctcggccg
    19321 aaatagcagt gatgggacct tcaggagcta tcgaagtcct ttataacaag aaaatatccg
    19381 ccgttgaaaa tcaggcagac cgtgaagcaa tgatcagcaa atacgaaaac gaatacaagg
    19441 acaaatttgc caatccttat gatgctgctt catatggttt tatcgacgat gtaattgttc
    19501 cgcgcactac ccgtttcagg gttatcaggg cattccaggc gctggctaca aaaaaagacg
    19561 taaatcctgc caaaaagcac ggcaatcttc ctttataaac agtaccacca tgagcatatt
    19621 caagaaaaag gatcaaaaca acggagccga acaacccgta aaaccggaag ctgttcagtt
    19681 gccggagaaa aaagaggtgg agcctgaaat agctgctgtt atcgcattgg ctatgcatct
    19741 ttacgacatg caaatgcggg aggatgaaaa tgcgatcatt accattcaga gaataatgaa
    19801 accttattct ccctggaatt caaaaatcta cggcttgcgt cagcaacccc tccgcatccc
    19861 gggattaaga acaaggatca aataattttt aaacagaatt tgaacaatga aaaatctgaa
    19921 attcaagata aaggacactg aatataatgt tgatattttg agtaccgact taaaccaggc
    19981 caaagtcagt gtcaatggcg aggtatatga tgttgagatg gaagcgccta ttaaagccac
    20041 caaaacgcct accctggtcc gtacactggc tgttcccgat acaaacacca ccccttcaac
    20101 tgcccgcact gccaaacctt cggcacctaa aggcaccggc gctatgaaat cgccgttgcc
    20161 cggtaaaatt ctcgacatct ttgtcaaaat aggtgattcc gttaaagtag gccagaaagt
    20221 gctctgcctc gaagccatga aaatggaaaa caacatcaac tctacccgcg atggttcagt
    20281 cactgctata cttgtacaaa agggtgattc cgtcctggaa ggtgatgttt tagtcgaaat
    20341 caattaatac accctactaa cttactaaaa tgaactcttt aaccattttt tgggtagcct
    20401 tcattgtact ctttttcctc gttttcggta ttgatttgta cgtaacagac cacagaaaag
    20461 gggaattaag cgtaaaaaca tcgctacgct ggacggccgt ctggatttca attgctttat
    20521 tgttcggagt ggccatttat ttcttcttcc ctcaaaatcc ggaaagcacc gtcaggacat
    20581 cctcaaccat gatgtttaaa ttcatctcgg gatacctgac agaatactcc ctgtcggtcg
    20641 ataacctttt tgtgtttatc atgattttct cgctgatggc cgtccatccg aaaaatcagc
    20701 ccaagttgct caaactcggc atcctgctct cgattgtgtt acgcatcctg ttcattgttg
    20761 ccggaatgga actcgtacag aggtttcatt ggatcattta cattttcgga gtcatcctgt
    20821 tatggacggc ttacaaaatg gccttcacat ccggcgatga ccaggttgat ccgaaaggaa
    20881 acatcctgta ccgtactgcc tccaaaattt ttcccctgga tccggatccg gatgcaaacc
    20941 atttcatgcg caagatgaac ggaaagtggc atatcaccat gatgttcctt gttttcctgg
    21001 taatcggttc aacggatgtg ctttttgccg ttgactcaat ccctgccatc atcggagtaa
    21061 ttaaagaagg cgcttctcaa atcctcaccc ctcaggaaga agattttatc gccatcactt
    21121 cgaatgtgtt tgccgtgatg ggcctcattt cactgttctt tgccttaaag ggcatcatgg
    21181 gcatgttccg tttcctgaaa catggcgtaa gttttatcct gtttttcatc ggcgctaaga
    21241 tgttgctgac ctcggtacct gctgtggaac atttctttgc tgcacactcc tgggtatcgc
    21301 tggtcgtcat catttccacc ctggcaattt caatcctgtt atcgatggta attgctgaaa
    21361 aagaggaaat agaagacctt catgaagatc ttgatgaaat acaacacgca ctggaagata
    21421 aagataaaaa cgtttaacta tggatattaa agaactgctg cccgtactat acagtatcac
    21481 gtggcaaaat ctggtgatga tcgcggtagg tttgaccctg atttacctgg ccattgccaa
    21541 ggaatatgaa cctaccttat tgctccccat gggatttgga gctatcctgg taaatatacc
    21601 gctgacatcc gctattacgc agattgatcc ggtgaccggt caggccatcg aaggcgcatt
    21661 gtcggtattc taccaggcgg gtattgccac tgaggtcttc ccgctgctca tctttatagc
    21721 gatcggggct atgatcgatt tttcgccttt gtttaaaaat ccgttcctgc tcctttttgg
    21781 agctgcagcc cagtttggta ttttcttcac catgatcgcc gctactctgt taggcttcga
    21841 tctgaaagaa gctgcttcca ttggtattat cggcgccgcc gacggtccca cctccatctt
    21901 tgtttcttcc cgctttgcac cgaaccttct cggtcctatt tcggtagccg cctattctta
    21961 tatggcattg gtgcctatca tccagcctcc ggtaatcagg gcgcttacct cgcgtaaaga
    22021 aagaatgatc cacatggaag gacatgctaa aagtgtttca aagagcgctt taatagcttt
    22081 cccgatcata gtcaccattt ttgcgggggt tgtagctcct tcctcgctgg cgctgattgg
    22141 tttcctcatg ttcggcaacc tgatccgcga atgcggcgtt ttgaacaaac tctcactgtc
    22201 ggcacagaat gagctggcaa gcctcgtaac catcctcctc ggcatcagta tcgccagcac
    22261 catgacggcc gatcgttttg taaggctcga caccctcatg atcataggcc tcggtcttat
    22321 cgccttcatc tttgatactt ttggcggtgt aatgtttgcc aaactcctga atatcttttt
    22381 accggaaggc aaaaagatca atcctatggt cggcgcatgc ggaatttctg cattccctat
    22441 gtcggcacgt gtaattcacg gaatgggcca aaaggaagat cctaccaatt atctgctcat
    22501 gcacgccgtt agttctaacg tcggcggaca gataggctct gttgttgccg gaggccttat
    22561 tttaacgctg gttcctttat tcttttaatt aaaaggaggt ataagatgaa tgctttaagt
    22621 gctgcacttg aaataacggt tttcggaatg ctgggtattt ttgcctttat gattattttc
    22681 tacttttcaa tcaaggcaat tgacaaaatc tggccctata aaaaggaatc cgataattaa
    22741 tacgtaatca acaaaattta gattgcatca gatttataat ctggtgcaat cttttatttt
    22801 tgccttataa atatatctgt atgctcatta tcggaattgc cggaggatcc ggatgcggaa
    22861 aaactactgt cgttaaagaa attatcaggc gtttgcccaa aaactccgtg actgttattc
    22921 cacaggacgc ctactactgg gacaacggcc acctctcgcc ggaagaaaaa aaggaaatca
    22981 acttcgatca tcccaactcc atcgaatgga gtctgcttgt gaaacacctg gatatgctca
    23041 ggaaaggcga aactgtcgaa atgccccgat attcctatgt nnnnnnnnnn nnnnnnnnnn
    23101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23161 nnnnnnnnnn nnnnnnnnnn tttctcctgg tcctttccca tgcctctccc cgattgacaa
    23221 gcgctgacaa tcggacagac aaagacggaa aataagacaa tttttcttaa taatatttgt
    23281 ataactgcca tttttacagc gtattgggta tttattccgg ttggtacgga gattgttcga
    23341 caacagtcaa aaccttaata ctaacaatta aaaaatggag gattaagcca tgttaccagt
    23401 tatcagacgt aaatcaattt tcccgtcgat cgtggatgaa ttttttggga aagatctggt
    23461 tccgggctgg ttcgacagcg ataccggaat caccgaaccc tcggtaaaca ttgttgaagg
    23521 aaagaccgat tttcgtatag aagtagcggc accaggcctt gaaaaggatg atttccgcat
    23581 cacgcttcag aacaatgtgc tcaccatctc atcggagaaa gagacttcca aagaggaaaa
    23641 ggatgagaaa tttatgcgcc gtgaattcag ctatgcacaa ttcagacgtt cgttcagcct
    23701 gcccgatact gccgactcgg ccaatatctc ggcaaaccac aaaaacgggg tgctctatat
    23761 cgctattccc aaaaaggatg aagccaaaga aaaacctgcc cgtgaaatat cggtttcata
    23821 aaaaccgcct tcgtgacaaa taaagggttg ccagcaatga caaccctttc tattttattg
    23881 taattattct tcattatcct ccgcaaacca ttcggcataa gaggtaacgg tttcgcgaag
    23941 tcgcatacta tggagcgaaa tacctgcagg cagcaggggt ttaattcttc cggcaaagtc
    24001 aatcagaaag ttttcgcttg tcggttgata atccaccagg atggcatttc cgaatatctc
    24061 tgaatttaaa agttgcaact ctcctgccga tgccctgtta atgacgagtg catgatcaaa
    24121 ttcgtcgatg atgcttgacc gtacaatttt tttcagttca ccgaaatcca ttaccatgcc
    24181 cagtttcggg gacgattcat ccgtgaccgg actccccttt accgtaataa tgagttcgta
    24241 tgaatgcccg tgaatattac ggcatgggcc gtcatagccc ttgagggcgt gcgccatttc
    24301 gaatttgaat tcttttgtca gcctgactgt tgtcactatc t
//
LOCUS       DAOPYV010000044        23393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_2186_length_23393_cov_11.969792, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000044 DAOPYV010000000
VERSION     DAOPYV010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23393
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2186_length_23393_cov_11.969792"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(436..2109)
                     /locus_tag="PKI35_07250"
     CDS             complement(436..2109)
                     /locus_tag="PKI35_07250"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HNX44216.1"
                     /translation="MTKYTLGVDYGTDSVRTILVDTADGTEMASSVFYYPRWKQGKYC
                     VPAKNQFRQHPLDYVEGLENTVRDVISQVPPEVAANIVAISVDTTGSTPVAVDKNGTP
                     LALLPEFADNPNAMFVLWKDHTATAEADQINTTARNWGGTDYTKYEGGIYSSEWFWAK
                     ILHVIREDERIRQAAFSWVEHCDWIPALITGTANPLTMKRSRCAAGHKAMWHKDFGGL
                     PPEEFLLKIDPLLAGLRNRLFTDTYTCDVKIGNLTPEWAGRLGLSEKVAVGAGAFDAH
                     LGAIGGEIKPYYLSKVMGTSTCDMMIAPMEEVGNKLVHGICGQVDGSVVPGMLGLEAG
                     QSGFGDIYAWFKKLLMWPVGELLAKTSVIDDPAKARLIEEISGKLIPQLTAEAEKIPV
                     GESGVLALDWMNGRRTPDANQLLKGAILGLNLGSDAPRIFRALVESTAFGAKKIVDRF
                     ISEGIPIEGIIALGGVAKKSPFIMQVVADVINKPILVARSEQACALGSAMAAAVVGGI
                     YTDVPEAQKYMGGGYETEYHPDPANAEKYKALYDDYSRYGEVIEKEIMK"
     gene            complement(2116..4167)
                     /locus_tag="PKI35_07255"
     CDS             complement(2116..4167)
                     /locus_tag="PKI35_07255"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNX44217.1"
                     /translation="MDEKTINRAADNIRILAAAMVEKAKSGHPGGAMGGADFINILFT
                     EFLRYDPSDPAWHMRDRFFLDPGHMSPMLYAVLTLSGKYTIDEIKNFRQWGSPTPGHP
                     ELDVTRGVENTSGPLGQGHTMAVGAAIAERFLCARFGEWMSHKTYAYISDGGIQEEIS
                     QAAGRIAGYLGLSNLIMFFDSNDIQLSTETRAVTCEDTAKKYEAWGWKVLTINGNDAS
                     AIRNALTEANDEKEKPVLIIGKTIMGKGALKADGTSFERQCATHGMPLGESGASFEKT
                     IEHLGGNAQDPFAVFPEVAELYEKVKAKNIALASYRKSQQQEWESEHTEQAATLHRYL
                     KGELPELHFDEIAQKPGIATRAASSAVLGYLAQKAGNMIVSSADLANSDKTDGFLKYT
                     KPFTKGDFSGAFLHAGVSELTMAAICNGIALHGGVFAACATFFVFSDYMKPAIRLSAL
                     MGLPVKFIYSHDAFRVGEDGPTHQPVEQEAQIRLLENLKNHRGHMSLLALRPADAAET
                     TMAWKMAMENNNSPTALILSRQNIADIPAKAGSTRYADAQQASKGAYIALDGGGVPDI
                     VLIASGSEVATLIESAGLLKTRNNLKVRVVSAISEGLFRQQPEEYRQQVIPCGIPAFG
                     LTAGLPVTLAGLVGAHGRVWGLDHFGYSAPAKVLDEKFGFTPENVYNQVIAFLGETKK
                     S"
     gene            complement(4185..4868)
                     /locus_tag="PKI35_07260"
     CDS             complement(4185..4868)
                     /locus_tag="PKI35_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HNX44218.1"
                     /translation="MLTELKHRVYEANLGLVKRNLVLYSWGNVSGIDRDENLIVIKPS
                     GVLYENLTPFEMVVVDLNGRVVDGNLKPSSDTLTHIELYKAFPGIGAIVHTHSTYAVA
                     WAQACKPIPVYGTTHADHFSGPVPCTRMLTQQEVESAYEKETGRVIVETFKDIDPLHV
                     PGVLVGNHGPFTWGETPEKAVYNAAVLEEVARMAWLSVQLGAAGPIPDYLLAKHFNRK
                     HGKNAYYGQ"
     gene            complement(5005..6519)
                     /gene="araA"
                     /locus_tag="PKI35_07265"
     CDS             complement(5005..6519)
                     /gene="araA"
                     /locus_tag="PKI35_07265"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HNX44219.1"
                     /translation="MKTFNNLEVWFVAGSQHLYGPEVLRQVAADSETIAAAFNNSSEI
                     PVNIVFKPVMTTPDDITNLCIDANASASCIGLITWCHTFSPSKMWINGLKKLQKPLLH
                     LHTQFNRDLPWEEIDMDFMNLNQSAHGDREHGFIMTRLRMNRKVVVGHWQDPEVVGDI
                     AVWARAAAGWHDMQHMKIARFGDNMREVAVTEGDKVAAQIQFGVSVNGYGVGDLVRFV
                     DDVPEPVVDDLVKEYTEIYNVAADGMTGGKYHENVRYQARLEAGIRAFLEQGGFSAFT
                     TTFEDLHGLKQLPGLASQRLMASGYGFGAEGDWKHAAMVRVCKTMGKGLKGGCSFMED
                     YTYHLDPAGMKVLGAHMLEICPTLSGHKPKLEVHPLGIGNKEAPARLTFSVPAGPATA
                     TSLVDMGNRFRMIVNSVDCVVPDADLPKLPVARALWIPQPDLKTGAAAWIYAGGAHHT
                     AFSQAVDARYIEDFCDMAGIEYVHIDKDTCLSSFRKELKWNDLYYMQAGALKFL"
     gene            complement(6574..8148)
                     /locus_tag="PKI35_07270"
     CDS             complement(6574..8148)
                     /locus_tag="PKI35_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008782201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNX44220.1"
                     /translation="MKSSLTTIFLAFICAVSGGTVIAQENTVTMQPEKAGTVISKNLY
                     GHFAEHLGHCIYGGIWVGENSSIPNVRGIRSDVVNALRDMSIPNLRWPGGCFADEYHW
                     KNGIGQREKRPAMINTNWGGVSEDNSFGTNEFLDLCEQLGCEPVITGNLGSGSVEEMS
                     QWVEYINSGNISPMTDLRKAGGREKAWNVKFWGLGNESWGCGGAMSPEFYFDQMMRYS
                     NFCRDYDGKGLFRIAVGPNVDDYNWTETMMRLWSKKSWWEQRLMNGLSLHYYTLCHGW
                     GDKGSATEFTEKDWFATLKATCRMEELIGKHLAIMDKYDPGHRVGLVVDEWGNWYDVE
                     PGTNPGFLFQQNTLRDALVAAINLNIFNNHADRIVMANIAQMVNVLQSVILTDKDRMA
                     LTPTYYVFRMYKVHQGATLIPVTVSCRNYASGDESVPAVNVSASKDASGRIHVTMVNL
                     DPGNETPVTVNLGSEQKVTVTGQVITSENMNDYNDFGQAPKVIAAGFDKFKAGNGQVK
                     VSMPSKSVVCLEITAR"
     gene            8459..9508
                     /locus_tag="PKI35_07275"
     CDS             8459..9508
                     /locus_tag="PKI35_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNX44221.1"
                     /translation="MKFFRLPFLIAILTFTGARYILAQETNRDCIIRNEFIFDSAPFA
                     ECHASTIAETADGLAVAYFGGTAEKNPDVAIWISLYRNGKWDVPVEIANGISPDGKRY
                     PCWNPVLYRDGSGVLLLFYKVGPDPVNWWGMIMKSADGGRTWSVPARLPDGILGPVKN
                     KPVRLQNGELLCPTSTEAGEWQVYFERTADLGLTWSKSGFVDDPRHMQAIQPAILFHG
                     RKRLQALCRSRGNVIAESWSKDNGKTWTPLAPTSLPNPNSGIDAVTLTDGRQLLVYNP
                     SNVPEGKWTGPRSPICIAISKNGKVWHKVLTLDSEPGELSYPAVIQTSDGMVHITYTW
                     KRTTIRHITVDPDNI"
     gene            complement(9654..10130)
                     /locus_tag="PKI35_07280"
     CDS             complement(9654..10130)
                     /locus_tag="PKI35_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44222.1"
                     /translation="MKIPASILVLVFLSMSVFATGVIENFVIADGVVYQITTMRKGIT
                     NTRITTTSGEIVKIPNASIKSYRINGHQYDMLPMLNQRGDTIAAAFMELIAARNGSRL
                     YRYCSNCNKYDPVNQEIAPINRVYRYYVLKSGRLNLLYDEETTGKAMAFFSVRILK"
     gene            complement(10175..10648)
                     /locus_tag="PKI35_07285"
     CDS             complement(10175..10648)
                     /locus_tag="PKI35_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44223.1"
                     /translation="MKTFLIALFMMVLALAASAGETVNYVTVKGKTYFCKELKSGMFR
                     LGIVLNDGTTLKVPFNKVDAYMCKGHLYERLPLMCPNAKPGCTALLEFVTARNGLRLY
                     KYCKYGECCDPASNSYKKAEAQYLYTVFKDGKFYLRVDQENAPTVLPFFGIEAIY"
     gene            complement(11398..12129)
                     /locus_tag="PKI35_07290"
     CDS             complement(11398..12129)
                     /locus_tag="PKI35_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX44224.1"
                     /translation="MKINCIAIDDEPLALDIIRDYCTKVPFLNLLNTFDNAIETLEYL
                     RNNHVDLILLDIQMEELTGLQLLNSLNVKPYVILTTAYDSYAMQGFELDVADYLLKPI
                     SFERFLKGVNKVYDRMRLGNGDQTEKLPTPHIPESLDKDYFFVKTETRIEKVTASEVL
                     YVEGMGDYWRIVTRNKRIMTLMNAKGIEDVLPEPQFCRVHKSYFVAVDKIEFVERKHI
                     KIADQRIPISDTYQKNFFDIIEKRK"
     gene            complement(12131..13159)
                     /locus_tag="PKI35_07295"
     CDS             complement(12131..13159)
                     /locus_tag="PKI35_07295"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNX44225.1"
                     /translation="MKKKTVIIIHLLYWFYFINQSLYPIYIGQLDKANMIDNQYLKNL
                     LITTFLNLITFYAIYFSFPRLLKLKNKVLAAGTGLVMLAMLTTFRVITDYLFWKYFGN
                     LPQQELIFKWVWVWGDVRLVIITGTYAVLISFMISWFETQKLKDELINQQQAGELALL
                     RSQVNPHFLFNTLNNIYSLVYRKSEDAPAAVMKLSSIMRYVLYDATSDKVPLEKEIEY
                     LKSFIELQKLRIKHPDFVSLSIEGNTDNRTIAPMLLIPFVENAFKHGSKNHEPGIYIR
                     LKTEQDWLRFEVINYIRKTTAAPVQQASGIGLANIRRRLDLLYPDKYQLIIKQDEEKY
                     RINLEIEN"
     gene            13472..14212
                     /locus_tag="PKI35_07300"
     CDS             13472..14212
                     /locus_tag="PKI35_07300"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNX44226.1"
                     /translation="MKKLIMISLFLVAATGLSLAQEDTAPQSIPLIGSIAPSFTAQST
                     QGTITFPEDYGRKWKILFSHPADYTPVCSSEILAMATMQQDFDKLGVKLAVVSTDALD
                     KHYNWKKSLESVRYNGHEPVKINFPLIDDQNKSIARLYGMLHPESPSSRDVRGVFIID
                     PADKVEAVFFYPNNVGRNMDEIKRTVIALQTASDYSVLTPANWKPGEDVLLPYLKSVD
                     EALVQSGKENPEFYSLTWYMWYKKLDVK"
     gene            14477..15172
                     /locus_tag="PKI35_07305"
     CDS             14477..15172
                     /locus_tag="PKI35_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX44227.1"
                     /translation="MEKAVKNAMRGLIIPVMVLIANSAAGQGKTIQIDFLGNCGLFMT
                     DGTLNICIDFPYKSGAYGYMTYNLNLPDSIPENTIYIFTHGHADHFDRKLFKETNRQL
                     FGPWPVKAFISGKRKLSFHELNAISQNFSVQKFTTRHRLSLKHYSYLIEWDGKRIYIS
                     GDAENADVIGNMKNLDLVFAPGWLLNDASDKNIAIDTKKIVLYHHVSVEGIINNSKDK
                     IIVPSRRQLIELK"
     gene            15449..16273
                     /locus_tag="PKI35_07310"
     CDS             15449..16273
                     /locus_tag="PKI35_07310"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNX44228.1"
                     /translation="MILQETYHLLETRYRKYLDNVSIEKVQAGIFLTAVQLSTGYCGV
                     ASTDLDSTVNCSSRQNRDFGDFTPGNIKGQNVTRLFQFPGDSRILDSVKLAALNAVSA
                     GIILQSDYQIIEDRDPFDLLNLDGQKRICIVGAFQDYIKKLAGSGHQLHVLELNKDAL
                     TEEQQQYYVPAADAPAVIPVADIIILTGSTLVNNTLDNLLTLIPAGKQAVVVGPSSSL
                     IPDILFKYNVSIIGATKITDPDRMFAIVAEGGLGYHLFHYCAKKICLINNLPPSER"
     gene            complement(16367..16439)
                     /locus_tag="PKI35_07315"
     tRNA            complement(16367..16439)
                     /locus_tag="PKI35_07315"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16404..16406),aa:Gly,seq:ccc)
     gene            complement(16503..17792)
                     /locus_tag="PKI35_07320"
     CDS             complement(16503..17792)
                     /locus_tag="PKI35_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNX44229.1"
                     /translation="MISTINQLPGWSRNLTIYEVNLRQYSPGGTFREFREHLPRLKKL
                     GVGILWFMPLYPIGVVNRKGSLGSYYSIRNYRAVDELYGTLEEFTGLVKEIHDLGMYV
                     ILDWVANHTAWDHVWTKDHPEYYRLDVKGKFMPPFSEWEDVIGLNYNSTGMREAMIGD
                     MCFWIENTGIDGFRCDMAHLVPTSFWEEAKEKLVTLKPDLFMLAESELRELVNKAFHV
                     LYNWNLYHCFNDMAQGKKTVTDLRMLLQQEIFNFPKHAANMLFISNHDENSWNGSEIE
                     RLGVALEAFAVLIFMLPGMPLVYSGQEAGNAKRLKFFDKDLIEWREDKMFAVYDKLIA
                     LRDGHPVFHRGADPSDFSVIPTNCDNQVIALKFSEGENQVMAILNLSPRAASFSVFTR
                     AFPGEVYELFSPPVPVALGENPGFTLGAWQYKVYYSA"
     gene            complement(17807..18445)
                     /locus_tag="PKI35_07325"
     CDS             complement(17807..18445)
                     /locus_tag="PKI35_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44230.1"
                     /translation="MLNDLVSINKKHEEAALSVYERINTERVPKYIVTLSGEVSSGKC
                     EISNALARLYRKDGVKCKILHMDDYYTIPPEKRTAWRMKHGIGSIGYDEYDWPRIRKN
                     IAEFRNGKKSSLPCVDLVTLQVDELITDFSEIDMLIINGLYSVKIQEANLRVFIELTY
                     KETLEQQIAEKKEELDEFRMKVLEREHLVVQSLKPEADFYIDFDNSLEAFHL"
     gene            18663..19457
                     /locus_tag="PKI35_07330"
     CDS             18663..19457
                     /locus_tag="PKI35_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HNX44231.1"
                     /translation="MPTNKHTRRIILRAFLIPSLLLFALWIIIFSIRTVFRKGPDQPG
                     FFQGNTAFGLTIPGKYAVFGIDVSHHQGSVNWSKVKEMVSNNRKVTFVFIKATEGITR
                     QDQNFEENWEAVRKEGFIRGAYHFFYPSRDAGRQAENFISQVSLSKGDLPPVIDIEHT
                     NGRTKKQICEGLKAFIDEIEAEYDVKPIIYTNISYYNNYLSDDFADYPLWISGYHGED
                     KFREKFKSGWHFWQYSEDGQVDGIKGEVDCNVFKGSESDLQKMCIR"
     gene            complement(19649..20764)
                     /locus_tag="PKI35_07335"
     CDS             complement(19649..20764)
                     /locus_tag="PKI35_07335"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNX44232.1"
                     /translation="MKRVMVLTGGGDCPGLNAVIRAIVKRASQESGWEVLGCIKAFDG
                     ILNEPTEVVMLDDAKVAGIHYQGGSIIQTTNKGGPFAWPVKKHDGGWEYVDRSDEMIR
                     KIQYLGIDAVINIGGDGSQRISQQLFEKGLNIIGVPKTIDNDLSATDSTFGFQTAIQE
                     ATSAVDKLVTTAASHNRVFILEVMGRDAGWIALNSAVAGGAEVCLIPEIPYDLGIVKS
                     KIDSRFHRDRGFAIVVVAEGAKSKDGKVVGHKAQEVGYENVRLGGVGFKLAEDLQRAG
                     CEHDIRVTVLGHLQRGGIPIAYDRVLATQFGVKAMELVLKGEFGRMVSYRHPYITSVT
                     LKDAIQQPNLVTLDTALIKTARGVGICFGDDPGAYEE"
     gene            21066..21410
                     /locus_tag="PKI35_07340"
     CDS             21066..21410
                     /locus_tag="PKI35_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44233.1"
                     /translation="MKIIISAFILTVLISLNVKAFTPSAFLPMAAETYVNDIPFNTAL
                     IATFTHSGRSLNSCIRIADEPFVNDIPFDTRAVAEKEKRNLPVLPYLQPADEPYADDL
                     PFNTAAIVKLLQ"
     gene            complement(21472..21867)
                     /locus_tag="PKI35_07345"
     CDS             complement(21472..21867)
                     /locus_tag="PKI35_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44234.1"
                     /translation="MSNNFLCPYCRNYLNVGENVVFSTRNRIGKEGIIMLHPKLGDYS
                     VIKHPTFRIGKGELVDFFCPFCNKKLVSDRNPNLAKVLMLDEKDTEYEIHFSRIAGQH
                     STYKIIGKAVEIFGEDAADYFDFLNTGNM"
     gene            22015..22695
                     /locus_tag="PKI35_07350"
     CDS             22015..22695
                     /locus_tag="PKI35_07350"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNX44235.1"
                     /translation="MNQKHCYALVFDMDGVIVDNHQWHFRAWLEFCRQHGFRLSEEEF
                     YTTIFGGTNRDLLTRLYGNTLTDAEILQMGEEKELLYRTVYEPHIKPVPGVREFILSA
                     RERGIRTALATSAPMANVTYTLKHTGLENNFDVIVESSQIAHGKPHPEIYIRVASLLG
                     LRTERCAVFEDSLPGIQSAREAGCKVTGVATTLQASEISHADLVISDFTDQQTLFAVI
                     DSWFVNRM"
     gene            22891..>23393
                     /locus_tag="PKI35_07355"
     CDS             22891..>23393
                     /locus_tag="PKI35_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNX44236.1"
                     /translation="MRIKLLALLSVFLITGLTYAQQGNIKFTEYDMDNGLHVILHQDN
                     TTPIVAVTILYHVGSKNEVEGRTGFAHFFEHLMFEGSDNIPRGEYFKITQAAGGTLNA
                     NTSEDRTFFFELLPSNQLELGLWLESERLLHLRIDSIGVETQRSVVKEERKQSYDNRP
                     YGTIIEET"
BASE COUNT         6034 a         5892 c         5404 g         6063 t
ORIGIN      
        1 tgcctgtatt ttcaatcttg ttcaggtaaa tgcggcagga atcctttgac ctgagcgatg
       61 aatcaatctg ggcttgtaaa ccgaaaaaac aaaagattgt cagagatgaa caaaggataa
      121 aacgcttcat atcaatttca attcaagaat gagaaatatt atccggttaa aatattttca
      181 aatgtacaaa aaagacaatt gcacattaat aatgcattat tcgcaaatga atgcactgca
      241 acagtgcata tttacatggt ttcggcaagg ttgctcataa tataggcaca gcggtgatga
      301 accagcgaaa agggatggcc ctgtcaggat agtccgcccc atgaaggata tgaatgtggt
      361 aattggcagt ataaccctct gaacatccag ataggacaca acagtacgaa aaaaaatacg
      421 aaaattgtta tccggctatt tcatgatttc cttttcgatc acttcgccat agcgggaata
      481 atcatcatac agcgctttgt acttctccgc attcgcagga tccgggtgat actccgtttc
      541 gtagccgccg cccatatact tctgagcttc gggcacatct gtataaatcc cgcccaccac
      601 ggctgcggcc atggccgaac ccagcgcgca ggcctgctcg ctgcgggcaa ccaggatagg
      661 cttattgatg acatcggcca ccacctgcat gatgaagggc gacttctttg ccacgccgcc
      721 cagcgcaatg atcccctcga tgggaatgcc ttcgctgatg aaacggtcaa cgatcttttt
      781 ggcgccgaag gccgttgatt caaccagcgc cctgaagatt cgcggtgcat ccgagcccag
      841 gttcagcccg aggatagcgc ctttcagcaa ctggttggca tcgggcgtgc gacgtccgtt
      901 catccagtcg agggccagga ctccggattc gccgacaggg attttctctg cttcagcggt
      961 cagttgcggg atcagtttac cggatatttc ttcaatgagc ctggctttgg ccggatcatc
     1021 aatgacggat gtctttgcaa gcaattcccc gaccggccac atcagcaatt ttttaaacca
     1081 tgcgtaaata tccccgaaac ccgactgacc tgcttcgagc cccagcattc cgggaacaac
     1141 agagccatcc acctgtccgc aaatgccatg taccagttta ttccccactt cctccatggg
     1201 ggctatcatc atatcgcagg tggaggtacc catcaccttg ctcaggtaat aaggctttat
     1261 ttcaccacct atggcgccaa ggtgggcgtc gaaagccccg gcgcccacgg caaccttttc
     1321 gcttaatccc agccgtccgg cccattctgg cgtcaggttc ccgattttta catcgcaggt
     1381 ataggtatca gtaaacaggc ggttacgcaa cccggccagc agcggatcta ttttcaacag
     1441 gaattcttcc ggcggcagcc ctccgaaatc tttgtgccac atcgccttat ggcctgctgc
     1501 acaacggctg cgcttcatgg tgagaggatt cgccgtgccg gtgatcaggg ccgggatcca
     1561 gtcgcaatgc tcgacccacg aaaaagccgc ctgcctgatc ctttcatctt ccctgataac
     1621 atgcaatatc ttggcccaga accattccga ggaatagatg cctccttcat atttggtata
     1681 atccgtcccg ccccagtttc ttgcagttgt gttgatctgg tcagcctcgg cagtggccgt
     1741 atgatccttc cacaacacga acatggcatt ggggttatcg gcaaattcgg ggagtaacgc
     1801 cagcggagtg ccgtttttgt ccacggccac aggagtcgat cccgtggtat caaccgatat
     1861 ggccacaata ttcgcagcca cctcaggcgg aacttgtgaa atcacatccc tgacggtatt
     1921 ctcaagccct tccacataat cgagagggtg ctgccggaac tggtttttgg caggaacgca
     1981 gtattttccc tgcttccagc ggggataata aaaaacgctg gatgccattt ccgtcccgtc
     2041 ggcggtgtca accaatatcg tccgaacgga gtcggttccg taatccacac ccagggtata
     2101 ctttgtcacg ttggttcagg atttttttgt ttcgccgaga aatgctatta cctggttata
     2161 tacgttttcc ggggtgaacc cgaatttttc atccagcact ttcgccgggg ccgaatatcc
     2221 gaaatgatcc agtccccaaa ctctgccgtg agcgccgacg agcccggcca gcgtaaccgg
     2281 taaaccggct gtaagcccga atgccggtat cccgcagggg atcacctgtt gccggtattc
     2341 ttccggctgt tgccggaaca gtccttcgga aatggccgaa accactctta ctttcaggtt
     2401 gttccttgtt ttcagcaggc cggcgctttc gatgagcgtg gccacttcgg aaccgcttgc
     2461 gatcagcacg atatcgggaa cgcctccgcc atcaagcgcg atataggcgc ctttcgaagc
     2521 ctgctgcgcg tcagcatagc gtgttgatcc ggcttttgca ggaatatcgg cgatgttttg
     2581 tcgcgaaagt atcagcgccg tgggagaatt gttgttttcc atcgccattt tccaggccat
     2641 ggtggtttcg gccgcatcgg caggacgtaa ggcaagcagg ctcatgtgcc cgcgatggtt
     2701 cttcaggttt tcgagaagcc tgatttgcgc ttcctgctca acgggctgat gggtgggacc
     2761 atcttccccg accctgaaag catcatggga atatataaat ttgacgggca atcccatcag
     2821 cgccgataac ctgatcgccg gtttcatata atcgctgaac acaaagaagg tggcgcaggc
     2881 ggcaaatacg cccccatgca gggcaatgcc gttacagatg gcagccatgg tcagttcgct
     2941 tacgcctgca tgcaggaacg cgcccgaaaa atcccctttt gtgaagggtt tggtatactt
     3001 gaggaatccg tcggttttat cgctgttggc aagatcggcc gaggagacga tcatgttgcc
     3061 ggctttctgt gccagatagc caagcactgc cgatgaagcg gcacgggtag cgatacccgg
     3121 tttctgggct atttcgtcaa aatgaagttc cggcagttcg cctttcagat aacggtgaag
     3181 cgttgcagcc tgctccgtat gttccgactc ccattcctgc tgttgcgatt tgcggtaaga
     3241 ggccagggca atgtttttcg ccttcacttt ttcgtaaagc tcagccactt cggggaatac
     3301 ggcaaacgga tcctgtgcat tgccgcccag gtgctcaatg gttttttcaa acgaagcccc
     3361 tgattcgccc agcggcatgc cgtgggttgc acactgacgc tcgaaacttg taccgtcggc
     3421 ttttaacgct cctttgccca tgatcgtttt accgatgatc agaaccggtt tctctttttc
     3481 atcattggct tccgtcaagg cattccttat agccgatgca tcgttgccgt tgatggtcag
     3541 caccttccag ccccaggctt catatttttt tgcggtatcc tcacaagtta cggccctggt
     3601 ttcggtagaa agctggatat cgttcgaatc gaaaaacatg atcaggttgc tcagcccgag
     3661 ataaccggca atacgtccgg ccgcctgaga aatttcttcc tgtatgccgc catccgaaat
     3721 gtatgcgtaa gttttatgcg acatccattc gccgaagcgg gcgcataaaa acctttcggc
     3781 aatggcagcg cctacggcca tcgtatgtcc ctgtcccagc gggccggaag tgttttccac
     3841 gcccctggta acatccagtt ccggatgtcc gggcgtggga ctcccccatt gacggaaatt
     3901 cttgatttcg tcgatggtgt actttcccga aagggtcaat acggcataga gcatcggcga
     3961 catatgcccc gggtcgagga agaagcggtc gcgcatatgc caggccggat ccgaaggatc
     4021 gtaccggaga aattcggtaa aaagaatatt aataaaatcg gcgcctccca tagcgccgcc
     4081 aggatgccct gatttagctt tttccaccat agcagccgca agtatgcgga tgttatcggc
     4141 tgccctgttg atcgtttttt cgtccatagt aattatcata gtttttattg tccgtaatag
     4201 gcatttttac catgcttacg gttaaagtgt ttagcgagga gataatcggg gatcggtcct
     4261 gctgcgccca attggactga caaccaggcc attctggcta cttcttcgag cacggcggca
     4321 ttgtaaaccg ctttttcggg agtttcgccc cacgtaaagg gaccgtgatt gcccacgagc
     4381 acaccgggta catgcaaagg gtcaatatct ttgaaagttt caacgataac cctgccggtt
     4441 tccttttcat aggctgattc cacttcctgt tgtgtcagca tacgggtaca gggaaccggg
     4501 ccggaaaaat ggtcggcatg agtagtacca tagacaggta tcggtttgca ggcctgagcc
     4561 cacgccaccg cataggttga atgggtgtgc acaatggcgc ctattcccgg aaatgccttg
     4621 taaagctcaa tatgcgtgag tgtatcggag gatggtttca ggttgccgtc gacaaccctg
     4681 ccgttcagat caaccaccac catttcgaac ggcgtaaggt tttcgtataa aacgccgctg
     4741 ggctttatta cgatcaggtt ttcatccctg tcgatcccgc tcacattgcc ccatgaataa
     4801 aggacaagat tccgcttcac caatccgaga ttggcttcgt acacccgatg tttaagttcg
     4861 gtgagcatgg ccggtgataa ttttcaaatt tagggcaaat gtatcattgt tttataaact
     4921 acccggcttt ataaacaaac tccggtaaca acaacctgga tatcaccctt ataatttcag
     4981 attttcagtc atgacagcgc tcaactataa aaacttcagc gctccggcct gcatataata
     5041 caggtcattc cacttcaatt ccttccggaa ggaggagagg caggtatcct tatcgatatg
     5101 tacatactct attccggcca tatcgcagaa gtcttcgata tacctggcat ccacagcctg
     5161 gctgaaagcg gtatgatggg ccccgccggc ataaatccag gctgctgcgc ctgttttcag
     5221 gtcgggttgg ggaatccaca aggcacgggc gacaggcaac ttcggcaggt cggcatcggg
     5281 aacgacgcaa tccaccgaat ttacgatcat acggaaacgg ttgcccatat ccacgaggct
     5341 ggttgcggta gccggcccgg caggtacgct gaaagtcagt cgcgccgggg cttccttgtt
     5401 cccgattccc agcggatgaa cttccagttt cggcttgtga ccgctgaggg tcggacatat
     5461 ttccagcata tgggccccga ggaccttcat tcccgcagga tccaggtgat acgtgtaatc
     5521 ttccatgaag gagcagcctc ccttcaggcc ttttcccatt gttttgcata cgcgtaccat
     5581 ggcggcatgc ttccagtcgc cttcggcgcc aaagccgtaa cccgacgcca tgagacgctg
     5641 cgaagccaat ccgggcaatt gtttcaatcc gtgaaggtct tcaaaagtag tagtaaaggc
     5701 gctgaaaccg ccctgttcaa gaaaagcgcg gataccggct tcgagccggg cctggtaacg
     5761 gacattttcg tgatatttcc ctcccgtcat tccgtcggca gccacattat aaatctccgt
     5821 atattcttta accagatcgt cgacgaccgg ttcggggacg tcatccacga accttaccag
     5881 atcgccgacg ccatagccgt taaccgaaac gccgaattgt atctgggcgg caactttatc
     5941 gccttcggta acggctactt cgcgcatatt gtcgccaaaa cgggcaattt tcatatgttg
     6001 catatcgtgc caccctgcag cagcgcgggc ccaaacggcg atatcaccga ccacttcagg
     6061 atcctgccag tgacctacca caaccttgcg gttcatacga agccgggtca tgatgaaacc
     6121 atgctcgcgg tcgccatgcg ccgactggtt caggttcatg aagtccatat cgatttcttc
     6181 ccagggcagg tcgcggttga actgcgtgtg cagatgcagt aatggtttct gcaacttctt
     6241 cagcccgttg atccacatct tggaaggtga aaaagtgtgg caccaggtga tcagcccgat
     6301 gcaggacgcc gacgcattgg catcaatgca aaggttcgtg atatcatccg gggtcgtcat
     6361 gaccggctta aagacaatat ttacgggtat ttctgatgaa ttattaaagg ctgcggctat
     6421 ggtttccgag tctgcggcta cctgccggag cacttcgggg ccgtacaaat gctgactccc
     6481 ggctacaaac cacacttcca ggttgttaaa tgttttcatt gctgagtatt ttagatgatt
     6541 gtcaaaaccc tgattctaag attcaaaaac gtgtcatcgg gccgttatct caaggcagac
     6601 caccgattta gaaggcatgc tgaccttcac ttgtccattg ccggctttga atttgtcgaa
     6661 accggcggca atgactttcg gcgcctggcc aaaatcgttg taatcgttca tattttcgga
     6721 ggtaataacc tgccccgtca cggtcacttt ctgttcgcta cccaggttta cggttacagg
     6781 cgtttcattc ccggggtcaa ggtttaccat tgtaacatgg atacggcccg aagcatcctt
     6841 tgaggctgat acatttacag ccggaaccga ttcgtcgcct gaggcataat tccggcagga
     6901 aacggtgacc ggtatgagcg ttgccccctg gtgcaccttg tacatcctga acacataata
     6961 ggtcggcgtg agcgccatcc ggtccttgtc ggtcaggatc accgattgca gcacattgac
     7021 catctgggcg atattagcca tcacgatgcg atcggcgtgg ttgttgaaaa tattaaggtt
     7081 aatggcggcg accagcgcat cgcgcaacgt gttttgctgg aacaggaatc ccggatttgt
     7141 acccggctcc acgtcgtacc agtttcccca ttcgtcgacg acaagcccga ccctgtgccc
     7201 gggatcgtat ttatccatga tagccagatg cttgccgatc agctcttcca tccggcaggt
     7261 tgctttcagg gtagcgaacc agtccttttc cgtgaattcg gtggccgagc ctttgtcgcc
     7321 ccaaccgtgg caaagggtat agtaatgcag cgacaagccg ttcatcaggc gctgctccca
     7381 ccatgatttt tttgaccaca ggcgcatcat ggtctccgtc cagttgtaat cgtcaacatt
     7441 cggtccgacg gcgatgcgga agaggccttt cccgtcgtaa tcgcggcaga agttcgaata
     7501 acgcatcatc tggtcaaagt aaaactcagg gctcatggcg ccaccgcagc cccacgattc
     7561 attccccagg ccccagaact ttacattcca tgctttttca cgcccgccgg ctttacgtaa
     7621 atccgtcatc ggactgatat tgccggagtt gatatattcc acccattgcg acatttcctc
     7681 caccgagccg cttcctaagt tgccggtaat taccggttcg cagccaagtt gttcgcacag
     7741 gtcaaggaac tcattggtgc cgaaactgtt atcttcggaa acaccgcccc agttggtatt
     7801 gatcatggcc ggacgttttt cgcgctggcc gatcccgttt ttccagtggt attcatcggc
     7861 aaaacagccg cccggccagc gaaggttggg tatcgacata tcacgcaggg catttaccac
     7921 atcgcttcgt ataccgcgca cattcggtat ggaggagttc tcccctaccc agatccctcc
     7981 gtagatgcaa tgacccaggt gctcggcaaa atggccgtaa agatttttgc tgatgacggt
     8041 tcctgctttt tccggctgca tcgtgacagt gttttcctgt gcaattaccg ttcctccgga
     8101 aaccgcacaa ataaaggcca ggaagatcgt tgtgagtgaa cttttcattt tgaaaaaaaa
     8161 ataatgtctt gtcattgtta accgattcga gaacagttcc ggccttaccg ggaatgccgg
     8221 cagggattca tgcatttttt tctgtgcttt cccgacctcg ttttccatca aggttccggg
     8281 ggaattcaaa gtttgaatag gacaaatata agtgtatatt ttacacttca aagaaccggc
     8341 ttaaaaaata ttgataacca gttgcctgaa ttgaaaaaat atcctgaaaa aatatcggga
     8401 gctccgggaa atggccgtaa attcgggact ttaaagcaac tttcatgaat atcaaacaat
     8461 gaaatttttc aggttaccat tccttattgc cattctcact ttcacgggcg cacgatacat
     8521 cctggcccag gaaaccaacc gggattgtat catccggaat gagtttatat ttgattcagc
     8581 cccgtttgcc gagtgccatg cttcgacgat tgccgaaaca gccgacggac tggccgtagc
     8641 atacttcggc ggcactgccg aaaagaaccc ggacgtagca atctggatca gcctttacag
     8701 aaacgggaaa tgggatgttc ccgttgaaat cgccaatggc atcagtcccg acgggaaacg
     8761 atatccctgc tggaatccgg tactttaccg ggacggttca ggcgttttgc tgctgtttta
     8821 taaggtcggc cccgatcctg tcaactggtg gggaatgata atgaaatcgg cagacggcgg
     8881 aaggacatgg agtgttccgg cccggcttcc tgacggcatc ctgggaccag ttaaaaacaa
     8941 accggtaagg ctgcaaaacg gtgagttgct ttgcccgacc agcaccgaag ccggtgaatg
     9001 gcaggtttat tttgaacgca cagccgatct gggactaacg tggagtaaat caggatttgt
     9061 agacgatcca aggcatatgc aggccattca gcccgccatt ctctttcacg gaagaaaaag
     9121 actccaggct ttatgccgca gccgcggcaa tgtaattgct gaaagctggt cgaaggacaa
     9181 cgggaaaacc tggacgccgc tggcgccgac ttcgctgccc aatcccaatt caggcatcga
     9241 cgcggtaacc ctcaccgacg gcaggcaact cctggtctat aatccttcca atgttcccga
     9301 agggaaatgg acaggtccgc ggtcgccgat atgcatcgca atatcgaaaa acggaaaggt
     9361 gtggcataag gtcctgacac tcgacagtga accgggagaa ctttcctacc ctgccgtaat
     9421 tcagacatcc gacgggatgg tccatattac ctatacatgg aaaaggacga ccatccggca
     9481 tatcacggtt gatccggata acatctgaac cgttactgcc aaggctgtca acaatcagag
     9541 cgataaacaa aaagaggctg cttactctgc aacctctttc tgcacatcaa acaaattaaa
     9601 ccaatcaacc aaaacctgat agtaaaaact gctctatatt ctacatcaac cgtctatttt
     9661 aatattctga cgctgaaaaa agccatcgct ttgccggtgg tttcttcatc gtacagaagg
     9721 ttaagtcttc cgcttttcag gacatagtat ctgtaaacgc ggttaatggg ggcaatctcc
     9781 tggtttacag gatcatattt gttacaattg ctgcaatacc tgtaaagacg actgccattg
     9841 cgtgctgcaa tcagctccat aaaagctgcg gcaatggtat cgcctctttg attcagcatg
     9901 gggagcatat cgtactggtg gccattaatt cggtatgact tgatcgaagc gttcggaatc
     9961 tttactatct ctcccgaggt tgtcgtgatg cgtgtatttg taattccttt acgcattgtg
    10021 gtaatctgat aaacaacgcc gtcggcaatc acgaaattct cgataactcc tgtagcgaaa
    10081 acgctcatcg aaagaaatac caaaacaagt atggaagctg ggattttcat gatgcattcc
    10141 tttcctttac tgctttaaca aacaaaccga cctgttaata aatagcctca atgccaaaga
    10201 aaggcaaaac agtgggtgca ttttcctggt ctaccctcag gtaaaatttc ccgtctttga
    10261 ataccgtgta cagatactga gcttcggctt ttttatacga attgcttgcc ggatcgcagc
    10321 attcgccata tttgcaatac ttatataacc gaaggccgtt tcgtgcggta acaaattcca
    10381 gcaatgccgt gcagcccggt ttggcattgg gacacatcag gggcagccgt tcgtacaaat
    10441 ggcctttaca catgtaggcg tctactttat tgaaaggcac cttaagtgtc gtaccatcgt
    10501 tcaacacgat acccagtctg aacattcctg atttcagttc tttacagaaa taggttttac
    10561 ctttcacagt cacataattg acagtttcac cggctgatgc agccagcgct aaaaccatca
    10621 tgaacaaagc aataagaaac gttttcatgg ttatatcctc cttggttttt gggttaataa
    10681 ttttacatgg taaaagtatg taacaagctg atggttaaac aactatattc gattatccgg
    10741 ttaaaagcat cgttgaacag gtcaacgggg acaaatgcca ttttacagtt tgaaatgatt
    10801 tgtttaccga tcagaaaaat cgaaaaagaa aggattgtcg gttctgacgc agggtcggca
    10861 gagcgtgagc cgaaagggtg cgtggggaag ggaggattca gaatttgaaa atttaaaaat
    10921 gtgaaaatgt gaaaatgtga aatttgcggg ctgttttggt ttttggcctg ccgtcttacc
    10981 cacaaccccg cccaggcagg taaactttta gcttttagcg ttttgggggg ttgggttctg
    11041 tgttctgcgt tttgagtttt gagtttcgtg ttttgcgttt cgtggaagtc gtaattgaca
    11101 ttcgtggaat tcgtggttcg ttgttcgtga ttcgtgatta gtgttttgtc ttttcccctg
    11161 cccgctcact cacatccctc ccttggcagg taaagttttt cgttctccgt tttcccctgc
    11221 ctgccacctc caggctcgcg caaggcaggc aggcctgctt tcctctcaca caaagccata
    11281 caccggcagg taaagttttc cgttcttcct tatcctgagc acacaatgat tcatggctgc
    11341 gcaccgtatg aaaatggtgc ccggccgtca ggagaagcca ataaatgatg gggaattcta
    11401 cttccttttc tcgatgatat cgaagaagtt tttctgatag gtatcgctga tcggtatgcg
    11461 ctgatcggca atcttaatgt gcttgcgttc tacaaattcg atcttatcaa cggcgacaaa
    11521 atatgatttg tgaaccctgc agaactgggg ttcgggcaat acatcctcaa tacctttggc
    11581 attcatcagg gtcatgatgc gtttgttacg cgtcacaata cgccagtaat cgcccattcc
    11641 ttccacatac aacacctccg aagccgtcac tttttcgatg cgtgtttcgg ttttgacgaa
    11701 aaaataatcc ttatcaagcg attcgggaat atgaggggtt ggcagttttt cagtttgatc
    11761 gccatttccc agccgcatcc tgtcatacac cttgttgacc cctttcagga aacgttcgaa
    11821 tgaaatcggc ttaagcaggt aatcggccac atccagttcg aaaccctgca tcgcgtatga
    11881 gtcatacgcc gtggtaagga ttacgtaagg ctttacattt agtgaattca gcaactgcaa
    11941 cccggtcagt tcctccatct gaatatccag taaaatcagg tcgacatggt tgttccggag
    12001 gtattcgagt gtttcgatag cattatcgaa ggtattcagc agattaagga acggcacttt
    12061 tgtacaataa tccctgatga tatcgagcgc cagtggttca tcgtcgatgg ctatgcaatt
    12121 gattttcatg tcaattctca atttcaaggt taatacggta cttttcttcg tcttgcttaa
    12181 tgatcaactg gtatttgtcg gggtaaagca ggtcgagcct ccgcctgatg ttggccagcc
    12241 ctatgccgct tgcctgctgg accggggctg ccgtcgtttt ccgaatgtag ttgataactt
    12301 cgaaacgaag ccagtcctgc tcggtcttca gcctgatata aatgccgggt tcatggtttt
    12361 tgctgccatg tttgaaagca ttctcaacaa acgggatcaa cagcataggg gctatcgtcc
    12421 tgttatcggt attgccttcg atgctcaggc ttacaaaatc gggatgcttg atgcggagtt
    12481 tctgcaattc aataaaactt ttcagatact caatttcctt ttccagcgga actttgtcgg
    12541 aagtggcatc atagaggaca taccgcatga tggaagacaa tttcataacc gccgccggcg
    12601 catcttccga tttgcggtat acgagagagt aaatattatt gagcgtattg aacagaaaat
    12661 gcggatttac ctgagaacga agcaatgcca gctcgccggc ctgctgttgg ttaatgagct
    12721 catcctttaa tttctgcgtc tcgaaccaac tgatcatgaa gctgatgagt acggcatagg
    12781 ttcctgttat aatgaccagg cgcacgtcgc cccagaccca aacccatttg aaaatcaatt
    12841 cctgctgagg caggttgccg aaatatttcc agaaaagata atcggtaatc accctgaaag
    12901 ttgtaagcat agccagcatt accaaccccg tgcctgcagc cagcacttta ttcttcagct
    12961 ttaacaagcg gggaaatgaa aaataaatgg catagaaggt gataaggttc aggaaagttg
    13021 taatcagaag atttttaaga tactggttat caatcatgtt tgctttgtca agttgcccga
    13081 tataaatagg gtacagcgac tgattgataa aatagaacca gtagaggaga tgaataatga
    13141 tgaccgtttt ctttttcatt gcaacagttt gggagttggt tttgcgtatt ctaagttaca
    13201 caaaataata gctttcaatg cttcaaactg gcatcaaatc tatgaaatac attgattgaa
    13261 cggtaaaacg acaggttgaa tctgcaatga acaaagtata aaaaagtgac gcctgctttc
    13321 agccttttca acttccgccc ggctgaatta ttgcatttgc ttccttttac cggcctctat
    13381 tttcaccgct ttaacaaatc aataacaatt gcgctgaaaa aagcctaaca tctccacaag
    13441 ccatttgttg ataacagtaa aacaaatagc tatgaaaaaa ctgatcatga tttcgctttt
    13501 cctcgtggca gcgaccggat taagtcttgc ccaggaggat accgcccccc aatccatccc
    13561 gcttatcggg agtattgctc cttcctttac cgcccagtct acccagggaa ccattacgtt
    13621 tcctgaagat tacggacgaa aatggaaaat ccttttcagc caccccgctg attatacgcc
    13681 ggtatgctct tccgaaattc ttgcgatggc aaccatgcag caggattttg ataaactggg
    13741 cgttaaactc gccgtcgttt ccacggatgc gcttgataaa cattacaact ggaaaaaatc
    13801 gctcgaatcg gtccggtaca atggtcatga accggtcaag atcaacttcc cgctaatcga
    13861 cgaccaaaac aagtcaatcg cccggcttta cggcatgctg catcccgagt ccccttcatc
    13921 ccgcgatgtt aggggcgtat tcatcatcga tccggccgat aaggtggaag ccgtgttttt
    13981 ctatcccaac aatgttggcc gcaacatgga tgagatcaaa agaacggtta tagccctgca
    14041 aaccgccagc gattattcgg tgctcacgcc tgccaactgg aaacccggtg aagacgtgct
    14101 gctgccgtat ctcaaatcag tggacgaggc gcttgtccag tccgggaaag aaaaccccga
    14161 attttattca ctcacatggt atatgtggta taaaaaactg gatgtcaaat aacagccttt
    14221 taagcatctg attttcctga aaattacaga ataacaggcc tttgatgagt gctactaccc
    14281 ggggctgtcc ttgagtatcc gaagcaaatc cggccttaaa ggacagcctt attattgttt
    14341 tctgatcaaa atttttgcat tatttatata tctttgacat gcgtaagcca atcgctgagt
    14401 tttacaaatt tccggagagg caggataaac gcctatgatg gtaaacctgt aacccggttc
    14461 attttatctg atcaaaatgg aaaaagctgt taaaaatgcc atgagaggtc ttattatacc
    14521 ggtcatggtt cttattgcaa attctgcagc cggacaaggt aaaacgattc agattgattt
    14581 cctggggaat tgcggacttt tcatgactga cgggacactg aatatatgca ttgacttccc
    14641 gtataaatcg ggagcctatg gatacatgac atacaatctg aacttgccgg acagcattcc
    14701 cgaaaacaca atctatattt ttacccatgg gcatgccgac cattttgaca gaaaactatt
    14761 caaggaaaca aacaggcaac ttttcgggcc ctggccggtc aaagccttta tttcggggaa
    14821 aaggaagtta tcattccatg aattgaatgc catcagtcag aatttttcgg ttcaaaaatt
    14881 tacaaccagg cacaggctgt cgctgaagca ttattcatac ctgatcgaat gggacggtaa
    14941 aagaatttat atttcaggcg atgccgaaaa tgcagatgtc attggcaaca tgaaaaacct
    15001 ggaccttgtc tttgcaccgg gctggctgtt aaacgatgcc agtgacaaga acatagccat
    15061 tgatacaaaa aagatagtgc tctatcatca cgtgagtgtt gagggcatta ttaacaacag
    15121 caaagacaaa atcattgttc cgtcgcggag gcaacttatt gagctgaaat aaaacgatgg
    15181 gcaaacccac acgggcgcca tgttccgaaa gaacagggaa aactataaat gcgcctttaa
    15241 cagaataaaa tccgcaagat catgaaggca ggttaagtga tcgggcattt tatcggcaca
    15301 ttcctggttt tcgccatcat atttctcaac acaaacgata tcgattaccg gcagggaatg
    15361 attcgccaaa gcaattcagc attgaatctg agcttcggtc atcagaacgc ctccacagga
    15421 aaagaacata tccacaaaca tttaacggat gattttacag gaaacatatc acttacttga
    15481 aacccgctac aggaaatacc tggacaatgt cagcattgaa aaagtccagg ctggtatttt
    15541 cctgaccgcc gttcagttat caaccgggta ttgcggcgtg gcaagcacgg acctggattc
    15601 aaccgtcaat tgcagtagca ggcaaaacag ggatttcggc gatttcaccc cgggcaacat
    15661 caaagggcaa aatgtaaccc ggttatttca attcccaggc gattccagaa tccttgattc
    15721 cgtaaagctt gccgctttga atgccgtatc ggcaggtatc attttgcaat cggattacca
    15781 gataattgaa gacagggatc catttgacct gcttaacctg gacggacaaa aaaggatatg
    15841 cattgtcggg gcatttcagg attacataaa aaaacttgcc ggcagcggac atcagttaca
    15901 tgtgctggaa ttaaacaaag atgcgctcac cgaagagcag caacagtatt atgtacccgc
    15961 tgccgatgcc ccggcggtta tcccagttgc tgatattatc atcctaaccg gttcaaccct
    16021 ggtgaataat accctggaca acctgctgac gctgattcct gcaggaaagc aggcggttgt
    16081 ggtgggcccc agcagcagcc tgatccccga catacttttt aaatataatg taagtattat
    16141 cggagcaact aaaatcaccg accctgaccg gatgttcgcc attgtagccg agggcggact
    16201 gggatatcac ctgttccatt actgcgcgaa aaaaatctgc ctgatcaaca acctgccgcc
    16261 gtcagagcgg tgacgtctga ttgccggaag catgtcccgg ccggcctgcg ctgataacaa
    16321 gaaacggcac ggcaaaaaaa aaacgaagcc tttaagcttc gttttgagcg gaaaacggga
    16381 ttcgaacccg cgaccctcag cttgggaagc tgatgctcta ccaactgagc tacttccgct
    16441 tatgagggca caaatttaac atttccttgc gaaagagcaa caggtctgaa aatatttagc
    16501 ggtcaggcgc tgtaatacac tttatattgc catgccccca gggtaaaacc ggggttttca
    16561 cccagcgcta ccggcacagg cgggctgaac agttcataca cttcaccggg aaacgcgcgg
    16621 gtaaacaccg aaaacgaagc agccctcggg ctcagattaa ggatagccat aacctggttt
    16681 tcgccttccg aaaacttcag ggcgatcacc tggttatcgc agttggtggg tatgacagaa
    16741 aaatccgacg ggtcagctcc tctatgaaac acgggatgtc cgtcacgtaa tgcgatcagt
    16801 ttatcgtaaa cggcaaacat tttatcctcc ctccattcaa tcaggtcctt gtcgaagaac
    16861 ttcaggcgtt ttgcgtttcc ggcctcctgc ccggaataaa cgagaggcat gccgggcagc
    16921 ataaaaataa gcacggcaaa tgcttccaat gccactccca atctttcaat ctcgcttccg
    16981 ttccaggagt tctcgtcgtg gttgctaata aaaagcatgt tggccgcatg tttcggaaaa
    17041 ttaaaaatct cctgttgcag taacatacgc aaatcggtga ctgttttctt accctgagcc
    17101 atatcgttga aacaatgata caggttccag ttgtacaaaa catgaaacgc cttgtttacg
    17161 agttcgcgca attccgattc ggcaagcatg aacaggtcgg gtttcagggt aaccagcttc
    17221 tcctttgcct cttcccagaa ggacgtcggc accagatggg ccatatcgca acgaaagccg
    17281 tcaatacctg tgttttcgat ccagaaacac atatcgccga tcatggcttc acgcattccc
    17341 gtcgaattgt aattgagacc gatcacatcc tcccattcgg aaaaaggcgg cataaacttc
    17401 cccttgacgt cgagccggta atattcagga tggtcttttg tccagacatg atcccaggca
    17461 gtatggtttg ccacccagtc gaggatgaca tacatgccca gatcatggat ttccttaacc
    17521 agaccggtaa attcctccag cgttccgtat aattcatcaa cggccctgta attgcggatt
    17581 gaataatagg atccaaggct gcccttgcgg ttaacaacac cgatggggta caacggcatg
    17641 aaccatagaa ttcccactcc aagtttcttc aggcgcggca agtgttccct gaattccctg
    17701 aacgttcccc cgggagagta ttgtctcagg ttaacttcgt aaatagtaag gttgcggctc
    17761 cagccgggaa gctggtttat cgttgaaatc atgctccgat cattgtttaa aggtggaacg
    17821 cctcaagcga attatcaaaa tcaatgtaaa aatcagcttc cggctttagt gattgaacca
    17881 ccagatgctc cctttcaagc actttcatcc tgaattcgtc aagttcttcc ttcttctcgg
    17941 caatttgctg ctccagcgtt tctttatagg tgagttcaat gaacaccctc aggttggctt
    18001 cctgaatctt aacagaatat aatccattga taataagcat atctatttcg gaaaaatccg
    18061 taatgagctc atccacctga agggttacaa ggtcaacaca gggcaatgag gattttttgc
    18121 cgttcctgaa ttcagcgatg ttcttcctga tacgcggcca gtcgtattca tcatagccga
    18181 tgctgccgat gccgtgtttc attctccacg cggtgcgctt ttcgggagga attgtatagt
    18241 aatcgtccat atgaaggatt ttgcacttca cgccatcttt cctgtacaga cgcgccaacg
    18301 catttgagat ttcgcatttg cccgaggaca cttcgcccga taacgtgact atatatttcg
    18361 ggaccctttc ggtattaatc ctttcataga cggataatgc cgcctcttcg tgctttttat
    18421 tgattgaaac aagatcgttc agcatggcta tcggtttcgt ttaatttttt acccgtgtta
    18481 tccattgagc aggcgagatg cctgtatttt ttttgatccg ggtacttttc agaagttaca
    18541 aatttacaat taaatgaagc aggaaagaaa gccagcgcat ccggcatcct gcaacgtccg
    18601 ggcaaacgcg ttttccctga attcaatatt tgcagtactt ttggcgtctc aaaaccgaaa
    18661 atttgccgac aaacaagcat acccgacgta ttatcctaag ggctttcctc ataccttccc
    18721 tcctgctttt tgcgttatgg atcattattt tttcaataag aacggtattc cgcaaagggc
    18781 ctgaccagcc tggctttttc cagggcaata cagccttcgg tttgacgatc cccggcaaat
    18841 acgcggtttt cggcatcgac gtttcacatc accagggcag cgtcaactgg agcaaggtaa
    18901 aggaaatggt atccaacaac aggaaagtta cctttgtatt tatcaaagcc accgaaggga
    18961 taacccggca agaccagaat ttcgaagaaa actgggaagc cgtcagaaag gagggtttta
    19021 tcaggggcgc ttaccatttt ttctatccgt cgcgcgacgc cggccggcaa gccgaaaatt
    19081 tcatcagcca ggtcagtctg tcgaaaggcg atctgccacc ggttattgat attgagcata
    19141 caaacggacg gacgaaaaaa cagatttgtg aaggcctcaa ggcatttatt gacgaaatcg
    19201 aagcagaata tgatgtcaaa ccgatcattt ataccaatat ttcatattac aacaactatc
    19261 tgtccgacga cttcgccgat tatccattat ggatttcagg gtatcacggc gaagacaaat
    19321 tcagggagaa attcaaatcg ggctggcatt tctggcaata cagtgaagac ggccaggttg
    19381 acggtatcaa aggcgaagtg gattgcaatg ttttcaaagg gtcggagagc gatctccaaa
    19441 aaatgtgcat aagataacca ggctgaggat tatcgccctg aaaaccggaa tccggaaccc
    19501 cgggcacgac atttgactgc tgcctcgcgc tgatcgtgga ttaaatttca tccggcggat
    19561 cagcattccg gctccaattc ccaggcggtc aggaaaacaa aaagtctccg tgatggagac
    19621 ttttcgccag aaaaatatat aaatgttact actcttcgta tgcgcccggg tcatcgccaa
    19681 agcaaatgcc gactccacgc gccgttttga taagcgcggt gtcgagggtt accaggttgg
    19741 gttgttgaat ggcatccttc agggttacac tggtgatata aggatgacgg tatgaaacca
    19801 tcctgccgaa ttctccctta aggaccagtt ccatggcttt tacgccaaac tgggtagcaa
    19861 gaacgcgatc ataagcaatg ggaattccgc cgcgctgaag gtgaccgagt acggttaccc
    19921 ggatatcgtg ttcacagccg gcacgctgaa gatcttcggc cagtttaaat ccaacgcctc
    19981 caagccttac attttcatag ccgacttcct gtgctttatg gccgacaacc ttgccgtctt
    20041 ttgattttgc tccttcagct acaacaacga tggcaaatcc gcggtcgcgg tggaaacggg
    20101 aatcgatctt gctctttacg atgcccaggt catacgggat ttcaggaata aggcacactt
    20161 cggcgcctcc ggcaacggct gaattcaagg ctatccagcc ggcatcgcgg cccattactt
    20221 caagaatgaa aaccctgtta tggctggctg cggtagttac gagcttgtca acagcagaag
    20281 ttgcttcctg tatagccgtc tggaaaccga aagttgaatc ggtagccgaa aggtcattat
    20341 caatggtctt ggggacaccg ataatattga gccccttttc aaataattgc tgcgaaatcc
    20401 gctgcgagcc gtcgccaccg atattgatga cggcatcaat gcccaggtac tggatcttcc
    20461 ggatcatctc gtcggaacgg tcaacatatt cccatccgcc atcatgcttt tttacgggcc
    20521 aggcaaatgg gccgccttta ttggtagttt gtatgatgga tcctccctgg taatgaatac
    20581 cggcaacttt ggcatcatcc agcatgacga cttcggtggg ttcatttaaa attccgtcga
    20641 atgcctttat acatcccagc acttcccatc ctgactcctg cgaggcacgc ttaacaatgg
    20701 cccttatcac ggcgtttaat cccggacaat cgccaccgcc tgtcaatacc attactctct
    20761 tcatattatt tcaattttta tcttcttgat tagcaatcag ataaatccaa tcttctgttc
    20821 atgaattttg cggtgcaaaa gtacaatttt tatgcatttt ctttagcagt aattattcaa
    20881 ataactataa agtaattccg atccccgttc gcctgaaaga gcctgttcat tcttcgcggt
    20941 attcccgttg aaccggtcgc catacaacgg cggccatgtt aagattttgt taaatcccta
    21001 accccggtgt aaccaaatcg cctttcatcc gtctttttag tatacaaacc caaaaaacaa
    21061 caagtatgaa aatcataatc tccgccttca tccttacggt tctcatctcg cttaacgtga
    21121 aggctttcac cccttccgct ttcctgccca tggcagccga aacctatgtg aacgacatcc
    21181 cttttaatac cgctttaatc gcaacattca cccacagcgg gcgttccttg aattcctgta
    21241 tccggatcgc agacgaacct tttgtgaatg atattccttt cgatacacgg gctgttgcag
    21301 aaaaagaaaa acggaacctt cctgtacttc catacctgca accggctgac gaaccttatg
    21361 ccgacgacct tcccttcaat actgcagcca ttgtgaaact cctgcaataa aattaataac
    21421 cccgcatatt tccagcgcct cctgatgctc aaacatgaag aggcgctttt tttacatatt
    21481 gcccgtgtta aggaaatcaa aataatccgc cgcgtcttct ccgaatattt caaccgcctt
    21541 cccgatgatt ttatacgtac tatgctgccc tgcaatgcgc gagaaatgaa tttcatattc
    21601 cgtgtctttc tcgtcgagca tcagcacttt agccaaatta ggattgcggt cagagaccag
    21661 tttcttattg cagaaagggc aaaagaaatc aaccagttcc cctttcccta tccggaaggt
    21721 agggtgtttg attaccgaat aatcgccaag tttcggatgc agcataataa tgccttcttt
    21781 cccgatgcgg ttacgggtgg aaaaaacgac gttttcccct acattcagat aatttcggca
    21841 atacgggcaa agaaaattat ttgacatggc ataaagattt taatgtataa agatacaaaa
    21901 gtatcaggcc tgaattcaag caaaccaatc tcctgcaaag gcaaataatt acctttgccg
    21961 attaaatgat tacattaaaa catcttacaa actaccctga ccggattaaa aagcatgaac
    22021 caaaaacact gctatgccct ggtattcgat atggatggcg tgatagtgga caaccatcaa
    22081 tggcatttcc gcgcatggct tgaattttgc cggcagcatg gcttccggct gtccgaggaa
    22141 gaattttaca ccacgatctt tggcggcacc aaccgcgacc tgttgacccg cttatatggc
    22201 aacacattga ccgatgcgga aatcctgcag atgggcgagg aaaaggagct gctttaccgc
    22261 accgtgtatg aacctcatat aaagccggtg ccgggtgtaa gggaatttat cctttcagcc
    22321 cgtgaacgtg gcatcaggac agcgctggcc acatcagcgc ccatggccaa cgtaacatat
    22381 accctgaagc acacgggact tgaaaacaac tttgatgtga ttgtggaaag ttcgcagatc
    22441 gcacatggca aaccgcaccc cgaaatatat atcagggttg cctctctcct gggactcaga
    22501 actgagcgat gcgctgtttt tgaagattcg ctgccgggca ttcagtcggc aagggaagcc
    22561 ggatgcaaag taacgggcgt cgccactacg ctgcaagcat ccgaaataag tcatgccgac
    22621 ctggtgatca gcgattttac cgatcagcag acactgtttg ccgtcattga ttcctggttt
    22681 gtaaacagaa tgtagcccgg caacggccga agcctgtcaa ctgccggttt tcaggacggt
    22741 gtaaatacaa ccctgcgatt tcaacggaca ataccggaag cgtagctttg tggatcggat
    22801 aattcttcgt tattttgttg ccaaacatac cttaatccaa tctgataaaa aaaatagaat
    22861 cataccagtt ccatctttaa cacaattcaa atgagaatca agcttttggc tttactgtcg
    22921 gtatttctga ttaccggctt aacctatgcc cagcagggca acatcaaatt caccgaatac
    22981 gatatggaca acggtttgca cgttatcctg caccaggaca acactacccc tatcgtagct
    23041 gtcacaattc tttaccatgt aggttccaag aacgaagttg aagggcgcac gggttttgcc
    23101 catttttttg agcacctgat gttcgaaggt tccgataaca tcccccgcgg agagtatttt
    23161 aaaattacgc aggccgccgg tggaacgctc aacgccaaca cttcggaaga caggacgttt
    23221 ttctttgaat tgctgccttc aaaccagctt gaactgggat tatggctcga atcggaaagg
    23281 cttcttcacc tccgtattga tagcatcggc gttgaaaccc aacgctcggt ggtaaaggaa
    23341 gaacgcaagc agtcgtacga caacaggcct tacggaacga tcatcgaaga aac
//
LOCUS       DAOPYV010000045        23104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_2231_length_23104_cov_14.350644, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000045 DAOPYV010000000
VERSION     DAOPYV010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23104
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2231_length_23104_cov_14.350644"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            661..1107
                     /locus_tag="PKI35_07360"
     CDS             661..1107
                     /locus_tag="PKI35_07360"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNX44237.1"
                     /translation="MALTVYSDESFMREALKEAHKALEKDEVPIGAVIVCANRIIARG
                     HNLTERLNDVTAHAEMQAFTAAANFLGGKYLEECTLYVTVEPCVMCAGASFWTQIGRI
                     VYGAGDDKRGYSLVSSAILHPRTSVTQGVLAEECSALMKDFFRKKR"
     gene            1183..2010
                     /gene="rhaD"
                     /locus_tag="PKI35_07365"
     CDS             1183..2010
                     /gene="rhaD"
                     /locus_tag="PKI35_07365"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HNX44238.1"
                     /translation="MDFKTINNDITRITAEISETASYLWQKGWAERNAGNISVNITEF
                     ITHDMRELFTQGKYPFQPLEAPHPGLAGYSFFVTGTGKRMRDVANHFDRNVLLIVISD
                     DGKGYFTVKANSEQTGLTPTSELPSHLSIHGQLVKEGRRERAVVHTHPNELISLTHIP
                     RFCNQDSLNNLLWGMHPETMVVVPEGAGFVPYLLTGSLEIAEATLQKFKHHQVVVWEK
                     HGCFAIGRDVFEAFDLIDTIAKSACIYFQCRASGHEPEGIKPEHLADLRELSRKFRS"
     gene            2064..2282
                     /locus_tag="PKI35_07370"
     CDS             2064..2282
                     /locus_tag="PKI35_07370"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HNX44239.1"
                     /translation="MKQKHILSDGKRTIIWKGEQCMHKALCLRGILEDMDIKENTIHS
                     ISSAQVSYLLHRIEICPSGAIVNAESAE"
     gene            2352..2642
                     /locus_tag="PKI35_07375"
     CDS             2352..2642
                     /locus_tag="PKI35_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44240.1"
                     /translation="MGDIKIFCPVCGWEPDGGAYWQCSYCHTVWNTFDTAARCPGCGF
                     QYKYTQCIEHMGGCPAESLHIDWYHGLDKIVEDALKTEPVSIERKELTPVNK"
     gene            2651..4681
                     /locus_tag="PKI35_07380"
     CDS             2651..4681
                     /locus_tag="PKI35_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44241.1"
                     /translation="MKKLTLIIALLALATLADAQWQSNAALNTPIGVYSGEQVVTKVA
                     THSSGITYYAWFSLEGGNYNVRLQKTDVYGNIAFGSAGLLVSNHTSDSWITDFDMTVD
                     NDTCAVIAFQDIRTGNNDVFAYRVSPSGEMLWGNDGIRLSTSTDADYTPVVTVTDEGN
                     AVVVWEESSSVEKVRVQKISPSGSKLWGSSGITLQGAGTESYTFPRVFAAPDDQAFVL
                     WFKKDAGLYSPKHLYAQKISPDGSPVWSSDVVIFDGSGVSMIPTIDFVPDGSNGFYVA
                     WYDDRNNDWNYSTYVQHIDGFGQVQMTVNGVEAIVNNSNNHMYPSITLSPDGQDLFVF
                     VSEMDADQEMRGLYGQRISSSGQRLWPNTGKMFIGLSSLDVGMLKAQMADTNIVVIYC
                     KNNSFGGDQVKAMKIDPTGAYLWPSGGHIVVSSSNGSKSDLVAGVYNSGQFIAAWSDQ
                     RSDAGDIYGQNITTEGTLGAQQFALVLSPDTVIFDQSCSPFMVGKYFSIKNPMSYPVS
                     IDTLDLDAFLPEGQHWYVDTVLSFPVVIPPADSLRLNVKLDWIIKDDFGGYVTDTMNI
                     FSSVGPYREIIMIDSAYLWSAIDKAEKSNPLTLYPNPATDLVRIRFTGNDPVESVVIT
                     DPSGRVVKQAAVPASPAPTVQFSVAGLPSGEYLVRVYTQRGKPETGKLIVRH"
     gene            complement(4915..5463)
                     /locus_tag="PKI35_07385"
     CDS             complement(4915..5463)
                     /locus_tag="PKI35_07385"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNX44242.1"
                     /translation="MKYKKRQEWLLDSEQTLATFSGRLLAMLIDFVVISCIFLALARL
                     SYWLGLKIDNITMKNLFHIEIEAKKYSENKVQLLKILFGFIPTIYFTLTTFITNGQSI
                     GKKILRIKIVSFYHRRLNLWLCVERSLGYIASTLELGLGFIQIFWNPNRMTLHDKIAE
                     TVVIKTQKPAKKHLLKTSIASK"
     gene            complement(5522..6154)
                     /locus_tag="PKI35_07390"
     CDS             complement(5522..6154)
                     /locus_tag="PKI35_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNX44243.1"
                     /translation="MKHEWKKNEKQFYLPKNKPELINIPKFKFFTIEGNGNPNDDFFA
                     EYIGVLYSLSYGIKMSPKKGPEPKGYFDYTVYPLEGVWDINDSAKQSFNGTINKNDLV
                     FNLMIRQPDFVDNDFALQILEQTKKKKPHQLLDKVRFEEIVEGDCIQMLHIGSYDDEP
                     KSFKIMENFAEMDRLERKTKTHREIYLSDARKVSPDKLQTVLRFSVGKIK"
     gene            complement(6402..9098)
                     /locus_tag="PKI35_07395"
     CDS             complement(6402..9098)
                     /locus_tag="PKI35_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HNX44244.1"
                     /translation="MFSRPVFLKMFVLQVLLLPLIVKAQQPLFLSPQPQEIKTGTGTF
                     DLAAKPDIYISNAGYAETAFSAGQLQQELKDGPGLDAKIVTKPKKAGILLGLTGRDRK
                     IDELLAERNLTLPDKATEEGYILCTGEDVIFIAARTEAGLFYGVQTLKQLIRANRTGN
                     SIPALTITDWPALRYRGWMDDISRGPIPTVDFLKDCIRKMSEFRLNFFTLYTEHTFSL
                     KKYPDIAPPESLTAEEIAELTAYAAQYHMDVIGNFQSFGHMGKILANPFYSGLRENGD
                     ILNPASEDTYRFLGDVYSEIVPAYKSPFFNINCDETQGLGEGRSKRMADSIGLDGIYA
                     YHINRIDKLLKPYGKRLMMWGDIAVNNSGIISKLPKDLVILSWGYHAAESFDDAILPF
                     KNTGFDFMVAPGVSCWSELWPGMSNAAININNYVRDGAKLGAIGMMNTAWDDNGHNLF
                     NYNWHGLAWGAECSWRPAPPSTGELAVRERNEKLAQFNRSFDRLFFGAEGITPALFAI
                     DSLRLLPVRGIVGEGAFWQDLLDFNLENTSEDYGDKNLEVVTRANTLIQKIQQLKSDA
                     GRNRYLLDHAVFALKRVIFSADKNLARIILYQAKESGSEYAVLLAKPELEKLKNELYD
                     IKKEYIRLWKMENRDWWLDKNLNDYNRLARQCDDADKVVFIEPQPEMIDGSRPVILST
                     LFSDQILVYTTDGTEPTVRSEVYTGPLMIDSKVVIKAAVKTGNSLGVVSQQSVLVHKG
                     IGCPMKIKAAYSTYNPAYAAGGDQALLDGLTGSLNFADGRWQGYQGQDLELEIDLGKT
                     TPVSSVSMDFLQNAYSWILLPKDVQIFISGDGSDYRLVSTLTHDVPPTGQTTIIHTFK
                     AGFENMSTQYLKIVAHSRGNLPEGHHAAGNPSFLFTDEVIIR"
     gene            9258..11201
                     /locus_tag="PKI35_07400"
     CDS             9258..11201
                     /locus_tag="PKI35_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HNX44245.1"
                     /translation="MKKLLILSAIILGFTIVQAQSVTDLIARSSYPGTGMVVVFDSTR
                     VQVEESGLSHVFTHRLYKVQTAAGAKSLSVIKYDYDPLSAWSQIRRAVIYKADGSQVV
                     LDTAKVMDYPQPARAIYWGAREKMLAVGKLEPGDALEVFLYRKGFTYALLSDGSGDDD
                     RYAPPMRGHYYDIVPFYSNDPILVKSFEVSIPRTKTLQYEVYNGEVTSKAWIAGDRMV
                     YTFSCKDIRPWKSEPRAVDASDIAPKLLLSTSPDWKAKSLWFYSVNEEYGSFKSTPEI
                     QQKVNELLRNARTEFDSVSILTHWVADEIRYLGLTMGKGEGYTLHKGEMDFADRCGVC
                     KDKAGTLITMLRAAGFEAYPAMTMAGSRIEYIPADQFNHCVTVVKSRDGQYHLLDPTW
                     VPFLREVWSSAEQQQNYLMGVPEGADLAETALSDPGKHYLRISGMSEILPDGTLLGEY
                     SVTAEGQIDASIRSIYTRGYIADRAAAIEKEILHVSPQAQLLKINCDDPTNYMAGPIN
                     MHFSVKIPDYALVTDNEVIFTPFTIAGFFKRAQPQVYFETSPEKREYPFRDRTSHAVS
                     ISEKISLPGTATPVSLPSNETDTGPGATYRGEYVMDNGKLSVNFTAQFPKRIYQPEDW
                     PSFREAVEYQNKLAETPVRLKLK"
     gene            11240..13069
                     /locus_tag="PKI35_07405"
     CDS             11240..13069
                     /locus_tag="PKI35_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNX44246.1"
                     /translation="MKYQNIIPIIVTLALALPALAQEPASDAVYHQQTREYTLNHDGS
                     WTYHYSHSLKINTYLAFHNLYGEDFIVYNPNAQKLKINKSVTTTPAGKVVPSPDNAFN
                     ELLPGSCAGAPAYNDLREMVVTHTALERGAVIDFDYTLATQPSYWPWMAANDVLCTNS
                     PIEKLTYIVHVPAGITLNYSQYNITQQPTVIKSAGKTTYTWVLTDIPAAMREDFRSRE
                     QLGRPRIVFSTAKSLSETISGFTSQLAFTLSTSDELKTVAAKVRDEKKEDLAVALRLQ
                     DIVANEINYYPVPLANTGKDVRLAMETWQENGGTEIEKAVLLATLLKNAGITAEPVAI
                     FPESYYDEKSGNLFCIEKYLVSAAIGKNENILLSPLQTDSYDAGLALPGKKMVTLLNG
                     NKFRSEKISSPLPAISLTGNLSLDDSMKLTGNVDLQLTGKLNPYFTLSADSSRVKKLL
                     NGAFGEKTVAKYTAGELTAKKTGVQYTIENKDGLKEVSGLYFLRIPVLASGVDSWHMT
                     ELVSQRVEPLEIPFRIRESCQYAIALPANVKVLTKDADVNLKNDMGSIRIMIKQNGRN
                     LEITREIALNQTIVNPSRYGSFRDFINTWNNRKYAEIVLEKNN"
     gene            13094..14740
                     /locus_tag="PKI35_07410"
     CDS             13094..14740
                     /locus_tag="PKI35_07410"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNX44247.1"
                     /translation="MRTAILLSIFLCFWSTLLTAAPEGDLVSFTSSNLPIVIINTHGL
                     TIKDDPKIKVDLGIIDNGPGVRNNVSDPLTFSGYCGIEIRGSSSQSFPKKSYGFETWN
                     AIMQTVDTSLMGMPSESDWILNANYSDKTLCRNTLAYQTWMNMGHYATRYRHVELVIN
                     GEYQGIYIFSEKIKRDKNRVDISKLKPDDITGDQLTGGYIIKIDKTTGSGGGGWTSNY
                     LPLAHPSGQTIYFQYEYPKDDEIVEQQKYYIQQVIDTFETVLASPYFSDTLIGFRKYA
                     SENKLIDYFLVNEISKNVDGYRLSTYFYKDRKSKGGKLKMGPVWDYDIAWHNADYCGG
                     DLYSGWAYQFPCMDDYYQVPFWWGRLFDDALFKDRLKCRWQLLRQTYLSEEYIDGYID
                     SIASGLDEAQARNFIKWPILGVYVWPNPQPIPATYAGEIESLKAWIGNRLNWMEAHLP
                     GTCSTTAIAGAPQAEGTFASYPNPVSGVLTLEIDIPAATKVTGTISTIEGREIMRFTD
                     GLREAGFSYDQVNMQQLKPGMYLLKVIIGNTVHVKKIVKS"
     gene            complement(14746..16707)
                     /gene="dnaG"
                     /locus_tag="PKI35_07415"
     CDS             complement(14746..16707)
                     /gene="dnaG"
                     /locus_tag="PKI35_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNX44248.1"
                     /translation="MIPHETIDLIMDAVRIEEVVSDFVSLKRRGSNLIGLCPFHSERT
                     PSFSVSPVKGIYKCFGCGKAGNAVNFMMEHEKFTYPEALRYLAKKYHIDIREEEESAE
                     DIAAKSEREGLFAVSEFAQKFFSGQLTGTDEGKSVGMSYFRSRGFSDDTIAKFQLGYC
                     PDEWDSLTKQALLNGYSREMLDKSGLSIAKEDKLYDRFRSRVIFPIHSQAGRVIGFGG
                     RILSSDKSKAKYVNSPESEIYNKSKVLYGIAFAKNAIAANDMCFLVEGYTDVISMHQA
                     GIQNVVASSGTSLTVDQIKLIKRYTPNITILYDGDPAGIKASFRGIDMILEQGMNVKV
                     VLFPDGEDPDSFARTRRSSEVTEFISSAATDFIRFKIDLLLDETGNDPLKKVHIVKEF
                     INSIALIPDRLTRMAYVKESAERMKMDEAVILNELNKVLRNKFRKEAEMTKEEMEAAI
                     PEPPVIPAEQPSTLDLNSTEFQEREITRLLLAYGFRRIKMYRLAEGQSEPEEVFTSVA
                     EFIVNDLRNDGLELTFENYVKVFREFEKLVDSETEIKPEMMTQHADPAIARCAVDLLA
                     SPYELHDWSKRQIHIPLEEDEEVLERAVTSSLLAFKARRIEQLLAETGKEIKDPEPDT
                     DVMSLMRRFKDLKILNAQINKQLGRIVTR"
     gene            complement(16817..17194)
                     /locus_tag="PKI35_07420"
     CDS             complement(16817..17194)
                     /locus_tag="PKI35_07420"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HNX44249.1"
                     /translation="MTARIKQGQYFLNLLQAIIKMNTRFIDIIQLIKQSRANAIRAVN
                     AELINLYWIIGEYVSKKIEQSEWGESVVTELAKYIRQNEPEIKGFSDKNLWRMKQFYE
                     TYKDSPKLSTLLREISWSHNLAA"
     gene            complement(17257..17952)
                     /locus_tag="PKI35_07425"
     CDS             complement(17257..17952)
                     /locus_tag="PKI35_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44250.1"
                     /translation="MLIIADKRIPGQAKQKLSAFGELMEFSTSGITYDAISGHPDIFF
                     CQTPHGLVAAPNTPAGFLRQLDTKNISYTTGEQPVGNRYPGTAHYNAVVTEKFLIHNA
                     SVTDGKLRKLLLTFDILPLTFKQAYTRCNLIPLSEQHFITSDRGIEKVLRNHNLEALY
                     VSPKGIILPGFPNGFFGGCCGVWQDKLFVTGSLNRYAEGEKVRQFTLQAGLQVVELYN
                     GPLFDGGGIFFYD"
     gene            complement(17965..18990)
                     /locus_tag="PKI35_07430"
     CDS             complement(17965..18990)
                     /locus_tag="PKI35_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX44251.1"
                     /translation="MTPRHFAIPVFVPELACPNRCVFCNQRNISGTMAMPQPDEVAGI
                     IEKHLATIPPDSETEIGFFGGNFTGIEPSLQQSFLEIAEKYVRAGKAKSIRLSTRPDY
                     IDDRRLSLLKQFSVQNIELGAQSLDGEVLRLSGRGHTVYDVEKASELILENGFRLGLQ
                     MMTGLPGDTPARSVATARKIVALGASDTRIYPTLVIRDTELEKMYHAGSYVPLSLDEA
                     VESAAVIAGIFEEAGVKILRIGLHPSEGLLSGESLVAGPFHVAFGELVATAQWSAIFR
                     QKLAGNSSGVNENLLAEVPPGQINAAVGHKSLNKKMLLTRFRKVVFRENKELSGKQCR
                     FIVSSTD"
     gene            complement(19142..19909)
                     /locus_tag="PKI35_07435"
     CDS             complement(19142..19909)
                     /locus_tag="PKI35_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44252.1"
                     /translation="MRLLNKFLLIVAVSISVTDGFSQTLGLFYSRNQEFIEFQNQQRF
                     YSILEEGTKISDKKLVFRSGFYERNYDTLFLIPNNDSSFRIANVLTSEVNTKDSLCSI
                     FFYNMSRYIEIDLVIELFNYENVKYKELYMKNRDSVNLSVDGLYKLNIFCMNDRDKFE
                     YSLYNDDLLGKRILIQTFDQPPLKNNFYLIKCSSKSSFTLIGPVYIDYKQRNRRKWLN
                     SFRNWPWEWSLKKEHWYDPIERNMNMQSSNKKYFLSK"
     gene            complement(20434..21135)
                     /locus_tag="PKI35_07440"
     CDS             complement(20434..21135)
                     /locus_tag="PKI35_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNX44253.1"
                     /translation="MLKWLRITLISLGAIFLVSLIFAFTTAPFYAMYKLAVARGKPAK
                     EPVAVILLGGAGMPSENGLMRCYYAAGCAARFPESKVVVAIPGDLSDSADSPVLFAKE
                     LMIRGVPDERISFENKGRNTRQQALNLSKRWQGKGLSKPIILVTGPEHMYRAIACFEK
                     AGFTYVSGAPTFESSIDENQMQFEDKELKGNRNIPAIGHRPQVRYQFWNHLKYEVNVG
                     REAFAIAYYKLKGWI"
     gene            complement(21199..21708)
                     /locus_tag="PKI35_07445"
     CDS             complement(21199..21708)
                     /locus_tag="PKI35_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44254.1"
                     /translation="MKALSKIHLFVLFILSASLAGCIDKVEYPEVPAITFKSYGVMSG
                     SDGYDSLAVLTISYTDGDGDIGLYDYDTVEPYKYDYFLKFLEWRDGSFQEVALADTNF
                     TFNSRIPILTPEGKNKNIRGDISMYLEMYYAFMALQSDTIAFEVYIQDRALNKSNVLR
                     TPPFLVNRP"
     gene            21748..22737
                     /locus_tag="PKI35_07450"
     CDS             21748..22737
                     /locus_tag="PKI35_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNX44255.1"
                     /translation="MDVHAIRKQMFAITNDRQFNDVALEIFHYQYGQNPVYRQFVDGL
                     GVNVNDIRSYGQIPFLPVGFFKTHRVVSGSFGEEAVFESSGTTGSATSRHYVADFSLY
                     EESFTRTFNLFYGDVAQYNIYALLPSYLERKNSSLVYMVDRLLWKSNREYGGFYLHDL
                     EKLAAEIREALSENKKVMLLGVTYALLDLAEKFPMPLTGAVVVETGGMKGRREEITRS
                     ELHEKLMRAFATDSIHSEYGMTELLSQAWSKGGGLYACPPWMKVLISDTNDPLSLLPA
                     GRTGGINVIDLANLHSCSFIATQDLGKLNNHGQFEVLGRFDSSDIRGCSLLTV"
BASE COUNT         5776 a         5895 c         5527 g         5906 t
ORIGIN      
        1 cgaatgaaac acgaatgaac acgaataaat gttgtgagaa taacggattt cgtgagaatt
       61 cgtgtcacag gcgcagcctg tttcgtgaaa ttcgtggttt ttgtttctcc acgaattagc
      121 acgaatgaag cacgaatgaa cacgaataaa tgttgtgagg ataacggact tcgtggaatt
      181 tgtgtcccgg cgcagccggt ttcgggcaat tcgtggtttt tgtttctcca cgaattagca
      241 cgaatgaagc acgaatcagc acgaataaat attgtgaagt tctgagtttt gagtttcctt
      301 tgacctttgc cctgccgtct tacccacatc ctcgcccagg caggtaaact tttgcttttt
      361 gacttatgac ctttgacatt gccctgccgt cttacccaca acctcgccca ggcaggtaaa
      421 cttttgcttt ttcacttatg acctttggct gttgccctgc cgtcttaccc acatcctcgc
      481 ccaggcaggt aaacttttag ccgttagcca tcagctccct gctccctgct ccttgctcct
      541 tgcttcgtcc tccgtattcc gttctccgtt ctccattctc cgttctccat tctccgttct
      601 ccattctccg ttctccgtcc tccatacttt tccctacctt tgtcccgtca actcacggtc
      661 atggccttaa cggtatacag cgatgaatcc ttcatgcgcg aggcgctgaa ggaagcgcac
      721 aaagcgctcg aaaaggacga ggttccgatc ggcgccgtca ttgtatgcgc taaccgcatt
      781 atagcccgcg gccacaacct caccgaacgg ctcaacgacg ttaccgccca tgccgaaatg
      841 caggctttta ccgccgccgc caatttcctg ggcgggaaat atctggagga atgcaccctt
      901 tatgtaaccg tggaaccatg tgtgatgtgc gccggggcct cgttctggac gcagatcggc
      961 cgtatcgtat atggcgccgg cgacgataaa cgcggttact cgctcgtgtc atccgccatt
     1021 ctgcatccga ggaccagcgt aacacaggga gtgctggccg aagaatgctc ggccctgatg
     1081 aaggacttct tcagaaaaaa acgctgacag tcctctgttt cagcccgcat cccgggcctt
     1141 tttcacatta atccgattac ttttgcatca aatacaggtt acatggattt taagaccatc
     1201 aataacgaca tcacccgtat cacagccgaa atttcggaaa cggcttccta tctctggcag
     1261 aaaggctggg ctgaacgaaa tgcaggaaac atatcggtga acatcaccga atttatcaca
     1321 cacgatatgc gggaattgtt tacgcaaggc aaatacccgt tccagccgct tgaagcgcct
     1381 cacccaggcc tcgccgggta ttcatttttt gtgacgggaa caggcaaacg catgcgcgac
     1441 gttgccaacc atttcgacag gaatgtgctg ctgattgtta tttcggatga cgggaagggc
     1501 tatttcacgg taaaagccaa ctccgagcag accggactga cccccacttc ggaacttcct
     1561 tcgcatcttt ctatccacgg gcaactggta aaagaaggcc ggcgcgaacg ggcggttgtt
     1621 catacacacc ccaacgaact aatatctctt acccacattc cccggttctg caaccaggac
     1681 tctttgaaca acctgttgtg gggcatgcat cccgaaacta tggtcgtagt gcccgaaggc
     1741 gccggatttg tcccttacct gctcaccggc tcgctggaaa tcgccgaagc aaccctgcaa
     1801 aaattcaagc atcaccaggt tgtcgtctgg gaaaaacatg gttgttttgc cattggccgc
     1861 gatgttttcg aagcctttga ccttatcgat accatcgcca aatcggcctg catttacttc
     1921 cagtgccgcg cctcaggcca tgaaccggaa ggaataaaac cggagcacct tgccgacctc
     1981 agggaactca gcaggaaatt ccggtcctga acatcataaa tatcatactc cggtaaagtt
     2041 tatatccttg gttatatcct gaaatgaaac agaaacacat cctgtcggac ggaaagagaa
     2101 ccatcatctg gaaaggtgaa cagtgtatgc acaaagcctt atgcctgaga ggcattcttg
     2161 aagatatgga tataaaggag aataccatcc atagtatctc ttcggcacag gtcagttacc
     2221 ttcttcaccg gattgagatc tgcccttcgg gagccatagt taatgctgaa tcggcggaat
     2281 aaaattttta aggtttttgt aaaaacaccc tgaattttat tattattgta ctctttaagc
     2341 atatttcccc catgggagac attaaaatat tctgcccggt ttgcggctgg gaacctgatg
     2401 gcggcgcata ctggcaatgt tcatactgcc atacggtatg gaataccttc gacacggctg
     2461 cccgttgccc gggctgcgga ttccagtata aatacaccca atgtattgaa cacatgggcg
     2521 gctgccctgc ggaaagcctc cacatcgact ggtatcatgg attggataag atcgttgaag
     2581 atgctcttaa aacggaacct gtttccattg aaaggaaaga acttacacct gtcaataaat
     2641 aattcccatc atgaaaaaac tgaccctgat cattgcgtta ctggctctgg caacccttgc
     2701 agacgctcag tggcaatcga acgccgccct gaatactcct atcggcgtat acagcggcga
     2761 acaggttgtg accaaagttg caacccattc ttcgggaatc acatactacg cctggttttc
     2821 gctcgaaggc ggcaactata acgtccggct gcagaaaacc gatgtatatg gcaatatcgc
     2881 tttcggcagc gccggactcc tggtcagcaa ccatacctcc gactcctgga taaccgattt
     2941 tgatatgacg gtggacaacg atacctgcgc cgtcatcgct tttcaggata tccggaccgg
     3001 aaataacgat gtgtttgcct atcgtgtttc accttccggt gaaatgctgt ggggaaacga
     3061 cggcatcagg ctttccacat ccacggatgc cgattatacg ccggtagtca ccgttaccga
     3121 tgaaggaaat gccgtggtag tgtgggagga gtcatcctcg gttgaaaaag tgcgcgtgca
     3181 gaaaatcagc ccgtcgggtt ccaaattgtg gggatcgtcc ggcatcaccc tgcagggcgc
     3241 cggcaccgag agctacacat ttccccgtgt tttcgccgct cccgacgacc aggcctttgt
     3301 gctttggttc aagaaggatg ccggattata ttctcccaaa catctgtatg cccaaaaaat
     3361 ttcccccgac ggcagtccgg tgtggtcatc cgatgtggtt atctttgatg gttccggcgt
     3421 atctatgatc cctaccatcg attttgtccc cgacggttcg aacggcttct acgtggcctg
     3481 gtacgacgac cgcaacaacg actggaatta cagcacttac gtgcaacaca tcgacggatt
     3541 cgggcaggta caaatgacgg ttaacggggt ggaagccatc gtcaacaatt caaacaacca
     3601 catgtaccct tccatcacgc tgtcgcccga cggccaggat ctatttgtct tcgtttcgga
     3661 aatggatgcg gaccaggaaa tgaggggact ttacggacag cggatctcat cgtcggggca
     3721 aaggttgtgg ccgaatacgg gaaaaatgtt catcggcctc tccagcctgg atgtcgggat
     3781 gctcaaggca cagatggccg ataccaatat cgtggtgatt tactgcaaaa acaacagttt
     3841 cggcggcgac caggtaaagg ccatgaagat tgatccgacc ggtgcatacc tgtggccgtc
     3901 gggcgggcat attgtcgttt cctcctccaa cggttcgaaa agcgacctgg tggcaggggt
     3961 ttacaattcc gggcagttta tcgccgcctg gagcgaccag cgcagcgatg ccggcgacat
     4021 atacggtcag aatattacga ccgaagggac gctgggcgca cagcagtttg ccctcgtcct
     4081 ttcacccgat accgtcatct ttgaccagag ctgtagtcct tttatggtcg gaaaatactt
     4141 ctccatcaaa aacccgatgt cttatcccgt ttccatcgac accttagacc tcgatgcttt
     4201 tcttcctgaa ggccagcact ggtacgtgga tactgttctg agtttcccgg tggtaatacc
     4261 gccggccgat tcgctccggc tcaacgtaaa actggattgg attatcaaag atgatttcgg
     4321 aggttatgtg acggatacca tgaatatctt ttcctccgtc gggccctacc gggagatcat
     4381 catgatcgat tcggcgtatc tgtggagcgc cattgataag gctgaaaaat caaaccctct
     4441 cactctttat cctaacccgg cgaccgacct tgtaaggata cggtttaccg ggaacgatcc
     4501 ggttgaatct gtcgtaatta ccgacccgtc gggccgggtg gtaaaacagg ctgccgtgcc
     4561 ggcttctccg gcgccgacgg ttcagttcag cgttgccggt ctgccgtccg gagaatacct
     4621 ggtaagggtt tacacccaaa gagggaagcc ggaaaccggg aaactgatcg tcaggcatta
     4681 atgcgacttt tgttctgaat atccttctgc ggtgaaattc cggaagataa acccggaaat
     4741 cccccgctgg aatcaaatcc aactttctga ttactcatta aaaaaccggc tttacgcaca
     4801 cagccaggcc ggggttatgt tcaaccggga accgaggacg aaggacgaac tcagcgtaag
     4861 ctaattatcg ccggattccc gctccgggac aggcttttca gaccgaagat aaccctattt
     4921 actggcaatc gatgttttta ataaatgttt ttttgccggc ttttgagtct taataacaac
     4981 cgtttccgct atcttgtcgt gaagagtcat tctgtttggg ttccagaata tctgaataaa
     5041 accaagtcct aattcaagag tggatgctat atatccaaga ctacgctcaa cgcagagcca
     5101 taaattaagg cgacgatggt aaaaagatac aatttttatc cttaatatct ttttccctat
     5161 gctctgtcca ttcgtaataa aggtcgtcag ggtaaaataa atggttggaa taaacccaaa
     5221 gagtatttta agaagttgaa ctttattctc actgtatttc tttgcttcta tttcaatatg
     5281 aaaaagattt ttcatggtaa tgttatctat tttcagacca agccaatagg ataacctggc
     5341 cagcgcaagg aaaatacagg agattaccac aaaatcaatc agcatggcta ataatctgcc
     5401 tgaaaatgtc gcaagcgtct gttctgaatc taacaaccat tcttgtcgtt ttttatattt
     5461 catactctat tcgattttat tttctaatta cagtaattgc caacggttgg cggttcgtga
     5521 tttatttaat tttcccgaca ctaaatcgta ataccgtctg caatttatcc ggtgaaacct
     5581 ttcttgcatc actaagataa atttctctat gtgtttttgt tttacgttct aatctatcca
     5641 tttcggcaaa attttccata atcttaaaac tcttcggctc atcgtcataa ctaccaatat
     5701 gcagcatttg aatacaatct ccttcaacta tttcctcaaa tctgacttta tctaataatt
     5761 gatgtggttt tttctttttt gtctgttcta gtatctgcaa tgcaaaatcg ttgtcgacaa
     5821 aatcaggttg gcgaatcatt aaattaaaaa ccaaatcgtt cttatttata gttccattaa
     5881 atgattgttt ggctgaatca ttaatatccc aaactccttc aagggggtaa accgtgtaat
     5941 caaaataacc tttgggctct ggtccttttt taggactcat tttaatcccg taggataaag
     6001 aatataaaac cccgatgtat tcagcaaaaa agtcatcatt aggattcccg ttcccttcaa
     6061 ttgtgaaaaa tttgaatttc ggtatattta tcaattcagg tttgttttta ggcagataga
     6121 attgcttctc attcttcttc cattcatgtt tcataatcca tcttttaaac ttgttaaaat
     6181 acaaaggtaa atttgtcaaa tgacgaccct gtgtcagtag tatttgcctt ttttagcatg
     6241 accgccaact aacaaatttc accacaaaca tacccctttt attaattttt ttactctccg
     6301 gaatctggat aaaataaaag agcaaccaga aaaatacgca gaaaattacc gttcactcct
     6361 gagaagccgg ctggtgtcgc cgggcggaac ataactttcg cttaccggat aatcacctcg
     6421 tcggtaaaca ggaacgatgg gtttccggcg gcatggtgcc cttccggcag gttgccccgg
     6481 ctgtgggcaa cgattttcag gtattgcgtg ctcatatttt caaaaccggc tttaaaagta
     6541 tggatgatgg tggtttgccc cgtgggcggc acatcatggg tgagcgtgga aaccagccgg
     6601 tagtccgatc catcgccgga gataaaaatt tgtacatctt tcggcagcaa aatccaggaa
     6661 taggcattct gcaaaaagtc catcgaaacg gaggagaccg gagttgtttt ccccaggtca
     6721 atttcgagtt cgaggtcctg tccctggtaa ccctgccacc ttccgtcggc aaaattcagc
     6781 gagccggtca gcccgtcgag cagcgcctgg tcgccgccgg cggcataagc gggattgtaa
     6841 gtgctgtatg cggctttgat tttcatcgga caaccgatcc ctttatggac cagaacggat
     6901 tgctgcgaga cgacccccag agaattgccc gttttaacgg ccgccttgat gacaaccttt
     6961 gaatcgatca tcagcggacc ggtataaacc tcgctgcgga cggtaggttc ggtgccgtcg
     7021 gtggtataaa caaggatctg gtcgctgaaa agagtggaaa ggatgaccgg acggctgcca
     7081 tcgatcatct ccggctgcgg ttcgatgaac acgaccttat cggcatcgtc gcactgacgg
     7141 gcaagccggt tatagtcgtt caggttttta tcgagccacc agtcgcggtt ttccattttc
     7201 cagagacgga tatactcttt cttgatatca tagagttcgt ttttcagctt ttcgagttcc
     7261 ggctttgcca ggaggacggc gtattcgctt cccgattctt tggcctggta aaggatgatc
     7321 cgggccaggt ttttgtcggc cgaaaaaata acccgtttca gggcaaaaac ggcatggtcg
     7381 agcagatatc ggtttctgcc ggcatcgctt tttaactgct gaatcttctg aatcagggtg
     7441 ttggccctag taaccacttc caggtttttg tcgccgtaat cctcactggt attttccagg
     7501 ttaaaatcga gaagatcctg ccagaaggcg ccttcgccca cgatgcccct cacgggcaga
     7561 aggcgcaggc tgtcgatggc gaaaagagcc ggggtgatgc cttcggcgcc aaaaaataat
     7621 ctatcgaaac tccggttgaa ctgagccagt ttctcatttc gctccctaac ggccagttct
     7681 ccggtggaag gcggcgccgg gcgccaggag cattcggcgc cccaggccag tccgtgccag
     7741 ttgtaattga acaggttgtg gccgttatcg tcccatgctg tgttcatcat gccgatagcg
     7801 ccgagtttgg cgccatcgcg cacataatta ttgatattga tggcggcatt gctcatcccg
     7861 ggccataatt cgctccagca gctcaccccc ggcgccacca tgaaatcgaa accggtattt
     7921 ttaaacggca gaatggcgtc gtcgaagctt tcggcggcat ggtaccccca ggaaaggata
     7981 accaggtcct tcggcagctt tgagatgatc ccgctgttgt tcacggcgat gtcgccccac
     8041 atcatcagcc gtttgccgta aggcttcaat aatttatcga tccggttgat gtgataagca
     8101 tagatgccgt caaggccgat gctgtcggcc atccgcttgc tcctgccctc gcccagcccc
     8161 tgcgtttcat cacagttgat gttgaagaac gggcttttat aggccggcac gatttcggaa
     8221 tacacatcgc ccagaaacct gtaagtatct tcgctggccg ggttaaggat atcgccgttt
     8281 tcgcgcagtc cggaataaaa cgggttggca aggattttcc ccatgtgccc gaacgactga
     8341 aaattgccga tcacatccat gtggtattgc gccgcatagg cggtaagttc tgcgatttct
     8401 tcggcggtga gtgactccgg aggggcaata tccggatact ttttcaggct gaaggtatgt
     8461 tcggtgtaaa gtgtgaagaa gttcagcctg aattccgaca ttttccggat gcagtctttc
     8521 aggaaatcca ccgtaggaat cgggccgcgg ctgatgtcgt ccatccagcc ccggtacctc
     8581 agcgccggcc agtcggtgat ggtgagcgcc ggaatggaat tcccggtccg gtttgcccgg
     8641 atgagctgtt tcagcgtctg cacaccatag aataaccccg cttcggtgcg ggcggcgata
     8701 aatattacat cttcgccggt acagaggata tagccttctt ccgttgcctt atcgggaagc
     8761 gtcaggttcc tttcggccaa cagctcgtcg attttccggt cgcggcccgt caggcccagc
     8821 aagatgccgg cttttttcgg tttcgtcact attttcgcgt caaggcccgg gccatccttc
     8881 agttcctgct gcaactgccc ggccgaaaaa gcggtttcgg catatccggc attgctgata
     8941 taaatgtccg gtttcgcggc caggtcaaag gtcccggttc cggttttgat ttcctgcggc
     9001 tgcggcgaca ggaaaagggg ctgctgtgcc ttcacaatca atggcagcag caaaacctga
     9061 aggacaaaca tcttcaggaa tacaggacga ctgaacatgg ctgatgtttt tatacggcga
     9121 agttagccat tcgccgacac gaaaggaaac acagatgata caaatttggc cggaaatcag
     9181 gtttactctc tttattcctg atttttaaat ccaaaaatta cgaccttcgt gactttcttt
     9241 taaagtgcta ccctacaatg aagaaattac tcattttgtc tgccatcatc cttggtttta
     9301 cgatagtgca ggcgcaatcc gtcaccgatc tgattgccag gtcttcctac cctggcacag
     9361 gcatggttgt ggtgttcgac agcacccgcg tacaggttga agaaagcggc ctgagccacg
     9421 ttttcaccca ccggctttat aaagttcaga cggctgcggg agctaaaagc ttaagtgtga
     9481 tcaaatacga ttacgatccc ctgtcggcct ggagccagat ccgtcgcgct gttatttaca
     9541 aagccgacgg atcgcaggta gtcctcgaca cggctaaggt catggattat ccccagccgg
     9601 cacgcgccat ttactggggc gcccgcgaaa agatgcttgc cgtcggcaaa ctcgagcccg
     9661 gcgatgccct ggaagtattc ctctatcgca aaggatttac ctatgccctg ctgagcgacg
     9721 gttccggcga cgacgaccgt tacgctcccc cgatgcgcgg ccattactac gatatcgttc
     9781 ctttttacag caacgaccct atcctggtca agagtttcga ggttagcatt ccccggacca
     9841 agacgttaca atacgaagtt tataacggcg aggtgacttc caaagcatgg attgccggcg
     9901 accgcatggt ttataccttc tcctgtaagg acatcaggcc gtggaaaagc gagccgaggg
     9961 ccgtggatgc gtcggacatt gccccgaaac tgttgctgag caccagtcct gactggaaag
    10021 ccaagagcct ctggttttat tcggtgaacg aagaatacgg cagttttaaa tccactcccg
    10081 agattcagca gaaagtaaac gaactattgc gcaacgcgcg caccgagttc gattcggtaa
    10141 gcattctcac ccattgggtt gccgacgaga tccgttatct cggcctgacc atgggcaagg
    10201 gagaaggtta taccctgcat aaaggcgaga tggattttgc cgaccgatgc ggcgtttgca
    10261 aggacaaagc cggaaccctg atcaccatgc tgcgggctgc aggtttcgag gcctatcctg
    10321 ccatgacaat ggccggatca cgcatcgaat atatcccggc cgaccagttc aatcattgcg
    10381 ttacggtggt gaaaagccgc gacggccagt accatcttct tgacccgacc tgggtgccgt
    10441 tcctccgcga agtgtggtcg agcgccgaac agcagcagaa ctatctgatg ggcgttcccg
    10501 aaggcgccga tttggccgaa accgctcttt ccgacccggg caaacattac ctgcgcattt
    10561 cgggaatgtc ggaaatcctg cccgacggaa ctttgctggg agaatactcc gttactgccg
    10621 aaggtcagat cgatgcttcg atacgcagca tttataccag gggttatatc gccgaccgcg
    10681 ctgccgccat tgaaaaagag atacttcatg tttcgccgca ggcacaactc ctgaaaataa
    10741 attgcgacga ccccaccaat tacatggcag ggcccatcaa catgcatttt tcggtcaaaa
    10801 taccggatta tgcccttgtt acggataacg aagtgatttt cacgccgttc accatagctg
    10861 ggttcttcaa acgggcccag ccgcaggtat atttcgaaac ttctcccgaa aaacgtgaat
    10921 accccttccg cgaccgcacc tcgcacgcgg tcagcatctc ggaaaaaatc agtcttcccg
    10981 gaacggctac accggtttcg cttccttcaa atgaaaccga taccggcccc ggggctactt
    11041 accggggtga atatgtgatg gataacggga aattatcggt gaattttacg gcccagttcc
    11101 cgaaacggat ctaccaaccc gaagactggc catcgtttcg cgaagccgtt gaataccaga
    11161 acaaactggc tgaaacgcct gtcaggctga agttgaaata attcccggag gaattcagaa
    11221 ataatttaag atcagaacca tgaaatacca aaatatcatc ccgatcatcg ttaccctggc
    11281 cctggcattg ccggccctgg cacaggaacc cgcctccgat gccgtttacc atcagcaaac
    11341 ccgggagtat acgctcaacc atgacggaag ctggacttat cattacagcc attccctgaa
    11401 aatcaatacc tatctggcat ttcacaacct gtacggtgaa gacttcatcg tttacaatcc
    11461 gaacgcccag aaactgaaaa tcaacaaatc ggtgaccaca acgccggccg gtaaggtagt
    11521 cccttcgccg gacaatgctt ttaacgaact gctgccaggt tcctgcgcag gcgcacccgc
    11581 ctacaatgac cttcgggaaa tggtggttac ccacaccgcc ctggaacgcg gagccgttat
    11641 tgatttcgat tatacacttg ccacacagcc gtcgtactgg ccctggatgg cggcaaacga
    11701 tgtgctgtgc acgaattcgc ccatcgaaaa acttacctat atcgtgcatg ttccggccgg
    11761 cattacgctg aactacagcc agtacaatat cacgcaacaa cccaccgtta ttaaaagcgc
    11821 cggcaaaact acctacacat gggtgctgac tgatattccc gccgccatgc gggaggattt
    11881 ccggtcgaga gagcaactcg gccgcccgcg cattgttttc tccaccgcaa aaagcctgtc
    11941 ggaaaccatt tccggtttta cttcacagct tgcctttacc ttaagcactt ccgacgaact
    12001 gaaaacggtg gccgctaaag tgcgcgatga gaaaaaagag gacctggccg ttgcgctaag
    12061 gcttcaggat atcgttgcca acgaaatcaa ctattaccct gtgccgctgg caaataccgg
    12121 taaagatgtc cgtcttgcca tggaaacatg gcaggaaaac ggcggcaccg aaattgaaaa
    12181 agcggttctg ctggctaccc ttctgaaaaa tgccggtatc acggccgaac cggtagccat
    12241 attccccgaa agctattatg atgaaaaatc agggaacctc ttttgcattg agaaatatct
    12301 tgtgtcagcc gccatcggga aaaacgaaaa catcctgttg tcgccgctgc agaccgactc
    12361 gtatgacgcc ggtcttgcac ttcccgggaa aaagatggtg accttgctca acggtaataa
    12421 gttcagatcc gaaaagataa gttcgccgct cccggccata tcattaaccg gcaacctttc
    12481 gctggatgat tccatgaaac ttacgggtaa tgttgacctg caactcaccg gaaagctgaa
    12541 tccttacttc accctttccg ccgattcgtc gcgggtgaaa aaattactca acggggcatt
    12601 cggcgaaaaa acggtggcaa aatatacggc gggtgaactc accgccaaaa aaacaggcgt
    12661 tcaatacacc attgaaaaca aagacgggct gaaagaagtc tccgggctat attttctgag
    12721 gataccggtc cttgcttccg gcgttgattc ctggcacatg accgaacttg tttcacaacg
    12781 ggttgaaccg ctcgaaattc ctttcaggat acgcgagtca tgtcaatacg ccatcgcatt
    12841 gccggcaaat gtgaaagtcc tgacaaagga tgcagacgtc aaccttaaaa atgatatggg
    12901 aagcatcagg atcatgatca aacaaaatgg cagaaacctg gaaataaccc gggaaatagc
    12961 attaaaccaa acgatcgtca atcccagtcg ttacggatca ttccgggatt tcatcaacac
    13021 ctggaataac cggaaatatg ccgaaatagt cctggaaaag aataattaac aaatccctta
    13081 aaaccgggaa gctatgcgca cggccattct cctgtcgatt ttcctatgtt tttggtcaac
    13141 gttactcact gctgccccgg agggcgacct ggtatcgttt acctcttcca acctgcccat
    13201 tgtcatcatc aatacgcacg gactgactat caaggacgat ccgaagataa aagtcgacct
    13261 cggcatcatt gacaatggtc ccggtgtgcg caataacgta tccgatccgc tgacgttcag
    13321 cggttattgc ggtatcgaaa ttcgcggatc gagcagccag tcgttcccga agaaaagtta
    13381 cggcttcgag acctggaacg ccatcatgca gactgtcgac acttcgttga tgggcatgcc
    13441 ttcggagagc gactggatcc tcaacgccaa ttattcggat aagacattat gccggaatac
    13501 gctggcctac caaacctgga tgaatatggg gcattatgcc acccggtaca ggcatgttga
    13561 acttgtgatc aacggagaat atcagggtat ttatattttt tcggaaaaga taaaacgcga
    13621 caaaaaccgg gtggatatct caaaactgaa accggacgat atcaccggcg atcagcttac
    13681 cgggggatat atcatcaaga ttgacaagac aacggggtcg ggcggcggcg gatggacgtc
    13741 aaattatctg cccctggcgc atccttcggg acaaaccatt tactttcagt atgaatatcc
    13801 gaaggacgat gaaattgttg aacagcaaaa gtattatatc cagcaagtta tagatacttt
    13861 tgaaacggtt ctggcctcgc cctatttttc ggataccctt atcgggttcc ggaaatatgc
    13921 ttccgaaaac aaactcatcg attacttcct ggttaacgag ataagcaaga atgttgacgg
    13981 ctaccggctg agcacatatt tttacaaaga ccggaaaagc aagggcggca aactaaagat
    14041 gggccctgtg tgggattacg acatcgcctg gcacaatgcc gattattgcg ggggcgactt
    14101 atattcaggc tgggcgtacc agtttccctg catggacgat tattatcagg ttcctttctg
    14161 gtggggcagg ctcttcgacg atgccctgtt caaagaccgg ctgaaatgcc gctggcaact
    14221 gttgcggcaa acgtacctga gcgaggagta tattgatggc tatatcgatt cgattgcgtc
    14281 aggtcttgac gaagcccagg cgagaaattt catcaaatgg cccattctgg gggtttatgt
    14341 ctggcccaat ccgcaaccga tacccgcgac ctatgccggg gaaattgaga gcctgaaagc
    14401 ctggatcggc aaccgcctca actggatgga agcccacctg cccggaacat gttccacgac
    14461 cgccattgcc ggtgctccgc aagctgaagg aacatttgcc agttacccga acccggtttc
    14521 gggagtgctc accctcgaaa tcgacattcc tgcagcgaca aaggtaaccg gtaccatttc
    14581 aaccattgaa ggccgtgaaa tcatgcgctt caccgacgga ttaagagagg ccggctttag
    14641 ttacgaccag gtgaacatgc agcaactgaa gcccgggatg tacctgctga aggtcatcat
    14701 cgggaatacg gtccatgtga aaaagatagt aaagtcgtaa ccggatcagc gtgttacgat
    14761 acgccccaat tgcttattga tctgggcatt caggattttc aggtccttga acctcctcat
    14821 cagcgacata acatcggtat caggttcagg gtccttgatt tccttgccgg tttcggccag
    14881 cagttgttcg atgcggcggg ctttgaaggc aagcagcgag gaagtcacag cccgttcaag
    14941 cacttcttcg tcttcctcga gagggatatg gatctgtcgt ttgctccagt catgcagctc
    15001 gtagggcgat gccagcaggt cgacggcaca acgggcaata gccggatcgg cgtgttgcgt
    15061 catcatttca ggctttattt ccgtttccga atcgaccaat ttctcaaatt ccctgaacac
    15121 tttcacataa ttttcaaagg tcagctccag gccgtcgttg cgcaagtcgt tgacgatgaa
    15181 ttcggccacg gaggtaaaaa cctcttccgg ttcactttgc ccctcagcca gccggtacat
    15241 ttttatccgg cggaacccgt aagccagcag tagccgggta atttcacgct cctgaaattc
    15301 ggtgctgttg aggtcaaggg tggagggctg ttcggccggt atgacgggag gctcaggaat
    15361 tgccgcctcc atctcttcct tcgtcatttc ggcttctttc ctgaacttat tccggagaac
    15421 cttattcagt tcattcagga ttaccgcttc gtccattttc atgcgctccg cgctttcctt
    15481 cacataggcc atgcgcgtaa ggcggtcggg aatcagcgcg atgctgttga tgaattcctt
    15541 gacgatatgg acttttttaa gcggatcgtt accggtttca tcgagaagca gatcaatttt
    15601 gaagcggata aaatcggtag cggcgctgct gataaattcc gtcacctcgc tgctgcgccg
    15661 cgtgcgggca aacgaatcgg gatcttcgcc gtcgggaaac agcaccactt tcacgttcat
    15721 tccctgttcc aggatcatat caataccgcg gaacgaggct ttaatgcccg ccgggtcgcc
    15781 atcgtaaaga atggtgatgt tgggggtgta ccgcttgata agcttgatct ggtcgaccgt
    15841 gagcgacgtt cccgacgaag ccaccacatt ctgtatcccg gcctggtgca tggagatcac
    15901 atcggtatat ccttccacca gaaaacacat gtcgttggcg gcgatggcat tcttggcgaa
    15961 agcgatgccg taaagcacct tgctcttgtt gtagatttcg ctctccggcg aattgacgta
    16021 cttggctttg gatttatcgg aactgaggat tcgtcccccg aaaccgatca cgcgtccggc
    16081 ctggctgtga atggggaata tgacgcggct gcggaaacgg tcgtataatt tgtcttcctt
    16141 agcaatagac aggcccgatt tatcgagcat ttcccggctg taaccgttca gcaacgcctg
    16201 tttggtcagg ctgtcccatt catcggggca gtatcccaac tggaattttg cgatggtatc
    16261 gtcgctgaat ccgcggctgc ggaaatagga catccccacc gacttgccct cgtcggtgcc
    16321 ggtgagttgt ccggagaaaa atttctgagc aaattccgag acggcaaaca atccttcccg
    16381 ttccgatttg gcggcaatat cttcggccga ctcctcctct tcccggatat cgatatggta
    16441 cttctttgcc aggtaccgta aggcttccgg gtaggtaaat ttttcatgtt ccatcatgaa
    16501 attcaccgcg ttgccggctt tgccgcaccc gaaacattta taaatgcctt ttaccggcga
    16561 taccgaaaac gaaggagtac gctcgctgtg aaaaggacat aacccgatga ggttgcttcc
    16621 gcgccttttc agcgaaacga aatcactgac tacctcctcg atgcggacgg catccatgat
    16681 caggtctatg gtctcgtggg gtatcatgag cgtattaaag gtctgctaag tgttttgtct
    16741 ccccggaatg gaaatacttc tgaaaagcaa aggtaaagat cacagccgga cttttttcat
    16801 tgttttagcg ccttcctcag gcagcaagat tatgtgacca gctaatttct ctcaacagtg
    16861 ttgagagttt tggagagtct ttgtaggtct cataaaattg cttcatccgc caaaggttct
    16921 tgtcagaaaa accttttatt tcaggttcat tttgccggat atattttgct aattcggtca
    16981 ccacggattc accccactca gattgttcaa tttttttgct gacatattca cctattatcc
    17041 aatacaggtt tatcaattca gcattaacgg ctcttatggc attagcccgc gattgtttta
    17101 tcagttgaat gatgtcaata aaccgagtgt tcattttaat tatcgcctgc aacaaattta
    17161 aaaaatattg accttgtttt atccttgcag tcacatatca ttcggctgag tataccggta
    17221 cttccttttt tgcgctggaa tattcattca atgatgctaa tcgtaaaaaa aaattccccc
    17281 gccatcgaaa agcggcccgt tgtaaagttc aaccacctga agaccggctt gcagggtgaa
    17341 ctgcctgacc ttttcacctt cggcatagcg gtttaaactt ccggtaacga acagcttatc
    17401 ctgccacact ccgcaacagc ccccgaaaaa accgttcggg aaaccgggaa gaatgatccc
    17461 cttgggtgaa acataaagcg cttccaggtt gtggttcctg agcacctttt cgattccccg
    17521 gtcggatgtg ataaaatgtt gttcgctgag cggaatgagg ttgcagcggg tgtatgcctg
    17581 tttgaaagtc aaaggcagaa tgtcaaacgt caaaagcaat ttcctgagct ttccatcggt
    17641 gacggaagca ttatgaatca ggaatttttc agtgacgaca gcgttgtaat gtgccgtccc
    17701 gggatagcgg ttgccgaccg gttgttcgcc ggttgtatat gaaatattct tcgtatccag
    17761 ttgcctgaga aatccggcag gcgtgttggg cgctgccacc agtccgtggg gcgtctggca
    17821 gaaaaagata tcaggatgcc cgctgatggc gtcgtacgtg atgcctgaag ttgaaaattc
    17881 cattaattcg ccgaaggccg aaagcttttg cttcgcctga ccgggaattc gtttatcggc
    17941 aatgatcagc atacgggata aagatcagtc ggttgaagaa acgataaaac ggcattgttt
    18001 ccctgaaagc tctttgtttt ccctgaaaac aaccttcctg aatctggtga gcaacatttt
    18061 cttgttaagg gatttatgcc cgacagccgc attgatttgt cccggcggga cttccgccag
    18121 caggttctca ttgacgccgg acgagttgcc ggcaagtttc tgcctgaata tcgccgacca
    18181 ttgcgccgtg gccaccagtt cgccgaaggc cacgtgaaaa ggaccggcta caaggctttc
    18241 gccgctcagc aaaccttcgg acgggtgcaa gcctatcctc aggattttca ccccggcttc
    18301 ctcaaagatg ccggcgatga cggccgcact ctcaacggct tcgtccagcg aaagcggcac
    18361 atagctgccg gcatggtaca ttttttccag ttccgtatcg cggatcacca gggttggata
    18421 aatccgggta tcggaagccc ccagcgcaac gattttgcgg gcggttgcca ccgacctggc
    18481 aggggtgtcg cccggcagac cggtcatcat ctgcagacca agcctgaaac cgttctccag
    18541 gatcagttct gatgcttttt ccacatcgta aaccgtatgc ccgcgaccgc tcaggcgcaa
    18601 tacttccccg tcgagcgatt gcgcccctaa ctcgatgttt tgcacggaga attgcttcag
    18661 gagactgagc cgccggtcat cgatatagtc ggggcgggtg gagagacgta tggactttgc
    18721 ttttccggcc ctgacatatt tttcggcgat ttcgaggaat gattgctgca gtgacggttc
    18781 tatcccggta aaattgccgc cgaagaaacc gatttccgtt tcgctgtccg gcgggatggt
    18841 ggcaaggtgt ttctcaatga tgccggccac ctcgtccggt tgcggcatgg ccatagtgcc
    18901 ggaaatattc cgctggttgc agaacacgca gcggttgggg caggcgagtt ccggcacaaa
    18961 aacgggaatg gcgaaatggc ggggcgtcat acccggtaaa attacacatt atggtcattg
    19021 ttacaggatt cgggcgggct tagaaattgg tgccggatgt actaatattg agtttatgtt
    19081 acctgcagat tgatagataa tttaagcgca cgcgtcagga gacgcgcgcg cgagagagaa
    19141 tttatttaga caaaaagtac ttcttattac tgctttgcat attcatattt ctttcaattg
    19201 ggtcgtacca atgttctttt ttcaggctcc attcccatgg ccaatttcga aaactgttta
    19261 accattttct tctgtttctc tgtttataat ctatatagac tggacctata agagtaaatg
    19321 acgattttga tgaacatttt atcagataaa aattattttt taatggtggt tggtcgaagg
    19381 tttggattaa aattcttttc cccaataagt catcattgta caaactgtat tcaaatttat
    19441 ctcgatcatt catgcaaaaa atatttaatt tgtacaatcc atctactgaa agattaacac
    19501 tatctctatt tttcatatat aattctttat actttacatt ttcataatta aataactcaa
    19561 ttacaaggtc tatttcgata tatcgtgaca tattataaaa gaaaattgag cacaaactat
    19621 ccttagtatt gacctcagaa gtcaggacat tagcaatcct aaatgatgaa tcgttatttg
    19681 gaattaaaaa aagtgtgtca taatttctct cataaaatcc tgacctgaat actaattttt
    19741 tatcagaaat ctttgtaccc tcttcaagta ttgagtagaa tctttgttga ttttggaact
    19801 caatgaattc ttgatttctt gaatagaata aacctaatgt ttgagaaaaa ccatcggtta
    19861 cggaaattga aactgcgaca attaataaga atttatttag aagtctcatc acaatctatg
    19921 tattatttta ttgacccttt gcactttgct ggcacaaagt aaaggcattc tatgacaaaa
    19981 agcataaaat tccccttcta ccgaagttat aaaaaaaccg gggagagatt agccccggtg
    20041 caagcagtga tttgaggttt aatataaaga aatcagcctt tggaggcttt tttctctttc
    20101 ttttcagccc gtttttcctt cagcgacttg gtagcctgtt tcttgggctc ctttttctct
    20161 ttatccatcg attttcccat ggcaatttgg ttttatggtt tttgaggtaa ctaaattaca
    20221 atacatttta gtatgatgca aggagaaaaa ttttggcgtt atccggctta cgccgatttt
    20281 accgtcgggc agcgcggcgg gtgaggcgcc ggtcattagc ctgccgtcat acccataacc
    20341 aacccccagg caggtaaact tttagccttt cgctattcgc aatcagtggc atttcccatt
    20401 aacccggtcc gggtcaggca ctttggccct gatctaaatc cagcctttga gcttataata
    20461 ggcaatcgcg aaagcctccc gccccacgtt tacttcatat ttcaggtggt tccagaactg
    20521 gtaacgcact tgtggccggt gacctatggc cgggatattc cggtttcctt tcagttcctt
    20581 atcctcgaat tgcatctggt tttcatcaat ggagctttca aatgtcggcg cgcccgatac
    20641 ataggtaaac cctgcctttt cgaagcaggc tatggcccgg tacatatgct cggggcctgt
    20701 taccaggatg atcggttttg acaatccttt cccctgccat cgttttgaca gattcaatgc
    20761 ctgctgacgg gtattgcgcc ccttgttttc gaaagaaatc cgttcgtcgg gcacgcccct
    20821 gatcatcaac tcttttgcaa acaataccgg cgaatcggcc gaatcggaaa gatcgcccgg
    20881 aatggcgacg acaacctttg attcaggaaa cctcgcggca catccggctg cataataaca
    20941 acgcatcaat ccgttttcgc tgggcatccc tgcgcctccg agcaggataa cagcgacggg
    21001 ttctttcgcc ggctttcccc tggccacggc aagtttatac atggcataga aaggggcggt
    21061 agtgaaagca aagataaggg aaacgaggaa aatggcgccc agggatatca atgttatgcg
    21121 cagccatttt aacaaacggc ctgatttttt gggttttcgc tttttggggg aagtggccaa
    21181 aggctgtctg attttgggtt aaggcctgtt cactagaaaa gggggcgtcc gcaaaacatt
    21241 gcttttattg agggcgcgat cctggatgta aacctcgaaa gcaatcgtat cactctgcag
    21301 ggccataaaa gcgtaataca tttcaaggta catgctgata tcgccgcgta tgtttttgtt
    21361 tttaccttcc ggcgtcagga tcgggatacg ggaattgaag gtgaagttag tatcggctaa
    21421 tgcgacctcc tggaaggagc catccctcca ctcgagaaat ttgaggaagt aatcgtattt
    21481 gtaaggttca accgtgtcgt aatcatacag cccgatatct ccgtctccgt cggtatagct
    21541 gatggtgagc accgccagcg aatcatagcc atcggacccg ctcatcaccc catagctttt
    21601 aaaagtaatt gccggcactt cgggatattc cactttatca atacatccgg ccagggaagc
    21661 tgataaaatg aacaggacaa atagatgtat ctttgaaagc gctttcataa acctttaaat
    21721 agcggaataa aattacaaaa ttattgtatg gacgttcatg caatccgaaa acagatgttt
    21781 gccatcacca acgaccggca atttaacgac gtggcgctgg aaatatttca ttaccagtat
    21841 ggtcaaaacc cggtgtaccg gcaatttgtg gatgggctgg gcgtgaatgt caacgatatc
    21901 cggtcttacg gtcagatccc gtttctgccg gtcggtttct ttaagacgca ccgggttgtt
    21961 tccggttcct tcggggaaga agcggttttt gaaagcagcg gcaccaccgg ttccgccacg
    22021 agccgtcatt atgttgccga tttctcgctg tatgaagaaa gcttcacccg gacctttaac
    22081 cttttttacg gcgacgttgc tcaatacaac atttacgccc tgctcccctc ctatctcgaa
    22141 cggaaaaatt cctccctcgt gtacatggtg gaccggctcc tgtggaaaag taaccgggaa
    22201 tacggcggtt tttatcttca tgacctggaa aagctggctg cagagatcag ggaagccctt
    22261 tcggaaaata aaaaagtgat gttgcttggc gtgacctatg ccctgcttga tctggccgaa
    22321 aaattcccga tgccgctcac cggggctgtt gtggtggaaa ccggcggcat gaaaggccgc
    22381 cgcgaagaga tcacccgcag cgagctgcat gagaagctca tgagagcctt cgcaacggat
    22441 tccattcatt cggaatacgg catgacggaa ctgctttcgc aggcatggtc gaaaggcggc
    22501 ggactgtatg cctgccctcc ctggatgaaa gtgctgatca gcgataccaa cgacccgctg
    22561 tcgctgttgc cggccgggcg cacaggcggc atcaatgtga ttgaccttgc caacctgcac
    22621 agttgttcct tcatcgccac gcaggacctc ggaaaattaa ataaccacgg gcagtttgag
    22681 gtgctcggcc gcttcgacag cagcgatatc agagggtgct cgttattgac ggtataacgg
    22741 agaaacggaa aacggagaac gcagaacgca aaacgcagaa cgcaaaacgc gaaacgcaaa
    22801 cctccactgc caggtaagcc tgccccctta cacacaagct cgcccggaca ggcagacctg
    22861 ccaccttacg cgcagccttc ccgggcaggc aggtctgaaa cgctggaacc ctgaaaccct
    22921 gccccctcac ccacaatcct tgcaggcagg caggtctgaa accctgcctc ctctcccgca
    22981 acctcgccca agcaggcaag acaaaactta tgaacaaaaa gctaaaagct aacggccaaa
    23041 agccaaaagg gtgagagtcg gcgcgcgggg agatgaacat atattccggt aaagcgggga
    23101 gacc
//
LOCUS       DAOPYV010000046        22828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_2274_length_22828_cov_11.921486, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000046 DAOPYV010000000
VERSION     DAOPYV010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22828
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2274_length_22828_cov_11.921486"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..78)
                     /locus_tag="PKI35_07455"
     tRNA            complement(3..78)
                     /locus_tag="PKI35_07455"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:His,seq:gtg)
     gene            249..932
                     /locus_tag="PKI35_07460"
                     /pseudo
     CDS             249..932
                     /locus_tag="PKI35_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
     gene            1058..2272
                     /locus_tag="PKI35_07465"
     CDS             1058..2272
                     /locus_tag="PKI35_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX44256.1"
                     /translation="MKQRYQATGSLSNLAWRRLMKNRLAVASMILIIISCLIALLGYL
                     ITPDKTPFSNEQNLALTTKKPGFRVQMLYVRKNEDAVKHNIFSTMISGRKSDYTAVPV
                     TAYRMTDSTIIVREYEGEGVAGVQSVFSLADVVYALYGKPVYDRETGSFRFTTPEKAV
                     VVVKAGDLKNEILEKNLKSKTFLLGTDRFGRDVLSQLMIGARVSLSVGCISVLISLLI
                     GIFMGAIAGYFAGAVDAVIMWIINVIWSVPTLLLVIVITFTLGKGFWQVFIAVGLTMW
                     VEVARVVRGQFMSLREKEFVEAGKALGFSNFRIIFRHILPNAMAPVIVISAANFASAI
                     LLESGLSFLGIGVQPPMPSWGTMIRENYAYIILDYAYLAILPGIAIMLMVLAFMLLGN
                     ALRDALDVKTIG"
     gene            complement(2262..3797)
                     /locus_tag="PKI35_07470"
     CDS             complement(2262..3797)
                     /locus_tag="PKI35_07470"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNX44257.1"
                     /translation="MNHVTCKSSLLSALLLLPLLLPFRGLSQEMLGIINSNDAGVASV
                     LINPAATVTSRYYLDINLLTLGVFAENNYIYLKDSEYRFSRFLEKNPEYPVHGENDQS
                     TYDYYNTRDKQAYANLRVMGPSAMLVLDRHAFGFTTGFRTVGSFNRLPYDVAKFLYDD
                     FDFKPQQDINYVHPGYFDGHILSWAEIGGNYSYIFKRDGFNQWSAGVNLRYLAGYAGA
                     YSAIDYFDYIVYDEDTLQVNRADGKAGISLPLDYETNDVVRRPLFRGRGAGMDFGVVF
                     ERKTKGYQNWDRLSLCTQQYNPYVYRIGLSILDVGRIRFNKNTYYMEMEKASTFWAGI
                     SSLDYTTVNNYVGEISYHFLGDSSALIKGNQITIGLPTALSLQADYNFRPNWYVNGTV
                     VVPVKVFKNSVSRESLIAITPRFENEKFGISLPVSLFNYYKPRVGIAFRYKGFFIGSE
                     KPSGFFHFSDFTGLDFYTGIKISFAKGDCRRKAQSECGDQQYLKYKNKDKGKPKMGLG
                     ITQ"
     gene            4002..4811
                     /locus_tag="PKI35_07475"
     CDS             4002..4811
                     /locus_tag="PKI35_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44258.1"
                     /translation="MTKTALWWIFAIFFTFAVGEYQKRTGPTYEVRGKTFINNHPFRY
                     KLIRTAENDKDAEISLKIPDTTVQGTIRYKRFKSNDEWSNASLTRDGSKLVFMMPRQP
                     SAGKMMYDIELTSGREKVRLAGKDGEPVVMRFKGHVPRGILVPHIIFMFLGLLFSTRT
                     AIEAMIHGRETFHYSLVTLLFLVIGGLIFGPIVQKYAFGAFWTGWPVGHDLTDNKTVL
                     MVLLWVFAALKLKKDSASRLWPVIAAAGVLAVYLIPHSILGSEIDYTKIPR"
     gene            4815..5435
                     /locus_tag="PKI35_07480"
     CDS             4815..5435
                     /locus_tag="PKI35_07480"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNX44259.1"
                     /translation="MPVTLFILVITILVSILAFSNQGLYRRMAFNPYDIRQFRNGYRF
                     LSYALVHADWVHLLINMLVLYSFGRAVEVYYGMIWGLKGQFYYFLLYAGGIVFSTTPA
                     YAKHKDDYTYTAVGASGAISAVVFASIIFDPLNKIYIFMIPFGIPAVIFGLLYLVYSW
                     YMAKKNVDNIGHDVHFWGAVFGFVFTIALKPSLALHFVDTLKMLLG"
     gene            complement(5443..5994)
                     /locus_tag="PKI35_07485"
     CDS             complement(5443..5994)
                     /locus_tag="PKI35_07485"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNX44260.1"
                     /translation="MLEPSGGKLLISEPSLSDGFFARSVVLLVDHNAEGSFGFVVNKP
                     SGAKLNEITPEFGSSDLKVYLGGPVMMDNLYYLHTRGGDIEGSIPVIPGLWWGGELEI
                     VKEFMRTGRLKPVEIRFYIGYSGWSPKQLDREISEHAWIVHPADLKHIWTRSPDTLWK
                     KLVLSLGKDYAMWINHPADPAFN"
     gene            6160..7560
                     /locus_tag="PKI35_07490"
     CDS             6160..7560
                     /locus_tag="PKI35_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44261.1"
                     /translation="MAKKTSGQQKFEQFRSQYPYFEYKGFDASIQDGSLKVIYDFSLS
                     GSHSFHPTLSIPPRKFYKQPFNAALLSSQVLHSLLFHIGMIELVSYWKAACPERVIIK
                     GHTLDSRQVKWWKKVYFNGLGEFFYTNGINCTQDDFMKLETEGPETETFETELSDSVI
                     VPVGGGKDSVVTLEMLRNDFNIHPFILNPRGATLETAFTAKFGRHEIIEANRTIDPEL
                     IRLNQAGFLNGHTPFSAMLAFYSLLVSWLSGIRHIALSNESSANETTVAGSSINHQYS
                     KSYEFEKDFREYVKTYLSPEFNYFSYLRPLNELQIAGLFADHVRYYPVFKSCNVGSKT
                     DIWCGHCAKCLFAWIILSPFIPLDELIHIFGKNLYEDPSLIGFLKQLTGIDEVKPFEC
                     VGTIDEVNVALMLFIRKYQKGRWPVLPDYYSNSLKYNEYLNFGPETLLKHFNDEHFLL
                     PGFIQILEQRLACKNW"
     gene            7545..8930
                     /gene="murD"
                     /locus_tag="PKI35_07495"
     CDS             7545..8930
                     /gene="murD"
                     /locus_tag="PKI35_07495"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNX44262.1"
                     /translation="MQELVRNRLEGRKIIIAGFGREGRSTYHFLRRIMPLHPLTIADC
                     NMNVAADPEIINDDKAEILTGDNYLENLQSFDIIFKTPGIPTAQLPDNLDRKKITSQT
                     DVFLRLFGRQVIGITGTKGKSTTSSLVHHILKSGGRRTVFVGNIGIPPLDMAEQITPD
                     TIVVMELSSHQLEYLTRSPHISVLLNIFQEHLDHYNGYEDYQQAKFNIGRYQETGDFF
                     LYNSDNDLIVKHLAACKGCKHLAIPFTNSKNRNTGIFYRDSKVILDLNGEEQTVFDDR
                     AGHLLKGQHNLLNIMAAAASCFLAGIDEPSISKAVRTFSGLEHRIEYVGKFGGIEFYN
                     DSIATIPEASIAAVKTLQQVDTLILGGFDRGIDYGVLYPFLAASNVNNLIFIGEAGAR
                     MKNEFEETHLPGKSLYLARDFNEVVEIAFRVTARDSICLLSPAAASYDMFKNFEERGR
                     IFKKLISEYKK"
     gene            complement(9002..9316)
                     /locus_tag="PKI35_07500"
     CDS             complement(9002..9316)
                     /locus_tag="PKI35_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HNX44263.1"
                     /translation="MGKGDKKSRRGKIHIGSFGRRRRPGKTVFTKPEQAAPAVEKVDK
                     IKKAAPVEEIVQPAAAVPEIVEKPVKVKAKKAEAADPAAEKKTVKKAPRKAKETPAES
                     VE"
     gene            complement(9380..10069)
                     /locus_tag="PKI35_07505"
     CDS             complement(9380..10069)
                     /locus_tag="PKI35_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNX44264.1"
                     /translation="MDYEIKKRLAEYFSGFMTPERNSLFNRIIADRTRYITVVLEDIY
                     QPHNASAVLRTCDCFGIQDVHIIENRYEYNLNPEVALGSSNWLSMRRYNSGQNNTAEC
                     IGALKKNGYRVIATTPHTKDTILTDFDVSAGRFALVFGTEKEGLSELAMSLADEFVRI
                     PMYGFTESFNISVSAALSLFHLSEKMRQSGLPWKLTDEEMLDVKLDWMRYSIKNSDFV
                     ERKFFELLEKS"
     gene            complement(10077..10883)
                     /locus_tag="PKI35_07510"
     CDS             complement(10077..10883)
                     /locus_tag="PKI35_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44265.1"
                     /translation="MIGITSFLLVLTLSLPAYAGWILTGRYIDTDGRVLPQRFFIEHN
                     KFKYEMHDFIYIFDFNNQSLILIDISRLTYCKTTLDAYLAYKRDLMTQRLTELMKSIP
                     EGERSAYREEYMHKVADAGKIPPPTTDSVTISDTKIDFRMMNYDTDKYTVSLNGLRVE
                     DVWIAPALKMNDDFSWDRYFDFMAALSLREGDLNLMTSEIYRKLLSTGYPLRRIMYTS
                     IGNNEWQVNKLEEKNIPDYEFYTPDKCKSVTLQQWFAKTAEKTTRYDDYE"
     gene            11036..11725
                     /locus_tag="PKI35_07515"
     CDS             11036..11725
                     /locus_tag="PKI35_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX44266.1"
                     /translation="MAKKTDATEDKIHVVEDALTKTELFIEKNQKVITIVIVSVIAVA
                     LAVFAFRNLYLIPREKESQAQMFQAQRYFEQDSLNKALNGDGNNPGFLDIIDDFGMTK
                     AANLSKYYVGIIYLKQGNFLEAITYLKKFKSSDEFVGSMALGGIGDAYTELGEREKAL
                     DYYLKAANNKDNDLTTPLYLMRAAWTYEDLGKFDKAVELYNRIKTDYYRSNEARDIEK
                     YIERASSQTKK"
     gene            11798..12778
                     /gene="fmt"
                     /locus_tag="PKI35_07520"
     CDS             11798..12778
                     /gene="fmt"
                     /locus_tag="PKI35_07520"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNX44267.1"
                     /translation="MEKQISIIYMGTPLFAVAPLKAILDHGFHVAAVVTSPDKPSGRG
                     LHIHQSPVKEFALLHNLPVLQPISLRDPEFIGKLKSFHATLQIVVAFRMLPEVVWQMP
                     ELGTFNLHASLLPLYRGAAPINWAIINGETETGLTTFMLKHEIDTGDILMRQKFPVFP
                     DDTAGDLHDRLMNASGGLVVKTIEGIRNGSIKPVGQDIQMVQAGDHQSAPKIFKEHCR
                     IDWNATAEKIRNLIRGLSPVPGAFTILESPEGKRRIIKIFGAHIEPTDDLPIPGTLHT
                     DSVKVLKVACKDAWLYLDDLQLEGKKRMKTDELLRGFRIDESWKTVDFTV"
     gene            12959..13231
                     /locus_tag="PKI35_07525"
     CDS             12959..13231
                     /locus_tag="PKI35_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNX44268.1"
                     /translation="MNKAELIEAMAGEAGMTKADAKRALDAFLAATNKALKKGDRVAL
                     VGFGSFSVAKRAARKGRNPQTGKEIKIAAKKVVKFKAGADLVKSVK"
     gene            complement(13359..13958)
                     /locus_tag="PKI35_07530"
     CDS             complement(13359..13958)
                     /locus_tag="PKI35_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNX44269.1"
                     /translation="MILPRDFYTREDVVGIARELLGKKLCTTIGGETAYALISETEAY
                     EGVTDKASHAYGGRRTARTEIMYRQGGTAYVYLCYGIHSLFNIVTNQSGIPHAVLIRG
                     IIPEDNNLALEKRSGMQISEKAGTGPGNVSRLLRIHYRHTGLDLCIPEYQREDGDVIW
                     LEDAGIVIPDKDIIVTTRVGIDYAGEDALLPYRFIWKRR"
     gene            complement(14014..15924)
                     /locus_tag="PKI35_07535"
     CDS             complement(14014..15924)
                     /locus_tag="PKI35_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNX44270.1"
                     /translation="MSENPREILKKYWGYDAFRPMQEEIVASVLEGKDTLALLPTGGG
                     KSVCFQVPVMCREGICIVVTPLVALMIDQVEHLRARNIPAVAVHSGLSRHEIDIAFDQ
                     CVQGNVKFLYVSPERLITRKFSEVARFLNVNLIAVDEAHCISQWGYDFRPPYLRIAEI
                     RPYFPDAPVLALTATAVPVVVKDIQQKLLFKKPNVLQKSFSRPNLAYVVACNEDKFGK
                     LLKVCSSLKGPGIVYVRNRRLTKEISEYLQANNISSTFYHAGLDTATRNRRQQAWMKE
                     EQRVIVATNAFGMGIDKPNVRFVAHFDLPDTLEAYFQEAGRAGRDGKKSYAVLIYNQA
                     DILDARHMFEMAWPEPDLIRKVYQSVGNYFQVAVGSGRDMVFDFDLNDFSSTYKFKPL
                     IVYSALKILEREGLISVDDAFENASRVIFLAGKEDLYRFQVEHEEFDKLIKIILRSFS
                     GVFSGFVPLSEPDIARKLNLQTKDISDRLVKLHQLRILEYIPQRKGTQLVFTCERLAP
                     DDIGFSNEIYFNRKKEARQRLEKVIRYISSPLACRSQALLEYFGEKNAPRCGICDTCI
                     ERVKAEISSEEYRKIETEILKVIRDGACNLKQVVSLVKGIKEEKILKVIRLMADNQVI
                     NADELGNYTLRD"
     gene            16073..16609
                     /locus_tag="PKI35_07540"
     CDS             16073..16609
                     /locus_tag="PKI35_07540"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNX44271.1"
                     /translation="MPYLCTMRVFLIGYMGCGKTTLGKSLSELLNIPFIDLDEDFEAR
                     YRITIADFFLKYGEASFRRIEQNLLHEYMTSDDRVISTGGGTPCFFDNMDQMLKHGIT
                     VYLEMLPAELEQRLAVSIRKRPLLHLKKDEILLDFITQHLSQREVFYRQAHMVVRVPV
                     SNTEALVGEIRRHALFTP"
     gene            complement(16599..18026)
                     /gene="rlmD"
                     /locus_tag="PKI35_07545"
     CDS             complement(16599..18026)
                     /gene="rlmD"
                     /locus_tag="PKI35_07545"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNX44272.1"
                     /translation="MRKKPLPLLEKVTIVDAGAEGKAIARVDDLVVFVPFVVPGDVVD
                     IQVIHRKRNFMEGKVTAFHSYSPDRIEPFCNHFGICGGCKWQNMPYQEQLKYKQKQVV
                     DNFSRIGRFEFPEVMPILASEHTRFYRNKLEYTFTDRRWLTDGDMKMDPEARQMKGLG
                     FHLPQLFDRILDIDYCYLQPEPSNRIRLALKEFALEKKFIFYNARSHKGFLRNIVIRN
                     TTMGGLMVIVVFGLPEFDDEMKCVLDFLAEKFPEITSLMYVLNTKKNDVITDLEVFPY
                     KGDPFLVEEMPDPHGGSNLKFKIGPVSFYQTNPVQAFHLYITAYDFAGFNGSELVYDL
                     YCGTGTISNFIAKSVKKVVGIEYVPSAIDDARENSVFNGIDNTSFYAGDLAKMLNDEF
                     VAANGKPDVVITDPPRSGMHPKVIDQLLAIEAPRIVYVSCNPATQARDIALLNEKYKV
                     EAVQPVDMFPHTHHVENVTLLRIRE"
     gene            complement(18074..19168)
                     /gene="hydE"
                     /locus_tag="PKI35_07550"
     CDS             complement(18074..19168)
                     /gene="hydE"
                     /locus_tag="PKI35_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HNX44273.1"
                     /translation="MPLSVKEILEKESYSGENLVILLDANEADTELIFQHAAEIKRQF
                     VGNKVFFRGLIEFSNICSKNCYYCGIRRGNKKPERYVVAESEVLDAASYALENGFGSM
                     VLQSGERTDKKFISTVETLLRKIKSFSNGRLGITLSMGEQTEETFRRWYEAGAHRYLL
                     RIETSNRELYYKIHPNDARHDYDRRLEALDLLRKTGYNVGSGVMIGLPFQTTSDLAGD
                     LMFFREHDIDMAGMGPYIEHVDTPLYKYKDILLPQQKRFDLSMKMVALLRIIMKDVNI
                     AATTAMQTLDKDGRVKALKAGANIIMPNLTPLKYREDYLLYENKPGTAEQVADSILNL
                     EKQIHAAGCVVGYGEWGDSKHFSRRTSTAG"
     gene            19326..20840
                     /locus_tag="PKI35_07555"
     CDS             19326..20840
                     /locus_tag="PKI35_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNX44274.1"
                     /translation="MLKIFSTEQVRKADAYTIANEPVASIDLMERAAIACYNRIISRI
                     HKETKRVLIYCGPGNNGGDGLAVGRMLWRDGVDVVVVFDAEAEPSPDNRINLERYRQS
                     GGEIASLADRPGIESTDLVIDAIFGSGLTKAITGKWEDIIREINESKAVVVAIDMPSG
                     LFSDKISDSGAGAIIRADHTLSLQFPKLAFMFPESDEFAGDWEVIDIGLHPGYIDSEP
                     ANSFLITAGDIRQIIRPRRKFSHKGSYGHALLVTGSLGKMGAAVLSARACLRSGAGLA
                     TVHIPRSGIEIMQTAVPEAMVSPDLWEDAFTTLPHLENYTAIGAGPGLGKAPKTESAL
                     KLLIQECHVPLILDADALNIISENKTWLGFLPQGTILTPHPKEFERLAGKIVTSFERL
                     DLLQRFSAKNNCYVVLKGAHTVTCTPHGKCYFNPTGNPGMATGGSGDVLTGIITGLAA
                     QGYSTLESCLLGVYLHGLAGDVAAGKNGCEALIAGDIIDYLGEAWLNISAVDQK"
     gene            complement(20847..22709)
                     /locus_tag="PKI35_07560"
     CDS             complement(20847..22709)
                     /locus_tag="PKI35_07560"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNX44275.1"
                     /translation="MDDSAEIYNEDSVKSLKWNEHIRHRPGMYIGKLGDGSSHDDGIY
                     VLLKEIIDNSIDEYMMGYGRSIDVTIKDHLVTVRDYGRGIPLGKLVACVSQINTGAKY
                     DSKVFKKAVGLNGVGSKAVNALSSRFTAQSIRDGRCNSAEFQKGDLVHESGEGASDLR
                     NGTMITFTPDEGLFGNYHYIPEYVEQMLWNYCYLNTGLVINFNNQRLQAKNGLLDMLE
                     RAINGNPLLFPIVHIKDEDFECAFTHSSRQSGEEYYSFVNGQHTTQGGTHLAAFREAI
                     VKTIREFYGKDFEPSDIRSSIIAAVSVKINEPVFESQTKTKLGSQYMEPGGVTINKFI
                     NDILKKKLDNYLHMNSDTAEALYRKILQNEKERKEMANIKKLAKESVKKVSLHNKKLR
                     DCRVHYNTNDERRLESTLFITEGDSASGSITKSRDVNTQAVFSLKGKPLNSFGMTKKV
                     VYENEEFNLLQSALNIEDDLENLRYNYIVIATDADVDGMHIRLLLLTFFLQFFPDLVK
                     SGHVYILQTPLFRVRNKKKTLYCYSDEERLTAIGELGPNPEITRFKGLGEISPDEFSN
                     FIGSNMRLDPVILKHSGDIHKVLAYYMGKNTPERQKFIIRNLRTEDEIEKEMVA"
BASE COUNT         6034 a         5489 c         5261 g         6044 t
ORIGIN      
        1 cagggtgaaa gacgggactc gaacccgcga ctttcagatc cacaatctga cgctctaacc
       61 aactgagcta caatcaccgt gtaaagaggt gcaaaagtaa aaagaatctt aataagtact
      121 acctcccggt gtaatttttt gtttgaaacc accccgctgc catttaatca gagaaataaa
      181 agcggaaaac ccgggattca tgtatggatt taaagctttt caggtagttt tgcattaaaa
      241 aacaggatat gacagacgtg atagattacg ccatatacgg cgacgacatg cagatcgttt
      301 ctgtggaact cgatccaggc gaaggcgtac gcgccgaagc aggcgccatg atgttcatgg
      361 aagatggcat ccaaatggac acgggaaccg gaggcgggat attcaaggga ttcaagcgga
      421 tgatcaccgg cgaaagtttc ttcatcacca ccttttacaa tggagcttca atcaagagac
      481 acgtaacatt tggcgcgccg tatcccggca agatcatccc tctcagcctg agtgaacttg
      541 gcgggacttt cctttgccag aaggattctt tcctctgtgc tgccagggga attgacattg
      601 acgttgcttt tacccgtaaa ataggcgcgg gcctgttcgg gggcgaaggc ttcatcctgc
      661 agcggcttac gggcgacggc ctggcttttg tacatgccgg cggctgcatc atcgaacgca
      721 atctggctgc cggcgaaagg ctgcgcgtgg acacaggctg cctggtcgct tttgcgccca
      781 ctgtaaatta cgatattcag tttatcggtg gcttcacaaa cgctcttttc gggggcgaag
      841 gactatttct ggcaacctta tcgggccccg gcaaagttta cttgcaaagc ctgcctctat
      901 cgcgcctcgc cgaccgcata acagccgctt ctcatggcca cagggaacag tcaagcggcg
      961 tatccggcgt cggcggcaac atcctcggca gcctgctcgg cggcgaccgc tctttttaac
     1021 cagtgacccg gaaacataaa agggacagcc attgtttttg aagcaacgat atcaggcaac
     1081 aggatcactg agtaatctgg catggcggag gctgatgaaa aaccgtcttg ccgttgcatc
     1141 catgattcta atcattattt cctgcctgat cgccctgctt ggatatctta tcacgcccga
     1201 taaaactcct ttttcgaatg aacagaacct cgccctgacc accaaaaaac cgggattcag
     1261 ggttcagatg ctgtacgtaa ggaaaaacga agatgcggta aaacacaaca tcttcagcac
     1321 catgatatcc gggcgtaagt cagactatac cgccgtaccg gtcactgctt accggatgac
     1381 ggacagcacg atcattgtga gagaatatga aggtgaaggc gttgccggtg ttcaatccgt
     1441 attcagcctc gccgacgtag tatatgcact ttacgggaaa ccggtctatg accgggaaac
     1501 cggttcgttt cgattcacta cgcctgagaa agccgtcgtg gttgtaaagg ccggagatct
     1561 gaaaaatgag atactggaga aaaacctgaa atcgaaaacc ttcctcctcg gcaccgaccg
     1621 ttttggccgt gatgtattga gccagctcat gatcggcgcg cgtgtgagtc tgtcagtcgg
     1681 gtgcatttcg gtgctcattt cactgctgat cggcattttc atgggagcga tagccggata
     1741 tttcgcgggc gccgtcgatg cggttattat gtggattatc aacgttatat ggtcggtacc
     1801 gaccctgttg ctggtcattg tgatcacttt taccctcggt aaaggcttct ggcaggtttt
     1861 cattgccgta ggcctgacca tgtgggtgga agttgcccgg gtggtacgcg gacaatttat
     1921 gagtttacgt gaaaaggaat tcgtggaagc aggaaaagcg ctggggttca gtaatttcag
     1981 gatcatattc cggcatatat tgcccaatgc catggctccg gtgattgtta tttcggccgc
     2041 taacttcgct tccgccatcc tgctcgaatc tgggctcagc ttcctcggca tcggcgtaca
     2101 gcctccgatg ccttcgtggg gaaccatgat cagggaaaac tatgcctata tcatcctcga
     2161 ctacgcttat ctggccatcc tgccggggat cgccatcatg ctcatggtac tggctttcat
     2221 gctgctgggc aatgccctgc gcgatgcgct ggatgttaaa actattgggt gatgcctaac
     2281 cccattttag gttttccctt atccttgttc ttatatttta aatactgctg gtcgccgcat
     2341 tcagattgag ccttacgacg gcaatcgccc tttgcaaacg aaattttaat tcccgtatag
     2401 aaatcaagtc cggtaaagtc ggaaaaatga aaaaatcccg acggtttttc actgccgata
     2461 aagaagcctt tatagcggaa agcaattccc accctgggct tatagtagtt aaacaaagaa
     2521 accggaaggc taatcccgaa tttttcattc tcgaaacggg gtgttatggc tatcagcgat
     2581 tcacgggaaa ccgaattttt aaacaccttt acaggaacaa ctaccgttcc gttcacatac
     2641 cagttgggcc tgaaattata gtcagcctga aggctcaggg ccgtcggcag gcctattgtt
     2701 atttggttcc cttttatcag tgctgaggag tcaccgagga aatggtagct tatttctcct
     2761 acatagttat taaccgtagt ataatccagg gagctgatcc cggcccagaa agtggaagcc
     2821 ttttccatct ccatgtaata ggtattttta ttgaagcgga tgcggcccac atccaggatg
     2881 gataatccga tcctgtatac atacggatta tattgttgcg tgcataagga caaccggtcc
     2941 cagttctgat atcctttagt cttgcgttcg aaaacaaccc cgaaatccat tcctgcccct
     3001 cttcccctga ataaaggccg tctcacgaca tcgttggttt catagtcaag aggcagcgaa
     3061 atcccggctt tgccatcggc cctgtttacc tgtaaggtat cctcatcata tactatgtaa
     3121 tcgaaataat cgatagcgga ataggcacct gcataacctg ccaggtaacg caggtttacc
     3181 cctgccgacc actggttaaa gccgtcacgt ttaaatatat aggagtaatt gcctccgatt
     3241 tcggcccatg ataagatatg cccgtcgaaa tacccgggat ggacatagtt tatatcctgt
     3301 tggggtttaa aatcaaaatc atcatacagg aatttggcca catcataggg cagtctatta
     3361 aaagagccca ctgtcctgaa gccggttgtg aagccaaaag catgacggtc gaggacaagc
     3421 atggctgaag gccccatcac ccgcaaattg gcataagcct gtttgtccct ggtattataa
     3481 taatcgtagg ttgattgatc gttttcacca tgaaccggat attccgggtt cttttctaaa
     3541 aaccttgaga acctgtactc gctgtcttta aggtatatat agttattttc ggcaaaaacg
     3601 ccgagtgtca gcaggttaat atcaaggtaa tacctggaag tgacagtggc ggccggattg
     3661 ataagaacgg aagccacgcc tgcatcgttg ctgttgataa ttcccaacat ttcctgcgat
     3721 aatccgcgaa agggcagcaa aagaggcagc aacaacaatg ctgataataa cgatgactta
     3781 caagttacat gattcataga tcgccggaga gtattacaaa ttagttcaat tgctggtgac
     3841 ggttctgtca ttggtctaac ggaaaaacaa tgtttaaatt gcttcatgac cggcattgcg
     3901 ataagctcgc ttttggataa taaatcaaat atttgtaatc ttgcatacct catcggtgat
     3961 cctgaggcct gaataccttt gacacacaac actttcggcg tatgactaag acagctttat
     4021 ggtggatttt tgccattttt tttacttttg cggttggaga gtaccagaaa cggacaggcc
     4081 cgacctatga agttcggggc aagaccttca ttaataacca tccgttcaga tacaaactga
     4141 tccgcaccgc tgaaaatgac aaggatgcgg agataagcct caaaatcccg gatacaactg
     4201 ttcagggaac aatccggtac aaacgcttta aaagcaatga tgaatggagc aatgcttccc
     4261 tgacaaggga cggcagcaaa ctcgttttca tgatgccccg gcagccttcg gcaggaaaaa
     4321 tgatgtatga catcgaattg accagtggac gggagaaggt aaggctggcc ggaaaagacg
     4381 gtgagcctgt tgtgatgagg ttcaaagggc atgtaccccg aggcatcctg gttccgcata
     4441 tcattttcat gttcctggga ttacttttca gtacccggac agccatcgaa gcaatgattc
     4501 acggacgcga aacattccat tattcccttg tcaccctgct tttccttgta atcggaggcc
     4561 tcatctttgg acccattgtg cagaagtatg ctttcggagc cttctggacc ggctggcctg
     4621 tggggcatga ccttaccgac aacaaaacag ttttaatggt tttattatgg gtatttgcgg
     4681 ctctcaaact aaaaaaagac agcgccagcc gtttatggcc tgtaattgca gcagccggtg
     4741 ttcttgcggt ttatcttatt ccccacagca tcctgggatc ggaaatcgac tacactaaaa
     4801 tcccccgata gttcatgccg gttacgctct ttatcctcgt tatcaccata ctggtttcta
     4861 tcctggcctt cagcaaccaa gggctatatc gccgcatggc tttcaatcca tacgacatcc
     4921 ggcaattccg caacgggtat cgctttctct cctatgccct tgttcatgcc gattgggtac
     4981 acctgcttat caatatgctg gttttatatt cattcggcag ggctgttgag gtatactacg
     5041 gaatgatctg ggggctgaaa ggtcagtttt actattttct gctctatgcc ggcggcattg
     5101 tattttcaac cacaccggct tacgcaaagc ataaggacga ttacacttat acggcggtcg
     5161 gcgcttcggg tgctatctcg gcagttgtct tcgccagcat catcttcgat cccctcaata
     5221 aaatctatat tttcatgata ccgttcggta ttccggcagt gatcttcggg ttactctacc
     5281 tggtttactc ctggtatatg gcgaaaaaaa atgtggataa catcgggcac gacgtgcatt
     5341 tctggggagc cgttttcgga ttcgttttta caatcgcact caagccatca ctggctttgc
     5401 atttcgtcga taccttaaaa atgttgcttg gctgaccagc gcttaattaa aagcgggatc
     5461 ggcagggtga ttgatccaca tggcatagtc cttgcccagg gagagaacca gctttttcca
     5521 cagcgtatcc ggcgatcgtg tccagatatg cttcagatct gccggatgga cgatccatgc
     5581 atgttctgag atttcgcggt ccagttgttt cgggctccag cctgaatagc cgatataaaa
     5641 ccgtatctcg acaggtttca gcctgccggt tcgcatgaat tccttcacaa tttccagttc
     5701 tccgccccac caaaggcccg gtattacggg aatgctgcct tcaatatctc cgccgcgggt
     5761 gtgaaggtaa tacaggttat ccatcatcac cggcccgccc aggtaaacct ttaaatctga
     5821 agatccgaat tccggggtaa tttcattaag cttcgcgccg gaaggcttat tgaccacaaa
     5881 gccaaaggag ccttcggcat tatgatcaac cagcaacacg accgacctgg caaaaaagcc
     5941 gtcgctcaac gatggttcgc tgatcagtaa ttttcccccg gacggttcca acatggcgga
     6001 tgacataact atttttctat gatgcaaatt tacgaaaaat aaacccggga tcaggaatgt
     6061 caatattcgc cgtgacggca tttatataaa ggtaaaatag taaatttggg cacggcaacg
     6121 aatgatgatt ccaaagaacc gttgcctgaa tataacccga tggcaaagaa aacttccggc
     6181 caacaaaaat ttgaacaatt tcgctcacag tacccttatt tcgagtacaa aggattcgat
     6241 gcatccatac aggatggtag cctgaaggta atttacgatt tcagcctctc cggttcgcat
     6301 tcgtttcacc caaccctgag cattcctccc cgaaaatttt ataaacagcc ttttaacgct
     6361 gcactgcttt cttctcaggt ccttcactcc ctcctatttc acatcggaat gatagaactg
     6421 gtcagttact ggaaagctgc ctgcccggaa cgtgtcatca ttaaaggaca tacgctcgac
     6481 agccggcagg tgaaatggtg gaaaaaggtt tatttcaacg gcttgggcga atttttctat
     6541 accaatggga tcaactgcac ccaggacgat ttcatgaaac tggagacgga agggcctgaa
     6601 acggagactt tcgaaacaga actttccgat tcggtgatcg tacctgtagg cggcggtaaa
     6661 gattcggtgg taacactgga gatgctgcgt aatgatttca atattcatcc ttttatcctt
     6721 aacccgcggg gcgcaacgct cgaaactgcc tttacggcca agttcggacg gcacgaaatc
     6781 attgaggcaa accggactat cgacccggaa ctgattcgcc tcaaccaggc aggattcctg
     6841 aacgggcaca cccccttttc ggccatgctt gcattctatt cgctgctggt gtcatggctg
     6901 agcggtatca ggcatattgc cttaagtaac gaatcgagcg ccaacgaaac aacggttgca
     6961 ggaagcagca ttaatcatca atactccaaa tcgtacgaat ttgagaaaga tttcagagag
     7021 tatgtaaaaa cctatctttc gcctgaattc aattatttca gttacctccg gcctctgaat
     7081 gaacttcaga ttgcagggct ttttgctgac cacgtccgct attacccggt ttttaaaagt
     7141 tgtaacgtgg gcagtaaaac cgatatttgg tgcggacact gcgcaaaatg cctgttcgca
     7201 tggatcatcc tcagcccttt tattcccctg gatgaactga ttcacatttt cgggaaaaac
     7261 ctttatgaag atccttccct tatcggtttt ctgaaacaac tcaccggaat cgacgaagtg
     7321 aagccgttcg aatgtgtcgg cacgattgac gaagttaacg tagcgctgat gctctttatc
     7381 cgcaaatacc agaaagggag atggccggta ctgcctgatt attacagcaa ctccttaaaa
     7441 tataatgaat acctgaactt cggcccggaa acgctcctga aacactttaa cgatgaacat
     7501 ttcctcttgc ccggttttat tcagatcctc gaacaacgtt tagcatgcaa gaattggtga
     7561 gaaacaggct ggaaggccgt aagattatta tcgcaggatt tggccgcgaa ggcaggtcca
     7621 cttatcattt cctgcgccgg atcatgccgc tgcatccatt gacaatagcc gactgcaaca
     7681 tgaacgtggc tgctgatcct gaaataataa acgacgataa ggcagaaatc ctcaccggcg
     7741 acaactatct tgaaaatctg caatccttcg atataatctt caaaactccg ggcatcccta
     7801 ctgcccagtt gcccgataac cttgaccgga aaaaaataac ttcacaaacc gacgtttttc
     7861 tcaggctttt cggtcgccag gttattggca ttaccgggac caaagggaaa agcaccacgt
     7921 cgagccttgt tcaccatata cttaagtcag gcggacggcg aaccgttttc gtgggaaaca
     7981 tcggcattcc cccgcttgat atggcggaac agattacgcc ggataccatt gtagtgatgg
     8041 aattatcctc gcaccagctt gaatacctga cgcgatcgcc ccacatttcg gttctgctga
     8101 atatctttca ggaacacctc gatcattata acggttatga ggattaccag caggccaaat
     8161 tcaacatcgg gagatatcag gaaacaggtg atttttttct ttacaacagc gataacgatc
     8221 tgatcgttaa acacctggcc gcctgcaaag ggtgtaaaca cctggccatc ccttttacaa
     8281 attccaaaaa ccggaatacc ggcatctttt accgtgacag caaggtgata ttagacctga
     8341 acggagaaga acaaactgtt tttgacgaca gagcaggaca cttgttgaaa gggcagcata
     8401 acctgctgaa tatcatggcg gctgctgcat cctgcttcct ggcagggatt gatgaaccat
     8461 cgatcagcaa ggcggtacga actttcagcg ggcttgaaca ccgcattgaa tatgtcggga
     8521 aattcggagg gattgaattt tacaacgact ccatcgccac tattcccgaa gcaagcatcg
     8581 ctgccgtgaa aacacttcaa caagtcgaca ctttaattct cggcggcttc gatcgcggaa
     8641 tcgattatgg ggttttatat cctttcctcg ccgcctcaaa cgttaataac ctcattttta
     8701 tcggggaggc tggcgccagg atgaaaaatg agtttgaaga aacgcacctc cccggtaaat
     8761 cattatacct ggcccgcgat tttaacgaag tagttgaaat tgctttcagg gtaaccgcca
     8821 gggattccat ttgcctttta tcgcctgctg ccgccagtta cgacatgttc aaaaatttcg
     8881 aagaaagagg cagaattttc aaaaagctca tttcagaata caaaaaataa aaaaaccggg
     8941 atacccggtt tttcataaat acagattccc cggcccttag gcctgacagg gcattagtct
     9001 gttattccac gctttcggca ggagtttctt tggctttcct gggagctttt ttcaccgttt
     9061 tcttttcagc agcaggatca gcagcttcag cctttttggc ttttactttt accggtttct
     9121 cgacgatttc gggaacggct gcagccggtt gtacaatttc ttcaacagga gcagccttct
     9181 taatcttatc cactttttct actgcagggg ctgcctgctc aggtttcgta aagacagttt
     9241 tgccaggacg cctgcgtctg ccgaatgacc cgatatggat tttaccacgg cgtgattttt
     9301 tatctccttt tcccataata ttatgaattt attatcagat tcgtaaaagt aagaaaatag
     9361 ctgaataaaa tcatccagtt taagattttt ccaataattc aaaaaatttc cgctctacaa
     9421 aatcgctgtt ctttattgaa taacgcatcc aatccagctt tacgtccagc atttcctcat
     9481 ctgtcagttt ccagggtaat ccggattgcc gcattttttc agaaagatga aacaaactaa
     9541 gcgctgccga aaccgaaata ttgaagcttt cggtaaaacc gtacatcggt atcctgacaa
     9601 attcgtcggc aaggctcatg gccagttccg acaatccttc cttttcggtg ccgaaaacca
     9661 gggcaaaacg tcctgcgctg acatcaaaat cggtcaggat ggtatctttg gtatggggtg
     9721 ttgtagcgat aaccctgtac ccgttttttt tcaaagcgcc aatacattcg gcggtattgt
     9781 tctgccctga attataacgg cgcatcgaaa gccagttcga cgacccgagt gcaacttccg
     9841 gattcaggtt atattcatag cggttttcga tgatatgcac atcctgtatg ccgaaacaat
     9901 cgcaggtccg caggacggcg cttgcattgt gaggctgata aatatcctcc aaaacaacgg
     9961 tgatgtaacg ggtacggtcg gcaataatcc tgttgaacag gctgtttcgt tcaggcgtca
    10021 taaaacccga aaaatattca gccagccgtt tcttgatttc gtaatccatt tcccggctat
    10081 tcataatcgt cataccgggt agtcttttct gctgtctttg caaaccattg ctgcaaagtc
    10141 accgatttac acttatcggg tgtgtaaaat tcgtaatccg gaatattctt ctcctcaagt
    10201 ttattaacct gccattcgtt attcccgatt gacgtataca tgatcctccg gaggggataa
    10261 ccggtcgaaa gcagtttgcg gtaaatctct gaggtcatca gattcaggtc gccttcgcgc
    10321 agcgataagg ctgccatgaa atcgaaatag cgatcccatg aaaaatcatc attcattttc
    10381 agcgccggcg caatccatac atcttccaca cgcaagccat tgagcgaaac ggtatattta
    10441 tcggtatcgt agttcatcat cctgaaatca attttggtat cggagatagt gactgaatcg
    10501 gtcgttgggg gggggatttt ccccgcgtca gcaactttat gcatgtattc ctcccgataa
    10561 gcgctgcgtt caccttccgg aatgcttttc atcagttcgg tgagcctttg cgtcatcaaa
    10621 tcccgtttat atgcgaggta agcatccagt gtggttttgc agtatgtcag cctgctgata
    10681 tcaatcaaaa tcaggctttg attattaaaa tcgaaaatgt agataaaatc atgcatttcg
    10741 tatttgaact tattatgctc gatgaaaaag cgttgcggca acacccggcc atcagtatca
    10801 atatacctgc ccgtcaggat ccaaccggca taagcgggta aagaaagtgt taaaaccagt
    10861 aaaaatgagg tgataccaat caaaaaccgg gacctggatt tggctgaaga tatattttgg
    10921 agtatttgca tcggggttat tgttaaaaat cggatggaaa gtgttggtaa aaaaacgaat
    10981 gacgttttcc ggattcgaaa aaaaaggcta cttttgcgcc tcaaaattaa gaaatatggc
    11041 taaaaagaca gatgctaccg aggataaaat tcacgtagta gaagacgccc tgactaaaac
    11101 ggaactgttt attgagaaga accagaaggt aataacgata gtaatcgtat cggttatcgc
    11161 tgttgcactg gctgtttttg cattcaggaa cctttacctt atcccccgtg aaaaagaatc
    11221 acaggctcag atgtttcagg ctcagagata ttttgaacag gattcgctca acaaagccct
    11281 caacggagac gggaacaatc ccggtttcct tgatattatt gatgattttg gcatgaccaa
    11341 ggctgctaac ctgtcgaaat attatgtcgg aattatttac cttaagcaag ggaatttttt
    11401 agaggccatc acatatctta agaaatttaa aagcagcgat gagtttgttg gttctatggc
    11461 tcttggcgga ataggcgatg catataccga acttggagaa agggaaaaag ccctggatta
    11521 ttacctgaaa gctgccaaca acaaggacaa tgacctgaca acgccgcttt acctgatgcg
    11581 cgctgcctgg acgtatgaag atctgggtaa atttgacaaa gccgttgaat tgtacaacag
    11641 gataaagact gattattatc gtagtaatga agcgcgcgat attgaaaagt acatagaacg
    11701 ggcttcatcg cagacaaaga aataactgct gattaacttt ttcggaaaat acctggattt
    11761 acattcaggt attttttatt ttagcaaatc tgaaaccatg gagaaacaaa tcagcatcat
    11821 atatatgggt acgccgctgt ttgccgtggc tcccctgaag gctatactgg atcatggctt
    11881 ccatgttgct gccgttgtaa cgtcacccga caaaccatcc gggcgggggt tacacattca
    11941 tcagtccccg gtcaaagagt ttgccctgct gcacaatctt cccgttttgc aacctatcag
    12001 cctcagggac ccggaattta ttggaaagct aaaatccttc catgccacgc tccagattgt
    12061 ggtcgctttt cgtatgctcc cggaagtagt atggcagatg cctgaactcg gcactttcaa
    12121 cctccatgcc tccctcctgc ccctgtaccg cggcgctgca cctatcaact gggccatcat
    12181 caacggcgaa accgaaaccg gactgacgac cttcatgctg aaacacgaaa ttgataccgg
    12241 tgatatcctg atgcgccaga agtttccggt atttcccgat gacactgccg gcgacctgca
    12301 cgataggctt atgaatgcca gtggcggcct ggttgtgaag actattgagg gtatccggaa
    12361 tggaagcatc aaaccggtag gacaggatat acagatggtt caggccgggg atcaccaatc
    12421 agctccgaaa atttttaaag aacattgccg catagattgg aatgctaccg ctgaaaaaat
    12481 ccgcaatctc atccgtggac tttcacctgt tcccggagcc ttcaccattc tggaatcgcc
    12541 cgaaggcaaa cgccggatca ttaaaatatt cggcgcccat atcgagccaa ccgatgacct
    12601 gccgatcccc ggaacactcc ataccgactc agtgaaggtt ttaaaggttg cctgcaaaga
    12661 tgcatggtta tatctggacg atctccagtt ggaaggtaaa aaacggatga agaccgatga
    12721 attgctgcgg gggttcagga ttgacgaaag ctggaaaaca gtcgatttta cagtataaaa
    12781 caacggtcgg cagccttaaa gcgtttaaaa taaagggcat ttattaacaa acttgtcatt
    12841 ttatcaacat tttcaagggg ttcagaccac aaaatgtcga aatcggttga ttttaatata
    12901 tctttgttaa tactaaaagg tttttaccaa tttaaacatc aactaaaacc tctgaaaaat
    12961 gaacaaagca gaattaatcg aagcaatggc cggcgaagcc ggtatgacca aagccgatgc
    13021 taaaagggca ttggatgctt tcctcgctgc aacaaacaaa gccctgaaaa aaggtgacag
    13081 ggttgcattg gttggatttg gctctttctc ggtagcaaaa cgcgctgccc gcaaaggacg
    13141 caatccccag accggaaaag aaattaaaat tgccgctaag aaagttgtaa aattcaaagc
    13201 cggcgccgac ctggtaaaat cggtcaaata actgtgtttg actgaaagca aaagaggcca
    13261 cctttatgaa ggcggcctct tttgcttata gcggttccgg taactgcaaa aacatctgtg
    13321 atataatcaa cttgggggat ttttcttctg cctctatcct atcgcctctt ccagataaac
    13381 ctgtacggca acaacgcatc ctcgccggca taatcaatcc ctacacgtgt tgtaacgatt
    13441 atatccttat cagggatcac aatcccggca tcttcaagcc agataacatc accgtcttcg
    13501 cgctgatatt ccggtataca caagtccagg ccggtatgcc tgtaatgaat tctcagcaac
    13561 ctggaaacat ttccgggacc tgttcctgct ttttcggaga tctgcatccc tgaacgcttc
    13621 tctaaagcca gattattgtc ttcaggtata attcccctta tgagcacggc atggggaata
    13681 ccggattggt tggtaacgat attgaacaag gaatggatgc cataacagag gtaaacataa
    13741 gccgttccgc cctgcctgta catgatttca gtccgggcag tgcgccgccc gccgtatgca
    13801 tgggaagctt tatcggtgac gccttcatag gcctcggttt ccgaaatgag ggcataagct
    13861 gtttcaccgc cgatagtggt acaaagtttc tttcccagca attcgcgggc aatcccgaca
    13921 acatcctcac gggtgtaaaa gtcacgcgga agtatcatgg agcaaaatta tagttaatta
    13981 caagaggctg tttccaattc tcattcgttg aaatcaatca cggagtgtgt aattcccgag
    14041 ctcatcggca tttatgacct ggttgtcggc catcagccgg atcactttca gtattttttc
    14101 ttcctttatc cctttaacca gggataccac ttgcttaagg ttacaggcac catcccggat
    14161 cactttcaga atctctgttt caattttccg gtattcttca gaagagattt ccgctttaac
    14221 ccgttcaata caggtatcgc aaatgccgca gcggggcgcg ttcttctccc cgaaatattc
    14281 cagtaatgcc tggcttcggc aggctaaagg cgatgaaata tagcggatga ctttttcgag
    14341 gcgctgccgg gcttcttttt tgcggttgaa atatatctca ttcgaaaagc caatatcgtc
    14401 gggcgccaac cgttcgcagg taaaaaccaa ctgggttccc ttacgctggg gtatgtattc
    14461 gagtatgcgc aactgatgca gttttaccag ccggtcggag atatctttcg tttgcaggtt
    14521 cagttttcgg gctatatccg gttcgctaag cggcacaaaa ccggaaaaca cccctgagaa
    14581 cgaacgcagg ataatcttga taagcttatc gaactcctca tgctcaacct ggaaacgata
    14641 caggtcttcc tttcctgcca ggaaaattac cctggaagca ttttcgaacg catcgtcaac
    14701 tgaaatcagc ccttcgcgct cgagtatctt caaggctgaa tacacgatca gtggtttgaa
    14761 tttataggtg ctcgaaaaat cattaagatc gaagtcaaag accatatccc gtccgctgcc
    14821 tacagcaacc tggaagtaat tgccgaccga ctgatacact ttacggatga ggtccggttc
    14881 cggccacgcc atttcgaaca tatgacgggc atcgaggata tcagcctgat tataaataag
    14941 aacagcatac gatttttttc cgtcgcgtcc ggctctgcct gcttcctgaa aataggcttc
    15001 cagcgtatcg ggaaggtcaa aatgggcaac aaaacgcaca ttgggcttat caatccccat
    15061 accgaaagca ttggtagcca cgattaccct ttgttcttcc ttcatccatg cctgctgacg
    15121 ccggttacgg gtcgccgtat caagccctgc atgatagaag gttgatgaga tgttgttagc
    15181 ctgcagatat tccgatattt ctttggtcag ccttcggttt cgcacatata caattcccgg
    15241 acctttgagt gaactgcata ccttcagcag cttcccgaat ttatcctcat tgcaggcgac
    15301 aacataggca agatttggcc gggaaaatga tttctgaaga acattcggct tcttaaaaag
    15361 gagcttttgc tggatatctt tcacaacaac aggcacagcc gttgccgtta gcgcgagtac
    15421 cggcgcatcg ggaaaatacg gtcttatttc ggcaatgcgc agatacggag gcctgaaatc
    15481 gtaaccccac tgcgaaatgc aatgggcctc atccaccgca atcaggttta cgtttaaaaa
    15541 acgggcgacc tctgaaaact tcctggtaat gagcctttcg ggagaaacat acagaaattt
    15601 gacattcccc tgcacgcatt gatcaaaagc aatatcaatt tcgtgccggc ttagcccgga
    15661 atggacagca acagccggta tgttgcgggc gcgcagatgt tcaacctggt caatcatcag
    15721 ggcgaccaaa ggcgtcacca cgatgcaaat accctcgcgg cacataaccg gaacctggaa
    15781 gcaaacagat tttcctcccc cggtaggcaa cagggcaagg gtatcttttc cttcgagaac
    15841 cgaagctaca atttcctcct gcataggccg gaaagcatca tagccccagt attttttaag
    15901 tatctcgcgc ggattttcag acatgatgcg aagttaggaa aaggaaaggt tagtttgcag
    15961 aacaggcttt gaagttatat ctgcctgacc gaaaccggcc gccggaacaa ctcatcgtct
    16021 ctcccccggg caaactgcag gccggaaatt tcagcctcca aactccggaa cgatgcctta
    16081 cctttgcacc atgagagttt ttctgatcgg atacatgggt tgcggcaaga ccacgctggg
    16141 aaaatccctt tcggaattgc tgaatattcc atttattgac ctcgacgaag atttcgaagc
    16201 caggtacagg atcactattg ccgatttttt tctgaaatat ggtgaagcca gttttcgcag
    16261 gattgaacag aatcttctcc atgaatacat gacttctgat gacagggtta tttctaccgg
    16321 cggcggcact ccctgttttt tcgacaacat ggatcagatg ctgaaacacg gaatcacggt
    16381 atatctcgaa atgctccctg cggaactgga acaacggctt gccgtttcca tccggaagcg
    16441 gcctttgctg cacctgaaga aggacgaaat cctgcttgat tttatcacgc agcacctgtc
    16501 acaacgcgaa gtattttaca ggcaagccca catggtggtc agggtgcctg tttcgaatac
    16561 agaagcgctg gtcggagaga tacgccggca tgccctgttc actccctgat tctcagcagg
    16621 gtaacattct caacatgatg ggtatgggga aacatatcaa caggttgaac tgcctcaacc
    16681 ttgtattttt cattcaataa ggcaatatcg cgggcctggg tggcaggatt gcaactcaca
    16741 tatacgattc tcggggcttc aatagcaaga agctggtcaa taacttttgg gtgcatgccc
    16801 gaacgcgggg gatcggttat cactacatcc ggtttcccgt ttgcagcaac aaattcatca
    16861 ttcagcattt tcgccagatc gccggcatag aatgaagtat tatcaatgcc gttgaaaaca
    16921 gaattttcgc gggcgtcgtc aattgccgac ggaacatatt caatacccac taccttttta
    16981 accgatttgg cgataaaatt tgagatggtc cccgtgccgc aatataggtc gtaaaccagt
    17041 tccgaaccat tgaaaccagc gaaatcgtaa gctgttatat aaagatgaaa agcctgtaca
    17101 ggattcgtct ggtaaaagga aacaggccca attttgaatt tgagattgga accgccgtga
    17161 gggtcaggca tctcttcaac aagaaatggg tcgcctttat aaggaaaaac ttccagatcg
    17221 gtaatcacat cgtttttctt ggtgttcagc acatacatca gcgaggtgat ctccgggaat
    17281 ttttcagcca ggaaatccaa tacgcatttc atttcatcat caaattccgg aaggccaaac
    17341 accacaatca ccatcagccc gcccatggtt gtattacgga tcactatgtt gcgtaaaaaa
    17401 cccttgtgcg aacgggcatt atagaatatg aatttttttt ccagggcgaa ttctttcagc
    17461 gccagcctga tacggttcga aggttccggc tgcaggtaac aatagtcaat atccagaatc
    17521 cggtcgaaaa gttgtggcag gtgaaacccc agccctttca tctgcctggc ttccgggtcc
    17581 attttcatat cgccatcggt aagccagcga cggtcggtaa aggtgtactc cagtttattg
    17641 cggtagaaac gcgtatgctc agaagcaaga attggcatca cttcgggaaa ttcaaacctg
    17701 ccgatgcggc tgaaattatc aacgacctgc ttttgcttgt attttaactg ttcctgataa
    17761 ggcatgttct gccatttgca tcccccgcaa attccgaaat ggttgcaaaa aggttctatc
    17821 cggtcaggtg aataggaatg aaaagccgtg acttttcctt ccatgaaatt ccttttcctg
    17881 tgaatgacct gaatatccac aacatcgccg ggaacaacaa agggaacaaa aacgaccagg
    17941 tcgtcgaccc gggctatggc tttgccttcg gcgccggcat caacaatggt tactttttcg
    18001 agtaagggaa gtggtttttt acgcatgcta taggatttga cgcccgcgag atattctttt
    18061 caattgttca aaatcatcct gctgttgaag tccttcttga gaagtgtttc gaatcgcccc
    18121 attccccata gcccacaacg catcctgcgg catgaatctg tttttcgaga ttgagaatgg
    18181 aatcggcaac ctgctctgca gtcccgggct tattttcata cagcaggtaa tcttcacggt
    18241 acttcaacgg cgtaaggtta ggcataataa tgttggctcc ggctttcaat gccttaacac
    18301 gtccgtcttt atcgagggtc tgcatggccg ttgttgcggc aatattcaca tctttcatga
    18361 tgatccgcaa cagggctacc atcttcatcg agagatcaaa acgcttttgc tgtggcaaga
    18421 ggatgtcctt atacttgtat aagggcgtat caacatgttc gatgtaaggg cccatgccgg
    18481 ccatatctat atcgtgttcc ctgaaaaaca tcaggtcgcc ggccaaatct gatgtcgttt
    18541 gaaacggcaa accgatcatt acgccggagc cgacgttata acctgttttc cttagcaaat
    18601 ccagggcttc cagtctgcgg tcatagtcgt ggcgggcatc gttgggatga atcttataat
    18661 agagttcacg gtttgaggtt tcgatacgca acagataacg gtgcgcccct gcttcatacc
    18721 agcggcggaa ggtctcttcc gtttgttccc ccatcgacag ggtaattccc agacgtccgt
    18781 ttgagaagga ttttatcttt ctcagaagcg tttcaaccgt tgagataaac tttttatcgg
    18841 tccgttcgcc cgactgcagc accatcgatc cgaaaccatt ttcaagggca tagctggcag
    18901 catcgagcac ttcggactca gcgaccacat atcgttccgg cttcttgttt ccgcgtctga
    18961 taccgcaata atagcagttc tttgaacaga tattcgaaaa ttcgataagc ccgcgaaaaa
    19021 acaccttgtt accgacaaat tgccttttta tttcggctgc atgctgaaat ataagttccg
    19081 tatccgcttc attggcatcc agaaggataa ccaggttttc tccggaatag gattcctttt
    19141 cgagtatttc cttaaccgat aatggcataa cgctaaattg tgcggcaaag ttaagtttaa
    19201 taaagcaatt ataccgggat gtaactgtgt aaagaggggt tgagggaaat atgcagcttt
    19261 gtccggcaga atttgtttcc tgatcgcttt cgcattttgt atttttggat attcaaaacg
    19321 ggagtatgtt gaagattttt tctacggaac aggttcgtaa agccgatgcc tataccattg
    19381 ccaatgagcc cgttgcctcc atcgacctga tggaaagggc ggctatcgcc tgttacaacc
    19441 ggattattag ccgcattcat aaggaaacca agcgcgtgct gatttattgc ggtccaggaa
    19501 ataacggagg cgacggcctt gccgtgggac gcatgttatg gcgtgatggc gtagatgtag
    19561 ttgtggtttt tgatgctgaa gccgaaccaa gccccgacaa caggattaat ctggaaagat
    19621 acaggcaaag cggcggcgaa attgcatcgc tggccgacag gcccggaatt gaatcaaccg
    19681 acctggtgat tgacgctatc ttcggatcgg gacttacaaa agccataacc ggaaaatggg
    19741 aagatattat ccgcgaaatc aacgaaagca aagctgtcgt ggtagccatt gatatgcctt
    19801 caggattgtt ttccgacaag atttccgatt ccggggctgg agcgattatc cgtgccgatc
    19861 atacgctgag cctccagttc cccaaactgg ccttcatgtt tcctgagagt gatgaatttg
    19921 caggagactg ggaggtgatc gacatcggac tgcatcccgg atacattgat tccgaacctg
    19981 caaacagctt tttaataact gccggcgata taaggcagat cataagaccc cgacgtaaat
    20041 tttcgcacaa agggtcgtac ggacacgcct tgctggttac aggcagcctg ggaaagatgg
    20101 gggccgctgt gctttcagcg cgtgcctgcc tgcgttcggg agcaggcctg gcaacagtcc
    20161 atattccgcg tagcgggatc gaaattatgc agactgccgt gcctgaagcc atggtttcgc
    20221 cggatctgtg ggaagatgca tttaccaccc tcccccatct tgaaaattat acagcaatcg
    20281 gcgccgggcc agggctcgga aaagcgccca aaacagaaag cgccctgaaa ctgctcatcc
    20341 aggaatgtca tgtgccttta atccttgatg ctgatgcttt aaatattatt tccgaaaaca
    20401 aaacatggct gggatttctt ccacaaggca caatccttac gccgcatccc aaagaatttg
    20461 aacggctggc cggtaaaatc gtcaccagtt tcgagaggct ggatctgttg caaaggttct
    20521 cggccaagaa caactgttac gtggtcttaa aaggagccca taccgtgaca tgtacccctc
    20581 atggaaaatg ttatttcaac ccgaccggaa accctggaat ggctacaggc gggagcgggg
    20641 atgtgctgac aggcatcatc accggtttag cggctcaggg ttactccacg ttggaatcat
    20701 gcctgctggg cgtgtatctt cacgggctgg ccggcgatgt ggcagccgga aagaatggct
    20761 gtgaagcgct gatagcgggt gatataatag attacctggg agaagcgtgg ctgaatatat
    20821 cggcggttga tcagaaataa ctgacgttaa gcaaccattt ctttctcaat ctcgtcctcc
    20881 gtacgaaggt tgcggatgat gaacttctgg cgttcgggag tgttctttcc catataatat
    20941 gcgagtacct tgtggatatc gccagaatgc ttcagaatca caggatcgag acgcatgttc
    21001 gaaccgatga aattgctgaa ctcatccggc gatatttctc ccagcccttt aaaacgggtt
    21061 atttccgggt tggggccaag ttcgccaatt gccgtcaaac gttcttcatc tgaataacaa
    21121 tacaaggttt ttttcttgtt cctgacgcgg aacaatggcg tctggagaat atatacatgg
    21181 cccgatttta ccaggtccgg aaaaaactga aggaagaaag taagcagtaa taaacggata
    21241 tgcattccat cgacgtcggc atcggtggca atcacgatgt agttatacct gaggttttca
    21301 aggtcgtctt ctatatttaa cgccgattgc agcaggttga attcctcatt ttcgtataca
    21361 accttctttg tcatgccaaa actattcaaa ggcttgcctt taagactgaa tactgcctgg
    21421 gtattcacat cccggctttt ggtgatggaa ccgctggccg aatccccttc ggtgatgaac
    21481 agcgtggatt ccagacggcg ctcatcattc gtattatagt gaaccctgca atctcttaat
    21541 tttttattgt gaaggcttac ctttttaacg ctttctttgg ccagcttttt gatattggcc
    21601 atctccttgc gctctttctc gttctgcagg attttacgat acaaggcttc ggccgtatcg
    21661 ctgttcatgt gaaggtagtt atcgagtttc tttttaagga tgtcgttgat aaatttgttg
    21721 atagtcacgc ctcccggctc catgtactgc gaaccgagtt tggttttggt ctgtgattca
    21781 aataccggtt cgtttatttt aacgctaacg gccgcaatga tggatgaacg tatgtcgctg
    21841 ggttcaaaat ctttgccgta aaattcacgg atcgtcttta caatcgcttc acggaatgca
    21901 gcaaggtgcg tcccgccctg ggtggtgtgc tggccattga caaatgagta atattcctct
    21961 cccgactggc ggctgctatg cgtaaaagca cattcgaaat cctcgtcttt gatgtgtacg
    22021 atggggaaca acaatggatt gccattgatc gccctctcca gcatatcgag aagcccgttt
    22081 tttgcctgca gacgctggtt attaaaattg attaccaggc cggtattgag gtaacaataa
    22141 ttccagagca tttgctccac atactccggt atataatgat agttcccgaa aaggccttcg
    22201 tcgggggtaa aggttatcat ggtaccattt cgaaggtcac tggctccctc gcctgattca
    22261 tgaacgaggt cccctttctg aaactcggcc gaattgcaac gtccatcacg gatgctctgg
    22321 gcggtaaagc ggctcgaaag tgcattcacc gcttttgaac ctacgccatt cagtccgacg
    22381 gcttttttaa aaactttgga atcgtatttt gccccggtat ttatctgcga aacacaggca
    22441 accagtttcc cgagaggaat gccccggccg taatcccgaa ccgtcaccag atggtcctta
    22501 atggtaacat ctattgaacg tccgtacccc atcatgtatt catcaatgga gttgtcgata
    22561 atttctttca gcaggacata aataccatca tcatgcgatg aaccgtcgcc taatttaccg
    22621 atatacattc ccggacgatg ccgtatatgc tcgttccatt tcagcgattt aacgctgtcc
    22681 tcattatata tttctgcact atcatccatt gtggacaaaa ataacagatt aggttcgaat
    22741 ttttacatcg gtcgcgaacg agttttcacc cattttattc acaggctgaa agagaagagg
    22801 caaagggcat ggggctggga gaacggag
//
LOCUS       DAOPYV010000047        22721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_2298_length_22721_cov_12.269964, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000047 DAOPYV010000000
VERSION     DAOPYV010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22721
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2298_length_22721_cov_12.269964"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PKI35_07565"
     CDS             <1..923
                     /locus_tag="PKI35_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44276.1"
                     /translation="MPPTGWTIFGNTSNWVISQTNNAGGALPEGRLKNSPAFTGYMRL
                     ISPLIDLTGQTSVIIQFKHMFEGANFILGVDTRSNYGAWNNVWTLTPTASISGSTVII
                     PVSNADIGSNKFQLALYINGSSANMLNWYFDDIQILSPMPVDAMMAGCDIPNSFMHAK
                     EVTGKVANLGVAAITSMDVNWQINDGAIHTTHYDGLNLGTGEQYSYFCNDSINEAPGT
                     YTFKVWVSGVNGSTGPDGNPDNDLITKDITIHEYFALRRPMFEEFTSSTCSPCANFNA
                     TALNPFIEAHGNELTLVKYQMNWPGSGDIY"
     gene            939..2024
                     /locus_tag="PKI35_07570"
     CDS             939..2024
                     /locus_tag="PKI35_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44277.1"
                     /translation="MRRTYYGVSYVPDLFIDGTRVNTDATGVTNGFNNSIAKQSLVAI
                     NSTHVVDGNNVIIHAIISPYDTYQGVKAHIAVVERKTTGNVGSNGETEFHNVMMKMVP
                     DANGTSVNLLPNQPVELNYTQDMTATNVEEMTDLRVAIFVQKSNKEVFESNYSIETGS
                     FISITPANGATNISLTDPLLIQFSHPVQMKDGSELTNSNAASVIMLKENNINGPNASY
                     TVSVNSEKTLITITPSPSLVFNKTYFLKVDTLENKVSGVHTLATTSSFTTRSNVNVPE
                     NPTVGKVRVYPNPANDFVNVISGETGGIQGLELISLYGNTVRQYTDYIKGGSSAKLDV
                     SEVPAGVYFLKVLGDNKQQMIRLVISK"
     gene            2237..3781
                     /locus_tag="PKI35_07575"
     CDS             2237..3781
                     /locus_tag="PKI35_07575"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HNX44278.1"
                     /translation="MKSKTGIIAVNFSAILFTLLFYKQATGVNLLIFEILYFCWLAVS
                     RQVDFRNRNMLVFGSLLLLTALFTVITHSVFSYVIHYLALLVFVGILIYPEAKSLISI
                     TGISFLNIGGAQKKFLEHLSEYGIKGSRIASLFKRGMIFLFPVIIILAFIIIYRNSNP
                     WFDGVVTNILKHIGNWINLIFKDFDFMIIVTFLIGLLISNILLLRWNNRKIIEKDSSA
                     ADTLERRKSGKQHHFKPNGLKNEYRAGVFLLSALNILILVMNVMDIYWVWFHFTWNGQ
                     YLKQFVHEGTYLLILSILISIFLILYYFRKNINFYSKNSLLKILSYLWLAQNTILALS
                     VGIRNFWYIYYYALAYRRIGVIIFLVLTLFGLYSVFMKVLRRKSSFYLFRVNALAIYL
                     VLVISSAINWDTLIARYNFSHADRSFLHLDYVASLSDEALPYLDHPLAELKRIDTVQK
                     KNFPNEKVIYMKPDTYFSIIENRKVFFKKKWESKGIMSWNLPEYLAYKNMFGKPENSL
                     GMLFLP"
     gene            4083..7526
                     /locus_tag="PKI35_07580"
     CDS             4083..7526
                     /locus_tag="PKI35_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNX44279.1"
                     /translation="MKKLVPLVALLLVFHVSLIAGETRHTYTFGNPVVIKTGSYQLIE
                     FDNTLQTGLVGEPTLPYCKVDMLLAPGESATRIEYQWNNEITIPGTFVLFPQQQVRPI
                     SQDPSGIFLKNESLYAADIKYPGNAGGHLVTSFLNGRSLALSTFTPVRYNPATGQVSY
                     FESVTVTVYSSSSPESREAMKNFRTGNEIESRVKKLVDNPEAATLYSSDDAAAADDYQ
                     VLVITTSAFSNSFSSLVDHYLMEGMACQIMTTEEINTSVSGNDLQEKIRNYITSEYQQ
                     HGVEHVILGGDDELVPHRGLYCFVQSSSAYEDQNIPADIYYSGLDGNWNTDGDSQWGE
                     PGEDDLEPDISVGRMPFSTLNELNAMLNKTVSYQFTPVLGEMRQVLMAGEHLYDNPNT
                     NGSQYIELLIGDHSDNGYTTSGIPENYDFDKMYDEVESWSGSDLMNHLNQGRPMLNHS
                     GHANETFVMKFNNSDITDANFSQVNGTTHNFTVVYTHGCLCGAFDYNDCIAEKMVTIN
                     NFAAAFVGNSRYGWFNEGQTEGPSAHLHREFMDALFTDHYTRIGRAHMESKCATAPWV
                     TAPGQWEPGALRWCFYDCNLLGDPAMAMWTDNPFNATVTYSQAIPVGITTTSVSVHNN
                     GQPAPDVTCAIVKNGLVVGKGITSSTGTADIIFNPAVTEPGPATLYVSGGNSMPASYT
                     LEFIPSTGAYIVYQSVTVNDATGNQDGIPDYGETVSLSLTVQNNGAEDATNVTVTVTS
                     DDPYVTFTDSTELYPSVPVNSWVTIDNGFTFVVSDSIPDMHNIIFNVTATSGNSWYST
                     FSLTAHAPLLVTGNLSISNESLAQNGRLDPGETATVNIPVSNQGHSDCRNMTASLASD
                     NSSVTIANPSITLGNLQKGGTVTASFQTEVGATIPMYSLVELDFTAGSGNLSVEEQYY
                     QLIGVNVEDFETGDFTKYNWDQGDHPWMITSESTFEGDYSARSGVIADGETSQFSITM
                     EVLASDSISFYSKVSCEDTFDKLSFSVNQQEKDAWSGELPWEHHSYMVEAGMNTFKWS
                     YTKDFHEIAGSDCAWVDYIIFPAFRNNAGVDEKEIPGIDLCLYPNPASGELHIKCSHL
                     KPGDNTLSIIAADGRICYKQSLTGSAETVVNTSSLKSGIYECIISNGSVHQVSRAFII
                     R"
     gene            7829..12682
                     /locus_tag="PKI35_07585"
     CDS             7829..12682
                     /locus_tag="PKI35_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNX44280.1"
                     /translation="MALKSTHTYWALLVTFCLTIFTLSSSAEKVTYQGSWGSPGISLQ
                     KNDAQGVTIGFSIKEFDISSRSFNGETMQEIGLTGNFLPNAEGAPNLPVISRYVAIPQ
                     GSQAAIEIKRQRTETVSNIDMAPSPRIPLDTERGPLQYNKDLSIYSKNALYPQQPVIV
                     SQPKKIRGVDVVLVSITPYQYNPVTKELVVIRDIEVNVNFTGGNGHFGEDRLRSRFWD
                     PILKDNILNSESIQPIDYSARMAYQTSHRETGCEYLIIVPNDPMFSQWADTIKKFRTE
                     QGILTKIVTLQEIGSNTTTAIENYINNAYNNWDIPPAAFLMMADHGTNAQTQIPAPIW
                     DNYCVSDNLYADVDNDDLPEIAHARMTAQTAAHLQTMVTKFIEYETNPPTSADFYNKP
                     ITALGWQTERWFQICSEVVGGFWKNSLNKQPVRINAVYQGNPNSDPWSTATNTATVVN
                     YFGPNGTGYIPATPQELGGFSGGTPSQVNDALNAGSFMLQHRDHGYEQGWGEPGYTSS
                     NIPGLTNVGKLSYIMSVNCLTGKYNYSSEVFAEKFHRYTYNNQNAGAVGILAASEVSY
                     SFVNDVFVWGMYDNMWTNFLPDYGALFEERDLIPAFGMCAGKIFLQQSSWPYNTSNKE
                     VTYNLFHCHGDAFLDVYSEVPQQLTVSHNEVMLAGLSTFDVTANEGAFIALSANGEII
                     GTGVGTGSPVAIPVNTPAIGTVVKIVVTKQNYFRHTSSVIVISPENPYVVSTAVAIND
                     PAGNNNQKLDFGETAFLSVTEENLGNTDSQNTLVTLNLTDPNITIVDNTENYGVVPAH
                     QNVTVPSGFQVQVANNVPNGYTATVQATATNGTDTWVSNLALTCYAPALSVAGTIDID
                     DSQSGNNNGRLDAGETVDLIINNYNNGGATALNAIASLASSSGWITINSATYNVGDIA
                     PLASPTAVFNVTAHPNCPAGLHIPIDYTLTAGSYTLEHVYDLQAGIIVEDWETGDFTK
                     FSWIAGGNQPWTITNVAPFEGTYSAKSGTINNSQTSQLILNFNVPADDTVSFYYKTSS
                     EASKDKLRFYIDIIEKGQFSGITGWQQAKFPITAGSHTLKWIYNKDASGSAGSDCAWV
                     DFIVLPSAPTTACMAGPDGYSCQGAPFQCQGEAINYNTVAWETSGTGTFSSQTILNPV
                     YTPSAEDIAAGSVTLTMAATGISGTVVDNMLLSMQDPATANAGANGSTCQGASYFHSG
                     ASATNYNLLEWTTSGTGTFDNIHQLNPIYTPSAEDAAAGSVTLTLNAINQGCGTVTSS
                     ITLVVNPLPAPAITGETTVCQETQNVVYSTPYVTNNSYTWTVTGGEMTTGANPNEIAV
                     TWNEAGTGTVSVAETTPFNCSTTAVADVVVNAVPQPEISGNVAPCAGETVTFTTPQVE
                     GISYTWDAGAYNITSGQSTNEIQVYFDIPGTQNVMVTETDNVSLCSKSAVAAVSINIV
                     PGTPEVPQGPDRADLYYVTSTDVTVTAVPYTAVYTWQISPAEAGTVANTDNNATITWS
                     PSYRGNAEVSVKSGNGCGEGAWSPVKSIEVLNSTGIVEPGKNLGVIVTPNPNDGKFNL
                     TIVTPGTSMIGYRIVNASGATLLEAHGLQSNGKYTQQMETHLTPGVYSIIVNTVNGQA
                     VERFVVK"
     gene            12858..16322
                     /locus_tag="PKI35_07590"
     CDS             12858..16322
                     /locus_tag="PKI35_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNX44281.1"
                     /translation="MKHPLVLNLLVALFLSIGIKSYGTRTWVSVTSGASRPASLQTKS
                     SVNGCVMIDASISGFYTNPVSINGAEYLVVSLPGSYPSLEAGNPGLPYLNATVNIPAN
                     GRFSTRIIESEYVEYHNMRIAPSRGNLPLTAEAAATPYTFGESYDHDDFYPGNLAGGQ
                     ETFIIRNQRGQSFSINSFQYNPVTRTLRVYTKLSVEIYLSETQGENELSGDDLNISNV
                     REITASVQPAFLNNQTRSDGLPDENGQMLIICPGNFSASLKPFTDWKNATGIATHVVD
                     AGSFKTPEELRDFVRDYYYAAGNLAYLLLVGDAEQIPPYIKDDIISDNYYGYLAGDDH
                     YPEVLVGRFSAENINELNTQIDRTLEYEKGLGNNRNYYSTATGIASEMKMGDDGESDF
                     QHTRNLLSTLKDFTYTDFAELYDGSQGGLDADGYPTSQMAIDQINQGTGLILYTGHGG
                     INTWITAQVSSSVVDMLGNNGSYPFIWSVACETGNFKGHTCLAESWLRATDNNGKPAG
                     AVAALMASGVQTTMPPMEAQDKMVDYLAHCSEQGSPRTFGGLSASGMISMNSAYGKYG
                     YAISDTWILFGDPSLAVRTNAPEPILASHKSYIAEGGVVFNVSANVSGGLATLSQDGE
                     LLGSGTISQGKAQIILQSPASGSSILLTITSFNRTPYTSDILVRNKPSDATAVTPVNH
                     SRLIPVDPVFEWNAGDGAQPDGFRLFLGTNNPPSNIINGFDRKDSSFNPNLHLDYNTT
                     YYWRVESYNANGSSYGVVNEFKTVFQPDEDFETGQFKSTSAWVNEGGSWTIDNRDAFD
                     GDFCGRSGIITDNGYSSLKYSCNVNSCDFVSFWRKTSTEENKDKLCFYIDGQLTGEWS
                     GESEWKHEIFHVTAGFHELEWRYSKDAGNGSGEDAVWLDDIHLPVHGSFEAGTTPLGT
                     VCAGSPFVPEASASNYSSITWQTTGDGTFDDCNLLIPQYSPGPLDLSTGSFLLTMQAD
                     GYNGCSSYTGMVVGNINPTPEIMLPEDTLASAGGSIVLDGSCESGIAYEWIQTGTTLP
                     YITVNESDAADGKISLTLKVTNEYGCSSEQTTTVHFVKPGDKPSFSVYPNPCTDYFTL
                     QPENGLMALNHLYVVDARGTIRWQQNSDLTINGSSTFNLPQLASGLYMVVAESEAGAV
                     TRPILINP"
     gene            16488..18509
                     /locus_tag="PKI35_07595"
     CDS             16488..18509
                     /locus_tag="PKI35_07595"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNX44282.1"
                     /translation="MRIRFYAAICILLSAISVMAQQADPNHGLKHWMTPEEASHRDLI
                     GRGFVETDPPAGIVRNVEEFGPAEGVLVRYPFGIPINLIKEMAEDDTVTTIVGNASNQ
                     ASVTSQYQSNGVNLAHCKFLIAPTDTYWTRDYGPWYVTYGDKQVGIVDFPYNRPRPND
                     DDIPKKLATSLGIEWFGMNVIQTGGNYMTDGMGQSASTTLVWEENPGQSHEQIAQKMH
                     DYLGIENYMVVPDPNGTYIDHIDCWGKFLAPDKILIRKVPPTHPQYTLIENTAAFFAS
                     SISSYGTPYRVFRVNTPNDEPYTNSFILNKKVFVPIMGNSNDNAALLAYQTAMPGYEI
                     LGYLANPSAPWESTDALHCRTHEMADRGMLYIKHMPLLGQVTAQADFEINADIVPFSK
                     QPVYSDSAWVIYKVNSGAWDTLSMVNSAGDHWTASIPGQMEGDTVSYFITAADASGRR
                     QNHPFIGAPDPHVFIIQVFSPPDVTVAPDTLQFETWEDMYHGKKALVRNYSSQPVTIN
                     RINTQGTDPFGWYIDPWNIEPPFILNSGDSLILNVKIDLQAGKTDIRCDTLQVSTDAS
                     VHNVFLCLDLDLLTGINPGDGTSSEPEVFPNPVQTTATVGFSIEKAGRVKIDVISGTG
                     QVAATIADNNFSKGKHSLTWNAQKLPSGIYLVRFDDGHTVGYTKVLVNR"
     gene            18723..20051
                     /locus_tag="PKI35_07600"
     CDS             18723..20051
                     /locus_tag="PKI35_07600"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX44283.1"
                     /translation="MLKRKDVQAIKKGDKKLVNGWVMYDWANSVYQLTIASAIFPVYY
                     NSVTRTDGDFVVSFFGAPVINTVLYSWAIAAAYLIVAMGSPLLSSMADYTGRRKGFMR
                     AFTMIGAVACGALFFFNKNTIELGIIAFALGTVGYGGSIVFYNSFLPVIAEPKDQDRI
                     SARGYSMGYLGGVVLLLFNLLMIMKPGWFGISADSDLPARISFITVFLWWIGFSQISF
                     NRLPKYTFRKRMNRAKVLTNGYRELRVVFKQVRKSFQLSVYLIGFFFMMMGLLTTMFM
                     AATFGEKEVGVPGDILILTILIIQLVGMFGAWLFSRISGKIGNLKALVISVVTWILIC
                     VYAYTIKDTAGFMIAAFFIGIVMGGSQSLARSTYSKMLPETTDHTSFFSFYDVMEKLA
                     TVAGTFSFGIIEALTGSMRFSVLAITSFFIISLIFMLMLMRRAKKSEMVI"
     gene            complement(20137..21432)
                     /locus_tag="PKI35_07605"
     CDS             complement(20137..21432)
                     /locus_tag="PKI35_07605"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44284.1"
                     /translation="MKTQQIAFLMLILSGLFAGCRDTFIERRTFTANVPEYLSYDEMR
                     QPVKSSGATAVEVQGKICIRDNYLFVNEKYKGVHIFDNTNPANPQNIATLQIPGNVDI
                     AVTGDYLFADSYVDLVVMDISDLGNPKEVARLKDIFPYTIPEIEDINVNYPIASIDQN
                     KGVITGWKVRTVTEEVEGNINPVYWYDKGLNIYTMEGGNSSGGGGGYATQTIGVGGSL
                     ARFIIYGNQFYGLNQTDMQVVDITAPTKPEIGKKIDLQRSAETVFIDGTHLFIGTQTG
                     MLIYDISSPALPVFISEYNHFQSCDPVVVQDGYAYVTERAGNRCGNWQNLMEVIDIRE
                     ILSPQLVKGYSMTEPWGLGIDNRKLFVCDGAAGLKIYDASDPLRIDENLLKTFTDVTA
                     HDVIPVGNLLILLATDGIYQYDYSDLNNITLISRIPIGG"
     gene            complement(21494..22222)
                     /locus_tag="PKI35_07610"
     CDS             complement(21494..22222)
                     /locus_tag="PKI35_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44285.1"
                     /translation="MKILRIIILIATIIASGLASPAQQVSKDAVYLGNGSILRGRIIE
                     NVVGDYVKIEMVGSNILVIPEKEIDHIIFREKAPVMQKDAKSDGVEVFPAISFYGGSD
                     FNSGFTVITSFRFPCRLSAGAGIGAEWFKTAGLPVFVEGKYHFLNGSLSPFIYAQGGY
                     TLALAKGQDDQYTDYHGGPLLGAGIGLRKDFTGRNAFVFSLGYRFQQTRTVYDHYYWY
                     SSEYKTERIDRYNRLAFTVGFLFN"
     gene            22355..22651
                     /locus_tag="PKI35_07615"
     CDS             22355..22651
                     /locus_tag="PKI35_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNX44286.1"
                     /translation="MDLSTCGLICNECPSYGKECNGCRAVEGKPFWALEMMPDKTCPL
                     YRCAVNERKYADCGGCAELPCKMFLEMKDPSSTDEEHRQGIIVRVGRLQKSAGA"
BASE COUNT         6148 a         5724 c         4976 g         5873 t
ORIGIN      
        1 ccatgcctcc taccggatgg actattttcg ggaatacatc caactgggtt atatcccaaa
       61 caaataatgc cggcggcgcc ctccccgaag gacgtctcaa aaacagcccg gcatttacag
      121 ggtatatgcg tttaatctcc ccgctcattg accttactgg tcaaacaagt gtaatcatcc
      181 agtttaaaca catgttcgaa ggcgctaact ttattcttgg agttgacacc cgctcaaact
      241 atggagcatg gaataatgta tggacgctta ccccgacagc ttccatttcg ggatcaaccg
      301 tgatcattcc tgtttcaaac gctgacattg gatccaacaa atttcagctt gccctttata
      361 taaacggcag cagcgccaac atgctgaact ggtattttga tgatatccag attctttccc
      421 ctatgcccgt tgatgccatg atggccggct gcgacattcc caacagcttt atgcatgcca
      481 aagaagttac cggaaaagtt gcaaatcttg gagttgccgc tataacctcg atggatgtta
      541 actggcagat caatgacggt gcaattcata ctactcatta cgacgggctt aacctgggca
      601 caggagaaca atacagctat ttctgcaatg actccatcaa cgaagctccc ggcacataca
      661 cgtttaaggt atgggtttcg ggcgttaacg gaagcaccgg ccctgacggc aatcccgaca
      721 acgacctgat cactaaagac attacgatcc atgagtactt cgccctgcgc cgtcccatgt
      781 tcgaagaatt taccagctca acctgcagcc cctgcgccaa ttttaacgca acggccctga
      841 atccctttat tgaagctcac ggtaatgaac tgaccctcgt taaataccag atgaactggc
      901 ccggttcagg tgacatttat taaacggctg aaggcggcgt gcgcagaacc tattatggcg
      961 tcagctatgt tcccgatttg tttatcgacg gaacgcgtgt aaataccgac gccaccggcg
     1021 taacaaacgg cttcaataat tcaatcgcaa aacaatcgtt agtcgccatt aattcaaccc
     1081 acgtggttga tggaaacaac gtgatcatcc atgccatcat atccccttac gatacctatc
     1141 agggtgtaaa agcacacatt gccgttgttg aaagaaagac aaccggaaat gtcggctcca
     1201 atggcgaaac cgaattccac aatgtgatga tgaaaatggt tcccgacgcc aacggcacaa
     1261 gtgtcaacct gcttcccaat cagccggttg agctcaatta tacccaggat atgacggcaa
     1321 ccaacgtaga ggaaatgact gacctgcggg tggctatttt cgtccagaaa tcgaacaaag
     1381 aggtttttga atcgaactat tccatcgaaa ccggttcatt catcagcatc acaccggcca
     1441 atggcgcaac caacatcagc ctgaccgatc ctttgttgat tcagttcagc catcctgtcc
     1501 agatgaaaga cggcagtgaa ctgaccaatt cgaatgccgc ttcggttata atgctgaaag
     1561 aaaataacat aaacggcccc aacgccagct acacggtttc tgtcaatagc gaaaaaacac
     1621 tcatcacgat cacgccaagc cccagccttg ttttcaacaa aacttatttc cttaaagtcg
     1681 acacattgga aaacaaggtc agcggcgttc acaccctggc tactacttcc tccttcacaa
     1741 cccgcagcaa cgttaatgtt cccgaaaacc caacagtggg caaagtcagg gtttatccca
     1801 acccggccaa tgatttcgtc aatgttattt cgggtgaaac cggcggaatt cagggtcttg
     1861 aactgatcag cctttacggg aataccgtac gccagtacac cgattatatc aagggaggtt
     1921 cttcggcaaa actggatgtt tcggaggttc cggccggagt ttatttcctg aaagttctcg
     1981 gtgataacaa acaacaaatg atacgactgg ttatatcgaa gtaaaccggt ccttaataca
     2041 ggcctaaaca taaagaggct gcctccagaa gggcagcctc tttttttatt ccggtttaca
     2101 attatagccc tttttatcgt tattttttca gattcggggc attgccggga tataaaaact
     2161 atccgatgga ataatcatcg ttattattat ttatatttac atatgtttta tttattaatc
     2221 taacaaatca aaaaccatga aatcaaaaac cggaattatt gctgtcaatt tcagcgccat
     2281 cctcttcaca ttactgtttt ataaacaggc aaccggtgtg aaccttctta ttttcgaaat
     2341 actctatttc tgttggttag ccgtatccag acaggttgat ttccgtaaca ggaatatgtt
     2401 ggttttcgga tctttattgc ttctgaccgc tttatttacg gtgattacgc attcggtatt
     2461 ttcctatgta atacattatc tcgcattact ggtttttgtc ggaatactga tctatccgga
     2521 agcaaagtcg ctgataagca ttacaggaat atctttcctg aacataggcg gcgcccagaa
     2581 aaagtttctc gaacatttat cggaatatgg tattaaaggg agccggattg cttccttgtt
     2641 caaacgggga atgatattcc tgtttcctgt cattatcatt ctggctttca tcatcatcta
     2701 ccggaattcc aacccctggt tcgacggcgt tgttacaaac atactaaaac atatcggcaa
     2761 ctggattaat ctcattttca aagatttcga cttcatgatc atcgtcactt ttttgattgg
     2821 gctgctgatc agcaacatac tgctgttaag atggaataac cggaagatca ttgaaaaaga
     2881 cagctccgct gccgacacac tcgaaagaag aaaatcaggg aagcagcatc attttaagcc
     2941 caacggattg aaaaacgaat accgggctgg tgtatttctt ctgtcggcgc ttaacatcct
     3001 catcctggta atgaatgtga tggatattta ctgggtatgg tttcatttta cctggaacgg
     3061 gcaatacctc aagcaatttg tacatgaagg cacctatctg cttattttat ccatcctgat
     3121 ctctatattc ctgatcctgt attacttccg gaaaaacatc aatttttatt caaaaaattc
     3181 cctactgaaa atactgagtt acctctggct agcgcaaaat accatcctgg ccctgtcagt
     3241 cgggattaga aatttctggt atatctatta ttatgcgctg gcatatagac gcatcggcgt
     3301 cattattttt ctggttctta ccctgtttgg actatactcg gtatttatga aagtacttcg
     3361 ccggaaaagc tctttctacc ttttccgggt aaatgcgctg gccatatatc ttgttcttgt
     3421 gatcagctcc gctataaact gggacacgct gatagcccgc tataatttca gccatgcaga
     3481 ccggtcgttc cttcacctgg attatgtagc ctctttatca gacgaagcac tgccttacct
     3541 tgaccacccg ttggctgaac tgaaaaggat tgataccgtc cagaaaaaaa atttcccaaa
     3601 cgagaaggtt atttacatga aacccgatac atatttttcg atcattgaga accgcaaggt
     3661 gtttttcaag aaaaaatggg aatcaaaagg aataatgtca tggaacctgc ccgaatacct
     3721 ggcttataag aacatgttcg ggaaaccgga aaattcactt ggaatgcttt tcttgccata
     3781 gatatatttc ctgacagaaa attcccgtaa tgcgccccga ttccggccgt tggttttctc
     3841 ctttatgctg ccggacagat gcgtttttag tcgatgatga taaccatcat gatcccgggc
     3901 aattaatata tccggaacct gctgagatgc aaattggtat tgtggcaatc atcatccggt
     3961 tgataacaga cagaaaaaac tcataaaaaa ctacctgttc agaatatcat taggatgtaa
     4021 ctaaaaaatg ctgacatttg tcatgtgtat tcattaatac tttccgatca atttactgtc
     4081 gtatgaaaaa attagtaccc ttagttgctt tactgcttgt cttccacgta tcacttattg
     4141 ccggagaaac caggcatacc tacactttcg gtaatcctgt cgtcattaag acaggcagtt
     4201 accaactgat agaattcgac aataccttgc aaaccggatt ggtcggcgaa ccgacgctcc
     4261 cctattgtaa ggtagatatg ttgcttgcgc ccggcgaatc ggcaacacga atcgaatacc
     4321 agtggaacaa cgaaatcaca attcccggca ccttcgtatt atttccgcag caacaggtca
     4381 ggccgatctc gcaagatccg tccggtattt tcctgaaaaa cgaaagcctt tatgctgccg
     4441 acattaaata tccgggaaat gcagggggac accttgtaac atcgttcctg aacggtaggt
     4501 ccttagccct gtcgactttt acaccggtaa ggtacaatcc ggcaacagga caggtttcat
     4561 actttgaaag tgttaccgtc accgtttatt cttcttcttc acccgaaagc cgggaagcaa
     4621 tgaaaaactt ccgtacgggc aacgaaattg aatccagggt aaaaaaactg gtcgataatc
     4681 ctgaagcggc aaccttatac agttctgatg atgcagcagc agccgatgat taccaggttc
     4741 ttgttatcac tacatctgcc ttcagcaatt cctttagcag tctcgtcgat cattacctga
     4801 tggaaggaat ggcttgccag ataatgacga ccgaagaaat caacacttcg gtatccggca
     4861 acgaccttca ggaaaaaatc aggaactata tcacgagtga ataccaacag catggcgtag
     4921 aacatgtgat cctgggcgga gacgatgaac tggttcctca ccggggactt tattgttttg
     4981 ttcagtcaag ttcagcttac gaagatcaga acatacccgc cgacatctat tactccggcc
     5041 tcgacggtaa ctggaatact gacggcgaca gccagtgggg cgagcctggt gaagatgatc
     5101 tggaacctga tatttcggtt ggccgtatgc cattcagcac gctgaatgaa ctcaatgcca
     5161 tgctcaataa aactgtttcg tatcagttta ctcccgtatt gggcgaaatg cggcaggtat
     5221 taatggccgg cgagcacctg tacgacaatc cgaataccaa cggcagtcaa tatatagaat
     5281 tactcattgg cgaccattcc gataacggtt ataccactag cggcatcccc gaaaactatg
     5341 atttcgacaa gatgtacgac gaagtagaat cctggagcgg cagcgacctg atgaaccatc
     5401 tgaaccaggg acgtcccatg ctcaaccaca gcggccatgc caacgagacc tttgtaatga
     5461 agttcaataa cagcgacatc accgacgcta acttctcaca ggttaacggc actactcata
     5521 atttcacggt tgtatatacg cacggctgcc tttgcggcgc ttttgactac aacgattgca
     5581 ttgccgagaa aatggtgacc atcaacaatt ttgcagcggc ctttgtggga aattcaagat
     5641 acggatggtt caacgaagga cagactgaag gcccttcagc ccatctccac cgcgaattca
     5701 tggacgccct tttcaccgat cattataccc gtataggccg tgcacacatg gaatcgaaat
     5761 gtgccaccgc cccctgggta actgccccgg gacaatggga acctggcgcc cttcgctggt
     5821 gcttctatga ttgcaacctg ctgggagatc cggcaatggc tatgtggacc gataatcctt
     5881 ttaatgccac tgtaacttat tcccaggcaa ttccagttgg catcacaact acctcggtaa
     5941 gcgtgcataa caacggccag ccggctcctg atgtaacctg tgccatcgtc aaaaacgggc
     6001 tggttgtcgg gaaaggtata accagttcaa caggtactgc cgatataata tttaatccgg
     6061 ccgtgaccga acccggcccg gcaacactgt atgtatctgg cggcaacagc atgcctgctt
     6121 cctataccct tgaatttatc ccttcaacgg gtgcctacat tgtttaccag tccgttaccg
     6181 taaacgatgc aaccggcaat caggatggta tccccgatta tggcgaaacg gtaagtctgt
     6241 cgttaaccgt gcagaacaac ggagcagagg acgcaaccaa cgtgactgtc acggtaacct
     6301 ccgatgaccc ttacgttaca tttaccgatt ccaccgaact gtacccgagt gttcctgtca
     6361 acagttgggt taccattgac aatggtttta cattcgtggt aagcgacagc attcccgata
     6421 tgcacaacat cattttcaac gttaccgcta cttcgggaaa ttcatggtat tcaaccttct
     6481 cgcttacagc tcatgcgcct ctgctcgtaa cgggcaatct cagcatcagc aatgagagcc
     6541 tggcacaaaa cggacgcctt gatccgggag aaacagccac cgtcaacatc cctgtcagca
     6601 accagggaca cagcgattgc agaaacatga cagcttcgct tgcatccgac aattccagcg
     6661 taacaatcgc taatccttcg atcaccctgg gcaatctgca gaaaggagga accgttacgg
     6721 cttcattcca gacagaggtc ggtgcaacca tcccgatgta ttccctggtt gaacttgatt
     6781 ttaccgctgg aagcggtaat ctttccgttg aagaacagta ttaccagcta ataggtgtga
     6841 atgtggagga ctttgaaaca ggcgatttta cgaaatacaa ctgggatcag ggcgatcatc
     6901 cctggatgat caccagtgaa tcgacatttg aaggagacta tagcgcacgt tccggcgtta
     6961 ttgccgacgg cgaaacatcc cagttcagta tcaccatgga agtacttgct tccgacagta
     7021 tttcttttta ctcaaaggtt tcatgtgaag atacttttga taaactatcc ttctctgtta
     7081 atcagcagga aaaagatgcc tggtcgggag aattgccctg ggagcatcac agttacatgg
     7141 tcgaggccgg tatgaatact tttaaatgga gctacaccaa ggatttccat gaaattgcag
     7201 gaagcgattg cgcctgggtc gattacataa tcttcccggc tttcaggaat aatgccggcg
     7261 ttgatgaaaa ggaaataccc ggaattgatt tatgccttta tcccaatccg gcttccggag
     7321 aacttcacat aaaatgcagc catctgaaac ctggagacaa caccctttcc ataattgcgg
     7381 ccgatggcag gatatgttac aaacaatccc tgaccggctc ggcggaaacc gtggtgaaca
     7441 cctcttcact taaatcaggt atttacgagt gtatcatttc gaacggcagt gtccatcagg
     7501 tatcacgtgc ttttatcatc cgataaagta attacagcct gaaggaaaca aaacatgatc
     7561 atataaaacc ggctgacgtt tcgccagccg gtttttatta tctcaccgtc agaaatttta
     7621 ataccctgat tccctttccc ttaaattgac aaataaccgc gtattaagat tttttttatt
     7681 tgaattgatt ctaaattaat cggtgatata aaaagcattc agccaagaaa ttacattttt
     7741 aagaaaaaaa tttacttttg ttaccttttg tttccacaca gattaaaaaa atatttcttt
     7801 acctacaaac agtcaaaacc aattcacaat ggccttaaaa tctacacaca cgtattgggc
     7861 tttactcgtg actttctgcc tgacgatatt cactctcagc agttcggccg aaaaagttac
     7921 ctatcaggga agctggggaa gtccaggcat ctcattgcaa aaaaacgatg cgcagggagt
     7981 aaccatcgga ttttccatca aggaattcga tatttcttcc cgttcgttca acggagaaac
     8041 gatgcaggag atcgggctta ccgggaattt tctgccaaat gcagaaggcg ctcccaacct
     8101 gcccgtcatc agcaggtatg ttgctatacc gcaaggatcg caggctgcta ttgaaattaa
     8161 aaggcagcgc accgaaacgg tcagcaacat cgacatggca ccgtcgccac gtataccgct
     8221 tgatacggaa cgcggaccac tccagtacaa taaagacctc agcatttatt caaagaatgc
     8281 cctgtatccc caacaaccgg tgatagtttc acaacccaag aaaattcgcg gtgttgatgt
     8341 ggttttagtg agtatcactc cttaccagta taaccccgta acgaaagaac tggttgtaat
     8401 tcgcgatatt gaagttaatg tgaattttac cggcggcaac ggtcattttg gtgaagaccg
     8461 tctgcgcagc cgtttctggg atccgattct gaaagacaac atcctgaaca gcgaatcaat
     8521 ccaacccatt gattacagcg cccgtatggc ctaccagacc tcacaccgtg aaaccggctg
     8581 tgaatatctt atcattgttc ccaacgatcc catgttcagt caatgggccg atacgatcaa
     8641 gaaattccgt accgaacagg gtatccttac taaaattgtt accttacagg aaatcggcag
     8701 caatactaca acggctattg aaaattacat caacaacgct tacaataact gggatattcc
     8761 tcctgcagca tttctgatga tggccgacca cggcaccaat gcacaaactc agattcctgc
     8821 ccctatttgg gataactatt gcgtttcgga caatctctat gccgacgttg ataatgacga
     8881 cctccccgaa attgcccatg cccgtatgac agcccaaacg gctgcccatc tgcagacaat
     8941 ggtgaccaaa ttcatcgaat atgagacaaa tcctcccaca agcgccgatt tttacaataa
     9001 gcctatcacg gctctcggtt ggcagacaga acgctggttc cagatttgtt ccgaagttgt
     9061 cggcggtttc tggaagaact cattaaataa acagcctgtt cgtatcaatg ccgtatatca
     9121 gggcaacccg aattccgatc cctggtcaac agctaccaat accgctaccg ttgtcaatta
     9181 cttcgggcct aacggaaccg gttatattcc cgccactccc caggaactgg gtggttttag
     9241 cggaggaacc ccttcccagg taaacgacgc cctcaatgcc ggatccttca tgcttcagca
     9301 ccgggatcat ggttatgaac aaggttgggg cgaacccggt tatacgtcaa gcaatattcc
     9361 aggcttaacc aatgttggca agctttccta tatcatgtcg gtaaactgtt tgaccggtaa
     9421 atacaattac agcagcgaag ttttcgcaga aaaattccac cgctatacct acaacaacca
     9481 aaatgccggc gcagtgggaa tcctggcggc ttccgaagtt tcgtactctt ttgtaaacga
     9541 cgtctttgtt tgggggatgt acgataacat gtggaccaat ttccttcccg attatggtgc
     9601 tttgttcgaa gaaagggacc tgattccagc attcggaatg tgcgcaggca aaatattcct
     9661 gcaacaatcg tcctggcctt acaatacttc caacaaggaa gttacctata acctgttcca
     9721 ttgccatggc gatgctttcc ttgacgttta ttcagaagtg cctcagcaac tgaccgtaag
     9781 ccataatgaa gtaatgctgg ccggtttaag cacctttgat gttaccgcca atgaaggcgc
     9841 tttcatcgcc ctgtccgcca acggagaaat tatcggaaca ggagttggta ccggcagtcc
     9901 tgtcgctatc ccggttaata cccctgctat cggaactgtt gtaaagattg tggttacaaa
     9961 acagaattat ttcaggcata cctcaagtgt aatcgtaatc tctcctgaaa atccctatgt
    10021 tgtctcaaca gccgttgcca ttaacgaccc ggccggaaac aacaaccaga aacttgattt
    10081 tggcgaaacc gctttcctgt cagtcaccga agaaaacctc ggcaacacag attcgcagaa
    10141 taccctggta accctcaacc ttaccgatcc caatatcacc atagttgata ataccgagaa
    10201 ttacggggtt gtaccggcac atcaaaacgt tacagtaccc agtggattcc aggttcaggt
    10261 tgcaaataat gttcccaacg gatacaccgc aaccgttcag gctacagcaa ccaatggcac
    10321 tgatacatgg gtcagcaatt tagcgcttac ctgttatgct ccggctttgt cggttgccgg
    10381 caccattgat attgacgatt cccaatccgg aaacaacaat ggaaggctcg acgccggtga
    10441 aacagttgat cttattatca acaactacaa caacggcggt gcaactgcct taaatgcaat
    10501 agcatctctc gcaagttcaa gcggttggat cacgatcaac tctgccacct ataacgtagg
    10561 agacatagct ccccttgcat cgcctaccgc tgttttcaat gtaacagctc atccaaactg
    10621 ccctgccggc ctgcatattc ccattgacta tacgttgacg gcaggaagtt ataccctcga
    10681 gcatgtttat gatctccagg caggtatcat tgttgaagac tgggaaaccg gcgacttcac
    10741 caaatttagc tggattgccg gcgggaacca gccttggacc atcacaaatg ttgctccttt
    10801 tgaaggaacc tattctgcaa aatccggtac gattaacaac agccagactt cacaactgat
    10861 tctgaacttc aacgttccgg ctgatgatac cgtcagtttc tattacaaga cctcatcgga
    10921 agcatcaaaa gacaaactcc gcttctacat tgatatcatt gaaaaaggac agttttcagg
    10981 tattacagga tggcaacagg ccaaattccc catcacggcc ggttcccata ccctgaaatg
    11041 gatctataac aaggatgcat cgggtagtgc cggaagtgac tgtgcatggg tcgatttcat
    11101 tgttctgcct tcagctccca caacagcctg tatggccggg cctgatggtt attcctgtca
    11161 gggtgctcct ttccaatgcc agggagaagc aataaattac aacactgtgg cttgggaaac
    11221 atcaggaaca ggtaccttta gcagtcaaac cattctgaat ccggtttata cgcccagcgc
    11281 cgaagacata gctgccggat cggtaaccct tacaatggct gctaccggca tctcgggtac
    11341 tgtggtcgac aacatgctcc tttcgatgca ggatccggct accgccaatg caggcgccaa
    11401 tggaagtacc tgccagggtg catcctactt tcactcaggc gccagcgcta ccaattacaa
    11461 ccttcttgaa tggacaacca gcggcaccgg tacgtttgac aacattcacc agctcaaccc
    11521 aatttacacc cccagcgcag aagatgctgc agccggaagc gtaacattga cgctgaatgc
    11581 tatcaaccag ggatgcggaa cggttacatc ttccatcacc ctggtcgtta atccgttacc
    11641 agctcctgca ataaccggtg aaactaccgt atgccaggaa acacaaaatg tggtctattc
    11701 aactccttat gtaaccaata attcatacac ctggacagta acaggcggtg aaatgacaac
    11761 aggagcaaat ccgaacgaga ttgccgttac ctggaatgaa gccggaaccg gaaccgtttc
    11821 ggtggcagaa accacaccgt tcaactgcag caccacagcc gtagccgacg tagttgttaa
    11881 tgctgttccc cagcctgaaa tcagcggaaa cgttgctccc tgcgctggtg aaacggttac
    11941 tttcaccact ccccaggtgg aagggatcag ttatacctgg gatgccggcg cttacaacat
    12001 cacatccggc caatcgacga acgaaatcca ggtttatttc gatatccccg gcactcagaa
    12061 tgtcatggta accgaaacgg ataacgtttc gttatgcagc aaatccgccg ttgccgctgt
    12121 cagtatcaat atcgttcccg gaactcctga agttccccag ggccccgacc gtgctgacct
    12181 ttactatgtg acctctaccg acgtgaccgt aaccgctgta ccttacactg ctgtttatac
    12241 ctggcagata tcgcctgctg aagccggcac cgttgccaac accgataata acgctaccat
    12301 cacctggagc cccagttatc gcggcaatgc cgaagtatcc gttaaatcgg gcaacggttg
    12361 cggtgaaggt gcctggtcac cggttaaatc tatagaagta ctcaattcga ccggtatcgt
    12421 tgaacccgga aagaacctcg gcgttatcgt tacaccgaat cccaacgacg ggaagttcaa
    12481 ccttactatt gtaacccccg gtacttccat gatcggctac cgtattgtca atgcaagcgg
    12541 agctactctc ctggaagctc acggactgca atccaacggc aaatacaccc agcagatgga
    12601 aacccatctt accccgggag tttacagcat aatcgttaat actgttaacg gacaagctgt
    12661 cgaacgcttc gtggtgaaat aaacgatctg acgtacacaa ctgaaaaagc cggtccaaat
    12721 gaccggcttt ttttttgaca ttttccaagc cctggtttga ataaaatcag gaaaaaattc
    12781 aattatttga cgttcagggt atcacttatg agtggaggag ctattaatta atactaagct
    12841 tgtttactcc tttactcatg aagcaccctc tggtactcaa cctgcttgta gcgctctttc
    12901 tatccatagg gataaaaagt tatggcaccc ggacgtgggt gtctgttaca tccggcgctt
    12961 cccgtcctgc ttccctccag actaaatctt cagtgaatgg atgtgttatg attgacgcat
    13021 ccatttccgg cttttatacc aatcctgttt cgatcaacgg cgccgaatat ctggttgttt
    13081 ccttgccagg ttcatatcct tcgcttgaag ccgggaaccc tggcctcccc tatttaaatg
    13141 caaccgtcaa tattccggca aacggcaggt tctcaacgcg gattattgaa tcggaatacg
    13201 ttgaatatca caacatgcgt atagcgccat ccaggggaaa tctcccgttg accgctgaag
    13261 ccgctgctac tccttataca ttcggcgaat cctatgatca tgacgatttt taccccggga
    13321 accttgccgg cggacaagaa acatttataa tccgcaacca gcgcggacaa tcattcagca
    13381 tcaactcttt tcaatacaat ccggtgaccc gtacactcag ggtttatacc aaactctctg
    13441 ttgaaattta cctgtcggaa acgcagggcg aaaacgaact ttcgggcgac gaccttaaca
    13501 tttctaatgt ccgggaaata acagcttcgg tgcagccggc attccttaat aaccagactc
    13561 gttcagatgg tttaccggac gaaaacgggc agatgctcat tatttgtccc gggaattttt
    13621 cagcatcatt aaaacctttc accgactgga aaaatgccac aggcattgcc actcatgttg
    13681 ttgatgcagg atcgttcaag acccctgaag aacttcgcga ttttgtcagg gactattatt
    13741 atgctgccgg gaatcttgca tacctcttgc tggtgggaga tgctgaacag attcctccct
    13801 atatcaagga tgacatcatc tctgacaact attatggtta ccttgccggc gacgatcatt
    13861 atcccgaagt gctggtggga agattctcgg ctgagaatat caatgaactc aacacacaga
    13921 tcgaccgtac ccttgaatat gagaaaggtc tgggaaacaa ccggaattat tattccactg
    13981 ctaccggtat tgcatcggaa atgaaaatgg gcgacgacgg tgaaagtgat tttcagcata
    14041 cccggaatct cctgagtacc cttaaagatt tcacatacac tgattttgca gaattatacg
    14101 acggttcaca gggagggctg gacgccgatg gttaccccac ctcacaaatg gcaattgacc
    14161 aaataaatca gggaacaggc ctgatcttat acacgggcca tggcggcata aatacctgga
    14221 ttacggccca ggtttcatca tccgttgtgg atatgctcgg aaataatgga agttatcctt
    14281 tcatctggtc agttgcctgt gaaacgggaa atttcaaagg tcatacctgc cttgccgaaa
    14341 gttggctccg cgctaccgat aacaacggta aaccggccgg tgcggttgcg gcattgatgg
    14401 catcgggagt gcaaacaacc atgcccccta tggaagccca ggataaaatg gttgattacc
    14461 tggcgcattg ttcagaacaa ggaagcccaa ggactttcgg cggactgtcg gcaagcggaa
    14521 tgatcagcat gaacagtgca tatggtaaat atggctatgc gatctccgac acctggattc
    14581 tcttcggaga tccttcgctg gcggttcgta ccaacgcccc tgaaccgata ctagccagtc
    14641 ataagtctta tattgccgaa ggcggagtgg tttttaatgt ttcggccaat gtatccggcg
    14701 gacttgccac cctttcgcag gatggtgaac tgttggggag tggaaccatc tcacagggca
    14761 aagcgcaaat cattcttcaa tcgccggcat cgggcagttc tattctgctg acgattactt
    14821 ctttcaaccg gactccctat acttccgaca ttcttgtacg gaataaacca tctgatgcaa
    14881 ctgctgttac gccggtgaac cacagccggt taattcccgt tgaccccgta tttgaatgga
    14941 atgccggcga cggcgcccaa cctgatggtt tccggctttt cctgggtaca aacaatcccc
    15001 cgtcaaatat catcaatggc tttgacagga aagactcttc ttttaatccg aaccttcacc
    15061 tggattacaa taccacctac tactggagag ttgaatccta caatgccaac gggtcgtcgt
    15121 acggcgttgt aaatgagttt aaaacagtct tccagcccga tgaagatttt gaaacaggcc
    15181 agtttaaaag cacctcggca tgggtgaatg aaggtggaag ctggactatt gataaccggg
    15241 acgctttcga cggagatttc tgcggccgtt cggggataat aaccgataac ggatattcgt
    15301 cactcaagta ctcctgcaac gtcaattcat gtgattttgt gagtttctgg aggaaaacat
    15361 ccaccgaaga gaacaaagac aaactttgct tttatattga cggccaattg acaggcgaat
    15421 ggagcggcga aagcgaatgg aagcatgaaa tattccacgt tacagcagga tttcatgaac
    15481 tggaatggag atattccaaa gatgccggca atggttccgg cgaagatgcc gtttggcttg
    15541 atgatattca tctgccggta cacggctcat ttgaagccgg cactacgccc ctggggactg
    15601 tctgcgccgg atctccgttt gttcccgaag cctccgcatc aaattacagc agtattacat
    15661 ggcaaacaac aggcgacgga acctttgacg attgcaacct ccttattccc caatacagcc
    15721 ccggtccgct ggacctttcc acaggatcgt tcctgctaac catgcaagcc gacggctata
    15781 acggctgttc aagctatacg ggaatggtgg tcggaaatat taatcccaca cctgaaatca
    15841 tgcttccgga ggataccctg gccagcgccg gcggatcaat tgtccttgac ggctcctgcg
    15901 aaagcggcat tgcttatgaa tggattcaaa ccgggacaac tctaccctat atcactgtaa
    15961 atgaatcgga tgccgccgac ggaaaaatca gtctgacact taaagtaacc aatgaatacg
    16021 gatgttcgag cgaacagaca accacggttc atttcgtcaa acccggtgat aagccttcgt
    16081 tttctgttta tccgaatccc tgtaccgatt actttaccct tcagcccgag aacgggctca
    16141 tggcgctgaa ccacctgtat gtggtagatg cccggggcac gatacgctgg caacagaatt
    16201 cggacctgac tataaacggg agttcaactt tcaatctgcc tcagctcgcc tcaggactgt
    16261 atatggttgt tgccgaaagc gaagcaggcg ccgtaaccag gccgatactc atcaaccctt
    16321 aatccaatct gctcaattac ttccttgata gccggaaaaa atccggcttt gttggtttcc
    16381 accaacttcc tcagttaata tttcaaaata cggagatcta atcgccttat atctttcagg
    16441 tctatgaaaa atagctactt ttggcgccta tctcaactgc cattactatg aggatacgtt
    16501 tctacgctgc aatctgcatt cttctctccg ccatttctgt catggcacaa caggctgatc
    16561 cgaaccatgg cctgaaacac tggatgaccc ccgaggaggc ctcgcaccgg gacctgatag
    16621 gaaggggttt cgtggaaacc gatcccccgg ccggcatcgt ccgcaatgtt gaagaatttg
    16681 gcccggctga aggcgtattg gtaagatacc cgttcgggat tccgattaat ctcatcaagg
    16741 aaatggcaga agatgatacg gtaacaacca tcgtcggaaa tgcatcaaat caggcatctg
    16801 tgacttcaca ataccagtcg aacggcgtca acctggcgca ttgcaagttt ctgattgccc
    16861 cgacagatac atactggacg cgcgattacg gcccctggta tgtgacttac ggcgacaaac
    16921 aggtgggaat tgttgatttc ccatataacc gtccccggcc caatgacgat gatattccca
    16981 aaaaacttgc aacttcgctg ggcatagaat ggttcggaat gaatgtgatt caaaccgggg
    17041 gcaattatat gaccgacggc atggggcaat cggcctcaac aacccttgta tgggaggaga
    17101 atccgggaca aagtcatgaa cagatcgctc agaaaatgca tgattatctg ggcatcgaaa
    17161 attacatggt cgttcccgac ccaaacggga cctatattga ccatattgat tgctggggca
    17221 aatttttggc gcccgataag attctcatcc ggaaagtccc cccaacgcat ccacaataca
    17281 cccttatcga aaatactgct gctttttttg catccagtat ttcatcctat ggaacgccat
    17341 accgggtatt cagggtcaat actccgaatg acgagccata cacaaactcc ttcattctga
    17401 ataaaaaagt attcgtgccg atcatgggga atagcaacga caacgccgca ctgctggctt
    17461 atcaaaccgc tatgcccggc tacgaaatac tcggatacct tgccaaccct tcagcgccct
    17521 gggaatcgac ggatgctttg cattgccgca cccatgaaat ggctgaccgc ggaatgctct
    17581 atatcaaaca catgcctttg ctgggacaag ttactgcgca ggctgatttt gagataaacg
    17641 ccgatattgt tccattcagc aaacaacccg tttattccga ttctgcctgg gtaatctata
    17701 aagtaaattc cggggcttgg gatacccttt caatggttaa ttccgctggc gatcattgga
    17761 cggcatcaat tcccggtcaa atggaaggcg ataccgtttc ctatttcatc acggctgccg
    17821 atgcttcagg ccgcaggcaa aaccatcctt ttatcggcgc tcccgacccg catgtgttca
    17881 tcattcaggt attttctccc ccggatgtta ctgtagcccc cgatacgttg cagtttgaaa
    17941 catgggaaga tatgtatcac ggaaagaagg ccctggttcg taactattct tcccaaccgg
    18001 tcacaataaa ccggattaac acacagggaa cagacccttt cggctggtat atcgatccat
    18061 ggaacatcga accgcctttt atcctcaaca gcggcgattc attgatcctg aacgttaaaa
    18121 ttgacctcca ggccggcaaa accgatattc gctgcgatac cttgcaggtt tccaccgatg
    18181 cttccgttca caatgttttc ctctgccttg acctggattt gcttacagga ataaatcccg
    18241 gcgacggaac atcctctgaa ccggaagttt tcccgaatcc cgtacaaaca acagctacgg
    18301 tcggtttttc aatcgaaaaa gccgggcgcg tcaaaattga tgttatatcg ggcacaggac
    18361 aggtggccgc cacgattgct gataacaatt tcagcaaagg gaaacacagc ctgacgtgga
    18421 acgcacaaaa gttgccttcg gggatctacc tggtgcggtt tgatgacggg cacactgtcg
    18481 gctacaccaa agtgttggta aacaggtagc cggaatcatc tccttccttt tttcgcaggt
    18541 tcttcggtta taatcctgca tcttagccgg ttcatgataa tgcaggttgc tgtttatctt
    18601 tcaattaacg acagcctgaa gttgatggag tattatcatt ttagtatgat aaccgtttaa
    18661 aaggaacaat caggagaaaa atgttttaca ttagcatcgc ttaacctcta atttcccgaa
    18721 ccttgttaaa aagaaaagat gttcaggcca taaaaaaagg cgataaaaag cttgtaaatg
    18781 gctgggtaat gtacgactgg gccaattcgg tttaccagct taccatagca tcggctattt
    18841 tcccggttta ttataacagc gttacccgta ccgacggcga tttcgtggtt tcgtttttcg
    18901 gagcgccggt catcaatact gtcctttatt cctgggccat agcggctgca tacctcattg
    18961 ttgccatggg atcgccgttg ctttcatcaa tggccgacta taccggacga cgtaaaggat
    19021 ttatgagagc ttttacgatg atcggagccg ttgcatgcgg agccttgttc tttttcaata
    19081 aaaataccat tgaactggga attattgcat ttgccttagg cacggttggc tacggcggaa
    19141 gcatcgtttt ctacaactcg ttcctgcctg taattgcaga gccaaaggac caggaccgca
    19201 taagcgccag ggggtattcc atgggctatc tgggcggtgt cgttttgctg cttttcaacc
    19261 tgttgatgat catgaagcca ggctggtttg gcatttccgc cgacagcgac ctgccggctc
    19321 gcatttcctt tatcactgtt ttcctttggt ggattggctt ttcgcagatc tcattcaacc
    19381 gtctgcccaa gtatactttt cggaaaagga tgaaccgggc caaagtgctg acaaacggat
    19441 accgggagtt gcgggtcgtt ttcaaacagg tgagaaaatc atttcaactc agtgtatatc
    19501 ttatcggctt ctttttcatg atgatgggac tgctgactac catgtttatg gcggcaactt
    19561 tcggcgaaaa ggaagtaggc gttccagggg atattctcat cctgactata ctcattatcc
    19621 agcttgtggg aatgttcggg gcatggctgt tttcgcgtat ctccggaaaa attggaaacc
    19681 tgaaagctct ggtgatctca gttgtaacct ggattttaat ctgcgtttat gcttatacca
    19741 tcaaggatac tgcaggcttt atgatcgctg ccttctttat cggcatcgtg atgggcggtt
    19801 cgcaatcgct ggcccgttcc acctattcga aaatgctccc cgaaaccacc gaccatactt
    19861 cattcttcag cttttacgat gtaatggaaa aactggccac cgttgccggg actttcagct
    19921 ttggaattat tgaagccctg accggcagta tgcggttctc ggtgctggcc attaccagtt
    19981 tctttattat atcccttatt ttcatgctga tgctcatgcg cagggcaaaa aaaagtgaga
    20041 tggttatata acctatccgg ccgaattgtt gctgcatcca tatcgtcata aaaaaaaccg
    20101 gctcctttcg aagccggtgt catcccggat aaccaactaa ccaccgatgg gaatcctgct
    20161 gatcagggtg atgttattca gatcggaata atcgtactgg taaataccat ccgttgccag
    20221 caggatcaat aggttgccca cggggataac atcatgggcc gttacatcgg taaatgtctt
    20281 taacaggttc tcatcaatgc gcaggggatc actcgcgtca taaatcttca aaccggctgc
    20341 accgtcgcat acaaacagtt tccggttatc gatccccagc ccccagggtt cagtcatgga
    20401 gtagcccttt accagttgcg gactcaaaat ttcccggata tctatcactt ccataaggtt
    20461 ctgccagttg ccgcagcgat tcccggcgcg ttcggtcaca taggcatagc catcctgcac
    20521 caccacaggg tcgcagcttt gaaaatggtt gtactccgaa atgaatactg gtaaagccgg
    20581 cgacgagatg tcgtaaatca gcatgccggt ttgtgtaccg ataaaaaggt gtgttccgtc
    20641 aataaatacg gtttcggctg atcgctgaag atcgattttt tttccgatct caggttttgt
    20701 gggcgcggtt atatcaacaa cctgcatatc cgtttggttc agcccgtaaa actggttgcc
    20761 gtaaataatg aatcgcgcca gcgagccgcc cacaccgatg gtttgcgtgg cataaccacc
    20821 ccctccgccc gaagaattgc ctccctccat ggtataaata tttaatccct tatcgtacca
    20881 ataaacgggg ttgatgttgc cttccacttc ttcggtgaca gttcgtactt tccacccggt
    20941 aatcaccccc ttgttctggt cgatggaagc aatgggatag ttcacattta tatcttctat
    21001 ttccggaatc gtatacggaa aaatatcttt aagccgggcc acttctttcg gatttcccag
    21061 gtcgctgata tccatcacaa ccaggtcgac atagctgtcg gcaaaaagat aatcgccggt
    21121 aacggcaata tccacgttgc caggaatttg caaagtggca atattttgcg gatttgccgg
    21181 attcgtattg tcgaaaatat gaacgccctt gtatttttca ttaacaaaaa ggtagttgtc
    21241 cctgatgcat attttaccct gcacttcaac cgcggtagct cccgacgatt ttacaggctg
    21301 gcgcatctcg tcgtacgaca aatattcggg gacgttcgcc gtaaaagtcc ggcgttcgat
    21361 aaaggtatcc ctgcagccgg cgaatagccc ggaaaggatc agcatcagaa aagcaatttg
    21421 ttgtgttttc atgatatctt cagatttatt attccacttc ttcaaactga ctgcggcaac
    21481 cggcattcag ggtttagttg aaaaggaacc cgacagtaaa agcaagcctg ttgtatctgt
    21541 cgatcctttc ggtcttatat tcggaagaat accagtagta atgatcgtaa acggttcgcg
    21601 tctgctggaa acggtatccg aggctgaaca caaacgcgtt tcggccggta aaatccttcc
    21661 gtaacccaat accggccccc aataacggcc ccccgtgata atcggtgtat tggtcatcct
    21721 gtcctttggc cagggccagg gtatatccgc cctgggcgta tatgaaggga gaaaggctcc
    21781 cgttcaggaa atgatatttt ccttcgacga acaccggcag ccctgctgtt ttaaaccact
    21841 ccgcccctat tcccgctcct gccgataacc ggcagggaaa acggaacgat gtaattaccg
    21901 tgaatccgct gttgaaatcg ctgcctccgt aaaaagaaat ggccgggaac acttccacgc
    21961 cgtcggattt tgcatccttt tgcataaccg gcgctttttc ccggaaaatg atatggtcta
    22021 tttccttttc aggaattacc aggatattag aacccaccat ttcaatcttc acataatcgc
    22081 caaccacatt ctctatgatc ctgccgcgta ggatactccc gttaccgagg taaacggcat
    22141 ccttgctaac ctgttgtgcc ggtgatgcca ggcccgatgc aatgattgtt gcaatcagaa
    22201 ttataattcg taatattttc attgtgaccc gaatgatctt ataactataa agggcaggaa
    22261 aggatcagga tggttgtatt atatcggaca aatattcaga cagatgaaaa attgaataaa
    22321 tttgttacta tcataaaaca aaaaccggga atccatggac ctttcaacat gcggattgat
    22381 ctgcaacgaa tgcccttcgt acgggaaaga atgcaacggc tgccgtgccg ttgaaggtaa
    22441 acccttctgg gcgctcgaaa tgatgcccga taaaacctgc ccgctttacc gttgtgcggt
    22501 aaacgaaagg aaatatgccg attgcggcgg ctgcgccgaa ctgccctgca agatgtttct
    22561 cgaaatgaaa gatccttcct caaccgacga ggagcaccgg caggggatca ttgtgcgtgt
    22621 gggacggctg caaaaaagtg cgggtgcatg atataactga ttataaattc ttcattttgc
    22681 gttgaatgtt tttaattttc aaaatgcaga acattaaaca c
//
LOCUS       DAOPYV010000048        22471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_2340_length_22471_cov_12.673849, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000048 DAOPYV010000000
VERSION     DAOPYV010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22471
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2340_length_22471_cov_12.673849"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..195)
                     /locus_tag="PKI35_07620"
     tRNA            complement(123..195)
                     /locus_tag="PKI35_07620"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Thr,seq:cgt)
     gene            complement(233..309)
                     /locus_tag="PKI35_07625"
     tRNA            complement(233..309)
                     /locus_tag="PKI35_07625"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(273..275),aa:Pro,seq:cgg)
     gene            complement(417..5219)
                     /locus_tag="PKI35_07630"
     CDS             complement(417..5219)
                     /locus_tag="PKI35_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNX44287.1"
                     /translation="MGILDMRTVLVCYMLTSIVSTGVATALWQQNRNRFPGTFFWVML
                     FALQTVSFILIALRGLIPGWLSIFVSNILMILGAYLGLAGLQSFAEYRGRQIHNYIIL
                     GIYTLITAWYTFIHHDLAIRTINLAGALVVFCSQGAWIVFRLKSAEKRKLMLGIGLVF
                     AFFSVINVVRILEVVFFKPGATNFLYPGILEVTIVISYQLLSLILIYYLALMLNQRLL
                     LNISMEERKYSKIFNSSPYCILLTRLSDGKILEVNDGFLKTSGYLYSEVIGKTTVDIN
                     LWNRQEDRSWLVGELLKNGKAQQKGFHFLIKNRNLMIGDYSGEVITLNNEECILSSIN
                     DVTEFRRVESKRLQLLDIIEHSLNEIFVIDSDSLKFLYANQRALLNTGYTLEEIQQLT
                     PFSLNARYSETDLKELFEQLAEGKEIAVFVNTVHRRKNGTEYPVEVTIQFHKQENQDI
                     FFAIVNDITKRRQAEESLQVTLQKLKFYIENTPLAVVEFNANNQVVFWSGNAAKMFGW
                     QADEIMGKSYHELHWIYEEDVTNVTDFSAEMIAGQRTGYSHTHRNYRKDGSVITCVWH
                     NSVLYDPEGALISVYSLVEDITEKKKAEEILRESDERFRTVAILSKSLVYDLEVETGK
                     VKWDGAIEQITGYTPAEYENFGIEQWSGMVHPEDRTKTLLEFNKSLESHLPFKAQYRY
                     QKKNGEYCWIEEESNLIQSPVNLSCRYLGIMKDITVRKQAEEALIASEALLSTVFNSS
                     PIPIAITNLKDNTFVSVNNAWLEITGYQSQEVIGHSPVELGLYADKSRRQAMLDLLHD
                     REIARGFEIEMRTKGGDFISLLMSAVYIKVKGEDCILTMALDNTERKRTEEQLLIKDF
                     AIESSSSAIGLSDLKGRLIYANDAYYNLWGIKNREEVAGKEIYDDDQYREQIEEILAE
                     VKAKGVYSGEKKAFKKDMTPIDIQFSVNFVISPDGSPVCIMATFNDITERKSAEEAIR
                     ESEDRFRMVFENVFDGISIYEEGTERSEKKLVDCNERYAEMSGRSRAELLEFGNTLPL
                     QVPVEKATGISGKGPQGPSEAYKGLFSWLRPDGKENIIEYIGLPVNWHGKSVSIGIDR
                     DITESMKAEKLIRTLSKAIQQSPSSIVITNADGKIEFVNDQFSSIMQYSMEDVKGRNP
                     RIFNPGHIPEEEFSAMWRTLRSGKIWRSEFMNRKKDHTIIWENLIISPILDANGTIVN
                     YILIMDDITEKKRMLDDLIKAKDKAEESDRLKTAFLQNISHEIRTPMNAIVGFADLLN
                     DTEIIAEKRRQYTDLMLQGSNQLLAIITDIVNIATVEAGQARVNEKDVNVNDTIKVLY
                     EEFRVLAQRKNILLHCKTALPDQEAVIVTDQAKLTAILGNFLSNAVKFTEKGYIKFGY
                     NLKAGMLEFFVKDTGIGIPSDMHDKIFERFRQVETNVSRRYGGTGLGLSISKAYADLL
                     GGEIRVDSEPGIGSVFCFTLPYISSLREAIPKEQPVSALKIEADGLKTILIAEDEDTN
                     FALLEEMLSASPVKIIRAVNGAIAVEQCRLNPQIDMVLMDIKMPEMDGCEATMLIKEF
                     RPQLPVLAQTAYADPNDIKRIMACGFSDFISKPFKLEQIISGINRYMK"
     gene            complement(5595..5667)
                     /locus_tag="PKI35_07635"
     tRNA            complement(5595..5667)
                     /locus_tag="PKI35_07635"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5632..5634),aa:Lys,seq:ctt)
     gene            5782..7122
                     /locus_tag="PKI35_07640"
     CDS             5782..7122
                     /locus_tag="PKI35_07640"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HNX44288.1"
                     /translation="MKRTAFLAAILLISMTAAESQTVNKNYMANEVKKAFSHAWEGYK
                     QYAWGYDALKPLSRQGHNWYQQSLLMTPVDAYDVMKIMGLEKEAAEAKQLIFGKLNFD
                     ADMEVQNFEVSIRILGGLLSAYELDGDTGFLNLAKDLADRLIVVFNSPTGMPYRYVNL
                     KTGKVSGPVSNPAEIGTYIIEYGILSHHTGDPKYYETAKKAMVKLHSLRSAIGLPGAG
                     INVETGEWTNTESSISGGIDSYFEYMLKGAILFRDKDLREMWETSAEAVNRYMADTVS
                     GNLWYGRVDMNTGKRTRTIYGALDAFFAGSLALDGDLQRAAMLQESNFKMWMFTGIEP
                     EVLDYSKMEIRYPGYVLRPENVESAYYLYHFTGDEKYLRMGKEMFDNLVKYCRSETGF
                     TALKNVMTKERDDDMESFFFAETLKYFFLLFDDGRSLDFDKVIFNTEAHPYKRF"
     gene            complement(7171..7911)
                     /locus_tag="PKI35_07645"
     CDS             complement(7171..7911)
                     /locus_tag="PKI35_07645"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HNX44289.1"
                     /translation="MPVYNTILDKAVKGRKQLAVLVDPDKLSLKEVAALASRAGDTGA
                     DYFFVGGSLLVNNQLDACIKTIKENANVPVVLFPGNTMQLSWKADAILFLSLISGRNA
                     EMLIGRHVISAPYLKVSPLEVIPCGYMLIESGRQTAVQYMSNTAPIPADKNDIAICTA
                     LAGEMLGLKLIYLEAGSGAQRPVTAGMVKQVKESIGIPLIVGGGIRTPETASEICKAG
                     ADLIVVGTALEKDAGLLGELSSAVHGRQ"
     gene            complement(8113..11088)
                     /locus_tag="PKI35_07650"
     CDS             complement(8113..11088)
                     /locus_tag="PKI35_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNX44290.1"
                     /translation="MKINLLFLALFLAVLSSAGQTAADYYLPFKVGNRTMLQTSSASG
                     SWCFRNTEYKYRWMEEVNGVDYFVEQGWEGNYTTGNCTGDLYFRYLWIRPDQEGNIVA
                     GAYSMAGDDNGASPSLVNAVIPSTPVIIFPNEFLTEGASLTTQIDETTTSTDMVISVH
                     AQVETQAGSFSDCIQVRNTVKTNGIITFMEDSYYAFHVGLVQQVRTIPEGDGHVDGLE
                     FYFAGSDKTVHCTAGGLSSVLSDPEKENISSLTITGTMDARDFKTIRDEIPNLCYLNL
                     GDVEIAEYTGDQGPAPVFITYPANEIPRKSFYKLGEYNYYLYSVILPKNISSIGNLAF
                     EHCIALGQISFPPSLTSIGLQAFLGCTNLDKIIFEPPVSVAGIGKYAFGFCSKLESFE
                     IPESVTYIDTVAFLGSSASITVQENNPAYSAEGGVLFNKDKTALIYVPNTRAGDYEIP
                     SSVEVVKVDAFYNCRKLKSVTMPNTLTTLEIWAFENCSGLESISLPSSLNSIAGFAFY
                     NCTGLNSVYANAPIPPDLSESDSVFFNVDKNLCVLYVPAGSREAYQAADQWKDFLLIE
                     EFIPEQMMYYQADLDGSWQMHSLLADNPDDENSLAGWMYGTNSIDDDGQSVTANLVLN
                     GIDQPDFSSSVLVAAGGGVSVPGTDMDGFMEPGKQRIVITGTDGSGAFNFTVLQKTGH
                     ETDCEIADLEGTWQMHSLVTNDYGGSNNWAGWTHGVITIDDLGNTATSELIVNGESAA
                     GESSSLAISDEGIISSPENDIHGFLDADKQMAVLTMTDNSGGYSLSFLQKAVSDTIYE
                     LHNLKGAWRLHSLVTDNPSEPDDWSGWIHGLVTVDEKGESATTNIIANGESRPGFTSS
                     MSVSTSGIVTIAGTDMHGYMSADMQTIVLTGTDQGKGYNLSVMIRDYNSDATGIGETP
                     ATIPLFTAFPNPASEKVCIESTKGALIEIFNMQGELIKRTLTEGGITEINISSFPKGL
                     YFVRKKTPEGTFSCKLIRQ"
     gene            complement(11229..11675)
                     /locus_tag="PKI35_07655"
     CDS             complement(11229..11675)
                     /locus_tag="PKI35_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44291.1"
                     /translation="MKTLKRTATFLCLTAILFLVPAACEKNSDDDKEQTKTELLCREW
                     KVVSIGGLSANYECECDETLMSFKSDGKFSMIFKLDGAVIEDESFDGQWEWQVQDESV
                     TLEFQYFSEEEGWVTGFTILDIQQLTKDYLWFIFADDDEVYKCVPK"
     gene            complement(12171..12863)
                     /locus_tag="PKI35_07660"
     CDS             complement(12171..12863)
                     /locus_tag="PKI35_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX44292.1"
                     /translation="MINTTENNSMDTEKIKILLVDDSRIILDGLAFNFLLQHDFSVTG
                     KALSVQEALQSISAEIPDVVLLDISLQEEADGIDLLREMGQKYPAIKVLIFSQQKEVK
                     AIVDSVRAGARAYLSKDSSVEEIIGAVRMVAQGNGLFLGETIPPETLHHCFSGKPERK
                     NFTPYHLSDREIEIIRWLSKGYLSKEIAEIEHINVSTIESHKDNIKNKLGLKSIIEVV
                     VFCIKNGIIAAE"
     gene            complement(12847..17259)
                     /locus_tag="PKI35_07665"
     CDS             complement(12847..17259)
                     /locus_tag="PKI35_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNX44293.1"
                     /translation="MTKKPRLCFLLPLLLLFFLKAKAQSPDAFTDNYTKFVSYSANQG
                     LSNAFATDILQDKYGFIWIATRNGLNKFDGDGFIQYFHQPGNPRSLGDNFITSLAEDS
                     FGNLWIGTKNGLFRYDRPKNGFTQSGSDNAAPALSDKYIRALFADKEGNLWIDTRSGG
                     LDCLIISTNAVKHYPHTNPVFEGDYFYHFVYGDSKGRIWIGTRNFSPRILDKKTGSFQ
                     LVEGSNGNDGCSLFEDDNHIVWSAGNYGIFYFNEKTHHFADSSVYQIPFRVQATLNDH
                     EGNIWLGGAGDKILRFSQKDRKWTAFMHTETNLNSIISAEINKIFKDRQGNIWFATAN
                     GVSVIPKEINKFRHYSNIPGVNSLHSDKVTALLQDHKGILWIGTEDNGTDTVNFQREQ
                     FGNLQYRLLTRDLDRQTFKKEKEVLKQYARLGFITPESGISIDQDFSSYESFRNARLT
                     FSKKNENNISALYEDKEGSIWIGCWSGIGFNRFTCHDGFKRNSIFTRGLPPVFTNELW
                     CSNFYSDFLEDRNHNFWMATWEGVGLNLYNREKSQFEGKHFYGRGKSLDGRIYALHQQ
                     NDSILWLSPGLTLAAYLNTHTGRMRAFVNGKFPDDRLDEMKYLPFSGLEIAEIPFYES
                     ITGYVTDREGCTWVIPGRHEIFCYSSKSGRFKSYSLGNQFMEQISAATYSPDQHCIYL
                     SSVQGNLYAFSCISHQLRKIGPVPGTKSNQHTVVQGLRWFDKKVWIGLSNGLALYDPS
                     GNRFIYCMRLPGMAARAVKSVSSFAPAGNSLYFSSGNSIFVYDSGGKKFSDQSGKLNR
                     GSAISDIKALCYANNALWIGALNGLFKADLHSGKLTVFRHDESGPNTLPENEIQGLEL
                     VGDELFIATMKGLCKINTRTNRLTLLNQIPAGRLSSRLLTRLFEDKAGYLWIGTTESG
                     VNRLNLRTGMIDHFFELAYDTTSLLGNNVESIFQDHRGNIWIGTDKGLNRFNAAKMNF
                     FRYTALGKYNPASIKAIREDKSGRLWISTSNGLILFDAGSNAADHLCFLNGLQDNSFS
                     NASTRLSDGRLAFGGQNGVTVFDPSAIHPEKGFPQVILKNVRINDSLWFADFSEQTGL
                     SLKYYQDNISLDLATSEYFAPSLIHYRYRLLGFEKEWNYAGSSDKRVKYNNLKPGKYN
                     LEIGTTNTYGRWNPDTVHISIAVRRPWWVAWWFRGAAFVAVIACILAIIRIREASLKE
                     AGKKLEKIVTERTTELAETNKRLTESEQHLAETLAAKDKFFSIVAHDLKNPVLALRNI
                     SDRLVTEFENLTAEQQSELLSAMNNQIHLTYSFLDNLLLWALSQKNAVPFAPQACNLF
                     QLITRVTTLLDENARMKKISVNLNARDDGTVFADSNMLLTVLNNLLSNAIKFSYPGGV
                     ITIDTDSKAGFCEVSIKDQGTGIPGKQLDGLFRISSRYRTAGTAKESGTGTGLLLCKE
                     FVEKNGGKIWAESQEGKGSVFCFTIPEYQNDKHN"
     gene            complement(17368..18324)
                     /locus_tag="PKI35_07670"
     CDS             complement(17368..18324)
                     /locus_tag="PKI35_07670"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNX44294.1"
                     /translation="MKVIDHIKHAAGKTLFTFELLPPLKGETIESIYETADPLMEFNP
                     AFIDVTYHREEIVYKERADGLLERRTIRKRPGTVGISAALKFKYKIDVVPHIICGGFN
                     REETENALIDLYFLGIDNLLLIRGDNPAREKIFSPEPDGHAHTLDLVRQVVAMNRGKY
                     LEEELMNPIPTHFCIGVAGYPEKHEEAPNLTIDLQHLKEKVDAGAEYIVTQMFFDNAK
                     YFAFVDACRQIGIKVPVIPGLKPLSLKSQLTSLPKTFSIDLPEELVTEVLKCKDNQQV
                     RQAGIEWGIQQSRELIKAGAPVLHFFTMGKSDNIRKIVEAVF"
     gene            complement(18439..22104)
                     /gene="metH"
                     /locus_tag="PKI35_07675"
     CDS             complement(18439..22104)
                     /gene="metH"
                     /locus_tag="PKI35_07675"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNX44295.1"
                     /translation="MTTGKKDIFSELSHRVLVLDGAMGTMIQRHKLTESDYRGERFSH
                     HSVDLKGCNDLLCLTQPQIIDGIHRAYLEAGADIIETNTFNATSISLADYKLEAYVYE
                     INFAAARLAVNEADGFAEKTPGKQRFVAGSIGPTNKSASMSPVVSDPGFRAITYDQLA
                     EAYTEQVRGLIDGGVDLLLIETVFDTLNAKAALFAINTLFLEKGIRLPVMVSGTIADA
                     SGRTLSGQTAEAFLNSVSHTDLLSVGFNCSLGAEQLNPFIEELSRKAPCYVSAYPNAG
                     LPDQFGHYHETPAKMAKHISDLLVNGSVNIIGGCCGTTPDHIRELCRIAEEAKVRHIP
                     PKNHDLKLSGLEPLMVYEKSNFINIGERTNVSGSRKFARLIREEKYDEALSVARQQVD
                     AGAQALDVNMDDAMLDAEREMTRFLNLLMSEPEIAKVPVMIDSSKWSVIEAGLKCLQG
                     KAIVNSISLKEGEAIFKEHALKIRNYGAAMVVMAFDEQGQAATYERRIAVCERAYRIL
                     ADELGIPPEDIIFDPNVLAIATGIEEHNHYALDFLNATRWIKQNLPYARVSGGISNLS
                     FSFRGNDALREAMHSAFLFHAIKAGLDMGIVNAGNLPVYDDIPAGLLQHIEDVLFNRN
                     PDATERLISYTSQMRTSVRKESVAEAWRGLPVEDRISYAIIHGIDTFIATDVEEARLL
                     FTHAIGVIEGPLMNGMKAAGDLFEAGKMFLPQVVKSARVMKKAVSVLLSYLENERASA
                     GKISAGKILLATVKGDVHDIGKNIVSVVLGCNNYKVIDLGVMVNAEKIIETAVREKVD
                     IVGLSGLITPSLDEMAFVASEMQRRGLKVPLLIGGATTSEMHTAVKITPQYQYPVVHV
                     RDASRVAGVAASLLSAGMKNDFLASVNEKYSRLREKHKDAMAGISYLTLEQARDNRFK
                     ASWKDYKPFKPSFTGVKVLENYPLGEISRYIDWTYFLHAWNISGKFPDIFENPVKGGE
                     ARKLYDDAQVLLQKMIEDKMVRANGAFGFFPAVAVGDSIELTLGDDTKLRLPFLRNQE
                     QKEGNVPNLSLADFIVPAGTGKTDFIGLFAVTAGLGIEKWVQYFKERHDDYNAIMLKI
                     LADRLAEAFAELLHLKVRKEYWGYAAEENLTTEEVLRQGYRGIRPAPGYPACPDHSGK
                     RVIFDLLGAERSGMHLTENFAMYPAASVSGYYFAHPQAKYFNVGRISRDQVAGYALQK
                     GETVESVERLLATNLNY"
     regulatory      complement(22223..22331)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         5727 a         5584 c         5120 g         6040 t
ORIGIN      
        1 ggtctgctac gccggcaggc cttctccctg agcgcctgtc ccgattcatc gggaagaagg
       61 cgccggaggc ggatgaggcg atcataaaaa aaagcaacca tacgtcgtat gattgctttg
      121 cgagccactc aggggactcg aacccccgac ccgcgcatta cgaatgcgct gctctaccaa
      181 ctgagctaaa gtggcaaaaa aaacctgcag gcaacatcca cgcaggttat tttggtcggg
      241 atgactggat tcgaaccagc gacctcttcg tcccgaacga agcacgctac caggctgcgc
      301 tacatcccga aataaattta agaacctggt aattctttca actttcagcg gtgcaaagat
      361 aacataaaat aatttaacac gaaaaatccc tgctccgata aaatgatttg caccctctat
      421 ttcatatacc tgtttatccc ggaaataatc tgttcgagct taaatggttt gctgataaaa
      481 tcggagaatc cgcatgccat gatcctttta atgtcgttcg ggtcggcata ggcagtttgg
      541 gccaggacag gcagttgcgg cctgaattcc ttgataagca tggttgcttc gcaaccatcc
      601 atttccggca ttttaatatc catcagcacc atatcgatct gtgggttcaa tctgcactgt
      661 tcaacggcga tggcgccatt gaccgccctt atgatcttta ccggcgacgc tgataacatt
      721 tcttccagca atgcaaaatt cgtgtcttcg tcttcagcaa ttagaattgt tttcaggccg
      781 tcagcttcaa tttttaaagc gctcaccggc tgttctttag gaatagcttc cctgagtgaa
      841 ctgatatatg gcaatgtaaa gcagaaaacc gaaccaattc ccggttcgga atcgacccgg
      901 atttcgcccc ccagcaaatc ggcataagct ttcgaaattg acaatcccag ccctgtgcct
      961 ccataccggc ggcttacgtt agtctcaacc tggcggaacc gttcaaatat tttgtcatgc
     1021 atatccgaag gaattcctat cccggtatct ttaacaaaga attcaagcat cccggctttc
     1081 agattatacc cgaacttgat ataacccttt tcggtaaact ttaccgcgtt gctgagaaag
     1141 ttgcccagaa tggccgtcag tttggcctgg tcggtaacaa taactgcctc ctgatcggga
     1201 agcgcagttt tacaatgcaa taaaatattt ttccgttggg caagaacgcg aaattcctca
     1261 tagagtactt tgatggtgtc attaacattt acatcttttt cgtttaccct ggcttgccct
     1321 gcttctacag tggctatatt cacaatatcc gttatgatgg ccagcaactg gttgcttccc
     1381 tgcagcatca gatcggtgta ttgcctccgt ttttccgcta tgatttcagt atcattgagc
     1441 aagtcggcaa agcctacaat ggcattcatg ggagtgcgga tttcatgcga gatattctgc
     1501 aggaaggcgg ttttcagccg atcgctttct tcagctttat ccttggcttt gatcagatcg
     1561 tccagcatcc ttttcttttc cgtaatgtca tccatgatca ggatgtagtt gacaatggtc
     1621 ccgttggcat ccagtatcgg agaaatgatc agattttccc agatgatggt atgatccttt
     1681 ttccggttca taaattcgct ccgccagatt ttacctgacc ggagagtcct ccacatggcc
     1741 gaaaattctt cttccgggat atggcccgga ttgaagatcc ttgggtttct ccctttcaca
     1801 tcctccattg aatattgcat gattgacgaa aactggtcgt tgacaaattc aatcttcccg
     1861 tcagcattgg taatgacaat ggaagaaggg ctttgctgga ttgctttgct cagtgtcctg
     1921 atcagttttt cggctttcat gctctctgtg atatcgcggt cgatgccgat ggaaaccgat
     1981 ttcccgtgcc aattgacagg taagccgata tattcaatga tgttttcttt cccgtcgggg
     2041 cggagccatg aaaacagtcc tttataggct tcagaagggc cctgggggcc tttcccggaa
     2101 atgcccgtcg ccttttcaac cggaacctga aggggcagcg tattgccaaa ctccaggagc
     2161 tccgcgcggc tgcgacccga catctcagca taccgttcat tgcagtccac cagctttttt
     2221 tccgatcgtt cagtaccttc ctcataaatg ctgatgccat cgaatacatt ttcgaatacc
     2281 atccggaacc ggtcctccga ctccctgatt gcctcctcgg cgctttttcg ctcagtgata
     2341 tcgttgaatg tagccatgat gcataccgga ctgccatcgg gagaaattac gaaattgacg
     2401 gaaaactgga tatcaatcgg cgtcatatcc tttttaaagg cctttttctc gcccgaataa
     2461 acgccttttg cctttacttc ggccaatatt tcttctattt gctccctgta ttggtcatca
     2521 tcatatattt cttttcctgc gacttcctcc ctgttcttaa tgccccagag attataatag
     2581 gcgtcgttgg cataaattaa tctgccctta agatcggata gccctattgc gcttgaagaa
     2641 gactcgatag caaagtcctt gatgagcaac tgttcttcgg tacgcttgcg ttcggtattg
     2701 tccagggcca tggtcagtat gcagtcctca ccttttacct ttatgtatac cgccgacatc
     2761 agcaaagaaa tgaaatcgcc gcccttcgtc cgcatctcta tttcaaatcc cctggctatt
     2821 tcacggtcat gcaataaatc aagcattgcc tgtcgcctgg acttatcagc gtataatccc
     2881 agttcaaccg gagaatggcc gatgacttcc tgtgactgat agcctgtaat ctccagccat
     2941 gcattgttaa cgctcacaaa ggtgttatct ttcagatttg tgatagcgat agggataggg
     3001 ctggaattga aaaccgttga taacaaagcc tcgcttgcaa tcagggcttc ttccgcctgt
     3061 tttcgtacgg taatgtcttt catgatgccc aggtagcgac aggaaagatt aaccggcgat
     3121 tggatgagat tggattcttc ttcgatccag caatactcgc cgttcttttt ctgatacctg
     3181 tattgcgcct taaagggaag atggctctcc agagatttgt tgaattcaag aagcgttttg
     3241 gtcctgtcct ccggatgaac catcccgctc cattgctcaa tgccgaaatt ttcgtactca
     3301 gcaggcgtat aaccggttat ttgctcaata gccccatccc atttcacttt tcctgtctcc
     3361 acttccaaat cataaaccag gcttttcgac aatatggcaa ctgtccggaa acgttcatcg
     3421 ctttcgcgca gtatctcctc ggcctttttc ttttcggtaa tgtcttcaac cagggaatat
     3481 acagatatca gcgcgccttc cggatcatac aatactgaat tgtgccacac acaggtaata
     3541 acagaaccat ctttccgata gttgcgatgc gtatgcgaat aaccggtcct ttggcctgcg
     3601 atcatttcgg cagaaaagtc ggttacattt gtaacatctt cctcataaat ccagtgaagt
     3661 tcatgataac ttttccccat tatttcatca gcctgccaac cgaacatttt cgctgcattt
     3721 cccgaccaga aaacgacctg gttattggcg ttgaattcca caaccgcaag aggggtgttt
     3781 tcaatgtaaa attttagttt ttgcagggtc acctgcaacg attcctcggc ctgccggcgt
     3841 ttggtaatgt cgtttactat ggcaaaaaat atatcctgat tctcctgctt atgaaactgt
     3901 atagttactt ccacaggata ttccgtacca ttcttcctcc ggtgcacagt attcacgaac
     3961 accgcaattt ctttaccttc tgccagttgc tcaaacaatt ctttgaggtc cgtttccgag
     4021 tatcttgcat tcagactgaa cggggtaagt tgttgtattt cctccagcgt gtaaccggta
     4081 ttcaggagtg cacgctgatt ggcatacaga aatttaagcg aatcagaatc tatgacaaag
     4141 atttcattca gactgtgttc tatgatatcc aatagttgca ggcgtttgct ttcaaccctc
     4201 ctgaattcgg taacgtcgtt tatggatgac aggatacatt cttcattatt cagcgtgatt
     4261 acttctcccg aataatcacc aatcatcaga tttctgtttt taataaggaa atgaaatcct
     4321 ttctgctggg cttttccatt cttcagcaat tccccaacca gccaggacct gtcttcctgc
     4381 ctgttccaca gattaatatc aaccgtggtt ttcccgataa cttcggagta taaataaccg
     4441 ctcgttttca ggaagccatc attaacctca aggattttac cgtcgctcag ccttgtcagc
     4501 aatatacaat agggagacga attgaaaatt tttgaatatt ttctttcttc catgctgata
     4561 ttcaacagca aacgctggtt gagcatcagg gcaagatagt atatcaatat caggcttaat
     4621 aactgatagc taatcactat agttacctcg agtatacccg gatataagaa atttgtcgct
     4681 ccgggtttaa aaaaaacaac ttccagtatt ctgacaacat ttatcacact gaagaacgcg
     4741 aataccaacc cgatcccaag catcagcttt cgtttttcag cggattttaa acgaaatact
     4801 atccaggcgc cttgggaaca gaagaccacc aatgccccgg ctaaattgat ggtacgaatg
     4861 gcaaggtcgt gatggatgaa ggtataccag gcagtaataa gggtatagat ccccaggatg
     4921 atatagttat gaatttgacg gccccggtat tctgcgaaac tctgcaagcc tgccagcccc
     4981 agataagcgc ctaggatcat caggatattg gaaacaaaga tcgatagcca gccggggatc
     5041 aatccccgca aggctatcag gataaaactc accgtttgca gggcaaacag cattacccag
     5101 aagaaagtac cggggaaacg gttgcggttc tgctgccaca atgctgttgc aacacctgtg
     5161 gaaacgatac ttgtaagcat ataacaaaca agcaccgtgc gcatatcaag tattcccata
     5221 caaacaatag ttaataaacc tgccgctagt gactaattca cactccatta gctttaaaat
     5281 ccccagatat caacaacgtc cggacctgaa aagcagttga agcactaaat tacaaaatca
     5341 ggtttagccc tcaaaataaa tttcacggct gaaaactgct ccaaggcagc aagccgtccg
     5401 gagcagggtg ccggttcccg gacaataaga tcatcacgcc cgcagggata gatgaacctt
     5461 cacatacgta ttgtaggtct gccctgaaaa ttctgcaccc ggaacatgac ggcaggaaat
     5521 gcgtcagaaa attataaaag caaaaccctc tgttatcgga tggataaaca gagggtttat
     5581 tggttgaatt gaagtctccc cgcggggact cgaaccccgg gcccattgat taagagtcaa
     5641 ttgctctacc agctgagcta cggagagatt tcgggctgca aaaatagtaa ttttttcaat
     5701 cggcagcttt tcagccgggg ttatttttgt ctttatagtc ccggtttcgt tatcctcaat
     5761 atctttggga aaaaatcgcc catgaaaaga accgcatttc tggcagctat tcttttaatc
     5821 agcatgactg cggctgagtc ccagaccgtg aataaaaact acatggcaaa cgaagtaaag
     5881 aaggcttttt cgcatgcctg ggaagggtac aaacaatatg cctggggtta tgatgccctt
     5941 aagcccctga gcaggcaagg ccataactgg taccagcaat ccctgctgat gacgccggtt
     6001 gatgcctatg atgtgatgaa gatcatgggc cttgaaaaag aagctgcgga agccaaacag
     6061 cttatctttg ggaagctgaa cttcgacgca gatatggaag tccagaattt cgaagtttcc
     6121 atccgcatcc tgggcgggtt gctttccgct tatgaactcg acggcgatac cggattcctg
     6181 aacctggcaa aagaccttgc cgacaggctt atagttgttt tcaactcccc taccggcatg
     6241 ccgtaccggt atgtgaacct taaaaccgga aaagtgagcg gaccggtatc gaatcctgcc
     6301 gaaataggga cttatatcat agaatacggc attctgagcc atcataccgg tgatccaaaa
     6361 tattacgaaa cggccaaaaa agcaatggtg aaactgcata gcctccgatc ggcaatcggc
     6421 ttgcccggcg caggaatcaa cgttgaaacc ggagaatgga ccaataccga aagttccatt
     6481 tcaggaggca tcgattcata cttcgagtat atgctgaaag gagccatcct tttcagggac
     6541 aaggatttgc gcgaaatgtg ggaaaccagc gccgaagccg tcaaccggta catggccgat
     6601 actgtatcgg gcaacctgtg gtacggccgg gttgatatga acaccggcaa gaggacccgc
     6661 actatttacg gggcattgga tgcctttttt gccggttccc tggcgcttga cggcgatctg
     6721 caaagggccg ccatgttgca ggaatcgaat tttaagatgt ggatgttcac cggaatcgaa
     6781 cccgaagtgc tcgattattc gaagatggaa atcaggtacc caggatacgt cctgcgcccc
     6841 gaaaacgtgg aaagcgccta ttacctatac cacttcacgg gtgatgaaaa gtacctgagg
     6901 atgggaaaag agatgtttga taacctggtg aaatattgcc gttcggagac gggtttcacg
     6961 gcgctgaaaa acgtgatgac caaagagcgc gatgacgata tggaaagttt tttctttgcc
     7021 gaaacgctga agtacttttt cctgctcttt gatgatggcc gctcactcga tttcgacaag
     7081 gtgattttca acacggaggc gcatccctac aagcggttct gaaaaatccg gctgacaaga
     7141 aaaggaatgg aatacgcttt cttcctgatt ttattgcctg ccgtgcacgg ctgaagataa
     7201 ttcgccgagc aatccggcat ctttttccag cgccgtacca acgacaatca gatcagcgcc
     7261 cgccttgcaa atttccgatg cagtttcagg cgttcttatt cccccgccga cgatgagcgg
     7321 tatgccgatg ctttctttca cctgtttcac cataccggct gttacaggcc gttgggcgcc
     7381 gcttccggct tccaggtaaa tcagtttcag ccctaacatt tcaccggcca gggctgtaca
     7441 gatggcgata tcatttttat cggccgggat gggcgcagta ttgctcatat actgaacagc
     7501 cgtctgcctg ccgctttcga tgagcatata tccgcatgga attacctcta acgggctgac
     7561 cttcaggtaa ggggccgata tcacatgccg gccgatgagc atttcggcat tccgcccgct
     7621 gataagcgac aggaaaagga tagcgtcagc tttccagctc aattgcattg tgttcccggg
     7681 gaataacacc accggcacat ttgcattttc tttgatggtt tttatacagg catcgagctg
     7741 attattgacc agcaggctgc cgccgacaaa aaagtagtcg gcccccgtat cgcctgctct
     7801 tgaggcaagt gcagcgactt ccttcagcga cagcttgtcg ggatcaacca gtacggcaag
     7861 ctgttttctg ccttttacgg ctttatcgag gatcgtattg taaaccggca tacgcaggga
     7921 tatgtttaca aaggtatgca aaagagatca ctgctgataa aaagtaaacc cggtctgaaa
     7981 acaggccggg aatacgcgtt cgtaatagta aaaactcagt aatatctaaa atgttgatat
     8041 aaaaccatgg agcaatcagg ccatcgcgac aaggtgattt gccgtttccg gcttacatat
     8101 atgataacgc cattattgcc tgatcaattt gcagctaaag gttccttccg gcgttttctt
     8161 tctgacgaag tacagtcctt tcggaaatga agaaatattg atttctgtaa ttcctccttc
     8221 cgttagagtt ctttttataa gttctccctg catgttaaag atttcaataa gagcgccttt
     8281 tgtgctctcg atgcagactt tttcgcttgc cgggtttgga aatgctgtaa acaacggaat
     8341 cgtggccggc gtttccccga tgcctgttgc atccgaatta taatccctta tcattaccga
     8401 aaggttgtat cccttgccct gatcagtacc ggttaacaca atggtttgca tatcggcgct
     8461 catataacca tgcatatctg taccggcaat cgtaactatt cccgaggtgc tgaccgacat
     8521 agaagaagtg aaaccgggac ggctttcacc gttcgcgatg atgttggtgg tagcgctttc
     8581 tcccttttcg tcaaccgtca caaggccatg tatccagcct gaccagtcat caggttcgga
     8641 agggttgtcg gtaaccagcg aatgcaaccg ccaggcccct ttaaggttat gaagttcgta
     8701 tatcgtatcg gaaaccgcct tttgcaaaaa ggaaaggcta tatccgccac tgttgtcggt
     8761 catggtaagc accgccatct gtttatccgc atccaggaat ccgtgtatat cgttttccgg
     8821 agaactgata attccctcat cgcttattgc cagtgatgag gattcgcccg cagcagattc
     8881 gccattcact atcagctccg atgttgccgt attccccaga tcatcaatgg tgatgacgcc
     8941 atgcgtccag ccggcccagt tatttgaccc gccgtaatcg ttggtcacca gtgaatgcat
     9001 ctgccaggtt ccttctaaat cagcaatttc acagtcagtt tcatggccgg tcttttgcag
     9061 taccgtgaaa ttaaaggcgc cgctgccatc cgtgccggta ataacgatcc tctgtttacc
     9121 gggttccatg aaaccgtcca tatctgttcc gggaaccgaa accccgccgc cggccgcaac
     9181 caggaccgag gatgaaaaat caggctggtc aatgccgttg agaaccaggt ttgccgtgac
     9241 actctgacca tcatcatcaa tcgaattggt gccatacatc catccggcca ggctgttttc
     9301 atcatccgga ttatcggcaa gcagggaatg catctgccac gaaccgtcca gatcggcctg
     9361 gtaatacatc atttgttcgg ggatgaattc ttcgatcagc aggaaatcct tccattggtc
     9421 cgctgcctgg taagcttctc tggaaccggc cggcacataa agaacgcaga gatttttatc
     9481 aacattaaag aaaacgctgt cgctttcgga caaatccggg ggaataggcg cattcgcgta
     9541 tacggaattc aggccggtgc aattataaaa tgcgaaaccg gcgatggaat tcagagagga
     9601 aggcagcgat attgattcca ggcctgaaca gttttcaaaa gcccatatct ccaaagttgt
     9661 cagggtattg ggcatggtaa cggatttgag tttgcggcaa ttataaaagg catccacctt
     9721 gacaacctca accgacgaag gtatttcgta atcgccggcc ctggtattcg gcacatatat
     9781 gagcgctgtc ttgtctttgt tgaaaaggac gcccccttcg gccgagtaag cggggttgtt
     9841 ctcctgcacg gtaatcgacg ccgaactccc caggaatgca acggtatcaa tataggtgac
     9901 tgattcagga atttcgaatg actcaagctt ggaacaaaaa ccaaacgcat atttgcctat
     9961 gccggcaact gatactggag gctcaaagat gattttgtcc aggttagtac aacccaaaaa
    10021 agcctggagc ccgatacttg tcaacgacgg aggaaaagaa atttgaccaa gggcgataca
    10081 atgttcaaat gcgagattcc ctattgaaga gatatttttc ggcaaaatca ccgaataaag
    10141 gtaatagttg tactcgccca atttataaaa tgattttcgg ggaatttcgt ttgccggata
    10201 ggtgataaat acaggagccg gcccctgatc gccggtatat tctgcgattt ccacgtcgcc
    10261 gagattgagg taacacaggt tcggaatctc atcacggatg gtcttaaaat cccttgcatc
    10321 catggtcccg gtaatcgtga ggctggaaat gttctccttt tccggatcgg agaggacgga
    10381 tgaaagcccg ccggccgtgc aatgcactgt tttatcagaa ccggcaaaat agaactcgag
    10441 accatcgaca tgcccatcgc cttcggggat cgttcttacc tgttgcacca atccgacatg
    10501 aaaagcataa tagctgtctt ccatgaaagt aatgattccg tttgttttca cggtattccg
    10561 aacctggata caatctgaaa atgatccggc ttgtgtttca acctgggcat gaacactgat
    10621 caccatatca gtactggtag ttgtttcgtc gatttgagta gtcagggacg cgccttccgt
    10681 caggaattca ttcggaaaaa tgatcacggg agttgaaggg atgacggcgt ttaccaggct
    10741 gggtgaagcg ccgttatcat cgccggccat actgtatgcc cctgccacga tattcccttc
    10801 ctggtcgggt ctgatccaga gatacctgaa atacaaatcc ccggtgcaat tccccgttgt
    10861 atagttgcct tcccaaccct gttccacaaa ataatccacc ccgtttactt cttccatcca
    10921 gcggtattta tactcggtat tccggaaaca ccagctcccg cttgcagaac ttgtttgcaa
    10981 catggtccgg ttgcctactt taaaaggcag gtagtaatcg gctgcggtct gcccggctga
    11041 agataatact gccaggaata aggccaggaa cagaagatta attttcatct gagtgattca
    11101 tttattaagc actagtactt catcattttt aagggtaaca gaaagatcac ggataaacgc
    11161 taatggcgtt tgatccgatt tataaaatct atcaaaactc accctggcaa cccggattgt
    11221 tacctaaatt atttaggcac acatttataa acttcgtcat cgtctgcaaa tatgaaccag
    11281 agatagtctt tagtaagttg ctgaatatct aatattgtaa aacctgtaac ccatccctct
    11341 tcctcgctga aatactgaaa ttccaacgta acagattcgt cctgtacctg ccattcccat
    11401 tgcccatcaa aagactcgtc ttcaatgacg gcaccatcca acttgaaaat catgctgaat
    11461 ttgccgtcgg atttaaaact catgagggtt tcatcacatt cgcattcata attggcgctt
    11521 agtccgccga tggatacgac tttccattca cggcaaagca attccgtttt tgtctgttcc
    11581 ttgtcgtcat cgctgttttt ttcacaggcc gcagggacca gaaacagaat ggccgtcagg
    11641 caaagaaagg ttgcagtacg ttttaaagtt ttcatagttt ttgtttttca gttgaatgaa
    11701 tttgtaacta tccattcatt tggtttgttt ggcaaaaata tgttctgcca aacgctattt
    11761 caatcctcat ttctgaggat ctgtatcctg atttttgagg gtttcagagt gaatccctac
    11821 aacccgaaaa tgaagaaaac gaggatggct gcaagtaaac ccgaaagggc cgctaccagc
    11881 agattgccgg attcttcagg cctgtttttc ctgtatgtta aaaaccaaat aatacctgcc
    11941 ggaggaagca ggaacgaaat agtaagcctt aaaacgcgaa acaacctctt ccggctgcat
    12001 ggacgtaaaa cacaattcat aaaacaggat ccgttacctg ccacaaaatt attcccgggc
    12061 tccgggattg gtcaatagtg aaaatccact agaggaagtg gtaattttca ggtagcaggg
    12121 agtttcaggt cccggaaccc tcacggaaaa caaatcatgg caaaggccgg ttattcagca
    12181 gcgataatcc cgtttttaat gcaaaacact acgacttcta tgattgattt aaggcccagt
    12241 ttattcttga tgttatcctt gtgcgattca atggtgctta catttatgtg ttctatttct
    12301 gcaatttctt tcgataaata ccctttcgaa agccacctga tgatctctat ttcccggtcc
    12361 gacagatggt aaggcgtaaa attctttcgt tcgggcttgc ctgaaaaaca atgatgcagc
    12421 gtctccggcg ggattgtttc acccaggaac agcccgttgc cttgggcaac catcctgacg
    12481 gcgccgatga tttcttccac ggatgaatct ttggataagt aagcccgggc gcccgccctg
    12541 accgaatcga cgatagcctt tacctctttc tgctgggaga agatcagcac ttttatcgcg
    12601 gggtacttct gccccatttc cctaagcaaa tcaatgccgt cggcctcttc ctgcaatgag
    12661 atatcgagca aaaccacatc gggaatttca gcggaaatgc tttggagagc ttcctgcacc
    12721 gaaagggctt tcccggtaac ggaaaaatca tgctgaagca agaaattaaa agccagcccg
    12781 tccaggatga tccgggaatc gtcgaccagc agaattttga tcttttcggt atccatcgaa
    12841 ttattttcag ttgtgtttat cattctgata ttcaggaatc gtgaaacaga aaacgctccc
    12901 tttcccttcc tggctttcgg cccaaatttt tcccccgttt ttctcgacaa attctttgca
    12961 cagcagtaag ccagtgcccg taccgctttc ttttgccgta ccggccgtcc ggtacctgga
    13021 tgaaatcctg aacagtccgt ccaactgctt gccgggaata ccggttccct ggtctttaat
    13081 gcttacctcg caaaaaccgg ccttgctatc cgtatcaata gtgatgactc ccccgggata
    13141 ggaaaattta atggcattcg acaggagatt gttcagaacc gtcagcagca tgttactgtc
    13201 ggcaaacaca gtaccatcat ccctggcgtt aagattaacc gagatttttt tcatccgcgc
    13261 attttcatca agcagggtag tcacccgggt gataagttgg aacaggttgc aggcctgcgg
    13321 tgcgaaagga acagcatttt tctgcgacag cgcccacaac aggagattat cgaggaatga
    13381 gtatgtaagg tgtatctggt tgttcattgc cgaaagcaat tctgactgtt gttcagcggt
    13441 gaggttttca aattcggtta caaggcggtc ggagatattt cttagcgcta acaccggatt
    13501 tttaaggtcg tgagccacaa tcgagaagaa tttatccttg gcggccagcg tttcggcaag
    13561 gtgctgctcc gattcggtaa ggcgtttgtt ggtttcggca agttcagtcg tacgctctgt
    13621 aacgattttt tcaagttttt tcccggcttc cttcaggctt gcctccctga tccggatgat
    13681 agcaagaatg caggcaataa ccgccacgaa cgccgccccg cggaaccacc aagccaccca
    13741 ccaggggcgg cggacggcaa ttgatatgtg tacggtatca ggattccatc tgccgtatgt
    13801 attggtcgtg ccgatttcaa ggttgtattt tccgggtttc aggttattgt acttcacccg
    13861 tttatccgaa cttcccgcat agttccattc tttttcaaat cccagaagtc ggtaacggta
    13921 atgaataagc gaaggcgcaa aatattccga tgttgccaga tcaagtgaaa tgttatcctg
    13981 ataatacttc agcgaaagac ctgtttgttc cgaaaaatca gcaaaccaaa gggagtcgtt
    14041 gattctgaca tttttaagga tcacctgggg aaaacctttt tcaggatgaa tggcagaagg
    14101 atcaaaaact gtaacgccgt tttgcccgcc aaaagccagc cggccatcgc tgagccttgt
    14161 tgacgcattg gaaaacgaat tgtcctgtaa accgtttaaa aaacataaat ggtcagccgc
    14221 attgcttccg gcatcaaaca aaatcaggcc gttggaagtg cttatccata accggcccga
    14281 tttatcttcc cggatggctt tgatagaagc cggattgtac ttccccagcg ccgtatagcg
    14341 gaaaaagttc atttttgccg cattgaaccg gtttaaccct ttatcggtac ctatccagat
    14401 attgcctcta tgatcctgga atatgctttc aacattattg cccagaagac tggtggtatc
    14461 gtatgccaac tcaaaaaaat ggtcaatcat gccggtgcga aggtttaacc ggttaacacc
    14521 tgattctgtg gtgccgatcc acaagtagcc cgccttgtct tcaaacagcc gggtaagcag
    14581 cctggacgat agccggccgg caggtatctg attcaaaagg gtcaaccggt ttgtcctggt
    14641 attaatttta caaagtcctt tcatggtcgc gatgaataat tcgtcgccca ccagctccag
    14701 gccttgtatt tcattctccg gcaatgtatt tggcccggat tcgtcatgcc tgaaaacagt
    14761 aagcttcccg gaatgcagat cagctttgaa aagaccgttc agcgcgccga tccacagggc
    14821 gttgttcgcg taacacaaag ctttgatatc gcttatcgca gaaccccgat tcaatttccc
    14881 gctttgatcg ctgaacttct tccccccgct gtcatataca aaaatgctgt tccctgagct
    14941 gaaatacagg ctgttaccgg caggggcaaa gctgctcacg ctctttactg cgcgggcagc
    15001 catcccagga agtcgcatgc agtatataaa gcggtttccg gacggatcgt acagtgcaag
    15061 gccattgctg aggcctatcc agaccttttt gtcgaaccat ctcaggcctt gaacaacagt
    15121 atgctgattg gatttcgttc cggggaccgg ccctattttt ctcaactgat gcgagatgca
    15181 actaaacgcg tacagatttc cctgtaccga acttagataa atacagtgct gatccggcga
    15241 ataagttgcc gcgctgattt gctccatgaa ctgatttcca agagaataag atttaaacct
    15301 gccgcttttt gaagaatagc agaaaatttc atggcgacca gggattaccc aggtacagcc
    15361 ctccctgtca gtaacataac cggtaatgct ttcataaaac gggatttctg caatttcaag
    15421 gcctgaaaaa ggcaggtatt tcatttcatc aagccggtcg tcaggaaatt taccgttaac
    15481 aaacgccctc attctgccgg tatgggtatt cagatatgct gccagcgtca gacccggtga
    15541 aagccagagt atcgaatcgt tttgttgatg caatgcatat atccttccgt cgaggctctt
    15601 tccccggcca taaaaatgtt tgccttcaaa ctgcgatttt tcacggttgt acaggttcag
    15661 cccgacgcct tcccaggtag ccatccagaa attatgattc cggtcttcaa gaaagtcgga
    15721 atagaaattg gaacaccaca gctcgtttgt gaaaacaggg ggtaatcccc gtgtaaatat
    15781 cgaattgcgt ttgaaaccgt catggcaggt aaaccggttg aacccgatcc cgctccagca
    15841 accgatccag attgagcctt ccttatcttc atataacgcg ctgatattgt tttcgttctt
    15901 ttttgagaag gtcaggcgtg cattcctgaa tgattcatac gaactgaagt cctgatcaat
    15961 acttataccg gattcaggtg tgataaaacc gagacgggca tactgtttaa gcacttcctt
    16021 ctctttttta aaagtctgcc ggtcgaggtc ccgggtaaga agacgatatt gcagattccc
    16081 gaattgttcc ctttggaaat tgacggtatc cgttccgttg tcttcggtcc cgatccataa
    16141 aatacctttg tgatcctgaa gcagagccgt caccttatcc gagtgaaggc tgtttacacc
    16201 gggtatgttg ctgtaatgcc tgaatttgtt gatctccttt ggaatcacac ttacgccgtt
    16261 cgctgttgca aaccagatat ttccctgacg gtctttgaaa attttattga tttcggcaga
    16321 aatgatggaa ttcaggttgg tttctgtatg catgaaagct gtccatttgc ggtctttctg
    16381 actgaaacgg agaattttgt cccccgcccc gcctaaccag atattgcctt cgtgatcgtt
    16441 cagtgttgct tgcaccctga aagggatttg atatacggat gaatcggcaa aatgatgcgt
    16501 cttctcatta aaataaaaaa ttccataatt gcctgcgctc cagacgatat ggttatcgtc
    16561 ttcaaaaagg ctgcaaccgt cattaccgtt ggagccctca acgagttgaa aactgcctgt
    16621 ctttttatcc aggatacgag gggaaaaatt ccgggttccg atccatatcc tgccttttga
    16681 atccccataa acgaaatggt agaaatagtc cccttcgaaa accggatttg tatgcggata
    16741 atgttttaca gcgtttgttg aaatgatgag gcaatcaagt ccgccgctcc ttgtatcgat
    16801 ccataaattc ccttccttat cggcaaagag cgctctgata tatttgtctg aaagagcggg
    16861 agccgcgtta tccgatcctg actgagtaaa accgtttttc ggcctgtcat acctgaataa
    16921 cccgtttttc gttccgatcc agaggttgcc aaaactatct tccgccagcg aagtgataaa
    16981 gttatcgccc agggaccggg gatttcccgg ttgatggaag tattgaatga aaccatcccc
    17041 gtcaaactta ttcagtccat tcctggtggc tatccagata aaaccatatt tatcctgaag
    17101 gatatctgtc gcaaaagcat tgctcaagcc ctgattggca gaataggata caaattttgt
    17161 gtaattatcg gtaaaggcat cgggcgactg cgcctttgct tttaaaaaaa acaacaggag
    17221 taacggcagc aagaaacaca ggcgaggttt cttagtcata cgcggttaaa atacaccctt
    17281 aaaaatagtt acaatagcaa agatatgaag gtgcccgata atttttcgaa aaataatgaa
    17341 tgatttattc cggctccaaa cccaacttta aaatactgct tcaacgattt tgcggatatt
    17401 atcgcttttc cccatcgtga aaaaatggag tacaggagca ccggctttaa tcagttcgcg
    17461 gctttgctgt atcccccatt cgatgcctgc ctgccgtacc tgctggttat ccttgcattt
    17521 taatacttcg gtgacaagtt cttcgggcag gtcaatggag aaagtcttcg gaagggacgt
    17581 caattgagac ttcagcgaaa gcggcttcaa accggggatg accggtacct tgataccaat
    17641 ctgccggcag gcatccacga aagcaaagta cttagcattg tcgaaaaaca tctgtgtgac
    17701 gatatattcc gctccggcat ccactttctc cttcaggtgc tgaaggtcga tggtaaggtt
    17761 tggcgcttcc tcgtgttttt ccgggtaacc ggcaacaccg atgcaaaaat gggtaggtat
    17821 agggttcatc agttcctctt ccaggtattt accccggttc atggcaacca cctgccttac
    17881 cagatcgagg gtatgggcat gcccgtcggg ttcagggctg aaaatctttt cccttgccgg
    17941 attatcgcct ctgatcagca acaggttatc gatgccgaga aaataaaggt cgatgagggc
    18001 gttctccgtt tcttcgcggt tgaagccgcc gcagatgata tggggaacca catcaatctt
    18061 atacttgaat ttcaaggctg ccgaaatgcc gaccgttccg ggacgtttgc ggattgtgcg
    18121 tctctccaga agcccgtcgg cacgttcctt gtacacaatt tcttcacgat ggtaggtaac
    18181 atcgataaaa gccgggttga attccattaa cggatcggcc gtttcataaa tcgattcaat
    18241 ggtttctcct ttcagaggag gcaggagctc aaaagtaaaa agagttttac cggcagcatg
    18301 ttttatatgg tcaatgactt tcatggatag ttcgtttttt tgacaggttc ccggctggac
    18361 ggtaagtcgc cgggcaatca ggctgatctg gtttgaaagc ctgatcagcc tgattcaact
    18421 tctattcaat attcccggtc aataattcag gttcgttgcc agcagtcttt ccaccgattc
    18481 aaccgtttcc cctttttgca gggcataacc ggccacctgg tcgcggctga tgcggcccac
    18541 attgaaatac ttagcctgag gatgggcaaa atagtacccg ctgaccgaag ctgccggata
    18601 catggcaaag ttttcggtaa ggtgcatccc cgacctttcg gcccccagca aatcgaaaat
    18661 aacacgcttc ccgctgtggt cggggcatgc agggtaaccg ggagccgggc gtatgccccg
    18721 gtatccttgt cgcaggactt cttctgtcgt aaggttttct tcagcggcat aaccccagta
    18781 ttcctttcgc actttaaggt gcagcaattc ggcgaaggct tcggcaaggc ggtcggcaag
    18841 gattttgagc atgatcgcat tgtaatcgtc gtgccgttct ttgaaatatt gcacccactt
    18901 ttcgattccc agcccggcag ttacggcaaa caaaccgata aagtcggtct tgcctgtgcc
    18961 tgccggaact ataaaatcgg ccagtgagag gttggggaca tttccttcct tttgttcctg
    19021 gtttcttaga aaaggaagcc gcagctttgt atcgtctccc agggtcagtt cgatactgtc
    19081 gccgacggca actgccggga aaaaaccgaa agcgccgttt gccctcacca ttttatcttc
    19141 aatcatcttt tgcaacaata cctgggcatc gtcatacaat ttacgggctt cgccgccttt
    19201 taccgggttc tcaaaaatgt ccgggaattt cccgctgatg ttccatgcat gcaagaagta
    19261 tgtccagtcg atataccggc tgatttcccc gagggggtaa ttctccagga ctttcacccc
    19321 ggtaaaggaa ggtttgaacg gcttataatc tttccaactt gctttgaacc ggttgtccct
    19381 tgcctgttcc agcgtgagat aagatattcc ggccatcgca tctttatgtt tttcccttag
    19441 ccggctgtat ttttcgttca ccgaagcgag aaaatcattc ttcattcctg ccgaaagcaa
    19501 agaagcggca acgccggcca cccttgatgc atcgcgcaca tgcaccaccg gatattgata
    19561 ctgcggggtg atcttcacgg cggtatgcat ttcgctggta gtggctccac cgatgagcag
    19621 cggtactttt aatccgcgcc tttgcatttc cgaagctaca aaggccattt catccagcga
    19681 aggagtgatt aaacccgaga gcccaacaat atccactttt tcgcgaacgg ctgtttcgat
    19741 tatcttctcg gcgttgacca tcacacccag gtctatcacc ttgtagttgt tgcagccaag
    19801 aaccacgctg acaatgtttt ttccgatgtc gtgcacatcg ccttttacgg tagccaacag
    19861 aattttcccg gcagaaatct ttcccgcgga agctctttcg ttttcgagat aagacaagag
    19921 cacggaaacg gcttttttca taacgcgggc gcttttcacc acctgcggca gaaacatctt
    19981 cccggcttca aaaagatcgc ccgccgcctt catcccattc atcaacggcc cttcaattac
    20041 tccgatagca tgagtaaaca gcaaccgggc ctcttccaca tcggtggcaa taaaagtatc
    20101 gattccgtga attatagcat acgatatcct gtcttcgaca ggcagcccgc gccatgcttc
    20161 ggcaacagat tccttcctta ccgaagttct catctgggaa gtataactga taagacgttc
    20221 cgtggcatcg ggattccggt tgaacaggac atcttcgata tgttgaagca ggccggcagg
    20281 aatgtcatca taaaccggaa ggttgccggc gttcacaatg cccatatcaa ggcccgcctt
    20341 gattgcatgg aacagaaaag ccgaatgcat ggcttcacgc agggcatcgt tgccgcgaaa
    20401 cgaaaacgag aggttgctga tgcccccgct gacccgcgca tagggaaggt tttgctttat
    20461 ccagcgcgtg gcgttgagaa aatcgagagc ataatggttg tgttcttcga tgccggtagc
    20521 gatagccagg acatttgggt cgaagataat gtcttcgggc ggaatgccga gttcatcggc
    20581 gagaatccgg taggcacgtt cgcagacagc gatgcggcgc tcataggtcg cagcctgccc
    20641 ctgctcgtcg aaggccatta ccaccatggc tgcgccgtaa ttccttattt taagggcatg
    20701 ctctttgaaa atggcttcac cttccttcag gctgatcgaa tttacgatag ctttcccctg
    20761 caggcatttc agcccggcct caatcacgct ccatttgctg gaatctatca ttaccggcac
    20821 tttggcaatt tccggctccg acatcagcaa attcagaaag cgggtcattt cgcgctctgc
    20881 atcaagcatg gcatcatcca tattcacatc cagcgcctgg gcgccggcat ccacctgctg
    20941 tcgggcaacc gaaagggctt catcatattt ttcctcacgg ataaggcggg caaatttcct
    21001 ggatcctgaa acgttggtac gttcaccgat attgatgaag ttgctcttct cataaaccat
    21061 cagcggctca agcccgctaa gcttcaggtc atgatttttg ggaggaatat gccggacttt
    21121 tgcctcttca gctatccggc ataactcacg gatatggtca ggcgtggtgc cgcaacaccc
    21181 accgataatg ttcaccgaac cattgacgag caaatcgctg atatgcttcg ccatctttgc
    21241 cggcgtttca tgataatgcc cgaactggtc gggcaggccg gcattagggt aagcgcttac
    21301 ataacaaggg gctttacgcg aaagctcctc aatgaagggg tttaactgct ccgcccccag
    21361 cgaacagttg aagcccacgc tcagcaaatc cgtatgcgaa accgaattca gaaatgcttc
    21421 tgccgtctgc cccgaaaggg tgcggccact ggcgtcggcg atggttcccg aaaccatcac
    21481 gggaagccgg atcccttttt ccaaaaacag cgtattgatg gcaaaaagcg cggctttggc
    21541 gttgagggta tcgaaaaccg tttcgatgag gagcaggtca acgccgccat caatcagtcc
    21601 acggacctgt tcggtatagg cctccgcaag ctgatcgtaa gtgatggccc ggaacccggg
    21661 atcgctcaca acaggcgaca tgctggccga tttgttcgtc ggtccgatgg aaccggcaac
    21721 aaaccgctgt ttgccgggtg ttttctccgc aaaaccatct gcttcattta cggcaagccg
    21781 ggcggcagca aagttgatct cgtaaacata cgcttcgagt ttataatcgg ccagggatat
    21841 tgatgtggcg ttaaaggtat tggtttcgat gatatcggcg ccggcttcca gataagcccg
    21901 gtgaatgccg tcgataatct gcggttgggt caggcagagc aaatcgttgc aacctttcag
    21961 gtcaacagaa tgatggctga atcgttcccc gcgataatcg ctttcggtga gtttgtggcg
    22021 ctggatcatg gttcccatcg cgccgtcaag caccaggacg cggtggctga gttccgaaaa
    22081 tatgtccttt ttaccggtcg tcatacctta tttatataga cgcttcgctt cctgcataca
    22141 aagatactga tgcagaaacg tttatgttac gcaatcaaaa acacattcaa aaatgccggt
    22201 tgacgggatc aggagtgtgt ttcaattata atctcccgcc ggggcgggat caggtattgg
    22261 caccgttcgc acggctgcga tggttgccag aggttcgcag agcctggtct ctcccctctt
    22321 ctgtataact caaacaccct ttacaggaag gattatttga agggacaaaa gtacggaaaa
    22381 tttccgcata tcaaaacggg cctgaaactg accatgttat taactggagt ttcctcaaaa
    22441 attaaggccc tagatcggat aggccaataa g
//
LOCUS       DAOPYV010000049        22471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_2341_length_22471_cov_11.173938, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000049 DAOPYV010000000
VERSION     DAOPYV010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22471
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2341_length_22471_cov_11.173938"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1361)
                     /locus_tag="PKI35_07680"
     CDS             complement(69..1361)
                     /locus_tag="PKI35_07680"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNX44296.1"
                     /translation="MLKKLISTLGIRTFSAAVNLAIAVLLSQYLGPAGKGTQSIIITT
                     ISFILIFSNFVGGATLVYLAPRYPVEKLLMPSYAWTLLTGIFSFVVLSVLSLFGNRYA
                     LVESRYIIHVCVLSILNSVTGIHSNILLGKEQVHKSNLLVLFQSLFLVLCLLLVFTVF
                     GQWTVDAYVWSLYISFGLCLVLSTLYIRSFIPKIRIFSLGEYIPVVREMFRVGIQNQV
                     AHITQLLSFRLSYYVLEKYKGPAALGVYSNGISIAESIWLVAKSMALVQYSWVSNSKD
                     RDESAKLTIKLVMVCLVLSLLILIPLLILPAGAYTWIFGEGFEGVRPVIWTLAPGVAI
                     YNLSILFGHYFSGTGRYYRNSGISTAGLVVSAVLYFTLIPAFSVTGAGIATSISYLFT
                     SLLFLWHFAREYGNWWKEMFPKRSELKAMLAQIRSKIK"
     gene            complement(1375..2565)
                     /locus_tag="PKI35_07685"
     CDS             complement(1375..2565)
                     /locus_tag="PKI35_07685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX44297.1"
                     /translation="MSQHKKILFLPRWYPNRYDPMPGLFIRRHAISVSGMFDVAVLYV
                     HRNSEKQERKFEIDRNNGFGISEWSIYFRANACKVNGIASLINSFRFFRAHIIGFRLV
                     RKEFGSPDLIHVNVLTRLGFVALFYKKLKKVPYVITEHWTRYLPGMNNFRGWWRKRIT
                     KVVVKQASAVMPVTLNLQHAMERHGLLNQHYTVIPNVVDTSLFTPDYSKKDKSAASIL
                     HVSCFDDRQKNISGILRVLKKLSGKRQDWHFLFVGEGIDFEKLVQYAGSLDFEDGLIE
                     FAGLKENKELASIMQQADFQVMFSRYENLPVVIPESFACGVPFISSDVGGIAEHIHPG
                     LGILVPSENEESLIRAIEYLLDHREEYSKEVIRQYAEDHFSREVIGRQIAAVYNSILG
                     SIQR"
     gene            2676..3701
                     /locus_tag="PKI35_07690"
     CDS             2676..3701
                     /locus_tag="PKI35_07690"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNX44298.1"
                     /translation="MTVLKSYLTLFVLALSILPLTSPAQEIAPYQVKWRSVSEVKELQ
                     QKITRPILIFLYEPDNDSSKLLLENSFNKSSVVDYVNAAFYPILFDVTTDSVIEYFDN
                     NTYRKQPGNHYNDLVLKLAGDKPALPLLVLYNRSAKGYTFSGYKSQEELVCMLTFIAE
                     NVDLTTEFNVWAKEFRHTYQPESDTAQKPSSIRWMSLKEALAANKNNPRYMFMTWYTT
                     WSTPCNVMLHNAFADEYITRFMNEHFYCVRLDAQTMDTLFWGKNYINENKPHHYHQMA
                     MSMLEGKLLFPANIFFDKDGQLIQRDQVFLDKADFYMLANFIATGSYKTMKVSDYRKT
                     FYQLDKK"
     gene            complement(3736..4278)
                     /locus_tag="PKI35_07695"
     CDS             complement(3736..4278)
                     /locus_tag="PKI35_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX44299.1"
                     /translation="MEKKTKKRPIKVAVTGPESTGKSWLAEHLAGHYGTLWVPEYSRE
                     YLQAIGRPYDYNDILKIAEGQLKAENAALEKPGDHRLLFCDTEFIVTKIWCEDKFGRC
                     HEYIDSLVRNHQYDLYLLCDIDLPWEFDPLREDQHRRPFLFDLYRETLESNRLRYGIV
                     SGSGTERLENAVRMIDKLLA"
     gene            complement(4248..4859)
                     /gene="pnuC"
                     /locus_tag="PKI35_07700"
     CDS             complement(4248..4859)
                     /gene="pnuC"
                     /locus_tag="PKI35_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNX44300.1"
                     /translation="MAISTILDSLYQGLLATTWVEFVAVIFGLLSVWFSRNENILTYP
                     TGIINVLIYIYLCFTAGLYADMGINAFYFVMSVYGWYNWSRRDMNSKHVPITKLSAWG
                     WTYNIILVFGFFGLLWFVLARYTPSTVPMFDSFTTALFIVGMWLMARKKVENWLAWIA
                     GDILVIPLFAYKGLVFTGFQYMVFTALAISGYIEWRKKLKSGQ"
     gene            4967..5533
                     /locus_tag="PKI35_07705"
     CDS             4967..5533
                     /locus_tag="PKI35_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44301.1"
                     /translation="MKFNRYPLILFVFLALVLTGCLTCEKKEYVFEVTGPESGKLTIR
                     YINIFSNSIDSTGEVKADYDELVGMWLNGEKIERDYPKAVKIRKRLYESGGQLCGEVT
                     LEFRDLNDVRLFRYDENSPYMLNTTTMADDGEVFQNSNGDFGGDNMPVIFWRPDNKTL
                     RLTTTIARPDSTTVSLLDTWKANSGKKK"
     gene            complement(5539..6900)
                     /locus_tag="PKI35_07710"
     CDS             complement(5539..6900)
                     /locus_tag="PKI35_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNX44302.1"
                     /translation="MNIHFIAIGGSAMHNLAIALYKKGYHVTGSDDEIFDPAKSRLQK
                     YGLLPEQYGWFPEKLNHEIDAIILGMHARGDNPELARARELNLKIFSYPEFLYEQSKD
                     KTRVVIGGSHGKTSITAMILHVLKHAGIDTDYMVGAQLDGFEVMVKLSDSARYMVIEG
                     DEYLTSALDRRPKFHVYKPDIALISGIAWDHMNVFPTFDNYVEQFRIFAGLVPEDGAL
                     VYCSQDETVKEVALTAAGNFRKIPYSIPPYRVENGTTILQTRQGDVRLMVFGKHNLMN
                     IEGAHGVCKLLGVEDTDFYKAISTYKGANNRLELLASNDSTSVYRDFAHAPSKLRATI
                     NAVKEQFPGRKLTACFELHTYSSLNKDFLHQYRNAMNEADEAIVFFSHHALELKKLPN
                     LSVPDIKLAFNNIKLEVYTDSVKLQDALLKHDWKNRNLLMMSSGNFEGLDVKLIAGDI
                     TAG"
     gene            7048..7884
                     /locus_tag="PKI35_07715"
     CDS             7048..7884
                     /locus_tag="PKI35_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX44303.1"
                     /translation="MMKLIPVVADVWKMDGGVSFGVVPKSIWQKICPSDENNMIDAVD
                     RCLLLDTGDRRILIDTGMGRKRNEKYYRHRYVNPEITLEISLAEQGYAPGDITDILFT
                     HLHDDHVGGATVHDNSGNAVPAFPNATFRCSAAQWEWAIHPNRREAATYHPDNLWPLY
                     DSGRLRFIESEGEFIPGVTLKIFNGHTVGQIIPFIETGNRTVVFMGDFIPTAANIPLV
                     YIPAVDIQPLVSMAEKEAFLGEAVTNNFTLFLEHDFHNECCTVSETEKGFVSGQCFRF
                     CA"
     gene            8062..8355
                     /locus_tag="PKI35_07720"
     CDS             8062..8355
                     /locus_tag="PKI35_07720"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HNX44304.1"
                     /translation="MITKRQFLKTKPVCKVTFRLIAPAEHVCIAGDFNSWSAGSTPMK
                     KGKDGSFTITIDLETGREHQFRYLLDGKTWINDEAADRYERTPLGNENSVVSL"
     gene            complement(8368..8835)
                     /locus_tag="PKI35_07725"
     CDS             complement(8368..8835)
                     /locus_tag="PKI35_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44305.1"
                     /translation="MKRFSKDGLLIIPVPEKKETESKNVIVIRECYCQNGHSLISNRI
                     KFGEFEGLLVAVEKGSAKGLIALSPVYGEKYRVSIDIGVEEGELLTLKCPECGAKLQS
                     YGPCSCGGELVVMFTKPVPDFNYCIGICNRVGCPHSEIKNEGQLMALTLYDSL"
     gene            complement(8937..10157)
                     /locus_tag="PKI35_07730"
     CDS             complement(8937..10157)
                     /locus_tag="PKI35_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX44306.1"
                     /translation="MNFELFISRRLIRGGKGSFSGPVVHIAVASVAIGLAVMIVAVAI
                     VTGFKKQIQEKITGFAAHIQIAHFDANASFELKPIDRYQPCYNKLKNTENIRHIQVFG
                     TKAGIIKAGDQMQGVVFHGVGSDFDWSFFRDKMVDGKVFRVKDTAANDSVIVSRSLAK
                     LLNLKTGDPLRMYFIIDNKARGRKFLISGIYETGLEEFDQLYVFGDLAHIQKLNGWNS
                     SQVSGFEVFIRDFRDLDKMGEVVYHKIGYDLNARTIRDIYPQLFDWLSLQDMNVIIIL
                     ALMVMVAGITMISTLLILILERTGMIGMLKALGAKNLSIRKVFLYNAGYIIGKGLFWG
                     NVVGLALGFIQYKFHVIALPQESYFMSYVPINLNLINILLLNAGTVLICMCMLIVPSF
                     IITRISPVRAIRFD"
     gene            10414..11628
                     /locus_tag="PKI35_07735"
     CDS             10414..11628
                     /locus_tag="PKI35_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNX44307.1"
                     /translation="MKFQKTAFLIFLLFFTTSWCRSQEVCNKVTASPAIRPAAESMDQ
                     YLSLLTNKNVGLVANNTSLVGKIHLVDTLLRSGIRVVKIFGPEHGFRGNASDGDRVGN
                     ATDPETSIPVISLYGDHRKPSSGDLSGLDVVVFDIQDVGCRFFTYISTMTYVMDACAE
                     KGLPMIILDRPNPNGYYVDGPVLEPKYRSFVGLHPVPIVYGMTIGEYALMVNGENWLM
                     DGMHCNLTVIPCSGYTHSSRYQLPVKPSPNLPVMESVYLYPSLCLFEGTSISVGRGTD
                     KPFTMIGHPLFHEGSFIFTPKSVPGVSDNPPFKGKTCYGQDLEAAAAGIKENGRVELG
                     WLTGMYKYMRGRSEFFTPYFEKLAGTARLREQIESGMTSKAIRRSWQKDIRQFMKIRE
                     KYLLYPDFQNHL"
     assembly_gap    11857..11956
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12174..12992)
                     /locus_tag="PKI35_07740"
     CDS             complement(12174..12992)
                     /locus_tag="PKI35_07740"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HNX44308.1"
                     /translation="MKRIIIKILVLAAIVTGFASCQKELMTPMTGENTAVVEAYLVAG
                     EPSGLVYVSRILPFSDDTVNIKEMIAGLKLYINGIPLTEIGTGIYKFELTDGSMINTD
                     STYILSFEYGGNEVTASTIIPSKPTGFTSSASTIYATRILDDSTFAPGSMMDEVEFTW
                     DNDDASYHYLMVEYLEDSADYINANMAEMDLPFNQATSPTQETYFKVGMQNIRFFGSY
                     RIVLLKVNEEFNDAFTRNGTNSNNLVNPNTNIANGYGLFTGVSSDTVYLEVVPE"
     gene            complement(13053..15794)
                     /locus_tag="PKI35_07745"
     CDS             complement(13053..15794)
                     /locus_tag="PKI35_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX44309.1"
                     /translation="MDHCRTLSLKVVVIASLSFILASGALMAQEYADTKINEYYWGTP
                     LIKVIKDLKFKYHIPIVYDSAMVSGYKLDYLFDNTNAKMALEIIFRDNKDLSYMIDSN
                     NLVQVIPRELAKGNMITGSNLKYQTKPQRFNLTVTGVIKDKLSGEPLPFASVLIRENS
                     AGTSSNVDGYFTLQHVPSDTSAFIVFYLGYYRQTFHLTPKSDLTKLVVYLDPMPTELK
                     AVEVKGEREELMRASEGGNMIKMAPAKIAELPSLGEKDIFRTFQLMPGIGGCNESSSG
                     LYVRGGTPDQNLILYDGFTVYHQEHLFGMFSAFNPNAVKEVQLYKGGFESKYGGRLSS
                     VMEIIGKTGNEEKFNAGVDAGFLGINGYMEIPLNHKGSVFVAGRRSYKTFLYEKLFDS
                     FSQTSETSKPKVTVPDMGSGPPGMRQMEEEQSPTSYFYDLNFKATYPITRKDILSYSY
                     FSGQDDYDNSRDLSRERGGISMEGDVTDLTTWGNWGTSLKWSRKWSEKTYTNNLISYS
                     NYKSSKDRSSSRTFVNEDDETETFNSGTIEDNNLKDLCFKTDNELKTGRHNQLEFGAQ
                     VNYYNIKYNFIQNDTVTVLDMNNSGSLAALYIQNRWKPLDKFMFLPGIRMSYYDNTGK
                     LYFEPRFQASYNLTEQLKIKGSTGVFYQFANRIIREDIASGSRDIWVLSNGDNIPVSK
                     AIHVIGGACYENRNYLIDAEAYYKKLTGLTEYTLRFTQQFGPDQTVDYSSLFYNGEGY
                     TRGFDVLFQKKYGKYSGWIGYTLSETRYHFPVYGNGYFSATQDVTNEVKIVNTYKYKK
                     WTFSATWIFGTGMAYTRPLGGYVLTLPDSTTVDVIIPGSKNAARLPDYHRLDVSCKYD
                     FRMGEFGKGSLSFSVFNLYNRKNVWYKEFEVDETGLTETNMTLLGITPNISLSVQIR"
     gene            16213..17883
                     /locus_tag="PKI35_07750"
     CDS             16213..17883
                     /locus_tag="PKI35_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HNX44310.1"
                     /translation="MEWIRDLLMNESVAQTVIIYSFVIAVGVALGKVKIYNISLGITF
                     VLFAGIAIGHFGFRVNHEVLDFAKDFGLILFVFAVGLQVGPGFFSSFRKGGLTLNLLA
                     VMIVLLGGVTVVILQYITGISMPVMVGIMSGAVTNTPGLGAAQDAYKEAASGMQGSEM
                     ADIGLGYAVAYPFGVLGIILTMMLIRRATGTDMAEEIVQYERLQHPEENLPKSVCITI
                     TNPSVTGKKIRELKPLLHNDAVISRVLHEGEMSVAHSGSLLCEGDVILLVAENGDIPG
                     LIQNFGKESDMDLSAQPGKLMSKQVLVTCSSVNGKSLESLNIRSRFDVNITRVYRAGI
                     ELVASPKFRLQIGDKITVVGDENAIEGVARLLGNSLRRLNEPNLFPIFIGILLGVLLG
                     SIPVAIGNMPTPVKLGLAGGPLIVAILLSKFGYKLSLTSYTTPSANLMLREIGIVLFL
                     ASVGIKAGEKFIPTLVSGDGFLWMGYGSIITLAPIILAGLFTKIVLKWNFFEVCGLLA
                     GSMTDPPALAFANSIAQSEAPAVAYATVYPLVMFLRIVIAQLLVMLFV"
     gene            complement(18038..18433)
                     /locus_tag="PKI35_07755"
     CDS             complement(18038..18433)
                     /locus_tag="PKI35_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNX44311.1"
                     /translation="MSAAVAAIIRNPQNEILFTVRRHDPAKGKLDLPGGFADPGETAE
                     EALVREIKEELNLDVKEFRYLRSFPNHYPFGGFVYQTLDMVFECAVTNPDEIIPADDV
                     CGYRFIDIRHLDPEEIGLPSIRNIVQFLR"
     gene            complement(18562..21492)
                     /locus_tag="PKI35_07760"
     CDS             complement(18562..21492)
                     /locus_tag="PKI35_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNX44312.1"
                     /translation="MNLNEKIAGLGKNFRGDIYTDDSTLVMYATDASAYRELPLAVLR
                     PKDKKDISEIIRFAKENRIPVIPRSAGTSLAGQVVGRGLVVDVSKYMTRVLEINEAEG
                     WVRVEPGVVLDELNKLLEPKSLFFGPGTSTSNRCMIGGMVGNNSCGTHSLVYGSTRDH
                     TLEIRAFLSDGTEVVFGPLTNGEFTIKYSQEGLEGDIYRNIRDILSDTRNQQEIREQY
                     PDPSIHRRNTGYAIDLLLETAPFSAGAGEFNFCRLIAGSEGTLCFVTEIKLNLVPLPP
                     KEKALMCVHCHSLEEAFYGNIIALKHKPVAVELMDNTILEQTKANITQRRNRFFLQGD
                     PEAVLIIEMAEPSKEALDAKATAVEAELRSAGYGYHYPMVYGSDISRVWALRKSGLGV
                     LSNMPGDAKPVPVIEDTAVNPEALPDYMADFRQMLRNLDLACVFYAHIGTGELHLRPV
                     LNLKDPKDVERFHTVALETAKLVKKYRGSLSGEHGDGRLRGEFIPLMIGDHNYHLLES
                     IKHTWDPDGIFNPGKITATPRMNSSLRYDAVRKAEEPETYFDFSSTHGLLRAAEQCTG
                     SADCRKSSVMGGTMCPSFMATLDEKNSTRARANILREFLTRSTKQNRFDHKEIYEVMD
                     LCLSCKGCKSECPSNVDMARYKSEFLQHWYDKHGIPFRSRMIAYITYINKLGMIFPGI
                     FNFFAGNNFTSSLLKKILGFAPQRSIPKLCKTSLRRWLKSKGNCCGEKGFPNGKVFLF
                     ADEFSNYNDVEIGIKAIKLLNRLGYRVEIPRHTESGRTYISKGLIRKVKKIARYNVGN
                     LSGLVSESVPLIGIEPSAILTFRDEYPDLVGVTLRQQALDLAPNCLMFDEFIMKEVQK
                     GNISKEAFVAEKRDILLHGHCHQKSLASTGPTKEMLSLPVNYSVTEIKSGCCGMAGAF
                     GYEKEHYEFSQKVGELVLLPEVRKAPPETIIAAPGTSCRNQIYDGTGRKACHPLEVLY
                     DALK"
     gene            complement(21501..21881)
                     /locus_tag="PKI35_07765"
     CDS             complement(21501..21881)
                     /locus_tag="PKI35_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNX44313.1"
                     /translation="MKNLKKHYRIKALPEFVYKALTNPLSIEMWSGAEAVMEEKAGTE
                     FSLWDGDITGLNLEFTENQKIVQQWYFEGQAEKSLVTITLAPEGRETRVEVLHINIPD
                     EAFDNIVDGWDNYYFKPLKKLVEE"
BASE COUNT         5745 a         5347 c         5276 g         6003 t
ORIGIN      
        1 cagaatccag ggaacatttc gtgataattc gtgtcccggc gcagcctgtt tcgtgaaatt
       61 cgtggttatt attttatttt acttctaatc tgagccagca tcgctttaag ttcactccgc
      121 ttcgggaaca tttctttcca ccaattaccg tattccctgg caaaatgcca taaaaagaga
      181 aggctggtaa acagatagga tattgatgtg gcaattccgg cccccgttac cgaaaaggcc
      241 gggatgaggg taaaatagag caccgccgat acaaccagcc ctgcggtgga aatgcctgaa
      301 ttcctgtaat atcgccctgt tcccgaaaaa taatgcccga agagaataga caggttataa
      361 atagctactc ccggggccag cgtccagatg accggtctga ctccttcgaa accttccccg
      421 aaaatccatg tataggcgcc ggccgggaga ataagcaaag ggatcagtat gagcaggctc
      481 agcacgaggc aaaccatcac cagtttgatg gttagttttg ctgattcatc acggtctttc
      541 gaattggaca cccaggaata ctgaacaagc gccatgctct tggcaaccag ccagattgac
      601 tcggcaatcg aaatgccatt gctgtaaact cccaatgcgg caggaccttt gtatttttcc
      661 agcacatagt agctgagccg gaaactgagt aattgggtga tatgggcaac ctggttttgt
      721 atcccgacgc gaaacatctc tcttaccaca gggatgtatt cgcctaagga aaatatcctg
      781 attttcggaa tgaaactccg gatgtataac gtgctgagta ccaaacaaag gccaaacgaa
      841 atatacagcg accacacata ggcatcaacg gtccattgcc cgaataccgt aaaaaccagg
      901 agcaaacaca atacgaggaa gagtgactgg aataatacca gaaggttcga tttatggacc
      961 tgttctttcc ctaagaggat gtttgaatga atacccgtaa ccgaattcag tatcgaaagc
     1021 acgcagacat gaataatata acggctctca acaagagcgt agcggtttcc gaaaaggctc
     1081 aggaccgaca atactacgaa ggaaaagatg cccgttaata aagtccaggc ataggatggc
     1141 atcagcaatt tttcaaccgg gtatcggggc gcgagataaa ccagcgtagc cccgccaacg
     1201 aaatttgaaa agatgaggat aaaactgatg gtcgtgatga tgatactctg ggtacccttg
     1261 cctgccggac caaggtattg cgataacaaa acggctattg ccaggtttac ggccgccgaa
     1321 aaagtcctga ttcctaaggt actgatgagt tttttcagca tgatgcggcc ggtttcagcg
     1381 ttgaatactt cccagtatgc tgttataaac cgcagcaatc tgccggccaa tcacttccct
     1441 gctgaaatgg tcttctgcat actgcctgat gacttctttg ctgtattctt cacgatgatc
     1501 gagcaagtat tcaatagccc gaatcagcga ttcttcattt tcggaaggca caaggatacc
     1561 gaggcccgga tgaatatgtt cggcaatacc gccaacgtct gaagagatga acggcacccc
     1621 gcaggcgaaa ctctcgggaa taaccaccgg gaggttttca tatctgctga acattacctg
     1681 aaagtctgcc tgttgcataa tcgacgccaa ctccttgttt tcttttaaac ctgcaaattc
     1741 aattaatcca tcctcaaaat ccaggctgcc ggcatattga accagttttt caaaatctat
     1801 accttctcct acaaacagga aatgccagtc ctgtctcttt ccggacagct tttttaaaac
     1861 gcgcaatatc cctgaaatat ttttctgcct gtcgtcgaaa caggaaacat gaagaattga
     1921 agctgccgat ttatcctttt tcgaataatc aggcgtaaag agggatgtat ctactacatt
     1981 gggaataacc gtgtagtgtt ggttcagcaa tccatgtcgt tccatggcat gttgaaggtt
     2041 cagggtaacc ggcatcacgg ctgatgcttg tttcacaacc actttcgtta tcctctttcg
     2101 ccaccatccc ctgaaattat tcattccggg gagataacgc gtccaatgct cggtaattac
     2161 gtaaggtacc tttttcagct ttttgtaaaa cagcgcaaca aatcccagcc gggtaagaac
     2221 attgacatgg atcaggtcag gactcccgaa ttcctttctt accaggcgaa acccgataat
     2281 atgggccctg aaaaaacgga aagagtttat aagcgaagca ataccgttta ccttacaggc
     2341 atttgcacgg aaatagatac tccattcgga aatgccaaac ccgttgttcc tgtcaatttc
     2401 gaattttctt tcctgttttt cactgttcct gtgaacatat aatacggcga catcaaacat
     2461 tccggatacc gatatggcgt gtctcctgat gaacagtccc ggcatcggat cgtaccggtt
     2521 ggggtaccaa cggggtaaaa aaagtatctt tttatgctga ctcattatgc tgcttaggat
     2581 ggtcaaaagt aaaaaatctt cgtaaaaggc agcgattata tccggccttc acaaataata
     2641 acgaatttta ccccctgaaa tcattgaccc acattatgac cgtgcttaaa tcctacctga
     2701 ccctctttgt tcttgccttg tctatattac cgcttacctc accggcacag gaaattgccc
     2761 cgtaccaggt aaaatggcgc tctgtttcag aagtgaaaga acttcagcag aaaatcacac
     2821 gaccgatcct gatctttctt tatgagccgg ataatgattc gtcgaaactc ctgcttgaaa
     2881 atagtttcaa caagagttcg gtggtcgatt atgttaatgc tgcattttat ccgatacttt
     2941 tcgacgttac taccgattca gtcattgaat atttcgacaa taacacatac agaaaacagc
     3001 ccggcaacca ttacaacgac cttgtcctga agttggcagg ggacaagccc gccctgccct
     3061 tgcttgtatt atacaaccgc agtgcaaaag gatatacgtt ctcagggtat aagagccagg
     3121 aggaactggt atgcatgctg acctttatag ctgaaaatgt agacctgaca accgagttca
     3181 atgtctgggc caaagagttc cgtcatacct atcagcctga aagcgacacg gcgcagaagc
     3241 cgtcatcaat ccgctggatg tcgctgaagg aagcgctggc agcaaacaag aataacccga
     3301 ggtatatgtt tatgacctgg tatacaacct ggagcacgcc ctgcaatgta atgctgcata
     3361 acgccttcgc cgatgaatac atcaccaggt tcatgaacga gcacttctac tgcgtccgcc
     3421 ttgatgccca gaccatggat acattattct ggggcaagaa ttacataaat gagaacaaac
     3481 cccatcatta tcaccagatg gcgatgtcga tgcttgaagg aaagctgctt ttccctgcaa
     3541 atattttttt cgataaagac gggcagctta tccagcgcga tcaggttttt cttgataaag
     3601 ccgatttcta tatgctggcc aattttatcg ccacaggctc gtataaaaca atgaaagtaa
     3661 gcgattatcg taaaactttc tatcaactcg acaagaaata accgggctat gccatgatgg
     3721 cgccggaacg atgattcagg ccagcagctt atcaatcatt cttacggcat tttccaaacg
     3781 ctcagttccc gagccgctga ctatgccata acggagccgg ttgctttcca gcgtttctct
     3841 gtaaagatca aaaaggaatg gccgccggtg ctgatcttca cggaggggat cgaattccca
     3901 gggcaggtca atatcgcaca acagatacaa atcgtactga tggttcctga ccagtgaatc
     3961 aatatattcg tggcaacgcc cgaatttgtc ttcgcaccag attttcgtca cgatgaactc
     4021 tgtgtcgcaa aagaggagcc gatgatctcc gggtttctcc aacgcggcgt tctcagcttt
     4081 caactggcct tcagcaattt tcaggatgtc gttatagtca taaggccgtc cgattgcctg
     4141 taaatactcc cgcgaatact ccggcaccca aagggttccg taatgacccg ccaggtgttc
     4201 ggccagccag gatttgccgg tcgattcagg tccggtaacg gccactttta ttggccgctt
     4261 tttagttttt ttctccattc tatgtatccc gaaatggcca aagccgtgaa aaccatgtac
     4321 tgaaagccgg taaataccag gcctttgtat gcaaacaacg gtattaccag aatatcgccc
     4381 gctatccatg ccagccagtt ttcgaccttt ttcctggcca tgagccacat acccacaata
     4441 aacagtgctg ttgtaaacga atcgaacatt ggcaccgtac ttggggtgta tctggccagc
     4501 acaaaccaca gcaacccgaa gaaaccaaat acgagaatga tattatatgt ccacccccag
     4561 gccgacagtt ttgtaatggg aacatgcttg ctgttcatgt cgcggcggct ccagttatac
     4621 catccgtaaa cgctcatcac aaaatagaat gcattgatcc ccatatcggc ataaagtcct
     4681 gccgtaaaac agagatagat ataaatcagc acattgatga tgcccgttgg gtaagtcagg
     4741 atgttttcat ttcgcgaaaa ccaaacgctg agcagtccga aaataacggc aacaaattcc
     4801 acccatgtgg tagccagcag tccctggtat agcgaatcga gaatggttga aatagccaaa
     4861 attcaggtgc gttttaatcg gggcaaaggt acaattcgca ttgaacaata ggaaaccggc
     4921 cgaatatccg aaaatcgtac ttttgaatcc tgttaatgga tctattatga aatttaaccg
     4981 atatccgctg attctttttg tttttctggc gcttgtgctt accggatgcc ttacctgcga
     5041 aaagaaagaa tatgttttcg aggttaccgg acctgaatcc gggaaactga caatccggta
     5101 catcaacatc ttttcaaaca gcatagattc gacaggcgaa gtaaaagccg attacgacga
     5161 actggtcggc atgtggctga atggtgaaaa aattgaacgg gattacccca aagcggttaa
     5221 aatcaggaaa aggctttatg aatcaggcgg gcaactctgc ggggaggtaa ccttggaatt
     5281 cagggatctg aacgatgtac ggttatttcg ttatgatgaa aactctccgt atatgctgaa
     5341 tacaacgacc atggccgatg acggggaagt attccagaat tcaaacggcg atttcggggg
     5401 cgataacatg cctgtcattt tctggaggcc cgataataaa acccttcgcc tcaccacaac
     5461 aatcgccagg cccgacagca caaccgtgag ccttcttgac acgtggaaag ccaattccgg
     5521 aaaaaagaaa tagaagcttt accctgctgt tatatcaccg gcaatcagtt ttacatccag
     5581 gccttcgaag tttccggaac tcatcatcag caaattcctg tttttccagt cgtgcttcag
     5641 cagggcatcc tgaagtttta ccgaatcggt ataaacctca agttttatat tattaaaggc
     5701 cagtttaata tcaggaacgg aaagattggg taactttttc aattcaagcg catgatggct
     5761 gaagaaaacg atggcctcat cggcctcgtt cattgcattg cggtactgat gaagaaagtc
     5821 cttgttcagg ctgctataag tgtgcaactc gaaacaggct gtaagttttc gtccgggaaa
     5881 ttgttctttc acagcattta tggtcgcccg cagtttcgac ggagcatggg caaagtcgcg
     5941 gtaaaccgaa gtgctatcgt tagaggccag caattcaaga cggttgttgg cgcctttata
     6001 ggttgaaatg gctttgtaga aatccgtatc ttccacgcca agcagcttgc agacaccatg
     6061 cgccccttcg atattcatca ggttgtgttt accaaaaacc atcagcctga cgtctccctg
     6121 ccgggtctga aggatggttg taccattttc cacacggtac gggggaatgc tgtacggaat
     6181 cttcctgaaa ttgccggccg ctgtaagggc aacctctttc acagtttcat cctgcgaaca
     6241 ataaaccaac gcgccgtctt cggggacgag ccctgcaaaa atcctgaact gttccacata
     6301 gttatcgaaa gtcgggaaca cattcatgtg atcccaggcg ataccgctga tcagagcgat
     6361 atccggctta tagacatgga attttggcct gcggtccaaa gctgatgtaa gatattcgtc
     6421 gccctcaatc accatataac gggctgaatc gctcagtttc accatcactt cgaaaccgtc
     6481 gagttgtgcg ccgaccatgt aatcggtgtc aatcccggca tgtttcagca catgcagtat
     6541 catggccgta atggaagttt tgccgtggct tccgccgatc accacccgtg ttttatcctt
     6601 gctttgttcg taaaggaatt cagggtatga gaatattttc aggttcagtt cccgcgcccg
     6661 ggcaagctcg ggattgtcgc cgcgggcatg catccccagg ataatggcat ctatttcatg
     6721 attcagtttt tccgggaacc aaccgtattg ttcgggtaac aggccgtact tctgtaatcg
     6781 tgatttagca ggatcgaaga tttcgtcatc cgaaccggtg acatggtaac cctttttata
     6841 cagcgcgatg gctaggttgt gcatggcgct tccgcctatg gcaatgaagt gaatgttcat
     6901 agtttgatat atgctgctgc aaatttaaat ctttaagatt tcgctgataa gtggtaatat
     6961 taattgttac cggaaaatgc tgctttctgc cccgattccg aattcactcc gattgattaa
     7021 atttgcctgc caaacagtta ttatatcatg atgaaactga ttccggtagt tgcagatgtg
     7081 tggaagatgg acggaggcgt ttctttcggc gtggtcccga agagcatctg gcaaaagatt
     7141 tgtccgtcgg atgagaacaa tatgatcgac gccgtggaca ggtgcctgct ccttgatacc
     7201 ggcgatcgtc gtatactcat tgatacgggt atgggccgca agcggaatga aaaatactac
     7261 cgtcaccggt acgttaatcc ggaaatcacg ctcgaaatct ctttagcgga gcagggatat
     7321 gctccaggtg atattaccga tattctgttc actcatcttc acgatgacca tgtaggcggg
     7381 gccacggtgc atgacaattc gggaaatgcc gtgccggctt ttccgaatgc gaccttccgg
     7441 tgttcggctg cgcaatggga atgggctatt catcccaaca ggcgggaagc cgcaacttat
     7501 catcccgata atctttggcc tctatacgac agcggcaggc tcagatttat tgaatcggaa
     7561 ggcgagttca ttcccggagt gaccctgaag attttcaacg gacatactgt ggggcagatc
     7621 atccctttta tagaaaccgg aaacaggaca gtggtgttta tgggagattt tattccaacc
     7681 gccgcaaaca ttccccttgt ttacattcct gccgtcgata tccagccatt ggtaagcatg
     7741 gcagagaaag aggcttttct cggtgaagcg gtcacaaata atttcaccct gttcctcgaa
     7801 cacgatttcc ataacgagtg ttgcacggtt agtgaaacag aaaaaggttt tgtttcaggg
     7861 caatgctttc gcttttgcgc atgaaaagaa atacagaaga tatttaacgg taatcatcta
     7921 atgcttttat tggggaatgc ggccggtatc caacggcagc cgtatgaata accttgctat
     7981 ttagcagaat ttttccaaac gggatatgga tggtgtatct ttgtattgtt aaacatcttt
     8041 atattaacca attgcatcgt tatgatcaca aaacgtcaat tcttaaaaac aaaacctgtt
     8101 tgtaaggtaa ccttccggct tatcgctcct gcggaacacg tttgtattgc cggcgatttc
     8161 aacagttgga gcgccggttc cacgcccatg aaaaagggga aagatggttc cttcaccatt
     8221 accattgacc ttgaaacagg cagggaacat caattccgct accttctgga tggtaaaaca
     8281 tggataaacg acgaagctgc cgacaggtat gaaagaaccc cattgggtaa tgaaaactcg
     8341 gtagtttctc tttgagaacg aatttattta caacgaatca tataaagtga gggccatgag
     8401 ttgtccctca ttttttattt ccgaatgggg acatccgacc cggttgcaga taccgatgca
     8461 gtaattgaaa tcaggaaccg gcttagtgaa catcaccacc agctctcccc cgcaggaaca
     8521 cggtccgtac gactggagtt tagctccgca ttcaggacat ttgagcgtca gcagttcgcc
     8581 ttcttcaacg ccgatatcga tcgagaccct gtacttttcc ccatataccg ggctcagggc
     8641 tatcaatcct ttggcgcttc ctttttcaac agcaaccagc aacccttcaa attcgccgaa
     8701 ttttatcctg ttgcttatca gcgaatgccc gttctggcaa taacattccc tgatcacaat
     8761 cacgttcttg ctttcggtct cctttttttc gggaaccgga ataattaata aaccatcttt
     8821 actgaaacgt ttcatggcac tgttttttgt tgagtatgac gataaacctg aacaaaccga
     8881 tgttttgttc cattatcaaa gttaaggaat ttccatgacg gaagcaaccg gaaaatctaa
     8941 tcgaaacgga tagccctgac aggcgaaatc cgggtaataa taaatgacgg tacaattagc
     9001 atgcacatgc agataaggac agtacctgca ttgagaagta aaatattgat aaggttaaga
     9061 ttgatcggta cgtatgacat aaaataggat tcctgcggca gggctattac gtgaaattta
     9121 tactgaatga aaccaagggc taaaccaacg acattacccc agaataatcc tttaccgata
     9181 atgtagcctg cattataaag aaaaactttc cggatgctca ggtttttcgc gccaagcgct
     9241 ttcagcatgc cgatcattcc ggtcctttcg agtatgagga tcagcagtgt tgagatcatc
     9301 gtgatccccg cgaccattac catcagtgcc aggataatga ttacattcat atcctgaagc
     9361 gacagccagt cgaaaagttg cggataaata tcccgaattg tgcgggcatt caggtcgtaa
     9421 cctattttat gatacaccac ttctcccatt ttatcaagat cgcggaagtc cctgataaaa
     9481 acctcaaatc ccgacacctg ggaggaattc cagccattca gtttctggat atgagccagg
     9541 tcgccaaaaa cataaagctg gtcgaattct tccagacctg tttcgtaaat tcctgatatc
     9601 aagaattttc ggcctcgcgc tttgttatcg atgatgaaat acatgcgcaa cgggtcccca
     9661 gtcttcagat tcagcaattt cgcaaggctt ctcgaaacaa tcaccgagtc attggctgcc
     9721 gtgtccttca ccctgaaaac tttcccatct accattttat cccggaaaaa tgaccagtcg
     9781 aaatcgctgc cgacgccatg gaataccacg ccctgcatct ggtcgccggc tttgataatg
     9841 cctgccttag tgccaaaaac ctgaatatga cggatattct cagtgttttt caacttgttg
     9901 taacagggct ggtaacggtc gatcggcttt agttcaaacg aagcattggc gtcaaaatgc
     9961 gcgatctgta tatgtgcagc aaatcctgtg attttttcct gaatttgttt tttaaatccc
    10021 gttacgatag caacggcaac aatcatcaca gccagtccga tggccacgct ggcaacggca
    10081 atgtgtacca ccgggcctga gaaacttcct ttcccgcccc ggatcagacg tctggatata
    10141 aataattcga aattcattcg taaatcaatg atacaaacct acgtgaaatt cccgagctga
    10201 ccagcactgt atttttgttt ttcatggtcc ggatttccgg taaatggcag atcggcaaac
    10261 cggaaatggt tcaacgggca actggtttat aaagacgcag gaaggttcct ctttccggat
    10321 aaatatttcc tctttgccaa atccatgttc ccaactgata attgcgtaag tttggcatac
    10381 tgttttcaac tcccgtgcaa gattcgacga aatatgaaat ttcagaaaac cgcttttctt
    10441 attttcttac tctttttcac aacgtcctgg tgccgttcgc aggaagtgtg taacaaagta
    10501 acagcttcac cagccataag gcccgccgct gaaagcatgg accaatacct ttctctgctg
    10561 acaaataaaa atgtcggtct ggtggccaac aacacttccc tcgtcggaaa aatacacctt
    10621 gttgatactt tgctgagatc aggaatccgg gttgtaaaga ttttcgggcc tgaacacggt
    10681 ttcaggggga atgcttctga cggtgacagg gtcggcaacg caacagatcc tgaaaccagc
    10741 atccctgtca tttcactcta cggagatcac cggaaacctt cgtcaggcga cctgagcggg
    10801 cttgatgttg tagtattcga tatccaggat gtcggatgcc ggtttttcac ttatatctca
    10861 accatgacat atgtaatgga tgcctgtgcc gaaaagggat tgccgatgat cattctcgac
    10921 aggccaaatc cgaatggata ctatgtcgac ggaccggtac tagagccaaa gtacagatcc
    10981 tttgtcgggt tacatccggt tcctatagtc tacggtatga ccatcggcga atatgcactc
    11041 atggtcaatg gagaaaattg gctgatggac ggaatgcatt gcaatctcac agtgatcccg
    11101 tgttcggggt atacccattc atcaaggtac caactcccgg tgaaaccctc gccgaacctg
    11161 ccggtgatgg aatcggtgta cctttatcca tcgctctgcc tgttcgaagg cacttcaata
    11221 agtgtgggac gcggcaccga taagcctttt accatgatcg ggcacccctt gtttcatgaa
    11281 ggatcattca tattcacgcc gaagtcagtt cccggagtga gcgacaatcc gccattcaag
    11341 gggaaaacct gttatggaca agaccttgaa gccgcagccg ccggtataaa ggagaacggc
    11401 cgcgtggaat tgggatggct taccggtatg tataaatata tgcgcgggcg gagtgaattt
    11461 ttcaccccgt attttgaaaa attagcaggt actgccagat tacgggaaca gatagaaagc
    11521 gggatgacgt ccaaagctat acgccgatcg tggcaaaaag acatccggca attcatgaaa
    11581 atccgtgaaa aatatttatt ataccctgat ttccagaatc atttataggt ttggtgttaa
    11641 ttagtgttcc ggcgcagccg gcttcgtgta atcagtggtc aggtttttca ccacgaattg
    11701 acacgaaatg atcacgaatt gacacgaatg gattttaaag cataccggag aagcacttcg
    11761 agagaattcg tgttacaggc gcagccggct tcgtgtaatc cgtggtcagg tttttcacca
    11821 cgaattgaca cgaaatgatc acgaattgac acgaatnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnggat tttaaagcat accggagaag ctcttcgaga gaattcgtgt
    12001 tccggcgaag ccggcttcgt gtaatccgtg gtcaggtttt tcaccacgaa ttagtacgaa
    12061 tgaaccacga atgtgcgcga ataatattcc acggaaagca cggaaaagac acagaaaccg
    12121 cataagatta ttttgtgata atttatatgt aataatccga tctctttatt ttatcattca
    12181 ggtacaacct ccaggtaaac cgtatcggaa cttacaccgg taaaaagtcc ataaccattg
    12241 gcgatgttgg tgtttgggtt gacaagatta ttggagttgg tcccgtttct tgtaaaggcg
    12301 tcgttgaatt cttcatttac ctttaacagc actatccggt aacttccgaa gaaccggata
    12361 ttctgcatcc cgaccttaaa ataagtttcc tgggtaggag aggtggcctg gttaaacggg
    12421 aggtccattt cggccatgtt ggcattgata taatccgccg aatcctcaag gtattcgacc
    12481 atgaggtagt gataggaggc gtcatcgtta tcccaggtaa attccacctc atccatcata
    12541 cttcccggtg caaatgtgga atcatccagg atgcgcgtag cgtaaatggt tgatgcagaa
    12601 ctcgtaaaac cggttggctt ggatggtatg atcgtgcttg cggttacttc attccctccg
    12661 tattcaaagg agaggatata ggtactgtcg gtattgatca tagatccgtc cgtaagttca
    12721 aacttataaa ttccggtgcc gatctcagta agcggaatac cgttgatata aagtttaagc
    12781 cctgcaatca tctcttttat attgacagta tcgtcagaaa aaggcagtat ccgggaaaca
    12841 tacaccagtc cggacggttc gcccgcaacc aggtatgctt ccactacagc ggtgttttcg
    12901 cctgtcatcg gtgtcatcag ttctttctga caggatgcaa atcctgttac gatggcggca
    12961 agtactaata ttttgatgat tattcgtttc atattcggat aggtcattta gctttactgt
    13021 agtccgttaa ttatcggatg caataaggtt tcttacctta tctggacaga taaggagata
    13081 ttgggggtta ttcccagcag cgtcatattg gtttcggtga ggccggtttc gtcaacttca
    13141 aattccttgt accagacatt tttacgatta tacaggttaa agactgaaaa actcaggctt
    13201 ccctttccga attcgcccat cctgaaatcg tatttgcagg atacatccag acggtggtaa
    13261 tcgggaagac gggcggcatt cttgcttccg gggatgataa catccactgt ggtgctgtcg
    13321 ggcagggtga gaacatatcc gcccagcgga cgcgtgtagg ccattcccgt tccgaaaatc
    13381 caggtagccg agaaggtcca tttcttatac ttgtatgtat taacgatctt cacttcgttg
    13441 gttacatcct gggtcgcgct gaaatatcca ttgccgtata ccgggaaatg gtacctcgtt
    13501 tccgataagg tatagccgat ccagcctgaa tattttccgt attttttctg aaacagcaca
    13561 tcaaagccgc gggtatagcc ttcaccgttg taaaagagcg atgagtaatc aacggtctga
    13621 tcaggaccga actgttgcgt aaatcgtaag gtgtactcgg tgaggccggt gagtttttta
    13681 taataagctt cagcatcgat cagatagttc ctgttttcgt aacaggcgcc cccgataaca
    13741 tggatggcct tgcttacggg aatattatcg ccgtttgata gcacccagat atcgcggctc
    13801 cctgaagcaa tgtcttcacg gatgattcgg tttgcaaact ggtaaaaaac gccggttgac
    13861 cctttgattt tcaactgttc cgtcagattg tacgaagcct ggaaacgcgg ttcgaaatac
    13921 agtttaccgg tgttgtcata atacgacatg cggattcccg gcaggaacat gaatttgtcg
    13981 agcggtttcc acctgttctg gatgtaaaga gcagccagcg aaccgctgtt gttcatgtca
    14041 agaaccgtga ccgtatcatt ttgaataaaa ttgtatttaa tattataata gtttacctgg
    14101 gcgccaaatt cgagctggtt atgacgcccc gtttttaatt cgttatccgt tttaaaacat
    14161 aaatctttca ggttgttgtc ttcaatagtt cccgaattga acgtttcggt ttcatcatcc
    14221 tcattgacaa atgtccgcga agaggagcga tccttcgaac ttttatagtt ggaataggaa
    14281 atcaggttgt tcgtgtaggt tttttcgctc cacttgcgcg accatttgag acttgttccc
    14341 cagtttcccc aagtagtgag gtccgtgacg tcgccttcca tgcttattcc gccacgttca
    14401 cggctcaggt cgcgggaatt gtcataatca tcctggccgc tgaaataact gtatgaaaga
    14461 atatctttcc tggtgatagg gtaggtggcc ttgaagttca gatcataaaa ataagaagtg
    14521 ggggattgtt cttcttccat ctgcctcatt cccggaggcc cgctgcccat gtcgggaacc
    14581 gtgactttag gcttcgacgt ttcggatgtc tgtgagaatg aatcgaaaag tttttcgtac
    14641 aggaacgttt tataggaccg gcgtccagcc acaaataccg aacctttatg attgagcgga
    14701 atttccatgt acccgttgat accaagaaat ccggcgtcaa caccggcgtt gaacttttct
    14761 tcgttaccgg ttttcccgat tatttccatc acgctcgaca gccttcctcc atatttcgat
    14821 tcaaatcctc ctttataaag ctggacttct ttcactgcgt tcgggttaaa ggcgctgaac
    14881 atcccgaaca ggtgttcctg atgatatacc gtgaaaccat catacaggat gaggttctgg
    14941 tcgggcgtgc cgccacgcac atatagtcct gaagaggatt cgttgcaacc tccgatcccg
    15001 ggcatcaact gaaaagtcct gaaaatatcc ttctcgccca aactcggtaa ttcggcaatt
    15061 ttggcaggcg ccatcttgat catgtttccg ccttcggagg ccctcatgag ttcttcccgc
    15121 tctcctttta cctctacagc ctttagctcc gtaggcattg gatcgaggta aacaaccagt
    15181 ttagtcaagt ccgacttcgg tgtgagatga aacgtctgcc ggtagtatcc cagatagaac
    15241 acgatgaatg ctgaagtatc ggaaggaaca tgttgcaagg tgaagtagcc gtcaacattg
    15301 gaagatgttc ccgcgctgtt ttccctgatc agcaccgaag caaaaggcaa tggctcgccg
    15361 ctcagcttat ctttaataac acctgtcaca gtcaggttga agcgctgagg ctttgtctgg
    15421 tacttcaggt tcgacccggt gatcatgttg cctttggcca gttcgcgtgg aattacctgc
    15481 acaaggttat tcgaatcgat catataggaa aggtccttgt tgtcgcgaaa gatgatttcc
    15541 agcgccatct ttgcattggt gttgtcaaaa aggtagtcca gcttatatcc cgaaaccatc
    15601 gccgaatcat aaacgatcgg tatatggtac ttgaacttca ggtctttgat tactttgata
    15661 agcggcgtac cccaatagta ttcattgatt ttggtatcgg cgtattcctg agccatcaaa
    15721 gcaccggagg ccagtataaa cgaaagggaa gcaatgacaa caactttcag cgatagtgta
    15781 cggcaatgat ccatgtagaa aatttctgag cgtatatgtg gcgtttgatt aaaaaatcgg
    15841 acgaagacaa taatcagttt aatttttaat tgtgacaggt ttcccccttt acttcacttc
    15901 acgggatatt gtatgtcaac caattccgaa gtttgtttcg tgaagtatat taaattgatt
    15961 ctgtcctcgt ccgataccct aaacctaaac tgctgcaaaa gtgaatacga tgatcctgcg
    16021 attcaacttt attagacgtt taccgggaat ttaccctcgg aattgctgat ttaaccgatt
    16081 tgatgtttca ggttgcaagc gtcaaaccgg ctggcatttg atggcaaaag ccctgatgct
    16141 tacctgaaaa ccgcggcaat tagatatttt gagctgaaag aggtaagttt gcaccgcgaa
    16201 atattacccg gtatggaatg gatcagggat ctgttgatga atgaatcagt tgcccagaca
    16261 gttatcatct acagttttgt tattgctgta ggagtcgcgc taggcaaggt gaaaatttac
    16321 aatatctcgc tgggaataac ctttgtactg tttgcaggta ttgccatcgg gcatttcgga
    16381 ttcagggtca accatgaggt gcttgatttt gccaaggatt tcggactgat attgttcgta
    16441 tttgccgttg gtttacaggt tggaccgggt tttttctcct cgttcaggaa aggaggcctc
    16501 acgctcaacc tcctggcagt tatgattgta ttgctcggag gagtaacggt ggtgatcctg
    16561 caatatatta cagggatatc tatgcctgtg atggtcggga tcatgtcagg cgccgttacc
    16621 aatacgcccg gtcttggcgc ggcgcaggat gcctataaag aagcagcttc gggcatgcag
    16681 ggatccgaaa tggcagatat cggcctgggt tatgccgtcg cctatccgtt tggcgtgctg
    16741 ggaatcatcc ttaccatgat gctcatcagg cgtgccacgg ggacagatat ggcggaagaa
    16801 atcgttcaat atgaaaggtt acagcatccg gaagaaaacc ttcccaagag cgtttgtatc
    16861 accatcacaa atccttcggt caccgggaaa aagatcaggg aactgaagcc gctccttcac
    16921 aacgatgccg tcatctccag ggtattgcac gaaggagaga tgtctgtcgc ccattctggt
    16981 tcattgcttt gcgaagggga cgtaatatta ctggtggctg agaatgggga tattcccggg
    17041 ctgattcaga actttgggaa ggaaagcgac atggatttgt cggcccagcc cggtaaactg
    17101 atgtcgaaac aggtgttggt gacctgttca tccgttaacg gcaaatcgct ggaatcactg
    17161 aatatacgtt cgaggtttga tgtaaatatt acccgtgttt acagggccgg tatcgaactg
    17221 gtggcgtcgc cgaagttcag gcttcagata ggcgataaaa ttaccgtcgt gggggatgaa
    17281 aatgcaattg aaggggttgc ccggcttttg ggtaattcac tccgccgcct gaacgaacct
    17341 aacctgttcc cgatttttat cggtatcctg ctgggcgttc tgttggggag catccctgtt
    17401 gctattggaa acatgcccac acccgtaaaa ctgggccttg ccggcggacc gctgattgtt
    17461 gctatcctgt tgagtaaatt cggttataag ttgtccctca cgtcctatac gaccccaagc
    17521 gccaatctga tgcttcgaga aatcggaatt gtcctgtttc tggcaagcgt tggcataaaa
    17581 gcaggggaga agttcattcc tacgctggtt tcgggtgacg gctttctatg gatgggctac
    17641 gggtctataa ttaccctggc gccgattatc ctggctggtt tgttcaccaa aattgtcttg
    17701 aaatggaatt ttttcgaagt atgcggatta ctggccggaa gtatgaccga ccctccggcc
    17761 ctggcttttg ccaacagcat cgcacagagc gaagcgccgg cagttgctta tgccaccgtt
    17821 tatccgctgg tgatgttcct tcggatcgtg attgcccagt tactggtgat gttgtttgtc
    17881 tgaaaatcag tttccaggtt caggcttatc caaacatccg gttctcactg caggatgaat
    17941 aagaaaccgg gagaaaacag cgaggcatgc cagcggtatg ttgcccaggc tggaaatcat
    18001 gagcctgaaa attcaggaag gaagatgaaa gacaatttta cctgagaaat tgcacgatgt
    18061 tcctgatgga aggaaggccg atttcttcag gatcgagatg acgaatgtcg atgaaccggt
    18121 aaccgcaaac atcgtcggcg ggaattattt cgtccgggtt tgtaaccgcg cattcgaaaa
    18181 ccatatcaag ggtctggtaa acaaagcctc cgaaagggta atggttgggg aatgatctca
    18241 ggtatctgaa ttcctttaca tccaggttca gctcctcttt tatttccctc accagggctt
    18301 cttcagccgt ttcacccggg tcggcaaaac cgcccggaag gtcaagcttg cctttggcgg
    18361 gatcgtggcg tcggacggta aacaatatct cgttctgagg gttgcggatg atggctgcca
    18421 cggctgctga catattgagg aaataccgga atccgcaagc tttacagcaa aatgatttgt
    18481 ccgaatcctg taaaaatttc tctgagccgc aacgcgggca gaaatggaaa ggtgtttttt
    18541 ccgggttttc catcaggcgg attattttaa ggcgtcgtat aacacttcca gcggatgaca
    18601 ggcttttcgt ccggtaccgt cataaatctg gttacggcaa ctggtgccgg gagcggcaat
    18661 gatggtttcg ggcggcgctt ttcttacttc gggcaacaaa acaagttcac cgaccttctg
    18721 cgagaattca taatgctctt tctcgtaacc gaaagcgccg gccatgccgc aacaccccga
    18781 tttgatttcc gtcactgaat agtttaccgg caacgaaagc atttctttgg tcgggccggt
    18841 gctggcaagc gatttctggt ggcagtgtcc gtgcagcagg atatcgcgtt tttcggcgac
    18901 aaatgcttct ttcgaaatgt tccctttctg cacttccttc atgataaatt catcgaacat
    18961 caggcaattg ggggcaaggt ccagcgcctg ttgtctgagg gttacaccga ccaggtcggg
    19021 gtattcatcg cggaaggtca gaatggcgga aggctcgatg ccgatgagcg gcactgattc
    19081 gctgacaagg cccgaaaggt tcccgacatt gtaccgggct attttcttca ctttcctgat
    19141 caggcctttc gaaatgtaag tgcggccgct ttcggtatgc cgcgggattt cgacacggta
    19201 acccagacgg ttgagaagtt ttatcgcctt tatcccgatt tcaacgtcat tgtaattgct
    19261 gaattcatcg gcaaacagga atactttccc attcggaaag cctttttcac cacagcagtt
    19321 gcctttactt ttcagccagc gcctcagcga ggtcttgcag agtttcggga tactgcgctg
    19381 cggtgcaaaa ccgaggattt ttttcagcaa cgacgaggta aaattattgc ctgcaaaaaa
    19441 gttgaaaatg cccgggaaga tcattcccag tttgttaatg tatgtgatat aggcgatcat
    19501 ccgcgagcgg aacgggatgc catgtttgtc gtaccagtgt tgcaggaatt ccgatttgta
    19561 gcgcgccata tccacgttgg acgggcattc gcttttgcat cccttgcacg acaggcacaa
    19621 atccatcacc tcgtaaatct ccttgtggtc gaagcggttt tgttttgttg aacgggtcag
    19681 aaattcacga aggatgttgg cccgggcccg ggttgagttc ttctcgtcga gtgtggccat
    19741 gaaactgggg cacatggtgc cgcccatgac ggatgatttg cggcaatcgg ccgaaccggt
    19801 gcattgttcc gctgccctca gcagcccgtg ggtcgatgaa aaatcgaaat aggtttcggg
    19861 ctcttccgct ttccgaacgg catcatagcg caggctgctg ttcatccgcg gtgtcgccgt
    19921 gatcttgccg ggattgaaga ttccgtcggg gtcccaggta tgtttgatgc tttccagcaa
    19981 atggtagtta tgatcgccga tcatcagggg gataaattcc ccgcgcagcc ggccgtcgcc
    20041 atgttccccg ctgagcgacc cgcggtattt tttgaccagt tttgccgttt cgagcgctac
    20101 cgtatgaaag cgttccacat ctttcgggtc tttcaggttc agcacagggc gaaggtgcag
    20161 ttcacccgtg ccgatatggg cataaaacac gcaagccaga tccagatttc ttagcatctg
    20221 ccggaagtcg gccatataat cgggaagcgc ttccggatta acggccgtgt cttcgatcac
    20281 gggaaccggt ttggcatcgc cgggcatatt ggagagcacg ccgagccccg acttgcggag
    20341 cgcccaaacc ctgctgatat cgctcccgta aaccatcggg taatgatacc cgtaaccggc
    20401 agatcgcagt tccgcttcca ccgccgtggc tttggcgtca agggcttcct tcgaaggttc
    20461 cgccatctca atgataagga cggcctccgg gtctccctgc aggaaaaacc ggttacggcg
    20521 ttgcgtgatg ttggccttgg tctgctccag gatggtgtta tccatcagct cgacggcgac
    20581 aggcttgtgt ttcagcgcga tgatattgcc gtaaaatgcc tcttcgagcg aatggcagtg
    20641 gacgcacata agcgcctttt ccttcggcgg gagcgggacc aggttcagtt tgatttcggt
    20701 gacgaaacac aaggtccctt cggaacctgc tatgagtctg caaaaattaa attctccggc
    20761 tccggccgaa aatggcgcgg tttcgagcag gaggtcgatg gcataacctg tgttcctgcg
    20821 atgaatggaa ggatcgggat actgttcccg gatttcctgc tggttccggg tatccgaaag
    20881 aatatccctg atgttccggt atatatcgcc ttcaagccct tcctgactgt atttaatagt
    20941 gaactccccg tttgtcagcg ggccgaaaac aacttctgtc ccgtcgctta aaaaagccct
    21001 gatttcaagc gtgtggtcgc gggtgctgcc gtaaaccagc gaatgggtgc cgcacgaatt
    21061 gttgccgacc atcccgccga tcatacaccg gtttgaagta gacgtaccgg gcccgaaaaa
    21121 caagcttttc ggttccagca gtttgttgag ttcatccagc acgacgccgg gctcaaccct
    21181 gacccatcct tccgcttcat tgatttcgag gacgcgggtc atatatttag aaacgtctac
    21241 aaccagtccg cggcctacta cctgtccggc cagcgatgtt ccggcgctgc ggggaattac
    21301 cgggatacgg ttctctttgg caaaccggat aatttcagaa atatcttttt tgtctttcgg
    21361 acgcaggacc gccagcggca attcgcgata tgccgacgca tcggtggcat acatcaccag
    21421 ggttgaatcg tcggtgtaaa tgtctcccct gaaatttttt cctaatccgg cgattttttc
    21481 attcagattc atgggtggac ctattcctca acgagttttt tcaggggctt aaaataatag
    21541 ttgtcccagc cgtcgacaat gttatcgaac gcttcatccg gaatgttgat atgcaggact
    21601 tcgacgcggg tttccctgcc ttcgggggcg agggtaatgg ttaccagcga cttttctgcc
    21661 tgcccctcaa aataccattg ctggacgatc ttctggttct cggtgaattc aaggttcagc
    21721 ccggtgatat cgccgtccca gagcgaaaat tcagttcccg ctttctcttc catcacggct
    21781 tccgctcccg accacatctc gatgctgagc gggttggtca acgctttata gacgaattcg
    21841 ggaagcgctt ttatccggta atgttttttc aggtttttca taaacgatgg cgttaagcgg
    21901 cattatatct gtttaataaa taatgcctgt aaccgacatc aaaggtaaag aagttcggct
    21961 attatcggac gcctccatcc tgaacgaagt gtccgggaaa attacggatt ggcagggaag
    22021 gccggttacc ggatcggacc ctgcaatgca ggcgaacttt tttgcgttct gccttctgcg
    22081 tttggcctgc cgtcttaccc acaaccccgc ccaggcaggt aaacttttag ccgttagcta
    22141 ttagccgtta gcctttagct gttggcactg ttccgtcctc cgtcctccat tctccttttg
    22201 acctttgccc tgccgtctta cccacacccg ccacaggcag gtaaactttt gccttttgat
    22261 ctttgtctta tatccaaagg ctgccttagc attactattc cgcaaggagt tttaagcccg
    22321 gagggcttta aacgacgaaa cggtctcccc gccttaccgg aatatatgtc caggctttcg
    22381 ctccctgtcc ctcgcccttt ggcttttacc ttttgccatc tagcttatag ccgttggctc
    22441 cttgcaccaa gcaccatgct ccatgctcct c
//
LOCUS       DAOPYV010000050        22464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_2342_length_22464_cov_12.130706, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000050 DAOPYV010000000
VERSION     DAOPYV010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22464
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2342_length_22464_cov_12.130706"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..663)
                     /locus_tag="PKI35_07770"
     CDS             complement(247..663)
                     /locus_tag="PKI35_07770"
                     /inference="COORDINATES: protein motif:HMM:NF028005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS amidase immunity protein Tai4 family
                     protein"
                     /protein_id="HNX44314.1"
                     /translation="MKKNCLFTLCLLLSCICSAQQNDTPHKEKLSQERLKLKNFAFCQ
                     CLQHVYKNDSVLIIDGSSSGYFETGAYGIDAYNTIDSVANIYSLKVYKSKEGHPLGIM
                     KCLDFYNSNELDKLIQQFDNDIIKQGNNKKRKAIVY"
     gene            complement(676..1677)
                     /locus_tag="PKI35_07775"
     CDS             complement(676..1677)
                     /locus_tag="PKI35_07775"
                     /inference="COORDINATES: protein motif:HMM:NF025478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector amidase Tae4 family protein"
                     /protein_id="HNX44315.1"
                     /translation="MTEVTIIFRQQFLIVVTPDMLVSRLFYEVNFVKPLRMTIPLRDE
                     SGKMPDVFELINMNGRVYDPRIARFLSPDPLLQFPTYSQAFNRYSYCLNNPLKYTDPS
                     GYSLEDGSRKPLSFQERGYTNITLESWFNDGWLSAASDRADAYYRSCFSYDFESNTYV
                     NELGETVDYSLVFENYIAPNCIPLALPLFLDMIKNYPAGQTSEEVYKLIGGKVYQNYK
                     GNPTAYANSCALRISRALNYSGAEIPFVKDQTGSGSDGKWYFYKIKDLKNYLTSTYGP
                     PDLTGNEASMANQHGIILFQDCGWLDATGHIDLWDGNSCAHKCYWDQCGNASLWLLP"
     gene            complement(2176..3525)
                     /gene="gdhA"
                     /locus_tag="PKI35_07780"
     CDS             complement(2176..3525)
                     /gene="gdhA"
                     /locus_tag="PKI35_07780"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNX44316.1"
                     /translation="MSNSIIDQKVDQFMVRVVAKNQGEIEFHQAVKEVVHSLMPFIEE
                     NPKYRGILERMTEPERVIIFRVPWIDDKGDYQVNRGFRIEMNSAIGPYKGGLRFHPTV
                     NLGILKFLAFEQVLKNSLTTLPMGGGKGGSDFDPKGKSDNEVMKFCQSFMTELQRHIG
                     PDTDVPAGDIGVGGREIGYLFGQYKRIRNEFTGVLTGKGLDWGGSLIRPEATGYGCTY
                     FAEEMLKTHGTDFKGKTVVISGSGNVAQYATQKVTQLGGKVVTLSDSNGYVYDPDGID
                     AGKLAWIMELKNIKRGRISEYAKKYNCEYVEGKTPWGVKCDIALPCATQNELNGEDAK
                     ALIANGCICVSEGANMPSTPEAVDIFIEKKILFGPGKAANAGGVATSGLEMSQNSMRL
                     SWTRERVDQELHTIMINIHRTCEKYGKGSDGFINYVKGANVGGFVKVAEAMLAQGLV"
     gene            3919..7395
                     /locus_tag="PKI35_07785"
     CDS             3919..7395
                     /locus_tag="PKI35_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNX44317.1"
                     /translation="MDEDNISKPSRNEIDKLISERTERLKELAAINRTTEILKEGKPP
                     DETLQQLAMIFPAAWQYPDFTVCRITYGGKEYKSPNFTITKWLQRQSFDTIEGRSGYI
                     DIFYLKEFMQYDEGPFLKEERHLIANLATAIAGYLNSITAREILRTSGHTGQLETMTD
                     QAREQPLTGRQLLHRFLNKNNYNRDIYHDLQPFKVREILLVANLYDAYSIEREGRFSE
                     YVLGEYHQLSLSSAPRITGVSTAEEAFEQLNSRHYDLVIFMVGVDKSVAVSLPQEIRK
                     QFPYIPIFTLLNNNTDVEFFTDVRRRYKAIDQIFVWNGESKVFFAMIKMLEDKINVDN
                     DTRVGMVRVILLVEDSPQYYSRYLPLLYGIVMDQTKRIIDDVSSDELYKVLRLRARPK
                     ILLASSHEEALKIINDLKDYMLCLITDVKFERGGKLDPEAGFNLVSYVREVIHDLPVI
                     IQSSEVSNAERAYELRATFIDKYSESLSQEFKSFIAHYLGFGNFIYRNSEGQQIAVAK
                     SLKEFENLLRTIPDESLIYHARKDHFSMWLMARGEIQVAKIINPQKVTDFESPAKLRQ
                     YLIEVIQQFRNEQNKGKIIPFEESAILDEHNVVSLMPGSLGGKGRGLAFINTLIYNYD
                     FNQHVPNINIRSPKTSVIGTDEFEYFIERNKLLDKVLGADDYEEVRRLFISGNLTDTL
                     IRRLRVLLKLIDKPLAVRSSGTFEDSLAQPFAGIFETYLLPNNHPDINVRLRQLMDAI
                     KLVYASVFSPEARGYIEAINYKLEDEKMAVVIQEVVGNQFEDVFYPHISGVAQSYNYY
                     PFGHLTPEDGFAVMALGLGKYVVEGGSAYRFSPAYPAVENYSPKDLYKNSQMKFLAVD
                     LVKGDIDLLEGEMAGLREIDIDEAERHGTLSHLASVYDPESNRMAPGLTHAGPRIINF
                     SDILKYDYAPLAKTIETVLDIVKEALGTPVEIEFAVDLNRDGEYKTTFYLLQIKPLIG
                     AEQDYQIDLNEIPEDEIILLSFKGMGNGVINDIQDVIYIDPERFDKSRTVEMAKEIEI
                     LNHKMRAEKRRYVLIGPGRWGTRDRWIGIPVNWPQISNAKVIVETSLEGYPLDASSGS
                     HFFHNVTSMNVGYFTVQPEVDHSFVRYTFLRRQELIEKTAFFNHVRLQKPLNVKMDGR
                     KRLAVITRNGKL"
     gene            complement(7505..8872)
                     /gene="sprA"
                     /locus_tag="PKI35_07790"
     CDS             complement(7505..8872)
                     /gene="sprA"
                     /locus_tag="PKI35_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNX44318.1"
                     /translation="MKSAITLLSAILLLSLFTASCKKETNGDDSTTKTSDLRSVSAWH
                     FASVPLRFPESELTNDKACGNNRAKIAWYVIDPLFFDTNSQLRPGNISSDDISEDDCR
                     AIMNYEAYPTIKNTAEPMNIAVFEINYYPSERGPWNFDLNASSYSAGISHDGTLNDPA
                     SRWGGIERPLPESSDRMNYLEFCLLDPFTICPDASGEMYIDIGDISEDILKDGVLSAE
                     NTTEGAFQTTAWGLAKPLTGNACFPASGIEPYDTGLDEIADKDERIYFKSYLDEISSL
                     CAPQVYESIQNDPSNDNYHYFRGNDYDQLNYKVCARYNYYNNQEKNSVSSTNTSYCTI
                     SNSVPESEDMNRNGILDTPDNYFEYKISINKQAFEVGSNYIVNMLHNYNVKLANGNTT
                     ESKFYHFRIPLANSNAQYGSPDISSNPKSIRIYLTGFSAPVRLRFISLGLSREPIQFE
                     GWDYQ"
     gene            complement(8957..10405)
                     /locus_tag="PKI35_07795"
     CDS             complement(8957..10405)
                     /locus_tag="PKI35_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNX44319.1"
                     /translation="METITKLIEFTSEISILELSYIVASVLFILGLKKLSHPATARAG
                     NLWSAAGMTAAIIFTILFHKKDGEPIGNIVWIVVALAIGSFIGWYASKKVKMTAMPQI
                     VSIFNGMGGACAALISMMEFPKMQAALAIETAPHMLCGEAIAILLGLMIGSVSFAGSM
                     IAFLKLDGRIGDIKNPVLRIINLTFLVVLLVALVLIMFLQPVEGNNWPIYVFACAALL
                     YGVTFVMPIGGADMPVVISLLNSFTGVAAAMGGFLYNNQAMLTGGILVGSSGTILTIL
                     MCKAMNRSLVNVIIGAFGVSAAGGSEAADKTVKEISLSDAAVLLSYSQKIYIVPGYGL
                     AVAQAQHLCHELDNLLSSKGVEVKYAIHPVAGRMPGHMNVLLAEADVPYEKLVEMEDA
                     NNEMANTDVAIVIGANDVVNPAAEEDPSSPIYGMPIIKVLDARNIIVMKRSMAAGYAA
                     IPNNLFYHPKNRMLFGDAKVTLQKLVAEIKSL"
     gene            complement(10421..10723)
                     /locus_tag="PKI35_07800"
     CDS             complement(10421..10723)
                     /locus_tag="PKI35_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNX44320.1"
                     /translation="MEEILKFFMEYKDMIFIIILSIFLGIEVISHVPAVLHTPLMSGS
                     NAIHGVVIIGAIIVMGHAEDTLPLILGFIAVILGTLNVVGGFVVTDRMLEMFRKKK"
     gene            complement(10736..11863)
                     /locus_tag="PKI35_07805"
     CDS             complement(10736..11863)
                     /locus_tag="PKI35_07805"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNX44321.1"
                     /translation="MTIGILKENADENRVAMLPESVSTLVKMNVTMLVETGAGLAAFA
                     ADADYEKAGAKVLKKAEVISQSDVLIKIQPPTAEETALLKEGQVLLAVLNPYFNGNLV
                     KELAAKDITAFSMDVVPRTTRAQAMDILSSMATVAGYKAVLTAANTLPKFFPMFMTAA
                     GTIKPANVLILGAGVAGLQAIATSRKLGAVVYVFDVRAAVKEEVMGLGGKFVEVEGAT
                     DDKSAGGYAVEQTEEFKMRQAKAIHDQAAKSDVVICTAQIPGKKAPVLLKKETIEAMT
                     PGSVIIDIAASTGGNCELTRNNETVIHNGVKIIGNSQLPTEMPTDASRMFGKNLINFL
                     KLIITKEGALNLNWDDDIVKGTAVTHGKEIVHEKVKSIINS"
     gene            12210..15899
                     /locus_tag="PKI35_07810"
     CDS             12210..15899
                     /locus_tag="PKI35_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HNX44322.1"
                     /translation="MKKRLLLLIAIAITFISFSTASVVRNEPYQVQQPDGAIINCFIS
                     GDEYFNWLHDADGYTIIQAADGYYYYGIRQGDIVVPSSYKVSEVNPESVGLRKWAKIS
                     NAEYRRIKNNQLTDGGASVTAPHTGTMNNIAVYIKFSDDSEFTTTRQTYDDLFNMPTG
                     VSLKSYYTEVSYNLFTISSTHYPACAMTTNLSYTDSHPRSYFQPYNATTNPNGYSNDD
                     QRTSREHALLRDAVNWINANSPVPAGLNIDGDNDGYVDNVCFIVRGDHGAWASLLWAH
                     RWVLYSYNVSINGKQVWDYTFQPETQVDVTTLCHEMFHALGSPDLYHYDDGGLNIAPV
                     ADWDLMESGGGHMTAYMKWKYTDQAWISSIPEITTSGTYTLQPLTSSTNNCYKIPSPN
                     SSSEFYVVEYRKATGTFEGNLPGSGLIVYRIDPTVSGNADGPPDEVYIYRPNGTTTTN
                     GSPNSAYYSSTSGRTAINDGTNPSPFLQDGSAGGLNLYNVTSAGTTISFTVGMSNVQN
                     PSAFTATAVSTTQIDLSWQKNSSNNNVMLVYNTTSAIGNPVAGTSYPAGSAIPGGGTV
                     IYNGSGTTFSHTALTPSTTYFYKLWSVDGTNNYSNGVSNNATTLCGIATLPYTESFTN
                     STFPACWGTHTTGSSTTNSWEPSGTSNAGGTACEMKSTYQEVNPGATRLVSPSFNTTG
                     ITQLNLSFRFLLDDWAPGAVMKVQSSTDGVNWTDEAWSLATSSNAAVGPYLVNTTVTS
                     NLNSTTTYIAFTAEGDLYQYDYWYIDDVSIQNGGSQSYLITTASNPVSGGSTSGGGTY
                     QIGQNVTVIATPAANWNFINWTAGGTVVSAEPTYTFTASANLDLVANFSMNQVSVSTT
                     ASPASGGTTTGDGTYLIGDPVTVTATPNVGYEFVNWTENGNIVSTNVVYAFTASASVN
                     LVANFSVLQYTVSVSASPVAGGTVSGGGTYSYNASVSVVASANSGWDFIGWFENGTMV
                     SGNAAYSFNILENHVLEAHFVQQIPVYTITVSANPPEGGTVSGSGSAASGSQVTVTAA
                     ASQGWVFTNWSENGNVVSTNSEYTFTITGNVSLVATFAYVYNITASVSPANTGYITGD
                     GPYASGSTATLNAYPFLGYQFVNWTLDGNVVSTNPEYSFTVVSDDHFVANFQTEVGVS
                     DHGKQSFRVYPNPTSGYLTIESSAGGFEKITLLNQVGKSVMMVNPDGSGQKIVLNLSG
                     LNPGIYALQLKFRNGTASTTKVVIK"
     gene            complement(15987..17003)
                     /locus_tag="PKI35_07815"
     CDS             complement(15987..17003)
                     /locus_tag="PKI35_07815"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNX44323.1"
                     /translation="MIDLRSDTVTRPTPGMLDAMMHAAVGDDVFGEDPTVNDLENYVA
                     GIFGKEAALFCTSGTQSNQVGIKAHTQPGDEVICDHLAHIYYYEGGGIAFHSGCSVCL
                     LDGDRGRITAEQVKGHINPTEDPHRPLTRLVCVENTMNKGGGAVYDFNELIAIQKVCR
                     ENNLMYHLDGARLFNAIVETGKTPADFGQLFDSISICLSKGLGAPVGSVLIGNKEFIR
                     KARRIRKVFGGGMRQAGFLAAAGKYALLNNIQRLKEDHARARRIGEMLRNLPFVNEIV
                     PIDTNMIFFHLDNSMPSDDFLEKLRQKDIHAMALSPQVIRFVTHLDITDPMLEILEME
                     LKKL"
     gene            complement(17067..17369)
                     /locus_tag="PKI35_07820"
     CDS             complement(17067..17369)
                     /locus_tag="PKI35_07820"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNX44324.1"
                     /translation="MIRHIVLFKLRDFESEIRKQEIRLKIKNRIEELPGKIDLIRRCE
                     VGVDIRKLEMSYDIVLIMDFDNLNDLNTYTDHPAHQEFIRFNKEFSAAKVCIDCEI"
     gene            complement(17382..18548)
                     /locus_tag="PKI35_07825"
     CDS             complement(17382..18548)
                     /locus_tag="PKI35_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNX44325.1"
                     /translation="MTFNFDEPVDRLHTDSLKYDGLTQFFGSADLLPMWVADMDFKTP
                     QFIIDALKKRLQHEVMGYSLRPQHFLDAIKGWVKRRHNWDISTDWIVFTPGIVPAVNI
                     SVLAYTSVSDGIITQPPVYFPFFNAATDHRRKLIYSPLKLVNGRLSIDFTDLEQKCRQ
                     GARMIILSSPHNPGGNVWTRDELSRIAEICLRHNILVVSDEIHSDLVYKPHVHTPMAS
                     LSDEIAANTVTFIAPSKTFNVSGLSSSAAIIPDKKLREQFVATLESLHVGLGNIFGNV
                     AMEAAFTHGDKYVDALMDYLAGNVSLVESFLSENIPQIKPVRPEATFLMWLDCRELGM
                     TDVELKDFFLNKAHLALNQGIQFGPGGEGFMRMNIGCTREVLRKALNQMKEAFI"
     gene            18668..21673
                     /locus_tag="PKI35_07830"
     CDS             18668..21673
                     /locus_tag="PKI35_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNX44326.1"
                     /translation="MKPSQLALLSQKYYFDDTPFINLMKKRIYHVLLIASNYDAFILE
                     GDGRIDEQIFNEYVSLNLRYPPQFIQVPSEKEAFKTLREEKIDLVICMLNLEELDTFE
                     LARKIKSEHPKIPIVVLTPFSREVSLKVTHEDLSAIDYVFSWLGNSDLLLAIIKLIED
                     KMNAEHDTSEVGVQVVLLVEDSIRFYSSYLPNIYKIVFNQSRSFMTEGLNEHQQMLRN
                     RGRPKILLATNYEEAIDLYEKYRNNLLGIISDINYRRKGKEDPEAGFRLLRHVRGKDE
                     FVPFLLQSSDEKYASVAREMNVGFINKNSKTLSIELRHFMMEYFAFGDFVFLNPETHE
                     EIMRATDLKALQQRIFEVPDESLLYHIYRNHLSKWLFARALFPLAEFFKDVRAGDFTD
                     LDEIRRFLFEAIAGYRLNKARGVIAEFNRDSFDEYVTFTRIGDGSIGGKARGLAFLDT
                     IIKRNRLFDKFPGVNISIPHTVVLCTDVFDEFMEDNDLYKIGLSDIPDEEILNQFINA
                     RLPFRLHDDLGKFISVSRRPIAVRSSSLLEDSHYQPFAGIYSTYMVPQVSGDDRLMLE
                     QLSNAIKSVYASVFYHNSKAYMAATRNLIDEEKMAIVLQQVVGSHFGGTFFPTFSGVA
                     RSINYYPLAPEKSEDGIASIAIGLGKYIVDGGTSLRFSPRYPTKVLQLSSPEMAVRET
                     QKHFYALDLKPGSFWPSLDDGMNMKHLKIEDASEDMAFKDMVSTFDFDNNVLREGVNI
                     EGRRVVTFANILNHNSFPLAEILRICLEMGHRELGKPIEIEFAVDLHRPKSTPKAFTL
                     LQIRPIVESKESNDVKIGDVKTENALIYSNAALGNGLITGIEDLVYVKPEAFDPAQNS
                     GTATRIGELNDKFLASQKNYILVGPGRWGSTDPWLGIPVKWPQISAARLIVESGLENY
                     RIDPSQGTHFFQNLTSFRVGYFTINPFMSDGFIDLELMNSLSAIFEDDIIRHVRFNQP
                     LTIKIDGRHNKGVVLKPSPQTEDDQDENN"
     gene            21785..22351
                     /locus_tag="PKI35_07835"
     CDS             21785..22351
                     /locus_tag="PKI35_07835"
                     /inference="COORDINATES: protein motif:HMM:NF040058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44327.1"
                     /translation="MKKLNQWTILLLLPVVILAGCKNKKADEKSLITRRIQYDVPIKA
                     SDADNDWWVQNIEGQDREKLVKEIMESALSGKVKTYDFFSNELLTPDQLKAIMKRSDT
                     ITLERAAPPHSLYDTVLTKELSLRDITRLRFLEEWNMDKKTLQFNKKVAGICPLIANY
                     GDSAELRGYMPLFWVFFDDKYPGELKMK"
BASE COUNT         5854 a         5285 c         5386 g         5939 t
ORIGIN      
        1 gggttagtct gcaaaatggc tgacccggtt atattttttt accggacaac agtgattgaa
       61 attatcataa aggtccgctt ttcgctattc ctttaaaaat gttcattacc gtaagcttgc
      121 tattaaagat atatcgtcag ataaaatgtt ataggatttc tttcaataga acctagccgc
      181 ccaagaaaaa gaaaaactca ccgtttattt aagttgctta tctcgttaaa ccacaaatca
      241 gctatgtcag tatactattg cttttctttt tttattattt ccttgtttaa taatgtcgtt
      301 atcaaattgc tggattaatt tgtcaagttc attactatta taaaaatcaa ggcatttcat
      361 gattcctaat ggatgacctt ccttgctttt gtaaactttc agactataaa tatttgcaac
      421 actatctata gtgttataag catcaatgcc gtaagctcct gtttcaaaat atccgcttga
      481 actaccatct ataataagaa cggaatcatt tttatagaca tgttgtaaac attgacaaaa
      541 tgcaaagttt tttaatttta gtctctcttg tgataacttt tctttgtgcg gagtgtcatt
      601 ttgctgtgca ctgcaaatac agcttaacaa aagacataat gtaaacaaac aatttttttt
      661 catagttgcg tgattttaag gtaataacca taaagatgca ttcccgcatt gatcccaata
      721 acacttatga gcgcaactgt taccatccca aaggtctata tgcccagtag catccagcca
      781 cccgcaatct tgaaaaagga taatgccatg ttggttcgcc attgaggctt cattaccagt
      841 caagtcgggg gggccgtatg tactagttag gtagtttttt aaatccttga tcttataaaa
      901 ataccacttg ccgtcggaac cagatccggt ttgatcttta acgaaaggaa tttctgcacc
      961 actataatta agggctcgac ttatccttaa agcgcaagaa tttgcgtacg cagtaggatt
     1021 gcctttataa ttctgatata ccttgccccc aattagttta taaacttctt ctgatgtttg
     1081 tcctgctgga taatttttaa tcatatcaag aaataaaggt aatgctaatg ggatgcaatt
     1141 gggggcaata taattttcaa acactaaact ataatcaaca gtttcaccaa gctcgttaac
     1201 ataggtgttg ctctcaaagt cataagaaaa gcagcttcta taatatgcat cagccctatc
     1261 actcgcggcg cttaaccatc catcattaaa ccatgattcc agcgttatat tggtataacc
     1321 tctttcctga aaactgagcg gtttacggga tccatcctcc aatgaatatc cgctcgggtc
     1381 ggtatatttc aacggattgt tcaaacaata actgtaccgg ttaaaagcct gggagtatgt
     1441 tggaaactga agtaacggat caggagataa aaacctggct atcctcggat catataccct
     1501 gccattcatg ttgataagtt caaaaacatc cggcattttc ccgctttcgt ccctcagcgg
     1561 gattgtcatc cgtaaaggtt ttacgaagtt cacttcgtaa aacaaacggc tgactaacat
     1621 gtccggtgta accacgatca aaaattgttg acggaatatt attgtaactt cagtcatttg
     1681 ggttgcgcct tcggccccag ggatcataac taaggtgctc cagcttattt ccttccccgt
     1741 cgcttattac ctggtaagaa ttctggtagt ctttaggaat attgaagatt caaagatata
     1801 cagtttctgc aaatatcaaa caaccgtctg ccgaaggcgg atgaggtgat gataaaaagg
     1861 aacttcctca ggaaaacaga ataaagtaag aagcctctta cgtcttcgta tcattcgcag
     1921 ctttggccgg ttttcgcccg gaaataccgg catggtttgt tccgaagttt cagtccggtc
     1981 ccggctgctt tttttagaga tgctcaccgc tgtatgtttg cgcggttgtt accatacgcc
     2041 agaccctgaa ggtttttaaa accttcaggg tctgttttgt gtgcgtacga acgggaggag
     2101 atgggatcta tttctatttt ttgaatttct ccgttcattt ccaacggaac attccctgcg
     2161 ggatgatcac accatctaaa ccagtccctg ggccagcatg gcttcggcaa cttttacgaa
     2221 gccgcccacg ttggcgccct tcacatagtt gatgaatccg tcgcttccct tgccgtattt
     2281 ttcgcaggta cggtgtatat tgatcatgat ggtgtgcagt tcctggtcaa cgcgctcacg
     2341 ggtccagctc aggcgcatgc tgttttgcga catctcaaga cccgacgtgg ctacgcctcc
     2401 ggcgttggcg gctttgccgg ggccaaacag tattttcttt tcgatgaaaa tatccacggc
     2461 ttcgggggtt gacggcatgt tggcgccttc cgaaacgcag atacaaccgt tggcaatcag
     2521 ggcttttgca tcctcgccgt tcaattcgtt ctgagtggcg cagggcaggg caatatcgca
     2581 tttcacgccc cacggggttt taccttccac atattcgcaa ttgtactttt tggcatattc
     2641 gctgatacgg ccgcgcttaa tattcttcag ttccatgatc caggccagtt tgccggcatc
     2701 aataccgtca gggtcataga catagccgtt tgaatcgctg agggtgacaa ctttcccgcc
     2761 aagctgggtt actttttggg ttgcatactg ggccacattc cctgatccgg aaataacaac
     2821 cgttttgcct ttaaagtccg tgccgtgggt tttcagcatt tcttcggcaa aataagtgca
     2881 gccgtagccg gtagcttcgg gacggatcag gctgccgccc cagtcaaggc cttttccggt
     2941 aagcactccg gtgaattcat tgcggatgcg tttgtactgc ccgaacaggt aaccgatttc
     3001 ccgtccgcca accccgatgt cacctgcagg aacgtcggtg tcggggccga tatggcgctg
     3061 taattcggtc atgaacgact ggcagaattt catgacttcg ttatcgctct ttcctttcgg
     3121 atcaaagtcg gagccgcctt taccgccacc catgggaagt gttgtcaggc tgtttttcag
     3181 tacctgctcg aaagcaagaa atttcaggat gcccaggttt acggtcgggt gaaagcggag
     3241 gccgccttta taagggccga tggcgctgtt catctcaata cgaaaaccac ggtttacctg
     3301 gtaatcgcct ttatcgtcga tccagggaac ccggaaaata ataacacgtt cgggttctgt
     3361 catgcgttcc agaatacccc ggtatttcgg gttttcttcg atgaaaggca taagcgaatg
     3421 aaccacttct ttaactgcct ggtgaaattc gatctcaccc tggtttttag cgacgaccct
     3481 gaccatgaat tggtcaactt tctggtcaat aatgctgttt gacataggtt tctgatttgg
     3541 ttagtaaaac agagatgaac atgattttgt acaacaatat tactcatatt actatacatg
     3601 gcaataaaaa atacgcttta tttattttat ttcagtcgat gtaaaatatt gaaatataat
     3661 aaaatatgaa gcgataagta aaatacggat ataattcagc aaaacactga aagactcttt
     3721 tctgccattg tgatagaata gaatcttagt aggatataca agcccgggga cactttttac
     3781 atctggtaag aacaaaaaaa atggccggag catagcggtc atcagtggca cactgcgata
     3841 aaatccggtc ggtgtgcttc cctgtaatca gctatttcat tacttttata ccatacaaca
     3901 caggtgaaat ctgctgacat ggacgaagat aacatttcaa agcccagccg gaatgaaatt
     3961 gacaaactga tttccgagcg taccgaaagg ctcaaggaac tggctgccat taaccgtact
     4021 accgaaattc tcaaggaagg taaacctccg gatgaaaccc ttcagcagtt ggctatgata
     4081 tttcctgcag catggcaata tcctgatttc acggtctgtc gcattacata tggcggtaag
     4141 gaatacaaat cgccaaattt taccattacc aaatggctgc aacggcagtc attcgatacc
     4201 attgaaggca gaagcggcta tatcgatatt ttctatctga aggaatttat gcaatacgac
     4261 gaaggtccgt tccttaaaga agaacggcac cttattgcca atcttgctac ggctatcgcc
     4321 ggttacctga acagcatcac ggccagggaa atcctcagaa catcaggcca taccggtcag
     4381 ttggaaacca tgaccgatca ggccagggag cagcctctga caggccgcca gctcctgcac
     4441 cgcttcctca acaagaataa ttataaccgc gatatttatc acgaccttca accgtttaaa
     4501 gtgagggaga tactgctggt cgccaatctt tacgacgcct attccattga acgcgaaggc
     4561 cgtttctcgg aatatgtgct tggagaatac caccagctaa gcctcagttc ggctccgcgg
     4621 atcacgggcg tttctacggc cgaagaagct tttgagcaat tgaattcaag gcattatgac
     4681 ctcgttatct ttatggttgg cgttgataaa tcggtggccg tcagccttcc ccaggaaatc
     4741 cggaagcaat ttccgtatat tcccatcttt acgctgctca acaataatac cgatgtcgag
     4801 tttttcaccg atgtgcgtcg ccgatacaag gctattgacc agatatttgt gtggaacggt
     4861 gaaagcaagg tgttttttgc catgatcaag atgctggagg acaagatcaa tgtggataat
     4921 gatacgcggg taggcatggt ccgcgtgatc ctcctggttg aggacagccc tcagtattac
     4981 tcgcgctatc tgccgttgct gtatggaatt gtgatggacc agaccaagcg gatcatagat
     5041 gatgtaagtt ccgatgaact ttacaaagtg ctgaggttga gggcccgtcc caagatttta
     5101 ctggcatcga gccatgaaga ggctttaaag attatcaacg acctgaagga ttacatgctg
     5161 tgccttatca cggatgtaaa atttgagcgc ggcgggaaac tggatcctga agcgggtttc
     5221 aatctggtaa gctatgtccg tgaagtgatc catgacctgc cggtgatcat ccagtcgtcg
     5281 gaagtttcaa atgcggaacg ggcatacgag ttgcgcgcca catttattga taaatattcc
     5341 gaaagccttt cccaggaatt caagagcttt atcgcgcatt atctgggttt cgggaatttt
     5401 atttaccgga acagcgaagg acaacagatc gccgttgcca aatcgctgaa ggaatttgaa
     5461 aacctgctgc gcactattcc cgacgaatcg ctcatatacc atgcccgcaa ggaccatttc
     5521 tcgatgtggc tgatggctcg tggcgaaata caggttgcca aaatcattaa tccgcaaaaa
     5581 gttaccgatt tcgaaagccc tgccaaactg aggcaatacc ttatcgaggt catccagcag
     5641 ttccggaacg agcaaaataa gggcaagatc attccattcg aggaatcggc aattcttgac
     5701 gagcataatg tcgtaagcct gatgccgggt tcattgggcg gcaaaggccg gggcctggca
     5761 ttcatcaaca cactcattta caattatgat ttcaaccagc acgtgccgaa catcaatatc
     5821 cgctcgccga aaacatcggt aatcggaacc gatgaatttg aatacttcat agagcgtaac
     5881 aagctgttgg acaaagtgct gggcgctgac gattatgaag aagtgcgccg attgttcatc
     5941 agcggcaatc ttacggatac gctcatccga agattgcgcg tgttgttgaa acttattgat
     6001 aaaccgctgg cagtgcgctc atcgggcact tttgaagatt cgttggcaca gccgtttgca
     6061 ggtatttttg aaacctatct gctgccgaac aatcacccgg atataaatgt gaggctcagg
     6121 caactcatgg atgccatcaa actggtatat gcttcagtgt tctctcccga agccaggggg
     6181 tacattgaag ctatcaatta caaactggaa gatgagaaga tggcggttgt gatccaggag
     6241 gtggtcggga accagtttga ggatgttttc tatccgcaca tcagcggagt ggcccaatcc
     6301 tataattatt atcccttcgg acacctgacg cctgaagacg gctttgccgt gatggcgctc
     6361 ggacttggta aatatgtggt tgaaggggga agcgcttatc gcttttcacc ggcatatccg
     6421 gccgttgaaa actattcccc aaaagatttg tacaagaact cccaaatgaa atttctggca
     6481 gtcgatctgg tcaaagggga tatagacctg ctggaaggcg aaatggccgg gcttcgcgaa
     6541 attgacattg atgaagccga gcggcacgga accttaagcc acctggcgtc ggtatatgat
     6601 cccgagagta accgcatggc accgggcctt acccatgccg ggccacggat catcaatttt
     6661 tcggatatcc tgaaatatga ttatgcaccc ctggccaaaa ccatcgaaac agtgctggat
     6721 atcgtgaagg aagcgttggg aacgccggtc gaaatagagt ttgccgttga cctgaaccgc
     6781 gacggggaat ataaaaccac gttttacctg ttacagatca agccgctgat aggcgctgaa
     6841 caggattacc agatcgacct gaacgagatc ccggaagatg aaatcatcct gctttcattt
     6901 aaaggtatgg gcaacggggt aatcaatgac atccaggatg tgatctatat tgacccggag
     6961 aggttcgata aaagccggac cgtggaaatg gcgaaggaga tagaaattct gaatcataag
     7021 atgcgtgccg aaaaacgacg ctatgtcctt atcggccccg ggcgatgggg cacccgcgac
     7081 cgctggatcg gaattcccgt aaactggccg cagataagca atgccaaggt gattgtggaa
     7141 accagcctcg aaggctatcc gctcgatgca tcgtcgggat cgcacttttt ccacaacgta
     7201 acttcgatga acgtggggta cttcaccgta cagcccgaag tggaccatag ttttgtccgc
     7261 tatactttcc tccgccggca ggaactcatc gaaaagaccg ctttttttaa tcatgtgagg
     7321 ttacagaaac cgttgaatgt gaaaatggat ggcaggaaac ggctggctgt aatcacacgg
     7381 aacgggaaat tgtgaacttt tccgtcagca ggcggttttt taaccggcaa atagcagaag
     7441 cgcagcgatt gactggcgtc agccaagagt gaagaaatat gcggcccgga tttgatccgt
     7501 tcctttactg gtaatcccat ccctcaaatt ggatgggttc ccggcttaaa cccaggctta
     7561 taaacctcag cctgacgggc gccgaaaatc ctgtaaggta aattcttata gacttcggat
     7621 ttgatgaaat atcgggagaa ccatattgtg cattagaatt cgctaaaggt atccggaaat
     7681 gataaaattt cgattcggtg gtattgccgt ttgccagctt gacattatag ttgtgcaaca
     7741 tattgacaat gtaatttgag ccgacttcaa atgcctgttt gttgattgaa attttgtact
     7801 caaaataatt gtcgggcgtg tcgaggatcc cgtttcggtt catatcttcg gattcaggaa
     7861 cactgtttga aatggtgcag taagaagtat ttgtgctgct aaccgaattc ttttcctgat
     7921 tgttataata attgtatcgg gcgcatacct tgtaatttag ctgatcataa tcgttgcccc
     7981 ggaaatagtg atagttgtcg ttcgacggat cattctgtat cgattcataa acctggggtg
     8041 cacataaaga actgatttcg tccaggtaag atttaaagta gattctttcg tctttatctg
     8101 ctatttcatc caggcctgtg tcgtagggtt ctatgccgga agccggaaaa caggcatttc
     8161 cggtcagagg tttggctagt ccccatgccg tggtttgaaa ggcgccttct gttgtatttt
     8221 cggcagataa caccccgtct tttaaaatgt cttcgctgat gtcgcctata tcgatgtaca
     8281 tctctccgct tgcatcaggg cagatggtaa aaggatcaag aagacagaat tccaggtagt
     8341 tcatcctgtc ggaggattcc ggcaatggtc tctcaattcc gccccatcgg gaggctgggt
     8401 cgtttaaagt tccgtcatgg cttataccgg cagaatatga agatgcgtta aggtcaaaat
     8461 tccagggacc tctttcggaa gggtagtaat taatttcaaa gaccgcgata ttcataggtt
     8521 cagccgtatt tttgatagtc ggataagctt cataattcat tatggccctg cagtcatcct
     8581 ccgatatatc atcggatgaa atgttcccgg gccttaattg ggagttggtg tcaaagaata
     8641 aaggatcaat gacataccag gctatttttg cccggttgtt accgcaagcc ttgtcatttg
     8701 tcagttctga ttcagggaac cggagcggga cagaggcaaa atgccatgcg ctgacgcttc
     8761 tcaggtccga agttttcgta gtggaatcat ccccattggt ttccttttta caagatgcag
     8821 taaataatga aagtaacaga atggcggata gtaatgtaat cgcagatttc ataatatgag
     8881 taaattaaaa aaacaaatat ataaaaagcc ggcaaaaatt gttcttctgc cggctttaat
     8941 taaataaatt acagatttac aggctcttga tttcagccac cagcttttgt aatgtcacct
     9001 tagcatcgcc gaagagcatg cggtttttcg ggtgatagaa aaggttgttg gggatagcgg
     9061 cataaccggc agccatactg cgtttcataa caatgatgtt acgggcgtcc agcaccttta
     9121 tgatgggcat accgtaaatc gggctcgaag gatcttcttc ggcggcggga tttaccacgt
     9181 cgttggcgcc gatgacgata gctacatcgg tattggccat ttcgttgttg gcgtcttcca
     9241 tctccaccag cttttcataa gggacatcgg cttcggcaag caacacgttc atgtgccccg
     9301 gcatacgtcc ggcaacaggg tggatggcat attttacttc aacgcctttt gacgaaagca
     9361 ggttatcaag ttcgtggcag aggtgctgtg cctgggcgac ggcaaggccg taacccggga
     9421 caatataaat tttctgggaa tagctgagca gcacggccgc gtcgctcagc gatatttcct
     9481 ttaccgtttt atcggcagct tcggaaccgc cggcggcgga tactccgaat gccccgatga
     9541 taacgttgac cagcgaacgg ttcatggctt tgcacatgag tatggtaagg atggttcccg
     9601 atgaacctac caggataccg ccggtaagca tggcctggtt gttgtacagg aaaccgccca
     9661 tggctgcggc aaccccggtg aaagagttga ggagggaaat tacaacgggc atgtcggcgc
     9721 cgccgatagg cattacaaag gttacaccgt agagcagggc agcacaggca aatacataaa
     9781 tgggccagtt gttcccttca acaggttgca ggaacatgat caggacaagc gcaacgagca
     9841 gcactaccaa aaaggtcagg ttaatgatgc gtaatacggg gtttttgata tcgccgattc
     9901 ttccgtcgag tttcaggaag gcgatcatac tccctgcaaa cgaaacgctg ccgatcatta
     9961 gtccgagtaa aatggcaata gcttcgccgc ataacatatg aggagcggtt tcaatggcga
    10021 gggcagcctg cattttcggg aattccatca tactgatcag ggctgcacaa gcgccgccca
    10081 tcccgttaaa gatggagact atctgcggca tagccgtcat tttgactttc tttgaagcat
    10141 accacccgat gaaggaaccg atggcaagcg ccacgactat ccatacgata ttgccgatag
    10201 gttcgccgtc ttttttatgg aagagaatgg tgaaaataat cgcggcggtc ataccggcgg
    10261 cagaccagag gttgccggcc cgcgccgtgg ctggatgact gagcttctta aggcccagga
    10321 tgaaaaggac ggaggcgacg atatacgaaa gttcgagaat cgaaatttcg ctggtgaact
    10381 caatgagttt tgtaatggtt tccatattct gcctcccgtg ttatttcttt ttcctaaaca
    10441 tttcgagcat acggtcggtg actacgaaac cgccgacaac attaagcgta ccgagtatca
    10501 cggcgataaa accgaggata agcggcaggg tatcttcggc atgtcccatc acaatgatgg
    10561 cgccgatgat aacgactccg tgaatggcat tgctgcccga catcagaggc gtgtgcagca
    10621 cggcgggcac atgcgatatt acttctatcc cgaggaatat ggaaaggata atgatgaaaa
    10681 tcatatcctt gtattccatg aagaatttta aaatttcttc cataagtatt tctggttatg
    10741 aattgataat tgatttcact ttttcgtgta cgatttcttt cccgtgggtg acggctgtgc
    10801 ctttaacaat atcgtcgtcc cagttcaggt tcagggcgcc ttccttcgtg atgatgagtt
    10861 tcaggaaatt gataaggttt ttgccgaaca tacggctggc gtcggtaggc atttcggtgg
    10921 gaagctgcga gtttccgata atttttacgc cgttatgaat aaccgtttcg ttgtttctgg
    10981 taagttcgca gttgcctcct gttgaagcgg caatatcaat gatcaccgat ccgggtgtca
    11041 tggcttcaat ggtttctttt ttaagcaata ccggggcttt tttaccgggg atctgtgctg
    11101 tgcagattac gacatccgat tttgctgcct ggtcatgaat ggcttttgcc tggcgcattt
    11161 taaattcctc ggtctgttca acggcatagc cgccggccga tttatcatcg gtagcgcctt
    11221 caacttccac gaattttccg ccaaggccca tcacctcttc ttttacggcg gcgcgaacat
    11281 cgaaaacata aaccacggcg cccagcttgc gcgaggtggc gatagcctgt aaccctgcga
    11341 cgccggcccc caggatgagc acattggcag gcttaatggt gcctgcagcc gtcatgaaca
    11401 tggggaagaa cttgggcagt gtattggcgg cggtcagtac ggccttgtag ccggccaccg
    11461 tagccatgga agaaaggata tccatagcct gcgccctggt tgtgcggggt actacatcca
    11521 tgctgaaagc cgtaatgtcc ttagctgcaa gttcttttac caggttgcca ttaaaataag
    11581 ggttcaggac agccagcaac acctgacctt ccttcagcaa cgctgtttct tctgccgtcg
    11641 ggggttgaat cttaattaat acatcagatt gggagataac ttcggctttc ttcagaactt
    11701 tagcgccggc tttctcgtag tcagcatcgg ctgcaaaggc agctaagccg gctcccgtct
    11761 ctaccagcat ggtcacattc atctttacga gtgtggatac actttcggga agcatggcta
    11821 cccggttttc gtcagcattc tctttgagaa ttccgattgt cataatggga ggttttaaaa
    11881 aattcgggca aatgtacggg aaaatttccc agacgaaagt atgtcattgg ccaatatttg
    11941 aaaactttca gtaaatcact gattttattt gcattctttt tgtgaaatga ttaacaaatg
    12001 aaatactcag ggagatatta ccggatgcgt ttttcaaaca agttgccggt aattgacatt
    12061 aaaggaatta aaacaaaaat tttttcctaa agacgagtta cttttgtgca taaatagcaa
    12121 ttaattctag ggattggaat gatttctcaa acgaatgccc ctggtaataa aagtaaaaaa
    12181 atcaaaacta tttcagcaac aattgtttta tgaaaaaacg tctactgctt cttattgcaa
    12241 ttgcaataac attcatttca ttttcaaccg cgtcggttgt aagaaacgag ccataccagg
    12301 ttcagcaacc tgacggggcc atcattaact gttttatttc gggtgatgaa tatttcaact
    12361 ggctgcatga tgccgatggc tatacgatca tccaggcagc cgatggatat tattattacg
    12421 ggatcaggca gggcgatatt gtggttccgt cgtcgtataa agttagcgag gtcaacccgg
    12481 aatcagtcgg cttacgcaaa tgggccaaaa tctccaatgc tgaatacagg cgcattaaaa
    12541 acaatcagtt gaccgacgga ggcgcttcgg tcacggcgcc gcataccgga accatgaaca
    12601 acattgcagt ttatattaaa ttttcggatg attcggagtt tacaacaacc cgccagacct
    12661 acgatgattt gtttaacatg ccgaccggcg tttcactcaa atcatattat accgaggtat
    12721 cgtacaacct tttcacgata agttcgacgc attatccggc ctgcgccatg acgacgaacc
    12781 tttcgtatac cgattcgcac ccccgtagct attttcagcc ttacaatgca acaaccaacc
    12841 cgaacggata tagtaatgac gaccaacgta cctcccgtga acatgcattg ctgcgcgatg
    12901 ccgtaaactg gatcaatgca aattctccgg tacctgccgg cctgaatatt gacggcgata
    12961 acgatggcta tgtggataat gtctgtttta ttgtaagggg cgatcacggc gcctgggcaa
    13021 gcctgctgtg ggcgcatcgc tgggtgctct attcctataa cgtgtctatt aacggtaaac
    13081 aggtctggga ttacacgttt cagcccgaaa cgcaggtcga cgttacgaca ttgtgccatg
    13141 aaatgtttca tgcccttggc tcccccgatc tttaccatta tgatgatggc gggctgaaca
    13201 ttgcaccggt tgccgattgg gacctgatgg aatcgggcgg cggccatatg acggcttata
    13261 tgaaatggaa atataccgat caggcatgga tttcttcaat tccggagatt actacctcgg
    13321 ggacatatac cctgcaaccg cttacttcgt caaccaacaa ctgctacaag attccttccc
    13381 cgaattcatc ctctgaattt tatgttgtag agtaccgcaa ggcaacaggc acttttgaag
    13441 ggaacctccc cggttcggga ctgatcgttt accgtatcga tccaactgtt agcggcaatg
    13501 ccgacggccc tcccgacgag gtttatattt accgtccgaa tggtacaacc accaccaacg
    13561 gttcacccaa ttcggcttac tattcttcaa catcaggccg gacggccatc aacgatggca
    13621 ccaatccgtc cccgttttta caggatggca gcgccggtgg attgaattta tataatgtga
    13681 cttcggccgg taccacgata tcatttacgg ttggcatgag taatgtacaa aatccttccg
    13741 catttacagc tactgcagtg agcactaccc aaatagacct ctcatggcag aagaacagtt
    13801 ccaataacaa tgtaatgctt gtttacaata ccacttctgc aatcgggaat ccggttgccg
    13861 gtacttcgta tccggccgga agcgccattc cgggaggcgg caccgtaatc tacaacggaa
    13921 gcggaacaac cttcagtcac acagcgctca cgccttctac aacctatttc tataaactat
    13981 ggtcggttga cggaaccaat aattactcca acggagtttc gaataatgcc acaacgttat
    14041 gcggcattgc taccctcccc tacacggaat catttaccaa ctcaacgttt cctgcatgct
    14101 ggggcaccca cactaccgga agcagcacta ccaacagttg ggaaccttcc ggcaccagca
    14161 atgccggggg taccgcctgt gagatgaaat ccacgtatca ggaggtgaat cccggcgcaa
    14221 cccgtctggt gtccccttcc ttcaatacca cgggaattac ccagttgaac ctgagtttcc
    14281 gtttcctgct cgacgactgg gcccccggag cagtcatgaa ggtgcaatcg agtacggacg
    14341 gagttaactg gaccgacgag gcctggtcac tggcaacctc atcaaatgca gcggtcggac
    14401 cttatctggt aaatacaacg gttacctcta accttaattc cacgactact tatattgcct
    14461 ttactgccga aggcgatctt tatcagtacg attactggta tattgatgat gtgagcatac
    14521 aaaacggcgg atcgcaaagc tacctgatta caacggcttc caatccggtt tcgggcggca
    14581 gcacttccgg cggaggcaca taccagattg gccagaatgt cactgttatt gccacgcctg
    14641 ctgcaaactg gaattttata aactggactg caggcggaac ggttgtatct gctgaaccta
    14701 catacacttt tacggcatct gccaacctcg accttgtcgc caatttcagc atgaaccagg
    14761 tttcggtttc aacaacagcc agcccggctt ccggcggaac gaccaccggc gacggaacat
    14821 acctgattgg agatccggtg actgttacag ccacacctaa tgtcgggtat gaattcgtca
    14881 actggaccga aaacggaaat atcgtttcaa caaatgttgt atatgcattt acagcctctg
    14941 ccagtgtgaa cctggtcgcc aatttctccg tattgcaata tacggtttcg gtaagtgcca
    15001 gtccggttgc cgggggcacg gtatccggcg gagggactta ttcatacaat gcatccgtct
    15061 cggttgttgc ttcagccaat agcggatggg acttcatcgg gtggttcgaa aacggaacca
    15121 tggtatcggg gaatgccgca tactcgttta atattctgga aaaccatgtg ctggaagccc
    15181 actttgttca gcagataccg gtgtatacga ttacggtatc ggcgaatcct cctgaaggcg
    15241 gaaccgtttc gggcagcgga tcggctgcgt ccggatcgca ggtcactgta actgctgcag
    15301 ccagccaggg ctgggtgttc accaactgga gcgagaatgg taatgtggtt tcgaccaata
    15361 gcgaatatac tttcacgata accggcaatg tgtcgctggt cgcaactttc gcttatgttt
    15421 acaatatcac cgcctcggtt tcgcctgcca acaccggtta tatcaccggc gacgggcctt
    15481 atgcctccgg ttcaacggca actttaaatg cctatccctt cctgggatac cagttcgtaa
    15541 actggacact ggacggaaat gtggtttcca caaatccgga atattctttc accgtagtat
    15601 cggacgatca ttttgtggca aacttccaga cagaggttgg cgtttccgat catggcaaac
    15661 agtccttcag ggtttatccg aatcctactt ccggatacct gaccattgaa tcgtcggccg
    15721 gcggatttga aaaaatcacc ctgctcaacc aggttggaaa gagtgtaatg atggtcaatc
    15781 ctgatggctc gggccaaaag atagtcctga atttatccgg attaaatccc gggatttatg
    15841 ccttacaatt gaaattccgg aatggaacag cctccactac caaggttgtg ataaaataag
    15901 aataacattc tgaataaaat gaagatgccg gctgaacgtg aagttcggcc ggcatcttta
    15961 ttattggctt cagtgtgaat ggccggttac agttttttca attccatttc caatatctcc
    16021 agcatcgggt cggtaatatc gagatgggtg acaaaacgga tcacctgggg agacagcgcc
    16081 atggcatgaa tgtctttctg tcttaacttt tccaggaaat catccgaagg cattgagttg
    16141 tcgaggtgaa aaaagatcat gttggtgtcg atagggacta tttcgtttac gaacggcaga
    16201 ttcctgagca tttcaccgat ccggcgggcc ctggcatggt cttctttcag ccgctgaatg
    16261 ttgttcagga gggcatattt tcctgcagcc gccaggaatc ccgcctgacg cattcctcct
    16321 ccgaagactt tacggatacg cctggctttc cggataaatt ccttattgcc gatcagcacc
    16381 gagccaaccg gggcgcccag ccctttggaa aggcagatgg agatggaatc gaataactgt
    16441 ccgaaatcag caggcgtttt tcctgtttct acgatagcgt tgaacaggcg cgccccgtcg
    16501 aggtggtaca tcaggttatt ttcgcggcaa actttttgga tggcgatcag ttcgttgaaa
    16561 tcgtaaacag ccccgcctcc cttgttcatg gtgttttcaa cgcataccag gcgggtcagg
    16621 ggacggtggg gatcctcagt tgggtttata tgtcccttca cctgctcagc cgtgatgcgc
    16681 ccgcggtcgc catccagaag gcataccgaa caacctgaat gaaaggctat accgccgcct
    16741 tcgtaatagt aaatatgcgc cagatggtcg caaatcactt cgtcgccggg ctgggtgtgg
    16801 gctttaatgc caacctgatt ggattgtgtt cccgaggtgc aaaacagcgc agcctccttg
    16861 ccgaatattc cggcaacata attttcaagg tcattcacgg tcgggtcttc accgaaaacg
    16921 tcatcgccga cggctgcatg catcatggca tcaagcattc ccggcgtggg acgggtgacg
    16981 gtatcgctcc tgagatcaat catttctgtg ttgattagta atatttacaa gtattgtttt
    17041 gagaattgca tccggttggc cccggcttaa atttcgcagt caatgcagac tttagcagcc
    17101 gaaaactcct tgttaaacct tataaactcc tggtgggcag ggtgatcggt atacgtgtta
    17161 aggtcgttca gattgtcgaa atccatgatc aggacgatgt cgtacgacat ttccagtttc
    17221 cggatatcta cgccgacttc gcaacggcgg atcaggtcaa ttttaccggg aagttcttct
    17281 atccggttct ttatttttaa ccggatttcc tgtttacgga tttcactttc aaaatccctg
    17341 agcttaaaaa ggacgatgtg tcggatcatg gcgtatgagg attaaataaa cgcttctttc
    17401 atctggttca atgctttccg taaaacctcc cgggtgcagc cgatattcat ccgcataaat
    17461 ccttcgccgc cgggcccgaa ttgtattccc tggttgagcg cgagatgcgc tttattcagg
    17521 aagaaatcct tgagctcaac atccgtcatt cccagttcgc ggcaatcgag ccacatcagg
    17581 aatgttgctt ccggcctgac gggctttatc tgcggtatgt tttcggaaag gaacgattcc
    17641 accagcgata cattgccggc aaggtaatcc atcagggcat ccacatattt gtcgccatgc
    17701 gtgaaggccg cttccatggc tacattcccg aagatgttgc caagcccgac atgaaggctt
    17761 tcgagggtgg cgacgaactg ttcccttagt tttttatcag gaatgatggc tgccgaagag
    17821 gataagcccg atacgttgaa tgttttactc ggtgcaataa aggtgacggt gttagcggct
    17881 atttcgtccg acaggcttgc catcggggta tgtacatggg gcttgtatac caggtcgctg
    17941 tggatttcgt cggaaacgac gaggatgtta tgcctgaggc atatttcggc aatcctgctc
    18001 agttcatcac gggtccatac atttccgccc ggattatggg ggcttgaaag gatgatcatt
    18061 cgtgcgccct ggcggcattt ctgttccaga tcggtgaagt cgatgctcaa tctcccgttg
    18121 acaagtttca gcggactata aatcagtttg cgccggtgat cggttgcagc gttaaaaaat
    18181 gggaaatata ccggaggctg ggtgataatc ccatccgaaa cagaggtata cgccagcacc
    18241 gatatgttga cagccgggac aataccgggt gtgaaaacga tccagtcggt tgagatatcc
    18301 cagttgtgcc ggcgctttac ccatccttta atggcgtcga ggaaatgctg ggggcggagg
    18361 ctgtaaccca tcacttcatg ttgaagcctt ttcttcaatg cgtcgatgat gaattgaggc
    18421 gttttgaaat ccatatcggc gacccacatc ggcaacaaat cagcagaacc gaaaaactgg
    18481 gtgagcccgt catatttcag gctgtcggta tgaagcctgt caacgggctc atcaaaatta
    18541 aaagtcataa ggcagttcat tttcaaaggg ccaaagttac cgaataacag gatttatttg
    18601 tgctttcgtg caaacagtta tttgtataac tttgctgtta accgctgtcc ggataactgc
    18661 tgagaagatg aaaccctcgc aattggcgtt gctttcgcaa aagtattatt ttgatgatac
    18721 gccctttatc aacctgatga agaagcgcat ctatcatgta ttgcttattg ccagcaatta
    18781 tgatgctttc atcctggaag gcgacgggcg catcgacgaa cagatcttta atgagtatgt
    18841 gtcgctgaac ctgcggtacc cgccgcagtt tatacaggtg ccgtcggaaa aggaggcgtt
    18901 taaaacgctc agggaagaga aaatagacct ggttatctgc atgttgaacc ttgaagagct
    18961 ggacaccttc gagctggcga gaaagataaa atcggagcat ccgaaaattc cgatcgttgt
    19021 tcttaccccg ttttcacgtg aagtgtcgct gaaagttaca catgaagacc tgagcgctat
    19081 cgattatgta tttagctggc tgggcaacag cgacctcctg ctggcaatca tcaaactgat
    19141 tgaggataag atgaatgccg aacacgacac cagtgaagta ggggtgcagg tggttctgct
    19201 tgttgaggac tccatcaggt tttattccag ttaccttccg aatatctata agatagtctt
    19261 taaccagtcg cggtcgttca tgaccgaggg gctgaacgag caccagcaga tgctccgcaa
    19321 ccggggacgg cccaaaatac tgcttgctac caattatgaa gaagcgatcg acctttatga
    19381 aaaataccgg aataacctgt tggggattat ctccgatatc aattaccggc gcaagggcaa
    19441 ggaagatccc gaagccgggt tcaggcttct gaggcatgtc cgcggcaagg acgagtttgt
    19501 tccttttttg ctgcagtcat ccgacgaaaa atatgcctct gtcgcccgcg agatgaatgt
    19561 cgggttcatc aacaagaact cgaaaacgct gtctatcgag ttgcgccact tcatgatgga
    19621 atatttcgct tttggggatt ttgtgttcct gaatccggaa acacatgagg aaattatgcg
    19681 tgcaacagat ctgaaagcct tgcaacagcg catttttgag gttcccgacg aatcgctgct
    19741 ctatcatatc taccgcaacc atctctcgaa atggcttttt gcacgggcgc tgtttcctct
    19801 tgccgaattt tttaaagatg tgcgtgcagg tgattttacc gacctggatg aaatccgcag
    19861 gttccttttc gaagccattg ccggttatcg gctgaacaag gcgcggggcg ttattgccga
    19921 gttcaaccgc gacagtttcg atgaatatgt cacctttacc cgtatcggcg acggttcgat
    19981 cggaggtaag gcccgcgggc tggctttcct cgataccatc atcaaacgga acaggctttt
    20041 cgataagttc ccgggggtaa atatttcaat tccgcacacg gtggtgctgt gcaccgatgt
    20101 gttcgatgaa ttcatggaag ataatgatct atacaagatc ggcctttccg atattccgga
    20161 tgaggaaatc ctgaatcagt ttattaatgc gaggctgcca tttcgtttac acgacgatct
    20221 ggggaaattc atcagcgtca gccgccgccc catagccgtc aggtcgtcga gcctgctcga
    20281 agattctcat taccagcctt ttgccggaat atattccacc tatatggtgc cacaggtctc
    20341 cggtgatgac cgcctgatgc tcgaacaact cagcaatgcc atcaagagcg tttacgcttc
    20401 ggtattttac cataacagca aggcttacat ggccgcaacc cgtaacctga tcgacgagga
    20461 aaaaatggcc atcgtgctgc aacaggttgt ggggtcccat tttggcggaa cattcttccc
    20521 cactttttcg ggcgtcgcca ggtccattaa ttattacccc ttagcgcccg aaaaaagcga
    20581 agacggcatt gccagcatcg ccatcggact gggaaaatat atcgttgacg gaggaacatc
    20641 cctgcggttc tcgccgcggt atcccacgaa agttctgcaa ctctcaagcc ctgaaatggc
    20701 tgtacgcgaa acgcaaaagc atttttacgc cctcgacctg aagccgggca gcttctggcc
    20761 atccctggac gacggcatga acatgaagca cctgaaaatc gaagatgcat cggaagatat
    20821 ggcttttaaa gacatggttt ctacttttga ttttgataat aatgtgcttc gcgaaggagt
    20881 caatattgaa ggaaggcggg tggtaacctt tgccaatatc ctgaaccata attcgttccc
    20941 gttggctgaa attctgcgga tatgccttga aatgggacac cgggaattgg gaaaaccgat
    21001 cgaaatagag tttgccgtgg atctgcacag gcccaagtcc accccgaaag cctttacact
    21061 gttacagata aggcctattg tggaaagcaa ggagtcgaac gatgtgaaga tcggcgatgt
    21121 gaaaacagaa aatgccctga tctactccaa tgctgcactg ggcaacggcc tgataaccgg
    21181 aattgaagat ttggtgtatg tgaaacccga ggcttttgac ccggcgcaaa attcaggaac
    21241 ggcaacccgt atcggggaac tgaatgataa gttcctggct tcgcagaaga actatatact
    21301 ggtcgggccg ggacgctggg gcagcaccga tccgtggctc ggcatccccg ttaaatggcc
    21361 gcagatatcg gcagccaggc ttatcgttga atcagggctg gaaaactatc gcattgatcc
    21421 cagccaggga actcattttt ttcagaacct cacctcattc agggtcggat attttaccat
    21481 caaccccttt atgagcgatg gctttatcga cctggagttg atgaattcat tgtcagccat
    21541 tttcgaagac gatattatca ggcatgtccg gtttaaccag cccctgacaa ttaaaattga
    21601 cgggcggcac aacaaaggcg tcgttctcaa accttcgccg cagacggaag atgaccagga
    21661 cgaaaataat tagcgcaggc aggcgttcat tcttcaatca tttcttccgg atgaagatgc
    21721 ccgggatgaa cttgaatggc ctgtatcaat tggaattgta tttcagaacg ataaataaaa
    21781 cccgatgaaa aaattaaacc agtggactat cctcctttta ttaccggtgg tcatacttgc
    21841 cggttgcaag aataaaaaag ccgatgaaaa atctttgatc acgcgacgga tacaatacga
    21901 tgtgcccatc aaagcctccg atgccgacaa cgactggtgg gtgcagaaca tcgaagggca
    21961 ggaccgggaa aaactggtga aggagatcat ggaatcggcc cttagcggca aggtgaagac
    22021 ctatgatttt ttttccaacg aattgctcac acctgatcaa ctcaaggcca tcatgaagcg
    22081 ttcggatacc attaccctgg aacgtgccgc ccctccgcac agcctttacg atacagtcct
    22141 cacgaaagag cttagcctgc gcgatatcac acgcctgcgt tttctggagg agtggaatat
    22201 ggacaaaaaa acacttcagt ttaacaagaa agttgccggc atttgtccgc ttattgccaa
    22261 ctacggcgac agtgcagaat tgcgcggtta tatgccgctg ttctgggtgt tttttgatga
    22321 taaatatccg ggtgaactga aaatgaaata gtcctgccgc cagaggcggg ggcagggagc
    22381 agggggcagg tagcagaacg gagaacgcaa accactaatt ccccgaatgt caatcacgaa
    22441 ttccacgaat cactaattat ccga
//
LOCUS       DAOPYV010000051        21871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_2429_length_21871_cov_12.771223, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000051 DAOPYV010000000
VERSION     DAOPYV010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21871
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2429_length_21871_cov_12.771223"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..6425)
                     /locus_tag="PKI35_07840"
     CDS             complement(<1..6425)
                     /locus_tag="PKI35_07840"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNX44328.1"
                     /translation="MKTNLRNMRKLFFMLFLVAFAAWNSYGQETTYYAFAQSNGTYNE
                     ITGTDILGNPFDDQTAVVSLGFTHLGNGWSKDQIRVSANGWLYFPNTSTSSLGYTPIS
                     SSITGIYLVVAPFGRDLRSIDATSSVSYLLSGVAPNRVFTVQWKNVKRYGSAYAGEIL
                     NLQVKIYETTSKIEMIYGPCTAITGTNYPQVGLRGLTNTDFSNRTSTTGWNNTVAGTV
                     NTETVTTNATYPPINGLTFTYTYTFLPPAAATIVSPANGETDVPRQTTLNWGNGGSGG
                     GPFTGYTLYLGTDNPPTNLVNGLDMGSATTYDPVPDLLATTTYYWRVDSYGPGGNTTG
                     TVWSFTTLTPTIISGWVRNSALNPIIGASVYAGAYNTMTGAGGAYELELIPGTYDMIV
                     TAPGYNSATATGIVVPPASNVTQNFTLTNPTMSITPNPFNVTVNPNELYTENMYILNE
                     GTGQLDWSATIAFPPGDNAMGGGIQPPLLSPGTGGVETTRAGFANDPVDPNNSDAAGD
                     ILSSFVIPNPPISLGWGLGVEGNNLWMSSYNDKKLYKFSPSGSIITSFDYSSWAGANW
                     VGDLAPGPAGYIYAVRVGTDNAIHKINTGTGADEGMITGAWATTSARGVAYDAVHNEL
                     WVGGWNHSQIYHVSATGATISTISFSGVAGLGWHKAGNGGTGSLWVVTNASPSVVYEL
                     NPATGAILKSFVVPGVSGYTGAGLEIDAQGNVWLINQTNQTVYKIDSGTPIVSWLTLE
                     EYSGTVPPMTNYALPAHFNAFGLLAGTVKTATITFTSSPDVGTWNIPVTMTVQGTPLE
                     TPQNLAATLVNQLTGEVSLTWDAVDPTGLLYYAINRDGVQLTTTSNNYYTDMLPTYGT
                     YNYTVQAVYSAGLGVPSNPAEVEWPNPTLTLDPASLTATVWTGTDKDVPFTVGNIGEG
                     TLAYEFPDYTDDDAPLAYCAASASYCDEYISRVQFNTIDNSTGCSYYADYTSISTDVK
                     AGDTYPITLTNPSPWSSDQAALWIDYNHNEVFDNDENYPLTQGGGGATFTGNILIKET
                     SLPGPTRMRIRLSYAATPAPCGTQTYGEVEDYTVNVIQNFITQVIPAQGTVAGGDDTE
                     VVATFSATGSFAPAGTYTQSLLLTTNDLAHPSVTIPCTMIVTIPGTISGTVTDCITGE
                     PIQGVMVAADPFTVMTDENGNYSLVCDENTYNVVFSKVGYQSNTATGVVVTSGATTTV
                     NAQLCEMPYAPGCANAVVNATDTQCDVTWCVPMGPYELSYDDGTAENFAAWQLAGNLN
                     AVKFTPAGYPATVIGGMFYVGDGTFPAGGNIINSTFTVRVFDDDGATGLPGTQIGDSV
                     LATVTATGWVTVTGLNATVTAGSFYLAMEQNEAEPNCAPIGVDETLPKAYKSYSKFVT
                     AGGEWALSAYQDFMIHAIVDGPVADDDAVASTQVLVPAKASLAGVISQRAPYTLPGVE
                     GKAVITSPEGYVPDAITNYILYRYPVTDPSIPVYGTEALLADDLTTNSYVDGGSVWSA
                     LPQGWYAYGVKAVYPNGQESPITYTNIVGHKMTADVTINVQLVCGFVPAVGAEVQMIG
                     LDYPYQTFSATTPASGSVTFNVWKGNYTVDVRTTGYLPYVVNYNITGPTPIDVILEDA
                     KYAPRNLYVDANTLVATWEPPLLALVDENFEGATFPPSGWSETGNATVGWFATTAGGS
                     SYFPVPSHTKYAIVNDDDNDEDNCCTYLITPNLDLSTAPGFVMNFDSYFTGAWGGTAT
                     VELTTDGGATWTTIYTVPAAGSWGTKTIDLSAYSGPGGLTSAKIGFHYNDNGSWADGW
                     AIDNVNVTVGMTPTYGYGVFLDGSLAGNTNELTWTYNPSAMLWGHTYVAGVAGLYCSG
                     YSDMATYTFTSNFLFPPRNLQGLANDNAAILTWEPPVSGDGMDNAGTKPDYSWMQSNG
                     GTLSTTGNPGNAAPGDGFTAKANRAETEIYYCGEKDDAIGTGGAVEFMVAARFTNDEL
                     YQYYGLKQITKVSMFCSNSGAWSNVTVKIWEGGSLGDPGTEVCSEDVTSQIISEDWTT
                     VTLTTPVPLVAGNEYWIGYYINATSGWPAGCDAGPAVDGKGDWMYYDGAWQEIGPAFG
                     LDYNWCIKGIIDDAGPQPSQNLVSYVLYRDNAAIAEVDKTQLEYWDLNLNPATYCYGV
                     TAKYDMTDYG"
     gene            complement(7055..10057)
                     /locus_tag="PKI35_07845"
     CDS             complement(7055..10057)
                     /locus_tag="PKI35_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNX44329.1"
                     /translation="MVLRFTKAMRNGMNKAGWNLMIALCLVTVSLSGNAQIYVGDTLT
                     ENTTWSKTGQDYIVVQSVIVPKGITLTIEPGVKVLFEVNTALEVRGTLIAQGTPSDSI
                     MFLPEDDLWNGIYFHGALTEFDDLGNYTGGTILSYARFENSAYSVVLQENTGILIEHC
                     CLKRASFGIYMKDALNNVIRNCRIGDSDFGIFMASSYVAQYNLFLNNAVTNNRYVGFF
                     INNSSGQISHNTISGNNVSGNLVGLYLGNDGPVDEGYNQVSENIFNNNSFGIRLFQDT
                     SVIERNYIVNSLINGIEIIGACRSTVRNNLISGNNLWGIVVEQGASDNLIKTNSVEHN
                     QNGILFTPGKTGSSLFNSFVANSVHHNADTAFSFMSVPQGLFVSNSIYENRDSAAFLN
                     HTAQLIEAVGNWWGTSDTNTINTFISDVFDDPAKGLVNYQPFHNGPDTEAPVKAPAYA
                     VKKDVGNGIQVSWPPNTESDLAGYRIHYGTFDGFNYQYSLEAGTDTSIFLQGVSLDDT
                     IAVTAVDTEFDGLFDRPEGHESDFTIAIPGPWAGPNSEVCENGELLLSEATANGYNLL
                     SWTTTGDGTFDNPRILHPVYTPGQGDVAIGAVYLTLHISGEGFNLDDEMRVKVRPAPY
                     VFAGSDTTIISGSHLLLSSAAADYFTGLHWLTLGDGTFSNDSVKNPDYFPGAGDLASG
                     HVNLILDISSPCGPASDTIKVQIVEGYFIKGRVHAGGNLVPGSTLNLYRCSGNTVSAM
                     RSTLTAMDGTFIFDLLIEGEYYIYAIPPVNLADQYAPTYYFNDLNWENAYRFNTEANT
                     YDVDIELFKLPHQIPEGPGSISGSCETGGPFLCGDVTILLYDKTLKYLLGWQRLDDEG
                     NFNFRNLPFGDYVLVGEKAGYQKNETPLISLTPEQPAISGILVKILPFKIGIAMPEGY
                     DPSLENIVVAPNPTHDIVSISGLADGENYRIILRDTYSRLLGITGRSQGTGSLPLDLS
                     LRALPAGLYFIEIQRDNGIVTRYKIIKE"
     gene            complement(10147..11472)
                     /locus_tag="PKI35_07850"
     CDS             complement(10147..11472)
                     /locus_tag="PKI35_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44330.1"
                     /translation="MYRSFIIISFLLITVASADAQLSVLNRPVTVTAVNQPLSEVLNK
                     IGRQASIKFSYDPDELSARRLVSIQAKREPLNKVLVQLLNNPALSFRAMGNQVIIFKP
                     AAEADNAAEKPKPEAQRYLNKNSAIRLKPDTISHATRFRIDTVFITNTETILRIDTLI
                     FRDTVVHRDTVFIEKYKTRKIKGNRNSNFDKELVKNRKFQENNGFSVGLSYSQYFGKA
                     TYAGGQGSLSELLEKSRQSESVSLQNFSAGISITYDYKRIGAGTGLFYTRLGEILDHE
                     YVEQTGGYYLRDTVETYYTVEGTDTLRYYITDSTWQNLNSTKFSYTNPNAFRYLDVPL
                     SLRLFVIKNENFNLYMKGGAVAGILIGSKALVVSPEDYKAEWIDRNRLNPFVFSWIAG
                     LGAGFRISGKLSLEAEAFYRRQVSSLYSDYPLDKTYRLPGFRAGMCIKL"
     gene            complement(11521..12495)
                     /locus_tag="PKI35_07855"
     CDS             complement(11521..12495)
                     /locus_tag="PKI35_07855"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HNX44331.1"
                     /translation="MRFLANECSGEEQRIISELINTDKRYRSIYRHLQRTWHSTRPSA
                     GEETYDVDEAWAKVQNRIDAKQEPVIRTLHPHPIRKIVYAVSGAAAILLIGLGIFQYV
                     RPSDDLNTFASGSAIAVPVNLADGSVIHLNKNSEIKFPEKFGHDNREVYFWGEAFFSI
                     ASDKTRPFIINAGETRIKVLGTSFNVKDYEETGKVEVTVKTGSVLFYYVDKDENALGQ
                     VTLSVGDKGIYDRKAGRIFKTVNTDLNFMSWKTGILVFDEATLGDVFDAIGNRYGVSF
                     DIKNKQIKNLRLTATFDKDQSLDSVLEVIRLVHSVEFVKTGKDYLVTK"
     gene            complement(12611..13189)
                     /locus_tag="PKI35_07860"
     CDS             complement(12611..13189)
                     /locus_tag="PKI35_07860"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HNX44332.1"
                     /translation="MEESEKILIDKLKQGDRPTFEHIFRTYYVPMCSYANRYLSDKSA
                     SEEVVQDLFCKIWFRRSELVINTSLKNYLLRATINHALNTVKRKEVDRKYVDYIGFKV
                     EEAYSDTDDLVQQELQRRISLGISSLPERRREIFEMSRFEGLRYEEIADRMKIKVKTV
                     ESQMVKALEFLRKYLHEYVTPIVLIISMIIKN"
     gene            complement(13288..13605)
                     /locus_tag="PKI35_07865"
     CDS             complement(13288..13605)
                     /locus_tag="PKI35_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44333.1"
                     /translation="MFLWFISNIRLYCDGKFIENANVMKANIRFSGDIGGLWQTYAET
                     TRLEPAKPIALQGRILPSGTLSRWRNLEWHSCFQDWSHQVQGDQRSGAGARRDTICPG
                     HNS"
     gene            13770..14081
                     /gene="rplU"
                     /locus_tag="PKI35_07870"
     CDS             13770..14081
                     /gene="rplU"
                     /locus_tag="PKI35_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNX44334.1"
                     /translation="MYAIVDIAGQQFKVKKDQKIFVYRLQGEEGASVEFKDVLLLDNE
                     GSISIGTPHVEGALVTARILTHTRGEKVTIFKKKRRKGYQKESGHRQDLTQLQIEAIT
                     A"
     gene            14132..14395
                     /gene="rpmA"
                     /locus_tag="PKI35_07875"
     CDS             14132..14395
                     /gene="rpmA"
                     /locus_tag="PKI35_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNX44335.1"
                     /translation="MAHKKGAGSSQNGRESHSKRLGVKIYGGQFAQAGNIIVRQRGTV
                     HYPGINVGLGKDHTLFALRDGVVEFKKRRNDRSYVSVHPLDAE"
     gene            14563..15834
                     /gene="serS"
                     /locus_tag="PKI35_07880"
     CDS             14563..15834
                     /gene="serS"
                     /locus_tag="PKI35_07880"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005469625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNX44336.1"
                     /translation="MLELSLLRENPQLVIDRLMVKHFDAKDIVAKILDADQSRRETQK
                     LLDDNLAEANNLAREIGVLFKNGRQQEANDLKTKTAELKATAKNLSEKLDTLQKEQDR
                     LLVILPNLPHPDVIPGNSSEDNVEVHREGDIPVLPDNAMPHWDLCQKYDIIDFELGVK
                     LTGAGFPVYKGKGARLQRALINFFLDNAIRAGFHEIQPPLMVNEASGYGTGQLPDKDD
                     QMYFIGLDKFYMIPTAEVPVTNIYRDVILNATDFPVKNVAYSACFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQIQHPDKSYETLEEMREYVAGLARTLELPFRIVRLCGADMSFTSA
                     MTYDFEVFSAAQKRWLEVSSVSNFESFQANRMKLRFKEGSGKTRLAHTLNGSALALPR
                     IVAALLENNQTPEGIRIPKALVPYTGFDMIP"
     gene            15956..17791
                     /locus_tag="PKI35_07885"
     CDS             15956..17791
                     /locus_tag="PKI35_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX44337.1"
                     /translation="MKTISCNRLLLSALFMVLLRPVMAQQQTDERLGMEYYSTRQYDK
                     AIEVFSRLYEKSPTLFSYIYYVNCLFEVKDYDKAEKLIRKQMRANPNDPRYTVDLGYL
                     MAIQDETQKGKKLYDECLRNLKDDRNSVYNTAAAFLNRRETDYAIRTYLKGRDLLKDK
                     SAFAIELAIQYESQGNSAAMLDEYLTLLADKPEQMATVQNRMQAWLSDDPDNTKNDLF
                     RSTLLKKSQQNPDEVSFAELLLWYSVQQKDFKLALLQAKALDRRYGEDGQRVYDLARL
                     SFTNDDFDVTTDAYAYIIKKNADADLVLKSRIELLNTELAKAIQLHSYDDKFLKALEA
                     KYLSLLKESGKNPATIPLIRNLSHLQAFYLNNTDSATALLEDAIIIPGITDRLQAECK
                     LELADIYLFSGEPWEATLLYSQVEKAFKNEPIGHEAKFRNAKLSFYICEFEWAKSQLD
                     ILKAATSKLIANDAMSLSLLISENMDDDSTYGALSMYARADLLLFRNRDQEAMDALDS
                     LLIRYPHHSLADEVLFKKAEICMKEKKFEDAVKYYREIVADFPTDLLADDALYALAGI
                     YDYITPDKQKAMELYQTLLTTYPGSLFVVDARKRFRILRNDPVNL"
     gene            17815..18579
                     /gene="rsmA"
                     /locus_tag="PKI35_07890"
     CDS             17815..18579
                     /gene="rsmA"
                     /locus_tag="PKI35_07890"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNX44338.1"
                     /translation="MEPVRAKKHLGQHFLKDENIARKIAESLSPGVSQVLEVGPGTGV
                     LTKYLIANHYSSFKAIELDRESAAYLKSAFTLGEDLIHADFLRTDISALFEGPFAVIG
                     NFPYNISSQIFFKVVENRDKVSECVGMIQREVAQRMVSKPGTKDYGILSILLRAWYDI
                     EILFIVPPQVFVPPPKVTSAVVKLTRNGIVDLGCDTKLFSTVVKTAFNQRRKTLHNSL
                     KPLADYNGEFTGKRAEQLSISDFVTLTNDIAETCSQ"
     gene            18677..19777
                     /gene="meaB"
                     /locus_tag="PKI35_07895"
     CDS             18677..19777
                     /gene="meaB"
                     /locus_tag="PKI35_07895"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNX44339.1"
                     /translation="MNSSKDKPFRSALKVNEGIEQPPVVNELAASRFRKARHNLLPAG
                     EYLDGILSGNRTILSKAITLIESSLPQHQELAQEIIAHCLPLSGNSVRLGITGVPGVG
                     KSTFIEAFGLYLISLGHKPAILAIDPSSSRSKGSILGDKTRMEELSANENAFIRPSPS
                     AGSLGGVARKTRETIILCEAAGFDTIMVETVGVGQSETTVHSMVDFFLLLMLAGAGDE
                     LQGIKRGIMEMADAIFINKADGDNLQKVTIALAEVRNALHFFPPAESGWQTVAGTCSA
                     VTRSGIPAVWKAIEDYLSLTRKNGYFHHRRLEQARFTMHQSVEQQLNDRFYHDPEIKK
                     LLHSLEHDLSTGKLSAYMAAQKLLDAWDRKNK"
     gene            19908..21425
                     /locus_tag="PKI35_07900"
     CDS             19908..21425
                     /locus_tag="PKI35_07900"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNX44340.1"
                     /translation="MKNPFFNSLTAMNPNSLVQYLNKPSDDFTKSDIIRYIDDHNISM
                     INFRYAGGDGRLKTLNFVINSQEHLDSILSTGERVDGSSLFPFVKAGSSDLYVVPRYK
                     TAFLNPFAQVPTLDILCSYYDKDGNPLESSPEYILRKAHNVLKDRTGLTFHVMGELEY
                     YVISQKNELFQAADQKGYHESHPFAKWEALRAEAMVAIAQTGGQIKYGHSEVGNFSLN
                     GLEFEQNEIEFLPVNLEDAADQLVIAKWILRSLAYKYGVSLTFAPKITVGKAGSGMHI
                     HTRLMKDSRNAMVEDGQLSDTARRAIAGYLDLAPSLTAFGNTNPTSYFRLVPHQEAPT
                     NICWGDRNRSVLVRVPLGWSGANNMINHANPQEEKDEKDYSDKQTVEFRCPDGSADVY
                     LLLAGLTVAARHGLEMKDALEYARKTYVDVNIFDEAHKARVAELKQLPVSCWDSAEML
                     LKQADIFKMYDVFPQSVIEGTANKLKSFEDRGLRESLEGNTEKLQEIVNRYFHCG"
     gene            complement(21582..>21871)
                     /locus_tag="PKI35_07905"
     CDS             complement(21582..>21871)
                     /locus_tag="PKI35_07905"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44341.1"
                     /translation="DSDIAGKNRGFLNQNTPNPFSESTQINYSIARAGNVDISVRNLT
                     GQEILSLPQGTQGKGSYSVGFFNQTLPSGFYFFTLRIDGSDVATRKMCVIR"
BASE COUNT         5741 a         5394 c         5219 g         5517 t
ORIGIN      
        1 ccgtagtcgg tcatgtcgta cttggcagtt acgccatagc aataggtagc aggattgaga
       61 ttgaggtccc agtattcaag ttgggtcttg tcgacttcgg caatcgcagc attgtcacgg
      121 tacagcacat agctgacaag attttgtgaa ggctgagggc cggcatcatc gatgatacct
      181 ttgatgcacc agttataatc caatccgaaa gcgggtccta tttcctgcca tgcaccatca
      241 taatacatcc agtcgccttt accgtcaaca gccggaccgg catcgcatcc tgcgggccat
      301 ccgctggtag cattaatgta atagccgatc cagtattcat taccggcaac gagaggaacc
      361 ggagttgtaa gtgtcacggt ggtccaatct tcggaaatga tttggcttgt aacgtcctct
      421 gagcatactt cagttccggg atcgcctaaa gagcctcctt cccagatttt taccgttaca
      481 ttgctccagg caccgctgtt tgagcagaac atgctgactt tggtaatctg tttcaaacca
      541 tagtactgat agagttcatc gttggtgaaa cgtgcagcaa ccatgaattc aacggcgcca
      601 ccggttccga tagcatcatc cttttcaccg cagtaataga tctcggtttc ggcacggttg
      661 gcctttgcag taaatccatc accgggagct gcatttccgg gattacctgt tgtcgacaag
      721 gttccgccat tgctctgcat ccagctataa tcaggtttgg ttcctgcatt atccattccg
      781 tcgccactca ccgggggctc ccaggtaagg atagccgcgt tgtcgttagc cagaccctgc
      841 aggttccgcg gcgggaagag gaagttgctg gtgaaggtat aggttgccat gtcgctgtaa
      901 cccgagcaat acaaccccgc aactccggca acataggtgt gtccccacaa catggccgaa
      961 ggattgtagg tccaggtcag ttcattggtg tttccggcca gcgatccatc caggaatact
     1021 ccgtatccgt aagttggagt cataccgaca gtaacattta cattgtcgat agcccagccg
     1081 tcagcccagc ttccgttatc gttataatgg aaaccgatct tggccgaggt aagtccgccg
     1141 ggaccagaat aggccgacag gtcaatggtc ttggttcccc acgaccctgc tgccgggacg
     1201 gtgtaaatgg tcgtccaggt agcgccgccg tcagtggtga gttctaccgt tgcggttccg
     1261 ccccatgcgc ctgtaaagta gctgtcgaaa ttcataacga atcccggagc tgttgaaagg
     1321 tcaaggtttg gagtgatcag gtaagtgcag cagttgtctt catcattgtc gtcgtcgtta
     1381 acaattgcat attttgtatg cgacggtacg gggaagtaag agctgccgcc tgcggttgtt
     1441 gcaaaccacc caactgtagc attgccggtt tctgaccatc cgctcggcgg gaaagtggct
     1501 ccttcaaaat tttcgtcaac aagggcgagt aaaggaggtt cccaggtagc aacgagggtg
     1561 ttggcatcaa catacaggtt gcgcggggca tatttggcgt cttcaaggat tacgtcaatc
     1621 ggggtcgggc cggtaatgtt gtaattaaca acatagggca gatatccggt cgtgcggacg
     1681 tcaaccgtgt agttgccttt ccatacattg aaggtaacgg atcctgaagc aggagtcgtt
     1741 gccgagaagg tctggtaagg ataatcgagg ccgatcatct gtacttcggc gcctactgcc
     1801 ggaacaaatc cgcatacaag ctgtacgttg atggttacat cggcggtcat cttatggcca
     1861 acaatgttgg tataagtgat cggcgattcc tgaccgttag gataaacggc ttttacgcca
     1921 taagcatacc atccctgggg aagagccgac catacgctgc cgccatctac ataactattg
     1981 gttgtaagat catcggcaag cagggcttcg gtgccataga caggaatgct gggatcggta
     2041 acaggatagc gatagaggat gtaattcgta attgcatctg gaacatagcc ttcaggcgaa
     2101 gttatgacag ctttgccttc aacgccggga agtgtatacg gagcgcgctg cgaaattacg
     2161 ccggccagtg atgccttagc aggtacaagt acctgggtgg aagcaactgc atcgtcatcg
     2221 gcaacagggc cgtcaacaat agcatggatc atgaaatcct ggtatgccga aagggcccat
     2281 tcgccaccgg cggtcacgaa tttcgaatag ctcttgtaag ccttcggaag ggtttcgtca
     2341 acacctatcg gggcgcagtt aggttctgct tcgttctgtt ccatagccag gtagaaactt
     2401 ccggcagtaa ctgtagcatt gagtccggta acggtaaccc aacccgtagc agttacggta
     2461 gcaagcacgc tatcgccgat ctgggtgccg ggaagtccgg ttgcaccgtc gtcatcaaat
     2521 acccttacag tgaaggttga gttgatgata ttgccgccgg cggggaaggt accatcgcca
     2581 acatagaaca taccgccaat tactgttgcg ggatatccgg caggggtgaa cttaacggca
     2641 ttcaggttgc cggcaagctg ccatgcagca aagttttcag cggtgccgtc atcatacgac
     2701 aattcatagg gtcccatagg aacgcaccat gtaacgtcgc actgtgtatc ggtagcattt
     2761 actactgcat tggcgcatcc gggggcataa ggcatttcgc ataactgggc gttgaccgta
     2821 gtagtcgcac ccgaagtcac aacaacgcct gttgccgtat tcgactgata gccgactttg
     2881 ctgaagacaa cattataggt gttttcatcg caaaccaggg agtagttccc gttttcatcg
     2941 gtcatcaccg tgaacggatc agccgctacc atgacaccct ggatcggttc gcctgttatg
     3001 cagtcggtaa ccgtacccga aatagttccg ggaatggtaa cgatcatggt gcagggaatg
     3061 gttaccgaag gatgggccag gtcgttggtg gtgagcagca acgactgggt atacgtaccg
     3121 gcaggagcaa acgaaccggt tgccgagaat gttgctacaa cctccgtatc atcgccgccg
     3181 gctaccgtgc cctgggcagg aataacctgg gtgatgaaat tctggataac attaaccgta
     3241 tagtcttcaa cctctccgta tgtctgagtt ccgcaagggg ccggagtagc ggcataggaa
     3301 agacggatac gcatacgggt aggccctggc agcgaagttt ctttgataag gatattgccg
     3361 gtaaatgtag cgccacctcc gccttgtgtc agaggatagt tttcatcatt atcaaatact
     3421 tcgttgtggt tgtagtcaat ccacagggcg gcctgatccg aactccatgg cgaaggattg
     3481 gtcagggtga taggataggt atcgccggct ttcacatcgg tggagatgga agtataatcg
     3541 gcataataac tgcagcccgt ggaattgtca atggtattaa actggacacg gcttatatac
     3601 tcatcgcaat aggaagcgga agcagcacaa taagccagcg gggcgtcgtc gtcggtataa
     3661 tcggggaatt cataagcgag tgttccttca ccgatattgc cgacagtgaa cggcacatcc
     3721 ttatcggttc ctgtccaaac agtagctgta agcgaagcag gatccagggt aagggtcggg
     3781 ttgggccatt caacttcggc cgggttcgaa ggaactccga gccctgcgct gtaaacagcc
     3841 tgaaccgtat agttgtatgt gccgtaggtg ggcagcatat ccgtataata gttgttactg
     3901 gttgtcgtaa gctgaacgcc gtcccggtta atcgcataat aaagcaagcc ggttgggtct
     3961 acagcatccc aggtgagtga aacttcaccg gtaagctggt taaccagggt agctgcaagg
     4021 ttctgcggcg tttccagggg agtgccctga acggtcatgg taacgggaat attccaggta
     4081 cctacatccg gtgaggaagt aaaggttatg gtagccgttt ttaccgtacc ggcaagcaga
     4141 ccaaaagcat tgaaatgggc cggcaaagca tagttggtca tcggaggtac cgttcccgaa
     4201 tactcttcga gggtaagcca gcttacgata ggagtccccg aatcaatctt ataaacagtc
     4261 tggttggtct ggttgatgag ccaaacatta ccctgggcat ctatctccag tcctgctccc
     4321 gtgtaacctg acacgccggg tacaacaaat gatttgagga tagcgcctgt agccggattg
     4381 agttcataaa ccacactggg agaagcattg gtaaccaccc agagtgaacc ggttccgcca
     4441 ttgccggctt tatgccagcc aagtcccgcc actcctgaga atgaaatagt tgagatagta
     4501 gctccggttg cacttacatg atagatctgg ctatggttcc agccgcctac ccaaagttca
     4561 ttatgaacgg catcgtaagc aacgccgcgg gcgctggtgg ttgcccatgc gccggtaatc
     4621 attccttcat cggctccggt acccgtattg attttatgga tagcgttgtc ggtacctacg
     4681 cggacggcat agatatatcc ggcaggaccg ggagccaggt cgccgaccca gtttgcgccg
     4741 gcccacgacg aataatcaaa ggaggtaatg attgaaccgc tgggcgagaa cttgtacagt
     4801 tttttgtcat tgtaactcga catccacaga ttatttcctt caacaccgag gccccatcca
     4861 agtgatatgg gaggattagg aatgacaaaa ctcgagagga tatcgccggc agcgtccgaa
     4921 ttattaggat caacgggatc gttggcaaat ccggcgcgtg tggtttcaac gccgccagtt
     4981 cccggcgata aaagcggggg ctgtattcca ccgcccatag cattatcgcc cggagggaaa
     5041 gcaatggttg ccgaccaatc gagctgcccg gttccttcat tgaggatgta catgttttcg
     5101 gtgtacagct cattggggtt aactgttaca ttaaacgggt tgggcgtaat gctcatggta
     5161 ggattggtaa gcgtgaagtt ctgcgttacg ttggaggccg gcggaacgac aatgcctgta
     5221 gccgtcgccg agttgtaacc cggggccgtg acaatcatat cataagttcc gggaatcagt
     5281 tccagttcgt aagcgcctcc tgctccggtc atcgtattgt aagcaccggc atacaccgat
     5341 gccccgatga tcggattcag cgcactgttg cggacccatc ctgaaatgat ggtaggggtg
     5401 agcgttgtaa atgaccatac cgtgcccgta gtatttccac cggggccata gctgtcaacc
     5461 ctccaataat aggtggtggt ggcaagcagg tcgggaaccg gatcataagt agtcgccgat
     5521 cccatatcaa ggccattcac aaggttggta ggtgggttgt cggtgcccag gtacagcgtg
     5581 taacccgtaa acgggccgcc accggagcct ccgttgcccc agttgagggt ggtttgcctt
     5641 ggaacatccg tttctccgtt ggcaggcgaa acaatagttg ctgctgccgg agggaggaag
     5701 gtatacgtgt aagtgaaagt aagcccgttg atgggcggat acgtggcatt ggttgtgaca
     5761 gtctccgtat taaccgtacc ggctactgta ttgttccacc ccgttgttga agtacggtta
     5821 ctgaaatcgg tatttgtcag gcctcttaat ccgacttgcg gataattggt tcctgtgatt
     5881 gccgtacaag ggccgtaaat catctcaatt ttactggtgg tttcgtaaat cttcacctgt
     5941 aggttgagaa tttcacccgc gtaagcgctt ccgtaacgtt ttacgttttt ccactgcacg
     6001 gtgaaaaccc tgttgggtgc gacgcctgac agtaaataac ttaccgaaga agtagcgtca
     6061 atgcttcgca aatccctgcc aaagggtgca actacaagat atattcctgt aattgagctg
     6121 gagatcgggg tatagcccag tgaagaggtg gaagtgttcg ggaaataaag ccacccgttc
     6181 gctgaaaccc ttatctgatc tttactccat ccatttccca gatgggtgaa gccgagcgaa
     6241 acgacagccg tttgatcatc aaacggattg cctaatatat cggtaccagt aatttcatta
     6301 taggtgccgt ttgattgggc aaaagcataa taagtcgtct cctggccata agaattccag
     6361 gcagcaaatg ccaccaagaa aagcataaag aacaattttc tcatgttgcg taaattggtt
     6421 ttcattaggt ttatgttagt taattaataa gtgtcaggtc acaaaaaaac gatagcagac
     6481 actgccgggc agtgaactgt atcgttaaca tctttttttt gataacctgt aagagggaag
     6541 cataataata gattttaaca ttattggtac aatagttggt tttaggcttt aaatgtctgt
     6601 ttttcctcct ttctttccgc ttaatgcctg catctcactt gtaagagcaa actgatggtt
     6661 agtaattgat tgaattacag taattatttt gttttgatta taatttggcc aacgggtaaa
     6721 tcatcagagc cgtgtttatt tatcagcggt taaacagcct gaccgaatgc ttatatacaa
     6781 atataaggca ttcagctaca tattgtcatc cgggggagcg catacccgga tgatttactt
     6841 atgtatcttt attatagatg acaattaatc agctctttac cactattcgc cataacattt
     6901 ttttttgtgg gaaataattc aatttattta catttcccgg caaacaagca gtttcttatt
     6961 cttccggcga gccccggtcc agtaaatttg aatgaccgat gacttcagaa agcgccggtt
     7021 ttgcgccgtt ttttaccgcc aagcgatatg aaggctattc cttaattatt ttatatcgtg
     7081 tgacaatgcc gttatcacgt tgtatttcga taaaatacag acctgcaggc aatgcacgta
     7141 atgacaaatc caggggcagg gagccggttc cctggctcct gccggtaata ccaagcagac
     7201 gagagtatgt gtccctgagg ataatccgat aattctcccc gtcggccaaa ccgcttatcg
     7261 aaacaatatc atgggttgga ttaggggcaa caacaatatt ttccagcgaa ggatcataac
     7321 cctccggcat tgcaatacct attttaaagg gtaagatttt taccagtatc ccgctgattg
     7381 caggctgctc cggggtaagg ctgattaaag gagtttcatt cttctggtag cctgcttttt
     7441 cacctacaag gacataatcg ccgaacggaa ggttccggaa gttgaaattt ccttcgtcat
     7501 ccagacgctg ccatcccagg aggtacttca aggtcttatc gtataataaa atggtgacat
     7561 cgccgcaaag aaaaggcccc ccggtttcac aactaccgct tattgatccc gggccttccg
     7621 gaatctggtg cggcagtttg aataattcga tatccacatc ataagtattg gcttcggtat
     7681 taaacctgta agcattttcc cagttcagat cattaaaata ataggtcggg gcgtactgat
     7741 ctgccaggtt cacgggaggt attgcataga tgtaatattc accctcaata agaagatcaa
     7801 aaatgaaagt tccgtccatg gccgtcaggg tgcttctcat ggccgataca gtattgccgg
     7861 aacacctgta aaggttcagc gtactgccgg gtacaaggtt gccgccggca tgaacacggc
     7921 ctttaatgaa ataaccttcc acaatctgga ctttaattgt gtcggaagcc ggtccgcaag
     7981 gtgaagatat gtcaaggata aggttaacat ggccggaagc cagatcgccg gctcccggga
     8041 aataatcagg attctttaca gaatcgttac taaaggtgcc atcccccaga gtcaaccaat
     8101 gtaaaccggt aaaataatcg gccgcggccg aagaaagcag aagatggctg ccgctgatga
     8161 tggtagtatc ggaaccggca aatacgtaag gggcggggcg cacttttact ctcatttcat
     8221 catccaggtt gaagccttca ccggaaatgt gcagggtaag ataaacagcg ccgattgcca
     8281 catctccctg gccaggcgta taaacgggat gcagaatcct cggattatca aatgtcccgt
     8341 cgccggtggt tgtccaggaa agtaaattat aaccgtttgc cgtagcctcg cttaataaga
     8401 gttctccgtt ttcgcagact tcggaatttg gtccggccca gggccccgga attgctatcg
     8461 taaaatcgct ctcatggcct tcgggacggt caaacagacc gtcaaattcc gtatcgacag
     8521 cggtaacggc aatcgtgtca tccagcgata cgccctgcag aaatatactg gtgtcggttc
     8581 ccgcttccag cgaatactga taattaaagc cgtcgaatgt accgtaatga atccggtaac
     8641 cagccaggtc agattcagta ttgggaggcc agcttacctg tataccgttc cctacatcct
     8701 ttttaacagc ataagcagga gccttgaccg gggcctctgt atcagggccg ttatggaaag
     8761 gctgataatt taccaatcct ttggccggat catcaaaaac gtcagaaata aaggtattta
     8821 tggtgttggt atcgcttgtg ccccaccaat tgccaaccgc ttctattaat tgagcagtat
     8881 ggttaagaaa tgctgctgag tccctgtttt cataaatact attgctgaca aaaaggccct
     8941 gaggcaccga catgaaagag aaggcagtat cggcattatg atgcaccgaa tttgcaacaa
     9001 aggagttgaa caggctggaa ccggttttac caggcgtgaa cagaattccg ttttggttgt
     9061 gttccaccga gttggttttg atcaggttat cagaagcccc ttgttcaaca acaattcccc
     9121 acaggttgtt cccggaaatt aggttattgc gcacggttga ccggcaggcc ccgataattt
     9181 caattccgtt gatcaggcta ttcacgatgt agtttcgttc aataacggag gtgtcctgaa
     9241 acaacctgat tccaaatgaa ttgttattga aaatattttc acttacctgg ttataccctt
     9301 catcaacggg accgtcgttt cccaggtaca gcccgacaag gtttcctgaa acattgttac
     9361 ctgaaattgt attgtgcgat atctgcccgc tgctgttgtt tatgaaaaac ccgacatacc
     9421 ggttattggt cactgcgtta ttcagaaaca ggttgtattg ggctacatag gaggatgcca
     9481 tgaatatgcc aaaatcgctg tcgccgatcc ggcaattcct gatgacattg ttcagggcat
     9541 ctttcatata aatcccaaag gatgcccgtt tcaggcagca atgttctatt aaaatgcctg
     9601 tattttcctg gagaaccacc gaataagcag aattttcgaa acgggcatac gaaaggatag
     9661 tgccgccggt ataatttccc agatcatcga attccgtcag cgccccgtga aaatatatgc
     9721 cgttccacaa atcgtcttca ggaagaaaca tgatgctgtc ggagggagta ccctgtgcaa
     9781 tcagtgttcc tcttacttcc agcgccgtat tgacttcgaa caggacctta accccgggtt
     9841 ctatcgtcag ggttatccct ttagggacaa tcacagattg tacaacaatg taatcctgcc
     9901 ccgtttttga ccaggtggta ttttcggtga gggtatcgcc tacatatatc tgcgcgtttc
     9961 ctgaaagaga tacagttacc aggcataagg caatcatcag gttccagccc gctttattca
    10021 tcccgttcct catcgctttc gtaaatctaa gcaccatacc tggaatttat ttaatcataa
    10081 atcctgcatt aaatcaaagg taaagaatta gtaaatccga ttttaacgga gggtgctaat
    10141 tgtttttcac aattttatgc acataccggc cctgaaccct ggtaaccggt aagttttatc
    10201 gagcgggtaa tccgaataca acgatgaaac ctggcgcctg taaaacgctt ctgcctccag
    10261 cgaaagtttt ccgctgatcc tgaacccggc ccccaggcct gctatccatg aaaagacaaa
    10321 cgggtttaac ctgttcctgt ctatccattc agccttatag tcctccggtg aaacgaccag
    10381 cgctttcgag ccgatcagaa ttccggcaac agctcctcct ttcatataaa ggttaaagtt
    10441 ttcattcttt atgacaaaca aacgcaatga caggggaaca tcaagatacc tgaaagcatt
    10501 gggattggtg taagagaatt ttgttgagtt gaggttttgc caggtagaat cggtaatgta
    10561 atacctcaat gtatctgttc cctcaacggt ataataggtt tcaacggtat ccctgagata
    10621 atacccgccg gtttgttcga catattcatg gtcaagaatc tcacccagac gggtgtagaa
    10681 aaggcccgtg ccagccccta tccttttata gtcataggtt atggatatcc cggctgaaaa
    10741 attctgcaaa gaaaccgatt ccgattgccg tgatttttcc agtaactctg aaaggctgcc
    10801 ctgcccgccg gcataggtag ctttaccaaa atactgtgaa tagcttaatc caacagaaaa
    10861 accgttgttt tcctgaaatt tcctgttctt tacgagttct ttgtcaaaat tactgttacg
    10921 gtttcccttg atttttcgtg tcttgtattt ttcgataaat accgtatcgc gatgaactac
    10981 cgtatcgcga aatataaggg tatcgatgcg caggatggtt tccgtgtttg ttataaaaac
    11041 agtgtcaatc ctgaacctgg tagcatggct gatggtatcc ggtttcaggc gtattgctga
    11101 atttttattg agatacctct gagcttcggg cttcggtttt tcagcagcat tgtcagcttc
    11161 agcagccggt ttgaaaatga ttacctggtt ccccattgca cggaacgaaa gcgccggatt
    11221 attcagcagt tgcacgagaa ccttgttcag cggttctctc ttagcctgga tgctcaccag
    11281 ccggcgggcc gagagttcgt caggatcata ggaaaacttt atggaagcct gcctgccgat
    11341 tttattcaat acttccgata aaggctggtt tacagcggta acagttacgg gccggtttaa
    11401 tacagaaagc tgggcatcgg ctgatgcgac ggtgataagc aggaatgaaa ttattatgaa
    11461 acttctatac aaagccttcc gataaggtac gatcatctga caatccgttg attagcctgt
    11521 ttatttagtt accaggtaat cctttcctgt ttttacaaat tcaacggagt gcactaatcg
    11581 gataacttca aggacagaat cgagcgactg atctttgtca aaagtagcgg taagcctgag
    11641 gtttttaatt tgcttattct ttatgtcgaa tgatacgcca taccggtttc caattgcatc
    11701 aaatacatcg cccagcgtgg cttcgtcaaa taccagaatg ccggttttcc aactcatgaa
    11761 attcaggtcg gtattgacgg ttttgaaaat gcgtcctgct ttccggtcat aaataccctt
    11821 atcgccaacc gaaagggtaa cctgacccag tgcattttcg tctttatcca cataatagaa
    11881 aagaactgaa cctgttttta ccgtaacttc aacctttcct gtttcctcat aatccttaac
    11941 gttgaatgaa gtccccagca cttttatgcg ggtttctccg gcatttatga taaaaggcct
    12001 ggttttgtcc gaagcaatcg aaaaaaatgc ttcgccccaa aaatagacct ctctgttgtc
    12061 atgtccaaat ttttcaggaa atttaatttc agaattctta ttcaggtgga tgacggaacc
    12121 gtcggcaaga tttacaggta cggctattgc cgaaccagaa gcaaaagtgt tcaggtcatc
    12181 ggacgggcgt acatattgaa aaatccccaa tcctatcagt aaaatagctg cggcgcccga
    12241 cactgcatat acaattttcc ggattggatg cgggtgcagg gtacgtatca ccggttcctg
    12301 ttttgcatca atgcggttct gcacctttgc ccatgcctca tcaacgtcat aagtttcttc
    12361 tccggcagaa ggacgggttg aatgccaggt ccgctgcaaa tgcctgtaaa tgctgcggta
    12421 acgtttgtcg gtattgatga gttccgaaat aatgcgctgc tcttccccgc tgcattcgtt
    12481 tgcaaggaat cgcacaatct gcatttttat ttttcggttc tcgaacatag ttatgggaac
    12541 agtttttaca acttttcaag aatatgacaa acaaagccgc ctttaccctt actcatttta
    12601 taaatttttt ttaatttttt attatcatgc ttataatcag cacaataggg gttacatatt
    12661 catgaagata tttgcgaaga aattccagcg cttttaccat ttgtgattct acggtcttga
    12721 ctttgatttt catcctgtcg gcaatttctt catagcgtaa tccttcaaaa cggctcatct
    12781 cgaaaatttc ccttctgcgt tccggcaacg aactgatgcc cagactaatc ctgcgctgta
    12841 attcctgctg gaccagatca tcggtatcgc tgtatgcttc ttcaacctta aaaccgatat
    12901 aatcgacata tttacggtct acctccttgc gttttacggt attcagcgca tggttaatgg
    12961 tagccctcag cagatagttt ttcagggagg tattgataac gagttccgac cgccggaacc
    13021 agatcttgca aaacaggtcc tgtacaactt cttcggaagc cgatttatcg gaaaggtaac
    13081 gattggcgta ggaacacatc gggacgtaat atgtcctgaa gatatgttca aacgtcggcc
    13141 tgtcgccttg tttgagttta tcgatcaata tcttttcaga ttcttccatt cagttttcct
    13201 gttgttgact tccggaaaaa atagccctga agaacggcaa taaagataca attttaattt
    13261 tagtccggtt ttagtatata tttttcgtca ggagttgtgg cccgggcata ttgtatccct
    13321 tcgcgctccg gccccggatc tttggtcccc ctgcacctga tgcgaccagt cctgaaagca
    13381 ggagtgccat tccaagttgc gccagcgcga caaggttccc gaaggcagaa ttcttccctg
    13441 caaggctatc ggttttgccg gttccagtcg ggtggtttcg gcatacgtct gccacaggcc
    13501 cccgatatct ccgctaaagc gaatatttgc tttcatcaca ttggcatttt cgataaattt
    13561 gccgtcacaa tagaggcgga tattgctgat gaaccataaa aacatggttt tgaaaggggg
    13621 aataacaatg gggaataacc gccggaatat gcattcgaac cataaagagg tcctcccggg
    13681 atattttttt gttgttcaca tgttgttgtt aataattttt ttctaatttt gcacctccaa
    13741 ttttgtcagc aaaatataag aaactgaaaa tgtacgcgat agtagatata gccggacagc
    13801 aattcaaggt taaaaaagat cagaagatct ttgtttaccg ccttcagggc gaagaagggg
    13861 catcggtcga gtttaaagat gtattgcttc tcgacaatga aggcagcatc agcataggaa
    13921 ccccccacgt tgaaggagcc ctggtgaccg ccaggatttt aacgcacacc cgcggtgaaa
    13981 aggtaacgat cttcaagaaa aaacgccgta aaggctacca gaaagaatcc ggccatcgcc
    14041 aggatctgac acagttgcaa attgaagcca ttacggctta attttgcacc ggaagttaat
    14101 ttgatttaaa aacaagtaat acaatcatac aatggctcac aagaaaggtg ccggaagttc
    14161 acaaaacgga cgcgaatcac acagcaaacg cctgggcgtt aagatttacg gcggccaatt
    14221 tgcacaggcc ggcaacatta tagttaggca acgcggtacg gttcattacc cgggaatcaa
    14281 cgtagggctc ggtaaagacc atactctttt tgcactccgc gatggcgtag ttgaattcaa
    14341 gaaacgcagg aacgaccgct catacgtttc agttcatccc ctggatgctg aataaattca
    14401 taaaagctgc aacagcaggc tttacgcaat aatattcatg aaatctcctg accggaaatc
    14461 aggagatttt tatttttgta ctgcctaaat gtaacccggc ccggattccg ggcctgtacc
    14521 gatgaaacct gtctctttgc aattgtaata accataaaga agatgttaga actttcctta
    14581 cttcgtgaaa acccgcaact ggtcatcgac cgcctcatgg tcaagcattt cgatgccaaa
    14641 gatatcgtcg ctaaaattct tgatgctgac cagtcgcgcc gtgaaaccca aaagcttctc
    14701 gacgacaatc tggctgaagc caataacctg gcgcgcgaaa taggagttct gtttaaaaac
    14761 ggcagacaac aggaagccaa cgacctgaaa acaaaaacag cagagctgaa agcaacggcc
    14821 aaaaacctct cggaaaagct tgatacgttg cagaaagaac aggaccgcct gctggtaatc
    14881 ctgcccaacc tgccgcatcc cgatgttatt cccggaaact cctccgaaga taatgttgaa
    14941 gttcaccgcg aaggcgacat cccggttttg ccggataacg ccatgcccca ctgggattta
    15001 tgccagaaat atgacatcat tgattttgaa ctcggcgtta agcttaccgg agccgggttc
    15061 cctgtatata aaggcaaagg ggccaggctg caacgcgcgc tcatcaactt cttcctggat
    15121 aacgccatcc gggccgggtt ccatgaaatc cagccgccgc ttatggtgaa cgaagcctca
    15181 ggttacggaa caggtcagtt gccggataaa gacgaccaga tgtattttat cgggcttgac
    15241 aagttttaca tgatcccgac cgccgaagtt cccgtcacca atatttaccg cgacgtgatc
    15301 ctgaatgcaa ccgattttcc ggttaaaaat gtagcctatt cggcctgctt ccgccgcgaa
    15361 gccggttcat acggcaagga tgtgcgcggg cttaacaggc tccaccagtt cgataaagtg
    15421 gaaatcgtac aaatacagca ccccgataaa tcatacgaaa cgcttgaaga aatgcgcgaa
    15481 tatgtagccg ggcttgcccg tacactggaa ctgccgttca ggatcgtcag gctttgcggg
    15541 gctgatatga gttttacctc agccatgacc tacgattttg aagttttctc ggccgcacag
    15601 aaacgctggc tcgaagtgag ttcggtatcc aacttcgagt cattccaggc aaaccgcatg
    15661 aaactccgtt ttaaagaagg aagcggcaaa acccgcctag cccacaccct gaacggaagc
    15721 gcccttgcat tgccgcgcat agtggcagcc ctgctcgaga acaaccaaac accggaagga
    15781 atccgcattc ccaaagcgct ggtgccgtac acaggatttg atatgattcc ataaaccgga
    15841 taatgccgga aaaataagaa tgctgttccg gtgggtgatc ctgtaattaa aaattaccgg
    15901 ttttcggtgt tacgatggta agaaattacg gatcctgatc gttaatactg tcgtgatgaa
    15961 aactataagc tgcaaccggc tactgttatc agccttgttt atggttctgc tcaggcctgt
    16021 tatggcacag cagcaaaccg acgaacggct gggcatggaa tattacagca cccggcaata
    16081 cgataaagcg atcgaggtct tcagccgtct gtatgaaaag tcccctaccc ttttcagtta
    16141 catttattat gtaaactgcc tcttcgaagt taaggattat gataaagctg aaaagctgat
    16201 caggaaacag atgcgtgcaa atcctaacga tccgcgctat acggttgacc ttgggtacct
    16261 gatggcaata caggatgaaa cacaaaaagg caaaaagctt tacgatgaat gcctccgtaa
    16321 cctgaaagat gaccgcaact ccgtttataa taccgctgcg gccttcctca accggcgcga
    16381 aactgattat gccatccgca cctacctgaa aggacgcgac ctgcttaaag acaaatcggc
    16441 atttgccatc gaactggcca tacaatatga aagccagggc aattcggcag ccatgctgga
    16501 tgaatatctc accctgctgg ccgacaaacc cgaacaaatg gctacggttc aaaaccggat
    16561 gcaagcctgg ctgagcgacg atcccgacaa tacgaaaaac gacctgtttc gctccacact
    16621 gctgaaaaaa tcacagcaaa accccgatga agtttcattt gccgaattgc tgctatggta
    16681 ttcggttcag cagaaagatt tcaaacttgc cctgttgcag gcaaaagcgc tcgaccgccg
    16741 ttacggcgaa gacgggcaga gggtttacga ccttgcccgg ctaagtttta ccaacgacga
    16801 tttcgacgtc accaccgacg cttatgctta tatcatcaaa aaaaacgccg atgccgatct
    16861 ggtcctgaaa agccgcatcg aattgctgaa caccgaacta gcaaaagcca ttcagcttca
    16921 cagttacgac gataagttcc tcaaggcgct tgaagcaaaa tacctttcgc tgttaaagga
    16981 atccggcaaa aacccggcga cgattcccct gatccgcaac ctgagccatt tgcaggcatt
    17041 ttacctgaac aataccgatt cggccacggc gctgctcgaa gatgccatca tcataccggg
    17101 catcaccgac cggctgcagg ccgaatgcaa actcgaactg gccgatattt accttttctc
    17161 gggcgaaccc tgggaagcca cgctcctgta ttcccaggtt gaaaaagctt ttaaaaatga
    17221 accgatcggc catgaagcta aattcaggaa tgcaaagctg tccttttata tctgcgaatt
    17281 tgaatgggcc aaaagccagc tcgacatcct gaaagccgcg acctcaaaac tcatcgccaa
    17341 cgatgccatg tcgctgtcgt tgctgataag tgaaaacatg gacgacgaca gtacttacgg
    17401 ggcgctttcg atgtacgccc gcgccgattt attgctgttc cggaacaggg accaggaagc
    17461 catggacgcc ctcgactcgc tcctgatcag gtatccgcac cattccctgg cagatgaagt
    17521 gctctttaaa aaagcggaaa tctgcatgaa ggagaagaaa tttgaggatg ccgtgaaata
    17581 ttaccgcgaa atagtggctg acttccccac cgaccttctc gccgatgatg cattatatgc
    17641 ccttgccggt atttatgact acattacccc ggacaaacaa aaggccatgg aactttatca
    17701 gaccttgctc acaacatacc ccggcagttt atttgtagtg gacgcccgga aacggttcag
    17761 gatcctccgc aacgatccgg tgaacctata gtcattgtta aataaatcaa tagtatggaa
    17821 ccggtcaggg ccaagaaaca cctgggacag cattttctga aagacgagaa tatcgcccgt
    17881 aaaatcgccg aaagcctctc gcccggtgta agccaggtac ttgaagtagg ccccggcacc
    17941 ggcgtcctca ccaaatacct gattgcgaat cactattcct cgttcaaagc cattgagctc
    18001 gaccgggaat cggccgctta ccttaaatcg gcatttacac tgggagaaga cctgatccac
    18061 gccgacttcc tgcgaaccga catttcagcc ttgtttgaag ggccttttgc cgttatcggc
    18121 aacttcccgt acaatatttc gagccagatt tttttcaaag tggtcgaaaa ccgggataag
    18181 gtttccgaat gcgtcgggat gatccagcgt gaagtcgccc aacgcatggt ttcaaaaccc
    18241 ggcaccaaag attacggcat cctcagtatc ctgctccgcg catggtatga tatcgaaata
    18301 ctgttcatcg tgccgcccca ggtatttgtc ccgccgccca aagtcacgtc ggctgtggtg
    18361 aaacttaccc gcaacggcat cgtggacctg ggctgcgaca caaaattatt cagtacggtg
    18421 gttaagaccg ctttcaacca gcggcggaaa acgcttcata attccctgaa accgctggca
    18481 gattataacg gggaatttac cggcaaacgc gccgaacaat tgtcgatcag cgattttgtc
    18541 acgctcacga acgatatagc tgaaacttgc agccagtagc attcctgaac tttcatgccc
    18601 ggtatttcaa gggtatttat actgttattt cacatctttt aaattgatta tttttgccgg
    18661 aaacatttga caaggtatga acagcagtaa ggataaaccg ttccggtcgg cgcttaaagt
    18721 aaacgaagga attgaacagc ctcccgtggt aaacgaactt gctgcgtcca ggttcaggaa
    18781 ggcccggcac aatcttctgc ctgccggcga ataccttgac gggatactat cgggcaaccg
    18841 gaccatcctc agcaaagcca tcacgcttat cgaaagttcc ctgccgcagc accaggaact
    18901 ggcgcaggag attattgcgc attgcctgcc cctttccggg aattcggtcc gcctcggcat
    18961 taccggcgta cccggggtag gcaaaagcac ctttattgaa gccttcgggc tttacctcat
    19021 ttctctcggt cataaaccgg ctatcctggc tattgacccc agcagcagcc gctcaaaagg
    19081 aagcatactc ggcgacaaaa cgcgcatgga agaactttcg gccaacgaaa acgcctttat
    19141 ccgtccttcg ccctcggcgg gatcgctggg cggcgttgcc cgcaaaacac gcgaaaccat
    19201 tatcctgtgc gaagccgccg gtttcgatac catcatggtc gaaacggtgg gtgttggcca
    19261 gagcgaaacc accgttcatt ccatggtcga ctttttcctg ctgctgatgc tcgccggcgc
    19321 cggcgacgaa cttcagggca ttaaaagggg cattatggaa atggccgatg ccatcttcat
    19381 caataaagcg gacggcgaca accttcaaaa ggtgacgatc gccctggccg aagtacgcaa
    19441 tgccctccat ttcttccctc ccgccgaatc aggctggcaa acggttgccg gcacctgttc
    19501 cgccgttacc aggtccggaa tccccgccgt atggaaagcc atcgaagatt atctttcatt
    19561 gacgcggaaa aacggctatt tccaccatcg ccggcttgag caggcccggt ttaccatgca
    19621 ccagtctgtt gaacaacagt tgaatgaccg gttttaccat gacccggaga tcaaaaaact
    19681 cctgcattcg cttgaacacg acctgtcgac aggcaagctc agcgcgtata tggcagcaca
    19741 aaagctgctg gatgcctggg accggaaaaa caaatagcta ctgctcctct tcagccattc
    19801 accggaacag aaatctgcga aaggcaaaat tcagtaaaac acgtaaagag catcttcagg
    19861 tagaaaaaga ttcctacttt tgccgtccct actatctcat ttaacgcatg aaaaatcctt
    19921 tcttcaacag ccttactgcc atgaacccga attcgctggt tcaatatctc aataaaccca
    19981 gcgacgactt taccaagtct gacattatcc gttacatcga cgatcacaat atttcgatga
    20041 tcaacttcag gtacgcaggt ggtgacggaa ggctgaaaac tttaaatttc gttatcaaca
    20101 gtcaggagca tctcgacagt atcctttcga ccggcgagcg ggttgacgga tcaagcctgt
    20161 ttccctttgt gaaagcaggc agcagcgacc tgtacgttgt accccgctat aaaaccgcgt
    20221 ttctcaatcc tttcgcccag gttcccaccc ttgacatcct ttgctcttac tacgacaaag
    20281 acgggaatcc tctggaaagt tcccccgagt acatcctccg taaagcccat aatgtgctga
    20341 aagaccgtac cggcctcact tttcatgtga tgggcgagct tgaatattac gtgattagcc
    20401 agaaaaacga gcttttccag gctgccgacc agaaaggcta ccacgaatcg catccgtttg
    20461 ccaaatggga agcgctgcgg gccgaagcca tggttgccat cgcccagacg ggcggccaga
    20521 taaaatacgg ccattcagaa gtaggtaatt tttcgctcaa cggactggaa tttgaacaaa
    20581 acgagattga attcctgccc gtgaacctcg aagatgccgc cgaccaactg gtcatcgcga
    20641 aatggatact tcgctcgctg gcatacaaat acggcgtttc gttgaccttt gcccccaaaa
    20701 tcaccgtcgg aaaagccgga agcggcatgc acatccatac ccggctcatg aaagacagca
    20761 ggaacgccat ggttgaagac gggcaactca gcgatacagc ccgcagggcc attgccggtt
    20821 atctcgatct ggccccttcg ctcacggcct ttggcaacac caatccaact tcctatttcc
    20881 gcctggttcc gcaccaggaa gcgccgacca acatctgctg gggcgaccgc aaccgttcgg
    20941 ttcttgtaag ggtgcccctt ggctggtcgg gcgccaacaa catgatcaac cacgccaacc
    21001 cgcaggaaga aaaagacgaa aaagattaca gcgataagca aaccgtggaa ttccgctgtc
    21061 ccgacggctc cgccgacgtt taccttttac tggccgggct taccgttgcc gcccgccacg
    21121 gactggaaat gaaggatgcc ctggagtatg cccggaaaac ctatgtggat gtgaacattt
    21181 tcgacgaggc ccacaaagcc cgcgtggccg aactgaaaca actgcccgtt tcgtgctggg
    21241 attcggctga aatgctgctg aagcaggccg atatttttaa aatgtacgac gttttcccgc
    21301 agtcggtgat cgaaggaacg gccaataaat taaagtcgtt cgaagacagg ggcctgcgcg
    21361 aaagcctgga aggcaacacc gaaaaactcc aggagatcgt gaaccggtat tttcattgcg
    21421 ggtaggaaaa taatcctcta ttgataggca tttctaagcc agtgccatta ataaacgctc
    21481 aatcctaaaa tacaacaaca gtccggttag gtttttatga cctgaccgga ctgtttgaaa
    21541 aatacccgat tctacctgaa tcaatctttt tcttaatcaa cttacctgat cacgcacatt
    21601 tttcgtgttg caacatcact accatcaatt ctcagtgtga agaagtaaaa accggagggc
    21661 aatgtctgat tgaaaaaacc tactgaatag gagccctttc cctgtgtgcc ttgcggcaaa
    21721 ctgagaatct cctggccggt aaggtttctg actgaaatat caacatttcc tgcccttgca
    21781 atggagtaat tgatttgagt actttccgaa aacgggtttg gcgtattctg gtttaagaag
    21841 ccccgatttt ttccggcgat atctgaatcc g
//
LOCUS       DAOPYV010000052        21518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_2499_length_21518_cov_11.895495, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000052 DAOPYV010000000
VERSION     DAOPYV010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21518
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2499_length_21518_cov_11.895495"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(405..1940)
                     /locus_tag="PKI35_07910"
     CDS             complement(405..1940)
                     /locus_tag="PKI35_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNX44342.1"
                     /translation="MTTELNKLGIQELNSGASTGSQWLATKGDVTSSCSPHDGSLIAR
                     VNNATIEDYEYLMEKAGDAYRQWHIVPAPKRGEVVRRIGETLRRHKKDLGYLVSLEMG
                     KIYEEGLGEVQEMIDVCEFAVGQSRLLNGATMHSERPFHRMYDQYHPLGIVGIVTAFN
                     FPVAVWAWNSMLAAIAGDVVIWKPSSKTPLCAIAVQKIISETLVECGVPEGVFNLIIT
                     RSEVLGDNFAADKRIPLISVTGSTAVGKRMAAIVGQRLGKTIMELGGNNAVIISEHAN
                     LDMTMQSVVFGAVGTCGQRCTSTRRLIVQEKVYETVKKRLVAAYSSLTDRIGDPLDKN
                     TLIGPLVDNQAVRNFTRALEEVQKEGGKVIFGGQVLSGGKFASGNYVVPAVVETENYY
                     KMVQEETFAPIVYIIRYKTIEDAIALNNNVPQGLSSCIFTENLAEAEKFLSETGSDCG
                     IANVNLGTSGAEIGGAFGGEKDTGGGRESGSDAWKAYMRRQTNTINFSGQTALAQGIT
                     FDF"
     gene            complement(1961..3589)
                     /gene="pruA"
                     /locus_tag="PKI35_07915"
     CDS             complement(1961..3589)
                     /gene="pruA"
                     /locus_tag="PKI35_07915"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNX44343.1"
                     /translation="MNNAIYNFPQPQNEPVLDYKKDSPERMLLNAELKRQMQEAIEIP
                     LIIGGKEIRTNETGLVRMPHKHAHVLATYYKASEKEADMAIEAALNAHKIWADLSWTV
                     RASILLKAAGLITGKYRYILNAATMLGQSKNIYQSEIDVVCEATDFLKYNAYFASRIY
                     EMQPRSAFNQLNKMEFRPLEGFVFTVSPFNFSSIASNLNMAPVMMGNTTVWKPATTSL
                     LSNYYLMKIFQEAGLPDGVINFVPGSGALIGKRVLQSEDLAGIHFTGSNQTFNSLWKE
                     VGSQLSHYKSYPRLIGETGGKNFIFVHPSASPLEVAVNAFRGAFEFQGQKCSAASRMY
                     VPVSLWPSVKTEMLKMCADASMGDVMDPMNFINAVIDENSFDNIMEYIHFAKSSKEAE
                     IIAGGKGDKTTGYFVEPTIIVTTNPKFRTMEEEIFGPVLTVYVYEDDKLQETVGLCNT
                     TSPYGLTGAVFARDRIVASVLCEQLQYAAGNFYINDKPTGAIVGLQPFGGSRASGTND
                     KAGGEFNLIRWISPRTIKETFVPATDYRYPYMID"
     gene            complement(3684..4058)
                     /locus_tag="PKI35_07920"
     CDS             complement(3684..4058)
                     /locus_tag="PKI35_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX44344.1"
                     /translation="MSSRLDNLRLNYKIWLETSDRQGILGDKKCELLKAIEETGSLNE
                     AMKKLNLTYRKTWDNLHKIERELGFPLIKPTRGGAEGGNTILTVEGKIIVQAFEKFHS
                     EFDLIIQQGFDRIMNDLKDQIK"
     assembly_gap    4235..4334
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4560..5420)
                     /locus_tag="PKI35_07925"
     CDS             complement(4560..5420)
                     /locus_tag="PKI35_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44345.1"
                     /translation="MKSIKYALIAIFLCGIIATGFVTVPEKKQVIFLQSAESNISPDQ
                     MNKAAEVITLRLKNFLSGPFDVKAVSGRNQIRITVDGNAELKMIKSLITQKGMFSFYK
                     TYNNQSLKDLVKDDKNLVNMLLERGNAASSATLLCTSADSIGYFERYLAPSSVNEEVR
                     FAWSNLFDEQDACLYALQTGKENIIPLTGADIESFETKQDAQARWQSIGFRFREPVIG
                     VWADITKSNLGKAIAVLMDNKVLFAPVVQSEITGGNLEITGDFSQTEVRFIAAVGKSG
                     TLPVSLVVVK"
     gene            complement(5492..5884)
                     /locus_tag="PKI35_07930"
     CDS             complement(5492..5884)
                     /locus_tag="PKI35_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44346.1"
                     /translation="MTRQEGNREIFFGSAVIVLLFFLFVLVFSNKPDNRIENAGQLRS
                     EYCTQYQLVAVNYTLEFRCLNNSIPVYDRKNFKTFGNSFKTAAENRLLQHSIRFHEKA
                     ERLIKPVILQRFYYQYHTIEADDLPFLS"
     gene            6859..7356
                     /locus_tag="PKI35_07935"
     CDS             6859..7356
                     /locus_tag="PKI35_07935"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HNX44347.1"
                     /translation="MKKLFFVTAIMLGFAAGAFAQVSATASAEATIVTPIAITKTVDM
                     NFGNVAVNSTAGTVVLATDGSRTTTGGVTLPVTAGSPAAASFTVTGTAGYTYAITLPG
                     ATTIANGVITMGVDTWTSSPDATGTLTGGTETLTVGATLHVGAAQAAGVYSNATACPV
                     TVNYN"
     gene            7508..8332
                     /locus_tag="PKI35_07940"
     CDS             7508..8332
                     /locus_tag="PKI35_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44348.1"
                     /translation="MVLFLAGISAVAVFTTSSAFAQGNLILLQKRVVFEGGLKNATIE
                     FGNIGKDTARYVVSMLNMRMKEDGSFENITEPDSGQYFAEKYVRFFPRNIVLAPNESQ
                     TMKIQLVNTRDLQPGEYRSHLYFRAVPNMQDKQEAPAGNKEGISISLKPVFGITIPVI
                     IRAGEPNTTVKLSDLALVQQNDSTQAVNFTIHRSGNFSVYGDITATYIAADGKEIEAG
                     SVKGVAVYTPGTLRTSRLALRNIDGVDYHNGKLVVKYARQAEEDDAVMAEAELKLK"
     gene            8364..10214
                     /locus_tag="PKI35_07945"
     CDS             8364..10214
                     /locus_tag="PKI35_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44349.1"
                     /translation="MGHKTHGKEIQRMKTITGKIYLFTLLITLTASLYGYSQGVAINA
                     DGSAPDASAALDINSANKGLLIPRVALTSTSSNEPVGSGIATSLMVYNTATVNDVIPG
                     YYYWDGNSWIQFNAPETGSQSLNIVYKTSSGPIAKTETMVIATGDITLTLPAVTSADN
                     GLQITILNAGTYKDLVVVKGAAPGVTIHSVDSVLFYRGYSKTLVAVNGIWAKKEHMAL
                     QDNVFEISNKSSWNTIGEAVAFLKEHMTGPSVIRLGGGTYEIDTTLTINLPYPVTIEG
                     VSYGEAIIQGSAGLSGHEMFNCQSECYFKMITFQAVSNTSGNDGIHLTGSGEYYEIKD
                     CEFDGFYKGIAVTRNCEMWLFETDFYDITSIAVSVEDASGSSDGPVIKMSETDYTNCG
                     TGIKLVKGKNGIVDIVNCTFYNSNAGQTGILYVPGLGNFQNFTSMFITNNSWNNIGTF
                     MSGFDFTRADGRDVNAFIENNAGMGNKNPYVKVNVISNTASTDLPDSRYYYQANFVNT
                     SSFPCKWKVENNKITYLPANKKDVVMTVAGNIAGTSGSYITIAVGKNGQTVYAPITIR
                     SRTNGTYFAFTTVAYIENVSQGDYFEIYINSSSSFDDAILSDLNWWVDTK"
     gene            10240..11976
                     /locus_tag="PKI35_07950"
     CDS             10240..11976
                     /locus_tag="PKI35_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44350.1"
                     /translation="MKIRIKLFLLVYKLLLIPCLVSGQGMVISSGSHVVVGTGYVGIF
                     GNLTNNGSYTDQGGTLVLTGNTQNLTGTNGATLNNLNVSSGSTSTLSTAGQTVKGVLT
                     CDGTLNAGGNLTLLSTADQTALVSGAGAGTINGNVTMQRYLASGFGYKYFSSPFQAAT
                     VSEFADDMNLGAAFPTFYSYNENRDTTWWITYTTGTNLLVPMLGYAVNFGPSADPKTA
                     DVTGAVSNGTMSLTLYNHDKNHTKGFTLVGNPYPSPVDWDAATGWTKTNIDNAVYYFN
                     AGSADQYVGSYSTYINGVSSDGIANNIIPAMQGFFVHVTNGTFPVTGTLGFSNQVRIN
                     TLNPVFHKNGLLDYRPLIRISAGFEGAENRLDPLVVLFDGEASPLFNERQDAVKLMNT
                     DPEIPSFYTLSNDNIRYAIKSLPEPADSLTVIPLGITTQQQGIIDFAVNALENQQPGL
                     QLYFSDNKNRAVLAVNSNTRYQAFLDEGVCDGRFSLILSRHEIPTSSGSADELDAYYS
                     DGKIYVYCDFLNNEQGELQVSDMLGRIIHREKVTGIGYNSVNLNVPTGIYIASLRSEH
                     ASSSKKLFIPGK"
     gene            11973..12488
                     /locus_tag="PKI35_07955"
     CDS             11973..12488
                     /locus_tag="PKI35_07955"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HNX44351.1"
                     /translation="MKHRCLKLLSLLPSLKGAIMLIMIVSPVILMGQVNPPRPITLTV
                     SPVQNLNFGSFCITGANGGTVTISNTGVRTSSGDVFLVNSGYTEGIFTIEVATGTSLV
                     MMNGADATLTGSNGGTMTLTLGQTDPPLPYGTITPSTVFHIGATLNVGPVSNNPPGSY
                     SGTYEIIINHE"
     gene            12514..16536
                     /locus_tag="PKI35_07960"
     CDS             12514..16536
                     /locus_tag="PKI35_07960"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNX44352.1"
                     /translation="MKKTYLKERTGRILRFAAGLIVCALSVFAAPSVFADNTDVEPAF
                     DEVSLTLKVHKSATVEIPAVISGSTAYLSVSDLFTFLKIKNIVSDESNVISGTFLNES
                     DPYLIDKTRNIIRYRDKEYLLTHNELIRSESGLFLATNLFQEIFGLECTFYFRSLSVD
                     LDSKQELPLFREMRQEEMRKYIRKVKGEIHADTLIGRQRPMLYAGTADYAVYSTQATD
                     GPATTRASLSVGGFLAGGETTLGLNYYTDQPVTGRNQYFRWRYVNNDNKALRQVVAGN
                     ISTPSIASIFSPVIGVQLTNTPTTYRKFYGSYTLNDRTEPGWIVELYVNNTLVDYAKA
                     DASGFFTFQVPLAYGNTEVKLRFYGPWGEEKSKVQNIVIPFTFLPKNELEYTVSAGMV
                     EDSTNALFWRSSVNYGISNKISIGGGNEYLSTITTGRNIPYLNTSFSLTGNLLCNAEY
                     DFGVRKKGLVSYRLPSNFIVELSYTKYDKDQKAIFYNYLEEKKGSISLPLRFRNFSIY
                     SRLSVNHVSLKESMKLTLSEFIVSASYKKFSGNIHSYGYFFNTGTPVMYTDLSLGYRL
                     TKGISITPTVRYNHTRNQVTGLKCIAEKQLWGRGYITMTYEQYTDYNIRNLQVGIRYV
                     FSKAQAAVYANHHNGSVSFSEAVSGSLVFDPATSYVKAGNINKVGRGGITVLPFLDIN
                     CNGRRDKGEPRVSGLNVRVITGGRVEVSEKDSLIRIFDLEAYTNAYLEMDGAGLGSIS
                     WQLYKKTYSVPVEPNLMKLVEVPVTVVSEVSGLVKMKTSGQAIRRENIKINIYRDDKV
                     LVTSCFPEDDGYYNYMGLTPGNYTIIPDTARLSGMQMSVVPASRKLSIPESREGEVFD
                     TLDFIVESMKPAAGEVIPNPAEYTPTVAAAAPDTTTRRPGKTGPEQIIITDTSALNNT
                     AKAAVTTKPETPAPAIEDLTRVASDPASAISGSEIDDLPGYYVVQAGAFKKQFNAKNV
                     KKHIASQYNEKEVAIVSAQGYYKVLVAGYNSKAEAMAMRNSLRENGYPEAFIRKLSGG
                     IAIRAFGDLNSNGIFDPGEPCVNGLTASLSTGGILEVSPADTLIRIFSFEADSGAILE
                     VDFSANESHGWELRRKTFTVETDPVRMKLLEIPVYAPGMISGTVTAEGPAGANMPEMM
                     RIQIYRADSTLLTTLDVPENGRFSFKGLKPGHYRIQPDTLQLQKLRLTSMPAGREIKL
                     TEGRFGESVTGVDFALAPEVQSRVIPLQPNVYPELAVSDSALLIKPDRKNEIVNGCFA
                     IQLGAFQEHSNARSVSKRVSFRYHLKVDIIQTAGYYKVLIPGYYSRLDATMMLNKLRK
                     NGFPGAYLIYIPDRTGSEQP"
     gene            17643..19103
                     /locus_tag="PKI35_07965"
     CDS             17643..19103
                     /locus_tag="PKI35_07965"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNX44353.1"
                     /translation="MIFSKNIKLKMRPLDYDELLQFKNPFIAHLTIFNGIFVVVLQVN
                     DHEVNYFLEGKNYVTLSKEEFLKVWNNIVLIAYPSAQSCEPEYRQHHQEYLFKKVKLP
                     LLLSVLGLILIIQFVSNFSAMQLLPLLATKAIGLFFVSLLIKHELGIHSEMVDKLCTM
                     SKSAGCNEVLKSKGAKLFGKVLLADLGLVWFVSSFIFIFIHLFTSKTDYENIFNLLGW
                     LSIASVPFILFSVSYQAFIIRKYCPLCLGVMSTLIVDVLLLIAMYGFRFAIPSYSCIL
                     LMISILSVVLYVWLLIKNYYTRANTLDDIENKYLHLKRNPSVLQLELQHSENIEGSNF
                     PNPLRLTENNSEILLTEVINLYCSPCKQVIEKIDKLLQNCNGNCPDLQIVLLANAKDK
                     ADVMTITAAHLLSIADYLTPNQRFIALREWLESMDYNKWSQKYPVEISDRQFAMLKEN
                     VAWFKEHKVHGTPTAFLNTKKIPDNFDITDIQYMIN"
     gene            19270..19488
                     /locus_tag="PKI35_07970"
     CDS             19270..19488
                     /locus_tag="PKI35_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44354.1"
                     /translation="MKLNKDEMKKVLGGTPGGGGSTCKITCKNNDTNQIYGTLEVSKC
                     NDNDLTKCINAYPDTTVAVCGCSGDPQS"
     gene            19536..20975
                     /locus_tag="PKI35_07975"
     CDS             19536..20975
                     /locus_tag="PKI35_07975"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX44355.1"
                     /translation="MGFLEFTLRIPCLKKTMKLYIQLLAILISLSCNINCCLCQSNTT
                     ISGIIQNGENRFIDLSCDDFLFFERQEIAKTKIDDKGNFSFNFNIAQPMKVRLFNKLF
                     YICPGDSVNFYIEGDMYDPLKIHVSGTNSNPYNCLILLDSLSKSLPFKPYFYNFKLGL
                     SNYLDSININKKAMLALLSQYSDEFYLPSDFLAYASSKIVYDFYYQLMFPIISRNFPI
                     DQIPLAYLNLLNEISLNYDSLAKDRAYAFAASQFIRFKEKSTTYNSFDIINMHSTGLT
                     KELLLTDHAFSLVQNYSSKDSTQISLTIQTIDKEIKDKEFRKYFSIAKEKLGKYLHPI
                     PEEVLKTVLIDSSGNLLTFNDLLDKSSNKIIVLDFWASWCGPCIHGMPLFNKLSLNYG
                     KSDMLFVFLSLDKSDSDWRGGLSKTQIPGNHYHVLNNFKSDLAKFLDIRTIPRYVILN
                     RQHMLEKLEARDPSEGNGLSKQIDNLLLN"
     gene            21014..>21518
                     /locus_tag="PKI35_07980"
     CDS             21014..>21518
                     /locus_tag="PKI35_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HNX44356.1"
                     /translation="MKNSHFIVFLQLDSRDCGPTCLRMIAKQYGKNYSLQGLRERSFI
                     TREGVSLLGISEAAEQIGFRSMGVRIGWEQLASEATLPCIVHWNQNHFIVVYKIIRKN
                     IVFVADPAHGLVKYTQSEFLRYWLSDKQEGEEKGIALLLEPGPDFYTIEDEKPDKTNF
                     RFLFQYLR"
BASE COUNT         6019 a         5025 c         4651 g         5723 t
ORIGIN      
        1 ggagcaaaaa tattccggag tttgctccta atcaatccac cctgcaaact aaaggacact
       61 tagaacactc cggggcactt aggatctttt accacggagc cacggaatac ggagaattgc
      121 atattcttag tgaacctcag tgacctcagt gccttggtgg ttgccttctc tttaaccact
      181 aagacactca gaacactcag gggcacttag gatcttttac cacggagcca cggaatacag
      241 agaattgcat attcttagtg aaccgtggcc tcagtgcctt agtggttctt ttctctttaa
      301 ccgtcctccg ttctccgttt tccctgctcc ctgctacagc attttcttag tgaaccttgg
      361 tggcctcagt gccttagtgg ttccttcctc ttttcttact gatctcagaa atcaaaggtt
      421 atcccctgcg ccagggcggt ttgtccgctg aaattaatgg tattggtctg ccggcgcata
      481 taagctttcc aggcatccga accggactcg cgtccgcctc ccgtatcttt ttcacccccg
      541 aaagcgcccc ctatttcagc gcctgaagtc cccaggttca cgttggcgat cccgcagtcg
      601 gaaccggttt cggaaaggaa cttttctgcc tccgcaaggt tctcggtgaa gatacaggac
      661 gaaagcccct gcggaacatt attgttgagt gcgatggcat cttcaatggt tttgtacctg
      721 ataatataaa cgatgggcgc aaaggtttct tcctgaacca tcttataata gttctccgtt
      781 tcaacaacgg ccggaaccac ataattgccc gaggcgaatt tgcctccgct cagcacctga
      841 cccccaaaaa tgaccttgcc gccttccttc tgaacttctt ccagcgcacg ggtgaaattc
      901 cgtacagcct ggttatccac gagcggtccg atcagtgtgt ttttgtcgag cgggtcgcca
      961 atacggtcgg taaggctgct gtaggctgcc accaaccttt ttttcacggt ttcataaacc
     1021 ttttcctgca cgatcagcct gcgggtagaa gtacagcgtt gtccgcaggt gccgacagct
     1081 ccaaacacta ccgattgcat ggtcatatcc aggttggcat gttccgaaat aataacagca
     1141 ttgttacctc ccagttccat gatggtcttc ccgagacgct gccctacgat ggcagccatc
     1201 ctctttccta cagccgttga accggttacc gaaatgagcg gaatcctttt gtcggcagcg
     1261 aaattgtcgc ccaacacctc cgatctggtg atgatcagat taaaaactcc ttcgggaaca
     1321 ccacattcaa caagggtttc tgaaataatc ttctggacgg cgatggcgca taacggcgtt
     1381 tttgaactgg gtttccatat caccacatca ccggcgatgg ctgccagcat ggaattccat
     1441 gcccatacgg ccaccggaaa attgaatgcg gtaacaattc ctacaattcc caatgggtgg
     1501 tactggtcgt acatccggtg aaacggcctt tcggaatgca tggttgcacc gttgagcaga
     1561 cgcgactgcc cgacagcaaa ttcacaaaca tcgatcattt cctgaacctc gccgagccct
     1621 tcctcgtata tctttcccat ttccagcgaa accaggtatc ccaaatcctt tttatgcctg
     1681 cgtaaagttt ctccgatcct cctcactact tccccgcgtt tcggggcagg aacgatgtgc
     1741 cactgccggt aagcatcccc cgccttttcc atcaggtact cgtaatcttc aattgtcgca
     1801 ttgtttaccc tggctatcaa cgagccgtca tggggcgaac aggaggaagt aacatcccct
     1861 ttggtggcga gccattggct gccggttgaa gcgccgctgt tcagctcctg tattccaagt
     1921 ttattaagtt ctgttgtcat ataaaaggtt tgtgttatgg ttagtcaatc atatagggat
     1981 agcgataatc agttgcaggg acgaaggttt ccttgatggt gcgggggctg atccaccgga
     2041 tcaggttaaa ttcaccgcct gccttgtcgt tggtacccga cgcccttgat cccccgaagg
     2101 gctgcaatcc tacaatggcg cccgtgggtt tgtcgttgat gtaaaagttg ccggcggcat
     2161 actgcagttg ttcgcaaagg acggatgcta cgatccggtc acgggcaaaa acggctcccg
     2221 ttaaaccgta aggagaagtg gtgttgcata gcccgactgt ttcctgcagt ttgtcatctt
     2281 catacacgta aaccgttagt accgggccga aaatttcttc ctccatagtg cggaatttag
     2341 gatttgtagt cacgatgatg gtcggttcca caaaataccc ggtcgtttta tcgcctttac
     2401 caccggcaat aatttcagcc tctttcgaac ttttagcaaa atgaatgtat tccatgatat
     2461 tgtcgaagga attttcatcg atcactgcat tgatgaaatt catcggatcc atcacatctc
     2521 ccatgctggc atcagcgcac atcttcagca tctcggtttt taccgaaggc caaagcgaaa
     2581 caggaacata catgcgcgat gcggccgaac atttttgccc ctgaaattca aaagctcccc
     2641 tgaaagcatt taccgccact tcgagtggcg atgccgaagg atgtacaaat atgaagttct
     2701 tgccgccggt ctcgccgatc agcctcggat aggatttgta atgcgacagt tgcgaaccga
     2761 cttctttcca caggctgtta aatgtttggt ttgaaccggt aaagtgtatt ccggccaggt
     2821 cttcggactg cagcaccctc ttgccaatca gcgcgccgct gccggggaca aaattgatta
     2881 caccgtcggg caggccggct tcctgaaata ttttcatcag gtaataattc gaaagcaacg
     2941 aagtagtggc cggtttccat accgtagtat tgcccatcat gaccggagcc atgttcaggt
     3001 tcgaagcgat ggaggagaaa ttaaacggac ttacggtgaa cacaaaacct tcgagcggac
     3061 ggaattccat cttgtttaac tggttgaagg cagaacgggg ctgcatctca taaatcctgg
     3121 aagcaaaata ggcgttgtac ttcaggaaat cggtggcttc acacactaca tctatctccg
     3181 actggtaaat atttttgctt tgtcccagca tggtggctgc attcagtata tacctgtatt
     3241 tccctgtgat caggccggct gcttttaaaa ggattgaagc ccgtacagtc catgataaat
     3301 cggcccagat tttatgcgca ttgagcgcgg cctcaatggc catgtctgct tctttttccg
     3361 aagctttgta atacgttgca agtacatggg cgtgtttgtg cggcatcctt accaggcctg
     3421 tttcattggt ccgtatctcc tttcccccta ttatcaaggg gatttcaata gcctcctgca
     3481 tttgcctttt caactcggca ttcaacagca tcctttcggg actgtccttt ttgtaatcca
     3541 gaaccggttc attctgcggt tgcggaaaat tgtaaatagc gttgttcatg ttttatcagg
     3601 gatttattga tcgaattgca tgtttttgca attcggtgtg caaaagtatt attttccggt
     3661 cactatagaa aaagagggga attttatttt atttgatcct tcaggtcgtt catgatcctg
     3721 tcaaaacctt gctgaataat aagatcaaat tcagaatgga atttttcgaa cgcctggaca
     3781 ataattttac cttcaactgt aaggatggta ttgccgcctt cggcgccgcc gcgtgtgggt
     3841 tttatcagcg ggaaacccag ttcgcgttca attttatgaa ggttatccca ggttttccgg
     3901 taggtaaggt taagcttttt catggcttcg ttgagcgaac cggtttcttc gatcgctttc
     3961 agcaattcac attttttatc tcctaaaatc ccctgcctgt ccgaggtttc aagccatatt
     4021 ttataattca gtcgcagatt atccaaacgg gagctcatat gagtggcttt tattgtcctt
     4081 ttgctgatga caaacttaaa gaatatacgg gtttgatcgg aagcttcgcc gggctacctt
     4141 ataccactaa ggcacttaga acacatagtg gcactgagga tcttttacca cggagccacg
     4201 gaccacgaag aaaaacagag aattgtatat tttcnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnttagtg aacctaagtg ccctatgtgt ctaagtggtt cttatttacc
     4381 acggagccac ggaccacgaa gaaaaacaga gaattgtata ttttcttagt gaacctaagt
     4441 gccctatgtg tctaagtggt tcttatttac cacggagcca tggatcacga agggaacaca
     4501 gagaattgta cattttctta gtgaacctat gtgcccctaa gtgtcttagt ggttcacctc
     4561 tatttaacaa caaccaggct gaccggcagc gtgccgctct tgccaactgc agctataaaa
     4621 cggacttccg tctgcgaaaa atcaccggta atttccaaat tccctccggt aatttccgat
     4681 tgcaccacgg gtgcaaacag tactttattg tccattaaaa cggcaatggc tttgccaagg
     4741 ttgctttttg ttatatcagc ccaaaccccg ataacaggtt ccctgaatcg gaaaccgatg
     4801 gattgccacc gggcctgagc gtcctgcttt gtctcgaaac tttcaatgtc ggcgcccgtg
     4861 agcggaatga tgttttcttt ccccgtctgc agggcataca ggcaggcatc ctgctcatca
     4921 aacaggttgc tccaggcaaa cctgacttct tcgttaaccg aagaaggggc aaggtaccgc
     4981 tcaaaataac cgatactgtc ggccgaagtg cataacagcg tagcggaaga tgcagcattc
     5041 ccccgttcaa gcagcatatt caccaggttc ttgtcatcct tcacaagatc tttaaggctt
     5101 tgattattat atgttttata aaacgaaaac atccctttct gtgtgatcag gctttttatc
     5161 attttcaact ccgcgttgcc gtccacagtg atccggatct ggttacggcc ggaaacggcc
     5221 ttaacatcaa aaggtccgga aaggaaattc ttcagccgca gcgtaatgac ttcggcagcc
     5281 ttattcatct gatccggaga aatgttgctt tcggctgatt gcagaaaaat tacctgtttt
     5341 ttttccggca cggtaacgaa gccggttgca atgatcccgc aaaggaaaat tgcgattagt
     5401 gcatatttaa tcgatttcat tgtacatgaa ttataagtta aaaacgaggc cgcttccggg
     5461 cggaagggcg aaaaaataat ccggtgcgtt tttaacttaa aaagggcagg tcatccgcct
     5521 ctatagtgtg atactgataa taaaaccgtt gaagaatgac aggtttgatc aaacgttcgg
     5581 ctttttcatg gaaccggatg ctgtgttgca gaagcctgtt ttcggccgcc gttttaaaac
     5641 tattgccgaa cgtcttgaaa ttcttcctgt catatacagg aatactgtta ttcaggcagc
     5701 ggaactccag ggtgtaatta accgcaacaa gctgatattg tgtacaatat tccgaccgta
     5761 actgaccggc attctcaatc cggttatcag gcttattgga aaatacaagg acaaaaagaa
     5821 agaacagaag cacgataact gcgcttccga aaaatatttc cctgtttccc tcttgtctgg
     5881 tcatggcaca aatatagaca ttgaaatcga aatgtgaaca tgaattgtga tttaatgaca
     5941 ttccggccgg acacaggtgt ttttatgatt gttattttca acctttgtag cggtctgcat
     6001 tcctgtttcc tctgatgttc ctttgaaaaa aacaaagaag aattccattc ctgaaatttt
     6061 ttttatcccg gaacctgcaa atcctgaatg taacttcatg aaacatgatc ctgttcaggc
     6121 aaattgccag aaatgattaa tgattttatc ctgcctgaaa cccgacatac aggttaaaat
     6181 gttgtgctgc aatttttaat gacggccaat ttaaaatgaa tattaaccaa ttcgcggaaa
     6241 cgaactgttt gcagacaaaa ttcatgtgca aaggttataa aaatcacttt aaaaatacga
     6301 tcaatattaa gcaagagtaa ggattcaata tagaacagtt ataaattatt tatgggctaa
     6361 ttacattcga aaagatggtt tatgaataat taaaaggggg caaataaccc gtcttctgcg
     6421 gataaggggg aaaacttacc gcacaatgtt aaggaaaatt tctgcaaacg aaaagagctt
     6481 gtctctttcc gggcaggata attatacctc atgaaaatga agttaaaagg caggataata
     6541 acgtcaagcc gttttatccc gggttcccgg gaaaaaataa cacccgatac cgctgcaaac
     6601 cggggtggat ccccgaaacc agccggcaaa cacgccctct gtgcttgcgg gggcgatgct
     6661 gtgcgcaact gttttatgaa tcctatcatc catcaataaa cagaaaatta acacctgaac
     6721 atatgcaaga aaaaaacaag ccgccctgat cactcatctg aaaccatgac ccgatacttc
     6781 tttgccccgt gtaaggaaat ttctctcatc aatactcact aaaactcaat tcactcaaat
     6841 taatccaaaa caaaaaaaat gaaaaaactc ttctttgtta ctgctatcat gctgggtttc
     6901 gcagccggcg ctttcgcaca ggtttcggct acggctagcg ccgaggctac catcgttact
     6961 cctatcgcga ttaccaaaac cgtcgatatg aatttcggta atgtggccgt taatagcacc
     7021 gcaggaaccg tcgtattggc cactgatggt tccaggacta ctacaggcgg cgttacctta
     7081 cctgtaaccg ccggttcacc agctgcggct tcattcacag taaccggtac agcgggttat
     7141 acctatgcca tcacgttgcc aggtgcaact accattgcca acggtgtaat caccatgggt
     7201 gttgatacat ggaccagttc acccgatgct accggcacac tcactggcgg cactgaaaca
     7261 cttaccgtcg gtgcaacact gcatgttggt gctgcccagg ctgcaggagt atattctaac
     7321 gctactgctt gccctgtaac cgtaaactac aactaacatc aacaaagtga atgaggctgc
     7381 ccctcaacgg caaagcctcc gcgggcagcc tcattgcttt ctattttcaa ccgataaata
     7441 tccattcccg acgatgaccc gttttaccct ttttaaaaga tttaaagcct ctttgtacga
     7501 aaaacggatg gttctgtttt tggccggaat atcagcagtt gccgttttta caacttcttc
     7561 agcctttgca caaggaaacc tgatccttct ccagaaaaga gtggtttttg aaggaggcct
     7621 gaaaaacgcg actatcgaat tcgggaacat cggcaaggac acggcccggt atgttgtttc
     7681 gatgcttaac atgcggatga aagaggacgg ctcattcgag aacatcacgg agccggattc
     7741 gggacagtac tttgccgaga aatacgtacg ctttttcccg cgcaacatcg tgcttgcgcc
     7801 aaatgaatcg cagacgatga agatacaact ggtgaacacc cgcgatttac aaccgggcga
     7861 ataccggtcg cacctttatt tcagggccgt acccaacatg caggataaac aggaagcccc
     7921 tgccggaaac aaagagggta tatccatcag cctgaaaccg gtcttcggga tcaccatccc
     7981 ggtaatcatc cgggcgggcg aaccaaatac gacggttaag ctcagcgatc ttgccctggt
     8041 acagcaaaat gattcaacac aggccgtaaa ttttaccatc caccgttccg ggaatttctc
     8101 ggtttacggc gatataacgg ctacttatat agcagccgac ggaaaagaaa ttgaagctgg
     8161 cagcgtaaaa ggcgttgccg tatatacacc gggcaccctg cggaccagtc gtctggccct
     8221 cagaaacatt gacggagtgg actatcacaa tggaaaactg gtagtaaaat acgccagaca
     8281 ggctgaagaa gatgacgctg taatggctga agctgaactg aaactgaaat aaggaatcaa
     8341 tggttaacag tataatacag gcaatgggtc ataaaacgca cggcaaagaa attcaacgaa
     8401 tgaaaaccat caccggaaag atatatttat ttaccctcct cataaccctc accgcttcgc
     8461 tgtatggtta cagtcagggt gttgccataa atgccgacgg atccgcaccc gacgcttccg
     8521 ccgctctcga tatcaattcg gccaataagg gtttgctcat cccccgcgta gcgttgacct
     8581 ccacttcttc caatgagccg gtcggctccg gcattgcaac cagtttaatg gtatacaaca
     8641 ctgccacggt caacgacgta attcccggtt actattactg ggacggaaat tcgtggatac
     8701 agtttaatgc accggaaacg ggatcacagt ccctgaatat tgtatataaa acttccagcg
     8761 gaccaatcgc caagactgag acaatggtga tagctaccgg agatattacc ctcaccctgc
     8821 cggcagtgac gagcgccgat aacggattgc agatcactat attaaacgcc ggaacctaca
     8881 aagatctcgt agtggtaaaa ggtgcagcgc cgggcgtaac catacattcg gtggattcag
     8941 tgctcttcta tcgcgggtat tccaaaacgc ttgtagctgt taatggtatc tgggctaaaa
     9001 aggagcacat ggccctgcag gacaatgtct ttgaaatttc aaataaatcg agctggaata
     9061 ctatcggcga agccgttgca ttcctgaaag agcacatgac aggcccttcg gtcatccggc
     9121 tgggaggcgg cacttatgaa attgatacta cccttactat caaccttcca tacccggtga
     9181 ctattgaagg ggtatcttac ggagaggcaa tcattcaggg atctgccggc ttatcgggtc
     9241 atgaaatgtt taattgccag agcgaatgct actttaagat gatcaccttt caagcggttt
     9301 ccaacacttc cggaaatgat ggcatccatc tgacgggcag cggggagtat tacgaaataa
     9361 aggactgtga atttgacggt ttttataagg gaatcgccgt taccagaaac tgtgaaatgt
     9421 ggttgtttga aaccgacttt tatgacatta ccagcatagc cgtttctgtt gaagatgcca
     9481 gcggcagttc cgacgggccg gttattaaaa tgtccgaaac cgattacaca aattgcggta
     9541 cagggatcaa gctggtcaaa gggaaaaacg gtattgtcga tatcgtgaac tgtacttttt
     9601 ataacagcaa cgcaggacaa acaggaatcc tctatgttcc cggtctcgga aattttcaaa
     9661 actttacttc catgttcatc acgaataatt cgtggaacaa tatcggaact ttcatgagcg
     9721 gtttcgactt tacgcgagcc gacggcaggg atgtcaacgc ttttatcgag aataacgcag
     9781 gcatggggaa taaaaacccc tatgttaaag taaatgttat cagtaacaca gccagtaccg
     9841 atcttcctga ctcccgttat tattatcagg ccaattttgt taatacttcc tctttccctt
     9901 gtaaatggaa ggtagaaaac aacaaaatca cctatctgcc ggcaaataaa aaagatgttg
     9961 tcatgactgt tgccggtaat attgccggca ccagcggcag ttatattacg atcgcggtgg
    10021 ggaaaaacgg acaaacagtc tatgcgccca ttaccatacg atccagaaca aacggaacat
    10081 attttgcctt taccacggtc gcttatatcg agaatgtttc tcaaggcgat tattttgaaa
    10141 tttatattaa ttcgagtagc agttttgatg atgctatcct ttcggatttg aactggtggg
    10201 ttgataccaa ataacgggag cagatagatg ggacagccga tgaaaatccg gatcaaatta
    10261 ttcttactgg tttataaact tctcctgatt ccatgtttgg tttcaggaca gggaatggtc
    10321 atttcctccg gaagccatgt ggtagttgga accggttatg tcggcatctt cggcaacctt
    10381 accaacaacg gttcctacac cgaccagggc ggcacgctgg ttctcaccgg aaacacacaa
    10441 aaccttaccg ggacaaacgg cgccacgctt aataacctga acgtatcatc cggttccact
    10501 tccacgctct caacggcagg acaaaccgtg aaaggggttc ttacctgcga cggcacgtta
    10561 aatgccggcg gcaatcttac gctgctttcc acggctgacc aaacagcgct ggtgagcggg
    10621 gccggcgccg gcaccatcaa cgggaatgta acaatgcagc gttacctggc atcaggtttc
    10681 gggtataaat atttcagttc acccttccag gcggccacgg tcagcgaatt cgccgacgac
    10741 atgaaccttg gggcagcctt ccctaccttt tacagttaca acgaaaaccg ggacacgacc
    10801 tggtggataa cctacaccac gggtaccaac ctgcttgttc ccatgctggg ctatgccgtg
    10861 aacttcggcc cttccgccga tcccaaaacg gcagatgtca caggagcggt atccaacgga
    10921 actatgtcgc tgactttata caaccacgat aaaaaccata ccaaaggatt cacgctggtt
    10981 ggaaacccct acccatcccc tgtcgactgg gacgcagcca ccggttggac aaagaccaac
    11041 atcgacaatg ccgtttacta tttcaacgcg ggaagcgccg atcaatacgt ggggagctat
    11101 agtacttata tcaacggcgt ttccagcgac ggcatcgcca acaacatcat cccggccatg
    11161 cagggttttt ttgtccatgt aaccaacggc acattcccgg taacaggaac tctcggattt
    11221 tccaatcagg tcaggatcaa cacgctgaac cccgtttttc acaaaaatgg ccttcttgat
    11281 tacaggccgc ttatcaggat atcggcaggc tttgaaggtg ccgaaaaccg tctcgacccg
    11341 ctggttgtcc tgttcgacgg ggaggcatcg ccgctgttta acgaaaggca ggacgccgta
    11401 aaattgatga ataccgatcc cgaaattccc agcttttata ccctctctaa cgacaatatc
    11461 cggtatgcca tcaaatcgct gcccgagccg gccgattcgc tgacagttat tccccttgga
    11521 attaccacac aacagcaagg catcattgat tttgctgtaa acgccctgga aaaccagcag
    11581 cccggtttgc agctttattt ttccgacaat aaaaacaggg ccgtccttgc cgtgaacagc
    11641 aatacccgct atcaggcgtt tttggacgaa ggtgtatgcg acggacggtt ttcgctcatt
    11701 ctttcccgcc atgaaattcc gaccagctcc ggctcagccg atgaactgga cgcctattac
    11761 agcgacggca aaatatatgt ttactgtgat tttttaaaca acgaacaggg tgaactgcag
    11821 gtttcggata tgctgggacg cattatccac cgggagaaag tgaccggcat cggatataac
    11881 agtgtaaacc ttaatgtacc gaccggcatt tatatagcca gcttaagatc ggaacacgct
    11941 tcttcctcaa agaaactctt tatccccgga aaatgaaaca ccgctgcctg aaattattat
    12001 ccctgttgcc atcgctgaaa ggcgccatca tgcttatcat gatcgttagc ccggtgatcc
    12061 tgatgggaca ggtaaatcct cccaggccga taacgctgac tgtatcaccc gtgcagaacc
    12121 tgaatttcgg cagtttttgc atcacgggcg ccaacggcgg cacggtaacc atttcgaata
    12181 cgggcgtacg cacctcttcc ggcgatgtct tcctggttaa ttcaggatat accgaaggca
    12241 ttttcaccat cgaagtcgcg accggcacat cgctggtgat gatgaacggg gctgacgcca
    12301 cgcttaccgg cagcaacggc ggaaccatga cactgaccct ggggcagacc gatcctccgc
    12361 ttccttacgg cacaattacg ccgtccacag tctttcatat aggcgccacg ctcaacgtcg
    12421 ggcccgtatc aaacaatccc ccgggatcat actcgggaac ctatgagatc atcattaacc
    12481 acgaataata aaacctgaac gatcagcgaa cggatgaaga aaacgtattt aaaagagaga
    12541 accggcagga tcctgcgttt cgcagcagga ctcattgtct gtgctctctc cgttttcgcc
    12601 gcgccatccg tttttgccga caataccgat gtggagccgg cattcgatga ggtttcgctc
    12661 acgctaaaag tccataaatc ggctaccgtc gaaattcccg ctgtgatcag cggttctacg
    12721 gcatatttgt cggtttccga cctgtttact ttcctgaaaa ttaagaatat cgtctcggat
    12781 gaatccaatg taatatccgg aacattcctg aatgaatccg atccatatct gatcgataaa
    12841 acaaggaata tcatccgcta cagggacaag gaatacctgc ttacccataa cgagctgatc
    12901 agaagtgaat cgggtttgtt tttggctacc aacctgtttc aggagatctt tgggctcgaa
    12961 tgtactttct atttcaggtc tctttccgtc gaccttgatt caaaacagga acttcccctg
    13021 ttccgggaaa tgcggcagga agagatgcgg aaatacatcc gcaaggtgaa aggcgaaatt
    13081 catgccgata cgcttatcgg acgccaacgt cccatgctat atgccggaac ggccgattat
    13141 gccgtttatt caacgcaggc aaccgacgga ccggccacca cgagggccag cctgtcggta
    13201 ggaggattcc tggccggcgg agaaaccacg ctgggcctca actattacac cgatcagcct
    13261 gtcaccgggc gaaaccaata cttccgctgg cgttatgtaa acaacgataa caaagcattg
    13321 cggcaggtcg ttgccgggaa tatttccaca ccgtcaatag ccagcatttt ctcgcccgtg
    13381 atcggggtcc aactgaccaa cacgccgaca acctaccgga aattttacgg cagctatacg
    13441 ctgaacgaca ggacagagcc cggatggatc gttgaactgt atgtcaacaa caccctggtt
    13501 gattatgcca aagccgacgc ttccggcttc ttcactttcc aggtgccgtt ggcttacggt
    13561 aacaccgaag taaaacttcg cttttacggg ccctgggggg aggaaaaatc gaaggttcag
    13621 aacatcgtga tcccctttac cttcctgccg aagaatgaac tcgaatatac ggtaagcgcc
    13681 ggcatggtgg aggacagcac gaacgccctg ttctggagga gcagcgtcaa ttacggtatt
    13741 tccaataaaa taagcattgg cggcggcaac gaatatcttt ccacgattac caccggcagg
    13801 aacatccctt acctgaatac ttccttcagc cttaccggca acctgttatg caacgctgaa
    13861 tacgatttcg gcgtcaggaa aaaaggcctg gtttcctacc ggctgccttc caattttatc
    13921 gtggaacttt cctatacgaa atacgataaa gaccagaaag ccattttcta taattacctg
    13981 gaagaaaaaa aaggaagcat ttcgctgccg ctccgcttca ggaatttttc catttattcc
    14041 cggttgtcgg ttaaccacgt atcgctgaag gaatccatga aactgacctt gtcggaattc
    14101 atcgtttcgg cctcctataa aaagttcagc ggcaatatcc atagttacgg ctattttttc
    14161 aataccggca ccccggtaat gtataccgac ctttccctgg gctacaggtt gacgaaagga
    14221 ataagcatca ccccaacggt gaggtacaac catacccgga accaggtgac aggtttgaaa
    14281 tgcatcgccg agaaacagtt gtggggccgc ggctacatca ccatgaccta tgaacagtac
    14341 accgattaca acatcaggaa tttacaggtc ggcatccgtt atgttttctc aaaggcccag
    14401 gcggcagtat atgcaaacca tcataacggt tctgtcagct tttcggaagc cgtgagcgga
    14461 agcctggtgt ttgatccggc aacatcctat gtaaaggccg gcaacatcaa caaagtcggc
    14521 cggggtggca ttaccgtgct gcctttcctc gatatcaact gcaacggcag gcgcgataag
    14581 ggagaacccc gggtaagcgg gctgaatgta agggtgataa ccggcggacg cgttgaggtg
    14641 tcggaaaaag attccctgat ccggattttt gaccttgaag cctataccaa cgcatatctc
    14701 gaaatggacg gcgccggcct cgggagtatt tcatggcaat tgtataaaaa gacatacagc
    14761 gtaccggtag aacccaacct gatgaaactc gtggaagtgc ccgtaaccgt ggtcagtgaa
    14821 gtttccggcc tggttaaaat gaaaacctcg ggacaggcaa tccggcggga aaatattaaa
    14881 ataaacatct accgggacga caaggtatta gtgacttctt gcttccctga agacgatggt
    14941 tactacaatt atatgggcct gacgcccggc aattacacca tcatcccgga taccgcccgg
    15001 ttgagcggca tgcagatgag tgtcgttccg gcatcccgga aactttcaat acctgaaagc
    15061 cgcgaggggg aagtgttcga caccctcgat ttcatcgttg agtcaatgaa acccgctgcc
    15121 ggcgaagtaa tcccgaatcc ggctgaatat accccgacag tggcagccgc cgcaccggat
    15181 acaacaacgc gccggcctgg gaaaaccggt cctgaacaga ttattattac tgatacttcc
    15241 gcgttaaata acacagcgaa ggccgccgtc acaacaaaac cggaaacgcc ggctccggca
    15301 atagaggatt taacccgtgt tgcttcagat ccggcatcag ccatcagcgg ttccgaaatt
    15361 gacgatctcc ccggttacta cgttgtgcag gccggcgcct tcaaaaaaca attcaatgct
    15421 aaaaatgtaa aaaaacatat tgcttcgcag tacaatgaga aggaagtagc gattgtgagc
    15481 gcacagggct attacaaggt gcttgtggcc ggctataaca gcaaggccga agcgatggcc
    15541 atgcggaatt cgttaaggga aaacggttac ccggaagcct tcatccggaa gttgtcggga
    15601 ggaattgcca tccgggcctt cggggatctc aacagcaatg gtatattcga cccgggcgaa
    15661 ccctgcgtga acggattaac agccagcctt tctaccggtg gaatccttga agtgagcccc
    15721 gccgataccc tgatccgtat cttcagtttt gaagccgaca gcggcgccat tcttgaagtg
    15781 gacttcagcg ccaatgaaag ccatggttgg gagctgcgac gtaaaacctt taccgtggaa
    15841 accgacccgg tacggatgaa actgctggaa atcccggttt atgctcctgg catgatttcg
    15901 ggtacggtaa ctgccgaagg gccggccggc gcgaacatgc ccgaaatgat gagaattcag
    15961 atttaccgtg ccgacagtac cttgttaact acccttgatg ttccggaaaa cgggcgattc
    16021 agctttaaag ggctgaaacc aggccattac cggatacagc ccgataccct ccaattgcaa
    16081 aagctccggt tgacaagcat gccggcaggc agggaaatca agctgaccga aggccgtttc
    16141 ggcgaatcag ttaccggcgt tgattttgcg ctggcgccgg aagtacagtc ccgggtaatt
    16201 cctctccagc ccaatgttta tcctgaactt gccgtcagcg attcggcgct gctcataaaa
    16261 ccggaccgta aaaatgagat tgtcaacgga tgtttcgcta tccagttggg cgctttccag
    16321 gaacattcca atgccaggtc tgtctcaaaa cgtgtttcct tcaggtatca ccttaaagtt
    16381 gatatcatcc agacagcggg atactacaag gtgctgatac ccggatatta cagccggctg
    16441 gatgcaacca tgatgctgaa taaattaaga aagaacggtt ttccgggggc atacctaatt
    16501 tatattcccg accggaccgg ttcggaacag ccgtaaacag aaccggacag ccttcgtttt
    16561 cccccgtgca ggcgcttccc tgatgagcgt gcaaaacctg tcaggaagcc ctgcattccc
    16621 ctttgttttt gacttcttcc ccctgttttc atttttcttc cggaatggcg tcgggttacg
    16681 atgcagccct atcggcgcgc gcatctcccg gcacgggtga ccataaaaac catgctgccc
    16741 ggtggaggat aaaaccgaag ccagaccttt gtctttatta tctgtatcaa cacggtttac
    16801 aggattcccg ggatgaccgt gggaacccgg agaacagaaa taatcatgcc cggtacgact
    16861 tttaattatt tagtaattta taaattgttc aaatttttcc aaagcaatat aaaaacctat
    16921 agatttcttt tctaatttag aatgctgata tgcaggccac ccgcggaacc ggagaaacgg
    16981 aggcggcaag gggaagtgtg aaccaccgga atggttaaaa gtggtaagtt tttgatgata
    17041 tattgtaatt agcaacaagg cgtagaaaaa accgaacacc cacccacagc aaaagagttg
    17101 caacacattt gctcttggct gtcacacaag gaaaatttgg aaagctgaca gaatttgtaa
    17161 gacagataca atccagtaca aaatttttaa aactttacat tatgcgtttt atcgataaaa
    17221 aacagaaact cgattatttg attgaattga tcgaaaaaga agacactgga accgctgata
    17281 aactatgctc aaaaatttgt gtatccaaat caacgttgaa acgctgctta aacgacttac
    17341 gcgaatcggg ttatcaagtc gtatttcatc caatccgaaa cacatattgt tttattaaca
    17401 agccttctca acaaaaatat gggtgatgtc cataaatgag cctgcaggtt cagtttttga
    17461 gcctgccata gatttacctt tatacaaatt ttaagtatca tgactcatga ctattcaaaa
    17521 accagataac acttcacttg ctgtttcatt cttattaagt aagtttggta taaaattcaa
    17581 cgataactat ctaataaaag aattacgatc tcatcctttt tacccaagtc ttgccgcaat
    17641 tagtgatttt ctccaaaaat ataaaattga aaatgaggcc cttagattat gacgagttat
    17701 tacagtttaa aaatcctttt atagctcatc taactatttt taacggaata tttgttgtag
    17761 tgcttcaagt caatgaccat gaagtaaatt attttttaga aggcaaaaat tatgtaactc
    17821 tatcaaaaga agaattccta aaagtttgga acaacattgt acttatagct tatccttcag
    17881 cgcaaagttg tgagcctgaa tatagacaac accatcagga gtatcttttt aaaaaggtta
    17941 aattgccatt attgttgagt gttttaggac ttattcttat catacaattt gtttccaatt
    18001 tttcagcaat gcaactattg cctcttttgg ccacaaaagc aataggattg ttttttgttt
    18061 cgttgttaat caaacatgaa ttgggtattc acagcgaaat ggttgataaa ctatgtacaa
    18121 tgagcaaaag tgcagggtgt aacgaagttt tgaaatcaaa aggggcaaaa ttatttggaa
    18181 aagtactttt agctgacctg ggtttagtct ggtttgtctc atcctttatt tttatcttta
    18241 tccatctctt tacatccaag actgattatg agaatatctt taatctgttg ggttggctgt
    18301 caatagcatc agtaccattt atcttgtttt cagtatctta ccaggcattc attattagaa
    18361 agtattgtcc tttatgcctt ggggttatga gtactttaat tgttgatgta ctattgctta
    18421 ttgccatgta tggattccgt tttgcaattc cttcatattc gtgtatttta ttaatgataa
    18481 gcatcttatc ggttgtgttg tacgtttggc ttttaataaa gaactattac acccgagcaa
    18541 atacattgga tgatattgaa aacaaatacc tgcatctgaa gcgcaatcca agtgttctcc
    18601 aattagaact gcaacatagt gaaaatattg agggcagcaa tttcccaaat cctttacgat
    18661 taaccgagaa taattctgaa attttactta ctgaagttat aaacctctat tgctcaccat
    18721 gcaaacaagt tattgagaag attgacaagt tattgcaaaa ttgtaacggc aactgtcctg
    18781 atttacaaat agttctttta gcgaatgcaa aggataaagc cgatgtaatg acgataaccg
    18841 ctgcccattt gttatctatt gctgattatt tgacacctaa ccagcgtttt atagcattgc
    18901 gagaatggtt agagagtatg gattataata aatggtcaca aaaataccct gttgaaattt
    18961 ctgatcgaca atttgccatg ctaaaagaaa atgtagcatg gttcaaggaa cacaaagttc
    19021 atggcactcc aactgcgttt ctaaacacaa aaaaaattcc tgataatttc gacataactg
    19081 acatacaata catgattaac taaactaata ttactatgca cgatacctaa caaaaaccga
    19141 agcgaacatc cggacatgga tgtcgcttcg gggtctggct cacctaccaa acaggtgaaa
    19201 ccgacaatta aacatttaca aaattactaa agtttttgct gctggttgat gcaaaaagca
    19261 gtccatttta tgaaactaaa taaagatgaa atgaaaaaag tgcttggggg aactccagga
    19321 ggaggaggta gtacatgtaa aatcacctgt aaaaataatg atacaaatca aatatatgga
    19381 actctagaag tttcaaaatg caacgacaat gatttaacta aatgtataaa cgcttatcca
    19441 gatacaactg ttgcagtttg tggttgttct ggggatcccc aatcataaaa accaaattgt
    19501 gtctttctgt agaattttta aataaaaaaa accacatggg attcttagaa ttcactttaa
    19561 gaatcccatg tttaaaaaaa acgatgaaat tatatatcca actccttgca attctaatat
    19621 ctttaagctg caatattaat tgttgtcttt gtcaatcgaa tacaacaata tctggaataa
    19681 tacaaaacgg ggaaaatcgg ttcatagatt tatcttgtga tgatttcctt ttttttgaaa
    19741 gacaagaaat tgcaaaaacg aaaattgatg ataagggaaa cttttctttt aattttaata
    19801 ttgcacagcc aatgaaagtt agactattta ataaattatt ttatatctgt cctggagata
    19861 gcgtcaattt ttatattgaa ggcgacatgt atgatccatt aaaaatccat gtttctggca
    19921 ccaattcaaa tccttataat tgcttgattt tgcttgatag tttatcaaaa tccttgccat
    19981 ttaaacccta tttttataat tttaaactcg gactttccaa ttatttagat tctattaaca
    20041 tcaataagaa agcgatgtta gctttattaa gtcaatattc cgatgaattc tatcttcctt
    20101 ctgattttct tgcttatgcg tcaagcaaaa tcgtttatga tttttattat caactgatgt
    20161 ttccaattat aagtagaaat ttcccaatcg atcaaatacc cctcgcctac cttaacctat
    20221 taaatgaaat tagcctgaat tatgacagtt tggcaaaaga tagagcgtat gcgtttgctg
    20281 caagtcaatt tataaggttc aaagaaaaat caactacata taactcgttt gatataatta
    20341 atatgcactc aaccggactt acaaaagaat tattattaac agatcatgct ttttcgcttg
    20401 ttcaaaatta ttcttcaaaa gacagtactc agatttctct taccatccaa actattgata
    20461 aagaaataaa ggataaagag tttcgcaaat actttagtat tgctaaggag aaattaggga
    20521 aatatttaca tcctatacca gaggaagttt tgaaaactgt actgatagat agttcaggta
    20581 atctactcac atttaatgat ttgcttgata aatcctctaa taaaattatc gttttagatt
    20641 tttgggctag ttggtgtggg ccatgtattc atggaatgcc attgtttaac aagctaagtc
    20701 tgaactacgg caaatctgat atgttgtttg tttttctatc acttgataaa tctgatagtg
    20761 attggagagg aggtttaagt aagacccaaa ttccagggaa ccattatcat gtgttaaata
    20821 attttaaatc tgatttagcg aaattcttag atatcagaac tattccccgc tatgttatac
    20881 ttaatagaca gcacatgctg gagaagcttg aagctcggga cccttctgaa ggcaatggat
    20941 tatcgaaaca aattgacaat ttattactta attaattact cttttcattg gaaaatctat
    21001 agtgaaaaac agtatgaaga attcacattt cattgttttt ctccaacttg actcccgcga
    21061 ctgcggtccc acctgccttc gcatgatagc gaagcaatat ggtaagaatt acagtttgca
    21121 agggttacgt gagagaagct ttattaccag agaaggtgta tctttattag gtatcagtga
    21181 agcagccgag caaattggct ttcgcagcat gggagtgaga ataggctggg aacaattggc
    21241 atccgaagca acactgccat gcattgtcca ttggaatcaa aatcacttta tcgtggttta
    21301 taaaattatc cgaaaaaata tagtttttgt agctgatcca gcccacggcc tggttaaata
    21361 tacacaatcc gagtttctcc gatattggct aagcgacaaa caagaaggag aagaaaaagg
    21421 cattgcatta ttattggagc ctggacctga cttttatacc attgaagacg aaaaaccgga
    21481 taaaacaaat ttccggtttt tgtttcaata cctgcgcc
//
LOCUS       DAOPYV010000053        21272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_2542_length_21272_cov_12.142480, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000053 DAOPYV010000000
VERSION     DAOPYV010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21272
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2542_length_21272_cov_12.142480"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..2633
                     /locus_tag="PKI35_07985"
     CDS             270..2633
                     /locus_tag="PKI35_07985"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX44357.1"
                     /translation="MKKELIAVFIILFILSSAHAQRVSVSGYLKDAASGESLIGANVM
                     VDSTITGTTTNHYGFYSLSLLPGSHVLKFSYIGYQPQKIIVGVGRDTLISVMMEPGIA
                     MKGIEVKGDAVNASTRSSRMGTVELRLTQIKKLPSLLGEADLLKAVQLLPGVQSGNEG
                     TSGFYVRGGGPDQNLILLDGVPVYNANHLFGFFSVFNNDAINHAELVKGGFPARYGGR
                     LSSVLDISMKEGDMKKLKVTGTLGLIASKLTIEGPVIKDKTSFIISGRRTYLDYIAQP
                     LMQMESQRYSTESGYYFSDLNAKVNHIFSTRDRVFLSVYSGNDKFYEIQDTRDYLYEG
                     QIYKESGQSKLGWGNLTSALRWTHQYSGKLFGNAMVTYSNYKFYVKQDAMNFEISDTG
                     AVQSQYNLDYYSGIRDISAKYDLDYIPHPDHYIKSGICFTRHIFRPGASVTSSKIGTG
                     QETDEKAGTEDIPVNEVTYYAEDDYRITEKLKINGGMHFSGFILPEKSYFSAQPRISA
                     RYLLTPAVSLKASYSYMSQYIHLLTNSNIGLPTDLWVPATASVPPMKSSQWSLGLSVD
                     LPNDFQFTFETYYKTMKNVIEYKEGASFMSSYTNWEEKVEPGKGWGYGTEFFLQKKTG
                     ALTGWIGYTWAHSDRKFPTINDGKVFPYKFDRRHDVSLVLNYQIDPHWDVSVTWVYGT
                     GTAITMPTVEYMVINDMYGLYTVQSYSERNSYRTAPYHRLDLGFNHVKEKKWGERTWS
                     FGLYNAYNRKNPFYYQIVRDYSEKKALQRVALFPIIPSVSYTFKIGK"
     gene            2642..3571
                     /locus_tag="PKI35_07990"
     CDS             2642..3571
                     /locus_tag="PKI35_07990"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HNX44358.1"
                     /translation="MRLTYLLTAMMLLVAGCTEYESIEDFPIAEPALVANSLFQADSA
                     ITVYVSRSLSILDNAEIKPITDATVVIYKDGLAVDTIRESNESQAYVSAIFPESGHRY
                     SVRVFRSGYDTVTSTSESLPAVLSVGKITAVPVDSTITELWYQDNVHDDSAFTYYSMD
                     VALTINDPVNEHNIYSVMVLKEDSSIFSSDLRYQTSSVYFTSNDMSLLDNADLENSGS
                     YRTVAYFDDRNFDGKQYQFKFRIEDNEWTTFAAKYRVLVRSLTETSYKYFRSLNSNYN
                     ETSPFDEPARVYTNIHNGFGIFAGYERKSYRIR"
     gene            complement(3580..4740)
                     /locus_tag="PKI35_07995"
     CDS             complement(3580..4740)
                     /locus_tag="PKI35_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX44359.1"
                     /translation="MARILVIDDERAIRNTLREILEYENYQVDDAVDGPSGIELVSKE
                     TFDVILCDIKMPQMDGIEVLEKVQQITDAPMVMISGHGTIETAVEAIKKGAYDFIAKP
                     LDLNRLLITIRNAMDKSRLVTETKALKRKVSRQYDMIGESPAIMKIKDMIERVAPTDA
                     RVLITGDNGTGKELVARWLHEKSNRADGPFVEVNCAAIPSELIESVLFGHEKGSFTSA
                     IKQRKGDFEQAHNGTIFLDEIGDMSLSAQAKVLRALQENKIMRVGGEKEIPVNVRVIA
                     ATNKNMQEEIAVKNFREDLYHRLSVILIHVPALNERTEDIPLLAEHFMQLIAESQGKT
                     PSVFKPEALDELKKIHYTGNIRELRNIVERLAILCGQEITAEDVKTFAQPFR"
     gene            complement(5001..6413)
                     /locus_tag="PKI35_08000"
     CDS             complement(5001..6413)
                     /locus_tag="PKI35_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX44360.1"
                     /translation="MNKIFLIIRREYLTRVRKRSFIIMTILGPVLMAALFIVPIYFAT
                     MQDEKRIVHVVDETGLFISKFVNSDNYNFIPQYTDLETAKKTLKQTEGYALLYIPRTE
                     MNVPSQAMLYSDKQPSLNLKSYITGIMNREIEKQKLAAEIRNQIRISTPGYEARADSA
                     ADNLMSETILKNIKTDVKLLTYQIEEGGTEKKSFAEVNMIVGMVASIMIYLFIFMFGS
                     QLMRGVIEEKTSRIVEVIVSSVKPFQLMAGKIIGVALVGLTQFVLWIVFTAILVGGFQ
                     QAFPEKFKMPEAQQKFVTTQQIQPENPGEDQSANLLADTGNETANQIFEAIASINFPL
                     MILSFLFFFIGGYLLYGAMFAAIGSAVDNEADTQQFMLPVTVPLILAIVMMQYVINNP
                     EGSLSWWLSMIPFTSPVIMMIRIPFGVPYGQLALSAALLIAGFIATTWLAAKIYRTGI
                     LMYGKKVNYGELWKWIRYKG"
     gene            complement(6440..7387)
                     /locus_tag="PKI35_08005"
     CDS             complement(6440..7387)
                     /locus_tag="PKI35_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX44361.1"
                     /translation="MAILTVNSVSKHFATQTALNKVSIEVPEQCIFGLLGPNGAGKTT
                     LIRIINQITGPDEGEVLLENQKLKPDHVYSIGYLPEERGLYKKMAVGEQALYLAQLKG
                     MSHHDAVQKLKYWFRKFEIESWWGKKVEELSKGMQQKVQFIVTVVHEPKLLIFDEPFS
                     GFDPINVNLLKDEILELRRKGATIIFSTHNMASVEELCDNIALINKANKILDGSVKEI
                     KKLHRSNTYELHYRDFDGDLQSVLNGHFSLSEIRHEDGIHTAVIKLPPDAGSNDLLAN
                     VMPHVVITGLNEIIPSMNDIFISTVNAFNGANANDLLAD"
     gene            complement(7520..8668)
                     /gene="dnaJ"
                     /locus_tag="PKI35_08010"
     CDS             complement(7520..8668)
                     /gene="dnaJ"
                     /locus_tag="PKI35_08010"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNX44362.1"
                     /translation="MAKRDYYEVLGLSKGASADEIKKAYRQMALKYHPDKNPNNKEAE
                     DKFKEAAEAYEVLSDADKRKRYDQYGHEGLKNGFGGFQEFTNMEDIFSQFGDIFGSAF
                     GGAFSGFGGGGRQHRRVNRGTNLRVKVRLTLEEINTGVEKKLKVNKYVQCQHCHGTGS
                     QGGASFSTCSTCRGTGQVSHITSTFLGQMQTTSTCPACGGEGHIIQNKCVHCAGNGIV
                     KGEEIITVDIPAGVSEGIQLSMNGKGNAAARSGIPGDLIIQIEEARHDELQRDGNNLL
                     YDLFISFPDSALGTTVEVPTLDGKARIKIDPGTQAGKVLRLKGKGLPSLNSYGKGDLL
                     ITINIWTPKNLSRSEREILEQLQHSENFKPSPGYREKGFFDRMREYFQ"
     gene            complement(8697..9281)
                     /locus_tag="PKI35_08015"
     CDS             complement(8697..9281)
                     /locus_tag="PKI35_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNX44363.1"
                     /translation="MKVKKDKEKEVKADETAASSDNVAAEKKQVDTGAEPVNGDAEHV
                     LSAEAELKQKIDEVNDKYLRLYSEFDNYRKRTIKEKSELSRTASEEIITSLLPIIDDV
                     ERALKSIEESGSHESQKEGLHLIFNKFVGILTAKGLKEIKSAGETFNVDLHEAVAHFP
                     ASGEDMKNKVIEEVQKGYTLNDKVIRFAKVVVGI"
     gene            complement(9495..10808)
                     /gene="murA"
                     /locus_tag="PKI35_08020"
     CDS             complement(9495..10808)
                     /gene="murA"
                     /locus_tag="PKI35_08020"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNX44364.1"
                     /translation="MGSFKIEGGSRLHGEIVPQGAKNEALQIISAVLLTPEEVTVRNI
                     PDIHDVNKLIELIQLLGAKTERIDRNTCRFRADNVSPDNMMTDEFRHKAASLRGSVMI
                     LGPMLARFGKGYIPQPGGDKIGRRRLDTHMIGFLKLGASMDSDPAHNVTCLSAKKLHG
                     TYMLLDEASVTGTANLVMAAVMAEGTTTIFNAACEPYLVQLCSMLVRMGARISGIGSN
                     LLTIEGVKYLKGTEHTMLPDMIEIGSFIGLAAMTHSDVTIRNVNYQALGIIPDIFRKL
                     GVKIFTEGDNIHVPAQEHYEVETFMDGSIMTISDAPWPGFTPDLISVVLVTAIQAKGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLDRKFPLRGIRMSSPDIRAGV
                     SLLIAALSAKGTSIIDNIEQIDRGYENIDGRLRSLGARIQRIEQA"
     gene            complement(10981..11640)
                     /locus_tag="PKI35_08025"
     CDS             complement(10981..11640)
                     /locus_tag="PKI35_08025"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HNX44365.1"
                     /translation="MEYNSTREPMTISEYGRNVQNMVKYIAALDDREKRTRMARTLVN
                     ILVAMHPEMRDQTDLKHKFWDHLQIMSGFTLDVDSPYPAPPSPQDSPRPKKTGYTQEE
                     FKLRPYGKYIERMIQEAVKMDDSPEKEALVMNIANNLKKMYLNWNRDSVNDELIHSHL
                     SLLSRGLLKLKEDDKLHSTTEILKTSAINGQPTSSGSGQVKKKKFIPKKDQGRRRNFG
                     K"
     gene            11829..13121
                     /locus_tag="PKI35_08030"
     CDS             11829..13121
                     /locus_tag="PKI35_08030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNX44366.1"
                     /translation="MNKAILRLAIPNIITNITIPLLGMVDLGLMGHMGSEKYVGAIAL
                     GGMIFNFLFWGFGFLRMGTSGFTAQAYGRRDMGEASHVLARALMTAIAGGLLLIVLQW
                     PIAKGAFAVIHSSGGLESLARQYFNIRIYAAPATLCIYVFTGWFIGMQNARLPMILAI
                     SVNVLNIAFSLLFVMVFNLHSEGIAWANVASQYVGLLLGLFFVSLYLPKLRKYFNPKE
                     ALNWRAFRKFISVNKDIFIRTLCLIFTLSFFTTQSANTSDTVLAVNTLLFQFFYFFSY
                     FIDGFAYAAEALTGRYIGSGNRTMLVSVVKRLFVWGGSIALVFTVVYLAGGQYILRLL
                     TNNTETLASAKPYLFWVVLVPFITFAAFIWDGIYVGATASKAMRNSMVIITTVIFLPA
                     YYLLNPLLGNNGLWLAMMLFMGARGLLLGVMAKKAVFR"
     gene            13216..14802
                     /locus_tag="PKI35_08035"
     CDS             13216..14802
                     /locus_tag="PKI35_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44367.1"
                     /translation="MIMNKLYRLLIVVCFVPGCLFAQDWQCIRPAVTASYVDTTDLTA
                     QNAKNTMWIIHLDSVSQHQGWIYYYGYPTIRNINHYLNGAADIYCYNAFGPSRMGIAM
                     SAQGGENYFFNSAGDGIRFSTFRQPNDPWICCRITDTTRLYATVIKTDMEEVMGVHDS
                     VKYIMFQARDDAGKPVLHAMNSQIFKLSKHFGMITLYDFYTFPDVQLQNPFFEPVHIL
                     AGIDSSGTVIAQHNLTPREVYNILPGDIFHRYYGGYDPHYLLPISEEICLIQDTIWNT
                     THDSVTLKIDRYYHRFNGQPDGVHLYGRDTIFESYCVSPNEGSGLGSYPEQTVLYNDT
                     SGVLTAVNSFSQLRTMDYNARWVKSKGKSFNKIGSYCADTLFGDIVGPDHDFIIDYYI
                     AGCGGPYYEHGRTDCYHDYHSVFLKLVYFKKGTETWGTPIDTTSWGVPEKLSADTLYH
                     HLRIFPNPVTETVTAFAACDKGACLKLVVFSLAGMKVGEYPIEPGEQTLSLANYSPGM
                     YYAILYQDGKKIGFQKIAKY"
     gene            complement(14803..17130)
                     /locus_tag="PKI35_08040"
     CDS             complement(14803..17130)
                     /locus_tag="PKI35_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX44368.1"
                     /translation="MSDEILNELNEEQRRAVVCTEGPVMIIAGAGSGKTRALTYRVAH
                     LIEKGADPFHILCLTFTNKAAREMKERIMNLVGPEARNVWMGTFHSVFARILRIDGHL
                     LGYSPNYTIYDTDDSKSLLKNIVRELDLDPKIYQTGYLIGRISAAKSNLISHVDYNNT
                     PDLLEQDKTAGKERTGQIFTIYQNRLFRAGAMDFDDLLFNTNLLLRDHPDILYKYQEK
                     FRYILVDEYQDTNYSQYLIVKRLASRFQNICVVGDDAQSIYAFRGANIQNILNFKNDY
                     PEYQLFKLEQNYRSTQMIVNAANSIIANNKDQIQKRVWTANEEGQAIKLIKAATDNEE
                     GTLVANAIFETKMNNQLHNDAFAVLYRTNAQSRAIEEALRRLNIPYRIYGGMSFYDRK
                     EVKDLLAYFRLAVNPADEEALRRVINYPHRGIGDTTMQKVAIASGEANLSIWQILENA
                     ERFSLGVAGKTAAAIGSFITMIKSFGAQLHSLNAFDLAEHISKASGIVRELSSDETIE
                     GQGRMENIESLLNAVQDFTEREPGPEEETVPVRYLDEFLQEVALLTDQDNDDPNDTDR
                     VSLMTIHQAKGLEFQYVYITGMEENLFPSYMSMGSRAELEEERRLFYVALTRAEKRVT
                     LLYSESRYKWGQLSMAEPSRFLDEIDDAYIERPQRTPWKSLTSQPEQKTPPPRSFNEK
                     AGTYSVKKDPPQLGKNFKKVSGLTQSGNVAPGGNGGDDYEKIEKGMLVEHAAFGKGRV
                     INVEGAGPNKKASVQFDAVGTKQLLLRFAKLKILG"
     gene            17505..18248
                     /locus_tag="PKI35_08045"
     CDS             17505..18248
                     /locus_tag="PKI35_08045"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNX44369.1"
                     /translation="MENNFYNLDDKGHKHGSEIFRPKSLVFGLLVIAAGVLLMAKNTG
                     FLTPEVSHVIFSWEMLLVAIGVVNLFSNRSIISGLILILVGGFFLLVDFYHLPFTLWN
                     VLWPVLIILVGLSMIFGMSRLHRSHYWKRSDTASENFFEDVAVFGGGERKIFSDAFEG
                     GRLIAVFGGSKIDLSRCTLAQGEPMIEMVAVFGGTNLTVPPDWNIKVEVFNIFGGYAD
                     KRSLSQVDASKTIVLKGVAIFGGGEIKSY"
     gene            18272..19321
                     /locus_tag="PKI35_08050"
     CDS             18272..19321
                     /locus_tag="PKI35_08050"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNX44370.1"
                     /translation="MLSRFITRNIWLSYCAIWMVVAAVQVVLLYRVYGIGLKLALTDS
                     LISYVTFGLIGYGVWNMVRYSGKQKKNQPEVLAYHLAGLAFTVSFWLGACYLILNAIA
                     GNNHSYIAFLTNSLTVRALAGVFFYLLLISVFYLMMNFRELQERVEREAMLTASLREA
                     EISLLRSQIRPHFLFNSLNSVSALTMTDPAKAQEMVIKLSEFMRYSLNVADQAMSTLE
                     QELYHIGLYLDIEKVRFSDRLIFSKEIETACMAVKMPAMILQPLLENAVKHGVYTMPG
                     QAGVNLNVSCDQAFLKIVIGNDFDPDAAPRKGTGTGLGNVEKRLSALYHRNDLLKFRR
                     SERYFEVTLRIPLVS"
     gene            19339..20085
                     /locus_tag="PKI35_08055"
     CDS             19339..20085
                     /locus_tag="PKI35_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX44371.1"
                     /translation="MKLKAIIVDDEPLARDIVRKFLSGNENIEVTAECADGFDALKAI
                     RETKPDLVFLDIQMPKIDGFELLEVLEEKPGIIFTTAYDQFAIKAFEMNAVDYLLKPF
                     SKERFDAAVNKAVSRVNAKVTAPGANVDRLQQHYDASRGTIDRVVTRLGSKVTVTAVD
                     KIRYIESQDDYVMIYSESGSHLKEKTMKYFEEHLPSDTFVRIHRSYIINLSQIAAIEP
                     YSKDTHLITLKCGAKLKASSEGYKKLRAML"
     gene            20179..20919
                     /locus_tag="PKI35_08060"
     CDS             20179..20919
                     /locus_tag="PKI35_08060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HNX44372.1"
                     /translation="MHYPLLQYINFAILLAMILLVRNAWINFLGEFYGPPAWESARKS
                     KMLSPELLKAKRKYDDKVRFYNFWLQIRRIEKDGIPGDFAELGVYKGESARLIHLMAP
                     GRKLHLFDTFEGFPLADLEPETGAAAKYSVKDFADTSVNKVLRKIGDHANVEVHAGYF
                     PGSAKGLDNVVFALVNIDADLYNPVKAGLDFFYPRLSPSGVILVHDYNDQWKGLMRAV
                     DEFTETIPEAPVPVPDDDGTIMIVKSAR"
BASE COUNT         5162 a         4864 c         5350 g         5896 t
ORIGIN      
        1 ttggttttaa cgggccaatc attcaaagtc ccggcatgct tacaacccca ctgtccctgc
       61 tgtcaaaacc agtcagcccc gtgcgctgta ttgctgttgc aaaggtaatg ccaaaattaa
      121 ttgcagcctt gttttttgcc cgtaattgca aaaatgtatt gctgatcaac agctaagtca
      181 gatatacctc tgaaatattc ttttcataaa aatacttaaa aagaaaaatt tgcgttttat
      241 cgcaaactat atttgataaa aaatttcata tgaagaaaga actgattgcc gtatttataa
      301 ttcttttcat tctctcatcc gctcatgcac agcgtgtttc agtaagcggt tatcttaagg
      361 atgctgcttc aggcgagtcc ctgatcgggg caaatgtcat ggtcgacagt acgataacag
      421 gtacaaccac gaaccattat ggattttaca gcctttcgct attacccggt tctcatgtgc
      481 tgaaattcag ttatataggt tatcagccac aaaagataat tgttggcgtc ggccgtgata
      541 cccttatcag tgtaatgatg gaaccgggca ttgcaatgaa aggcattgag gtgaaaggcg
      601 acgctgtaaa tgcttcaacc cgctcatcac gcatgggcac agtcgagctc cggcttacgc
      661 agataaagaa actcccgtcg cttcttggtg aggccgatct gctgaaagct gttcagcttc
      721 ttcccggtgt gcagtcgggc aatgaaggca caagcggttt ttatgtgaga ggcggcggtc
      781 ctgaccagaa cctgatactg ctcgacggtg ttccggtgta caacgcgaac catcttttcg
      841 gttttttctc ggtatttaac aacgatgcca tcaatcacgc ggaactggtc aaaggcggat
      901 tccctgcccg ctatggcgga agattatcat cggtgcttga tatttcgatg aaagaaggcg
      961 acatgaaaaa actgaaagtt accggcacgc ttggattgat tgcgagcaaa cttaccatcg
     1021 aaggacctgt cattaaagat aaaacttctt ttattatcag tggccggcgt acctacctcg
     1081 attacatagc ccagccgctg atgcagatgg aaagtcaacg ctattccacc gaatcaggtt
     1141 attatttttc ggacctcaat gcaaaggtga accacatttt tagcaccagg gaccgtgttt
     1201 tcctgagtgt ttattccggc aatgataaat tttacgagat tcaggataca agggattatt
     1261 tatatgaagg acagatttat aaagagtccg gccagtcgaa acttggttgg ggcaatctga
     1321 ccagcgcgct gagatggact catcaataca gcggtaaact atttgggaat gccatggtta
     1381 cttacagtaa ctacaagttc tatgtaaaac aggatgctat gaattttgaa atcagtgata
     1441 ccggcgctgt tcaatcgcag tataatcttg attattacag cggtatcaga gacatttcgg
     1501 caaagtacga tctcgactat attcctcatc ccgatcatta cattaaaagc ggaatttgct
     1561 ttacccggca cattttcagg cccggagctt cggtgacaag ttcaaaaatc ggaaccggcc
     1621 aggaaacgga tgaaaaagcc ggaacggagg atatccctgt taatgaagta acctattatg
     1681 ctgaagatga ttaccgcatc actgaaaaac tgaagataaa cggcggaatg catttttcgg
     1741 gtttcatcct gccggagaag agctatttct ctgcccagcc acggatttcg gcgcgttatc
     1801 tgctcactcc ggctgtttcg ctcaaagcca gctattctta catgtcgcag tacattcact
     1861 tgcttaccaa cagtaacatc ggacttccta ccgatttatg ggttccggcg acagcttcag
     1921 ttcctccgat gaaatcttct cagtggtcat tgggattgtc ggttgatctg ccgaacgatt
     1981 tccagtttac tttcgaaacg tattacaaga ccatgaaaaa cgtcatcgaa tataaagaag
     2041 gcgcttcgtt catgtcgagt tatacgaact gggaagaaaa agttgagccg gggaaaggct
     2101 ggggttacgg aaccgaattc ttcctccaga aaaagaccgg cgctttaacg gggtggatag
     2161 gatatacctg ggcgcattcc gaccggaagt tcccgaccat caacgacggg aaagtatttc
     2221 catacaaatt cgatcgccgg catgacgttt ccctggttct gaattaccag attgatcctc
     2281 actgggatgt ttctgtcacc tgggtttacg ggaccggcac agccatcacg atgcccaccg
     2341 tcgagtatat ggtaatcaac gatatgtacg ggctttatac agtccagagt tacagcgagc
     2401 gaaacagtta cagaacggcg ccttatcaca ggcttgatct ggggtttaat catgtaaaag
     2461 agaaaaaatg gggcgagcgt acctggagct tcgggttata caatgcttac aaccgcaaaa
     2521 atcctttcta ttaccagata gtccgcgatt actccgaaaa aaaagcatta cagcgcgttg
     2581 ccctttttcc gatcattccg tcggtaagtt acacatttaa aattggtaaa taactaaagt
     2641 tatgcgactt acatatctat tgacggccat gatgcttctt gtggccggat gtaccgaata
     2701 cgaaagcatc gaagatttcc cgattgcaga gcctgcgctt gtggccaatt cactttttca
     2761 ggccgacagc gctattacgg tttatgtgtc gagaagcctc agcattctcg acaacgctga
     2821 aatcaagccg ataacggatg ccacggtagt gatttataaa gatggactgg ctgtggatac
     2881 aatacgggaa agcaacgaat cgcaagccta tgtttcggcc atatttcccg agtcagggca
     2941 tcgatacagc gtcagggttt tccgttccgg ctatgataca gtcacctcaa catcagagtc
     3001 gttgccggcg gtcctttcag tcggcaagat tactgctgtg cctgtcgatt caaccataac
     3061 agaactctgg tatcaagaca atgtacacga tgattcggca tttacttact attcaatgga
     3121 tgttgcgtta accattaatg atccggtcaa cgaacacaac atttattccg tgatggtttt
     3181 gaaggaggat tcatcgatat tttcttcgga tttaagatat cagaccagtt cagtatattt
     3241 cacaagcaat gatatgtcat tgctcgataa tgccgatctt gaaaattccg gttcgtaccg
     3301 gactgttgcc tatttcgatg accgcaattt cgacggaaaa caatatcagt ttaaattcag
     3361 gattgaagat aatgaatgga ctactttcgc ggcaaaatac agggttttgg taagatcatt
     3421 aaccgaaacg tcatacaagt attttcggtc gctcaatagt aattacaatg agacctctcc
     3481 gtttgatgaa cctgcgcggg tttatacgaa cattcataac ggatttggaa tatttgccgg
     3541 ttatgaacga aagagttaca gaatcaggta gatagatgct tatctgaagg gttgtgcaaa
     3601 agtcttaaca tcctcggcgg taatttcctg accgcaaagg atagcgagac gttcgacgat
     3661 atttctcaat tccctgatgt tgccggtata atgtattttc ttcaattcgt cgagtgcttc
     3721 aggcttaaaa acagatggcg tttttccctg gctttcggca atcagttgca taaaatgttc
     3781 ggcaagcaat ggaatatctt cggtgcgttc gttgagggcc ggcacgtgga tgaggataac
     3841 gcttagccgg tgataaaggt cttcacggaa gttttttacg gcaatttctt cctgcatgtt
     3901 tttgttggtg gcggcaatca cgcgtacatt aacagggatt tccttttcgc cgcccacgcg
     3961 cataatcttg ttttcctgca aggcacgcaa tactttggcc tgcgccgaaa ggctcatgtc
     4021 gccgatttcg tcgagaaaga tggtgccatt gtgcgcctgc tcaaaatcgc ctttacgttg
     4081 tttgatagcc gaagtgaacg atcccttctc atgcccgaaa agaacgcttt cgatgagttc
     4141 cgaaggtatg gcggcgcagt ttacttccac gaacgggccg tcggcacggt tgctcttttc
     4201 gtgaagccag cgcgctacca gttcttttcc ggttccatta tctccggtga tcaggacgcg
     4261 ggcgtcagtc ggcgccaccc gttcaatcat gtccttgatt ttcatgatgg ccggcgattc
     4321 gccgatcatg tcgtattgcc tgctgacctt gcgtttcagc gccttggtct cggtcaccag
     4381 ccgcgacttg tccatggcat tcctgatggt gatgagcaac cggttcaggt caaggggttt
     4441 ggctatgaaa tcgtatgcgc cttttttaat ggcttccaca gccgtttcga tggtgccgtg
     4501 gcccgaaatc attaccatgg gggcatcggt tatctgctgt accttttcca gtacttcgat
     4561 accgtccatc tgcggcatct tgatatcaca aagaattacg tcaaacgttt ccttcgatac
     4621 cagttcaatt cctgacggac cgtccacggc gtcatccacc tgataatttt catattccag
     4681 gatttcacgc agggtgttac ggatagcgcg ttcatcgtca atcaccagta tacgggccat
     4741 attttaaggg ttttgagtgt taagtattaa gcagggagca aggagcaagg gagcagggag
     4801 aagggagaag ggagaaggga gaagggagaa cggaacaggg ggcagggagc atagagcaag
     4861 gagcagggag cggggagaaa ggagaaggga gagcggagaa cggagaaagg agctgggagc
     4921 aaggggcaag gagcggggag aagggtgttt gtagaataag gtcaacgctg ttcaggtatc
     4981 gtcatacttc ggactccgga ttaccctttg tacctgatcc acttccagag ttccccgtag
     5041 ttcacttttt taccatacat caggatgccg gtgcggtaaa tttttgcagc cagccaggta
     5101 gttgcgataa agccggcgat cagcaaggct gccgaaagcg ccagttgtcc gtaaggcacc
     5161 ccgaaaggta tgcgtatcat catgatgacc ggcgaggtaa acggtatcat cgaaagccac
     5221 cacgagagag agccttcagg attgttgatc acatattgca tcatcacaat agccagtata
     5281 agcggtactg tgaccggcaa catgaattgc tgcgtatccg cctcattgtc aacagccgat
     5341 ccgatagcgg caaacatcgc cccgtacagc aggtatcctc cgatgaagaa aaacaggaac
     5401 gaaagaatca tcaacgggaa gttaatggag gcgatggctt cgaaaatctg gtttgcggtt
     5461 tcgttgccgg tatcggccag caggttggct gactggtctt caccgggatt ttcgggctga
     5521 atttgttggg ttgtgacaaa tttctgctgc gcctcgggca ttttgaactt ttcggggaat
     5581 gcctgctgaa aacctccgac caaaatcgct gtaaatacta tccacaggac aaattgtgtt
     5641 aaacctacca gggcgacgcc aatgattttg ccggccatca actggaacgg cttcaccgag
     5701 gaaacgatta cttccacaat ccggctggtc ttttcctcta tcacgccacg catgagctgc
     5761 gacccgaaca tgaaaataaa caggtatatc atgatgctgg ctaccattcc tacaatcata
     5821 tttacttcgg cgaagctctt tttctcagtt ccgccttctt caatctggta ggtcagaagt
     5881 ttaacgtcgg tttttatgtt tttcaggatg gtttcactca tcaggttatc ggcggcagag
     5941 tcggccctgg cttcataacc gggagtgctg atccggatct ggttgcgaat ttcggcggcg
     6001 agcttctgct tttcgatttc acggttcatg atgcccgtga tatagctttt caggttcagc
     6061 gaaggctgct tgtcggaata aagcatcgcc tgtgaaggca cgttcatttc ggttcgcgga
     6121 atgtaaagca gggcgtatcc ttccgtttgt ttcagcgttt ttttcgcggt ttcaaggtcg
     6181 gtatactgcg gtatgaagtt gtagttgtcg gagtttacga acttgctgat gaaaagcccg
     6241 gtttcgtcaa ccacatgtac aatccgcttc tcgtcctgca tggtggcgaa ataaatggga
     6301 acgatgaaca gcgccgccat caggacaggc ccgaggattg tcatgatgat aaaggaacgt
     6361 ttcctgacgc gggtgaggta ttcacgacgt atgatgagaa atattttatt catggttctg
     6421 ggcgtttgaa agtattattt cagtcggcga gcaggtcgtt ggcgtttgcg ccgttgaaag
     6481 catttacagt gctgataaag atatcgttca tcgaagggat aatttcgttg agacctgtta
     6541 taacaacgtg cggcattaca ttggccagca ggtcattgga gccagcatca ggaggcagtt
     6601 ttatcacggc agtgtgtatg ccgtcttcat gacggatttc ggagagcgaa aaatgtccgt
     6661 tcaggaccga ttgcagatcg ccgtcaaaat cgcggtagtg caattcataa gtgttgctgc
     6721 ggtgcagctt tttgatttcc ttcacgcttc cgtccagtat tttattggct ttgttgatga
     6781 gcgcaatgtt atcgcacaac tcttccaccg aagccatgtt gtgggttgag aagatgatgg
     6841 tggcgccttt gcgcctcagc tccaggattt catccttcag caggttgacg ttgatggggt
     6901 cgaaaccgct gaacggttcg tcaaaaatga gcagtttcgg ttcatgaaca actgtcacaa
     6961 tgaactgaac tttctgttgc atgcctttcg acagttcctc aacctttttg ccccaccatg
     7021 attcgatctc aaatttcctg aaccagtatt ttagtttctg aacggcatcg tgatggctca
     7081 tgcctttgag ctgagcaagg tatagcgcct gttcccccac ggccattttc ttgtacaggc
     7141 cgcgctcttc gggcaggtag ccaatggaat aaacatgatc cggtttgagt ttctggtttt
     7201 cgaggagtac ttccccttca tcaggtccgg tgatctggtt gattatgcgg ataagcgtgg
     7261 ttttgccggc gccgttgggg ccgagcagcc caaagatgca ctgttcgggc acttcgatgc
     7321 ttactttgtt cagggccgtt tgtgtcgcaa agtgtttgct gacgctgttg acggttagaa
     7381 ttgccattat tttgacaggt tatcgggaga aaatatggtc caaagataaa ttgattcgca
     7441 gaatcatcag tgtcggtcaa acgtaaaaag ccgtgtttta ccacggcctt taacatttct
     7501 taaggattat accggtcgtt tactggaaat actcccgcat acggtcgaag aaaccttttt
     7561 cacggtatcc cggtgaaggt ttgaaatttt cggagtgctg cagttgttcg agaatttcac
     7621 gttctgaacg gctcaggttc ttaggcgtcc agatattaat ggttatcagc aggtcgcctt
     7681 tgccgtatga attgagcgaa ggcagtcctt tccccttgag cctcaggact tttccggcct
     7741 gtgttccggg gtcaatttta atacgtgcct tgccgtcaag ggtagggacc tcaaccgtgg
     7801 ttcccagggc cgaatcgggg aaactgatga acaggtcata cagcaggttg tttccgtcgc
     7861 gctgaagttc atcgtgccgg gcttcttcaa tctgtatgat gaggtcgccg ggaataccgc
     7921 tgcgggcagc ggcattccct ttgccgttca tcgaaagctg gatgccttcc gaaacaccgg
     7981 cagggatgtc aacagtgatg atttcctcgc cttttacgat accgtttccg gcgcagtgta
     8041 cacatttgtt ctgtatgatg tgtccttcgc caccgcaggc agggcaggtg gacgttgttt
     8101 gcatctgtcc caggaaagtg ctggtgatat ggcttacctg gcctgtaccg cggcaggtgg
     8161 aacaggtgga gaaggaggct ccgccttgcg aaccggtgcc atggcagtgc tggcattgaa
     8221 catatttatt gactttgagc tttttttcaa cgccggtgtt aatttcttcg agcgtcaggc
     8281 ggacctttac acgcaggtta gtgccgcggt ttacacggcg gtgctggcgg ccgccccctc
     8341 cgaaaccact gaaggcccct ccgaaagcgc tcccgaatat atcgccgaac tggctgaaaa
     8401 tgtcctccat attggtgaac tcctggaacc cgccgaaacc gtttttcagc ccttcatggc
     8461 catattggtc gtaacgtttc cgtttgtcgg catcgcttaa aacctcatag gcttcggctg
     8521 cttccttgaa tttatcttca gcctccttgt tattgggatt tttgtcggga tggtatttca
     8581 atgccatctg ccggtatgct tttttaatct catcggcgct ggcacctttg gataggccta
     8641 atacttcgta ataatctcgt tttgccactt actgtaattt ttatcggata acctgattat
     8701 attcctacta ctaccttggc aaagcgtatg actttgtcgt ttagggtgta acctttctga
     8761 acttcttcaa ttaccttgtt tttcatgtct tcgcccgatg ccgggaaatg ggcaacggct
     8821 tcgtgaaggt caacattaaa ggtctcgccg gccgatttga tctcctttaa gcctttggct
     8881 gtgagtatac caacgaattt gttgaaaata agatgtaagc cttccttttg cgattcatgg
     8941 cttccggatt cttcgatcga tttaagggcg cgttccacat catcaatgat ggggagcagc
     9001 gaggtgataa tttcttcgga ggccgtgcgg ctgagctctg atttctcttt gatcgtacgt
     9061 ttgcggtaat tgtcaaattc agaatacagc cgcaggtatt tgtcgttgac ttcgtcaatt
     9121 ttttgtttca attcagcttc agccgaaaga acatgctctg catccccgtt taccggttcg
     9181 gcgccggtat caacctgctt tttttcagcg gctacattgt cagacgatgc agccgtctca
     9241 tccgctttaa cttccttttc tttatctttt ttaactttca tgatcagaga tatcttttaa
     9301 tacactgatt ggcaacgcta taaaaacatg aaacttattt tttgcctgtt ggtgcaaaag
     9361 taaggttttg ttgcgcgtgg ttcgatcaaa taatttgcca accggggcgc cttgtcattt
     9421 tgtcaggtaa gttgatcgtg gcggaaaagt tttcctgaca aggggggcgg ttgcggggat
     9481 gcgggcccga tacgtcaggc ttgctcaatt ctttgaatcc gggcgcccag cgacctgagg
     9541 cggccatcta tattttcgta tccccggtca atttgttcta tattatcgat aatgctggtt
     9601 ccttttgccg aaagggcggc gataagcagg gaaaccccgg cgcgaatgtc cggcgacgac
     9661 atgcggatgc cgcggagcgg gaacttgcgg tcgaggccta taaccgttgc cctgtgagga
     9721 tcgcagagga tgatctgggc tcccatatcg atcagcttat ccacgaaaaa caggcggctt
     9781 tcgaacatct tctggtggat cagtacgctg cctttggcct ggatggccgt gaccagcacc
     9841 acgctgatga gatcgggggt gaagcccggc cagggggcgt cggaaatagt cataatggat
     9901 ccgtccatga aagtttccac ttcataatgc tcctgggcgg gaacatgaat gttgtcgcct
     9961 tctgtaaata ttttaacgcc tagtttccgg aatatatcgg gaatgatgcc cagggcttgg
    10021 taattcacgt tcctgatcgt gacatcggaa tgagtcatgg cggccagccc gatgaaactg
    10081 ccaatttcga tcatatcggg cagcatggta tgttcggtgc ctttcaggta tttcacgcct
    10141 tcgatggtaa gcaggtttga gccgatgccg ctgatcctgg cgcccatccg gaccagcatg
    10201 ctgcacagtt gcaccaggta aggttcgcag gcggcattga agatagtcgt tgtcccttca
    10261 gccatcacgg cagccatcac caggttggcc gtacccgtaa cggaggcttc gtcaagcagc
    10321 atataggtgc cgtgtaattt ttttgccgaa aggcaggtga cgttatgggc aggatcggaa
    10381 tccatggatg ctcccagctt cagaaaaccg atcatgtggg tgtcgagacg cctgcgacct
    10441 atcttgtcgc cgcctggctg agggatgtat cctttgccga accttgccag cataggtccc
    10501 aatatcatga cggaaccacg aaggcttgcc gctttgtgcc tgaattcatc ggtcatcata
    10561 ttatcgggcg acacgttgtc ggccctgaaa cggcatgtgt tcctgtcgat gcgctctgtc
    10621 ttcgccccga gcagttgtat cagttcgata agtttgttta catcgtgaat atccggaata
    10681 ttcctgacgg ttacttcttc aggagtgagt aaaactgcgc tgattatctg cagggcttca
    10741 tttttagcgc cctgcggaac gatttcaccg tgtaaccggc tgccgccttc tattttaaat
    10801 gaacccatga taaggatgat tttcctgacg aaaatacgga atcggaggat tggttcggaa
    10861 aggaagacgg gaaatagttt ggatatgatg cctccccggc ctgcgaaagg ctgcgggatc
    10921 agagaagaaa acgattgtgg gtaaagataa aaatcaaaga ataccgcgat gccggaagtt
    10981 ttattttccg aaattcctcc gccggccctg gtctttcttg gggatgaatt ttttcttctt
    11041 tacctgtccg ctgcctgaac tggttggttg cccgttgatg gcgcttgttt tcaggatttc
    11101 ggtggttgaa tgcagtttgt cgtcttcttt caatttgaga agtccccgcg agagtaaaga
    11161 aagatgggaa tgaataagtt catcgttcac ggaatcgcgg ttccagttga ggtacatctt
    11221 tttcaggttg ttggcgatat tcatcaccag ggcttccttt tcagggctgt cgtccatctt
    11281 cacggcttcc tgtatcattc gttcgatgta cttcccgtaa gggcgcaatt taaactcctc
    11341 ctgcgtatag ccggtctttt tcggccgggg cgaatcctgc gggctcggag gggccggata
    11401 tggcgaatca acatccagcg taaatcccga cataatttgc aggtgatccc agaatttatg
    11461 tttcaggtcg gtctggtcgc gcatttcggg gtgcatggct accaggatgt tcaccagggt
    11521 gcgtgccata cgggtgcgct tttcgcgatc gtcgagggcg gcaatatatt tgaccatgtt
    11581 ctggacattc cgtccgtact cggagatggt cattggttcg cgggtggagt tgtattccat
    11641 aaatggggaa taaaggtggt atgatttgtt aatatgctcc cggattttca gctaacggat
    11701 gaatgataaa atgctgatta aaaatattaa gctgatcgaa ggagtaaaaa tttcaggaca
    11761 aaagtagtca aatttttcat atatgagcca tgtgaaaatt ggccatattt gcaggcttta
    11821 tgtgccttat gaacaaagct attctccggt tagccattcc taacattatc acaaatataa
    11881 ccattccttt gctcggtatg gtggacctcg ggctgatggg gcacatggga agcgaaaaat
    11941 acgtcggggc cattgcgttg ggcggcatga tattcaattt tcttttctgg ggtttcggtt
    12001 tcctgcgcat gggcacgagc ggtttcacgg cgcaggcata cggaaggcgt gatatgggcg
    12061 aagcatcgca cgtgctggct cgggcgctga tgacggccat cgccggcgga ttgttgctga
    12121 ttgtcttgca gtggccaatc gccaaaggcg cctttgctgt tattcacagc agcggcgggc
    12181 tggaaagcct ggcgcgtcag tatttcaaca tccgcattta tgcggcgccg gcaacacttt
    12241 gtatttatgt ttttacaggc tggttcatcg ggatgcagaa tgcccgcctg cccatgatac
    12301 tcgccatcag tgttaatgtg ctgaatattg ctttcagcct gttgtttgtc atggttttca
    12361 acctgcacag cgaaggaatc gcctgggcca acgtggcaag ccagtatgtg ggattgctgc
    12421 tcgggctgtt ctttgtcagc ctttatctgc cgaaactcag aaaatacttc aacccgaagg
    12481 aagcattaaa ttggagggct ttcaggaaat ttatctctgt gaacaaggat atttttattc
    12541 gtacgctgtg cctcattttt accctgtcgt tcttcaccac gcaatcggcc aatacgagcg
    12601 ataccgtgct ggcggtgaat acgttgctgt tccagttctt ttactttttc agctatttta
    12661 tcgatggttt tgcctatgct gccgaggcgc tgaccggccg ctatatcggt tcggggaaca
    12721 ggacaatgct tgtttcggtc gtgaagcgac tgttcgtctg gggcggaagc atcgcgctgg
    12781 tttttactgt agtttacctc gccggagggc aatatattct ccggcttctg accaacaaca
    12841 cagaaacgct cgcttcggca aagccatatc ttttctgggt cgtactggtt ccgttcatca
    12901 ccttcgcggc ctttatctgg gatggtattt atgtaggggc aaccgcttcg aaagccatgc
    12961 gcaacagtat ggtcatcatt accacagtca tctttctgcc ggcttattac ctgttgaatc
    13021 cactattggg aaacaatggt ttatggcttg ccatgatgct ttttatgggg gcaaggggac
    13081 tgctcctggg agtaatggcg aaaaaggcgg tattcaggta agtaagataa tagatttcag
    13141 aaaatatttc cggattgatg tagtggtatc agccgctaag attgtcatat tattaaaaat
    13201 atatatctcg ccattttgat tatgaataaa ctgtataggt tactaatcgt tgtttgtttt
    13261 gtgccgggtt gcttgtttgc gcaggactgg caatgcattc gtccagcagt tacagccagc
    13321 tatgtagata caacagatct cactgctcaa aatgcaaaga atacaatgtg gataattcac
    13381 cttgattcag taagccagca tcagggttgg atttattatt acggatatcc aacaatcagg
    13441 aatatcaacc attatctgaa cggcgctgct gatatttatt gctataatgc ttttggccct
    13501 tcgaggatgg gcatagctat gtcggctcag ggaggtgaaa actacttttt caattctgcc
    13561 ggcgacggaa tcaggtttag cacgttcagg caacccaatg atccctggat atgttgcaga
    13621 attacagata caacccggct ttatgccact gttataaaga cagatatgga agaagtcatg
    13681 ggagtgcatg attcagtaaa gtatatcatg tttcaggcca gagatgacgc cggtaagcct
    13741 gttttacacg caatgaattc gcagatattc aagctgagca agcatttcgg aatgattacc
    13801 ttgtatgatt tttatacttt tcctgatgtt cagcttcaga atcccttttt tgaaccagta
    13861 catatattgg cgggtattga tagttcggga actgtcatcg cacaacataa tcttacaccc
    13921 agggaggttt acaatatttt gccaggtgat atatttcacc ggtattacgg cggatacgat
    13981 ccccattatt tgctgcccat aagcgaagaa atatgcttga tacaagatac aatatggaat
    14041 acgactcatg atagtgtgac tttaaaaatc gacaggtatt atcacagatt caatggacag
    14101 ccagatggtg tgcaccttta tggcagagat acaatatttg aatcttattg tgtttcaccg
    14161 aacgagggtt caggtttggg cagctatccc gaacaaacgg ttctctataa tgacacgagc
    14221 ggggttctca ctgcagtgaa ctcattctct caattacgaa caatggatta caatgcccgt
    14281 tgggtaaaat caaaaggcaa atcctttaat aaaatcggct cttactgcgc agatacgctg
    14341 tttggtgata ttgtaggacc agaccacgat tttattattg attactatat tgccggctgt
    14401 ggcggtccat attatgagca tggcaggact gactgttacc atgattatca ttctgtattt
    14461 ctgaaactgg tttactttaa aaaaggtact gaaacatggg gaaccccaat tgacactact
    14521 agctggggtg ttcctgaaaa attgtccgct gatactctgt accatcatct cagaatattc
    14581 cccaatccgg taactgaaac agttacagcg ttcgctgcat gtgataaggg tgcctgttta
    14641 aaactggttg ttttctcact ggcaggaatg aaggtaggag agtacccgat agagccagga
    14701 gaacaaacac tatcactggc aaattacagc ccgggaatgt actatgcgat actttaccag
    14761 gatggtaaaa agattggttt ccagaagatt gcgaagtact gatcagccaa ggattttcag
    14821 tttggcaaac ctgagcagca actgctttgt ccctacggca tcaaactgaa cggaagcttt
    14881 tttattcggg ccggcccctt caacgttaat tacgcggcct ttgccaaaag cagcatgttc
    14941 aaccagcatg cctttttcaa tcttttcgta atcatcgccg ccatttccac ctggcgcaac
    15001 attacccgat tgcgtaaggc ccgaaacctt tttgaaattt ttccctaatt gaggcgggtc
    15061 tttttttacg gaatatgttc cggctttttc gttaaacgag cggggtgggg gagttttttg
    15121 ttctggctgg ctggtaaggg atttccaggg ggtgcgctgc gggcgttcga tgtaggcatc
    15181 gtcgatctcg tcgaggaagc ggcttggctc ggccatactg agttgtcccc atttgtaccg
    15241 tgattcggaa tataacagcg tcacccgttt ttcggcccgc gtcagggcga cgtaaaacaa
    15301 ccggcgctct tcttccagtt cggcccgcga acccatcgac atatacgacg ggaaaaggtt
    15361 ttcttccatt cccgtgatat atacatactg aaattcaagt cctttggcct ggtggatggt
    15421 catgagcgaa acccggtcgg tatcgttggg atcgtcgtta tcctggtcgg taagcagggc
    15481 tacttcctga aggaactcat ccagatatct cacaggtacg gtttcttcct cagggccggg
    15541 ttcacgttcg gtgaaatcct gcacggcatt gagcagcgat tctatgtttt ccatccgtcc
    15601 ctggccttcg atggtctcat ccgacgacag ctcgcggacg ataccggaag ccttggaaat
    15661 atgttcggca aggtcgaaag cattaaggga atgcaactgc gcgccgaagg atttgatcat
    15721 agtaatgaaa ctcccgatgg cggcagccgt tttacctgct actcccaggc tgaaccgttc
    15781 tgcattttcg agtatctgcc atattgagag gttcgcttca ccggaggcaa tggcaacctt
    15841 ttgcatggtg gtatcgccaa taccgcggtg cgggtagttt attacccggc gcaaggcttc
    15901 ttcgtcggca ggatttacag ccagcctgaa ataggcaagc aagtctttca cttccttgcg
    15961 gtcgtaaaac gacattccgc catatatcct gtaaggaata ttcaaacgcc gcaatgcttc
    16021 ctcgatggcc ctcgactggg cgtttgtccg gtaaagaacg gcaaaagcgt cgttgtgcaa
    16081 ctggttgttc atcttggttt cgaagatggc attggcaacc agtgttcctt cctcgttatc
    16141 ggtagccgct ttgatgagct tgatggcctg gccttcttcg tttgccgtcc agacgcgttt
    16201 ctgaatctgg tccttgttat tggcgatgat gctgttggcg gcattcacga tcatctgcgt
    16261 ggaacggtaa ttttgctcca gcttgaaaag ctggtattca ggatagtcat ttttaaagtt
    16321 taggatgttc tgtatgttgg cgccgcggaa tgcatagatg ctctgggcgt cgtcgcctac
    16381 cacgcagatg ttctggaagc gggatgcaag ccgtttaacg ataaggtatt gcgagtagtt
    16441 ggtgtcctgg tactcatcca cgagaatata ccggaacttt tcctggtatt tgtataaaat
    16501 gtcgggatgg tcgcgcagca gcaggttggt attaaacaga aggtcgtcga aatccatggc
    16561 gccggccctg aacaggcggt tctggtaaat ggtaaaaatc tgtccggtgc gttctttacc
    16621 ggcagtttta tcctgttcaa gaaggtcggg agtattattg taatccacat gcgagatcag
    16681 gttcgatttg gcggccgaga tgcgcccgat caggtatcct gtctggtaga ttttcgggtc
    16741 caggtcgagt tccctgacaa tgtttttcag caggcttttg ctgtcgtcgg tatcgtaaat
    16801 ggtataattc ggcgaatatc cgagcaggtg gccgtcgata cggaggattc gcgcaaaaac
    16861 gctgtgaaag gttcccatcc acacattgcg ggcttcgggg cctaccaggt tcatgatgcg
    16921 ttccttcatc tcgcgggcag ccttgttggt aaaagtcagg cagaggatat ggaacgggtc
    16981 ggcgcctttt tcaatcaggt gcgcaacccg gtaggtaagc gcacgcgttt ttcctgatcc
    17041 cgctcctgca atgatcatca ccgggccttc ggtgcatact acggcacgcc gctgttcttc
    17101 atttaattcg tttaatattt cgtcgctcac tgatccatgg ttttcggtgc aaaaatagga
    17161 ttattgcctt ttaccggcat gctattttat caacatcggt ttcaaagaac gattgcttcg
    17221 gtcggtgagg gagtgttccg gagaagttcc gggattgtct ttccgggaat ccggagattc
    17281 aagaccggcg gataagcatc agatttcatt ttaaacttta aaaacggaag cggtattgtt
    17341 ctatccttcg ggaaggacct caggctgata gatagctgaa acgtaaggcc ccaaacctca
    17401 tacgtcagcc gttcaccgac ccaaatcctg cattggccga tttcggcttt attcttacta
    17461 ttccgttagg ataaatttgt cgcataatta aaaaacgaaa aacaatggaa aacaattttt
    17521 acaacctcga tgacaaaggc cacaagcatg gttcagaaat attccgtccg aaaagccttg
    17581 tattcggatt gctggttatt gcagccgggg tgctgctgat ggcaaaaaac accggattcc
    17641 ttacgcccga agtaagccac gtcatctttt cgtgggaaat gctgctcgtc gccatcggcg
    17701 tagtaaacct cttcagcaac cgttcaatta tttcaggcct tatcctgatt ctggtgggcg
    17761 gattttttct gctggtggat ttctatcatt taccattcac cctttggaat gtcctatggc
    17821 cggttctcat catcctggtc ggcctgagca tgatcttcgg aatgtcaaga ctccacagat
    17881 cacattactg gaaaagaagc gatactgcca gtgaaaattt tttcgaggac gttgccgtgt
    17941 ttggaggcgg tgaacgtaaa atcttttccg atgcatttga aggcggcaga ttgatcgctg
    18001 ttttcggagg ctccaaaatt gatcttagcc gttgcaccct ggcccagggc gagcccatga
    18061 ttgaaatggt ggctgttttt ggcggaacta accttactgt tcctcccgac tggaacatta
    18121 aagtggaagt tttcaatatc tttggagggt acgctgataa aagatcgctc agccaggtcg
    18181 acgcttctaa aacgattgtt ttaaaaggag ttgcaatttt cggcggcggc gagatcaaga
    18241 gctattaaaa ccaaccgaaa tcagaaaacc catgctgagc cgcttcataa cccggaacat
    18301 ctggctctct tattgcgcaa tctggatggt agtggctgcc gtccaggttg tgctgcttta
    18361 tcgcgtttac ggaatcgggt taaagctggc gctgaccgac agcctgatat cctatgtgac
    18421 ttttggcctg attggctatg gcgtgtggaa tatggtccgg tattcgggaa agcagaaaaa
    18481 aaaccagccg gaggtgctgg cctaccacct ggccggtttg gcttttaccg tttctttctg
    18541 gctgggggcc tgttatctga tcctgaatgc catcgccgga aataatcatt cctatatcgc
    18601 gtttctgacc aactcgctga cggtgcgggc gctggcaggc gtttttttct atctgttgct
    18661 gatttcggtg ttttacctga tgatgaattt cagggaatta caggaacggg tggagcgcga
    18721 agccatgctg accgcttcgc tccgggaagc cgagatcagc cttttgcggt cgcagataag
    18781 gccgcatttc cttttcaaca gcctgaattc agtcagcgca ctcaccatga ccgatccggc
    18841 aaaagcgcag gagatggtca tcaagttatc ggaatttatg cggtattcgc tcaacgtggc
    18901 tgaccaggcg atgagcactc tcgaacagga attgtatcat atcggcctct atcttgatat
    18961 tgagaaagtc cgtttcagcg accggcttat cttttcgaaa gaaattgaaa cggcctgtat
    19021 ggctgtgaaa atgccggcca tgatcctgca gccgttactc gagaatgccg taaagcatgg
    19081 cgtctacacg atgcccggtc aggccggcgt gaacctgaat gtaagctgcg accaggcatt
    19141 cctgaaaatc gtcataggca acgactttga ccccgacgct gctccgcgca aaggaacggg
    19201 gacagggctg ggcaatgttg aaaagaggct ttcagccttg taccaccgta acgatctgct
    19261 gaaattcagg agatcggaac gctatttcga agtcacactc aggatacccc tcgtttcctg
    19321 atattttata ataaagccat gaaactaaaa gccatcatcg tggacgacga acccctggca
    19381 cgcgatatcg tcagaaaatt cctgtcgggg aacgaaaata ttgaagtgac ggccgaatgt
    19441 gccgacggtt tcgatgccct gaaggctatc cgtgaaacaa agcccgacct ggtttttctc
    19501 gacatccaga tgccgaagat cgatggcttc gaactgctcg aagtgcttga agaaaaaccg
    19561 gggatcattt tcaccaccgc ttacgaccag tttgctatta aggccttcga gatgaatgcc
    19621 gtggattacc tgctgaagcc cttttcgaaa gaacggttcg atgccgccgt gaacaaagcc
    19681 gtgagccggg ttaatgcaaa agtgacagca cccggtgcaa atgtcgaccg gttgcagcag
    19741 cactatgatg catcgcgcgg cacgatcgac cgggtggtga cccgcctcgg atcaaaagta
    19801 acggtgacag ccgtggataa aatccggtat atagagtcgc aggacgatta cgtgatgatc
    19861 tactcggagt cgggcagcca tctgaaggaa aaaaccatga aatatttcga agagcatctg
    19921 ccgtccgata ctttcgtgcg cattcacagg agttatatta tcaacctgtc gcagatagcc
    19981 gccattgaac cctattcaaa ggacacgcac ctcatcaccc tgaaatgcgg ggcaaagctt
    20041 aaagccagca gcgaaggata taaaaagctg agggcgatgc tctgataatt tctttctata
    20101 cataataata tacataggca atttatatat aagcagaaaa atttatgtta ttttgtagaa
    20161 ctttaatatc cgtgttttat gcattatccg cttttgcagt atatcaattt cgccattctg
    20221 ctggcgatga tcctacttgt aaggaacgcg tggatcaatt tcttaggtga attctatggg
    20281 ccgccggctt gggaatcggc ccggaaaagc aagatgcttt cgccggaatt gctgaaggcc
    20341 aaacgaaaat acgatgataa agtaaggttt tacaatttct ggcttcaaat acggcggatt
    20401 gagaaggacg gaatcccggg cgactttgcc gaacttggcg tatacaaggg cgaaagcgcc
    20461 cggcttatac acctgatggc gcccgggcgc aagctgcacc ttttcgacac tttcgaaggt
    20521 tttcccctcg ccgaccttga accggagacc ggggcggcag cgaaatactc ggtcaaagac
    20581 tttgccgata catctgttaa taaagtatta aggaagattg gcgatcatgc caacgtggaa
    20641 gttcatgccg gctattttcc cgggtcggcc aaagggctgg ataatgttgt atttgccctc
    20701 gtaaacatcg atgccgacct ttataatccg gtgaaagccg gactggattt tttctatccg
    20761 cggctttcgc ccagcggagt gatcctggtt catgactata atgaccaatg gaaaggcctg
    20821 atgagagccg ttgatgaatt cacggagaca ataccggaag cgccggttcc ggttcccgac
    20881 gatgacggca ctattatgat cgtaaaatca gcccggtgag tttcggaacc tgttgaatta
    20941 taccaggact taatattgca gcgggattgg aatgatgccg ggattcgggg cgatgacgga
    21001 tgaaatcttg ggaaaggtta cgcctgtcgg ttttaccaat gcccccggag aaaaatgaga
    21061 tgtaaccggg attattcgcg gggcaactct ttaactccgg gtaaagcagc cgccctgtga
    21121 ataaaaatac aatttcattt catccgatgg tatcttatcc cgcgaaattt agtatgttcg
    21181 tgcgatttat atatccacca gccatcaaac aaaccatcta ccttatgaaa tataaaatat
    21241 tgctctttgt atttgtcggc ctgctaattg cc
//
LOCUS       DAOPYV010000054        21142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_2564_length_21142_cov_12.090625, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000054 DAOPYV010000000
VERSION     DAOPYV010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21142
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2564_length_21142_cov_12.090625"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1867
                     /locus_tag="PKI35_08065"
     CDS             125..1867
                     /locus_tag="PKI35_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX44373.1"
                     /translation="MDQHRKKNISKQKSAAVTKTPARVNDGKWSYFLSLAIIVLSAWF
                     AMSYALKCGFVNYDDNLYVYGNLKIRNLSMQSFASFFTGNTGQYPPLVMLMFSALYSL
                     SGPDPSVYHLANLIFHVVNALLVFYLASRLNMGQIAATVAAVLFGAHPLHVESVAWIT
                     ELKDVQYTFFFLAGLLSYLRYRNDAGRLRYYYLSLALFILSCLSKGMAVVFPVALLLT
                     DYYLDGSIPKRRWLEKIPFLAVALLWGILTLVTQESMGAVGSSGYFLPRNILFAAYGL
                     MFYMVKMFVPVNLAAFYPLPAGSGFSLPAVYYAALAAVTGLGVLIFYFRKYRILVFGF
                     LFYAVNLLLVLQIIPVGMAVTADRYFYLSSVGLFLLIGAGCDYIYGNLKKFRLPVLII
                     GLALMLLMVRVSYRQTTTWKDSITLWNNVLQKADSNPRYSLAYLNRGNAYGDAGNTDG
                     AMNDYNRAIELNPRNADAYNNRGLIFAVNENYPEAEKDFSMALSIHPDQANALNNRGN
                     VRRFLGDFSGAVEDFSNAIKYKPGYADAYSNRGIVYYISGDPGRACNDWQKAAELGSP
                     AAASYIQQYCSGNK"
     gene            complement(1878..3005)
                     /locus_tag="PKI35_08070"
     CDS             complement(1878..3005)
                     /locus_tag="PKI35_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44374.1"
                     /translation="MSIEIRKVESRKELRQFIHLPAAIHSGEKNFLPPIYADDWNFFD
                     SKKNVSFASCDTILVLAWHNGKPVGRVMGIIHNKYNQLHNEKNARFGYLDCYNDREIS
                     DALIGFIENWALDHGMEKVIGPYGFSDKDVQGLLVEGFEHMPILDSACNPPYIVELLK
                     QKGYTKEVDCMIYRFPLTDELPQLYYRIIQRSNHRNEYQILEFATKKQLKPYIFPVLK
                     LVNETYDELFGFVPMTDEDIQEFVDRYMSIIDPRFVKIAVKEGVIIGFIVGLPNFTRG
                     IQQSKGYLFPVGIFQILWAQKHTRQLDLMLGAVKREFQGLGLEVAMGLSIVESARKAG
                     MEFIEVHLILETNRRMQAEMERLNLPVHKRFRVFQKSLPSK"
     gene            3536..5986
                     /gene="ccsA"
                     /locus_tag="PKI35_08075"
     CDS             3536..5986
                     /gene="ccsA"
                     /locus_tag="PKI35_08075"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNX44375.1"
                     /translation="MGYPGEHLIPGILGQSLIWISFASVIVSAVFFLPAGNGNVKGLV
                     KQRAAYLFFIFHFMALLGAILTLFYIISRHYFEYAYVWQYSASGAPLKYLISALWAGQ
                     EGSFLVWAFFQGILGMVLLLTSQEWKHWVMPVYAIGQVFLLSMLLGIRFLGIQAGNSP
                     FVLLRELAQNTGSELFTDSGYLKMIADGNGLNPLLENFWMTLHPPALFLGYAASFMPF
                     TYAMASLWRKEYRLWLRPCIKWTLVAMITLGSGILLGGRWAYESLTFGGFWSWDPVEN
                     ASLVPWLILVAALHLMIISERKKRLNTAACFFTLLSWALVVYATYLTRSGILGETSVH
                     SFGDNGQSLQLLIFCLLFFLLPMALLLARRESVPNKAAGNAVSKEFWMLTGAVLMALS
                     AFQVFITTSVPALNKILGTHLAPPADRINYYSTWQLPFALVAGFLISISQYPAYRKTS
                     PQRFLKRFFLSAFVAFSLVLLFILTDHVRRPGYAALMFSMLWAVLASADAMTGHFRKI
                     NSAGAAIAHTGFALFITGAVLAFGNPQVISRNVTGVDHDNEQLHGANAILFRDSAQSM
                     GDYRVTYSNRETKDSETSYRIDFKKESGSARGRSVFSAYPTVNRDPQMGAVYNPDTKH
                     FIDKDIFTCIVYAEPPENISDSTEYKLQSVEEIKVKDTLVVSGYSIILDSIHVRMKDE
                     DIENIAITAFFRIKGGVHGELPAVTGYNLVSGELLRKDAAIDALGVKLRFEGVSDKPQ
                     TISVGIYEKEKDFIVMKAIIYPFMNLLWAGAFIMVAGLVYTVILRMQRSARLPQSEKA
                     GLTNKQDNRSKNQKDISI"
     gene            5983..6777
                     /locus_tag="PKI35_08080"
     CDS             5983..6777
                     /locus_tag="PKI35_08080"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNX44376.1"
                     /translation="MKSVTFIGSGNVAWHLARAMHTAGYEIRCVWSRTMDSAEKLASL
                     VNARPVTELADIEAGDDIYVLCLNDEALPSIAAKLRFQESLVVHTSGTLSIDILQGMS
                     GNAGVLYPLQTLTKGVSVDISAVPFFIEASSGGSMQEMEELAASLSARVYKADSRQRL
                     QLHMAAVFACNYSNFMYTMAADLLSDSGFNFEVLSPLIAETTHKAINTGSLAAQTGPA
                     RRCDLNVMRKHIGLLASKPEYAEIYRLIARMIINRYHPGILNDAEL"
     gene            6764..7285
                     /locus_tag="PKI35_08085"
     CDS             6764..7285
                     /locus_tag="PKI35_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNX44377.1"
                     /translation="MLNYKELLLNINTFIFDYDGVLTDGTVLLMNNGDALRTANVKDG
                     YALQLAIKKNYRIAIISGAYSESVKRRFDSLRVKDVFLGVEKKIEIYDEYLERHRLSP
                     EHVLYMGDDIPDYEIMLKAGVPTCPADAAEEIKAVAKYISHFEGGRGCVRDVIEQVLK
                     IQGKWMNDDAYHW"
     gene            7382..8332
                     /locus_tag="PKI35_08090"
     CDS             7382..8332
                     /locus_tag="PKI35_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HNX44378.1"
                     /translation="MMQPRLSTIFRLIRLPNLIVIALTLSAVRYLVILPGLAGLSISP
                     GISPVAFGLIVFTTLTIAAAGYILNDCYDIETDMINRPERRVIGKDISRKSGLLVAVV
                     LFTIALLMGLTVSVLIRSALPFLVFLIAVIVAWWYAIMLKRTFLLGNLAVACMTACTI
                     GMIWLFDWLAAGSEILKGSDTRFITIIVTATMVFAWQLNLMREIVKDMEDVKGDERLM
                     CRSLPIVLGMQASKNMLAWQAILTISLLLASQAWLCMNKFYFVSVWLLLSVELPLAWF
                     VFRIGRTTSGKGFHRLSSMIRWIMLGGLLSLLVLRLNAAL"
     gene            8370..8969
                     /locus_tag="PKI35_08095"
     CDS             8370..8969
                     /locus_tag="PKI35_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HNX44379.1"
                     /translation="MNMDINHFRFLLGSKSPRRQHLLRELGIEYEIVNADVDESYPVH
                     YRGADIARYLCRLKADAFDLSGYPPNTILITADTIVWLNGECIGKPRGREDAIEMLRK
                     ISGHTHTVYSGICLRSGSREHVFHAETTVTFKPLTDEEIINYVNHHRPYDKAGSYGIQ
                     DWIGYIGITGIKGCYYNVMGFPVQKFWEELQLFTAGLNS"
     gene            9041..9685
                     /locus_tag="PKI35_08100"
     CDS             9041..9685
                     /locus_tag="PKI35_08100"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNX44380.1"
                     /translation="MKPFAGRILRYFLQGLLFTVPIALTAYALWWVFSFVDELLLDYI
                     SEYLGYRIPGLGLLILAVALTFAGFLGSSIVFNPILKYVDGLISRTPVIKILYTSFKE
                     FLSAFVGKDKKFTEPVLVKFSKDADMEKIGFVTGKDLTHLGIEENKVAVYFPYSYTFT
                     GTLFIVPAENVRPLNASPTEVMKFLVSAGVTSIQGKAGGNELDTMTEAVNNSMD"
     gene            9702..10478
                     /gene="surE"
                     /locus_tag="PKI35_08105"
     CDS             9702..10478
                     /gene="surE"
                     /locus_tag="PKI35_08105"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNX44381.1"
                     /translation="MKPTILITNDDGIAAPGLRMLIGIMRGIGNVVVVAPDKPQSGMG
                     HAVTMQTPLRIHEIAREPGYEEYSCNGTPVDCVKMGQKVILKANPDLIVSGINHGSNA
                     SVNVLYSGTMAAVLEGAMEDIPSIGFSLDDYSFSADFSHTAPYITSIAENVLRNSLPA
                     GVCLNVNIPALNGDKIRGIKVVRQAKAFWDESFEERKDPHRRNYYWLQGVFVPKDNGT
                     DTDEWALRNNYVSVVPVQIDLTAHQSLEMIRNWNLNGNNS"
     gene            10501..10818
                     /locus_tag="PKI35_08110"
     CDS             10501..10818
                     /locus_tag="PKI35_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44382.1"
                     /translation="MISGFLKRDSYVIGVILALIIPLLSAVVFLALNYLLSAQMQVFR
                     GVNDAGIILLSIGMNLIAMRFYLVKYKLDKTGRGILAVTFIMGILFFIFLHGKDLHIF
                     PVK"
     gene            10828..11958
                     /gene="lpxB"
                     /locus_tag="PKI35_08115"
     CDS             10828..11958
                     /gene="lpxB"
                     /locus_tag="PKI35_08115"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNX44383.1"
                     /translation="MRYYVIAGEASGDLHASLIIRELNKLDGDARFRAWGGDRMQAEG
                     AEIVKHYRDLAFMGFLEVALNLRTILGNIRFCKKDILKYKPDVLICVDYPGFNMRIAR
                     FAHKLGIKVIYYIAPQVWAWRQSRVHKIHKVVDKAFVVLPFEKGFHARFGYHVEFTGH
                     PLTDTIEESDASQSEYFRKNNNLADKPIIALIPGSRRQEIQRVLPVMLSVVDDFPGYQ
                     FIIGGVSTIPASYYDRFVGRKSIPVVYKQTYDLLRNADAALVTSGTATLETAMFGIPQ
                     VVCYRMSFISAIFAWMVIRVKFISLVNLILDKEAVRELIQYRCNRKTVSEELGKILND
                     ATYRQGMIDDYKNLRSGMGQHGASSRAAQIIWQFLGKSTSKH"
     gene            11973..13040
                     /locus_tag="PKI35_08120"
     CDS             11973..13040
                     /locus_tag="PKI35_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX44384.1"
                     /translation="MRNLTVLLAILPAFFTVMGCSGKQSGDQKMIPADSLNVPDTTIV
                     KAPDTLVKTTSVADPSWSDALKNEKAALQKQTENKSVPKGEIKLSGAKPNSPMRPLTT
                     LKDPVRSPELMSGLSKAEKGDMKGAIADFDAAIAKYANNYMAYFFRGKAKLELKDTDA
                     ALRDIEKCISIFPDQVTANYYAGKIYCDKKEYPRALEAFDNALKAKPTDAPSLDYRGV
                     TKAMMGRNSEAIADYDAALKADPKYTTSYYNKGTALAALKRYDEAVGCFTEALKQNTS
                     TKLCYMNRGNCYVLLKNQQAAIDDYTKLIELDPGNSDAYCNRGTAYSMTGDSRMCEDW
                     KKAASMGNKRAVDLLKQNCGK"
     gene            13136..15247
                     /locus_tag="PKI35_08125"
     CDS             13136..15247
                     /locus_tag="PKI35_08125"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNX44385.1"
                     /translation="MVYRHQFPVVRVLLPFVAGIALIMIAGPSRMFPAVVVALWALML
                     FYVIFSKQLWAYPSRWLFGAVASAFMFILGCQLTVLNDESRCPDYFSQVKGVKLFLAE
                     VAEPLTEKEHSYKTVLRIKGAVTEKGEIRCSGKILVYFRKDSTAKYPSFGETILWTGD
                     ITEIESPKNPGAFNYKKYMASANVFHQAFLKPGQWYITTAPVKWSFRGQAQGIRNYFL
                     EILKKNGFTGQEYAVAAALILGQEDDLDAETMRNYAGAGVMHILSVSGLHVGIVYMVL
                     NFLLGFLNRKRTTVVIKTLILITAIWFYALITGFSPSIARSATMFTFVSVAVLFRRNS
                     HVINSLAASALLLLAFNPYYLLNIGFQLSYISLAGIIFIYDWIYHRIEPVTRVGDQIW
                     QIVAISLAAQIATIPVSLYYFHQFPSYFLAANIVAIPLSSLVIYSGMLVMATSFMPAV
                     SNFMGIISTWLLKFLNGSIAWIESLPYAVISGIPFSINEMLILYAAIILMILAVAVRR
                     KGVFIMALSGLIIFFGYRSVMNYMAGEQRKFIVYSAGKSSVMEWICGTESYVVADSLF
                     LLDASRKDMILNRSNTLLRIRKVTCISQAGNEGSLRQAGPAAVSFGNKFLVNNKRVAL
                     LNNIPADAEAPQQLRLDYLVISGNTPFRLSDVLKLYDPGKIILDGSVPYKKSLQWLAD
                     ARQAGILIHSVRQQGAFIEDI"
     gene            complement(15291..15821)
                     /locus_tag="PKI35_08130"
     CDS             complement(15291..15821)
                     /locus_tag="PKI35_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNX44386.1"
                     /translation="MKPKTFKIVILSLLACTVLFIAMLLVIKPDKTRPRVKQVQNNGA
                     ESVPHTPVFRKDGELQFRDEKDDLLAAISVEIADNEEERIQGLMYRDSLPAMSGMLFM
                     FETEEPQSFWMKNTRFSLDIIYISAEHKIVSISRNAKPYSLESIPSAGPAMYVVEVPA
                     GFASRYSLTEKCRVVF"
     gene            complement(15866..16972)
                     /locus_tag="PKI35_08135"
     CDS             complement(15866..16972)
                     /locus_tag="PKI35_08135"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNX44387.1"
                     /translation="MSSFRNYLNIIILALLIFSVQDFIYGQEKNNQPLKKQALRQPDP
                     SRRGDKGIRIVFYNVENLFDTKHDSLKADEEYVTGGKRGWTFNRMLQKERNLSRVIMS
                     IGGWEMPEIIGMSEVENRYVLSLLLNETPLERYGYKIIHRESPDPRGIDVALLYNPDK
                     FKPLYSKPYTIRFPFDTAARTRDILYVKGLALNRDTLHLFVNHWPSRFGGYMETVRKR
                     NFVAQVLRSRVDSIMTANPGSKIVIMGDFNDEPSDESIVQYLKAGSDSVHVAKGDLYN
                     MMTGAGSSWNRGTIKSREVWNTIDQFIVSGTLLKATKGLSTTPHSVYIFDTPFLLQDD
                     EAWFGQKPFRTYNGFKYIGGYSDHLPILMDLKMN"
     gene            17235..19151
                     /locus_tag="PKI35_08140"
     CDS             17235..19151
                     /locus_tag="PKI35_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX44388.1"
                     /translation="MGLKKAGRINYIRRTLQWIILILLVYMLVRPLFDKNYLADFEAY
                     CPFGGIQALMSYLYSGSLACSMTTVQIGLGLALFIAVILFAKLFCSNICPVGTITEWF
                     GSLGRKVKVHITVKGLADRILRIFKYALLFITVYFSVTSSELFCKSFDPYFALVSGFG
                     SDVVIRYAIPALLLVVAGSFFIRQFWCKYICPLGAVTNIAVYALPAAALVLLWVILNV
                     IFGGAIHWIWLLGGICLTGFLLEAVTLRFLVFPPLRIVRDAEKCTRCKICNKNCPMGI
                     SISEAEKVQHIDCHLCTDCIAKCPVQGALSINKKWPRWIPAVVTVLMIAAALVISNRF
                     EIPTINEKWGSEEQMKSASAFEMEGLESIKCFGSSRAFANQMKEVPGVLGVSAFVRHH
                     RVKVLYDKSVISEEGIKEAIFSPVSEFFLLPSIGQRSVGSFSVGINHCFDPNDQYYLT
                     ELLRQQDGILALQTHFGEPVKATVYYDDKVTDERKIIKAIQQKELVIGEGKDKVVQRL
                     NFEPVIMKGIKPDTLPVSAFLRLFYEETDVTFNFFEKYKAGQIGQWEAAFDQATDPES
                     QEWIPYLISHASNDSGIVRFSILFTDDGPVLRIGYVKSLTTPEKISKLLNAPELLVYY
                     PDGKTEKVQNPFRI"
     gene            complement(19182..19256)
                     /locus_tag="PKI35_08145"
     tRNA            complement(19182..19256)
                     /locus_tag="PKI35_08145"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19219..19221),aa:Val,seq:gac)
     gene            19389..19583
                     /gene="rpsU"
                     /locus_tag="PKI35_08150"
     CDS             19389..19583
                     /gene="rpsU"
                     /locus_tag="PKI35_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNX44389.1"
                     /translation="MIIVPVKEGESIDRALKKLKRKFEKTGVVKELRERQKFTKPSIK
                     RREEVLHAIYVQQLQQQEEK"
     gene            19710..20606
                     /locus_tag="PKI35_08155"
     CDS             19710..20606
                     /locus_tag="PKI35_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNX44390.1"
                     /translation="MLKEKFIEYIRYEKRFSTHTITAYQHDLQQFAGFMQERYSQSDI
                     SIAGHSMIRSWLVDLLSKGITPRSVNRKLSTLKSFYRFCLRQGFIHENPMAKVISPKS
                     SKGLPVFLEKEPMEKLFSRQGFSGGFEGTRDRMILILFYTTGMRLSELCELRINSLDL
                     SKQTIKVTGKRNKERIIPAGGYLCDQLEIYIKERDKIFLKEQSDKSSDHLFITKKGKP
                     VYQRLVYDLVHQYLSGVSSLEKLSPHVLRHTFATQMLNEGADLNAIKEILGHANLAAT
                     QVYTHNTIEKLKSVYNQSHPRA"
     gene            20620..20919
                     /gene="raiA"
                     /locus_tag="PKI35_08160"
     CDS             20620..20919
                     /gene="raiA"
                     /locus_tag="PKI35_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNX44391.1"
                     /translation="MQVNINAVHFKTDKKLDQFITDKVEKLTGLYDGIVSSEVTLRLE
                     KTEPVENKVAEIKLVVRGNDLFAKKQSKSFEEATDTAIEALKRQLTRHKEKMRGE"
BASE COUNT         5413 a         4710 c         5148 g         5871 t
ORIGIN      
        1 cgcaggcttc actcatcaat cccggaggtg tacaggatat gctttttatc acgcattgcc
       61 ggaaagataa caaattgtat ttttggcttc cgcttcagcg tcttattccg gctggcagtt
      121 ccttatggat caacatcgta aaaaaaacat atcaaagcaa aaaagtgcag cagtaacaaa
      181 aacgccggca cgggtcaatg acgggaaatg gtcgtatttc ctgtcgctgg ccatcatcgt
      241 gctttcggca tggtttgcca tgagctacgc cctgaaatgc gggttcgtga actacgacga
      301 taacctgtat gtatacggga atctgaagat caggaacctt tccatgcagt catttgcttc
      361 gttttttacc ggaaacacag gccaataccc gccgctggta atgctgatgt tctcggccct
      421 ctattctctt tccggtcccg acccttctgt ttaccacctg gccaacctga tatttcacgt
      481 tgtgaatgcc ttgctggtgt tttatctggc atcgcgcctg aacatggggc agattgccgc
      541 aaccgtggcc gctgttttat tcggcgccca cccgttgcat gttgaatcgg ttgcctggat
      601 taccgaactg aaagatgttc aatacacctt tttcttcctt gccggcctgc tgagttacct
      661 tcgctaccgc aacgatgccg gcaggctccg gtattattat ctttccctgg ccctttttat
      721 tctttcatgc ctgtcgaaag gcatggccgt ggttttcccg gtggcgctgc tgctgaccga
      781 ttattacctg gacggttcaa taccgaaaag gagatggctt gaaaagatcc cgtttctggc
      841 cgttgctttg ttatggggaa tcctgacact ggttacccag gaatcgatgg gcgccgtggg
      901 cagttccggt tatttcttac cgcggaatat cctgtttgcc gcttacggtt tgatgtttta
      961 tatggtgaaa atgtttgtcc cggtcaacct ggcggccttt tatcccttac cggcaggcag
     1021 cggattttcg ctgccggcag tatattacgc ggcattggcg gcagtaaccg gcctcggcgt
     1081 tctcattttt tatttccgga aataccgtat cctggttttc ggatttctct tttatgccgt
     1141 caacctgttg ctggtacttc agattattcc tgtcggtatg gccgtgaccg ccgaccggta
     1201 cttttacctt tcctccgtcg ggctgtttct gctgatcggt gcgggatgcg attatattta
     1261 cgggaacctg aaaaaattcc gtctgcctgt tctcattatc ggattagcgc tcatgctgct
     1321 gatggtcagg gtttcctaca ggcaaaccac tacctggaag gatagcatca ctttgtggaa
     1381 taatgttttg caaaaggcag attcaaatcc ccgttattcg ctggcatacc tcaacagggg
     1441 aaatgcatac ggggatgcag gcaatacgga tggcgcaatg aacgattata accgggctat
     1501 cgaactaaac ccccggaatg ccgatgctta taacaacagg gggctgattt ttgccgtgaa
     1561 tgagaattat cctgaagcgg agaaggattt ttcgatggcg ttgtccatcc atcctgatca
     1621 ggccaatgcg ttgaataaca ggggtaatgt aaggcgattc ctcggcgatt tctcgggagc
     1681 cgttgaggac ttcagcaatg ccataaagta caagcccggt tatgccgatg cctattccaa
     1741 ccgtggaatt gtgtattata tttcgggtga tcccggacgg gcctgcaatg actggcaaaa
     1801 agcggcggag ctgggatctc cggcagccgc ttcctatata cagcagtatt gttccggaaa
     1861 taaatgagaa tttcccgcta ctttgaaggc agcgatttct gaaataccct gaaacgttta
     1921 tgaaccggta aattcaggcg ttccatttcg gcctgcatac ggcggtttgt ttcaaggata
     1981 aggtgtactt cgatgaattc catccccgct ttacgggccg attcaacgat gctcaggccc
     2041 atggcgactt cgaggccgag cccttgaaat tcacgtttca ccgctcccag catcaggtct
     2101 aactgtcggg tatgcttctg ggcccagagg atctgaaaaa tcccgacagg aaacaagtat
     2161 ccttttgact gctgaatgcc cctggtgaag ttcggaaggc ctacaataaa accgataatc
     2221 accccttctt ttaccgctat ttttacgaac cgcggatcga tgatggacat gtaccggtcg
     2281 acaaattcct gaatatcttc atccgtcatc ggaacaaaac cgaagagttc atcataggtt
     2341 tcgttcacca gtttcagcac ggggaagata tagggtttca actgcttctt ggtggcaaat
     2401 tcaaggatct ggtattcgtt acggtggttc gaccgctgga tgatcctgta ataaagctgg
     2461 ggcagttcat cggtgagcgg gaaacggtag atcatgcagt ccacctcttt tgtatagccc
     2521 ttttgcttca gcagctctac gatatacgga gggttgcagg ctgaatcgag gatcggcata
     2581 tgctcaaatc cttcaaccag taagccttgc acatctttat cgctgaaccc gtaagggccg
     2641 attactttct ccatgccatg atcaagggcc cagttctcga tgaaaccaat cagggcatcg
     2701 ctgatttcac ggtcattata gcaatcaagg tatccgaagc gggcattttt ctcgttatgg
     2761 agctggttgt atttattgtg gataatcccc atgacgcgtc ccaccggctt gccgttatgc
     2821 catgcaagca caaggatggt gtcgcaggat gcaaaagaga catttttctt cgaatcaaag
     2881 aaattccaat cgtcagcata aatgggagga aggaaatttt tctcgcccga gtgaatggcg
     2941 gctggcaggt gtataaattg acgcagttct ttacgggatt caactttcct gatttctatc
     3001 gacatactgg aaggatgatt atgtgaacta agctgttgag cagtaaccgg caaatataaa
     3061 ggtatatcat ttcaaaaaaa taaatatcac tttcattttt agataaaaat cctttaaaaa
     3121 tagatatcat tttataagta tcacaaaaac aataatatag gtaagcgtga gcgccaaggt
     3181 gcggatggat gtggcacggt aaaagatatc ctgtccctta cgggcgctat taagacttta
     3241 attattgtat gacagcatgt tactttgcct gaatccttcc ggaccggaat gtatgtgtcc
     3301 tgaaaggaac cttttgccgg taaaggaaca ctcccggcag ggagccgtga ttcatgggcc
     3361 tgaaaccgta tatgcaagtt gtcccgacaa cagcggaacc tgattttcat tggggataag
     3421 ccttatcagc ccgaagaatt cctgttcagg cgcagaagaa tatacctgca tttcaacccg
     3481 atttgttgta ttgacagatt aagtttattt ttgagaaata gaagttttga taaagatggg
     3541 ttatcctggc gagcatctta tccccggaat actggggcaa agtttgatct ggatcagctt
     3601 tgcttcggtg atcgtatcgg ctgttttttt tcttcctgcc ggtaatggca atgttaaagg
     3661 attggtgaag caacgtgcgg cctatctgtt ttttatcttt cacttcatgg cattgcttgg
     3721 ggcgatatta acgctttttt atatcatcag ccggcattac tttgaatacg cgtatgtgtg
     3781 gcaatattct gcctcagggg cgcccctgaa atatctgatt tcagccttat gggcagggca
     3841 ggaggggagt tttctggtct gggctttctt ccaggggatt ctgggcatgg ttttgcttct
     3901 tacgtcacag gaatggaagc attgggttat gccggtatac gccattggac aggtttttct
     3961 gctgtccatg ctgcttggaa tccgcttttt aggcatccag gcagggaaca gcccttttgt
     4021 attgcttcgc gagttggcac aaaatacagg ttctgaactg tttactgatt ctggttacct
     4081 gaaaatgata gctgatggta atggtttgaa tcccttgctt gaaaatttct ggatgaccct
     4141 tcacccgccg gccttattcc ttggttacgc tgcgtcattc atgcctttca cctatgcaat
     4201 ggcttcgctg tggcgaaaag aatatcgttt atggctgagg ccttgtatca aatggacgct
     4261 ggtggccatg ataaccctgg gctccggcat tctgctcgga ggccgctggg cttatgaatc
     4321 gctcaccttc ggaggattct ggtcctggga tcccgttgaa aatgcatcgc tggtaccctg
     4381 gctcatactg gtcgccgcat tgcacctgat gatcatatcg gagaggaaga aacgtttgaa
     4441 tactgccgcc tgttttttta ccctcttgtc atgggccctg gtcgtttatg ccacttatct
     4501 tacacggagc ggtatattgg gcgaaacatc ggtacattcg tttggcgaca acggtcaatc
     4561 tcttcagttg ctgattttct gcttactatt ctttctcctg ccgatggctt tgcttctggc
     4621 aaggagggaa tctgttccga ataaagctgc cggtaatgct gtttcaaaag agttctggat
     4681 gttaaccggg gctgttctga tggcgttgtc ggcattccag gtctttatta ctacttctgt
     4741 tccggcgctg aacaaaatac ttggaacaca tctggcgcct cctgccgacc gcataaacta
     4801 ttacagtacc tggcaattac cgtttgccct ggttgccgga ttcctgatct caatcagtca
     4861 atatccggct taccggaaaa cgtcaccgca acggttcctg aaaagatttt ttctctctgc
     4921 ttttgtagct ttctcccttg tgctgttgtt catcctgacg gatcacgtca ggaggcccgg
     4981 ttatgcggcc ctgatgtttt caatgctgtg ggctgttctg gcttcagcgg atgcaatgac
     5041 cgggcatttc aggaaaatta atagtgccgg agctgccata gcacataccg gttttgcgct
     5101 ttttattacg ggagccgttc tggcttttgg aaatccgcag gtgatttccc gtaatgtgac
     5161 gggcgttgat catgacaatg aacagcttca tggggccaac gccattctgt tcagggattc
     5221 ggctcaatct atgggcgatt accgggtgac ttattcaaac cgtgagacga aggacagcga
     5281 aactagctac cggattgatt ttaaaaagga atcaggctcg gcccggggcc gttcagtttt
     5341 ttccgcttat ccgacggtga acagggaccc gcagatgggc gctgtttata atccggatac
     5401 aaagcatttt attgacaagg atatttttac ctgcatagtc tatgcggaac caccggaaaa
     5461 catctcagac agtaccgagt ataagctgca atccgtcgaa gagattaaag taaaggatac
     5521 gcttgtcgtc tccggctaca gcatcatact cgattcgatt catgtccgga tgaaagatga
     5581 agacatagaa aatattgcca ttacggcatt tttcaggatt aagggcgggg tgcatggtga
     5641 actgccggcg gtaacaggtt acaacctggt aagtggcgaa ttgctgcgca aagatgccgc
     5701 tattgacgct ttgggcgtca agctccggtt cgaaggcgtt tcggataaac cgcagaccat
     5761 cagtgtaggt atttatgaga aggaaaagga ttttatcgtg atgaaagcaa taatttaccc
     5821 attcatgaat ttattatggg caggcgcctt catcatggtt gccggcctgg tttatacggt
     5881 cattctcagg atgcaaagaa gcgcccggct tccgcaatcg gaaaaagctg gcctgactaa
     5941 taaacaggat aacagaagta aaaaccagaa agatatcagc atatgaaatc cgtaactttt
     6001 attggctcgg ggaatgttgc ctggcacctg gcccgggcaa tgcatactgc aggctatgaa
     6061 atcagatgcg tgtggagcag gaccatggat tcggccgaaa agctggcatc gctggttaat
     6121 gcccggcctg tgacggaact ggcagatatt gaggccggtg atgatatcta tgtgctttgc
     6181 ctcaatgatg aagccctgcc ttccattgct gcgaaactca gattccagga aagccttgtt
     6241 gttcatacat cgggcacttt aagtattgac attcttcagg gcatgtccgg gaatgccggc
     6301 gttctatacc cgttacaaac gcttaccaaa ggcgtatccg ttgatatttc agccgtgccg
     6361 ttctttatcg aggcttcttc ggggggcagt atgcaggaaa tggaagagct ggcggcttcc
     6421 ctgtcggcaa gggtttataa agccgattcc cggcaacggt tgcagcttca catggcagcg
     6481 gtgtttgcat gcaactattc caattttatg tataccatgg ctgctgatct gctttccgat
     6541 tccggtttta attttgaagt gctttccccg ttgattgccg aaacaaccca taaagcaata
     6601 aatacgggct ctttggctgc ccagaccggt ccggccaggc gttgcgattt aaatgtgatg
     6661 cgcaaacata tcggattgct tgcttcaaaa cccgaatatg ccgaaattta caggctcatt
     6721 gcacgcatga ttattaaccg ttatcacccc ggaatcctga acgatgctga attataaaga
     6781 actgcttctc aatatcaata ccttcatctt cgattacgac ggcgtactta ccgatggcac
     6841 cgtgttgctg atgaacaacg gcgatgcgtt gcgcacggcg aatgtcaaag acgggtatgc
     6901 gctccaactg gcaatcaaaa aaaattaccg gattgccatc atctcaggcg cttattccga
     6961 atcggtcaag cggcgtttcg attccttgcg ggtgaaagat gtgtttctgg gagtggaaaa
     7021 gaagatagag atttatgacg aatatctcga aaggcatcgc ctgagcccgg agcatgtcct
     7081 ttacatgggc gacgatattc cggattacga gatcatgctg aaagccggcg tgccgacttg
     7141 cccggccgat gcggcagagg aaatcaaggc tgtcgccaaa tatatttctc atttcgaggg
     7201 ggggcgtggc tgtgtgcggg atgtgattga gcaggtgctt aaaatccagg gaaaatggat
     7261 gaacgatgat gcctaccatt ggtgaccctg atcagttcca ttatttgtgc ggataccccc
     7321 ggttttcccc ggaacgctat ttcaacctgt ttaacctgat gcctaccgga tattccctta
     7381 catgatgcaa ccccgtcttt ccactatttt tcgcctgatc cgtcttccca acctgattgt
     7441 catagcattg actctcagcg ctgtgcgtta tctggtaatc ctgcccgggc ttgccggctt
     7501 aagtatatct cccgggataa gccctgtggc tttcggcctg atcgtcttca ccaccctcac
     7561 aatcgctgca gccggttata ttctgaacga ctgctatgat atagaaactg atatgatcaa
     7621 ccggccggaa cgccgggtga ttggtaaaga tatttcaagg aagtcgggtt tattggttgc
     7681 ggttgtatta tttacaatcg cccttctcat gggcctgacg gttagtgttt taatccgatc
     7741 ggcattgcca ttcctggttt tcctgatagc cgttatcgtt gcctggtggt atgccatcat
     7801 gctgaagcgg acttttttgt tgggaaacct tgctgttgcc tgtatgacgg catgcaccat
     7861 cgggatgatc tggctttttg actggctcgc agccgggtcc gaaatattaa agggctcaga
     7921 tacccggttc atcaccatca tagtgaccgc taccatggtt tttgcctggc agttgaacct
     7981 gatgcgggaa atcgtgaagg acatggaaga tgtaaaaggc gatgagcggt taatgtgccg
     8041 gtcgttgccc attgtgttgg ggatgcaggc ttcgaaaaac atgctggcat ggcaggccat
     8101 tctgacaatt tcacttttac tggcaagcca ggcctggtta tgcatgaaca aattctactt
     8161 tgtttcggtc tggctgttgc tttctgttga actgccttta gcctggtttg tttttcgaat
     8221 cggcagaacg acgtccggta aaggtttcca tcgcctcagt tcgatgatca ggtggatcat
     8281 gttgggggga ctgctttcgc tcctggtact tcgtttaaat gctgcacttt agttcaggtg
     8341 catgaaggca gataaatttt cagaacaata taaatatgga tataaaccat tttcgtttcc
     8401 tgttagggtc taaatcgccg cgcaggcagc atttgttgag ggaactgggc attgaatacg
     8461 agatagtgaa tgccgatgtg gacgaatcat accccgttca ttaccgggga gccgatattg
     8521 cccgttattt gtgcagactg aaagcagatg ccttcgattt gtcaggttat cctcccaata
     8581 caatcctcat tactgccgat actatcgtat ggctgaacgg ggaatgcatc gggaaacccc
     8641 gtggccgtga ggatgcaatt gagatgctca ggaagatatc gggacataca cacaccgttt
     8701 attcgggaat ctgtcttcga tcagggagcc gggaacatgt ttttcatgct gaaactactg
     8761 ttacgtttaa acctcttacc gacgaggaaa ttattaacta tgtaaaccat caccgcccat
     8821 acgataaagc cggttcatac ggcatacagg actggatcgg ctatatcggt attacaggca
     8881 tcaaggggtg ctactataat gtaatgggtt tccccgtgca aaaattctgg gaggaattgc
     8941 agctttttac cgcagggtta aattcataaa atcacttaac cgggcttcat tttaactctc
     9001 tgaaatcgct aattttgcac ttccttttta tcttacaaca atgaagcctt ttgccggccg
     9061 tatattacgc tatttccttc agggattgct ctttactgtg ccgatcgccc ttacggctta
     9121 cgcactctgg tgggtatttt catttgttga tgaactgctg ctcgactata tcagcgagta
     9181 cctgggatat cgtattcccg gccttggctt gctgattctt gccgttgcac tgacgtttgc
     9241 cggattcctc ggcagttcaa tcgtctttaa tccgatcctt aagtacgttg acggactgat
     9301 cagccgaacg cctgtaataa aaatactata tacctcgttt aaagaatttc tctcagcctt
     9361 tgtagggaag gataaaaaat ttaccgaacc ggtgctcgta aagttcagta aagatgccga
     9421 catggagaaa atcggctttg ttaccggtaa ggatcttacg cacctcggaa ttgaagaaaa
     9481 taaggtagcg gtttattttc cttattccta tacctttacc ggcacattgt tcattgtccc
     9541 tgccgagaat gtacgtccgc tcaacgcttc gccaaccgaa gtcatgaaat tcctggtttc
     9601 ggccggagtg accagcatcc agggcaaagc cggcggaaat gaattggata cgatgacaga
     9661 ggctgtgaac aacagtatgg attaagatat aaagacagga aatgaagcct acgatattga
     9721 taacaaatga cgacggaatt gccgcccccg gcctgaggat gctcattggc attatgcgcg
     9781 gaatcgggaa tgtggtggtt gtcgcccctg ataaaccaca atcggggatg gggcatgccg
     9841 ttaccatgca aacgcctttg cggatacatg aaatagccag agaacccggg tatgaagaat
     9901 acagttgcaa cggaacaccc gtcgattgtg ttaaaatggg tcagaaagtg atcctgaaag
     9961 caaatcccga tctgatagtt tcaggcataa accacggatc aaacgcttcc gtcaacgtgt
    10021 tgtattcagg aacgatggcc gcagtactgg aaggagccat ggaagatata ccttcgattg
    10081 gtttttcact tgatgattat tctttttccg ccgatttcag ccatacggct ccctatatta
    10141 cctccattgc tgaaaatgtg ctcaggaatt cattgccggc aggcgtttgc ctgaacgtta
    10201 atattccggc cttaaacggg gacaagatca ggggaataaa ggttgtgcga caggcaaaag
    10261 cattctggga tgaaagcttt gaggaacgaa aagaccctca tcgccgtaac tattactggc
    10321 tgcaaggcgt ttttgtgcca aaggataatg gcacagatac tgacgagtgg gcgctccgga
    10381 ataactatgt tagtgtagtg cccgttcaga ttgacctgac ggctcatcag tcccttgaaa
    10441 tgatccgtaa ctggaactta aacggcaata attcataaaa tcaaatcaaa cgttatagct
    10501 atgatctctg gtttcctgaa acgcgacagt tacgtcatcg gcgtcatcct ggcgttgata
    10561 atccctttat tgtcggctgt tgtcttcctg gcgcttaatt atttgctaag tgcgcaaatg
    10621 caggttttca ggggagtgaa cgatgcggga atcatcctgc tgtcgatagg aatgaacctg
    10681 atagcgatgc gcttttacct tgtgaaatat aaactggata agaccgggag agggattctt
    10741 gccgttacct tcatcatggg gattttattt ttcatcttcc ttcacgggaa ggacctgcac
    10801 attttccctg tcaaatagta ttatcggatg aggtactacg ttattgccgg tgaagcttcg
    10861 ggcgatttgc atgcatcgct gattatcagg gaactgaata agcttgatgg cgatgcccgt
    10921 ttcagggcct ggggcggcga cagaatgcag gctgaaggtg cggaaatcgt gaaacattat
    10981 cgtgaccttg cttttatggg attcctcgaa gttgccctga acctgaggac catcctgggc
    11041 aacatacggt tctgtaagaa agacatcctg aaatacaagc ccgatgtcct gatatgcgtg
    11101 gattaccccg gtttcaacat gcgcatcgcg cggtttgccc ataaacttgg aattaaggta
    11161 atctattata tcgcgccaca ggtttgggcc tggcggcagt cgcgggtgca taaaatccat
    11221 aaagtcgttg ataaagcttt tgtggttctg cctttcgaga agggcttcca cgcccggttc
    11281 ggataccatg tggagtttac cggacatccg cttacagata ccatcgaaga aagcgatgct
    11341 tcgcaatcgg aatatttccg taaaaacaac aacctggccg ataagccgat tattgccctt
    11401 attccgggca gccgccggca ggaaatccag cgcgtactac cggtaatgtt aagcgttgtt
    11461 gatgatttcc cgggctatca gtttattatt ggcggcgttt caaccatacc cgcatcctat
    11521 tatgaccggt ttgtcggcag gaaaagcatc ccggttgtct acaaacaaac gtacgacctg
    11581 ctgcggaatg ccgatgctgc gctggtgact tccggaaccg ctacgctcga aacggctatg
    11641 ttcggaattc cacaggtagt atgttaccgc atgagcttta tctcagcaat ttttgcctgg
    11701 atggtgataa gggtaaagtt catatcgctg gtaaacctga tcctggataa agaagccgtc
    11761 agggaactga tacaataccg ttgcaacaga aaaacggttt cggaagaact tggaaaaatt
    11821 ctgaacgatg cgacctacag gcaaggtatg atcgatgatt ataaaaatct gcgttccggg
    11881 atggggcagc atggcgcttc ctcacgtgca gcgcaaatta tctggcaatt ccttggcaag
    11941 agtacttcaa aacactaaat acctgagcga ctatgagaaa tctgactgtt ctgctggcaa
    12001 tcttacctgc attttttact gtaatgggtt gttccgggaa gcaatccggg gatcagaaaa
    12061 tgattcctgc cgatagcctt aatgttccgg atacgacaat tgttaaagcc cctgatacgc
    12121 tggttaaaac tacatccgta gcagatcctt cctggagcga tgcgctcaaa aatgaaaagg
    12181 ccgctctgca gaaacaaaca gagaacaaat ctgttccaaa aggagaaata aagctttccg
    12241 gcgcaaaacc caacagcccg atgcgtccgc tcaccacgct gaaagatccc gtgcgttctc
    12301 cggaactgat gagtggattg tcaaaagctg aaaaaggcga tatgaagggc gctatcgctg
    12361 attttgatgc ggccatcgca aaatatgcca ataattatat ggcctatttc tttcgcggca
    12421 aggcaaaact tgaactgaaa gataccgacg ccgcgctgag agatattgaa aaatgcatct
    12481 caatatttcc cgatcaggtt accgcaaatt attatgctgg taagatttat tgtgacaaga
    12541 aagaatatcc ccgggctttg gaagcattcg acaatgcttt gaaagcaaag cctaccgatg
    12601 cgccatcgct tgactaccgc ggggttacga aggccatgat gggcaggaat tcggaagcca
    12661 ttgccgatta tgatgctgcg ctgaaagccg atccgaagta taccacatcc tattataaca
    12721 aaggaactgc ccttgctgcc ctaaaaagat acgacgaagc agtcggttgc tttaccgagg
    12781 ctttgaaaca aaatacaagt actaaactct gctatatgaa tcgtggcaat tgttatgtgc
    12841 tgctgaagaa tcagcaggcc gccatcgatg attatacaaa gcttatcgaa ttagatcctg
    12901 gcaattcaga tgcatactgc aaccgcggaa ccgcttattc gatgaccgga gacagccgta
    12961 tgtgcgagga ttggaaaaaa gcagcgtcga tgggcaataa aagggctgtt gatctgctga
    13021 aacaaaattg cggaaaatag caggtttcgc agtatcaaaa tttttttttc gtttaaatcc
    13081 cggttttaca ggagtttagc atttttttct tatcttggcc tgaaatcggc aagcgttggt
    13141 ttacaggcat cagtttccgg tcgtcagggt tttactgccg tttgttgccg gcatagccct
    13201 gatcatgatt gcaggccctt cccggatgtt tccggctgtg gtggttgccc tatgggcgct
    13261 gatgctcttt tatgtgattt tctcaaaaca attatgggcc tacccgtcgc gctggctttt
    13321 cggcgctgtc gcctcagcat tcatgttcat cctgggttgc cagcttacgg tgctgaatga
    13381 tgaatcccgt tgtcccgact atttttctca ggttaaaggt gtaaaactat tcctggcaga
    13441 agttgccgaa ccgctaacag agaaagaaca ttcatataaa acggtgttaa ggattaaagg
    13501 cgctgtaacc gaaaagggag aaatccggtg ttcagggaaa atcctggttt atttccgaaa
    13561 agattcaaca gcgaaatacc catcctttgg cgagacaatt ttatggacag gagatataac
    13621 agagattgaa agcccgaaaa atccgggcgc attcaactac aaaaagtaca tggcttcggc
    13681 taatgttttt catcaggcat ttttaaagcc cgggcaatgg tacatcacca ctgctccggt
    13741 aaaatggtcg ttcagggggc aggctcaggg catccggaat tatttccttg aaatactgaa
    13801 gaagaacggt tttacaggtc aggaatatgc agtagcggca gcgctgatac tgggacagga
    13861 agacgacctt gacgcggaaa caatgcgtaa ttatgcaggg gcaggggtga tgcacatttt
    13921 aagcgtatcg ggcctgcatg taggaatcgt atatatggtg cttaattttc tgctgggatt
    13981 tctgaacaga aaaagaacaa cggtcgtcat caagacgtta atcctcataa ccgcaatctg
    14041 gttctacgcg ctgattaccg gtttctctcc ttctattgcg cgatcggcga ccatgtttac
    14101 atttgtttcg gtggcagttt tattccggcg caattcgcat gtaataaact ctttagcggc
    14161 atcggcattg ctgttgctgg cattcaaccc ctattacctc ctgaatatcg gtttccagct
    14221 ttcctacatc tcgttggcgg gaataatatt catctatgac tggatatatc atcgtattga
    14281 gccggttacc cgtgttggag atcagatatg gcaaattgtt gccatctcgc tggctgccca
    14341 gattgccaca attccggtct cgctgtacta ttttcatcaa ttccccagct atttcctggc
    14401 cgcaaatatt gttgcaattc cgttgtcaag ccttgtgatc tactcgggaa tgctggtgat
    14461 ggccacttct ttcatgccgg ccgtcagtaa ttttatgggg ataatatcaa cgtggctgct
    14521 gaagtttctg aacggatcta ttgcctggat cgaaagcctt ccttatgccg ttatttcggg
    14581 aataccgttc agtataaatg aaatgttgat cttgtatgcg gccattatac tgatgatctt
    14641 agccgttgca gttcgccgca aaggcgtttt cattatggcg ttatccgggc tgatcatctt
    14701 tttcggctac aggtctgtaa tgaattatat ggctggtgaa caacgaaaat tcatcgttta
    14761 ttcggccggg aaaagttcgg tgatggaatg gatatgcgga acagagtcgt atgtagtagc
    14821 cgattcgttg tttctgctcg atgcaagccg gaaggatatg atactgaatc gttcaaacac
    14881 tttgttgaga atcaggaaag taacctgtat ttctcaagcc ggaaatgaag gttccctgag
    14941 gcaggcaggg ccggctgccg tttcgttcgg caataaattt ctggtaaata acaaaagggt
    15001 tgccttgttg aataatattc ctgcggatgc ggaagctcct cagcaactca ggctcgatta
    15061 tctggtgatc tcaggcaata cgccgttcag gctttcggac gttttaaaac tttatgatcc
    15121 cggtaagatt atccttgacg ggagcgtgcc gtataaaaaa agccttcaat ggcttgccga
    15181 cgccaggcag gccggcatat tgattcacag cgtgcggcaa cagggcgcat ttattgagga
    15241 tatctgaaac agaaccgata atatcagcgg catgaaactt atggccggga tcagaagaca
    15301 acccggcatt tttcagtgag gctgtacctg gaggcaaagc cggcaggaac ttcaacaaca
    15361 tacatagccg ggcctgccga cgggatagat tccagtgaat aaggtttggc gtttctgctg
    15421 atgcttacaa ttttgtgttc agcactgata taaatgatgt cgagcgaaaa ccgcgtgttt
    15481 ttcatccaga acgactgcgg ttcttcggtc tcaaacatga acagcatccc gctcatggcc
    15541 gggaggctgt cgcggtacat gagcccctgt atcctttctt cttcattatc ggcaatttcc
    15601 accgaaatgg cagcaagcag gtcatccttc tcatccctga actgcagctc gccgtctttc
    15661 ctgaaaaccg gagtgtgcgg aacgctttca gcaccgttat tttggacctg tttaacgcgc
    15721 ggccgcgttt tatccggctt tattaccaga agcattgcga tgaataaaac cgtacatgcc
    15781 agcagcgaaa ggatgacaat tttgaaagtt ttcggtttca tatggttttc acctgatggt
    15841 tgatcggatt cagttccgga atgtttcagt tcattttgag gtccatgagg attggcagat
    15901 gatcgctgta acccccgata tatttaaacc cgttgtaggt cctgaaaggt ttctggccga
    15961 accatgcttc atcgtcctgc aggagaaaag gagtatcaaa gatatatacg gaatggggtg
    16021 tggttgaaag accttttgtt gcttttaaca gagtgcctga aacgataaac tggtcaatgg
    16081 tattccatac ttcgcggctt ttaatggttc ccctgttcca ggaacttccc gctccggtca
    16141 tcatattata cagatcgcct ttggcaacat gtacagaatc gctcccggct ttcaggtatt
    16201 gcactatgct ttcgtcagaa ggttcatcat taaaatcacc catgatcaca atttttgaac
    16261 cgggatttgc cgtcatgatc gaatcaaccc tgcttcgcaa aacctgggcg acaaaatttc
    16321 ttttacgtac ggtttccata taaccgccaa accgggaagg ccagtgattc acaaaaagat
    16381 gaagcgtgtc gcggtttagc gccaggcctt ttacatagag tatgtcacgg gtgcgggcag
    16441 ccgtatcaaa cgggaatcgg attgtgtatg gtttgctata caggggtttg aatttgtcgg
    16501 gattatacag cagggctaca tcaataccgc gcggatcagg tgattcgcgg tgaatgattt
    16561 tataaccata tctctccaga ggcgtttcat tgagcaacag gctcagcaca tacctgtttt
    16621 caacttcact catgccgata atttcaggca tctcccaacc tccgatgctc atgatcactc
    16681 ttgagaggtt tcgttctttt tgcagcattc ggttgaacgt ccatccccgt ttgccgccgg
    16741 ttacatactc ctcgtcggct ttcaggctgt cgtgtttggt gtcaaacagg ttttcaacat
    16801 tgtaaaacac aatccggatg cccttgtcgc cacggcggga aggatctggc tgccggagtg
    16861 cttgcttttt taaaggctga ttattcttct cctgcccgta tataaaatcc tgaacggaaa
    16921 atatcaacaa cgcaaggatg ataatattca gatagttgcg gaaactgctc atcacgtttt
    16981 gaaaatagaa atgaattgcc aaaggtagaa attatgaatg atacatttcg tcctgttatc
    17041 agggctgcaa aatcagaacc tgttcagacc gatgtgagaa aaccgccggt actttcatta
    17101 aaatcaggtg taaatgttga gaactataaa acgcttattg aatttggatt ggttctaaat
    17161 tgaaggaata acggggcttt gagcctactt tgtttaattt cgggcaaaat tttcattcat
    17221 aaacaattaa aatcatggga ttgaaaaaag ccgggcgtat aaactacata cgccggacac
    17281 tgcaatggat tatccttatc cttctggttt acatgctggt aaggcccttg ttcgataaaa
    17341 attatctggc ggatttcgaa gcgtattgtc cgtttggagg gatacaggcg cttatgagct
    17401 acctttactc aggctcgctg gcatgttcca tgacgaccgt tcagataggc cttggcctgg
    17461 cgctttttat tgctgtcatt ttgtttgcca agttattctg cagcaatatc tgtccggttg
    17521 gaaccatcac cgagtggttt ggcagtctgg gaagaaaagt aaaagttcat attacggtaa
    17581 agggacttgc tgatagaatc ctgagaattt ttaaatatgc gttgcttttt attaccgttt
    17641 atttttcggt aacgagcagt gaacttttct gcaaatcgtt tgatccgtat tttgccctgg
    17701 tatcgggttt cggcagcgat gtcgttatcc ggtatgcaat tcctgcgttg ttgctggttg
    17761 ttgccggttc ctttttcatc cgacagtttt ggtgtaaata catctgtccg ttaggcgctg
    17821 ttaccaatat agccgtgtat gctttgccgg cagcagccct ggttttgtta tgggtgatcc
    17881 tgaatgttat ttttggcggg gccattcatt ggatttggtt gttgggaggt atttgcctga
    17941 caggtttcct tcttgaagct gttaccttac gctttctggt tttcccgccc ctgcggattg
    18001 tccgggatgc tgaaaaatgc accaggtgta agatttgcaa taaaaactgc cctatgggga
    18061 taagtatttc tgaggcagaa aaagttcagc atatcgattg tcatctttgt accgattgca
    18121 tcgcaaaatg tccggtacag ggcgcacttt ccatcaataa aaaatggccc cgctggatcc
    18181 cggccgttgt gacggttctt atgattgcag cagccctcgt gatatcgaac aggtttgaaa
    18241 taccaaccat taacgaaaag tggggcagtg aggaacagat gaagtcggcg tcagcctttg
    18301 aaatggaagg cctggaaagc attaaatgct ttggcagttc gcgggcattt gccaaccaga
    18361 tgaaagaagt accgggcgtt ttgggcgtat ctgcttttgt caggcatcac cgggtaaagg
    18421 tattatatga caaatcggtt atttcggaag aaggaataaa ggaagccatc ttttccccgg
    18481 taagtgaatt cttcctgttg ccgtcaatcg ggcaaaggtc cgttggctcc ttttcggtcg
    18541 ggattaatca ttgctttgat ccgaatgacc agtattacct tactgaactg ctgaggcaac
    18601 aggacgggat ccttgctttg cagactcatt tcggggaacc ggtaaaagcc actgtttatt
    18661 atgatgacaa agtaaccgat gagcgaaaaa tcataaaggc gatacagcaa aaagaacttg
    18721 tgatcggtga aggtaaagat aaagttgtac aaagactgaa ttttgaaccg gttatcatga
    18781 aaggcataaa accggataca ctcccggttt cagcatttct caggctcttc tatgaggaaa
    18841 cggatgtcac cttcaatttc ttcgaaaaat ataaagccgg gcaaatcggg caatgggaag
    18901 cggcttttga ccaggcgacg gatccggagt cgcaggaatg gattccctac ctgatttcgc
    18961 atgcttcgaa tgatagcggt attgtacggt tcagcattct ttttacggat gacgggccgg
    19021 ttctgcggat cggctatgtg aaatcgctga ctacgcccga aaagatcagc aagttgctga
    19081 atgcacctga attgctcgtg tattatcctg atggaaaaac agaaaaggtg cagaatcctt
    19141 tcaggatata atagaaaaag cctgcatggc aggcttttaa gtgcgttgta ctggactcga
    19201 accagtgacc cccgccctgt caaggcgatg ctctgaacca actgagctaa caacgcagtt
    19261 caacggtgca aaaataataa ttcccgggaa atcaaggaaa aatatttgcg ggtcatgaaa
    19321 aattaattaa ttttgcaccc cttaaatcat taatctattc acccttaacg aaaggaagcg
    19381 tattcgctat gattattgtt cctgtaaaag aaggcgagag cattgaccgc gccctgaaga
    19441 aactgaaacg caaatttgaa aaaaccggcg tcgtaaaaga gctgcgtgaa aggcagaaat
    19501 tcaccaaacc ctccatcaag aggcgcgaag aagtcctgca cgctatttac gttcagcaac
    19561 ttcagcagca ggaagaaaaa taatcgtcgg aattttttcc gttgagcagg aagtaagtct
    19621 tgcttttctc aaaataaagt cttaatttta gcgttagcaa aagaaaagac ttgagaaagc
    19681 cgggtttact tcctgttgtt gttttttgaa tgttgaaaga gaagtttatt gaatatataa
    19741 ggtacgagaa gcggttctca acccacacca tcacggcata ccagcatgac ctgcaacaat
    19801 ttgccgggtt tatgcaggaa cgatacagcc aatccgatat ttcaattgcc ggccactcca
    19861 tgatccgttc atggctggtc gatcttctgt caaagggaat cacaccccgt tcggtaaacc
    19921 ggaaattgtc caccctgaag tccttttacc ggttttgtct gcgccaggga tttattcatg
    19981 aaaatccgat ggcgaaagtg atatcgccga agtcttcgaa aggccttccc gtatttcttg
    20041 aaaaggaacc tatggagaaa ttattctccc ggcagggatt ctcaggtggt tttgagggta
    20101 cccgtgacag gatgatcctg attctttttt acacgaccgg gatgcggcta tccgaattat
    20161 gtgaactcag gatcaattct ttagacctat caaagcaaac catcaaagtg accggcaaga
    20221 ggaataagga aagaattatt ccggcaggcg gttacctttg cgaccaattg gaaatatata
    20281 taaaggagag ggacaaaatt tttctgaaag aacaatccga caaaagcagc gaccatctgt
    20341 ttatcacaaa gaaggggaaa cccgtctacc agcggctggt ttatgatctt gttcaccaat
    20401 atctctcggg cgtgtcttcc ctcgaaaaat taagtccgca tgtgctcagg catacttttg
    20461 ctacgcaaat gctaaacgaa ggagccgatc tgaatgccat caaagaaatt cttggtcatg
    20521 caaacctggc cgctacgcag gtatatactc acaacactat agaaaaactc aaatcagtat
    20581 ataaccagtc ccatccaagg gcataaaaaa ggaggaatta tgcaagtaaa catcaatgct
    20641 gtacatttca agactgataa aaaactcgat cagttcatca ccgacaaggt ggaaaagctg
    20701 acaggactgt acgatggcat tgtgagcagc gaggtgacac tccggcttga aaagaccgaa
    20761 cccgttgaga acaaggtggc cgaaataaaa ttggtcgtgc gtggtaatga tctgtttgcc
    20821 aagaaacaaa gtaagtcgtt tgaagaagca accgatacag ccatagaagc attaaaacgt
    20881 caattaaccc gtcataagga aaaaatgcgt ggggaataat aaaaaaaaat caaatcctgt
    20941 ttgtacgttt aaattaaatg cctatttttg cagtcctttt caaaaagggc tgcatttttt
    21001 tggataaaga gttgcctttt attgataata aaggaattga cagctaagag ggattacgta
    21061 acagatactt cggcggctgt ccgccatatg ccgatgtagc tcagctggta gagcagctga
    21121 tttgtaatca gctggtcggg gg
//
LOCUS       DAOPYV010000055        21026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_2583_length_21026_cov_12.462496, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000055 DAOPYV010000000
VERSION     DAOPYV010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21026
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2583_length_21026_cov_12.462496"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            445..1059
                     /locus_tag="PKI35_08165"
     CDS             445..1059
                     /locus_tag="PKI35_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX44392.1"
                     /translation="MAENDKQTEEKIFDAATEVFLEKGLDGTRMQDIADRAGIHKSLL
                     HYYYRTKDRLFNAVFEKIAGEMFKKFAPVFDDKVALEDKIRFFLREHIAFLQKNPRLP
                     SFLLNEINRNPAIVRKFIQTLDVTRIWATLESQHKDELEKYNITSENIPQLMVSIVGM
                     SVFPFAARGLLSVILERFGGSFNDYIEKRKEYAADFVIGAIRKE"
     gene            1285..1644
                     /locus_tag="PKI35_08170"
     CDS             1285..1644
                     /locus_tag="PKI35_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX44393.1"
                     /translation="MDNKEFGKLLEERSRNFSTAIMRLSFSLPDTDEGRVVRNRIAES
                     GTNVGDNCLEILRSGSESDFRNKISWYESLAGETEYWLEIIEAMNWIESCDLQSVRLE
                     LAEMLKIFTLIRKNIKN"
     gene            1649..3028
                     /locus_tag="PKI35_08175"
     CDS             1649..3028
                     /locus_tag="PKI35_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX44394.1"
                     /translation="MKNHLFGTFDNSQYFKRLKFFRYFKYFSCFMHSKLCLPMLVLFL
                     PAQAISQKVVTLKECYEKAYAVAPVAAEKDAYREIWQLKDENLSKGWLPTLDANGSFV
                     YNSSVVDMGDILGSLPAMAGKIQPMPHEQYKITLDVNQTIYDGGAVKHARTVEKADLD
                     INQKQTEADLYKLRNQVNGYYFNILLLDRQKELLKSYLELIVRRIASVNSALASGTAL
                     KSDLDVLLSEKITLEQQITENGIRKTSFVKILSGLTGTGMDTSTEFTLPVAGENLAYA
                     INRPELQMFDLRKQQLEASLQVLQSKRMPKAFGFATLGYGNPPGSNFFKDEFAPYYIL
                     GAGVKWNIYDWNKVRNVKQIVSIQQRILDGRKEDMTENLKRLLDAKSADILSLESLLN
                     TDKELIEIRKRITAVSESKYNNGTITATEYMNELNSERQAMINYEIHKINLAMARVEY
                     INISGKEIE"
     gene            3030..3911
                     /locus_tag="PKI35_08180"
     CDS             3030..3911
                     /locus_tag="PKI35_08180"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNX44395.1"
                     /translation="MKTGLLIVPAAMVVLAGCSEKNSDADAFGTFEATEVIVSPETSG
                     KILRFNVTEGSEIDSGAEIAVIDTTLFYLQKAEIDAGMKSVSTRLATIGSQNEILSQQ
                     IENLNVNIARTGSMLKGDAATQKQYDDLTGQVAVLQKQIAANNTQKASVAAELSVQQS
                     KKATLYEQIRRCRVISPLKGTVIEKYAEAGELTAAGKPLLKVADLSLIKLKVYVSGAQ
                     LGKLKVGGKCTVRIDDGKKGYQSFPGTISYISGKAEFTPKVIQTKEERVTFVYAVTIE
                     VHNNGAMKSGMPGEAIF"
     gene            3919..4839
                     /locus_tag="PKI35_08185"
     CDS             3919..4839
                     /locus_tag="PKI35_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX44396.1"
                     /translation="MQEVIKSAGIGKKFGETQALKNITFGVDEGEIFGFIGPDGAGKT
                     TLFRIITTLLLPDEGEMTIMGLDCIRGYKELRRIIGYMPGRFSLYMDLSVEENLNFYA
                     TVFGTTVRENYDLIRDIYSHIEPFKKRLAGNLSGGMKQKLALSCALIHKPKLLVLDEP
                     TTGVDAVSRSEFWDMLKKLRKYDITIVVSTPYMDEAMRCDRVALIQEGQVLSVDEPGK
                     IREGFTRKLYTVKAPEKYKLIQALRDYPGTNTAYPFGDSVHVTFKTDGFDDTLSEYLQ
                     RMGVSGVTIEETRAGIEDRFLELMKKNANP"
     gene            4851..5588
                     /locus_tag="PKI35_08190"
     CDS             4851..5588
                     /locus_tag="PKI35_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX44397.1"
                     /translation="MTNNKVITVRNLVKKFGNFTANDNLSFEVYEGEIFGFLGANGAG
                     KTTAIRILSGLSGPTSGEVVVAGFNAKKQPEQIKKNIGYMCQKFSLYEDLTVKENIML
                     YGGIYGMRKKLIRERTDTLLAKLNFEEYGDRLISDLPLGLKQKLAFSVAVLHEPKIVF
                     LDEPTGGVDPITRRQFWEMIYETAARGITVFVTTHYMDEAEYCDRISIMNEGRIVALD
                     TPAELKRQYAADSVEEVFIKIARPANN"
     gene            5592..6698
                     /locus_tag="PKI35_08195"
     CDS             5592..6698
                     /locus_tag="PKI35_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX44398.1"
                     /translation="MKRFFGFVKKEFLHIFRDYRTMIILFGIPAAQILLFGFVVSMDL
                     KNARVAFLDLSRDEVTQKISDKLTSSGFFKRGETLLSYKDIDKVLRGNKTKAVIIFEK
                     DFGRNLIRNGRATVSIIADGSEPNMATLTTNYISAIISDYNRELAGSSAGSILVQPEV
                     RMFYNPALKGQYMFVPGVITLIMILICALMTSITITREKELGTMEALLVSPLKPVQII
                     LGKVLPYFILSFIDLIVILVLSWLVFELPVKGSLALLLAESMLYILMSLSIGILISTV
                     SKTMQQAIFISLVGMMLPTVLLSGFIFPIENMPEVYGWISALLPPRYFVVIIKNIMVK
                     GTGFLYVWKETAILVAFTLAAIGLSIRNFKIRLQ"
     gene            6718..7836
                     /locus_tag="PKI35_08200"
     CDS             6718..7836
                     /locus_tag="PKI35_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX44399.1"
                     /translation="MRTILYLIRKEFLQIFRDKFIGKAIFGIPIVQMLILVPAITFEI
                     KDIKLCIIDRDMTVESRGLVSQLAGSTFFKVRYSTFSEEEANDLLHKDKCSLILQIPS
                     GFGKEIGKGNPVKVMVSANAINASNAQLSWAYLNGVIRDYNQNIIAENAGGRAMAAIP
                     QVMVTNRYWYNEMLNYKYYMLPGILGILVLAIGFILAGLNLVKEKESGTIEQINVTPV
                     KKYQFITAKIVPFLIIGLIDLAIGLIIGILVFRIPFEGSIALLFLCSAIFLVAVLGLA
                     LFLSTFSSTQQQFMFTGFFFMIIFILMSGIFTPLESMPDWAQKIDLINPVAYIMRINR
                     MVILKGSTFQDIRGDVYALIAIAVAFTILAINRYRKTA"
     gene            complement(7855..10107)
                     /locus_tag="PKI35_08205"
     CDS             complement(7855..10107)
                     /locus_tag="PKI35_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNX44400.1"
                     /translation="MKNIDSIGHVTGRSVFTDDIPEIGGTLYAAVLASPVASGRIRML
                     DTQEASALPGVVKIFTAGDIPGENQVGGILPDETLLAEDEVHYIGQPVAFVVADSGFT
                     ARNAVKLIRVDYEERQPVTDAREAKEKGLLLFPPRTFALGNTTSAWGQCRYIFEGSAG
                     SGSQEHLYLETQGSYAWPGENNTLIVHSSTQSPALVQKTIARVLGIPMNRIEVDVRRL
                     GGGFGGKEDQATAWAAMSALAAFILQKPVKLVLSRHDDMFMTGKRHPYSSDFRIGLTE
                     DLKILAYEVTFYQNGGAAADLSPAILERTLFHATNSYFIPHVKATAYSCKTNLPPNTA
                     FRGFGAPQAMFVIESAIAMAAHELGIGANEIQQKNLMKDGDIFPYGQTPDQSHAADCW
                     DETCNSFEIDKKLNEIEAFNSSSQFIKKGMALMPVCFGISFTNTSMNQARAMVHIYQD
                     GSIGISTGAIEMGQGVNTKLKQVAAGIFSVSPSRIRLETTNTSRIANSSPTAASSGAD
                     LNGKALQIACNELLDRLKEQAAEMCNAGKGTICFRDEKVFAGETETETGWEELIISAY
                     KKRVCLSQTGHYATPVIHFDKTTEKGHPFAYYVFGTAAVTVTLDCLRGRYRTDSVQIV
                     HDFGADMNSDINRGQLEGAVVQGIGWMTMEEIRYNEKGKLLSDSLSTYKIPDIYSVPK
                     IIECKALQAEGPEMAVLGSKAIGEPPFMYGIGAYFALQNAIRAFNPRYEADYHSPMTP
                     EKALMGLYSR"
     gene            complement(10094..11527)
                     /locus_tag="PKI35_08210"
     CDS             complement(10094..11527)
                     /locus_tag="PKI35_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNX44401.1"
                     /translation="MMIEFILNDRLIQTGKPQGMPLADFIRYESGLRGTKIGCREGDC
                     GACTVMEGKNIHGNVVYKTILSCLTPLGKVHGRHIVTVEGLNGETLNPAQQAIADHSG
                     TQCGFCTPGFVVSLMTHCLSGDTSSLEKTLASLDGNICRCTGYKSLERAARQVWELNQ
                     SRNNAKPVEWMVGNRYLPAWFIDISKRLSAIEKAPVIASEDAVIIGGGTDLMVSKADE
                     LMLAGFSHPIPEVFNEIEVTSGWCRIGGSVTMSEMLHSATLKPYFPRLEEYFKRISST
                     PVRNMATVAGNLANASPIADLAIFFLALDANILLTDVRQGNKRLLPLKEFFTGYKKLN
                     LRKGEVISEVQFNLPDKESRFNFEKVSRREHLDIASVNSAILVSGEEGRITSVCLSAG
                     GVSPIPLYLGNTCEFLAGKQICAETLTEACGIIQSEIAPISDIRGSEAYKRLLLRQLF
                     LAHFIELFPDNIDIPMLISTITAGEKH"
     gene            complement(11786..16036)
                     /locus_tag="PKI35_08215"
     CDS             complement(11786..16036)
                     /locus_tag="PKI35_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNX44402.1"
                     /translation="MDTGFPDGRHTAEAVLIGFDLGSVSLNAVVMDVRFNVTENYYDW
                     VHGRPFQVFRDRLNGLLQHHPVESIKGIAITGSGGKLAAELTGGVFVNEIIAQAASTG
                     KLFPEARTVIEIGGEDSKLILLEKDPKSGQSRLADFEMNSICAAGTGSFLDQQARRIG
                     VSIENEFGEMALKSVDPPRIAGRCSVFAKSDMIHHQQIATPLHDIVAGLCFALARNFR
                     SNVARSKEIIKPVIFSGGVAANAGMVRAFREVLELADDGLVIPPYYASMGAIGAVMYA
                     SDNKLLMNHFNGLQKLGNYLAGNFSKFASLPKLTESGAEYRKEIFLSRIGREKIPVYL
                     GLDIGSLSTNVVLIDKEHHIIARRYLPTAGKPLEAIQKGLTEIYDEAGEKVEVIGAGS
                     TGSGRYLTGDFIGADAIRNEITAQATAAIDYDPTVDTIFEIGGQDSKYISIENGVVVD
                     FEMNKVCAAGTGSFLEEQAEKLDISIVEEFGNMALQSESPVKLGDRCTVFMESDLNSC
                     LQKGAKKENLVSGLAYSIVYNYLQKVVAERRIGNRIFFQGGVTNNKAVVAAFEQVLGK
                     KIIIPPHFDVTGAIGAAILAQRSMAGGQKTKFKGFGIRNADYAMSRFTCQGCANHCEI
                     RRVKIEGEKKSLYYGGRCEKYETAGRKQSKSNIPNLIAKRLEMLTGGFTENPEKKGIT
                     IGIPRALMVFYQQFPFWRTFFEELGFNIILSRESDKPLITKAIETITTETCLPVELMH
                     GHIADLIERGADYIFTPFIVNAKKTEGNPTFNCNCPWVQSYPFMVKAAFKGKIDENRL
                     LIPTLHFRFFERALVKELSEYFHEKFGLGKDKIRKAIYKADTVQVNFEKSLVEYGKEA
                     LRNIPDGCTPVILLGRPYNSTDPMLNLGLVEKLISNRVMPVPVDMMELPIVKVFGNYR
                     NMYWPNGQKLIAAARLVAADDRFHAVYLSNFRCGPDSFLRHYLTEELKGKPFLHLEVD
                     EHSADAGMVTRIEAFLDSLKGAGQNQKKEPEIFRPRPGQSAPQKSRTLYFPYMNDHAY
                     AVSAAARSCGIPSEVLPKQTEEDLALGRKYTSSKECFPMICTTGSFLKKLMEPGADPS
                     KISFFMPDHNGPCRFGQYNHFHRILFDRLGYKEAELITPSNDTSYEDVAGDQGQKFRI
                     NAWKGFVAVDFLKKLYRETKPYELSQGDCDKFYDLSLQKLVRCIENGSKGLHRLLAET
                     AAGFRNIKTDKSSRKPVVSIVGEIFMRENAFCNGNIANRLENLGVEVVVAPFSEWITY
                     STYRFTRDSRWKNDRRGIIRSKIQGAGQEIIAASLFRGLKKFTDRSKDVSLHDMLNLC
                     NTYIGKYYDGDPPIALGTAATLVNRGISGIAAILPFTCMPGTLIASVSDSFRKDHNNI
                     PFINIPYDGQDGVALETRLQAFVFQVKEYARLMKPENF"
     gene            complement(16143..17054)
                     /locus_tag="PKI35_08220"
     CDS             complement(16143..17054)
                     /locus_tag="PKI35_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX44403.1"
                     /translation="MKVSQRPKAIAMLWLTAAIWGFAFVAQRAGMVHVGPFIFNGLRF
                     LLGSLSLLPLIFWLNHREKKKVLLISPGLFRGGLAAGIVLFVAASLQQAGIVYTQAGK
                     AGFITGLYVLIVPAIGIFLGQITGRNLWIGAAVAVAGLFLLAFRGPLIIEKGDLLVLV
                     SAFFWAVHVQLINSLVKKLPALPLSAMQFAICGILSLAVSSFFETTLPGDIFKASVPL
                     LYGGLLSVGVAYTLQVVAQKYVHPAYASIILSFETVFAVIGGWLILHESMTFRNLAGC
                     LLMLTGILIVQVKDFPMGRKRNQKFQH"
     gene            17122..17841
                     /gene="pepE"
                     /locus_tag="PKI35_08225"
     CDS             17122..17841
                     /gene="pepE"
                     /locus_tag="PKI35_08225"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HNX44404.1"
                     /translation="MKLLLLSNSTNAGEEYLGWPRKLIIDFLQDYNIKTAVFIPYAGV
                     TINNNDYEARVDEVFGPMGCKITSVHKVNDPVEAVKSAECIIVGGGNTFRLVQLMYDK
                     KLMEPIRQRVMEGVPFIGWSAGSNVACPSMKTTNDMPIVQPLSLDTLNLVPFQINPHY
                     LDSNPEGHGGETREQRIIEFLEVNRKITVVGLREACALRYMDSRLHLLGNRPMRVFRY
                     HTVPVEYEPGSDIDFLLSPAI"
     gene            complement(17907..18365)
                     /locus_tag="PKI35_08230"
     CDS             complement(17907..18365)
                     /locus_tag="PKI35_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44405.1"
                     /translation="MKKTLLVTIFSIVIFTANAQDYKNSLGLAFGSPSGISFKTFLNE
                     SKALDFTVGGFSHYYVVTGLYEIHAPLSDDFKWYYGIGAHIGSWYGGNYGEGAFLGGD
                     GVIGLELKPHDIPVAFSLDVRPAVNVIGNEWDEVHHWWWWQSQLSVRYTF"
     gene            18610..19188
                     /locus_tag="PKI35_08235"
     CDS             18610..19188
                     /locus_tag="PKI35_08235"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008564302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNX44406.1"
                     /translation="MSFELPVLPYGTDALEPYISAKTIEFHYGKHHQTYVTKLNGLLA
                     GSAFENAGLGEIIRKAEGPVYNNGAQVWNHTFYWEGFSSRRGSSPQGNLLRMIDRDFG
                     SFDQFRAKFTDAAVNLFGSGWAWLVQKADGSLAIIQTGNAGNPLRDGHKPLLTCDVWE
                     HAYYLDYQNRRPDYVAAFWNLVDWDVVAARLG"
     gene            complement(19361..20626)
                     /locus_tag="PKI35_08240"
     CDS             complement(19361..20626)
                     /locus_tag="PKI35_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44407.1"
                     /translation="MKYKILLFLFAGLLLALNAKGQDFWELLPFPDVDIGDVAVNAQG
                     DIFVGTLDTYSNGVYRSTDSGQTWEQVLNTGTFQINAVAINSLGYIYAHSNGFDNLWK
                     STDNGDSWQLLNWSTASGISRFYCLGGDSLLIGCVAGGATLLRTPDGCLTFDTLYRET
                     THVAERISDIAVAPNGDIYFGLMCFFPNQGGVYKSTDQGATWQEVGLINHQVQAVEIN
                     EQGDVFIGVYYDFLQGAGGLYALYHDSTGIDTCLYGPEVNGIAINSAGDIYAGIGNPN
                     GIIRSFDNGLIFTQIDSGLPWGAMGELVADNEDYMYVCTSVPSPYLYRSAKSTITSAS
                     KSYPTLKAFNILTNPVRDRLTGIFSGSVPGNQCRAIIQSIDGRIIENKAVEVTNNYIE
                     LNVSPLLPGVYLLTVTDNKGRYTEKFIKL"
     gene            complement(20686..>21026)
                     /locus_tag="PKI35_08245"
     CDS             complement(20686..>21026)
                     /locus_tag="PKI35_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44408.1"
                     /translation="TLFRIPVFAQLINMNPDPDGPVWASGGLRAPVSSTSSYWNYIEL
                     IPTAASYGKPLPDFIYNDTLPYFSYIKTQEGNSCVQMSEVWYIYTYEINRKYGRTAAN
                     EGNDFTNLYK"
BASE COUNT         5318 a         5032 c         5321 g         5355 t
ORIGIN      
        1 acctgctgac gttttcaaaa aacaaggatt acgacaacag cacactttat ttgttcaaca
       61 atgtatacta cagggggata aataattata accttcctga taaatacacc ctcggggtga
      121 agataaaagt tcacatatag attttgaccg gccggctata ttcaccgaag ccttttatcg
      181 ccatcgggca tgtttccttt ggcataacgg ggcaatcctg atttttctcc tgctgataat
      241 ccataggttt ttattctttt gagcgtggat ggcgtattcc ggtctgactg tcgtatgtcg
      301 acaataatca gaatatcagt gtgttggagt aatgagtaaa aaaaattaaa gaaagtgtcg
      361 gatcaggaat aaattgtcgt acatttgacc agttggttaa acgttatggt taatcttaaa
      421 agttaaacct aaaagtaaaa tgtgatggcc gaaaatgata aacaaaccga agaaaagatc
      481 tttgatgctg caacggaggt attcctcgaa aaagggctgg atggaacccg catgcaggat
      541 atagccgatc gcgccggcat ccataaatcg ttgctgcact attattaccg tacgaaagac
      601 cggcttttta atgcagtatt tgaaaagatt gcaggggaga tgttcaaaaa gtttgcccct
      661 gtatttgatg ataaggttgc tttggaagat aagatcaggt ttttcctcag ggagcatatt
      721 gctttccttc agaaaaaccc caggcttccc tcgttcctcc tgaacgaaat caaccggaac
      781 ccggctattg tcagaaagtt tatccagacg cttgacgtga ccaggatatg ggctaccctg
      841 gaatcacagc acaaggatga acttgaaaaa tacaatatca cttcagagaa tatcccgcag
      901 ttgatggttt ctatcgttgg gatgagcgta ttcccgtttg cagccagggg actgctttct
      961 gtcattctgg agcggttcgg cggaagtttt aatgattata tcgagaaacg gaaagagtat
     1021 gcggctgatt ttgtgattgg ggctatcagg aaggagtgag taaagcgcct ggagtgcctg
     1081 tagttctcca ttttacgttc tgcgttttgc gttctgcgtt ttgcgttctg tgttttgcgt
     1141 tttgcgttct gtgttttacg ttctccgttt tgcgttctgt gtccttcgtt ctgtgttttg
     1201 cgttattagc gcctggagtg actgaaatgc ctggagcgat taaagtgcct gaagttagaa
     1261 atagagttac ataattgaaa agctatggat aataaagaat tcggcaaatt gctggaagaa
     1321 aggtccagga atttttcaac agctatcatg cggctgtctt tttccttgcc ggatacggat
     1381 gaaggcaggg ttgtaaggaa ccggatcgcg gaatccggaa ccaacgtcgg ggataattgc
     1441 ctggaaatac tccgttcggg gagcgaatcg gatttcagga ataaaatctc ctggtacgaa
     1501 agcctggccg gcgaaacaga atactggctg gaaatcatcg aggcgatgaa ttggatcgaa
     1561 agctgcgatc tgcagtctgt tcgccttgag ttggccgaaa tgcttaaaat cttcacctta
     1621 atcagaaaga acataaagaa ttagtttgat gaaaaatcat ttgttcggaa ccttcgataa
     1681 ttcgcagtat ttcaagcgcc taaagttctt caggtacttc aagtacttta gttgcttcat
     1741 gcactccaaa ctctgcctcc ccatgcttgt tcttttcctt ccggctcagg ccatctccca
     1801 gaaggtagtg accctgaagg aatgttacga aaaggcatat gctgtcgcgc cggtcgcagc
     1861 cgaaaaggac gcataccggg aaatctggca gcttaaggat gaaaaccttt ccaaaggatg
     1921 gctccccacg ctggatgcca acggtagctt tgtctacaac tcatcggtgg tagacatggg
     1981 tgacattctt ggttctctgc ctgctatggc cggtaaaatt caaccgatgc ctcatgagca
     2041 atataaaatt accctggatg tcaatcaaac catttatgac ggcggcgctg taaaacatgc
     2101 caggacggtt gagaaagccg acctggacat caatcagaaa cagacggaag ccgatttgta
     2161 caaactccgc aatcaggtta acgggtatta cttcaacatc ctgctccttg accgtcagaa
     2221 agaactgctg aaaagttacc tggaactgat tgtcaggcgg attgcttcag tgaattcggc
     2281 gctggcaagc ggcacggccc tgaaatccga cctggatgtt cttttatccg aaaaaattac
     2341 actggagcaa cagataacag agaacggcat caggaagacg tcctttgtga agatattgtc
     2401 cggcctcacc ggaacaggga tggatacttc gactgagttc acgcttccgg ttgctggcga
     2461 aaacctggct tatgcgataa accgccctga attgcagatg tttgacctcc ggaaacagca
     2521 actggaggca tccctgcagg ttttgcagag caagcggatg ccaaaggcct tcggctttgc
     2581 taccctgggt tacgggaatc ctccgggaag caactttttt aaagatgagt tcgcccctta
     2641 ttatatcctc ggcgccggcg taaaatggaa catctatgac tggaacaagg taaggaatgt
     2701 gaaacagata gtatccatac agcaaagaat tcttgacggt cgtaaggaag atatgacgga
     2761 aaacctgaaa aggctgcttg atgccaaaag cgcggatata ctcagtctgg aatcacttct
     2821 gaataccgac aaagaactca ttgaaatcag gaaaaggata acggctgtat cggaatcgaa
     2881 gtataataac gggaccataa cggccaccga atatatgaat gaactcaatt ccgaaaggca
     2941 ggccatgatc aattacgaaa tacataaaat caatcttgcc atggcgagag tggaatatat
     3001 aaacataagc ggaaaggaaa ttgaataata tgaaaaccgg attattaatc gtaccggcag
     3061 ccatggtggt tctggccggc tgcagtgaaa aaaacagcga tgcggatgca ttcgggactt
     3121 ttgaggcaac cgaggtaatt gtttcccccg aaaccagcgg aaaaatactg agatttaatg
     3181 taacggaagg atcggaaatt gattcagggg cggaaattgc cgttatcgac accaccttgt
     3241 tttatcttca gaaagctgaa attgatgccg gcatgaaaag tgtatcgacg cgcctggcca
     3301 cgataggttc acaaaacgaa atcctcagcc agcagatcga aaacctgaat gtaaatattg
     3361 ccaggacggg aagcatgctg aaaggtgatg ccgcaaccca gaaacagtat gatgacctga
     3421 ccggccaggt tgccgtattg caaaagcaga tagctgccaa caacacgcag aaagcttcgg
     3481 tagccgcaga attatcggta cagcaatcga aaaaagccac cctttatgag cagatcaggc
     3541 gttgccgggt gataagcccc ttgaaaggga ctgtgattga aaaatacgct gaagccgggg
     3601 aactgacggc tgccggcaag ccgctgctga aggtcgctga tttatcgctg ataaagctga
     3661 aggtttacgt gagcggcgct cagcttggga agctgaaggt tggcgggaaa tgtacggtga
     3721 ggattgatga cgggaagaaa ggctaccaat cattcccggg aacgatcagt tacatttcgg
     3781 gcaaggctga gtttaccccc aaggtcatcc agaccaaaga ggagcgtgta acctttgttt
     3841 atgccgttac aattgaagtt cataataacg gcgccatgaa atcggggatg ccgggagaag
     3901 ccatttttta agaaagtcat gcaggaagtc attaaatcgg ccggaatcgg taaaaaattc
     3961 ggggaaacgc aggccctgaa gaacatcacg ttcggggtgg atgaagggga aatctttggg
     4021 tttatcggtc ccgacggcgc cggcaaaaca acgcttttca ggataataac cacgttgctg
     4081 cttcccgacg aaggcgaaat gaccattatg gggctcgatt gcataagagg atataaagag
     4141 ctacgcagga tcatcggtta catgcccggg cggttcagcc tgtacatgga cctttcggtt
     4201 gaggagaacc tgaattttta cgccaccgtc ttcggcacta ccgttaggga gaactatgat
     4261 cttatcaggg atatttactc gcatattgag ccttttaaaa agcggcttgc cgggaatctg
     4321 tccggcggca tgaaacaaaa gcttgctttg tcatgcgccc tgatccataa accgaaattg
     4381 ctggtcctcg acgagccgac caccggcgtt gatgcggttt cgcggtcgga attctgggat
     4441 atgctgaaaa aactccggaa atacgatata acgattgttg tatcaacacc ttatatggat
     4501 gaggcgatga ggtgcgaccg ggtggcgctc atacaggaag ggcaggtcct ttcggtcgac
     4561 gagcccggga aaatccggga aggttttacc aggaaacttt acaccgtaaa agcgccggaa
     4621 aaatacaagc tcattcaggc gctgagggat tatcccggca ccaatacggc ttatcctttc
     4681 ggcgattcgg tacacgttac ttttaaaaca gacggttttg atgatacttt atccgaatat
     4741 ctgcaaagga tgggcgtttc cggtgttacc attgaggaaa ccagggctgg cattgaggac
     4801 cggtttcttg aattgatgaa gaaaaatgcc aatccataat cacgctgttt atgacaaata
     4861 ataaagtaat cacagtccgg aacctggtga aaaagtttgg caacttcact gccaacgata
     4921 acctgagctt cgaggtttat gaaggggaga ttttcggttt cctcggagca aacggcgccg
     4981 ggaagactac ggctatcagg atactctccg gtttgtccgg ccctacatcg ggagaagtgg
     5041 tcgtggccgg ctttaatgcc aaaaagcagc cggaacagat aaaaaagaac atcgggtata
     5101 tgtgccagaa gttttccctg tacgaggacc tgactgtgaa ggagaatatc atgctgtacg
     5161 gcggtattta cgggatgcgc aaaaaactga tcagggaaag gacagataca ctgctggcaa
     5221 agctcaattt tgaagaatac ggcgacaggc ttatttcgga tctcccgctg gggctgaagc
     5281 agaagctcgc cttttcggta gccgtgctgc atgagccgaa aatagtcttt ctcgatgagc
     5341 ctaccggcgg cgttgacccg attacccgtc gccagttctg ggaaatgatc tatgaaacgg
     5401 cagcccgcgg cattaccgtt tttgttacga cacattacat ggatgaagcc gagtactgcg
     5461 acaggatctc gatcatgaac gaaggaagaa tagtagcgct ggatacgccc gccgagctga
     5521 aaaggcagta tgcggcagat tcggtggagg aagtgtttat taaaattgcc cgtccggcca
     5581 acaattaaag tatgaaacgg ttcttcggat ttgtaaaaaa agagttcctg catatcttca
     5641 gggattaccg gaccatgatc atcctgttcg ggatacctgc cgcccagata ctcctgttcg
     5701 gatttgtcgt cagcatggat ttgaaaaatg cccgcgtggc ttttctggac ctttccaggg
     5761 atgaggtgac gcaaaaaatt tccgataaat tgacttcatc gggctttttc aaaagagggg
     5821 agactctgct gagttacaag gatattgata aggtgctccg cggaaacaag accaaagcgg
     5881 tgattatctt cgaaaaagac ttcggacgta accttatccg caacggcagg gcgacggtaa
     5941 gcatcatcgc cgacggctcg gaacccaata tggccaccct cacaaccaac tatatttcgg
     6001 ccatcatttc ggattataac cgggaactgg ccggttcttc agccggaagt attttggtgc
     6061 agccggaagt aaggatgttt tataatcccg ccctgaaagg acagtatatg ttcgttccag
     6121 gcgtcattac cctgattatg atcctgatct gcgccctgat gacctccatc accatcacgc
     6181 gcgaaaaaga actcggaacc atggaggcgc tgcttgtttc accgttgaaa cctgttcaga
     6241 tcattctggg caaagtgctt ccgtatttta tcctctcctt cattgattta atcgtgatcc
     6301 tggtgctttc gtggctggtg tttgagttgc cggtaaaagg gagcctggcc ctgcttttgg
     6361 ccgaatcaat gctttatatc ctgatgagcc tttcgatcgg tatcctgatc tcaacggtgt
     6421 cgaagaccat gcagcaggca atctttatct cgctggtggg tatgatgctc ccgaccgttt
     6481 tgctttccgg gttcattttc ccgatcgaaa acatgccgga agtctacgga tggatcagcg
     6541 cgttgctgcc gccaagatat ttcgtggtaa tcattaaaaa tatcatggtc aaaggaaccg
     6601 gattccttta tgtgtggaag gaaacggcaa tactcgttgc tttcacactg gcggctatcg
     6661 ggctgagcat caggaatttc aagataaggc ttcaataacg aaaacggagg aacagcgatg
     6721 aggacgatat tatacctgat acgaaaagaa tttttacaaa tcttcaggga taaatttatc
     6781 gggaaggcga ttttcggaat tcccattgtg cagatgctga tactggtgcc tgctataacc
     6841 tttgagatca aagatataaa gctgtgcatc atcgaccgcg atatgaccgt tgaatccagg
     6901 gggctggtca gccagttggc aggatccaca tttttcaagg tgcgatattc cactttttcg
     6961 gaagaagagg ctaatgattt gttgcacaag gataaatgca gtctgatact ccagatacct
     7021 tccggtttcg ggaaggagat cgggaaaggg aatcccgtaa aggtgatggt gtcggctaac
     7081 gctatcaacg cgtcaaatgc gcaactgtcg tgggcatacc tgaatggtgt gatccgcgat
     7141 tacaaccaga atattattgc cgaaaatgcg ggcggcaggg ctatggcggc catcccccag
     7201 gtgatggtta ccaaccgcta ctggtacaac gaaatgctga attacaagta ttatatgctt
     7261 cccggcatcc ttggcatcct tgtgctggca atcgggttta ttcttgccgg actgaacctt
     7321 gtcaaggaaa aggaaagcgg caccattgag cagatcaacg tcaccccggt taaaaaatac
     7381 cagttcatca ccgccaaaat agttcctttc cttatcatcg gcctcatcga cctggctatc
     7441 ggactgatca tcggcatact ggttttcagg ataccgttcg aaggcagcat tgccctgctt
     7501 tttctttgtt cggctatttt tctggtggcc gtactgggcc tggccctgtt tctttccaca
     7561 ttttcaagta cgcaacaaca attcatgttc acagggttct tctttatgat tatcttcatc
     7621 ctcatgagcg gcatttttac tccgctggag agtatgcccg actgggcgca gaagattgac
     7681 ctgatcaacc ctgtggctta tatcatgcgg atcaacagga tggtgatact gaaaggatca
     7741 acatttcagg atatccgggg tgatgtttat gccctcatcg cgattgcggt ggcgtttacc
     7801 atccttgcca tcaacaggta caggaagacg gcttaagggc tggtgatcgg atgattatcg
     7861 gctgtacagg cccatcagcg ccttctcagg cgtcatcggt gaatgataat ccgcttcgta
     7921 tcggggattg aaagccctga tggcattctg cagggcaaaa taggctccta ttccgtacat
     7981 aaaaggcggt tcgccgatgg ctttcgaacc caaaaccgcc atttcggggc cttccgcctg
     8041 taatgccttg cactcgatga tctttggaac agaataaata tcaggaattt tataggtcga
     8101 gaggctgtcg gaaagcagtt tccctttttc gttataccgg atttcttcca tcgtcatcca
     8161 gccgattccc tgcaccacgg ctccttcaag ctgcccccgg ttaatgtcgg aattcatgtc
     8221 ggcgccgaaa tcatgaacta tctgcacgct gtcggtccga taccttccgc gcaggcaatc
     8281 gagggtgacc gtgacggctg ccgtaccgaa aacataatag gcaaaaggat gccctttttc
     8341 tgttgtcttg tcaaaatgga tgacaggcgt ggcataatgg ccggtctggc tgaggcatac
     8401 tcttttttta taggctgaaa taattagttc ctcccagccg gtttccgttt cggtttcgcc
     8461 ggcgaagact ttttcgtcgc ggaagcagat cgttccctta ccggcgttac acatttctgc
     8521 ggcctgttcc tttaaccggt ccagaagttc gttgcaggca atctgcagcg cttttccgtt
     8581 caggtcggcg ccggagcttg ccgccgtggg cgaactgttg gctatgcggc tggtgttcgt
     8641 cgtttcgagc ctgatccttg acggggaaac ggaaaatatt ccggcggcaa cctgtttcag
     8701 cttggtgttg acgccctgac ccatttcgat cgcgcccgtg ctgatgccga tgctgccgtc
     8761 ctggtaaata tgcaccatgg cgcgcgcctg gttcatcgaa gtgttggtga atgaaatgcc
     8821 gaagcacacg ggcatcaggg ccatgccttt ttttatgaac tgagatgagc tgttgaaggc
     8881 ttcgatttcg ttcagttttt tatcaatttc aaaggagttg caggtttcgt cccagcaatc
     8941 cgcagcatgg gactggtcgg gcgtttgtcc gtaagggaaa atatcgccgt ccttcatcag
     9001 gtttttttgt tgtatctcat tcgccccgat gcccagttcg tgtgccgcca ttgcaatggc
     9061 cgattcgata acgaacatgg cctgtggcgc tccgaatccc ctgaaagcgg tgttgggggg
     9121 aagattggtc ttacaactgt aagccgtcgc ctttacatga ggaatgaaat aactgtttgt
     9181 ggcatgaaaa agggtgcgct ccagaatggc gggcgaaagg tcggcggcgg cgcctccgtt
     9241 ctggtaaaag gtgacctcat aggctagaat ttttaaatct tccgtcagcc cgatcctgaa
     9301 atcggaactg taaggatgac gtttgcccgt catgaacatg tcgtcatgac ggctcagcac
     9361 cagttttacc ggtttttgca ggataaacgc cgcaagcgcc gacatcgccg cccatgccgt
     9421 cgcctgatct tcttttccgc cgaaaccgcc gcccaggcgc ctgacatcca cctcgatgcg
     9481 gttcatcggt attccgagga cccgcgcaat ggtcttctgg accagggccg gactttgggt
     9541 ggaggaatgt actatgagcg tgttgttttc acccggccag gcgtaggagc cctgcgtttc
     9601 gaggtacaga tgttcctgac tgccgctgcc ggcagaacct tcaaagatat acctgcattg
     9661 tccccatgcc gaagtggtgt tgcccagagc aaaggtgcgg ggcgggaaaa gaagcaatcc
     9721 tttctctttg gcttcacggg catcggtgac aggctgccgt tcttcataat cgacccggat
     9781 gagttttacg gcgttccgtg ccgtgaatcc ggaatcggca acaacaaagg caaccggctg
     9841 gccgatataa tgtacttcgt cttcggccag cagcgtttca tccggcagga tgcccccgac
     9901 ctggttttcg cccggaatat ccccggcggt aaatattttc acgacgcccg gcaaagccga
     9961 tgcttcttgg gtatcaagca tcctgatcct gccggaggca accggtgaag ccagcacggc
    10021 tgcatacagc gtgccgccga tctccgggat atcgtccgta aaaacggaac gtccggttac
    10081 atggcctatg gaatcaatgt ttttcaccgg ctgtgatggt tgagatcagc atggggatat
    10141 caatattatc aggaaaaagt tcgatgaaat gggccaggaa gagttgcctc agcaacaatc
    10201 gtttataggc ttcgctcccc cggatatcgc tgatcggcgc tatctccgac tgtataatcc
    10261 cgcaggcttc agtgagggtt tcggcgcaga tttgttttcc ggccaggaat tcacaggtat
    10321 tgccgagata aagcgggatg ggggaaacgc cgccggccga aaggcagacg gaagtgatcc
    10381 ggccttcctc cccgctgacc aggatagcgc tgttcacgct ggcgatatca agatgttcgc
    10441 gccggctcac cttctcgaaa ttgaaacggc tttctttatc cggcaggttg aactgcactt
    10501 cgctgatcac ctcgcctttc ctgaggttca gctttttata gcctgtgaaa aactctttca
    10561 ggggcaacag ccttttattt ccctgccgga cgtcggtaag caggatgttt gcatcaaggg
    10621 ccaggaaaaa aatggccagg tcggcaatag gggaggcgtt ggcaagattt cccgctacgg
    10681 tggccatgtt ccggacggga gtggaggata tccgtttaaa atactcttca agccggggga
    10741 aatacggctt taaggttgcg gagtgcagca tttccgacat ggtcactgaa ccgccaatcc
    10801 ggcaccatcc ggaggtgact tcaatttcat tgaaaacttc agggatagga tggctgaaac
    10861 cggccagcat gagttcatcg gcttttgata ccatcaggtc ggtgccgccg ccgatgatga
    10921 cggcatcttc ggaggcaata acgggcgctt tttctatggc tgaaagccgt ttggaaatgt
    10981 caatgaacca ggcaggaagg tagcggttgc ctaccatcca ctcgactggt tttgcgttgt
    11041 ttcgggattg attcagttcc cacacctgcc tggcagccct ttcgagggac ttgtatcccg
    11101 tacaccggca aatattcccg tcgagcgatg ccagcgtttt ttcaaggctg gaagtgtcgc
    11161 ccgaaaggca gtgcgtcatg agtgaaacca caaaccccgg cgtgcaaaag ccgcattgcg
    11221 tcccggaatg atcggcaatg gcctgctgtg caggattcag ggtttccccg ttcaatcctt
    11281 ccacggtaac gatatgcctg ccgtgtactt ttccgagcgg ggtaaggcag gacaagatgg
    11341 ttttatacac gacattaccg tgaatatttt tgccttccat caccgtacac gccccgcagt
    11401 cgccttcgcg gcacccgatc tttgtccccc tgagccctga ttcgtatcgg ataaaatccg
    11461 ccagcggcat cccttgcggt ttgccggttt gtatcagccg atcgttgagg atgaattcaa
    11521 tcatcatata aaaatatgaa atttgatctt ttgttatcct cagatcaaat aaatacttcg
    11581 aaataaactt ttgatcatcg gccggctgct tttaatttca tataaaaacc actattgtcc
    11641 ggataaaacg gccatggatt gtttaaaata ttgaatatgt gtcgattaag cccctctcct
    11701 tcaggagagg ggttggggtg aggcaggtct tgattatcaa ttactttctg gtgtttcccg
    11761 gacactagtg ataaaaaacc tcatttcaga agttttccgg tttcataagc cgggcatact
    11821 cctttacctg gaagacaaaa gcctgcagcc gggtttcgag ggcgacgcca tcctggccgt
    11881 cgtaagggat gtttatgaac gggatattgt tgtgatcctt gcggaatgaa tcgctgaccg
    11941 aagcaatcag cgtacccggc atgcaggtaa acggcaggat ggccgcgatg cccgaaatgc
    12001 ccctgttgac cagggtcgcc gcagtgccca gggcgatggg cggatcgccg tcgtaatatt
    12061 tcccgatgta ggtattgcaa agattgagca tgtcgtgaag cgaaacatcc ttgctccggt
    12121 cggtgaattt tttcaggccc ctgaacaacg acgctgcaat gatctcctgc cctgcgccct
    12181 gtatcttcga acggataatg cctctccggt cgttcttcca ccggctgtcg cgggtaaacc
    12241 ggtaagtaga ataggtgatc cattctgaga aaggggcaac caccacttcc acgccgaggt
    12301 tttcgagccg gttcgcgata ttgccgttgc agaatgcgtt ctcgcgcatg aatatttcac
    12361 ctacaatcga cacgacaggt ttccgcgaac ttttatccgt ttttatgttc ctgaatccgg
    12421 cggcagtttc ggccagcaac cggtgtaatc ctttgctgcc gttttcgatg catcggacga
    12481 gcttttgcag cgaaaggtca tagaatttgt cgcaatcgcc ctggctgagc tcgtaaggtt
    12541 tggtttcacg gtagagtttc ttgaggaaat caacggctac gaatcctttc caggcgttga
    12601 tccggaactt ctgtccctgg tcgccggcaa catcttcata ggaggtgtcg ttcgaaggcg
    12661 tgatgagttc ggcctcttta tatccgagcc tgtcgaacag gatgcggtgg aaatggttat
    12721 attgcccgaa gcggcacggg ccgttgtggt cgggcatgaa gaagcttatc ttcgaagggt
    12781 cggcgccggg ttccataagc tttttcagga agcttcccgt tgtacagatc atggggaaac
    12841 actctttgga ggaagtgtac ttccggccca gggcaaggtc ctcttccgtc tgtttgggaa
    12901 gcacttccga agggataccg caactccgcg ctgccgccga tacggcataa gcatgatcgt
    12961 tcatgtaggg gaaatacagc gtgcggcttt tttgcggagc cgattgcccc ggccgcggcc
    13021 tgaaaatttc gggttccttt ttctggtttt gtccggcgcc tttcaggctg tcgaggaaag
    13081 cctcaatgcg ggtaaccatc ccggcatcag ccgaatgctc gtccacttcg aggtggagaa
    13141 aaggcttgcc tttcagctct tcggtaagat aatgccggag gaaagaatcg gggccgcacc
    13201 ggaaattgct gaggtaaacg gcatgaaatc ggtcatcggc tgcaacaagc ctggcggctg
    13261 caataagttt ttgcccgttg ggccagtaca tattcctgta attgccgaat actttgacga
    13321 tgggaagttc catcatatcg acgggaacag gcatcacccg gttgctgatg agtttctcaa
    13381 ccagaccgag gttcagcata gggtcggtgc tgttgtaagg acgccccagc aaaatcaccg
    13441 gcgtgcagcc gtcgggaata ttccggagcg cttctttgcc gtattcaaca aggcttttct
    13501 cgaagtttac ctgcaccgta tcggctttgt aaatggcttt cctgattttg tccttaccga
    13561 ggccgaactt ttcatggaaa tactccgaaa gctccttcac gagcgcccgt tcgaaaaacc
    13621 tgaagtgaag cgtcgggatg agcagcctgt tttcgtcgat tttgcccttg aaggcggctt
    13681 ttaccatgaa aggataactt tgcacccagg gacagttgca gttgaaagtg gggttgcctt
    13741 ccgttttttt tgcgttgacg atgaaagggg taaaaatgta atcggcgccc cgttcaatca
    13801 ggtcggcaat atggccatgc atcagctcca cgggaaggca ggtttcggtg gtgatggttt
    13861 caatggcttt ggtgatcagc ggtttatccg attccctcga caagatgatg ttaaaaccga
    13921 gttcttcgaa aaaggtcctc cagaaaggga attgctggta aaaaaccatc aacgccctcg
    13981 gaatgccgat ggttatgcct tttttttccg gattttcggt gaaaccgccg gtgagcattt
    14041 caagcctttt ggcaatcagg ttcgggatat tgcttttcga ttgtttccgg cctgcggttt
    14101 cgtatttttc gcatcgcccg ccatagtaca gggatttttt ctctccttcg atcttcacac
    14161 ggcgtatttc gcaatggttg gcacatccct ggcaggtgaa acggctcatg gcataatcgg
    14221 cattcctgat accgaagcct ttgaactttg ttttctgtcc cccggccata gaacgttggg
    14281 ccagtatggc cgccccgatg gcgccggtaa catcgaaatg aggcgggatg atgatctttt
    14341 tcccaagcac ctgttcaaag gcagccacca cagccttgtt gttggtgaca ccgccctgga
    14401 aaaatatccg gttgccgatg cggcgttcgg ccacgacctt ctgcaggtag ttgtaaacaa
    14461 tggaataggc cagtcccgaa accaggtttt cttttttagc gcctttctgc aggcacgagt
    14521 tcaggtccga ttccatgaaa acggtacagc ggtcgccaag tttcacgggg ctttccgact
    14581 gtaacgccat gttgccgaat tcttccacga tgctgatatc cagtttttcg gcctgttctt
    14641 cgaggaaaga accggtgccg gcagcacaaa ccttgttcat ttcgaagtct accacaacgc
    14701 cgttttcgat gctgatatac tttgaatcct gtccgccgat ttcaaagatg gtatcgacgg
    14761 tggggtcgta atcgatggcc gcggtggcct gggccgtgat ttcgttccgg atggcgtcgg
    14821 ctcctatgaa atcgccggta aggtaccttc cggaacccgt acttccggcg ccgatgactt
    14881 ctactttttc acccgcttca tcgtatattt ccgtcagccc tttttgaatg gcttcgagcg
    14941 gtttgccggc agtgggaagg taacggcggg cgatgatgtg gtgttcttta tcgatcagca
    15001 ccacgttggt gctgagtgag ccgatatcaa gccccaggta gacgggaatt ttttctctgc
    15061 cgatccggct gagaaatatc tctttcctat attcagcgcc ggattccgtt agttttggca
    15121 ggcttgcaaa ctttgaaaaa ttgccggcaa gataattccc gagtttctgc aaaccattaa
    15181 aatggttcat taacagcttg ttgtccgagg cgtacatcac agctccgatg gctcccatcg
    15241 aagcgtagta cgggggaatg accagcccgt cgtcggcgag ttccagcact tcgcggaaag
    15301 ccctgaccat cccggcattt gccgctaccc cgccggagaa tatcaccggt tttataatct
    15361 ctttgctccg ggcgacattg ctgcgaaaat tccgcgccag ggcaaaacac aatccggcga
    15421 caatatcatg caacggcgtt gcaatctgct ggtgatggat catatcgctc ttggcgaaaa
    15481 cgctgcacct accggcaata cgcggcggat cgacggactt cagcgccatt tcgccgaatt
    15541 cattctcgat ggaaacgcct atcctgcgcg cctgctgatc aaggaaagaa ccggtgccgg
    15601 cggcgcagat gctgttcatt tcgaaatcgg ccagacggga ttgtccgctt ttcggatctt
    15661 tttcaagcag gataagtttt gaatcttcac cgcctatctc aataacggtg cgggcttcgg
    15721 ggaacagctt cccggtggaa gcggcctggg caatgatttc gttgacgaat accccgcccg
    15781 tcagctcagc cgccagtttt cccccggatc ctgttatggc gatacctttg atggattcaa
    15841 caggatgatg ctgcaataaa ccattcagcc tgtccctgaa aacctggaaa ggccggccgt
    15901 gaacccagtc ataataattc tcagtcacgt tgaaacgcac atccatcacg acggcgttaa
    15961 gggaaaccga cccaaggtcg aagcctatca gaacggcttc agcagtatgt ctgccatcgg
    16021 gaaacccagt atccataaag tgattccaac aggttgatcc gatattgata aagagtatgt
    16081 tattgtaatg ctaatatacg atatgttttc tcattaatca atataaatat ctgacgatct
    16141 ccctaatgct gaaatttctg atttcttttt cttcccatgg gaaaatcttt cacctgaacg
    16201 atcaggattc cggtcagcat aagcagacac ccggcaaggt tgcgaaaggt catggattcg
    16261 tgcagtatca gccagccgcc gattacagca aacacagttt cgaaactcag gatgatggag
    16321 gcataggcgg gatgaacata cttttgggcc accacctgca gggtataggc aacgcccacc
    16381 gacagcagtc cgccgtaaag caggggaaca gacgctttaa aaatatcgcc gggcagggta
    16441 gtttcgaaga aggatgaaac ggccaggctc agtatcccgc atatggcaaa ttgcatagcc
    16501 gacaacggca gggccggcag ttttttcacc aggctgttga tcagttgcac atgtaccgcc
    16561 caaaagaaag cgctgaccag gaccagaaga tcgccttttt cgatgatcaa cggtccgcgg
    16621 aaggccagga gaaaaagacc tgctaccgcc acggcagcgc cgatccatag gttacgcccg
    16681 gttatctgcc cgaggaaaat accgatcgca ggaacgatga gaacatataa accggtgata
    16741 aacccggctt ttcctgcctg ggtgtaaaca ataccggcct gttgcagcga tgcggcgacg
    16801 aaaaggacga ttcccgccgc gaggccaccc ctgaataaac cgggggatat taacaaaacc
    16861 ttcttttttt ctctgtggtt taaccagaaa ataagcggga gcaacgacaa acttcccagg
    16921 aggaatctca gtccgttgaa aatgaacggc ccgacatgca ccatgccggc acgctgggcc
    16981 acaaaggcaa atccccagat ggcagccgtg agccataaca tggctattgc tttggggcgc
    17041 tgagatactt tcaatgtcgt ttgtaatttt ctaagtgcta tatttgccgt ttatcctaac
    17101 cttattcaaa cgttccacgc catgaaatta cttcttttaa gcaacagtac gaatgccggc
    17161 gaagaatacc tgggttggcc ccgaaagctg atcatcgatt ttttgcagga ttacaatatc
    17221 aaaaccgcag tttttattcc ttacgccggt gtaaccatca acaacaacga ttatgaagcc
    17281 agggttgacg aggtatttgg cccgatggga tgtaagatca catcggtcca caaggtaaat
    17341 gacccggtgg aagccgtaaa aagcgcagaa tgcatcatcg ttggaggtgg aaataccttc
    17401 aggctggtgc aactgatgta tgacaaaaaa ctgatggaac ccatccggca gcgggtgatg
    17461 gaaggcgttc ctttcatcgg atggagcgcc ggttccaatg tcgcctgccc gagcatgaag
    17521 acgaccaatg atatgcccat tgtacagccg ttaagccttg ataccctgaa tctggtgcct
    17581 ttccagatca atcctcatta cctggattca aatcccgagg gccacggcgg tgaaacccgc
    17641 gagcagcgta ttatcgaatt tctcgaagtg aaccgtaaaa taacggtcgt cggcctgagg
    17701 gaagcctgtg ctctccggta catggattcc cgtttgcatc tgctgggaaa ccgccccatg
    17761 cgcgtcttca ggtatcatac ggttccggtg gaatatgaac ccggaagcga tatcgacttc
    17821 ctgctcagcc cggcgatata atccggaata aaaaaccggc attgttcaat gccggttttt
    17881 ttaatgcttc agtttcagaa ggtttctcag aaagtgtacc tgacagagag ctggctttgc
    17941 caccaccacc agtgatgcac ctcatcccat tcatttccga ttacgttaac ggcaggacga
    18001 acgtcgagcg agaaggcgac cggaatatca tgaggtttta attcgaggcc gatgacgccg
    18061 tcgccgccca ggaaggcgcc ttccccgtaa ttaccgccgt accagcttcc gatatgggcg
    18121 ccaatgccat aataccattt aaaatcatcg gagagcggtg catgaatttc atataagccg
    18181 gtaacaacat aataatggct gaaaccgccg acggtgaaat cgagcgcttt actttcgttg
    18241 aggaatgttt taaaacttat tcccgaaggc gatccgaaag caagtccaag gctgttttta
    18301 tagtcctgag catttgctgt aaaaatgacg atggagaaaa tagtaaccag gagcgttttt
    18361 ttcatgattt tgaaatttta aacggaaaaa gatattcaag aattatgcca gaatcccgtc
    18421 ttccgggatt tgaaccgggc ccggatggat attcttttat ccggagaatc ctgtcctgtt
    18481 cgtggtcaac ttattgtaaa gatacaatca tctgaaaaaa atctttcgct tttcgtattt
    18541 ttgtaataga gcagtcatat tggctgccgt ttctgtcaat caaccattca tccataaaaa
    18601 caatcagtca tgagcttcga attgcccgtt ttgccttacg gcacagatgc gctcgaacca
    18661 tacatcagcg ctaaaaccat tgaattccat tatggaaagc atcaccagac gtatgttaca
    18721 aaacttaacg gactgcttgc cggcagcgca tttgagaatg ccggtctcgg ggagattatc
    18781 cggaaagccg aagggcccgt ctataacaac ggcgcacagg tgtggaatca taccttctat
    18841 tgggaaggtt tttcatcccg gcggggctcg tcgccgcagg gaaatctgct ccggatgatt
    18901 gatcgcgatt tcggttcgtt cgaccagttc agggcgaaat ttaccgatgc cgccgtgaac
    18961 cttttcggat cgggatgggc ctggctggtg cagaaagccg acggttcgct ggccattatc
    19021 cagaccggca atgccgggaa tccgctgcgc gacggccaca aaccgctgct gacctgcgac
    19081 gtttgggaac acgcctatta ccttgattat cagaaccgcc gtcccgatta tgttgccgcc
    19141 ttctggaacc ttgtcgactg ggatgtggtg gctgccaggc tgggctaagg gaacaggaac
    19201 aaaatgaacc ggctgaaagg ccggtttttt atttgttgaa aatcagtgcc gggaaataaa
    19261 aaccggaaac cttaacctgg ataatcatgg ttacgaaact tccggcccgg tagccgcccc
    19321 gcgaataccg gtactttttt atgattgata aggatctatt ctacaatttg atgaattttt
    19381 cagtatatct tcctttgttg tcagtaacgg tcagtaaata aactcccggc agtaacggac
    19441 ttacattaag ttcaatgtaa ttattcgtta cttcaacagc tttattttca ataattcttc
    19501 cgtcaatgct ttgaattata gcacggcatt gattaccggg cactgaaccg gagaaaatac
    19561 cggttaacct gtcgcggaca ggattggtaa ggatgttaaa agcttttagt gtcgggtagc
    19621 tctttgacgc agaggtaata gttgatttgg cacttctata gagataaggt gatggcacag
    19681 aagtacatac atacatataa tcctcattgt ctgctactag ttcacccatc gctccccaag
    19741 gtaatccgct gtcgatttga gtaaaaatca gcccattatc aaatgatctg attatcccat
    19801 tcgggttacc aatccctgca taaatatctc ctgccgaatt gatggctatg ccgttcacct
    19861 ccgggccata caggcaggta tcaatgccgg ttgaatcgtg gtaaagcgcg tacaggccgc
    19921 cggcgccttg cagaaaatcg taatacaccc caatgaacac gtctccctgt tcgttgattt
    19981 ccactgcctg cacctggtgg tttatcaggc ccacctcctg ccaggtcgct ccctggtctg
    20041 tcgatttata aaccccgccc tggttgggga agaagcacat aaggccgaaa tatatatcgc
    20101 cattcggcgc aacggctatg tcggatattc tttctgccac atgagtagtt tccctgtaca
    20161 gtgtatcgaa agtcaaacag ccatcgggag tcctcagaag tgtggcccct ccggccacgc
    20221 atcctatcag cagagaatct cctcccaggc aataaaatct ggatatgcca ctggctgttg
    20281 accagtttaa taattgccac gaatcaccat tgtcagttga tttccaaagg ttatcaaagc
    20341 catttgaatg agcataaata tatccaaggc tgttaatggc aacagcattt atctgaaaag
    20401 tacctgtatt taaaacctgt tcccaggttt ggccggaatc agtagaacga taaacgccat
    20461 ttgaatatgt atccagcgtt ccgacaaaaa tatctccctg ggcattcacg gcaacatccc
    20521 cgatatcaac atccgggaac ggcagcaact cccagaaatc ctgtccctta gcatttaagg
    20581 caagcaacaa accggcaaat aaaaagagca atattttata tttcataagt caggctgttt
    20641 gtttgaggtg tggcagatat tataagcatt cgaatataat gaaatttact tgtacaaatt
    20701 cgtaaaatca tttccttcat ttgctgctgt tctaccatac tttctgttta tttcgtaggt
    20761 ataaatgtac caaacctccg acatctgcac acaactatta ccttcctgtg tcttgatata
    20821 agaaaagtat ggaagagtat cattatatat aaaatccggc aagggctttc catatgatgc
    20881 ggctgtagga atgagttcaa tataattcca atatgatgag gttgaagaaa caggcgccct
    20941 taatccgccc gatgcccaca ccggcccgtc cggatcggga ttcatgttaa ttaattgggc
    21001 aaaaacagga attctgaaca aggtca
//
LOCUS       DAOPYV010000056        20278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_2741_length_20278_cov_13.175839, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000056 DAOPYV010000000
VERSION     DAOPYV010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20278
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2741_length_20278_cov_13.175839"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PKI35_08250"
     CDS             <1..330
                     /locus_tag="PKI35_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44409.1"
                     /translation="QTENSGYNEYDLVVMKLNRDGTITGIDKPGEAPVNVTVYPNPGE
                     STVSVCGKVAGCLFSMYDNTGRLILQKKLTANNEMLDTADWPAGFYTYRVTGSGSFVA
                     AGKWIKL"
     gene            511..1521
                     /locus_tag="PKI35_08255"
     CDS             511..1521
                     /locus_tag="PKI35_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNX44410.1"
                     /translation="MKNTTRYLFPFFTVFILFCILMSCNNRHDRNNKAIPAEPAENIT
                     AAPFNADSAYEFIKKQAAFGPRVSNSDANRRCGKWLENTLKRFTAEVKVQSFESQAYN
                     GKSLKLNNIIASFNPQAEKRILLCSHWDSRPYADHDPDISNRRKPIDGANDGASGVAI
                     LLEIARQFSLKAPSAGIDIVLFDGEDYGAPEDEQSQVENDWCLGSQYWSKNPHLPAYS
                     ARYGILLDMVGAPNATFTREGTSVYYAADYVNRIWHVASQIGYSNYFVNDQTAAILDD
                     HVYINEIRQVPTLDIIHHDPSTQSGFCSTWHTLGDNLQGIDKQTLMAVGQTVITVLYL
                     EK"
     gene            complement(1531..2907)
                     /locus_tag="PKI35_08260"
     CDS             complement(1531..2907)
                     /locus_tag="PKI35_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNX44411.1"
                     /translation="MLNKPVVRPVKRVKVKDDKDKKIFVLDTSVILYNHNAINCFDDN
                     DVALPITVLEELDNFKKGNDTINFEAREFIRIIDRLSEKSTLQKWTTLTRAKGGGKFK
                     VIMNEQSEIDARKVYGDNKPDHRILNAALSLVKEYPERKVILVSKDINLRLKAKALNI
                     TAEDFLTGKIKDVEGLYTGKTTIEGLSSKVIDAMYQTGWVDPKEIGVEDPIPNHYFIL
                     RNTKNSALGFYNHATGRIERIDKRPAYRIVPRNAEQVFALHAIMNPDVKLVTLQGVAG
                     TGKTLLALAGAMEQRRDFKQIYLARPIVPLSNKEIGFLPGDVKEKINPYMEPLYDNLK
                     FIQNQFGDKDKETRVISDSLQNEKLVVTPLAYIRGRSISNVCFIVDEAQNLTPHEVKT
                     IITRAGEGTKIIFTGDIYQIDTPYLDAQSNGLSYLIDKIKHHPIYAHIRLEKGERSEL
                     ANLANDLL"
     gene            3311..4099
                     /locus_tag="PKI35_08265"
     CDS             3311..4099
                     /locus_tag="PKI35_08265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX44412.1"
                     /translation="METTYTLITGASGGIGYEMAKIAAGDHHNLVLVARTGEKLELLA
                     GKLRAANKIEVVTIVMDLAEKDGAGKLISLLKEEHLAVDTLVNNAGFGDSGDFAAASL
                     QKNLEMINLNISALTQLTHYCLQNMGKTGRGRILNVASTAAFMPGPGMAVYYASKAYV
                     LSFSEALAREVKAKGITVTTLCPGPTGTNFFIAAGLRNSFLNSLFPKAAAEKVAKAGY
                     HGMIKGKHLVVPGLINKLVISAPRFLPRVILRNSVYKIHSGRAT"
     gene            complement(4230..4583)
                     /gene="ssb"
                     /locus_tag="PKI35_08270"
     CDS             complement(4230..4583)
                     /gene="ssb"
                     /locus_tag="PKI35_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNX44413.1"
                     /translation="MNTLRNSVQLIGRLGQDPEIRTLENGAKVANFRFATDDSYNDKD
                     GNRKEQTEWHNIVAWGNLAERCEKYLKKGKEVVISGRLTYRNWEDKNNSKHTTAEIVV
                     NEMLFVGSKPEEESK"
     gene            complement(4726..5073)
                     /locus_tag="PKI35_08275"
     CDS             complement(4726..5073)
                     /locus_tag="PKI35_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44414.1"
                     /translation="MKKIILKSDWIFVANGLKFIIFKGFPGTGFNSADYKQNFFIIIN
                     DSKDRCSQIWKGLREDSYLQHIPEKKTLRDIFISSLLYQTENQISGCINSACHLCDKP
                     VRFQTILNRVIQT"
     gene            complement(5195..5530)
                     /gene="ssb"
                     /locus_tag="PKI35_08280"
     CDS             complement(5195..5530)
                     /gene="ssb"
                     /locus_tag="PKI35_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNX44415.1"
                     /translation="MNTLRNSVKLIGRLGQDPEIRNLEKGSKMARFSLATSDTFTDKQ
                     GNRKEQTQWHNLVVWGNLATVCEKYLKKGKEIAVDGRIAYRNWEDKNGSKHISADIVV
                     TDLIFIGGK"
     gene            complement(5580..5918)
                     /gene="ssb"
                     /locus_tag="PKI35_08285"
     CDS             complement(5580..5918)
                     /gene="ssb"
                     /locus_tag="PKI35_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNX44416.1"
                     /translation="MNSLKNTVHLIGRLGQDPEVRTLDGGRKVARLSLATGETYASKD
                     GNRRAAIQWHNLIVWGNLAGVCEKYLTKGREVAIAGKLTYRKWEDSQGLKHTNTEILV
                     DELLLVGNSK"
     gene            6159..8018
                     /locus_tag="PKI35_08290"
     CDS             6159..8018
                     /locus_tag="PKI35_08290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX44417.1"
                     /translation="MGQEITISAVIITFNEEKNIGRCLRSLDGIADEIIVVDSGSTDK
                     TVEICKRHEARVFDREFDGFSEQKNWAGEQATCHYILSIDADEVLSEPLKKSILKVKH
                     DCTHEAFSFNRRNYYCGKWIRFAGWYPDRKIRIWRKGTASWQGAVHETLTFSKPDIGW
                     LKGDLLHYSFSSLHEHLRTIRRYSGIRADEAGRSGKAMPGLLVSILKAGWKFIQAYLL
                     KAGFLSGGKGFTVCLNSAMRYLIQYSAFQKKRNVAEPGKAICFFNTTKAWGGGEKWHL
                     SSANYIAGHSEKVYFAVSPGSALDRKITGTSLSKYRIKITNSSFINVFKLARLRRFFR
                     DNHIRTVIFNSPADLKAGGLAAFFAGVPNIIYRRGIAVSPRGNWFNTFLFSCVVDQFI
                     VNSHDTLKQLLKNVSIPADSLKINLIYNGLADPLAGESISPEIKDHADRKIILGNASR
                     LVKQKGLDHLLQVAALLNSRSIDFELRIAGTGPLQGELESAAAKLNLREKVKFLGFMD
                     RMDDFYKSLDIYLCTSEFEGFGYSIAEAMMAARPVVAFDTSSNPELIHDAETGYLVPA
                     FDTALFAGKVGLLIDDPSLRNRMGADARKFALMNFEINQQNVRLKNFLLNELK"
     gene            8042..9343
                     /locus_tag="PKI35_08295"
     CDS             8042..9343
                     /locus_tag="PKI35_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX44418.1"
                     /translation="MKKLALILMLLSFLPVLTKAQETPEQKAARMQWFADAKLGIFIH
                     YGIYAVNGISESWSFHNGQISYENYMKQLKGFTAKKYDPEAWAALIAESGARYTVLTS
                     KHHDGVALYDTKMNDLSVVKKTPAKRDLLTPFYKAIRDKGLKAGIYFSLLDWSHPDYP
                     NFLRDKKRYENDSLRWNKYLAFMNGQLDEIATRFNPDLWWFDGDWEFSADQWKAPQIR
                     EKLLKYNPNTIINSRLQGYGDYDTPENGVPIFKPKSPWWELCMTMNDSWGYEPTDINY
                     KTPNQIIRIFADCIGLGGNMLLDIGPKEDGTIPDEQVAILKEMGRWTSKHSEAIYGTV
                     AGVPREWFAGPSTLSKDRKKLYLFIDSKPIGPIVVKGLSNKVISAWVVGTGNPLNFNV
                     MMKPWWSPNPGLLYIDLPENLLDEQVTVICLSLETPIQEQP"
     gene            9429..9788
                     /locus_tag="PKI35_08300"
     CDS             9429..9788
                     /locus_tag="PKI35_08300"
                     /inference="COORDINATES: protein motif:HMM:NF021415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2116 family Zn-ribbon domain-containing
                     protein"
                     /protein_id="HNX44419.1"
                     /translation="MEQRHCLDCGEPIYGRLDKKFCSDQCRNNYNNRQNSDVTNYVRN
                     VNTILRKNRRILSELNPIDKVTVHKDKLVNKGFNFNYFTNIYTTQKGSSYRFVYEYGY
                     LPLDNDFYMIVIREQKK"
     gene            9830..10327
                     /locus_tag="PKI35_08305"
     CDS             9830..10327
                     /locus_tag="PKI35_08305"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HNX44420.1"
                     /translation="MPEDKNENTMTHIKRIALVAHDNCKKDLVEWVEWNWQLLKDHEL
                     ICTGTTGKLVAEAIAAKMEEGDAINLKLRRLKSGPFGGDQQLGALIAEGKIDVLIFFW
                     DPMEPQPHEVDIKALLRISVLYNIPSACNRATADFLISSHLLSEPYERSMPDYSAYLN
                     RKIQL"
     gene            complement(10342..11550)
                     /locus_tag="PKI35_08310"
     CDS             complement(10342..11550)
                     /locus_tag="PKI35_08310"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNX44421.1"
                     /translation="MRLQIFIPGILMVIIFASPSCRKAEIQPAKTLECQLEDASVYQV
                     NPKSKGFTEILDAYVAKGIPGIVLLVKDDNGFYIGSAGMADIDKGIKMQPCHISKIAS
                     ITKFMLGVAILRLQEKEVLSLDDPISKYLTETTLKHIKNGEEAITIRNLMNHTSGFYD
                     VIEDQGFYLQVLNDPAKHWEAEDLLNYVYDEPAMFPFAPEDTAAYSNTNYVLLSMIIE
                     KATGKSHSRILHEEVIDELGLQDTYYFYHDELPEGRVAQGYYDLYNNGTIVNLSNWNT
                     GSGNGYGGIYSTVWDMYLFADALFVKHTLLNEISLGQMLQFHHTVESRKHLGVACFKD
                     FIDIGEPRDYAWGHRGRDLSYSADLFYFPNHNAIMALMVNYGTDGDSFLKPVFLEMRD
                     EIAKLIVAPE"
     gene            complement(11587..12177)
                     /locus_tag="PKI35_08315"
     CDS             complement(11587..12177)
                     /locus_tag="PKI35_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44422.1"
                     /translation="MFRKLTFFILLAPFVVSGQILPYGPWSVCVTNNATMLPPASLTA
                     VLLQPVHPGVSVAYEFGWRKHEYYKWFQDAGISYSYHRFVSQAVVISTRAGYRRSLGA
                     LSFEASLLAGYMHMFLLTERAVQRSDGTWEPKKGFGKPQFVTGAGLGAGWDVGTEDTM
                     RRILINYDFRIQLPFVKNYVPLLPGGLLSLGFQFTL"
     gene            12359..15172
                     /gene="polA"
                     /locus_tag="PKI35_08320"
     CDS             12359..15172
                     /gene="polA"
                     /locus_tag="PKI35_08320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNX44423.1"
                     /translation="MKKLFLLDGMALVYQAFYAYKERPLINSKGLNTSAIQGFANVLY
                     KLLKEEKPTHIGVAFDTMAPTARHEGFIEYKANRQRMPEDLAASIPYIKRLIEAFNIP
                     TLSVEGYEADDVIGTLSKEAERAGYTVYMMTPDKDFGQLVSDKVLIYKPGKFGEKPVI
                     MGKSEVCEKFGIKDPLQVIDMLGIWGDASDNIPGIPGIGEKGAQKLLEKYGSLENILE
                     NLDSVEPESMRQKIINGRDLGIQSKQLATIILDVPIEFNEDNLHLDPPNPAALRELFE
                     ELEFRNFAKRVFTDLSLQEGSLPEDPRKERPQAKSASSVPDLFSELSDEDRGLADSSL
                     RTIGNTPHEYILADTTAKIDDLIKQLSTSLSFCFDTETTGLETATAELVGLSFALESY
                     KAWYVPVPEKRSEATVLLERFRPVFENEKIEKTGQNMKFDIEMLSGYGIRVKGRLFDT
                     MIAHYLLEPDMRHNMDLLSQNYLHYRPVSIETLIGKKGKGQITMRSVPLDQIKEYAAE
                     DADVTLQLREIFEPRLTQTETRKLFDEIEMPLIPVLAAMELEGVKIDTRALDEYSVEL
                     GKEIESLQQQIWEMAGTSFNIQSPKQLGEILYERLKITENVRKTATKQYSTAEDVLVK
                     LAHKHPIVQTVLDYRSLTKLKSTYVDVFPSLVNPKTGRVHTSYNQAVAATGRLSSNNP
                     NLQNIPVRTERGREIRKAFVPRNEEYTLLSADYSQIELRIIASISNDEGMMTAFKNGM
                     DIHTATAANIYGIRPEEVTRDQRRNAKSVNFGIIYGISAFGLSENIGISRKEAAEIID
                     QYFIKFPGIKTYMDNTIRFARENEYVETIMGRRRYIRDINSANAIMRGFAERNAINAP
                     IQGSAADMIKIAMIRVYDEFCSRNLRSRMILQVHDELVFDVCLDETEIVQTLVRECMQ
                     TAIPMNVPIEVEINTGANWLEAH"
     gene            15387..15797
                     /locus_tag="PKI35_08325"
     CDS             15387..15797
                     /locus_tag="PKI35_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNX44424.1"
                     /translation="MKQNGKILAGKNLKVTPQRLAILDAVNHLNHPSADEITEYIRKN
                     HPNIATGTIYKTLETFVQRGIIKKVKTESDIMKYDGVLSPHHHLYCTGSGRIEDYDDA
                     GLNTLLNDYFSHKNIPGFEIQDIKLQIIGRFTNA"
     gene            15855..16355
                     /locus_tag="PKI35_08330"
     CDS             15855..16355
                     /locus_tag="PKI35_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNX44425.1"
                     /translation="MMKEKSIELLNKAVADELLAVHQYMYFHFHLDDMGYDLLSNLFR
                     QTAIEEMMHVEKLAERILFLKGEVEMKASGDVKKINEPAEMLKLAVQMEQDSQNDYNK
                     WANECASNADAISKKLFELLVGEEEIHYDQYDVELDNLKKFGDKYLALQSIERSKNRI
                     TGTPAV"
     gene            16470..17573
                     /locus_tag="PKI35_08335"
     CDS             16470..17573
                     /locus_tag="PKI35_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNX44426.1"
                     /translation="MNIYHDNRIVMTLDAGGTNFVFSAAQAGEEIIEPIRLPAHGDTL
                     DQVLGTIVTGFSEVRKQLKINPAAISFAFPGPADYPNGIIGDLKNLPTFRGGVALGPM
                     LQHKFGIPTFINNDGDLFVYGEAIAGLLPQVNRELEEAGSPKRFKNLFGITLGTGFGG
                     GMVIDGKLFLGDNSAGAEIWAMRSKLHPHAYAEEGIAIRAVRRVYAEEAGIPFDEAPE
                     PKDICSIAKGQQPGNREAAIEAFRQLAVNAGDALANAITLLDALIVVGGGLSGAADLI
                     LPDLVAEMNNPLCNIAGEPLCRTELTAFNLEDISQKAKFLKGQAKTIPVPGTSLTVSY
                     DPLQRIGVGVSRLGTSRAVSIGAYAFALYKLDH"
     gene            17719..20019
                     /locus_tag="PKI35_08340"
     CDS             17719..20019
                     /locus_tag="PKI35_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNX44427.1"
                     /translation="MKKYSLLTLLIVLVITAFAQKTPWRPGAMELKINFKNPQEAAQL
                     GEMHLDADIYADYAYAYVVPDELALIKNAGFEYEILKPDLNAWSASFGEALVPPGYYT
                     FAQIKDIADSLATAYPAICKKVVFGQTATGLELAALKISDSVEADENEPEILFDGGIH
                     GDEVGGSQNMIYFARDLCKGYGTDTLLTNLINTREIWLYYCVNPYGRNQMTRENSNYV
                     DVNRDFGYMWGAEGSSPSPFSQLESKALRKCKLDNQFVVYTNYHSGTRMVSYPWSNRV
                     PAAPDKETINRLAQVYVSNSGYGAGQLPYGQGAIVMYFIQGATKDYDYGSLGSIGWSI
                     EISDDKQPPSSQIQYYYNMNKPAMLALIQHCGYGIQGTVTDAVTGQPVAAAVFEGNNY
                     PSYSDGVVGDYHRYLKPGTYTLKIAANGYETKTITDVTVNDLQCTTVDVQLQPIAGHY
                     AYRVLASHIPNLNGNNPGDELYSPACLMAPDQVSYSLGKNGYVIVDMQDSIINVEGDD
                     VIVYEGDASPESFTLYAGNTMDGPWISLGSGVGTTSFDLGKYNLSQARYYKIKDNSNG
                     TANVSDAGFEFDAIASLHPAIPDTVAHLNGTLYDGVTGLPIPNGIVSSGDSSCITDEN
                     GLYDLTLIRGNNIICADAIGYGLHCDTLALLPATITTHDFYLTSDVGINDNPESNDAL
                     LWPNPASDKATITFWLPAPQKVSIKVVNNVGKVCVKLAGQKFPAGENSCTINLAEQKN
                     VVAPGFYTVIIEGTAYHKCLKMLKCN"
BASE COUNT         5517 a         4923 c         4748 g         5090 t
ORIGIN      
        1 cagacagaaa acagcggtta taatgaatat gacctggtcg tgatgaaact caatagggat
       61 ggcactatca ccggcattga caagccaggg gaagcgcctg ttaatgtaac cgtctatccc
      121 aatccgggag aatcaaccgt aagtgtatgc ggcaaggtag ctggttgttt gttcagtatg
      181 tacgataata ccggaaggct gatcctgcag aaaaaactca cggcgaacaa cgaaatgctg
      241 gatacggctg actggcctgc cggtttttat acctatcgcg ttaccggttc cggttcgttt
      301 gttgctgccg gcaaatggat aaagctttga taagacagag tctgaaatag aggtccataa
      361 caacgaaagg ctgccggaat tgaatttccg gcagttttta ttaatggccg gttacagaac
      421 cacaacgcct gctgaatttg ttttcggcag aaaattgggt tagcatgcat ctttcctgta
      481 gttttgcatg gtttcaatcc aaaaaagaga atgaaaaaca caacccggta cttatttccc
      541 ttttttacag tttttatcct gttttgtatc cttatgtcct gtaacaaccg ccatgatcga
      601 aataacaaag ccattcccgc tgagccggcc gaaaacataa cagccgcccc tttcaatgcc
      661 gattcagcct atgagttcat caaaaaacag gcagcattcg gtccgagggt ttcaaattcc
      721 gatgccaacc ggcgttgcgg aaaatggctc gaaaacaccc tgaaacggtt caccgccgaa
      781 gtcaaagtac aatcgtttga atcgcaagcc tataacggga aatcacttaa actgaacaac
      841 atcatcgctt cctttaaccc gcaggccgaa aaaaggattc tgttatgcag tcattgggac
      901 tcccggcctt atgccgatca tgatcctgat atttccaacc gccgtaaacc gatcgacggc
      961 gccaacgacg gcgccagcgg agtagccatt ttactcgaaa tcgcccggca attctcgctg
     1021 aaagccccat cggccggaat cgacatcgta cttttcgacg gcgaagatta tggagcgccc
     1081 gaggatgaac aaagccaggt tgaaaatgac tggtgcctcg gatcgcaata ctggtccaaa
     1141 aatccgcacc tacccgccta ttctgcccgg tacgggattc tgcttgacat ggtcggagcg
     1201 ccgaatgcga cctttacccg tgaaggaacc tcggtgtatt atgccgccga ttacgtgaac
     1261 cgtatatggc atgtggcttc gcaaatcggt tacagcaatt attttgtcaa cgaccaaacc
     1321 gcggccatcc tcgatgatca tgtttacatc aacgaaataa ggcaggtacc caccctggac
     1381 atcatccacc atgacccgtc aacgcagagc gggttttgta gtacatggca cacactcggc
     1441 gacaaccttc agggaataga caaacaaacc ctgatggccg tggggcaaac cgtgattaca
     1501 gtcttgtacc tcgaaaagta ggaaaataaa ttaaagcaga tcattggcca ggttggccag
     1561 ttccgaacgc tccccttttt caagacggat atgggcgtaa atcggatgat gcttgatctt
     1621 gtcgatcagg tacgaaaggc cgttactctg ggcatcgagg taaggggtat caatctggta
     1681 aatgtcgccc gtaaaaatga tcttggtgcc ttctccggca cgggtgataa ttgttttcac
     1741 ctcgtgaggc gtgagattct gcgcttcatc cacgataaaa caaacattcg agatgcttcg
     1801 cccgcgtatg tatgcaagcg gtgtaaccac cagtttttcg ttctgcagcg agtcggaaat
     1861 aaccctggtt tccttgtcct tatcgccaaa ctggttctga ataaatttca ggttgtcgta
     1921 aagcggttcc atgtaaggat tgatcttttc cttcacatcg cccggcagaa agccgatctc
     1981 cttattgctg agcggaacga tggggcgggc gagatatatc tgtttgaaat cgcggcgctg
     2041 ttccattgcc ccggccaggg ccagcaaggt cttaccggta ccggcgacac cctggagggt
     2101 gaccagtttg acatcgggat tcatgatggc atgaagggca aaaacctgtt cggcattgcg
     2161 gggaacaata cggtaagcgg gacgtttatc gatgcgttcg atcctgccgg tggcatggtt
     2221 ataaaatcca agcgccgaat tcttcgtgtt gcggaggata aaataatggt ttgggatagg
     2281 atcttccacg ccgatttctt tgggatcgac ccagcctgtc tggtacatgg catcgatgac
     2341 tttcgaagac agcccttcga tggtggtttt gccggtatac agaccttcca cgtccttgat
     2401 tttcccggta aggaaatctt cggcggtgat attcaacgct ttggccttta gccgcaggtt
     2461 tatatctttc gagaccaata tcacttttcg ttcgggatat tctttgacca gtgataatgc
     2521 ggcattcaga atacggtgat ccggcttgtt atcaccatac accttgcggg catcaatttc
     2581 cgattgctcg ttcatgatca ccttgaactt gcctccgcct ttggcccggg ttagggtggt
     2641 ccatttctgc agggtgctct tttccgaaag gcggtcgatg atgcggatga attccctcgc
     2701 ctcgaaatta atggtatcgt tacccttttt gaagttatcg agctcttcga ggaccgttat
     2761 gggcaacgct acatcattgt cgtcaaaaca gttgatggcg ttatggttgt aaaggattac
     2821 gctggtatcg agcacgaaga ttttcttgtc cttatcatcc tttaccttga ctctctttac
     2881 aggacgcacg acaggtttat ttagcatacg cgttggtttt aaggcaccat tcataatgaa
     2941 gcggcttttc cgtattctaa aaatactgcc cgattttgcg gatttttact tcaggcataa
     3001 aatgatataa aaaagttgtt aacaaccgct tatccacaag gccaccgtgg ccagggtgca
     3061 ttgcatgaaa tattggcttt ttgtacgtct ccttcgcctc tgtcccctga catgtgtggt
     3121 gagattttat aatgcttaat aaaattgttt acttttattg catgattggc aaaattatat
     3181 ttctgaataa gtgacgaacg cttcagagga gcaagatcga aaataatctc aggcaaattg
     3241 aagatttctc gaacttcgca ccctgttgaa aaactgacag atcagaggaa ttttagcttc
     3301 agttctactt atggaaacta catataccct catcacagga gcttcgggag gaataggtta
     3361 tgaaatggcc aaaatagctg ccggcgatca tcataacctg gttctggttg cccgaacagg
     3421 tgaaaaactt gaattgctgg ccggcaaatt acgtgctgca aataaaatag aggtcgttac
     3481 catagttatg gaccttgcgg aaaaagatgg cgccggcaaa ttgatctcat tactgaagga
     3541 ggaacacctt gctgttgaca ccctggtcaa caatgcaggc ttcggcgatt ccggcgattt
     3601 tgccgctgcc agtcttcaga aaaaccttga aatgataaat ttgaacatca gtgcccttac
     3661 ccaactgacc cattattgtc tgcagaatat gggtaaaacc ggccggggaa gaattctgaa
     3721 cgtagcctca acagccgctt ttatgcccgg acccggcatg gcggtatatt atgcatccaa
     3781 ggcatatgta ctgtcgtttt cggaagccct ggccagggaa gtgaaagcaa agggcatcac
     3841 cgtgaccacg ctttgccccg ggcccaccgg cactaatttt ttcatagcag ccggactccg
     3901 gaactcgttt ctgaacagtt tatttccaaa agctgctgcc gaaaaagttg ctaaagccgg
     3961 ttatcatggc atgatcaaag ggaagcacct cgttgttccg gggcttataa acaaactggt
     4021 catttcagcg ccccggtttt taccgcgggt aatcctcagg aattctgtgt ataaaataca
     4081 cagcggccgt gcaacataaa ggcttcaaat gagcggcgtc ctcaaaggta atgactgaga
     4141 tataatcccc ggataaaaac aaccttttat cagcaagccg caacataaat tatatattgc
     4201 ggcttgacgg actatcagcg gttatccggt tacttgcttt cttcttccgg tttggaaccg
     4261 acaaaaagca tttcattgac cacaatttcg gctgtggtat gtttgctgtt gttcttgtct
     4321 tcccagttgc ggtaagtcag tcgtccggag ataacaactt ccttcccttt tttcaggtat
     4381 ttttcacaac gttcggcaag gttaccccag gctacaatgt tatgccactc ggtttgttcc
     4441 ttgcggttgc cgtctttatc gttataactg tcatcggtgg cgaaacggaa attggccact
     4501 ttggcgccgt tttcgagagt acggatttcg ggatcctggc caaggcggcc gatcagttgt
     4561 actgaattgc gtagtgtgtt catggttaaa aggattaagg ttagggttta atggtttctg
     4621 cggctggcag attaatttga ttgaagatat tttggcaagt gaatatttat ttccatcaac
     4681 tgctgataac ttaaagacgc ttttcctcag ctataaatga tgacattaag tctgaatcac
     4741 cctgttcagt atagtttgaa agcggacagg cttatcacaa agatggcaag cactattgat
     4801 gcatcctgaa atctgatttt ctgtttggta aagtaaagac gagataaaaa tatcacgcag
     4861 ggttttcttt tccggaatat gttgcaaata actatcctct cttaaacctt tccatatttg
     4921 gctgcaacga tctttgctgt cattaataat gatgaagaaa ttctgcttat aatctgctga
     4981 gttaaatccg gttccgggaa accccttaaa gataatgaat tttaggccgt tagcaacaaa
     5041 aatccaatcg ctctttaaaa ttattttctt catgtccctg gaaatttcgg atagctgtac
     5101 ggcacggcaa tcagggattc atgaatgccg atcttcaaag cctttgatct tcatcatccg
     5161 tggcctgttg atcggcattc atcattcccg ggaattactt cccacctata aagatcagat
     5221 cggttaccac aatgtcggca gaaatatgct tgctgccgtt tttatcttcc cagttgcggt
     5281 atgcaatgcg gccgtccacg gcgatttctt tgcccttttt caggtatttt tcgcagacgg
     5341 tggcaaggtt tccccacacc accaggttat gccactgggt ttgctccttg cggttgcctt
     5401 gcttatcggt aaaggtgtcg ctggttgcca gcgaaaagcg ggccattttg ctgccctttt
     5461 caagattacg gatctcagga tcctggccca gacggccgat cagttttacg gaattgcgga
     5521 gtgtgttcat gacgcggtgt attttaggtt ttaaattttg tccggagttc tgattaaatt
     5581 tatttgctgt ttccgaccag aagcagctca tcaacaagga tctcggtgtt ggtgtgtttc
     5641 agcccctggc tgtcttccca cttgcggtag gtgagtttcc cggcaatggc aacttcccgg
     5701 cccttggtca ggtatttttc gcatacgccg gcaaggttac cccatacgat aagattgtgc
     5761 cactgtatgg cagcgcggcg gtttccatct ttggatgcgt acgtttcacc cgtggcaagc
     5821 gagagacggg ctactttgcg gcctccgtcg agggtgcgta cttcaggatc ctggccaaga
     5881 cggccgatca ggtgaacagt gttcttgagt gagttcatgg gttttgagtt ttgggttatg
     5941 aattttcgtt cttattttca atccgttatc gacgattgac gagccaaaag tattacggct
     6001 ctgaaacctg attcggttat taagcatttg tatccggata aaagcattta ttgtcgtttg
     6061 ttgtcgtttt tttttctgaa tattaattat ttaagaattc atcggaataa tcatgccgga
     6121 ttgctacttt tgcagttaat aatccgcgat aattgaatat gggccaggaa atcacaattt
     6181 cggctgttat tatcaccttt aacgaagaaa aaaatattgg tcgatgcctt cgttccctgg
     6241 atggaattgc cgatgagatc attgttgttg attccgggtc gacagataaa accgttgaaa
     6301 tatgcaaaag gcatgaggcc cgtgttttcg atcgggaatt cgacggcttt tcggaacaga
     6361 aaaactgggc cggtgaacag gcaacctgcc attatatcct gagcatcgat gcggacgagg
     6421 tcctctccga acctttaaag aaatcaatcc tgaaagttaa gcatgattgt acgcacgaag
     6481 cattttcctt taaccggcgg aattactatt gcgggaaatg gatccgcttt gccggctggt
     6541 accccgaccg gaaaatccga atctggagaa aaggaactgc aagctggcag ggcgccgtgc
     6601 atgaaacctt gacattcagc aagccggaca tcggctggct gaaaggcgac ttattgcatt
     6661 attctttcag cagccttcac gaacatctga ggacaatccg ccgttattcc ggaatcaggg
     6721 ccgacgaagc cgggagaagc ggaaaggcaa tgccgggttt attggtttca atcctgaaag
     6781 ccggctggaa attcatccag gcatatctgc tgaaagccgg gttcctgagc ggcggcaaag
     6841 gatttacagt atgcctcaat tcggcgatgc ggtatctgat tcagtattcg gcctttcaga
     6901 aaaagcggaa tgtcgcggaa ccgggaaagg ccatttgctt tttcaacact accaaagcct
     6961 ggggtggcgg ggaaaaatgg cacttatcat ccgccaacta tattgccggg catagcgaaa
     7021 aggtatattt tgcggtatct cccggtagcg cccttgaccg gaaaataaca ggaacaagcc
     7081 tgtcgaaata ccggattaaa atcaccaatt ccagttttat caatgttttc aaactggccc
     7141 gcctccgccg gtttttcagg gacaaccaca tcaggaccgt tatctttaac agtcctgccg
     7201 acctcaaagc gggcggactg gcggcttttt ttgccggtgt cccgaatatc atttaccggc
     7261 gggggatagc tgtttctccc agggggaact ggttcaatac tttcctgttt tcctgtgtgg
     7321 tcgatcagtt tatcgtcaac tcgcacgata cgctgaaaca attgctgaaa aatgtaagta
     7381 tccctgctga cagtctgaaa attaacctga tttataatgg cctggccgat cccctggccg
     7441 gcgaaagcat ttctcctgaa ataaaggatc atgccgatcg taaaatcatc ctgggcaatg
     7501 cttcccgcct ggtgaaacaa aaagggctcg atcacctgct tcaggttgcc gctttgctga
     7561 acagccggag tattgatttt gaactgagga ttgccggtac aggaccttta cagggtgaac
     7621 tggaatctgc agcagcaaaa ctgaacctca gggagaaggt aaaattcctg ggcttcatgg
     7681 accggatgga tgatttttac aaatcgctgg atatttatct ctgcacttcc gaattcgaag
     7741 gtttcggata ctccattgcc gaagctatga tggcagccag gcccgtcgtc gcattcgata
     7801 ccagcagcaa tccggaactt atccacgacg ccgaaaccgg gtatctggtg ccggctttcg
     7861 atacagccct gtttgccgga aaggtcgggc tgctgatcga tgatccgtcg ctcaggaaca
     7921 gaatgggggc cgatgcccgt aaatttgccc tgatgaattt cgaaattaat cagcaaaatg
     7981 tacggcttaa aaatttcctt ctgaacgaat taaagtgatg atttccttaa agaattgcca
     8041 tatgaaaaaa cttgccctta ttctcatgct cctttcgttt ttaccggttt tgacaaaagc
     8101 ccaggaaaca ccggaacaaa aggcagcacg catgcaatgg tttgccgatg ccaaactcgg
     8161 gattttcatt cattacggta tttatgccgt aaatggcatc agcgaatcct ggtcttttca
     8221 caacgggcag atctcctatg aaaattatat gaaacagctc aaaggcttca ctgcgaaaaa
     8281 atacgatccc gaagcctggg ccgccctgat cgccgaaagc ggcgccagat acacggtact
     8341 caccagcaag catcatgatg gtgttgcctt gtatgataca aagatgaacg acctgagcgt
     8401 ggtgaagaaa acgcctgcaa aacgcgattt gctgacgccg ttttataaag ccatccggga
     8461 taaagggctg aaagcaggaa tctatttttc ccttctcgac tggtcgcacc ccgattatcc
     8521 gaatttcctt cgcgataaga agcggtatga aaatgacagc ctgcgctgga acaaatacct
     8581 ggcgttcatg aatggtcaac tggacgaaat tgccacgcgt ttcaatcccg atttgtggtg
     8641 gttcgacggc gactgggaat tcagcgccga tcaatggaaa gcgccgcaaa taagggagaa
     8701 actgttgaaa tataacccca acaccattat caattcaagg ctccaggggt atggcgatta
     8761 cgacaccccc gaaaacggcg ttcccatctt caaacccaaa tcgccctggt gggaactctg
     8821 catgaccatg aacgattcgt ggggttatga gccgaccgat atcaattaca aaacgcccaa
     8881 ccagataatc cgcatcttcg ccgattgcat cgggcttgga ggaaacatgc tgcttgatat
     8941 cggcccgaaa gaggacggca ccatacccga tgaacaggtt gccatcctga aagaaatggg
     9001 aaggtggaca tccaaacaca gcgaagctat ttacgggacc gttgccggtg ttccgcgcga
     9061 atggttcgcc gggccttcca ccctgtcgaa agaccgtaaa aagctctatc tctttatcga
     9121 cagtaaaccc atcgggccga tagtggtaaa aggattatca aacaaagtca tctcggcctg
     9181 ggtcgtcggc accgggaatc ccctgaattt caatgttatg atgaaaccct ggtggagccc
     9241 aaatcccgga ctgttgtata tcgatctccc cgaaaacctg ctcgacgaac aggttaccgt
     9301 catctgtctg agcctcgaaa ctccgatcca ggagcagcca taagagagat ttgcctgacg
     9361 gacaaaacgg gaagaccggc gttgtttaac ctgatgacca atatttattc aatattcatt
     9421 tctcaattat ggaacaacgt cattgcctcg attgcggcga acccatttac gggcgcctcg
     9481 ataaaaaatt ctgctccgac cagtgccgta acaattataa caaccggcag aattcggacg
     9541 ttaccaacta tgtacggaat gtgaatacca ttctccggaa aaaccgcagg atcctgagcg
     9601 aactgaatcc aatcgataag gttaccgtcc acaaagataa actggtaaac aaaggattca
     9661 atttcaatta cttcaccaat atctacacca cgcaaaaagg aagcagttac cggtttgttt
     9721 acgaatacgg ttacctgccg cttgacaacg atttttatat gatcgtgatc agggaacaga
     9781 aaaaataact ttataaagaa tacctgaaac aggtttttcc ggaaaaacca tgccggaaga
     9841 caaaaacgaa aacacaatga cccacatcaa aagaatagcc cttgtggcac atgataattg
     9901 caagaaagac cttgttgaat gggtggaatg gaactggcaa ctgctgaagg accatgagct
     9961 gatttgcacc ggcaccacag ggaaactcgt tgcagaagcc atcgcagcca aaatggaaga
    10021 aggcgatgcg ataaatctta aactccggag gcttaaatca ggtccttttg gcggcgacca
    10081 gcaacttggc gccttgattg ccgaagggaa aatagatgtc ctgatatttt tctgggaccc
    10141 gatggaacca cagccccacg aagtagacat caaagcgctt ttgcgcatct cggtgttgta
    10201 caatatccca tcggcctgca accgcgccac cgccgacttc ctcatatcct cccacctgct
    10261 gtcggagcca tacgaacgct ccatgccgga ttattcagcc tatcttaacc ggaaaatcca
    10321 gctttaaccc ccgaagcgct cttattcagg cgctacgatg agtttggcaa tttcgtcgcg
    10381 catttccagg aaaaccggct ttaaaaaact atcgccgtcg gtgccgtaat ttaccatcag
    10441 cgccataatg gcgttatgat tagggaaata gaacaaatcg gcgctgtaac tgaggtcgcg
    10501 gccacgatgc ccccaggcat aatccctcgg ttcgccgata tcgatgaagt ccttgaaaca
    10561 agccacccct aaatgcttgc gcgattcaac ggtatgatgg aactgaagca tctgtccgag
    10621 tgaaatttcg ttcagcaatg tatgttttac aaacagcgca tcggcgaaaa ggtacatatc
    10681 ccaaacggtg gagtaaatgc cgccataacc attgccactg cccgtattcc agttcgacag
    10741 gttgacaatc gtaccgttat tgtaaagatc gtaatatccc tgtgccaccc tgccttccgg
    10801 cagttcatca tgataaaaat aataggtatc ctgaaggccc agttcatcaa tcacctcttc
    10861 gtgaagaatc cggctgtgtg attttccggt ggctttctcg atgatcatac tcagtaaaac
    10921 atagttggta ttggaatagg cggcggtatc ctcaggagcg aagggaaaca tcgcaggctc
    10981 atcgtataca taatttaaaa ggtcttcggc ttcccagtgt ttggccggat cgttcagcac
    11041 ctggaggtaa aatccctggt cctcgataac gtcgtaaaag ccggaggtgt gattcatcaa
    11101 attccggatg gtgatcgctt cctctccgtt tttaatgtgt ttgagcgttg tttccgtaag
    11161 atatttactg atgggatcat ccagcgaaag cacctccttc tcctgtaacc tcaggatggc
    11221 aacgcccagc ataaatttgg taatgctggc aattttggaa atatggcagg gctgcatttt
    11281 aatgcccttg tcgatatcgg ccattcctgc cgacccgatg tagaaaccgt tatcatcctt
    11341 taccaggaga acgatccccg gaatcccttt cgccacatag gcatcaagga tttcagtgaa
    11401 acctttgctc ttggggttta cctgatagac tgaagcgtct tcaagctgac attccaatgt
    11461 tttggccggc tgtatttcgg ctttccggca ggagggtgaa gcgaaaatta tcaccatcag
    11521 gataccgggt atgaatattt gtagccgcat ttcctaagtt tttaatgaga tccgacaacc
    11581 tgacttttac agagtaaact gaaatcccag tgaaagcaaa ccgccgggaa gcaaagggac
    11641 ataatttttc acgaaaggca gttggatccg gaaatcatag ttgatcagga tgcggcgcat
    11701 ggtatcttca gtgcctacgt cccatcctgc gcccagcccc gcgccggtca caaattgcgg
    11761 tttcccgaat ccctttttcg gctcccaggt accgtccgaa cgctgaacag cccgctccgt
    11821 cagcaggaac atgtgcatat aaccggccag cagcgaagct tcaaacgaaa gggccccgag
    11881 ggaacgccgg taccctgccc gcgtcgatat aaccactgct tgcgaaacga aacggtggta
    11941 agaatagcta atccctgcgt cctgaaacca cttataatac tcatgtttgc gccagccgaa
    12001 ctcataggcc accgataccc cgggatgaac cggctgcagt aacacagccg tcagcgacgc
    12061 cggcggcaac atggtagcgt tgttggttac gcataccgac cacgggccgt aaggtaatat
    12121 ttgtcccgac acaacgaaag gcgccagcaa tatgaagaag gtcagtttcc tgaacataaa
    12181 cccgattttt agtatcacat aaagatacgg aattcaccga gcgttaacaa gatatttttt
    12241 tcaatactgg cgtcttgcgt ctgcgcaaca ggttccattc ctacggtaga acaagcgccc
    12301 atcgttttat ccgttaaaat tgttaccttt agttcctgat aaatcaaata caattcactt
    12361 gaaaaaatta ttcctcctcg atggcatggc gcttgtttat caggcgtttt atgcttataa
    12421 agaacgtccg ctcatcaatt ccaaaggact gaatacttcc gctatccagg gttttgccaa
    12481 cgttctgtat aaattgctga aggaggagaa acctacgcat attggcgtgg cattcgacac
    12541 aatggcgcct accgcccgcc acgaaggttt tatcgaatac aaagccaacc gtcagcgcat
    12601 gcctgaggac ctggctgcat caattcctta tataaaaagg ctgatcgaag cgtttaacat
    12661 tccaaccctt tcggttgaag gatatgaagc ggatgatgtg ataggaacct tgtccaaaga
    12721 ggccgaacgg gccggatata ccgtttacat gatgaccccc gacaaggatt tcggtcaact
    12781 ggttagcgat aaggtgctga tttacaaacc ggggaaattt ggtgaaaagc ctgtgatcat
    12841 gggcaagtcc gaagtctgcg aaaagttcgg catcaaagac cctctccagg ttatcgacat
    12901 gctgggcatc tggggtgatg cctccgataa catccccggc attccgggaa tcggtgagaa
    12961 aggtgcgcag aaactactcg aaaaatacgg ctcactcgaa aatatcctgg aaaatctcga
    13021 ctcggttgaa ccggaaagta tgcgccagaa aattataaac gggcgtgacc tgggaataca
    13081 gtcgaaacaa ctggcaacca ttatcctgga tgtgcctatc gaattcaacg aggataacct
    13141 ccacctcgat ccgccaaatc ccgctgcact cagggaactt tttgaagaac tcgagttcag
    13201 aaattttgcc aaacgggttt ttaccgacct ctcgttacag gaaggatcct tgcccgaaga
    13261 tccgcgtaaa gaacgtccgc aggcaaaaag cgcatcatcc gttcccgatc tcttttcgga
    13321 actgtccgac gaagaccgcg ggctggccga cagcagcctc cgaaccattg gtaatacgcc
    13381 ccacgagtat atccttgccg atacaaccgc gaaaatcgac gatctgataa agcagttatc
    13441 cacatccctt tccttctgct tcgatactga gacgaccgga ctggaaaccg ccacagccga
    13501 actggtggga ctttccttcg ccctggaatc ttacaaagcc tggtatgtgc cggttccgga
    13561 aaaacggtct gaagcaacgg tactgcttga aaggttccgc cctgtttttg aaaatgagaa
    13621 gatcgaaaag accgggcaaa acatgaagtt cgatatcgag atgctttccg gctatggcat
    13681 cagggtaaaa ggcaggcttt tcgacaccat gatcgcgcat tacctccttg aacccgatat
    13741 gcgccataac atggacctgc tgtcgcaaaa ttacctgcat taccggccgg tgagcatcga
    13801 aacgctgatc ggtaaaaaag gaaaaggcca gattaccatg cgttcggtcc cgctggatca
    13861 gatcaaggaa tatgctgccg aggatgccga tgtaacgctg caattacgtg aaatttttga
    13921 gccccggctg acgcaaaccg aaacaagaaa actcttcgat gaaattgaaa tgcccctgat
    13981 accagtgctg gcagccatgg aactcgaagg ggtaaaaatc gacaccaggg ccctggatga
    14041 atattcggtt gagctgggaa aggagattga aagcttgcag cagcaaatct gggaaatggc
    14101 cggcacttcc tttaacatcc agtcgccgaa acaactgggc gaaattcttt acgaaaggtt
    14161 gaaaatcacc gaaaacgtca ggaaaactgc taccaaacaa tattccacgg ccgaagatgt
    14221 actggtaaaa ctggcccaca agcacccgat cgttcaaacc gtcctcgatt accgatccct
    14281 gactaagctc aaatcaacct atgtggatgt tttcccctcg ctggtcaatc cgaagacagg
    14341 cagggtacat accagttaca atcaggcggt ggcagcaacg ggacggttaa gttcaaacaa
    14401 cccgaacctt cagaatatac cggtccgcac cgagcggggg cgtgagatac gtaaggcatt
    14461 cgtcccccga aacgaagaat acacgctgct gtcggccgac tattcccaga tcgaacttcg
    14521 cattattgcc tccatcagca acgacgaagg catgatgacc gcctttaaaa acggcatgga
    14581 cattcatacc gccacggcag ccaatatata cgggatcagg cccgaagagg tcacccgcga
    14641 ccagcgccgt aacgccaaaa gcgtcaactt cgggattatt tacggtattt cagccttcgg
    14701 gctgagcgaa aacatcggca tctcgcgcaa ggaagccgca gaaatcatcg accagtattt
    14761 tatcaagttc ccgggaataa agacatacat ggataatacc atccgctttg cccgggaaaa
    14821 tgaatacgtg gaaaccatca tgggacgacg aaggtatatc cgggacatca attcggctaa
    14881 cgccatcatg cgcggcttcg ccgaacgaaa tgccatcaac gcacccatcc agggttcggc
    14941 tgccgatatg attaaaatcg ccatgatccg tgtgtacgat gaattctgta gccgtaacct
    15001 ccgcagccgc atgatcctgc aggttcatga cgaacttgtg tttgatgttt gccttgatga
    15061 aacggaaata gtacaaaccc tcgttcgcga atgcatgcaa acagccatcc ccatgaatgt
    15121 gccgattgaa gtggaaatca atacgggggc aaactggctg gaggcgcatt gatcggaaaa
    15181 taaaatttga agggacggaa cctgccgggt gaaaagcagg agctgattca gttttaaaat
    15241 ggctggcgtt cattctgttg ttccttttcg ggtagctgcg actgccggtt tcaacatgtt
    15301 aaattttcct gtacctcttg ctttcccctg gaaaaaagtt taactttgtt ccttcttaag
    15361 aattgttcct atttaggatt gaataaatga aacaaaacgg gaagatctta gccgggaaaa
    15421 acctgaaagt aacgccgcaa cggctggcca tactcgatgc agtaaaccat ctgaaccatc
    15481 cttcggccga tgaaataacg gaatatatcc ggaagaacca cccgaatata gcgaccggca
    15541 ccatctataa aacgcttgaa accttcgttc agaggggaat catcaaaaag gtgaaaaccg
    15601 aaagcgacat catgaagtac gatggagtcc tcagccctca ccatcacctg tattgtaccg
    15661 gttccggccg tattgaagac tatgacgacg ccgggctcaa tactttgctc aacgattatt
    15721 tcagtcacaa aaacattccg ggtttcgaaa tccaggatat taaactgcag attatcggca
    15781 ggttcaccaa tgcctgaaat aatcacactg caaaaaaagc caaaaattca aataataaac
    15841 cactaaaacc aataatgatg aaagaaaaaa gcattgaatt gttaaacaag gctgttgccg
    15901 atgaactgct ggccgtacat cagtacatgt actttcattt ccatctcgac gacatgggct
    15961 acgatctttt atcaaatctt tttcgccaaa ccgccatcga ggaaatgatg catgttgaaa
    16021 aactggccga acgtatcctt ttcctgaaag gcgaagtgga gatgaaagca tcaggcgatg
    16081 tgaaaaaaat aaatgaaccg gccgaaatgc ttaagttggc cgtccagatg gaacaggaca
    16141 gccagaacga ttataacaaa tgggccaacg aatgcgccag caatgccgac gccatttcca
    16201 aaaaactgtt cgaactcctg gtcggagagg aagaaatcca ttacgaccaa tatgatgtgg
    16261 aactggacaa tctgaagaag tttggcgaca agtaccttgc cctgcaatcc atcgaacgca
    16321 gtaaaaaccg catcaccgga acaccggcag tataacatta cggcgattca acaaaaggcc
    16381 tggcatgttt cggaaacctg tcgggccttt tttttgtcgg ttcccctaaa attgtaattt
    16441 tggacgtttc acaggaaata ccccaagata tgaatatata ccatgacaac cgcattgtaa
    16501 tgaccctgga tgccggcggc accaattttg ttttttcggc agcccaggca ggcgaagaaa
    16561 tcattgagcc cattcggctt ccggctcatg gcgacaccct cgaccaggtt ctgggaacta
    16621 tcgtaaccgg cttcagcgaa gtgaggaagc agcttaagat caatcccgct gccatcagct
    16681 ttgccttccc ggggccggcc gattatccca acggcataat cggcgacctc aaaaacctgc
    16741 cgactttccg cggcggcgtt gccctggggc ctatgttgca acataaattc ggtattccca
    16801 ccttcatcaa caacgacggc gacctgtttg tatatggaga agccattgcc ggcctgttgc
    16861 cgcaggtaaa ccgcgaactc gaagaagcag gcagcccgaa gcggtttaaa aaccttttcg
    16921 gaataaccct cgggacaggt tttggaggcg gtatggtcat cgacgggaaa cttttcctgg
    16981 gtgataattc ggcaggggct gaaatctggg ccatgcgcag caaacttcac ccccatgctt
    17041 atgccgaaga gggtattgcc attcgtgccg tgagacgcgt atacgccgaa gaggccggca
    17101 tcccgtttga tgaagcgccc gaacccaaag acatctgttc cattgccaaa ggccagcaac
    17161 ctggtaacag ggaagccgcc atcgaagctt tccgccagtt ggcggtgaat gccggcgatg
    17221 cactggccaa tgccataacc ctgcttgatg cacttattgt ggtgggcgga ggcctttcag
    17281 gagctgccga cctcatcctg cccgaccttg tggccgaaat gaacaatccc ttgtgcaaca
    17341 ttgccggcga accgttatgc cggaccgaat taaccgcctt caacctcgag gatatttcgc
    17401 agaaagccaa attcctcaaa ggccaggcta aaaccatccc tgttccgggc actagcctta
    17461 ccgtcagtta tgatccgtta cagcggattg gcgtcggcgt ctcgcgactg ggcacttcgc
    17521 gggcggtttc catcggcgca tatgcttttg ccctttacaa actcgatcat taatttcata
    17581 caaatcagat aacctttgtg cgccggcgtt tatttacccg gcgcattttt tgtaataagt
    17641 gaaaatcagg cccgaacaca tttctaaaaa ttagtagttt tacaaccttc aaaattcact
    17701 catcctcatc tgcatgcaat gaaaaagtac tcactgttaa ccttactgat tgttcttgtc
    17761 ataactgctt ttgcacaaaa aacaccatgg cgccccggcg caatggaact gaaaatcaat
    17821 tttaaaaatc cgcaggaagc tgcgcagttg ggcgagatgc accttgatgc cgatatttat
    17881 gcggattacg catatgccta cgttgtaccc gatgaacttg cactgattaa aaatgccgga
    17941 tttgaatacg aaatcctgaa accggacctt aatgcatggt cagcttcgtt tggcgaagcc
    18001 ctggtaccgc ccggatatta cactttcgcc cagatcaagg atattgccga cagcctggcg
    18061 actgcatatc cggcaatctg caaaaaggtt gttttcggac aaacagctac aggactggaa
    18121 ttagctgcat tgaagataag cgacagtgtg gaggccgacg aaaatgaacc ggaaatactc
    18181 tttgatggcg gtatccacgg cgacgaagta ggcggctcac aaaatatgat ctactttgcc
    18241 cgcgatttat gcaaaggtta cggaacggat accttactta ccaacctgat aaacacccgg
    18301 gaaatctggc tttattattg cgtgaatccc tatggccgga accaaatgac ccgtgagaac
    18361 agcaattatg ttgatgttaa ccgtgatttt ggctatatgt ggggcgcaga aggatccagc
    18421 ccaagccctt tctcgcagct tgaatccaag gctttacgca aatgtaaatt agataaccaa
    18481 tttgtcgtat atacaaacta tcacagcgga accagaatgg tttcatatcc ctggagcaac
    18541 cgggtaccgg cagcgccgga taaggaaacg ataaatcgtc ttgcccaggt ttatgtgagc
    18601 aattccggat atggtgccgg tcagcttccc tatggccagg gcgccattgt gatgtatttt
    18661 atccagggcg ccacgaaaga ctatgattac gggagccttg gcagtattgg ctggtcaata
    18721 gaaatctctg atgataaaca accgcccagc agccagatac agtattatta caacatgaac
    18781 aaacctgcca tgcttgcgtt gatacagcat tgcggatacg gaattcaggg aacggttacc
    18841 gatgctgtta cagggcaacc ggtagcagcc gccgtttttg aaggtaacaa ttacccctca
    18901 tacagtgatg gcgttgtggg tgattaccac cggtatctga aaccgggaac ctataccctg
    18961 aaaattgctg ccaacggtta tgaaacaaaa accattaccg acgtcacagt taacgatttg
    19021 caatgtacta cggttgatgt acaattgcag ccgattgcag gacattatgc ttacagggta
    19081 ctggcatcac acatacccaa tttgaacggt aataatccgg gcgatgaatt gtacagccct
    19141 gcctgcctca tggctcccga ccaggtaagc tattcgctgg gcaaaaacgg ttatgtgatt
    19201 gttgatatgc aggatagcat tataaatgtt gaaggagatg atgttattgt gtatgaaggg
    19261 gacgcctcac ccgaaagttt cacgctctat gccggtaata ctatggacgg accctggatc
    19321 agtcttggct caggtgtagg cactacttct tttgatctgg gaaaatacaa cctctcgcaa
    19381 gcccggtatt ataaaattaa ggataacagc aacggcacag ccaatgtcag tgatgcgggt
    19441 tttgaatttg atgccattgc atccctgcat cccgccatcc ccgacaccgt cgcccatctg
    19501 aacggaaccc tgtatgacgg cgtaaccggc ttacctatac cgaatggcat cgtttcctct
    19561 ggcgacagca gttgcatcac tgacgaaaac ggactatacg atcttacatt aataaggggg
    19621 aataacatta tatgtgccga tgctattggt tacggtttac actgtgatac cctggccctg
    19681 cttccggcaa ctatcaccac ccatgatttt tacctgactt cagatgtcgg tatcaacgat
    19741 aatccggaaa gtaatgatgc ccttctctgg ccgaatccgg cttcggataa agcaaccatt
    19801 accttttggc ttccagctcc gcagaaagta agcattaagg ttgtcaacaa tgttggaaaa
    19861 gtatgtgtaa aactggccgg tcagaaattt cccgctggag aaaactcctg caccataaat
    19921 cttgcggaac aaaaaaatgt ggttgcaccg ggattttata cggtcattat cgaagggaca
    19981 gcttatcaca agtgcttaaa aatgctgaaa tgtaattagg caaatatgta atttttttag
    20041 tacaagtttg ctttcaggta gaaatcaaat actatcttta cgtccgtttt acaaattccc
    20101 taaaaaacaa cacaactaat tcctgtcacc ctatgaaaaa agtggtactc tctgttatta
    20161 tgttatgcct gggcacatcg ctgtttgcac aggtaatatt tacccaggat ttcagcagcg
    20221 gaaccatgcc tcctaccgga tggactattt tcgggaatac atccaactgg gttatatc
//
LOCUS       DAOPYV010000057        19788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_2852_length_19788_cov_12.163533, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000057 DAOPYV010000000
VERSION     DAOPYV010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19788
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2852_length_19788_cov_12.163533"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /gene="ilvE"
                     /locus_tag="PKI35_08345"
     CDS             <1..669
                     /gene="ilvE"
                     /locus_tag="PKI35_08345"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HNX44428.1"
                     /translation="TGASLYIRPLLFGSGKKVGVSPADEYMFLVFVTPVGPYFKEGFN
                     PVSIQIARDADRAAPMGTGRYKVGGNYAASLSSGERAHHEGFASALYLDAKEKKYIDE
                     AGPANFFGIKGNSYVTPKSDTILPSITNRSLMQLAESIGMKVEQRHVLVEELAEFDEV
                     GACGTAAVISPIKRIADRDNGHEFLYCKDGKAGAVSTKLYKLLLGIQYGDEPDKFGWI
                     TVVE"
     gene            complement(717..1856)
                     /locus_tag="PKI35_08350"
     CDS             complement(717..1856)
                     /locus_tag="PKI35_08350"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNX44429.1"
                     /translation="MKQRIFLIILVVLSLGWGSWLFLPKYMQQALLHLNPDITDYTIF
                     HNRTIVAGNPLPWPIHDGYNAESVSKSALLEFEKYGTVAFLVVKDGKLLHEEYWDGYN
                     DSTVSNSFSMAKSVISLLIGCLADEGKLSLSDPVVRYIPELKDDKDNPITIKHLLTMS
                     SGLDWDESYNNLTSVTTKSYYGSDITKLVTTLKKAEKPGATFRYKGCDSQLLGIIIER
                     VSGMPVSDYASLKLWRPLGAEKDALWSLDKKNGKEKTFCCFNATARDFARIGQLVLDS
                     GSFDGKQLVSKAYLQEATAPCSWLRDEDGKPCTYYGYQFWLTETQGHKVIYARGILGQ
                     YIFVIPSLNAVIVRLGKERSSEYVRHDTKDCFVYLNEGIRLARKP"
     gene            complement(1905..2750)
                     /gene="pyrF"
                     /locus_tag="PKI35_08355"
     CDS             complement(1905..2750)
                     /gene="pyrF"
                     /locus_tag="PKI35_08355"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNX44430.1"
                     /translation="MNRKQLFELIRQKRSFLCVGLDSDINKIPAHLLDSDDPVFEFNR
                     QIIEATIKFAVAYKPNFAFYESRGSKGLQSLEKTMLFLKKYENEVFTIADAKRGDIGN
                     TSAQYAKSVFDEPSSGFSFNAVTVAPYMGEDSVKPFLDFEGKWAIILALTSNKGSDDF
                     QLFSDTDGKRLFERVLERSQQWGNTNNLMYVVGATKAEMLGDIRKIVPGHFLLVPGVG
                     AQGGSLHEVAKYGLNDHCGLLVNASRSIIFASKGPDFAEKAKAEAAAMQEQMETLLRD
                     AGLID"
     assembly_gap    2923..3022
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3269..4354)
                     /gene="prfA"
                     /locus_tag="PKI35_08360"
     CDS             complement(3269..4354)
                     /gene="prfA"
                     /locus_tag="PKI35_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNX44431.1"
                     /translation="MAPITMLDKLEAINQRFLDLEKQLAEPDVMSDMKSFVKLNKDYK
                     ELQPIIAAYKDYRSLMNGIDTARTILNEEKDPELREFAKEELDTMLEKQGKMEEDIRL
                     MLIPADPQDSKNAVVEIRAGTGGDEASLFAGDLFRMYTRYCEAKKWKVEPVSVAEGTV
                     GGFKEVIFQVKGDGVYGLLKYESGVHRVQRVPATETQGRVHTSAATVAVLPEADEFDV
                     EINPSDIRKDTYCSSGPGGQSVNTTYSAVRLTHVPTGIVVAIQDEKSQIKNYEKAMTV
                     LRTRIFEMEYQKYLDDISKKRKTMVSTGDRSAKIRTYNYPQSRVTDHRINLTMYNLPV
                     FMDGEIQEMINELQLAENAERLKEAVE"
     gene            complement(4664..5596)
                     /locus_tag="PKI35_08365"
     CDS             complement(4664..5596)
                     /locus_tag="PKI35_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX44432.1"
                     /translation="MKNILVLLAFSMLALMLNAQPVELNTAKGTIYGTLQMPSTNGQV
                     PLALIIAGSGPTDRDGNNTSMKNNSLKMLADSLQVHGIASLRYDKRGIAASVGAGLDE
                     SELRFENYIDDAVAWVNLLKEENWFSKIYIIGHSEGSLIGMVAAAISPVDGFISLAGA
                     GFKSGDLLKTQLSGQPETVKNVCYSIIDSLETGKTVSNVDPMLYSLFRPSVQPYMISW
                     FKYNPCTEIQKLTIPVLIVQGTTDIQVSVDDAANLKKAKPDAAISIIEGMNHILKNAP
                     ADKQANLATYSDPVLPLNEDLIKSVTGFIKNFVN"
     gene            complement(5601..6767)
                     /locus_tag="PKI35_08370"
     CDS             complement(5601..6767)
                     /locus_tag="PKI35_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNX44433.1"
                     /translation="MPSDLKYNLRGVSASKEDVHNAIKNLDKGLYPNAFCKVIPDLLA
                     HDPEYCTVMHADGAGTKSSLAYIYWKETGDLSVWKGIAQDAVVMNTDDLLCVGITDGI
                     LLSSTIGRNKNRIPGEVIKAIIEGTEEFLEKMRSLGIGIYSTGGETADVGDLVKTVIV
                     DSTVTARAKRSEIIENRIQPGDVIVGLASYGQASYENGYNGGMGSNGLTSARHDVFSH
                     LYAEKYPESFDNSLPEEVVYCGKMMLTGATEVPGVDAGKLVLSPTRTYAPVISDILKH
                     HRGEIHGMIHCSGGAQTKVLHFIDGLHIIKDNLFPVPPLFRIIQDQSHTPWREMYQVF
                     NMGHRMELYVPEPVAEHIINISGSFSIEARIVGRCEAADGRSLTISTPDGIFNY"
     gene            complement(6779..9232)
                     /locus_tag="PKI35_08375"
     CDS             complement(6779..9232)
                     /locus_tag="PKI35_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX44434.1"
                     /translation="MKFKSLIFILLLTFSFGSVTAQNRAWRAAEDAFNSMQYNLALSK
                     YKKALSKARVKTDKEKISFQMAECYRIINNTKKAEPAYKRLATAKSGFADKNPLVYLY
                     LAQAMKSNEKYADAQKYFEKYAELVPEDPRGPNGASSCELAQEWLDNPTKIQIKNEKI
                     LNSKESDFSPTYADKLYNTIIFTSTREASTGKLQDNWTGQSFSDLYISKTDPKGAWST
                     PVLLDNGNVINTEANEGNPAVNSKFNQLYFTRCGNEKGTLQGCQIMMSRRAGRSYGDP
                     KPINLGGDSTTVFGNPGISPDELTLFFSSDLKGGQGGKDLWFATRKSPNDNFAKPQNL
                     GPVVNTPGDEMFPFMRNDTTLYFASNGHPGMGGLDIFRTTFSNGQWTTPVNLQSPVNS
                     SGDDFGLVYHPEEERGFFSSNRKGGKGSDDIYSFVNPPLIFTLQGTVKDDKTLQFVPA
                     AVVKLVGSDGSSVEARTDQKGYYSFSKSQVKPNTTYELICMKNNYFNKKAKETTVGLE
                     SSKDFTIDFKLEPIPDKPVVLPDILYDLAKWDLKPQYQDSLQGLIRTLDENETIIVEL
                     ASHTDNRGTDESNDILSQRRAESVVNYLIERGIDPDRLVAKGYGERVPRTLAKDTQKD
                     GFTFKAGTILTGAYIDSLVGKPMQEAAHALNRRTEFRVLSKDFVPKQQNKAITSQTQK
                     VSIQLNPEDNVVPYTENTDQTISFQAIVNGYTLSITLGETDGLTFSLEQALKLLKEGA
                     IGKTDFAGDPEKVLANGSIADKALFNVSEINIANKSAFDLEAQVLQKQKAGVLIDEST
                     LKKLAKYHLDKDKKQIVFE"
     gene            complement(9306..9713)
                     /locus_tag="PKI35_08380"
     CDS             complement(9306..9713)
                     /locus_tag="PKI35_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNX44435.1"
                     /translation="MTGHSILLFLDVSGGELLVIILVTFLVFGPKKMPEIARKIGKAM
                     NEMKRASNDISREFRNQTSDLVNEVNSVQNQVKREVSNTREAIRKNLDVGDPSDQPDA
                     EADPTSAVSHHEAPDITTPEPLPDVYRQSENAG"
     gene            complement(9875..12973)
                     /gene="secDF"
                     /locus_tag="PKI35_08385"
     CDS             complement(9875..12973)
                     /gene="secDF"
                     /locus_tag="PKI35_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HNX44436.1"
                     /translation="MQNKGAIRFFAIAFALVCLFQLSFTIVARITEKKAVRFAQTEAV
                     TAEAKQLAKGNAAREIDILDSLVQLRKKYFLDSVSNQPVYNLLIRKYTYKECLEHEIN
                     LGLDLKGGMNVTMEVSVSDVVKAMSGNSTNETFVKAMNMANEKMKNSQSDFVTLFGES
                     FTEIDPNAKLSAIFGRMELRDKIKPNSTNEEVLKVIRQECTDAFDRTFNILRNRIDRF
                     GVAQPNVQKLQTSDRILIELPGIKEPDRVRKLLQGTAQLEFWETYQFNVLSSYFTEAN
                     KRLASGNILDTLAAATDTIKAKKAADSTVAAKADTTKKTLAEKLKSDTTGKKKDNSFE
                     NYAKENPLFAYMRPAYYQGKNNQYYASDRATVGYAAIKDTARVNALLKKVKNIFPQNL
                     VLAWTAKPSPQMPDVLELTALKASTREGTAALDGSVVVDARQDYDQNGRVEVSMNMNS
                     EGAKKWKRLTADNIGKQVAIVLDGYVQSAPNVNDEIPNGRSSITGNYSIEEAQDLANV
                     LKAGKLPAPARIVQEEIVGPSLGTESINSGFLSFIIAFVLVLLYMQLYYNRAGLVANI
                     ALLTNVFFLVGVLASIGAVLTLPGIAGIVLTMGMAVDANVIIYERIREETRAGKGLRL
                     AINDGFKHAYSAIIDGQVTTIITGIVLYIFGSGPIQGFATTLIIGILTSLFSAIFISR
                     LVFERLLDRNIPIPFGNKYTINAFTNVNIDFIGIRKILYCVSGGIILIGIISLATKGL
                     NYGVDFAGGRSYIVRFDQNIKTNDVREALSKSFGEAPEVKTFGPSNQVKITTKYMVED
                     RTAVADSVVEHKMYDGLKGLYKAPVTFKDFSVANETIGILNSQKVDPTISDDIKKSSV
                     WAVTFALIAIFLYIFIRFRKWQYGLGGVISLTHDTLITISMYSIFYGILPFSLEVDQS
                     FIAAILTIIGYSINDSVIIFDRIREWTSLYPKRDLKTNMNGAMNSTLGRTFNTSGTTL
                     VTLIAIFIFGGDVIRGFIFALIVGIAIGTYSSIFNATPVAYDFIMIQKRRAEKKALAQ
                     KK"
     gene            13260..14129
                     /locus_tag="PKI35_08390"
     CDS             13260..14129
                     /locus_tag="PKI35_08390"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNX44437.1"
                     /translation="MTRQILFAAVLTVISGCLSAQQENFSSGDPVTVVTWNMRYNNPD
                     DGQNAWPNRKAAFLRILKDEKPDIFGLQEALYDQVQDVEKAFPRFKRVGVGREDGNTK
                     GEFAVIFYDTVKFRSLSSGNFWLSQTPFVPGSMGWDAACTRIVTWTQLQRKNGGAELF
                     VFNTHFDHIGQVARRNSAWLLLHAVDSLAGKRPAIITGDFNAQPGSEPVSILTGKNNP
                     IHFINSSDIAGVADSASFTYAGFKPGEIQGEIIDYIFIRNIRKVSEYSVNTCHDGDYY
                     PSDHLPVKAKVLF"
     gene            14144..16426
                     /locus_tag="PKI35_08395"
     CDS             14144..16426
                     /locus_tag="PKI35_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNX44438.1"
                     /translation="MLSVKYSWLIGLFILIPFTGKSQSSLKITQPVTAGPDVFDENGN
                     RLSMPFAGGLNACQLGAVDLNADGVEDLVVFDRQGYRLLTFINEGLAGEISYVYQPVY
                     EKYFPPVEHWMQLVDYNNDGKEDIFTYTTGGIKVYRNDSQDVPVFTQVSKPYLVSLQG
                     TTLTNIFVTYVDYPAIVDVDNDGDKDILTFWGLGSFVEWHKNLSMENFGTADSLIFEK
                     VSDCWGRFAEGAENNSIQLDTCVSFDEKMQAYSGLKGDDPKHTGSTLLVHDFNGDGLP
                     DLALGDVDYPALIRFTNGGTSEEAKMVSFTDSFPGDVNPVHITSFPACSAVDVNNDGR
                     TDLIASPFEPSLIRCDGFNSNWLYLDEGTGTIPGYNFIQKDFLQKDMIDLGSGAFPAL
                     FDYNGDGLQDLIVGNYGYVDSSWLDPVYGLKCEYVSSLALFENTGTADLPAFKLVTRD
                     YLNLSSLQMQSLIPAVGDVDGDHKTDILCGNSKGKFVFLKNKASSGQPADFEPADPAF
                     QQIDVGDFSAPQVADLDGDGLNDLVSGNKKGKLSYYRNTGQAGSPSYTLVTDSLGGID
                     VTNTAVSYYGYSIPLFYNLNDGQKMLLVGSEFGEIFVYSGIENNLDGHFTFEGLLQGL
                     EKGWRSSPALGNIDGDTLTELFVGNYSGGLTWYKGYHASPDGVGEERPAGKIRLEIIP
                     NPASDKATIMIEGLKEDPSGIRICLYDMSGARKYASERCGDRTEIPLNGFAPGLYPVV
                     VTLEIKGAVTQATGKIMVVR"
     gene            complement(16587..17096)
                     /locus_tag="PKI35_08400"
     CDS             complement(16587..17096)
                     /locus_tag="PKI35_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44439.1"
                     /translation="MKRLAIHNSGDAAYKKLTVMIFTEGTILGPRRLIDHFNHARYVP
                     IKNCVAKISNWDHDGARIVYLTSGRRESSAQSIRALLIKYGFPGSFLYYRSRGERYKD
                     IVETIKPDILVEDNCKSIGGRWQMSITYVDEKIKDKIKSVVVNEFQGIDLLPDRLSEL
                     PDYKPTGGK"
     gene            complement(17301..19583)
                     /locus_tag="PKI35_08405"
     CDS             complement(17301..19583)
                     /locus_tag="PKI35_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HNX44440.1"
                     /translation="MNKKLFLLLLPFLGIPAVFSQKITVKSGGVAYNVDIASGTASVG
                     QNYVKDPSSTAFTYKGTLTGDVVLAPEVKYKRKQYPVTTIEAGAFSGCSRITSITIPG
                     SVSNIEAGVFSYHLSDLKQITVSSENEYYKSADGALFSKDMTRMYRFPQGRNETGYTL
                     PSSVVSVEQRAFSFCSNLRIIVLPDHLNRIGDNAFEYCRFDSLAIPESVTEIGDHAFD
                     NCSNLTSVTIPEKVSKVGSQAFNYCYNLKSVICRNKDLIFAPDVFSNCSKLDGNIVYD
                     VDIRAVTEMARQGDPSYQYRLAMSYLNGEGLLKDQETARAWFEKSAAQGYVLSQAALG
                     DMYSKGLIPDKNHEEAIKWYTLAAGNGNVSAQSFLGDCYFNGIGVKQDPATAVRYYQQ
                     AAGQGDENACKQLGYCFYFGKGTGKDYQEAAKWYLKTAQKGDAESAYYMALMLYDGQG
                     MEKNDAEALNWAGKALDGGIYVCRWLYCELAYRDAVSLMDKGDYASAIDRFTSLLAYD
                     EKNTNAYINRGYCYLTRQPEDYVKAGADFRKALELDKDNRIALENMEVVNDHNKRVAE
                     AMTLCNEGFKCFSAKDYVNAVGNYAKAASLNNTDPAPYCCIGYCFFACELYSDALTFF
                     NKSLSVDPDYREAIEAIKNTKIIMLSKAIGDAAASFSNPLNSAYNNAANYGNSPAGSA
                     QPESDPYRNNATDKAVSRELQYGKDKYHTYYNLYEQEQAEADSYFSKYKAYGNLDDLK
                     KANNCQSRANEYLEKANIWK"
BASE COUNT         4719 a         4691 c         4870 g         5408 t
ORIGIN      
        1 accggcgctt cgctgtatat caggccgctg ctgttcggtt cagggaaaaa ggttggcgtt
       61 agcccggccg acgaatacat gttcctggtt ttcgttactc ctgttgggcc ttatttcaaa
      121 gaaggcttca acccggtaag tattcagatt gcacgcgatg ccgaccgtgc cgctccgatg
      181 ggaacaggcc gttacaaagt aggcggaaat tatgccgcca gcctctcttc aggcgagagg
      241 gcccatcatg aaggatttgc atcggctttg taccttgatg caaaagagaa aaagtacatt
      301 gacgaagcag ggccggccaa ctttttcgga attaaaggga acagttacgt cactcccaaa
      361 tcggatacca ttctaccgtc gatcaccaac aggagtctga tgcaactggc cgaaagtatc
      421 ggaatgaaag ttgaacagcg ccatgttctt gttgaagaac tggccgaatt tgatgaagtg
      481 ggcgcatgcg gcacagcagc cgttatctca cccatcaaac gtatcgccga tcgcgacaac
      541 ggacatgaat tcctttattg taaagatggg aaagcaggcg ctgtatcaac gaagctttac
      601 aagttgcttc ttggtattca atatggcgat gaaccggata aattcggatg gatcaccgtt
      661 gttgaataac atcaggtttt aaaatcaagc cggcgctgaa aagttgccgg ctttttttat
      721 ggcttccggg caagcctgat tccttcattc agatatacaa agcaatcttt ggtatcgtgg
      781 cgcacatatt cactgctgcg ttcttttccc agtcgtacaa tcaccgcgtt caacgaagga
      841 atgacgaaaa tatactggcc gagaatgccg cgcgcatata tgactttatg cccttgtgtt
      901 tccgttagcc agaattggta gccgtaatat gtacaaggct ttccatcctc atcacgcagc
      961 caggaacagg gagctgtcgc ttcctgaagg taagccttcg aaaccagttg cttcccgtca
     1021 aaactgccgc tgtccaggac cagttgacct atacgggcaa aatcccgggc cgttgcgtta
     1081 aaacagcaaa acgtcttttc ttttccgttt tttttatcaa ggctccacaa ggcatccttt
     1141 tcagcgccga gaggccgcca taatttgagt gatgcgtagt cggaaaccgg cattcctgaa
     1201 actctttcaa ttataatccc cagcagttgc gagtcacaac ccttatagcg aaaagtggcg
     1261 cccggcttct cagccttctt taatgttgtt acaagttttg taatatcact gccgtaatat
     1321 gattttgtag tcacagaggt caggttgttg taactttcat cccagtcgag cccggaactc
     1381 atggtgagca gatgttttat cgtaatggga ttatctttat cgtcctttaa ctcaggaata
     1441 tacctgacaa ctggatcgct gaggctgagc ttcccttcgt cagcaaggca gccgatcagg
     1501 agcgagataa cacttttcgc catcgagaat gagttgctga cggtggaatc attgtaccca
     1561 tcccaatatt cttcgtgcag cagttttccg tccttcacca cgaggaaagc aacggttcca
     1621 tatttttcga attcaagaag cgctgattta ctgactgatt ccgcgttgta accatcatga
     1681 attggccagg gcaaagggtt tccggctacg atggtccggt tatgaaaaat tgtgtaatcc
     1741 gtaatatcgg gattcaggtg aagcagtgcc tgctgcatgt attttggcaa aaagagccag
     1801 gagccccatc ccagtgatag aactaccaga atgataagga agattcgttg tttcatcggg
     1861 aagtatttaa agatttaccg cgcagcatca tgctgctcag tcaatcaatc tatcagcccg
     1921 gcatccctca gcaaggtttc catctgctct tgcattgctg cagcttcggc ctttgccttt
     1981 tcagcaaagt caggtccttt tgaagcgaag atgatactgc gggaagcatt cacaagtagt
     2041 ccgcaatggt cgttcagccc gtatttcgct acttcatgaa ggctgccgcc ctgggcgccg
     2101 acgcccggaa caagcaggaa atgaccggga actatttttc ggatatcgcc aagcatctca
     2161 gctttggtag ctccaaccac atacatgaga ttattagtgt ttccccattg ctgcgaacgt
     2221 tccaataccc gttcgaacag gcgttttccg tccgtgtcgc taaataactg gaaatcgtcg
     2281 gaacctttat tggaagtcag tgccagaatg atagcccatt tgccttcgaa atccagaaat
     2341 ggttttaccg agtcttcacc catataagga gctaccgtga cggcgttgaa cgaaaatcct
     2401 gatgatggct catcaaaaac cgattttgcg tattgggccg aagtattccc gatgtcgccc
     2461 cttttggcat cggcgatggt gaagacctcg ttttcgtatt ttttcaggaa cagcatggtc
     2521 ttctcaagac tctgcaaccc ttttgaacca cgactttcgt aaaaggcgaa gttcggttta
     2581 taggcaacgg caaattttat ggtagcctcg ataatctgac ggttaaactc aaaaaccggg
     2641 tcatccgaat ccagcaggtg ggccggtatt ttgttgatat cgctgtcgag tccaacgcag
     2701 agaaatgatc ttttctgcct gatgagttca aagagttgtt tccggttcat gggatgtaaa
     2761 atttgttatc cgcgaaaatt tattaacagc aacgttttcg cactaatatt ttacaaatat
     2821 acgattatct gctcgtggca cattcgtgta attcgtggat attgttactc cacgaattga
     2881 cacgaattaa acacgaataa gcccgaattg attttgcaga atnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nntccgggaa acgtttcgtg ataattcgtg ttccggcgca
     3061 gcctgtttcg tgaaatttgt ggttaagatt tcaccacgaa ttgacacgga ttgaacacga
     3121 ataagcacga attgattttg cagaatatcc gggaaacgtt tcgtgataat tcgtgttccg
     3181 gcgcagcctg tttcgtgaaa tttgtggtta aaaactccac ggaatgccca acagcagtat
     3241 ggaaaatact cccggaagta accgtaagtt actcaacggc ttctttcagc ctttcagcat
     3301 tttcagccag ttgcagttca ttgatcattt cctggatttc gccgtccatg aaaaccggca
     3361 ggttatacat ggtcaggttg atgcggtgat cggtaacgcg gctttgcggg tagttataag
     3421 tcctgatctt tgccgagcgg tcgccggtgg ataccatggt tttgcgtttc ttcgagatat
     3481 cgtcgagata tttctggtat tccatctcaa atatccgggt acgcaatacg gtcattgctt
     3541 tctcgtaatt cttgatctgc gatttttcat cctgaatggc aactacaatg ccggtaggaa
     3601 catgcgtcag cctcacggct gaataggtcg tattcacgct ttgtccgccg ggccctgatg
     3661 aacagtaagt gtctttccgg atatcgctgg gattgatttc gacgtcgaat tcgtcggctt
     3721 cgggcaacac ggcaaccgtt gcagcggatg tatgcacacg tccctgcgtt tcggtggccg
     3781 gcacacgctg cacacgatga acgccggatt cgtattttaa caggccgtaa accccgtctc
     3841 cttttacctg gaaaatcact tccttgaaac cgccaacggt tccttcggca acgcttacag
     3901 gctccacttt ccactttttt gcttcgcaat accgcgtata catacggaaa aggtcgcccg
     3961 caaacaggct ggcttcgtcg ccgccggtgc cggcacggat ttcaaccacg gcattcttgc
     4021 tgtcctgcgg atcggcaggg atgagcatca ggcggatatc ttcctccatt tttccctgtt
     4081 tctccagcat cgtatcaagt tcttccttgg caaactcgcg caattccggg tctttttcct
     4141 cgttcaggat ggtgcgggcg gtgtcgatac cgttcatcag cgatcggtaa tctttgtagg
     4201 cggcaatgat gggctgcagt tccttgtagt ccttgttcag cttcacaaat gacttcatat
     4261 ccgacatcac atccggctcg gccaactgct tctcaagatc cagaaagcgt tggtttatgg
     4321 cttcaagctt atctaacatg gtgataggtg caatggtttt tgcggataca aaggtaatgt
     4381 tttatagcga agcaggaaat gaattggctg acagtagcca aagggaagga tgcatcgaat
     4441 gcgttggtta tggtaagccg caaaatgact gatttattat tccgtgatca cggctacaaa
     4501 taatacttat aaacaataac ttttcatgat tatacaattc aggttatcag aggagcctgg
     4561 tttttcatcg atccactttc cgtgacggat tccgggctta atttttttaa tgattccctt
     4621 ttctgatgaa tcgggattat cctgatattc gcataattac aaactaattt acaaaatttt
     4681 taataaatcc ggttacactt tttataagat cttcatttaa cggcaatacc ggatcggaat
     4741 atgttgccag attggcctgc ttgtcagccg gggcgttttt caggatatgg ttcattcctt
     4801 cgataatgga aatggcggca tccggtttgg cttttttcag gttcgcggca tcatcaacgc
     4861 ttacctggat atcagtggtt ccctggacga tcagcacagg tattgtcagt ttctggatct
     4921 ctgtgcaggg attatatttg aaccacgaaa tcatatatgg ctgaacgctt gggcggaaca
     4981 gggaatataa catggggtca acattcgaaa cggtcttgcc ggtttccagg ctgtcgatga
     5041 tggagtagca gacgttttta acggtttcgg gctggcctga aagctgtgtt ttcaacaggt
     5101 cgccggattt gaacccggcg ccagccaggg aaataaaccc gtcgacaggt gatatggcag
     5161 cggcaaccat tccgattaag gaaccttcac tgtggccgat aatatagatt ttcgaaaacc
     5221 agttttcttc ttttaacagg ttaacccatg ccacggcatc gtcgatgtaa ttttcgaacc
     5281 gcagttcgga ttcgtcaagt ccggcgccta cgctggcggc aataccgcgt ttatcataac
     5341 gaagcgaggc gatgccatgc acctgcaatg agtcggccag catcttcagc gaattgttct
     5401 tcatcgaggt attgttcccg tcgcggtcgg tgggaccgga acctgcaata atcaaagcaa
     5461 gaggaacctg cccgttagtt gacggcattt gcagcgtgcc gtaaatggtt cctttagcgg
     5521 tattcagttc aacaggctgg gcgttcagca tcagcgccag catgctgaag gccaaaagga
     5581 ccaatatgtt tttcatgata tcagtaatta aaaataccgt caggagtgct gatcgtcagc
     5641 gaacggccgt cggcggcttc gcaacgtccg acgatgcgtg cttcgatgct gaacgatccg
     5701 gaaatgttga tgatatgttc tgcaaccggt tccggcacat acagttccat cctgtgtccc
     5761 atgttgaaaa cctggtacat ctcgcgccag ggcgtatggc tttgatcctg gataatcctg
     5821 aaaagaggag gaaccgggaa caggttatct ttaataatat gaaggccgtc aataaaatgc
     5881 aataccttgg tttgggcccc gccactgcaa tggatcatcc cgtggatttc accgcgatga
     5941 tgtttcagga tatcggaaat cacaggggca taggtacggg taggggagag aaccagtttg
     6001 ccggcatcca ccccgggtac ttcggttgct ccggtaagca tcatctttcc gcagtacaca
     6061 acctcttcag gcaggctgtt gtcgaaactt tccgggtatt tttctgcata tagatggctg
     6121 aaaacatcat gccgcgccga ggtgaggccg ttggagccca tgcccccgtt gtatccgttt
     6181 tcataggacg cctgcccgta actggccagc ccgacgataa catcgcccgg ctgaatccgg
     6241 ttttcgatta tttccgaccg ctttgcacgg gctgtaaccg tactgtcgac gatcaccgtt
     6301 tttaccaggt cgcctacgtc ggcagtttca ccccctgtgg aataaatgcc gatgccaagg
     6361 gaacgcattt tttcaagaaa ttcttcggtt ccttcgatga tggctttgat cacttcgccg
     6421 gggatccggt ttttattgcg tccgatggtc gacgatagaa gaatcccgtc ggtgataccc
     6481 acgcatagca ggtcatcggt gttcatgaca acggcatcct gcgcaatgcc tttccatacc
     6541 gacaaatctc cggtttcctt ccagtaaatg taagccaggc ttgattttgt accggcgccg
     6601 tcggcatgca tcacggtgca atactccgga tcatgggcaa gcagatcggg gattactttg
     6661 cagaaagcat tgggatataa ccctttatcc aggttcttga tggcgttgtg aacatcttcc
     6721 ttggaagcgc tcacgccgcg gagattgtat ttaaggtcgg aaggcacgcg ttgcagtatt
     6781 attcgaagac gatttgtttc ttatccttgt cgagatggta tttggctaac tttttcaggg
     6841 tggattcatc aatcagcacg ccggctttct gtttctgcaa tacctgtgct tcaagatcga
     6901 atgccgactt attggctatg ttgatctcgg agacgttgaa cagcgctttg tcggcgatac
     6961 tgccgttggc aagcactttc tcgggatcac ctgcgaaatc ggtcttcccg atagcgccct
     7021 ctttcagcaa tttcaacgcc tgttccagtg aaaaggtaag gccgtcggtt tcacccaggg
     7081 tgatggaaag cgtataaccg ttcacgattg cctggaacga aatggtctgg tcggtatttt
     7141 cagtgtatgg aacaacgttg tcttcaggat tcaactggat gctgactttc tgggtctggc
     7201 tggttatggc cttgttttgc tgtttcggga caaaatcctt gctcagcacg cggaattctg
     7261 tacgccggtt caacgcatgg gctgcttcct gcatcggctt cccgacaagg gaatcgatat
     7321 atgccccggt caggatagtg cctgctttaa aggtaaaccc gtctttctgg gtatctttgg
     7381 ccagcgttct gggaactctc tcgccgtatc cttttgcaac aagcctgtcg ggatcaatgc
     7441 cacgctctat aaggtaattg accaccgatt cggcgcgtcg ttgcgacagg atatcgttgc
     7501 tttcgtcggt tccgcggtta tcggtatgcg atgccagctc aacgatgatg gtttcgtttt
     7561 catcaagggt cctgatcagg ccctgcaagg aatcctggta ctgcggcttc agatcccatt
     7621 ttgccaggtc gtaaagaata tccggcaaca cgacaggctt gtcggggatt ggttccagct
     7681 tgaagtcgat ggtgaaatcc ttgctgcttt cgagccctac cgtcgtttct tttgctttct
     7741 tgttgaaata attatttttc atgcagataa gctcataggt ggtatttggc ttaacctgcg
     7801 atttgctgaa actgtaatac cctttctgat cggtgcgcgc ttcaaccgat gatccgtcgg
     7861 aaccgaccag ctttaccacg gctgccggca caaactgaag ggttttgtcg tctttcacgg
     7921 taccctgaag ggtgaaaatc aatggtggat tgacgaacga ataaatatcg tcgcttcctt
     7981 tacctccttt acggttcgaa gaaaaaaagc cgcgttcttc ctcgggatgg taaaccagcc
     8041 cgaaatcgtc accggaggaa ttaaccgggg attgcaggtt cacaggtgtc gtccactggc
     8101 cgttgctgaa ggtagtgcgg aaaatgtcga ggccgcccat accgggatgc ccgttcgatg
     8161 caaaatacag ggtggtatcg ttccgcataa aagggaacat ctcatcgccg ggagtattta
     8221 ccacaggacc aaggttttgt ggctttgcga agttgtcatt gggcgatttt cgtgtggcaa
     8281 accaaaggtc ttttcctccc tgaccgcctt tcaggtcgct ggaaaagaaa agggtaagtt
     8341 catccggact gatgccggga ttcccgaaga cggtggttga atcgcccccg agattaattg
     8401 gtttcggatc gccgtatgat cgtccggccc gccgtgacat catgatctgg catccctgga
     8461 gcgttccttt ttcgttgccg cagcgggtaa aatacaattg gttgaacttg ctgttcacag
     8521 ccgggttgcc ttcattggct tcggtattga tcacgttgcc gttgtccagc aataccggcg
     8581 ttgaccaggc ccctttcggg tcggtttttg agatgtacag gtcgctgaaa ctttgtccgg
     8641 tccagttgtc ctgaagctta ccggtagatg cttcacgggt ggaagtaaag atgatggtgt
     8701 tgtaaagttt atcggcgtag gtaggggaaa aatcggattc ttttgaattg aggattttct
     8761 cgttttttat ctggatcttg gtcgggttat caagccattc ctgggcaagc tcacaggatg
     8821 aagccccatt ggggccgcgt ggatcttcag gaaccaattc cgcgtatttt tcaaaatatt
     8881 tttgtgcatc ggcgtatttt tcattcgatt tcatagcctg agcgaggtac aggtacacga
     8941 gcggattttt atctgcaaat ccgctctttg ccgttgccag ccgcttatag gcaggttcgg
     9001 ctttcttggt attgttgatg atgcggtaac attcggccat ctggaacgaa attttttcct
     9061 tgtccgtttt caccctggct ttcgacaacg ctttcttgta cttggagagc gccaggttgt
     9121 actgcatact attaaaagca tcttcggctg cacgccaggc gcggttctgg gcagtgacgc
     9181 tcccaaaact gaatgtcagc aaaagaatga aaatgagaga tttaaatttc atgtatcttg
     9241 ttccttagag ggatgtcatc aattgataaa tttcgcaaaa tttaaacgaa tacgggaatt
     9301 atttattatc cggcattttc cgactgacga taaacatcgg gcaaaggctc cggggtggtt
     9361 atatcgggcg cttcgtgatg cgaaactgcg cttgtcggat cagcttctgc atccggctga
     9421 tcagatgggt cgccaacatc aaggtttttc ctgattgctt ccctggtgtt gctgacttcc
     9481 cgttttacct gattctgcac cgagttcact tcattcacaa gatccgatgt ttgattcctg
     9541 aattcacggg agatatcgtt ggatgctctt ttcatctcgt tcatagcttt gccgatcttc
     9601 cgggctattt caggcatttt cttgggcccg aacaccagga atgtcactag gattattacc
     9661 agcagttcgc cgccgctaac gtccaggaac aaaagtatgg agtgaccagt catcctgcta
     9721 ttcttattag atttacaaaa atactaaaca cctgcaatct gtcgacagtt tttatctttt
     9781 gacctgttca attctttccc gctataccca aactgcgata aaatgaaaac cccggcacag
     9841 ggccggggct ttcggatgtt ttggtaagct tgtgttattt tttctgagcc agcgctttct
     9901 tttcggcacg gcgtttctga atcatgataa agtcgtaagc aaccggggtt gcattgaaga
     9961 tggatgaata agtaccaatt gcaataccta cgatcagggc aaagataaat ccgcggatta
    10021 catcgccgcc gaaaatgaag atggcgataa gggtaaccag ggttgtaccg gatgtattga
    10081 aagtacgtcc cagcgtactg ttcatagcgc cgttcatatt cgtcttgagg tcgcgtttgg
    10141 ggtatagtga tgtccattca cggattcggt cgaagatgat taccgagtcg ttgatggagt
    10201 acccgatgat cgtcaggatg gccgctataa acgactggtc cacctctaga ctgaatggca
    10261 ggatgccgta gaagatggaa tacatcgata tggtgatcag ggtatcgtgg gtcagcgaaa
    10321 taaccccgcc aaggccatat tgccatttac ggaaacggat aaatatgtat aggaagattg
    10381 cgatcagggc aaatgtaacg gcccagacgg aagatttctt gatgtcgtcc gaaatggtcg
    10441 ggtcaacctt ctgagagttg agaataccga tggtttcgtt ggcaacggaa aaatctttga
    10501 atgtcacagg ggctttatat aatcccttta atccatcata cattttatgt tcgaccactg
    10561 aatcggcaac ggctgtacgg tcttcgacca tgtacttggt agtgattttc acctggttcg
    10621 atggcccgaa cgtctttact tcaggggctt cgccgaatga tttactcagc gcttcacgaa
    10681 catcgttggt tttaatgttt tggtcgaaac gaacgatata ggaacgtcct ccggcaaagt
    10741 caacgccata attcagccct ttggttgcca gtgaaataat accgatcagg atgataccgc
    10801 ctgatacgca gtacaggatt ttacggatgc cgataaagtc aatgtttaca ttggtgaagg
    10861 cgttgatggt gtacttgttg ccgaaaggaa tcggtatgtt cctgtcgaga agcctttcaa
    10921 ataccagcct tgaaataaag atggcggaga agagcgacgt gaggataccg atgataaggg
    10981 tggtagcaaa accctggatg ggtcctgaac cgaaaatgta caggacaata ccggtaatga
    11041 tggtggtaac ctgtccgtca atgattgctg agtaagcatg tttgaaacca tcgttgatgg
    11101 caagccgtaa tcctttaccg gctctcgttt cctccctgat acgttcatag atgataacgt
    11161 tggcgtcaac agccataccc atggtaagta cgataccggc aataccggga agcgtaagga
    11221 ctgcaccaat tgaagccaga acgccgacga ggaagaatac gttggtaagc agggcgatgt
    11281 tggcaaccag accggcacgg ttgtaatata actgcatgta taacagtacc agtacgaagg
    11341 caatgataaa ggataagaaa ccggagttga tggattcggt acctaaggaa gggccgacga
    11401 tttcttcctg gacgatgcgg gcgggagccg gcagtttacc ggctttcagc acgttggcaa
    11461 ggtcctgggc ttcttcgatg gaatagttcc cggtgatgga tgaacgtccg ttgggaattt
    11521 catcgtttac attgggagcc gactgaacat atccgtcgag gacaatggct acctgtttcc
    11581 cgatgttatc ggcggtaagg cgtttccatt ttttggcccc ttctgaattc atgttcatcg
    11641 aaacttcaac gcggccattc tggtcataat cctgccgtgc atccacaacc accgatccgt
    11701 caagggctgc agtaccttca cgggtgctgg ctttgagggc cgtcagttca agcacgtcgg
    11761 gcatctgggg cgatggtttg gcagtccatg ccaatacaag gttctgcggg aaaatgttct
    11821 ttaccttttt gagcagcgca ttaacgcggg cggtatcttt tatggcagcg tatcctaccg
    11881 tagccctgtc gctggcatag tactggttgt tttttccctg ataataggcc gggcgcatgt
    11941 aagcaaacaa cgggttctcc ttggcataat tctcgaatga attatccttc tttttgccgg
    12001 tcgtatcgct tttaagtttt tcggcaagcg ttttcttagt ggtatcagct ttggcagcaa
    12061 cggttgaatc ggcggccttt tttgccttta tcgtatctgt ggctgctgca agggtgtcta
    12121 atatgttacc ggaagctaaa cgtttgttgg cctcggtaaa gtaactgctc agtacattga
    12181 actgatatgt ttcccagaat tcgagctggg cagttccctg taaaagtttc ctgacacggt
    12241 caggttcctt gatacccgga agttcaatga ggatgcggtc cgaagtctgg agtttctgaa
    12301 cgttgggctg tgcaacgcca aaacggtcaa tacggttgcg caggatgttg aaggtacggt
    12361 cgaaagcgtc ggtacattcc tggcggataa cttttaaaac ctcttcattt gttgaattgg
    12421 gcttgatctt atcgcgcagt tccatacgtc cgaaaatggc ggacagtttt gcgttgggat
    12481 caatctcggt gaaggactct ccgaataaag tcacaaagtc gctctggctg tttttcatct
    12541 tctcgttagc catgttcatg gcttttacga aagtctcatt ggtgctgttt cccgacatag
    12601 cctttacgac atcggaaacc gatacttcca tcgtaacgtt cataccgccc ttgaggtcga
    12661 gccccaggtt tatctcatgc tcgagacatt ccttgtaagt gtacttcctg atcaggagat
    12721 tgtacacagg ctggttggac accgaatcga gaaagtactt tttgcgaagc tgcaccagcg
    12781 aatcgagaat atcaatctcg cgggctgcgt ttcctttggc taattgtttt gcctcagcgg
    12841 tcactgcttc tgtttgggcg aatcgaacgg cttttttctc cgttatccgg gcaacgattg
    12901 tgaacgaaag ttggaaaagg cacaccaggg cgaatgcaat ggcaaaaaaa cgaattgcgc
    12961 ctttattctg catattgtta ggttttagat agttaggttt attctgattt ctaagcctgc
    13021 aaaggtagaa attgttactt aaattgcaaa tggagaaatt aattcaaagg tcattaataa
    13081 tccccggcgg tttgaaggca gcggcctttc cccgttcctg aaaggcgtta tgtaaggatc
    13141 gacggcgccg gcgcgggaag atatactccg gtttgtctca tggcatttcc cggtgaccgg
    13201 ttatccggga ttcttatttt ctattaatct tacctttgta acgcttcaat atcacgatca
    13261 tgacaaggca aattcttttc gcggctgtct taacggtaat ttccggctgt ctgtcggcgc
    13321 aacaggaaaa tttcagttcc ggcgatccgg taaccgtcgt aacctggaat atgaggtaca
    13381 acaatccgga tgacgggcaa aatgcgtggc caaaccgcaa agctgcattt ctcaggattc
    13441 tgaaagatga aaaaccggat atcttcgggt tacaggaagc cctttatgac caggttcagg
    13501 atgtggaaaa ggcttttccg cgatttaagc gggttggcgt cggacgcgaa gatggcaata
    13561 ccaaaggcga atttgcggtg attttttacg atacggtgaa attcaggagc ctttcctccg
    13621 gaaatttctg gctttcccaa acgcctttcg tgccgggaag catgggctgg gatgcagcct
    13681 gcacccgcat tgtgacctgg acgcaattac aacgcaaaaa cggcggcgct gaactttttg
    13741 tttttaacac ccatttcgat cacataggcc aggttgcccg ccgcaacagc gcctggttat
    13801 tgcttcatgc cgtcgattca ctggccggga agcgcccggc gatcatcacc ggcgatttta
    13861 acgcccagcc gggatcggaa ccggtcagca tccttaccgg taaaaataac cccatacact
    13921 tcatcaactc atccgacatt gccggagttg ctgattcggc ctcctttaca tacgccggtt
    13981 tcaaaccggg ggaaatacaa ggtgaaatta tcgactacat ctttataagg aatatccgga
    14041 aagtgtccga atattccgta aatacctgtc atgatggcga ttattaccca tccgaccatc
    14101 ttccggtgaa ggcaaaagtt ttattttaat ttaacgtttc agcatgcttt cagtaaaata
    14161 ttcctggctt atcggccttt ttattctgat cccttttaca ggaaagtcgc agtcctccct
    14221 gaagatcacc caaccggtga cggctggccc ggatgttttc gatgaaaacg gaaaccggct
    14281 gagcatgcct ttcgcaggcg gactgaatgc ctgccagtta ggcgctgtcg acctcaacgc
    14341 cgatggcgtg gaggacctcg ttgtttttga ccggcagggt tacaggttgt tgacttttat
    14401 caatgaaggc ctggccggtg agatcagtta cgtttatcag cccgtgtatg aaaagtattt
    14461 tcctcctgtc gaacactgga tgcaactggt tgattacaac aacgacggca aagaggacat
    14521 ctttacttac accaccggcg gaatcaaagt ttaccggaac gactcgcagg atgtgccggt
    14581 ctttacgcag gtttccaaac cttacctcgt ttcgttgcag ggaacaaccc tgaccaatat
    14641 tttcgtaacc tatgtcgatt atcccgccat tgttgatgtt gacaacgacg gcgacaagga
    14701 tatccttact ttctgggggt tagggtcctt tgttgaatgg cataaaaact tatccatgga
    14761 gaacttcgga acagccgatt cgctgatctt tgaaaaagtg agcgattgct ggggcaggtt
    14821 tgccgaaggc gctgagaaca acagcattca acttgacacc tgcgtaagtt tcgatgaaaa
    14881 aatgcaggcg tattccggat taaagggcga cgacccgaaa cacacgggtt ccaccttgtt
    14941 ggtacatgat tttaatggcg acggcctgcc cgacctggca ttgggcgacg tggattatcc
    15001 cgccctgatc cggtttacca atggcggtac atccgaagaa gcaaagatgg tttcctttac
    15061 cgattcattt cccggggacg tcaatccggt gcatataacc tctttcccgg catgttcggc
    15121 agtggatgtc aacaacgacg ggcgcaccga tctcatcgcc tcgccattcg agcccagcct
    15181 gatccgttgc gacggcttca acagtaactg gctttacctg gacgagggaa ccggaaccat
    15241 acccggctat aactttattc agaaggattt cctgcaaaag gatatgatcg acctgggatc
    15301 gggagcgttt ccggcgctat tcgattacaa cggcgacggc cttcaggacc ttattgtcgg
    15361 taattacggg tatgttgaca gcagttggct tgatcctgtc tatggactga aatgcgaata
    15421 tgtttcttca ctggcattgt tcgaaaacac gggtacggcc gacctcccgg cttttaaact
    15481 ggtaacgcgc gattatctta acctgagctc cctgcagatg cagtcactga ttcctgccgt
    15541 cggcgatgtg gatggcgatc ataagacgga tatcctgtgc ggaaattcaa aggggaaatt
    15601 tgtctttctg aaaaataaag cctcttcggg gcagcctgcc gattttgaac cggctgatcc
    15661 tgcctttcag caaatcgacg tcggcgattt cagcgctccg caggtggccg acctcgatgg
    15721 cgacggactg aatgaccttg tgagcggcaa caagaaaggg aagctgagct attaccgcaa
    15781 taccggccag gccggcagcc cgtcgtacac gctggttacc gattccctcg gcgggataga
    15841 tgttaccaac accgcggttt cctattacgg atacagcatt ccgctgtttt ataatctgaa
    15901 tgacgggcag aaaatgcttc tcgtcggttc ggagtttggc gaaatctttg tttacagcgg
    15961 catcgaaaac aacctcgacg ggcattttac attcgaaggg ctgctgcagg gccttgaaaa
    16021 agggtggaga agttcgccgg cgttgggaaa catcgacggg gatacgctga cggaattgtt
    16081 tgtcgggaat tattcaggcg gacttacctg gtacaaggga taccatgcgt cgcccgacgg
    16141 ggtgggagag gaacggccgg ccggaaaaat acggttggaa ataattccga atccggcttc
    16201 tgataaggcg accatcatga ttgaagggct gaaggaagat ccgtccggta tccggatatg
    16261 cctgtacgat atgtcgggcg cccggaagta cgcgtcagag cgctgcgggg acaggactga
    16321 aattccgctg aatggttttg ctccgggact ttatcctgtc gtggtaacat tggaaataaa
    16381 gggcgcggtc acccaggcga cagggaaaat tatggtcgtc cgctgattca tttatcaatc
    16441 cggggcagtt gacatagtcg cccgatatag ttgaaaaagg gattttaccg gcccataacc
    16501 tgccggtaaa agtaaactcg ttgccggtac cggctttata aacggaagat caatgtttta
    16561 tgtatgccat gtaaccggtc cttattctat ttgcctcctg tcggtttata gtccggtaat
    16621 tcactcagcc tgtcaggcaa caggtctatg ccttgaaact catttactac aaccgacttg
    16681 atcttatcct tgattttctc atccacgtat gtaatcgaca tctgccagcg gcctccgata
    16741 ctcttacagt tatcttcgac caggatgtcg ggcttgatcg tttcaacgat gtctttgtat
    16801 ctttctcccc ttgaacggta atacaagaaa gatcccggga acccatattt tatcagtaaa
    16861 gccctgatcg attgcgccga agattccctt cttccggaag tcagataaac aatccgggcc
    16921 ccgtcatgat cccagttact gatcttagca acgcagtttt ttatcggaac atacctggca
    16981 tgattgaaat ggtcaatcaa cctccgcggc cccaggatcg ttccttccgt aaaaatcatt
    17041 acggtcagtt ttttataagc agcgtcacct gaattatgta ttgctagtct cttcattgaa
    17101 accggttttt ataggatgtc ttacgccggg ctgtttaaca gatcaacggc cgcccattac
    17161 aaccgtaacc agaacggaca tcctttgcgt tttgcggctt accgaacctg attacgttca
    17221 cacgggaaat gtatcaaaat tacaggcctg ttctgcatta tccgtgctgt aactgcatgg
    17281 accggtgaag gccagcggtg ttatttccag atattggctt tctcaagata ttcattggcc
    17341 cgggactggc aattattggc ttttttcagg tcatcaaggt ttccgtaagc cttgtatttg
    17401 ctgaaatagc tgtcggcttc cgcctgttcc tgttcatata gattgtagta tgtatggtac
    17461 ttgtctttac catactgcaa ctctctgctg acggctttgt ccgtcgcgtt gttgcggtac
    17521 ggatcgcttt ccggctgagc gctgccggcc ggggaattac cgtaattcgc ggcgttgtta
    17581 taggcgctgt tgagcggatt cgaaaagctg gctgccgcgt cgccaatggc tttgctcagc
    17641 atgatgatct tcgtgttctt aatggcttca atagcttccc ggtaatcagg atcaaccgaa
    17701 agggatttgt taaagaaagt gagggcgtcg ctatacagtt cgcaggcaaa aaagcaatac
    17761 cctatacagc aataaggggc agggtctgta ttgttgagcg aagcagcttt tgcataattg
    17821 ccgacagcat tcacataatc cttcgctgaa aagcatttga agccttcgtt acaaagggtc
    17881 atggcttcgg caacccgctt gttgtgatcg tttacaacct ccatgttttc cagtgcaatc
    17941 cgattatcct tgtcaagttc aagagctttc ctgaaatcag ccccggcttt cacgtaatct
    18001 tcaggttgcc gggtaaggta gcaatagccc ctgttaatgt aagcattcgt gtttttctca
    18061 tcgtaagcaa gcaaagaagt aaaccggtcg atggctgacg cgtaatctcc cttgtccatc
    18121 aggctcacgg catcccggta agccagttca cagtatagcc agcggcaaac ataaatgccg
    18181 ccatccaggg cttttccggc ccaattcaac gcttcagcat cattcttctc cattccttgt
    18241 ccgtcataga gcatgagggc catgtaatat gccgattcgg catctccttt ttgggccgtt
    18301 ttcaggtacc actttgcagc ttcctggtaa tcctttccgg tccctttccc gaaatagaag
    18361 caatatccca gttgcttaca ggcgttttca tcaccctgtc cggcggcctg ttgatagtat
    18421 ctgacagccg tggccggatc ctgttttaca ccgatcccgt tgaaataaca atcgcccaga
    18481 aaactttgtg ccgaaacatt tccattgccg gcagccagcg tataccattt gatggcttcc
    18541 tcgtggttct tgtccggaat caggcctttg gaatacatat cgcctaacgc tgcctgcgat
    18601 aaaacatagc cttgggctgc cgatttttcg aaccatgccc gggccgtttc ctgatctttt
    18661 aacaaacctt caccattcag ataactcatg gctaaccggt actgatacga agggtcgcct
    18721 tgccgggcca tctcggtcac tgccctgata tcaacatcat aaacaatgtt gccgtccagt
    18781 tttgaacagt tgctgaacac gtccggcgcg aaaatcagat ctttattccg gcatatcacc
    18841 gatttcaggt tataacagta gttaaaagcc tgcgagccga ccttgcttac cttttcgggg
    18901 atggtcacgg atgtcaggtt actgcagttg tcaaaagcat gatccccgat ttcggtgaca
    18961 ctttccggaa ttgccaaaga atcaaacctg caatactcaa aagcattgtc tccgatacgg
    19021 ttcagatgat cagggagcac aattatcctg agatttgaac agaaagaaaa agcgcgctgc
    19081 tcaaccgata ccaccgatga aggaagagtg taccccgtct cattccggcc ttgcgggaaa
    19141 cggtacatcc gggtcatgtc cttgctgaat aaggccccat cggctgattt ataatactca
    19201 ttttccgaag ataccgtgat ctgtttcaaa tccgacagat gataactgaa aacgccggcc
    19261 tcaatgttgc tgacactgcc ggggatcgtg atggaggtga tcctgctgca gccggaaaac
    19321 gcacctgcct caatcgtggt gaccgggtac tgtttcctct tgtatttgac ttccggcgcc
    19381 aggaccacat ctcccgtcag cgttcccttg taagtgaagg ctgttgagga aggatctttt
    19441 acatagttct gcccgacgct tgccgtgccg ctcgctatat cgacgttata cgccactccg
    19501 ccggatttaa cggttatttt ttgagagaaa acggcaggaa taccaagaaa gggcagcaac
    19561 aggagaaaca attttttatt catggggcaa cggggcaatg gttttacggg gcgtgggcgc
    19621 ttttgtctgg cagggttatc aagggttgaa ttaaccgccc gctcacaata tgacaaccgg
    19681 aaccaaccgg cagccggaat aaattgggtc atacaaaact aacgcaggcg gtttacggct
    19741 gcaaaagtat aaaagaacaa ggtgcaggtt tccttgaaaa acattgca
//
LOCUS       DAOPYV010000058        19668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_2875_length_19668_cov_10.954571, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000058 DAOPYV010000000
VERSION     DAOPYV010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19668
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2875_length_19668_cov_10.954571"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PKI35_08410"
     CDS             <1..411
                     /locus_tag="PKI35_08410"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNX44441.1"
                     /translation="LILCTGNSCRSQMAHGFLQSFDKSLTVCSAGTNASGKINEKAIE
                     VMKETGIDISRHTSDSVELYLNDEWDYVITVCEDANEACPVFPGKVKHRLHMGFDDPS
                     QVTGTDGHILSEFRRVRDEIREGFRKFYVENIRQ"
     gene            464..1300
                     /locus_tag="PKI35_08415"
     CDS             464..1300
                     /locus_tag="PKI35_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNX44442.1"
                     /translation="MNNEELKNVVKKKYGEIASQNRQTNVTSCCGSTGCCSDVDYTIF
                     SERYDKLEGYNPDADLGLGCGIPTEFAQIKAGDTVIDLGSGAGNDCFVARALAGEKGK
                     VIGIDFTPAMIEKARENAEKLGYNNVEFRFGDIEKLPVTANKADVIISNCVLNLVPDK
                     KKAFNEIFRVLKPGGHMSVSDVVLKGNLPEKIIGAGEMYAGCVSGAIQLDEYLAVIEN
                     AGLKSIIVQKEKQVVIPDEIMLEYLSPAELIEFKGSGAGIFSITVYAEKSVSQCCSGC
                     GC"
     assembly_gap    1312..1411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1726..2823
                     /gene="arsB"
                     /locus_tag="PKI35_08420"
     CDS             1726..2823
                     /gene="arsB"
                     /locus_tag="PKI35_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNX44443.1"
                     /translation="MPSKPRLKFLDRFLTLWIFLAMFIGVFSGYLFPSIAQFWDSMKS
                     SPESTTNIPIAIGLILMMYPPLAKVKYEELGDVFRNRKVLALSLIQNWIIGPVLMFAL
                     AVILFKLFPGENNSNLPYMYGIIMIGLARCIAMVIVWNELAKGDTQYAAGLVAFNSIF
                     QVLFFSVYAYFFIAVLPGWFGIPTNDAVENITIGQIAESVFIYLGIPFIAGFLTRFIL
                     IRIKSKEWYHNKFIPKISPLTLIALLFTIIVMFSLKGEYIVKIPLDVVMIAIPLLVYF
                     LIMFMLSFFMSKRVGANYGQAVTLSFTAASNNFELAIAVAIAIFGMNSGEAFAAVIGP
                     LVEVPVMIGLVNIAFLFGKKYFSTQKELQAE"
     gene            2858..3175
                     /locus_tag="PKI35_08425"
     CDS             2858..3175
                     /locus_tag="PKI35_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44444.1"
                     /translation="MKVLAFILSLYVVALTLTPCVDRLPDKHAVNNNNMTQPCSDRHH
                     QEGADHCSPFCTCNCCATPVIIQDFSVHFECYPFMRRQEFANVSHQVSSPVIAIWEPP
                     KIG"
     gene            3267..7658
                     /locus_tag="PKI35_08430"
     CDS             3267..7658
                     /locus_tag="PKI35_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNX44445.1"
                     /translation="MIERIIHFSIKNKFIVGLFVIALIGWGTYSLTRLPVDAIPDITN
                     NQVQIISLAPSLAVQEVESFITAPVEVAVANIPDIIELRSISRLGLSVVTVVFKDGVD
                     IYWARQQLTERLREAEEEIPDGFAKIELAPISTGLGEIYQYRLAVDKGYETKYTPMEL
                     RTLQDWVVRREMLGTPGVADINSYGGFVKQYEVAVNPERLRGMGLTLTDIFDALERNN
                     ENTGSAYIDKKPNAYFIRSIGLVGSLADIEKIVVKTNASGIPVLIRDVATVQYGSATR
                     YGAFVVDTAEAVGGVVMMLKGANAHQVIDNVETRIESIQKSLPEGVTIEPYLNRSELV
                     SRAIGTVAKNLIEGALIVIFILVLLLGNFRAGLIVASVIPLSMLFAISMMNIFRVSGN
                     LMSLGAIDFGLIVDGAVIIVESAVHRVFMSKSHHQGLARLSQQQMDENVLESAKRMMS
                     SATFGQIIILIVYLPIMALVGIEGKMFRPMAQVVAFALTGAAILSLTYVPMAAALFLS
                     RNTSHRRNFSDRLMDWFHKMFTPVINFALRRKLLVSASAILLFIFSLFLFTRLGGEFI
                     PQLEEGDLAAGVMTLQGGSLSHTVDMVEKANKILLKNFPEIKHTVCKIGAGEIPTDPT
                     PMETGDYIITLKPKSEWTSAETLEELVAKMEDKLAVLAGVKFEFQQPIQMRFNELMTG
                     SKQDVAIKLFGDDLNTLAAKASEIEKVIDRVEGVEDINVEKVTGLAQVQVEFDRDRLA
                     RYGVSIEEANRILRSAFAGSQAGVVFDEEKRFGMVVRLDKDYRQNLDDVKTLAVALPD
                     GGQIPLEQIASIEIKSGPAQVSRENARRRITIGFNVRNRDVQGVIAEVTNKIDAKVNL
                     PTGYYIDYGGQFQNLAAAKRRLSIAVPVALLLIFVILYLTFRSLRETLLIFSAVPMAA
                     IGGVFALWIRGMNFSISAGVGFIALFGVAVLNGIVLIAEFNRLEKEGITDIHQRVLKG
                     LHSRLRPVLMTAAVASFGFLPMALSTSAGAEVQKPLATVVIGGLITATILTMVILPVF
                     YVFFTETRIKPLFTKKTVTPLLVAAFLISANAFFNPAQAQKTGRITLEDAVKMALDNN
                     LALRSSAYSVEEQKMLKGASWEIPKTSVEFGYGQFNSYSKDNSITVSQSVLFPTVYTN
                     RSKLAAATIRDSEWKLKQSRLETATEVKRVYWQLVYLMEKKKLLVCQDSLFSGFCKAA
                     ELRAQTGETNRLEMITARSQNLEIRDRLQQTESDMGIYARKLRTLLNTQDLYLPSDSL
                     FKPVMPVIAGDSAFLSQNPFLGYYLNQAEVENLNRKLEISLALPEFSVGFSSQTMQGV
                     QEINSIPRMFGTDERFNGFQAGIALPLWYKPYSAKIRAAGIRQDEARINAEAYARSLV
                     SEYHNLLDEFDQHKKSLEFYEQQALPEADLIIGQSGKSYTAGAMDYLDYVMSLNRALT
                     IRQNYLEALSSYIQTYINIGYITGKIM"
     gene            7670..8851
                     /locus_tag="PKI35_08435"
     CDS             7670..8851
                     /locus_tag="PKI35_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX44446.1"
                     /translation="MKINKIRSLIYFAALLAVLPSCNKEKKSSGGEDAEVIPDDIVEM
                     REDQIKLAGIEFGAVEMRSLSGTINAGGTILAAPQNMATVSAPLGGFVRSSFLLPGNA
                     VAKGQTLAIIENQDFIDIQQNYLDACNKLEFAEAEYKRHTELYKDDVYSQKSLQQVTS
                     EYRSMKTQVKALEQKLMLIGINPAKLHEDNITRSVAIVSPITGFLKTVNVNVGKYVTP
                     ADVMFEIVNNDKLFLELTLFEKDVDRVSAGNNIRFFLNNAAEQHNATVYQVGKAVNAD
                     KSFRVYASVNDKCKNMISGMYVNAVIEASSSRVESLPSEAVVSFDDKDYIFVFDKNKQ
                     ENGKPFTEYRMVEVHKGVSEGGYTEIIFPEGFDTRAAKVVVKGAYNLLSAKKNAGEMA
                     C"
     gene            complement(8973..9944)
                     /locus_tag="PKI35_08440"
     CDS             complement(8973..9944)
                     /locus_tag="PKI35_08440"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF000824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HNX44447.1"
                     /translation="MKTNHVSEQRRVVVVGAGAVGATYCYALAQSGLADEIVIIDKNE
                     DLMHGQVLDLVHGQPFFPTVAIREGTPEDYTRAQLVVITAGIAQRPDETRLQLLRKNA
                     QVVGDIAADVAAHHCEGVMLIVSNPVDVLTYIAVKRSGWDRSRVIGSGTVLDSTRFRH
                     LLSMHCGVDVHNVHAYMLGEHGDSEFAAWSMTNIAGISIHDYCPVCEKCSDWKNQQKL
                     IEQQVRDSAYHIINYKGATHFAVGLALVRITGAILRNQSSVLTVSILVNGEFGIQDVC
                     LSVPCIVTARGVSAIIRSPLSEEEFSQLRHSASVLRNAIGSLEEEPQ"
     gene            complement(10218..10511)
                     /locus_tag="PKI35_08445"
     CDS             complement(10218..10511)
                     /locus_tag="PKI35_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44448.1"
                     /translation="MKAVFIAYNQSLTEGVQNILDDLAIRGYTQWTDIKGQGTEKGDP
                     HHGTHTWPSLNNVHLTIVPDDVVEPLLEKLDFLNKEVEEQGLKAYVWNIEASV"
     gene            complement(10521..13652)
                     /locus_tag="PKI35_08450"
     CDS             complement(10521..13652)
                     /locus_tag="PKI35_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX44449.1"
                     /translation="MSLYSTAVKKPVTTIMICIGVIILGIYSYLQLPLDFFPKMDPPY
                     ISVITYYPGANAADVEQNITRKLEDGFGSLSNLKNMTSTSKDNISVVMLEYEWGANLD
                     EATNEVRDAVGMTQNRLPDEVETPVIFKISTSMIPVIMFSVTCDESYEGIQDILDDKL
                     VQPLNRIDGVGSIYQIGAPVRAVMVDIDPRKLDAFNLTVEQIGGILAANNLNLPSGNL
                     KMGKADLALRLQGEFENSDVVKNIIVSNYNGKTVYLKDIASVRDSLKDINSYERANGQ
                     KNVRIMIQKQSDANTVAVANQVKKKLEEIKKTLPPDVKVQVLMDTSVNTIDSINNLSE
                     TLIYALIFVVLVVFFFLGRWRATFIVSLSIPISLIAGFIYMFLSGGTLNIITLSSLSI
                     AIGLVVDDSIVVLENITKKLEKGGFTREAAIYGTSEVSLAVIASTLTIIAVFLPLTML
                     GGMTGIIFKPLGWVVAITVAVSVIVSMTLTPMLSSKILSAKLPSTDTPAGKLYSFSQG
                     LLRRLDNFYEKILFWAVNHRWTIIIIAGVIFISSMVLVKFIGTEFMPASDNDRISAQV
                     KLAQGSRIEETAKTAHYLDSVFMKKYPEIELTSTSAGAGDGSNSMSAVFSETGNYIIN
                     YTFKMTPHQKREKDIFQIAEEMRKDIEVLPEVEKFYVDPGSSRSSGGMGSGGGSVLEI
                     KVYGNDFDKTNVVAENISNALKGIKGAEDIKVSRDKDKTELQLVLDHEKMTSFGLNTA
                     TVANAVRNRINGLTATKYKEAGNEYDVIVRYDDKYRQSTEDIENISIATPQGKIIKLS
                     EIATLKRFYSPPSIERENKVRIVTVSAAISGTDIGTVQAGLDREIAKMNIPDDVSLEY
                     GGSIEDMQESFSNLFLLLLLSIILVYIVMASQFESLREPFIIMFSILFALTGVFLSLW
                     IFHSTINVISMIGLVMLIGIVVKNGIILVDYTNLLASRGYQLKEAVIAAGRSRLRPVL
                     MTSLTMILAMIPMIVSTGSGSEMWKPMAIAIFGGLTFSTLVTLILVPTIYTIFGIGKM
                     KRLKRAEITLANQ"
     gene            complement(13702..14757)
                     /locus_tag="PKI35_08455"
     CDS             complement(13702..14757)
                     /locus_tag="PKI35_08455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX44450.1"
                     /translation="MNTNKFLLLSIIAGIVTLQTACSSKKDNAAANAKTAVQQTVPVK
                     TEVVGKESVARTIEYTASLLPYQEVHLAPASPGRIDKINVNISDRVRAGQALVMMDRT
                     QLEQARINLVKLESDFRRFDTLKKTGSIADQQYDQIKTAYDVAKTSYAFLQDNTQLKA
                     PFDGIVSGKYFENGEIYSGSPAAAVGKPAIVSIVQISQLKALVSISSMYFPLVSQGMK
                     AVIESELYPGIKFTGDISRIYPTMDNATKTFTAEVRIQNGQLKLRPGMFAKIMLDLGE
                     GEAILVPNISIIKQTGTNNMYVFVNKNNVAVKTPVITGRMFDDKIEILSGLNSGDEVV
                     IVGQNKLENQSPISVIK"
     gene            complement(14768..16066)
                     /locus_tag="PKI35_08460"
     CDS             complement(14768..16066)
                     /locus_tag="PKI35_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX44451.1"
                     /translation="MKRISRAALYCVFCLGTMSLQAQEPLVLSLDSAITYATDHNRTL
                     IGSRLAVDKSTEKIRETVAQGLPQVSASMDYTNYLGAEASLEMAPGQSISIEFNPTSS
                     FKASVTQLVFNGSYYVGVQLSKLAKTITEQSYRKDELNVREQTIQAYYTVLVTEKLLN
                     IIKANKENALTMFEKTTNLANAGIVEQTDPKKLSIMVSTVDNAIKSTERQLELGYNLL
                     RLQLGLESGQEIQLSSSLAELAEKCTLYGASDKQFDIGNNMDFKLISMQGDIAKKQIE
                     LKQSSYLPTLAAYYSRTQKIQTAKFDMTPNNVLGLSLNVPIFSSGQRKAQVSQARIDL
                     DINKNTQALLTQQLTLQEKQLRFNYNNLLEQYRNQQRNVEIAKEVLEKMKLKYQQGVI
                     SSLELTSANNDYLTAESNHAGTLLQLLNAELALRKINNNL"
     gene            complement(16186..16797)
                     /locus_tag="PKI35_08465"
     CDS             complement(16186..16797)
                     /locus_tag="PKI35_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX44452.1"
                     /translation="MTVREKIVSGANQLFLKYGIRAVTMDMIAQELGLSKRTIYENFS
                     DKDALIRDCIDMAMKEKERHFDESIQNSENAIDALISMMKFTIQAINRINPLFFTDIQ
                     KYFAEINVSKIKSGENCMATEIVSILNKGVQEGLFRKELNMDLVARIFSAQSGVMKNQ
                     ELYSTERYSKEEILQTLMLNFIRGIATDKGLTIVNQYTGNASV"
     gene            complement(17088..17612)
                     /locus_tag="PKI35_08470"
     CDS             complement(17088..17612)
                     /locus_tag="PKI35_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HNX44453.1"
                     /translation="MTEARIELIRGDITVLTADAIVNAANSSLLGGGGVDGAIHRAAG
                     PHLLEECRTLGGCPAGEARITNGYRLAAKYVIHTVGPVYHGGAHDEPVKLAACYRNSL
                     KLAVENGCKTVAFPNISTGVYSYPKNEAAGIAVAEVKHFLTENTSLEKVIFCCFDQEN
                     YNIYREILSGEIHA"
     gene            complement(17649..>18422)
                     /locus_tag="PKI35_08475"
     CDS             complement(17649..>18422)
                     /locus_tag="PKI35_08475"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNX44454.1"
                     /translation="ELSRRTGQFQKDEINKVGNADVEEKGLHNFVTYVDKTCEKQIVN
                     ELQQLLPEAGFIAEEGTSDRKGERFNWVIDPLDGTTNYIHRIPLYSVSIALMDGAETV
                     LGVVYEPNLDECFYTWKGSPAFLNGKAIAVSPLSQMTKALLATGFPYYDYGRMKSYMD
                     LFRYFAENTSGLRRLGTAAIDLAWTACGRCEGFYEYALHPWDVAAGALIVQNAGGKVT
                     DFRGGKDYIFGGEMLASNNILHDELLRSVKEYFPEKPAI"
     assembly_gap    18423..18522
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18523..19277)
                     /locus_tag="PKI35_08480"
     CDS             complement(<18523..19277)
                     /locus_tag="PKI35_08480"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNX44455.1"
                     /translation="MFPRFSDMLNYLFGTHWNLPVQTYGFFLALAFLVGATVLYYELK
                     RKEKEGLLLPKQRKVLKSKPPSTSEILLGAIITFLLAWKVGGIVTDYPVFAANPQRYL
                     TSASGNLLLGILAAAVIIVYEIIRQKKLKNTTAEYIDEIIHPADYTWNILIIAVVAAL
                     IGSKLFDIFDNFGDFLRNPVGSLLSFSGLTFYGGLIVTVITLLWYLKKIHLDWHYVID
                     AAAPAIMIGYAVGRLGCQFSGDGCWGIVNTMAKP"
     gene            complement(19375..>19668)
                     /locus_tag="PKI35_08485"
     CDS             complement(19375..>19668)
                     /locus_tag="PKI35_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNX44456.1"
                     /translation="AVELEGHVDEKEKHRRSMVLHELSEAKKLAFYQAHTGKTFSVLW
                     ESDNVNGMMHGWTENYIHVRTAYTPGLVNTITPVRLLSVAREKELICDVLVEP"
BASE COUNT         4910 a         4500 c         4794 g         5264 t
ORIGIN      
        1 cttatcctgt gcaccggaaa cagttgccgc agccagatgg cccacggatt tctgcagtcg
       61 ttcgacaaat cgcttacggt ttgttcggca ggtaccaacg catcgggaaa aatcaacgaa
      121 aaagctatcg aggtaatgaa agaaacaggc atcgacatca gccgtcacac ttccgattcc
      181 gtcgaattat acctgaatga cgaatgggac tatgtgatca ccgtttgcga agacgcaaat
      241 gaggcttgtc ccgttttccc gggaaaagta aaacatcgcc tgcatatggg tttcgatgat
      301 ccttcgcagg tgaccggcac ggatgggcat atcctgagcg agtttcgcag ggtgagggac
      361 gaaatcagag aaggattccg gaagttttac gttgaaaaca tccggcaatg ataaactcaa
      421 ccattttcaa attcacacac tttcaacttt ttagattcat accatgaaca acgaagaatt
      481 aaaaaatgtc gttaaaaaga aatacggtga gatagcaagc caaaacaggc agacaaatgt
      541 aacttcctgt tgcggttcaa caggttgctg cagcgatgtg gactacacga ttttcagcga
      601 gcgctacgat aagctcgaag gatataatcc tgatgccgat cttggcttgg gctgcggtat
      661 tccgactgaa tttgcacaga tcaaggcagg cgatacggtg attgacctgg gatcgggcgc
      721 gggcaacgac tgttttgttg cgagagccct ggcgggagaa aaaggaaagg tgatcggcat
      781 tgatttcacc ccggctatga ttgaaaaagc ccgtgaaaat gcagaaaaac ttggctacaa
      841 taatgtggaa ttccgctttg gagatattga gaaacttccg gtaactgcca acaaagccga
      901 tgttatcatc agcaattgcg tgctaaacct ggttcccgac aaaaaaaagg cattcaacga
      961 aatttttcgg gtgttgaaac ccgggggaca tatgtcggtt tccgatgtgg ttctcaaagg
     1021 aaatctgccg gaaaaaatta ttggtgccgg ggagatgtat gcagggtgtg tttccggggc
     1081 gattcaactg gatgaatatc tggctgttat tgaaaatgcg ggcttgaaaa gtattattgt
     1141 tcagaaagaa aaacaagttg taattcccga tgagattatg ctggaatacc tttcacctgc
     1201 tgaactcatt gagttcaaag gttctggagc aggtattttt agtattacag tttatgccga
     1261 aaaatcggta tctcaatgtt gcagcggatg tggttgctaa aggtgatttg annnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaaatgaaa attaagatct tcaaagccat
     1441 gaaaataata acctgatagc gggtttaccg acagccacaa attaatttga aaatttgaaa
     1501 atgtggaaat ttgaaaatga aaattaagat cttcaaagcc atgaaaataa taacctgata
     1561 gcgggtttac cgacagccac aaattaattt gaaaatttga aaatgtggaa atttgaaaat
     1621 gaaaattaag atcttcaaag ccatgaaaat aataacctga tagcgggttt accgacagcc
     1681 ccattctcaa attctcaaat tgtcacattt tcaaattcat aagatatgcc atcaaaacca
     1741 cgtttaaaat tcctggatag gttcctgaca ttatggattt tcctggctat gtttataggg
     1801 gtgttttcag gatacctgtt tccctcaatt gcccaattct gggattcgat gaaatcatcg
     1861 cccgaaagca caaccaacat acctattgca atagggctga tactcatgat gtatccgcca
     1921 ttggccaaag ttaaatacga agagctgggc gatgttttca ggaaccggaa ggtgcttgcc
     1981 ttatccctca tacagaactg gatcatcggc cctgtgctga tgtttgcgct ggcggttatc
     2041 ctgttcaaac tgttcccggg cgagaataat tcgaacctgc catacatgta tggcatcatc
     2101 atgatcgggc tggcccgttg catagccatg gttatcgttt ggaatgaact ggcaaaaggc
     2161 gacacgcagt atgccgccgg gctggtggct tttaactcca tcttccaggt gctctttttc
     2221 tcggtatatg cttatttttt cattgcagtt cttcccggtt ggttcggtat tcctacaaat
     2281 gatgctgtcg aaaatatcac catcggccaa atagccgaga gtgtattcat ttacctgggc
     2341 attccgttta ttgccggttt ccttacccgg tttatcctga tccgcattaa gagcaaagag
     2401 tggtatcaca ataaatttat tccgaagatc agcccgctga ccctgatagc ccttttgttt
     2461 actattatcg tgatgttctc gctcaaaggg gagtatatcg tgaagattcc ccttgatgtg
     2521 gttatgattg ccatcccgtt gctggtttat ttcctgatca tgttcatgct ttcctttttc
     2581 atgagtaaaa gggtaggtgc caattacggg caggcggtta cgctttcgtt cacggcggcc
     2641 agcaacaact ttgaactcgc tatcgccgtg gcaatcgcca tcttcggcat gaactccggt
     2701 gaagcttttg cagccgttat cgggccgctg gtagaggtcc cagtgatgat cggattggtg
     2761 aatattgctt tcctgtttgg gaagaagtat ttttcaacgc agaaagaatt gcaggcagaa
     2821 taactttttc ctgtaatttt acagccttgt aattctgatg aaagtcctgg cattcatatt
     2881 atccctgtat gtcgtcgcac tgacactaac cccgtgtgtt gaccggcttc ccgacaagca
     2941 tgccgttaat aataataata tgacgcaacc ctgcagcgac cggcatcacc aggaaggcgc
     3001 cgaccattgc tcccctttct gcacctgcaa ctgctgtgct acccctgtta ttatccagga
     3061 cttctccgta cattttgaat gttacccttt catgagaaga caggaatttg caaacgtttc
     3121 tcatcaggta tcatctcctg ttattgctat ctgggaaccg cctaagatag gttaatcttt
     3181 ccgtgataat ttatttcaat ctgcttccgg cattccataa tcgggaatcc ggcaggttgt
     3241 attattaacg tgttaaggtt ttacctatga tagaacggat catccacttt tcaattaaaa
     3301 ataaattcat tgtcgggctg tttgttattg cattaatcgg atggggaacc tattccctga
     3361 cccgtttacc cgtcgatgcc atacccgaca tcaccaataa ccaggtgcag ataatttcgc
     3421 tggcgccttc gcttgccgtt caggaagttg aaagttttat tacggcgcct gttgaagtag
     3481 ccgttgccaa cattcccgac atcattgaac tccggtcgat ttcaaggctg ggactatcgg
     3541 ttgttaccgt ggtttttaaa gacggcgtcg atatatactg ggcgcggcaa caattgaccg
     3601 aaaggctcag ggaggcagaa gaggaaatcc ctgacggatt cgccaaaata gaactcgccc
     3661 ctatatccac tggtctgggc gaaatttacc agtaccgcct ggcggtggat aaagggtatg
     3721 aaacgaagta taccccgatg gaactccgga cactccagga ttgggtcgtg cgccgtgaaa
     3781 tgctgggaac gccgggcgtt gcggatataa acagttacgg aggcttcgta aagcaatatg
     3841 aggttgctgt aaatcctgaa aggctcaggg gaatggggct gacgctgacc gatatttttg
     3901 acgccctcga gcggaacaat gaaaataccg gcagcgctta tattgataag aagcctaatg
     3961 cctatttcat ccgaagcatc gggctggtag gttcattggc agacattgaa aagattgtcg
     4021 tgaaaacaaa cgcttccggt atccctgtat tgatcaggga tgtggcaacc gttcaatacg
     4081 ggagcgccac ccgttacggc gcatttgtgg ttgataccgc cgaagccgta ggcggggttg
     4141 tcatgatgct gaaaggtgca aatgcgcatc aggtgataga taacgttgaa acgcgcatcg
     4201 agtccattca aaagtcatta cctgaaggtg ttactatcga gccatacctg aaccggtccg
     4261 aactggtcag cagggccatc ggaacagttg ctaagaatct gatcgaaggt gcgttgatcg
     4321 tgatcttcat cctggttttg ttgctcggga atttccgggc gggtttgatc gttgcttccg
     4381 tgatcccgct ttccatgctg tttgccatct ccatgatgaa catcttcagg gtttcgggca
     4441 acctgatgag cctgggagct attgatttcg gactgatcgt ggatggcgcc gtcatcattg
     4501 ttgaaagcgc ggtacaccgg gtgtttatga gcaagagcca tcatcagggt ctggccaggc
     4561 tgtcgcagca acaaatggat gaaaacgtcc tggaatcggc aaaacgtatg atgagttcgg
     4621 caactttcgg ccagatcatc atccttatcg tttacctgcc cattatggcc ctggtcggca
     4681 tcgaaggaaa aatgttcagg cctatggcgc aggtagttgc ttttgccctg accggcgcgg
     4741 cgattctttc acttacctat gttcccatgg ctgcggccct gttcttaagc aggaatacca
     4801 gccacaggcg caacttctcc gacaggctga tggactggtt tcataaaatg ttcaccccgg
     4861 tgataaactt cgcgctcagg cggaaattgc tggtttcggc atcagccatc ctgctcttca
     4921 tattcagcct gttcctgttt acccggttgg gaggggagtt tattcctcaa ctggaagaag
     4981 gcgacctggc ggcaggcgtg atgacgttgc agggtggttc cttatcgcat acggttgaca
     5041 tggttgaaaa agccaataaa atattgctca agaattttcc tgagatcaaa catactgtgt
     5101 gtaagatagg ggcgggggaa atacccacgg atcctacacc aatggaaacc ggggattaca
     5161 tcattaccct caaaccgaaa agtgagtgga cttcggcaga aacgcttgaa gaacttgtcg
     5221 ccaaaatgga agataagctg gcagtactgg cgggcgtaaa attcgaattc cagcagccta
     5281 tccagatgag gtttaatgaa cttatgacag ggtcgaagca ggatgtggcc atcaagctct
     5341 tcggagatga cctgaatacc cttgctgcaa aagcttctga aattgaaaag gtaattgacc
     5401 gggtggaagg cgttgaagac ataaacgttg aaaaggttac cggcctggcc caggtgcagg
     5461 ttgaatttga ccgcgaccgt ctggcccggt atggcgtttc cattgaagaa gcaaaccgca
     5521 tattgcgttc tgcttttgcc ggaagccagg caggggtggt gtttgacgag gaaaagagat
     5581 tcggaatggt ggtccggctc gataaagact atcgccaaaa ccttgacgat gtcaaaacac
     5641 tcgcggtcgc cttgcccgat ggaggacaaa ttcccctaga acaaattgcc agcatcgaaa
     5701 ttaaatcagg accggcccag gtatcgcgcg aaaatgcccg gcgccgcata acgatcggct
     5761 ttaatgtccg gaaccgggat gtccagggtg tgatagccga ggtgaccaac aaaatagatg
     5821 caaaggtcaa tctcccaacc ggatattata ttgattacgg cgggcagttt cagaacctgg
     5881 ccgcagccaa gaggcgtttg tcgatcgcag tacctgtcgc gctgctgctg atttttgtaa
     5941 tcctctacct cactttccgt tcgctccggg aaaccctgct tatcttctcg gcagtgccta
     6001 tggctgctat cggcggagtt ttcgccctct ggattcgcgg gatgaacttc tccatttcgg
     6061 caggcgtggg tttcatcgcc ttattcgggg ttgccgtcct gaatggaatt gtgctgatag
     6121 ctgaattcaa ccgtctggaa aaagaaggta ttaccgacat ccatcagcgt gttttaaaag
     6181 gcctgcattc ccgcctgcgt cccgtattga tgacggccgc ggtagcttcg ttcggcttcc
     6241 tgccgatggc cttatccacc tcggccggcg ccgaagtcca aaaacccttg gcaaccgtcg
     6301 ttatcggagg gcttatcacg gccacaatcc ttaccatggt tattctgccg gtgttttatg
     6361 ttttcttcac cgaaacccgg ataaagccat tattcacgaa aaagaccgtt acccccttgc
     6421 tggttgcagc gttcctgatt tcggcaaatg cgttttttaa cccggcgcag gctcagaaga
     6481 caggaaggat taccctggag gatgctgtta aaatggccct tgataataac ctggcgctgc
     6541 gttcttcggc ttattcggta gaggagcaga aaatgctgaa aggcgcttcc tgggagatac
     6601 ccaagacttc cgtagaattc ggatacgggc agttcaattc ctattcaaaa gataacagta
     6661 ttacggtttc ccagtctgtt ttattcccga cggtctacac taaccgtagc aaactggcgg
     6721 cggcaactat cagggacagt gaatggaaac tgaagcaatc caggcttgaa acagctaccg
     6781 aggtaaaaag ggtttactgg cagcttgtct atcttatgga gaagaaaaaa ttgttagtct
     6841 gccaggacag cctcttttcc ggtttctgca aggctgctga actccgtgcg caaacgggcg
     6901 aaacaaaccg cctcgaaatg atcacggcgc ggtcacagaa cctcgaaatc agggaccggc
     6961 ttcagcaaac agaatcggat atgggtatat atgcccgaaa acttcgtacc ctgctgaata
     7021 cgcaggacct ttatcttcct tccgattcgt tattcaagcc cgtgatgccg gtgatcgccg
     7081 gcgattcggc tttcctgtcc caaaatcctt tcctgggtta ttacctgaac caggcagaag
     7141 ttgaaaatct gaacaggaaa ctggaaataa gccttgcttt gcctgagttt tcggttggtt
     7201 tttccagcca gaccatgcag ggagtacagg aaataaattc tattccccgt atgtttggca
     7261 ctgacgaaag attcaatggc tttcaggcag gaattgccct gcccctctgg tataagcctt
     7321 attcggcaaa gatcagggca gccggcatca ggcaggacga agcgcgcatc aacgcagaag
     7381 catacgccag gtcgctggtg tcggaatatc acaacttact ggacgaattt gaccagcaca
     7441 aaaagagcct tgaattctat gaacaacagg cgcttcctga agctgacctg atcatcggac
     7501 aatcgggtaa aagttacacg gccggcgcta tggattacct cgattatgtc atgagcctca
     7561 acagggcgct aaccattcgc cagaattacc tggaagccct cagcagctat atccagacct
     7621 atataaacat agggtacatc acgggtaaaa taatgtaaaa caatttgtta tgaaaattaa
     7681 taagataaga tccctcattt actttgccgc cctgcttgcg gttttgccgt cgtgcaacaa
     7741 ggaaaaaaaa tcatccggtg gggaggatgc tgaagtaatt cccgacgata tcgtggaaat
     7801 gcgtgaagac cagattaaac tggcaggcat agaattcggc gccgtggaaa tgcgctctct
     7861 cagcggcacc ataaatgccg ggggaaccat cctggcggct ccccaaaaca tggctaccgt
     7921 atcggccccg ttgggcggat ttgtcagaag ctcattcctt ttacctggaa atgcagttgc
     7981 caaaggacaa accctggcga tcatcgaaaa ccaggacttc atcgatatcc agcaaaacta
     8041 tctggatgcc tgtaataaac ttgagtttgc tgaagccgag tataaaaggc atacggaact
     8101 gtataaggac gacgtatatt cacaaaaaag cctgcaacag gttacttcgg aatacagaag
     8161 catgaaaacg caggtaaaag cccttgaaca gaaactgatg ctgataggta tcaacccggc
     8221 aaaacttcat gaagacaaca tcacccgttc cgttgcaatt gtatctccga ttacaggatt
     8281 tctgaagacg gtaaatgtca acgtcggtaa atatgttacg cctgctgatg ttatgttcga
     8341 aatcgtaaac aatgataagc tctttcttga acttaccctc ttcgaaaaag atgtcgacag
     8401 ggtttcggca ggtaacaaca tcaggttttt tctgaataat gcagccgaac agcacaatgc
     8461 aacggtgtac caggttggta aggccgttaa tgccgacaaa tcattcaggg tttatgcttc
     8521 ggtaaacgat aagtgtaaga atatgatttc aggcatgtat gtaaatgccg tcattgaagc
     8581 ctcttcttcc agggttgaat cgttgccgtc ggaagcggtt gttagttttg atgataagga
     8641 ctacatcttt gtttttgata aaaacaaaca ggaaaacggc aaacccttta cggaataccg
     8701 tatggttgaa gttcacaagg gcgtttcgga aggtggttac acggaaataa tctttccgga
     8761 aggatttgat accagggctg ccaaggttgt tgtaaaaggg gcttataacc tgttatctgc
     8821 aaaaaagaat gcaggggaaa tggcctgttg atcctgcatc tgaattcagg atcatcgcgc
     8881 cggaatttcc ggaaatatga atcgatattg agtaatgcta tcccgacagg ccggatagca
     8941 ttacctgcaa acagggatgg caggaagagc aattattgcg gttcttcttc cagcgagcct
     9001 atcgcattgc ggagaactga ggcagaatgc ctgagctggc tgaattcttc ttcggacaaa
     9061 gggctgcgga tgatggccga cacgcccctg gccgttacga tgcagggcac gctcaggcaa
     9121 acgtcctgga tgccgaattc gccatttacc aggatggaaa ccgtcagtac gctggactgg
     9181 ttcctcagga tagctccggt aatcctgacg agcgccagcc ctaccgcaaa atgtgtggct
     9241 cccttatagt tgatgatgtg gtaagctgaa tctcgtacct gttgctcaat gagcttttgc
     9301 tggtttttcc agtcggaaca tttttcacat accgggcagt aatcgtgaat gctgatcccg
     9361 gcgatattgg tcatcgacca ggctgcaaat tcactgtcgc cgtgctcgcc caacatatag
     9421 gcatggacat tatgaacatc caccccgcag tgcatgctga gcaggtgcct gaaccgggtg
     9481 ctgtcgagaa ccgtgcctga gccgatgacg cgactcctgt cccagcccga tcgtttgacg
     9541 gcaatatagg tgagcacatc cacgggattg ctcacgatga gcatgacccc ttcacaatga
     9601 tgcgccgcta catcagcggc aatatcgccg acgacctgtg cattcttgcg cagcagttgc
     9661 agccgtgttt cgtcggggcg ctgggcgatg ccggcagtaa ttactacaag ttgagcccgg
     9721 gtgtaatcct ctggggtgcc ttccctgatg gcgacggtgg ggaagaatgg ctggccgtgc
     9781 acgaggtcaa gcacctgtcc gtgcataagg tcctcatttt tatcgatgat cacaatttcg
     9841 tcggccaacc cgctttgggc cagcgcataa cagtaagtgg cgcctaccgc acctgcgccg
     9901 acaactacca cgcggcgttg ttctgatacg tgatttgttt tcatgatgga gaaaatagta
     9961 tatgtgtttg atcctgatga aacagacagg tttcttttga taaaaagttc cgctgttttt
    10021 tgttactaag ttaacaaatc acgcattgga attgttctta tgatatgaac ggctgccggg
    10081 aaaaaaagaa tcaggcaaag gtaaaagcct gacttaatgt ctgcttttga ccatttgccc
    10141 gatttcgcta ttaggatctc ctgttccggc aattacttat gtcaaccggg aatcagggaa
    10201 atcttcagga agaaaaccta taccgaggct tcaatgttcc atacataagc ctttaagccc
    10261 tgttcttcca cttccttatt caggaaatcg agtttctcaa gcaggggctc gaccacatca
    10321 tcaggaacaa ttgtaagatg tacattgttg agcgaaggcc aggtatgggt gccatggtgg
    10381 ggatcaccct tttctgtccc ctggcctttg atgtcggtcc attgcgtata tcctctgatc
    10441 gcgagatcat ccaggatatt ctgtactcct tcggtaaggg attggttata tgctataaat
    10501 acagctttca tatttgggaa ttattgattg gctaatgtta tttcagctct ttttaacctc
    10561 ttcatttttc cgattccaaa gatggtatag atggtcggga ccaggatcag ggtgaccagc
    10621 gtggagaagg tcaggccgcc gaagatggcg atagccatag gtttccacat ttcggaaccg
    10681 ctgccggtag aaacgatcat cgggatcatg gcaaggatca tggtcagaga ggtcatgagc
    10741 acgggtctca gcctggaccg gcccgcagcg ataacggctt ctttaagctg gtaccctctg
    10801 gaggccagca ggttggtata atcaacgagg ataataccat ttttcacaac gataccgatg
    10861 agcatgacca aaccgatcat ggagatcacg ttgatggtgg aatggaaaat ccacagactg
    10921 agaaatactc cggtaagggc aaaaaggatc gagaacatga tgatgaatgg ttcacgcaat
    10981 gattcgaatt gggaggccat tacgatgtac accaggatga tcgacagcaa taataacagg
    11041 aagagatttg agaatgattc ctgcatatct tcaatgctcc cgccatattc aagcgagaca
    11101 tcatccggga tgttcatttt cgcaatttcc ctgtcaaggc ctgcctgcac agtcccgata
    11161 tcggtaccgg aaatggcggc tgaaacggta acaatacgca ctttgttctc cctttcgatg
    11221 gacgggggcg aatagaaacg tttgagcgtc gctatctcac ttagcttgat tattttgccc
    11281 tgaggggtgg caatactgat gttctcaata tcttcggtcg attgccggta tttgtcgtca
    11341 taacggacaa tcacatcgta ttcattgccg gcttctttgt atttggtggc agtcagtccg
    11401 ttgatacggt tacggaccgc gttggcgacg gtagcggtat tcagcccgaa cgaagtcatt
    11461 ttttcatggt caagcaccaa ttgcagttcg gttttatcct tatcacggct gaccttgata
    11521 tcttcggccc ctttaattcc tttcagggcg ttggagatat tttcggcaac cacgttggtt
    11581 ttatcaaaat cgttgccgta aacttttatc tccagtacgc ttccgcctcc tgaacccatt
    11641 ccgcctgacg aacgggatga tccgggatcg acatagaatt tttccacctc gggtaacact
    11701 tcgatatcct tacgcatttc ttcagcgatc tggaaaatat ccttttcacg tttctggtga
    11761 ggcgtcatct tgaaggtgta gttaatgatg taattaccgg tttcagaaaa taccgccgac
    11821 atggaattgg agccgtcgcc ggcgccggcg cttgttgatg taagctctat ttccgggtac
    11881 tttttcatga aaacgctgtc gagatagtgg gccgttttgg cggtttcctc aatccggctc
    11941 ccctgagcca gttttacctg ggccgaaatc ctgtcattat cggatgccgg cataaattcg
    12001 gtaccgatga atttgacaag taccattgag gagataaaaa tcactccggc aatgataata
    12061 attgtccacc ggtgattaac agcccagaag agtatctttt catagaaatt gtccagacgg
    12121 cggagcaatc cctgagagaa ggaatataac ttgcctgcag gcgtgtctgt cgaaggtagt
    12181 tttgcactga ggatcttgga actcagcatc ggggtgagcg tcatggaaac gatgaccgac
    12241 acggctaccg tgatcgccac aacccatccg aggggtttga agatgatacc ggtcattccg
    12301 cccagcatgg taagcgggag gaacacggca atgatggtga gcgtggaagc aataacggca
    12361 aggcttactt cgctggtgcc atagatggct gcctccctcg taaatccccc tttctcaagt
    12421 tttttggtga tgttctcgag taccacaatg gaatcgtcca ccaccaggcc gatcgcgatg
    12481 gaaagcgacg acagggtgat aatattaagg gtcccgccgg agaggaacat gtagataaac
    12541 ccggcaatca gcgatatggg gatggaaagc gagacgataa atgtcgcgcg ccatcgtcca
    12601 aggaagaaaa atactaccag cacaacgaaa atcaaagcgt agatcagcgt ctcggagagg
    12661 ttgttgatag aatcgatcgt attgaccgaa gtgtccatca gcacctgcac ctttacatcc
    12721 ggaggcagcg ttttcttgat ttcttcaagc tttttcttca cctggttggc aacagccacc
    12781 gtattggcat cggattgttt ctggatcatg atacggacgt ttttttgtcc gtttgcacgt
    12841 tcgtaggaat tgatgtcctt cagggagtcg cgcaccgagg cgatgtcctt cagatatacg
    12901 gtttttccgt tgtaattgct gacgatgata tttttaacaa catcgctgtt ttcgaattct
    12961 ccctggagcc tgagggcaag atcggctttt cccatcttca ggttaccgga cgggaggttg
    13021 aggttgttgg ctgccaggat cccgccgatc tgctcaaccg tcaggttgaa ggcatccagc
    13081 ttccgcggat caatatcgac catcacggcc ctgacggggg cgccaatctg gtaaatgctg
    13141 cctacgccgt cgatgcggtt caggggctga accagtttgt cgtccaggat atcctggatg
    13201 ccttcataac tctcgtcgca ggttacgctg aacataatca cagggatcat gctcgtgctg
    13261 attttgaaga tgaccggcgt ttccacttca tcgggcagac ggttctgggt catcccgacg
    13321 gcatccctta cttcgttggt agcttcatca aggtttgctc cccattcata ttcaagcatt
    13381 acgaccgaaa tattatcctt ggaagtggag gtcatatttt tcaggttcga gagcgatccg
    13441 aaaccatctt ccagtttacg ggtgatattt tgttccacat cggcggcatt ggcccccgga
    13501 taatacgtga tgactgaaat ataaggcgga tccatctttg ggaagaaatc caggggtaac
    13561 tgcaaatagg aatatatgcc caggattatc accccgatac aaatcatgat ggtggtgacg
    13621 ggcttcttta cggcagtgct gtataaactc attctctgaa ttgttatggc tgttacttaa
    13681 aggtgtctta tgaacgacat attatttgat gaccgagatc ggagactggt tctcaagttt
    13741 gttctggcca acgataacca cttcgtcgcc gctgttgagc ccgctcagga tctcaatctt
    13801 atcatcgaac atacgcccgg ttatcacagg cgttttaact gcaacattgt tcttgttgac
    13861 gaatacgtac atgttgttgg taccggtttg tttgatgatg gagatgttgg gaaccagaat
    13921 ggcttcccct tcgccgaggt caagcatgat cttggcaaac atgccgggac gcagtttcaa
    13981 ctggccgttc tgtatcctga cttcggccgt gaatgttttg gtggcgttgt ccatggtggg
    14041 ataaatccgg gagatatcgc cggtgaattt gatgccggga tataattccg attcaatcac
    14101 ggctttcatg ccctgcgaaa ccagcgggaa atacatggaa gaaatgctga caagggcttt
    14161 caactggctg atctgtacaa tggatacaat agccggtttg ccgactgcgg cagcgggaga
    14221 gccggagtaa atctcgccgt tttcgaaata tttgcccgaa acaattccgt cgaaaggcgc
    14281 tttgagctgc gtgttatcct gcaaaaaagc ataactggtt ttagcaacat cgtaagcggt
    14341 ttttatctga tcgtactgct ggtcggcgat gctcccggtt tttttcaggg tgtcgaacct
    14401 cctgaaatcc gattccagct tcacgaggtt aattcgggcc tgttcaagtt gcgtgcggtc
    14461 catcataacc aaagcctgtc cggcgcgtac cctgtcgctg atatttacgt tgattttatc
    14521 gatacgtccg ggtgaagccg gcgccagatg aacttcctgg taaggcagga gcgaagccgt
    14581 atattcaatg gtgcgggcta ccgattcttt gccgacaact tcggttttaa cgggaaccgt
    14641 ttgctgaacg gcagttttgg catttgccgc agcattatct ttttttgaag agcaggctgt
    14701 ctgtaaggtg actattccgg caattatgga tagaagtaaa aatttgttcg tattcatggt
    14761 atgatgatta aaggttattg ttgattttac gtaaggcaag ttctgcattt aacaactgca
    14821 gcagggtgcc ggcatggttc gattcagccg tcagatagtc gttgttggcg ctggtgagtt
    14881 ccagacttga gatcacgccc tgctgatatt tcaatttcat cttttcgagc acttctttgg
    14941 cgatttctac atttcgttgc tggttacggt attgctccag caggttgttg taattgaacc
    15001 ggagttgttt ttcctgtaag gtaagttgct gagtcagcaa ggcctgcgtg tttttattga
    15061 tatccaggtc aatcctcgcc tggcttacct gggctttcct ctggccgctg gaaaaaatcg
    15121 ggacattcag cgatagcccg agcacattgt tgggcgtcat gtcgaatttt gccgtctgga
    15181 ttttttgggt ccgggaatag taggctgcca gcgttggcag gtagcttgat tgtttcaatt
    15241 caatttgttt ctttgcaata tcgccctgca tcgagatcaa tttgaaatcc atgttgttcc
    15301 cgatatcaaa ttgcttgtcg gaagcgccat aaagtgtaca cttttcagct aattcagcaa
    15361 ggctggacga cagttggatc tcctgtcccg attccagccc cagttgaagc cgcaacaggt
    15421 tgtaacccag ttcaagttgc ctttcagttg atttgatcgc attatccact gtcgacacca
    15481 tgatggacag ttttttcggg tcggtttgtt caacaatccc ggcattggcg aggttggtgg
    15541 tcttttcgaa catcgtcagg gcattttcct tattggcttt gatgatgttt aacagctttt
    15601 cggttaccag caccgtgtaa tatgcctgaa tagtctgttc ccttacgttc agttcgtcct
    15661 tgcggtaact ttgttctgtt atggtttttg ccagcttcga aagctggacg cctacataat
    15721 aactcccgtt gaaaaccagt tgggtgacac tggctttgaa gctgctcgtc ggattgaatt
    15781 caatggatat ggattgtccc ggggccattt ctaacgaagc ttcagcccca aggtaattgg
    15841 tgtaatccat cgaagcgctt acctggggca acccctgggc aacggtttcc ctgatttttt
    15901 cggtcgattt gtctacggcc agcctcgaac cgataagcgt cctgttatga tcggtggcgt
    15961 atgtgatagc gctgtccaac gatagtacca atggctcctg tgcctgaagc gacattgtgc
    16021 ccaggcaaaa aacacagtac agcgctgctc tggaaatcct cttcatttta ttttgtttta
    16081 ttattatgta gtttttatcc tttaaacgtg gcaatagtat gatacagtat ccggacgatc
    16141 ttcaaattct tcggttcaga tatttttccc tttgaattat tgtgatcaga cggaagcatt
    16201 tccggtatat tggtttacga tggtcaaacc tttatcggtg gctatgcccc tgataaagtt
    16261 cagcatcagg gtctgaagaa tttcttcttt cgagtaccgt tctgttgaat acaattcctg
    16321 gttcttcatg acgcctgact gcgccgaaaa tatcctggca accaggtcca tgttcagttc
    16381 cttccggaac aagccttcct gcaccccttt gttgagtatt gagacaattt cagtagccat
    16441 gcaattctca cccgatttga tttttgagac attaatctcg gcaaaatact tctgaatatc
    16501 ggtaaagaac aaaggattta tcctgtttat ggcctgaatg gtgaacttca tcatgctgat
    16561 caatgcatca atggcgtttt cggaattctg aatggattca tcgaaatgcc tttctttttc
    16621 tttcatggcc atatctatac aatctctgat gagcgcatcc ttatcggaga aattctcata
    16681 gatagtcctt ttcgaaagac ccagttcctg ggcgatcata tccatggtta cggcacgaat
    16741 gccatatttt aggaataatt gattggcccc cgaaactatt ttttcacgta ccgtcatatc
    16801 gtttttttag gactgcaaaa ttagagaaaa tttcaaaaac ccaaatagtt tttaaaattt
    16861 tagtattatt taacaaatca ggcgggtggc ggaatccgga aattttaaca gtaaccggat
    16921 aagttgcaat agctctttat tttatcaatg acatttttaa attgttaaaa ttacatacca
    16981 acaatgtcat gtataacggc agatataaaa taacagcctg gacggattaa ttcagccagc
    17041 agaaaagcgt tttttgcggc cagcagttgc cggttgccat ccggctttta tgcatggatt
    17101 tctcctgaca gaatttcccg gtagatattg taattttcct ggtcgaagca gcaaaagatg
    17161 accttttcca acgaggtatt ttctgtcagg aaatgtttta cttcggcaac ggcaataccg
    17221 gcggcttcgt ttttcgggta gctgtaaaca cctgtgctga tattggggaa ggccactgtt
    17281 ttgcagccgt tttcaacggc aagcttcagg ctgttgcgat agcaggcggc gagttttacc
    17341 ggttcatcgt gtgccccccc gtgatatacc ggtcccacgg tatggataac atattttgcc
    17401 gccagccggt aaccgttggt tatcctggct tcgcccgcag ggcagcctcc cagcgtgcgg
    17461 cattcttcga gaagatgcgg gccggcagcc ctgtgaatag ctccgtcaac cccgccgcca
    17521 cccagcagtg acgaatttgc agcattcacg atggcatctg ccgtcagaac ggtaatgtcg
    17581 ccgcgtataa gttcaatcct tgcttcggtc atggctataa agaatgtatg tgatcaaatg
    17641 ccagtttatt atatggccgg cttttcgggg aagtactctt tgaccgaccg cagtaattca
    17701 tcgtgaagga tgttgttcga tgcaagcatt tctccgccga aaatataatc cttgccaccc
    17761 ctgaaatcgg ttactttgcc tccggcattc tgaacaatca acgcgccggc ggctacatcc
    17821 cagggatgaa gggcgtattc gtaaaaacct tcacaacgcc cgcaggcagt ccaggccagg
    17881 tcgatcgcgg cagttcccag ccttcgcaac ccggatgtgt tctcggcaaa gtacctgaac
    17941 aaatccatgt agcttttcat cctgccatag tcataatagg ggaaaccggt tgccagcaaa
    18001 gctttggtca tctgcgacaa gggcgaaacg gcgatagcct tcccgttcag aaaggccggg
    18061 gagcctttcc aggtataaaa gcattcgtcg aggttgggtt catacactac tcccagcacg
    18121 gtttcagcgc cgtccattaa tgcaatgctt accgagtata aaggaatgcg gtggatataa
    18181 ttggtggtgc cgtcaagcgg atcgatcacc cagttaaaac gctcgccttt tctgtctgaa
    18241 gttccttctt cggcaataaa accggcttcg ggtaaaagct gctgcaactc atttacaatc
    18301 tgtttttcgc aggttttatc cacgtaagtc acaaaattgt gcagcccttt ttcttccacg
    18361 tcggcattgc ccactttatt gatttcatct ttctggaatt gaccggtgcg ccgggaaagt
    18421 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggtttggc catcgtattc
    18541 acgattcccc agcaaccgtc gcccgagaac tgacatccca gcctcccgac ggcatatccg
    18601 atcataatcg ccggcgctgc cgcgtcaatc acataatgcc agtcgagatg gatttttttc
    18661 aggtaccaca acagggtaat cacggttacg atcagtccgc cgtaaaaggt caatccgctg
    18721 aacgacagca ggcttcccac aggattgcgg aggaaatcgc caaagttgtc gaaaatgtcg
    18781 aaaagtttgg aacctatcag agccgctacg acggcaataa tcaggatatt ccaggtataa
    18841 tcggcggggt ggatgatttc gtcaatatat tcggccgtcg tgtttttcag ctttttctgc
    18901 cggatgattt cgtatacgat tatgacggca gcagccagta tgcccagcag caggttgccg
    18961 gaggccgagg tgaggtaccg ctggggattt gccgcaaaaa cgggataatc ggtcacaatg
    19021 cctcccactt tccaggccag caaaaaagta atgattgcgc caagaagtat ttcgctggtt
    19081 gaagggggct ttgacttgag tactttccgc tgtttcggaa gcaaaagccc ttctttttct
    19141 ttgcgtttca gttcgtagta taatacggtc gccccgacca gaaatgccag ggcaaggaaa
    19201 aaaccgtacg tctggaccgg caggttccaa tgcgttccga aaagatagtt gagcatgtcg
    19261 ctgaatcgtg ggaacatagg aggagtggtt aaggtttgaa agtctgagag gttataggtt
    19321 tataaagttt caggattccg gtttgcggca gggcgtccgg agtgcgaaat gcctttatgg
    19381 ttcaaccaga acatcgcaaa tcagctcttt ttccctcgct accgataata atcttaccgg
    19441 ggtaatcgtg ttaacaagac cgggggtgta agcggtcctt acatggatat aattttctgt
    19501 ccagccgtgc atcatgccgt taacgttatc cgattcccac aggacgctga acgtttttcc
    19561 ggtatgggcc tgatagaagg caagcttttt tgcttccgac aattcatgca gtaccatgct
    19621 ccggcgatgt ttttcctttt catccacatg accttccagt tcaacggc
//
LOCUS       DAOPYV010000059        19020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_3025_length_19020_cov_12.504245, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000059 DAOPYV010000000
VERSION     DAOPYV010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19020
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3025_length_19020_cov_12.504245"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1322)
                     /locus_tag="PKI35_08490"
     CDS             complement(69..1322)
                     /locus_tag="PKI35_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44457.1"
                     /translation="MMKNPLTLLLCLTITAITVVNKPAEAQWVKVFTGNNLTCLAVVN
                     ADTVLAAGDNVMVRTFDGGATWQNIFPAGLTFNALDMEIAGNSIFACGEGKILKTTDL
                     GDTWDVVYESPTATFQCLSFAGENYGMVFFHGVTDTLMTTSDGGQNWATLPFTFSTDN
                     VECSIEMTDKTTAYIAETSYLASKLYKTIDGGLTWNLLSFPDNMGALGGPIAFTSNDR
                     GIIGSGMGVYLTKDGGSNWITIGGFPIGSYVWIYPLVSGSVYVCGFDWMAGGGPVRFT
                     NDEGATWKSQYAGNKNEDIQMADDSIGYFISGSFNTTTTEIYKTIHGGFIWGGVEAHN
                     YAGERLFELLPNPAHDRIKISSLKAGDFNNINLEISTVYGAVVINVNTAPGTYTDISG
                     LVPGLYLYRISLDNKTVQSGKIVKQ"
     gene            2170..3345
                     /locus_tag="PKI35_08495"
     CDS             2170..3345
                     /locus_tag="PKI35_08495"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNX44458.1"
                     /translation="MITFEEAFGIVALSAIELETERTELAEAAGRILAEDVFSDMDMP
                     PFNKSAVDGYACRREDLEGELTVIEVIPAGKIPEKAVGKGQCSKLMTGGMVPEGADTV
                     VMIEDIETTGENTVRFKGENTSANIAVKAEDIRKGALVLEKGIRLTPPHIAVLASVGC
                     ARPLVSRKPRVAVISTGDELVEPSQVPGIARIRNSNAYQLLAQARNAGCDAAYYGIAS
                     DNEEHTYNKITAAQLQSDIILLTGGVSTGDFDFVPAIIEKAGFDIKFRTMAVQPGKPT
                     IFAVRGKQFLFGLPGNPVSAFVQFHLLGGMLIRKMTGCTDEERILRIPMGVDYRRKKA
                     SRKSFIPVKVTTAATVLPVEYHGSAHIHSFVHADGIISIEIGQTELLKGSLVDVRLL"
     gene            3329..4129
                     /locus_tag="PKI35_08500"
     CDS             3329..4129
                     /locus_tag="PKI35_08500"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX44459.1"
                     /translation="MFDSYNRNINYLRISVTDRCNLRCVYCMPAGGVVMMKHEDILSF
                     EEILSVVKAAVGLGVNKVRITGGEPLVRKGITELVRIISGVKGIQDLSMTTNGQLLES
                     YALPLKDAGLMRVNISLDAVDPSRYSEITRGGNVGNVFKGIEAAKKAGLSPVKINCVI
                     KKSPSEPDAREVERFCRENGLEIRFIREMDLENGEFYVVQGGSGGDCARCNRLRLTAN
                     GMIKPCLFNNLEYNVRTLGVEKALQLALENKPECGTFNTGSEFYNVGG"
     gene            4173..4625
                     /gene="moaC"
                     /locus_tag="PKI35_08505"
     CDS             4173..4625
                     /gene="moaC"
                     /locus_tag="PKI35_08505"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNX44460.1"
                     /translation="MSKLSHTDSEGRAVMVDVSLKPVQLRKAVASGLISLQPGTITLI
                     RENMIKKGDVLTVAQIAGIQAAKQTPSLIPLCHPLQISKVDVELSLDEKGVNCISRVT
                     CSGQTGVEMEALTAVSTALLTVYDMCKAVDKQMTIGEIKLVEKTKTDI"
     gene            4629..5354
                     /locus_tag="PKI35_08510"
     CDS             4629..5354
                     /locus_tag="PKI35_08510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNX44461.1"
                     /translation="MVMLLLILSLAPVAALLAYIYFRDRYEKEPVALILKGFLAGAAL
                     MIPCMPVETWLSRFCTGCATIPFALYTGFIVAGTTEELLKFLMIFLLFFRNRNFNERY
                     DGIVYAVSVSLGFAAIENVMYVFNSGIPTGLIRAFTAVPAHAFFGIVMGYYFGLARFV
                     NGYRKSFLLKAFLFPMILHGLYDFILFSQNPWILIGFVPLMIILWRVGLNRIKKLQQA
                     SAFNPLSENFLELPAEEEEQQES"
     gene            5428..6351
                     /locus_tag="PKI35_08515"
     CDS             5428..6351
                     /locus_tag="PKI35_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNX44462.1"
                     /translation="MEVLSVNISEKKGTIKKPVPQIELDSRGVRGDAHAGDWHRQVSL
                     LAEESIIKFSQQAGRKINYGEFAENITTRGIEVWKTHPLDRFQIGEVELEVTQIGKEC
                     HGSSCAIFNEVGNCVMPKEGIFTRVIRPGIVKAGDTLQYLPYTFDVQIITLSDRASRG
                     EYEDLSGPRIAELAGSFFEELGWQCNIDVTVIPDDAAQLQLLLEGFVESGADFIFTTG
                     GTGIGPRDITVDVIKPMLDKELPGIMELIRYKYGSLKPNALLSRSVAGVTGGTLIYTL
                     PGSVKAVNEYCSEIFPTLKHSILMLHGIDSH"
     gene            6472..6867
                     /locus_tag="PKI35_08520"
     CDS             6472..6867
                     /locus_tag="PKI35_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44463.1"
                     /translation="MKKAVIPIVISLLLLGSAVIWVIQEQPVRAAEVLPFIVLLILVV
                     FGLYFGYRRIISHKRGEPAEDELSKGVMRKASSLSYYISIYLWLAVMYFSDKTDLETH
                     TYIGMGILGMAVVFAGCWLYYNFRGIRDA"
     gene            6860..7075
                     /locus_tag="PKI35_08525"
     CDS             6860..7075
                     /locus_tag="PKI35_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX44464.1"
                     /translation="MHNRIRELRAKQGLTQDDLARSTGVRRETIVFLEQGKYNPSLKL
                     AYDVSKVFGMKIEEIFIFEEEPGKAAG"
     gene            complement(7072..7587)
                     /locus_tag="PKI35_08530"
     CDS             complement(7072..7587)
                     /locus_tag="PKI35_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNX44465.1"
                     /translation="MEFYDVIRKRESTRNYDPMKPVGREILDRILEAGRIAPSAANYQ
                     PWHFYIVSSYSMLEKVRKCYDHPWLYDAPHILVVVGSRREAWQRPDGYNSLETDLAIA
                     MTHMILAAENEGVSTCWIAHFGYPKLRAALELKNNEEVFAITPLGYPKEGYVKRGVKN
                     RKPAEDIVSFI"
     gene            complement(7631..8059)
                     /locus_tag="PKI35_08535"
     CDS             complement(7631..8059)
                     /locus_tag="PKI35_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44466.1"
                     /translation="MGKHFRKPAVLLFLFWELPQNILGAATFLNLKRSGKTLGTSFEQ
                     GRYFIETPHRGVSLGIFIFWTKRGNRYHHENDCRMHEYGHSVQSRMLGPLYLLVVGIP
                     SAMRYFYSKWFIARHGRRWNGYYDGFPENWADRLGGITTA"
     gene            complement(8079..9374)
                     /locus_tag="PKI35_08540"
     CDS             complement(8079..9374)
                     /locus_tag="PKI35_08540"
                     /inference="COORDINATES: protein motif:HMM:NF017396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HNX44467.1"
                     /translation="MKLNRLLILLLFVLPVLTSAQDSELYKFLVSIPGAEVKKVDSSA
                     FKEFYVVMLPQLVDHNNKNGAQFKQRIYVGHAGFDRPTVIETEGYGAENINGFVVDEP
                     TKILNANQLYVEHRYFGASAPTPLDWKYLNAGQDAGDYHYIRTLFGQIYKGKWIATGV
                     SKGGQTTTEYKVFYPDDVDVSIPYVAPINYKRLDKRIDRHFSHVGTPDCRKHIRDIQD
                     YWLQNKKELLPIYEEFCTKAGFKFDLMPAEEAYDYSVLEFPFSFWQYYANCEKLPDLT
                     AANQEKMVKYLVRIVSPFWYTNAGEAFSPANYQFYTQLGYYEYNERPFRKYLKKDDYP
                     NSAFVPKGVKIEWDNSYIRKLKKFMKSGPEHMIYIYGEADPWGATAAKIKPGSGSLKM
                     VQAGGTHGAHIGSLSPEQKKEVYKALSTWLGMQLEAPVK"
     gene            9560..10120
                     /locus_tag="PKI35_08545"
     CDS             9560..10120
                     /locus_tag="PKI35_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNX44468.1"
                     /translation="MLLPRQQALDLLRQYVKNEKMIAHCLASEAVMRALAGRLGQDKD
                     AWGQAGLLHDIDVEITHADPYTHSLEGAEMLQKLGMDEDVVDAIRMHNETATGLERTT
                     LFQHALAAGETITGLITATTYVYPDRKVAAVKPSSVVKRMKEKAFAASVKRENILECE
                     MIGIPLADFAALSISAMSEIADEIGL"
     gene            10200..10520
                     /locus_tag="PKI35_08550"
     CDS             10200..10520
                     /locus_tag="PKI35_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNX44469.1"
                     /translation="MMFRNLLHKIPGFLRDKYFLTIVIFLVWIIFLDRNNLISQYRLQ
                     KELKGHKRQMEFYMQHTYDDSVALNRLMTDTAEMERIAREKYFMKRDSEDIFLIVPKP
                     DDNK"
     gene            10572..11117
                     /locus_tag="PKI35_08555"
     CDS             10572..11117
                     /locus_tag="PKI35_08555"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HNX44470.1"
                     /translation="MKKISLAFFAILITVTSVVSAQEAIPNLPVDPDTKLITYKEVVN
                     QTGSKRALFDRAVEWLPKQYVNPVDATKVRNPETGIIEIRHRFDVTYEEKGIQKSAYS
                     IDYTMRLEFKDGRYRYIISNFLMHDVSRAPVERWMNKSDKSYTPVWDQYLIQLDQEVK
                     KLIESLKQGMMPPPEKKEDIW"
     gene            complement(11283..12383)
                     /gene="mqnC"
                     /locus_tag="PKI35_08560"
     CDS             complement(11283..12383)
                     /gene="mqnC"
                     /locus_tag="PKI35_08560"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HNX44471.1"
                     /translation="MNDITGLLIKAMNGGYLAADEALFLYLEAPVTHLMVAANDVRKR
                     MHPGGKVTWIIDRNVNYTNVCVSGCKFCNFYRGVNHPEAYITTIGEYRHKIDELIALG
                     GNQLLLQGGMHPGLGLSFYTGLFRTLKDMYPRLKLHALGPPEVVHIAEMEKTGYREVL
                     EQLVAAGLDSLPGAGAEILVDRVRQEVSPNKCTAGQWLEVMHEAHNLGLTTSATMMFG
                     HVETPADRIQHLMALREVQDIRPKGTTGFISFIPWPFQDDKTVLSKRYKVKNSVSQEE
                     YIRTIAISRLALPNIQNIQASWLTVGKETGQLCLHAGANDFGSIMIEENVVSVAGANH
                     KFDQEGIQKAILEAGFTPQFRNQQFEFLDTPV"
     gene            12477..13931
                     /locus_tag="PKI35_08565"
     CDS             12477..13931
                     /locus_tag="PKI35_08565"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNX44472.1"
                     /translation="MLPFITQSKKLSAGETVIFLVNSVNDLPRDIFTTPELNYLSSSL
                     KEKDKDLASINRLGKWLFVYRVGNGKTTPKKLEKFRKAGDGLLATLNGSKIVKIVISD
                     LAARPSEALAMAEGMALGNYRFLKYVRKDVLEKEPSLKTIQIISKSLKSEQVERLNIL
                     CDAVYRCRSLVNEPLSSLNAEKLAREFERMGEEAGIKVEVMNKSRIEAMRMGGLLAVN
                     RGSADPPTFTIMEYKPDKAVNKKPLVLVGKGIVYDTGGLNLKPSAAMDGMKSDMAGAA
                     TAATVVYAIALAKLPVHVVALAPATDNRPDGNAITPGDIITMMDGTTVEVLNTDAEGR
                     LILADALSYAKNFDPMLVIDVATLTGAAMVTLGMIGIAGMHAGAGKFFNMLCESGVET
                     HERIAELPLWDEYADLLKSEIADLKNIGGRFAGTITAAKFLQHFTGYPWIHLDIAGPS
                     FLEKKDAYRTAGGTASGVRLLFEFASRLAESKQR"
     gene            14345..15229
                     /locus_tag="PKI35_08570"
     CDS             14345..15229
                     /locus_tag="PKI35_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44473.1"
                     /translation="MKRFTMLLSLLALAAIMINTGCNKDDDDPVLPTLKLSDAAGYVS
                     GDANAAYADTLHFGITADYNGTDNLVKFQLSLDGTILVDSTINTAQFTYDYKGVKNIN
                     DKEVWKFVVVDIAGNTTSDSVVITGDFGEINSYASITLGAQNNATLPGFVSYTGDTFT
                     PYTIEEAFNHQADIDMFCFYENTTSHQNMMTMAAPGSGISGIFSGDYAPEFYTVQNTT
                     YFVKTSLSASDFDAVSNDAVVLASFDPENKFRKAKVLTAGDVYAFKLQSGKFGLFKVI
                     AVTEAEDGNLEFAVKIQK"
     gene            15236..16183
                     /locus_tag="PKI35_08575"
     CDS             15236..16183
                     /locus_tag="PKI35_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44474.1"
                     /translation="MNSLFLRKHNKPHFRHGVMWLILSCMTFAGMFSSCTKEEDTSKT
                     FLMLKTGSAYTPDGAEISEGGTIRIGILASGAGSPLTYIRIDRIAGNDTVTQYDRGIY
                     CEDEGLDLDVSFSKSSAEEETWQVTVMNADRVTAVQSLKIYKGTGTAYGPIHYFPSVT
                     IGLQQNNAGNQFLDLDQGLAYNQTTVSGHEPEIDLLGYFYYTSGNPSPTFTCPGYNSA
                     IGYYPVLANWTVKNTTLYDYNSSDNNLVAPGLFDAAVNDSILVSAYNSTKVSGNCKYC
                     YTGKIIPFKTQAGKYGLIKVIRADESAEGTIEMAIKIQQ"
     gene            16207..18537
                     /locus_tag="PKI35_08580"
     CDS             16207..18537
                     /locus_tag="PKI35_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX44475.1"
                     /translation="MIRHLTFFTLLFIAGNALGQACITGRITDGKSRAAIPGVTLLVK
                     GHSGTTTDINGKFRLCNLPADTVTLELSHVSYTKAGLRLTLHSGDNDLNEIKLTPALL
                     NMDEVVITATRTDNRLIDAPGRVSLLTPRQLESIPVQNIDEVLKYTPGINYDRAFGIF
                     STKATVTMRGLSGKEQARVLVLLDGIPLNKSDGGTVDWNMVDINSVQKIEIIKGAGSS
                     LYGGNAMGGIINILTRRPDEKLSVDASLEYGTYNTMGGKFSAGKSTKLSEKGFAWYWK
                     ANMYMKQSDGYITQSEVDQQANPLYIVKSNMKEIGGMAKGGIIWNKNQRLEATFNYYN
                     DNRGTGEKYFLPEGNTTDHDSYGIMMNYLGEVKKISLRSSLYMLTENYRKVNEYMKDD
                     YTWYDVLSVRRDLGWINTASLDAGRIQKITAGIEFKNGSVDAYDEYFTSTDIVYNEGK
                     IFTSAIFAQDEIVLPGDKVKFTAGLRYDVAHYYDGSFRIETPTAETEFMQGYQVPDMA
                     SQTWSSISPRLSAQYKWKEDSRVYVQVSRGFKPSPLEDLCRSGRIKGGFKIANPSLKP
                     EYLTNFETGADAVIFNQLHFSASVYYSQGKDFQYYVTNGQTIDMGFGDRPIMIRSNIS
                     KVEIYGAETELRYNLNKNLSAFANYTYTHSVIGSYKKLTVTDTVDLSGNYLTDVPMHM
                     ASAGATWNSRFVNASVAARYTGAQYINDQNVYDELVGSDQYPAYTTIDVRVWKEFFSH
                     YTLSLNIQNLADKKYYDSKYAVCPGRFITFQVQAKF"
     gene            18556..>19020
                     /locus_tag="PKI35_08585"
     CDS             18556..>19020
                     /locus_tag="PKI35_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44476.1"
                     /translation="MRSFFALLPVCLLTAFTGFGQTNDFYDDAETKVLTKPVVEIAGE
                     VANPGPVDLSKLPLRSVIVKEDLLNNGKDKFIGAYRYDGYSLYDILNSTILKKKNEAE
                     FSPIIDLYVEVENAAGEKVIFSWGEIYYPIHRHEILIASQVMRIVPSKTKELW"
BASE COUNT         5003 a         4614 c         4582 g         4821 t
ORIGIN      
        1 aataaatggc gctcgagaat aagtggggca cgtgtcagga gacgcgagcc tggagattta
       61 ttgttatgtt attgtttgac tattttgcct gactgcacag ttttattgtc gagcgatatc
      121 cggtacaggt acagccccgg gacaaggccg ctgatgtcgg tatacgttcc cggcgctgta
      181 tttacattaa tgacaacggc gccatatact gtactgattt ccaggtttat gttgttgaag
      241 tcgccggctt tcaggctgct aatctttatc ctgtcgtgtg cggggttggg caaaagttca
      301 aacaggcgtt ccccagcgta gttatgcgct tcaacgcctc cccatataaa accgccgtga
      361 atggttttat aaatttcagt tgttgttgtg ttaaaactgc ccgagataaa atatccgatg
      421 ctgtcgtcag ccatctggat atcttcattt ttgttgccgg catactgtga tttccaggta
      481 gcgccttcat catttgtaaa gcgtaccgga cctcctccgg ccatccagtc aaagccgcaa
      541 acatatacag accctgagac aagcggataa atccacacat aactcccaat tggaaagcca
      601 cctatagtta tccagtttga tccgccatct ttagttaaat aaacgcccat cccactacct
      661 ataattcccc tgtcgttcga agtaaaagct atggggcctc cgagcgctcc catattatca
      721 ggaaacgaga gcaaattcca ggttaatccc ccgtcaatgg ttttatacag tttagaagca
      781 agataacttg tttctgctat gtatgcggtg gttttatctg tcatctcgat agaacattcc
      841 acattgtcgg tagaaaaggt aaacggaagc gtggcccagt tttgtccgcc atccgaagtt
      901 gtcatcaggg tgtcggttac gccgtggaag aataccattc cataattttc cccggcaaac
      961 gaaaggcatt ggaatgtggc cgtagggctt tcgtaaacca catcccaggt gtcgcccaga
     1021 tcggtggttt tcagtatttt accttcgccg caggcaaaaa tactgttacc ggcaatttcc
     1081 atatccagcg cgttaaaggt taacccggcc ggaaatatgt tttgccaggt ggcgcccccg
     1141 tcgaaggtac gaaccatcac attgtcgccg gcagccagca cggtgtcggc gttaaccacg
     1201 gccaggcagg taaggttgtt gccggtgaat accttcaccc attgggcttc agccggtttg
     1261 ttgaccaccg ttatggctgt aattgttaaa cataacagaa gagtaagagg atttttcatc
     1321 ataattggat ttaattggtt tcttttatga aaggcagtaa aattcagcct tgtttgaaaa
     1381 agttcattat caacatgata atcatattct tgtatcgttg atcggcatga aaggctccgg
     1441 aaaaggattg tttaattccc tgccgatgca accggttcac atgcaatggc tttctcccgt
     1501 aatggaagta ttatcacgtt ttaatgttta acggtggttc atggttcgga aacggtgaaa
     1561 ttttatcata caatccggct ttatattcgg gaacaagtaa atttctgatt ctgatatttt
     1621 tctaattata actccgacag tgaataagag aatttaatcc gtaaagagaa ttcaatcttc
     1681 ttcgctactg gttttctgtg gtaaaatatt aggctaatat aggcataatt ttttgttatc
     1741 atatttttct attatcagtt ttcttttatt gtttgttgat caaacacaaa tatccgttta
     1801 ttccatccgc ttgcgcgtct cctgacatgt gtgttgagaa ctgtcctttt ctaaccacta
     1861 aggcactgag gtcactaagt ttcactaaga atagtgtaat gctctgtttt tcttcgtgtt
     1921 ccgtgactct gtggtaaaat accctaagtg cccgtggtaa tataatctga gctgaaaaag
     1981 agaagggaac cacggagaca ttgaggtcac tgaggttcac tgagaaaatc tgcaattctc
     2041 tgtggtcctt tcgtgttctc catggctccg tggttctttc aactccttgc tccgttcggc
     2101 gcgtcaggat tattcaggga tgccttttct ttggacagta tgtttaactt tgcagagaaa
     2161 tgaggaacta tgataacatt tgaagaagct ttcggaattg ttgccctgtc ggccatcgaa
     2221 cttgaaacag aaagaactga acttgccgaa gccgccggac ggattctggc cgaagatgta
     2281 ttttcggaca tggatatgcc cccgttcaat aaatcggcgg tcgacggtta cgcctgccgg
     2341 agggaagatc ttgaaggcga gctgacggtt attgaagtga tccctgccgg gaaaataccg
     2401 gaaaaagcgg tcggtaaagg ccagtgttcc aaactgatga ccggcgggat ggttccggaa
     2461 ggcgccgaca ccgtcgtgat gatagaggat atcgaaacga ccggtgaaaa cacggtacgt
     2521 ttcaagggtg aaaacacttc tgcaaatatt gccgtcaagg cggaagatat ccgcaaaggc
     2581 gccctggtgc tcgaaaaagg tatccggctt acgcccccgc atattgccgt ccttgcctcc
     2641 gtgggctgcg ccaggccttt ggtgagccgg aaaccccgtg tcgccgtcat ctccaccggc
     2701 gatgaactgg ttgagccttc ccaggttccc ggcattgccc ggatcaggaa cagcaatgca
     2761 taccagttgc tggcgcaggc ccggaatgca ggctgcgatg ctgcctatta cggcatagct
     2821 tcagacaatg aggaacatac ctacaataaa atcacggcgg cacaattgca aagcgacatc
     2881 atcctgctga ccggcggcgt ttcgacgggc gatttcgatt ttgtgcctgc catcatcgaa
     2941 aaagcaggct tcgatataaa attccgcacc atggccgtcc aaccggggaa acccacgatt
     3001 tttgccgtca ggggaaagca attccttttc gggctgccgg gaaatcctgt ttcggcattt
     3061 gttcagttcc acctgctggg cggtatgctc atcaggaaga tgaccggatg caccgacgaa
     3121 gagcgaattc tccgcatacc gatgggcgtg gattaccgga ggaaaaaagc atcgcgaaaa
     3181 tcgttcattc ctgttaaagt tactactgcc gctaccgtat tgcctgtgga ataccatggt
     3241 tcggcccata tacactcatt tgttcatgcg gatggcatca tttccatcga aatcggtcag
     3301 acggaattat tgaaaggatc attggtcgat gttcgactcc tataaccgaa atattaatta
     3361 cctccgcata tcggtcaccg accgctgcaa cctgcgatgc gtatattgca tgcctgccgg
     3421 aggcgttgtg atgatgaaac atgaggatat tctgagtttc gaggaaattc tcagtgtggt
     3481 gaaagcagcc gtcggcctgg gcgtcaataa agtccgcatt accggaggcg agccgctggt
     3541 gcgcaaaggc attacggaac tggtcaggat tatttctgga gtaaaaggca tccaggatct
     3601 cagcatgact accaacggcc agttgctcga atcctatgct ttgccgctga aggacgcggg
     3661 gctgatgcgc gtcaatatca gcctggacgc ggttgatcct tcccgataca gtgaaatcac
     3721 ccgcggaggc aatgtgggga atgtattcaa aggcatagaa gccgcgaaaa aggccggctt
     3781 atcgcctgta aaaatcaatt gtgttataaa aaaatcgcct tccgaacccg atgcccggga
     3841 ggttgaaagg ttttgtcgcg aaaacggcct tgaaatcagg tttattcgcg agatggacct
     3901 cgaaaacggg gagttttacg tggttcaggg aggcagcggc ggcgattgcg cccgctgtaa
     3961 ccggttaagg ctgacggcaa acggcatgat aaaaccctgc ctttttaaca atctggaata
     4021 taatgtccgt accttaggtg tggaaaaagc attgcagcta gccctcgaaa acaagcctga
     4081 gtgcggcact ttcaatacag gaagcgagtt ttataatgtc gggggataac ccggattatt
     4141 ttcagatgct gttttaaaga ttctctcaat ctatgtcaaa actctcacat accgattccg
     4201 aaggcagggc cgtaatggtc gacgtcagcc tgaaacccgt tcaactgcgc aaagcggttg
     4261 cgtccggctt aataagcctc cagcccggca ccattaccct gatccgcgaa aacatgatca
     4321 aaaaaggcga tgtgctcacg gtagcgcaaa tagccggaat acaggcagca aaacaaacgc
     4381 cgtcgcttat tccgttatgc cacccgttac agatcagtaa agttgacgtg gaactttcgc
     4441 tggacgaaaa aggcgtaaac tgcatcagcc gggttacctg ttccggccag accggcgtgg
     4501 aaatggaagc cctgacggcc gtttcgacgg cgctgctcac ggtttacgac atgtgcaaag
     4561 cagtggataa gcagatgacg atcggtgaga taaaacttgt tgaaaagacc aaaaccgata
     4621 tctgatccat ggtaatgctg ttgctgatac tatccctcgc tccggttgct gccttgctgg
     4681 cctatatcta tttccgcgac aggtatgaaa aagaacctgt cgcactgatc ctgaaaggtt
     4741 ttctggcagg ggccgccctg atgatcccct gcatgcccgt cgaaacctgg ctgagccgtt
     4801 tctgtaccgg ttgcgcaacc ataccctttg ccttatatac cggctttatt gttgccggca
     4861 ctacggagga attattaaaa ttcctcatga tattcctgtt gtttttccgg aaccggaatt
     4921 ttaacgaacg ttacgacggg atcgtttacg ccgtttcagt atcgctagga tttgccgcca
     4981 tcgagaatgt gatgtatgtg ttcaacagcg gcatcccgac aggcctgatc agggcgttta
     5041 cggccgtgcc tgcccacgcg tttttcggca tcgtgatggg ctattatttc ggactggccc
     5101 gcttcgtcaa cggttacaga aaaagcttcc tgttaaaggc tttcctgttc cccatgatcc
     5161 tgcacgggct ttacgatttt atcctgtttt cgcaaaatcc ctggattctg atcggctttg
     5221 taccgctcat gattatcctc tggcgggttg gcttgaaccg gataaagaaa ttacaacagg
     5281 cttctgcctt caatccctta tcggaaaatt tcctggaatt gccggctgaa gaggaagaac
     5341 agcaggaatc ctgatagttg cgaaaacagt aaggttgtgt ttgtttaaca acaggaatga
     5401 ataatacgtt aataagaacg gaaaaagatg gaagttttat cagtcaatat atctgaaaaa
     5461 aaagggacca taaagaagcc tgttcctcaa attgagcttg attcaagggg cgtcaggggt
     5521 gatgcccatg caggcgactg gcaccgccag gtgagcctgc tggcggaaga gagcatcatc
     5581 aaattctcac aacaggcggg acgaaaaatc aattatggcg agtttgccga aaatatcacc
     5641 acccgtggta tcgaagtatg gaaaacacac ccgctcgacc gtttccaaat cggcgaggtt
     5701 gaactcgaag tcacgcagat cggtaaggaa tgccatggca gttcgtgcgc tattttcaac
     5761 gaagtaggca actgtgtgat gccgaaagag ggcattttta ccagggtgat caggcctgga
     5821 attgtaaaag cgggagatac cctgcaatac cttccatata cttttgatgt acagatcata
     5881 accctgagcg accgggcaag ccgcggcgag tatgaagacc tttcaggccc ccgtatcgca
     5941 gaactggcag gttccttttt cgaagagctc ggctggcaat gcaatatcga tgtgacggta
     6001 atccctgatg atgcagccca gcttcaactg cttttagagg gttttgttga aagcggtgcg
     6061 gatttcattt ttactaccgg cggaaccggc ataggcccgc gcgatattac ggtggatgtc
     6121 attaaaccca tgctcgacaa ggagttgccg ggcattatgg agcttatccg ctataaatac
     6181 ggaagcctga aacccaacgc gctcctttcg aggtccgtgg cgggagtaac cggcggaacg
     6241 ctgatataca cactgccggg tagcgtaaaa gccgtcaatg aatattgttc cgaaattttc
     6301 cccaccttaa agcatagtat cctcatgctt cacggtatcg attcgcattg atttccggtc
     6361 atcggtccgg ttgttggtta agctttaaac attattttac ttcctgagaa caattcgtgt
     6421 tagaaatacc taactttgtg ttagaaatat ttaacatgaa aagaataaac catgaaaaaa
     6481 gctgttattc cgatcgtgat ttctctgctg ttgttgggaa gcgcggtaat ctgggtaatt
     6541 caggaacagc ccgtcagggc agccgaagtt cttcctttca ttgtcctgct gatcctggtg
     6601 gtgttcggcc tgtatttcgg ataccggcgg ataatcagcc ataaacgggg agaaccggcc
     6661 gaagatgaat tatcgaaagg ggtgatgcgt aaagcatcct ccctgtcgta ctatatctca
     6721 atctacctgt ggctggccgt gatgtacttc agcgacaaga cggatttaga aacccatacg
     6781 tacatcggga tgggaatcct gggaatggct gtcgtttttg ccggctgctg gctatactat
     6841 aatttcaggg gaatcaggga tgcataacag gatcagggaa ttacgggcaa agcaagggct
     6901 cacccaggac gacctggccc gcagtaccgg cgtcaggcgc gaaaccattg tattccttga
     6961 acagggaaaa tataatcctt ccctgaagct ggcttacgat gtttcgaaag ttttcgggat
     7021 gaagattgaa gaaattttta tcttcgaaga agaaccagga aaggctgccg gttaaatgaa
     7081 gcttacaata tcttctgccg gtttccggtt tttgacgccc cttttcacat atccttcctt
     7141 cggatagcca aggggagtaa tggcgaatac ttcctcgttg tttttcagtt ccagcgcggc
     7201 acgcagttta ggatagccga aatgggcgat ccagcaggtg gaaacgcctt cgttttcggc
     7261 ggcaaggatc atgtgcgtca tggcaatggc caggtcggtt tccaggctgt tgtaaccgtc
     7321 gggacgttgc catgcttccc tgcggcttcc aacaaccacg agaatatgag gggcatcgta
     7381 caaccaggga tggtcatagc attttctcac tttttcgagc atggaatacg acgaaacaat
     7441 atagaaatgc catggctggt aattggcggc cgaaggagca atacgcccgg cttcgagtat
     7501 ccggtcgagg atttccctgc cgacgggttt catcggatca taattcctgg tgctttcgcg
     7561 tttcctgatg acatcgtaga attccataac taaaggtatt taaccggatt aatttttctt
     7621 gttttctcaa tcaggcggtt gttattccgc ccaaccggtc ggcccagttt tccgggaacc
     7681 cgtcataata gccgttccat cgcctgccgt gcctggcaat aaaccatttg ctgtaaaaat
     7741 atctcatcgc cgaggggatg ccgacaacca acaggtataa aggccccaac atccggcttt
     7801 gaacggaatg accgtattca tgcatcctgc agtcgttttc atgatgataa cggttccccc
     7861 gttttgtcca gaagataaaa atccccaggg agacgcccct gtgaggagtt tcgatgaaat
     7921 accttccctg ttcgaaagaa gttccgagag tttttcctga ccttttcagg ttcaggaaag
     7981 tagctgcacc gagtatattc tgaggtaatt cccagaacag gaaaagcagg actgccggtt
     8041 tccggaaatg ctttcccatg atttaagtga ggtagttact actttacagg cgcttcaagt
     8101 tgcatgccga gccatgttga aagggcctta taaacttcct ttttctgttc agggcttaac
     8161 gatccgatat gggcgccgtg tgttccgccg gcctgaacca ttttcaggct tcccgacccg
     8221 ggtttgatct ttgccgccgt tgctccccag ggatcggctt cgccgtagat gtaaatcata
     8281 tgttcaggcc cgcttttcat gaacttcttc agcttcctga tatatgaatt atcccattca
     8341 atttttacgc ccttcggcac gaaagccgag ttggggtaat catctttctt caggtatttc
     8401 ctgaacggac gttcgttgta ctcgtaatag ccaagctggg tgtagaactg gtaattggcc
     8461 gggctgaatg cctcgccggc gttggtatac cagaatgggg aaacgatgcg tacgaggtat
     8521 ttcaccatct tttcctggtt agcagccgta aggtccggca gtttttcgca gtttgcataa
     8581 tactgccaga aagagaacgg aaattcaagc actgaataat cataggcttc ttcagccggc
     8641 atcagatcga atttaaagcc ggccttggta cagaattctt cgtatatcgg gagcagttct
     8701 tttttattct gcagccagta atcctgaata tccctgatgt gtttacgaca gtcgggggta
     8761 ccgacatggc tgaaatggcg gtcgatgcgt ttgtccagcc ttttataatt aatgggcgcc
     8821 acataaggga tcgaaacgtc aacatcgtcg ggataaaaca ccttgtactc ggtagtggtc
     8881 tggccgcctt tgctcacgcc ggtagcaatc catttgccct tataaatctg cccgaagagg
     8941 gtacggatat aatgatagtc cccagcatcc tgtccggcgt tcaggtattt ccagtcgagg
     9001 ggcgttggag cggaagcgcc gaagtaccgg tgctcgacat acaattgatt ggcattcaat
     9061 atttttgttg gttcatccac aacaaaaccg ttgatgtttt cggccccgta tccttcggtc
     9121 tctatcaccg tgggacggtc gaaaccggca tggcccacat aaatccgctg tttgaactga
     9181 gcgccgtttt tattgttatg gtcgaccagt tgaggcagca taacaacata gaattcttta
     9241 aatgcggacg aatcgacttt tttaacttcc gcgccgggga tggagacaag aaatttgtaa
     9301 agttcactat cctgtgccga cgtcagtaca ggaaggacga agagtaagag aatgagcagc
     9361 ctgttgagtt tcatgatcgt atttttatac ggcaaattta tcgaaatctg atcacacaca
     9421 agtattcccg tttcttttgt tcctgtatca atgcctcata atacagttca tcgcctgatc
     9481 agcggatgca agtctgtttt aatagtttgt gaaaatgaaa aagtatgatc cttacctttg
     9541 ctgaaaagaa aattccatca tgttacttcc gcgacaacaa gcacttgatt tgctccgtca
     9601 gtatgttaaa aatgaaaaga tgatagccca ctgcctggct tcggaggctg taatgcgcgc
     9661 ccttgccggg agactcggcc aggataaaga cgcctgggga caagccggat tactgcacga
     9721 catcgatgtt gagatcaccc atgccgatcc gtacacacat agcctggaag gagcagaaat
     9781 gcttcagaaa ctgggtatgg atgaggatgt ggtggatgcc atccgcatgc ataacgaaac
     9841 ggccacaggc cttgaacgca ctactctttt ccagcacgca ctggctgccg gcgaaaccat
     9901 cacaggactt atcacagcca caacttatgt ttaccccgac cgcaaagtag cagcggtaaa
     9961 accgtcgtcg gtggtaaaaa ggatgaaaga aaaggctttt gcagcatcgg taaaacgtga
    10021 gaacatcctg gaatgcgaga tgatcggtat tccccttgcc gattttgctg cactctcgat
    10081 ttccgcaatg agcgaaatag cggatgaaat cgggctttaa ccatcaacca gtcaggctct
    10141 gctttcaaat aaatcttccg gccggcatgt agcctttttc taaactgcct atctttggca
    10201 tgatgttccg aaacttatta cataaaattc cggggtttct cagggataaa tatttcctga
    10261 cgatcgtaat attcctggtt tggatcatct tcctcgaccg caacaacctc atttcccagt
    10321 accggttgca gaaagaactg aaaggccaca agcgtcagat ggaattttat atgcagcata
    10381 cgtatgacga cagtgtcgcc cttaaccggc ttatgaccga tacggctgaa atggagcgaa
    10441 ttgcccgcga aaaatatttt atgaaacgcg acagcgaaga catcttcctc attgtaccaa
    10501 aaccggacga caataaataa cagaatcccg cggcacagaa tttgttgaat ccagatcaaa
    10561 caacacttag tatgaagaaa ataagcctgg cttttttcgc catcctgatc actgttacat
    10621 ccgttgtatc ggcccaggaa gccatcccaa accttccggt tgaccctgat acaaaactta
    10681 tcacatacaa ggaagtcgtt aaccagaccg gttcgaagag ggcgcttttc gacagggccg
    10741 tggaatggct tccgaaacag tacgtcaacc cggttgatgc taccaaagtc cgtaacccgg
    10801 aaaccgggat tatcgaaatc cgccaccgtt tcgatgtgac atacgaggaa aaaggcattc
    10861 agaagtcagc atactccatt gattatacga tgagactcga atttaaggat ggccgttacc
    10921 gctatattat ctctaacttc ctgatgcatg atgtttcgcg cgcaccggtt gaacgctgga
    10981 tgaacaaatc ggataaaagc tatacgccgg tctgggacca gtacctgatc cagcttgacc
    11041 aggaagttaa aaaactcatt gaaagcctga aacagggcat gatgccgcct cccgaaaaga
    11101 aagaggacat ctggtaacgg gagcgctgag cgaaacccag gccggttctg ctcttcaaaa
    11161 ccttaccgga cacgggatga gaaacaggcg gtggccggaa ccggaatttt aaaaacgggc
    11221 tgaatctttc cagaaagatt cagcccgttt atcatttatg cctttcaata ctgcaattct
    11281 cgttaaaccg gcgtatccag gaactcaaac tgctgattgc ggaactgcgg cgtaaacccg
    11341 gcttcaagaa tagctttctg aatgccttcc tgatcgaact tatgatttgc cccggctacc
    11401 gaaaccacgt tttcttcgat catgatggaa ccaaaatcgt tggctccggc gtgcaggcac
    11461 aattgccctg tttcctttcc gactgtaagc caggaagcct gtatgttttg gatattgggc
    11521 aatgcaagcc ggctgatggc aatcgtcctg atgtattctt cctgcgaaac agaatttttc
    11581 accttataac gtttgctcaa aaccgtttta tcgtcctgaa aaggccaggg aataaacgag
    11641 ataaagccgg ttgttccttt tggccgtatg tcctgaactt cgcgtaaagc catcaggtgt
    11701 tgtatgcgat ctgccggtgt ctccacatgg ccgaacatca tggttgccga agtggtaagg
    11761 ccaaggttgt gggcttcgtg catcacttca agccactggc cggcagtgca tttgttggga
    11821 gatacctctt gacggacacg gtcgaccaga atttcggctc ctgcacccgg caggctgtcg
    11881 aggccggcag caacaagctg ttccaatact tcacgatagc ccgtcttttc catttcggct
    11941 atatggacta cctccggcgg gcccagcgca tgaagtttta aacgggggta catatctttc
    12001 aacgtccgga acaggccggt gtagaacgat aatccgagtc cgggatgcat cccgccttgc
    12061 agcaaaagct gatttccgcc aagggctatc aactcgtcaa ttttatgcct gtattctcct
    12121 atggtcgtga tataagcttc cggatgattg actccccggt aaaagttgca gaatttacaa
    12181 cccgaaacac agacattggt ataattaaca ttccggtcga tgatccaggt cactttccct
    12241 ccgggatgca tccgtttcct tacatcgttg gcggcaacca tcaggtgcgt tacgggcgct
    12301 tccagataca gaaaaagagc ctcgtcggct gcaagatatc cgccattcat ggctttgatc
    12361 agtaacccgg ttatatcgtt catagtggtt gcaaaattat gcttttaccg taagccaacg
    12421 gactatccgt gtatctgctg catgtttgct aattttgtcc ttaaaatcca tttatcatgt
    12481 tgcctttcat cacgcagtcg aaaaaattat cagccggaga aactgtaata ttcctggtca
    12541 attccgtgaa cgaccttccc cgcgacattt ttaccacacc cgaattaaac tatttaagca
    12601 gcagtctgaa agaaaaagac aaagatctgg catccatcaa caggcttgga aaatggctat
    12661 ttgtataccg ggtggggaac ggtaaaacta cgccaaaaaa acttgaaaaa ttcaggaagg
    12721 ccggcgatgg gcttcttgca acactcaacg gaagcaagat agtcaagatc gtgatttccg
    12781 acctcgcagc caggccctca gaagcgctcg ccatggctga aggaatggcc cttggcaatt
    12841 acaggttcct gaaatacgta agaaaggatg ttttagaaaa agaaccttcg ctgaaaacca
    12901 tccaaatcat ttcgaaatca ctgaaaagtg aacaggttga aaggctcaac atcctttgcg
    12961 acgccgttta tcgttgccga tcactcgtga atgaaccgct ttcgtcgctt aatgccgaaa
    13021 aactggcccg ggaatttgaa cgtatgggcg aagaggcggg cataaaagtg gaagtgatga
    13081 ataaaagccg catcgaagcg atgcgaatgg gcggactgct ggccgttaac cgcggaagcg
    13141 ccgatccccc tactttcacc atcatggaat acaaacccga caaagcggtc aataaaaagc
    13201 ctctggtact ggttgggaaa gggattgttt acgataccgg cggcctcaac ctgaaaccgt
    13261 cggcggctat ggacggtatg aagagcgaca tggccggcgc cgctaccgca gcaaccgtcg
    13321 tttatgccat tgctttagcc aagcttcctg ttcatgttgt tgccctggcg ccggccaccg
    13381 ataaccggcc cgatggcaat gccatcaccc cgggcgatat catcactatg atggacggca
    13441 ccacggtgga agtgctcaat accgatgccg aagggagact gatactggcc gatgcccttt
    13501 cctatgccaa aaattttgat ccgatgctgg tgattgatgt ggccacgctt accggggctg
    13561 ccatggttac tctggggatg atagggattg ccggaatgca tgccggggcg gggaaatttt
    13621 ttaatatgct gtgcgaatcg ggggttgaaa ctcacgaacg gattgccgaa ttacccctgt
    13681 gggacgaata cgcagacctc cttaaatcgg agattgccga cctgaaaaac atcggcggcc
    13741 ggtttgccgg aaccatcacg gcagccaaat tcctccagca ttttaccggt tatccctgga
    13801 tacaccttga tatcgcagga ccctcatttc tggagaaaaa agatgcgtac cggaccgctg
    13861 gcgggacagc ttcgggagta agactcctgt tcgaatttgc ctcacgcctg gctgaatcaa
    13921 aacaaagata aaagtccgga ttttcaatcc ccgggcggca cgtatgcgtg cggtaatctt
    13981 catacccgtc agcctgaatt aacgggcatt atccattcat cagccatgca ccgcaggaaa
    14041 atgagcatct ccctgagccg acggcaatcc aaatccctat ggttgccagc ctcaaaaagg
    14101 aagaccatat tgcagccatt ccggccggca tccgggctaa ggcttttcct gcatccggtc
    14161 ccttgatttc aaaaagctga aaaaggagcc ggaacccggt taatttacct gaagaatttc
    14221 agatcttcgg gattgtaagc ttacaacact cacaattttg aacttaattt atattttttt
    14281 gaagcaatgt catacatttg ccgctgatat gaatctattt tctaatcgaa aaaacaaaaa
    14341 aaagatgaaa cgttttacca tgttattgtc cctgttggct cttgctgcca tcatgatcaa
    14401 caccggatgc aacaaagatg atgatgaccc tgttttacca accctgaaac tgtcggatgc
    14461 cgccggatat gtttccggtg acgcaaacgc cgcttatgcc gatacgctcc attttggcat
    14521 taccgctgat tataacggca ctgataacct ggtaaaattc cagttgtcgc tcgacggaac
    14581 catcctggtc gactccacga tcaataccgc ccagttcact tacgattata agggcgtaaa
    14641 aaacattaac gataaagaag tctggaaatt tgtcgttgtt gacattgccg gaaataccac
    14701 ttccgattct gtcgttatca ccggcgactt cggcgaaatc aattcttacg cttccattac
    14761 cctcggggca cagaacaatg ccactttacc gggatttgtt tcctataccg gcgacacttt
    14821 cactccgtac acgattgagg aagcgtttaa ccaccaggcc gacattgaca tgttctgctt
    14881 ttacgaaaac accaccagcc atcagaatat gatgacaatg gctgctcccg gatcgggcat
    14941 ctccggcatc tttagcggtg attatgctcc tgaattttac accgttcaaa acactaccta
    15001 cttcgtaaaa acttcactgt cggcaagtga ttttgacgcc gtaagcaacg acgctgttgt
    15061 acttgcatcc tttgatcctg aaaataaatt caggaaagcc aaagtgctca ctgccggtga
    15121 tgtttatgct ttcaaacttc aatcgggcaa gtttggcctt tttaaagtta ttgccgtgac
    15181 ggaagcagaa gacggaaacc tggaatttgc tgtgaaaatt cagaaataag ccggaatgaa
    15241 ttcattgttt ctccggaagc acaataagcc acatttccgc catggtgtaa tgtggcttat
    15301 tctttcctgc atgacctttg caggaatgtt ttcctcctgt accaaagagg aggatacctc
    15361 gaaaaccttt ctgatgttaa aaaccggttc ggcctacact cctgacggag ccgaaatttc
    15421 cgaaggagga acgatcagga tcggcatcct ggcatcgggc gccggttcac cgcttacgta
    15481 tatccggatc gaccgtattg ccggaaatga taccgtgact caatatgacc ggggcattta
    15541 ttgtgaagat gaaggactgg atctcgacgt cagcttcagc aaatcatcgg cagaggaaga
    15601 aacctggcag gtaaccgtga tgaatgccga tcgtgtgacc gctgttcagt ccctgaaaat
    15661 ttacaaagga accggaacag catacggtcc gatccattat ttcccgtcgg taacgatagg
    15721 ccttcagcaa aacaatgccg gcaaccagtt cctggatctc gatcagggcc ttgcttacaa
    15781 tcagactacc gtcagcggtc atgaaccgga gatagatttg ctgggctatt tttattacac
    15841 atccggaaac ccatcgccga cgtttacctg tccggggtat aattccgcca tcggttatta
    15901 tccggtcctg gccaactgga cggtaaaaaa cacgaccctt tacgattata attcgtctga
    15961 caataacctg gttgcgcccg ggctgttcga tgcagccgtc aacgacagca tcctggtttc
    16021 ggcttataac tcgacgaagg taagcggcaa ctgcaaatac tgttataccg gcaagatcat
    16081 cccatttaaa acacaggcag gaaaatacgg gctcatcaaa gtgatccggg ctgatgaatc
    16141 tgccgaaggc actattgaga tggccatcaa aattcaacaa taatgtcaat ccgcgatatt
    16201 tctcccatga ttcgccattt aacttttttc accctgttat tcatagcagg gaatgcgctg
    16261 ggacaggcct gtataaccgg aagaataacg gacggcaaga gccgggccgc cattccgggc
    16321 gtcacgttat tggtgaaagg tcactccgga accacgaccg atatcaacgg caaattcagg
    16381 ttgtgcaacc tccctgccga tacggtaacc ctggaactca gccatgtttc ctatacaaag
    16441 gccggcctgc gcctcacttt acattccgga gataatgacc tgaacgaaat aaaacttact
    16501 cccgcccttc ttaacatgga cgaggtggtg atcacagcca cacgtaccga taaccggctg
    16561 attgatgctc ccggccgtgt gagtctgctt acgccgcgcc agttggagag cattcccgtt
    16621 caaaacatcg acgaggtgct caaatatacg ccgggcatca actacgaccg cgctttcggg
    16681 atattcagca ccaaagcaac ggtaaccatg cgcggcctca gcggcaaaga acaggccagg
    16741 gtactcgtgc tgctggacgg aatacccctg aataaatcgg atggcggcac ggtggactgg
    16801 aacatggttg atataaattc ggtacagaaa atcgaaatca ttaaaggtgc aggatcatcc
    16861 ctgtatggcg gcaacgccat gggcgggatc atcaacatcc tcacccgccg gcccgatgaa
    16921 aagctttcgg ttgatgcttc gctggaatac ggcacctata acaccatggg cgggaaattc
    16981 agcgccggaa aatcaacgaa gctatccgaa aaaggttttg cctggtattg gaaagccaat
    17041 atgtacatga agcagagcga tggctacatc acccagtcgg aagttgatca acaggcaaat
    17101 ccgttgtata tcgttaaatc gaacatgaaa gaaatcgggg gtatggcaaa gggcggcata
    17161 atatggaata aaaatcagcg cctcgaagcc accttcaact attacaatga caatcgcggc
    17221 actggggaaa aatatttcct gcccgaaggt aatactacgg atcacgattc gtatggcatc
    17281 atgatgaatt acctgggaga ggtgaaaaag atcagcctga ggtcgtcgtt gtatatgctt
    17341 accgaaaact acaggaaggt taacgagtac atgaaagacg actacacgtg gtacgacgtt
    17401 ctttcggtaa ggcgcgacct gggctggatc aatacagcct cgttggatgc cgggcgcatt
    17461 cagaaaatca cggccggcat tgaattcaag aacggcagtg tggacgccta tgacgagtat
    17521 tttacctcga cggatattgt ttataacgaa gggaaaattt ttacctctgc catcttcgcc
    17581 caggacgaaa tcgttctgcc gggcgacaag gtaaaattca cggcaggcct gcgctacgat
    17641 gtagctcatt attatgacgg ttcgttccgc atcgaaaccc ctaccgcaga aaccgaattc
    17701 atgcaggggt accaggtccc cgatatggca tcccaaacat ggtcgtcgat aagcccgagg
    17761 ctttcggcac agtataaatg gaaggaagat tcacgtgtct atgtccaggt atcaagaggc
    17821 ttcaagcctt cgccgcttga ggacctctgc cgttcagggc gcatcaaggg cggattcaaa
    17881 atcgccaatc cgtcgctgaa acctgaatac ctgaccaatt tcgaaaccgg tgcggatgcc
    17941 gtcattttca atcagttgca cttctcggct tccgtttatt attcacaggg gaaagacttt
    18001 cagtactatg taacaaacgg ccagaccatc gacatgggct tcggcgaccg tcccatcatg
    18061 atccggtcca acatcagcaa agtggaaatc tatggtgcgg aaaccgaatt acgttacaac
    18121 ctcaacaaga atctttcggc ctttgccaat tacacctaca cccattcggt tatcggttcc
    18181 tataaaaaac ttacagtaac cgatacggtt gacctctcgg ggaattacct tactgatgtc
    18241 ccgatgcaca tggcctccgc aggggccacc tggaacagca ggttcgtaaa cgcttccgtt
    18301 gccgcacggt acaccggcgc tcagtatatc aacgaccaga acgtttatga tgaacttgta
    18361 ggttccgatc aatacccggc ctatacgacc atcgatgtaa gggtttggaa ggagtttttc
    18421 tcccattaca ccctcagcct gaacattcag aacctggccg ataaaaagta ctacgatagt
    18481 aaatatgccg tttgcccggg acgatttatc accttccagg tgcaggcaaa attttgataa
    18541 tcaaaacaaa ctgatatgag atcttttttt gctttgttgc ccgtttgcct tttaacagcc
    18601 ttcaccggct ttggacagac caacgatttt tatgatgacg ccgaaaccaa ggtgcttacg
    18661 aaacctgttg ttgaaattgc cggcgaagta gccaatccgg gaccggtcga cctttcaaaa
    18721 ctgccgcttc gttcggtgat cgtgaaggaa gacctgctga ataacgggaa agataaattc
    18781 atcggcgctt accgttatga cggatactca ttgtacgaca tcctgaactc aaccatcctg
    18841 aagaagaaaa acgaagcaga gttctccccc attattgacc tttatgttga agtggaaaac
    18901 gccgccggcg aaaaggttat tttcagttgg ggtgagattt attacccgat ccaccgtcac
    18961 gagatcctga tcgcatcaca ggtaatgcgc attgttccgt caaaaaccaa ggaactctgg
//
LOCUS       DAOPYV010000060        18696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_3096_length_18696_cov_11.124350, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000060 DAOPYV010000000
VERSION     DAOPYV010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18696
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3096_length_18696_cov_11.124350"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..2831
                     /locus_tag="PKI35_08590"
     CDS             198..2831
                     /locus_tag="PKI35_08590"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNX44477.1"
                     /translation="MEIPSKYNPTTIESKWYSYWLERKFFRSVPDEREPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDVLVRRARMQGKNACWVPGTDHASIATEAKVVAKLKEEGIEK
                     YSLTREEFLAHAWEWREKHGGIILEQLKKLGASCDWDRTCFTMDEARYESVINVFIDL
                     YNKGLIYRGVRMVNWDPKALTALSDEEVIYKELQSKLYYVRYRVEGAEDEWVTIATTR
                     PETILGDTAVCYHPEDERFAHLKGKRVLVPLLNRSIPMISDEYVDREFGTGCLKITPA
                     HDVNDYMIGQKYQLESIDIFNDNGTLSPAAQMYVGEDRFTVREKIVDDLKAAGNLVKI
                     EDYVNKVGFSERTDEVIEPKLSMQWFLRMPELAKPALDVVMNDTVKFHPDKFKNTYRY
                     WMENVRDWCISRQLWWGQRIPAYYLPTGQVVVAKSAEEALELARTRFNKPELQLKDLR
                     QDEDVLDTWFSSWLWPISVFDGIRHPDNEDMKYYYPTDVLITAPEILFFWVARMIMAG
                     IEYQKGIPFRHVYLTGIVRDKLGRKMSKSLGNSPDPISLMEKYGADGVRVGMLLTSPA
                     GNDLPFDEALCEQGRNFSNKVWNALRLVKGWTVDDTIPQPAYAAASVRWFEARWNDTL
                     SVIDNLYDDYRLSEALMTVYKLTWDDFCSWYLEMIKPGFQQPIDRATYESTVSFFEEL
                     MQLMHPFMPFITEEIWHRLRKREEGDCIMVSLLPPRREFEAAILDKFSVAVEVVMALR
                     TIRKEKNIPFKEPMDLMIRKNFDEQPDLTFDSVVEKLCNLSPIRYVDEKVNGALTFVI
                     KSTEFYVPLGSKLDTEAEIAKLTQELEYTKGFLKSVMKKLSNERFVANAKPEVVEIER
                     KKQADAEARIQVLEEQIAALK"
     gene            2943..3692
                     /locus_tag="PKI35_08595"
     CDS             2943..3692
                     /locus_tag="PKI35_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44478.1"
                     /translation="MKMQLRLFLGVAGFILLVSNYTTAQTLEFVNKDNSYYLRFAKRG
                     TIKDGVQVGKWLNVNFNNIVYKEYYFDEFGKPVGTWKLFYPNGSLRKEMEYENNRVVR
                     YTRFSMDSVKCFELITNEGFSDSLYFKIENHEEDIFDAEGRVSEIIFRNLNGRVESKR
                     VFIYDYYEGVDDLIKILNAEKFTGTIDVWNVNKDHWKKNIFKEGNYRSIVDSYKQGVL
                     QYQKEYDENGKKARITKFDEKGNVVYEKSYN"
     gene            3852..4082
                     /locus_tag="PKI35_08600"
     CDS             3852..4082
                     /locus_tag="PKI35_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44479.1"
                     /translation="MLDKVYGITINVKPNPACEWAASDYTLPDDVSSAEPKLTNRMGI
                     TVEICTINGQKGQNLIDTRKLPPGTYIYSLTT"
     gene            4214..5041
                     /locus_tag="PKI35_08605"
     CDS             4214..5041
                     /locus_tag="PKI35_08605"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44480.1"
                     /translation="MNKIITILLLIPIRLMAQIPNAGLENWVLINGCEEPTGWVTNNL
                     APWSISVEKTNDRWIDEWGMRVRSNNNIGFEGPMPGIVSTKFLFAGEYNRLSFYYKVV
                     SALPPAYAEIAIYEKLLNGSFNLLAKADFTETDCIWHYMSLPFYVSMSTDSLKIVISA
                     NTVSSGTYFQGYSEWIIDDLKLKYTDVGVKEPSADKVLINPNPAKDDFTLSIPSSWIN
                     LSTDLIIIDNTGRKVMSRKIETSTSVINVASLSTGLYIVMLSNNQFQLSMKLVILKE"
     gene            complement(5166..5813)
                     /locus_tag="PKI35_08610"
     CDS             complement(5166..5813)
                     /locus_tag="PKI35_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNX44481.1"
                     /translation="MKRYITFFLTLLSMTAGAQSFQIALLKYNGGGDWYANPTSLTNL
                     ISFCNKNLNTAIAKDYATVEAGSAELFDYPFVHITGHGNIIFNDQEAQNLRNYLLAGG
                     FLHISDNYGIDRFIRPQMKKVFPELEFVELPFSHPIYHQKYNFPNGLPKIHEHDDKAP
                     RGFGLMWEGRLICFYDYECDLGDGWEDPEVHKDSPEIRLKALQMGANIIQYVFTK"
     gene            complement(5816..6529)
                     /locus_tag="PKI35_08615"
     CDS             complement(5816..6529)
                     /locus_tag="PKI35_08615"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNX44482.1"
                     /translation="MQLFYSPDIVAETAGQSILLDAEESHHCIRVLRKKTGDTIHITD
                     GRGRLFEAVVTNENSGKCLLMVTSMVSAAARMPFHLHLAVAPTKNNDRFEWFLEKATE
                     IGISEITPVICEHSERTVLRTDRMQKILVSAMKQSLNLHLPALNEPVKLAGFLKKEFT
                     GQKFIGYVEEKQEVLLKDRYQKGSDCLILIGPEGDFSQAELEAAKEKGFLAISLGNSR
                     LRTETAALVATMTINLLNG"
     gene            complement(6603..10280)
                     /locus_tag="PKI35_08620"
     CDS             complement(6603..10280)
                     /locus_tag="PKI35_08620"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX44483.1"
                     /translation="MKKYLRLLLPALFLLAGASVYSQEKQAAPADTSQYPYWIQMMQD
                     PGANFFQTQRAFELYWQGRPITRGCGWKPFKRWENFMRTRVSPQGVKPAPDAVSNAYR
                     QYMQQHSQSRSLNGDWVNQGPFSIPTAKGYQGLGRINAIAFHPANPDIIYIGAPSGGL
                     WVTTMGGNNWSTSTDMLPTLGVSSIAINPSNPQIIYIGTGDRDAGDAPGLGIMKTTDG
                     GVNWDMANTGMGNVVVGKLLIDPENTDILYAATSSGLYKTVNAGANWSLKASGNFKEL
                     VFKPGDYSTLYASADARFYRSVNHGENWTQITSGIPSASRGVIGVSPANPQIVYFLLT
                     QSDNGFKGIYRSTDGGLNFTTRSTSPNIMDWSCDGSGSGGQAWYDLCIAVDPTNADIV
                     YSGGVDIWKSTNGGTNWQINGHWYGGCSVPSVHADHHVFEVSPLNNRIYVGCDGGIYW
                     TDNGGTNWNEITSGLPISQTYKLGQSATVDDRVVNGYQDNGSSLFADGSWYAIGGGDG
                     MECAIDYTNHMVSYTTIYYGSIDRHYGTNNQGAIAGNGVNGIDEEGAWVTPFIIDEKD
                     PNTMFIGYKNIWRSRNIKNTSASGVKWKKISTINTGNLSVLEQSPVNTNLLYAASGGS
                     LYISINAQAEDPAWADISTFLPSSSEITDLEAHPFEENTVYLTQANNKIYKSTDRGLS
                     WTDITGTLPNIHMSTIVYYKQSQEGLYLGTDAGVFYKDQSMADWIPFNNGMPANARVM
                     ELEIYYDSVNPANDRIKAATYGRGLWKSDLYFTTPVADFAADKTIVPPGCPVNFTDLS
                     LGVPTQWDWAFVGGTPSSSNEKNPQGIVFNTPGTYNIELTSSNSAGSGSILKTSYITV
                     SDTLKPIAGFSASQRVFCSAGETVTFTDQTEFCPNSWQWEITPNTVTYVNGTSMHSQS
                     PQVMFNQEGNYTVQLVVSNNNGMDVVVKEKYIIVGGYYLPFTEDFETKQFGTRSWEIE
                     NPDMNITWDIDTVYANGSSQYAARMDFFHYQAPPGRRDRMISPALNFTGMHNVNLQFD
                     HAYATRYASTSDSLIIYISDDCGSNWTRVFQAGEKGQGTFATTPKTTSLYVPSIVDDW
                     CGIGWGSNCYTIDLSPWANKTGIKIAFESFNRYGNNLYIDNITVGEEAGIQTPAAVAG
                     EISVFPNPASGAVTLTSDKPLGKTTISIVDLAGVTRLKKVVNAKHPLQETLSLKEIKA
                     GLYVIKISTDDTFYARKLIIN"
     assembly_gap    10298..10397
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10545..11336)
                     /locus_tag="PKI35_08625"
     CDS             complement(10545..11336)
                     /locus_tag="PKI35_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNX44484.1"
                     /translation="MKVKVKHAWFLAALAIVLPLYSAMAGNGIPPAPVPPRLVNDMAG
                     ILEPSQVNELERKLVAFSDSTSTQIVVVTVSSLEGYDISDFTQRLGQSWGVGSKKYNN
                     GCVILVKPKTGTESGEADIETGYGLEHLITDGDAWNIIQHEMIPHFKQNDYYGGIDAA
                     ADAVIKFTTGEYKSDTYGKGAGTPALIIIFIVIAVVFILSRFNNNRHHTIGRRSGMGP
                     FVFWPGSSGGDWGGFTGGSGGLGGGGGFGGFGGGGFGGGGASGSW"
     gene            complement(11382..11828)
                     /locus_tag="PKI35_08630"
     CDS             complement(11382..11828)
                     /locus_tag="PKI35_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNX44485.1"
                     /translation="MKLTARSFFSPEQKEDLKLAIQNAELDTSGEVRIHLETSCKGEA
                     NDRAVQIFKTLGMDKTELRNGVLFYLAVKNRKFAVIGDSGINRMVDENFWEHLKALLI
                     ENFAEGKFTEGLILGVSNVGEELKKYFPHKPDDTNELPDELSFGDE"
     gene            complement(11844..12449)
                     /locus_tag="PKI35_08635"
     CDS             complement(11844..12449)
                     /locus_tag="PKI35_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNX44486.1"
                     /translation="MRKPIIIIGSLILLAIILITWMSGVYNGLVNYEQKVNKSWADVQ
                     GAYQRRADLIPNLVATVKQYANYEQQTLIKVIEARAEATKTDINIDPKNLNETTLAKF
                     QESQGQLTSDLSKLLVIVEQYPQLKANENFMQLQNELERTENRINVERRNFNDAVQQY
                     DTKIKRFPTNLLADMFGFKYKAYFEADKGSENAPKVDELFK"
     gene            complement(12493..14403)
                     /gene="htpG"
                     /locus_tag="PKI35_08640"
     CDS             complement(12493..14403)
                     /gene="htpG"
                     /locus_tag="PKI35_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNX44487.1"
                     /translation="MTLQTGKINVQTENIFPIIKKFLYSDHEIFLRELISNAVDATIK
                     LRTLASMGKYQEDLGDVTMEVSLDPKKKTLTVKDKGIGMTADEVEKYINQIAFSGAEE
                     FLKKYKGKTEVEQSGLIGHFGLGFYSSFMVSDKVEIRTKTYKKGAGSKSVRWECDGSP
                     EYKLEECDKTDRGTEIILHISDDSKEFLEEFRVLDLLKKYCKFLPVPIRFGNEKTWEA
                     SETEKDDKGEPKQVEVEKPRIINNTNPLWKQMPAGLTDEDYKKFYRELYPHTFEEPLF
                     NIHLNVDYPFNLTGILFFPKVKRNFEVQRDKIQLYCNQVFVTDSVEGIVPDFLTLLHG
                     VLDSPDIPLNVSRSYLQSDQNVKKISGHIMKKVADRLEEIFKNNREEFEKKWDDIKLF
                     IEYGMVSEPKFYERAEKFFLFKDTEGKFYTFEEYKKKIQDAQTDKEKSLVYLYTTNPE
                     DQHGFITAATGRGYNVLVMDTPLDTHFVNALEQKFEKSHFSRVDADVIDKLIAKEDQL
                     PSKLTEDQQKQITAVFETLVDKNRFHVVFESLSENDMPVIITQPEFMRRMKDMSAIGG
                     GYEFMNNMPDNYNMVVNPNHRLVGQLAETSDEPARDRLAKQLIDLALLSQNLLKGSEL
                     TSFIQRSVDIIQ"
     gene            15306..16322
                     /locus_tag="PKI35_08645"
     CDS             15306..16322
                     /locus_tag="PKI35_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNX44488.1"
                     /translation="MRNSAGDGKHCFMPNVSRLRITGYKNLIHSLSSKQETMDDQPKL
                     ERMLNLLLTLSGSRNFTLAQLSERFSMSERTLMRYLATFRNVGFIVECRDGAYSIPRV
                     EKPFKSIGDLLHFSEEEAYILTNAIHSIDDNNLLKTNLVNKLYSLYNFDRVAETVVKP
                     ENISTVHNLISAIREKKQVLLRRYRSSHGNLVRDRLVEPFDFTTNYQFVWAFEPESRC
                     CKLFKTSRIGEVQALDKGFENEAYHEKEPMDVFRISSKEQVRVILRLSLRAYNLLLEE
                     YPLAEKYVTATDDNLWQFDGQVCGFEGVGRFVMGLCDETEVVEPEELKDYLRKKIKNF
                     IPKI"
     gene            16389..16796
                     /locus_tag="PKI35_08650"
     CDS             16389..16796
                     /locus_tag="PKI35_08650"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HNX44489.1"
                     /translation="MEFFKSVILGGAGLMAFPGSADATQENKQKLKLIDAYIAGFQYY
                     DGPEVADMLETGLKLTLRREPQNRYDHSAVEVYAGEAKLGYLPYRDNITIALLMDAGI
                     RAHACITELDPESFPYGSVKMEVWCEQDALSRA"
     gene            16943..17752
                     /gene="cas6"
                     /locus_tag="PKI35_08655"
     CDS             16943..17752
                     /gene="cas6"
                     /locus_tag="PKI35_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HNX44490.1"
                     /translation="MRFKLIYTIASSGNNTIPLNYQYEFSSWIYRTIHYADPGFARWL
                     HDEGYTAGRQHFKFFTFSNLEIPRRAFSIAGDRMRILSDECSMQISFLIGEAASPFIR
                     GLFLNQDFTIGDKISQVRFRVKSVERMPDPLFGNEMTFRALSPVVIGKSRAPEGGSGK
                     AYLSPEADGYGPLFIQNLACKVLVSKTRDKISPAELEACRFELLGKPKKKGILIKAHT
                     PQQTKVIGYQFRFRITAPADWLAVGYDAGFGEENSEGMGCAGIDDNERREK"
     gene            17887..18273
                     /locus_tag="PKI35_08660"
     CDS             17887..18273
                     /locus_tag="PKI35_08660"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNX44491.1"
                     /translation="MAIKFKVIEKGQPGVAGGGEKKFYASTVTNGELTLSGLTRLIEK
                     SSTVSGADIRAVLYAMVDVMVDSMANGQIVRLGELGSLRVSLNSEGKATADEVNSSAI
                     KNTRVIFSPGKEIKDMLKTLEFAKGN"
     gene            18437..>18696
                     /locus_tag="PKI35_08665"
     CDS             18437..>18696
                     /locus_tag="PKI35_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44492.1"
                     /translation="MSLDTVLQIGKVLRSSENSLKYFKYIEACPKDKDGNWPICITIP
                     VKEDFSFDWEGMKITPENQRDSLFYLKFKTSDSDGLVKYVFG"
BASE COUNT         4778 a         4279 c         4702 g         4837 t
ORIGIN      
        1 catctagctt atagccgttg gctccttgca ccaagcacca tgctccatgc tcctctcgtt
       61 ctccgttctc cgttttcctt tttccgttct acccctgcat tccggcaact ctgcaactct
      121 gaaactccga aactttcaat cttttcccat ctttccttac ctttgcacac tttatcagaa
      181 actgcgaaca cttcattatg gaaatcccaa gcaagtacaa tcctacgacg attgagagca
      241 aatggtacag ctattggtta gagcgtaaat ttttccgttc cgttcccgat gaacgcgaac
      301 cctacaccat tgtcattccg cctcccaacg tcaccggagt cctgcacatg gggcatatgc
      361 ttaacaacac cattcaggat gtgctggtcc gccgcgcccg catgcaggga aagaacgcct
      421 gctgggttcc cggcaccgac catgcttcca tagccaccga ggccaaggtg gttgccaaac
      481 ttaaggaaga aggcatcgaa aaatactcgc ttacccgtga ggaatttttg gcccatgcct
      541 gggaatggag agaaaaacac ggcggcatca tccttgaaca gcttaaaaag ctgggcgctt
      601 cctgcgactg ggaccgcacc tgcttcacca tggatgaagc tcgttacgag tcggtgatca
      661 atgtcttcat cgacttgtac aacaaagggc tcatttatcg cggcgtgcgc atggtaaact
      721 gggacccgaa ggccttgacc gccctttcgg atgaagaagt catctacaag gaactgcagt
      781 caaaactcta ctatgtccgt taccgggtcg aaggcgccga agacgaatgg gtgaccattg
      841 caaccacccg tcccgaaacc atattgggcg acacggctgt ttgttaccat cccgaagacg
      901 aacgttttgc tcacctgaaa ggcaaacggg tgctggttcc gttgctgaac cgcagcattc
      961 ccatgatcag cgatgaatac gtggaccgtg aattcggtac gggatgcctt aaaattacgc
     1021 cggcgcacga cgtgaacgat tacatgatcg gccagaagta ccagctcgaa tccatcgata
     1081 tcttcaacga taacggaacc ctgagccccg cggcacagat gtacgtgggc gaggaccgct
     1141 tcaccgtcag ggagaagatc gtcgatgacc tgaaagcagc cggcaatctc gtgaagattg
     1201 aggactatgt gaacaaggta ggcttcagcg aacgcaccga cgaagtaata gaaccgaaac
     1261 tctcgatgca gtggttcctc cgcatgcctg agctggcaaa gccggcgctg gatgtggtga
     1321 tgaacgatac ggtgaaattt catcccgaca aattcaagaa tacctatcgt tactggatgg
     1381 aaaacgtccg cgactggtgc atcagccgtc agttatggtg gggacagcgc ataccggcct
     1441 actaccttcc aaccggacag gttgtggtcg caaagtccgc agaagaagcg ctggaactgg
     1501 cgcgtaccag gttcaataaa ccggaactgc aactcaagga tctccgccag gatgaagacg
     1561 tgctcgatac ctggttctcg tcgtggctgt ggccgatttc cgttttcgac ggtatccgcc
     1621 atccggacaa tgaggatatg aagtattatt atccaaccga tgttctgatc accgcgcctg
     1681 aaatactctt cttctgggtg gcccgtatga tcatggcagg gattgagtat cagaagggaa
     1741 tacctttccg gcatgtttat ctgacgggaa ttgtccgcga caaacttgga cgcaagatga
     1801 gcaaatcgct tggcaactct cccgatccca tcagcctgat ggaaaaatac ggcgccgatg
     1861 gcgtgcgggt aggcatgctg ctcacatcgc cggccggcaa cgacctgcct ttcgatgaag
     1921 ccttgtgcga acagggacgc aacttcagca acaaggtgtg gaatgccctg cgcctggtga
     1981 aaggctggac agtggacgat accattcctc agcccgctta tgccgccgct tccgtccggt
     2041 ggttcgaagc ccgctggaat gataccttat cggtcatcga caacctttac gatgactatc
     2101 gcctgagcga agcgctgatg acggtttaca agcttacctg ggacgatttc tgttcatggt
     2161 acctcgagat gatcaaaccc ggcttccagc agcctatcga cagggctacc tatgaaagca
     2221 ccgtcagctt tttcgaagaa ctgatgcaac tcatgcaccc gttcatgccg tttatcaccg
     2281 aagaaatctg gcatcggttg cggaagcgcg aagaaggcga ctgtatcatg gtttcattgc
     2341 ttcctccgcg cagggaattc gaagccgcca ttcttgataa attttctgtt gccgtagaag
     2401 tggtaatggc gctgcgaacc atacgcaagg agaaaaatat accgttcaag gaaccgatgg
     2461 atctgatgat ccgcaagaat ttcgacgaac agccggatct tactttcgac agcgttgtgg
     2521 agaaattatg caacctgagc cctatccggt atgtggatga aaaagtaaac ggcgccctga
     2581 cattcgttat caaatctacc gagttttatg ttccgctggg aagcaaactc gacaccgaag
     2641 ccgaaattgc caaacttacc caggaactgg aatataccaa aggcttcctg aagagtgtga
     2701 tgaagaaact ctcgaacgag cgcttcgtgg ccaacgctaa acccgaagtg gttgaaattg
     2761 aacgcaaaaa gcaggctgat gccgaggcaa gaattcaggt gctggaagag cagattgcgg
     2821 cgttgaaata gtaatctgtg gtttaatatc ctttaattgc attttttttc ttcgtttgtt
     2881 agaagaataa ttatgatatt tgcacagttc tgacaaaaga ctgatttcaa atatttataa
     2941 ttatgaaaat gcaattaaga ctcttcttag gagtcgccgg ttttatacta ttagtttcaa
     3001 actatacaac agcacaaacg ctggaatttg ttaataaaga caattcgtac tatctgcggt
     3061 ttgcaaaacg cggtacgata aaggatggtg tgcaggtggg gaagtggtta aatgtcaatt
     3121 tcaataatat tgtatataaa gaatactatt ttgatgaatt tgggaagcct gtcggaacct
     3181 ggaaattatt ttatccaaat ggcagtctga gaaaagagat ggaatatgaa aacaatcgtg
     3241 ttgtaagata tacaaggttt tcaatggata gcgttaaatg ttttgagcta atcacgaatg
     3301 aaggatttag cgatagtctg tattttaaga ttgaaaacca tgaagaagat atttttgatg
     3361 ctgaaggaag ggtctcagaa ataatattta gaaatttgaa tggcagagta gagtcaaaac
     3421 gtgtttttat atatgattac tacgaaggag tagatgatct gataaaaatc ctgaatgctg
     3481 aaaaatttac cggcacgatt gatgtttgga atgtaaacaa agatcactgg aagaaaaata
     3541 tattcaaaga aggcaattac agatctattg tcgattcata taagcaaggt gtccttcaat
     3601 atcagaaaga gtatgacgaa aatggtaaaa aagcaagaat tacaaaattt gatgagaagg
     3661 gcaatgttgt ttacgaaaaa tcctataatt gatttgtccg gttcaaatat ttttttgatt
     3721 aatccgcatt gttagattat ctttgagtta ataaagtacc cgaagtattt ggattaatgt
     3781 taaagtattt atgccagtgc ctgcaaacag acggcgagat tttttataaa agtacaagag
     3841 tttcccccgg tttgcttgac aaggtttatg gtataacaat taatgtaaaa cccaatcccg
     3901 catgtgaatg ggctgcttcc gattataccc tgcccgacga tgtttcatct gccgaaccaa
     3961 aactcacaaa ccgcatggga ataactgtgg aaatttgtac aattaacgga cagaagggac
     4021 aaaatctcat agatacacgc aaacttcccc ctggtactta catttattct ctcactacct
     4081 gaaacaggca gttaaacggg taactatcaa tcttaaggaa atgactaaga tcattcatga
     4141 ctgtgcatat ttgcctccat tctacccctt gcattttagc ctgtaatgtt aaccttccta
     4201 aaaacgaaat ccgatgaata aaataataac aatcttactt ctaattccaa ttcgtctgat
     4261 ggcacagata ccaaatgccg gccttgaaaa ctgggtgctt atcaatggat gtgaagaacc
     4321 tacaggatgg gtaacaaata atttagcgcc atggagcatt tcggttgaaa aaaccaatga
     4381 tcgctggatt gatgaatggg gtatgagagt ccggagtaat aataacatag gttttgaagg
     4441 gccaatgcca ggtattgttt caacaaagtt tctttttgcc ggagagtata accgactatc
     4501 cttttattac aaagttgttt ctgctttacc acctgcgtat gcagaaattg ctatttatga
     4561 gaaattgcta aatggaagtt ttaatttgct tgcaaaagct gattttacag aaacggattg
     4621 tatttggcat tatatgtcat tgccttttta tgtatccatg tcaactgact cgctcaaaat
     4681 agtcatcagt gccaacacag tctcaagcgg cacatatttt cagggatatt ctgagtggat
     4741 tattgacgat cttaaattaa agtacacgga tgttggcgtc aaagaacctt cagctgacaa
     4801 agtactgatt aacccaaatc cggcgaaaga cgattttact cttagcattc cgagcagttg
     4861 gattaattta tcaaccgatt taattatcat cgataataca ggcaggaaag taatgagccg
     4921 caaaatcgaa acctcaacat ccgtcataaa tgtagcgagt ttatctacag gattatatat
     4981 tgtaatgctt tctaataatc agtttcaatt gagtatgaag ctagtcatat taaaagaatg
     5041 atcactcttc attcaaactt tttggagagt gagagccgtg ggacagctat ctcgataaca
     5101 ggcagggatt cttgcttatt ttttgaactg gatattcggc aatccggcac cagcaacctc
     5161 attcattact tcgtaaacac atactgaatg atattcgctc ccatctgtaa ggctttcagc
     5221 cttatctcgg gcgagtcctt atgaacttcc gggtcttccc agccatcgcc caggtcgcac
     5281 tcataatcgt aaaagcaaat cagccgtcct tcccacatca gcccgaatcc cctgggagcc
     5341 ttgtcgtcat gttcgtgtat tttcggcaac ccgttgggga agttatactt ctgatgataa
     5401 atgggatgac tgaagggcag ttccacgaat tcgagttcgg ggaaaacctt tttcatctgc
     5461 ggcctgataa acctgtcgat gccgtaattg tcggagatat gcaggaatcc gccggccagc
     5521 aggtagttgc gaaggttctg cgcttcctgg tcgttgaaaa tgatgttgcc gtgcccggtg
     5581 atgtggacga acggataatc aaacagttcg gcgctgccgg cttccacggt ggcgtaatct
     5641 ttggctatgg cggtattcag gtttttattg cagaaactga taaggttggt gagcgatgtg
     5701 gggttggcgt accagtcgcc gccgccgtta tacttcagca gcgcaatctg gaacgattgc
     5761 gctccggcgg tcatcgataa aagcgtaagg aaaaaagtga tgtatcgttt catgtttatc
     5821 cgttcaataa gttgatggtc atggtggcga ccagggccgc cgtctcggtt cttaaacggg
     5881 aatttcccag gctgatggcg agaaaccctt tttcttttgc cgcttcaagt tccgcctggc
     5941 tgaaatcgcc ttccgggccg atgagaatca ggcagtcgga gcctttttga taacgatctt
     6001 tcagcagaac ttcctgcttt tcttccacat agccgatgaa tttctgtccg gtgaattcct
     6061 ttttcaggaa gccggccagt ttaaccggct cattcaatgc cggcagatgc aggttcagcg
     6121 attgtttcat ggccgaaacc aggattttct gcatacggtc ggtacggaga acggttcgtt
     6181 ccgagtgttc gcagatcacc ggggtgattt cactgatgcc gatttctgtc gctttctcaa
     6241 gaaaccactc aaaccggtcg ttgttcttgg tgggagccac ggcaagatgg agatgaaacg
     6301 gcatccgtgc ggcggcggaa accattgagg tgaccatcag caaacatttc ccggaatttt
     6361 catttgtcac aacggcttca aacagccggc ctctcccgtc ggtgatatgg atggtgtcgc
     6421 cggttttttt ccgcaatacc cggatgcaat gatgcgattc ctccgcatca agcaggatgg
     6481 attgtccggc ggtttccgca acgatatcgg gtgaataaaa aagctgcatg gcttattgtt
     6541 gccccaaaat taaaaaacct gcccgatata tgggcaggtt tttatcagag tgccggaagg
     6601 cgttagttaa tgatgagttt acgggcatag aaagtatcgt ccgtgctgat ctttataaca
     6661 taaagaccgg ccttgatctc ttttaaacta agtgtttcct gaagggggtg ttttgcgttg
     6721 acaacctttt tcaaacgggt gacgccggcc agatccacga tcgaaatagt ggttttgccc
     6781 aacggtttat cggaggtaag ggtgacggcg ccggaggccg gattcgggaa caccgagatt
     6841 tcgccggcga cagcggccgg tgtttggatt cctgcttcct ccccaacggt gatgttgtcg
     6901 atatacaggt tgttgccgta acggttgaac gactcaaagg cgatctttat ccccgtcttg
     6961 ttggcccagg ggctgaggtc gatggtatag caattgcttc cccagccaat gccacaccag
     7021 tcgtcgacaa tggaaggaac atagagggac gttgtttttg gcgtagtggc aaaggttccc
     7081 tggccttttt ctccggcctg gaataccctt gtccagtttg acccgcaatc gtccgaaata
     7141 tagatgatca gtgaatcgga cgtggatgcg taacgggtgg cataggcatg gtcgaattgg
     7201 aggttgacat tgtgcatgcc ggtgaagttc agcgccgggg aaatcatcct gtcacgacgg
     7261 ccgggagggg cctggtaatg gaagaaatcc atccgcgctg cgtactggct tgatccgttc
     7321 gcgtaaacgg tgtcgatatc ccaggtaatg ttcatgtccg gattctcaat ttcccagctc
     7381 cttgttccga actgcttcgt ttcgaaatct tccgtgaacg ggagataata tccgccgaca
     7441 ataatatatt tctcttttac aaccacatcc atgccgttgt tgttcgacac caccagttgt
     7501 accgtgtaat tgccttcctg gttaaacata acctgagggc tttgagaatg catcgacgtt
     7561 ccgttgacat aggttacggt attgggtgtg atttcccatt gccacgaatt ggggcagaat
     7621 tccgtttggt cggtaaaggt caccgtttcg cctgctgagc agaaaaccct ctggctggct
     7681 gaaaaaccgg caatgggttt cagggtatcg ctgaccgtga tataggaagt tttgaggatg
     7741 cttcccgatc cggccgaatt ggatgaggtg agttcaatgt tataggtgcc gggcgtgttg
     7801 aaaacgatac cctgcgggtt cttttcgttt gaggaggatg gagttccgcc gacaaatgcc
     7861 cagtcccatt gtgtgggaac gccgagcgaa aggtcagtga agtttacagg gcacccggga
     7921 ggcacaatgg ttttatccgc tgcaaaatcg gcaacagggg ttgtaaagta aaggtccgac
     7981 ttccacaaac cgcgtccgta agtggcggct ttaatgcggt cgttggcggg attaaccgaa
     8041 tcatagtata tttcaagctc cataacgcgg gcattggccg gcattccatt gttgaaaggg
     8101 atccagtcgg ccatcgactg gtccttgtaa aacacaccgg catctgttcc gaggtacagg
     8161 ccttcctggc tttgtttgta atacacgatg gtgctcatgt gaatattggg cagcgtacct
     8221 gtgatatcgg tccaggaaag ccccctgtcg gtcgatttat agatcttgtt gttcgcctgg
     8281 gttaaataaa cggtattttc ctcgaaagga tgggcttcca ggtcggttat ttcggaagag
     8341 gacgggagga atgttgaaat atcggcccat gcagggtctt cagcttgggc gttgatgctg
     8401 atgtacaggc tgccgccgct ggctgcatac aggaggttgg tattaaccgg cgattgctcc
     8461 agcactgaca gattacccgt gttgatggtt gagattttct tccatttcac gccgcttgcc
     8521 gaagtgttct tgatgttacg gctacgccag atgtttttat acccgataaa catggtattc
     8581 ggatcttttt cgtcgatgat aaaaggggta acccaggcgc cttcttcatc gatgccgtta
     8641 acgccgttgc cggcaatggc gccctggtta ttggttccgt aatgtctgtc gattgatccg
     8701 tagtaaatag tggtgtaact aaccatgtgg ttggtgtagt caatggcgca ttccatgccg
     8761 tcgccaccgc ctatggcgta ccaggagccg tcggcaaaca gggaggagcc gttatcctgg
     8821 taaccgttta caacacggtc gtcaacagta gcgctctgac cgagtttata ggtctggctg
     8881 atcggcaggc cgctggtgat ctcgttccag tttgtgcctc cgttgtcggt ccagtaaata
     8941 ccgccgtcac atccaacgta gatgcggtta ttcagcgggc tgacttcaaa tacgtggtgg
     9001 tcggcgtgta ccgatggcac gctgcatccg ccataccagt ggccgttaat ctgccagttg
     9061 gtgccgccgt ttgttgattt ccagatgtcg acgccgccgg agtatacgat gtcggcattt
     9121 gtcgggtcaa cggcgatgca aaggtcatac caggcctgtc cgcctgatcc ggaaccgtcg
     9181 cacgaccagt ccataatatt gggggaagtt gaacgggtag tgaagttcag gccgccgtcg
     9241 gttgaacgat aaattccttt aaagccgtta tcgctttgag tcagaaggaa ataaacaatc
     9301 tggggattgg ccggggatac gccgatcaca ccgcgtgatg cagacgggat gccggaagtg
     9361 atttgtgtcc agttttctcc gtggtttaca gaacgataga acctggcgtc tgccgaagca
     9421 tacaaggttg aataatcacc gggtttgaag acaagttcct tgaaattgcc cgaggctttc
     9481 agcgaccagt tggcgcctgc atttacggtt ttatataatc cgcttgaagt ggcggcatat
     9541 agtatatccg tgttttccgg gtcaatgagc agctttccga cgacaacatt acccatcccg
     9601 gtgttggcca tgtcccagtt tactccgcca tcggtggttt tcataatgcc taatccggga
     9661 gcgtcgccgg catcccggtc gccggtacca atgtatataa tctggggatt ggacggatta
     9721 atggcgatgg atgaaacgcc gagggtgggc agcatgtccg tggaagtgga ccagttgttg
     9781 ccccccatcg tggtaaccca taaaccgcca gaaggcgctc cgatgtaaat aatatcagga
     9841 ttggcagggt ggaaggcgat agcgttgata cgtccgaggc cctggtaacc tttggcagta
     9901 ggaatcgaga aaggtccctg gttaacccag tcgccgttca gcgaccttga ctggctgtgc
     9961 tgctgcatgt attgccggta tgcgttagat accgcatccg gagcaggctt aaccccctgg
    10021 ggcgagaccc tggtcctcat gaaattttcc cagcgtttga acggcttcca tccgcatccg
    10081 cgggtgatcg ggcgtccctg ccagtaaagt tcaaaagcgc gctgtgtttg aaagaagttg
    10141 gcgccgggat cctgcatcat ctgtatccag tacggatatt gtgacgtatc ggcaggagcc
    10201 gcctgctttt cctgtgaata aacggatgcc cctgccagta gaaacagggc cggtaacaaa
    10261 aggcgtaggt attttttcat gaatcagaat tttgctgnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnaac agggccggta acaaaaggcg taggtatttt ttcatgaatc
    10441 agaattttgc tggtgtgcaa atgtactgat ttcatgcaat tataaaagaa accagggaac
    10501 ctgatttaat atatattgta gaggggatgt tccggggaag ctgcttacca gcttccgctg
    10561 gctccgccgc cgccaaagcc gccgcctccg aagcctccga aaccgccgcc accgcccaag
    10621 ccgccgctgc cgccggtaaa tccgccccag tctcctcccg atgatccggg ccagaaaaca
    10681 aaggggccca tcccgctcct gcgtccgatg gtgtggtgcc ggttgttgtt gaaccggctg
    10741 aggatgaaaa caacggctat gacaataaag attatgataa gggcaggcgt tccggcgcct
    10801 tttccgtagg tgtcggattt atactcgccg gtggtgaatt ttattacggc atcagcggca
    10861 gcatcaatac cgccgtaata atcgttctgt ttgaagtgcg gaatcatctc atgctggatg
    10921 atattccagg catcgccatc agtgatgaga tgttcgaggc cgtaaccggt ctcaatatcg
    10981 gcttcgccac tttcggtgcc ggttttgggc tttaccagga ttacacagcc gttgttatac
    11041 ttttttgatc ctacgcccca ggattgtccg agccgctgcg tgaaatcgga aatatcgtat
    11101 ccttcaagcg atgataccgt gacgacaaca atctgcgttg aggtggaatc gctgaaagcg
    11161 accagcttac gttcaagttc atttacctgt gaaggctcca atattccggc catgtcgtta
    11221 accagcctcg gcggaacagg agccggggga ataccatttc ccgccatcgc ggaatagagc
    11281 ggcagtacaa tggccagcgc cgccaggaac catgcgtgtt ttacttttac cttcatccgg
    11341 agaatgtttg agattgcaaa ttattaacca gccggaccgg tctactcatc tccaaatgat
    11401 agttcatcgg gaagttcatt tgtgtcgtca ggcttatggg gaaaatattt tttaagttct
    11461 tctcccacgt tggaaacccc gagaatcaat ccttcagtaa attttccttc ggcaaagttc
    11521 tcaatcaaca aggccttaag atgttcccaa aagttttcat ccaccattct gttgatgccc
    11581 gaatcgccga tcactgcaaa ttttctgttt ttaacggcca gataaaacag gactccattc
    11641 cggagttctg tcttatccat gcccagggtc ttgaatattt gcacagcccg gtcattcgct
    11701 tcgcccttgc aactggtttc aaggtgtatg cgcacttcgc ctgaggtgtc gagttcggca
    11761 ttctgaatag ccagtttcag atcttccttt tgctcaggcg agaaaaaaga acgcgcggta
    11821 agtttcattt ctccggtgtg gtattattta aacagttcat caactttcgg agcattttct
    11881 gatcctttgt cggcttcgaa atatgccttg tatttaaagc cgaacatatc ggccagcagg
    11941 ttggtcggga agcgcttgat tttggtgtca tattgctgga ccgcatcgtt gaaattccgg
    12001 cgctcgacat tgatacggtt ttcggtgcgt tccagttcat tctgcaactg catgaagttc
    12061 tcgttggctt tgagctgcgg gtattgttcc acgatcacca gtaatttcga cagatcggac
    12121 gtcaattgtc cctggctttc ctggaacttc gccagggtgg tttcgttcag gtttttggga
    12181 tcgatattga tgtccgtttt cgtggcttcg gcgcgggctt caattacttt gatcagggtt
    12241 tgctgttcat aattggcata ctgttttacg gtagccacca ggtttgggat caggtcggca
    12301 cggcgctggt atgcgccctg cacatcggcc catgatttat tcaccttttg ctcgtaatta
    12361 acgagcccgt tatacacgcc cgacatccag gtaataagga tgatggcaag gaggataagg
    12421 gatccgataa tgattattgg ttttctcatc tggttacttg attttgtggt tgtcagatca
    12481 aagttcgtct atttattgaa tgatgtcaac actgcgctga ataaagcttg tcagttcact
    12541 gcctttcaga aggttctgcg ataacagggc caggtcgatg agctgttttg ccagccggtc
    12601 gcgcgcgggt tcatcgcttg tttcggccag ttgccccacc agacggtggt tcggatttac
    12661 caccatgttg taattgtcgg gcatgttatt catgaattcg tagccaccgc cgatggccga
    12721 catgtctttc atacggcgca taaattcggg ctgggtgatg ataacaggca tatcgttttc
    12781 gctcaggctt tcgaaaacca catgaaaacg gtttttatca accagcgtct cgaaaacggc
    12841 ggtgatctgc ttttgctggt cttcggtaag ttttgacggg agttggtctt ctttcgcgat
    12901 aagcttgtcg attacatcgg catcaacgcg cgagaagtgc gatttttcaa atttctgctc
    12961 caacgcattc acaaaatggg tgtcgagcgg cgtatccatc accagcacat tgtatccgcg
    13021 gcctgttgct gcagtgataa agccatgctg atcttcaggg ttggtggtgt acaggtaaac
    13081 aaggcttttc tccttatcgg tctgcgcgtc ctgtatcttc tttttatatt cttcaaaggt
    13141 gtaaaatttg ccttcggtat ccttgaagag gaagaacttc tcggcgcgtt cgtaaaattt
    13201 gggttctgaa accatcccgt attcaatgaa gagcttgatg tcgtcccatt tcttttcgaa
    13261 ctcttcgcgg ttgtttttaa agatttcttc cagcctgtcg gcgactttct tcatgatatg
    13321 ccccgaaatc ttcttcacgt tctggtcgct ctgcaggtaa ctgcgcgata cattcagcgg
    13381 gatatccggc gaatcgagca cgccgtgcag caatgtaagg aagtcgggaa cgatgccttc
    13441 caccgaatcg gtcacaaaga cctggttaca gtataactgg atcttatcgc gctgcacttc
    13501 aaagttgcgt ttcactttgg ggaagaacag gatgcccgtc aggttgaagg gataatccac
    13561 gttcaggtga atgttgaaca gcggttcctc gaaggtgtgc gggtaaagtt cacggtaaaa
    13621 ttttttgtaa tcttcgtcgg tgaggcccgc cggcatttgt ttccacaggg gattggtatt
    13681 gttgatgatc cgcggctttt caacttccac ctgtttgggt tcgcctttgt cgtctttttc
    13741 ggtttccgag gcttcccacg ttttttcgtt tccgaaacgg atgggaacgg ggaggaattt
    13801 acaatacttc ttcagcaggt cgaggaccct gaattcttcc aggaattctt ttgaatcgtc
    13861 cgaaatatga aggatgatct cggtgccgcg atcggtttta tcgcattctt ccagtttgta
    13921 ttcgggtgag ccgtcgcact cccaacggac acttttgctg ccggcccctt ttttgtaggt
    13981 cttggtcctg atttcaacct tgtcggaaac cataaacgaa gaataaaaac caagcccgaa
    14041 atggccgatc aggccgcttt gctcaacttc ggtcttgcct ttgtattttt tcaggaattc
    14101 ctcggcgccc gaaaatgcaa tctggttgat atatttttca acttcatcgg ctgtcatgcc
    14161 gatacccttg tcttttaccg taagggtttt ctttttcgga tccagggata cttccatagt
    14221 gacgtcgccc aggtcttcct ggtacttgcc catcgaggca agcgtcctta gtttgatggt
    14281 tgcatctact gcattggaaa tgagctcacg cagaaaaatt tcgtgatcag agtaaaggaa
    14341 tttcttaata atgggaaaaa tgttctctgt ctgaacattg attttaccgg tttgtagagt
    14401 catggttatg aaattttgcc atgaaccgaa caaagtgtgt gccaccggaa aggatgtgac
    14461 aatttggcac gggcgagggg cggggagcag ggagcaaggg gcaggagcct gaacatatat
    14521 ttcggtaagg aggggagacc gtttcgtcgt ttaaagccct gcgggcttaa aactcctcgc
    14581 ggaatagtaa tggtaaggta gtctttggag atggggtgaa gccaagagct aaaagctaac
    14641 ggccaaaagc aaggggcagg gagcagggag cgaggggcag gagcctgaac atatatttcg
    14701 gtaaggaggg gagaccgttt cgtcgtttaa agcccggagg gcttaaaact cctcgcggaa
    14761 tagtaatggt aaggtagtct ttggagatgg ggcgaagcca agagccaaaa gccaggggca
    14821 aggagcagga cggaaaacgc aaaattaaga acgcaaaacg caaaccttcg ctgccaggca
    14881 agcctgaccc tttacataca agccttcccg ggcaggcagg cctgccacct tacgcgcagc
    14941 cttcccgggc aggcaggtct gaaacacgga aacactgaaa cactgcccac gaattccacg
    15001 aatgtcaatc acgaattcca cgaaatccgt tatcctcaca acatttattc gggtccattc
    15061 gtgcttcatt cgcgtccatt cgtggataaa aaataatcac gaattcaacg aatgtcaatc
    15121 acaacttcca caaattacga atcacaatta acgaatcacg aattacgaaa aacgaaactc
    15181 aaaatgcaga acgcaaaatt aagaacgcag aacgaccagc ggccaacggc caaaagccag
    15241 gggcaagggc gccagggtgc agggagccag ccctgaacgt actttgaaaa attcctccta
    15301 cttttatgag aaattccgcc ggcgacggaa agcattgctt catgccgaat gtcagccggt
    15361 taaggataac cgggtataaa aaccttattc attccctctc atcgaaacaa gaaactatgg
    15421 acgaccagcc gaaacttgaa cgaatgctta acctcctcct caccttgtcg ggaagcagaa
    15481 atttcaccct tgcgcaattg tcggaacgtt tcagcatgtc ggagaggacg ctgatgcgct
    15541 acctggccac cttccgcaat gtggggttta ttgtagaatg ccgcgacggc gcatacagca
    15601 tcccgagagt tgaaaagccc ttcaaatcca tcggcgacct gctccatttc tcggaagagg
    15661 aagcttacat ccttaccaac gcgatacatt ccatcgacga caacaacctg ctgaaaacca
    15721 accttgtcaa caaactttac tcgttatata atttcgaccg ggttgccgaa accgtcgtga
    15781 agcctgaaaa catcagcacc gtgcataacc tgatcagcgc gatacgggag aaaaaacagg
    15841 tgttgctgcg gcggtacagg tcgtcgcacg gaaacctggt gcgcgaccgt ctggtggaac
    15901 ccttcgactt caccaccaac taccagtttg tctgggcctt tgaacccgaa agccgttgct
    15961 gcaaattgtt taaaacctcg cggatagggg aggtgcaggc gctggacaaa ggttttgaaa
    16021 acgaggcata tcacgaaaag gaacccatgg atgtgttccg catcagttcg aaggaacagg
    16081 tacgcgtgat acttcgcctg agcctgaggg catacaacct gcttctcgaa gaatatcccc
    16141 tggccgagaa gtacgtcacg gccacagatg acaacttgtg gcagtttgac gggcaggttt
    16201 gcgggttcga aggagtggga cgtttcgtca tggggctatg cgatgaaacg gaagtggttg
    16261 aaccggaaga acttaaagat tatctcagga aaaaaataaa aaatttcatc cctaaaatat
    16321 aactgacaga agttgtcata tgccgtatct aattttgagc tttcaaacaa ttgaaagcta
    16381 tgaacagaat ggaatttttc aaaagcgtga tcctgggcgg ggccggattg atggcttttc
    16441 ccggaagcgc tgatgctact caggaaaaca aacaaaagct aaagctgatt gatgcttaca
    16501 tcgcaggttt ccagtattac gacggccccg aggtagccga tatgcttgaa accgggctta
    16561 aactcacact tcgccgcgaa ccgcagaacc gctacgacca cagcgccgtt gaagtgtatg
    16621 ccggcgaagc aaaacttggc taccttcctt accgggataa cataactatc gctttgctga
    16681 tggatgccgg catcagggcc catgcctgca taacggaact cgatccggag agtttcccat
    16741 acgggagcgt gaagatggag gtttggtgtg agcaagatgc cttaagcagg gcatagcgcc
    16801 ggccggatga tcgtcaggta acccgccatc attgtttaag tattatgaac caaaagggga
    16861 taaaataaat ttagaaaaaa attagtaata attgaatcag gaatggacaa atttatttat
    16921 atattagcga agaatttgta tcatgcgttt taaacttatc tatacgattg catcatccgg
    16981 gaataatact attccgctga attaccagta cgaattctcc tcctggatat atcgcaccat
    17041 tcattatgcc gatcccggat ttgcccgctg gctgcatgac gaagggtaca ccgccggcag
    17101 gcagcatttc aagtttttca ctttctcaaa cctggaaatt ccgcgcaggg cgttcagcat
    17161 tgccggcgac aggatgagaa tcctttcgga cgaatgctcc atgcagatat cttttctcat
    17221 cggggaagcc gcttcgcctt tcatccgggg ccttttcctg aaccaggatt tcaccatcgg
    17281 cgataagata agccaggtga ggttcagggt gaaatccgtc gaaaggatgc ccgatccgct
    17341 ttttggtaac gagatgacat tcagggccct gtcgccggtt gtgatcggga agtcgcgggc
    17401 gcctgaaggg ggcagcggca aggcttacct gtcgccagaa gcggatggtt acgggccgtt
    17461 gtttattcag aacctggcct gtaaagtgtt ggtgagcaaa acacgggata agatttcgcc
    17521 tgccgagctg gaagcctgcc ggtttgaact tctcggcaag ccgaaaaaaa aaggtatatt
    17581 gataaaagcg cacaccccgc agcaaaccaa ggttatcggg tatcagttca ggttcaggat
    17641 caccgcccct gccgactggc tcgccgtagg atatgatgcc gggtttggcg aggaaaacag
    17701 cgaagggatg gggtgcgccg ggattgatga taatgagcgc cgggagaaat gaaacgaatc
    17761 cttagtacgg aggattgcaa aagccgaaga gacctgattg ccggtgcgta tgattgaaag
    17821 caaggtaatg tttagcatat agttaacgcc ttcgcaggca aatgtttaac acttaattca
    17881 atcattatgg ctattaaatt taaagtaatt gaaaaaggcc agcccggcgt tgccggtggc
    17941 ggcgaaaaaa agttctatgc ttcgaccgtg acaaacggcg agctaaccct cagcggactg
    18001 acccggctga tagaaaaatc cagcacggta agcggcgccg atatccgggc cgtgttgtat
    18061 gcgatggttg atgttatggt cgattcgatg gccaacgggc aaattgtacg tctgggcgaa
    18121 ctggggagtc tgcgtgtgag cctcaacagc gagggaaaag ccacggccga cgaggtaaac
    18181 tcgtcagcca ttaaaaacac gagggtgatt ttcagccccg gaaaggaaat taaggatatg
    18241 ctgaagaccc tggaatttgc gaaaggaaat tgatccttgg gatgggtgca ggattttgtg
    18301 ccgtggaaaa aaaatttcca cggcacaaaa ttaaatttcc acggcaggaa attggggacg
    18361 gcacggcagg gaattgggat gatggggaaa tacaataaat aaaggatatg aaacaaacgt
    18421 acaaggcgat tataatatga gtttagacac agtacttcaa ataggcaaag tcctccgcag
    18481 ttcggaaaac agcctgaaat attttaaata tatagaggct tgccctaaag acaaagatgg
    18541 caattggcct atatgtatta ccattcctgt aaaagaagat ttctcttttg attgggaggg
    18601 aatgaaaatt acgccagaaa atcaaagaga ttctctcttt tatctgaaat tcaagacttc
    18661 agatagtgac ggcttagtga aatatgtttt tggaga
//
LOCUS       DAOPYV010000061        18664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_3102_length_18664_cov_11.498146, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000061 DAOPYV010000000
VERSION     DAOPYV010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18664
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3102_length_18664_cov_11.498146"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..333
                     /locus_tag="PKI35_08670"
     CDS             196..333
                     /locus_tag="PKI35_08670"
                     /inference="COORDINATES: protein motif:HMM:NF040113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HNX44493.1"
                     /translation="MIESKLIAVIAVLATVFIGLAVYLFLLDRKIGKLENQLKERREK
                     R"
     gene            330..761
                     /locus_tag="PKI35_08675"
     CDS             330..761
                     /locus_tag="PKI35_08675"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNX44494.1"
                     /translation="MKKVHVIVILAVMVALGVVISLFTGSETYSDFSAAASGASKQYQ
                     ISGRLNVDKPILYNPDEDANTFSFYMADEKGRECKVIYRGGKPRDFEKSEQIVITGNM
                     ENDSVFLAGNLLLKCPSKYNDEKRPARFNNKEFRSSGETAD"
     gene            complement(840..1409)
                     /locus_tag="PKI35_08680"
     CDS             complement(840..1409)
                     /locus_tag="PKI35_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44495.1"
                     /translation="MKKILALAILGISLWGCSGSNQKEANKAGDQAKDQNTVVISNDL
                     ENALSVIPSWSNEKTVVKMEGAPAHSGQYVTLVDDKFVYSYAFREFIDNIDQRIPKKV
                     TVKGWVYSTVASPDLSIIMDISENNSPIDWKAYSLTSTVKEANSWIPFKANFNIDKPI
                     QSSNLIKLFAWNPGKKAAYLDDFEVTFEY"
     gene            1557..3449
                     /locus_tag="PKI35_08685"
     CDS             1557..3449
                     /locus_tag="PKI35_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HNX44496.1"
                     /translation="MAFDNLTAFIQELERSGELIRISEFVSPGLEITEITDRVSKHRG
                     KALLFENTGTEFPVLINAFGSERRICTALGVKQLDEIGERIKMFFDEMTVFRNGLFEK
                     LAVLPALGEISSWLPHKRRGKGACQEVISENPDLSRLPVLTCWPYDGGPFLTLPAVHT
                     VHPESGIRNVGMYRMQVTGKAETGMHWHLHKGSAAHYREYKRLGRKMPVTVTLGGDPV
                     YIYSATAPLPENIDEYLLAGFIRRKKVEMVKCLTNELYVPADVDFVIEGYIDPEEELM
                     LEGPFGDHAGFYSLADYYPRFHVTCITHRKGAVYPATVVGIPPQEDAWIGKATERIFL
                     APLRIAMAPEIIDMVMPPEGIMHNLVIVKIKNEFPGQPVKVMNALWGVGQMMFNKVMI
                     VVDENANLSDYNALAEAIIQNTDPALDVIFNRGPLDVLDHAGSSFAYGGKMGIDATGK
                     MRDRANHGQQEMSMPGYTALLSHPEVEFVGGVFVSEPLPAVVLAVKKDRPGYVRDLHS
                     RLFHEGALNGFRFVAYIDRFAASLSPRDILWIVCNNIDPVRDCFYPTGGKNEFMLPLA
                     VDGTVKSAKFDGIRRPWPNVVIMDDATIGKIDAIWQNLGIGPFLPSPSLKYKALVKND
                     GAVAEG"
     gene            4185..5420
                     /gene="rocD"
                     /locus_tag="PKI35_08690"
     CDS             4185..5420
                     /gene="rocD"
                     /locus_tag="PKI35_08690"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNX44497.1"
                     /translation="MLSEFLKNLTSEEAMKLEDKYGAHNYHPLPVVLAKGLGPFVWDV
                     EGKRYFDFLSAYSAVNQGHCHPKIVSAMVEQAQTLTLTSRAFYSNILGTYEKYVTGYF
                     GYDKVLPMNSGAEADETALKLCRKWAYQVKGIPENEAKIIVCDNNFHGRTITIISMST
                     DPDAYLGYGPYTPGFIKIPYNDIPALEKALKDPNIAGFLVEPIQGEAGVYVPDDGYLA
                     KASALCKAANVLFIADEVQTGIARTGKLLACDHENVRPDILILGKALSGGALPVSAVL
                     ADDPIMLTIKPGEHGSTFGGNPVACRVAITALEVVKEEKLAENADRLGKIFREELSAV
                     KSPMIKQVRGKGLLNAVIIEPHNGKEAWDVCLKMKDMGVLAKPTHGHIIRFAPPLVIN
                     EQQLREAIGIIKEAILSFE"
     gene            complement(5496..7010)
                     /locus_tag="PKI35_08695"
     CDS             complement(5496..7010)
                     /locus_tag="PKI35_08695"
                     /inference="COORDINATES: protein motif:HMM:NF017754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX44498.1"
                     /translation="MISGFAKPYGISASVIRFSIFNLPENTEMDQQSLKNNIYRQFPY
                     SPTPGQERLIELLTDFVLTGKGNFPLFILEGYAGTGKTTVISALVKVLPRAGYTPVLL
                     APTGRAAKVFSGYAERPASTIHKKLYRSAMDKEGRLTFRLMPNTLHKAFFIVDEASMI
                     PDSRPGENSLFNSRNLLEDLMQHVYSGSAGKMLFLGDNAQLPPVGINYSPALNIEFLR
                     SSFGLDITHYMLTDVVRQSLESGILSVATGIRLKIGNFGSFSPLFGQRNVFPDVRDIN
                     GDEMPDALHECYSHFGRENTVVITRSNKRANLFNQGIRQRILSHESDIAAGDHLMVVR
                     NNYFWLPEETHAGFIANGDIIELQRIGKIEELYGFRFAHVTMRMIDYPDDPAYDVILL
                     LDTLNTETPSLSETENRRLYDAVMEDYMDIPSRLKRMAEVRKNQHFNALQVKHANALT
                     CHKTQGGQWDAVFIDKGYLTNDMLNDEYLRWLYTAVTRAAKILYLVNFEEKFFE"
     gene            complement(6955..9318)
                     /locus_tag="PKI35_08700"
     CDS             complement(6955..9318)
                     /locus_tag="PKI35_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNX44499.1"
                     /translation="MRCRINFNILFLAACFALLALASCNTPRKVQKRGGYLLTKNSIR
                     IDNPYLPSDEIEGFIQQKPVKNTFTYFHPGVWIYRQASHGNPNRFKKYIKQTFGTEPV
                     LYDSNLTLRSASLVKQYAADKGFPAAMIRHNEYYKESHVQVIYRIEAGSPYLVSSFST
                     NIPDTAIGSLYREAGNESLIKTGMIFDTYILDDERDRIAAYLKNKGYYSFSKTDINYT
                     ADTASGNHEVKLVLDINKPVVKNAEKGDSLVRINHPKYYFHNIYIHTDIDAIAAAGGK
                     ADTVIYTYPHRKQDTVARQLYFIQAGKPRIRCSTIANAVVLSEGQPFNMEAGNLTYKR
                     LVNLSAIRSATLSYNPVNPEELIKGGKQWLDCNVRISRNPSYIMNIGTEGTNSGGTMG
                     LGVNTLLSDRNIFRGSETFRMKFTGAAEVQGNLPEGENINKFLFFNTYQAGVVTGLDI
                     PRLLVPFRIFDKSRYSLAQTSISAGYNFEKRPDYTRNITTLFLSYQWSKSEKTRFIFT
                     PLELNYVGIEKDSSFQAYLNALSDPYFVSQYSDHLLSMIRFSITHSNQMLQKQKRLYF
                     IRLTAETAGNMLHAYDKASGSPETGNVYYKHLGVRYAQYLRLDIDLRLFREITSKNTI
                     AARLMAGSGIPYGNSDALPFEKGFWLGGANDMRGWKFRSLGPGTYSSDSIRYDRTGDI
                     VLQGSLEYRFPIYSFINGCLFTDAGNIWLRKENPDFKGSGFRLDTFFDQLAIDSGFGL
                     RFDFSFFVFRLDWGLRTKNPALRDQWFNPDDFRLRKAVWNFGIGYPF"
     gene            complement(9859..11460)
                     /locus_tag="PKI35_08705"
     CDS             complement(9859..11460)
                     /locus_tag="PKI35_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44500.1"
                     /translation="MKTFTLALLAMMATLAARPQVIESFDPITMHIMLGGSNDFSYIS
                     TLANPVPSGINTSPYAGMLFRDKDGVPWCGFWSPLSAPIDLTTDKYVHVKVWKPRISP
                     VRFKIESGAAGTLEIESMSPQTLINEWEELVFDFSSKTGLYGQVVFMPDYLDPVGLTD
                     DITIYFDDIFLNNDPTPGSAPSQMIENLEPIAMNAMLGGADDNSFFSMADNPDPGGIN
                     PSQKVGYFLRDKDGLPWGGFWSALPAPVDVTDNKYVHVKVYKPRISPLHFKIEGGAAG
                     NLEIESTQPQENINQWEDMVFDFSSKTGAYPTIAFMPDFLDPVGLTYDIEIYFDDILV
                     NDDPFPNAMRIVEINVDMRNSGIFDEPVYISGDFGGIWGVWNEPGKNLNDQMLDPDGD
                     SVYSITMNLLDGVYAFKFFKGTGWGGGEWPGDPNRIATITCDNILNCMWGIYGFTNRK
                     ETDSPVVISLYPVPFTDYFTINSPAELSSVTISSLAGKKLLSYGHLSQGKIQIGPVSL
                     PSGMYLVTCTLKTGERHTFKAARLK"
     gene            complement(12130..12204)
                     /locus_tag="PKI35_08710"
     tRNA            complement(12130..12204)
                     /locus_tag="PKI35_08710"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12167..12169),aa:Val,seq:cac)
     gene            complement(12283..17151)
                     /locus_tag="PKI35_08715"
     CDS             complement(12283..17151)
                     /locus_tag="PKI35_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44501.1"
                     /translation="MTKLLLPAILACLLNSVCYSQLVSPPPLTDQQKARLAAVPEVKF
                     QPRRGALSMPYAVDNSAKPYFRPLFAQVGLECGQASSIGLGLTYELNTLRVLPGNVPQ
                     NQMATHFTYNFINGGSNAGVSFLESWEVVKRVGNPSVYDYGGIAYGGPSRWMSGYNLY
                     YNAMHNRITDIRVIYINTIEGLNTLKNWIYNHLDADTIGGVANFYSQTMTVTQKLPTG
                     TPFGGQYVITAFGSSPNHAMTILAYNDSIRYDYNGDGKFTNDIDLNGDGIIDLKDWEI
                     GGFKIANTYGSISNWANQGFAWAMYKAFAENPSSGGIWNNAVYISYAKKDLAPKLTMK
                     VTLKHTSRNKLKVVAGVANNLSATEPEATLNLPIMDYQGGDLYMKGGTTEDDKTIEFG
                     LDATPLLSETVSGQPAKFFLMVQENDPSNQATGQIVSFDLIDYTVGAVTIPGGFSNVP
                     LVENGLTTLSITATINCSKPAIQNTFLPEANLYEPYSQQLNVSGGTPPYKWKFAIDYP
                     ESTDTGSFPMVNTHQLSVNNTASGYASQELPFPFPYYGKTYTRVYPHVDGYLMFEDHQ
                     VPWPYIIYEKTFLKNTKCISPYMGKPLVMMTGEGDGIWYQGCQDSVTFRWRLSVYGAT
                     PSSDLNFAVTLYPDGNIRFYYGDMTTSSYMKWYSGLSNGDGLNYHFTSIYDSLSQPVA
                     GSRFLFTPQEFPDGLSLTDNGQLYGTPLHSCDNLPLKFYVEDNNFLYTTRTLNFKTKG
                     VEIAYEVSSGNDSLIEYGETVYLTPVLKNTCNAALHNIVMHLNVADPYVIPIDSIETI
                     GTLNPGQTVSFPDAFSFSVRNTVPNRYILNLDARLVADEETYTRNSQDTAWSAVPVIS
                     HVAVSDGNDNVLMPGETGNLLVTLHNNGRSMATDISAVLSVIDPYLSIQQGNASVDTL
                     NVNGSPVLSFTITAAAACPPGHVGFLNFHLAGDKGITLNDSVYVNIGPIIEDFETACF
                     TRLPWKFSGNKDWVISQNFPYEGDFCSESGVITDNQESSMYVTLNVLSPSDISFYRKV
                     SSEANYDFLYFCIDGQEQGKWSGEQSWEKFTCQVPAGIHSFLWRYRKDYSISNGSDKA
                     WVDYISWPPLANMLLFASAGPDDNACAGQGHQLSGQVINASSLWWSTSGTGTFMNISQ
                     PDAIYTPSANDLAGGQVTLTIHASQNGLPGVTDNMNLNILPAPVVNAGPDGSVCQGEG
                     FPITQAATANQASVLWTTSGDGSFDNPALLNPVYTPGNNDQLNGTVTLTLTASGPSGC
                     SAVSDAILLTVHPAVVTNAGPDQIIPYNTSAQLSGSASGGSGTFTASWEPSDLLADPT
                     VFNPVTALLTNTQAFTLTASDNNTGCSSSDQILVTVTGGPLSVTTSANPSTICPGDTS
                     QLSATGSGGSGTYTYQWTSNPPGYSSALQNPYVTPAVTTVYTVEINDGAASAFGSVTV
                     TTDTGPSAPARPQGPQAVNVLYTSVSSYFTAGSPNATQYAWHFRPADAGVIVPDNADC
                     DVQWNSAFEGIAALSVVASNECGTSGSSDTLFITANSQVGQPDESPLNELHIYPNPGK
                     DIFNVVVPEAGEYFFRIYNLQGKPVKEGNIRPDPGNMIFRLDMSGMPAGIYHLAVSSN
                     RQNLSGKITLVK"
     gene            17390..18532
                     /locus_tag="PKI35_08720"
     CDS             17390..18532
                     /locus_tag="PKI35_08720"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNX44502.1"
                     /translation="MQEKTSSTVYIPQFDIIRFFAAFMIVIYHSYIAWKGWFGLPGVI
                     SAGDYKTFSGVGLHVDQFIRNMPFGVDIFFFISGYLLTYLLVIEKEKFGKVNVPKFYI
                     RRGLRIWPLYFFLVAVAPLLVNWLGEKQPNYWPTIALVNNFETIHTQQWTYPFSHFWS
                     ICIEEHFYLIWPLIVAFVPFKKLPVAILGLIGFSWLYKISTYLFLDSNWYQLYLHTFS
                     RMDVILLGGLLAVFYIKKPFEFKLHWSIVVIISVVLIFLLSFDDMSNYENMLNGVTRK
                     FVYISLLGLLMMDLLFNPDRKKAMYRFKPLLYLGKVSYGIYMYGNILILIVIKKVMYG
                     FGISNIYFYFLLIFTLSLIVPVISYELFEKPFLKLKQRFALVKTRM"
BASE COUNT         4655 a         4346 c         4770 g         4893 t
ORIGIN      
        1 acactgaaac cctgcccctc acccgcaacc tcacccgggc aggcaggcct gaaaccctga
       61 aacactaagt ataggcaagg aacaaggtgc aaatcgctaa tggctgagag aagccgaatg
      121 taacttacag gagctggaat atccgaaatc agacgctcct aaaaccggaa atgaagtggt
      181 attaattttc gcagtatgat agaatcaaaa ctaatagcgg ttattgctgt cctggcaaca
      241 gtttttatcg gactggcagt atatctgttc cttcttgacc gcaagatcgg gaaacttgaa
      301 aaccagttga aggaacggcg ggaaaagcga tgaaaaaggt ccatgtcatc gttattttgg
      361 ccgtgatggt tgcattgggg gttgttattt ccctgtttac aggcagtgaa acatattccg
      421 atttttcggc agccgcttcc ggcgcatcaa aacaatatca gatatcaggc aggcttaacg
      481 ttgataagcc catcctgtat aatcctgatg aggatgcgaa tacgttttcg ttttatatgg
      541 ccgacgaaaa agggagagaa tgtaaagtta tttaccgggg cggcaagccc agggattttg
      601 agaaatcgga gcagattgta attaccggca atatggaaaa cgattcggtt tttctggccg
      661 gcaacctgct gttgaagtgc ccttcaaaat ataacgatga aaagaggccc gcgcgcttta
      721 ataacaagga attcagatca tccggcgaaa cagcagacta accggaatgc aagcccggga
      781 ataagaacgg ggaaggatgt tgcttccttc cccgccggta tatgaattta ttagatttct
      841 cagtattcaa aagtaacctc aaagtcatcc agataggcag ctttcttgcc cgggttccag
      901 gcaaataatt tgatgaggtt cgatgactgg atcggtttgt cgatattgaa attagccttg
      961 aaaggaatcc aggaattggc ttccttcacc gtagatgtta aggaataagc tttccagtca
     1021 atgggactgt tattttcgct gatgtccata ataatgctca gatcgggtga agctacggtt
     1081 gaataaaccc atccttttac tgtgactttt ttaggaatcc tttgatcaat gttatcgatg
     1141 aactcacgaa atgcatagga gtagacgaat ttgtcatcaa caagggtgac atactggccc
     1201 gaatgtgcag gagccccttc cattttcaca acagtctttt cattcgacca ggagggaatg
     1261 acggataatg cattttcaag gtcattggaa ataaccacag tgttctgatc ttttgcctgg
     1321 tccccggcct tattggcctc tttctgattc gacccggaac atccccataa cgaaattcca
     1381 aggattgcaa gagcgagaat ttttttcata agatacagtt ctaaagtgta ttaattaata
     1441 agtttttgac gaaacaaatg tacaataaat acaaaatcat aaacaaaggc catttgtatt
     1501 aatttcgtgg tttcatcatc cggtgtcaat ctcccgatga gtataatcta attacgatgg
     1561 catttgataa cctgacagcc tttattcagg aacttgaaag atcaggcgag cttattcgca
     1621 tcagcgaatt tgtttcaccc gggcttgaaa tcaccgagat caccgaccgc gtcagcaagc
     1681 atcgtggcaa agcgctgctt tttgaaaata caggcacgga atttcccgtt ctcatcaatg
     1741 cctttggcag cgaaaggcgc atttgtacgg ccttaggggt aaagcaactg gatgagatag
     1801 gggagaggat aaagatgttt tttgatgaaa tgactgtttt ccggaatgga ctcttcgaaa
     1861 agctggcagt tttgccggcg ctcggcgaaa tatcatcctg gcttccgcac aagcgccgcg
     1921 gcaagggcgc atgtcaggaa gttatctccg aaaatcccga tctttcgcgc ctgccggttt
     1981 taacatgctg gccatatgac ggcggaccat tccttaccct gccggcagta catacggttc
     2041 acccggaatc cggaatcagg aatgtgggaa tgtaccggat gcaggtgacc ggcaaagccg
     2101 aaaccggaat gcattggcat ttgcacaaag gttctgctgc acattacagg gaatataaac
     2161 ggctcggcag gaaaatgccc gtcacggtca cgctgggtgg cgatccggtc tacatttatt
     2221 ccgctacggc gccgttgccc gaaaatatcg atgaatacct gctggccgga tttattcgcc
     2281 gcaaaaaagt ggagatggtg aaatgcctga ccaatgagct gtatgttcct gccgatgttg
     2341 atttcgtgat cgaaggttat atcgatccgg aagaagaact gatgctggaa ggcccttttg
     2401 gcgatcatgc cggtttttat tctttggccg attattatcc aagattccac gtaacctgca
     2461 taacccatcg gaaaggtgct gtttatccgg caactgtcgt gggaattccc ccgcaggaag
     2521 atgcctggat cggtaaagca accgagcgga tctttctggc tcctctccgg atcgctatgg
     2581 cgcctgaaat tatagatatg gtaatgcctc ccgagggcat tatgcacaat ctcgtcattg
     2641 taaaaattaa gaatgaattt ccgggtcagc cggttaaagt catgaacgcc ctgtggggag
     2701 tcgggcaaat gatgttcaat aaagtgatga ttgtagtgga tgaaaatgcc aacctttcag
     2761 attataatgc acttgctgaa gccatcattc aaaataccga tccggcgttg gatgtaatct
     2821 tcaaccgggg gccgctggat gtccttgacc acgccggcag cagttttgcc tacgggggca
     2881 aaatggggat agatgcgaca gggaaaatgc gcgacagggc aaatcatggc caacaagaga
     2941 tgtccatgcc cggttatact gcactgctca gtcatccgga agttgaattt gttggcggtg
     3001 tgtttgtttc ggaacctctg ccggcggtgg tccttgctgt aaagaaggat cgtcccggat
     3061 atgtcagaga tttgcattca cgtttattcc atgaaggagc cttaaacggc ttccggtttg
     3121 tcgcatatat cgatcgtttt gctgcatccc tgtccccgcg cgacatttta tggattgtat
     3181 gcaacaacat tgatccggtg cgcgactgtt tttatccaac cgggggaaag aacgaattta
     3241 tgcttccgtt agcggttgat ggtacggtga aatcggcaaa atttgacgga atcaggcgcc
     3301 cctggcccaa tgtagttatc atggatgatg caacgatcgg taaaattgat gccatttggc
     3361 aaaatctggg tatcggccct tttctgccat ccccctcgtt gaaatacaaa gcgctcgtga
     3421 aaaatgatgg agccgttgca gaaggataat cagggggaaa tcacctatgg ctgttagctg
     3481 ttagctttta gctattggcc attgtctctt ggccgttgct ccttgccctg atacttcctt
     3541 atctttactg ctccgcgagg agttgtaagc ccgcagggct ttaaacgacg aaacggtctc
     3601 tacgccttac cggaatattt gttaagaacc ccgcgttccg cccctggctc cctttacctc
     3661 ttgccttgtg gccttgtggc cttgtggcct ttagcctttg gctcttggct cttggtcttt
     3721 agcgtttagt gttctgcgtt ttgtgttctg cgttttccat tctccgttct ccgtccagcc
     3781 ccttgaacca tgctccttgc cccatactcc tagcccatcg cccctgccgt cctcccacaa
     3841 gcttgcgcaa ggcaggtaaa cttttacctt tgacttttta ccttttatct ttgccctgcc
     3901 gtcttaccca cacccgctcc aggcaggtaa acttttgact tttggccatt ggccattaac
     3961 tccttgcact ttactccttg ctccttccgt tttccgtcct ccgttcagtc ccttgctccc
     4021 tgcccccacg ctccctgcat tctcccctgc ctgccacccg caggcttgcg caaggcaggt
     4081 aaagttttcc gttcacctga cctttaacct ttaccctttt cttccttgct atgtccggtt
     4141 aaatatgtac tttcgtaacg cctatcaata aaacctaaat catcatgtta agcgagtttt
     4201 taaagaatct tacctccgaa gaagccatga aactggagga taaatatggc gcgcacaact
     4261 atcatccgtt gccggtggtg ctggcaaaag gtctgggacc ctttgtgtgg gatgttgaag
     4321 gaaagcgtta tttcgatttt ttatcggctt attcggctgt taaccaggga cattgtcatc
     4381 caaagatcgt cagcgctatg gtggaacaag cccaaacgct cacacttaca tcaagggctt
     4441 tttacagcaa catcctggga acatatgaaa agtatgttac cggatatttc ggatatgata
     4501 aagtattgcc gatgaattcc ggggccgagg ctgatgaaac cgcgctgaaa ctatgccgca
     4561 aatgggctta tcaggtaaaa ggaattcccg aaaatgaagc aaaaattatt gtttgtgata
     4621 ataatttcca cggccgcacg attacgataa tctccatgtc gaccgacccg gatgcatatt
     4681 tgggctatgg accttatacg ccgggtttca tcaaaatccc gtataacgat atccccgccc
     4741 tcgaaaaagc gctgaaagac ccgaatatcg ccggtttcct tgttgaacct atccagggtg
     4801 aagccggagt atatgttcct gatgacgggt atctggccaa agcctcagcc ctttgcaagg
     4861 ccgctaatgt attgtttatc gctgatgaag tccagacggg tattgcccgt accgggaaac
     4921 tgctggcctg cgaccacgaa aatgtccgtc ccgatatcct gatccttggc aaggcattgt
     4981 cgggaggcgc attgccggta tcggcagttc tggccgacga ccctatcatg cttaccataa
     5041 aaccgggtga acatggttcc acttttggcg ggaacccggt tgcatgccgt gttgccataa
     5101 ctgcactcga agtagtgaaa gaagaaaaac tggccgagaa tgctgatcgt ctcggaaaga
     5161 tattccgtga ggaattaagc gccgtaaaga gccccatgat caaacaggta cgcgggaaag
     5221 ggctgttgaa tgctgtgatt atcgagcctc acaacgggaa agaagcctgg gatgtatgcc
     5281 tgaagatgaa ggatatggga gtactggcaa aacccaccca cggccatatc atccgttttg
     5341 cacctccgct ggtcatcaac gaacagcagt tgcgtgaagc catcggcatt atcaaggaag
     5401 caattctatc gtttgaatag ccataaactg atggaaagaa aacggcagca gcgtatcttg
     5461 ctgccgtttt tatttcttcc aaaggaggaa attcatcatt caaaaaactt ttcttcgaag
     5521 ttaaccaggt acagaatttt tgcagccctg gtcacggctg tatacagcca ccggagatac
     5581 tcgtcgttga gcatatcgtt ggtgaggtag cctttgtcaa taaaaacggc atcccattgt
     5641 cctccctggg ttttatgaca ggtgagcgcg ttggcatgtt ttacctgtaa tgcattgaaa
     5701 tgctggtttt tgcgaacttc ggccatgcgt ttcagccgtg aagggatatc catataatct
     5761 tccatgacgg cgtcataaag cctgcggttt tctgtttctg acagcgaagg cgtctcggtg
     5821 ttgagggtat ccagtaaaag aatcacatca taagccgggt cgtcgggata gtcgatcatg
     5881 cgcatcgtaa catgtgcgaa ccggaagccg taaagttcct caatcttccc gatacgttgc
     5941 agttcaatga tatcgccgtt agcaataaaa ccggcatgcg tttcttccgg cagccagaaa
     6001 tagttattcc tgaccaccat caggtgatcg ccggcggcaa tgtcgctttc gtgcgacaat
     6061 atccgttgcc ggatgccctg gttaaacagg tttgcccgtt tattcgaccg cgtgatgacg
     6121 actgtgtttt cgcgcccgaa atgcgaataa cattcatgaa gcgcatccgg catctcgtcg
     6181 ccattaatat ccctgacgtc cgggaaaaca ttgcgttgac cgaacagcgg gctgaacgaa
     6241 ccgaaattcc ctattttcag gcggataccg gtggcaactg acagaattcc tgattcgagt
     6301 gactggcgca caacatcggt cagcatgtaa tgggttatgt cgaggccgaa agatgaccgc
     6361 agaaattcga tattcagcgc gggactgtaa ttgatgccta ccggcgggag ctgggcattg
     6421 tcgccgagga aaagcatttt accggcgctt cccgaataca cgtgttgcat gaggtcttcg
     6481 agcaggttgc gggaattaaa aaggctgttt tcgccggggc ggctgtcagg gatcatggag
     6541 gcttcatcca caataaagaa tgccttgtgc agggtattgg gcatcaagcg gaaggtaagc
     6601 cggccttcct tgtccatagc cgaacgatag agttttttat ggatcgttga ggcgggacgt
     6661 tcggcatatc cggagaaaac cttggcagcg cgtcccgtag gcgccagcag cacgggggta
     6721 tacccggcac ggggcaatac ttttaccagg gcgctgatca cagtggtctt tccggtgccg
     6781 gcgtaccctt cgaggatgaa taacgggaag tttcctttcc ccgtcaggac aaaatccgtc
     6841 agcaattcaa taagccgttc ctgcccgggt gtgggggagt agggaaattg tctgtaaata
     6901 ttgtttttta acgactgctg atccatttcc gtgttttcag gcaggttgaa gatgctaaaa
     6961 cggataaccg atgccgaaat tccatacggc tttgcgaagc ctgaaatcat ccgggttgaa
     7021 ccactgatcc ctgagggccg ggtttttggt ccggagtccc cagtcaagcc ggaaaacaaa
     7081 gaaggaaaaa tcgaaacgca ggccgaatcc tgaatcgatg gcaagttgat caaagaaagt
     7141 atcaagcctg aatccgctgc ctttaaaatc gggattttct ttacgaagcc atatattgcc
     7201 ggcgtcggtg aacaaacatc cgttgataaa actgtagatc gggaaccggt attcgagact
     7261 gccctgtaac acaatatcac ccgtccggtc gtagcggatt gagtcggaag aataggtgcc
     7321 gggaccgagt gacctgaatt tccagccgcg catgtcgttg gcgccgccca gccagaatcc
     7381 tttttcaaag ggcaatgcat cgctgttccc gtaaggtata cccgaacctg ccatcagtct
     7441 ggctgcaatg gtatttttgc tggttatttc acggaacagt cgcaggtcga tatcgaggcg
     7501 caggtactgg gcatagcgta cgccaagatg cttataataa acattgccgg tttcaggcga
     7561 accggaagct ttatcataag catgaagcat gttgcctgct gtttcggccg tcagacggat
     7621 aaagtacagt cttttttgtt tttgcagcat ctggttgctg tgggtaatgc tgaaacgtat
     7681 catgctcagc aggtggtcgg aatactggct tacaaaatac ggatcggata gtgcattgag
     7741 gtacgcctga aaacttgaat ctttttcgat tccgacataa ttcaattcca atggggtaaa
     7801 tatgaaccgt gttttttccg atttgctcca ctgatacgac aggaagagtg ttgtaatgtt
     7861 gcgggtataa tcggggcgct tttcaaaatt ataaccggcg ctgatcgaag tctgtgccag
     7921 tgaatatctc gatttgtcga aaatgcggaa aggcaccagc aggcggggga tgtccagccc
     7981 ggtaaccacc ccggcctggt atgtgttgaa gaaaagaaat ttattgatgt tttcgccttc
     8041 aggcaggttg ccctgtactt cggcagcgcc cgtaaacttc atcctgaagg tttcggaacc
     8101 cctgaaaata ttgcggtccg agagcagggt atttacgcct aaacccatgg ttcccccgga
     8161 gtttgttccc tcggtgccga tattcataat gtaggaagga ttgcgggaaa tcctgacatt
     8221 acaatcgagc cactgcttgc cgcctttaat aagttcttcg ggatttaccg ggttatagct
     8281 taaagtggca gatcggatag ccgataaatt gaccagacgt ttgtacgtca ggttgccggc
     8341 ttccatgtta aagggttgcc cctccgataa aacaacggca ttggcgatgg tcgaacagcg
     8401 aatcctgggc ttcccggcct ggatgaaata tagttgacgg gcaacggtgt cctgctttct
     8461 gtgaggatag gtataaatga ccgtatcggc ttttccgccg gcagcggcga tggcgtcaat
     8521 atcggtgtga atgtaaatgt tatggaagta atatttcggg tggttaatcc tgaccagcga
     8581 atcgcctttt tcggcgtttt tcacgacagg tttgttaatg tcgagcacaa gttttacttc
     8641 gtggttgcct gatgccgtat cggcggtata gttgatatcc gtctttgaaa aagagtagta
     8701 gcctttgttt ttgaggtatg cagcaatacg gtcacgctca tcgtcaagga tgtaggtgtc
     8761 gaaaatcata ccggttttaa tcagcgattc attgccggct tcgcggtata acgatccgat
     8821 ggcggtatcg gggatatttg tgctgaaaga cgacacaagg tacggggagc cggcctctat
     8881 cctgtatatg acctggacgt ggctttcttt atagtactcg ttatgacgga tcatagcagc
     8941 aggaaaccct ttgtcggcgg catattgctt taccagggat gcggagcgca aggtgaggtt
     9001 gctgtcgtac agaacaggct cggtgccgaa agtctgtttg atgtatttct tgaaacggtt
     9061 cggattcccg tgcgacgcct gtcggtatat ccatacgccg ggatgaaaat aggtaaacgt
     9121 gttttttacc ggtttctgct ggataaagcc ttcaatctca tccgaaggaa ggtagggatt
     9181 gtcgatcctg atgctgtttt ttgtcagaag gtaaccgccc cttttctgaa ctttccgggg
     9241 cgtattgcac gatgccagag ccagtaaggc aaaacaagca gcaaggaata aaatattaaa
     9301 gtttatccgg catctcatga cagcaaatgt atgaaaatgc gggcaaatgt cgggggcgtt
     9361 aaatgccttc cgcgaaagcg caaccaggaa ggtttgtacc ggagctttaa cttacgaatt
     9421 gtttattaaa caggtgcttc gggcgatacc tggtttccgg aatcagggga agacagatta
     9481 cttgtgagta tggagctata tttttctgaa tcactattgt ccggataaaa cggccatgga
     9541 ttgtttaaaa tattgaatat gtgtcgatta agcccctctc cttcaggaga ggggttgggg
     9601 tgaggtaggg cttgattatc aattactttc tggtgtttcc cggacactag cgttcctgaa
     9661 tataataatt atacctgaaa aaaggagaga ctgtctcaaa agtctgaatc cggtttggta
     9721 aatctcgttg cttctttgtt gagccgcttc ccggcgatgt gtctccaacc cgatggctca
     9781 cgaagaactt cccccctcat caatgcagac tcttaaaaca gtctctttat ggctgcgtaa
     9841 acaggtctta tcaccggttt attttaacct ggctgcctta aaggtatgac gctcgcctgt
     9901 ttttagcgta catgttacca ggtacatgcc ggagggcaag gaaaccggcc cgatttgtat
     9961 tttgccctgc gaaaggtgcc catagctgag caatttcttt cctgccagcg aggaaatcgt
    10021 tacagaagaa agttcggcag gggaattaat tgtgaagtaa tcggtgaaag gaaccgggta
    10081 taacgagatg acaacgggag aatctgtttc tttcctgttt gtaaatccat aaatgcccca
    10141 catgcaatta aggatgttgt cgcaggttat ggtcgcaatc cggttcgggt cacctggcca
    10201 ctcgccgccg ccccagcctg ttcctttgaa aaatttaaag gcataaacac cgtcaagcag
    10261 gttcatggtg atggaataaa cggaatcgcc atcggggtca agcatctggt catttaaatt
    10321 tttacctggt tcattccaaa cgccccatat tcccccaaaa tcgcccgaga tgtaaacagg
    10381 ctcatcgaag atcccggaat tcctcatatc aacgtttatt tccactatcc tcatggcgtt
    10441 tggaaaagga tcgtcattca ccagaatgtc gtcaaaatag atttcaatat catacgtcag
    10501 ccccacagga tccaggaagt caggcatgaa ggcaatggta ggataggcgc cggttttcga
    10561 tgaaaaatca aataccatgt cttcccattg attgatattt tcctgcggct gagtggattc
    10621 aatttcaaga tttccggcgg cgccgccttc aattttgaag tgcagcgggc tgatccgggg
    10681 tttatagact ttgacatgaa catatttatt atcggtgaca tcgacaggcg caggcagggc
    10741 cgaccagaat ccgccccatg ggaggccgtc cttgtcgcgg agaaaatagc ccactttctg
    10801 gctcggattt atcccgccgg gatcgggatt gtccgccatg ctgaaaaagc tgttatcatc
    10861 ggcgccgccc agcatggcat tcatggcgat gggttccagg ttttctatca tctgcgacgg
    10921 ggcgctgccg ggggtgggat cgttgttcag gaaaatgtcg tcgaaataga tggtgatatc
    10981 atcggtaagc ccgacagggt ccaggtaatc gggcatgaat acgacctgcc cgtaaagacc
    11041 tgttttggag gagaagtcaa acaccagttc ctcccattcg ttgataagcg tttgcgggct
    11101 catcgattcg atttcgagtg ttccggcggc tcctgattca atcttgaacc tgacaggact
    11161 gatgcggggt ttccagacct tcacatggac gtacttgtcg gtggtcaggt caatgggagc
    11221 cgacagcggg gaccagaacc cgcaccaggg cacaccgtcc ttatcgcgga acagcatccc
    11281 ggcataaggg ctggtgttga tgccggaagg aaccggattt gccagtgtgg aaatataact
    11341 gaagtcattc gatccgccca gcatgatgtg catggtaata gggtcgaagc tttcaatcac
    11401 ctgcggccgg gcagccaagg ttgccatcat ggccagtaac gcaagcgtaa atgttttcat
    11461 gttcctgttt tttaagatta ctgaatgatt ccggtaaaaa attcagaatt ccgatactat
    11521 ggaatagtac gaaacggact gaatgatgtt cgggaagcac tacatgaaag ctgagacgcc
    11581 ttgcagtaag tacaggagga aggaagtttt taagaagcat cggcaaccag aaggtgccgc
    11641 ataccttaaa gatacaaaaa aaatcgggat tttatcttat gccggtgaat attagtgtat
    11701 tattaccgga agtcagctgc gtcaacccaa caaaaaagcc accctaaagg cggcttcggg
    11761 tgggaattgg caggcctgtc ccggtgaaac cggcgactcg aactgccggc cttcccgatg
    11821 gaatcgggac gctctgaatc gcctgagcta aaataaaaaa gccaccataa aggcggcttc
    11881 gggtgggaat tggcaggctt gtcccggtga aaccggcgac ccgaactgcc ggccttcccg
    11941 atggaatcgg gacgctctga atcacctgac cttaagcaaa aaagccacct ttggggtggc
    12001 tttgggtggg aattggcagg cttgtcccgg tgaaaccggc gacccgaact gccggccttc
    12061 ccgatggaat cgggacgctc tgaatcacct gaccttaagc aaaaaagcca cctttggggt
    12121 ggctttgggt gggaattggc aggctcgaac tgccgacctc atgcgtgtga agcatgcgct
    12181 ctgaaccaac tgagctaaac tcccgaaacc ggtgcaaaag taatagaaaa aacttcctgt
    12241 tccctaaccg ttttgataaa aagacatatt gctgttttcg ggttatttta ccagggtgat
    12301 ttttcctgac aggttttgcc ggttgctgct gaccgccagg tgataaatgc cggcgggcat
    12361 cccgctcata tccagcctga agatcatgtt gcccggatcg ggccggatat tgccttcctt
    12421 caccggtttg ccctgcaggt tatagatccg gaaaaagtac tcgccagcct cgggaactac
    12481 aacattgaag atgtcttttc caggattggg atagatatgc aactcattca ggggagactc
    12541 gtccggttgc ccgacctggc tgttagccgt aataaacagc gtatcggatg aaccgcttgt
    12601 cccgcattca tttgaggcga cgacgctcaa cgcggctata ccttcgaaag cggaattcca
    12661 ctgcacatcg caatctgcat tgtcgggaac gataacgccg gcatccgcag gcctgaaatg
    12721 ccaggcgtac tgggttgcat ttggcgagcc tgcagtgaaa taggaagaaa cagacgtata
    12781 taacacgttg acggcctgag gtccctgggg cctggcagga gcggatgggc ctgtatcggt
    12841 ggtaaccgtt acagagccaa atgccgatgc tgcgccgtca ttgatttcaa cggtatagac
    12901 ggtggttacg gcaggcgtaa cataaggatt ttgcagggca gaggaatatc ccgggggatt
    12961 tgaggtccac tgataggtat aggtccccga gccgccgctg ccggttgccg agagctggct
    13021 tgtatcgccc gggcatatgg ttgaaggatt tgcactcgtt gtgacggaaa ggggtccgcc
    13081 ggtcaccgtc accaggatct ggtcggatga tgaacagccg gtgttgttat cggaagcggt
    13141 taaggtaaat gcctgggtat tggtcagcaa ggcggtgacc gggttgaata ccgtcggatc
    13201 ggcaagcaaa tccgaaggtt cccaggatgc tgtaaacgtc cctgagcctc ctgaagctga
    13261 acctgagagt tgtgcggagg tgttataggg gatgatctgg tcaggaccgg cattggtaac
    13321 cacagcagga tggacggtga ggagtatggc gtcgctcacg gcggagcagc ccgaagggcc
    13381 ggaagcagtg agggttaatg tcacggttcc gttcaattgg tcattatttc cgggagtgta
    13441 aacgggattc agtaatgccg ggttatcgaa actgccgtct cccgaagtgg tccataacac
    13501 ggatgcctga ttggcggttg cagcctgcgt tatcggaaat ccctcgccct ggcataccga
    13561 tccgtcaggg ccggcattaa ccaccggggc aggtaaaata ttcaggttca tattgtcggt
    13621 cacgcccgga agcccattct ggctggcatg gatggtgagt gttacctgtc cgccggccag
    13681 atcattggcg cttggcgtat aaatagcatc tggctggctg atattcataa aagtaccggt
    13741 cccgctggtc gaccaccaga gcgatgaggc attgatcacc tggcccgaaa gctggtggcc
    13801 ttgccctgcg caggcattgt catcaggccc tgctgaggca aacagcagca tgtttgccag
    13861 gggcggccat gagatataat ctacccaggc tttatccgat ccgttcgaga tagaatagtc
    13921 cttccggtat ctccacagga aactgtggat cccggccggc acctggcagg tgaacttttc
    13981 ccaggattgt tctcccgacc atttgccctg ttcctgcccg tcgatacaga agtacaggaa
    14041 atcataattg gcttcggatg aaaccttgcg ataaaagctg atatcggagg gcgaaagcac
    14101 attcagcgta acatacatgc tgctctcctg gttatcggtt ataacgcctg attcagaaca
    14161 aaaatcgcct tcataaggaa aattctgact gataacccag tccttattgc ccgaaaattt
    14221 ccagggaagc ctggtgaaac aagcggtttc aaaatcttcg atgatggggc cgatattcac
    14281 atatacactg tcgttcaggg taatgccttt atcacctgcc aaatggaaat tcaggaatcc
    14341 tacatggccg ggcggacagg cagcggcagc ggtgatcgtg aaggaaagga ccggtgagcc
    14401 gttgacattc agcgtatcaa cggatgcgtt tccctgctgt atcgaaagat aaggatcgat
    14461 aacggaaagg acagcggaaa tatccgttgc cattgacctg ccgttattat gcagcgtcac
    14521 aagcaggttc cctgtttcac cgggcatcag cacattgtcg ttgccgtccg ataccgcaac
    14581 atgcgaaatc accggaacgg cagaccatgc cgtatcctgg ctgttacggg tataggtctc
    14641 ttcgtcggcg acaagcctgg catccaggtt caggatatac ctgttgggaa ccgtgttcct
    14701 gaccgaaaaa ctgaaagcgt caggaaagga aacggtttgt cccgggttca gcgtgccgat
    14761 ggtctcaata ctgtctatcg gaataacata aggatcggcg acattgagat gcatgacgat
    14821 attgtgaagt gcggcgttgc aggtattctt cagtaccggt gtaaggtaca ctgtttcacc
    14881 atattctata aggctgtcgt tccccgaact gacctcataa gcgatttcca cgcctttggt
    14941 cttaaaattc agggttcggg tagtatagag aaagttatta tcctcaacgt agaactttag
    15001 cggcaggttg tcacaggaat gcaggggcgt tccgtataac tggccattat cggtaagcga
    15061 caatccgtcg ggaaattcct ggggggtgaa cagaaatctt gaaccggcaa ccggttgcga
    15121 caacgaatca taaatggatg tgaaatgata attcaggcca tcgccgtttg ataagccgga
    15181 ataccatttc atgtatgatg aggttgtcat atcgccgtaa taaaacctga tattgccatc
    15241 gggatataag gttaccgcaa aattgaggtc tgaggaaggg gttgcaccgt aaacggagag
    15301 cctccacctg aaagtaacac tatcctggca tccctggtac cagatgccgt cgccttcgcc
    15361 ggtcatcatt accagcggct tgcccatgta tggagagatg cactttgtat tttttaaaaa
    15421 cgtcttttca taaataatat acggccaggg aacctgatga tcttcaaaca tcagatatcc
    15481 gtccacgtgc gggtaaaccc tggtatatgt ttttccataa tagggaaatg gaaaaggtaa
    15541 ctcctggctt gcatatccgg aagcagtatt gtttactgaa agctgatggg tattgaccat
    15601 cggaaatgaa ccggtgtctg tcgattccgg ataatcgatg gcaaatttcc atttgtaggg
    15661 aggcgtaccg ccgctcacat taagctgttg gctatagggc tcatataagt tggcttccgg
    15721 gaggaaagtg ttttgtatgg ccggtttgct gcaattaatg gttgccgtga tggaaagcgt
    15781 ggtaaggccg ttttcgacaa gcggaacatt ggagaaccct cccggtatgg tgacggcgcc
    15841 gacggtatag tcgatgaggt cgaatgaaac aatctgaccg gttgcctggt ttgaaggatc
    15901 gttttcctgc accatcagaa aaaacttcgc cggctgcccg ctgactgtct cgctgagcag
    15961 cggggtggca tccagcccga attcaatggt cttgtcatct tcggttgtac cccctttcat
    16021 atacaggtct cccccctgat agtccataat cggtaaattc agcgtagctt caggctctgt
    16081 ggccgaaagg ttattggcta ccccggcaac tactttcagt ttgttccggc tggtatgctt
    16141 aagtgtgacc ttcatggtca gtttgggggc aaggtccttt tttgcgtaag aaatatatac
    16201 ggcattattc cagattcccc cgctggacgg attctcggca aaggctttat acatagccca
    16261 ggcaaatccc tggtttgccc agtttgatat acttccgtaa gtattggcta ttttaaagcc
    16321 cccgatttcc cagtctttca ggtcaatgat cccgtcaccg ttcagatcaa tgtcgttagt
    16381 gaatttcccg tcgccgttat aatcgtaacg gatggagtcg ttataggcga gaatggtcat
    16441 ggcatggtta ggactgctgc cgaatgcggt aatgacatac tgcccgccaa aaggggtgcc
    16501 ggtcggaagt ttttgggtga ccgtcatggt ttgcgagtaa aaattggcaa ccccgccgat
    16561 agtatcggca tccagatggt tatagatcca gtttttcagc gtattaagcc cttcgatcgt
    16621 attgatataa atgaccctta tatcggtaat ccggttgtgc attgcgttgt aataaagatt
    16681 atatcccgac atccaccttg acggaccgcc gtaagcaata ccgccgtaat cataaacgct
    16741 cggattaccg acccgtttta caacttccca cgattcaagg aaactgacac cggcgttgct
    16801 tcctccgttt ataaagttgt aggtgaaatg ggtggccatt tgattttgcg gcacattacc
    16861 gggcaggacg cgcaatgtgt taagctcata ggtcagcccg agcccgatgc tgctggcctg
    16921 tccgcattcc agtcccacct gggcgaagag aggcctgaaa tagggtttcg ccgaattatc
    16981 gacggcgtaa ggcatggaga gcgctccgcg acgcggctgg aatttgactt ccggaaccgc
    17041 ggcgagtctg gctttttgct gatcggtcaa gggcggaggt gaaaccagtt gtgaataaca
    17101 gactgaattc agtaaacagg ctaagatagc agggagtagt aattttgtca ttgtgaagca
    17161 taaaaactca ttaaaagtaa aaatttattc gtttcacccc ggatgaattt catcagaggc
    17221 aaccgtcagt tttttattac ggcagcccag gctcccggcg ccggtggctt ccggttgcca
    17281 gtcgcggttt tcccggtttt tagccggctg ttgcctgaaa aaagtaccgc gaattttatc
    17341 cggtttctaa atctccttat ttttgaaaat tattcaaaac ctgatctcct tgcaagaaaa
    17401 aacctcatcg acggtatata tccctcaatt tgatattatc cgtttcttcg ccgccttcat
    17461 gattgttatt tatcattcct atattgcctg gaaaggatgg ttcgggttgc cgggcgtaat
    17521 ttcggccggc gattacaaaa ccttttccgg cgttggcctg catgttgacc agttcatcag
    17581 gaatatgccg ttcggcgtcg atatcttttt ctttatcagc ggttacctgt taacctatct
    17641 gttggtcata gaaaaggaaa aattcggcaa ggtaaatgtc ccgaagtttt atatccggcg
    17701 tggtttgcgt atatggccgc tttacttttt tctggtagcc gtcgctccgc tgctggtaaa
    17761 ctggctcggc gaaaagcaac ccaactactg gcctaccatt gcattggtca ataatttcga
    17821 aaccatccac actcagcaat ggacgtatcc tttctcccat ttctggtcta tctgcatcga
    17881 agagcatttt tacctgatct ggccgttaat tgtcgcgttt gtccctttta aaaaattacc
    17941 cgtggccatc ctggggctta tcggttttag ctggctctat aaaatcagta cctatctttt
    18001 cctggactcg aactggtacc agttgtacct gcataccttc agccggatgg atgttattct
    18061 gctgggcggg ctgctggccg ttttctatat aaaaaagcct tttgaattca aacttcactg
    18121 gagtatcgtg gtgataattt cagtggtgct tatcttcctt ctttcattcg atgatatgag
    18181 caactatgaa aatatgctga acggggttac ccggaagttc gtttatatct ccctgctagg
    18241 cctgctcatg atggacctgc tgtttaaccc ggaccggaaa aaggccatgt accggttcaa
    18301 acccttgctg tacctgggga aggtttccta tggcatctac atgtacggta atatcctgat
    18361 tcttatcgtc atcaagaaag taatgtacgg attcgggatc agcaatatct atttctattt
    18421 cctgctgatt ttcactttat cgctcatagt tcccgttatc agttatgaat tgtttgagaa
    18481 gcctttcctt aaactgaaac agagatttgc gctcgtgaaa acgaggatgt aaaaggaagt
    18541 tggaactcac ggaaatcctg gtttacatgc agggctgagg tggataatca aaaaaaatcc
    18601 cggaagaatt tcgagggcac tgaaaaagtc catcggacat aattgttaaa ataaaagcag
    18661 taaa
//
LOCUS       DAOPYV010000062        18298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_3191_length_18298_cov_13.128817, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000062 DAOPYV010000000
VERSION     DAOPYV010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18298
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3191_length_18298_cov_13.128817"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..1658
                     /locus_tag="PKI35_08725"
     CDS             267..1658
                     /locus_tag="PKI35_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HNX44503.1"
                     /translation="MKNKYIDLIEQTFDFPTDEFEVIDNELYFQNIPLMEVIKQYGTP
                     LRITYLPKIGKQIQKAKRLFNVAIAKADYNGEYHYCYCTKSSHFSFVMEEVLKNDVHI
                     ETSSAFDLPLIESLYEAGKFKKDNYIICNGFKRPQYIENICNLIEEGFENVIPILDNK
                     FELDEYDRNITKKIKIGIRIAAEEEPKFDFYTSRLGIRYNDIIPYYEQEIKNNPKFEL
                     KMLHFFINTGIRDSAYYWNELRKCVDVYCELKAICPQLTALNIGGGFPIKNSLSFDYD
                     YEYMAEEIIAQIKSHCERTSTPEPDIFTEFGSFTVGESGAILYSIIDQKRQNDREVWN
                     MIDSSFITTLPDTWAIDQKFILLSVNHWDKEYERVFLGGLTCDSEDYYNTETHTNAIF
                     LPKLMDDEPLYIGLFHTGAYQESLAGYGGIQHCLIPAPKHILIDVDEDGEYTTKLFAK
                     EQSHKSMLKILGY"
     gene            1810..2664
                     /gene="speB"
                     /locus_tag="PKI35_08730"
     CDS             1810..2664
                     /gene="speB"
                     /locus_tag="PKI35_08730"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNX44504.1"
                     /translation="MKQYGDLPKEYTSFDSARVVILPVPYDGSSTWLKGADKGPAALL
                     EASANMEVYDIETDSEVHKIGIHTADPIEVDGSPEDMADAVYKEMAHLLKKKKFPVII
                     GGEHSVSIGAFKAVAEQFNDLTILQLDAHADMRDSYEGSKCSHACVMARAKEAAPVIQ
                     LGIRSMSVEEREDIQPDRIFYASAIVQNKAWYYDLLNKLTRNVYITIDLDVFDPSIMP
                     STGTPEPGGMSWYEVLDILKKVSEMTNIVGFDVVELKPEKENKSPDFLASKLIYTLLT
                     YKFITGIK"
     gene            3025..4311
                     /locus_tag="PKI35_08735"
     CDS             3025..4311
                     /locus_tag="PKI35_08735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX44505.1"
                     /translation="MKIKKIKPGYLFSTGYTNYIFVLLFLLYMFDYLDRTVVSSMFTY
                     MEKDWGLTHTQCGLLVSSVYWAIVILTFPISILVDRWSRRKTIGVMAVLWSLATALCA
                     FTGNFMQLLLARMLIGVGEAGYAPGGTAMIAGLYPQEKRSQMIGIWNASIPLGTALGM
                     ALGGLIASTLGWKHAFGLVAIPGFIVAILFFFLKDYKTVGLNLPKSGKQEEKTTRSAI
                     INEFLQKPSFIYTSFGMAAVIFVTSTLMTWLPRYFQETGDLDARQAGFRGSIVLFLAI
                     VGGPIGGFLTDRWRKHNIRARLLFPTLTTSLSAILLFIALNFTSGISQFIVLLVFGIS
                     VTAFASAVIAVTQDVIHAGLRAVSYSIAVVVQNLLGASTAPVIIGALYDKWGIQEAML
                     VLPFVLMAGAILFYLGSRYYESDFNKVIKVDLEAED"
     gene            complement(4321..5103)
                     /locus_tag="PKI35_08740"
     CDS             complement(4321..5103)
                     /locus_tag="PKI35_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX44506.1"
                     /translation="MVNDPLSILCCPVCKNSLCTGNNNPGEKETGRCCYCVQCNRNYF
                     SGGGIIRFINDDEVRKFSRRFNFFRAVYARIYTPLTRLLFIPCGGENKARHEVLDKLE
                     LSPGGTILETGIGTGDNLPFIQPFFGSGIYVGLDNQQRMLNACRKKLKRLEMDAELHL
                     ANAEQLPYKDEVFDVVFHLGAFNLFSNKKQALEEMIRVAKPGTRIVIADETEKACQLF
                     SILLGKQEPVILPDYLVPDSMLEKKLEIIWNGYGYLLSFRKP"
     gene            complement(5209..6003)
                     /locus_tag="PKI35_08745"
     CDS             complement(5209..6003)
                     /locus_tag="PKI35_08745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX44507.1"
                     /translation="MTAIKTKTALVTGAASGFGFEFAKLLASDRYALILADKDEARLA
                     MVKEQLQDQFNVPAGTIVSDLSKPQAASEIYAETKDMAIEILINNAGYGLFGFFTQTD
                     WEKEEKMIGLHVTTTTHLTKLFVSDMVKRGNGKIMNVSSMAAFQPGPLMAVYYATKTY
                     LLSFTEALANEVKGTGVTVTAFCPGQTKTGFQDNVARASNAHRSKSAWIADPAKVARV
                     GYHAMQSGKPLSVPGVGNKSIVLLNRFLPRRVVISLVRILQENIRK"
     gene            complement(6178..7152)
                     /locus_tag="PKI35_08750"
     CDS             complement(6178..7152)
                     /locus_tag="PKI35_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNX44508.1"
                     /translation="MEKGPISQFIQHHYRHFNSATLIDAAKAYEEHLLEGGKMMITMG
                     GAMSTAELGISLAEMIRQDKVQIITCTGANLEEDIMNLVAHSHYKRVPGYRDLTPEQE
                     FELLQNGMNRVTDTCIPEEEAFRKIQHHIEKIWKDAQAKGERYFPHEYMYKLLLSRVM
                     EKDYEIDPKNSWMLAAAEKNLPIIVPGWEDSTMGNIFASYCIKGELVPSTVRTGIEYM
                     MYLTDWYRKNSGGKGVGFFQIAGGISGDFPICVVPMMYQDLEWHDVPFWSYFCQISDS
                     TTSYGSYSGAVPNEKITWGKLDIHTPKFIIESDATIVAPLVFAWILGW"
     gene            7389..9185
                     /gene="typA"
                     /locus_tag="PKI35_08755"
     CDS             7389..9185
                     /gene="typA"
                     /locus_tag="PKI35_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNX44509.1"
                     /translation="MQSIRNIAIIAHVDHGKTTLVDKILYACKLFRENQETGELILDS
                     NDLERERGITITSKNVAVEYKGVKINIIDTPGHADFGGEVERVLKMADGVLLLVDAFE
                     GPMPQTRFVTQKALSLGLKPIVVINKVDKENCRPDEVQEQVFELFFNLEANDDQLDFP
                     TIYGSGKNGWMSEDWQVQTTDITALLDAILKYIPPAPQPQGTLQMQITSLDYNSYVGR
                     IAIGRVSRGEIHDTEAVSLVKKDGAIVKSRIKELYKFEGLGRVKTDVVYAGDIGAVVG
                     LEGFEIGDTIADIENPEALPGIKIDEPTMSMLFTINNSPFFGREGKYVTSRHISDRLQ
                     KETEKNLALRVEPGDTPDQFLVYGRGILHLSILIETMRREGYEMQVGQPQVIIKEIDG
                     VKNEPVESLIVDTPEAVAGRVIEIVTQRKGELLVMEPKGDLQHLEFHIPSRGIIGLRN
                     AVLTATAGEAILAHRFIGFEPWKGQLPQRLAGVMVSIETGMATAYSIDKLLDRGRFFV
                     EPFEEIYEGQIVGEHIRDNDLGVNIVKGKQLTNFRTTAKDDAVRLPPAVKFSLEEALE
                     YIQVDEMVEVTPKSMRMRKILLKELDRKRAAK"
     gene            9248..11401
                     /locus_tag="PKI35_08760"
     CDS             9248..11401
                     /locus_tag="PKI35_08760"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNX44510.1"
                     /translation="MKLRLIALLLISCLFFSFNAFSEEGMWLPALISKYNYADMQKMG
                     LKLTPDQIYSINQACLKDAVVALDHGGCTGSVISKKGLLITNHHCGFDAIADQSSVDH
                     DYLTDGFWAMRQEEELPLPGKTVSFLIRMEDVTQAVLAGIPDMLDEESRSEKIEKAID
                     SISNAVKGETKYEVSIESMLAGNEYYLFVYQTYSDIRMVGAPPSGIGKFGGETDNWMW
                     PRHTGDFCLLRVYTGTDGNPADYSKQNIPLSPKEFLPVSLAGVHEGDFSMILGYPGAT
                     DRFLTSYGIRQKLDQLNPSDILLKTAKMDIIRHYMEQSDALRIAYASEYAYLANFQKK
                     SIQESKALKRLNVFDDKKKQEDDFASWVNADVARQVRYGTVLDSLKAIYDQKMNDRAD
                     EAQNYLIESVSGIQLLNFAYQANELASYLADNDSVAEAVADYRNRAKEFYKNYDLQVD
                     KEIMQKMFTLYRDNLPEEYFPEFFEMVKKKFGNDFNRYADYVYSKSVFATPETFEAFL
                     NKPSLKKLEKDPAFTAANNLVASFILTRFIQMSYEEQFNRLRRLYIAGMREMNPGKAF
                     YPDANSTMRLTYGQVKSYVPADAVEYNFQTTVSGVIQKDDPSNAEFRVPQKLKELYAR
                     KDFGRYAENGVLPVCFLTTHDITGGNSGSPVINANGQLIGVAFDGNSEAMSSDIKYDV
                     NLQRTIAVDIRYVLFIIDKFAGAGYLVDEMELAGK"
     gene            complement(11571..12188)
                     /locus_tag="PKI35_08765"
     CDS             complement(11571..12188)
                     /locus_tag="PKI35_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44511.1"
                     /translation="MQDSTNVLIEKIREQLANYKPVQLTLQALSADLGLPLSELTETF
                     RNEAGLVEEILKFENHNLEAIFTDNTFEEDNAIDGLLEVSKVLSDRFRKILPVFSFDL
                     KEYFPELQQKYFTLRFNFVFDKISRNIRLGINQGLYRPDLSKELVSRIYISRLIDLHN
                     PDFFPPDQFSFNTLFNVMFDTFIRGIVTEEGVNYYENKVKSYTFQ"
     gene            complement(12305..13885)
                     /locus_tag="PKI35_08770"
     CDS             complement(12305..13885)
                     /locus_tag="PKI35_08770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44512.1"
                     /translation="MTRSSIHPIIRCNPVLFLLLLIPELMVAQYRNIVIDSTSQPNET
                     VIFVNPENTDYLLAGANLQSIYHSMDGGESWNRVAMSSQFNVWGDPCVVIDTLGYYYY
                     FHLSWPSGGSFIDRIVCQRSIDQGQTWNTGTGIGLNGTKAQDKEWACVNPFNNEIYLT
                     WTQFDVYGTGNPNDSTIIRFSKSSDQGITWSEPVRISEKAGDCLDDDNTVEGAVPCVG
                     PEGQIYVSWAGPTGIVFDRSTDGGNTWLSHDIFVSDFPGGWNYDIPGISRCNGLPVTC
                     CDMSNGIHRGAIYINWTDQRNGADDTDVWIVKSTDGGNTWSAPVRVNDDASGRHQFFT
                     WMTVDQSNGYLWFIFYDRRNYNDSRTDVYLAVSKDGGETFRNFKISQTPFVPNSGIFF
                     GDYNGISAHNNKVHAMWTRLSQQKLSVLTALVDTIYTGLPPVDHAPFSVESTYPNPFA
                     GSAYFSFKVREPAYVTLEVYDIMGQKVATIIGNERMIPGKYTRQFNPAGLNLSSGVYY
                     FRLSSAGKFTYRKIVFQK"
     gene            14092..15111
                     /locus_tag="PKI35_08775"
     CDS             14092..15111
                     /locus_tag="PKI35_08775"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNX44513.1"
                     /translation="MKIGILTAGGDCPGINAAIRGVGKTAITEYGMRVFGISSGFRGL
                     IEKEYTEFDESQLSGILTLGGTILGTSRDKPFKNDPNVENKIPKIIRNNYQEMGLDAL
                     VVIGGNGSMKTAQKLYEEGLNVIGLPKTIDNDVWGTDVSFGFDSAVWIATEAIDRLHT
                     TANSHKRTMVIELMGHHAGWISLYSGVAGGGDVILIPEIKYNIDIVTEYLIKRHQNKK
                     PYSIVLVAEGIEKPKGMHAGTFIAQAIQDKTGFETRDTILGYIQRGGSPSPMDRILAT
                     RYGAHAVELIADGKFGVMVNLTGDQVTSVPLTEVYNKTKLVPRDHSLIDKARKLGICF
                     GDKRV"
     gene            15171..16952
                     /locus_tag="PKI35_08780"
     CDS             15171..16952
                     /locus_tag="PKI35_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNX44514.1"
                     /translation="MKLTLLFIALSLTGTIVLSAQNPELQTDFEKSEGLRTPRYEETV
                     NYCKKLDAASPLITYATFGVSPQGRDLPLLIADSKGRTDVKAIRKSGNIVLLIQACIH
                     AGEPDGKDAGLMLLRDMVTQPSCRALLDHVSVLFIPIFNVDGHERFGPYNRINQNGPE
                     EMGWRTTAQNLNLNRDYLKADAPEMKDWLALYNYWMPDFLVDCHVTDGADYQYVLTYA
                     LETHGTMEQHLSDWVAGTVEPYLVNEMDKAGYPVFPYVEFREWHDPRSGLVVGAAPPM
                     LSQGYCAIQNRPALLIESHMLKPYKQRVEGTYTMLQLLIGKLNHEAGNLTKLVREADE
                     YTCGKQFRSGEFPVNFGESLSDSVMVDFRGMEYTADTSDLTGGLWFRYDNSKPVIWKI
                     PMFPHPRPDVSVKLPEAYIIPPEWCNIICTLKLHGVEMHEILQEQTIEVSTYRFSNAR
                     WQQRPYEGRHKVQCAVNETVEKRTFPAGSVVVPMNQRTARVIAHLLEPRSPDSFVSWG
                     FFDVVFEQKEYFETYVMEPEARKMMLENPALKKEFEKLKASDKGFAADQYAQLMWFYN
                     KTPWSDKQFNLYPVGRIMDGEIVKKLK"
     gene            complement(17160..>18298)
                     /locus_tag="PKI35_08785"
     CDS             complement(17160..>18298)
                     /locus_tag="PKI35_08785"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX44515.1"
                     /translation="MIKRKLEPVITNRLFKGKAIIVIGARQTGKTTLLREITSGFRSE
                     TLWLDADDPVVKAQLTDANISALKLLTAGYKIVVIDEAQQVKNIGKTLKLITDHISDV
                     QLMVSGSSALELAEEISEPLTGRKFEYYLYPLSYEELSNHFGRLDELRMLEQRLIYGS
                     YPDVVNSASTVKETLGLLAGSYLYKDIFKYQDLRKPDLLEQLLRALALQIGSQVSYHE
                     LGRLIGANIETVQRYIELLEKTYVIFRLRSFSRNFRNELKKSRKIYFYDNGIRNALIN
                     NYSPLDLRTDKGALWENYLVSERIKYLHNNGLMANRYFWRTQQQQEVDYIEDIDGVLH
                     AYEFNWNSDAGSKLPLTFSNIYPNNVFKLVTSGNFDSFLKTEEP"
BASE COUNT         4876 a         4198 c         4397 g         4827 t
ORIGIN      
        1 atatcctcct attcattaag atagaatatt gtggtaatat gcggaattga gtatattaaa
       61 tggatttaat acagataatt tagtgttttt cagcttctgg tcttataaag ttaatggttt
      121 tatgacctat ttcggttctg aaaaacaatc cggcagtcgc ggatggctaa aaaaatgata
      181 aagtaattca ctccgattac tttttttatg tcgcaggcct gctcattgat tatctttgtt
      241 ccataaattt catcctgtcc ggctaaatga aaaacaagta catcgacctg attgaacaaa
      301 ccttcgattt ccctaccgat gaattcgaag taatagataa tgagctgtat tttcagaaca
      361 ttcctttgat ggaggtgatc aagcaatatg gcacgccgct gaggattacc tacttaccga
      421 aaatcggaaa acagatccag aaggccaaga gattgtttaa tgtggccatt gcaaaagccg
      481 attataatgg cgaataccat tattgttact gtaccaagag ctcgcatttt tcattcgtaa
      541 tggaagaagt gcttaaaaac gatgtgcaca tcgaaacttc atcggccttc gatcttccgc
      601 tcatcgagag tttatatgaa gcaggtaaat tcaaaaagga taattacatt atctgcaacg
      661 gattcaagag accccagtat atcgagaaca tctgcaacct gattgaagag ggatttgaaa
      721 acgttattcc gatccttgat aataagtttg agcttgatga atatgaccgg aacatcacga
      781 aaaagattaa aatcgggatc cgtatcgctg cagaagaaga acccaagttt gatttttata
      841 cctcgcggct cgggatccgg tacaacgata tcattcccta ttatgaacag gaaataaaga
      901 acaatccgaa gttcgagctt aaaatgctgc atttcttcat caatacggga atccgcgatt
      961 cggcctatta ctggaacgaa ttgcgcaaat gtgtggatgt ttactgcgaa ttgaaagcga
     1021 tttgtcccca gttgacggcg ctcaatatcg gcggtggttt cccgattaaa aattccctga
     1081 gctttgatta tgattacgag tatatggctg aggagatcat cgcccagatc aagagccatt
     1141 gtgaacgcac cagcacgccg gaacccgata tttttacgga attcggctcg tttaccgtag
     1201 gcgaaagcgg cgccatcctt tattccatca ttgaccagaa gcggcagaac gaccgggaag
     1261 tctggaacat gatcgacagc tcttttatta ctaccttacc cgatacctgg gctattgacc
     1321 agaaatttat cctgctatcg gtaaatcact gggataagga atatgagcgg gttttcctcg
     1381 gcggactgac ctgcgacagc gaagattatt acaatacgga gactcatacg aacgccatat
     1441 tcctgccgaa gctcatggac gatgaaccct tatatatcgg tttatttcac accggcgcct
     1501 accaggaatc gctggccggt tacggaggaa ttcagcattg cctcattcct gccccgaaac
     1561 atattcttat cgatgttgac gaggacggcg aatacaccac caaacttttt gccaaggaac
     1621 aaagtcataa atcgatgctg aagatactcg gttattaggc aatctttctt tcggggaaca
     1681 gtaagacaat caacagttta ttgaccgtat tcatttgccc gctaaggaca aagggttgaa
     1741 tatttccgga aatatgtatt ttagcctgtt ctagacagca gatttgccaa tttaaatttg
     1801 ttcaaagcca tgaagcaata cggtgattta ccaaaagaat acacatcgtt cgacagcgcc
     1861 agggtggtta tccttcctgt tccttacgac ggatcgagta cctggctgaa gggcgcggat
     1921 aaaggcccgg cggcgctcct ggaagcatcg gcaaatatgg aagtttacga tattgaaacc
     1981 gacagcgaag ttcataagat aggtattcat accgccgacc cgatagaggt ggacggcagt
     2041 ccggaagata tggcggatgc tgtttataag gagatggcgc acctgctgaa aaagaagaaa
     2101 ttccccgtaa ttatcggggg tgagcattcg gtgagcatcg gagcatttaa agccgttgct
     2161 gaacaattca acgacctgac gattttacag ttggatgcgc atgccgatat gcgtgattca
     2221 tacgaaggtt cgaaatgcag ccatgcctgc gtgatggcgc gggcaaaaga agcagcgccc
     2281 gttattcagc ttgggatcag gagcatgagt gtggaggaac gcgaagatat ccagcccgac
     2341 cgtatcttct atgcctcagc aattgtccag aataaggcat ggtattacga ccttttgaat
     2401 aagctcaccc gtaatgttta catcaccatc gacctcgatg ttttcgatcc ttcgatcatg
     2461 ccgagcacgg gaactcccga accggggggc atgtcgtggt atgaggtgct cgatatcctg
     2521 aaaaaagtga gtgagatgac aaacatcgtt ggtttcgacg tagtggaact caaaccggaa
     2581 aaagagaaca aatcgccgga tttcctggca tcaaaactta tttacacttt actcacctac
     2641 aagttcataa ccggcatcaa atagaaattt ctccggaatg gattgtacgg ggctgctgcc
     2701 aagggaagcg accgtcttca acataccagg ctatagtggc cataaataac gagtgcaatg
     2761 tcaatggcgt ttccgcgggg agttggcgag agaccctgaa ggtttttaaa accttcaggg
     2821 tctgattagc cggcaggcct tctcatggag agacaggctg cacttctccc ctggaaagat
     2881 tgataagttt ttatctgagt aaaccgccta atgaaaagta tatcctatat ttgaggtgtt
     2941 taaatcttat tttcctccgg aaacaataat tctggattgt agtttccggc cggcggtgac
     3001 tgacattgct tttatcggat actcatgaaa atcaagaaaa tcaaacctgg ttacctcttc
     3061 tcaaccggtt ataccaacta catctttgtt ctgctgttcc tgttatatat gttcgattac
     3121 ctcgacagga cggtcgtttc ttcgatgttt acatacatgg aaaaggactg gggactgacg
     3181 cacacccagt gcggtttgtt ggtttcgtcc gtttactggg caattgttat tcttactttc
     3241 cccatttcaa ttcttgttga ccggtggagc cggcgcaaaa cgatcggtgt gatggctgtt
     3301 ttgtggagtc tggctactgc attatgcgcc tttaccggga atttcatgca attgttgctg
     3361 gcaaggatgc tgatcggtgt cggtgaagca ggatatgccc cgggcgggac tgccatgatc
     3421 gccggattgt acccgcagga aaagagatcg cagatgatcg ggatatggaa tgcttcaatt
     3481 cctctcggaa cagcccttgg catggcactc ggcgggctca ttgcttccac ccttggatgg
     3541 aaacatgctt tcggcctggt agctattccg ggttttattg tagccatcct gtttttcttt
     3601 ttgaaggatt acaagacagt ggggcttaat ttgcccaaat ccggcaagca ggaagaaaaa
     3661 acaacccgtt cggccattat caatgaattc ctgcaaaagc catcttttat ctatacttcc
     3721 ttcgggatgg cagccgtgat ctttgtaacc tccacgctga tgacctggct tccaagatat
     3781 tttcaggaaa ccggagacct ggatgccaga caggcaggct tcaggggaag cattgtactt
     3841 tttctggcca ttgttggcgg acctatcgga ggattcctta ccgaccgctg gaggaagcac
     3901 aacatcaggg ccaggttact tttcccgacg ctaaccactt ctttatcggc gattctcctg
     3961 tttatagcgc ttaattttac ttcaggcatc tctcaattca ttgttctgct ggtttttgga
     4021 atatcggtta ccgcctttgc ttcggctgtg atcgccgtaa cccaggatgt gattcatgcc
     4081 gggctcaggg ctgtatcgta ttccattgcc gttgttgtcc agaatctgct tggcgcctca
     4141 acagcaccgg ttatcatcgg cgccctttat gacaaatggg gcattcagga agccatgctg
     4201 gtccttcctt ttgtcctgat ggcaggggcg atcctttttt acctgggatc gcgttactat
     4261 gaatcggatt ttaacaaggt tattaaagtt gaccttgaag ctgaggattg atggcctggt
     4321 ttatggtttt ctgaatgaaa gcaaatagcc gtaaccgttc catattattt cgagtttttt
     4381 ctcgagcatt gaatccggaa caagataatc cggcaggata acaggctcct gtttgcccag
     4441 caaaatgctg aataattgac aggctttctc cgtttcgtcg gctatcacga tgcgggtgcc
     4501 tggtttggct acccttatca tctcctcaag ggcctgtttc ttattgctga aaaggttgaa
     4561 tgcgcccagg tgaaaaacca catcgaatac ttcatcctta taaggtaact gttcagcgtt
     4621 ggcaagatgc aattccgcat ccatttccaa tcgcttcagc ttctttctgc aggcattcag
     4681 catgcgttgc tgattatcaa gccccacata aataccagaa ccgaaaaacg gctgaataaa
     4741 cggaagatta tcgccggttc cgataccggt ttcaagaatt gttcctcccg gtgatagctc
     4801 aagcttatca agcacttcat gccgtgcctt gttttcacct ccgcacggaa taaacagcag
     4861 acgtgttaaa ggcgtataaa tcctggcata aacagcccgg aagaaattaa acctcctgct
     4921 gaattttctg acttcatcat cgttaatgaa ccggatgatc cctcctccgg aaaaatagtt
     4981 tcggttgcat tgcacacaat agcagcaacg gcctgtttct ttctcgccgg gattattatt
     5041 tccggtacag aggctgtttt tgcataccgg acaacataaa attgataaag gatcattaac
     5101 catgactgat tacaatgatc attggtatct gagtcagaaa cagtgaagaa aagtcactca
     5161 gcattgagag caggaaggaa gatcgttatc aggagatgaa ggcggggatt atttcctgat
     5221 attttcctga agtatacgga ccagggaaat gacaaccctt cgcgggagga aacggttgag
     5281 caggacaatt gatttatttc ccactccggg aacagaaaga ggttttccgg attgcatggc
     5341 atgatagcca acgcgtgcca cttttgccgg atcggctatc caggcggatt tactccggtg
     5401 agcgttggat gccctggcta cattatcctg aaaccccgtt ttggtttgcc ccgggcaaaa
     5461 tgcggtgacg gtaacacccg tgccttttac ctcgttggcg agggcttcgg taaacgataa
     5521 aaggtacgtt ttggtggcat aatacacagc catcagggga cccggctgaa atgccgccat
     5581 ggaagaaacg ttcatgattt tgccgttacc ccgtttaacc atgtcggaga caaaaagctt
     5641 ggtgagatgc gtggtcgtcg taacatggag accgatcatt ttttcttcct tttcccaatc
     5701 cgtttgggtg aaaaaaccga acaatccata accagcatta ttgatcagaa tttctattgc
     5761 catgtctttt gtttccgcat aaatttctga tgcagcctgc ggtttcgaaa gatcggagac
     5821 gatggttcct gcaggaacat tgaattggtc ctgcaactgt tcctttacca tggccagccg
     5881 ggcttcgtcc ttgtcggcca gtatcagcgc atacctgtcg gaagccagca gtttagcaaa
     5941 ttcaaatcca aaccccgacg cagctccggt gaccaaagcg gtttttgttt ttatagctgt
     6001 catgattttc cgcaaaagaa tacaggcgcc tgcaaggtat gaagaaaact tcagacagag
     6061 accttctgtg aggccgggtt catcggaata acgcagcccc cccgtcactt tccattgcaa
     6121 tggcatctca gcaaccgatg gttgcgggat tgtactttac gagtcagccg tcagccttta
     6181 ccaacccagt atccatgcga acaccaacgg ggccacgatg gtagcatcgc tttcgatgat
     6241 gaatttcgga gtgtggatgt cgagtttgcc ccaggttatt ttctcattgg ggacagcgcc
     6301 ggaataggaa ccgtaagagg ttgttgaatc ggagatctgg cagaaatagc tccagaaagg
     6361 aacatcgtgc cattctagat cctggtacat catcggaacc acacagatgg ggaagtcgcc
     6421 tgaaataccg ccggcaatct ggaagaagcc gacgcctttg cctcccgaat tcttgcggta
     6481 ccagtcggtg agatacatca tatattcaat gccggtcctc acggtggaag gcaccagttc
     6541 gcccttgatg caataggaag cgaaaatatt gcccatggtg ctgtcttccc agccggggac
     6601 gataatcggc aggttttttt cggcagcagc cagcatccac gaatttttgg gatctatttc
     6661 gtaatccttt tccattacgc ggctcaacag gagcttatac atgtattcgt gcgggaaata
     6721 ccgttctcct ttagcctggg cgtctttcca gattttttcg atatgatgct ggattttgcg
     6781 aaaggcttct tcttcgggga tgcaggtatc ggttacacgg ttcatgccgt tttgaagcag
     6841 ctcaaattcc tgttccggag tgaggtcacg gtagccggga acccttttgt agtgggagtg
     6901 ggctacgagg ttcatgatat cctcttcgag gtttgcgccg gtgcaggtga tgatctgcac
     6961 tttatcctgc cttatcattt cggccagtga gatgcccagt tcggcagtac tcatggcacc
     7021 gcccatggtt atcatcatct taccgccttc aagcaaatgc tcttcataag cttttgcagc
     7081 atctatcagg gtcgcggaat tgaaatgccg gtaatgatgc tgaatgaatt gtgagatagg
     7141 acctttttcc atactagaac gattgaaaca tgtgcaaagg taagcagaag agcttaatct
     7201 gcggcaataa ttcgatgata tggcaactta gtcccgggca atagaaattt acatccttta
     7261 aatccgtacc tttgcacccc tgtttgaagg aaggaaattt ctcaaattcc cggctgattc
     7321 ccaggaaatt aaaatttttc catcatacgg ccgtctcaaa agttaagata taaaaccgct
     7381 gatttttcat gcaaagtatc cgcaatatag ccattatagc ccatgttgac cacgggaaaa
     7441 ctaccctggt cgataaaatt ctctatgcct gcaaactgtt cagggagaac caggaaacag
     7501 gcgaattgat cctcgacagc aacgatcttg aacgagaacg gggaattacc attacctcga
     7561 agaatgttgc cgttgaatac aaaggagtga agattaacat tattgatact cccggccatg
     7621 ctgatttcgg cggcgaagtg gaacgcgtgc tgaaaatggc cgacggcgtt ttgttgctcg
     7681 ttgatgcctt tgaaggcccg atgccccaga cgcgttttgt tacccaaaaa gccttgtcgc
     7741 tgggacttaa accgattgtt gtcatcaaca aagtggataa ggaaaactgt cgtcccgacg
     7801 aggtgcagga acaggtattc gagcttttct ttaacctgga agccaatgat gaccagcttg
     7861 atttcccgac aatttacggg tcggggaaga acggctggat gagtgaagac tggcaggttc
     7921 aaacgaccga tatcactgcg ttgcttgacg ccatcctgaa atatattccg ccggcgccgc
     7981 agccgcaggg taccttacag atgcagatca cttcgcttga ctataattcc tatgtcgggc
     8041 gtatcgccat tggcagggtt tcgcggggcg agatacatga taccgaggcc gtttcactgg
     8101 ttaagaagga cggggctatt gtcaaatcgc ggatcaagga actctataaa tttgaaggac
     8161 tgggaagggt taaaaccgac gtggtttatg ccggcgatat aggcgccgtg gtcgggcttg
     8221 aaggctttga gataggcgat accattgccg atatcgaaaa ccctgaagct ttgccgggaa
     8281 taaagatcga cgagccgacc atgagtatgc tctttaccat caataattcc ccgtttttcg
     8341 gccgcgaggg taagtatgtt acttcgcgcc atatttcgga ccgtctccaa aaagaaaccg
     8401 agaagaacct tgctctccgg gttgaaccag gagatacgcc cgatcaattc ctggtatacg
     8461 gaaggggaat cctgcacctg agcatcctca tcgaaaccat gcggcgtgaa gggtatgaaa
     8521 tgcaggtagg acagccgcag gtaattatca aggaaattga cggcgtgaag aacgaacccg
     8581 ttgaatcact catcgtcgat acgcctgaag cggttgccgg cagggtgatt gagatagtaa
     8641 cgcaacgcaa aggcgaactc ctggtaatgg agcccaaggg cgatcttcag catcttgaat
     8701 ttcacattcc gtcgcgcggc atcatcggcc tgaggaatgc cgtccttacg gcaacggcag
     8761 gcgaagccat ccttgcccat cgtttcatcg gttttgaacc ctggaaagga cagttgccgc
     8821 aacgccttgc cggtgttatg gtatccatcg aaacaggcat ggcaacagcc tattccattg
     8881 acaagctgct ggaccgcgga cgcttttttg ttgaaccatt tgaagaaatc tatgaaggac
     8941 agatcgttgg cgagcatatc cgcgataacg acctgggcgt taatattgtt aaagggaaac
     9001 agcttacaaa cttccgtact acggccaaag acgatgccgt aagactgccg ccggccgtta
     9061 aattctcgct ggaggaagcg cttgaatata tccaggttga cgaaatggtg gaggtgacgc
     9121 ccaagtcgat gcgcatgcgc aaaatcctgc tgaaggaact tgaccgaaaa agagctgcaa
     9181 aataggcttg ttcatccatc atgatacttt taattagctt tgtagtgtct tgtaaaattt
     9241 agtcagaatg aaactccgct taattgccct gctccttatt tcctgcctgt tcttttcgtt
     9301 taatgcattt tctgaagaag gcatgtggtt gcctgcgctt atcagcaaat ataattatgc
     9361 cgatatgcaa aagatggggc tgaagctcac tcccgatcag atttacagta ttaaccaggc
     9421 ttgcctgaaa gatgccgtcg ttgcccttga tcatggcggt tgcaccggct ccgtcatttc
     9481 aaaaaaaggg cttcttatca ccaaccacca ctgtggtttt gatgccattg ccgatcaaag
     9541 ctcggtcgac catgattatc tcaccgacgg gttttgggct atgcgtcagg aagaggaatt
     9601 gccgcttccc ggtaaaacgg tttcattcct tatcaggatg gaagatgtta cacaggcggt
     9661 attagcggga attccggata tgctggacga agaaagcagg tctgaaaaaa ttgaaaaagc
     9721 catcgacagc atctcaaatg ctgttaaagg ggaaacaaag tacgaggttt ccattgaaag
     9781 catgctggcc ggcaatgaat attacctgtt tgtttaccag acctactccg atatccgcat
     9841 ggttggcgca ccgccttcag gcatcggcaa gtttggcgga gaaaccgata actggatgtg
     9901 gccgcgccac acgggcgatt tttgcctgtt acgggtatat accggaaccg acgggaatcc
     9961 ggccgattat tcaaagcaaa atattccgct atcgcccaaa gaattcctcc ctgtttccct
    10021 ggccggcgtc catgaaggcg atttctcaat gatactcggt tatcccggtg cgaccgaccg
    10081 ttttctaact tcatacggca tccggcaaaa gcttgatcag ctcaacccgt ccgatattct
    10141 tctgaaaact gccaagatgg atatcatccg gcattatatg gagcaaagcg acgctctcag
    10201 gatcgcctat gccagcgaat acgcttacct ggctaatttc cagaaaaaat cgatccagga
    10261 aagcaaagcg ctgaaacggt taaatgtttt tgatgataaa aaaaaacagg aagatgattt
    10321 cgcaagctgg gtaaacgctg atgtggcacg tcaggtcaga tacgggaccg tccttgacag
    10381 cctgaaagcc atctatgacc aaaaaatgaa cgaccgggcc gatgaagccc agaattatct
    10441 gattgaatca gtatcaggca tccaactgct gaattttgcc tatcaggcca acgaactggc
    10501 gagctacctt gccgataacg acagcgttgc agaggctgta gccgattaca ggaacagggc
    10561 caaagagttt tataagaatt atgatctgca ggtagataaa gaaatcatgc agaaaatgtt
    10621 caccctttac cgggacaacc tgccggagga gtattttccc gagtttttcg aaatggttaa
    10681 aaagaagttt ggtaatgatt tcaaccgata tgccgattac gtgtattcaa aatcggtctt
    10741 tgcaacgcct gaaacgtttg aagccttttt aaacaagcct tctttaaaaa agctcgaaaa
    10801 agatcctgct tttacggctg ccaacaatct ggtggcatct tttatcctga cccgttttat
    10861 ccagatgtcg tatgaggaac agtttaaccg gttgcgacgg ctgtatattg ccggaatgcg
    10921 tgaaatgaac cccggcaagg ccttttatcc cgatgccaac agcaccatgc ggcttacata
    10981 tggtcaggta aaatcgtatg tgcctgccga tgcggtggaa tacaactttc aaaccaccgt
    11041 ttccggcgtt attcaaaaag atgacccgtc aaatgctgaa ttcagggttc ctcagaaatt
    11101 gaaggaattg tatgctcgga aggatttcgg ccgatatgcc gaaaacgggg tattgccggt
    11161 ttgcttcctg accacccatg atattaccgg cggtaattcg ggcagtcctg tgatcaatgc
    11221 caacgggcaa cttattggag tggctttcga tggcaattcc gaagccatga gcagcgatat
    11281 aaagtacgac gtaaaccttc agcgaactat tgctgtcgat atacggtatg tactttttat
    11341 cattgataaa tttgccggcg ccggttacct ggttgatgaa atggaactgg cgggaaaata
    11401 aaaagcggat gtgaattgac ggaattgcgc ctgtggctga tggggaaacc ggcagaaatt
    11461 cgggatccgg cttacaggca atcagaaatg aggatggttg aatagttcat tttcctgcca
    11521 gattatcagc aatcaccggt cgtttgattc attccggcga aaagcgccgg ttattgaaat
    11581 gtataactct ttactttatt ctcgtaataa ttgacccctt cttcggtaac gatgccgcgt
    11641 atgaaagtgt cgaacatgac attaaaaagc gtgttaaacg agaattgatc aggcgggaag
    11701 aaatcgggat tgtgcaagtc gatcaggcgg ctgatgtata tgcgggagac cagttccttg
    11761 ctcaggtcag ggcgataaag tccctgattg atccctaacc ggatattcct gcttatttta
    11821 tcaaaaacaa agttgaaacg cagggtgaaa tacttttgct gtaattcagg aaagtattct
    11881 ttaaggtcaa aagaaaagac tggcaatatt ttccgaaagc gatcgctcag cactttgctc
    11941 acttccagca agccgtcaat ggcattatct tcttcaaatg tattatcggt aaagatcgct
    12001 tcaaggttgt ggttctcaaa tttcagaatt tcttcaacca ggccggcttc gttcctgaat
    12061 gtttcggtca gttcgctcag gggcagtcca agatctgcag ataacgcctg aagcgtcaat
    12121 tgtaccggtt tatagtttgc cagttgttca cgtatttttt caataagaac attagtcgaa
    12181 tcctgcataa gaacctgcgt ttgatcgttg cctgaaatgt ggcgaaatta ataaatattc
    12241 acggtttaaa acaggattca ggcaaagagc taactggcac attttcaatg attaaaaatg
    12301 atttttattt ctgaaatacg attttccggt acgtgaattt tcctgcactg ctcaaccgga
    12361 aatagtaaac tcctgacgac aggttaagac cggcgggatt gaactgccgg gtgtattttc
    12421 cgggtatcat cctctcattt ccgataattg tagcaacctt ttgccccatg atatcatata
    12481 cttcgagcgt tacataagcc ggttcccgga ctttgaagga gaaataagcg ctcccggcaa
    12541 acggattcgg ataagttgat tcaacagaaa acggggcatg atcgaccggc gggaggccgg
    12601 tataaatggt atcaaccagc gctgtaagca ccgatagttt ctgttggctc agacgcgtcc
    12661 acatagcatg taccttattg ttgtgagcgc ttattccgtt atagtcgcca aagaagattc
    12721 cggaattcgg cacaaaaggg gtctgactga ttttgaaatt ccggaaggtc tccccgccat
    12781 ctttcgatac ggcaagataa acatcggtgc gggaatcgtt ataattgcgc ctgtcgtaaa
    12841 aaataaacca caggtagcca ttggactggt cgacggtcat ccaggtaaaa aattgatgcc
    12901 tgcctgacgc atcatcatta accctgaccg gcgcgctcca ggtgtttccg ccgtccgtcg
    12961 atttaacaat ccagacatca gtatcatcag cgccgttacg ttgatcggtc cagttgatat
    13021 agatggcacc acgatgtatg ccgttgctca tgtcgcagca ggttaccggc aacccgttgc
    13081 atcttgaaat acccggaata tcgtaattcc atcctccggg gaaatcgctc acaaaaatat
    13141 catggctgag ccaggtattt cccccgtcgg ttgagcggtc gaaaacgata cccgtcgggc
    13201 cggcccagga aacataaatc tggccttccg gcccaacaca cggaacggca ccttctaccg
    13261 tgttgtcatc gtcaaggcag tcgccggctt tttcactgat cctgacaggc tcggaccagg
    13321 taatgccctg atccgaagat tttgagaacc tgataatggt gctgtcgttt ggattccctg
    13381 tgccatatac gtcaaactgg gtccaggtaa gatagatttc attgttaaaa ggattgacac
    13441 atgcccattc cttgtcctgg gctttcgttc cgttaagccc gatgccggtg ccggtgttcc
    13501 aggtttggcc ctgatcgatg gatcgctggc atacgatacg gtcgatgaaa gatccgcccg
    13561 atggccatga cagatgaaaa tagtaataat agcccagggt atcaatgaca acgcaggggt
    13621 cgccccatac attgaactgc gaactcatgg ctacccgatt ccaggattcg cctccatcca
    13681 tactgtggta aatgctttga aggttggcgc ctgccaggag ataatccgta ttttccggat
    13741 tgacgaagat gaccgtctca ttgggctgtg acgtgctgtc gattacaatg ttgcggtatt
    13801 gcgcaaccat cagttcagga atcaacaata acaggaacag aacaggattg caccggatga
    13861 tgggatgaat ggaggagcgt gtcataggcg tgtttattaa cggagggcaa cgtcagattt
    13921 ttatgctgga attatcggta ttgcaaaggt acatgctgac gcagtgaagg aggcaatcct
    13981 tccgattatt attttccgcc tgaaatattc tcctggcggt tgattgataa aaccggagca
    14041 ttatctttgt ttacagtaaa gaacattcct atggcaagat cgaccaaaaa catgaaaatc
    14101 ggtatactta ccgcaggtgg ggactgcccg ggcataaatg ccgctatacg gggcgtcggc
    14161 aaaacggcga tcaccgaata tggaatgaga gttttcggca tttcatccgg gttccggggc
    14221 ctcattgaaa aagagtatac cgaattcgat gaatcacagc tttccggaat cctgaccctg
    14281 ggagggacaa ttttgggaac atcgcgcgat aaacctttca aaaatgatcc gaatgtagaa
    14341 aataaaattc ccaaaataat ccggaacaat tatcaggaaa tgggtttgga tgccctggtt
    14401 gtgatcggtg gaaacggatc catgaaaacg gcgcaaaaac tatatgaaga agggctgaac
    14461 gtaataggcc ttcccaaaac catcgataat gacgtttggg gaacggatgt aagtttcggt
    14521 ttcgattcgg ccgtgtggat tgcaaccgaa gctattgacc ggttgcatac aaccgccaac
    14581 tcgcataaac ggacgatggt tattgagctg atgggccatc atgccggatg gatttctctc
    14641 tattccggcg ttgccggcgg cggggatgta atcctgatac cggaaattaa gtataacatc
    14701 gacatcgtga cagaatacct gataaaaaga catcagaaca agaaaccata ttcaattgtc
    14761 ctggttgccg aaggtataga gaaaccaaag ggaatgcatg ccggaacctt tatagcacag
    14821 gccatacagg ataaaacagg tttcgaaacc cgcgatacca tcctgggcta tatccagcgg
    14881 ggcggttctc cgtcgcccat ggaccgcatt cttgccaccc gttacggcgc ccacgctgta
    14941 gaacttattg ccgacggtaa attcggtgtt atggtaaacc ttaccggcga ccaggtgact
    15001 tctgttccgc ttaccgaggt gtataataaa acgaaacttg ttccccgcga tcattccctt
    15061 atcgacaaag ccaggaaact gggaatttgt tttggcgata aacgggttta acagtagcaa
    15121 ccttatctga tacctgatga atttccggtg acccggaaaa agacctgctc atgaaactta
    15181 ccttgctttt tattgccttg tcccttacag gaaccattgt tctgagcgcc cagaaccctg
    15241 agctgcaaac cgattttgaa aagtcggaag ggttgcggac gccgcggtat gaagaaaccg
    15301 tgaactactg caagaaactc gatgccgctt cgccgctcat cacgtatgcc actttcggcg
    15361 ttagcccgca gggaagggat ttgccgctgc ttatcgccga ttcaaaaggg cgaacggatg
    15421 ttaaggctat ccggaaaagc gggaacatcg tgttgcttat ccaggcttgc atccatgccg
    15481 gcgaacccga tggtaaagat gccgggctga tgctgcttcg cgatatggta acccaaccgt
    15541 cctgccgggc tttgcttgac catgtttccg ttttattcat cccgattttt aacgttgacg
    15601 ggcacgaacg gttcgggcct tacaaccgta tcaaccagaa cggacctgaa gaaatgggct
    15661 ggcggactac tgcccaaaac ctgaacctga accgcgatta tttgaaagcc gatgctcctg
    15721 aaatgaagga ttggctggct ttatacaatt actggatgcc ggatttcctg gtcgattgcc
    15781 acgtgaccga cggcgccgat taccagtatg tgctgaccta tgccctcgaa acgcacggta
    15841 ccatggagca acatctctcg gattgggtcg cgggcacggt ggaaccatac ctggtaaatg
    15901 aaatggataa agccggatac cctgtattcc cttacgtgga gttcagggaa tggcacgacc
    15961 cgcgcagcgg gttggtggtt ggcgccgctc cgcccatgct ctcacaaggg tattgcgcca
    16021 tacagaaccg tccggcgctg ctcatcgaaa gccacatgct caaaccctat aagcaaaggg
    16081 tggaagggac ctatactatg cttcagttgc tgatcgggaa gcttaaccat gaagccggaa
    16141 acctcactaa gcttgtcagg gaagcagatg agtatacctg tgggaaacaa ttcagaagcg
    16201 gggaatttcc ggtgaatttc ggtgaatcgt tgagcgacag cgtcatggtt gatttcaggg
    16261 gaatggagta tacggctgat accagcgacc tgaccggcgg gctgtggttc cggtatgata
    16321 attccaaacc ggtcatatgg aaaattccca tgtttccgca tcctcgaccc gatgtttcgg
    16381 taaagcttcc tgaagcttat atcattcctc ctgaatggtg caatataatc tgcacgctaa
    16441 agcttcacgg tgttgaaatg catgaaattt tgcaggaaca aacgatagag gtctcaactt
    16501 accgtttctc caatgcaaga tggcagcaac ggccttatga aggcaggcat aaagtgcagt
    16561 gtgccgtgaa cgaaacggtt gaaaagagga ctttcccggc aggttcggta gttgtgccga
    16621 tgaaccagcg aacggccagg gttattgccc atttgctgga accccgttcg ccggattctt
    16681 tcgtgagttg gggcttcttc gatgtggttt tcgaacagaa ggaatatttc gaaacctacg
    16741 tgatggagcc ggaagccagg aaaatgatgc tggaaaaccc ggctttgaaa aaggaatttg
    16801 aaaaactcaa agcgtcggac aaaggttttg ccgccgacca atacgcccag ttgatgtggt
    16861 tttacaacaa gacgccgtgg agcgacaagc agtttaacct ttatccggtt ggcaggatta
    16921 tggacgggga aattgtgaag aagttaaaat aggtttcaaa gccgtgcaca gacgttccgt
    16981 cccgataact gccggaaacg gacaaccaga ggaagagata ataaaaccga aaaaaccaac
    17041 acttctaccg atgctatctt cttttcatcc gcacgccggc aggaatactt cctttttctc
    17101 agagcagcgt aaacgttttt tttagttcag cagctgaact ttcaccgact tgcttttgtt
    17161 tacggttcct ctgttttaag gaaagaatcg aagttaccgg aggtaaccag cttaaaaaca
    17221 ttattcgggt aaatatttga aaaggtcaaa ggtaatttgc taccggcatc gctgttccag
    17281 ttgaactcat aagcatgcaa aacgccgtca atatcttcga tataatcaac ctcttgctgt
    17341 tgttgcgtcc gccagaagta cctgttggcc attaaaccgt tattgtgtaa atattttatt
    17401 ctctcgctca ccaggtaatt ttcccaaaga gcgcctttgt cagtcctcag atctaaaggg
    17461 ctgtaattgt tgatcagggc atttcgaata ccgttgtcgt aaaaatatat ttttcttgat
    17521 ttctttaatt cattcctgaa attccggctg aacgaacgca acctaaaaat tacataggtt
    17581 ttttcgagca gttcgatata ccgttgtacg gtttcaatat tggctccaat caaccgcccg
    17641 agttcatggt acgaaacctg cgatccgatc tgaagcgcca gcgcccggag caattgttca
    17701 agcaggtcag gcttccgtaa gtcctggtac ttaaaaatgt ccttataaag gtaacttcct
    17761 gccagtaatc cgagtgtttc ttttacggta gatgctgagt ttaccacatc cggatagctt
    17821 ccgtaaataa gtcgctgctc cagcatcctc aattcatcaa gtcttccgaa gtggttactc
    17881 agctcttcat atgacagagg ataaagataa tattcgaatt ttcgccctgt gagcggttcg
    17941 cttatttctt ccgccagttc caatgccgat gacccgctga ccattaactg tacatcactt
    18001 atatgatcgg tgatgagttt taacgtttta ccgatatttt taacttgttg ggcttcatca
    18061 ataactacta ttttataacc ggccgttaac agcttcaacg ctgatatatt agcatccgtc
    18121 aattgagctt ttacgacagg atcatcggca tcaagccaca gtgtctctga ccgaaaccca
    18181 ctggttattt cccgcagcaa agtggttttt cctgtttgcc ttgcgccaat tactattatg
    18241 gcttttcctt taaaaagcct gtttgttata accggttcca gtttcctttt tatcattc
//
LOCUS       DAOPYV010000063        18139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_3229_length_18139_cov_12.332891, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000063 DAOPYV010000000
VERSION     DAOPYV010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18139
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3229_length_18139_cov_12.332891"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1745
                     /locus_tag="PKI35_08790"
     CDS             <1..1745
                     /locus_tag="PKI35_08790"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNX44516.1"
                     /translation="WILNQIYPAAATPDGANLNIITTTTLTFGMGASTQALFARVGGG
                     IFTKAADVGADLVGKVEAGIPEDDPRNPATIADNVGDNVGDVAGMGADLYESYCGAVL
                     STACLGAAGFISRGVDMQFKAVIAPMVIAAIGILLSIFGIFLVRTKEGAGQKQLLRSL
                     SVGVNSAAIGIAILSWIVLYMLGLPNYTGIWLAMVAGLLAGIGIGQSTEYFTSASYGP
                     TKKIAAAAKTGPATVIISGIGTGMISTAIPVLIVGAAIILSFGFASSWNFSDFSMGLY
                     GIAISAVGMLSTLGITLATDAYGPIADNAGGNAEMSHLGPEVRKRTDALDSLGNTTAA
                     TGKGFAIGSAALTALALLASFLEEARLGMIRFHPDNLLTAQLQNGNLMDVLKAFDVSL
                     MNPRVLVGCFIGGMAAFLFCGLTMNAVGRAAQKMVEEVRRQFREIKGILTGEGTPDYA
                     NCVAISTKAAQREMLFPSMLAIFIPILTGMILGVPGVMGLLLGSTITGFILAVFMANS
                     GGAWDNAKKYIEEGNFGGKHSEPHKAAVTGDTVGDPFKDTSGPSLNILIKLMTMVSIV
                     MVGVTLTFNLMIYF"
     gene            complement(1969..3924)
                     /locus_tag="PKI35_08795"
     CDS             complement(1969..3924)
                     /locus_tag="PKI35_08795"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HNX44517.1"
                     /translation="MNQPVDKPLTHFPVMKYVLALLFAVIVLPLNAGTPDTLRVTTHN
                     RVLVTTDPSQGVKHFPARGIFPDAKTDIRRITMTVTLGCPDSMPCAHWDYLDHIYVRH
                     TGGNNCEPGDYELGRMLTPYGSIFSSNWQYSWTVDVTDFSMLLRGDVEIDYVHSGYEP
                     TDVGWALTIRFDIITGTPVAEPVTITRLWQGDYPYGDPKESIENKLTPIEFKTRGRAS
                     TGRVRIQHTGHGMDEPRGCSEFCSRYRDIKFNGRVVDRRNIWKKCGDNPLYPQGGTWI
                     YDRGYWCPGNLQPADIIDVKLKPGNQTIDIDMEPYRATGKVDAHENISSCLIQYRSPT
                     NANDAAIDNIITPSGSAEFSRINPVCCEPRIIIRNMGSKNLTSAVISYGTIGYKARTY
                     TWHGNLRFNETALVNLRGPVDYGNGNNTFFAMVSLPNGTKDEWVYDNRLTAEFPSPLI
                     LPAEVVIQFHTNNHPADNKLFIMNAFGGIVFNRENTSFKANTTYYDTIRFDPGCYELF
                     LQDSTGDGLEFWFEPEQGRGYLRLLDVKGNLLYNFESDCGNGQHLAFTASPDYVADTT
                     ITKFAFGAYPLMVKNKLTVDCLASKPSDIHVLIRGAENQLLEDHLFLNLRNRETSIDV
                     SHLPSGRYFLEIQVNGERQYKKRFNKE"
     gene            4035..4745
                     /locus_tag="PKI35_08800"
     CDS             4035..4745
                     /locus_tag="PKI35_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNX44518.1"
                     /translation="MGRAFEFRKARKLKRWGNMARTFTKLGKEIEIAVKAGGPDPSGN
                     PRLRLLIQTAKSENMPKDNVERAIKRAVSKDSADYKDVVYEGYAPYGIAVVVETATDN
                     PTRTVANVRSYFNKYGGSLGTTGMTDFLFDRKCTFRVAMKEGLNMEDLELELIDYGLD
                     ETFVDENEIVIYADFQNFGPIQKYLEENKFEIKSFAFERIPHETKELSPEQQAEVEKL
                     LERLEEDDDVTNVFHTMR"
     gene            complement(4843..5415)
                     /locus_tag="PKI35_08805"
     CDS             complement(4843..5415)
                     /locus_tag="PKI35_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNX44519.1"
                     /translation="MPTKIRLQRRGMKGQPYYHIVIADGRAPRDGRFIEKIGTYNPLT
                     RPADIQLNFDRALYWVKCGAQPTETVEAILKYTGVAYMHHLLKGVTKGAMTQEQAQAK
                     FDAWKQEKDAKISSHANRVIQDSKAEDKKRLEAEAKVNEAKAAILAKKRAAEVAAEAK
                     AEEAAAEEAATETAAEAPAAEETATPEAEA"
     gene            complement(5584..6063)
                     /locus_tag="PKI35_08810"
     CDS             complement(5584..6063)
                     /locus_tag="PKI35_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44520.1"
                     /translation="MTEDKTRRLTALADKVFSVKSDPDQLNVDEKVLRKLRNIHPMTI
                     SEYRVEDGPVAWLLVIPTTKELMDQFLDKRINERELFEKTQAGEAYEAVYLCSALVLE
                     EYRGKGIIKSLAIKAIDEIKKDHPIESLFVWPFTEEGDTAAKKISRLVKLPLYKREK"
     gene            complement(6236..7738)
                     /locus_tag="PKI35_08815"
     CDS             complement(6236..7738)
                     /locus_tag="PKI35_08815"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNX44521.1"
                     /translation="MAQIMNDISRTFNEYLLIPGLTRKDCTPDRISLRTPLVKFSRGS
                     SSEIVLNIPFVSAIMQSVSDSNMAIALARNGGLSFVYGSQSIEEQAEMVKKVKKFKAG
                     FVVSDANLTPENTLKDIIDLKARTGFSTVAITHDGTSGGKLLGMVTSRDYRPGRDSLD
                     KKVKDFMTPFSKLVTGKNGISLDEANDIIWDNKINTLPIIDENQCMKYFVFRKDYENH
                     KDNPNELLDNHKRLLVGAGINTRDYKERIPRLLNAGVDILCIDSSDGFSEWQKETIRF
                     VRDHYGEKVKIGAGNIVDEEGFNYLTEAGADFIKVGIGGGSICITREQKGIGRGQATA
                     LIEVARARDEYFNKTGQYIPICSDGGIVQDYHMVLALAMGADFLMMGRYFARFDESPT
                     KKLLVGGNYVKEYWGEGSNRARNWQRYDMGGNASLKFEEGVDSYVPYAGKLKDNVEIT
                     LGKIKSTMCSCGALTIPELKQTAKITMVSSTSIIEGGAHDVILKETKRES"
     gene            8129..11797
                     /gene="dnaE"
                     /locus_tag="PKI35_08820"
     CDS             8129..11797
                     /gene="dnaE"
                     /locus_tag="PKI35_08820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNX44522.1"
                     /translation="MPQFSHLHVHSQYSLLDGASEISAMYKKAVADNMPGLALTDHGN
                     MFGIFQFVAEAEKQNKAKTIIKPIVGCEFYVVDDRHRKKFGGGERDIRYHQILLAKNA
                     EGYANLVKLCSLGFIEGFYSKYPRIDKELIVRYHKGLIATTCCLAASVPRAIMRKGEA
                     EGEKEFKWWLDIFGEDYYIELQRHNIPEQNEVNEVLMRYARKYNVKMIASNDSHYVEK
                     DNYNAHDILLCVNTGAKQATPTFRDYGDDDGGTNGRRFAFPNDQFYFKSTSEMCEVFE
                     DVPEAIDNTNEVVGKIEILNLKKDILLPNFPIPDAFKLYDTPVLINNKELSADLRNQW
                     EYLKHLTMEGARRRYSELTSDLIDRINFELQTIHDMGFAGYFLIVRDFIKAGRDMGVF
                     IGPGRGSAAGSVVAYCIGITSIDPIKYDLLFERFLNPERISMPDIDTDFDDQGRQKVI
                     DYVAQKYGQNQVAQIVTFGTMAAKTSIKDVARVLDLPLPESNALAKLVPDKPGTSLKR
                     VLHAPLHKSDGAASLEEKEGLPGDDLENVKHLREAYSYTGDDPTGVLKSTILHEAEVL
                     EGSVRNTGIHAAGVIIAPTDLSDLIPVCTSKDSNLWITQYEGSIIESAGVIKMDFLGL
                     KNLTIIRDALQLIEQNHGVKLDIDGIPLDDPKTFDLYQHGDTNGTFQFESPGMQQWLR
                     QLKPDTFTDLIAMNALYRPGPMEYIPNYVARKQKREPVTYDLPEMEDILKDTYGITVY
                     QEQVMLLSQKLAGFSKGKADELRKAMGKKLREKLDKMKPEFIEGGNQRGHPADKLEKI
                     WSDWEAFASYAFNKSHSTCYAFVAYQTAYLKVHYPAEYMAALLTSNLNNLENVTFYID
                     ECRRMGIRVLGPDVNESDLNFTVNKNGEIRFGLMALKGVGESAAESIISERKEKGPYT
                     NFFDFVKRVNLRTVNKSSVESLSQAGAFDSFEGMHRAQYFAKDHAEDATFIEKSLRFG
                     SSFQAISQSMQVSLFGDAMNQEIPDPPVPYCETWSNLEKLKREKAIAGFFISGHPLDD
                     YRSEINSLCNINIKSIKENLNALQPRDFIFGGIISAVRHDTLKNGNGWGRFTIEDFED
                     SYEGALFGEDYLKFKHLLEEDKALIIKARVQERYGRQGQFELKFIQMTLLSEAFEKYA
                     KQLLIQIPLTTMNDSFVNGFSKLVKSAKGDCKLRVQVFDLDENLRVDMLSPKHKVKCS
                     TVVHELGKLGMAGFKVTM"
     gene            11960..12277
                     /gene="trxA"
                     /locus_tag="PKI35_08825"
     CDS             11960..12277
                     /gene="trxA"
                     /locus_tag="PKI35_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNX44523.1"
                     /translation="MALQFTDATFEEQVLKSNIPAVVDFWAEWCGPCRTIGPLIEELA
                     KEYEGKVMIGKVDVDANPDIVARYSIRNIPTILFIKGGEVIDKQVGAVPKSVLAGKLE
                     KLL"
     gene            12469..13404
                     /locus_tag="PKI35_08830"
     CDS             12469..13404
                     /locus_tag="PKI35_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNX44524.1"
                     /translation="MSEQLDTSRLQPFGNLEFIARQVVEGFIVGLHKSPFHGFSVEFS
                     EHRLYNPGESIRHIDWKLYGRTERLYVKRYEEETNLRCQIVIDNSSSMYFPVRERLSA
                     DNPNKVIFSIYSAASIMNLLHRQRDAVGLSLFSNDVELHTQAKSSSVHYKYILGELEK
                     LLTPVSLQANKPSMVAESLHNIAENIHKRSMVILFSDMFSAGYDHAALFDALQHLKYN
                     KHEVILFHVVDKLHELDFGYDNRPHRFVDMETGEEIKLQPSQVRTSYVRAVNDYIETI
                     KMKCGQYRIDFVEADIKRGFRQVLFPYLLKREKML"
     gene            complement(13491..14393)
                     /locus_tag="PKI35_08835"
     CDS             complement(13491..14393)
                     /locus_tag="PKI35_08835"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HNX44525.1"
                     /translation="MKRILTILIILLVTTAVRSQTSDLAFFTQNGEPFSVVLNGMLQN
                     DSPETYIKITGLPAPSYKVKIIFLNKRLGEINKTVFVTAGNETTYSVRKGKKNRYVLR
                     MMNTCPLAQAPAPSAGQTVTGYPPQGTSQGTNTTTTVINNTTINNNVNVSATGVGNTV
                     TISNDNQGAGSQQVNRNSDGQHQHGYDPNLYIMPGYNGPYGCPWPMAEADFIQAKQSI
                     ESKTFEDSKLTIAKQIAGSNCLLCSQVKEIMLLFTYESTKLEFAKYAFPFTLDQGNYF
                     RVNDAFTYESSIDELNEFLHSGRK"
     gene            complement(14492..15169)
                     /gene="mtgA"
                     /locus_tag="PKI35_08840"
     CDS             complement(14492..15169)
                     /gene="mtgA"
                     /locus_tag="PKI35_08840"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNX44526.1"
                     /translation="MRLIVKYLRFAILFFLISTIVVALLFRFINPPATPLMMIRVVEQ
                     LAEGESPRMQYNWIPLEKMPKNLPLAVIASEDNKFMTHHGIDLKAIQKAQKLNKRGRK
                     LRGASTISQQTAKNVFLWPARNWVRKGLEVYFTGLIELTWGKERIMEVYLNVIEMGDG
                     IYGAEMAAEQYFHKPAEKLTRSECAAIAAVLPGPRKWRPDRPTPYIQRKISAISSMMY
                     KVERPGW"
     gene            15456..16769
                     /locus_tag="PKI35_08845"
     CDS             15456..16769
                     /locus_tag="PKI35_08845"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysine ketoglutarate reductase
                     /saccharopine dehydrogenase family protein"
                     /protein_id="HNX44527.1"
                     /translation="MTKLIGIRHEDKYAMERRVPLIPRHVERLIKTKKLDIVVQSSDK
                     RIFPDEEYQKAGAAIADNLKNCDVIFGVKEIPETFFETGKTYIFFSHVIKGQSYNMPM
                     LRRMMELKCNLIDYEKVVDEQGKRLIFFGRYAGLAGMINSLWSLGLRLKEYGTDTPFL
                     NLKQARHYSSLAEARNAISAVGQEIAEKGLPRELRPFTVAFTGYGNVSQGAQEILGLL
                     PVKEISPEKLLELEKREHLPNNLIYKVVFKEEHLVEPVEPGTEFDLHDYYANPQHYRG
                     TFSKYLPHLSMLINCIYWDARYPRLVTKEQLAGLFRDGRPKLTVVGDISCDVNGAVEF
                     TEKATEIEDPIFVYDPFIQSITMGHQGEGMLVMAVDILPSELPRDSSSGFSDVLMNFV
                     KPIADCDFNDEFEDLDLPRAVKRALILHNGELTPDYRYLEEYINA"
     gene            16896..>18139
                     /locus_tag="PKI35_08850"
     CDS             16896..>18139
                     /locus_tag="PKI35_08850"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNX44528.1"
                     /translation="MKKVLILGAGLVVRPIVKHLLSKGIHVTVATRTKSKAEEMIDGH
                     PNGTAVAWTVEDEAKLSQMVSEHDLTVSLLPYVHHLLVAKHCIANKKNMVTTSYVKPE
                     MQALDAAAKEANIIILNESGLDPGIDHMSAMRIIDNVHKKGGKIEEFYSLCGALPAPE
                     LADDNPFRYKFSWSPKGVVMAGNNDAMYLKQGKVVKIPTQDLFKNPKQVDFPKVGKLE
                     VYPNRDSIMYKEIYGIPETTTIMRGTFRYLGWCETLDLLKKLKLISYDKVNLEGKTYA
                     QFVAMQIGAADASDIRKKVAAFAGIDVNAYALNAMEWLGYFSNEPVGRVEDSPFEVTS
                     DMMISKMGLGMNERDMVVMQHNFLAVYPDGSKEIIRSRMLDFGTPATDTSIARTVALP
                     AAIAVEMILEGKIPVKGVFRPVL"
BASE COUNT         4631 a         4287 c         4436 g         4785 t
ORIGIN      
        1 tttggattct caaccagata tatccggctg ctgccacacc ggatggcgcc aacctgaata
       61 tcatcactac cacaaccctt accttcggaa tgggagcttc aacgcaggct ttatttgccc
      121 gtgttggcgg tggtattttc acaaaagctg ccgatgtagg cgccgacctg gttggaaaag
      181 tggaagccgg tatcccggaa gatgatcccc ggaaccctgc tactattgcc gataacgttg
      241 gcgataatgt tggcgacgtc gccggtatgg gagccgacct ttacgaatcc tattgcgggg
      301 ccgtattatc taccgcatgc cttggcgctg caggttttat cagccgtggc gttgatatgc
      361 agtttaaggc tgttattgcc cccatggtga tagcggctat cgggatttta ttgtcgatct
      421 tcggcatttt cctggttcgg accaaagaag gcgccggaca aaaacaactg ctgcgttcac
      481 tgagtgtggg cgtaaattcg gcagccattg gtatcgctat attatcatgg atagtattgt
      541 atatgctcgg attgccgaac tatacgggaa tatggctggc tatggtcgcc ggattgctgg
      601 caggaatagg catcggacag tcgaccgaat actttacctc agcatcatac ggacctacga
      661 aaaagattgc cgccgcagca aaaacaggtc ctgcaaccgt gatcatatca ggtatcggga
      721 ccggaatgat ctcgacggct attccggtgc tcatcgttgg ggccgccatt atcctttcgt
      781 ttgggtttgc ctcctcctgg aatttttcag atttcagcat gggactttac gggatcgcaa
      841 tatcagcagt agggatgctt tccaccctcg gtattacact ggctactgat gcctacggac
      901 cgattgccga caatgcaggc ggtaatgccg aaatgagcca tctggggcca gaagttcgca
      961 aacgcaccga tgccctcgat tcgctcggaa ataccactgc agccaccggc aaaggctttg
     1021 ccattggttc ggcggcttta acggcccttg ccttgctggc ttccttcctc gaagaggctc
     1081 gtttgggtat gatccgtttc catcccgaca atctgcttac agcacaactt caaaacggca
     1141 acctcatgga tgttctcaag gcatttgatg tgagcctcat gaatccccgc gtgttagtcg
     1201 gttgctttat cggaggcatg gcagccttcc tgttctgcgg attgaccatg aacgcggttg
     1261 gacgggcagc ccagaaaatg gttgaggaag tacgtcgcca gttccgtgaa attaaaggca
     1321 ttctcaccgg cgaaggaact cccgactatg ccaattgcgt agctatttca accaaagctg
     1381 cacaacgcga gatgctgttc ccctccatgc tggccatttt catcccaatc cttaccggga
     1441 tgatcctcgg ggttccgggt gttatgggtc tgctcctcgg cagcaccatc acaggattta
     1501 ttctggctgt gttcatggcc aattcgggcg gcgcatggga caatgccaag aaatacattg
     1561 aagaaggcaa tttcggcggt aaacacagcg aacctcacaa agctgccgtt accggcgaca
     1621 ccgttggaga tccgttcaag gatacttcag gccccagcct caatatcctg atcaaactca
     1681 tgaccatggt ttcgattgta atggttggcg ttacgcttac gtttaacctg atgatatatt
     1741 tttaaaatca ctggagatcg cgaaaagaag aggctgccct cacgggcagc ctcttctttt
     1801 ttccggttaa ggcattcttc ctgtatcagt ttcacctgct gacctgaaaa cgatggatgg
     1861 gcgggataag gaggaagtta tttccgccag accggccagt ttcaactcag gcagtaattt
     1921 cgccttatcg taccgaatga gtgtgcttat tcactcaccc ggttttgttt attccttatt
     1981 gaaccgtttc ttatactgac gttccccgtt aacctgaatt tccaggaaat atcttcctga
     2041 aggcagatga cttacatcga tggatgtttc acggttgcgg aggttcagga aaaggtggtc
     2101 ttccagcaat tggttctccg cacccctgat caatacatga atatcggagg gctttgaagc
     2161 caggcagtca acagtcagtt tatttttaac cattaatgga tacgctccga aggcgaattt
     2221 ggtaattgtt gtgtcggcca cataatcggg cgaagcggta aaagccagat gctggccgtt
     2281 gccgcagtcg ctctcgaaat tgtacagcag gttgcctttt acatcgagaa gccggagata
     2341 accccggcct tgctcgggct caaaccagaa ttcgaggcca tcgccggtgc tgtcctgcag
     2401 gaataattca tagcaacccg gatcgaaacg gatggtgtcg taataggttg tattggcttt
     2461 gaagctggtg ttttcacggt taaatacaat tcctccgaaa gcgttcatga tgaacagctt
     2521 gttgtccgca ggatggttgt tggtatggaa ctggatgacg acttcggcag ggagtatcaa
     2581 cggagaagga aattcagccg ttagcctgtt atcgtatacc cattcatctt ttgtcccgtt
     2641 tggtaaactg accatggcaa agaatgtgtt attcccgttc ccgtaatcaa ccggtccccg
     2701 gaggtttact aaagcagttt cattaaaacg gaggtttccg tgccaggtat atgtccgggc
     2761 cttatagcca atggtgccgt atgagattac tgccgaggtc aggtttttgc tgcccatgtt
     2821 ccggattata attctgggtt cgcagcacac cggattgatc ctgctgaatt cagccgatcc
     2881 ggaaggcgtg atgatattat cgatggcggc atcgttggca ttagtaggcg aacggtattg
     2941 tatcaggcag gatgaaatgt tttcgtgggc atccactttg ccggttgcac ggtatggttc
     3001 catgtcaatg tcgatggtct ggttgcccgg cttcaactta acatcgatga tatcggccgg
     3061 ctgaaggttg ccgggacacc agtaaccgcg gtcgtagatc caggttccgc cctggggata
     3121 aagcgggttg tcgccgcatt tcttccagat gttcctgcgg tcgacaacgc gaccgttaaa
     3181 ttttatatcg cggtagcgcg agcagaactc gctgcaacct ctgggctcat ccatgccatg
     3241 accggtatgc tgtatgcgta ccctgcctgt ggatgccctg ccccgggttt taaattctat
     3301 cggcgtaagc ttgttttcga tgctttcttt tgggtcgccg taaggataat caccctgcca
     3361 gagcctggtt attgttacag gctcggctac cggcgtaccg gtgatgatat cgaagcggat
     3421 ggtgagcgcc cagcctacat ctgtcggctc ataaccggaa tggacataat caatttccac
     3481 atcaccccgc agcagcatcg aaaagtcggt tacatccacc gtccagctat attgccagtt
     3541 gctgctgaaa atactgccgt aaggggtaag catacgcccg agttcgtaat ctcccggttc
     3601 gcaattgttt ccgccggtat gtctgacgta aatatggtcg agataatccc agtgcgcgca
     3661 gggcatactg tcggggcacc ccagcgtcac agtcatggta attctgcgta tatcggtttt
     3721 agcgtcggga aaaattccac gggcgggaaa atgctttacc ccctgggagg gatcggttgt
     3781 aaccaggacg cggttgtgcg tggttacccg gagcgtatcg ggcgtgccgg cattcagcgg
     3841 taaaacgatg acggcaaaca ggagtgcaag gacatacttc atgacgggaa aatgggttaa
     3901 cggtttatca acaggctggt tcatgcttaa atttaaggca ggtttttcaa attccggcaa
     3961 agaaagctat ttttgcccct ttttccggat atgccgggaa ttttttatcc acagcaaagc
     4021 aaacactacg acaaatgggc agagcatttg aatttcgcaa agcgcgcaaa ctgaaacgtt
     4081 ggggcaatat ggcccggacc tttaccaaac tggggaagga aattgaaatt gcagtgaaag
     4141 ccggaggccc cgacccatca ggaaatccga ggctgaggct gcttatccag acggcgaaaa
     4201 gtgagaacat gccgaaggat aatgtggaaa gggccatcaa aagggctgta tcaaaggact
     4261 ctgccgatta caaagatgtt gtttatgaag gctatgcccc ttacgggatt gccgtggtgg
     4321 ttgaaacggc caccgacaat cctacccgga cggtcgccaa tgtccgcagt tatttcaaca
     4381 aatatggcgg aagcctcgga acgacgggaa tgaccgattt tcttttcgac cgcaaatgta
     4441 ctttcagggt agctatgaaa gaggggttga atatggaaga tcttgaactt gaactcattg
     4501 actacggcct tgacgaaact tttgtggatg aaaacgagat cgtgatttat gccgattttc
     4561 agaatttcgg gccaattcag aaatatctgg aagaaaataa atttgagata aaaagttttg
     4621 ccttcgaacg tattccgcat gaaaccaaag agctttcccc ggaacagcag gcagaggtcg
     4681 aaaaacttct ggaaaggctt gaagaagatg acgatgttac caacgtgttt cacacgatga
     4741 ggtaacggtt cgtgaaataa aattccggat aacaagccgg gaaattcaat aaagaaggct
     4801 gcctttgggg cagccttctt ttcgtatttc agaagccttt ggttatgctt cggcttcggg
     4861 agtagcagtt tcttcagcgg caggagcttc ggcggctgtt tcggtagcgg cttcttcagc
     4921 agcagcttct tcagctttag cttcggcggc aacttcggca gcgcgtttct tggccaggat
     4981 ggcagccttg gcttcgttaa ccttggcttc ggcttcaagg cgttttttat cctcggcttt
     5041 cgagtcctgt attacgcggt tggcgtggct gctgattttt gcgtcttttt cctgtttcca
     5101 ggcatcaaat ttggcctgtg cctgttcctg ggtcatagcg cctttggtaa cgcctttcag
     5161 caggtggtgc atgtaagcga caccggtgta cttgaggatg gcttccacgg tttcggtagg
     5221 ctgtgcgccg cacttcaccc aatacagggc gcggtcaaag ttaagctgaa tgtcggcagg
     5281 ccgggtaagg ggattgtacg tgccaatctt ctcaataaaa cgcccgtcac gtggtgcacg
     5341 accatccgcg ataacaatgt gataataagg ctgacctttc ataccacgtc tttgtaatct
     5401 aatcttggtt ggcatgtttt aatggatttt ggtgattgtg aatgaatagc tttcttgtct
     5461 gaaaaatcaa atgcatgcga tttttcgtca aatcgggtgc aaagatcgta aaaaatttga
     5521 ttacaggctg atgcccgagg aaaatttatc atgataaatt tgtcagcact attctccggc
     5581 gctttacttt tcccgcttat aaagcggcag tttcacaagc cgtgatattt ttttcgccgc
     5641 tgtatctcct tcctctgtga aaggccacac aaacaggctt tcgatcgggt ggtccttttt
     5701 tatttcatcg atggctttta tagccagact ttttattatt ccttttccgc ggtattcttc
     5761 gagcaccaga gcagaacaaa ggtacacggc ttcgtatgcc tcaccggcct gagtcttctc
     5821 aaacagttcc cgttcattga tccttttatc gagaaactgg tccatcagtt cttttgttgt
     5881 cgggattacc agaagccagg caaccgggcc gtcctccacc ctgtattcgg atatcgtcat
     5941 cggatggata ttccgtaatt tcctcaatac tttttcgtca acgttcagtt gatccggatc
     6001 gcttttcact gaaaatacct tgtccgcgag tgctgtcagc cgtctggttt tatcttccgt
     6061 catgcttctg cttaacctgt ttattgcaca ttccggatac ggcggtttgt cctggttttc
     6121 gcgacagaat ccccggactt cctgccagtc gcctgaaaag gaagctgcga atgagtattt
     6181 agttggacta ttgtatggcg cctgaggtgt ggtatctgcc gccggtcagc aggcgttaac
     6241 tttcccgttt tgtttctttc aggattacat cgtgggcgcc gccttcgatg atacttgttg
     6301 aagaaaccat cgttattttt gctgtttgct ttaattccgg tatcgttaag gcgccgcaac
     6361 tgcacatcgt cgactttatt ttacccagtg taatttcaac attgtcctta agctttccgg
     6421 cataaggaac atagctgtcg acgccttctt caaacttcag cgaagcgttg ccgcccatat
     6481 catagcgctg ccagttcctg gcacggtttg atccttcgcc ccaatactcc tttacataat
     6541 tgccgcccac cagcaatttc ttggtcgggc tttcatcaaa tctggcaaaa taccttccca
     6601 tcatcagaaa atcagctccc atggccaagg cgagcaccat atgatagtcc tgtacgattc
     6661 ctccgtcgga acaaatcgga atatactggc cggttttgtt aaaatattca tcgcgcgccc
     6721 tggcaacttc gatcagggcg gttgcctgcc cccttccgat ccctttttgt tcccgggtga
     6781 tacaaatgga gcctcccccg attccgactt tgataaaatc agccccggct tcggtcagat
     6841 aattaaatcc ctcttcatca acaatatttc cggccccgat ttttaccttc tcgccataat
     6901 gatccctgac aaacctgatc gtttccttct gccattccga aaatccatcg gatgaatcga
     6961 tgcataaaat atcgactccg gcattcagga gcctgggaat gcgttcttta taatcccggg
     7021 tgttgatgcc ggccccgacc agcaaccttt tatggttatc cagtaattcg ttggggttgt
     7081 ctttgtggtt ttcatagtct tttctgaaca cgaaatactt catacactga ttttcatcaa
     7141 tgatggggag cgtgttgatt ttattatccc agatgatatc attggcctca tcgaggctga
     7201 tcccgttttt acctgtaaca agttttgaga agggagtcat gaaatctttt acttttttat
     7261 caaggctgtc cctcccaggc cggtaatccc ggctggtaac cattcccagt aatttaccgc
     7321 ccgatgttcc gtcatgggta atcgcaacgg tagagaatcc ggtcctggct ttcagatcaa
     7381 ttatatcttt cagcgtgttt tcaggcgtca ggtttgcatc gcttacgacg aatcctgcct
     7441 taaacttttt tacttttttt accatctcag cctgttcttc gatcgactgg gagccataaa
     7501 caaaggacag accgccattc ctggccaagg cgatagccat attcgaatcg gaaacagatt
     7561 gcatgatggc tgacacaaaa ggaatattca ggacaatttc tgaactgctt cctcttgaga
     7621 atttcaccag cggcgtacgt aaacttatcc tgtcaggggt gcaatctttt ctggtgagcc
     7681 cgggtatgag caaatactcg ttgaatgtcc ttgaaatatc attcattatc tgtgccatgt
     7741 gatgtacttt aaaagtttag caaaaataga aacatctgag cacaaggagt agcggttcag
     7801 ggattttaaa cagcagaact gttatttttg cggagccgtt gtgctgaatg ccaccctgtt
     7861 tatggccaga agtgcctgac gcgaaaactg acggctccgt ttccgggctt ttccaatccc
     7921 gggcatgacc gccggattct gatgaccatg cttcacgcag tttgaatcgt tgtcgtgcag
     7981 gtgaatgaag gccacgatag ccttcaaggg taatcttatc caaaaattct atcctttttt
     8041 atcaacattt cttttgaacc tctttccgat tgggtatttt agcaaaactt acaatgaaat
     8101 tttaattgcc tgattaccaa tttgccccat gccccaattc agtcacctcc acgttcactc
     8161 tcaatactca ctccttgacg gcgcttccga aatctctgcc atgtacaaaa aggccgtcgc
     8221 ggataatatg cctggtctgg cgcttaccga tcatggcaac atgttcggta ttttccagtt
     8281 tgtggccgaa gctgaaaaac aaaataaagc aaagactatc atcaaaccca tcgtcggctg
     8341 cgagttttat gttgtcgacg accgtcaccg gaaaaagttc ggcggcggag agcgcgacat
     8401 ccgttatcac cagattttac tcgctaaaaa tgctgaagga tatgctaacc tggtgaaact
     8461 gtgctcgctg ggttttattg agggctttta cagcaaatac ccgcgtattg acaaggaact
     8521 gatcgtgcgg taccacaagg ggctgatcgc caccacctgc tgcctggctg cgtctgtgcc
     8581 ccgcgccatt atgcgcaagg gcgaagccga aggtgaaaag gaattcaaat ggtggctcga
     8641 tatcttcggc gaagactatt acatcgaact ccagcgccat aacatcccgg aacaaaacga
     8701 ggtgaacgaa gtgctgatgc gttatgcccg taagtacaat gtgaaaatga tcgcgagcaa
     8761 cgactcgcac tatgttgaaa aagacaatta caacgcccac gatattctgc tttgcgtaaa
     8821 caccggcgca aagcaggcca cgcctacttt cagggattac ggcgacgatg acggcggcac
     8881 aaatggcagg cggtttgcct tccctaacga ccagttttat ttcaagtcaa cctccgaaat
     8941 gtgcgaggtg tttgaggatg taccggaagc tatcgataat acgaacgaag tagtcgggaa
     9001 aattgagata ctgaatctga aaaaggatat cctgttgcca aattttccca ttcctgatgc
     9061 tttcaaattg tacgatactc ctgtcctgat caacaataag gagttatctg ccgatttgcg
     9121 aaatcagtgg gaatacctga agcatcttac catggaaggc gccaggcgga ggtactccga
     9181 actgacatcc gatcttattg accgtatcaa tttcgagctc cagaccatcc acgatatggg
     9241 ctttgccggt tacttcctca tcgtacgcga cttcattaaa gccggccgcg atatgggcgt
     9301 attcataggc cccggacgtg gttcggctgc cggcagtgtg gtggcatact gcatcggtat
     9361 taccagcatc gacccgataa agtatgacct gctgttcgaa cgtttcctga atcccgaacg
     9421 tatcagcatg cctgatatag ataccgactt tgatgaccag ggacggcaga aggtgataga
     9481 ttatgttgcg cagaaatacg ggcagaacca ggtagcgcag atcgttactt ttggtacgat
     9541 ggctgccaag acaagcatca aggatgtggc gcgggtcctt gaccttccgc tgcccgagag
     9601 caatgccctc gccaaattag tgcccgataa acccggcact tcactgaagc gggtactcca
     9661 tgcccccctg cataaaagtg acggcgccgc ctccctcgaa gaaaaggaag gccttcccgg
     9721 cgacgacctc gaaaatgtaa aacacctcag ggaagcctat tcatacaccg gcgatgaccc
     9781 gacaggcgta ttaaaaagta ccatcctgca tgaagctgaa gtgctcgaag gctcagtgcg
     9841 taatacaggc attcatgcag ccggcgtcat catcgcgccc actgacctga gcgacctgat
     9901 ccccgtttgc acttccaaag attccaatct gtggattacg caatatgaag gaagcatcat
     9961 cgaaagcgcc ggcgttatca agatggactt cctggggctt aagaacctta ccattatcag
    10021 ggacgccctg caactgattg aacagaacca cggcgtaaaa cttgatatcg atggaatccc
    10081 gctcgacgat ccgaaaactt ttgaccttta ccaacacggc gataccaacg gcactttcca
    10141 gtttgaaagt cccgggatgc agcagtggct caggcaactg aagcccgata cctttaccga
    10201 ccttatcgcc atgaatgcgc tctatcgccc gggcccgatg gaatatatcc ccaactatgt
    10261 ggcccgcaaa caaaagcggg aaccggtaac ctacgatctt cccgagatgg aggatatcct
    10321 gaaggataca tacgggatta cggtatacca ggaacaggtg atgctgcttt cgcagaaact
    10381 ggcgggattc agcaaaggca aggctgatga actgaggaag gcaatgggca agaagcttcg
    10441 cgaaaagctg gataaaatga agccggaatt catcgaaggc ggcaatcagc gcgggcatcc
    10501 tgcggataag ctggaaaaga tatggtccga ctgggaagct tttgcatcgt atgctttcaa
    10561 caagtcgcac tctacctgtt atgcttttgt ggcctatcaa acggcgtacc ttaaggtcca
    10621 ttacccggca gagtatatgg cagccctgct caccagcaac ctcaataacc tcgagaatgt
    10681 aaccttttac atcgacgaat gccgcagaat ggggataagg gtgctgggac ctgacgtaaa
    10741 tgagtcggac ctgaacttca ccgtgaataa aaacggcgaa atccgtttcg gactgatggc
    10801 gctgaaagga gtaggcgagt cggctgccga aagcatcatt tccgaaagga aggaaaaagg
    10861 gccctacaca aacttttttg attttgtgaa acgcgtaaac ctccgcactg ttaataaaag
    10921 cagtgtagaa tcgttgtcgc aggcaggcgc attcgacagt ttcgagggaa tgcatcgcgc
    10981 ccagtatttc gccaaagatc atgcggaaga tgcgactttc atcgaaaaat cgctgaggtt
    11041 cgggagtagt ttccaggcaa tcagccagtc gatgcaggta tcgctgttcg gagatgccat
    11101 gaaccaggaa attcccgatc cgccggttcc atattgcgag acatggtcaa acctggagaa
    11161 acttaaacgt gaaaaagcca tcgccgggtt ctttatttcg gggcatcccc tcgatgatta
    11221 ccgttccgag atcaactcgt tgtgcaatat taacatcaaa tccatcaagg aaaacctgaa
    11281 tgcgttgcaa ccccgcgatt ttattttcgg cggaataatc tcggccgtca ggcacgatac
    11341 cctgaaaaac gggaacggct ggggccggtt caccatcgag gatttcgagg attcatacga
    11401 aggcgccctg tttggcgagg attacctgaa atttaaacac ctgctcgaag aggacaaagc
    11461 gctgatcatc aaagccaggg tacaggaaag atatggaagg caggggcaat ttgagctcaa
    11521 atttatccag atgaccttat taagcgaggc atttgagaaa tatgccaaac aattgctcat
    11581 ccagattccg ttgacaacca tgaacgatag ctttgtaaat ggcttctcga agttggttaa
    11641 atcagcaaag ggcgattgta aactcagggt ccaggtattc gatttggacg agaacctgag
    11701 ggtagatatg ctatcgccca aacataaagt aaaatgctcg accgttgttc atgaacttgg
    11761 gaaacttggc atggcaggat ttaaagtaac gatgtaaacc ccggaaacgg aaaagtttac
    11821 agcctgccgc agataaaaac gccgcatcag gcgatatgag ccgcaattat gttgcaggca
    11881 taagcagaat gaaatatgga tttatttact tttgtatctg aatcccaaaa aacctaatat
    11941 cactaaaaag aaaattatta tggcactaca atttaccgat gcgacatttg aagaacaggt
    12001 attaaaatcg aatataccag ccgttgtcga tttctgggca gaatggtgcg gtccctgccg
    12061 gacaatcggg ccgcttattg aagaattggc aaaggaatat gaaggcaaag tgatgatcgg
    12121 taaagttgat gttgacgcga atcccgacat tgtagcacgt tatagtatcc gcaacattcc
    12181 cacgatactc tttattaaag gcggagaagt gatagataaa caggttggcg ctgttccgaa
    12241 atcggtgttg gcaggcaaac ttgaaaagct gctctaaatt gttccgggcc tgaaacctga
    12301 agatatgact tgctccggga atatttccgg agccattctt ttatcgttga tggaaaaatg
    12361 atgcgaccgt ttgatgttac atcgttgacc tgagcctgaa ttcaatgttt ttgtttgtat
    12421 agttcttccg gttgatctga aacatcccaa ccttaaatcc aggaaacttt gtccgaacaa
    12481 cttgatacca gccgtctgca accatttggc aatcttgaat tcattgcccg tcaggtagtt
    12541 gagggtttca ttgtcggcct tcacaaaagc ccgttccatg gtttttcggt tgagttttcc
    12601 gaacacaggc tttataaccc gggcgaatca attcgccata tcgactggaa actatacggg
    12661 cgaacagaaa ggctgtatgt gaaacgttat gaggaagaga ccaatctccg ctgccagatt
    12721 gtgatcgata attcttcatc aatgtatttt cccgtccgcg aacgcctttc ggcggataac
    12781 ccgaataagg ttatcttctc gatttattcg gccgccagca tcatgaacct gctgcaccgg
    12841 caacgcgatg ccgtcgggct aagccttttc agcaacgatg ttgaactgca tacgcaggcg
    12901 aaatcaagtt cggtacatta taaatacatc ctcggcgaac tcgaaaaact gctgacccct
    12961 gtttcgcttc aggccaataa gccttccatg gtggctgaat cgcttcacaa tatcgctgag
    13021 aatattcata aaaggtcaat ggtgatcctt ttcagcgata tgttttctgc cggttacgac
    13081 catgcagcgc tgttcgatgc cctgcagcat ctcaaataca acaagcacga ggtcatcctt
    13141 tttcacgttg tggataaatt gcatgaactg gacttcgggt atgataaccg tccgcaccgg
    13201 tttgtagata tggaaacagg tgaagaaatc aaactgcagc catcccaggt tcgcacatcc
    13261 tatgtcaggg cagtcaacga ttatattgaa accataaaaa tgaaatgcgg acagtaccgg
    13321 atcgattttg ttgaggcgga tataaagcgg ggattcaggc aggtgctttt tccttattta
    13381 ctgaagcggg aaaaaatgtt gtagccgtta cttccggatg gataaagcga attccgatgt
    13441 ttcgtatata ttcctgtgaa tgctgaaacc gctcagggtt ttagaggccg ttatttcctt
    13501 cccgaatgca ggaactcatt taattcatcg atagacgatt cataagtaaa agcgtcgttt
    13561 accctgaagt aattgccttg gtcgagcgta aacgggaagg cgtatttggc aaattcaagc
    13621 ttggtcgatt cgtaggtgaa aagcagcatg atttctttta cctgactgca aagaaggcag
    13681 ttgctgcccg ctatttgttt ggcaatggtg agtttgctgt cctcaaaagt cttggattcg
    13741 atgctttgtt ttgcctgtat gaagtcggct tcggccatcg gccaggggca tccgtaagga
    13801 ccgttgtaac cgggcatgat atacaaattg gggtcataac catgctggtg ctggccgtcg
    13861 gaattgcggt taacctgctg agatcctgca ccctgattgt cgttgctgat ggttaccgta
    13921 ttcccgacac ccgtggcgga cacatttaca ttgttgttaa tggtcgtgtt attaataacg
    13981 gtagtcgtgg tattggtgcc ctgtgatgtg ccctgcggcg gataaccggt tacggtttgt
    14041 ccggcggatg gggccggtgc ctgcgccagc gggcaggtat tcatcatgcg cagcacgtaa
    14101 cggttctttt tccctttcct tacggagtag gtagtctcat tgccggcagt aacaaaaaca
    14161 gttttattga tttcacccaa ccgcttgttg aggaagatga tctttacttt ataactcgga
    14221 gccggcagac cggtgatttt gatgtatgtt tcgggcgagt cgttttgcaa cattccgttc
    14281 agcaccacgg aaaaaggttc gccgttttga gtaaaaaatg ccaggtcaga tgtctgggac
    14341 ctgacggctg ttgttaccag taaaatgatg agaatggtga gtatcctttt catagggcta
    14401 tggttaaatt gtaaggtttt cgggcgttac ccggatgagg aagaagtaac aacaatcagg
    14461 ctgcagttga atttaaaaat cttactttga actaccaccc gggcctttca accttgtaca
    14521 tcatggagga tatggcagaa atttttcgct ggatgtaagg tgtaggcctg tcggggcgcc
    14581 atttgcgggg gccgggcagt acagcggcga tagcagcgca ttcgctccgg gttagttttt
    14641 cggcaggctt atggaaatac tgttctgcag ccatttccgc gccatatatg ccatcgccca
    14701 tttctatcac gttcaggtac acttccatga tccgttcttt tccccacgta agttcaatga
    14761 gaccggtgaa gtaaacctca agccctttac gcacccagtt gcgtgccggc cataggaaaa
    14821 cattcttagc cgtttgttgt gaaatggtgc tggccccccg taattttcgc cctctcttgt
    14881 tcaacttctg ggctttttga atggctttga gatcaatccc gtggtgggtc ataaatttgt
    14941 tgtcctccga agcaataact gccaacggaa gatttttcgg catcttttca agcggtatcc
    15001 agttgtattg catcctgggg ctttcgcctt cggctaattg ctccaccacc cggatcatca
    15061 tcagaggggt ggccggcgga tttataaacc gaaatagtaa agcgacaacg atggttgaaa
    15121 tgagaaagaa aagaattgcg aagcgtaaat atttgacaat caatcgcatt attgagttcg
    15181 gattttgctc ttgctgaaag gactggttag tgtgaatcga aaaacaattt cttggcttcc
    15241 gctgcaaatg taccaattat ttggtaagtt tgttgctact atcaatgctg cttcaattaa
    15301 tctcaaataa ttgattttaa atgacttgag atagttgtta tatccgaata tttaaaattt
    15361 ctcctgttga aaaaatgagc ggaagaagga ggaaaatgcg gattgttcat gagttaacat
    15421 acagaaaaca aacaaaataa attcaaaacc atcgtatgac taaacttatc ggtataaggc
    15481 atgaagacaa atatgccatg gaacgccggg ttccgctcat tccgcggcat gttgagcgtc
    15541 tcatcaaaac taaaaaatta gacattgttg tgcagtcgtc tgacaaaagg atattcccgg
    15601 atgaggaata tcaaaaggcg ggagctgcca ttgccgacaa cctgaaaaac tgtgacgtga
    15661 tatttggtgt taaagaaatt cctgagacgt ttttcgaaac aggaaaaacc tatattttct
    15721 tctcgcatgt tatcaaagga cagtcctaca atatgccgat gcttcgcagg atgatggaat
    15781 tgaaatgcaa tctgatcgat tatgaaaagg tagtggatga gcagggtaag cggctgattt
    15841 tcttcggaag atatgccggg ctggccggaa tgataaattc cctttggtcg cttgggctaa
    15901 ggttgaaaga atatggaacg gacacgcctt ttcttaacct taaacaagcc cggcattact
    15961 catcgcttgc cgaggcccgt aacgccatat ctgcagtagg acaggagata gcagaaaaag
    16021 gccttccccg tgaattgcgt ccgttcactg tggcctttac cggttatgga aatgtttcgc
    16081 agggagcgca ggaaatttta gggcttcttc cggtgaagga aatcagtccg gaaaaactgc
    16141 tcgaactcga aaaacgggag catctgccca acaaccttat atacaaggta gtattcaaag
    16201 aggaacatct tgttgaaccg gttgagcccg gaacagaatt cgacctgcac gactattacg
    16261 ccaaccccca gcattaccgg ggtacattca gcaagtacct gccccatctc agcatgctga
    16321 taaattgtat ttattgggat gcccgctatc caaggcttgt taccaaagag caactggccg
    16381 gcttgttccg tgatggaagg cccaaactga cggtggttgg cgatatttcc tgcgacgtga
    16441 acggcgctgt tgaatttacc gaaaaagcca cggaaattga agaccctatc ttcgtttacg
    16501 atccttttat tcaatccatt accatgggcc atcagggcga aggaatgctg gtgatggccg
    16561 ttgatattct tcccagcgag ttgccgcgcg attcttcatc cggcttcagc gatgtgctga
    16621 tgaatttcgt gaaaccgatc gctgattgtg acttcaatga cgagttcgaa gaccttgatc
    16681 tgccgcgtgc cgtcaaaagg gcccttatcc tccacaacgg agaacttaca cctgactaca
    16741 ggtatctcga agaatatatc aatgcctgat aaatcataaa cactttcggc ggttccggac
    16801 ctgctccgga tatcgcagaa aacaactaat caacacaata tcattaataa tcaggttcct
    16861 ctaggaggaa caggtaaaaa ggaaaaatca atattatgaa gaaagtcctg attctgggcg
    16921 ctggtcttgt ggttcgcccg attgttaaac accttttaag caaaggtatt catgtaacgg
    16981 ttgctacccg caccaaatcg aaagccgaag aaatgattga cggccatccc aacggtacgg
    17041 ctgttgcctg gaccgtagaa gacgaagcca agcttagcca gatggtttcc gagcatgatc
    17101 ttaccgtcag cctgctgcct tatgtgcatc acttgctggt tgccaaacat tgcatcgcca
    17161 ataaaaagaa catggttacc accagctatg taaaacctga gatgcaggcg ctggatgctg
    17221 cagccaagga agcaaacatc atcatcctga atgaatcggg ccttgatcct ggtatcgacc
    17281 acatgtcggc tatgcgtatc atcgacaatg tgcacaaaaa aggcggcaaa atcgaagagt
    17341 tttacagttt gtgcggcgca ttgcctgctc ccgaactggc cgatgacaac ccattccggt
    17401 ataaattttc ctggagccct aagggcgtgg tgatggccgg caacaacgac gccatgtatc
    17461 tgaagcaagg caaagtggtg aaaattccga cacaggatct gtttaaaaat cccaaacagg
    17521 tagattttcc gaaagtggga aagctggagg tatatcccaa ccgcgattcc atcatgtata
    17581 aagaaatcta tggcattcct gaaactacca ccatcatgcg cggaacattc cgttacctgg
    17641 gatggtgcga aacgcttgac ctgctgaaga aactcaagct catttcgtat gataaggtga
    17701 acctcgaagg aaaaacgtat gcgcagtttg ttgccatgca gattggcgct gccgatgctt
    17761 ccgatatccg taagaaagtt gccgcttttg ccggtattga tgtcaacgct tatgccctca
    17821 atgctatgga atggcttggc tacttcagca atgaaccggt cggacgcgtc gaagattcac
    17881 cgttcgaagt taccagcgat atgatgatct cgaagatggg attaggtatg aatgagcgcg
    17941 atatggttgt tatgcagcat aatttcctgg cagtttaccc cgatggttcc aaggaaataa
    18001 tccgttcccg tatgctcgat ttcggaaccc ccgccaccga cacatccatt gcccgcacgg
    18061 tagcattgcc ggctgctatc gccgttgaaa tgatccttga aggcaaaatc cccgtgaagg
    18121 gtgttttcag gccggtact
//
LOCUS       DAOPYV010000064        18057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_3255_length_18057_cov_12.879291, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000064 DAOPYV010000000
VERSION     DAOPYV010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18057
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3255_length_18057_cov_12.879291"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PKI35_08855"
     CDS             <1..183
                     /locus_tag="PKI35_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44529.1"
                     /translation="AGIQSELSEAVSRSEASKANHNYFAYFIVRKEKKVFLLGSRHYK
                     RETLISHGNLLLLQVG"
     gene            complement(650..2101)
                     /gene="nrfA"
                     /locus_tag="PKI35_08860"
     CDS             complement(650..2101)
                     /gene="nrfA"
                     /locus_tag="PKI35_08860"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase"
                     /protein_id="HNX44530.1"
                     /translation="MLFIATAVIVFLLGMLAASITNRRAEKEYVYKPMIELKQGEPRN
                     AEWGKNFPREYQSYLQTADTSFRSKYNGSAAIDMLEEDPRLVILWAGYAFSKDYSQGR
                     GHYHAVDDIRNTLRTGAPMNGTPGPQPNTCWTCKSPDVVRLLGQMSPEEFYKGAWETR
                     GPEIVNPIGCADCHDPQTMNLVITRPALIEAFKRQGKDIAGATHQEMRSLVCAQCHVE
                     YYFDKHKTEGVSYLTFPWDKGFSADDIEKYYDSLNFSDWTHALSKAPMIKAQHPEYET
                     SQMGIHARRGVACADCHMPYKSEGGQKFTDHHIQSPLNNIANSCQVCHREEESELIAD
                     VYERQDKIIESRNKLEVLLVRAHVEAKKAWELGATDEEMKDILSDIRRAQWRWDFSAA
                     SHGASFHAPIEISRIIADGIVCAGNARTRLAGLLEQKGVRSEIPYPDISTKAKAQQYI
                     GLDMARLNAEKAEFKKVVIPEWIKQAAVRESKY"
     gene            complement(2152..2748)
                     /gene="nrfH"
                     /locus_tag="PKI35_08865"
     CDS             complement(2152..2748)
                     /gene="nrfH"
                     /locus_tag="PKI35_08865"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HNX44531.1"
                     /translation="MKGLLRNILPPPQWRLPVTILLGIFTGMGCYVFVISNAASYLSD
                     KPETCMNCHVMTPQYASWLHSSHRRVANCNDCHVPHNNVVNKYFFKAKDGLRHSLWFT
                     FRLEPQVIRIKSAGQKVVKDNCIRCHQPLLIDPEMQAVIKSDYYRNRVGRQCWECHRE
                     TPHGTVTSLSANPNAQVPTLSPVPSWLIKMMNEQKTKP"
     gene            2951..3436
                     /locus_tag="PKI35_08870"
     CDS             2951..3436
                     /locus_tag="PKI35_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44532.1"
                     /translation="MNLTHFHLLLNHLPILGSFFGILIFLSGYVFKELPVRKAGLGIF
                     IFAAIMAIPAYFTGKPAENAIKNLPDIAKGMIERHETVAVVALGCILALGLLSAVVFY
                     LITCKKGKTKILMTSILILSLVTFGLMAWTARTGGQIRHSEIRPADFPASLMISTEEN
                     E"
     gene            complement(3565..3999)
                     /locus_tag="PKI35_08875"
     CDS             complement(3565..3999)
                     /locus_tag="PKI35_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44533.1"
                     /translation="MKRILISLALIAGISLASYAQNAKSGAKVSIKSTTTESASVVTI
                     QKKEVKTVSSGIVASLKSSLNLTEKQKSKVSAAVTSFLKGKAKIAELATANQTEYSQK
                     LMSLTRKLNAKLKGTLSAEQFTKYNGLKSTTVSGSAMSQLFN"
     gene            4274..5356
                     /locus_tag="PKI35_08880"
     CDS             4274..5356
                     /locus_tag="PKI35_08880"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX44534.1"
                     /translation="METIAWLGFSQGLFAAILMFTKNERGVSDKILTGWLSLLAIEFL
                     TCGLDYRVFGHPILTSSFLLFNPAFYLYVQSLVNPAFSLKGIQLLHLFPYLFCRIFAY
                     LAQEPFELHAYLVPDTTLWFRIVFAIATVLSWIGYNSATALTVVKYRKNLENEFSTIE
                     SNLKIGWLFFIIVFYNLYCLALMIIGILILFKKLEVVPVHVFNYSILLALIYILSYYG
                     LRQRRLFQPAPPLKPADERYRYSSLSADKKEAIRTLLINYIEKEKPYLNPELNMNMLA
                     AALEVPKHQLTEVLNGDLGKNFFRFVNEYRVEAVKNKLKHNRRNYSVEAIGYDCGFNS
                     KSTFYTVFKQIAGITPAEFENSIKAK"
     gene            complement(5596..6591)
                     /locus_tag="PKI35_08885"
     CDS             complement(5596..6591)
                     /locus_tag="PKI35_08885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44535.1"
                     /translation="MKKITLFMLFLLVLGTTVSKGQIVLSSCTAPDSIVAKYTDDADR
                     LALGRFYRNALPYADSVSIPQAYSDTALKALLAVYNATELPERDTVVELYDIHTCMYA
                     LRRFAVIADSNQSWMQQLRNGVIPTGEPAVDSLMQLFNLSVESYSSVSYQGNHTVHFT
                     SDKNYNILALVGLWNSVPGFYSCLPHGFMCPSMSLKDSIYSDHVELTYSHGWGDCSAG
                     CLHRYWKFNVYFDCSVGFAGSYGVPVPIGMEDHFSNEVSVLPNPFSQGISVNGITPPF
                     LYSIRDMSGKVIISEECTGNEIENLDVLKPGFYILSVNDHGRIFRCKIIKAWPAL"
     gene            6883..>8054
                     /locus_tag="PKI35_08890"
     CDS             6883..>8054
                     /locus_tag="PKI35_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44536.1"
                     /translation="MPCKALTFYLVFGFALASHAQGLFESSLADSSATGPHEKSFDMN
                     GFVRGVAYGGSEYTDFSNLSGEFALKAGLSAGKTYFFGDARLREGIFFGRRKSEFELK
                     EAYAGYTGERFDLFLGNQIICWGRTDGFNPTNNLTPNDYFLLTPDPDDQKLGNFMLRP
                     KIRITGKTELEIVVIPVFKPSVYRYDLFDMGNASFARVLLPVAGLKNASLAGRFNVEL
                     PSAGFSVSYFHGYDPFYGFKVDTVSFRPEPFVDYSPQVYRKNTVGADFAVPVSQWIFR
                     GEAALNLTKNYREEMEIPNPDLSFVLGAERAIGGFTAIFQYIGKYTLDFTPLTKPVLA
                     DPYNPQAQMQYALDLIRYESKLFNRKIFNQQEETNHALFLSVSRSIAHEVLNAELSG"
     assembly_gap    8055..8154
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8211..10502
                     /locus_tag="PKI35_08895"
     CDS             8211..10502
                     /locus_tag="PKI35_08895"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX44537.1"
                     /translation="MNNDILSIRYRWLFIALAALITVLVAIPVPNSRINTDLNAYLPE
                     KMPSKVNLEKLESVFGKTDPVILIFETDDVLNGSTLKRIKAMNDSLEKMPQFRDVVSL
                     FTTKYIRSEEGSMVVDPVVKNFPANRAEREKLRAEIRDNPLAYKLVVSGDFRYTLIML
                     NPAQGVSDDELFKTINRLLKENPGPEKTYLNGMPYLRDEIQKKAIRDLAILMPLGLLI
                     MIGFLYFSFREIKGVLVPLSVVVMSIVLAMGLMPLLGYDLSLIAVLVPILMIAIANNY
                     GVHIITRYQELNARYRSRSMNTILDEALYYLSNPILLTALTTIVGILGMVTHIMLPAK
                     QMGIVSAAGITFALLLSLYYVPAVMSFLKTGKPNKSFIVEKHTAVDKFLTWSGKVATA
                     SPKRVIAVFAIVLLLTGAGIYRLKVSFNNENMMPASHPLQISTKIANDHLGGTKIISL
                     MFEGDIKDPVLLKTMDRFETELKKLPETGSVTSLATVIRTISRAMNDPGDPMYDKIPD
                     SRDAIAQYIEFYNLSGDPDDFEQLVDFDYTKAVMNIQFRATGIREFRKVESAIDKLMA
                     SAPYCRLKAGHCIVEKEMAEAIVKGQNYSLVFAFGAIILLLWWIFRSLKAGLMGSIPL
                     VFCIMCNFGLMGWLGFELDIATSLLSSVAIGIGVDYTIHLFWRLKTELSDGVEYHEAI
                     RKTLKTTGRGISINAFSVMTGFAVLLLSGLTILKAFGFLIIFSLLLCLLCAILLIPAI
                     CLQLRPAFLEKSPIQNFNRINGL"
     gene            10499..11272
                     /locus_tag="PKI35_08900"
     CDS             10499..11272
                     /locus_tag="PKI35_08900"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNX44538.1"
                     /translation="MKTNNLFMAAFIMLVPCRMEAQDARQIAEKASDIINPGSMEMTS
                     TLKIFDNKGNVRVREVTNATAKFGETTKTLIRFLSPADVKGTTILIYDYENKDDDMWI
                     YLPSLRKTRRIVSSEKGKSFMGSEFSNADMSKPTMDDFTYVLKGAETFNGKDCWKVES
                     KCKDAGVEQDNGYSRKIAWIDKGTCLTYKVEFYDANGILLKAETLSDYRKQSNGKYFA
                     FRMNMQNLQNNRKSEIVINKYQPGSALKEPAFSANNLGE"
     gene            11282..11710
                     /locus_tag="PKI35_08905"
     CDS             11282..11710
                     /locus_tag="PKI35_08905"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HNX44539.1"
                     /translation="MKPFIPASILALFIAASSFTQNLTISISGIRSRKGDICIGIYNN
                     SRQFNTDHPYRSLVVPKDKIKDGKIAVLIRDLPAGQYAIAFLDDENSNGKMDYRFMLP
                     AEGCGFSNYRHKGIKKPRFDDFDFELTQNNSYIRVEMQYY"
     gene            complement(11768..12331)
                     /locus_tag="PKI35_08910"
     CDS             complement(11768..12331)
                     /locus_tag="PKI35_08910"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNX44540.1"
                     /translation="MEKNFRIYTKTGDKGETSLIGGKRVPKYHSRIEAYGTLDELNSF
                     IGYLRDQLDHEPMKSLLLEVQDRLFVAESLLAADPDAAITKPLPALHDDDVAMLEREI
                     DRMNESLPPLSTFLLPGGHPLVSLSHVCRTVCRRAERLCIKLNTESPVDEIVIRYLNR
                     LSDFLFVLARYVAFLINVPDLPWNPET"
     gene            complement(12377..13105)
                     /locus_tag="PKI35_08915"
     CDS             complement(12377..13105)
                     /locus_tag="PKI35_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX44541.1"
                     /translation="MDGEVIRLVDITRNYKVGTEIVRALRSVSLTIRKNEFVALMGPS
                     GSGKSTLMNLLGCLDTPTSGQYFLNGKDVSKLNDLELAEIRNKEIGFVFQTFNLLPRI
                     TAIENVALPQVYAGITKAKRMERAREVIEEVGLTDRLHHKPNEMSGGQRQRVAIARAM
                     VNNPSIILADEPTGNLDSVTSHEIMALLEKIHKDGNTIVLVTHEEDIARHAHRIIRLM
                     DGQIADDYLNAEIYSSLKKATVTV"
     gene            complement(13196..13978)
                     /locus_tag="PKI35_08920"
     CDS             complement(13196..13978)
                     /locus_tag="PKI35_08920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX44542.1"
                     /translation="MTGYLKYFVKSKTKYKIHSPFVYSLITEVFEKKAETPEILAIRD
                     IYARLRRSRRVLEVTDFGAGKGKKSYILRFRSVGQIIRKSSVTPEPGGLLYKLAARFQ
                     PAVILELGTSLGISTLYLSKGAPGARVITIEGCSTTSEIARENFSEAGISNVEMHTGR
                     FEDILPGTLNDCGKVDFVFIDGHHEYGPTRMYVEMILGKARDETVIVIDDIHWSKGME
                     KAWEELCNFPAVTVSIDIYRFGILFLRKGLSKQHFIIRYRKP"
     gene            14089..14964
                     /locus_tag="PKI35_08925"
     CDS             14089..14964
                     /locus_tag="PKI35_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HNX44543.1"
                     /translation="MYFRKQMTKIGNYLSMVKFAHTIFAMPFALFGFFMAMEHPGHTS
                     DWLLLFFVILCMFFARNAAMSFNRWADREFDKKNPRTAIREIPAEIIRPESALAFCLL
                     NALFFIATTWFINRLCFYLSPVALLVILGYSFTKRFTYFSHFVLGLGLSLAPTGAFLA
                     VTGQFAWPPVILSAAVLCWVSGFDIIYALQDDDFDRENRLKSIPVQLGRQRALILSIL
                     LHAITAILLFILGIRNNYGFLYWTGYSVFIIMLAYQHFIVKADDLRRVNVAFFTLNGF
                     ASILYASFGIAGILI"
     gene            complement(14935..16050)
                     /gene="lpxK"
                     /locus_tag="PKI35_08930"
     CDS             complement(14935..16050)
                     /gene="lpxK"
                     /locus_tag="PKI35_08930"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNX44544.1"
                     /translation="MELIRILLFPLAVLYGLVARIRNAFFNWGIFRQVSFSLPVISVG
                     NLCAGGSGKTPMVEYLIRLLNQHYRIATLSRGYKRKTNGFLMADETSTSSQIGDEPLQ
                     YFRKFKNIMVAVDENRRRGIRSLLNMGNPPGVILLDDAFQHRRVKPGLSILVTDYFKT
                     YVHDQMLPVGRLREPIAGASRAQIIIVTKSPRIFSPILRRQITEELKPAPHQQLFFSY
                     VKYLGLEPVYAELPPFSLKEQRPSTIMMVTGIANPSPMEEYLRSACNELVMIPFPDHH
                     RFEPHDLAMIRQRFDELHSRLKIIVTTEKDAMRLRDGQSETILKNLPVYYLSMQHEFH
                     EEDKRKFDAFILDYLSGYQKEPGAPETGSDENPGNTE"
     gene            16159..17253
                     /locus_tag="PKI35_08935"
     CDS             16159..17253
                     /locus_tag="PKI35_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNX44545.1"
                     /translation="MRLKEIKKAFEDFAPLSLQESYDNSGLLIGDEEAEIQSALIALD
                     VTEAVLDEAISKGCNLVISHHPLIFGNLKTVTSGTSTGKMIRKAVQYDIAVYALHTNL
                     DNIAGGVNNILCDKLGIINRQVLKPLKGLLQKLVTFCPVEKAAEVRNAVFAAGAGFIG
                     HYDCCSFNAAGEGTFRAGEGTNPYVGETGALHFENEVRIETVFPSYLRSKVVSAMIRS
                     HPYEEVAYDIYNLDNTYAGAGAGMTGDLTADTDPREFLEKVKMTLGLGCIRHTPFTGK
                     KVRKVAVCGGAGSFLIPDALASGADIFLTGDIRYHDFFVPENSMLLADIGHYESEQFT
                     KELISTLLKKKFPTFAHFISETPTNPVNYL"
     gene            17282..>18057
                     /locus_tag="PKI35_08940"
     CDS             17282..>18057
                     /locus_tag="PKI35_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44546.1"
                     /translation="MAKSVAKSGGSSADATRKTPETPVTESMETANNTPVENELDELE
                     ISVEKKLVALHSLQQIDSQIDRIRIIRGELPLEVEDLEDEIAGLETRIDNHIQEIQSL
                     EKQIGDKQIAIRESQNLIKRYEEQQNNVRNNREYDALTKEIEFQSLEIQLAEKRIREF
                     NLQHDQYADEIEHAQSVLEDRRNDLEVKRSELSDIVTETENEEKELLRKSEAQQKFIE
                     DRLLLAYKRIRKNARNGLAVVTVERDACGGCFSAIPPQRQL"
BASE COUNT         4771 a         4383 c         4081 g         4722 t
ORIGIN      
        1 gcagggatac aatctgaact tagcgaagcg gtttcgcgaa gcgaagcaag taaagcaaat
       61 cacaactatt ttgcgtattt cattgtccgt aaagagaaga aggttttttt attaggatca
      121 cggcattata aaagagaaac tctgataagc cacggcaacc tcttgttatt gcaagttggg
      181 taatcccgta tttaagccaa tcagagtttg atacgaaatt accgaaaaca tcgacgccgg
      241 taattttact tgcctacatt gtatggcaaa acgattcaca aaattatatc ttaattactc
      301 taaatcaata tataatcaaa aaaaataaac cagagacagg attaaaactc aggcagaact
      361 ctggattggc tacaatattt tagtttaatt cgtcaccgga ccactgatgc aactgacagc
      421 aaaaagcaaa aatgtcggct caatcacgat tgtagaactg cccggcgttt tgctctcatt
      481 tgaaacttac gttgtattat ctatgtcaac cctgcctcgc gccagccaac aggtggcagg
      541 cagggataca atctgaactt aacggtctca cgaatcaaaa cgggtgggca aaccgatatt
      601 gggttacagc ttcaggctcc tgagatcatt ttatgatttt cattacctgt cagtacttgc
      661 tctcccttac agccgcctgt tttatccact ccggaataac aactttctta aattccgcct
      721 tttcggcatt gagcctggcc atatcgagcc cgatatattg ctgggctttg gctttggtgg
      781 agatatccgg ataaggaatt tcactcctga cgcctttttg ttccagcagt cctgccagcc
      841 gggtacgggc attgcctgca cagacaatgc catcggcaat gatccggctg atttcaatgg
      901 gagcatgaaa cgaagcgcca tggcttgccg ccgaaaaatc ccaccgccac tgcgccctgc
      961 ggatatcgct gagaatatcc ttcatctctt cgtcggtagc gccgagttcc catgcttttt
     1021 ttgcttccac atgggcacgg acaagcaata cttccagttt attgcggctc tcaataatct
     1081 tgtcctgcct ttcgtataca tcggcgatca gttccgattc ttcttcccgg tggcatacct
     1141 ggcaggaatt ggcgatattg ttgagcgggc tttggatatg atgatcggtg aacttctgcc
     1201 cgccttcgct tttgtaaggc atatggcaat cggcacaggc cactccgcgc cgggcatgga
     1261 tgcccatctg cgaggtttcg tattcaggat gctgcgcctt gatcatggga gctttgctta
     1321 acgcgtgggt ccagtcgctg aagttaaggc tgtcgtaata cttttcgata tcatcggcgc
     1381 taaagccttt gtcccatgga aaagtaagat aggagacccc ttcggtttta tgcttgtcga
     1441 aataatactc cacgtgacac tgcgcgcaaa ccagcgacct catttcctga tgcgtcgccc
     1501 cggcgatatc cttcccctgc cgtttaaaag cctcgatcag agcgggacgg gtaataacca
     1561 ggttcatggt ttggggatca tggcaatcgg cgcaaccgat agggttcacg atttcggggc
     1621 cccgggtctc ccaggcgcct ttgtagaatt cttctgggct catctgcccc aacagcctca
     1681 ccacatccgg gcttttacag gtccagcagg tattgggttg agggcccggt gttccgttca
     1741 tcggggcgcc ggtgcgaagg gtgttcctga tatcatcaac ggcatgataa tgccccctgc
     1801 cctggctgta atctttcgag aaggcatacc ccgcccacag gatcaccagc cgcggatctt
     1861 cttcaagcat atcgatcgcg gcgctgccgt tatatttgct gcgaaacgac gtatcggcag
     1921 tttgcaggta ggactggtat tcacggggaa aattcttacc ccattcggca ttccgcggct
     1981 cgccctgttt cagttcaatc ataggtttat agacatactc cttttcggca cggcggttgg
     2041 taatggatgc tgccagcatg cccagcagga aaacaatgac agccgttgca ataaaaagca
     2101 tccagtaaaa ccagggtttc ctttttttta tggtatccat gattgatggt tttatggttt
     2161 cgttttttgt tcattcatca tttttatcag ccacgaaggc accggactaa gcgtcgggac
     2221 ctgtgcgttg ggatttgccg aaaggctggt tacggtgccg tgcggcgttt cgcggtggca
     2281 ttcccagcac tgacggccaa ccctgttcct gtaataatcg gactttatca ccgcctgcat
     2341 ttcaggatcg atgagtaatg gctggtggca acggatgcaa ttgtctttga cgaccttctg
     2401 tccggcggat ttaatccgga tcacctgcgg ttcgaggcga aaggtaaacc agagggaatg
     2461 acgcagcccg tcctttgcct tgaaaaagta tttattgacc acattgttgt gaggcacatg
     2521 gcaatcgtta caatttgcca cccggcggtg cgaactgtga agccatgaag catattgcgg
     2581 cgtcatgaca tggcagttca tacaggtttc cggtttatca ctcaggtaag aagcagcatt
     2641 ggaaatgaca aaaacatagc aacccatgcc ggtgaagatt cccagcagga tagttaccgg
     2701 caggcgccac tgcggaggag gcaaaatatt ccgcaacaat cctttcatgg tggcgaatca
     2761 gtttaaaaat tcacggaatt gctgattttc tcaggtaaat ataataagaa tacacttgat
     2821 tttataaaga gacataaatt aattttccga tgttccggaa ttatttaaaa ggcgctgctc
     2881 ttcttttacg attgccgcta ttggttttaa cttcatttca tacatttgaa gcgcaataat
     2941 atcaagctcc atgaacctca ctcattttca tctgctgctt aaccaccttc cgatccttgg
     3001 atcctttttc ggcatcctga tctttctttc cggttatgtg ttcaaagaac taccggtcag
     3061 gaaagcggga ttaggaatct ttatcttcgc cgcaatcatg gccattccag cctattttac
     3121 aggaaaaccg gcggaaaatg caatcaaaaa ccttccggat atagcaaagg gcatgatcga
     3181 acgtcatgaa acggttgctg tcgtggcgct ggggtgcatc ctcgcgctgg ggctgttgtc
     3241 agccgtcgta ttttacctga taacctgtaa aaagggtaaa acaaaaatcc tgatgacgag
     3301 catcctaatc ctctccttag taacctttgg cctgatggcc tggaccgcca ggacaggagg
     3361 ccagatcagg cattccgaaa tcaggccggc ggatttccct gcatcgctca tgatcagtac
     3421 ggaagaaaat gaataaaaat gaaaaccagg caacctctcc tccgaaagta acgtttaaaa
     3481 aaattaacaa aacactgatt gcctgaacaa aaaaaaagcg cgcagttgac taaaccgcgc
     3541 actttaagta atctcaaccg gccattagtt gaacagttgc gacatagcgc tgcccgatac
     3601 cgtcgttgat ttcagcccgt tgtatttcgt aaactgttca gcgctcagag tacctttcag
     3661 tttggcgtta agcttgcgag tgagggacat aagcttttgc gagtattcag tttgattagc
     3721 cgtcgccagt tccgcaattt ttgcttttcc cttcaggaag ctggtcacgg cagcgctgac
     3781 tttcgatttt tgcttctccg tcagattcag cgatgatttt agcgaagcga caatcccgga
     3841 tgataccgtt ttgacctctt tcttttggat ggtgacaaca gaagctgatt cagtcgttgt
     3901 cgactttatg ctgactttgg ctcccgattt ggcattctgc gcataggaag ccagagaaat
     3961 tccggcaatt aatgccagtg aaattaatat tcttttcatt tgtttgattt taacttatca
     4021 aagttactca ctcccggaag ccgttttgca gccaataccg ttaaaagttg ttaataacgg
     4081 tcatccctga aaaaagccgg atatcgttgt aatatttttc agaaatgcat atttttacag
     4141 agaatcagca gaagcaaact caccggcaag aaggggcttc agaattcccg ggacccaaat
     4201 gcatggctac gattcactga ttttttcagg taaatttgta atccacagga cgataaaaac
     4261 ttagccggaa aacatggaaa ccatagcatg gcttggattt tcgcaagggt tgtttgcagc
     4321 catccttatg ttcaccaaaa atgagcgagg cgtttcggac aaaatcctga ccggctggct
     4381 gtcgttgctg gccattgaat tccttacctg cggtctcgat tatcgggtgt ttggtcatcc
     4441 catcctcacc agttctttcc tgcttttcaa tccggcattt tacctttatg tccagtcgct
     4501 ggtaaacccg gctttcagtc tgaaagggat acaactgctc catcttttcc cgtatctgtt
     4561 ttgcagaatt ttcgcctacc tggcccagga acctttcgaa ttacatgcct acctggtccc
     4621 ggacactacg ctttggttcc gtattgtttt tgccattgcc acggtcttat cctggatcgg
     4681 gtacaattcg gcgaccgccc ttacagtggt aaaatatcgt aaaaaccttg aaaacgaatt
     4741 ttcgaccatc gaaagcaacc tgaaaatcgg ctggcttttt ttcatcatcg tattctacaa
     4801 tctttattgc cttgccctga tgatcatcgg aatccttatc ctttttaaaa agcttgaagt
     4861 cgttccggtt catgttttca attattctat cctgctggcg ctcatataca ttctcagcta
     4921 ttacggatta aggcagcgac ggttattcca gcctgctccg cccctgaaac ccgccgatga
     4981 gcgttacaga tacagcagcc tgtcagcaga taaaaaagaa gctattagga cattgctgat
     5041 caactacatt gaaaaggaaa aaccgtacct gaacccggaa ttaaatatga acatgcttgc
     5101 ggcagcgctg gaagtgccca aacatcagtt aactgaggtt cttaacggcg acctggggaa
     5161 aaatttcttc cgtttcgtga acgaataccg cgtggaagcc gtgaaaaaca aactgaaaca
     5221 caacagacgt aactacagcg tggaagccat cggctacgat tgcggtttca acagcaaatc
     5281 gacattttac accgtcttta agcagattgc cggaataacg ccggccgaat ttgaaaattc
     5341 gattaaggct aaatgagctg cccaatattt acgctgatca tggcatacca ggaagcgaca
     5401 ttgtcgacag gcttatatca tcaacacgtc gggacaacaa aacctgctga aaggcagaat
     5461 tatgatgctc aagcagaatg agattttttc atccggtttg aaattttgca atcgacagta
     5521 caaatttctg gtaataatca gaacagaata aatcttttat ctgatttatc ttagcgttca
     5581 gtgattgccg gcgatttaaa gggcaggcca ggctttgatg attttacacc tgaaaatcct
     5641 tccatgatca ttaaccgaca gaatataaaa tcccggtttt agaacatcca gattttctat
     5701 ctcattcccg gtgcattctt ctgaaattat caccttgcct gacatatcac ggattgaata
     5761 caagaaagga ggcgtaattc cattgacaga tattccctgg ctgaaaggat tcggcaatac
     5821 agaaacctca ttgctgaaat gatcctccat tccaatcgga accggaacgc catagctgcc
     5881 tgcaaaccca acgctgcaat cgaagtaaac attgaatttc cagtaacgat gcaggcaacc
     5941 agccgaacaa tcgccccatc catgagaata ggtcagttca acatgatcgg aatatattga
     6001 atcctttaaa ctcattgaag ggcacatgaa accatgtgga agacaggaat aaaatccggg
     6061 aacggaattc cacaaaccaa ctaaagccag gatattataa ttcttatcac tagtgaaatg
     6121 aacggtatgg ttaccctgat aactcactga actataactt tctacgctaa gattaaataa
     6181 ttgcatcagg ctatctaccg ccggttcgcc tgtcgggata actccatttc tcaactgctg
     6241 catccatgat tgatttgaat cggcaatcac ggcaaatctc cttaaagcat acatacaggt
     6301 atgaatgtca tataactcaa ccaccgtatc tctttcaggt aattcagttg cattataaac
     6361 ggcgagcaaa gctttcagcg ccgtatcgga ataggcctga gggattgaaa cactgtcggc
     6421 gtaaggcaaa gcgttcctgt aaaaccttcc taaggcaagc ctgtcggcat catcggtata
     6481 ttttgccaca atactgtcag gggcagtgca ggacgataaa acaatctgtc ctttggaaac
     6541 cgttgttccc aatacaagca ggaacagcat aaaaagagta atcttcttca tattaaattg
     6601 attttaaaat aattgaacaa tataaatttc tcttaccagg gcctataaac aattccgatt
     6661 atggctttat gaagatacaa atataatgtg taaaatcaat ataattaaaa aatggccgca
     6721 tttattaaaa cggttttcac cagttcttta tcatatttat agtccggatt ttaaattttc
     6781 aaaatccgaa ccacaattag cactccgtct tttaaaaccg ggacaggatg ccttccggcc
     6841 atgggtaagt ttgtatgatt tttcatccca aacaggtctc ctatgccgtg taaagcgctc
     6901 actttctatc ttgttttcgg attcgccctg gccagccatg cacagggttt gttcgaaagt
     6961 tcgctggccg actcttcagc gaccggtcct catgagaaat cattcgatat gaatggtttc
     7021 gtaaggggcg tagcgtacgg cggctccgaa tatacagatt tctcaaacct ttccggggaa
     7081 tttgccctga aagccggatt atcggctgga aagacttact tcttcggcga cgcacgcctt
     7141 cgtgaaggta tttttttcgg caggcgcaag tcggaatttg agctgaaaga agcgtatgcc
     7201 ggatataccg gtgaaaggtt cgatctcttc cttgggaacc agatcatctg ttggggacgc
     7261 accgacggct tcaacccgac caacaacctg acgcccaacg actatttcct cctcacgccc
     7321 gatcctgacg accagaagct gggcaatttt atgctgaggc caaagatcag gattaccggc
     7381 aaaacagaac tggaaattgt cgtgataccg gtctttaaac catctgttta ccgttatgac
     7441 ctgtttgaca tgggcaatgc ctcctttgcc cgggtccttt tgccggttgc cggcttaaaa
     7501 aatgcaagcc tggccgggcg tttcaacgtt gaattaccat cggcaggatt ttcggtttca
     7561 tacttccacg gttacgaccc tttttatggc tttaaggttg ataccgtctc attccggcct
     7621 gaaccctttg ttgattacag ccctcaggtt taccggaaaa acaccgtcgg cgccgatttt
     7681 gccgttcctg tttctcagtg gatattcagg ggtgaagccg ccctgaacct taccaaaaat
     7741 tacagggagg aaatggagat tcccaatccc gatctttcct tcgttcttgg agcggaacgc
     7801 gccatcggag gctttaccgc catttttcag tacatcggga agtacaccct tgattttacc
     7861 ccgcttacca aaccggtcct ggccgatcct tacaacccgc aggctcagat gcagtatgca
     7921 ctggatctga tacgatacga atcgaaactc ttcaaccgga aaattttcaa ccagcaggaa
     7981 gaaaccaatc acgcgctgtt tttgtcggtc agcaggtcca ttgcccatga agtgctgaat
     8041 gcagaactgt cgggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccggaa
     8161 ctttcggacc tgccttctga cgctgccggg caagccttca aacaacagcc atgaataacg
     8221 atatcctgtc tatccgctat cgctggttat tcatcgcgct cgccgcgctg ataaccgttc
     8281 ttgtggcgat accggttcca aattcaagga taaacaccga cctgaacgcc tacctgcccg
     8341 aaaaaatgcc ttcgaaggtt aaccttgaaa agcttgaaag cgtcttcggt aagaccgatc
     8401 ccgtcatact gatttttgaa acggatgatg tactgaatgg gtccacactg aagagaatca
     8461 aagccatgaa cgattccctg gaaaaaatgc cgcagttcag ggatgtggtt tcacttttta
     8521 caacgaaata catccggtcg gaagagggca gcatggtagt tgatccggta gtaaaaaact
     8581 ttcccgctaa ccgggccgaa cgtgaaaagc tgcgtgccga aatcagggat aatccgttgg
     8641 cttataagct tgtagtatcc ggcgatttca gatacacgct gatcatgctt aatccggcgc
     8701 agggcgtttc ggatgatgaa ctctttaaaa ccatcaacag gttattaaaa gaaaacccgg
     8761 gaccggaaaa gacctacctc aacgggatgc cttacctgcg cgacgagatc cagaaaaaag
     8821 ccattcgcga cctggccatc cttatgccgc tgggattgct cattatgatc gggtttctgt
     8881 acttttcatt ccgtgaaatc aaaggcgtcc ttgtaccgct ctcagtagtt gtaatgtcca
     8941 ttgtgctggc catggggctg atgcccctgc tgggatatga cctgagcctc attgccgtcc
     9001 tggtccccat tctgatgatt gccatcgcca acaactacgg cgtgcatatc ataacccgct
     9061 atcaggaatt aaatgcacgt tatcgctccc gttccatgaa caccatactc gacgaagcat
     9121 tgtactacct cagcaatccg attctgctta ccgcgctgac aaccatcgtg ggaatcctgg
     9181 gcatggtgac acatatcatg ctgccggcca aacaaatggg cattgtaagc gccgccggta
     9241 tcaccttcgc cctgttgctc agtttgtact acgttcctgc ggtaatgtcg tttctaaaaa
     9301 caggcaaacc aaacaagagt tttatcgtgg aaaagcacac cgctgtcgac aaattcctga
     9361 cgtggtcggg caaggtcgcc actgcttcgc ctaaaagagt catcgcggtc tttgccatcg
     9421 tgttgcttct gaccggcgcc ggtatatacc ggctgaaggt cagcttcaat aacgagaata
     9481 tgatgccggc ttcgcatcct ttacagatct ccaccaaaat tgccaacgat catttgggcg
     9541 ggacaaagat tatttcgctg atgtttgaag gtgatatcaa ggacccggtt ttactgaaaa
     9601 ccatggaccg ttttgaaaca gaattgaaaa aactgcccga aacaggaagc gtaacatctc
     9661 tggcaacggt gatccggacg atcagccggg caatgaatga tcccggagac ccgatgtacg
     9721 ataagattcc cgacagccgc gatgccattg cccaatacat cgaattctac aacctgagcg
     9781 gcgatccgga tgatttcgaa caactggttg attttgacta caccaaagcg gtgatgaata
     9841 tacaattcag ggcaaccggc atcagggaat tcagaaaagt ggaaagcgcc atcgacaaac
     9901 tgatggcctc tgctccctat tgcaggttga aagccggaca ctgcatcgtg gaaaaggaga
     9961 tggccgaagc catcgtgaaa gggcagaact actcgctggt attcgcgttc ggggccatta
    10021 tccttctcct ctggtggatc ttcaggtcgc tgaaggccgg cctgatgggt tccataccgc
    10081 tggtgttctg catcatgtgc aatttcgggt taatgggatg gctgggtttt gaactcgata
    10141 ttgctacttc gcttctttct tctgttgcta tcggcatcgg ggtagactac accattcacc
    10201 tgttctggcg tctgaaaacg gaattgtcgg atggcgttga ataccatgaa gccattcgta
    10261 aaaccctgaa aaccaccggg cgaggcatat ccatcaatgc attttcggtg atgaccggat
    10321 ttgccgtcct tttgctttca gggcttacaa tcctgaaagc tttcgggttt ctcatcatct
    10381 tttccctgct gctttgcctg ttatgcgcta tactgctgat tcctgccatc tgtctgcaat
    10441 tgcggccggc atttctcgaa aaatcaccca tacagaattt taatcgcatc aacggactat
    10501 gaaaacaaac aatttgttca tggcggcttt catcatgctg gttccctgcc ggatggaagc
    10561 ccaggatgcc cgccagatag ccgaaaaagc atcggatatc atcaacccgg gatcaatgga
    10621 aatgacctcc acgctgaaaa tttttgacaa caaaggcaac gtgcgggtac gtgaagttac
    10681 caatgcaacg gcgaaattcg gcgaaacgac aaaaacactg atcagattcc tctcccctgc
    10741 cgatgtcaaa ggaacgacca tccttatcta cgattacgaa aataaggacg acgatatgtg
    10801 gatttatttg ccgtcgctca ggaagacaag gcggatcgtg agcagcgaga aaggaaaaag
    10861 ttttatgggc tcggaattca gcaatgccga tatgagcaaa cccactatgg acgattttac
    10921 gtatgttttg aaaggcgctg aaacgtttaa cggtaaagat tgctggaaag tggagtccaa
    10981 atgtaaagac gccggtgtgg aacaagacaa tggctacagc cggaagatcg cctggattga
    11041 taagggtacc tgcctcacgt ataaagtgga gttttacgac gcaaacggca ttctcctcaa
    11101 agcggaaaca ctctccgact accggaaaca aagtaacggc aagtatttcg ccttccggat
    11161 gaacatgcag aatttgcaga acaaccgcaa gtctgaaatt gtgatcaata aataccagcc
    11221 aggatcagca ctgaaggaac cggcattttc ggcgaataac cttggcgaat aaagcgcaac
    11281 catgaaacct ttcattccgg cttccattct ggctttattt atcgcagcgt cttcgtttac
    11341 ccaaaacctg acgatcagca tttcaggcat ccgtagccgg aaaggagata tctgtatcgg
    11401 catttacaac aacagccgcc agttcaatac cgatcatccg tatcgaagtc tggtggtgcc
    11461 caaggacaag ataaaagacg ggaaaattgc cgttctcatc agggacctgc cggcgggaca
    11521 gtatgccatt gcttttcttg atgatgaaaa cagcaacggg aagatggatt accgatttat
    11581 gctgcctgcc gaaggatgcg ggttctcaaa ctacaggcat aaaggaataa aaaagccaag
    11641 gttcgatgac tttgattttg aactaaccca aaacaatagc tatatcaggg ttgaaatgca
    11701 gtattactaa gtcctcacgg aaacccaaaa ccggcagggc ctgaaaagtt tcaggccggc
    11761 attttattta cgtctccgga ttccacggca aatcgggaac atttattaaa aaagcaacat
    11821 atcttgccag gacaaaaaga aagtctgaaa gacggttgag gtaacggatc acgatttcat
    11881 caacgggaga ttcggtattc aatttaatgc acaaccgttc cgctctccgg caaacggtac
    11941 ggcagacgtg gctcagcgat accagcggat gtccgcccgg cagcagaaaa gttgacaatg
    12001 gcggcaacga ttcgttcatc ctgtcgattt cccgttccag catcgccaca tcgtcgtcat
    12061 gcaaagcggg cagcggcttt gtgatggcgg catccggatc agcagcaagc agcgattcgg
    12121 cgacgaaaag cctgtcctgc acttcgagca aaagggattt catcggttca tgatcgagct
    12181 ggtcgcggag gtacccgatg aaagaattta attcatcgag cgtaccgtat gcttcgatgc
    12241 ggctgtgata ctttgggacc cgtttcccgc cgatcagcga agtttcgcct ttatcgcctg
    12301 ttttggtata tatcctgaaa tttttctcca tgaacggcag gtatgaaaat gatgtcatga
    12361 atgtgcccgg gcggcgctaa actgtcaccg tggctttttt cagcgaactg taaatttcgg
    12421 cattcagata atcatcggca atctgtccgt ccatcaggcg gataatccgg tgggcatggc
    12481 gggcaatgtc ttcttcatgg gtaaccagga caatggtatt tccgtcctta tgaattttct
    12541 ccagcagggc cataatctca tggcttgtca ccgaatcgag gtttccggta ggttcatcag
    12601 ccagaatgat actcgggtta ttaaccatag ccctggcaat agctacgcgc tggcgttgcc
    12661 cgcccgacat ttcattgggt ttgtgatgca ggcggtcggt aagccccacc tcttcaatga
    12721 cttcgcgggc gcgttccatc cttttcgcct tagtaatgcc ggcataaacc tggggcagcg
    12781 ccacgttttc gatggccgta atcctgggca acaggttgaa tgtctgaaac acgaatccga
    12841 tctccttgtt cctgatttcc gccaattcaa ggtcgttgag cttgctgaca tccttcccgt
    12901 taaggaaata ctggccgctg gtgggtgtat ccagacaacc cagcaggttc atcaatgttg
    12961 actttcctga acccgacgga cccatgagcg caacgaattc atttttcctt atcgtcagcg
    13021 agaccgagcg aagcgccctt actatctcgg tgccaacctt ataattgcgg gtaatgtcaa
    13081 caagacgaat tacttcacca tccataagca taagttttgc atttactgtt cacaaaaata
    13141 atccgaataa gcatgcgaaa catatacact gttgatttct ggcgttcatc atttttcagg
    13201 gtttccggta ccggatgatg aaatgctgtt ttgataagcc tttccggaga aacaggattc
    13261 caaaacggta aatatcgatg gaaaccgtta cagccggaaa attgcagagt tcctcccagg
    13321 ccttttccat gcctttcgac cagtgaatat catctatcac aatcacggtt tcatcccggg
    13381 ctttcccaag gatcatttca acatacatcc tggtaggccc gtattcatga tggccgtcga
    13441 taaagacaaa atccaccttc ccgcaatcat tcagcgtccc cggcagaata tcttcaaaac
    13501 gtccggtatg catttcaaca ttcgaaatgc cggcttcgga aaagttttca cgcgctattt
    13561 ccgaagtagt gctgcatcct tcaatggtta tcaccctggc tccgggtgcg ccttttgata
    13621 gatacaaagt gcttataccc agcgatgttc caagttcaag aatgacggca ggctgaaacc
    13681 gggctgccag cttgtacagt agtcctccgg gctcgggcgt caccgatgat ttcctgatta
    13741 tctgcccgac gcttctgaaa cgcaggatgt aggatttttt gcctttaccg gcgccgaaat
    13801 cggtaacttc gagaacgcgg cggctccgcc ttaaccgggc gtatatatca cgaattgcta
    13861 atatttcagg agtttcagct tttttttcaa aaacttcggt tataagtgaa taaacgaacg
    13921 gggaatgtat cttatacttt gttttagact tgacaaaata ctttaaatac ccggtcacta
    13981 acctcaaaca cctgatcatg gtctgaaaat tttattgatt tccttgctgc aagataaaca
    14041 ggcagccatt ccgttcgcta tttttgccga caatgtaaaa caaacggcat gtactttcgt
    14101 aaacaaatga caaaaatagg gaactatctg tcgatggtta aatttgccca taccattttt
    14161 gccatgcctt ttgcgctctt tggttttttc atggccatgg aacatcccgg tcacacatcc
    14221 gactggctgc ttttattctt tgttatttta tgtatgtttt ttgcccgtaa cgcggccatg
    14281 agttttaacc ggtgggctga ccgtgagttt gataaaaaaa acccccggac tgccatccgt
    14341 gaaattcctg ccgaaatcat caggcctgaa tcggccctgg ccttttgcct gctcaacgca
    14401 ctgtttttca tcgccaccac atggtttatc aaccggctct gtttttacct ttcaccggtt
    14461 gcgctgctgg taattctcgg ttacagtttc accaaacggt ttacttattt ctcacacttc
    14521 gttttgggcc ttggcctttc cctggccccg acgggagcat tcctggccgt taccgggcaa
    14581 tttgcctggc cgcctgtaat actctcggct gcagtccttt gctgggtttc aggatttgat
    14641 attatttatg cgctccagga cgatgatttc gaccgcgaga accggttgaa atcgattccc
    14701 gttcagttgg ggagacaaag ggcgctgata ctttcgatcc tgttacacgc catcacggcc
    14761 atcctgcttt ttattctcgg cattcgcaac aactatggat ttctttactg gaccggttat
    14821 tcagttttca ttatcatgct tgcataccag cacttcatcg tcaaagcaga cgatctgagg
    14881 agagtgaatg tggctttctt tacgctcaac gggtttgcca gcatcctcta tgcttcattc
    14941 ggtattgccg ggattctcat ctgatccggt ttcgggcgcc cctggttctt tttgataacc
    15001 cgacaggtaa tcaaggataa aggcgtcaaa ttttctttta tcctcctcat gaaactcatg
    15061 ctgcatgcta agataataga ccggaaggtt ttttaatatg gtttccgatt ggccgtcccg
    15121 caaccgcatg gcatcttttt cggtagtaac aatgatcttc agcctcgaat gcagttcatc
    15181 gaaccgctgc cggatcatgg caagatcgtg gggctcaaag cgatgatgat ccggaaacgg
    15241 gatcattacc agttcgttgc aggccgaccg gaggtattcc tccataggcg aagggttcgc
    15301 aatacctgtt accatcatga tggtcgaagg ccgttgttct ttcagcgaaa acggtggcaa
    15361 ctcagcatat accggttcaa gtccgagata cttcacgtag gaaaagaaca attgctgatg
    15421 gggggccggc ttaagctctt cggtaatctg tctgcgcaag atgggtgaga atatgcgggg
    15481 cgatttggtg acaattataa tctgcgccct tgaagcgccg gcgatcggtt cacgtaatct
    15541 ccccaccggc aacatctggt catggacgta ggttttaaag tagtccgtca ctaaaattga
    15601 taagccgggc tttacccggc gatgctgaaa agcatcatcc agcagtataa ccccgggagg
    15661 atttcccata ttcaacaggc tgcgtattcc tcttctcctg ttttcatcta cggcaaccat
    15721 gatattttta aatttccgga aatactgcag cggttcatcc ccgatctgcg aacttgtact
    15781 tgtctcatca gccatcagaa agccattggt tttccgtttg tagccccggc tcagcgtagc
    15841 gatacgataa tgctgattca gcaaccttat aaggtattcc accatgggag tcttgccgct
    15901 tccgccggca cagagatttc cgacggaaat cacgggaagg ctgaaagaaa cctgtctgaa
    15961 gatgccccag ttgaagaatg cattgcggat acgggcaacc agcccataca ggaccgccag
    16021 aggaaatagt aatatcctga tcaattccat gatttaaaat tacaaaatta tacaactgat
    16081 gccctgttgt atggtatatt tgctgaaaat gtgagcgctc acgccctaat ctgtaaatta
    16141 gaacccaaac tttagatgat acgtctgaaa gaaatcaaga aggcttttga agactttgcc
    16201 ccattatcgt tgcaggagag ttatgataat tcgggtctgt tgataggcga tgaggaagct
    16261 gagattcaat cggcgctcat tgcactcgat gttaccgaag ccgtgctcga cgaagccatc
    16321 agcaaaggct gcaaccttgt aatttcccat cacccgttga ttttcgggaa cctaaaaaca
    16381 gtaacatcgg gcacatccac gggcaaaatg atacgaaaag ccgtccagta cgacatagcg
    16441 gtttatgccc tgcatacaaa ccttgacaat attgccggag gagtaaacaa tatcctttgc
    16501 gacaaactgg gaatcatcaa caggcaggta ttgaaaccgc tgaaaggttt gctgcaaaag
    16561 ttggtgacct tttgtccggt tgagaaagcg gcggaagtac ggaatgctgt tttcgcagcc
    16621 ggtgccggtt ttatcggaca ttacgattgt tgcagcttca atgccgccgg agaaggaact
    16681 ttcagggccg gtgaaggaac aaacccttat gtgggagaaa ccggagcgtt gcattttgag
    16741 aatgaagtgc gtatcgaaac ggttttccct tcctatctcc ggtcaaaggt agtttcagcc
    16801 atgataaggt ctcatcctta cgaagaagtt gcctatgata tttataacct tgacaacact
    16861 tatgccggcg cgggagcagg catgacgggc gatctgactg ctgataccga cccgcgtgag
    16921 tttctggaaa aagtaaaaat gacgctgggc ctgggttgta tccggcacac cccattcacc
    16981 ggaaaaaagg tcaggaaagt tgccgtttgt ggcggagcag gaagttttct gatacctgat
    17041 gcgctggctt ccggcgccga cattttcctt accggcgaca tccgttacca tgactttttc
    17101 gtgccggaaa actctatgtt gctggccgat atcgggcact atgaaagtga acaattcaca
    17161 aaagaactga taagcaccct gttaaagaaa aaatttccta cctttgcaca ctttatttcg
    17221 gaaaccccga caaatcctgt taactacctg taattaaaca tataacactc gcaatatagc
    17281 tatggcaaaa tcagttgcta aatcaggcgg atcatcagcc gatgctaccc ggaagacacc
    17341 tgaaaccccg gtaaccgaat caatggaaac agctaacaac acgcccgttg agaacgaact
    17401 ggatgagttg gaaatttcag tggagaaaaa attggttgcg cttcacagct tgcagcaaat
    17461 tgattcccag atagaccgca tccgcatcat caggggggaa ttgccactcg aggttgaaga
    17521 cctggaagat gaaattgccg gcctcgaaac ccgtatcgac aatcacatcc aggaaattca
    17581 gtcgctcgaa aaacagattg gcgacaaaca gattgccatc cgtgaaagtc agaacctcat
    17641 caaaagatat gaggaacagc agaataatgt ccgcaacaac cgcgagtacg acgctctgac
    17701 caaagaaatt gaatttcaat ccctcgaaat ccaactcgcc gaaaagcgca tccgtgaatt
    17761 taacctgcag catgatcaat atgctgatga gattgaacat gcacagtcag ttttggaaga
    17821 ccgccgcaac gaccttgaag taaaacgcag cgaattatcc gacatcgtta ccgagaccga
    17881 aaacgaagag aaagaactcc ttcgcaaatc ggaagcacag caaaaattta ttgaagaccg
    17941 tctcctgctt gcatacaaac gtatccggaa aaatgcacgt aacggcctgg ctgtcgtaac
    18001 cgtagaacgc gacgcctgcg gcggttgttt cagcgctatc ccccctcagc gccagct
//
LOCUS       DAOPYV010000065        17243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_3496_length_17243_cov_10.698801, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000065 DAOPYV010000000
VERSION     DAOPYV010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17243
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3496_length_17243_cov_10.698801"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /gene="ispF"
                     /locus_tag="PKI35_08945"
     CDS             complement(<1..409)
                     /gene="ispF"
                     /locus_tag="PKI35_08945"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNX44547.1"
                     /translation="MNQRIGFGYDVHRLVAGREFWLGGVRIPSETGADGHSDADVLLH
                     AVCDALLGAAALGDIGKHFPDTDPSLKDIDSKILLRRVSDMLNSCGYSVVNIDSTIAL
                     QAPKVAPYIDTMRRIIADILGIPVADFLKNTATT"
     gene            complement(507..1673)
                     /gene="porV"
                     /locus_tag="PKI35_08950"
     CDS             complement(507..1673)
                     /gene="porV"
                     /locus_tag="PKI35_08950"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HNX44548.1"
                     /translation="MLNKKCVFFFTLLAAMISATMSMAQGLNTVQSAVPFLMVSPDAR
                     SGSLGDAGVASTPDENSMHWNPAKYAFIDKQMGVAVNYSPWLHNLVPDISLAYLSGYS
                     RLDKNQTIAASLRYFSLGNIQFTSQFGEPLGDYKPNEFALDFAYSRKFTKTLSGSVAA
                     RFIHSNLTLRQDVGSQSTKPGNSVAADVSVYQQLPLQFSSTEGLFAWGVNISNIGAKI
                     SYSNDNIRKDFIPTNLRIGSSLTLDLDEYNRMSFMLDFNKLLVPTTPEYSDTNSTVIV
                     RGMDPDVSTAVGMLHSFYDAPDGFSEELREINISTGVEYWYNQQFAVRGGFFYEDPTK
                     GDRTYVTLGAGLRYNVFGLDVSYLVPLATDNPLENTLRFSLLLNFEAVSGGGKK"
     gene            complement(1776..5600)
                     /gene="porU"
                     /locus_tag="PKI35_08955"
     CDS             complement(1776..5600)
                     /gene="porU"
                     /locus_tag="PKI35_08955"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNX44549.1"
                     /translation="MNRSACILVFMFLLPLLAGANVTRHAVRLQWQAVTNIGNESSEP
                     LNVLYFDDAANLAGKGYAPFFFKVFRFNNDEVPVRVSIENTVYERVEPSELKQLTFQP
                     DVSDSISLEYNFWTEREKPVMTVSVSTLRLQGSAPEKLVSFDLIIETEPVTETALVKS
                     SAGSINSVLATGNWYKIRLDKTGVYSLTYDEIKAMGVNMAEVTPDNIRLFGNGGGMLP
                     ERNNKPRASDLLENAIQVVTSQPGTFKSGDYICFFATGPDKVNYDQLTKRFDHEKNDY
                     SDYSCYFLTFGGNGKRIDDIAQTTLPPSEIVTRYTDFMFYENDLYNLIKSGRYWVGER
                     MDMSSNQVNLPAYTFPDFDSTSQSWIRFRRVTRCTVFSSYKVSVNGQVVSNSVDSGLN
                     NDREFAKTTTEAKSFYLKSPQLNVNFTYQPPNSTAQGWLDWVELNISRRLVFRDGQFL
                     FTDPESAGKNKIAQFRIEGTNADVEVWDVTNPVNVHRMLATFTGGLTHFTTPVDSLRK
                     FVALDKTKFLKAEFVEKVANQNLHGISGVEMLIVAPDVFLSEAERLADHHRSFDGMQT
                     VVVPLNEIYNEFSSGAPDASALRDFCRYIYKDAGASVKLKYLLLFGDGSYDNKNRIAN
                     NTNFVPTFQTRESLITTDSYSCDDYYGLLDDTEGQDAAGLLDIGIGRFPVDDEAQAKS
                     AVDKVIYYSSKTPDVFGDWRNQLCLIADDKDNNMHIRQAEDNLFPIVKNNYPVYNINK
                     IYLDAYKMVSTPTGELYPDVNQAINAQMEKGALIINYTGHGGELGLAHEGVLTLQDIA
                     SWTNYENMPLFVTATCEFSRFDDPGRVSAGEQVFLHTSGGGIALITTTRLANSGYNIQ
                     LNMDLFDTIFSSVDKVYPRLGDVLAYAKNENNTPGAIRNFILIGDPALRLAYPKYQVL
                     TTGFNKQPVSAEADTARAMSKVELEGIVADAQGNLLPSFNGEVHVKVYDKAHWCMTLG
                     NSPESQPRYFEVQDNVLYQGKATVTNGKFSLTFVMPKDINYSFGNGKISYYAQSESTD
                     ANGYFDKVIIGGSEDGQMADTEGPVIKLYMNSMEFADGVVVGENPKLIAMLSDESGIN
                     TVGNGIGHDIVAVMDENADESTVLNEYYQSDIDSYQSGKVVYQYFKLDEGLHTLKLKA
                     WDVFNNSSEATITFTVKKDMAVSISDIKAYPNPFTDEVNVEFEHNHFDSSLDVILDVI
                     SVDGHLVRSLPAVTLVSSGNKAGPVKWNGCNESGRKVNGGMYLLRVRATDQNGSTGVE
                     TVKVIKASNPKM"
     gene            complement(5620..9438)
                     /gene="porU"
                     /locus_tag="PKI35_08960"
     CDS             complement(5620..9438)
                     /gene="porU"
                     /locus_tag="PKI35_08960"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNX44550.1"
                     /translation="MLKRIIELIGVLSLCLAPVFIYGQKTYRVTVNWHNPYTVQYEDG
                     SDGRYFDFDHMGVDPAWSLPVLIEHFSLPAGAGNIGLIVSDIKYIPLAAEEYGIVEAD
                     GIHLTDSALYNFRVSSARREKFAVLSLLPFRVNSSTGNVEKISGFSVSISPAPYRDQE
                     VKDLKHTYAGHSVLASGVWFKLAFTATGVCKLSYDDLVSIGMDPGSVDPRNIRIYGNG
                     GGMLPESNLVARYDDLVENAITVRGESDGSFDAGDCVLFYVAPQTKWNYNAQHGAWEH
                     VTNIYSDTAFYFLTAGAAAGKRIGEEYSTQLDETVRVNTFDDYLLHETDHVNLIGSGR
                     LWFGEIFDLTTQYSFDFNFPGIAEGSELTFNVQAAARSTSSSSFSFSAAGNSWPASAS
                     PVSTYYNSAYASQVNSFKNVPATTDQVTFAIAYNKPVSSAKAWLDFVEVNATRVLDFP
                     GGQLAFRKAQASGTGNVAQYQLGNVSKAVSVWDVTNPVNVRNVHALLTGTVMEFRLAA
                     DTIREFVAFDGNAFIHPVSHKQIPNQDLHAMQSPGLVIISPPVFMEQAVRLARHHESS
                     DDLSVSIVTPETIYNEFSSGSPDVSAIRDFMKMFYDRNQSSGTVPYLLLFGDGSYDFK
                     DFHKENTNYVLTFQSPESYDPVHSYTYDDYFGFLDDAEGCGAFDMVDAGIGRLPVKSL
                     AEATSAVDKIIHYSMQGGTVQGDWRNVITFVADDEEDNEHMLQADQLASYIGHNYANY
                     NIDKIYLDSYQQSATPSGNRYPDVNVAITQRIEKGTLIINYTGHGGETGWAHEEVLQI
                     SDINSWHNYDKMPVFVTATCEFSRYDDPERTSAGEQVFLNPLGGGIALFTTSRPTFGT
                     PNLAINKSIYKYALQSQPAATLRMGDIIREAKREGGSDENGRKFVLLGDPALRIANPE
                     LKVVTTQINGKAISEVPDTISALSAVTVSGIITDAYNVKATDFNGVLTSSVFDKATEV
                     TTLANDGGTPFTFSLQKNLLYKGHVEVHDGEFSFTFIVPRDISYRYGYGKISYYARSD
                     DKDAAGCYANLVVGGDGEDAQTDDEGPAIRLYMNDRYFVRGGTTDENPLLLAYISDES
                     GINTVGTGIGHDLIAILDDDYDNPYVLNDYYESEVNTYKSGMAQFPFVGLSEGLHTLT
                     VRAWDVYNNSSEGQTEFVVASSDNFIMENAVNFPNPFSNYTDITFSHNQQGKMLSSTV
                     TIFTLTGVPVVTLHQQGQDFGSRAVPVRWDGRTSSGNLAACGVYVYQIVSVTSDGLSA
                     KTSGKLIYTR"
     gene            9656..10645
                     /locus_tag="PKI35_08965"
     CDS             9656..10645
                     /locus_tag="PKI35_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNX44551.1"
                     /translation="MRTKLAFLTTLVILPSVFLWGQDHVFSQFYASPLYLNPALAGSK
                     ICPRLTLNYRNQWPGLGKGFVSYSASWDQQIDKISGGLGVIADADVSGGGALNTYSGS
                     AMYSYRLQASPKIVLNLALQAGYLQYSLNWDKLLFEDQIVPGTGEIIASSEQRPPDLS
                     IGTIDFATGILAGYNQRLYIGAAMHHITMPDMAFYTDDLNKLDVRYTIHAGAIFDIKE
                     GINGGDVEDLSFSPNIVYIQQGDFRQINAGLYVNLYPFVAGAWYRHTIDNPDAAIVLV
                     GFQHEKFKIGYSFDFTVSRLSIKSGGAHEVSFSWQFPCPVKEFKYKAIKCPRF"
     gene            10675..12165
                     /locus_tag="PKI35_08970"
     CDS             10675..12165
                     /locus_tag="PKI35_08970"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNX44552.1"
                     /translation="MAKHSKVVANLMFAATIALLASCSKPQSHTTGWDYNNPKNGGFE
                     VVPYEEPTTGPGLVFIEGGTFTMGRVSDDPMYEWNNIARRVTITSFYMDETEVSNLDY
                     VEYLYWLSRVFGTDYPEVCRKALPDTLVWREKLAFNEPLIETYLRHPAYRDYPVVGVS
                     WVQANDYCLWRTDRVNEMLLVREGILSFDPDQKNENNFNTEAYLAGQYEGLVNHAIPD
                     LNPNGTGERRVRMEDGIMLPKYRLPTEAEWEFAAYGLIGNTHFERIVERRVYPWNGTV
                     MRTDDKKYYGSFVTNFKRGRGDYMGVAGNLNDGAEIPAEVGSYWPNDYGLYNMAGNVS
                     EWVLDVYRPLSFEDVNDYNPFRGNVFKTKVTDPEGYLVPKDSLGRIKYREVTEEEAMG
                     RYNYRTANNINYLDGDYQSQVGVDWAQKSDDANITNKMYEYGVSSLITDKSRVYKGGS
                     WRDGAFYQSPSARRFLEEDMSTNYIGFRCAMGRVGSASMQSGKRSK"
     gene            12270..13562
                     /gene="murF"
                     /locus_tag="PKI35_08975"
     CDS             12270..13562
                     /gene="murF"
                     /locus_tag="PKI35_08975"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNX44553.1"
                     /translation="MRISLTDLYRIYSEHPVIITDSRKVIPGCLFFALKGENFDGNNF
                     AEQSLQQGAAFAITDNQAKTGEKILVVEDVLTTLQQLASVHRDTLAIPVIGITGTNGK
                     TTTKELANAVLSSHFTTHATSGNFNNHLGVPLTILSAPPATEVLIVEMGANHPGEITF
                     LCSLAKPTHGLITNIGKAHLEGFGGFEGVIKAKNELYQYLFSHQKHAFVNADNELLME
                     LSANLDRTLYGTTPGCSARGSVISSDPFLSVNIQGNSGNADIRTRLVGRYNFENVMAA
                     CCVGQSFGVPAEKMVKAVEAYEPGNSRSQAIDTLRNHIIVDSYNANPSSMKAALENFS
                     RLSAGRKMVILGDMFELGTESDAEHQAIVGLVEELGFDQVIFAGKEFMKAATGSSFTC
                     FPDSSDAFRWLKSNPPAGFTILIKGSRGSRMENVLEAL"
     gene            13587..14663
                     /locus_tag="PKI35_08980"
     CDS             13587..14663
                     /locus_tag="PKI35_08980"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNX44554.1"
                     /translation="MAKNRSAKEPFHVIGVMSGTSLDGVDLAYCSFTSGYQRLEFRIH
                     ACETVPYADFWKERLINLPSRDALTYAQTHVEYGHYLGRLVRSFIERNKLHAGFVASH
                     GHTIFHQPGKGFTSQIGDGAALAAECGLPVICDFRSTDVAFGGQGAPLVPIGDELLFG
                     DFDYCLNLGGFGNISFKKDGQRIAFDICPVNIILNRLAGMKGQAYDQNGNLAAGGKII
                     PGLLHELNNLPYYRQQPPKSLGREWLEKELLPLFSGAGVQTTDMLRTATEHIAMQISG
                     CLPSAGGRMIITGGGAFNKFLISRISDLCPVEIIIPDEKIINYKEALIFALLGTLRMQ
                     KKPNCLPSVTGALKAAIGGAVYLP"
     gene            14870..16225
                     /locus_tag="PKI35_08985"
     CDS             14870..16225
                     /locus_tag="PKI35_08985"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNX44555.1"
                     /translation="MKKSTLLRIAGLSALLVLAPVMTFAATTATMPMVFGIRIEFILF
                     ALTLVGVAVFHHKTMYVALIGLAAILITKYIFMPGEFSLIDHIIGTPGKEGEWRILLN
                     LLGLLLGFAILAKYFEESGVPDWLPRVLPNDWKGGLVLLFAIMFISSFLDNIAAAMIG
                     GAIAMVVFKGKVHIGYLAAIVAASNAGGAGSVVGDTTTTLMWIDGVNPLDVTHAFIAS
                     IVAFCIFGTIGSIQQDRYQHIQKDESKGYHIDWGKLGIVLLILVCAILTNWLLDFPAA
                     GVWIAILIGTFIRRAPWNEIPKSIQGTIFLMALVTCASLMPVDQLPAASWQTAFSLGF
                     VSAVFDNIPLTKLCLDQGGYDWGILAYTVGFGGSMIWFGSSAGVALSNMYPEAKSVIK
                     YVKHGWHVSLAYIISFFVMLGIVGWHPHAPHRKEHQLQQQELANPPAAATVNDTTAQV
                     N"
     gene            16400..17128
                     /locus_tag="PKI35_08990"
     CDS             16400..17128
                     /locus_tag="PKI35_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX44556.1"
                     /translation="MIPGVILQIVQGTQTLADTVVQAVNTLPQVHQPTEIKLSIWDLA
                     IKGGPVMIPIALLSVIAIYIFVERYFVIGKASKEDANFMNNIRDFMLNGRLDSALSLC
                     RNNQTPIARMIEKGLLRLGRPLGDINAAIENVGSLEVAKLEKNLSLLATCAGAAPMLG
                     FLGTVTGMVSAFYDMSMAGNNIDIQLLSSGIYEAMVTTVAGLIVGILAYFCYNLLAAR
                     VQKIVFLLEARATEFMDLLHEPAK"
BASE COUNT         4481 a         4282 c         4034 g         4446 t
ORIGIN      
        1 cggtggtcgc cgtatttttc aaaaaatcag ccaccggaat gccaaggatg tcggcaatga
       61 tacggcgcat ggtgtcaatg taaggcgcaa cctttggcgc ctggagtgca attgtggaat
      121 caatatttac aacactgtaa ccacaggaat tcagcatatc cgaaactctc cggagcaaaa
      181 ttttactgtc aatatctttg agcgacggat cggtatcggg aaaatgtttc ccgatatctc
      241 ccaatgcggc cgcccccaac agggcgtcgc aaacggcatg caacagcaca tcggcatccg
      301 aatgtccatc agcgcctgtt tcagacggta tcctgactcc tccgagccaa aattcacgtc
      361 cggctaccag ccggtgaacg tcgtagccaa aacctatccg ctggttcata gtcctgtgat
      421 tttctgcaaa tataaggttt ccgccatttt ctgacagggc aaaaaaaatc cgggtagaat
      481 gcccggatca gatttctttg agaaggttat ttctttcccc cgccactgac agcttcaaaa
      541 ttcagcagca atgaaaaacg gagggtattt tccaggggat tatccgttgc caggggtacc
      601 agataggaaa catcgagccc gaaaacattg tacctgaggc ctgcgccaag ggtcacatag
      661 gtacggtcgc ctttggtagg atcttcataa aagaacccgc cgcgaacggc gaattgctgg
      721 ttataccaat attcaacccc ggttgaaata tttatttcac gcaactcttc actgaagccg
      781 tcgggggcat catagaacga atgcagcatc cctacggctg ttgaaacatc cggatccata
      841 cccctgacaa ttaccgttga attggtatcg ctgtactccg gggtggtagg taccagcaac
      901 ttgttaaaat cgagcatgaa cgacatgcgg ttgtactcgt cgaggtcaag ggtcagcgat
      961 gacccgatgc gcaggttggt aggaataaaa tccttccgta tattatcgtt gctgtatgaa
     1021 atcttggccc cgatgtttga aatattcacg ccccaggcaa acagtccttc cgtgctgcta
     1081 aactgcaatg gaagttgctg gtataccgaa acatcggcag ctaccgaatt tccgggtttt
     1141 gttgactggc tcccgacatc ctgcctgagg gtgaggtttg aatggataaa gcgggcagcg
     1201 acagatcctg aaagggtttt tgtgaatttt ctggagtatg cgaagtcaag ggcgaattcg
     1261 ttgggtttgt aatcgccgag aggttctccg aactgactgg taaactggat attccctaat
     1321 gagaaatagc gaagcgaggc agctatcgtc tggtttttat caagcctcga atagcctgaa
     1381 agatatgcca ggctgatatc gggaaccagg ttgtgaagcc atggactata attaacagca
     1441 acccccattt gtttatcaat gaatgcatat ttggcgggat tccagtgcat ggaattttcg
     1501 tcgggggttg aggcaacacc ggcatcaccg agcgatccgg aacgggcatc gggagatacc
     1561 atcagaaacg gaacggctga ttgaacagta ttaagcccct gcgccatcga catagttgcg
     1621 gagatcatcg ccgccagcaa tgtaaagaag aacacacact ttttattcaa catgtcttaa
     1681 agattaagaa aagaatgcaa atttaaactt tttgatttaa cggatgtaaa acgacactcc
     1741 cggtaagtta ttgcatccgc agacaaattt ggcccctaca ttttcggatt ggaggccttg
     1801 atcactttca ccgtttcaac gccggtactt ccgttttgat cagttgccct gacgcgcaac
     1861 aggtacatac cgccgtttac tttacgtccc gattcgttgc aaccattcca cttcacaggg
     1921 ccagccttgt ttcccgatga aacaagcgta accgccggta aggaacggac cagatgcccg
     1981 tcaaccgaga tgacatccag gatcacatca agggatgaat cgaaatggtt atgttcaaat
     2041 tcaacattca cctcgtcggt gaaagggttc ggataagcct tgatatcgga tatactcaca
     2101 gccatatctt tcttaacggt gaacgtgatg gttgcttccg acgaattgtt gaatacatcc
     2161 caggctttta gcttcagggt atgaagtcct tcatcgagtt tgaagtactg gtacacaact
     2221 ttcccgctct gatagctgtc gatatcggac tggtaatatt cgttaagcac agtcgattca
     2281 tcagcatttt catccatcac agccacaata tcgtggccta tgccattgcc caccgtgtta
     2341 atcccgcttt catcgctcaa catagcgatg agtttcgggt tttcgccaac aacgacaccg
     2401 tcggcaaatt ccatgctgtt catgtacaac tttattaccg ggccttcggt atccgccatc
     2461 tggccgtctt cggaaccgcc gatgatgacc ttatcaaaat acccgttggc atcagtactt
     2521 tcactttggg cgtaatagga aatttttcca ttcccgaagc tgtaattgat atccttcggc
     2581 attacaaacg tcagtgaaaa tttgccattc gtaacagtag ccttgccctg gtacagaaca
     2641 ttatcctgta cttcgaaata acgcggctgg ctttccggac tatttcccag cgtcatgcac
     2701 caatgcgctt tatcatatac tttcacatgg acctcaccgt tgaaggaggg taaaagattg
     2761 ccctgggcat cggcaactat gccttccagc tcaactttcg acattgccct ggcagtatcg
     2821 gcttcggccg aaaccggttg tttgttaaat cctgtggtga gcacctggta ttttggataa
     2881 gccagcctga gcgccgggtc tccaatcagg ataaagttgc ggatggcccc gggggtatta
     2941 ttttcatttt ttgcataggc aagaacatca cccagacggg ggtatacttt atcgaccgat
     3001 gaaaaaatgg tatcgaacaa gtccatgttc aactggatat tataaccgga gttggccaga
     3061 cgcgttgttg ttatcagggc gatccctccc cctgaagtat gcaggaacac ctgttcccct
     3121 gccgaaaccc ttcccgggtc atcgaaacgg ctgaattcgc aggtcgccgt gacgaataac
     3181 ggcatgtttt cataattggt ccagcttgca atatcctgta aggtaagaac cccttcatgc
     3241 gccagtccca gttcgccccc gtgcccggta taatttatga taagcgcccc tttttccatc
     3301 tgtgcattga tggcctggtt tacatcgggg tacaactcgc ccgtaggtgt cgataccatt
     3361 ttataagcgt cgaggtatat cttgtttata ttataaaccg ggtaattgtt tttaacgatc
     3421 ggaaacagat tgtcttcagc ctgccggata tgcatattgt tgtccttgtc atcagcgatc
     3481 aggcacagtt gattgcgcca gtcgccgaaa acatcaggcg ttttcgaaga ataatagatt
     3541 accttatcga cggccgattt tgcctgggct tcgtcatcaa ccggaaaacg cccgatcccg
     3601 atatcaagaa ggcctgcggc atcctgtccc tcggtatcat ccagcaaccc gtaataatca
     3661 tcgcaggagt aagagtcagt agtaatcagc gattcgcgcg tctggaaggt cggtacgaag
     3721 ttggtattat tggcaatcct gtttttgttg tcataggaac catcgccgaa taacaggagg
     3781 tatttcagtt ttaccgacgc accggcatcc ttataaatat accggcaaaa atcacgtaat
     3841 gcagacgcat ccggcgctcc cgaggaaaac tcattgtaaa tttcattcaa cggaaccacc
     3901 actgtctgca ttccgtcgaa actgcggtga tgatctgcca gccgttcggc ttcggacagg
     3961 aaaacatccg gcgccacaat cagcatttca acgcccgaga tgccgtgaag attctggttg
     4021 gcaactttct caacaaactc cgctttcagg aattttgtct tatccagggc tacaaatttc
     4081 ctgagagaat ccaccggcgt tgtaaaatgg gtaagcccgc cggtaaacgt ggcaagcata
     4141 cggtgaacat tcacggggtt tgtaacatcc catacctcca catcggcatt tgtgccttct
     4201 atcctgaact gggcaatttt attctttcct gcgctttccg gatccgtgaa caggaattgc
     4261 ccgtccctga atacaagtct gcgcgatatg ttcagttcca cccagtcgag ccatccctga
     4321 gccgtactgt taggaggttg ataggtaaaa ttgacattga gctgcggcga ttttaaataa
     4381 aagctctttg cctcggtcgt tgttttggca aactcacggt cgttgttcaa tccgctgtca
     4441 acactgtttg aaaccacctg accattgacc gacactttat aactggaaaa aacagtacac
     4501 ctggttaccc gcctgaaacg tatccacgac tggctggtgg aatcaaaatc gggaaatgta
     4561 taggcaggaa ggttgacctg gttagaactc atatccatcc gttcccctac ccaataccta
     4621 cctgatttta tcagattata aagatcgttt tcataaaaca tgaaatccgt atacctggtg
     4681 acgatttccg aaggcggcag ggtagtctgg gctatgtcat cgatccgttt tccatttccg
     4741 ccgaacgtca gaaaatagca ggagtaatcg ctatagtcgt ttttctcatg gtcgaagcgc
     4801 ttggtaagct gatcatagtt aaccttatcg ggcccggttg caaaaaagca gatataatcg
     4861 cccgatttaa aagtgcccgg ttgagaagtc actacctgaa tagcattttc gagaagatcg
     4921 gatgcacgcg gcttgttatt tctttcagga agcatacctc ctccattgcc gaaaagcctg
     4981 atattatccg gcgtaacttc agccatattc acgcccatgg cctttatttc atcataggta
     5041 agagagtata cgcctgtttt atccagccgg attttatacc agttgcccgt ggcaagcacc
     5101 gaatttatcg aaccggctga acttttcact aacgcggttt ccgttacggg ttccgtttcg
     5161 attatcaggt cgaatgagac cagtttctca ggagcggagc cttgaagccg caaagtgctg
     5221 actgacactg tcatcacagg cttttcgcgt tccgtccaga aattatattc cagactaatg
     5281 gaatcggaaa catccggctg gaacgtcaac tgtttcagtt cggacggctc gacccgttca
     5341 tataccgtgt tttcgatgga cacccgtacc gggacttcat cattattaaa cctgaacact
     5401 ttaaagaaga agggagcata ccctttgccg gcaaggtttg cagcatcgtc gaaatataaa
     5461 acattcaagg gttcggaaga ttcgttcccg atgttggtga ccgcttgcca ttgcagcctg
     5521 acagcatgtc gcgtgacatt tgcccctgct aatagcggta gaagaaacat gaatactaaa
     5581 atacaggcag atctgttcat aatggttaac tgttaaaatt tatctggtgt aaatcaattt
     5641 tcctgatgtt tttgccgaaa gaccgtccga cgttactgat acaatttgat acacataaac
     5701 cccgcatgcg gccaggttgc ccgaggatgt ccgcccatcc caccggacag gaacagcccg
     5761 tgagccgaaa tcctggccct gctgatgcaa ggtaactacc ggcaccccgg tcaaggtaaa
     5821 aattgtaacg gttgacgaga gcattttccc ctgctggtta tgactaaagg taatatcggt
     5881 gtaattgctg aaagggttcg ggaaattaac ggcattttcc ataataaaat tgtccgacga
     5941 agccacaaca aattcggtct gaccttccga cgaattatta taaacgtccc aggcccttac
     6001 ggtcaacgta tgcagccctt cggacaaccc gacgaacgga aactgcgcca tccctgattt
     6061 ataggtatta acttccgact cgtagtaatc attcagcaca tacggattgt cgtaatcatc
     6121 atcaagaatg gcgatcagat cgtgtccgat gcctgtaccc accgtgttga taccgctttc
     6181 gtcgctgata taagccagca acaaagggtt ttcatcggta gtgccgcctc tgacaaaata
     6241 ccggtcattc atatacaacc tgatagcggg gccttcatca tccgtttgcg catcctcgcc
     6301 atcgccgccg accaccaggt tagcataaca cccggctgca tctttgtcgt cactccgggc
     6361 gtagtaactt atcttcccgt atccgtaacg ataagaaatg tcgcgcggaa cgataaacgt
     6421 aaacgaaaac tccccgtcgt gcacttctac atgtccttta taaagaaggt tcttctgcag
     6481 gctgaaggta aatggcgttc ctccgtcgtt tgcaagcgtc gtcacctctg tagccttgtc
     6541 aaaaaccgaa gaagtgagta ccccgttgaa atctgttgct ttcacattat aagcatcggt
     6601 tataattccc gaaaccgtaa ccgcggaaag agcgctgatt gtatccggga cctcactgat
     6661 tgccttccca ttgatctgtg tggttaccac cttcaattcg ggattggcga tacgaagagc
     6721 cgggtcgcca agaagcacga actttctgcc gttttcatcg gaaccgcctt cgcgtttagc
     6781 ctccctgata atgtctccca tcctcaacgt agctgccggc tgggactgca gggcatactt
     6841 ataaatactt ttattaattg cgaggttggg cgttccgaaa gttggccgcg atgtggtgaa
     6901 tagggcgatt ccaccgccca gcgggttcag gaaaacctgt tctcccgccg aggtcctttc
     6961 cgggtcatcg taacggctga attcacatgt ggcggttaca aaaacaggca ttttgtcata
     7021 attatgccat gagttgatat cgcttatttg cagtacttct tcgtgggccc agcctgtttc
     7081 gcctccatgc ccggtgtagt tgataatcag ggtccctttt tcaatgcgct gggttatggc
     7141 gacgttgacg tcgggataac ggttgccgct tggcgtcgcc gactgctggt aggaatcaag
     7201 gtaaatttta tcgatgttat aatttgcgta gttatggcca atgtaacttg ccagttgatc
     7261 ggcttgtaac atatgttcat tgtcttcttc atcgtcggca acaaaggtga tcacgtttcg
     7321 ccagtcaccc tgcaccgtgc ctccctgcat ggaataatgt atgattttat caacggccga
     7381 cgttgcttca gccagcgatt tgacaggaag ccttcctatc ccggcatcaa ccatatcgaa
     7441 tgcgccgcag ccttccgcat catcaagaaa tccgaaataa tcgtcgtagg tgtaagagtg
     7501 caccgggtcg taggattcag gcgactgaaa cgtcagtaca taattggtat tctctttatg
     7561 aaaatccttg aaatcgtatg atccgtcgcc aaatagcaac agataaggga cagtccctga
     7621 cgattggtta cgatcgtaaa acattttcat gaaatcccgg atggcgctga catcgggcga
     7681 tcctgaagaa aactcattgt agatggtttc cggcgtgacg atagaaactg agagatcatc
     7741 agatgattca tgatgccggg caagcctgac agcctgttcc atgaagacag gcggagatat
     7801 gatgaccagg ccgggagact gcatggcatg gagatcctga ttgggaatct gcttatgact
     7861 gaccggatgg ataaaggcgt tgccgtcaaa ggcaacaaac tcacggattg tatcggccgc
     7921 aagtcgaaat tccatgactg taccggtcag aagtgcatga acgttgcgaa cattaaccgg
     7981 attagtaaca tcccaaacgg aaaccgcttt cgatacgttt ccgagctgat actgcgcaac
     8041 attccccgta ccggaagcct gtgctttcct gaaggccagt tgcccccccg gaaagtcaag
     8101 cacacgcgtg gcattaacct caacaaaatc aagccaggct ttggctgaac ttaccggttt
     8161 attgtaggca atcgcaaacg ttacctggtc ggtagtagcc ggtacatttt taaagctgtt
     8221 tacctgtgag gcataagcac tgttataata ggtagaaaca ggcgatgctg atgccggcca
     8281 tgaattaccg gcagccgaaa aggaaaagga gcttgaagaa gtgctccggg ctgctgcctg
     8341 aacattgaaa gtcaattcgg aaccttcagc gataccggga aaattaaaat cgaatgaata
     8401 ttgtgttgtc aggtcaaaaa tttcgccaaa ccacagcctt cccgatccga tcaggttaac
     8461 atggtcggtt tcgtggagca ggtaatcgtc gaatgtattc acccggacgg tttcatccag
     8521 ttgggtgctg tattcttccc cgatacgttt accggcggcg gctccggcag taagaaaata
     8581 aaaggccgtg tcagaataaa tgttggtgac atgctcccag gcgccgtgct gggcattata
     8641 attccacttt gtctgcggcg cgacatagaa aaggacacag tcgcccgcat cgaaactccc
     8701 gtccgattcg ccccttaccg tgatggcgtt ctcaaccaga tcgtcatatc ttgccacgag
     8761 gttgctttca ggaagcatgc ctccgccgtt tccgtagata cggatattcc ggggatccac
     8821 agagcccgga tccatcccta tagaaaccag gtcatcataa gataatttgc atacgcccgt
     8881 agcggtaaag gccagcttaa accatacgcc cgaggcaaga accgaatgac cggcataggt
     8941 atgtttcaaa tcctttacct cctgatcgcg ataaggcgcc ggagaaatgc taaccgaaaa
     9001 accggaaatt ttttctacat ttcctgtaga ggaatttacc ctgaagggca gcaaacttaa
     9061 aacggcgaat ttttcccgtc ttgccgaaga aacccggaaa ttataaaggg ctgaatcggt
     9121 cagatgaatg ccgtccgctt caacaatacc atactcttca gccgccagcg ggatatattt
     9181 gatatccgaa acgatcaacc cgatgttccc ggcaccggca ggcaggctga agtgttcaat
     9241 gaggacaggc aggctccatg caggatcaac tcccatatga tcgaaatcaa agtatctgcc
     9301 gtcagaacca tcttcatact gtactgtgta tggattatgc cagttcactg tcaccctgta
     9361 cgtcttctgc ccgtaaataa atacgggggc caggcacagg ctcagcacac caattaattc
     9421 aataatcctt ttcaacatgc agttactttt tacggattcg atgataaaat aataaaaatg
     9481 aataccctgc gttctttgaa tacgttagta aaacaaatgt cgtgcgtgta agttcaacgt
     9541 agcggggaac ataacactaa acggagaatg ggccgtttta ttaccaggaa ggtatttttt
     9601 cacttgcatt tatacctttc aaagaaattt cttcgtaata ttgacgccct tgaatatgcg
     9661 aacaaagtta gcatttctga caacgctggt tattttgccc tcagttttcc tctggggaca
     9721 ggatcatgtt ttctcgcagt tttacgccag tcccctctat ctgaacccgg ctcttgccgg
     9781 ttccaaaata tgcccccgcc taacgctgaa ctaccggaat caatggcccg gcctggggaa
     9841 agggttcgtt agttacagcg cgtcctggga ccagcagatt gataagatat caggaggcct
     9901 gggcgttatt gccgatgctg acgtgagtgg cggcggagca ttaaatactt acagcggaag
     9961 cgccatgtac tcttacaggt tgcaagcctc gcccaaaatt gtcctaaatc ttgccttaca
    10021 ggccggttac cttcaataca gcctgaattg ggataaactg ctctttgagg accagatagt
    10081 tcccggcacc ggcgaaatca tcgcatcctc ggaacaaagg ccgcctgacc tgagtatcgg
    10141 gaccatcgat tttgcaacag gcatccttgc cggatacaac caacggcttt atataggcgc
    10201 tgccatgcat cacatcacca tgcccgatat ggctttttac accgatgacc tgaacaagct
    10261 ggacgtacga tatacaatcc atgcaggtgc aattttcgac ataaaggaag gaataaacgg
    10321 cggcgatgtt gaagatttat ccttctcacc aaacattgtt tacatacaac aaggcgactt
    10381 caggcaaata aatgcaggat tgtatgtaaa tttgtacccg tttgttgccg gagcctggta
    10441 ccggcatacg atcgataatc ctgatgcagc catcgtactc gttggctttc agcatgaaaa
    10501 attcaaaatc ggatacagtt tcgatttcac tgtatcaagg ttaagcataa aatccggggg
    10561 ggcacatgaa gtatcttttt catggcaatt cccttgcccg gtaaaagaat tcaagtacaa
    10621 ggcaataaaa tgcccgcgtt tttagttata acgatcaata aaccataaac tgcaatggca
    10681 aaacacagta aagtcgtagc aaatttaatg tttgcagcaa cgatagctct gctggcttcc
    10741 tgctcaaaac cacaatcgca tactaccggt tgggattaca ataatccgaa aaacggcgga
    10801 tttgaggttg taccttacga agaaccgaca acagggcccg gtcttgtatt tattgaaggc
    10861 ggtactttca ccatgggccg cgtgagcgat gatcctatgt atgaatggaa caatattgcg
    10921 cgcagggtta ccatcaccag tttttatatg gatgaaactg aagtgtcgaa cctcgattac
    10981 gttgaatacc tctattggct gagccgtgtc ttcggcaccg attatcccga agtatgccgc
    11041 aaagcattgc ccgacacgct cgtatggcgt gaaaaactgg ccttcaatga gcctctgatc
    11101 gaaacctacc tgcgccatcc tgcctaccgt gattacccgg ttgtaggcgt aagctgggtt
    11161 caggccaatg actactgcct ctggcgtacc gacagggtta acgaaatgct gctcgtccgc
    11221 gaaggtatcc tttcatttga ccccgatcag aaaaacgaga ataacttcaa cactgaagcg
    11281 tacctcgctg gacaatatga gggcttagtg aaccacgcaa tacctgatct taatcccaat
    11341 ggcaccggcg aacgccgcgt aagaatggaa gacgggatca tgttgcccaa gtaccgcctc
    11401 ccgaccgaag ccgaatggga atttgccgcc tacggcctca ttggcaacac acatttcgaa
    11461 cgtattgttg agcgccgtgt gtatccctgg aacgggaccg tcatgcgtac cgatgataaa
    11521 aaatattacg gcagttttgt caccaacttc aaacgcggac gaggcgacta catgggcgtt
    11581 gccggaaacc tgaacgacgg cgccgaaatt cctgccgaag tgggttccta ctggcctaac
    11641 gattacgggc tgtacaacat ggcaggcaat gtttcggaat gggtgctcga cgtttatcgt
    11701 ccgctgagtt tcgaagatgt caatgactat aatcctttcc gtggaaacgt ctttaaaacc
    11761 aaagtcaccg atcctgaagg atacctcgtt ccgaaagaca gccttggccg cattaaatat
    11821 cgtgaagtca ccgaagaaga agccatgggc cgctataact accgtactgc caacaatatc
    11881 aactatcttg acggtgatta ccagtcacag gtaggcgttg actgggctca gaaaagcgac
    11941 gatgcgaaca tcaccaacaa gatgtatgaa tatggcgttt cgtcgctcat taccgataaa
    12001 agccgcgttt ataaaggcgg ttcctggcgc gacggggcat tttaccagag cccttcggcc
    12061 cgccggttcc tcgaagagga tatgtcgacc aattatatcg gcttccgctg cgccatgggc
    12121 cgggtaggaa gcgcttcgat gcaatcgggc aaacgaagta aataattaaa ccattctttt
    12181 tataaaaccc gttagcatcg cataacggtt ttttttttgc cgtccgttcc ttatttgtac
    12241 cttcgttact cgtttataat gccgtattca tgcgcatctc actcacggat ctttaccgga
    12301 tttattcaga acatcccgtc atcattaccg attcgagaaa agtcataccc ggatgtcttt
    12361 tctttgccct caaaggggaa aactttgatg gaaacaactt tgcagaacaa tccctgcagc
    12421 agggagcagc cttcgccatt accgataatc aggctaaaac cggagaaaaa atactggtag
    12481 ttgaagatgt cctgacaacc ttacagcaac ttgcatctgt tcatcgcgac accctggcaa
    12541 tccctgtaat cgggattacc ggaaccaacg gtaaaaccac gacaaaagag ctcgccaacg
    12601 cggttttgtc atcccatttt accacgcacg ccacttccgg caatttcaat aaccatctcg
    12661 gcgttccttt aaccatatta tctgcgcctc cggcaacaga ggtgcttata gtggagatgg
    12721 gcgccaatca tcccggggag atcacctttt tatgcagcct ggctaaaccc acccatgggc
    12781 tgatcaccaa tatcggcaaa gcgcacctcg aaggattcgg gggatttgag ggagtcatca
    12841 aagctaaaaa cgaactctac caataccttt tctctcatca gaagcatgct tttgtaaacg
    12901 cagacaatga attgctgatg gaactatccg caaacctgga caggacgctt tacggaacca
    12961 ctccgggttg ttcagcaagg ggcagtgtca tatcttccga tcctttcctt tcggtaaata
    13021 tccagggcaa ttcaggcaat gcagatatcc gtacccgcct ggtcggccgg tataatttcg
    13081 aaaatgtaat ggcagcttgc tgtgtcggac aatcgtttgg agtgcctgca gaaaaaatgg
    13141 taaaggctgt tgaagcctat gagcccggca acagccgttc acaagccatt gacaccttaa
    13201 gaaatcacat cattgttgat tcatacaacg ccaatcccag cagcatgaaa gccgcgcttg
    13261 aaaacttcag caggctgagt gccggaagga aaatggtaat tctcggcgac atgtttgaat
    13321 tagggactga aagcgatgct gaacaccagg ctatcgttgg tctggtcgaa gaacttggat
    13381 tcgaccaggt tatctttgcc ggcaaagaat tcatgaaagc agccaccggc tcttctttca
    13441 cctgctttcc cgattcatcc gatgctttca ggtggctgaa atcaaatccg cctgccggtt
    13501 tcactatcct gataaaagga tcccgcggaa gcaggatgga aaatgttctg gaggcgttgt
    13561 aaccggaaaa caaatgcgtt ttcagtatgg caaaaaacag atcagcaaaa gagccttttc
    13621 atgttatcgg ggtaatgtcg ggaacttcgc ttgatggcgt agaccttgcg tattgttcgt
    13681 tcacatccgg ataccagcgt cttgagttca ggatacatgc ctgcgagacg gtgccttatg
    13741 ccgatttctg gaaagagcgg ctgataaatc tgccttcccg tgatgcttta acctatgctc
    13801 aaacgcatgt ggaatacggg cattacctcg gccggttagt cagatcgttc atcgaaagga
    13861 acaagctgca tgccggattt gtcgccagtc acggacatac catatttcat cagccgggca
    13921 aggggttcac gtcacagatc ggcgatggcg cggcactggc tgcggagtgc ggtttaccgg
    13981 tgatttgcga ttttcgatcc accgatgtgg ccttcggcgg acagggggct ccattggtac
    14041 ccatcggcga tgaactgctt ttcggagatt ttgattactg ccttaacctg ggcggattcg
    14101 ggaatatatc ctttaaaaaa gacggtcaac ggattgcctt tgacatttgc ccggtaaaca
    14161 taatcctgaa caggctcgca ggaatgaaag ggcaggcgta cgaccagaac ggcaatcttg
    14221 cggctggtgg aaaaataatt cccggcctcc tgcatgaact gaacaatctt ccctattatc
    14281 gccagcaacc gccaaaatcc ctggggaggg aatggcttga aaaagagtta cttcctctat
    14341 tttcaggcgc cggagttcag accaccgata tgctccggac agcaactgaa catatcgcaa
    14401 tgcagataag cggttgtttg ccttctgccg gtggaaggat gataataacc ggcggaggcg
    14461 ctttcaacaa atttctgatt tcccgtatct ctgatctttg cccggttgaa atcattatac
    14521 cggatgaaaa gattatcaat tataaagaag ctctcatttt tgcgctcctg ggaacgctca
    14581 ggatgcagaa aaagccgaat tgccttccat cggttacggg cgcccttaag gcagcaatcg
    14641 gaggcgccgt ttacctgccc tgagcaagca cagagaacct tgaattttct ttagacagga
    14701 attgcaagta tggataaatc tgtacctttg acgcaaaatc aggtgtaagg aaggttaaaa
    14761 tcttgtcatt ctatttaatt gttttgcaag tatttaaatg acaaatttta aatttcaggg
    14821 cacaaactgt cgacgacaaa ttcctcatga aacaactaaa gtaaccttta tgaaaaaatc
    14881 taccttgttg cgtattgccg ggctgtcagc cttacttgtc ctggcgcctg tgatgacgtt
    14941 tgcagccacc accgccacca tgccgatggt atttggcatc aggattgaat tcatactctt
    15001 tgccctgacc cttgtcggcg ttgccgtttt ccatcataaa accatgtatg tggcgctcat
    15061 cgggcttgcc gccatcctga tcacgaaata catattcatg cctggtgaat tttcacttat
    15121 tgaccatatc ataggcactc ctgggaaaga gggagaatgg cgcattttat tgaacctgct
    15181 cggattactg ctcggatttg ccattctggc caaatacttc gaggaatcgg gcgttcccga
    15241 ctggctgcca agagtgcttc ccaatgactg gaaggggggg ctggtattgc tgttcgccat
    15301 aatgttcatc tcgtcgtttc tcgataatat cgccgctgcc atgattggcg gggccatcgc
    15361 catggttgtc ttcaaaggca aagtgcatat cggttacctt gccgccatcg tggcagccag
    15421 taatgcaggc ggcgccgggt cggttgtcgg cgacactacc acaaccctga tgtggattga
    15481 cggcgtcaac ccgctggatg taacacacgc ttttattgca tcaatcgtgg cattctgtat
    15541 tttcgggacc attggttcca ttcagcagga ccgctatcag cacatccaga aagacgagtc
    15601 gaaaggatac catattgact ggggaaaact cggcatcgtt ctgttgatcc tggtttgtgc
    15661 catcttaaca aactggctgc ttgatttccc ggccgccggc gtttggattg ccatcctgat
    15721 cggaactttt atccggagag ccccctggaa tgaaatcccc aaatccatcc agggaacgat
    15781 atttctgatg gccctggtta cctgtgcatc gttgatgccg gtagaccagt tacctgccgc
    15841 ttcctggcaa actgccttct cgctgggctt tgtttcggct gtatttgata atattcctct
    15901 cacaaagctt tgtctcgatc agggcgggta cgactggggt attctggcct atacggtagg
    15961 ctttggtggt tccatgatct ggttcgggtc ctcggccggc gttgccctgt cgaatatgta
    16021 ccctgaagcc aaatcggtaa taaaatacgt caaacacggc tggcacgtct cgcttgccta
    16081 tatcatcagt ttctttgtta tgctgggcat tgttggatgg catccccacg ccccccatcg
    16141 caaagaacac cagttacagc aacaggaatt ggcaaaccca ccggccgctg cgactgtaaa
    16201 tgatacaaca gcacaggtca actaaacggg cttttggttc gttggcgcaa tgcttgtcct
    16261 tttttaacgg aatcatgccg cgataaaata attttccttc tgacgagtta ttattaacag
    16321 cccgatgttt acaattaaca ccgggctgtt agtacctgtt actcattgtt atctaatttt
    16381 aaggattaaa atcagcaata tgatacccgg agtcatcctt cagatcgttc aaggcacaca
    16441 aacattggcc gataccgttg ttcaggcggt gaatacgctt cctcaggtgc atcaacccac
    16501 cgaaataaag ctttcaatct gggacctggc catcaaagga ggaccggtga tgatacccat
    16561 tgccctgctc tccgtcattg ccatttatat tttcgtcgaa cggtatttcg tcatcggtaa
    16621 ggcctcgaag gaggatgcca attttatgaa taatatccgc gatttcatgc tgaacgggcg
    16681 tctcgattca gccttgtcgc tatgccgcaa taaccagacg cctatcgccc gcatgattga
    16741 aaagggttta ctgcgacttg gacgtccact gggagatatc aatgccgcta tcgaaaacgt
    16801 tggaagtctt gaagttgcca aactcgaaaa gaacctttcc ttgctggcca cttgcgccgg
    16861 agcggctccc atgctgggat ttttaggaac cgttaccggt atggtaagcg cattttacga
    16921 tatgtcgatg gccggcaata acatcgatat ccaattgctt tcgagcggta tttatgaagc
    16981 catggttact accgttgccg gtctgattgt tggaattctg gcatatttct gttataactt
    17041 actggctgcc agggtccaaa aaatcgtatt cctgctcgaa gcccgcgcta ccgaattcat
    17101 ggacctgttg cacgaaccgg caaaataagc cttctgtccc gggtcgcacg gcagtaagtc
    17161 cgacaattat cgtttgcagt aaatcagagc atcaaaccca agatcaatgg caataaaatc
    17221 gcacaataaa gtaagcctgg aat
//
LOCUS       DAOPYV010000066        16990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_3572_length_16990_cov_13.250133, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000066 DAOPYV010000000
VERSION     DAOPYV010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16990
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3572_length_16990_cov_13.250133"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1072)
                     /locus_tag="PKI35_08995"
     CDS             complement(104..1072)
                     /locus_tag="PKI35_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNX44557.1"
                     /translation="MISFKSDTEALDAFTLKYNELRNEIGKVIIGQDEAIKNLIIAIF
                     SKGHGLLVGVPGLAKTLMVNTISKVLGLSYSRIQFTPDLMPSDIIGTEILDENRQFRF
                     IRGPVFANIILADEINRTPPKTQSALLEAMQEKAVTVAGMSHKLEEPFFVLATQNPIE
                     QEGTYPLPEAQLDRFMFNIWLDYPSFEQEIRIVKTTTANYLADLKVVLQRDEIIFFQD
                     LIRRIPVAENVFQYAVKLASRTRPGTPAAHAWANDYLTWGAGPRASQYLILGAKCHAA
                     VNGKYSPDIEDVQAIANSILRHRIVRNYKAEAEGIKEDHIVQELLK"
     gene            complement(1151..2497)
                     /locus_tag="PKI35_09000"
     CDS             complement(1151..2497)
                     /locus_tag="PKI35_09000"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX44558.1"
                     /translation="MKKFFFTALFLIPIFTTIPVKAQDKVIDEVVAVVGSHPVLYSDV
                     EVQYMQMKAQTTVPDPAAARCQIFEQLLFQKLLLYQAEVDSIVVDDEQVNATLDQKLR
                     YYIQQFGSQEKLEDFYDKSILEIKEEFREPIREQMLSEQVQRKITENIKITPTEVKKF
                     FEKIPADSIPVIPMKYEIAQIVKEPDVSSEELNASREKITALRDRILKGEKFSTLAVL
                     YSEDPGSASKGGELGFFGRGDMAPEFEAAAFSLKNKDDMSEVIRTKFGFHIIQLIERR
                     GELINARHILVIPKVSPLDLSRAKNELDSIAGLIAKDSISFEKAALKFSDDPGKVNNG
                     LLINQASNNSVFPADQLDPKIFFVIDKLDVGGVSAAVPYSTEDGTSAYRILKLVSRTQ
                     PHKATLKDDYDQIQEWALNEKKGKAVTKWIADKSKTAFIRIDDKYKNCPFQYNWNL"
     gene            complement(2513..3361)
                     /locus_tag="PKI35_09005"
     CDS             complement(2513..3361)
                     /locus_tag="PKI35_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44559.1"
                     /translation="MPKLFLFLPIVLLASSCSLFSGHGSKKEAVARVNDTYLYRDDLA
                     DVVPAGTDEKDSADLVKTYINNWIRQQLLLQQAENNLSSDKSDFTKELEQYRNSLIMY
                     EYESELVKQKLDTVISEKEIVDYYNKNQANFQLRENIVKACYIIAPLKTREIPRMRDL
                     LRSDKMTDHGVLENLCQEHASDFDLDSQNWMSFSDLLKKIPLSAGDQEDFLNSNRYFE
                     VQDSVSRYMVRIYDFKMRESVSPLSFEMENIRSVILNKRKFELLSRMEEDLFNDALKK
                     KKFEIY"
     gene            complement(3361..5358)
                     /locus_tag="PKI35_09010"
     CDS             complement(3361..5358)
                     /locus_tag="PKI35_09010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX44560.1"
                     /translation="MKRLALSLSIVVFILTPGFLYAQSQDNDPVILDVAGEKITRSEF
                     LNVYQKNNTKGEALDAKSLEEYLELYINFRLKVKEAQEQGLDTLRSFRDELAGYRKQL
                     STPYLVDDQAVESLVNEAYQRKLSDIRASHILVRCTREAAPADTLAAWNRVMQLRKRI
                     LKGEDFGKIAAENSDDESARNRTAQGRPVKGNYGDLGYFTVFDMVYPFETAAYNTKEG
                     EVSMPVRTDFGYHLIKVAARKPAMGKVQIAHILMIFPPKATAADSAKMADSIQMVYEL
                     LRNGGDFATLTKKYSGDKSTADKGGMLPWFGVNRMIPKFIDAIRDLKEIGDYSAPFMS
                     DFGWHIIKLADRKPVGSFEDEKGDLKQRVTKSDRTVVSQQSFIEKAKKEYSFTENKAA
                     LDKVISQVDPSVFAGKWSVPAQAALTETLFTIGNARYTQADLANYLASHQQKGDTLHI
                     PTYVDEQYKKFRDEKCQSYADSQLENKYPEFRSLMREYHDGILLFDLTDKKVWSKAIK
                     DTTGLQNFYEANKNNYLWDDRVDATLYTYANPKITKSLRKYVKKGLPFEQILPKFNHD
                     TVVVVEAERKKFLKGENATVDSLGWNKGFYPELPGKNGKLLTVVINDVVKKEPKQLNE
                     AKGIITADYQNYLEQEWIKELRKKYPFVANQEVLKSITENQ"
     gene            complement(5463..6932)
                     /gene="guaB"
                     /locus_tag="PKI35_09015"
     CDS             complement(5463..6932)
                     /gene="guaB"
                     /locus_tag="PKI35_09015"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNX44561.1"
                     /translation="MFPDDSKFVGEGLTYDDVLLLPAYSEVLPREADISTRFTRGIGM
                     NIPIVSAAMDTVTDSAMAIAMAQEGGIGVLHKNMPIEDQGAEVRRVKRAENGIIPDPV
                     TLHESALVADALEIMSVHKIGGIPVIDSSRKLVGIITNRDLRFERNPEKPVADVMTKK
                     SLITTTEFTDFEKAAGILQKYKIEKLPVVDKDFHLVGLITYKDIIKIKARPNACKDEL
                     GRLRVAAAVGVTSDTLERVSELVKNSVDAIVIDTAHGHSRGVIEMAKRVKRQFPDLQL
                     VIGNIATGEAALALMKAGADAVKVGIGPGSICTTRVIAGIGVPQLSAVFEVARALKGT
                     GIPVIADGGIRYTGDVVKALAAGASSIMAGSLFAGVDESPGETIIFEGRKFKTYRGMG
                     SIEAMQKGSKDRYFQDTEDDIKKLVPEGIVGRVPYKGTLSEVIQQMVGGLRAGMGYTG
                     SADINALQKARFIKITTAGVTESHPHDITITREAPNYSR"
     gene            complement(7106..8986)
                     /locus_tag="PKI35_09020"
     CDS             complement(7106..8986)
                     /locus_tag="PKI35_09020"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNX44562.1"
                     /translation="MKKIQLYLLAALTVLFSSGETAAQENAQGRNPQAIVLGITPVNA
                     GIARLDWVIQGMPSAGVFYVQRQYNTNWANIGTIDYDPANPVTTFSDTVSTPFCDSSL
                     INYRVAFIDDATVSLTISNVVSGNFLDINTPRNPSLDTVSIYYDPSGFYACPIIGWVK
                     SPQHDIAGYIIYRWNEVTQTFDSIQTAGPGSTTFTDLSINSLETCLNPHSYAIAAIDS
                     CQNKSYGTFMFAPHTISLQVMNDIDPCEKNVTLQWNGYDNMPDGLKGYQVFMETKDEN
                     QVIVQNFTLINEPGPSDTSYTDTRAFADGYSYTYFVRAVSNNGVSSSSSCRQIKTYHG
                     PVLPDTLYIEHATVVDDDYVNIKYYYSPENTIRKLVLERRIVPNGIFTVIDSLEMPAG
                     GFLPEVWEFNDTTAEVHYLSYQYRLLIQDTACVQAVDTSVNEARSILFLAWALDDNSN
                     QLVWNPYETWYGGVARYELFRSIDGVDDPANPIASLISDTLYVDNVTGISPTATICYR
                     IEAVEGTGNPVTSGERSVSNQACVIRDPSFFMPNAFRPSGILNRTFRPVASFVETANF
                     SMKIYNRWGQMIFETDNIFNGWDGNIGSVPAPAGVYAWLITYRSLLGKSYTKRGLVTL
                     IR"
     gene            complement(9042..10328)
                     /locus_tag="PKI35_09025"
     CDS             complement(9042..10328)
                     /locus_tag="PKI35_09025"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNX44563.1"
                     /translation="MNTIARYTQPPVNPLNRIQLARPEKYALHNGIPVYQINSGTQDL
                     VMIEMIFNAGSWYQPKPFTSLGTSLMLREGTKDHTSQEISEKLDFYGAHLETTTEKDL
                     ALVTLYTLNKHLDNTLPVLEDILKNAVFPDEECGIMLRKQKQALDIDMQKVRYLARTR
                     FNALLYGESHPYGAYRRQEDIEGASTELFRNFYHSHYQAANCRIIVAGKIPDSLPGQL
                     QRLFGGTDWMRPEINEPVFVPGPSGEKESFVVKDDALQSAIRMGRVMFNRNHPDFVGM
                     HFLNTLLGGYFGSRLMGNLREDKGYTYGIGSAIIPLQHSGFFFISAETGSEVTIKAVR
                     EIEKELEKLCNDLVSEDELSLVRNYLQGSFLRSIDGPFAMAERYRDLMQAGFDVEYFD
                     EMLETQRTITAQQLRELARKYLKPDEMYRLIAGKQA"
     gene            complement(10361..11596)
                     /locus_tag="PKI35_09030"
     CDS             complement(10361..11596)
                     /locus_tag="PKI35_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNX44564.1"
                     /translation="MIQFDRFTLLNGLRVIVHHDASTPIVAMNILYNVGSRDEDPNRT
                     GFAHLFEHLMFGGSVNIPRFDEPLERVGGENNAFTNCDFTNYYLTIPKPNLETAFWLE
                     SDRMLDLAFSAKSLDVQRNVVAEEFRQNYLNQPYGDTWLLLRPLAYKIHPYRWPTIGK
                     ELSHIENAGMDEVKTFYRKFYNPSNAILVVAGDVETEQIRELSEKWFGEIRKNGHYER
                     SLPVEPEQEEDRFLEVERKVPYDSICKVWHMCRRNNPSFYATDLISDVMSAGQSGRLY
                     AELVKKQQLFSELNAYITGDIDEGLFVISGKLIRGITFDQAEKAIESEIRKICSIPVE
                     ATELQKLKNRAETLMAFSEMRVLDKAMNLAYYEFLGDAALINRIIGLYNAVESRDIQT
                     EAMRVFRPGNCSTLHYKAV"
     gene            11762..14530
                     /locus_tag="PKI35_09035"
     CDS             11762..14530
                     /locus_tag="PKI35_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44565.1"
                     /translation="MKYLVIFFLSAVSVVAMAQGPAFILSAPAGDKYTAIDPSGTTVI
                     PNGRLVTPLGKCLTVAPHPYGLAIGKNGNMAVVANSGVKPLCITILRNLNSENPDIQQ
                     VPPGAANDKGVLASVFMGLAISPDEKLVYVAGGQENKIFIFDVETGEKTGEIDCSFSD
                     SKSDYTHGYIGDLVLSRDGRTIYAVDQVGFRMVVVDAVKRKLVANVPVGRYPFGICLS
                     PDEKQAFVANVGMYKYSLVRKKKNGEWKNGMIDYPAFAYGSEEAEEGILNDSLDIPGL
                     GPVDSDESFSVWAVDVGNPLKPRVTARIKTGTLVGQMVEGFPAVGGSSPNSLVSDGRY
                     VFCSNGNNDNVSVIDIRKKTVVKNIRLCPDERLSKLRGIIPFGLALSPDGKRLFVAES
                     GLNAIGVLDVKTLEVIGHIPAGWFPAKLKVTGDGKKLVVGNAKGFGSGPNGGAGFSKG
                     PEGTYIGCLMKGTVEVIDIPADDALPSLTQKVVDNNYMCRPADSKEFSWRKNNPVPLY
                     PGEKTSPIKHIVFISKENRTYDEIFGQVVKGSGDATLARYGANVSFTNNKKSRGVNFV
                     TVMPNHLNLAHTYAMSDNFYVDSDVSADGHRWLANTYPNEWVEASVPANYGGNRDYRE
                     NSKAPGNLGINGASGAIYPEDYNEAGSLWDHLARHNVGFFNFGFGVMFDPADYSEKYK
                     YMGIKYIANFPMPTPMYDRTSHMYPTFNMAIPDQFRIEMFTKEFNEKWGEGKAEMPQV
                     LTVILPNDHGAGERPDAGYPFRESYMADNDLAVGRIIEFLSHTPYWKNMAIIITEDDA
                     QNGVDHVDAHRSMLMVVSPYARKDYVSHVHYSFGSIFKTFWNILGIPYLNQYDAGASD
                     LADFFTDTPDFTPYRALPVDARIFDPQKALDPLDEKFDWKALEESPAIDNLPDMIRES
                     KEQEEWRK"
     gene            14659..15531
                     /gene="fabD"
                     /locus_tag="PKI35_09040"
     CDS             14659..15531
                     /gene="fabD"
                     /locus_tag="PKI35_09040"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNX44566.1"
                     /translation="MKAYVFPGQGAQFVGMGKDLYDKFPGAKEMFDKANAILGFNITD
                     LMFAGTDEDLRQTRVTQPAIFLHSVILAATLPDFRPDMVAGHSLGEFSALVANKALSF
                     EDGLRLVAARANAMQKACEAEPGTMAAILGLDDGTVERVLKGISEVVVPANYNSPGQL
                     VISGSMKGIELACEAMKAAGAKRALPLKVGGAFHSPLMEPARQQLAAAIETTSFNKPI
                     CPVYQNVNAKPCSDPAAIKANLIAQLTSPVRWTQTVQNMVADGAVLFIEAGPGTVLQG
                     LVKKIAPEVEAVSA"
     gene            15711..16598
                     /locus_tag="PKI35_09045"
     CDS             15711..16598
                     /locus_tag="PKI35_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44567.1"
                     /translation="MSASFKSMVSWRAVLTRELEEEQLREKEYLYQQVEFNDSGSPLC
                     EETYFADGILEHKYTYKYDENNRVVEEMLIEEGEFVSEHRTMDYNEAGLLNHEYRHYQ
                     DETCDTTEFVYDDKGKLLSKRTVDSDGEEESRTEYIYDKGLLILEKTDDSENDARSEK
                     HFSYDDSGHLLEESVNSPEEKFRHVNDYDEKGRRLISRRYNGDGHLVERVTFTYDDAD
                     NAVSYKEETTAGITFTALEYDAGGRIIMQEETSESGALISRILRRYDENGLLLENSVF
                     VEGSSRRAPQDYRVRYEYE"
BASE COUNT         4107 a         3919 c         4342 g         4622 t
ORIGIN      
        1 catcgggtcc aaacgcaaag aatacctttt tgagccgtac gctacatacg cggcggggtt
       61 gttcgggaag aagaaaatac ccaaagcgca gccgtagcag gtcttacttc aggagttcct
      121 gcacgatatg gtcttccttg atgccttcgg cttcggcttt gtaattgcgg acgatgcggt
      181 gacggaggat ggaattggca atggcctgca catcttcaat gtcgggggaa tatttgccat
      241 ttacggcagc atggcatttg gctcccagta ttaggtactg cgaggcgcgg gggccggcgc
      301 cccaggtaag ataatcgttg gcccaggcat gggcggcggg tgttcccgga cgggtgcgcg
      361 aagccagttt taccgcgtat tggaaaacat tttcggcaac cgggatgcgg cgtatcaggt
      421 cctggaaaaa gatgatttca tcgcgctgaa gaacgacttt caggtcggcc agatagttgg
      481 ccgtcgtggt tttcacgatc cggatttcct gttcgaacga agggtaatcg agccagatgt
      541 tgaacatgaa acggtcgagc tgggcttcgg gcagcggata ggttccttcc tgctcaatgg
      601 ggttttgggt ggccaaaaca aagaaaggtt cttcgagttt atggctcatg ccggcaaccg
      661 tcacggcttt ttcctgcatg gcttcgagca gcgcgctctg ggtcttggga ggtgtacggt
      721 tgatttcatc ggcgagaatg atgttggcaa acaccggtcc ccggatgaac ctgaactgcc
      781 ggttttcgtc caggatctca gtcccgataa tatcgctggg catcaaatcg ggtgtaaact
      841 ggatgcggct gtatgaaagt cccagcactt tgcttatcgt gtttaccatt aaggttttag
      901 ccaatcccgg cacgccgaca agcaagccat gccctttgct gaaaatggca atgatcagat
      961 tctttatcgc ttcatcctgg ccaatgatga ctttaccgat ctcattacgc agttcattgt
     1021 atttcagtgt gaaagcatca agtgcttcgg tatcggactt aaaagaaatc atagcttata
     1081 aagtattact caacgttggt atgcggcata gggtgacaaa cggccccctg ttttaaggat
     1141 gatttgcctg ttacaggttc cagttatact ggaaaggaca attcttgtat ttatcgtcaa
     1201 tcctgatgaa cgcagtcttt gatttgtcag cgatccattt tgtcacggct ttcccttttt
     1261 tctcgttgag cgcccattcc tgtatctggt cgtaatcatc tttcagggtt gccttgtgtg
     1321 gttgtgtacg tgaaacaagt ttcaggatac ggtaggctga agttccgtcc tcggttgaat
     1381 agggaacggc agccgaaacg cctcctacat ccagtttgtc gattacgaaa aatattttgg
     1441 gatcaagctg gtcggcaggg aagacggagt tgttcgatgc ctgattgata agaagtccgt
     1501 tatttacttt ccccggatcg tcgctgaatt tcaacgcggc cttctcgaag gaaatactgt
     1561 cttttgcaat taatccggca atactgtcga gttcgttttt ggcccgcgaa aggtcaaggg
     1621 gcgaaacttt aggaatgacg aggatgtgcc gggcattgat cagttctccg cgcctctcga
     1681 taagctggat gatatggaag ccgaattttg tccggataac ctccgacata tcatccttgt
     1741 ttttcaggct gaaagccgcc gcttcaaatt caggggccat atcgccacgt ccgaaaaaac
     1801 ccagttcgcc ccctttggag gctgatcccg ggtcctcgga atacaggacg gccagcgtgc
     1861 tgaacttctc tcccttgagg atacggtcgc gcaaggctgt aattttttca cgggatgcat
     1921 tcagttcttc cgaacttaca tcgggttctt taacgatctg ggcaatttcg tatttcatcg
     1981 ggatcaccgg aatactgtcg gcgggaattt tctcgaagaa tttttttact tcggtgggag
     2041 tgatcttaat attctcggtt atttttctct gcacctgttc cgacagcatc tgctcgcgga
     2101 tcggttcgcg gaattcttct tttatctcca ggattgattt atcataaaag tcttccagct
     2161 tttcctgcga tccgaattgc tggatataat agcgcagttt ctgatcgagc gtggcgttta
     2221 cctgttcgtc atcaaccaca atactatcca cttcagcctg gtagagcagc aatttctgaa
     2281 aaagcagttg ttcaaatatc tggcaacggg cagccgcagg gtcgggtact gttgtttggg
     2341 ctttcatctg catatactga acttccacgt cggagtaaag aacaggatga cttcccacga
     2401 cggcaaccac ctcatcaatc actttatcct gtgctttcac aggtatggtt gtgaagatgg
     2461 ggatcaggaa cagggctgta aagaagaatt ttttcattag cgaagcggga gattaataaa
     2521 tttcgaattt cttttttttg agcgcgtcgt taaagaggtc ttcttccatg cgcgacaata
     2581 gttcaaattt tctcttattc aggatgacag aacggatatt ctccatctcg aaactaaggg
     2641 gcgatacgct ttccctcatc ttaaaatcat agatgcgcac catataacgg cttaccgaat
     2701 cctggacttc aaaatacctg ttcgagttta gaaaatcttc ctggtcaccg gctgataagg
     2761 ggattttttt caataagtcg ctgaacgaca tccagttttg ggaatcaagg tcaaaatcag
     2821 aggcatgttc ctggcagagg ttctcgagga cgccatggtc ggtcatttta tcacttcgaa
     2881 gcagatcccg catccggggt atttcacgcg ttttcagggg agcaatgatg tagcatgctt
     2941 ttacgatgtt ttcccttaac tggaaatttg cctggttttt gttgtagtaa tctacgattt
     3001 ccttctcgga aatcaccgta tcgagctttt gttttacgag ttccgattca tactcgtaca
     3061 taatcaacga attgcggtac tgctccagtt ccttcgtaaa atcggactta tcgctgctca
     3121 ggttgttttc ggcctgttgc agcagcaact gttggcgaat ccagttgttg atgtaggttt
     3181 tcaccagatc ggcgctgtct ttttcatccg ttcctgccgg aacgacatca gccaggtcgt
     3241 cgcggtacaa ataggtatcg ttgacgcggg cgacagcctc ttttttactg ccatggcctg
     3301 aaaacaggct gcatgaagaa gccagcagca caatcggtag gaaaagaaac agttttggca
     3361 ctattgattt tcggttatgg atttgagcac ttcctggttt gcaacaaagg ggtatttctt
     3421 acgcaattcc ttgatccatt cctgttccag gtagttctga taatcggcgg tgataatacc
     3481 tttagcttca ttgagctgtt tcggttcttt tttaaccaca tcattgatga ccacagtcag
     3541 tagtttgccg tttttaccgg gcagttccgg ataaaatcct ttattccatc ccaggctgtc
     3601 gaccgtcgca ttttcacctt tcaggaactt tttcctttca gcctcaacaa ccacgaccgt
     3661 atcatggttg aatttcggca ggatctgttc gaaggggagg cctttcttca catatttcct
     3721 gagcgatttg gtgattttcg ggttggcata agtgtacagg gtggcatcaa cgcggtcgtc
     3781 ccagaggtag ttgttcttat tggcttcata gaagttctgc aggccggtag tgtcttttat
     3841 agcttttgac catacctttt tgtccgtcag gtcgaaaagg aggattccgt cgtgatactc
     3901 gcgcatgagt gagcggaatt caggatactt gttctcgagc tggctgtcgg catacgactg
     3961 gcatttttcg tcgcggaact tcttgtattg ttcatcaacg taagtaggta tgtgcagcgt
     4021 atctcctttt tgctggtgcg atgcaaggta atttgccagg tcggcctggg tgtaacgggc
     4081 attaccgatt gtaaacaggg tttcggttag ggcggcctga gccggaacag accatttgcc
     4141 tgcaaatacg gaaggatcaa cctgggaaat cactttatca agcgcagcct tgttctcggt
     4201 gaaactatac tcttttttag ctttttcaat gaaggattgt tgcgatacga cggtgcggtc
     4261 gctcttggtt acccgttgtt tcaggtcgcc tttttcgtct tcgaacgaac ctaccggttt
     4321 gcggtcagcc agtttgatga tatgccagcc gaaatccgac atgaaaggcg ccgaataatc
     4381 cccgatttct ttcaggtcgc gaatggcatc gatgaatttg gggatcatac ggttaacgcc
     4441 gaaccagggc agcatgccgc ctttgtcggc agtgctcttg tcgccggagt attttttggt
     4501 cagggttgca aaatcaccgc cgttcctgag cagttcatat accatttgga ttgaatcggc
     4561 catctttgcc gagtcggcag ccgtcgcttt tggcgggaag atcatcagga tatgggcaat
     4621 ctgtactttt cccatagccg gtttcctggc tgcaaccttg atgagatgat agccgaaatc
     4681 ggtgcgcacc ggcatcgaca cttcaccttc ttttgtgttg taggcagcgg tttcgaaagg
     4741 ataaaccatg tcgaaaacgg taaaataccc caggtcgccg tagttgcctt tgacggggcg
     4801 gccctgagcg gtccggttcc tggctgattc gtcgtccgaa ttttcagcag caatttttcc
     4861 gaagtcttcg cctttgagga tccgtttccg gagttgcatg acgcggttcc aggccgccag
     4921 cgtatcggca ggggcggctt cccgtgtgca ccgcaccaga atgtggcttg cgcggatgtc
     4981 ggataatttg cgctggtaag cttcgtttac cagtgattca actgcctggt catccaccag
     5041 gtaaggagtg gataattgtt ttcggtatcc ggcaagttca tcacgaaacg aacgaagggt
     5101 atcgagtccc tgttcctgtg cctcttttac cttaaggcgg aagttgatat agagttcaag
     5161 atattcctcc agcgatttgg catcaagggc ttcccccttg gtattattct tttggtaaac
     5221 attcaaaaac tcgctacgtg taattttctc accggcaaca tctaaaatca cggggtcatt
     5281 atcctgggat tgggcgtaaa ggaaacctgg agtcaggata aaaacaacga tggaaaggct
     5341 cagggccaaa cgtttcataa gttttgtgtt agtaattaat aatcggtagg ttaagaggat
     5401 agtaagtttg ttttttattt atggttgatt cccgggtcag aaacgcgggg aaattttttt
     5461 tattacctgc tgtagttggg agcttcccgg gttatggtga tatcgtgagg atggctttcg
     5521 gtaacgccgg cagttgtgat tttgatgaac ctggcttttt gcagggcgtt gatatcggcc
     5581 gatccggtat atcccattcc ggcgcgcaac ccgccaacca tttgttgaat tacttccgag
     5641 agtgtgcctt tgtatggaac cctacccacg atgccttcgg ggaccagttt ttttatatcg
     5701 tcttcagtat cctggaaata gcggtctttc gaacctttct gcattgcttc gatggagccc
     5761 atgccccggt atgtcttgaa tttgcggcct tcaaaaatga tggtttctcc cggcgattcg
     5821 tcaacgccgg caaagagcga acctgccata atactgcttg ccccggctgc caatgctttg
     5881 accacatcgc ccgtgtaccg gataccgcca tcggcaatta caggaatacc agtgcctttg
     5941 agcgccctgg ccacctcaaa cacggctgaa agttgcggaa ctccgatgcc tgctattacg
     6001 cgggtggtgc agatagatcc cgggccgata ccgaccttta ccgcatcggc gccggctttc
     6061 atcagggcga gggctgcttc gccggtagca atattgccga taacaagctg cagatcgggg
     6121 aactgtcgtt tcaccctttt tgccatttcg atgacgcccc gggaatgccc gtgggcagtg
     6181 tcgataacga tggcgtcaac ggaatttttt accagttcgc tcacgcgttc cagggtatcg
     6241 gaagtaacgc ctacggctgc ggcaacgcgg agtcgtccca gctcatcttt acaggcgtta
     6301 ggacgggctt ttatcttgat gatgtccttg taagtgataa gtcctaccag gtgaaaatcc
     6361 ttgtctacaa cgggaagttt ctcaatcttg tacttctgga ggattccggc ggctttttcg
     6421 aagtctgtaa actcggtggt agtgataagg cttttctttg tcattacatc ggcaacaggc
     6481 ttttcggggt tgcgctcgaa gcggagatcg cggttggtga taattcccac aagttttcgg
     6541 ctgctatcga taaccggaat acccccgatt ttatgcacac tcattatctc cagcgcatct
     6601 gcaaccaggg cgctttcatg taaggtgacc gggtcgggaa ttatgccgtt ttctgcgcgt
     6661 ttaaccctcc tgacctccgc gccctggtct tcgataggca tgttcttatg gagtacgccg
     6721 ataccgcctt cctgggccat ggcaatagcc atcgccgaat cggtaaccgt atccattgcc
     6781 gctgaaacta taggtatgtt catcccgatt ccccgtgtaa accgggtgga gatatcggct
     6841 tcgcggggaa gcacttcgga atacgccggc aacagtaata cgtcgtcgta cgtaagccct
     6901 tcacctacga attttgaatc atcaggaaac atggcctttt tttgaaaggt gcaaagttat
     6961 taattttagt aaaagtgctt acttaaaatt tgttaaggaa ttctttgaaa gtaacaggat
     7021 tataacttga tttcagaaca ttatcagggg ctgttttaat agcttgaatt caaaagatac
     7081 cctgatggat catttcagga tattgttacc ggatcagggt gacaagtccc cgctttgtat
     7141 aagattttcc gagcaacgaa cggtacgtga tcagccaggc atatacgccg gcaggagcag
     7201 gaacgctgcc aatattaccg tcccacccgt tgaaaatatt gtcggtttca aatatcatct
     7261 gaccccagcg gttataaatc ttcatgctga aattagccgt ctctacaaac gatgccacag
     7321 gcctgaaggt acggtttaaa atgccgcttg gcctgaaagc attgggcatg aaaaacgacg
     7381 ggtcacggat aacgcaggcc tggtttgaga cagaccgttc tccggaagtt acagggttcc
     7441 ccgtaccttc aacggcttca atacggtaac atatggtagc tgtcgggctg ataccggtga
     7501 cattatccac atacagggtg tccgaaatca gcgaagcaat tgggttggcc ggatcgtcca
     7561 caccgtcaat actcctgaac aattcatacc gcgctacacc gccataccag gtttcataag
     7621 gattccagac caactggttt gaattgtcat cgagggccca ggctagaaac aatatcgaac
     7681 gtgcctcgtt aaccgaagta tcaacagctt gcacacatgc cgtgtcctgt atcagcaacc
     7741 ggtattgata gctaaggtaa tggacttcgg cagtggtgtc gttaaattcc cacacctcag
     7801 gcagaaaacc gccggccggc atttccaggg agtcaatgac cgtaaatatt ccgttcggga
     7861 cgatccttcg ctcaagaacc agtttccgga ttgtgttttc cggcgaataa taatacttta
     7921 tattgacata gtcatcgtct accaccgtgg cgtgttcgat gtaaagggtg tcgggaagca
     7981 cagggccatg atatgttttt atctgccggc acgaggaact gcttgagacg ccgttgttgc
     8041 tgaccgcccg tacgaaatag gtgtaagaat agccgtcggc aaaggcccgt gtatcggtat
     8101 agcttgtatc cgaagggccg ggttcattta tcagggtgaa attctgaaca attacctgat
     8161 tttcatcctt ggtttccata aaaacctggt atcctttaag gccgtcaggc atattatcgt
     8221 atccgttcca ctgaagcgta acatttttct cacagggatc tatatcgttc attacctgca
     8281 gtgagatcgt atgaggcgca aacatgaaag tgccgtaact cttgttctgg caactgtcga
     8341 tggcggcaat tgcgtatgag tgcgggttca gacaggtttc gagggagtta atgctcaaat
     8401 ccgtaaacgt cgttgaacca gggcctgccg tttgaataga gtcgaacgtc tgagtgactt
     8461 cattccaacg gtatatgata taaccggcaa tatcatgctg tgggcttttt acccagccga
     8521 tgatgggaca ggcataaaac ccggaaggat cgtaatagat gctgacggta tcgagagatg
     8581 gattccgggg cgtattgata tctagaaaat ttccggatac gacatttgaa atcgtaaggc
     8641 tgacggttgc atcgtcgata aatgcgaccc gataattgat cagtgaactg tcgcagaatg
     8701 gtgttgagac tgtatcagaa aatgtggtaa caggattggc cggatcgtaa tcaatggtgc
     8761 caatatttgc ccagttggta ttatattgcc gttgaacata aaagacgccg gcactgggca
     8821 tgccttgaat gacccagtcc agccgggcta tacctgcgtt aacaggggtg atgccgagga
     8881 cgatagcctg agggtttctg ccttgtgcat tttcctgcgc tgcggtttca ccggatgaga
     8941 acagaacagt aagagccgcc aggagataaa gctgtatttt tttcatcagc gtagcccaat
     9001 attgaaaatt cagacgttaa atgtacgaat tatagaatct ctcaggcttg ttttccggca
     9061 atgagccggt acatttcatc cggtttcagg tatttccggg ccagttcgcg gagttgttga
     9121 gcggtgatgg ttcgttgcgt ttcgagcatt tcatcgaagt actcaacatc gaaccctgcc
     9181 tgcatcaggt cgcggtatct ttcggccatg gcaaaggggc cgtcgatact cctgaggaat
     9241 gagccctgga ggtaattccg caccagcgac aattcatctt cactcaccag atcattgcaa
     9301 agcttctcca gttccttttc aatttcccgg acggctttta tggtcacttc gctgcctgtt
     9361 tcggccgaga taaagaaaaa gccggaatgt tgcaggggaa ttatggctga accgatgcca
     9421 taggtatatc ctttatcttc gcgcaggttt cccatcagcc ttgaaccgaa atagccgccc
     9481 agcaaggtgt taaggaagtg catgcccaca aaatcgggat ggttcctgtt aaacatcacc
     9541 ctccccatac gtatagccga ttgcaaggcg tcatctttta ctacgaaaga ttccttttct
     9601 ccggacggac cgggtacaaa aaccggttcg ttgatttcag gtctcatcca gtcggtaccg
     9661 ccgaataaac gctgaagctg acccggcagg ctgtccggaa ttttaccggc aactatgatc
     9721 ctgcaattgg ctgcctggta atgcgaatga taaaagtttc tgaaaagttc agtagaggcc
     9781 ccctcaatat cttcctgccg gcggtaagct ccataaggat ggctttcacc atacagcaat
     9841 gcattgaagc gggtacgcgc caggtaccgc actttttgca tgtcaatatc aagggcctgt
     9901 ttttgtttcc tgagcatgat gccgcattct tcatcaggga aaacggcgtt tttcaggata
     9961 tcttccagta ccggaagcgt attatcaagg tgtttgttca gcgtataaag ggtaaccagg
    10021 gcaaggtcct tttcggttgt cgtttcgagg tgggccccgt agaaatccag cttttccgat
    10081 atctcctgcg atgtatgatc ctttgtccct tcccgcagca tcaggctggt tcccaatgat
    10141 gtaaaaggtt tcggctggta ccagctcccg gcattgaaga tcatttcgat cattaccagg
    10201 tcctgggttc cggaattaat ctgatacacc ggtattccgt tgtgaagggc gtatttttca
    10261 ggacgggcaa gctgtatccg gtttaaagga ttgaccggtg gttgagtata tcgtgctatc
    10321 gtgttcatcg gcgttttttt ggagtgttta tcccaatgga tcagacggcc ttgtagtgca
    10381 acgtggaaca attgccgggc cggaaaaccc gcattgcttc cgtttgaata tcccggcttt
    10441 caacggcatt gtacagtccg atgatccggt tgatcagcgc ggcgtcgcca agaaattcgt
    10501 aataggccag gttcatggcc ttgtcgagaa cgcgcatttc cgaaaaagcc atcagggttt
    10561 cggcacggtt cttcagcttt tgtaattcag ttgcttcgac aggaatgctg catattttcc
    10621 tgatttcgga ttcaatggcc ttttcagcct ggtcaaaggt gattcccctg atcagtttcc
    10681 cggaaattac aaacaaacct tcgtcgatat cgccggttat gtatgcattc aactcgctga
    10741 aaagctgctg ttttttaacc agttcggcat agagcctgcc tgattgtcct gccgacatta
    10801 catcgctgat caggtctgtg gcataaaagg aaggattatt tctccggcac atatgccaga
    10861 ccttacagat ggaatcatag ggcacttttc gttccacctc caggaagcgg tcttcttctt
    10921 gttccggttc caccggaaga gacctttcat aatgtccgtt tttcctgatt tccccgaacc
    10981 atttttccga caattccctg atctgttctg tttcaacatc tcccgccaca accaaaatgg
    11041 cattcgaagg attgtaaaat ttccggtaga atgtttttac ctcgtccatg cctgcatttt
    11101 caatatgcga tagctctttc ccgatggtag gccagcggta aggatgaatc ttgtaagcca
    11161 gcgggcgcag caacagccac gtatcaccgt aaggctgatt aaggtagttt tgccggaatt
    11221 cttcggctac aacgtttcgt tgaacatcaa ggcttttagc agagaaagcc aggtcgagca
    11281 tacggtccga ttcgagccag aatgcggttt ccaggtttgg ttttggaatt gtaaggtagt
    11341 aatttgtgaa gtcgcagttg gtgaaggcgt tgttttcgcc gccaacacgt tcgagcggct
    11401 cgtcaaaacg cggaatgttg acgctgccgc caaacatcag gtgctcaaac aaatgggcaa
    11461 agccggtacg gttgggatct tcgtcgcgcg aacctacatt gtaaaggatg ttcatagcca
    11521 caattggcgt agaagcatcg tgatggacaa tcacccggag cccattaaga agtgtaaacc
    11581 ggtcaaactg gatcataaaa atctgctata gttcttaaaa cggagaaaca ggatattccg
    11641 tataattcac ggcaaaagta ggtcattttt ctgaggcaaa gggagggctg aacttttgtt
    11701 tgcgcccgat tcctcaatgg tttcctcaat aatcttacat ttgtttactg acatcatgct
    11761 catgaaatac ttggtaatct tttttctttc agccgtttcg gttgtggcga tggcacaggg
    11821 tccggcattt attctttcgg cccccgccgg tgataagtac actgctattg atccctcggg
    11881 tactaccgtg atccccaacg gccgccttgt aacacccttg ggcaagtgcc tgacagtagc
    11941 accgcatcca tacgggctgg ctatcggtaa gaatgggaat atggctgttg tcgccaattc
    12001 gggcgtcaag ccgttatgta taaccatcct gcgcaacctg aattcggaaa atcccgacat
    12061 acagcaggtt cctcccggag cggccaacga caagggcgtt cttgcttcgg tcttcatggg
    12121 acttgcaatt tctcccgatg aaaaactggt ttatgttgcc ggaggacagg aaaataaaat
    12181 cttcattttc gatgtggaaa ctggtgaaaa aaccggagaa atcgattgca gcttttcgga
    12241 ttcaaaatcg gattataccc acggttatat cggcgatctg gtgctgagcc gcgacggcag
    12301 aaccatctat gccgtcgatc aggttggttt ccgtatggtg gtggtcgatg cggtgaaacg
    12361 gaagctcgtg gccaacgttc ctgtcggcag gtaccctttc gggatatgcc tttcgcccga
    12421 tgaaaagcag gcttttgtcg ccaatgtggg aatgtataaa tacagccttg tcaggaagaa
    12481 aaagaacggg gaatggaaga acgggatgat cgactacccg gcttttgcct atggttcgga
    12541 ggaggctgaa gaagggatat taaacgattc cctcgatatt ccggggctgg ggcctgttga
    12601 ttcggacgaa tcgttttccg tctgggctgt tgatgtagga aatcctttga aaccacgggt
    12661 aacggccagg attaaaaccg gcacgctggt tgggcagatg gtggaaggtt tcccggcggt
    12721 gggaggatcg agccctaatt ccctggtatc ggacggacgg tatgtttttt gcagcaacgg
    12781 caataatgac aatgtcagcg ttatcgacat acggaagaaa accgtcgtta aaaatatcag
    12841 gttatgtccc gatgaaaggc tcagcaaatt gcgcggaatc attcctttcg ggctggctct
    12901 ttcgcccgat ggtaaaaggc tgtttgtggc ggagtcgggt ctgaatgcca tcggcgtgtt
    12961 ggatgtaaaa acgctggaag tcatcgggca tattcctgcc ggctggttcc ctgccaaact
    13021 gaaagtaacc ggcgacggta aaaaactggt ggtaggcaat gccaaaggct ttggcagcgg
    13081 cccgaacggg ggcgctggat tttcgaaagg gcccgaaggg acctatatcg gttgcctgat
    13141 gaaaggaacg gtggaagtga tcgatattcc ggctgacgac gccttaccat cgttgaccca
    13201 aaaagtggtt gacaataatt acatgtgtcg ccctgccgac agtaaagaat tctcctggcg
    13261 gaagaataat cccgtgccgt tgtatccggg agaaaaaaca tccccgatca aacatattgt
    13321 cttcatctcg aaggaaaacc gcacttatga tgaaattttc gggcaggtcg tgaaaggcag
    13381 cggcgatgcc acactggcaa ggtatggtgc caacgtttcg ttcaccaata acaaaaaaag
    13441 ccggggcgtg aacttcgtca cggtgatgcc caaccacctg aatctggccc atacctatgc
    13501 catgtccgat aacttttatg tcgacagtga tgtttcggcc gacggacacc gctggctggc
    13561 aaacacctat cccaacgaat gggtggaagc cagtgtgccg gccaattacg gtggtaaccg
    13621 cgattaccgg gaaaacagca aagcgcccgg gaacctcggc atcaacggag cttccggcgc
    13681 catataccct gaagattaca atgaggcagg ctcattatgg gaccatctgg cccggcataa
    13741 tgtcggcttc ttcaattttg gtttcggggt gatgtttgat ccggccgatt acagtgaaaa
    13801 atacaaatac atgggtatca agtatattgc caattttcct atgccaacac ccatgtacga
    13861 ccgcacttca cacatgtatc ctactttcaa catggccatt cccgatcagt tccggatcga
    13921 gatgtttacg aaggaattta atgagaaatg gggtgaaggg aaagctgaaa tgccgcaggt
    13981 gctaacggta attctgccga atgaccatgg cgccggcgag agaccggatg cgggttatcc
    14041 tttccgcgaa agctatatgg ccgataacga cctggcggtg ggacgtatca tcgagttcct
    14101 ttcgcatacg ccttactgga aaaatatggc cattatcatc accgaggacg acgcccagaa
    14161 cggtgtagat catgtggatg cccaccggag catgctgatg gtagtttcac catacgccag
    14221 aaaagattat gtgagccatg tacattatag tttcggtagt attttcaaaa ccttctggaa
    14281 tattctcggc attccttacc tgaaccaata tgacgccggc gccagcgatc tggcggattt
    14341 ctttacggat acccccgatt tcacgcctta cagggcattg ccggttgatg cacgcatctt
    14401 tgatcctcag aaagccctcg atccgctgga tgaaaaattc gactggaaag cgctggagga
    14461 aagcccagcc atcgacaacc tcccggatat gattcgcgaa agcaaggaac aggaagagtg
    14521 gaggaagtag ggcgggtaac tgcccggtaa aagaagttgc ggggccggaa ggtttgaaaa
    14581 tctgagattc cggctcccgg atcgaaaagc tggttttagg ctttttatat atttgccgtc
    14641 cattttaacc caacaactat gaaagcatat gtatttcccg ggcaaggcgc ccagtttgtc
    14701 ggaatgggaa aagatttata tgataaattc cccggagcca aagaaatgtt cgataaggca
    14761 aatgccatac tcggattcaa tattactgat ctgatgtttg ccggtaccga cgaagacctc
    14821 cgtcagacca gggttacgca accggccatt ttccttcatt cggtaatatt agctgcaacc
    14881 ttgcccgatt ttagaccgga tatggtagcg ggacattcgt tgggcgaatt ttcagcattg
    14941 gtagccaaca aagcattatc gttcgaggat ggtttacgac tggtggctgc aagggctaat
    15001 gccatgcaaa aggcttgcga agccgaacct ggcactatgg ccgccatcct gggccttgat
    15061 gatggaaccg ttgagcgcgt tctcaaagga attagcgaag tggtggttcc ggccaactat
    15121 aacagtcccg gtcagcttgt gatctccggt tcgatgaaag gcattgaact tgcctgtgaa
    15181 gccatgaagg cagcaggcgc caaacgcgct ctgccgctta aagtcggcgg cgccttccac
    15241 tcgccgttga tggaaccggc acgacagcaa ctggctgccg cgattgaaac gacctccttc
    15301 aacaagccca tttgcccggt gtatcagaat gttaatgcaa aaccttgttc cgatcccgcc
    15361 gctattaagg ccaacctgat agcacaactc acttcccctg tccgatggac ccagacggtt
    15421 cagaacatgg ttgccgatgg cgcagttttg tttattgaag caggtcccgg aacggtactg
    15481 cagggattgg tgaagaaaat agccccggag gtcgaggcag tatcagcctg aattccggtt
    15541 atccgctaag ctttattaaa ggcaggcccg gtcacattgt cttgtcagat gccggttatg
    15601 cctgagtcaa ccggcgaaat gtgcctgagg cataatctaa accgcataaa ggtataccgg
    15661 ttgaaggtga attttatagt tttgcatcca ttaattctta ccctagcact atgtcagcat
    15721 cattcaaatc aatggtttca tggagggctg tgctcacccg ggaacttgag gaagaacaac
    15781 tccgtgagaa ggaatatctc tatcagcagg ttgaatttaa cgattccggt tcaccgttat
    15841 gtgaggaaac ttattttgcc gatgggatat tggagcataa atacacttat aaatatgatg
    15901 aaaataaccg tgtagtcgaa gaaatgctga ttgaggaggg tgaatttgtt tcggaacacc
    15961 ggactatgga ttataatgaa gccggacttc tgaaccatga ataccgtcat tatcaggatg
    16021 aaacatgcga tacaactgaa ttcgtctatg atgataaagg aaaattgttg tcgaaaagaa
    16081 ctgtcgatag cgacggggag gaggaaagcc ggactgaata catttatgat aaaggcctcc
    16141 tgatacttga aaaaaccgac gattcggaaa atgatgccag gtctgagaaa catttttcct
    16201 atgatgattc cgggcatctg ctcgaagaat cagtaaattc tcccgaagaa aagttcaggc
    16261 acgtgaatga ttatgatgaa aaaggcaggc gcttaatctc caggcgttat aatggcgacg
    16321 ggcaccttgt ggaaagagtc acatttacat acgatgatgc ggataatgct gtttcctata
    16381 aggaagaaac aacggccgga ataaccttta cagcgctgga atacgatgcc ggcggacgca
    16441 ttatcatgca ggaggaaact tctgagtcgg gagctcttat cagcaggatt cttcgtcgat
    16501 atgatgaaaa cggcctgttg ctggagaatt ctgtttttgt tgaaggctca tcccggcggg
    16561 cgcctcagga ttacagggta aggtatgagt atgaataagt gataagtgat tagtgattat
    16621 tgattcgtta ttcgtgcaat tcgtgtctcg cgggcagtgt tccagtgttt cagacctgcc
    16681 tgccttgtgc agggctgtgg gaagaggcag ggtttcagcg tttcaaggtt tgggtttaag
    16741 cctttgcgtt ctgcgttctg cgttttgtgt tttgagtttt gagtggaatt cgtggttcgt
    16801 ggaattcatg gttaggtttt atccacgaat tagcacgaat gaagcacgaa tgaacactga
    16861 taattcgtgt cccggcgaag ccgcttttcg gataaatagt gattcgctaa tagtgattcg
    16921 tggaattcgt gatagcattc gtgaaattcg tggttcgtga ttagtaatta gtaattcgtt
    16981 gttcgcaaat
//
LOCUS       DAOPYV010000067        16537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_3710_length_16537_cov_12.580876, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000067 DAOPYV010000000
VERSION     DAOPYV010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16537
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3710_length_16537_cov_12.580876"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /gene="ftsH"
                     /locus_tag="PKI35_09050"
     CDS             complement(<1..1472)
                     /gene="ftsH"
                     /locus_tag="PKI35_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNX44568.1"
                     /translation="MAEETPNSDNNRKPFNGNLPKLKNPRFKFNFYWIYGILAVIFTF
                     LYFSNMGTSPKTIGWGELKGMLVSKDVEKIILVNKEQAEIFIKKDRLSLEKYKDLKQG
                     NSLTSSTPHYIYQIVSAESFEKEIQEAQKDNPTPVYIQVVTRKNWGMDVLGWILPFVI
                     LIGGWFLIMRMMSKGGGGGGGQIFNIGKSKAQLFDRDTMVTTNFNDVAGLEEAKVEIM
                     EIVEFLKNPKKYTELGGKIPKGALLVGPPGTGKTLLAKAVAGEAKVPFFSLSGSDFVE
                     MFVGVGASRVRDLFKQAKDKAPCIIFIDEIDAIGRARGRNPMTGSNDERENTLNQLLT
                     EMDGFQTNAGVIILAATNRADILDRALLRAGRFDRQIHVELPDLEERKAIFKVHMRNL
                     KLDETVNVDFLARQTPGFSGADIANVCNESALIAARNDKKQIDKQDFLDAIDRIVGGL
                     EKRNKIISPHEKKVIAYHESGHAAISWLVEHANPLVKVTIV"
     gene            complement(1502..1870)
                     /gene="rsfS"
                     /locus_tag="PKI35_09055"
     CDS             complement(1502..1870)
                     /gene="rsfS"
                     /locus_tag="PKI35_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNX44569.1"
                     /translation="MAKHAKSEASDMLADIVVHGMAERKAQEIVKLNLGQLQNSITDY
                     FVICHGTSRTQVEAIADSVEAEVKKAVGLSPWHREGFENCEWILLDYFDVVVHIFQAE
                     TRSFYQLERLWADAGRTNVS"
     gene            2057..2806
                     /locus_tag="PKI35_09060"
     CDS             2057..2806
                     /locus_tag="PKI35_09060"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNX44570.1"
                     /translation="MRIIGSSIISLDESASTNDYALELLKSSKPADGTVVTTKKQSAG
                     RGQENNSWESEPGKNLTLSIILYPHFLPPDRQSLLSMAIALGVYDLINSHITAQPVRI
                     KWPNDIYIGDRKACGMLIQNSIMGNRFDYAIAGIGLNVNQEKFISDAPNPVSLKMIAG
                     REFPTEELLWQLCGWLDTRYHQLRLGDRKGISEEYLSLMYRYNEWHVFEIRHRAAEAR
                     ITGINRYGQLQLEERNGTRSECDMKEVKFLI"
     gene            complement(2808..4712)
                     /locus_tag="PKI35_09065"
     CDS             complement(2808..4712)
                     /locus_tag="PKI35_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNX44571.1"
                     /translation="MTFNLFVIPFFGGMLFLLAVIIIRFTAWLATLDRSGRKKLGHGL
                     LNGQIFPATWEVFMESLLHRRMFRQNKLLGFMHMSFAFGWFLLIVMGNLESRIYSGNH
                     LNPPYYPIFLKFFVHDKRALPFELYTLPGFFRFIMDFLLLFILSGLVLAFIKRTRSKW
                     FGMKRTTQHNKLDKWAITSLWLIFPMRLLAESFTAGVYQGGGFLTNTLGMAFASFLPV
                     EHLYYPAWWAYSIALGVFFVTLPWSRYMHIPTEVLLIYLRHFGIKTGKKPDSFTDIEI
                     HSCPRCGVCIDVCQMNYAGKTDATPAYFLRSLRYGKQNAIQDANCMLCGRCHQVCPVQ
                     IDTVGIRMASRYIDNVLVSPSYNYLDSIQIPEPRVDMLYFAGCMGHLTPSVKKAMVKI
                     LDASGENFSFMDEDGSVCCGRPLMMAGLPEAAGELIRKNTETIVKSGAKTFVTSCPIC
                     FKVFSEEYKLPGIEVLHHSQYLLRLVESGKIKVDASPVLAVYHDPCELGRGSGIFEEP
                     RRLLNKMVCLADISQQREHALCCGGSVGDLAMNYNERTVIRDEALKILLEPNPDLLVT
                     ACPLCKKTFDNGSDVMVMDLAEIVSERMPESSDSTLFEAPVTQSRQQASLLHEAGKEV
                     KFPVCNPELR"
     gene            complement(4709..5062)
                     /locus_tag="PKI35_09070"
     CDS             complement(4709..5062)
                     /locus_tag="PKI35_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNX44572.1"
                     /translation="MSDIKEKVRFGFTVNKDRQIDYDANSKKLASTVIIAEPSLNLCI
                     SCGTCAATCTAAQFTDFSLRRLILLVRRGEVSGLENEVTKCMLCGKCQLVCPRGVNTR
                     NLILQIHKALKNTVL"
     gene            complement(5090..6106)
                     /locus_tag="PKI35_09075"
     CDS             complement(5090..6106)
                     /locus_tag="PKI35_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX44573.1"
                     /translation="MSRHVAVIGAGAAGLEASSALASMGFRVTLVEKEDSTGGHVKDW
                     HALFPNRRPGAEVLDSLRKNLDPGVNLVTGKPVSAIKPNGKTFFVESGNDWQTEADAV
                     LMATGFSLFDARKKEEYGYRIYDNVITSADLEAAFSAGKVTMKNGEIPKRIGFVHCVG
                     SRDEKAGNIYCSKVCCVTAVKQAIEIKEFLPGTEVFCFYMDLRMFGRHYEELYKEAQE
                     KYNVQFIRGRLSESTEDHNGKVILKVEDTLLGKPLKIGVDMLVLMAGMESPSGTRQLR
                     DMLHLAKDNDNFLIPSDAHLSANISEIPGLFYAGTCTGPKNIADTLADARSAALAVAK
                     YLKS"
     gene            complement(6103..7179)
                     /locus_tag="PKI35_09080"
     CDS             complement(6103..7179)
                     /locus_tag="PKI35_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNX44574.1"
                     /translation="MKYEGKKLVWRDYQKEIAEDHYFYARSCIRQTFFPGSEMTFLRI
                     LKDELGKDVCDGAHHTSCTGIGYHSDIVPLETTMTVIARQFALMTEAGYENYAASCIT
                     SFGLYTEIIDMWHHFPETEDRAREYLFKATGREFKVPKNLAHASDIIYKFRYEIAAKA
                     KYRLVDKETGKPLKVVEHIGCHYSKMFPHKGVGGAEYPYVLTGMIEAWGGEVIDYPER
                     RHCCGFGFRQYIVQANRGFSIACSKKKFDSMEPYEPDMIITNCPGCPMFLDRWQYALS
                     EMEGKTYGKNGKGIPVFTYEEVAGIVMGYDPWEIGLQVHQVPAEPVLDKIGVEYDPDK
                     KFKGPNGEDLGRPVAPSYLKFYKE"
     gene            complement(7176..7886)
                     /locus_tag="PKI35_09085"
     CDS             complement(7176..7886)
                     /locus_tag="PKI35_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNX44575.1"
                     /translation="MGKLYDKLSQDVRFVESLKACINCGTCTAICPAAEFYNYEPRVI
                     ATTVQSQDDAKIEELLKSETIWYCGECMSCKTRCPRGNAPGLLIIALRSLSQDLGYFT
                     ESEKGRQQLALKRTIGEWILKYGYCVNPDIMNPRLHPEQGPIWEWEKEHIEPVMEKMG
                     ANYHGEGPGALRKISDADLQELKNIFDITGGTERFRKIEESSEKKADEMGLDCGHDID
                     SEYFDYVYTTNNSKHTRS"
     gene            8151..8840
                     /locus_tag="PKI35_09090"
     CDS             8151..8840
                     /locus_tag="PKI35_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX44576.1"
                     /translation="MPFTSGAQGYNWLKRVSLQFISEKEFDRIEKTSVQLSFKKGETI
                     LKQGGLSTHIVYLEKGIVKFNYENETNKNLILTIVSSPKILGGANLFYKDNNLFSIAA
                     VEDCEVVLIDANVLLSVMTDNAKFSIMLFQLASEMFKKSVINFVSLAHKQKEGRIADI
                     LIYLAVEVYHSNSFDLSLKRKELAEFAGCSTENVIMTLSRWQAENIIRIEGKHLDILE
                     MDRIKHISRIG"
     gene            complement(8891..11836)
                     /locus_tag="PKI35_09095"
     CDS             complement(8891..11836)
                     /locus_tag="PKI35_09095"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNX44577.1"
                     /translation="MKKYLLLLIVFWASFSIAQAQDEPVHPSIIGVGEYHGLSQPLRD
                     AAVLTDEEWERMEFDAMKPRNEELRERSYPFAAVALPKGNDPVWQKEYGRTQPPRAPI
                     VSFSGQTSPYYPSDANGAVGPNHFMQTINCVYAIYNKSGTLVAGPSNMNTLFSGVTGA
                     TYNDGDPVVLYDEMADRWVACEFSISGSNDYMLMAVSQTNDPTGSWHRYSFDVAEMPD
                     YEKMAVWLDGYYMGMNNSSGKDVYVFQRSQMLTGGTAQFIGFDNPNRPTTIDGFMCVP
                     PVDNDGASAPAGTPGMFITINDDAIGGGSDQLWIYKMTVNWVTPSSSTFARTQQINVP
                     AFDSNFGNNWDNIKQPGTSRELDAIPQVIMNAPQYRNFGTYQTLLCCHTVDVDATDHA
                     GIRWYELRTTNGTWSLRQSGTYAPDAHSRWMASIRLNGQGEIGLGYSISSTSVYPGIR
                     YTGQSAAAYAEGAGLLDLSEQTIVNGTTSQTAYNRWGDYSGMSVDPSDDKTFWFTTQY
                     GGSRQTKIASFQVAAAPATPDLIVQNPATTPTSIIQGATTTASCTVKNQGGADAGTSN
                     LKYYLSADVSYNSGDILLTTDAVAALTAGSSVSLSEVLTIPLETPVGTCYILFFADAD
                     AAVAEGNEANNVGYVQISVTSASGSPDLVVQSPATTPATVAAGSVTSATCTVYNQGTD
                     VAVASNLKYYLSVDNAYSSGDTYLATTAVASLAAGGSAAAGAALTIPSTTGAGTYYIV
                     FFADADAAVTESDETNNTGSVAITVTTITAGCTSSSQYPSSTLTPSKNWKTQNYIYAG
                     EYTAFNVTSGRTYYFSYCTTDGGSASYNSEMTLRNKTTDAFIAYSDDYCGDDAKIVWT
                     ATFTGTVKLVTTVSGCGTNSINTKLRYKYSTSKQTEDETGVETAEFQVYPNPTTGKIS
                     VESNNGFENVKKIIVYDASGRIVESVEISGRPDAVYSFDLGAKSPGYYLIKITGENVS
                     EQLKVVLTK"
     gene            complement(12042..12455)
                     /locus_tag="PKI35_09100"
     CDS             complement(12042..12455)
                     /locus_tag="PKI35_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNX44578.1"
                     /translation="MSKVIKSDTVTINRPAADIFSYITDFKNFGRMMPEQVENFKAET
                     DACSFEIKGMASLGLRITEKHPFTMVVMKAEGKLPFGFTFTCHTDAVSDNSTEVFLVI
                     DADMNPFMSMMAATPLQNFVNLLAGKLKEIMESAA"
     gene            complement(12452..13102)
                     /gene="pyrE"
                     /locus_tag="PKI35_09105"
     CDS             complement(12452..13102)
                     /gene="pyrE"
                     /locus_tag="PKI35_09105"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNX44579.1"
                     /translation="MRTNFEETALTTAEFLLQIKAIKLDNSNPFTWASGIKSPIYCDN
                     RKTLSYPKIRTYIRQEFVKCINEVYGTVDLIAGVATGAIAQGALVAQDLGLPFVYVRS
                     EKKSHGLENLIEGNVESGQSVVVVEDLVSTGKSSLNAVQALRDAGCNVKGMVAIFTYQ
                     LPDAEQNFKESGCPLQTLTNYDFLIKKALEQGYIKDTDLQSLIQWRENPKTWGLDK"
     gene            13101..13742
                     /locus_tag="PKI35_09110"
     CDS             13101..13742
                     /locus_tag="PKI35_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNX44580.1"
                     /translation="MAQRYRVFIDRKIIEFSDGVNDGVYANNQMITGFSSAQQLSADF
                     IKLLHSNEQSCLIVLTGERFDEAVKSFSSLFREIKAAGGIVRNAEGCFLFIKRHGLWD
                     LPKGKLEPDEKPESGALREVTEETGLANLSITAPPVSTFHIYPDQKGGFILKETYWYE
                     MLFKGNETPVPQLEEDITEVRWFARNELDEAVNNTYLSLKELLTDYFAGKASR"
     gene            13785..14243
                     /gene="coaD"
                     /locus_tag="PKI35_09115"
     CDS             13785..14243
                     /gene="coaD"
                     /locus_tag="PKI35_09115"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNX44581.1"
                     /translation="MKTAVFAGSFDPITRGHESIVRRALPLFDHIIVAIGVNQDKTGY
                     FPLEKRLEWIGQVFSDCPSVSVESYNGLTVDFCMRRNASYLIRGLRTSADFEFERSVA
                     QVNNRLTPSIETVFLLTQPEFVMITSSIVREVHKCGGNISQFIPETIQIG"
     gene            complement(14240..14794)
                     /locus_tag="PKI35_09120"
     CDS             complement(14240..14794)
                     /locus_tag="PKI35_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNX44582.1"
                     /translation="MRIIAGTNKGRILHPPKGLPIRPTTDLGKESLFNILNNRIDFEN
                     LTVLDLFAGTGNISLEFASRGAAEVVAVDINSKCIDYIRKASHELSLDKVFTVKTNAF
                     SFLKSVSRKFDVIFADPPYDMKEASEIPGLVFGNSLLAPDGWLILEHDVFKNFKDMPH
                     FAEVRRYGKVHFSFFSAEKEGQVD"
     gene            complement(14782..15702)
                     /locus_tag="PKI35_09125"
     CDS             complement(14782..15702)
                     /locus_tag="PKI35_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HNX44583.1"
                     /translation="MHLPTETGNNISSAGINCVLRLVDPFFGQADTLQYITSILFTGD
                     GCFISVHDKETRTFHVLLRYQFGLAPGEVFSPSGYESYRKAAMATIKAADEMGISMRN
                     PRVAFASTRNTLVPGSFYNADTAARCFELNYTAIQGEALNHEYIRALDAYNIYSIPPV
                     VQDVVSGNFPGVALHSVNGVVINSLLIDHAGSGDTIMLADIAERNLKIMVSGHESLIY
                     CNDFEVNTIEDVVYYIVFVSGQINPAGKIPLWLSGGINEGSRTHELLISYLENAAFIE
                     KPQGVSLAPVFRGISWHNFFTVFTVALCES"
     gene            complement(15674..16270)
                     /locus_tag="PKI35_09130"
     CDS             complement(15674..16270)
                     /locus_tag="PKI35_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44584.1"
                     /translation="MKSPILKVVLSLVILVLAYLIFNSIVTPVKFKNDLGSRGSVVID
                     KLKDIRTAEQLFKQINRHYTGDFDSLVQFIRTGKIPVVKMIPDPKDTTFTRTISDTLG
                     YVSIYDSIYSKKAYKLEELNVVPYSDGEKFELLAGQIDKGGVKVAVFEASTPIEFYSK
                     GLNKQLVINQKKEMENKNKFPGLKVGSMIDASTDGNWE"
     gene            16349..>16537
                     /locus_tag="PKI35_09135"
     CDS             16349..>16537
                     /locus_tag="PKI35_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44585.1"
                     /translation="MNFANVEQFSEYYPHKFLKKGTNLPGFAAIRNIPVASVVALRLR
                     VPSGAEKAADAAILTSKFI"
BASE COUNT         4353 a         3983 c         3713 g         4488 t
ORIGIN      
        1 acaatggtaa ccttcaccag cggattggca tgctccacaa gccagcttat tgctgcatgt
       61 cccgattcat ggtatgcaat cactttcttt tcgtgcgggg atataatctt gttgcgcttt
      121 tcaaggccgc caacgatcct gtcgatggca tccagaaaat cctgcttgtc gatttgtttt
      181 ttatcattac gggctgctat cagggctgat tcattgcaca cattggcaat atcggcgccg
      241 gagaagccag gcgtctgccg ggcaagaaaa tctacattaa cagtttcgtc cagtttcagg
      301 ttgcgcatgt gtaccttaaa aatagctttc cgttcttcaa ggtcgggcag ttcaacatgt
      361 atctgcctgt cgaaacgtcc ggcgcgcagc aaagccctgt cgaggatatc ggcgcggttg
      421 gtagccgcca ggatgataac ccccgcattg gtctgaaagc catccatttc ggtaagcaac
      481 tgattcagtg tattttcacg ttcatcgttc gatccggtca tcggattacg gccacgtgca
      541 cgtccgatag cgtcaatttc atcaataaaa atgatgcatg gcgctttatc ttttgcctgt
      601 ttgaacaggt cgcgcacacg tgaagcgccg acgccgacaa acatttcaac gaaatcggac
      661 ccggaaagtg agaaaaaagg cactttggct tcaccggcaa cagccttggc cagcaaggtc
      721 ttcccggtac ccggagggcc gaccaataaa gctcctttgg gtattttacc gcccagttcc
      781 gtatattttt tgggattttt taaaaattca acaatctcca tgatttccac cttggcttct
      841 tcaaggccgg caacatcatt aaaattggtg gtaaccatgg tatcgcggtc aaaaagctgg
      901 gccttcgatt tgccgatgtt gaatatctgg ccgccgccac cgccgccgcc tttgctcatc
      961 atgcgcataa tcaggaacca tccaccgatc aggattacga aaggtaatat ccatcccaac
     1021 acatccatcc cccagttctt gcgtgtaaca acctgtatgt aaacaggcgt tggattatcc
     1081 ttctgcgctt cctgtatctc tttttcaaaa gattcggccg aaactatctg gtagatataa
     1141 tggggtgtgg atgaagtaag ggaattccct tgtttcaggt ccttgtattt ttcaagcgac
     1201 agcctgtcct ttttaatgaa aatctcagcc tgctccttgt tgacgagtat aatcttttca
     1261 acatccttgc tgaccaacat tcccttaagc tccccccaac caatggtttt tgggcttgtt
     1321 cccatatttg aaaaatacag gaaggtgaaa atgacggcca gtatcccgta aatccaataa
     1381 aaattgaact taaacctggg attctttagt ttcggaagat taccgttaaa gggtttgcga
     1441 ttattgtcgc tatttggagt ttcttctgcc attattctgt tatataatcc tgattaacta
     1501 cttaggaaac atttgttcgc ccggcatcag cccagagacg ttccaactgg tagaagctgc
     1561 gcgtttcagc ctggaaaatg tggactacta catcaaaata atcgagtaat atccattcac
     1621 aattctcaaa accttcccgg tgccatggac tcagccctac tgcctttttt acttcagcct
     1681 caacggaatc agcaatcgcc tccacctgtg tacgtgatgt gccatgacaa attacaaagt
     1741 aatcggtaat agagttttgt aattggccaa ggttaagttt cacaatttcc tgcgctttac
     1801 gttccgccat accatgaact acaatgtcgg ccagcatatc ggacgcttca ctctttgcat
     1861 gttttgccat ctataattac ataattttca caaaggtaca tatttaattg ctttgtgcct
     1921 ttcggtatta cggccccttt ttatcaaaca tcgcgccccg gagccagata cgacattttg
     1981 tcatttacgg agtctgtatt taaacaacac ttttcaaaat ttgttgtacc tttaaaaatc
     2041 aaaattcaat aaacccatgc ggattatcgg ttcatccatc atcagcctcg atgaatcagc
     2101 atcaaccaac gattatgcct tagaattgct aaagtcatcg aaacctgccg acgggactgt
     2161 cgtcacaaca aaaaagcagt cggcagggcg gggacaggaa aacaattcct gggaaagtga
     2221 accgggtaaa aatctcaccc tgagcatcat cctatatccc catttcctgc ctcccgacag
     2281 gcaaagtctg ctttcgatgg ccatagccct gggcgtttat gatctcatta attcccatat
     2341 tacggcccag ccggtccgga taaaatggcc taacgatatt tatataggcg atcgtaaagc
     2401 ctgcggtatg ctcatccaga attcgattat ggggaaccgc tttgattacg ccattgccgg
     2461 tatcggactg aatgtaaacc aggaaaaatt catcagcgat gccccgaatc cggtttcgct
     2521 gaaaatgata gccggcaggg aattcccgac cgaagaatta ttatggcaac tttgcggctg
     2581 gctcgatacc cggtatcacc aactccggct gggcgacagg aaaggcataa gcgaagagta
     2641 cctcagcctg atgtacaggt ataatgagtg gcatgttttc gaaatacgac acagagctgc
     2701 tgaagccagg atcaccggga taaaccgtta cgggcaattg cagctcgaag aaagaaacgg
     2761 gacaagatca gaatgcgata tgaaggaagt aaaattcctt atctgatcta tcgtaattcc
     2821 ggattgcata ccggaaattt cacttctttt ccggcttcat gtaacaggct tgcctgttgc
     2881 cgggattgtg ttacgggtgc ttcaaacaag gttgaatccg agctttccgg catcctttca
     2941 ctaacaattt ccgccaggtc cataaccatt acgtctgaac cattgtcaaa ggtcttttta
     3001 cataaagggc aggctgtaac caacagatcc ggattcggct ccaataaaat tttcagcgct
     3061 tcgtccctga taacggtacg ttcattatag ttcattgcaa ggtcgccgac cgatcctccg
     3121 caacaaaggg catgttcacg ctgctgactg atatccgcca agcagaccat tttattaagg
     3181 agcctgcgcg gttcttcaaa aatgcccgag ccccgtccca attcacaagg atcatggtaa
     3241 acggccagaa ccggcgaagc atcaacctta atttttccgc tttccaccag cctgagcagg
     3301 tattgcgaat gatgaagcac ttcgatgcca ggcagtttat attcttcgct gaacaccttg
     3361 aaacaaatag gacaggatgt cacaaatgtt ttggctccag actttacgat cgtttcagta
     3421 ttttttcgga ttaattcacc ggctgcttcg ggtaatcctg ccatcatcag aggccttccg
     3481 caacataccg aaccatcctc atccatgaaa ctgaaatttt cgcctgaggc atccagaatt
     3541 ttcaccattg ctttcttgac cgaaggcgtc agatggccca tgcatcctgc aaaataaagc
     3601 atatcaaccc ggggttccgg tatctggatg ctgtcgaggt agttgtatga cggtgatacc
     3661 agcacgttat caatatatct ggaagccatc ctgattccaa cggtatcaat ctgaaccggg
     3721 caaacctggt gacacctgcc gcataacatg caattggcat cctgtatggc attctgttta
     3781 ccatagcgca aagagcgaag aaaataagcc ggggtagcat ccgttttccc ggcatagttc
     3841 atctggcaaa catcaatgca cacaccgcaa cgcggacagg agtgtatttc gatatcggta
     3901 aaactgtccg gttttttacc ggtttttatt ccgaaatgcc gcagataaat cagcagcact
     3961 tcggtgggta tatgcatgta ccgtgaccag ggaagcgtta caaaaaacac acccagcgcg
     4021 atggagtaag cccaccaggc aggataatac aggtgttcaa caggcaagaa cgaagcaaaa
     4081 gccattccca gggtattggt aaggaagcct cctccctggt aaacgccggc agtaaaactt
     4141 tcggccagca aacgcatcgg gaaaatcagc cagagagagg ttatggccca tttatcgagc
     4201 ttattgtgtt gggtggttct tttcatgccg aaccatttcg aacgggttct cttaatgaaa
     4261 gccagcacca atcccgaaag gataaacagc agcaggaaat ccatgatgaa ccggaaaaaa
     4321 cctggcagcg tatacaactc gaacggcaga gcgcgcttat catgaacaaa gaatttcaga
     4381 aatatgggat aatagggcgg gttgaggtga tttccgctat aaatcctcga ttcaaggttg
     4441 cccattacaa taagtaaaaa ccagccgaaa gcaaagctca tgtgcatgaa tcccagcagt
     4501 ttattctgac ggaacatacg cctgtgcaga aggctttcca taaacacttc ccaggttgcc
     4561 gggaagatct gcccgttcaa taacccatgg cctagttttt tgcgcccgct tctgtcgagt
     4621 gtagcaagcc aggcggtaaa acggatgatg atgactgcca gtaaaaacag catcccgcca
     4681 aaaaacggta taacaaatag gttaaaggtc ataaaacagt atttttcaat gccttatgaa
     4741 tctgtaagat caggtttcgt gtatttaccc cacggggaca aaccaattga cattttccgc
     4801 acaacatgca cttggtaact tcattttcca atccggaaac ctctccgcgg cgcaccagca
     4861 gaataagacg gcgtaaacta aaatcagtaa actgagcagc cgtgcaggta gcagcgcagg
     4921 taccgcaaga gatgcaaaga tttagcgacg gttcggcaat aataactgtg gaggccaatt
     4981 ttttgctgtt ggcatcataa tctatttgcc ggtctttgtt aaccgtaaac ccaaatctca
     5041 ctttttcttt tatgtcgctc atttcctaag ttttgccgga ttaccgatgt taactcttca
     5101 gatactttgc aaccgcaagc gccgccgaac gggcatcagc cagggtatcg gcaatgtttt
     5161 tcgggccggt acaggtcccg gcataaaata agccgggaat ttctgagata ttggcagaga
     5221 ggtgggcatc cgaaggaatg agaaaattat cattgtcttt cgccaggtgc agcatatcgc
     5281 gcaattgacg tgtcccggaa ggagattcca ttcccgccat cagcacaagc atatccaccc
     5341 ctattttcaa tggtttcccc aggagagtat cctcaacctt caggattact ttaccgttat
     5401 ggtcttcggt tgattcgctc aaacggccac ggataaactg gacattgtat ttttcctgtg
     5461 cttccttgta gagttcttcg taatgacgcc cgaacatgcg caaatccata tagaaacaaa
     5521 acacttcggt tcccgggagg aattccttga tttcgatagc ctgcttcact gcggttacac
     5581 aacaaacctt ggagcagtaa atattaccgg ctttttcatc gcgcgaaccc acacaatgca
     5641 caaatccgat tcttttcggt atttcaccgt ttttcatggt gactttgcca gctgagaaag
     5701 ctgcttcgag atcggcagaa gtgattacat tatcataaat ccggtatccg tattcttcct
     5761 ttttccgggc atcgaacagg gaaaagcctg tcgccatcaa cactgcatca gcttcagttt
     5821 gccaatcgtt accgctctca acgaaaaatg ttttcccgtt cggtttaatg gctgaaaccg
     5881 gcttaccggt gacaaggttt acaccgggat ccaggttctt cctcagcgaa tcaagcactt
     5941 cagcccccgg cctcctgtta ggaaacaaag cgtgccaatc tttaacatgc ccgcctgtag
     6001 aatcttcctt ttcgacgagt gttacccgga agcccatgga tgctaaggct gacgaggctt
     6061 caaggccggc ggcaccggct ccaattacag ctacatgcct gctcattctt tataaaattt
     6121 tagatatgac ggggcaaccg gccggccaag gtcttcgccg ttcgggcctt tgaatttttt
     6181 atcgggatcg tattcaacgc caatcttatc aagcaccggt tcagccggaa cctggtgcac
     6241 ctgcaatccg atttcccagg gatcgtaacc catcacaata ccagctacct cctcgtatgt
     6301 aaagacgggt atcccctttc cgttcttacc gtatgttttg ccttccattt cgctgagggc
     6361 atattgccag cggtcaagga acatcgggca gcccgggcag ttggtaatga tcatatccgg
     6421 ttcgtatggc tccatggaat cgaatttctt ttttgagcac gcgatagaaa acccccggtt
     6481 agcctgcaca atatattgcc tgaaaccaaa accacagcaa tgccggcgct cggggtaatc
     6541 gatcacttca cctccccagg cttcaatcat tccggtaagc acataaggat attcggcgcc
     6601 tcctacccct ttatggggga acattttgga ataatggcaa ccgatatgtt ccactacttt
     6661 aaggggtttc ccggtttcct tatccaccag gcgatatttg gctttagcag ctatttcgta
     6721 tcggaattta taaataatat cgctggcgtg ggcaaggttt ttgggcacct taaactcgcg
     6781 gccggtggct ttgaaaagat attcacgggc cctgtcctct gtttccggga aatgatgcca
     6841 catatcgatg atttccgtat aaagcccgaa actcgtaatg cacgaagctg catagttttc
     6901 ataaccggct tccgtcatca gggcaaactg tctggctatt acggtcatgg ttgtttcgag
     6961 cggcacgata tcggaatgat aaccgatgcc ggtacatgag gtatggtgcg ctccgtcgca
     7021 tacatctttt cccagttcat ctttcagaat gcgcaggaac gtcatttcgg accccgggaa
     7081 aaaggtctgc ctgatacaac tacgggcata gaagtaatga tcttcggcaa tctccttctg
     7141 gtaatcccgc cataccagtt tttttccttc gtatttcatg atctggtatg tttcgaattg
     7201 ttggtagtat aaacgtagtc gaaatactca ctgtcgatgt catgaccgca atccagcccc
     7261 atttcatcgg cttttttttc cgaactttct tcaatttttc tgaaacgttc cgtgccgcct
     7321 gtaatatcga agatgttctt caattcctgc aggtcggcgt ccgaaatttt cctgagagcg
     7381 ccgggacctt ctccatggta attggcgccc atcttttcca tgaccggttc tatatgctcc
     7441 ttttcccact cccagatcgg gccctgttcg ggatgaagcc tcggattcat aatatcgggg
     7501 ttaacgcagt atccgtactt gagtatccat tcgccaatag tgcgtttaag cgccagttgc
     7561 tgcctgccct tttccgattc ggtgaagtag ccaagatcct gcgacagcga tcttaaggca
     7621 atgatcagca atccgggggc attgcctctc gggcagcgtg ttttgcatga catacattcg
     7681 ccgcagtacc agatggtttc acttttgagc aattcctcaa tttttgcatc gtcctgcgat
     7741 tgcaccgttg ttgctattac ccgtggctca taattataga attccgctgc aggacaaatg
     7801 gcagtgcagg taccgcaatt tatacaggct ttcaggcttt caacaaacct gacatcctgt
     7861 gatagtttat cataaagctt gcccataact gctcctgttt cttactgccg gcatcaacat
     7921 tcaattatcg gaacatttta cttctctccc ttcgaagata ggccttaaac ggatacctgg
     7981 cttataccgg cagataaatt tacaatcgtt agctaagtta agaattcaaa aataagcctc
     8041 aaatctgtcc gtataattta tctttgcctt agtgttttca tgcaaaaaca ttaaataatc
     8101 aatgtcagag tattagatgt tatatttgtg gtttgctaaa attgaccttt atgccattta
     8161 cttctggagc ccagggatat aactggttaa aaagagtttc gcttcaattc atttccgaaa
     8221 aggaatttga caggatcgaa aaaacgtctg tccaacttag ttttaagaag ggcgaaacca
     8281 tcctgaaaca aggaggcctt tctactcata ttgtttacct ggaaaaaggg attgtaaaat
     8341 tcaattatga gaatgaaacg aataaaaatc ttattctcac cattgtttct tcgccaaaga
     8401 tattgggagg cgccaatctt ttttacaagg ataacaacct tttttcgatt gcagccgtag
     8461 aagattgcga ggtagtgctc atagatgcga atgttctgct gagtgtgatg accgacaatg
     8521 ctaaattttc aattatgctg ttccaactgg cttctgagat gttcaagaag tcggtgatca
     8581 attttgtcag cctggcgcat aaacaaaaag aaggaagaat cgccgatata ttaatctatc
     8641 tggccgttga agtttatcac agcaacagtt tcgatttatc gctcaaacga aaggaactgg
     8701 ctgaatttgc cggatgctcc accgaaaatg tgatcatgac attgtcgcga tggcaggctg
     8761 aaaatatcat ccggattgaa ggcaagcacc ttgacattct cgaaatggac cggataaaac
     8821 atatcagccg gatcggttaa aaaagagctg ctcctgaaca gaagcagctc tttcggcccg
     8881 aaggcaacca ttatttggtc agcactactt tcagttgctc gctgacattt tcgcctgtta
     8941 tctttatcag gtaataacca ggagattttg cacccagatc gaatgaatat actgcatccg
     9001 gccggccgga aatttctaca gattccacaa ttctcccgct tgcatcgtaa acgataatct
     9061 tcttcacatt ttcaaaaccg ttgttcgatt caaccgatat tttgccggta gtaggattgg
     9121 ggtatacctg aaattccgcg gtttctactc ctgtttcgtc ctctgtttgt ttggatgtgg
     9181 aatatttata cctgagtttt gtgttaatag agtttgttcc gcatcccgaa accgttgtga
     9241 ccagtttgac cgttcctgta aaggtggcgg tccagacgat tttggcatca tcgccgcaat
     9301 aatcgtcgga ataggcaatg aacgcatcgg tagtcttatt gcgtaaggtc atttccgaat
     9361 tataggaagc gcttcctccg tcggtcgtac aataagagaa gtaataagta cggcccgacg
     9421 ttacattgaa tgcagtgtat tcccctgcat aaatgtagtt ctgggtcttc cagttttttg
     9481 aaggtgtaag cgttgaactg ggatactggc ttgaacttgt acatccggca gtaattgttg
     9541 ttacggtaat ggccacgctg ccggtattgt tggtttcgtc gctttcggta actgctgcat
     9601 cggcatcggc aaaaaacaca atgtaatagg tccctgcgcc tgtggttgaa ggaattgtga
     9661 gcgcagcacc ggccgcagcc gagcctcccg ctgccagcga cgcaacggcg gtagttgcca
     9721 gataggtgtc gccggaacta tatgcattat cgaccgacag gtagtactta aggttagagg
     9781 ccactgccac atcggttccc tggttatata ccgtacaggt tgccgaagtc acacttccgg
     9841 ctgcaacggt ggcaggcgtt gttgcaggac tctgaacaac cagatcgggt gatcctgaag
     9901 cagaagtaac actgatctga acgtatccca cattattggc ttcattaccc tcggcaacag
     9961 cggcatcggc atcggcaaag aacagtatat aacaggtacc taccggcgtt tccaaaggta
    10021 ttgtcaggac ttcgcttaag gaaaccgagc ttccggccgt gagcgcagca acggcatcgg
    10081 tcgtcaggag aatatcgcct gagttgtaag atacatcagc cgaaagataa tacttaaggt
    10141 ttgaagtccc ggcatcggcg cctccctggt ttttgacagt acaggaagcc gttgttgttg
    10201 ctccctgaat gatggaagtg ggcgtcgttg ccggattttg aacaatgagg tcgggagtgg
    10261 cgggagcggc agccacctgg aatgaagcga ttttggtctg gcgagaacct ccgtattgtg
    10321 ttgtaaacca gaaagtctta tcatccgaag ggtcaacact catgcccgaa tagtcgcccc
    10381 aacggttata tgctgtttgc gaagtggttc cgtttacgat tgtctgttca ctcaagtcca
    10441 gtaatccagc accttcagca taggcggcgg ctgactgacc ggtgtaccgg attccgggat
    10501 aaaccgaagt actggatata gaatatccca ggccaatttc accctggccg ttcaggcgga
    10561 tactggccat ccaccgtgag tgcgcatcgg gggcataggt acccgactgg cggagcgacc
    10621 aggttccgtt tgttgtacgc agttcatacc atcttatacc ggcatgatcg gtagcatcca
    10681 catctacggt atggcaacac agcaaggttt ggtaagtgcc gaaattccgg tactggggcg
    10741 cgttcatgat aacctgcggt atagcatcaa gttcacggga tgtaccgggt tgtttgatgt
    10801 tgtcccagtt attcccgaaa ttactgtcga atgccggaac atttatctgc tgggtacggg
    10861 caaaggtaga cgacgaagga gtgacccagt tgacggtcat tttataaatc cagagttgat
    10921 cggaaccgcc gcctatggca tcgtcgttaa tagtgatgaa cattcccgga gtaccggccg
    10981 gcgcggaggc tccatcgtta tctacaggcg gcacacacat aaaaccatct attgtggttg
    11041 gcctgtttgg attatcgaat ccgataaatt gtgctgtacc acctgtcagc atctgcgagc
    11101 gctggaaaac atatacgtct ttgcccgagg aattgttcat tcccatataa tagccatcca
    11161 gccagacagc cattttttca taatcaggca tttcggcgac atcaaaggag taacggtgcc
    11221 atgaacctgt cgggtcattg gtctgcgata cagccatcag catataatca tttgatccgc
    11281 tgattgagaa ttcgcacgca acccatcggt cagccatttc atcgtacagc acaaccggat
    11341 cgccatcatt gtaagttgcg cccgtcaccc cgctgaagag ggtattcatg tttgaagggc
    11401 ctgctaccag cgttcccgat ttattataaa tggcgtaaac gcagttgatg gtctgcatga
    11461 aatggttggg ccccacagcg ccattggcat ctgacggata gtagggcgat gtttgtccgc
    11521 tgaacgatac gattggcgct cttggcggct gggttctgcc atattccttc tgccaaaccg
    11581 ggtcattacc tttgggcaat gccacagcgg caaaaggata ggaacgctcc cgtaactctt
    11641 cattccgggg tttcatggcg tcaaattcca ttctttccca ttcttcgtcg gtcagcacgg
    11701 ctgcatcccg caagggttgc gacagcccgt gatactcacc aaccccgata atggaaggat
    11761 gaacaggttc atcctgtgcc tgggcgattg agaatgatgc ccagaacacg atcaggagta
    11821 ataaatattt tttcatggtc ggacggtttg gttgataaat ggctggatta caaattctga
    11881 aatatatagt aaatataata ttaaatcagg atacttagaa cacttcttaa catatttcat
    11941 tacagctttt acaataattt tcagggatac agactttaaa aaacaacaat gcccggcatt
    12001 tataaccggg catcgcataa tttaaaacca gtttgctgtt atcaggcagc cgattccatt
    12061 atttctttca atttgccggc cagcaggtta acgaaattct gcaaaggggt ggcagccatc
    12121 atggacatga aaggattcat gtcggcatcg atgaccagaa acacttctgt tgaattatca
    12181 gaaaccgcat ctgtatgaca ggtaaaagta aatccgaaag gcagcttacc ctcggctttc
    12241 atgacgacca tggtaaatgg atgtttttcg gtaattctca aacccagaga tgccattccc
    12301 ttgatttcga aggaacaggc gtcggtttcg gccttaaaat tttctacctg ctcaggcatc
    12361 atccggccaa aatttttaaa gtcggtgata tagctaaaaa tatcagccgc cggacggttt
    12421 atggttaccg tatcgctttt tatgaccttg ctcatttatc gagtccccag gttttgggat
    12481 tctcccgcca ctgtatcagc gactgcaggt cggtatcttt aatataaccc tgttcaaggg
    12541 cttttttaat caggaaatcg taattggtga gtgtttgaag agggcaaccg gattctttga
    12601 aattctgttc agcatcggga agctgatatg taaaaatcgc caccattcct ttcacgttgc
    12661 atcctgcatc gcgcaatgcc tgcacggcat tcaggctgct cttgccggtc gaaacaaggt
    12721 cttcaaccac cacgaccgac tgtccgctct ccacattgcc ttcaatcagg ttttcaagtc
    12781 cgtggctttt cttctccgaa cgaacataca cgaatggaag gcctaggtcc tgcgctacca
    12841 gcgctccctg ggcgatggca cccgtggcta ccccggcgat caggtcgact gtgccgtaca
    12901 cttcattgat gcattttacg aactcctggc ggatataagt cctgattttc ggataggaca
    12961 aagttttgcg gttatcgcaa taaatcggcg attttattcc cgatgcccat gtaaacggat
    13021 tactgttgtc gagttttatt gctttaattt gcaacaggaa ttcagccgta gtcagggcag
    13081 tttcttcaaa atttgttctc atggcgcaaa gatacagagt ttttatcgat cgtaaaataa
    13141 ttgagttttc agacggtgtg aacgatggcg tttatgccaa taatcagatg attaccggtt
    13201 tttcgtccgc acaacagtta tcggctgatt tcataaagtt actacatagt aacgagcaat
    13261 catgcctgat tgttttaaca ggcgaaagat ttgatgaagc ggtaaaatcg ttcagctccc
    13321 tgtttcgcga aatcaaggca gccgggggaa ttgtccggaa cgcggaaggc tgtttcctgt
    13381 tcataaagcg gcatggcctg tgggacctgc ccaagggaaa acttgaacct gacgaaaaac
    13441 cggaatccgg agccttgcgc gaagtgaccg aagaaaccgg tttggcaaac ctttccatta
    13501 cggctcctcc ggtttcaaca tttcacattt acccggatca gaaaggcgga tttattctta
    13561 aggaaacata ctggtatgag atgttattca aaggaaatga aacgcctgtg ccacaactgg
    13621 aagaagatat taccgaagtc agatggtttg cccgcaacga attggacgaa gctgtcaata
    13681 acacctacct gtcgctcaaa gagttgttga cggattattt tgccggaaaa gcctcccggt
    13741 aggccactga tattttctaa gtaattttgt aaaaaataaa ccctatgaaa acagccgttt
    13801 ttgccggttc gttcgacccg attacccgtg gacacgaatc cattgtccgg agggctttgc
    13861 cccttttcga ccatatcatc gtagctatcg gcgtcaacca ggataaaacc ggctatttcc
    13921 ccctcgaaaa acggcttgaa tggatcggac aagtcttcag cgactgtcct tcggtaagcg
    13981 tggaaagtta taacggactc accgttgatt tctgcatgag aaggaatgct tcctacctta
    14041 tcaggggatt gcgcacttcc gccgattttg aatttgagcg ttccgtagca caggtcaaca
    14101 accggcttac tccctccatc gaaaccgttt tcctgcttac gcaacctgag tttgtgatga
    14161 taacctcctc aattgtccgc gaggtgcata aatgtggcgg taatatcagc cagtttattc
    14221 ctgaaaccat tcagataggc taatcaacct gcccttcctt ttcagcggag aaaaaggaaa
    14281 aatgaacttt accgtaccgc cttacctctg caaaatgcgg catatcttta aagttcttga
    14341 atacatcgtg ttcaagaatc agccagccat caggggcaag gagtgagttg ccgaaaacca
    14401 gaccgggaat ttcagaggct tccttcatat catagggagg atcggcgaag atcacatcaa
    14461 acttccggga cacggatttc aggaagctga aggcattggt ttttaccgta aaaaccttat
    14521 cgagcgacag ttcatgcgaa gccttgcgta tataatcaat acattttgaa ttgatatcca
    14581 ccgctaccac ttctgcggct ccgcgtgaag caaattcgag ggagatattc cctgtaccgg
    14641 caaaaagatc cagcaccgta agattttcaa aatcaatacg gttgttaaga atattaaaaa
    14701 ggctttcctt gcccaggtcg gtggtagggc gtatcggcag ccctttggga ggatgcagta
    14761 tcctgccttt gtttgttcct gctatgattc gcacagtgca acagtaaaaa ccgtgaagaa
    14821 attatgccat gaaattcccc ggaataccgg cgccagggaa acgccctgag gcttttcgat
    14881 aaaagctgcg ttttcaaggt agctgatcag cagttcgtgg gtgcgcgatc cttcatttat
    14941 cccgcctgac agccaaagag gtatcttccc ggccgggttt atctgtccgg aaacaaaaac
    15001 gatataataa acgacatcct caatagtatt gacctcaaaa tcattgcaat aaataaggct
    15061 ttcgtgaccg ctcaccatta ttttcaggtt tctttctgct atatcggcca acatgattgt
    15121 atccccggaa ccggcatgat ctatcagcaa tgaattgatt acaacgccat tgacgctatg
    15181 aagggcaacg ccggggaaat tccctgaaac gacatcctga acaaccggtg ggatactgta
    15241 aatattgtat gcatccaggg cgcggatata ctcatggtta agcgcttcgc cctgaattgc
    15301 cgtataattt aattcaaaac agcgcgccgc ggtatctgca ttgtaaaaag atccaggcac
    15361 cagggtatta cgggttgaag caaaagcaac tctgggattc ctcatggata ttcccatctc
    15421 atcggcggct tttatcgttg ccatggccgc ttttcggtaa ctctcatacc cggatggact
    15481 aaaaacctca ccaggcgcca gcccgaactg gtaccggagc aatacatgaa aggtacgcgt
    15541 ttccttatcg tgaaccgaaa taaaacatcc atccccggta aagagaatgg atgtgatata
    15601 ttgcagcgta tcagcctgac cgaaaaacgg gtcaaccagc cgaaggacgc aattaatgcc
    15661 ggctgacgaa atattattcc cagtttccgt cggtagatgc atcgatcatt gagccaactt
    15721 tcagaccggg gaatttattt ttattctcca tctccttctt ctggttgata acaagctgtt
    15781 tgttcaaccc tttggaatag aattcaatgg gagtcgaagc ttcgaaaacg gcaactttta
    15841 cgccaccctt gtcgatctgc ccggccagta attcgaattt ttcgccgtcg gagtaaggta
    15901 ccacgttcag ttcctccagc ttataggcct ttttcgaata tattgagtcg taaatgctta
    15961 cataaccaag ggtatcggaa atggtgcggg tgaaggtagt atccttagga tcgggaatca
    16021 ttttcaccac aggaattttg cccgtcctga taaattgtac aagtgaatca aaatcacctg
    16081 tataatgcct gttaatttgt ttgaaaagtt gctcagccgt acggatgtct ttcaatttgt
    16141 ctattacgac agatccccgg gaaccaaggt cattcttaaa tttcacaggt gtgacgatgc
    16201 tgttgaaaat caggtaggcc agtacaagaa ttaccaggct taaaacaact ttaaggatag
    16261 gactcttcat gttttaatga ttttgtaaaa gtttcggagt atttacttcc agtctctaat
    16321 tcgctacaat accggacttc cccggttttt gaattttgca aatgtagaac aattttcaga
    16381 atattatcca cacaaatttt tgaaaaaagg gacgaacctt cccggttttg ccgccatccg
    16441 taacatccct gttgccagcg tggtagcttt gcgtctacgg gttccatctg gtgcagaaaa
    16501 agcagcggat gcggcaatcc tcacttcaaa atttatt
//
LOCUS       DAOPYV010000068        14921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_4340_length_14921_cov_13.413965, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000068 DAOPYV010000000
VERSION     DAOPYV010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14921
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4340_length_14921_cov_13.413965"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(616..1299)
                     /locus_tag="PKI35_09140"
     CDS             complement(616..1299)
                     /locus_tag="PKI35_09140"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNX44586.1"
                     /translation="MSEMKKYAIIVAGGTGSRLSSDTPKQLLLLDGKPLIMYSIETFY
                     RYDPGLHIIVAMHPAFMGKWTALCAGSGFTIPHQLVPGGETRFHSVKNALALVPGNGL
                     VAVHDAARPLVTVDLISRTFSEAAGNGTAIPCVPVNETVRSIEKGKITLVDRSRLRIT
                     QTPEVFDISLLQHAYQQPYREEFTDDGSVVEATGRQVCLVEGEATNLKVTLPGDLEIA
                     GVLLKRMKP"
     gene            complement(1876..3069)
                     /locus_tag="PKI35_09145"
     CDS             complement(1876..3069)
                     /locus_tag="PKI35_09145"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HNX44587.1"
                     /translation="METIRKIYKTGLGPSSSHTMGPRFAAEKFRAKNEAATHIHITLY
                     GSLAATGKGHLTDKVLRESFPEDKVKIIWEKDIVLPQHPNGMKFEAFDDKGNLLDEWT
                     VFSVGGGDIEDEETKLQKVTMYPHSNMNDILKWCQHNGRTLWEYVEIHEGKEIWDFLA
                     DVWKTMKESIDHGLEHEGVLPGILNLPRKASSYHIKAKSSTGSLQKRAMLFSYALAVS
                     EENAGGGTIVTAPTCGSCGVVPAVLFHIQNTYYYPDARILRALATAGLIGNLVKENAS
                     ISGAQVGCQGEVGTACSMAAGAAAQLFGGTPAQVEYAAEMGLEHHLGLTCDPVCGLVQ
                     IPCIERNAIAAGRAMDASAYAVLSDGRHRVSFDKTCLAMKETGHDLPDIYKETSEGGL
                     ALLIK"
     gene            complement(3184..4239)
                     /gene="queA"
                     /locus_tag="PKI35_09150"
     CDS             complement(3184..4239)
                     /gene="queA"
                     /locus_tag="PKI35_09150"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNX44588.1"
                     /translation="MKLSQFRYHLPSDLIAERPTESRDESRLMVIDRKTKSIEHKMFT
                     DVLDYYHDGDVFILNNTKVFPALLTGEKEKTGAKITVFLLRELNSEARLWDVLVDPAR
                     KIRIGNKLYFGDDDSLVAEVIDNTTSRGRTLRFLFDGPYDEFKRTIESLGKTPIPEEL
                     QRMRDIEPEDKERYQTIYAKNEGAVAAPSAGLHFSRELMKRLELQGVTFAEVTLHAGL
                     GNFRAIDVEDLTKYKMDSEEVIIEDETVRIVNKAKEEKKNVCVVGTTTMKAVESSVTI
                     SGMLKPFRGWSNKFIFPPYDFSIADSLITNLHLPQSPMMMMVAAYAGYDFLMKAYKEA
                     VQQKYRFFSYGDAMLIL"
     gene            4508..6253
                     /gene="aspS"
                     /locus_tag="PKI35_09155"
     CDS             4508..6253
                     /gene="aspS"
                     /locus_tag="PKI35_09155"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008777620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNX44589.1"
                     /translation="MLRTHTCGELNMAAAGQTVTLTGWIQRSRDLGGMTFIDLRDRYG
                     ITQLVFNMDTRAALCGEARKLGREFVISVTGLVAERSNKNPKLPTGDIEIIAEKFEIL
                     NESKIPPFTIEDNTDGGDELRMKYRYLDLRRPALKRNMELRHRMAFQARSYLDSLNFI
                     EIETPVLINSTPEGARDYVVPSRVNPGEFYALPQSPQTFKQLLMVAGYDRYFQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFITQEDILTIFEGLIKHLFKTVKNIDIVTLPRITWAD
                     AMKKYGSDKPDIRFGMEFTELTDLVKGKDFKVFDDAELVVGINAEGCAEYTRKQLDEL
                     TDFVKKPQIGAGGLIYLRYAADGTLKSSVDKFYSPDDLQKWAEAFGAKPGDLMLILAG
                     KAMKTRKALNELRLEIGGRLGFRNPEIFAPLWVYDFPLLEWDDETQRYYAMHHPFTSP
                     KPEDIPLLDTDPGAVRANAYDMVINGVEVGGGSIRIHTKDMQRKMLQLLNFSAEEAQA
                     KFGFLMNAFEYGAPPHGGIAFGFDRLCSVLGGSDSIRDYIAFPKNNAARDVMIDSPSR
                     ISEEQLKELSLKISI"
     gene            complement(6555..6845)
                     /locus_tag="PKI35_09160"
     CDS             complement(6555..6845)
                     /locus_tag="PKI35_09160"
                     /inference="COORDINATES: protein motif:HMM:NF040306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6040 family protein"
                     /protein_id="HNX44590.1"
                     /translation="MGTTTELLTIICLCAVIIIIEIIMAFWKPDYMEKYRKSPIPLAG
                     LLLLLALFGCRLFSGYAIQKVAPVTGIIYLILVAYFVSGVYKYLKSRKRREN"
     gene            complement(6985..7368)
                     /locus_tag="PKI35_09165"
     CDS             complement(6985..7368)
                     /locus_tag="PKI35_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44591.1"
                     /translation="MNYTRKDHELPLATSEWGWKFHHIGIPVNEPFPGEEHIAHLGIY
                     AGGFKNSPYGIEFMRFEPHCKVHELVKKIPHVAFVVDDMETALIDKELLGEVNSPSPG
                     LKVAMILHNGAPVELMEFSSDREAG"
     gene            complement(7827..9560)
                     /locus_tag="PKI35_09170"
     CDS             complement(7827..9560)
                     /locus_tag="PKI35_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HNX44592.1"
                     /translation="MNKRLLIRDVTLRDGQQSLFATRMSQQQVDRVLPLFKQAGFYAM
                     EVWGGAVPDSVMRYLNEDPWDRLEKIRDGIGNKSLLTALSRGRNLFGYNPYPDEVIEG
                     FCRNAIKSGVSIMRIFDALNDISNMKATIRFVKENGGVADCCIVYTVDPKYSKRERLR
                     ALLHAKMLPTNVFTKAYFVNKARELEQEGADMITIKDMAGLATPGKTGRLVRMLKESL
                     SIPLDFHTHCTPGYGVASTLMAIVNGADIVDTAIMNFAGGTAAPPFELIQLFCDRMGI
                     ETGVDRQAVLQLNLELREIRRELADFDATKAFPREFNFVEDKLPKDIDELVEMAIAHA
                     KADKEEELLEVCHRLEAWFGLPEANDIIREAEIPGGMYSNMLAQLKQLKLEKLLPRAM
                     ELIPLVRLASGCPPLVTPTSQIVGVQAVNCAIDESKGAPIYTTKSIQYVNLVKGSYGK
                     TPVAIDPDFRYKIAGVKEEIPYDSRYYKKQANPIYEEYGGVKMAQNEKEELLLELFPS
                     VANDFLKKRMEDVYLTEIHAVEAEKQRRIDEEKEKYESLSPEQKEKRLLDGLYHYPWS
                     SSEEDPEIGHS"
     gene            9850..11496
                     /locus_tag="PKI35_09175"
     CDS             9850..11496
                     /locus_tag="PKI35_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HNX44593.1"
                     /translation="MKKYYLMTAFVAIAVILNTGCKQPGNKQTNTQLSEMQQKVNEYA
                     VVRLTTDLSKLTDKEKQMIPILVEVSEIMDDIFWEQTLGNKAAFFDTIKDEATRQFAM
                     INYGPWDRLDENKPFIAGIGKKAPGANFYPADMTKEEFEKWNNKDKASQYTLIRRNAD
                     GSLKCVWYHEAYADKIKKASDLLLKAAALAEDPGLKKYFELRSKALLTDDYFASDMAW
                     MDMKNNTIDFVAGPIENYEDELFGYKTAQEAAILVKDKDWSKKLEKYAAMLPALQKEL
                     PCDPKYKKEVPGTSSDLNAYDILYYAGNSNCGGKTIAINLPNDEKVQLAKGSRRLQLK
                     NAMQAKFDKILVPIADVLIDPSQRANIKFDAFFGNVMFHEVAHGLGIKNTVNGKGNIR
                     EALKEQYSGWEEGKADILGLYMVTKLIEKGELKGVTVEDAFVTYMAGLIRSVRFGAAE
                     AHGKANMMCFNFFEDKGAFKLSADGTYKVDMEKTRKAMNEWSAFILKIEGEGDYQQAV
                     SYMAANGKVRDELKTGIEKLKSANIPKDVVFEQGVKVLGL"
     gene            complement(11829..12347)
                     /locus_tag="PKI35_09180"
     CDS             complement(11829..12347)
                     /locus_tag="PKI35_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44594.1"
                     /translation="MKRILKALIVAALVITNGIMCSCSREEKSHTLQTDESEFSNSNV
                     SQNENAAENYLTEPESFQYTVGSAKGKNGNHPKSINYKVYNTETVFVVELTYNLLPYE
                     AYETSSIRITVNGQVMHQDILNHNIAICTFSLSDLKPGWKTGDQIEWSFTETGNTNNP
                     ISYNDFFSLTEI"
     gene            12720..14876
                     /locus_tag="PKI35_09185"
     CDS             12720..14876
                     /locus_tag="PKI35_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNX44595.1"
                     /translation="MKKYLLALCMLVAIFAKAAETPDEGMWLPMFVERLNYVDMQKMG
                     LRLTPEELYSINHSSLKDAIVSLGGFCTAEVVSPEGLLFTNHHCGYGNIQKHSTVDHD
                     YLTDGFWAMSKAEELPNEGLTVSFLERMADVTTEVLGVVTSEMDEQARQKAISGKIDE
                     LKKAASEEGKYQVELKSFFNGNEYYMFVYQVYKDVRLVGAPPSSIGKFGGDTDNWMWP
                     RHTGDFSIFRIYTAPDGSPAEYSKDNVPLKPKHYLPVSLKGYEKNDFAMIWGFPGQTD
                     RYRTSWGVDATLNDINPVITKGLGKILEIQKAGMDADNSVRIAYADNYAGLANFWKNK
                     VGETRDLKRLGVVAKKQELEKQFADWVNATEARKQKYGNVLSTFSDVYAKYKDQNLYS
                     VLWHTQMILFGSQAFRAPGMAEGLETVLKSGVKGEELAKQLVKYREFSAEQFKEYNIG
                     IEQNVLAAVLEMFYTGVPKEQQPEILKTVESKYKGCFHAYAADLFKKSIWYTPENFNA
                     FLDKPSLKKLQNDPTVKLYSSFMEIFKKIQESNKELSNQLRVAQRLFAAGLREMEPDK
                     SFYPDANSTMRMTYGKVLDYYPADAVHYKYYTTLDGVMAKEDPTNEEFIVPARLKDLY
                     QKKDYGQYGVNGTMYTCFLTDNDITGGNSGSPVLNADGQLIGLAFDGNWEAMSGNILF
                     EPELQRTICVDVRYVLFIIDKFAGATNLINELTLVK"
BASE COUNT         4033 a         3476 c         3478 g         3934 t
ORIGIN      
        1 ttgtttcaac acgcaagatg tgtgtttgca gataacgaat taaagccttt ttgtttgaag
       61 aagtataggt aattacgttt attcacgggg cggctcccgg gccaaatgcg gtccagtcac
      121 cccgtgaatt tttgagttga taagcaccaa cttgcaaaat gtcgctaccg gaattattcg
      181 cagggcgact ccccggcact gagcgtcgcg gccgccctgc gaataaaaac acaaccgccc
      241 tttgattatt tcatattatc tgatattatt tcccttctga aattcagtat gttcaaaaga
      301 tttttatatc aggcaaacaa cttacgccca taaaacattg aatactactc attgtatttg
      361 ccggcttatc cttcgccttt aattaattga ccgggatggt ataatatagg cagaatattg
      421 acagggaaca gataccatat ttgtgccctt tggccacaga gggaaaagag tgaggtatta
      481 acagggtttc aagtcattaa cccgttctgc cggtttgtaa agaaagctag tttattataa
      541 ttgccagtct acttactttc cccataatag ccggccggtt aatttttcat gaccatgaac
      601 cggccattca aaaacttacg gcttcatccg tttcagcaat actccggcaa tctcaagatc
      661 tcccggcagg gtaactttca ggtttgtcgc ttccccttca accaggcata cctgccgtcc
      721 cgttgcttcc actaccgatc cgtcgtcggt aaattcttcc cggtacggtt gctgataggc
      781 atgctgaagc agtgaaatat caaaaacttc cggtgtttgg gtaatacgaa gccggcttcg
      841 gtcgaccagg gtgatttttc ctttttcaat acttctcacg gtttcattta caggaacaca
      901 gggaatcgcc gttccgtttc cggcagcttc cgaaaaagta cgtgatatca ggtcaacggt
      961 aaccaatggc cttgccgcat catgaacggc aaccagaccg ttaccgggaa caagcgccag
     1021 cgcgtttttc accgaatgaa aacgggtttc accgcccgga accagttgat ggggaatggt
     1081 gaatccgctt ccggcgcaaa gggctgtcca tttgcccata aaggcaggat gcatggcaac
     1141 gatgatatgc aaaccgggat cgtaccgata aaaagtttcg atggaataca tgatcaaagg
     1201 cttcccgtcg agtaacagta gctgtttggg tgtatcgctg cttaaccggc tgcctgttcc
     1261 gccggcaact atgatggcgt atttcttcat ttctgacatt tatgggctgt tgtgaagccg
     1321 ggtatcatga aataacaatg gcaatgatat tgaaaaatat cataaacccg ttgaatttta
     1381 taaatattct ttgggtttca ggggtaaggg aagtgaacca ccaagcacag aggatgcacg
     1441 gggattttta gaaagatttt ggtagggaga ggaggccgcc gcgtcaatca ttgcctccgg
     1501 caatgatctc ctcgcggtgt ttcaatatga aagaggacta aaaaatgacg gctctgcgag
     1561 ccccggtgtg ccggggcgaa gcagcctctt gcccttagca caaccacccc tgcagcctct
     1621 tgccttttaa taccgccgcc ccaagaacta cctcccctcc gttcgcgcac gtctcatgac
     1681 gcatacgctg agagctgccc ttatttacca agggcacgaa gggcactgag gccgccgtgg
     1741 cggacactaa gaaaatatgc aatgctctgt gctttgtgtt cttcgtggct ccgtggtaaa
     1801 taatcctaag tgcctctaag tgatctccgt gcctgagtgg taaaaaacta atcgccttcg
     1861 cggcctttta ctttcttatt tgatcagcag ggccagtccc ccttccgagg tttccttata
     1921 gatatccggc aaatcgtgtc ctgtttcctt catggcaagg catgtcttgt cgaagcttac
     1981 cctatggcgt ccgtcggata aaacggcata ggctgaagca tccattgctc tccccgctgc
     2041 aatggcatta cgttcgatgc agggaatctg aaccagtccg cagaccggat cgcaggtaag
     2101 cccgaggtgg tgttcgagcc ccatttcggc ggcatattcc acctgcgccg gcgttccgcc
     2161 gaacagttgc gctgctgctc ccgctgccat ggaacaagcc gtgcccactt cgccctgaca
     2221 tcccacctgg gcgcctgaaa tagaggcatt ctctttcacg aggttgccga tcagaccggc
     2281 agtggcgagc gcccgtaaaa tcctggcatc aggataataa taggtgttct gtatatggaa
     2341 aaggacggcc ggtacaaccc cgcaggaacc gcaggtaggc gccgtgacga tggtaccgcc
     2401 tccggcgttt tcttccgaga cggctaaagc gtatgaaaat aacattgccc tcttttgcag
     2461 ggaaccggtc gaacttttag cctttatatg gtatgatgat gcttttcggg gcagattgag
     2521 aattcccggc aatacgcctt catgctccag gccatggtca atggattctt tcatcgtttt
     2581 ccacacatcg gccaggaaat cccagatttc ttttccttca tgtatttcaa catattccca
     2641 aagagtgcgg ccattatgct ggcaccactt caagatatcg ttcatgttgg aatgcgggta
     2701 catggtaact ttctgcagtt tggtttcctc atcttcgata tcgcccccgc ccacgctgaa
     2761 aaccgtccat tcatccagca ggttgccttt atcatcaaag gcttcaaact tcataccgtt
     2821 gggatgctga ggcagaacaa tatctttttc ccagatgatt ttcaccttat cctcagggaa
     2881 cgattcgcgc aaaaccttat cggttagatg ccctttgccg gtagcggcca ggctgccgta
     2941 caaggtgata tggatatgag tggcagcttc attttttgcc ctgaattttt cggcggcaaa
     3001 ccggggcccc atggtatgac tactcgacgg cccaaggccg gtcttataaa tttttcggat
     3061 ggtttccata taccttttat tagtgaacaa atttacatca ccggttaccg tttatgtaaa
     3121 cacgggctgc caaaaaaaag cccctcggtt gaggagctaa acgatcgttt ttttaaggaa
     3181 tggttaaagt atcagcatgg catccccgta tgagaagaag cgatatttct gctgaacggc
     3241 ttccttgtat gctttcatca ggaaatcgta accggcataa gcggccacca tcatcatcat
     3301 gggtgactga ggcaggtgta ggttggtgat caggctgtcg gcaatgctga aatcataggg
     3361 ggggaatatg aatttgttgc tccagccgcg gaaaggtttc agcatacccg aaatggttac
     3421 cgacgactca acggctttca tggtggttgt accaaccacg cagacatttt tcttttcttc
     3481 cttggctttg tttacaattc tgacggtctc atcctcgatg atcacctctt ccgaatccat
     3541 cttgtatttg gtaaggtctt ccacgtcgat ggcgcggaaa ttgcccaggc cggcatgaag
     3601 cgtgacttcg gcaaaggtaa caccctgaag ttcgagacgt ttcatcagtt cgcggctgaa
     3661 atgcagccct gccgaaggag cggcgaccgc gccttcgttc ttcgcataaa tggtctgata
     3721 tctctccttg tcctcaggct caatatcacg catgcgctgt agttcttccg gaatgggcgt
     3781 ttttccaaga gattcaatgg tacgtttgaa ttcatcgtaa ggcccgtcga aaaggaaacg
     3841 cagggtacgg ccacgggatg tggtattatc aatgacctcg gcgaccagcg aatcgtcatc
     3901 gccgaaatat aatttgttgc ctatacggat ttttctggcc ggatcaacca atacatccca
     3961 aagccgggct tcactgttga gctcgcgaag gagaaaaacg gtgatcttag cgccggtttt
     4021 ttctttttct ccggtaagga gggcggggaa aaccttggta ttattgagga taaaaacatc
     4081 tccgtcatga taataatcaa gtacatcggt gaacatctta tgttcaatcg atttcgtttt
     4141 gcggtcaatc accattaaac gtgattcatc cctgctttca gtaggccttt cggcaataag
     4201 gtcgctcggt aaatgatacc ggaactgcga aagtttcatc cgtgtagagt atttttctca
     4261 aaaaaggctg caaaattaat gatttttttg ctgaaaatca agactttcgc taaactaaaa
     4321 acttccattt aactcttttt aactttattg tactcatggc atttgaaaat attgtagcat
     4381 attatcgaag ttgtataatt gcaaattctc cgtattacac tgataataaa caggggaaat
     4441 aggcaaatgc acatgggttc ccttccatcc tttttttcta actttgccgg cttaaaaaca
     4501 tggaattatg ctccgtacac atacctgtgg agaactcaac atggccgccg caggccagac
     4561 tgttaccctt accggctgga tacaacgctc gcgcgatctg ggcgggatga cttttatcga
     4621 cttacgtgac cgctatggca tcacgcaact ggttttcaat atggataccc gtgccgccct
     4681 ttgcggggaa gcccggaaac ttggccgtga atttgtgatt tcggttaccg gactggtagc
     4741 cgaacgcagc aacaagaatc cgaaactgcc taccggcgat attgagatta ttgctgagaa
     4801 atttgaaata ctgaacgagt cgaaaatacc tccgtttacg attgaagaca ataccgacgg
     4861 cggcgatgaa ctccgcatga agtatcgtta tcttgatttg cgccggcccg cgttgaagcg
     4921 taacatggaa ctgcgccacc gtatggcatt ccaggcccgc agttatctcg acagcctgaa
     4981 tttcatcgaa atagaaacgc cggtcctcat taatagcacc cccgagggcg cccgcgacta
     5041 cgtggtacca tcgcgcgtta accccggaga attttacgct ttgccccagt cgccgcaaac
     5101 tttcaagcaa ctgctgatgg tagccggtta cgaccgctat tttcagattg taaaatgttt
     5161 ccgggacgaa gacctgcgtg ccgaccggca gcctgaattc acccagattg actgcgaaat
     5221 gtcgttcata acccaggaag acattctgac catttttgaa ggattgatca aacatctgtt
     5281 caaaacggtt aaaaatatcg acatcgtaac actaccccgg ataacctggg ccgacgccat
     5341 gaaaaaatac ggcagcgata aaccggatat ccgtttcgga atggaattta ccgaattgac
     5401 ggacctggta aagggcaagg attttaaagt attcgatgat gccgagctgg tagtgggcat
     5461 caatgccgaa ggctgtgccg aatatacccg caagcagctt gacgagctta ccgattttgt
     5521 aaaaaagccg caaatcgggg ccggggggct tatttatctg cgttatgctg ccgacggaac
     5581 gctaaagtca tcggtcgaca aattttactc ccccgatgac ctgcagaaat gggcggaagc
     5641 ttttggagcc aagcccggcg acctgatgct gatcctggcc gggaaagcta tgaagacccg
     5701 caaagcattg aacgaactac gcctggaaat aggcggcaga ttaggattca gaaatccgga
     5761 aatatttgcc ccgctctggg tttacgattt ccctctgctc gaatgggacg atgaaacaca
     5821 gcgttattat gccatgcacc atccctttac ttctcccaag cccgaagata tcccgttgct
     5881 cgacaccgat ccgggtgccg tgcgcgccaa tgcttacgat atggtaatca acggcgttga
     5941 agtgggcgga ggctccattc gtatccatac caaggatatg cagcgtaaaa tgttacagtt
     6001 gctcaacttt agcgcggaag aagcgcaggc caagttcggc ttcctgatga atgcattcga
     6061 atacggcgcc cccccacatg gaggtatcgc ctttggtttt gaccgtcttt gctcggtgct
     6121 tggcggcagc gattccatcc gcgattatat cgcattcccc aagaacaatg ccgcccgcga
     6181 tgtgatgatt gattcaccat cgcgtatttc ggaggaacaa ctgaaagaac tgagcctgaa
     6241 gatctccatc tgatccagca ttgtatcaga tctgatagtc aatattttcc gacgccaaaa
     6301 ttcaggccct ggttatcccc ctgcccggta aacaggtacc cgttttattt ttcacagggt
     6361 cttcgccgat gggtacgaac aaagtcggtg tatcctgatg cccggatatc aacgcccgat
     6421 gaattttaca tgttctgtga accaccggta caacccggat atgcacataa tactaccctc
     6481 aaccgatccc atcaggaacc ttaacgaaac accggttata tatcaggctt tcacctgata
     6541 ggaataacag gcaactaatt ttcgcgtctc ttcctgcttt ttaaatattt atagacccct
     6601 gacacaaaat atgccaccag gatgaggtat atgattcctg tgaccggtgc gaccttctga
     6661 atggcgtatc ctgaaaagag cctgcatcca aaaagcgcca gcaacagcaa caaaccggcg
     6721 agcggaatgg gtgatttccg gtatttttcc atgtaatcgg gtttccagaa agccatgatt
     6781 atctcaatga taataatgac ggcacagaga caaattatag tcagcagttc cgttgttgtt
     6841 cccatattga ataaaattta cagatgagcg gctacttaat actttcttaa agttgttaaa
     6901 ccggcaaaaa aatccggatt agtaatcagt atccggaaat taaaaaacct ttacggaaag
     6961 tgtaacttcc aggacttctc agctttatcc ggcctcccgg tcagaactaa actccatcag
     7021 ttcgacaggc gccccgttgt gcaggatcat ggctactttc agccctggtg acggggagtt
     7081 gacttcgccc agcaattctt tatctatcag cgctgtttcc atatcgtcga ccacaaaggc
     7141 aacgtgcggg atttttttca cgagttcatg aaccttgcaa tgcggttcga accgcatgaa
     7201 ttctatcccg taagggctgt ttttaaatcc gccggcataa ataccgaggt gcgcgatatg
     7261 ttcttcgccc gggaaaggtt cattcacggg tatgccgata tgatgaaatt tccatcccca
     7321 ttccgaggtt gccaggggta attcatgatc tttccgggtg taattcataa cggtactgtt
     7381 tttataataa aatatgccct gtttcgattc gcctgaattt tcagtgacgc ttaaatgata
     7441 tgattctgta aagttgttac aaacttactg aaagaatcct ccaattgcat gctaaagcct
     7501 tttaaattct tactcatcag acaattggta tgtttaactt ttaaccgtgc aacaggcatc
     7561 aatgtgaaaa tgaaagaaat atcagccgcc gtaagcttta tgatatggat tagtcaggtt
     7621 gtgaagggta gctttcccga ccatgaaccg gaaagtgaat acatgaagag agtggtacaa
     7681 aaacggcttt caggatggat ttgccgtaga gatacctatg gtatgctgca aactgctgcc
     7741 gggcgctggt ttttgtgccg gcccagtgta aaatgaagag ggtggaaaat ccaccctgca
     7801 acaagtttaa atatgaatga tagaccctat gaatgtccta tttccggatc ttcttcggat
     7861 gaagaccatg gataatggta caacccatcg agcaggcgtt tttccttttg ttcgggcgac
     7921 agggattcgt atttctcttt ttcttcgtca atcctgcgct gtttttcggc ttcaacggca
     7981 tgaatttcgg tcaggtaaac gtcttccatg cgttttttca ggaagtcgtt ggccactgaa
     8041 gggaaaagtt caaggagcaa ttcctctttt tcattctgcg ccatttttac tccgccgtat
     8101 tcctcgtaaa tggggtttgc ctgttttttg taatatcttg aatcgtaggg aatttcttct
     8161 ttcaccccgg caattttata cctgaaatca ggatcgatag cgacaggtgt tttgccgtaa
     8221 cttcccttaa ccaggttgac atactgtatt gatttggtgg tatatatagg agctccttta
     8281 gattcgtcga tcgcgcaatt taccgcctgc acaccgacta tctggcttgt aggagtgacc
     8341 aggggagggc atcccgaggc aagccgcacc aacggaatca gttccatagc gcgggggagg
     8401 agtttttcaa gtttcagttg tttcaattgt gccagcatgt tgctatacat accaccggga
     8461 atttcggctt cgcggatgat atcgttggct tcaggcaggc caaaccaagc ttccagtcga
     8521 tggcatactt cgagcagttc ttcttctttg tcggctttgg catgggcaat ggccatctcg
     8581 accagttcgt cgatatcctt gggtaatttg tcttcaacga agttaaattc gcgcgggaaa
     8641 gcttttgtag cgtcaaaatc ggcgagttca cggcgtatct cccgcaattc caggttcagt
     8701 tgcaggacag cctgacgatc aacgccggtt tcaataccca tgcggtcgca gaacaactgg
     8761 atcagttcaa agggcggagc tgccgttcct ccggcaaaat tcatgatggc cgtatctact
     8821 atatcggcgc cgtttacaat agccatgagg gttgatgcta ccccgtaacc cggtgtgcag
     8881 tgggtatgga aatcgagggg aattgacagg gattccttca gcatacggac aagtcgtcct
     8941 gttttccccg gagtagccag gccggccatg tccttgatgg taatcatgtc ggcgccttcc
     9001 tgttcaagtt ccctggcttt gttcacaaaa taggcctttg taaatacatt ggtaggcagc
     9061 attttagcat ggaggagagc gcgcaggcgt tcgcgtttgg aatatttcgg gtctacggta
     9121 taaacaatac agcaatcggc aacgcctccg ttttccttca caaaacggat ggttgctttc
     9181 atgtttgaaa tatcgttgag ggcgtcaaaa atacgcatga tgcttacgcc cgattttata
     9241 gcgtttcggc agaaaccttc tattacttcg tcgggataag gattgtagcc aaaaagattg
     9301 cgtccgcgtg aaagagcagt caacaaggat ttattaccga tcccgtcccg gattttttca
     9361 agcctgtccc acgggtcttc gttaagatag cgcattactg aatcgggaac agccccgccc
     9421 catacttcca tagcataaaa tccagcctgt ttaaacaacg gcaataccct gtcgacctgc
     9481 tgctgggaca tgcgggtggc aaaaagcgac tgttgcccgt cgcgcagcgt aacatcacga
     9541 atcagaagac gtttattcat ttacattcca attaatcatc cgtttgcact gggaaaacga
     9601 accggttgtg ataatttttg aatacaattg ttaatcagtt aacaattaaa actgttatcc
     9661 gattcacagt acaaaagtat taatattacg tggatcaggc atatatttat ctttgaatat
     9721 ttacgataaa aggcagtttg tctccgggca tgagacattt aagccgggaa aggattcttg
     9781 ttccgaaatc actaatttgt atacctttat tgcattaatt cttacaacac gttttaatcg
     9841 atacatacca tgaaaaagta ttacctgatg acggctttcg tggcaatagc cgttattttg
     9901 aacacaggct gcaaacagcc cggtaacaaa cagacaaaca cccagctcag cgagatgcaa
     9961 caaaaagtga atgaatatgc cgtggtgaga ctgactactg acctcagcaa actcaccgac
    10021 aaggaaaagc aaatgatccc gatcctggtg gaagtttcag aaatcatgga tgacattttc
    10081 tgggaacaaa cgctcgggaa taaggcagct tttttcgata ctattaaaga tgaagctacc
    10141 aggcagttcg ccatgatcaa ttacggcccc tgggaccgcc tggatgagaa taaacctttc
    10201 attgccggta tcggtaaaaa agctccgggc gccaatttct acccggccga catgaccaag
    10261 gaggaatttg agaaatggaa taataaagac aaggcaagcc agtacacgct gatccggcgc
    10321 aatgccgacg gcagcctgaa atgcgtctgg tatcatgaag cttatgccga taaaatcaaa
    10381 aaagcatctg atctccttct caaagcagcc gcactggccg aagatcccgg attgaaaaag
    10441 tactttgaac tgcgctcgaa agcgttgctg accgatgatt attttgccag tgacatggcc
    10501 tggatggata tgaagaataa taccatcgat tttgtagccg gccccatcga gaattatgag
    10561 gatgagcttt tcggttataa aaccgcccag gaagcagcta ttctggtgaa ggataaagat
    10621 tggagcaaaa agctcgaaaa gtacgctgct atgttgcctg ccctgcaaaa ggagttgccc
    10681 tgtgacccga aatacaagaa ggaagtgccc ggaaccagtt ccgacctgaa tgcttatgac
    10741 attctatatt atgccggcaa cagcaattgc ggaggaaaaa ccattgccat caacctgccc
    10801 aacgacgaaa aagtgcaatt ggcaaaagga agccgccgcc ttcaactgaa aaatgccatg
    10861 caggccaaat tcgacaagat acttgtcccg attgccgatg tgctcatcga tccgtcgcaa
    10921 agggcaaata tcaaattcga cgctttcttc ggcaatgtga tgttccacga agtagctcat
    10981 ggcctgggca tcaaaaatac cgtcaatgga aaaggcaata tccgtgaagc gttgaaagag
    11041 cagtattcag gatgggaaga agggaaagcc gatatcctcg gattgtacat ggtaaccaaa
    11101 ctgattgaaa aaggggaact caaaggcgtt acagttgaag atgcatttgt tacttacatg
    11161 gccggactaa tccgttcagt ccgttttgga gccgccgaag cccacggaaa agccaatatg
    11221 atgtgtttta acttctttga ggataaaggc gctttcaaac tcagtgccga cgggacctac
    11281 aaagtggata tggaaaaaac ccgcaaggct atgaatgaat ggagtgcctt tattttgaag
    11341 atagaagggg aaggcgacta tcagcaggct gtaagctata tggctgccaa cgggaaagta
    11401 cgcgacgaac tgaaaaccgg catcgaaaaa ctcaagtctg ccaacattcc caaggatgtt
    11461 gtctttgaac agggggtaaa ggtattgggc ctgtaaaagc ccgatacttt tttcggcagg
    11521 tgtaaaggcg atcttttaca ctgaattttt tgctgtactg taatcaggat ggtaccgtcc
    11581 ggaaattttc tcccgctgat gccggtgtca acctgagccg ctaccagtac agaacccatg
    11641 caaattgtat tcctccggat tcacggaaag gatagggtat gcacagaaaa agttcattcg
    11701 atcggttgac agatgcatca gaaaggcaaa aacaagcggt aaaaatcaac ggcaatacgt
    11761 gatcgttagg gcttattggg ggcttccccc ggtattaacg gcttcgcagg tttaacgaat
    11821 atgttctgtc agatttcggt caggctgaag aaatcgttat atgaaatggg gttgttggta
    11881 ttaccggttt cggtaaatga ccattctatc tggtcaccgg ttttccagcc gggtttcagg
    11941 tcactgaggg agaaggtaca gatagcaata ttatggttca ggatatcctg atgcattacc
    12001 tgtccgttga cggtaatccg aatggaggat gtttcatagg cttcatacgg taacaggtta
    12061 taggtaagct caacaacaaa aacagtttcc gtattataaa ccttgtaatt tatggatttg
    12121 gggtggttgc cgttctttcc tttagctgaa ccgaccgtgt attgaaaact ctcaggttcg
    12181 gtaagatagt tttcggctgc attttcattt tgggaaacat tgctattcga aaattcagac
    12241 tcatctgttt gcagcgtatg ggatttttct tccctggagc atgagcacat tataccgttg
    12301 gtaatcacca gggcggctac aatcaatgct ttcagtattc ttttcatgac ctcggcattt
    12361 actttttaat caattcaaaa tcaggaatag agcgattttt taataaattc tgaataaact
    12421 acaaatacag aagtttcaaa atatatgcca tgttttatat aaaagtgcga cacagtcgtt
    12481 taagtagttt taatacgaaa ggcgttgtct caatttttta cgaatgggac aaaaaacaac
    12541 tgtaaccgct ataatatatt aaaccggaaa cctgtgtaaa ttcttacgga acgatcaggc
    12601 cggttgacag aatgatgtat ccgatacaca gagtaattag ggtatttaga agattgatga
    12661 aatcactttg tgacaattct cttacttttg tgacataatc attaatctca acatcgttta
    12721 tgaaaaaata cctattggca ttatgtatgc ttgtggccat atttgccaaa gctgccgaaa
    12781 cgcctgacga agggatgtgg ctacccatgt ttgttgaaag gctcaactat gtcgacatgc
    12841 agaaaatggg gcttcgtctc actcctgaag aactttacag cattaatcat tcaagtctga
    12901 aggatgctat cgtcagcctt ggcggatttt gcactgccga agttgtatcg cccgaaggat
    12961 tgctttttac caatcatcac tgcggttatg gtaatataca gaaacacagc acggtagacc
    13021 atgattacct gaccgacggt ttctgggcca tgagcaaagc cgaagaactt cctaacgaag
    13081 ggctgactgt ttcattcctt gaacgcatgg cagatgtaac caccgaagtg ctgggagttg
    13141 ttacttctga aatggatgaa caagcccgtc aaaaagctat cagcggaaaa attgacgaac
    13201 tgaaaaaggc tgcttcggaa gaagggaaat accaggttga attgaagagc ttttttaacg
    13261 ggaatgaata ttacatgttc gtttaccagg tttataaaga tgtcaggctg gtgggggccc
    13321 ctccgtcaag cattggaaaa tttggcggcg ataccgataa ctggatgtgg cctcgtcata
    13381 caggcgactt cagcatcttc cgcatctata cggcacccga cgggtccccg gccgagtatt
    13441 caaaagacaa tgtgcccctc aaacccaaac attacctgcc tgtttcctta aaaggatatg
    13501 aaaaaaacga tttcgccatg atatgggggt tcccgggcca gactgaccgc tatcgtactt
    13561 cctggggcgt ggatgcaacg cttaatgata tcaatcccgt tatcaccaaa ggccttggta
    13621 agattctcga aattcagaaa gccgggatgg atgccgataa cagtgtacgc attgcctatg
    13681 ccgacaacta tgccggactt gccaatttct ggaaaaataa agtaggggaa acccgcgacc
    13741 tgaaacgcct cggcgttgtc gctaaaaaac aggaactcga aaaacagttc gccgactggg
    13801 taaacgctac cgaagcccgc aaacaaaaat acggaaacgt actcagcact ttttccgatg
    13861 tgtacgccaa atacaaggat caaaatctgt attcagtatt atggcatact caaatgatcc
    13921 tgttcggttc acaggctttc agggcccccg gaatggctga aggacttgaa accgtattaa
    13981 agagcggcgt caagggagaa gaactggcaa aacaactggt gaaataccgt gagttttcgg
    14041 ccgaacaatt caaagaatac aacatcggca ttgagcagaa tgtcctggct gccgtcctgg
    14101 agatgttcta taccggtgtt ccgaaagagc agcaaccgga gattcttaaa acggttgaat
    14161 cgaaatacaa aggctgtttc catgcatatg ccgccgacct tttcaaaaaa tcgatctggt
    14221 atacccccga aaactttaat gcttttctcg ataaaccatc gttgaaaaaa ctgcagaacg
    14281 atccgaccgt taaactgtac agttcattca tggaaatttt caaaaaaatt caggagtcga
    14341 acaaggaatt atcgaaccag ctccgtgttg cccaaaggct ctttgctgcc ggacttcgcg
    14401 aaatggaacc cgacaagagc ttctatcccg atgccaacag cacgatgcgt atgacctatg
    14461 gcaaagtgct tgattactat ccggccgatg cagttcatta taaatattat accaccctgg
    14521 atggcgtaat ggctaaggaa gacccgacaa acgaagagtt tatcgttccg gccaggctga
    14581 aagacctcta tcagaaaaag gactacggcc agtacggtgt gaacggaacc atgtacacct
    14641 gttttctcac tgataacgat attaccggag gtaattcagg gagcccggtg cttaatgccg
    14701 atggccaatt gataggtctg gcattcgacg gcaactggga agccatgagc gggaatatat
    14761 tgtttgaacc cgaattgcag cgtactatct gtgttgacgt tcgctatgtg ctgtttatca
    14821 tcgataaatt cgccggcgcc accaacctca tcaacgaact tacgctggta aaataggaat
    14881 cattatctga cattataaaa aacaggcgcc cttttttacg g
//
LOCUS       DAOPYV010000069        14848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_4373_length_14848_cov_11.353546, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000069 DAOPYV010000000
VERSION     DAOPYV010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14848
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4373_length_14848_cov_11.353546"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="PKI35_09190"
     CDS             <1..1135
                     /locus_tag="PKI35_09190"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNX44596.1"
                     /translation="RIQIGDTVWAGNVEVHVQASEWYRHKHQQDRNYDNIILHVVWHN
                     DQQVYRASGEMIPTLELSGRIEEHIWKKYLYFMASRSWIPCESMVAGVDEFVKTAWLE
                     RLLVGRLERKSSFVEELLLASDHNYNEVFYRLLARNMGFKLNNEAFGILAGNLPYSYL
                     QKHADDLFQVEAMVFGQAGLLKGNFSDEYPVRLQQEYKFLKSKFSLKPMDGKVWRFSR
                     LHPGNFPTLRLAQFASIIHHSNGLFSKTIEAGDISSYALLFKSEASAYWKNHYVFDKV
                     AQARRKTLGADSINLLIINLVAPLLFVYGRHKGNAGLMAKPVELLGEINAEQNSITKR
                     WSNLGIGIASAAQSQALIELKTQYCDLKKCLSCGIGNALLRNS"
     gene            1210..3033
                     /locus_tag="PKI35_09195"
     CDS             1210..3033
                     /locus_tag="PKI35_09195"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNX44597.1"
                     /translation="MSTNKESWGTRVGLILAMAGNAVGLGNFLRFPMQAVQNGGGAFI
                     IPYLVCFLLLGVPLLFVEWATGRYGGQFGHHTTPLTFNRMGNRWFWKYIGVFGIFSNI
                     AIASYYCYIESWTLSYTIHSAIGSFNHLDQHGVAEFFNNYVDISTTTTGIPFEAIVFF
                     VLCLALNVWVLCRGLSGGVEKVAKIGVPLLIVFGVFLAVRGITLKAGSMGAAFDGTVG
                     LNFLWTPDFTSLTNPKVWLAAAGQIFFTLSLGQGSVQCYASYVKHKDDIALNAMSAGW
                     MNEFVEVVLGSAIIIPISIGYFGIDKVVELTRLGGLGLGFRIMPYLFAQWGTLLSAMA
                     GIAFFGLLFFAGITSSLAMGTPVMSFLMDEFNWKRKSSALAFGVAVLVLGLPTVLFFQ
                     QGVLDEYDYWAGTVSLVLFATFEMILFSWVFGMKKGWKEITHGADIKIPVVYRYIIKW
                     ITPFMLLAVFLGSLVRPANDEWNRISLKGWKLHNESIIGQLKHQGIGPNKSYFSDKFF
                     AERDGQVDSVYVLNGSKYICVTDTAGGIKTPLRYKIMDRDIPAVHCGDFLHTGDVLYT
                     GSVVNRVFYIDLARILLLSVFVVIGVLVYMAFRKRKKSGIC"
     gene            3092..3220
                     /locus_tag="PKI35_09200"
     CDS             3092..3220
                     /locus_tag="PKI35_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44598.1"
                     /translation="MNTTALLFMIISEFAITFLTVYFFVKVLRSDKKGEPDSYEEE"
     gene            3256..4695
                     /locus_tag="PKI35_09205"
     CDS             3256..4695
                     /locus_tag="PKI35_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNX44599.1"
                     /translation="MEQTGFRKSLNLIDSTAIVAGSMIGSGIFIVSAEMSRNLGSPGW
                     LLVAWAITGVMTIAAALSYGELASMMPKAGGQYVYLREAFNPLSGFLYGWTFFLVIQT
                     GTIAAVGMAFAKFFGVLVPWFSEKNILLDLRIVKVSAVHVLAIGSILILTCNNLLGIR
                     NGKWVQNIFTVLKAVLLLAFILLGLFVVRNTAAIQANASFFWKPQSATGEALSGWALI
                     AAIGVAMVGSLFSSDAWNNITFAAGEVKNPKRNIPVSLALGVIIVTVLYLLANFAYLN
                     ALPLRGNSGGQSVSDLGMQYAVNDRLGSAMIYALFGPLASILMAGFVVISTFGCNNGL
                     ILSGARVYYAMANDKLFFRQTGELNRFGVPGKALVIQAVWASLLCLSGTYSNLLDYVV
                     FAVLIFYVLTIAGIFRLRKTKPDAERPYKAFGYPVVPVIYMASAILIMMILLIYRPVY
                     TWPGLLIVLTGIPVYFLWRKFSPAFENCG"
     gene            4760..5686
                     /locus_tag="PKI35_09210"
     CDS             4760..5686
                     /locus_tag="PKI35_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNX44600.1"
                     /translation="MFKQIQDYFDFNRAERTSIVILGAVLIVLFFVPAFLRHNRKTEN
                     ADFKSFEKEIAAFEKSLVYNADNSGESPGHKIDFEHVDRSIAEQELTPFPFNPNEMTP
                     ENWRRLGLKEWQVKIITNYTSKGGKFFKKEDLQKIYGISRSQYEVLAPYITIPEREYP
                     TKRAGTRNPEASYAKLTIVDLNSADSATLVTLNGVGPSFARRIIKFRTLLGGFYSTSQ
                     LLEVYGFDSARYEKVVRYCKADPSNIKKINLNAVTTEELRKHPYFDYYLAKAIVDRRI
                     IGGKYTSIDQVAQLPLVHSDLFNKIKYYLALE"
     gene            5690..7063
                     /locus_tag="PKI35_09215"
     CDS             5690..7063
                     /locus_tag="PKI35_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX44601.1"
                     /translation="MNRSDILRKIFLPALLVLLVSLSYPGTAQDESESQLKKKLPQVS
                     GAQKVYVLNELSKSVRDKDRKQSFRYADDARRLAMKINDKPGLALAYKNIGNAYLFLA
                     SYDSALRYYHLALPIFRSVSDIVGVSACLNNIGVICSETGKYDEAIDYFYQSVEADRK
                     LGDEEGASITINNIAGIYKYQGKYALAVKLLNEQIPIDRKFKYVSGLIDKLNTLGVIY
                     VALSEPDKALKYYFEALHLAERDSNEYKRALLMNNIGNAYIVKNEYAEALPYLNLSLI
                     VSEEIDDQENIAQALTNLAEINKAQGNYFKANEQLMQVLKINESIDDKRTVAATLTCI
                     GDNLLWQKEFNKAIGYFMQSLKIADSIGATTEILDNYSKLVKAYSAISSFKTADSFQN
                     LYSDLYSRFKTDADTSKLIAVSQKTGSDQKIPVSGSWFSMERLIIFIGIFFIFISLLV
                     LIRKKRQ"
     gene            7150..8487
                     /locus_tag="PKI35_09220"
     CDS             7150..8487
                     /locus_tag="PKI35_09220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNX44602.1"
                     /translation="MAEITETAQVRKGHPPALYLLSATAAGERFAYYGMRALLMLYMI
                     AGVSEALPGMAFSKSTSGLVYGTFNALCYGFPFFGGIIADRLIGKRRSATIGGILIAA
                     GLIVLSLDNSLVPFISGLTLMAVGNGFFKPTVVTMIGELYEQGDPRRDNAFTLYYQLF
                     NGSVFFAPLLCGWIAVEYGYRAGFIVAGGAVLISMLIYWMMAPKMLGNIGKSVVVKQE
                     KAGKAGEKIALSKEEKDRIAVIFTLFFFVTFFWTGFEQAGSSMTVFTEESINRTIGNF
                     TIPTPWFQSLNPLLVFGLGFVFSALWMKLARIGKNPSTPVKMGMGMIFLGIGFVIMLG
                     AVAQLNTGTAKASMLWLLVAYLFHTFGELMLSPIGLSMVTKLAPAHMVSMFMGVWFLP
                     TFIAHLLGGVLASFIETLGPGLIFGGIAAFVIILGVSVFFLSKKLLYMMHGRD"
     gene            complement(8522..9265)
                     /locus_tag="PKI35_09225"
     CDS             complement(8522..9265)
                     /locus_tag="PKI35_09225"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNX44603.1"
                     /translation="MTELPEKQKIQKSLVVPIILLGMIWGIKLFEIIFQTDFSDFGIL
                     PQTLTGLRGIIFSPLLHADASHAFANTLPLFILTTALYYYYGRLANRILILSWLITGF
                     WVWAFAKNTGYHIGASGIVYALAAFHFTSGLLRREPRMMAFSLLVVFLYGGLVWGLYP
                     DFYPEKNISWQSHLLGSLAGIILAFFYRQNGPQRKKYSWEDEEDEDDDGELPDDENQD
                     PAIDKETTAGSDPTVVYHLRQRQHHEDQF"
     gene            9478..11097
                     /locus_tag="PKI35_09230"
     CDS             9478..11097
                     /locus_tag="PKI35_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX44604.1"
                     /translation="MKSDLLFRKILAVLMMIVLPVALNAQYSNRMSDQKETTQKYATL
                     MQLISYFYVEDVDESNLTEQAIIQVLKDLDPHSVYIPKDEVQRTNEPLQGNFDGIGVQ
                     FQIYHDTIVVVSPVVGGPSEKLGIQAGDKIVKINGEDAVGSKINNQYVFDRLRGPRGT
                     KVNIGIYRKNRKDIIDYTIVRDKIPLNSIDATYMVTDEIGYVKLSRFARTSKDEFVEA
                     VAELKKRGMKDLILDLRNNTGGYLDIAFELADQFLSANKLIVYTEGISSPRQDFNSTS
                     QGVFETGKLVIIIDEASASASEIVSGAVQDWDRGIIIGRRSFGKGLVQRPFMLPDSSM
                     VRLTTARYYTPSGRCIQKPYTPGSDEYYAEVYNRFKHGELVHSDSIKFPDSLRYFTHN
                     KRLVYGGGGIMPDIFVPIDTSFFSLYYTDALRKGVLNDFIMQYVDANRKELLRTYPDI
                     SSFMKGFRKDQELLNDFVEFGSKNALPRDDKGLEISGKQITSVLKGLLARNLYNVSAY
                     FEVIGKDDDDLKQAVQVIQNESLFRKLTLAN"
     gene            11395..12381
                     /locus_tag="PKI35_09235"
     CDS             11395..12381
                     /locus_tag="PKI35_09235"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNX44605.1"
                     /translation="MADISTKYLGLHLKSPMIIGSSGLTGSLENIIEYEKQGAGAVVL
                     KSLFEEQIMHEIDRTMGSGESDNQYPEAFDYISNFSRANSIEKYLTLIRGCKDSVKIP
                     IIASINCATAAEWTNFARKIQDAGADALELNLFVLPSDPGIAPGQYEKTYFEILDRVV
                     KQLSIPVALKISSYFSGLAHSAQKLSWAGAKGLVLFNRFFSPDIDIENLEIKATHLLS
                     SPEEIYMPLRWIAVLSDKIRCDLAGTTGVHDAGGMIRMMLAGAKAVEVVSAVYKNGSM
                     HIGKMIRELEQWMDKHHYTSTSDFIGKMSIGNAINPVAYERVQFMKHFSGIE"
     gene            12471..13430
                     /locus_tag="PKI35_09240"
     CDS             12471..13430
                     /locus_tag="PKI35_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX44606.1"
                     /translation="MPLVNSPGKVQVKGSRPVVFAVIAMLFWGMSFVWFKQICGFYGP
                     ITIIFLRLLISGSLLMLYIKLSHINTAIRSSDIKWFLLLAFTQPFCYFMGESFGLTKV
                     SSTISSVIIATIPLFAPIAAYYTFKEKVSGNTIAGIIISMTGIVVMLINPDLSLNASP
                     AGVGLLFFAVFSAVGYSLVIRKLADNYPVFIIIAMQNIIGAVYFLPFFLIFDLKQFLS
                     IRPPVSALIALVELAFFASTLAYLFYIVSIRDLGIIRANVFSNLIPVFTGIASYFLLS
                     EEFTGSKVIGMILVMAGVVFSQQRRFPGNLIRYSGKNSPPKTY"
     gene            13468..14637
                     /locus_tag="PKI35_09245"
     CDS             13468..14637
                     /locus_tag="PKI35_09245"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNX44607.1"
                     /translation="MNAKETKSKSLRIIVLGAGLVGGPVAAKLSEDPNFSVTIVDIDP
                     TVLDKISSKYKVTTLRIDLSDAKRVRKLVAGYGLVVSAVPGYLGNMVLRACVEAGKDI
                     VDFSFQEENFLEVDELAKEKGVTAICDMGLAPGTSNLLAAYACRQLDKPESLKIYVGG
                     LPRRHSWPFEYKAVFSPIDVIEEYTRPARFVKNGHLVVKPALSDVENIEIEGIGTLEA
                     FNTDGLRSLILNLKVPDMVEKTLRYPGHLYKIEVLRDAGFFGCQPINVAGNMIRPIDV
                     TSKLLFPILRFKEGEEDLTIMHIIAEGYKGSKHYRYTYDLFDTYDPETQIHSMARTTG
                     YAAVMAVRLIANGMYTDKGVSAPEYLANDEKCVTFFLNGLREFGIDYKLTVEEIR"
BASE COUNT         3980 a         3106 c         3588 g         4174 t
ORIGIN      
        1 ccgtattcaa atcggggata cggtttgggc aggtaatgtt gaagtgcatg ttcaggcttc
       61 ggaatggtac cggcataagc accagcaaga ccggaactac gataatatta ttttacatgt
      121 ggtatggcac aacgaccagc aggtttaccg ggcaagcggt gagatgattc ccacgctgga
      181 actttcgggc aggattgaag aacatatctg gaaaaaatac ctttatttta tggcttcgcg
      241 aagctggatc ccatgcgaaa gtatggtggc cggcgtggat gaatttgtga aaacggcctg
      301 gcttgaaagg ctgctcgtcg ggcgactgga acggaaatct tccttcgttg aggaattgct
      361 gctggcatcg gatcataatt ataacgaggt tttttatcgc ctgttggcgc gcaatatggg
      421 ctttaaactt aacaacgaag ccttcggaat tctggccgga aacctgcctt attcatacct
      481 tcaaaagcat gcggacgacc ttttccaggt agaagccatg gttttcggtc aggccggcct
      541 gctgaaagga aatttctccg acgaatatcc tgtccgtctg cagcaggagt acaaattcct
      601 gaaatcgaag ttttcattaa aaccgatgga tggaaaagtg tggcggtttt cacgcttgca
      661 tcccggcaat ttcccaacgt tgcggctggc ccagtttgct tccattattc atcactccaa
      721 cgggctgttt tcgaaaacga ttgaagcagg ggatatttct tcctatgccc tcttattcaa
      781 aagtgaagcc tcagcctatt ggaaaaatca ttatgtcttc gataaggttg cacaggcgag
      841 gcgaaaaacg ttgggcgccg attcgattaa cctgttaatt atcaatctgg ttgcgccgct
      901 gttgtttgtg tatggccggc ataaaggcaa tgcaggcctc atggcgaaac ctgtggaatt
      961 gcttggtgaa ataaatgcag agcaaaactc aatcacaaag cggtggagca atctgggaat
     1021 tggcattgca tcggcagcac agtcacaagc cctgatagaa ctgaaaacac aatactgcga
     1081 tttaaaaaaa tgcctttcgt gcggtatcgg caatgcgctg ttgagaaata gctgatattg
     1141 tctgctgaaa ttgaattttc acataactca ggtcaacact aaataaaaac taataacgat
     1201 taactaaaaa tgagtacaaa caaagaatca tggggaaccc gcgtagggct tattcttgct
     1261 atggcaggca atgcagttgg gctgggaaat ttcttacgtt ttccgatgca ggctgttcaa
     1321 aacgggggag gggctttcat tatcccttac cttgtatgtt ttcttctgct tggcgttccc
     1381 ctgttattcg ttgaatgggc taccggccga tatggtggtc agttcgggca tcacaccacc
     1441 ccgttgactt ttaacaggat gggcaaccgg tggttttgga aatatatagg ggttttcgga
     1501 attttttcaa acatagccat tgcttcatat tattgttata tcgagagctg gacgctgagt
     1561 tatacgatac attccgctat cggatcgttc aatcatctgg accagcatgg cgttgccgaa
     1621 tttttcaata actatgtcga tatcagcaca actacaacgg gaatcccgtt tgaagccata
     1681 gtgttctttg tgctttgcct tgcattaaat gtgtgggtgc tttgccgcgg attgagtggc
     1741 ggcgttgaaa aggttgcaaa aatcggagtg ccgttgctta ttgtgttcgg ggtttttctg
     1801 gcagtcaggg ggattacgct gaaagccgga agcatgggcg ctgcttttga cggcacagta
     1861 gggctgaatt ttctttggac acccgatttt acttccctca ccaatcccaa ggtatggctg
     1921 gcggctgccg gccagatatt cttcacgctc tcgctgggac aagggtcggt acaatgttat
     1981 gcctcatatg ttaagcataa agacgatatt gccctgaacg ccatgtcggc aggctggatg
     2041 aatgaatttg tagaagtggt gctggggagc gccatcatca tccctatttc aataggttat
     2101 ttcggtattg ataaagtggt ggaactgacc cggcttggcg gcctcgggct tggtttccgt
     2161 attatgccgt acctttttgc ccagtgggga acgttgctgt cggcaatggc aggtattgca
     2221 ttctttggtt tgctgttctt tgccgggatc acaagctcgc tggcaatggg aacgccggtg
     2281 atgtcgttcc tgatggatga gtttaactgg aaacgaaaaa gttcggcatt ggctttcggc
     2341 gtggctgtcc tagtacttgg cttgcctacc gtgttgttct tccagcaggg agtgctcgac
     2401 gaatatgatt actgggcggg aacagtttca ctggtattgt ttgcgacatt tgaaatgata
     2461 ttattctcat gggttttcgg aatgaaaaag ggatggaagg agattaccca tggcgctgat
     2521 atcaaaattc ccgttgtata cagatacatt atcaaatgga ttacgccctt catgctcctg
     2581 gccgtatttc taggctccct tgtcaggccg gcaaacgacg aatggaacag gataagcctg
     2641 aaaggatgga aattgcataa tgaaagtatt attggccaat taaagcacca gggaatcggc
     2701 cctaataaat catacttctc ggataaattt tttgccgaac gtgacggaca ggtcgactcg
     2761 gtatacgtcc tcaatggttc aaaatacatc tgtgtcaccg atacggccgg ggggataaaa
     2821 acgccgctcc gttataaaat catggatcgt gatattccgg cagtgcattg cggtgacttt
     2881 ttacataccg gcgatgtgct gtacaccgga agtgttgtga acagagtatt ttatattgac
     2941 ctggcccgca tccttttatt gagcgttttt gtggtgattg gagtactggt ttatatggcc
     3001 ttccgcaaaa gaaaaaaatc ggggatatgt tgaaaccatt atgcagcccc gttaattaat
     3061 tggtaacgtt aaagataaaa ttatgcaacc tatgaataca acagcccttt tgttcatgat
     3121 catctccgaa tttgcgatta cgtttctgac ggtttatttc tttgtgaaag ttcttcgcag
     3181 cgataagaaa ggggaacctg attcctatga agaagaataa tccctcgcaa ccattaaggg
     3241 caaaaaaatg atgttatgga acagaccgga ttccgtaaaa gcctgaacct gatcgattca
     3301 acagctattg ttgccggttc aatgatagga tcggggattt ttatcgtaag tgccgagatg
     3361 tcccgtaatc tcggttcacc gggatggctg cttgtagcct gggctattac cggcgttatg
     3421 accattgccg ctgcattgag ttacggcgaa ctggcctcca tgatgcccaa agcaggaggg
     3481 cagtatgttt acctgcgcga agcttttaat cccttaagcg gctttttata tggatggaca
     3541 ttttttctgg tcatacaaac gggtacaatt gcagcggtag gtatggcatt tgcgaaattt
     3601 ttcggagtac tggttccctg gttttcagaa aagaatatac ttcttgacct gcgaattgtt
     3661 aaggtaagcg ccgtccatgt actagccatc ggatccattt tgatactcac ctgcaacaat
     3721 cttctcggca tacgcaacgg aaaatgggtc cagaatattt ttaccgtatt gaaagcggtt
     3781 ttactgctgg ctttcattct cctcggactt tttgtggtgc ggaatacggc agctatccag
     3841 gcaaacgcct cttttttctg gaaaccgcaa tcggcgacgg gtgaagccct ttcaggatgg
     3901 gcgcttattg cagccatagg tgttgccatg gtgggttcgt tgttttcgtc ggatgcatgg
     3961 aacaacatca cttttgccgc cggggaggtg aaaaatccca aacggaatat tccagtaagt
     4021 ctggcgttgg gcgtcatcat agtcacggtg ctttacctgc tggcgaattt tgcttatctc
     4081 aatgcgttgc ctctccgcgg gaattccggc gggcaatcgg tctccgatct tggcatgcaa
     4141 tacgccgtaa acgacaggct gggaagcgcc atgatttacg cgctgtttgg gccgttggct
     4201 tccatcctca tggcgggttt cgtagtgatt tccactttcg gctgtaataa cggattgatc
     4261 ctttcaggcg ccagggttta ttatgccatg gctaatgaca aattattctt caggcagaca
     4321 ggggaattga acagattcgg agtgccaggc aaagccctcg ttattcaggc ggtttgggct
     4381 tccctgttat gcctgtccgg cacttactca aacctgctgg attatgtggt ttttgcggtt
     4441 ctgatctttt atgttctgac cattgccgga attttcaggt tgcgcaagac aaaacccgat
     4501 gcggaaaggc cttacaaggc attcgggtac cctgttgttc cggttattta catggcttca
     4561 gccatactga tcatgatgat cctgcttatc tacaggccgg tatatacctg gcctggatta
     4621 ctcattgttt tgaccggtat accggtttat ttcctctgga gaaagttttc acccgccttt
     4681 gaaaattgtg gatgatgctt acaagacgga cctgatcatt tttttgaaaa gattagtcgt
     4741 gttttaatct attttgcgaa tgtttaaaca gattcaggac tatttcgatt tcaaccgtgc
     4801 cgaacgcacg agtattgtga ttctgggcgc cgttcttatt gtgctctttt ttgtcccggc
     4861 ttttctaagg cataaccgta aaacggaaaa cgcagatttt aaatcgtttg aaaaagagat
     4921 cgcagcattt gaaaaatccc ttgtttacaa cgccgataat tccggggaga gtccgggcca
     4981 taagattgat tttgaacatg tcgaccgttc aatcgccgaa caggaactga cccctttccc
     5041 gttcaatccc aacgagatga cgcctgaaaa ctggcgccgg ctgggactga aagagtggca
     5101 ggtcaaaatc attacaaatt atacctcaaa aggcggaaag ttttttaaaa aggaagactt
     5161 gcagaaaata tacgggatca gccggtcgca gtatgaagtt ctggcgccat acatcacaat
     5221 tcccgaacgt gaatacccaa cgaaaagggc cggtacgcga aatccggaag cgtcatatgc
     5281 caaattgacc atagtggatt taaattcggc tgattcggcc actttagtga ctttaaacgg
     5341 ggtagggccg tcgtttgccc gcaggatcat caaattcagg acgctgctgg gcggattcta
     5401 ttccacatca cagttactcg aggtatacgg ttttgattca gcccgttatg aaaaagttgt
     5461 cagatattgc aaagccgacc cttcgaatat caaaaagata aacctgaatg ccgtcactac
     5521 cgaagaatta cggaaacacc cgtactttga ctattacctg gcaaaagcca tcgtcgacag
     5581 gaggatcatc ggcggtaaat atacttcgat tgatcaggtc gctcaacttc ccctggttca
     5641 ttcagacttg tttaacaaaa taaaatacta tcttgccctg gaataagcta tgaacagatc
     5701 agatatactt cgtaagatat tccttcctgc attgctggta ttgctggttt cgttatcata
     5761 tccgggaact gcgcaggatg aaagcgaatc gcaactaaag aaaaaacttc cgcaggtttc
     5821 aggggctcaa aaagtttatg ttctgaatga gttgtcaaaa tcggtaaggg acaaggacag
     5881 gaaacagtcg tttcgctatg ccgatgatgc caggaggctg gctatgaaaa taaacgacaa
     5941 accgggattg gccctagcct ataaaaatat cgggaatgct tatctgtttc tggcatcgta
     6001 cgattctgcc ctccgctact atcacctggc gcttccgatt tttcgttcgg taagcgatat
     6061 tgtcggggtg tcggcctgct taaacaatat tggggtgata tgcagcgaaa caggcaaata
     6121 tgacgaagcc attgattatt tctatcagtc ggtagaggcc gacaggaaac ttggcgatga
     6181 agaaggcgca tcaatcacta tcaataatat tgcgggtata tataagtacc agggaaagta
     6241 tgcgctggcc gtaaaattgt tgaatgaaca aatcccgatt gaccgaaagt tcaagtatgt
     6301 ctcaggcctt attgataagc ttaatacact gggtgtgatt tatgtagcac tttcggaacc
     6361 ggacaaggct ttgaagtatt attttgaagc cttacacctg gccgagagag atagtaatga
     6421 atataaaagg gccttgctga tgaacaacat cggcaatgct tatatcgtga aaaatgaata
     6481 cgcggaagcg cttccgtatc ttaacctttc actcatagta agtgaagaaa tcgatgacca
     6541 ggaaaacatc gcccaggccc tgacgaactt agccgaaatc aacaaagccc agggtaatta
     6601 tttcaaagcc aacgaacaac tcatgcaggt cctgaaaatt aatgaatcga ttgatgacaa
     6661 gcgcacggtg gctgctacct tgacctgcat aggcgacaat ttgctgtggc aaaaggaatt
     6721 caataaggca atcggttatt tcatgcaaag cctgaagatt gccgattcaa ttggcgctac
     6781 gacagagata ctggataatt attcaaaact ggtaaaagcc tattcggcaa tttcttcttt
     6841 taaaacagcc gatagcttcc agaatctata ctctgatctt tattcgcgtt ttaaaaccga
     6901 tgccgataca tcgaaactta ttgcggtatc tcaaaaaacg ggatccgatc agaaaattcc
     6961 tgtttcaggt tcctggtttt caatggaaag gctgatcatt ttcatcggga tattcttcat
     7021 ttttatttca ttgctggtat taatcaggaa aaagcgtcag tagcgtgcag aattccgggg
     7081 gatattaact gaaacggagc tccctgaaat caggaatatt ttgatatttt tggccaataa
     7141 aaaacttata tggctgaaat aactgaaacg gcgcaagtgc gcaaaggaca tccgccggca
     7201 ttgtatctgt tgagtgccac tgctgcgggc gaaagatttg catactacgg tatgcgggca
     7261 ttgctgatgc tatacatgat agccggcgtc tcggaagcct tacccgggat ggcattttcg
     7321 aaaagtacat ccggtcttgt ttacgggaca tttaacgcgc tgtgttacgg attcccgttt
     7381 ttcgggggta tcattgccga ccggctcatc gggaaacgga ggtcggctac aatcggagga
     7441 atcctgattg cggcaggtct tattgtcctc tcgcttgaca acagcctggt cccattcata
     7501 agcgggctaa ccctgatggc tgtaggtaat ggtttcttca agcctacagt ggttaccatg
     7561 atcggcgaat tatacgaaca gggcgatcct cgccgtgata atgcatttac tctttactac
     7621 caattgttca acgggtcggt attcttcgct ccgttgcttt gtggctggat tgccgttgaa
     7681 tatggttacc gtgccggttt tattgttgcc gggggtgctg tgctcatcag catgctgata
     7741 tattggatga tggcccctaa aatgcttggg aatattggaa agtctgtggt cgtaaaacag
     7801 gaaaaagcag gaaaggccgg ggagaaaata gctctttcaa aagaagaaaa agacaggatt
     7861 gctgttatct tcaccctgtt tttctttgtt acctttttct ggacaggctt tgaacaggcc
     7921 ggcagttcga tgaccgtatt taccgaagaa tcaataaacc ggacaattgg caattttacc
     7981 attcctacac cctggttcca aagtttaaat ccgttgcttg tcttcgggct tggatttgtg
     8041 ttttctgcct tatggatgaa actggcaaga attggcaaaa atccttcaac tccggtaaag
     8101 atgggaatgg gaatgatttt tctcgggatc ggtttcgtga ttatgcttgg cgcagtagcc
     8161 caacttaata ccggaacggc taaagcaagc atgttatggc tgctggtggc ttaccttttt
     8221 catactttcg gtgagttgat gctatccccc attggtttgt ccatggttac gaaactggcg
     8281 ccggcccata tggtttcgat gttcatgggt gtctggttcc tcccgacttt tatcgcgcat
     8341 ttgctgggtg gcgtattggc cagctttatc gaaacactgg ggccgggact tatttttggc
     8401 ggcatagccg cattcgtgat aattcttgga gtcagtgtgt ttttcctttc aaagaaactt
     8461 ctttacatga tgcacggcag ggattgagaa ttattccgcc gtacagacca ttacgggaca
     8521 gtcagaattg atcttcatga tgctgccgct gtcgcaaatg atagacaacc gttggatcag
     8581 atccggcggt tgtttcttta tcaatagccg gatcctggtt ttcatcatca ggtaactccc
     8641 cgtcgtcgtc ctcgtcttcc tcatcttccc aggagtattt ttttcgttgc ggcccattct
     8701 gccggtagaa aaacgccagg attatacccg ccaaagatcc aagcaggtgc gactgccagg
     8761 aaatattctt ctcgggatag aaatccggat agaggcccca aacaagtccg ccataaagga
     8821 aaacaaccag cagtgaaaaa gccatcatcc gtggctctcg ccgaagcagg ccgctggtga
     8881 aatgaaaggc agcaagggca tatacaattc ctgaagcgcc aatatgatac cctgtattct
     8941 tagcaaaggc ccaaacccaa aaacccgtaa tcagccagga gaggatcagg atgcggtttg
     9001 ccagccggcc atagtaataa tataaggcag tcgttaggat aaaaagcgga agggtgttgg
     9061 caaaggcgtg ggatgcatcg gcatgcaaca gaggggaaaa aatgataccg cgaagtcctg
     9121 ttaaggtttg cggcagaatc ccgaaatccg aaaagtcggt ttgaaagata atttcaaata
     9181 atttgatgcc ccagatcatt cccagcaaaa taatagggac aaccaggctc ttttgtattt
     9241 tctgtttttc cggtagttct gtcatagaga tgtgattact gacgcaaggt attcaaaggt
     9301 aaggccgaat gttaataaat gccaatgtgt cagggaaatt ctttcaatcg tgggatcagg
     9361 ccggcgggtt ttcgataaaa attgttaaac gaaaatcaat attgccccga tcaaatatcc
     9421 ggtcgattaa ttgtggtaat tttgaaactt catagtaatc taatccaaat cttcaacatg
     9481 aaatcagatc ttcttttccg aaagatattg gcggtgttaa tgatgatcgt gttgcctgtc
     9541 gcacttaatg cgcaatattc aaacaggatg agcgaccaga aagaaacgac gcagaaatat
     9601 gctaccttga tgcaactcat cagttacttc tacgtagaag atgttgacga gtcaaacctg
     9661 acagagcagg ccattatcca ggttcttaaa gatcttgacc cgcattcggt atacattccc
     9721 aaggatgaag tacagcgtac caatgaacca ctgcaaggca attttgatgg aatcggggtg
     9781 cagttccaga tttatcatga taccattgtc gtcgtatctc cggtagttgg cgggccctcg
     9841 gaaaaattag gtatacaggc tggcgataag atcgtgaaaa tcaacgggga agatgccgta
     9901 ggcagcaaaa tcaacaacca atatgttttc gacaggcttc gcggaccacg gggtaccaag
     9961 gtaaacatcg gcatctaccg aaagaaccga aaggatatca ttgattacac catcgtccgt
    10021 gataaaattc ctctcaacag cattgacgct acctatatgg ttaccgacga aatcggctat
    10081 gtgaaacttt cacgttttgc ccgcacctcg aaagatgaat ttgtagaggc agtcgctgaa
    10141 ctcaagaaaa gagggatgaa agacctcatt cttgacctca ggaacaatac cggcggttat
    10201 cttgatattg cctttgaact ggcagatcag ttcctgtcag ctaacaaact catcgtatat
    10261 accgaaggca taagcagtcc ccgtcaggat tttaactcta cgagccaggg cgttttcgaa
    10321 acaggaaaac tggtgattat cattgatgaa gcctctgctt cggccagcga aatagtttca
    10381 ggcgccgtcc aggattggga ccgcggtatt ataatcgggc gccgctcttt cggaaaagga
    10441 ttggtgcagc gtcctttcat gctgcccgac agttcgatgg tccgactgac tacggcacgc
    10501 tactatacgc cttcggggag gtgcattcag aaaccatata cgccaggttc cgacgaatat
    10561 tatgctgaag tttacaaccg tttcaagcac ggcgaacttg ttcattccga cagtattaaa
    10621 tttcccgatt cattgagata cttcacacat aacaagcggc ttgtatatgg cggcggcggt
    10681 atcatgcctg atattttcgt tccgattgat acttcatttt tttcactgta ttacacggat
    10741 gcgttacgca aaggcgtatt gaatgatttt atcatgcaat atgtggatgc aaaccggaaa
    10801 gagttgcttc gtacttatcc cgatatctca tcttttatga aaggattcag gaaagatcag
    10861 gagttgctga acgattttgt ggaatttggt tcgaaaaatg cgttgccgcg cgatgacaag
    10921 ggccttgaaa tttcgggcaa acaaattaca agcgttctga aaggcttgct tgcaagaaac
    10981 ctgtacaatg tgagtgccta tttcgaagtg atcggtaagg atgatgacga cctgaaacag
    11041 gctgttcagg ttattcagaa tgaatccctg tttcgtaagc ttacattggc aaactgacag
    11101 aaaatcattt cgttggtctg tcgatatccg gggtcttact ttctggtcag ccttatgtac
    11161 gcgtaggata aaaggcgtat ttctgaagca atatttcttt tcgttaaaaa agtgattaaa
    11221 catcaaataa tcgaattatt gataagatag ccatccaatc aatttagaat aattgatatt
    11281 tttacttccg gtatgtacaa agtattccgg gtatcgtgaa gcagctttag atgggatgct
    11341 tcctgtccgg ataacgggag cggttgatca ggtataaact taaaaatcag tacaatggca
    11401 gatattagta caaagtacct tggattgcat ctgaaaagcc cgatgatcat aggaagctca
    11461 gggctgaccg gttcgctcga aaatattatt gagtacgaaa agcagggcgc tggtgcggtt
    11521 gtgctcaaat cgcttttcga agagcaaatc atgcatgaaa ttgaccggac aatgggttcc
    11581 ggtgaatcgg acaaccagta tcctgaagcc ttcgattata tcagcaactt cagccgggcc
    11641 aatagtattg aaaaatacct cacccttatt cgcggatgta aggattcggt taaaattccc
    11701 attatagcca gtatcaattg cgcgaccgca gcagaatgga caaattttgc ccgtaagatt
    11761 caggatgcag gcgccgatgc ccttgaatta aatctgttcg tattaccgtc ggatccgggt
    11821 attgcaccgg ggcagtatga aaaaacatat tttgaaatac tcgaccgggt tgtgaaacag
    11881 ttatcgatac ctgtcgccct gaaaatcagc tcttattttt cagggctggc acattcggca
    11941 cagaaactta gctgggccgg ggctaaagga cttgtactgt tcaaccggtt tttttcgccg
    12001 gatattgata ttgagaatct ggaaattaaa gcaactcatt tactaagttc accggaagag
    12061 atttatatgc ctctgcgttg gatagctgtc ctgtcggata agatacgctg cgatctggct
    12121 ggtacaaccg gtgttcatga tgccggagga atgatcagaa tgatgctggc gggggcaaag
    12181 gccgtagaag tcgttagtgc agtatacaaa aatggcagca tgcatatcgg gaaaatgata
    12241 agggaactgg agcaatggat ggataaacat cattatacgt caaccagcga tttcatcgga
    12301 aagatgagta taggtaatgc aataaacccg gtagcctatg aaagggtaca gtttatgaaa
    12361 catttttccg gaattgaatg agtcggataa caggggtgtt tgggtattcc tgccaggcac
    12421 atagtaattc ctgacgacat tcagcatact gtatatctca tattattctt atgccgctcg
    12481 ttaattcacc agggaaagtg caggtaaaag gctcccgtcc ggtagtcttc gcagttattg
    12541 cgatgctctt ctggggaatg tcgtttgttt ggttcaaaca gatttgcggt ttttacgggc
    12601 ctatcaccat aatattcctg cggctgctga tttcaggcag tttattgatg ctgtatataa
    12661 agctgtcgca tataaacaca gccatccgta gcagcgatat caaatggttc ctgttacttg
    12721 catttactca gcctttctgc tatttcatgg gcgaaagttt cggccttaca aaggtttcat
    12781 cgacaatttc gtcggtgatc attgcaacaa tacccctttt tgccccgatt gcagcatatt
    12841 atacgttcaa agaaaaagtg tcaggaaata ccattgccgg tattatcatt tctatgaccg
    12901 gtatcgttgt aatgttaata aatccagacc tttcactgaa tgcttcacct gccggtgtcg
    12961 gattgctttt ttttgctgtc ttttcagccg tcggctattc gctggtcatc aggaaacttg
    13021 ctgataatta tccggtattc atcattattg caatgcaaaa cattatcggt gcggtgtact
    13081 ttcttccatt ctttctgata ttcgacctga aacagtttct gtccataagg cctcctgttt
    13141 cagcattgat agccctggtg gagctggcat tcttcgcatc aaccctggca tacctgtttt
    13201 atatagtatc tatcagggat ttagggataa tacgcgccaa tgttttttcg aaccttattc
    13261 ccgtttttac cgggatagcc tcttatttcc ttctttcaga agagtttacc ggttcaaaag
    13321 tgataggaat gatactcgta atggcaggtg tggttttctc acagcaaaga cggtttccgg
    13381 ggaatctgat ccgttactca ggcaagaatt cacccccaaa aacatactaa atacttaaag
    13441 caacttagat aactaacatt tccgatcatg aatgctaaag aaacgaaaag caaatcatta
    13501 agaattattg tattaggtgc cgggctggtc ggcggcccgg ttgcggcaaa attatctgaa
    13561 gacccgaatt ttagtgtaac aattgtcgat attgatccaa cagtcctcga caagatatca
    13621 tcaaagtaca aggttactac ccttcggatt gatttatcgg atgcaaagcg tgtcaggaaa
    13681 ttggtggcag gttacgggct ggttgtgagt gctgtaccgg gatatctggg taatatggtg
    13741 ttaagggcct gcgtcgaggc tggtaaggat attgtggatt tctcatttca ggaagaaaac
    13801 ttccttgagg ttgatgaact tgctaaagaa aaaggggtta cggctatctg cgatatggga
    13861 ttggcgccgg gaacaagtaa tctgctggct gcctatgcat gcaggcaatt ggataagccc
    13921 gaaagtttga aaatttatgt tgggggatta ccgcggcgtc attcctggcc atttgaatac
    13981 aaagctgttt tttcacccat tgatgtaatc gaagagtata cccggccagc acgcttcgtg
    14041 aagaatggcc atttggttgt aaaaccagct ttatctgatg ttgaaaatat tgaaattgag
    14101 ggaatcggta cgctggaagc tttcaatacg gatggtttaa ggtccttgat cttaaacttg
    14161 aaggttcccg atatggtcga aaaaacgctt cgttatccag gccaccttta taaaattgaa
    14221 gtattgcgcg atgccgggtt cttcggttgt caacctatca atgtggcagg gaatatgatc
    14281 cgcccgatag atgttacttc gaaattattg tttccaattc ttcggttcaa agaaggcgag
    14341 gaagacctta caataatgca tatcattgcc gaaggttaca agggtagcaa acattacagg
    14401 tacacatatg acctgttcga tacctatgat ccggaaacgc agatacattc catggccagg
    14461 actaccggat atgctgctgt tatggctgtg cggcttattg ctaatggcat gtacaccgat
    14521 aaaggcgttt ctgcacctga atatctggcc aacgacgaaa aatgcgttac gtttttcctg
    14581 aatggtttac gtgagtttgg aattgattac aagctcactg tcgaagaaat aaggtaaaaa
    14641 ttatcaaagg ccttttattt taaggaaaaa gtcctaattt tgcacccctg ttggcggggt
    14701 agctccgttg gttagagcgt cggattcata tccgtccgtc ggagagggac agactcgtgt
    14761 tcaacagtga ggaattgaaa ataaaatcat agaggcgggg tagctcagtt ggttagagcg
    14821 tcggattcat aacccggagg tctcgagt
//
LOCUS       DAOPYV010000070        14454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_4550_length_14454_cov_10.719425, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000070 DAOPYV010000000
VERSION     DAOPYV010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14454
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4550_length_14454_cov_10.719425"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            553..1794
                     /locus_tag="PKI35_09250"
     CDS             553..1794
                     /locus_tag="PKI35_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNX44608.1"
                     /translation="MTSQVLYYIILAIVVAGFLFGIVLDFLNSRTLNSRMPDAVKGIY
                     DEEKYRKQQDYQRVNYRFELISGAFSFILILAMLCFRGFAVLDQFVRAISDSAIWQTI
                     LFFGILGLAADLLATPFDLYQTFGIEQKFGFNTTTAYTYVFDKIKGWLLAGILGGGIL
                     AMIVWLYYRTGDWFWVYVWGIITVFTVFINYFYTVLLLPLFNKLTPLEPGSLYSDIDQ
                     FAKKTGFSLKSIYTMDGSKRSTKGNAYFSGFGKQKKVVLFDTLVADHPNNELVAILAH
                     EIGHYKKNHIFKGLALSFVQTGALLYILSLLVGSPVLSASLGSSFPGFHMGLLAFGIL
                     YSPVSEFLGIGMNAISRRHEYQADAFAAANHCGEDLQQALKKLSSKNLVNLLPHPFYV
                     FVNYSHPPLLERLKALQKFKP"
     gene            1911..4265
                     /locus_tag="PKI35_09255"
     CDS             1911..4265
                     /locus_tag="PKI35_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNX44609.1"
                     /translation="MLKELRNIKWPSFGKIVEVLRSSWKAVIDYFRIPEGVPLLVRGA
                     VVTAYVFAFFLLFILLVDINFLGLFGKSPVIRELKNPPMSITSELISADGKLLARYYT
                     EDRTPVEYKDLPQNLIDALIATEDVRFYKHHGIDFKATFAALWSTLRGDRRGGSTITQ
                     QLVKNLFKTRSDYSTGIVGKIPGLSTLNYKMKEWINALKIESYYSKEDIITMYFNTVD
                     FGSKAFGINSASSMFFNCHPIDLKPHQSALLVGILKAPSYYSPVSHPKKAFTRRNQVF
                     VQLERYGYISSAEKDSLSDLPLGVKFDRRNKKSDGASYLRDAVARSLQQWSRESGYDI
                     WTDGLKIYTSIDSHMQEYAEKSVGEHLRRLQQVFNRGGSSVVVPWIDENGKEDFGYFL
                     NLTDDVPAIKNAVIRWGNNADSLKYHLTKRHKMEVFTWNGVRDTTLSTIDSIKHYKRF
                     FQCGFIAMEPATGLVKAWVGGINYAYFKYDHVNQSKRQPGSLFKGFVYSAAFDNGFGP
                     CDHLTDQPVSIKYVEKGVNKEWKPRNVDRSHSGSMTLKHAFARSVNSIAVQITQKIGW
                     KKIIERAHALGIQSELADVPSVCLGSSDVSLAEIVAAYCGFINDGYRVDPVLVTRIED
                     KDGNVLYEAKSEKKRVISSENAFLMTTILRAGLTEPGGTPQGLFEHDIFRFDTDFGGK
                     TGTSNDYADGWFIGVTPGLVGGCWVGNDDRSIHFRTSHVGEGLRTAMPVYGKFMEKVL
                     KDEKMKRYRQRFGKPAVTISKSYDCHTVLPKADSLRLANDSIIE"
     gene            4317..4823
                     /locus_tag="PKI35_09260"
     CDS             4317..4823
                     /locus_tag="PKI35_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX44610.1"
                     /translation="MQIEKNTVVSLCYTLKADNSEGDIIEVAQETDPLVFLYGAGQML
                     PKFEENLANLKQGDPFEFGLLSTDAYGDHDPEAVIDLDIEIFKVDGKLDEEMVAPGNM
                     IPMRDSEGHMLQGKVVSISPVAVKMDFNHPMAGQNLHFSGSILEVRQATEEELNHGHV
                     HGCCGHDH"
     gene            complement(4919..5323)
                     /locus_tag="PKI35_09265"
     CDS             complement(4919..5323)
                     /locus_tag="PKI35_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44611.1"
                     /translation="MIEYVHNHITSELQQNARTDTIFILTSIALNLITLAVNWGMAEK
                     SRTNESTFVAMFLFVVLLVLVNLVVVIGLLKGKQTRRKLISGLMKIYKDKDVDKYYDE
                     SLLNNYSVRYNLFISVVAGTGLIAIVVPFVLR"
     gene            complement(5354..6010)
                     /locus_tag="PKI35_09270"
     CDS             complement(5354..6010)
                     /locus_tag="PKI35_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44612.1"
                     /translation="MRTILLSLILAALSMILNAQTWKVRVEKTTGSHRVKTLRIKPTM
                     QLTVGNQLPVTDTLKQYKYYYGEFISGSADSLQMKLKTITAHQLYTNGIHYTSVVPAK
                     QYYLSTPSADTNMMRIALSDIHSLDYSFEKWRGMSEIGEPVILGSLLVLIFSPLICYN
                     FSDGKINPESYRNWALGSTIGIVAGIGTAVAISAIFPHGRYQFLEGWPYKKAKVWKFR
                     "
     gene            complement(6062..6412)
                     /locus_tag="PKI35_09275"
     CDS             complement(6062..6412)
                     /locus_tag="PKI35_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44613.1"
                     /translation="MNSKIVNWSARILSLLSILFILIMSFDCIGGDYSLKEQLICFVM
                     HNIPTFTLIILIIIAWKWELAGGILFIVAAFAGSILFKVFTGNAAALILIAPILIAGV
                     LFVIKGEVLNRKPE"
     gene            complement(6543..7448)
                     /locus_tag="PKI35_09280"
     CDS             complement(6543..7448)
                     /locus_tag="PKI35_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNX44614.1"
                     /translation="MKNNSFFSALLFIVILAAGIVLAYSMKDTATSGTTTAVGIGSFL
                     LALIAASATKVADQWNRAIVLRLGKFHRLRGPGLFFIIPVIDAVPYWIDIRVITTTFK
                     AERTLTRDTVPVDVDAVLFWKVIDPKKAALDVAEYQSAISWASQTALRDVIGKTMLSD
                     MLEGRDKMSILLQKIIDERTEPWGITVISVEVKDVLIPSGLEDAMSMQAQAERERQAR
                     VILGDSERQVAQKFEEAAQTYADNPTAFHLRAMNMLYEGLKTNATIIVVPSTAVETMQ
                     LGSVAGLTALTKELAKEKSQNQPPA"
     gene            complement(7936..9762)
                     /gene="pepF"
                     /locus_tag="PKI35_09285"
     CDS             complement(7936..9762)
                     /gene="pepF"
                     /locus_tag="PKI35_09285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HNX44615.1"
                     /translation="MNSYSQKTRTEIPDNYKWNLTDIYKSDADWRASKESIVKKMDEV
                     VKFRGTLTRSASDLLRCMEYNSGIRKEANKLYLYANLNSDLDTRDMKYSGMKQELQQI
                     FSGFAAKSAFIEPEILSADWSLIDGFIKSEPRLAPYRMELDNMFRMKKHTLSEAEERI
                     LALSGPVGGVPESVYNTFSNAEMPSPEATLSDGTKITVTKPEYSRYRASANREDRQIV
                     FKAFWDNYDKFKASYGEMLYGNVQSHIFQAKARNYQSSLEASLYPSNIPVEVYRSLIS
                     NVDKSLPAFHRYLQIKKRMMNLDTLKYMDLYAPVVKDVDLKYSYEEASKIILEALKPM
                     GEEYVSTVKKAIDERWIDVYPTQGKQTGAYSDGSYYDGHPFIKLNYNGLYDDVSTMAH
                     ELGHTMQSYYSNKTQPFPLADYQTFVAEVASTFNEVLLFNYMLKNEKNDDVKLSLLMN
                     WLDGFKGTLFRQTQFAEFELKIHEAAERGEPLTGETFSKMYADIVKAYYGHDKGICKI
                     DDYIAMEWAYIPHFYYNFYVYQYSTSFTASISLAEKVMSGDKNALNKYIEFLSSGGSD
                     YPIDLLKKAGVDMTTSEPFEKTIQSMNKVMDEIEKILDKKSK"
     gene            10462..11643
                     /locus_tag="PKI35_09290"
     CDS             10462..11643
                     /locus_tag="PKI35_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44616.1"
                     /translation="MKNILRLFRWLPVLFLLLVSINRLTAQVTYDIYVYSLIDGTTVQ
                     ASVTPGYYEYNRSWSPNGKKLAHEECGFDINGNWYQNIYITEISSHETTPLAGGENGN
                     DAAWSPEGKKIMFDKVTDEWGYSGIFCVPDEGGDPTLLRNYGIMAEWSPNSHYIVFLD
                     EDTWWLKTMHLDDGDETFVVFGAENPDWSPNGQYIVYDEWWCCGGIWIIEVDHAGNPV
                     GDPVQLTSTGGQPTWSNNSKTIYYHDASPDGQYDIYSISVEGGTPELVCGLDIPDYGN
                     YDPCASNNGKYIAFAGATVPGDKSGPAGFQHDAEQESGLLVTVIGNPGAEGFKLNIRS
                     NSPESISLRIIDSEGRVVSDQTGIQPNSMLGIGNGYTPGIYLAEIRQGAQFKVIRLMK
                     Q"
     gene            11751..12227
                     /locus_tag="PKI35_09295"
     CDS             11751..12227
                     /locus_tag="PKI35_09295"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNX44617.1"
                     /translation="MKKAIISTFAAVIMLSVFTTVSAQEWTKAQKEVWQAVDNIWSNL
                     KAGNLDGFAAGFHEKYQGWNNYLPLPVGKQKNIASYRENKDIQKLESYEIEPARITVS
                     ENSAVVDYYFSITISEKKDGKSEVKVYSGKNVEFFVKEEGKWLLLGDMTYIKTDGK"
     gene            complement(12475..12894)
                     /locus_tag="PKI35_09300"
     CDS             complement(12475..12894)
                     /locus_tag="PKI35_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44618.1"
                     /translation="MKAKFIASGLFGGIINFFLGWLVYGKLLMPFLNANTIHYEGLTK
                     EMPVLWMLVISCLATGYFLAFIFKRWAGISTLKGGLIGGLIIGFFIAVMYDFSLMSMW
                     NLYNLKAILVDALATVVIYGITGAAIGWGLAVREKKE"
     gene            complement(12995..13441)
                     /locus_tag="PKI35_09305"
     CDS             complement(12995..13441)
                     /locus_tag="PKI35_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HNX44619.1"
                     /translation="MRIWSLHPKYLDGKGLIALWRETLLAKKVLEGKTKGYRHHPQLE
                     RFRQTENPPGMISRYLQSVYQEAVERGYHFDSSKFDAGDGDYRLSVTSGQMEYEYHHL
                     LNKLKIRDPERHRQFIKLTHIEPHPLFVVVEGKVEKWEITEPDRPA"
     gene            13625..14164
                     /locus_tag="PKI35_09310"
     CDS             13625..14164
                     /locus_tag="PKI35_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNX44620.1"
                     /translation="MNQKKSFSALALAGLALLTQSCASIPKGATVVKPFDKERYLGKW
                     YEIGRLDHRFERNLDNVTAVYSMNNDGTIKVTNSGYKTTTKEWKQVTGKAKPSGKPGE
                     GKLKVSFFGPFYGAYNIIALDEQYRYALVAGNNLKYLWILSRETSIPEDIKLQFLATA
                     KSVGYDTSSLIWVKHDNGK"
BASE COUNT         3911 a         3272 c         3354 g         3917 t
ORIGIN      
        1 ttgcagtaat cattgttctt cttggagcag aatggttcct gagaaaaaga gccggagagt
       61 attagaaggc tgggagccgg gagcagggag aacggagaac ggaaaacgga aaacggtgaa
      121 cgcaaacaca taacacagat cacagaacgt agaacacaga acatagaacg caaaacgcaa
      181 aactcaaaac gctaaaagtt tacctgcctg ggcaaggttg tgggtaagac ggcaggctaa
      241 cagccaacgg ccaaacaaaa ccggaatcct gaaaccctgc cccctcccac aaccttgcgc
      301 aaggcaggca ggcctgccat cttacgcgca gccttcccgg gcaggcaggc ctgaaaccct
      361 gaaaccctgg aaccctgaaa ccctgaaacc ctgaaactct gcacacgaat cacgagccac
      421 gaatttcacg aatgcaatca cgaattgcac gaatcacgaa caacgaatca cgaatcacga
      481 aacacagaac tcaaaactca aaactcaaaa ctcaaaactc aaaacactaa aagctaaagg
      541 ctaacagtca gtatgacctc acaagtcctg tattacatca ttttagcgat cgtcgttgcc
      601 ggttttctgt tcggcatagt tctcgatttt ctcaactcca gaactttgaa cagcaggatg
      661 cctgatgccg tgaagggtat ttatgatgaa gagaaataca ggaagcagca ggattaccag
      721 cgtgtgaatt accggtttga attgatttca ggtgcgtttt catttattct tatcttggca
      781 atgctgtgtt tcaggggatt tgctgttctt gatcagtttg tacgcgctat cagcgattca
      841 gcgatatggc agacaatatt atttttcggc attctgggcc tggcagccga cctgctcgca
      901 actcctttcg atctgtatca gacctttggc attgaacaaa agtttggctt taatactaca
      961 accgcataca cctatgtttt cgataagata aaaggctggc tgcttgccgg catcctcgga
     1021 ggtggaatcc tggccatgat cgtatggctg tattacagaa ccggcgattg gttttgggtt
     1081 tacgtttggg gcattatcac cgtttttacg gtttttatca attattttta taccgtactg
     1141 ctccttccgc ttttcaataa gctaactccg ctggaaccgg gcagccttta ctcggacatt
     1201 gatcagtttg caaaaaagac aggcttcagc ctgaaaagca tatacacgat ggacggatca
     1261 aaacgttcga ccaagggaaa tgcttatttc agcggttttg gaaaacagaa gaaagttgtg
     1321 ttgttcgaca cgcttgtagc cgaccacccg aacaacgagt tggttgctat cctggcccat
     1381 gaaataggcc actataaaaa gaatcatatc tttaagggat tggcgcttag ctttgtacaa
     1441 accggtgcat tgttgtatat cctttcgctg ctggtcggga gtcctgtttt atcagcctcg
     1501 ttaggttcat cattccctgg ttttcacatg gggttgcttg ctttcggaat tttatacagc
     1561 cctgtatcgg aatttctggg aatcggcatg aatgcaattt cccgccggca tgaataccag
     1621 gccgatgcat ttgctgccgc taaccattgc ggcgaagacc tgcaacaggc gctgaaaaaa
     1681 ctgtcgtcga aaaatctggt taacctctta ccgcatccct tctatgtatt tgttaattac
     1741 tcccacccgc ccctgttgga aagactaaag gcattgcaga aattcaagcc ttaatacaag
     1801 ccgctgattt acagccaaat aatctttaaa gaaagataat ccgaccgcgt atcccgtgga
     1861 ttgtttttgg ctacctttgc agatcatttc catgaaataa atccgattgc atgttaaagg
     1921 aattacggaa catcaaatgg ccgtcgtttg gtaagattgt tgaagtatta cgcagttcat
     1981 ggaaggctgt cattgattat ttccggatac ccgagggtgt cccattgctg gttcgcgggg
     2041 cggttgtaac ggcatatgtt tttgctttct tcctgctttt tattcttctt gttgatataa
     2101 acttcctggg gcttttcggc aaatctccgg ttatacggga gttgaagaat ccgccgatga
     2161 gtattacttc ggaacttatc tcggccgatg ggaagctttt agcgcgttac tataccgagg
     2221 atcgtacccc agttgaatac aaagacctgc cccaaaacct gatcgacgcg ctgattgcta
     2281 ccgaagatgt ccgtttttac aagcatcatg gcatcgattt caaagccacc tttgcggctt
     2341 tatggtcgac actccggggc gacagacgcg gtggaagcac catcacccag caactggtaa
     2401 agaacctctt caaaacccgg agcgattatt ccacgggaat tgtcggaaag atacccggct
     2461 tatctaccct gaattacaag atgaaagaat ggattaatgc gctgaaaata gaaagctatt
     2521 attccaaaga ggacatcatc acgatgtact tcaacaccgt agatttcgga agcaaggcgt
     2581 tcggcattaa ttcagcgtca tccatgtttt tcaattgcca cccgatagat cttaaacctc
     2641 atcagtcggc attgctggtc ggcatcctca aggctccatc gtattacagt ccggtatctc
     2701 atcccaaaaa agccttcacc cgccgtaacc aggtatttgt tcagttggaa agatatggtt
     2761 acatttcttc ggctgaaaaa gattctttat cagacctgcc gctcggtgtg aaatttgacc
     2821 ggagaaacaa aaaaagcgac ggcgcttcct accttcgcga tgctgtggcc cgttcgttac
     2881 agcaatggag ccgtgaaagc ggttacgata tctggactga tggtttgaaa atttatacct
     2941 caatcgactc gcacatgcag gaatacgctg aaaaatcggt tggggaacat cttcgccggc
     3001 ttcaacaggt ttttaaccgg ggaggatcat ccgtagtggt tccctggatc gatgaaaacg
     3061 ggaaagagga tttcgggtat tttctaaacc ttaccgacga tgtacctgct ataaaaaatg
     3121 ctgtcatcag gtggggaaat aatgccgatt cgctcaaata ccatcttacg aagcgtcaca
     3181 agatggaagt ctttacctgg aacggcgtcc gcgatacgac actcagcacc atcgattcaa
     3241 taaaacatta caagaggttc ttccagtgcg gcttcattgc catggaaccg gctaccggtc
     3301 tggtaaaagc ctgggtggga ggaatcaact atgcctattt caaatatgat catgtgaatc
     3361 agtcgaaacg gcagccggga tcattgttta aaggctttgt ttactctgct gcctttgata
     3421 acggatttgg cccctgcgat caccttaccg atcaaccggt ttcgatcaaa tacgttgaaa
     3481 agggagtcaa caaagagtgg aaaccccgga atgtagatcg tagtcacagc ggttccatga
     3541 cgcttaaaca tgctttcgcc cggtcggtga attccattgc cgttcagatt acacaaaaaa
     3601 tagggtggaa gaagataata gaacgggccc atgcattggg aattcaatcg gaactggcgg
     3661 atgtaccttc ggtatgtctc gggtcaagcg acgtttcgct ggccgaaatc gtcgcggcgt
     3721 attgcggctt tattaatgac ggctatcgtg tggaccccgt tctggttaca cgtatcgagg
     3781 acaaagacgg caatgtcctt tatgaagcca agtccgaaaa aaaacgtgtc atctcaagcg
     3841 aaaatgcatt cctgatgacg accatccttc gggcagggct gacagaaccg ggcggcactc
     3901 cgcaggggct tttcgaacat gacatcttca ggttcgatac cgatttcgga gggaaaacag
     3961 gaacctccaa cgactatgcc gacggctggt ttatcggcgt tacgccggga ctggttggcg
     4021 gatgctgggt tggaaacgat gaccgctcta ttcatttcag gacgtcgcac gtcggcgaag
     4081 gcctcagaac agccatgcct gtttacggga agtttatgga gaaagtgctg aaagatgaaa
     4141 agatgaagcg gtaccgtcag cgtttcggta aaccggctgt tactatttcc aaatcctacg
     4201 attgtcacac ggtgctgcca aaagcagact cattgcgcct ggccaacgat tccatcattg
     4261 aataacccgg atggtcaggc atatattcca atcattataa atccaatcca tctctcatgc
     4321 aaattgaaaa aaacactgta gtctcactgt gctatacctt gaaagccgac aattcagaag
     4381 gagatatcat tgaagtagcc caggaaactg atccgctcgt atttttatat ggtgccggtc
     4441 agatgttgcc aaagtttgaa gaaaacctgg caaacctcaa acaaggcgat cctttcgaat
     4501 tcgggctgct aagtaccgat gcatacggcg atcatgatcc cgaagctgtc atcgacctgg
     4561 atattgaaat ttttaaagtt gacggcaaac tggatgaaga aatggttgct cccggcaata
     4621 tgatcccgat gcgcgactcg gaaggccata tgttacaggg taaagtggtc agcatttcac
     4681 cagtcgctgt taaaatggat tttaatcatc ccatggccgg tcaaaacctg catttctcag
     4741 gctcaatcct tgaagtaagg caggcaacag aagaagaact gaaccacggg catgttcatg
     4801 gctgttgcgg gcacgaccat taatcccggt cgttaaaaat aaaaatgagg ttgtcgcaac
     4861 aaggcaacct ctttttttta tcacccaaaa caacggatgt gaaaatgaaa tgtccgtatt
     4921 atctaaggac aaaagggaca acgatggcaa taagtcctgt tcctgccaca accgaaatga
     4981 agaggttgta acgtacactg taattgttca gcagggattc atcataatac ttgtcaacat
     5041 ccttatcctt ataaatcttc ataagcccgg aaatgagttt cctgcgtgtc tgcttgcctt
     5101 tcagcaaacc tatcaccact accaggttta ccagcaccag aagcactaca aacaggaaca
     5161 tggctacaaa cgtggattca ttggttcttg atttttcagc catcccccaa ttcacggcaa
     5221 gggtgataag gttgagcgct attgaagtaa gtataaatat agtatcagtc cgtgcgtttt
     5281 gctgcaattc agaggtgatg tgattgtgga catattctat cataacaatg cttcttgaag
     5341 attccagtcc ggtttatctg aatttccaaa ctttggcctt tttataaggc catccttcca
     5401 ggaactgata tctgccgtgg ggaaatatgg cacttatggc aacagccgtt ccgatgccgg
     5461 caacaatccc gatggtactt cccagcgccc agttcctgta actttccgga ttgatttttc
     5521 cgtctgaaaa gttatagcag atcaacggag agaaaatcaa aaccagcagc gaccccaaaa
     5581 tgaccggttc gccgatttcg ctcatacctc tccatttttc aaagctgtaa tccaacgaat
     5641 gaatatcgga caaggcaatg cgcatcatat tggtatcggc tgacggggtt gagaggtagt
     5701 attgttttgc aggaacgacg ctggtgtaat ggatgccgtt tgtgtataac tggtgcgccg
     5761 taatggtttt tagcttcatc tgcagcgaat cggccgatcc gcttataaat tcaccatagt
     5821 aatatttgta ctgtttcagg gtatcggtaa ccggcagttg gtttccgacg gtcagttgca
     5881 tggttggctt gatccttagg gtttttaccc tgtggctgcc ggtggttttt tctacccgga
     5941 ctttccatgt ttgggcgttc aggatcatgg ataacgcggc cagtattaag gataaaagta
     6001 tggttctcat agctgctggt taaagtcaca tttgcgcatt caggtcaggt taacaggtaa
     6061 tttattcagg tttccggttt aacacttccc ccttgataac aaacaatacc cctgcaatta
     6121 aaatcggcgc tatcagaatc agggcggccg cattcccggt gaaaacctta aagagaatac
     6181 ttccggcaaa tgccgccaca atgaacagga tgccgccggc aagttcccat ttccaggcaa
     6241 tgataatcag tatgatgagt gtgaaagtcg gaatgttatg catcacgaaa cagatgagtt
     6301 gctctttcag ggaatagtct ccacctatgc aatcaaagga cattattaat atgaagagta
     6361 tagacaggag ggaaagaatc cgggcagacc aattgacaat tttgctgttc ataagtataa
     6421 ttttatttaa aagggctgac aattctgtag ttccggaata tttttgaccc gtttctgagg
     6481 tttgccggct tgaatctgat gtttacagga gcttccggcg cttctgtact cagtttacta
     6541 tcctaagccg gcggctggtt ctgggatttt tcttttgcca gctcttttgt cagggctgtg
     6601 agtccggcaa cacttcccaa ctgcattgtt tcaacggctg tactgggcac cacgataatg
     6661 gtagcattgg tcttgagccc ttcgtacaac atgttcatgg cgcgaaggtg aaaagcggtt
     6721 ggattgtcgg catacgtttg tgcggcttct tcaaactttt gggcaacctg cctttccgaa
     6781 tcgccaagga taacacgcgc ctggcgttcg cgttcagcct gggcctgcat cgacatggca
     6841 tcttcgaggc cggatggaat aaggacatct ttcacttcaa ccgaaataac cgtgataccc
     6901 cagggctctg tccgttcgtc gatgattttt tgaagcagga tgctcatttt atcgcgtcct
     6961 tcaagcatat ccgataacat tgttttcccg atgacatccc tgagagcagt ttgggatgcc
     7021 caactgatag cgctctggta ctcggcaaca tcaagcgccg cttttttagg atcgatcact
     7081 ttccagaaaa gcacggcatc cacatctacc gggactgtgt cgcgggtaag cgttctttcg
     7141 gctttaaagg tagttgtgat gaccctgata tcgatccagt aaggcacagc atcaattacc
     7201 gggatgatga aaaacaaccc cgggccacgc agcctgtgga attttccaag cctcagaacg
     7261 atggccctgt tccactgatc ggctactttg gtggcagagg ctgcgatgag cgccagcagg
     7321 aacgatccaa tcccgacggc tgtggtggta ccggaggtcg ccgtatcctt catggaatag
     7381 gccaatacga tcccggcagc aaggatgaca atgaacaata gtgcagaaaa aaaactgttg
     7441 tttttcatgg ctatgatcta tttatcgtga atcataatat ccggggaaac ctgttcactg
     7501 ttttcgatcg cattccaagc ttgtgatttc gggagctgca ttccgggtaa aggattccgt
     7561 ttccggattt catttagtac attataaata tacgacatta ttaccctgaa accaaattcg
     7621 ccggaaccgg tcaaagatgt tgatctccat ccgatactga cgcctgttct cgttttctga
     7681 gaatgctgta ttccaaagga atgcgaatgt gtccgcagtt aaacttcgct gaattaagtg
     7741 ccggttgttt tctaaaagct gctgatagct ttcagggtaa ggagatatga aaattgaaaa
     7801 aatggatagt atagtgaaga atagttgtgg ctttcgtcct ttggaaaagg gcagggaaaa
     7861 ccgttattgc attaaaaaaa ggctgtttca gaagccctta tatacttctg aaacagcctc
     7921 cggctgatag agtgactatt tcgatttctt gtctaaaatc ttttcaattt catccatgac
     7981 tttattcatc gactggatcg tcttttcaaa tggctcggac gttgtcatgt caacgcctgc
     8041 ttttttgagc aggtctatcg ggtagtccga accgccggac gaaaggaatt cgatgtattt
     8101 gttcagcgca ttcttatcgc cgctcatcac tttttcggca agcgatatgg atgccgtaaa
     8161 tgaagtgctg tactgataaa catagaaatt gtaatagaaa tgagggatat aggcccattc
     8221 cattgcgata taatcgtcga ttttgcagat tcccttatcg tggccatagt atgcttttac
     8281 aatgtcggca tacatcttcg aaaacgtttc gcctgtcaaa ggttctcctc tttcggctgc
     8341 ttcgtgtatt tttagttcga attctgcaaa ctgcgtctga cggaacaagg tgcctttaaa
     8401 tccgtcgagc cagttcatga gcaacgaaag cttgacgtcg tcgttttttt cgttcttcag
     8461 catgtagtta aacagcaaca cttcgttgaa agtagaagct acttcggcta caaatgtctg
     8521 gtaatcggca agcgggaaag gctgcgtttt gttcgaataa tagctttgca tggtgtgtcc
     8581 cagttcatgg gccatggtgc tcacatcatc atacaggccg ttataattca gtttgatgaa
     8641 aggatgcccg tcatagtaag aaccgtcgga ataggcgccg gtttgttttc cctgggtagg
     8701 gtatacatcg atccagcgct catcaatggc ctttttcacc gtggaaacgt attcttcccc
     8761 catcggtttt aatgcctcga gaatgatttt cgaggcttct tcatagctgt atttcaggtc
     8821 aacatctttt accaccggag cataaaggtc catatatttc aaggtgtcga gattcatcat
     8881 tcgttttttg atctgcaggt accggtgaaa agccggtagg cttttatcaa cattactgat
     8941 taatgaccgg taaacctcga cgggtatgtt ggagggatat aaggatgctt cgagcgatga
     9001 ctgatagttg cgggctttgg cctggaaaat atgcgactgt acatttccgt aaagcatctc
     9061 gccatatgag gctttaaact tatcgtagtt gtcccagaag gctttgaaca ctatctgccg
     9121 gtcttcgcgg tttgccgaag cacgataccg gctgtattcc ggtttggtaa ccgtgatctt
     9181 tgtaccgtcc gaaagagtcg cttcgggcga gggcatttcg gcatttgaaa aggtattata
     9241 gactgattcc ggaacgccgc cgaccggccc cgaaagggcg agaatacgct cttcggcttc
     9301 gctcagcgta tgctttttca tgcggaacat attatccagt tccatgcggt aaggcgcaag
     9361 acggggttcc gatttgatga acccgtcaat cagactccag tcggccgaca gtatctcagg
     9421 ctcgataaag gctgattttg cagcgaagcc ggaaaatatt tgctgcagtt cctgtttcat
     9481 ccccgaatat ttcatgtcgc gggtgtccag gtcggagttt aagttggcat acaggtataa
     9541 tttattggct tctttcctga tcccggagtt gtattccata cacctgagca aatcggaagc
     9601 agacctggtc aaagttcctc tgaattttac aacttcatcc atcttcttca caatgctttc
     9661 cttcgatgca cgccaatctg catcggattt gtaaatgtcg gtgagattcc atttgtagtt
     9721 gtcgggaatc tcggttcttg tcttctgact gtaactgttc atggccataa acgtactaaa
     9781 aacaactgca aacgctgttt ttactgttaa tctgtatcgg ttcattatta ctttaagtat
     9841 ggggttatga tggtgcaaga ttaccaaaaa ttcaccagaa agcggctatc ggatccttcc
     9901 ggcataaaat tttcccggcc ttttgcccgt gatccggaat acaggattct ttatttccgg
     9961 gaaaccggct tatgacaact cctggaacag cgtaatgatt gaccggccca gcctcatttt
    10021 taaccctgcc gtctcaggca aaagagaaca attgtttatt tataacacgc tgataattac
    10081 atggattggt aaatttaccg gaattattcc gtatatttga tatacaatct catagattat
    10141 cactatttat tcctgacagg cttgtgtttc ggaaaccttt gtatggtcct gattgagatg
    10201 gctgtacaac aggccctgca tcatcacttc tcttaaacaa cctcagttta ggttcccaaa
    10261 cttgtctcaa ttttaccggg gaagaattca cagagatacg tctttgcagt tactcatttt
    10321 catctcaacg aaagggggta ttaggaacag gaaacagcag atcagtcagt atcgccttaa
    10381 acatttacct gctaaacccg gtcctgatcg gactggttga gaaattattt tgtaaccatg
    10441 aaccattaaa agcgaaaacc aatgaaaaac attttacgat tgttcagatg gcttcccgtt
    10501 ctttttctgc tccttgtgag cattaacagg ctgacggcac aggttaccta tgatatctat
    10561 gtttacagcc tcatagacgg gacaacagtt caggcttccg taacccccgg ttattacgaa
    10621 tacaatcgaa gctggtctcc caacggtaaa aaactggccc atgaagaatg cggcttcgac
    10681 atcaacggga actggtacca gaatatttat atcactgaga tcagcagcca tgaaaccact
    10741 ccgcttgccg gcggcgaaaa cgggaacgat gcggcctggt cacctgaagg gaaaaagatc
    10801 atgtttgata aagtgaccga cgaatgggga tattcgggca ttttttgcgt tcctgacgaa
    10861 ggcggggacc cgacattgct gaggaattac ggaatcatgg cggagtggtc gcctaacagt
    10921 cattatatcg tattcctgga cgaagataca tggtggctta aaaccatgca cctggatgac
    10981 ggtgatgaga cgtttgtggt tttcggggcc gagaatcccg actggtcgcc caacggccaa
    11041 tacatcgttt acgacgaatg gtggtgctgt ggcggaatct ggatcataga ggttgatcac
    11101 gccggaaatc ctgtcggcga tcccgttcaa ttgacttcca ccggcggcca gcctacctgg
    11161 tcgaacaaca gcaagactat ttactatcat gatgcgtctc ctgacgggca atacgatatt
    11221 tattcaatat ccgttgaagg gggaactccg gaactggtat gcggtttgga tattccggat
    11281 tacggcaatt atgacccctg cgcttcgaat aacggaaaat atattgcttt cgccggggct
    11341 accgttcctg gcgacaaaag cgggccggcc ggttttcagc atgatgctga acaggaaagt
    11401 ggcctgttgg taactgtaat agggaatccc ggcgcagaag ggttcaaact gaatatcaga
    11461 agcaattctc cggaatcaat ttcattgcgc atcatcgatt cggaaggccg cgttgtatcc
    11521 gatcaaaccg gaattcagcc taacagcatg ctgggcatcg gaaacggcta taccccgggc
    11581 atttatctgg ccgaaatccg tcagggtgca caatttaaag tgatcaggct gatgaaacaa
    11641 taatccgcag ggttcgaaat ctgtaattct ctgatcggaa gaaaacacag tctgaaattg
    11701 tatcattcga tcatgtttcc tgttttaaat gtaacctaaa acccatttca atgaaaaaag
    11761 cgattatctc aacattcgcg gctgtcatca tgttgtcagt ctttacaaca gtatcagcgc
    11821 aggaatggac aaaagcccag aaggaagtct ggcaggctgt tgataacatc tggtcgaatc
    11881 tgaaagccgg aaatcttgac ggatttgcag ccggttttca cgaaaaatac cagggatgga
    11941 ataattacct gcctttgccg gtcggtaaac aaaaaaatat tgcgtcgtac agggaaaata
    12001 aagatatcca gaaactggaa agttatgaaa tagaaccggc ccggattacc gtaagcgaga
    12061 attccgccgt agtcgactac tacttttcga taacgatttc cgaaaagaaa gacgggaaga
    12121 gtgaggttaa ggtttactcg ggcaaaaatg ttgaattttt tgtgaaagaa gagggaaaat
    12181 ggttgttgct cggcgatatg acatatataa agacggatgg aaagtagctg cagccgcccg
    12241 acggtcggaa aaaatcccgg gaaggtttca tcccgggatt ttttatgcat ttaaaattga
    12301 aagtgaatga cttacatacc ttcgtaataa ctttatctgt ttccggctat ctgccagata
    12361 aagacggatc tcaacacaac atcgaagttg ccaaattaag cgggaagttt agcctaaggc
    12421 cggctttgtt gataattggc ctgccggcca catcatcgcc atggtatcac cggatcattc
    12481 ctttttttct ctcacagcta acccccaacc gatggcggct ccggtaattc cgtatatgac
    12541 gacggttgca agcgcatcaa cgaggatggc tttcagattg tacaggttcc acatcgacat
    12601 caacgagaaa tcatacatca cggcaatgaa aaacccgatg atcagtccac cgatcaatcc
    12661 gcccttcagg gtggaaatcc cggcccacct tttaaagata aaagccagga aatacccggt
    12721 tgccaaacat gaaatgacta acatccacaa caccggcatt tctttggtaa ggccttcata
    12781 atgaattgta tttgcattca ggaatggcat cagcagcttt ccgtaaacca gccagccaag
    12841 gaaaaagtta ataattcccc cgaagaggcc cgacgcgata aactttgctt tcatggtttt
    12901 ggttttagtt taacatccga taaagttacg gagtactaag caaaaacctg ctttttctgg
    12961 tggcgaaatc ttaaagaatg cggttgttgt tgcattaagc tggcctgtca ggttccgtta
    13021 tttcccattt ttcaaccttg ccttcaacca cgacaaataa tggatgaggc tcaatatgag
    13081 tgagtttgat gaattggcga tgccgctccg ggtcccggat ttttaattta ttaagcaggt
    13141 gatgatattc atattccatt tgtccgcttg taaccgaaag gcgataatcc ccgtcgccgg
    13201 catcaaattt tgagctgtcg aaatggtatc ctctttcaac cgcttcctga taaacggatt
    13261 gaagataacg gctgatcatt ccgggcggat tttccgtctg cctgaatctt tccagttggg
    13321 gatggtgacg gtaccctttc gttttgccct caagcacctt ttttgccagc aacgtctctc
    13381 tccagagggc gatgagccct ttgccgtcga gatacttcgg atgtaacgac cagattctca
    13441 tttttttatc atttctgaaa cccgaaaaac cgattattat ctaatttagt attaaacaga
    13501 ttgacagaat ctttgtttat caacttgagc tcaggaatga cctgctcaaa accggtattt
    13561 tgagtcagca ccagccctct cccttatttg tgccatttgg caatttgtag taagatgtga
    13621 agttatgaat cagaaaaaga gtttttcggc tttagccctg gcaggtttag ccctgctcac
    13681 ccagtcctgt gcctccatcc cgaaaggagc gaccgttgtc aaaccttttg ataaagaaag
    13741 atacctgggt aaatggtatg aaataggacg gctggaccac cgtttcgaac ggaacctgga
    13801 caacgttacg gctgtttatt cgatgaataa tgacggtacg attaaagtga caaacagcgg
    13861 atataaaaca acgacgaaag aatggaaaca ggttaccggc aaagctaaac cgtcgggaaa
    13921 gcccggtgag ggaaagctga aagtgtcgtt cttcgggcct ttttacggcg cttacaacat
    13981 cattgcgctc gatgagcaat acagatacgc cctggtggcc ggtaacaacc tgaaatactt
    14041 gtggatattg tcccgtgaaa cgtccatccc cgaagatatc aaactgcaat tccttgcgac
    14101 agcaaaatct gtggggtatg atacctcgtc gctgatttgg gtgaaacatg ataacggcaa
    14161 atagaattct ggcctgattg aacaatcttt cgtgtaattc gtggaaatgt tttctccacg
    14221 aatggacacg aatgaagcac gaatgcacac gaactgatta ttcagaatcc agggaacatt
    14281 tcgtgataat tcgtgtcccg gcgcagcctg tttcgtgtaa ttcgtggtta agttttctcc
    14341 acgaatggac acgaatgaag cacgaatgca cacgaactga ttattcagaa tccagggaac
    14401 atttcgtgat aattcgtgtc ccggcgcagc ctgtttcgtg taattcgtgg ttaa
//
LOCUS       DAOPYV010000071        14374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_4586_length_14374_cov_11.411691, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000071 DAOPYV010000000
VERSION     DAOPYV010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14374
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4586_length_14374_cov_11.411691"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /gene="rplB"
                     /locus_tag="PKI35_09315"
     CDS             <1..698
                     /gene="rplB"
                     /locus_tag="PKI35_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNX44621.1"
                     /translation="NNEGKMTMRYMGGGHKQQYRIIDFKREKENIPAVVKTIEYDPNR
                     TARIALLHYADGEKRYIVAPHGLKVGQEVLSGKGVAPEIGNTLYLSEIPFGTLIHNVE
                     LRPGQGAKMVRSAGASAQLMSRDGKFAVLKMPSGETRLILQACKATIGMVSNGDHSLE
                     SSGKAGRSRWLGRRPRVRGVVMNPVDHPMGGGEGRASGGHPRTRKGTPAKGYKTRSKT
                     KDSNKYIIERRKK"
     gene            715..993
                     /gene="rpsS"
                     /locus_tag="PKI35_09320"
     CDS             715..993
                     /gene="rpsS"
                     /locus_tag="PKI35_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNX44622.1"
                     /translation="MSRSLKKGPFVDYKLEKKVLEHVDSKKKVVIKTWSRASTITPDF
                     VGQTIAVHNGNKFIPVYVTENMVGHKLGEFAPTRIFRGHAGNKDKGKK"
     gene            1008..1439
                     /gene="rplV"
                     /locus_tag="PKI35_09325"
     CDS             1008..1439
                     /gene="rplV"
                     /locus_tag="PKI35_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNX44623.1"
                     /translation="MGARKHNTAELKKEANKQLYFAKLNNCPSSPRKMRLVADLIRGK
                     NVELALNILQHSPNYAAEKLFKVLKSAIANWQQKNEGVRMEDSHLFVSEVQVDGGRIL
                     KRIQPAPQGRAHRVRKRSNHVTVFVDSRNAKVETETEIVTK"
     gene            1452..2198
                     /gene="rpsC"
                     /locus_tag="PKI35_09330"
     CDS             1452..2198
                     /gene="rpsC"
                     /locus_tag="PKI35_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNX44624.1"
                     /translation="MGQKTNPIGNRLGIIKGWDSNWYGGRKFENKLVEDDKIRKYLNA
                     RLSKAGISRIIIERTLKLVTVTINTARPGIIIGKAGQEVDKLKEELKKITGKEVQINI
                     SEIKRPEIDAVIVANTIARQIEGRISYRRAIKTSIASAMRIGAEGIKVFISGRLGGAE
                     MARREQFKDGRIPLHTLRADIDYALAEAHTTYGRIGIKVWICKGEVYGKVDLVPSNEG
                     VIKDKGTPAPGGRPGKGPRHGGDRRPRNKK"
     gene            2241..2669
                     /gene="rplP"
                     /locus_tag="PKI35_09335"
     CDS             2241..2669
                     /gene="rplP"
                     /locus_tag="PKI35_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNX44625.1"
                     /translation="MLQPKKTKYRKQQKGKMKGNTKRGAELSFGSFGIKSLEECWMTG
                     RQIEAARQAITRHMKREGQLWIRIFPDKPVTKKPAEVRMGKGKGAPEYFVARVSPGCM
                     LFEVEGVSEEIAKESLRLGAQKLPIATKYVKRRDYDPELA"
     gene            2719..2940
                     /gene="rpmC"
                     /locus_tag="PKI35_09340"
     CDS             2719..2940
                     /gene="rpmC"
                     /locus_tag="PKI35_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNX44626.1"
                     /translation="MKQQVIRELSNPELKERLDEERVQLTKLKMNHAVSPLENPNKIK
                     AYRKTVARLLTEVHSRELESAKAKEAKNK"
     gene            2940..3209
                     /gene="rpsQ"
                     /locus_tag="PKI35_09345"
     CDS             2940..3209
                     /gene="rpsQ"
                     /locus_tag="PKI35_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNX44627.1"
                     /translation="MFTAMEERNLRKEKIGIVVSNKMQKSIVVEVERRVKHPKYGKFV
                     KKTSRFAAHDEKDECGIGDTVRIAETRPLSKTKCWRLVEIIERAK"
     gene            3212..3580
                     /gene="rplN"
                     /locus_tag="PKI35_09350"
     CDS             3212..3580
                     /gene="rplN"
                     /locus_tag="PKI35_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNX44628.1"
                     /translation="MIQQESRLTVADNSGAKEVLCIRVLGGTRRRYASVGDKIIVSVK
                     NALPSGNIKKGVVSKAVVVRTKKEIRRVDGSYIRFDDNAVVLLNNTGEMIGTRIFGPV
                     ARELREKQYMKIVSLAPEVL"
     gene            3593..3913
                     /gene="rplX"
                     /locus_tag="PKI35_09355"
     CDS             3593..3913
                     /gene="rplX"
                     /locus_tag="PKI35_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNX44629.1"
                     /translation="MSKLHIRKGDNVKVLAGDSKGKTGKVLEVDVEKYKAIVENVNLA
                     TKHTKPNAKNPKGGILKQEMPIHLSNLMVLDAGGKPTRTGHQSDTKTGNSVRVSNKSG
                     EVIK"
     gene            3913..4461
                     /gene="rplE"
                     /locus_tag="PKI35_09360"
     CDS             3913..4461
                     /gene="rplE"
                     /locus_tag="PKI35_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNX44630.1"
                     /translation="MNYTPRLRDKYFAEIVPALKEQFQYKNNMQVPKLAKICINQGLG
                     TAIADKKIIDVGLAEMSSIAGQKAVSTKSRKDISNFKLRRGMPIGVRVTLRGEKMYEF
                     LDRLVSVAIPRIRDFRGINEKGFDGRGNYTMGITEQIIFPEIVIDKVMKINGLDITFV
                     TTARTDKECLALLKEFGLPFKK"
     gene            4472..4741
                     /gene="rpsN"
                     /locus_tag="PKI35_09365"
     CDS             4472..4741
                     /gene="rpsN"
                     /locus_tag="PKI35_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNX44631.1"
                     /translation="MAKESIKAREVKRQKMVAKYAAKRAALKAAGDYTALQKLPRNAS
                     PVRLHNRCKITGRPKGYMRQFGISRINFRFMALEGLIPGVRKASW"
     gene            4779..5177
                     /gene="rpsH"
                     /locus_tag="PKI35_09370"
     CDS             4779..5177
                     /gene="rpsH"
                     /locus_tag="PKI35_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNX44632.1"
                     /translation="MFTDPIADYLTRIRNAVMANHRVVEIPASNLKKEITKILFEQGY
                     ILNYKFEEETHPGIIKIALKYHPVTKQSAITQIERVSKPGLRRYVGAEKLPRVLNGLG
                     TAIVSTSKGLMTEKEARKQGIGGEVICYVS"
     gene            5195..5752
                     /gene="rplF"
                     /locus_tag="PKI35_09375"
     CDS             5195..5752
                     /gene="rplF"
                     /locus_tag="PKI35_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNX44633.1"
                     /translation="MSRIGKLPIAVPSGVQITVSDKNLVTVKGNLGELKQQVDPDITV
                     KVENGHLLVERPTDQKRHRAMHGLYRSLLANMVKGVSEGYKIEQELVGVGYKAVATGQ
                     VLELSLGYSHNIIFEMPAEIKVTTVTERGKSPLITLVSHDKQLIGQVAAKLRSFRKPE
                     PYKGKGVKFVGEVLKRKAGKSASDK"
     gene            5782..6144
                     /gene="rplR"
                     /locus_tag="PKI35_09380"
     CDS             5782..6144
                     /gene="rplR"
                     /locus_tag="PKI35_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNX44634.1"
                     /translation="MSFKVDKEYRRLKIKQRVRKVISGTTERPRLTVFRSNKEIYAQV
                     IDDLAGKTLVSASTRVKGIATQKGTKTEKAQLVGKLVAERALEAGINTVVFDRNGYLY
                     HGRIKALAEAAREGGLKF"
     gene            6153..6671
                     /gene="rpsE"
                     /locus_tag="PKI35_09385"
     CDS             6153..6671
                     /gene="rpsE"
                     /locus_tag="PKI35_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNX44635.1"
                     /translation="MANANIKRVKSSEIEFKDRLVAIQRVTKVTKGGRTFSFSAIVVV
                     GNENGVVGYGLGKAKEVQAAISKGIDDAKKNLIKVPVLKATIPHEQYGKYGGALVFLK
                     PAAPGTGVIAGGAMRAVLESVGVKDVLAKSKGSSNPHSVVKATIDALAQMKDPYTIAQ
                     LRGISLTKVFNG"
     gene            6691..6870
                     /gene="rpmD"
                     /locus_tag="PKI35_09390"
     CDS             6691..6870
                     /gene="rpmD"
                     /locus_tag="PKI35_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNX44636.1"
                     /translation="MKKVRITQIKSGIKQPERQKRTLKALGISKMNRPVEVEATPQIE
                     GMIKAISHLLKVEEI"
     gene            6899..7345
                     /gene="rplO"
                     /locus_tag="PKI35_09395"
     CDS             6899..7345
                     /gene="rplO"
                     /locus_tag="PKI35_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNX44637.1"
                     /translation="MDLSNLKPAEGSVKTSKRVGRGQGSGRGGTSTRGHKGAKSRSGY
                     SRKIGHEGGQMPLYRRVPKSGFKNINRIEYSGINIDVLQKLAESKGLTAIDPETLRQN
                     GLLGKSDLLKVLGRGELKQKLDVTAHAFSAKAIQAIETAGGTATKL"
     gene            7348..8709
                     /gene="secY"
                     /locus_tag="PKI35_09400"
     CDS             7348..8709
                     /gene="secY"
                     /locus_tag="PKI35_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNX44638.1"
                     /translation="MKRLIQTLRNIYKIEELRKRILYTLGIIVLYRLGSYVVLPGIDP
                     NQLATLQNQGKEGLLGLLNMFSGGAFSNASIFALGIMPYISASIVVQLLGMAIPYFQK
                     LQKEGESGRKKINQITRYLTVVIVALQAPAYITNLISQLPPQAITPFDPNITSPNTFF
                     WISSVIILISGTMFVMWLGEKITDKGIGNGISLIIMIGIIARLPFALFGEFISRMEQK
                     GGGLVVFLVELVVLVVVILLCILLVQGTRKIPVQYAKRIVGNKQYGGVRQYIPLKVNA
                     AGVMPIIFAQAIMFLPLTIAGFANSESLRGFAAAFTDINGFWYNLVYALLIIAFTYFY
                     TAITVNPNQMAEDMKKNGGFIPGVKPGKKTADFIDSVMSRITLPGSIFLAFVAVMPAL
                     VRMTGVNSQFAQFYGGTSLLILVGVVLDTLQQIESHLLMRHYEGLTKSGRIKGRSSYA
                     SAY"
     gene            8730..9521
                     /gene="map"
                     /locus_tag="PKI35_09405"
     CDS             8730..9521
                     /gene="map"
                     /locus_tag="PKI35_09405"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNX44639.1"
                     /translation="MALVKTDIEIEKVRKSSLLVGETIAEVARHLRAGITTSELDRIA
                     YEFILDHGALPAFKDYNGYPATLCISVNDVVVHGIPGNQELKEGDLVSIDCGTVIDGY
                     YGDSAFSFGIGEMDPEVENLMKRTKESLYKGIDVAVAGKRIGDIGYEVQSYVEDFGYS
                     VVRDLVGHGLGNHLHERPEVPNYGKRGTGPKLEENMVICIEPMINLGKRVVMQDRDGW
                     TIRTIDHKPSAHFEHAVAIRKDKADILSSFETIEQVVNHNILIIK"
     gene            9524..9742
                     /gene="infA"
                     /locus_tag="PKI35_09410"
     CDS             9524..9742
                     /gene="infA"
                     /locus_tag="PKI35_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNX44640.1"
                     /translation="MAKQLVIEQDGVVRESLGNAMFRVELENGHVITAHISGKMRMHY
                     IKILPGDKVKIEMSPYDLTKGRIIFRYK"
     gene            9781..9897
                     /gene="ykgO"
                     /locus_tag="PKI35_09415"
     CDS             9781..9897
                     /gene="ykgO"
                     /locus_tag="PKI35_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HNX44641.1"
                     /translation="MKVRASVKKRTPDCKIVRRKGRIYVINKKNPKFKQRQG"
     gene            9929..10309
                     /gene="rpsM"
                     /locus_tag="PKI35_09420"
     CDS             9929..10309
                     /gene="rpsM"
                     /locus_tag="PKI35_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNX44642.1"
                     /translation="MARIAGIDLPKNKRGEIGLTYIYGIGRSTAQHILDKAGIDRGKK
                     VQDWNDDEQNQIRSIIGEEIKVEGALRSEVQLNIKRLMDIGCYRGIRHRLGLPVRGQS
                     TKNNARTRKGRKKTVANKKKATKG"
     gene            10343..10732
                     /gene="rpsK"
                     /locus_tag="PKI35_09425"
     CDS             10343..10732
                     /gene="rpsK"
                     /locus_tag="PKI35_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNX44643.1"
                     /translation="MAKTTKVSKKKVVKVDAVGRAYVFASFNNIIISLTNSTGQVISW
                     ASAGKMGFRGSKKNTPYAAQMAATDCAKVAYDLGLRKVKVYVKGPGSGRESAIRTLHT
                     AGIEVTEIQDVTPLPHNGCRPPKRRRV"
     gene            10792..11397
                     /gene="rpsD"
                     /locus_tag="PKI35_09430"
     CDS             10792..11397
                     /gene="rpsD"
                     /locus_tag="PKI35_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNX44644.1"
                     /translation="MARYIGPKSKIARKFKEPIYGPDKAYEKKNYPPGQHGQNKRRRK
                     VSEYGTQLAEKQKAKYTYGILEKQFKNTFLKATSKKGITGEILLQLIEARLDNVVYRL
                     GIAPTRDAARQLVSHRHITVNGKVVNVPSYELLQGDVVAVRERSKSLEVIQNNMTGRG
                     SRFSWLEWDEASLTGKFINYPQRDEIPENIQEQLIVELYSK"
     gene            11437..12429
                     /locus_tag="PKI35_09435"
     CDS             11437..12429
                     /locus_tag="PKI35_09435"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNX44645.1"
                     /translation="MAILSFQKPDKVIMVQADEFDGTFEFRPLEPGFGITIGNALRRI
                     LLSSLEGYAITSVRIDGVDHEFSSIKGVVEDVTDVVLNLKKLRFRKQIEAEESEKVNF
                     SFTGKDIFTAGDIAKHLSVFQVLNPDVVLCHMEPSVKLNIELTINKGRGYVPAEENKV
                     PNALIGTIFIDSIHTPIKNVKYTIENYRVEQKTDYEKLVLDIKTDGSIHPKEALKEAA
                     KILIHHFMLFSDEKITIDTEEKAVTEEFDESTLHIRQLLKTKLVDMDLSVRALNCLKA
                     ADVETLGDLVAFNKNDLLKFRNFGKKSLTELEDLVKTKGLEFGMNIAKYKLDKE"
     gene            12435..13025
                     /gene="rplQ"
                     /locus_tag="PKI35_09440"
     CDS             12435..13025
                     /gene="rplQ"
                     /locus_tag="PKI35_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNX44646.1"
                     /translation="MRHQKAINHLGRTSTHRKAMLSNMASSLIRHKRINTTLAKAKAL
                     RTFIEPILTKAKDDSTHSRRIAFSYLQDKEAVTELFRTVAVKIADRPGGYTRILKTGF
                     RLGDNAEMCMMELVDFNEVMLAAKEAAPKKTTRRRRGTGKKAESTAEAVAEPTAKAAP
                     KAKAKAKAAPVAETEKPEEPVVDTEKTESDTPEDNQ"
     gene            13113..>14374
                     /gene="eno"
                     /locus_tag="PKI35_09445"
     CDS             13113..>14374
                     /gene="eno"
                     /locus_tag="PKI35_09445"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNX44647.1"
                     /translation="MSQIANITARQILDSRGNPTIEVDVFTTNGIMGRAAVPSGASTG
                     VHEAVELRDEDKSIYMGKGVLKAVQNVNKVLAEELKGYFVSEQNEIDARMIELDGTPN
                     KGNLGANAILGVSLAVANAAAQETGQYLYRYIGGVNANTLPIPMMNILNGGSHADNSI
                     DFQEFMIMPVGAPSFSEALRMGAEVFHNLKSVLKKAGYSTNVGDEGGFAPNLKSNEEA
                     ITVILQAIEKAGYKPGADVYIGLDPASSEYFIPEENVYHLHKSTGDKLTPAQMVDYWK
                     TWVEKYPIISIEDGMAEDDWDGWKLMTDVLGKKIQLVGDDLFVTNVKRLQMGIEKSVA
                     NSILIKVNQIGSLTETIDAVNLAYRNSYTAVMSHRSGETEDTTIADLSVALNTGLIKT
                     GSASRTDRIAKYNQLLRIEETLGSNARYL"
BASE COUNT         4463 a         3313 c         3389 g         3209 t
ORIGIN      
        1 ggaacaatga aggcaagatg accatgagat acatgggtgg cggccataaa cagcagtacc
       61 gtatcattga cttcaaacgt gaaaaggaaa acattcctgc agtggttaaa accattgaat
      121 acgatcctaa tcgtaccgcc cgtattgctt tacttcatta tgccgatggg gaaaaacgtt
      181 atatcgtagc tcctcacgga ctgaaagtgg gccaggaagt cctttcggga aaaggtgtag
      241 cccccgaaat cgggaacacc ctttacctga gtgaaatccc ttttggtacc cttatccata
      301 atgtggaact caggccggga cagggagcta aaatggtacg cagtgcaggg gcttcggcac
      361 aactgatgtc gcgcgacggt aaatttgccg tcctgaaaat gccctcgggc gaaacgcgcc
      421 tcattctcca ggcttgcaaa gctaccatag gcatggtttc aaacggagac cacagccttg
      481 aatcatcagg caaagcagga cgttcacgct ggcttggccg caggccccgc gttcgtggcg
      541 ttgtcatgaa tcctgttgac catcccatgg gcggtggtga aggacgtgct tccggaggcc
      601 atccgcgcac acgcaagggc acccctgcaa aaggttataa aacacgttcg aaaaccaaag
      661 attcgaacaa gtatattatt gaaagaagga agaagtaatt aaaaacatac tacaatgagc
      721 cgttcgctta aaaaaggccc cttcgtggat tataaacttg agaaaaaagt tctcgaacac
      781 gtcgacagca aaaagaaggt tgtcatcaaa acctggtcga gggcatcaac cataacaccc
      841 gacttcgttg gccaaaccat tgccgtacat aatggcaata aattcatacc ggtttatgta
      901 accgaaaata tggttggcca taaactaggc gaatttgccc ctacccgtat attcagggga
      961 catgccggaa acaaggataa aggtaagaag taaaaagaac tattgacatg ggagcaagaa
     1021 aacacaacac agcggaactt aagaaagaag cgaacaaaca gctctatttc gcaaagctga
     1081 ataattgccc ttcatcgccc cgcaaaatga ggctggtagc tgatcttatc aggggtaaaa
     1141 atgttgaact ggcattgaac attctgcagc attcgcctaa ctatgcagcc gaaaaacttt
     1201 tcaaggtgct gaaatcggcc atcgccaact ggcaacagaa aaacgaaggc gttcgtatgg
     1261 aagacagcca cctgtttgta agcgaagttc aggttgatgg cggccgcata ctgaagcgta
     1321 ttcagcctgc accccaggga cgtgcgcacc gtgtacgcaa gcgctcaaac catgttacgg
     1381 tgtttgttga cagcagaaat gctaaagttg aaactgaaac tgaaatagta actaaataat
     1441 taacaagcct aatgggacaa aagactaatc cgatcggcaa caggttaggt atcatcaaag
     1501 gatgggactc taattggtat ggaggaagga agttcgagaa caagctcgtt gaagacgata
     1561 aaatccgtaa atacctcaac gcacgtcttt caaaagcagg tatctcgcgc atcatcatcg
     1621 agcgcaccct taaactcgtc actgttacca ttaatacagc ccgcccggga attattatcg
     1681 gtaaagcagg tcaggaagtt gacaaactga aggaagagct gaagaagata accggcaagg
     1741 aagtccagat caacatttcc gaaataaaac gccctgaaat tgacgccgtt attgttgcca
     1801 ataccattgc aaggcagata gaaggacgta tttcataccg tcgggctatc aaaacatcca
     1861 ttgcttcggc aatgcggatc ggggccgaag gtatcaaggt gtttatttcc ggaaggttgg
     1921 gaggcgccga aatggcccgc cgcgaacaat ttaaagacgg acgtattcct ttgcataccc
     1981 tgcgtgccga catcgactac gctttggcag aagctcatac cacttacggc cgcatcggca
     2041 tcaaagtatg gatctgcaaa ggtgaagttt acggtaaagt cgatctggtt cccagcaacg
     2101 aaggcgttat taaagataaa ggaaccccgg caccgggagg caggccggga aaaggccccc
     2161 gtcatggcgg cgacaggcgt ccgcgcaata aaaagtaatc aattcagata aaggtttaat
     2221 cactatatac cgtaatagca atgttacagc cgaaaaaaac taaatacagg aagcagcaaa
     2281 agggcaagat gaaaggcaat accaaacgcg gtgctgaact ttcattcgga tccttcggta
     2341 taaaatccct ggaagaatgc tggatgacag gtcgccagat cgaagctgcc cgccaggcca
     2401 ttacccgtca catgaaacgt gaaggacagt tgtggattcg cattttcccc gacaagccgg
     2461 ttaccaaaaa gcctgccgaa gttcgtatgg gtaaaggtaa aggagctccg gagtattttg
     2521 ttgcccgtgt tagccccgga tgtatgcttt tcgaagttga aggcgtatcg gaagaaatag
     2581 caaaggaatc actcaggctt ggtgcccaga aactgcccat tgctaccaaa tacgtgaaac
     2641 gccgtgatta tgacccggaa ctggcataat ttccgaaaac gcaggtaaca ttattgacgc
     2701 aaaaacccaa aacgccatat gaaacagcaa gtaatccgtg aactgtcgaa ccccgagtta
     2761 aaagaaagac tcgatgagga acgcgttcaa cttaccaagc tcaaaatgaa ccatgcggtt
     2821 tctcctctcg agaaccccaa taaaataaag gcttatcgca aaactgttgc acggcttctt
     2881 accgaagtac acagccgcga attagagtca gcaaaggcaa aagaggctaa aaacaagtaa
     2941 ttttcacagc aatggaagaa agaaacctca ggaaagaaaa aatcggcatc gttgttagca
     3001 acaaaatgca gaaatccatc gtggttgaag tggaaagacg cgtaaagcat cccaaatacg
     3061 gaaaattcgt caagaaaaca tctcggttcg ccgcccacga cgaaaaagac gaatgcggaa
     3121 ttggggatac cgttcgtata gccgaaacca ggcctttgag caagaccaaa tgttggagat
     3181 tagttgaaat cattgaaaga gctaaataat tatgatacag caggaatcaa gacttacggt
     3241 tgccgataac agcggtgcaa aagaagtgct ctgcatcagg gtactcggtg gaacccgccg
     3301 gcgctatgcc tccgtaggcg acaagatcat cgtatcggtg aaaaacgccc tcccgtcagg
     3361 caatattaaa aagggcgttg tatcaaaagc agtggttgtc aggactaaaa aggaaatccg
     3421 ccgtgttgac ggttcgtata tccgtttcga tgacaatgcc gtcgtactgc tcaataacac
     3481 cggcgaaatg ataggaaccc gtattttcgg acccgttgcc agggaactga gggaaaaaca
     3541 gtatatgaaa attgtttcac tcgcaccaga agtgctttaa cactaagcta ttatgagcaa
     3601 actccatatc cgtaaaggcg ataatgtcaa ggttctggcc ggcgattcaa aagggaaaac
     3661 cggtaaggta ctcgaggtgg atgttgaaaa atacaaagcc atcgttgaaa atgtcaacct
     3721 ggctacaaaa cacaccaaac ccaacgccaa gaacccgaaa ggcggcatac tgaaacagga
     3781 aatgcctatt cacctttcga accttatggt ccttgatgcc ggtggcaaac caacacgcac
     3841 cggccatcaa tccgacacca agacaggtaa cagtgtacgt gtatccaata aatcagggga
     3901 ggtaattaaa taatgaacta tacaccacgc ctcagagata aatattttgc agagattgtc
     3961 ccggctctca aggaacagtt tcagtataag aacaatatgc aggtgccaaa actggcaaaa
     4021 atctgtatca accagggatt gggaaccgcc atcgccgata agaaaataat cgatgtcggt
     4081 ttggctgaaa tgtcctccat agcagggcag aaagctgtgt cgacaaaatc gcgcaaggat
     4141 atttcaaact tcaaactgcg tcgtggtatg ccgattggcg tgcgtgtaac gctgcgcggt
     4201 gaaaaaatgt atgaattcct tgaccggctc gtttcggttg ctattccccg tatccgcgac
     4261 ttccgcggga tcaacgaaaa aggttttgac ggccgtggca actataccat gggcatcacc
     4321 gagcagatca tattccccga aatcgtgatt gacaaggtaa tgaagatcaa cgggctggat
     4381 atcaccttcg ttaccactgc cagaactgat aaagagtgcc tggcattact gaaagaattc
     4441 ggtttaccat tcaagaaata accatataat catggcaaaa gaatccatta aggcacgtga
     4501 agtaaaaagg caaaaaatgg ttgccaaata tgctgccaaa agggctgccc ttaaagctgc
     4561 aggcgactat acagcactgc agaaattgcc cagaaatgca tcccccgttc gtttacacaa
     4621 ccgttgcaaa ataacaggga gacctaaagg ctatatgagg caattcggca tttcgcgcat
     4681 caatttccgt tttatggctc tcgaaggcct tatcccgggc gtgcgcaaag caagctggta
     4741 aaatattcaa acagaaataa caaatagaat agataacaat gttcacagat cccattgcag
     4801 attatctgac cagaataagg aatgccgtga tggccaacca cagggttgta gaaattcctg
     4861 catctaatct caagaaggag atcaccaaga tccttttcga acagggatac atcctcaact
     4921 ataaattcga ggaagaaacc caccccggaa taatcaagat cgccctcaag taccatccgg
     4981 taacaaaaca atcggccatt actcagattg aaagagtaag caaacccggt ttacgccgtt
     5041 atgttggcgc tgaaaagctc ccgcgcgtcc tcaacggatt aggcacagcc attgtgtcga
     5101 cgtcgaaagg actgatgacc gagaaagaag cccgcaaaca gggtatcggc ggagaagtaa
     5161 tttgttacgt tagttaataa aggaggaaag aagcatgtcg agaattggaa aattaccgat
     5221 tgcggttcca tcgggcgttc agattaccgt ttcggataaa aatcttgtta ccgttaaagg
     5281 caatcttgga gaactgaaac aacaggttga ccctgatatc accgtaaaag tagaaaacgg
     5341 acacttgctg gttgaacgtc caactgatca gaaaagacat cgtgccatgc atgggctgta
     5401 ccgttcgctc ttagccaata tggttaaagg tgtaagcgaa ggatataaaa ttgaacagga
     5461 attggtcggc gttggttaca aggctgtagc cacagggcag gtacttgaac tgtcgctcgg
     5521 gtattcgcac aacattattt ttgaaatgcc cgccgagatc aaagttacaa cggttaccga
     5581 acgtgggaaa agccctctga tcacccttgt atcgcacgat aaacaactta tcggtcaggt
     5641 agctgccaag ttgcggtcgt tccgtaagcc tgagccgtat aagggaaaag gtgtgaagtt
     5701 tgttggtgaa gtcctcaaac gtaaagccgg aaagtcggca tccgataaat aatcattgag
     5761 aacagcaaat acccataagc aatgtctttc aaggtagata aagaatatag aaggctcaag
     5821 atcaaacagc gcgtgcgcaa agtgatttcg ggaacaaccg aacgtccacg cctcaccgtg
     5881 ttccgcagca acaaggagat ttatgcccag gtaatcgacg acctggccgg gaaaaccctt
     5941 gtaagcgcct caacacgcgt caaaggaata gccacccaaa aaggtacaaa aaccgaaaaa
     6001 gcccaactgg ttggaaaact ggttgccgaa cgcgctctcg aagccggcat caatactgtg
     6061 gttttcgacc gcaacggata tttatatcat ggtagaatca aggcactggc tgaagcagct
     6121 cgcgaaggcg gccttaaatt ttaacaacga ctatggcaaa cgcaaatata aaaagagtaa
     6181 aatcgagcga aatcgaattt aaagacaggc tcgtcgccat ccagcgtgtt actaaagtca
     6241 ccaaaggggg ccgtacattc agtttctccg ccatcgtggt tgtgggaaat gaaaatggag
     6301 tcgtaggtta cggacttggc aaagccaagg aagttcaggc tgccatatcc aaaggtatag
     6361 atgatgcaaa gaaaaacctg atcaaggttc ctgtgctcaa agccactatc cctcacgaac
     6421 aatatggtaa atacggcgga gcccttgtct tcctgaaacc ggcagctccc ggtaccggtg
     6481 taatcgccgg tggcgctatg cgtgcagtgc tcgaaagtgt tggcgtaaaa gatgtgctgg
     6541 caaaatcaaa aggatcatca aacccccata gcgttgttaa ggctaccatt gatgcattgg
     6601 cccagatgaa agatccttat accattgccc agttacgtgg tatatcgctt accaaggtgt
     6661 ttaatggtta atccgctaaa agcaattgcg atgaaaaaag tcagaatcac acagataaag
     6721 agtggaatta aacaacccga gcgccagaaa cgtacgctta aagcactggg tatatccaag
     6781 atgaaccgcc cggtggaagt tgaagccaca ccgcagatcg aaggcatgat taaagccatc
     6841 agccacctgc tcaaagttga agaaatctaa ttgtaaacca tttacagaaa atattattat
     6901 ggatctcagc aatctcaaac cagccgaagg atcggtgaaa acatcgaaac gcgttggcag
     6961 aggacaaggt tccggccgcg gtggcacctc cacccgtggg cataaagggg cgaaatcccg
     7021 ttcgggatat agccgcaaga ttggacatga aggtggacaa atgcctttgt accgccgtgt
     7081 tcccaagtca ggttttaaaa atatcaaccg tattgaatac agcggcatca atatcgatgt
     7141 gcttcaaaaa ctggccgaaa gcaaaggcct taccgccatc gatcctgaaa cactgcgtca
     7201 aaacggcctt cttggtaaaa gcgatttgtt gaaagtgtta ggccgtggcg aattgaaaca
     7261 aaaactcgat gttacagccc atgctttttc agctaaagcc attcaagcca tcgaaactgc
     7321 tggcggaacc gcaacaaaac tctgacaatg aaacgactca tccaaacgct tcggaatatt
     7381 tacaagatag aagaactccg caaaaggata ctctatactc ttggcatcat tgtactgtac
     7441 aggcttggtt cgtatgtggt tttaccgggt attgacccga accaactggc tacattgcaa
     7501 aaccagggca aagaaggact tttaggactg ctcaacatgt tctccggcgg cgctttcagc
     7561 aacgcttcta tctttgcact tggtattatg ccgtatatct cggcctctat cgtggtacaa
     7621 ttgctgggta tggctatacc gtatttccag aaattgcaga aagaaggtga aagcggacgt
     7681 aagaaaatca accagataac ccgctacctt actgttgtga tcgttgctct gcaggctccg
     7741 gcttacatta ccaaccttat ttcacaactg cctccccagg ctatcacccc gtttgatccg
     7801 aatattacat cgcccaatac attcttctgg atctcatccg tcatcatcct gatttcaggt
     7861 accatgttcg tgatgtggtt aggtgaaaag ataacggata aagggatcgg taatggcatc
     7921 tcgctcatta tcatgatcgg tattattgcc cgcctgccgt tcgctttatt cggtgagttc
     7981 atttcacgga tggaacaaaa aggcggcgga cttgtcgttt tcctggttga actggttgtc
     8041 ctggttgtag tcattctgct ttgtatcctt ttggttcagg gaacacgtaa aattcccgta
     8101 caatacgcaa aacgcattgt gggcaacaaa caatatggcg gcgtaaggca gtatatcccc
     8161 ctcaaagtga atgctgccgg cgttatgcct atcatctttg cacaggctat catgttcctg
     8221 cctctgacca ttgccggttt tgcaaacagt gaatccttac ggggttttgc tgccgctttt
     8281 accgatatca acggtttctg gtataacctg gtgtatgccc tcctgatcat tgcctttact
     8341 tacttctata cagctatcac ggttaaccct aaccagatgg ctgaagatat gaaaaagaac
     8401 ggtggcttta ttcccggcgt gaaacccgga aagaaaaccg ctgacttcat tgattcggta
     8461 atgtcccgca ttaccctgcc gggttcaata ttccttgcct ttgtagctgt tatgcctgca
     8521 ttggtcagga tgaccggtgt caacagccag ttcgcccagt tttatggggg aacatccttg
     8581 ctgattctgg taggcgttgt gctggatacg ttgcagcaaa tagaaagtca cctgttgatg
     8641 cgtcattatg aaggcctcac caaatcaggc cgtatcaaag gacgttcatc ctatgcaagt
     8701 gcctattaat ctgtcggagc aggaaaataa tggccttggt aaaaactgac attgaaatag
     8761 aaaaagttcg caaaagttct ttgcttgttg gagaaacgat agcagaagtt gcccgtcacc
     8821 tcagggcagg tattactacg tcggaactgg acaggattgc ttatgagttc atcctggatc
     8881 acggagcttt gcctgctttt aaagattaca acggttatcc ggcaacgctt tgcatatccg
     8941 taaatgacgt cgttgttcac gggatcccgg gaaaccagga acttaaagaa ggcgatctgg
     9001 tttcgatcga ttgcggtaca gtcattgatg gatattatgg cgattcagcg ttctcgttcg
     9061 ggattggtga gatggacccg gaagtggaga atctgatgaa acggacgaaa gaatcgttgt
     9121 ataaaggcat tgatgttgcc gtggccggta agcggatagg cgatatcgga tatgaagtgc
     9181 aaagctatgt tgaagacttc gggtactcgg tggtacgcga tctcgtaggt catggattgg
     9241 ggaaccatct tcatgaaagg cctgaagtgc cgaactacgg gaaacgggga accggtccca
     9301 aacttgaaga aaacatggtg atatgcatcg agccgatgat aaacctgggc aagcgggtcg
     9361 taatgcagga ccgggacggc tggacaatac gcacgattga tcacaaacct tcggcgcact
     9421 ttgaacatgc tgtcgcaatt cgtaaagaca aagcggatat tctgtcaagt ttcgaaacaa
     9481 tagagcaagt agtaaaccat aacatactga taataaagta agaatggcta aacaactcgt
     9541 aatagaacaa gacggtgtcg tacgagaatc actcggcaat gccatgttca gagtggaact
     9601 tgaaaatgga catgtaatta cagcccacat ctccggaaag atgagaatgc actacataaa
     9661 aattcttccg ggtgataaag ttaaaattga aatgtcgccg tacgatctta ccaaaggaag
     9721 gataattttc cgctacaaat aagaaaactg aataatacta aacaataaat aatacaagca
     9781 atgaaagtca gagcatccgt caagaaaaga acgcctgatt gcaagatagt acgcaggaaa
     9841 gggaggattt atgttatcaa caaaaagaat cccaaattca aacaacggca aggttaatca
     9901 ttgtgtaatc cctaagcagt agaataatat ggcacgtatt gcaggtattg atttaccgaa
     9961 gaataagagg ggcgaaatcg gacttacata catttatggt ataggccgga gcacagccca
    10021 gcacatcctg gataaagccg gcatcgaccg gggtaaaaag gttcaggatt ggaatgatga
    10081 tgaacagaat caaattcgtt caatcatcgg cgaagaaatt aaagttgaag gcgctctccg
    10141 ttctgaggtg cagctcaaca tcaaacgact gatggatatc ggttgttacc gtggtatccg
    10201 ccatcgtctc gggttaccgg ttcgcggcca aagcaccaag aacaatgccc gtacccgcaa
    10261 aggcaggaaa aaaaccgtcg ctaacaagaa aaaggctacc aaaggctaaa aagtaaagat
    10321 taaaagctga aataaaacaa ctatggcaaa aaccaccaaa gtttcgaaga aaaaagtcgt
    10381 aaaagtcgat gcggtaggaa gggcatatgt ttttgcgtcc ttcaacaaca tcatcatctc
    10441 acttaccaac agtacgggcc aggtaatatc atgggcatcg gctggcaaga tgggattccg
    10501 cggttcgaaa aagaacacgc cttatgctgc tcagatggct gctaccgatt gcgccaaagt
    10561 agcttacgac cttggcttgc gtaaagtaaa ggtttacgtg aaaggtcccg ggtcaggcag
    10621 ggagtcggct atccgtaccc ttcatactgc cggtatagaa gttactgaga tccaggatgt
    10681 aaccccattg ccgcacaacg gctgcaggcc tccaaaacgc cgcagggttt aatttgccgg
    10741 aagttcagga ataaaaaaat atcataaaca tatacaaact taacaggcta catggcacgt
    10801 tatattggtc caaagtcgaa aatagcccgc aaattcaagg agccgatcta cggccccgat
    10861 aaggcttacg aaaagaaaaa ctatcctccc ggacagcacg ggcagaataa acgcaggcgc
    10921 aaagtatcgg aatacggtac acagcttgcc gagaaacaaa aagcaaaata tacatacggc
    10981 attctcgaaa aacagttcaa gaataccttc cttaaagcca ccagcaaaaa aggcattacc
    11041 ggtgaaatcc tgcttcaact catcgaagca aggctcgata atgtagttta ccgcctggga
    11101 atcgctccta cccgtgacgc tgcccgccag cttgttagcc accggcacat taccgttaac
    11161 ggaaaagtgg tgaatgttcc ctcgtatgaa cttttacagg gtgatgtagt tgccgtacgc
    11221 gaacgttcaa aatcacttga agtgatccag aacaatatga caggccgcgg cagcaggttt
    11281 tcctggctcg agtgggatga ggcttcgtta acggggaaat ttattaatta cccgcaacgg
    11341 gatgaaattc ctgaaaacat ccaggaacag ctcatcgtcg aattgtattc gaaataatct
    11401 gattcagttt aagttcaacc aacaattagg atatttatgg cgatattatc attccagaaa
    11461 cctgataagg ttatcatggt acaggccgat gagtttgacg gtacctttga gttccgccct
    11521 ctcgaacccg gcttcggcat tactatcgga aatgctttgc gcagaatcct gctctcgtcg
    11581 ctcgaaggtt atgcaattac atcggttcgc attgatggtg ttgaccacga gttctcatct
    11641 atcaaaggtg tggttgaaga tgtaacggat gttgtactga acctaaaaaa gcttcgtttt
    11701 cgcaaacaaa tcgaagcgga agaatccgaa aaagtcaatt tctctttcac aggcaaggat
    11761 atttttacgg ccggtgacat agcaaaacat ctttcggttt ttcaggtgct caatccggat
    11821 gtggtgctct gccacatgga accttcggta aaactcaata tcgaacttac catcaacaaa
    11881 ggcagagggt atgttcccgc cgaagaaaat aaagttccca atgccttaat cggtacaatc
    11941 ttcattgatt cgattcatac tcccatcaag aatgtgaagt ataccattga gaattaccgt
    12001 gttgaacaga aaaccgacta cgaaaagctg gttctcgata ttaaaaccga cgggtcgata
    12061 catccgaaag aagccctgaa agaagctgca aaaatcctca tccaccattt catgctcttc
    12121 agcgacgaaa agatcaccat cgatactgaa gaaaaagccg taacggaaga atttgatgaa
    12181 agcaccctgc atatccgtca actgttgaaa accaaactgg ttgatatgga tctctcggta
    12241 cgcgcattga attgccttaa ggctgctgat gtggaaaccc ttggcgacct ggtagctttc
    12301 aataaaaatg acctgctgaa attcaggaat tttggaaaaa aatcccttac tgaactcgaa
    12361 gaccttgtta aaaccaaggg acttgaattc ggtatgaaca ttgcaaaata caaattagat
    12421 aaggaataag cgaaatgaga catcagaaag caattaacca ccttgggcgg accagcactc
    12481 atcgcaaagc catgctttcg aatatggcaa gctcgctcat caggcacaag cgcataaata
    12541 caacgttagc caaggcaaaa gccctccgca cgtttattga gcccatactc accaaagcca
    12601 aggatgattc aacccattca cgccgtatag cttttagcta tctgcaggat aaagaagcag
    12661 ttacggaact cttccgtacc gttgccgtta aaattgccga ccgtcccggt ggatataccc
    12721 gtatcctgaa aaccggtttc cggcttggcg ataacgccga aatgtgcatg atggagcttg
    12781 ttgacttcaa cgaagtgatg cttgcagcca aagaagcagc gcccaaaaag actacccgcc
    12841 gccgtcgcgg taccggaaag aaagccgaat ccactgctga agccgttgcc gaacctacag
    12901 ccaaggctgc gcccaaggca aaagccaaag caaaagccgc tccggtagct gaaacagaaa
    12961 aaccggaaga accggttgtt gatactgaaa agactgaatc cgatactccg gaagacaatc
    13021 aatagaaaat attcaggaat tggaaaggat gaagtcggat gaactttgtc ctttttttgt
    13081 gttcccaagt ctacacttta aaacagttaa ttatgagtca gatcgcaaac attacagccc
    13141 gccagatcct tgattcgcgc ggcaacccca caatcgaagt ggatgttttc acaacaaacg
    13201 gtatcatggg acgtgcggct gtaccctcag gcgcctccac cggcgttcac gaggctgtcg
    13261 aactccgcga cgaggacaag agtatctata tggggaaagg cgtccttaaa gctgttcaga
    13321 atgtgaataa agtgcttgcc gaagagctga aaggatattt cgtatcggaa cagaacgaga
    13381 tcgacgcccg catgatcgaa ttggatggca ctcccaacaa gggcaacctt ggcgccaatg
    13441 ccattctagg cgtttcgcta gcagtggcca acgcggcagc ccaggaaacc ggacaatacc
    13501 tgtaccggta tatcggcggg gtaaacgcaa acacgctccc catccccatg atgaatattc
    13561 tgaacggcgg ttcgcatgcc gataacagta tcgacttcca ggaattcatg atcatgcccg
    13621 tgggagcccc ttcgttcagc gaagcgcttc gtatgggcgc cgaagtgttc cataacctga
    13681 aatcggtact taaaaaagcc ggctattcca ccaacgtagg cgacgaaggc ggttttgctc
    13741 ccaacctcaa gagcaacgag gaagccatca cggttatcct gcaggccatc gaaaaagccg
    13801 gttacaaacc gggcgccgat gtctatatcg ggctcgatcc cgcttcgagc gaatacttca
    13861 ttcccgaaga aaatgtttat catctccata aatcaaccgg cgataagctt acccccgccc
    13921 aaatggttga ctactggaaa acctgggttg aaaaatatcc gatcatcagc atcgaagacg
    13981 gtatggccga agacgactgg gacggatgga agctgatgac cgatgtattg ggtaagaaaa
    14041 tccagctcgt gggcgacgac ctgtttgtca ccaatgtgaa aaggctgcag atgggcatcg
    14101 aaaaaagcgt tgccaattca atcctcatta aagtaaacca gatcggttcg cttaccgaaa
    14161 ccatcgacgc cgtgaacctg gcttaccgca acagctatac ggccgtgatg agccaccgtt
    14221 cgggcgaaac cgaggatacc accattgccg acctttccgt agccttgaat acaggcctta
    14281 tcaaaaccgg atcggccagc cgtaccgacc gtatcgcaaa gtacaaccag ttgctccgca
    14341 tcgaggaaac ccttgggagc aatgccaggt acct
//
LOCUS       DAOPYV010000072        13659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_4936_length_13659_cov_11.080712, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000072 DAOPYV010000000
VERSION     DAOPYV010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13659
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4936_length_13659_cov_11.080712"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="PKI35_09450"
     CDS             <1..881
                     /locus_tag="PKI35_09450"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNX44648.1"
                     /translation="ERKQSYDNRPYGTIIEETMSHSYKVHPYRWTPIGYPEDINKATL
                     QEFRDFHDTYYVPNNATLSIAGDIDPETAKKLVEKYFGDIPKGTKPMYRPTVAEPPQT
                     AEVRDTVYDNIQLPAVIISYHMPAQGTPDSYALSMLTTLLSDGESSRLSKSVVDKQQK
                     GLEVAAFPFALEDPGLFMIFGLVNMGVTPDVLENAIFEEIEKVKTEDIPDQEFKKLQN
                     KVENDFVTGNSTMAGIAENLSNYRVYFGDANLINTEIERYMKVTKEDLKRVANKYLTK
                     ENRVVLYYLPKPTAQN"
     gene            897..3017
                     /locus_tag="PKI35_09455"
     CDS             897..3017
                     /locus_tag="PKI35_09455"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNX44649.1"
                     /translation="MKTRILAVLFAVLISLPLAAQVSKKQTRKPAATAPAPKEQLDRS
                     KRPVPGPAPVIKIGEAESFVLANGLKVFVVENHKIPRISYQLVLDYDPVLEGENAGYV
                     DFAGQLMRTGTTTRTKDQIDEAIDFIGARLSTSADGLSASCLTRHNEALLDIMSDIIK
                     NASYKAEELEKIRTQTISALEAEKNEPSSISEKVGSRLIFGKTHPYGEFQTLASVKNI
                     TLEKCEEFYHTYYKPNIGYLAIVGDITPAQAKSLAEKYLGDWQQGEVPKARYLTPKPP
                     LKNTVAIVDRPNAVQSTIKVCYPLDLKPGSPDEMGARVMNTILGGGSFRLFNNLREKH
                     GWTYGAYSQMRSDRLAAQFNASAEVRNPVTDSAYTQILFEMNRLRNEPVGNEELAMIK
                     NYLNGNFGRSLENPGTVANFAINTARYNLPKDYYATYLSRLDAINATDVQMMAQKYLR
                     PDNTWLLTVGKAEDIAPKLKAFSKTGEIQYYDIDGNSYDPSKKLKPAPAGITAETVIN
                     SYITAIGGEKKIRKIKDATIQASATMQNMPIDMAMYYKLPGKFLMQIGSGSMIFGKQI
                     YNTGRGVAITPMNGETKAVEGDDLQMMKEQASLIPELNYAEFGTKAELLGIEESDGKE
                     YYKVQITRSSGKKETSYYDIATSLKMKTESEMESAEYGDYKPVNGVMFPFSLTQVAGP
                     ETIRFTVTSLTTNTKIKDDLFEIK"
     gene            3055..3585
                     /gene="rimM"
                     /locus_tag="PKI35_09460"
     CDS             3055..3585
                     /gene="rimM"
                     /locus_tag="PKI35_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNX44650.1"
                     /translation="MMDRSDLFELGKVLKTFGSKGEVIIHLDVDTPDKYRKLDSVFIR
                     FNKSLIPFFIESIQIKSKNQAIVKFQDVDTQDDAAMFSGCSLFLPLSKLPKLSGNKFY
                     YHEIVGFSVVDTQKGNIGTVKRILELPTQAMFEIEYGHKEILVPVVDEIIVKVDRKNR
                     ILRIDAPEGLIDLYLE"
     gene            3621..4319
                     /locus_tag="PKI35_09465"
     CDS             3621..4319
                     /locus_tag="PKI35_09465"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNX44651.1"
                     /translation="MFRFKQFCLSDENATMKTGTDAVLLGSWAGVTGCRRILDIGTGS
                     GIIALMLAQRCEAMVDAIEIDEASALQAENNVHQSPWPDRIKVHHTSFQAFAASTTER
                     YDAVISNPPFFSRALKAPDPVRCRARHDDELPAGELIRDAVKILLTGGKIHLVVPFRD
                     LKKWETEASFQQLYCINVTHVIPKEGKPPFRALVTFSGKPGSLIKESLVIHNSDGSYT
                     DDYRNLTKDFYLAF"
     gene            4392..4736
                     /locus_tag="PKI35_09470"
     CDS             4392..4736
                     /locus_tag="PKI35_09470"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HNX44652.1"
                     /translation="MKTARTFFLLSFILLWVTNGYAQKIYSCNSRYDADVKIFVTDSR
                     YDADLLVYKVGSRYDADGNEGRWFFVDSRYDADKKVYFTDSRYDADLLIFFVDSRYDA
                     GWRNKEKIHLLY"
     gene            4785..5564
                     /locus_tag="PKI35_09475"
     CDS             4785..5564
                     /locus_tag="PKI35_09475"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNX44653.1"
                     /translation="MRSRFRFSGIIILTMTLSAGCSILKPTPLRVAISSSSENYENWI
                     KRTEPSAVLINFKGMEAGPAGSLLESCDGLLLTGGVDVEPALYGKTCDTSLCKTDPER
                     DALEMALIRKAINLQIPVIGICRGQQILNVATGGTLFTHIPADCPSTVIHRSEDNANC
                     LHQVKIDTASNLNKICQVSGGLVNSSHHQAVEVTSDVFKAVAWSDDGIIEAIEYVNPE
                     GKPYLQAVQWHPERMDPSSPLSTTLMKSFITAMEESRKAKK"
     gene            complement(5571..6086)
                     /locus_tag="PKI35_09480"
     CDS             complement(5571..6086)
                     /locus_tag="PKI35_09480"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX44654.1"
                     /translation="MPERNSQAALLADKLELLPHPEGGWYREVYRSDEKIDGEALPSR
                     FAGDHTYCTSIYYLLESGDFSAFHRIKSDETWHFYLGSPLKLYILREDGTLLTVMLGD
                     CVDTGHVFQWTVPRGQWFAAEVSVPGSFTLMGCTVSPGFEFDDMEMASFCKLESRYSR
                     YATLIRRMTRM"
     gene            complement(6070..6744)
                     /locus_tag="PKI35_09485"
     CDS             complement(6070..6744)
                     /locus_tag="PKI35_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNX44655.1"
                     /translation="MKNAIFSSLIFVLLISLMPAKAQLFRGELIAGMNKSQVDGDEAY
                     RFKKYGLNFGAGVAVPVYKNWFASMELLYNQKGAYKKAISNDTMNGSYRLNLDYVEIP
                     VLFYYTDKEFISAGAGFSWGNLISVKEAEKRYSIDPIDLDTVYHKSDWNAIADFRVRI
                     YKNLKLNVRYAYSIKNIGVRDVRDSESGLWIHNDKQFNNLWTFRLIYMINEKPPVKKK
                     KDAGTE"
     gene            complement(6982..9126)
                     /locus_tag="PKI35_09490"
     CDS             complement(6982..9126)
                     /locus_tag="PKI35_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNX44656.1"
                     /translation="MDSRFITQIANELGIREFQVLNTINLLKDGATVPFISRYRKEMT
                     GSLDEVQVAAIRDRYDQILELEKRREAILNSIREQEKLTPELEKQIMEAGTMSELEDI
                     YLPYRPKRRTRAMIARERGLEPLADLVFAQLYFDLDAKAKTFINLEKEVNTVEDALSG
                     ARDIVAEWVNEDKGSRERVRNLFSKKAAISSRAVRGKELEGIKYEAYYDWSESLLKAP
                     SHRLLAMLRGENEGFLKVSIEPTEEEALEILNRRFVRANNAAAQQVETAVKDSYKRLL
                     QPSMETEFRQIAKEKADKEAIRVFAENLRQLLLAPPLGQKNVLAIDPGFRTGCKVVVI
                     DRQGRLLHNETIYPHPPQNEFKPAAAKIQNLVKAYKIEAIAIGNGTAGRETENFIKRI
                     PFDTNILALMVNESGASVYSASAVAREEFPEYDVTVRGAVSIGRRLMDPLSELVKIDP
                     KSIGVGQYQHDVNQPLLQKGLEDAVISCVNKVGVELNTASKHLLSYVSGIGPSLASKI
                     VEFRDKNGVFHSRKELLKVPRFGDKAFEQAAGFLRIRESENPLDRSAVHPESYIIVDH
                     MAEKLGCSLNELMSSDELRKKINLRDFVSGNVGLPTLNDIMQELAKPGRDPREKFDFF
                     EFDKNVNAIEDVLPGMVLPGIVTNITNFGAFVDIGVHQDGLVHVSQLTNRFVKDPNEV
                     LKLNQKVMVKVLVVDKDRKRIQLSMKDVQQDA"
     gene            10117..11211
                     /locus_tag="PKI35_09495"
     CDS             10117..11211
                     /locus_tag="PKI35_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="HNX44657.1"
                     /translation="MANSNLTNAKNAKNDEFYTQYHDIEKEINAYLEFNPDVFRDKTI
                     LLPCDDPEWSNFTKFFAQNFQRFGLKKLISTSYAADSKKYKSSYQPTLFEVNDPQFDE
                     YKTTRYGKIFTLTHDKSGDGKVDVNDLVWHYLAGDGDFKSAEIKKLRNEADIIITNPP
                     FSLFREFLAWIVEADKQFIIIGNLNAIIYKEVFPLIKENKVWLGATGNGNDMVFAVPP
                     GTEVAESDRQKAARLGYVGDYTRLGNSCWFTNLDHGRRHQALPLMTMSDNLKYSKHKE
                     IKGKESYDRYDNYDAIDVPFIDAIPSDYDGVMGVPVSFLDKYNPEQFLLLGSNRGIDQ
                     DPKGIYGRGSYFNGKETFKRIFIKHKSATK"
     gene            11627..12076
                     /gene="tnpA"
                     /locus_tag="PKI35_09500"
     CDS             11627..12076
                     /gene="tnpA"
                     /locus_tag="PKI35_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNX44658.1"
                     /translation="MSYTRLLYHIVFRTKYGNYTIPEQYEKELYAYMMGIISNKKSKL
                     YRIGGVGNHIHLLVDIHPTIAVSDFMKELKEYSSKWLKTNPNFPDFKGWAVSFAAFTY
                     NLNDKQMIINYIKNQKEHHKKVSFENEYRNFLVENGIEIDERYFLKD"
     gene            12190..13353
                     /locus_tag="PKI35_09505"
     CDS             12190..13353
                     /locus_tag="PKI35_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNX44659.1"
                     /translation="MKTDLKTDITVAAICDGFVYNELEGKGLFGLSGKLTIQPEYQRN
                     YIYADGKKDVAVIESLLKGYPLGLIYFVKVNAHKFEVLDGQQRITSFGRFVTNKFAVK
                     DENGMEQYFSGIAADKQARILETKLTIYECEGTESEIKQWFRTINIAGIPLNDQELLN
                     AIFSGPFVTLGKEEFSNSQNSNIQKWSAYVSGSANRQEFLECALHWVSRGNISDYMSR
                     HRFDTNINELKTYFNTVIEWVSGVFTDVLSEMRGLEWGQLYEKYHCTAYNPAKVSKEV
                     QKLYADPYVRNRKGIFEYILGGSVDTKLLDVRVFDDATRKAVYASQTAQAETKGESNC
                     PLCALGHDANKNKIWKSADMDADHVSAWSNGGTTNIKNCQMLCKTHNRAKGNR"
BASE COUNT         3888 a         3350 c         3080 g         3341 t
ORIGIN      
        1 aagaacgcaa gcagtcgtac gacaacaggc cttacggaac gatcatcgaa gaaaccatgt
       61 cgcattctta caaagttcat ccttaccggt ggacacccat aggatatcct gaagatataa
      121 acaaggccac tttacaggaa ttcagggact ttcacgacac ttattatgtt cccaacaatg
      181 ccacgctctc cattgccggc gatattgatc ccgagaccgc caaaaaactg gttgaaaagt
      241 attttggcga tattcccaaa gggacaaagc ccatgtaccg gccgacggtt gcagaaccgc
      301 ctcagacagc cgaagtacgc gacacggtat acgacaacat ccaactgcct gccgtaatta
      361 tttcctatca catgccggca cagggtacgc ctgactcata cgcgctttcg atgctcacga
      421 ccctgctttc ggacggcgaa agttcaagac tttccaaatc ggttgtagat aaacagcaga
      481 aaggccttga agtagcagct ttccccttcg cccttgaaga tccggggtta tttatgatat
      541 tcggacttgt taacatgggg gttacacccg atgtgctgga aaatgccatc ttcgaagaaa
      601 ttgaaaaagt aaagactgaa gatattcccg accaggaatt caaaaagctt cagaacaagg
      661 ttgaaaacga tttcgtaacc ggcaacagca ccatggccgg catagccgaa aacctctcga
      721 attaccgtgt ttacttcggc gatgccaatc tgatcaatac cgaaatcgaa aggtatatga
      781 aagtcacgaa ggaagacctg aaacgggtag ccaacaagta tctcacgaag gaaaaccgtg
      841 tagtcctcta ttatttaccc aaacctactg cccaaaacta aaggaggaaa cgaaacatga
      901 aaacaagaat attagccgta ttatttgccg tactgatctc gttgcctctt gcagcccagg
      961 tatcgaaaaa acaaaccagg aaacccgctg ccactgcccc tgctcccaaa gaacagctcg
     1021 acaggagcaa acgccccgta ccgggacctg ctcctgttat caagatcggt gaagcggaat
     1081 cgttcgtact tgccaacgga ctgaaagttt tcgttgtcga aaaccataaa atcccgcgca
     1141 tctcttatca gcttgtcctc gattacgacc cggttctcga aggcgaaaat gccggatatg
     1201 ttgattttgc aggacagctc atgcgtaccg gaacaaccac ccgcaccaag gatcagattg
     1261 atgaagccat cgattttatt ggagcaaggc tttcaacatc cgccgacggt ttatccgcat
     1321 cctgcctcac aagacataac gaggcattac tcgacatcat gtccgatatc atcaaaaacg
     1381 catcctataa agctgaagaa ctggagaaaa tccgcacaca aaccatctca gcccttgaag
     1441 cagaaaaaaa cgaaccttcg tcaatttccg aaaaggtcgg aagccgtctg atattcggga
     1501 aaacacatcc ttacggtgaa ttccagacct tagcctcggt aaagaatatc accctggaaa
     1561 aatgtgaaga attctatcat acctattaca agccgaacat cggctatctg gccattgttg
     1621 gtgatattac gcctgcacag gcaaaaagcc ttgccgaaaa atacctcggc gactggcaac
     1681 agggagaagt gccaaaggcc aggtacctga cgcccaaacc tccgttgaaa aatacggttg
     1741 ccatcgtcga caggccgaat gccgttcaat caaccatcaa ggtttgctac ccgcttgacc
     1801 tgaaacccgg ttcaccggat gaaatggggg caagagtcat gaataccatc ctgggtggcg
     1861 gatcattcag gcttttcaac aacctgcgtg aaaaacacgg ttggacatac ggcgcttatt
     1921 cacaaatgag gagcgaccgg ctggcagccc agttcaatgc atctgccgaa gtccgcaacc
     1981 ctgttaccga cagcgcttac acacagatac tttttgaaat gaaccgcctg aggaatgaac
     2041 cggtcggtaa cgaggagctg gcaatgatca agaactacct caacggcaac ttcggacgtt
     2101 cgctcgaaaa cccgggcact gtggctaatt ttgccatcaa caccgcccgt tacaatcttc
     2161 ccaaagatta ttatgcaact tacctgagca ggcttgatgc cattaatgca actgacgttc
     2221 agatgatggc ccagaaatac cttcgtcccg ataatacctg gttgctcacg gtagggaaag
     2281 ccgaagatat tgccccgaaa ctgaaagcct tcagcaaaac cggtgaaatt cagtattatg
     2341 acattgacgg taattcgtac gacccgtcga aaaagctgaa gcccgcacct gccggcatta
     2401 cggccgaaac tgtcatcaat agctacatca cagctatcgg cggagagaaa aaaatcagga
     2461 agatcaagga tgcaactatc caggcttcag caaccatgca gaatatgccg atcgacatgg
     2521 ccatgtacta taaactgccg gggaaatttc tcatgcagat aggttcgggt tcaatgatat
     2581 tcggaaaaca gatctataat accggcagag gggtcgctat tacgcccatg aacggtgaaa
     2641 cgaaagctgt tgaaggggat gatctgcaaa tgatgaaaga acaggcctcc ctgatcccag
     2701 aactgaatta tgccgaattc ggaacaaagg ccgaattact cggcattgaa gaatcggacg
     2761 gaaaagaata ttacaaagtt cagatcaccc ggtcgagcgg aaagaaagaa accagttact
     2821 atgacattgc taccagcctg aaaatgaaga ccgaatccga aatggaatcg gccgaatacg
     2881 gcgattacaa accggtaaac ggagtgatgt tcccgttctc attaacccag gttgcagggc
     2941 ctgaaaccat ccggttcacg gttaccagcc tcaccaccaa tacaaagatt aaagacgacc
     3001 tgttcgagat caaatagcct tcactattta tcccggaaaa cccataaaac aaagatgatg
     3061 gaccgttcag acctcttcga actgggaaaa gtgctgaaaa cctttggttc gaaaggcgag
     3121 gtcattattc atcttgatgt tgacacacct gataaatacc ggaaactcga ttcggtattt
     3181 atcaggttta ataaaagcct gatccctttt ttcatcgaat cgatccagat caagtctaaa
     3241 aaccaggcca tcgtcaaatt ccaggatgtc gatacccagg atgatgcggc gatgttttcc
     3301 ggttgcagcc ttttcttgcc actttccaaa ctcccgaaac tatcgggcaa caagttttat
     3361 tatcatgaaa tcgtcggttt cagtgtagtc gatacccaaa aaggcaatat tggcacggtg
     3421 aaacgcatcc tcgaattgcc cactcaggcc atgtttgaaa ttgaatacgg ccataaagaa
     3481 atcctcgtgc cggtggttga tgagatcatc gtgaaagtag accggaaaaa ccggatactc
     3541 agaatcgatg cgccggaagg tctgatcgac ctctatctcg aataaccggc agaccggact
     3601 tttccctaat ttccgactgt atgttcaggt tcaagcagtt ctgcctgagt gatgaaaacg
     3661 ccaccatgaa gaccggcacc gatgccgtat tgctgggatc atgggccggc gtaaccggat
     3721 gcagacggat acttgacatc ggcacgggtt ccgggatcat cgccctgatg ctggcacagc
     3781 ggtgcgaagc tatggtcgac gccatcgaaa ttgatgaagc ttcggcgttg caggctgaaa
     3841 acaacgttca tcaaagcccc tggcccgaca ggattaaagt gcatcacacc tccttccagg
     3901 catttgcggc ttccacaacc gagcgttatg atgccgtgat aagcaaccct ccttttttca
     3961 gccgtgcgct aaaagccccc gatccggtgc gatgccgcgc ccggcacgat gatgaactgc
     4021 ccgccggcga actgatcaga gatgccgtaa aaattctgct caccggaggt aaaatacacc
     4081 ttgtggtgcc attcagggac ctgaaaaaat gggaaacgga agcatcgttt cagcaattgt
     4141 attgcattaa cgttacacat gtgatcccga aagaagggaa gccgccattc cgggcgcttg
     4201 taacgttttc cggaaagccc gggtccctga tcaaggaatc actggtaatc cataacagtg
     4261 acggaagcta taccgacgat tacaggaacc tgaccaaaga tttttatctt gccttctgat
     4321 gaaatgcttt aattttaacc ggttcataca aatagtgaag cctctttgag ctgccatatc
     4381 aaccgaagat catgaaaacc gccaggactt tctttttgtt atcgtttata ctgctctggg
     4441 taaccaacgg ttatgcacaa aaaatttaca gttgcaacag ccggtacgat gccgacgtaa
     4501 aaatatttgt aacggatagc cgttacgatg ccgacctgct cgtgtataaa gtcggcagcc
     4561 ggtacgatgc cgacgggaac gaaggccgat ggttttttgt cgacagccgc tacgatgccg
     4621 ataaaaaagt atattttacc gacagccgtt acgacgccga cctgctgatc ttttttgtcg
     4681 acagccgtta cgatgcaggc tggagaaaca aagagaaaat ccacctgctc tattgatctt
     4741 caatctaact caatactaga aaccttaaag ggaaacctga gaatatgagg agccgtttca
     4801 gattttcagg aatcatcatt ctgaccatga ctctttcagc cggatgttca attctgaagc
     4861 cgacgcccct gcgtgttgcg atttcgtcct catcggaaaa ttatgaaaac tggataaagc
     4921 gaacggaacc ttcggccgtt ctcatcaact ttaaaggcat ggaagccggc ccggccggat
     4981 cgttgctgga atcgtgcgac ggcctgttgc tgaccggcgg cgtggatgtt gagcctgccc
     5041 tttacggcaa aacatgcgat accagcctgt gcaaaaccga ccctgaacgc gacgcccttg
     5101 aaatggccct gatacgtaaa gcgataaatc tgcagatacc ggtaatcgga atatgccgcg
     5161 ggcagcagat tctgaacgta gcaacgggcg gaacgctgtt tacgcacatc cctgccgatt
     5221 gcccgtcaac ggtaatccac cggagtgaag ataacgccaa ttgccttcat caggtaaaaa
     5281 tcgacaccgc ttcgaacctg aataaaatat gccaggtttc cggcggcctc gtaaattcaa
     5341 gccatcacca ggctgtagaa gtgacttcgg acgtctttaa ggctgttgcc tggtcggacg
     5401 acggcattat cgaagccatt gaatatgtca acccggaagg caaaccttat ttacaggctg
     5461 ttcaatggca tcccgaaagg atggatccat ccagcccttt atcgaccact ttaatgaaga
     5521 gttttattac ggctatggaa gaaagccgga aggcaaagaa ataagcccgg tcacattcgc
     5581 gtcatccgcc ggatgagcgt cgcataccgt gagtaccggc tttcgagttt acagaacgag
     5641 gccatctcca tatcgtcaaa ttcaaatccc gggctgacgg tacatcccat cagcgtaaac
     5701 gaacccggga ccgaaacttc agcagcaaac cattgcccgc gggggacggt ccactgaaat
     5761 acatgaccgg tatccacaca atcgcccagc atcaccgtaa gcagggtacc atcttccctg
     5821 agaatgtata gtttcagggg gcttcccagg taaaaatgcc aggtttcatc agactttatc
     5881 ctgtgaaagg ccgaaaaatc accgctttcg agaagataat aaatggaagt gcaatacgta
     5941 tgatcaccgg caaagcgcga cggaagcgcc tcaccgtcaa tcttttcgtc cgaacgatag
     6001 acttcacggt accatccgcc ttcggggtga ggcaataatt ccagtttatc cgccagcaaa
     6061 gcagcctgac tattccgttc cggcatcttt ctttttcttt acgggaggct tttcattgat
     6121 catatagatc agtctgaaag tccagaggtt gttaaattgt ttatcgttat gaatccacag
     6181 ccccgactcg ctgtcgcgca catcgcgtac gccaatattt ttgatggaat atgcgtacct
     6241 gacgttcagt ttcaggttct tgtaaatcct gacacgaaaa tcggcaatgg cgttccagtc
     6301 ggatttatgg taaacggtat ccagatcaat cggatcgatg gaataccttt tctccgcttc
     6361 tttcacactg atcaggtttc cccacgaaaa accggcgccg gcgcttataa attccttatc
     6421 ggtataataa aacagcaccg gaatttccac ataatccagg ttgaggcgat acgatccgtt
     6481 catggtatcg ttgctgatgg cttttttgta tgcacctttc tggttgtata aaagctccat
     6541 cgaagcaaac cagtttttat aaaccggaac agctacaccg gccccgaaat tcaggccata
     6601 ctttttgaaa cggtatgctt catcgccgtc aacctgcgat ttattcattc ccgcaatgag
     6661 ttccccgcga aagagctggg cttttgcagg catcaggctt atgagtaaaa caaagataag
     6721 gcttgaaaat attgcatttt tcatgttgca aaggtagtaa ttagaccgtt gggaaattca
     6781 gggagacaat cacaggaaaa cggttatcag gcggataaat tgtccggagg cctgattatc
     6841 gctgatggta aggaggaatg aaaccgggac gtaaatggtc ggcttttggt ccggtaaggc
     6901 tcaccgtcag ttcctctctg cctggtgtac tatttacgca tcaaattccc cgttcaccgg
     6961 agtcaggaaa cgcccaacct ctcaggcatc ctgctgcaca tctttcatcg acagttgaat
     7021 ccgcttccgg tccttgtcca ccacgagtac tttcaccatc actttctggt tgagtttcag
     7081 cacttcattg ggatctttca caaaacggtt tgtgagctgg ctcacatgaa caagcccgtc
     7141 ctggtgtacc ccgatatcga cgaaggcccc gaaattggtg atattggtta caattccggg
     7201 aagcaccatg cccggcagca catcttcgat ggcgttgaca tttttatcga attcgaagaa
     7261 atcgaatttc tcgcgcgggt cgcggcccgg tttggcaagc tcctgcatga tatcgttcag
     7321 tgtggggagg cctacgtttc cggatacaaa atcgcgaagg ttgatctttt tcctgagttc
     7381 atcgctgctc atcagctcgt tgagggaaca gcccagcttt tcggccatat ggtccacgat
     7441 gatatagctt tcgggatgta cggcgctgcg gtcgagggga ttttccgatt cacggatgcg
     7501 aaggaatcct gcagcctgct cgaaggcttt atcgccaaaa cgcggcacct tcagcaactc
     7561 tttccttgaa tgaaaaacac cgtttttgtc cctgaactcc acaatttttg aagccagcga
     7621 cggcccgatg cccgaaacat aactcaacag gtgcttgctg gcggtattca actccacccc
     7681 taccttgttc acgcaactga tcaccgcatc ttccagccct ttttgcagca acggctggtt
     7741 tacatcatgc tgatactggc ccaccccgat ggatttcgga tctatcttca ccagttccga
     7801 caaagggtcc atcagacgac gtccaatgga aacagcgccc cttaccgtga cgtcatattc
     7861 aggaaattct tcgcgggcga cagccgaagc ggaatatacc gaagcgccgc tctcgtttac
     7921 catcaatgcc agaatgttgg tatcgaaagg aatgcgcttg atgaaatttt cggtttcgcg
     7981 gcccgccgtc ccgttaccga tggcgatggc ttcaatttta taggctttta cgagattctg
     8041 aattttcgct gccgccggtt tgaattcgtt ttggggaggg tggggataaa ttgtttcgtt
     8101 gtgcaaaagc ctcccctgcc tgtcgataac cacgaccttg catcccgtgc ggaaccccgg
     8161 gtctatcgcg aggacattct tttgccccag cggcggcgcc aacagaagct gacgcagatt
     8221 ttcagcgaaa acgcggatgg cttccttatc ggccttctcc ttggctatct gccggaattc
     8281 cgtctccatc gaaggctgca acaaccgctt ataggaatcc ttcacggccg tctccacctg
     8341 ttgggcggca gcattgtttg ccctgacaaa tctccggttc agtatctcga gggcttcctc
     8401 ttcggtcggt tcaattgata ccttcaggaa cccttcattt tcacccctta gcatggccag
     8461 caacctgtgt gaaggggctt tcagcaacga ttcgctccag tcgtaatagg cttcgtattt
     8521 gataccctcc agttctttgc ccctcacggc ccttgacgaa atggcagctt tcttcgagaa
     8581 aaggttcctg accctttcgc ggctgccctt atcttcgttt acccactccg ctacgatatc
     8641 gcgcgccccc gaaagggcat cttccaccgt atttacttcc ttttcgagat tgataaaagt
     8701 cttcgctttc gcatccaggt cgaagtacag ttgggcgaaa acaaggtctg ccagaggttc
     8761 gaggcctctc tcacgggcta tcatggcacg ggtgcggcgc ttgggacgat agggaaggta
     8821 aatatcttcc agttcgctca tggtgccggc ttccataatc tgcttttcca gttccggcgt
     8881 cagcttttcc tgttcccgga tggaattcag gatggcttcg cggcgttttt ccagttccag
     8941 gatctgatcg tacctgtcgc gtatagctgc cacctgcacc tcgtccaggc tgcctgtcat
     9001 ttccttccgg taacgcgaaa taaacgggac cgtggcgccg tccttcagaa gattaatcgt
     9061 attcagtacc tgaaattccc tgattccaag ctcattggct atctgtgtta tgaaacggct
     9121 gtccattttt ttgtattata tcctgaaaac aaaaccggta taaaaataca aaaagccgcg
     9181 gtactggcgg actttgatga taacctttcc gtcagtctcc gctcgcctct cctgtcgagc
     9241 gcgctattta ttccgatcta tgctttcccc ctgacgtata cgagtggagg agaaatgaaa
     9301 ggagaggaga acgcacagga gtactcagga tttttttaac cacggagcca cggagaacag
     9361 gaagaaacac agagatttac attattctca gtgaatctca gtgacctacg ctcgcgtgtg
     9421 catcctgacg cgttcgttga aactttacaa tgctatacaa aatttgtttt cttttattgc
     9481 atgaccgata taacatatct gaggaataag tgtatgagaa tagtgtcgca catgtcaggg
     9541 aacgcgcgag ccagggcaaa acggcggacg taatcctgcc tttcaggcag actgacggat
     9601 gctgtttgta tcatcatcag gccgatggcc tcaggttatg aaagtctgtc ctttcaggac
     9661 attacgacat gacctcgaag aggtctgact ttcatgactg cagggcttcg gcctgcggtt
     9721 aaaagctaaa gaaccaacga ctgcctgaaa ggcaggatgt aaacctccgc gcgcctctcc
     9781 tgacgtgtgc gttgaaactt tacaatgcta tataaaattt gtttactttt attgtatgat
     9841 tggaatatga aatgtctgaa aaataagtgg atgagaataa gtggcgcaca cgtcaggaga
     9901 cgcgcgccaa ccaagggaat tacctaccga tgatggtgga ttgctatccg accatttcga
     9961 attgattgcc gactgttgca tcaaataatt aaatcaataa ccaaaatcaa taaaattgtt
    10021 aagtttgtac tgaagattcc aggttatatc cttcgaaaca ctcataataa agcataaagc
    10081 taattgtagc acaatttatt gaaaccataa taacaaatgg ccaacagcaa cctcactaat
    10141 gccaaaaacg ctaaaaacga cgagttctat acacagtatc acgatatcga aaaggaaatc
    10201 aatgcctatc tggaattcaa ccctgatgtg ttccgtgaca aaacgatact gctgccttgc
    10261 gacgaccccg agtggagcaa ttttactaaa ttctttgccc agaattttca gcgctttggc
    10321 ctaaaaaagc ttataagcac cagctatgcc gccgacagca aaaaatataa aagcagttac
    10381 cagccaacgc tattcgaagt caacgatccg caattcgacg aatacaaaac caccaggtac
    10441 ggtaaaatat ttaccctcac ccacgacaag tcaggcgatg gcaaagtgga tgtaaatgac
    10501 ctggtgtggc attacctggc aggcgatggc gattttaaaa gcgctgagat caaaaaactg
    10561 cgcaacgaag ccgatattat cattaccaac ccgcctttct cgttgtttcg tgaattttta
    10621 gcgtggattg ttgaggctga caaacaattt ataattattg gaaatttgaa tgcaatcatt
    10681 tacaaagagg ttttccccct aattaaagaa aacaaagtgt ggttaggggc gacaggaaat
    10741 ggcaatgata tggtttttgc cgttcccccc gggacagaag ttgctgaaag tgacaggcag
    10801 aaagcggccc ggcttggtta tgtgggggat tatacaagac tcggaaattc gtgttggttt
    10861 acgaaccttg atcatggtcg gcgtcaccaa gcattgccac taatgacgat gagtgataat
    10921 ttgaaataca gcaagcataa agagattaag ggtaaagaat cgtatgacag atatgacaac
    10981 tatgatgcta tagatgtccc gtttatcgat gcgataccaa gtgattatga tggagtgatg
    11041 ggtgtacctg ttagttttct tgataaatat aatcctgaac agtttctttt attgggttca
    11101 aatcgaggca tcgaccaaga cccaaaagga atttatggac gtggttctta ttttaatggc
    11161 aaagaaacat ttaaaagaat ttttattaaa cacaaaagtg caaccaaatg ataacaacgc
    11221 taaaaaccga catcatggta gccgctatgt aaggaatttg atacttgcga aacaggatgc
    11281 aacaacttcg aagaggtcag gccttgttaa cagcaaggca taacagcaga caaaatcctg
    11341 cctttcaggc aggcaggcgg atgctggttg tatcatcatc aggccgctgg ccttgggtta
    11401 tgaaagtctg tccttccagg accaatgaaa gtcaaatcct cataaagata acgagaatac
    11461 tataaacgga tattacaaag cagttaattg agaaggattt ataagatgcg aattgtaaaa
    11521 ctacgacctc gaagaggtct gacattcata acggcagggc ttcagcctgt ggttataagc
    11581 gaaagaaccg acaactgcct gaaaggcagg acataaacac caaactatga gttacacacg
    11641 gctattatat cacatagtat tccgcaccaa atatggaaat tataccattc cggagcaata
    11701 cgaaaaagag ttgtatgcat acatgatggg cattatcagc aataaaaaat ctaagttata
    11761 tcgtatcggt ggcgtcggaa atcacattca cctgctggtt gacattcatc caactattgc
    11821 agtctctgat ttcatgaaag aactcaaaga atattcaagt aaatggttga aaacaaatcc
    11881 aaatttcccg gattttaaag gttgggcagt aagttttgca gcatttacct ataacctgaa
    11941 cgataaacag atgataataa actatataaa aaaccaaaaa gaacatcata aaaaagttag
    12001 ttttgaaaat gaatatcgga atttcctggt tgaaaacggt attgagatag atgaacggta
    12061 ttttttgaaa gactgaatcg gtacttcctt taaggcagag ggtaatattc agataatcat
    12121 catcccattc gccttaagta aataaaattt gtcccttagg gactcactaa caaataaata
    12181 ctacaattaa tgaagacaga ccttaaaacc gatatcactg ttgcagctat ttgcgatgga
    12241 tttgtatata acgaacttga aggcaagggc ttgtttgggt tatccggcaa attaaccata
    12301 caacccgaat accagcgcaa ctacatatat gccgatggta aaaaagatgt agcagtaatc
    12361 gaatcactgc taaaaggcta tccgctgggc ctgatttatt ttgttaaagt aaacgctcat
    12421 aagttcgaag tcctggacgg gcaacaacga ataaccagtt tcgggcggtt tgtaaccaac
    12481 aaatttgccg taaaggacga aaacggcatg gagcagtatt tcagcggtat tgcagccgac
    12541 aaacaggccc ggattcttga aaccaagcta accatttacg aatgcgaagg taccgaaagc
    12601 gaaataaagc aatggtttcg taccatcaat attgccggta tccccctaaa cgatcaggaa
    12661 ttgctcaacg caattttctc aggaccgttt gtaacactcg gcaaggaaga gttcagcaac
    12721 agtcagaact ccaatatcca gaagtggagt gcctatgttt ccggcagcgc caaccggcag
    12781 gaattccttg aatgcgccct ccattgggtg agcagaggca acatcagcga ttatatgagc
    12841 cgtcaccgct tcgataccaa cattaatgag ttaaaaacgt atttcaatac ggttatcgaa
    12901 tgggtttcag gcgtgtttac cgatgtgctc agcgagatgc gcggcctcga atggggtcaa
    12961 ctgtacgaga agtaccactg cacagcttac aacccggcaa aagtaagtaa agaagtacaa
    13021 aaactctatg ccgacccgta tgttagaaac cgtaaaggca tcttcgaata catccttggc
    13081 ggttcagtag ataccaaatt gctcgatgtc cgtgtttttg acgacgccac caggaaagcc
    13141 gtttacgctt ctcagacggc acaagccgaa accaaaggag aatcaaattg tccgctttgt
    13201 gcccttggcc acgatgccaa caagaataaa atctggaaat cagccgacat ggatgccgac
    13261 catgtatcag cctggagcaa cggcggcaca accaatatta aaaactgcca gatgttgtgc
    13321 aaaacacata accgggctaa aggaaaccgg taatagtact tttttactat taccttcact
    13381 cacgcgtctc ctgacgcctt cgttgaaaga tgtccttctt accaagggca cagaggccac
    13441 cgaggttcac tgagaacgct ataaagctct gtgttccttc gtgttctccg tggctccgtg
    13501 gttttttaac tccgtcttcc gccctccgtc ctccttgctc cgttctccct gctccccgct
    13561 ccctgccccc tgcttacctt atcagcgtaa tggttcccca ttctgcatcc tggatatcgt
    13621 cgcgtttgcc ggtatcctgg ttatgccata tcgtgccgt
//
LOCUS       DAOPYV010000073        13306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_5126_length_13306_cov_12.066712, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000073 DAOPYV010000000
VERSION     DAOPYV010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13306
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5126_length_13306_cov_12.066712"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..702)
                     /locus_tag="PKI35_09510"
     CDS             complement(136..702)
                     /locus_tag="PKI35_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44660.1"
                     /translation="MKKLILIIINNLIILSFAFAQTKIDYHELTSEVNEYIENVNKFR
                     NRPNDTSEMIVIMSLNKVSDDTIIIEFKRVIIDYEVFNADFQYISKEGKSYLLIKLNN
                     ISLNEILDNISGIQIFHKNEKNEILKKLYDSSKRMVDHRPLIVFLTYNRKYGFFTSKE
                     YFFSFLPENYGTIEDKNWEFIMYPNPKK"
     gene            complement(1014..3929)
                     /locus_tag="PKI35_09515"
     CDS             complement(1014..3929)
                     /locus_tag="PKI35_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNX44661.1"
                     /translation="MKKLSTLAISLLLVVFATANPVTIEKARLVADNYFRHYSGKSEL
                     TLKESSVIEYKGTITCFIFNYEGGGFVVVSADDAAVPVLAQSNTGNFPAEFSQPNLKY
                     WFDGYNQEIKGAVDSRLSNEYTIGSWNKLLNNNFDRGTNDVDPLVTTSWDQGAWYNYY
                     CPAASGGQGGHVWAGCVATAMGQIMKFHNFPEQGVLSHSYTHPTYGQQTANFGATTYN
                     WAQMPTQATSSNYQSIATLLYHNGVAVDMNYGIDGSGAQSSDVPWALNTYFNYDPSTI
                     SLVYKADYSLTEWKNILIEDLTASRPIYYAGDDGSSGHAWVCDGYRSSDSKFHMNWGW
                     SGSGNGYFTIGALNPPGYSPNQNNHAVIGIQPYNPDLIVRITNMGPQQIVPYRSAFDI
                     NCSVEKGTATTVNLYIDDNIVYTSAQASFVYSWPTAAYALGSHTVKVVALNETDTVYH
                     QVSVGLSEWIPQASGFETQSRGIFYLHAVDSLVAWAVGYDGSGGSATTNDFTKTVDGG
                     TTWVPGDILGGSTYGIGNICALNGDTAFAAVYNGAGNQDNNCGIYKTMDGGSTWTHLT
                     GALQGSASFADNVYFWNENEGMCHGDTRDNYFEIYTTSDGGATWTRVPKANIGGGANP
                     ASGEGGWTSVIEATGDSTIMFGSNKAKLYISHDRGYTWTISATGITPSTNGGINVIAF
                     KDKMHGLVAQTATTVVVKETSDGGATWQTVTPTGPFFTNDISFVPGTENTYVSTGAAE
                     GAYGASFSEDGGHSWTLFEGTDQSQFLACDFASNSCGWAGGFNTSTTEGGMFKYVGIL
                     TPAGTVLATVTNVTALVYGNIVSLSWTAPSGTGNLLNYNIYRNDTLIGTAPSGQTEYE
                     DLNVANGPQNYCVTAVYDLGKSEPVCTIAWIVVSTEEPAGNALRVYPNPATGYIMVES
                     PENISQVKIMNLVGKEIYRNKVAGNSLKIATGDFKPGMYILQVTGNGKISTRKVTIR"
     gene            4189..6615
                     /locus_tag="PKI35_09520"
     CDS             4189..6615
                     /locus_tag="PKI35_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44662.1"
                     /translation="MIKARKFLVPSSGFGDAYRDSLSVGLSLLITAITFPSFSLITGT
                     GIDPPLSWIFNYFADGNWQAGKDIIFPHGPLAFLMYPLPGNLFQAVVFYSFIRFIFSW
                     LIFKLFFFTGHKGFIIPAVTSFILLGLSDILLVMIGIVMIAGQMYFFERKFRWLFLAF
                     AVTVTAVYIKAFAGIVCLSVTLAVIFILVAEILLKREEPVVLVYPLLFLLMLPLPWIA
                     IYHTPDGFIRFINGNILLAGDNSAAAAMFPYNNWFFLGGGLVLMAVLTGLHLGDIRMR
                     YFLFSAAPAVFAVWRYGIARQDYLHVGTWFLFVVMIAAIFVFLVNRKKLVSCIILAAG
                     LVLFYLNSRNAYYYESYPFSVKGITFLNKAVFNYDKLADSCRVVSDTAIRHNRLDALT
                     REDIGGASVDVYPWDYSFIPANGLNWQPRPVLQSYASYVPGLDRLNELHFRSEKAPAF
                     LIWELDKTTNDLFDGDMESIDGRYLLNDEPHTLIALMERYDFAGIRKAERPVMLLKKR
                     QTPAKARIRIDKPEQTGWERWLDIPGMKGDLLLARIGMHRSLAGDFRSYIYKDQPYYV
                     YYQLDNGEVRFYRMVPANAADGIWINPLIMNPGMVSGSSGVKRIMFKCPDKRLVEPEI
                     KVEWEHVSFSDTPFVLKSLPENKIADSSHLYVLESFFDAETMPAGWKVSGNDDKSPLC
                     HSGKQAVTVLPGSYSPVFEINTSDISVDDSDSMLVIRAAVWTNTQARVEADLVISVDA
                     DGKPLLWKGVPVDNFQIGRRTWNYVYNFARIDKTMLKTKDAVLKVYLWNTGNGPFLAD
                     DMMVRIGKLK"
     gene            6695..8029
                     /gene="ffh"
                     /locus_tag="PKI35_09525"
     CDS             6695..8029
                     /gene="ffh"
                     /locus_tag="PKI35_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNX44663.1"
                     /translation="MFESLSDRLDRAFKLLKGQGYITEINVAETLKDVRKALLDADVS
                     YKVAKEFTDQVKVKAMGQNVLTSVSPGQLMVKIVHDELASLMGGEKVDLNLKGNPSVI
                     LMAGLQGSGKTTFSAKLAHFLHTKRGKSPMLVACDIYRPAAIEQLKVLGEQIGVEVYS
                     EPDTKDPVKIAKNALNHARDHGFNVMIIDTAGRLAIDEQMMNEIAAVKSAVNPHETLF
                     VVDSMTGQDAVNTAKAFNDRLDYEGVILTKLDGDTRGGAALTVRAVVNKPIKFVGIGE
                     KMDAIDVFYPDRMADRILGMGDIVSLVERAQEQYDEEEARKLQKKLAKDQFNFNDFLS
                     QIKQIKKMGNVKDLMGMIPGMGKALKDVEIDDNAFKGIEAVIHSMTPQERESPAIING
                     SRRKRIARGAGVDIQEVNRLIKQFDDTRKMMKTITTGGARQMAQRMKAKGKR"
     gene            8730..9605
                     /locus_tag="PKI35_09530"
     CDS             8730..9605
                     /locus_tag="PKI35_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HNX44664.1"
                     /translation="MQIIDGKKLAIEIKQELARQTAKIIDSGRRAPHLAAILVGDDPA
                     SDSYVKGKEKACREIGFTSSVYRLAADTTVQKLMEIVDFLNNDDDVDGFIVQLPMPGH
                     IPVDKVISRIKPEKDVDGFHPVNIGRMVLGSPAFISATPLGIITMLDRYGIETEGKHC
                     VVLGRSNIVGTPISILLSRKAKPGNCTVTLCHTKTVNIKEIAATADILISAIGKPEYV
                     TADMVKEGAVVIDVGIHRIPDPAKEKGYHMTGDVKYDEVAPKCSYITPVPGGVGLMTV
                     VSLLMNTMKAYNKEI"
     gene            9712..10293
                     /locus_tag="PKI35_09535"
     CDS             9712..10293
                     /locus_tag="PKI35_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNX44665.1"
                     /translation="MEDFYTIQGEGYHSGKAAYFLRIGGCDVGCYWCDVKESWNPKIH
                     PPTPVEEIVKRTLDNPSKTVVLTGGEPLLYNLDLLCGMLHRHGQKVHLETSGSHPLSG
                     SFDWICLSPKKKSPPLQEICSVADELKVIISDETDFEWAEKNARLVRPGCFLCLQPEW
                     SRISVMMPPIVDYILQHPKWNVSLQTHKYMHIP"
     gene            10400..12313
                     /locus_tag="PKI35_09540"
     CDS             10400..12313
                     /locus_tag="PKI35_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX44666.1"
                     /translation="MMKRFLPYFTCILLCFLPLWLLAQHSSNSKAIRLYDKALQLYKQ
                     REYLPTMKQLDEVLKADSLFAEAYLLKGDLFSDLKQPEKAIKEYNNALAIDTAVFPPA
                     YYIIANLQFGMEQYAEARKNYFKYLAFHPKNQVELEKVSRNLPLCEFRMKLMANPVSF
                     NPVNLGEAVNSFGYEYINSVSVDEEQMYFTRRGADRRDNESFFESHKENGQWCPATEL
                     GEPINTSGNEGALCLSPDRMSFYLTCCSRFDSYGSCDLYTSQRYGNQWTEPMNLGKTV
                     NSTAWDSQPCLASDGRTLYFVSSRKGTKGGSDIFCTVKQADGTWSQPVNLGDSINTPA
                     DEMAPFIHADGRTLYFSSEGHPGMGGADLFVSRMDINGNWTKAVNIGYPVNTKGDEIN
                     LVINAGGDAAYISSDKEGGFGNVDIYRFELPKEARPQPVSYVEGRVFDKKDRHPLGAS
                     FELIDLATSQIIVRSTSDAATGEFLLCLPVDHDFALNVTCQGYLFYSKNFSLSDKSGK
                     ESPKQLDIPMQPVSIGETVILHNIFFETDKFELKPESKAELDKLVEFMLNNTSMTIEI
                     SGHTDNTGAEQHNLDLSEQRALAVYNYLVANGIAKERLSYKGYGKSKPIDINDTPEGR
                     ANNRRTEFSVVSM"
     gene            complement(12376..>13306)
                     /locus_tag="PKI35_09545"
     CDS             complement(12376..>13306)
                     /locus_tag="PKI35_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44667.1"
                     /translation="SIFSANPASTDIDSSSRLNVSFSSITCYNKGFYADLCGSTHFKQ
                     SRYGMTGYIRMFDYETGCHLFRPLSTKIDITSTYKINRGRNATFIKPSIGVGVTFLSL
                     VALYSTTEPIYKVNEEDCRFRYSASIAIFNNKINAGLSYIGINRPDYKASEATSWLPA
                     DRKFGISFSNLFIHIALKKKFLNSETGLFIQSGDRFPRIYSIEEHYVSYRRLGCSYSK
                     TKFLLSASFGLLNDNFRFKEYKADILYRLRKFDFGIKFIYCDEPANYNDFVFHSVIQP
                     FPLTNMTMISYSLTYHFNNKRSHYSNWANILVF"
BASE COUNT         3589 a         2961 c         3177 g         3579 t
ORIGIN      
        1 agaagtgaac gaccggaact gttgctgctc ccatttatta aggttagtta ttaaattgat
       61 tcttttcttt tactcatata ttagattaga tgaatgatcc ggaatctatt ctatggaatt
      121 ggttggtaca gtgcatcatt tttttggatt tggatacata ataaattccc aattcttgtc
      181 ttctatagtg ccataatttt caggtagaaa actaaaaaaa tactctttac ttgtgaaaaa
      241 accatatttt ctgttgtatg tcagaaatac aattaggggt ctatggtcaa ccattctctt
      301 ggatgaatca tataatttct ttaaaatttc gtttttttcg tttttgtgaa aaatctgtat
      361 accggaaatg ttatcgagta tttcgttcaa tgagatatta tttaatttaa ttaataaata
      421 actttttcct tctttagata tatactggaa atcagcatta aatacctcat aatctattat
      481 tactctttta aattcaataa tgatagtatc atcgcttacc ttgttcaaac tcattataac
      541 aatcatttct gaagtgtcat ttggtctatt tctaaattta tttacgttct caatatactc
      601 atttacctca ctagtaagtt catgataatc aattttggtt tgcgcaaaag caaacgataa
      661 gatgataagg ttattaataa tgattagaat cagctttttc atggcattaa tgtatttttc
      721 gggtttaccg gcgggcacta aattacttaa ttgtttgctg aaaaggagcc ttgtttttta
      781 gctaagtacg tatgcccgga acagatttgc cagaggcagg cctttagccc agtcaaaatt
      841 ccgggatgaa cccaaaaaat ggcgggccat atatccttaa agattctgca gtccataaaa
      901 aagaaaaagg ctgccttttc tggcagcctt catcctgatc aaagatttaa gtcgctaacg
      961 ataagttatt taaactatcc ggaggcaggc gttatctttg aacctaaatc atattaccgg
     1021 atcgtaacct tccttgtcga gatttttcca ttgccggtga cctgtaaaat gtacattccc
     1081 ggtttgaaat ccccggttgc gattttaagg ctgttgccgg ccaccttgtt ccggtagatt
     1141 tctttaccca ccaggttcat gatttttacc tggctgatat tctccggcga ctctaccata
     1201 atatatcccg tagctgggtt gggataaacg cgaagagcat tgcctgccgg ttcttccgtg
     1261 cttacaacga tccatgcaat ggtgcaaacg ggttccgatt tgccgagatc gtaaacggct
     1321 gtaacgcaat agttctgcgg gccattggca acattcaggt cttcatattc tgtctggcct
     1381 gaaggtgctg tcccgatcag ggtatcgttc cggtaaatat tataattcaa caggttgccg
     1441 gtacccgaag gggctgtcca cgaaaggctg acgatgtttc cgtaaaccag ggctgttaca
     1501 ttagtgactg tagcaagaac ggtaccagca ggggtcagta tgccaacata tttgaacatg
     1561 cctccttcgg tggtgctggt attaaatcct cctgcccatc cgcaactatt gctggcaaaa
     1621 tcgcaggcaa ggaattgtga ctgatcagtg ccttcgaata gtgtccacga gtgaccgcca
     1681 tcttcgctga atgaagcgcc gtaagcgcct tcggcggctc cggtcgaaac ataagtattt
     1741 tcggttccgg gaacaaagct gatgtcattg gtgaagaaag gccccgttgg tgtgacggtc
     1801 tgccaggtag ccccgccgtc ggaggtctcc ttcacgacaa ccgtggtagc ggtttgggcc
     1861 acgagcccgt gcattttatc cttgaatgca atgacattga tcccgccgtt ggtgctgggt
     1921 gttataccgg tagccgaaat ggtccaggta tagccgcggt catgcgagat atacagtttg
     1981 gccttgttcg atccgaacat aatggtgctg tcgcctgtgg cttcaatcac ggaagtccag
     2041 ccgccttcac cggaagccgg gttggcgcct cccccgatat ttgctttcgg cacacgcgtc
     2101 caggtggctc ccccgtcact ggttgtgtag atctcgaaat aattatctct tgtatcgcca
     2161 tgacacatgc cttcattttc gttccagaag taaacattat cggcaaaaga tgcggacccc
     2221 tggagagcgc cggtcaggtg agtccaggtc gatcccccgt ccattgtttt ataaataccg
     2281 cagttgttat cctggttgcc tgcgccatta tatactgcgg caaaggctgt atcgccgtta
     2341 agggcgcaaa tatttcctat cccataagtg cttccgccaa gaatgtcacc gggaacccag
     2401 gtagtaccgc cgtccaccgt ttttgtgaag tcgtttgtgg tagcgcttcc tcctgaaccg
     2461 tcatagccaa cagcccatgc aaccaggctg tcaacggcat gaagataaaa gatgcccctc
     2521 gattgtgttt caaagccact ggcttgtgga atccactcgc ttagtccgac ggatacctgg
     2581 tgatagaccg tgtccgtttc gttcaatgcg actactttaa cggtgtgact tccgagtgca
     2641 taggcagccg ttggccatga atagacaaac gatgcctgtg ctgaggtata gacaatatta
     2701 tcgtcaatgt acaggttaac ggttgttgca gttccttttt caactgagca gttaatgtca
     2761 aaagccgatc ggtaaggtac tatctgttga ggtcccatgt ttgtaatacg aacgatgaga
     2821 tcgggattgt aaggttgaat tccgattaca gcatgattat tttgattagg agaataccct
     2881 ggaggattca gggcgccgat agtaaaatac ccgttgccgg aacctgacca tccccagttc
     2941 atgtgaaact tgctatcgct tgacctgtaa ccgtcgcaca cccaggcatg accggatgat
     3001 ccgtcatcgc cggcatagta gataggcctg ctggctgtaa gatcttcaat cagaatgttt
     3061 ttccattctg taagagagta atcggctttg tataccagcg aaatggttga aggatcataa
     3121 ttaaaatagg tgtttaatgc ccatgggacg tctgaacttt gagcgccgga accatcgata
     3181 ccatagttca tatcaacagc tacgccatta tggtaaagta aagtggctat agattggtag
     3241 ttactgcttg tggcctgggt tggcatctgc gcccaattat aggtcgtagc tccaaagttt
     3301 gctgtctgtt gtccataagt ggggtgagta taagaatgag agaggacacc ctgttccggg
     3361 aagttatgaa atttcataat ttgtcccatg gcagttgcaa cgcaacctgc ccaaacatgg
     3421 cctccctggc ctcctgatgc agccgggcag taataattat accaagcgcc ctgatcccac
     3481 gaagtggtaa caagaggatc aacatcgttg gtgccacggt caaaattatt attcaacaac
     3541 ttattccatg aaccgatggt atattcgttg ctaaggcgtg aatctactgc gccttttatt
     3601 tcctgattat atccatcaaa ccaatactta aggtttggct gtgagaattc cgcagggaaa
     3661 tttccggtat tggactgtgc cagcacggga acagcagcgt cgtcagccga cacaaccaca
     3721 aagccgcctc cttcataatt gaatatgaaa cacgttatag tgcctttata ttcgatgacg
     3781 gaactttctt tgagcgtgag ttcggatttg cctgaataat gcctgaaata attgtcggca
     3841 acaagacggg ccttttcgat agtaaccgga tttgcagttg caaataccac aagcaataat
     3901 gagattgcca gtgtagatag ttttttcatt ttcagaggat tgatttgacg gagatgattt
     3961 tttcgggtga aattttggga aaaattgatg gttgttttca ttacaaaaat agcattattt
     4021 ggatagtaaa gcctgacggg cagattttta tgtccggaag gagagttgcc cgattgtttc
     4081 tgatatttcc cggacggagg atatttatgt ctttttgccg ccggaatgag tatgagcatc
     4141 ggcgcgtatc gactaacctt ataaattcgt gcggaaattc gcgttcagat gattaaagcc
     4201 cgaaaattcc ttgtgccttc ttccggattt ggtgacgcat acagggatag cttgtcggta
     4261 ggtttatcgc tgctgattac agccattact tttccttcgt tttcattgat tacgggaaca
     4321 ggcatcgatc cgcctttgtc ctggatcttt aattactttg ccgacggaaa ctggcaggcg
     4381 gggaaggata tcatctttcc gcacggcccc ctggcatttc tcatgtatcc tttgcccggc
     4441 aatctttttc aggctgttgt cttttattct tttatccgtt tcatcttctc ctggctcatt
     4501 tttaaactct ttttctttac cggacataaa ggattcatca ttcccgcagt aacatctttt
     4561 attttgctgg gattgtccga tatattgctg gtcatgatag ggattgtaat gatcgccggc
     4621 cagatgtatt tcttcgaaag gaagttcagg tggctgtttc ttgcttttgc cgtaacggtg
     4681 acagccgttt atatcaaagc ttttgcggga atcgtctgcc tgtcggtaac acttgccgtg
     4741 atcttcattc ttgttgccga aatactgctg aaacgggaag aacccgttgt gctggtttac
     4801 cctctgttgt ttcttttgat gctgcctctt ccctggatag cgatctacca taccccggat
     4861 ggttttatcc ggtttattaa cggcaatatt ttgctggcag gcgacaactc ggcagcggcc
     4921 gcgatgttcc cttataacaa ctggtttttc ctgggcggcg gtcttgtttt aatggccgta
     4981 ttgacaggcc tgcacctggg cgatatccgc atgcgctatt tcctgttttc ggcagcgccg
     5041 gccgtatttg ccgtgtggcg atacggcatc gcacgtcagg attacctcca tgtaggaaca
     5101 tggtttttat tcgttgtgat gatagctgct atattcgtat tcctggtcaa ccggaaaaaa
     5161 ctggtttcct gcataattct ggccgccgga ttggtgcttt tttatctgaa ttcccggaat
     5221 gcttactatt atgaatccta tccgttttcg gtcaaaggaa ttacttttct gaacaaagcg
     5281 gtttttaatt atgataagct ggctgattct tgcagggtag tttccgatac ggcgatacgg
     5341 cacaacaggc tggatgcttt gacgcgggaa gatatcgggg gcgcttcggt ggacgtatat
     5401 ccctgggatt acagttttat cccggccaac ggactgaact ggcagccgag gcctgtactc
     5461 cagagttacg cgtcgtatgt tcccggtctt gatcggttga atgaacttca tttcagatcg
     5521 gagaaggcgc cggcattcct catctgggaa ctggataaga ctaccaatga cctgtttgac
     5581 ggcgatatgg aaagcattga tggacggtac ctcctgaacg atgaaccgca tacgctgata
     5641 gccctgatgg aacgatatga ttttgcagga atcaggaagg ccgaacgtcc ggtcatgctt
     5701 cttaaaaaga ggcaaacacc tgccaaagcc cgcatccgga ttgataaacc ggaacagacc
     5761 ggttgggaaa ggtggctgga tattcccggg atgaaaggcg atctgctgct tgccaggatc
     5821 ggaatgcacc gaagccttgc cggtgatttc cgaagttata tttacaagga tcagccgtat
     5881 tatgtatatt atcagctcga taacggcgag gtcaggttct accggatggt tcccgccaat
     5941 gccgccgacg gtatctggat taacccgttg atcatgaatc cggggatggt ttccgggagt
     6001 tcaggggtta agcgtattat gtttaaatgt ccggacaaac ggcttgttga accggaaata
     6061 aaggtggaat gggaacatgt ctctttcagc gatacccctt ttgtattaaa atcgctgccg
     6121 gaaaataaaa ttgcggattc ttcgcactta tatgtacttg aatcattttt cgatgcggaa
     6181 acaatgccgg cagggtggaa agtatccgga aatgacgaca aatctccgct gtgtcattca
     6241 gggaaacagg ctgtaaccgt gttgccgggg tcctattcgc cggtctttga gataaatacg
     6301 tcggacattt ctgttgatga tagcgacagt atgctggtta tcagggctgc cgtctggacg
     6361 aatacacagg cgcgtgtcga agccgacctt gtgatatcgg tggacgctga cggcaagccg
     6421 ttgttgtgga aaggagtccc ggttgataac ttccagattg gccgtcggac ctggaactat
     6481 gtttataatt ttgcccggat cgataaaacg atgttaaaga caaaagacgc tgtgctgaag
     6541 gtttacctgt ggaatacggg aaatgggcct tttcttgccg acgatatgat ggtgcggatc
     6601 ggaaaactta aatagggccc atacagcctg tttcgccggt tagcggataa atcgtacttt
     6661 tgtatcctga atcatagaac gaattatttt tacgatgttt gaaagtttaa gcgacaggct
     6721 cgacagggcc tttaaactcc tcaagggaca gggatatatt accgaaatca atgttgccga
     6781 aacgctgaag gatgttcgca aggcgctcct cgatgccgac gtaagctata aggtagccaa
     6841 ggagtttacc gaccaggtga aagtcaaagc catggggcag aatgtgctga cttcggtatc
     6901 gccgggacag ttgatggtga agattgtcca cgacgaactg gcatcgctga tgggcggcga
     6961 gaaagtcgac ctgaacctta aaggcaatcc ctcggtgatc ctgatggcag gcttacaggg
     7021 ctccggtaag actaccttct cggcaaaact ggctcacttc ctgcatacca aacgcggtaa
     7081 aagcccgatg ctcgtggcct gcgatatata ccgccctgca gctatcgaac agttaaaggt
     7141 gctgggtgaa cagataggcg tcgaagtata ttccgaaccc gataccaaag atccggtcaa
     7201 aatagcgaag aatgccctga accatgcccg tgatcatggt ttcaatgtaa tgatcatcga
     7261 tacggccgga cgtctggcta ttgatgaaca gatgatgaat gaaatagcag cggtgaaatc
     7321 ggcggtgaat cctcatgaaa cccttttcgt tgtcgattca atgacaggtc aggatgccgt
     7381 gaacacggca aaagctttca acgatcgtct ggattatgaa ggcgttatcc ttaccaaact
     7441 cgatggagat acccggggcg gcgccgcttt gacagtccgc gccgtagtga acaagcccat
     7501 caagtttgtg ggtatcggcg aaaagatgga tgccatcgat gtcttttacc ccgatcgtat
     7561 ggccgaccgc atcctgggaa tgggtgacat tgtgtcgctg gttgaacgcg cacaggagca
     7621 gtatgatgag gaagaagcaa ggaaactcca gaagaaactg gctaaagacc agtttaattt
     7681 caatgatttc ctttcccaga tcaagcagat taagaagatg gggaatgtga aggacctgat
     7741 gggaatgatt cccggcatgg gcaaggcatt gaaggatgtt gagattgatg acaatgcttt
     7801 taagggtatc gaagccgtca tccattccat gacgccgcag gagcgtgaga gtccggccat
     7861 catcaacggt agccggcgta aacgtattgc ccgtggcgcc ggcgtcgata tacaggaagt
     7921 aaacaggctg ataaagcagt ttgacgatac ccggaaaatg atgaaaacaa ttacaacagg
     7981 aggggcgcga cagatggcgc aaaggatgaa ggccaaaggg aaaaggtaaa tggcgaggag
     8041 cgaggagaac ggagaacgga gaactgctct gacaaaggct taaatccaaa ccctaagact
     8101 ctgaaaccct gccgcctccc acagcccagc gcaaggcagg caggcctgcc acctgacgca
     8161 cagccttccc gggcaggcag gcctgaaaca ctgaaagccc acgaacaacg aatcacaaaa
     8221 cacaaaacac aaaacacaaa acgcaaaaca caaaacggag aacgcagaac ggagaacgca
     8281 gaacggagaa cggagaactg ctctgacaaa ggcttaaatc caaaccctaa gactctgaaa
     8341 ccctgccgcc tcccacagcc cagcgcaagg caggcaggcc tgccacctga cgcacagcct
     8401 tcccaggcag gaatgtctga aactctgaaa ccttgaaacc ctgccgcctc actcacaatc
     8461 ctctgcaggc aggcaggtct gaaacgctga aacgctgaaa ccctcatcta ccatcttctg
     8521 ctgcaaaggc aaaacgcaaa acgctgaaca caaacgcaaa acacagaact tagaactcaa
     8581 aacgctaaca gtttacctgc ccgggcagga ttgtgcatga gttggcaggc taaacgccaa
     8641 cggctaaaag ctaatggtga acaaccgata gctatcagcc agatgaaaac gaaaaccaga
     8701 aacttcaaac tttaaactca ggaatcaaga tgcaaattat tgacggaaag aaactagcca
     8761 tcgagataaa acaggaactc gccaggcaaa ctgctaaaat cattgattcg ggcaggagag
     8821 ccccgcatct tgcggctatc cttgtcggcg atgatccggc cagcgattca tacgttaaag
     8881 gcaaagagaa ggcatgtcgg gagattgggt tcacctcttc ggtatacagg ctggcagccg
     8941 atactacggt acaaaagttg atggaaattg tcgatttcct gaacaacgac gacgatgtag
     9001 acgggtttat agttcaactt ccaatgccgg ggcatattcc tgtggataaa gtgataagcc
     9061 gcattaagcc ggaaaaggat gttgacggtt ttcacccggt aaatatcggg cggatggttc
     9121 tgggcagccc tgcttttatt tctgcaacgc cgttggggat tatcacgatg ctcgaccgtt
     9181 acggcatcga aacagaagga aaacattgcg ttgtactggg acgcagcaat atcgtcggta
     9241 cgccgatcag tatcctgctt tcgcgaaaag caaagccggg aaactgcact gtgacgcttt
     9301 gccataccaa aacagtcaat attaaagaaa tagcggccac cgccgatatc ctgatctcgg
     9361 caatcggaaa gccggaatat gttacggctg atatggtgaa agaaggcgct gtagtaattg
     9421 atgtgggtat tcaccgtatt cctgatcctg ccaaagagaa gggataccat atgaccggcg
     9481 atgttaaata cgatgaggta gccccgaaat gcagctacat aactcccgta ccgggtggtg
     9541 ttggccttat gacggtcgtt tcactgctga tgaacacgat gaaagcttat aacaaggaaa
     9601 tctgaacctg accgggggct gaacagaata gccatttccg cggggaaaat cattaattaa
     9661 aaacatactg aggtgaatac caacaattcc ggtggacaat tgcttcccct gatggaggat
     9721 ttttatacca ttcagggtga aggatatcat tccggcaagg cagcctattt tttaaggata
     9781 ggcggttgcg atgtgggttg ttactggtgt gatgtgaagg aatcgtggaa tcctaaaatt
     9841 catcctccca ctccggtgga agaaatcgtg aaacgcacgc tggacaatcc ttcaaaaacg
     9901 gtagtgctta ccggcggcga accgctttta tacaacctcg acctgctttg cggaatgttg
     9961 cacagacatg gtcagaaagt tcatcttgaa acatcgggtt cgcacccttt gagcgggtca
    10021 tttgactgga tctgcctgtc acccaaaaag aaaagtcccc cgttgcagga aatctgttcc
    10081 gtggccgacg aactgaaagt gatcatctct gatgaaacgg attttgaatg ggccgaaaaa
    10141 aatgcccgtc tcgtcagacc ggggtgtttc ttgtgcctgc agcccgaatg gagccgcatc
    10201 tccgttatga tgcctccgat tgtggactat atcctgcaac acccaaagtg gaacgtttca
    10261 ctgcaaaccc acaaatacat gcacattccg taagtcggaa tttccgtaat attttcagga
    10321 attataatat tcaccttgac aatccgttgt aatgttggtg aatgtcggat tattatttat
    10381 cttcgcttat atttaattca tgatgaaacg ctttctccca tattttacct gcattttact
    10441 gtgttttctg ccattatggt tattggccca gcattcctcg aattcaaagg ctattcgctt
    10501 gtatgataag gctttacaac tgtataaaca acgcgagtac ctgcctacca tgaaacagtt
    10561 ggatgaagtg ctcaaagctg attccttatt tgctgaagct tatcttctga aaggcgacct
    10621 gttcagcgac ctgaaacaac ccgaaaaagc catcaaagaa tataataatg ctcttgctat
    10681 tgatacggca gtctttccgc cggcctatta tattattgcc aatcttcagt ttggaatgga
    10741 gcaatatgcc gaggcgagga agaattattt taagtacctg gcatttcacc ccaaaaatca
    10801 ggttgaactt gaaaaggtat cgcggaacct gccgttatgt gagttcagga tgaaactgat
    10861 ggcaaatccg gtttctttca atcccgtgaa cctcggggaa gctgtgaatt cattcgggta
    10921 tgaatacatc aattccgtga gcgttgacga ggaacagatg tattttaccc ggcgtggcgc
    10981 cgaccggcgt gataacgaaa gcttctttga atcgcataag gaaaacggcc aatggtgccc
    11041 cgccacggag ttgggagaac ctatcaatac cagcggtaac gaaggtgcgc tctgcctttc
    11101 acctgaccgg atgtcgtttt acctgacctg ttgcagccgt ttcgattcct atggctcctg
    11161 cgatctttac acttcgcagc gttatggtaa ccagtggaca gaaccaatga atcttgggaa
    11221 aaccgtgaat tcaactgcct gggactcgca accatgcctg gcgtcggacg ggcggaccct
    11281 ttattttgtc agcagccgca agggtacaaa aggaggttct gatattttct gcaccgtaaa
    11341 acaggccgac ggaacctgga gccagcctgt aaacctgggc gattccatca atacaccggc
    11401 cgatgaaatg gcgccgttta ttcatgctga cgggcggacc ctgtattttt cttccgaagg
    11461 ccatcccggc atgggcggcg ccgacctctt tgtttcgcgc atggacatta acggcaactg
    11521 gactaaagcc gtgaatatcg ggtatccggt aaatacgaaa ggcgacgaga tcaacctggt
    11581 gatcaatgca ggcggggatg ccgcctacat ttcaagcgac aaggaaggcg gcttcggcaa
    11641 tgtggatatc tatcggtttg agttaccgaa ggaagcgcgt ccgcaaccgg tttcctatgt
    11701 tgaaggacgg gttttcgata aaaaagatcg gcatccgctt ggcgcaagtt ttgaattgat
    11761 agaccttgct accagccaga tcattgttcg atcaacctcc gatgcagcca ccggggagtt
    11821 cctgctttgt cttcccgttg atcacgattt tgccctgaat gttacctgcc agggatatct
    11881 gttctactct aaaaatttca gcctttccga taagtcagga aaagaaagcc ctaagcaact
    11941 cgatatcccc atgcagccgg taagcattgg cgaaacggtg atcctgcata atattttctt
    12001 cgaaacagat aagttcgaac taaagccgga atcgaaagcc gaacttgata aactggtgga
    12061 attcatgctg aacaatacca gcatgacgat tgaaatcagc gggcataccg acaataccgg
    12121 cgccgaacag cacaacctgg acctttcgga acaaagggcg ctggcggtat ataactacct
    12181 ggtggctaat ggcattgcaa aagaacggct atcctataaa ggttatggta aaagcaaacc
    12241 catcgatatc aacgacacgc ccgaaggccg cgccaataac cgccggactg aattcagcgt
    12301 tgtttcgatg tgaccatgtc attcacatct tatgccattc ctgtctttaa agttgcgact
    12361 caaataagtt gcgactcaaa acacaaggat gtttgcccag tttgaataat gggaacgttt
    12421 gttattgaaa tgataagtaa gagagtagct tatcatcgtc atatttgtta aagggaaagg
    12481 ctggattacg gaatggaaaa caaagtcatt gtagttagcc ggttcatcgc aatagatgaa
    12541 tttgattccg aaatcaaatt ttcttaatct atacaaaata tcggctttgt attccttgaa
    12601 tcgaaaatta tcattaagca atccaaacga tgctgagagc agaaatttag tttttgaata
    12661 cgaacaccct aatcgtcggt aggaaacata atgttcttct attgaataaa tacggggaaa
    12721 tctatccccc gattggataa ataaccctgt ttcagaattc aagaatttct ttttcagtgc
    12781 tatatgaata aaaaggttcg aaaaagaaat accgaatttt ctatctgctg ggagccatga
    12841 tgtggcttca gaagccttgt agtcaggtct gtttattcca atataactta atccggcatt
    12901 aattttatta ttgaatattg ctatagatgc ggaataacga aaccggcaat cttcttcatt
    12961 gactttatat attggctcag ttgttgaata aagtgctacc aaacttaaaa aagttactcc
    13021 tactcctatt gaaggtttga taaaggttgc attcctccct ctattaattt tataagtgct
    13081 ggttatatca attttggttg ataatggcct gaacagatga cagcctgttt cataatcaaa
    13141 cattcgtata taacctgtca tgccatatct tgactgtttg aaatgtgttg aaccacataa
    13201 atcagcataa aaacctttgt tatagcaagt tatgcttgaa aatgatacat ttagtctgga
    13261 agaagaatca atatctgtag aagctggatt agctgagaat atggag
//
LOCUS       DAOPYV010000074        12814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_5418_length_12814_cov_11.416020, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000074 DAOPYV010000000
VERSION     DAOPYV010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12814
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5418_length_12814_cov_11.416020"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PKI35_09550"
     CDS             complement(<1..941)
                     /locus_tag="PKI35_09550"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNX44668.1"
                     /translation="MPTKPIDPKILERANRWLQGNIDDETREQIKYLIENDPDELTES
                     FYCDLEFGTGGLRGIMGPGTNRMNRYTVGMATQGLANYLKSIYPNEEELRVAIAYDSR
                     NNSELFAKVSSLVLSANGIHVFLFEGMRPTPELSFAVRYLHCHSGIVITASHNPRDYN
                     GYKVYWNDGAQLIPPHDKNVIAEVQKITSIDDVKFSGDSTIIDPIGEFIDTAYLQAIM
                     KYSLAPELIEKHKDLNIVYTPLHGTGITLVPPALTKFGFENVNIVKEQAVASGNFPTI
                     YSPNPEEKSAMEMALLLAEKVGAELILATDPDADRIGV"
     gene            complement(1115..1987)
                     /gene="rfbD"
                     /locus_tag="PKI35_09555"
     CDS             complement(1115..1987)
                     /gene="rfbD"
                     /locus_tag="PKI35_09555"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNX44669.1"
                     /translation="MHILITGSNGQLGSEFRLLAPQYPDHDFIFTDVAELDITDADAV
                     KNFLSDNDVDVIINCAAYTAVEKAETERTLAYRINVEGPENLANQAFKRNVLLVHIST
                     DYVFEGTHSKPASELDPVKPIGFYGATKLFGEKAIKNSGARALIIRTSWLYSAFGVNF
                     VKTMRRFGLERGELRIVDDQIGSPTYAVDLARTILRIMPEYKRHDCDIFHYSNEGVAS
                     WYDFACEIIRISNIACTVSPISTDEYPSQAPRPAYSVMSKQKIRKTFGLEIPYWRDSL
                     KDCIERLETPEKNR"
     gene            complement(1999..3594)
                     /locus_tag="PKI35_09560"
     CDS             complement(1999..3594)
                     /locus_tag="PKI35_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44670.1"
                     /translation="MKMNYLFFVLVLFSCMAGAKGETYIVMNGSDSGDLSLRHFIEIS
                     GPYDTILFSADVDTVTLTSDELKIEQPLCILGNENKTVIRRDPAAGTPDFRIFRLVNS
                     GVVYFKNLVICNGKSICIGGVYPQPGGGIQINDTLSYLYIDHCILHSNLAASGPDLGY
                     SASYPAPPGASGGAIANYGHLEIKNSDIYNNSAGTGGRKTIVKSDNSMYDCFPSNGGS
                     GGGIWSGHYLKIENCKLTGNSSGYGGFCPDPLNPGSGWWAAGGSGGALCIISGSSTSI
                     INSIISGNKVRYGTPAGSGGGILSAGNLDILSSTITRNYSMSPNETGGGMKLNNAVTS
                     VRNSIIFGNYLQVSTNLSDVSVYDGSYIFSHSLVGKPVGFEITDTTNLIGEDPSFISI
                     DDLRLAFNSPCINTGDPDTTGLPPIDLAGMPKVVENRVDMGAYEFQGYSWDHKNSANP
                     EIFIYPNPCTDKITIFIPEYLKNSVLEVHLIDVAGQVLSGYSIAKGSSSFCISLPASL
                     PKGIYYLEAHTSDHISAHKIIKL"
     gene            complement(3955..4413)
                     /locus_tag="PKI35_09565"
     CDS             complement(3955..4413)
                     /locus_tag="PKI35_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44671.1"
                     /translation="MEETTAPEVTSPATPPMTPEALIHLQIAAKWAKFLSIVGFVLTG
                     FMILTGLSFAFIMKPLETQIPVFAQIPAILVSVVYIIFAAVFLLPVLFLNSFATNVPK
                     AIRKNDSQFLTKACKNLKSLFAVIGLITILMLIIYLFALLLIGGAALLAF"
     gene            complement(4436..6610)
                     /locus_tag="PKI35_09570"
     CDS             complement(4436..6610)
                     /locus_tag="PKI35_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HNX44672.1"
                     /translation="MKYKNLFFFLVIAFSFTTPAFSQQAKYSLKDEFLKINAEAGDPL
                     YTTYAAGINRSRMYGDKAYKMDYYSPCEPLNFSSDQAGRIYSTWRINSVVIDRMNKYH
                     RLPVVTASFPDMALMEYEPWEGLEVQQLFFVYSSSIAIVDMSITNNSDKPLTVDLYPT
                     LELGNDSLFIDRWDAENSAYILTHRESLTRLISSLNPGFPYPTSCRDIFTAGFEPYST
                     GGYFGNLDDMYNFVKRDFYAEKNTHDSLNCIQGGYTDFVMLHNRFSLDPGQQKHIRYF
                     RGCCDISESETTLMAEIQALKNIPLQPFIDENERLFAAVPRLKFNNASDKLVYLGAFN
                     LVRGCMLPPAGKTSHNFYVFSRNPLWGWGHGHQVLHESLSMLSYACLDAASAEESQRV
                     YMEQQGADGLIAYRHGPRGMQDYPHKGMPTTSAPFYNWINWEIYTVSKDKSFLRDAYE
                     SGVKYTNWLIRNRDTDHDGTFEWGPYGIIENVRDWYNAVFQVSAERYLDVDKEDISDE
                     LECLDLTLMVVKEMRSLSVMAGELGREKDSREWSAKADTVSRLVNERMWDSETGFYYS
                     VNKNDHSFRFMTRDLRRQEIIAFLALWAEAAPGDRAEILLKKLTDPGKFWRKYGVPTL
                     AADDPWYTPYVDYCCKWNGPVWLLWNYMVFDGLRNYGYDDAARELAGKMLLCVKTQLA
                     ANHNYWESYSPDNDVLDSPSNYIWDSIMAKLMIDLDRMNQPK"
     gene            complement(6765..8813)
                     /locus_tag="PKI35_09575"
     CDS             complement(6765..8813)
                     /locus_tag="PKI35_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX44673.1"
                     /translation="MKKLFLLAFTTLLIAQSVMAQPTERARRNMNLFNYSKAIKILNH
                     AVKKEKYRETAIPLLAECYKMQNDPANARGWYSQAVQIPNAGADTWFEYARSLMQAGE
                     YTQANQALDKAAALAPQDPRIKRYTAYCDSVQVTWKDMAPAFEIKTLSNVNSEASDFG
                     PAFYANGFTYASDRERNVLDERPYGWTGRGYVKLLFTKPEKTGDVFGNIRYPRSLKGF
                     FNETYHDGPAYFAGDSVVAFTRSYHDADAKKINNVRTDMLKIFYASRVNGKWGKLNKF
                     YLNSPDYNVGHPALTADASVMYFVSDMPGGQGGTDIWMCKRQGEGWGNAVNLGPVINT
                     PGNEMFPSVKQDGTLYFASDGHPGYGGLDIFSSKFSDGSWSVPRNLYAPLNSSYDDFA
                     LAWMPGTSYGMFSSNRPGGEGWDDIYAFRKLPEPPAPIKETEPLPAMITGFVKDKTTL
                     RPMPNATVFVLNEETDEIVILKTDDKGAYSHQLKTPAQLIVKATTKGYIADCMTWPEE
                     HIKAGETTPASRDLLLDKLAESKTFVIENIYYDFDKYNIRPDAEPPLENLVRIMKDNP
                     INVELSSHTDCRGSYAYNDKLSQNRAESAVRYIIAQGIDPSRITAKGYGEYKLTNRCS
                     DGVPCSAAEHQANRRTEFKVISTSTTIPEKDLFNPDQFKAGETINRSALPPDFFMQCK
                     "
     gene            complement(8876..9793)
                     /locus_tag="PKI35_09580"
     CDS             complement(8876..9793)
                     /locus_tag="PKI35_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNX44674.1"
                     /translation="MKKIIPILLLLLASSAFAQQDPLFSQYMFNKLALNPAYAGSREA
                     LSVDVLGRDQWEGIAGAPKTLSASIHSPLRNPHIGLGANVYCDAIGNSVYSGALATFA
                     YRIIFPEGKLSFGIQAGYKHVYFDWSKMYLLNTEDQMFLGQESKKAVPDANFGIYYYT
                     NRYFVGFSSKQLLQNQMAEVIVDGKSEFTKLLRHFYGMAGAAFPVADNIVFRPSILAK
                     FVPNAPPQLDINASVLFANTFWIGASYRTEKALSLMTEINLSQNIRLGYSYDVWFNEL
                     RSQNRGSHEIRLGFDFDVFQSRMHTPRYF"
     gene            complement(9893..>12814)
                     /locus_tag="PKI35_09585"
     CDS             complement(9893..>12814)
                     /locus_tag="PKI35_09585"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNX44675.1"
                     /translation="GNFATTTATFTIQDTTKPAITCPENIIVNNDPGLCSATVTVPVP
                     AVVEACSSVTLVNSFNQSSNASGVYPVGTTTILWIATDECGNADSCSMTVTVVDNEQP
                     VITCPADLVLCDTEPVVLGQATATDNCDPNPVITNNAPAVFPVGVTYVTWIAIDIHNN
                     ADTCIQSVTIIAHPTANAGPDQTICQADNFTVTGASATNYASVLWTHSGQGSLLNANT
                     LTPTYVPQVGETGNIYLTLTAYANTPCADSSDQMLLSILPQPVATAGENSTICSDQTF
                     VPVTAYASNYNSILWTTSGTGTFDNPGVIHPVYTPSQADLAAGSVILTLTAFGSTPCG
                     DISDPMTLTFATAPAANAGPDDISCAAAPYTLSGSAAAGESLLWTSAGTGTFDNATIL
                     HATYTPSTADIAAGSVVLTLHAYGIALCESAADDMTLTILPATSAFAGEGATICNSTP
                     FTIGDATASGYTSILWTHNGQGTLTGETTLTPTYTPAVDETGNVTLTLTVTGTAPCGD
                     ATDEAVLTIQPIVAATAGPDLYTCETAPIAISQASAQNFSAIAWTTSGTGTFDDAALL
                     NPVYTPSAADMQAGTVILTMNVTGLAPCGDIQDNLTLTINLAPVAGAGPDASTCQSIP
                     FTVSGASASNYSSILWSSNGTGTLTGETTLTPTYTPAAGESGTIILTLTVNGPAACGN
                     VVATDQLSLIVYESVIANAGLDQSIPANTATALDGSASGGSGMYTWSWQPESLLSDAS
                     VANPSTLPLAQPTTFTLTVLDLASGCYSSDEVTVSVFLNSNNPPVAIDDYDTTSMEQP
                     VTISILDNDYDPDGDPITVSLLTYPHNGTIILNANQTVTYTPAANFFGIDTCTYVICD
                     NGQPSLCDTATIFITVLGTRENLIFYNGISPNRDGLNDVWIIGNIEFFPENKVIIFNR
                     WGDIVYSVANYDNKNVVWDGTYEKSGNHVPDGTYFYIVTVPGLGKYDGWILVRGSKES
                     E"
BASE COUNT         3003 a         2811 c         3717 g         3283 t
ORIGIN      
        1 acgccgatac ggtcggcatc gggatcggtg gccaggatga gttcggcgcc gactttctcg
       61 gccagcaaca acgccatttc catggccgat ttttcctcgg ggttgggcga gtaaatggtc
      121 gggaaattgc cgctggcaac ggcctgttcc ttgacgatat tcacattttc gaaaccaaat
      181 tttgtcagcg ccggaggaac cagggtgatg ccggtgccgt ggagcggagt ataaacgatg
      241 ttcagatcct tgtgtttttc gatgagttcc ggcgccaggg aatatttcat gatggcctgg
      301 aggtaggcgg tgtcgatgaa ttcaccgatg gggtcgatga tggtactatc gcctgaaaat
      361 ttaacgtcgt cgatggaagt gatcttctgt acttcggcga tcacattttt gtcgtggggc
      421 ggtatcagtt gtgcgccgtc gttccagtaa accttatatc cgttataatc tctcgggtta
      481 tgcgaggctg taattacaat tccgctgtga cagtgaaggt aacgcacggc aaacgacagt
      541 tcgggggtgg ggcgcatgcc ttcgaacagg aaaacatgga tgccgttagc cgagagcacc
      601 agggacgata ctttggcaaa caattcgctg ttgttgcgcg aatcgtaagc tatggcaacg
      661 cgcagttctt cttcattggg ataaatgctt ttaagataat tggccagtcc ctgtgttgcc
      721 attccaactg tatagcggtt catccggttg gtcccggggc ccatgatgcc ccttaatccg
      781 ccggtgccga attcaagatc gcaatagaaa ctttcggtca gttcgtcggg gtcattttca
      841 ataaggtatt ttatctgttc ccgggtttcg tcgtcgatat ttccttgcag ccaccggttg
      901 gcgcgttcaa gtatttttgg gtcaatgggt tttgttggca tcgaaaaatg ttattggtta
      961 acggtttgca gtattccggc tggtgaccgg gggctgactg aacgttactg agtatcgctg
     1021 gtgtaaggtg caaaaataag gaagaattta tcccgactgt tgatgattta ttcagaataa
     1081 atattaaaat cctttttgtg acagatgctg cgatttaccg gttcttttcc ggtgtttcga
     1141 gccgttcgat gcaatctttg aggctgtcgc gccagtaagg gatttcgagg ccgaatgttt
     1201 tgcggatttt ttgcttgctc atgacgctgt aagccggacg gggcgcctgc gacgggtatt
     1261 catccgtaga gatggggctt accgtgcagg cgatatttga gattctgatg atttcgcagg
     1321 cgaaatcgta ccaactggca actccttcgt tcgaataatg gaagatgtca caatcgtgcc
     1381 gtttgtattc aggcatgatc cggaggatgg tcctggcgag atcgacggca taggtagggc
     1441 ttccgatctg gtcgtccaca atgcggagtt ccccgcgttc gaggccgaac cggcgcatgg
     1501 ttttcacaaa gttgacgccg aaggctgaat aaagccacga tgtgcggatg ataagcgccc
     1561 tggcgccgct gtttttaatg gctttttctc cgaagagctt ggtggcgcca taaaaaccga
     1621 taggctttac cggatcaagt tcggaagccg gctttgaatg ggtgccttcg aaaacgtaat
     1681 cggtagagat atgcaccagc agcacgttcc gtttaaaagc ctggttggca agattttcag
     1741 ggccctccac attgatccgg tatgcaagcg tccgttcggt ctccgctttt tcaacggcgg
     1801 tatatgctgc acaattgatg attacatcaa cgtcgttatc cgataaaaaa tttttaacgg
     1861 catctgcgtc tgtgatatcg agttcggcga catcggtaaa gatgaaatca tggtcgggat
     1921 actgcggcgc aaggagcctg aattcgctcc ccagttgtcc gttgctgcct gttattagga
     1981 tatgcatggc gtttcgtgtt agagttttat gattttgtgc gctgaaatat gatctgaagt
     2041 gtgggcttcc aggtaatata ttccttttgg gagagaagcc ggcagtgaga tgcagaagga
     2101 tgaagatcct tttgcaatac tgtatccgga taacacttgc cctgcaacgt cgataagatg
     2161 gacttcaagg acgctgtttt tcaggtattc cggaataaat atcgtgattt tatcggtgca
     2221 gggattcggg tagataaaaa tttcagggtt tgccgagttt ttatgatccc atgaataacc
     2281 ctggaattcg taagcgccca tatccacgcg gttttcaact acttttggca tccctgccag
     2341 gtctatgggc ggcagacctg ttgtatcggg atcgcctgtg ttgatgcagg ggctgttgaa
     2401 ggcaagacgc aaatcgtcaa tactgatgaa ggaagggtct tcaccgatca ggttggtggt
     2461 atccgtgatt tcaaaaccaa caggctttcc caccagggag tggctaaaaa tatatgatcc
     2521 gtcataaaca cttacatcgg acaggtttgt gctgacttgc aaatagttgc caaaaataat
     2581 gctgttccgg acggaggtaa cggcgttgtt cagtttcata ccgcctcccg tttcattcgg
     2641 gctcattgaa taattacggg tgatagttga gctgagtata tccaggttgc ctgcactcag
     2701 tattccgcca ccggaaccgg caggcgtacc gtatctgacc ttattgccgg aaattatgct
     2761 gttgatgatg gatgtggaac taccggatat aatgcagagg gctccgccac ttccgccggc
     2821 agcccaccag ccggatcccg gattcaacgg atcgggacaa aatccgccat atccggatga
     2881 atttcccgtc agtttgcaat tttctatttt cagataatgc ccgctccaga ttccgcctcc
     2941 gcttcctccg ttggatggga agcaatcata catggaatta tccgatttta cgatggtttt
     3001 tcttccccct gttccggctg aattgttata aatgtccgaa tttttgattt ccagatggcc
     3061 ataattagcg atagcccctc cactggcgcc gggtggggca gggtaggagg cggaatagcc
     3121 caggtcagga ccggaggcgg caagattgga atgaagtatg cagtgatcga tatacaaata
     3181 actgagggta tcgttaatct ggattccccc gccgggttgc ggatataccc ctccgataca
     3241 tattgacttc ccgttgcaga tcaccaggtt tttaaaataa acaaccccgc tgttgaccag
     3301 cctgaaaatc ctgaaatcag gggttccggc tgccggatcg cgacgaatca cggttttgtt
     3361 ttcgttgccc aagatgcaga gcggttgttc aattttcaat tcatcggatg tcagcgtaac
     3421 ggtgtcaaca tcagccgaaa agaggattgt atcgtacggg ccggatattt cgatgaaatg
     3481 gcgcaatgac agatccccgc tgtcgctgcc gttcataaca atatatgttt ccccctttgc
     3541 accggccatg catgagaata atacaagaac aaaaaacaaa taattcattt tcataatgat
     3601 acggttcgga ttattggtta taagttgtaa aaggcacaga cagcttgttt tatcttttac
     3661 tgtccgtaac cgaatgatat ttatttaata agtaacacgg aatgaaagat atatccgttt
     3721 tttagctgta gctgataaca aagataagaa atctggtttc tgatcctaac tatgaaggat
     3781 aagaattccg ggaaatcagg aatgataggg taaggagccg ccgatcacgg aatcagaact
     3841 ttcagaaaga ggcttctctt cccatgccag cggcgcgacg gaacgcggaa ggctttctaa
     3901 accgaaatgt gtaaataata tctttaagct gtttgtcctg gtttattcag ggcctcagaa
     3961 tgccagcagg gcggcgcccc cgataaggag caacgcgaac agatatatga tgagcattag
     4021 tatggtaata agtccgatga cggcaaacag gcttttcagg ttcttgcatg cctttgtcag
     4081 gaactggctg tcgtttttcc ggatggcttt ggggacattg gtggcaaaac tgttgagaaa
     4141 taataccggc aacaggaaca cggctgcaaa aattatatac acaaccgaaa ccaggatggc
     4201 cgggatctgg gcaaaaaccg gtatttgcgt ttcaagcggc ttcatgatga aggcgaagga
     4261 taagccggtc aatatcatga atccggtaag cacaaaacct acgatagaga ggaattttgc
     4321 ccatttggca gcgatttgca aatgaattaa ggcttcgggc gtcatcggcg gagttgccgg
     4381 cgatgtaact tcaggggctg tggtttcttc cataatctgt ttctgttttg agtgattatt
     4441 tcggttggtt catccggtca agatcaatca ttagttttgc catgattgaa tcccagatgt
     4501 agtttgatgg ggaatcgagc acatcattgt cggggctgta cgattcccag taattatggt
     4561 tcgcggccag ttgagtcttg acgcagagca gcattttacc ggccagctca cgggctgcgt
     4621 catcgtagcc gtaatttctg agcccgtcga aaaccatgta attccaaagc aaccataccg
     4681 gtccgttcca tttacagcag tagtcgacgt aaggggtata ccagggatcg tcggctgcaa
     4741 gtgtcggtac gccgtatttc ctccagaatt tgccgggatc ggtaagcttt tttaaaagga
     4801 tttccgccct gtcgccgggc gcagcttcgg cccacagcgc caggaatgct ataatttcct
     4861 gccttcgcaa atcacgcgtc ataaaccgga aggagtgatc atttttattt acggagtaat
     4921 aaaagccggt ttccgagtcc cacatgcgtt cattaacaag cctggaaacc gtatccgctt
     4981 ttgccgacca ttcccgggag tctttttccc ggcccagttc tcctgccatg accgaaaggc
     5041 tgcgcatttc cttgacgacc atgagcgtca ggtcaaggca ttcaagttcg tcgctgatat
     5101 cttccttgtc aacatcaagg tatctttcgg ccgaaacctg aaaaacggcg ttgtaccagt
     5161 cgcggacgtt ttcaatgatg ccgtacggcc cccattcgaa ggtgccgtcg tggtcggtat
     5221 cacggttccg gatgagccag ttggtgtatt ttacccccga ttcataggcg tcgcgaagga
     5281 aggatttgtc tttgctgacg gtatagattt cccagttaat ccagttataa aaaggcgccg
     5341 aagtggtcgg catcccttta tgcggataat cctgcatacc gcgcgggccg tggcggtatg
     5401 cgatcaggcc gtcggcgccc tgctgttcca tgtatacgcg ctgtgattcc tccgccgaag
     5461 cggcgtcgag acaggcatac gataacatgc tcagtgattc gtgcagcacc tgatggccat
     5521 ggccccatcc ccataaaggg ttgcgggaaa aaacataaaa gttatgcgac gttttcccgg
     5581 ccggcggcaa catacatccc ctaaccaggt taaatgcgcc caggtatacc agtttatcgg
     5641 atgcgttgtt gaatttcaat cggggaacag cagcaaataa cctttcgttt tcgtcgatga
     5701 acggttgaag cggaatgttt ttaagagcct gaatttctgc catcagcgtg gtttcgcttt
     5761 ccgaaatgtc acagcacccc ctgaaataac ggatatgttt ctgttgcccc ggatccaggc
     5821 taaaccggtt atggagcatt acaaaatcgg tatatccacc ctggatgcaa ttaagcgaat
     5881 catgtgtgtt tttctcagca tagaaatcgc gtttaacgaa attgtacata tcatccaggt
     5941 tcccgaagta gccacctgtg gaatagggtt caaaaccggc agtaaaaata tcccggcatg
     6001 aagtcgggta agggaaaccg ggattcaggc tgctgatgag ccgcgtcagc gattcacggt
     6061 gcgtaaggat ataggcgctg ttttcggcat cccaccggtc gatgaataag gaatcattgc
     6121 caagttccag tgtcgggtac aagtcgaccg tcagcggttt gtccgaattg ttggtgatgc
     6181 tcatatcgac gatggcgatc gaagaactgt acacgaagaa aagttgctgc acttccaatc
     6241 cttcccaggg ctcatattcc atcagcgcca tatcgggaaa tgaggcggtg acgaccggca
     6301 ggcgatgata tttattcatc cggtcgatta cgactgaatt tatccgccag gtactgtaaa
     6361 ttcgtccggc ctggtcgctg ctgaaattga gcggttcgca cggtgaataa taatccattt
     6421 tataggcttt gtcgccatac atccgtgaac ggttgatgcc ggcagcataa gtggtgtaaa
     6481 gcggatcgcc ggcttctgca ttgattttca ggaactcgtc tttgagactg tactttgcct
     6541 gttgggaaaa ggcaggtgtg gtgaagctga aagcaatgac gaggaagaaa aataaatttt
     6601 tgtatttcac ggccttatct ctgacagggt tgccaaaaac cctggtgttt cttgttgtta
     6661 acaaagttaa accaattacg gatttttaat actttaaaaa tgaaaatccc cggcagaaag
     6721 gcttctggcc ggggaaatcg gagtgtacga ggtgcttcag aaaatcactt acattgcata
     6781 aagaagtcgg ggggcaaagc ggaacggttg atggtttcgc cggctttgaa ctggtcaggg
     6841 ttgaacaggt ctttctcagg aatagttgtt gaagttgaga taaccttaaa ctctgtgcgc
     6901 cggttagcct ggtgttcggc tgccgaacaa ggcacgccgt cggaacagcg gtttgtaagt
     6961 ttgtactcgc cgtatccttt agcggtgatg cgcgaaggat caatgccctg ggctatgata
     7021 tatctgactg cggattcggc cctgttctgc gacagtttat cgttatacgc gtacgaaccg
     7081 cggcaatccg tatgggatga gagttcaaca ttgatgggat tatccttcat gatgcggacc
     7141 aggttttcaa gaggcggttc ggcatcagga cggatattgt atttgtcgaa gtcataatag
     7201 atgttttcga tcacgaaagt tttgctttca gccagtttgt cgaggagaag gtcgcgggat
     7261 gcgggagtcg tttccccggc ttttatatgt tcttcaggcc atgtcatgca atcggcgatg
     7321 tagccttttg tggtggcttt gacgataagt tgggcaggtg ttttcaactg atgtgagtaa
     7381 gctcctttgt catcggtttt aagtatgacg atctcgtcag tttcttcgtt aagcacaaat
     7441 accgttgcat tgggcatagg cctgagtgtg gttttatctt ttacaaagcc tgttatcatt
     7501 gccggcagcg gttctgtttc tttaatggga gcgggaggtt cgggcagttt tctgaaggca
     7561 tagatatcgt cccagccttc accgccgggc ctgtttgagg agaacatacc gtaggaagtt
     7621 ccgggcatcc aggccagggc gaaatcgtcg taggatgaat taaggggagc gtagaggttg
     7681 cgcggtacac tccaggaacc atcagagaat tttgagctga agatatcaag gcctccatat
     7741 cccggatggc catcgctggc aaaatagagc gtgccgtcct gctttaccga agggaacatc
     7801 tcgtttcccg gggtgttgat gacggggccg aggttaacgg cattacccca tccttcaccc
     7861 tggcgtttgc acatccagat gtcggttccg ccttgtccgc cgggcatgtc ggaaacaaaa
     7921 tacataacag aagcatcagc agtaagcgct ggatgaccta cattataatc agggctgttg
     7981 aggtaaaact tattcagttt gccccatttt ccattgacgc gggaggcata aaatatcttc
     8041 agcatgtcgg tcctcacgtt gttaattttc ttggcatcgg catcgtggta ggatcgtgtg
     8101 aatgctacaa ccgaatcgcc ggcgaaatag gcagggccgt catggtacgt ttcgttgaaa
     8161 aagcctttaa gggatctcgg gtaacggata ttaccgaaga catcgcctgt tttttcgggc
     8221 ttggtgaaca gtaattttac atatccgcgg ccagtccagc cataggggcg ttcgtcaagg
     8281 acgttccttt cacggtccga agcataggta aatccattgg cataaaacgc ggggccgaaa
     8341 tcgctggctt ccgagttaac atttgagagc gtcttgatct cgaaagccgg cgccatatct
     8401 ttccatgtca cctgcacact atcgcaatat gcggtatagc gtttaatgcg cggatcctga
     8461 ggggcgaggg cagccgcttt atccagggcc tggtttgcct gcgtgtattc acctgcctgc
     8521 ataagcgacc tggcatactc gaaccaggta tcggcgccgg cgttggggat ctggacagcc
     8581 tgactgtacc agcctcttgc atttgcagga tcattctgca ttttgtaaca ctccgccagc
     8641 aaaggaattg ccgtttcccg gtatttttct ttcttgacgg cgtggttgag tattttgatt
     8701 gcttttgaat aattaaagag attcatatta cgcctggccc tttcggtggg ttgggccatg
     8761 acactctgag cgatcagcag cgtggtaaag gcaagcagga acaatttctt cattgtgatg
     8821 ataattttta caataggcaa atatgagcga ttcctgtaag tgaaatttgt ggtgattaga
     8881 aataccttgg ggtatgcatc cttgactgga agacgtcgaa gtcgaagccg aggcggattt
     8941 cgtgcgatcc cctgttctgg cttctgagtt cgttgaacca gacgtcatag gaatagccga
     9001 ggcggatatt ttgggagagg ttaatctcgg tcatcaggga gagagctttt tcggtgcggt
     9061 acgaagcgcc gatccagaag gtattagcga agaggacgct ggcgttgata tcgagttgcg
     9121 ggggggcatt gggaacgaac ttggcgagaa tggaaggcct gaagacgatg ttatcggcaa
     9181 cggggaaagc agcgccggcc atcccgtaga agtgccggag aagtttggtg aactcggact
     9241 tgccgtcgac aatgacttcg gccatttggt tttgcaggag ttgcttgctg gagaagccga
     9301 cgaaatagcg gttggtataa tagtagatgc cgaagttggc atcgggaacg gcttttttcg
     9361 attcctggcc taggaacatc tggtcttcag tattcaggag atacattttt gaccagtcga
     9421 aatatacatg tttataaccg gcctggattc cgaaagagag cttgccttcg gggaagatga
     9481 tcctgtaggc gaaggtagcc agtgcacctg aatagacgga gttcccgatg gcatcgcagt
     9541 atacattggc tcccagtccg atgtgaggat ttctgagagg ggagtggatg gaagcggaga
     9601 gcgtcttggg agccccggca atgccttccc actggtcgcg tccgaggaca tcgactgaca
     9661 gtgcttcgcg gctgcctgcg taggccgggt tcagggccag tttattaaac atatattggc
     9721 tgaacaatgg gtcctgctga gcgaaagcgg aggacgccag gagaaggagc aggatgggga
     9781 ttattttttt catttttcga ggtattatca gtaacagggt gatggatatt gccagatgga
     9841 caggatgccc cggttgcggg ccccggaaga tattctccgg ggcctgaaag ggctattcag
     9901 attctttact gccgcggaca aggatccatc cgtcgtattt gcctaagccg ggaacggtaa
     9961 caatgtagaa gtatgtgccg tcgggaacat ggtttcctga tttctcatag gtgccgtccc
    10021 aaaccacgtt cttgttgtcg taattggcca cggagtagac aatatcgccc cagcggttga
    10081 agatgatgac tttgttttcg gggaagaact caatgttgcc aatgatccag acgtcgttga
    10141 gtccgtcgcg gttgggggat atcccgttgt agaagatgag gttttcacgg gttccgagga
    10201 cggtgatgaa gattgtggcg gtatcgcaaa gcgaaggctg cccgttatcg cagatcacat
    10261 aggtacaggt atcaattccg aagaaattgg cggcgggggt ataggtcacc gtctggttag
    10321 cgttcaggat gatggtcccg ttatgggggt atgtcaggag agaaacggtg atcgggtcgc
    10381 catcgggatc gtagtcgttg tccaggatgc tgatggtaac cggctgttcc atggacgtgg
    10441 tgtcataatc atcgatagca actgggggat tgttcgaatt gaggaacacg gagacggtca
    10501 cctcgtcgga ggaatagcaa ccggaagcca ggtcaaggac cgtaagggtg aaggtcgtcg
    10561 gttgagcgag cggcaatgtc gaggggttag ctaccgaagc atccgagagg aggctttcgg
    10621 gctgccaact ccaggtatac attccactgc caccggatgc gctgccgtcg agggctgtgg
    10681 ccgtgttggc ggggatagac tggtcgagac cggcatttgc gatcacggat tcatagacta
    10741 taagcgaaag ctggtcggta gcgacgacgt tgccgcaggc agcgggaccg ttgacggtga
    10801 gggtgaggat gatggtcccg gattcaccgg cagccggagt ataggtcggc gtaagggttg
    10861 tttcaccggt aagggtgcca gtaccgttcg aagaccagag gattgagctg tagttggagg
    10921 cagaagcgcc gctaacggta aagggtatac tctggcaggt ggaggcgtcg ggtccggcac
    10981 cggcaacagg cgccaggttg atggtgagcg tcaggttatc ctgtatatct ccgcagggag
    11041 ccagtccggt cacattcatg gtaaggatta cggttccggc ctgcatatcg gcggcagatg
    11101 gggtgtagac ggggttgaga agggcagcat cgtcaaacgt tccggttccg gaggtggtcc
    11161 atgcgatggc gctgaagttt tgggctgaag cctggctgat ggcgatgggg gctgtttcac
    11221 aggtgtacag atcaggtccg gcggtagctg ccacgattgg ctgtatggtg agaacagcct
    11281 cgtcggtagc atcaccgcag ggagcggttc cggtgactgt aagagtaagg gtcacgttgc
    11341 cggtttcatc cacagccggg gtataggttg gggtgagggt tgtttcaccg gtaagggtgc
    11401 cctgtccgtt gtgggtccag aggatggatg tatatccgga ggcggtggca tcaccgatgg
    11461 tgaaaggtgt gctgttgcaa atcgtggctc cttcaccggc aaaagccgaa gtagcaggca
    11521 ggatggtaag tgtcatgtcg tcggcagcgg actcacaaag agctatacca taggcatgga
    11581 gggtgaggac gacgcttcct gcggcgatgt cggctgtact gggggtatag gtggcgtgca
    11641 ggatggtagc attgtcaaag gtgcctgtac cggcggaggt ccaaaggaga ctttcacctg
    11701 cagcagcaga tcctgacagg gtgtaaggag ctgcggcaca ggagatgtca tcggggccgg
    11761 cattggcagc aggagccgta gcgaaggtaa gggtcatggg gtcgctgatg tcgccgcagg
    11821 gggtactacc gaaagctgta agggtaagga tcacactgcc ggcggcgaga tcggcctggc
    11881 tgggggtgta aacaggatgg atgacgccgg gattgtcaaa agtaccggta ccggatgttg
    11941 tccagagaat gctgttgtag ttggaagcat aagcggtaac ggggacaaac gtctggtcgc
    12001 tgcagatggt gctgttttca ccagcggtag ccacaggctg gggcaggatg gagagcagca
    12061 tctgatccga agagtcggcg cagggtgtgt tggcgtaagc ggtaagggta aggtatatat
    12121 ttccggtttc gccgacctga ggaacataag taggggtaag cgtgttggca ttcaggagag
    12181 atccctgtcc gctatgggtc cagaggacgg aagcgtagtt ggtggccgaa gcaccggtaa
    12241 cggtgaagtt gtcggcctgg cagatggtct gatcgggacc tgcgttggca gtgggatgag
    12301 cgatgatggt gaccgactgg atgcaggtgt cggcgttgtt atggatatcg atggcgatcc
    12361 aggtaacata ggttacgccg acggggaaaa cagcgggggc attattggtg ataaccggat
    12421 tgggatcgca gttgtcggta gcggtagcct gtccgaggac aacaggttcg gtgtcgcaaa
    12481 gtacaaggtc ggcgggacag gttatgacag gctgctcgtt atcgacgacg gtgacggtca
    12541 tggagcatga atcggcattg ccgcactcat cggtagcaat ccaaaggatg gtggtagttc
    12601 cgacagggta cacgcccgaa gcattggaag actggttgaa gctgttcaca agggtcacag
    12661 aactgcaagc ttcaacaaca gcaggaacag gaacggtaac agtggcgctg cacagtcccg
    12721 ggtcgttgtt gacgatgatg ttttccgggc aggtgatagc cggtttggtg gtatcctgga
    12781 tggtgaaggt agccgttgtg gtggcgaagt tgcc
//
LOCUS       DAOPYV010000075        12752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_5456_length_12752_cov_11.020005, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000075 DAOPYV010000000
VERSION     DAOPYV010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12752
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5456_length_12752_cov_11.020005"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..382)
                     /locus_tag="PKI35_09590"
     CDS             complement(23..382)
                     /locus_tag="PKI35_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44676.1"
                     /translation="MKKATLYNLVKFSALAIGLLSSLFFLLFLVGEGVGDFLEGKTGA
                     VPILIMMLFTVLGFAISWFKVRIGAVMMIIGALIMGAYLLAVGESGNLLMALAFSVPF
                     LVPGIMMMCLKRLSGHN"
     gene            complement(384..971)
                     /locus_tag="PKI35_09595"
     CDS             complement(384..971)
                     /locus_tag="PKI35_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNX44677.1"
                     /translation="MKKYFLVLLPALMMLSSCSVLQEAGKFANIVNCRFRLESVKQLT
                     LAGVNVQNVKKLSDLTLVDGAKLATAVASGQFPLTFTLNTEVSNPNASSAKMTQFDWI
                     LLIDDIEMTAGTVNNSYTIPAKGASVIPMQMNIDLKKALSGKSADAIINFGLNLAGTG
                     NEPTRFTLKVRPTISVGAYSITYPNYLTVTTQYGS"
     gene            1140..1538
                     /locus_tag="PKI35_09600"
     CDS             1140..1538
                     /locus_tag="PKI35_09600"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNX44678.1"
                     /translation="MAKIVTYSEAASIGMHGMVLIARAGEIMNIQKITEITGSSKHHV
                     AKVMQRLVKDGLLISNRGPQGGFMLNEEPKNITLLRIYESIEGEVKILECPHEKPVCP
                     FGKCILGNVVPQMTTLFRDQLASTTLFDLL"
     gene            1720..2637
                     /locus_tag="PKI35_09605"
     CDS             1720..2637
                     /locus_tag="PKI35_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX44679.1"
                     /translation="MLRKIIHISEEKCNGCGLCIPNCHEGALQVIDGKARLVSDLFCD
                     GLGACIGHCPEGAIEITEREAEPYNEILVMEQMVKMGRNTILAHLEHLRDHNEEGFLK
                     EAIAYIRDNKIDLSPEITGQRPVNTAAAFTTLKEAKEQLGESHSAACGCPGSAARDFR
                     NDRDLMKPASENTGCSPAPSELRHWPVQFHLINPNASYFQGADVILAADCVAFTMGDF
                     HNRLLKGKSLVIGCPKLDSNRESYIEKLTAMIAIARINTLTVAIMEVPCCGGLMQIAE
                     SAVQKAGRKIPVKKIVVGLKGDILSEEWV"
     gene            2804..3130
                     /locus_tag="PKI35_09610"
     CDS             2804..3130
                     /locus_tag="PKI35_09610"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX44680.1"
                     /translation="MKINAYHSVPAMSSGHDARVMHSSPGLEVVHLHLLPGEAVAQHV
                     NNIDIVMCLLQGNTTIVSGDHTYNLNPFDVIEIPAGVERGVVNSDDGDARILVLKKIQ
                     QHLPAR"
     gene            complement(3166..3486)
                     /locus_tag="PKI35_09615"
     CDS             complement(3166..3486)
                     /locus_tag="PKI35_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNX44681.1"
                     /translation="MRYITPAELKQKLDRNEKVQIIDTRSPGEFRKCHIGGAINIPQI
                     DMPESLGLINTEGLVVVYCLYGLKSEAPYLYLREKIKRNNVVILEGGLCRWAAEIDQT
                     LSIS"
     gene            complement(3600..3836)
                     /locus_tag="PKI35_09620"
     CDS             complement(3600..3836)
                     /locus_tag="PKI35_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44682.1"
                     /translation="MKKFFAFLAIAGMVTFVACGPSAEEKAEKARQDSIRMADSIAQV
                     EASAQAQVDSLAKVAADSAAAAAHADSVAKKLIK"
     gene            4241..5518
                     /locus_tag="PKI35_09625"
     CDS             4241..5518
                     /locus_tag="PKI35_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNX44683.1"
                     /translation="MTVVAPPLAEVLRPKTLDEYIGQQHLVGKDSILRRMLEAGSMHS
                     MIFWGPPGVGKTTLANIIALSTIRPFYTLSAINSGVREVREVIGNARNDGTQPILFID
                     EIHRFSKSQQDSLLGAVEKGIVILIGATTENPSFEVISPLLSRCQVYILKPLEEADLM
                     IIAERARILLEKQFETTITISENEALLRLSGGDARKLLNAMELVVKSQHAPGVSVTID
                     NKTTLEVIQQNLALYDKAGEMHYDVISAFIKSLRGSDPNAAVYWLARMITAGEDLLFI
                     ARRMVILASEDIGNANPNALLLANACFDAVNKIGYPESRIILAQTAVYLATSPKSNAS
                     YMAIEEALAAVQKSGDLPVPLHLRNAPTGLMKDLGYSKNYQYAHDFEGNFVEMEFMPA
                     GLSGKKFYDPGKNARENDTRERLKRLWKDRYGY"
     gene            5558..6382
                     /gene="kdsA"
                     /locus_tag="PKI35_09630"
     CDS             5558..6382
                     /gene="kdsA"
                     /locus_tag="PKI35_09630"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNX44684.1"
                     /translation="MSDQVLKQIPLLHNTESGNFFLLAGPCVIEDEISPFGIAENIKR
                     ITEKLAIPFIFKASYRKANRSSSHSFQGIGDLNALKVLAAIRKEYGVPVVTDIHSVPE
                     AGLAAEYVDMLQIPAFLCRQTDLLQAAGRTGKIVNIKKGQFISGESMRFAVEKVCETG
                     NRNIILTERGNTFGYNDLVVDYRNIPAMQKTGFPVVLDITHSLQQPNQSSGVTGGRPE
                     MIETIAKAGIAAGADGIFIETHPSPSQAKSDGANMLQLDKLEELLEKLIRIRKAIQ"
     assembly_gap    6607..6706
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6749..7369)
                     /locus_tag="PKI35_09635"
     CDS             complement(6749..7369)
                     /locus_tag="PKI35_09635"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNX44685.1"
                     /translation="MKLKEVINKAVRITIIPSHQWKIVRDEACTPKSLLWEYGFPIIL
                     FSSIGRTIGVLFSTLPVLGFSLKLISVLLFNLVSWVAIPYILILSAAYVLKWGLPEMG
                     IENDLIKSLKLVLYTFTPVFIFTFFVYLHPLLRILIPMGIYVFIAYTLYVFWYGVREL
                     FEVPLEKKIGLIVVSIVLAFGAMFLAQHLYGLLIDLIIPGMETYVK"
     gene            complement(7406..8587)
                     /locus_tag="PKI35_09640"
     CDS             complement(7406..8587)
                     /locus_tag="PKI35_09640"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HNX44686.1"
                     /translation="MKFLIMAKSRIPLQVIAVAALLGFFSLQAAAQVSVVKVGSEIID
                     PMSDGLFYSLPRTVIKVDLVVSKITNVRGPYAEFADKYLGLSQVVMTNSTEYSLSEIR
                     LSSYEESDPSEYYFVKVPEKSKSRQAVELSLSQLGTLVGAKGSSAGEDRGKSVKIEPS
                     PDVMNEMPNPNTVERIDTLIRRISVDTTMIEQRVFKKVTAAKTTEQKAKEAADFIIKL
                     DESKFNLINGYQEVSYEKGTMEYMYNKMEQLMSDYLQMFKGIRLVETETYSFTVVPAT
                     ESLDKPVPVCRFSPGKGILDKGAAGGDLVQVRVEASNSLSQVRTPVQDRNARVKSEKG
                     LYYRIPEKAAVSVLIGGQPRVDGKFTVSQLGVVTFLPAGSTGNIELYPGTGSVKHITL
                     Q"
     gene            complement(8806..10995)
                     /locus_tag="PKI35_09645"
     CDS             complement(8806..10995)
                     /locus_tag="PKI35_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNX44687.1"
                     /translation="MSDVRFKALELAVSRPKRAIELPSNKVSDYFGSLTFNRAVMREY
                     LSKEAYDKLISVIEKGEKIDRRVADQVATALKSWAIAKGATHYTHWFHPLTGATAEKH
                     DTFMVPTPSGKAIEEFNSNQLIQQEPDASSFPSGGIRNTFEARGYTAWDPSSTAFIID
                     DTLCIPTIFISYTGEALDYKTPMLKALHALDKEAVAVCKYFDRNVNKVTATLGLEQEY
                     FLVDTALYQARPDMALTGRSLFGHASAKDQQLSDHYFGTIPERVADFMTEFEYEAHRL
                     GIPLKTRHNEVAPSQFECAPVFEEVNVAVDHNQLLMHTMEKVARRHNLTVLFHEKPYH
                     GINGSGKHNNWSLMTNAGDNLLSPGKTPKSNLRFLTFFVSIIKAVDTYGDLLRMSIAS
                     VGNDLRLGAHEAPPAIMSVFIGSQLTETLDAIEKGSEREAAVNGKKEDTAINIPKIPE
                     ILPDNTDRNRTSPFAFTGNKFEFRAVGSSMNCARPMIILNLIVAEQLRLFKEETDALI
                     AKGLVAEDAVYKVIRKYIRESKRIRFEGNSYSQEWIDEAKKRGIQNIPSTPDALKVMI
                     EPGIISLFERHHVLTKREMEARYDINLENYIKKIQIESRVIGDLAINHIIPTAIRYQN
                     MLIENVRGLKEVLDNKTFVRLSNNQMMNIKEISEHISIIRTLVNEMVETRKVANKFEN
                     PVEKADSYKDSVLPYFDKVRYHVDKLEFLVDDELWPLPKYRELLFIK"
     gene            complement(11089..12537)
                     /locus_tag="PKI35_09650"
     CDS             complement(11089..12537)
                     /locus_tag="PKI35_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNX44688.1"
                     /translation="MTPSLIFTVFIAYTALLFLVTWITARRATNFSFYLGNKASPWYV
                     IAYGMIGASLSGVTFMSVPGWVGTTQFSYLAVILGYVVGYAVIATVLMPLYYKLNLTT
                     IYTYLEQRFGFWSYKTGAFFFLLSRVIGASFRMFLVVNVLQIFVFDAWHIPFAATVAI
                     FITLIILYTFKGGIKTIIWTDTLQTTFMLAAVGISIYLISKDFQWGVRDLFSNVLSSD
                     YSQVLVTDWHDKRHFLKQFLSGAFITIAMTGLDQEMMQKNLSCKNIHDAQKNMFTFSI
                     VLVFVNLMFLFLGATLFLFSAKHGIELPMRSDDLFPVIAMKYLGPFAGLTFIIGLISA
                     AYPSADGALTSLTTSFSIDFLGLNKRDDLTEKKKQKIRYSVHFSFALLLLLVIIIFRA
                     INDQAVIDKLFTVAGYTYGPLLGLFSFGLFSKRNVKDRFVPYIAALSPVICYFLSEYS
                     MVLFNGYKFGFELLIVNGLLTFIGLFLVSKRD"
BASE COUNT         3524 a         3021 c         2861 g         3246 t
ORIGIN      
        1 ttttttattg cgatagcctg tgctaattat ggccggaaag ccttttgagg cacatcatca
       61 tgatgccggg caccagaaaa ggaaccgaaa aggccagggc catcagcaga ttgccgcttt
      121 ctcctacggc cagcagatac gctcccatta tcaaggctcc aattatcatc atcaccgctc
      181 caatccttac tttaaaccat gagatggcaa atcccaggac agtgaacagc atcataatta
      241 aaatcggaac agcccctgtt ttcccttcca gaaaatctcc gactccttca cctaccagga
      301 aaagcagaaa aaaaagcgat gagagcaaac cgatagccag ggctgaaaat ttaaccaaat
      361 tgtataaggt tgcctttttc atcttacgat ccgtattggg tggtgacagt caggtaattc
      421 ggataagtta ttgaataagc gccgacagaa atcgtcggcc tgaccttaag ggtaaaacgg
      481 gtaggctcgt tgcctgtccc ggcaaggttt aatccgaaat taataatggc atcggcactt
      541 tttcccgaca atgctttttt caggtcaatg ttcatctgca tcgggataac agaggcaccc
      601 tttgcgggaa tggtgtatga attattaacg gtgccggcag tcatctcgat atcatcgatc
      661 agcaaaatcc agtcaaactg cgtcattttg gctgaagaag cattgggatt gcttacttcg
      721 gtattcagtg taaaagtgag tgggaactgg cccgaagcaa cagccgttgc cagtttggct
      781 ccatcaacga gggtgagatc cgaaagtttt ttgacattct gaacatttac tcccgcgagg
      841 gttaattgct taaccgattc gagccggaat ctgcaattga caatattggc gaactttccg
      901 gcttcctgaa gtacgctgca cgaactcaac atcatgagtg cgggaagtaa cacaagaaaa
      961 tattttttca tgtttcagtt aaattttcga cgtcacaaaa gaacaaaaac tataccctgc
     1021 tttggactgt atccttaatt tagaatcttt tgaaattgca ggattcttaa aacatgttta
     1081 attaattacg acatttgagc aattaattac ctgattgatc taaattacca ctttttgaga
     1141 tggcaaaaat tgtcacatac tccgaagcag catccattgg catgcacggc atggtactca
     1201 tcgccagggc gggtgagatc atgaacattc agaaaataac cgaaatcacc ggatcatcga
     1261 aacatcacgt ggcgaaagtc atgcagcgcc ttgtgaaaga cgggctgctg atttcaaacc
     1321 gcggcccgca gggaggcttt atgctgaatg aagagccgaa gaacatcaca ctcctccgga
     1381 tttatgaatc tattgaaggc gaagtgaaaa ttctggaatg cccccatgaa aagccggtat
     1441 gccctttcgg caaatgtatc ctgggaaatg tcgtacccca aatgaccact ttgttccgcg
     1501 accaactcgc atccactact ctttttgacc tgctgtaggc cagcttgtgc cgatgttgta
     1561 ataccatgat ttttttacgt atacgaaaag ccttttcaga aaaaacacct acatttgcgc
     1621 attctattac ttaatttcct aattcattcc ggtgaattgc taacaaactg attttctttt
     1681 aatttcaata gcagaaacaa aaaaattaac attaaaacca tgctcaggaa gatcatccac
     1741 ataagcgaag agaaatgcaa cggatgcgga ttatgcattc ccaactgcca tgaaggcgca
     1801 ttgcaggtca tcgacggtaa agcgcgcctg gtaagcgacc tgttctgtga cggactgggt
     1861 gcctgcatcg gccattgccc cgaaggtgcc atcgaaatca cggaacgcga agccgagcct
     1921 tacaacgaaa tactagttat ggagcagatg gtaaaaatgg gccggaatac aatcctggcc
     1981 catctggaac acctccgcga ccataatgaa gaaggtttcc tgaaagaagc catcgcttac
     2041 atcagggaca acaaaatcga cctgagcccc gaaataaccg gacaaagacc ggtaaatact
     2101 gccgctgctt tcaccaccct gaaagaagca aaagaacaac tcggggaaag ccatagcgcc
     2161 gcctgtggtt gcccgggatc agcagcaagg gatttccgga atgacaggga ccttatgaag
     2221 cctgcttcag aaaataccgg ttgttcgcct gccccgtctg aactcaggca ttggcctgta
     2281 cagtttcacc ttatcaatcc caacgcttca tactttcagg gagcagacgt gattctggca
     2341 gccgattgcg tcgcatttac catgggggac tttcacaaca ggttgctgaa aggaaaatcg
     2401 ctggtcattg gctgtccgaa gctggactct aacagagaat cctatattga aaaactcaca
     2461 gccatgatcg ccatcgccag gatcaacacc ctcacggtcg ccatcatgga agtgccatgc
     2521 tgcggaggat tgatgcaaat tgcggaatca gccgtacaaa aggccggacg caaaataccc
     2581 gtaaaaaaga tcgtcgttgg cctcaaaggt gatatccttt cggaagaatg ggtttaatca
     2641 attgaatagc tgtttattat caaaataata cagccaacat aaagcggatg tcattcccgc
     2701 gtttccctga tgacggacac aattccggat accggggagg gcaaattttc agccgggaaa
     2761 ggcagaaatc aataactgtc agcatcaata tctttaaagc atcatgaaaa tcaatgccta
     2821 tcattccgtt cccgcgatgt cttccggaca tgacgcccgt gtcatgcatt cgtcgcctgg
     2881 gcttgaagtc gttcatcttc atctgctccc cggagaagcg gtagcgcaac atgtaaacaa
     2941 cattgatatc gtaatgtgcc ttttacaggg caacacaacc attgtgtcgg gagaccatac
     3001 atacaatctg aaccccttcg atgtgataga aataccggca ggcgttgaac ggggtgttgt
     3061 aaattccgat gacggtgacg caagaatttt agtcctgaaa aagatacagc agcatttacc
     3121 ggccagatag ttaccggcag ctttgggatt ccgggcgcct ggatatcagg aaatggaaag
     3181 cgtctggtcg atttcggcag cccaccggca caaaccgcct tcaaggatta ccacattgtt
     3241 gcgttttatt ttttctcgaa ggtaaagata cggggcttcg cttttcaagc cgtaaaggca
     3301 ataaaccaca accagacctt cggtgttgat caaaccgaga gattctggca tatcaatctg
     3361 aggaatattt atcgcccctc cgatgtggca tttcctgaat tccccggggc tccgggtatc
     3421 aatgatctgc accttttcat ttctgtccag tttctgtttc agttctgcag gagtgatata
     3481 ccgcatagat gtacaataat cgcggtaaaa atatgaaaat cggaagtttc tgatcggata
     3541 aaaataaaaa aaggagccaa atggttcctt ttttaaacga ttatcaaggc tggtatttac
     3601 tacttgataa gttttttggc aactgagtca gcgtgagcag cagcagcggc gctatcggca
     3661 gctacttttg caagtgaatc aacctgagcc tgggcagaag cttcaacctg agcgattgaa
     3721 tcagccatgc ggattgaatc ctgacgagct ttttcagctt tttcttcggc gctgggaccg
     3781 caagctacga aagtaaccat accagcaatt gctaagaatg caaagaattt tttcatgtgg
     3841 tttattaatt aaaaaacgtg gcaaaagtac agcttttcaa tataccaaaa tcatttttct
     3901 taaaaatatt taaatatttt taaattaatt ttatttcgct gtctttctgc tacttataat
     3961 cggccttatt atgaaattta aaccattttt ttcacttatt ttcaaaatcc ggcatttccg
     4021 gccttcaaat catacctgat tgacccgctg actgatcaat tttttaagaa ttctgaagtg
     4081 accttttcgt tgacagaata ctgccttatc aacaatcgct taccgggaat aaatagtacc
     4141 tttgattcgt ttcacggttc cccccgttgt gcattaaatc cggcagcgat gcgatcctgt
     4201 cagctttata tgaccaacca gaataaatga tccttagctc atgactgttg ttgcgccgcc
     4261 tttagccgaa gtattgcgtc cgaaaacgct cgatgaatat atcggccagc agcatcttgt
     4321 cgggaaagac tccattttaa gaaggatgct cgaggccgga tccatgcatt ccatgatttt
     4381 ctgggggccg ccgggagtag gcaaaacaac gctggccaac attatcgccc tgagcaccat
     4441 acgtccgttt tatacgctca gcgccatcaa ttccggggta cgcgaagttc gggaagtaat
     4501 cggcaacgcc cgcaacgacg gcacacaacc tatcctgttt attgacgaaa ttcaccgttt
     4561 cagcaagtcg cagcaggatt cattgctggg tgcagtagaa aaaggcattg ttattctcat
     4621 cggtgctact accgaaaacc cttcctttga ggtgatctcc cccctgctgt cgcgttgcca
     4681 ggtatatatc ctgaaaccgc tcgaagaggc tgatctcatg atcattgccg aaagggccag
     4741 gatccttctt gaaaagcaat tcgaaacaac aatcacaatt tcggagaacg aagcattgct
     4801 caggctctcc ggcggcgacg cccgcaaatt gctgaatgcc atggagctgg tggttaaatc
     4861 gcaacacgcc cccggagttt cagtcaccat cgacaataaa acgacgctgg aggtcatcca
     4921 gcaaaatctg gctttatacg ataaagccgg tgagatgcat tacgatgtga tctctgcttt
     4981 cattaaatcg ctccgcggca gcgaccccaa cgcagcggta tactggctgg cccgcatgat
     5041 caccgccggc gaagaccttc tttttatcgc ccgcaggatg gtaatcctcg cttcggagga
     5101 catcggcaat gccaatccga atgccctgct gcttgccaac gcctgttttg atgccgtaaa
     5161 taagatcgga tatccggaaa gccggatcat tcttgcgcaa acggcggttt atctcgccac
     5221 ctcgcccaaa agcaatgctt cgtatatggc cattgaagaa gctctggccg ccgttcaaaa
     5281 atccggagac cttcccgtac ctttgcacct tcgtaacgcg cctaccggtc tgatgaaaga
     5341 tctgggctac agtaaaaatt accaatacgc ccacgatttt gaaggcaatt tcgtggaaat
     5401 ggaattcatg cctgccggcc tttcgggcaa gaagttctat gatcccggga aaaatgctcg
     5461 tgagaatgat acccgggaac gcctcaagag gctttggaag gatcgttacg gttattaaac
     5521 atgaaataaa tcattgctcc ataacacctt aacaattatg tcggatcagg ttctgaaaca
     5581 aattcctctg ctccataaca ccgaatcagg aaattttttc ctcctggcag gcccctgcgt
     5641 gatcgaagac gaaataagcc cattcggaat cgctgaaaat attaaacgca ttacggaaaa
     5701 acttgccatc ccgttcattt tcaaagcttc gtaccgcaag gcaaaccggt cgagcagcca
     5761 ctcgttccag gggatcggcg atctcaacgc cctgaaagtt cttgctgcaa tccggaagga
     5821 gtacggagtg cctgtggtta ccgatataca ctcggtaccg gaagccggat tggccgccga
     5881 atatgtggat atgttgcaga ttcctgcatt tttatgccgc cagactgatt tgctgcaggc
     5941 tgcagggcgg accggtaaaa ttgtaaatat caaaaaaggt caattcattt ccggagaatc
     6001 catgcgcttc gctgttgaaa aagtatgcga aaccgggaac cggaacatca ttctcaccga
     6061 gcgcggcaat actttcggat ataatgacct cgtggtcgat taccgcaata ttcctgcgat
     6121 gcaaaaaaca ggtttccctg ttgtgctcga tataacccat tcgctgcagc aacccaacca
     6181 gtcctcgggc gtcacgggcg gccggcccga aatgatagaa accattgcaa aagcgggcat
     6241 cgccgcaggc gccgacggga tatttatcga aacgcatccc agcccgtcgc aggccaaatc
     6301 ggacggggca aatatgcttc aactggataa actggaagaa ctgctcgaaa aactaatccg
     6361 gatcagaaag gctattcagt aaccatgatg gaataccgga aagctgaatc cggaaaagga
     6421 gaacggagaa cgagggacac aaaacggaga acgcgaatca cgaacaacga atcacgagcc
     6481 ccgaatttca cgattgctat cacgaatacc acgaattact aattactaat tactaatcac
     6541 gaacaacgaa tcacgatact caggattcaa agcgccaaca gcctgcctgc cctggctggc
     6601 tgtgcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccga caccgtccct
     6721 tatcatccaa taataaacaa gggctgccct atttcacata ggtttccata ccgggaatga
     6781 taaggtcaat cagcaatccg taaaggtgct gagccaggaa catggccccg aaggcaagga
     6841 caatcgatac cacgataagg ccgatcttct tttcaagcgg aacttcgaac aactcccgaa
     6901 cgccgtacca gaaaacataa agggtatagg ctatgaaaac atagattccc attgggatca
     6961 ggatgcgaag caacgggtgc aggtagacga aaaaagtaaa gataaaaacc ggggtaaaag
     7021 tgtagagtac taatttcagc gactttatca ggtcattttc aattcccatt tcaggcagcc
     7081 cccatttaag cacataagcc gccgaaagta tgaggatata aggtattgcc acccaggata
     7141 ccaggttgaa caataatacc gaaatcagtt tcaggctaaa gcccaatacc ggcaaggttg
     7201 aaaaaagtac gccgatcgtt cgtccaattg aactgaaaag tatgatcggg aatccatatt
     7261 cccataacaa actctttggc gtacaggcct catccctcac gattttccat tgatggctgg
     7321 gaataatcgt aatacgaact gccttgttga taacctcctt taatttcatt aagatataga
     7381 ttgagtattc cttgctgtga tgagtttact gcaatgtaat atgttttaca cttccggtac
     7441 ccggatatag ttcaatattg cccgttgaac ctgcagggag gaaagtgacc acccccagtt
     7501 gactgactgt aaatttaccg tcaacgcggg gttgtccgcc tatgagaact gaaacggcag
     7561 ccttttcggg aatcctgtaa taaagcccct tttcggattt cactcttgca ttgcgatcct
     7621 ggacaggagt ccgtacctgg gataatgaat tggaagcttc gacccggacc tggacaagat
     7681 cgccgccggc agcgccttta tccaggatgc ctttcccggg cgaaaacctg caaaccggaa
     7741 caggcttatc caggctttcg gttgccggaa caacggtaaa ggagtaggtt tcagtttcca
     7801 ccagccggat tcctttaaac atctgaagat aatcgctcat cagttgctcc atcttgttgt
     7861 acatatattc catggttccc ttttcatagc ttacctcctg atagccattg atcaggttga
     7921 acttgctctc gtcgagtttt atgatgaaat cagccgcttc ttttgctttt tgttcggtgg
     7981 ttttggctgc cgtcaccttt ttaaagacgc gttgttcaat catggtggta tcaacgctga
     8041 tgcgcctgat cagggtgtcg atcctttcaa ccgtgttagg gttgggcatc tcattcatta
     8101 catcgggcga tggttcgatt ttcacggatt tcccgcgatc ctcccctgca ctgcttcctt
     8161 ttgcgccaac cagcgttccc agttgcgaca gcgacagctc aacggcctgc ctcgattttg
     8221 acttttccgg tacctttaca aaataatatt ccgagggatc agattcttca taggatgaca
     8281 acctgatttc ggacagcgag tattcggtgc tgttggtcat cactacctgg gaaagcccca
     8341 ggtatttatc ggcaaattcg gcataaggcc ccctcacgtt ggtaatttta ctgaccacca
     8401 gatcaacctt gattaccgtg cggggcaggg aataaaagag cccgtcgctc ataggatcaa
     8461 tgatctcgct gcctacttta acgacgctaa cctgcgctgc cgcctgcaac gaaaagaaac
     8521 ccaatagtgc ggcaactgca attacttgaa gaggaattct gcttttagcc ataatcagga
     8581 atttcatttc attttcaaag atagaaaaga atcagtttaa attaataatt gccgggccaa
     8641 atgataaccg gcacaacctg actgttccct gatacctaac ggttttgtga aaataatgtt
     8701 gctttccggc gttgataaac agataaagct cccggttttt aacccggacc atatccttca
     8761 gcacttataa aagccatatc tcgcggtaaa caatgcctgg tataattatt tgataaacag
     8821 cagttccctg tacttcggca acggccataa ttcatcatcg accaaaaatt ccagcttatc
     8881 cacatgatac cttaccttat cgaaatatgg cagtacggaa tctttatagc tatccgcttt
     8941 ttctacgggg ttttcgaatt tgttggcaac cttgcgggtt tcaaccatct cgttcaccag
     9001 cgtgcggata atggagatat gttccgaaat ctccttgata ttcatcatct gattattcga
     9061 aagccttaca aatgtcttgt tatcaagaac ttccttcagc ccgcgcacat tttcgatgag
     9121 catgttctgg taccggatag ccgtcggaat gatatggttt atggccaggt cgccgataac
     9181 ccttgattca atctgaattt tcttgatata attttcaaga ttgatatcgt acctggcttc
     9241 catctccctt tttgtgagca catgatgtct ttcaaagagg ctgataattc cgggctcaat
     9301 cataaccttc agcgcatcag gcgttgaggg aatgttctga atcccccttt tcttggcttc
     9361 atcaatccat tcctgactgt aactgtttcc ctcgaaacgg attcgttttg attccctgat
     9421 atatttacgg atgaccttat agaccgcatc ttcggcaacc aggcctttgg caatcagggc
     9481 atccgtttct tccttaaaca gccttagctg ttcggcaact atcaggttca gtatgatcat
     9541 cggcctggca caattcatgg atgagccaac ggccctgaat tcgaacttat tacctgtaaa
     9601 agcgaaaggt gaagtgcggt tacgatcggt attatcgggg agaatttcag gaattttcgg
     9661 aatattgatg gccgtatctt ccttcttccc attcacagcg gcctcacgtt cagatccctt
     9721 ttcaattgca tcaagggttt cggtaagttg tgaaccgata aacaccgaca taatcgccgg
     9781 cggcgcctca tgagctccaa gtctcaggtc gtttcccact gaggcaatgg acattcgcaa
     9841 caggtcgcca tacgtatcga cagccttgat aatgctcaca aagaatgtaa ggaaacgcag
     9901 gttcgattta ggtgtttttc ccggcgagag aaggttatcg cccgcattgg tcatcaggga
     9961 ccagttgttg tgctttcctg atccgttgat accatggtaa ggcttttcat ggaaaaggac
    10021 agtaaggtta tggcgcctgg ctactttctc catagtatgc attaatagct ggttatggtc
    10081 aacggcaaca ttcacttcct cgaataccgg ggcgcattca aactggctgg gcgccacttc
    10141 gttgtggcgg gttttcaaag gtatgccaag ccggtgggct tcatactcaa actcggtcat
    10201 aaaatcggct acccgttcgg gaatcgtccc gaagtaatga tcggaaagct gctgatcctt
    10261 ggccgaggca tgtccgaaca acgaacgacc ggttaacgcc atatcgggac gggcttgata
    10321 caacgcggta tcaaccagaa aatactcctg ttccaatccc agggttgctg tgactttatt
    10381 cacgttccgg tcgaaatatt tgcatacggc aaccgcttct ttgtcgaggg catgcaaagc
    10441 cttgagcatc ggcgttttat aatcaagggc ttcgccggta taggagataa agatggttgg
    10501 aatacaaagg gtatcgtcga taatgaaggc tgttgacgac gggtcccagg cggtatatcc
    10561 ccgggcttca aaagtattcc ggataccccc gctgggaaag ctcgatgcat ccggttcctg
    10621 ctgtatcaac tgattgctgt tgaactcctc tatggccttc cctgaaggtg tcggaaccat
    10681 aaaagtatca tgtttttcgg ctgtcgcccc tgtcagcgga tgaaaccaat gcgtataatg
    10741 ggtagcgcct tttgcaatgg cccatgattt taaggcggtg gcaacctggt cggcaacacg
    10801 gcggtcaatt ttttcccctt tttcaattac actgatcagc ttatcataag cctcttttga
    10861 taagtactct ctcatcaccg cccggttgaa cgtcaatgac ccgaaataat ctgaaacctt
    10921 gtttgatggg agctctatag ctcttttggg gcgcgaaact gctaattcca gcgccttaaa
    10981 acgcacgtcc gacatatgaa agatttttaa ttttgagtct gcaaaattac cgattagaat
    11041 ctgtaaaatt ttacagtgaa tctcttaatc ttccgataac aacaaaattt aatctctttt
    11101 gctgacgagg aaaagcccga taaatgtcaa caatccgttg actatcaata gttcaaatcc
    11161 aaatttataa ccattgaaca aaaccatgga gtactcgctc aggaagtaac aaatcaccgg
    11221 tgaaagcgcg gcaatatagg gaacaaaacg gtctttcacg tttcgtttgc tgaagagccc
    11281 gaaactgaac aaacccagca acggcccata cgtataaccg gctaccgtga ataatttgtc
    11341 gattacagcc tgatcgttaa tagccctaaa aatgatgatg accagcagca acagcaatgc
    11401 aaacgaaaaa tgaacggaat accttatctt ttgttttttc ttttctgtga ggtcatcccg
    11461 tttgttcagc ccgagaaaat cgatgctgaa cgaggtggtc agggaggtaa gggcgccgtc
    11521 ggcgctggga taagcggcag agatgaggcc gatgataaag gtaagcccgg cgaatggccc
    11581 cagatatttc attgcaatga caggaaacag gtcatcgctt cgcatgggca attcgatgcc
    11641 gtgttttgca gaaaaaagga acaatgttgc acccagaaag agaaacatca ggttaacaaa
    11701 caccagtaca atactgaacg taaacatatt cttctgggcg tcgtgtatat ttttacagct
    11761 caggttcttc tgcatcatct cctggtcgag cccggtcatt gcgatagtga tgaaggcgcc
    11821 gctcaggaat tgtttcagga agtgcctctt gtcgtgccag tcggtaacca aaacctgact
    11881 gtaatcgctg gacaaaacat tagaaaataa gtcgcggacg ccccattgga aatccttgct
    11941 gataaggtag atcgagattc cgacagcagc cagcataaat gttgtttgca gcgtgtcggt
    12001 ccagataatg gtcttgattc cgcctttgaa agtataaagg atgatcaggg tgataaaaat
    12061 agcgaccgta gcggcaaagg gaatgtgcca ggcatcaaaa acaaaaatct gtaaaacatt
    12121 tacgacgagg aacatccgga acgaagcgcc aataaccctt gacaacagaa agaagaatgc
    12181 gcctgtctta tatgaccaga acccgaacct ttgctcaaga taggtgtata tagtggtcag
    12241 gtttagcttg taataaagcg gcataagaac ggtggcgatc acagcatagc cgacgacgta
    12301 acccaggatc acggcaagat aactgaactg cgtggtgccc acccatccgg ggaccgacat
    12361 gaaggtgacg cccgacaacg acgccccgat catcccgtat gcaatcacat accagggtga
    12421 tgctttattt ccaaggtaaa atgaaaagtt tgtcgcacgg cgggccgtaa tccatgtcac
    12481 caggaacagg agggcagtat aagcgataaa aacggtaaag atgagggagg gagtcaaggt
    12541 gaataagttt gagcagttta atattacggg tttacattct cagaagatga atcattatta
    12601 tcagcagtaa ttctgacgaa gtcgttttat attctttcag catcagggtt ttcgctgcag
    12661 gctgtgggct gttaggttaa ggtttcagtg tttcagcgtt tcagtcctgc ctgccttgcg
    12721 caaggctgtg ggagggggca gggtttcaga gt
//
LOCUS       DAOPYV010000076        12670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_5513_length_12670_cov_10.851367, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000076 DAOPYV010000000
VERSION     DAOPYV010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5513_length_12670_cov_10.851367"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1214)
                     /locus_tag="PKI35_09655"
     CDS             complement(222..1214)
                     /locus_tag="PKI35_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNX44689.1"
                     /translation="MMNVEFANPAYFWLLLLVPAAAGWYWYRLMHLHAKFQVSTFTAL
                     SSVKPGWRVRCRHLLFALRMLALIFIIIALARPQRSMSSQNISVEGIDIILANDISGS
                     MLAEDFRPNRLEAAKDVATRFINGRPNDRIGLVVFSGESFTQCPLTTDHSVLINLYGE
                     IKSGMVDDGTALGDGLATAVSRLKESKAVSKVIILLTDGINNMGAIDPVTAAEIASLY
                     GIRIYTIGIGTIGTAPYPFETPFGIQYQNVEVKIDEALLQKISSMTNGKYFRAVNKRK
                     LESIYQEIDKMEKSKIDVTEFRRKKEEFFPFVLIALVMLGVEIVLRHTVFKTIP"
     gene            complement(1214..2230)
                     /locus_tag="PKI35_09660"
     CDS             complement(1214..2230)
                     /locus_tag="PKI35_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44690.1"
                     /translation="MGSGHHPKTVTPVAFLIVLTVMLTACPVVKAQKAAAKLDTAAIR
                     IGEQVRLQLGFTAPKSVKVQWPVIPDTLSPSVEVVKRGPVDTSAVKGRDLLQYSQYIT
                     ITSFDTGFHSIPPLNFIYRKGSDTAGYNSYTDSLMMQVSTVAVDTTLAIRDIKAPMKQ
                     RLTFGELLPYILGALAVLALAAVVIYYLWRRKQNKPFIPKIVKPALPAWQTALNELKE
                     LSEKKHWQEGRIKLFHSELSDILRRYLEYQHLIPAVEMTTDDILELCSNSPAVSQSTE
                     QLARVLRLADLVKFAKEIPLPPENEQSLKDAILFVDRTKPVVQQETKATTPSDTAMTQ
                     QTTE"
     gene            complement(2241..3116)
                     /locus_tag="PKI35_09665"
     CDS             complement(2241..3116)
                     /locus_tag="PKI35_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNX44691.1"
                     /translation="METSDIIRKVRKIEIKTRGLSHQIFSGQYHSAFKGRGMAFSEVR
                     EYQFGDDIRNIDWNVTARFNHPFVKVFDEERELTVMLLIDVSGSNEFGTRKMFKEDQM
                     TEIAAVLAFSAIQNNDKVGVIFFSDVIEKFIPPKKGTSHILRIIRELVDFRPVSQKTD
                     ISEALRFLTNAIKKRCTAFIISDFFDHDFEKALQIASGKHDIVALQVYDESETNLPPV
                     GLMQVYDKESNASTWVDLSDLQVRQAYTHWWKNHENTLNGIFMRSGVDTVRFGTDQDY
                     VKPLIGLFKKREARY"
     gene            complement(3207..4199)
                     /locus_tag="PKI35_09670"
     CDS             complement(3207..4199)
                     /locus_tag="PKI35_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX44692.1"
                     /translation="MVETNIKELNDRIQTESAFVDMLTMEMHRVIVGQKQMIERLLIG
                     LLADGHILLEGVPGLAKTLAIKSLARTIDLNFSRIQFTPDLLPADLIGTLIYSQKNED
                     FMVRKGPVFANFILADEINRSPAKVQSALLEAMQERQVTIGEKTYKLPEPFLVLATQN
                     PIEQEGTYPLPEAQVDRFMLKVVISYPQKQEEKLILRQNISQETLSTNTILKPEDILK
                     AREVVRQVYLDEKIENYITDIVFASRFPADYKLKNFESLIRYGASPRASIYLALAAKA
                     FAFIKRRGYVIPEDIRAVAHDVMRHRIGLTYEAEAENITTEEIISEILNTVEVP"
     gene            complement(4492..5220)
                     /locus_tag="PKI35_09675"
     CDS             complement(4492..5220)
                     /locus_tag="PKI35_09675"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNX44693.1"
                     /translation="MDTFISDLSHQEFPVMERVSAKTIRHPVLHLMQKENPAINHNSF
                     ISIGELNEYQQRYITNYLTREVGKLDEPEKTVLNSMSANATLSDKLDSERQPKLTAGQ
                     KIADKVATFGGSWKFIIAFMVFILVWIAINIYWLANTGFDPYPFILLNLILSCVASIQ
                     APVIMMSQNRQEEKDRERAKNDYMINLKSELEIRSLHEKVDHFIMDQQQELFELQKLQ
                     VSMMNEIVSIVTNNAGSPVKKISG"
     gene            complement(5249..6226)
                     /locus_tag="PKI35_09680"
     CDS             complement(5249..6226)
                     /locus_tag="PKI35_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44694.1"
                     /translation="MKFETLIILSWMMLTCNPGNSKPVTDYQLFTGWGTLRRNSEKVI
                     VIRQFKRDNQKFYLTVSPETLLTRVINADSIKVISGSWQTVSSRFRNTPYIAAIREAR
                     SKDGGLQDAGITKFRPSQKGIDLTIDLCPSHRPLDRVVFTDLIHELGRIESPVPVGIS
                     VTGRWMNMHAGDLKWLDSLQQSGLLDIVWINHTYNHNVSKSAPVKKNFMLEPGTDING
                     EVLKTEVAMLENGILPSVFFRFPGLVSNSAIFDKILNYGLIPVGSDAWLAKGQYPEYG
                     SIVLIHANGNEPVGVRDFIRLLGQKQAEILSKRWELFDLRESIIDDETR"
     gene            6649..9267
                     /locus_tag="PKI35_09685"
     CDS             6649..9267
                     /locus_tag="PKI35_09685"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNX44695.1"
                     /translation="MPFYRPAVDFINARFTAIHENRKFIAQFLLAALFIFLGVWFVKH
                     EQAELGEVKDVLLSSRWKYILLGIILTGVYIVLQGVMYQMAFASVKNRVKLSTTMLLF
                     LKRNFISVFMPAGGVASLAFFTGEVEKQGVTKTRIHFASSIYAFTGILSVVLAAVPVF
                     VFAIIKGLTGSGEWISLAVIVAMVFFLFSVYHSIIHKKLIYRLIVKLLPSSEVFFEDI
                     SNRSLDVKYLVYTVLISILVDVTGVVHLLIAMKALGYQASLFYALLGYLTAVLSLIVS
                     PFMRGLGAVEVSLSFILIRLGYSNVEGVAIAFLYRFFEFWVPLLAGAFSFLVRINRLL
                     MRVFPALLIFALGVLNIISVLTPAIQERLLRLEQFVPIEVISASNYFVLLAGILMLLS
                     AIYMLKGLRNAWWIGLALSAFSVLGHLFKAIDYEEAGIALLVFMMLLFSRREYRIKGN
                     PRLHFIGIQSAMLAVVTVLIYGTIGFYFLDEKHFGINFSALQSVRYTIRNFLLLGSSD
                     LVPHSRFAKDFLLSINLSGAVSVSFLFYTMIRPYIFKGEAYPEGLARARQLINSYGRS
                     GMDYFKTYNDKLFFLPEGIDAFISYRVAGNFAVALEDPVAADEQTLEQCIGQFDKYCY
                     DNGLKSIFYRVPEESLRYYERYLKKNLFLGQEGIVKLESFTLQGERNKALRNAINKIS
                     DRGYSSTVHLPPVKDGLMQKLKAVSDEWLRANQRHEIVFSQGMFVWEELKQQTILTVE
                     NEEEKVIAFVNIIPDYAPGEGTYDLIRKTDDAPNGVLDFLLTELFRYLKSQGCSTVNL
                     GFAPLSGINDPNTFPEKSMRFAYEKIKAFSHYKGLRNFKEKFSPEWRNKYLVYSNDYD
                     LLQIPAVLTKVIKVAD"
     gene            9267..9923
                     /locus_tag="PKI35_09690"
     CDS             9267..9923
                     /locus_tag="PKI35_09690"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNX44696.1"
                     /translation="MGKKILIITGLIACVLACIGDFAVTFIFGRYYPGYSQLHDTMSS
                     LGASASPVSDKVSLWWVILGILIILFAIAFRLVIGPRKYSWLAAIMLVIYGVGEGFGS
                     GLFKADPATGALISASFIHNALGGTGVAALMFFPLVMQKIIPRSENVRLFRYSTAVFI
                     IGLIFIALFLFRFSNFEILKIYKGLWQRLFVLNLYIYLLVVDGILIGKFFRKHTGSRF
                     "
     gene            complement(10174..10596)
                     /locus_tag="PKI35_09695"
     CDS             complement(10174..10596)
                     /locus_tag="PKI35_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44697.1"
                     /translation="MPIEDYLLRQFEQMGRVLGLILARLMGLKNSGRVDEGIGFTNQT
                     LKDELDLDLDRLLEIPDNELVKYLLDEKKFGPANLEQLAEILLLIADGSDSGLIHNEL
                     RVKYYAKCLAILNYLEVSETTYSLDRHLKIERIKAISR"
     gene            complement(10888..11472)
                     /locus_tag="PKI35_09700"
     CDS             complement(10888..11472)
                     /locus_tag="PKI35_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44698.1"
                     /translation="MKKTLGILCMAAMVTMFGCKKDETEETKFDNKVFVQEIFNAGMM
                     WNAQGKALKSSYHIYIDVDNTVTGIEGGTIHVLGNVSGNMNIDDQTGAITGGTMLLGL
                     TETINDFTLTSDGKTFTINGAPYISLAGTFTLQPGGSTFGTASSMEIGGGYRVTGDGY
                     DQTVNVQLTIIINTNGTGGTVSGNINGVAVHYTL"
     gene            complement(11687..12157)
                     /locus_tag="PKI35_09705"
     CDS             complement(11687..12157)
                     /locus_tag="PKI35_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNX44699.1"
                     /translation="MKYICPLITVADIQRSRDFYEGVLGQKVKYDLGENVTFEGDFAI
                     HLQSHFSRLIDNKPIRQGGNNFELYFEFDDIDHLADRLKEQKVEFVHEAREQPWRQKV
                     VRVYDPDRHIIDIGESLEYLSYRLSNEGLTAEQVSVAVGIPVDFVRASIDRFSK"
     gene            complement(12203..>12670)
                     /locus_tag="PKI35_09710"
     CDS             complement(12203..>12670)
                     /locus_tag="PKI35_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44700.1"
                     /translation="ISNQVHLPKNHSTMVKLKIAGCLSALILALFVVTSCEEENGEFQ
                     GDFRDSLTGTYACLETFHYFDPVNDTIMNWSADTISDNTLVTIEKYQDSSLNIVIGNY
                     SFIATYEGDHKFTCLECNGPPDYADFFAPDSINIIRKSGVTNYSRYIGKKNAL"
BASE COUNT         3042 a         2871 c         3023 g         3734 t
ORIGIN      
        1 gtgttttgtg tttccccctt ctctttaacc cttgaccttt accctgccgt cttacgctca
       61 gcctgcccag gcaggtaaac tgttaactat tggctgttgg cgttttgcgt tttgtgtttt
      121 gcgttttgtg ttttgtgttt tccgttttcc gttctccgtt ctccgttctc cccttccttt
      181 cacacaatgc catacaccgg caggtaaagt tctccgtttc tttagggtat cgtcttaaac
      241 accgtatgtc tcagcacaat ttctacaccc agcattacca gcgctatcag aacaaacggg
      301 aagaactcct ctttcttgcg cctgaattcc gtcacatcta ttttggattt ttccatttta
      361 tcaatttcct ggtaaatgct ttcaagcttc cttttgttga cggccctgaa gtatttgccg
      421 tttgtcatcg aggatatttt ctgcagcagg gcttcatcta ttttaacttc cacattctga
      481 tactggatgc cgaagggcgt ctcgaaaggg tagggagctg ttcctatggt gccgatgccg
      541 atggtgtaaa tacgaatgcc gtatagtgag gcaatttccg ccgccgttac ggggtcaatg
      601 gcgcccatgt tattaatgcc gtcggtgagc aggattatta ctttgctgac ggctttgctt
      661 tcctttaacc tgctgacggc cgtagcgagg ccgtcaccca gggcggttcc gtcgtccacc
      721 atgccgcttt taatttcacc ataaaggttg atgagtacgg aatgatcggt ggtaagcggg
      781 cattgggtga agctttcccc gctgaaaact accagcccga tgcggtcgtt cgggcggcca
      841 ttgataaacc gggtggcgac atccttggca gcttcgaggc ggttgggcct gaaatcttcg
      901 gccagcatgc ttcccgaaat atcattggcc agaatgatat caataccttc aacgctgata
      961 ttctggctgc tcatgctccg ctggggacgt gccagggcaa tgatgataaa tatgagcgcc
     1021 agcatgcgca gagcaaacag caggtgcctg caccgtaccc tccagcctgg cttaaccgaa
     1081 gataatgccg taaaggtgga tacctgaaat tttgcatgaa ggtgcatcag cctgtaccaa
     1141 taccagcctg ctgctgctgg aacaagcaac aggagccaga agtaagcggg attggcaaat
     1201 tcaacgttca tcatcattca gtggtttgtt gggtcatcgc agtatctgac ggggtggttg
     1261 cctttgtttc ctgctggacg acgggcttgg tgcggtccac aaaaaggata gcatctttca
     1321 ggctctgttc attctcagga ggtaaaggaa tttctttggc aaactttaca aggtcggcaa
     1381 gccggagaac ccgggccagt tgttctgtgg actggcttac cgccggcgag ttgctgcaca
     1441 attccaggat atcgtcggtg gtcatttcca ccgcgggaat aaggtgctgg tactcaaggt
     1501 accggcggag gatatccgac aattcgctgt ggaaaagttt tatacggcct tcctgccagt
     1561 gtttcttttc cgaaagttct ttcagctcgt tgagcgctgt ttgccaggcc gggagagccg
     1621 gtttaacgat tttcgggatg aaaggtttgt tttgtttacg ccgccagagg taatagatga
     1681 caacggcggc cagcgccagg actgccagtg cgccaaggat atatggcaat agttcgccaa
     1741 atgtcagcct ttgtttcatc ggggctttga tatccctgat ggccagcgtt gtatccacgg
     1801 ctaccgtgct aacctgcatc atcagggagt cggtgtatga attgtatcct gccgtgtcgc
     1861 tgcctttacg gtaaataaaa ttcagcggag gtatggaatg aaatccggta tcgaatgaag
     1921 tgatggtgat atactgactg tactgtaaca ggtctctgcc cttaacagcc gatgtatcaa
     1981 ccggtccgcg cttcaccact tccaccgaag ggcttagggt gtcgggaata acaggccatt
     2041 gaactttaac cgatttggga gcggtaaagc caagttgcag ccttacctgt tcaccgatgc
     2101 gtatggcagc ggtatcaagt tttgcagcag ctttctgtgc ttttaccaca ggacatgccg
     2161 tcagcataac cgtcaagaca ataaggaatg caaccggtgt aaccgtttta ggatgatgcc
     2221 cagatcccat tgttatatat ttaatacctg gcttcacgtt ttttaaatag gccgatcaat
     2281 ggtttcacat aatcctgatc ggtgccgaac ctaacggtat caacaccgct gcgcataaat
     2341 atcccgttca gggtattttc atgatttttc caccaatgcg tatatgcctg cctgacctgc
     2401 aggtcggata aatcgaccca ggtactggca ttggattcct tgtcgtaaac ctgcatcagt
     2461 ccgacgggag gcaggtttgt ttccgactcg tcatacacct gcagtgcaac gatatcatgt
     2521 ttcccagagg caatctgcag cgctttctca aaatcatggt caaaaaaatc ggagatgatg
     2581 aaagccgtgc aacgtttctt gatggcgttg gtaaggaacc gcagcgcctc cgaaatatcg
     2641 gtcttctggc ttaccggcct gaagtccacc agttcgcgga tgatccggag tatgtgggaa
     2701 gtgccttttt taggtgggat aaatttctcg atgacgtcgc tgaagaagat caccccgacc
     2761 ttatcattgt tctgaatggc cgagaatgcc agtacggcgg ctatttcggt catctggtct
     2821 tccttgaaca tcttccgggt gccgaattca ttcgaaccgc tcacatcaat cagcagcatc
     2881 acggttagtt cgcgttcttc gtcgaaaacc ttaacaaagg gatggttgaa gcgggctgtt
     2941 acattccagt caatattccg gatatcatcg ccgaactgat attcccggac ttcgctgaaa
     3001 gccatacccc gtcctttgaa ggcgctgtgg tactgtcccg aaaaaatctg atgtgaaagg
     3061 ccacgggtct tgatctcgat cttacgcact ttcctgatga tgtccgaggt ttccatggtg
     3121 gatatggtta attacgggat ttaccggaat ttactgatag ctgcatcgta caatgataca
     3181 tgcgataaaa cggcagaatg cagattctag ggaacttcaa ccgtattgag tatttcactg
     3241 ataatctctt cggtggtgat gttttcggct tcggcttcat acgtcagccc gatacggtgg
     3301 cgcataacgt cgtgtgccac ggcgcggatg tcttcaggaa ttacatatcc gcggcgtttg
     3361 atgaaggcaa aagccttggc ggccagggcc aggtatatgc ttgccctggg cgatgcaccg
     3421 taacggatga ggctctcaaa atttttcagc ttataatcgg ccgggaatcg cgatgcaaag
     3481 acgatatcgg taatgtaatt ttcaattttt tcgtccaggt aaacctgccg caccacttca
     3541 cgcgctttca gaatgtcttc cggcttcagg atagtgttgg tcgaaagtgt ttcctggctg
     3601 atattctgcc ggagaatgag tttttcctcc tgcttttgcg ggtaagaaat aaccaccttg
     3661 agcataaaac ggtcgacttg tgcttcgggc agaggatagg tgccttcctg ttcgatggga
     3721 ttttgtgtgg ccagaacaag gaaaggttcg ggcagtttat aggtcttttc gccaatggtc
     3781 acctgtcgtt cctgcatggc ttcgagcaac gcactctgca ccttggcagg agaacggttg
     3841 atttcgtcgg caaggatgaa attagcgaaa acaggccctt tccgtaccat gaagtcttca
     3901 ttcttctggc tgtagatcag cgttccgata aggtcggcag gtaaaaggtc cggagtgaac
     3961 tggatgcggc tgaaattcag gtcgatagtc cgggctaatg atttgatggc gagcgttttt
     4021 gccagaccgg gaacgccttc cagcaggatg tgcccgtcgg ccagtaaacc aatcagtaac
     4081 ctttcaatca tctgtttctg tccgacaata acacgatgca tttccatcgt gagcatatca
     4141 acaaaggcgc tctcagtttg aatcctgtcg ttcagttctt tgatgttagt ctctaccatg
     4201 ataagtttag gttttacagg gcaaaattag gaacaggcat cacatcctta gtgcagtacg
     4261 ctgttaaaaa ttgttaaacc ggaatgattg gatttgcggg aattatagtg tttccaggct
     4321 ttattccgga taatagttgt gcatgaatta aacatgtgac gaattgtata ttttacacct
     4381 tgagtttgct gttcatggaa tgatgagggg cgccggatct acaggacgat aggcgaacgg
     4441 tatatgtggt ttggatcaaa aagcgaaagg ctgccgggct cctgcgcttt ttcagcctga
     4501 aattttcttt accggcgaac cggcattgtt tgtaacaatc gatactatct cattcatcat
     4561 cgaaacctgc aatttctgaa gttcaaagag ttcctgttgc tgatccatga tgaagtgatc
     4621 aactttttcg tgtaaagacc tgatctccag ttctgatttc aggttaatca tataatcgtt
     4681 tttggcccgt tcccggtctt tttcttcctg ccggttttgg ctcatcatga tgaccggggc
     4741 ttgaattgat gctacacatg aaagtatcag gttcagcagg atgaaaggat aaggatcgaa
     4801 acctgtattg gccagccagt atatgttgat ggcgatccag acgaggatga aaaccatgaa
     4861 agcgatgatg aatttccagc ttccgccgaa agtagccact ttatcggcta ttttctggcc
     4921 cgccgttagt ttcggctgcc tctcgctgtc aagtttgtcg gacagtgttg cattggctga
     4981 catagaattc agaacggttt tttcaggttc atccaatttg ccgacttcac gggtcaggta
     5041 attggttatg tatcgttgct ggtattcgtt taattcgccg atcgaaataa aactgttatg
     5101 gttaatggcc ggattctcct tctgcatgag atgcagtacc gggtggcgga tggtttttgc
     5161 cgaaaccctt tccataaccg gaaattcctg atgcgacaga tcgcttatga atgtatccat
     5221 ggcttaaata tatatattgt ctggttgatt acctggtttc atcatcaata atgctttccc
     5281 tcaaatcgaa aagttcccat cgtttcgaga gtatttccgc ctgcttttga cctaataacc
     5341 tgataaagtc gcgaacgccg accggttcat ttccgtttgc atggatgagc acgatgctgc
     5401 cgtattccgg gtactgccct tttgccagcc aggcatcact gcccactggt atcagcccgt
     5461 aattcaatat tttatcgaag attgcgctat tggacaccag gccggggaac cggaaaaata
     5521 ccgaaggaag gatgccattt tcgagcatgg cgacttccgt ttttagtact tctccattga
     5581 tatcggtgcc gggttcaagc ataaagtttt ttttaacggg agctgatttt gagacattat
     5641 ggttgtatgt atggttgatc cacactatat caagcaaacc cgattgctga agactatcga
     5701 gccacttcag atccccggca tgcatattca tccatcttcc tgtaactgaa atgcctaccg
     5761 gaacaggact ttcaatcctg ccgagttcat ggatgagatc ggtgaacaca acacggtcga
     5821 gcgggcgatg tgacggacaa aggtcaatgg taaggtcaat ccctttttgc gaaggcctga
     5881 attttgtgat acctgcatcc tgcaaccctc cgtctttact cctggcttcc cttatcgctg
     5941 cgatataggg cgtatttcgg aacctggaac tgaccgtttg ccatgatcct gagattacct
     6001 taatgctgtc ggcatttata acccgggtta gaagagtctc cggcgataca gtgagataga
     6061 atttctgatt atctctctta aactgcctga tcacgatcac tttttcggaa ttccttcgca
     6121 gggttcccca acctgtaaaa agctgataat cggtaacagg ttttgaatta cccggattgc
     6181 atgttaacat catccaggat aatatgatca gggtttcaaa tttcatttca tccgtgaatt
     6241 attatgatta tcaggggctg atggacttcc atgccggata ttttactttc ggtcagatcg
     6301 ttttgccagg ttcacaggcc tttaggttca ttcgcaacca tgtttgtttc ataatgttgg
     6361 catatgacaa acatctttct caccaggcgc ttcggtgaac cggatcagcg acgttatcgc
     6421 cattctaaaa tacgacaaaa ttccatgttg cgcgctttgg tccttcgtca atttgccggg
     6481 ccttcagata atgatgataa acgataagga atgattgata ttatccgatg aatttaacat
     6541 caagagctgt ttcctgctcc tgtaattcaa atcatggctg gtaattttct gtgaatcaaa
     6601 aaggtatttt ttgcgtaagt ttgtataaag agtgatttcc tgaatcccat gccgttttat
     6661 agaccagccg ttgattttat aaatgccagg ttcactgcga ttcatgagaa ccggaaattt
     6721 atcgctcaat ttttactggc ggctctgttt atttttcttg gggtctggtt tgtaaagcat
     6781 gagcaggcgg aactgggtga agtcaaagat gtattattat catcgcgatg gaagtatatc
     6841 ctgttaggga ttatacttac aggcgtctat attgttttac agggggtaat gtatcagatg
     6901 gctttcgctt cggtgaaaaa cagggtaaaa ctttcaacga cgatgctgtt gtttctgaaa
     6961 cgcaacttta tcagtgtttt catgcccgcc ggaggtgtcg cttcgctggc tttttttacg
     7021 ggagaagttg aaaaacaggg agtaacaaag acgcggatac attttgcatc atccatttat
     7081 gcctttacag ggattctgtc ggtggttttg gctgccgttc ctgttttcgt ctttgccatc
     7141 atcaaaggcc ttactggttc cggagaatgg atttccctcg ctgttatcgt ggcgatggta
     7201 tttttccttt tttctgttta tcattccatc atacataaaa aattgatcta ccggctgatc
     7261 gtaaagttgt tgccctcttc cgaagttttt tttgaagata tttccaaccg cagcctcgac
     7321 gttaaatacc tggtatatac cgtattgatt tcaatccttg tagatgtaac cggcgtcgtt
     7381 catcttttaa ttgctatgaa agcccttggt tatcaggctt cactctttta tgctttgctc
     7441 ggatacctga ccgccgttct ttccctgata gtctcgcctt tcatgagagg gcttggcgcg
     7501 gtagaagttt ctttatcgtt tatcctgatc aggttgggct attcaaatgt ggaaggagtc
     7561 gccattgctt ttctctaccg tttcttcgaa ttttgggtac ccttgctggc cggggctttt
     7621 tcttttctgg tcagaataaa ccggttactg atgcgggttt tcccggcatt gctgatattt
     7681 gccctgggag tcctgaatat catttccgtt cttactcctg ctatccagga acgtctcctc
     7741 aggcttgaac aatttgtacc gattgaagtg atcagcgctt cgaattattt cgtccttctg
     7801 gcaggcatcc ttatgctgtt gtcagcgatt tatatgctga aggggttacg aaatgcctgg
     7861 tggataggcc ttgcgctctc agctttttcg gttttggggc atcttttcaa agccattgat
     7921 tatgaagaag caggtatagc gctgttggtt tttatgatgc tccttttttc aagaagggaa
     7981 tatcgcatca aaggcaaccc ccggcttcat ttcatcggga tacagtcggc aatgctggcg
     8041 gtggtgacgg ttctgatata tgggaccata ggcttttact tccttgatga aaagcatttc
     8101 gggataaatt tcagcgcgtt acagagtgtc cgttatacca tcaggaactt cctgctcctg
     8161 ggaagttccg acctggttcc gcacagccgc tttgccaaag attttcttct cagcataaat
     8221 cttagcgggg cagtatcggt ttccttcctg ttttatacca tgatcaggcc ctatattttt
     8281 aaaggagaag cataccctga aggcctggcc agggccagac aattgataaa ttcctatggc
     8341 cgttccggaa tggattactt taaaacatac aatgataaac tgttttttct tcccgaagga
     8401 attgatgcat ttatatctta ccgcgtggcc ggcaattttg cggttgcgct tgaagaccct
     8461 gttgctgctg atgaacagac tctggagcag tgcatcggac aattcgataa gtactgctat
     8521 gataatggcc tgaaaagcat tttctatcgg gtcccggaag aaagcctgcg gtattatgaa
     8581 cgctatctga agaagaacct gttcctcggt caggaaggca tcgtaaagct ggaatctttt
     8641 accctgcaag gtgaaagaaa taaggccctt cgcaatgcca ttaacaaaat cagcgaccgg
     8701 ggctattcct ccaccgttca cctgccgcct gtaaaagacg gactgatgca aaaactgaag
     8761 gctgtatcgg atgagtggtt gcgggccaat cagcgacatg agatagtatt ctcgcagggt
     8821 atgtttgtgt gggaggaatt gaaacagcaa accattctta ccgttgaaaa tgaagaagaa
     8881 aaagtgatag cctttgtcaa catcattccc gattatgcac ccggtgaagg gacctatgac
     8941 cttatcagaa agacggatga tgctccaaac ggggttctcg attttttgct gacagagttg
     9001 ttccgatacc tgaaatcgca gggctgctca acggtcaatc tgggttttgc cccgctgagc
     9061 ggaataaatg acccgaatac attcccggaa aaatcaatgc ggtttgccta cgaaaaaata
     9121 aaagcctttt cacattacaa aggccttcgc aatttcaagg aaaaattctc tcctgaatgg
     9181 cgcaacaaat accttgttta ttcaaacgat tatgacctct tacagatacc ggcagttttg
     9241 acaaaagtta ttaaagtagc ggattgatgg gaaagaaaat actgatcatc accgggctga
     9301 ttgcctgtgt cctggcctgc attggcgatt ttgccgtcac tttcatcttc ggcaggtatt
     9361 atcccggata cagccagctt catgatacaa tgagctcatt gggggcgtca gccagccctg
     9421 tttctgataa ggtgtccttg tggtgggtca tcctgggaat cctgataatt ttatttgcta
     9481 tagcattccg actggttatt ggtccccgga aatattcctg gttagccgcc attatgctgg
     9541 tcatatacgg tgtgggcgaa ggattcggtt cgggattgtt caaagccgat ccggcaaccg
     9601 gggcgctgat cagcgcatca tttattcata atgctcttgg cggtacaggc gttgccgcac
     9661 tgatgttctt tcccctggta atgcagaaaa taattccccg ttccgaaaac gtgcggcttt
     9721 tccgctattc aacggctgtt tttattatcg ggctgatttt catcgccctc ttcctttttc
     9781 ggttcagtaa ttttgaaatc ctgaaaatat acaaggggct ctggcagagg ctttttgtcc
     9841 tgaacctgta tatctatctc cttgttgttg atgggattct gatcggaaaa ttttttcgaa
     9901 agcataccgg cagccggttt taagaaatcc cgaataatcg gcaacagagc aggcatggct
     9961 atcgttgaaa cttgcttgtg acaccctttt gaccggaagc ggattttgag atccggggct
    10021 tgaaaaataa agcaatcgct tatggttcta aattatagtc aggcagtata cagagaccgg
    10081 taatagtgcg ctttgcacaa aaccccttaa actgccgata tccgtcataa gtgaaaccat
    10141 aacaaacaaa aaggaaggcc ccgaccggat aatttatcgg gatatagctt ttattctttc
    10201 aatcttgaga tgacgatcga gggagtaggt tgtttccgac acttcaaggt aattcaatat
    10261 ggccaggcat tttgcataat actttacccg taattcatta tgaatcaagc ccgaatcgga
    10321 tccgtcggcg atcaggagca ggatttcggc cagttgttcc aggtttgcag gaccgaactt
    10381 cttttcatca agcaggtatt tcaccagctc gttgtcggga atttcgagga gacggtcaag
    10441 gtcgaggtcc aattcgtcct tcagcgtctg gttcgtaaat ccgatgcctt catccacccg
    10501 gccgctgttt ttcagtccca taagccgggc caggatcagg cccagtaccc ggcccatttg
    10561 ctcaaattga cgcagaagat agtcttcgat cggcatagct ggtggcatta aaaggcaata
    10621 aaggtaacgt aaaagggttt gcgccgggca tgttccgcaa ttccggtttt gccgggctgg
    10681 cattgaaaag cgatttccat acagcgattc ccaattcggg aaccttatat agggactatc
    10741 atggttccgg ataagtatct gccggttgaa ctgcaggatg cattgcattg gataagcatg
    10801 aaccccggtg tcctgacggt catcggggtt caataaattt aagggccggc ataaaccgcc
    10861 ggtaaaagaa ggccgggagc tatgtggtta taatgtataa tgaacggcca ctccgttaat
    10921 gttgccgctc accgtgccgc ccgtgccatt cgtattgatg atgatcgtca actgtacatt
    10981 caccgtttgg tcataaccgt cgccggttac acggtagccg ccgcctattt ccatactgga
    11041 ggcagtcccg aaagtgctcc cgcccggttg cagggtgaag gttccagcaa gcgagatata
    11101 gggcgcgccg ttgatggtaa aggttttgcc gtccgatgtg agcgtgaaat cgttgatggt
    11161 ctccgtcagt cccagcagca tcgtaccgcc tgtaatagcg cccgtctgat cgtcaatgtt
    11221 catgttccct gaaacgttac ccaggacgtg aatcgtaccg ccttcaattc cggttaccgt
    11281 attatccaca tctatataaa tatgatatga cgattttaaa gctttaccct gcgcattcca
    11341 catcatgcct gcgttgaata tttcctgaac gaagacctta ttgtcgaatt tggtctcttc
    11401 ggtttcgtcc tttttacaac cgaacatggt taccatggca gccatacaga gaattccaag
    11461 tgtttttttc attttttttg cgtcagtgtt ttgagtgcta actccgcact tttagaattg
    11521 gatagggttg gggaataatg attcaggata ttaaccggtt taatctggtt cgttgttgat
    11581 tacattaaca aatgtataaa atttgttaca ttatcgtcat agtccggaaa aaaaacttcc
    11641 tgattttggt tcaggttgat ggagcctgtt ttaaagttac aggcgtttat ttgctgaatc
    11701 tgtcaatgga ggccctgaca aaatcaaccg gtatcccgac ggcaacggaa acctgttctg
    11761 ccgttaatcc ttcgttgctc agcctgtacg acagatattc cagcgattcg ccgatatcaa
    11821 tgatgtgcct gtccggatca tacactctga cgaccttttg cctccagggt tgttccctgg
    11881 cttcgtgtac aaactcaacc ttctgctcct ttaaccggtc ggccagatga tcaatatcat
    11941 caaattcaaa gtatagttca aaattattgc ctccctgccg gatcggcttg ttatcaatga
    12001 gccggctgaa atgcgattgc aaatggatgg caaaatcccc ctcgaatgtg acattctctc
    12061 ccaggtcgta ttttactttc tggccaagga cgccttcata gaaatcgcgg gaacgttgga
    12121 tatcggcaac ggttattaac ggacaaatgt acttcatggc tgattatttc tgaaaatgat
    12181 gaaaaggttt ggtgatgatt cgttataatg cgtttttttt cccaatgtac ctgctgtaat
    12241 tggtcactcc tgatttgcgg ataatattaa tgctgtcggg agcgaaaaaa tcagcataat
    12301 caggagggcc gttacattcg agacaggtaa atttatgatc cccttcatag gttgctatga
    12361 aactgtagtt cccgataacg atgtttaacg aactatcctg atatttctca atggttacca
    12421 aggtgttgtc cgaaatggtg tcggcgctcc agttcatgat cgtatcgtta accgggtcga
    12481 aataatgaaa ggtttccagg caggcgtatg tcccggttaa actatcccga aaatcaccct
    12541 gaaattcacc gttctcctcc tcacaagaag ttacgacgaa cagggcaagg atgagggctg
    12601 acaggcagcc ggcaattttc aacttgacca tggttgaatg attcttgggc agatgaacct
    12661 gattgctaat
//
LOCUS       DAOPYV010000077        12481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_5652_length_12481_cov_12.342508, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000077 DAOPYV010000000
VERSION     DAOPYV010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12481
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5652_length_12481_cov_12.342508"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2719
                     /locus_tag="PKI35_09715"
     CDS             <1..2719
                     /locus_tag="PKI35_09715"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX44701.1"
                     /translation="QVTLVATNAAGCSDSAQLTVTISPKPGVDFYTGTIMCEGSPVEF
                     YTDTTVTGIATVQSYDWDFGDGTAHSTLQNPVHTYTIQGTYPVTLTIVDVSGCQNSVS
                     HSVTITLGPVSAFTTEQSCAGTATQFTDLSSAPSGSTIVSWHWDFGVTGATDDTSNMQ
                     NPVYTYSQPGLYTVSLTTTTQNGCSNTVMQPVQIWNTPTAAFAYNTTPCSNGTVQFMD
                     QSAAFQATVTGWLWEFEPYQYSTLQHPSYTYFKTDTCYAVKLIITDSRGCTDTVIQQV
                     CVPAAFAVDFTQQNTCAGSAVQFTSHLISPADDEINSYLWNFGDPASGTANTSAAQNP
                     GHIFQQAGYYTVTLTANDIYGCTDTYIQTVQITGLPQASFTWQEGECDSTITFTSTSL
                     GIAPIASYTWSFGDGTTQTLTAPANTVKHKYLQGGEYTVTLTVTDENGCSHSAVQTAE
                     LTSCLVAAMMIPDTLLCQNYVITFTDRSSGGAGITGWSWNWGDGTAPAQYNVYTPSVT
                     HVYTAPGTYWVSLKITATVNGSVVSDSTATKITVYSTPLAGFVTDNVCLGDKTAFTDT
                     TNVNGALWVSYAWNFGDPYSTNDISEDRNPTYTYGNVGEFDASLIVTNQFGCTDTASN
                     PVAIHGLPDAAYEYSLACQGKPTYFFDHSEPFISPLTHRGWVISEDKQIGWMEGTTPS
                     FTFDEIGDYNVLLTVADTNGCIDTVSHHVTVYPTPVSAFGIEKDFDNVQGQARFINGS
                     LNADKYYWDFGNGETSSAESPVVHYDLDGDYLVQLYAYNQYECVDSTSMLYRLMYTGL
                     WAPNAFAPSGPIEATRLWKPVGINLSYYRAEVYDRWDALIWSSEKLDEKGSPVEGWDG
                     TYHQVPCQQGIYVWKITAIFRDGKIWRNNDTGDHTDMPRQTSGTITLIR"
     gene            2876..4306
                     /locus_tag="PKI35_09720"
     CDS             2876..4306
                     /locus_tag="PKI35_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX44702.1"
                     /translation="MNKKNTSKGRRDFLKLTAMGTVAASLAPLSGAFASPSGNEAEIL
                     PAPPAPQAPKPGRKSVMGLACEPIETVRIGIIGLGMRGSDAVRRLLQIEGVKVMALCD
                     VLPERVKESNETVTGAGQEAAIEFSGEEGWKKVCEREDIDLIYTCTPWYLHTPIAVYA
                     MKNGKHIATEVPAAMTLNECWQLVNTAEETQRHCMMLENCCYDFFELATLNMARDGIF
                     GEILHGEGAYIHDLRWLKFAAENKGGYYHQWRLEYSKKHNGNLYPTHGLGPVAQIMGI
                     NRGDRLDFLTSMSTKQAGLSLYAAGKFGAGSPEALQEYKLGDMNITMLHTIKGHTIMI
                     QHDTTSPRPYSRIHMISGTKGMAVKYPEQNIALEPSAHEFLKKEDLDALLKKYEHPLA
                     QQIGEKARKVGGHGGMDFIMDYRLIYCLRKGLPLDQDVYDAATWSCIVPLSEISVEKK
                     SRSIEIPDFTRGAWQTAQPWPVVTIE"
     gene            complement(4416..5174)
                     /locus_tag="PKI35_09725"
     CDS             complement(4416..5174)
                     /locus_tag="PKI35_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX44703.1"
                     /translation="MKTCRTIFENELKSLRKTILIFLFMIAVMPAAMSQRGGSFYFEP
                     SVALTSRWIVNQAIYGNGELPYVTSFGLYGGFRTYYFASSDVGFNYGIGYRRMGQNYE
                     GEESNANATRKVKLDYIQIPLMAMFALADREYPTWFSVGPQFCFLAKANQYFTREEGG
                     NPLNNPNYLPEGDTNVFQWYKPMDVMIVMEFNKMFKFSKAPRLSMNYTIESAFGIMDI
                     NDDAYRIPNFREIYSPSRNMYFGFRVGIMYKAVR"
     gene            complement(5188..5733)
                     /locus_tag="PKI35_09730"
     CDS             complement(5188..5733)
                     /locus_tag="PKI35_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44704.1"
                     /translation="MPNMLMIAGNSRNIGKTALACAVIERFSAFQKIYGLKVTSIRPG
                     EQGFHGIHDGLSEELFSIMEEKDPSSRKDTSKMLTAGAEKAYYIRVADTEMPMAAKYL
                     EGFLPENEPVVCESRSLRKEVKPGLFVFLWADKVDSAKIDIEEYVRIADVVIRYIPGE
                     GNTAKMAGKINLENNRWIYIN"
     gene            complement(5840..6205)
                     /locus_tag="PKI35_09735"
     CDS             complement(5840..6205)
                     /locus_tag="PKI35_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNX44705.1"
                     /translation="MKKYENVRPEYKIWFSTPEGEGILGDGKWKILMKIDECGSLVKA
                     CEEMGITYRRTWNDLKKVEKLLGFDLLETSRGGQEGGSTRLTAEGHKLVVAFRRFHEK
                     MDRLMAEEFRSLLEELIKE"
     gene            complement(6409..7398)
                     /gene="obgE"
                     /locus_tag="PKI35_09740"
     CDS             complement(6409..7398)
                     /gene="obgE"
                     /locus_tag="PKI35_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNX44706.1"
                     /translation="MAASNFVDYVKIFLRSGNGGAGSAHFLRTKLKPRGGPDGGDGGR
                     GGHIIIKGNSQLWTLLHLKYTKHVMAENGTGGSGALKKGADGGDIIIEVPLGTIVKDA
                     ETGEPETEITFHGEEKIVMRGGRGGLGNDHFKTAVNQAPKYAQPGEPGIEGWKVFELK
                     VLADVGLVGFPNAGKSTLLSVLSAAKPEIADYPFTTLRPNLGIVSYRDGLSFVMADIP
                     GIIEGASEGRGLGLRFLRHIERNSVLLFMIPADSKDIKKEYKILLNELKQYNPELLDK
                     ERVLAITKCDLLDEEMMSLIRKELPRVPAIFISSHTRQGLTALKDSLWKSLNG"
     gene            complement(7781..8950)
                     /locus_tag="PKI35_09745"
     CDS             complement(7781..8950)
                     /locus_tag="PKI35_09745"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNX44707.1"
                     /translation="MLNIALFGPPGAGKGTQSQLIIEKYNLVYISTGDLLRQEIAEET
                     ELGNRVKDIIRRGELVSDEIMVELIEKKIVSSNDSNGILFDGFPRTFVQAYILDGLLL
                     KLNTSLSCMLSLEVPNEELLQRLLERGKISGRSDDTEDVIRHRLVEYESKTLPVKEYY
                     RERNLYYPIDGVGPIPEIFERLNAQIELSLQKEWFNLVLSGSPGSGKGTQGKLLARKF
                     NLYYISTGALLRKEVLNRTEVGLKVQEKMAQGELVPDEVVIPLIEQEMKRHEDVNGFI
                     FKGFPRTILQAYILDGLLRRLNMRVSCVVEMEVPTLDLIRRLDKRGRSEKARPYDQST
                     EMIIRRLEEYETKTKPSLEVYRKQGKLLEMDATGSREEVIDRLSAAVASVMKKKR"
     gene            complement(9034..9573)
                     /gene="hpt"
                     /locus_tag="PKI35_09750"
     CDS             complement(9034..9573)
                     /gene="hpt"
                     /locus_tag="PKI35_09750"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNX44708.1"
                     /translation="MADTWKIHDKTFTKYMPDRKIQAGVRKLAARLNKEYEGKKPLFV
                     AVLNGSFIFASDLLKRITIDCEIAFVKVSSYRGVNSTGKIHELIGLEIPVEGRHVILV
                     EDIVDTGLTIIKVIEKIKSMNAAEVKVAACLMKPDAYKKDYPIDYVCFRIPNDFVVGY
                     GLDYDGLGRNSADIYRIEE"
     gene            9932..10438
                     /gene="purE"
                     /locus_tag="PKI35_09755"
     CDS             9932..10438
                     /gene="purE"
                     /locus_tag="PKI35_09755"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNX44709.1"
                     /translation="MKAIVSIIMGSTSDLPVMESAANVLNELKIPFEMNALSAHRTPE
                     KVEAFAVNAAGRGIRVIIAGAGMAAHLPGVIAAFTPIPVIGVPIKSTFDGLDALLAIV
                     QMPPGIPVATVAVNGAQNSAILAAQILATGDKELYDRVLKYKENLKKKIAEANEQLKD
                     VRFEFKVN"
     gene            complement(10458..11144)
                     /locus_tag="PKI35_09760"
     CDS             complement(10458..11144)
                     /locus_tag="PKI35_09760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HNX44710.1"
                     /translation="MAGNNKEAFSEDEMSGGIISRALGEYAGAHTSPEPEVLKQLSRE
                     THLKTTAPQMLSGHLQGAFLRMISRMLKPELVLEIGTFTGYSAICLAEGIRENGMLHT
                     VECNPEMEDIARRYFSLAGLSEKIILHIGDAFEIVEKIEGPFDLVFIDANKDDYIRYF
                     DLVFGKVAGGGFIIADNVLWYGRVTDKNASVDRETRSIVAFNEYIQHHNGVENLLLPV
                     RDGLMIMRKL"
     gene            complement(11131..12369)
                     /locus_tag="PKI35_09765"
     CDS             complement(11131..12369)
                     /locus_tag="PKI35_09765"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNX44711.1"
                     /translation="MSTPYQIHQFTNGIRLVHRRTGSPVAHAGVVVNAGARDEKPEEQ
                     GMAHFIEHLIFKGTQRRTARQVLDRIENVGADLNAYTTKEETTVYASFLSAYYSRTLE
                     LFADILFNSTFPEAEMKKEKDVVIDELNSYKDSPMEWIQDEFDGLIFGDHPLGKNILG
                     TPAHLKRFGREQAFDFTRRLYSTNQIVISSVGSIPFDKLVSLTEKYFGRQPVREGSPE
                     RLMPPEYCKQEMSRKYSKHQSHIIIGNRAYSYHDNRRYALALLSNMLGGPAMNSVLNL
                     VLREKHGIAYNLESNYQPLSDTGVFTIYLGTDTKMVEKAISLVTGELKKLRESKLSGN
                     RLKISKEQLKGQVAISVEAHQNEMLSIGKSLVVFNKVDTISEMIGKVDAISDMDVLEV
                     ANEILDPDKLSTLIFTNGRK"
BASE COUNT         3111 a         3259 c         2912 g         3199 t
ORIGIN      
        1 ccaggttacc cttgtcgcca ccaatgccgc cggttgctcc gacagcgctc aactaacggt
       61 gaccatcagt ccgaagccgg gtgtcgactt ctacacgggt acgataatgt gcgaagggag
      121 cccggttgag ttctataccg atacaacggt taccggcatc gccacagtcc agtcgtacga
      181 ctgggatttc ggtgacggca ctgcgcacag caccttgcag aatccggtgc atacctatac
      241 tattcagggc acatacccgg ttacgctgac cattgttgat gtttcaggct gccagaattc
      301 agtttcgcac agtgtaacca tcacccttgg ccctgtttcg gcattcacta ctgaacaatc
      361 ctgcgcaggc acagcaacgc agttcaccga tctttcatcg gcgccttcgg gaagcaccat
      421 agtaagctgg cactgggatt tcggtgttac cggtgcaacc gatgacactt caaacatgca
      481 gaatccggtt tatacctaca gtcagcccgg gctttatacg gtgagcctca ccacgacaac
      541 gcagaacgga tgcagcaata ccgtgatgca acccgttcag atctggaata ccccgacggc
      601 agcctttgca tacaacacga cgccgtgcag caacggaacc gttcagttca tggatcagtc
      661 agcggcattc caggccaccg tcaccggatg gctgtgggaa tttgagcctt atcagtacag
      721 cacactgcag catccttcgt acacttattt caagaccgac acctgttatg ctgtaaaact
      781 catcatcacc gacagcaggg gatgcaccga caccgtgatt cagcaggttt gcgtaccggc
      841 cgcctttgcc gttgatttca cgcagcagaa tacctgcgcc ggaagcgccg tacaatttac
      901 ttctcatctg atatccccgg ccgacgacga aataaattca tacctgtgga acttcggcga
      961 cccggcttcc ggaacggcaa acacctctgc agcccagaat cccggacata tcttccagca
     1021 ggcaggctac tacaccgtaa cgctcacggc caatgacata tacggctgca ccgacacata
     1081 cattcagacg gttcagataa ccggcctgcc ccaagcttcg tttacatggc aggaaggcga
     1141 atgcgacagc accatcacct ttaccagcac cagccttggt atcgcgccta tcgcttccta
     1201 tacctggtcg ttcggcgacg ggacaacgca aaccctgacg gctcctgcca acaccgtgaa
     1261 gcataaatac ctccagggcg gagagtatac cgtaacatta accgtcaccg acgaaaacgg
     1321 gtgcagccat tcggccgttc aaaccgcaga actcacctcc tgcctggtcg ctgccatgat
     1381 gattccggat acgctgcttt gccagaatta cgtaattacc ttcaccgacc gcagttcagg
     1441 cggcgccggt atcaccggat ggagctggaa ctggggcgac ggaacggctc ctgcccaata
     1501 caatgtttac acgccttcgg taacgcatgt gtacaccgcg ccgggaacct actgggtatc
     1561 gctgaaaatc accgctacgg tcaacggaag cgttgtttcc gattcaacgg caacaaagat
     1621 taccgtatat tcaaccccgc tggcaggttt cgttactgac aacgtctgcc tgggcgacaa
     1681 gactgccttt accgacacca caaacgtcaa cggggcgctc tgggtaagct atgcatggaa
     1741 tttcggcgat ccatacagca caaacgacat ttccgaagac cgtaatccta cctatactta
     1801 tggtaatgtc ggcgaattcg atgccagcct gattgtcacc aaccagttcg gctgtaccga
     1861 caccgcctca aaccctgttg ccatccacgg tttgcctgat gcagcctacg aatacagcct
     1921 tgcctgccag ggaaaaccga cttacttctt cgatcattcc gagcccttca tctcgccgct
     1981 tactcaccgg ggatgggtga tcagcgaaga caagcagatc ggctggatgg aaggaacaac
     2041 gccttcgttc accttcgatg agatcgggga ttataacgta ctgttaacgg ttgccgatac
     2101 caacggatgt attgatacgg tttcgcacca tgttacggta tatccgactc ctgtcagcgc
     2161 cttcgggatc gaaaaagact tcgacaacgt tcaggggcag gcccggttta tcaatggttc
     2221 gctgaatgcc gataaatact attgggattt cggcaacggc gaaacatcct cagccgaatc
     2281 accggttgtt cattatgacc ttgacggcga ttacctggtg caattgtacg cgtacaacca
     2341 gtatgaatgc gttgactcaa ccagcatgct ttacaggctg atgtataccg gtctgtgggc
     2401 gcccaatgct tttgcgcctt cgggaccgat tgaagccaca cgcctgtgga agcccgttgg
     2461 tattaatctt tcctattaca gggcagaagt ttacgatcgc tgggatgccc tgatctggtc
     2521 gtcggagaag cttgatgaga aaggctcgcc tgtggaagga tgggatggaa cttaccatca
     2581 ggtgccctgc cagcagggaa tctatgtatg gaagataaca gccattttcc gcgatggaaa
     2641 gatctggcgc aacaacgata ccggcgacca taccgatatg ccgcgtcaga ccagcggaac
     2701 cataaccctg atacggtaac gggcgttgtc tgtcgcctgc cttgccgtaa aatcagccct
     2761 tgagggtttc attctcctgc atgtgccggg acgaaaatac catgcaggaa ttaccggatc
     2821 tgttttgcga tcaacgctca ttcattactt ttgccggtaa acaaactgac attctatgaa
     2881 caagaagaat accagcaaag gccgccggga tttcctgaaa cttacggcca tgggtactgt
     2941 tgcagcaagc ctggctccct tgtcgggcgc tttcgcttcc ccatccggaa atgaagctga
     3001 aatcctgccg gcgcctccgg cgccgcaagc cccgaaaccg ggcagaaaat cggtgatggg
     3061 cctggcctgt gaaccaattg aaaccgtcag gatcgggata atcggcctgg gaatgcgcgg
     3121 cagcgatgcc gtccgccgac tgttgcagat tgaaggcgtt aaggtcatgg ccttatgcga
     3181 tgtgctcccc gaaagagtta aggaaagcaa tgaaacggtt acgggcgccg gtcaggaagc
     3241 cgctattgag ttttcgggcg aggaaggatg gaaaaaggtt tgcgaacgcg aggatattga
     3301 cctgatttat acctgtaccc cctggtacct gcataccccg attgccgtgt atgccatgaa
     3361 aaacggcaaa catatcgcca cagaagttcc ggccgccatg accctcaacg agtgctggca
     3421 actggtaaac acggccgaag aaacgcaacg ccattgcatg atgctcgaaa actgctgtta
     3481 tgactttttt gaactggcga ccctgaatat ggcccgggac ggcatcttcg gcgaaattct
     3541 tcatggcgag ggagcctata tccatgacct gcgctggctg aaatttgccg ccgaaaacaa
     3601 gggagggtac tatcaccagt ggcgccttga atacagcaaa aagcacaatg gcaacctgta
     3661 ccccactcac ggacttggcc cggttgcaca gattatgggt ataaaccgcg gcgacaggct
     3721 cgactttctg acttcgatgt cgacaaagca ggcgggatta agcttgtatg ccgccggaaa
     3781 attcggggca ggatcaccgg aagcgctgca ggaatacaaa ctgggcgata tgaatattac
     3841 catgctccat acaatcaagg gacacaccat catgatccag cacgacacca ccagcccgag
     3901 gccatacagc cggattcaca tgatcagcgg gacgaaagga atggctgtaa agtatccgga
     3961 acaaaatatc gccctggaac cttccgcaca tgaattcctt aagaaagaag acctggatgc
     4021 cttactaaaa aaatatgaac acccgttagc gcaacagata ggtgaaaaag ccaggaaagt
     4081 tggcgggcac ggcggaatgg attttatcat ggactacagg ctcatatact gcctgcgcaa
     4141 agggctgcct ttggaccagg atgtttatga tgccgctacc tggtcgtgta tcgtaccgct
     4201 gagtgaaata tcggttgaaa aaaagagccg gagtatcgaa atccccgatt ttacaagagg
     4261 agcctggcaa acagcccagc cctggccggt agtcactatc gaataaacgc ccctaccggg
     4321 gttgtccgtc gtcatgggtt cccggtttgg aaaattcgta aaacaaagag gccctgatat
     4381 cacataccag ggcctctttt taatatttct tccggctacc ggacagcctt atacatgatg
     4441 ccgacacgga aaccgaagta catgttgcgt gaaggggaat agatttccct gaaattcgga
     4501 atcctgtatg cgtcatcatt gatatccatg atgccgaagg ccgattcgat ggtataattc
     4561 atgctgagac gcggcgcttt ggagaattta aacatcttgt tgaattccat gacaatcata
     4621 acatccatcg gtttatacca ttgaaacaca ttggtatcgc cttcaggaag gtaattgggg
     4681 ttattgaggg ggtttccgcc ttcttcccgt gtaaaatact gattggcttt tgccagaaaa
     4741 caaaactgcg gccccacgga aaaccaggtc ggatattcgc ggtcggcaag ggcaaacatc
     4801 gccatcaggg ggatctggat gtaatcgagt ttcaccttgc gcgttgcatt ggcattagat
     4861 tcttcgcctt catagttctg ccccatcctt cggtatccga tgccataatt aaagccaaca
     4921 tccgaactgg caaaataata ggtgcgaaat ccgccataaa gcccgaatga cgtcacatag
     4981 gggagttctc cgtttccata tatggcctgg ttaacaatcc atcggcttgt aagggcaact
     5041 gaaggttcaa agtaaaagct tcctccgcgc tgcgacatgg ctgcaggcat cactgcaatc
     5101 atgaacaaga aaatcagaat tgtttttctt aaggatttta attcattttc gaagattgta
     5161 cgacatgtct tcattgggta acaagaatta attgatatat atccagcgat tattttcaag
     5221 atttattttg ccggccattt tcgcggtatt gccttcaccc ggaatgtaac ggataaccac
     5281 gtcggctatt ctgacatatt cttcaatatc tattttggca ctgtcaacct tatccgccca
     5341 caaaaatacg aataaacctg gtttaacctc ttttcttaaa cttctacttt cacaaactac
     5401 cggttcattt tcaggcagaa atccttcaag gtattttgcc gccatcggca tttcagtatc
     5461 cgcgacccgg atgtaatacg ctttttcagc cccggccgtc aacattttgg aagtatcctt
     5521 ccgcgatgaa ggatcttttt cctccatgat tgaaaataat tcttccgaca ggccgtcatg
     5581 aatgccatga aaaccttgtt caccgggtct gatactggtt actttcaatc cgtaaatttt
     5641 ctgaaaagcc gaaaaacgct caataacggc acaggcaaga gcagtcttac cgatgttacg
     5701 ggaattaccg gctatcataa gcatatttgg catttcgatc ataatatact attattgata
     5761 accagagtat gaaaaggtaa ttgccagtaa taaaaatttt tacagatcta cggcgttgct
     5821 gcaaaatgaa ccggagtgac tattctttta ttagttcttc caacaggctc ctgaattcct
     5881 cggccatgag gcggtccatt ttctcatgaa aacgcctgaa agccaccacc agtttatggc
     5941 cttcggccgt aagccgcgta ctgcctccct cctgccctcc gcggcttgtt tccagtaaat
     6001 caaaaccgag cagcttttca acttttttca gatcgttcca ggtgcggcga taggtgatcc
     6061 ccatctcttc acaggctttg accagcgagc cgcattcatc aattttcatg aggattttcc
     6121 atttgccatc tcccagaata ccttcccctt cgggagtaga aaaccagatt ttatactcgg
     6181 gacggacatt ttcatatttt ttcataggac agggttttaa atccgataag aagtattaaa
     6241 ttactcattt ttaggaagca aggcaatttt tatgtaaaat aattgtaccg gcaactttgt
     6301 atccggaaag accaggccca tcaattttac cggaaggata aggattgaaa tctgataaaa
     6361 tgcctgaatc agtaacggtc ataaataccg gaaccggtat ttccggtttt atccgttaag
     6421 cgacttccag agggaatcct taagggcggt aagaccctgc ctggtgtggg acgaaatgaa
     6481 tatcgccggc acccggggca gttccttcct tatcaggctc atcatttctt cgtccaggag
     6541 gtcgcacttg gtgatggcca gcacacgctc cttgtcgagt aattcaggat tatactgttt
     6601 caattcgttc agcaggattt tgtactcttt tttaatatcc ttgctgtcgg caggaatcat
     6661 gaacagcagt actgaattgc gctcgatatg ccggaggaac cgcaggccga ggcccctccc
     6721 ttcgctggcg ccttcgatga tgccgggaat gtcggccatt acaaacgata agccatcacg
     6781 atacgaaaca attcccagat ttggccttaa ggtcgtaaag ggataatcgg cgatctcggg
     6841 ttttgctgcc gacagcaccg acaacagggt ggatttaccg gcatttggaa aaccaacgag
     6901 tcccacatcg gcaagcactt tcagttcaaa aactttccat ccttcgatgc cgggttcgcc
     6961 gggttgcgca tatttaggcg cctggttcac agccgtttta aaatgatcgt tgcccaatcc
     7021 tccgcggccg cccctcatca caattttttc ctcaccatga aaggtgatct ccgtttccgg
     7081 ttccccggtt tcggcgtcct tcacgattgt tcccaacggc acttctatga taatatcccc
     7141 gccatcagct ccctttttca acgctccgga accgcctgtg ccattttcag ccattacatg
     7201 tttggtgtac ttcaggtgaa gaagcgtcca aagctgggaa ttccctttga tgataatatg
     7261 cccgccacgg ccgccgtcgc cgccatcggg gccgccgcgc ggcttcagtt tggtacgcag
     7321 gaaatgcgcc gatccggctc ccccattccc cgaacggaga aatatcttta catagtcaac
     7381 aaaatttgat gcagccattg gcttttaggg gaatttaagg tttagcgatt caggcgataa
     7441 ggataaggtg gtaaaggtta attcagggtc atcgtatttt agctgtcgga taatgactga
     7501 tgattattaa cggttttcgt ttaacagggg agtctttttc tataaactcc ggtcggcttt
     7561 ttggctgttg gcctttagct cttagctttt ggcttaaccc catatcctta agctactcta
     7621 ttattattat ttcgcgagga gttttaaacc cggagggctt taatacgaca aagcggtctc
     7681 ttctccttac cgaaatttac gttcaggctt tcgctccctg ctccttgccc ctcgctcccc
     7741 gccttttccg ttctccgttc tccgttctcc gttctccgtt ttatcttttc ttcttcatca
     7801 ccgacgcaac agccgccgac aagcgatcga taacttcttc gcggctgccg gtagcatcca
     7861 tttcgagcag tttaccctgt ttcctgtata cttccaggga aggctttgtt ttggtttcgt
     7921 actcttcgag ccggcggata atcatctccg tagactggtc ataagggcgg gccttttcac
     7981 ttcttccccg tttatctaac cggcggatga ggtcgagggt tggcacctcc atttcaacga
     8041 cacaggaaac tcgcatgttt aaacggcgca gcaagccgtc gaggatatag gcctgaagaa
     8101 tagtgcgcgg gaatcctttg aaaataaacc cgttcacatc ttcatgacgt ttcatttcct
     8161 gctcaatgag cggaataacc acttcatccg gaaccagttc cccctgggcc attttttcct
     8221 gcaccttcaa cccgacttct gtcctgttca gcacttcttt cctcagtaag gcgcctgttg
     8281 agatgtagta aaggttaaat ttccgggcaa gcagtttgcc ctgtgtgcct tttcccgatc
     8341 ctggcgaacc ggataaaacc aggttgaacc attccttttg cagcgaaagt tctatctggg
     8401 cattcagccg ctcgaaaatt tcgggaatgg gacccacgcc gtcaatcggg taataaaggt
     8461 tacgttcccg gtaatactcc ttaacgggta acgtcttgct ctcatactca accaaacggt
     8521 gccggataac atcttcggta tcatcggaac ggcctgagat ttttccccgt tcgaggaggc
     8581 gttgcaacag ctcttcgttc ggcacttcga ggctgagcat gcacgacaag gatgtattca
     8641 gtttcagcag taagccgtcg aggatgtatg cctgaacgaa tgtgcggggg aaaccgtcga
     8701 acagaattcc attgctgtcg ttgctcgaaa cgattttctt ctcgatgagt tcgaccataa
     8761 tttcatccga aaccaattct ccgcgccgga tgatatcctt caccctgttc ccgagttcgg
     8821 tttcttcggc aatttcctga cgaagcaaat cacccgtcga tatatacacc aggttatact
     8881 tctcgatgat gagttgcgat tgggtgcctt tgccggcgcc cggaggccca aataaagcaa
     8941 tattcagcat ggcagtaaga tattcccgat tttcagtttt aacaattccg gtcgaaccga
     9001 taatttatcg aagcgctgtt tttcaatcag ttatcattcc tcaatcctgt atatatcagc
     9061 cgaattcctg ccgagcccgt cataatcaag tccgtagccc accacgaaat cgttgggaat
     9121 ccggaagcag acataatcaa tagggtaatc cttcttataa gcatccggct tcatcaaaca
     9181 agctgcgact ttcacctcag cggcattcat gcttttgatt ttctcaatca ccttgatgat
     9241 tgtcaggccc gtatctacaa tatcctcaac caaaataaca tgtctgccct cgaccgggat
     9301 ctccaggccg atcaattcat ggatttttcc ggtactgtta acaccacgat aagatgaaac
     9361 cttcacaaaa gcaatttcgc agtcgatggt tatgcgcttc aacaggtcgg aggcaaagat
     9421 aaaagagccg tttaacacag ccacaaacaa gggctttttc ccttcatatt ccttgtttag
     9481 cctggcagcc agtttccgca caccggcttg aattttccgg tccggcatgt attttgtaaa
     9541 agttttgtcg tgaattttcc aagtatcggc catagtaata ttcattttcg gcaaagatag
     9601 aatatccttt acaaaggtac cagcttcctg agctttaaat atttcgcggg ttatgatggc
     9661 gaacccaggc agccgtttgt gatgcatgat gcttgatttc aacgtgcccg gggcaggtat
     9721 agcggagcgc tgggccacag gcgctggcct gtgtaaagaa tcatgatgca cggcgtcagc
     9781 cgggaacatt cacgatcaat ggtgaacagg gtgaagccgg caggctgatc aatccggaaa
     9841 tttcagtcct ggaagttaaa acttgagacg ccgggctgtt gccttttgtc tcaacaatag
     9901 tatcttcgct ttccgaaaca agaataaaaa catgaaagca atcgtcagca taattatggg
     9961 cagcaccagt gatctcccgg taatggagag cgctgccaac gtattgaatg agcttaagat
    10021 acctttcgag atgaatgcgc tgtcggctca ccgcactccg gagaaagtgg aggcatttgc
    10081 ggtgaacgct gccggccgcg gcatcagggt aatcattgcc ggcgccggga tggccgctca
    10141 tctgccgggg gtaatcgctg ctttcacacc cattcccgtg atcggcgtac ccataaaatc
    10201 cacctttgac ggtctcgacg ccctgctcgc gatcgtacaa atgcctcccg ggattcctgt
    10261 ggcaaccgtt gccgtaaacg gggcgcaaaa ttccgccatc cttgcggccc aaatcctcgc
    10321 caccggcgat aaagagttgt acgaccgggt attgaaatac aaggagaacc ttaaaaagaa
    10381 aattgcggaa gccaatgaac aactgaagga tgttcgcttc gaattcaagg ttaattaatc
    10441 ctgatagtct ccgggtttta taacttccgc atgatcatca gcccgtcacg aaccggcagc
    10501 aacaggtttt cgacgccgtt atgatgttgt atatattcat tgaaagcgac gatgctcctg
    10561 gtttcgcgat cgactgatgc attcttgtcc gtcacacggc cgtaccataa cacattatcg
    10621 gcaatgatga aacctcctcc agcaacctta ccgaacacca ggtcgaaata gcggatataa
    10681 tcgtccttgt tggcatcgat aaacaccagg tcaaaagggc cttcgatttt ttcaacaatt
    10741 tcgaatgcat cgccgatatg aagtatgatt ttctcagaaa ggccggcaag gctgaaatac
    10801 cgccgggcga tatcctccat ctccggattg cattccacgg tgtgcagcat cccgttttcc
    10861 cggattcctt cggccagaca gatagccgaa taccctgtaa aagtccctat ttccaaaacc
    10921 agctccggct tgagcatcct gctgatcatt cgtaaaaaag cgccttgcag gtgccccgac
    10981 agcatctggg gagcggtggt tttgaggtgc gtttcgcgtg acaattgttt cagaacttcc
    11041 ggctcaggcg aagtatgtgc tccggcatat tctccgagag cccggctgat aataccgccg
    11101 gacatttcat cttcgctgaa ggcctctttg ttatttcctg ccattggtaa aaataagcgt
    11161 gcttaattta tccggatcca aaatttcgtt ggccacctca aggacatcca tatcactgat
    11221 cgcatcaacc ttgccgatca tttcactgat cgtatcaacc ttattaaaaa cgaccaggct
    11281 cttgccgatt gaaagcattt cgttctgatg cgcttccacc gaaatagcga cctgtccttt
    11341 caattgttct ttgcttattt taagccggtt gccgctcagc ttgctttccc tgagtttttt
    11401 caattctccc gtaaccaggc tgattgcctt ttcaaccatt ttcgtatcgg ttcccaggta
    11461 tatggtaaaa acgccggtat cggacaacgg ctggtagttt gattcgagat tgtatgctat
    11521 gccgtgcttt tcacgcagca ccaggttcag cacagagttc atggccggcc cgccaagcat
    11581 attgctgagc agggcaagtg cgtaacgtct attatcatga tagctatatg cccggttacc
    11641 tattataata tgtgactggt gtttcgaata cttccgggac atctcctgtt tgcaatattc
    11701 gggcggcatc aaacgttcag gagagccctc ccggaccggt tgtcgcccga aatatttttc
    11761 ggtcagcgat accagcttat caaatggaat actccctacc gaactgatga ctatttgatt
    11821 ggtggagtat aaacgcctgg taaaatcaaa cgcctgctcc cgaccgaaac gtttcagatg
    11881 agccggcgta cccaggatgt tcttcccgag gggatgatcg ccaaagataa gcccgtcgaa
    11941 ttcatcctgt atccattcca tcggactgtc cttgtatgaa ttgagttcat caatcaccac
    12001 atccttttct ttcttcattt cagcttcagg aaaagtagaa ttgaaaagga tatcggcaaa
    12061 taattcaagg gtgcggctat aataggcaga aagaaaggat gcatagacgg tagtttcttc
    12121 ttttgtggtg taggcgttaa gatcggcccc tacattttca atcctgtcga gcacctggcg
    12181 ggctgttcgg cgttgcgttc ccttgaaaat aagatgctca ataaagtggg ccattccctg
    12241 ctcttcgggc ttttcgtcgc gtgcgccggc atttaccacc acaccggcat gggcaaccgg
    12301 gcttcctgtt cggcggtgta ccagccgtat gccgtttgta aactgatgta tctgatatgg
    12361 agttgacaag atgggtgggt tttcgtgatt cgtgattcga aattcgtaat taatgattcg
    12421 aaattcgtgg gaattcgtaa ttatttttta tccacgaatg gacgcgaatt aagcacgaat
    12481 g
//
LOCUS       DAOPYV010000078        12453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_5668_length_12453_cov_13.454347, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000078 DAOPYV010000000
VERSION     DAOPYV010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12453
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5668_length_12453_cov_13.454347"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1546)
                     /gene="hisS"
                     /locus_tag="PKI35_09770"
     CDS             complement(176..1546)
                     /gene="hisS"
                     /locus_tag="PKI35_09770"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNX44712.1"
                     /translation="MSTPSIPKGTRDFTPLEMARRNYIFDTIRNVFTLSGYQPIETPA
                     MENLDTLLGKYGDEGDKLLFKVLNSGDFLAGSNDKALSDKDSGKLLKQISEKGLRYDL
                     TVPFARFVVQHRNEIIFPFKRYQIQPVWRADRPQKGRYREFYQCDVDVVGSNSLIYEA
                     EMARIADEIFLRLGIGVVIEMNNRKILAGIAEIIGEADKITDITVAIDKLDKIGIDGV
                     NAELREKGLSDEKINKLQPILNLQGSNGEKLDQLESVLATSDTGIAGIAEMRAVFRYV
                     ETLRLISEIRLNLTLARGLNYYTGAIIEIKARDVQIGSISGGGRYDDLTGIFGMPGVS
                     GVGISFGADRIYDVMNALSLFPESTAHSTKVMVANFGADEEMASLAFLHRLQKARIAS
                     EMYPEPAKMKKQFAYANQKQIPYIVIIGSDELASGLYQLKNMETGEQQKYPPAEIIAK
                     LLGLNV"
     gene            complement(1695..2171)
                     /locus_tag="PKI35_09775"
     CDS             complement(1695..2171)
                     /locus_tag="PKI35_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HNX44713.1"
                     /translation="MIKITDTLLDEVIAKAKASPRKRMNYNFHPELSAPVQRLLNALE
                     PWTYIRPHKHNTKEESFVLLRGAVLAVSFNDDGTINDHAVLSATNGITGVEFEENTYH
                     MLTSLESGSVVYEIKEGPFIPHSEDNSAPWAPPEGTDQCIPFLQSVFDRLDITLGL"
     gene            complement(2198..3874)
                     /gene="ettA"
                     /locus_tag="PKI35_09780"
     CDS             complement(2198..3874)
                     /gene="ettA"
                     /locus_tag="PKI35_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNX44714.1"
                     /translation="MSDDKKIIFSMIGVGKIFPPNKQVLKDIYLSFFYGAKIGIIGLN
                     GSGKSSLLKIIAGIDKSFLGEVVFSPGYTVGLLEQEPCLDESKTVREIVQEGVQEVVD
                     LLKEYEEVNNKFMEPMSDDEMNKLIEKQGRLTELLDHHDAWELDAKLERAIDALRCPE
                     PETPISVCSGGERRRVALCRLLLQAPDILLLDEPTNHLDAESVEWLEVHLKQYKGTVI
                     AVTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKTRRLEMEEKTESKRRKTLER
                     ELEWVHMAPKARHAKGKARLNAYEKMLDEDVKQKEERLEIFIPNGPRLGSNVIEANNV
                     SKAFGDKLLFEGLNFSLPPGGIVGVIGPNGAGKTTLFRMIMGFENPDHGEFKVGETVK
                     IGYIDQAHASIDPEKSVWEVISEGNEQIQMGGRLINSRAYVSRFNFGGSDQGKKTGIL
                     SGGERNRLHLALTLKSEANVLLLDEPTNDIDVNTLRALEEGLEDYAGCAVVISHDRWF
                     LDRVATHILAFEGNSQVYYFEGGYSEYEENRRKRLGDEGPKRIRYKKLTA"
     gene            complement(3941..5125)
                     /locus_tag="PKI35_09785"
     CDS             complement(3941..5125)
                     /locus_tag="PKI35_09785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX44715.1"
                     /translation="MRIFVLLSRVPYPIEKGDKLRAFHQLRCLSKKHEIILVALNQGP
                     VDEKAILKLKEFCHQVHVIRFSYFRIAINLIRVLFTGKPFQVGYFYNRKARLKIRRIL
                     AETRPQHIFCQLLRVAEYVRHVEIPKTIDYQDVFSMGMKRRAETSAWWLKPLMLMEYK
                     RLLHYEKVIFNDFENKTIISVPDRDLIPHPDKEQIVVVPNGVDHSYFKTLQRKKTHDI
                     VFTGNMGYPPNVNAAEYLVSEIIPLVRRKVPGVSVMLAGANPHPRVQALSGDKIRVTG
                     WVEDMRESYASSRIFIAPMRIGTGLQNKLLEAMSMGLPCITSVLANQALGAAEGSEIL
                     VGRSPEEFAGHIVRLLTDEDFALTVASNGKAFVQRTYDWENSTLILEKLICAVHVPRN
                     EN"
     gene            complement(5583..6728)
                     /locus_tag="PKI35_09790"
     CDS             complement(5583..6728)
                     /locus_tag="PKI35_09790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX44716.1"
                     /translation="MNAMITGLIKSGHSVKVLAANTNKYHINPETLPGEFIRNSAIEF
                     VDIDLSINIRDALHNFIRGKSYHVARFRTPAFEKKLIEILQQDEFDIVQAEMLYMTTY
                     LDTVRKYSKAKFILRSHNIEHLIWQRIASNTHNPLKRFYLKHLFRTLRDYELDMLKKT
                     DGIVAITPTDAAFLKKHSDGTPVISIPFGIDLERYAVPMAQPGKTDLFHIGSMNWFPN
                     EEGILWFLSKVWPLINQEIPDLKLYLAGREMPEWLKNLDSKGVNIIGEVPDALEFMLQ
                     HAVMVVPLFSGSGIRIKIIEAMASGRAVISTGIGAEGIHVTHGENILIANTVEDFLEM
                     IKKVQDEEFRCEIGRNARDLIMNEHNNTLLMNRLTTFYLEIAGNPPL"
     gene            complement(6936..8384)
                     /locus_tag="PKI35_09795"
     CDS             complement(6936..8384)
                     /locus_tag="PKI35_09795"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNX44717.1"
                     /translation="MKKLYVLIFRSYAGPAIATFFISVFVLLMQFLWRYVDDLVGKGL
                     EWKIILQLLYYASYTFVPMALPLSILLASLMTFGNLGERYELVAMKAAGISLRKIMTP
                     LIILSALITIGAFVYSNTVFPYANLKFRTTLYDVRQKKMALNIREGMFYSGIEGYVIR
                     IGKKAPDGVRIYDVVIYDHTDFMGNNKFTLADSGRMEQSADGDYLLVTLFNGWNYEEK
                     QGRDSETKRPFQRTLFSEEIVKFDLTQFRLSRTNEEFFRNNFLMLPNRELSHASDSIG
                     RELESRRKDFGSALLNNYQYLRASQLLDSNCKAVLPSAKAADLLAGVQDKDRIMLINN
                     ALGIARNVKQSFEFNKQAIKEKATLKAKFDIEWHRKFTLSFACLALFLIGAPLGAIIR
                     KGGLGLPLVVSVLLFVIYHVISFTGEKAVKSGVLLAWKGMWISTLIFLPIGIWLTYKA
                     ATDSPIMESDSYKRFVSKLLSVFRKKSKKEDL"
     gene            8672..9898
                     /locus_tag="PKI35_09800"
     CDS             8672..9898
                     /locus_tag="PKI35_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44718.1"
                     /translation="MNNSANTMKYTLTEVSDKRTRRSFCDVMRILYKNDPNFICPLDD
                     TIEGIFDPQRNIFFTHGEAVRWIMKNEKGELVGRVAAFINTKKAYTFDQPTGGMGFFE
                     CINNKEAAFVLFDTCKEWLQARGMEAMDGPINFGENDNFWGLLVDGFMPQSFGMNYNP
                     PYYRQFFEDYGFQPYFEQVTNHLDYTKPFPERFWKIAERVSRNPEYNFRHFTWKESEK
                     FISDFQEIYNDAWQYHENFTPIEPDVLRGELMQVKQLMVPEFIWFAYHGNEPIAFEVM
                     FPDFNQVLRYFNGRPDLWSKLRIALKGVKNIYNRTRITVMGVKTHYQRSGIESALFWY
                     MESVMKHLHDQYKEVELSWVGDFNPRMQTIHEATGATFGKRHITFRKIFNGNPGFTKP
                     ARLHTADLSKPVEEKK"
     gene            9973..11802
                     /locus_tag="PKI35_09805"
     CDS             9973..11802
                     /locus_tag="PKI35_09805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44719.1"
                     /translation="MKKLLPLVFSIVVVGAYAQQRATLPSNLLNYQVKAEKYAIKETA
                     NMMTDVNHYVASYDALSEAEMGYTVYDLQSNASSPSNRFYIYPSGEMAGVWTFGTGPT
                     SYSNRGTGYNYFDGTSWGPNPTNRIETSKSGWTNYAQLGATGEIVCTHHNSAGLIINK
                     RSTRGTGAWTESILPGPAGAVDISWPRMVTSGTNKTNVHIIASTYSAYAGQDLALLYY
                     RSTDGGETWDKTHVIPEQMNSTHYAGFSGDIYSWAEPLGDNIAFIVNDPDEDLFVMKS
                     ADNGETWEKIVIWQHPDPLGTGTPVLDSLWGPDGAAHLTFDKFGKLHVVFGVYKSNPV
                     GGTWFPYSGGVAYWNEDMPTWTGGTPEWQINCLNPDSLDLTGNLIGYPLDLNGDGIFN
                     VLGEAGLYYIAVVSMPQITIDENGDGMVVFAGITENYDNDAQDYRHLWARSFSNFGAC
                     FGKFVDLTGDDIHLFDEAVFPTFSPTSNDFFGLIAQIDTEPGLAVRGDEDQPGDNYMV
                     CFTMPKDEFTGTKDVKETILSSVYPNPASDNAMITFRTEKASSVTIRISSLTGQAVYE
                     RTLDVLTSGDHRVSVPVQDYKAGLYLYTVTAGNVTNSGKLVVR"
     gene            11954..>12453
                     /locus_tag="PKI35_09810"
     CDS             11954..>12453
                     /locus_tag="PKI35_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44720.1"
                     /translation="MKRCLLFLLAMGIGVLTFAQYQKAALPNSVRNIAVPKKCAVTET
                     TNPFTTVANSYVESPVSYIEDEVGYTVYDLQSNGSTPYGRLTQFDDGTIAAVYTMAIN
                     PTAYNDRGTGYNYFDGTAWGPSHVTRLESVKSGWPSHAQLGANGEVIVSHRSGTAGLK
                     LLKRDV"
BASE COUNT         3123 a         2885 c         3020 g         3425 t
ORIGIN      
        1 ttgtgagttt tgggttttga gttctgagtt ttgcattctc cgttctccgt tctccattct
       61 ccgttctccg ttttgccctt tgctccttgt cctttgccct ttgacttttg ccctttgaca
      121 tttaactttt gacttccttt caccaagatc tctctccttc gcaatcttct ccttctcaaa
      181 cattcagtcc caggagcttg gctataattt cggccggagg atatttttgc tgttcaccgg
      241 tttccatgtt tttaagctgg tataatcccg aagccagctc atcgctgccg atgattacga
      301 tataaggtat ttgtttctga ttggcataag cgaattgttt tttcatttta gccggttcgg
      361 gatacatttc cgatgcgata cgcgcttttt gcagcctgtg cagaaaagca aggcttgcca
      421 tctcttcatc agcgccaaag ttggcaacca ttacttttgt ggaatgtgct gttgactcgg
      481 ggaaaagaga taatgcattc ataacatcat agatccggtc ggcgccgaat gagataccta
      541 ctcccgacac tccgggcata ccgaaaatgc cggtgagatc gtcatagcgt ccgccgccgc
      601 tgatgctgcc tatctgcaca tcccgggcct tgatctcgat gatggcgccg gtataatagt
      661 tgagcccgcg ggccagggtc aggttaagcc tgatttccga aatgagcctt aacgtttcaa
      721 catagcggaa cacagcccgc atttctgcaa taccggcaat ccctgtgtcg gaggttgcca
      781 ggacagattc aagctgatca agcttttcgc cgttgctgcc ctgaaggtta agaatgggct
      841 gcagcttatt tattttttca tccgaaagcc ctttttcacg caattcggca ttcacaccat
      901 cgattccgat tttatccagt ttatcaattg caacggttat gtcggtaatt ttatcggctt
      961 cccctataat ttcggcaatt cccgccagaa ttttccggtt gttcatttca atgactaccc
     1021 cgataccgag cctcagaaaa atttcatcgg ctatgcgggc catttcggct tcatagatca
     1081 gggaattgct gccgacaaca tcgacatcgc actggtaaaa ttcgcggtag cggcctttct
     1141 gaggcctgtc ggcgcgccaa acaggctgga tctggtaacg cttgaacggg aaaatgatct
     1201 cgtttcggtg ttgaaccacg aaccgggcaa agggtacggt aagatcgtaa cgaagacctt
     1261 tttcgctgat ctgcttcagc agttttcccg aatctttgtc cgaaagtgcc ttgtcgttgc
     1321 ttcctgccag aaagtcgccc gaattaagaa ccttaaaaag gagtttatcc ccctcatcgc
     1381 catactttcc taacaacgta tccaggtttt ccatggctgg cgtctcgatg ggctgatatc
     1441 cggaaagcgt gaaaacattc cggatggtat cgaagatata gttccggcgt gccatttcga
     1501 gcggtgtgaa atcgcgggtg cctttgggta tggagggtgt ggacatcggg gcaggaataa
     1561 ggtttaacgt ttacagggtg aagggtggcg tgagtgaatg cgagtttaac taaagcaatg
     1621 gattgatgtt actaaaggta taaaacttct gacgttttga taattaatct gttgactgtt
     1681 agttcagaat gttctcaaag tccgagcgta atatccagcc ggtcgaaaac cgattgcaga
     1741 aacgggatac attgatcggt gccttccggg ggagcccatg gggctgagtt atcttccgaa
     1801 tgaggaataa atgggccttc cttgatttca taaaccactg atccgctttc gagcgaggtg
     1861 agcatatgat aggtgttttc ttcgaattcc acccctgtga taccgtttgt agcgctcaaa
     1921 acggcgtggt catttatggt tccgtcatcg ttaaaggata ccgccaacac ggctccgcgc
     1981 agcaacacaa aggattcctc ttttgtgttg tgtttatgcg gccggatgta agtccacggc
     2041 tcgagggcat tcagcagcct ttgcacaggg gcgcttaatt cgggatgaaa attatagttc
     2101 atccttttcc gcggcgaggc tttggctttg gctatcactt catccagcaa ggtatcggtg
     2161 attttgatca tagtgatttt tatcctgttt aatgatttca ggccgttagt tttttatagc
     2221 ggatgcgttt cggcccttcg tcgccgagtc gcttgcggcg gttttcttca tattcggagt
     2281 aacctccttc gaaataataa acctgtgagt tgccttcaaa ggccaggatg tgggtggcta
     2341 cgcggtcgag aaaccaacgg tcgtgtgaaa ttactacggc gcaaccggca taatcttcca
     2401 gcccttcttc cagagcgcgc agggtattaa cgtcgatgtc gttggtaggc tcgtcgagca
     2461 acagtacgtt ggcttccgat ttcagggtta gcgccaggtg taagcggttg cgttccccgc
     2521 ccgacaagat gccggttttc ttaccctggt cggaaccgcc gaaattaaac cgcgaaacat
     2581 aagcccggct gttaatcagc cttccgccca tttggatctg ttcgttccct tcgctgatca
     2641 cttcccagac gctcttttcg ggatcgatgg acgcatgggc ctggtcgata tacccgattt
     2701 ttacggtttc gcccacttta aactcaccat gatcggggtt ttcaaagccc atgatcatcc
     2761 ggaaaagggt ggttttccca gcgccgttag ggccgattac ccccacaatg cctcccggag
     2821 gcagtgaaaa gttcagcccc tcgaaaagca gcttatcgcc gaaggctttc gaaacattgt
     2881 tggcttcaat gacgttactg ccaagccggg gcccgttggg gataaatatt tcgagacgtt
     2941 cttccttctg tttgacgtct tcatcgagca tcttttcata ggcattcaga cgggctttcc
     3001 ctttggcatg acgggctttc ggggccatat gtacccattc aagttcacgc tcaagggttt
     3061 tccggcgttt gctctcggtt ttttcttcca tttcgaggcg cctggttttt tgttcaagcc
     3121 atgacgaata gttgcctttc catggaatgc cttctccccg gtcaagctca aggatccagc
     3181 cggccacgtt atccaggaag taacggtcat gggtaacagc gatgacagtt cccttatact
     3241 gtttcaggtg aacttccagc cattccacgc tttcagcgtc gagatgattg gtgggctcat
     3301 ccaggagtaa tatgtcggga gcctgcaaca gaagcctgca taatgctacg cgtcgtcttt
     3361 ctcctcctga acaaacactg atcggcgttt caggttccgg gcatcgaagg gcgtctatag
     3421 cccgttcgag tttagcgtcg agttcccagg catcgtggtg gtcgagcaat tcggtaaggc
     3481 gtccctgttt ttcgatgagt ttgttcatct catcgtcgga catcggctcc atgaatttat
     3541 tattcacttc ctcgtattct ttgagcaggt caacaacttc ctgaacgcct tcctgaacta
     3601 tttccctgac ggtttttgat tcatcgaggc aaggctcctg ctcaagcagg ccaacggtat
     3661 agcccggtga gaaaacgact tcgccgagaa aggatttatc gattccggca atgatcttca
     3721 gcaaactcga tttaccggag ccgttcaggc cgatgatgcc gatcttggcc ccgtaaaaaa
     3781 atgacaggta tatatccttc aggacctgtt tgttgggcgg aaaaattttc ccgaccccga
     3841 tcattgaaaa gattatcttt ttatcgtcac tcatggacat tagaagttgt tgttaaagag
     3901 gacagtggca gccggccgga tcagaatcca tcagctttag tcagttttca ttccggggaa
     3961 catgtactgc gcatatcaat ttttcgagaa tcagcgttga attctcccag tcataagtcc
     4021 gctggacgaa agcttttccg ttagaggcaa ctgtaagggc gaaatcctcg tcggtgagga
     4081 gcctgacgat gtggccggca aattcttcgg ggcttcgtcc cactaatatt tccgaacctt
     4141 cggctgcccc cagggcctgg ttggccagca cagaagttat gcagggaagt cccatcgaca
     4201 tggcttcgag aagtttattc tggaggccgg tgccgattct catgggggcg atgaaaatac
     4261 gggatgaggc ataactttcg cgcatgtctt caacccaacc ggtcaccctg attttgtcac
     4321 ccgataatgc ctgaaccctg ggatggggat tggcgccggc cagcattacg ctgacgccgg
     4381 gaaccttccg gcggaccaac gggatgatct cgcttaccag gtattcggct gcattcacgt
     4441 tgggcggata acccatattg cctgtaaaaa ctatatcatg agtttttttc ctttgcaggg
     4501 ttttgaaata agaatggtca acgccattgg gcacaacaac gatctgttct ttgtccggat
     4561 ggggtatcag gtcgcggtca ggaacagaaa ttatcgtttt gttttcgaaa tcgttgaaaa
     4621 tgactttctc ataatgtaac aaccttttgt actccatcag cattaaaggc ttgagccacc
     4681 atgccgacgt ttccgcccgc cgcttcattc ccatcgaaaa aacatcctgg taatcaatag
     4741 tttttggaat ttcaacatgc ctgacatact ctgctaccct cagcaactgg cagaaaatgt
     4801 gttgcggacg ggtttcggcc agaatcctcc tgatttttag tctggctttc cggttgtaaa
     4861 aatatcccac ctgaaaaggc tttccggtaa ataagaccct gataaggtta atggctatcc
     4921 ggaaataact aaaacggata acatgcacct ggtggcaaaa ttctttcagt ttcaggattg
     4981 ctttttcatc gacgggcccc tggtttaacg caacgagaat aatctcatgt ttttttgaaa
     5041 ggcatcgcaa ctggtgaaac gcgcgtaatt tatccccttt ttcaatggga tagggtacac
     5101 gcgatagcaa gacgaagatc ctcattttta atgtgttcga tgatgacaag tggcaagcct
     5161 tcagagtaat tgcaaaggta taaaacggtt ttgatatgag aaagaaactc atccttttaa
     5221 ggaagggatg gccccaatac cggaactgcg gatggaagaa ggctccctga atcaaacatg
     5281 aaccgggacg atgatatatt aatataggta tatagatagt gtggaaaagc gttgcgattg
     5341 gcagggtttt aaggtttcgg ggctggaatt tttcaggggg aagcctgagc ccgacggggc
     5401 ggtttgtacg atgaacggca aatcccggtt tctcattgct tctggtgata tttcccggtt
     5461 tggtaatgct cgcacagaaa aattcaattt tttccctgca gtttaaaacg aaccggtcaa
     5521 agccttttct tccggtcagg tttcatacag cctgattccc ggaaatgaaa accaacctgg
     5581 aattataatg gcgggttccc ggctatttca aggtagaacg ttgtcagccg gttcatcagg
     5641 agggtgttgt tatgttcgtt catgatgagg tcgcgggcgt tacggcctat ttcgcagcga
     5701 aattcctcat cctggacttt ctttatcatt tcaagaaaat cttcgacggt attggcaatg
     5761 agaatatttt ctccatgcgt tacatgaatg ccttcggccc ctattccggt agaaatcacg
     5821 gccctgccgg atgccatggc ttcgataatt ttgatccgga tgccgctgcc cgaaaacagc
     5881 ggaacaacca ttaccgcatg ttgcagcatg aattcgagcg cgtcgggaac ttcgccgatt
     5941 atgtttacgc ctttggaatc aagatttttg agccattcgg gcatttcacg gccggcgaga
     6001 tacagtttca ggtccggtat ttcctgattt atcagaggcc aaacctttga taaaaaccag
     6061 aggatgcctt cttcattggg gaaccagttc atggaaccga tatggaaaag gtccgttttc
     6121 ccgggttggg ccatcgggac ggcatatctt tcaaggtcaa ttccgaaggg tatggaaatt
     6181 acgggcgtgc cgtctgaatg tttcttaaga aatgccgcat cggtaggtgt tatggcaaca
     6241 ataccgtcgg tctttttcag catgtccagt tcataatccc tgagcgtgcg aaacaggtgt
     6301 ttcaggtaaa accgcttgag cggattatgg gtgttggatg caatacgctg ccagatcaga
     6361 tgctcaatgt tgtgcgagcg gaggatgaac tttgccttgc tgtattttct gacggtatcc
     6421 agataggttg tcatatacag catttcggcc tgaacaatgt caaactcatc ctgctgcagg
     6481 atttcaatca gttttttttc gaaagccggg gtgcggaacc gtgcaacgtg ataggatttc
     6541 cccctgatga agttgtgcag cgcgtcgcgg atatttatgc tgaggtcgat atcaacaaat
     6601 tcaatagcgg aattccggat aaattctccg ggaagcgttt ccggattaat gtggtattta
     6661 ttggtattgg ctgccagaac tttcacgctg tggcctgatt ttatcagtcc tgtgatcatt
     6721 gcattcatag cgatcggacc tccttcggca ggtggccacg gcgatttatt gcaaagcagc
     6781 aggattctca taaggcgtca ggatgtgtaa ctgaggttta ggtttaatgc acgggaggcg
     6841 tgtgtttgcg ggtggcaggg tggggcgcgg tgcaatgatg aaagaagttt tttacaggca
     6901 ttttaaatat agggattttt ctgccgggat atgcattaca ggtcttcttt tttacttttt
     6961 tttctgaaca cagagagaag ctttgagacg aaacgcttat agctgtccga ctccatgatg
     7021 ggtgaatcag tggcagcctt ataggtaagc catatcccga taggcaaaaa gatcagcgtt
     7081 gaaatccaca tgcctttcca cgcaagtaaa acgccgcttt ttactgcttt ctccccggta
     7141 aaagaaatga catggtatat cacgaacagc aataccgata cgaccagcgg caaaccaagg
     7201 ccacctttcc ggatgatagc tccgagagga gcgccgatca gaaacagcgc caggcatgcg
     7261 aaggagaggg tgaacttgcg gtgccattca atatcgaact tcgccttcag cgtggctttc
     7321 tccttgatgg cctgcttgtt gaattcgaag gattgcttga cgttgcgggc tatgcccagg
     7381 gcattgttga tcagcatgat cctgtcttta tcctggactc ctgccagaag atcggcggct
     7441 tttgccgacg gaagaaccgc cttgcagttt gagtcgagga gctgggaagc gcgcagatac
     7501 tgataattat taagcaaggc tgacccaaaa tccttcctgc gcgactcgag ttccctgcct
     7561 attgaatcgc tggcatgtga caactcgcgg ttgggaagca tcaggaaatt attgcggaaa
     7621 aattcctcgt ttgtccgtga aagccggaat tgggtaagat cgaatttcac aatttcttcg
     7681 gagaacaggg tgcgttgaaa aggccttttt gtttcgctgt cgcggccctg tttttcttca
     7741 tagttccagc cattgaacag ggtaaccagg aggtaatcgc catcggcgct ttgctccatt
     7801 ctcccggagt cggccagcgt gaatttgttg tttcccatga aatcggtgtg gtcataaatg
     7861 acaacatcgt aaattcttac tccgtcgggc gccttttttc cgatcctgat cacataccct
     7921 tcgattcccg aatagaacat tccttcgcgt atattcagcg ccattttctt ttgcctcaca
     7981 tcgtagagcg ttgtcctgaa tttcaggttt gcataaggaa agacggtgtt ggaataaaca
     8041 aatgctccga tggtgatcag cgccgaaaga atgatcaaag gcgtcattat tttccggagc
     8101 gaaatgcctg ccgctttcat agcgacgagt tcataccttt cacccaggtt gccgaaggtc
     8161 atcagggagg caagcagtat actcagcggc aacgccatcg gaacgaaagt atatgaggcg
     8221 taatacagga gttgcaggat aattttccat tcaagccctt ttcctacgag atcatcaaca
     8281 tatcgccaca ggaactgcat cagcaggaca aaaaccgaga taaagaaagt tgcaatggcc
     8341 gggcctgcat aagatcggaa gatgagcaca tacaactttt tcacgccgct ggagtattat
     8401 ttgccctgca aaggtaatga gttaaagaat aaaaggtgac cttccggatg cggtaagttt
     8461 tggatgaaca gaaatttcag gagaaaatca ggatgcctgt tttcttatat cagccgggcg
     8521 ccaataatga tatgagtgct atcgaatggt ggcgttcaga tggttgctgc atggcatggc
     8581 agttcttata ttataatagc aggttaccgg gaaatatcgc ttttatggaa aagcatttaa
     8641 tcaggaattg attttatcta catttgccgg aatgaataac tctgccaaca ctatgaaata
     8701 tacgttaacg gaagtaagcg ataaaaggac aaggcgcagt ttttgcgatg tcatgaggat
     8761 tctgtacaag aatgacccaa actttatttg tcctcttgat gacacgattg aagggatctt
     8821 tgatcctcag cggaacatat tttttacgca cggagaagct gtccgatgga tcatgaaaaa
     8881 tgaaaagggg gagttggtcg ggcgcgtggc agccttcatc aataccaaaa aggcttacac
     8941 ttttgatcag cctaccgggg gaatgggttt tttcgagtgc attaataata aggaggcagc
     9001 cttcgttctt tttgatacct gtaaggaatg gctgcaagcc cgggggatgg aagccatgga
     9061 tggtcccatt aactttggcg agaacgataa tttctggggg ctgctggtcg acggatttat
     9121 gccccagagt ttcggaatga attataatcc gccttactac aggcagttct tcgaagacta
     9181 cggatttcag ccctattttg aacaggttac aaatcacctt gattacacga aaccattccc
     9241 ggaaaggttt tggaaaatag ctgaacgggt gagccggaat cctgaatata actttcgcca
     9301 tttcacctgg aaagaatctg aaaaattcat tagcgatttt caggagatat acaatgatgc
     9361 ctggcaatat catgaaaatt ttacccctat tgaaccggac gtgctgaggg gcgaactgat
     9421 gcaggtgaaa cagctcatgg tccctgagtt tatctggttt gcctatcacg gcaacgaacc
     9481 gattgctttc gaggtcatgt ttcctgattt caaccaggtg ctcaggtatt ttaacggacg
     9541 tcctgacctc tggtcaaaat tgcgtattgc actgaagggc gtaaaaaata tttacaaccg
     9601 tacccgtatt acggtgatgg gcgtaaaaac gcattatcag cggtcaggca tcgaatcggc
     9661 attattctgg tatatggaaa gcgttatgaa acatttgcac gaccaatata aggaagtaga
     9721 attatcatgg gttggcgatt ttaacccgcg gatgcaaaca atccatgaag ctaccggcgc
     9781 cactttcggg aaacggcata ttactttcag gaaaatattc aacggaaatc cgggcttcac
     9841 caagccagcg cgattgcata cggcagacct ctcaaagccg gttgaagaaa aaaaataact
     9901 tgcttcacgc aagtaatgcg aaatatacta aatttacaaa tggatttctt aaacattaaa
     9961 cccctaataa acatgaaaaa acttttaccc ttagtatttt cgattgtagt ggttggcgcc
    10021 tatgctcagc aaagggccac gctgccatcc aacttgctta attatcaagt gaaagctgaa
    10081 aagtatgcaa tcaaagaaac tgccaacatg atgacggatg tcaatcatta tgtggccagc
    10141 tatgatgcat tatcggaagc tgaaatgggt tacacggttt atgacctgca atccaacgca
    10201 tcctctccca gcaaccgctt ttatatttac ccaagcggtg aaatggccgg tgtatggaca
    10261 ttcggcacag gccctacttc atattcgaac aggggtacgg gttataatta ttttgacggc
    10321 acatcgtggg gtcccaatcc taccaaccgc attgaaacat caaagtcagg gtggaccaac
    10381 tatgcccaac tcggcgctac cggcgaaata gtatgtacgc atcataattc tgccgggctc
    10441 atcattaaca aacgcagtac gcgcggcacc ggagcctgga ctgaaagcat cctgccggga
    10501 cctgccggtg ctgtggatat ttcatggccc cgcatggtga catcaggaac caataaaacc
    10561 aacgtccata tcattgcaag cacttactct gcctatgcag gtcaggacct tgccctgctt
    10621 tattatcgtt cgaccgatgg cggcgaaacc tgggacaaga cccacgtaat tcccgagcaa
    10681 atgaattcaa cccactatgc cggttttagc ggtgatatct atagctgggc cgaaccactc
    10741 ggcgataaca ttgctttcat cgtgaacgat cccgatgaag accttttcgt aatgaagtca
    10801 gccgataacg gtgaaacctg ggaaaagata gtaatatggc aacatccgga tccattggga
    10861 accggaactc cggtgcttga ctctttatgg ggtcccgacg gagccgctca cctcacgttc
    10921 gacaaattcg ggaaactgca tgttgttttc ggcgtttata aatcaaatcc cgtaggggga
    10981 acctggttcc cttacagtgg cggcgttgct tactggaacg aagatatgcc gacctggacg
    11041 ggcggaaccc ccgaatggca aatcaactgc ctgaatccgg attcgctgga tctcaccggc
    11101 aatttgatcg gttatcctct tgaccttaac ggagacggta tctttaatgt tttaggcgaa
    11161 gcgggcttgt attatattgc ggtggtttca atgccgcaga tcaccattga tgaaaacggt
    11221 gacgggatgg tagtttttgc tggaattacc gagaactacg ataacgatgc acaggattac
    11281 agacacctgt gggccaggtc attctccaat ttcggggcat gctttggcaa atttgtcgac
    11341 cttaccggag acgatattca tttatttgat gaagctgtct ttccgacatt ctccccgact
    11401 tcaaatgatt tcttcgggct tattgcccag attgacaccg aaccaggcct ggcagtgcgc
    11461 ggtgatgaag accagcccgg tgataattac atggtatgtt ttacgatgcc gaaagatgaa
    11521 tttacaggca caaaagatgt gaaggaaacc atattgtctt ctgtttatcc caatccggct
    11581 tccgataacg ccatgatcac cttcaggact gaaaaggcct catccgtgac tatcaggatc
    11641 agttctttga caggacaggc ggtttatgaa cgcaccctgg atgtacttac ttccggcgac
    11701 catcgtgttt cagttcctgt tcaggattac aaagcaggcc tgtaccttta cactgtaaca
    11761 gccggtaatg ttacaaattc aggcaaactg gttgtccgtt aaacggatca cacctgataa
    11821 caaaagaaac ccggtcccag gccgggtttt ttttataaaa aaatttttga ttaatgaaaa
    11881 agtaattcaa tttttttaat ctttgcaata actaaatctt aaatcattca taaccaaacc
    11941 aaattgctct actatgaaaa gatgtttact atttttattg gctatgggaa taggcgtttt
    12001 gacttttgcc cagtaccaga aagctgccct gccaaactcg gtccgcaata ttgcagttcc
    12061 gaaaaaatgt gcggtaactg aaactaccaa tccatttacg acggtggcaa actcctatgt
    12121 tgaatcaccg gtatcttaca tcgaagatga agtaggttat accgtttatg acctgcagtc
    12181 gaatggttcg accccttatg gccgtttaac ccagtttgac gacggtacga tagctgcggt
    12241 ttataccatg gctattaatc ctacggccta taatgaccgt ggaaccggat acaattattt
    12301 tgacggaacc gcctgggggc ctagccacgt aacccgcctg gaatcggtaa aatccggatg
    12361 gccttcacat gcccagttag gcgcaaatgg tgaagtgatt gtttcgcacc gcagcggaac
    12421 ggcaggcctc aagttactga aacgcgacgt taa
//
LOCUS       DAOPYV010000079        12415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_5696_length_12415_cov_12.430583, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000079 DAOPYV010000000
VERSION     DAOPYV010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12415
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5696_length_12415_cov_12.430583"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="PKI35_09815"
     CDS             <1..1220
                     /locus_tag="PKI35_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HNX44721.1"
                     /translation="DASSFPKTLRLTIMSDISVYFRPVNTFELPAAPNPLYKRMGEVI
                     DIHTAEGQFPDLENIDIALIGVDEDRQAVSNEGCSVAPDFVREYFYRLYPGDYPLRIA
                     DLGTIIRGHQVSDTYFALSTVIGHLIERKIVPVVIGGSQDLTYAVYKAFESLKRIINI
                     AAVDNMFDLGEGEAEVNSRNYLSHIILHKPNYLFNYTNIGYQTYFVDQGAITLMKNLF
                     FDINRLGMARANLPEIEPLVRNSDVLTFDISAVRQTDAPGNGNATPNGFYGEEACQIM
                     RYAGLSEKLSAMGIFEVNPAFDRHGQTEHLVAQMIWYFIDGFYHRQDDFPSAESDDFI
                     RYTVQLTNHEEGIIFYKSKKTERWWMEVRCTEDIRHKYLRHYIVPCTYGDYQTACSDD
                     VPDRWWQAYQKLM"
     gene            complement(1278..2081)
                     /locus_tag="PKI35_09820"
     CDS             complement(1278..2081)
                     /locus_tag="PKI35_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HNX44722.1"
                     /translation="MIYNTDIRKNHTSRFHFTGMITALLLIMLTIDGIAQKPEGYIRY
                     LRTGNWAKQMAAVDYLSKQQRDRFMYMWSSRAEYKSFTNLWFSPAGSKYEDSEEIADR
                     DREGGYDWRKEVFLIMRNFETSRIHDAIQLNGKVYVIEDTLNYPQWKILNDMKEVAGH
                     ICMNAFYEDTLKMQKITAWYALDLPVSAGPERLGGLPGVILEVDINDGAMLITADKVE
                     MKPLTDELNPPKKMKGKKVSEEQYLTVLKKHFDDRRADEEPPFWGDVRY"
     gene            complement(2115..4931)
                     /locus_tag="PKI35_09825"
     CDS             complement(2115..4931)
                     /locus_tag="PKI35_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX44723.1"
                     /translation="MRKFHVALLLFVLGFPAFLTAQHPSKVTIKGIASDSTNAEAGYA
                     TVMLLNRKDSTLVNFTTTNEKGIFSFNGVRNIPYLLKISHVSYIPYQKALEPSSTEIN
                     DLGILRLKPISQTLMEVVIKAAKAPLRIRGDTIEYDATTFKIPPGSTVEDLLRRLPGI
                     DVDADGNIQTQGKDVKRVYVDGKTFFGDDPKSVTKNLDAEAISKVQVFDEKSEQTKLT
                     GVDDGTKEKAMNLGLKEEYKKGSFGKATLGGGTSERWAARGSYNRFDEKNQLSFIGYG
                     NNINQSGVNWEDYGEFKGQNTFNDYDNGDFGFEGGGSGTIIITDNSFMGSDRRGYSRN
                     YGGGTNYNFDNKKTKFNASYFYNQTRQDLDQYTSKQTFLEGGTVLNTDTVSQNNFLDN
                     HSIGSRFEHDIDSNNKVIVKANIKFSNNNTSNERWQHFATGVDTPLNNLSTDNETQLD
                     SWTLTTAAIYRHRFLKKGRSLALSAGYNNTHSANLDSLYSLNEFFTATTFTEQIRQLN
                     DKSSDVSQVKASALYTEPILKSWFWETFYNFNQSESVTDRQVNNPEGNTRIDSVSVNA
                     KNNTLYNRIGSGIRYSHNGLNISAGVALQQLKLDGEYLQYRAKPLEDTTLNFTQSYTN
                     WVPNLSMNYQLPNNMYMSLGYSYNINEPSASDLQAVPNSNNRNFLTEGNPDLLPERSH
                     SLNFSYNYWNPSSFASVGIYSSYSIYEDQIIYNQWIKTVDSVGLQTITRPSNISGGNG
                     LNVNFWSSFPIIKTKFSTSLYGGYSAGKSASYVNDIRNDNNDNGFNIGLGFNITPSPK
                     LILDLRGNLGYNKIEYSIQKEQNQNIQNHSVYSSLKWQFMKKTFLESSFSYSMYVNDR
                     FDFDRRIPILNASLRRILGTKNRFEMRLAAFDIFNKRVNISQYGSSNYVTNSMTPTLA
                     RYFLLSLSYNLRGYDDKLSKNNMF"
     gene            complement(5119..5670)
                     /gene="ruvC"
                     /locus_tag="PKI35_09830"
     CDS             complement(5119..5670)
                     /gene="ruvC"
                     /locus_tag="PKI35_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNX44724.1"
                     /translation="MESDKIILGIDPGTNIMGFGIIEVKGKQLNLIALDVIKLDHLDT
                     QPLKLKNIFVKVLALVDHYHPDEVALEAPFFGKNVQSMLKLGRAQGVAMAAALYRDIP
                     IFEYSPRKVKQSITGHGNASKEQVSAMLNRMFVIAEQPKYLDATDAVAVATCHYFQRN
                     TSETGKNYSGWSSFISNNPDRVK"
     gene            5705..6727
                     /locus_tag="PKI35_09835"
     CDS             5705..6727
                     /locus_tag="PKI35_09835"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNX44725.1"
                     /translation="MNRSKVNKTLNIFLRLLILAVSFGYIGLKIFGERGTINTDVILQ
                     NLVSQPYVVPFSILLFLLMLANWSLETLKWRFLVSKVENVGFGSACVAVLTGITVSIF
                     TPNRVGEFFGRAFVLRKANPAKAILLTLVGSMSQLLVTVMAGSFALAFFLPKYVPFSG
                     MWSSGIYLSVVAGMAVVNLVFVLFYFNVPLLGSFSAWLAAKNFSRLSGYLRVLNDFSR
                     KELLKALLFSLTRYVVFSVQFFLVLLLFGVPVTPLSAVLAIPVIYLALAAIPTVALSE
                     LGVRGSVALFVLGMVIPGGALNHTIQAGLSAAIVSAATALWFINLAVPALAGMLFIYR
                     LKFVRQ"
     gene            6724..7863
                     /locus_tag="PKI35_09840"
     CDS             6724..7863
                     /locus_tag="PKI35_09840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX44726.1"
                     /translation="MTWIIVTLALLALYAVLMLMITAGWFSMPVFHGSGAEPVKISVI
                     IPVRNEEKHLAALIGDMMAQDHPATLFELIIVDDHSGDHTSEVVHSFMENFPLERISY
                     MNLGDFPGREGKKDAIETGVENASGTLIITTDADCRLPKTWLSTVASYYSVYKPKLLL
                     APVRMCPGNTLFGRLQSLEFTSLIAASAGSASAGFPLLANGANLAFEKEAFFQVGGYS
                     SNRHYPSGDDIFLLQSMRKQFGPKAVHYIKSKLATVSTPAQAGLKEFLNQRLRWVSKN
                     GGYRDMNVLTSGSLVYAVNALLPALMVAGFFNGFAWKAACIWFMVKLAVDFPILAGVT
                     SFSGLGKTILLLPLMEILNSLYTTFIGFAGLVIPYEWKGRKFNSL"
     gene            complement(8860..11547)
                     /locus_tag="PKI35_09845"
     CDS             complement(8860..11547)
                     /locus_tag="PKI35_09845"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HNX44727.1"
                     /translation="MIRPTIFLACFCCFGTLIVSGQEKEKPVYRSEAYSVYHDRVVQG
                     GFTAVARSAEEITSDYKSPRDGCYSPDITFKFSINLRDNEMPSGRDHKVTLQPVNGHC
                     TTTVRFGKQYVHTVKVAEGKNLKPNTLWTIRLDMREVFDAFKKQGFYTFFNGEKLFRD
                     DFKGVYIAGNAAPLSWDFNNLHTQPGLQLTDPDHDGIFETTLVMNSQDNSKRTAASWK
                     LSKDITSFPVYHSDYLISDALYNLAIEEMIKAVEPDSTFRTGKEWAGVWTRDISYSII
                     LSMAYLQPQVAMYSLMRKVKNGRIIQDTGTGGAYPVSSDRMIWAAAAWEVYKATGDED
                     WLKQAYGIIRNSVEDDMLNIYDPETGLVRGESSFLDWREQTYPRWMQPADIFNSENLG
                     TNAVHYQANRILAEMAQRLSDKKSTERYNHNAEKIRDGINRCLWMPEKGYYGQYLYGR
                     NFSSLSPRAEALGEALCVLYGIADEQTGKSVIRNVPVTDYGISCIYPQIPGIPPYHNN
                     AVWPFVQSYWALASAKAGNEKSVLESIAAVYRPAALFLTNKENFVASTGDYAGTQINS
                     GNMLWSLSGSIALVHKVLFGIEFQPDSLVFHPFVPQALNGKRVLSGFRYRGMILDIEM
                     EGYGNRIRSFSVDGIQSNRYIIPDSLMGRHQVEIILDGFSDDAPINKCPVAFSPEAPV
                     LKYEHNELRWNAAENAVEYLVLKNGKAAAHTSAGAFPVEKKEYSEYQVIAVDKNKVQS
                     FASEPVVVADTGMCNVYNPENFAGPVATRYKNYTGDGYVIISRTENLTLPVTVEIQEK
                     GSYAVSFRYANGNGPVNTENKCAVRTLQLDGEFKGTFVFPQRGKEEWSNWGYSNAVVV
                     LMDKGKHTLQLVFEPANENMNGEINEAAVDSLILWKTGE"
     gene            complement(11547..>12415)
                     /locus_tag="PKI35_09850"
     CDS             complement(11547..>12415)
                     /locus_tag="PKI35_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNX44728.1"
                     /translation="ISRAHHEGNNAVEPWLFGPEAEKACKDAISLKYSLFPYLYGCAR
                     KAHDEGLPLMRAMVMACPDDKETFNLDSQFMFGSDLLVAPVVEPNATSVKVYLPEGEW
                     ACFNDPSSRFTGNQWIDYPVALGTIPVFVRSGSVIPMMPVLQYIHEKPDAPVWFTVFP
                     KNAGKSSAELYDDDGESNDYKKDVYSLTRVKCSFKNGDITLSLEKKDFNGGYHGDKRN
                     FGIRIYTDKEPASVAMDGNKLKQVADARLRERWYAVAEKPEWSYDKKSKTLWIKFPEP
                     KDMTEITVKNSR"
BASE COUNT         2921 a         2943 c         3066 g         3485 t
ORIGIN      
        1 aagacgcatc gtcgtttccg aaaacattaa ggcttaccat catgagtgat atttctgttt
       61 atttcaggcc ggtcaacacc ttcgagttgc cggcagcgcc gaatccttta tataaacgaa
      121 tgggcgaagt gattgatata catacggcag agggacaatt ccccgacctg gaaaatatcg
      181 atattgccct gatcggcgtt gacgaggacc ggcaagccgt ttcgaacgaa ggctgttccg
      241 tggcccccga ttttgtgcgt gaatattttt atcgccttta tccgggtgat tatccgttgc
      301 gtattgccga cctgggaacc attatccgcg gccaccaggt gagcgatacc tatttcgccc
      361 tctcaaccgt aataggtcat ctcattgagc gaaagatcgt cccggtggtc atcggcggca
      421 gccaggacct gacctatgcc gtttacaagg ctttcgaaag cctgaagcgc atcatcaaca
      481 tcgcggcggt ggataatatg tttgacctgg gcgagggcga agcggaggtg aactcgcgca
      541 attacctgag ccatatcatc cttcacaagc ccaattatct gttcaattat accaatatcg
      601 gctatcagac ctattttgtt gatcaggggg ccattaccct gatgaagaac ctgtttttcg
      661 atatcaacag gctgggcatg gcgcgcgcca atctgcccga aatagagccg ctggtgcgga
      721 attcggatgt gcttactttc gatatttcgg ccgtccggca aacggacgcc cctggcaacg
      781 gcaatgccac gcccaacggg ttttacggcg aagaagcctg ccaaatcatg cgttatgccg
      841 gcttaagcga gaaactttcg gccatgggca tttttgaagt aaatcccgct ttcgaccggc
      901 acggtcagac ggaacacctg gttgcccaga tgatctggta cttcatcgac ggcttttacc
      961 accgccagga tgatttcccg agcgccgaat ccgacgactt tatccgctat accgtgcagc
     1021 ttaccaacca cgaagaaggt atcatctttt ataaaagcaa gaagacggaa cgctggtgga
     1081 tggaggtgag gtgcaccgaa gatatccggc ataagtatct aaggcattat attgttccct
     1141 gcacttacgg cgattaccaa acggcatgca gcgacgacgt ccctgaccgc tggtggcagg
     1201 cttaccagaa actgatgtaa agaaaagagg ctgccttctc acttttaagg cagcctcttt
     1261 ttgtatgaat accggtttta ataccggaca tctccccaga agggcggttc ttcatcggcc
     1321 cgtcgatcgt cgaagtgctt cttaaggacg gtcaggtact gctcttcgct gactttttta
     1381 cccttcattt tcttaggcgg gttcaactcg tcggtgagcg gtttcatttc caccttatcg
     1441 gcggttatca acatggcacc gtcgttaata tccacttcga gtatcacccc cggcagtccg
     1501 ccaagtcttt cgggaccggc gcttaccggt aagtcgagag cataccaggc ggttatcttc
     1561 tgcattttga gggtatcctc gtaaaaagca ttcatgcaga tatgcccggc tacttctttc
     1621 atatcgttga ggattttcca ctgagggtaa ttcagcgtat cttcgatcac atacacttta
     1681 ccgtttaact ggatagcgtc gtggatacgg gaggtttcga aattgcgcat gatcaggaat
     1741 acttccttgc gccagtcgta gcccccttcc ctgtcgcggt cggctatttc ttccgaatcc
     1801 tcatatttgg agccggccgg tgagaaccac aggttggtaa aggatttata ttccgcgcgg
     1861 ctggaccaca tgtacataaa ccggtcgcgc tgctgtttgc taaggtaatc aacggcggcc
     1921 atctgtttgg cccagttgcc ggtccgcagg tagcggatat agccttccgg tttttgggcg
     1981 atgccgtcga tggtcagcat gatcaggagc agggctgtga tcatgcctgt gaaatgaaac
     2041 cgggatgtat ggtttttcct gatgtcggtg ttataaatca tggcttaaag tgctggagat
     2101 aattcttaat tagatcagaa catattgttt ttactcagct tgtcatcgta accgcggaga
     2161 ttataactga ggctcagcag gaagtagcgc gccagcgtag gcgtcatcga attcgtgaca
     2221 taattggatg agccgtactg tgaaatattc acccgcttgt tgaaaatatc gaatgcagcc
     2281 aggcgcattt caaaacggtt tttcgtgccg agtatcctcc gtaacgaggc attcagaata
     2341 gggatcctgc ggtcgaaatc aaaacggtcg ttcacataca tggagtaact gaaactgctt
     2401 tcgaggaagg tctttttcat aaactgccac ttgagcgaag aatatacgga atggttctga
     2461 atattctggt tctgttcttt ctgtatggaa tactctatct tattataacc caggtttccg
     2521 cgcagatcaa ggatcagttt tggggaaggg gtgatgttaa agcctaaccc gatattaaac
     2581 ccattgtcgt tattgtcgtt gcggatgtca ttgacatacg acgccgattt gccggccgaa
     2641 taaccgccat aaaggcttgt tgaaaattta gttttgatga tcgggaaact ggaccagaag
     2701 ttaacattca aaccattccc tccggaaatg ttcgaaggcc tggtgatggt ctgaagcccc
     2761 acactatcaa cagtttttat ccactggttg taaatgatct ggtcttcata gatcgagtag
     2821 gaggaatata tgcctacgct ggcaaaagag gaaggattcc agtaattgta gctgaaattg
     2881 agactatgtg agcgttccgg taataaatcg gggtttcctt ctgtaaggaa attacggtta
     2941 tttgaattgg gaacagcctg caggtcgctt gccgaaggtt cgttgatgtt gtatgaatag
     3001 cctaacgaca tatacatatt gttaggcaac tgataattca tgcttagatt cggaacccag
     3061 ttggtatagg attgggtgaa gttcagtgtc gtatcctcta agggttttgc ccggtattgc
     3121 agatactcgc catccagctt aagctgctgt aatgcaacgc ctgccgatat gtttaaaccg
     3181 ttatgggaat acctgatgcc ggagccgata cggttgtaca gggtattgtt cttcgcattt
     3241 acactgacgc tgtcgatacg cgtatttcct tcggggttat taacctgccg gtcagttaca
     3301 ctttccgact ggttgaaatt atagaaggtt tcccagaacc agctttttaa aatgggttcg
     3361 gtgtacaatg cgcttgcttt tacctggctg acgtcggaag atttatcgtt caactgccgg
     3421 atctgctccg taaatgtcgt cgcggtgaaa aattcattga gcgagtacaa ggaatccaga
     3481 ttggcgctgt gggtgttatt gtaccctgca ctcagggcca gcgaccgtcc ttttttcagg
     3541 aacctgtgcc ggtaaatggc ggcagtggtt aacgtccagg aatcgagttg cgtttcgttg
     3601 tcggttgaaa gattgtttag cggagtatcc acccccgttg cgaaatgctg ccatcgttcg
     3661 ttggaagtat tgttgttgct gaacttgata ttggctttaa caataacttt gttattggag
     3721 tcgatgtcgt gttcaaaccg tgaaccaata ctgtgattat ccagaaaatt attttgtgaa
     3781 acggtatcgg tatttaatac ggtacccccc tcgaggaatg tttgtttact ggtatattgg
     3841 tctaagtcct gccgggtttg gttataaaag tagctggcat tgaatttcgt cttcttattg
     3901 tcgaaattat agttagtccc gccgccatag tttctgctgt aaccgcgccg gtccgatccc
     3961 atgaaggaat tgtcggtaat aataatggtg cccgaaccac ccccttcaaa tccaaaatca
     4021 ccattgtcgt aatcgttaaa ggtattctgt cctttaaact ccccgtaatc ctcccagttg
     4081 acgcccgatt ggttgatatt gttgccatag ccgataaacg acaactggtt tttctcgtca
     4141 aaacggttat aactgcccct tgcagcccat cgttcggagg tgcctcctcc cagggtggct
     4201 ttgccgaacg atcctttttt atattcctct ttcaatccga ggttcatggc tttttccttg
     4261 gttccgtcgt caacgccggt cagcttggtt tgttccgatt tttcgtcgaa tacctgaact
     4321 tttgaaatgg cttcggcatc aaggttcttg gtcacgcttt tcgggtcgtc gccaaagaat
     4381 gttttaccgt caacataaac gcgtttcaca tcttttccct gggtctggat gtttccgtcg
     4441 gcgtccacgt caattccggg caggcggcgc aacaaatcct ccaccgttga tccgggcgga
     4501 attttaaagg tagtggcatc atattcaatg gtatcgcccc gtatgcgcaa cggcgccttg
     4561 gcagctttga tgaccacttc cataagggtt tgtgaaatgg gtttcagcct gagaatcccc
     4621 aggtcgttga tttcggtaga cgacggttca agggctttct gataaggaat gtaactgaca
     4681 tgggatattt tcaggaggta cggaatattg cgtacgccat tgaatgaaaa tatgcctttc
     4741 tcattggttg ttgtgaaatt taccagggtg gagtctttac gattcaggag catgaccgtg
     4801 gcataaccgg cttcggcatt ggtcgaatcg cttgcaatac cttttatggt aacttttgaa
     4861 ggatgctgag ctgttaaaaa ggccggaaaa cccagcacaa acagcaggag ggcaacatgg
     4921 aattttctca taaccgatgt taaaagtaac aactaagttt ttagttctgc ttataaacgc
     4981 atcaaaggtc ttcaaaattg tacaggtggt tatattaata attgttaaaa gtctttatta
     5041 taatggaatg agcataaacc tgtcactgca aaataaggaa ataatttcga taattatcag
     5101 ataaacaata aataaagtct atttcaccct gtcgggattg ttgctgataa aagacgacca
     5161 gcctgaataa tttttcccgg tttcggaagt attacgctgg aaatagtggc aggtagccac
     5221 agcgacggca tcggtggcgt cgaggtattt tggttgttcg gcaataacaa acatgcggtt
     5281 gagcatggcg gatacctgtt ccttcgaagc gttcccgtga ccggtgattg actgttttac
     5341 ctttcgcggc gagtattcaa agatagggat atcgcggtac aaggcagccg ccatggccac
     5401 gccctgggcg cgtccgagtt tcagcatgct ttgaacattt ttcccgaaaa acggagcttc
     5461 cagcgctact tcatcgggat ggtaatgatc gacgagcgcc agcaccttga cgaatatgtt
     5521 tttgagtttc agcggctggg tatcgaggtg gtcgagtttg atgacatcaa gtgcaatcag
     5581 gtttaattgt ttacctttaa cctcaattat cccgaaaccc atgatgttgg ttcccgggtc
     5641 aataccaaga ataattttgt cagattccac tttttgtatc cttaaacgac cagcaatccc
     5701 agccttgaac cgatcaaagg taaataaaac gctcaacatt tttctccggc ttctcatcct
     5761 tgccgtttcg ttcgggtata tcggcctgaa gatcttcggc gaaaggggaa caatcaacac
     5821 ggatgttatt cttcaaaacc tggtcagcca gccgtatgtc gtccctttca gtattttgct
     5881 gtttctcctg atgcttgcca actggtcgct cgaaaccctg aaatggcggt tcctggtgag
     5941 taaagtggag aacgtgggtt tcgggagcgc ttgtgttgcc gtactcacgg ggataacggt
     6001 gagcattttc acgcccaaca gggttggaga gttcttcgga agggcttttg tcctgcgtaa
     6061 ggccaatcct gccaaagcca ttttgctgac ccttgtgggc agcatgagcc agttgctggt
     6121 gaccgtgatg gccgggtcct ttgcccttgc atttttcctg ccgaagtatg ttccgttctc
     6181 cgggatgtgg agttccggca tatacctttc agtggtcgca ggaatggcag tggtgaatct
     6241 tgtttttgtt cttttctatt tcaacgtacc cttgctggga tcgttttctg cctggctggc
     6301 agccaaaaat ttttcacgtc tttcgggtta tctccgcgtc ctcaatgatt tttcgcggaa
     6361 agaactgctg aaagcgttgc tcttcagcct gacgcggtat gtggttttca gcgtacagtt
     6421 tttcctggtg ctgttgttgt ttggcgtgcc ggtgacgccg ttatcggcag ttcttgccat
     6481 cccggtcata tacctggccc tggcggcgat tcccacagtt gctctttcgg aactgggcgt
     6541 tcgcggatcg gtagccctgt ttgtcctggg aatggtgatt ccgggcggcg ccctgaacca
     6601 cacgattcag gccggacttt cggcagcgat cgtatcggcg gcaacagcgc tttggtttat
     6661 aaacctggcg gttccggcgc ttgccggcat gttatttatt taccgcctga aatttgtacg
     6721 gcaatgacct ggatcatcgt gactcttgcc ttgcttgcct tatatgccgt cctgatgctg
     6781 atgatcacgg caggatggtt cagcatgccg gtatttcatg gttccggcgc ggaaccggta
     6841 aaaatatcgg taattattcc cgtgcgaaac gaggagaaac acctggcggc gctgatcggc
     6901 gatatgatgg cgcaggatca tccagcgact ttgtttgaac ttatcattgt tgacgatcac
     6961 tccggcgatc ataccagcga ggttgttcac tcattcatgg aaaactttcc cctggaaagg
     7021 atcagctaca tgaacctggg cgatttcccc ggccgggaag gcaagaaaga cgccattgaa
     7081 accggtgttg aaaatgcatc gggaaccctt attattacga ccgatgccga ttgccggttg
     7141 ccaaagacct ggctctcaac tgttgcctct tactattccg tttacaagcc gaagcttttg
     7201 ctggcgcctg tcaggatgtg tcccggcaat actttattcg gacgtcttca atcgctggag
     7261 tttaccagcc tgatcgcggc atcggcaggc tcggcatcgg cagggttccc gttgctggcc
     7321 aacggagcca acctggcatt tgagaaagaa gctttttttc aggtgggcgg ctattcatcc
     7381 aacaggcatt atccttcggg cgatgatatt tttttgctgc aatccatgag gaaacaattc
     7441 gggccaaagg ctgtgcatta catcaaatca aagctggcga cggtttcaac gcctgcgcag
     7501 gcaggtttaa aggaattcct gaaccagcgc ctgcgatggg tttcgaaaaa cgggggatac
     7561 cgcgatatga acgtactgac ctcgggatcg ctggtttacg ctgtgaatgc gttgttgcct
     7621 gccctcatgg tggcgggatt ctttaacggg tttgcctgga aagctgcctg catttggttt
     7681 atggtaaaac tggcagttga tttcccgata ttggcaggcg ttacctcatt ttccgggctt
     7741 ggaaaaacca tcctgctgct cccgttgatg gaaatactga attcacttta tacgacgttt
     7801 atcggtttcg ccggattggt cattccttat gaatggaaag ggaggaaatt caatagctta
     7861 taaaggttta gcggcagttt atcaacagcc catccggaag cagatccaac ggattaaatc
     7921 agcccgaatt cagcttaagg attagtaata ccatcaacta aaccctttgc gagggaagtc
     7981 acgatttagt agctgttgtt aaatattggc atgcggtctt ataaacgaat gcccatttgc
     8041 tattgctgaa tctgggaaag gatttatcag cataattaaa ttattttgaa agcgccagac
     8101 tccaaacgag gggaatagac tcaactaata tgttccaaaa aatcagaaaa ttttgatttt
     8161 tcggaaccta gatcgcccca tgtgactcaa agaccttttc taatggatta gcatattttc
     8221 tcagtggctc ttcagtggcc tcagtgccca ggtggttgcc ttctctttta ccattaagac
     8281 acttagaaca cccaggggta ctcaggattt ttttaccacg gagccatgga gaacatagag
     8341 caatgtattg tttctcagtg aaccttcgtg gcctcagtgc cttggtggtt cccttctctt
     8401 tctaccactc agacacttag aacactcagg ggcacttagg atcttttacc acggagccac
     8461 ggatcataga gcaatgtatt gtttctcagt ggaccttcgt ggcctcagtg ccttggtggt
     8521 tcccttctct ttctaccact cagacactta gaacacccag tagcacttag gatctttaac
     8581 cacggagcca tggagaacat cagagaacac agagagtagc atattttgtc agtgaaattc
     8641 agtgccctca gtgccttggt ggttgccttc tcttttacca ttaagacact tagaacaccc
     8701 aggggcactc aggatctttt accacggagc cacggagaaa atggaggaat acggagaatt
     8761 gcatattttc tcagtgaacc ttagtggctt cagtgcctga gtggttctct tcttatttta
     8821 ggaatagatg atttaccccc taagtagcca acgcctggtc tactcgcctg tcttccaaag
     8881 aatcaggctg tcgacggcag cttcgttgat ttcgccgttc atattttcgt tggcaggctc
     8941 aaataccagt tgcagggtat gtttcccttt atccatcaaa acgacgacgg cattggaata
     9001 tccccagtta gaccattctt cttttccacg ctgcgggaaa acaaaggttc ccttgaattc
     9061 cccgtcaagc tgtaatgtcc tgacggcgca tttattctcg gtatttacgg gtccatttcc
     9121 gttagcatac cggaacgata ccgcataaga tcctttttcc tgaatttcaa ctgtcaccgg
     9181 aagggtgagg ttttcagtcc tgctgatgat gacgtaacca tcgccggtat agtttttata
     9241 ccgggtggcc acgggaccgg caaagttttc aggattataa acattgcaca taccggtatc
     9301 ggcaacaacc acaggttcgg aagcaaagga ttgaaccttg tttttatcaa ccgctatcac
     9361 ctggtattcc gaatattcct tcttttccac cggaaaagca cctgctgatg tgtgtgcggc
     9421 ggccttcccg tttttcagca caaggtactc aactgcattt tctgctgcat tccagcggag
     9481 ttcattatgt tcgtatttca gcactggcgc ttcaggagag aatgcaacag gacatttgtt
     9541 gatcggcgca tcatcagaaa agccatccaa tatgatttct acctgatgtc ttcccatgag
     9601 ggaatccgga atgatatacc ggttgctttg tattccgtcg acagagaagc tgcgaatcct
     9661 gttcccgtaa ccttccattt cgatatcgag aatcatgccg cgatacctga aacccgataa
     9721 tacccgtttg ccgttgagcg cctgcggaac gaaggggtga aacacgaggc tgtcgggctg
     9781 aaattcgatc ccgaacagca ctttatggac cagcgcgatg ctgccggaca aactccagag
     9841 catgttgcct gaatttatct gtgtgccggc ataatcgccg gttgaggcga caaagttttc
     9901 tttgttggtc aggaacagcg ctgcaggccg gtacacggcg gcgattgact caagcaccga
     9961 cttttcgttc ccggccttcg ccgatgctaa cgcccagtaa gattgcacga aaggccagac
    10021 ggcattgttg tggtaaggcg ggatgcctgg tatttggggg tatatgcagg aaatgccgta
    10081 atccgttacg ggaacgttgc ggataaccga tttcccggtc tgttcatcgg caatgccgta
    10141 caaaacacac aaagcttcac cgagggcttc ggcccggggc gacaaagaac tgaaattacg
    10201 accgtagagg tactgaccgt agtatccttt ttcgggcatc cagaggcagc ggttaatgcc
    10261 atccctgatt ttttctgcgt tatggttata ccgttccgtt gattttttat cggagaggcg
    10321 ttgggccatt tctgctaaaa tccggttggc ctgataatgg acagcattgg tacccagatt
    10381 ttcggaattg aaaatgtcgg ccggctgcat ccacctggga taggtttgtt cgcgccagtc
    10441 gaggaaggaa gattcccccc tgaccagtcc ggtttcagga tcataaatat tcagcatgtc
    10501 atcctccaca gaattccgga tgatgccgta agcctgcttc agccagtctt cgtcgccggt
    10561 ggctttgtac acttcccatg cagccgcagc ccagatcatc ctgtcggaag atacgggata
    10621 ggcccctccg gtaccggtat cctggatgat cctgccgttt ttcacttttc gcatcaggct
    10681 gtacatggca acctgcggct gcaggtaggc catggaaagg atgatgctgt aactgatatc
    10741 ccgggtccat acccctgccc attccttccc tgtcctgaaa gtgctgtcag gttccactgc
    10801 ctttatcatc tcttcgatag ccaggttata cagcgcgtcg gaaatcaggt aatccgaatg
    10861 gtaaaccgga aaagaggtga tgtccttcga caatttccag gatgctgccg ttcgcttgct
    10921 gttatcctgt gagttcatga ccagcgtggt ttcaaagatg ccgtcatgat caggatcagt
    10981 aagctgcaaa ccgggttggg tatgcaggtt attaaaatcc cagctcagcg gggctgcgtt
    11041 tccggcaata taaacccctt taaaatcatc acggaagagc ttttcaccat tgaagaaggt
    11101 ataaaatccc tgctttttaa aagcatcgaa cacttcgcgc atgtcaagcc ttatggtcca
    11161 cagggtattg ggcttcaggt ttttgccttc ggcgaccttc accgtatgca catattgttt
    11221 cccaaaacgg actgtagtgg tacaatgtcc gttgaccggc tgcagcgtga ctttatgatc
    11281 acggcccgaa ggcatttcgt tatcacgcag gttgatgctg aatttaaaag tgatatcggg
    11341 actgtaacag ccatcccggg gacttttata atctgaagtt atctcttcag ccgaccgggc
    11401 gacagcggtg aaaccgccct gcacaaccct gtcgtggtaa acggaatagg cttccgaacg
    11461 gtaaacaggt ttctcttttt cctgtccgga tactatcaag gttccaaaac aacagaaaca
    11521 ggcaagaaat atagttggtc tgatcattaa cgggagtttt taacagtaat ttcagtcatg
    11581 tccttcggtt ccgggaattt tatccagagg gttttacttt tcttatcata ggaccattcc
    11641 ggtttttcgg caacagcata ccatctttcc cggagtcttg catcggcaac ctgttttagc
    11701 ttgtttccgt ccattgcaac acttgcgggt tccttatccg tgtaaattct gatcccgaag
    11761 ttgcgtttat ccccgtgata tccgccgttg aaatctttct tttccaggga aagtgtgata
    11821 tcgccgtttt taaaactgca tttaacacgg gtaagggaat agacatcttt tttataatcg
    11881 ttgctctcgc cgtcatcgtc gtaaagttca gcggacgatt taccggcatt cttcgggaac
    11941 accgtgaacc ataccggtgc atcgggtttt tcgtgtatat attgcaggac cggcatcatg
    12001 ggaatgaccg aaccggatct gacaaataca ggaatggttc caagtgctac cgggtaatca
    12061 atccattggt ttcccgtgaa gcggctggaa gggtcgttga aacaggccca ttccccttcg
    12121 ggcaggtaaa cttttaccga tgttgcattg ggctcaacca caggcgctac cagcagatcg
    12181 ctgccaaaca taaactggga atcaaggtta aaggtttcct tgtcgtcggg acaggccatc
    12241 accatagccc gcataagggg aaggccttcg tcgtgggcct ttcgtgcaca accgtacagg
    12301 tagggaaaca gggagtattt caggctgatg gcatccttgc aggctttttc ggcttcgggc
    12361 ccgaagagcc agggttcgac ggcattgttg ccttcatgat gggcgcggct gatcg
//
LOCUS       DAOPYV010000080        12373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_5729_length_12373_cov_12.755074, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000080 DAOPYV010000000
VERSION     DAOPYV010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12373
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5729_length_12373_cov_12.755074"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..1561
                     /locus_tag="PKI35_09855"
     CDS             398..1561
                     /locus_tag="PKI35_09855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX44729.1"
                     /translation="MNEKIWNRDFIFLVLSNFLMYITYYAILSALPIYLVNDLHASKS
                     QVGVVVGAYTIASVLVRPFSGFTLDRFGRRTIFLLALIIYAFLFAGYLVAFTILTIML
                     LRFAQGITWGFTTVSGSTVAADIVPVSKRGEGIGYFALSTTLGMSVGPVIGLFVCNQW
                     GYLALFVTGCLVSLASLACAYAIRMRKRFVVGRRIQFKWDSLFDKNSVRPSLNVFITM
                     ISYGALLSFIALYGREIGIRNSSLYFLIFSVGIAAARLTSGKEFDRHGPRRIITLCII
                     LLMIGFPLLAMARNATLFYLSAIIIGFGNGVIFPTFQSMVNNLADTEHRGAANSTLYT
                     AVDLGMGLGMISGGLIAQHVSMSAIWWISALVSAAGLLFFRGFVIKYYERSTR"
     gene            complement(1717..2394)
                     /locus_tag="PKI35_09860"
     CDS             complement(1717..2394)
                     /locus_tag="PKI35_09860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNX44730.1"
                     /translation="MSGRLFAIGDIHGCFEPLKALIEEKIRIRKTDKVVFLGDYIDRG
                     PQGKEVIDYIMRLPDKGYDVIPLSGNHEAMLLDAYADQGNLPLWIYNGGYTTLMSFHI
                     RSIRELSDKYLEFFRGLKYWHQTRKYIFVHAGFNDELEDPFTDKYAMIWECRHHYENP
                     VLKDKVVVHGHRAVPAEICKMSLKESFPVIDVDTGCVYRNFEGFGRLTAVELYSKRCF
                     IVGAADA"
     gene            complement(2629..4026)
                     /locus_tag="PKI35_09865"
     CDS             complement(2629..4026)
                     /locus_tag="PKI35_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNX44731.1"
                     /translation="MKISARSFLFIAFSAITAVILFNIGAFYDIWPAGNMLLAASRWS
                     GIAVLVWYAFRRRSLTSWILVSMVAGAEFGYDLPAIAVHLNICSKIFLRMVKTIIAPL
                     LFATLVVGIAGHSNLRQVGRMGWKSIVYFEAVSTIALFIGLFAINWSKAGTGVVIPPE
                     IAQQTVPEVKAHSFEDIVLQVFPENIAKAIYEGQVLQIVIFSIIFGIAVAMLREDHRR
                     RMVNFAESLSEAMFKFTNLVMYLAPVAVFAAIAYSVGHMGLGILGNLFKLLATLYVAL
                     FLFLLGVLLPVALILKIPVKNFVRAISEPATIAFATTSSESALPIAMEAMEKFGVPRK
                     IVAFVIPTGYSFNLDGTTLYLSLATVFVAQVAGIHLSFDQQLLIVFTLMLTSKGVAGV
                     PRASLVILLGTAASFGLPAWPIFIILGIDELMDMARTAVNVIGNCLATAVVARWEKEF
                     DEDAAKQFTTRIIEK"
     gene            complement(4133..4666)
                     /locus_tag="PKI35_09870"
     CDS             complement(4133..4666)
                     /locus_tag="PKI35_09870"
                     /inference="COORDINATES: protein motif:HMM:NF025425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HNX44732.1"
                     /translation="MKTICLLTAAILFSISVSAQETGKQKKSREEKAAEMELKFRETQ
                     NLLDSACFVLEADYLGNGFYRVPVLSTINFIMVDSASSVIQTGRNFGLGYNGVGGVTV
                     EGSITKYTVRHDNKRKSFYVSWNVSTAIGFYYISMQVSASGRATATLTGNSAGRLIYE
                     GWLVPVPASGIYKGSRI"
     gene            complement(4981..6054)
                     /gene="buk"
                     /locus_tag="PKI35_09875"
     CDS             complement(4981..6054)
                     /gene="buk"
                     /locus_tag="PKI35_09875"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002834.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNX44733.1"
                     /translation="MKSILVIYPEPDITKIAVYSETNLVFLKSVRHKPEDMAAFADFA
                     GQLDYRTGRIMDELNDNQIELQDIAVVMARGGLIKPVKSGVYQVNEAMKRDLTTGVMG
                     SHAANLGGLIADKIASLLPGAGAYLADPVVVDELEPIARISGLPQIERTSIFHALNHK
                     SISRMYAASINRKYEDLRLIVAYIGSGGVSIGAHSGGRVIDVNQAFDGDGPFSMTRSG
                     SLPVGQVVDLCFSGKYTRDEIRKLITEEGGIAAYLGTKSLSKVLELIEEGDEHAMFIV
                     GAMIYQIAREIGAVATVLEGRVDAILIMGAIMNSRFFSEGLLKRIKTIAPVSVYTVVN
                     DLDSLAANGIAILRGEAQIIEYK"
     gene            6108..7073
                     /locus_tag="PKI35_09880"
     CDS             6108..7073
                     /locus_tag="PKI35_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNX44734.1"
                     /translation="MKGLHSRTHTGGYGIFFLIVSLRTMMNRMQMKRFLALLFSSLTI
                     SQVFAQRDTLLPARNLCRQGRWVLVFEDEFNYRNLDRKKWNTWFPYSEDGSDSCEFCR
                     THGAEGQVYSDANVITSGGTLKLIARREPATWYDSRREFTSGMIHSKQAFGYGRYEIR
                     CKLPEGNGFWPAFWMFGQKATEIDVLEAGTQKPRHLHLGIRTWANGNKFDKGLRSRTR
                     LTRGFHTYAMEWDENFIRFEVDSLEVWRITRYLDKRGKTLKRCEINAGRYRLQPAWPP
                     EGERLQIIVNLAIGTETTPFTKSPGEKTMLPNQMEVDWIRVYEKR"
     gene            complement(7625..8866)
                     /gene="pepT"
                     /locus_tag="PKI35_09885"
     CDS             complement(7625..8866)
                     /gene="pepT"
                     /locus_tag="PKI35_09885"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNX44735.1"
                     /translation="MKQQILDRFLRYIAIDTQSDDASTTFPSTSKQFDLLHLLRDELV
                     AFGLQDVVLDEFGYVMATLPANTGKKVPTIGFIAHVDTAPDMSGANIRPRLVGNYDGS
                     DIVLNSDLNIVLSPSEFPEMKEYIGQTLIVTDGTTLLGADDKAGVTEIMAAVEYLVRH
                     PEVEHGEIKIGFTPDEEIGRGVDKFEVERFGADFAYTMDGGGVGELEYENFNAAGAKV
                     FIQGRNIHPGYAKGKMKNAILMAMEFNALLPVSERPEFTQDYEGFYHLVKTEGSVEHS
                     MLQYIIRDHDRHKFEMKKEAFKACIDFMNRRYGENTFTLEMKDQYYNMREKVEPVYHV
                     VSTAQEAMELTGVVPKVVPIRGGTDGARLSYMGLPCPNIFAGGHNFHGKLEFVPLESM
                     VKATEVILKISELYTQRGEKA"
     gene            complement(8919..10904)
                     /locus_tag="PKI35_09890"
     CDS             complement(8919..10904)
                     /locus_tag="PKI35_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HNX44736.1"
                     /translation="MSDHETGPIKGLPENAYKELKEGESYEPIMSPHKTYAEVNTWSV
                     LWGIVMAIIFSAAAAYLGLKIGQVFEAAIPIAIIAVGLSTVFNRKGALGENVIIQSIG
                     ASSGVIVAGAIFTLPALYILNLEAQFYQVFLSSLLGGFLGILFLIPFRKYFVSEMHGK
                     YPFPEATATTEVLVSGAKGGSQARLLLVAGLIGGIYDFVIATFGWWAEVFTTRVVPVG
                     QMLADKSKIVFKLNVGAAVTGLGYIIGLKYAAIICAGSFVGWYVIIPIINYFAGGQTI
                     AVGANITALISQMSAEQIFTAYIRPIGIGGIAMAGIIGIIKSSKIIKSAVSLAGKEIF
                     GKHEGTKTDLRTQRDIPMTIIVGGILLTALIVFIFFQFGVVHNLTHALVGLAIVMLIA
                     FLFTTVAANAIAIVGTNPVSGMTLMTLIISSLILVSVGLKGTSGMIAALIIGGVVCTA
                     LSMAGGFITDLKIGYWLGSSPYKQQTWKFLGTLVSAVTVGGVIMVLNKTYGFTGENAM
                     VAPQANAMAAVIGPMMSGQPAPWMLYAAGAFLSLILTMIGVPALAFSLGMFIPIQLNT
                     PLLLGGLIAHFVGNRSRNEKLNQARRERGTLIASGFIAGGALMGVISAILANFGINFV
                     NKAWFESHSAEALALLMLCLLCGYFIWECLRAKVEEE"
     gene            complement(11014..12273)
                     /locus_tag="PKI35_09895"
     CDS             complement(11014..12273)
                     /locus_tag="PKI35_09895"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HNX44737.1"
                     /translation="MNLYYTALVIFLVTYTGIIFTRLPKMNIDRPSAAFFGAVAMILF
                     GVLSFEEAIQAIDFNTIALLLGMMIIIATLELDGFFSLIASKTMSLAKTPDRLLVIIT
                     LVTGITSAFLVNDAVVMLFTPVIILICRSLRTNPMPYLIAEIMASNIGSAMTITGNPQ
                     NMLIGISSGINYGTFLWHLLPVSFIGLFITIAVIRWFYRKDLRFNPIPEDNTAGNENH
                     LFEYNFRSMNTSVPVFLLVVVLFFMSHRIGLSVPMIALLGGSLILIFGKIKPSLVIRK
                     VDWVLLLFFASLFIVVKGIEKVGIMDSLIHGVALKADAKGLAGIHLISLVLSQVVSNV
                     PFTVVMLPLMKTAGSELLWLALASASTLAGNATIIGAMANLIVIESAGSKGIKIRFVE
                     FFKTGIIVTLLTLAVSFLMLYLKFLAF"
BASE COUNT         3228 a         3127 c         2970 g         3048 t
ORIGIN      
        1 atgaggaatt cataggccgg tgatttattc ctgagcccat aatcaaaaat aatgataatt
       61 cgctgataag ttctgccgat tgcatgaaat gcatcgaatg ttactatccg taatgaaatt
      121 tcatttttta ttgggatgaa ccagatgata tcaggctgat tctttttcat tactaattgg
      181 gagccccggt tcacttattg aagtacagat tacgccgcac ccttgccact ctcctgaaga
      241 gaggggctac acctcttcca cgtaaaaagt aaccattagt attgatgtat tcaaacaaaa
      301 tcagctttta ccggcaaatc atcacaacac ggcaatacaa atcctgcttc agtattattg
      361 gtaattttgc ctgccgaaag attctgaacc cggagctatg aatgagaaaa tctggaacag
      421 ggattttatc ttccttgtct tgtccaactt tttgatgtac atcacctact atgcaatcct
      481 gtcggcatta cccatatacc tcgtcaatga tctgcacgcc tcgaaaagcc aggttggcgt
      541 agtggtcggg gcatatacca tcgcctcggt gctggtacgt cccttttcag gatttacgct
      601 cgacaggttc ggccgccgca ccatcttctt actggccttg atcatatatg cttttctgtt
      661 cgcagggtac ctggttgcct tcacaatcct gaccatcatg ttgctccgct tcgcgcaggg
      721 catcacctgg ggatttacca ccgtttcggg ctctacggtt gccgctgaca ttgtgcccgt
      781 ttcgaaacgc ggcgaaggca tcggttattt tgcattgtcc accacgctgg gcatgtccgt
      841 aggaccggtg atcgggctat ttgtatgtaa ccagtggggt tacctggcct tgttcgttac
      901 gggttgcctg gtgagcctgg ccagtctggc ttgcgcctat gccattcgta tgcgcaagcg
      961 tttcgtggtc gggaggcgga tccagttcaa atgggacagc ctgttcgata aaaactccgt
     1021 ccgcccgtcg ctgaacgtct tcatcacgat gatctcctac ggcgcgctgc tttcgtttat
     1081 cgccctttac ggacgcgaga tcggcatccg gaattcctcc ctttacttcc tcatcttttc
     1141 cgtcggcatt gccgcggcaa ggcttacctc cgggaaggaa ttcgacaggc acggcccgcg
     1201 aaggatcatt accctgtgca tcatactcct gatgatcggc ttcccgttgc tggccatggc
     1261 gcggaatgca acactattct acctttcagc tatcatcatt ggattcggca acggcgtgat
     1321 atttcccacg ttccagtcga tggtgaacaa cctggccgat accgaacacc gcggagccgc
     1381 caattccacg ctttacaccg ctgtggacct cgggatggga ctgggaatga tctccggcgg
     1441 attgattgcc cagcatgtct ccatgtccgc catatggtgg atcagcgcgc tggtcagtgc
     1501 ggccgggctg ctgtttttcc gtggttttgt aataaagtat tatgagagga gtacccgata
     1561 acaaccgtat tggtgaaagg cagcatgttc cggaagcggg tctgatgatt tctttaacta
     1621 tggcttgaaa aggtcgggga atatttccgg caacgtgata ccctgatgtt tttgcccggg
     1681 aaacaaattt ttgttcagca atgaaagaaa ttctgatcag gcatcagccg ctccaacaat
     1741 aaaacatcgt tttgagtaca attcaacggc cgtaagtctt ccaaatcctt caaaattacg
     1801 gtaaacgcac ccggtgtcca catcaatgac cggaaaagat tctttcaggc tcattttgca
     1861 gatttcagcc ggaaccgccc ggtggccatg cacaacaact ttatccttca ggacagggtt
     1921 ttcataatga tgccggcatt cccagatcat ggcatacttg tcagtgaacg gatcttccag
     1981 ttcgtcgtta aaaccggcat gaacaaagat gtatttcctg gtttgatgcc agtatttcag
     2041 tccgcgaaag aattcgaggt atttgtccga cagctcccgt atcgaacgga tatgaaaact
     2101 catcagggtt gtgtatccgc cgttgtagat ccacaaaggc aggtttccct gatcggcata
     2161 ggcatcgagc agcatagcct catggttgcc cgaaagcggg atgacatcat agcctttgtc
     2221 cggaagtctc atgatgtaat cgatgacctc tttgccctga ggtccgcggt cgatataatc
     2281 gcccagaaaa accaccttgt cggtctttct gatccggatc ttttcctcga tcaaagcctt
     2341 cagcggttcg aagcacccgt gaatatcccc gatggcaaaa agcctgccgc tcatgatcat
     2401 agattttaaa tcgggtcgga cgtacccaac cgctgttgca tgactcaata tgtattttac
     2461 tcctgttgca ttgtcagggc cggaaaccgg gtgcatgtat tgaacttccg agaattaagg
     2521 aaaccgtaag acgaatggcg gggaggattc cggaaacatc tccaacaacg gatcgccttt
     2581 ttcatttttt agttaaggaa ggcgggaaac ctgcaataaa gttaatgctt atttctcaat
     2641 aatccgtgtg gtgaactgtt tcgccgcatc ctcatcaaat tccttttccc acctggcaac
     2701 gacggctgta gccaggcagt tgccgatcac gttcacggca gtacgggcca tatccatcag
     2761 ttcgtcgata cccagaatga taaagatagg ccatgccggc agtccgaaac tggcagccgt
     2821 acccagcaaa attaccagcg aagccctcgg cacgcccgcc actcctttgc tggtgagcat
     2881 gagggtgaat acgatcagca actgctggtc gaaggataag tggatgcctg ctacctgcgc
     2941 cacaaaaacg gtggccagcg aaaggtaaag cgtggttccg tcgaggttga agctgtaccc
     3001 ggtgggaatt acaaaagcaa cgatcttacg cggcaccccg aatttttcca ttgcttccat
     3061 ggcgatcggg agggccgatt cggaactggt agtggcaaag gctatggtgg cgggctctga
     3121 aatagcacgg acaaaattct ttaccggtat cttcagtatc agggcaaccg gcagtaatac
     3181 gcccagcaga aacaggaaca gcgcaacata gagcgtagcc agcagcttaa aaagatttcc
     3241 gaggatgccc agtcccatat gccccaccga ataggcgata gcggcaaaaa ccgcaacagg
     3301 ggcaagatac atcaccaggt ttgtgaattt gaacatggct tccgagaggc tttcggcaaa
     3361 attcaccatc cgccggcggt gatcctcgcg aagcatggcc acggcgatgc cgaagataat
     3421 gctgaatatc acaatctgta agacctgccc ttcataaatg gccttggcga tattttcggg
     3481 gaacacctgc aacacgatat cttcgaacga gtgggctttt acctcgggaa ccgtctgctg
     3541 cgctatctcg ggagggatca ctacacctgt ccctgctttg ctccagttaa tggcaaacag
     3601 tccgataaac agggcaatgg tggaaaccgc ttcgaagtac acgatggatt tccatcccat
     3661 gcgccccacc tgtcgcaggt ttgaatgccc ggcgattccc accaccagcg tagcaaacag
     3721 caacggtgcg atgatggttt ttaccatccg gaggaatatt ttactgcaga tattaagatg
     3781 cacggcaatg gccgggaggt catagccgaa ttccgcgcca gccaccatgc tgacaaggat
     3841 ccagcttgtg agcgaccgtc tgcggaaagc gtaccaaacc agaacggcga tgcccgacca
     3901 acgggaagcg gccagcaaca tgttaccggc aggccagatg tcataaaaag caccgatatt
     3961 gaacaggatg acggcagtga tggccgaaaa ggcaatgaag agaaaactgc gggccgaaat
     4021 cttcatccgt ttctgaaaag aaacaaatgt aggaatttct aagaccgcat ctttctataa
     4081 agtagtagat attacccagc ctgctccttc ggccagtcag cctgtcaaag gattaaatcc
     4141 tgctaccctt gtaaatgccg gatgcaggaa caggaacaag ccagccttca tagatcagcc
     4201 ttcctgcgct gttacccgtc agggtagccg ttgcccgtcc cgaggctgaa acctgcatcg
     4261 agatataata gaatccgatg gcggtggaaa cattccagct tacataaaac gatttacgct
     4321 tattatcgtg acggacggta tatttcgtga tagagccttc aaccgtcacc ccaccgacac
     4381 cgttatatcc cagtccgaaa tttctgcccg tctggataac agaagaggct gaatccacca
     4441 tgatgaaatt aatcgttgaa agcaccggca cccgatagaa cccgttgccg agatagtccg
     4501 cttcgagaac gaagcaggca ctgtcaagaa gattttgcgt ttcccggaac ttaagttcca
     4561 tctcggcagc tttttcttcc ctgctttttt tctgcttccc cgtttcctgt gccgacacgc
     4621 tgatagaaaa gagaatggcg gctgttaaca agcatatcgt tttcatggat ttgggttttt
     4681 gtacaaggat taaatcattt ctgacagaca gagagagtta attatttttc agacacctca
     4741 ccccaaccta tttcctcaag cagaggagca tttcttcatg gaggcaaggc ggcctcctcc
     4801 ctctcaagtc tttggctata aaccttgatt tattagccgt tcacgattat aattaaacac
     4861 aaaacccata ccacgcctct ccggatcaga atacttttca ggctgattta gaaaatttaa
     4921 cttaatgaaa acctatattt taaagatgaa atcacccatt ggtaatcaaa ccgccgttta
     4981 tcatttgtat tcaatgattt gcgcctcgcc gcgcagaatg gcaatcccgt tggcagccag
     5041 cgaatcaagg tcattcacaa ctgtgtaaac cgaaacggga gcgattgttt tgattcgttt
     5101 cagcaagcct tcgctgaaga agcggctgtt cataatggct cccatgatga gaatggcatc
     5161 aacccttcct tccaggaccg tggccacggc gccgatttcc ctggcaatct ggtaaatcat
     5221 ggccccgacg ataaacatgg cgtgttcgtc gccttcctct ataagttcca ggaccttgct
     5281 cagcgacttt gtaccaaggt aggctgcaat accgccttct tccgtaatga gtttccggat
     5341 ttcatcccgg gtgtatttac cgctgaagca caggtcaaca acctgcccca ccggaaggct
     5401 ccctgaacgt gtcatagaaa aagggccgtc gccgtcgaaa gcctggttca catcaatcac
     5461 ccgtccccca ctgtgcgccc caatcgacac tcccccgctg cctatataag ccacaatcag
     5521 tctcaggtct tcatatttgc ggttgatact ggccgcatac atacgtgaga tgcttttatg
     5581 gttcagcgca tggaatatcg aggtacgttc tatttgcggc agtcccgaaa tacgggcaat
     5641 gggttcgagt tcatccacca ccaccggatc ggccaggtag gcgcctgccc cgggcaacaa
     5701 agaagcgatc ttatcggcaa tcagccctcc caggttggcg gcatggcttc ccattacccc
     5761 cgttgtcagg tcccgcttca tggcttcgtt cacctgataa acacccgatt ttacaggttt
     5821 tatcaggcct cctcgtgcca taaccaccgc aatgtcctgt aattctatct gattgtcgtt
     5881 caattcgtcc ataatccggc ccgtacggta atccagttgg ccggcgaaat cggcaaaggc
     5941 tgccatatct tcaggcttat gcctgaccga tttcaggaag accaggttgg tttcgctgta
     6001 aacggcgatc ttcgtgatat caggttcagg ataaattacc aggatgcttt tcatgatgaa
     6061 tttcggataa cgattgacct atcaaagtta acacaaacac agtcagaatg aaaggattac
     6121 attcccggac tcataccggc ggatacggga tattttttct tattgtatct ttgagaacca
     6181 tgatgaaccg tatgcagatg aagcgatttt tggcattatt gttctcttcg ctcacgattt
     6241 cccaggtttt cgcgcagcgc gataccctgt tgcctgccag aaatttgtgc cggcagggaa
     6301 gatgggtact ggtatttgaa gatgaattca attaccgcaa ccttgacagg aaaaagtgga
     6361 acacatggtt tccttattcg gaagacggca gcgacagttg tgaattttgc cgtactcatg
     6421 gtgccgaagg gcaggtttat agtgatgcaa acgtgataac cagcggcggg accctgaaac
     6481 tcattgcacg ccgtgagccg gcaacctggt acgattcccg ccgggaattc acttcgggaa
     6541 tgatccattc gaaacaggcc tttggttacg gtcgttatga aatcagatgc aaactgcctg
     6601 aaggaaacgg attttggcct gctttctgga tgtttggcca aaaagctacc gaaattgatg
     6661 tactggaggc tggtacgcag aaacccaggc acctgcacct gggaatcagg acctgggcca
     6721 acggtaataa gtttgataag ggattacgaa gcaggacccg tctcacccgt gggtttcata
     6781 cttatgctat ggaatgggac gaaaacttca tccgcttcga ggtcgacagc ctggaagtat
     6841 ggcgcataac ccgatacctc gacaaacggg gcaaaaccct taaacgatgc gaaattaacg
     6901 ccggccgata ccggctgcag ccagcctggc cacccgaagg cgaaaggctg cagatcatag
     6961 taaacctggc catcggaact gaaacaacac cttttaccaa atcgcccggt gaaaaaacca
     7021 tgctccccaa ccagatggag gtcgattgga taagagttta cgaaaagaga tgagaatcag
     7081 tggttttacc ggtagagcag aaaaaaaact acatatttta acgttgttaa taaaaaatat
     7141 tcgggttgca gtagtggttt attgtttttg aattgtcata tacatataaa aacaatgtga
     7201 ccatttgaat tcatgaaccg tgaaaatttt gtcgctttta tcatattccg ttttctgttc
     7261 ctgccctatc ccttcaatca caggcgtcat tgcctccata gcatatatct atcgataccc
     7321 caaagcacgg ctgctttatg gcagcctccg caaccaacca gttatgatat gaaatgagaa
     7381 tttcgagggc aggatttttt ctttataccg gacacatctg cagtatacaa taaaaacaac
     7441 cattcattca tcctgaagaa agagaaaaac agggtatccg ccccggaagt aagggccgga
     7501 ccctttttgc ccggcctatg ccatagtaaa aactaatccc cggaagcaag gagggcccgg
     7561 aaacaaaaat cagttgtgat gtttatcagg taactgacgg tggaatatta aggcagatat
     7621 gctttcaggc tttttcgcct ctctgcgtat acaactctga tattttaagg atcacctctg
     7681 tggccttcac catcgattcg agcgggacaa attcgagctt gccgtggaaa ttatgccctc
     7741 cggcaaaaat attgggacaa ggcaacccca tgtatgaaag acgcgcgcca tcagtccctc
     7801 cgcggatagg aaccactttg ggaacgacgc cggtcagttc catggcttcc tgtgccgtag
     7861 aaaccacatg ataaacaggt tccacctttt cgcgcatgtt gtaatactgg tctttcatct
     7921 ccagtgtgaa tgtgttttcg ccgtaacgcc ggttcatgaa atcaatacag gctttgaagg
     7981 cttccttttt catctcgaat ttatgcctgt cgtggtcgcg gatgatatat tgaagcatcg
     8041 aatgttccac cgaaccttcg gttttcacga ggtgataaaa tccttcataa tcctgcgtga
     8101 attcaggcct ttcgctgaca ggcaacaaag cgttaaactc catggccatc aggatggcgt
     8161 ttttcatctt tcctttggca tatcccgggt gtatattccg gccctggatg aataccttgg
     8221 cgccggcagc attgaagttt tcatattcca gttcccctac tccgccgcca tccatggtat
     8281 aggcaaaatc ggcgccgaaa cgttcaactt cgaatttatc aactccacgg cctatctctt
     8341 catcgggggt aaaaccgatt tttatttccc cgtgttcaac ttccgggtgg cggaccaggt
     8401 attccaccgc cgccattatt tcggtgaccc cggctttgtc gtcggcaccc agcagggtag
     8461 tgccatcggt cacgataagg gtttggccga tatactcctt catttccggg aattcggaag
     8521 gtgaaagcac aatgttcagg tcactgttca gaacaatatc ggaaccgtca taattcccga
     8581 ccagcctcgg cctgatgttt gccccgctca tatcgggagc ggtatccacg tgcgcgataa
     8641 aaccgattgt tggtactttt ttgcctgtgt tggcaggcag tgtagccatc acataaccga
     8701 attcatcaag cactacatcc tgcaacccga atgctacaag ttcatcgcgc aacagatgta
     8761 aaaggtcgaa ttgctttgaa gtgctgggga aagtggttga agcgtcatcg ctttgggtat
     8821 cgatggcaat gtaccggagg aaacgatcga ggatttgctg tttcatataa ggatgagtat
     8881 tattatgctg tgaatttcaa tttctgcaca agctggtttt attcttcttc cactttagcc
     8941 cttaggcatt cccagataaa gtaaccgcat aacaggcaaa gcatcagtag agcgagcgct
     9001 tcggctgaat gcgactcaaa ccatgccttg ttcacaaaat tgattccgaa gttagccaga
     9061 attgcgctta tgacacccat cagtgcaccg ccggcaataa atcccgaagc gatgagtgta
     9121 ccacgttcac ggcgtgcctg gttgagtttt tcattcctgc tcctgttgcc gacaaaatgt
     9181 gcaataagtc cgcctaacag cagaggcgta ttcaattgaa tgggaatgaa cattcccagc
     9241 gagaaagcca gggcgggaac gccgatcatg gtgaggataa gcgacaggaa agcaccggca
     9301 gcatacagca tccagggggc aggttgcccg ctcatcatag gcccgataac ggctgccatg
     9361 gcattggcct gcggcgcaac catggcattc tcaccggtaa aaccgtaagt tttattcagg
     9421 accatgatca caccgcctac cgtgacggct gaaaccaggg tgcccagaaa tttccaggtt
     9481 tgctgtttgt aaggcgaaga accgagccaa tacccgatct tcaggtcagt gatgaaacct
     9541 cccgccatcg aaagcgccgt acaaaccacc ccgccaatga tcagggcggc gatcatgccc
     9601 gaagtgcctt tcagcccgac ggaaaccagg atcagcgatg atatgatgag cgtcatcaac
     9661 gtcatcccgc ttaccggatt tgtacctaca atagcaatgg cattggccgc taccgtggtg
     9721 aaaaggaaag cgatgagcat gacgatcgcc agtccgacaa gagcgtgtgt caggttgtga
     9781 accacgccga actggaagaa tatgaatacg atgagggctg taagcaatat tccgccaacg
     9841 atgatggtca tcgggatatc gcgctgggtg cgcaggtctg tttttgtccc ttcgtgtttg
     9901 ccgaaaattt ctttgccggc cagcgacacg gccgatttga tgatcttgct tgatttgatg
     9961 atgccgataa tgccggccat ggcaataccg ccgatgccga taggacggat gtaggcagta
    10021 aagatctgtt cggccgacat ctggctgatc agggctgtga tgttggctcc gacggcaatg
    10081 gtttgtccgc cggcaaaata attaatgatg gggatgatta cataccaccc gacgaaagaa
    10141 ccggcacaga tgatggcagc atatttgagc ccgatgatat aaccaaggcc tgttacggcg
    10201 gcgccgacgt tcagtttgaa aacaattttg gatttatcgg ccagcatctg cccgacgggg
    10261 accacgcggg ttgtaaacac ttcggcccac catccgaaag tggcgatgac aaagtcgtag
    10321 ataccgccga taagccctgc taccagcagc aaccttgcct gacttcctcc cttggcgccc
    10381 gataccagca cttcggtggt agccgtagct tcagggaagg gatatttgcc atgcatttcg
    10441 cttacgaaat acttccggaa tggaatcagg aacagaatac caaggaaacc tcccagcaag
    10501 gacgacaaaa atacctggta aaactgggct tccagattga gaatgtaaag tgccggcaat
    10561 gtgaatatcg ccccggctac aatcacgccc gaactggccc cgatgctctg gatgatcaca
    10621 ttttcgccca gcgcaccttt acggttgaaa actgtagaaa gcccgacagc gataattgcg
    10681 atcgggatgg cggcttcgaa tacctgccct atctttaatc caagatatgc ggcggcagcc
    10741 gaaaatatga ttgccattac tattccccac aaaacactcc aggtatttac ctcggcatag
    10801 gtcttatgag gcgacatgat aggttcgtag ctttcgccct ctttcagttc tttataggcg
    10861 ttttcgggaa ggcctttgat aggcccggtt tcatggtctg acatgagaaa aagtcttagt
    10921 ttgccttcaa atttagaaaa attaagaatt gtacacagga taagacagga ttcgttttcc
    10981 ggaaaggcgg ccatccggtg ttatctccct gattcagaat gccaaaaatt tcagataaag
    11041 catcaggaat gacacggcaa gcgtcaatag tgttacgatg atgcccgtct tgaaaaattc
    11101 cacgaacctg atttttattc ctttacttcc tgccgattca atgacgatga ggtttgccat
    11161 cgcccctatg atcgtagcgt ttcctgccag cgtggaggcc gaagccaggg ccagccatag
    11221 cagttcggaa ccggcggttt tcatcagcgg aagcatcacg acggtaaacg gaacgttgct
    11281 gaccacctgc gaaagcacga gactgatcag gtgaataccc gccaatcctt tggcatcagc
    11341 tttcagtgcg acaccatgaa tcagggaatc catgatccct actttttcga tgcctttcac
    11401 cacgataaac agcgaggcga aaaacaacag caacacccag tcgaccttgc ggataaccag
    11461 cgatggcttt atctttccga atatcaaaat cagcgagcct cccaacaggg caatcatcgg
    11521 caccgataat ccgatgcggt ggctcatgaa aaataaaacc acgaccagca ggaaaacagg
    11581 cactgaagtg ttcatcgacc ggaaattgta ctcaaagaga tgattttcgt ttccggctgt
    11641 attatcttcg ggaatcgggt taaaacgcag atctttccgg taaaaccagc ggatgaccgc
    11701 aatcgtgatg aacagcccga tgaatgaaac ggggagcagg tgccacagga aggttccgta
    11761 attgatcccc gatgatatcc cgatcagcat attttgcgga ttcccggtaa tggtcatggc
    11821 ggaaccgatg ttggaagcca tgatttcggc gatcaggtaa ggcatggggt tcgtccgcaa
    11881 cgaacggcat atcaggataa taaccggggt gaaaagcatg acgacggcgt cgttcaccag
    11941 gaaagcgctg gtgatgcccg tcacaagggt gatgatcacc agcaacctgt cgggggtttt
    12001 ggccagcgac atggttttag aagcaatcaa cgaaaagaaa ccgtcgagtt cgagcgtggc
    12061 aataatgatc atcatgccca gcaacagggc aatcgtattg aaatcaatgg cttgtatggc
    12121 ttcttcgaaa cttaatacgc cgaacaaaat catagccacc gcgccaaaaa aggccgccga
    12181 cggacggtcg atattcattt tgggcaaccg ggtaaagatg atccccgtat aggtgaccag
    12241 gaagataact aacgcagtgt aatacaggtt cataatgatc ggttataccg gtaacagaaa
    12301 aagcaggcga tccggttata actaaaccac tatggattaa aaatccatag gttggtaata
    12361 aggtaaaaaa tga
//
LOCUS       DAOPYV010000081        12191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_5838_length_12191_cov_11.131345, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000081 DAOPYV010000000
VERSION     DAOPYV010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12191
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5838_length_12191_cov_11.131345"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="PKI35_09900"
     CDS             <1..177
                     /locus_tag="PKI35_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019035558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNX44738.1"
                     /translation="MPSISSIDAVLNYSHHNYMYFCAREDFSGYHTFAVTQQQHEVNA
                     ARYQQALDKQGILK"
     gene            222..1124
                     /locus_tag="PKI35_09905"
     CDS             222..1124
                     /locus_tag="PKI35_09905"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HNX44739.1"
                     /translation="MALVFLLVVMKPGYCLAEKDTVSSSGSINKKRLTGVLTGGTLVY
                     ATGMIALYGSWYRNYEQSPFHFFDDSREWLQVDKTGHLTTSYYVGYLGYQSFRYTGMD
                     EKRSALLGGLTSWLSMATIEVFDGFSEQWGASVTDLCANTLGSALFTSQQIVWHEQRI
                     LVKWSYHPTKFPDYRPDLLGDNFAGKMLKDYNGHTYWLSVNLKSFLDKNSGFPAWLNF
                     AFGYGAEGMTGAAVNSSVYNGKAIPHFERQRQYYFGPDIDLTRIRTRSKVLNALCKSV
                     GFIRLPLPAVEFSKSGICFHPLYF"
     gene            1158..1568
                     /locus_tag="PKI35_09910"
     CDS             1158..1568
                     /locus_tag="PKI35_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX44740.1"
                     /translation="MKNIHILFIISALFILVSCKSARERSLNYIKEGNALERISEFAK
                     AQEKYQQAIDAEPGNASAWFFMGNVKRNQGMTKESITYYNKAIELDSTFADAYANRGD
                     AKFSLKDRDGSCKDYLKAEELGKENMDEKTKWCR"
     gene            complement(1599..2540)
                     /locus_tag="PKI35_09915"
     CDS             complement(1599..2540)
                     /locus_tag="PKI35_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNX44741.1"
                     /translation="MKLSLPGVCLIIITTVFAATCVAQTPSMAQTPDNRTLEDRLNLQ
                     DSVILNLNGSLSAMQNRMDSITLVNQQAENQKSTMLSFLRKPFFEEKFSALDVLLAIF
                     AGFAGLVALLVSIFVPLSFLRENRRLRKEMRTLFSEIQTLKLYAQSRENRNIAVPGQE
                     LQQEKNPVGDEPPAETATEQKPGVSPLKKDISTAEFLARLELCIIANREDEFNRVVAE
                     VVQYTASKDTTALLRYLHRIAACVFRGRMPAQAPVMAEVKQYLWACESPVFYQTDKIV
                     SWARSSTRLSDAQRAYIFSISTELEKMLAEHNREYND"
     gene            complement(2573..3661)
                     /gene="ychF"
                     /locus_tag="PKI35_09920"
     CDS             complement(2573..3661)
                     /gene="ychF"
                     /locus_tag="PKI35_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNX44742.1"
                     /translation="MGLKCGIIGLSNTGKTTLFNCVANTKVQNQPGIQKANIGQIDVP
                     DERLFEIDKLIHSARIVHATVDLVDIPGLSKGSGQADSGASRFLADIQQTDALIHVIR
                     CFDSDTVPHIEGSVNPVRDKEIVDLELCIRDLNLIERKIQRTDKLLKVGDKEARKIME
                     VLQVYKKHLEDFKPARTAPVQDEDRRYISELSLITDKPVIYVCNVDDGSAVNGNSYVE
                     EFRNSLRDENAEVLWLAAELEAEIAELDNSDDRKAFLEDAGLSQPGVNKVVQAAYRLL
                     NLQSFFTAGPKEVRAWTIHKGMTAPQAAGVIHSDLERGFIRAEVMKYSDFILYKSEHA
                     CRDAGKLSIEGKNYVIGDGDILNIRFNV"
     gene            complement(3890..4279)
                     /locus_tag="PKI35_09925"
     CDS             complement(3890..4279)
                     /locus_tag="PKI35_09925"
                     /inference="COORDINATES: protein motif:HMM:NF039669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6146 family protein"
                     /protein_id="HNX44743.1"
                     /translation="MKIHLVIAAILLIPAACSAQQKTTGNTGLKEQNSTDSTEYEITI
                     MDSQFDYWYQTHYTRAMDRDNQTYRSYNRIGVMNWNDYFILGKHRNIIDEHLNYDYNI
                     DYGIEVNRKLFWYFKYIQEEYGIRLLY"
     gene            complement(4376..6703)
                     /gene="pbpC"
                     /locus_tag="PKI35_09930"
     CDS             complement(4376..6703)
                     /gene="pbpC"
                     /locus_tag="PKI35_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNX44744.1"
                     /translation="MRNLYRRYLSAVPRWARLTLRAILAAFILFVIIDAIFPVKTSVE
                     YSCQILANDSTLLHSFLTSDDKWRMETSPEDVNPLFIRTLIMKEDRWFRWHPGINPVS
                     VARALWQNISKGERFSGASTITMQVVRLLEPRKRTYFNKIIEMFRALQLEFHLSKDEI
                     LKLYINLLPYGGNIEGVRAASFLYFGQEPQALSQAQIVTLAVIPNNPYNLRLGIHNER
                     IVGERNKWLRRLSGKGLFKQGELSDALSEPLSAFRREAPKDLPHLSIRLHNAAGYGTT
                     VYTYIDRQLQSNVEEILRTEINPCRSLGITNAAVVVVDNRSHGILAYAGSAGFSETMF
                     SGQVDGVAALRSPGSALKPFLYALAIDRGLITAKTMLTDIPMNFNGYRPQNFDQTFRG
                     LLTAGDALALSLNVPAVELANRMGADFFNSELARAGFHWIEKKRKSLGLSVVLGGCGV
                     SLLEMSRLFSSFANQGINFPLRFSKSEISGHVDTLVSPEAAWMVTEILTGLKRPDLPT
                     NFDNAVHLPHIAWKTGTSYGRRDAWSIGYNPEYTVGVWVGNFNGEGVPELAGSDFAAP
                     VLFKVFTVLTYNKSSTWFRKPAKIDFRLVCAESGLPPSDFCTNMVMEDYIPGVSPNRK
                     CTHLRTVFTDEQGAVSYCRDCLPPTGYRTALYPDLPPTLIAFYEQEHIPYLKIPPHNP
                     ACTKVFRQEGPLITSLTDGKEYIIYDGSKQQLLLTCAAGADVNKVYWYINDKFYKQAA
                     PSEKVFFTPEPGKIKISCSDDKARNSDIRITVSEF"
     gene            complement(6783..7835)
                     /gene="selD"
                     /locus_tag="PKI35_09935"
     CDS             complement(6783..7835)
                     /gene="selD"
                     /locus_tag="PKI35_09935"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNX44745.1"
                     /translation="MPSEEPVKLTHFSHGMGCGCKMRPQYLEKILKSLPVPSDEKILV
                     GTGSSDDAAVYRINDEQAVVQTIDFFTPIVDNPYDFGAIAATNAISDIYAMGATPLFA
                     LNVVAFPCKTLSPDILEQILKGASDKAAEAGIAVIGGHSIDDNEPKFGMTVTGIVHPA
                     RILANNHALPGDVIILTKPLGTGIISTALRKGIADSSDLAVAVKWMTTLNRQASETIR
                     EFDVHACTDVTGFGLAGHLTEITRGSGVEAEVHFSDMPFMPNVRKYAGLDAIPGGTRG
                     NYDFYSAYINWDEELSLVERYMICDAQTSGGLLFTVPENQSEDLLTKLHGNYCPEARI
                     VGKINGIGNGSVTISV"
     gene            complement(7837..8871)
                     /gene="mnmH"
                     /locus_tag="PKI35_09940"
     CDS             complement(7837..8871)
                     /gene="mnmH"
                     /locus_tag="PKI35_09940"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNX44746.1"
                     /translation="MPYLLNPDQFLKQAAKIPVIDVRSPAEFRQGHIHGAFNVPLFND
                     DERARVGTTYVKTGCVEAKNLGYTIVEPKFDSFLKAASEIAPAKQLLIHCWRGGMRSE
                     SLAGFYTENGYKAGLLRGGYKAYRHYIRQEFSRPCNTILLGGMTGCGKTEILGALASM
                     NRQVIDLEALACHKGSSFGHLGLEGQPTTEQFENDLYALWDAIDDKEPVWIEHESNSI
                     GAVFLPDPFYAKMNGSNMLRINLPFKIRVRRIVAEYASFPAAQLEDALLRIRLRMGTL
                     AFREAVSALGIKDFEKVAEITLEYYDKLYALGLERHPVKNVVDIQLQGDDPEINAAIV
                     LETAQTNKLL"
     gene            complement(9033..>12191)
                     /locus_tag="PKI35_09945"
     CDS             complement(9033..>12191)
                     /locus_tag="PKI35_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNX44747.1"
                     /translation="MKNFEIYKSSAGSGKTYTLVLRYLSLALREKGYFRYILAITFTN
                     KAANEMKQRVVNGLIHLSDPLKFAQTSTVKFMLPDLVKETSLEASEITRRAGEELRFL
                     LHDYDNFTISTIDSFVTKIIRTFAHDLYLPVDFAIETDQERILTEAIDRLLSRAGLDD
                     EITRVLVEFTESKAEDEKDWRIENDLKEIAKELFREESYGPVKQLSNVPLEKYHELTV
                     SIKHFTKAFEDELAGYAASALTLMKENGVQPEWLYQKNNGIAGFFSKTARKEFTLEEN
                     KYVRKTIEEDWWYSKGTAPDIQRAVDAISSRLKEQYLLIFNRLQQQGPTYFLLKMVGN
                     VLYQVAVLGEIEKELEGVKRDKNLLFISEFNKLITDIIIREPVPFIYERIGERFRHYL
                     IDEFQDTSELQWKNLLPLIANSLSGGNINLVVGDGKQAIYRFRNGQVDQFRVLPDLPG
                     HFEKEVFGEAGNALNENGATTILERNFRSKPAVVNFNNRFFRFVADRMPGRIKEIYKG
                     LEQKFNPSEDQGFVSVEWYDKQNENFINANLNRIEELVWYLTDEAGYRKQDIMILAAK
                     NENGSRIAQHLTSKGINVISSESLLLKASPEVNLLVSAFRWLVEPGNTIHAIAVCNFL
                     FELQKIGGERDAAFSRCAQTGIVKFLQAEGFAFSPSKLLSLTVYDQTEELIRIFGLNA
                     GETNPYMQFFLDFVNKLAGTDTLQPADVVKAWDEKCDKLSIVVPEGADAVRIMTIHKA
                     KGLEFPVVIYSFAASDAKYGRKKLWVKTENKELPGLPAALLTTGESLATVGYEDAFLE
                     EREKTWLDTVNLMYVAMTRPADRLYILTKDVEAKDEDNSVQGLINRFIASLGEGDCFR
                     ESNRCGFGSDNPIITAEKDNSKVRPITGNWISVPWQDRIRISGRAPSSWDACTAESRE
                     AWGNIVHKALAMTGSIADINQVTSGLVDQGLLGLEKEAELQQKLYSVVTHPDLKIFFG
                     AFTGFKTEAEIILPGGNVLRPDRVITADNEIVIIDYKTGMRSPHHAGQLHEYAEALRE
                     MGHDNVKSLLVYIDDGVVVEEV"
BASE COUNT         3046 a         2957 c         2824 g         3364 t
ORIGIN      
        1 atgccttcca tttcatccat cgatgcggtc ctgaactatt cccatcataa ttacatgtat
       61 ttctgcgcca gggaagattt ttcgggatac catactttcg ccgttaccca gcagcagcat
      121 gaagtcaatg ctgcacgtta ccaacaggct cttgataaac aaggcatttt gaaataaacg
      181 ctggcaagat ggcaataagc gggaattttt catttcggtt catggctttg gtattcctgc
      241 ttgttgttat gaaacccgga tattgcctcg ctgaaaagga tacggtttcg tcatccggtt
      301 caattaataa aaaaaggctt actggggtat taaccggcgg cacgctggtg tacgccaccg
      361 gaatgattgc cctgtacgga agctggtaca gaaactacga gcaatccccg tttcattttt
      421 ttgacgatag ccgcgaatgg cttcaggtgg ataagacagg tcatttaaca acctcctatt
      481 atgtgggata cctgggatac caatcgttcc ggtataccgg catggatgaa aagagatctg
      541 cgttgctggg aggattaact tcatggcttt ccatggcaac catcgaagtt tttgatggtt
      601 tttcagaaca atggggcgct tcagtcactg atttgtgtgc aaatacccta gggagcgccc
      661 tttttaccag tcagcaaatt gtgtggcatg agcagcgcat ccttgtgaaa tggtcgtatc
      721 accctaccaa attcccggat taccgtcctg atctgcttgg tgataatttc gcagggaaaa
      781 tgctgaagga ttacaacggc catacgtatt ggttatcggt taacctgaaa tcgtttttag
      841 ataaaaattc aggtttcccc gcctggctta attttgcgtt cggatacggg gctgaaggaa
      901 tgacaggcgc agccgtcaat tcctcggtgt acaacgggaa agcaattccg cattttgaaa
      961 ggcaaagaca gtattatttc ggtcccgata tagaccttac ccggataaga acgcgatcaa
     1021 aagtattaaa tgcgctgtgt aaatccgtcg gatttatccg gttacctttg ccggccgttg
     1081 aattcagtaa aagcgggata tgttttcatc cgctgtattt ctgatatttc ctaattttgt
     1141 caaaaagaac cgtcgccatg aaaaatattc atatcttatt cataataagc gctttgttta
     1201 tacttgtttc atgtaaatct gcccgtgaac gctccctgaa ttatattaag gaaggaaatg
     1261 ccctggaacg catttcggaa tttgccaagg cacaggagaa ataccagcaa gcaattgatg
     1321 ccgaacccgg aaatgccagt gcatggtttt tcatggggaa cgtgaaacgg aaccagggaa
     1381 tgaccaaaga atcaatcact tattataata aggcgatcga acttgacagc acatttgcag
     1441 atgcttatgc caatcgcggc gatgcgaaat tctcgctgaa ggaccgggat ggttcctgta
     1501 aggattacct gaaagctgaa gaacttggca aggagaatat ggatgaaaag accaaatggt
     1561 gcagatagcc tgttgtaccg gtcagcattt acctgttttt agtcattgta ctcgcggtta
     1621 tgttcggcca gcattttctc aagctcggtg gaaatactga aaatatatgc gcgctgggca
     1681 tcggatagcc gcgtagaact gcgcgcccac gaaacaattt tatcagtctg gtaaaaaacg
     1741 ggcgattcac aagcccacag atactgtttt acttcagcca ttacaggcgc ctgggcgggc
     1801 atacgtcccc tgaagacgca ggcggcaatc ctgtgaaggt atctcagcaa tgccgtagta
     1861 tcttttgagg cagtgtactg gactacttcg gccactaccc ggttaaattc gtcttcacgg
     1921 tttgcgatga tacaaagttc cagcctggcc agaaattctg cggtcgaaat atccttcttt
     1981 aacggtgaaa cgcccggttt ttgttccgtg gctgtttcag ccggtggttc gtcaccgacg
     2041 ggatttttct cctgctgcag ttcctgacct ggaacagcaa tattgcggtt ttcccgtgat
     2101 tgagcataaa gctttaaagt ttgaatttcg ctgaataacg ttcgcatttc tttcctcaaa
     2161 cgacggtttt cacgcaggaa cgataacgga acaaaaattg aaaccaggag ggccaccagt
     2221 ccggcgaaac ctgcaaatat tgccagcaaa acatccaaag cagagaattt ctcctcaaaa
     2281 aatggtttcc tgaggaaaga aagcatcgta cttttctggt tttcagcctg ttgattgacc
     2341 agtgttatac tgtccatcct gttttgcatg gccgaaagcg aaccattcag gttcaatatc
     2401 acagaatcct gcagattcag cctgtcttcg agggtgcggt tatccggcgt ctgcgccatg
     2461 gaaggggttt gtgcaacgca ggttgcggcg aaaacagtcg ttataataat caaacatact
     2521 ccgggcaggg atagtttcat gacagaaata tattgtttaa ctcacagtaa acttacacat
     2581 taaaacgaat attcagtata tctccatccc cgatgacata atttttccct tcaatggata
     2641 gttttccggc atcgcggcag gcatgttctg atttgtacag gataaaatcg ctgtatttca
     2701 tcacttcggc acggataaac ccgcgttcca ggtcgctatg gatcaccccg gcagcctgag
     2761 gcgccgtcat acctttgtgg attgtccagg ccctgacttc tttcggtccg gcggtaaaaa
     2821 acgactggag gttgagcagc ctgtaagctg cctgcaccac tttattaacg cctggttgtg
     2881 aaagtccggc atcctccagg aatgccttcc tgtcgtcaga attgtcaagt tccgcaattt
     2941 cggcttcaag ttcggctgcc agccaaagca cttctgcatt ttcatccctg agggaattcc
     3001 tgaattcctc aacatagctg ttcccgttaa cagccgagcc atcatcaacg ttacaaacgt
     3061 atatgaccgg tttatcagtg atgaggctca gttcggagat ataccggcgg tcttcgtcct
     3121 ggacaggggc tgtgcgggcg ggtttgaaat cttcgaggtg ctttttatat acctgcaata
     3181 cttccatgat tttgcgggct tccttatccc ctactttcag gagtttatcg gtacgctgga
     3241 tttttcgttc gataaggttc aggtcgcgga tacataattc aaggtcgacg atttccttgt
     3301 cacggacagg gtttaccgac ccttcgatgt gcgggaccgt atcgctgtcg aagcaccgga
     3361 taacatggat gagcgcatct gtttgctgaa tatcggccag aaaacggctt gccccgctgt
     3421 cggcctgacc ggaacctttg cttaggccgg gaatatctac caggtcaaca gtagcatgga
     3481 caatacgcgc cgaatgaata agcttatcta tctcaaaaag acgttcgtcg ggtacatcaa
     3541 tctgaccaat attggccttt tgaattcccg gctgattttg cactttggta ttggcaacac
     3601 aattaaaaag agtagttttc cctgtatttg acagtcctat aatgccacat tttaatccca
     3661 tattcctgtt tacttaatta tgtagcaaat atagggcaat tggccgcggt ttcaaactaa
     3721 tcgataattt acacagtcat ggattttgtc ccgggaaacc ggcaccggat tactgcaatt
     3781 tatggctgta tctgctccaa accgtaatgg cacttagcca gcttaaaaat caccccctgc
     3841 catcgatgga caagaccgcg ccataacata ttgccggttt tgttcccctt cagtacagaa
     3901 gccggatgcc atattcctcc tgaatgtatt taaagtacca gaacaatttc ctgttcacct
     3961 cgatgccata atcaatgtta taatcatagt tgaggtgttc atcgatgata ttccggtgct
     4021 tcccgaggat gaagtaatcg ttccagttca taacgcctat tctgttatag gaacggtatg
     4081 tctggttatc gcggtccatg gcgcgggtgt aatgggtttg gtaccaatag tcgaactgag
     4141 aatccataat agtgatttcg tattcagtgc tgtcggtact gttttgctct ttcagtccgg
     4201 tgttcccggt agttttttgc tgcgcggagc atgctgcggg aatcagcagt atggctgcaa
     4261 ttaccaaatg gattttcatg gcttttttcg tttcaaacag ttgaaatgcc gcaatttatg
     4321 taccatttga atcaaatata cacgataatc gcatcattgt tacgatatct tctggttaaa
     4381 attccgaaac cgtgatccgg atatcgctgt ttcttgcttt gtcgtcgctg catgatattt
     4441 ttatctttcc cggttcaggc gtaaaaaaca ccttttcgga tggggcagcc tgcttataga
     4501 atttatcgtt gatataccag taaaccttgt ttacatcagc cccggcagcg catgtaagca
     4561 acagttgctg ttttgagccg tcataaataa tgtattcctt gccgtcggtg agggaagtta
     4621 tcaaagggcc ttcctgcctg aatacctttg tgcaggcggg gttatggggc ggaattttca
     4681 ggtatggaat gtgttcctgt tcataaaaag cgataagcgt cggaggcaag tcaggatata
     4741 aagccgtgcg atagccggtt ggcggcaggc agtcgcggca ataagaaacg gcaccctgct
     4801 catcggtaaa aacggttctg aggtgggtgc atttccggtt tggtgaaacg cccgggatat
     4861 aatcttccat caccatgttg gtacagaaat cggatggcgg caaaccggat tcggcgcaaa
     4921 caagacggaa atctatcttt gccggtttcc tgaaccatgt tgatgatttg ttatacgtca
     4981 acactgtaaa cactttaaag aggacaggcg cggcaaaatc gctgcctgcc agttcaggga
     5041 cgccttcccc gttaaaattt cccacccata cgcctaccgt atattcaggg ttgtagccta
     5101 tgctccaggc atcgcggcgg ccgtatgaag tccctgtttt ccaggctata tgcggcaggt
     5161 gcactgcatt gtcgaaattg gtgggaagat cgggacgttt cagtccggtc aggatttcgg
     5221 tcaccatcca tgccgcttca ggactgacaa gggtatcgac atggccggaa atttccgatt
     5281 tgctgaaacg taacgggaaa tttattccct gattggcaaa ggaagaaaac aaccgtgaca
     5341 tttcgagcag gctaacgcca caacctccca aaactacgga taaacccagt gatttccgtt
     5401 tcttttctat ccagtgaaag cctgcccgtg caagttcgct gttgaagaaa tccgctccca
     5461 tccggttggc cagttcaacg gcaggaacat ttaacgagag cgccagcgca tccccggctg
     5521 tcagcaatcc ccggaaagtc tggtcgaaat tctgcggcct gtaaccatta aagttcatcg
     5581 ggatatcggt gagcatggtt ttcgctgtaa taagcccccg gtcgattgcc agcgcataga
     5641 ggaacggttt cagcgcgctt cccggcgaac gcagggctgc aacaccatca acctggccgc
     5701 tgaacatagt ttcgctgaag ccggccgaac cggcataggc caggatgcca tgcgacctgt
     5761 tatcgacaac cacaacggcg gcattggtaa tacccagtga acgacaggga ttgatttccg
     5821 ttcttaaaat ctcttcaacg ttcgactgca actgcctgtc gatataggta taaacggttg
     5881 ttccatagcc tgctgcatta tgcaacctga tggaaaggtg cggcaaatct ttgggcgctt
     5941 cccttcggaa tgccgataat ggttccgaaa gcgcatccga cagttcaccc tgtttaaaca
     6001 gccctttccc tgaaagcctg cgaagccatt tatttctttc accgacaatc ctttcgttat
     6061 ggatgcccag cctgagatta tagggattgt tagggataac ggcgagcgtc actatctgtg
     6121 cctgactaag cgcctgcggc tcctgtccga aataaaggaa agatgccgcg cggacgcctt
     6181 caatgtttcc gccgtaaggc aacaggttga tgtagagctt gagaatttcg tccttcgaca
     6241 ggtggaattc aagttgcaac gccctgaaca tctcgatgat cttattgaaa taggttcttt
     6301 ttcgcggttc cagcaaacgc acaacctgca tggtgatcgt gcttgcgccc gaaaatcgtt
     6361 ctcctttcga gatgttttgc cacaaggccc ttgccaccga aaccgggtta ataccgggat
     6421 gccacctgaa ccagcggtct tctttcatta tcagcgtcct gatgaacagc gggtttacat
     6481 cctcaggtga agtttccatc cgccatttat catcactcgt aaggaaagaa tgaaggagcg
     6541 tactgtcgtt ggccagaatc tgacaggaat attcaaccga ggttttgacc gggaagattg
     6601 catcaataat gacaaacaaa ataaaagcag ccaggatcgc ccgcaatgtt aaccgcgccc
     6661 atcggggaac ggctgataaa taccgccggt acaaattcct catcccggtc ccttttttta
     6721 aaccatcggc caccttttct caatgatcag gttataaagt atttttcaat tctttctgcc
     6781 ggttaaaccg aaatagtcac cgaaccattg ccaatgccgt tgatcttgcc gacgatacga
     6841 gcttcaggac agtaattgcc atgcaatttg gtcagcaagt cttccgactg attttcaggc
     6901 acggtaaata ataatccacc ggaggtttgg gcatcacaga tcatataccg ttcaaccagg
     6961 gagagttctt catcccagtt tatatatgca gaataaaaat cgtaattgcc gcgggttccg
     7021 ccggggatgg catccaaccc ggcatatttc cggacattgg gcataaaagg catatccgaa
     7081 aaatgcactt cagcttctac cccgcttccg cgggttattt cggtcaggtg gccggccagc
     7141 ccgaagcctg taacgtcggt gcaggcgtga acatcaaatt cccgtatggt ttccgaagcc
     7201 tgcctgttta gtgttgtcat ccattttacg gctacagcca gatcgctact gtcggcgata
     7261 ccttttcgca gcgcagtaga gattattcca gtgcctaatg gttttgtaag gataattaca
     7321 tctcccggca gcgcatgatt attggctaaa atcctggccg gatgtacaat cccggtcacc
     7381 gtcattccga atttcggctc gttatcgtcg atactgtgtc cgccgataac cgcaattccg
     7441 gcttcagccg ctttatcgga agctcctttc agaatctgct ccagaatatc cggagatagt
     7501 gtcttacagg gaaaggccac cacattcagg gcaaaaagag gcgtggcgcc catggcgtaa
     7561 atgtcgctga tggcgttggt tgcagcaatg gctccaaaat cgtaaggatt gtcgacaatt
     7621 ggagtgaaaa aatcaattgt ctgaaccaca gcctgttcgt catttatacg gtatacggca
     7681 gcatcgtcgc tgctcccggt tccaaccagg attttctcgt ccgaaggcac aggcagcgac
     7741 ttaagtattt tttcgaggta ttgggggcgc attttacacc cgcaacccat tccgtgcgag
     7801 aaatgagtga gtttcacagg ttcttctgaa ggcatgttat aacaatttat tagtttgtgc
     7861 agtttctaaa acaatggcgg cattaatttc aggatcgtca ccctgaagct gaatatcgac
     7921 tacgtttttc accggatgcc tttccagccc caaagcgtaa agtttatcat aatattcaag
     7981 tgtaatttca gccactttct caaaatcttt gatcccgagg gccgatacag cttccctgaa
     8041 agctaatgtc cccattctca gcctgatgcg cagcaaggca tcttcaagct gtgcggcagg
     8101 aaatgatgca tattcagcaa caattcttct gacgcgtatt ttaaatggca ggttgatgcg
     8161 taacatgttg ctgccgttca tcttggcata aaaagggtcc ggtaaaaaaa ctgcgccgat
     8221 gctgttactt tcatgttcta tccataccgg ttctttatcg tcaattgcat cccataaggc
     8281 atacaagtcg ttttcaaatt gttcggtggt cggctggcct tccaaaccga ggtgcccgaa
     8341 tgatgaacct ttatgacagg caagcgcttc gaggtctatc acctggcggt tcatggaagc
     8401 aagggcgcct aaaatttctg ttttcccgca tccggtcatg cctcccaaca ggatagtatt
     8461 gcagggcctg ctaaattcct gcctgatata atgccggtaa gctttgtatc ctccccgcag
     8521 taacccggct ttatatccgt tttcggtata aaaaccggct aaactttcgc ttcgcatacc
     8581 gccgcgccag cagtggatca acagttgctt cgcgggggca atttcagaag ctgctttgag
     8641 gaaagaatca aatttaggct caacgattgt atatcccaga ttttttgctt ccacacaacc
     8701 tgttttcaca taagttgtcc caaccctggc ccgctcgtca tcattaaaaa ggggaacatt
     8761 gaacgccccg tgaatgtgtc cctgcctgaa ttcagccggg gaacgcacat caattacagg
     8821 gattttggct gcctgtttca ggaattggtc aggattcagg aggtaaggca ttatcagagt
     8881 agtttaaatt tacagaagtc cggagtttgc agtcagaagc aataaaaaaa acagatccgc
     8941 ttttgtattc gcgttgcatc cgtattagaa gccggcaggc agcagatcaa acgaataacc
     9001 acggatcgta ctttgttcag gatgcgatgg gtttaaactt cttccacaac tactccgtca
     9061 tcaatgtaaa cgagcaggct tttcacgttg tcatgcccca tttcccgaag ggcttcagcg
     9121 tattcgtgca gttgcccggc atgatggggt gaacgcattc ccgttttata atcaatgatg
     9181 acaatttcgt tatcagcagt tatcaccctg tcgggacgca gcacatttcc tcccggcaaa
     9241 attatctcag cttccgtttt aaaaccagta aaagctccaa aaaagatttt caaatccgga
     9301 tgggtaacca ccgaatataa tttctgctgt aattcagctt ctttttccaa accgagcaaa
     9361 ccctgatcca ccaatccgga tgttacctgg ttaatatcgg caatgctccc ggtcatggca
     9421 agggccttat ggacaatgtt tccccaggct tcacggcttt cggcggtaca ggcatcccag
     9481 gaggacggcg cccggcccga aattctgatc cggtcctgcc agggtaccga aatccagttt
     9541 ccggttatcg gccgaacctt gctgttatcc ttctctgccg ttataatcgg attgtcactc
     9601 ccaaacccac accggtttga ttctctgaaa cagtcaccct cgccgagtga agctataaac
     9661 ctgttgatca gtccctgaac tgaattatct tcatcttttg cttcaacatc tttggtcagg
     9721 atatacaagc ggtcggcggg gcgggtcatc gccacataca tcaggttaac cgtgtcgagc
     9781 catgtttttt ctctttcttc aaggaatgca tcttcataac cgaccgttgc aaggctttcg
     9841 ccggttgtca gcaatgctgc cggtaaacct ggaagttcct tattttctgt ttttacccat
     9901 aatttctttc gtccgtattt tgcatcggag gctgcaaaac tgtaaatcac tactggaaat
     9961 tccaaccctt tggccttgtg tatggtcata atgcgcacgg catcagcgcc ttcggggaca
    10021 actatggaga gcttatcaca tttttcgtcc caggctttca cgacgtcggc tggctgcagg
    10081 gtatcggtac cggccagttt attgacgaaa tcgagaaaaa actgcatata tggattcgtt
    10141 tctccggcat tcagcccgaa aatcctgatc aactcttccg tctggtcgta aacggttaac
    10201 gacaggagtt tggatggcga aaaagcgaaa ccttcggcct gaaggaactt cacgatccct
    10261 gtttgggcac agcggctgaa agcagcatcg cgttctcctc cgatcttttg cagttcgaat
    10321 aaaaaattgc agaccgcgat ggcatgtatc gtatttcccg gttcaaccag ccaccggaaa
    10381 gcggaaacca gcaggtttac ttcaggtgag gctttaagca ataatgattc actggatatt
    10441 acgttaatgc ctttactggt gagatgttgt gcaatccggc tcccgttttc gttttttgcg
    10501 gcaagaatca tgatatcctg cttccggtaa ccggcctcgt cagtcaggta ccagaccagt
    10561 tcttcgatcc ggttaagatt ggcattgata aaattctcat tttgtttatc ataccattcc
    10621 accgaaacaa aaccctgatc ctccgaagga ttgaactttt gttccaatcc cttataaatt
    10681 tcctttatcc ggcccggcat ccgatcggct acgaaacgaa aaaaccggtt attaaagtta
    10741 acgactgctg gttttgagcg gaaattcctc tccaggatcg ttgtggcgcc attctcgttc
    10801 agggcatttc cggcttcgcc aaatacctct ttttcaaaat ggccgggcag atcaggcaga
    10861 accctgaact ggtcaacctg gccgttcctg aaacggtaaa ttgcctgctt accgtcgccg
    10921 accaccaggt ttatatttcc gccggaaagc gaattggcaa tcaacggcag caggtttttc
    10981 cactggagtt cagaagtatc ctggaattca tcgatcaggt aatgccggaa ccgttcgccg
    11041 atgcgttcat aaatgaaggg aaccggctcc cggatgataa tatcggtgat gagcttatta
    11101 aattcggaga taaaaagcag gtttttatct cttttcacac cttcgagttc tttctctatt
    11161 tcgcccagta ctgccacctg atagagaaca ttgcctacca tcttcagcag gaaataagtt
    11221 ggcccctgtt gctgtaagcg gttaaaaatc agcaaatact gttctttcag ccggctgctt
    11281 atcgcatcca cggcgcgttg aatgtcaggg gctgtaccct ttgaatacca ccagtcttct
    11341 tcgatggttt tcctgacata tttattctcc tcaagtgtaa attcctttct ggctgttttg
    11401 ctgaaaaacc ccgctatccc gttatttttc tggtaaagcc attccggctg aacgccgttt
    11461 tccttcatca gggttaacgc cgatgcggca tagccggcca gttcgtcttc gaaggcttta
    11521 gtgaaatgtt tgatggaaac ggtaagttcg tggtatttct caaggggaac gttgctcaac
    11581 tgctttaccg ggccataact ttcctccctg aacagctctt tggcaatctc tttcaaatca
    11641 ttttcgatgc gccagtcctt ttcgtcttcg gctttagatt cggtaaattc aaccaggacc
    11701 cgggtaatct catcatcaag ccctgccctg ctcagcaacc ggtcaatcgc ttcggtcaga
    11761 atcctttcct ggtcggtttc aatggcaaag tcaaccggca ggtaaaggtc gtgggcaaag
    11821 gtgcggatga tttttgtcac aaagctatca atggtgctga tagtgaaatt atcgtaatcg
    11881 tgcagcagga acctcagctc ttcaccagcg cgtcgggtta tttccgatgc ttcaagggaa
    11941 gtttctttaa ccaggtcggg gagcatgaat ttcacggtgc tggtttgggc aaacttcagc
    12001 ggatcggaaa gatggataag gccgtttacc accctttgct tcatctcgtt tgcagctttg
    12061 ttggtaaaag tgatggccag gatatacctg aaatagcctt tttccctgag cgccagcgaa
    12121 aggtacctga gcaccagcgt gtaggttttg ccggaaccgg ccgaagattt atagatttcg
    12181 aaatttttca t
//
LOCUS       DAOPYV010000082        12109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_5905_length_12109_cov_11.784553, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000082 DAOPYV010000000
VERSION     DAOPYV010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12109
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5905_length_12109_cov_11.784553"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..556)
                     /locus_tag="PKI35_09950"
     CDS             complement(38..556)
                     /locus_tag="PKI35_09950"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNX44748.1"
                     /translation="MKNFVRIIAIVFLFAAFVPAQAQKNVKLGHVDFAKVIEQMPGQD
                     TVKRVMENYTKSLREQLQAMQTELETKYNDYVSNQASLSAIIKQTKEKEISDLQDRIQ
                     AFQQQAQQDIQEQQDKLTAPFIEKAKKAINDVAKEGSFTYIFNSVEGFILYLDGGEDV
                     TPQVKKKLGITQ"
     gene            complement(660..1202)
                     /locus_tag="PKI35_09955"
     CDS             complement(660..1202)
                     /locus_tag="PKI35_09955"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNX44749.1"
                     /translation="MKKTILTAMLFCAFSFAALAQKYAYVNTGYILDNIPEYADAQAQ
                     LDELSVQWQKEIEAKFTEIDKLYKSFQNESVLLPDDMKKKREDDIINKEKEAKDLQKK
                     RFGNNGDLYKKRQELIKPIQEKVYTAIEEIATAENYALVFDKTGTTTLIYTNPKYDIS
                     EQVLDKLGYTYKPQETENKK"
     gene            complement(1344..3977)
                     /locus_tag="PKI35_09960"
     CDS             complement(1344..3977)
                     /locus_tag="PKI35_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNX44750.1"
                     /translation="MTLRRPLFLVLTFISLAFCAAAQISIGEQIPEFDYYSPKTYTIG
                     GITVSGVDYLDNSVLISISGLRVGDKIELPGDKTHTAITKLWDQGLFEDIEIKVIRVQ
                     DDQVFLNLSLKERPRMQRFSFKGVRKGEADDLREKVKLVSGDVVTDNLIIRTKNLIRK
                     HYIGKGFWDVDVDIDQVHDTSQPNYVALVINVHKHNKVRIQKININGNEILSDGSLKA
                     SMKKTKEKGSYRVLYAIQEGLGIMVNDAFSGSLKKIPADLRDMTDANVKPRIFKASKL
                     IANDYEEDKINLITKYNQAGYRDATILRDSVYKNAGGNLVVDLDVYEGHKYYFRNISF
                     VGNTKYTASDLNTILGIRKGDIYNQEQLDAAISFNPNGFDLMSLYLDDGYLMAQVNPV
                     EMQAENDSIDLEIRIHEGKQMRINKVNISGNTRTNDNVIIREIYSRPGQLFSRSDVIR
                     SRTSLAQMKYFDPEKIDITTPNPNPIDGTVDVAYKVEETSSDQLELSGGWGYSRLIGT
                     LGVSFNNFSAKNIFTKGAWRPVPSGDGQKLSIRMQSYGKGYLSFSTSFTEPWLGGKKP
                     NAFSVSAYASRYSYGTKGSSSYYLYNITGLSFMLSRRLNWPDNYFTLANSINFQLYRL
                     ENYSRFTQLGIGDGFYQNYSYTIALGRYSTDAAIFARSGSEVSLSIEATPPYSLLFRD
                     KYAGMTFEEKNKLVEFHQWKFAGTFYKQIVGDLVVMARVKAGFLGRYNPDMNYTPFNR
                     YFMGGDGMTGYSSAIDGRQLIGFRGYVNESLTPDAYSSTEPGAIIFNKSTLELRYPLS
                     LNPNSTIFGLVFAEAGNSWDNYKEFNPFDVKRSLGVGVRVFLPMFGLLGLDWGYGFDQ
                     IPGMSSANKGQFHFSINSSID"
     gene            complement(3974..4738)
                     /locus_tag="PKI35_09965"
     CDS             complement(3974..4738)
                     /locus_tag="PKI35_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNX44751.1"
                     /translation="MTNSTDLRALVDMQKLPAHVAVIMDGNGRWARQQGHDRIFGHQH
                     GVEAVRQTAEAAAELGIRFLTLYAFSTENWNRPKAEVDALMQLLVTAIHNEIKTLNDN
                     NIRLRAIGDLKAMNPEVLAQLNDAMDLTSGNNRMDLILALSYSGRWEIENAVRTIAGA
                     VKKGELQPEEIDEKTISAFMNAPDVPDPELLIRTSGECRVSNYYLWQLAYTEFYFTNK
                     LWPDFRKSDLYEAVIDFQKRERRFGQTSEQLTNCKE"
     gene            complement(4822..5649)
                     /locus_tag="PKI35_09970"
     CDS             complement(4822..5649)
                     /locus_tag="PKI35_09970"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNX44752.1"
                     /translation="MLKKLILPAFILLFVQELMAQQTGEFGPFAGVSYYVGELNPGMP
                     FRMSKFAFGGLYRQNFTDRLAARIHFLTGSVTGDDAASGIRPDRNLNFTSPITEIGGQ
                     FEVNFFEYYIGSRQHRWSPYLFGGGAFFMFKPYTTLDGSKVELQPLGTEGQGSSRYPD
                     RKAYNLFSFSVPFGMGVKFSVNKYFGLAAEWGMRRTFTDYIDDISKTYYLDLEGADAS
                     SVSREEYLSDPTLSHNTGMQRGNERNSDWYSFAGISATVKIRMLNRERCLDHQRDGY"
     gene            complement(5837..6715)
                     /locus_tag="PKI35_09975"
     CDS             complement(5837..6715)
                     /locus_tag="PKI35_09975"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNX44753.1"
                     /translation="MNIAIYGKTFSPEHLDYFKLLVGKLEEYGCRLKVWKPFYDFISD
                     CKALTPTMQPFSTPAEIHGNTEFLFSVGGDGTLLGSIALVRDSGIPIAGINLGKMGFL
                     SSIPRTEIVAALTDIFQGKYRLENRSLIKLKNPEGLFGDINFGLNELSINKKDSASMV
                     VIRVSVNGCLLHSYWADGLIVATPTGSTAYSMSCNGPIIAPDSQNFVITPIAPHNLTI
                     RPIVIPDNSTISLRIESRDRQALVCLDSRSADIDPDTEIIVCKADFDVKLVQRINDNF
                     FSTLRTKLNWGVDIRN"
     gene            complement(6839..8269)
                     /locus_tag="PKI35_09980"
     CDS             complement(6839..8269)
                     /locus_tag="PKI35_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44754.1"
                     /translation="MKGKHTRIRFSRWWGVLFVLLSVNVPVAGQERTPVIIGHIRTEK
                     NSVTDISVILRELPFREGDTIGADEFDKFVGKSVENLLNTSLFNFVTADTSFVRSEEY
                     RVATLTYKFVERWYVWPAPILEISDRNINAWYQKRDYTRFNYGAHLVWNNVTGRMENL
                     DLMLRFGKNQHCSLLYDIPYIDRHKQIGVGLEAGLIREHEVGYITEDDKLLYAFNKKS
                     MMTGWYTALHILYRHSIYFNHELDIGYQRYSYSDTLLDLNPGYLATGEKHCWYPSVYY
                     KFKADFRDAKYYPLTGWYFDAEIFQAGFDAGDDQSVTSGWIKFTGRIYRKLANRWYAG
                     AGMVTRISTFPDNSYFMNTALGYNRDFVRGYEYYVIDGTHFGVFKSSVKFALIPERAM
                     QIGIIPTEKFSLVHYAAYLTWFGDAGYVCKNGPAGQNNQLPGTLLLGSGLGLDLVTYY
                     DKVMRIEFTLNRKGEKGIYLHFIAGL"
     gene            complement(8306..8968)
                     /locus_tag="PKI35_09985"
     CDS             complement(8306..8968)
                     /locus_tag="PKI35_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNX44755.1"
                     /translation="MIAKDLFTDAVIPLKTSDTGLTALHWMDEFKVEHMPIVNDSEFL
                     GLISEDDIFAFNSFEEPLGNHPLSHQNAYVNQSQHIYDVIRMFSDLKLTLLPVVDDKR
                     KYLGVITLAPLVEKMAAIAAINDPGGIIVLELTANDYSISEIAQIIESNDARVLSLYV
                     TSHSDSTKLELTIKVNRQDIQPILQTFQRYDYLIKASFFESDYYDSLRDRYDQLMNFL
                     NI"
     gene            9106..9822
                     /locus_tag="PKI35_09990"
     CDS             9106..9822
                     /locus_tag="PKI35_09990"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNX44756.1"
                     /translation="MTKLSVNINKIATIRNARGGNMPDVLKAAIDCELFGAQGITVHP
                     RPDERHIRYSDVYEIRPLIRTEFNIEGNPQPRFINLVLDVKPHQVTLVPDAHDAITSN
                     AGWNTIKHKDFLRDVIAAFKKEGIRTSIFVDPDVAMVKNAAETGTDRIELYTEAYARN
                     YPVDRAEALAPYLEAAKAACEAGLGINAGHDLDLNNLWYIARSLPGLQEVSIGHALIS
                     EALYLGLEKTIGLYLQQLKE"
     gene            9851..10435
                     /locus_tag="PKI35_09995"
     CDS             9851..10435
                     /locus_tag="PKI35_09995"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HNX44757.1"
                     /translation="MKYPATIILILLLQAFVSAQGVVDASKLSERENIMYLSGQDTPF
                     SGTAVVWLSPGQKQVESQYLDGRLNGVETTWDNQGNKFTEVTFRNGAFNGPYKQWYPN
                     GQLEIDKIYRDGMMNGPSVHYYDNGQKENEGVYLDCRETGTWTFYYRNGQKLKEGEFV
                     NGFEEGVWKYYQENGTLSKEVIYSDGNVVSEKSF"
     gene            10450..11295
                     /locus_tag="PKI35_10000"
     CDS             10450..11295
                     /locus_tag="PKI35_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44758.1"
                     /translation="MKTMLRIVLAVCCFSGFNVWGQKAEISKAVVKKWETPARLKTPE
                     SVLYDGKDVIYVANINGQPAAKDGNGFISKISLDGRILKSEWITGLDAPKGMGLYSGK
                     LYVTNITEVVEISIAGSKITKRYPAEGSVFLNDISISPSGAVYVSDSRKGTIYRLMNG
                     KLEKWMEGLDGVNGLYCTGKDMLLAGTGNSIVQINTAGKQKSTLVSGTGSIDGLIYCG
                     KNTYLFSDWEGSVYRVVMGKAKELLLNTSKAGINAADIDYIPSKRLLLVPTFRDNRVM
                     AYELK"
     gene            11734..>12109
                     /locus_tag="PKI35_10005"
     CDS             11734..>12109
                     /locus_tag="PKI35_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4372 domain-containing protein"
                     /protein_id="HNX44759.1"
                     /translation="MMDFAGNSLSMNQGKNIFAQLTGFLPRRVFDRIVKEFNGNRYIK
                     TFTCWNQMLCMVFGQLTARDSMRDSILSLEAHQAEYYHLGFGSKVSRRNLGTANEKRS
                     CKIFDEFACVLMVQARRSSYRSV"
BASE COUNT         3170 a         2824 c         2814 g         3301 t
ORIGIN      
        1 ccattggggc agcctctttt aatattatcg ggcatagtta ctgagtaatg ccgagtttct
       61 tttttacctg cggggttaca tcttcgccac cgtcgagata aaggataaat ccttccacac
      121 tgttgaagat ataggtgaag ctgccttctt tggctacatc gttgatagct tttttggctt
      181 tttcgatgaa aggagccgtc agtttatcct gttgttcctg gatatcctgt tgggcttgtt
      241 gctggaaagc ctggatgcgg tcctgaaggt ccgaaatttc cttttccttt gtttgcttga
      301 tgatagccga caacgatgcc tggttgctta catagtcgtt atattttgtt tcgagctcgg
      361 tctgcatggc ctgtaactgt tcccgcagcg atttggtata attttccatc actcttttca
      421 cggtatcctg accaggcatc tgctcaataa ccttagcgaa atccacgtgt cccagtttta
      481 cgtttttctg agcctgggcg ggcacaaagg ctgcaaaaag gaatactatt gcaattattc
      541 taacgaagtt cttcattttt tatgtttatt gaatattaag gtgttatgat ttatacttcg
      601 gagggcggca aagttaaaca atttcctcta attccaaaat cggcaacacc ttgccgcgat
      661 tactttttat tttcggtttc ctggggtttg taagtgtatc cgagtttatc aagcacctgt
      721 tcgctgatat catacttcgg gttggtataa atcagggttg tagttccggt tttatcgaag
      781 accagcgcat aattttcggc cgtagcaatt tcttcgatgg cggtatatac tttctcctgg
      841 ataggtttaa taagttcctg gcgtttcttg tataaatcgc cgttgttgcc aaaacgcttt
      901 ttctgaaggt ctttggcttc tttctcttta ttgatgatat cgtcttcccg ctttttcttc
      961 atatcgtcgg gcaacagcac cgattcattc tgaaaagact tatatagttt atcgatttcg
     1021 gtaaacttgg cctcaatttc tttttgccat tgaaccgaca attcatcaag ctgagcctgg
     1081 gcatcggcat attcgggaat gttatcgaga atgtagccgg tgttgacata tgcatatttc
     1141 tgggctaaag ccgcgaaact gaacgcgcag aacagcatag cggttaaaat cgtttttttc
     1201 atggtcatgc ctccggggct gttgggttag ggtgataaaa gatattttct ttactcaaaa
     1261 agtctgccaa atgatttacc gtattgatca gattactatc ggggaatcct tgtccttgcc
     1321 gaaaattccg ttttcagaga tttttaatcg atggatgagt tgatggagaa atggaactgc
     1381 cctttgtttg cagatgacat accggggatc tgatcgaagc catagcccca gtcgaggccc
     1441 agcagcccga acatcggcag gaaaaccctc acgcccacgc ccaacgaacg ttttacatca
     1501 aatggattga actccttgta attatcccag gaattcccgg cttcggcaaa aaccagtccg
     1561 aaaatagtgc tgttggggtt gagcgaaagc ggatagcgca attcgagggt gctcttattg
     1621 aaaatgatgg cccctggttc ggtagaacta tatgcatcgg gagtaaggct ttcgttgaca
     1681 tatcccctga aacctatgag ctggcggccg tcgatagccg acgaatatcc tgtcattcca
     1741 tcgccgccca taaagtaacg gttgaaaggg gtgtaattca tatcgggatt gtacctgccc
     1801 agaaatccag ctttgaccct ggccatcacc accaggtctc ccacgatttg tttataaaat
     1861 gtgcctgcga acttccactg gtggaattca accagtttgt ttttttcttc gaaagtcatt
     1921 ccggcgtatt tatccctgaa aagaagcgaa tagggcggag ttgcttcaat ggaaaggctt
     1981 acttcactcc cgctccgggc aaagatggcc gcatcggttg agtaacgtcc cagtgcaatg
     2041 gtgtaactgt aattctgata aaatccatcg cctatgccta actgggtgaa cctggaataa
     2101 ttttcgagac ggtagagctg aaaattaata ctgttggcca gcgtaaaata gttgtcgggc
     2161 cagttcaacc gcctgcttaa catgaacgaa agccccgtga tattataaag gtagtaggac
     2221 gaagagcctt tggtaccgta agaataccgg gaggcgtaag cagaaacgct gaaggcgttc
     2281 ggttttttcc cgcccagcca gggttcggta aaggatgtgc tgaagctcag gtatcctttt
     2341 ccgtacgact gcatgcggat agacaatttc tgtccgtcac ccgaaggaac aggtctccag
     2401 gcccctttgg taaatatatt tttggctgaa aagttgttga atgacacccc cagcgtgcct
     2461 atcagcctgc tgtagcccca gcctcccgac aattcaagct gatccgacga tgtttcttcc
     2521 accttgtagg ctacatcaac ggttccgtca atggggttag gatttggggt tgtgatatcg
     2581 atcttttccg gatcaaaata tttcatttgc gccagcgaag tacggctgcg gatcacatcg
     2641 ctcctgctga acaactgccc tggacgggaa tagatctccc ggataatgac attgtcgttg
     2701 gtgcgcgtat tgcccgaaat gttaaccttg ttgatccgca tttgttttcc ttcgtgaatc
     2761 ctgatttcca aatcaataga gtcgttttcg gcctgcatct caacaggatt tacctgggcc
     2821 atcaggtagc cgtcgtcaag atagaggctc atcagatcga aaccattggg attgaacgaa
     2881 atggccgcat caagctgttc ctggttgtaa atatcgcctt tcctgattcc caggatagta
     2941 ttcagatcgg aagctgtata ttttgtattg cctacaaaac ttatgttcct gaaatagtac
     3001 ttatgccctt cataaacatc aaggtcgaca acgaggttcc cgcctgcgtt tttataaaca
     3061 gaatccctca aaatggtcgc gtcgcgatat cctgcctggt tatatttggt gataaggttg
     3121 attttatcct cttcataatc gtttgcaata agttttgacg ctttgaaaat gcgcggcttt
     3181 acattggcat cggtcatatc gcgaagatct gcagggattt ttttcaggct tccggagaag
     3241 gcgtcattca ccataattcc aagtccttcc tgaatcgcat aaaggaccct gtacgatccc
     3301 ttttccttgg tttttttcat ggaggctttc aggctgccat ccgacaaaat ctcgttgccg
     3361 ttgatattga ttttctgaat ccttactttg ttgtgcttat gaacattaat gaccagtgca
     3421 acataattag gctgtgaagt atcgtgtacc tggtcaatat ccacgtcaac atcccagaag
     3481 cctttgccga tataatgttt cctgatcaga ttttttgtac ggatgatcag gttatcggtg
     3541 accacgtcgc cggaaacgag cttaactttc tcacgcaggt catcggcttc acctttcctg
     3601 acgcctttga agctgaaacg ctgcattcgc ggacgttcct tcagggagag attcaggaaa
     3661 acctgatcgt cctgcaccct gatcactttt atttcaatgt cctcgaagag cccctggtcc
     3721 caaagttttg tgatggctgt gtgggtttta tcaccgggaa gttcaatttt atcgcctacg
     3781 cgtaagccac ttatcgaaat cagaacggaa ttatcaaggt aatcaacccc gctcacggtt
     3841 atccctccga tggtataagt tttcggggaa tagtaatcaa attcagggat ttgttcaccg
     3901 atcgatatct gggcagcagc gcaaaaagcc agcgaaataa aagtcagtac taaaaacagt
     3961 ggtcgcctaa gtgtcattct ttgcaatttg ttaattgttc gctggtttgg ccaaaacgtc
     4021 tttcacgttt ctgaaaatca atgactgctt cgtaaaggtc agattttctg aaatcgggcc
     4081 ataatttatt ggtgaagtaa aattccgtat atgccagttg ccataaatag taattgctaa
     4141 ccctgcattc tccgctggtc cggataagca attcaggatc gggaacatcg ggggcattca
     4201 tgaaggctga aatggtcttt tcatcaattt cttccggctg cagttcacct ttttttacag
     4261 ccccggcaat tgttctgact gcattttcaa tctcccacct tcctgaataa ctgagcgcca
     4321 gaataagatc catccggtta tttcccgaag tgagatccat agcatcgttt aattgagcca
     4381 atacttccgg attcatcgct ttcagatcgc caatggcgcg tagccggata ttattatcat
     4441 tgagggtctt aatttcgtta tggatggccg ttaccagcaa ttgcatgagg gcatccactt
     4501 cggctttggg cctgttccag ttttcggttg aaaaggcata gagcgtaagg aacctgatgc
     4561 cgagttcagc agcagcctcc gcagtttggc gaacggcttc cacgccatgc tggtgtccga
     4621 agatacggtc atgcccctgc tgtcgggccc agcggccgtt tccatccatg ataacagcaa
     4681 catgggcagg cagtttctgc atgtcaacaa gggctcgtaa atctgtcgaa tttgtcattt
     4741 ccggtcaaaa ataagtcttt gttgtatttg gcttgccttt cagcgtgtta atttatctta
     4801 aaatgcttag tttcacaaaa atcagtaacc gtcacgctgg tgatccaggc aacgttcccg
     4861 gttcagcatc cggattttta cggttgccga aattcctgca aaactatacc agtccgaatt
     4921 ccgttcatta ccgcgctgca tgcccgtatt atgcgaaagg gtcggatcgc tcagatattc
     4981 ctccctcgaa accgaagaag catcggctcc ttcaaggtca aggtaatagg ttttgctgat
     5041 atcatcgatg tagtcggtga atgttctgcg cataccccat tcggcggcaa ggccaaaata
     5101 tttatttaca ctgaatttca cgcccatccc gaaagggacg gagaatgaaa acaggttata
     5161 ggctttacgg tcaggatacc gggaagagcc ttgtccttcg gtgcccaggg gttgcagttc
     5221 cacttttgaa ccgtcgagcg tggtataagg cttaaacata aagaacgcac ctccgccgaa
     5281 cagataaggg gaccaccggt gctgacggct tccgatataa tattcgaaaa aattcacctc
     5341 aaattgcccc ccgatttcag taatcggcga agtaaaattc aggttacggt cagggcggat
     5401 ccccgaagca gcatcgtcgc cggttaccga tcctgttaag aaatggatcc gggcagccag
     5461 cctgtcggtg aaattctgcc tgtaaaggcc gccaaaagca aattttgaca ttctgaaagg
     5521 catacccgga ttaagttccc cgacatagta ggacaccccg gcaaaagggc caaattcccc
     5581 tgtttgctgc gccataagtt cctggacaaa cagcagaata aacgccggta atattaattt
     5641 tttaagcata tttcaacaag gaagcaaaag tacttaaatt tttgatttgg taaagtgcag
     5701 aaatattcat gcaatccgtt gtaatagcgg tgatttgccg gtatccggaa aggccgccgg
     5761 caaaataccc tgacggcatg tagcctcgat cgggtaatta aaagaagccc ggaaattatt
     5821 gtttacgctg tgacggtcaa ttccggatgt caacgcccca gttgagttta gtgcgaaggg
     5881 tcgaaaaaaa gttgtcattg atccgctgaa ccagtttaac atcaaaatcg gctttgcaaa
     5941 caattatttc ggtatcagga tcaatatcgg ccgaacggct gtcgagacaa acaagcgcct
     6001 gccggtcacg gctttcgatc ctcaggctta tggtactgtt gtcaggaata acgatgggac
     6061 gtatggtcaa attgtgcggg gcgatgggag tgatcacaaa attctgcgaa tcaggggcaa
     6121 taatcggtcc gttgcagctc atcgaatagg ccgttgatcc ggtaggcgtt gctacgatca
     6181 gcccgtcggc ccagtaagaa tggagcagac agccattgac cgataccctt attaccacca
     6241 tggaggcgga atctttctta ttgattgaca gttcgttgag accgaaatta atatcgccga
     6301 acaggccttc cggatttttt aatttgataa gcgaacgatt ttcaagcctg tatttaccct
     6361 gaaagatatc ggtaagggcc gcgacaattt cagttctggg aatgctggag agaaagccca
     6421 ttttccccag gttaatgccg gcaatcggaa ttccggaatc acggaccagc gcaatggagc
     6481 caagaagcgt accatcaccg cccaccgaaa aaaggaattc cgtatttcca tgaatttccg
     6541 caggagttga aaagggctgc atggtaggcg ttaaagcctt gcaatcgctt ataaaatcat
     6601 agaaaggctt ccagactttc aacctgcatc cgtattcctc aagttttccg accagaagtt
     6661 taaaataatc aagatgttcc gggctgaagg tcttcccata tatggctatg ttcactgctt
     6721 ttctttttcc gcaaaattat gaaacagttt tgttattggt tcatcttata ttcatgcccg
     6781 ccgccggaaa gccaaagttt ccggcgcatg aaccgctgcc atttcaaccg ccccggcatc
     6841 agagtcctgc aatgaaatga agatatatac ctttctcccc cttgcggttt aaggtaaact
     6901 ctatgcgcat taccttatca tagtaagtaa ccaggtccaa cccaagtccg gaacccagca
     6961 gcaaagtacc gggcaactgg ttgttctggc cggcaggacc attcttgcat acatacccgg
     7021 catcgccgaa ccaggtcaga taagctgcat aatgaaccag gctgaatttt tccgtcggta
     7081 taattccgat ctgcatggcg cgttccggaa tcagggcaaa cttcactgaa cttttaaaaa
     7141 ccccgaaatg ggtgccgtca atcacataat attcgtagcc cctaacaaaa tcacggttat
     7201 agccaagcgc cgtattcatg aaataggaat tgtccggaaa ggttgaaatc ctggtaacca
     7261 ttccggcccc ggcataccaa cggtttgcca gtttccggta aatcctgccg gtgaatttta
     7321 tccagccgga agttaccgac tggtcatctc cggcgtcgaa accggcctgg aaaatctcgg
     7381 catcgaagta ccatcccgtt aacggatagt attttgcatc tcggaaatca gctttaaact
     7441 tataatatac cgatgggtac cagcaatgct tctcccccgt agccaggtat ccgggattaa
     7501 gatccagcag cgtatcggaa tagctatatc gctggtaacc gatatccagc tcatggttaa
     7561 aatagataga atggcgatat aagatatgca gggctgtgta ccaacccgtc atcatggact
     7621 ttttgttgaa ggcatagagg agtttatcgt cctcggtaat gtagccgact tcatgttccc
     7681 ggatcaggcc ggcctccagt cccaccccga tctgcttatg tcggtcgatg tacggaatat
     7741 catacaacag gctgcaatgc tggtttttgc caaaccgtaa catcaggtca aggttctcca
     7801 tccgcccggt tacattgttc cacacaagat gggcgccata gttaaaccgg gtataatccc
     7861 tcttttggta ccaggcattg atattccggt ccgaaatttc cagaatggga gcaggccata
     7921 cgtaccatcg ctccacaaac ttataggtca gcgttgcaac cctgtactcc tccgaccgga
     7981 caaacgaagt atcggccgtt acaaaattaa aaagcgaggt gttcagcagg ttttcgacgc
     8041 tcttgccgac gaatttatca aattcatcgg caccgattgt atcgccctcc ctgaaaggca
     8101 attcccgaag tataaccgaa atatccgtga cagaattctt ttcagtcctg atatggccga
     8161 taattaccgg cgtcctctcc tgccctgcaa caggaacatt gaccgataac aatacaaata
     8221 atacgcccca ccatcggctg aaccggattc tggtatgctt tcctttcata gtaactgcct
     8281 gtcggggtgt ttcataaatt gaaaatcaga tattcagaaa attcataagc tggtcgtaac
     8341 ggtcgcgcag gctgtcgtag taatcgcttt cgaaaaagga cgctttgatg agataatcgt
     8401 aacgctgaaa tgtctgcaat ataggctgga tatcctgccg gtttaccttg atggtaagtt
     8461 cgagtttggt tgaatcgctg tgggaagtta cgtaaaggct caaaacgcgg gcatcgttcg
     8521 attcgatgat ctgggcaatt tccgatattg aatagtcgtt agccgtaagt tccagcacga
     8581 tgatgccgcc cggatcattg atagctgcaa ttgccgccat tttttcaacc aggggggcca
     8641 gggtgatcac cccgagatat ttcctcttat catccactac cggtaacagg gtaagtttca
     8701 ggtcggaaaa catcctgatg acgtcataaa tatgctggga ctggttaaca tacgcattct
     8761 ggtgcgacaa cggatgattg ccaagaggtt cctcaaagct gttgaaggcg aaaatatcgt
     8821 cttccgaaat gagccccaga aattcactgt cattcacaat gggcatatgt tctaccttaa
     8881 attcatccat ccagtgcaag gccgtcagtc ccgtatcgga tgtcttcagc ggaataacgg
     8941 catcggtaaa caaatctttg gctatcatac ctttaacttt ttatcgttct tgtaatccgg
     9001 aatgaattct ctcattaaaa tgcggcgatg cgtcgcgcat ggtttccaac atttcgggta
     9061 aataaactgt cgcttttgta actttgcggc ataattgtct tactgatgac aaagttaagt
     9121 gtaaatatca ataaaattgc taccatccga aatgcaagag gtggaaacat gcccgatgtg
     9181 ctgaaagccg ctattgattg tgaacttttt ggagcccagg gaattaccgt acatccgcgg
     9241 cccgatgaac gccatatccg gtacagcgat gtttatgaaa tacgtccgct gatccgcacc
     9301 gagtttaaca tagaaggcaa cccccagccc agattcatta atctggtgct tgacgtaaaa
     9361 cctcatcagg ttacgctggt gcccgacgcc cacgatgcca ttacttccaa cgccggctgg
     9421 aatactataa aacacaagga tttcctccgc gatgtgatcg ctgcctttaa aaaagaaggc
     9481 atccgtactt ccattttcgt tgatccggat gtggccatgg taaaaaatgc cgccgaaacc
     9541 ggtaccgacc gcattgagct ctacaccgag gcctatgcgc ggaattatcc tgttgaccgt
     9601 gctgaagcgc tcgcccccta cctcgaagcc gctaaagctg cctgcgaagc aggactggga
     9661 atcaatgccg gccacgacct cgacctgaac aacctgtggt atatcgcccg ttcacttccg
     9721 ggcctgcagg aagtttcgat cggccatgcc ctgatcagcg aagcgctgta tctcgggctg
     9781 gaaaaaacca tcgggctgta cctgcaacaa ctgaaggaat aagctggtga attgtttacc
     9841 cccttacctt atgaaatacc ccgccaccat tattctgatc cttctgctcc aggcgtttgt
     9901 cagtgcccag ggtgttgtgg acgcttccaa actctcagaa cgtgaaaaca tcatgtatct
     9961 cagcgggcag gatacgcctt tttcgggaac ggctgtcgtt tggctttcgc cgggacaaaa
    10021 acaggttgaa tcgcagtacc tcgacggcag gctcaatggc gtggaaacca cctgggacaa
    10081 tcagggaaat aaatttacgg aagtcacttt caggaacggg gctttcaatg ggccctataa
    10141 acaatggtac cccaacggac agcttgaaat tgataaaatt taccgggatg gcatgatgaa
    10201 cggcccttcg gtgcactatt acgataacgg gcagaaggaa aatgaaggcg tttacctgga
    10261 ttgccgcgaa acaggaacct ggacattcta ttacaggaac gggcaaaagc tgaaagaagg
    10321 cgaatttgtg aacggttttg aagaaggcgt ctggaagtat tatcaggaaa acggaacctt
    10381 atcgaaagaa gtaatataca gtgacgggaa tgtagttagt gaaaaatcat tctgaaaaag
    10441 gaggtcctca tgaaaaccat gctcagaatt gttctcgctg tttgctgctt ttcaggtttt
    10501 aatgtatggg gccagaaggc agaaatttcg aaagctgtcg tcaaaaaatg ggagacaccg
    10561 gcccggctga aaacgcccga atcggttcta tacgacggca aagacgttat ctatgtggcc
    10621 aatatcaacg gccagcccgc agccaaagac ggcaacggtt ttatttccaa aatttcatta
    10681 gacggcagga ttctgaagtc agaatggatc acgggcctgg atgcgcccaa aggcatgggc
    10741 ctttattccg gaaaactgta tgtaaccaac atcaccgaag tggttgaaat cagcattgcc
    10801 gggagcaaaa tcacaaaacg ttacccggcc gaaggatcgg ttttcctgaa cgatataagt
    10861 atcagccctt ccggggccgt atatgtttcc gattcgcgga aagggactat ttatcgtttg
    10921 atgaacggta aacttgaaaa atggatggaa gggctggacg gcgtcaacgg tttgtattgt
    10981 acgggaaaag acatgctgct ggccggaacc gggaattcca tcgttcagat caacacagcc
    11041 ggtaagcaaa aatccacgct ggtttccggg accgggagca tcgacgggct gatatactgc
    11101 ggtaagaaca catatctctt ttccgattgg gagggatcgg tttaccgcgt ggttatgggc
    11161 aaggcaaagg aattgctgtt gaacacctcg aaagcaggca tcaatgctgc cgatatcgat
    11221 tatattccgt cgaaacgcct gctgcttgtc ccgacattcc gggataaccg ggtgatggct
    11281 tacgaactga aataatgccc tgagggtgat atgttgagtt actgcaaata acgctaattc
    11341 caatctgcct tcgttgcccg atccggctga atttcatagc cagcctctcc ggtttcgtaa
    11401 tatttttaac caccattcga acacccgtga taaactggga aatggcaatt tgcaggaagg
    11461 ttagtaatat ttatatttcg gaacagatgt ccgccgactg gcggataaat agtcaaccgg
    11521 gaataaaagc cggcgtcacg aaagacggga caggacccgt acacatctca tattcctgtt
    11581 tatcatgaga cgaacgaacg ctgccaattg tctcattata tgtttttaaa tataataatc
    11641 cactagtggg cattaaaaat acttaatcgt tctttgaaag gctttgtaaa tcgcagtttc
    11701 agcgtttttt aggtgcgtga cgatttgaac tgaatgatgg attttgctgg aaatagctta
    11761 tccatgaatc aaggcaaaaa catcttcgct cagctcacag ggtttctacc tcgtcgggtt
    11821 tttgatcgga tcgttaaaga gttcaacggc aacaggtaca taaagacttt tacttgttgg
    11881 aaccagatgc tctgcatggt atttggccaa ctaactgcaa gggatagtat gcgggattca
    11941 attctcagtt tggaagcaca ccaggctgag tattatcatt tgggatttgg ttcaaaagtc
    12001 tcgcgccgca accttggcac cgccaatgaa aaacgtagct gtaaaatatt tgacgagttt
    12061 gcctgcgttt taatggttca agcccgacga agctcgtatc gatcagttt
//
LOCUS       DAOPYV010000083        11595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_6332_length_11595_cov_12.451906, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000083 DAOPYV010000000
VERSION     DAOPYV010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11595
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6332_length_11595_cov_12.451906"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1146)
                     /locus_tag="PKI35_10010"
     CDS             complement(73..1146)
                     /locus_tag="PKI35_10010"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNX44760.1"
                     /translation="MKERLFVFILLVFTGNQLFSQIPEGYYNTASGLYGTNLQQALHD
                     IIDNHTSVTYSSLWTYFQDTDKKPDGKVWDMYSDVPGGTPAYEYTFITDQCGNYNSEG
                     DCYNKEHSFPASWFNDGYPMYSDMFHLVPTDGYVNNKRSNYPFGEVGTASWTSTNGSK
                     LGNCVSTGYSGTVFEPIDAYKGDFARNLFYMATRYYKEDTGWPGSDMVSGSQPKPWAI
                     AMLLEWHQADPVSQKEIDRNNAIYAIQHNRNPFIDHPEYAANIWSPNALAEEGVKAQH
                     LITIFPTPSSGRCYFDLPEGSESKDIALCVHSASGTSVTPAFAINNTTVTVNLDNMAA
                     GMYVLVLINNQTGRHYIGKILVH"
     gene            complement(1159..1722)
                     /locus_tag="PKI35_10015"
     CDS             complement(1159..1722)
                     /locus_tag="PKI35_10015"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HNX44761.1"
                     /translation="MNLLTIMILAIAVALVTFPTYITSGVILIHEKSGSKLRFIAIMV
                     SVQALMLGLGIVLGMNVGRISEASNKLMALSILMIFGLKVLLDSLRVRPQEKAFDTSD
                     LKVLMLLSLAESIVPMAVAIAVGLMTETFLTPWLSFIAMQGIAIVGGLFTGGQMGTAS
                     FKLRTGPIGGLFLIAASLQMIISMISY"
     gene            complement(1758..2222)
                     /locus_tag="PKI35_10020"
     CDS             complement(1758..2222)
                     /locus_tag="PKI35_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNX44762.1"
                     /translation="MDTLKKAARYERIFDQLRGLLTKTDEPVARMATVAAVLSHKFEY
                     FFWCGFYRVSGEKLIVGPYQGPVACQVLEDRGVCLEAVKRKSTVIVPNVHEFPGHIAC
                     DSRSNSEIVVPVTGTGGDTLAVLDVDSKDFNAFDDTDAKYLGKIVLLIYQAG"
     gene            complement(2391..3770)
                     /locus_tag="PKI35_10025"
     CDS             complement(2391..3770)
                     /locus_tag="PKI35_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNX44763.1"
                     /translation="MELPFAEPYRIKMVEPIRRSSREEREQWIKDGGYNLFNLRSEQV
                     YIDLLTDSGTGAMSDRQWSEMMIGDESYAGSSSYYKLKAAVKDIFGFDYFLPTHQGRG
                     AENVLFSVLVKPGDIVPGNSHFDTTKGHIEFRHATAIDCTIDEAFDTAVNHPFKGNVD
                     LEKLEAVLKKNPRERIPFIIVTVTCNSSGGQPVSMENLKGVHDMAKKYGIPVIYDSAR
                     FAENAYFIKVREKGQENRSIREIVSEMFSYADGMTMSSKKDGIVNIGGLIGLRSQELF
                     GQASVYNIMYEGFITYGGLAGRDLSALACGLYEATDFEYLDSRIKQIEHLGKKLTDYG
                     IHVQMPYGGHAIFVDANKFLPHIPKEEFRAQTLAVELYTESGVRGVEIGALLADRDPI
                     TRENRYPALELLRLAIPRRVYTFNHMDYIAASLANVYDRRNEIKKGLVIDYEAPILRH
                     FTVRLKRAQ"
     gene            4021..5400
                     /locus_tag="PKI35_10030"
     CDS             4021..5400
                     /locus_tag="PKI35_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44764.1"
                     /translation="MKNIQNLLYTGMILTTVLTACHSAGKGNLQNLQKGCVTAKVCCA
                     ADTSVNYSLYIPSGYDPGREWPLIIAFDPHAAGLIPVSLFKDEAEKRGFLVAASNNSK
                     NGMDFGESTAIYHTMLDDIKSRFSTDTGRIYTVGFSGGARVAGMLAMTEGNIAGVVSC
                     GAGLPSSGRPPQKPFSFLGVAGTEDFNYPELLSLDPQLEQAGFTHYLLTFEGKHEWPP
                     KELVPDIMTWLEFDAMRTSHLPVDRNAINRFIDRNDSLDNTFATAGKSYERWQAYEKM
                     QHYLRGLTDVSSLENILATLAQDTKVIERKKQMEDQLMLEDNLRKLYSEKLQNPDLNY
                     WQAESAKLRRLSARQPPDDVTVVYKRILGFLSLSSYMYSNNALKQNLPDAAGIYIEIY
                     RLVDPENPEHRYMAAVAAARKGDKEAAFGYLNKAVESGFKEKSRLLSDQDFTEYHSDP
                     RFRALLKRF"
     gene            5550..6818
                     /locus_tag="PKI35_10035"
     CDS             5550..6818
                     /locus_tag="PKI35_10035"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HNX44765.1"
                     /translation="MKKLLLIIGLLAGMSAQAAFTDWFMDKTLRIDYYHSGDYSNEFY
                     SIDQLRMEPYWGGSKTFLIDTTNYGSYYVKVYDRKSGTLIYSHGYCTLFDEWKFTSEA
                     RQTQRTFSETVTMPFPKDDIIVDFYSRSGHGVYEKKFSYPVNPKSPYIASERQQEYPI
                     YEALNSGDPAMKVDIIIIPEGYTAGEMDRFKADCDKFTEELFTFMPYTLNRDKFNIRG
                     VLAPSAESGTDIPADNVWKKTIVNSSFYTFESERYLMTMDNHSVRNLAANAPYDQIYI
                     LVNSKKYGGGAIYNFYNVSVNSNASSAKIFVHEFGHGFAALADEYDDGSTAFNDMYPL
                     GVEPWEPNITTLVNFGAKWQNMLPENTAVPTPVEGAAPLQPGVYEGAGYVAKGIYRPV
                     PDCLMRTFRGKDFCPVCTKAIQDMIDRYTH"
     gene            6960..7862
                     /locus_tag="PKI35_10040"
     CDS             6960..7862
                     /locus_tag="PKI35_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNX44766.1"
                     /translation="MAITKLDQLFDLLKNKSRKRLVAAYANDAHTIEAVYSAVEKGII
                     NATLTGDETNIKKICQEHGLDVSKLEIVHEPNELKAAALAVQLINEGKGNILMKGSLS
                     TDKYMRAILNKEKGLCNEGAVLSHVTIMEYPSFDRLLTIGDVAVIPLPDFKQKIKMVG
                     YLVKTAKAIGVEMPKVAAIAATEQMLAGMPACVEGAMLAKMNDRGQIAGCILDGPLSV
                     DCAIDKESVEIKKLAGPVAGNADCLLFPNIESGNTFYKGGTKLAGAELAAYVAGARVP
                     CVLSSRGDSARTKAYSIALAALMA"
     gene            7878..8954
                     /gene="buk"
                     /locus_tag="PKI35_10045"
     CDS             7878..8954
                     /gene="buk"
                     /locus_tag="PKI35_10045"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNX44767.1"
                     /translation="MDDFRILAINPGSTSTKIAVFHNANPLFVKNITHSAEELAPFEH
                     IADQFSFRKEIIYRELQNADIQLDLIRAIVGRGGMLKPIASGVYEVNDAMKNDLHFHP
                     IKEHASNLGGLIADEIARSLPDAKAYIADPVVVDELDDIARVSGHPAFKRISIFHALN
                     QKSIARQHAKSIMKRYEDMNLIVVHMGGGVTVGAHEKGKVIDVNQGLDGEGPFSPERS
                     GTLPSGDLARLCFSGKYTEKEVLKMIVGNGGIVAHLGTNSVYEAEQRAMNGDEKARFI
                     LEAFAYQVSKAVGSMVPVLKGHVDAILLTGGVAHSKWITNLILERVYSFAPVYIYPGE
                     DEMRALALNGLMVLKGEIEPKVYA"
     gene            complement(9186..9461)
                     /locus_tag="PKI35_10050"
     CDS             complement(9186..9461)
                     /locus_tag="PKI35_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44768.1"
                     /translation="MQNIYFRRAAEKILLVLVLTSATILGNHAMAGTPPPPPPGTSTT
                     GGGHGAGGNKGPSEAPVGNGVGLLMLLGAAYAGGKSYMLRQKEVSEA"
     gene            complement(9485..9748)
                     /locus_tag="PKI35_10055"
     CDS             complement(9485..9748)
                     /locus_tag="PKI35_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44769.1"
                     /translation="MQILQDYHKKHQKSVSLFTGLSINKGRIALMGKGEGVAFTDANI
                     RKGLGEFGFIRFMYGSAYCHVPVSGHLQAYHSSFIGISSLRYH"
     gene            complement(10216..10458)
                     /locus_tag="PKI35_10060"
     CDS             complement(10216..10458)
                     /locus_tag="PKI35_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44770.1"
                     /translation="MKQILLILLFVFSTSVFDLTYATTPPPPPGTPGMGGGHGTGGNN
                     GPTGAPVGRGLEVLMLLGAAYAGNKMYKSKREKERE"
     gene            complement(10516..>11595)
                     /locus_tag="PKI35_10065"
     CDS             complement(10516..>11595)
                     /locus_tag="PKI35_10065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44771.1"
                     /translation="SGYSPSTHYFTGVLNSGDAYPGTVAASRFYLVGNPYSSSIDWQH
                     NMWETSNNPADNHRNGLESTDGTYDYWVFRDGASGGNYLAGNSGGTYSSGLSKDIAPM
                     QGFFVLTKQGGGLFEITNSVRVHSSQSWVKSAAVQNNVLRLELTTNKNSYRDEVMIDF
                     NDQYSGEGGSYKFGSLYYDAPELWSVKNGNNYTINRYKEITPGLAVNISAKCGVDGTY
                     TLTATNISDFSLSDIVYLEDLKTGTKVDLKAVGSYSFAGSPNDNKERFRVTFAEIAGA
                     GEPGADKPVYIYSYENEVYINASRPETGNCAVYIYDALGRVIYRGSYDPVTGNQRFTT
                     LSTPGAYIVKVVSGSGIVTAKIVIP"
BASE COUNT         3071 a         2869 c         2705 g         2950 t
ORIGIN      
        1 gcgtgcgcca tttcgaattt gaattctttt gtcagcctga ctgttgtcac tatctattgt
       61 attaggtatg atttaatgaa ccagaatttt cccaatgtaa tgcctgccgg tttgattgtt
      121 gatcagtaca agcacataca tccctgcggc catattatcc aggttaaccg taactgttgt
      181 attattgatg gcaaaagccg gcgtaacaga cgtaccgctt gccgaatgca cacacagggc
      241 gatatccttg ctttcggacc cttccggtaa atcaaagtaa cacctgcccg atgacggcgt
      301 cggaaaaatc gtgattaagt gctgcgcttt gacgccttct tcagccaaag cattcggcga
      361 ccaaatgttc gctgcatatt cgggatgatc gataaaaggg ttccggttgt gctggatggc
      421 gtaaatggca ttattacggt caatttcttt ctgcgagaca gggtctgcct gatgccactc
      481 cagcagcatg gcgatagccc agggcttggg ttgggaaccg ctgaccatgt cgcttcccgg
      541 ccaaccggta tcttccttgt aataccgcgt tgccatatag aacaaattcc tggcaaaatc
      601 gcctttgtaa gcatcaatgg gttcaaatac ggttcccgaa taaccggtag aaacacagtt
      661 gcctaattta ctaccattgg tcgatgtcca cgaggctgta ccgacttcac cgaaaggata
      721 atttgaacgt ttattgttca catatccatc cgtaggaacc aggtgaaaca tatcggaata
      781 catcgggtaa ccgtcattaa accagcttgc cgggaatgaa tgctccttgt tgtaacaatc
      841 gccttcgctg ttatagtttc cgcactgatc ggttataaat gtgtactcat aggcgggagt
      901 tccgccggga acatcggaat acatgtccca gacttttccg tcaggctttt tgtcggtatc
      961 ctggaaataa gtccagagcg aactgtaagt taccgaagta tggttgtcga taatatcgtg
     1021 aagcgcctgc tgtaaatttg ttccgtataa tcctgatgcc gtgttataat agccttcagg
     1081 aatttgagaa aacaactgat ttccggtaaa aacaagcaaa atgaatacga acaatctttc
     1141 cttcatgttt ttttaagttt aataactaat catgctgatg atcatctgca gtgaggcggc
     1201 aatcaggaat aacccgccta ttggcccggt cctgagttta aacgacgctg ttcccatttg
     1261 cccgccagta aataatccgc ctacaatggc tattccttgc atggcaatga acgacagcca
     1321 gggagttaaa aaggtttccg tcatcagtcc gacagctata gctacagcca tcggaacaat
     1381 ggactccgcg agtgaaagca gcatcagcac ctttaaatcg cttgtatcga aagctttttc
     1441 ctgcgggcgt acacgcaacg agtcgagcag cactttcaga ccgaaaatca tcaggataga
     1501 aagcgccatc agcttgttgg aggcttccga aatacgcccg acgttcattc caagcacgat
     1561 tcccaatccg agcatcaggg cctggacaga aaccattatt gcgatgaaac gcagtttact
     1621 tcctgacttt tcatggatga gtatcacacc gctggtgatg taagtcggaa atgtcaccag
     1681 ggcaacggct atcgccagga tcatgatggt cagcaaattc atggtgtaca gataatatgg
     1741 ttatatcagg cctttcgtta accggcctga taaattaaca agacaatctt tccgagatat
     1801 tttgcatcag tatcatcaaa ggcattaaag tctttgctgt caacatccag cacggccagg
     1861 gtatctcccc cggttcccgt tacgggtact actatttcgg aattcgatct tgaatcgcag
     1921 gctatatgcc ccgggaattc atgcacattc ggaacaatga ctgtactttt tctcttaacg
     1981 gcttcgaggc acacgcccct gtcttccagt acctggcagg cgacaggtcc ctggtatggc
     2041 ccgacaatca acttttctcc gcttaccctg taaaatccgc accagaaaaa gtattcaaac
     2101 ttgtgactca gcacggctgc cacagtcgcc atccgggcaa caggttcgtc agtttttgtc
     2161 agcaagcccc ggagctgatc aaaaatcctt tcatatctcg ccgccttttt aagggtatcc
     2221 atgtgcaaat atttgctgca aaggtaatta ataccgtcca gcagtccttt cgttcatttc
     2281 acaaatataa ggcgggtttg ccgaatggaa cgtttctgtt gcatccggca gcagaaaaaa
     2341 agcctccgga agcatgattc ccggaggctt ttaacaatgt caggattcgt ttattgagcc
     2401 cttttgagcc tgactgtaaa gtgcctgaga atcggcgctt catagtcaat aaccaaacct
     2461 ttctttattt catttctgcg gtcgtaaacg ttggccagcg aagcagcaat gtaatccatg
     2521 tgattaaagg tatataccct ccggggtatt gcaagcctga gcagttccag ggcaggatac
     2581 cggttttcac gggtaatggg gtcccggtcg gccagtaagg ctccgatctc aacgccacgc
     2641 acaccgcttt cggtgtaaag ttccacggcc agcgtctgag ccctgaattc ttccttcggg
     2701 atgtgaggca ggaacttatt ggcatcgaca aaaatagcat gcccgccata cggcatctga
     2761 acgtgaatcc cgtaatcggt aagctttttc ccaagatgtt ctatttgttt tatcctggag
     2821 tcaaggtatt caaaatcggt tgcttcgtat aacccgcatg caagtgcgct taaatcacga
     2881 ccggccaatc cgccgtaggt gataaaccct tcgtacataa tattatacac cgaagcctgc
     2941 ccgaacaatt cctggctgcg caatccgatc agtccgccga tattcacaat gccatccttt
     3001 ttgctgctca ttgtcattcc atcggcatag gaaaacattt cagagacgat ttcacggatg
     3061 ctgcgatttt cctggccttt ttcgcgcact ttgataaagt atgcattttc ggcaaagcgt
     3121 gcggaatcat aaattacggg gatcccgtat tttttggcca tgtcgtgaac gcctttcagg
     3181 ttttccatcg ataccggctg ccctcctgat gaattgcagg tgacagtaac aatgataaac
     3241 ggaatacgtt ccctgggatt ctttttgaga acggcttcca gtttctcaag atccacgttt
     3301 cccttgaaag ggtgattgac ggcggtgtca aaagcttcat ctatcgtgca gtcgatagct
     3361 gtagcatgac ggaattcgat atgccctttg gtggtatcaa aatgtgaatt cccggggact
     3421 atgtcgccgg gtttcaccag aacgctgaaa agtacgtttt cggcgcctct tccctgatgc
     3481 gtagggagga agtaatcgaa tccgaagata tccttaacgg cagctttcag tttgtaatac
     3541 gacgacgatc cggcatagct ttcatcgccg atcatcattt cgctccattg ccggtcgctc
     3601 atggcgccgg ttcccgaatc ggtgagcaga tcgatgtaca cctgttcgct gcgtaaattg
     3661 aacaggttgt agccgccatc tttaatccat tgttcgcgct cttcgcggct gcttctgcga
     3721 ataggctcaa ccatctttat tctgtaaggt tctgcaaaag gtagttccat tattttattt
     3781 tcaatgttaa cattaataat tgattacttt agattggata gtaaaagata tgaccggaaa
     3841 ttatcccggt ccttgaggtt taaaaatatt tttttctttg aaacggttgt gctcattttc
     3901 aacctccatc aaacgagaaa tggaacgcgc caaatataca tcattttgaa cgaaaataaa
     3961 aaatacagac ttttgcatac ttcagggaac ggaaatgcta tttgttataa aatcctcagc
     4021 atgaaaaata tacaaaatct gctttatacc ggaatgatct taacaacagt ccttacggca
     4081 tgccattcag cgggaaaggg aaatctgcaa aaccttcaaa aaggatgcgt taccgctaaa
     4141 gtatgctgcg cggctgatac ctcggtaaat tactcccttt atattccttc cggatatgat
     4201 ccaggcaggg aatggcccct gatcatcgca ttcgatccgc atgctgccgg gttgattcct
     4261 gtcagccttt tcaaagatga agcagagaaa agaggtttcc tggttgccgc ttccaacaat
     4321 tcaaaaaacg ggatggattt cggggaatcg acagctatct atcacacgat gctcgatgat
     4381 atcaaatccc gattttcaac agataccggc cggatttaca ctgttggctt ttcaggcggg
     4441 gccagggtcg ccgggatgct ggcaatgacc gaaggcaata ttgcaggggt agtaagctgc
     4501 ggtgcaggac ttccctcgtc aggcaggccg ccgcaaaagc cctttagttt cctgggcgtt
     4561 gcaggtactg aggatttcaa ctatcccgaa ttactgagcc tcgatccgca actggaacag
     4621 gccggtttca cccattattt gctaacattt gaaggcaaac atgaatggcc gcccaaagag
     4681 cttgtaccgg atattatgac ctggctggaa tttgatgcca tgcgcacaag ccacctgcct
     4741 gtcgacagga acgccatcaa ccgttttatc gacaggaacg acagcctgga caacactttc
     4801 gcaacagcag gaaaatcata tgaacgatgg caggcgtatg agaaaatgca gcattacctc
     4861 cgtggattga cggatgtatc gtctctggaa aatattctgg caacactggc ccaggatacg
     4921 aaagtgattg aacggaaaaa acagatggaa gatcaactga tgctggagga taatctcaga
     4981 aagttgtatt ccgaaaagct tcagaatcct gatctgaact attggcaggc tgaatcggca
     5041 aagctcagga gattatccgc caggcaaccg cctgacgatg tgacggttgt ttataaaagg
     5101 atactgggat tcctcagttt gtcgtcgtac atgtattcaa acaatgcact gaaacaaaac
     5161 ctccccgatg ccgccggtat ctacatcgaa atttaccgtc tggtcgatcc cgaaaatccg
     5221 gagcaccggt atatggcggc tgttgcggcc gccaggaaag gcgacaaaga ggctgcattt
     5281 gggtatttga ataaagcggt tgaatcaggc ttcaaagaga aatcaaggtt gctgtccgat
     5341 caggatttta ccgaatatca ttccgaccct aggtttaggg ccctcctgaa aaggttttag
     5401 ggttgccccc gcccctaaga aagatcgtcc gcggtgttgc ctgaccaggc gccagtatcg
     5461 tcccggggtt caaatccgtt ttgccggcac ctgccttctg agatttcatt tatttgtaat
     5521 ttcgtgggca tcaatcagaa ttctttacca tgaaaaaact ccttctcatc atcggattac
     5581 ttgccggcat gtcggcacag gcagctttca ccgactggtt catggataaa accctgcgta
     5641 tcgattatta ccattcgggc gattattcga acgagtttta ctccatcgac cagctaagaa
     5701 tggaacctta ttggggcgga tcgaaaacgt tccttatcga cacgaccaac tatggctcat
     5761 attatgtaaa agtatatgac aggaaatcag gaacactgat atacagccat ggttactgca
     5821 cgcttttcga cgagtggaag tttacatccg aagcccgcca gactcagcgc actttcagcg
     5881 aaaccgttac catgcctttt ccgaaagatg atatcatcgt tgatttttac agcaggtcgg
     5941 gtcacggggt gtatgagaaa aagttcagct atccggtgaa cccgaaaagc ccttacattg
     6001 ccagtgaacg ccagcaggaa tacccgattt acgaagcgct gaacagcgga gacccggcta
     6061 tgaaagtgga tatcatcatc attcccgaag gctataccgc tggagaaatg gaccgtttta
     6121 aagccgattg cgataaattc acggaagagt tgttcacttt tatgccttat accctcaacc
     6181 gcgataaatt caatatccgc ggcgtgctgg cgccatcagc cgaaagcggc accgatattc
     6241 ccgccgacaa tgtgtggaag aaaaccattg tcaattcaag tttttatact ttcgaatcgg
     6301 aacgttacct gatgaccatg gacaatcaca gcgtccgcaa ccttgcagcc aatgctccgt
     6361 acgaccagat atacattctg gttaacagca agaaatacgg gggcggcgcc atctacaatt
     6421 tttataatgt ttcggtcaac agcaacgcct catcggccaa gatttttgtt catgagttcg
     6481 gtcacgggtt tgccgctctt gccgacgaat acgacgacgg ctccaccgct tttaacgaca
     6541 tgtacccgtt gggtgtggaa ccctgggagc ccaacatcac tacgctggtt aatttcggag
     6601 caaaatggca gaatatgttg cccgaaaata ctgctgtacc cacgccggtt gaaggagcgg
     6661 cccctttgca gcccggcgtt tacgaaggag ccggttatgt agccaaaggc atttaccgtc
     6721 ctgttcccga ttgcctcatg cggaccttca gagggaaaga tttttgcccg gtttgcacca
     6781 aagctatcca ggatatgatt gacaggtata cacattaaat tatatgtctt ctaaataaga
     6841 ctaatgaccg agaaaaccct tcagaaatga agggtttttt ataattttgc catcgaaatg
     6901 gccgggaacg gattcattat cagatcatat aagattatta acttcaaaac aatatcatca
     6961 tggcaatcac aaagttggat cagctcttcg atttgttaaa aaataaatcc cgtaaacgcc
     7021 tcgtggcagc ttatgccaac gacgcccaca ccatcgaagc agtgtactcc gctgttgaaa
     7081 aaggaatcat caatgccacc cttaccggcg acgaaacgaa cattaaaaaa atatgccagg
     7141 agcacgggct ggatgtttct aagttggaga ttgtgcatga gcccaacgaa ctaaaggctg
     7201 cagctctcgc cgtacaactc atcaacgagg gcaaagggaa tattttaatg aaaggttcgc
     7261 tgagtaccga taaatacatg cgcgctatcc tgaacaagga aaaaggattg tgtaacgaag
     7321 gagccgtgct cagtcatgtt accatcatgg aatacccgtc ctttgacagg ctgctcacga
     7381 ttggcgatgt cgcagttatt ccccttcccg attttaaaca aaagattaaa atggtgggct
     7441 atcttgtaaa aactgccaag gccattggcg ttgaaatgcc taaagttgca gccatcgcag
     7501 ctaccgagca gatgctggcc ggcatgcctg catgcgttga aggagccatg ctcgccaaaa
     7561 tgaacgaccg cggccagatt gcaggttgta tcctggatgg cccgctcagt gtagattgcg
     7621 ctattgataa ggaatctgtc gagataaaga aattggccgg tccggtcgcc ggaaacgccg
     7681 attgcctttt gttcccgaat attgaatccg gcaacacatt ttacaaggga ggcaccaaat
     7741 tagccggagc tgaacttgcc gcctatgtag ccggcgccag ggttccctgt gtgctctcgt
     7801 cacgcggcga cagcgcccgc acaaaagctt attccatcgc ccttgccgct cttatggcct
     7861 aaaagctgcc gaccatcatg gacgatttcc gaatccttgc catcaatccc ggctccactt
     7921 ccacaaaaat agcggtgttc cacaatgcta acccgttatt tgtcaagaac atcactcatt
     7981 ctgccgaaga actggcccct tttgaacata tcgccgacca gttttcgttc cgcaaagaaa
     8041 ttatttacag ggaattgcag aatgcagata ttcaacttga cctgattcgc gccatcgtgg
     8101 gccgcggagg catgctcaaa cccattgcct cgggcgttta cgaagtgaac gatgccatga
     8161 agaacgatct tcatttccat cccataaagg aacacgccag caacctgggc ggactgatcg
     8221 cagatgagat tgcccgttca ctcccggatg ccaaagccta catcgctgat ccggtcgtgg
     8281 tggacgaact cgacgacatt gccagggtat caggacaccc ggcattcaaa agaatttcca
     8341 tttttcatgc cctgaaccag aagtctattg cccgtcagca tgccaaaagc atcatgaaac
     8401 ggtatgaaga tatgaacctt atcgttgttc acatgggcgg aggcgttacg gtaggagcgc
     8461 atgaaaaagg aaaagtcata gacgtcaacc aggggctcga cggagaagga ccattttcgc
     8521 cggagcgcag cggaaccctg ccttcaggcg atcttgcaag gctctgcttc agcggaaaat
     8581 ataccgaaaa agaagtgctg aagatgatcg tcggcaatgg cggcatcgtt gcccaccttg
     8641 ggacaaacag cgtttacgaa gcagaacaac gggcgatgaa cggcgatgaa aaagcgcgtt
     8701 ttatcctcga agcttttgca taccaggttt caaaagccgt cggttcaatg gtgcctgtcc
     8761 tcaaaggtca tgttgatgca attctgctta caggcggcgt tgcccacagc aaatggatca
     8821 cgaaccttat cctcgaaaga gtttattcct tcgcacctgt ttacatctac cccggcgagg
     8881 acgaaatgcg cgccctggcc cttaacggac tcatggtgct caaaggtgaa attgaaccta
     8941 aagtatatgc ctgacaatta aatatatacc aggcttcata ctaacaaaac agcaggcaat
     9001 ggcctgcttt tttgttaatc ccggctatgt tcctttcatt cctgatgctg aaacgggatt
     9061 attaaaggcc ggggaggaaa agaaaaaaca ttccgtcacc ggctgacaat caagaaattt
     9121 gcgtcagcaa ggatgttatc agcaagcagg aggaaaccaa tcgcaaggat aaataccgga
     9181 aaacgctatg cctctgatac ttccttttgc ctgagcatat aacttttacc tccggcatag
     9241 gcggctccga gcaacatcag caacccgacc ccattgccaa caggagcttc ggaagggcct
     9301 ttgttccctc cggcgccatg tccgccgccc gtggttgatg ttccgggagg gggcggtggc
     9361 ggagtgccgg ccatagcatg attacccaat attgtcgcag aagtcaacac caatacaagg
     9421 agtatctttt ctgccgcccg tcggaagtaa atattttgca taggatatgt taaagaaaac
     9481 cttttcaatg atatctcaag gaagaaatac cgataaatga ggaatgatac gcttgcaggt
     9541 gtccggaaac cggcacatga caatatgcag acccatacat aaatcttata aatccaaact
     9601 cccccagtcc ttttctgatg ttggcgtctg taaaagctac accttcccct ttccccatta
     9661 aggcaattct tcctttatta atggacaggc cggtaaaaag tgatactgat ttctgatgtt
     9721 ttttatgata atcctgcaaa atttgcatga gcggcctgct atccccggaa tatgatgata
     9781 cctgacctga tttgtacccg gtttgtggaa ccttttacgg gatccgggct acccacaaaa
     9841 tacagcgctt acaaaggaac ctatctcccc gaaggccagt caggcggcag gcaccaaagg
     9901 ctgacaaaga aggcttatct cagaatagtt gcgccggttt tgggatgcgc gtttttttac
     9961 cagggtccgg caaccaggat caggaagccc gaaattcccc ggaaaccggc attcgtgtcc
    10021 gctcagagat atggcgtgcc ggcagcagga acggccgcca tccggcttat tcggcaagtt
    10081 acagaaagaa ggcccggatt tcaccaaaaa agcagcatcc atcgctgaat gctgcctgta
    10141 tcaggggtaa aagtctgctg tcaaaacatt aactgaaaat ccaggcaaaa cattcgcctg
    10201 cacacgttag cctcttcatt ctctttcctt ttcccgtttc gacttataca ttttattacc
    10261 ggcataggcg gcgccaagca gcatcaatac ttccagcccg cggccaacag gcgctcccgt
    10321 tggcccgttg tttccgcctg ttccatggcc gccccccata ccgggagtgc ccggaggcgg
    10381 cggaggcgtg gtggcataag tcaagtcgaa aaccgatgtt gaaaacacaa ataataaaat
    10441 gagaagaatc tgtttcattg cccggatcct gttgtttagg tgttgagtat ccatatcaat
    10501 ttggttgata atctgttagg gtatgacaat ttttgcagta acgattccgc tcccggaaac
    10561 caccttcacg atataggcgc caggggtcga aagcgtggta aacctttgat tgcctgtaac
    10621 cggatcatag ctgccgcggt aaatcacccg gcccagggcg tcgtaaatat atacggcgca
    10681 atttccggtc tcaggacggc tggcgttgat gtaaacctcg ttctcgtaac tgtagatata
    10741 taccggttta tcggcgccag gttcaccggc gccggcaatt tctgcaaagg ttaccctgaa
    10801 acgttccttg ttgtcgttgg ggctgcctgc aaaggagtat gaacccacag ccttcaggtc
    10861 taccttcgtt ccggttttca ggtcttcgag ataaacgata tcagataggc tgaaatcgct
    10921 tatattggtg gccgtaaggg tataggtacc gtcaacgccg catttggcgc tgatattcac
    10981 ggcaaggccg ggggtgatct ccttgtaccg gttgatggtg taattgttgc cgtttttcac
    11041 cgaccacagt tccggagcgt cataatacag gctgccgaat ttataggaac caccttctcc
    11101 ggaatactga tcgttgaaat cgatcattac ttcatccctg taactattct tgttggtggt
    11161 taactccagc ctcagcacat tgttttgaac ggccgccgat tttacccacg attgtgaact
    11221 atgcacgcgg acagaattcg taatttcgaa taatccgccc ccttgttttg tgagtacgaa
    11281 gaacccctgc atgggggcaa tatccttgct cagccctgaa ctataggtgc ccccggaatt
    11341 acccgccagg taattcccgc ccgatgcgcc atcgcggaac acccaataat cataagtgcc
    11401 atcggtgctc tccagaccgt ttcggtggtt gtccgccgga ttgttcgagg tttcccacat
    11461 attgtgctgc cagtcgatgg aggaggaata aggattcccg accaggtaaa aacggcttgc
    11521 ggccacagtg ccgggataag catccccgga attcaggacg cctgtaaaat aatgcgttga
    11581 aggtgaataa cctga
//
LOCUS       DAOPYV010000084        11382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_6502_length_11382_cov_12.892734, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000084 DAOPYV010000000
VERSION     DAOPYV010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6502_length_11382_cov_12.892734"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..981)
                     /locus_tag="PKI35_10070"
     CDS             complement(157..981)
                     /locus_tag="PKI35_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX44772.1"
                     /translation="MKKVIAFLSLAVMLTIGLPNYTFAQDQAAPATTATVDSAAPQVD
                     SAAAPVADQTTAAPVEESKSFHQMLKQKYIEGGPGWMAPILLCLIIGLGLCIERIIYL
                     NLASTNTSKLLEKVESELQTNGVSAAKEVCKNTRGPVASIFYQGLDRYHEGIEIVEKS
                     IVSYGGVQMARLESNLSWITLFIALAPMLGFLGTVVGMVQAFDAIETAGDISPTVVAG
                     GMKVALITTVFGLIVAIILQVIYNYLVSKVESIVNSMEDATISFMDILVKNKNAKN"
     gene            complement(1057..1144)
                     /locus_tag="PKI35_10075"
     tRNA            complement(1057..1144)
                     /locus_tag="PKI35_10075"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1108..1110),aa:Ser,seq:gga)
     gene            complement(1261..2286)
                     /locus_tag="PKI35_10080"
     CDS             complement(1261..2286)
                     /locus_tag="PKI35_10080"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I asparaginase"
                     /protein_id="HNX44773.1"
                     /translation="MKSISILVINTGGTIGMVTDKETGALIPFDFGNLYQMMPALGNF
                     DYTIDSYTFTPLIDSSNVTPAFWIELASVIEKNYENYDGFVVLHGTDTMAYSASMLSF
                     MLENLNKPVIFTGSQLPIGVVRSDGRDNFITAVEIAADRKYDTPVVPEVAIYFENKLL
                     RGNRTNKFNAENFNAFLSGNYPPLAEVGVYIRYNHNSILKPNFRSLKVHYSLDTHVAV
                     LKLFPGISEIIVKSVLNIDGLKGVILETYGAGNAPTDKWFVASLQEAIKQGITIYNVT
                     QCKGGSVEMGKYETGLALEKIGVVGGYDITTESAVTKMMFLLGQGISGQKLKELLQTP
                     LRGELTL"
     gene            complement(2371..3144)
                     /locus_tag="PKI35_10085"
     CDS             complement(2371..3144)
                     /locus_tag="PKI35_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNX44774.1"
                     /translation="MYIDTHTHLYLEEFSADCEQAVERAVGLGVEYMLLPNIDSTSWA
                     PMLSLAGRYPQRFFCMAGLHPTSVQPETIEEELNAVEKHLESGKFIAVGEIGMDLYWD
                     KTHLELQEQVFRHQLKLAKRYKLPVAIHVRNSYNEIWRILKQELTDDLKGVFHCFPGN
                     EIQARQVIEAGFLLGTGGVVTFKNSLLQKVIPSVGLQHIVLETDAPFLPPVPYRGKRN
                     EPSYIPLIAAKVAELCCTSVEEVAALTSRNAIKLFNLPL"
     gene            complement(3158..3793)
                     /locus_tag="PKI35_10090"
     CDS             complement(3158..3793)
                     /locus_tag="PKI35_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX44775.1"
                     /translation="MIQIKRFVFNAFGVNGYVLYDETSECLLIDTSCVSENEKDDLDR
                     FISQQGLRPVKLINTHLHVDHVLGNNYICNKYRIGYYAHRKGIPVLETAPQYGALFGV
                     EISPVIQPSGFLEHGDMLHFGHSELRVAYTPGHADGSICLICDTGKFVITGDVLFQNS
                     IGRTDLVTGNFELLRESIYRELFTLPPDFRVLPGHGPETTIGYEKLNNAFL"
     gene            3926..7207
                     /locus_tag="PKI35_10095"
     CDS             3926..7207
                     /locus_tag="PKI35_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44776.1"
                     /translation="MNGKRLVLLIIGIFAGLVLNPFSLRAQFYTGSQMSFGKNRVQYL
                     DYFWTYYKFDKFDVYFYLNGKELALHTARYAESCIPGIEKKLESTIDTKEKIQFLVYN
                     TLNDLRQSNIGLLSNEQYNTGGVTHIIGHKVFLYFDGDLNHLNQQIRSGIAKIMLEKM
                     IYGETLGSQMKSSAVLNLPDWYVEGLVSYLGNEWDTEMDDKVRDGILSGRFLKFNHLK
                     GEDATTVGHSVWYYISQKYGESFIPNIVYMTRVSRGVESGFLYVLGISYKTLMNDWKA
                     WYESKYKEAGAYTSYGPSPSIRIKSDRVYDKVKISPDGEKMLYVCNNNGLYKIFIYDL
                     STGKKHRILRKGYRQDEKTDYTYPLLAWHPGSGVAAMITERKGLLWLYFYDLETRKWT
                     KQNIFGFQKITDFSYSPDGRMFVFSAVQRGESDIFLYNIAANSAEQLTRDNFNDLYPR
                     FLPGTGKIIFSSNRNDDTLRFEKDIPLKYTGQVNNDLFLIDVASKNRKLSRVTNTPLA
                     NEVQAMPYSPGSFTYLSDANGIYNRYVARFDSAIAYVDTATHYRYFTRSIPVTNFPRS
                     ILSQDVSPMAGKSAEVFTEKSRSRICLSDIAAAGPAAAVQLESTPYLQFAKRKYEAQQ
                     AEKDAAKKGQPKVKRKKFSNVYGKEVIPAKDTALIDISNYRIKEITEARSDTSLARRK
                     AAAHETVKDSIPPKVRNYNVEYSINKLISQFDFSFLNASYQPFTGYAGPLYLQPDATV
                     LLSTGATDLLEDYRITGSIKPNFNLKNNEYLISFSNLKSRLDREIFFHRTSYDITYTN
                     ALVRHRIHEIHYIVSYPFTETLCLKGTGMLQSDRTIVLSTDQYTLQADDENNYWASLK
                     GELIFDNTRELGINLYEGMRYKFFGEYYQLIDKKSTNIVIAGCDIRHYTKIHRTFIWA
                     NRLAASTSFGGNKLMYYLGGVDNWISPGFDYDNSIDNSQDYAFQTLATNMRGFKQNVR
                     NGNSFAVWNSELRFPVFKYFINRPLKSDFLNNFQIVGFADFGSAWKGLNPFSEENTYF
                     TRTIYQEPLKVTVQVQKDPFVAGYGFGLRTRLFGYFIRADWAWGIEDGARQDQIFYLS
                     FSLDF"
     gene            7310..7678
                     /locus_tag="PKI35_10100"
     CDS             7310..7678
                     /locus_tag="PKI35_10100"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNX44777.1"
                     /translation="MSLQVILILVLIGLTAGIFGGMVGLGGGVIMIPAMVFLLGMSQL
                     EAQGTSLAIMLPPIGIFAAINYYKAGHIDWRYALIIAAAFTVGGYFGSKIALNIPADL
                     VRKIFAISVIAIALRMFFKS"
     gene            7733..8182
                     /locus_tag="PKI35_10105"
     CDS             7733..8182
                     /locus_tag="PKI35_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNX44778.1"
                     /translation="MPAENIDNRIVSFIHKHHIFTLATIAGNQPYTCTCFYVFMDSLN
                     LFVFTSDEGTRHVAEMKRQKRVAGAVALETKVVGRIQGIQFTGDSFLIEGDVYAKARK
                     AYLKKFPYAGFKELTLWGIEPDFIKMTHNMLGFGTKLIWTKNDDPAF"
     gene            8751..11306
                     /locus_tag="PKI35_10110"
     CDS             8751..11306
                     /locus_tag="PKI35_10110"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNX44779.1"
                     /translation="MPLTQPPKKLITYQHEEVMEAATAYFKGDTLAASVWMNKYALKD
                     SDGNIYERTPDDMHHRLASEVARIERKYPNPMGEEELFELFRDFKYIIPQGSPMAGIG
                     NNFQVSSLSNCFVIGSDENQDSYGGILKVDQEQVQLMKRRGGVGHDLSHIRPYGSAVK
                     NSALTSTGIVPFMERYSNSTREVAQDGRRGALMLSISIQHPDSENFIDAKMEQGKVTG
                     ANISVKIDDDFMKAVKSGSKYTQKYPIGSDNPAKTKEIEAGNLWGKIIHNAWQSAEPG
                     VLFWDTIIRESVPDCYADQGFKTLSTNPCGEIPLCPYDSCRLLAMNLYSYVDNPFTPE
                     ASFNAGLFAKHARRALRIMDDIIDLEMEKIDGILAKIDADPEDEETKRIERKLWENIR
                     SKTLLGRRTGIGITAEGDMLAALGYRYGSDEATNFSVEVHKTLALEAYGSSVDLAAER
                     GPFPIFDIEREKNNPFINRISRLAPEVYQKMLKNGRRNISMLTIAPTGSVSICSQTTS
                     GIEPVFMVSYKRRRKVNPNDRNVTVTFVDEVGDSWEEYNVFHPKFLEWMRVNGYDVDQ
                     ANHLPESEMNALIEKSPYYKATSNDIDWVAKVKMQGAIQKYVDHSISVTVNLPKDVTE
                     ELVSKVYLTAWESGCKGMTIYRDGSRAGVLVSNEPKKEKESSVEFKETKAPPRPKKLE
                     ADVVRFTNESERWIAVVGLLDNKPYEIFTGKSEDFFLPDWVEKGWVIRQKETGKSAMY
                     NFQYLDRQGYKVTYEGLSRTFDKEYWNYAKLISGILRHGMPLPYVVSLVENLTFDKDF
                     ITTWKNGVVRTLSKYIKEARQTGEACPKCGQTDALYRKEGCVYCKHCDYSKCS"
BASE COUNT         3187 a         2748 c         2587 g         2860 t
ORIGIN      
        1 ccagaatccc gatatagaga aggcttccct tggatttccc gaacccctct ttaataaggt
       61 tgtagaaggg gaagaaaatc aaggtggcga ttgcgataaa gaaggctatg tatgcaacat
      121 aaagcccgat ggttatgagg gtatctgaca tagggtttag tttttagcat tcttgttctt
      181 aacaaggata tccatgaagc tgatcgtggc atcttccatg ctgttcacga tggattcaac
      241 ctttgatacg aggtagttgt aaataacctg gaggataata gcaacgataa gaccgaacac
      301 cgtagtgata agagcaactt tcataccacc ggcaacaacg gtcggagaga tatcgcctgc
      361 tgtttcaata gcgtcgaaag cctgaaccat cccgacaact gttcccaaga atccgagcat
      421 aggggccagg gcgatgaaca gggtaatcca tgaaaggttg ctttcgaggc gggccatttg
      481 aacgccacca taagaaacga ttgatttttc aactatttca ataccttcat gataacggtc
      541 aaggccttga tagaaaatgg aagcaacagg tccgcgggta tttttgcaca cttctttagc
      601 agcgctgacg ccattggtct gaagttcgct ttctactttt tcaaggagtt tggatgtatt
      661 ggttgaagca agattcaggt atatgatacg ttcaatgcaa agtcctaatc cgatgataag
      721 gcaaagaaga ataggagcca tccagccggg tcctccttcg atatattttt gcttgagcat
      781 ctggtggaat gattttgatt cttcgacagg tgcagcagtt gtttgatcgg caaccggagc
      841 agcagcactg tcaacctggg gggctgctga atcaacagtt gcagttgttg cgggtgcagc
      901 ctgatcctgt gcgaacgtat aattcgggag gccaatggtt agcattactg ctaatgacaa
      961 gaaagcgatt acttttttca tgggttcact ttattgaatt tgattttttg gtgaattgct
     1021 ttatttaatt tttattgttt caaagaaata acgttgcgga gagaatggga ttcgaaccca
     1081 tgatacgctt ttggcgcata ctcactttcc aggcgagcgc cttagacctc tcggccatct
     1141 ctccgtgata tgcagggctc attgaactta acctcaacag aatcctgcac gattcgggcc
     1201 gctaatttac aaaaaaatct tagaaagtgt tttcgaagac atttttgtaa taaaacccac
     1261 ttaaagtgtc aactcgccac gcagtggtgt ttgtaataac tccttcagct tttggcccga
     1321 tatcccctgc cccagtaaaa acatcatttt ggtgaccgcc gattcggtgg tgatgtcata
     1381 tccgcccacc acgccgattt tttcaagggc aagaccggtt tcatatttcc ccatctcaac
     1441 agaaccgcct ttacattgtg taacattgta gatggtgatt ccctgcttta tagcttcttg
     1501 cagcgatgca acaaaccatt tatcggtggg tgcattaccg gcaccgtatg tttcaaggat
     1561 aacgcctttt agcccgtcaa tattcaacac cgatttgaca ataatctcag aaatgcccgg
     1621 aaacagtttt agtacggcta catgggtgtc gagcgaataa tgaaccttga gcgatcggaa
     1681 attaggtttc aggatgctgt tgtgattata ccttatatat acgcccactt cggccagcgg
     1741 cggataatta cccgagagga aagcattaaa gttttccgca ttgaatttgt tggtacggtt
     1801 gcctcttaac agtttgtttt caaaataaat agcgacttca ggaactactg gcgtatcata
     1861 tttccggtcg gcagcgattt ccaccgccgt aataaaattg tcgcggccgt cacttcgtac
     1921 tactccgatg ggcaactgcg aaccggtaaa aataaccggc ttgttcaggt tttcaagcat
     1981 aaaactgagc atcgaagcgg aataagccat ggtatcggtt ccgtgcaata cgacgaaccc
     2041 atcgtagttt tcataatttt tctcaattac cgacgccagt tcaatccaga aagccggggt
     2101 tacattggag gaatcgatga gcggggtgaa agtatatgaa tcgatggtat aatcgaaatt
     2161 ccccagggcg ggcatcatct gatataaatt cccgaaatca aaaggtataa gggcgccggt
     2221 ctctttgtcg gtcaccatcc cgatggttcc tccggtattg atgacaagta tggatatgga
     2281 tttcatttca ggggattagg tatttagggg ttaaggagtc tggctaaaag atttcagttg
     2341 attggccgga atttgagcaa ggcacaaaaa tcagagagga aggttaaaga gcttgatagc
     2401 gttccggctg gtgagcgcgg ccacttcttc gactgacgtg cagcacaatt ccgctacctt
     2461 tgccgcgatg aggggtatat acgatggctc gttccgcttg ccgcgataag gaaccggcgg
     2521 aagaaacggc gcatccgttt ccaggacaat atgctgtagc cccaccgatg gaatcacctt
     2581 ttgcagcagc gaatttttaa aggtaactac gccgcctgtt cccagcagga aaccagcttc
     2641 aataacctga cgcgcctgta tctcattacc cgggaaacaa tgaaaaacgc ccttcagatc
     2701 atcggtaagt tcctgcttaa gtatgcgcca aatttcgttg tacgagttcc tgacatgaat
     2761 ggctaccggg agtttgtatc gtttcgccag ctttaactga tgcctgaaaa cctgttcctg
     2821 cagttcaagg tgggttttat cccaatagag gtccatcccg atttctccga cagcaataaa
     2881 ttttccggat tccaggtgct tttcaacagc attcaattct tcttcaatcg tttccggctg
     2941 aaccgatgtc gggtgcaacc cggccataca gaaaaaacgc tgcggatacc tgccggcaag
     3001 cgacagcatt ggtgcccatg aagtactatc aatattgggc aacagcatgt attcaacacc
     3061 caggccgacg gccctctcaa ccgcctgctc acagtcggcg ctaaactcct caagatacag
     3121 gtgggtatgg gtatcgatat acatttgtac gatcggttta taaaaatgca ttattcagtt
     3181 tctcgtaacc gatggtcgtt tcggggccat gtcccggcaa taccctgaaa tcagggggca
     3241 gcgtaaacag ttccctgtag atgctctcgc gcaggagttc aaaattcccg gtcaccagat
     3301 cggtccggcc gatgctgttc tgaaacagta catcgccggt aatcacaaat ttaccggtat
     3361 cgcatatcag gcagatactg ccgtcggcat ggccgggcgt atatgccact cttagttccg
     3421 aatggccgaa gtgaagcata tcgccgtgtt ccagaaatcc agaaggctgt attaccgggg
     3481 atatttcaac gccaaacagc gccccgtatt gcggggccgt ttccagcaca ggtattcctt
     3541 tacggtgagc ataataccca atcctgtatt tgttgcagat ataattattg cccaacacat
     3601 gatccacatg caaatgggta ttaatcagct ttaccggcct gagcccctgt tgactgataa
     3661 acctgtccag atcatctttt tcgttttccg aaacgcagga agtatctatg agcaggcact
     3721 cggatgtttc atcgtacaaa acatagccat ttaccccaaa tgcattgaaa acaaaacgtt
     3781 ttatctgaat cataaggcac tgatcaaaat aattttccag ccgcaaaggt atcaattatg
     3841 catcgccggc caccgttata aaaaagctga aaatattatt gctaccttag catgcctgaa
     3901 attctgtatt aaaatcatgt ttcaaatgaa cgggaaacgg ctggttctct tgattatcgg
     3961 aatattcgcc ggattagtgc taaatccgtt cagcctcagg gcgcagttct ataccggctc
     4021 tcagatgtcg tttggcaaga accgcgtaca ataccttgat tatttctgga catattacaa
     4081 attcgataaa ttcgacgttt acttttatct caacgggaaa gaactggcct tacacacagc
     4141 ccgctatgcc gaaagctgca tacccgggat cgagaagaag cttgaatcca cgattgatac
     4201 caaagaaaag atccagttcc tggtttataa tacccttaac gacctgaggc aaagcaacat
     4261 cggcttgctc agcaatgaac agtacaatac aggcggtgta acgcatatca tcgggcataa
     4321 ggtattcctt tacttcgacg gtgacctgaa tcatctgaat cagcagatca ggtcagggat
     4381 tgccaaaatc atgctcgaaa aaatgatcta cggtgaaacg cttggttcac aaatgaagag
     4441 cagcgccgtg ctaaaccttc ccgattggta cgttgaaggg ctggtatcct atctcggcaa
     4501 tgaatgggat accgagatgg atgataaagt acgggatggg atactctcgg ggcgatttct
     4561 gaaatttaac catcttaaag gtgaagatgc aacaacagta gggcattctg tatggtacta
     4621 catctcgcag aaatacggag agagttttat ccccaacatc gtttatatga ccagggtaag
     4681 ccggggagta gaaagcgggt ttctgtatgt tctcggcatt tcctacaaaa ccctgatgaa
     4741 tgactggaaa gcatggtacg aatcaaaata taaggaggcc ggcgcttaca catcttacgg
     4801 accgtcgcct tccatcagga ttaaatccga cagggtatac gataaagtga agataagccc
     4861 ggatggcgaa aaaatgcttt acgtatgtaa caataacgga ttgtataaga tttttatcta
     4921 tgacctcagc accgggaaga agcatcgaat actcaggaaa ggctaccgtc aggacgaaaa
     4981 aaccgattat acgtacccgc tcctggcctg gcatcccggc agcggggttg ccgctatgat
     5041 caccgaacga aaggggttgc tctggctata cttctatgac ctggaaacca gaaaatggac
     5101 aaaacaaaac atcttcggtt ttcaaaagat tacggacttc agctattcac ccgacggaag
     5161 gatgtttgtg ttttcagccg tccagcgggg agaatcggac attttcctgt acaacatagc
     5221 cgccaattca gccgaacagc tcacccgtga caattttaac gacctgtatc cccgtttttt
     5281 acccgggaca ggcaagataa tcttcagttc caacagaaat gacgacactt tgcgttttga
     5341 aaaggatata ccgttgaaat acaccgggca ggtgaataat gacctcttcc tcatagatgt
     5401 tgcgtcaaaa aaccggaaac tcagccgtgt aaccaatact ccgctcgcaa acgaagtgca
     5461 ggctatgccc tattctcccg gttctttcac ctacctgagc gacgccaatg gcatatataa
     5521 ccgttatgtt gccaggttcg acagcgccat tgcctatgtt gatacagcta cacattaccg
     5581 gtatttcacc cgttcgatcc cagtaacaaa ttttccccga agcatattat ctcaggatgt
     5641 aagcccgatg gccggaaaat cggcagaagt attcaccgaa aaaagcagga gcaggatttg
     5701 cctgtcggat atcgctgctg ccggacctgc cgctgcagta caactggaaa gcacgcctta
     5761 tctccagttt gcaaaacgga aatatgaagc gcagcaggct gaaaaggatg cggctaaaaa
     5821 aggacaacct aaggttaaaa ggaaaaaatt ctcaaacgtt tacgggaaag aggttatacc
     5881 ggctaaagat acagccctta ttgatatcag taattacagg ataaaggaaa taacggaggc
     5941 gcgaagcgat acatccctgg cccgccgtaa ggctgcggcg cacgaaaccg taaaagattc
     6001 cattccccct aaagtccgga attataatgt tgaatatagc atcaataaac taatcagcca
     6061 gttcgatttc tcgttcctca acgccagtta ccagcccttt acgggatatg ccgggccttt
     6121 atatcttcag cccgacgcca cagtcctgtt atcgaccggg gctaccgatc tcctggaaga
     6181 ttaccggatt accgggagta taaaaccaaa tttcaacctc aaaaacaatg agtacctcat
     6241 cagtttttcc aatctcaaat ccaggctcga ccgtgaaata ttcttccacc gcacctctta
     6301 cgatatcact tacaccaatg cactggtaag gcacaggatt catgaaatcc attacatcgt
     6361 cagttatccg tttaccgaaa ccttatgcct gaaaggaaca ggcatgctgc aaagcgacag
     6421 gacgatcgtt ctctcaacgg atcaatacac gcttcaggct gacgatgaaa acaattactg
     6481 ggcttccctg aaaggagaac ttatttttga caacacgcgt gaattgggca tcaacctgta
     6541 tgaaggaatg cgttataaat tctttggaga atattaccag ctcatcgata aaaaaagcac
     6601 caatatagtc attgcaggct gcgacatccg gcattacaca aagattcacc gcacctttat
     6661 ctgggccaac agactggctg ccagcacctc tttcgggggc aacaagctta tgtattacct
     6721 cggcggcgtt gataactgga tttctccggg ttttgactac gacaacagca tcgacaattc
     6781 gcaggattat gctttccaga ccttagctac caatatgcgc gggttcaaac agaatgtgcg
     6841 caatggaaat tcatttgccg tatggaattc agaactccgg ttccctgtct tcaaatactt
     6901 tataaaccgg ccgttgaaat cggatttcct caataacttc cagattgttg gttttgccga
     6961 tttcggttct gcctggaaag gactaaatcc gttttcggag gaaaatacct actttacccg
     7021 gacgatatac caggaacctt tgaaagtgac ggtccaggtc cagaaagatc cgtttgtggc
     7081 cggttatggt tttgggttgc gaaccaggtt attcgggtat ttcatccgtg ccgactgggc
     7141 ctggggcatt gaggatggcg cccgtcagga ccagattttt tacctctcgt tcagtctcga
     7201 tttctaaccc ccgttcattt gtcagtctcc ctcccggcag caatagtcat gaataaagcg
     7261 gggaattaat ttcagaaaaa actgctaaaa atcatcaaat cccatgaaaa tgagcctgca
     7321 agttatattg atattggtgc tcatcggcct tacagccggt atcttcggag gcatggtggg
     7381 cctcggaggc ggcgtcatca tgattcccgc catggttttc ctgctgggaa tgtcacaact
     7441 tgaagcccag ggcaccagcc tggccatcat gctgcccccg atcggaattt ttgccgccat
     7501 caattattac aaagccggtc acattgattg gagatatgca ctcatcattg ccgctgcctt
     7561 tacggtcggt ggatatttcg gatcaaaaat cgccctgaac atcccggccg accttgtcag
     7621 gaaaatattc gccatatccg ttatagccat tgccttaaga atgttcttta aaagttgaaa
     7681 taattcgctt atacctcctc atcatacctg ccgactcatt acatcctcag ctatgcctgc
     7741 cgaaaacatt gataaccgta tcgtttcctt cattcataaa catcacattt tcaccctggc
     7801 gacaattgcc ggtaatcagc cttatacatg cacctgcttc tatgttttca tggattcgct
     7861 gaatcttttt gtctttacct ccgatgaagg cacacgccat gttgcagaaa tgaaaaggca
     7921 aaagcgcgtc gcaggcgctg tagcccttga aaccaaagtc gtggggcgca tccagggcat
     7981 tcagttcact ggcgatagtt ttctcataga gggagatgtt tacgccaaag ccaggaaggc
     8041 ctatctgaag aaatttcctt atgccggatt taaagaactg acattatggg gaatagaacc
     8101 tgattttatt aaaatgaccc acaacatgct gggattcggc accaaactca tctggacaaa
     8161 gaatgacgat ccggcttttt aacattaccc tgctgatggt acaaaacgat agtgccgatc
     8221 atatacttcc gcttaatcgg ccgaaagaaa ataacgctga atagtagcaa cttaattaat
     8281 tttgaatctg attttttttc catattattg taatgcttga ttgacggaca tttcaaaact
     8341 ggctattatc aagggattcg ttacttataa agatagtttt tcactggtta tcaattattt
     8401 aaatgtttct gtttaaattt tgttaataac tgcttttggt gattttttgc accttgtcgt
     8461 aaattggtct gacagtttga gcaacccgga caggtttatc ttctaactgc atttcagtta
     8521 tttatatcct accgctacaa tcttaaatta acccgtcaag cgacttacct atggacgcag
     8581 acctcttttc gagtattgac accagggccg ataaggatac ctccgagact ttctatgatg
     8641 aagtattgaa gaaacgtaat cgccctgata tgaataacga ccctgaaccg gaaccggaac
     8701 aggcgacaca acaaattaaa caacctgaag aagatttgaa gatgcaggaa atgcccctaa
     8761 cccaaccacc aaaaaaattg atcacatacc agcatgaaga agtgatggaa gcagccaccg
     8821 cctatttcaa gggcgacaca ttggcggcca gtgtgtggat gaataaatat gccctgaaag
     8881 acagcgacgg caatatatac gaacgcactc ccgatgacat gcaccatcgc ctggcttcgg
     8941 aagttgcccg catcgaacgg aaatatccca acccgatggg cgaagaggag ttgtttgaac
     9001 ttttccgtga ttttaaatac atcattccgc agggaagccc gatggccggc atcggaaata
     9061 atttccaggt atcgtccctc tccaactgtt tcgtgatcgg aagcgatgaa aaccaggatt
     9121 cctacggagg catcctaaaa gttgaccagg aacaggtaca actgatgaaa cgccgcggag
     9181 gtgtcggaca tgacctttcg catatacgcc cctatggcag cgcggtaaaa aacagcgccc
     9241 tgacttctac cggcattgtt cctttcatgg aacggtattc caattccaca cgcgaagtgg
     9301 cccaggatgg ccgccgcggt gcgctcatgc tctctatttc gatacagcac cccgattccg
     9361 agaatttcat cgacgccaag atggaacagg gaaaagttac cggcgccaat atctcggtga
     9421 agattgacga tgatttcatg aaagccgtga aatccggttc caaatacacg cagaaatacc
     9481 ccataggctc cgacaatcct gccaaaacaa aagagattga agccgggaac ctgtggggaa
     9541 aaatcataca caatgcctgg cagtcggccg aaccgggggt cctcttctgg gataccatca
     9601 tccgcgaatc ggttcctgat tgttatgccg accagggatt taaaaccctt tccaccaatc
     9661 cctgcggcga aattcccctt tgcccttacg acagttgccg cctgctggcg atgaatcttt
     9721 acagttatgt agataatcca ttcacgcccg aagcttcctt caatgcaggg ctctttgcca
     9781 aacacgcccg tcgcgccctc cgcatcatgg atgatatcat tgaccttgaa atggagaaaa
     9841 tcgacggaat cctggccaag atcgatgccg accccgaaga cgaagaaacc aagcgtatcg
     9901 aacggaaact ctgggaaaac atccgttcaa aaactttact gggacgccgt accggcatcg
     9961 gcattaccgc cgaaggcgat atgctggctg cccttggcta tcgttacgga tcggatgaag
    10021 ccaccaattt cagcgtcgaa gttcataaaa cgctggcatt ggaagcctac ggttcatccg
    10081 ttgacctggc tgccgaacgc ggccccttcc ctattttcga catcgagcgt gaaaaaaaca
    10141 atccgtttat caaccgcatc agccggctgg cgccggaggt ttaccaaaaa atgcttaaaa
    10201 acggccgacg gaatatcagt atgctcacca tcgcccccac aggcagcgtt agtatctgca
    10261 gccagacgac ctcgggcatc gaaccggtat tcatggtcag ctacaaacgc cgccgcaaag
    10321 taaatcccaa cgaccgcaac gttaccgtca cttttgtgga tgaagtcggc gattcatggg
    10381 aagaatacaa cgtatttcac ccgaaattcc ttgaatggat gcgcgtaaac ggctacgacg
    10441 ttgaccaggc aaaccatttg cccgaatcgg agatgaatgc cctcatcgaa aaatcgcctt
    10501 actataaagc cacttccaac gatatcgact gggtagccaa ggttaagatg cagggcgcca
    10561 ttcagaagta tgttgaccac agcatcagcg ttacggtgaa cctgccgaag gacgttaccg
    10621 aagaactggt aagcaaggtc tacctcactg cctgggaaag cggctgcaag ggaatgacga
    10681 tctatcgcga tggttcgcgc gccggcgtgc tggtaagcaa cgaaccgaaa aaggaaaaag
    10741 aaagttctgt tgaatttaag gagaccaaag cgccgccccg cccgaagaaa ctcgaagccg
    10801 acgtggtgag attcaccaac gaaagtgaaa gatggatagc cgtggtgggc ctgctcgaca
    10861 ataaacccta tgaaatcttc acgggaaaaa gcgaggactt cttcctgccc gactgggtgg
    10921 aaaaaggctg ggtaatccgc cagaaggaaa ccggaaagtc ggctatgtac aatttccagt
    10981 atctggacag gcaaggttac aaagtaacct atgaagggct gtcgcgcacc ttcgataaag
    11041 agtactggaa ctatgccaag cttatctcgg gaatcctgcg ccacgggatg cccctgcctt
    11101 acgtggtaag cctggtcgaa aacctcactt tcgataagga ctttattact acctggaaaa
    11161 acggggtcgt ccgcacctta tcaaaataca tcaaggaagc gcgccaaacc ggcgaagcct
    11221 gcccgaaatg cggccagacc gatgccctgt accgcaagga aggctgcgta tattgcaaac
    11281 actgtgacta ctcgaagtgc tcataacatc tgctttgatt atgactgccg ccccggttct
    11341 tgcaggttcc ggggctcttt ttttatcctg attcaacatc ag
//
LOCUS       DAOPYV010000085        11325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_6550_length_11325_cov_11.710825, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000085 DAOPYV010000000
VERSION     DAOPYV010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11325
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6550_length_11325_cov_11.710825"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(869..1516)
                     /locus_tag="PKI35_10115"
     CDS             complement(869..1516)
                     /locus_tag="PKI35_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNX44780.1"
                     /translation="MYPEKTIIWDWNGTLLDDLHVCIESINTMLKSRNLPLLTPDSYR
                     DVFTFPVIDYYKAVGFDFTSEPWEEAAMEFMSLYFSKLPACRLASGATETLDFFRNTG
                     YSQAIISAMQHDALVESVTRLGIASYFEYIGGINDHYAGGKTENAVNFFKNRGVSHEN
                     IVMIGDTIHDAEVAGQLHCRCILVAAGHQSRQRLAATGHTVVETLKEVIPLFTGR"
     gene            1654..2130
                     /locus_tag="PKI35_10120"
     CDS             1654..2130
                     /locus_tag="PKI35_10120"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HNX44781.1"
                     /translation="MLQRIQTVYLSIAAVAMAMLFFFPFAVFQSDFVYSRFFIYGVVD
                     MVPGGANSMGIIYALPLILADLGVIIFAVTAIFRYKDRPSQLKLVNAAFILTIILIAG
                     VFFLYGYILEKKFGTPPLWSEGYGVYFPLVALLFLMLAIRSIKRDEKLVRSADRLR"
     gene            complement(2197..>2351)
                     /locus_tag="PKI35_10125"
     CDS             complement(2197..>2351)
                     /locus_tag="PKI35_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HNX44782.1"
                     /translation="FDRKHSLLHINPGAAGNSGWQQVITFVRFVIDNGQIKDLEIFEA
                     ERRQKH"
     assembly_gap    2352..2451
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3263..4354)
                     /gene="corA"
                     /locus_tag="PKI35_10130"
     CDS             complement(3263..4354)
                     /gene="corA"
                     /locus_tag="PKI35_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNX44783.1"
                     /translation="MNTRHIQQHIRTSPKKKYSRKSGQPPGLLLYTGSEPFPTSVGMI
                     QYDEDEFHEKKNIDPSAAFQSLKSDKTNWITVHGFRDTETIETIGKDFGIHPLLMEDI
                     FNVEHLPKIEDMDDYLFITLKSLVWDMPKKQIDAKQVSLLLGKNFLVSFCEDDTPLFE
                     PVVERLKTTKSRARSRKEDYLSYLLLDKIVDNYYVILDQFDDQTDELEDLLLDKPSNE
                     IAQKILALKKQLVILRRTIYPLKEEIRSIAHDESPLINSFTRQYMNDIYDHLIHIIQT
                     IDNFREMISAMMDLLMANNANRMNSIMKTLTLISSVFIPLTFLAGIYGMNFRYMPELE
                     WRYGYFALLGVFSLIAGGMYYYMKKKNWF"
     gene            complement(4437..4841)
                     /gene="mscL"
                     /locus_tag="PKI35_10135"
     CDS             complement(4437..4841)
                     /gene="mscL"
                     /locus_tag="PKI35_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNX44784.1"
                     /translation="MGLIKEFKEFAIKGSVMDLAVAVIIGAAFGKIVTSFVNDVLMPP
                     IGVLLGGVDFKEYKAILVHAVGDTPAVTLNYGMFVQNIIDFLIIAGSIFLLVKALNST
                     KKKSTEAPAAPPAPSKEETLLTEIRDLLKEKK"
     gene            complement(4913..5803)
                     /locus_tag="PKI35_10140"
     CDS             complement(4913..5803)
                     /locus_tag="PKI35_10140"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNX44785.1"
                     /translation="MKKIALTLLAGFMICLCARSAEPDTVRHWKLKAETNIGFSQLAL
                     SNWSAGGENSLATNALINLFADYNKNKFTWNNFAAFGYGVQKQESFSNWRKTDDKIEL
                     TTKAGIYAARQWNYTALLSFKTQFDKGYKYISNDERVLLSQFMAPGYIQLAIGMDYKP
                     ADYFTLLLSPIGSRVTLVDEDSLSKAGAFGVEPGKRSLIQMGGSVTAQFKKDILKNVN
                     LLSKLGLFSNYLKNPENIIVNWENTIFLKVNKHISTTIGTTLIYDDNINSFDKEGNKH
                     GPKTQFKESFSVGFSFSFIK"
     gene            complement(5870..6691)
                     /locus_tag="PKI35_10145"
     CDS             complement(5870..6691)
                     /locus_tag="PKI35_10145"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNX44786.1"
                     /translation="MGIFFQKLPVVILMLTSGISLKVVSQELTHAMPYGSKQELREFI
                     EREMVYPAGELKRGIEGTVVISGLITRNATVANVQVIKSVSPAIDAESLRIFKLLLWE
                     PAKSFGVPADEKVEMEFDFSVKRYEKIVKKRGYNIPALPYPVVDSSGKIYSYRTLEIQ
                     PKPVFIDKDMTMQKFMAKNFTYPENALKQNISGQVSLKFVVEPTGHISNLRVEKHLGA
                     GCSEEALRLVKLMTWTPGIYNGTAVRTMMNLSLLFSLKSGTGYDVQSAQPGFSLQ"
     gene            complement(6765..7520)
                     /locus_tag="PKI35_10150"
     CDS             complement(6765..7520)
                     /locus_tag="PKI35_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX44787.1"
                     /translation="MKRFALTICLLFPFAVLTAETPEAMIVKGNKAYSQGHYEDAIAL
                     YGEVIRMGYESENLFYNLGNAYFKNNELPPAILYYEKALKIDPNDADTRYNLAVANSR
                     IADKIEKIPELFYKRWWTNLKNLFSLDTLSVLIILFVITGLVLAGIYLVSRRMILRRV
                     SFWSAAALLALAFVMLGIASQKNSQLKARKEGIVFTPTVTVKSSPDPSSIDLFVLHEG
                     SKVLVQDSIGGWIEVKIANGSQGWILATDIRKI"
     gene            complement(7544..9442)
                     /locus_tag="PKI35_10155"
     CDS             complement(7544..9442)
                     /locus_tag="PKI35_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNX44788.1"
                     /translation="MNLTKQISRYILQVMLMILLPLVLTGEADAQVHFEASGPSQVGT
                     GQAFRISYSVNKEAKGFIMPEIRNFDILTGPSQGSSSNISFINGKVTQSVSYSYTFVL
                     QAQRAGTFTIPPASVTVDGKKYLSNSLTIQVTDQGGGQRPQARQNARPRSSQPQSSGN
                     ISGNDVFLRAVISKNNLQLGEQAIITYKLYTRIPLSLQGIDKIPSNVGFWSQDLLKNK
                     DKYLQYDEVINGSKYMVAEIRKVAIFPQQTGKLTIDPLQVDVLAQVTVQSKSPFDDDP
                     FFNDPFFRNFFDSSPFSTSTQTVQKALKSNPVTVSISPLPSAGKPVDFTGAVGNFTLK
                     SNIDHNEVKANEAITLRYTITGSGNLSLIEKPNLVFPPDFEVYDPKIVDNINATASGI
                     SGSRTFEYLVIPRVPGNFTIKPVPFTYYDLSRKVYTTLSSQQFNIKVNKGDGTESGLL
                     SSSSNEDIKYIGNDIRYIRHNVTGLHLAGKNFFGNWLFYLLFLLPFVLFGLFLIIWRK
                     QLQLRNNTGLMRNRKANKVATARLKKAKVFLDQKKQEQFYEEVSQALWGYISDKFNIP
                     ISGLSLDTALESLQQRNVDEDISRRFIGNLQHCEFARFAPGDKEAIMQETYREALENI
                     SKTEQQLK"
     gene            complement(9472..10221)
                     /locus_tag="PKI35_10160"
     CDS             complement(9472..10221)
                     /locus_tag="PKI35_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX44789.1"
                     /translation="MIHSPAVILSRKRVNRAPLPGIKRVLAVFAILMLSAGTLSLAQQ
                     PAKFIRKGNSKYNDAKFKDAEINYRKALDKSPKSAKAAYNLGDALYKEGEFDKAASTF
                     STVTGQKTLSDKSKAAAYHNLGNSLLQARKYPESIEAYKNSLRLNPGDEETRYNLAYA
                     LTKLQQQKNNKNNNNGKNKKDQQQQQQQQQQQQQNPENQPQNQPPQNRQMNKQNAERM
                     LQALNNNEKQTLDKVKKNKASAAQVKIEKDW"
     gene            complement(10239..11279)
                     /locus_tag="PKI35_10165"
     CDS             complement(10239..11279)
                     /locus_tag="PKI35_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNX44790.1"
                     /translation="MLRFAHPFALYFLLAIPVFVLYFVLMMRQRKKALKSLGEWTLLQ
                     RLMPEYSEIRSRTKFILLMLAWALLVAALSGPQIGSKLEKIKRRGIDVVFALDVSNSM
                     LAQDITPNRLDRSKQAISRLLDKMDNDRVGIVIFAGKSYVQMPVTSDYAAAKLFLSGI
                     NPGMVPTQGTAIGQAIERSASCFAEGKQSKAIIVITDGENHEDDAVDAAKRAAAQGIK
                     VFTVGMGSPDGAPIPVFNGALQIGYKTDQEGNTIISKLDDRLLNDIALAGNGMYVRAS
                     NSQSGVKEVFDKLNSLEKTEFDSRFFSDYEDRFQFLLVAALIILLAELFLVERKSRLS
                     GKIKLFENNTVK"
BASE COUNT         2651 a         2446 c         2872 g         3256 t
ORIGIN      
        1 tgtgtttttt gtttcatgga attcgtggaa ttcgtggttc gtgggcagtg tttcaatgtt
       61 tcagatctgc ctgccttgcg catggctgtg ggagggggca gggtttcagc gttttgcgtt
      121 ctgtgttttg cgttttgcgt tctgtgtttt gtgttttttg tttcattgaa ttcgaggaat
      181 tcgtggttcg tgggcagcgt ttcagtgttt cgtttggctg ttagcctgcc gtcttacgct
      241 cagcctgccc aggcaggtaa actgttagcc cttggccttt ggcgttttgc gttttgagtt
      301 ttgcgttttg cgtttcgtgg aattcgtggt tcgtgatttg ttagcgtttt gcgttctgtg
      361 ttttgtgttt gtgttttgtg attcattaat agtaattcgt ggcattcgtg attgcattcg
      421 tggaattcgt ggctcattat tcgtgattcg tgattcgtga tttgttagcg ttttgcgttc
      481 tgtgttcggt gttctgtgtt ttgtgttttt tgtttcgtgg aattcgtggt tcgtgattcg
      541 ttagcgtttt gcgttttgtg ttctgtgttt tgtgtttttt gtttcatgga attcgtggtt
      601 cgtgggcagt gtttcagcgt ttcagtgttt caaggtttgg gtttaagcct ttgcgttttg
      661 cgttctgtgt tttgtgtttt ttgtttcgtg gaattcgtgg ttcgtgggca gcgtttcagc
      721 gttttgcgtt ctgtgttctg tgttttgtgt tttttgtttc atggaattcg tggttcgtaa
      781 ttcgttagcg ttttgcgttt tgtgttcggt gttctgtgtt ttgtgttttt tgtttcgtgg
      841 aattcgtggt ttgaaatttg acttttggct atcggccggt aaataacggt attacttctt
      901 tcagcgtttc cacgacggtg tgacctgtgg cggcaagcct ttgccgggac tgatgtccgg
      961 cggcaaccag gatgcagcgg cagtgcagtt gtccggcaac ttcggcatca tggatcgtgt
     1021 cgccgatcat gacaatgttt tcatggctca ctccccggtt tttgaaaaag ttcacggcat
     1081 tttccgtttt tccgcctgca tagtgatcgt tgatgccgcc gatatattca aagtatgatg
     1141 caatgccgag gcgggtcacc gattcaacga gggcgtcgtg ctgcatggct gaaataatgg
     1201 cctgggaata cccggtattt ctgaaaaaat caagggtttc cgtggcccct gacgctagcc
     1261 tgcatgccgg caatttgctg aaatataagc tcataaattc catcgcggcc tcttcccagg
     1321 gctctgatgt aaaatcaaat cctacggctt tgtaataatc aatgacagga aaagtaaaga
     1381 cgtcccgata actgtcgggg gtcagcagcg gaagattcct ggattttaac atcgtattga
     1441 tggattcgat gcaaacgtgc aggtcatcaa gcagcgtccc gttccagtcc cagatgattg
     1501 ttttttcagg atacatgaag tatagaatga taatcagcgt tgtgtatcag tgttccggtc
     1561 acagcggacc tgcaaatgta caaaccgccg tccgatgcaa gtatcagcaa ccgaaaatct
     1621 ttatctttgc gttaaaatca aataacggaa aacatgttac aacgtattca aactgtatat
     1681 ctttcgatag cagccgttgc gatggctatg ctttttttct tcccgttcgc cgtgttccag
     1741 tccgatttcg tctacagccg tttctttatt tacggcgtgg ttgacatggt tcccggcggg
     1801 gcaaattcga tgggaatcat ctatgcgctt ccgctgattc tggccgattt aggggtaatt
     1861 atctttgctg taaccgcgat tttccgttat aaagacaggc cgtcacaact caagctggtg
     1921 aatgccgctt ttatcctcac catcatcctg attgccggcg ttttcttcct ttacggatat
     1981 atcctggaga aaaaattcgg aacgcctccg ttatggtccg aaggctatgg cgtttatttt
     2041 ccccttgttg cgctgctttt cctgatgctc gccatccgct ccatcaaacg cgatgagaaa
     2101 ctggtgcgtt cggccgacag gctccggtaa ttaggttcag caaatctggt ctcctgaacg
     2161 gaaaaggata tatccgtttg tcacgatatt ccggcgttaa tgcttttgac gcctttccgc
     2221 ctcaaatatt tccaggtctt taatctggcc gttgtcaatc acaaacctta cgaaggtaat
     2281 tacctgttgc catcccgaat tgccggcagc gcctggattg atatgcaaca gggaatgttt
     2341 ccggtcgaat annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttttggatg
     2461 aataaagccg tgggtgtcac ttagtaagcc tatgcgtttc attcagtggt tgggattgat
     2521 cgtcggttag gtgatagata tggggcagcc gggagcaggg ggcagggggc agggggcagg
     2581 gagcggggag caaggagcgc ggagaaagga ggacggagga cggagaacgc aaaacggaaa
     2641 acgcaaaacg tagaactcag aactcaaaac tctaacagca aacggcctgc ctgccagggt
     2701 tggctgtgcg taagatggca ggctaaaagc caaagggcaa agggcaaaag gcaaagggca
     2761 aagggaaaag ggaaaaggga aaagggcaaa aggggcaagg ggcaaggggc gcggagaaag
     2821 gagcaggggg cagggagcag ggagcaggga gaacggaaaa cgcaaaacgc aaaacgtaga
     2881 actcagaact ctaacagcaa acggcctgcc tgcctgggtt ggctgtgcgt aagatggcag
     2941 gctaaaagct aaagggcaaa gggcaaaggg caaaggggca aggggcaagg agcaaggagc
     3001 agggagcagg gggcagggag cagggagcaa ggggcgcgga gaaaggagaa cggagaaagg
     3061 aaaacggaaa acgcaaaacg tagaactcag aactcaaaac tctaacggct aatggctaat
     3121 agctaacagc taaaggccaa aggctaaata ccaggcacga cagaacaggt tttggcgcgt
     3181 attgatcggg ggaatattgt attttcctgc tgcccgacct ccggtaatat aacccattgc
     3241 tgaaccttaa acatcctgtg tttcagaacc aattcttctt tttcatatag taatacatcc
     3301 ccccggctat cagggagaaa acgcccagga gggcaaagta accatagcgc cattccagtt
     3361 caggcatgta gcggaaattc atgccataaa tacctgccag aaaagtaagc ggaataaaaa
     3421 cgcttgagat gagcgtgagc gttttcatga tgctgttcat ccggttggcg ttgttggcca
     3481 tcagcaggtc catcatggcc gagatcattt cgcggaaatt gtcgatggtt tgaatgatat
     3541 ggatgaggtg atcataaata tcgttcatgt attgccgggt gaagctgtta atgagcggcg
     3601 actcatcatg ggcaatactg cggatttctt ctttcagcgg gtaaatggtg cggcgaagga
     3661 tcaccaactg tttcttgagg gcgaggattt tctgggcaat ttcattggaa ggcttgtcga
     3721 gcagcaaatc ttccagttca tcggtctggt cgtcgaactg gtcgaggatc acgtaatagt
     3781 tatccacaat tttatcgaga agcaggtacg aaaggtagtc ttccttacgg cttcgcgcac
     3841 gcgatttggt ggtcttcagc ctttcaacaa caggttcgaa aaggggcgtg tcgtcttcac
     3901 aaaaggaaac caggaagttc tttcccagca aaaggctgac ctgtttggcg tcaatttgtt
     3961 tcttcggcat atcccatacc aggcttttca gagtaatgaa gaggtagtcg tccatatctt
     4021 caattttcgg aaggtgttct acgttgaaga tatcttccat gagaagcgga tggatgccaa
     4081 aatctttgcc gatcgtctcg atcgtttccg tgtcgcggaa gccgtgaaca gttatccagt
     4141 tggttttatc acttttgagt gattggaaag cggcagacgg atcgatattt ttcttttcgt
     4201 gaaattcatc ctcatcatat tggatcattc caacggaagt aggaaagggc tcgcttccgg
     4261 tatataatag caatcccggc ggctgccccg atttcctgct gtatttcttt ttaggggaag
     4321 tgcgtatgtg ttgttgtata tgacgtgtat tcatattttc gctgcattaa aaaccggagc
     4381 cggccatgca gccggatccg gaatgattgc cagggtttca gaagcaaggg tattgattat
     4441 ttcttttcct tcagcaggtc cctgatctcg gtaagcagtg tttcttcctt cgagggggcc
     4501 ggaggggcgg ccggcgcttc agtgcttttt ttcttggtcg agttcagagc cttgactaac
     4561 aggaatatag aacctgcaat aatcaggaaa tcgatgatat tctggacaaa cattccataa
     4621 ttgagcgtaa cggcaggagt atcccccaca gcatgaacca gaattgcttt gtactcttta
     4681 aaatcaacgc ctcccagcag tactccgatc gggggcatca gaacgtcgtt tacgaaagag
     4741 gtaacgatct tgccgaaggc ggcgccaatg atcacggcaa ctgccaggtc cataacgctg
     4801 cctttgatgg caaattcttt aaattcctta atgagtccca tgtttattga ggtttggttc
     4861 gtaaattgga ttaattggag atgaaattac gccaataatc aaagcatgtc aactatttaa
     4921 tgaaactaaa tgaaaatccg acagagaagg attctttaaa ctgtgttttc gggccgtgtt
     4981 tattcccttc tttatcaaag gaattgatgt tgtcatcgta gattaacgtc gtacctattg
     5041 ttgtcgaaat gtgcttgttc acttttaaaa atatcgtatt ttcccagttc actatgatgt
     5101 tttcgggatt tttcagataa ttggagaata gaccgagttt cgacaagagg ttgacatttt
     5161 taaggatatc cttcttgaat tgggctgtga ccgatccccc catctggata agcgatcgct
     5221 tacccggctc aaccccgaaa gcgcctgctt tggaaaggga atcctcgtcg accagcgtga
     5281 cgcgggaacc gataggcgag aggagcagtg taaaataatc ggcaggctta taatccatcc
     5341 cgatagccaa ctgaatatac ccgggggcca tgaactgtga aagcagcact ctttcgtcgt
     5401 tgctgatata cttgtagcct ttatcaaact gcgtcttgaa actcagcaaa gcggtataat
     5461 tccactgccg ggcggcatat atccctgctt ttgtcgtaag ttcaatttta tcatcggttt
     5521 tcctccagtt cgagaaactt tcctgttttt gaacgccgta accgaaagcg gcaaagttgt
     5581 tccaggtgaa tttgtttttg ttataatcgg caaaaaggtt aatcaaagcg ttggtagcca
     5641 gtgaattttc acctcctgcc gaccagttgg aaagcgccag ttgcgaaaag cctatattgg
     5701 tttcagcctt tagtttccaa tgtcgtaccg tatcgggctc agcgctgcgg gcacaaaggc
     5761 aaatcatgaa tcctgccagc aacgtcaggg caatcttctt cataaaaact catttattaa
     5821 aatggactgc aaaaatatac aagaaatcta tatgcctgaa aatcgagcgt tattgaagtg
     5881 aaaatcccgg ttgcgccgat tgcacatcat acccggttcc gcttttcagg ctgaataata
     5941 gcgagagatt catcattgta cgaacggcgg ttccgttgta tatgcccggt gtccaggtca
     6001 tcagttttac caggcgcagg gcctcttcgg aacaaccggc gccaaggtgt ttttcaaccc
     6061 tcaggttgga aatatgcccg gttggctcta cgacgaattt cagggatact tgtccgctga
     6121 tattttgttt cagggcattt tcgggatacg tgaaattttt agccataaac ttctgcatcg
     6181 tcatgtcttt atcgatgaaa accggtttgg gctgtatctc cagcgtcctg taggaataga
     6241 tctttcccga actgtcgacg accgggtacg ggagcgccgg aatgttgtat ccccttttct
     6301 taactatttt ttcgtaacgc ttcaccgaaa agtcaaactc catctccacc ttttcgtcag
     6361 cgggaactcc gaaagatttt gcgggttccc acaacaggag cttgaaaatt ctaagggatt
     6421 cggcatcaat ggcaggggaa acgcttttaa tgacctgcac attggcgacg gtggcatttc
     6481 gcgttataag gcccgaaata acgacggtac cttcaattcc gcgtttcagc tcgccggcag
     6541 gataaaccat ttccctttca ataaattccc tgagctcctg tttgcttccg taaggcatgg
     6601 cgtgcgtcaa ttcctgcgac acgactttga ggctgattcc tgacgtcagc atcaggataa
     6661 cgaccggcag tttttgaaaa aaaatgccca taaaaaagct gttagatgtt aacagctaag
     6721 ataagaatga aaactggctt attcaacctg aaatgaccgg aaaattaaat tttcctgata
     6781 tcagtagcca gaatccagcc ctgactgcca ttggcgattt ttacttcgat ccatcctcct
     6841 atggaatcct gaaccaacac tttagagcct tcgtgcaaaa caaacagatc gatgctcgaa
     6901 ggatcgggcg agcttttaac agtaaccgtc ggggtgaaaa caatgccttc tttccgggct
     6961 ttcaactggc tgtttttttg tgaagcgata cccagcataa caaatgccag cgccagcaat
     7021 gccgcagcgc tccagaaaga tacccggcgc aggatcatgc gcctcgaaac aagatatatg
     7081 ccggccagga cgaggcctgt aattacaaaa agaataatga ggactgaaag ggtatcgagt
     7141 gaaaacaggt tcttcagatt ggtccaccag cgcttataaa atagttccgg tattttttct
     7201 attttatcgg caatacggct gttggcgacg gccaggttgt accgggtgtc ggcgtcgttc
     7261 ggatcgatct tcagggcctt ttcataatac aggatggccg gtggcagttc gttgttttta
     7321 aaatacgcgt tgccgaggtt gtaaaataaa ttttccgatt cgtaccccat cctgatcacc
     7381 tctccgtaaa gggctattgc atcttcataa tgtccctggc tgtatgcttt gttccctttt
     7441 acaatcatgg cttcaggcgt ttcagccgtc aggacggcaa acgggaataa caggcaaatc
     7501 gtaagggcaa accttttcat agcgggaaag gtatatgatt gagttatttt aattgttgtt
     7561 cggttttgga aatattctct agggcttccc ggtatgtttc ctgcatgatg gcttccttgt
     7621 cgccgggcgc aaaacgggca aattcgcagt gctgaagatt cccgataaac cggcggctga
     7681 tgtcctcgtc aacattccgc tgctgaagcg actccagggc cgtatcgagt gaaagcccgg
     7741 aaatgggaat attgaatttg tcgctgatat atccccataa cgcctgcgat acctcttcgt
     7801 aaaactgctc ctgttttttc tggtcaagga aaacctttgc cttcttcagt cgtgccgtag
     7861 ccaccttatt ggccttacgg ttacgcatga gtccggtatt atttcgcaac tgaagctgct
     7921 tgcgccagat aatcaggaac aacccgaata aaacgaatgg caggaggaat aaaaggtaaa
     7981 agagccaatt cccgaagaaa ttcttaccgg caagatgcaa tccggtcaca ttgtgccgga
     8041 tgtagcggat atcgtttccg atgtacttga tgtcttcgtt ggatgaggac gacagcaaac
     8101 cggattccgt tccgtcccct ttgttcactt ttatgttgaa ttgctgcgac gaaagggtgg
     8161 tgtacacttt ccgcgaaagg tcgtaatagg tgaaagggac cggcttgatg gtgaaatttc
     8221 ccggaacacg cggaatcaca aggtattcaa atgtccgtga acccgagatg cctgaagcgg
     8281 ttgcattgat attatcaaca atcttcggat cgtacacttc aaaatcggga ggaaagacga
     8341 gattgggctt ttcgatgagg ctgaggttgc cgctgcctgt gatggtgtac cgcaatgtga
     8401 tggcttcatt ggctttgacc tcgttatgat cgatgtttga cttcagggta aaattaccta
     8461 cggcgccggt aaaatcaaca ggcttgccgg ccgatggcaa cgggcttata cttactgtaa
     8521 ccggattcga tttcagcgct ttctgtacgg tctgtgttga ggtgctgaaa ggggaactgt
     8581 cgaaaaaatt cctgaagaac ggatcgttga aaaacggatc gtcgtcgaaa ggcgatttcg
     8641 actgaacggt aacctgcgcc aatacgtcga cctgcagcgg atcaatggtg agtttgccgg
     8701 tctgctgcgg gaaaatagca acttttctga tctcagccac catgtatttc gagccgttga
     8761 tgacttcgtc atattgcagg tacttgtcct tgtttttcaa aagatcctgc gaccaaaacc
     8821 ctacattcga agggatctta tcgatgccct gcaacgagag cggtatgcgg gtgtacagct
     8881 tataggtaat gatggcctgt tcgccaagct gaaggttatt tttggatatg accgctctga
     8941 gaaaaacgtc atttcccgaa atgttcccgg aactttgcgg ctggctgctt cgcgggcgag
     9001 cgttctgacg ggcctgcgga cgttgcccgc cgccctggtc agtcacctga atggtcagtg
     9061 agttcgaaag gtatttcttg ccgtcaacgg tcaccgatgc gggaggaatg gtaaaggtgc
     9121 cggccctttg ggcctgtaat acaaacgtgt aggaataact taccgactgg gtaacttttc
     9181 cattgataaa ggaaatattg ctgcttgaac cctgcgacgg gcctgtcaat atgtcgaaat
     9241 tgcgaatctc aggcatgatg aagccttttg cttccttgtt gacggaatag gagatcctga
     9301 atgcctgtcc ggtacctacc tgcgacggcc cgctggcttc aaagtgcacc tgtgcatcgg
     9361 cttcacccgt taatacaaga ggtaacagga tcatcagcat gacctgcagg atgtagcgag
     9421 atatttgttt cgtcaggttc atagtatcgt tggcaggttg aaacaaagct tttaccagtc
     9481 tttttcaatt tttacctgtg ccgcactggc tttgttcttc tttaccttat caagggtttg
     9541 cttttcgttg ttgttgagcg cctgcagcat gcgttccgca ttttgcttgt tcatttgcct
     9601 gttctgcggc ggctggttct gtggctggtt ttcagggttt tgttgctgtt gttgctgttg
     9661 ctgttgctgt tgctgctggt cttttttatt cttgccgttg ttattgttct tgttattctt
     9721 ctgctgctgt aatttggtaa gggcataggc caggttgtaa cgggtttctt catcgcccgg
     9781 attaagcctt aatgaattct tgtatgcttc gatgctctca gggtatttcc tggcctgtaa
     9841 aagcgagttt cccaggttgt ggtaagcggc ggctttgctt ttatcgctca acgtcttctg
     9901 gccggtaacg gtggagaaag tagatgccgc cttatcaaat tctccttctt tgtataaagc
     9961 atctcccagg ttgtatgcgg ctttcgccga ttttggcgat ttatccagcg ctttccggta
    10021 attgatttcg gcgtctttaa atttggcgtc gttatatttt gaattgcctt tgcggataaa
    10081 ttttgccggc tgttgcgcca ggctaagggt gcctgccgac agcatcagta ttgcaaacac
    10141 ggccaaaacc ctttttatac cggggagcgg cgccctgttt actctttttc gggataatat
    10201 tacggctggt gaatgtatca ttgattttgc accggatatc atttaacggt attgttttca
    10261 aaaagtttta ttttgcccga cagcctgctt ttcctttcaa cgaggaacaa ctcggcaagc
    10321 aggatgataa gggcggctac gagcaggaac tggaaccggt cttcataatc ggagaagaac
    10381 cgggaatcga attctgtctt ttcgaggctg tttagtttat cgaaaacctc cttaactccc
    10441 gactggctgt tgctggccct tacatacatt ccgttaccgg ccagggcaat atcgttcagg
    10501 agcctgtcgt cgagtttcga aatgatggta ttgccttcct gatcggtttt ataacctatt
    10561 tgtaatgctc cgttaaagac agggatcggt gcgccgtcgg gagatcccat gcctacggtg
    10621 aacaccttga taccctgcgc tgcggccctt tttgcggcgt cgacggcgtc atcttcatgg
    10681 ttttcgccat cggtaatcac gatgatggcc ttgctttgct tgccttcggc aaaacaggat
    10741 gcgcttcttt caatggcttg cccgatagct gttccctgtg ttgggaccat tccgggattt
    10801 attcccgaaa ggaataattt tgcggcggca taatcggagg taaccggcat ctggacatac
    10861 gatttccctg caaagatcac gatgcctaca cggtcattgt ccattttgtc gagaaggcgc
    10921 gaaatggcct gtttcgaacg atcgagcctg ttgggggtga tgtcctgcgc cagcattgaa
    10981 tttgaaacat caagggcaaa aacaacgtcg atgcctctgc gtttaatttt ttccagtttc
    11041 gatccgattt gcggaccgga aagagctgcg accagcaatg cccaggccag catcagcagg
    11101 atgaactttg tcctgctccg gatttcggaa tattcaggca tgagccgttg cagaagtgtc
    11161 cattcaccta aagatttcaa cgcttttttc cgctgacgca tcatcaggac gaaatacaac
    11221 acaaacaccg gtatcgccag caggaaataa agtgcaaacg gatgggcaaa acgtagcatt
    11281 tgtattgaaa tttagttgtt tattattatt tggctgttag ctgtt
//
LOCUS       DAOPYV010000086        11098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_6750_length_11098_cov_10.881101, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000086 DAOPYV010000000
VERSION     DAOPYV010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11098
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6750_length_11098_cov_10.881101"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..700
                     /locus_tag="PKI35_10170"
     CDS             56..700
                     /locus_tag="PKI35_10170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX44791.1"
                     /translation="MKNQAELFWDKHAKVFDDNQKPFETASLEILTRTKEYLDAKDNV
                     LDFGCATGAKTLELSGGVKNIRGLDFSAEMIGEAIKKKNKANAANVTFSQGTIYADDL
                     GKASFDKILAFSIIHLLDDSEKAVQRIHELLKPGGLFIAETACFKNRMNFRTRAGFAF
                     TRFMIRLGIFPLHLNMYTSADVEKLISSHDFNILKAEVFFFNGMTISFIVAEKH"
     gene            967..1176
                     /locus_tag="PKI35_10175"
     CDS             967..1176
                     /locus_tag="PKI35_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX44792.1"
                     /translation="MDLASFVKKKRKSVKLTQPELAEKAGVGLRFIRELEQGKQSLRL
                     DKVNQVLQLFGYQVGAVPAKPGADS"
     gene            1230..1562
                     /locus_tag="PKI35_10180"
     CDS             1230..1562
                     /locus_tag="PKI35_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HNX44793.1"
                     /translation="MRKAAIKIEGQIAGRLTQDEQGYHFVYDNAYATRPDARPVSLTL
                     PVREAPYTSNVLFPFFDGLIPEGWLLDIAEKNWKLNPRDRMGLLLACCKDCIGAVSVE
                     AITEEDAP"
     gene            1559..2500
                     /locus_tag="PKI35_10185"
     CDS             1559..2500
                     /locus_tag="PKI35_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNX44794.1"
                     /translation="MSRCLFCYQKLADNEKDFHISCSKKMFGQPVPPLLPYSEEDLEP
                     LALEVIQSQTAVTGVQAKLSLHITGNHKAGTEKRFTIVGLWGGYILKPPIALYPQLPE
                     VEDVTMHLAQSAKIKTAPHSLIRLTSGNLAYITKRIDRTNKGKLAMEDMCQLTERLTE
                     DKYHGSYEQIGKVIKKYSATPGLDVVNFFELVLFSFLTGNADMHLKNFSLLEQPESGM
                     TLSPAYDLVNTTLVNPADDEELALNLNGRKKKIKKQDFVAAMNTLQVEEKQQQNIFNK
                     MAGAMPKWFELIDNSFMSDVFKNRYKALITEKAKVIL"
     gene            2775..3941
                     /locus_tag="PKI35_10190"
     CDS             2775..3941
                     /locus_tag="PKI35_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNX44795.1"
                     /translation="MKGNSLNRRKFFTVVGAGSLVLAAKPLSSIASSFVPVDDKPATN
                     IADAAKTPRNELSMPGKFPGKVIHVKNPKAVVNGEPVELEAYLMIKKAMLELTGQKNL
                     KKAWRMFVKPGERIGLKVNPVAGKLLSTSHAVTKSVIRQLLESGIRKEDIMIWDRRDM
                     ELKDVGYTAENYPGIKIAGTELQDDQGAFYDKEGKLYGENNIDKEWFYWADVEGEYDS
                     ETMPYMINGGKYSYFTKIVTRDLDKIINIPILKNAGNSITNAMKNLAFGCISNTGRLH
                     AQLWNETCAEVCAFAPIRDKVVLNICDGLRGCFNGGPAANPQFICDFNSILVATDAVA
                     LDRIGYDIVVEKRIAKGIQKTATPEKLKFLAMSSDLGLGISDKDRIDLKVFDLE"
     gene            3984..4811
                     /locus_tag="PKI35_10195"
     CDS             3984..4811
                     /locus_tag="PKI35_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44796.1"
                     /translation="MTRYCRIFFLLPFISLTGFSQEPVTYPAIGTSDLPDAVFEAPKT
                     YTGASLYGYMDGGADLYLEYGLAGARIDEVTMTGRKYTIETYRMNGPEGAFGIYSVSR
                     YQCKSAPPLAPFTCLTPYQLQIVVGPFYINIINSSGASADSAASLKIGEAIVRKVTDK
                     PADISRFMPEIPMETINREAILVKGELGLRNGAPDLADYFEGMSGYCAVILQQTEQVL
                     ISVRFKDKEDTEAFTALHSPGQRSATASAADAGEETITRISENQLLIRIQNSGNREE"
     gene            complement(4925..5443)
                     /locus_tag="PKI35_10200"
     CDS             complement(4925..5443)
                     /locus_tag="PKI35_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44797.1"
                     /translation="MTTKGYFIAILTALLVSAGCTIPRHVPRPNEADEYPYGSEIHVE
                     LAAWQNGRLYIKGELLAADSACMMILPFDKKMKEAVIVKFSSVKDYTLYYAKPKNYSP
                     AILLFLLSTFSHGGFMVLSVPVNIIVTTATTCKAAKVFTYRKRDLTPDQLRMFSRYPQ
                     YKPEDLPVTGYR"
     gene            complement(5433..7043)
                     /locus_tag="PKI35_10205"
     CDS             complement(5433..7043)
                     /locus_tag="PKI35_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44798.1"
                     /translation="MNRKFLNTLKRPFWLSVILAATFSCATPPYYFRSNYNDINRLIH
                     EEKNLQTRPFLKAHLKNGGVVILRDTWNVDSTQRFISGNGTSYDKRRIKVSEGPMDIP
                     IDSVAIFETNKKLAYDESGRIAGLSILAGLDVALGVFCLTVPKACFGSCPTFYINPGD
                     NFHHADAEGFSHAFFPSREYSDLDALPRTKTREKEFRITMKNEALETHCVNDISLYAW
                     PVKAQEKVCQSGNNDFYLCTNLYPLTEATGEEGDITNLLRNNDLVERFSLSDKDNLKS
                     KEEIIITFDSLPEIMDPGLVIHFRQTLMTTYFIYNCMGYMGNYVGDAYAMIESGKMKM
                     RDNKRFYEELGGIDVYSLDENSGRWIFRGEVYETGPIAVNKQMVPLKIPDSLNRLKLK
                     LVLNKGLWRLDYLALTEIKEKIDPLVIGPAGVLNKGKEDSAALALIRNPEEYLVSMPG
                     DEYTFRFQLPDERDYELFLYSKGYYLEWMRNSWLKDEDLLKLRQMTYHPDKYFRKQAA
                     EYKSYETLMEQIFWNSKIEAETFSYHDN"
     gene            complement(7389..7604)
                     /locus_tag="PKI35_10210"
     CDS             complement(7389..7604)
                     /locus_tag="PKI35_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44799.1"
                     /translation="MKKTVRLPAFNTENSKKVIPKDLITGCGIRDPEPAANMQAGFQA
                     SRRNWPVNLVAAGTYYLINRGRLQGTL"
     gene            7889..8431
                     /locus_tag="PKI35_10215"
     CDS             7889..8431
                     /locus_tag="PKI35_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44800.1"
                     /translation="MKKNLVIYYSNSGSNKYLANRIAGSLACDITEIRPRLNAFPLLL
                     LFSSLKRSFGIKALKPNPGEYERIILCGPVWMGKLISPLRDFLFKYRKSIDSLYFASC
                     CASSDIAKDEKYGHGLVFNMVREIMGQKCIHCEAFPVGLVVPEDKKEDGNTIMKTRLS
                     DDNFNGEILLRFEKFIQLLA"
     gene            8762..9229
                     /locus_tag="PKI35_10220"
     CDS             8762..9229
                     /locus_tag="PKI35_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44801.1"
                     /translation="MKRTWFVTVLIALMIAVISPVKLLGQDTKPDKAREKSKSENVNK
                     PQKTEDNVTPGNIPAGQEGKAVTGEKEKGYQKTGDTGKKEGWNQEKKTGADKAGIAKD
                     KKTREKEMKKARKMREEKARAAKKEREKMMKEREKERAKAAKEKKEARKQHSK"
     gene            9672..>11098
                     /locus_tag="PKI35_10225"
     CDS             9672..>11098
                     /locus_tag="PKI35_10225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44802.1"
                     /translation="MKKSISLLALLLFLSASLFAQKTEYIRKKNEDLFLLKNRLEKPD
                     KNAAKEKRIPHFRDMITKKQNHSLKSTDVIKQRLDSITGGYWDDYSSQWLPESKDEFT
                     YDDNGNNTKLIECEWEPDDNRWIPYDKQEYSYDAEGRIIEYLDYEWDDATSQWESFWK
                     SSFTYDVNGNMIQLVDYYWSDYLNNWTFDTKTDMTYDAAGNLVQDIEYFWDEDITQWI
                     NSTKGEYTYNTGNNIILEIFCDWDENSSAWVNFCKTEYSYDTGDKLILILDYQWNTTG
                     WIASNKMDYTWDLNGHVVQEVFSSWDESSSLWVYAFKSEHIYDSDGNLTQELSYLWEQ
                     SVSQWVAESKDEYEYNNSFSFSNLLLPFLFLYDGEDVLAYFNHMLTHINNYVWEDNQW
                     IFNYKTVLDYSPQEVLSINSISAGSNQVSPNPVSDYLTVTLDKNYDKARFELFDMQGK
                     KVLSENVMNHEKVSLQGFSAGIYYYV"
BASE COUNT         3185 a         2556 c         2497 g         2860 t
ORIGIN      
        1 gccttttgtt aaggcattga atgccatttt ccggtaaccc atacaaatac aaatcatgaa
       61 gaatcaggca gaactatttt gggataaaca tgcaaaagta tttgacgata accagaagcc
      121 attcgaaacc gcatcactgg aaatacttac cagaaccaaa gaatatcttg atgcaaagga
      181 caatgtgtta gactttggat gtgccaccgg ggctaaaacc ttagaactgt ccggcggagt
      241 aaaaaatatc cgcggcctcg atttttctgc tgagatgatc ggcgaggcca taaaaaagaa
      301 aaataaggca aatgccgcga atgtcacttt ttcgcagggg accatttatg ccgatgatct
      361 gggaaaagct tccttcgata aaatacttgc cttttcaatc attcatttgc tggacgacag
      421 cgaaaaagct gttcaaagga tccatgaatt gctgaagccc ggcggattgt tcattgctga
      481 aacagcctgt ttcaaaaaca gaatgaattt caggaccagg gcaggatttg cttttacccg
      541 ctttatgata cggcttggaa tttttccttt gcatctgaat atgtatacgt ctgccgacgt
      601 tgagaaatta ataagcagtc atgatttcaa tattcttaaa gcagaagtat tcttttttaa
      661 cgggatgacc atcagcttca ttgtagctga gaaacactag tgagggatac tgattatggg
      721 taccggggaa ggagcagatc cctgccggtg cggatgtgta atcggtaccc gcttttatcc
      781 tcccactccc cgctggcgca cacgcttgaa gcatgtgccg ccatagtacc cgatcggggt
      841 taaacgttcc tcgctttatc atttatacac ccgaaaaggt atattaattg cttattttaa
      901 taaaatacac ccgaaagggt gcaaaactga aaattagtat tatatttgca cccaaaaggg
      961 tataaaatgg accttgcaag ctttgtaaaa aagaaacgga aatcggtaaa gctcactcag
     1021 cccgaactgg ctgaaaaggc cggcgtgggt ttgcgtttca tccgggaact ggaacaaggc
     1081 aaacaaagcc tgcgcctgga taaagtaaac caggtgctgc aactattcgg ttaccaggta
     1141 ggagccgtcc cggcaaaacc cggcgccgat tcctgattct ttcgttcaag ttccagggtt
     1201 cacggattcc agattccaga ttcaccaaag tgcgaaaagc tgccataaaa atagaaggcc
     1261 aaatagccgg ccggctcacc caggatgagc aaggctatca ttttgtgtat gataatgcgt
     1321 atgcaacccg acccgatgcc cgtcctgtta gtctcacctt gccggtgcgt gaagcgcctt
     1381 atacatcgaa tgtgttgttt ccgttttttg acggcctgat acccgaaggc tggttgctcg
     1441 atatagccga aaagaactgg aaattaaatc cccgggaccg tatgggatta ttgctggctt
     1501 gttgtaaaga ttgcattggt gcagttagtg tggaagcaat aacagaggag gatgcgccat
     1561 gagccggtgt ttattttgct accagaaatt ggctgataat gagaaggatt ttcatatatc
     1621 ctgctcaaag aaaatgtttg gtcagcccgt gcctccttta ctgccttatt cagaagaaga
     1681 cctggagcca cttgctctag aagtgataca aagccagaca gcagtcaccg gcgtgcaagc
     1741 caaattatcc ctgcatatta caggcaacca taaagcaggc actgaaaaac gatttaccat
     1801 tgttggcttg tggggaggtt acatattgaa accacccatt gcattatatc cgcaactgcc
     1861 ggaagtggaa gatgtcacta tgcatctggc acaatcagct aaaataaaaa cagcgccaca
     1921 tagtttgata cgcctgacgt cgggcaatct tgcttacata accaaacgaa ttgaccgcac
     1981 aaataaagga aagctggcca tggaagacat gtgccaactc accgaacggc ttaccgaaga
     2041 caagtatcac ggcagttacg aacagattgg caaagtcatt aaaaaatatt cggccacccc
     2101 tggtttggat gtagtaaact tttttgaatt ggtactcttc tcattcctca ccggcaatgc
     2161 cgatatgcat ttgaagaatt tctccctgct ggagcaaccg gaatctggca tgacgttgtc
     2221 accggcgtat gatttggtga atacaacttt ggtaaaccct gccgatgacg aggaactggc
     2281 gttgaatcta aacggcagaa aaaagaagat aaagaagcag gattttgtgg cagccatgaa
     2341 caccttgcag gtggaagaaa agcagcaaca aaacatcttt aacaagatgg ccggtgcaat
     2401 gcctaaatgg ttcgaactga ttgataacag ctttatgagc gatgttttca aaaacagata
     2461 caaagccctg ataaccgaaa aagctaaagt tattttataa ctccgggctt tcgccggcgc
     2521 gcgtatcctt aagcgtgcct tgcctctgtc attgaaaaaa tttaaataca acttcgttct
     2581 ccgggcttcc atctcatgcc gccggcgccc tctgtttcgc ctgttcataa gaatttgccg
     2641 cctcccgggt cagtatacaa atcatattca taaatttggc atgacagaaa accatctgat
     2701 accaaccgga tttataaatc aaccaaaaca aatctgttat cacgccgaaa gccatcggaa
     2761 taacacatcc ccctatgaaa ggaaattcac tcaaccgcag gaagttcttt accgtcgtcg
     2821 gagccgggag tctggtcctg gccgccaagc ccttatcatc cattgcctca tcgttcgtcc
     2881 cggtcgatga caaaccggca accaacattg ccgatgcagc caaaacaccg agaaatgagc
     2941 tgtcgatgcc gggaaaattt ccgggaaagg taattcatgt taaaaatccg aaggccgtcg
     3001 tcaatggcga accggttgag ttggaggcct atctgatgat aaagaaagcc atgctggaac
     3061 tcaccggaca gaaaaacctg aagaaggcgt ggagaatgtt tgtgaagccg ggcgaacgca
     3121 tcggtctgaa agtgaaccct gtagccggga aactcctttc gaccagccat gccgtcacca
     3181 aatcggtcat caggcagctt ctggaaagcg gcatccgtaa ggaagacatc atgatatggg
     3241 accggaggga tatggaactg aaagatgtcg ggtatacggc tgagaactat cccggaataa
     3301 aaatagccgg aaccgaattg caggatgacc agggggcttt ctatgacaag gaaggaaagc
     3361 tttatggcga gaacaacatt gataaagagt ggttctactg ggccgatgtg gagggggaat
     3421 acgacagcga gacgatgcct tacatgatca atggcggcaa atactcttac tttacaaaaa
     3481 tcgtcacccg cgacctcgat aagatcatca acattccgat tctcaaaaat gcgggcaatt
     3541 ccattaccaa tgccatgaaa aacctggcat tcggttgcat ttccaatacc ggcaggcttc
     3601 atgcgcagct ctggaacgaa acctgcgccg aagtatgcgc cttcgctccc atacgcgaca
     3661 aagtggttct caacatctgc gacgggctcc ggggttgctt caatggcggt ccggccgcca
     3721 atccccagtt catctgtgat tttaattcca tcctcgttgc caccgacgcg gttgccctgg
     3781 acaggatcgg ctatgacatt gttgttgaaa aacgcattgc caaaggcatt cagaaaaccg
     3841 ccacaccgga aaagttaaaa ttcctggcta tgtcgtccga tctcggatta ggcatatccg
     3901 acaaagacag gatcgacctg aaggtattcg accttgaata aaatagattc ctgcccgtaa
     3961 ttgacttaaa ctgacaactg ttcatgacaa gatattgccg gattttcttc ttactgccct
     4021 tcatttccct gaccggtttt tcgcaggaac ctgtaactta tcctgctatc ggcacaagcg
     4081 atctgccgga tgctgttttt gaagccccca aaacctatac gggagcttcg ctttacggct
     4141 atatggacgg aggcgccgac ctttaccttg agtatggtct ggccggagcc cggattgatg
     4201 aagtaaccat gacgggaaga aagtatacca ttgaaacata ccggatgaac ggcccggaag
     4261 gcgccttcgg gatatattcc gtttcacggt accagtgcaa aagcgccccg cctttggcgc
     4321 cgtttacctg ccttacgcca taccagttgc aaatagtggt cggaccgttc tacatcaata
     4381 tcatcaacag ttccggcgcc tccgccgaca gcgctgcttc cctgaaaatc ggcgaagcga
     4441 tcgtaaggaa agtaacggat aagccggctg atatcagccg ttttatgccg gaaattccga
     4501 tggaaactat aaaccgggaa gcaatcctgg taaaagggga gctggggctc aggaacggcg
     4561 cccccgacct ggctgactat ttcgagggga tgtccggata ttgtgccgtt attcttcagc
     4621 agacggaaca ggtccttatt tcggtccggt tcaaggacaa agaggatacg gaggcattca
     4681 cagcccttca ttcccctggt cagagaagcg cgacggcctc agccgctgat gcgggggaag
     4741 aaaccatcac cagaatttcg gagaatcagt tgctgatccg catccaaaac agcgggaacc
     4801 gggaagaata aagccggcag tgtttctgat tgtttcagcc gttttaaaac cagaaattga
     4861 atgccggtgt gagtgaacat tctccggtaa gatatccgct gatccatccg gtcgtttaaa
     4921 attgttaccg gtatcccgtc accggcaggt cctccggttt atactgggga taccggctaa
     4981 acatacgcaa ctgatccgga gtaagatccc ttttcctgta tgtaaaaact tttgctgctt
     5041 tacaggtggt tgccgtagtt acgatgatat tgacaggaac tgataatacc ataaatcccc
     5101 cgtgcgaaaa tgttgataat agaaacaaga gaatggccgg cgaataattt ttcggtttgg
     5161 catagtacaa tgtataatcc tttacagagc tgaattttac aattaccgcc tccttcattt
     5221 tcttgtcaaa gggtaaaatc atcatacagg cagaatcagc ggccagcagt tctcctttga
     5281 tgtatagacg gccgttctgc caggccgcca attcaacatg aatctccgag ccatacggat
     5341 actcatccgc ttcgttcggc cgcgggacat gcctgggaat tgtgcagcct gccgacacca
     5401 gcaatgccgt gagtattgcg atgaagtaac ctttagttgt catggtacga aaatgtttcg
     5461 gcctcaattt tcgagttcca gaatatttgt tccatcaatg tttcatagga tttatattct
     5521 gcggcctgct ttctgaaata cttgtcagga tggtacgtca tctgccgcag tttcaaaagg
     5581 tcttcgtctt tcagccaact gttacgcatc cattccaggt aatatccttt tgagtaaaga
     5641 aacagttcat aatccctttc atcaggaagc tgaaagcgaa acgtatactc gtcgcccggc
     5701 atcgagacca ggtactcttc agggtttctt atcaatgcca gggctgccga atcctccttc
     5761 cccttattca ggaccccggc cggaccgata accagcgggt cgattttttc ttttatttcg
     5821 gtcagcgcca ggtaatccag ccgccacaat cctttgttaa ggaccagttt cagctttaac
     5881 cggtttaagc tgtccggtat ttttaacggc accatctgtt tattcacggc aatgggtccc
     5941 gtttcgtaca cttcgccccg gaatatccat cggccgctgt tttcatccag ggaatataca
     6001 tcgatgccgc ctaattcttc ataaaaccgt ttgttgtctc tcatcttcat ttttcctgat
     6061 tctatcatcg cataagcatc cccgacgtaa ttccccatgt aacccataca attgtaaata
     6121 aaataggttg tcatcagggt ttgcctgaaa tgaatcacaa ggcccgggtc cattatttca
     6181 ggcaatgaat cgaaagtaat aatgatttcc tccttacttt tcaggttatc cttatccgac
     6241 aggctgaaac gttcaaccag atcattgttt cgaagcaggt ttgtaatatc cccttcttca
     6301 cctgttgctt ccgttaacgg ataaaggttt gtgcacaaat aaaaatcgtt gttgcccgac
     6361 tggcatacct tttcctgtgc ttttaccggc caggcataca aactgatatc gttcacgcaa
     6421 tgggtttcaa gcgcttcatt tttcatggta atgcgaaatt ccttttccct ggtctttgtc
     6481 cgcggtaaag cgtccaggtc ggaatactcc cgtgaaggga aaaaagcatg tgaaaagcct
     6541 tcggcgtcag catgatggaa attatcccct ggatttatat aaaatgtagg gcacgatccg
     6601 aaacaagctt tggggacagt aaggcaaaac acaccaagag ccacatccag ccctgccagg
     6661 atgcttaacc ccgcgatccg tcctgattca tcgtatgcca gctttttgtt ggtttcgaaa
     6721 atggctacgc tgtcgatggg aatatccatc ggcccttcgg atacttttat ccgtcttttg
     6781 tcataagagg ttccgttccc ggaaatgaac cgctgtgttg aatcgacatt ccatgtatcc
     6841 cgcaatataa ccaccccgcc gtttttaagg tgggctttta aaaacggccg ggtctgaaga
     6901 ttcttttcct catgaatcag tctgttgatg tcgttgtaat tcgaccggaa atagtagggt
     6961 ggtgtggcgc aggaaaaggt ggcagccagg atcaccgata accaaaatgg ccgtttcagg
     7021 gtgttcagga atttccggtt catagctttg ctattaatgc atatgagtga tgatcttccg
     7081 gcatttcttt tcttaggatc ccattgatga agtgaatgcc catgaggaat catcctgatt
     7141 atcatcaggt ttcagtcggc aggctgctct aaacaggccc gggccgttat gaattaaaaa
     7201 acgaggtgac tagcaatccg ataaaatgtt ccgccggcaa tcccggcatt aacggaccgg
     7261 atccattcaa gccggcaggt atccggctat ccgcttcatg taaagttagt taaaatcagg
     7321 gatagctgca aataaatatc catttgcgac acgttggcaa acaatggaat atttcttcaa
     7381 aacagccatc acagcgtccc ctgcaacctg cctcggttta ttaagtaata agttcccgcc
     7441 gccacaaggt ttaccggcca gtttcgccga ctggcctgaa aacctgcctg catatttgct
     7501 gccggttccg gatccctgat tccgcaacca gtaattaaat cctttggtat tacctttttg
     7561 ctgttttctg tattaaatgc tggtagtctt acagtctttt tcatgtcagg gcccggcgcc
     7621 tccagggtga accttaaaaa gtacttaccc cggaagtacc gggttctggt ttgtaaagaa
     7681 cccttcagga tgtctcctga gaatgcctgg tttaagaacc cgtttcagcc cggtaagata
     7741 taaatctgta tattcgcatc ttacagttcc aggcttttca gccccgaatg gttcacttta
     7801 tttgccaata tgctcttccc cgaaataccg ttttaaaaga agggagaaga ttcgtgacac
     7861 ccgcagaact tatcagaaag tcatcgccat gaaaaaaaac cttgtcatct attattccaa
     7921 ttccggtagt aacaagtacc tggcaaacag gatcgccggg agtttagcct gtgatatcac
     7981 cgaaatcagg ccccgtttga atgcttttcc cctgttatta ctcttttcgt ccctgaaaag
     8041 aagttttgga attaaggcgc ttaaaccgaa ccccggtgaa tatgaacgga ttattttatg
     8101 cggacctgtc tggatgggga aactcatttc gcctttgcgc gatttccttt ttaagtacag
     8161 aaaaagcatc gacagcctgt attttgccag ttgctgtgcc agcagcgata tcgcaaaaga
     8221 cgaaaaatac ggtcacgggc tggtgttcaa tatggtcaga gagatcatgg gacaaaagtg
     8281 catccactgc gaagcctttc ctgtcggcct ggtagtgccg gaggacaaaa aggaggacgg
     8341 caacaccatc atgaaaacac ggttatcgga tgataatttc aacggggaaa tcctgttaag
     8401 gtttgaaaaa ttcatccaac tgcttgccta atagccattt cattgaaatg ttaaaccccc
     8461 gacggaacaa tcttacccgc cggggatgag gtttcagcgc ctgcatttaa accgatgcag
     8521 gcgtataggc atgaaaggcc gtcacatcaa atatcaggga ttccctgcta tcggcgcatc
     8581 acttttttca ctgctttgtt caccgataaa aaaagcacct gaaaccggat gctgaagcaa
     8641 ctttaattaa acattcaatt cctccgggag agcagaatat tccggatcat tcttgcatcg
     8701 gtccggattt tggtgttatc tttgactatc ttctgttata acccctaaaa aacgatagcc
     8761 gatgaaaaga acatggtttg tcaccgtatt gatcgccctg atgattgcgg tgatttctcc
     8821 cgttaaacta ctgggtcagg ataccaagcc cgataaagcc agggaaaaat caaaatccga
     8881 gaatgtcaac aagcctcaaa agaccgaaga caacgtcacc cccggaaata tccctgccgg
     8941 acaggaaggt aaggctgtga ccggtgaaaa ggaaaagggc taccaaaaaa ccggggatac
     9001 cggaaaaaaa gaaggctgga accaggagaa aaagaccggc gcagataaag ccggcatagc
     9061 caaagacaag aaaacccggg agaaggaaat gaaaaaagcc aggaagatga gagaagagaa
     9121 ggcccgcgct gccaagaaag aacgggaaaa gatgatgaag gagagagaaa aagaaagggc
     9181 aaaagcagca aaggaaaaga aagaagccag gaaacaacat tcgaagtaag gcatttccct
     9241 gaattactta acaatatccc ggatatttaa tccgatttgc caatccggac tgtagttccg
     9301 gaggcctgta tgtggcgccg gttttactgc ggataatcga acatgttttc cgggatggac
     9361 attcctgctc atctttacag gggtttccgg taaaatttgt gtttcatccc taacaggatg
     9421 ctgctatttt ttttcaattt gtcggttccc agttcgatca tccgggaaat tccctttggt
     9481 gacgaattat tgaagcctgc cgcattttta ggatttttta accttacaac aatcataaat
     9541 ttatctaaat ttgttaagag aatgaaataa aactttggat ccttcgtttt tctcaaggtt
     9601 aattattgcc attatttcaa aacgctttat tcaaaacaat ccacatattt ttcaaccaaa
     9661 tctatccaat tatgaaaaaa tcaatttcgt tgctggcttt gttgctattt ctgtcggcaa
     9721 gcctgtttgc ccaaaaaaca gagtatatca ggaaaaaaaa tgaagatctg tttttactga
     9781 aaaacaggct ggaaaaacct gataagaatg cagcaaaaga gaaacgtatt ccgcacttca
     9841 gggatatgat cacaaaaaaa caaaatcaca gccttaaatc aactgatgtt atcaaacaaa
     9901 ggctcgacag cattacagga ggctattggg atgactattc cagccagtgg ttaccggaat
     9961 ctaaagatga atttacttat gatgacaacg gaaataatac aaaattaatc gaatgtgaat
    10021 gggaacctga cgacaaccga tggatacctt atgataagca ggaatattcc tacgatgcag
    10081 aaggcaggat catcgagtat cttgattatg aatgggacga cgctaccagt caatgggaat
    10141 ctttctggaa aagttcgttc acctatgacg tcaacggcaa catgatccaa cttgttgatt
    10201 actattggag tgattacctt aataactgga catttgacac caaaaccgat atgacttacg
    10261 atgccgccgg gaacctggtg caggacattg aatacttctg ggatgaagac ataacccaat
    10321 ggattaattc aaccaaaggg gagtacactt acaataccgg caacaacatt atactggaaa
    10381 tcttctgtga ctgggatgaa aacagcagcg cctgggtaaa tttttgtaag acggaatact
    10441 cctatgatac cggcgacaag ttgattctga tattggacta tcaatggaat accaccgggt
    10501 ggattgcatc caataagatg gattacacct gggacctcaa cggccatgtg gttcaggaag
    10561 tcttttcttc atgggatgaa agttcctcct tatgggttta tgcctttaaa agtgaacata
    10621 tctacgactc agatggtaac ctgacgcagg aattatctta tctctgggag caatccgtca
    10681 gtcagtgggt cgctgaatca aaggatgaat atgaatataa taattcattt tccttctcca
    10741 acctgctgct gccattcttg ttcctgtatg acggagaaga tgttctggcc tatttcaatc
    10801 atatgcttac ccacatcaat aattatgttt gggaagacaa tcaatggata tttaactata
    10861 aaactgtttt ggactactcc ccgcaggaag tattatcaat aaacagtata agtgccggtt
    10921 ccaatcaggt atctcccaat cccgtaagcg attacctcac tgtaaccctg gataaaaact
    10981 acgacaaagc ccggtttgaa ttattcgaca tgcagggcaa aaaggtcctg tccgaaaacg
    11041 tgatgaatca tgagaaagta agccttcagg gtttcagcgc cggcatttat tattacgt
//
LOCUS       DAOPYV010000087        10959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_6879_length_10959_cov_13.844644, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000087 DAOPYV010000000
VERSION     DAOPYV010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10959
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6879_length_10959_cov_13.844644"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1697
                     /gene="atpD"
                     /locus_tag="PKI35_10230"
     CDS             192..1697
                     /gene="atpD"
                     /locus_tag="PKI35_10230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNX44803.1"
                     /translation="MAEKIVGKISQIIGPVIDVSFDENVPLPNIYEALEVTNDQGNVI
                     VLECQQDIGENAVRTVAMDSTDGLSRGMEVISTRRSISMPVGDAIKGRLFNVTGNAVD
                     GLDQVSHERSYSIHREPPKYENLTTSKEVLFTGIKVIDLIAPYSKGGKIGLFGGAGVG
                     KTVIIQELINNIAKHYNGFSVFAGVGERTREGNDLLRDMIEAGLVNYGDEFKKSMEAG
                     GWDLSKVDMKAMEQSLLTMVFGQMNEPPGARARVALSGLTVAEYFRDGEENSSGNDIL
                     FFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGIMQERITSTKRGSITSVQAI
                     YVPADDLTDPAPATTFAHLDATTVLSRKIFEKGIYPAVDPLDSTSRILAPDIVGEEHY
                     NTAQRIKQILQRYKELQDIIAILGMDELSDEDKQVVHRARRVSRFLSQPFHVATQFTG
                     IPGVFVSIEDTIKGFKMILDGEVDEYPESAFNLVGTIEEAIEKGKKMLAAQ"
     gene            1721..1957
                     /gene="atpC"
                     /locus_tag="PKI35_10235"
     CDS             1721..1957
                     /gene="atpC"
                     /locus_tag="PKI35_10235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNX44804.1"
                     /translation="MNIEIVTPDTTIFTGEITLAQLPGLDGSFEIMNNHAPLISVLKK
                     GRIKILNKQQQVQYFDVKGGVVEVLENKVLVLAE"
     gene            complement(2037..3059)
                     /gene="pheS"
                     /locus_tag="PKI35_10240"
     CDS             complement(2037..3059)
                     /gene="pheS"
                     /locus_tag="PKI35_10240"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNX44805.1"
                     /translation="MKAKIDRLLAEVQSFTAENAEMLEQFRIRFLGRKGFLQELFDEF
                     KTLPGDQKREVGQLLNVLKNAAQDKVNAFKEGPGTGPAAVSTVADLTRPVNFLESGSR
                     HPLSLVRNEIIRIFSRLGFSVNEGPEIEDDWHNFTALNFAPEHPAREMQDTFFIEKDP
                     DIVLRTHTSNAQIRVMEKTKPPIRAIFPGRVYRNEAISARAHCFFHQVEGLYIDKNVT
                     FADLKQTLHYFAKEMFDADTQIRLRPSYFPFTEPSAEMDISCNLCGGKGCGFCKYTGW
                     VEILGCGMVDPAVLENCGIDSKVYSGYAWGMGIERITNLKYQVKDLRMFSENDLRFLK
                     QFESAM"
     gene            complement(3210..3833)
                     /locus_tag="PKI35_10245"
     CDS             complement(3210..3833)
                     /locus_tag="PKI35_10245"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX44806.1"
                     /translation="MKSLIFSLFLTLLITMNLEAQNQTKVLIKTDYGDITVALYNDTP
                     LHRDNFIKLAKEGFYDNSIFHRVINRFMIQGGGAPAGKTEVDYRIPAEILSNHFHKYG
                     ALAAARMGDQVNPKKESSGSQFYIVQGQKYTEAQLQQFEARMGKKFTEEQLKAYTTVG
                     GTPHLDGAYTVFGEVVSGFEVIDKIAAVKTGAMDKPVQDVKMTMKVL"
     gene            complement(3856..4701)
                     /locus_tag="PKI35_10250"
     CDS             complement(3856..4701)
                     /locus_tag="PKI35_10250"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX44807.1"
                     /translation="MKIQIMKKSALFALVLMMAACNHKPKGDIVLISTEYGDIQVKLY
                     ENTPMHRDNFMKLAENGFYNNTLFHRVIPGFMIQGGDPASRHAKPGEFLGNGDTTYTI
                     PFEYIPAYFHKRGVLAAARESDDVNPERASSGSQFYIVQGRKFTDAGLDSADAKRERY
                     AKTGILINILKNRKDTLELSRFRNYIDNRDIENINKVIGKYSDQVNALYMQKPFARYT
                     PEQREAYKTIGGAPHLDGAYTVFGEVISGMDVVDKIAEIQRDKNDRPVKDIRMTMKII
                     SRQKR"
     gene            complement(4698..5288)
                     /locus_tag="PKI35_10255"
     CDS             complement(4698..5288)
                     /locus_tag="PKI35_10255"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNX44808.1"
                     /translation="MFLENTANVSQHRGWIEVICGSMFSGKTEELIRRINRARIAKQR
                     VEIFKPAIDKRYDDTNVVSHDASSIQSIPLETSSQLLMFIDNTDVVGIDEAQFFDPEL
                     PEICNRLAGMGIRVIVAGLDMDYLGKPFGPIPALMATAEYITKVHAICMRCGDLAHYS
                     HRIISSDKLVVLGEKDSYEPLCRKCFTEALNIKSRL"
     gene            5468..6352
                     /locus_tag="PKI35_10260"
     CDS             5468..6352
                     /locus_tag="PKI35_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44809.1"
                     /translation="MIQLIKEADDLIRKVVARYNGLYTQQGRASQIELDLMLDDIRQL
                     YEKFKLLGQLNMMPPAIRVDKPASAAPSPANPPAETWPEPKEERVMPVGEQQSETVAK
                     PLHQPPQSEEFNAEKTDRNTAPEKTPAPEHMPPPKENEPRREINHEQPAESRISPPPE
                     VKDSPKPSGTKTSRSPIQSEKPQHTLADTFHNEKKSLSETIVSPVADSSLGTRMQAQP
                     IADLKAAIGLNERFNFITDLFANDLLGFDEAVKQLNNAANREEALAFLASLGEKYHWD
                     DSMSALSRFTEFVHRRFM"
     gene            6360..7664
                     /locus_tag="PKI35_10265"
     CDS             6360..7664
                     /locus_tag="PKI35_10265"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNX44810.1"
                     /translation="MMRFLILIFLSLFLLTFNLIYGQDAGYARKVIDTLASPTMEGRG
                     YADRGDHKAAEYIAGQFRNTGLEPLYAGYFQEYSFPMNTFPGKVEVVVNNKKLVPARE
                     FLINASSPGIIGQFRVQSLAGKKIADPEKLKRFIRKNHHGKMVMIDKKGIEDKKVLFV
                     LDSLKNTNLRNAAGLIFVEDKKLTWSVMAGASLKPFPVVEIKQECLPRNLRKVKIVIE
                     NEFVNNYRTQNVLGFVKGSVQPDTFLVFTAHYDHLGRMGNEVYFPGANDNASGTAMLL
                     DLARHYSMPENKLYYSMAFFALSGEEAGILGSSYCAEHPPFPLNKIKFLVNLDMVGTG
                     SGGVTMVNATVFRDAYNRMVKINADNEYIRTVKERGESCNSDHCPFYKKGVPAVFIYS
                     MGDEFSEYHNPDDAAEKLPLSEYKDIFRLLSDFMDGFKPNDH"
     gene            7716..8390
                     /gene="rsmI"
                     /locus_tag="PKI35_10270"
     CDS             7716..8390
                     /gene="rsmI"
                     /locus_tag="PKI35_10270"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNX44811.1"
                     /translation="MSRLYLVPTPIGNLEDITLRALRILKEVDVILAEDTRTSGNLLR
                     HYEIDKKVMAHHQFNEHQTVETVVQRIAGGQSAALITDAGTPGISDPGFLLVRACLAA
                     GIEVECLPGATAFVPALVNSGFPCDRFYFEGFLPHKKGRQTKLKELAAYRQTFILYES
                     PFRLVKTLEQLGEFLGNERKACVSRELTKVYEENRRGSIADLVAWYKAHPPKGEIVIV
                     VEGCAE"
     gene            9121..9576
                     /locus_tag="PKI35_10275"
     CDS             9121..9576
                     /locus_tag="PKI35_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNX44812.1"
                     /translation="MPYTYEYPRPAVTVDAAVFRASESGYEVLLIRRGNEPFRGMWAL
                     PGGFVNMDETLEEAVARELKEETSLDGVEMEQLHTFSAVNRDPRHRTISTVFYGILNN
                     SSMEAEAGDDASETRWFHIGSLPPMAFDHNRVIGMAVKRIGKPVMPGEL"
     gene            complement(9581..10228)
                     /gene="elbB"
                     /locus_tag="PKI35_10280"
     CDS             complement(9581..10228)
                     /gene="elbB"
                     /locus_tag="PKI35_10280"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HNX44813.1"
                     /translation="MKKFAVILAGCGTTDGSEIHESVMTLYAIARHGASYHCFAPDIK
                     QHHVVNHFTGKEMAETRNVLVESARIARGNIRPLQELEIRSFDAVVFPGGFGVAKNLC
                     SYAFEGADCKVNPQVAEVIREAVTLRKPIGALCIAPIMLAKVIGDVEITLGQDSTASR
                     AAEKMGAHHKPTTHGQVITDSKHKLFTTPCYMLDATIADIANDADSLVKAMLEVM"
     gene            10314..>10959
                     /locus_tag="PKI35_10285"
     CDS             10314..>10959
                     /locus_tag="PKI35_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNX44814.1"
                     /translation="MCGRFSLTVNEAELNEFFETSGGDAPYVARYNCAPTQMLAVITN
                     EKPRQLSYFKWGLIPGWAKDPSTGHKMINARAETLSEKPSYKTALRTRRCLVPADAFY
                     EWRQNGDKIPYRIFLKNSRLFAFAGLWDRWRAPDGSEISSFTIITTGANEFMQAIHNR
                     MPVILNREDEKLWLGSNNAGELLELLKPYPGEMDAFPVSKLVNSPANDFPEVMVP"
BASE COUNT         2871 a         2643 c         2814 g         2631 t
ORIGIN      
        1 aaaaaaatca ccaacgggtt taacagtccc ggaaatttaa ggcagccgca tgcggatgtt
       61 cccgggatat ccgaaaataa aatcttgccg aaatgtagct gagcctaaac cagtaagtga
      121 atttttcgga agaagtttta tccgctgcaa ccaatatgtt tagtaaattt gcgccctcaa
      181 aacagaatgt tatggcagag aagatagttg gcaaaatttc ccagatcatc ggcccggtaa
      241 tcgacgtttc attcgacgaa aatgttcccc tccccaatat ttatgaagcc cttgaggtta
      301 ccaacgatca gggcaacgtc attgtgcttg agtgccagca ggatatcggc gaaaatgccg
      361 ttcgtactgt tgccatggac tcaaccgacg gattaagccg cggaatggag gtgatatcaa
      421 cccggcgttc catttccatg cctgtgggcg atgcaatcaa aggccggttg ttcaatgtta
      481 ccgggaatgc tgttgatggc ctggaccagg tatcgcacga acggtcatat agcatccacc
      541 gtgagccgcc aaaatacgag aatcttacta cttccaaaga agttttattc acaggtatta
      601 aggttattga cctgattgcg ccctattcaa aaggtgggaa aattgggttg tttggaggcg
      661 ccggcgttgg taaaaccgtc atcatccagg aactcatcaa caatattgcc aagcattata
      721 acggtttctc ggtatttgcc ggcgttggcg aacgcacccg tgaaggaaac gacctcttgc
      781 gcgatatgat cgaagcaggg ctggtaaatt atggcgatga gtttaaaaag agcatggaag
      841 ctggtggttg ggacctttct aaggttgata tgaaagctat ggagcaatca ttgcttacca
      901 tggttttcgg acagatgaat gaaccgcccg gagcacgtgc ccgtgtcgcc ctctcgggcc
      961 ttacggtggc cgaatatttc cgcgatgggg aggaaaacag ttcaggcaat gatatccttt
     1021 tctttgtgga taatatattc cgctttaccc aggccggttc cgaagtatct gcattgctgg
     1081 ggcgtatgcc ttcggctgtg ggctatcagc ctaccctggc aaccgaaatg ggtatcatgc
     1141 aggaacgcat cacctccacc aaacgtggtt ccatcacttc ggtacaggct atctacgtgc
     1201 ctgccgacga ccttaccgac cctgctcccg ctaccacctt cgcccacctg gatgcaacca
     1261 cggtgttaag ccgtaagatc ttcgaaaagg gtatttatcc tgccgttgac ccgctcgatt
     1321 caacctcgcg tatccttgca cccgatatcg tgggcgaaga gcactacaat accgcccaac
     1381 gcattaaaca gatactgcaa cgctataaag aattgcagga tatcatcgct atcctgggta
     1441 tggacgaact ttcggatgag gataagcagg tggtccaccg tgcccgccgt gtttcacgtt
     1501 tcctctcgca acctttccat gtagccaccc agtttacggg aatcccgggc gtatttgtca
     1561 gcatcgaaga taccatcaag ggattcaaga tgatcctcga tggtgaagtg gacgaatatc
     1621 ccgaatcggc ttttaacctg gtgggaacca ttgaggaagc catcgaaaaa ggcaaaaaga
     1681 tgcttgctgc ccaataaatg aatgcttaaa agtcacttca gtgaatattg aaatagtaac
     1741 ccccgatacc accattttta ctggtgagat taccctggcc cagctacccg gcctcgatgg
     1801 ttcgttcgaa atcatgaaca accatgcccc gctgatttca gtgctgaaaa agggacgcat
     1861 caaaatcctg aacaaacagc aacaggttca gtatttcgat gtgaaaggcg gagttgtaga
     1921 agtgcttgaa aataaagtgc tggtgctggc ggaataagaa aatcaggggg aaaaagaaaa
     1981 aggcaaaagc agggttttcc cgtttttgcc ttttgtttta taatctcttt cgtcgatcac
     2041 atagctgact caaactgttt caggaatcgg aggtcgtttt ccgagaacat gcgcaggtcc
     2101 ttcacctggt atttgaggtt tgtaatgcgt tcgatgccca tgccccaggc atagccgctg
     2161 tatactttac tgtcgatgcc gcagttttca agaactgccg ggtcaaccat accgcagcct
     2221 aaaatttcga cccatccggt gtatttgcag aagccgcatc cttttccgcc gcaaaggttg
     2281 catgaaatgt ccatctcggc cgaaggctcc gtgaatggga aataggatgg cctcaggcgg
     2341 atctgcgtat cggcgtcaaa catttctttg gcgaaataat gcagggtttg cttcaggtcg
     2401 gcaaacgtaa catttttatc gatgtacaac ccttccacct gatggaaaaa gcaatgggcg
     2461 cgggccgaga tggcttcgtt acggtaaacc cttcccggga aaatggcccg gatcggcggt
     2521 ttcgtttttt ccatgacgcg gatctgtgcg ttgctggtat gggtgcgcag cacgatatcg
     2581 ggatcttttt caatgaaaaa ggtatcctgc atttcacggg cagggtgctc cggagcaaag
     2641 tttaaagccg tgaaattgtg ccagtcgtct tctatttcgg ggccttcgtt gaccgaaaat
     2701 cccaggcgcg aaaaaatgcg gataatttca ttgcgcacca gcgaaagcgg atgacggctc
     2761 ccggattcca gaaaattcac cggacgggta agatcggcta ctgtggatac agcggccgga
     2821 cccgttcccg ggccttcctt aaaggcattc accttgtcct gcgccgcatt cttcaatacg
     2881 ttgagcaact ggccgacttc acgtttctga tcgccgggaa gcgtcttaaa ctcatcgaaa
     2941 agctcctgca gaaaaccttt ccgccccaga aaacggatac ggaactgttc cagcatttca
     3001 gcattttcgg cggtaaatga ttgtacttcg gcaagcaacc ggtcaatttt cgctttcata
     3061 acctgttaat tagaagaaat cctgccgggg tcattcctga caggattgca aaggtcggaa
     3121 aaaaaacaga aaatgaataa gcgggcgcca atctgttcaa acagaatcgg ctgcccgctt
     3181 gaatgggtta ccggggttga ccggatttat tacagtacct tcatcgtcat ttttacatcc
     3241 tgaaccggtt tatccatagc gccggttttt acggcagcaa tcttatcgat cacttcgaag
     3301 cccgaaacaa cttcgccaaa aacggtgtag gcgccgtcaa ggtgaggcgt tccgccaaca
     3361 gtagtgtaag ctttaagttg ttcttccgtg aattttttac ccatacgggc ttcaaactgc
     3421 tggagctggg cttcagtata tttctgtccc tgaacaatat aaaactgcga cccgctcgat
     3481 tccttcttcg ggtttacctg gtcgcccatg cgtgctgcag ccagtgcgcc gtatttgtgg
     3541 aagtggttgg acaggatttc ggccggaata cggtaatcaa cttcggtttt gccggcagga
     3601 gccccgccgc cctggatcat gaaacggttg atgacccggt ggaagatgga attgtcgtaa
     3661 aagccttctt tggctaattt gatgaaattg tcgcgatgca gcggtgtatc gttgtaaagg
     3721 gcaaccgtaa tgtcgccgta atcggtcttt atcagaactt tagtctgatt ctgggcttca
     3781 agattcatgg taattaaaag ggtgagaaaa agtgaaaaaa tcagggattt catgttttta
     3841 gatttttatg gttgattaac gtttttgccg gctgattatt ttcatcgtca tccggatatc
     3901 ttttacagga cggtcgtttt tatcgcgctg aatttccgcg atcttgtcaa caacatccat
     3961 gccactgatc acttcgccga aaacggtata tgcaccgtca agatgcggcg cccctccgat
     4021 ggtcttataa gcttcacgct gttcgggagt ataacgggca aaaggtttct gcatgtacag
     4081 agcattcacc tggtcggaat atttgccgat aactttattg atgttttcaa tatcccggtt
     4141 atcaatgtaa tttctgaacc tgctgagttc cagcgtgtct ttcctgtttt tcaggatgtt
     4201 gatcaggata cccgtttttg catagcgttc gcgtttggca tcggccgagt caaggccggc
     4261 atcggtaaat ttccttccct gcacgatgta aaactgtgat ccggaggagg ccctttcagg
     4321 atttacatca tcgctttcgc gggcggcagc cagaacgccg cgtttatgaa agtaggcagg
     4381 aatatactca aaagggatgg tataggttgt gtcgccgtta ccgagaaatt cgcccggttt
     4441 ggcatggcgc gaggccgggt cgccgccttg tatcatgaat cccggtatca cccggtgaaa
     4501 caaggtattg ttgtaaaaac cgttttcagc cagtttcatg aaattatcac ggtgcatagg
     4561 ggtgttttcg taaagtttta cctggatatc cccatactcc gtgctgatga gcacaatgtc
     4621 gccctttggt ttatggttgc atgctgccat catcaacacc agggcaaaca aggccgactt
     4681 tttcataatc tggattttca taatctggat tttatattca gggcttcggt gaaacatttg
     4741 cggcacaagg gttcatagct atccttttcg ccgaggacta ccagcttgtc gctgctgatg
     4801 atacggtgcg agtaatgggc caggtcgccg cagcgcatgc agatggcatg tactttggtg
     4861 atatattcgg ccgtagccat cagggcaggg atgggaccga aaggcttgcc aaggtagtcc
     4921 atgtcgaggc cggccacgat gacgcggatt cccatcccgg caaggcggtt acagatttcc
     4981 ggcaattcgg ggtcaaaaaa ctgggcctca tcaatgccaa ctacatccgt attgtcgata
     5041 aacatcagca gttgcgagga agtttccaac ggaatcgact gtatgcttga agcatcgtga
     5101 gatacaacat tcgtgtcgtc ataccgttta tcaatggcag gtttgaagat ttcaacccgc
     5161 tgctttgcga tccgggcacg gttgatgcgg cggatgagtt cttcggtctt gcccgagaac
     5221 atcgatccgc agatcacctc aatccatcca cggtgttgag aaacgttggc ggtattttcg
     5281 agaaacatga acggcgttgt ttatagtttg ctaattttaa gccttcatac ttagcgttac
     5341 aaacatacgg aattccgttt aaaacgggat aggtagccgt aacaggattt gttaacttta
     5401 tgaagcctat catgataaac tgaaatattt cggtagctga cagataaata tctcctagaa
     5461 catggaaatg attcaattaa ttaaggaagc cgacgacctg atcaggaaag tcgtggcacg
     5521 gtataacggg ctttatacac aacagggacg tgcttcacag attgagctgg accttatgct
     5581 cgacgatatc cggcaactgt atgaaaaatt caagctgctt ggccagctta acatgatgcc
     5641 tccggcaatc cgggttgata agcctgcttc ggctgctcct tcaccggcaa atcccccggc
     5701 ggagacttgg ccggaaccaa aggaagaaag ggtgatgccg gtcggggaac agcaatccga
     5761 aaccgtagca aaacctttac atcagcctcc gcagagtgaa gaattcaacg ctgaaaagac
     5821 agaccggaat actgcgcctg aaaaaactcc ggcgcctgaa cacatgccac ctccaaaaga
     5881 aaatgagccc cgcagggaga taaaccatga acagccggca gaatcccgga tttctccgcc
     5941 accggaagtc aaggactcac caaaaccctc cggaacgaaa acttccaggt ctccgataca
     6001 gtccgaaaaa ccgcagcata ccctggccga cacctttcac aacgagaaaa agtcgttgag
     6061 tgaaacgatt gtctcacctg tagccgattc aagcctgggc acacggatgc aggcacaacc
     6121 gatcgccgac ctaaaagccg ccatcggcct caacgaaagg tttaatttta tcaccgacct
     6181 gttcgccaat gacctgctgg gttttgatga ggctgttaaa caactgaaca atgctgcaaa
     6241 ccgggaggag gccctggctt tcctggcttc gttgggcgaa aaatatcatt gggacgactc
     6301 aatgtctgcc ttgtcaaggt ttacggagtt tgtccaccgc cgcttcatgt aagattcaga
     6361 tgatgagatt tttaatatta atcttccttt cgttattcct gctgaccttc aatctgatct
     6421 atggccagga tgccggttat gcccggaagg tcatcgatac gctggcatcg cccaccatgg
     6481 aaggccgggg gtatgcggac cggggcgacc ataaggcggc agaatatatc gccgggcagt
     6541 tcaggaatac tgggctggag cctttatacg ccggctattt ccaggaatat tcatttccca
     6601 tgaacacgtt tcccggcaag gtggaggtag ttgtcaacaa taaaaaactg gtccctgcgc
     6661 gtgagtttct tatcaatgct tcttcaccgg gaattatcgg gcagttccgg gtacagtcgc
     6721 tggccggaaa gaaaatcgct gatccggaaa aactgaaacg gtttatccgg aaaaatcatc
     6781 acggaaaaat ggtgatgatt gataaaaagg gcatcgaaga taaaaaagta cttttcgtgc
     6841 ttgattccct gaaaaacaca aacctgcgca atgctgccgg gttgatcttt gttgaagata
     6901 aaaagcttac ctggtcggtg atggcaggag cctcgttgaa acctttccct gtcgtggaga
     6961 ttaaacagga gtgtttgccc cgaaacctcc ggaaagttaa aatagtcatc gaaaacgagt
     7021 ttgttaacaa ttacaggacg caaaacgttc tgggatttgt caaaggaagc gtccaaccgg
     7081 atactttcct ggtttttacc gctcattacg accaccttgg acggatgggc aacgaggtat
     7141 actttcccgg cgccaacgat aatgccagcg gtacggccat gcttctcgac ctggcgcgcc
     7201 attattcgat gcctgaaaat aagctttact attccatggc tttctttgcg ctgagcggcg
     7261 aagaagccgg catcctcggt tccagttatt gcgcggagca tccgccattc ccgctgaata
     7321 aaattaagtt cctggtaaat ctggacatgg tcggaaccgg cagcggaggc gttacaatgg
     7381 taaatgcaac ggttttcagg gatgcttaca accggatggt gaagatcaat gccgacaacg
     7441 aatacatccg gaccgtgaaa gagcgcggcg aatcatgcaa cagcgaccat tgcccgtttt
     7501 ataaaaaagg ggtgcccgcc gtgtttattt attcgatggg ggatgaattc agcgaatatc
     7561 ataatcctga tgatgccgct gagaagttgc ccctgagtga atataaagat attttcaggc
     7621 tgttgtccga ttttatggat ggcttcaaac ccaatgatca ttaacttcag cctttccggc
     7681 ttgcagccgg caatttttaa accaatatca tccccatgtc ccgactgtac ttagtgccaa
     7741 cgccgatcgg aaaccttgaa gacatcaccc tcagggcgct caggatactg aaagaagttg
     7801 atgtgatcct tgccgaggat acgcgtacct cgggcaatct gctccgtcat tacgaaatcg
     7861 ataaaaaggt aatggcgcat catcagttca acgaacacca gaccgttgaa acggtcgttc
     7921 agcgtattgc cggcgggcaa tcggccgcgc tgatcaccga tgccggcacg cccggcattt
     7981 ccgacccggg tttcctgctg gtaagagctt gcctggcggc aggaattgaa gtggaatgtc
     8041 tgcccggagc caccgctttt gtccctgccc tggtaaattc cggtttcccg tgcgacaggt
     8101 tttattttga aggatttctt ccccataaga aaggcaggca gacaaaactg aaggaactgg
     8161 ccgcttaccg gcaaaccttc atcctttatg agtcgccgtt cagattggtg aaaaccctgg
     8221 aacagttggg cgaatttctc gggaatgaac ggaaagcctg cgtttcgcgc gagctcacca
     8281 aagtttatga agaaaaccgc aggggcagca ttgctgacct ggtcgcctgg tataaggccc
     8341 atccgccaaa gggggaaatc gtgatcgtgg tggaaggatg cgcggagtaa ggagcaggga
     8401 gaacggagta cggagaacgg agaacggagt acggagaacg gagcagggag aacggagcag
     8461 ggagtaggga gcgaggagca cggagaactt taggcactaa taacgcaaag cacaaaacac
     8521 agaacgcaaa acgcaaaaca cagaactcag aaatcagaat tcaaacggag caaggagaac
     8581 gaagaacgga gaatcttacc ttgcttaagc ctgtggggac cggcaggggg agaactcaaa
     8641 actcaaaact cagaactcag aactcaaaac gccaaaggcc aacggccaat agccagaagg
     8701 caaaggtcaa aagtttacct gcctgggtga ggttgtgggc aagacgacag ggcaaaggtc
     8761 aaaagtttac ctgcctgggg agggtgtggg taagacggca gggcaaagga gccaggagca
     8821 gggagcgagg agaagggcga acggagaacg gagaagggag aacggaaaac tttacctgcc
     8881 ggtgtatggc attgtgtgag aggcaggcag gggagaacgg agaactcaaa actcagaact
     8941 cagaacgcca aaggtcaata gccaatagct aacagccaac agctaaaaaa ggctggacta
     9001 cggaaatttt caaatttgca cattttcaaa tttttaaatt ccagaaccgg ctcctgagaa
     9061 cattaacgaa gaatagcgac taccaataac cataacaaaa gaatcagatc aatattagtt
     9121 atgccataca cctacgaata tccccgaccc gctgttaccg ttgatgctgc ggtattccga
     9181 gcttcggaaa gcggttatga agtactgttg atcaggcggg gcaacgagcc tttcaggggc
     9241 atgtgggcgt tgcccggcgg ttttgtcaat atggatgaaa ccctcgaaga agccgttgcc
     9301 agggaactga aagaggagac cagccttgac ggggttgaga tggagcagtt gcataccttc
     9361 agcgccgtca accgtgatcc gcgccacagg acgatcagta cagtatttta tgggattttg
     9421 aacaacagtt cgatggaagc cgaagccggc gacgatgctt cggaaacccg gtggtttcac
     9481 attggcagcc tgccgcccat ggctttcgac cacaaccggg tgatcgggat ggctgtaaaa
     9541 agaataggga aacccgttat gcccggagaa ttatgatgtc ttacatcact tccagcatcg
     9601 ctttcacaag gctgtcggcg tcgttggcga tgtcggcgat ggtggcgtcg agcatatagc
     9661 agggtgtggt aaacagcttg tgcttgctgt cagtgataac ctgtccatgc gtggttggct
     9721 tatgatgggc ccccattttc tcagccgccc tggaagccgt tgaatcctgg ccaagcgtga
     9781 tttctacatc gccaatcact ttggcaagca tgatcggggc gatacacagg gcgccgattg
     9841 gtttgcgcaa ggtcaccgct tcgcggatca cttcggcaac ctgcgggttt accttgcaat
     9901 cggcgccttc gaatgcataa gaacacaggt ttttcgccac cccgaaacct cccgggaaaa
     9961 ccacggcgtc aaacgaacgg atttccagtt cctgcaacgg ccggatattt ccgcgggcta
    10021 tccgcgcgct ttcaaccaga acattgcggg tctcggccat ctctttgccg gtgaaatggt
    10081 ttaccacgtg gtgttgtttg atatcggggg caaaacaatg gtatgatgcg ccatgcctgg
    10141 caatggcgta aagcgtcatc accgattcgt gaatttcgga accgtcggta gtaccgcaac
    10201 cggccagtat cacagcaaat tttttcatcc cgggaagcat tgtgattatt acacaaagat
    10261 aaggaacagg ggagcgaaat aaaacgtaaa tttattgggt aaatcagcct ttcatgtgcg
    10321 gacgtttttc ccttaccgtt aatgaagccg aactcaacga attctttgag acttcgggcg
    10381 gcgatgcccc ttatgtggcg aggtataact gcgctccgac gcagatgctg gcggttatca
    10441 ccaatgaaaa gccgcggcag ctaagctatt ttaaatgggg actgataccc ggctgggcca
    10501 aagacccttc gacaggtcat aaaatgatca acgccagggc ggaaaccctc agcgaaaaac
    10561 cttcctataa aacagcgctg cgtacccgcc gctgcctcgt tcccgccgat gccttttacg
    10621 aatggcggca gaacggagat aaaattccat accgcatttt cctgaaaaac agcaggttgt
    10681 ttgcctttgc cggcttgtgg gaccgctggc gcgcccccga cggcagcgag atatcatcct
    10741 ttaccatcat cacaaccggc gccaatgaat tcatgcaggc cattcataac cgcatgcccg
    10801 tgatcctgaa tcgtgaagat gaaaaattgt ggctgggcag caataatgcc ggtgaattgc
    10861 tggaactcct gaagccttat ccgggcgaaa tggatgcctt cccggtttcg aaactcgtca
    10921 actcgccggc caatgatttc ccggaagtga tggttccgg
//
LOCUS       DAOPYV010000088        10806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_7001_length_10806_cov_12.103804, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000088 DAOPYV010000000
VERSION     DAOPYV010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10806
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7001_length_10806_cov_12.103804"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(345..1397)
                     /gene="thiL"
                     /locus_tag="PKI35_10290"
     CDS             complement(345..1397)
                     /gene="thiL"
                     /locus_tag="PKI35_10290"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNX44815.1"
                     /translation="MISDPNPERTELSELGEFGLIDRLTENISLLNPSTLKGVGDDAA
                     VIDNQNKLTLVSTDLLIEGIHFDLTFTPLKHLGYKSAVVNFSDIYAMNGSPKQLFVGI
                     SVSNRFSAEALDELYSGIKLACKHYHVDLAGGDTTSSPQGLFLALTVTGVADKNKVVY
                     RNTAKPNDLICVSGDLGGAYMGLLLLEREKQVFLANPDMQPDLQGHDYIIGRQLKPEA
                     RKDIIESLDKAGILPTSMIDISDGLASEIMHICKGSKAGCRIYEEKIPIDAMTVMTAE
                     EFNISPVTAAMNGGEEYELLFTVRMEDYEKIREIEGITVIGHITGAEEGCNLVTGDNV
                     LVEIKAQGFDHMKEKE"
     gene            1627..4503
                     /locus_tag="PKI35_10295"
     CDS             1627..4503
                     /locus_tag="PKI35_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX44816.1"
                     /translation="MLRYRLPLILIAFLMINAVKAQDLRITGTLLNDDDHLPLVGATV
                     KLMPARDTTRFSYTTSDTQGKFEFSKLNRGSYILEVTYIGFDKLRKSVIASAAPKSEK
                     LVMKPTATTLKEVDVTSTTIRAEQKGDTVQFNANAFKVSQDATTEDLVRKMPGITIES
                     GTVKAHGEEVKKVLVDGKQFFGDDPSVALKNLPAEVVDKIQVFDKMSDQAAWTGFDDG
                     NAEKTLNIVTKQAKNTGQFGKFSAGYGNDDRYLAGVNMNIFNEKRRLTVLGLTNNINM
                     QNFSAEESSGSSGGGMMQGRGGPMGGFNMGPQNGITTVNSLGLNYDESWGKKLTVHGS
                     YFFNRNDNNLINKVNRQYFLSADSSQYYNENSLSDNTGNNHRMHGRIEYNVNDNNSFI
                     LSPRLTVQSSTSNSNVLALSGLDPELLNPALLLSSTDNQTNSQFNSYNFNNDLLFRHK
                     FSKKGRTFSINLGTGFSNRDGESVLNAMNQYYSSETGDSLQQQTKTLTKGITLSSNLV
                     YTEPVGENSQLQANYNISWSENSTDKKTYDYIFEQKEYSQYIDTLLSNVYSSDYITHR
                     PGLGYLFKTEKVNLNTGLTYQYALLKGDQQFPVDNNTDYDFGIFLPNLFLNYKITDRM
                     NLRVVYRGSTNAPSVKQLQAVVDNSNPLLLSAGNPSLKPEVRHFAMSRFSLQNKQKTS
                     NLFWMFFFQKTQDYIGNSTLLVQRDTVFFGQAVSRGAQLSMPVNLDGAWNTRTFLSLG
                     LPIRYIKCNLNFNSGLSYTQLPSLINGQENMSKTLGINEGAVLASNISQKLDFTLTYN
                     FNYNFVDNTLQSQSENNYIFQAASASVNWQFWKGFFIQNNVTYQAYNGISASYMMDYT
                     LWNAAFGTKFLKSNAAEIKISAFDILNQNKSVNRSVSDTYIEDSYTQVLQQYFMLTFT
                     YNLRNFNGKTPPSDRDRHGDFHRDGPPGPPPGGGFGPPGGGFGS"
     gene            4731..6179
                     /gene="sufB"
                     /locus_tag="PKI35_10300"
     CDS             4731..6179
                     /gene="sufB"
                     /locus_tag="PKI35_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNX44817.1"
                     /translation="MEKESNHIIDEVTRGEYKYGFYTDIETETAPKGLNEDTIRYISA
                     KKNEPEWLLEFRLKAYRHWITMKEPTWAHLSYPAIDFQDIIYFAAPKQKKSPQSLDEV
                     DPELLNTFNKLGISLEEQKRLSGVAVDAVMDSVSVKTTFSDTLAKLGIIFCSFSEAVQ
                     KHSDLVKQYLGSVVPFTDNFYASLNSAVFSDGSFCYIPKGVRCPIELSTYFRINAART
                     GQFERTLIVADEGAYVSYMEGCTAPQRDENQLHAAIVEIVALKDAEVKYSTVQNWYPG
                     DKDGKGGIYNFVTKRGICKGNHAKISWTQVETGSAITWKYPSVILMGDHSVGEFYSVA
                     VTNNHQQADTGTKMIHLGRGSKSTIISKGISAGQSNNSYRGLVRVVKRADNCRNFSQC
                     DSLLLGDKCGAHTFPYIRAENPTSIVEHEATTSKISDEQLFYCQQRGISAESAIGLIV
                     NGYAKEVLQRLPMEFAIEAQKLLSVSLEGSIG"
     gene            6222..6968
                     /gene="sufC"
                     /locus_tag="PKI35_10305"
     CDS             6222..6968
                     /gene="sufC"
                     /locus_tag="PKI35_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNX44818.1"
                     /translation="MLKIKNLSVAIKGKEILKGINLEVNPGEVHAIMGPNGTGKSTLA
                     YVIGGREGYEVTSGEITYKGMNLLELSPEERACEGIFLGFQYPVEIPGVSITSFLRTA
                     INEQRKYQGLDPVPPAEFLAMMEENKKLLDLQDKLANRSVNEGFSGGEKKKNEIFQMA
                     MLQPSLAILDETDSGLDIDALRVVATGVNNLRRPDNSAIVITHYQRLLDYIIPDVVHV
                     LYDGRIVKSGGKEMALELEEKGYEWIKNSL"
     gene            7005..8417
                     /gene="sufD"
                     /locus_tag="PKI35_10310"
     CDS             7005..8417
                     /gene="sufD"
                     /locus_tag="PKI35_10310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNX44819.1"
                     /translation="MDTSIQTYNLKERLIEQSLSRNIRFPGGDTPYVAGVRHKALEQF
                     REIGLPHPNLERWRGTDLSKAFETDYHLSFDPAGKGVDIEALFKCTVPHFETFLVAQL
                     NGWFVYNDGPLHTLPNGMVVGSLASAIHAHPGLIEKYYGNLTAQSDEGLTALNTAYAL
                     DGMIIYVPDNVSVEETLQMVNLIDSPENLMLHPRNLVILGKNAHLRLVQCDDSINHGT
                     TFINAVTEVFMDEDSSLDHYKLQNKDSHSTLINTMYFEQQQGSRLTSNAMSLNGGIIR
                     NSSCVKLDGENASADIYGLYLMDRNQHIDNRVFVDHAKPRCHSNELFKGVLDDHATGV
                     FNGHVLVRRDAQQTMAYQKNNNILLTDKATIDTRPFLEIYADDVKCSHGATVGQLDAN
                     AMFYIRSRGISEASARILLMYAFAAEIINKISIPELKQRIDDMVKKRLRGELSICDQC
                     VLQCSSQEKKADFKIDMSKI"
     gene            8451..9689
                     /locus_tag="PKI35_10315"
     CDS             8451..9689
                     /locus_tag="PKI35_10315"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNX44820.1"
                     /translation="MKTNTVVPVNIEKIRSDFPILGTEIYGKPLIYFDNAATTQKPRQ
                     VIDAVSEFYLFQNSNVHRGVHRLSTLATIAFEDTRKKIAEFLNARYQHEIIFTRGTTE
                     SINLVAFSFGEAFLKEGDEVIISSLEHHSNLVPWQQVCHRKKAVLRVIPVFENGELDL
                     EAYRTMLNPHTRMVAVNHISNSLGTINPVKEITRMAHEQGAAVLIDGAQAMSHGPVDV
                     QDIGCDFYAFSAHKMYGPMGIGGLYGREEWLSRMPPYQCGGEMIETVSFDETVFNGLP
                     YKFEAGTPNVADTAGFGAAIDYIRSVGWETIQEQESLLLQYGTEKLSQIEGVNIIGTA
                     PHKASIISFLLKNIHFYDAGTIIDHYGIAVRTGNHCTQPLMARLGILGTIRASFSFYN
                     TTEEIDRLVEAIEKVKEMFA"
     gene            9761..10198
                     /locus_tag="PKI35_10320"
     CDS             9761..10198
                     /locus_tag="PKI35_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNX44821.1"
                     /translation="MTIKETIGQIINEFGYFDDWMDKYNFLIEMGKSMPLIEEKYKVD
                     SNLITGCQSRVWLHANFEDGRIFFTADSDAVITKGIVYLLIRVFSGHSPDEILEAQTS
                     FLDSIGLKEHLSPTRSNGLLSMVKQIKLYALAFKTQQEQKEGN"
     gene            10310..10633
                     /locus_tag="PKI35_10325"
     CDS             10310..10633
                     /locus_tag="PKI35_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNX44822.1"
                     /translation="MYDLQQKAILTDQVILALKTIFDPEIPVNIYDLGLVYEVNINNE
                     GVAEITMTLTAPNCPVAEDLPVEVHDKVAAIPGLTGCNVTLTFDPPWDRAMISDAGLL
                     ELGFL"
BASE COUNT         2900 a         2647 c         2565 g         2694 t
ORIGIN      
        1 cgtcaggtgg caggcctgcc tgccttgcgc tgggctgtag gagggggcag ggtttgagat
       61 ctgcctgcca gcataatttt tcgtttttcg ttctcccttc tcctttcagc gttctgcatt
      121 ctgtgttttg tgttttgcgt tttccgttct ccctgctcct cgctccctgc tccctgctcc
      181 ttgccctctg ccttctgcct tctggctttt agccgtcggc ttttagcttt tagctaaagt
      241 tctccgttct gcgttctcct cgttccctgc tccctgctcc atgctattta accttcccct
      301 ttttctcccg gctcccggtt gtcactttat gagactaaaa acctttattc tttctctttc
      361 atatgatcaa acccctgtgc cttgatctcc accagtacgt tatccccggt aacgaggtta
      421 cagccttcct ccgcacccgt gatatgcccg ataaccgtga taccttcaat ttccctgatt
      481 ttttcataat cctccatcct gacagtgaac agcagctcgt attcttcgcc gccgttcatg
      541 gctgccgtaa caggcgaaat attgaactct tcggccgtca tcacagtcat ggcgtcgatg
      601 ggtatctttt cttcataaat gcggcatccg gcttttgaac ctttacatat atgcattatt
      661 tcggaggcca gcccgtcgct gatgtcaatc atggatgtag gaagaattcc ggctttatcc
      721 agactctcaa taatgtcttt tcgtgcctca ggtttgagtt gccttccgat gatgtaatcg
      781 tgcccctgga ggtcaggttg catatcgggg ttggccagga aaacctgttt ttcccgttcc
      841 agcaggagca gccccatgta tgccccgccc aggtcgcccg acacgcaaat caaatcattg
      901 ggctttgcag tgttgcggta aacaacttta tttttgtcag caacaccggt aacggtaaga
      961 gccaggaaca agccctgtgg gctcgaagtg gtatcgcctc cggccagatc gacatgataa
     1021 tgtttgcagg ccagttttat tccggagtat aattcgtcaa gggcttcggc cgaaaaacgg
     1081 ttcgaaaccg aaatcccgac aaataactgc ttcggtgaac cattcatggc atatatatct
     1141 gaaaagttga ccacagccga cttgtatccc aggtgtttga ggggagtgaa agtgaggtca
     1201 aaatgaatgc cttcaattag caggtcggtg cttacaagcg taagtttatt ctggttgtcg
     1261 attacggcgg catcatcgcc gacgcctttt aacgttgacg gattgaggag actgatattt
     1321 tcggtaagcc tgtcaatcag gccgaactcg ccgagttcac tcagttcggt tcgctcagga
     1381 ttgggatcag agatcataca tttcagggtt tccgcaaaaa tacatcagtc tggctaagca
     1441 accttgaaaa ttgttaaaag agatgcaaat gatgactaat tttgcgcccc cccaaaatgg
     1501 aaggacggaa agaaagcgct gaaaatgcag gacaggtttt aatgaaaaat tttacagtcc
     1561 gttaatcttt gggcgattcc ggccatctaa agtgtattgc tcttttttcg aattaaaagc
     1621 ctgcttatgc ttcgctaccg cctacccctt atcctgattg cttttctgat gatcaacgcc
     1681 gtcaaggccc aggatttacg aattacagga accctgttga atgatgacga ccacctgccc
     1741 ctggtcgggg ctaccgtgaa gctcatgccc gcgcgcgaca caacccggtt tagttacact
     1801 acttctgaca cacagggtaa atttgagttc tccaaactga accgaggctc ttatatcctt
     1861 gaagttactt acataggttt tgacaaactc aggaaaagtg tcatagcgtc tgctgccccc
     1921 aaaagtgaga agctggtcat gaaaccgacg gccacaacac tcaaagaagt tgatgttacc
     1981 agtacgacga tcagggcgga gcaaaaaggc gataccgttc agtttaatgc caacgctttt
     2041 aaggtctcgc aggatgccac caccgaagac ctggtcagga aaatgcccgg aatcaccatc
     2101 gaaagcggaa ctgtaaaggc gcatggcgaa gaagtgaaga aggttctcgt tgacggaaaa
     2161 cagtttttcg gcgatgaccc ttcggttgcg ctcaaaaatc ttccggcaga ggtagtggat
     2221 aaaatccagg tctttgataa aatgtcggat caggcagcct ggacgggttt cgatgacgga
     2281 aatgcagaaa agacgctcaa tatcgttacc aaacaggcaa aaaacacggg ccagttcggc
     2341 aagttttctg ccggttacgg caacgacgac cggtatctgg caggtgtcaa tatgaatatt
     2401 tttaatgaaa agcgccgcct tacggttctt ggattgacca acaacatcaa catgcagaat
     2461 ttctctgccg aagaatcctc aggctcatca ggcggaggca tgatgcaggg ccggggcggt
     2521 cctatgggcg ggttcaatat gggacctcaa aacggcatca ccaccgttaa ctcactcggg
     2581 ttaaattacg atgaaagctg gggaaagaaa ctgacggttc acgggagtta tttcttcaac
     2641 cgcaacgata acaacctcat caataaagta aaccggcagt atttcctttc tgccgattcg
     2701 agccagtatt acaatgaaaa cagcctttcg gacaataccg gcaataatca caggatgcat
     2761 ggcaggatcg agtacaatgt aaatgataat aactccttca tcctctctcc gcggctcact
     2821 gttcaaagca gcacatcaaa cagcaatgtg cttgctttaa gcggattgga tccggaattg
     2881 ctgaatcctg ccttgttgct gagctcgacc gataaccaga ctaacagcca gtttaacagt
     2941 tacaatttta ataacgacct gcttttccgg cacaaatttt ccaaaaaagg ccggaccttt
     3001 tccattaacc tgggaacggg attcagcaac cgtgacggtg aaagtgtatt aaacgccatg
     3061 aatcagtact attcatccga aaccggcgat tcattgcagc aacaaaccaa aacgcttacc
     3121 aaagggatta ccctgtcatc gaaccttgtg tacactgaac cggtaggtga aaacagccag
     3181 ttgcaggcca attacaatat ttcgtggtca gaaaacagca ccgataagaa aacctatgat
     3241 tatatttttg agcagaagga atactcgcaa tacattgata ctcttctttc gaacgtctac
     3301 agcagcgatt atatcacaca tcgcccgggg ctgggatacc tcttcaaaac agaaaaggta
     3361 aacctgaaca cgggattgac ctatcagtat gccctgctga aaggcgatca gcaatttccg
     3421 gtggataaca acacggatta cgatttcggg atttttcttc ctaacctgtt tctcaattac
     3481 aagatcaccg atcgtatgaa tcttcgtgta gtatatcgcg gaagtaccaa cgccccgtcg
     3541 gtaaaacagt tgcaggcggt cgtggataac tcaaacccgc tgttgttatc ggctggtaac
     3601 ccatcgctga agcctgaagt ccgtcatttt gctatgagcc gcttttcgct gcaaaacaaa
     3661 cagaaaactt ccaacctttt ctggatgttt ttctttcaga aaacacagga ttatatcggt
     3721 aattcaacct tgctggtgca gcgcgatacg gttttcttcg gccaggccgt ttcacggggc
     3781 gcacagctat cgatgcctgt aaaccttgat ggcgcctgga atacgcgaac atttctctcg
     3841 ctggggctcc cgatcaggta tatcaaatgc aatctcaatt tcaacagcgg ccttagttat
     3901 acgcagcttc ccagcctgat taacgggcag gaaaacatgt cgaaaacgct gggcatcaac
     3961 gaaggcgctg tgctggccag caatataagc caaaagctcg attttaccct gacgtataat
     4021 ttcaactaca attttgtcga taatacgttg cagtcgcaat cggaaaacaa ttacatcttc
     4081 caggcggcat cggcttcggt gaactggcag ttctggaaag ggttctttat ccagaacaac
     4141 gttacatacc aggcttataa cggtatttcg gccagttaca tgatggatta taccttgtgg
     4201 aatgctgctt tcggtacaaa gttcctgaag agcaatgctg ccgagatcaa aatcagcgcc
     4261 tttgatatac tgaaccagaa taagagcgta aaccgcagtg tttcggatac ttatatcgaa
     4321 gattcctata cccaggtact tcagcaatat tttatgctga ccttcaccta caacctccgg
     4381 aatttcaacg gaaaaacacc accttcggac cgcgaccggc atggagattt ccatcgcgat
     4441 ggccctccgg ggcctccccc cggcggtggt tttggccccc ccggcggtgg cttcggctcc
     4501 tgacgttaaa ttcaacgaat gaaaaacact gcttctgccc atcatgaaac cgggaaattc
     4561 tcccggtttt cttatttcag tattttttgt ccgaaacagg caacttcaga aaacaatatt
     4621 tatgataggt tgttaatttg atgcgttgca ggaaatttga tccgtgttta attgctaact
     4681 ttgctgccgc ttttatttaa actaaataag aatggattag cccgactgcc atggaaaaag
     4741 aatcgaatca cattatcgac gaggtaacta ggggcgaata taaatacggc ttttataccg
     4801 atatcgaaac agaaacggct cccaaagggc tgaatgaaga taccatccgc tatatatcgg
     4861 ccaagaagaa cgaacccgaa tggcttcttg aatttcgcct taaggcttat cgccactgga
     4921 ttaccatgaa ggagccgaca tgggcgcatc tttcttatcc ggccatcgac tttcaggata
     4981 ttatttattt tgcagccccg aaacagaaaa aatcgccgca aagtctcgat gaagtagatc
     5041 cggagttgct caatactttc aacaaactcg ggatatcact ggaagaacag aaaaggctga
     5101 gcggcgttgc cgttgacgcc gttatggaca gcgtctcagt aaaaacgacc ttcagcgata
     5161 ccctggcgaa gcttggcatt atattttgct cattcagcga agccgttcaa aagcattccg
     5221 accttgtaaa acagtacctg ggaagcgtag tgccctttac cgacaatttt tatgcttcgc
     5281 tcaattccgc ggttttcagt gacggatcat tctgttacat ccccaaggga gtccgctgcc
     5341 ccattgaact ttctacctat tttcgtatta atgccgccag gacaggtcag tttgaacgta
     5401 cgcttatagt cgccgacgaa ggcgcttatg tgagttacat ggaaggctgc acggccccgc
     5461 aacgcgacga aaaccaactt catgccgcaa tcgtcgaaat agtagccctc aaagatgccg
     5521 aagtcaaata cagcacggtt cagaactggt accccggcga taaagatggt aaaggcggaa
     5581 tctataattt tgtcaccaag cgcggcattt gcaagggcaa tcatgcaaaa atatcctgga
     5641 cgcaggtaga aaccggttca gccattacct ggaagtatcc cagcgtgatc ctgatgggcg
     5701 atcacagcgt tggtgagttt tactctgtcg ccgttaccaa caaccatcaa caggctgata
     5761 ccggtacaaa gatgatccac ctgggacgcg gcagcaagag caccatcatt agcaaaggca
     5821 tttcggcagg gcaaagcaat aacagttacc ggggacttgt acgcgtggta aaacgggccg
     5881 acaattgccg gaatttttcc cagtgcgatt ctctgctgct cggcgataaa tgcggcgcgc
     5941 atacttttcc ttatatccgt gccgaaaacc cgacttccat tgtagagcat gaagccacta
     6001 ccagcaaaat cagcgacgag cagctctttt actgccagca gcgcggaata agcgctgaaa
     6061 gcgccattgg gcttatcgtg aacggttatg ccaaggaagt tttgcagcgc ctgccgatgg
     6121 aatttgccat tgaagcgcag aaactgcttt ccgtgagcct cgaagggagc attggttagt
     6181 gtattgtcaa cgtattgaaa gtcattgaaa tccgacccta aatgttaaag attaaaaatc
     6241 tgagtgttgc catcaaaggc aaagagatcc tgaaaggaat aaacctcgag gtaaatccag
     6301 gggaagttca tgccatcatg ggaccaaacg gaacgggcaa gagcacgctc gcatacgtaa
     6361 ttggcggacg cgagggatat gaagtcactt cgggggaaat cacttacaaa ggcatgaatt
     6421 tgctggaact ttcgccggaa gaacgcgcct gtgaaggtat tttcctcggg tttcagtatc
     6481 ctgtggaaat tccgggcgtc agcatcacca gcttcctgcg gacagccatc aatgagcagc
     6541 gtaaatacca gggccttgac ccggttcctc cggctgaatt ccttgccatg atggaagaaa
     6601 ataaaaagtt actggacctt caggacaagc tggccaaccg ttcggtaaat gaaggctttt
     6661 cggggggaga aaaaaagaaa aatgaaatct tccagatggc tatgttgcaa ccaagccttg
     6721 ctatccttga cgaaaccgac tcaggcctcg atattgatgc cctgcgggtt gtggcaaccg
     6781 gcgtgaacaa cctgcgccgt cccgataatt cagccatcgt gatcacacac taccagcggc
     6841 ttcttgatta catcattccc gatgttgttc atgtgttgta cgacggccgt atcgtaaaat
     6901 cgggaggtaa ggaaatggcc ctcgagctgg aagaaaaagg ctatgagtgg attaagaatt
     6961 cattataagt acaactaaga gaaccggtta ccggaatcac tgaaatggat acaagcatac
     7021 aaacatataa cctgaaagaa cggctgatag agcaatcact cagccgcaat atccggtttc
     7081 cgggaggcga tacgccttat gtggcaggtg tccggcataa agcgctggaa caattcaggg
     7141 aaattggcct cccgcatccc aacctggagc gctggcgtgg taccgatctt tccaaagctt
     7201 ttgaaacgga ttaccattta tcgttcgatc cggccggaaa aggagtggat attgaagccc
     7261 ttttcaaatg cacggttcct cattttgaaa catttctggt tgcccagctt aacggctggt
     7321 ttgtgtataa cgacggcccg ctgcatacac tgcccaacgg gatggttgtc ggaagcttag
     7381 ccagcgctat ccatgcgcat cccggcttaa ttgaaaaata ctatggcaat cttactgcac
     7441 aatcggacga agggcttacc gcactgaata cggcttatgc cctcgatggc atgatcatct
     7501 atgtccccga caatgtctcc gtggaggaga ccctgcagat ggtgaacctg atcgattccc
     7561 ccgaaaacct catgctccat ccgcgcaacc tggttatact ggggaaaaat gcgcatctcc
     7621 ggctcgtcca gtgcgacgat tccatcaatc acggcactac cttcatcaat gcagtgactg
     7681 aagtgttcat ggatgaggat tcctcgctcg atcattacaa acttcagaac aaagacagcc
     7741 attccacgct catcaataca atgtattttg agcagcaaca gggaagccgg cttaccagca
     7801 atgcgatgag cctcaacggg ggcattatcc ggaacagctc gtgtgtaaaa cttgacggcg
     7861 aaaatgcatc cgctgatata tacggccttt acctcatgga ccgcaaccag catatcgata
     7921 accgggtatt tgtagaccat gcaaagcccc gctgccacag caacgaactg ttcaagggcg
     7981 tactcgacga tcatgcgacc ggtgttttta acggccatgt gctggtgagg cgcgatgcac
     8041 agcaaacgat ggcttatcag aaaaacaaca acatcctgct taccgataaa gccactatcg
     8101 atacccgccc attcctcgaa atttatgccg atgatgtaaa atgcagccat ggcgccaccg
     8161 taggacagct ggatgccaac gccatgtttt acatccgttc gcgcggcatc agcgaagcca
     8221 gcgcccggat cctgctgatg tatgcttttg cagccgagat catcaataag ataagcatcc
     8281 cggaattgaa acagcgtatc gacgatatgg tgaaaaaacg gcttcgcgga gaattgtcaa
     8341 tttgcgatca atgtgtgctg cagtgcagct cgcaggaaaa aaaggcagac tttaagattg
     8401 atatgtcgaa gatataattc cggcacttgc cgggaataac cctgttcatt ttgaaaacaa
     8461 ataccgtcgt gcctgtaaac atcgaaaaga tccggtctga tttcccgatt ctgggtaccg
     8521 aaatatatgg caaaccgctc atttactttg ataatgctgc aaccacgcaa aagccccgac
     8581 aggtaataga cgcggtttct gaattttacc tctttcaaaa ctcgaatgta caccgcggcg
     8641 tgcatcgcct cagtaccctg gcgacgattg ctttcgagga tacgcgaaaa aagatcgctg
     8701 agtttctgaa tgcgaggtat cagcatgaaa tcatttttac gcgggggacc accgaatcca
     8761 ttaatctggt tgctttctcg ttcggggaag catttctgaa ggaaggcgac gaagtcataa
     8821 tttcgagcct tgagcaccac tcaaacctgg ttccgtggca gcaggtgtgc catcgcaaaa
     8881 aagctgtcct gcgtgtgatc ccggtatttg aaaacggcga gcttgacctt gaggcttacc
     8941 gtacaatgct gaatccccat acccgcatgg ttgccgttaa ccacatttcg aattctctcg
     9001 ggaccatcaa ccctgtgaaa gagataaccc ggatggccca tgaacaggga gctgccgtgt
     9061 tgatcgacgg ggctcaggca atgtcgcacg ggcctgtgga tgtacaggat atcggctgtg
     9121 atttttatgc attttcggct cataagatgt acggcccgat gggcatcgga ggcctgtacg
     9181 gcagggaaga atggcttagc cggatgccgc cataccagtg cgggggtgaa atgattgaaa
     9241 ccgtgagttt cgacgaaacc gttttcaacg gtttgcctta caaattcgag gcaggaacgc
     9301 cgaatgttgc cgataccgca gggtttggcg cggccattga ttacatccgt tcggtggggt
     9361 gggagacaat tcaggagcag gaatcgttgc ttttacaata cgggacggag aaactctcgc
     9421 agattgaagg ggtgaacatc ataggaacag cgccgcataa ggcttccatt atatccttcc
     9481 tactaaaaaa catccacttt tatgatgcgg gcaccatcat cgatcattac gggatagcgg
     9541 tccgcaccgg gaaccattgc acccagcctc tcatggcgcg cctgggcatc ctgggaacca
     9601 taagggcttc gttctctttt tataatacca ccgaagaaat cgacaggctc gttgaagcca
     9661 tcgaaaaggt gaaggagatg tttgcctgag gaaggatcat gacgttttcg gtacggcatg
     9721 gcctctctta tcaaaaattg cgttgaaaaa caagtagaat atgaccatta aagaaactat
     9781 cggacagatt atcaacgagt ttgggtattt cgatgattgg atggacaagt acaatttcct
     9841 cattgagatg ggaaaaagca tgccgctgat cgaggaaaaa tataaggtag attcaaacct
     9901 gattacagga tgccagtcca gggtttggct tcatgccaat ttcgaagacg ggcggatttt
     9961 ttttactgcc gacagcgatg ccgtcattac caaagggatc gtgtacctgc tgatcagggt
    10021 gttttcgggt cattcaccgg atgagatact cgaagcccag accagctttc ttgattccat
    10081 tggcctgaaa gagcaccttt cgccgacgcg aagcaacggc ctgctctcga tggtcaaaca
    10141 gatcaaactc tatgccctgg cctttaaaac ccaacaggag cagaaagagg ggaattaaca
    10201 gacggagtat ggctggtagt ccctgcctga aaatcagcag gttgagaatt attccataaa
    10261 cgtttttttc aattggtggt cattaacctt tcaatctgat ctttgcccta tgtatgatct
    10321 gcagcaaaaa gccattctaa ccgaccaggt aatcctggcc ctcaagacga tctttgatcc
    10381 tgaaataccg gtgaatattt atgatctggg actggtttat gaagtcaaca tcaacaacga
    10441 gggtgtggct gaaatcacta tgacactgac tgccccgaac tgtcccgtgg ccgaggatct
    10501 gccggttgaa gtccatgata aagtagccgc tattccgggg ttgaccggct gtaatgtcac
    10561 cctcaccttc gatccgccct gggatcgggc tatgatcagc gatgcgggat tgctggagct
    10621 ggggttcctg taaaattctg aggggcatct ttccacgaat tggcacgaat tgatcacgaa
    10681 tggccacgaa aagattttaa agctaccgga gaaacgcttc gagataattc gtgtaacagg
    10741 cgaagcctgt ttcgtgtaat tcgtggttat agtatcacca cgaattggca cgaattgatc
    10801 acgaat
//
LOCUS       DAOPYV010000089        10733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_7080_length_10733_cov_13.121746, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000089 DAOPYV010000000
VERSION     DAOPYV010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10733
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7080_length_10733_cov_13.121746"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(442..1587)
                     /locus_tag="PKI35_10330"
     CDS             complement(442..1587)
                     /locus_tag="PKI35_10330"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNX44823.1"
                     /translation="MKENNDSTADLTRRIVKVIGGLLIIAGLIYMVWRFSFLIVYILI
                     AAAISFIGQPIVRFLDKLHIRKFKIPHGINSFIALLFLIACFSSLFAVFVPLILQQAE
                     TIAQIDIDKLTAQLEGPLANLQTQLTNLGVIPQGETLATFLTEKAKSLVDLASIGSLM
                     NGVLGIAGSLFVDIFSVVFIAFFFMKDENMFSEMVMAIPPEKHVKATEKVLKDSKMLL
                     HRYFIGVTIEILGGMTLITLGLLILGVPNALLLGFFGGLMNIIPYVGPILGTALGLVL
                     GLTNAMATGDFASLLPLILKIAGVFIVVHELDTNLFQPWVYASSVKAHPLEIFFAIII
                     GGSLGGIIGMLVAVPSYTVIRVIAHEFFSNFRMVKKLTEGMDAGEKG"
     gene            1768..3189
                     /locus_tag="PKI35_10335"
     CDS             1768..3189
                     /locus_tag="PKI35_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX44824.1"
                     /translation="METGEFREWAHRFADWMADYYDKIEEFPVKSQAKPGSIAAGLPL
                     SPPSGSESMERIFEDFRTIIMPGISHWQSPNFYAYFPANGSFASILGEMLTAALGSQC
                     MMWETSPAAAELEERVMDWLKQMTGLPASWSGVIQDTASTSTLAAILCARERYFDFRI
                     NEAGYSGHEGLRVYCSGETHSSVEKAVKISGIGRQHLVKVQTDDQYRMDVSALRKSII
                     RDLETGLKPCCVVATLGTTGCTSIDPLSEIAAVCREFNLWLHVDAAYGGTALLLPEYR
                     WMTDGIEQADSLVFNPHKWMFTNFDCSAYFVKDKESLVRTFEILPEYLRTGTYGLVND
                     YRDWGIQLGRRFRALKLWFVIRSFGVDGLKEKITRHLEMAKWFENKVISTPGFELMAP
                     RTMALVCFRYRPAGISEDETINELNEKLLQAINQSGRLYLSHTKLKGNYVLRMSIAQT
                     RTEQHHVETAWNLITETVKSLKS"
     gene            3204..5339
                     /locus_tag="PKI35_10340"
     CDS             3204..5339
                     /locus_tag="PKI35_10340"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNX44825.1"
                     /translation="MKITKLLFLIIAVFCLSEASHATITLPSTIGSNMVIQRESEFRL
                     WGWADPGQVLRIKATWYEDGLLQTTADNRGRWEITIPTGAAGGPYGIKIISGKEEVEL
                     LNILLGDVWICSGQSNMEYTINMFGGWKNGFEKDKKALVDYQYSNVRLFTVKKDTSNV
                     PKETCSGKWQVPDTVTVADFSATAWFYGLELNKRTGVPIGLVVSAWGGTAAEAWTPRA
                     VVGEDPSLVFYTASPNANASWPSKTGALYNAMINPLLSAKIAGAIWYQGEANVNDAPH
                     YRNLLMAMIGSWRKAWNSGDFPFYLVQLAPYTGYQDANSALLREAQFQTLSLKNTGMA
                     VTLDIAGDVGNIHPLNKQDVGKRLALWALANKYNKPQGDFSGPLFTKSETEQNTIRMF
                     FDHADSLYLKNTTTPEFYIAGDDRIFVPAKTRIDKNCVVAWNEEVKRPVAVRYAFRND
                     SKGNLYNRFSLPASSFRTDNWPYIADVAVLSVEKDPVTGKLKYALKTEAPKGIVHYST
                     TEIEPRCTSLEYTKPVEMPQSGILKTRVCTDGMPSDEVSVFHLTPSRALGAGVTYEKP
                     YSSKYTAGGAFGLVDGLTGSTNFSDGYWQGFQGDNLDMMLDMEKVQAVHTLTFGFLNS
                     PGSWIFMPRSVNISTSDDGKKFKAVDPVNIASQYDQAPKASRESVTVEVKRQTRFIKI
                     SVRNIGTCPAGHPGEGKPAWLFADEITVN"
     gene            5456..6352
                     /locus_tag="PKI35_10345"
     CDS             5456..6352
                     /locus_tag="PKI35_10345"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNX44826.1"
                     /translation="MSNLLTIIIVLVLLIFVTGIRIVRPTQRGLVERFGKYHHFAMPG
                     FNWVIPMGIDKMYKINITEKMINADPQEIITNDNLNARVDAQVYFRIKQDEESVKSSQ
                     YNVNSIEYQIVNLARTTLRNIIGTLTLKSANSERGRINKELHSTLFEETKNWGIDIVR
                     TELKEIDPPKDVQETMNKIVKAENEKIAAVDFATATETRADGEKRAEIRRAEGIKQAA
                     ILRAEGEAQAIALVNEAAEKYFVGNAQLLRRIQALETSLMNNAKIVVPTGSDLVNVLS
                     EMGGIIPLKGKAEGENPPVQKI"
     gene            complement(6570..8408)
                     /locus_tag="PKI35_10350"
     CDS             complement(6570..8408)
                     /locus_tag="PKI35_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44827.1"
                     /translation="MKLLPEQNRFTLTEKVVYLAASVFIVVLLIVRACKVLMVHDEIA
                     TFYYYVQSGRFNPFFTISDTNNHFLNSLLTWISFKVLGDSPLALRLPNLLFIPVYLFS
                     VLGIARFLKSVWMRIALFVSLVFTLHLVEFLALSRGYGMSYSLLFLCFLFAIRWLNSR
                     SDRDMVYCLISLFISSLANLAMINIYSLLLFFILLVKFREPERKTLRFLLTFTIAGIL
                     PFLFILAQVLYIKSESGLVAGNLNGFWATSMRTMISWLFEAEQPLSDLIPIVLLSLIA
                     LSITLICLKTRKITGHLETPYFLFGYLLTGSILSLIVLGAVFHVNYPDDRIATYLYVL
                     MILILVFGTDKLWELNGHKHSLLLALPLALLPVHFFIFLNTSYSGWYKYDVIPRRFYD
                     RVMQDCAPDKPAPTIGAHGLRIFCWSFLTYQNDGEANAIFFTTYPSYIADYQIVDLPA
                     VPLWNQYYDMLDYDPVSKRHLLKLKHSPKLSELAMLNVPAVRNCTKEFLLLSQGDARP
                     WRGKTLQADLNLAVNSGGKCLHSRIVVDVFNSENKSLRYEYLQLNWIRPGFPENGKRL
                     INSILIDQIPEEATGYKIYLWNIDKTNFGIEGHISVKERIVEKPVV"
     gene            complement(8426..8614)
                     /locus_tag="PKI35_10355"
     CDS             complement(8426..8614)
                     /locus_tag="PKI35_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44828.1"
                     /translation="MKITVFVLIFCAFMFQHAHAQKQPELIPYDDHGRWGSASYDNNP
                     ANFRLMPPTEIIRISISL"
     gene            8774..10000
                     /locus_tag="PKI35_10360"
     CDS             8774..10000
                     /locus_tag="PKI35_10360"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNX44829.1"
                     /translation="MKKSLLFFALLPVFTNFVRAQEEYGLTTKAMRIHDKVFTVDSHT
                     DTPLNLMVKGFNVSEDNSQAFRGSKVDFARMKKGGLDAAFFAVFISQGPRNPESNRKA
                     EMQALRIFDSVRAVVGRYPELAGLALTPADGYRLEKEGKRAVFIGVENGYPVGNDIRN
                     VKRFYDLGARYITLCHTKNNDICTSSTDTNNTERGLSAFGREVVAEMNRLGMMVDVSH
                     ISDKSFYDVLKVTRAPVIASHSCSRALCDNPRNLTDDMLKALARNGGVVQMCILSDYV
                     KPAPPNPARDSARNALSEKYHGFENLTDAEWEQARADWDKLRQTYPQPLASVADVCDH
                     IDHIVKVAGINHVGIGTDFDGGGGVTGCLDDSQMGNITLELVKRGYTTRQIRKIWGGN
                     LMRVMDEVAEKASKDI"
     gene            10023..10718
                     /locus_tag="PKI35_10365"
     CDS             10023..10718
                     /locus_tag="PKI35_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNX44830.1"
                     /translation="MIQVFYIRIQDIPSQAAEVEKLMTVLPLQAIRKLDNISNMPNKL
                     RSALGEILTRYAIQNVTGIGAKTLEVSYGTNGKPYLEKHPDVHFNISHSGDYIVCAVS
                     GVEVGIDVERCRAYNPGIAERFFSKPEYADLMRLEEDARRDYFFTLWTIKESYLKALG
                     RGLTKSLGSFTVTADDSGFRISGETSAADYSVFSDALPGDYKLAVSYKSALRPVQIRE
                     VTLHEISLLFTGL"
BASE COUNT         2924 a         2430 c         2764 g         2615 t
ORIGIN      
        1 agctgttttt ccaacggttc ttccgttccg ttaccgttct tctttgccat caatactttt
       61 tcttcttaaa atcccacaaa ttaaaagtaa aactcaaaag taataatttt gagcagatgt
      121 gtaagctaaa ttccttgcca aaattttatg atttcgagga gctcgtcaat tcccttcggt
      181 cgggtgaggc ggtatgaaat gcttttttat gggcttttta ttgcggggaa attcaaaggt
      241 attgctttat acaactgttg tttcggtcca ttttgtgctg tgcctttata aagcgttatg
      301 ggggtcgata aaaaacaaag tcctgtcgaa aaggatacgc agctatacta atccaaaaaa
      361 tactcaatta tttggagagc gtttttataa cgttttcagt attggcgtca aaaaggggaa
      421 aaatgcccgg tgtccatctt gttatccttt ctccccggca tccatacctt cggtcagttt
      481 tttcaccatt ctgaagttgc tgaaaaattc gtgggcaatc acgcggatga ccgtataact
      541 gggtacggca acaagcatcc cgatgatccc gcccagcgat ccgccgatga tgatggcaaa
      601 gaaaatttcg agggggtgcg cttttacgct gcttgcataa acccagggct ggaagaggtt
      661 ggtgtcgagt tcatggacta cgataaatac gccggcaatc ttcaggatca gcggcagcaa
      721 tgatgcgaaa tcgccggtcg ccatggcgtt cgtcaggccg aggacaaggc cgagagccgt
      781 ccctaatatg gggccgacgt aagggataat attcatcagt ccgccgaaaa atcccagcaa
      841 cagggcattg ggcacaccca ggatcagcag tccgagtgtg atgagcgtca tgcctcccag
      901 aatttcgatg gtaacgccga taaaatagcg gtgcagtaac attttcgagt ctttaagaac
      961 tttttcagtg gctttcacat gcttttcggg ggggatggcc attaccattt cgctgaacat
     1021 attttcatct ttcataaaga agaaggcgat gaaaaccacg gaaaagatgt cgacgaacag
     1081 ggatccggcg atgccgagca ccccgttcat cagggatccg atgcttgcaa gatcgaccag
     1141 cgatttggcc ttttcggtga ggaatgttgc aagggtttcg ccctggggga taactcccag
     1201 gttggtgagt tgggtctgca ggttagccag cgggccttcc agttgtgcgg tcagcttatc
     1261 aatatcaatc tgtgcgatcg tttcagcctg ctgaaggatc aacggaacaa ataccgcgaa
     1321 cagagaggag aagcaggcga tgagaaacag caaggcaata aaggagttga tgccgtgcgg
     1381 aattttgaat tttcggatgt gcagcttatc gagaaagcgg acgataggct ggccgatgaa
     1441 cgagatggcg gcggctatca ggatatatac gatgaggaag gaaaaacgcc aaaccatata
     1501 aatgagcccg gcaatgatca gcagacctcc gatgaccttc acgatacgcc gggtcaggtc
     1561 agcagttgaa tcattgtttt ctttcatatt gccctggata aatgctgtgt tgttgtttac
     1621 agggattgcc ctgaaaagtt tgtaaacaaa aatacggtaa atgtgacaga ttttacgtgt
     1681 gttcccgata tcgttgacaa agggcttaaa aatctgttca acttaccagg actttcttaa
     1741 ttttatgctt tgaataatta ttgatgtatg gaaacaggtg aattcaggga atgggcccac
     1801 cgctttgccg actggatggc cgattattat gataaaattg aagagttccc ggtgaaatca
     1861 caggcaaaac cgggcagcat agcagcgggt ttgccattgt cgcccccatc gggaagtgaa
     1921 agcatggaaa ggatttttga agacttccgc accatcatta tgccgggaat ttcccattgg
     1981 caaagtccta atttttatgc ctatttcccg gcaaacggta gttttgcttc catactgggt
     2041 gagatgctga cggctgcgct gggatcgcaa tgcatgatgt gggaaacctc gcctgccgct
     2101 gccgaactcg aagaaagggt gatggactgg ctgaagcaga tgaccggctt acccgcctcc
     2161 tggagcggag tgatacagga taccgcgagt acgtcgacgc ttgctgccat cttatgcgcc
     2221 cgcgaacgct atttcgattt ccggatcaac gaagccggtt actccggcca cgaagggttg
     2281 cgggtctatt gttcaggcga aacacattca tccgttgaaa aggctgttaa gatttccggt
     2341 atcggcagac agcatctggt aaaagtacag acagatgatc agtaccggat ggatgtttcg
     2401 gcgttgcgca aatccatcat ccgcgacctt gaaacggggc tcaaaccctg ttgcgtggta
     2461 gcgacgcttg gcactaccgg ctgcacatcc attgatcctc tcagcgaaat agccgccgtt
     2521 tgcagggaat ttaacctctg gcttcatgta gatgcggcct atggcggaac agccctgctg
     2581 ctgcccgaat accgctggat gaccgatggc attgagcagg ctgacagcct ggtgtttaat
     2641 ccgcacaaat ggatgtttac caatttcgat tgttcggcat acttcgtcaa ggataaagaa
     2701 agcctcgtcc gtacctttga aatattgccc gaatacctga gaaccggcac ctacgggttg
     2761 gtcaacgatt accgcgactg gggaatccag ttggggcggc gtttcagggc gctgaaactc
     2821 tggtttgtga tacgttcttt cggagttgac ggtctaaaag agaagattac ccgccacctg
     2881 gaaatggcga aatggtttga aaacaaggtc atatcgacgc cgggtttcga gctgatggcg
     2941 ccccgcacca tggcgctggt gtgcttcagg tatcgcccgg caggcatttc ggaggacgaa
     3001 accatcaatg aactgaatga aaaacttctg caggccatta atcaaagcgg aaggttgtac
     3061 ctttcacaca ccaaactgaa aggcaattac gttttgcgga tgtccatcgc acaaaccagg
     3121 actgaacaac accatgtgga aacagcctgg aacctgatta ctgaaaccgt aaaatctctg
     3181 aaatcataaa cttggaagca tctatgaaaa ttacaaaatt acttttcctg ataatagctg
     3241 ttttttgctt atcggaagcg tcgcatgcca caataaccct tccctccacc attggcagta
     3301 acatggtgat acaacgcgaa tcggaattca ggctctgggg ctgggccgat cccggacagg
     3361 tgctacggat aaaagctacc tggtacgagg atgggttgtt gcaaaccacg gcggataacc
     3421 gcggccgctg ggaaattacc atacccacgg gcgcagccgg cgggccttat gggataaaga
     3481 tcatcagcgg caaggaggaa gttgaattac tcaatattct gctgggagat gtctggatct
     3541 gcagcggcca gtcgaatatg gaatatacca tcaatatgtt cggaggatgg aaaaatggct
     3601 tcgaaaaaga caaaaaggcc ttggtggact accagtattc aaatgtccgg ttatttacag
     3661 ttaaaaaaga tacatcgaat gtcccgaaag agacctgctc gggaaaatgg caagtgcccg
     3721 atacggtgac ggttgccgat ttcagcgcaa ccgcctggtt ttacgggctg gagcttaaca
     3781 aaaggacagg cgttcccata ggcctggttg tctccgcctg gggcggtacg gccgccgaag
     3841 cctggactcc gcgtgccgtt gtcggagagg atccctcact ggtattttat acggcaagtc
     3901 caaatgcaaa tgcctcctgg ccttcgaaga ccggggctct ctacaatgcc atgatcaatc
     3961 cgctgctttc ggcgaaaatc gcaggcgcta tctggtacca gggcgaagcc aacgtaaatg
     4021 acgcgcctca ctaccggaac cttttgatgg cgatgatcgg aagctggaga aaagcatgga
     4081 attcaggcga ttttccgttc tatctggttc aactcgcccc ttacaccggg tatcaggacg
     4141 ctaactcagc cttgttgcgt gaagcccagt ttcagacgct ttcgctgaaa aataccggca
     4201 tggccgttac gctcgatatt gccggcgatg tcggtaatat tcacccttta aataagcagg
     4261 atgtagggaa aaggcttgca ttatgggccc tggcgaacaa atacaataaa ccgcagggcg
     4321 atttcagcgg cccgctgttt acaaaatcgg aaacagagca aaacacgatc cgcatgtttt
     4381 tcgaccatgc cgattccctt taccttaaga ataccactac accggaattt tacattgccg
     4441 gcgatgacag gatctttgta cctgcaaaaa ccaggatcga caagaattgc gttgtcgcct
     4501 ggaatgagga agtcaagagg ccggtggctg tcaggtatgc cttcaggaac gattcaaaag
     4561 gaaatttata caaccgtttt agtttgcctg cttcttcgtt ccggactgac aactggcctt
     4621 acattgccga tgttgctgtt ctgtcggttg aaaaagatcc ggttacgggg aaactgaagt
     4681 atgcattgaa gacggaagcc ccgaaaggaa ttgtgcatta ttccactacg gaaattgagc
     4741 cgcgttgtac ctccctggaa tataccaaac ctgttgaaat gccgcagtcg gggattctaa
     4801 aaaccagggt atgcaccgac ggcatgcctt ccgacgaagt cagcgtattc catctgacgc
     4861 cttcccgtgc cctgggcgcc ggggtaacct atgaaaagcc gtattcttca aaatatactg
     4921 ccgggggcgc attcggactg gttgacggac tgacgggatc gacaaatttt tcggacgggt
     4981 actggcaggg atttcaggga gacaacctgg atatgatgct ggacatggaa aaagttcagg
     5041 cagtgcatac cctgacattc ggttttctga attcacccgg ttcatggata ttcatgcccc
     5101 ggtcggtaaa catcagtact tccgacgatg gtaaaaagtt taaagcggtt gatccggtga
     5161 atatcgcttc gcaatacgat caggccccga aagcctcccg cgagagcgta actgtagagg
     5221 tgaagaggca aacccggttt ataaaaattt cggtccggaa tatcggaacc tgtcccgccg
     5281 gtcaccctgg agaaggaaaa cctgcctggt tgtttgctga tgagattacc gtaaattagg
     5341 ccgcgggaat tatttaatat caaccgtgtt aaacgtaatt aaactttcta tttttgtaaa
     5401 tagagaatgc gggaaataaa aacaatttgt taaaccatat taatacatct aagccatgtc
     5461 gaatttatta accatcatca tcgtacttgt cctcctgatc tttgttacag gaatccgaat
     5521 tgtaaggccg acccagaggg gattggtgga acgtttcgga aaatatcatc attttgccat
     5581 gcccggtttc aactgggtca ttccaatggg gattgacaag atgtacaaaa ttaatatcac
     5641 tgaaaagatg atcaatgccg acccgcagga aatcatcacc aacgacaatc taaatgcccg
     5701 ggttgatgcc caggtgtatt tcagaatcaa acaggatgaa gaaagcgtaa agagttccca
     5761 gtacaacgta aatagtattg aataccagat tgttaacctc gccaggacca ccttacgtaa
     5821 catcatcggt acgttaacgt tgaagtcggc caatagcgaa cgcggcagaa tcaacaagga
     5881 attgcactct acgttgtttg aagaaaccaa gaactggggt attgatattg ttcgtaccga
     5941 actcaaggaa attgatcctc ccaaggatgt tcaggaaacc atgaacaaga ttgtgaaggc
     6001 cgaaaacgaa aagattgccg ccgttgactt tgccactgcc accgaaacaa gggccgacgg
     6061 tgaaaagcgt gccgaaattc gccgcgccga agggattaaa caggctgcca ttttgcgtgc
     6121 cgaaggtgaa gcccaggcca ttgccctggt caacgaagcc gctgaaaagt acttcgtggg
     6181 gaatgcccaa ttgttaaggc gtatccaggc tcttgaaact tcattgatga ataatgccaa
     6241 aatcgtagtg ccgacaggtt ccgaccttgt gaatgtgctc agcgaaatgg gcgggatcat
     6301 ccccctgaaa ggtaaagccg aaggagaaaa tccgcctgtc cagaaaatat aacggctgtc
     6361 gcaatcatat agcaacagaa tccgggcggg agatgatgca ggaatactgc agaaggcctg
     6421 cccggatttt tttgttcatc atccggagag gatgatatca gccacaagca gaataaaact
     6481 ccgtttcaaa ggcagaattt tattcaggat cagttgtgtg ctttgaatcg gactgagggt
     6541 gaattcggga ctatcccgta tcaaaacttt tatacaaccg gcttttccac gatccgctct
     6601 ttaacggata tgtggccttc tatcccaaaa tttgttttat cgatattcca aaggtagatt
     6661 ttataccctg tcgcctcttc aggaatctgg tcgatcagga tgctgttgat gagtcttttg
     6721 ccgttttccg ggaaacccgg cctgatccag tttaactgca gatattcgta ccggagggat
     6781 ttattttcgc tgttaaaaac atccaccaca atccgcgaat ggaggcattt tccgccagaa
     6841 tttacagcca ggttcagatc ggcctgtaag gtctttcctc tccacggcct ggcatcgcct
     6901 tgcgatagca acaaaaactc cttggtgcaa ttccggacgg caggcacgtt caacattgcc
     6961 agctccgaaa gtttcgggct gtgtttcagc ttgagcagat gcctttttga tacgggatcg
     7021 taatcaagca tatcataata ttgattccat aacgggactg ccggtaagtc aacaatctga
     7081 taatcggcga tgtagctggg ataggtggtg aagaatatcg cgttggcttc gccatcgttc
     7141 tggtatgtta aaaatgacca gcaaaaaatg cggagtccgt gggccccgat ggtgggcgcc
     7201 ggcttgtccg gggcgcagtc ttgcatcacc ctgtcataga aacgtcgggg aatgacatca
     7261 tatttgtacc agcctgagta actcgtattc agaaaaatga aaaaatgaac cggcaacagg
     7321 gcgagcggta aagcaagtaa cagtgaatgc ttatgcccgt ttagttccca aagtttatca
     7381 gtgccaaaaa caaggataag gatcatcaat acatacaggt aggtggcaat gcggtcgtcg
     7441 ggataattta catgaaacac cgcgcccagc actatcaggg agaggatgct gccggtaagc
     7501 aggtacccga aaaggaaata gggcgtttcc agatgtccgg ttatttttct ggttttcagg
     7561 caaattaaag tgatgctaag agcgatgagc gacagcaaga ctatcggaat cagatcggaa
     7621 agaggctgtt ccgcttcgaa cagccaggaa atcatggttc gcatactggt ggcccagaag
     7681 ccgttcaggt ttccggctac caatcccgat tccgatttaa tataaaggac ctgtgccagg
     7741 ataaacagga aaggaagtat accggcaatg gtgaatgtca gcaaaaaacg cagtgttttt
     7801 ctttccggtt cccggaattt taccaaaagg ataaaaaaca acagcagcga gtagatattt
     7861 atcattgcca ggttagccag ggaggatatg aaaaggctta tcaggcaata aaccatatcc
     7921 ctgtccgacc gtgaattcag ccaccggata gcaaaaagga aacacaggaa caacaagctg
     7981 tacgacattc cgtagccccg ggacaaggcc aggaattcga cgagatgcag tgtaaaaacc
     8041 agggaaacaa agagggcgat gcgcatccag accgatttca ggaaccgggc aattccgagg
     8101 accgaaaaca ggtaaacagg aataaatagc aggttcggca agcggagcgc caggggcgag
     8161 tcgccgagta ccttaaacga aatccaggtc agtaatgaat tcaggaaatg gttattggta
     8221 tcggagatgg tgaaaaacgg attaaacctg ccgctttgga cataatagta aaaggtggca
     8281 atttcgtcat gtaccatcag gactttgcag gcccttacaa ttaacaggac gacgataaag
     8341 acggatgcgg caaggtatac gactttttct gtaagggtaa accggttttg ttctggtaat
     8401 aatttcatag gtatacgaat ggaaatcaga gcgaaattga tattctgatg atttctgtcg
     8461 ggggcatcag ccggaaattg gccggattat tatcatagct tgcagatccc catctgccat
     8521 gatcgtcgta aggaatgagt tccggctgtt tttgggcatg ggcatgctga aacatgaaag
     8581 cacagaaaat caggacaaat acagttattt tcatcaaatg ctataaaact tgatttcgca
     8641 aatatccaca atttgttcaa agcagaatac aaaacattca acaaattatc cggatggctg
     8701 tcgatggctt atttcgttga tgatgaaagc ctgttgtcat aacattgcta cttttgttac
     8761 agaaatgctt ctgatgaaaa aatcacttct cttttttgct ctgttaccgg tttttacaaa
     8821 ttttgtcagg gcccaggagg aatacgggct gacaaccaaa gccatgcgga tacacgataa
     8881 agttttcacg gttgattcgc acaccgatac tcctcttaac ctcatggtga aagggtttaa
     8941 tgtgtctgaa gataattcgc aggcgttccg gggatcgaaa gtggactttg ccaggatgaa
     9001 aaaaggcggc cttgatgcgg cattctttgc cgttttcatc agccagggcc cccgtaatcc
     9061 tgaaagtaat cggaaagcgg agatgcaggc gctcaggata tttgattccg ttcgtgcggt
     9121 tgttggccgt tatccggaac tggccgggct cgccctcacg ccggccgatg gttaccggtt
     9181 ggaaaaggaa gggaaaaggg ctgtctttat cggggtggaa aacggctacc ctgtcgggaa
     9241 cgatatccgc aacgtgaaac gtttttacga tctgggcgcc aggtatatca cactctgcca
     9301 taccaaaaac aatgacatct gcaccagttc caccgatacc aacaatacgg agcggggctt
     9361 gtcggctttt ggccgtgaag tggtggccga aatgaacagg ctgggcatga tggtggatgt
     9421 ttcccatatt tcggacaaat ctttttatga tgttctgaag gttaccaggg ccccggtcat
     9481 cgcatcccac agttgttcgc gggcgttgtg cgataacccg cgcaacctta ccgacgacat
     9541 gctgaaggcc ctggcacgta atggcggggt tgtccaaatg tgcatcctga gcgattatgt
     9601 gaaaccggcg ccgccgaatc cggcccggga ttctgcccgt aatgcgctga gtgaaaaata
     9661 ccacggtttc gaaaacctga ccgatgctga atgggaacag gcccgcgccg actgggataa
     9721 gctgagacag acgtacccgc agccgctggc ttcggtggcc gatgtatgtg accatatcga
     9781 ccatatagtg aaagttgccg gcatcaatca tgtgggtatt ggtaccgatt ttgacggtgg
     9841 cggcggcgtt accggttgcc tcgacgacag ccagatgggg aatatcaccc tcgaactggt
     9901 aaaacgcgga tacacgaccc ggcaaatcag aaagatctgg ggcgggaacc tcatgcgtgt
     9961 aatggatgaa gtggctgaaa aagcttcgaa agatatataa tcaccgaaag caatccccgg
    10021 aaatgataca ggtgttttat atcaggattc aggatattcc gtcacaagcg gctgaagttg
    10081 aaaagctgat gacggtgttg ccgcttcagg ctatccggaa actggataac atcagcaaca
    10141 tgcccaataa actaaggtcg gcccttggag aaatactgac gcggtatgcc attcagaacg
    10201 ttacaggcat aggtgcgaag acgcttgaag tgagttacgg gacaaacggg aagccttacc
    10261 tcgaaaagca tcctgacgtg cattttaata tttcacattc gggcgattat attgtttgcg
    10321 ctgtgtccgg cgtggaggtt gggattgatg tggagcgttg ccgggcatat aatccgggga
    10381 tcgcggaacg gtttttctcc aaaccggagt acgccgacct gatgcggctg gaagaggatg
    10441 cccgccggga ttattttttt actctctgga caatcaagga aagctacctg aaagccttgg
    10501 gccgcgggct taccaagagc ctgggctcgt tcacggttac cgctgatgat tccggattcc
    10561 ggatttcggg agaaacatcg gctgctgatt attcggtctt ttcggatgcc ttgccgggcg
    10621 attacaaact ggctgtcagc tataaaagcg cccttcgccc ggttcaaatc cgggaagtta
    10681 cgctgcatga aatatcgctc ttgtttaccg gactgtaatt tcgtaatttt att
//
LOCUS       DAOPYV010000090        10718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_7103_length_10718_cov_13.853137, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000090 DAOPYV010000000
VERSION     DAOPYV010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7103_length_10718_cov_13.853137"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PKI35_10370"
     CDS             complement(<1..774)
                     /locus_tag="PKI35_10370"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNX44831.1"
                     /translation="MKHKIIFFVFAALLVAFNGEAQDFWEVLSFPVGTNIHCIAVNNE
                     GDIFVGVGNDGAPGGVYRSLDNGHIWELVLNTGNFMVQSIAISQSGKIFVGRTGIYNF
                     MVSEDNGLSWNEIQLPSHNNVMKILCIDQDTVYVSLWEDGGGLVIRSPDCGQTWETVF
                     TSQNVSEYVSDIEITDEGIIYLSLRGYQSGQGGVYKSANNGVTWEFIGLFDCMVSSLA
                     VNSAGDLFAGSWGGSSLYNAGIYVIRNGHQDWDTLTGGQI"
     gene            1139..3268
                     /locus_tag="PKI35_10375"
     CDS             1139..3268
                     /locus_tag="PKI35_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNX44832.1"
                     /translation="MKVYKTNEIRNIALIGGAKSGKTTLAETMLFEGGLINRRGSVED
                     KNTVSDYREIELERQNSVSSTVLYAEYKGKKINIIDTPGFDDFIGEVVAALKVVDTAV
                     MVVNAQNGVEVGTEITWRHTAKYHTPVLFVVNQLEHEKANFEETLRQLKTQFGTHVTV
                     AQYPVGDGHAFHGIIDLLQMKMYQFRDGKVEVTDIPEAEKAKAEDLHNALVEKAAESD
                     ESLMEAFFENGSLTEEQIAKGIKAGIISRGFFPVFCASAKQNHGVARIMDFIVNSVPA
                     PDEMPGVNTLGGKLLKCNAADPASLFIFKTSIEEHLGEIAYFKVYAGEVAEAMDMING
                     NNSVKERIPQILITAGKKREKVEKATAGDIAATIKLKNSRTNETLNSPKNADDVIEPI
                     EFPSPKISFAVKVKNQGDEEKLGTRLNEMHKVDQTLEVEYSKELKQILIKGQGELHLN
                     IAKWHLEQLDKIPIDFIAPRIPYRETITKSANAMYRHKKQSGGAGQFGEVHMLIEPYT
                     EGMTFQQLYPVRGTDEQPLAWGGKLVFNNCIVGGAIDARFMPAILKGIMEKMEEGPLT
                     GSYARDIVINVYDGKMHPVDSNEMAFKLAGRNAFKEAFKNAGPKILEPIYDVEVIVPA
                     ERMGDIMTDLQGRRAIIMGMDSEGNYQKIKAKVPLAEMNRYSTALSSMTSGSGTYSLK
                     FAEYAQVPPDVQNALLKAYEEQEKDED"
     gene            complement(3339..4478)
                     /locus_tag="PKI35_10380"
     CDS             complement(3339..4478)
                     /locus_tag="PKI35_10380"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNX44833.1"
                     /translation="MENFTAYNPVKLHFGKGVIDTLAATVKPLGKKVLLVYGKGSVKR
                     SGLYHKISAQLETAGATIFEYGGIKSNPTVKDVDKAAAVGRDNEVDYIVAVGGGSVID
                     SAKIISITIPVSHSAWKFYEYRAKPVKAVPLVAVLTLAATGSEMNPFAVLQNEETGRK
                     DGYGSDLLFPLHSFLDPEVTYTVPRDYTAYGIADLIAHCLESYFGKGDCTLSDRFISA
                     IIADAMEWGPHLMNNLTGYDERAAIMYDATMALNLMTSYGKANGDWGVHDLGHILSLL
                     YDIPHGASLSILFPAWMKQLRDPAGSRISKLGNSLFGTPGVDDTIARFEAFFGTIGSP
                     VRLSQVGIGEDKFEEIIRVMVRNKAQGNHFKLTEEDYRGIFEWAK"
     gene            4634..5269
                     /locus_tag="PKI35_10385"
     CDS             4634..5269
                     /locus_tag="PKI35_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HNX44834.1"
                     /translation="MKKKIRKYYVVWRGRKPGIYDNWEECRKQVEGFPEARYAGFETP
                     EEARAALKGNPQDYFKRKPGGIPTRPMNSLYGVPEKESICVDAACSGNPGVLEYRGVY
                     TATGEEIFHRGPFPEGTTNIGEFLAIVTGLAWLKLHRLTIPLYSDSRNGLKWVKQKKI
                     NTTLERDAGTEKLFQTVDKALAWLHENGYDNKLLKWETEAWGEIPADFGRK"
     gene            complement(5334..6215)
                     /gene="atpG"
                     /locus_tag="PKI35_10390"
     CDS             complement(5334..6215)
                     /gene="atpG"
                     /locus_tag="PKI35_10390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNX44835.1"
                     /translation="MPSLKEVRIRIASVKSTQQITSAMKMVSASKLRRAQNAILKMRP
                     YASKLKEILQNLSASIGSSDQSPFTEQRSPEKVLLVVLSSNGGLCGAFNSNIIKSAVS
                     RIDQKWRSQDASGNLAIMTIGRKATEFFRKKGFNVVASHDGIYETLTWENVTIIAENL
                     MKDFSHHTYDRIEIIYNQFKNAAIQVIKVEQFLPVEPPEAEASSKLQSDYIFEPSKEE
                     IVRELIPKSLKIQFYKALLDSFASEHGARMTAMHQATDNATELLKQLNLIYNKARQAS
                     ITNEILEIVSGAEAQKG"
     gene            complement(6240..7823)
                     /gene="atpA"
                     /locus_tag="PKI35_10395"
     CDS             complement(6240..7823)
                     /gene="atpA"
                     /locus_tag="PKI35_10395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNX44836.1"
                     /translation="MAEIKPAEVTAILRQELAGFKTEAEVQETGSVLQVGDGIARVYG
                     LSNAQSGELVLFTKTGTKGIVLNLEEDNVGVVLLGESNDIKEGDTVQRTRLIASVKVG
                     EGLLGRVINTIGEPIDGKGEIKGDLYEMPLERKAPGVIFRQPVNEPLQTGLKAIDAMI
                     PIGRGQRELIIGDRQTGKSAIAIDTIINQRENYEKGKPVYCIYVAVGQKGSTVANIQK
                     TLEEHGAMPYTIIIMATASDPAAMQFYAPFAGAAIGEYFRDTGRPALVIYDDLSKQAV
                     AYREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKIIQSDTIAAQMNDLPASIKPLVK
                     GGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLETGLFNAGIRPAINVGISVSRV
                     GGHAQIKAMKKVAGTLKLAQAEYRELEAFAKFGSDLDAATKTILEKGSRNVEILKQLQ
                     YSPLPVEKQVAIIACGTRGLLQKVPLVSIRDFETEFLHHLEISHKDLMNQIRDGKLDD
                     EMLATIDQIAGEIARKYIK"
     gene            complement(7836..8393)
                     /gene="atpH"
                     /locus_tag="PKI35_10400"
     CDS             complement(7836..8393)
                     /gene="atpH"
                     /locus_tag="PKI35_10400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNX44837.1"
                     /translation="MSYTKITVRYAKALFDLALDNDLLERVEDDMALIDTVCHENKAL
                     VVMLHNPVISADKKQKVLSAIFSGNIHPLTMNFLSILSRKRREQYIDGIADAFVKIYR
                     DYKGITVARVTGAMPLDASEKQTILGILSKLTDKDVELIETIRTEILGGFIIQMDDYQ
                     IDQSVVTKIKNLKKDFEKNPYIKGF"
     gene            complement(8427..8921)
                     /gene="atpF"
                     /locus_tag="PKI35_10405"
     CDS             complement(8427..8921)
                     /gene="atpF"
                     /locus_tag="PKI35_10405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNX44838.1"
                     /translation="MELVKVDIGLLFWMTLSFGVVLYVLAKFAWKPIMKGIHEREESI
                     AKALNAAEDAKKAMLKLKAGNEELLLQAKEERDALMREARKLKDAIIEEARVKAGEEA
                     QRIIENARENIQYEKLAVINDLKNQVAAISIEIAEKILQKELSNREEQHQLTQKLLDE
                     VKIN"
     gene            complement(9009..9275)
                     /gene="atpE"
                     /locus_tag="PKI35_10410"
     CDS             complement(9009..9275)
                     /gene="atpE"
                     /locus_tag="PKI35_10410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNX44839.1"
                     /translation="MSLLAVLLQAVSNFAPFAQLGAGLGAGIAAIGAGMGIGQIGKSA
                     LESIARQPESTGDIRTNMIIAAALIEGVAFFAIVVCLLIVFLIK"
     gene            complement(9319..10482)
                     /gene="atpB"
                     /locus_tag="PKI35_10415"
     CDS             complement(9319..10482)
                     /gene="atpB"
                     /locus_tag="PKI35_10415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNX44840.1"
                     /translation="MSRLFKVLAALCITLCFSSVASASSTDSTSFGQTNTAAGQEARE
                     NQKKEFNAGEMIIEHITDAHEWHIATIGRTHISVPLPVILIDEGKVVVFMSSRLHHGT
                     AAYKGYKLETEGASKGKIVKVKEDGTMDENAKVPLDFSITKVVAQLFISLILLMVIFI
                     SIAGKYKKNPNRAPSGLQSLIEPLILFISDDLAIPAIGKKHYQKYLPYLLTIFFFIFF
                     SNLLGLVPFFPGGANLTGNLAVTMVLAVFTFLITNFSGNRMYWKDIVNTPGVPWWLKI
                     PLPLMPIVEIIGVLTKPFVLMVRLFANITAGHIIGLGFMSLIFIFGAMKPWLGYVVSP
                     VSVFFGIFMTFLELLVAFIQAYVFTLLSAIYFGMAIEEHHHEAKEEPVQEASR"
BASE COUNT         2786 a         2653 c         2582 g         2697 t
ORIGIN      
        1 aatctgcccg ccggtaagcg tatcccaatc ctgatggccg ttgcgaatca catatatgcc
       61 tgcattataa agcgatgagc ctccccatga accggcaaac aaatcgcctg cactgttgac
      121 tgctaatgac gaaaccatac aatcaaatag cccgatgaat tcccatgtaa cgccattatt
      181 tgcagattta taaacgccgc cttgaccgct ctgataaccc cgaaggctta aatatattat
      241 cccttcatca gttatctcaa tatcggaaac atattcgctt acattctggg aggtaaaaac
      301 agtttcccat gtctgtccgc aatcgggaga tcttatcact aaaccaccac catcttccca
      361 taaactaaca taaacagtat cctgatctat acatagtatt ttcattacat tgttatgaga
      421 tggaagttga atctcgttcc acgacaagcc attatcttct gaaaccataa aattgtaaat
      481 accggttctt cctacaaaaa tttttccaga ttgagaaatc gcaatagact gtaccataaa
      541 gtttccagta ttcaatacaa gttcccatat gtgtccgtta tcaagtgagc gatatacacc
      601 accaggcgct ccgtcattgc caacgcctac aaaaatatcg ccttcattat tgacagcaat
      661 acaatggata tttgtaccaa cagggaaaga caatacctcc cagaaatcct gcgcttcacc
      721 attaaaagca accaataaag cggcaaatac aaagaatata attttatgtt tcatcaatgg
      781 gatttgtttg cctgatatat aaatcgtacg aatataccat attatcacca ggtaaatacc
      841 atcgggtaag gagaaattat caaggggcgg ttgtgttttt attcgcagga cgacggcggc
      901 gctccggatc gggagtcgtc ccgcgaatgc cggtaagagg ataactgcgg atgcatccgt
      961 cacgcgaaac gatccggatg ctgacaaggc cgaaacagcc cggtttctat caggctgcca
     1021 ttcaatgcaa aatgcctcat gggtttttaa tatactattt gtaaatacca aactgtttca
     1081 taactttgca ccctttttac cggaataagt aattcattaa caataattca gcatacagat
     1141 gaaggtttac aaaacaaacg aaatcaggaa tattgcgctg attggcggcg ccaagtcggg
     1201 aaagaccacc ctggccgaaa caatgctgtt cgaaggcggg ttgatcaacc gtcgcggcag
     1261 tgttgaagat aaaaataccg tttccgatta ccgcgaaata gagctcgaaa ggcagaattc
     1321 tgtttcctcc accgttctct atgccgaata taaaggtaag aaaatcaata ttatcgacac
     1381 cccggggttt gatgatttca tcggcgaagt agttgccgcc ctcaaggtcg tggataccgc
     1441 ggtgatggtt gtaaatgccc agaacggcgt tgaagtaggt accgaaatta cctggcgcca
     1501 taccgcgaaa taccatactc cggtactgtt tgttgtaaac cagctcgaac acgaaaaagc
     1561 caattttgaa gaaacattgc gccagcttaa aacacagttc ggcacgcatg ttacggtagc
     1621 ccagtatccc gtcggcgacg ggcatgcctt ccacggcatc atcgacctgc tgcaaatgaa
     1681 aatgtaccag ttccgcgacg gtaaagttga agtgaccgat attccggaag ctgaaaaagc
     1741 caaagccgaa gaccttcaca atgccctcgt cgaaaaggca gccgaaagtg atgaatcact
     1801 catggaagcc ttcttcgaaa acggatcgct taccgaagaa caaatcgcaa aaggcattaa
     1861 agctgggatt atctcccgtg gctttttccc cgttttctgc gcttcggcaa aacaaaacca
     1921 cggtgtggcc cgcatcatgg atttcatagt aaattcagta ccggcgcccg atgaaatgcc
     1981 gggagtaaat acccttggcg gaaaactgct gaaatgcaac gctgccgatc cggcctcgct
     2041 cttcatattc aagacatcca ttgaagaaca cctgggcgaa atagcctatt tcaaagtgta
     2101 tgccggcgaa gtggctgaag ccatggacat gatcaacggc aacaactcgg tgaaggaacg
     2161 tatcccccag atactgatca cggccggcaa aaaacgcgaa aaggttgaga aagcaacggc
     2221 aggcgacatt gcagccacca tcaagctgaa gaactcacgt accaatgaaa cgctgaactc
     2281 gcccaaaaac gccgacgatg tgatcgagcc gatcgaattc ccgtcaccca agatcagctt
     2341 cgccgttaag gtaaagaacc agggcgatga agaaaaactg ggtacccgcc tcaacgaaat
     2401 gcataaagtt gaccagaccc tcgaagtgga atattcgaag gaactcaagc agatccttat
     2461 caaaggccag ggcgagcttc acctgaatat tgccaaatgg cacctcgaac aactcgataa
     2521 aattcccatt gactttatag ctcccaggat accttaccgc gaaaccatta ccaaatcggc
     2581 caacgccatg taccgtcata aaaaacaatc ggggggtgcc ggccagtttg gtgaagtcca
     2641 tatgctcatc gaaccttata ccgaaggaat gaccttccag cagttatatc ccgttcgcgg
     2701 aactgatgaa caacccctgg cctggggcgg taaactggtt ttcaacaact gtatcgtggg
     2761 tggcgccatc gacgcccgtt ttatgccggc cattctgaaa gggatcatgg aaaaaatgga
     2821 agaaggcccc ctcaccggtt cctatgcccg cgatatcgtc atcaatgtat acgacggtaa
     2881 aatgcacccg gtcgattcaa atgaaatggc ttttaaactt gccggccgta acgctttcaa
     2941 ggaagctttc aaaaatgcag gccccaagat ccttgaaccg atttacgacg ttgaggttat
     3001 cgttcccgcc gaacgcatgg gtgatatcat gaccgacctc cagggacgcc gcgccatcat
     3061 catgggtatg gacagcgaag gcaactatca gaaaataaag gctaaagttc cccttgccga
     3121 aatgaaccgc tattcaacgg cgctcagttc catgacctcg ggaagcggca cctattcgct
     3181 caaatttgcc gaatatgccc aggttccgcc cgatgttcag aatgccctgc tcaaagctta
     3241 cgaagaacag gaaaaagacg aagattaacc ctaaccttac ttccttatac aaaaagtccc
     3301 ggctcatcac cgggactttt tatattggaa tgagcgtatt acttcgccca ttcaaaaatc
     3361 ccgcggtaat cctcctccgt gagcttgaaa tgattgccct gggctttgtt tcgaaccatc
     3421 acccggatga tctcctcaaa tttatcttcc cctattccta cctgcgacaa ccgaaccgga
     3481 gagcctattg taccgaaaaa ggcttcgaac cgggcaatgg tatcgtcgac gccgggagtc
     3541 ccgaaaaggc tgttccccag tttcgaaatc cgggaacctg ccgggtcgcg cagttgtttc
     3601 atccaggccg ggaacagaat tgaaagcgat gcgccgtggg gaatatcgta aagaagcgat
     3661 aaaatatgcc cgaggtcatg cacgccccag tcgccgtttg cttttccgta cgaggtcatc
     3721 aggttaagcg ccatggttgc atcatacatg atggccgccc tttcgtcata accggtaagg
     3781 ttgttcatca ggtgcggccc ccattccatg gcatcggcga tgatggcgct gataaaccgg
     3841 tcgctcaggg tgcaatcgcc tttgccgaag taactctcca ggcaatgcgc tatcaggtct
     3901 gcaatgccgt aagcggtata atcgcgcgga acggtatagg tgacttcggg atcgagaaac
     3961 gaatgcagcg ggaacaataa gtcggaaccg tatccgtcct tacggccggt ttcttcgttt
     4021 tgcagcacgg caaaagggtt catttcgctg ccggtagccg ccagggtaag cactgcaacc
     4081 aaagggacgg ctttcacggg ctttgccctg tattcataga acttccaggc tgaatggctc
     4141 accgggatgg tgatggaaat gatcttcgcc gaatcaataa cgcttccgcc gccaacggct
     4201 acaatataat ccacttcgtt gtcgcgcccg acagcagcgg ctttgtcgac atcttttacc
     4261 gtcgggtttg atttgatgcc gccgtattcg aaaattgtcg cccctgctgt ttcgagctgg
     4321 gccgaaattt tatgatacaa tcccgaacgt ttcaccgatc ccttcccgta aaccagcaaa
     4381 acctttttac caaggggctt aaccgtggca gcaagggtgt cgataacacc tttgccgaaa
     4441 tgcagtttaa cagggttata agccgtaaaa ttctccataa tatttatctt tttattgatg
     4501 atgactttct ataaactgac cagaactgcc gtttgttcaa aattaggatt taatcatcaa
     4561 taccgtcatc cctttgctgc gatccggcct aaaaatttga ataatcgccg gatgtgacgt
     4621 attttcgcag gagatgaaga aaaagatcag gaaatattac gtagtatgga ggggccgcaa
     4681 gccgggcatc tacgataatt gggaagaatg ccgcaagcag gttgaaggat ttccggaagc
     4741 ccgctatgcg ggttttgaga cgcctgaaga agcccgcgcc gccctgaaag gaaatccgca
     4801 ggattacttt aaacggaaac caggcggaat accaacccgc ccgatgaaca gcctttacgg
     4861 cgttccggaa aaagagagta tttgcgtgga tgcggcatgc agcggcaacc cgggagtgct
     4921 cgaatatcgc ggcgtataca ccgccaccgg agaagagata ttccatcgcg gccctttccc
     4981 ggaaggcaca accaatatcg gcgaattcct ggctatcgtc acaggactgg catggctgaa
     5041 gctacaccgc ctcaccatcc cgctttacag cgattcgcgc aacggactga aatgggtaaa
     5101 acaaaagaag atcaatacca ccctggaacg ggatgccggt actgaaaagc tttttcaaac
     5161 cgtcgacaag gcgctggcct ggctgcatga aaacgggtac gataacaaac tccttaaatg
     5221 ggaaacggaa gcctggggtg aaatacccgc cgatttcggg cgcaaatgac agaaaaaaag
     5281 cggcatcaaa tcgtgccgct ttatttttgg tgcagaaatc aactttccgg ttatcagcct
     5341 ttctgggcct cggcgccgct gacaatttcc agaatttcat tggtaatgga agcctggcgg
     5401 gccttgttat aaatgaggtt gagctgtttg agcagttccg tggcattgtc ggtggcctgg
     5461 tgcatggcgg tcatacgggc gccgtgttcc gaggcaaaag aatcgagcag cgccttatag
     5521 aactgaattt tcagcgattt gggaatcagt tccctgacga tttcctcctt cgagggttca
     5581 aaaatgtaat cgctctgaag ctttgaagat gcttctgctt ccggaggttc aaccggcagg
     5641 aattgttcaa cttttatcac ctgaatagcg gcatttttga actggttgta aatgatttca
     5701 attctgtcgt aggtgtgatg cgagaagtct ttcatcaggt tttcggcgat aatggtaaca
     5761 ttttcccagg taagggtttc gtaaatcccg tcatgagaag ccacgacatt aaagcccttc
     5821 ttgcggaaga attcggtggc ttttcttccg atcgtcatga tcgccaggtt tcccgaagcg
     5881 tcttgcgacc gccatttctg atcgatgcgt gaaacggcgc tctttatgat attggaattg
     5941 aaagcgccgc ataatccgcc gttcgatgat aagaccacga gcagtacttt ttcaggcgaa
     6001 cgttgttccg tgaaagggct ttggtcggat gatccgatgc ttgccgaaag gttctgcagg
     6061 atttccttca gttttgatgc ataaggccgc attttgagga tagcgttctg ggcgcggcgc
     6121 aacttcgaag cagaaaccat tttcatagcg ctggtgatct gctgcgtcga ttttaccgag
     6181 gcaatcctta tgcgtacttc ttttaaacta ggcatgacat tccggggttc aaaccgtaat
     6241 tatttgatat atttccgggc gatttcgccg gctatctgat caatagtcgc cagcatttca
     6301 tcgtcgagtt taccgtctct tatctggttc atcaaatcct tgtgactgat ttcgagatga
     6361 tgcaggaatt cggtttcaaa atccctgatg cttacaagag gaaccttttg aagcagtccg
     6421 cgggttccgc aggcaataat ggcaacctgt ttttcgaccg ggagcggtga atactgcagt
     6481 tgcttgagga tttcaacatt ccttgatcct ttttcgagga tggtttttgt tgccgcatcg
     6541 aggtcggagc caaatttggc aaaagcttca agttcgcggt attcggcctg ggcaagttta
     6601 agggtcccgg ccaccttctt catggccttg atctgtgcat gtccgccgac cctggaaacc
     6661 gaaataccca cgttgatggc aggccgtata ccggcattga agagtcctgt ttcgaggaat
     6721 atctgcccgt cggtgatgga aataacattg gtagggatat aagcggaaac gtcgccggcc
     6781 tgggtctcaa tgatcggcag ggcggtaagc gagcctcctc ctttaaccag tggttttatc
     6841 gaagcaggga ggtcattcat ctgcgcggcg atggtatcgc tttgtatgat cttggcggca
     6901 cgttcgagca ggcgtgagtg caggtagaac acatcgccgg gatatgcttc acgtccgggc
     6961 ggacggcgga gcagcagtga gacttcgcgg taagcaacgg cctgtttcga aaggtcgtcg
     7021 taaattacca gcgccggacg accggtatcg cggaagtatt cgccgatagc agctccggca
     7081 aaaggtgcat agaactgcat agctgcggga tcggaagccg tggccattat aataatggta
     7141 taaggcattg ccccgtgctc ttcaagcgtt ttctggatgt tggcaacggt ggagcctttc
     7201 tgccccacgg ccacgtatat acagtaaacg ggtttaccct tttcgtaatt ttctcgctgg
     7261 ttgataatgg tatcgatggc aatggccgat tttccggtct ggcggtcgcc gatgatcagc
     7321 tcacgctggc cgcgtccgat agggatcatg gcatcgatag ccttgagccc ggtttgcagc
     7381 ggttcattca ccggctgacg gaatataacg ccgggggctt tgcgctcaag cggcatttca
     7441 tacaggtcgc ctttgatttc gcctttaccg tcgataggct cgccgatggt attgatgacg
     7501 cgccccagca atccttcgcc tacctttacc gatgcaatga ggcgcgtacg ctgaaccgtg
     7561 tcgccttcct tgatatcatt tgattcgccg aggagtacaa ccccaacatt gtcttcttcc
     7621 aggttaagca caatcccttt ggtgccggtc ttggtaaaca gtaccagttc gccgctctgg
     7681 gcattggaaa ggccataaac gcgggctata ccgtcgccta cctgcaatac cgatcccgtt
     7741 tcctgtactt cggcttcggt cttgaaaccg gccaattcct gtctcaggat cgctgttact
     7801 tcggcgggtt taatttctgc cataacaatt atcagttaaa atccttttat atacgggttc
     7861 ttttcgaagt ccttcttcag gttctttatt tttgtcacta cactttgatc tatttggtaa
     7921 tcatccattt gaatgatgaa accgccgagg atctccgtgc ggattgtttc aataagttca
     7981 acatccttat cggtaagttt cgacaggatt ccaagaatgg tttgcttttc cgatgcgtcc
     8041 aggggcatgg cgcctgtcac ccgggcaacc gtaattcctt tgtaatccct gtatattttc
     8101 acgaaagcat ccgcaatccc gtcaatgtac tgttcacggc gcttacgcga taatatgctc
     8161 aggaaattca tcgtaagcgg gtgaatgttt cccgaaaaaa tcgctgacag caccttttgc
     8221 tttttatcgg cgctgatcac cggattgtgc aacatcacaa ccagcgcttt attttcgtga
     8281 caaacggtat caatcagggc catatcgtcc tcaacacgct cgagtaaatc attatcgagg
     8341 gcaaggtcga acaaggcttt cgcgtaccgg accgttattt ttgtgtagct catttcgtat
     8401 cgtggatatt accgtcaggc tacggatcag tttattttaa cctcatcgag cagcttttgt
     8461 gtcaactgat gctgttcttc ccggttcgaa agttcttttt gcagaatttt ttcggctatc
     8521 tcaatggata tggcggccac ctggtttttc aggtcgttta tcacggcaag tttttcgtat
     8581 tgaatatttt cgcgggcatt ttcaatgatg cgctgcgctt cttcgccggc ttttacccgg
     8641 gcttcctcaa tgatggcgtc tttcagtttg cgggcttcgc gcatcaaggc gtcacgctct
     8701 tctttggcct gaagcagcaa ctcttcgttt ccggctttca gcttcaacat ggcttttttg
     8761 gcatcttcgg cggcattcag cgctttcgcg atcgactctt cccgttcatg aatacctttc
     8821 ataatcggtt tccaggcgaa ttttgccagt acatacagga caaccccaaa tgacaacgtc
     8881 atccagaaaa ggagcccaat atcaactttt accagttcca taatcaggtg tttttaactc
     8941 gtcacaagaa cggtcctttc cggcatcccg gccaatcgcc gggatgacac ggaacgaacg
     9001 taaccttatt atttaatgag gaacacgatg agcagacaaa caacgatggc gaagaatgca
     9061 acaccttcga tgagagctgc cgctatgatc atgttcgtac ggatatcgcc ggtgctttct
     9121 ggctgacggg cgattgattc gagcgcgctc ttaccaatct gaccgatgcc catacctgca
     9181 ccaatggcag cgataccagc acccaaccct gctcccagtt gtgcaaacgg ggcgaaattg
     9241 gatactgcct ggagtaatac ggctaacagt gacataatga ttgattttat ggtgattaat
     9301 taatgtttca ggttgatgtt agcgggaagc ttcctgaacg ggttcttcct tcgcttcatg
     9361 gtgatgttcc tcgatggcca tgccaaaata aatggccgat aacaaggtga agacatacgc
     9421 ctgaatgaag gcgaccagca gttcgaggaa ggtcatgaaa atgccgaaga agaccgaaac
     9481 aggtgaaact acatatccaa gccagggttt catggcgcca aagatgaaaa tgaggctcat
     9541 gaagccaagc ccgatgatgt ggcctgccgt gatgttggca aagagacgga ccatcaggac
     9601 gaaaggtttc gtcagtacgc cgatgatctc aacgatcggc atcaggggca acggtatctt
     9661 cagccaccag ggcactcccg gggtgtttac gatatctttc cagtacatcc tgttacccga
     9721 gaagttggta atgagaaaag taaagaccgc caaaaccatg gtgacagcca ggttccccgt
     9781 aaggttggcg cctcccggga aaaaagggac cagccccagc aggttgctga aaaagatgaa
     9841 gaaaaagatg gtgagcaggt aagggaggta tttttgataa tgctttttgc cgatggccgg
     9901 tatcgcgagg tcgtcgctga tgaacaggat cagcggttcg atgagtgact gcagaccgct
     9961 tggcgcgcgg ttcggattct ttttatactt gccggcgatg ctgatgaata tcaccatcag
    10021 caggatcagg ctgatgaaca actgtgccac cactttggtg attgaaaaat cgaggggaac
    10081 tttagcgttt tcatccatcg tgccgtcttc cttcactttg acgatcttcc ctttgctggc
    10141 cccttccgtt tccagcttat agcctttgta agcggcggtg ccgtgatgaa gcctgctcga
    10201 catgaagaca acaacctttc cctcatcaat aaggattacc ggcaggggaa cagaaatatg
    10261 ggtgcggcca atggtggcga tatgccattc atgggcatcg gtgatgtgct cgatgatcat
    10321 ctcgccggca ttgaattctt tcttctggtt ctcccgggct tcctgcccgg cagcagtgtt
    10381 cgtttgcccg aaagacgttg aatccgttga tgatgctgac gctacagaac tgaaacacag
    10441 tgtaatgcac aacgccgcca gtaccttgaa taatctactc atccgatcag aaattaccct
    10501 tttaaacggg agtgcaatat tacgaaattt tacggcaact ataccggtgg cataaaatgg
    10561 attttaatga ttctgaaacc ggtaaaattt gatgaaaccg gatgattccc aggccggcat
    10621 atctgtcatt gatgagggaa aaagggcttg ttccctggca gttgtctcca cgaatacaca
    10681 cgaatgaagc acgaatgaac acgaataaat gttgtgag
//
LOCUS       DAOPYV010000091        10573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_7271_length_10573_cov_9.860145, whole genome shotgun sequence.
ACCESSION   DAOPYV010000091 DAOPYV010000000
VERSION     DAOPYV010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10573
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7271_length_10573_cov_9.860145"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..781
                     /locus_tag="PKI35_10420"
     CDS             44..781
                     /locus_tag="PKI35_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HNX44841.1"
                     /translation="MERLRITAVKYYNTLPFIYGITRSGLLSGFDLSLDVPSECARKL
                     AAGEADLGLIPVGALPSIPDYRIISDFCIGAVGDVQSVILLTDSELSSLTKIYLDTDS
                     RTSVVLVKVLAERLWKINPAWESASNFTGALKPGEGIVAIGDKTFSLRPKFRCCYDLA
                     GEWIRMTGLPFVFAVWASRKPLNDSFLRSLNNALGWGVEHKNESVSIAADLIISKEEL
                     LSYLEQAIHYPLDDKKRQGMKLFLSML"
     gene            complement(834..2750)
                     /gene="yidC"
                     /locus_tag="PKI35_10425"
     CDS             complement(834..2750)
                     /gene="yidC"
                     /locus_tag="PKI35_10425"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNX44842.1"
                     /translation="MSKSNIIGLIVIFAMVIGYSIWMSPSQEDIQKARHKADSIALVQ
                     YKDSIAQAKLLAEKDSVEKAAVVVPNKPDTLENLQVKSNQYGTFASASSGVQKFYTLE
                     NEVLKLKLSARGGRIWSAELKNYKTYNKLPLILFEGDSNRFDLEFNTEDLKSINTGDL
                     YFVPSPVTATPGDSLTIAGKDSVQFAFRLNAASDSLGAGDHYIEYLYTLYKDEYMIGF
                     RIRMVGMENIIARNTRDIALSVSTDMLRQEAGFKTERDQSTIYFMDNTGDFDYLKETK
                     DSKENITSAVKWVSFKTQFFSAAMIASDAFNSADIATTSYQDVNGRLLKNMKTAFLVS
                     YDPGQNQDYKMQCYYGPNKFRTLKKYDLKLERQIPLGWSFRPIAWFNKYVVVLIFNWL
                     EQYGLNYGIIILIMTIMLKIVLFPIAYKTYMSSAKMRVLKPEVDEINARYPKQEDAMK
                     KQQATMALYKKAGVNPMSGCVPMLLQFPILVAMFRFFPASIELRQQSFLWAHDLSSYD
                     SIYNLPFSIPFYGDHVSLFTLLMTVSTIIYTKINNDMMGSTNQMPGMKAMMYLMPVMF
                     LGFFNNYSAGLSYYYLLANVFTFAQMFLIRRFVDEEKLHKRIQENKAKPVKKTSFQKR
                     LEEMAKKQGVNVKR"
     gene            complement(2877..4496)
                     /locus_tag="PKI35_10430"
     CDS             complement(2877..4496)
                     /locus_tag="PKI35_10430"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNX44843.1"
                     /translation="MSEPKYIFTTGGVASSLGKGIISSSLAKLLISRGFRVTIQKLDP
                     YINIDPGTLNPYEHGECYVTTDGAETDLDLGHYERFTNVPTSQANNVTTGRIYQAVIE
                     KERRGDYLGETVQVIPHITDEIKSRIQTLGLSGKYDFVITEIGGTVGDIESLPYIEAV
                     RQMKWEMGNRCVVIHLTLVPYLAASGELKTKPTQHSVKTLLESGVQPDIIVCRTEKHL
                     TQSLRNKIALFCNVNPSAVIESIDAETIYDVPLLMRDEKLDQVVLEKMQVPIRKEPDL
                     ARWEEFLFRLKHPVAEVQIGLIGKYVELKDAYKSISEAFVHAGAANQLKVKVKMIHSE
                     SINSGTVKHLLKDLDGILVAPGFGERGIEGKILAIRYARENKIPFLGICLGMQCAVVE
                     FGRNVLKMNDAHSTEMNPKTPYPVIDIMEEQKKISNKGGTMRLGAYPCTIREDSKSYE
                     IYGTADITERHRHRYEFNNKYLDEYNKAGMLAVGINRQNDLVEMIELKDHPWFIGVQF
                     HPEYRSTVANPHPLFVSFVKAAEQFKKTETK"
     gene            complement(4897..5280)
                     /locus_tag="PKI35_10435"
     CDS             complement(4897..5280)
                     /locus_tag="PKI35_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX44844.1"
                     /translation="MNVLIAEDNHLNQQLMTLFMKRFDWRYTIVSDGFGAVDACRTGV
                     FDVVLMDVDMPGMDGIEATTCIRHFDRSTPIVAVTAFTDEVTRAKCLEAGMDRFLSKP
                     LTKEQILSVVTELVPVNMHYAVKYA"
     gene            complement(5517..6383)
                     /locus_tag="PKI35_10440"
     CDS             complement(5517..6383)
                     /locus_tag="PKI35_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNX44845.1"
                     /translation="MRQLKISKSITNRETASLDKYLQDIGKEGLISAEEEVELARKIR
                     SGDERALDKLCRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKIKKETSRLEQKFERTPSADEIAA
                     ALEIPEYKIDAALKISTKYISMDAPLAEDEETKFIDVFVDEDAPYTDAGLMRESLAQE
                     IQRSLSTLSDKERDVINLYYGIGIPHGLTLEEIGARFDLTRERVRQIKEKAIRRLKHN
                     SRSKLLKAYLGQ"
     gene            complement(6532..7980)
                     /locus_tag="PKI35_10445"
     CDS             complement(6532..7980)
                     /locus_tag="PKI35_10445"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNX44846.1"
                     /translation="MKKYAGYLMIAILGGFISLGSYALIQKRSEKLQGVPQQPIPVTY
                     ASMSGDGSVLPDFTTAANMSIHAVVHIKTTYERKNSYYDYYFDLHDFFNNRQPGIQQP
                     LEGSGSGVIVSPDGYIVTNNHVVQDATTVTVVLNDKREFDAKILGTDPSTDIAVVKID
                     EKDLPYIVYGNSDDLKIGEWVLAVGNPFNLTSTVTAGIVSAKARNIDILGMPDGSAIE
                     SYIQTDAAVNRGNSGGALVNTRGELVGINAAIASGTGYYAGYSFAIPVNIAKKVVADL
                     IEFGAVQRAFLGVSIRDIDSKFAGENNIRQLRGVYISDVTDNSSASEAGIRKGDVITA
                     IDGINVNSTSELLEQVGQYRPGDKAKVSVLREGSEKDFQVTLKNKGGTTSVIAKEEQD
                     NVERLGASFGIVSPEMRKKLGISNGVQVTALQDGILKEAGIKVGFIITEVDNQPVKSA
                     KDIENAIENKKGGVLIEGIYPNRTRAYYGFGL"
     gene            8254..9039
                     /gene="dapF"
                     /locus_tag="PKI35_10450"
     CDS             8254..9039
                     /gene="dapF"
                     /locus_tag="PKI35_10450"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNX44847.1"
                     /translation="MELLHFDKYQGTGNDFILVDDRKQHRDFNGKTIVKLCDRRFGIG
                     SDGLLLLRERQGYDFEMLFFNPDGSRATFCGNGARCISAFAASLGVIQDEASFLAPDG
                     AHKASVLSRNGNDSLVSISMIDPVIYHHSEDYTYLNSGTYHVVKFVPDPDAVEIMKAG
                     PEIRYDGRFAPAGTNVNFARINQDSVYVRTYEKGVEAETFSCGTGVTATAIAASLRTG
                     RTDVRVVTKGGELRVHFSRKDNNFEGVFLEGPAVKVFSGSINL"
     gene            9046..9579
                     /locus_tag="PKI35_10455"
     CDS             9046..9579
                     /locus_tag="PKI35_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX44848.1"
                     /translation="MEEATISIRALEPTDIDLLYKWENDPEVWHVGNTIAPLSRFVLE
                     QYLVNSHEDIFSARQLRLMIEYNTAGADAVPVGSIDLFEFEPLHRRAGIGILIAKEHR
                     HKGYAQQALELMLSYCYNTLSLHQVFCNIEADNTESIRLFTKLGFVQCGYKKQWLWRK
                     QEWIDEIMYQHFDNQPL"
     gene            9613..>10573
                     /gene="mltG"
                     /locus_tag="PKI35_10460"
     CDS             9613..>10573
                     /gene="mltG"
                     /locus_tag="PKI35_10460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNX44849.1"
                     /translation="MVDYYSSLRKKEKKKARNRRLVFWLLTLLLLGGGTLAFLGYRAI
                     YSSNIWLNGQKEVSIIIPTGSDWSDVKQILYKNGTIVHRETFEWLAQYMKYPGHIKPG
                     HYLLKDGMNNRQLITKLRAGKQDPVKLIFNNIRTKEDLAGRIGGQLEPDSLKLITMLN
                     DTAVIGKWGFTPFTILTMFIPNTYEVYWNITADKFLERMHKEYNSFWNENRQKKLTET
                     GLTRLQAMTMASIVEKETNMNAEKPDIAGVYMNRLKQGWLLQADPTLVFALGDFTIKR
                     VLNVYKKINSPYNTYLYIGLPPGPICMPSISSIDAVLNYSHHNY"
BASE COUNT         2753 a         2463 c         2412 g         2945 t
ORIGIN      
        1 gcggcagaaa ccgcatcagc cggaaaatga ctaatttcgg gccatggaaa gattaagaat
       61 tacagccgtc aaatattaca acacgcttcc ttttatttat ggaattaccc gttccggatt
      121 gctgtccggt tttgacctga gcctggacgt gccttcggaa tgcgcccgca aactcgccgc
      181 cggtgaagcc gacctgggcc ttatccccgt gggggcgttg ccgtcaatcc ctgattacag
      241 gatcatcagc gatttttgta tcggcgccgt cggcgacgta cagtcggtta tcctgctgac
      301 ggattcggaa ttatcatccc ttacgaagat atacctggat accgacagcc gtacatcggt
      361 cgtactggtt aaagtgctgg ctgaacggtt atggaagata aacccggcct gggaaagcgc
      421 ctcaaacttc acgggcgccc tgaaacccgg cgaagggatc gtggctatcg gggataagac
      481 ttttagtttg cggccaaaat tccgttgttg ttatgacctg gccggcgaat ggatccggat
      541 gacgggattg ccttttgttt ttgccgtatg ggcttcgcgc aagcctttaa atgattcgtt
      601 cctccggagt ttaaacaatg ctctcggctg gggcgttgaa cataagaatg aatccgtctc
      661 catcgctgcc gatctgatca tcagcaagga agagctcctc tcctacctcg aacaggctat
      721 tcattatccc ctcgatgata aaaaacggca gggcatgaaa ttgtttcttt cgatgttata
      781 aaaaaaatca gccggctggc ggctgattta aaatttgttg tttcctgatc attttatctt
      841 ttcacattca cgccttgctt cttggccatt tcttcgaggc gtttctggaa agaggtcttt
      901 ttgaccggtt tggctttatt ttcctggatc cgtttgtgaa gcttttcttc gtccacaaaa
      961 cggcgtatca ggaacatctg tgcaaatgtg aaaacgttgg ccagcaggta gtaataactg
     1021 agtccggccg agtaattgtt gaagaatccg aggaacatga caggcatgag gtacatcatc
     1081 gctttcatgc cgggcatctg gttggtggag cccatcatgt cgttgttgat cttggtgtag
     1141 atgatggtgg agacggtcat cagcagggta aagaggctca catggtcgcc atagaacgga
     1201 atactaaacg gcaggttata gatgctgtcg taactggaaa ggtcgtgagc ccaaaggaat
     1261 gattgctgcc gcagctcgat ggatgccggg aagaagcgga acatggccac cagtataggg
     1321 aattgcagca gcatgggcac gcaccccgac atggggttaa cgcccgcttt tttatacaaa
     1381 gccatggttg cctgctgttt tttcatagcg tcttcctgct tcggatacct ggcgtttatt
     1441 tcgtcaacct ccggcttcag gacacgcatc ttggctgatg acatgtaggt tttataagca
     1501 atcgggaaca gcacaatttt aagcattatg gtcataatca ggatgatgat gccataattt
     1561 aaaccgtatt gctccagcca gttaaaaata agcaccacca cgtacttatt aaaccaggca
     1621 ataggcctga aactccatcc aagcggaatc tgccgttcaa gtttcaggtc atattttttt
     1681 aatgtacgga acttgttagg gccataatag cactgcatct tataatcctg gttctgtccg
     1741 ggatcatagg aaaccaggaa tgccgttttc atattcttca gcagccttcc gtttacatcc
     1801 tgataggacg tagtggcgat atcggcgctg ttgaaggcat cggatgctat catcgccgcc
     1861 gagaagaact gtgttttaaa ggacacccac ttcaccgccg atgtgatgtt ttctttcgaa
     1921 tcctttgttt ctttcaggta atcgaaatcc ccggtgttat ccatgaagta tatggtcgac
     1981 tggtcccttt cggttttaaa acctgcttcc tgccggagca tatccgtgct gacggaaagg
     2041 gcgatatccc gggtattgcg ggctataata ttctccattc ccaccatccg gatacggaat
     2101 ccgatcatgt attcatcctt gtatagcgta tacaggtact cgatataatg atcgccggcg
     2161 cccaggctat cggaagccgc atttaacctg aaagcgaact gcaccgaatc cttgccggca
     2221 atcgtaaggg aatctcccgg tgtagcggta acgggcgatg gaacgaaata gaggtcgccg
     2281 gtatttatgc ttttcaggtc ttcggtatta aattcaaggt caaaacggtt cgaatcgccc
     2341 tcaaacagta tgagcggaag cttgttataa gttttataat tttttaattc agccgaccaa
     2401 atgcgtccgc cccgggccga cagtttcagt ttcagcacct cattttccag ggtataaaac
     2461 ttctgaactc cggatgaggc tgaagcaaaa gtcccgtact gattactttt tacctgaaga
     2521 ttttcaaggg tatcgggttt gtttggtacg acgacggctg ccttttcaac agaatccttt
     2581 tcagccagca gttttgcctg tgcaatggag tctttgtact gaacaagggc aatggaatca
     2641 gctttgtgtc ttgctttttg gatatcctcc tgcgaaggcg acatccagat actatagcca
     2701 attaccatag caaaaatgac aatcagcccg ataatattac tcttactcat gaatcagatt
     2761 ttccttttaa agataagggg gcaaagataa aggaatttcc gatgccggat tcccctgatt
     2821 cctcattagc cggcaaggaa taaaatccgc aacaggaaat ccgggaagcc tgttatttac
     2881 tttgtttcgg ttttcttaaa ctgttctgct gcttttacaa aactaacgaa aagcggatgg
     2941 ggattagcaa ctgtgctgcg gtattcggga tggaactgga cgccgatgaa ccagggatga
     3001 tctttgagtt cgatcatctc caccaggtca ttctggcggt taatgccaac ggcaagcatc
     3061 cctgccttgt tatattcgtc gaggtacttg ttgttaaact cgtagcggtg acggtgccgt
     3121 tcggtgatgt cggcagtacc gtagatctca taagattttg agtcttccct gatggtgcac
     3181 ggataagcgc ccagccgcat ggttccgccc ttgttgctga tttttttctg ttcctccatg
     3241 atatcgatca ccggatacgg agtcttaggg ttcatttcgg tagagtgggc atcattcatt
     3301 ttcaggacgt ttcgtccgaa ttccaccacg gcgcattgca ttccgaggca tattcccagg
     3361 aaaggtattt tattttcccg ggcatatctg atagccagga tttttccttc tataccgcgc
     3421 tcgccaaaac cgggagccac cagaatccca tccaggtctt tcagcaggtg tttaacggtt
     3481 ccgctgttga tgctctccga atggatcatt ttcaccttta ccttcaactg attggcggcg
     3541 ccggcatgaa caaatgcctc gcttatcgac ttataggcat ccttgagttc aacatattta
     3601 ccgatcagcc caatctgtac ctcggcgacc ggatgtttca gcctgaacag gaattcttcc
     3661 catctcgcca ggtcgggttc tttccggatc gggacctgca ttttctccag aaccacctgg
     3721 tcaagctttt cgtcgcgcat gagcagggga acatcgtaga tggtttccgc atcaatcgat
     3781 tcgatgacgg ctgaagggtt cacattgcaa aacagggcta tcttattccg cagcgattgc
     3841 gtgagatgtt tctcggtacg gcaaacaata atatcaggct gcactcccga ttcaagcaag
     3901 gttttcaccg aatgctgcgt aggcttggtt tttagttctc ctgaagccgc caggtaagga
     3961 accagcgtca gatgaatcac gacgcagcgg tttcccattt cccatttcat ctggcgtacg
     4021 gcttcaatat aaggcagcga ttcaatatcc ccgaccgtcc ctcctatttc agtaatgacg
     4081 aaatcatatt ttccgctcag gcccagggtt tgtatacgtg atttgatctc gtcggtgata
     4141 tggggtatca cctgtaccgt ttcacccagg taatcgccac ggcgctcttt ttcaatgacc
     4201 gcctgataga tgcggccggt tgttacgtta ttggcctgcg atgtcggaac gttggtaaat
     4261 ctttcataat gcccgaggtc gagatcggtt tcggcgccgt cggtagttac gtaacattcg
     4321 ccatgttcat aagggttaag ggttcccgga tcaatattta tgtagggatc cagtttttgt
     4381 atcgtaaccc tgaaaccgcg ggaaataaga agtttagcga gagacgaaga gattattcct
     4441 ttcccgagtg aagaggccac gcctcctgtt gtgaaaatat attttggctc agacatgatg
     4501 ttgctgattt attgaatagt aatgtgttaa acaggccggt ttaaatcaat taaaatattc
     4561 cttcccggat tatccgaaag ttccgatgca ttcccaaaaa aattacatcc agcgaaatat
     4621 ttcatacgga gaggcgatgc aatcccgaca gaagattaga gctaactcaa aataaagcgc
     4681 ttaacggcgt ttgtctctat cattgcccgt tgtaatcccg gcagcaaaat tacaaatacc
     4741 cgcttgtatt acgaaggaaa aaaaatttat ttacgcgatt gaaaaattat ttttctgcga
     4801 tgattatcaa ccgataacag taacctttga attgctgaag acaaaaaaaa ccacccaaag
     4861 gtggtccgtt gtgatgaaac ccggataccg gttcttctat gcatatttca cggcataatg
     4921 catatttacc ggcaccagtt cggtgaccac tgaaaggatt tgttccttgg tcagaggttt
     4981 tgagagaaac ctgtccattc cggcttccag gcatttagcc ctggtaactt catctgtaaa
     5041 agccgtaacg gcaacaattg gtgttgaacg gtcaaagtgc ctgatacagg tagtcgcttc
     5101 aattccatcc atgccaggca tgtcaacatc catgaggacc acatcaaaaa cacctgtccg
     5161 gcaggcatca acggctccga aaccatcgct gacaatagtg taccgccagt cgaaacgttt
     5221 cataaagagc gtcataagct gctggttcaa atggttatct tcggcaataa gtacattcat
     5281 atcttttaca agtaaaacag gtacaatagc tgaacaaaga tttgtagtgt aatttaatcc
     5341 aaaaaccgct aaatggtaat acgttattaa caaatttttt ttcgatggtt aataaattaa
     5401 atatcaatac aatgaaatgc aatcacagca aaatgtgaaa aagtgttcaa ataaatatta
     5461 aaaaaaccac cttccggtgg ttttaaaaat aattcaggag ccggtaaaat ggccgattat
     5521 tgccccagat aagcctttaa taacttgctg cgggaattat gcttcagccg tctgatcgcc
     5581 ttctctttaa tttgcctgac gcgttcccgg gtaaggtcaa acctggcccc gatttcttcg
     5641 agggtaaggc catggggaat gccgatgccg taatacaggt tgatcacatc ccgttcttta
     5701 tcggacaagg ttgagagtga acgctgaatt tcctgggcaa gcgattcgcg catcaggccg
     5761 gcatccgtat agggagcatc ttcatcaaca aacacatcga tgaattttgt ctcttcatct
     5821 tcagccagcg gagcgtccat agagatgtat ttggtggaaa tctttaacgc tgcatcaatc
     5881 ttgtattcag ggatttcaag ggctgcggcg atttcgtctg ccgacggcgt acgctcaaat
     5941 ttttgttcca gccgggaggt ttctttcttg atcttattca gcgagccgac ctgattcagg
     6001 ggcagcctca caatacggga ttgttcggct aatgcctgca gtatgctttg cctgatccac
     6061 cacactgcat aagagatgaa tttaaacccg cgggtttcat caaaccgttg cgctgctttg
     6121 atcaatccga ggttaccttc gtttatgagg tcgggaaggc ttaacccctg gttttgatat
     6181 tgtttggcaa ccgaaacaac gaacctcagg tttgccctgc aaagcttatc cagtgcacgc
     6241 tcatcgccgg accttatttt acgggcaagt tccacttctt cttctgccga gataagccct
     6301 tccttgccga tatcctgcag atacttatca agcgaagctg tctccctgtt ggttattgat
     6361 ttcgatattt ttagctgtct catggatgct ggtaattgcg gagatatttt cagccttgaa
     6421 attttccgga atatctccct tgtctaacgt aaaattacgg aactaattgg caaaatccta
     6481 tttttattgt ttcttaaatt caataaacct gggatttcgc ctggattatt ttcagagtcc
     6541 aaacccataa taggcgcggg tccggttagg atatatgcct tcgatgagta caccgccttt
     6601 tttattttcg atggcatttt cgatgtcttt ggccgatttt accggttggt tatcaacttc
     6661 ggtaatgatg aagccaacct taatgccggc ttcttttaat ataccatcct gaagagcagt
     6721 aacctgaact ccgttggaaa tgcccagctt ttttctcatt tcgggcgata ctataccaaa
     6781 ggaagctcca agcctttcca cattatcctg ttcttcttta gcaataacag acgtagtacc
     6841 tcctttgttc ttgagggtaa cctgaaaatc tttttcagag ccctcgcgca ggaccgatac
     6901 tttcgcttta tcacccggac ggtattggcc gacctgttca agcagttcag aagtgctgtt
     6961 gacattaata ccgtcgatag ccgtgatcac atctcctttt ctgatgcctg cctcacttgc
     7021 cgaactatta tcggtaacat ctgagatata aacgccgcgc aactgcctga tattattttc
     7081 gccggcaaat ttgctgtcga tatcgcggat actgacgcca aggaaggcgc gttgaaccgc
     7141 accaaattca atcaggtcgg caaccacttt tttagcaata tttaccggga tcgcaaacga
     7201 atatcctgca taataacccg ttccggaagc gatagcagca tttattccta ccagttcgcc
     7261 acgggtgtta acaagggctc ccccactgtt gccgcggttt acggcagcat cggtctgtat
     7321 gtacgattct atggccgatc cgtcaggcat gccaagtata tctatgttcc tggccttggc
     7381 gctcacaatc ccggcggtca ccgttgaagt aagattgaac ggatttccaa cggccaacac
     7441 ccattcaccg attttcaggt catcggaatt accgtaaacg atatagggaa gatctttttc
     7501 atcaatctta acgacggcaa tgtcggtaga aggatcggtt cccagaattt tggcatcaaa
     7561 ttcacgttta tcattcagca ccacggtaac ggtcgtcgca tcctgaacta catgattatt
     7621 tgtcacgata taaccatcag gggaaacgat tacgcccgat cctgaacctt caagaggttg
     7681 ttgtatgccg ggctgacggt tattgaaaaa atcatgcaga tcgaaataat aatcgtaata
     7741 cgaatttttc cgctcatagg tcgtcttgat atggacaacc gcatgtatcg acatattggc
     7801 ggctgttgta aagtccggca gcaccgaacc gtcgcctgac atactggcat aagttacagg
     7861 aataggctgc tggggcacgc cctgcaattt ttctgacctt ttctgaatta gcgcataaga
     7921 acccagcgaa atgaaaccgc ccaaaattgc tatcataagg tagcctgcat actttttcat
     7981 cttttttctt ttagttaatt gttcaacaat gttgtaattc atttgtacct ctgattgttc
     8041 aatcattttt gaaagcaatg tattaacgca atattagtac cattcgtttt atttgtcatg
     8101 ttaatatttg ttaagagcaa ccagccctga aacgcctgat tattagcaat aaaagtccat
     8161 tttatggcct gccctcattt tagttgatac cctgtattct attttattac ttttgttagg
     8221 acggatgtcc ggattaccgg aaccattaat aatatggagc tactgcattt tgataaatac
     8281 cagggtaccg gcaacgattt cattcttgtt gacgacagga aacaacatcg tgattttaac
     8341 ggcaaaacca ttgtaaaact ttgtgaccgc cgctttggca tcggttccga cggattactg
     8401 cttttacgtg aaaggcaggg ttacgatttt gaaatgcttt tttttaatcc tgatggcagc
     8461 cgggcgacct tttgcggaaa cggggccagg tgtatctcag catttgcggc ttcgttgggc
     8521 gttatacagg atgaagcctc ttttcttgct cctgacgggg cgcacaaagc atcggtcctg
     8581 agccgcaacg gtaatgacag ccttgtaagc ataagcatga ttgaccctgt tatttaccat
     8641 cattccgaag attataccta cctgaattca gggacgtatc atgtagtgaa atttgttccc
     8701 gatcccgatg ccgttgaaat catgaaagcc ggaccggaaa taaggtatga cggacgtttt
     8761 gcaccggccg gaacgaatgt gaattttgcc aggatcaatc aggattcggt gtacgtaagg
     8821 acttatgaaa aaggtgttga agcggaaacc ttctcctgcg ggacgggcgt tacagccacg
     8881 gcaattgctg cttcgctcag aacagggagg acggatgtgc gggtggtgac caaaggcggt
     8941 gaactcaggg tgcatttttc aagaaaagat aataattttg aaggtgtttt cctggaaggc
     9001 cctgcggtga aagtattctc aggttcaatc aatctctgac caacaatgga agaggctaca
     9061 atatcaatca gggcgctgga acccacggat atcgacctgt tatacaaatg ggagaacgat
     9121 ccggaagtct ggcatgtcgg gaatacaatt gccccgttgt cccgctttgt gctggaacaa
     9181 taccttgtaa attcacatga agacatattc tctgcccgcc agctcaggct gatgattgaa
     9241 tataatacag ccggcgcgga tgccgtaccg gtggggagca tcgatctttt cgagtttgaa
     9301 cccctgcaca ggcgggccgg aatcggaatt ctgatagcca aagagcaccg gcataaagga
     9361 tatgcccaac aagcgctgga actcatgcta tcctattgtt acaataccct aagcctccac
     9421 caggttttct gtaacattga agccgataat acggaaagca tcaggctgtt taccaagctt
     9481 ggttttgtgc agtgcggtta taaaaaacaa tggctctggc gaaagcagga atggattgat
     9541 gaaattatgt atcagcactt cgataaccaa cctctatagc tcatctcatc attgcgatca
     9601 aacctcaaca ttatggttga ctactattca tcattgcgaa aaaaggaaaa gaaaaaagcc
     9661 cgcaaccgtc gcctggtttt ctggttactc acccttctgc tgcttggcgg cggcacgttg
     9721 gcattcctgg gatacagggc tatctacagt tccaatatct ggctgaacgg gcaaaaagaa
     9781 gtttcgatca ttatcccgac cggttctgac tggagcgatg tgaaacaaat actatataaa
     9841 aacgggacga tcgttcaccg tgaaactttt gaatggcttg cgcaatacat gaaatacccc
     9901 gggcatatca aaccagggca ctacctgctc aaagacggca tgaacaacag gcaactgatc
     9961 accaagctca gggccggcaa gcaggatccg gtaaagctca tcttcaataa catcaggact
    10021 aaagaagacc tggccgggag aattggcggg caacttgaac ccgattcgct gaaactgatt
    10081 acgatgctga acgacaccgc cgtcatcggt aaatggggat ttacaccttt taccatcctc
    10141 accatgttca ttcccaatac ttatgaagtc tactggaata ttacagccga taagttcctc
    10201 gaacggatgc acaaggagta caattccttt tggaacgaaa acagacagaa aaagttaacg
    10261 gaaacaggac taacccggtt gcaggcaatg acaatggctt cgatcgttga aaaagaaacc
    10321 aatatgaatg ctgagaagcc ggatattgca ggcgtttata tgaacaggct gaaacagggc
    10381 tggctgttgc aggccgaccc gacgctggtt tttgctttgg gagatttcac catcaaacgg
    10441 gtattgaatg tttataaaaa gatcaattcg ccatacaaca cctaccttta catcggtttg
    10501 cctcccggac caatctgtat gccttccatt tcatccatcg atgcggtcct gaactattcc
    10561 catcataatt aca
//
LOCUS       DAOPYV010000092        10176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_7701_length_10176_cov_12.520601, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000092 DAOPYV010000000
VERSION     DAOPYV010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10176
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7701_length_10176_cov_12.520601"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1913)
                     /locus_tag="PKI35_10465"
     CDS             complement(<1..1913)
                     /locus_tag="PKI35_10465"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX44850.1"
                     /translation="MNPFREIHNNRIWFIINATGLCAAMACIILVSLFVRRELSFDRF
                     HSKAGQIYRLTMNTNTGATAMHAARVQGNWPAEMARTYPEILKMARLIPSRRPVIRIG
                     DNVFMSEKVFATDSTFFEVFDFKVLAGNIKKSFRNPRGAFITRSIAEKYFGSTDVLGK
                     ELNILWHQDSQPYPYIIEGVMEDMPENSHFHADILVASPKQSDEGTWAFTYFLMKKGA
                     DIKNIEQKVRQKWIKEAKNNEPVPFIELQKLTDIHLHSHLTREIEQNGDMRSLILLMS
                     GAFIVLLITLVNYLNLNRVQFIRKLKTYKVKLINGATKLHLSREIIANSLYTSVFSAL
                     VALLIVYELNAALGLNLLTGKAGFITLIILGFILLLSALSVVPLITSAVSTDIAMKQK
                     LGGLYTFPLVLQFCLAIIAIASTFILSRQIAYINNLHPASQNDNMMVIRYNRWDASRQ
                     YDVFKSELIKKTPILDVTCALEEPGGDINDGFLFEMEGVQNNDNQPLNIFTVDSNFFS
                     FFNIHPLAGTTDFGSIPGIQWEQEALEANMYKENKIFPEKVAQFEKSLSKFRGKYVLN
                     MSALKLLGISKPEDAVGKRFRLKFWIPYLTPEGEIIGVVPDFHYTEIHKPERPLVIFA
                     NKSFSSNFIISFDS"
     gene            complement(2066..4417)
                     /locus_tag="PKI35_10470"
     CDS             complement(2066..4417)
                     /locus_tag="PKI35_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX44851.1"
                     /translation="MQKNFLKIALRYLWRYRTYSTLNFVCLSFALTCSVLAVLYILNA
                     FSYDKFHKNYDRLFSVEAYVTFFNGDQAPRPILSASLPPLLKQQAPEIEEVTRVAERT
                     CSFTAGERSFVHNGYFADDNFFSVFNFPIVRGNKNKPMSGQNSILISEPMALKFFETT
                     DCLGKTLIMTDGNRKEALRIGGVFGRIPHESTMQFDFVIPFSKFLADNSWANDPDAES
                     NRTWILLNRNTDKSQVEGKIKNLIRHRESSLNQELFLFPLKDKALFWYSQGKRVWRGM
                     NNVVIVGAIGFAILLIACFNFINLAVALNFKRYREAGIKKVSGSGRGPLIFQFIGETF
                     ILTLFSLVAAILLSNLLIPGLNSVFHQEIHLNLLSFKMLLFFLSVIIFTTLASGLLPS
                     LYLASANPLQVLKGKIAAGNSFSLFRQSLIVFQFTIPVILIICLMVIKTQDRYMRDYD
                     LGVEKDRLLVMENTENIQKHAEPVRTELLSIPGIDAVSFTNCVPARGFMPTNEVTWEG
                     RDDSEKQFFWCINTDFDYNKTVRIRMKEGRYFNRSFSADSMCYVINDVAAKLMKYKNP
                     VGSSITVEGRKGTVIGVFKDFHVVDLAGPIVPVIIRLHPSGRQIILVKFTPGNFTELL
                     ARIKAAGKRYDPDAEFNIQLYRDLPSYSNLNLPSGLVGLAFIIALLLACLGLFGLASF
                     TAGSRTKEIGIRKTNGATTFSVMQMLLGNYTKWLLIAVAIALPAAFIMAQIFLGRFHF
                     HTPMPVWAFLAGPALTFVVALFTVSWQSWRAATRNPVEALRYE"
     gene            complement(4467..6842)
                     /locus_tag="PKI35_10475"
     CDS             complement(4467..6842)
                     /locus_tag="PKI35_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX44852.1"
                     /translation="MNVLFFKMAFRHIVRNKLHSLINIIGFSIGIATALCLFLFIHHE
                     TGFDKFMPAAERTYRMIETIKIKDNTQVSGLFWYPLAQEVKSQVPSVESCCRVSEYIG
                     SGVKANGHQIRLNKIKAVDGNFLDFFGLNLVSGAKASALNSPDKIVLSEETASLLFGT
                     AACEGRQIEFLDKLFTVSGVVKQMPSNTHLSFDALVTNTFLESCRDYYLGWTGGVTFV
                     SYIRLMQGVSPAQVKGPINSILDQHVNIPLKGSGMTVAADLQKITDIHLHSDPGWYDI
                     DTNRSLSSLCLITLVGLILLLLAVFNYISLYIAQKTGKTKDMGILSVHGANRLNLKRQ
                     LFLEVLIVSFIAGILAWILFRVFTPFLNSSFNTSVSLKGNILPALIFIIALNIILSFI
                     ITFFAGRGFSTLNITGILKGEVAGGSKMTAGKVLVAVQFFVITVLLTAGLYIHRQYKF
                     LLNHDLGFDSKNVLVLSADKNLPSDKLAQFKQQLMEIPEISGVSLTTQPLGSGLTSNG
                     YMLPGEEEATMINIIYTDTSFLDLFNIKLTSGRNFSGDPKADSNSIIVNNALVKYAGW
                     KEPLGQDIRRNGRLRVIGTVNDFNFSPLSESVKPLIISCNPAIDGCGYSYVNILCRTQ
                     DMQRLTAKLKTAWEKSFPDAAVEIDFLDRQLAANYDTLVSMNRMITFFTLLVMLIACL
                     GLMGLTIYMVQWRTKEIGVRKTNGATIRQILLMLNVKFLKWIFVSMLLSFPVAWVLIN
                     QFRSSFAYQASVDIGLFLAAGALAMITALLTITHLSWKAASQNPVEALRYE"
     gene            complement(6898..7596)
                     /locus_tag="PKI35_10480"
     CDS             complement(6898..7596)
                     /locus_tag="PKI35_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX44853.1"
                     /translation="MIKTVNLSKVFRTEEIETLALNNINFNVKSGEFAAIMGPSGCGK
                     STLLNIVGLLDNPSSGEYYFDGQQVGGLKEVNRTSLRKGKIGFVFQSFNLIDELNVFE
                     NVELPLVYLKLKASERRQMVETVLDRMKISHRKKHFPQQLSGGQQQRVAIARAVVANP
                     KLILADEPTGNLDSKNGLEVMNLLTDLNREGTTIVMVTHSMHDAGFAHRVINLFDGQI
                     ITDNTHAVSAEPVW"
     gene            complement(7670..8035)
                     /locus_tag="PKI35_10485"
     CDS             complement(7670..8035)
                     /locus_tag="PKI35_10485"
                     /inference="COORDINATES: protein motif:HMM:NF008912.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX44854.1"
                     /translation="MINKIEKFEDLDVWQNAVNLSVKIYDIFKDNRDFGFRDQICRAS
                     VSIASNIAEGFERQYNKEFIHFLFIARGSAGELRTQIEIGNRLQYFIEPDYNSLIDDS
                     RKISAQLYKLIQVRKERFK"
     gene            complement(8347..9597)
                     /locus_tag="PKI35_10490"
     CDS             complement(8347..9597)
                     /locus_tag="PKI35_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX44855.1"
                     /translation="MDKPIVKKKGIKRKHIYWIVGGLLLIFLVYKSVTGMRGSSYRAE
                     RDKLTISTVDSGLFNDYISVIGQVEPISTVFLDAEEGGKVVQILVEEGEMVKTGDVIL
                     RLKNNDLTLNIMNSESNLAYHSNELRNTRIQMEQQKIANRQQLNQIEYELNRLERKYK
                     QQKDLYIDQLIPREDFLQAEEDFEYKKQERELIYKKLIQDSIFRENQKRSMDENLSNM
                     QANLAMVQQRREDLDVKAPVDGQLGLLKAQIGESIGKGQRIGQINVLTSYKITAMVDE
                     HYIDRFRAGLPATIERKDDTYGLLVKKVYPEVRNGQFQIDLVFKGDVPENLRTGQTFH
                     LRIELGESEKATMVPRGGFFQSTGGQWIFVLDATGREARKRNIQVGRQNPMYFEILDG
                     LKPGEQVITSGYELFGDNDRIVLK"
BASE COUNT         2580 a         2396 c         2260 g         2940 t
ORIGIN      
        1 gaatcaaaac ttatgataaa attggaactg aatgatttat tggcaaatat aaccagcggc
       61 ctttcaggct tatgtatttc ggtataatga aaatctggca ccacaccgat tatctcacct
      121 tcgggagtga ggtacggaat ccagaatttc agacggaagc gttttccgac ggcatcttcg
      181 ggttttgaga tacccaacag cttaagtgcg ctcatgttta agacatattt tcccctgaat
      241 ttggacagtg atttttcaaa ttgtgccact ttttcgggaa aaattttgtt ttccttatac
      301 atattcgctt ctaaagcctc ctgttcccat tgtatgccgg gaatactgcc aaaatccgtt
      361 gtgccggcaa gcggatgaat attgaaaaaa ctgaagaaat ttgaatcaac tgtaaaaata
      421 ttaagcggct gattatcatt gttttgaacg ccttccatct caaacagaaa tccgtcgttg
      481 atatcaccgc cgggttcttc gagggcacag gtaacatcca gaatgggcgt ttttttaatt
      541 aattcgcttt taaaaacatc atattgcctt gaggcatccc agcggttgta tcggatcacc
      601 atcatattgt cattttggga tgccgggtgc aggttattga tatacgctat ttgtcgcgac
      661 agaatgaaag tcgacgctat cgctatgatg gccagacaaa actgcaacac cagcggaaaa
      721 gtatacaaac cgcccagttt ctgtttcatg gcgatatctg tgctcactgc tgaagtaatc
      781 aggggaacta ctgacagcgc tgataataag agtataaagc caagtattat cagggttatg
      841 aatcccgctt ttccggtcag caggttcagc ccgagggccg cattaagttc gtaaacaata
      901 agcaatgcaa ctaaagccga aaacaccgaa gtatataatg aattggcaat tatttcacgg
      961 ctcaggtgca gtttcgttgc tccgttgata agtttcacct tataggtctt cagctttcgg
     1021 atgaattgaa cacggttcag gttgaggtag ttgaccagtg tgatgagcag tacaataaaa
     1081 gccccgctca ttaacaaaat caatgaacgc atgtcgccgt tctgctcaat ttcgcgggta
     1141 aggtggctat gcagatgaat atccgtgagt ttctgcaact caataaaggg aaccggctca
     1201 ttgtttttgg cttcttttat ccatttttga cggacttttt gttcgatatt ttttatatca
     1261 gccccttttt tcattaaaaa ataggtgaaa gcccatgttc cttcgtcact ttgttttgga
     1321 gatgcaacaa ggatatcggc atgaaaatga gaattttcag gcatgtcttc catcacccct
     1381 tcgattatat agggataagg ctgactgtcc tgatgccata aaatgtttag ttccttaccg
     1441 aggacatcgg tgctgccaaa atatttttcg gctatacttc gggtgatgaa ggcgcccctc
     1501 ggattcctga aacttttttt aatattgccg gccagcacct taaaatcaaa gacctcaaaa
     1561 aaagtactgt cggttgcaaa tactttttcc gacataaata cattatcacc aatcctgatg
     1621 acaggacgcc gtgacggtat cagcctggcc atttttaaaa tttcaggata tgtacgggcc
     1681 atttcggccg gccaattgcc ttgtacgcgg gcggcatgca tcgcggttgc ccctgtattg
     1741 gtgttcatcg taagccggta aatctgaccg gctttggaat ggaacctgtc aaaactcaat
     1801 tccctgcgta caaaaaggga aaccagaatg atgcaggcca ttgccgcaca aaggccagtg
     1861 gcgttgatga taaaccatat ccggttgtta tggatttctc tgaaagggtt catgaaatat
     1921 tttcagtttt tatagatatt ttcagttttt gggtctctga ttaaactcat cgccaaacct
     1981 tcagcgtatt ctgattgcaa tccattttta acctgcctga atcgttcgca ttctgatttt
     2041 tcatttatca ttcaccgttc agactttatt cgtacctcag cgcttccacc gggttacggg
     2101 tagcggcccg ccaactttgc cagcttacag tgaaaagcgc cacaacgaag gttaacgccg
     2161 gtccggccag gaatgcccat accggcatcg gggtgtggaa gtggaacctt cctagaaata
     2221 tttgcgccat tataaaagcg gccggcagcg caatagcgac agcaataagc agccatttgg
     2281 tataattgcc aagcagcatc tgcatcaccg agaatgttgt cgccccgttg gttttccgga
     2341 tcccgatctc cttggtgcgg cttccggctg taaacgatgc cagcccgaac aatcccagac
     2401 aagccagtag gagcgcaata ataaaagcga gtccaaccag tccggacggc aaattcaggt
     2461 ttgaatatga aggcaggtca cggtacaatt gtatattgaa ttccgcatcg gggtcatagc
     2521 gtttgccggc cgcttttatc ctggccagca attccgtaaa gtttccgggg gtaaatttga
     2581 caagaatgat ctgccggccc gaaggatgca gacgaataat caccggaaca atcggtccgg
     2641 cgaggtcaac tacatgaaaa tctttgaaaa ccccgatcac ggttcctttc ctgccttcaa
     2701 ctgttattga cgagccgacc ggatttttgt atttcattaa tttggcagct acatcgttga
     2761 taacatagca cattgaatcg gcagaaaatg accggttgaa gtaccggcct tctttcattc
     2821 tgattctgac cgttttatta taatcaaagt cggtattgat acaccagaaa aactgttttt
     2881 ccgaatcatc cctgccttcc cacgtcacct cattcgtcgg cataaaacca cgggccggca
     2941 cacaattggt aaaactcacc gcgtcaattc ccggaattga taaaagctcc gttctgacag
     3001 gctccgcatg cttttgaata ttttcggtat tttccatcac aagcaacctg tctttttcaa
     3061 ctccaagatc ataatcacgc atataccgat cctgggtttt gataaccatc aaacagatga
     3121 tcaggatcac cggtatcgta aactgaaata cgatgaggct ttgcctgaat aagctaaagc
     3181 tgttgccggc agcaattttc cctttcaata cctgaagtgg atttgccgat gccaggtaga
     3241 gcgaaggcag caagcccgac gccagcgtag taaagattat cacagacagg aaaaataaaa
     3301 gcattttaaa ggaaaggaga ttcagatgaa tttcctgatg aaaaaccgaa ttgagcccgg
     3361 gaattaaaag gtttgacaac agaatcgcgg caaccaggct gaaaagcgtc agtatgaacg
     3421 tttctccgat aaactggaag atcagcgggc ctcttcccga gcccgacact tttttaatac
     3481 cagcttcgcg gtaccgctta aagttgagcg caacagccag gttaataaaa ttgaagcagg
     3541 ctatcagcag gatggcaaag ccaatggcgc ccacaatcac gacattattc atcccgcgcc
     3601 acaccctttt tccctgcgaa taccaaaaga gcgctttgtc tttcagcggg aaaaggaaca
     3661 gttcctggtt cagagacgat tcgcggtgcc ggatcaggtt tttgattttt ccctccacct
     3721 ggcttttgtc cgtattccgg ttcaggagaa tccaggtgcg gtttgactca gcatcaggat
     3781 catttgccca actattgtcg gccaggaatt tcgagaacgg gatcacaaaa tcgaactgca
     3841 tggtggattc atgcggtatt ctaccaaaga caccgccgat ccgtaatgcc tcttttctat
     3901 tgccatcggt cattatcagg gttttaccga gacaatccgt agtttcgaaa aattttaaag
     3961 ccatcggctc cgaaatcagg attgaattct ggccgctcat cggcttgttt ttgttccctc
     4021 tgacgatcgg aaagttgaaa acgctaaaaa agttatcgtc agcaaaataa ccgttatgaa
     4081 caaatgatct ttctcctgcc gtaaaagagc aggtgcgttc ggccaccctg gttacttcct
     4141 caatttcagg agcctgctgt ttgagcaatg ggggcagcga agccgacagg attggcctgg
     4201 gagcctggtc gccgttaaaa aaagtcacat aagcctcaac ggagaaaagc cggtcgtagt
     4261 ttttatgaaa tttgtcgtag ctgaatgcat ttaaaatata aagcaccgcc aggaccgaac
     4321 aggtgagtgc gaatgaaagg caaacgaaat tgagcgtact gtatgtccgg tatctccaca
     4381 ggtatcgtaa agcaattttc aaaaagtttt tttgcatcgc ttatcttgat tattaccgtt
     4441 tcaaattaca ggttcacatt ttaattctac tcatacctca gcgcttcaac cggattctgt
     4501 gatgccgctt tccacgagag gtgcgtaatt gtcagtaatg cagtgatcat tgccagggcg
     4561 cctgctgcca aaaataaccc tatatcaact gatgcctgat aggcaaatga gcttctgaat
     4621 tgatttatca gcacccacgc aaccgggaaa gaaaggagca tagacacaaa aatccacttt
     4681 aagaatttga cattcagcat gaggagtatt tgcctgattg ttgcgccatt cgtcttccgg
     4741 acgccgattt ccttggtccg ccactgaacc atgtatatgg tgagccccat cagacccagg
     4801 caggcaatca gcatgaccag cagtgtgaaa aaggtgatca tccggttcat ggaaaccagt
     4861 gtatcatagt ttgcggccag ttgccggtca agaaaatcga tttcaacggc tgcatcggga
     4921 aaactttttt cccacgctgt tttcagtttt gctgtcagac gctgcatatc ctgcgtcctg
     4981 cagagaatat taacatagct gtagccacat ccatcaatgg cgggattgca actgatgata
     5041 aggggtttga cgctttcgct gagcggcgaa aaattgaaat cgttcacggt gccgatcacc
     5101 ctgaggcggc cgtttctcct gatatcctga cccagcggct ctttccaccc ggcatatttt
     5161 accagggcat tgttgacgat gatggaattg ctgtcggctt tcgggtcacc gctgaaattt
     5221 cttcctgaag taagctttat attgaacaaa tccaggaagg atgtatccgt gtaaatgata
     5281 ttgatcatag tagcctcttc ctcaccggga agcatatagc cgttggatgt caatccgcta
     5341 cccaggggct gggttgtaag cgaaacccct gatatttcag gaatttccat gagttgctgc
     5401 ttaaactgtg caagcttatc ggaaggcagg tttttatcgg ctgaaagcac cagcacattt
     5461 ttcgaatcga accccagatc gtggttgagc aggaatttat attgcctgtg gatgtaaagc
     5521 ccggcagtga gcaatacggt tatcacgaaa aactgaaccg caacgaggac tttccctgcc
     5581 gtcatcttac tgcctccggc aacttcacct ttcagaatac cggtgatatt cagcgtggaa
     5641 aatccccttc ctgcaaagaa ggtaataatg aaggataaga ttatgtttaa agcgataatg
     5701 aaaattaacg ccggcaatat attccccttc agggaaacac tggtattgaa gctcgaattg
     5761 aggaacggcg tgaaaacgcg gaacaggatc caggcaagga tgccggcaat gaacgaaacg
     5821 atcagcactt ccagaaataa ttgccgcttc aggtttaacc tgttggctcc gtgaaccgat
     5881 aatatcccca tatcttttgt ttttcccgtt ttttgggcga tgtacaggct gatgtagtta
     5941 aataccgcca gcaggagtaa aatcaggcca accaaagtaa tcaggcacag ggaactcagg
     6001 ctcctgttgg tatcgatatc gtaccagccc ggatcgctgt gcagatgtat atcggtaatc
     6061 ttctgcaagt cagcggctac tgtcattccg ctgcctttga gcggaatatt cacatgctgg
     6121 tccaatatgc tgttaatcgg ccctttgacc tgcgccggcg atacgccctg catcagtctg
     6181 atgtacgaca caaaagttac gccccctgtc cagccgaggt aataatccct gcaactttcg
     6241 agaaaggtgt tggttaccag tgcatcaaag gaaagatggg tattggatgg catttgttta
     6301 accacaccgg aaaccgtaaa cagcttatcc agaaattcga tctgcctgcc ttcgcatgct
     6361 gcggttccga ataacaggct ggcggtttct tcggagagca cgattttatc cggtgaattc
     6421 agagcgctgg ctttggcccc cgaaaccagg ttgagtccga agaaatcaag gaagttgccg
     6481 tcaaccgctt taattttatt caaccggatc tgatgaccgt tcgcctttac tccggaaccg
     6541 atatattccg aaaccctgca acagctctca accgaaggca cctggctttt gacttcctgc
     6601 gccagcggat accagaaaag ccctgaaact tgcgtgttat cctttatctt tattgtttct
     6661 atcatccggt aagtacgttc agctgccggc ataaacttgt cgaagcccgt ctcatgatgt
     6721 ataaacagaa aaaggcacag ggctgtggcg ataccgatgg aaaaaccgat gatattgatc
     6781 agggaatgca atttgttcct gacaatatga cggaaagcca tcttaaaaaa cagtacgttc
     6841 attacagctt tagcattttg aatcggaatt gattttcctg gttgtcagat ccacaggtta
     6901 ccacaccggt tctgcagaaa cagcgtgggt attatcggtt atgatctgtc cgtcgaaaag
     6961 gttgatgacc ctgtgcgcaa aacctgcgtc gtgcatggaa tgggtaacca tgacaatggt
     7021 ggtgccttca cggttcaggt cggtgagcag gttcatcact tccagcccgt ttttggaatc
     7081 gaggttcccg gtaggctcat cggcaaggat cagtttcgga ttggcgacca cggccctggc
     7141 gatagccaca cgttgctgct gaccgccgga gagttgctgc gggaaatgtt ttttccggtg
     7201 gctgattttc atacggtcga gaacggtttc aaccatctgc ctgcgttccg aagccttcag
     7261 cttcaggtaa accaggggca gttcaacgtt ctcgaacaca ttgagctcat caatcaggtt
     7321 aaaactttgg aaaacgaagc ctattttgcc tttacgaagc gaggtccggt ttacttcttt
     7381 caacccgcca acctgctgcc cgtcgaaata atattcgccg gatgaagggt tgtcgagcaa
     7441 cccgacaata ttcagcaacg tggacttacc gcaacccgaa ggacccataa tggcggcgaa
     7501 ttcaccactt ttaacgttga aattgatgtt gttaagcgcc aaagtttcga tttcttcagt
     7561 ccgaaatact tttgagaggt ttactgtttt tatcatggct gtagctatta gtgatcagag
     7621 aacggagaac ggagaatgga ggacggagag cgggaaatgg gtaacatttc tatttgaaac
     7681 gttctttcct gacttgtatc agtttataaa gttgggctga gatttttctg gagtcatcaa
     7741 tcagggaatt ataatccggt tctataaaat attgaagtct gttaccaatt tctatttgcg
     7801 tccttaattc tcctgctgaa cctctggcaa taaacaagaa gtggataaac tccttgttat
     7861 attgtcgctc gaagccttct gcaatatttg aagcaataga aacagaagct ctgcatatct
     7921 gatcgcgaaa tccaaaatca cggttatctt taaaaatgtc ataaatctta acgcttagat
     7981 ttacagcatt ttgccataca tccagatctt caaatttttc tattttattg atcattattt
     8041 cttagtcatt tgattatcaa ttcgctgcaa tacactggct gctaaataca tccagtttta
     8101 attcagaagt cagatcggtc gttcaatcac cttcatacct gactcatgaa cgcttatcag
     8161 gtatttcttc cttcttacgt attccgtatt ccgtattccg tattccgtat tccgtacttc
     8221 gtaatccgtt ctccgttttc cgttttccgt tctccgtttt ccgttctctg ttttccgttt
     8281 tccgttttcc gttctccgtt ttccgttctc cgttttccgt attccgtttt ccgtattccg
     8341 tcctccttac ttcagaacta tcctgtcatt atccccaaac agctcatatc cgcttgtaat
     8401 tacctgctct ccgggcttta atccatcaag aatttcaaaa tacatcgggt tctgcctgcc
     8461 gacctgaata ttgcgtttgc gggcttcccg acccgtagcg tcaagaacaa aaatccattg
     8521 tccgccggtg ctttggaaga aaccgcccct tgggaccatt gtcgcttttt cagattcacc
     8581 cagctcaatt ctcaggtgaa aggtttgtcc tgtacgcaga ttttcaggaa catctccttt
     8641 aaaaaccagg tcaatctgaa actgaccgtt acggacttcc gggtatactt ttttcaccag
     8701 caggccgtag gtatcatcct tacgctcgat ggtcgccggc aatccggccc tgaacctgtc
     8761 aatgtaatgt tcatcgacca tggcggtgat tttgtacgac gtcagcacat tgatctgccc
     8821 gatgcgttgc cctttcccga tagattcccc gatctgggct ttgagcaggc caagctggcc
     8881 gtcaacaggc gctttcacgt ccagatcttc gcggcgttgc tgaaccatag caaggttggc
     8941 ctgcatattc gaaaggtttt catccataga ccgtttttgg ttctcccgga agatggaatc
     9001 ctgaatcagt ttcttgtaaa tcagctccct ttcctgtttc ttatattcaa aatcttcttc
     9061 ggcctgaagg aaatcttccc ggggaatcag ctggtcgata tacaagtcct tttgctgttt
     9121 gtatttccgt tccaaacgat tcaattcata ctcaatctgg ttcaactgct gacgattggc
     9181 tattttttgt tgctccatct gaatgcgtgt gtttcgcaat tcgttggaat gataggcgag
     9241 gttcgactcg ctgttcatga tattcaaggt taagtcattg ttcttcaagc gaagaatcac
     9301 atctcccgtt ttcaccattt caccctcttc aacgagtatt tgaactacct tgccgccttc
     9361 ttcggcatca aggaaaacgg tggaaatggg ttccacctga ccgataaccg aaatataatc
     9421 attgaatagt cccgaatcaa ccgtgcttat tgttagctta tcccgctcag cccggtaaga
     9481 agagcccctc atccccgtca ctgatttata aaccaggaat atcagcaaca atccaccgac
     9541 tatccagtaa atatgtttgc gttttatccc ctttttcttt acaattggtt tatccattgt
     9601 catagatgtg ctattttatg ctttgcttta gtatgggctt ttggctatta gcctgccgtc
     9661 ttacgctcag cctgcccagg caggtaaact gttagccctt ggcctttgac gttttgagtt
     9721 ctgtgttctg cgttttgcgt ttcgtgtttg cgttctgcgt ttcgtgtttg cgttctgcgt
     9781 tttgcgtttc gtggaattcg taattgacat tcgtggaatt cgtggttcgt cattcgtggg
     9841 cagggttcca gggtttcaat gtatcaatgt ttcagtgttc cagtgtttca gagttctgcg
     9901 ttttgtgttc tgcgttctgt gtttcagtgt ttcagggttt cagtgtttca ggcctgcctg
     9961 cccgggaagg ctgtgcgtaa ggtggcaggc ctgcctgccc gggcgaggtt gcgggtgagg
    10021 ggcagggttt cagcatttca ggttttgttt agccgttggc cgttagcttt tagcgttttg
    10081 agttttgagt tctgcgttct gcgttttgcg ttttgcgttc ttctcctttc tccttgctcc
    10141 ctactccctg ctccgttctc cctgtctcca ttttcc
//
LOCUS       DAOPYV010000093        10095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_7799_length_10095_cov_11.730876, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000093 DAOPYV010000000
VERSION     DAOPYV010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10095
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7799_length_10095_cov_11.730876"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1404
                     /locus_tag="PKI35_10495"
     CDS             169..1404
                     /locus_tag="PKI35_10495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX44856.1"
                     /translation="MQQIQYKVYGYRWVMLAIYMLIVAMNQLLWITFAPVTSEAARYY
                     QVGDLQIGMLSMCFMVVFILVSIPASWIIDSYGIRTGVGIGAALTGAFGLIRGFAGPH
                     YNILLVAQIGIAVGQPFLLNATTKIAARWFPMAERATAAGLGTLSMYIGILAGMIATP
                     YLVMGSGMASALYIYGIISLISAVLFMIFAKEAPPSPPCLSGQEERALVYDGLKQTLK
                     TRDFNWLMLIFFIGLGVFNCVTTWIENILSPRGFTTAQAGITGGLMIAGGIIGALVIP
                     LLSDHYRRRTPFIIFALTGATLGLAGITFAHNYYILLLSGAALGFFLLSSGPIGFQYG
                     AEITYPASEGTSNGLLLLMGQISGIAMILGMDMFKSPVNGSMFRPMIFLIIIMFMRIL
                     ISLKLKESVLMKQQHDKNE"
     gene            1417..2178
                     /locus_tag="PKI35_10500"
     CDS             1417..2178
                     /locus_tag="PKI35_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44857.1"
                     /translation="MYPSLRHILLKNHPAFGIAVKTIVLILGFLILMSRLSAQDTEYD
                     TTIRKKLDAAGLLLQKDANRTNAWWWGWLGGYSAATIGQVIIGVSAEKLNTRQDMFLG
                     AATTLVGAAGQFITPVSPSKAQLRILEMPERTLAEKKEKLAAAEEFLQKRSWSEIKGR
                     QWQMHAASGAVNLGSGLVTWLGFKRSWKDGLLNFALNTAITEAQIWSQPMTARNGLIR
                     YQKDYLPEKTALSGKHEMKWALYTGINNLGIRISF"
     gene            2175..2708
                     /locus_tag="PKI35_10505"
     CDS             2175..2708
                     /locus_tag="PKI35_10505"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNX44858.1"
                     /translation="MNDEELYICRWTHMDCVRGIDFLFSRPEVDTTRVAVEGASQGGA
                     LTFATAALNNTRIKVCVPQVPFLSDFEHYFRVASWPGNEFINYVEKEKKMTWEQVYNT
                     LSYIDIKNLAGWIKAPMLMGAGLVDDVCPPHINFAAYNQVKSGKHYIVYPLSGHGLPE
                     DFHFRKMDWIRSHFGLK"
     gene            2789..5173
                     /gene="galB"
                     /locus_tag="PKI35_10510"
     CDS             2789..5173
                     /gene="galB"
                     /locus_tag="PKI35_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalB"
                     /protein_id="HNX44859.1"
                     /translation="MTMIFSHSMAASDVRARDNFNQGWRFYPGDMPEARNYNFDDSQW
                     RQLDLPHDWSIEGDFSDQNPATPGGGALPGGIGWYRKTFKIPAADSQKLIYIEFDGVY
                     RNSKVWINGTCLGKRPYGYSSFRYELTPYLNFGDKPNVIAVKVDNSQQPNSRWYSGSG
                     IYRNVWLVKTGKIAVDHWGTYVTTPKIDQKGAEVFVKIRVRNSPGTMPAISIKSGIYN
                     QQNELVAEVITPKVKLEKGNTEIQQKLVVKNPVLWSDEKPEMYRLVTTLYTKSGILDE
                     YETPFGIRTFEFDAVRGFILNGRPVKIKGVCNHHDLGCLGAAINKRGLERQLELLKEM
                     GCNGIRTSHNPPAPELLDLCDRMGFIVMDEAFDMWKMEKTPCDYSRDFDEWHQRDIQD
                     MVYRDRNHPSVFIWSIGNEIPEQWDSTGFRLARELAGLVRELDKTRPITSACNNPDTS
                     NYIIKSGVLDLIGQNYHHGTFANFPTIYPGKKFIASETTSAIATRGSYDMPADSVRVW
                     PRYWDKPFTDGNADLSCSAYDNCRVPWGSTHEETWKIIKKYDYLSGLFIWTGFDYIGE
                     PTPYPWPARSSYFGILDLCGFPKDAFYMYKSEWTSTPVLHLLPHWNWKAGQDVDVWAY
                     SNCEEVELFLNGRSLGTQKKTGDQIHFAWKVPFEPGTLRAIGRTNGKQVLTDEVRTAG
                     LPARIILTPDRSSIHADGQDLSYITVQVADQNNIPVPDAGNRIYFSVEGDAAIAGTDN
                     GDPTSMESFKSHEKRAFYGRCLLVVKAGNKEGTVKITAVSDGLQSATENIQLVK"
     gene            5179..6381
                     /locus_tag="PKI35_10515"
     CDS             5179..6381
                     /locus_tag="PKI35_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HNX44860.1"
                     /translation="MKKLLFLAFVIAVQSAYCQKFDLAKTPPMGWNSWNTFDCNIDEN
                     LIRQTADAMVANGMKDAGYVYINLDDCWHGERDSLGFIHEDPVRFPSGMKALADYVHS
                     KGLKIGIYSDAGTQTCGGRPGSRGHEYQDAVTYANWGIDYLKYDWCSTGELNAKEAYI
                     TMRDAIHAAGRPMVFSICEWGSNKPWTWAKEVGHLWRTTGDIHNCWDCEMGFGTWSKL
                     GVLSILDKQNDLRKYAGPGHWNDPDMLEVGNGMSYTEDRAHFSLWCMLAAPLISGNDL
                     RNMPKETLDILTNKDAIAINQDELGVQCYKMLDMGNYEIYAKPLSNNELALCFLNRGE
                     TPWNIDFNWKNYPIYDGISGKGYDFVKTKFKIHDCWQKKDTGTTAEPFKPVLQPHDVI
                     LIRLTPVK"
     gene            7356..9560
                     /locus_tag="PKI35_10520"
     CDS             7356..9560
                     /locus_tag="PKI35_10520"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNX44861.1"
                     /translation="MSEIKKESFPVTGMACAACAVSVQSMLGSRKGVVSARVNYGNRT
                     VRLEYDPSIVAIGELRNLVQSIGYDMITEEENRSGKLEIVEHKRKLILRNRLIIAALF
                     SVPVFLLSMFFHNILPYQHYILLILSLPVILYSGAEFYPAAIRQAKHGIFTMDSLVST
                     GTGAAFLLSIFNTLFPEVLQSQGMEAYVYYESAVIIITFILTGRYIEENVKARASSAI
                     RKLTGLQPKKIMIRREGELFEIPAVHVIPGDVVTVRLGDRIPVDGTVVTGSASVDESA
                     LTGEPMPVAKQPASQVYAGTMNIQGLIEVEARKPGNETILSQIISLVDEAQSSKPPIQ
                     QYVDRIAGVFVPAVFIIAAITFLSWLFIGHSISHAFVTTISVLVIACPCALGLATPMA
                     LIAGIGRGASRGMIIRNARSLEDAAGIDVVVLDKTGTITSGKPSVSIRWLTETGDLPF
                     LLYCAEKQSAHPLAAAICNYLESSFELWGRPPLVFESFRETAGKGIAVVWNGTKFLIG
                     SEQYLLSENVKIEKGTEDSGKSNPAIYVAIESQPAATVEITDKLKPSSKKAVERLRAL
                     GIHTVLASGDSAKPAQTIASEAGVDKVYHSILPDRKGEIIKELQSEGHKVAMIGDGIN
                     DAYALAQADLGIAMGNGTDVAIESAGIILMKPELPLAVSAIRLSKWVVRLIRQNLFWA
                     FFYNVIAIPLAAGLLYPVNGFLLSPMVAGAAMAMSSLTVVTNSLRLKYVKIE"
BASE COUNT         2783 a         2569 c         2408 g         2335 t
ORIGIN      
        1 tttttacaat tcccggacaa tattttacag catctgctcc tgtccggcaa aatattatct
       61 gaaaaaaaat ctattttcgc acctgtcaag gacaatccat cttccttgat aaaatgtgat
      121 agcagaaacc tgcttcctga tttgtgtcat cctgacaaaa caaacacaat gcaacaaata
      181 cagtataaag tgtatggcta tcgctgggtt atgctggcga tatacatgct gattgtggct
      241 atgaaccaac tgctttggat tactttcgcc cctgtcacct ccgaagctgc ccgatattat
      301 caggtaggcg atttacagat agggatgcta tccatgtgtt tcatggttgt attcatcctc
      361 gtttccatac ctgcctcatg gattattgac tcctacggta tccgcaccgg cgtaggcatc
      421 ggcgcagcct taacaggagc cttcgggctc atccgtggtt ttgccggccc acattataac
      481 atcctcctgg ttgcccagat cgggatagcg gttgggcagc ctttcctgct gaacgccacc
      541 accaaaattg cagcccgttg gtttcctatg gcagaaagag caacagccgc cggattggga
      601 accttgtcga tgtatatcgg gattctggcc ggtatgattg cgacgcccta cctggtgatg
      661 ggatccggca tggcatcagc cttatatata tacggtatta tttccctgat ttccgccgtc
      721 ctgtttatga tcttcgccaa agaagccccc ccttcccctc catgcctttc cggacaggaa
      781 gaacgcgccc tggtgtatga cgggctgaaa caaacgttga aaacaaggga ttttaactgg
      841 ctgatgctga tatttttcat cggcctgggc gttttcaact gcgtgacaac ctggatcgaa
      901 aacatcctca gcccccgcgg gttcacaaca gcccaggccg gtattaccgg cggactgatg
      961 atagcaggag gaatcatagg cgccctggta ataccgcttc tttcggatca ttaccgcaga
     1021 aggacgcctt tcatcatctt tgcactgacc ggcgccaccc ttggactggc cggaattacc
     1081 ttcgcccata attattatat ccttttactt tcaggcgccg ccctgggttt tttcctcctg
     1141 agttccggcc ccatcggctt ccagtatggc gccgaaatca cataccctgc ttcggaaggc
     1201 acttcgaatg gattgcttct tctgatggga caaatttcgg gcattgccat gatcctgggc
     1261 atggatatgt ttaagtcgcc ggtcaacggt tccatgttca ggcccatgat attcctgatc
     1321 atcatcatgt ttatgagaat tctcatatcc ctgaaactga aagaatcggt cctgatgaag
     1381 cagcagcatg ataaaaatga ataatttcag gtgttgatgt atccttcgct ccgacatatc
     1441 ctgctgaaga atcacccggc ctttggcata gccgtaaaaa ccattgtcct tatactgggt
     1501 tttcttatcc tgatgtcccg gctatcagcg caggatacag aatatgacac caccatccgg
     1561 aaaaagcttg atgccgccgg gcttctgttg cagaaagatg ccaacaggac caacgcctgg
     1621 tggtggggct ggctgggagg atacagcgcc gcaaccattg gacaggtcat tatcggcgtt
     1681 tcagccgaaa aattgaatac gcggcaggac atgtttctcg gcgcggcaac aaccctggtt
     1741 ggcgctgccg gacagttcat tactcccgtt tcaccttcaa aagcacaact gagaatactg
     1801 gaaatgccgg aaagaactct tgctgagaaa aaggagaaac tggctgccgc tgaagaattc
     1861 cttcagaaac ggtcatggtc ggaaattaaa ggaagacaat ggcagatgca tgccgcttcg
     1921 ggcgcagtga accttggcag cggacttgtt acctggctgg gtttcaaacg aagctggaag
     1981 gacggccttt taaactttgc actcaatact gcaataaccg aagcccagat ctggagccag
     2041 cccatgactg cccggaatgg tctgatcagg tatcaaaaag attatctgcc tgaaaaaacg
     2101 gccctctcag gaaaacatga gatgaaatgg gctttatata cagggattaa taacctggga
     2161 atcaggatta gtttttgaat gacgaagaac tttacatctg ccgctggaca cacatggact
     2221 gcgtgcgtgg aatcgacttc ctgttttcac gtcccgaagt ggataccacc cgcgttgccg
     2281 tggaaggcgc cagccaggga ggagccctta cttttgccac cgccgctctg aacaataccc
     2341 gtataaaggt ctgtgtgcca caggtaccat tcctttccga ctttgagcat tacttcaggg
     2401 ttgcatcctg gcccggaaat gaattcatta attatgtgga aaaagaaaag aaaatgacct
     2461 gggaacaggt ttataacaca ttgagttaca tcgatataaa aaacctggct ggctggatca
     2521 aagcgccaat gctgatggga gcaggtttgg ttgatgatgt ttgccctccg catattaact
     2581 ttgctgctta caaccaggtg aaatccggga aacactatat cgtttatccg ctttccggac
     2641 acgggcttcc cgaagacttc cacttccgga aaatggactg gatccggtcg catttcggat
     2701 tgaaatagtg ttcacggaat ttcatcttta accaaaaatt taaaacatgc attttggcaa
     2761 atcaggtcag ctgctgctga cgatcatcat gacaatgata ttttcgcatt cgatggcggc
     2821 atcggatgta agagcccgtg ataatttcaa tcagggttgg aggttttatc ctggcgacat
     2881 gccggaagcc aggaattata atttcgacga ttcacaatgg cggcagcttg atttgccgca
     2941 cgactggagc atcgaaggcg atttcagcga ccaaaacccc gccacacccg gaggaggggc
     3001 gttgcccgga ggtatcggct ggtaccgcaa gactttcaaa attcctgccg ccgacagtca
     3061 aaaactaatt tacatcgaat ttgacggcgt ataccgcaac agcaaagtat ggatcaacgg
     3121 gacctgcctc ggcaaacggc cttatggtta ttcttcgttt aggtatgagc tgacccccta
     3181 cctgaatttc ggcgacaaac ccaatgtgat cgcggttaag gtcgacaact cgcaacagcc
     3241 aaactcccgg tggtattccg gatcaggcat ttacaggaat gtctggcttg tgaaaaccgg
     3301 taaaatcgct gttgatcatt ggggaaccta tgtcaccacc ccgaaaatcg atcagaaagg
     3361 cgccgaagtt tttgtaaaaa taagagtcag aaactccccg ggaacaatgc ctgcgataag
     3421 catcaaatcc ggaatctata accagcagaa tgaactggtt gcagaggtta tcactccaaa
     3481 agtgaaactg gagaaaggca atactgaaat acagcaaaaa ctcgttgtaa aaaatcctgt
     3541 gctctggtcg gatgaaaagc ctgaaatgta ccgtttggtc acaacacttt acaccaaatc
     3601 gggaatactg gatgaatatg aaacgccttt cgggatcagg acgtttgaat ttgatgccgt
     3661 cagaggcttt atcctgaacg gcagaccagt gaagataaaa ggagtatgca accatcatga
     3721 cctgggatgc ctgggcgccg ccatcaataa acgcggcctt gagcgccagc ttgaactgct
     3781 gaaagaaatg ggctgcaacg gcatacgcac ttcgcataat ccgccggctc ccgaactgct
     3841 ggatctatgc gaccgcatgg gtttcattgt gatggacgaa gctttcgata tgtggaaaat
     3901 ggaaaagact ccctgtgact atagccgcga ttttgatgaa tggcaccaac gcgatatcca
     3961 ggatatggtg taccgcgaca gaaatcatcc ttcggttttc atatggagca tcggcaacga
     4021 aatccctgaa caatgggaca gcacaggttt ccggcttgcc agggaactgg ccggcctcgt
     4081 ccgcgaactc gataaaaccc gcccgatcac ctcggcctgc aacaatcccg acacttcaaa
     4141 ttacatcatt aaatcgggcg ttttggacct gatcggccag aattaccatc acggcacttt
     4201 cgccaatttc ccgaccatat accccggcaa gaaattcatt gccagcgaga ctacttccgc
     4261 tattgcaacc cgcggcagct atgatatgcc tgccgactct gtcagggtct ggcccagata
     4321 ttgggacaaa ccatttaccg acggcaatgc cgacctgagt tgttccgcct atgacaattg
     4381 ccgtgtaccc tggggatcga cacacgagga aacctggaaa atcattaaaa aatatgatta
     4441 tctgtcaggg ctcttcatct ggaccggttt cgattacatc ggcgaaccca ccccttatcc
     4501 gtggccggca cgcagttcat acttcggaat ccttgacctg tgcggtttcc cgaaagacgc
     4561 cttttatatg tataagagcg aatggacaag cactcctgtt ctacatttgc ttccgcattg
     4621 gaactggaaa gccgggcagg atgttgacgt ctgggcctat tcaaactgtg aggaagtgga
     4681 actgttcctg aacggccgtt cactgggaac gcagaaaaaa accggcgacc agatccattt
     4741 tgcctggaaa gtgccttttg aaccgggaac tctcagggcc atcggccgga caaacggaaa
     4801 gcaggtcctt accgatgaag tcaggactgc cgggctccct gccaggatca tccttacccc
     4861 cgaccgctcc tcgatccatg ccgacgggca ggatctttcc tacatcacgg tgcaggtcgc
     4921 ggatcaaaat aatatacccg ttccggatgc cggcaaccgg atatattttt cagtggaagg
     4981 tgatgctgcg atagcaggta cggataacgg cgaccccacc agtatggaat cgttcaaatc
     5041 gcatgaaaaa cgtgcttttt acggcagatg cctcctcgtg gtgaaagccg gcaataagga
     5101 aggaacggtt aaaataacag ccgtatccga tggccttcag tctgctaccg aaaatatcca
     5161 actcgtaaaa taacctgtat gaaaaaactc ctctttcttg cttttgtgat tgcagtccag
     5221 tctgcctact gccagaaatt cgacctggcc aaaaccccgc cgatgggatg gaatagctgg
     5281 aatacttttg attgtaacat cgatgaaaac ctgatccgcc agactgccga tgccatggtt
     5341 gccaacggca tgaaagacgc cgggtacgtt tatatcaacc tcgacgattg ctggcatggt
     5401 gaacgcgaca gtctgggttt catccacgaa gacccggtgc gttttccttc gggcatgaag
     5461 gcgcttgccg attacgtgca ttcaaaaggt cttaaaatag gcatttacag cgatgccggc
     5521 acacaaacct gcggcggcag accggggagc cggggccatg agtaccagga cgcggttacg
     5581 tatgccaact ggggcatcga ttacctcaaa tatgactggt gcagtaccgg cgaactgaac
     5641 gccaaagaag catatatcac gatgagggat gccattcatg ccgccggaag gccgatggtt
     5701 ttcagtatct gcgaatgggg ctcgaacaag ccatggacct gggcaaaaga ggtgggacat
     5761 ctgtggcgga cgaccgggga cattcacaat tgctgggatt gcgaaatggg tttcggcacc
     5821 tggtcaaagc tgggagtatt aagcatcctg gataaacaga atgatctgag aaaatatgcc
     5881 ggccccggcc attggaacga tcccgacatg ctcgaagtcg gcaacggcat gagctatacc
     5941 gaagaccggg cccatttttc gttatggtgc atgctggccg ctccgctcat atccggcaac
     6001 gacctccgga acatgccgaa ggaaaccctt gatatcctca ccaacaagga tgccatcgcc
     6061 ataaaccagg atgaactggg agtgcaatgc tataaaatgc tggacatggg caattacgaa
     6121 atctacgcca aaccactgag caacaacgaa ctggcgcttt gtttcctgaa ccgtggcgaa
     6181 acaccctgga atatcgattt caactggaaa aattatccga tatatgacgg catctccgga
     6241 aaaggctatg attttgtaaa gacaaagttt aaaatccatg actgctggca aaagaaggat
     6301 accggcacga ccgccgaacc attcaaaccg gtgcttcagc ctcatgatgt gatactgata
     6361 cggttgacgc cggtgaaatg atctcgttcc ggtattttta aatgttcatc aaacatggct
     6421 gctatgaaaa ggagaattca atgccgtcag aatcggtttt aaccacgaat tacacgaagc
     6481 cggtaccgac ggattacata gcgaaccacc tgttttatag gtaagcagcc acaaaaagca
     6541 cgaagcaaaa cgccgaagcc gccggttcat tactgtgaat tccgccggat gctttttgtg
     6601 acctgtcacc gaaaaattat aaccacgaat tacacgaagc ctgtaccgac ggattacaca
     6661 gcgaaccgcc tgttttttaa gggagcagcc acaaaaagca agaatcaaaa caccgaagtc
     6721 gccggttcat ttcagcgaat tccgccggat gctttttgtg acctgtcacc gaaaaatcat
     6781 gaccacgaat tacacgaagc ctgtaccgac ggattacaca gcgaatcacc tgttttatag
     6841 gtaagcagga acaaaaagca cgaatcaaaa cgccgaagcc gccggagcat ttccgtgaat
     6901 tccgccggat gctttttgtg acctgtcacc gaaaaattat aaccacgaat tacacgaagc
     6961 ctgtaccgac ggattacaca gcgaaccgcc tgttttttaa gggagcagcc acaaaaagca
     7021 agaatcaaaa caccgaagtc gccggttcat ttcagcgaat tccgccggat gctttttgtg
     7081 acctgtcacc gaaaaatcat gaccacgaat tacacgaagc ctgtaccgac ggattacaca
     7141 gcgaatcacc tgttttatag gtaagcagga acaaaaagca cgaatcaaaa cgccgaagcc
     7201 gccggagcat ttccgtgaat tccgccggat gctttttgtg acctgtcacc gaaaaatttt
     7261 aacaaccctc acgagacaag attttcttcg ggaattccgc ctgtttaatc aacaattcag
     7321 caacttaaat gttagttttg tcagattaac agtatatgtc tgaaattaaa aaagaatcat
     7381 tcccggttac cgggatggcc tgtgcagcct gcgccgtcag tgtgcagtca atgctcggat
     7441 cccggaaagg cgtggtatca gcccgggtaa attacggcaa caggaccgtc aggctggaat
     7501 acgacccttc gatagtagcg atcggagaac ttcgcaacct ggttcaaagc atcggttatg
     7561 acatgatcac cgaagaggaa aaccgctccg ggaaacttga aatagtcgag cataagagga
     7621 agctgatttt acggaacaga ctcattattg cagccttatt cagcgtcccc gtatttctcc
     7681 tctccatgtt ctttcacaac attctcccct atcagcatta cattctcttg attctatcgc
     7741 tgccggtcat cctgtattcc ggcgctgaat tttatcccgc tgctatccga caggccaagc
     7801 acggaatatt taccatggat tcgctggttt ccaccggaac aggagcggct ttcctgctga
     7861 gtattttcaa cactttgttt cctgaagtgc tgcaatcgca gggaatggag gcttatgtgt
     7921 attatgaatc agccgtcatc attatcacgt tcatccttac aggccgatat attgaggaaa
     7981 atgtaaaagc acgagcatcg tcggctatcc gaaaactgac aggcctgcaa cccaaaaaaa
     8041 taatgatccg gcgcgaaggc gaactgtttg aaatcccggc agtacatgta attcccggcg
     8101 atgtagtgac cgtccggtta ggcgaccgga ttccggttga tggaaccgtt gtaacaggtt
     8161 cggcttcggt ggacgaaagc gcgctcaccg gcgagcccat gccggtcgcc aagcaacctg
     8221 ccagccaggt ttatgcaggg acaatgaaca tacaggggct gatagaagta gaagcccgaa
     8281 aacccggtaa tgaaaccatc ctttctcaaa tcatcagcct cgtggatgaa gcgcaatcgt
     8341 cgaaaccgcc tatccagcaa tacgtagacc gaattgccgg ggtttttgtt cctgcggttt
     8401 ttatcattgc agccattact tttttatcct ggttgttcat cggccattcc atcagccatg
     8461 ctttcgtaac caccatcagc gtactcgtga ttgcctgtcc ctgtgcgctt gggctggcta
     8521 cgccgatggc gctgattgcc ggtatagggc gcggcgcttc ccggggaatg attatccgca
     8581 acgcacggtc tcttgaagat gcagcaggaa ttgacgttgt cgtcctggat aaaaccggca
     8641 ccatcaccag cggaaagcct tccgtatcca tccggtggct gacggaaacc ggtgacctgc
     8701 catttttact ttactgcgcc gaaaaacaat cggcccatcc actggcggcc gcaatatgca
     8761 attatcttga atcttcattc gaattatggg gccggcctcc gttggtgttt gaatcattcc
     8821 gggaaacagc ggggaaaggc attgcagtgg tctggaatgg cacgaaattc cttatcggaa
     8881 gtgaacagta cctgttatcg gaaaatgtaa aaattgagaa aggaaccgaa gactccggaa
     8941 aatcaaatcc agccatttat gtggctattg aaagccagcc ggcagcaact gtcgaaatta
     9001 ccgataaact caaaccttcg tcaaagaaag ccgtggaaag gctcagagcg ctcggcattc
     9061 ataccgtgct ggcttcaggc gattcagcga aacccgccca aaccattgcc tctgaagcag
     9121 gtgttgacaa agtatatcac agtattctcc ctgatagaaa aggagaaatc atcaaagagt
     9181 tgcaatcaga agggcataaa gtagccatga tcggcgatgg tattaatgat gcctatgccc
     9241 tggcacaagc tgacctgggt attgccatgg gcaacggcac cgacgtagcc atcgaaagcg
     9301 ccggcatcat cctgatgaaa ccggaactac cattagccgt gagcgcaatc cgtctttcaa
     9361 aatgggtggt ccggctgatt cgccagaacc tgttctgggc atttttttac aatgtgattg
     9421 ccatcccgtt ggcggcaggt ctgctttatc ctgtgaacgg attcctcctc agcccgatgg
     9481 ttgccggggc ggccatggcg atgagttcac tcactgtcgt caccaacagc ctcaggctga
     9541 aatatgttaa aattgaataa ggaaacggag aacggtgaac ggagaacggt gaacggagaa
     9601 ctttacctgc cggtgtatgg ttttgtgtga gaggtaggca ggggaggacg cagaacacga
     9661 atcacgagcc acgaatttca cgaatgcaat cgcgaatacc acgaattact agtcacgaaa
     9721 cacgaacaac gaatcacgaa acacgaattc cacgaatgcc atcgcgaatt ccacttaaaa
     9781 cacaaaacac taaacacaaa acgctgaaca ctgaaaccct gaaaccctga aacactgaaa
     9841 cactgaaact ttgcctacga acaacgaaac acgaaacgca aaacgcaaaa cgcagaactc
     9901 tggaacactg aaacactgaa actttgccca caaacaacga aacgcaaaac tcataacgca
     9961 aaactcaaaa cgcagaaccc tggaacactg aaacactgaa acactgaaac actgaaactt
    10021 tgcccacgaa caacgacacg caaaactcaa aacacaaaac gcagaactct ggaaccctgc
    10081 cccctcccac agcct
//
LOCUS       DAOPYV010000094        10075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_7819_length_10075_cov_14.310180, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000094 DAOPYV010000000
VERSION     DAOPYV010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10075
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7819_length_10075_cov_14.310180"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..2090)
                     /gene="metG"
                     /locus_tag="PKI35_10525"
     CDS             complement(33..2090)
                     /gene="metG"
                     /locus_tag="PKI35_10525"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNX44862.1"
                     /translation="MSDQSKYKRYTVTSALPYANGPIHIGHLAGVYIPADIYVRYLRS
                     KGEDVLFIGGSDEHGVPITIRARKEGLTPQQVVDKYHEMIRKSFEELGISFDIYSRTS
                     SPVHHETASQFFRKLYDQGNFIEKYSEQYYDEEAKHFLADRYITGTCPKCGFEKAYGD
                     QCESCGSSLSPTELINPKSALSGNTPVMKETKHWFLPLDQYEGWLREWILDSHQEDWK
                     TNVYGQCKSWLDGGLQPRAVTRDLDWGVKVPVEGAGDKVLYVWFDAPIGYISASREWS
                     QKTGKDWEPYWKSSDSKLVHFIGKDNIVFHCIIFPVMLKAEGSYILPDNVPANEFLNL
                     ESDKISTSRNWAVWLHEYLEELPGKQDVLRYVLAANAPETKDNDFTWKDFQLKNNSEL
                     LAIFGNFVNRTLVLTHKYFGGRTPACNTLDDYDKQVVEEIGKFPGKISESIDNYRFRE
                     ALGELMNLARLGNKYLTDTEPWKNFPANPDRVATNLNLALQICANLSCLCEPFLPFTA
                     AKLTGMLNIGALKWKEAGSVRILAPGHTLNAPAFLFEKIEDSVVEAQVQKLLDTKKAN
                     EAAQWQPSPQKPTITFDDFTKLDLRVGTIMAAEKVAKADKLLKLTVDTGLDKRTVVSG
                     IAAHYKPEEIIGRKVTILLNLAPRKLKGIESEGMILMAENREGKLSFVQPDEPFDSGS
                     EIR"
     gene            2594..6652
                     /locus_tag="PKI35_10530"
     CDS             2594..6652
                     /locus_tag="PKI35_10530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44863.1"
                     /translation="MKLLRNTLLLLPVLIFLTSNLSAQKQVSSRQPQTPFAGSAPEIS
                     YQPGLDYPDYPLLILPESYRGRQLPSSVDNSALPYMRPVFMQQGASCGQAASVGYDFC
                     YEIDRVRDLPADTSINQYPTHFTWNFTSTDDGMVGGGVSYFRTFEILRSYGCPNEADF
                     GPITMDDDYYWMSGYDKYLAAMHNRIRMVNSINVGTPEGLLTLKHWLNDHLEGSATGG
                     LANFYTGYGSVIPLPANTPEAGKGVITNWTSPAVHALTIVGYNDSIRYDINKDGLFTN
                     DIDINSDGIVDLKDWEIGGYKIVNSYGEDWANFGYSYALYRSLALDYGDGGIWNNAAH
                     VVTVEPDYAPLLTMKIKISHNKRGRIRVRAGISADTSSAYPETITGFSIFNFQGGDYY
                     MCGGNRAADKELEFGLDITPLIGAMKTGAPSRVFLLIDEKDPDNTAEGDVSQFSVISY
                     LNDTIEYNCGDAPSPIVNNSETMFSVVLPGNFPVPVITPEYLPAAVAGFPYSQHLTAS
                     SGYPPYKWQIQETYTTAISQADYVPGQGQLLSFPNTASGIAVCKLPFEFPFYGEKYDT
                     IRVQTDGYLFLENIEAPYPYVQDESLYLSQIKAISPYMNRFQQVTAAGTGVWFEADAS
                     HAVFTWKVDGESCDTTKSDNFKATLYPDGKIEFSYGALDIPLTQGAIAGISNGDGLNF
                     QFGDPDFVAPSALYSLIPEKLPGGLAISPDGVISSDTVQGDFSAKMTVAVTDSRNIHC
                     VKPYTLSTGPIVETSIRSGEDTVMEPGEEAVMSLLIHNLTSGNVHNLELRLQSLSPLV
                     AISDSTEECSDIPNGLDLNLADAFRFRVPEAGSQGRDLNFKLRLAWDNHSAEQLLTYS
                     QALQEYAFSGPLIIDGNNKRLDAGESCKLIFRVTYGGAPPEDTIMGNIYTDDPYVSVS
                     ETSLVPFLNPGGHSSYKLAEWNVKANPHTPPGRLVKFSFEGIPGKGDTMHETFNLGIG
                     KTPVAVIDLDGNKNSGRHIIASMEKLNVNPDQLYFIDSAICDYDNLFLCLGTKPIANY
                     HVLTDEEGAFLDYFLSQGNNLYMEGGATFGYDKQLPVHEKFSVKGKKLAWPYPADSIA
                     GDSSTFAEGIHFIYKGDNLMTDNLNPLDSAISIFTDENTHYHFTVINDACSYRTIASS
                     IEFGGFYPYGGSTREEIMKHYLDFFGYNSQSLAANFVTDHYTTCAGTSMEFRPNCAGS
                     PLTYHWTFEGGIPSGSEDQNAYVRWDEPGIYDVSLIVTDSVTSDTLVREGLITVLDCS
                     SIPEETVAFELNAYPNPVSQQLYLRANTNCPGFYELSLTDLVGRNMLSGEAFFPAGIS
                     VYPVDVSGMKKGVYILVLRNNHTRLTRKIVVN"
     gene            6691..7626
                     /locus_tag="PKI35_10535"
     CDS             6691..7626
                     /locus_tag="PKI35_10535"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNX44864.1"
                     /translation="MITPPFLKSGDTVAIVAPGRKVSPAEMEPAIRQLRSWGLKVITG
                     PHLFGQQDQYSGKDEERAADLQLMLDDETVRAIICARGGYGTVRIVDRLDFGLFSKNP
                     KWIVGYSDITVLHSHVNRQTGVETLHATMPINFPADGSENESTQSLHEALCGKVPEYE
                     TGPYPLNRTGKAKGILTGGNLSILYALNGTPSDMNTDGKILFIEDLDEYLYHIDRMMM
                     TLKRSGKLNNLAGMIVGGMSKMNDNTVPFGKTAEEIIAETVSGYGFPLCFGFPAGHLE
                     QNMSLIMGRNISMDVQPHKVTLAFDRETKENLCCA"
     gene            7708..8070
                     /locus_tag="PKI35_10540"
     CDS             7708..8070
                     /locus_tag="PKI35_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNX44865.1"
                     /translation="MAEHNDLGKLGEELATKYLIGKGYPILARNWRNGRDEIDIIARD
                     GEWLVIVEVKTRTSEYFGEPETAVTNAKQKSLARAAEAYIFAVDHKGETRFDVIGILF
                     DHGKVRLNHIRDAFLPRL"
     gene            complement(8097..9245)
                     /locus_tag="PKI35_10545"
     CDS             complement(8097..9245)
                     /locus_tag="PKI35_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNX44866.1"
                     /translation="MAKYSNDKDLFREGIITAGRYSYSRALILQSLLKGEGIECFLVT
                     HEPELQAFQSSVELKVRKGDIAKTMRLVAASAEGSGKRKEKALESIRTVRRILVPVDF
                     SNQSVNAGRFAATLANKFKAEVTLLHVYYNPAIDVTPYDDHYAYQVKLASFLHEIEVT
                     ARENMAQLQDNLKKWAVKKGFKTLRLVPAFLNGPATAEILAFAENYNPAVIIMGTRGL
                     SKEEEKGYGRTASSIINMAKFPVLALPEGPVKEITDVKRIMYATDLDDSDFSFINRLI
                     AMVAPFGCRLHCVHVSLGMKKPWEKVRLEQLHDYLTGQYAGQHITFSHLVGDSLMNSL
                     ETYVRDHKIDLITVVSHKRRFPTKLFTESVTRRIFSEIKKPLLVFHTK"
     gene            9435..>9942
                     /locus_tag="PKI35_10550"
     CDS             9435..>9942
                     /locus_tag="PKI35_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44867.1"
                     /translation="MRNNFRKGSDKPDRGAGRRKEYTGERPSRKKSSSDNPGEERESK
                     PFRKYPEKTGRSGRSSGYNAPGESGPGKGKRPYRSGSDKPAREKPFHRKDFDRSEEKP
                     FRKEREDRPYNRESGPKPYRRKEQDRPEEKPFRKEREERPYNRESGPKPYRRKEQDRP
                     EEKPFRKER"
     assembly_gap    9943..10042
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2490 a         2499 c         2481 g         2505 t
ORIGIN      
        1 cacgaattga aaaatcatcg ctttcgccga agttaccgta tctcgctgcc gctgtcgaaa
       61 ggttcgtcag gctggacaaa gctaagcttt ccttcgcggt tttcggccat caggatcatt
      121 ccctccgatt cgatgccttt cagtttgcgg ggagccaggt tgagaaggat cgtgactttt
      181 cgcccgatga tctcctccgg tttataatgc gcagcaatgc ctgaaacaac cgtgcgctta
      241 tcaagtcccg tatcaacggt gagttttagc agtttatcgg ccttggcgac tttttcggcc
      301 gccataatgg ttcccacccg caggtcgagt ttggtgaaat catcgaaggt gatggttggc
      361 ttttgcggcg atggctgcca ctgagccgct tcattggctt ttttggtgtc gagaagtttc
      421 tgcacctgcg cttccaccac cgaatcctct atcttttcga acaggaaagc cggagcgttc
      481 aaagtatgtc ccggagccag gatacggacg cttcctgctt ctttccattt caatgcgccg
      541 atattcagca ttccggtaag cttggcggct gtgaatggca ggaacggctc acacaggcag
      601 gagaggttgg cgcaaatctg cagggcgaga ttcaggttag tggctacccg gtcgggattg
      661 gcgggaaaat ttttccacgg ttcggtatcg gtcaggtatt tgttgccgag gcgggccagg
      721 ttcatcaatt cgcccagcgc ttcgcggaaa cggtaattat caatgctttc cgaaattttg
      781 ccgggaaatt tgccgatctc ctcaacgacc tgtttgtcgt agtcatccaa cgtattgcag
      841 gcaggcgtcc tgccgccgaa atatttatgc gtcaacacaa gggtgcggtt cacgaaattg
      901 ccgaaaatag ccagcaactc gctgttgttc ttcagttgga agtccttcca ggtgaaatcg
      961 ttgtccttgg tttcgggtgc attggcggcc agcacataac ggagcacatc ctgcttgccg
     1021 ggcaactctt ccaggtattc gtgcagccat acagcccagt tacgcgacgt ggaaatttta
     1081 tcgctttcga gattcaggaa ttcgttggcc ggaacattgt cgggcagaat gtaactgcct
     1141 tcagccttta gcattaccgg gaagatgatg caatggaata cgatgttgtc cttgccgatg
     1201 aaatggacca gcttgctgtc ggacgatttc cagtaaggct cccaatcttt tcccgttttc
     1261 tgcgaccatt cgcgcgatgc cgagatatag ccgatgggag catcaaacca cacatacaga
     1321 actttatccc cggcgccttc aacaggcact tttacgcccc agtcgaggtc gcgggttacg
     1381 gcgcgcggtt gcagcccgcc gtcgagccac gacttgcatt gcccgtaaac gttggttttc
     1441 cagtcttcct ggtgcgagtc caggatccac tcccgaagcc acccttcata ttgatccaac
     1501 ggcagaaacc aatgtttggt ttctttcatc acgggagtat tcccgctgag ggccgatttt
     1561 gggttgatga gttcggtagg gctgagcgat gaaccgcaac tttcgcactg gtcgccgtaa
     1621 gctttttcga atccgcattt gggacaggtt ccggtgatgt aacggtcggc aaggaagtgt
     1681 tttgcttcct cgtcgtaata ctgctcactg tatttctcga tgaaattacc ctggtcgtaa
     1741 agtttcctga aaaactgcga agccgtttca tgatgcaccg gcgaagaggt cctgctgtaa
     1801 atatcgaagg aaatccccag ttcctcgaag gatttccgga tcatttcgtg gtacttatca
     1861 actacctgct gcggtgttag gccttcctta cgggcgcgga tggtgatggg tacgccatgc
     1921 tcatcgcttc cgccaatgaa taaaacatct tcgcctttcg accgtaaata gcgcacgtat
     1981 atgtcggccg ggatgtaaac gccggcaagg tgcccgatgt gaatgggacc gttggcatac
     2041 ggcaatgcgg atgtaaccgt atatcgctta tatttggact gatcgctcat gagttcgtaa
     2101 tagatcgtga aaagaacggc aaagataagg attctgtaaa agaggatcat aaacgtaatt
     2161 tcaaagagcg tgaaatggag gttgggtgat tataactatc aactgtcgga cattgagttg
     2221 taactgaaat gaaggcctgg aaaggaaacc ggcatctccc tgagtccggc agccctgatt
     2281 tcattcaggc gccggatact gcggttcacg gctaaacaac cgggataaac gaagtcggta
     2341 caaacgagcg ttcaaattct gggggcctga atttcttaat ccattcaatc cggataattt
     2401 cgccggcaaa ccggcagccg gatacccaaa ggctggcagg gcgcacccgc ccttcagatt
     2461 taatgtatta tccggtaaat ctgttcagtc aacggttttt acaggaagat tttccggtat
     2521 ttcagccatt caaaatacta tgcaaacaca tttcaaaatt tctaactttg taaataacaa
     2581 tcatgcggtt attatgaaac ttttaagaaa tactctgttg ctcctccctg tactgatttt
     2641 cctgaccagc aatctttccg ctcagaaaca ggttagttcc cgtcagcctc agacgccctt
     2701 tgccggttct gccccggaaa tcagttacca gccgggattg gattatccgg attacccgtt
     2761 gctgatttta cctgaatcat accggggccg gcagcttcct tcatccgttg ataattctgc
     2821 cttgccttat atgcggcctg tattcatgca gcagggagcc tcctgcggac aggctgccag
     2881 cgtcggctat gatttctgct atgagattga cagggtacgg gaccttcctg ccgatacctc
     2941 cattaaccaa tatcccacac attttacctg gaatttcacc agtaccgacg atggaatggt
     3001 tggcggaggc gtcagctatt tccgcacctt tgaaatcctg aggtcttacg ggtgcccgaa
     3061 cgaagccgat ttcggcccta tcaccatgga tgacgactat tactggatga gcggctacga
     3121 taaatacctg gctgccatgc acaaccgcat caggatggtc aattcaatta acgtagggac
     3181 cccggaggga ctgctgacgc tcaagcactg gctgaatgat cacctcgaag ggtcggcaac
     3241 aggcggatta gccaattttt atactggtta cggatcggtt atccctttgc ctgccaacac
     3301 gcccgaagcc gggaaaggcg ttattaccaa ctggacgtca ccggccgtgc atgcgcttac
     3361 tatcgttggc tacaatgatt ccatacggta tgatatcaac aaagacgggt tgttcaccaa
     3421 tgatatcgat atcaattccg acggcattgt ggatttaaag gactgggaga ttggcggtta
     3481 taaaattgtt aacagttatg gagaggactg ggccaacttc ggctattctt atgcacttta
     3541 ccggtctctg gcactggatt acggagatgg cggaatatgg aacaatgcgg cgcatgtggt
     3601 taccgtggag ccggactatg ccccgctttt aaccatgaaa ataaagatca gccacaacaa
     3661 acgggggcgt atacgcgtga gggcaggcat cagcgccgat accagttctg catatcccga
     3721 aacaatcacc ggcttttcca tcttcaattt tcagggaggc gattattata tgtgcggggg
     3781 aaacagagcc gccgataaag aattggaatt cggcctggac attacgcccc tgatcggagc
     3841 aatgaaaacc ggcgcccctt caagagtttt cctgcttatt gacgaaaaag acccggataa
     3901 tacggcagag ggcgatgtca gccaattctc cgtcatcagt taccttaatg atacgataga
     3961 atataactgc ggtgatgccc cttcccccat cgtaaataac agtgagacca tgttttctgt
     4021 tgttttgccg ggcaattttc cggttcccgt aatcacgccg gaatacctgc ctgccgccgt
     4081 tgccggattt ccatacagcc agcatcttac tgcttcttcc ggatatcctc cctataagtg
     4141 gcaaatccag gaaacataca ccactgcgat cagccaggcg gattatgtac ccggtcaggg
     4201 acaacttctc agctttccga atactgcaag cggaattgcc gtttgtaaac tgccctttga
     4261 atttccattc tatggggaaa aatatgatac catccgggtt caaaccgatg gatacctttt
     4321 tcttgaaaac atcgaagcgc cctatcccta tgtccaggat gaatcactct acctttcaca
     4381 gatcaaggcc atttcgcctt acatgaacag gtttcagcag gtcacggctg caggaacggg
     4441 cgtctggttc gaagccgatg cctcgcacgc tgtttttacc tggaaagtgg acggggaaag
     4501 ttgcgatacc accaaatccg ataattttaa agcgaccctt tatcctgacg ggaaaattga
     4561 attctcctat ggggcgctgg atattccgtt gactcagggc gcgattgccg gcatctcgaa
     4621 cggggacggc ctgaacttcc agtttggcga ccctgatttt gtggccccat ctgccttgta
     4681 cagcctgatt cccgaaaaac tccccggcgg acttgccatc agccctgacg gtgtgatttc
     4741 gagcgataca gtgcagggcg atttctcggc gaaaatgacc gtggcggtga ccgacagcag
     4801 gaatatccac tgtgtaaaac cctataccct gtcaaccggc ccgattgtgg aaacttccat
     4861 ccgaagcggc gaagatacag tcatggaacc cggtgaagag gccgtcatgt cgcttctgat
     4921 ccataatctt acctccggaa acgtccataa cctggaactc aggctgcaat ccttatcacc
     4981 gctggtggcc atatcggatt caaccgaaga atgttcagat atcccgaacg gccttgatct
     5041 gaacctggct gatgctttca ggttccgggt ccccgaagca ggcagccagg gccgtgatct
     5101 gaatttcaaa ctcagactcg cctgggacaa tcactcggcc gagcagttgc tgacctattc
     5161 gcaggcctta caggaatacg ccttttccgg gcctttgatc attgacggga ataacaaacg
     5221 gctggatgca ggggagtcct gcaagcttat attccgggtt acttacggag gggctccccc
     5281 ggaagatacc atcatgggta atatttatac cgacgatccc tatgtttcgg tttcggaaac
     5341 gtcgttggtg ccgttcctga accccggagg ccacagcagt tataaacttg cggaatggaa
     5401 tgtcaaagcc aacccgcata cccccccggg ccggctcgtc aaattttcat ttgaaggtat
     5461 cccgggaaag ggagacacca tgcatgaaac tttcaacctg ggtataggta aaacgcctgt
     5521 cgcagttatt gacctggatg gaaataaaaa ttccgggagg catatcattg ccagcatgga
     5581 gaaactcaat gtaaaccccg accagcttta cttcatcgac agcgctatct gtgattatga
     5641 taatctgttt ctatgcctgg gaacaaaacc gattgctaat tatcatgtgc ttacggacga
     5701 agaaggcgcg tttctcgatt attttcttag ccaggggaac aatctgtata tggaaggcgg
     5761 ggccacattc gggtacgaca aacagcttcc tgttcatgaa aaattcagtg tgaaaggaaa
     5821 gaaactagcc tggccctatc ctgccgacag tattgccgga gattcctcaa ctttcgccga
     5881 aggcattcat tttatttata aaggcgataa cctgatgacg gataacctga acccgctgga
     5941 ttccgccatt tccatattta ccgatgaaaa cacgcactat cactttacag taattaatga
     6001 tgcctgttcc tatcgcacga tagcttcatc cattgagttt ggagggtttt acccctacgg
     6061 gggatcgaca agggaagaaa tcatgaaaca ttatcttgac ttcttcggct ataattctca
     6121 atccctggcg gcaaacttcg tgacggacca ctacactacc tgtgcaggaa cctctatgga
     6181 attcaggccg aattgcgccg gttcccctct cacatatcat tggactttcg agggaggaat
     6241 tccgtcggga tcagaagatc agaacgccta tgtaagatgg gatgaaccgg gcatttatga
     6301 tgtaagcctc atcgttaccg attcggttac cagcgatacg cttgtcaggg aaggcctcat
     6361 cacggtgctg gactgcagca gcatcccgga agaaaccgtc gcttttgaac ttaacgcata
     6421 tcctaatccc gtttctcaac agttatatct gagggctaat accaattgtc cgggatttta
     6481 cgaactttcc ctcaccgatc tggtcggcag gaatatgctc tccggggaag cattcttccc
     6541 agccggtatt tctgtgtacc cggttgatgt gtcgggaatg aagaaaggcg tttatatttt
     6601 ggttctccgg aataatcata cccggcttac aagaaagata gttgtaaatt agcctcgttt
     6661 ttatctgcat tagtttttaa cagccgactc atgatcactc ctcccttcct taaatccggc
     6721 gatacggttg ccatcgttgc tccggggcgg aaagtgtcgc ccgccgaaat ggaacccgct
     6781 atccggcagc tcaggagttg gggactgaag gtgataaccg gaccgcatct gttcgggcaa
     6841 caggaccaat actcgggtaa ggatgaggag agagctgccg atctgcagtt gatgcttgac
     6901 gatgaaacag tccgtgccat catttgtgca aggggcggct atggaaccgt caggatcgtc
     6961 gacaggcttg atttcggtct cttttcgaaa aatcccaaat ggattgttgg ttacagcgat
     7021 attacggtcc tccattctca cgtcaacagg caaacaggcg ttgaaacact ccatgccacc
     7081 atgcccatca atttccctgc cgacggatcg gaaaacgagt ccacgcaatc gcttcacgaa
     7141 gccctgtgcg gcaaggtccc tgaatatgaa acaggcccat atccgttaaa caggacagga
     7201 aaagcgaaag gcatactgac cggcggcaat ctttcaatac tctatgcctt gaatggcact
     7261 ccttcggaca tgaataccga cgggaaaatc ttgtttatcg aagaccttga cgaatatctg
     7321 taccacatcg accgcatgat gatgacgctg aaacgctcag ggaagctcaa taatctcgcc
     7381 ggaatgattg tcggaggaat gtcgaaaatg aacgataaca cggttccgtt cggcaaaacg
     7441 gcggaagaaa ttattgcaga aaccgtttcc ggatacggtt tcccgttgtg cttcgggttt
     7501 ccggcaggtc atctggagca aaatatgtcg ctgattatgg gccggaatat cagcatggat
     7561 gtgcaaccgc ataaggttac cctcgcattt gaccgtgaaa cgaaggaaaa tctttgctgc
     7621 gcttaaactc aaggcggaga aatttctccg ccgtttctgt tcctggtttg ttttttatct
     7681 cctggtttta aagtaagtcc ggttaatatg gcagaacaca atgatctggg aaaactgggt
     7741 gaggaactgg cgaccaaata cctgatcggt aaaggttacc cgattctggc ccgaaactgg
     7801 cgaaacgggc gcgacgaaat tgatatcatt gcccgcgacg gcgaatggct ggtgattgtg
     7861 gaggtaaaaa cacggacatc ggaatatttc ggggaacctg aaacagccgt aaccaacgct
     7921 aagcaaaaat cgctggcccg cgctgccgaa gcctacattt ttgctgttga tcataaaggt
     7981 gaaacccgtt tcgatgtgat cggcatttta tttgaccatg gcaaggtcag gctgaaccac
     8041 atccgcgacg cttttcttcc gcgtctttaa tggaaagatg gcgggtaatc ccggcatcat
     8101 ttcgtgtgaa acaccagcag cggcttttta atctcgctga aaatcctgcg ggttacactt
     8161 tcggtaaaca atttggtggg gaatctccgt ttgtgcgata caacggtgat caggtcaatt
     8221 ttatggtcac ggacatacgt ttcgaggctg ttcatcagac tgtcgccgac caggtggctg
     8281 aaggtgatgt gctggccggc atactgaccg gtgaggtaat cgtgcagttg ttccagcctt
     8341 accttttccc agggtttttt catgccaagg ctcacgtgga cgcaatgcaa cctgcatccg
     8401 aaaggcgcaa ccatggcaat aagccggttg ataaatgaaa aatcggaatc gtcgagatcg
     8461 gttgcataca tgatgcgttt cacatcggtt atttctttaa cagggccttc gggcaaggct
     8521 aataccggga actttgccat gttgatgata ctgctggcgg ttcggccata acctttttct
     8581 tcctccttcg atagcccgcg ggtacccatg atgatcactg caggattata attttcggca
     8641 aaggcaagaa tttccgccgt agccggtccg tttaagaagg caggaaccag gcgcaacgtt
     8701 ttaaatcctt ttttcaccgc ccatttcttc aggttgtctt gcagttgcgc catattttcc
     8761 cgggcagtca cttcaatttc gtgcaggaaa ctggccagtt ttacctgata ggcgtaatga
     8821 tcgtcataag gagtaacgtc gatggccgga ttatagtaaa catgcagcaa ggtcacctcc
     8881 gctttgaatt tatttgccag ggttgccgca aaacgtccgg cattcaccga ctggttggaa
     8941 aaatcaaccg ggaccagaat ccgccgaact gtgcggatgc tctccagggc tttttctttc
     9001 cgtttccccg acccttccgc cgatgctgca accaatcgca tggttttggc aatatctcct
     9061 tttcggacct ttaattcaac gctgctctga aacgcctgta attccggttc gtgcgttacc
     9121 aggaaacact cgattccttc acctttcagc agactttgca atatcaaagc cctggaatag
     9181 gaataccggc cggccgtgat gatcccttcc ctgaaaagat ccttgtcatt actatacttt
     9241 gccatgaccc gaatgaattt ggatatatat acaaatataa cgaatttttt gcacttttgc
     9301 atattttata tcatgtattt tcattcacca tgtatgaaat cccggtaatt ccgggaaatt
     9361 atttatcagg aaccctgtcg ataacagatt gatgcatcct cagatagttc gtacctttgc
     9421 ggaaatttat atttatgcgg aacaacttca gaaaaggcag cgataaaccg gacagggggg
     9481 ccggcagaag gaaagagtat accggggagc gtccttccag gaaaaagtct tcatcagata
     9541 atccgggcga agaacgtgaa tcgaagcctt tccggaaata tccggagaaa acaggacgtt
     9601 ccggcaggtc ttcaggatat aacgccccgg gagaaagcgg gcccggaaaa ggaaaaaggc
     9661 cgtacagaag cggatccgat aagcctgcaa gggagaaacc ttttcacagg aaagatttcg
     9721 accgttcgga agaaaagccc ttccgcaagg agagggagga ccggccttac aaccgggaat
     9781 ccggccccaa gccataccgc aggaaagagc aggaccgtcc ggaggaaaaa cccttccgca
     9841 aggagaggga agagcggcct tacaaccggg aatccggtcc caagccctac cgcaggaaag
     9901 agcaggaccg tccggaggaa aaacccttcc gcaaggagag ggnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnaggaccgg ccttacaacc gggaatccgg tccca
//
LOCUS       DAOPYV010000095        10020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_7872_length_10020_cov_12.872353, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000095 DAOPYV010000000
VERSION     DAOPYV010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10020
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7872_length_10020_cov_12.872353"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..1193
                     /gene="hflX"
                     /locus_tag="PKI35_10555"
     CDS             9..1193
                     /gene="hflX"
                     /locus_tag="PKI35_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNX44868.1"
                     /translation="MLTSDLKPEKAVLIGLITAKQDENSVHDYLDELEFLLETAGAIP
                     GERFIQKLPMPDPRTFVGAGKLTEIAEYVKAAEIDIAVFDDELTASQTRNIEMELGCR
                     VLDRPLLILDIFAKRARTNTARTQVELAQYQYLLPRLTRMWTHLEKQRGGIGMRGPGE
                     KEIETDRRIIRDKIALLKEKLKDIDKQKVTQRKSREELIRVALVGYTNAGKSTTMNLL
                     SKSDVFAENKLFATLDTTVRKVVIGNLPFLLTDTVGFIRKLPHSLVESFKSTLDEVRE
                     ADLLLHIVDISHAAFEEQVDVALQTLAEIGCSDKPSIMVFNKIDAYSFIEKDPFDLTP
                     PTRENLTLEELQKTWMAKANAPAIFISAKQKTNIEEFRKLLYEEVKRLHVLRYPYNNF
                     LY"
     gene            complement(1208..1330)
                     /locus_tag="PKI35_10560"
     CDS             complement(1208..1330)
                     /locus_tag="PKI35_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44869.1"
                     /translation="MPVVTFKGPLPAFIEKAGNEVPVKEISNPVPDRKRSGGKL"
     gene            complement(1371..2747)
                     /locus_tag="PKI35_10565"
     CDS             complement(1371..2747)
                     /locus_tag="PKI35_10565"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HNX44870.1"
                     /translation="MIGVFVYLVKSIIAGALFLGFYNICMRKESFHRFNRYYLLGSAI
                     LMAIMPLAGSFLSVRMGGAAGATSLPVINLPEVVISATAIPPDQEKVLLNWTLVGYLV
                     ISAAMLAGLVISIIRISRIYRYSRNSETLGENIFVIASQGSPFSFLNRVFIPDSYKNH
                     PGLNAILIHEQAHIRQHHMADLILLEILSGIFWFNPFYFFIKRAMCEVHEYLADREVV
                     RHGTETLSYQQLLYNEVSGNPEYMIAKNFNLLTKKRIIMLIKKSSRNAAIRIGVLLPL
                     IVAAAFFVAITQSSILRAQDIPEPLTSAIQTPPAPPAPPVPPVPPAIQEPQKAEKAKV
                     KSSDKHAKPAKTEQVFTTVEKLPGFPGGDEARVNYMIENVKYPGEARQKGIQGVVFIS
                     FIVEKDGSITDAKVLRGIGGGCDEEALRVIKAMPKWNPGTHSGEPVRVQFNMPVKFKL
                     DNRPKTQQ"
     gene            complement(2744..3148)
                     /locus_tag="PKI35_10570"
     CDS             complement(2744..3148)
                     /locus_tag="PKI35_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNX44871.1"
                     /translation="MEKNRIPAKELTRAEEEVMQVLWQLNKGFVNDILDQYPEPKPAY
                     NTVSTIVRILEKKGFVDHKTFGKTFEYFPLVSKEDYTNHFMGNVVRGYFDNSYKKVVS
                     FFTGNDKISLKEMEEIRSIIEQQIQKKKGGDS"
     gene            3341..4978
                     /locus_tag="PKI35_10575"
     CDS             3341..4978
                     /locus_tag="PKI35_10575"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNX44872.1"
                     /translation="MIIIKPARYLITVLLLVVSETLLSQVFISSNLPIIIISTNNVPI
                     PDEPKIAGTMKIIDNGPGQINHITDSGNIYTGNIGIEIRGHYSASLPQKPYAIETRDA
                     DQKELNVPIFGFPEEHDWVLLANYNDKVFMRNVLAFRLFNRMGHYASRTRFAEVMVNG
                     VYQGIYIFGEKIKRDDGRVDIAKLTPDENSGEPMTGGYIFKIDYWNSSDSWLSNYHPT
                     GHPNFDVHYVYEYPKPEEISVQQKAYIKAYVNTFETALYNPFVHDSSAGYFRYMNTAS
                     FIDYFIVNEVSRNNDGFKKSFFMFKDRDGKDKRINLGPVWDFDWAWKNIDECWIFANT
                     DGSGWAYQVNDCYPDVNSPAWHVRLLQDSLFTDELRCRYELFRSGMLNTDTLMAWIDS
                     TAAYLGEAQQRHYTRWPILGLNVGTPVVGYIPTTFQGEIDGFKEWIRLRLDWLDANMP
                     GHCYDITSSHLSGMKPDIQAFPNPAKDYLYIELPSPCPDASIVTKNMAGKIVNPPYTF
                     DGNLFTIEVGSLTPGIYVAGINQRQGEMKHIKFVVVR"
     gene            complement(5087..6931)
                     /locus_tag="PKI35_10580"
     CDS             complement(5087..6931)
                     /locus_tag="PKI35_10580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNX44873.1"
                     /translation="MTAKQIPLEDFFKKPEKSGYQLSPDGKHYSYMAPYMRRMNIFIQ
                     EVGKDSAVRLTSDTARDIAGYFWGNNNRILYLKDTGGDENFKLFGVNIDGSNLVALTD
                     FPGVRTEIIDDLPDIDEYVIVGLNKRNPQVFDPYRLNINTGELTMLAENPGNIQAWIT
                     DHDGKLRLASALDGVNSSLLYRETENEPFKTILTTSFKEHVYPQFFTFDNKHIYASSN
                     LKRDKSVIVEFDPKTGKEVKALYENPDYDVEGLGYSRARKVLTAAYYTSWKTEMHFFD
                     KESESLFNKIKEQLGDYEISIGSSDKAEDKYIVRTYSDKSLGAYYLYDKASDKLSKIA
                     DVSPWIDENNMADQTPVEYKSRDGLTIHGYLTLPKGYTMKNAKNLPVVVNPHGGPWAR
                     DSWGFNPEIQFLANRGYAVFQMNFRGSTGYGRKFWESSFKQWGRSMQNDITDGVNWLI
                     EEGIADKNRIAIYGGSYGGYATLAGITLTPDLYAAAVDYVGVSNLFTFMKTIPPYWKP
                     MLDMMYEMVGDPVKDSLQMRETSPVFLVDKIKTPLFVAQGANDPRVNKDESDQMVKAL
                     KDRGINVKYMVKNNEGHGFHNEENRFDFYRAMEVFLGTHLAKKAEAGK"
     gene            7248..9836
                     /locus_tag="PKI35_10585"
     CDS             7248..9836
                     /locus_tag="PKI35_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNX44874.1"
                     /translation="MRYYTMKVRRFIKIFIISLSAIIILGAAAGLTLQYLFGGKVKEL
                     FVREINKQLATEVKVDNIQLSLFQDFPYASVRFTGVQMKEATKNKSGNNLLKAGVISL
                     KFNLPDLLRSRFSVRFISVTDAVVNIHIFPDGSDNFHIFKPSAGKQSEQFELNIKRFS
                     FKEVKFSYLDDEARQQAKLDIHRLMLKGDFKNEVFRMNAGGKLLVDKFSSGDIVYIAR
                     REIGLNAGVDIDTRTGLYAVNSGEIIMEKLHLSLNGWIYDHSDRKVMDVTVSALQTPA
                     REVFLLIPEKYRKQADNYQFAGSGDFSVVMKGIYGNGRIPSLTMKISMNDGSIIQRKT
                     AVGLENVSFSGVYFSPQDGSTMQLNLDNLKATLSNGSVTGNISMQGFKSPVIKATLAG
                     IFNLADLKNFLDLDTITRLSGMMNIAGVFSGSVADINNPVTSDFRNSRFSGTCTLNGV
                     SLGLKGYNLPVTALTGSFAFNNNDLNVNTLTGRFGSSDFSVKGSVGNLLSRIFVNNEK
                     LIVKGSLLAEKVDGDELSTSSGGKGEYNFGIPGDIEISGMKMDIRNFHFRKFDAKNLS
                     ATISMHNSVFTASDILLQSMQGTVTGEATINASNPAHSYLTCEVDVNKVNINRLFAEF
                     GNFGNTDLVAENLDGKVTGNVLFSGMMYPDLTIDLPSIKAHAELMVENGRLVNYAPMM
                     GLSSFLRVEDLKDIRFETLTNQIDIANEIIYIPVMDINSSALDLTLMGTHTFDNELNY
                     HFSIALADLLASKFRRHNPGYNKQDEFGPVASDGRRTMVFVSMTGTVDDPVFKYDKKA
                     VREKITTEMQGQRTELKQALNREFQWISGDSTKRAKILKEKEIQKKQEDGKFVIEWDD
                     DDRKGE"
BASE COUNT         2738 a         2269 c         2269 g         2744 t
ORIGIN      
        1 tataaataat gctcacttct gatctgaaac ccgaaaaagc cgttctgata ggcctcatca
       61 ccgcgaagca ggatgaaaat agtgttcatg attatctcga tgaacttgaa tttctgctcg
      121 aaacagcggg cgccattccc ggtgaacgtt tcatacaaaa attacccatg cccgatcccc
      181 ggacctttgt aggagccggg aaactaacag aaatagcaga atacgtaaaa gcggcagaaa
      241 ttgatattgc cgtcttcgac gatgaactca ccgcatccca aaccagaaat attgagatgg
      301 aactgggatg ccgtgtgctc gaccggcccc tgctgatcct tgatattttt gccaaacgcg
      361 cccgtaccaa caccgcccgt acccaggttg aactcgccca gtaccagtac ctgcttcctc
      421 gcctcacccg catgtggacg cacctcgaga aacaacgcgg cggaatcggg atgagaggcc
      481 ccggtgaaaa ggaaattgaa accgaccgga ggattatccg cgacaagata gcgctgctta
      541 aagaaaaact gaaagatatc gacaaacaga aagtgaccca gcggaaaagc cgcgaagaat
      601 taatacgggt cgccctggtc ggctatacca atgccggcaa aagcaccacc atgaacctgc
      661 tgagtaaaag tgacgtgttt gccgaaaaca agttgttcgc cacccttgat acaaccgtca
      721 ggaaggtggt gatcggcaac ctgccattcc tgctcaccga tacggtgggt tttatcagga
      781 agctgccgca cagcctggtg gaatcgttca aatcgacgct ggacgaagtg cgggaagccg
      841 acctgttgct gcatatcgtg gatatttccc acgctgcatt tgaagaacag gtggatgtgg
      901 cgctgcagac acttgccgaa atcggttgct ccgacaagcc gtcgatcatg gttttcaata
      961 aaatagacgc ttattccttc attgaaaaag atccctttga cctgacgcct ccgaccaggg
     1021 aaaacctgac gctcgaagag ttgcagaaaa catggatggc aaaagccaat gcaccggcga
     1081 ttttcatttc cgcaaaacaa aaaaccaata tcgaagaatt caggaaattg ctgtatgagg
     1141 aggtgaaaag gctgcacgtg ttgcggtatc cttataataa cttcctgtac tgatcgaaac
     1201 caacctttta taattttcct ccgcttcttt ttctgtcggg aacagggttg ctgatttcct
     1261 tcacgggaac ctcattccct gctttttcaa taaatgccgg taatggccct ttaaaggtca
     1321 ctaccggcat gatctgtctg atacagtaat tgaataaagg atcaagcctg ttactgctga
     1381 gttttcggcc tgttatccag tttaaacttc accggcatgt tgaattgaac cctgaccggt
     1441 tctccggagt gtgtcccggg gttccattta ggcatcgctt taataaccct gagggcttct
     1501 tcatcacagc cgccgccaat accccgtaaa actttcgcat cagtgattga accgtccttt
     1561 tcgacgatga aagagataaa aactactccc tggatacctt tctgacgcgc ttcacccggg
     1621 tacttcacat tttcaatcat ataattcacc cttgcttcat cacctcccgg aaaacctgga
     1681 agcttttcta ctgttgtaaa tacttgttcc gtttttgccg gtttcgcatg tttgtctgaa
     1741 gattttacct tggctttttc agctttctgg ggttcctgta tcgcgggcgg tacaggcgga
     1801 accggaggcg caggcggtgc aggcggcgtc tggatcgctg acgtcaaagg ctccgggata
     1861 tcctgtgccc gaagtatgct gctttgcgta attgctacga aaaacgccgc ggcaacaatc
     1921 agtggtaaca gtacgccgat ccgaatggcc gcatttctgg aagatttttt gattaacatg
     1981 atgattcgtt tttttgttaa cagattaaag tttttggcaa tcatatattc agggtttccg
     2041 gaaacctcgt tgtaaagaag ttgctgatag gatagtgttt cagttccgtg tctgactact
     2101 tcccggtcgg ccaggtattc atgcacttcg cacattgccc tcttgatgaa aaagtagaag
     2161 ggattgaacc agaatattcc cgatagtatt tccagtaaaa tgaggtcggc catgtgatgc
     2221 tgcctgatgt gtgcttgctc atgtatgagg atggcattta aaccggggtg gtttttgtag
     2281 gaatcaggga tgaataccct gttcagaaat gaaaaaggac tgccctgaga agcaatcacg
     2341 aagatattct cccctaatgt ctcggaattc cgcgaataac gataaatccg gctgatcctg
     2401 ataatgctga tgaccagccc ggccagcatg gctgccgaaa taaccaggta accaacaagc
     2461 gtccagttta acaagacttt ttcctggtcg ggaggaatgg ccgttgcaga gataacaacc
     2521 tcgggcagat tgatgacagg aagggaagtc gcgcctgctg cgccgcccat ccttacggaa
     2581 aggaaactgc ctgccaacgg catgatagcc atcagaatcg cggaacctag aagataatac
     2641 cggttgaacc ggtgaaagga ttccttccgc atgcatatat tgtaaaatcc caggaacagc
     2701 gctcccgcaa taatgctctt gaccagatag acaaagaccc cgatcatgaa tcgcctcctt
     2761 tctttttctg aatctgctgt tcgataatac tgcggatttc ttccatctcc ttcagggata
     2821 tcttatcatt tccggtaaag aatgaaacca cctttttata ggaattatca aaataccccc
     2881 tgaccacatt ccccataaag tggtttgtgt agtcctcttt gcttacaagc ggaaaatatt
     2941 caaaggtttt cccaaatgtt ttgtggtcta caaagccttt cttttcgagg atacgaacga
     3001 tcgtagaaac cgtgttgtag gccggctttg gctcaggata ttgatcgagg atgtcattca
     3061 caaatccttt gttcagttgc cagagcacct gcattacctc ttcttctgcc cgggtaagtt
     3121 ctttggccgg tatcctgttc ttttccattg tacttatttt gaggatgtaa agatacaact
     3181 aaattattag ttatgcaact aaataattag tttagacata aatattttat attatcatga
     3241 ttatatgtaa tttacgaaaa agcatctttt aatttttctg atgatcagga tttgttgatt
     3301 taataaattt gtcggcttaa gaacaaattg ttttacttgc atgattataa tcaaaccggc
     3361 caggtattta attaccgtac tattgcttgt ggtttccgaa accctgcttt cacaggtatt
     3421 tatctccagt aaccttccca tcatcattat cagtaccaat aatgtaccga ttccggatga
     3481 gcccaagata gcaggaacga tgaaaatcat cgacaatggt cccgggcaga taaatcatat
     3541 tacagattcg ggaaacatat ataccggaaa tatcggcatc gagattcggg gccattattc
     3601 cgcttcgctt cctcagaaac cttatgccat cgaaacccgg gatgcagatc aaaaggaact
     3661 gaatgtcccg atttttggtt tccctgaaga gcacgactgg gtcctgctgg ccaattacaa
     3721 cgacaaagtc ttcatgcgaa acgtactggc attcaggttg ttcaaccgga tgggtcatta
     3781 tgcttcccgc acgcgttttg cagaggtgat ggtaaacggg gtttatcagg gtatttacat
     3841 tttcggtgaa aaaattaaac gggatgatgg ccgggttgat atagccaagt tgacgcctga
     3901 tgaaaattca ggcgaaccga tgacgggagg gtatattttc aaaattgatt actggaattc
     3961 atccgatagc tggctttcaa actatcatcc caccggccac ccgaatttcg acgttcacta
     4021 tgtttacgaa tacccgaaac cagaagaaat cagtgtacaa caaaaagctt atatcaaggc
     4081 ttatgtcaat accttcgaaa cagcccttta taatcctttc gtccatgatt catcggccgg
     4141 gtatttccgt tatatgaaca cagcttcctt catcgattat ttcatagtga acgaagtctc
     4201 gcgaaataac gacggcttca agaaaagttt cttcatgttt aaagaccggg atggaaaaga
     4261 taaacggatc aacctggggc ctgtctggga ctttgactgg gcctggaaaa acattgacga
     4321 atgctggatt tttgccaaca ccgacgggag cggatgggca taccaggtaa atgattgtta
     4381 tccggatgtc aactcgccgg cctggcatgt caggttgtta caggactccc tttttaccga
     4441 cgaattgcgg tgccggtatg aacttttcag atctggcatg ctgaatacag atacactcat
     4501 ggcatggatt gactctacag ccgcttattt aggggaagcc cagcaacgtc attatacccg
     4561 ctggccgatc cttgggctaa atgtcgggac tcctgtagtc ggttatatac caaccacatt
     4621 ccagggcgaa attgacggtt tcaaggagtg gatccggctg cgccttgact ggcttgatgc
     4681 caacatgccc ggacattgtt acgatattac ctcaagccac ctctccggta tgaaaccgga
     4741 tatacaggct ttccccaacc cggcgaagga ttacctttat attgaacttc catcaccatg
     4801 ccccgatgcc tcaatcgtga ctaaaaatat ggccggaaaa attgtaaatc ctccctatac
     4861 gttcgacggg aatcttttca cgatagaggt cggttccctt acgccgggca tctatgttgc
     4921 cggtattaat caaaggcagg gggagatgaa acacattaaa tttgttgttg taagatagtc
     4981 ctgaaacctg caacatttcc cggttaaggc aacgacaggt gagccaggaa aactccaaag
     5041 cccggcctgt aggccgggct ttggatataa ttaaccaaaa caactattat tttcccgctt
     5101 ctgctttttt ggcaagatgc gttcccagga atacttccat agcacgatag aaatcaaaac
     5161 ggttttcctc gttatggaaa ccatgacctt cattattctt gaccatatat ttcacgttaa
     5221 ttcctctgtc tttcaatgct tttaccatct ggtcgctctc atctttgttt accctcgggt
     5281 cgttggctcc ctgagcgaca aacaaaggcg ttttgatttt gtcaaccaga aaaacaggtg
     5341 atgtttcgcg catctggaga ctatctttta cgggatcgcc gaccatttcg tacatcatgt
     5401 cgagcatcgg tttccagtaa ggaggaatag ttttcatgaa agtgaacagg tttgacacgc
     5461 cgacataatc aacagcagca gcatacaggt caggggtcag ggtaataccg gcgagagtgg
     5521 catagccgcc ataactgccg ccataaatag ctatacggtt tttatcggca ataccttctt
     5581 caataagcca gtttacgccg tccgtgatgt cattctgcat acttcttccc cattgtttaa
     5641 aggatgattc ccagaatttc cttccataac cggtcgatcc gcggaagttc atctggaata
     5701 cggcatagcc acggtttgcc aggaactgga tttcggggtt aaatccccaa ctgtcacggg
     5761 cccaggggcc tccgtgcgga ttcacaacca ccgggaggtt tttcgcattt ttcatggtat
     5821 aacctttcgg aagggtgagg taaccgtgta ttgtaaggcc gtcgcggctt ttgtattcaa
     5881 ccggggtttg atcggccatg ttgttttcat ctatccaggg gcttacatcg gcaatcttgc
     5941 tcaatttatc cgatgctttg tcataaaggt agtaagcgcc cagggactta tcgctgtatg
     6001 tccggacaat atatttatct tctgctttat cggagcttcc gatggatatt tcataatcgc
     6061 caagctgttc ttttatcttg ttaaacagag attcactctc tttgtcaaag aagtgcattt
     6121 ccgttttcca ggatgtatag taagctgcgg tcaatacttt cctggcacgc gaatagccca
     6181 ggccttcaac atcataatcc gggttttcgt aaagcgcttt aacctcttta cccgtttttg
     6241 gatcaaattc cacgattacc gatttatccc ttttcaggtt cgaagatgca tatatatgct
     6301 tgttgtcgaa agtaaaaaac tgcggataga cgtgttcctt gaagctcgtg gtaagaatgg
     6361 ttttaaacgg ctcgttttcg gtttctcggt atagcaacga actgttcacg ccatccaaag
     6421 cagaagccag ccgtagtttg ccgtcatgat cggtaatcca tgcctgaata ttgccggggt
     6481 tttcagccag cattgtcagt tcaccggtgt tgatattcag ccggtaaggg tcaaaaacct
     6541 gcggatttct tttgttcagg cccacaatca catactcgtc aatgtcgggg agatcatcaa
     6601 ttatctcagt tcttacaccc gggaaatcag tgagtgcgac caggttcgaa ccgtcgatat
     6661 tcacaccgaa taatttgaaa ttttcatcgc cgccggtgtc tttcaggtag agaatacggt
     6721 tattgtttcc ccagaagtaa ccggcaatat cgcgggctgt atcgctggtg agcctgacag
     6781 ccgagtcttt gcctacttcc tggatgaaaa tattcatgcg ccgcatatag ggagccatgt
     6841 atgaatagtg cttgccgtcg ggcgaaagct gatagcccga tttttcgggc tttttaaaga
     6901 aatcctcaag aggaatctgt ttggcagtca tttttccttc cttttgttta caacctaaag
     6961 cgcccaagat aataaaagcc atcatcagga ggattgccgg cattacattt tttttcataa
     7021 acagaagttt taatggttgg tggttacgat gaaactgatt gtcagacttt caaaattttt
     7081 cccgaattta tgacaaagca tggaatgcgg tatggctaag attggccaaa tctttcaatt
     7141 tgcagaaaat atctgtaaaa acctgtttat tgtcgatgaa atgacagagg ctgaattcat
     7201 gaattcatgg ttcaataata aacaccattg caacacatcc agtaactttg cgttactata
     7261 ccatgaaagt gcgacggttt atcaaaatct tcataatcag cctttctgcc atcataattc
     7321 ttggtgcagc ggcagggctg acgcttcaat accttttcgg aggaaaggtt aaggaactat
     7381 ttgtccgcga gatcaacaag cagttggcga ccgaggtaaa agtggataac atacaactaa
     7441 gcctgttcca agacttccca tacgcctccg taaggtttac cggtgtgcag atgaaagaag
     7501 caacgaaaaa taaatccggc aacaacctgt tgaaagcagg cgtaatttcc ctgaaattca
     7561 acttgcccga cctgttgcgg agccggtttt cggtgcgttt catatcggtt acggatgccg
     7621 tggttaatat tcatatattt cctgatggct ccgataattt ccacattttc aaaccgtcgg
     7681 ccggaaaaca aagtgaacaa tttgaactga atatcaaacg cttttctttc aaggaagtca
     7741 agttcagcta tctggatgat gaggcccgtc aacaggcgaa acttgacatt catcgcctta
     7801 tgctgaaagg tgattttaaa aacgaagtat tcagaatgaa tgccggaggc aaactattgg
     7861 tcgataagtt ctcttcaggc gacattgtgt atatcgcccg tcgcgagata gggctgaatg
     7921 cgggagtgga tattgataca aggacaggat tatacgcagt taatagtgga gaaattatca
     7981 tggagaagct ccatctctcg ttgaacgggt ggatttatga ccacagcgac aggaaagtga
     8041 tggatgtcac tgtatcggcc ttgcagacgc ctgccaggga agtgtttttg ctgattcccg
     8101 aaaaataccg gaagcaggca gataattatc agtttgcagg gtcaggggat ttttcagtgg
     8161 tcatgaaagg aatttacggg aacggaagga taccgtcgtt aaccatgaaa atctccatga
     8221 atgatggtag tataattcag cggaagacag cggttggcct cgaaaacgtt tcgttttcag
     8281 gcgtttattt ttctccccag gatggcagta ccatgcagct caaccttgat aacctcaaag
     8341 ccacgctaag caatggttca gttacaggga acatttcgat gcagggtttt aagtcgcccg
     8401 ttatcaaagc aactctcgcc ggaatattta accttgccga tctgaaaaat tttcttgatt
     8461 tggataccat cacccggctc tccggaatga tgaacatcgc cggcgttttt tcgggatcgg
     8521 ttgccgatat caacaatccg gtaacctccg atttccggaa cagcaggttt tcaggcacct
     8581 gtaccctgaa tggggtttcg ctgggcttaa aaggttataa cctgccggtt acagccctta
     8641 ccggcagttt tgcctttaac aacaacgatc tgaatgttaa cacattgaca ggccggtttg
     8701 gcagcagcga cttctcggta aagggatctg tcggaaatct cttatcaagg attttcgtaa
     8761 ataatgaaaa gctcattgta aaaggaagct tgctggccga aaaagtcgac ggggatgaac
     8821 tgagtacaag cagcggcggt aaaggcgaat ataatttcgg aatacccggc gatattgaga
     8881 tcagcggaat gaaaatggat atacgcaact tccatttcag aaagtttgat gcaaaaaatt
     8941 tgtcggcaac catttcgatg cacaacagtg tattcaccgc atccgatatc ctgctgcagt
     9001 cgatgcaggg cacggttacc ggcgaagcca ccatcaatgc ttccaaccct gctcatagct
     9061 atctcacatg tgaagtcgat gtaaataagg tgaatatcaa ccggttgttt gccgaattcg
     9121 gaaatttcgg aaatactgac ctggttgctg aaaaccttga cggcaaggtg accgggaatg
     9181 tattattttc gggcatgatg tatcccgacc tcacgattga tctgccgtcg atcaaggctc
     9241 atgcagaatt gatggttgaa aacggacgcc ttgttaatta cgccccgatg atgggcctgt
     9301 catcctttct gcgtgttgag gatttgaaag acatccgttt cgaaacgctt accaatcaga
     9361 ttgatattgc caatgaaata atttatattc ctgtaatgga tataaattcc agcgcattgg
     9421 accttaccct gatgggcaca catactttcg ataatgaatt gaactaccac ttcagcattg
     9481 ccctggccga tctgctggcg tcaaaattca ggagacataa tcccggatat aacaaacagg
     9541 atgagtttgg tccggttgct tccgacggcc ggcggaccat ggttttcgtt tcgatgacag
     9601 gaactgttga cgatcctgta ttcaaatacg ataaaaaagc ggtaagggaa aaaatcacga
     9661 cagagatgca aggccagcga acagaactta aacaggccct gaacagggag tttcaatgga
     9721 tttcgggcga ttcaaccaaa cgggctaaaa tactcaaaga aaaagaaata caaaagaaac
     9781 aggaagatgg aaaatttgtg attgaatggg atgatgatga caggaaagga gaatagattg
     9841 gtccctcagt tcaatgaata ttatgaaaac tcctgactct gaagaatgcc ggcttgatga
     9901 gatgacatta ttccagcctg gtattttcac ttactctgtc tctgtttcac ccggggttta
     9961 ttccggattc tatccttcct gtcccaaatt gaacaagaaa attattcttt aatatttatt
//
LOCUS       DAOPYV010000096         9990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_7915_length_9990_cov_12.903775, whole genome shotgun sequence.
ACCESSION   DAOPYV010000096 DAOPYV010000000
VERSION     DAOPYV010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7915_length_9990_cov_12.903775"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKI35_10590"
     CDS             <1..200
                     /locus_tag="PKI35_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44875.1"
                     /translation="PLRGSGGLVIDIEPLRGSGGLVIDIEPLRGSGGLVIDIEPLRGS
                     GGLVIDIEPLRGSGGLIFSVD"
     gene            complement(242..1675)
                     /gene="amrB"
                     /locus_tag="PKI35_10595"
     CDS             complement(242..1675)
                     /gene="amrB"
                     /locus_tag="PKI35_10595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNX44876.1"
                     /translation="MNAPDTLINRKPAVAGQFYPGSAAELQQTMKTLFAKAPAGHNSQ
                     EIKAIIVPHAGYVFSGEVTAAAFNQLDPSKEYKTVFIIGCSHRNSFAGASVYSIGNYI
                     TPFGEAPVDLEIARKLSIEHKALSFDPGYQQSEHSVEVQVPFIQYYLSKGYKIVPVLL
                     GTQDPSICKKIAEALKPYFTRDNLFVISTDFSHYPAYNDAVRVDMSIADAICTNRPDK
                     LIDAISNCMKMQVANLATGCCSWPSVLTLMYITEGEQDIVYNKVLYRNSGDSPYGEKD
                     RVVGYFAITASQPEKETTFKLSEKEKEQLLGIARNTINVYLGEGKIPEAFSGKIPEAL
                     LQKAGAFVTLRKHGELRGCIGHFDADQPIYSIVQQMAVAASTQDYRFSQVQPEEMKEI
                     EIEISVLTPMKKISSVSEIKLGRDGIYIRKGNRAGTFLPQVAKETGWGLEDFLGHCAR
                     DKAGIGWDGWKDRDTEIFTYQAYIFSE"
     gene            1814..2359
                     /locus_tag="PKI35_10600"
     CDS             1814..2359
                     /locus_tag="PKI35_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNX44877.1"
                     /translation="MAFLDLITARQSVRKYSQRPVEPEKLERCLEAARLAPSASNTQP
                     WHYIVVQDEPLRTKVAEATFSGPVKFNRFTLQAPVLVVILLEQPKLRNRLAMILKNKE
                     WKLIDIGITATHFCLQAAEEGLGTCMMGWFNEKQLKKVLSLPISKTIGLVISLGYAEE
                     DYPLRTKIRKPMEAIVSYNGF"
     gene            complement(2448..3233)
                     /locus_tag="PKI35_10605"
     CDS             complement(2448..3233)
                     /locus_tag="PKI35_10605"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNX44878.1"
                     /translation="MRRFQLILISVLLSTIGFGQYNEATRNYDLGNEYFQQKEYEKAI
                     EFYSISLSLYRAADAYYNRALSFIQLHDTCNYCKDMKNAAGLNDHEAFTLYKKICCFA
                     TVNPSLPDSIKIQVPGATHFEILHDLFGKDSTTYVISEVDGSIEAIEISDVSDYHVFT
                     VVEESPSFPGGADARAKFCSENLHYPPDAKKNNIQGTVFVNFIVERDGSISNVKVLRG
                     IGGGCDEESIRLIKSMPRWIPGKQRGKPVRVRFNMPVRFTLAG"
     gene            3403..5448
                     /locus_tag="PKI35_10610"
     CDS             3403..5448
                     /locus_tag="PKI35_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44879.1"
                     /translation="MKKLILLPALLLLIIAGCKQPSGKAGAPVTFINQSTMDKVIKQL
                     TDSCGEQAKFRIERGVKQAASLWQKEDGTAEEFATFCTENFVADSVKLQILFEKLQNA
                     FEVLYGNFNKMGMDLRVPVDLDGPELTPVDEIFASFSPSAHLTDDLFQNKIAFITALN
                     FPFYSLAEKTEQGVAWNRTQWAYARMGDVFTSRVPAELNQKVTEALSKSDMYISEYNI
                     MMGNLVGKTGKTCFPADMKLISHWGLRDELKSHYGKPDGLAKQQMIYSVMKRIIDQSI
                     PQDVINSKEYQWNPIDNKVLKGSKEVTFKSEPDTRYEILLNNFKAMKAVDPYYPAYPT
                     YSQRAFELGMEIPEADVEKLFVSLVTSPEIKQVGDLISKRLGRKLEPFDIWYDGFKAR
                     SSINEDELTAKTLRLYPDAKAFEKDMANILRKLGWSAERAGYIASRVQVDPARGSGHA
                     AGAYMKGDKARLRTRLTAQGMNYKGYNIATHEFGHNVEQTIDLYDIDNYMMQGVPNTA
                     FTEALAFMFQKRDLMLLGMKDNDPNKEHLAALDAIWSSYEIMGVSLVDMQVWRWMYDN
                     PNATPAQLKESVIRIAKEVWNKYYAPVFGSKDEPILAIYSHMIDNPLYLSNYPVGHLI
                     DFQVEGFMKGKNFASEVDRMYTQGRLVPQVWMKGAVGNTISVEPMLKAVDEALKVIK"
     gene            complement(5532..5891)
                     /locus_tag="PKI35_10615"
     CDS             complement(5532..5891)
                     /locus_tag="PKI35_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HNX44880.1"
                     /translation="MKTEFYPSDDTHIAEITSDEILIRTTQDALDLMVNCIYQGADRI
                     ILHQKNFIPAFFDLKTGIAGDILQKFSNYHTHLAIVGDFTEVKCKSLRDFIRESNRQG
                     RIVFTGTLEEAREKMAK"
     gene            complement(5932..6321)
                     /locus_tag="PKI35_10620"
     CDS             complement(5932..6321)
                     /locus_tag="PKI35_10620"
                     /inference="COORDINATES: protein motif:HMM:NF025903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HNX44881.1"
                     /translation="MPLVRVEIYKGKDKAYKQAILDGIHNALVSAFRIPESDRNQRIY
                     ELDEDDFERNTDKTDCFTIIEITAFEGRSTEAKRKLYRVISDNLKRDPGISETDIIVY
                     LNEPHLVNWGIKGHPASDIDLGFEIQV"
     gene            complement(6382..6855)
                     /locus_tag="PKI35_10625"
     CDS             complement(6382..6855)
                     /locus_tag="PKI35_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNX44882.1"
                     /translation="MDAADWPDVAEIYRQGIETGHATFEQKVPSWEEWNSRHLEACRF
                     VALSNGQIAGWAALSPVSERCVYAGVAEVSVYVGEKFRGQKIGSRLLEKLIGESGQNG
                     IWTLQAGIFPENDSSIRMHEALGFRRVGYREKIGKMNGRWRDTLLLERRSKTVGI"
     gene            complement(6994..>7419)
                     /locus_tag="PKI35_10630"
     CDS             complement(6994..>7419)
                     /locus_tag="PKI35_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNX44883.1"
                     /translation="EITREIEVAKDKALTKNDIVLNEKPKFYERLYYRLPGAFRRRIW
                     RILLRYPKIAYKKMGNVVVTSPGMMGTIKGWFIHKTVHPLSFGIGSVLKKPVVIGDEV
                     KVREILNMTILIDHDVIDGAPMVRFLNDLTRFIESGSEL"
     assembly_gap    7420..7519
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7520..7938)
                     /locus_tag="PKI35_10635"
     CDS             complement(<7520..7938)
                     /locus_tag="PKI35_10635"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNX44884.1"
                     /translation="MKQEVSLKLTLFLISLFLIPAISFSQGKYLLVLDVQEQFIRDKP
                     YEDAAGFMITNINSLIAGFEKDRILYVKSTGKSLVINSKGFSTDTLPVPDFDPRLSIV
                     NDNLFVKVSTGDAFTSAELMDFLRSRNAREIVITGLLA"
     gene            complement(8020..8559)
                     /locus_tag="PKI35_10640"
     CDS             complement(8020..8559)
                     /locus_tag="PKI35_10640"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX44885.1"
                     /translation="MLNRSEDMHDNMIETKRLYIKPLTAEELRKHVEEPEAFARELGL
                     APSQSLMEEEAKEAVLNDLLPNIINPSNNPLFYTMWLVIGKAEKAIIGGICFHGAPDE
                     QGEVEIGYGTDEGYRNRGYMAETIAGMMEWLREKKTVRKVRAETAVDNIPSLKVLGKN
                     GFRVVRQNENTVILLVELE"
     gene            complement(8572..9111)
                     /locus_tag="PKI35_10645"
     CDS             complement(8572..9111)
                     /locus_tag="PKI35_10645"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNX44886.1"
                     /translation="MNRTLRFIAGYLIGFTLFIVLVPYGFYRLSQCDAVFQGRLLISS
                     DLLRYVLSAVFFIAGAIFAAWSNLFLFTLGKGGPTDAFGVSISPQTQKLVTTGPYRYS
                     RNPMVFGAFSLYASLVIFLNSITGLIVLFCFLLLAILFLKKSEEKRLIRDFGEEYLEY
                     RKKVPMIFPVKLRNRKKVN"
     gene            complement(9189..9542)
                     /locus_tag="PKI35_10650"
     CDS             complement(9189..9542)
                     /locus_tag="PKI35_10650"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNX44887.1"
                     /translation="MFVGSQQRITINNTHHYVDMVFYNKILRSYILIELKSTKLKIKD
                     AGQLNTYLNYFKTEINDKDDNLPIGIILCAEKDEIAAEYILGGLENNVFASKYTYVLP
                     DKEELVRELENAIKE"
     gene            complement(9560..>9990)
                     /locus_tag="PKI35_10655"
     CDS             complement(9560..>9990)
                     /locus_tag="PKI35_10655"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HNX44888.1"
                     /translation="TTEFGKGFSRSNLFNMRKFYMEYPDVQTLSGQLSWSHICELLII
                     EDVQKRGFYEKESINSSWSVRELKRQIDSSLYERLLLSKGKTNKEQIIALSKNGMQIT
                     KPEDIVKSPYVFEFLGIPEYKPILEKELEQKLVRHLEEFF"
BASE COUNT         2574 a         2475 c         2142 g         2699 t
ORIGIN      
        1 agcccctccg gggctccggc ggtcttgtta ttgatattga gcccctccgg ggctctggcg
       61 gtcttgttat tgatattgag cccctccggg gctccggcgg tcttgttatt gatattgagc
      121 ccctccgggg ctccggcggt cttgttattg atattgagcc cctccggggc tctggcggtc
      181 ttattttttc agtcgattga tattgagccc ctccggggct ctgaccatct tatagttgct
      241 atcactccga aaatatatac gcctggtaag taaatatctc cgtatccctg tctttccacc
      301 cgtcccagcc gataccggct ttgtcgcggg cacaatgccc cagaaagtcc tctaaccccc
      361 atcccgtttc cttcgctacc tgcggcagga aggtccctgc gcggttacct ttccgaatgt
      421 atatcccgtc acgtcccagc ttaatttcgc tcaccgagct gatctttttc attggcgtaa
      481 gaaccgatat ttctatctca atttctttca tctcttcagg ctgcacctgc gaaaagcggt
      541 aatcctgcgt ggatgctgcc accgccatct gctgaacaat gctgtaaatt ggctgatctg
      601 catcaaaatg accaatacag cccctcaact ccccgtgttt cctcagggta acaaaagcgc
      661 cggctttttg cagcaacgct tccggaattt tcccggagaa tgcttccgga attttcccct
      721 ccccgaggta cacgttgatt gtgttgcggg caatacccag cagttgttct ttttcttttt
      781 ccgaaagctt aaaagtagtt tccttctccg gctggcttgc agtgattgca aaatatccca
      841 ccacgcggtc cttttcaccg tatggcgaat cacctgaatt cctgtaaagt actttattgt
      901 aaacaatatc ctgttcacct tctgtaatat acatcaatgt gagaaccgat ggccaggagc
      961 aacaaccggt cgcgaggttt gccacctgca tcttcataca attgcttatc gcatcaatca
     1021 gtttgtcggg cctgtttgta cagatggcat cggcaatgga catatcaact cttacagcat
     1081 cgttgtatgc cggataatgc gaaaaatctg tgctgatgac aaacagatta tcgcgtgtaa
     1141 aataaggctt cagggcttct gcaattttct tacatatcga cgggtcctgc gttcctaaca
     1201 gtacaggcac tattttataa cctttgctca gataatactg gataaacggc acctgcacct
     1261 ccacgctgtg ctcactttgc tgataaccgg gatcgaaaga aagcgcctta tgctctatgc
     1321 ttaatttccg ggctatttcg aggtcaaccg gagcttcccc aaacggcgtg atatagttcc
     1381 cgatactgta taccgaggct cccgcgaatg agttacgatg gctgcaacca atgataaaaa
     1441 ccgttttata ctcttttgac gggtcaagtt ggttaaaggc cgcggccgtc acctcacccg
     1501 aaaacacata accggcatgc gggacaatta tagccttgat ttcctgagaa ttatggcctg
     1561 ccggggcttt ggcaaacaag gtcttcatcg tttgctgcaa ttcggcagcc gaaccgggat
     1621 aaaactgccc tgccacggcg ggcttccggt tgatgagtgt atcgggtgca ttcataatct
     1681 cggggggatt ggtcgttgga tgacagttta aacaaatgca aagcatgaac actgctgtca
     1741 aaatttgtat ttttaccatg ccgcttttca taggttaaag ttactttaaa tttattagct
     1801 gatcaataag aatatggcat tcctggatct cattactgca cgccagagcg ttagaaaata
     1861 ctcacaaagg ccggttgaac cggaaaaact tgagcgctgt ctcgaagctg cccgactggc
     1921 cccctcagcg agcaataccc agccatggca ttatatcgtg gttcaggatg aacctttacg
     1981 cacaaaagta gcggaagcaa cgttttcggg cccggtaaaa tttaaccggt ttaccctgca
     2041 ggcgcctgtc ctcgtggtta tcctgctcga gcaacccaaa ttacgcaacc gccttgccat
     2101 gatcctgaaa aataaggaat ggaagctgat cgatattggc atcacggcga cgcacttttg
     2161 cctgcaggct gcagaggaag ggcttggaac ctgcatgatg ggctggttca atgaaaagca
     2221 actgaaaaaa gttctttccc tccccattag caaaacgata gggctggtga taagcctcgg
     2281 ttacgcggaa gaagattacc cgttacggac aaagatcagg aagccgatgg aagcaattgt
     2341 gagttataac ggtttttgag gccgggtata agaatgttct aatagcaaat aacgaacaaa
     2401 cgaatttaaa agtcagaaag atacatgcca cctgtttgga taagttgtta accggcaagg
     2461 gtgaatctga caggcatatt gaacctgact cttaccggtt tccctctttg tttgcccggg
     2521 atccatcgcg gcatactctt tataagacgt attgattctt catcgcaacc gcctccaatt
     2581 cctcttaaga cttttacatt cgatatagag ccatctcttt cgacaataaa attgacaaag
     2641 acggttcctt gaatattatt tttctttgca tccggagggt aatgcaaatt ctctgaacaa
     2701 aacttagccc ttgcatccgc ccctcctgga aatgatggcg actcttcaac aactgtaaaa
     2761 acatgataat cgctaacatc agagatttct attgcctcaa tcgagccatc aacttctgat
     2821 atcacatagg ttgtgctgtc cttcccgaat aaatcatgca aaatttcaaa atgagttgct
     2881 cccgggacct ggatttttat ggaatcgggc aaggaaggat taacagtagc gaagcaacag
     2941 attttcttat ataaagtgaa ggcttcatga tcatttaatc ctgcagcatt cttcatatct
     3001 ttacaataat tgcaagtgtc atgtaactga ataaaagata acgccctgtt ataataggca
     3061 tcagcagcac gatataaact cagtgatatc gaatagaact caatggcttt ctcgtattct
     3121 ttttgctgaa agtactcatt acccagatca taatttctgg ttgcttcatt atactggcca
     3181 aaaccgattg tactcagaag tactgaaatc agaattaatt gaaaacgtct catagctgta
     3241 tccaataaat gattatgcga aatagcaata caagatagta aataattgct atataaatga
     3301 aatatttttt atcctcataa acatttcatc gcggacctgc tcataaagag tttttcttct
     3361 aattttacat cgtattcctt tcaaattcaa cccatcggaa atatgaagaa actcattctc
     3421 ctgcctgcat tattgctttt gataatcgca ggctgcaaac agccttccgg caaagccggg
     3481 gctccggtaa catttataaa ccagtctacc atggacaagg taattaagca actcaccgat
     3541 tcctgcggtg aacaggccaa attccgtatc gaacgcggtg tgaaacaggc tgcatcgctc
     3601 tggcagaaag aagacggcac agccgaagaa tttgcaacat tctgcaccga aaatttcgtt
     3661 gccgattcag tcaaattgca gatcctcttc gaaaagctgc agaatgcctt cgaagtactt
     3721 tacggcaact tcaacaaaat gggcatggac ctgcgggttc cggtggacct ggacggtcct
     3781 gaactcactc ccgtggatga gatttttgct tccttctccc cttctgcgca tcttaccgat
     3841 gacctgttcc aaaataagat cgccttcatc accgccctta atttcccgtt ctattcgctg
     3901 gctgaaaaaa ccgaacaggg cgttgcctgg aaccgcaccc aatgggctta tgcacgtatg
     3961 ggagacgtat tcacctcgcg tgtgcctgcc gaattgaatc agaaggtcac cgaagcgctt
     4021 tcgaaaagcg acatgtacat ttccgaatac aacatcatga tgggcaacct ggtgggcaaa
     4081 acgggcaaga cctgttttcc ggctgacatg aaactcatca gccactgggg actgcgcgac
     4141 gaactgaaat cgcattacgg caagccggat gggctcgcca agcagcagat gatctattct
     4201 gtgatgaaac gcatcatcga ccagagtatc ccgcaggacg tgatcaacag caaggagtac
     4261 caatggaatc ccatcgataa taaagtgctg aaaggcagca aagaagttac ctttaaatcg
     4321 gagcctgata cccgttacga gattctcctc aacaatttta aagcgatgaa ggcagtggat
     4381 ccttactacc cggcatatcc cacatacagc caacgcgctt tcgagctggg tatggaaatc
     4441 cctgaagccg atgtggaaaa gctgttcgtc agcctggtca cctccccgga gatcaaacag
     4501 gtgggcgacc tgatcagcaa gaggctcggc cgcaaactcg agcctttcga tatctggtac
     4561 gacggtttta aagcccgcag cagtatcaac gaggacgaac tgacagccaa gacgctcagg
     4621 ctctatcccg atgcaaaagc ttttgaaaag gatatggcca atattctgcg taagctgggc
     4681 tggagcgccg aaagagccgg ttatattgca tcgagggttc aggttgaccc tgcccgcggc
     4741 tcggggcatg cagcaggtgc ctatatgaaa ggcgacaagg cccgcctgcg cacccgcctc
     4801 accgcacagg gaatgaatta caagggatac aacatcgcta cccatgagtt cggacacaat
     4861 gttgaacaga ccatcgacct ttacgatatc gacaattaca tgatgcaggg cgttcccaac
     4921 acggctttca ccgaagcgct ggcatttatg ttccagaaac gcgacctgat gctgttaggc
     4981 atgaaagaca atgatccgaa caaggaacat ctggctgccc tcgacgccat ctggtcaagc
     5041 tacgagatta tgggtgtttc gctggtggat atgcaggtct ggaggtggat gtacgacaat
     5101 cccaacgcca ccccggccca gttgaaagag tcggtgatcc ggatcgctaa agaagtgtgg
     5161 aataaatact atgcaccggt tttcggctca aaggatgaac ccatcctggc catttactcc
     5221 cacatgatcg acaatccgct ttacctttca aactacccgg taggtcacct gatcgatttc
     5281 caggtggaag ggtttatgaa aggcaaaaac ttcgcctccg aagttgatcg catgtatacc
     5341 cagggacgcc tcgtaccgca ggtttggatg aaaggtgccg taggaaacac catctcagtg
     5401 gaacccatgc tgaaagctgt ggatgaagcg ctgaaagtga taaaatagaa gctttaatac
     5461 aggagtattg attcacgcaa aggcgcaggg gaaatccaga gaggaatacc cggtaccttt
     5521 gcgtgattcg tttattttgc catcttctcc ctcgcctctt ccagagttcc cgtaaaaacg
     5581 atccgtccct gcctgttgct ttcccggatg aaatcccgga ggctcttgca tttcacttcc
     5641 gtgaaatctc cgacaattgc cagatgggta tggtagttcg aaaacttttg gagaatgtct
     5701 cccgctatac ctgttttcag atcgaaaaag gcagggataa aattcttctg gtgaagaatg
     5761 atcctgtcgg caccctggta aatacagtta accatcagat ccagtgcatc ctgggtggtg
     5821 cggataagga tttcgtcgga ggtaatttcg gcaatatgag tgtcgtcgga cgggtaaaat
     5881 tctgttttca tggataatat aacttaccgg atctctgtaa atggggataa cctatacctg
     5941 aatttcaaat cccaggtcaa tatcgcttgc agggtggcct ttaatccccc agttcaccag
     6001 atgaggttca ttcagataca caatgatgtc tgtttctgaa atacccggat cgcgtttcag
     6061 gttgtcggaa atcacccggt aaagttttcg tttggcttcc gtacttctgc cttcaaaagc
     6121 cgtgatttcg ataatggtaa agcaatccgt tttatccgta tttctttcga aatcatcttc
     6181 gtccagctcg taaatccgct gattcctgtc gctttccggg atcctgaaag cagataccag
     6241 ggcattgtgg atgccgtcga ggatagcttg tttgtaagct ttatccttcc ctttgtaaat
     6301 ttctaccctt acgagtggca taatgtaaat agcttatggt tttttgtttt gatcctactt
     6361 agttttcagc cgcaaccgtc ttcatatccc caccgtcttg cttctccgct ccagcaacag
     6421 ggtatcccgc cacctgccgt tcattttccc gatcttctcc ctgtaaccta cccgtctgaa
     6481 tcccaaggcc tcatgcatcc tgatactgga atcattttcc ggaaagatgc cggcttgtaa
     6541 agtccatata ccgttttgtc cgctttcccc gatgagtttc tccagcaacc ggctgccgat
     6601 cttctgtccc cggaactttt ctccgacata aacgcttacc tcagctacac cggcataaac
     6661 acagcgctcc gaaaccggcg acagggccgc ccagcctgct atctggccgt tggacagcgc
     6721 aacgaagcgg caggcttcca ggtgacggct gttccactcc tcccacgatg gcactttctg
     6781 ttcaaaggta gcatggccgg tttcgatgcc ctgacggtat atttccgcta catccggcca
     6841 gtccgcagca tccatgggcc ttatcattat catcatatta ggttgcatta tcgggcaaaa
     6901 gtagttgaaa ttgccctgag gcttcaggct aatttttgat tttcggctgg tgagttcttt
     6961 tgtttcgcaa cacaaccgtg agatgaagcc tggtcataat tcgctgccgc tctcaataaa
     7021 ccgtgtcaga tcattcagga accgcaccat cggagcgccg tcgataacat catggtcaat
     7081 gaggattgtc atgttcagga tttcccttac tttgacttca tcgccgataa caacaggttt
     7141 cttcagtacc gagccgatgc caaacgaaag tggatgcacc gttttatgaa tgaaccaacc
     7201 cttgatggtt cccatcatgc ccggcgaagt caccaccaca ttgcccattt tcttgtaggc
     7261 gatctttggg taccgcaaca gaatcctcca gatcctccgt cggaaagctc ccggcaaccg
     7321 gtaataaagc ctttcataaa atttaggctt ttcatttaaa acgatatcat tcttagtcag
     7381 cgccttgtcc tttgcaactt ctatctcccg ggtgatctcn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnng ccagcaggcc cgtaatcaca atctccctgg cattccggct
     7561 cctgagaaaa tccatcagtt ccgctgaagt aaaagcatcg cctgtactga cctttacaaa
     7621 caggttgtcg tttacaatgc tcaggcgggg atcaaagtcg gggacgggca gggtatcggt
     7681 tgaaaatcct ttcgaattga tcaccagaga cttcccagtc gatttgacat acaggattct
     7741 atctttctca aaaccggcga tcagggaatt gatgttggtt atcatgaatc ctgcagcgtc
     7801 ttcgtaaggt ttatccctga tgaattgttc ctgaacgtcg aggaccaaaa gatacttccc
     7861 ctgcgaaaag gaaattgccg ggatcaaaaa caggctgatc aggaaaagtg taagcttcaa
     7921 cgacacttct tgtttcatgg ctatagggat atagggccgg ttctgttccg taaaggaacc
     7981 gataattaca aaaagcaggc aggtcagcga aggcagggat tactcaagtt ctaccaataa
     8041 aattacggta ttttcatttt gccgaaccac cctaaaaccg ttcttcccca acacctttaa
     8101 cgacggtatg ttgtccactg cggtttccgc ccttactttc ctgacggttt tcttctcccg
     8161 aagccattcc atcattcccg cgatagtttc agccatatag ccccggttac ggtaaccctc
     8221 atccgtaccg tatcctatct cgacttcccc ctgctcatcc ggcgctccat ggaaacagat
     8281 gccgccgatg atggcctttt cggcctttcc gattaccagc cacatcgtgt agaagagcgg
     8341 attattggaa gggttgatga tgttcggtaa caagtcattc agaaccgcct ctttggcttc
     8401 ctcctccatc agggattgcg aaggcgccaa gcccagttcc cgggcaaaag cctccggctc
     8461 ttccacgtgt ttcctcagct cttccgctgt caggggctta atatacagcc tttttgtttc
     8521 tatcatgttg tcatgcatgt cttcggatct gttcagcaag agattgccct gctaatttac
     8581 tttctttcta ttcctcagct ttaccgggaa tatcatgggc acctttttcc tgtattccag
     8641 gtattcctcc ccgaaatccc tgatcagtct cttttcttcc gatttcttta aaaacaggat
     8701 ggccaggagc agaaaacaaa acaacacgat caatccggtt atcgaattta aaaagatgac
     8761 caacgaggca tagagcgaaa acgccccgaa aaccatgggg ttccggctgt aacggtacgg
     8821 accggtcgtc accagttttt gggtctgcgg actgatggac acgccgaagg catccgtcgg
     8881 accacccttc cctaaggtga acaggaacag gttcgaccat gccgcaaaga tcgccccggc
     8941 gataaagaac accgcggata aaacatatct caataggtcg gaagaaatca gtaaccggcc
     9001 ctgaaacacg gcatcgcatt gcgacagcct gtaaaaacca tacggtacta acacaatgaa
     9061 cagggtaaat ccgataaggt agcctgcaat aaaacggagg gttcggttca taaagggtcc
     9121 tgtttataag atgtttaaga aggctttgag cctctaatag ggtaagcgtc aggtaattgt
     9181 atatgtattt attcctttat cgcattttca agttctctca ccaattcttc tttatcagga
     9241 agaacatatg tatattttga agcaaacacg ttgttttcca aaccgcctaa aatgtattcc
     9301 gctgcaatct catccttctc tgcacataat attattccta tcggaaggtt gtcatctttg
     9361 tcattaatct ccgttttaaa atagttcaga tatgtattga gttgtcctgc atccttgatt
     9421 tttaattttg tcgatttcaa ttctatcaga atatagctcc gcagaatctt gttgtaaaat
     9481 accatgtcga cgtaatggtg tgtgttatta attgtaattc tttgttgtga tcctacaaac
     9541 ataaaacccc ggcccaattc taaaaaaatt cttcaagatg tcgcaccagc ttttgctcca
     9601 actctttttc caaaatgggt ttatactccg gaattccaag aaattcaaac acgtatggac
     9661 tttttacaat atcctcaggt tttgtaatct gcataccatt ttttgaaagt gcaattatct
     9721 gttctttatt cgtcttacct tttgacagta atagcctttc atagagtgaa ctgtcaatct
     9781 gtctttttag ttcgcggaca gaccaggatg aattaatgga ttctttttca taaaagcccc
     9841 ttttttgaac atcctctata atcagaagtt cgcaaatgtg agaccaggat aattgtccag
     9901 acagtgtctg gacatccgga tattccatgt aaaacttcct catattgaac aggtttgacc
     9961 tggaaaatcc tttcccgaat tctgtagtaa
//
LOCUS       DAOPYV010000097         9623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_8331_length_9623_cov_14.739653, whole genome shotgun sequence.
ACCESSION   DAOPYV010000097 DAOPYV010000000
VERSION     DAOPYV010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8331_length_9623_cov_14.739653"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            571..642
                     /locus_tag="PKI35_10660"
     tRNA            571..642
                     /locus_tag="PKI35_10660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:604..606,aa:Arg,seq:cct)
     gene            679..924
                     /locus_tag="PKI35_10665"
     CDS             679..924
                     /locus_tag="PKI35_10665"
                     /inference="COORDINATES: protein motif:HMM:NF022019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNX44889.1"
                     /translation="MVYVYSLKNEMNAEIYVGITTDIKRRLKEHNTGKNRYTKAFIPW
                     EVFYIEEQPDYMHAREREKYLKSAAGKRFLRRQKDMH"
     gene            1022..1273
                     /locus_tag="PKI35_10670"
     CDS             1022..1273
                     /locus_tag="PKI35_10670"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNX44890.1"
                     /translation="MVYVYSLKNEMNAEIYVGITTDIKRRLKEHNTGKNRYTKAFIPW
                     EVFYIEEQPDYMHAREREKYLKSAAGKRFLVPEKPEPRF"
     gene            1455..2354
                     /locus_tag="PKI35_10675"
     CDS             1455..2354
                     /locus_tag="PKI35_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44891.1"
                     /translation="MSEKLSGNYGYKITIGIMAAVILVLTFLLVSQQRHAASRLQASD
                     EQKFALQDELDSLIAEHQRVKASYGDLSDSLSEKDSIINIKTLEIRKLLNTKYELAQV
                     RQKLEQLRLVTQGYEKQIDSLYTVNRELKTENEQIKINYQHEQVKTADLSKEKEELNK
                     KISSAAILKAYNVRCTAYKAGEAGREKVTDKAARTDKIKVCFTVSENQLVPTSYRRFF
                     VRIARPGDNAILTRGASYTWKYHGETLQFSAMESVSYSGESMDICTYFEKPAGSGELP
                     KGRYIVDVFTDDYAIGQGSLELK"
     gene            2423..3769
                     /locus_tag="PKI35_10680"
     CDS             2423..3769
                     /locus_tag="PKI35_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNX44892.1"
                     /translation="MYAVIDIETTGGSALYEKITEIAIYLFDGQQITGEYSTLINPEK
                     PISPFITRLTGISDEMVQQAPRFYEVARDIVEFTEDCIFVAHNAAFDYGFIREEFRRL
                     GYSYKRPVLCTVKLSRKLIPGFRSYSLGNLCESMGIQIENRHRAAGDARATVEVLRQL
                     IHVDPSLHGVPLNGLNPGLDKDIFSKLPSTAGVYYFYDENGDILYIGKSKDLHSRILS
                     HISNTNGSRAVELRSSIAGISCEETGSELIALLLESEEIKKQLPRFNRAQRQTRYKYG
                     LYSFQNQAGYINLQISKIVASDEPHQVFSSFEEASAFMEALYRKYSLCRKFSGLYSNN
                     GACFDHSIGQCRGACIGAESPEKYNVRAEQALASLRLPFDNMLIIDKGRNDDERSAIL
                     VENGRYVGYGWFNPEYTGNDISSIRECIRTFNDNRDTRQIIRRYLKTNKIRKLIRF"
     gene            3815..5218
                     /locus_tag="PKI35_10685"
     CDS             3815..5218
                     /locus_tag="PKI35_10685"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNX44893.1"
                     /translation="MKRYLLFAFYLFPLAFLVSCRTTTVMPEKPAESYTSFTAQAQPS
                     LINLPVEMKLTNLEALLNKELTGLIYEDADLSDDNLAIKAWKKENIRLTFDKGQFVYN
                     VPLKLWIKAGWKIEKFGISLSDYREVNAEIALKFRTSVGINKDWSLSTQTVSDGYSWL
                     SSPVLKIGPVNVPITFIANLIVKSNMETINSAIDEGMKTSLDLKTKAQEAWVDVQKPM
                     LLSEDYKLWLKITPTAAFASPITVINGTLRQTSAIQGVAECFIGNQPAYTVNSKIPDI
                     SPLSRPNDNFVVNIMSYTPFYYIDSISKAMLLNTSYKFGRKAITITGISVYGSDEKMI
                     VETDVTGSIKGKLFFEGLPAYRAEDSSIVVNNLAFHVQTGSFLLKTANWLYNGGLEKI
                     MQKKMVYAIGPEIQSAFSMIQDNIKHYELGEGFYLNGKLDRFEVLQPGLSKGFVLAPL
                     KFEGKLSVALEEQDGKK"
     gene            complement(5298..5933)
                     /locus_tag="PKI35_10690"
     CDS             complement(5298..5933)
                     /locus_tag="PKI35_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44894.1"
                     /translation="MKTLITITILLILAGKSEAQVSEGTAVFNKAEQPAVTGEFNFPA
                     AVTEQVLLDDLKYRGYGKGYTVKGFHRYEGIIFPDISPEKIDFYFKVNEKGKEKDKSI
                     VTVLVSKGGGAFVSRSGDPAIVDSAKAYLMSLIPAFGKMKLQLDIEEQSEAVARAEKK
                     YKNLVGEGETLVTRLKDLEAGIEKNKQEQEAQKTRLEKEKQLLETLKSRLK"
     gene            6407..6589
                     /locus_tag="PKI35_10695"
     CDS             6407..6589
                     /locus_tag="PKI35_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44895.1"
                     /translation="MKNDVQCGAPSGAVYGLGFIGAAIYFISHAAGFWMGALGFLKAL
                     IWPAFLVYEAFRYFGS"
     gene            7163..7246
                     /locus_tag="PKI35_10700"
     tRNA            7163..7246
                     /locus_tag="PKI35_10700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7197..7199,aa:Leu,seq:caa)
     gene            complement(8491..>9623)
                     /locus_tag="PKI35_10705"
     CDS             complement(8491..>9623)
                     /locus_tag="PKI35_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNX44896.1"
                     /translation="RRREQDRSEDKPFRKEREERPYNRESGPKPYRRREQDRSEDKPF
                     RKEREERPYNRESGPKPYRRREQDRSEDKPFRKEREDRPYNRESGPKPYRRREQDRPE
                     EKPFREERERRSYNREDRPKSFPGRERSGGTSKTSRDGLTRLNKYIANAGICSRREAD
                     ELIQAGVIKVNGKIITELGYKIGPGDRVQYEDQPIRSEKLVYVLLNKPKGYITTADDP
                     FERKTVMTLVEDACKERIYPVGRLDRNTSGLLLFTNDGDLAKKLTHPKHNIRKVYHVE
                     LDKALVKADMMKIAEGVELEDGVAEVDEIAYAEGSASKKEIGIELHSGRNRIVRRIFE
                     SLGYDVVKLDRVIFAGLTKKDLPRGHWRMLTDKEVNYLKMIR"
BASE COUNT         2502 a         2344 c         2270 g         2507 t
ORIGIN      
        1 acaaactggt gaaactgggg tttaaggtat cttaggtatc agggcagctc ttcagactgc
       61 aaattactgc cggcgcttcc gtggaatagt tttcgtgtta attcgtggtg aaacagcaac
      121 cacgaattgc gcgaaaccgg cttcgccggg acacggatta tcacgaaaac cggtatcttg
      181 caattctaat tcgtgtttaa tttgtgttca ttcgtggtga aaaatgatca cgaactaccc
      241 gaaaccggct tcgtcgggac acggattatc acgaaaatcg gtatcttgca attttaattc
      301 gtgctcattc gtgctccatt cgtgaaaatt cgtggcgaaa cagcaaccac taattgcgcg
      361 aaaccggctt ctatgttacc cgttgctttt tgttcttgct gtggctttca ggagatattc
      421 aacaataaaa atcgttgccc tttattgcct cctgaaatat ttgaacgatt ttgtatcttt
      481 gcgcccggta gtaagcagcc ctgtagttca acggatagtc cgtcagggac ggatcctaaa
      541 cttgaaagac taaaagaaat aacagagaaa ggccctatag ttcaacggat agaacggaag
      601 tttcctaaac tttaaatcca ggttcgattc ctggtggggc tacaagaagg ttgtcaaaaa
      661 gaggcaacct tttttgctat ggtgtatgta tattccttga aaaacgagat gaatgctgag
      721 atatatgtag gcattacaac ggatatcaaa agacggctga aggagcataa caccgggaag
      781 aacaggtata caaaagcctt tattccctgg gaagtattct atatagaaga gcagccagac
      841 tacatgcatg cccgcgaaag ggagaagtac ctgaagagcg ctgcgggcaa gcgttttctc
      901 aggcgtcaaa aggatatgca ctagatccag gttccctgcc tgactgccgg cgcagccagg
      961 caggcaggga ttcctggtgg ggctacaaga aggttgtcaa aaagaggcaa ccttttttgc
     1021 tatggtgtat gtatattcct tgaaaaacga gatgaatgct gagatatatg taggcattac
     1081 aacggatatc aaaagacggc tgaaggagca taacaccggg aagaacaggt atacaaaagc
     1141 ctttattccc tgggaagtat tctatataga agagcagcca gactacatgc atgcccgcga
     1201 aagggagaag tacctgaaga gcgctgcggg caagcgtttt ctggttccgg aaaagccgga
     1261 accccggttt taatttgaat tgtttacctg gaaacgttcc tggtaaacgg atacgcttac
     1321 cgaattgtgt acttgtttaa cgaaaccggg aaggtatcaa cgggtacaga attttcttct
     1381 gacgatccat cgtataaatc cgggattccc ttttttgtta cttttgtgta tctatcattt
     1441 ttaatccatt tattatgagc gaaaaattat ccggcaatta cggttataaa atcacaatcg
     1501 gcattatggc cgcagttatt ctggttttga ctttcctgct tgtcagtcag caacggcatg
     1561 cagcttccag gttgcaggct agcgatgaac aaaaatttgc cttacaggac gagctcgatt
     1621 cgctgattgc tgaacaccag cgggtgaaag cctcctatgg cgacctgagc gactcacttt
     1681 ccgaaaagga tagtatcatc aatatcaaaa ccctagagat caggaaatta ttgaatacaa
     1741 agtatgaact ggcccaggta aggcaaaaac tggagcagct ccggctggtg acacaaggat
     1801 atgaaaagca gatagattct ctctataccg taaaccgtga gctgaaaacc gaaaacgaac
     1861 agataaaaat taattatcag cacgaacagg taaaaaccgc cgatctttcg aaagaaaaag
     1921 aagaactgaa caaaaaaatc agcagcgccg ccatactcaa agcttacaat gttcgctgca
     1981 ccgcatacaa ggcaggggaa gccggcaggg aaaaagtaac cgataaagcc gcccggaccg
     2041 acaaaatcaa ggtttgtttc accgttagtg aaaaccagct tgtccccacc tcataccgtc
     2101 gtttcttcgt gcgtattgcc cgtcccggag ataatgccat ccttacgagg ggcgcatcct
     2161 atacctggaa ataccatggg gaaacgctcc agttctcagc catggagtcg gtaagttaca
     2221 gcggcgaatc catggatatc tgcacctact ttgaaaagcc tgccggcagc ggcgaattgc
     2281 caaaagggcg atatatcgtt gatgtattta ccgacgatta cgctatcggc cagggatcgc
     2341 tcgaattaaa ataaataaca tacaggccga tcctgttgcc ccggaaggcc tgacacagaa
     2401 gtttaccgat agaatttacc gcttgtacgc tgttatcgac atcgaaacca cggggggaag
     2461 cgccctgtat gaaaaaatca cggagatagc tatttacctg ttcgacgggc aacagatcac
     2521 gggtgaatat tctaccctta tcaatcctga aaagccaatt tcccctttca tcacacgcct
     2581 taccggcatt tcggatgaga tggtgcaaca agcgcccagg ttttacgaag tggcccggga
     2641 tattgtagaa tttaccgaag attgcatctt tgtcgcccac aatgcggctt tcgactatgg
     2701 ttttatcagg gaagaattca ggagactggg atacagctat aaacgtccgg tactctgtac
     2761 ggtaaaacta tcgcgtaagc tgattccggg atttcgttcc tacagcctgg gaaacctctg
     2821 cgaaagcatg ggaatccaga tcgaaaaccg gcaccgcgct gccggcgacg ccagggcgac
     2881 ggttgaagtc ctccggcagc tcatacatgt tgatccgtcc cttcatggtg ttcccctcaa
     2941 cgggttgaat ccaggcctcg ataaggacat tttctcaaag ctgccttcta ctgccggcgt
     3001 ttactatttc tacgatgaaa acggagatat cctctacata gggaaaagca aggacctgca
     3061 ctcgcggata ttatcccaca taagcaacac caacggttcc cgggctgttg aacttcgtag
     3121 cagcatagcc ggcatttcct gtgaagaaac cggcagtgaa cttattgcac tgcttctcga
     3181 atcggaagag atcaagaaac aattaccccg ttttaacagg gcacaacgcc agaccaggta
     3241 taagtacgga ctgtactcct ttcagaacca ggccggttat atcaacctcc aaatttccaa
     3301 aatcgtcgcc tccgatgagc cgcatcaggt attttcttcc tttgaagaag catcggcctt
     3361 tatggaagct ttataccgga aatattccct gtgccggaaa ttttcggggc tttacagcaa
     3421 caatggggct tgtttcgatc attccatcgg ccagtgccgg ggagcctgca tcggcgccga
     3481 aagcccggaa aaatacaatg tcagggctga acaagccctt gcatccctca ggctgccatt
     3541 cgacaacatg ctcatcatcg acaagggccg caacgacgat gaacgttctg caatactggt
     3601 cgaaaacggc cgttatgtcg gatacgggtg gtttaaccct gagtataccg gcaacgatat
     3661 ctcctccata agggagtgta tccggacttt caacgacaac cgcgataccc ggcaaatcat
     3721 ccgccgctat ctgaaaacca ataagatcag gaagctcatc cggttttgat actaccggag
     3781 cgaatcctgt ttttacatta aaacataacc aacgatgaaa cgctatttgc tttttgcctt
     3841 ttaccttttc ccattagcct ttctggtttc ctgccgtact acgacggtaa tgcctgaaaa
     3901 gccggccgaa tcatatacgt cttttactgc acaggcccag ccttcgctca tcaacctgcc
     3961 ggtggaaatg aaactgacaa atctggaggc cttactgaac aaagaactta ccgggcttat
     4021 ctatgaagat gcagatctgt cggacgacaa ccttgccatc aaggcatgga aaaaagagaa
     4081 tatcaggctg actttcgaca agggccagtt tgtttacaat gtcccgttga aactctggat
     4141 caaggccggc tggaagattg aaaaattcgg catcagcctt tctgattacc gggaagtaaa
     4201 tgccgagatc gcgttgaaat tccgcacttc agtggggata aacaaagact ggtctctttc
     4261 tacgcagacg gtttccgacg gttattcgtg gctgagcagc ccggtgctga aaatcgggcc
     4321 ggttaatgtg ccgattactt ttatcgccaa tctcatcgtc aaaagcaaca tggaaaccat
     4381 caattcggcc attgacgagg gaatgaaaac gagccttgac cttaaaacca aggctcagga
     4441 ggcctgggtc gacgttcaga agcctatgct gctttcggaa gattacaagc tctggcttaa
     4501 aataaccccg acagccgcct ttgcatcgcc gattaccgtg atcaacggca ccttgcgaca
     4561 aacgtcggcc atacagggcg tggcggaatg tttcattggg aatcagccgg cgtataccgt
     4621 caattcaaaa attcccgata tctcgccgct ctcccggcca aacgacaact tcgtggtgaa
     4681 catcatgtcg tatacgcctt tttattacat cgattccatt tcgaaagcca tgcttttaaa
     4741 caccagttac aaattcggca gaaaagcgat caccattacc ggcatttcag tttatggcag
     4801 cgatgaaaaa atgatcgtag aaaccgatgt gactggaagc attaagggaa agttgttttt
     4861 tgaaggtctg cctgcttacc gtgcggaaga ttcgtctatc gtggtcaaca acctggcctt
     4921 tcacgttcaa acgggcagct ttctgttgaa aaccgctaac tggctctaca acggcggcct
     4981 ggagaagatc atgcagaaaa agatggtcta tgccatcggc ccggaaatcc aaagcgcctt
     5041 cagcatgata caggacaata ttaaacacta tgaattgggc gaaggtttct acctcaacgg
     5101 caaactggac cgtttcgagg ttttgcagcc gggcctctcc aaaggatttg tactggcccc
     5161 gctgaagttt gaagggaaac tttcggtggc gctggaggag caggatggaa agaaatgatc
     5221 ttccgccaac cgccctgaaa cacattcagg agaataggct ttcccgctcc tgccgatgat
     5281 ggattcgcct ccggacttta cttcagccgg cttttcagtg tttcgaggag ttgtttttcc
     5341 ttctccagcc gggttttctg agcctcctgt tcctgcttgt ttttttcaat gccggcttca
     5401 aggtctttga ggcgggtaac cagcgtttcg ccttcaccca ccaggttctt atactttttt
     5461 tcagccctgg ctaccgcttc ggactgctct tcgatatcaa gctgcagttt catctttccg
     5521 aatgcgggga tcaggctcat gaggtatgct ttggcagaat ccacgatggc cggatcgcct
     5581 gaccgggaaa caaatgctcc gcctcctttg ctgaccagga ccgtgacgat gctcttgtct
     5641 ttttctttgc ccttttcatt taccttaaag taaaagtcga ttttttcagg actgatatcc
     5701 ggaaaaatga tgccctcgta ccggtggaat cccttaactg tatagccttt tccatagccc
     5761 cggtatttaa ggtcatcgag caacacctgc tcggtaaccg ccgcaggaaa attaaactcg
     5821 cccgtaaccg ccggttgttc agccttgttg aagacggctg ttccctcgct gacctgggct
     5881 tccgatttcc ctgccagaat gagcaggatg gttattgtaa tcagggtttt catggcgaaa
     5941 agtttaaaaa gaatcgtgca ggcttaattc ttattccaaa gataatcaat aagatgggtg
     6001 tttcaaattg tttcgggtag ttattccggc gtggtcttat tatttatttc cgcagaagcc
     6061 cgggaaaatg gtttttgcag acgccggcag tagctatcag gattggttac atttttatgc
     6121 ttgttgtgga ggaattaaaa tcaatcgatg atttgaacgc ttccattcgg taaaaaatct
     6181 cagaaagcca tcatacggcc atcttcgcaa aggtagtgac agtccacagt gttgacactg
     6241 gcttaaagaa cctcctttgg cccagattca gcaccggaca attcctgatg tctgcaatcc
     6301 cttaataatt gaaccgcaag gaaaatgaaa atggcagata tcactcttaa ccattgatca
     6361 aataatgtaa ttttactctt atgattcacc ttttaaatgt caaaatatga aaaacgacgt
     6421 tcagtgcggg gcgccttcgg gcgctgtgta tggtttaggc ttcattggcg ctgccattta
     6481 ttttatttcc catgcggctg gtttctggat gggtgcgctt ggctttctta aagcattgat
     6541 ctggcctgcg ttcctcgtat atgaagcctt cagatatttt ggcagttgat aaaaacaagc
     6601 ctgtcaacct gtttatcgca tatcccggaa cctcagacag cttaatatgt atgatacgtt
     6661 tgccggattg gttccgccag ttaatgatcc gggcaaaagg tacctgtttc cggaagctgg
     6721 cttattgtca tctgataaaa ggattaaaag ggtaagaaac ctctgaaacg gacttatacc
     6781 aatttatatg tcagaacagc gcaaagaatc cagccggtga aatgaaacga attatccggc
     6841 atatcatcct gatcctgctc attctgcatc cgtccgattc attttcaaat gacgggaaaa
     6901 aggcggataa gctgttttcg ttgccggaaa tcaggtttta agacgacaga ttcttccggg
     6961 agatcatgtg gcgcagaaaa atgaattccg gagctgatgg ccggtaaaaa tccgtcacgc
     7021 ggaagagcgc cgccggaaag aatcttataa ggaaggctat ggcattaaag atctgataat
     7081 tattacggtc taaggtcaaa atgcatcaaa acaagacagc cgataaaatt gaattccgtt
     7141 ttttatatct ttgcaccttc aagcccaggt ggcggaattg gtagacgcgt cggtctcaaa
     7201 aaccgatgga cgtgagtccg tgccggttcg atcccggccc cgggtactta ctgaaagcgc
     7261 aaatgcctgt aaagcttttt ttacaggcat ttttcttata aaaatacata caagatattc
     7321 cggttcaatt actaaaccgg tattctgttg agcaacacac tgtccaaaat gttgactagt
     7381 ttcacctcag ccggctgtcg ctggcaggga ctgccggcct gttttcatcc gggatactca
     7441 atcagtaaat tgcatatgga tactgtctcg ccggtgctgc caaaataacg gtgaaaccct
     7501 gaaggtttcc aaaaccttca gggtttgccc cgccggtgtt cctgctcctt gagcgcctgt
     7561 cccgattcat cggggagagg cgccggaggc gcatgaggtg ataataaaag cccccgcacc
     7621 taaggtccca tcagggaaaa tacaagtatt cacgcccgcc gttcactgac taccggcctg
     7681 gtttcgtccg ggaaaaccaa tcagtacatt gtttcaggat accacccctc cggtactacc
     7741 aaaataacag tgaaaccctg aaggtttcga aaaccttcag ggtttgcccc gccggcgttc
     7801 ctgctccttg aacgccaggc cctattcatt gggaagaggc gccggaggcg gatgatgtaa
     7861 taataaaagc ccccgcacct aaggtcccat cagggaaaat acaggtattc acgccccccg
     7921 ttccttgact gccggcctgt tttcatccgg gatactcaat cagtaaattg catatggata
     7981 ctgtctcgcc ggtgctacca aaataacggt gaaaccctga aggtttccga aaccttcagg
     8041 gtttgccccg ccggcgctcc tacttcttga gcgctggtcc cgattcatcg gggagaggcg
     8101 ccggaggcgg aagcaatgcc gggggcgcca ataataagaa gtaacccgac gcactaggac
     8161 tccggattca ttctcagggt ttcccggaga tggtccacca ggtacaggtg aaaaatgggg
     8221 ctgtcacgga caaacctgaa ccgtaaacgg cgtctgaaag ttcggcgctt taaacgtttt
     8281 gactcagttt ttcagtatct gagatacaat tgaaaaaata tggatacaac gaatgatgac
     8341 cagttttcct taagcagttg ttatggttgt tcttcattct ccggaattat cctgctacaa
     8401 cttgaaaatt gtggtttgta ccggaccggg gaacaaaaaa gcctgcagag aaatcttccg
     8461 caggcttttc tattcctgta gcgttttatt ctaccggatc atcttcagat aattcacctc
     8521 tttatccgtc agcatgcgcc aatggccgcg cggaaggtct tttttcgtca ggccggcaaa
     8581 tataaccctg tcgagtttta cgacatcata gcccagcgat tcaaaaatac gcctgacaat
     8641 gcggttgcgc cccgaatgca gttcaatgcc gatttccttt ttgctggccg agccttcggc
     8701 ataggcaatt tcatctactt ctgctacgcc gtcttccagt tccacccctt cggcaatctt
     8761 catcatatcg gcttttacca gggccttgtc gagttcaaca tgatacacct tgcgaatatt
     8821 gtgtttggga tgggtcagct tttttgcaag gtcgccgtcg ttggtaaaca acaacaaccc
     8881 ggacgtgttg cggtcgaggc gtccgacagg ataaatgcgc tccttgcagg catcttcgac
     8941 cagcgtcatc accgttttcc gttcgaaagg atcatcagcc gtggtaatat aacctttggg
     9001 cttgttgagc aacacataaa ccagcttctc gctccggatg ggctggtctt catactgcac
     9061 cctgtcaccc gggcctattt tatagcctag ttcagtaatt atttttccgt tcaccttgat
     9121 aacgccggcc tgtatcagtt catccgcttc gcggcgcgag cagataccgg cattggcgat
     9181 gtatttgtta aggcgggtaa ggccgtcccg ggatgtcttt gacgtcccgc cggatctttc
     9241 ccgcccggga aatgatttgg gacgatcttc acgattatac gaacgtcttt ccctctcctc
     9301 gcggaagggc ttttcctccg gacggtcctg ctctctcctt cggtaaggct tgggaccgga
     9361 ttcccggttg taaggccggt cctccctctc cttgcggaag ggtttatctt cggatctgtc
     9421 ttgttctctc ctgcggtatg gcttgggacc ggattcccgg ttgtaaggcc gttcttccct
     9481 ctccttgcgg aagggtttat cttcggatct gtcttgttct ctcctgcggt atggcttggg
     9541 accggattcc cggttgtaag gccgttcttc cctctccttg cggaagggtt tatcttcgga
     9601 tctgtcttgt tctctcctgc ggt
//
LOCUS       DAOPYV010000098         9435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_8565_length_9435_cov_10.442217, whole genome shotgun sequence.
ACCESSION   DAOPYV010000098 DAOPYV010000000
VERSION     DAOPYV010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9435
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8565_length_9435_cov_10.442217"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PKI35_10710"
     CDS             complement(<1..856)
                     /locus_tag="PKI35_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44897.1"
                     /translation="MLDRFQLWEMNSDGNINWTKQVLPGTQTYFSGTEFQQMCFTSDG
                     GSLLAGYTYYPYDTTNNPWDGILQPCLVKHDSLGNRQWVYPPISGADTNHIGFFNACV
                     QVGNTYYAAGSNYNYGPGHFLQPLVARFDLNGNLLSYVSSGPDTMYHELTQILPATDT
                     SILLISAANNAFGDPNYLMVFIANTLGNFNKGFIRHDLIVGQYGDEVCIMRDNKFIIP
                     CSFPIGGISANTDIVAIKLNANLEYDSIYTQQFTYDSLCPYPIVSDTVICNCEPFVSA
                     EEAESVTEA"
     gene            complement(1590..3203)
                     /locus_tag="PKI35_10715"
     CDS             complement(1590..3203)
                     /locus_tag="PKI35_10715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44898.1"
                     /translation="MKMKATEIQMGSDCARIRRQARAGRRLTIIIVFLMTLSPVSGSA
                     QAVYFNNRYNLYPFNTWDYSKTILEVEDGYIISGATGGDNNLHNMALTKLNKLGEVVW
                     SKYMSDFDNSYSPGYSGSLLKYSEDVYFGAGIARSYTSNWVYDRGLLIKYNSEFDTLW
                     KKEYGETSAPFDTSYLFRNLTFALDGGIIMTGSWVEYSTEIMNAHAYLIKTDTSGNKL
                     WEHVYTNLPGDVQGYSVISTTDGGYAIGAFQYYHVPPSDETGDPLVIKTDSLGNFEWV
                     KNLGSEYSDSQDMLALSTDGNILVYCTYAVLNNPGSDSYKSPQVTKLDNNGSIIWERR
                     YLKPKHFTYPGNLYVDADGSIILCGKTMVSFPRNTGWMMKLTSDGDSLWYRQYSLMIG
                     NDSEHMLYDVIPASDNGYIACGYVNPVLPDTGTQDVWVVKVDSMGCESPTFCWVGIEE
                     QQVAPKMGELLAFPNPAKDFFRVQFQQVSQKSLLQFVDMTGRKAEETNITPFTTTEVL
                     NCSHWPPGVYFGQLICDGKIVAKGKVVVK"
     gene            complement(3494..4327)
                     /locus_tag="PKI35_10720"
     CDS             complement(3494..4327)
                     /locus_tag="PKI35_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44899.1"
                     /translation="MISKKNLIALSVSMIIAGTALGQESLNPEFRYEKGKSYRYSLTN
                     NSSFIQEMMGQENKGNTMLTQQGTLNINDVDEKGVMTGTFTWEDFSTRTKVMQTDTVL
                     SFDSYKGKTCDISLTGKGKMRITRSDSTVIIQGAPVTPVNTMLQFMSLPEHPVAKGDT
                     FLVKTIDTAYDANNNPTITEISRKYVYERRMMAPKDILCYVFGFEGTVASSGNIETMG
                     VKMYQETEGTEKGELLLSENGILVAVHSSNEKEMTMAVSGQMGMTMSMTLHETADYRL
                     E"
     gene            complement(4346..5920)
                     /locus_tag="PKI35_10725"
     CDS             complement(4346..5920)
                     /locus_tag="PKI35_10725"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNX44900.1"
                     /translation="MKTLRSALFFLLLYSLLMVPGYSPAQKPDKHALKSITPALMKEY
                     IGFLASDALAGRNTPSPGLDTAAAFIARTFRANNVAPLNGNYFQPFYLCKTDLDTGCY
                     LQIVKEGKISDIPLKTGFIPYYLSNSKVFTGEAVFAGYGITAPEYQYDDYAGLDVKNK
                     AVLVFRHEPCDNDSGSFFEGREPTRYSGVIYKQETAEKQGAGALLVVSEPGAYPSLKP
                     RGYIWPSLSKNFPADAAPYTLCDNENKDIPAIQIGAEAIKSIFGSIDSLRSLQTRIDK
                     SRFPHPLQLPGVEIRLKINARNDQRQVVNVVGYLEGSDPVLKNEIVVIGAHYDHVGIN
                     DLKKTGEDNIFNGADDNASGTSGVMALARAFAMSKNKPRRSMLFLLFAGEEKGLYGSA
                     VYTAHPLLPLEKTVAMLNLDMIGRNSPKTLYLVGASTSPDIAAITKKENRKTGFKLID
                     DNSVMGGSDHYNFFEKGIPFMFYFTGLHKDYHQVGDNPDKINYEKAARVTRLVYLTAW
                     HIANESRHYKLNTSKN"
     gene            6125..9334
                     /locus_tag="PKI35_10730"
     CDS             6125..9334
                     /locus_tag="PKI35_10730"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNX44901.1"
                     /translation="MKNFRKLNNLLGWITFAIASVVYLLTIEPTASWWDCGEYISTAF
                     KLQVGHPPGAPLFQLIGRFFSLFAFGDVTKVAKMVNIMSAFSSSFTILFLFWTITMLG
                     LKLVNEEEMTAGQGLAILGSGLVGALAYTFTDSFWFSAVEGEVYAMSSFFTAIVFWAI
                     LKWDRVHDQPHALRWILLIAFLVGLSIGVHLLNLLTIPAIGLVIYFRKYKANWKGIVY
                     TLIISFFVLAFIMWWLIPWTVALAGKYEIAFVNNLGLPFNTGTIFYFVLLIGTLVWGV
                     RFTYRDNHTIAEYIVGGAMLLLLSIFSWISFLAFLAVVGAYLWYRQSSTAGSKRNQLR
                     SLVNTGILSIAFMLIGYSSFFMLVIRANANTPINENDPSDAVSLLSYLNREQYGSWPI
                     FYGQYYNAPLDPQHPMKDGSPIYVKDLKTGKYKITDDRKNSIYNYDKRFCTLFPRMWS
                     SDESRRHPQNYKSWAKIKGVPITATDDEGKTQTIMKPTFGENLRFFFRYQIGHMYMRY
                     FMWNFSGRQNDIEGHGGIRYGNWISGIPFIDNARLGDQTDLPDSMDNRARNKFYMLPL
                     ILGMIGMIYHFSKRSDGALLVLLLFFMTGIAIVMYLNITPQQPRERDYAFAGSFYAFA
                     IWIGLGVMAIWDSLRKIKSKWMAVVITLVCLLLVPGIMAKEGWDDHDRSGKYACRDFA
                     ANYLNSCAPNAILITNGDNDTFPLWYAQEVEGIRTDVRVVNFMLASGDWYVHQLKRKI
                     YNSDLLPMTLRYDQYDKGVNEGVVYSGGYNERIELQELIDYIASDDPSTKYQYGNRPV
                     SVFPTKKVKMTVDTTNMVRNGIVPKELANKMVTEIEWDIKGNYLFKNDLMLLDFLAKN
                     NFRRPVYFASPSSLSDVVNVDKYCFLDGFVYRFLPVKNDSLDYIPGLGSVNSEASYDI
                     LMNKCKWGNLADPKVYVDRESYRNSLMPRQNFARLARKLCQEGKYDKAVKVCDYVQKV
                     FPDDKIRFDYYMIQFIESYYIAGAFDKGNKLAEKLIRTYEQNTGYISSLSPEFRANYD
                     EDLNQAFAFFGAMQQLADQYKQVKISKQVETFLNRTMGK"
BASE COUNT         2389 a         2136 c         2220 g         2690 t
ORIGIN      
        1 aggcttcagt aacgctttcc gcttcctctg ccgatacaaa cggttcgcag ttgcatatta
       61 cagtatctga tactatgggg tacgggcaca ggctgtcgta agtgaattgt tgtgtgtata
      121 tgctgtcgta ctcaaggttg gcgtttagct tgatggcgac tatgtcggtg ttggcactta
      181 ttccaccaat aggaaaagaa catggaatta taaatttatt gtctctcatg atacatacct
      241 catccccata ttgccctacg atcaaatcgt gtcggataaa gcccttattg aagttgccta
      301 aagtattggc aataaaaacc ataaggtaat tggggtcgcc aaaagcatta ttagcagctg
      361 atatcaacaa tatggatgta tccgttgccg gtagtatttg agtaagttca tggtacatag
      421 tatcaggccc tgaactaaca tagctgagca ggttgccgtt gaggtcgaac ctggctacca
      481 ggggctggag gaaatgcccc ggcccgtaat tatagttgct tccggccgcg taataggtat
      541 tgccaacctg cacacaggca ttaaagaagc ctatgtggtt ggtatcggct ccgcttatgg
      601 gcggatacac ccattggcgg ttgcccaggc tgtcgtgctt cactaagcag ggttgcagta
      661 tgccgtccca ggggttgttg gtggtgtcgt agggataata ggtataaccg gccaacaggc
      721 taccgccatc ggaggtaaag cacatctgtt ggaattcagt accgctgaag taggtttgcg
      781 tacccggcag tacttgtttt gtccagttga tgtttccatc ggaattcatt tcccaaagtt
      841 ggaatcggtc caacataata tttaaactgg aagcagccgt gacaaaaata atttgatcat
      901 cagatttaat tttaataaat tttaacaggt tttcagagtt aaatatattt tttttgcacc
      961 agacgaattc gccgcaatta tttgtcttaa cgatataagc ataaaacagg tttccttcaa
     1021 aaataacacc accttgaaat attattcctt catgcgatga tgaaatgcta aatggaccga
     1081 tataattgtt ttcatagtat agatattttt tcaaccataa aatattacca ttgatatcag
     1141 ccttaagaac attcgcacca tcaccattaa agttaattat aaatccattg tcataagaat
     1201 tgacggtgca tactgcaatt tcagcatggc caggtacacc atatgtacgt tgccacattt
     1261 gagctattga gttaacactg ataatagtca gggctaatag tagaaccgtt tgtttcataa
     1321 aaagaaaaaa aatgaaaggc agagattaag aggctctgcc tttccggtca acggcactta
     1381 acgtaccaca ttaaacttaa ctgcagcttt tatattgccg tttacccaca agcaacaagt
     1441 ataagctccc gaatgcgatt tgccggtttg ccatataatt tggtcagcag ttttcaattg
     1501 ccccatctcc tgtatagccc gccccgcttg cgcgcgtccc ctgacgcgtg cgcgggaatc
     1561 tttcgttatt atttctattt gtttcatttc tattttacca ccactttccc ttttgccaca
     1621 attttgccat cgcatatcaa ttgcccgaag tacacgcccg gcggccagtg ggagcagttg
     1681 aggacttctg tagttgtaaa gggtgtaatg tttgtctctt ctgcctttct gccggtcata
     1741 tctacaaatt gcagcaggct tttttgcgaa acctgttgaa actgaaccct gaaaaaatcc
     1801 tttgccggat taggaaatgc taataattca cccatttttg gagctacctg ctgttcttct
     1861 atgcccaccc agcaaaaggt aggactttcg caacccatac tatccacttt taccacccac
     1921 acatcctgcg tgccggtatc gggcaacaca gggttaacat aaccgcaggc aatgtagccg
     1981 ttgtcggagg cgggtatgac atcatacaac atgtgttcgc tatcgtttcc gatcataagg
     2041 ctgtattgac ggtaccacag actgtctccg tctgatgtta atttcatcat ccatcctgtg
     2101 tttctcggga aagaaaccat tgtttttcca caaaggatga ttgagccatc tgcatcaaca
     2161 taaagatttc ccggataggt aaaatgctta ggtttgaggt atctcctttc ccaaatgatg
     2221 gaaccattat tatccagttt agtaacttgg ggacttttat atgaatcgga cccgggatta
     2281 ttcaacactg cataggtaca ataaacaagg atattaccat cagttgataa tgcaagcata
     2341 tcttgcgaat cactatattc gcttcccaga ttctttaccc actcaaaatt gcccaggcta
     2401 tcggttttta tcaccagggg gtcgccggtt tcgtctgaag gtggcacatg gtagtactga
     2461 aaagcgccta tggcataacc gccgtcggta gtggatatta cgctgtaccc ttgtacatcg
     2521 cccggcaggt tggtatatac atgctcccaa agcttgttgc cggaagtgtc ggtcttgata
     2581 agataggcat gggcattcat tatctctgtt gaatattcaa cccaggaacc tgtcataata
     2641 atgccaccat ccagggcaaa agtcaggttt ctgaaaaggt atgacgtgtc aaaaggcgca
     2701 gatgtttcac catactcttt cttccaaagt gtgtcaaact cgctattata ttttattagc
     2761 aaccccctat catataccca atttgaagta tatgacctgg caatgcccgc accaaaatat
     2821 acatcttcag agtattttaa caaagagccg gaatatccgg gtgagtaact attatcgaaa
     2881 tcagacatgt attttgacca tacaacctca ccgagcttat taagttttgt caaagccatg
     2941 ttgtgcagat tattgtctcc acctgtagca cctgaaataa tataaccatc ctcaacctca
     3001 agaatagtct ttgaataatc ccaagtattg aaaggatata ggttatagcg attgttaaaa
     3061 taaaccgctt gagcacttcc tgatactggc gataaggtca tcaagaatac gataattatt
     3121 gtcaatcttc tcccagcccg cgcttgtctt cggatgcgcg cgcaatcact tcccatctgt
     3181 atttctgttg ctttcatttt catttcacca ccactttact ccccgccacc ggtttgcccc
     3241 ggtacaccag ccggaactgg tacatgcccg ccggcaggcg cgaaacgtcc gcctgaacgg
     3301 gtgcgcctgt atggatactg cctgctgcaa cggcgatatc atcgccctcc cgctgatcag
     3361 tttctcagcg tcaatctgtt tctcctttta gcgtattccc aacctgcctg ccgtaaagga
     3421 gatgtctcag ggaaacatcc acagaccgga tttcgcggag cggggtacaa caggcttaag
     3481 ccgttccgtt atcttattcc aatctgtagt ctgccgtctc atgcaaggtc atggacatgg
     3541 tcatgcccat ttgtccggat acggccatgg tcatttcttt ttcattggag gaatgaacgg
     3601 ctaccagaat gccattttcg ctcagtaaaa gttctccttt ttcagtacct tccgtttcct
     3661 ggtacatttt cacgcccatg gtttctatat tgccggaaga agccacagtg ccttcaaaac
     3721 caaacacata gcacaggata tcctttggcg ccatcatgcg tctttcatag acgtatttcc
     3781 tcgatatttc cgtaatcgtg ggattattgt tcgcatcata agccgtatcg atggtcttta
     3841 ccaggaaagt gtcgcccttt gcaaccgggt gttccggaag gctcatgaac tgcagcatgg
     3901 tattgacggg tgtgacaggc gctccctgga tgatgaccgt tgaatcggat ctcgtgatcc
     3961 gcattttacc ctttccggta agcgagatgt cgcaggtttt ccctttatag ctgtcgaacg
     4021 acaggactgt atcggtttgc atcactttgg tgcgggttga aaaatcttcc caggtaaaag
     4081 tgcccgtcat cacccctttt tcatctacat cgttgatatt cagggttccc tgttgggtca
     4141 gcattgtatt gcctttgttt tcctgcccca tcatttcctg gataaaagaa ctgttgtttg
     4201 ttaaggaata ccggtagctc ttgccttttt catacctgaa ttcggggttc aggctttcct
     4261 ggcccaatgc cgttccggct attatcatac tgacagatag tgcgataaga ttttttttcg
     4321 atatcataat ggttgaattt ggttgttaat tttttgaggt attgagtttg taatgtcggc
     4381 tctcgttggc aatatgccag gcggtaaggt acacaaggcg cgtgacgcgg gcggcctttt
     4441 cgtaattaat tttgtcagga ttgtcgccaa cctggtggta atccttgtgg aggcctgtaa
     4501 agtagaacat gaagggaatg cccttttcga aaaagttgta atgatcgctg cctcccatta
     4561 ccgaattatc gtcgatgagc ttaaaaccgg ttttccggtt ttcttttttc gtgatggcgg
     4621 caatatccgg gctggttgaa gccccgacaa ggtaaagcgt cttcggggag tttcgcccga
     4681 tcatgtcgag gttcagcata gccacagtct tttccagagg cagcagcgga tgggccgtgt
     4741 aaacagccga tccgtaaagc cctttttcct cgccggcaaa cagcagaaaa agcatgctgc
     4801 gcctgggctt gtttttcgac atggcaaatg cgcgcgccag ggccattacg cccgaagtgc
     4861 ccgaggcatt gtcgtcggcg ccgttgaaaa tattgtcttc tccggttttt ttaagatcgt
     4921 tgatgccgac atgatcgtaa tgggccccga taactacgat ttcgtttttg aggacaggat
     4981 cgcttccttc cagatagccg acaacattaa cgacctggcg ctggtcgtta cgggcgttta
     5041 tttttaacct gatttcgacg cccgggagct gtaaaggatg ggggaaacgg cttttgtcaa
     5101 tacgggtctg gagcgaccgc aagctgtcga tactcccgaa gatgcttttt atggcttcgg
     5161 cccctatctg aatggcaggg atgtccttgt tttcgttgtc gcataaggta taaggcgcgg
     5221 catcggcagg gaaattcttg gagagggagg gccagatgta gccgcggggt ttgagcgacg
     5281 ggtaggcgcc gggttccgaa accacgagca gggcccccgc tccttgtttc tccgcggttt
     5341 cctgtttata gatcaccccg gaataccggg ttggctcccg gccttcgaaa aatgatccgc
     5401 tgtcgttgtc gcagggttcg tgcctgaaaa cgaggacggc tttgttttta acatccaggc
     5461 cggcatagtc atcgtactga tattcagggg ccgtgatgcc gtatccggca aataccgctt
     5521 ctccggtaaa taccttggag tttgataaat aatagggaat aaaacctgtt ttgagcggga
     5581 tatcggatat ctttccttct ttcacgatct gcaggtaaca accggtatcg agatcggttt
     5641 tgcagagata aaacggctgg aaatagttgc cgttgagagg ggcgacgttg tttgccctga
     5701 aggtccgggc aataaaggca gccgccgtat caagccccgg gcttggcgtg ttgcggccgg
     5761 caagggcatc ggaagccagg aatccgatat attccttcat caatgccggc gtgatgctct
     5821 tcagggcatg tttgtccggt ttctgggcgg gggaatatcc cggaaccatg aggagggagt
     5881 aaagcagaag aaaaaataat gctgatcgaa gggttttcat ttatggatga cgctgtttgc
     5941 cggtgttatt taacattcaa aaatacgaag aaaggcgtta ccaggcagcc ggaatattaa
     6001 taactataat aattttgtta atacttgtta agcctgattg ctaagctctg aattaagttg
     6061 caaattattc atatttcctt aatttcgcag cataattcag aataacccat ttaataaatt
     6121 attcatgaaa aacttcagga aactgaataa cctgcttggc tggataacct tcgccatagc
     6181 atcggttgtt tatttactga ccatcgagcc aacggccagt tggtgggatt gtggtgagta
     6241 tatttcaaca gcttttaaac tgcaggtggg gcatcctccc ggagcgcctt tgttccagtt
     6301 gataggccgg tttttctcat tgtttgcttt tggcgatgtg acgaaggtag ccaaaatggt
     6361 aaacatcatg tcggcctttt cgagcagctt taccattctt ttcctgttct ggaccatcac
     6421 catgctgggc cttaagttag tgaacgaaga ggaaatgacg gcagggcagg gcttagccat
     6481 tctcggaagc ggtctggtag gcgctttggc ttatactttc actgattcgt tctggttttc
     6541 ggccgttgaa ggcgaagttt atgccatgtc gtccttcttt accgccatcg tgttctgggc
     6601 gatcctgaaa tgggaccgtg tgcacgacca gccccacgct ttgcggtgga tattgctgat
     6661 tgcgtttctg gtaggactat caattggcgt tcaccttctg aacctgctga ccattccggc
     6721 aatcgggctg gttatctact tcaggaaata taaagcgaac tggaaaggca ttgtatatac
     6781 gctgatcatt tcattttttg tgctggcgtt tatcatgtgg tggctgatac cctggacggt
     6841 agcgcttgcg ggcaaatatg aaatcgcttt tgtgaataac cttggcttgc ctttcaatac
     6901 cgggacgatc ttttattttg tcctgctgat cggaaccttg gtatggggcg tcaggtttac
     6961 ctaccgcgat aatcacacga ttgctgaata tattgttggc ggggcgatgc tgttgctgct
     7021 ttccatcttt tcatggatca gtttccttgc tttccttgca gtggttggcg cctatctttg
     7081 gtacaggcaa agttcgactg ccggaagtaa aaggaaccag ttacgttcgc ttgtcaatac
     7141 gggaatactc agcattgctt tcatgctgat cggctattca tcatttttta tgctggttat
     7201 cagggccaat gccaatacgc ctatcaatga aaacgatccg agcgatgccg tcagccttct
     7261 ttcgtatctg aacagggaac aatacggctc atggcccatt ttctacggcc agtactacaa
     7321 tgcgcccctc gatccccagc atccgatgaa agacggttcg cctatttatg tgaaggatct
     7381 gaaaaccggc aagtataaaa ttacggatga ccggaagaat tccatctaca attacgataa
     7441 aaggttctgt accttattcc cccgtatgtg gagttcagac gaaagccgcc gccatcccca
     7501 gaactataaa agctgggcta aaatcaaggg cgttcctatt accgccacgg atgatgaagg
     7561 aaagacgcaa acgataatga aacccacttt cggggagaac cttcgtttct tcttcaggta
     7621 ccagatcggt catatgtata tgcgctattt tatgtggaat ttctccggaa ggcagaacga
     7681 cattgaagga catggcggta tacgttacgg gaactggatt tccggaattc ctttcatcga
     7741 taatgcgcgc ctcggagatc agacggatct gcccgacagc atggacaacc gggccagaaa
     7801 caagttctat atgctgccgc tgatactggg gatgatcggg atgatttacc atttttcgaa
     7861 acgttccgat ggggcattgc tggtcttgct gttatttttc atgacaggca ttgccattgt
     7921 gatgtacctt aacattaccc ctcaacagcc gcgtgaacgg gactatgcgt ttgccgggtc
     7981 gttttacgcg ttcgcgatat ggataggatt gggcgttatg gcaatatggg attcgcttcg
     8041 caagattaag agcaagtgga tggccgttgt cataaccctg gtttgcctgt tgctggttcc
     8101 cggaataatg gccaaagaag gatgggacga ccatgaccgt tcagggaaat atgcctgccg
     8161 cgattttgcc gcaaactacc tgaattcgtg tgcccccaac gccatcctga ttacaaacgg
     8221 cgataatgat accttcccgc tctggtacgc ccaggaagtt gaaggcatca ggaccgacgt
     8281 gagggttgtt aatttcatgc tggcatccgg cgactggtac gttcaccagt tgaaaaggaa
     8341 aatctataat tcggatctgc taccgatgac cctccggtat gaccagtacg ataaaggcgt
     8401 aaacgaaggg gtcgtataca gcggcggcta taatgagcgc attgaattac aggaactgat
     8461 cgattacatt gcaagcgacg atccttcaac caagtaccag tatggcaacc gtccggtcag
     8521 tgttttcccc accaagaagg taaaaatgac cgttgatacc acaaacatgg tcaggaatgg
     8581 tatcgtgccg aaagaactgg caaataaaat ggtcaccgaa atagaatggg atatcaaggg
     8641 gaattatctg ttcaagaatg atttgatgct gcttgatttt ctcgccaaaa acaatttcag
     8701 gaggccggtg tattttgcca gcccgtcgag cctcagcgat gtcgtgaatg tggataagta
     8761 ttgtttcctt gatggattcg tttatcgttt cctgcccgtt aaaaacgatt cgcttgatta
     8821 tattcccgga cttggcagtg ttaattcaga agcatcgtat gatatcctga tgaataaatg
     8881 caaatgggga aatcttgccg atccgaaggt ttatgttgac cgtgaaagtt atcgcaactc
     8941 gctgatgcca cggcagaatt ttgccaggct ggcccggaaa ctttgccagg aaggcaagta
     9001 tgataaggct gtcaaggtgt gcgattatgt tcagaaggtg ttcccggatg ataaaatccg
     9061 cttcgactat tatatgattc agtttattga atcatattac atagcaggcg cttttgacaa
     9121 aggcaacaag cttgccgaaa aactcatcag gacctatgag caaaacacgg gttatatatc
     9181 ctctcttagt ccggaattca gggctaacta tgatgaagac ctcaaccagg cgtttgcttt
     9241 ctttggagcc atgcagcaac tggccgatca gtacaagcag gttaaaatca gcaaacaggt
     9301 tgaaactttc ctgaacagaa ctatgggaaa atagtctgta tcggagataa aaccctgtat
     9361 aataaaaaga ggctgtccgg aaagggaggg cactgaaaaa gtatctctta ttaaattaat
     9421 ccttagaaat aagtt
//
LOCUS       DAOPYV010000099         8981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_9208_length_8981_cov_11.168721, whole genome shotgun sequence.
ACCESSION   DAOPYV010000099 DAOPYV010000000
VERSION     DAOPYV010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9208_length_8981_cov_11.168721"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PKI35_10735"
     CDS             complement(<1..743)
                     /locus_tag="PKI35_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX44902.1"
                     /translation="MGTTAPTYALITGASAGIGREFAKELATRKYNLVLVSLPGTGLG
                     QQGESLKKRFKTDVRIIETDLMVQGNHEKVKEYILSENLPLSLLINNVGLGHNGEIGT
                     YTTRKINEMVLLNVATTTLMTNLLVPELKKSPRAYILNMGSFGGFMPLAYKSIYSATK
                     SYVYHFSVSLAAELKGTGVQVSVALPGPVPTNDLVKDRIDKSGRIAREIILPADRVAA
                     FTLEKMFAGKQVIIPGRGNRMMYYFGNLIP"
     gene            complement(748..1737)
                     /locus_tag="PKI35_10740"
     CDS             complement(748..1737)
                     /locus_tag="PKI35_10740"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX44903.1"
                     /translation="MKCFITGANGFIGSRLAEKLTAEGHQVTCLVRSPEKALIFNGWS
                     PRHIIGDLHDTGTLMRGMEGCDHVFHLAAYARPWSKDKSLPYKINVDGTVNVLEAALR
                     AGVSKVVVTSSAAVTGPSGDRGFVNEDSVRIAPFFNEYEETKALAETRVKEYAVKGLH
                     AVIVNPTRVFGPGPLNQSNSVTKMLRGYYRGTWHIMPGDGCKTGNYVYIDDVVNGHIL
                     AALNGISGEKYILGGENVSFRQLFEKFAGATGRRRLLVPLPVGIMIGIANFLEFQYKI
                     TSIPPPITAPWVNKYLENWNLSSEKAVRQLGYSITPLDQAIGQTLLWLKNSKP"
     gene            complement(1734..2438)
                     /locus_tag="PKI35_10745"
     CDS             complement(1734..2438)
                     /locus_tag="PKI35_10745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNX44904.1"
                     /translation="MPGITATITAFILTILFLLVKPVVVRGYPRSGSKTLHLISGFSL
                     VKLSGVVLFGVMPFLVLLSSGIDPAAYGLQKGNNAGYWYLFICLPLLIVTISAFASRN
                     KEMLNLYPDLKFRTWTPGRLTVLTAGWLLYLAAYEFLFRGFLFFSCFYSFGFTAAAII
                     NIVLYSLAHIPKGLKETAGAIPFGLLLCWIAWLTGSIWLPLIAHLSLALSTQYFAIYH
                     HPEMKFCWSVKSSKIL"
     gene            complement(2761..4995)
                     /locus_tag="PKI35_10750"
     CDS             complement(2761..4995)
                     /locus_tag="PKI35_10750"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HNX44905.1"
                     /translation="MKKLTLLVICLVSSLFLSAQEKMYIHKSDNMTLGALISTIDSIY
                     FSNDGTITYFRIDSNLAQYPTAQIDSLSFGDNSNTIFVTYNTSGVSVINPLAFEGVSV
                     SVEGQHVTVNSTSETQDINYSLSGTTQNGMFKIYSDKRFNLLLNGVSITNQDGPAINV
                     QSKKKTSVELIAGTTNYVTDGATYAPPAINGNGEEEDQKGTFFSEAKLVFSGTGSLTI
                     EGNGSEQHALCSDDLIQVDGGNITVTKAVVDGIHGKDGIEITGGAVNVTASGDAIDGD
                     EACILISGGTVTTNNTQADVKGISCDSTLTISGGTLDLTISGDQSKAIQSNQAMTLSG
                     GSITIHNAGNVVLAASGSGFDPSYPSSLKSDSLITISGADITITSTGKGGKGITSNTG
                     IAISGGNLTITNSGNGERYKNSSGVYKAYVASCLNSDGYIAITGGTVTTTSSGSGGRG
                     ITPDGALTIGSTTTSPTVSITTTGSDVFISGSGNNASYAEAKAITGNGDIIFESGTVT
                     IASADDGIKSETSVTFNNADVTISNSYEGIEAPFITVNGGSVKSAASDDSFNATFGNG
                     GEFDDGSLLKIQGGTVVANASTGDGLDSNGSIMITGGTTIVHGPQSAPEVGMDFNGTC
                     NVNGGFLVISGINSNMTQPPSPTSSQHSVLAKTNNALSSTTLFHVQDDAGNDILTFQP
                     ARNYSSVIFSTSSLQTGVTYSIYKGGTSTGTNNNGLYTGGTYSGGTFKKSFTISNTIT
                     TVTF"
     gene            complement(4992..5474)
                     /locus_tag="PKI35_10755"
     CDS             complement(4992..5474)
                     /locus_tag="PKI35_10755"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44906.1"
                     /translation="MRRLLLLTNFFLCMIIIPGLRAQGLIIRLHDGTENSRLITSVRK
                     LSFPDNNLVVTPKTGSPDTFPLSEIQKVYFGEVVSVPEPLSSDKENLHLYPNPSDKEI
                     SILNIPEGTSAVYIYKMDGRLVRQATVASGTVTINIEDLTSGIYFLIAGDRTAKFIRL
                     "
     gene            5886..7745
                     /locus_tag="PKI35_10760"
     CDS             5886..7745
                     /locus_tag="PKI35_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNX44907.1"
                     /translation="MKFTTCIAAFLTLTVLLLNRPVYSGVPDDPAAVSSIVLLKPSGK
                     EFKGFPVLEVMPSGDPGTGVMKELLENSFLREMAALHQLVENDLSGKGIISVKEPMYL
                     LASGRMGGFPMNGFFLKTGKKLIDKRNVPYVDLMNLNQNYKRLGSVTQIFPHEQAHLY
                     FRLFSGIDPEKIESFSSDVHYFSITTNYLTAFNEGFAESFENISRVFETNPAISGAVR
                     KDTTRLKSFLGSRIKGFDRDFRWPGRLGLYRLTMLLWYQDLEDYKRYAWSLDGSARFW
                     PLTLNGSPVEKRLLYRNAGVLPDKRGTRSSAGCSSNEGVINAFFTQLMISHAGNCNRQ
                     DSSLSPVQNQLMKEFNVIYKYLRGIPPGASLFDAFISGYITEYPSEKQIILNVYRQVC
                     GFDFTGNAAPEIWLFNPDHRHNPMVMAQFGGCSIPYYTMNLNTAQAEDLMTFPGVDKA
                     KADEFVACRDSSGFFQSIGDLEKLPARLHETGILLKGAVLHPHDLDKYNDEESFSLKG
                     LFTSILNRFLMLNGFLFLGIFSLTFLLFGYRRKPVYRSMGMAARALFKSILFLLAVVG
                     IFFLPYPPVYLFLAFIAVILLINILRTRNNPAKRKEIVFSSALLGVLIIYSLI"
     gene            complement(7843..8616)
                     /locus_tag="PKI35_10765"
     CDS             complement(7843..8616)
                     /locus_tag="PKI35_10765"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX44908.1"
                     /translation="MSNETKSIHEFDFSLICEYFSAIERQGPGSPEVTVQALGFIDNL
                     TDESRIADLGCGTGGQTMVLAQHAPGQITGIDLFPAFIDLFNLNAAKLNLNDRVKGII
                     GSMDNLPFQEEELDLIWSEGAIYNIGFKRGLNEWRKFLKKGGYLAVTEASWFTAERPA
                     EIEEFWHDAYPEIDTIPNKVMQMQDAGYIPVSTFILPENCWTEHFYVLQAAAQETFLK
                     KHAGNKAAEDLVANERHEARLYNRFKEYYGYVFYIGKKI"
BASE COUNT         2163 a         2115 c         2323 g         2380 t
ORIGIN      
        1 ggaataaggt tcccgaaata gtacatcatc cggttgccgc ggccgggaat gatcacctgc
       61 ttcccggcaa acatcttttc cagggtaaag gcagcaaccc tgtcggcggg taaaatgatc
      121 tccctggcga tcctgcccga tttgtcaata cggtctttta caaggtcgtt ggtggggacg
      181 ggcccgggca atgctacgct tacctgtacg ccggtgcctt ttaattctgc tgccagtgaa
      241 acggagaaat gatagacata cgacttggtg gcgctgtaaa tgctcttata ggcaagcggc
      301 ataaacccgc caaaacttcc catattaagg atataagccc tggggctttt ctttaactcc
      361 ggaaccagca ggttggtcat cagggtggtg gtggctacat taagcaacac catttcgttg
      421 attttacggg tggtataagt gccgatttcc ccgttatgtc ccaggcccac gttgttgatc
      481 agcagactca acggcagatt ctccgaaaga atatattcct tcactttctc atggttacct
      541 tgaaccatga ggtcggtttc aatgatcctt acatctgttt tgaagcgttt tttcaacgat
      601 tcgccttgtt gccccagccc ggtcccgggc agtgacacca ggaccagatt gtatttcctg
      661 gttgccagtt ctttggcaaa ttccctgccg atccctgcgc tggcgccggt aatcagggcg
      721 taagtcggtg ctgtggttcc catacggtca tggttttgag tttttcagcc agagcagggt
      781 ctggccgatg gcttgatcga gcggggtgat ggaatacccc aattgcctga cggctttctc
      841 gctggaaagg ttccagttct caaggtattt attcacccag ggtgcagtga taggcggcgg
      901 gatagaagtg attttatact gaaattcaag gaaattggca atgccgatca tgatgccgac
      961 gggaagggga accagcagcc gacgccggcc ggtagcgccg gcaaattttt cgaacagttg
     1021 ccggaatgaa acattttctc cccccaggat atatttttct ccggaaattc cgttcagcgc
     1081 cgcaaggata tgcccgttca caacgtcgtc gatataaacg tagttgccgg tcttgcagcc
     1141 gtcgcccggc ataatgtgcc acgtgcccct gtaatatccc cggagcatct ttgtcaccga
     1201 attgctttgg ttcagcgggc cggggccgaa tacccgcgtg ggatttacaa tcacggcatg
     1261 aaggcctttt acggcatatt ccttaaccct ggtctcggcc agggccttgg tttcctcgta
     1321 ttcattaaag aacggcgcta tccgcaccga atcttcgttt acaaaccctc tgtcgcccga
     1381 tgggcctgtc accgctgccg aggaagtaac aacgactttt gatacgccgg cccgcagggc
     1441 agcttcgagg acgttcaccg tgccgtcgac attaatttta tatggcaacg atttgtcctt
     1501 tgaccagggc ctggcataag cggccagatg aaaaacatgg tcacagcctt ccatgccccg
     1561 cataagtgtt ccggtatcat gaaggtcgcc gatgatgtgc ctgggagacc aaccgttgaa
     1621 aatcagcgct ttttcgggcg acctgacgag gcacgtcacc tggtggcctt cggcggtaag
     1681 cttttcggca agccttgaac ctataaaacc attagcgccg gtaataaagc atttcataaa
     1741 attttgctgc ttttaacgga ccaacagaat ttcatttcgg gatgatgata aatggcaaaa
     1801 tactgtgttg agagcgccaa tgacagatgc gcgatcagcg gaagccagat ggaaccggtt
     1861 aaccaggcta tccagcaaag taaaaggccg aacgggatgg cgccggcagt ttcctttaat
     1921 cctttcggaa tatgggcaag cgaatacagg acgatattga taatggctgc cgccgtgaaa
     1981 ccgaaagagt aaaaacagga gaaaaacaga aatccccgga agaggaattc gtaggcggca
     2041 agatagagga gccatcctgc cgtcaggaca gtcagccggc ccggcgtcca ggtcctgaat
     2101 ttcagatcag ggtacaggtt cagcatttcc ttgttgcgcg aggcaaaagc gctgatggta
     2161 acaatcagca aaggcaaaca aataaaaaga taccagtagc cggcattgtt gcctttctga
     2221 agcccgtatg ccgccggatc aatacccgac gaaagtaaaa ccaggaacgg catcactccg
     2281 aacaatacaa cgcctgataa ttttacgagt gaaaaacccg aaatgagatg cagcgttttg
     2341 cttccggacc ttggataacc gcgcactaca accggtttaa caaggagaaa gagaatggtg
     2401 aggataaaag cagtgatagt tgcggtaatg ccaggcatat ttcctccggt tttttaagta
     2461 aatatagtat atatatttcc tgtcaccaat atattattgg aaatttattt tttagcctga
     2521 aaaaacttta ttggattcgg ctgccgtaaa gaaaaccagg gtccccggct tgatgaaacc
     2581 gcgcaaacaa agccggggat tactttctct tttgtgcgaa ggcgaccgtg acgggaattg
     2641 atttccggct gcataccctg ccacgtctga aaatgggtaa aacagaaaag gcctcctttt
     2701 caggacagcc tttccatgta agggccggtt tcatatcgac ggcggaaaaa attgcccggg
     2761 ctaaaatgtc accgtcgtaa tggtattgct gatggtaaac gacttcttga aagtgccccc
     2821 ggaataggtt cctcccgtat aaaggccgtt gttgttggtc ccggtagagg tgccgccttt
     2881 ataaatggaa taggtgacgc ctgtttgcag ggaagacgtg gagaaaatga cggaagaata
     2941 gttgcgggcg ggctgaaaag tgagaatgtc gttgccggca tcatcctgaa catgaaacag
     3001 cgtcgtggaa gacaaagcat tgtttgtttt tgccagtacg ctatgctgtg acgaggtggg
     3061 gcttggcggc tgcgtcatgt ttgaatttat tccggagata accagaaatc ctccgttgac
     3121 attgcaggtc ccgttaaagt ccatccctac ttctggggcc gactggggcc cgtgaacaat
     3181 cgttgttcct ccggttatca tgatgctgcc gttgctgtcg aggccgtcgc cggttgaggc
     3241 attggcgacc acggttccgc cctgtatttt cagcaggctc ccgtcatcaa attcaccccc
     3301 gtttccgaaa gtggcattga agctgtcgtc ggaggcagcc gacttaacac ttccgccgtt
     3361 cacagtaata aaaggggctt cgatcccttc ataggaattg gaaatggtaa cgtcggcatt
     3421 attgaaggta acagaggtct ccgatttgat gccgtcatcg gccgaagcga tggtgacggt
     3481 cccgctttca aaaatgatat cgccgttgcc ggtaatggct tttgcttcag cataacttgc
     3541 gttattgccc gatccggaaa taaatacgtc gcttcctgtg gtggtaatgc tgacggttgg
     3601 cgatgtagtt gtgctgccga tggtcagggc gccgtcgggg gtaatgcccc ggcctcccga
     3661 ccctgaactc gtggtggtga ccgttccgcc ggtaatggcg atatacccgt cggaattcag
     3721 gcaggaagca acataagctt tatacactcc tgacgaattc ttgtatcgct cgccattgcc
     3781 ggaattggta atggtaaggt tcccgcccga aatggcaatt cccgtgttcg acgtgatgcc
     3841 tttccctcct ttgccggtac ttgtgatcgt gatatcagcc ccgctgatgg taattaaaga
     3901 atccgatttc agggaggaag gataggacgg atcaaagccc gatcctgagg ctgccagcac
     3961 aacatttcct gcgttgtgaa tggtaatgga gccgccgctc agcgtcatgg cctggttgct
     4021 ttggatggct tttgactggt cgccggaaat agtcaggtcg agggtgcctc ccgagatggt
     4081 gagggtgctg tcgcacgaaa tgcctttcac gtcagcctgg gtattgttgg tggtaacggt
     4141 tcccccggaa ataaggatac aggcttcgtc gccgtcgata gcgtcgcccg aagcggtcac
     4201 gttgacggcg ccgcctgtaa tttcaatgcc gtccttgcca tgtatgccgt caacaacggc
     4261 tttggtcacg gttatattcc cgccgtcaac ctgaatgagg tcgtcgctgc ataaggcatg
     4321 ctgttcggat ccatttcctt cgatggtgag cgatccggtc ccgctgaaaa ccagcttggc
     4381 ttcgctgaaa aaagtccctt tctggtcttc ctcttcgccg ttgccgttga tggccggagg
     4441 cgcataagtg gccccgtcgg tcacataatt ggtggtgccg gcgataagtt ccaccgaagt
     4501 cttctttttc gactgaacat tgatggcagg gccatcctga tttgtgatac ttaccccatt
     4561 cagcaacagg ttaaatcttt tatcgctgta tatcttgaac atcccgtttt gggtggttcc
     4621 tgaaaggctg taattgatat cctgggtttc ggaagttgag tttaccgtga catgctggcc
     4681 ttcaaccgaa acggacactc cttcaaaagc cagcggattt atgacggata cgcctgaagt
     4741 gttataagtg acaaagattg tgtttgaatt gtccccgaac gaaagactgt cgatctgggc
     4801 ggtgggatac tgggccaggt tgctgtcgat ccggaaatag gttatggtcc cgtcgttgct
     4861 gaaataaata ctgtcgatcg tcgaaatgag cgctccaagg gtcatgttat ccgatttgtg
     4921 gatatacatc ttttcctgtg ctgacaggaa caggctgctg accaggcaga tcacgagtaa
     4981 ggtaagtttc ttcatagcct gataaattta gcagtcctgt cgccggcaat caggaagtaa
     5041 ataccgcttg tgaggtcttc aatattgatg gttacggttc ctgaggcgac ggttgcctgc
     5101 ctgacgagcc gcccgtccat cttatagata taaacagccg acgtgccttc gggaatgttg
     5161 agaatgctga tttctttatc agacggattc gggtacaggt ggagattttc cttgtcggac
     5221 gataaaggct caggcactga aactacttca ccgaaataga ctttctgaat ttccgagaga
     5281 ggaaaagtat ccggcgaccc tgttttggga gtaacaacga gattattgtc cgggaaactg
     5341 agcttccgga ccgaagtgat cagccgggag ttttcagtcc cgtcgtgcag cctgattata
     5401 agtccctggg cccgcaaccc gggaattatt atcatgcaaa ggaagaaatt cgtcagcagc
     5461 aacagccttc tcattacatt tggtttttgg ttgaaagtac agatagttgg tttgatttta
     5521 tcgggtgacg ttatgaacgg ttctgtaatt cctgtacatt gtttttttgc cgggacgggg
     5581 taactgcctg gtgttatttc cggcggagca tagtaagaaa aatcgtgaca gggtgcggcg
     5641 caggaaaaaa tttgcagttt aatcaaaaac gtcggcaaag gtaaacagat tgctaccgcc
     5701 gtttgaggtt aatagaccaa caggcttttt ttgcagacag aaaccataaa cggggaggat
     5761 ggatgccctt ttaataatat tgtaaccggc atcaggcatt ggatacgtta aacaaaatct
     5821 ggctttccgg tgtaaccatt tcctctgatg ccagtcttat caacatactt tctggattta
     5881 cggctatgaa atttacaact tgcattgctg cgtttttaac gttgaccgta cttttactga
     5941 acagacctgt ttattccggc gtgccggatg atcccgctgc cgtttcctcc attgtgcttc
     6001 tgaaaccttc gggaaaagag ttcaaaggat tcccggttct tgaggttatg ccatccggcg
     6061 atcccgggac aggggtgatg aaagaactcc ttgaaaacag ctttctcagg gaaatggccg
     6121 cactgcatca gcttgtcgaa aacgatctgt cggggaaggg aattatttcc gttaaggaac
     6181 cgatgtacct cctggcctcc ggcaggatgg gcggcttccc gatgaacgga tttttcctga
     6241 aaacgggcaa gaaactgatc gacaaacgga acgtgcccta tgtcgatctc atgaacctga
     6301 atcagaatta taagcggtta ggctccgtca cgcagatatt tccccacgaa caggcccatc
     6361 tctatttcag gctgttttcc gggatagatc ctgaaaagat cgaaagcttt tcgagcgatg
     6421 tgcattattt cagcataacg accaattatc ttacggcgtt caacgaaggc tttgccgagt
     6481 catttgaaaa tatttcaagg gtttttgaaa ccaatccggc tatttccggg gcggtgagga
     6541 aggatacgac gcgtttgaaa tcctttctcg gaagcaggat aaaagggttt gaccgggatt
     6601 tcaggtggcc cggaagattg ggcttgtaca ggcttaccat gttgctgtgg taccaggacc
     6661 tggaagatta taaacggtac gcctggtctt tagacggatc ggcccgtttc tggccgctga
     6721 cgctgaacgg ttccccggta gaaaaaaggt tgctttaccg gaatgccggt gtgctgccgg
     6781 acaaacgcgg gacaaggagc agcgccgggt gttcgtcgaa cgaaggggtg atcaatgcgt
     6841 tttttacaca attgatgatc agccatgcgg gaaactgcaa ccggcaggat tcttccttat
     6901 cgcccgtgca gaaccagctc atgaaagaat ttaatgttat ctacaagtat ctgagaggta
     6961 tacctccggg cgcgtccttg tttgatgctt tcatctcggg ctatatcacg gagtatcctt
     7021 ccgaaaaaca gatcatcctg aatgtctatc gccaggtgtg cgggtttgat tttaccggga
     7081 atgccgctcc cgaaatctgg ctctttaacc cggatcacag gcataatccg atggtgatgg
     7141 cacaattcgg gggctgctcc attccttatt ataccatgaa cctgaacaca gcacaggcag
     7201 aggacctgat gacatttccc ggggtggaca aagccaaagc tgatgaattt gttgcctgcc
     7261 gcgattcttc cggatttttt cagagcatcg gcgacctgga aaaactgcca gcccgtttac
     7321 atgaaacggg aatccttctg aaaggcgctg tccttcatcc ccatgacctt gacaagtata
     7381 atgacgagga aagtttttca ttaaagggct tattcacaag catcctgaac cggtttctga
     7441 tgcttaacgg atttttattc ctggggattt tttccctgac cttcctgctg tttggttacc
     7501 gcaggaaacc ggtttaccgg tccatgggaa tggcggcaag ggctcttttt aaaagcatat
     7561 tattcctttt ggccgttgtc gggatatttt tcctgcctta tcctccggtt tacctgtttt
     7621 tagcttttat cgccgtcatc ctgctgataa acattctcag gacaaggaat aatcccgcga
     7681 aacggaaaga gattgtgttt tcttcggccc ttttaggtgt gttgataatc tattcgctga
     7741 tttaaaatgc attgaaaaaa aaagccggct ggaagccggg cttttaaaaa agtgagaagg
     7801 attgccctga taaaccgggg acaatccttc cgctatgatc gtttaaatct tctttccgat
     7861 gtagaacaca tagccgtaat actccttaaa cctgttgtac aaacgggctt catgccgttc
     7921 gtttgctaca aggtcttcgg cggctttgtt gccggcatgt ttcttcagga aagtttcctg
     7981 cgctgccgcc tgaaggacat agaaatgttc ggtccagcag ttttcaggca ggataaaggt
     8041 agagacggga atgtatccgg cgtcttgcat ctgcatgact ttgttgggaa ttgtgtcgat
     8101 ttccggataa gcatcatgcc agaattcttc gatctcggcg ggcctttccg cggtaaacca
     8161 cgatgcctcc gtaacggcaa gatatcctcc ttttttcagg aatttccgcc attcattcag
     8221 gccgcgtttg aaaccgatgt tatagatggc cccttccgac cagatgaggt ctaattcttc
     8281 ctcctgaaaa ggaaggttgt ccattgaacc gatgatgcct tttaccctgt cgttcagatt
     8341 gagtttagcg gcattcaggt taaacaggtc tataaaagca ggaaacaggt caatgcctgt
     8401 aatttgtccc ggcgcatgct gcgccagcac catcgtctgt ccgcctgttc cgcagccgag
     8461 gtcggcaatg cgcgattcat cggtcaggtt gtcgatgaag cccagcgcct gaactgttac
     8521 ttccgggctt ccgggtccct gtcgttcaat ggctgagaaa tattcgcaga ttaaactgaa
     8581 gtcgaattcg tgaattgatt tagtttcgtt actcatggtg tttaaatttg ttttgccggg
     8641 gccatgacga ttgaagacgc catgcctttg ccatgttccg gcttgttatt gtaatgggaa
     8701 aaagattcag cccggcacag aaatgccggg tgttgaacag aggtataacc cggggcagaa
     8761 agccgggggt tattacttca cacgctccga aatcaattta aacatccaaa gtttttagaa
     8821 ttggcaaaga taagaatctt ttgatattct tttcaatctg ttgcgccttc cgatcattat
     8881 tacttcccac tattgtccgg ataaaacagc catggattgt ataaaatatt gaacatgtgt
     8941 tgattaagcc cctctccttc aggagagggg ttggggtgag g
//
LOCUS       DAOPYV010000100         8905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_9327_length_8905_cov_12.018644, whole genome shotgun sequence.
ACCESSION   DAOPYV010000100 DAOPYV010000000
VERSION     DAOPYV010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8905
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9327_length_8905_cov_12.018644"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PKI35_10770"
     CDS             complement(<1..263)
                     /locus_tag="PKI35_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44909.1"
                     /translation="MQRVIWITLILLLAACSRKAGNQVIIPDEPPSEVIDHPAVVVYA
                     HGNKSMIREKTDAVNAARLISELQIPVITTKNKNLEFVKRNGDR"
     gene            complement(335..1198)
                     /locus_tag="PKI35_10775"
     CDS             complement(335..1198)
                     /locus_tag="PKI35_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44910.1"
                     /translation="MKRLVPYLLLCMLVFSSRNARCQEEDKYCGWTFGLNFGLYYPGK
                     YTANYYNGSNSNENTASYVFGNYYWYQEIYNALDASDTVFIAANGLPANMHYKVSMNP
                     GLFAEYRFNKDYALNAEFNYLKLKTSDAVSIEVDPKEYATEADIRLYPIKGIEERVYF
                     NLSLKRYFESQRKNVMYFALGGININNTKVLKNFLYIGGKEYSIINVYGRQSYIPNSN
                     MQAYNVYEGGIGYGLVAGGGASIEFGNGIILEPGITANWTKVNLEGYKRFTPGLGIYV
                     RFILNSLLLAE"
     gene            complement(1289..2563)
                     /locus_tag="PKI35_10780"
     CDS             complement(1289..2563)
                     /locus_tag="PKI35_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HNX44911.1"
                     /translation="MLTRLYNSIKNIDRKDYRPVFGGLEILKYIGPGLLVTVGFIDPG
                     NWASNLAAGSAYGYKLLWMVTLSTIMLVILQHNVAHLGIATGLCLSEAATKFTGTFVS
                     RSLLTSAMAASVATSMAEILGGAIALQMLVGIPVKAGSIILMITICILLFTNSYRRLE
                     KWIIGFVSVIGLSFIYELSLTNINWMEAATGWVKPSIPKGSMIVVMSVLGAVVMPHNL
                     FLHSEIIQSRQWNLQDETVIRKQLRFEFLDTLFSMIIGWAINSAMILLAASAFFGNHI
                     RVSELQQAQGLLQPLLGNHAALIFAVALLFSGIASAITSAMAGGSIFAGFYGEPFDIK
                     DNHSRLGALLSLVIAVLVIFLTGDPFKGLVISQMLLSIQLPFTVFFQVHLTSSQKVMG
                     KYANSAFYTAMLLLIGSVITYLNVMLFIDLIS"
     gene            complement(2704..3183)
                     /locus_tag="PKI35_10785"
     CDS             complement(2704..3183)
                     /locus_tag="PKI35_10785"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HNX44912.1"
                     /translation="MDSPNQLRIVSGGQTGVDRAALDAAMAAGIPCGGYCPLHRRADD
                     GIIPLRYPLTETSSKEYPVRTRMNVEQSDGTLIITTGILDRGTRLTENICRHKLKPLL
                     TIDLTSDYDIPRVTGWIKISGILVLNIAGPREMSSPGIYRAALAYLNLLFSYLKRGL"
     gene            complement(3367..5457)
                     /locus_tag="PKI35_10790"
     CDS             complement(3367..5457)
                     /locus_tag="PKI35_10790"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNX44913.1"
                     /translation="MKKYTLFLATLIIGLSAMAQNPFLEKQWNTPFGVPPFNRIKYEH
                     YMPAIEQGIKMHNDEINAIVNSKQPPTFANTIEALEKSGNILYRVRTVLSNLESANTS
                     DELQEINRESSPKLTEHYDNIYLNEKLFARVKAVYDNRSKLKLKGEQAKLLQETYKNF
                     VRNGALVSEDKKPRLREINARLAILDIEFGNNVLKATNGYYMTIDNAGDLKGLPQNII
                     NAAADAARNKGLDGKWIFGLNKTEVIPFIQYAENRALRRQLYQYYLNRCNGGKYDNNP
                     LIDETVALRGEKAAIMGYPDYASYVLDDNMAKTPENVYKLLNQLWTPALEMAKREQSD
                     IQAMINKEGNTFTVQPWDWWYYSEKVRNEKYNLSDEELKPYFEVSNVREGVFNTAHKL
                     YGLNFKKRPDVPVYQKDVDAYEVTDASGKLIGLLYLDYFTRPEKRSGAWMTNYIDQRI
                     ENGKDIRPVVSLNYNFPAPSAGEPALINWDDVTTFFHEFGHGLHGLLSKCTYASLSGT
                     AVPRDFVELPSQIMENWASEPEVLKVYARHYKTGEVIPESLIKKMQAADKFNQGFATT
                     EYLAASILDMDYHVVPAGKEIHAQCFEKHSMDKIGLIDAIPPRYNSTFFQHIFSGGYS
                     AGYYAYIWAEVLDADAFSYFSKNGIFNKATADSFRKNILERGGTDDAMKLYKQFTGGK
                     EPSVEPLLERRGLK"
     gene            complement(5586..7742)
                     /locus_tag="PKI35_10795"
     CDS             complement(5586..7742)
                     /locus_tag="PKI35_10795"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNX44914.1"
                     /translation="MALEDFTSNHKLLHSYKYNWAPIEKGYNRRTLHVNVGTGAIREK
                     PVSQMMIEKFTGGKGFGLKLLWEETKPGTKWDDPENEIVINTGPICGITQYSGSGKSL
                     VVTISPQTDIVIDSNVGGYFGPFLKFAGFDSLELQGKAGEDVIVLIDETREVVEIYEA
                     GNESPDSHLLAEYLTEILASTEKDRVNIGVVSAGTAADNSLIGMLNFSFYDVRRKLVR
                     LKQAGRGGIGTVLRNKKIKAVVAHSKGVTGNRNNVVDLEAIMERGKRFNKEMRELDDK
                     QCEMRAKGTAHLTHIMNDYDLLPTHNFKFGSHPDAHNIRGDVWKSYFTQGMPDGCWIG
                     CNMACAKGVDNYELRTGPYKGSKVIVDGPEYESTSSLGSIAGIFNPDYTIEANFYCDT
                     YGICTISWGTIMGFIMECYENGILNEERTGGLKLNFGNADSAMELLHRVSRGEGFGKI
                     AGLGVHKMKQIFAENGWGDAQFLQDIGMENKGLEYSQYVSKESLAQQGGYAMTNKGPQ
                     HDEAWLIFMDMVNNQIPTFEKKAEALHYFPMFRTWFGLQGLCKLPWNDVEPENNAQSG
                     EPAKVPEHVDNYVTIYKAVTGNTNFDKVELIRQSERVYNFQRVFNIRRGYGLRKNDAQ
                     PYRAAGPVTEEEYLSRQERYDKQLIDLMNLDPSTMTLAEKMKATRAYREDRYEKLLDA
                     VYKRRGWNSNGVPTIEHMKNLGMDLPEVLEVITPLQ"
     gene            7966..8577
                     /gene="thiF"
                     /locus_tag="PKI35_10800"
     CDS             7966..8577
                     /gene="thiF"
                     /locus_tag="PKI35_10800"
                     /inference="COORDINATES: protein motif:HMM:NF006395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HNX44915.1"
                     /translation="MTSEEIMMILKGKKAGIAGCGGLGSNCAVALARAGVGALVIVDF
                     DVIDESNLNRQYYFRDQIGMLKVTALKNNLERINPFIDISAVELRLSPPDILKYFSGC
                     DVIVEAFDDAGMKLMMIDTVLNQMPEKFLVTGVGMAGWGDNNSIRTRISDKLVICGDE
                     TNEVSADVPAIAPRVGVVANMQANAVLEILLGKNPFQAIRTSF"
     gene            8626..8829
                     /gene="thiS"
                     /locus_tag="PKI35_10805"
     CDS             8626..8829
                     /gene="thiS"
                     /locus_tag="PKI35_10805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNX44916.1"
                     /translation="MKIILNNREEILDQHSLTISELLKVKNFSFKMLVVKINGNLVRK
                     NEYDITRVNDGDDVMVLHLISGG"
BASE COUNT         2193 a         1984 c         2264 g         2464 t
ORIGIN      
        1 cgatcgccat tccttttgac aaactccagg tttttgtttt tcgttgtgat aaccggaatt
       61 tgaagttcag agatcagacg cgcagcgttt accgcatctg ttttttcccg gatcatactc
      121 ttgttaccgt gagcataaac aacaacggcc ggatgatcaa taacttccga tggcggctcg
      181 tcagggatga taacctgatt cccggctttc cgcgaacatg ctgccagtaa aagaatcagt
      241 gttatccaga taacccgctg catcgcttct ttattcctgc caaattactt taaaacctat
      301 cagaaaaaca gggtattagc ttttttatca tgctctattc cgcaagtaac aggctgttga
      361 gaataaaccg gacatagatt ccgaggcctg gcgtgaaacg cttatagcct tccaggttta
      421 ccttagtcca gttagccgta atacccggtt ccaggatgat gccgtttcca aattcaatgg
      481 aagcgcctcc cccggcaacc agtccgtaac ctatccctcc ttcgtataca ttgtatgctt
      541 gcatattgga attgggaata taggattgcc ttccataaac gttgatgatg gagtattctt
      601 tccccccgat gtacaggaaa tttttcaata ctttggtatt gttaatgttg atcccgccaa
      661 gggcaaaata cattacattt tttcgttggc tttcgaagta acgtttcagc gaaaggttga
      721 aataaaccct ttcttcaatt ccttttatcg gataaagcct tatatcggct tcggtggcat
      781 attctttcgg atcaacctct atgctgacgg catcgctggt tttgagtttc aggtaattaa
      841 actcggcatt cagggcgtaa tctttattga aacggtattc ggcaaacaat ccgggattca
      901 tagatacctt ataatgcatg tttgcgggaa ggccgttcgc agcaataaaa acagtatccg
      961 aagcatctag ggcattataa atttcctgat accagtagta attaccgaag acatagctgg
     1021 ctgtgttttc gttggaattg ctgccgttat aataattggc cgtatatttc ccgggataat
     1081 aaaggccgaa gttcagcccg aacgtccagc cgcagtattt atcttcttcc tggcaacgcg
     1141 cattccgcga agagaaaacc aacatgcaaa gaaggaggta tggtacaagc cgtttcatat
     1201 attttcagat cacgggaaca aaggtagtta ttttgagcct tgcagaaata ggggaggttt
     1261 cggcattaac taaatgttta acaggttttt aagatatcag gtcgataaaa agcatgacat
     1321 tgagataagt aatgactgat ccgatgagca gaagcattgc tgtataaaag gctgagttgg
     1381 cgtattttcc cattaccttt tgggaggatg tcaggtgtac ctgaaagaaa acagtgaaag
     1441 gcaattggat actgaggagc atctgtgaaa taacaagccc tttaaaggga tcgcctgtca
     1501 gaaaaattac caaaacggct atcacgaggg agagcaaggc gcccagccgg ctgtggttgt
     1561 cttttatatc aaaaggttcg ccgtaaaaac cggcaaaaat actccctccg gccatggctg
     1621 aggtgatggc tgaagcgatc ccggaaaata gcagggcaac ggcgaagatt aatgctgcat
     1681 gatttccaag caagggctga agcaggcctt gtgcctgttg cagctctgaa acccggatgt
     1741 ggtttccgaa aaaggccgat gcagccaaaa gtatcatggc gctgttgatg gcccagccga
     1801 taatcatgga gaataatgtg tcgaggaatt cgaaccgtaa ttgtttcctg atcactgttt
     1861 catcctgcag gttccattgc cggctctgga tgatttcgga atgcaggaaa aggttgtgtg
     1921 gcatcacgac ggcgcccagg acgctcataa ccacaatcat cgagcctttg ggtattgacg
     1981 gttttaccca gcctgtggcg gcttccatcc agttgatatt ggtcagtgaa agctcataaa
     2041 tgaaggaaag ccctattaca gaaacaaaac cgataatcca tttttcgagt cttcggtatg
     2101 aattggtaaa cagaaggatg caaatggtta tcattaagat gatagatcct gcttttacgg
     2161 ggatgccgac aagcatctga agggcgatgg ctccgccaag gatttcggcc atggatgtag
     2221 ccacagaagc tgccatggca gaggttagaa ggctccgcga gacaaaagtg cctgtaaatt
     2281 tcgttgcggc ttccgaaagg cacaagccgg ttgcaatgcc caggtgagcc acattgtgtt
     2341 gcaaaataac gagcatgatg gtcgatagtg taaccatcca aagcagttta tatccatagg
     2401 ctgatccggc ggccaggttt gaagcccagt tgccgggatc gataaaaccg acagtaacca
     2461 gcaaaccagg gccgatatat ttcagtattt caagcccgcc gaatacggga cggtaatcct
     2521 ttcggtcaat gtttttgatg gaattgtata aacgtgtcag catacccacc gggaagaagg
     2581 atgaaagtta caataaaaac agtacaacta tcattgcgga atattgtttg aaaccggggc
     2641 tgtttttacg gaatccgttt taaaatcctt ccggcggagg attggtgaaa ttccggtctg
     2701 atatcataaa ccacgcttca ggtagctgaa cagcaaattt aaatatgcca gggcagccct
     2761 gtaaatgccc ggactgctca tctcacgggg gcctgctatg ttaagaacca gtattccgct
     2821 gattttaatc cagccggtga cccggggaat gtcataatca gaagttaaat cgatggtcag
     2881 cagcggcttg agcttatgac ggcagatgtt ttcggtcagt cgggttccgc ggtcgaggat
     2941 tccggttgtg ataatcagtg tgccgtccga ttgttccaca ttcattcggg tacggacagg
     3001 atattctttg gaggaggttt ccgtaagcgg atagcgcaag gggatgattc cgtcatcggc
     3061 acgacggtgc agcgggcaat aacccccgca gggaattcct gctgccatcg ccgcatccag
     3121 ggccgcgcgg tcaacgccgg tttgtccgcc ggaaacaatc ctgagttggt tgggactatc
     3181 catcggtgga aataatagta ttcattgcct tgcaggagag gttgcagtgg tataaagata
     3241 taaagtcctg taaacaaaaa aaaagccggc aggttgccgg ctttttggaa aattaggttg
     3301 gtcaggttaa ctatattccg gaatgttccc gctttatttg tgaggtatcc ggaacaccgg
     3361 agccttttat ttcagtcccc tgcgttcgag caaaggttca acagagggct ctttgccacc
     3421 tgtaaactgt ttgtaaagtt tcatggcatc atcggtgccg ccgcgttcca ggatgttttt
     3481 ccggaagctg tcggctgtgg ctttattgaa aatgccgttc ttgctgaagt agctgaaggc
     3541 gtcggcatca agcacttcgg cccagatgta ggcatagtaa ccggcagagt atccgccgga
     3601 gaatatgtgt tggaagaagg tgctgttata gcggggcgga atggcatcga tcaggccgat
     3661 cttgtccatg gaatgttttt caaagcactg ggcatgaatt tctttgccgg caggcaccac
     3721 gtggtaatcc atgtccagga tggatgcggc cagatattcg gttgtggcaa aaccctggtt
     3781 gaatttatcg gcagcctgca tctttttaat aaggctctcg ggaattactt ctccggtttt
     3841 ataatgcctg gcatatactt tcagcacttc aggctctgaa gcccagtttt ccatgatctg
     3901 ggaaggaagt tccacgaaat ccctgggaac tgccgttcct gacagggaag cataggtgca
     3961 ttttgacagt aatccgtgca ggccatggcc gaactcatgg aagaaggtgg ttacatcgtc
     4021 ccagttgatg agagccggtt cgccggcaga aggagcggga aaattataat tcagcgaaac
     4081 aacaggacgg atatctttcc cgttttcgat gcgctggtca atataattgg tcatccatgc
     4141 tcctgaacgt ttttcaggac gggtgaaata atccaggtac agaagtccga tcagtttgcc
     4201 ggaggcatcc gttacttcat aagcatcgac atctttctgg tatacaggca catccggacg
     4261 ttttttgaaa ttcaggccat agagtttatg ggcggtgtta aaaacgcctt cgcgcacatt
     4321 cgaaacctca aaatagggtt tcagctcttc gtcgctgagg ttgtactttt cgttgcgtac
     4381 tttttcggaa tagtaccacc aatcccaggg ctgaactgtg aaggtgtttc cttccttgtt
     4441 tatcatagcc tgtatatccg actgttcgcg ttttgccatt tccagtgcag gcgtccatag
     4501 ctggttcagg agtttgtaaa cattttcagg ggtcttggcc atgttgtcgt cgagcacata
     4561 cgaagcataa tccggatatc ccatgatggc agctttttca ccccgtaagg caactgtttc
     4621 atcgataagg ggattgttgt catattttcc gccgttgcaa cggttcaggt aatattggta
     4681 caactggcgg cgcagggcgc ggttttcggc atattgaata aaagggatga cctccgtttt
     4741 attgagaccg aagatccatt ttccgtccag tcctttgttc cgggctgcgt cggcagcagc
     4801 gttgatgatg ttctgcggga ggcctttcag gtcgccggca ttatcgatgg tcatataata
     4861 accattggta gctttcagca cattgttgcc gaattcaata tccaggatgg caagtcgggc
     4921 attgatctcg cggaggcggg gctttttatc ttccgaaacc agggcgccat tgcggacgaa
     4981 attcttatag gtctcttgga gcagtttggc ctgttcgcct ttgagtttca gctttgaacg
     5041 gttatcataa acagccttca cacgggcaaa taatttctcg ttcaggtata tgttatcgta
     5101 atgctccgtt agtttcggcg atgattcccg gttgatttcc tgcagttcat cggaggtgtt
     5161 ggccgattcg aggttgctaa gcacagtacg gacacggtac aggatgttgc ctgatttttc
     5221 aagcgcctca atggtgttgg caaaagtggg aggctgtttc gagttgacga tcgcattgat
     5281 ctcgtcattg tgcattttta tgccctgctc aatggccggc atgtaatgtt catatttaat
     5341 gcggttgaag ggcggaactc cgaacggggt attccattgt ttttcgagga atggattttg
     5401 agccatagca gacaagccaa tgattagtgt ggctaaaaac agggtgtatt ttttcatgat
     5461 gagacggtga atgatttctg ccgtaaaggt atagaattta aaaaccccgc cgggatggaa
     5521 agacggggtt tttagggtta gtgccggttt tcttactgat tagcgcgtaa cggaaatcgg
     5581 tattattact gtaatggtgt tattacttcc agcacttcgg gcaggtccat acccaggttc
     5641 ttcatgtgtt cgatcgtagg gaccccgtta ctgttccagc cgcgacgttt gtacacggca
     5701 tcgagaagct tttcgtaacg gtcttcgcgg taagcgcgtg tggctttcat tttttcggcc
     5761 agcgtcatag tcgaaggatc gagattcatg aggtcgatga gttgtttgtc gtacctttcc
     5821 tggcggctga ggtattcctc ttcggttacc gggcctgccg cacggtaagg ctgggcgtcg
     5881 ttcttgcgca gtccgtatcc gcgccggata ttaaataccc gttgaaagtt gtaaaccctt
     5941 tcgctctggc ggatcaattc aactttatcg aaattggtat tcccggtaac agccttataa
     6001 atggtaacat agttatcgac atgttcggga acttttgccg gttcgcccga ctgcgcattg
     6061 ttctcgggct caacgtcgtt ccagggtagt ttacataatc cctggagtcc gaaccaggta
     6121 cggaacatgg gaaaataatg cagagcttcg gctttctttt caaaggttgg gatctggttg
     6181 ttgaccatat ccatgaatat gagccatgct tcgtcgtgtt gcgggccttt gttggtcatg
     6241 gcataaccac cctgctgtgc cagcgattcc tttgaaacat actgcgaata ctcgaggccc
     6301 ttgttttcca tgccgatatc ctgaaggaac tgcgcgtcgc cccagccgtt ttcagcaaag
     6361 atttgcttca ttttgtggac tcccaatccg gcgatcttgc caaatccttc accgcgcgaa
     6421 acccggtgaa gcagttccat ggcgctgtcg gcgttgccga aattcagttt cagcccgccg
     6481 gtgcgttcct cgttcagaat gccgttttca taacactcca tgatgaagcc catgatggtt
     6541 ccccaactga tagtgcagat gccgtatgtg tcgcaataga agttagcttc gatggtataa
     6601 tccgggttaa agatgccagc gattgatccc agtgaagaag ttgattcata ttcggggccg
     6661 tcgacgatta ccttcgagcc tttgtaaggc ccggtgcgaa gttcataatt atcaacgcct
     6721 tttgcgcagg ccatgttaca gccgatccag cagccgtcgg gcattccctg ggtaaagtac
     6781 gacttccata catcacccct gatattatgg gcatccgggt ggctcccgaa tttgaaatta
     6841 tgcgtgggca gcaggtcgta gtcgttcatg atatgggtaa ggtgggccgt tcctttggcg
     6901 cgcatttcgc attgcttgtc gtcgagctcg cgcatttcct tgttgaaacg ttttcccctt
     6961 tccatgatgg cttccagatc aacaacattg ttccggttac cggttacgcc tttgctgtgg
     7021 gcgacgacag cctttatttt cttattgcgg agtacggttc cgattccgcc ccggccggcc
     7081 tgtttcaacc ggaccagctt acgcctgaca tcgtagaaac tgaaattaag catcccgatc
     7141 agcgaattat cggcagcagt tccggccgaa actaccccga tatttacacg gtctttttcg
     7201 gttgaggcta atatttcggt aaggtattcg gcaagcagat ggctgtcggg ggattcattt
     7261 ccggcttcgt agatttcaac cacttcgcgg gtctcatcga tgagcacaat gacgtcctca
     7321 ccggctttgc cctggagttc cagcgaatca aaccctgcaa atttcagaaa aggcccgaaa
     7381 taacctccaa cgttgctgtc gatcacaata tcagtctgag gggaaatggt gacaaccagt
     7441 gatttaccac tgccggaata ttgcgtaatt ccgcagatgg gaccggtatt gatcacgatc
     7501 tcgttctcgg gatcatccca tttggtgccc ggcttggttt cttcccacaa caatttgagg
     7561 ccgaatcctt tgccgccggt aaatttttcg atcatcatct ggctgaccgg tttctcacgt
     7621 atagcgccgg ttccgacgtt tacgtgcagg gtgcgccggt tatatccctt ttcaatgggg
     7681 gcccagttgt atttgtacga atggagcaat ttgtgattgc tggtgaaatc ttctaatgcc
     7741 atatagttgg tttttccagg tacgattatt tcacaaaaat aagaaatctt tatattgcaa
     7801 ttgaccggtt cccttaatat tttacgatat gaaaagatct tcacatcgct tatctggcgc
     7861 cgctttccct gaaatggtaa tccgtggttc atgaggctgt tttaacatct tatttctgta
     7921 ctatatttgc gtggaattta aacagaaacc tgtggtccgg aacttatgac gtcggaagag
     7981 ataatgatga tcctgaaagg gaaaaaagca ggcatagccg gatgcggggg gctgggatcg
     8041 aattgtgccg ttgcattggc acgggcaggc gttggcgctc tggtaattgt tgattttgat
     8101 gtcatcgacg agagcaacct gaaccgtcag tattattttc gggatcaaat cggtatgctg
     8161 aaagtaaccg ccctgaagaa caatctggag aggatcaacc ctttcatcga tattagcgct
     8221 gtagaattgc ggttgtcgcc accggatatc cttaaatatt tctccggctg tgatgttatt
     8281 gtcgaagcct ttgatgatgc cggtatgaag ctgatgatga tagataccgt tttgaatcag
     8341 atgcctgaaa aatttttggt aacgggtgta gggatggccg gctggggaga taacaattcg
     8401 atcagaacgc gcatttcaga taagctggtg atttgcggcg acgaaaccaa tgaagtatcc
     8461 gccgatgtcc cggctattgc accccgtgtt ggggtggttg ccaacatgca ggccaatgcc
     8521 gttctcgaaa ttttgctggg gaaaaaccca ttccaggcaa tcaggaccag tttctgatcc
     8581 ggccatggag aaaaacttta ttgacctgat attgctgctg aaatcatgaa aataattctc
     8641 aataaccgtg aagaaatact cgatcagcat agcctgacga tcagcgagct gctgaaagtg
     8701 aagaactttt cctttaaaat gctggtggtg aaaatcaacg ggaacctcgt cagaaaaaat
     8761 gaatatgaca tcacgcgggt caacgatggc gacgatgtca tggtgttgca cctgatcagc
     8821 ggcgggtgag agggaaagct gaaatcgggg aacgggagcc ggaaaatgtt aatgccactt
     8881 tgcttaagga aggagttgcg cggaa
//
LOCUS       DAOPYV010000101         8636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_9717_length_8636_cov_13.070738, whole genome shotgun sequence.
ACCESSION   DAOPYV010000101 DAOPYV010000000
VERSION     DAOPYV010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9717_length_8636_cov_13.070738"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2531
                     /locus_tag="PKI35_10810"
     CDS             <1..2531
                     /locus_tag="PKI35_10810"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNX44917.1"
                     /translation="NVLEFKYIEVKNKAASLVLDISDQHLPNVYIEATLFKKITNTEM
                     PLTVAHGVISLKVDDPKARASVTITAPEKSRSNTRQVITVKTIPNAEVTVAVVDEGIL
                     LVTGYTSPDPYAWFYQKRALEVNSYDVYAQLFPELRRSGSSPAGGEAFDLSRRLNPLT
                     SKRVRLISKWSGILKSDGKGQVSFPVDIPQFSGALRVMAVAYKDEKFGSAEKTIRVAD
                     PVIVSSALPRFMSPGDKASMIVNVTNTTKAAAKATIEVSATAPLTVSGKTKQPATLGV
                     NHEQAYTFEVTAGNYTGNATVMVTVSALGEKFTEKIELPVRPASGLTFFTGSGIVKGG
                     TSKAFSVNSNLMPQGVRSRLMISKSPAAEYLKNLNDLIRYPYGCMEQTISAAFPQLYL
                     RDLATSAHRQLVAPSESDAGSPDENIQQAIYKVESHQLYNGGFPMWPSGGSDQWWISA
                     YATHFLLEAQKAGYQTNDLIFSNAVKYLEQKVKDRKTETYFYTDDAGAITSVSTPAQE
                     IFYSLYVLALADRAAIPTMNFYKSQASALTSENRYLLAAAYMLAGDRRSYQAVLPRTF
                     GTTKIRSLAGYSFASFIRDRAMALNALLEADPDNPQVGQLLLTIGKEMKQKRYLTTQE
                     NAFCMTAMGKFARKNAASNVKATVTINGKQTAPFTGSDLVLKDGLNGRKVNISATGNG
                     NLYYFYELSGIPVKPATGDEDHSIRVRKHFLDKNGNALSGNSFRPNDLIIVKITAETE
                     SGKTIENVAITDLLPACFEIENSRLVAERSYSWQQNVTTPDYQDIRDDRISFFATLGP
                     ATSTFYYTVRVVNKGTYTMGPVTADAMYNADFRSVSGSRVIKVE"
     gene            2736..4196
                     /locus_tag="PKI35_10815"
     CDS             2736..4196
                     /locus_tag="PKI35_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNX44918.1"
                     /translation="MSNNPIENLKELFRQWSGEEGLEVKALPQAGSYRQYFRINGHQR
                     QAIGVYSPDKQENEAFLSFTRHFRAAGLPVSEIYAEEASKNIYLLSDLGPESLFDLLE
                     RDPEENRPSESVRNLYRETIAWLPKFQVVAGAGIDYSLCYPVRDFDTRSMHWDLNYFK
                     YFFLRLLRIPFDEYALENDFDELTAYLLQTESRYFMYRDFQARNIMINNGRPYFIDYQ
                     GGRRGPLQYDLASLLFQVKANLPYDFRDEMLHWYLENVKRYTIVDEKDFIEKYHAFVL
                     IRLLQVMGAYGFRGYYERRAHFLQSIPYAVNNVRWLLENIRLEPKLPTLTGVLEQIAN
                     LNINSPFSASGQLLTVSINSFSFKNSPPPDYSGNGGGFVFDCRALPNPGRYEHFRQFT
                     GKDAPVIQFLEREPAIGKFLQTVYTLIDQSVKEYMERGFKNLQVNFGCTGGQHRSVYC
                     AEKLARYLEDRYSINVVLNHTQLKPSGEYRSPGSKD"
     gene            4359..4727
                     /locus_tag="PKI35_10820"
     CDS             4359..4727
                     /locus_tag="PKI35_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44919.1"
                     /translation="MLDKFDVETYKNQPEIIRQTLQQVQKDFGMFGFEVLFSGNAEAD
                     YKEMLVQLSDYVAVFMENDCHQFTALLYHIDIGEGKIKQALEQNSEQSMADVITELVM
                     HRELKKVLTRNYFKQEGMQV"
     gene            4724..5449
                     /locus_tag="PKI35_10825"
     CDS             4724..5449
                     /locus_tag="PKI35_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNX44920.1"
                     /translation="MKAMIFAAGLGTRLVPFTQTMPKAMVPVAGKPMIQWTIEKLIQS
                     GFDEIIINVHHFAGQIIEFVRKKNSFGIRIEFSDETDALLETGGGLLKASWFFDDNQP
                     FLVHNVDVVSDIDTAEMFGHHAENNAIATLAVSRRKTSRYFLFDTSLRLRGWKNINTG
                     EEKIPYPVKEALLPMAFGGIHVISPEIFPLITEKGRFSINDVYLRLSGHYPIRAYQHD
                     TEAWFDMGKPADLAAAGRYLEQL"
     gene            5473..8334
                     /locus_tag="PKI35_10830"
     CDS             5473..8334
                     /locus_tag="PKI35_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNX44921.1"
                     /translation="MKPFLQQLADELFDTYGEEVSKLCLVFPNRRAGLYFRRYLAGRI
                     SKPLWSPAIYSIEDFVALKSGLQIVGQPALLINLYTLYFSLLGEKARPLKDFITWGSR
                     LLSDFDEIDQYLVDGVSVFSYLTEAKAIEHWNPDGKALTDFEKSYLEFYNSIAPLYAQ
                     FRAELLNSNQAYMGLAFRRLIENLETTSFDEWQKIVFAGFNALTPVEEKLFTALINSG
                     KARIFWDADNYYVDDENHEAGLFLRKYRNRNLFGPMNWISGHYKEEEREINIAGIPKN
                     IGQAKFAGQLLSRLSGDETGKTAVVLVDEGLLIPVLNSIPAGITDFNVTMGFPLKLTP
                     AYSLADAIFTLLLNSEKYASGMNDQPVKRFYRQDILRLLKHPYLIQLSGGISNTGQGI
                     EGKLTCSFYPGNHLIQFLEKNCTPLCEVFGPYISNHVNKAQDTLALVQRAISLVKNSL
                     AEKNPRNPESDREFASIDMEYLFSIAGIIQNLYDLIALLPGDPGTETLHVLFTSIAGA
                     MRLPFYGEPLKGIQIMGVLETRTLDFDTLIMLSVNEGLLPRGKRQDSFIPMDIRLEYR
                     LPGFREHNAVFAYHFYRLLQRAKTTWLLYNTEADELGNGEKSRFITQLAYELPRYNPG
                     AKIPEQVIGIQSLKPADSKITISKNAGTMQRLREIAVTGFSPSVLSAYVTCSLQFYYS
                     AILGLKEPDSVDETIDASIIGNAIHHVLQKVYTPYVNRPVDSGIVRSLIGQAFGMVKP
                     ELERLFPGNDLETGKNLIIVNVAQNMIRRFLEAEAQYLSKIDGGTGSLRILYLEKKLT
                     GNLSISADGTGEIQYVMIKGTTDRIDAIGNQIRVIDYKTGSLMPFELNLDSPENLGMM
                     KKPQKMLQLLTYTWLYHVAHPGEKELVSGIISLKAPKKYLQKAKINKSDIIQEDAIRQ
                     FEKYLTGLLVEIFSQSAPFQQTDNPDNCKFCAFRSICNR"
     gene            8559..8632
                     /locus_tag="PKI35_10835"
     tRNA            8559..8632
                     /locus_tag="PKI35_10835"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8593..8595,aa:Asp,seq:gtc)
BASE COUNT         2356 a         2220 c         2022 g         2038 t
ORIGIN      
        1 acaacgtcct tgaattcaaa tatattgaag taaaaaacaa agcggccagc ctggtactgg
       61 atatctccga tcagcatctt cccaacgttt atatcgaggc caccctgttt aaaaaaatca
      121 ccaataccga aatgccgctc accgtggcgc acggggtgat ctcgctcaag gtcgacgatc
      181 ctaaagcaag ggcatccgta accatcaccg cccctgaaaa atcccgctca aatacccggc
      241 aggtaatcac ggtaaagacc attccaaacg ccgaagttac tgttgccgtg gttgacgaag
      301 gcatcctgct ggtaacaggc tatacttccc ccgatccgta tgcatggttt tatcagaaaa
      361 gggcgctgga agtcaattcg tacgatgttt atgcccaact gtttcccgaa ctccgtcgtt
      421 cagggtcaag cccggcggga ggcgaagcct ttgacctgtc gcgccggctt aatcccctga
      481 cttcgaaacg ggtaagactc atctcgaaat ggagcggcat tttaaaatcg gacggaaaag
      541 gacaagtctc tttcccggtc gacatcccgc agttttcagg cgcattacgc gtcatggccg
      601 ttgcttacaa ggatgaaaaa ttcggttcgg ccgagaagac aatccgggta gccgacccgg
      661 taattgtcag ctcagctttg ccccggttta tgagccccgg cgacaaagcc tctatgattg
      721 tgaatgtaac gaataccacc aaagctgccg ccaaagccac cattgaggtc tcggctacgg
      781 cgccgctaac ggtttcgggc aaaacgaaac aaccggcaac gctcggagta aaccacgaac
      841 aggcttatac tttcgaagta acggccggca actataccgg gaacgccacg gtcatggtga
      901 cggtaagtgc cctgggcgaa aaattcaccg aaaagatcga acttccggtc cggcccgctt
      961 caggacttac ctttttcacc ggttcgggaa tagtaaaagg aggtacttcc aaagctttca
     1021 gcgtcaattc gaatctgatg ccccagggtg tccggtcaag gttgatgatc agtaaatcgc
     1081 ccgcagccga gtacctgaaa aacctcaatg acctgatccg ttatccctac ggatgcatgg
     1141 aacaaaccat ttcggcagct tttccccagc tttaccttcg cgacctggca acatcggccc
     1201 accggcaact ggttgctcct tcggagagtg atgccggttc gccggatgaa aatatccagc
     1261 aggccattta caaagtagaa tcacaccagt tatacaacgg cgggttcccc atgtggcctt
     1321 caggaggttc cgatcaatgg tggatatcgg catatgcaac ccatttcctg ctcgaagcgc
     1381 aaaaagcagg atatcaaaca aatgacctta tcttttccaa tgccgtgaaa tatcttgaac
     1441 agaaagtgaa agaccggaaa acagaaacat acttttatac cgatgatgcc ggcgccatca
     1501 cctccgtctc aacaccggcc caggaaatat tctattcgct gtatgtgctg gcactggccg
     1561 acagggcagc catacctacc atgaatttct ataaatcgca ggcaagcgcc cttacttccg
     1621 aaaaccgcta tctgctggct gccgcatata tgcttgccgg cgaccgtcgc agctaccagg
     1681 ccgtcttgcc ccgtactttc ggcactacaa aaatccgatc cctggccggt tactcctttg
     1741 cctcttttat ccgcgaccgt gctatggccc tcaatgccct tctggaagcc gatcccgaca
     1801 atccccaggt agggcaattg cttctcacca ttgggaaaga aatgaaacag aaacgctatc
     1861 tcaccaccca ggaaaatgcc ttctgcatga cggcgatggg gaaatttgcc aggaaaaatg
     1921 ccgcttcgaa tgtaaaggct actgtcacca tcaatggaaa acaaaccgcc ccgttcaccg
     1981 gttccgacct cgtgctgaaa gacggcctga acggtcgtaa agtcaacatt tcggccacag
     2041 gcaacggaaa cctctattat ttctacgagc tatccggtat cccggtcaaa cctgcaaccg
     2101 gcgacgagga ccattccatc agggtgcgca agcacttcct cgacaagaac gggaatgccc
     2161 tgagcggcaa cagttttcgt cccaacgacc tgatcatcgt gaagattacc gccgaaacgg
     2221 aatccggcaa gacgattgaa aatgtcgcca tcaccgatct gcttccggct tgcttcgaaa
     2281 ttgaaaactc gcggttggtg gccgaacgca gttatagctg gcaacagaac gtcacaacgc
     2341 ccgattatca ggacatccgc gacgaccgga tcagtttctt tgcgaccctg ggccccgcca
     2401 cttcaacttt ctattatacg gtgagggttg tgaacaaagg cacttataca atggggccgg
     2461 taaccgccga tgccatgtac aacgctgatt tcagatcggt ttcagggagc agggtgatta
     2521 aagtggaatg atacgccggc agaccagtcc ccggacatac ccggatggtc cgtaacaatc
     2581 catgtataat tcgggcaatt cgtggtttta ttgttccctc tgagtcgcgt taccgggaaa
     2641 caatggatgg tgcagtcatc ccgtgaaata aaaaaatgct actttcgtac aatcactcac
     2701 aaaataaccc taataaagaa actttctcaa ttcctgtgtc aaacaatccc attgaaaacc
     2761 tgaaagaact attccgtcaa tggtcgggcg aggaaggtct cgaagtaaag gcacttcccc
     2821 aggcaggatc gtacaggcag tattttcgca tcaacggcca ccagcgacag gctatcggcg
     2881 tgtacagtcc cgacaagcag gaaaatgagg cgtttttgtc tttcacccgt catttccggg
     2941 ctgcgggatt gcccgtttcc gagatctatg cagaagaagc ctcaaaaaac atctaccttc
     3001 tcagcgacct gggcccggaa tcattgttcg acctgctcga acgggatccg gaggaaaacc
     3061 gtccttctga atccgtccgc aatctgtacc gtgaaaccat tgcctggctt ccgaaattcc
     3121 aggttgtggc cggcgccggt atcgattact ccctttgcta ccctgtccgg gatttcgata
     3181 cccggtctat gcactgggac ctgaattact tcaaatattt tttcctcagg ctgttgcgaa
     3241 ttcctttcga cgaatatgcc ctcgaaaacg acttcgatga attaacggca tacctgctgc
     3301 agacggaaag ccgttacttc atgtaccgtg atttccaggc caggaatatc atgatcaata
     3361 acggccggcc ttattttatc gattaccagg gagggcggcg cgggccgctt caatatgacc
     3421 tcgcttcgtt gctgttccag gtaaaagcaa acctgccata cgatttccgt gatgaaatgc
     3481 tccattggta tctcgaaaac gttaagcgtt ataccattgt cgacgaaaag gacttcatcg
     3541 aaaagtatca tgcattcgtg ctgataaggc ttttacaggt gatgggcgct tatggcttcc
     3601 gcggctatta cgaacggagg gcgcatttcc tgcaaagcat cccgtatgcc gtgaataatg
     3661 tccgctggtt gctcgaaaac attcgtctgg aacccaaact cccaacgctg acgggtgttc
     3721 tggaacagat cgcgaacctg aatatcaact cccctttttc agcttccgga cagctactta
     3781 ccgtcagcat caacagtttc tcgtttaaaa attcaccgcc ccctgattat tccggcaatg
     3841 gcggtggatt tgtttttgac tgccgcgcgc ttcccaaccc gggacgctat gagcatttcc
     3901 ggcaattcac cgggaaagat gcccccgtta ttcaatttct cgaacgtgaa ccggccatcg
     3961 ggaaattcct gcaaacggtg tatacattaa tagatcagtc ggtgaaagaa tatatggaaa
     4021 gaggttttaa aaacctgcag gtaaactttg gctgtaccgg cgggcagcat cgctcggttt
     4081 actgcgccga aaaactggcc cgttaccttg aagacagata ttctatcaat gtcgtattaa
     4141 accataccca actcaaacca tcgggagaat accggtcgcc cggcagcaaa gattgagctg
     4201 ttaccgaagc agcaagaaat atcacaagaa acgatctgaa aacaagaaat ccagaaaaga
     4261 aaacaaagaa acccggcccc ggaatcccgg atgcagggaa taaggattgc caggaagaaa
     4321 ggaaatgata taccaatgcc aacccgtgaa aatgaacaat gttggataaa tttgacgtcg
     4381 aaacatataa aaaccagccg gaaataatca ggcagacctt acagcaggtt cagaaagatt
     4441 ttgggatgtt cggcttcgaa gtcctttttt caggaaatgc cgaagcggat tataaggaga
     4501 tgttagtgca actgtcggat tatgtcgccg tatttatgga aaatgactgc catcagttta
     4561 cggccctgct ttaccacatc gacattggcg aaggaaaaat taaacaggcg ctggaacaga
     4621 attctgaaca gagtatggcc gacgttatca ctgaacttgt gatgcacagg gaactgaaaa
     4681 aggtgctgac gcgcaattat tttaaacagg aaggaatgca ggtatgaaag ccatgatctt
     4741 tgccgccgga cttggaaccc ggctcgttcc cttcacccaa accatgccca aggcgatggt
     4801 cccggttgcc gggaagccta tgatccaatg gaccatcgaa aagctcatac agtcaggttt
     4861 cgatgaaatt atcattaacg ttcatcattt tgccggacag atcatcgaat ttgtcaggaa
     4921 aaaaaactca ttcgggatac gcatcgaatt ttcggacgaa acagacgcac tccttgaaac
     4981 cggcggcggt ttattaaagg cttcctggtt cttcgacgac aaccagcctt tccttgtcca
     5041 taacgtggat gtggtttccg acatcgacac ggcggaaatg ttcgggcatc atgctgaaaa
     5101 taatgcaata gcaacgcttg ccgtaagccg tcgcaaaacc tcaaggtatt tcctgttcga
     5161 tacgtcactc cgtcttaggg gatggaaaaa cattaacacc ggcgaggaaa agatacctta
     5221 tccggtaaag gaagccttac tgcccatggc ttttggcgga attcatgtaa tcagcccgga
     5281 aatattcccg ttgatcaccg aaaagggccg gttctccatc aatgacgttt accttcggtt
     5341 gagcggccat taccctatcc gggcatatca gcacgacacg gaagcctggt tcgatatggg
     5401 caagcctgcc gatcttgcag ccgccgggag ataccttgaa caactttaac aatttacact
     5461 aaaaatcacc tgttgaaacc attcctgcaa caactcgccg acgaactttt cgatacatac
     5521 ggagaagagg taagcaagct ttgccttgtt ttcccaaacc gcagggcagg tttgtatttc
     5581 aggcgctacc tggccggccg tatcagcaag ccattgtggt caccggccat ttattccatc
     5641 gaagattttg tcgcgctcaa atcgggtctc caaatcgtcg gccagcctgc cctgctcatc
     5701 aatctgtaca ctctgtactt ttcgctactt ggcgaaaaag ccaggccgct gaaagatttc
     5761 atcacctggg ggagccgtct gctttccgat tttgacgaaa tcgaccagta cctcgtcgac
     5821 ggcgtatctg ttttcagcta cctcaccgaa gccaaagcca ttgagcactg gaatccggac
     5881 ggcaaagccc tcactgactt tgaaaaaagc tatctggaat tctataattc cattgctccg
     5941 ctatacgccc aattcagggc tgagctttta aacagcaacc aggcatacat gggacttgcc
     6001 tttcgcaggc tcatagaaaa ccttgagacc acctccttcg acgagtggca aaagatcgtc
     6061 tttgccggct tcaacgccct gacgcctgtt gaagaaaaac ttttcacggc tttgatcaat
     6121 tccggaaaag caaggatatt ctgggacgcc gataattatt acgttgatga tgagaatcat
     6181 gaagcaggcc tttttctgag aaaataccgc aaccggaacc tgtttggccc catgaactgg
     6241 atttcaggac attataagga ggaagaacgg gaaatcaaca ttgccggcat cccgaaaaat
     6301 attggccagg ctaaatttgc cggacaactg ctcagcagac tcagcgggga cgagacagga
     6361 aaaacagccg ttgtactggt tgatgaaggc ttgcttattc ccgtattaaa ttccattccg
     6421 gccggcatca ccgatttcaa cgttacaatg gggttccccc tcaaactcac accggcatac
     6481 tcgctggcgg atgccatttt caccctgctt ctaaactctg aaaaatatgc atccggaatg
     6541 aatgaccagc cggtgaagcg cttctatcgc caagacatac tccgtttact gaaacaccct
     6601 tacctcattc aactgagcgg gggaatatca aatacggggc aaggcataga aggaaaactc
     6661 acctgttcgt tttatcccgg gaaccacctg attcagtttc tggaaaagaa ctgtacccct
     6721 ctgtgcgaag tattcgggcc ttatatttcc aatcatgtca acaaggcgca ggatacgctc
     6781 gctttggtgc aacgggccat cagcctggtt aagaattcgt tggccgaaaa aaatccgcgg
     6841 aacccggaat ctgacaggga gttcgcctcc atcgacatgg aatatctttt tagcatcgcc
     6901 ggcattattc agaacctgta cgatttgatt gcccttttgc caggcgatcc gggaactgaa
     6961 acgcttcatg tcttatttac aagtatcgcc ggggccatgc gccttccttt ttatggtgaa
     7021 ccgctcaaag gaatccagat aatgggagtc ctggaaacca gaacgctcga tttcgatacg
     7081 ctgattatgt tatcggtaaa cgaaggatta ctccccagag gcaaacggca ggattcattc
     7141 attccaatgg atatcaggct tgaatacagg ctgccgggat tccgcgaaca taatgccgtt
     7201 tttgcttacc atttttacag gcttttgcag agggcaaaaa cgacatggct tctttacaat
     7261 accgaagccg acgagttggg aaacggtgaa aaaagccggt tcatcactca gttggcatat
     7321 gaactgccac gatataatcc cggcgcgaaa attcccgagc aggtaatcgg aatccaatcc
     7381 ctaaagcctg ctgacagcaa aatcacgata tcaaaaaatg caggcacgat gcaacggctt
     7441 cgtgaaatag ccgtaacggg cttctcccct tccgttttga gcgcctatgt tacgtgcagc
     7501 ctccagtttt attattcggc catattggga ctgaaagagc ctgactccgt tgatgaaacc
     7561 attgatgcca gtatcatcgg gaatgccatt caccatgttt tgcagaaagt ctatacccct
     7621 tacgtcaacc gtccggttga ttccggcata gtaagatcgc tcatcggaca agctttcggg
     7681 atggtcaagc cggaattgga acggctcttc ccgggaaacg atctggagac aggcaaaaac
     7741 ctgatcatcg tcaatgtggc tcagaacatg atcaggcgtt ttctggaagc cgaggcgcaa
     7801 tacctgtcga aaatagatgg cggaaccgga tcgctcagga ttctgtacct ggagaaaaaa
     7861 ctaactggta acctttctat atcggccgac ggaacaggag aaatccagta tgtcatgatt
     7921 aaaggcacta ccgacaggat cgacgcgatt ggaaatcaaa tacgggtcat cgattataaa
     7981 acgggatcgt taatgccttt tgaattgaac ctcgacagcc ccgaaaacct gggaatgatg
     8041 aagaagccac aaaaaatgct gcagttgctg acctacacct ggctatatca tgttgcgcat
     8101 cccggtgaaa aggagctggt ttcgggtatt atttcgctga aagcgcccaa aaaatatctc
     8161 cagaaggcaa aaattaacaa gtcggacatc attcaggagg atgccatccg gcaatttgaa
     8221 aaatacctca ctggcctgtt agtggaaata ttcagccagt cggcgccttt tcaacaaact
     8281 gataacccgg acaattgcaa gttttgtgca ttccgttcga tttgcaaccg gtaaccggcg
     8341 acaggggatc atgtctgtca ggaatttact tttaaaaaaa attttaccgg ttgtttgaat
     8401 attcggggaa agtgttacct ttgcgccctg tttaagaaca aagttcattg aatcggcccg
     8461 gttgttctgt tggttagaat tagtgcctgt catcttgtca cgagtgacga gacggggtcg
     8521 agtcaaggaa aaatgcagat aaagttctta taaatattgg ttcggtagtt cagttggtta
     8581 gaatacgtgc ctgtcacgca cggggtcgcg ggttcgagtc ccgtccggac cgcttc
//
LOCUS       DAOPYV010000102         8465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_10004_length_8465_cov_12.232224, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000102 DAOPYV010000000
VERSION     DAOPYV010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8465
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10004_length_8465_cov_12.232224"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(377..958)
                     /locus_tag="PKI35_10840"
     CDS             complement(377..958)
                     /locus_tag="PKI35_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNX44922.1"
                     /translation="MIKSLKISSGWSLFLDRDGVINRRIPDDYVKGPSGFEFLPGVTE
                     ALSIFSEVFRYIVVVTNQQGIGKGLMSVRDLESVHQKMVGEIENCGGRIDKIYYCPDL
                     KDSRSFSRKPAVGMGLKARKDLAGLKLHQSVMAGDTASDMLFGKRLGMTTVLISDDVN
                     EIRKCSEMADFSFPDLISFARRLKNEGREEGLG"
     gene            complement(955..1674)
                     /locus_tag="PKI35_10845"
     CDS             complement(955..1674)
                     /locus_tag="PKI35_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNX44923.1"
                     /translation="MRQSKPDVIVLAGGLGTRLQDAVPGVPKCMATLAGKPFLEYLLN
                     YLQNNRLQRVILSLGYLADVVKEHFGDRYGTLALEYSIEDEPLGTGGAVKRALPLAAT
                     ESVFVLNGDTFFDVDLPEMLHQHNLSKALVTIALHEAQNAGRYGVVAINSDGRITSFS
                     EKQPGVGRGLINGGIYLVEKDYLSEHPLTGTFSLEKDLFQQECHSTMIYGYHSNGYFI
                     DIGIPDDYRRACEEIPELMRL"
     gene            complement(1688..2272)
                     /locus_tag="PKI35_10850"
     CDS             complement(1688..2272)
                     /locus_tag="PKI35_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HNX44924.1"
                     /translation="MKQLITDTIRQSVSVKNEILNDPVLIETIEKVAGEMVTCFRNGG
                     KVLFCGNGGSAADAQHLAAELSGRYYYDRDPLPAEALHVNTSYLTAVANDYSFDEIYA
                     RMVKGMGRKGDMLIGFSTSGNSANVVKAFKQALEKGMITAAFTGKTGGKLQESSCYLL
                     NVPSTDTPRIQESHIMIGHIICQLVESALFPRGK"
     gene            complement(2298..3323)
                     /locus_tag="PKI35_10855"
     CDS             complement(2298..3323)
                     /locus_tag="PKI35_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HNX44925.1"
                     /translation="MSIIRSKAPLRISLAGGGSDVSPFSDMYGGAILNATLNLYATAS
                     IEPAADGKIRLDLPDRGVSLELDSSGEIPVHGEAILHAGVYNRIIRDFVHKPLSFKLI
                     TNVDAPPGSGLGTSSTLAVAIIGAFAEWLKLPLGDYDIAHLAYEIERIDLGLAGGKQD
                     QYSATFGGVNYMEFSENDKVVVNPLRIKPIYLDELANNLVLYYTATSRASAGIIEAQQ
                     RNVVNGNKTSIEAMHRMKEQALMMKEALLRGNLDEIGEILNYGWHYKKMMADEISNPF
                     IDSVYDTALKNGATGGKISGAGGGGFMLFYCPGNTRYAVIESLKAFGGETRKFDFTKS
                     GLCTWTI"
     gene            complement(3324..4478)
                     /locus_tag="PKI35_10860"
     CDS             complement(3324..4478)
                     /locus_tag="PKI35_10860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX44926.1"
                     /translation="MDTPRNVIILGTAYPFRGGGISTFNERMARAFQEKGDRVVIYTF
                     SLQYPSILFPGKSQYSDEAPPENLEIEVAVNSVNPFNWIKVGRKLRHLNPDLVIIRYW
                     IPFMAPCLGTIASIIRKNRHSKVVAITDNIIPHEKMPGGKMLSKYFVNRCDAFVSMSR
                     SVLGDLQQLDRKKPKSYTPHPLYDNFGEPVAKEVAKQRLGLSPDCGYILFFGFIREYK
                     GLDLLLKAFADSRLRQLPVKLLIAGEFYVDPKPYLDLIANLGISGHVELRNGFIPNNE
                     IVNYFCASDVVAQPYREATQSGITQVAYHFNKPMITTNVGGLSETVPDGKVGFVVNPD
                     SDEIAEALLKYFEGGKEKEFSENIAEEKKRFSWDAFVGIIDGLLNRIKSN"
     gene            complement(4512..5450)
                     /locus_tag="PKI35_10865"
     CDS             complement(4512..5450)
                     /locus_tag="PKI35_10865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX44927.1"
                     /translation="MDISIVVPLFNEEESLPELCQWIERVMKENNFSYEVLLVDDGST
                     DNSWTVVEELHAANSNLKGIKFRRNYGKSAALNQGFQAASGKVVITMDADLQDSPDEI
                     PSLYDMITKDGFDLVSGWKKKRYDSKLAKNLPSKLYNATTRRMSGIKLHDMNCGLKAY
                     RLEVVKSIEVYGEMHRYIPVIAKWAGFRKIGEKVVEHRKRKFGVTKFGWERFINGFLD
                     LLTIMFVSKFGKKPMHLFGSLGTILFLIGFVIAGYLAWARLFFSAYRMTDRPIFYFGL
                     LAMILGTMLFLTGFLAELVSRSSPDRNHYLVEKEIK"
     gene            complement(5455..6027)
                     /locus_tag="PKI35_10870"
     CDS             complement(5455..6027)
                     /locus_tag="PKI35_10870"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HNX44928.1"
                     /translation="MKDKKALFNASVIASLYIAGAGILYSLLQYVLGILPVGMFKPFL
                     IGGLGLVISLVILVILLKKYRKTQGGFISFGDAFLFGLIALVLAAVISSVYSYLFMKF
                     FDPEYMKNILEAQKDWMENYFSSKGMSEEQIQAYLDKADQQANLPILKQTLKSFLISS
                     VFSAVIALIVGAIMKKNRDVFNDQKTGGVI"
     gene            6134..6233
                     /gene="ffs"
                     /locus_tag="PKI35_10875"
     ncRNA           6134..6233
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKI35_10875"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            6389..7045
                     /gene="fsa"
                     /locus_tag="PKI35_10880"
     CDS             6389..7045
                     /gene="fsa"
                     /locus_tag="PKI35_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNX44929.1"
                     /translation="MKFFIDTANLDQIREAQALGILDGVTTNPSLMAKEGIKGKDAIL
                     KHYIDICNIVDGPVSAEVISTDFDGMITEAEILSSLHENIVVKIPMIRDGIKAIRYLS
                     DKGIRTNCTLVFSAGQAILAAKAGATYVSPFIGRIDDSSVDGLDLIDQIVTIYRIHNF
                     ETRVLAASIRNTLHIVKCAELGADVVTSPLASILGLLYHPLTDIGLAKFIEDSKKMGL
                     "
     gene            7082..7696
                     /locus_tag="PKI35_10885"
     CDS             7082..7696
                     /locus_tag="PKI35_10885"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNX44930.1"
                     /translation="MLIRLYPENPSERQVRMIVECLRDGGIIIYPTDTIYGIGCDIFK
                     VKSVERIAELRGLKPGKTMFSFICHDLSQLSEYTKPIDNHLFKVMKANLPGPFTFILN
                     ANNNVPRHIQSKRKTVGIRIPDNQIPLDIVRELGNPIMSTSVHDDDEILEYTTDPELI
                     YEKYRNLVDIVIDGGYGGNIPSTVVDCTEDDIVVTREGKGELVY"
BASE COUNT         2056 a         2172 c         1920 g         2317 t
ORIGIN      
        1 gccttgcgca aggctgtggg agggggcagg gtttcagagt tctgcgtttt gtgttttgtg
       61 tccctcgttc tccgttcttc gttctccgcg atccctattc ctttgaccct gccatctcac
      121 ccaaagccct gcgcaaggca ggtaaacttt gaccttagac ttttgacttt tgacttttgc
      181 ttttagccat tagcctgcca actcacacac tatctaaccc aggtaaactt ttagctttta
      241 cgttgtgcgt attgtgtttt gcgttttgag gtttccgttt ccccttcttc attctccgtt
      301 ctccgttctc cgttctcctt gctccctgct cccagccata tgacttttac ctttccctcc
      361 gccagtcatg ctaaaatcag cctaagccct cttccctgcc ttcatttttc aacctgcggg
      421 caaagctaat caaatcgggg aaagaaaaat cggccatttc cgaacattta cgtatctcat
      481 tgacgtcgtc cgaaataaga acggtcgtca tacccaaccg cttgccaaac aacatatccg
      541 aagccgtatc gccggccatt acactctgat gcaactttaa tccggcaagg tctttcctcg
      601 cctttagccc catccccaca gccggttttc gtgaaaaact ccgggaatcc ttcaggtcgg
      661 gacagtagta tatcttatca attcgtccgc cgcaattttc aatttccccg accattttct
      721 gatgaaccga ctcaaggtct cttacggaca tgagaccttt cccgattccc tgctggttgg
      781 tgacaaccac gatatacctg aagacttccg aaaagattga taaagcttcg gttacgcccg
      841 gcaaaaattc aaaaccggat ggtcctttca cataatcatc aggaatacgg cggtttatca
      901 cgccatccct gtcaagaaac aacgaccatc cggaggatat tttcagggat tttatcataa
      961 gcgcatcagt tcaggaattt cttcacaggc gcgacggtag tcgtcaggaa taccgatatc
     1021 aataaaatat ccgttgctgt gatatccgta gatcatggtt gaatggcatt cctgctgaaa
     1081 aaggtctttt tcgagtgaga atgttccggt cagcggatgc tccgataaat aatccttttc
     1141 aaccaggtag ataccgccgt tgatcagtcc ccgcccgacg ccgggctgct tttcactaaa
     1201 ggaggtgatc cgtccgtcgg agtttattgc caccacccca tacctcccgg cattctgggc
     1261 ctcatgcagc gcgatggtca ccagcgcttt cgacaggtta tgctgatgca gcatttccgg
     1321 aaggtcaaca tcaaaaaaag tatcgccgtt cagcacaaaa acgctttcgg tagcagcaag
     1381 aggaagcgcc cttttcacgg cgccgccagt tccaagcggt tcatcttcaa tggaatattc
     1441 aagggcgaga gttccgtaac gatccccgaa atgctctttt acaacatcgg ccagatatcc
     1501 cagcgagagg atcacccgtt gcagacgatt gttctgcaga taattcagca ggtattccag
     1561 aaaaggtttt ccggctaagg ttgccataca tttgggcact cccggaaccg cgtcctgcaa
     1621 acgcgtacca agtcctccgg cgagaactat gacatctggt tttgactgtc gcatgatatt
     1681 tcctgaatta ttttcccctg gggaataagg ccgattcgac aagctggcag atgatatgtc
     1741 cgatcatgat gtgcgactcc tggatacgcg gggtatcggt tgacggcaca tttaaaaggt
     1801 agcaactgct ttcctgaagc tttcctccgg tttttccggt gaacgctgcc gttatcattc
     1861 ccttttcaag ggcctgtttg aaagctttga cgacgttggc cgaattaccg gaagtggaaa
     1921 acccgatcag catgtcgcct ttgcgcccca tccctttgac catacgcgca taaatttcat
     1981 cgaaggaata gtcgttggca acggccgtga gataagaggt attcacatgc agggcttcgg
     2041 ccggtaaagg gtcacggtcg taataatacc ttcccgaaag ttcggctgcc agatgctggg
     2101 cgtcggcggc gcttccgccg ttgccgcaaa aaagtacctt accgccgttc ctgaaacagg
     2161 ttaccatttc accggcaacc ttttcgatgg tttcgatcag gacaggatcg ttgaggatct
     2221 cattcttgac gctgaccgac tgcctgatgg tatctgtaat taattgtttc atttctgttc
     2281 aagtattttt cggatgatta aatcgtccag gtgcaaagcc cggatttggt aaaatcgaat
     2341 ttcctggttt ccccgccaaa cgccttcagg ctttcaatca ctgcgtaccg cgtatttccc
     2401 ggacagtaga acaacataaa tccgccgccg ccggcgcccg aaatcttgcc tccggtagcg
     2461 ccgtttttta aagccgtatc ataaacactg tcgatgaacg ggttggagat ttcatcggcc
     2521 atcatcttct tgtaatgcca gccgtaattc aggatttcgc ctatttcgtc gaggttgccg
     2581 cggagcaagg cttctttcat cataagggcc tgctctttca tgcggtgcat agcctcgatg
     2641 gaagtcttgt tgccattcac gacattccgc tgctgcgctt cgatgatccc tgccgaagcc
     2701 cggctggtgg ccgtgtaata cagtaccagg ttgttcgcaa gctcatccag gtatatcggc
     2761 ttgatccgca acggattcac aaccacttta tcgttttccg aaaactccat gtagttcacc
     2821 ccgccgaagg ttgccgaata ctgatcctgc ttcccgccgg ccagtcccag gtcgatgcgc
     2881 tcaatttcat aagccaggtg agcaatatca taatcgccca gcggcaattt aagccattcg
     2941 gcgaatgccc cgatgatggc taccgccagg gtcgaggatg ttcccaatcc cgagcccgga
     3001 ggcgcgtcga cgtttgtgat gagcttaaac gacagcggtt tatgtacaaa atcgcgaata
     3061 atacggttat ataccccggc atgcagtatg gcctctccgt ggaccgggat ttctcctgaa
     3121 gaatccaact ccaggctgac acccctgtcg ggcagatcca gccgtatttt tccgtcagca
     3181 gccggttcaa tgctggcggt ggcatacagg ttcagcgtcg cgttgaggat agcgcctcca
     3241 tacatatcgc tgaaagggga aacatcggac cccccgcctg ccaggctgat gcgcaacggc
     3301 gctttgctgc gtataatgga catttaattc gactttatcc ggttgagtaa accatcaata
     3361 atgccaacaa aagcatccca tgaaaaacgc tttttctctt cggcaatatt ttcagaaaat
     3421 tctttttcct tcccgccctc aaaatatttc aagagggctt ccgctatttc gtcgctgtcg
     3481 ggatttacaa caaatcccac tttaccatca ggaaccgttt ccgaaagccc tccgacattg
     3541 gtggtgatca tgggtttatt gaaatgatag gcaacctgcg taatcccgct ttgcgtcgct
     3601 tccctgtaag gctgggcaac tacatcgctg gcgcagaaat aattaacgat ttcgttattg
     3661 ggaataaaac cattgcgcaa ctctacatgt ccggagatgc caagattcgc gatcaggtcg
     3721 aggtatggct tcggatcaac ataaaattct cctgcgatga gcaatttcac aggtaactgc
     3781 ctcaaccggc tgtcggcaaa tgctttcagc aaaaggtcca gtcccttgta ttccctgata
     3841 aatccgaaga acaggatata tccgcaatcg ggcgacagcc ccagacgttg ttttgcgacc
     3901 tctttggcca ccggttctcc gaaattatca tacaacgggt gcggcgtata gcttttgggc
     3961 tttttacgat caagctgctg aaggtcgccc agcaccgacc gcgacatcga aacaaaggcg
     4021 tcgcaacggt tcacaaaata cttcgacagc attttgcccc ccggcatttt ttcgtggggg
     4081 atgatgttgt cggtgatggc aaccaccttt gaatgcctgt ttttccggat gatggaggca
     4141 atggttccca ggcacggagc catgaaggga atccagtacc ggatgatcac caggtcggga
     4201 ttgaggtgac ggagttttcg tccgactttt atccagttga aaggattcac cgaattgacc
     4261 gcgacctcta tttcgaggtt ctcgggcggc gcttcgtcgg aatactgtga tttccccggg
     4321 aaaaggatgg acggatactg aaggctgaaa gtatagatca caacgcgatc gcccttttcc
     4381 tggaacgccc gggccattct ttcgttaaag gtgcttatgc cgccgcccct gaaaggatag
     4441 gctgtgccaa ggatgatgac atttcgtggt gtatccattg taaagcggtt atccggtttt
     4501 ccggaaccgg tttatttaat ttctttttca acgagataat ggttgcggtc gggcgaactt
     4561 cttgaaacaa gttcggccag aaaaccggtc aggaaaagca tcgtgcccag gatcatggcc
     4621 agcagtccga aataaaagat aggcctgtcg gtcatacggt aagctgagaa aaacagccgc
     4681 gcccaggcaa ggtaacccgc aattacgaac cctatcagga acagtatcgt accaagcgac
     4741 ccgaaaaggt gcatgggctt tttcccgaat ttcgaaacga acatgatcgt cagcaggtca
     4801 aggaaaccgt tgataaaccg ttcccatccg aatttagtca ccccgaattt gcgcttgcgg
     4861 tgttccacca ccttctcccc tatcttccgg aaaccggccc atttggcgat tacgggaata
     4921 tagcggtgca tttcgccata tacttcaatg cttttgacaa cctctaggcg gtaggctttc
     4981 aacccgcagt tcatatcatg cagcttaatc cccgacatcc ggcgggttgt ggcattgtac
     5041 aatttcgacg gcaggttttt ggccagtttc gaatcgtagc gtttcttttt ccagccggag
     5101 accaggtcga agccatcctt tgtaatcatg tcgtaaagcg acgggatttc gtcggggcta
     5161 tcctgcagat cagcatccat agtaatgacc actttgcccg atgctgcctg aaatccctgg
     5221 ttgagtgcag cggatttgcc gtaattgcgc ctgaatttaa tgcccttgag gtttgaattt
     5281 gcggcatgca gttcctcgac aacggtccat gaattatccg tactgccgtc atccacgagc
     5341 agcacctcat aggaaaaatt attctccttc atcacccgtt ctatccactg acagagttcg
     5401 ggcaatgatt cttcttcgtt aaaaagaggt actacgattg aaatatccat catatcagat
     5461 tactccgcct gttttctgat cgttaaacac atccctgttt tttttcataa tggccccaac
     5521 tatgagagca atcacggcag aaaataccga actgatgaga aacgacttaa gggtttgttt
     5581 tagtatcggc aaatttgcct gttgatcagc tttatccaga tatgcttgta tttgttcttc
     5641 actcatacct ttggatgaga agtaattttc catccaatct ttctgggctt ccaggatatt
     5701 tttcatgtac tccggatcaa agaatttcat gaaaaggtag gaatatacgg aagatatgac
     5761 tgcggctaaa accaatgcaa tcaggccgaa taaaaaggca tctccaaagg aaataaaacc
     5821 tccctgtgtt ttcctgtact ttttcaataa aatgacaaga ataaccaggc ttatcacaag
     5881 cccgagccct ccgatcagga atggcttgaa catgcctacc ggcagaatac ccagtacata
     5941 ttgcagaaga gaatacagga ttccggcgcc ggcaatatat aaagaggcta ttacagaagc
     6001 attgaataaa gcttttttat ctttcatggc cgtttatttt taggttagat gcaaaggtat
     6061 aaaagatggc gggaaatact aaaagcgtat tgctaattgt aatttggttc cgaaaaatgt
     6121 gtacttttgc aacgggtaag tcttatacga ccagctcctg ttgaactccc ccaggtcagg
     6181 aatgaagcaa gggtagacgg ttgtagcggt gcgatataag tagcttaccc ttcttttttt
     6241 atatcattta atattggtta agatggtatc cggatacatg atttgatcgt aaatttgccg
     6301 caggttttca ggaaaaacat cccgaaaccg gttcttcccg aacctgaaga ttcagcatct
     6361 aaaattgtcc caccaaataa agtccctcat gaaatttttt atagacacag ccaatctcga
     6421 tcaaatccgt gaagcccagg cccttggcat cctcgacgga gttactacca atcccagcct
     6481 gatggcgaaa gaaggtatca aaggcaaaga cgccatcctg aagcactata tcgacatctg
     6541 taatattgtg gatggccccg tgagcgccga agtaatttca accgactttg acggaatgat
     6601 taccgaagcc gaaattctct cttcgctcca tgagaatatc gtggtaaaaa ttcccatgat
     6661 cagggacggc attaaagcca tccgatacct ttcggacaaa ggcatccgca caaactgcac
     6721 actggtattt tcagccggac aggctatcct ggcagcaaaa gccggcgcca cctacgtttc
     6781 gccgttcatc gggaggattg acgactccag cgttgacggt ctcgacctta tcgatcagat
     6841 cgttacgatc tatagaattc ataattttga aacccgggtc cttgccgctt ccatccgcaa
     6901 taccctccac attgtgaaat gtgccgaact gggcgccgat gtagtcacca gcccgctggc
     6961 ctccatcctg gggcttttat accatccgct caccgatatc ggcctggcaa aattcatcga
     7021 agactcaaaa aaaatgggcc tctgattttt cccaatccca accaccagga actccttgac
     7081 tatgctaata cgcctctatc ccgaaaatcc aagtgaacgg caggtccgca tgattgtgga
     7141 atgcctccgc gacggaggga tcatcatcta tccgaccgat acgatttacg gaattggctg
     7201 cgacatcttc aaggtaaaat cggtggagcg tatcgccgaa ttaagggggc tgaaacccgg
     7261 gaagacgatg ttttcgttta tctgccatga tctcagccag ttgtcggaat acaccaaacc
     7321 gatcgacaat catctcttca aggtcatgaa agcaaacctg ccgggaccgt ttacatttat
     7381 cctgaacgcc aacaacaacg taccgcggca tattcaaagc aaacgtaaaa cggtaggcat
     7441 ccgtatcccc gataaccaga tccccctgga tattgttcgg gaactgggta atcccatcat
     7501 gtcgacttcg gtgcatgatg atgatgagat ccttgaatat accactgatc ccgaactcat
     7561 ttatgaaaaa taccgcaacc tggttgatat cgtgatcgac ggcggttatg gcggtaatat
     7621 tccttccaca gtggttgact gtaccgagga cgacatcgtg gttacgcgtg aaggaaaagg
     7681 ggaattggtt tactgacatc tgttgtatgg cgatccgttc tcggggcgcg taatatcccg
     7741 aagcttggtt tacgtttctt ttggctgtta gcctgccgtc ttacccacac ccgccccagg
     7801 caggtaaact tttagccttt agcgttttga gttttgcgct ttgagttttg agttctgtgt
     7861 tttgcgtttc gtggtattcg tgattgcatt cgtgaaattc gtggctcgtg attcgttgtt
     7921 cgttctgtgt tttgcgtttc gtggaattcg tgatagcatt cgtgaaattc gtggctcgtg
     7981 attcgttgtt cgttctgtgt tttgtgtttt gcgtttcgta atcagtaatt cgtggaattc
     8041 gtgattgcat tcgtggaatt cgtggtttat gatttgacct ttgacctttg acctttggct
     8101 tttggctttt ggcttttggc ttttggctgt tagctctttg ctcctcgctc cgtgttccgt
     8161 tctccatgct ccgtcctccg ttccccgtcc tccgtcctcc gttctccgtc ctcccctgcc
     8221 tacctctcac acaaagccaa acaccggcag gtaaagttct tcgttctcct ttcccggatt
     8281 ccgatctccg tataacgctg tttctttctt taagaaattg gttatcaatg gttacagaac
     8341 ataatgaaaa gtttttcccg atatctgttt gttaaaccag gaaaaaacgt atttttgcac
     8401 cctcaaaact aaaggatgat tcgctagctc agcaggtaga gcacatccct tttaaggatg
     8461 gggtc
//
LOCUS       DAOPYV010000103         8166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_10520_length_8166_cov_12.036494, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000103 DAOPYV010000000
VERSION     DAOPYV010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10520_length_8166_cov_12.036494"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1124
                     /locus_tag="PKI35_10890"
     CDS             111..1124
                     /locus_tag="PKI35_10890"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNX44931.1"
                     /translation="MKNFDENLLLRKIQGITTPEEEQQVEKWLAVSEENRKNYAYIKM
                     LWNARKVEEYSSGPDLERSLSEINRRIDRLHSGQPVLGIRKMLKYAAVFLGIVGLSVL
                     IWQFRHATSEEPMISKVAPVKGAVEMVLLPDGTKVWLNHGARLTWPETFQKKSRCVKL
                     EGEAYFEVTKDPSRHFFVKTPLLTTEVLGTSFNINTKAGGNTTQAVLVEGSIALLSPS
                     GKRIAKLEPGHMASASSLSSGIEITEVNTDVYTAWQQGKVVFEKAGLTEILDKLEEVY
                     NVRFRYDPDLMRKNKARYNFVFRINQDFDTVFSMLKFVAPLETLKVKPINANNTTNSK
                     SSR"
     gene            1269..4646
                     /locus_tag="PKI35_10895"
     CDS             1269..4646
                     /locus_tag="PKI35_10895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX44932.1"
                     /translation="MKEKAKAQAFDRSFLIKTLIMSNMIVALVLCSVLTAFAGETYAQ
                     KVRLNLEYKNVPLQTVMDDIKKQTEFEFAYESDIEDIIVKKVSVHAKNELIDDVMQKV
                     LKGTGIKFRIIDKIILLSNKEMKTTSGNTEMQPQKIVVSGMVKDKTGPLPGVSINIKN
                     TYEGTTSDQNGSFTLSVPDLNATLVFSYIGYLTQEIPLAGRSKLDVLMVQDVMNLDEV
                     VVVGYGTQRKKDLTGAVSTVNGNDLQKLPVANVEQALQGQVAGVQVTQNYGNPGAGVQ
                     IRIRGIGTIGDSDPLYVIDGVPTKEGINSLNTIDIESISILKDASAAAIYGARAANGV
                     VLITTKKGQTGVTQVQFNTQYGVQQISKKLDLLTADQYTMVMDEALKNDGLEPVWNNP
                     GLGAGTDWQDVIFRKAAFRKYDLSVTSGTENTSYLLGIGYYGQDGIVKYSDFTRYNVR
                     FNLNSNISKKFTVGTNMNISRISEDLIDTEINGVVRSAIFQPPTISVYNEDGSYAGPG
                     ANEGDAQNPLGMADRSDKTSLNNKLFGSLFATYQIIPSLKFKSNLGLNVYSLQTKDFD
                     PTFSEGNANRQVNSLTTRDVEYFDVTWENTLDYTKEFRDHTFNILAGSTLQDAQTNTL
                     TGYRESFINNEGYLQYLDAGSANDKARGNMTEWSLLSYFGRINYDYAGKYLLSVNTRF
                     DGSSRFGKSNRWGVFPSASAGWRISKESFFNVPFIDDLKVRASWGQLGNQDIGLYAFT
                     STLQQIYYTFGNPQSIYVGYYPASDFNPDVKWETTTQLDFGFDMYAFDYRLYFSADYY
                     KKNTSDMLLILPQPATSGFGSTGYENLGEVENKGWEFQALWRDKIGKVGYNISANVTT
                     SKNKVLELGEYSDAITTGLFFDMSTRTEVGHSIREFYGYVTDGIFQNQDEVDAANAKA
                     AELNGEGTVYQNLLTAPGDIRFKDLNKDGKINSDDRTFIGNPYPDLFIGLNMGLNYRN
                     FDFSMFFQGQFGNDIYNATDFWLTNSGYNYNKGTAILDRWTGEGTSNSEPRLTTLDPN
                     QNARGSDRYIEDGSYVRLKNLQLGYTLPESASQKIWLKGLRVFVTATNLLTFTDYTGY
                     DPEVGAARATLGDRTVGFDEVTYPQNKSFIVGLNVTL"
     gene            4671..6137
                     /locus_tag="PKI35_10900"
     CDS             4671..6137
                     /locus_tag="PKI35_10900"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX44933.1"
                     /translation="MKRLIHIIPPLALILILAAASCTKDDFLEKNPNGVLSTGNFYKT
                     AGDAEAAINGAYSILLDFWAESIAFKKDIISDDAVKGQGVDLAALTNFDNLNFSATDG
                     VNQTIWGLYFKGVYLTNLVLDYVPAIDMDAAQKDRILGEAHFLRAFYYYTLTIRYGGL
                     PLISSTINAEYYPKRATAADTWAFIETDLRKAAELLPLKSAYGSSDIGRASKGAALSL
                     LGTAYLFQEKWQDAFDTYGQVIQSGEYSLEPEFGNLFKPEYDNGTEVVFETQFKGGGE
                     FSYGNGFNFWVRPRNGSTIFGLGFCMPTQSLVDEFEAGDPRLKYTVIRPGDIISDEVS
                     GFPFQAEWSPETGMSCGKYVVGVTVGSNAERHEQNLKQIRYAEVLLGYAEAAYRLGNT
                     GEAVDKINMIRARARGSNTEVLPALDGSEDIFAAIVHERRVELALEGKRYFDLVRWGL
                     AEEKLSAKGYQPARKGLYPIPQTERDVNPNLVQNPGFE"
     assembly_gap    6173..6272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6302..>8166
                     /locus_tag="PKI35_10905"
     CDS             6302..>8166
                     /locus_tag="PKI35_10905"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HNX44934.1"
                     /translation="MPSEIPFRVNSIVLNNVYDKESELGVSKILTGINFANLAMTIDG
                     ETVDSASVSHWRQTLNMKEAWLETSFLFRDKAEITYRIYALRNMPYAAVMDIDVKPLK
                     GRIMMTASGIISAPPAYTVKNATFRILKDNEIAMPLLQTTAMSPFGRHLIAGTASFLF
                     NGGESPVLEHHEISNTEHRLSFQREIAPGERYNFAWAGAVCTTQDFSDPQNESERMVI
                     CLLRDNKEKAIKEHKKLWAELWQGDIIVEGNPADQQDIRLALYHLYAFSRPDAGLSIP
                     PMGLSSTIYNGHIFWDTELWMFPPLLAFHPEIAASLLNYRYNRLDKARSKARNYGYKG
                     AMFPWESDDTGEEATPSWALTGTFEHHITADVGIAFWNYYRVTGDKEWLKETGYPVMK
                     EIADFWVSRAEKNPDGSWSVNNVVGADEFAPNVDDNAFTNGSAKAILRYAVQAASVLG
                     IASPAEWKEVSGNLRFNYMKNGVMMEHSRYNGEIIKQADVNLLAYPLEIVTDPDSILR
                     DLRYYEPRIAAEGPAMGYSILSVLSSRMGDADRAYELFRKSFYPNKRQPFGALAEAAQ
                     FNNTYFATGAGGMLQAVLFGFAGLHITDHGIIQKEPCLPRQWKSLTLTGVGKDKAT"
BASE COUNT         2220 a         2024 c         2012 g         1810 t
ORIGIN      
        1 ccgtggctcc gtggtaaata atccgaagcg attagaaaaa atctcatttt tttgaaaaag
       61 ttctaagtag tttttccggt tgagttgtca ataaggaaaa caaagaggct ttgaaaaact
      121 tcgatgagaa tttattattg agaaaaatcc agggtataac cactcctgag gaagaacagc
      181 aagtggaaaa atggcttgcc gtatcggaag aaaacaggaa aaactatgct tatataaaga
      241 tgctctggaa tgcccggaaa gtggaagaat actcttcagg ccctgatctt gagcgctccc
      301 tctcggaaat caaccggaga attgacaggc ttcattccgg acaaccggtg cttggcatcc
      361 ggaagatgct gaaatatgca gccgttttcc tgggcattgt cgggttgtcg gtcctgatat
      421 ggcaattccg gcatgctact tccgaggagc ccatgatcag caaagtcgcc ccggtaaaag
      481 gagccgtaga aatggtactg cttccggacg gaacaaaagt ctggctgaat cacggggcca
      541 ggcttacctg gcccgaaacc tttcagaaaa agagccgctg tgtaaaattg gaaggggaag
      601 cctattttga ggttaccaaa gatccgtcca ggcacttctt cgtcaaaacg cctctgctga
      661 ccaccgaagt gctaggcaca tcgttcaata tcaatacaaa ggccggagga aataccacac
      721 aggccgtgtt ggtggaaggt tccattgcat tgctcagccc gtccgggaaa aggatcgcaa
      781 aactcgaacc cgggcatatg gcgtctgcat cgtcgctttc gtcgggtatt gagattaccg
      841 aagtcaacac cgacgtatac accgcctggc agcaaggcaa ggtagtgttt gaaaaggccg
      901 ggttaacgga aatactggat aagcttgaag aagtttataa tgtccggttc cggtacgatc
      961 ccgacctcat gcgtaaaaat aaggccaggt acaattttgt tttccgcata aaccaggact
     1021 tcgatacggt gttttcgatg ctgaaattcg ttgcgcccct cgaaaccctc aaagtaaaac
     1081 ctataaacgc taacaatact accaacagca aatcaagccg ttaaccgcct gaaccaataa
     1141 aatttaaacc agctaaccat gggaccctga agtaaaaaaa agccgggaaa aaggtgagag
     1201 tttttcccgg ttaaaatgat tcaactaaaa aaaccacaaa gtcttgttaa ttaaaccttt
     1261 acaaaattat gaaagaaaaa gcaaaagcgc aggcttttga caggagtttt ttaatcaaaa
     1321 cgctcattat gtcaaacatg atagtggctt tggtgctttg ttcggtttta acggcgtttg
     1381 ccggggaaac atatgcacag aaagtcaggt taaacctcga gtacaaaaac gtacctctcc
     1441 agacggtgat ggatgatatc aaaaagcaga cggaatttga gtttgcctat gaatcggaca
     1501 tcgaagatat catcgtgaag aaggtatcgg tacatgccaa aaacgagctg atcgatgatg
     1561 tcatgcagaa ggtgctaaaa gggacgggga taaaattcag gatcattgac aaaatcatcc
     1621 tcctttcgaa caaagaaatg aaaacaacct ccggcaatac ggagatgcag ccgcagaaaa
     1681 tagtagtaag cggtatggtg aaggacaaaa ccggcccgtt gcccggagtt tccatcaata
     1741 ttaaaaacac ctatgaaggc actaccagcg accaaaacgg cagtttcacc ctttccgttc
     1801 ccgatctgaa tgccacgctg gtgttctcct atatcggtta tctcacgcag gaaatcccct
     1861 tagcgggacg ttcaaaactg gatgtgctga tggtacagga tgtcatgaac cttgacgagg
     1921 tggttgtagt aggttatggc acgcaacgta aaaaagacct gacaggcgca gtatcgacgg
     1981 taaacggcaa cgaccttcag aaattgccgg ttgcaaacgt ggaacaggcg ttgcagggac
     2041 aggttgcagg cgttcaggta acccagaatt acggtaaccc gggcgcaggg gtgcagatcc
     2101 gcatcagggg gatcggcacg atcggcgaca gcgacccgct ttacgtgata gacggagtgc
     2161 ccacaaagga aggcatcaac agcctgaaca ccatcgacat cgagtcgatc agcatactca
     2221 aggacgcctc tgccgccgcc atttacggtg caagggctgc taacggcgtc gtgctgatca
     2281 ccaccaaaaa gggacaaacc ggcgtcaccc aggttcaatt caacacccag tatggcgtcc
     2341 agcagatctc gaaaaagctc gatcttctca ccgccgacca gtataccatg gttatggacg
     2401 aagcgctgaa aaacgacggg ctggaacctg tttggaacaa tccgggttta ggcgccggca
     2461 ccgactggca ggatgtcatc ttcaggaaag cagctttccg caaatatgac ctttcggtaa
     2521 ccagcggcac cgaaaacact tcctacctcc tgggaatcgg ctattacggg caggacggca
     2581 ttgtcaaata ttcggatttt accagataca acgtccggtt caacctgaac tccaacattt
     2641 cgaagaagtt taccgtaggc accaatatga acatctccag gatttcggaa gacctgattg
     2701 atacggaaat aaacggcgtt gtaaggtcgg cgatcttcca gcctcccacc atttcggtgt
     2761 acaacgagga cggaagttat gcaggccccg gggcaaacga aggcgatgct cagaatccgc
     2821 ttggcatggc cgacaggtcc gacaaaacat ccctgaacaa caaattgttc gggagcctgt
     2881 ttgcaaccta tcagatcatt ccttccctca aattcaaatc aaaccttgga ctgaacgttt
     2941 actctttaca aaccaaagac ttcgatccca cattttcgga agggaatgcc aaccgtcagg
     3001 tcaacagcct taccacccgc gacgtggaat attttgatgt tacctgggaa aataccctgg
     3061 attacaccaa agagttcagg gatcacactt tcaacatcct ggccggcagc accctgcagg
     3121 acgcccagac caacaccctt acgggataca gggaaagctt catcaataat gaaggatacc
     3181 ttcagtacct ggatgccggt tcggcaaacg acaaagcaag gggcaacatg accgaatggt
     3241 cgctgttatc ctattttggc cgcattaatt atgattatgc cggcaaatac ctgttatcgg
     3301 tcaacacccg cttcgacggt tcttccaggt tcggcaagag caaccgctgg ggcgttttcc
     3361 cttcggcatc agccggatgg agaatctcca aggaatcgtt tttcaatgta ccatttatcg
     3421 acgatctgaa agtccgcgct tcgtgggggc aactcggcaa ccaggatatc gggctgtacg
     3481 cgtttaccag cacccttcag cagatatatt acaccttcgg caatccgcag tcgatttatg
     3541 tcggatatta tccggcttcc gattttaacc cggatgttaa atgggaaacc accacgcagc
     3601 ttgactttgg tttcgacatg tatgcttttg attacagact gtatttttcg gcagattatt
     3661 acaagaaaaa cacctcggat atgcttctga tccttcctca gccggcaaca tcgggtttcg
     3721 gcagcaccgg ttatgaaaat ctcggcgaag tggaaaacaa aggctgggaa ttccaggcgt
     3781 tatggcggga taaaatcggc aaggtaggct acaacatcag cgccaacgtg acgacttcga
     3841 agaataaggt gctggaactg ggagaataca gcgatgccat caccacagga ctgttttttg
     3901 acatgtcgac gcgcacggaa gtaggccatt ccatccgtga attttatggt tatgtaacgg
     3961 acggtatttt ccagaaccag gacgaggttg acgctgctaa tgccaaggca gcagagctca
     4021 acggagaagg aaccgtgtat cagaaccttt taacagcgcc cggcgatatc cggttcaagg
     4081 acctgaacaa ggacggcaag ataaacagcg acgaccgtac cttcatcggt aacccctatc
     4141 ccgacctttt tatcggcctg aacatgggcc tcaattacag gaatttcgat ttttcgatgt
     4201 tcttccaggg gcaattcggg aacgatatct acaatgctac cgacttttgg ctgaccaatt
     4261 cgggatataa ctacaacaag ggcactgcca ttttagacag gtggaccggt gaaggcacca
     4321 gcaacagcga gccacgtctc acgacgctcg atcccaacca gaatgcccgc ggctccgaca
     4381 ggtatattga agatggttcc tatgtacgtc tgaaaaacct tcagttgggt tatacactgc
     4441 ccgaatccgc atcacagaaa atctggctga aaggcctcag ggtattcgta accgcaacca
     4501 atctgttaac attcaccgat tatacgggtt acgatcctga ggtaggtgca gcccgtgcca
     4561 ccctcggcga ccgcaccgtc ggtttcgacg aagtcacgta tccgcagaac aagagtttca
     4621 tcgtcggact gaatgtcaca ttgtaacaaa acatttaaat tcctgtgaaa atgaaaagac
     4681 ttattcatat aattcccccc cttgccctga ttcttatcct ggccgcagcc tcctgcacca
     4741 aggatgactt tctcgaaaag aatcccaacg gcgtgctttc gaccggcaat ttctataaaa
     4801 ccgccggcga tgcagaagcc gccattaacg gcgcctattc gatactgctc gatttctggg
     4861 ccgaaagcat cgctttcaaa aaggatatca tctccgacga tgccgttaaa gggcaaggtg
     4921 ttgacctggc ggcgcttacc aatttcgaca acctgaattt cagcgccacc gacggggtga
     4981 accagactat ctggggcctc tatttcaaag gggtgtacct caccaacctc gtgctcgact
     5041 atgtgcctgc cattgacatg gatgccgccc agaaagaccg catcctgggc gaagcacatt
     5101 tcctgagggc gttttactat tataccctca ccatccgcta tggcggtctt ccgttgattt
     5161 caagcaccat caacgccgaa tactacccta aaagggctac cgccgccgat acctgggcat
     5221 ttatcgaaac cgacctcagg aaggctgcgg aacttctgcc cctgaaatcg gcatacggtt
     5281 cttccgatat tggccgggct tcaaaaggcg ctgcactctc attgctggga acggcatacc
     5341 tgttccagga aaaatggcag gatgcctttg acacttacgg ccaggtaatt caaagcggcg
     5401 aatacagcct ggagcctgaa ttcggcaacc tctttaaacc ggaatatgac aacggcacag
     5461 aagttgtctt tgaaacacag tttaaaggag gcggggaatt ctcttacggc aacggattta
     5521 atttctgggt aaggccgaga aacggatcca ccattttcgg gcttggcttc tgcatgccaa
     5581 cccagagcct tgtcgacgag tttgaagccg gagacccgcg cttaaaatac accgtcattc
     5641 gccccggcga catcatttcc gatgaagtca gcggctttcc attccaggcc gaatggtctc
     5701 ccgaaacggg gatgagctgc ggcaagtacg tcgtcggcgt aaccgttggc agcaatgctg
     5761 aaaggcatga acagaacctg aaacagatcc ggtatgccga agtgctcctg ggatatgccg
     5821 aagcagctta ccggcttgga aatacaggcg aagctgttga caaaatcaat atgatcaggg
     5881 cccgcgcccg tggcagtaat acagaagtcc ttccggctct cgacggctcc gaagatatct
     5941 ttgccgctat cgttcacgaa cgtcgcgttg aacttgccct ggaagggaaa cgctattttg
     6001 atctggtcag gtggggcctt gccgaagaaa agctgagcgc caagggctat cagcctgcac
     6061 gcaagggatt ataccctata cctcagaccg aacgggatgt aaatccaaac cttgttcaaa
     6121 atcctggttt cgaataacct ggttagtttg ttgattggtt gagggtgtct cannnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntagcgctg tcgggcggga gattagggct
     6301 catgccttcc gaaatccctt tcagggtaaa ttccatcgta ctgaacaacg tttatgataa
     6361 ggagtcggaa ttgggagtga gtaaaatcct caccgggatc aactttgcga acctggccat
     6421 gacgattgac ggcgagaccg tcgacagcgc ctctgtttca cactggaggc agaccctgaa
     6481 catgaaagaa gcctggctcg aaacatcatt cctgttcagg gataaggccg aaataacgta
     6541 tcgcatctat gccttgcgga acatgcccta tgcggcggtc atggacattg acgtcaaacc
     6601 gctgaagggc aggatcatga tgacagcctc cgggatcatc agcgccccac cggcatatac
     6661 ggtgaagaat gccactttca gaatcctgaa agataatgag attgccatgc cgctgttgca
     6721 aacaacggct atgagccctt tcggacggca tctgattgcc ggaacggcat catttttatt
     6781 caacggcggc gaatcgccgg tcctggaaca tcatgaaata tcaaatacgg agcaccggct
     6841 atcctttcag cgggagatag cccccggtga gcggtacaat tttgcatggg ccggagccgt
     6901 atgtaccacg caggatttca gcgatccgca gaacgaatcg gaacggatgg tgatctgtct
     6961 tttgcgcgac aataaggaga aagccattaa agaacataaa aaactttggg cggaattatg
     7021 gcagggggat atcatcgttg aaggcaatcc ggctgaccag caggatatcc ggttagcctt
     7081 gtatcacctt tatgcttttt ccagacccga cgccggcctg agcattccgc cgatggggct
     7141 atcttcaacc atttataacg gacatatatt ctgggataca gaattgtgga tgttccctcc
     7201 cctgctggca tttcaccctg aaatcgcggc gtcgctgctc aactaccgct acaacaggct
     7261 tgacaaggcg cgcagcaaag cacggaatta cgggtataaa ggggccatgt tcccctggga
     7321 atcggatgat accggcgaag aagccactcc ttcgtgggcg ctgacgggaa cctttgagca
     7381 ccacatcacg gccgatgtag gcatcgcttt ctggaattat tacagggtta caggcgacaa
     7441 ggaatggctg aaagaaaccg ggtatccggt tatgaaagag atcgccgatt tctgggtcag
     7501 cagggccgaa aaaaatcccg acggttcgtg gtcggttaac aatgttgtag gggctgatga
     7561 atttgcgccc aatgtagacg ataatgcttt caccaacgga tcggccaaag ccatactgcg
     7621 ttatgccgta caggcggctt ccgtgctggg aatcgcttct cctgccgaat ggaaagaggt
     7681 ttcgggaaac ctccgtttca actatatgaa aaacggcgtc atgatggagc attcgcgtta
     7741 taacggagag atcatcaagc aggccgatgt aaacctgctg gcttacccgc tggaaattgt
     7801 gaccgatccg gacagcatac tcagggatct ccggtattat gaaccgcgca ttgccgccga
     7861 agggccggcc atgggctatt ccatactttc ggtgcttagt tcaagaatgg gtgatgccga
     7921 tcgcgcctat gaattgttca ggaaaagttt ctatcccaac aagcggcagc ctttcggcgc
     7981 acttgccgaa gctgcgcaat tcaacaacac ctatttcgcc acgggcgccg gaggaatgct
     8041 tcaggcggta ttgttcggct tcgccggatt acatatcacc gatcatggaa tcatacagaa
     8101 agaaccctgc cttcccaggc agtggaaaag tctgaccctc accggcgtcg gaaaagataa
     8161 ggcaac
//
LOCUS       DAOPYV010000104         8044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_10732_length_8044_cov_12.919890, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000104 DAOPYV010000000
VERSION     DAOPYV010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8044
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10732_length_8044_cov_12.919890"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..902)
                     /locus_tag="PKI35_10910"
     CDS             complement(138..902)
                     /locus_tag="PKI35_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX44935.1"
                     /translation="MSELALITGATSGIGEASARKLASQGYNLIITGRRRERLIKLAG
                     EVTSQFGVKVQWLCFDIRKRKEVEQAMASLSPEWQAVDVLINNAGLAAGLNLLQEGSV
                     DDWEQMIDTNIKGLLYITRFVAPKMIERGKGHIINLGSIAGKEVYPKGNVYCATKFAV
                     NALTKAMRVDMLQHGIKVSQVCPGAVETEFSEVRLKGDKQAAANVYKGFKPLTAGDVA
                     EAIWWIISQPAHVNINDLVIMPVSQAAPPYHHKKTE"
     gene            complement(1224..1931)
                     /gene="deoD"
                     /locus_tag="PKI35_10915"
     CDS             complement(1224..1931)
                     /gene="deoD"
                     /locus_tag="PKI35_10915"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF004489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNX44936.1"
                     /translation="MSIHIEAKTGEIADFVLLPGDPLRARHIAENLLEDAVCYNNIRG
                     MLGYTGFYRGRKVSVQGTGMGLPSLTIYVTELINDYGVKSLIRVGTCGGIMESIKVRD
                     LLIAISASTDSSIVARQFEGMTFAPTASFNLLKKAVEMAGLKGIPYKAGNILSSDLFY
                     HDPLKSDAYEIWRRYGTLAVEMESAGLYMLASRYGVDALSLLTVSDHILTGEHVPPLE
                     RQENFTKMSELALSLAI"
     gene            complement(2047..5733)
                     /gene="purL"
                     /locus_tag="PKI35_10920"
                     /gene_synonym="purI"
     CDS             complement(2047..5733)
                     /gene="purL"
                     /locus_tag="PKI35_10920"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNX44937.1"
                     /translation="MVQFFRSGKTAYAVQYSGGSQPDDISKLEWLFSEARLVDQETLA
                     GFFVGPRREMITPWSTNAVEITQNMGISGIARIEEFTEVAGEKAPYDHMLQRMYHGLD
                     QEIFTIHKEPEPVMYIGDIAEYNRKEGLALSEQEVGYLDEVSRKLGRKLTDSEVFGFS
                     QVNSEHCRHKIFNGKFVIDGEEKPESLFQLIKKTSKAHPGRIVSAYKDNVAFIDGPPV
                     TQFAPATQDKPDVFEVKDIDSVISLKAETHNFPTTVEPFNGAATGSGGEIRDRMAGGK
                     GSLPIAGTAVYMTSYPRTAEEHEWEKATEPREWLYQTPEEILIKASNGASDFGNKFGQ
                     PLICGSVLTFEHFENLKKSGYDKVIMLAGGIGSARKEDAMKGHPQPGDKLVVMGGDNY
                     RIGMGGGAVSSVATGEYHSAIELNAVQRSNPEMQKRVYNAIRALAESDANPIISVHDH
                     GAGGHLNSLSELVEEQGGQIDITKLPVGDPTLSAKEIIGNESQERMGLVIKEKDAALL
                     RRIADRERAPMYEVGEITGDMQFGIVNPQTGEKPLDIRLEYLFGKPPVTIMHDVRKQE
                     AYRDVAYDKENIYQYVSRVLQLEAVACKDWLTNKADRSVTGRIARQQCAGPLQLPLND
                     VGVVSLDYTGTKGIATSIGHAPVSGMIDAAAGSVLSVAEALTNLVWAPIEQGLDGVSL
                     SANWMWPCRNEGEDARLYSAVKAISDFAIALGINIPTGKDSLSMTQKYPGGEVVLAPG
                     TVIISAAGEVTDVRKVISPVLKEQLTSSLVYIDFSKSAFELGGSSFAQAVNRIGKTTP
                     TVDDAAYFRKAFAAVQELIGKEMILAGHDVSAGGLITTLLEMTFSRNTLGLGIDLSAL
                     GEKDIVRLLFSEKPAVVIQTNDLDFVSIFLHERGIEFSVIGKPVAGREVKIVNSGEEW
                     VFGIDALRDDWFRTSYLLDRKQSGEDFALERFRHYATAERKFRFNSSFTGTCEQYGID
                     PDRRTRTGIPAAIIREKGSNGDREMAWAMHLAGFDVKDVHMTDLITGRETLEDVNFIV
                     FVGGFANSDVLGSAKGWAGAFLYNPKAKEALDRFYAREDTLSLGVCNGCQLMAELGLI
                     YPGHEKKHKLLHNAGKYESSFLTIDILENNSVMLKSLAGTRLGIWVAHGEGRFMFPYE
                     ESRYNIPAKYSYSTLPGNPNGSMFDAACVASDDGRHLAIMPHPERAMHPWQWAYYPED
                     RRNDEVSPWIEALVNAREWIWERM"
     gene            6018..7772
                     /locus_tag="PKI35_10925"
     CDS             6018..7772
                     /locus_tag="PKI35_10925"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX44938.1"
                     /translation="MNFSEFNLNPQLLRAAAELGFETPMPVQQEVIPLFLGTDRDLVG
                     LAQTGTGKTAAFGLPILQLISPETKSAQALILAPTRELCMQIAGDIRDYAKHMPNIRI
                     TPIYGGAGFQGQLKELAAGPQVIVATPGRMLDMIKRGNIRLGNIRFLVLDEADEMLNM
                     GFEEDVSAIISNTPATRRTFLFSATMPAQVEQIAMKYMKSPETVTVGQRGKGASTVKH
                     LYLIAHASDRYKVLKRIADYYPNIYGIVFCRTRQETQDIADSLIRDGYSAEALHGDLS
                     QSQRDYVMNKFRIGHLRMLVATDVAARGIDVEELTHVINYNLPDELEVYVHRSGRTGR
                     AGRAGISISIINLKEKHKIRQLEKEVGKEFSQVRIPTGHQICEKQLYHVIEKMENVEV
                     NEEEVEEFLPVIYKKLNWMTQEELIKRFVSVEFNRFLDYYRDAGDLNVDESRDSQGST
                     KFARVFMSIGRKDGLIPPRLIGVINDFTGIRDMKIGKIEIGDTFSFVEIESRYTDQFM
                     QAFKGKFYNNRPVRVDEAEGQHKKRRTGEKPRKETKSGPDNNTAFSNKDRRKKWKTES
                     DPYFGDKNPKEGRRKKRF"
BASE COUNT         1984 a         2141 c         1977 g         1942 t
ORIGIN      
        1 aacgttatcc atagtatagc cgataattat tctcttgtga agagcgtaaa gataatgcaa
       61 aaatttaaat gcaaatccgc tcttttattc agcggttttt actgacggat gaccggatca
      121 ggctgcttga taaacaacta ttccgttttt ttatggtgat acgggggtgc ggcttgtgat
      181 accggcatga tgaccaggtc gttgatattg acatgagccg gctggctgat gatccaccag
      241 atagcttcgg caacatcgcc ggctgtcagc ggtttgaatc ctttataaac gttggcggca
      301 gcctgtttgt cgcctttgag ccggacttcc gagaattcag tttcaacagc cccgggacat
      361 acctgcgaaa ccttgatgcc atgctggagc atatccacgc gcatggcttt ggtaagggca
      421 ttgaccgcaa atttggtcgc gcagtaaaca ttgcctttgg gatacacttc cttaccggcg
      481 atggatccga gattgataat atgccccttg ccccgctcaa tcatttttgg cgcaacaaag
      541 cgcgtgatgt acagcaagcc tttgatgttg gtatcgatca tctgctccca gtcatcaaca
      601 gagccttcct gcaacaggtt caggccggcg gcaagaccag cgttatttat cagcacatca
      661 acggcctgcc actcaggcga cagggatgcc atagcctgtt ccacttcttt ccgtttccgg
      721 atatcaaaac aaagccattg aacttttacc ccgaattgtg aggttacttc accggcaagt
      781 tttattaacc tttcccggcg gcgcccggta atgatcaggt tatacccctg tgaggcaagt
      841 ttacgggcgg aagcctcacc gataccgctg gtggcgccgg tgatcagagc taattcagac
      901 atagtaatca aactttattg aacattaaga acacgctgtc aggcttttac tgaaaagccc
      961 ggccggagtt gccgctttgc agatattgca aaggattcac aggcaaaact acccattttt
     1021 caccatcggg tcatctgcta aatttcaaca atacctgttc atttccattc cgtggatgaa
     1081 acattgcctg ccatgtcatg atcacggaca atcctcaggt ccctcacgga ctgtttcgat
     1141 ccggggtaac gcctgcctgt atccacctcc tcaaaaggag gtgaataaag ttaattaaag
     1201 aagtctgcga gaattccgga gcctcagata gccagagaaa gggccagctc cgacattttt
     1261 gtaaagtttt cctggcgttc gaggggcgga acatgttcgc cggtgagaat atgatcgctc
     1321 acggtcagca gcgacaaagc atccacgccg taacgcgatg ccagcatata taatcctgcc
     1381 gattccatct ccacggcaag cgttccatac cggcgccaga tttcataagc atcggatttc
     1441 agcgggtcgt gataaaacaa atcgctcgat agaatatttc cggctttgta aggaattcct
     1501 tttaaaccgg ccatctccac agccttcttt aataagttga aggatgctgt tggagcgaaa
     1561 gtcatgccct caaactgcct ggcaacgatc gatgaatcgg tggaagcgct tatggcaatc
     1621 agcaggtcgc gtacttttat gctttccatg attccgccgc aagtacctac cctgattagt
     1681 gatttcacgc cgtaatcatt gatcagttcg gtaacataga tggtaagcga aggaagcccc
     1741 atgcccgtgc cctgcaccga cactttccgg ccacggtaaa aacccgtata gccaagcatc
     1801 cccctgatgt tgttgtaaca aacggcatcc tccagcagat tttcggcgat atgccttgca
     1861 cgcaaaggat cgccgggcaa cagcacaaag tcagcgattt cgccggtttt ggcttcaata
     1921 tgtatgctca tgagtgttca gataaaggat acaaatataa atagattcct tgattttgtg
     1981 ccggatttta cacttcccta aatcccggaa atgttcaaaa aggcgggctt tcgttaaccg
     2041 gatgaattac atccgttccc aaatccactc cctggcattc accaatgctt cgatccaggg
     2101 cgaaacttca tcgttccgcc tgtcttcggg ataatacgcc cattgccagg gatgcatggc
     2161 cctttcggga tgcggcatga tagccaggtg gcggccatcg tccgatgcca cacaggcggc
     2221 gtcgaacatt gatccgttgg gatttcccgg caaggtggaa tacgaatact ttgccggaat
     2281 gttgtatctg gattcttcat acgggaacat gaacctgcct tcgccatgcg ccacccagat
     2341 tcctaatcgc gttcctgcca gcgacttcag catcaccgaa ttgttttcaa gaatatcgat
     2401 ggtaaggaac gacgattcgt atttgccagc gttatgcaac agcttatgct tcttttcgtg
     2461 accgggataa atgagaccca gttccgccat caactggcag ccattgcata ctccgagcga
     2521 aagcgtatcc tcacgggcat aaaatcggtc gagggcttct ttggctttcg ggttgtaaag
     2581 aaaagctccc gcccagccct tggcagaacc aagcacatcg ctgttggcaa agccgcccac
     2641 aaacacgatg aaattcacat cttcgagcgt ttcgcggccg gtgataaggt ccgtcatatg
     2701 aacatccttc acatcgaagc ctgccagatg catggcccag gccatttcgc ggtcgccgtt
     2761 cgaacctttt tcacggatga tggcagccgg aatcccggta cgggtgcggc ggtccggatc
     2821 gatgccgtat tgttcacagg taccggtgaa actgctgttg aacctgaact tcctttcggc
     2881 tgttgcataa tgacgaaaac gctcaagcgc aaaatcttcc ccgctttgct tcctgtcgag
     2941 aagataagaa gtacggaacc agtcgtcgcg caatgcatcg atgccaaaca cccattcttc
     3001 gcccgaatta acgatcttca cctcccgtcc ggcaaccggt tttccgatta ccgaaaattc
     3061 aatacccctt tcatgaagga aaatgcttac aaaatccagg tcgttggttt gaataacgac
     3121 ggcaggcttt tcgctgaaca acagccttac aatatccttt tcgcctaggg ccgaaagatc
     3181 gatgccgagg ccgagcgtgt tgcggctgaa agtcatttcg agcaatgtcg tgatcaaacc
     3241 gccggctgaa acatcatgcc cggccaggat catctcttta ccgatcagtt cctggaccgc
     3301 cgcaaaagcc ttacggaagt atgcagcatc atccacggtg ggcgttgtct ttcctatccg
     3361 gttcacggcc tgtgcaaacg aactgccgcc caattcaaag gcgcttttcg agaaatcgat
     3421 ataaacgagc gaggaggtga gctgctcttt cagcaccggc gagattactt ttctgacatc
     3481 ggttacctcc cctgccgcgg aaatgataac ggttcccggt gccaggacga cttccccgcc
     3541 gggatatttc tgcgtcatcg atagcgaatc cttgccggtg gggatattga tccccaatgc
     3601 aatcgcgaaa tcgctaatgg ctttcaccgc cgaataaagc ctggcatcct cgccttcgtt
     3661 cctgcagggc cacatccagt tggccgacaa cgaaacgccg tcaaggccct gttcgatggg
     3721 cgcccaaacc aggttggtca gcgcttcggc aaccgacagc accgatcctg ctgctgcatc
     3781 aatcatcccg ctcacgggag catggccgat ggaagtggca atgccttttg tcccggtata
     3841 atccagtgat acaaccccga catcattcaa aggcaactga agcgggccgg cacactgctg
     3901 gcgggcgata cggccggtaa ccgaacggtc cgccttattg gtaagccagt ctttacaggc
     3961 aaccgcttcg agctgcagaa cccggcttac atattggtaa atattctctt tatcgtaagc
     4021 aacatcccgg tacgcttcct gcttccggac gtcgtgcatg atggttaccg gcggtttccc
     4081 gaaaagatat tccagccgga tatcgagcgg tttttcaccg gtttggggat tcacgatgcc
     4141 gaattgcata tcgccggtaa tttcccctac ttcgtacatg ggcgcgcgtt cacggtcggc
     4201 gatgcggcgc agcaaggcgg catctttctc tttgatgacc agccccatgc gctcctggct
     4261 ttcattaccg atgatttctt tagcagaaag agtcgggtcg ccgaccggca atttggtaat
     4321 gtctatttgt cctccctgct cttccaccag ttccgacagc gaattgagat gccctccggc
     4381 gccatgatcg tggacggaga tgataggatt tgcatcgctt tcggccaaag cacggatcgc
     4441 gttataaacc cgtttctgca tttcgggatt tgaacgctga acggcattca gttcgatggc
     4501 gctgtgatat tcgccggtgg ccacgctcga cacggcgccg ccgcccatac cgatgcggta
     4561 attatcgccg cccataacca ccagtttatc gccgggctgg ggatgtccct tcatggcatc
     4621 ctcttttcgg gccgatccga tgccgccggc aagcatgatc accttgtcgt aacccgattt
     4681 tttcaggttc tcgaaatgtt cgaaagtaag gacagagccg cagatgagcg gctggccgaa
     4741 tttattcccg aaatcgctgg cgccgttcga agctttgatc aggatttctt cgggggtttg
     4801 atataaccat tcgcggggtt ccgttgcttt ttcccattca tgctcttcag ccgtcctggg
     4861 ataggaagtc atgtaaaccg ccgtaccggc gatgggcagc gagcccttgc cgccggccat
     4921 ccggtcgcgg atctcgcccc cggaaccggt agccgccccg ttaaaaggct caaccgtggt
     4981 ggggaaattg tgcgtttcgg ctttcagcga tatcaccgag tcgatgtctt tgacttcgaa
     5041 aacatcgggc ttgtcctggg ttgccggggc gaactgggtg accggcggac cgtcgataaa
     5101 agccacatta tctttatagg ctgaaacaat gcgtcccggg tgggctttcg acgttttttt
     5161 tatcagctga aagaggcttt caggcttttc ttccccgtcg atgacaaatt tcccgttgaa
     5221 gatcttatgc cggcaatgct ccgagtttac ctgcgaaaag ccaaagactt cgctgtcggt
     5281 aagtttgcgg cccaatttac ggcttacctc atccaggtac ccgacttcct gttcgctcag
     5341 cgccaggcct tccttccggt tatattcggc gatatcgccg atatacataa caggttcagg
     5401 ttccttatgg attgtaaaaa tttcctggtc cagcccgtga tacatgcgct gcagcatatg
     5461 gtcatacggc gctttttcac cggcaacttc ggtaaactct tcgatgcgcg ctatccccga
     5521 tattcccatg ttttgggtga tctccacggc attggtactc cagggagtga tcatttcgcg
     5581 ccgcgggccg acgaaaaacc cggctaatgt ctcctgatcc acaagccggg cttcgctgaa
     5641 aagccattct agttttgaaa tatcatcggg ctgtgaaccg ccgctatact gaactgcata
     5701 agcagtttta ccggaacgga aaaattggac cataactttc attttcgatg cacaaagata
     5761 ctgaaacaag aagaatgaga agttaagcaa atatgattct atcaaacagg tatggcgtag
     5821 ctgtttctct gtttccgggc atcgtttagc aagcctcccg ataaactatt gaatttcatt
     5881 attttggaag tgtcaggaaa aagaattact tttgccgcaa ttaattcaat gatctgcgcc
     5941 attgcaccac gaatataaag ctatagcttc ctgcgtttta caaaatctgc agatcttcat
     6001 cctttatcat tacccacgtg aatttttcag aatttaattt aaacccgcaa cttctgcggg
     6061 cagccgccga actgggcttc gaaacaccta tgcctgtaca gcaggaagtt attcctttgt
     6121 tcctgggcac cgaccgtgac cttgtcgggc tggcccagac gggaaccgga aaaaccgcag
     6181 ccttcgggtt gccgatcctt cagcttattt cgcccgaaac aaaatcggcc caggcgctta
     6241 tcctggcgcc cacccgcgaa ctgtgcatgc aaatagccgg cgacatccgc gattatgcga
     6301 aacacatgcc gaacatccgc attaccccca tatacggcgg cgcaggattc cagggtcaac
     6361 tgaaagagct tgccgccggc ccccaggtaa tcgttgccac ccccgggcgt atgctcgata
     6421 tgattaaaag gggaaacatc aggctcggca atatccggtt cctggtgctc gacgaagccg
     6481 atgagatgct caacatgggc ttcgaagaag acgtgagcgc catcatcagc aacacgccgg
     6541 ccacaaggcg tactttcctg ttctcggcaa ccatgccggc tcaggtggaa caaatcgcca
     6601 tgaagtacat gaagtcgccc gaaaccgtca ctgtaggcca gcgcggcaaa ggagcgtcta
     6661 cggtaaaaca tttgtacctg attgcccatg ccagcgaccg ttacaaagta ctgaaacgca
     6721 tcgccgacta ttatccgaat atatatggta ttgttttctg ccggacgcgt caggaaacgc
     6781 aggatattgc cgattcgctg atccgcgacg gatacagcgc cgaagccctt catggcgacc
     6841 tgagccagag ccagcgcgat tacgtgatga acaaattcag gataggccac ctcagaatgc
     6901 tggtcgccac cgatgtcgcc gcaaggggaa tcgacgttga agaactgacg catgtgatca
     6961 actataacct tcccgacgaa ctcgaagtgt atgtgcaccg gagcgggcgt accggcaggg
     7021 ccggacgggc gggtatcagc atatccatca tcaacctgaa ggaaaaacat aagatccgcc
     7081 agcttgaaaa agaggtcggg aaagaattct cgcaggtgag gatacccaca ggtcaccaga
     7141 tttgcgagaa gcaactgtac catgtgatcg agaaaatgga aaatgtggag gtaaacgagg
     7201 aagaagtgga agaattttta ccggtcatct ataagaaact caactggatg acgcaggaag
     7261 aactcatcaa acgcttcgtt tcggtagaat tcaaccgttt cctggattat taccgcgacg
     7321 ccggcgacct gaacgtggac gagagcaggg attcccaggg ctcaacaaaa ttcgccaggg
     7381 ttttcatgag cattggccgt aaggacgggc tcattccccc gcggctgatc ggtgtgatca
     7441 acgacttcac cggcatccgg gatatgaaaa tcggcaagat agagatcggc gatacttttt
     7501 cattcgttga aatcgagagc cgttataccg atcagttcat gcaggctttt aaaggcaagt
     7561 tttacaacaa caggccggta agggtcgacg aagccgaagg gcagcataaa aagagaagga
     7621 ccggcgaaaa accgcgaaaa gagactaaat ccgggcctga taacaatacg gccttttcaa
     7681 acaaggatag aaggaaaaag tggaaaaccg aaagcgaccc ttatttcggc gacaaaaacc
     7741 cgaaagaagg gagaaggaag aagaggtttt aattggtaaa tgaaaggatt aggtaaaaac
     7801 aacaaggctt tcgcagataa cggaagcctt tttgttttat cggagtggtc agacgcgagc
     7861 tgacggccat cctgctcccc accgggatag gtcagcaaac tacttacacc tgtgctcccc
     7921 gctcgcgcgc gtttaccttc ggtgagatcg cttcgctaag ttacaacgcg tgtagggtat
     7981 ctttctttat tattttacta tcactttact cccaccgcta ttttgccttg gtacaacgca
     8041 acta
//
LOCUS       DAOPYV010000105         7694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_11448_length_7694_cov_12.684514, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000105 DAOPYV010000000
VERSION     DAOPYV010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11448_length_7694_cov_12.684514"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PKI35_10930"
     CDS             complement(<1..1129)
                     /locus_tag="PKI35_10930"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNX44939.1"
                     /translation="MLFLFCRSASAQQVTIRGKVVDSETLSPLAFVNIVANNGSTGAC
                     TDIDGKFTLKSATPVKILKLTYVGYFNKQVSVDSLPSDKIIKMQKTEVELQEVVIRPG
                     INPAHRIIGEVLANRNLNDHEKLESFSYTSYEKTIFGPDKDSIPMVDSLAADTAFLHM
                     KRLFDSQHLFLMESVVERKFMYPDKNYNNVLASRVSGMSDPLFVFMMSQLQSTSFYRE
                     IIKIGDKEYINPISNGTFTKYYFEILDTLVEPHPYDTTFIISFRPLLNTNFDGLKGVI
                     SISTNNYAIRNVIAEPSRPTGIFTIKIQQLYEFVGDEHWFPLQLNTDLIFKGAIGKNT
                     IEIGAGPGPVDSTRIDLVGRGKSYISDIKLNPPLKTNRFGYI"
     gene            1452..2291
                     /locus_tag="PKI35_10935"
     CDS             1452..2291
                     /locus_tag="PKI35_10935"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNX44940.1"
                     /translation="MEKQLLEYCLHQHRNIGHYLNLCIECAEAESVHQLRVSIKKFRT
                     VNKILGYLIPESSDENARQLAMLRRLFKLAGKLRDAQIQMKILEEYKSKLDIDTALLE
                     NLLAANERNTLKKFVSGYAKFSVALDDEPYQKSGSRILSGSYDGSFSDKAVGLLNKRI
                     DKTRKAVEHADSDEDLHRVRILLKQIRYIHGFLSKQDSFPEGGSESLDGMRNAEILLG
                     RWHDLTVFCDTLKEYTNPKQTKNPDFLKDVRTLNDAVACDIDSFRKQAYALLRQSFGF
                     KAA"
     gene            complement(2278..2610)
                     /locus_tag="PKI35_10940"
     CDS             complement(2278..2610)
                     /locus_tag="PKI35_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNX44941.1"
                     /translation="MTLKQAQETVDQWINTTGVRYFNELTNTALLMEEVGEVARIMAR
                     RYGEQSEKESDKMANLADEMADVLFVLICLANQTGVDLTESFHKNLEKKTSRDASRHQ
                     NNTKLRQP"
     gene            complement(2749..3444)
                     /locus_tag="PKI35_10945"
     CDS             complement(2749..3444)
                     /locus_tag="PKI35_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX44942.1"
                     /translation="MEPKARILLVEDDVNLSDVLTDFLEMTGYSVVLAADGQQGLAEY
                     GKGKFDLLILDVMLPVMDGFSLAAAIRKADGKVPIIFLTARGQMEDRIAGFKAGCDDY
                     ITKPFSSEELSLRIDAILRRCRLEAISNDENYKLGSFIFDPANFELKRANKRINLTPK
                     EAALLKVLCEHKNSLLTREKALKVVWGDDDYFIGRSMDVFITKLRKLLREDPDVAIVN
                     VHGAGFRLEVKEG"
     gene            complement(3413..4738)
                     /locus_tag="PKI35_10950"
     CDS             complement(3413..4738)
                     /locus_tag="PKI35_10950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX44943.1"
                     /translation="MKRNNFLLIVILSALSLAGVVFFQFFWMRSAIQLQEDQFDNKVK
                     LALKGIVNELYECKNDTCTSRDTCSFRCATAHRPNTSHINTRLLDSLVRTEFVSMKLE
                     GPYIYGIFHQGNNKVIYVSNPAYESRLPGTAHYSSLSCIFSKSDYYLGAIFPNEHKLI
                     FNKVLWWTILCFFLIAILIFGFSFTIWSFLRQKRLTEIKNDFVNNMTHEFKTPIATIS
                     LASELLQKPAILASPSRATKYASIIYDENQRLKNQVEHVLQIAVLDRHSYNLKLSEFD
                     LHELINHVVENFRIVLKERKGKLELFTDAENSILVADKMHVQSMLMNLLDNANKYSAS
                     EPSITIRTSNSADGIVFSVEDRGIGISAENQRYIFKKLFRVPTGDVHDVKGFGLGLFY
                     VKNMAEAHGGSIKVKSEPGHGSRFDVYLPFKQPVQTPETDGTKSQNTVG"
     gene            5072..5491
                     /locus_tag="PKI35_10955"
     CDS             5072..5491
                     /locus_tag="PKI35_10955"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNX44944.1"
                     /translation="MPVSVIRYTGNLRNVATHLRSGSEIITDAPIDNQGKGEAFSPTD
                     LLATSLGACAITVIGIAAKTHGFTVDGTEVSVNKIMAENPRRVAGVVVEFNFPPEHYS
                     EKQKQIIRRTADTCPVRLSLHPDLKQTFIFNFFDTHE"
     gene            5484..5861
                     /locus_tag="PKI35_10960"
     CDS             5484..5861
                     /locus_tag="PKI35_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNX44945.1"
                     /translation="MNKIVPGIAGFYEITVTPGMLATAYSSGTLDVFATPSLIAFMEK
                     TAMESVAGYLPENQTTVGTEVHVTHNKVTSVGKKLSFKSLLTETNDRQLVFHVEAYEG
                     DTLIGTGTHTRFIVDTERFLRKL"
     gene            5900..7669
                     /locus_tag="PKI35_10965"
     CDS             5900..7669
                     /locus_tag="PKI35_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNX44946.1"
                     /translation="MNQLSENETFSQQVLIAKTIAGLEDVLAEELTALGAQNVQKLNR
                     AVSFEGDKKLLYRTNYSLRTALRILVPLHTFELSNEDDLYRNIYDYPWENHLKVTDTL
                     AVDAVVTDSELTHSHFVALRTKDAICDRFRASNNGRRPSVDTDNPGFRINIHINGSQC
                     DVLADSSGASLHKRGYRISNAEAPMSEVLAAGLILLSDWDKKSNFIDPMCGSGTLLIE
                     AALIANNFPPGMYRKDFGFMHWNDFDTELWESVKQEAMDEQTEFEYKILGSDISPKNL
                     SSAHANIKSAKLHKDIVLQVSAFENLKPPKDEPGIIVMNPPYGERIRPNDIIGLYRSI
                     GDTLKQSFTGYNAWIISSDQTAIRFIGLKPTQKFIVWNGPLECKFEKFEVYRGSKKYG
                     DEPDEGHISRPPRRQETDSVRGYEERPEGEQFKKKPFNNSRRDDRQGFSREKSHREPH
                     TDGKADDRKKSSRTFDTDENGRRIYTNKRDERKKLYTGRDSDEHRGERKEWSDKRPGN
                     TQDRPFHHFEKKEHREIEEWTPRERPEPKKRIERSDRPKKEEKFEPSQKPKRPRSVKP
                     PKPPQKPGSKGFESGDKWEEMGD"
BASE COUNT         2189 a         1865 c         1707 g         1933 t
ORIGIN      
        1 caatataccc gaaccggttt gttttaagcg ggggattgag tttaatatct gaaatatagc
       61 ttttccccct gccgaccaga tcgatgcggg ttgaatcaac cggacccggc ccagcgccaa
      121 tctcaatggt atttttaccg atggctcctt taaaaatcag atcggtattc aactggaggg
      181 ggaaccagtg ttcatccccg acaaattcat aaagttgctg gatcttaatg gtaaatatcc
      241 ccgtcggcct cgacggttcg gcaatcacat tcctgatggc ataattgttg gtactgatgg
      301 aaatcacccc tttcagcccg tcaaaatttg tattcagcaa aggcctgaac gaaataatga
      361 aggtggtatc ataaggatgc ggttcaacca gcgtatcgag gatttcgaaa taatactttg
      421 taaacgtccc gttgctgatg ggattaatgt attccttatc gccgatcttg ataatctctc
      481 tgtagaatga ggtggactgc aactgagaca tcatgaatac aaagagcgga tcgctcattc
      541 ccgataccct ggaagcaagg acgttgttat agtttttgtc gggatacata aacttgcgtt
      601 ctactacgct ttccatcagg aaaagatgct ggctgtcgaa aagccgcttc atgtgcagga
      661 atgccgtatc agcagcaagg ctgtcaacca tcgggatgct gtccttatca ggtccgaaaa
      721 tggtcttttc ataagacgtg taggaaaagg attccagctt ttcatggtcg ttgaggttgc
      781 ggtttgccag cacctcgcca ataatgcggt gggcagggtt aatcccaggc ctgataacca
      841 cttcctgtaa ttcaacctct gtcttctgca tttttattat cttatcggaa ggcagggaat
      901 caaccgacac ctgtttgtta aaataaccga cataagtcag ttttaaaatt tttacaggcg
      961 tagcggattt cagggtgaac ttgccgtcga tgtcggtaca ggcgcccgtg gaaccgttat
     1021 tggcaacaat atttacgaat gccagcggag aaagcgtttc tgaatctaca acctttcctc
     1081 tgattgtaac ctgttgtgct gaagccgacc ggcaaaatag aaacagcaat gctaaacata
     1141 aaactaagtt ttgtagggca gaaaaaactg atctgaaaat cataaatttt tatcacttat
     1201 aaggaaacaa agttataaac gctttctgca acatagtatt gtaaaataca taacattgca
     1261 aatttagtca ttactaaata aagaacgggt tgattcattg aaacgacctc tgcccctctc
     1321 agcgaggggg tgccaatatc ccgcccctat aaaacttttg acaaatttag tttgattcat
     1381 gagccgccgc gataaaatac tacatgaaaa cgtaatttta catttttatt atgccgggac
     1441 aggctctgat tatggagaag caattactgg aatattgcct gcatcagcac cggaatatcg
     1501 gtcattatct caatctctgc attgagtgtg ccgaagccga atcggtgcac cagttgaggg
     1561 tgagcattaa gaaattcagg acagtaaata aaatccttgg atatctgata cctgaaagca
     1621 gcgatgaaaa cgcccgccaa ctggccatgc tcaggagact ttttaaactt gccggaaagt
     1681 tgcgggatgc gcagatccag atgaaaatac tggaggaata taaaagcaag ctggatattg
     1741 atacggcact gctggaaaat ttacttgccg ccaatgaaag aaatacctta aaaaaatttg
     1801 tgtccggcta tgcaaagttc agcgttgcac tggacgatga accctaccag aaatcaggca
     1861 gccggattct ttccggatct tacgatggtt cgttcagcga caaggccgtc ggattgctga
     1921 ataaaagaat tgataaaacc cggaaagccg ttgagcatgc cgacagcgat gaagacttgc
     1981 atcgtgtaag gatattgctg aaacaaatcc gttacatcca cggcttttta agtaaacagg
     2041 attctttccc tgaaggtggc agcgaatccc tggacgggat gagaaacgcg gaaattctgc
     2101 tcgggcgatg gcatgacctg acggttttct gcgataccct taaagaatac acaaacccga
     2161 aacaaacgaa aaaccctgat tttttaaagg atgtcaggac gctgaacgat gctgtagcct
     2221 gtgatattga ttcctttcgc aaacaggctt atgctttgtt aaggcaatcg ttcggattta
     2281 aggctgcctg agttttgtat tattctgatg cctggaagca tccctggatg ttttcttttc
     2341 caggttttta tgaaaggatt ccgtcaggtc gacacctgtt tggttagcca gacatatcag
     2401 cacaaaaagc acatcggcca tttcatcggc aaggttcgcc attttgtccg attctttttc
     2461 cgattgttcg ccgtatctgc gggccataat acgcgccacc tcacctacct cttccatcag
     2521 cagggctgta tttgtcaatt cattgaaata tcttaccccc gttgtattta tccattgatc
     2581 aacggtttcc tgggcttgtt ttagagtcat gcctgagtaa atctatacaa gttgtgcatt
     2641 tggtattatt gcggattctt acaaacggat gtcgttttaa tctttcaggg agctcccttc
     2701 cgttgaaaac ccgtcgcatc cgtccaatcc gcgcaacgga tcctcatgtt atccttcctt
     2761 cacctccagc ctgaaaccgg cgccgtgaac attcacaata gcgacatcgg gatcttctct
     2821 cagaagtttc cgcagtttgg taataaacac atccatactc cgcccgatga agtaatcatc
     2881 gtcgccccaa actactttca gcgccttttc gcgggtcagg aggctgttct tatgctcgca
     2941 gagaactttg agcaaggcag cttctttagg cgtgaggtta atacgcttgt tggcccgctt
     3001 taattcgaaa ttggcagggt cgaaaataaa acttcccagt ttgtaatttt catcattgct
     3061 gattgcctct aaacggcacc ggcggagaat ggcatcgatg cggaggctga gttcttcgct
     3121 gctgaaaggt tttgtgatgt aatcgtcgca tccggcctta aatccagcaa tccggtcctc
     3181 catttggccc cgggccgtga ggaaaataat cggcactttc ccgtccgctt tcctgatagc
     3241 agccgcaagc gaaaacccat ccataacggg caacatcaca tcaaggatca gaaggtcgaa
     3301 tttccctttg ccgtattctg ccagtccttg ctgcccgtcg gctgccagca ctaccgaata
     3361 gcctgtcatt tcgaggaagt ccgtcaaaac atcgctcagg ttgacatcat cttcaaccaa
     3421 cagtattctg gcttttggtt ccatccgttt caggtgtttg aacaggttgt ttgaaaggca
     3481 ggtaaacatc gaaacggctg ccatgccccg gttcgctttt tactttgatt gatcctccat
     3541 gtgcttcggc catatttttg acataaaaca aaccaagccc gaaccctttg acatcatgta
     3601 catcgccggt aggcacacga aataacttct taaatatata tctttggttt tcagcgctga
     3661 tgccgattcc cctgtcttcg accgagaaaa caatgccatc ggcagaatta ctggtcctga
     3721 tcgtgatgct gggctcggaa gcagaatatt tattggcgtt atccaacaga ttcatcaaca
     3781 tgctctgaac atgcatttta tcggccacca ggattgaatt ctctgcatcg gtgaacaatt
     3841 ccagtttccc tttccgttct ttcagcacaa ttctgaagtt ttcgacaaca tggtttatca
     3901 gttcatgcag gtcaaactcc gagagtttga ggttatagct gtgtctgtct aaaacagcta
     3961 tctgtaaaac atgttcaacc tgatttttca gccgttggtt ttcatcgtaa ataattgaag
     4021 cgtacttggt ggccctggaa ggcgaagcaa gtatcgccgg tttctgcaac aattcactgg
     4081 ccagcgaaat ggtagctatc ggcgttttga attcgtgcgt catattattc acaaaatcgt
     4141 tctttatctc cgtcaaacgc ttttgtctca ggaacgacca aatggtaaaa gaaaaaccaa
     4201 aaatcaggat cgcgatcaga aaaaaacaaa gaattgtcca ccaaagaact ttattaaaaa
     4261 tcagtttatg ctcgttggga aatattgcgc caagatagta atcactttta ctgaaaatgc
     4321 aggaaagcga agaatagtga gcggtacccg gcagccggga ttcataagcc ggattgctta
     4381 catagatcac cttgttgttt ccctgatgaa aaatcccgta aatatatggc ccttcaagtt
     4441 tcatactaac aaattcagtc cttaccagcg aatccagcaa gcgtgtattt atatgggaag
     4501 tattgggccg gtgggccgtg gcgcacctga atgagcaagt atctcttgag gtgcaggtat
     4561 catttttaca ttcatacaat tcgttcacga tgccttttag cgccagtttt accttgttgt
     4621 cgaactggtc ctcctgcaat tgaatggcac tccgcatcca gaaaaactgg aaaaatacga
     4681 caccggccag ggagagcgct gatagtatta cgatcagaag gaaattattc cgtttcatgc
     4741 tccaaagata gtaacaagga gaaacataaa aagattggtt aacaagttat taacaaaata
     4801 agcatgaaat gtcaaacgat ctctgttaat taacaaatgt aagtgtaatc aggatccata
     4861 aataggatca tctgacaacc ggtattggat ggtcccggcg aaggcttcct tctatatgag
     4921 tattccccat ccggtaaaaa gctcagcatc cggcgttaac gaagctgtga aatatccgga
     4981 ctgcctgcat agggcgcccg acatttccct tctcttcaga aaccgcacct tataaatgac
     5041 tacctttgca ctccgaaatc atcacccata gatgcctgta tctgtaatac gctataccgg
     5101 taatttacga aacgttgcca cgcacctgcg ttccggttcc gaaattatca ccgatgcccc
     5161 catcgataac cagggaaaag gcgaagcctt ctcgcccacc gacctgttag ccacctcgct
     5221 gggcgcctgt gctattacgg taatagggat tgcagcaaaa acgcacggtt tcaccgttga
     5281 cggaacggaa gtatcggtaa acaagatcat ggctgaaaat ccccgccggg tagccggggt
     5341 agttgtggaa tttaatttcc cgcctgaaca ttattccgaa aagcaaaaac aaatcatccg
     5401 ccgcactgcc gatacctgtc ccgtaaggct cagccttcat cccgacctta aacagacctt
     5461 catttttaac tttttcgata cgcatgaata aaatagttcc cggcatcgcc ggcttttatg
     5521 aaataaccgt aactcccgga atgcttgcaa ccgcttacag ttcaggtaca ctcgacgtat
     5581 ttgccacccc atcgctgatc gccttcatgg aaaaaaccgc catggaaagc gtggccggat
     5641 atctccccga aaaccaaacc accgttggca ccgaagtaca tgttacgcac aacaaggtta
     5701 cctcggtcgg caaaaaactt tctttcaaat cattgcttac cgaaaccaac gacaggcagc
     5761 ttgtttttca tgttgaagct tacgaaggcg ataccctcat tggcaccggg acacacacaa
     5821 ggtttatcgt ggataccgaa cgtttcctcc gtaaactata accgttttac cgaaatacca
     5881 accttctaaa tacagtcctt tgaaccaatt atccgaaaac gaaacttttt cgcaacaagt
     5941 acttattgcg aaaaccattg ccggactcga agatgtcctt gccgaagaac ttactgctct
     6001 cggggcgcaa aatgttcaga aactgaacag ggctgtcagc tttgaaggcg acaaaaagtt
     6061 gttataccgg accaattaca gtcttcgcac cgcgttgcga atacttgttc cgttgcacac
     6121 cttcgaactt tccaacgagg acgaccttta ccggaacata tatgactatc cctgggagaa
     6181 tcatctgaag gtaaccgata ccctggcagt ggatgccgtt gtcaccgatt cggaactcac
     6241 ccattcacat tttgtggcat tacgcaccaa agatgccatc tgcgaccggt tccgcgccag
     6301 caacaacggg cgcaggccct cggtggatac cgacaacccc ggtttccgca tcaacattca
     6361 cattaacggc agccagtgcg atgtactggc cgacagttca ggagcttcgc tgcataagcg
     6421 cggttaccgt atttccaatg ccgaagcccc catgagcgaa gtgctggccg ccggcctgat
     6481 cctgctttcg gactgggata aaaagtcaaa tttcattgat cccatgtgcg gatcggggac
     6541 tttactgatc gaagcagccc ttatcgccaa caattttccg ccgggtatgt accgaaagga
     6601 cttcggtttt atgcactgga acgatttcga cacggagcta tgggaatcgg taaaacagga
     6661 agccatggac gaacaaacgg agtttgagta caaaatattg ggcagcgata tctcaccgaa
     6721 aaatctttcc tccgcccatg ccaatatcaa atcggcaaaa cttcacaagg acattgtatt
     6781 acaggtttcg gcattcgaaa atctgaaacc gcccaaggac gagccgggca tcatcgtgat
     6841 gaacccacct tacggcgaac gtatccgtcc caacgacatc ataggtctat accggagcat
     6901 cggcgatacg ctgaagcaat cgttcaccgg ctacaacgca tggatcatca gttccgacca
     6961 gacggccatc agattcatcg gtctgaagcc aactcagaaa tttattgtct ggaacggtcc
     7021 gctggaatgt aaattcgaga aattcgaggt ataccgggga tcaaagaaat acggcgacga
     7081 accggatgaa ggtcatataa gcaggccgcc acggcgtcag gaaacggatt cggttcgcgg
     7141 ctatgaagaa agaccggaag gcgaacagtt taagaaaaag ccttttaaca atagccggcg
     7201 ggacgacaga caaggcttca gcagggaaaa aagccaccgg gaaccacata ctgacggtaa
     7261 ggccgatgac cgaaaaaaaa gcagcagaac tttcgatacc gatgaaaacg gacgccggat
     7321 ctataccaac aagcgcgacg aacgcaaaaa attgtacacc ggccgcgatt cagacgaaca
     7381 ccgcggagaa cgtaaagaat ggtccgataa gcgcccggga aatacccagg acaggccttt
     7441 ccaccatttt gaaaagaagg aacaccgcga aatagaagaa tggacgccgc gcgaacggcc
     7501 tgaaccaaag aaacgaatcg aaagaagcga ccgcccgaaa aaagaagaaa aattcgaacc
     7561 atcgcaaaag ccaaaacggc cccgttcggt aaaaccgccc aaaccgccgc aaaagcctgg
     7621 atcaaaaggt tttgaatcag gcgacaagtg ggaagagatg ggagactgac ccccgcgcca
     7681 tgcccctagc tttc
//
LOCUS       DAOPYV010000106         7546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_11786_length_7546_cov_12.313176, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000106 DAOPYV010000000
VERSION     DAOPYV010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11786_length_7546_cov_12.313176"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1136)
                     /locus_tag="PKI35_10970"
     CDS             complement(54..1136)
                     /locus_tag="PKI35_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNX44947.1"
                     /translation="MNKNSSIYVAGHKGLVGSAIVNALQGKGYNNLILRTHSELDLTD
                     QQKVKSFFEKEKPEYVFLAAAKVGGIMANSLYRAQFIYENIQIQNNVIHNSWSSGVKK
                     LLFLGSSCIYPRLAPQPLKEEYLLTDELEYTNEPYALAKIAGIRMTESYNLQYGTNYL
                     AVMPTNLYGLNDNFHLRRSHVLPAMIRKMHLAKCLEQGNIDAIRTDLLRHPMEDIRHD
                     APEAALTAFLNSQGISGTGNTATLTLWGTGTPLREFLWSGDMADACVFVMENVDFSDL
                     AKESAPEIRNTHINIGSGKEITIRELAYLVKKIVGFDGKIAFDASKPDGTPRKLLDVS
                     KLEKLGWKYTVELDDGIRKVYDNYRR"
     gene            complement(1259..1618)
                     /locus_tag="PKI35_10975"
     CDS             complement(1259..1618)
                     /locus_tag="PKI35_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX44948.1"
                     /translation="MRNFRNLNIWKSAICFAAQTYEVTQTFPKSEVYGLSHQLQRASV
                     SIASNIAEGSSRKSENEFAHFLEISIGSAFETETQMIIANELNYLSNDQLDVILKSLH
                     ELQKQINQLIIVLRKNK"
     gene            complement(1795..2904)
                     /gene="gmd"
                     /locus_tag="PKI35_10980"
     CDS             complement(1795..2904)
                     /gene="gmd"
                     /locus_tag="PKI35_10980"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNX44949.1"
                     /translation="MKTALITGITGQDGAYLAEYLLKKGYTVHGLKRRSSLFNTDRID
                     HLYQDPHTENRNLILHYGDMTDSSNLIRIVQEVQPDEIYNLAAMSHVKVSFDTPEYTA
                     NADALGTLRLLEAVRLLGLKDKTRIYQASTSELYGLVQETPQTERTPFYPRSPYAVAK
                     LYAYWITVNYREAYGLFASNGILFNHESPIRGETFVTRKITMAAARIALQMQEKLYLG
                     NLSAKRDWGHAKDYVRAMYKVLQHEKPDDFVIATGKTTEVRQFVSMAFAELGIELRFE
                     GEGLNERGIVAGCTGEYQLPAGKVVVQVDPAYFRPTEVELLLGDPSKANNLLGWQPEH
                     DLRSLVQDMMQSDLKLAKKDKHLKDGGFRVNNYFE"
     gene            complement(3027..3944)
                     /locus_tag="PKI35_10985"
     CDS             complement(3027..3944)
                     /locus_tag="PKI35_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX44950.1"
                     /translation="MDQPFNFGLIGAGGYIAPRHLKAIKDTGNILVAALDKYDGVGIM
                     DSYFPECNFFTEPERFDRHLDKLRRKGPGQKIDYVSICSPNYLHDAHIRLALRNQANA
                     ICEKPLVLNPWNLDALAELEKETGKRINTIFQLRLHPVIIALKERIAAAPSGKIFDVS
                     LKYITSRGLWYYVSWKGDIQKSGGIATNIGIHFFDMLTWIFGDVKENKVKEHQPDHAS
                     GFLMLEKARVNWFLSINAGYLPESAVAAGKRTYRSITVDGEEVEFSEGFTELHTRSYE
                     EIFKGNGFGLEDARQSIEIVHEIRNQIKY"
     gene            4123..4923
                     /locus_tag="PKI35_10990"
     CDS             4123..4923
                     /locus_tag="PKI35_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNX44951.1"
                     /translation="MSEPITSVQNPRIKNIVLLQSKSRERKLQDRIVIEGYREISRAL
                     ASGVVVKELYYCPELDTGNRVGEVQQHAGQAVFYEISRTVFEKIAYREGSDGLIALAQ
                     PKYLSTEDIQVKENLLLMILESVEKPGNLGAVMRTADAAGVDAVIVCDPLSDIYNPNV
                     IRSSVGCVFTVPVIAGTNEDIWQWLTKNRIKSYAAALTEEAIPYDTVDYSGPSAIVLG
                     TEATGLSRSWLEFSDRQIIIPMHGIADSLNVSTSAAIIIFEAVRQRKI"
     gene            complement(5188..7137)
                     /locus_tag="PKI35_10995"
     CDS             complement(5188..7137)
                     /locus_tag="PKI35_10995"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44952.1"
                     /translation="MKIRLTLKCLPLLFLLSLITAKLPAVYAQGQMPANTGQDSKQGT
                     SKEKPSPDSAFVCYFYQNPQGSYTLKKISSSLYKLQDYDAVQQGESMYASLGNPGSAS
                     SPLSYRFCTVPSFDLWLRNFAPYRFSFDSTRFYISDSPYSRLDYVMGSSKEQRLVVTH
                     SQQVRKGLTLSLNARFANAPGIYLRQRSFYSGVTLNASYFTPSKRYGVVGTYLNDRFR
                     VYENGGLAYDSVFTNNTDSKRQTFLVNLDEAMNRGKSSGFLLQQYFNLQKSQAKEADS
                     LGQPVKTRRFDAGRFVYTFRYSRNSAVYEDIDPDVQYYPAIFLDSLTTYDTAAQIHFE
                     NSLVYSNIEPDTSGKEFPLQYAFGIKHLNDRIVNDSLTSTFTHLVPFGTLRGIIAGKT
                     FFTASGKIYLGDYNNGDYDLSGEFYQYIGRKNEYRLWLSASNGLIHPDYFYQRYLSNH
                     YKWDNDFKAESYQIGTAGIDIKGFRLIATATRVSNFVYLDKTAQPRQLDDGLMFVSFN
                     FSKDLKIGHWIFGSNATWQQTTKDTILSLPAITARLTVCYNMDLFKKALKAQIGFDVR
                     YNTLYYANAYNPVLRSFYLQDEKKYGDYPYGDVFLNFKVKRARIFIKYQHANAGLLGY
                     TYMMVPGYPQADASLKFGISWVFFD"
BASE COUNT         1902 a         1575 c         2014 g         2055 t
ORIGIN      
        1 cgaaaccatg ccgggcatac gatggatatt tctccggcat ctccattacc taattatcgc
       61 cggtaattat catacacctt ccggatccca tcatccagct caacggtgta cttccacccc
      121 agcttttcca gtttgctcac gtcgaggagt ttccttggcg tgccgtcggg tttggaagcg
      181 tcgaaagcga ttttgccatc aaagccgaca atctttttca ccaggtatgc cagttccctt
      241 atggtgatct cttttcccga gccgatgttg atatgggtgt tcctgatttc gggtgcgctt
      301 tcttttgcca ggtcgctgaa atcaacattt tccatgacga agacgcaggc gtcggccatg
      361 tcgcccgacc agaggaattc acgcaagggc gtgccggtgc cccataaagt gagcgttgca
      421 gtgttgccgg tcccggagat gccttgcgaa ttgagaaacg ccgtcagcgc cgcttccggc
      481 gcgtcgtggc ggatatcttc catcggatgc cggagcaggt cggtgcggat ggcgtcgata
      541 ttgccctgtt caaggcattt tgccaggtgc atcttccgga tcatggccgg taacacgtgc
      601 gaccggcgga ggtgaaaatt gtcgttgagg ccatataggt tggtaggcat cacggcgaga
      661 tagttggtgc cgtattgaag attataggat tctgtcatcc ggataccggc gatcttggcc
      721 agggcatagg gctcgttggt gtattccagt tcgtcggtga ggagatattc ttccttaagc
      781 ggttgcggcg ccagccgcgg atagatgcag gaactgccca ggaacagcag ttttttcacc
      841 cctgacgacc aggaattatg tataacgtta ttctgtatct ggatgttctc gtagataaat
      901 tgagcccggt acaggctgtt ggccatgata ccgcctactt tggctgccgc aaggaataca
      961 tattcgggtt tttccttttc aaagaaactt ttaacctttt gttgatcggt caggtccagt
     1021 tccgaatgag tacgaaggat gaggttgttg taaccttttc cctgcaaggc gtttacgatg
     1081 gcgctgccta cgaggccttt gtggcctgcg acgtatattg aggagttttt attcacgaga
     1141 gtcacgaatt gatgctttta attgttgact gcttttggct attggctatt ggctgctggc
     1201 tagaggttgt tgacgattag cgaatactta attttggttt tgatacaaga agtaccaatt
     1261 atttattctt tctgagtact attatgagtt gattaatttg tttttgcaat tcatgcagac
     1321 tttttaggat cacgtcaagt tgatcgttac taagataatt cagttcattt gcaataatca
     1381 tctgggtttc agtttcaaag gctgaaccta tcgaaatttc aaggaaatgg gcaaattcat
     1441 tttctgattt tcgtgatgaa ccttctgcga tatttgaggc gatagaaaca gaggccctct
     1501 gtaactgatg agacaagcca taaacttctg attttgggaa tgtttgagta acctcatagg
     1561 tttgagcggc aaaacagatc gcacttttcc agatattaag attacggaaa tttctcatga
     1621 taaatacgaa ttaagggtta tgttgttggt tgtttggagg ggctaatggg ttatgggtgt
     1681 taggttttgg ctgtctgaga gctaaaggct aaaagccaaa ggccaaaagc tgattgccaa
     1741 attgtttatt tgggaatatt gatgttacat atccggaaat tcgtaaaaaa ccggttattc
     1801 aaaatagtta ttcaccctga agccgccatc cttcaaatgt ttatcttttt tggccagttt
     1861 caggtccgat tgcatcatgt cctgcaccag tgaacggagg tcgtgctccg gttgccagcc
     1921 aagcaggtta tttgcttttg agggatcgcc gagcagcagt tctacttctg tcgggcggaa
     1981 atatgctgga tcaacctgga cgactacctt gccggccggc agttgatact caccggtaca
     2041 tccggccaca attccccttt cattcaggcc ttccccttca aagcggagtt caattcccag
     2101 ctccgcgaaa gccatactga cgaattgccg tacttcggtg gttttcccgg ttgcaatcac
     2161 gaaatcgtcc ggtttttcat gctgtaaaac cttatacatg gcgcggacat aatccttggc
     2221 gtggccccag tcgcgtttgg cggagaggtt gccgaggtac agcttttcct gcatttgcag
     2281 ggcgatacgc gctgcggcca tggtgatctt gcgggtgacg aacgtttcgc cgcgtatggg
     2341 cgattcatgg ttaaacagga tgccgttgga tgcaaacaat ccgtatgctt cacgatagtt
     2401 taccgtgatc cagtaggcgt aaagtttcgc tacggcataa gggctgcgcg gataaaaagg
     2461 agtgcgttct gtttgaggcg tttcctgcac caggccgtaa agttcgctgg ttgaagcctg
     2521 gtagatgcgg gttttatcct tcaatcccag gaggcgcacg gcttcgagca ggcgtaacgt
     2581 tccgagggca tcggcgttgg cggtgtattc aggtgtgtcg aagctcactt ttacgtggct
     2641 catagccgcc aggttataaa tttcgtcagg ctgaacctcc tgcacaatac ggataagatt
     2701 cgatgaatcg gtcatatcac cgtaatgaag gatcagattc ctgttttcgg tatgcggatc
     2761 ctggtacagg tggtcgatac ggtcggtatt gaatagggaa ctgcggcgtt taaggccgtg
     2821 gacggtatag ccttttttca gcagatattc ggcgagatag gcgccgtcct gtcctgtgat
     2881 gccggtgatg agtgcggttt tcatggcgct ggttattggt tttatcaatg aattgtacga
     2941 attacaacac agatgattac gaattataca tcagtgctgt tcataataat ggtgttggtt
     3001 cgtgataaat tcctgcgaat ccggggttaa tatttaatct ggtttctaat ttcatgcact
     3061 atttcgatgc tttgccgggc atcttccaaa ccgaagccgt ttcctttaaa gatctcttcg
     3121 taactccggg tatgcagttc ggtaaaccct tcgctgaatt caacttcttc gccgtcgacg
     3181 gtgatagagc ggtaggtcct ttttccggcg gcaaccgccg attcaggaag ataaccggcg
     3241 ttgatactca ggaaccagtt aacacgggct ttctccagca tcaggaaccc cgaagcgtgg
     3301 tccggttggt gttctttaac cttgttctct tttacgtcgc cgaaaatcca ggtgagcatg
     3361 tcgaaaaaat ggatcccgat gttggtagcg ataccgcctg acttctggat atctcctttc
     3421 caggaaacgt aataccagag accgcgggat gtgatatact tcaggctgac atcaaagatt
     3481 ttcccggacg gggcggctgc tattctttct ttcagggcaa tgataaccgg gtgaaggcga
     3541 agctgaaaaa tggtattgat gcgtttgccg gtttcttttt cgagttctgc caaagcatca
     3601 aggttccagg gattgagtac cagcggtttt tcgcagattg cattggcctg gttgcgtaat
     3661 gccaggcgta tatgggcatc atgaaggtaa ttcggggagc agatggatac ataatcaatc
     3721 ttttgtccgg ggcctttccg tcgaagttta tccaggtgcc ggtcgaaacg ttccggttcc
     3781 gtgaagaagt tacattccgg gaaatagctg tccataatcc cgacgccatc gtatttgtcg
     3841 agggcggcga caagtatatt gccggtatct ttaatggcct taaggtggcg tggggcgata
     3901 tacccgccgg ctcctatcag tccgaaatta aagggttgat ccatagtttt tgatttttaa
     3961 tgaaacaatt ccctgccgga gcaattcagg aatcatacaa gggttcaaag ttaggataag
     4021 ttttataaac atggctatgg gcattgttgt tgattttatt gcggtttgcc tgaaaacaga
     4081 gtatggtttt agctttacat ttgcatagtc ataaacagga acatgtccga acccataacg
     4141 tcagttcaga atccgcggat taaaaatatc gtattgctgc agtcgaaaag ccgggaaaga
     4201 aagcttcagg acaggattgt cattgaagga taccgggaga tttcgcgcgc cctggcttca
     4261 ggggttgttg taaaagaact ttactattgt ccggaattag ataccgggaa ccgggttggg
     4321 gaggtgcaac aacatgccgg acaagctgtt ttctatgaaa taagcaggac tgtgtttgaa
     4381 aagattgcct accgggaagg ttccgatgga ctgatagcgc tggcccaacc aaagtatctt
     4441 tcaacagaag atattcaggt gaaggaaaat ctgttgctga tgattctcga gtcggttgaa
     4501 aagcccggca acctgggagc tgtgatgcgt accgcagatg cagcgggcgt tgatgcggtt
     4561 attgtttgcg atccgttaag cgatatttat aatcccaatg tgatcaggtc aagcgtggga
     4621 tgcgtgttca cagttccggt tattgccggt accaacgagg atatttggca gtggctgacg
     4681 aaaaacagga taaagagtta tgcagccgcc ctcacggagg aagcgattcc ttatgatacg
     4741 gttgattatt cgggaccttc ggcaattgtg ctgggtaccg aggcaacagg cctgagcagg
     4801 agctggctgg aattcagtga ccggcagatc atcatcccca tgcatggcat cgccgattca
     4861 ctgaatgttt caacttcggc agccattatt atttttgaag cggtgaggca gaggaaaatt
     4921 tgaaaatttg aaaatgtgga aatttgaaaa tgagagaaag ggatgtaggc aagctaacag
     4981 ctaagagcca atggtaaggc gaaaagcgga gggcggaggg caggagcaag gagtgaggag
     5041 cgaaggggca aggggcatgg agcatggagc attgagcgag gagccaaagg ccaacagcta
     5101 acagtttacc tgcttgggca ggcagtgcgt aagacggcag gctaatagct aaaggccaag
     5161 agccacggga taactgcatg ggcgctatca atcgaaaaac acccagctta tcccgaactt
     5221 cagcgacgca tcagcctgag gataaccggg gaccatcata tacgtatatc cgagaagtcc
     5281 tgcattggca tgctggtatt tgatgaaaat tctggcgcgt ttcaccttga aattcagaaa
     5341 aacgtctccg taaggatagt cgccatattt tttctcgtcc tgcaggtaaa aagagcgtaa
     5401 gacaggattg tatgcatttg cgtaatagag ggtattgtat cttacatcga aaccgatctg
     5461 ggctttaagg gcctttttaa acaaatccat gttgtaacaa acggttagcc ttgcggtaat
     5521 ggcaggcaaa ctcaggattg tatccttcgt ggtctgctgc caggtggcat tgctgccgaa
     5581 tatccagtga ccgattttca ggtcttttga aaaattgaag gagacgaaca tgagtccatc
     5641 gtcgagttgc cggggctgag ccgttttatc caggtaaaca aaattggata ctctggtagc
     5701 tgtggcaatc aaacggaatc ctttgatgtc aatgcctgcc gtgccgatct ggtagctttc
     5761 tgccttgaaa tcgttatccc atttataatg gttggagagg tagcgctggt aaaagtaatc
     5821 gggatgtatg aggccgttgc tggccgacag ccaaagccgg tactcatttt tccggccgat
     5881 atattggtaa aactctccgc tcaggtcgta atcaccatta ttataatcgc ccagataaat
     5941 ttttccggaa gccgtgaaaa acgtttttcc ggctatgatg cccctgaggg tgccgaaggg
     6001 taccagatgg gtgaatgtgc tggtcaaaga atcgttgaca atacggtcgt tcaggtgttt
     6061 gatcccaaac gcgtattgta acggaaattc ttttccggag gtatcgggtt caatatttga
     6121 atataccagg ctgttttcaa aatgtatctg agcggcagta tcataagtag ttaatgaatc
     6181 gagaaagatg gccggataat actgaacatc aggatcaata tcttcataaa cggctgaatt
     6241 acggctatac cggaaagtat atacaaatct tccggcatcg aagcgacgcg ttttaaccgg
     6301 ttgcccgagg ctgtctgctt ctttagcctg tgatttctgc agattgaaat attgttgaag
     6361 taaaaatccg ctgcttttac cccggttcat ggcttcatcc aggttaacca gaaaggtttg
     6421 gcgtttgctg tcggtattgt ttgtaaatac gctgtcgtat gccagtccgc cgttttcata
     6481 tactctgaaa cggtcgttca ggtatgtgcc gacgacgcca tagcgtttcg aaggcgtaaa
     6541 atagcttgca tttagagtta ctcctgaata aaacgagcgt tgccgtaaat agatgccggg
     6601 tgcattggca aaacgggcgt tcagcgacag tgtaagccct ttcctcacct gttgactgtg
     6661 agtaaccaca agccgctgtt ctttcgaact tcccatcaca taatcgagcc gtgaataagg
     6721 ggaatcggat atataaaaac gcgtactgtc gaaggaaaaa cggtatggcg caaaattgcg
     6781 tagccaaagg tcgaacgacg ggacagtaca gaaccgatag gagaggggag aagaggcact
     6841 tccgggattg cccaggcttg catacatact ttcgccctgc tgaacggcat cataatcctg
     6901 cagtttatat aaggaggaag aaatcttttt cagtgtgtac gacccttgtg gattttggta
     6961 aaagtaacat acaaaagcag aatcggggct cggtttttct ttgctggttc cctgttttga
     7021 gtcctggccg gtattcgcag gcatctgtcc ctgggcatac acggcgggta actttgctgt
     7081 gatgagagac agcaggaaca gaagtggaag gcatttcagt gttaaacgga ttttcatata
     7141 atttgcaaaa ataccgaaaa tagggatata gcgaaggtgt ttaaggtggc tttcatgaga
     7201 tgaatcagaa attgggtagc gataaaggag agagcaacaa cgcgatggat cagggaacga
     7261 tgaaatgaag gacgtgaaag tcgtgaagag gtaaaataaa aaatcttgtc gagaggaaaa
     7321 gggtttagga aagggggtgg cgacatactc tctccgtccg gctccgacgg aagtatcatc
     7381 tgcgctgacg gacttatgct ggttgaattc agtattggaa agggtagagg gagtggtcag
     7441 aaaaatcggt aagggataaa aacaaaaaaa ccctgtcggt aaggacaggg tttaggaaag
     7501 ggggcggcga catactctcc cggggttaac cagtaccatc tgcgcg
//
LOCUS       DAOPYV010000107         7117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_12841_length_7117_cov_12.078873, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000107 DAOPYV010000000
VERSION     DAOPYV010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12841_length_7117_cov_12.078873"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PKI35_11000"
     CDS             complement(<1..450)
                     /locus_tag="PKI35_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44953.1"
                     /translation="MAIENEFPPSWPCSCDTCVSYCRRPGWWTVREAEMAVAAGYAGR
                     MMLEIAPELNFAVLSPAFRGNEGNYALELYAAEGCTFLVNNLCELFGTGLQPLECRYC
                     HHEREGMGENCHQAIEAEWNTDDAKRLIVRWGNLTGFWLKQGMEVHEK"
     gene            831..2657
                     /locus_tag="PKI35_11005"
     CDS             831..2657
                     /locus_tag="PKI35_11005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNX44954.1"
                     /translation="MENFVVSARKYRPVTFDSVVGQQAITGTLKNAIRNNQVAQAFLF
                     CGPRGVGKTTCARILAKTINCQNLSPNLEPCNECDFCKAFNQSASFNINELDAASNNS
                     VDDIRNLVDQVRIPPQMGKYKVYIIDEVHMLSQAAFNAFLKTLEEPPAYAKFILATTE
                     KHKIIPTILSRCQIFDFKRITVDDIAKHLAWVAQSENVSAEPEALHIIAQKADGALRD
                     ALSMFDQMVSLGNHSITYKLVIDNLNVLDYEYYFRMTDYMLAGDIQQSLLLFNDIIDK
                     GFEGQHFITGLGDHFRNLLMCRDASTAKLMETSENIRNRYLSQTKQCPIQFIISALEI
                     NNKSDVNYRTSNNKRLLAEVMLIQLCQLTGANRSSQSGAATVPAAAAPPSPEPVKPAE
                     PEKAKTPVYPASPATEPAKAEESPAQATPTGEKPQPAEDNPAPPSALPDEELHTVTSG
                     KPSGTRTVMPVSLSIKSAISQANQNAAKPEADHHRTSSFTDSQLAESWNTFAAGIKEE
                     STLLYSALTGHLPVIGEGNVLNLELENRVLEAEFNRNKTDLITYLKNSLKNDFISIET
                     SVSEQVTEVKKPYSEKEKFEQMAEKNPLIKTLRDQLDLGLDG"
     gene            2683..3609
                     /locus_tag="PKI35_11010"
     CDS             2683..3609
                     /locus_tag="PKI35_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNX44955.1"
                     /translation="MRRVFTLGETVLDILFRSGAPVNSVAGGSMLNTAVALGRMGCQV
                     EFISEFGQDAPGKIIENSLEKNHVSSKHSIRYDDRFNTSVALAFLDESQNASYTFYHH
                     QPDELTRTTVPETGPGDILAFGSFYSVKPARRRHVAKIVRSANESGAFIIFDPNIRKN
                     HLADLPSIREVFIENMSLSHLVKGSCEDFAFLTGADNPANIYDSLKSYCPYIIITQGE
                     KEVLLFTPRFRKSYPVEIISPISTIGAGDNFTSGLIYGLLQFEQPESSAGNLTLPQWD
                     KLIAYGIAFSAATCLSLENYIPEGFRINLKMQ"
     gene            3606..4178
                     /locus_tag="PKI35_11015"
     CDS             3606..4178
                     /locus_tag="PKI35_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX44956.1"
                     /translation="MKKKFALSLLKLFGWKTSGNLPPDMKKCVVVVAPHTSNWDFFIG
                     FFGYMAIGVKARYLIKKEAFFFPLGPIARALGGIPVDRTRSSNVVPQVVELFRREENL
                     VLTITPEGTRSLVRHWKRGFYLMAEHAGVPLVFGILDYGNKTVGLGPVYYPTGNYDHD
                     IKDIEAYYKDTVARHPENFNLSPQNREKSK"
     gene            4269..5531
                     /gene="tviB"
                     /locus_tag="PKI35_11020"
     CDS             4269..5531
                     /gene="tviB"
                     /locus_tag="PKI35_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HNX44957.1"
                     /translation="MSDIKIALIGLGYVGLPLAVEFGKILPVKGFDINESRIKELEKG
                     HDRTLETSDEDLASATQLTYTHNLDDIRNCNYFIVTVPTPIDKYKRPDLTPLVKASET
                     VGKVLKQGDIVIYESTVYPGATEEECVPVLEKFSGLKFNTDFYCGYSPERINPGDKEH
                     NVTKIRKVTSGSTPEVARKVDDLYRKIIVAGTHMASSIKVAEAAKVIENSQRDINIAF
                     VNELALIFNKLNIDTHEVLQAAGTKWNFLHFSPGLVGGHCIGVDPYYLTHKAQELGYN
                     PEIILAGRRLNDDMGFYSANRTIKLMIHKGHTIAGSSVLVLGITFKENCPDIRNSRVI
                     DVINELKDFGCNVDVYDPWADPHEVKHEYGVEMLPSLDGHRPYDAIVFAVAHREFQQL
                     DLKKFKKENTVLFDIKGTIDRDLVDGRL"
     gene            5555..6436
                     /locus_tag="PKI35_11025"
     CDS             5555..6436
                     /locus_tag="PKI35_11025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX44958.1"
                     /translation="MMQLLTPRHWRDYELIDSGGLEKIERFGKFILIRPEPQALWDRS
                     MSREEWQGLSHAEFRKEKNDPEKGKWILKRGTPEQWEIGYANGEMKLRMRLGLTAFRH
                     IGVFPEQAENWDFIFNTIGSFPKPSPRVLNLFAYTGGASLAARAAGADVLHLDSVKPV
                     VTWARENMELSRLDGIRWIVEDALKFVQREVRRGSKYSGIILDPPAYGRGPEGEKWII
                     DDGLPEMIKHCNQLLEPENSFLILNMYSIGFSALVADNLINSCFSKTGNKEYGELYLS
                     DKFSKRLPLGTFLRFVR"
     gene            complement(6454..6981)
                     /locus_tag="PKI35_11030"
     CDS             complement(6454..6981)
                     /locus_tag="PKI35_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44959.1"
                     /translation="MNKLFSGASAIGIIIVVIGVFFLVRNIFDLDIPVFTILLSAVLI
                     VGGVFMIRGSFAPKHDAQHVQFGDTKFAFTPGQQHYNVLFGEGSLNLKGQKPDSNIPL
                     SVECSFGQFRIIADREVPLKVKGNVSFGTLRGPDMKTASFGEYTYMSPDYNPAQPGFT
                     IDVRVSFGEVEVFYI"
BASE COUNT         1967 a         1833 c         1639 g         1678 t
ORIGIN      
        1 tttttcatgc acttccatgc cctgttttaa ccagaaaccg gtgaggttgc cccaccgcac
       61 gatgagccgt ttagcatcgt ccgtattcca ttccgcctca atagcctggt ggcagttttc
      121 gcccatacct tcccgctcgt gatgacagta ccggcattca agcggctgaa gcccagttcc
      181 gaacaactcg caaagattat tgaccaggaa ggtgcaacct tcggcagcat atagttccag
      241 cgcatagttg ccctcattgc ccctgaatgc cggcgataat accgcgaaat tcaattcagg
      301 ggctatttcc agcatcatac ggccggcata tcccgctgca accgccattt cggcttcccg
      361 taccgtccac cagcccgggc gccggcaata gcttacacag gtatcgcatg aacaaggcca
      421 ggaaggagga aattcatttt cgatcgccat aaaaaagtat caatgaatag aatacgccgg
      481 tattgacatt tcaaacttac aaaatattat cgctaattgc gttatatatt ttctttcatc
      541 cgtttgtaaa taaataaccg gattgataaa tcggaaggct tcacattccg gtcaaccttt
      601 tcggggaatg caggagaaat atcggcagtt tgcgaataaa gaagagccga tcaggattca
      661 ggacggccgg agcagaaaca tcaaggggaa attccttgct caattgccct gttcaaattt
      721 aatcgtgacc ggatattttt tcacaaaatg ttttatcgta cttttgacga tacgggaatt
      781 cccccgttga aaaatcaatc aactgaattt tagaaagata atttctttgc atggaaaatt
      841 tcgtcgtctc agcccgaaaa tacaggcctg taacttttga ttctgttgta ggtcagcagg
      901 cgatcacagg caccctgaag aatgctatcc gcaataacca ggtcgcccag gccttcctgt
      961 tttgcggtcc ccggggtgta ggcaaaacga cctgcgcccg tatcctggcc aaaaccatca
     1021 actgtcagaa ccttagccca aacctggaac catgcaacga atgcgacttc tgcaaagcat
     1081 tcaatcaatc ggcttctttc aatatcaacg aactcgacgc cgcttcgaac aacagcgtgg
     1141 acgacatccg gaacctggtc gaccaggtgc gcataccgcc gcagatggga aaatataagg
     1201 tatacatcat cgatgaagtt catatgctgt cgcaggctgc tttcaacgcc tttctgaaaa
     1261 cccttgaaga gcctccggca tacgctaagt tcatccttgc taccaccgaa aagcacaaga
     1321 tcatccctac catcctttcg cgctgtcaga tatttgattt caagcgcatt acagtggatg
     1381 atatcgcaaa acaccttgca tgggtcgctc aaagcgaaaa cgtttcggcc gagccggaag
     1441 ccttgcatat catagctcag aaagccgatg gcgccctgag ggacgccctt tcgatgttcg
     1501 accagatggt gagcctgggg aatcacagca ttacctacaa actggtgatc gacaacctga
     1561 atgtgcttga ttatgaatat tatttcagga tgacggatta catgcttgcc ggcgatatac
     1621 agcagtcgct cctgttgttt aatgatatta ttgacaaagg atttgaagga cagcatttca
     1681 tcaccggcct cggcgatcat ttccgcaacc tgctgatgtg ccgcgacgcc tccaccgcca
     1741 agcttatgga aaccagcgaa aacatccgca accggtatct gagccaaacc aaacaatgtc
     1801 ccatccagtt tattatcagc gcccttgaaa tcaataacaa gagcgacgtc aattaccgca
     1861 cttcgaacaa taaaaggttg ctggcagagg tcatgctgat ccagttatgc cagcttaccg
     1921 gagctaatcg cagttctcag tccggtgctg caaccgtgcc cgccgcagcc gcgccgccat
     1981 cgccggagcc tgttaaacct gccgaacctg aaaaagcaaa aacaccggtt tatcctgcgt
     2041 cgcccgcaac cgaacccgcc aaagcggaag aaagtccggc acaggcgaca cctaccgggg
     2101 aaaagccgca acccgctgaa gacaatcccg cgccgccttc tgctcttccc gacgaggaac
     2161 tgcatacagt tacgtcaggc aagccttccg gaacgcgcac cgtaatgccc gtatccttat
     2221 ccattaaatc ggctatcagc caggccaatc aaaatgcagc caaacccgaa gccgaccatc
     2281 accggacttc gtcattcacc gattcgcagc ttgccgaatc atggaacacc tttgccgccg
     2341 gaataaaaga agaatcgacg ctgttgtatt cggctcttac cggccacctg cccgtcatag
     2401 gcgaaggaaa tgtgctgaac ctggaactcg aaaacagggt actcgaagcc gagttcaacc
     2461 ggaacaagac ggatttgatc acctacctta aaaattcact caagaacgac tttatctcca
     2521 tcgaaacctc cgtttccgaa caggtaacag aggtaaagaa gccttattcc gagaaggaaa
     2581 aatttgaaca aatggctgaa aaaaatccct tgattaaaac gctgagagac cagttggacc
     2641 tggggcttga cggataaaat cttacaggca acagtctgga ctatgcgacg cgttttcaca
     2701 ctcggcgaaa cggtactcga catcctcttc aggagcggcg cccctgttaa ttctgttgca
     2761 ggcggcagca tgctgaatac ggcagttgca ctgggaagaa tgggctgtca ggtggaattc
     2821 atcagtgaat tcgggcagga tgctcccgga aaaataattg aaaattcgct tgagaaaaac
     2881 catgtttcat caaagcactc catccgctac gacgaccgtt tcaacacctc tgtcgctttg
     2941 gccttcctgg atgaaagcca aaacgcatcc tacacatttt atcatcatca accggatgaa
     3001 ttaacccgga caactgttcc cgaaacaggc cccggcgata tcctggcttt cggatcgttt
     3061 tattcggtga aacccgcccg gcgcaggcat gttgcaaaga ttgtcaggtc agcaaatgaa
     3121 tcaggtgcat tcatcatctt cgatcccaac attcgaaaaa accaccttgc tgatttaccc
     3181 tcgatacgtg aggtttttat tgaaaacatg agcctttccc atcttgtgaa aggctcgtgc
     3241 gaagatttcg catttctgac cggcgctgac aatccagcaa acatctatga ttccctgaaa
     3301 tcatactgcc cgtatattat cattacccag ggagaaaaag aagtgctttt gttcacgccc
     3361 cggttcagaa aatcatatcc tgttgaaata atcagcccga tatcgaccat aggggccggg
     3421 gacaatttca catcaggatt gatatacggt ctgctgcaat ttgagcaacc ggaatcatcc
     3481 gccggaaacc tgacgctgcc ccagtgggac aaattaatcg cttacggaat cgccttttct
     3541 gcagccacct gcctctcgtt ggagaattat atccctgaag gcttccggat caatctaaaa
     3601 atgcaatgaa aaaaaaattc gctctttcct tacttaaact tttcggatgg aaaacgagcg
     3661 gcaacctgcc gcctgacatg aagaaatgcg ttgtcgttgt agcccctcac accagcaact
     3721 gggatttttt tatcggtttt ttcggttata tggctatcgg ggtcaaggca aggtatctga
     3781 tcaagaaaga ggcgtttttc tttccattgg ggccaatcgc cagggccttg ggaggaattc
     3841 cggttgaccg cacgcggagc agcaatgtag tgccacaggt tgttgaatta ttccggagag
     3901 aagaaaacct cgtattgaca attacaccgg aaggtacccg aagccttgtc cgtcactgga
     3961 aacggggctt ttaccttatg gccgaacacg ccggcgtccc gctcgttttt ggcatcctgg
     4021 attacgggaa caaaacagtg gggttggggc ctgtgtacta ccctaccggg aattatgatc
     4081 atgatataaa ggacattgaa gcttattaca aggataccgt ggccaggcat cctgaaaatt
     4141 tcaatctcag cccgcaaaac agggaaaaat caaaatgatc cggttgcttt gctacatttg
     4201 caactgcaaa cccaggaaca ccataaaaac tctttcaaac gaccaaaagc cattaacctt
     4261 attttagcat gagtgacatt aaaattgccc tgatcgggct cggatatgta ggattaccct
     4321 tggcggtaga attcggaaaa atacttcctg ttaaaggttt cgacatcaat gaatcaagga
     4381 taaaggagct tgaaaaaggc catgaccgca cccttgagac atcggatgaa gaccttgctt
     4441 ccgccactca actcacctat acccataacc tggacgacat ccgtaactgt aattacttca
     4501 tcgtcaccgt tcctacgccc atcgataagt ataaaaggcc cgaccttacc cccctcgtga
     4561 aagcctctga aacagtaggc aaggtgctga agcaaggcga tatcgtcatt tatgaatcta
     4621 ctgtatatcc cggcgctacc gaagaagaat gcgtgccggt gcttgaaaaa ttcagcgggc
     4681 tgaaattcaa taccgatttt tactgcggtt actctcctga acgtatcaat ccgggcgaca
     4741 aggaacataa tgtaacaaaa atcaggaaag taacctccgg ttccacgcct gaagtagccc
     4801 gcaaggtaga cgatctgtac cggaaaatta ttgttgccgg cactcacatg gcatcgagca
     4861 tcaaggtagc cgaagccgcc aaggtaattg aaaattcgca gcgcgacatt aacatcgcct
     4921 ttgtgaacga gctggcattg attttcaata aactgaacat tgatacgcat gaagtgctgc
     4981 aggctgccgg caccaagtgg aattttctcc acttctcgcc aggcctggta ggcggccact
     5041 gcatcggcgt cgatccatac taccttacgc acaaggcgca ggaattgggg tataaccccg
     5101 agatcatcct agccggccgg cggcttaacg acgatatggg cttttactcg gccaaccgca
     5161 ccatcaaact catgatccat aaaggacata ccattgccgg aagcagcgta cttgtgctgg
     5221 ggatcacctt taaggaaaac tgtcccgaca tccgcaacag ccgcgtgatc gacgtgatca
     5281 atgaactaaa ggacttcggc tgtaacgtgg atgtttacga tccctgggcc gatccgcacg
     5341 aagtaaaaca tgaatacggc gttgagatgc ttccttcact tgacgggcac cggccgtatg
     5401 atgccattgt atttgccgtg gcccaccggg aattccaaca acttgacctg aagaaattta
     5461 aaaaagaaaa caccgtactg ttcgatatta agggcaccat tgacagggac cttgttgacg
     5521 gcaggctctg ataaactgac cagcataaaa caaaatgatg caacttctta ctcccaggca
     5581 ctggcgtgat tatgaactga tcgattcggg cgggttggaa aaaatcgagc gtttcgggaa
     5641 atttatcctg attcgtcccg agccccaggc cctgtgggat cgttcgatgt cgcgggaaga
     5701 gtggcagggc ctgtcacatg cagaattcag gaaagagaag aatgatcccg aaaagggaaa
     5761 atggatcctg aaaagaggca cgcctgagca atgggaaatc ggttacgcca acggtgaaat
     5821 gaaactgcgg atgcgactgg gtcttactgc tttccggcat ataggcgttt tccccgagca
     5881 ggccgagaac tgggatttta tcttcaatac catcggttcg ttcccgaagc cttcacccag
     5941 ggtgttgaat cttttcgcct atacaggcgg cgcatcgctg gcggcaaggg ccgccggcgc
     6001 cgacgtgctg caccttgatt cggtgaagcc ggtggttacc tgggcgcgcg aaaacatgga
     6061 actcagccgt cttgatggca tccgctggat tgttgaagac gcgctgaagt ttgttcagcg
     6121 tgaagtgcgt cgcggcagca aatactcggg catcattctc gatcccccgg cttatggccg
     6181 cggaccggaa ggtgaaaaat ggataatcga cgatggttta cccgagatga tcaaacactg
     6241 caaccagttg ctggagcctg aaaactcctt tttaatcctc aatatgtact ccatcgggtt
     6301 ctcagcactg gtcgccgaca atctgatcaa ttcctgtttc agcaaaaccg ggaataaaga
     6361 atacggcgaa ctatatctca gcgataaatt ctcaaagagg ctgcctctgg gaactttcct
     6421 gcggtttgtc agatagaatc cccgaaaggc agtttagata taaaacacct ccacttcgcc
     6481 gaatgaaacc cgcacatcga ttgtaaaacc aggctgcgcc gggttataat caggcgacat
     6541 ataggtatat tccccgaaag atgctgtttt catatccggc ccccgcaggg tgccgaacga
     6601 tacgtttccc ttaactttca aaggcacttc acggtcggcg atgatcctga actgcccgaa
     6661 tgaacactcc accgacagcg ggatatttga atcaggcttt tgtcctttca ggttcaggct
     6721 gccttcacca aaaagtacat tgtaatgttg ctgcccgggc gtaaaggcaa attttgtatc
     6781 gccaaactgt acatgctgag cgtcgtgttt tggcgcgaaa cttcctctta tcataaaaac
     6841 gccccctacg atcagaaccg cggaaagtaa aatcgtaaag acaggaatat caaggtcaaa
     6901 aatattgcgg accagaaaga aaacccctat cacaacaatg attatgccta tagccgaagc
     6961 tccggaaaaa agcttgttca tggtttgatt tttaagcgtt gaacaaatat aacataattt
     7021 cattaatttc ctgaaaatcc aggcggtcct gaaaaggcgt ggattatcgg ggcatagccg
     7081 ttgtttctgg gtctgtggct ttttttatat tgtggtt
//
LOCUS       DAOPYV010000108         6695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_13943_length_6695_cov_12.192922, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000108 DAOPYV010000000
VERSION     DAOPYV010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6695
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13943_length_6695_cov_12.192922"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PKI35_11035"
     CDS             <1..444
                     /locus_tag="PKI35_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44960.1"
                     /translation="DAEGGFFDRGQPYVNLYNSLGSYRPFGLWSVNSFATMRMGEFSA
                     DKYAAVFISHNFGKLLFRSKHFNPEPVLVTNAGFGSLRNAPQHNEKDVKDFHYGYYES
                     GLVLNNIVSMGFAGVGVGAFYRYGPYSLPAIKDNLALKITVSVSF"
     gene            553..2559
                     /locus_tag="PKI35_11040"
     CDS             553..2559
                     /locus_tag="PKI35_11040"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNX44961.1"
                     /translation="MNTPEFRQAISQGIPSELPPPQPYDQAVNHAPVRKDILTPAEKT
                     LAVRNALRYFDKKHHAVLAPEFAEELKKYGRIYMYRFRPSYPMFARPVSEYPAKCQQA
                     AAIMLMIQNNLDPAVAQHPHELITYGGNGAVFQNWAQYLLTMKYLSEMTDEQTLVMYS
                     GLPLGLFPSHKDAPRVVVTNGMVIPNYSKPDHWEKFNALGVSQYGQMTAGSYMYIGPQ
                     GIVHGTTITVMNAGRKISSNNESLAGKLFVSSGLGGMSGAQPKAAVIAGAIGLIAEIN
                     PKAVKTRFSQGWVEEVYDNLDQVIARVRGAKAGKESVSIAYQGNIVDLWERLAAEDIR
                     VELGSDQTSLHNPWAGGYYPVGLTLEESQKLMAEDPVKFREKVQESLRRQIAAINKLT
                     ARGMYFFDYGNAFLLESSRAGADVMNENGTFRYPSYVQDIMGPMCFDYGFGPFRWVCA
                     SGDPADLDTTDHLAIDVLEKLASESPAEIMQQMHDNIQWIREAKQNKLVVGSQARILY
                     ADARGRISIAAAFNDAIKKGLLKGPVVLGRDHHDVSGTDSPYRETSNIYDGSSFTADM
                     AIQNVIGDSFRGATWVSIHNGGGVGWGEVINGGFGMLLDGSEDAGRRLRNMLFWDVNN
                     GIARRSWARNDGAMFAVQRAMKDEPRMKVTFPNLVDDSILEGLF"
     gene            2866..3528
                     /locus_tag="PKI35_11045"
     CDS             2866..3528
                     /locus_tag="PKI35_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44962.1"
                     /translation="MKRALVQFFLLLLAPSAFARIAGTQAENIDMARLDAGEVISMIG
                     STRDSTGQFSLSTDSLSKYYLLIKHDKRDRQRIIGEGRRIKIWIDTACYMGIISGIVA
                     DSILLDGIWYDIASIDRLRIRTAGTNITGTLLTSGGAITTPLGIVLLAEGISMMTSRG
                     GNDFGVFFQNLFGMIMIATSVVIIPVGAILLGSGIFIILHGKQFNFNKHWRITTEKGV
                     IR"
     gene            complement(3592..5283)
                     /locus_tag="PKI35_11050"
     CDS             complement(3592..5283)
                     /locus_tag="PKI35_11050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44963.1"
                     /translation="MKKTLLSVLVTLLTVITFGQSQRFILFEEFTNACCGPCASQNPA
                     FDALLTTNAGKCTSIKYHTNWPGPDPMNAQNPTEVATRVTYYGVSGVPHAVMDGSPLT
                     GSSYSGAPANLTQTKIDNEYAVPSPFELFVNQHLSPNNDTIFVTVLGKATQEVTGTLV
                     AHIAVIEKHIHFNSAPGSNGEKDFYNVMKKMLPKASGTTLPNHFFTGDYFIIEQAWAL
                     ENVYDINELNVVGFIQNNQNKNVLQAANTSADPINGVYENDIELMSLSNLTPTYCTNS
                     LAPVVNIRNNGSLPLNSVTFQYKVNDGMYQSYTWTGALNTLEKAAVQLPALDYTLKEN
                     NELTVFASGINGGNDNYPKNDTIHYSFTDALQVGTTATVKIKTDNNPQETTWTITDAS
                     GAIIASGGPYTNAATTYTQDVNLDFGTCYQFTIYDAGGNGICCKNGNGYYKVTSGSTT
                     IESGTQFGSSESAQFYSQSGVGIPSLSSVIKTSLFPNPVKNLATLSFENDRTENVSIN
                     VFDILGNTVIRIPATQYPAGLHQATLDCGNLSNGLYNVRLCTGSRSINLKMTVNK"
     gene            5533..6657
                     /locus_tag="PKI35_11055"
     CDS             5533..6657
                     /locus_tag="PKI35_11055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44964.1"
                     /translation="MKKLLLSVVLISGFLFASAQEMEILFQNTVVNNETITVYGDTTF
                     NTIECLLKVRNNTSADLTNVYVRRITVAEVPGSINSFCFGICYPPFVDTSVMATSIAA
                     GATDSSFVGDYYPEKLNGVTSITYEFFDNITLANRVAAQVTVNYLTSFISFPIIDGNT
                     TEANNSTLVVFTSDNQTEYLEKKLKLVNNSPSRVEMYVRRIINSEVDGSENSFCFGSM
                     CYPSFTDTSSLVNIIAAGAVDSSFKADYYPNGHPGPTSITYEFFTKTKGREDIRESVT
                     VIFKMSGVGINENAMAIQRLYPNPAPAFVAVEYEIKPESGDVWAVIRNLSGQVAKRAL
                     IDSNSGKAVIDVTGLADGVYTFSLMEGNKIVSTRKLIVQH"
BASE COUNT         1744 a         1491 c         1630 g         1830 t
ORIGIN      
        1 gatgccgaag gaggcttttt tgaccggggc cagccatacg taaatcttta caattcactg
       61 ggaagttaca gaccttttgg cttgtggtcg gtcaacagtt tcgcaaccat gcgcatgggt
      121 gagttttctg ccgacaagta tgccgcagtt tttatatcgc ataatttcgg gaaactgctt
      181 ttccgttcca aacattttaa ccctgaaccc gttcttgtca cgaatgccgg tttcggtagc
      241 ctcaggaatg ccccgcagca caatgaaaag gacgtcaaag actttcatta cggctattat
      301 gagtcgggat tggtgctgaa caacatcgtc agcatggggt ttgccggtgt tggcgtcgga
      361 gctttctatc ggtacgggcc ttattcatta ccggcaatta aggacaacct ggcgctgaag
      421 atcaccgttt cggtttcatt ttaattttac ggcagtacga tttcccacta tttcctgaaa
      481 gcttaagcca aaaggattta ttagctttgt gcattcttct gcaggatgca aacgagattt
      541 aattaactga aaatgaatac accagagttt cgccaggcta tttcacaggg aataccttct
      601 gaattgccgc ccccccaacc atacgatcag gctgttaacc atgcgccggt acgcaaggat
      661 atacttacac ctgccgaaaa gacactggcg gtgaggaatg cattgcggta tttcgataaa
      721 aagcaccatg ccgtgcttgc cccggaattt gccgaagaat tgaaaaagta cggccgtatt
      781 tacatgtacc gcttcaggcc atcctatcct atgtttgcca ggccggtcag tgagtatccg
      841 gcaaaatgcc aacaggcggc tgctattatg ctgatgatcc agaacaacct ggacccagcc
      901 gtagcgcaac atccgcacga actcattacc tatggcggga acggcgctgt tttccagaac
      961 tgggcgcaat acctgctaac catgaaatac ctgtcggaaa tgaccgacga acaaacgctt
     1021 gttatgtatt ccgggcttcc cctgggactg ttcccttcgc ataaagacgc tccccgggtg
     1081 gtagtcacca atgggatggt aatccccaac tattcaaaac ccgaccattg ggaaaagttt
     1141 aatgcgctgg gagtttccca atatggacag atgacggcag gctcctatat gtacatcggc
     1201 ccgcagggaa ttgtgcatgg taccaccatc actgtgatga atgccgggag gaaaatctca
     1261 agtaataatg aaagcctggc cggcaaactc tttgtatcat caggtcttgg cggaatgtcg
     1321 ggcgctcagc caaaagccgc cgtaattgcc ggtgcgattg ggctgattgc cgagattaat
     1381 ccaaaagccg tgaaaacacg tttttcacag ggctgggtgg aagaagttta tgacaacctc
     1441 gaccaggtga ttgcccgtgt aaggggtgca aaagcaggaa aagaatccgt ttccattgcg
     1501 taccagggaa atattgtaga tctgtgggag aggctcgccg ctgaagatat cagggtggaa
     1561 ctgggttctg accagacttc cttacacaat ccctgggcag gcgggtacta tcccgttggg
     1621 cttacactcg aagaatcaca gaaacttatg gctgaagatc cggtgaagtt ccgcgaaaaa
     1681 gtacaggaat cattgcgcag gcagatcgcc gccatcaaca aactgacggc cagggggatg
     1741 tatttctttg attatggtaa tgctttcctt cttgaatcat cccgcgccgg agccgatgtt
     1801 atgaacgaaa acgggacatt ccgctacccg tcgtatgtac aggatattat ggggcccatg
     1861 tgttttgatt acggcttcgg tccgttccgc tgggtatgcg cttcgggcga tcccgccgac
     1921 ctcgatacaa ccgatcatct ggcgatagac gtactggaaa aactggcgtc tgaatcgccg
     1981 gcggaaatta tgcagcagat gcacgacaac attcaatgga taagggaagc caaacaaaat
     2041 aaactggtcg tgggttccca ggcgcgaatc ctttatgccg atgcccgcgg acgaatcagc
     2101 atagctgctg ctttcaacga tgccatcaaa aaagggctat taaaaggccc ggttgtgctg
     2161 ggacgcgatc atcacgatgt ttcgggaacc gactcgccat acagggaaac ttcaaatatt
     2221 tatgacggtt cttctttcac tgccgacatg gctattcaga atgttatcgg cgatagtttc
     2281 cgcggcgcaa cctgggtttc gatccacaat ggcggcggcg taggctgggg cgaagtgatc
     2341 aacggcggat tcgggatgct tcttgatggc agcgaagatg ccggccgccg gttaaggaac
     2401 atgcttttct gggatgtgaa caacggaatc gcacgccgga gctgggcccg taacgacggc
     2461 gccatgtttg ccgtgcaacg ggcaatgaag gatgaacccc gcatgaaggt taccttccct
     2521 aacctggtag atgacagtat cctggaagga ttgttctaag ctttttcttt cgtggtattc
     2581 ataaattggt ggtcaaatcg tgatcatcag gcagaatatt atccgtgagc gggaatcatt
     2641 tctgttttat ttggaatatt ttctgaaatt ttgtaattta acaccttgca gcccgccggt
     2701 attttattgg cgttttcagg tatggagcta ttttctatag tacactgaca gtatgacctg
     2761 agtgccgttt ggctgccggc ctgtgtttta cgacccatga tattacttgt ttgccttgta
     2821 tccttgtcac gaaaaggcaa tagttaacag gtaaaaatta tcatcatgaa aagggccctt
     2881 gttcagtttt ttctccttct gttggcgcca tcggcttttg cccggatagc cggaacacag
     2941 gcagaaaaca ttgatatggc ccggctggat gccggtgaag taatctccat gattggatca
     3001 acccgggatt caaccggaca attttcgctg tcgaccgaca gcctgtcgaa atactacctt
     3061 ctgataaagc atgataaaag agacagacaa aggataatcg gagaggggag aagaataaaa
     3121 atatggattg atacagcctg ctatatggga ataatttccg gtattgttgc cgatagtatc
     3181 ctgcttgatg gcatctggta tgatattgct tccattgacc ggctcaggat acgtactgcc
     3241 ggtacaaata ttacaggaac tttgctgacc tccgggggcg ctatcacaac cccgctgggg
     3301 atcgtgttac tggcagaagg gataagcatg atgacttctc gcggaggtaa tgatttcggc
     3361 gttttctttc agaatttgtt tggtatgata atgatcgcga caagtgtggt cattattcct
     3421 gtaggcgcaa ttttgctcgg cagcgggatt ttcattattc tccatggcaa acaatttaac
     3481 tttaataaac actggagaat cacaacagaa aaaggtgtga taagatagct taccacacct
     3541 tcaattggag tttatcagaa cttttcaggc tgagaagcgc tggctgatgt attacttgtt
     3601 tacagtcatc ttcaggttta tcgacctgct gccggtgcaa agccttacgt tgtaaaggcc
     3661 gttgctgaga tttccgcaat cgagggttgc ctgatgcagc cctgcaggat attgagttgc
     3721 aggaatccgg attaccgtat ttccgagtat atcaaaaaca tttatgctga cgttctccgt
     3781 ccggtcattt tcaaaggaca acgttgccag gttcttgaca ggatttggga atagcgatgt
     3841 tttaataacc gatgaaagcg aaggaatacc tacccctgat tgcgaataga attgtgcact
     3901 ttcggacgat ccgaactgtg taccggattc aatcgtagtg cttccactgg ttaccttgta
     3961 atatccgttt ccattcttgc aacagatgcc gttgcctcct gcatcgtata tggtgaattg
     4021 atagcaggtg ccaaaatcaa ggttgacgtc ctgtgtatag gtggtggcgg cattggtata
     4081 aggcccgccc gaagcaatga tggctccgga agcatcggta attgtccagg tggtttcctg
     4141 agggttatta tcagttttga tttttacggt tgccgttgtt ccaacctgca acgcatcggt
     4201 aaaggaatag tgtatggtat cattcttcgg gtaattgtcg ttgccgccat tgatgccgga
     4261 ggcaaaaaca gtaagctcgt tattttcctt taaagtataa tcgagagccg gcaactgcac
     4321 ggcagctttt tcaagcgtat tgagagcgcc ggtccaggta taggactgat acatgccatc
     4381 gttcacctta tattggaatg taacgctgtt tagcggcaaa gatccgttat tacggatatt
     4441 aacaaccggg gcaaggctgt ttgtacaata ggtaggcgtg agatttgaaa ggctcatcaa
     4501 ttcaatgtcg ttttcataaa cgccgttgat gggatcggct gaagtattgg ctgcctgcag
     4561 aacattcttg ttctggttgt tctgtatgaa cccgaccacg ttcagttcat tgatgtcata
     4621 aacgttttcg agagcccagg cttgttcgat aataaaataa tcaccggtga aaaaatgatt
     4681 cgggagtgtg gtcccggatg ctttcggcaa catctttttc atcacattgt aaaagtcttt
     4741 ttcgccgttt gagccggggg ctgaattgaa gtggatatgt ttctcgatga cagctatgtg
     4801 cgccaccagg gtgccggtta cttcctgagt ggctttgcca agcacggtta caaatattgt
     4861 atcgttgtta ggcgacaaat gctgattaac aaataattcg aatggcgaag gaacagcata
     4921 ttcgttgtct attttcgttt gagtgagatt agcgggggcc ccggagtaac tgcttccggt
     4981 cagaggtgat ccgtccataa cagcatgagg cacgcccgat acgccgtaat atgtaacgcg
     5041 ggtggccacc tcggtaggat tttgcgcatt catcggatcg ggacccggcc agttggtatg
     5101 gtattttatt gaagtgcact taccagcatt tgttgtaagt aaggcatcaa aagcggggtt
     5161 ttgcgatgcg catggcccgc aacatgcatt tgtgaactct tcgaagagaa taaacctttg
     5221 tgattggccg aacgtaatta ccgtcaaaag agtaacgagt accgatagta acgttttttt
     5281 catgttttta tggattggtt tgcagtagtt tgagctgtat attgtgacaa agatcgtaat
     5341 taatctgtaa ataaacgctg tactaaacta tctacataca ttctttatta aatattttct
     5401 aaaggcagga aatgtgttgc caaaaagcat tacttttgaa gcaaactgta acaacaccgg
     5461 tgggctggca acgattcagg ccacaggatc aatgaatact catcaactca catcctgtca
     5521 aaaaaaacga ttatgaagaa acttttactc tccgttgtac tgatttcggg attcctgttt
     5581 gcctcagcgc aggaaatgga aattctattt caaaatacgg tagtcaacaa tgaaactatc
     5641 acggtttatg gcgatacaac cttcaatacg atcgaatgcc tcctgaaagt aaggaataat
     5701 acctctgccg atctgaccaa tgtgtatgtc cgccgtatca ccgtagcaga ggttccgggt
     5761 tcaataaatt cattttgctt tggcatctgc tatccgccat tcgttgatac ctcagtaatg
     5821 gctacctcca ttgccgccgg cgccacggat tcgtcattcg tcggagatta ctaccctgag
     5881 aaacttaacg gcgtcacttc cattacgtat gaatttttcg ataatataac attggcgaac
     5941 cgggttgccg cgcaggttac cgtgaattat ttgacatcct tcatttcttt cccgatcatc
     6001 gacggtaata ccactgaagc caacaattca acgctggtgg tatttacttc agataatcag
     6061 acagaatatc tggaaaagaa actgaaattg gtcaataatt ccccatcccg ggtcgaaatg
     6121 tatgttcggc gcattatcaa cagtgaagta gatggatcgg aaaactcgtt ctgttttggt
     6181 tccatgtgct atccttcctt taccgatacc tcttctctgg tcaatatcat tgctgccggg
     6241 gctgtcgatt cctctttcaa agctgattat tatcctaacg gacatcccgg tccgacatcg
     6301 attacctacg aatttttcac caaaaccaaa ggccgtgaag atatacgtga atccgtgaca
     6361 gtgattttca aaatgagcgg agtcggtatc aacgaaaatg caatggccat acaacgcctt
     6421 tatcccaatc cagcccctgc ctttgttgcc gttgaatacg aaattaaacc cgaaagcggg
     6481 gatgtatggg cagttatccg gaatttatcg ggtcaggtgg caaagagggc gcttatcgac
     6541 agcaactccg gcaaagccgt gattgatgtt accgggcttg cagatggcgt ttataccttc
     6601 tctttgatgg aaggcaataa aattgtatcc accaggaaac tgattgttca gcactaatcc
     6661 tttaaaagcc ttttaaaggc ttcacaacag aaaaa
//
LOCUS       DAOPYV010000109         6079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_16077_length_6079_cov_12.686255, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000109 DAOPYV010000000
VERSION     DAOPYV010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16077_length_6079_cov_12.686255"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PKI35_11060"
     CDS             complement(<1..596)
                     /locus_tag="PKI35_11060"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HNX44965.1"
                     /translation="MLKKSFLIVFLVLLADQALKVWIKTTMTLGQEISMAGDWAILHF
                     TENNGMAFGMQFAGDYGKLALSLFRIVAVIFLGWYIWYLAKRGTKFGVIACFSLIMAG
                     AMGNIIDSAFYGMIFSESTFFDVAGMFPAGGGYSSFLHGKVVDMLYFPVLQGTYPQWF
                     PFLGGQEFLFFRPVFNISDSAITTGVLSLIIFERKFFQG"
     gene            complement(619..1020)
                     /locus_tag="PKI35_11065"
     CDS             complement(619..1020)
                     /locus_tag="PKI35_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNX44966.1"
                     /translation="MKADQKKEETTKTRYSDEELEEFRLIILEKLEKARRDLQLLTEA
                     YTNSNDNDTMDTSPTFKVLEEGYQVMSKEENSRLATRQEKFIQNLENALLRIENKTYG
                     ICRVTGKLISKERLRSVPHATLSIEAKLSQK"
     gene            complement(1073..4447)
                     /gene="ileS"
                     /locus_tag="PKI35_11070"
     CDS             complement(1073..4447)
                     /gene="ileS"
                     /locus_tag="PKI35_11070"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNX44967.1"
                     /translation="MQNNYPEYKQLNLTRIGSEVLDFWKQNNIFHKSLEYRADHQPFI
                     FYEGPPSANGQPGIHHVMARAIKDIFCRYKTLKGFHVARKAGWDTHGLPIELAVEKTL
                     GITKEDIGRTISVDEYNKACRNEVMKYKDMWDDLTEKVGYWVDLDQPYITFENTYIET
                     CWYLLAQLYRKGLLYKGYTIQPYSPAAGTGLSSHELNQPGCYRDVKDNTVVAQFKVRN
                     DADHPLITDLFGNDGREVFILAWTTTPWTLPSNTALAVGAGIEYVLVETFNPYTYLPV
                     KLVLAKPLLNRYFPEANAVLSFEEYQPGQKQIPFKVLKEFTGARLAGLRYEQLLPYAQ
                     PETGDAFVVVTGDFVTTEDGTGIVHIAPSFGADDFKVARQHGIGALTMVNKDGRFNDQ
                     MGEFAGRFVKPEYSPDFESGTVANVDIDIIVKLKKENRAFKAEKYEHSYPHCWRTDKP
                     ILYYPLDSWFIRTTALKDRLIELNRTINWKPEATGSGRFGNWLENLVDWNLSRSRYWG
                     TPLPIWVSEDRSEEMIIESVAQLKAEIEKSMAAGFMKENPLKNFKEGDTSHENYTSFD
                     LHRPYVDDIVLVSSTGKKMLREPDLIDVWFDSGSMPYAQIHYPFEHKDGLNEYFPADF
                     IAEGVDQTRGWFYTLHAIATLAFDSVAFKTVVSNGLVLDKDGNKMSKRLGNAVDPFET
                     IKKYGPDALRWYMVTNSQPWDNLKFDADGITEVQRKFFGTLYNTYSFFALYANIDGFR
                     YAEADVAPDERPEIDRWILSELNTLIRNVDESYEQYEPTRAGRLIADFVNDHLSNWYV
                     RLSRRRFWKGEYSTDKISAYQTLYHCLATVAQLASPIAPFFTDRLFIDLNKVTGKVKA
                     ESVHLTDFPVADASMIDKPLEERMELAQQFSSMVLSLRKKTSLRVRQPLQKILIPAAS
                     EEFKAQIKRVSNLILSEVNVKELEFLAEDAGFLVKRIKPNFKTLGPRYGKLMKEIAAA
                     ISAFTREQINSIEKNGRYAIEINGQPVEVELADVEIATEDIPGWVVANQGALTVALDI
                     TLTPSLQEEGIARELVNRIQTLRKDKGFEVTDKITLKVEKNPLTESAIANNLLYICSE
                     TLAEKLTTENLDNNPDKDTVELTDEITISIVVTKIT"
     gene            4807..>6079
                     /locus_tag="PKI35_11075"
     CDS             4807..>6079
                     /locus_tag="PKI35_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44968.1"
                     /translation="MQHSEAISPKVNARAGGICDFDHFIMRILFFFLCIIVLHINTVA
                     QEWIRYYGQGQNAIAWNVTEQYDKGYLIAGSINLSTYSWLVKTDVNGNVLWDKKFGNG
                     NYITVLMNVEQTTDGGFILCGSTMKLSSNDAFILKLNPCAEVEWCKILEGPNNFENGI
                     KVKQSPDGGYIVLTAYYPTNPESNISLFKFNPAGDLVWHQFYPHANYYNDDQPCDLLT
                     DGDGNLITASRYAPDSGTNSPQVIRSYFVKTDTAGNKIWDLVYGTNKHYYSDVWETTK
                     NSNGKYYHSCTHSATNGTNPGLIWISHNGSESGDKDIFETGSPNFCGLSTITMLHDTN
                     LVFFGGFTINGTFQWQALKTDTLGNIINSSSISVVILYCSATRTFDDKFVTVGVDTYN
                     NKYRIVALKVNSNLEYDSLYTQPFTYDSLCPH"
BASE COUNT         1503 a         1392 c         1525 g         1659 t
ORIGIN      
        1 ccctggaaaa atttccgctc gaagatgatc aggctcagca caccggtcgt gatggcgctg
       61 tcggagatgt tgaaaacggg acggaaaaac aggaattcct ggccgcccag gaatgggaac
      121 cattgcggat aagtaccctg taaaaccggg aaataaagca tatcgacgac tttaccgtgc
      181 aggaatgagc tatagccgcc cccggcagga aacattcctg caacatcaaa aaaggtgctc
      241 tcggaaaaaa tcatcccgta aaaggcgctg tcgatgatgt ttcccatggc gcctgccatg
      301 atgagcgaga aacaggcaat cacgccgaat ttggtcccgc gtttggccag ataccagata
      361 taccatccca ggaaaattac ggcaacgatc cggaataaac ttaaggccag cttgccgtaa
      421 tcgccggcaa actgcatacc gaatgccata ccgttatttt cggtaaaatg aagtatggcc
      481 cagtcgcctg ccattgaaat ttcctgacca agggtcatgg tggtttttat ccacaccttg
      541 agcgcctggt cggcaagcaa cacaagaaaa acaattaaaa acgatttttt aagcatcctg
      601 ctctttcaaa tttttaactt acttctggct cagcttggct tcgatgctta aggtagcatg
      661 aggaacgcta cgcaaccgtt ctttggaaat aagcttcccg gtgaccctgc aaatgccata
      721 cgtcttgttt tcgatacgca gcagggcgtt ttccaggttt tgtataaatt tttcctgcct
      781 tgtggcaagc cgcgaatttt cttctttcga cattacctgg tatccttctt caagtacctt
      841 gaaggtcggg gacgtatcca tagtgtcgtt atcgttactg ttggtatatg cttcggtaag
      901 cagttgcaga tcacgacggg ctttttcgag tttctccaat atgatcagcc ggaactcctc
      961 aagttcttca tcggaatacc tggttttggt cgtttcctct ttcttctggt ccgctttcat
     1021 gattcctaag gtattagtag tacggttaat aatttgcccc ggttatatcc cgtcaggtta
     1081 ttttggttac cacgatactg atcgtgatct catccgtaag ttcaacggtg tccttgtcgg
     1141 gattattgtc caggttttcg gtggttaatt tttcagctag ggtctcggaa caaatatata
     1201 gtaaattgtt cgctattgca gattcggtca aaggattttt ttcaaccttc agcgtgatct
     1261 tatcagtaac ttcaaatcct ttgtccttac gaagggtctg gatgcggttg acaagttcgc
     1321 gggcgatgcc ttcttcctgc agcgaaggtg tcagggtgat atcgagggcc acggtaagcg
     1381 ctccctggtt cgccacgacc catccgggga tgtcttcggt ggctatttca acatcggcga
     1441 gctccacctc caccggctgt ccattaatct ctatcgcgta tctgccgttc ttttctatgc
     1501 tattgatctg ttcccgtgtg aaagcgctga tagcagcggc aatttctttc atcagtttgc
     1561 cgtatctggg acccagcgtt ttgaaattgg gttttatacg tttcaccagg aaaccggcat
     1621 cttcagccag aaattccagc tctttcacgt ttacttcgct caggatgagg ttgctcaccc
     1681 gttttatctg cgctttaaat tcctcgctgg cagccggtat cagtatcttc tgcaagggtt
     1741 gccgcacacg gaggctggtt ttcttgcgca ggctcagtac catggaggag aactgctgag
     1801 ccagttccat acgttcttcc agcggcttgt cgatcatgga ggcatctgca accgggaagt
     1861 cggtgagatg aaccgattcg gccttcacct tacccgttac tttgttcagg tcgatgaaaa
     1921 gcctgtccgt gaagaatggg gcaatgggcg acgccaactg ggcaaccgtg gccaggcaat
     1981 ggtataacgt ctggtatgcc gagatcttgt ccgtggaata ttctcctttc cagaaacggc
     2041 ggcgcgacag gcgcacatac cagttgctga ggtggtcgtt tacgaaatcg gcgatcagcc
     2101 ggccggcgcg tgtcggttca tattgttcgt aggattcatc cacgttcctg atgagcgtgt
     2161 tcagctccga aagtatccag cggtcaattt ccggacgttc atccggagca acgtcggctt
     2221 cggcatacct gaaaccgtcg atattggcat ataaggcaaa gaagctgtat gtattgtaca
     2281 gtgtaccgaa gaatttacgc tgcacttcgg taatgccgtc ggcgtcaaac ttcaggttgt
     2341 cccagggctg cgaattggtg accatgtacc agcgcaacgc atcgggcccg tattttttga
     2401 tggtttcaaa aggatcgacg gcattgccga ggcgtttcga catcttgttg ccgtccttgt
     2461 caagcaccag cccgttggaa actacggttt tgaaagctac cgaatcgaaa gccagcgttg
     2521 caatggcatg gagggtgtaa aaccagccgc gcgtctggtc aacgccttcg gcgatgaaat
     2581 cggcggggaa atattcgttc aggccgtctt tgtgttcaaa ggggtaatga atctgcgcat
     2641 aaggcatgga gcccgagtcg aaccacacgt cgatgaggtc cggttcgcgg agcatcttct
     2701 tccctgtgga tgaaaccagc acgatgtcat ccacgtaagg ccggtgaagg tcgaaagagg
     2761 tataattttc gtgcgaagta tcgccttctt tgaaattttt aagcgggttc tctttcatga
     2821 aaccggcggc catgctcttc tcaatttcag ccttgagctg agcgaccgat tcaatgatca
     2881 tctcttcgct gcggtcttcc gatacccaga tgggcaaagg cgtgccccag taacgtgagc
     2941 gcgaaaggtt ccagtcaacc aggttttcga gccagttgcc gaaacgcccc gaaccggtgg
     3001 cttccggctt ccagttgatg gtccggttga gttcgatcag gcggtctttg agcgccgtgg
     3061 tgcggataaa ccacgaatcg agcgggtaat aaagaatggg cttgtcggta cgccagcagt
     3121 gcggatatga gtgctcgtat ttctcggctt taaaggcgcg gttctctttc ttcagcttga
     3181 cgatgatgtc gatatccaca tttgcgaccg ttcccgactc aaaatcgggg gagtattccg
     3241 gtttgacgaa gcgtccggca aattcaccca tctgatcgtt gaaacggcca tccttgttca
     3301 ccatcgtcaa agcgccgatg ccatgctgcc gggccacctt aaagtcgtcg gcgccgaagc
     3361 tgggggcaat gtgaacgatg ccggttccgt cttcggtagt gacaaaatcg ccggtcacca
     3421 ccacgaaagc gtcgcctgtt tccggctggg cgtaaggaag cagttgttcg taacgcaaac
     3481 cggccagtcg ggcgcccgta aactctttca ataccttgaa aggtatttgt ttctgacccg
     3541 gctggtattc ttcgaacgaa aggacggcgt ttgcttcagg gaaataacgg ttcagcaggg
     3601 gttttgccag caccagtttc accggaaggt aagtataggg gttgaaggtt tcaaccagga
     3661 cgtattcgat gccggcgcca acggcaaggg cggtgttcga gggcagcgtc cagggtgtgg
     3721 tcgtccaggc caggatgaag acttccctgc cgtcgttgcc gaacaggtcg gtaatcaggg
     3781 gatgatccgc gtcgttgcgg actttgaact gggcaaccac ggtgttgtct ttcacatcgc
     3841 ggtaacaacc gggctggttc agctcgtgcg acgacaggcc tgtcccggcg gccggcgaat
     3901 agggctgaat ggtgtatcct ttgtacagca ggcctttcct gtaaagttgt gccagcaggt
     3961 accagcaggt ctcaatgtag gtattttcga aagtgatgta aggctggtca aggtcgaccc
     4021 agtaaccgac tttttcggtc aggtcgtccc acatatcctt gtatttcatc acttcgttgc
     4081 ggcaggcttt gttgtattcg tccaccgaaa tggtgcggcc gatgtcttcc ttggtgatgc
     4141 cgagggtttt ttcaacggca agttcgatgg gcagtccgtg ggtatcccag ccggctttgc
     4201 gggccacatg aaaccctttg agcgttttat agcggcagaa aatatctttg atggcacggg
     4261 ccatcacgtg gtggatgccc ggctggccgt tggccgaagg cggcccttca tagaaaataa
     4321 aaggttgatg gtcagcgcgg tattcgagac ttttatggaa gatgttattc tgtttccaga
     4381 aatcgagcac ctcgctgcct atgcgggtca aattaagttg cttgtattca ggatagttgt
     4441 tttgcatccg gcagaacgtt gaacgtgttt taaaaaaggc tgcaaaggta gtaattttta
     4501 tgagaattaa caggatggaa ttaaacggat tacgacgcct ccaggccttc cggcgtccgt
     4561 ctttgcgtca ggcagggcgc aggagagaat acagggtatg ccgttgtatt aatggtccgt
     4621 gcgaaccgga ataaaatgaa aaaatatggc gctttattgt taaaattttt gtacttttcc
     4681 cacattgcta tgtgtgataa ggccggcgga ataattgttt taaggtattg ctgtttgaaa
     4741 aaggtgttta agaagcagga gtaatgaaac ggatggagac gcttattaag gattcccgcg
     4801 caggcgttgc aacatagcga agcgatctca ccaaaggtaa acgcaagagc ggggggaatt
     4861 tgtgactttg accattttat catgagaatt ctctttttct tcttatgtat tatagtacta
     4921 catatcaata ctgttgcaca ggaatggata cgatattatg gccaggggca aaacgctatt
     4981 gcctggaatg taactgaaca atacgacaaa ggctacctga tagcaggaag tataaattta
     5041 tcaacttatt cctggttagt aaagactgat gtaaatggca atgtactatg ggataaaaag
     5101 tttggtaacg gtaattatat tacagttcta atgaatgttg aacaaacaac tgatggaggt
     5161 ttcatacttt gtggctcaac aatgaaatta tcatctaatg atgcatttat tctaaaacta
     5221 aatccatgtg ctgaggttga gtggtgtaaa atattggaag ggccaaataa ttttgaaaat
     5281 ggtataaagg taaagcagtc gcctgacggg ggatatatag tattgactgc ctattatcct
     5341 accaaccccg aatccaatat ttcattattt aaatttaacc ccgcaggtga tcttgtatgg
     5401 catcaattct atcctcatgc taattattat aatgatgacc aaccatgtga tctcttgact
     5461 gatggtgatg gaaaccttat aactgcctca cgttatgccc ccgattcagg cacaaattca
     5521 cctcaggtta tcagaagcta ctttgttaaa accgatactg ccgggaataa aatatgggac
     5581 ctggtttatg gtaccaataa acattactat agcgatgttt gggaaacaac taaaaatagt
     5641 aacggtaaat attatcattc ctgcacccat tcagctacaa atggaacaaa tcccggactt
     5701 atctggatca gccataacgg gagcgagtca ggagataagg atatttttga aaccggtagt
     5761 ccaaatttct gtggactttc aaccattacg atgcttcatg atacaaacct ggttttcttt
     5821 ggtggtttta caattaatgg cactttccag tggcaagccc ttaaaactga tacattaggc
     5881 aatataataa attctagtag tatttctgta gtgatcttgt attgctccgc aacaagaacc
     5941 tttgacgata aatttgttac agttggtgtg gacacttata acaataaata tagaattgtc
     6001 gctttaaaag tcaactccaa cttagaatac gacagcctct acacccaacc ctttacctac
     6061 gacagtctgt gcccgcacc
//
LOCUS       DAOPYV010000110         6028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_16269_length_6028_cov_12.251298, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000110 DAOPYV010000000
VERSION     DAOPYV010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16269_length_6028_cov_12.251298"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PKI35_11080"
     CDS             <1..632
                     /locus_tag="PKI35_11080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44969.1"
                     /translation="SYRDEVMIDFNDQYSGEGGSYKFGSLYAEAPELWSVKNGVSYTI
                     DRYKEITSGLAVHISAKCGVTGAYTLTATNIGDFSLSNIVYLEDLKTGTKVDLKAVGS
                     YSFTGSPNDNKDRFRVTFAEIAGAEEPAADKPVYVYSYGNEVYINASRPEAGNCSVYI
                     YDALGRVVSRGNYTPVAGNHRFTTLATPGAYIVKVISDSGIVTAKIIIP"
     gene            690..926
                     /locus_tag="PKI35_11085"
     CDS             690..926
                     /locus_tag="PKI35_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44970.1"
                     /translation="MKKILLIIIFVFSTTVYNQAFATTPPPPPGTPGMGGGHGTGGNN
                     GPSAPIGGGLEILLLLGAAYAGRKLYKSGQEEEE"
     gene            2512..4125
                     /gene="lnt"
                     /locus_tag="PKI35_11090"
     CDS             2512..4125
                     /gene="lnt"
                     /locus_tag="PKI35_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNX44971.1"
                     /translation="MMAGKKINILLLLVFPLITGMLLGISALPSRLWYVSFVAFIPLL
                     AASERALTCKKPLLVFGLQLLMALIVFYLYVGRWVLQTANLGFLLGLMIVLPFVITVS
                     PFILFRKEGQKVAPVYFISAWLASELFQSHFQLGSPFYNLGHNLGACPRLIQWYEYTG
                     SAGGTLWILASNVALFSVGKDLIYNRKRLFRKSVGALGILILPMAVSLLIYHGYKEKG
                     PSSEVLIVHPSTDCRGIKYRLNIYELMDRYLEIMEPQFTGNTEYVVLPETAITNSGWV
                     ANLNNNLVFDHFLQRTSDFPGLKLVTGAIIYDAVPEAEKTNNLGKLPGIRYSRKYNAW
                     YYTYNAALQIEKDKPVQMRTKEGLVPYQEYAPYPMLLPRIVPVGVDFQFSVRKVNNTV
                     FTAENRRKTAAMICYELVYGNKFCRMARRGAQAFFVLLNEGWYVESPAVKRQFLQLSV
                     IRAIENRRYIAHASNLGISAFVNQRGEVVARNESKQPGFLKHEIRLNKRCTVASSLGD
                     YLGAIALVSMILMLLKEIITKISHRQFQK"
     gene            4138..>6028
                     /locus_tag="PKI35_11095"
     CDS             4138..>6028
                     /locus_tag="PKI35_11095"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX44972.1"
                     /translation="MIRHSTITRSGMVKSKKPDKSILKGSGLTALIFFLLFIPTAKNM
                     YGCWAEDPDADFSANTTTPCVGQSVTFTDNSDPGTYTITSWNWSFGSGASPATATGKG
                     PHNVSYTSSGSKNVALTVHWSGSNSNTETKNGYITANALPTPTISPAGAVCANSAGNV
                     YTTQASMSNYSWTVTGGTITAGGTSNSNTVTVTWGAAGTGHVKVNYSNASGCSAASPV
                     DQSITINALPAPSINPVTMACENSAGNVYTTQPLMSNYSWTVTGGTITAGGTSTSNTA
                     TVTWGAAGTGHIKVNYTNANGCSAASPTDQAITIGPSPAPAFIAQPVANCCMGENETY
                     TTEPGQSNYSWNIPGTSGIDYAIVSGGIGLADNTVTLRWLTPGVKTVTVNYSTSNGCQ
                     GASAASSTVISNYSSLLWKGGDSSDPDNWNLAGNWQVSSSNTEAPPAPPCECTDVIVP
                     RLGSGSTNYPTIVSGSGAVCNNLTIKDGASLIGNEYLTIKGVATVEKTISEAGWYWHF
                     LSSPVESQDIWSQFTPVPNGNSYSNWNWPVSGINWDFYYYNPKVENVYPNVPWVNIRK
                     AEESGSFPYNSGTVDDASPNGTGADAGFGPAKPVFTVGRGYLAAYNSGYSPSTHYFTG
                     VLNSGD"
BASE COUNT         1591 a         1502 c         1475 g         1460 t
ORIGIN      
        1 atagttacag ggatgaagta atgatcgatt tcaacgatca gtattccgga gaagggggtt
       61 cctataaatt cggcagcctg tatgccgaag cgcccgagtt gtggtcggtg aaaaacggcg
      121 tcagttacac catcgaccga tacaaggaga tcacatccgg ccttgccgtg catatcagcg
      181 ccaaatgcgg cgtaaccggc gcttataccc tcacggccac caatatcggc gatttcagcc
      241 tttccaacat cgtttacctc gaagacctga agacggggac gaaggtggac ctgaaggctg
      301 tggggtctta ctcttttacc ggcagtccca acgacaacaa ggaccgtttc agggtgacct
      361 ttgccgagat tgccggcgcc gaagagccgg ccgccgataa accggtatat gtttacagtt
      421 acggcaacga ggtttatatc aacgccagcc gtcctgaggc aggcaattgc tccgtgtata
      481 tttacgacgc cctgggccgt gtggtcagtc gcggaaacta cacgccggtt gccggcaatc
      541 acaggtttac cacgctggca acgccgggcg cttacatcgt gaaggtaata tcggacagcg
      601 gtattgtcac cgcaaaaatt atcatacctt agccgcttac cggccaattt attatggttt
      661 attttcaccc aaacaacagg atccaggcga tgaaaaagat acttctcatt ataatttttg
      721 tgttttcaac aacggtttat aaccaggctt ttgccaccac gcctccgccg cctccgggga
      781 ccccgggaat ggggggcggt catggcaccg gcggaaacaa tgggccttca gccccaattg
      841 gcggcgggct ggagatattg ctattgctgg gcgctgccta tgccggacgg aagctgtaca
      901 agtcagggca ggaggaagag gaatagccgg cagggccttt atgaagccgg catggctgaa
      961 ggttgccgga aaccggcgac agaacaactt caatagggtt aaaagtatca tccatgaacg
     1021 atggctcccg gggatggggg ccattttttt tcgggatttt taaacctggc agcgtcggca
     1081 gacccatcag cgtttgaaaa tccgtaaaca ggtttttcct tgaagcatgg ggaaaagcag
     1141 ggaaaaacgt gagaccgtga atcataaaat aagcattccc gcggttctga cgccgacagg
     1201 tcagaacctc catacccctg caatcacgat cagaataact cttcgatcag ccctgtccaa
     1261 agaatgcatg ctgtagaatg cagtaacgga aagtaaatca gttgcaggga gatgaaattc
     1321 agttaaataa caaccgattg ttcataactt ggccggaatg gcatccgtca caggctgacg
     1381 gctgtattat atttgccccg cttcctgcat atcaggctca tacggtttgt ttaccggccc
     1441 gctctgttct gcaggattgt aaaccttgtt gcacacaagc cgggggaaaa cacaaagaac
     1501 agccaacgta aagccgggaa accctatatt gaaagaaaag aaaattttgt atttttaccg
     1561 gcttaattca gaaagacatt ttgatacgga aaataacagc agaatgaaaa cgccttatcc
     1621 ctgaccggtt gaaatagcgc cggccggata aacccaacac tttgccgctc tttaatttcc
     1681 tttcattttc aaaccgtcag gatcgctcaa cctgaccata ctacgcaaaa ttaaaatcgc
     1741 tcaactaaaa taaatcactc aatcgctcga acaaacttaa attcaaaaaa tcgctcaaaa
     1801 cctgactgaa atgatcagac aggctggctc ttaaactcga aaaccgctcc gatcgctcaa
     1861 aagaatttgt aattgaacct tccgccctct ctggctgtca aagccaggca ggctgtcaaa
     1921 cctgccaatc gtttccacct ggcaggtata cctgaagcat tcctttactt ttactgctct
     1981 cctgtcgcag gcagtttaag ccttgcccaa ggcatgtcag tttgaccttt tccgcgtaaa
     2041 ccaaaaacag gcttctcatc ccgggccctg aaagtggttt gtttcaaaag gccggcatca
     2101 ggagatgcag ataatcagga actgtcatat ccggctatca tcccggaacc gtggattgag
     2161 aatatccgga tgcagacaat gcctcgcgga atacctgtgc agtatcccgg aattcctgaa
     2221 acgaaccggg gctcgtccgg aagaggaatg aagttcatac tcaattgaat gatccgggca
     2281 acctgaaata gcgacgccgg aaaatccgat tgacagaatg accggatgcc ggtgtgaaac
     2341 cacccaaaaa ttgaataagt accgggaaat tttatcccgt caatacgaaa tcacttctat
     2401 gcataccgat attgccgtaa aacacatact gtaccggaat ttcatcatgg agcctgtatc
     2461 tggctcatct gcgggaaaac tattcaacaa ttctgcaatt catccccttg catgatggca
     2521 ggtaaaaaaa tcaatatcct ccttttgctg gtatttccgt tgataaccgg aatgctgctg
     2581 ggcatttcag ccttgccttc caggctctgg tatgtcagct ttgtggcctt tattccgctt
     2641 ctggcggctt ccgaaagggc gctgacctgt aaaaagccgt tattggtttt tggccttcag
     2701 cttctgatgg ccttgatcgt gttttacctt tatgtcggcc gttgggtatt acaaacagcc
     2761 aacctgggtt ttctgctggg attaatgatt gtcctgccgt ttgttattac agtttcaccc
     2821 tttatcctgt tcaggaaaga ggggcaaaag gtggctccgg tatattttat ttcggcatgg
     2881 cttgcctcag agttatttca atctcatttc cagctcggat ccccatttta taacctggga
     2941 cataacctgg gggcgtgtcc ccgcctgatc caatggtacg aatatacagg atctgcaggc
     3001 ggcacactct ggattctggc ctccaacgtt gcactttttt ctgtaggaaa ggatctgatt
     3061 tacaatagaa aacgtctgtt cagaaaaagt gttggagcgc tggggatatt gatccttccg
     3121 atggccgttt cgcttttgat ttatcacggt tacaaagaaa aaggcccttc ttccgaagtg
     3181 cttatcgtgc atccgagcac cgattgccgc gggataaagt accgtctcaa tatttatgag
     3241 ctgatggaca gatacctgga gatcatggag cctcagttca ccggtaatac tgaatatgtt
     3301 gtgcttcccg agacggccat caccaattcc ggttgggtag cgaacctcaa taataacctt
     3361 gtatttgacc atttcctgca aaggaccagc gatttccccg gattaaagct ggtcaccggg
     3421 gcgataatct acgatgctgt tcctgaggca gaaaaaacaa acaacctggg gaagctgccg
     3481 ggtatccggt attcacggaa atataacgca tggtactaca cctataatgc cgcgcttcag
     3541 atagagaagg acaaaccggt gcagatgcgg acaaaagaag gattggtccc ttatcaggaa
     3601 tatgctccct atccgatgct tctgccccgg attgtgccag tcggcgtaga ttttcagttt
     3661 tcggtgcgga aagtgaataa tacggtcttt acggctgaaa acaggagaaa gaccgccgcc
     3721 atgatctgct acgagctggt ttacggcaat aaattctgca ggatggcgcg gcggggcgcg
     3781 caggcattct ttgtactcct caacgaaggg tggtatgttg aatcgcccgc tgttaaacgt
     3841 cagtttttac aactttcagt catcagggcc attgagaacc ggcgttacat cgcccatgct
     3901 tccaatctcg gcatatcggc gtttgtaaat cagcggggtg aagtggtcgc caggaacgaa
     3961 agcaaacagc ccgggttttt aaaacatgaa atcaggctga ataaaaggtg taccgtcgct
     4021 tcctccctgg gtgactattt aggcgcgata gcccttgttt caatgatcct tatgctttta
     4081 aaagagataa ttaccaaaat cagtcacaga caatttcaaa aataatggag gtttaccatg
     4141 attcgtcact cgaccatcac cagatcagga atggtgaaaa gtaaaaaacc tgacaaatca
     4201 atcctgaagg gttcagggct gacagcgctc atattctttt tgttgtttat acctacagcg
     4261 aaaaatatgt acgggtgctg ggctgaagat ccggatgcgg atttctctgc aaacaccacg
     4321 actccctgtg tcggccaatc cgtaacattt accgacaatt cagaccccgg aacctatacg
     4381 attacttcct ggaactggag ttttggttcg ggggcgtctc ccgcaacggc caccggcaag
     4441 gggccgcata atgtttcata cacttcttcg gggtctaaga atgttgcgtt aacggtacac
     4501 tggagcggaa gcaacagcaa tacagaaacc aaaaacggct acatcaccgc gaatgcgctc
     4561 ccgacgccaa ccataagtcc ggctggtgct gtatgtgcaa attcggctgg taacgtttat
     4621 actacacagg cttcgatgtc gaactattcc tggacggtga ccggaggaac aatcactgct
     4681 ggtggcacca gcaattccaa cactgttacc gttacctggg gcgcggcggg aaccggtcat
     4741 gtaaaggtaa attactccaa tgcaagtggt tgcagcgccg caagccctgt tgaccagagt
     4801 atcaccatca atgctttgcc ggcgccttcg atcaatccgg ttaccatggc ttgcgaaaac
     4861 tcggcaggga atgtttacac aacgcagcct ttgatgtcga actattcctg gacggtgacc
     4921 ggcggcacta ttacggcggg aggcaccagc acatcaaata ctgcgacagt tacctggggc
     4981 gcggcaggga ccggtcatat caaggtaaat tacacaaatg caaacggatg cagcgcggcg
     5041 tctcccactg atcaggctat taccatagga ccttcaccgg cgccggcatt tattgcacaa
     5101 cctgttgcca attgttgtat gggggagaat gaaacctaca ctaccgaacc gggtcaatcg
     5161 aattattcat ggaatatacc cgggacttcc ggtattgatt acgccattgt ttcaggagga
     5221 attgggctgg ccgataatac ggttaccctg agatggttaa cgccgggagt caagacggta
     5281 acggttaatt actcaaccag taacgggtgt cagggagcca gcgccgcctc cagtacagtt
     5341 atcagcaatt acagttccct cctttggaaa ggcggcgaca gcagcgatcc ggacaactgg
     5401 aacctggcag gaaactggca ggtatcctcc tcaaataccg aagctcctcc tgcgcctccc
     5461 tgcgaatgta cagatgtcat cgtgcccagg cttggatcgg gaagcacaaa ttacccgacc
     5521 atcgtttccg gttcaggcgc tgtatgcaac aaccttacca tcaaagatgg cgcttcgctg
     5581 atcgggaatg aatacctgac gataaaaggc gttgctacgg ttgaaaaaac catcagtgaa
     5641 gccggctggt actggcactt cctttcatct cccgtggagt cgcaggatat ctggagccag
     5701 tttacacccg ttcccaacgg caacagttat tcaaactgga actggccggt ttccggcatc
     5761 aactgggatt tttattacta taacccgaaa gtcgagaacg tatatcccaa tgtgccctgg
     5821 gtcaatatca ggaaggctga agaatcaggc agttttccct ataattccgg aacggttgat
     5881 gatgccagcc cgaacgggac aggcgccgat gcaggtttcg gaccagcaaa gccggtgttt
     5941 acagtcggac ggggttacct ggcagcctat aattcaggtt attcaccttc aacgcattat
     6001 tttacaggcg tcctgaattc cggggatg
//
LOCUS       DAOPYV010000111         5740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_17482_length_5740_cov_10.119789, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000111 DAOPYV010000000
VERSION     DAOPYV010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17482_length_5740_cov_10.119789"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..134
                     /locus_tag="PKI35_11100"
     CDS             12..134
                     /locus_tag="PKI35_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNX44973.1"
                     /translation="MSHSVQRTYTGFYLDAKSEKARQARLEKIVDRLNRNLKPM"
     gene            575..1453
                     /locus_tag="PKI35_11105"
     CDS             575..1453
                     /locus_tag="PKI35_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44974.1"
                     /translation="MKNLITIFFAILALQISAQDFALIGALWHYDEGTANSELISYET
                     IESIADTMISTVSCRKLIKTSRYGLTPVLSDIFMYSRNDSVFFLADNEFHLLYDFSAD
                     KGDTIILGYYTNHDGTALKMIIDSTSEISINGQIRKLQYVTSGDGISIEFGGIVISGI
                     GNIEYMFPHYDMWNEGPLRCYEDSICGLFKNSRHTNNGWNFQDCDQIITGINEAGLAN
                     GVSLYPNPTTSLLNISNLDQKTEYVIIECYGRSLKGGFLLPSETINIEDLPDGIYSIL
                     LINNKIKTITRRIIKL"
     gene            1559..2401
                     /locus_tag="PKI35_11110"
     CDS             1559..2401
                     /locus_tag="PKI35_11110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX44975.1"
                     /translation="MKTLKSIILMILIPFSVSAQDFMTVRDIFNFEIGDEFHIEGRGL
                     NQPPNADRITITCKYYSPDSNTVYYVRDHDSYYVTIENYETHYHFWTKTDTVSYSNLE
                     LPVNQSEYWITYDTAMVIYDTINAIFGDYCQKLINGYYYAWNPFEPVYYSSLYGEGLG
                     LVRSYYYEPAGSTMYDNVLFYYKKNGVCCGIPDTTTLSVPENHFLKGFNIYPIPAKSY
                     LIIENKRNLEIQSITLFDLNGNIIRQFDLNSSHLDVPDLASGFYILKVSSKSGVLMKK
                     IVIE"
     gene            complement(2572..3573)
                     /gene="argF"
                     /locus_tag="PKI35_11115"
     CDS             complement(2572..3573)
                     /gene="argF"
                     /locus_tag="PKI35_11115"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNX44976.1"
                     /translation="MAFNLKNRNFLKELDFTPEEIKFLLDLAFELKKAKYAGTEQPRL
                     KGKNIVLLFEKDSTRTRCAFEVAALDQGAHVTYLGPSGSQMGKKESMKDTARVLGRMF
                     DGIEYRGYAQTIVEALAEYSGVPVWNGLTNEFHPTQMLADFMTMMEHTDKPLSKVSFA
                     FLGDAKNNVGNSLMVGAAKMGMDFRAVAPKSCQPAAELVEECREIAEVTGARITITDN
                     VEKGVKGCDFLYTDVWVSMGEPFEYWEERIKLLKPYQVNADVVRKTENPNVKFLHCLP
                     AFHNRETVVGEDIFQKFGLNGMEVTEDVFESPMSIVFDEAENRLHTIKAVMVATLGA"
     gene            complement(3658..4602)
                     /gene="arcC"
                     /locus_tag="PKI35_11120"
     CDS             complement(3658..4602)
                     /gene="arcC"
                     /locus_tag="PKI35_11120"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNX44977.1"
                     /translation="MKKLAVVAFGGNALLRGDQAGTIEEQEKNARETCERLLTLINRD
                     YNVVLTHGNGPQVGNILLANSAGKQVYNLPEMPMDVSVAYSQGFIGYVLEQQLRNVLR
                     EHELERDIITIITQVMVDKDDPAFENPTKPVGPFYPKEVADKMSEATGSVFKEDPRGR
                     GWRKVVASPKPLVISNQRSIEKLARDGQIVIAVGGGGIPTFYVAENKLQGIDAVIDKD
                     LASALLAGNINADKLFILTDVPKVCINFNTPQEKSIDRMNVKQAKKYLEEGHFAEGSM
                     APKIRAAIHFVKHTSRDAIITSANVLGVDNGGTRISIV"
     gene            4781..5689
                     /locus_tag="PKI35_11125"
     CDS             4781..5689
                     /locus_tag="PKI35_11125"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX44978.1"
                     /translation="MHPQRKAYLLALLSIGFWCTMGTAFKLTLRHINPPLLLFFATSV
                     AFIFLFVVSAFSGRLKSVRALSGRDLLSSALMGLFNPFLYYMVLFKAYSMISAQEAVT
                     LNYLWPVMLVIFSIIFLRQRVTVAGFCAILISFSGTVIIAAHGDLSNFSFASLPGDCL
                     AAGSSLFWASFWILNMKDRRDDIAKMMMNFLFGLIYIAVWNLATGNLALPSVKALAGS
                     VYIGIFEMGLTFVVWLTALKLSSNTARISNLVYISPFLSLVLVSVFVGEKILASTVLG
                     LVVIVSGILLQAYANRKIRREQPKEE"
BASE COUNT         1499 a         1296 c         1312 g         1633 t
ORIGIN      
        1 atttgctggc tatgtcgcat tcggtgcagc gtacctatac cggattttat cttgacgcca
       61 aatcggaaaa agcccgccag gcgcgactag aaaagattgt cgaccggctt aacaggaatt
      121 tgaagccaat gtgatgtaag tcatttatag gatactattt attaaggaaa agactgataa
      181 aaggcacgaa cacataccca tgggataatg catgcgggtt tcgtttgttt tcaaaggttt
      241 ctaactcgta aaaagacgaa aaatggcgac cgtctccggc cgccattgct atcaaatatg
      301 tgcagggcta ttaagctccc agcgtcgcca ccattacggc tttgatggtg caggcggtta
      361 ccggcttcgc cgaaaatgag atgaattcac aaactattta aataatttca ggaacattaa
      421 aaaataaatt tccatatatt cgtgtgttga ttttatcttt tgaagggcaa tcttatcaaa
      481 tagaagatat tgaaggaatt ataaaagaat ttaagaaaag acagaaaaca taaaacactt
      541 cataacatgc attattaacc tgactttatg cattatgaaa aatttaatca caattttctt
      601 cgccattctg gcacttcaga tttccgcaca ggattttgca ctcatcgggg ctctgtggca
      661 ttatgatgag ggaactgcaa attcagaact catttcatat gaaaccattg aatctattgc
      721 cgataccatg atcagtactg tatcatgccg gaaactgata aagacttcgc gatacggttt
      781 aactccggta ttatcagata ttttcatgta ctcaagaaat gatagcgttt tctttttagc
      841 tgataatgaa ttccatctgt tatatgactt ttcagctgac aagggagata ccatcatcct
      901 cgggtattat acaaatcatg atggtactgc actaaaaatg atcatcgatt caacaagtga
      961 gatttcaatc aatggacaga tcagaaaatt gcaatatgtg acttcggggg atggaatttc
     1021 cattgagttc ggcggaatag tcatttcagg aatagggaat atagaatata tgttccctca
     1081 ttatgatatg tggaatgaag gaccattgcg ttgttacgaa gacagtattt gcggattatt
     1141 taagaattcc cggcatacca acaatggttg gaattttcag gattgcgatc agataataac
     1201 cggaattaat gaagccggtt tagcaaacgg agtatcactt tatccgaatc cgaccacttc
     1261 acttttaaat atttctaacc tcgaccaaaa gacggaatac gtgattattg aatgttatgg
     1321 ccgatcatta aaaggtggat tcctgctgcc ttcagaaacc attaatattg aggacctgcc
     1381 cgatggaata tattctatct tgctgataaa taacaaaata aagacaatca caaggaggat
     1441 aattaaacta tagcgcatgt taatacgggc taagcaagac gagtttcccc actccgacag
     1501 gcccaggaac gtatataaac aacataagag taaataccaa ccgctaaaac ttgaagccgt
     1561 gaaaacactg aagtcaatta ttctgatgat tttgatacca ttttcagttt ctgcacaaga
     1621 ttttatgacc gttcgagaca tattcaattt tgagattggt gatgaatttc acatagaagg
     1681 aagaggatta aatcagccac cgaatgcgga tagaattact attacctgca aatattattc
     1741 tcctgacagc aatactgtat attatgtccg ggatcatgac tcttattatg ttacaatcga
     1801 gaattatgaa acacattatc atttttggac aaaaacagat actgtttcct attcaaacct
     1861 ggagttgccg gttaatcaaa gtgaatattg gatcacatat gatacagcta tggtcattta
     1921 tgatacaata aatgccattt tcggcgatta ttgccaaaaa ctgataaatg gatattatta
     1981 tgcctggaat ccgtttgaac cggtatatta cagctcactc tatggagaag ggttaggatt
     2041 ggtcagaagt tattactatg agcctgccgg gtcaacgatg tatgacaatg tactttttta
     2101 ttataaaaag aatggagtat gttgtggcat tcctgatacg acaactttat ccgtacctga
     2161 gaatcacttt ctaaagggtt tcaatattta tcccattccg gcaaaaagtt acctgatcat
     2221 tgaaaataaa aggaaccttg aaattcaatc catcaccctg tttgatttaa atgggaatat
     2281 catcagacaa tttgacttaa actcatcgca tcttgacgtc ccggatttgg cttcagggtt
     2341 ctacattttg aaagtatcaa gtaaatcggg ggtgttgatg aaaaagatcg taatcgaata
     2401 aaagctataa actgcgtttt ccaataaaca ggaatatgag ccgtctgcaa tccctgtcct
     2461 ggaaaggaag agcggcgatt attcccggta ttactagccc gcatctattc ccgatagaaa
     2521 aaaaatggcg accgtctccg gccgccattt ctataaatat atttagggct attaagctcc
     2581 cagcgttgcc accatcacgg ctttgatggt atgcaggcgg ttttcggctt cgtcgaaaac
     2641 aatcgacatc ggcgattcga aaacatcctc ggtaacttcc atgccgttga ggccgaattt
     2701 ctggaagata tcctccccta ccaccgtttc gcgattatgg aacgccggca ggcaatgcag
     2761 gaatttcacg ttcgggtttt cggttttacg gacgacatcg gcatttacct ggtaaggttt
     2821 cagcagttta atgcgttctt cccagtattc gaaaggttcg cccatcgata cccatacgtc
     2881 ggtataaagg aagtcgcagc ctttcacgcc tttttccacg ttatcggtaa tggtgatgcg
     2941 tgcgccggtg acttctgcaa tttcgcggca ttcttccacc agttcggcgg ccggttggca
     3001 actctttggg gcaacggcgc ggaagtccat gcccatcttg gcagcgccta ccatcagcga
     3061 attgcccacg ttgttcttgg catcgcccag gaaggcaaag cttaccttgc tcagcggctt
     3121 gtcggtgtgt tccatcatgg tcatgaagtc ggccagcatc tgcgtgggat ggaattcatt
     3181 ggtaagcccg ttccataccg gtacgccgga atattcggcc aacgcttcca cgatagtctg
     3241 ggcatagccg cggtactcga tgccgtcgaa catgcgtccc agcacgcggg cggtatcttt
     3301 catggattcc ttcttgccca tctgcgatcc ggaagggccc aggtaggtaa catgggcgcc
     3361 ctggtcgagg gcggctactt cgaaggcgca acgggtgcgg gtggagtcct tttcgaaaag
     3421 aaggacgatg tttttacctt tcagccgagg ctgttccgtt ccggcatatt ttgctttttt
     3481 aagttcgaaa gcaaggtcga gtaaaaattt tatctcctca ggggtaaaat ccagttcctt
     3541 gaggaaattc ctgtttttaa ggttgaaagc cataggaata tattgattta aggttagtcg
     3601 ttagtcggtt gtttcaatgc ttcgggatcg gatgtgtccg gaatccggaa cgccccgtta
     3661 tacgatcgaa atcctggtac cgccgttgtc aacgccaagc acattggccg aggtgatgat
     3721 ggcatcgcgg ctggtatgtt tcacaaaatg gatggctgcc ctgatcttcg gcgccatgct
     3781 gccttcggca aaatggcctt cctcgaggta ttttttagcc tgtttcacat tcatgcggtc
     3841 gatggatttt tcctgcggcg tgttgaagtt gatgcagacc ttgggcacat cggtaaggat
     3901 gaagagtttg tcggcattga tatttccggc cagcagggcg gaagccagat ctttgtcgat
     3961 cacggcgtcg atgccctgaa gcttgttttc ggcgacatag aaagtgggga tgccgccgcc
     4021 gccgacggca atgacgatct gcccgtcgcg ggcaagtttt tcgatggaac gctggttgct
     4081 gatcaccagg ggtttgggcg aggccaccac cttgcgccaa ccgcgtccgc gcggatcttc
     4141 cttaaagacc gatcccgtgg cttcactcat tttgtcagca acttctttcg gataaaaagg
     4201 tccgaccggt ttggtgggat tttcaaaagc gggatcatct ttgtcgacca tcacctgggt
     4261 gatgatggta atgatatcgc gttccagttc atgctcccgc aatacgttac gcaattgttg
     4321 ttcaagcaca taacctataa aaccctggga ataggccacc gacacgtcca tcggcatttc
     4381 gggaagatta tatacctgtt ttccggccga gtttgccagc aggatattac ccacctgcgg
     4441 gccgttgcca tgcgtaagca ccacgttata atcccggttg atcagcgtaa gcagccgctc
     4501 acaggtttcc cgcgcatttt tttcctgttc ttcgatggtt cctgcctggt cgcccctgag
     4561 cagggcattt cctccaaatg ctacaacagc aagttttttc ataagctttc cgttattaaa
     4621 atgcaaaagt agcattattt ccgtagtcag caaatcgccg ggaacgccct cctaccttac
     4681 actcaatctg tgcgttagcg attgccggac cggaatcata accgatgtgc ctgacgagtg
     4741 acatgaattt atttgcattt ttgcatttaa accatacggg atgcatccac aacgaaaagc
     4801 atatctgctg gcgctgcttt cgatcggttt ctggtgtacc atgggaaccg ccttcaaact
     4861 gacgctccgg catatcaacc ctccgctgct gctttttttc gcaacatccg ttgcctttat
     4921 tttcctgttt gtggtatctg cattctcggg caggctgaaa tccgtaaggg ccctcagcgg
     4981 cagggacctg ctttcctcag ccctgatggg gttgttcaac ccgtttttat attacatggt
     5041 gttgtttaag gcttacagca tgatctcagc ccaggaggcg gtcacgctga attacctgtg
     5101 gccggtgatg ctggtaatct tttccatcat ttttctcagg cagcgcgtta cagtcgccgg
     5161 tttttgcgcc attctgatca gtttctccgg aacagtgatc atcgctgcgc atggcgacct
     5221 gagcaatttc agttttgcca gcctgccggg agactgcctt gccgccggaa gttccctttt
     5281 ctgggcttcg ttctggatcc tgaacatgaa agacaggcgc gacgatatcg ctaaaatgat
     5341 gatgaatttc cttttcggcc ttatctacat cgccgtctgg aacctggcaa cgggaaattt
     5401 ggccttaccg tccgtgaaag ccctggccgg ctcggtgtac atcggcatct tcgaaatggg
     5461 gctgaccttt gtggtatggc ttaccgcgct gaaattgtca tcaaacacag cccgcatttc
     5521 caacctcgtt tatatctctc cttttctttc actcgtgctt gtctcggtct ttgtgggaga
     5581 gaaaatactg gcatccaccg tgctggggct tgtcgttatc gtaagcggga tactgcttca
     5641 ggcttatgca aacaggaaaa tacgccggga gcagccgaag gaagaataat atcatcgctg
     5701 aatactgacg gcaggttcgc ctctgtagat cccatttttt
//
LOCUS       DAOPYV010000112         5521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_18502_length_5521_cov_13.728869, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000112 DAOPYV010000000
VERSION     DAOPYV010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18502_length_5521_cov_13.728869"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PKI35_11130"
     CDS             <1..381
                     /locus_tag="PKI35_11130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNX44979.1"
                     /translation="HKNISEFHVVDTMSERKAMMNDLADAFIAMPGGIGTLDELFEVM
                     SWNQLELIDKPVALFNINGYYDNLITFLDHSVEQRFVKAEHRQNLICESDANRLLEKI
                     ENYKAVKVEGGKWIKELKENTFQA"
     gene            445..1014
                     /locus_tag="PKI35_11135"
     CDS             445..1014
                     /locus_tag="PKI35_11135"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX44980.1"
                     /translation="MLRKMLIIGITGTLGAGKGTIVDYLVKEKGFIHFSVRGFISEEI
                     IRRGMPVNRDSMVVVANELRALHSPSWITDQLYEQALLAGKNSIIESIRTPGEVESLR
                     SKGNFLLFAVDADPRLRYERIVKRNSETDHISFATFLEDEKREMASSDPNKQNIGKCI
                     KLADYTFMNDHSIESLQAEVESVLQKLFI"
     gene            1117..2088
                     /gene="wtpA"
                     /locus_tag="PKI35_11140"
     CDS             1117..2088
                     /gene="wtpA"
                     /locus_tag="PKI35_11140"
                     /inference="COORDINATES: protein motif:HMM:NF003196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungstate ABC transporter substrate-binding
                     protein WtpA"
                     /protein_id="HNX44981.1"
                     /translation="MKALNFLFPVLFLAAVASYPAAVFGQNAGRVSGDLIIFHAGSLS
                     VPLKQVADEFKKANPGVNIMMEAAGSVASARKITDLGKPCDILASADYAVIDKMLIPK
                     YADWNIKFAGNEMCVVYNENSKYSDKISAKNWMNILRQPDVIFGRSDPNADPCGYRTV
                     MMLQLAVKYYKKPGLEKQFLGKDVAYMRPKETDLLALLETGSVDYIFLYRSVAVQHNL
                     KYIILPDQINLKNPAFAGEYAQATVLINGNEPGVKVTMTGEPMIYSFTIPRNAPNKAA
                     ALAFAEFLLSKEKGQKIMLRNGQPSVVPMKVNHFEKVPARLRPFVKR"
     gene            2547..3338
                     /locus_tag="PKI35_11145"
     CDS             2547..3338
                     /locus_tag="PKI35_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX44982.1"
                     /translation="MRSPFQLTITLASALILLFIIAPLVGMFVATSPVELFDTAKDAE
                     VQQSIKLTIWVSLAATVIFSLAAIPFAFLLARKKFPLKSIVTGIIDLPIVIPHSAAGI
                     AILGFVSRNSVLGKAGSAIGLDFVGHPAGIAVAMAFVSLPFLINSARDGFAGVPERLE
                     KAALTLGASPSRVFFSVSLPLAWRNIASGLILMFARGMSEFGAVIMVAYHPMISPVLI
                     YERFGAFGLKYARPVSVLFILISLAVFVALRLLSRRQEPKTAGHA"
     gene            3331..4383
                     /locus_tag="PKI35_11150"
     CDS             3331..4383
                     /locus_tag="PKI35_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX44983.1"
                     /translation="MLSLQHISVSFPGFQLRDISFDVAGGEYFILLGESGAGKSLLLE
                     TIAGLNKQQSGTITLSGKDITHEKIQQRCIGLVFQDHAVFPHLSVKENIVYPLKHKHF
                     SAEEKASRIQPVIETLQIGHLIHRRPPTLSGGELQRVALARALVQQPQVLLLDEPLAS
                     IDSKLKSDLRALLRQINRMGQTIIHVTHDFDEAVSLADRIAIIDKGSIVQTGTPHEVF
                     SNPVNGFVAHFAGIRNFFKVRIEKQPGHITVFPHEKVPVVLNQETDAEEGFLLIRNED
                     ILISEENFESSALNNFKGIVEDVYPLRYGMEVVVNIGIPLHVQVSRESAEKLRLVAGK
                     PVWTHFKASSARLICG"
     gene            complement(4572..>5521)
                     /locus_tag="PKI35_11155"
     CDS             complement(4572..>5521)
                     /locus_tag="PKI35_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44984.1"
                     /translation="ILLILNFNGMLFVPTFAVELISYTLPMRTCGFTIVRNAVKYDYP
                     VVESIKSVLPVCDEFLVLAGNSDDNTLDLIRQIGSPKIRIAESTWDDSLKEGGRVLAA
                     ETNKAFDLISPEFDWALYLQADEVIHEKYHDNIREAMKKWLDCPEVEGLLFNYTHFYG
                     TYHYTGDSRRWYRHEIRIIRNDKSIRSYRDAQGFRKKGEKLRVRPVDASVYHYGWVKN
                     PKTMQTKVLDFHKLWHDEEWIKQNVNQEELFDFTDVDSLAVFTGTHPAVMTDRIKNAD
                     WNIELDISKKKLGFKNRILLLFEKLTGYRLFEYKNYRII"
BASE COUNT         1409 a         1343 c         1312 g         1457 t
ORIGIN      
        1 cataaaaaca taagcgaatt tcatgtagtg gataccatgt cggaacgcaa agccatgatg
       61 aatgacctgg cggacgcttt tattgccatg ccgggcggca tcggcacact cgacgagttg
      121 tttgaagtga tgagctggaa tcagttggag ctgatcgaca aaccggtagc gctgtttaac
      181 atcaatggtt attatgataa cctgatcacg tttctcgatc attcagttga acagcggttt
      241 gtaaaagctg aacacaggca aaaccttatc tgcgaaagcg acgccaaccg tctgcttgaa
      301 aaaattgaaa attataaggc ggtgaaagtt gaaggcggta aatggattaa ggaattaaaa
      361 gaaaatacat ttcaggcata agaagccgtt gtatctttac agcgaatctt atcataataa
      421 aaatactatc tatcgggtag tcagatgctc cgtaaaatgc tcatcatagg aatcaccgga
      481 acgcttggcg ccggcaaagg aaccattgtt gattacctcg taaaggaaaa gggatttatc
      541 catttttcgg tacgcggatt tatctcggaa gagatcatac gccgtggaat gcccgtgaac
      601 agggatagta tggtggtggt tgccaatgaa ttgcgcgcct tgcattcgcc ttcctggatt
      661 acagaccagt tgtatgaaca ggcgttgctg gccggcaaaa acagcatcat cgaaagcata
      721 cgtactcccg gcgaggtgga atcgctccgt tcgaaaggca acttccttct cttcgccgtg
      781 gatgccgatc ctaggcttcg ttacgaacgc atcgtgaaac gcaacagcga aaccgaccat
      841 atttcatttg ccacgttcct ggaggatgag aaacgcgaga tggcctcgtc tgatccaaac
      901 aagcagaata tcgggaaatg cataaaactg gcagattata cttttatgaa tgaccattct
      961 attgaaagcc ttcaggctga agttgagagt gttcttcaaa agcttttcat ctgacaggat
     1021 tcccggatta aaaagcatca ctgaccagga accgccggtc aatcccggtg tttactttca
     1081 ttgcttatct ttgcaggtat taaaagcctc aattaaatga aagccttgaa tttcctgttt
     1141 ccggtattat tcctggcagc cgttgcatca tatcccgcag cggtgtttgg tcagaatgca
     1201 ggaagggtat caggagacct gatcattttt catgccggca gcctctcggt gccgttgaaa
     1261 caggttgccg atgaattcaa aaaagcaaat cccggcgtta atatcatgat ggaagccgcc
     1321 ggctcggtgg cttcggcccg caagatcacc gacctgggaa agccctgcga tattctggct
     1381 tcggccgatt acgccgtgat cgacaaaatg ctgatcccga aatacgccga ctggaacatc
     1441 aaatttgccg gaaacgaaat gtgtgtggtt tacaacgaaa actcaaagta ttcggacaaa
     1501 atcagtgcaa agaactggat gaacatcctc cggcagcccg atgtgatctt cggccgttcc
     1561 gatcccaatg ccgacccctg cggatatcgg acggttatga tgctgcaact ggccgtgaaa
     1621 tattacaaaa agccggggct ggaaaaacag ttcctgggca aggatgtcgc ttatatgcgt
     1681 ccgaaagaaa ccgatctgct tgcactgctc gagaccggtt cggtggatta catcttcctg
     1741 tatcgcagcg ttgccgtgca acacaacctg aaatacatca tccttcccga ccagatcaac
     1801 ctgaagaatc ctgcttttgc cggggaatat gctcaagcaa cagtgttgat caacgggaac
     1861 gaaccgggtg taaaagtcac gatgaccggc gaacccatga tttacagctt caccataccc
     1921 cggaatgcgc ccaataaagc cgctgctctg gctttcgccg aattcctgct tagcaaggag
     1981 aaggggcaga aaatcatgct gaggaacggg caaccttcgg tagtgcctat gaaggtgaac
     2041 catttcgaaa aggtgcccgc caggctcagg ccattcgtaa aaagataatt cccggatatc
     2101 gttgctgttg gctcattgtc agccggttga cgccagctca accttacagg cttttctctt
     2161 tggtccggac ttttgccata ctttcgataa actttatact tttgttcttt gtccggattc
     2221 cggtacgaaa tgggataaaa caaccggaaa acccaataag ttacctgaca accggaaaat
     2281 caacagcaaa tcagcaggga gtcttctcaa tacatcgtta aacattcacc aggccatgca
     2341 cgtatcttca tatcctcttt tcctgcttcc ttaacacctg ccgcctgcag gcttacacca
     2401 ggctggtaaa gttgtcggtt aatagtgcct ttagtgcctg caatacctga agttttgagt
     2461 tctccgttct ccatgctccg tgctccgtgc tccgtgctcc aagttctttg ccctttgact
     2521 ttttaccttt atcctttctc ttttcgatgc gttctccatt tcaactcacg atcaccctgg
     2581 cctcggcact tatcctgctg ttcatcattg cgccgctggt ggggatgttc gtggcaacca
     2641 gccccgtcga attgtttgac acggcaaagg atgccgaggt tcagcaaagc ataaaactca
     2701 ccatctgggt ttcgctggcg gcaactgtca tcttttcact ggccgccata ccatttgcct
     2761 ttttgctggc cagaaaaaaa ttcccgctaa agagcattgt cacaggcatc atcgacctgc
     2821 cgattgtgat cccgcactcg gcagcaggta ttgccatcct gggatttgtt tcgcgcaact
     2881 ccgtcctggg gaaagcgggt tcggccatag ggctcgattt tgtaggacat ccggccggaa
     2941 ttgccgtcgc catggctttt gtcagccttc cttttctgat caattctgcc cgtgacggtt
     3001 ttgcaggagt tcccgaaaga ctcgaaaagg cggcgctgac tttgggagca tcgccttcgc
     3061 gggttttctt ctctgtatcg ctgccgctgg catggcgcaa tatagcttcg gggcttatcc
     3121 tgatgttcgc caggggaatg agcgaatttg gcgccgtgat catggtcgcc taccacccca
     3181 tgatctctcc tgttctcatt tacgagcgtt ttggcgcctt tggactgaaa tatgcaaggc
     3241 ctgtttcggt gctttttata ctcatttcat tggctgtatt tgtagcgctc agactgctgt
     3301 cgcgcaggca ggaaccaaaa accgccggcc atgcttagtc ttcagcatat ctccgtttcg
     3361 ttccccgggt ttcagctccg cgatataagc ttcgacgtag ccggcggcga atatttcatc
     3421 ctactgggtg aatccggtgc aggaaaatcg ttgctgctcg aaaccattgc cgggctaaac
     3481 aaacaacaaa gcggcaccat caccctgagc gggaaggata ttacccatga aaaaatacag
     3541 cagcgttgta tagggcttgt atttcaggat cacgctgtgt ttccgcatct atccgtaaag
     3601 gaaaatatcg tttacccgct gaaacacaaa catttttcag ccgaagaaaa agcttcacgc
     3661 atccaaccgg ttattgaaac tttacaaatc ggccacctga tacaccggcg tccgccgacg
     3721 ctctcgggag gcgagttgca acgagtggcg ctagcccgtg cgctggtaca gcaaccgcag
     3781 gttcttcttc tcgacgagcc gctggcatcc attgatagta agctgaaatc ggatttgcgt
     3841 gccctgctga gacaaatcaa ccggatggga caaaccatca tccacgttac gcacgatttt
     3901 gatgaagccg tttcgctggc cgaccgtata gccattatcg ataagggaag catcgtacag
     3961 accggcactc cccacgaagt gttcagcaac cccgtgaacg gatttgtcgc ccactttgcc
     4021 ggaatacgca atttctttaa agtcaggatt gaaaaacagc ccggtcatat taccgtcttt
     4081 cctcatgaaa aagttcccgt tgtcctgaac caggaaaccg atgcggaaga gggattttta
     4141 cttatcagga atgaagatat tttgatttcg gaagaaaatt ttgaaagcag cgcgctcaat
     4201 aatttcaaag gaatagtcga ggatgtttat cccctgcgtt atggcatgga agtggttgtg
     4261 aacatcggca tcccgcttca tgttcaggtt agccgcgaat cggcggaaaa gctccggctg
     4321 gtcgccggca aaccggtatg gacccatttt aaagcctcat cagccaggct gatctgtggg
     4381 tagcattttc cataatttca gcgaattgtg taagaaattc tacatggaag gcccgtaagg
     4441 ttatccggtg gactgaaggg cttcgatgcg tggctatggc gattaaactt cccctcgcta
     4501 aatacggatt attccgactt gatttttgcc gattaataag ccgggttggg ataaaatgca
     4561 gggttgacga tttaaataat cctgtaattc ttgtattcaa acaaccggta accagtcagt
     4621 ttttcgaaca gaagaagtat tctgttttta aatccgagtt tctttttgga aatatccagt
     4681 tcgatgttcc agtcggcatt tttaatccgg tccgtcatca cagcagggtg agttccggtg
     4741 aagactgcca gtgaatcgac atcggtaaaa tcaaataatt cttcctgatt tacattttgt
     4801 ttgatccatt cctcatcatg ccaaagtttg tggaaatcga gcactttggt ctgcatggtt
     4861 ttcggatttt taacccagcc atagtgatat actgaagcgt cgaccggcct gacgcggagt
     4921 ttttcgcctt ttttcctgaa tccctgggca tcgcggtacg accggatgga tttatcgttg
     4981 cggattattc tgatttcatg gcggtaccac cggcgcgaat caccggtata atggtaggtt
     5041 ccgtaaaaat gggtgtaatt aaaaagcagc ccttccactt cagggcagtc cagccatttt
     5101 ttcatggctt ccctgatgtt gtcgtggtat ttttcatgga tcacttcatc agcctgcagg
     5161 taaagcgccc agtcgaactc aggagagatg aggtcgaaag ctttattggt ttctgctgcc
     5221 agcaccctgc cgccttcctt gagcgagtca tcccaggtac tttctgctat gcggatttta
     5281 ggagaaccga tctgcctgat caggtccagg gtattgtcat ctgaatttcc ggccaaaaca
     5341 aggaattcat cacaaaccgg taacaccgat ttgatggatt caaccaccgg ataatcatat
     5401 ttgacggcat ttctgacaat ggtaaatccg caggttcgca taggtaaagt ataactgatc
     5461 aattcaactg caaaagtggg gacaaaaagc ataccgttga aattcaggat taataaaata
     5521 g
//
LOCUS       DAOPYV010000113         5387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_19151_length_5387_cov_12.672731, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000113 DAOPYV010000000
VERSION     DAOPYV010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19151_length_5387_cov_12.672731"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /gene="pdxA"
                     /locus_tag="PKI35_11160"
     CDS             <1..786
                     /gene="pdxA"
                     /locus_tag="PKI35_11160"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNX44985.1"
                     /translation="SSVIAGEMSIQALDAAIEDLKKGLIDVLVTAPVNKSNVAQFMKE
                     HFSGHTEYLAAKLGAADPLMLMVSDKLRIGVATSHIALSEVSPKITQELIISKLQKME
                     MSLKKDFAISKPRIAVLGLNPHAGDNGLLGDEEQTVIKPAIEKVYSKGMFAFGPFPAD
                     GFFASDGYKSFDGVLAMYHDQGLIPFKTLSAGEGVNFTAGLPYVRTSPAHGTAYDIAG
                     KDIASPDSMRAAMYLACDIFNNRRMWEELNRNPLPIAQLEKES"
     gene            872..3361
                     /locus_tag="PKI35_11165"
     CDS             872..3361
                     /locus_tag="PKI35_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HNX44986.1"
                     /translation="MVRNQYLFSDIAQILKGEILNAPDDHSEIHDLLIDSRKLIAPEG
                     CLFFALISKRNDGHKYLEDLYDQGVRNFVVQRAPENLPSFPEANIIRVKDTLRALQLL
                     ASEHRKQFDIPVIGITGSNGKTIVKEWLYQLMSSDKNIVRSPRSYNSQIGVPLSVWLM
                     NPSNEIAIFEAGISEPDEMEHLQAVIQPTIGIFTNLGHAHDENFINRLQKSGEKLNLF
                     TQVDTLIYCTDQKELQEGILKTGLLRKINVFTWSRHEDADLKIIRCQHNHHSTEISGI
                     YRENTISISIPFTDEASADNAIHCWSALLYLGYDNEVIAGRMKSLQPVAMRLEMKEGI
                     NNCTLINDSYSNDIDSLSIALDFLNQQKQHKKKTAILSDILQSGRSDRYLYNEIGKLL
                     ARKDVEQVIGIGEAISKQSGSFSMKKAFFKDTDDFLNSYSFSSFSNECILVKGARVFE
                     FERIIEQLQQKTHETILEINLNAVVHNLNYYRSKLKPGVKLMAMVKAFSYGSGSYEIA
                     NTLQFHQADYLTVAYADEGVELRKAGITLPIMVMNPDEQGFDSMFKYGLEPEVYSMRI
                     LNMLNNAIQQHNPAPGPVGIHVKIDTGMHRLGFDPGEVDDLIRNLKGNSHIEVRSVFS
                     HLAGADDPALDDFTHHQIETLKKTSEKIQKAFDYPILRHIDNTAGITRFPEAQFDMVR
                     LGIGLYGIAPLAPEQENLQNVSTLQTVISQIKHIPADDTVGYNRKWKAKIDTTIGIIP
                     IGYADGLNRRLGNGAGKLLVNGNIVPIVGNICMDMCMVDITGIDAAEGDEVIVFGAGM
                     PITELSGAIGTIPYELLTAISRRVKRIYFEE"
     gene            3375..5237
                     /locus_tag="PKI35_11170"
     CDS             3375..5237
                     /locus_tag="PKI35_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44987.1"
                     /translation="MELSLATGRKGKKTSVSFITCVILILAIVIFRSLYPPVNILTWD
                     VFGYYLYLPAQFIFHDIGFRNLEWVRQIAEQYQTTSTLYQLVTSPDGGFVIKYSAGMA
                     VLNAPAFFIAHLLAQPLGFPADGFSLPYQYAWAINGLIVTLAGIFILRKILLEFFDET
                     VATIVLVIVTLATNYFQLTAFDGYLTHNYTFTLCALITWLTIKWHKRPTYPAACWLGI
                     SLGLIILVRPSEMVCLLIPVLWGVYSRASVKEKLLLVREHPGQVLALILAVFIAGLPQ
                     LIYWKTVTGHFIFYSYVNAGEGFDFANPHTWQVLFSYRKGWLVYTPVMFMAIIGFVSL
                     YRHNRKLLIAVMVYLIVNLYVVSSWTTWWYAGGSYSQRALLTSCVLMALPLGYFIRDM
                     FLRKWSMAVVILMLSLTLLLNLFQTWQWVHGIIDKTRMTRSYYWAVFGKTTVPENASG
                     LLLIDRSEEDYLKNPEKYRSRILKFYDFESSGPEKGNSCGENSFSGSCAVKLDSTIRF
                     SPAVEATFRQLTAADHAWIRISAEVFPLTDPAENPASLVATFEHNGEAYKYQAEGIEL
                     EKPALKTGQWNLVRFDYLTPEIRSENDKLKVYFWLRGNKPILIDDLKVEVWEPK"
BASE COUNT         1435 a         1361 c         1288 g         1303 t
ORIGIN      
        1 tcgagcgtga tagccggtga aatgtcgatc caggccctgg atgctgccat tgaggacctg
       61 aaaaagggcc ttatagatgt acttgtcacc gcccctgtca ataagagcaa tgtggcgcaa
      121 ttcatgaagg aacatttcag cggccatacc gaatacctcg ctgcgaaact gggcgcagca
      181 gatccgctga tgctcatggt cagcgataaa ttgcgtattg gcgtcgccac cagccacata
      241 gccttgtcgg aagtctcgcc gaaaataacg caggaactca tcatcagcaa gctgcagaag
      301 atggaaatgt cgctgaaaaa ggacttcgcg atctccaaac caagaattgc cgtgcttggg
      361 ctcaatcccc atgcaggcga caacggtttg ctcggtgatg aagaacaaac cgtaatcaag
      421 cctgccatcg aaaaagttta cagtaaagga atgtttgcct tcgggccgtt ccccgccgac
      481 ggcttctttg cttccgatgg ctataagtcg tttgacggcg tgctggcgat gtatcacgat
      541 cagggattga tacctttcaa aaccctgtca gccggcgaag gggtaaattt cacggccggg
      601 ttaccctatg tccgcacctc gccggctcat ggaactgctt acgacattgc cggtaaagat
      661 attgcttcac ccgattcgat gcgggcggca atgtatcttg cctgcgacat cttcaacaac
      721 cgacggatgt gggaagaact aaaccgcaac cccctgccga ttgcccaact tgagaaggaa
      781 agctaagcct gccttgatac tgtttttcca acatctttgt tggccggatt gaaccaccct
      841 atgccgggta attccagcaa acctttttaa aatggtcaga aatcaatatt tattcagcga
      901 tatagcccag attctaaagg gagaaattct gaatgcaccc gatgatcatt cagaaatcca
      961 cgacctgctg atcgacagcc ggaaactgat cgctcccgaa ggctgcctgt tttttgccct
     1021 gatcagcaaa cggaatgacg gacataaata cctcgaagac ctctacgacc agggagtacg
     1081 gaacttcgtg gttcagcggg cgccggaaaa ccttccgtca ttccccgaag ccaatatcat
     1141 ccgggttaag gacacgctaa gggctcttca gttactggca tcggaacaca ggaagcagtt
     1201 tgacatcccg gtgatcggca tcacgggaag caacgggaaa acgattgtca aagaatggct
     1261 gtaccagttg atgtcatcgg acaagaatat cgtccggagc cccagaagtt ataattccca
     1321 gatcggcgtt cccctgtcgg tatggctgat gaacccatcg aacgagatcg ccatctttga
     1381 agccggcatc tcggaaccgg atgaaatgga acatctgcaa gccgttatcc agcctacgat
     1441 agggatcttc accaacctgg gacacgctca cgacgaaaac ttcatcaacc ggttacagaa
     1501 atccggcgaa aagctcaacc tgttcaccca ggtggatacg ctcatctatt gcaccgatca
     1561 gaaagagctg caggaaggaa tcctgaaaac cggcctgttg agaaagataa acgtatttac
     1621 ctggagccgc catgaagatg ccgatttaaa aatcatccgt tgccagcata accaccattc
     1681 caccgaaatc agcggaattt acagagaaaa taccatttcg atatccatac cttttaccga
     1741 cgaagcttcg gccgataatg ccattcattg ctggtcagcc ctgctttacc tggggtatga
     1801 caacgaagtg atcgccgggc ggatgaaatc gttgcagcct gttgccatgc gcctcgaaat
     1861 gaaggagggg atcaataact gtacgctgat caacgacagc tacagcaacg acatcgactc
     1921 actttcgata gctcttgact tcttaaacca acaaaaacag cacaaaaaaa agaccgcaat
     1981 actttcggat atccttcaaa gcggccgcag cgaccggtat ttatataacg agattggtaa
     2041 actgctggcc cgtaaagatg tggaacaggt gatcggaatc ggtgaagcca tcagcaaaca
     2101 gtcgggaagt ttcagcatga aaaaggcgtt tttcaaggat actgatgact tcctgaattc
     2161 atactccttt tcatcgttct caaacgagtg catcctggtg aaaggggcca gggtctttga
     2221 atttgagcgg atcattgaac agcttcagca aaaaacgcac gagacaattc ttgaaatcaa
     2281 cctgaatgcc gtggttcata acctgaacta ttacaggtcg aagcttaagc cgggagtgaa
     2341 actcatggcg atggtcaagg cattttcgta cggaagcgga agctatgaaa ttgccaatac
     2401 cctgcagttt caccaggccg attacctcac cgtggcatac gccgacgaag gcgttgaatt
     2461 aaggaaagcc gggattacgt tgcccattat ggttatgaac cccgacgagc agggatttga
     2521 ttcgatgttt aaatacggcc tcgaacccga agtttacagc atgcgcatcc tgaacatgct
     2581 caacaatgcc attcagcagc acaatccggc ccccggtccg gtcggcattc atgtgaaaat
     2641 agataccggc atgcaccggc ttggctttga tcccggagaa gttgatgatc tgatcaggaa
     2701 tctgaaagga aattcacata tagaggtacg ttccgtattt tcccatttgg ccggggccga
     2761 cgaccctgcg ctggacgatt ttactcatca ccagatcgaa acgctgaaaa agaccagtga
     2821 aaagattcag aaggcctttg attatcccat tctgcgtcat atcgacaata cggcaggcat
     2881 cacacggttc ccggaagcgc agtttgacat ggtccggctg ggtatcggcc tgtacgggat
     2941 cgcccctctt gccccggaac aggaaaacct gcaaaatgtc agcactttac aaaccgtcat
     3001 ctcacagata aagcacatcc cggccgacga tacagttgga tataaccgta aatggaaagc
     3061 caaaatcgat accaccatcg gaatcattcc gatcgggtat gccgacggat tgaaccgccg
     3121 gctgggcaac ggcgcaggaa aactcctggt caacggcaat atcgttccga ttgtcggcaa
     3181 tatatgcatg gatatgtgca tggttgatat taccggaatt gatgccgccg aaggcgacga
     3241 agtaattgtt ttcggcgccg gaatgccgat cactgaatta tccggtgcca taggaaccat
     3301 tccttacgaa cttctgacag ccatttcacg ccgggttaaa agaatatatt tcgaagaatg
     3361 atgcgatttt aacgatggaa ctttcactcg cgacaggaag aaaaggaaaa aaaacctccg
     3421 tctctttcat cacatgcgtt atcctgatcc tggccattgt cattttccgt tcgctgtatc
     3481 ctcccgttaa tatccttacc tgggatgtct tcggctatta tctttacctt cccgcccaat
     3541 tcatattcca tgatatcggg ttcaggaacc tggaatgggt caggcaaatt gccgaacaat
     3601 accagacaac ctctacgctc taccagcttg ttacctcccc cgacggagga ttcgtgataa
     3661 aatacagtgc cggcatggct gtattgaatg cacccgcttt ttttatcgct cacctgctgg
     3721 ctcagccgct gggattcccc gctgacggat tctccctgcc ctaccagtat gcatgggcca
     3781 taaacgggtt gatcgtcacg cttgcaggca ttttcatcct ccggaaaatt ttgctggaat
     3841 tctttgacga aactgtcgcc accatcgtcc ttgttatcgt tacgttagct accaattatt
     3901 tccaactgac tgctttcgat ggttacctta cccacaatta tacttttacc ctttgcgccc
     3961 tgattacctg gcttaccatc aagtggcata agaggccaac gtacccggca gcttgctggc
     4021 tcggcatttc gcttgggttg ataatcctgg tcagaccgtc ggaaatggtt tgcctgctca
     4081 ttcctgtact ttggggagta tattcccggg cttcagtaaa ggaaaagctg ctactggtac
     4141 gtgaacaccc ggggcaggtg ctggcactca ttttagccgt atttatagca ggattgcccc
     4201 aactgatata ttggaaaacc gttacggggc atttcatttt ttacagttac gtgaatgccg
     4261 gtgaaggttt cgattttgca aatccccata cctggcaggt attgttcagt taccgaaaag
     4321 gctggctggt ttatacaccc gtgatgttca tggccatcat cggctttgtt tccctgtacc
     4381 gtcataaccg gaaactgctc atcgctgtaa tggtctattt aattgtaaac ctgtacgtgg
     4441 taagcagttg gactacctgg tggtatgccg gtggcagtta cagccagcgt gccttgctga
     4501 catcctgtgt tttgatggcc cttccgttgg gctatttcat ccgggatatg ttcctccgga
     4561 aatggagcat ggcggttgtg atcctgatgc tgtcattaac attattactc aacctgtttc
     4621 aaacctggca atgggttcac ggcatcatcg acaagaccag gatgacccgt tcctattact
     4681 gggccgtttt cggaaaaaca accgtccccg aaaacgcatc cggactgctg cttattgaca
     4741 gatcggaaga ggattatctg aaaaatccgg aaaaatacag atcccgtatc ctgaagtttt
     4801 acgattttga aagttcaggc ccggaaaaag ggaactcctg cggtgaaaat tccttttcag
     4861 gaagttgcgc cgtgaaactc gattccacca ttaggttttc tcctgctgtg gaagcaacct
     4921 tcaggcagct taccgcggca gaccatgcct ggatcaggat aagcgccgaa gtattcccgc
     4981 ttaccgatcc ggctgaaaac ccggcatcac tggtagccac atttgagcat aacggcgaag
     5041 cctataaata tcaggccgaa gggattgaac ttgagaagcc tgcgctaaaa acaggccaat
     5101 ggaaccttgt ccggttcgat taccttaccc cggagatacg atcggagaac gataaactaa
     5161 aagtctattt ctggttacgc ggcaataaac cgatcctgat cgacgacctg aaagtggaag
     5221 tctgggagcc gaaataacgc cggacgctga cgcagccagt gaagatgctt aggcctgttt
     5281 ccggcgtata gatcatgtga gtaaggaagg atactattcc attcttggag tgaggccgga
     5341 tcggaccagg gaagtattat cagtagtcaa ttttccatca gaaagtg
//
LOCUS       DAOPYV010000114         5207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_20100_length_5207_cov_12.623641, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000114 DAOPYV010000000
VERSION     DAOPYV010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20100_length_5207_cov_12.623641"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PKI35_11175"
     CDS             <1..648
                     /locus_tag="PKI35_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX44988.1"
                     /translation="EVKVHAVNDVTLDFAEAEFAAIVGPSGSGKTTLLNLIGGLDKPT
                     SGEIMINGTDLSTLKSSELIDFRLNNIGFVFQSYNLIPVLTAKENVEFIMTLQKWPEK
                     ERDERTLELLNAVGLGDRINSRPSKLSGGQQQRVAVARALASKPKFVLADEPTANLDS
                     KSAVTLLEIMEKLNHESKITFIFSTHDPRVVKMAHRVITLEDGKVISDDIRELPS"
     gene            662..1810
                     /locus_tag="PKI35_11180"
     CDS             662..1810
                     /locus_tag="PKI35_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44989.1"
                     /translation="MKRFFVFVLFLLFFHEALHSQDTLSVSLSGYLTTMQSSMFDSLS
                     GPFMNENMLHNRLNFKAYIGSNITLSAELRNRLFTGDFVRQGKAYADIIGTDNGRVDM
                     SWNVVEEPSFLLNTTVDRLWADLRLGKFQATAGRQRINWGQALVWNPNDIFNTYSFFE
                     IDYPERPGSDAVRLQYFPSSSSAVEFAVKAGNDKKITAAGLYRFNRWGYDIQLLAGVS
                     GEEDIVIGTGWSGAIKSCSFRGEASWFAPRRDITGDESKVLATAGFDKVFSDNSTAQV
                     QILYCNHPLDPDNFDIFYSGSLSPKDLAFSEFSAFGQFTWAATPLLNLSIPAMWFPDL
                     EGYFAGPTMDYSLAENLDFSLIWQHFNAVMTGSRKNINMGYIRLKYSF"
     gene            2144..2548
                     /locus_tag="PKI35_11185"
     CDS             2144..2548
                     /locus_tag="PKI35_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44990.1"
                     /translation="MKKNILISLILLVLCVGCIQKSKAPEENKKIENSLKSISFLYDS
                     VGINPSELLYLFDRVNKAIDSIGYPDAGYKLWLIQGDSIKDLRFMVEGCWPDQAVYDS
                     IHNNVLYKNAWGNEKIFKKMKTISYHRFVLVR"
     gene            complement(2990..4168)
                     /locus_tag="PKI35_11190"
     CDS             complement(2990..4168)
                     /locus_tag="PKI35_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNX44991.1"
                     /translation="MKKPVASAFFNKLFSGICACLIVFTLFSCEKEKDDPQIETTSLT
                     GLSLSKYFVKAKITDKGDYKINDYGFDYYVGTSEGSYYNYGSVSLGSSIKSDTFSTVL
                     DLGQLSYYYNNYRCYARAYITNEKGTVYGKFISTDLVVLKLESIYPTKGKSGDTITLL
                     GKYFNPNPVLNRVYFNNTSADVISGDAQSLRVIVPQNISYYYNDGITVKVSSMGIESS
                     LSNAFKLLPTPLGFSPNYGNWSTSITINGSGMYDASVYFDDVYIYTNSYSSGSVSVSI
                     PYSFLKKKFKIYLSTDGVKTEVPGGYFTMDELFVYQLSNTQFSRGSTINFTGKGFNPE
                     ESYTKLILGNTVINASYCYSTYANFDIPYSMALGTYVTKITNSVDTLTLPYSLTIVGK
                     "
     gene            complement(4201..4947)
                     /locus_tag="PKI35_11195"
     CDS             complement(4201..4947)
                     /locus_tag="PKI35_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44992.1"
                     /translation="MNKIIPIILAVILITATLPAISQNSPAHFIKGWGTELNFNPFDG
                     SLSLNNATGQIKVRRFLNNDIALRAGITISAKNNTDKTKQAYGTSPYDASVKSKSNLV
                     ALSLGAEKHFNSGHRLSPYLGFEIGTGLKKTEQDFEYNHVTRTIKGAWMTEDVTYNGQ
                     YYVINDTYVERGFMSANANLFTGFDFYMADCFYFGYELGFGFEYTKYSKIEITQDPEY
                     PYSGLYPDFDSKSWSLGPRLLNGIRIGYNF"
BASE COUNT         1441 a         1116 c         1189 g         1461 t
ORIGIN      
        1 gaggtaaaag ttcacgcagt gaatgatgta acgcttgact ttgcggaagc tgaatttgcc
       61 gccatcgtcg ggccttcagg ctcaggaaag accacgctgc tgaacctcat cggcggactc
      121 gacaaaccta cttccggcga gatcatgatt aacggcaccg acctttcgac cttaaaatct
      181 tcggagctca tcgatttcag gctgaataac atcgggtttg tattccagtc gtataacctg
      241 atacccgtac tgactgcgaa agaaaatgtg gagtttatca tgacgcttca aaaatggccg
      301 gagaaagaga gggatgaaag gactcttgag ttgctgaatg cggtgggcct gggcgaccgg
      361 ataaacagcc ggccttcgaa attgtcgggg ggacagcagc aaagagttgc cgttgcaagg
      421 gcgcttgcct caaaaccgaa atttgtcctt gccgatgagc ctacggccaa cctcgattca
      481 aaatcggccg ttacgctgct cgaaatcatg gaaaagctga atcatgaatc gaaaattacc
      541 ttcattttct caacccatga tccccgggtg gtgaagatgg cgcatcgcgt catcaccctc
      601 gaagacggaa aagtaatttc ggatgatatc agggaactcc cttcctaata atcctgtcct
      661 gatgaaaaga tttttcgttt ttgtattgtt tttactcttc tttcatgaag cgctgcattc
      721 gcaggatacg ctttcggtat cgctgagcgg ctatctcaca acgatgcaga gcagcatgtt
      781 tgattccctg tcggggccgt tcatgaatga aaacatgctg cacaaccggc tgaacttcaa
      841 agcgtatatc ggcagcaaca tcaccctctc cgccgagtta aggaaccggt tgtttaccgg
      901 ggattttgtc agacagggaa aagcgtatgc cgacatcatc gggacagata acggccgggt
      961 tgacatgtcg tggaatgttg ttgaggaacc gtcgttcctc ctcaatacaa ccgtcgacag
     1021 gctttgggcc gatctgcgtc tcgggaaatt ccaggcgaca gccggaaggc agcgtatcaa
     1081 ctggggacag gccctggtat ggaaccccaa tgacattttc aacacttatt cctttttcga
     1141 aatcgattat cctgagcggc ccggaagcga tgccgtcaga ttgcagtatt tcccttcatc
     1201 ctcctctgcc gtggaatttg ccgtgaaagc cggcaatgat aaaaagatta cggcggcagg
     1261 actttacagg ttcaaccggt ggggatacga tatccagctc ctggcaggag tctccggaga
     1321 agaagatatc gtgataggga ccggctggtc gggagcgatt aagagttgct ccttcagggg
     1381 cgaagcctcg tggtttgctc ctcgcaggga tattacggga gatgaaagca aggtgttggc
     1441 gactgccggt ttcgataagg tgttttcgga caattccaca gcccaggtac agatcctgta
     1501 ttgcaaccat ccgcttgatc cggacaattt tgatattttt tattccggga gcctctcccc
     1561 gaaagatctg gcattttcgg aattttcagc cttcggccaa ttcacctggg cggcaacgcc
     1621 cctgttgaat ctcagtatcc cggctatgtg gtttcccgac ctggaagggt atttcgcagg
     1681 tcccaccatg gactattccc tggccgaaaa tcttgatttc tctttgatct ggcagcattt
     1741 caatgccgtg atgacaggat cccggaaaaa cattaatatg ggatatatca ggcttaaata
     1801 tagtttctga cgcaaacgga actgtttgtt ccgaacattt tctcttcgcc ggaaattaat
     1861 ttataaaagc aatcaggcaa cgctgtcttg aagatcatct ttaaaagacc ctgaaggttt
     1921 tgaaaacctt cagggtctta accggcagac gcgccttctc cctatacaac tcctgcttac
     1981 atcggagcgg tcggatcatt gcagaaacaa aaatttatcc tgtgaagcat ctgcttgcaa
     2041 tctgccggac catttttaaa atatatttga tttaaatggt taaatatcat acctttaaaa
     2101 caggattaac taaaccagac attaaccttc aaatccatta aaaatgaaaa aaaacattct
     2161 tattagtttg attcttctgg tattgtgtgt tggttgtata caaaaatcaa aagcgccgga
     2221 agagaataaa aaaatagaaa actcattgaa gagtatctct tttctgtatg actcggttgg
     2281 aataaatccg tctgaactgc tgtacctctt tgatcgggtt aataaagcca tcgattccat
     2341 aggctatcct gatgcagggt ataaattatg gctcatacag ggtgattcga tcaaggatct
     2401 cagatttatg gttgagggat gttggcctga tcaggccgtt tatgactcaa ttcataacaa
     2461 cgtcctgtat aaaaatgcat gggggaatga aaaaatcttt aaaaagatga aaacgatctc
     2521 ctatcaccgg tttgtgcttg tcaggtaaga agggtttcct gcattaatat tcaccgctcc
     2581 ggctcgcatg ctttctgacg cgtgcgctaa gattcattat gccgtttaaa atttgtttgc
     2641 tcttattgca tgattagaaa tataacatac cagaagaatg agtgtatgag aagaatgccg
     2701 cacacgccgg gagttgcgcg agccaggggc aactcgaaag accctgaagg ttttgaaaac
     2761 cttcagggtc tgatcggccg gcaggccttc tccctgagcg cctgtcccga ttcatcggga
     2821 attaggcgtg gcaggcggat gaggcgaaag catgcgttat tacagcagca aaacgaaata
     2881 actgtccggt aaccggttca tacctgaaaa gccggacttt atcaaaacaa tcctgttccc
     2941 tttgggtaat aacccgaata aggtgatgca ggctgcccgg atatatgcat catttcccaa
     3001 cgatcgtgag cgaatagggc aatgtaaggg tgtccacact gttggttatt ttggtcacat
     3061 aggtacccag ggccatactg tacggaatat caaagttggc ataggtacta taacaatagc
     3121 tggcattaat taccgtattt cctaatatca gcttggtgta ggattcttcc gggttgaatc
     3181 ctttaccggt aaagttgatg gtgctccccc tgctgaattg ggtattacta agctgataaa
     3241 caaacaactc gtccatggta aaatagccgc ccgggacttc tgtctttacg ccatccgtac
     3301 tcaggtaaat tttaaatttc ttcttcagaa aactatacgg gattgacacg gaaaccgaac
     3361 cggaagaata tgagttcgtg taaatgtaaa catcatcgaa atatacggaa gcatcgtaca
     3421 taccggaacc attaatggta atactggtac tccagttgcc gtaatttggt gaaaatccca
     3481 acggggtggg taatagctta aacgcattcg acagagagga ttcaatcccc atggaagata
     3541 ctttaacggt aataccatca ttgtaataat aagaaatgtt ttgaggcaca ataactctca
     3601 gtgattgagc atcgcctgaa atcacatctg cgctggtatt attaaaatag accctgttta
     3661 agacagggtt cgggttaaaa tactttccga gcaatgtaat agtatcaccg cttttgcctt
     3721 tggtcgggta gatgctctcc agtttcagta caaccaggtc ggtggaaatg aatttaccgt
     3781 acacagtacc cttttcgttt gttatatagg ccctggcata gcaacgataa ttgttgtagt
     3841 aatagctgag ctggcccaga tcgagtacgg ttgaaaaggt atcgcttttt attgaactgc
     3901 ctagtgaaac acttccataa ttgtagtagg agccttcgct ggtacctaca taataatcga
     3961 atccgtaatc attgatttta taatcacctt tatccgttat tttagcctta acaaaatact
     4021 ttgataggga gagaccggtg agggatgttg tttcaatctg agggtcgtct ttttcttttt
     4081 cacacgaaaa cagcgtaaaa acgatgaggc aggcacaaat tcctgaaaat agcttgttga
     4141 aaaatgccga agcaactggt tttttcatcg taataaattt ttaagattta aggttgaata
     4201 ctaaaaatta tagccgattc tgataccatt cagtaaacga gggcctaaac tccagctttt
     4261 tgagtcaaag tcagggtaaa gccccgaata gggatattcc ggatcctgcg taatttcaat
     4321 tttcgaatat ttggtatact caaatccgaa acccagttca tatccgaagt aaaaacaatc
     4381 ggccatgtaa aaatcaaagc cggtgaacag gttagcgttg gcactcatga accctctttc
     4441 aacgtaagta tcgttgataa cataatactg gccgttatac gttacatctt ccgtcatcca
     4501 tgcgccttta atggttcttg ttacgtggtt atattcaaaa tcctgttcgg tttttttcaa
     4561 accagtcccg atttcgaagc ctaaataagg cgataagcgg tgtccgctgt taaaatgctt
     4621 ttcagcgcca agactgagcg ctacaaggtt cgatttactc ttaacgcttg catcataagg
     4681 tgatgttccg taagcctgtt tcgtcttgtc agtattattc ttcgcagaga tagtaattcc
     4741 ggcacgcaag gcaatatcat tgttcaggaa cctgcggacc tttatttgtc ctgttgcgtt
     4801 attaaggcta aggctgccgt caaaagggtt gaaattaagt tccgtacccc agcctttaat
     4861 aaagtgggcc ggcgaattct ggctaatggc cggaagagta gctgtaataa ggataacagc
     4921 aagaataatc ggaatgattt tgttcatggt tcaaaagagt taaggatgat taaaatttat
     4981 gtattaggtg aaattgattc atggaaaaca gagacaaata tattgaaatt attttattgt
     5041 gtgttttgat tttcttcttc tgttctgctt gcgcttttcc ggatgcatgc gctgatttta
     5101 tcatgctgtc aatatttttt tgatagaccc tgaaggtttt gaaaaccttc agggtctgct
     5161 acgccggcag gccttctccc tgagcgcctg tcccgattca tcgggaa
//
LOCUS       DAOPYV010000115         5056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_20934_length_5056_cov_11.426515, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000115 DAOPYV010000000
VERSION     DAOPYV010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20934_length_5056_cov_11.426515"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PKI35_11200"
     CDS             complement(<1..253)
                     /locus_tag="PKI35_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44993.1"
                     /translation="MINAIKKIPYSRWDSKNKWWTIPYSVQFTEQLTEVCKSQGLIIS
                     FEEELSDITEVKRISRYDIPNYREVPEEYILKIKELRYSN"
     gene            complement(501..707)
                     /locus_tag="PKI35_11205"
     CDS             complement(501..707)
                     /locus_tag="PKI35_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNX44994.1"
                     /translation="MKANIGKSDKLIRLLLALIGVLLYVFNVVSGTFGIVILVIAALL
                     IFTSLINYCPLYTLFGINTCKKEK"
     gene            890..1447
                     /locus_tag="PKI35_11210"
     CDS             890..1447
                     /locus_tag="PKI35_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNX44995.1"
                     /translation="MELFTIFLIAVSLSFDTFAVSISSGLALPKINLRNAVRIAGILA
                     FFQALMPVAGWLAGKGFSELIKDYDHWIAFILLVILGGKMIYESLVNGTEERNFNPLD
                     LKVCILMAIATSIDALVVGVSFAFLEFRIVVSTLMIGLVTFVVSLLGIFFGRKVGARL
                     GKRMEIVGGLMLVLIGLKILLEHLA"
     gene            complement(1570..1779)
                     /locus_tag="PKI35_11215"
     CDS             complement(1570..1779)
                     /locus_tag="PKI35_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX44996.1"
                     /translation="MVTFRPIRRYQSMVVSSPLLVKGNAYTIYKGGSTTGADDGGNYT
                     GGAYTPGTLVKAFTVTGAVTSLSNL"
     gene            1911..2237
                     /locus_tag="PKI35_11220"
     CDS             1911..2237
                     /locus_tag="PKI35_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNX44997.1"
                     /translation="MMEVTYDDNHVKLARFAKAMGHPARVKILEILLKQSCCYSGNIA
                     EEFPIALSTLSQHLKELKDSGLIQGEINPPRIKYCINKENWELAKQLFNAFLIEKPVL
                     TKSCNG"
     gene            2230..3270
                     /locus_tag="PKI35_11225"
     CDS             2230..3270
                     /locus_tag="PKI35_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNX44998.1"
                     /translation="MAEKRLSLSLPGFSRNLLWLLLPAWYLLYRNLAPISDKLVYGIM
                     NLEKGDHFADSLRFFVFEVPKVLLLLTLIIFIVGIIRSYFTPERTRKALEGRSLFSGN
                     VLAAMLGIVTPFCSCSAIPLFLGFVETGIPLGVTFSFLIAAPMINEVAVVLLFGLFGW
                     KVALIYILTGLLIAITAGWIIGRLHLEKWVEGWVYQMKAGSHPEDEKLTFPARINMGL
                     SAVKEIVGKVWIYVIIGIAVGAGVHGYVPQDFMASFMGKSAWYSVPLSVLIGIPLYSN
                     AAGIIPIVSALLEKGAALGTSLAFMMAVIGLSLPEMIILRKVLKMPLILTFIGVVASG
                     ILAVGFLFNLIF"
     gene            3288..3524
                     /locus_tag="PKI35_11230"
     CDS             3288..3524
                     /locus_tag="PKI35_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNX44999.1"
                     /translation="MEIKILGTGCANCRNLEKATREAVAELDIDATVVKEEDIVKIMS
                     YGIMRTPALVVDEKVVLYGRVPSVSEIKEILTKK"
     gene            3542..3940
                     /locus_tag="PKI35_11235"
     CDS             3542..3940
                     /locus_tag="PKI35_11235"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNX45000.1"
                     /translation="MKRILILAILMIATGSLFAQKTAKLKIVYFHAEHRCPTCLSIEE
                     NTLKTLNTYFSKQFKSGEITFVSLDVSEEKNEQMVEKYEADGSSLFLTKVTGKKEKTT
                     DFTNFAFSYSRNKPEKFIAGLKAEIEKNLK"
     gene            3997..4695
                     /locus_tag="PKI35_11240"
     CDS             3997..4695
                     /locus_tag="PKI35_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HNX45001.1"
                     /translation="MEFLQNLIDQSTFPLLTAFLLGLMTAISPCPLATNITALAYISK
                     DVENRRTVFVNGLYYTLGRALTYTLIGAILFMGASKFHVSRTIQANGEKWIGPLLIVI
                     GILMLDFIKLPGSSGNLMQRFADKVRLGNGWGSLLLGLIFALAFCPYSGVLYFGMLVP
                     MALAQPTGMALFPVFAVATGLPVVLLAYLLAFSVGTVGKTYHKIKSFEIWFRRIVAVI
                     FILTGFYFIWIYFS"
     gene            4698..>5056
                     /locus_tag="PKI35_11245"
     CDS             4698..>5056
                     /locus_tag="PKI35_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNX45002.1"
                     /translation="MKEFFCNSGFEIAGILNLTGKQALQCIDNGALLVDVREEYEIAI
                     KDFNAPGKIFCPFSEFDSLAKTLPADRSLIVADCTGIHSKEAVRKLLDAGFPKVANLA
                     GGIAEWERDGLPMKQSA"
BASE COUNT         1291 a         1142 c         1221 g         1402 t
ORIGIN      
        1 tattgctgta ccgtaactct tttatcttca ggatgtactc ttcaggcact tcacggtaat
       61 taggtatatc atacctggat atcctcttta cttccgtgat atcagaaagt tcctcttcaa
      121 acgagatgat cagtccctga cttttacaaa cttccgtaag ctgctccgta aactggaccg
      181 agtacggtat ggtccaccat ttattcttcg aatcccagcg gctataaggt atcttcttta
      241 tagcatttat caacgcttat atcataaaat atacggaaga tatacagaag atatacaaat
      301 aatatagttt tatcatagat cagactgatg cagacccatt cctgaatgag gacaatgaat
      361 cagttttccg gcaacaggat tttatggccg gtttatgtcc tgatactgcc tgataacatc
      421 accctccagt tttgagtcag gtaacataca gcatcaggcc cgcacgttac ggattgccgc
      481 cggaagcgat catggctgtt tcatttctcc tttttacagg tattgatacc gaacagcgtg
      541 tacagcgggc aatagtttat gagagaggta aaaatcagca aggctgcaat aaccaggatc
      601 acgatgccga aagtgcctga aaccacgttg aagacgtaga gcaatacgcc aatcagggcg
      661 agcaacagcc ggataagttt gtcggatttt ccaatgttgg ctttcataag actagatatt
      721 ggttgaataa tagtttttct gtcatataaa gataatgaaa attcccgcaa tttcatccct
      781 ccggggcagc cattttataa ttagcggtat acgctttcct gaccgccggg attatttccg
      841 gaagggttat ccggaaatga ttctttactt ttgcaccaac atcttttgta tggaattatt
      901 cacaatattt ttgattgccg tcagtttgtc gttcgatacg tttgccgttt ccatatcgag
      961 cgggctggcg ttgccgaaga taaacttaag gaatgccgtt cgcatagccg gcatacttgc
     1021 ctttttccag gcgctgatgc cggtggcagg gtggctggcc ggaaaggggt tttcggaact
     1081 gataaaggat tacgatcact ggatcgcctt tatccttctg gtgatcctgg gcggtaaaat
     1141 gatctatgaa agcctggtca acggaacgga agaacgcaat tttaacccgc tggacctcaa
     1201 agtatgcata ctgatggcaa ttgccaccag catcgacgcg ctggtcgtag gggtgagttt
     1261 tgccttcctg gagttcagaa tcgtggtttc cacactcatg atcggactgg ttacctttgt
     1321 cgtttcgttg ctgggaatat tcttcggcag gaaagtcggc gcaaggctgg gaaagagaat
     1381 ggaaattgtt ggcgggctca tgctcgttct catcggactg aagatactcc tggaacattt
     1441 agcctgagac ggcttgatca accttttcct gacgaaagaa acggatcccg gaatctgtct
     1501 taacgaatta tggtttgcca gggcgccgat tttacaacat gcaatggttc aactaatttc
     1561 ccggctgttt taaaggttcg acaggctggt aactgcgcct gttacggtaa atgcttttac
     1621 cagcgttccg ggagtatagg cgccgccggt atagttacct ccatcatcag cgccggtcgt
     1681 gcttccgcct ttgtaaattg tataggcgtt tccttttacc agcaatggtg acgaaaccac
     1741 catcgactgg tacctgcgaa tgggccggaa tgttaccacg tcgtttatgg taatatatag
     1801 gctctcaagc cattcataag ctttgctgac ggcaatgtcg gttagtcaaa aaaaaatcgt
     1861 tttggtatat caaatcagat atttcgtatt tttgcgaaat acaaaataga atgatggaag
     1921 taacctatga cgataaccat gtgaagctgg cgagatttgc caaagccatg gggcatcctg
     1981 cgcgtgttaa aatacttgag attcttttga agcagagttg ctgttacagc ggaaacattg
     2041 ccgaagagtt tccgattgcg ctttccacgc tttcgcagca cctgaaagag ctcaaagact
     2101 cggggctgat acagggcgag atcaatccgc cccgtattaa atactgcatc aataaagaga
     2161 actgggagct tgcaaagcag cttttcaatg ctttcctcat cgaaaaaccc gtgttgacaa
     2221 aatcctgcaa tggctgaaaa acgactttcc ttgtccttgc ccggattcag caggaacctg
     2281 ctgtggcttt tgctgccggc atggtacctc ttatacagga acctggcccc gatctcggac
     2341 aagctcgttt atgggatcat gaatttggag aaaggggacc atttcgccga ttcgctgcgg
     2401 tttttcgtat tcgaggtgcc gaaggtgtta ctgttgctca cccttatcat cttcatcgta
     2461 ggcatcatac gatcctattt tacgcccgaa cgcacgcgaa aagcgctgga aggcaggtcg
     2521 cttttttcgg gcaatgtgct ggcagcgatg ctcggcatcg taacgccttt ctgctcatgt
     2581 tccgcaatcc ctttatttct gggtttcgtt gaaacgggga ttccgctggg cgttacattc
     2641 tcatttctga tagcggcgcc tatgatcaac gaagtggcgg tggtgttgct gtttggattg
     2701 ttcggatgga aggtcgccct tatatacata ttaacgggat tgctgatagc cataacggcg
     2761 ggctggatta tcggccggct tcatcttgaa aaatgggtcg aaggctgggt atatcaaatg
     2821 aaagcaggaa gtcatcccga agacgaaaaa cttacttttc ccgcccgcat caacatgggc
     2881 ctttctgcag taaaggaaat tgtcgggaag gtctggatat atgtgatcat cgggatagcg
     2941 gtaggggcgg gggttcatgg atatgttccc caggacttta tggcatcctt catgggcaaa
     3001 tcggcctggt attccgtccc gctttccgtc ctgatcggca ttccgctgta ttccaatgct
     3061 gcaggcatta tccccattgt ttcagccttg ctcgaaaagg gtgctgcgct tggcacatca
     3121 cttgcattca tgatggcggt cataggcttg tcattaccgg agatgatcat ccttcgaaaa
     3181 gtacttaaga tgccgttgat cctgactttt atcggggtag tggcttcagg aatcctggct
     3241 gtaggatttc tttttaacct gatcttttaa tcttaaaaac gcgaaatatg gaaataaaaa
     3301 ttttgggaac cggttgcgca aattgccgta acctggaaaa agcgacccgt gaagccgttg
     3361 cagaactgga tattgatgca acagtggtta aagaagaaga tatcgtgaaa ataatgagct
     3421 acggcataat gcgcacccct gcgctggtcg tggacgaaaa agtggtcctg tacggaaggg
     3481 tgccatcggt aagcgaaata aaggaaattt taacaaaaaa ataacctcca acttttaagt
     3541 catgaaaaga atactcatcc ttgccattct gatgattgct accgggtcat tgtttgccca
     3601 gaaaacggct aagctgaaga tcgtctattt ccacgctgaa catcgttgtc ctacctgtct
     3661 ttccatcgag gaaaataccc ttaaaaccct gaacacctat ttttcgaagc agtttaaatc
     3721 gggcgaaatc actttcgttt cgctggatgt ttccgaagag aagaatgagc agatggttga
     3781 aaaatacgaa gctgacggct catcgctatt ccttacgaag gttaccggga aaaaggaaaa
     3841 aaccaccgat tttacaaact tcgcctttag ctattcgcgc aacaagcctg aaaagtttat
     3901 tgccggactc aaagccgaaa tcgagaaaaa cctgaaataa tttggtgatg tttcaatttt
     3961 caaatcttca aattgtcaca ttttcaaatt agaaaaatgg agttcctgca aaacctaatc
     4021 gaccaaagca cttttccact gctcaccgct ttcctgctgg gtttgatgac agccatcagt
     4081 ccctgtccgt tagctaccaa tatcactgcc ctggcgtaca tcagcaaaga tgttgaaaac
     4141 cggcggacgg tctttgtcaa tggactttat tacaccctcg gcagggcact gacttatacc
     4201 ctgatcgggg cgatcctctt tatgggggcc agcaaatttc atgtaagccg caccatccag
     4261 gccaacggtg aaaaatggat aggcccgttg ctgattgtca ttggcatcct gatgctcgac
     4321 tttatcaaac ttcccgggtc atcgggaaac ctgatgcaaa ggtttgccga caaggtaaga
     4381 cttggtaacg gttggggatc gttgttgctg ggcctgattt ttgcccttgc tttctgccca
     4441 tacagcggcg tgctttactt cggaatgctt gttccgatgg cgctggcaca gccgacgggg
     4501 atggcgttat tcccggtatt tgccgtggct accgggcttc cggtagtatt gctggcgtat
     4561 ctgctggcct tcagcgtggg aaccgtaggc aaaacctacc ataagattaa atcttttgaa
     4621 atctggttcc gcaggattgt ggcggtaatc tttatcctta cggggtttta cttcatctgg
     4681 atatatttct cataaccatg aaagaattct tttgcaattc agggttcgaa atagccggca
     4741 tccttaacct gacgggcaag caggccttac aatgtattga taatggagca ttgctggttg
     4801 atgtgcgcga agagtatgag attgcgatca aggattttaa tgctcccgga aaaattttct
     4861 gccctttttc ggaatttgac agcctggcga aaacgctccc cgccgaccgg tcgctgatcg
     4921 tagccgactg cacggggatt cacagcaaag aagccgtccg taaactgctt gatgccgggt
     4981 tcccgaaagt agccaatctt gccggcggaa ttgccgaatg ggaacgcgac gggttgccga
     5041 tgaagcagag tgccga
//
LOCUS       DAOPYV010000116         4977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_21439_length_4977_cov_12.021130, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000116 DAOPYV010000000
VERSION     DAOPYV010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21439_length_4977_cov_12.021130"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            615..2246
                     /gene="hcp"
                     /locus_tag="PKI35_11250"
                     /gene_synonym="priS"
     CDS             615..2246
                     /gene="hcp"
                     /locus_tag="PKI35_11250"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNX45003.1"
                     /translation="MFCNQCQETAKNTGCTINGVCGKKEQTANIQDLLVYACQGLAFA
                     AIEARKAGIDTTAENRHITNSLFMTITNANFDDEAIIRAIRECTDYRDNLKKKVSIIE
                     KHDSLDWNGSTEEEFYEKATQVGTLTYDTNEDIRSLKQYTLFGVKGMAAYTEHAFNLG
                     FEDPDLYQFMQEALVMITKPMDLNGMLDWVMRTGEHGVKVMALLDKANTATFGNPEIS
                     KVNIGAGKNPGILVSGHDLKDLEELLIQTEGTGIDIYTHSEMLPSHAYPQFKKYKHLV
                     GNYGNAWHRQLEEFESFNGPVLFTTNCLVPPRNTSTYNDRIFTTGATGMPGWKHIDKK
                     LANGQKDFSEIIELARKCPPPAEIEKGEITIGFAHNQVLSLAGKILEAVNSGAIKKLV
                     VMSGCDARQKSREYYTEFAKQLPGDTVILTSGCAKYRYNKLNLGDINGIPRVLDAGQC
                     NDSYSWALVALKLKEVLNLDDINKLPIVFNIAWYEQKAVIVHLALLYLGIKNTHIGPT
                     LPGFMTPNVLNVVREKFGVQTITTVEDDLKVFGIN"
     gene            2736..4883
                     /locus_tag="PKI35_11255"
     CDS             2736..4883
                     /locus_tag="PKI35_11255"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNX45004.1"
                     /translation="MKKQILILLFCILNTLFAVAQEGMWMLSQLGQLDLPKKGLLIPA
                     EKVYSPDKPCLANAIVQLGGGTASFVSPEGLLVTNHHVAFTALQRSSSVSSDYLTNGF
                     LAKDRQAEIQAPGYQAKLMLEMNDVTPDVLASAKGLTDAVERDKKINAAIAKMTEAVK
                     QKGSDLEAEIAEMFNGKQYIMYTYKVFRDIRIVYSPPLSIGNYGGETDNWMWPRHTGD
                     FSFMRVYVSPDGKGNEYSADNVPFKPKVWLKPAQGDLDDGDFCFIMGYPGSTTRYRSS
                     NSVNWNLNYNYPFSVSNYKEIISLAEELTKKDPAGKIKTASLVKGLANVMKNYQGKVE
                     GMIKTDFLQKKLDFEKEYLKWANSTPERKAKYADILEKEKSQYNVLEKTRERDNVFGI
                     LNNLSGTMTGAAGQIYFISREMEKPAEERQPGITEEALKEFADALTYAYNDYYEPLDK
                     AMLLRAVKMAEALPADQRIAGLDNILANPGKTPEQWVDDAYSHTRLKDPEFVKSLIGK
                     SSADLENTGDPFIRLAAALYPMSEDINKVTEVFGANVTELRKDYLDALYEWKGSAMYP
                     DANSTIRFTWGPVKGYKPADAVWYYPFTSLQGVVEKNTGEEPFNAPAGLVSLEQKRDF
                     GRWADPELNDVPVAFLNQCDITGGNSGSPVMNAKGEIIGVAFDGNYEAMISDWQYDYA
                     LQRCIAVDIRYVLFVTEKFGNAGFLLEEMGVKK"
BASE COUNT         1460 a         1179 c         1124 g         1214 t
ORIGIN      
        1 atattgaatc tccatccgga tcaaaatcat tttttagcat gtttagcgta taagtctgcc
       61 cgacaaaacc ttttatggta tcgttaacag ctacgggcgc attgttctgc ccaaacgaaa
      121 cgctgataat ggcgaaaacc gcaagagatg atagtaaaat ctttttcatt ttaataaatt
      181 attggttaat acttgtgcaa atataatact aaaatctttt tttatttcat aatacaatta
      241 ttttttaact aaatattaac atttatcaac gtttgaagat caacttacaa atcgggcgac
      301 gcctcttgct cattgatttt ttgcaatgag gctttcaccg ggcatcattt gtaaaccagt
      361 taccctacat aatattcgtt tcgtcccaga atgtgtatta ttagtgatta aaatccgtca
      421 tctctccacc tgaacacagt caaaatttct ctcttaacgg aatatgcctt atgaatagct
      481 gaaaacccct ggctgttacg acaggcataa aagactcatt taacgataat taaaatattt
      541 ttattcatga gatacaattt acgtgtattt aagtatattt gtactttaat tcttaatcta
      601 aaaatcttat gagcatgttc tgtaatcaat gtcaggaaac agccaaaaac acaggctgta
      661 ccatcaacgg agtctgcggt aaaaaagaac aaaccgccaa cattcaggat ttactggtat
      721 atgcctgtca gggccttgcc tttgctgcga tcgaagcccg taaagcaggg attgatacca
      781 ctgctgaaaa caggcacatt accaacagcc tgtttatgac catcaccaat gccaattttg
      841 atgatgaagc catcatccgg gccatccggg agtgtacgga ttaccgggac aacctcaaaa
      901 agaaggtcag catcattgag aaacacgatt ctcttgactg gaatggttca acagaagaag
      961 aattctatga aaaggccact caggtcggaa cgttaaccta tgataccaac gaagatatcc
     1021 gctcgctgaa gcagtacacc ttatttggcg tcaaaggaat ggccgcatat acagaacatg
     1081 ctttcaacct gggttttgaa gatccggatc tctatcaatt catgcaggaa gcactggtaa
     1141 tgatcaccaa accgatggat ctgaacggta tgctggattg ggtgatgcgc acaggcgaac
     1201 acggggttaa agtcatggcg ttgctcgaca aggcaaatac ggcaacgttt ggcaatcctg
     1261 aaatatcaaa agtgaatatc ggggcaggaa agaacccggg catactggtc agcggccacg
     1321 acctgaagga cctggaagaa ttattgattc aaaccgaagg aaccggaata gatatctaca
     1381 cacattccga aatgctgcct tcgcatgcct atccccaatt caagaagtat aaacatctcg
     1441 tcggcaatta cggaaatgcc tggcaccgac aactcgaaga atttgaatcc tttaacgggc
     1501 cggttctctt tactaccaac tgcctggttc ctccgcgcaa tacaagcaca tataacgaca
     1561 gaatatttac gaccggggca accggaatgc cgggatggaa gcacatcgac aagaaactgg
     1621 ccaacgggca gaaagatttt tcggaaatca tcgaactggc ccgaaaatgc cccccacccg
     1681 ctgaaattga aaagggagag atcacgatcg gcttcgccca caaccaggta ttatcgcttg
     1741 ccggtaaaat tctcgaagcc gtgaattcag gggccattaa aaagctcgtt gtcatgtcgg
     1801 gctgcgatgc acgccagaaa agccgcgaat actataccga atttgcaaaa caactccccg
     1861 gggataccgt aatccttact tcaggatgcg cgaaataccg ttacaacaaa cttaaccttg
     1921 gcgatatcaa cggcatcccg agggtgctgg acgccggtca gtgcaatgat tcctattcct
     1981 gggcgctggt tgcgcttaag cttaaagaag tcctgaacct ggacgatatc aacaagttgc
     2041 cgattgtttt caatattgca tggtacgaac agaaggccgt catcgttcac cttgccttgc
     2101 tgtatctggg catcaagaat acccatatcg gccccacctt gccgggattc atgacgccca
     2161 acgtactgaa tgtggtccgt gaaaagtttg gcgttcagac catcaccacg gttgaagatg
     2221 atttaaaagt gttcggaatt aattaaaaaa caacgcgtaa caggcttcac ggtgagcgcg
     2281 gatcaaaccg atcctgcatg ctcaccgtga agttttttta gtgctgttaa aataagggga
     2341 cgatgccggt tatcccctcc tttgattttc aggatattcc cccattacta tctctgtacc
     2401 aacagaataa aaactcgcag agaacccgtt gagattcaca gaagatttga tgagattcac
     2461 cgagaaattt tgagacagcc tttctttgtc cttattttta tccgtaatca cagccgaagg
     2521 tttaaaccgc agacgttaat gaaaaccagg ttgaattaac taatatttcc ttcctgcgtc
     2581 aaaacaggga gcccggacca ttctgcggca attaccccgt ttgtctgcct catcaaactc
     2641 cttatatctc cttttctctc accctcacac ccataaccct tttacccggc tgatttgcaa
     2701 catttttcca cttccataaa accaaacctt taaccatgaa aaaacaaatc ctgattttat
     2761 tgttttgcat cctgaatacc ctcttcgctg ttgcccagga gggaatgtgg atgctttccc
     2821 agttaggaca actggatctg cccaaaaagg gactgttgat acctgcggag aaggtataca
     2881 gcccggataa gccgtgcctt gccaatgcca ttgttcagtt agggggagga accgcatcgt
     2941 ttgtttcccc cgaagggttg ctggtaacca accaccacgt ggcttttacg gcgctgcaac
     3001 gatcctcaag cgtaagcagt gattacctta ccaacggatt cctggccaaa gaccgccagg
     3061 ctgaaattca ggctcccggt taccaggcca aactgatgct ggagatgaat gatgtaaccc
     3121 cggatgtgct ggcttcggca aaaggtctta ccgacgctgt tgagagagat aagaaaataa
     3181 atgccgccat cgctaagatg acggaagctg taaagcaaaa aggcagcgac ctggaagctg
     3241 aaattgcaga aatgtttaac ggtaaacaat acatcatgta tacctataag gtcttcaggg
     3301 acatccgcat cgtctattcc ccgccgctgt cgatcggcaa ttacgggggc gagaccgata
     3361 actggatgtg gcccaggcat accggggatt tttccttcat gagggtatat gtcagccccg
     3421 acgggaaagg caatgaatac agcgccgata atgttccctt taagccgaaa gtatggctga
     3481 aaccggcaca gggcgacctt gacgacggtg atttctgttt catcatggga tatcccgggt
     3541 ctaccacccg gtatcgttcc tccaattccg ttaactggaa cctgaattat aattatcctt
     3601 tctccgtcag caattacaaa gagattattt cactggctga agagttgaca aaaaaagacc
     3661 ctgccggaaa aatcaagacc gccagcctgg tgaaagggct ggcgaatgtc atgaaaaatt
     3721 accagggaaa agttgaggga atgataaaga cggatttcct tcaaaagaag ctcgattttg
     3781 aaaaagaata cctgaaatgg gccaacagca cgcccgaacg gaaggctaaa tatgctgaca
     3841 tacttgaaaa ggaaaaatcc cagtataatg tgcttgaaaa gacccgggaa agggataatg
     3901 tgttcgggat tctgaacaac ctttcgggca ccatgacagg cgcagccggt cagatatact
     3961 ttatatcccg cgaaatggag aagcccgcgg aagaaaggca gcccggcatc accgaagaag
     4021 ccctgaagga atttgccgat gctctgacgt atgcttataa cgattactac gaacctttgg
     4081 ataaggcaat gctgttgcgt gccgttaaaa tggcagaagc cttgccggca gatcagcgca
     4141 ttgcgggtct ggacaatatc ctcgcaaacc ctggtaaaac cccggaacaa tgggtggatg
     4201 atgcatacag ccacaccagg ctgaaagatc cggagtttgt aaaaagcctg atcggaaaat
     4261 catctgccga tcttgaaaac accggcgatc cctttatccg gctggcagcc gccctgtacc
     4321 cgatgtcgga ggacatcaac aaagtcactg aagtatttgg cgccaatgtg accgaactga
     4381 gaaaggatta ccttgacgcc ctttacgagt ggaaaggttc cgcgatgtat cccgacgcca
     4441 acagcaccat ccgtttcacc tggggacccg tgaaaggcta taagccggca gatgccgtgt
     4501 ggtattaccc gtttacttcg ttgcagggtg ttgtggaaaa gaatacgggg gaagaaccgt
     4561 tcaacgcccc tgccggttta gtctcccttg aacaaaaacg cgatttcggc cgttgggccg
     4621 atccggaact gaacgatgtt cccgttgctt tcctcaacca gtgcgatatc accggtggca
     4681 acagcggcag ccctgtcatg aatgccaaag gtgaaattat cggcgtggcc ttcgacggca
     4741 actatgaagc gatgatcagc gactggcaat atgattatgc cctacagcgg tgcatcgccg
     4801 tagacatccg gtatgttctc ttcgttacgg agaagttcgg caacgccgga ttcctgctcg
     4861 aagagatggg agtaaaaaaa tgacggcgcc tgaaaccatc aagaatatcc cgggaaattc
     4921 ttgagggcac tgaaaaagta tctcttatta aattaatcct tagaaataag ttggcag
//
LOCUS       DAOPYV010000117         4850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_22271_length_4850_cov_11.241084, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000117 DAOPYV010000000
VERSION     DAOPYV010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22271_length_4850_cov_11.241084"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1529
                     /locus_tag="PKI35_11260"
     CDS             57..1529
                     /locus_tag="PKI35_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX45005.1"
                     /translation="MKILKYSIICLMLFSFASCEKFLDKAPLDSINTANFYQTKEDAI
                     AAINGAYQPLQWPKLYNMRIWTTDIWAGNSIVGAGGATDGVETQDIANFVTSTDNAAA
                     LDIWRGPAPGILRCNLVLENVPGMDIDQNLKDRILGEAYFLRANYYFILVRLFGDVPL
                     LTKAQTPDDDLRPYRTSSTVIYDTIVSDLKHAINLLPQRESYSGNDIGRASKGAAAGM
                     LAKVYLTLGNYAETVTLCQQVTALGYTLNENYADNFDPLKKNSQESLFEVQYYGKTTY
                     GFWDNENQASWLSTFTGPRNSDFVAGGYGWDQPTQEFMDQYEPGDKRKDITVLYEGCP
                     SYDGKDYKASYSTTGYNLRKFLVSKSITSISDGSPANWPVLRYADVLLMEAEALCELN
                     KLGEAYKPLNDVRQRAGLTGLSGLSQDDLRNAIRKERRLELAFEGSRWFDLIRYDNGQ
                     YAQNFFHSIGKTNFTSKFLLLPIPQKERDANPNLTQNSGY"
     gene            1547..2956
                     /locus_tag="PKI35_11265"
     CDS             1547..2956
                     /locus_tag="PKI35_11265"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HNX45006.1"
                     /translation="MKRYTCIAFCLSVLALVTGCKKDDLAELNKGNTPLELTLSNQSL
                     TLNEKQRDNVILTMNWTSGSNMGSNAAITYKLLIDKAGNNFSNPLTFDLGKTTYKYEF
                     TAKTLNALVRDSLGVEAGDEISLEAEVIATILSEDVEPQVSPLVSFKVTPYDPVSENL
                     YIYGNATEAGWDNTKAVALTPVEGEPGGFAWQGQLSAGDFEFLTVLGQDFPSYVKGNT
                     AAEIKLRTSASQPDNRWHADAPGVYKIAVNLLDLTISVTKMPGPDYSQIFMVGSAAPN
                     GWDISNATELVQNPDNLYEFTYTGVMNAGEFKFPVNRSSSWGQDMYMRLDDTHMYLHH
                     GGDPDDNKWTIDKKGYYTITLNLLDLTIKIDRLQLYMVGSATPIGWDISNAVQMTEDN
                     SNGCIFRYSGPMVAGEFKLPVNRNSDWGQDMYMKVDDTHMYRHHGGDSDDNKWTLTED
                     GNYTIVANVEDLTISIQKR"
     gene            3166..>4850
                     /locus_tag="PKI35_11270"
     CDS             3166..>4850
                     /locus_tag="PKI35_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 66 protein"
                     /protein_id="HNX45007.1"
                     /translation="MMSRYGKLLFLVVALAVSSCRKDAGTGSEGPVPGNPSAPAFYNL
                     AVATDKACYHPGEEIKFTLNGLTTFPVRVRYKHLNTLIADNVLPSAGWTWTAPADDFR
                     GYSVEVYDTVDHKEVIYGTTAVDVSSDWTKFPRYGFLSSYGVMSGEKIQQVIGELNRF
                     HINGLQFYDWHNKHHKPLPMTGGAPASSWKDIGNRDTYFNTVKGYIDAAHSRNMKTMF
                     YNLLYGAWDDAQDDGVDKQWYMYTDNTHTNIDIFALSSPFLSNLYLLDPSNPGWQQYL
                     MAQNDIIYRYLGFDGYHIDQLGERGSRYTYNGSFLDLSQAFGSFIQANNNAFPDKYAV
                     MNAVNQYGQQTIARSPVDFLYTEVWSPYDTYKDLASLIKQNNMLAGDDKNQVLAAYVN
                     YNLANTREYFNDASVLYADAVIFAFGGAHLELGEHMLCREYFPFNKLKMSDNLKVSLI
                     RYYDFMVAYQNLLRDGGDFNTIAIQSLDNKAKFANWPEGNGFVSVVGKQVGDRQIIHL
                     INFTASTTTSWRDNDGIQTMPPLISGAKISFTHPDTVNRIWVASPDIAGGSPQ"
BASE COUNT         1307 a         1227 c         1148 g         1168 t
ORIGIN      
        1 gaacaataag cgccggtgtt aacctgattt tctaagacac ttttaatacg aaagagatga
       61 aaatactgaa atattcgatt atttgcctga tgctgttttc gtttgcgtcc tgcgagaaat
      121 ttcttgacaa ggctcctctc gacagtatca acacggcaaa cttctatcag accaaagaag
      181 acgccatagc tgccatcaac ggggcatacc agcctttgca atggcccaag ctttacaaca
      241 tgcgtatatg gaccaccgat atctgggccg gcaacagtat tgtaggcgcc ggcggcgcca
      301 ctgatggcgt tgaaacccag gatatcgcca attttgtcac ttcaaccgat aatgccgccg
      361 ctcttgatat ctggagagga ccggctcccg gaatcctgag gtgtaacctg gtactcgaaa
      421 acgttccggg aatggacatt gaccagaatc tgaaagaccg tattctcggc gaagcatact
      481 tcctcagggc taactactat ttcatccttg tcagattgtt tggcgatgtc ccgttgctca
      541 caaaagctca gactcccgat gacgacctca ggccttaccg cacctcttcc acagttatct
      601 acgacaccat cgtcagcgac ctgaaacatg ccatcaacct gctcccgcag cgcgaaagct
      661 attcaggtaa cgatatcgga agagccagca agggcgctgc cgccggaatg ttggccaagg
      721 tatatcttac cctgggcaat tacgctgaaa cggttactct ttgtcagcag gttaccgcat
      781 tgggctatac cctgaacgaa aattatgccg ataacttcga tcctttgaag aaaaactcac
      841 aggagagcct gtttgaagtt cagtattacg gtaaaaccac ttatggtttc tgggataatg
      901 aaaaccaggc aagctggtta agcaccttta caggcccgcg taattccgat ttcgtagccg
      961 gcggctatgg ctgggaccag ccgacccagg aattcatgga tcagtatgaa ccgggcgata
     1021 aaaggaaaga tataaccgtt ttgtacgaag gttgtccatc ctatgatggg aaagattata
     1081 aagcttctta ctccacaacc ggttataacc tgcgcaaatt cctggtttca aaatcgataa
     1141 cctccatttc tgacggaagc cctgccaact ggcccgtact gcgttatgcc gacgtgctgc
     1201 tgatggaagc tgaagccctt tgtgaactca acaagcttgg cgaagcctat aaaccgctta
     1261 acgacgtgcg ccagagagcc ggcctgaccg ggctgagcgg cctttcacag gatgatcttc
     1321 gcaacgccat caggaaagaa aggcgccttg aacttgcttt cgaaggttca cgctggttcg
     1381 acctgatccg ttacgacaat ggtcagtatg ctcaaaactt cttccacagc atcgggaaaa
     1441 ccaattttac atccaaattc cttttgctgc cgattcccca gaaggagaga gatgccaatc
     1501 ccaaccttac ccagaattca ggttactaaa ccttaaatta actgatatga aacgatatac
     1561 atgtatagca ttttgcctgt cggtactggc tttggtgact ggctgcaaaa aagatgatct
     1621 tgccgaactc aataaaggca acaccccgct ggaacttacc ctttccaatc aatcgctgac
     1681 gctcaacgaa aagcaacgcg ataatgttat cctgacaatg aactggactt cgggaagcaa
     1741 catgggatcg aatgccgcca tcacgtacaa actcctgatt gacaaggccg gcaacaattt
     1801 cagtaacccc ctgactttcg accttggtaa aactacttac aaatatgagt ttacagccaa
     1861 gacgctgaac gcacttgtaa gggattcttt aggcgtcgag gccggtgacg aaatttcact
     1921 tgaggccgaa gtaatcgcta cgatcctttc ggaggacgtg gaaccgcagg tttcgcctct
     1981 cgtaagtttt aaggttaccc cttacgatcc ggtttccgaa aacctttata tttatggaaa
     2041 tgcaaccgaa gcaggatggg acaatacaaa agcggttgcc cttacccctg tggaaggtga
     2101 acccggagga ttcgcatggc agggacagtt gagcgccggc gattttgaat ttctgacagt
     2161 actcggtcag gattttcctt cctacgttaa aggaaacacc gctgctgaaa tcaaactgcg
     2221 gacttccgct tcccagcccg ataaccgttg gcacgccgac gcccccggtg tatacaaaat
     2281 agctgttaac ctcctcgatc tgaccatctc cgtcaccaaa atgccgggac ctgattacag
     2341 ccagatattc atggttggaa gcgccgcccc caatggctgg gatatcagca acgcaaccga
     2401 actggtacag aaccccgaca acctgtatga atttacctat accggtgtaa tgaatgccgg
     2461 agaattcaag ttcccggtca acaggagttc aagctggggc caggatatgt acatgcgtct
     2521 tgatgataca catatgtatc tgcaccatgg cggcgatccg gatgacaaca aatggaccat
     2581 cgataagaaa ggatattata ccattaccct gaacctgctc gacctgacca tcaaaattga
     2641 caggcttcag ttgtacatgg tcggcagcgc tacacccatc ggatgggata tcagcaatgc
     2701 cgtccagatg acagaggaca acagtaatgg ttgtattttc agatactccg gacctatggt
     2761 tgccggcgaa ttcaaactgc ccgtcaaccg taactccgac tgggggcagg atatgtatat
     2821 gaaggtggac gacacccaca tgtaccggca tcacggcggc gattccgacg acaacaaatg
     2881 gacgctgacc gaggatggca attacacaat cgtggctaat gttgaggatc tgaccatcag
     2941 cattcagaaa cgataggtta ggttgtttgg cttaagaatt cagaattcag attaagaagc
     3001 agccggaagc gatagttttc agttatatct gagattgata agggttagtt tcacgccctg
     3061 gctgcttcaa tctgaattct gattcttttt ctgacgttcc tggccctgga aatcctgagg
     3121 ccttcaactt ccgataattc ctctttgaat ttttcagcat acaacatgat gtcccggtac
     3181 ggtaaacttt tatttctggt agtggcgctg gctgtaagca gttgcaggaa agatgccggt
     3241 acaggcagcg aagggccggt tccgggaaac ccttccgcgc ctgccttcta taatctggcg
     3301 gttgcgacgg ataaagcctg ttatcatccc ggggaggaaa taaaatttac actgaacgga
     3361 ctgacaactt tcccggtcag ggtcaggtat aagcacctca atacgctgat agccgacaat
     3421 gtattgcctt cagccggctg gacatggact gccccggcgg atgatttcag gggctattcg
     3481 gttgaagtgt acgatacggt tgatcacaag gaagtgattt acggaacaac agccgtggac
     3541 gtttcgtcgg actggactaa atttccccgt tacggatttc tgagcagtta tggtgtgatg
     3601 agcggggaga aaattcaaca ggtaataggg gaactgaacc ggtttcacat caacgggctg
     3661 cagttttacg actggcacaa caaacatcat aaaccgcttc ccatgacagg cggtgccccg
     3721 gcatcttcct ggaaagatat cgggaacagg gatacttact ttaatacggt aaagggctat
     3781 atcgatgccg cccattcccg caacatgaaa accatgtttt acaacctgct gtacggcgcc
     3841 tgggatgatg cccaggacga cggggttgac aaacaatggt atatgtacac cgataacacg
     3901 catacgaaca ttgatatctt tgcgctctcc agtccgttcc tgagcaatct ttaccttctc
     3961 gatccgtcga atcccggctg gcagcaatat ctgatggccc agaatgacat tatttaccgt
     4021 tacctcggtt tcgacggcta tcatatcgac caactcggcg aaagaggctc ccgatatacg
     4081 tataacggat cattccttga tctttcacag gcttttggct cctttatcca ggcgaataac
     4141 aatgcctttc ctgataaata cgcggtgatg aacgccgtaa accaatacgg tcagcaaacg
     4201 attgcccggt cgcccgttga ctttttgtac accgaagtct ggtcgccata cgatacctat
     4261 aaggacctgg catcgcttat aaagcagaac aacatgctgg ccggtgacga caaaaatcag
     4321 gtgctggccg cttatgtgaa ttataatctt gccaatacca gggaatactt caacgatgcc
     4381 tccgtattgt atgccgatgc agtgatcttt gccttcggcg gcgcccacct cgaactgggg
     4441 gaacacatgc tttgccggga gtatttcccg ttcaacaaac tgaaaatgag tgacaacctg
     4501 aaggtttctc tcatccggta ttacgatttt atggttgcgt atcaaaacct gctccgcgac
     4561 ggaggggatt tcaataccat cgccatacag tcgcttgaca ataaggcaaa atttgccaac
     4621 tggcccgaag gcaacggatt tgtttccgtc gtagggaagc aggttggcga caggcagata
     4681 atccacctga tcaattttac cgcttcaaca acaacctcct ggagggataa cgatgggatc
     4741 caaacaatgc cgccgctgat cagcggggca aaaatatcgt tcacgcaccc ggataccgtt
     4801 aacaggattt gggttgcatc tcccgatatt gcaggcggct cgccgcaaga
//
LOCUS       DAOPYV010000118         4828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_22397_length_4828_cov_11.758014, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000118 DAOPYV010000000
VERSION     DAOPYV010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22397_length_4828_cov_11.758014"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="PKI35_11275"
     CDS             complement(<1..1183)
                     /locus_tag="PKI35_11275"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNX45008.1"
                     /translation="MKNTYFSNRQYVIIGVFLLAGIIFIFRLFYIQVIDKSYSLSANN
                     NVLRYVTQYPGRGLIYDRKGRLLVYNEAVYDLMVTPNQVKNIDTMELCRLIGIDYDGF
                     LERIEKARVYSPYKASPFEKQISKETYGYLEEKLYKFQGFYVQPRTMRQYPKPIAAHT
                     LGYIGEATQGVLDKDPYYKSGDYIGISGIEKSYEKELRGQKGMKIRMVDVFNREMGSF
                     QNGKYDTAAVMGQDLYTSLDADLQTYGELLMKGKRGSIVAIDPATGEILAFLSSPTYD
                     PNLLIGRTRGENYLKLNEDPVKPLLNRAIMGQYPPGSTFKIVNDLIGLQEGTLTPETY
                     YSCQGPTTSPIKCTHHHMSPLDLKEAIKQSCNPYHWQVFRAILQKHSYGSIKNAYDVW
                     YR"
     gene            complement(1256..1750)
                     /locus_tag="PKI35_11280"
     CDS             complement(1256..1750)
                     /locus_tag="PKI35_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX45009.1"
                     /translation="MKFYSKIVFFIIGGLSLTFFSCSKPEAYPVIPFISYQGFTLLLN
                     EDSITERGILQFSFTDGDGDIGLTQADTLPPYDFNLYITYFEKQNGVYKKVDLPSTLN
                     ARIPVLTPSGNIKSIKGDIQDTLYVNNPASTFDTIKFEVYIVDRALHQSNVIETPDIV
                     VKKH"
     gene            1783..2799
                     /locus_tag="PKI35_11285"
     CDS             1783..2799
                     /locus_tag="PKI35_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNX45010.1"
                     /translation="MQTVNIFLKDNFRKFKEQVFSITSDDEFSRLAVELFNVQYRHNG
                     IYRQFIDHLGIATASINNYSQIPFLPISFFKNHRIVTGETAVSAYFSSSGTSTTGLSK
                     HYIVDGEVYEKSFLKGFQLFYGQPSGYCILALLPSYLEREGSSLVYMVKRLITESRHP
                     DSGFYLHNLDELAVKLTDNSKRKQKTLLIGVSFALLDFIENFSIDFPGLIIMETGGMK
                     GRRRELIRDELHEKLCKGFGVGTIHSEYGMTELFSQAYSSGNGIFSTPPWMKILIRDT
                     HDPLSLATDGKTGGINVIDLANFHSCSFIATQDLGRRHPDGTFEVLGRFDDSDLRGCN
                     LMIS"
     gene            2844..>4828
                     /locus_tag="PKI35_11290"
     CDS             2844..>4828
                     /locus_tag="PKI35_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX45011.1"
                     /translation="MKKMMILMAIPLMMSIGGCKNKGMQPATGEASSITKTTVDKVIK
                     DLTAKYGESVKARLEKGVTQAAALWRSSDGSDKDFEKFCADNFMADTTKRTVLFSKLQ
                     TAFEVLWGNYNKMGLDLRIPVDLDQGELTPIDELMASYSPSAHLTDDMFQNKVAFITI
                     LNFPCYTLKEKTDLAPQWNRTQWAYARMGDVFTSRVPAELSQKVTEALAKSDLYISSY
                     NIMMGNLVDKTGKTYFPADMKLITHWGLRDELKSHYGKPDGLAKQQMIYSVMKRIIDQ
                     SIPEMVINNKDYQWNPADNKVMKNGKEIQFTSEPDKRYEMLLNDFKAEKAVDPYYPVY
                     PTYSQRAFELGMEIPEADVEKLFTSLITAPEIKQVADLISKRLGRKLQPFDIWYDGFK
                     SRSSLNEDDLTAKTSKLYPNTAAFEKNTVNILKKLGWSAERAAYIAAKIQVDPARGSG
                     HAAQAAMKGDKARLRTRITEKGMNYKGYNIAVHEFGHNVEQTIDLYDIDNYMMSGVPN
                     TAFTEALAFIFQNRDLELLGINDNNPDKEHLASLDAIWMSYEIMGVSLVDMQVWNWMY
                     TNPNATPAQLKAAVITSAKEVWNKYYAPVFGMKDEPILAVYSHMIDNPLYLPNYPVGH
                     LINFQVDGFMKGKNFASEVDRMFKQGRLIPQVWMKGAV"
BASE COUNT         1378 a         1111 c         1026 g         1313 t
ORIGIN      
        1 gacgatacca gacatcatag gcatttttaa ttgatccgta ggaatgcttt tggagaattg
       61 ccctgaatac ctgccagtgg taagggttac acgattgttt gatcgcttct ttcaaatcga
      121 gaggcgacat atggtgatgc gtacatttta taggagaggt tgtcggcccc tgacaggaat
      181 aataagtttc gggtgtgagt gttccctcct gcaatccgat gaggtcgttc acaattttaa
      241 aagtagaacc cggcggatac tgtcccatga tagcgcggtt gagcaaaggt ttaaccgggt
      301 cttcatttaa ttttaaataa ttttcacctc ttgtccttcc aatcaacaga ttggggtcat
      361 acgtagggct ggaaagaaag gctaaaattt ctccggtagc aggatcaata gctacaatac
      421 ttccccgttt acctttcatc agcaactctc cataagtttg caaatcggca tccaacgaag
      481 tatataaatc ctggcccatc acggctgcgg tgtcgtattt accattttgg aagctgccca
      541 tctcacggtt aaacacatct accatgcgga ttttcattcc tttttgtcct cgcaattcct
      601 tttcatacga tttctctatt ccgcttatac ctatgtaatc tccggatttg taatagggat
      661 ctttgtcgag cactccctga gttgcttcac caatgtaacc aagagtatgc gcagctatag
      721 gcttgggata ttgtcgcatg gttcgcggct gaacgtagaa tccctgaaat ttataaagct
      781 tttcctccag ataaccatag gtttctttag agatttgttt ttcaaaaggc gatgctttat
      841 agggagagta tactcttgct ttttcaatac gctcgagaaa accgtcgtaa tcaatgccaa
      901 tcaaacggca aagctccatc gtatcaatat tttttacctg attgggcgtt accatcaggt
      961 cgtaaacggc ttcattataa acaagcaacc tgcccttacg gtcataaatc aatcctcgtc
     1021 ccggatattg ggttacataa cgaagtacat tgttatttgc cgataatgaa taacttttat
     1081 caattacctg gatataaaac aatcggaaaa taaaaataat acctgccaac agaaagacgc
     1141 caataataac gtactgtctg tttgaaaagt aggtattctt catagtgaat gggatgcttt
     1201 ggggtattaa tcaataaaat tacaacccgc aaaagtactc agtttaagga tttgtttaat
     1261 gtttttttac aacaatatcg ggtgtttcaa tcacattgct ttgatgaagc gcccggtcaa
     1321 caatatatac ttcaaatttt atggtatcaa aagttgaggc cggattgttg acataaagcg
     1381 tatcctgaat atctcccttg atggatttaa tgtttccgct gggcgtaagc acaggaatac
     1441 gggcgttgag cgtagaaggt aaatctactt ttttataaac gccgttttgt ttttcaaaat
     1501 aagtaatata cagattgaaa tcgtaaggtg gtaacgtatc ggcctgagtc aaaccaatat
     1561 ctccgtcgcc atcggtaaat gaaaactgta agattcctct ttcggtgatg ctgtcctcat
     1621 tcagcagcaa tgtaaatccc tggtaactga taaaggggat caccggataa gcttcaggtt
     1681 tggaacatga aaagaatgtc aaacttaaac cgccaatgat aaaaaatact atttttgaat
     1741 aaaatttcat gcctgttttt aacgggttaa aattactaaa aattgcaaac cgtcaatatt
     1801 ttcctgaagg ataacttcag aaaatttaaa gagcaagttt tcagcatcac ttctgatgat
     1861 gagtttagcc gtctggccgt cgaactattt aacgttcaat accggcataa tggtatttat
     1921 cgtcaattca tcgatcattt gggaatagct acggccagta taaataatta ttcgcaaatt
     1981 cctttcctgc ctatctcttt tttcaaaaac catcgtattg taaccggcga gacggctgtc
     2041 agcgcttatt tttcgagcag cggaacctcg acaaccggac tcagcaaaca ttatatcgtt
     2101 gatggcgaag tgtacgaaaa aagttttctg aaagggtttc aattgtttta cggacagcct
     2161 tccggctatt gcatcctcgc ccttcttccc tcttatctcg aacgggaagg ttcgtcgctg
     2221 gtatacatgg tgaagcggct gattaccgaa agccggcatc ccgacagcgg tttctatttg
     2281 cataatctgg atgaattggc tgtaaaacta acggataatt caaaacggaa acaaaaaacc
     2341 ttgttgatcg gcgtcagctt tgctttgctc gattttatcg aaaatttctc tattgatttc
     2401 cccggactca tcatcatgga aaccgggggc atgaaaggca gacgcaggga actgatacgt
     2461 gatgagcttc atgaaaagct gtgcaaaggt ttcggcgttg ggactatcca ttccgaatac
     2521 ggcatgaccg agcttttctc gcaggcttat tcttccggca acggcatttt ttcgacaccg
     2581 ccctggatga aaatcctgat ccgcgacacc cacgacccgc tttcgctggc aaccgacgga
     2641 aaaaccggag gcatcaacgt aattgacctc gccaactttc acagttgttc ctttattgct
     2701 acccaggatt tgggcaggcg tcatcccgac ggcacctttg aagtcctggg acgcttcgac
     2761 gacagcgacc tgagaggctg caacctgatg atttcgtaaa aatttgtatt tttgaaaaaa
     2821 tattcaccca aacaaacaac ccgatgaaaa aaatgatgat tctcatggca attccgctga
     2881 tgatgtccat tggcggatgc aaaaacaaag gaatgcaacc cgccaccggc gaggcctctt
     2941 ccattacgaa aactaccgtt gataaagtaa tcaaagacct tacggccaaa tatggggaat
     3001 ccgtaaaggc acgtctcgaa aagggcgtta cccaggctgc tgcactctgg cgttcttccg
     3061 atggcagcga taaggatttt gaaaaattct gtgccgacaa ttttatggcc gataccacta
     3121 aacgtacggt gcttttcagc aaacttcaga ctgcctttga agtcctctgg ggcaactaca
     3181 acaaaatggg attggacctt cgcattccgg ttgatctcga tcagggcgaa cttacaccca
     3241 tagacgagct gatggcttcc tattcgcctt cggctcatct taccgacgac atgtttcaaa
     3301 acaaggttgc ttttatcacc attctcaatt ttccctgcta taccttgaaa gagaaaaccg
     3361 acctggctcc tcaatggaac cgcacccaat gggcgtatgc ccgcatgggc gatgtgttta
     3421 cttcacgcgt ccccgcggaa ttgagccaga aagttaccga agctcttgcc aaaagtgatt
     3481 tatacatctc ctcgtataat atcatgatgg gtaacctggt ggacaaaacc ggaaagacat
     3541 atttccccgc cgatatgaaa cttattaccc actggggctt acgcgatgaa ttaaaatcgc
     3601 actacggcaa acccgacggg ttggctaagc aacaaatgat ttattccgtg atgaaacgca
     3661 tcatcgacca gagcatcccc gaaatggtga tcaataacaa ggattaccag tggaatccgg
     3721 ccgacaacaa agtgatgaag aacggcaagg aaattcaatt tacttccgaa cccgataaac
     3781 gctatgaaat gctgctgaat gatttcaaag ccgaaaaagc cgttgatccc tattatcctg
     3841 tttatcctac ttatagccag cgtgcctttg agctggggat ggagattccg gaagccgacg
     3901 tggaaaaact tttcaccagt ctgattaccg ctcccgaaat aaaacaggta gccgacctca
     3961 tcagcaaacg cctcggacgt aaacttcagc ctttcgacat ctggtacgat ggattcaagt
     4021 cacgcagttc gcttaatgaa gatgatctga cagccaaaac atccaaactg tatcccaata
     4081 ctgccgcttt tgaaaagaat acggtcaaca tcctgaaaaa gcttggttgg tcagccgaac
     4141 gtgcagccta tattgccgct aagattcagg ttgatcctgc ccgtggctca ggccatgccg
     4201 cccaggccgc catgaagggc gacaaagcac gcctgcgtac ccgcattacc gaaaaaggga
     4261 tgaactacaa aggatacaac attgccgttc atgaattcgg acataatgtg gaacagacca
     4321 ttgaccttta cgatatcgat aactatatga tgtcgggcgt ccccaatacg gcatttaccg
     4381 aagccctcgc tttcatcttc cagaaccgcg acctcgaact gcttggaata aacgacaata
     4441 atcccgacaa ggaacatttg gcgtccctcg atgccatctg gatgagttac gaaatcatgg
     4501 gcgtttcgct ggttgatatg caggtctgga actggatgta taccaacccc aatgccacgc
     4561 ctgcccagtt gaaagcagcc gttattactt ccgccaaaga agtctggaac aaatattatg
     4621 caccggtatt cgggatgaag gacgaaccta tccttgccgt ttattcgcac atgatagaca
     4681 atccgcttta ccttcccaat tacccggtag gccatttgat caatttccag gtggatggtt
     4741 ttatgaaagg aaagaatttc gcatcggaag tggatcgcat gttcaaacag ggacggctta
     4801 ttcctcaggt ttggatgaag ggcgccgt
//
LOCUS       DAOPYV010000119         4807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_22551_length_4807_cov_10.769571, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000119 DAOPYV010000000
VERSION     DAOPYV010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22551_length_4807_cov_10.769571"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..730)
                     /locus_tag="PKI35_11295"
     CDS             complement(113..730)
                     /locus_tag="PKI35_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HNX45012.1"
                     /translation="MKIAQKYSHLNGEEYIMVHHNKLYKEIIDTIESIDASKYMTKES
                     KEKTMTGKMLYSPIELNKVFNEKFSRLGWNESRYHYYITVDQKVLPELIMLPYDKQKE
                     FLISKGINNPISSFKQTDFVKDQIAVEVQFGKYAFVAFDLFVKHLLFYSGGIINLGIE
                     VLPTKKMQSQMSSGVAYYEGEVYNVLRHGRNNPPVPLLILGIEQD"
     gene            complement(745..1710)
                     /locus_tag="PKI35_11300"
     CDS             complement(745..1710)
                     /locus_tag="PKI35_11300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNX45013.1"
                     /translation="MKAKEPLFEYISPNISHNIVNGDSLTVLKKIENRKFDLIITSPP
                     YNVGKSYETKTSIEKYLETQEEIINELVRTLSDKGNLCWQVGNYVEKGEVFPLDIFYY
                     QIFKNHGLKLRNRIIWYFGHGLHASNRFSGRYETILWFSKTDDYIFNLDNVRVPAKYP
                     GKLHFKGEKKGLPSGNPLGKNPSDIWEIVVNGWETAMWNIPNVKSNHPEKTKHPCQFP
                     IELVERCVLALTNDNSWVLDPFAGVGSTVIAAIKNNRNAIGIEKEAEYCTIANQRIKD
                     FNEGNLKIRPINKPIYNPSGKEKVSQVPKEWLQLEINNLNGKYNK"
     gene            complement(1864..3054)
                     /gene="ygeW"
                     /locus_tag="PKI35_11305"
     CDS             complement(1864..3054)
                     /gene="ygeW"
                     /locus_tag="PKI35_11305"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF005538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HNX45014.1"
                     /translation="MDQTFRQYSEKLEQCSSSLFQKDFLLTWEKSTDELKTVLTVAEA
                     LKYLREHNISSRVFDSGLAISQFRDNSTRTRFSFASAANLLGLAVQDLDEGKSQIAHG
                     ETVRETSAMISFMSDIIGIRDDMYLGAGNAYMREVGAALDAAFAGGVLPQRPGIINLQ
                     CDVDHPTQSMADLLHLKTVFGSLENLRGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRL
                     GMQVDLAFPEGYGLIPKVMELAKKQAAESGGSFRVLNSMDEAFRDADIVYPKSWAPYH
                     VMGCRTELLRAGDQNGLKELEKECLANNTRFREWECTGKKMKLTREGKALYLHCLPAD
                     ISGISCREGEVSAAVFNLYRTPLYRQAGFKPYVIAAMMLANRFENPGNVLKQLSENNR
                     KRML"
     gene            complement(3198..4385)
                     /locus_tag="PKI35_11310"
     CDS             complement(3198..4385)
                     /locus_tag="PKI35_11310"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HNX45015.1"
                     /translation="MSDIFRKIDTLASEYAGYTAGNLSRLVKIRSVSLGEQDVAEELK
                     RQMREAGFDEVRTDGLGNVIGRIGNGKRVLAFDGHIDTVDVGNRANWSFDPFSGEIRD
                     GYVHGRGSVDQKGGPAAFITAGRILKELAFDRDLTLYFTGTVMEEDCDGLCWKYLVEE
                     EKIRPGFVVITEPTNLNIYRGQRGRMEMEVAFRGVSAHGSAPERGKNAIYMASRACLE
                     IEKLNEKLTTDPFLGKGSVTVTEMLSGSPSLCAVADYARIHLDRRLTRNETKESAAAE
                     IREIVKDMDASPGVLFYEETSYTGMRYGMEKYYPAWQMAEDHPLVVDACLAFRELFGR
                     EPLVDKWTFSTNGVTINGFYDIPCIGFGPGNEALAHAPDERVPVSDLVAASAFYAALV
                     FTV"
     gene            4628..>4807
                     /locus_tag="PKI35_11315"
     CDS             4628..>4807
                     /locus_tag="PKI35_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX45016.1"
                     /translation="MKHKILLFVFAALLVAFNTQAQDFWELIPFPDSLGIADIAVNSQ
                     GTIFVGTVTDNCNFNG"
BASE COUNT         1201 a         1165 c         1002 g         1439 t
ORIGIN      
        1 gcgaccttta tacgaagacc tgcaaaacca tgatggggct ggaaagttaa aagaaggatg
       61 catcgtaata aagcgaaact actgaatgct tgtaataggc gtttcgttat ttttaatctt
      121 gttcaatccc taagattaaa agaggaactg gaggattatt tcttccgtgc cttaaaacat
      181 tatatacttc tccttcataa tatgcaactc cactactcat ttgtgattgc attttctttg
      241 ttggcaaaac ttcgattcca aggtttataa ttcctccaga atagaaaagc aggtgtttaa
      301 caaacaagtc aaatgctaca aaagcatatt taccaaactg aacctcaact gcaatctggt
      361 cttttacaaa gtccgtttgc ttaaatgatg aaataggatt attaatgccc tttgaaatca
      421 gaaattcctt ctgtttgtca tatggtaaca ttattaattc agggaggact ttctggtcaa
      481 ccgtaatgta ataatgataa cggctttcat tccaaccaag gcggctgaac ttttcattaa
      541 agactttatt caattctatt gggctataaa gcattttccc agtcatcgtt ttctccttgc
      601 tttccttggt catatacttt gacgcatcaa tgctttcaat agtatcgatg atttctttgt
      661 atagtttatt atggtgaacc atgatatatt cttctccatt caaatgggaa tatttttgag
      721 ctattttcat ttagtaaaat atatttattt gttatacttt ccgtttaaat tatttatttc
      781 taattgcaac cattctttcg gaacctgaga aactttttct tttccagatg gattatagat
      841 aggtttatta attggtctga tttttaaatt tccctcatta aagtctttga ttctttggtt
      901 ggcaattgtg caatattcag cttccttttc aattccaatt gcatttcggt tatttttgat
      961 ggccgcgatc actgtcgaac ctacaccggc aaaaggatcg agtacccaac tattgtcatt
     1021 ggtcaaggca agaacacaac gctcaactag ttcaatcgga aattgacatg gatgtttagt
     1081 cttttcagga tgatttgatt taacattagg aatattccac atcgctgtct cccagccatt
     1141 gacaacaatc tcccaaatat cgcttggatt tttaccaaga ggattacccg atggcaatcc
     1201 ttttttttct cctttgaaat gtagtttccc aggatatttt gcaggtactc ttacattatc
     1261 aaggttaaaa atatagtcat ctgttttgct aaaccatagg atcgtttcat atcttccact
     1321 aaatctgttt gaggcgtgta atccgtgtcc gaagtaccat ataattctat ttcttagctt
     1381 tagaccgtga tttttaaaaa tttggtagta gaatatatcc aaaggaaaga cttcaccctt
     1441 ctcaacatag ttacctacct gccaacaaag atttcctttg tctgatagcg ttctcacaag
     1501 ttcatttatt atctcttctt gtgtctccaa atatttttca atactagtct ttgtttcata
     1561 tgatttcccc acattatacg gaggtgaagt aattattaaa tcaaattttc tattttcaat
     1621 tttttttaag actgtaagac tatctccgtt aacaatatta tgggatatat taggactaat
     1681 atattcaaaa agaggttctt tcgctttcat ctttttcgat ttttcgttct acaaatttaa
     1741 ttctttataa actagtttcc tgcattgttt agtacggagt tttcaacgat tatttcagaa
     1801 atgaacataa actagaatat aaatttgaaa cttagaaaat gatgacgcta aaactggcct
     1861 gctttacagc atccttttcc tgttgttttc cgacaactgt ttcaatacat ttccgggatt
     1921 ttcaaaccgg ttggccagca tcatggcggc aatgacgtaa ggcttgaaac cggcctgcct
     1981 gtataaagga gtcctgtata gattgaaaac agcggccgac acctcccctt ccctgcagct
     2041 tatcccggaa atatcggcag gcaggcagtg caggtaaagg gctttcccct ccctggtgag
     2101 tttcatcttc ttcccggtgc attcccattc cctgaagcgg gtgttgttgg caaggcattc
     2161 cttttcaagt tctttcagcc cgttctggtc gcctgcccgc aacaattccg tgcggcaccc
     2221 catcacgtgg taaggagccc agcttttcgg ataaacgata tcggcatccc ggaatgcttc
     2281 gtccatcgaa ttcagcaccc tgaaacttcc gccgctttcg gcagcctgct ttttagccag
     2341 ctccataact ttcgggatca gcccgtaccc ttcggggaaa gcaaggtcaa cctgcatgcc
     2401 caggcgggtc atcagcccga tgatgccctg cggaaccgac aacggcttgc catagctggg
     2461 agaataggcc caggtcatgg ctattttctt tcccctgagg ttttccagcg acccgaaaac
     2521 cgttttcagg tgcaacaggt cggccatact ttgggttgga tggtccacgt cgcattgcag
     2581 gttgatgatg ccgggccgtt gcggcagcac gccgccggca aaggcggcgt cgagggcggc
     2641 gccgacctcg cgcatgtaag cgttgccggc gcccaggtac atatcgtcgc ggatgccgat
     2701 aatgtcgctc atgaacgaga tcatggcgga agtctcgcgg acggtctcgc cgtgggcaat
     2761 ctgcgacttg ccttcatcca ggtcctggac ggcaagtcca agcaggttgg ccgccgaagc
     2821 aaacgaaaag cgggtacggg tgctgttgtc gcggaactgc gaaatggcca gcccggagtc
     2881 gaataccctg gacgagatat tatgctcccg aaggtatttc agcgcttcag caacggtaag
     2941 gaccgttttc agttcgtccg tcgatttctc ccaggtaagc aggaaatcct tctgaaacaa
     3001 ggaggaagaa cactgctcta atttttctga gtactgcctg aaagtctgat ccatggtgat
     3061 tttttttccg ttcagattaa tcaatcaata aggtattttg agccggagat gtatagatgg
     3121 aaccggggta tttgtatgca tccaatcctt tttcccggtg aagcgctgcg aactcagcaa
     3181 ctccctggtt atctacatta tacggtgaac accagggcgg cataaaacgc cgatgccgct
     3241 accaggtcgc ttaccggaac tctttcgtcg ggagcatgcg ccagcgcttc gttgccggga
     3301 ccgaacccga tgcagggtat gtcgtaaaat ccattgatgg tcaccccatt ggtcgaaaag
     3361 gtccatttgt ccaccaaggg ttcccggccg aacaattccc tgaaagccag gcaggcatcg
     3421 acgaccagcg gatggtcttc agccatctgc catgcaggat aatatttttc catcccgtac
     3481 ctcatgccgg tatacgacgt ttcttcgtaa aaaagcaccc cgggggaagc gtccatatct
     3541 ttcacaattt cccgtatttc ggccgcagcc gattctttcg tctcgttccg ggtcagccgg
     3601 cggtcgaggt gtatcctcgc ataatcggca accgcgcaca acgacgggct gcccgacaac
     3661 atttcggtaa cggttacgct tcctttgcca aggaaagggt cggtcgtcag tttttcgttc
     3721 agcttttcaa tttcgaggca ggcgcgggaa gccatataga tggcgttttt gcccctttcg
     3781 ggcgccgagc cgtgtgccga aacgcccctg aaagccactt ccatctccat cctcccgcgt
     3841 tgcccgcggt aaatgttcag gttggtaggt tcggtgatga ctacaaaacc gggcctgatc
     3901 ttttcttcct ccacgaggta tttccagcag agaccatcgc agtcttcttc catcacggtg
     3961 ccggtaaaat aaagggtaag gtcgcggtcg aaggccagtt ctttcagtat ccggcctgcc
     4021 gttatgaaag ccgccggacc gcctttctgg tcaacggaac cgcggccatg aacatagccg
     4081 tcgcggattt cgcccgagaa cggatcgaaa ctccagtttg cccggttgcc gacatccacg
     4141 gtatcgatat ggccgtcgaa ggccagcacc cgcttcccgt tcccgatgcg ccctatcaca
     4201 ttccccagcc cgtcggtacg gacttcgtcg aaacccgctt ccctcatctg gcgtttaagt
     4261 tcctccgcta catcctgctc acccaggctg accgacctga ttttcaccag cctcgaaagg
     4321 ttgccggccg tgtaaccggc atactccgat gccagggtat ctattttccg gaaaatatca
     4381 ctcatatgac cctctttagt gactcaaaat tagtgaattc acagcagata atttcaaccg
     4441 gggcatgtcc tgttcctaaa ttccctgctt acatttacca tgagtaatta aaaagatata
     4501 ttccaagaac ctttgctttt gataataagt tattcataaa ttttaaccag gtaaccatga
     4561 agcccatact attattatat tcgggtgctt tttatatcca ccagccatca aacaaacaac
     4621 ctgccttatg aaacataaaa tactactctt tgtatttgcc gctttgctgg ttgcctttaa
     4681 tactcaggcg caggattttt gggaattaat ccctttccct gattctcttg gtatagctga
     4741 tattgctgtg aactcccagg gaaccatttt tgtcggcact gtaactgata attgtaattt
     4801 taacgga
//
LOCUS       DAOPYV010000120         4652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_23626_length_4652_cov_14.961714, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000120 DAOPYV010000000
VERSION     DAOPYV010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23626_length_4652_cov_14.961714"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1381)
                     /gene="fabF"
                     /locus_tag="PKI35_11320"
     CDS             complement(128..1381)
                     /gene="fabF"
                     /locus_tag="PKI35_11320"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNX45017.1"
                     /translation="MKLRRVVVTGLGALTPIGNSVPEFWNGLINGVSGAGPITHFDAA
                     KFKTQFACEVKNFDPLNFFDRKEVRKYDLFTTYGLVSADEAITDASLLSEDLNRDRVG
                     VIWASGIGGMSTFYEEVASFITGDGTPRFTPFLIPKMIADIASGLISIKYGFHGPNFS
                     TVSACASSANALIDAFNYIRLNKADAFVTGGSEAPINPAGVGGFNSMHAMSTRNDDPL
                     TASRPFDKDRDGFVIGEGGAALIFEDLDHALARGAKIYCEVAGAGMSGDAYHMTAPHP
                     EGLGAINSMRMALEDAGMNGTEIGHINTHGTATPLGDIAEIKAVVRIFGEHAAKIAVN
                     GTKSMTGHLLGAAGAAEAIASILAVNNDLIPPTINHFTDDEELANTVDFVFNKPTQRT
                     VNAALSNTFGFGGHNATLIFKKFEE"
     gene            complement(1396..1632)
                     /locus_tag="PKI35_11325"
     CDS             complement(1396..1632)
                     /locus_tag="PKI35_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNX45018.1"
                     /translation="MSDIATRVKAIIVDKLGVDEKEVTPEASFTNDLGADSLDTVELI
                     MEFEKEFNIAIPDDQAEKIGTVGDAIAYIENNAK"
     gene            1882..2490
                     /gene="purN"
                     /locus_tag="PKI35_11330"
     CDS             1882..2490
                     /gene="purN"
                     /locus_tag="PKI35_11330"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNX45019.1"
                     /translation="MVINSNPGATAPKPNLAVFASGNGSNAQRLAEFFRQDGSAVISV
                     IYSNKPDAYVNKRASLLGIPSVVFNRKTFYETDDVLHDLQTRSVDWIILAGFLWLMPD
                     NLLKAFAGKIVNIHPALLPHYGGKGMYGMKVHEAVIASGDRESGISIHYVNQFYDEGR
                     IIFQARCPVDPADTPDMLAQKVHQLEYEHFPRVVKELVTGKG"
     gene            complement(2687..4360)
                     /locus_tag="PKI35_11335"
     CDS             complement(2687..4360)
                     /locus_tag="PKI35_11335"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNX45020.1"
                     /translation="MKKFIITVVCMFLLVLVNPVNACTNFLITKGASADGSCMISYSA
                     DSHTLYGELYHWPAATYPEGSLLDVYEWDTGKYLGKIKQARQTYNVIGNMNEYQVAIG
                     ETTFGGREECVDTSAVMDYGSLIYIALQRSKTAREAIAVMTGLVAEYGYCSEGESFSI
                     SDPNEVWIMEMVGKGKPVAVKDKKGRITWKHNKGAVWVAFRIPDGYVSGHANQARITT
                     FPLNDPENCLYAPDVIAFARQEGWFKGEDKDFSFSDTYNPVNFEGARFCEARVWSAFN
                     RITKGMDKYLDYAMGENLKNRMPLYVKPDHKLTLQEVMGLMRDYYQGTPMDMSKDPGA
                     GPYGCIVRWRPLTWEVDGKEYFNERATSTQQTGFVFVAQSRPYIPAPLGGIIWFGVDD
                     TYSTVFTPMYTCMTEVPETYAVGNGDMMTFSDNAAFWVFNQVSNFAYTRYADMIPEIQ
                     KKQKELEENYIAMVPAIDKLAAGKYSSDPASAIKFLTDFSVTQGNNTVYTWKKLYTYL
                     FTKYMDGNIKTPTGEQNPKVSQPGYGETFYRRLVKETGDKFEVIGSSKE"
BASE COUNT         1155 a         1076 c         1180 g         1241 t
ORIGIN      
        1 aaggccagtt tatacaaaaa aatatttccg gggtaaaacc cgaaaatatt ttttatggca
       61 tcaaataatg ctttatcctc tgaaagggcc gctcttaacg gcctgattac ctcgaaacgc
      121 gacaagtcta ctcctcgaat ttcttgaaaa tgagtgttgc gttatgtccg ccaaagccaa
      181 aagtattgct caacgctgca tttaccgtcc gttgggtagg cttgttgaaa acgaaatcaa
      241 ctgtattggc aagttcctca tcatcggtaa aatggttgat ggtgggcggg atcaaatcgt
      301 tatttacggc cagtatagag gcgatggctt cagcagcgcc ggcggctccc aggaggtggc
      361 cggtcattga ttttgtgccg ttcaccgcta tttttgcagc atgttccccg aatatccgca
      421 ctacggcttt tatttcggcg atatcaccca ggggggtagc ggttccgtgc gtattgatgt
      481 ggccgatttc ggtgccgttc attccggcat cttcaagggc catgcgcatg gaatttatgg
      541 cgccgaggcc ttcgggatgg ggcgcggtca tatggtaggc atcgcccgac attccggctc
      601 cggccacttc gcaatatatc ttcgccccac gggcaagagc atgatcaagg tcttcaaaaa
      661 tcagggctgc gccgccttcc ccgatcacga atccgtcacg gtccttgtca aaaggacgcg
      721 aggccgtaag aggatcatcg tttcgcgtcg acatggcgtg catggaatta aagcctccta
      781 ccccggcggg gttgatggga gcttctgatc caccggtaac aaaagcatcg gctttattga
      841 gccggatgta attgaaggcg tcgatgaggg cattggccga ggaggcacag gcagatacgg
      901 tagaaaaatt cggaccgtgg aaaccatatt ttatggagat aagccctgag gcaatatcgg
      961 caatcatttt tggaatcagg aaaggcgtaa agcggggagt gccgtcaccg gtgataaaac
     1021 tggcaacttc ttcatagaat gttgacatgc ctccgatgcc ggaagcccag ataacgccta
     1081 cgcggtcgcg gttaagatcc tcggaaagga gggatgcatc ggtaatggct tcatcggccg
     1141 atacaagtcc gtaagtagta aaaaggtcgt atttccggac ttctttccga tcgaagaaat
     1201 ttagcgggtc aaaatttttc acctcgcatg caaactgagt cttaaactta gctgcatcaa
     1261 aatgggtgat gggcccggca ccactgaccc cattgatcaa cccgttccag aattccggaa
     1321 cactattgcc aattggagtc agtgcaccaa ggccggtaac aactactcgc cttaacttca
     1381 tcaaacgtga tttaattatt tcgcgttgtt ttcgatgtag gcgattgcgt cgccaacagt
     1441 tccgatcttt tcagcctgat cgtcgggaat agcgatgttg aattcttttt cgaattccat
     1501 gatcagctca acggtgtcca gcgaatcggc accgaggtcg ttggtgaaac ttgcttccgg
     1561 ggtgacttct ttttcatcaa cacccagctt atcaacgatg atagctttta ctcttgttgc
     1621 aatgtccgac attgtttttt cattaattgg tttggggctg caaagaaatg ccatttcata
     1681 ttaaaaacca actttcgatg taaatattta catgaaaatt gaattaatag attgataacc
     1741 agccatcagc aggatcctaa agccggactg tcaaaagccg tttcaggcgg gcgagaatcg
     1801 aaaatcagga ataatgttca gcattttgac ctcaacagga tgtattttca ctttctttgc
     1861 cagacctaat aaaattagca gatggtgatc aacagcaacc cgggcgctac cgctccgaag
     1921 ccaaaccttg ccgttttcgc ttcaggcaac ggcagcaatg cacaacggct tgccgaattc
     1981 ttcaggcagg acggaagcgc cgtgatttcg gtcatttatt caaacaagcc ggatgcttat
     2041 gttaataaga gggccagtct cctgggaata ccttcggtgg tcttcaacag gaaaacattt
     2101 tatgaaacgg acgatgttct gcacgatttg caaacgcgtt cggttgactg gattatcctt
     2161 gccgggttcc tctggctgat gcctgataac ctgctgaaag cgtttgccgg gaaaatcgtc
     2221 aacattcacc cggcgctgct tcctcattat ggcggaaaag gaatgtacgg gatgaaggtg
     2281 catgaagccg tgattgcctc cggcgacagg gaaagcggca tcagcatcca ttacgtgaat
     2341 caattttacg atgaagggcg gattattttt caggccagat gcccggttga tccggccgat
     2401 actcccgata tgctggccca gaaagttcac cagctcgaat atgaacattt tccgcgggtg
     2461 gtaaaggaac tggtgacggg gaaaggataa aaaagcctga gttgtgtttg cggctcctgg
     2521 cgggtaatta aaacaggaaa gcccgccttc agccgaaagc gggctttcac atggtattga
     2581 tgtaagaacg ttttgggagg aaagagatta gcttttagcg gtttgctgtc ttttcgtcag
     2641 cctcttgccc ccgttcttac ttattggcgt aagcgccgtt ttcggattat tccttcgagg
     2701 aaccgatgac ttcaaattta tccccggttt ctttcaccag cctgcggtaa aaagtttccc
     2761 cataaccagg ctggcttact ttcggattct gttctcccgt aggcgttttg atattgccat
     2821 ccatatactt ggtaaaaagg tatgtataga gttttttcca ggtatagacg gtattgttgc
     2881 cctgcgtcac cgagaaatcg gtaaggaatt taatggccga tgccggatcg ctgctgtatt
     2941 ttccggcggc taacttatca atagccggga ccatggcgat atagttttcc tcaagttcct
     3001 tttgtttctt ttgaatttcg gggatcatat cggcataacg ggtataggca aaatttgaaa
     3061 cctggttaaa cacccagaaa gcggcattgt cgctgaaggt catcatatcg ccgttaccca
     3121 cggcataagt ctccgggact tcggtcatgc aggtgtacat gggtgtaaat acggttgagt
     3181 aagtgtcgtc aacgccgaac catataattc cgccaagggg ggccggtata taaggcctcg
     3241 attgcgccac aaagacaaaa cccgtttgct gggtgctggt agcccgttca ttgaaatatt
     3301 cctttccgtc aacctcccag gtaaggggac gccagcgaac gatgcagccg taaggtccgg
     3361 cgcccggatc cttgctcata tccatgggcg tgccctgata ataatcgcgc atcaggccca
     3421 ttacttcctg taaggtaagc ttgtgatcgg gtttcacata cagcggcatg cggtttttca
     3481 ggttttcacc catggcatag tcaagatact tgtccatacc ttttgtaatc ctgttgaaag
     3541 cgctccagac gcgggcttcg caaaaacggg cgccttcaaa attcacggga ttgtaagtgt
     3601 cgctgaagct gaaatctttg tcttcacctt tgaaccagcc ttcctggcgg gcaaaggcga
     3661 tgacgtcggg ggcgtaaaga cagttttcgg gatcgttgag cgggaaggtg gtgatacggg
     3721 cctggttggc atgtcccgat acataaccgt cgggaatgcg gaaggctacc caaacggcgc
     3781 ccttgttatg tttccaggtg atgcggcctt ttttgtcctt gacggccaca ggcttgcctt
     3841 ttcctaccat ttccatgatc catacttcat tcggatcgct gatgctgaag gattcccctt
     3901 cgctgcaata accgtattca gcaaccagac ctgtcatgac ggcaatcgct tcgcgggccg
     3961 tttttgagcg ttgaagggcg atatagatca ggctgccgta atccataacg gcagaggtgt
     4021 ctacacattc ttcccggccg ccgaaggtgg tttcgcctat cgctacctgg tattcgttca
     4081 tattcccgat tacattatag gtttgacggg cctgtttgat ttttccgagg tattttcccg
     4141 tatcccattc ataaacgtca agtaatgacc cttccggata ggtggcagca ggccagtggt
     4201 acaattctcc gtaaagggta tgcgaatcgg ccgaataact gatcatacag gatccgtcgg
     4261 cagaagcgcc cttggtaata aggaaattcg tgcaggcatt taccggattg accagaacga
     4321 gtaaaaacat gcatacgaca gtgatgatga attttttcat gagttggtat gggttgaagt
     4381 aaaaaactgg attgatttgt tcgcgacaaa atcagactgc acaaaattaa tgttttaggc
     4441 aatacggtac gtcggtgagc gtgtttgaaa atgagatcgg aatatatgga gcgtgaatag
     4501 ggttggttgt agttaaaacc acgaattttc acgaaatgaa cacgaatggg cacgaattag
     4561 aattgtaaga tacaggcttt cgtgataatt cgtgtttcgg cgcagccgga ttcgtgtaat
     4621 tcgtggttgc ttttttcacc acgaatgaac ac
//
LOCUS       DAOPYV010000121         4105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_28456_length_4105_cov_13.818765, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000121 DAOPYV010000000
VERSION     DAOPYV010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28456_length_4105_cov_13.818765"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1256)
                     /gene="tgt"
                     /locus_tag="PKI35_11340"
     CDS             complement(126..1256)
                     /gene="tgt"
                     /locus_tag="PKI35_11340"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNX45021.1"
                     /translation="MHFEIKATDASTSARRGQITTGHGVIDTPAFMPVGTVGTVKAVH
                     FRELRDDIRAQIILGNTYHLFLRPGLSILEQAGGLHKFNGWNGPILTDSGGYQVYSLA
                     ERRKLQNEGVVFHSHIDGSRHTFTPEIVVDIQRSIGADIIMAFDECTPYPCDYAYAKR
                     SLSLTHQWLERCLIQFKNTEPRYGYSQSLFPIVQGSVYRDLRMQSAEVVARCDADGNA
                     IGGLSVGEPVDQMYEMTELVCGILPYARPRYLMGVGTPANILESIALGIDMFDCVMPT
                     RNGRNGMLFTAEGIINIKNEKWKADFSPIDVAGNAYVDHEYSKAYLRHLFTAGEMLGP
                     MIASIHNLAFYQWLVREARKHIEDGDFRAWKEQMVKRVTQRI"
     gene            1430..2566
                     /locus_tag="PKI35_11345"
     CDS             1430..2566
                     /locus_tag="PKI35_11345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX45022.1"
                     /translation="MIFEYYSQNPVFFWLLCLLAFTVVIQLVYYWGFFAKVAYFRHKN
                     DFTRKDEPVSVIICARDEYYNLKENLPLFLQQDYSNYEVVVVNHGSEDETQYLLEDFS
                     REYPHLKIVNLPDDLNFFTGKKFPLSIGIKSAQNDICLFTDADCAPRGTDWLRKMASN
                     FTFGTEIVLGYGAYQREKSLLNLLIRFDTLRIAMQYMGFALAGIPYMGVGRNLAYRKS
                     LFYRHGGFISHYRIRTGDDDLFINKAANRKNTRVEFRKESHTLSKPKHTLRQWIRQKR
                     RHLTSGGHYKQIHRFLLGLNGISQFLFYALIVFLLVEWYQPYIVLGLAALRLLTQLIV
                     IKRIMRRFAEKGFLLLVPFFELFLLILTPVLVMTNSFYKPPRWK"
     gene            2557..3159
                     /locus_tag="PKI35_11350"
     CDS             2557..3159
                     /locus_tag="PKI35_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX45023.1"
                     /translation="MEIKANQTDKALRDLKLVKRARETGDRKAYAELLQNNYESLYAL
                     LLRMTGDAGDADDLTMEAFSKAFNKLDQYTSDFAFSTWLFRIGTNNCIDFLRRTRKDF
                     NPDPDSFSDLPEEPAVNPTELPCLSPGPEEALISKQQAKQLRKIVESLKPHYRELVEM
                     FYFQEKSVEEIAGLLEIPESTVKVRLFRARELLYNILKRK"
     gene            complement(3215..>4105)
                     /locus_tag="PKI35_11355"
     CDS             complement(3215..>4105)
                     /locus_tag="PKI35_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNX45024.1"
                     /translation="ALVLSGGGARGIAHIGVIEELLLHGYTIRSVAGTSMGALIGGIY
                     AQGNLTEFREWLLTITRMEILKFIDLTTVKGGMVKGEKIVQAINRFIGDVNIEDLNIP
                     YTAIAVDLNRHAEVVFSSGRLMEAIRASISIPTVFLPVKKDDALLVDGGVMNPLPIDR
                     VLRHDGDILIAVNVNANLPYAVSPKNHEHAEYEKGYRGVIDNLNSRWSELFHYSKKSK
                     VNHEKPVGMFEVIAETLNLMQDRLMQDAVEKYKPDILIQVSNRMSTIFDFYKAELIME
                     EGRKACRKALSEVEVYDQGS"
BASE COUNT         1002 a         1061 c          925 g         1117 t
ORIGIN      
        1 gttctccgtt ttcctgtcct cctttctcct tgctccctgc tccatgctcc tcgctactta
       61 acctttgcct tttgaccttt tacctttgac ctttatctaa aaccgttccc tcttgctccc
      121 tcctactaaa tccgctgtgt caccctcttc accatctgtt ctttccatgc cctaaagtca
      181 ccatcttcga tgtgtttcct tgcttcacgc accagccact ggtagaaagc caggttatga
      241 atactcgcga tcatcgggcc cagcatctcg cctgcggtaa acaggtgccg aaggtacgct
      301 tttgaatatt catggtcaac ataagcgttt ccggcgacat cgatgggtga aaaatcggct
      361 ttccattttt cgtttttgat gttgatgatc ccttctgccg taaacagcat cccgttacgt
      421 ccgtttcggg tgggcatcac acaatcaaac atatcaatgc ccagggcaat actttcgagg
      481 atattggccg gtgttcccac ccccatcaga taacgcggcc ttgcatacgg aaggatcccg
      541 cagaccagtt ctgtcatctc atacatctga tcaacaggtt cgccaaccga caatccgcct
      601 atagcattcc cgtcggcgtc gcagcgggca accacctccg ccgattgcat tcttaaatcc
      661 cggtaaacgg aaccctgtac tatcggaaac agcgattgac tgtaaccata acgcggctcc
      721 gtgtttttga attgtatcag gcaacgttcc agccactgat gcgtgaggct caatgagcgt
      781 ttcgcatagg catagtcaca ggggtaggga gtgcattcat cgaacgccat gataatatcg
      841 gcgccgatgc tccgctggat atcgacgaca atttcaggcg tgaaggtatg gcggctgccg
      901 tcgatatgcg aatgaaacac cacgccttca ttttgcagct tgcggcgttc ggccagcgag
      961 tatacctggt aaccgccgct gtcggtgagt ataggcccgt tccagccgtt aaatttgtgc
     1021 aggccgcctg cttgttccag tatgctgagg ccgggccgta aaaacagatg ataggtattg
     1081 ccaaggatta tctgggcccg aatatcgtca cgcaactcac ggaaatgcac tgctttaacg
     1141 gtaccaactg tacctaccgg cataaaggcc ggcgtatcaa taacgccatg gccggtggtt
     1201 atctgccctc tccgggctga agtggatgca tcagtagcct tgatttcaaa atgcatgaat
     1261 gggtaacagg tttacttggg caaaattgaa caatacaacc cggcccgcct ttttaaaata
     1321 ttgtatttta agatcttatg cggcaaggaa gactgttata aactttttcc aaagataggg
     1381 cttaattctt atcagaataa atacttttgt cagttcaata atctccccta tgattttcga
     1441 atactattcg cagaatcctg ttttcttctg gttactttgc ttgttggcgt ttacagtagt
     1501 gatacagttg gtctattatt ggggattctt tgcaaaggtg gcatatttca ggcacaagaa
     1561 cgacttcacc cgtaaagatg aacccgtttc ggttattatc tgcgctcgcg acgagtatta
     1621 taatcttaaa gagaaccttc ccctgtttct tcagcaggat tattcgaact acgaggttgt
     1681 cgtggttaat catggctcgg aggacgagac acaatatctg cttgaagatt tttccaggga
     1741 gtaccctcac ctgaaaatcg taaatcttcc tgatgatctg aattttttca caggaaaaaa
     1801 attcccgctt tcaataggca tcaagtcggc ccaaaacgat atttgtcttt ttaccgacgc
     1861 cgactgtgcg cccagaggaa ccgactggct gcgaaaaatg gcctccaatt ttactttcgg
     1921 aacggagatt gtcctgggct atggcgccta tcaacgcgag aaaagccttc taaacctcct
     1981 gatacgattc gatacattgc gcattgccat gcaatatatg ggttttgcat tggcaggaat
     2041 tccctatatg ggagtcggga ggaatcttgc ttaccgcaaa tcgctgtttt acagacatgg
     2101 cggattcatc tcacattacc gtatccggac cggcgacgat gatttattta tcaacaaagc
     2161 ggcaaacaga aaaaacaccc gggttgaatt tcgcaaggaa agccataccc tgtcgaagcc
     2221 aaaacacacg ttgagacaat ggataaggca gaaacggcgc catctgacat cgggcgggca
     2281 ttataaacag attcaccgct tcctgctcgg ccttaacggc atttcccaat ttttgttcta
     2341 tgccctcatt gtcttcctgc tggttgaatg gtatcaacct tacatagtcc tgggattggc
     2401 tgctttgaga ctgctaaccc agctcatcgt tataaaaagg attatgcggc gttttgccga
     2461 aaaaggtttc ctgttgcttg ttccgttttt cgaactgttt ttgttaattc tcacaccggt
     2521 gcttgtaatg accaattcat tctataagcc gccacgatgg aaataaaggc taatcagacc
     2581 gataaagccc tccgcgacct gaaactggtg aaacgcgccc gcgaaacagg cgaccggaaa
     2641 gcttatgccg aattgctgca aaacaattat gaatcgttgt atgccttgct gcttcgcatg
     2701 accggcgatg ccggtgatgc cgacgacctc accatggagg ccttcagtaa agcattcaac
     2761 aaacttgatc aatacaccag cgattttgca ttcagcacct ggcttttcag gatcggcacc
     2821 aataattgta tcgatttttt gcggaggacg cgcaaggatt ttaatccgga tccggattca
     2881 ttttcggatc tgcctgaaga gcctgccgtc aatccgacgg aacttccctg tctttcgccc
     2941 gggcctgaag aggcgctgat ttcgaaacag caggccaaac agcttcgcaa aattgtggaa
     3001 tccctcaaac cgcattaccg cgaactcgtt gagatgtttt atttccagga aaaatcggtg
     3061 gaggaaatcg ccgggttact ggagattccc gaaagcacgg ttaaagtaag gctgttcagg
     3121 gcaagggaac tgctttataa tatcctgaag cggaaatagc cttccaaaag cattaaccgg
     3181 cattcccgat ctcccgggaa agtcaggccc tgtatcatga tccttgatcg tacacttcaa
     3241 cttccgaaag cgccttccgg catgctttcc ggccttcttc catgatcagt tcggccttgt
     3301 aaaagtcgaa tatggtcgac attctgttcg acacctggat taaaatatcc ggtttatatt
     3361 tttccacagc atcctgcatc agccggtcct gcatcaggtt cagggtttcg gcaatcacct
     3421 caaacatgcc gacaggcttt tcgtggttta ctttgctttt tttcgaataa tggaatagtt
     3481 ccgaccaacg gctgtttaaa ttgtcgataa ccccgcggta ccctttttcg tattccgcat
     3541 gttcatggtt tttgggtgag actgcatacg gcaggtttgc attcacgttc acggcgatca
     3601 gtatatcgcc gtcgtggcgc aggaccctgt cgataggcaa tgggttcatc actccgccgt
     3661 caaccaaaag cgcatcatcc tttttcaccg gtaaaaacac ggtagggatg gaaatggatg
     3721 cccggatagc ttccatcagg cgtccggaag agaaaaccac ttccgcatgc ctgttcaggt
     3781 caacggcgat agcggtataa gggatattca ggtcttcgat gtttacatcg ccgataaaac
     3841 ggtttattgc ctgtacaatc ttctcaccct tcaccattcc accttttaca gtcgtaaggt
     3901 cgataaattt caggatttcc atgcgggtaa tggtgagcag ccattccctg aattcggtaa
     3961 ggttcccttg ggcatatatg cctccgatca gggctcccat cgaagtgcct gccaccgagc
     4021 ggatcgtgta tccgtgcaac agcagttctt caattacccc gatatgggca attcccctgg
     4081 cgccgccgcc cgacagcacc agcgc
//
LOCUS       DAOPYV010000122         4085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_28666_length_4085_cov_11.737717, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000122 DAOPYV010000000
VERSION     DAOPYV010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28666_length_4085_cov_11.737717"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..1520)
                     /locus_tag="PKI35_11360"
     CDS             complement(225..1520)
                     /locus_tag="PKI35_11360"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNX45025.1"
                     /translation="MNYSEALHYLFEQLPMFHRVGAAAYKNNLDNTLAICRLLGNPEN
                     KFRSVHVAGTNGKGSVSHMLASIFQEAGYKTGLFTSPHLKDFRERIRINGKMIPRSEV
                     IRFIQEHRKDFEAIQPSFFEWTWGLASQYFAKQQVDIAILETGMGGRLDSTNVVIPEL
                     SVITNISFDHMQFLGDTLAKIAAEKAGIMKPGIPAVIGETHPETQPVFRQKALETDTT
                     VCFADETFAITGYCHTKHQTPMLRADVFNRETRNTRQFITPLAGIYQLKNIITVLQAV
                     DLLRDKGFNISEQNISGGIRNVLKNTHLQGRWQVIGHKPLTVADIGHNKDGITKVLEQ
                     VSLTRFQKLHIVTGVVSDKDVDAMLSLLPRQAAYYFCKADIPRGLDAGMLKEKAGSKG
                     LAGNTYTSVKKALEAARNAAGAEDLVLVTGSAFVVAEVV"
     gene            complement(1527..2666)
                     /locus_tag="PKI35_11365"
     CDS             complement(1527..2666)
                     /locus_tag="PKI35_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX45026.1"
                     /translation="MTTFYRYIKGLFIALPVLFAGISFAQKENESYIISSGCDSLKPG
                     ALSAGLVNNNFVKNNEYFGKFTKGITYIGSIIQPEITYAFSGKAGLTVGWYARYFYGL
                     EKINQSLPVIRFDYRFIKGGRMIFGQLQGQFNHRLIEPLYSSDNYFEKNPENGLQLII
                     DKSGINADIWLDWEHFLLPGDSNQEQITAGLNLSYKLINKEPVSVKACLQSVIHHHGG
                     QVDVMDTPLQTRLNIAPGLIVGYSPDSRFVTGASLSAWLVQALDQSPTPTLRYLRGYG
                     IYTAATAENPWATVMAAWWHGKDYFSPLGDYLFQSVSELDPFYHRNTRDLLNMKLLLN
                     REIARGVKAGFRFETYHDLRKNQIDFCYGLNILAQAGWGCKSKKK"
     gene            complement(2798..3616)
                     /locus_tag="PKI35_11370"
     CDS             complement(2798..3616)
                     /locus_tag="PKI35_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX45027.1"
                     /translation="MIPSEPYTENRNESKGRMIALIGTIVFHAVVLLMLVFFALRTPL
                     PLPGEEGLEVNLGNSDQGMGDIQPEEAAMAANASPAQNMPNSDEEAVTENTDETPAIE
                     KISKKTKQTAVTINKPVTKPTLEAAKEPVVNSKALYPGKGSKTAKGGNQGITGQPGDQ
                     GKPNGSPGATNYDGLGGKGSSGIGYDLGGRSAKNLPKPKYTSDEQGDIVVEIKVDRNG
                     QVIYARAGARGTTIPDQSMQRQAEAAARSSIFARDPSAPEEQRGTITYKFRKMK"
     gene            complement(3630..>4085)
                     /locus_tag="PKI35_11375"
     CDS             complement(3630..>4085)
                     /locus_tag="PKI35_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX45028.1"
                     /translation="ASNPRSMAIKSHNKVSLEFSSSTMSDLVFLLLIFFMLTSTLVSP
                     NAIKLLLPSSTSKTMARQTATVYINEQNQYFVNETPSTIENLQMSIAGVLAGQSEATI
                     VLRSDRTVPIQYVVNVIDAVNQLNESMKTKYKVILATKPVDKQQITSVP"
BASE COUNT          974 a          963 c         1011 g         1137 t
ORIGIN      
        1 acgaagaata cgaaacgcag agagttgcgg attttctcag tgaatctcag tgccccttag
       61 tggttccttt ttcttctgct acagagaaag taaacatatt aactgaccgc taaaagccaa
      121 aggccaacag ccaacagccc ataaacaaaa agctaccgga aacagaaatc cggaatcaaa
      181 tcctcaaacc gcgatccgga gcctggaaac actgactcag gcttttatac tacttccgcc
      241 actacaaagg cgcttcctgt aaccaggacc aggtcttcag ccccggcggc gtttcgggct
      301 gcctcaagcg ctttttttac ggatgtataa gtatttccgg ccaatccttt tgagccggct
      361 ttttctttca gcattccggc atcaagtccg cgcggtatat cggctttgca gaaataataa
      421 gcagcctgtc ggggcaacag cgacaacatg gcatctacat ctttatcgct gaccacgcct
      481 gtcacaatat gcaatttttg gaaacgggta aggcttacct gttccaaaac cttggtaata
      541 ccgtctttat tgtgcccgat gtcggccacg gtgaggggtt tatggccgat cacctgccag
      601 cgcccctgca aatgggtatt cttcagcaca tttctgatgc ctccggaaat attttgttcg
      661 ctgatgttga atcctttatc acgtaaaagg tcaacggcct gcaataccgt aataatgttt
      721 ttcaattggt aaatgcctgc caaaggggtt ataaattgcc tggtattccg ggtttcccgg
      781 ttaaaaacat cagccctgag catcggggtc tgatgctttg tatggcaata accggttatg
      841 gcaaaggttt catctgcaaa acaaacagtg gtatcagttt ccagggcttt ctgcctgaaa
      901 accggttgcg tttcaggatg cgtttcgcca atgaccgccg gaattcccgg tttcattatt
      961 ccggcctttt cggctgcaat tttagccagc gtatctccaa ggaattgcat gtggtcgaag
     1021 ctgatattgg tgatcaccga aagctccgga atcaccacat tggttgaatc aaggcgcccg
     1081 cccataccgg tttccaggat ggcaatatca acctgttgct tagcaaaata ttgggatgcc
     1141 agtccccagg tccattcgaa aaacgatggc tgaatggctt caaaatcttt ccggtgttct
     1201 tgaatgaagc ggataacttc agacctggga atcattttcc cgttgatgcg gatgcgttcc
     1261 cggaagtctt tcagatgggg cgatgtaaac agcccggttt tataacctgc ttcctgaaag
     1321 atggaagcca gcatgtgcga tacgctccct ttaccgttgg tgcctgccac atgaaccgac
     1381 ctgaatttat tttcagggtt gcccagcaac ctgcaaatgg ccagcgtatt atcgagatta
     1441 ttcttataag cagccgctcc tacgcggtga aacatgggca attgttcaaa aaggtagtga
     1501 agggcttcgc tgtaattcat gaaggattat ttctttttgc ttttacatcc ccagccggct
     1561 tgggcaagaa tgttcaggcc ataacaaaag tctatctggt tttttcgaag gtcgtggtat
     1621 gtttcaaacc tgaaaccggc tttcacgccg cgggcgattt cgcggttaag caataatttc
     1681 atgttaagca ggtcgcgggt attacgatgg taaaacggat ccagttccga aaccgactgg
     1741 aacaggtaat cgcccagtgg tgaaaagtag tcttttccat gccaccaggc tgccataaca
     1801 gtggcccagg ggttttcagc agtcgcggca gtgtaaattc cgtaaccgcg gagatatcga
     1861 agggtgggag tgggcgactg gtcgagcgcc tgaacaagcc aggccgaaag agatgcaccg
     1921 gtgacgaacc tgctgtcagg agaataacca actatcagcc ccggcgcaat gtttagccgg
     1981 gtctgcagtg gtgtatccat gacatccacc tgtccgccgt gatgatgtat aacggattga
     2041 aggcaggctt ttaccgaaac cggctccttg tttatcagct tataggaaag gttcaaaccg
     2101 gcagtaattt gctcctggtt cgaatctccg ggaagcagga agtgttccca gtccagccag
     2161 atgtccgcgt taattccgga tttatcaatt attagctgaa gcccgttctc cggatttttt
     2221 tcgaaatagt tatccgaaga atagagcggt tcaatgagcc ggtggttgaa ctgcccctgc
     2281 agttgcccga atatcatcct gcctcctttt ataaaacggt agtcgaaacg gataaccggc
     2341 agcgactggt ttattttttc aaggccatag aaatacctgg cataccaccc aacggtgagg
     2401 ccggctttcc cggaaaaggc ataggtgatt tcaggctgga ttatggaccc gatataggtt
     2461 attccttttg tgaattttcc gaaatattcg ttgtttttca cgaaattgtt gttgaccagc
     2521 ccggcagata aggctcctgg tttcagggaa tcacaccctg atgagatgat atagctttca
     2581 ttctctttct gggcaaagga gatgccggca aaaagaaccg gtaatgcaat gaaaaggcct
     2641 ttgatatatc ggtaaaatgt agtcatggta taaacaatat ggttgccgga atattgcgca
     2701 aaggtaccat gaaagcaggt acaggcatat atgattcttg cggggatttt taggcgaatg
     2761 aaagatattt aagggagagg actgatccgg tggcgcctta cttcatctta cggaacttat
     2821 aagtaatggt tcccctttgt tcttcgggtg ccgatggatc gcgggcaaat atggagctgc
     2881 gggcggctgc ttcggcctgg cgctgcatgg actgatcagg gattgtagtg cctcgtgcac
     2941 cggcgcgggc atagattacc tggccgttgc ggtcaacttt gatctcaacc acgatgtctc
     3001 cctgttcatc ggaagtgtat ttgggttttg gaagattttt tgcactgcgg ccgccaagat
     3061 catatccgat cccgctgctc ccttttcctc caagtccgtc gtagttggtg gctcccggag
     3121 agccgttggg tttcccctgg tcgccgggtt gtcctgtaat tccctgattg ccgcctttgg
     3181 cggtcttact gcctttgccc gggtaaagcg cttttgaatt gacaaccggc tctttggctg
     3241 cctcgagagt gggctttgtc acaggtttat ttatggtaac cgctgtttgt ttggtctttt
     3301 tactgatttt ctcaatagcg ggagtctcgt cggtattttc ggtaacagct tcttcgtcgg
     3361 aattcggcat attctgcgca ggggaagcat ttgccgccat agccgcttct tccggctgaa
     3421 tatcgcccat tccctgatca ctgttgccca aattcacttc caggccttct tctcccggta
     3481 aaggcagggg cgtacgcagg gcaaagaaaa cgagcattag taagacaacg gcatgaaaca
     3541 caatcgtgcc gataagcgcg atcatacggc ctttgctttc attgcggttt tcagtatagg
     3601 gctcagaggg tatcattcaa tcagaacctt taaggcacag aagttatttg ctgtttatca
     3661 acgggttttg tcgccagtat tactttgtat tttgtcttca tactctcgtt caactgattt
     3721 acggcgtcaa tcacattcac aacgtactga ataggcaccg tccggtcgct tcgcaatact
     3781 atggttgctt ctgactgtcc cgctaaaacg cctgctatcg acatttgcag gttctcgatt
     3841 gtggatggcg tttcatttac aaaatactga ttctgttcgt tgatataaac ggtggccgtt
     3901 tgcctggcca ttgttttact ggtgctcgaa ggcaataata actttatggc attagggctc
     3961 acgagcgtgc tggtaagcat gaagaagatc agcaacagga acaccaggtc cgacatggtg
     4021 gatgagctga attccaggct tactttattg tgcgatttta ttgccattga tcttgggttt
     4081 gatgc
//
LOCUS       DAOPYV010000123         3932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_30354_length_3932_cov_12.422234, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000123 DAOPYV010000000
VERSION     DAOPYV010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30354_length_3932_cov_12.422234"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..2236
                     /locus_tag="PKI35_11380"
     CDS             383..2236
                     /locus_tag="PKI35_11380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX45029.1"
                     /translation="MKNSLILSVIALCLCVSASPEQRAAVPQALKSKVTECRRVADEN
                     ANPLNFVPNRYTAAIGFPEEEIGYTVYDLQSNSCSPYGRLCRYDDGTFNAVWTRGTGP
                     TAYNDRGTGYNYFDGTSWGEIPAARLESQRTGWPSVAKLGPAGEAVISHRGAAGLMFL
                     KRAAKGTGTWTEATLPPPTGASGLFWARMITSGPDHNTVHLLAVTTPVANGGVKYEGL
                     DGALLYYRSTDGGETWDQNGVILPGLTSNKYYGFSGDFYTFAEPKGDNIAFVVTDPQN
                     DMFLMKSTDNGETWNKTIIWEHPYPHLANGVVTDTLWGCDGAASLAYDNSGKLYLAFA
                     VYRLIFTATGYTFWPGLSGITYWTEDMPVYTGGDQFHALHVDSLDAHGLLIGYYNIDW
                     NGNGTLDIIDAGGYNNGWASWPQLSFDDNNNAILIYSALMENYSNTAQNYRHILCRAS
                     SNGARDWGPVFDLTDDEIHMFDECCWPVISDESDELNWYFTYQLDTEPGTALGQDEDI
                     PGDNYFNFYLLSKIVNKIEEAPANLTDISSNYPNPFSDRTTVNVTLGKPTPVSVKVSN
                     VAGRQVEFHDYGILGAGVHTLTIGASKLAPGVYMYTVYAGQQTATKKMIVK"
     gene            2383..3042
                     /locus_tag="PKI35_11385"
     CDS             2383..3042
                     /locus_tag="PKI35_11385"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNX45030.1"
                     /translation="MLSSEIRHLILKDIKLELRQRYAFNGILLYVISTVFVCFLSFST
                     IIDLPAWNALFWIIMLFAAVNAVSKSFVQENISRQLYYYSLASPQGIILAKIIYNFLL
                     MTLLAALCLLAFIILMGSLITNYMLFITALLLGCFGFSSVLTLVAAIASRTNNNFALI
                     AILSFPIVLPLMLILMKVTLSALNQGSWVENSHYAIALILINAVVVVLSYILFPYLWK
                     D"
     gene            3073..3750
                     /gene="ccsA"
                     /locus_tag="PKI35_11390"
     CDS             3073..3750
                     /gene="ccsA"
                     /locus_tag="PKI35_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNX45031.1"
                     /translation="MNCDLKKNWWKLLGVLLVLYSVIAGFLVPAPELPVVHESVRNLF
                     FHVVMWFTMMVLFAISLAYSVRFLLRFDIRLDQVASQAVNTGLFFGIVGIITGMEWAA
                     FTWGTPWTNDPQLNGAAVTIMAYLAYGVLRRSVDEESKRARISAVYNIFAFVLLVVFI
                     GILPRMAAGSLHPATGGSPSTVVTMKAAMRWVFYPALTGWILVALWILQLRIRYTRLK
                     DLLYEEV"
BASE COUNT          970 a          895 c          960 g         1107 t
ORIGIN      
        1 tatatttcta cacagtgaac aatggcgcat caacttcgac ccacaaaatg atcgtgaaat
       61 aattccggtt gtttattaat acttaaagct ccctgagcaa tcagggggct ttttggtttt
      121 aatctccttc tgaggaagaa ataacgatgg ccttgatccg ggcagtaatt tccaaaatgg
      181 aaattttaac cggtcagttg taaagagaaa gggcattatg cctccaccgt cctgaaagta
      241 taaaggctat taatatgttg cttctgtgat tttttcgtgg gaaagttagt ttaatttcat
      301 atatttgtat tatactcata tgactcccgt attcctattc tgtttaatac gattttaatt
      361 aaccttatta atcaagtctg tcatgaaaaa ttctctgatc ttgtcagtga ttgccctgtg
      421 cttatgtgtt tctgcatctc cggagcaaag ggccgcagtg ccgcaggcat tgaaatcgaa
      481 agtaaccgaa tgcagaagag ttgccgacga aaacgctaat cctttaaatt ttgtacctaa
      541 caggtatacg gctgcaatcg gctttccgga agaagaaatc ggatataccg tgtacgacct
      601 gcaatccaat agctgctcgc cttacgggcg gctgtgccgg tatgatgacg gtacttttaa
      661 tgctgtctgg acccggggaa caggcccaac ggcatacaat gaccgcggta caggctataa
      721 ttatttcgac ggtacgtcat ggggtgagat acccgcagcc cggcttgaat ctcagcggac
      781 cgggtggccg tcggttgcca agctcggccc tgcgggcgaa gccgttattt cgcaccgggg
      841 cgcagccggg ctgatgttct taaagagggc tgcaaaagga acaggaacat ggacggaagc
      901 aaccctccct ccgccaaccg gagcttccgg tttattctgg gcccgcatga ttacaagcgg
      961 accggatcat aatactgtgc atcttcttgc cgtcacaacc cccgttgcca acggcggcgt
     1021 taaatatgaa ggactcgatg gcgccttact atattaccgg tcgacagacg gaggtgaaac
     1081 atgggatcag aacggggtca tacttcccgg tttaacgtcc aataaatatt atggatttag
     1141 tggcgacttc tatacatttg ccgaaccaaa gggcgacaat attgcttttg ttgtcaccga
     1201 tccccagaat gatatgttcc tgatgaaatc aaccgataac ggtgaaacct ggaacaagac
     1261 catcatatgg gagcacccct atcctcatct ggcaaatggc gttgtaaccg acaccctttg
     1321 gggctgcgac ggcgccgcca gtttagctta tgataattcc ggcaaacttt atcttgcttt
     1381 tgccgtttac cgtttgatat tcactgcaac gggctatact ttctggcctg gtttgtcggg
     1441 aattacctac tggaccgaag atatgccggt ttataccggc ggcgaccagt tccatgcgct
     1501 gcatgttgat tcccttgatg cccatggcct cctgatcggg tattacaata ttgactggaa
     1561 cggcaacggc acattggata taattgatgc cggagggtat aataacggat gggcaagctg
     1621 gccacagctt tctttcgatg ataataataa tgccatcctg atatattccg ccttgatgga
     1681 gaattatagt aatacagcac agaactacag gcatatattg tgccgtgcct caagcaacgg
     1741 ggccagggac tggggacctg tctttgacct gacggacgat gaaatccata tgtttgatga
     1801 atgttgctgg ccggtaatca gcgatgagtc tgacgaactg aactggtatt tcacctatca
     1861 actggatacg gaacccggaa cagctctggg gcaggatgaa gatatacccg gagataatta
     1921 tttcaacttt tacctgcttt cgaaaattgt caacaaaata gaggaggcgc ccgcaaatct
     1981 gaccgatatt tcttcaaatt atcctaaccc gttttccgac agaacaaccg tgaacgtaac
     2041 cctgggcaaa cctactcctg tttcggtgaa agtttcaaac gttgcaggca ggcaggtaga
     2101 atttcatgat tacgggatcc tgggagccgg cgttcatacc cttacgatag gcgcttcaaa
     2161 gcttgctccg ggagtctata tgtataccgt ttatgccggc cagcaaacgg ccactaaaaa
     2221 gatgatcgtg aaatgaaaac aggcgccttc tgacatgcat aggcttcttt attttcggga
     2281 agccttttta atttgttgta tgacgatata tttcttcccc cggttctgac aatttttcct
     2341 tcagcttttt gcgtaagttt gctatgtcac aaaaggttgg ccatgttatc gtcggaaatc
     2401 aggcatctta ttctcaagga tatcaaactc gaactgaggc agcggtatgc tttcaacggg
     2461 atattgctgt acgtgatatc caccgttttc gtgtgcttcc tttcattcag cacgatcatt
     2521 gatctgcctg cctggaatgc ccttttctgg atcataatgt tgtttgcagc agtaaatgcg
     2581 gtatcaaaga gctttgtgca ggagaatatc agcagacagt tatactatta ttcactggcg
     2641 agcccgcaag gcattatcct ggcaaagatt atctacaact tcctgctgat gactttgctt
     2701 gcggcgcttt gcttgctggc attcattatc ctgatgggca gtctgatcac caattacatg
     2761 ctttttatca ctgcgctgct gctgggttgt tttggattct catcggtgct cactttagtc
     2821 gcagcaatag cttcgcgcac caataataat tttgccctga tagccatcct gagttttccg
     2881 atcgtcctgc ctttgatgct gattttgatg aaagtcacgc tttcggcgtt gaatcaggga
     2941 tcctgggtcg aaaacagcca ctatgcaatt gccttgatac tgataaatgc ggtcgtggta
     3001 gtgctttcct acatcttatt cccatacctg tggaaggatt aataaaagtc tttcattaaa
     3061 attgatcctg gaatgaattg tgacctcaaa aaaaactggt ggaaattact cggcgtgctg
     3121 ctggtattat acagtgttat tgccggtttt ctggtcccgg cgccggaatt gccggttgtt
     3181 catgagtcgg tcagaaacct gttttttcat gtggtgatgt ggttcaccat gatggtgctt
     3241 tttgccatct cgctggcata cagtgtcagg tttctgctcc ggttcgatat ccgccttgac
     3301 caggttgcat ctcaggctgt caacaccggg ctgttttttg gtatagtcgg tataatcacg
     3361 ggtatggaat gggcggcctt tacctggggc accccctgga ccaacgatcc gcagcttaat
     3421 ggcgccgccg tgacgataat ggcttatctg gcttatggcg tgctccgccg gtctgttgat
     3481 gaagaaagca aacgggcaag aatttcggct gtttataata tttttgcctt tgtgttgctg
     3541 gtagtgttca tcggaatttt gccgcgtatg gcggcaggtt cgttgcaccc ggctaccggt
     3601 ggcagtccgt cgacggtggt aaccatgaag gccgccatga gatgggtctt ttatcccgcc
     3661 ctgacaggat ggatacttgt cgccttgtgg attctgcagc taaggatcag gtatacaagg
     3721 cttaaggacc ttctttatga agaagtataa ggctgccggc tatggacagg agaatttaat
     3781 acggaatgag caaggggcag ggagaacgaa aaacggagaa cggagaacgg gaatgggaag
     3841 ttcagaatgt tgaactttta acttttgaaa caatggaatc ctgtcccctc acccgagacc
     3901 tcgtggcagg caggcctgcc accttacaca ca
//
LOCUS       DAOPYV010000124         3765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_32343_length_3765_cov_11.885175, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000124 DAOPYV010000000
VERSION     DAOPYV010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32343_length_3765_cov_11.885175"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..176
                     /locus_tag="PKI35_11395"
     tRNA            89..176
                     /locus_tag="PKI35_11395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:123..125,aa:Ser,seq:gct)
     gene            231..590
                     /locus_tag="PKI35_11400"
     CDS             231..590
                     /locus_tag="PKI35_11400"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX45032.1"
                     /translation="MFDFEKLEVYQLVKEQTSLVFSQLNEISGIEDTVLSTWKNSGLQ
                     IMLHLVQASGRVGTSEKKTLLTSARGYVFECAAILDLVKTSGLIEPDKYEELYNNYER
                     ISKMLLGMYKSYENKAE"
     gene            complement(658..3180)
                     /locus_tag="PKI35_11405"
     CDS             complement(658..3180)
                     /locus_tag="PKI35_11405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX45033.1"
                     /translation="MKRISQYLTILLLLFSGFCNGLSGQEFHPGTLIPGKSNPDALYE
                     SRNGFRLPVSGTVRVLFVFAEYDYINGGDPTPGEGTAGWPSHSLPAWADSLADAYPSA
                     GNPTGVLTGYYRQASSGNYTILGDYLLAPDNGGVFRVQTDSAHAIAPDNASLINEVNK
                     KLQNSIITAHGLNGISYFDLWSIPDNEFGLPKKSPSDENPGKYDHIVFIWRNSAYDGI
                     GDYSFCSPGIMLGYGANTYSWFGTRDRQPTQIMIHEYAHLLYGGLDFHCGGGGWFTGG
                     DYWIPATGGWSNLGLSGSSLMTWNAWDRLRLDWKTPGNRFPVSARNESGTEEVNGELD
                     AANPAQAGIYALRDFVTTGDAVRIKLPYLDPDKEFPEYLWLENHNTESMNQCRWDKFL
                     WQDGNSCVQQAIYGLYAYMQADRETRSGRTFDDVFKGFAYFLRPVTAEGFYDKEFEAN
                     TVLNNCVNSSPMFPFTRLPENSNPLTGSGDQEFYAVDWNHDDILDHRDQYYTNIENIG
                     GEYYKCMYNNGHSRNVFTLIGNHKIGMGTNPSSSSMMTMVGYDTPVEGVKNVRKIYLN
                     GISVEMISQDSDGTIKVKVRFDDVSLNEDVRWCADEIVLNPIVSSPGFSLNLDNGRTL
                     ILDQGTTATRMTDPSAFNCRKIFTSPTEMVVNEDAVLHLSPDSRIILKNASSLTFRKG
                     SSCIVENGGLIELQSGTTLIFDEGSKLVIKGNGKLIARPLATLCISLGAECSFREGLH
                     NLKAGEGVIIPAGCYDLSGEAGIQVYPNPAEDFIEIDYMLSSGNIPAELAIYDARGRV
                     AARLTLDGEQGKKVFETNRFGSGIYFYRLTGNERISGKFIIR"
     gene            3401..3475
                     /locus_tag="PKI35_11410"
     tRNA            3401..3475
                     /locus_tag="PKI35_11410"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3436..3438,aa:Pro,seq:tgg)
BASE COUNT         1012 a          928 c          847 g          978 t
ORIGIN      
        1 cgtactctgc gttcttttaa ataaaatcta tacattaccg tggttttgaa aaattccagg
       61 gtaaagtaat ttagttcata accaaccagg agaggtgggt gagtggctga aaccaacagt
      121 ttgctaaact gtcgtactcg attagggtac cgggggttcg aatcccccct tctccgcaac
      181 gtaaaaacga gcaggtatcc tgctcatttt ttttaataaa gccggcactc atgtttgatt
      241 tcgagaaact ggaagtatat caacttgtta aagagcagac cagcctggtt ttctcgcaac
      301 tgaatgaaat aagcggcatt gaagataccg tgctcagcac gtggaaaaat tcgggcctgc
      361 aaatcatgct ccacctcgtt caggcatcgg gaagggttgg aaccagtgag aaaaagacat
      421 tgctgacatc agcccgggga tatgttttcg agtgcgctgc catccttgac cttgttaaaa
      481 catcaggact tattgagcct gataaatatg aagaactgta caataactac gaaaggattt
      541 cgaagatgct tttaggaatg tacaaaagct acgaaaacaa ggctgaataa tcacccccga
      601 aaagtttcct tcagttttaa gatcaacgcc gtaacgctgc cgcttcaggg aaaccggtca
      661 gcggatgatg aatttcccgg aaattctttc atttccggtt aaccggtaga aatagatacc
      721 gctgccaaaa cggtttgttt caaatacctt tttcccctgc tcaccatcca gggtaagccg
      781 ggcggcaact cttcccctcg catcataaat ggccagttcc gcagggatat tacccgaaga
      841 aagcatataa tcaatttcaa taaaatcttc agcagggttt ggataaacct gaatgcccgc
      901 ttctcctgac aaatcataac atcctgccgg gataattacc ccctcccctg ctttgagatt
      961 atgcagccct tcccggaacg aacattcagc acctaatgag atacagagcg tggctagcgg
     1021 acgggcaatc aattttccat tacctttgat taccagctta ctgccttcat caaagatcag
     1081 ggttgtgccg ctctgtaatt ctatcaaacc gccgttttca acgatgcagg atgatccttt
     1141 tctgaaggtc agggaggagg catttttcaa tattatcctg gagtccggac tgaggtgaag
     1201 tacggcatcc tcgttgacaa ccatttcggt gggagaagta aaaattttcc ggcaattgaa
     1261 ggctgacggg tccgtcatcc tggtagccgt ggttccctgg tcaaggatca gggtcctgcc
     1321 attgtcgagg ttcagcgaaa agcccgggga ggatacgatc gggttcagga ctatctcatc
     1381 ggcacaccag cgcacatctt cattaaggct gacatcatca aacctgacct tgacctttat
     1441 ggttccatcc gaatcctggc taatcatttc caccgaaatc ccgttgagat aaattttcct
     1501 gacatttttc actccctcaa cgggtgtatc atagccaacc atcgtcatca ttgacgatga
     1561 agaaggattt gttcccattc caatcttatg atttccgatc agcgtgaata catttctgga
     1621 atggccattg ttatacatgc atttgtaata ttccccgcct atgttttcaa tgttcgtata
     1681 atactgatcc cgatgatcca gtatatcatc atggttccag tccacggcgt aaaactcctg
     1741 gtctcctgag cctgtgagcg gatttgaatt ttcgggaaga cgcgtaaaag ggaacatcgg
     1801 ggaagagttg acacaattat tcagaacggt attcgcttca aattctttat cataaaagcc
     1861 ttcggccgtc acaggccgca ggaaatacgc gaaacctttg aatacatcgt caaaagtgcg
     1921 gcccgacctg gtttccctgt cggcctgcat gtatgcatac aacccgtata tggcctgttg
     1981 cacgcaactg ttcccgtcct gccacagaaa cttatcccac cggcattggt tcatcgactc
     2041 agtattatgg ttttcgagcc ataaatactc ggggaattcc ttatccggat caagatacgg
     2101 gagtttaatc cggacggcat ctcccgttgt tacaaaatcg cgcagggcat aaatgccggc
     2161 ctgggcagga ttggcagcat ccagttcgcc gttgacttct tctgtgccgg actcattacg
     2221 ggcagagacc gggaacctgt tccccggtgt tttccagtcg agccgcagcc tgtcccatgc
     2281 gttccaggtc atcagcgacg atcccgataa tccgagattg ctccatcccc ctgtagccgg
     2341 aatccagtaa tctccgccgg taaaccatcc gccaccgccg cagtgaaaat caagcccgcc
     2401 atataacagg tgggcatatt catggatcat gatctgtgtc ggctgcctgt cacgggttcc
     2461 gaaccagcta taggtatttg ctccatatcc cagcataata cccggagaac agaaggaata
     2521 gtcgccgatg ccgtcatagg cgctgtttcg ccatataaaa acaatatggt cgtatttccc
     2581 cggattttcg tccgacgggc tttttttagg caggccaaat tcattgtccg ggatggacca
     2641 aagatcaaaa tagctgattc cattcaaacc atgcgccgta atgattgagt tctgcaactt
     2701 tttattaacc tcatttatca ggcttgcgtt atcaggggcg atggcatgag cgctgtccgt
     2761 ttgaaccctg aagacgcccc cgttgtcagg ggcaagcaaa taatcgccca gtatggtata
     2821 attgcctgat gatgcctgac ggtaatatcc ggtcagcaca ccggttgggt ttccggccga
     2881 aggataggca tccgcaaggc tgtcggccca ggccggaagt gaatgtgaag gccagcctgc
     2941 agtaccttcg ccaggggtgg gatcgccgcc gttgatatag tcgtattcgg caaatacaaa
     3001 caaaaccctg acagtccccg ataccggcag cctgaagccg ttcctgcttt cgtagagtgc
     3061 atccggattt gatttccccg gaataagggt tccgggatgg aattcctgcc ctgataaccc
     3121 gttgcaaaag cctgaaaata aaagcaatag aatagttaaa tactgtgata tccgtttcat
     3181 ataaaatatt tcaccgtaac catatcaatt caaacgaaca acaggtgaat ttatgatatt
     3241 tcattattca gatttatgat accaatatgt attcctgctt catttcaggc ttacagaata
     3301 aaagtattat cccggcggta aacgaaaaac cttttttgtc gattgcggaa ataacactac
     3361 ttttgcacct ccttttccac agaggaataa ggcagattat cggggtgtag cgcagtccgg
     3421 ttagcgcacc tggtttggga ccagggggtc gatggttcga atccattcac cccgacaaag
     3481 caaaggcctc ttgcatcgca agaggttttt ctttttcgaa cgaagcgcaa acctgtttgc
     3541 aggctgaagt gagaaaaaga aaaacttccg caggaaggcc attgctttga atcccctctc
     3601 ccggatcaat gaaattaatc cattcacccc gacaaagcaa aggcctcttg catcgcaaga
     3661 ggtttttctt ttttgaacga agcgcaaact atcagaattc cggaggaaat aaaaaagcag
     3721 cataccctcc ggcatgctgc ataatcgctc ggataataat gaata
//
LOCUS       DAOPYV010000125         3638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_34053_length_3638_cov_11.358080, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000125 DAOPYV010000000
VERSION     DAOPYV010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34053_length_3638_cov_11.358080"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="PKI35_11415"
     CDS             complement(<1..1416)
                     /locus_tag="PKI35_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNX45034.1"
                     /translation="MKWNELLRRKSIEQLMEHAGDREGPGSMKRSLTRGDLTALGIAA
                     IVGAGIFSTIGNAAASGGPAVSLLFVFTAFACGLSAMCYAEFAAGIPVSGSAYTYSYA
                     SFGELIAWIIGWDLIMEYAIGNIAVAISWSGYFTGLMNGLGLHIPEFMSIDYLSAARG
                     YKESISLMQQGTQFIDLNSGLQEAFKAWTSAPELGSVRLIADLPAFGIVALISWICYK
                     GIRESKVAGDIMVLLKVGILLLVIGVGAFWVNPGNWNPFAPNGIAGVLKGVSGVFFAY
                     IGFDAISTTAEECKDPRRDLPVSMFLALLITTLLYVATSLVLTGMVPYSELGVSDPLA
                     YVFQKLGLTRLSGIIAVSAVIAMASVLLVFQLGQPRIWMSMSRDGLLPPVFSKLHPRY
                     KTPAFATIVAGVLVSIPSLFLNLTEVTDLTSIGTLFAFILVSGGILVINPRGGKRNGK
                     KGFSVPYANSRWFLLPVWLLVI"
     gene            complement(1474..3381)
                     /gene="dxs"
                     /locus_tag="PKI35_11420"
     CDS             complement(1474..3381)
                     /gene="dxs"
                     /locus_tag="PKI35_11420"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNX45035.1"
                     /translation="MSPVQANILSGINSPEDLRKLPVEQLPAVCNEIRQLILEVISRN
                     PGHLGASLGTVELATAIHYAFNTPEDKLVWDVGHQAYAHKILTGRKDVFYTNRTYKGI
                     SGFPRMSESEYDAFGTGHSSTSISAALGMAIASALSGDKKRQHIAVIGDGAMTGGMAM
                     EALNNAAVSKANLLVILNDNGIAIDKNEGGLSKALTNITTSATYNRFKDKIWHIMGGN
                     SRYGANSRAVVKQLSGAVKSTLLRGSNLFEAFGIRYFGPVDGHDVMRLVQLFRDLQHI
                     PGPKLLHVVTVKGKGFEKAEKEQTLYHAPGHFDKETGELKDVPCKNQPPKYQVVFGKT
                     IVELAEKNPKIVGVTPAMSTGCSLNMMMITMPNRAYDVGIAEQHAVTFSAGMAASGFV
                     PFCNIYSTFMQRAYDQVIHDVALQNLHVIFCLDRGGLVGEDGATHHGVFDLAFFRPIP
                     NLTIASPLNEAELRDMMFTAQLDGMGPWVIRYPRGRGVMPEWKTPFREMPVGKGQQLR
                     AGKSLAFLAIGHIGNTVMKACDILSENGVEAAVYNMRFLKPLDEALLHDVFKHFDKII
                     TIEDGTIIGGLGTAVMEFASVNGYHEARIHRLGIPDRFIEQGTPDELYHECGMDTEGI
                     VKAAEILKTLK"
BASE COUNT          884 a          956 c          961 g          837 t
ORIGIN      
        1 tatcaccaga agccagacag gcagcaggaa ccagcggctg ttggcgtaag gaacggaaaa
       61 accttttttc ccgtttctct ttcctcctcg cgggtttatt accagtattc ctcccgatac
      121 caggatgaaa gcgaacagcg tcccgatact cgtcaggtcg gtaacttcgg tgaggttgag
      181 aaaaagtgaa ggtatggaaa ccagcactcc tgcaacaatg gtagcaaagg ccggtgtctt
      241 ataacgcggg tgcagttttg aaaataccgg cggcagcaat ccgtcgcggc tcatgctcat
      301 ccagatgcgc ggctggccaa gctggaacac cagcagcacg ctggccatag cgatcacggc
      361 gctcactgcg atgatccccg aaagccttgt gagccccagc ttctgaaaaa cataggccag
      421 gggatcggaa acccccagct cggaataagg gaccattccc gtcagcacca ggctggtagc
      481 cacatagagc aaggttgtga tgagcagcgc cagaaacatg gacaccggca ggtcgcggcg
      541 gggatcttta cactcctcgg cggtggtgga gatggcatca aagccgatgt aggcaaaaaa
      601 caccccggac acgcctttca gcaccccggc gatgccgttc ggggcaaagg ggttccagtt
      661 gccgggattc acccaaaacg ccccgacgcc tatcacaagc aggagaatcc ctactttcag
      721 gagcaccatg atatcgccgg ctaccttgct ttcgcggatg cctttgtaac aaatccagga
      781 aatgagggcc acgatgccga aagcaggtaa atcggcgatc agccggacgc tccccaattc
      841 cggcgccgaa gtccatgctt tgaaggcttc ctgcaggccg ctgttcaggt cgatgaactg
      901 tgttccctgt tgcatcaggc ttatggattc cttatatccc ctggcagcgc tgagataatc
      961 gatggacatg aattcgggaa tatgcaggcc caggccattc atgagcccgg taaaatatcc
     1021 tgaccaggag atggcgacgg cgatgttgcc gatggcatat tccatgatga ggtcccagcc
     1081 gatgatccag gcgatgagtt ccccgaaact ggcgtatgaa taagtataag cgctcccgct
     1141 gaccggtatt ccggcggcga attcggcgta acacatggcc gaaagcccgc aggcgaaggc
     1201 ggtaaagaca aacaacaatg aaacggcagg cccgccgctg gcggcagcat tgccgatggt
     1261 gctgaaaatt ccggccccca cgatggcggc aatgccgaga gccgtaagat cgcctctcgt
     1321 caggctgcgc ttcatgctgc cgggaccttc cctatcgccg gcatgttcca tgagttgttc
     1381 aatgctcttc cggcgcagca gttcattcca tttcaccgca acttaatttt cggtaaatgt
     1441 ataaaaagaa aaagaaaaat gaaggatttt accctactta agagttttca ggatttctgc
     1501 tgcttttacg ataccttccg tatccatacc gcactcatgg tagagttcat cgggagtacc
     1561 ctgttcaatg aagcggtcgg gaataccgag acggtgaatc ctggcctcgt gatacccgtt
     1621 tacggaagcg aattccatca cggctgttcc caggccgccg atgatggtac cgtcctcaat
     1681 ggtgattatc ttgtcgaaat gtttaaaaac atcgtgcagc aaggcttcat caagtggttt
     1741 cagaaaccgc atgttgtaaa cggctgcttc gactccattt tcagaaagga tatcacatgc
     1801 cttcataact gtattgccga tatggcctat tgccaggaaa gccagggatt tccccgcgcg
     1861 taactgttgt cctttcccga cgggcatctc ccggaatggc gttttccatt cgggcatcac
     1921 gcccctgccg cggggataac ggatcaccca cggccccata ccgtcgagct gggccgtaaa
     1981 catcatatcg cgaagttcgg cctcgttcag gggagaggcg atggtaaggt tgggaatggg
     2041 acggaagaaa gccaggtcga aaacgccatg gtgcgtggcg ccgtcttctc ccacgagtcc
     2101 gccgcggtcg aggcagaaga tgacatgcag gttctgcagg gcaacgtcgt ggattacctg
     2161 gtcataggcg cgctgcatga aggtagagta tatattgcag aaaggcacga atccgctggc
     2221 agccatcccc gccgaaaaag tcaccgcatg ctgctcggca ataccaacgt catatgcgcg
     2281 gttaggcatg gtgatcatca tcatgttgag ggaacaccct gtcgacatcg ctggcgtcac
     2341 ccccacgatc ttcggatttt tctccgccag ttcaacaatc gtcttaccga ataccacctg
     2401 gtacttcgga ggctggttct tacagggtac atctttcagt tcaccggtct ctttgtcgaa
     2461 atgtcccgga gcatgataaa gggtttgctc tttttcggcc ttttcgaacc cttttccttt
     2521 caccgttaca acgtgcagaa gtttggggcc gggaatatgc tgaagatcgc ggaacaactg
     2581 gacaagcctc atcacatcat gcccatccac ggggccgaaa taccggatgc caaaggcctc
     2641 gaacaggtta ctgccacgta acagggtgga tttgacggcg ccgctcagtt gctttacgac
     2701 agccctcgaa ttggcgccat agcgcgagtt accgcccatg atgtgccaga ttttatcctt
     2761 gaacctgtta taggtcgccg aagtagtgat gtttgtcagc gctttcgaca atccgccttc
     2821 atttttgtcg atggcaatgc cgttgtcgtt caggataacc agcaggtttg ctttcgacac
     2881 agccgcattg tttaatgctt ccatagccat accgcccgtc atggcaccat cgccgatcac
     2941 ggcaatatgt tgccgctttt tgtcgcccga aagcgcagag gcgatggcca tccccagggc
     3001 cgctgaaatg ctggtagaag aatggccggt gccgaatgcg tcgtattcgc tttcgctcat
     3061 gcggggaaaa ccgctgatac ctttataggt cctgttggta taaaaaacat ctttgcggcc
     3121 ggtaaggatt ttgtgggcat aagcctgatg gccgacatcc cagacgagtt tatcttcggg
     3181 ggtgttgaag gcataatgga tggcagtagc aagttcaacc gtgcccaaac tggcacccag
     3241 atgaccggga ttgcgggaaa taacttcgag aataagctgc ctgatttcat tgcataccgc
     3301 gggaagctgt tctaccggca gtttccgtaa gtcttccggt gaattgattc ctgataatat
     3361 attggcctgt acaggagaca tgatagttgg ttgatgatgt aaaattactg ctttccgttg
     3421 aaaggaacct gaaagcggac aagtttatgg agattaaaat tggaggaagc ctcggggaac
     3481 cgttaattat gtaaatgtca ggagagaata acattatcaa ccacgaattt cacgaaacag
     3541 gcttcgcctg ttacacgaat taacacgaaa tgcctcccct gcaatctgaa aaaataattt
     3601 gtgtccattc gtgtttcatc cgtgctaatt cgtggtga
//
LOCUS       DAOPYV010000126         3624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_34241_length_3624_cov_12.665453, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000126 DAOPYV010000000
VERSION     DAOPYV010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34241_length_3624_cov_12.665453"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="PKI35_11425"
     CDS             complement(<1..1348)
                     /locus_tag="PKI35_11425"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNX45036.1"
                     /translation="MRTKLIPVIEVDQEKCVNCHACITVCPVKFCNDGSKNHVSVNSD
                     MCIACGSCIDACTHDARKYLDDTDVFFRDLEQGVPMVAVVAPSAAANFRDQYLRLNGF
                     LKKIGIKAVFDVSFGAELTIKSYLEYAGKHHPDTIISQPCPAIVTYIELYQPELIPYL
                     APADSPVMHTIKMVREFYKDYRDHKFVFIAPCAAKRREFEELMLGDYTITMKYLNEYL
                     TRNKITLADFPEVEFDNPPAERAVLFSTPGGLLRTAVRDNPDLINRTRKIEGPTIVYE
                     YLKKLPMMIEKGFAPFLIDCLNCEMGCNGGPGTLNRHESPDLIEHYVEKRNKEVQAKY
                     GTGSPVQRWQGKRRLHRVLDQYYDEKLYRRNYVNHHDNNTISHPGPQEFDTIYRKMNK
                     FSDTDHYNCSSCGYGSCEQMAVAIYNGLNKPENCHYFTRSLILNTAETITGTVTDLA"
     gene            1434..1904
                     /locus_tag="PKI35_11430"
     CDS             1434..1904
                     /locus_tag="PKI35_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNX45037.1"
                     /translation="MQNNEIFGFQIDHQRYAFPLYTVDRVLQATSLITIPNAPSLIHG
                     VIDYYGTLIPVVNLRKRLNLPDRSIGINDYFIVIDTPKRKLAVVIDDVDDVIIPAESN
                     IIPSSNLDPSLEPHSICRIDDGLILIYDMERLLSGEDDLMIQEALEKATTHPTI"
     gene            1901..3367
                     /locus_tag="PKI35_11435"
     CDS             1901..3367
                     /locus_tag="PKI35_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNX45038.1"
                     /translation="MISRQLLQEVSSQVANLLGWDLCHHRLSDLKRGLTATATELGVG
                     DTPEKIERWIQTTRWSQRELDILSSHLTVGETYFFREPASLKAFQLQVIPEIMNRRSG
                     SSQFIRLWSAGCCSGEEPYTLAILLNQEFLDLKDWNVHLLGTDVNQSFLEKARKGDYT
                     RWSFRETPPYIQQKYFSEKDGVWSIDDKIKRMVKFSALNLADDLYPSAATETLNMDVI
                     FCRNVLMYFTPVQIKQVVKRFYNSLNDSGWLVTSAVELNDQYFGEFETIKFDSCILYH
                     KSKVKPQLIVSRDVIRSTPRQVRSRSNTLKAVRPPVTSGTSANHRPNSEEKPKMAAEQ
                     AKELFGQGKYEQCIVLCESELNKSPDQPDLILFTVQSMANLGRLDQAHEWAEKLRTSP
                     NVKAEHLYLYAHILLEKNDFEEALKNLKRALYLNPHHLMCHFLLGNIYTRLDNKAAGM
                     KHYQNVRDLLASYQADDLVPDSEGLTAGRLETIVKSLN"
     gene            3375..>3624
                     /locus_tag="PKI35_11440"
     CDS             3375..>3624
                     /locus_tag="PKI35_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNX45039.1"
                     /translation="MARDPRELLQKRAHMIARRPAETEGASNTIMVVEFLLSPEWYAI
                     DTSFVTEVLPLGNLTSLPGTPPFVVGIMNLRGKIISILN"
BASE COUNT          982 a          838 c          849 g          955 t
ORIGIN      
        1 tggcaagatc ggtaacggta cctgttatcg tttcggccgt gttaaggatg agcgagcggg
       61 tgaaatagtg gcagttctca ggtttgttaa gtccgttata aatagctacc gccatttgtt
      121 cacaactgcc atagccgcac gaagagcagt tataatgatc ggtatcggag aatttgttca
      181 tttttcggta aattgtatca aattcctgag gtccagggtg actgatggtg ttgttgtcgt
      241 ggtgatttac gtagtttcgg cggtaaagtt tttcatcata gtattgatcc aatacccggt
      301 gcagccgccg tttcccctgc catctttgaa ccgggcttcc ggttccatac tttgcctgca
      361 cctccttatt gcgcttttca acataatgct cgatcaaatc gggcgattca tgccggttga
      421 gggtgccggg gcctccgtta cagcccatct cacaattcag gcagtcgatc agaaagggtg
      481 caaacccctt ttcaatcatc atcggaagtt tttttaaata ttcataaacg atggttggac
      541 cctctatttt ccgggtgcga ttgatcagat cgggattatc tctgaccgcc gtccgaagca
      601 atccgcccgg agtcgaaaac aacaccgctc tttcagcagg cggattatca aattcaactt
      661 cagggaaatc ggctaacgtg attttattcc tggtcagata ttcgttcaga tatttcatag
      721 ttatggtata gtcgcccagc atcagttctt cgaattcacg acgtttggca gcacaggggg
      781 caatgaaaac aaatttgtga tcgcgataat ccttgtaaaa ttcgcgaacc atcttgatgg
      841 tatgcatcac gggactgtcg gccggggcca ggtaggggat gagttcaggc tggtacaact
      901 caatgtaggt aacgatggcc ggacagggtt gagagatgat ggtatcggga tggtgtttcc
      961 cggcatattc gaggtatgat tttatggtca gctcggcgcc gaaacttaca tcaaacacgg
     1021 cctttatccc tattttttta aggaacccgt tcaatctgag gtactgatcc ctgaaattcg
     1081 cggcggccga aggagccacc acggcaacca taggcactcc ctgctccaga tcgcggaaaa
     1141 aaacatcggt gtcgtccagg tatttacggg catcatgggt acaggcatca atgcaacttc
     1201 cacaggcaat acacatatcg gaattcacgg agacatggtt cttgcttccg tcgttacaga
     1261 attttaccgg acataccgta atacaggcat gacagttgac acatttttcc tgatcaactt
     1321 caattaccgg aataagtttg gttcgcatag atttgaaata taggcaaagt taaaatcatc
     1381 agcattataa tgacaggttt tccttatctt taacgaaaat ttttcggtgg ctcatgcaaa
     1441 acaacgaaat attcggattt cagatagatc atcagcgata cgcattcccg ttgtataccg
     1501 ttgacagggt attgcaggct acttcgttga ttacgatccc caatgcccct tccctgattc
     1561 atggggtaat cgactattac gggactctta ttccggtcgt taacctcaga aaaagactca
     1621 atttacccga ccggtcgatc ggaatcaatg actattttat tgttattgat acacccaaac
     1681 gaaagctggc agttgtcatc gatgatgtgg atgatgtgat tataccggct gaatccaata
     1741 ttattccttc gtccaatctg gatccttccc tggaacctca ttcgatctgc cgcatcgacg
     1801 acggtctgat cctgatttac gatatggaac ggttgttatc gggtgaggat gacctaatga
     1861 tacaggaagc gcttgaaaaa gcaacaacac atccgacgat atgatttcaa ggcaactgtt
     1921 gcaggaagtc agttcccagg ttgccaatct gttaggttgg gatctttgcc atcatcgact
     1981 gtcggacctg aaacgcggac ttacggcaac agcaacagaa ctgggcgtcg gtgacacgcc
     2041 tgaaaagata gaacgatgga ttcagaccac ccggtggtcc caacgtgagt tggatatcct
     2101 ctcctcgcat cttaccgtag gcgaaaccta ctttttcagg gaaccagcca gcctgaaagc
     2161 ttttcagtta caggttattc ccgaaatcat gaaccggcgc agtggtagca gtcagtttat
     2221 acggctttgg agtgccggct gctgcagtgg ggaggaaccc tatacccttg ccattcttct
     2281 gaaccaggaa tttttggatt tgaaggattg gaatgttcac ctgctgggaa ccgatgtcaa
     2341 tcagtcgttt cttgaaaaag cgagaaaagg tgactatacc cgttggtcat tcagggagac
     2401 ccctccctat atccagcaga agtacttttc ggaaaaggat ggggtatgga gcatcgacga
     2461 caaaataaag cggatggtga agtttagcgc actcaacctg gccgatgacc tgtacccatc
     2521 ggctgcaacg gaaaccctga acatggatgt aattttctgc cgcaatgtcc tgatgtactt
     2581 cacacccgtc cagatcaaac aggtggttaa acgattctat aattccctga acgactcagg
     2641 gtggctggtt accagcgccg ttgaattaaa cgaccagtat tttggggaat ttgaaaccat
     2701 taaattcgat tcctgcattc tttaccataa aagtaaggtg aaaccacaac ttatcgtctc
     2761 aagagatgta atcaggagca cacccaggca ggtccgatcc aggtcaaata ctttgaaagc
     2821 ggtcagacct ccggttacat cagggacttc ggcaaaccat aggccaaatt cggaagagaa
     2881 gccgaaaatg gctgctgaac aggctaaaga actcttcggc cagggaaaat atgaacaatg
     2941 tatcgtactt tgcgaatcag aattgaacaa atcgcccgac caaccagacc tgatcctttt
     3001 tactgtacag tcgatggcta acctcggaag gcttgaccag gcccatgaat gggcagaaaa
     3061 actcagaacc agcccgaacg tcaaggcgga gcacctttac ctttacgctc atatcctttt
     3121 ggaaaaaaat gatttcgagg aggctctgaa aaacctcaaa agagcattat atttgaatcc
     3181 ccatcacctg atgtgccact ttctattagg gaatatttat acaaggctcg ataataaagc
     3241 agcgggaatg aaacactacc aaaatgtcag ggatttattg gcatcatacc aggctgacga
     3301 tctggtaccg gattcggaag gattgactgc cggcaggctg gaaaccatcg ttaaatcttt
     3361 aaattgatac ggtcatggcc agagatcccc gggaactttt acaaaaacgc gcccacatga
     3421 ttgcaaggcg accggccgaa accgaaggcg cctcaaatac tatcatggtg gttgaatttt
     3481 tgctttcccc ggaatggtat gccattgata cctcttttgt taccgaagta ctcccgctcg
     3541 ggaatctgac ttccctgccg ggaactcctc cttttgtggt aggaatcatg aatttaagag
     3601 ggaaaattat ttcgatcctg aatc
//
LOCUS       DAOPYV010000127         3349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_38413_length_3349_cov_9.551002, whole genome shotgun sequence.
ACCESSION   DAOPYV010000127 DAOPYV010000000
VERSION     DAOPYV010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38413_length_3349_cov_9.551002"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2537
                     /locus_tag="PKI35_11445"
     CDS             <1..2537
                     /locus_tag="PKI35_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNX45040.1"
                     /translation="LGAITDTALIAACGYQTGLQLKRLGVHIDFAPVVDINSNPANPI
                     INIRSYGEDAESVILRAGSFNRGLGKSVLTVAKHFPGHGDTDTDSHLALPVIQHSAET
                     LKAVDMKPFRTLQQDIDGIMVGHLYVPAIDSTSGLPASLSSVVIDSVLRKEMNFQGLI
                     FTDALDMKGVVDYAGPGDAEVKALLAGNDVLLLSQSVPMAVTAIRTAIDSGWLSSGLI
                     EMRCKRILRAKYDFGIRDRSATFVRTDNLFNDLNTAEANLLNRRLYENSVTLLKNERE
                     IIPVLKPNIFRIACLTIGNAVPGDFEDRLDMYAHADHFYCPREATQEEVSELLRQLEP
                     YQLLIIAVMNTHPSPARGFGISDITAALADTLMSIKPVILDLFSLPYSVGRFKKISDA
                     KAVLISYQDNPVIHDVSAQVIFGAIGAKGKIPVTASADYAVNTGLKSESGLRLKYSLP
                     ESAGVSSSKLAAIDSIVMDGIRNKAYPGCQVLVSKGNSVIYQKSFGFQDYSGIRKVVN
                     SDLYDLASITKVAATTISVMQLYDRRQFDINKPLSKYFPALSRTNKHGLQIKDILAHQ
                     ARLKEWIPFYKYTLKDGMPDPVYYSRTESDSFAVRVADSLFLSSDYPQVIIDTIIRSE
                     LLPKKEYKYSDLGFILIGKTIEKITRQSLDDYTTQNFYQPLCLDMTYKPLEKYSLDRI
                     APTEEDMVFRHQLIHGYVHDQAAAMLGGVAGHAGLFGNANDLAVLFRMLLQHGEYAGR
                     RYIDSATVELFTSQQFPGNRRGLGFDKPQLDRMAEGPACPAASEKSFGHTGFTGTYVW
                     ADPESDIIIVFLSNRVNPDAENQKLTRLNIRTRIQQAVYNAILSPD"
     gene            2746..3243
                     /gene="tpx"
                     /locus_tag="PKI35_11450"
     CDS             2746..3243
                     /gene="tpx"
                     /locus_tag="PKI35_11450"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNX45041.1"
                     /translation="MSVTKFKGNPVNTFGDLPVVGSKAPSFELVKSDLSDLKLEDLSG
                     KRVILNIFPSLDTAVCAASVRRFNVEAARLPNTVVVCISKDLPFAHSRFCTTEGIKNV
                     VTASEFRSNNFGKSYGVMMTDGPLRGLMARAVVVIDEGGKVVYHELVPEIAQEPDYES
                     AVAVR"
BASE COUNT          871 a          779 c          836 g          863 t
ORIGIN      
        1 cccttggcgc catcaccgat acggccctga ttgcagcgtg cggatatcag acggggctgc
       61 agcttaaaag acttggagta cacattgatt ttgcccctgt agttgacata aacagcaacc
      121 cggctaatcc gattatcaat atccgttctt atggtgaaga tgcggaaagc gtaatcctga
      181 gggctggaag ttttaacagg ggacttggca aatcggtttt aacggtggcg aagcattttc
      241 ccggccacgg cgataccgat accgattccc atctagccct cccggttatc cagcattcgg
      301 ctgaaacgct taaagccgtt gatatgaaac cattccggac ccttcagcag gatattgatg
      361 gcatcatggt cggccacttg tatgttccgg ccatcgattc tacatcggga ttgccggcat
      421 cactctcttc ggtggtcatt gattcggtac tgagaaagga gatgaatttt caggggctga
      481 tctttaccga tgcattggat atgaaaggcg tggtcgatta tgccggcccg ggagatgccg
      541 aggtgaaagc cctgcttgca ggaaacgatg tcctgctgct ttctcagtca gttcctatgg
      601 cggtcacggc aatcagaacg gctatcgaca gcggatggct ttcttccgga ctgatcgaaa
      661 tgagatgcaa gcggattctc agggctaagt acgattttgg cattcgagac cggtcagcaa
      721 cattcgtccg gacagataat ctgtttaacg atttaaacac ggccgaagcg aacctgctaa
      781 atcggcggtt gtatgaaaac agtgtaacgc tccttaaaaa tgaacgggaa ataatcccgg
      841 tgctgaaacc caacatattc aggatcgcat gcctgactat cggaaatgca gttcccggag
      901 atttcgaaga caggctcgac atgtatgccc atgccgatca tttttactgt ccgcgtgaag
      961 cgacacaaga agaggtttcg gaattattaa gacaactgga accttaccaa ctcctgatta
     1021 ttgctgtaat gaacacgcat ccttcgcccg cccggggctt tggcatcagc gacattactg
     1081 ctgcacttgc cgataccctg atgagcatta aaccggtaat acttgacctc tttagccttc
     1141 catacagtgt cggcaggttt aaaaagataa gcgatgcgaa agccgtctta atatcgtatc
     1201 aggacaatcc ggttatacac gatgtctcgg cccaggtgat ttttggcgct ataggggcaa
     1261 agggaaaaat accagttacc gcttcggctg attatgctgt taatacaggg ctgaaaagcg
     1321 aatcaggcct caggttgaaa tattcattgc ccgaaagcgc aggcgtatcg tcctcgaaac
     1381 tggcagcgat cgacagcatt gttatggatg gcatccgcaa caaagcttac ccgggatgcc
     1441 aggttctggt gagtaaagga aatagtgtga tctatcagaa atcattcggg tttcaggatt
     1501 attctgggat aaggaaagtc gtcaattccg acttgtacga cctggccagt attaccaaag
     1561 ttgccgccac caccatttcg gttatgcagc tttacgacag gagacagttt gatatcaaca
     1621 agccgctgtc aaaatatttt ccggcccttt cccgaaccaa taagcacgga ctccagatca
     1681 aagatatatt ggctcaccag gcgaggctta aagagtggat accgttctat aaatataccc
     1741 tgaaagacgg aatgccggat cctgtttatt atagcaggac ggagtcagac agctttgccg
     1801 taagagttgc cgatagcctt tttttaagca gtgattatcc ccaggtgatc attgatacta
     1861 tcatccggtc ggaattgttg cccaaaaaag aatataaata cagcgatctt ggttttatcc
     1921 tgatcggcaa aacaattgaa aaaattacaa ggcaatcgct cgacgattat acgacgcaga
     1981 atttttatca gcctttatgc ctggatatga cttacaagcc gttggaaaaa tatagccttg
     2041 accggattgc tccgaccgaa gaggatatgg tttttcgtca tcaactgatt catgggtatg
     2101 tacatgatca ggctgccgca atgctggggg gcgttgccgg gcatgcgggt ttgtttggca
     2161 atgccaacga tctggcagta ttgttcagga tgttgctgca acatggggaa tatgccgggc
     2221 gaagatacat cgacagcgct acggtcgagc ttttcacctc gcagcaattt cccgggaacc
     2281 gccggggact tggatttgat aagcctcaac tcgaccggat ggcggaaggg ccggcatgcc
     2341 cggcagcttc tgaaaagagt ttcgggcaca ccgggttcac gggcacctat gtgtgggccg
     2401 atccggaatc ggacattatt atcgtcttcc tgtcgaacag agttaaccct gatgcagaga
     2461 atcagaaact tacccggctt aatattcgta cgcggattca gcaggccgtg tataatgcca
     2521 tccttagccc tgattaagct ggcgttacgg cagtaatttg cagaagatta tcccgttttt
     2581 tgttgtatct tgcaacagaa acgaacaaaa ccatcgttga ttggtttact tagatcatcc
     2641 ggcgatgatc ctgccatgat tcttatcaga agcagtttag cctcatcgtc ataatttttc
     2701 agcctccaaa aacaaacatt attcctttca ccataacctt gtgatatgtc tgtaacaaaa
     2761 tttaaaggaa atccggtcaa taccttcggc gatttgccgg tagtgggaag caaagcccca
     2821 tcgtttgaac tggtcaaaag tgatctctcc gatcttaaac tggaagattt atcagggaaa
     2881 cgggtgatcc tgaatatttt ccccagtctc gacacggctg tctgtgctgc ttccgtcagg
     2941 cgtttcaatg ttgaagctgc ccggttgccc aatacggtgg tggtttgtat ttcaaaggac
     3001 ctcccttttg cccacagccg attctgcaca accgaaggaa ttaaaaatgt agtcaccgca
     3061 tcggaattcc gaagcaacaa cttcggtaaa tcatatggcg taatgatgac cgatggcccg
     3121 cttcgcgggt tgatggcccg ggccgtggtt gtaattgatg agggcgggaa ggttgtttat
     3181 catgaacttg tcccggaaat tgcgcaggaa cctgactatg aatcggctgt cgccgtcaga
     3241 tagaaaaact tgccgggttg aaaaagtgct gtatctttgc agcgctattg cccttttgcg
     3301 gatgtggcgt aattggtagc cgcgccagac ttaggatctg gtgccgaaa
//
LOCUS       DAOPYV010000128         3145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_42127_length_3145_cov_11.700000, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000128 DAOPYV010000000
VERSION     DAOPYV010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42127_length_3145_cov_11.700000"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKI35_11455"
     CDS             <1..473
                     /locus_tag="PKI35_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6377 domain-containing protein"
                     /protein_id="HNX45042.1"
                     /translation="LIKEEYIGHYMDQCSVYIDKLDNYRRLLHKTATAGKIDDLMHKI
                     KSTELIEEELSEFFHNFDMTFLQLFPNFVEDFNLLLSEGEAIQLKSGQLLNTELRIYA
                     LIRLGITDSVKISHFLRCSLSTIYNYRTKIRNKARDSRESFDNKVQLIGSSSGF"
     gene            808..>3145
                     /locus_tag="PKI35_11460"
     CDS             808..>3145
                     /locus_tag="PKI35_11460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX45043.1"
                     /translation="MKTYLPWILLLLCSMFLAVPEAGAQVKGKPVSGMVVDSKGEPLT
                     GVTVLLKGSANGTTTDVSGKFTLVTGNTANPVLVFSFVGYATQEVAATSTGEMRVVMA
                     ESDVQLNEVVVVGYGFQKKRDLTGSVSSVNTRELKALPVPSIGDALQGRSAGVQILSS
                     GTPGSNVTFRIRGTGTINNSDPLLVIDGVPTDIPLNAVSPDDIASIEILKDASAAAIY
                     GSRGANGVVLISTKKGTAEKNNLEFKMFTGVQTASNMVEMLNASEFAALHNEMMANNG
                     QALNPDFADPSSLGAGTDWLDALFRTAPIQNYSLSYSGGNAKSHYYVSGAVLDQQGIV
                     INTGYRRYTVQFNSDSKVFDWLKFSNNLTLNHDVKNSGDYNIRNAMSALPTQSIYNAD
                     GTYSGPIGKASWAGDIINPIGKAKLVDNKTDGYNVLGNISGEISILKDLKFKSTGGLE
                     AKFWDSRTWSPAYNWQPTPQANSYLYERYNKSLTYLWDNYFTYDTYFTPDHHLTVLAG
                     SSAQTNREDYIEGSVQGFSSDNTQQLTNGTTSATLDGDAYEWALMSFIGRANYDYKGK
                     YLVTATMRRDGSSRFGSNNRWGWFPSASVAWRMSQEDFLKDLTWLDDLKLRAGYGVTG
                     NQNIGNYSFASVLQTIQYVFNGQVVSAVVPQAIPNPSVRWEQVEQSNLGFDASMFNGR
                     LNVTLDAYIKNTKDMLVPMSVPISTGYSDIVVPSINAGEVRNKGIELNVTSTNTTGPV
                     VWTTSFNISANHNEVISLNDTVPIYTGSIGLNQNLSIQK"
BASE COUNT          860 a          768 c          756 g          761 t
ORIGIN      
        1 acctgatcaa agaggaatat atcggccatt acatggacca gtgttcggtg tacatcgata
       61 aactggataa ttacagaagg ctgctccaca aaacggcaac ggccggaaag atagatgacc
      121 tgatgcacaa gatcaaatca acggaactta tcgaagagga gctgtcggag ttctttcata
      181 atttcgacat gacctttctg cagctcttcc ccaatttcgt cgaagatttt aaccttcttc
      241 tttccgaggg cgaagccatt caactcaaat ccggtcagtt gctgaatacc gaactgagga
      301 tatatgccct gatcaggctg ggtataaccg acagcgttaa aatctcccat ttcctcaggt
      361 gctccttatc caccatttat aattacagga caaaaatccg caataaggcc agggatagca
      421 gggaaagctt tgataacaag gtacagctta tcgggagcag ttcaggtttt tgaaaagcct
      481 gccggccgga aacgccggca gcggtgaatt tccgtcccgg ttccgcggat tgacggatga
      541 tgaacatcca ttataaagtt ggattccgct ttggtcttgc cgggaaattc tgctacagcc
      601 gtactacttt ttttatgaat ccagaaaatg taaaaagtta taattcaaaa cgatagttaa
      661 catcctcccg ttttttgact acatcttttt aacccggcgg agcatcgggg aattgccttt
      721 ttatatttgc tggtacctgc gttgagacgc cggagcaata acagttaact atctatacta
      781 accatacgtt taacattaat gaaaaaaatg aagacttatc tgccatggat actgcttttg
      841 ctatgcagta tgtttctcgc tgttcccgaa gcgggagccc aggttaaggg caaaccggtt
      901 tcgggaatgg tcgtcgattc caaaggagag cctctgaccg gagttacggt tctgctaaaa
      961 ggttctgcta atggaacaac cactgatgta agtggaaaat ttaccctggt taccgggaac
     1021 acggccaacc ctgtactggt attttctttc gtcggatacg caacccagga ggttgcagca
     1081 acatcaaccg gggaaatgcg ggttgttatg gccgaatctg atgttcagct caacgaagtg
     1141 gtggttgtag gttatggttt ccagaaaaag cgggacctca ccggttcggt ttcctccgtc
     1201 aacaccagag agctgaaagc cttgcctgtg cccagtatag gcgatgcctt gcagggacgt
     1261 tcggccggtg ttcagatttt atcatcgggc actcccggca gcaatgtaac gtttcgcatc
     1321 cgggggacgg gtaccatcaa taacagcgat cctctgctgg ttatcgacgg ggtaccgacc
     1381 gatatacctt taaatgcagt aagcccggat gacatcgcca gcatcgaaat cctgaaagat
     1441 gcatcggcag cagccattta cgggtccagg ggcgccaacg gcgttgtgct gatttctacg
     1501 aaaaaaggta ccgccgaaaa gaacaacctc gaatttaaga tgtttaccgg ggtgcaaacg
     1561 gcctccaaca tggtggaaat gctcaatgct tccgaatttg cagcccttca taatgaaatg
     1621 atggccaaca acggccaggc gctgaacccg gattttgccg acccgtcaag cctgggagcc
     1681 ggcaccgact ggctggatgc tttattccgc acagcgccca ttcagaatta ctccctttca
     1741 tactcgggcg gtaatgcaaa atcgcattac tacgtttcgg gggcggtcct cgaccagcaa
     1801 ggtattgtca tcaatacagg ttacaggcgt tataccgttc aattcaacag cgattccaaa
     1861 gtctttgact ggctgaagtt cagcaataac cttaccctca atcacgatgt taaaaacagc
     1921 ggggactata acatcaggaa tgccatgtcc gccctgccaa cccaatccat ttacaatgcc
     1981 gacggtactt acagcgggcc tatagggaaa gcatcttggg caggcgatat catcaatccg
     2041 atcgggaaag ctaagttggt agacaataaa acagatggtt acaatgtttt aggcaacatc
     2101 tcgggtgaaa tttccatcct gaaagacctt aaatttaaat ccaccggcgg actggaagca
     2161 aaattctggg acagccgtac ctggtcgccg gcttacaact ggcagcctac acctcaggca
     2221 aacagttacc tttatgaacg atacaataaa agtcttacct atttgtggga taactacttt
     2281 acctatgata cctattttac acccgatcat catctcaccg tgctggccgg cagcagcgcc
     2341 cagaccaacc gcgaggatta tatagagggt tcggtccagg gattttcaag cgataatacc
     2401 cagcagttga ccaacggaac tacctctgcc accctggatg gagatgcata cgaatgggcg
     2461 ctgatgtcgt ttatcggcag ggccaattac gattataaag gcaaatatct ggttacggcc
     2521 accatgcgtc gtgacggaag cagccgtttc ggaagcaaca accgttgggg ctggttccca
     2581 tcggcttcgg tagcctggag gatgtcgcag gaagattttc tgaaagacct cacctggctc
     2641 gacgacctga aactccgtgc cggatacggg gttaccggta accagaacat cggcaattat
     2701 tcctttgcat cggtattaca aactatacag tatgtcttca atggccaggt ggtaagcgca
     2761 gtggtgccgc aggcaatccc aaatcccagt gtccgctggg aacaggtaga acaaagcaac
     2821 ctgggatttg acgcttccat gtttaacggc aggctgaatg taacccttga tgcctacatc
     2881 aagaatacca aggatatgct ggtacccatg agtgttccta tttcaacggg gtattccgac
     2941 atcgtagttc cgagcatcaa tgccggggag gtcaggaata aaggtattga gttgaatgtt
     3001 accagcacca atactaccgg tccggtggta tggacaacca gtttcaacat ttcggcaaac
     3061 cacaacgagg tgatcagcct caacgatacc gtacctattt atacaggcag catcgggctg
     3121 aaccagaacc tttcgataca aaaga
//
LOCUS       DAOPYV010000129         3067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_43723_length_3067_cov_11.648738, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000129 DAOPYV010000000
VERSION     DAOPYV010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43723_length_3067_cov_11.648738"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1945)
                     /locus_tag="PKI35_11465"
     CDS             complement(167..1945)
                     /locus_tag="PKI35_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoaconitate hydratase family protein"
                     /protein_id="HNX45044.1"
                     /translation="MNGKTFAEKVLGAATGAIVFRRPDIILTHDNTASIANTFKKMGG
                     EKVKDPDQLLIVLDHNAPPTTAELANDYQKIRDIVKEQGIKKFHDVGNGICHQIMSEY
                     ARPGMIIVGSDSHTSTAGAFNAFAAGIDRTESAGLWKQGETWFRVPDSIKITLTGKLP
                     QGVYAKDLALWIIGMIGSSGADYMSIEYHGDGVKTLSVADRMTISNLASEMGAKNAVF
                     PADEVLEKHLGHKVDGIWADPDAKYIREINIDLGALFPVVAAPHHVDNVKALAEVKGL
                     PLNQAMIGTCTNGRIEDLREAAAILKDQKIPDGFQLLVIPASKDIYLQAMKEGLIEIF
                     MKAGASVLASSCGPCLGTGQGIPADGYNIISTANRNFKGRMGNKNSNIYLASPAVVAC
                     SALKGQIADPRESEKQEVFPYKIEQSKTVDIQQGDDRYANGVWNYTDVDNLNTDQMFA
                     GNLTYAIKSSEADKILPNLFKGFDDSFADRVKEGDIVIAGANFGCGSSREHPSVGLAY
                     AGVKAVICKSVNRIFYRSSVNQGLPIILLPEAVNAYKQGDKVELDFANGKVTIAGKVF
                     TFAPLPDKLVGIFNAKGLVNFVKQAK"
     gene            complement(2140..2745)
                     /locus_tag="PKI35_11470"
     CDS             complement(2140..2745)
                     /locus_tag="PKI35_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX45045.1"
                     /translation="MNKKTIFLLALILTSGVQAFSQDKKAENNTLPIRDFRNVYKFSI
                     SEFTRNTFEMGYERFVAPSTSLYLIVGLTSKDDDWESIFGVRTEMQLKIHAYTSSKGK
                     AVHRLYFAPYIMNNYFEIKTTDWDHGSNEPKISDSFDAVGTGVLFGWSFSFAKRMNLD
                     VYTGGGIRKAIGAEVDPDDGVWDRAYTGITPRFGIDVGFLF"
BASE COUNT          767 a          728 c          715 g          857 t
ORIGIN      
        1 cgttctccgt cctccgttct ccgttttacc tttgcctttt gccctttgtc ttttgacctt
       61 taaaactcat tcctatcacg taaaggattt tatcccgggc aattctaccc aaaaaaaatg
      121 ccggatgatg atttcacccg gcagtattat aatcaaagac aaacagttat ttagcctgtt
      181 ttacaaagtt cacaagacct ttggcgttga atatcccgac cagcttatcg ggcaacgggg
      241 caaaagtgaa aaccttaccg gcaatggtca ccttgccgtt ggcaaaatcg agttcaacct
      301 tgtcgccctg cttgtatgca ttaacggctt cgggaagcag gataataggt aatccctggt
      361 taacggaaga acgatagaat atccggttaa cgcttttaca gatcaccgct tttacaccgg
      421 cataagccag ccctacgctc gggtgttcac gcgatgagcc gcagccaaag ttggcgcctg
      481 ctattacgat atccccttct tttacgcggt cggcaaagct gtcgtcgaaa cctttaaaca
      541 ggttgggcag aattttatcg gcttccgaac ttttaatggc ataggtaagg ttaccggcaa
      601 acatctggtc ggtgttcagg ttgtcgacat cggtataatt ccataccccg tttgcatagc
      661 ggtcgtcacc ctgttggata tcgacggttt tactttgctc tattttatag ggaaatactt
      721 cctgtttttc tgattcgcgc ggatcggcga tctgcccttt cagcgccgaa caggctacta
      781 cggccggcga agccaggtaa atgttactgt ttttgttccc catgcggccc ttgaagttgc
      841 ggttggcggt ggagatgata ttgtaaccgt cggcagggat accctggccg gttcccaggc
      901 atggtccgca agaggaagcc agaacgctgg ccccggcttt cataaagatt tctatcagcc
      961 cttccttcat agcctggagg tagatatcct tcgaggcggg gataaccagc aactggaagc
     1021 catcgggaat tttctgatct ttcaggatgg ccgcggcttc gcgcaggtct tcgatcctgc
     1081 cgttggtgca ggtgccgatc atggcctggt tgagcggaag cccttttact tcagctaatg
     1141 ccttcacgtt atcgacatga tgaggagcag ccacaaccgg gaataaggct cccaggtcaa
     1201 tgtttatttc ccttatatat ttagcatcag gatcagccca aattccgtca accttgtggc
     1261 ccaggtgttt ttcgagcact tcgtcggcag ggaatacggc attcttggcg cccatttccg
     1321 aagcaaggtt ggaaatggtc atacggtcgg caacggagag cgttttcacg ccgtcgccgt
     1381 gatattcgat gctcatgtaa tcggcgccgc tggaacctat cattccgatg atccacaacg
     1441 ccaggtcttt ggcataaacg ccctgtggca gttttccggt aagggtgatt ttaatgctgt
     1501 cggggacgcg aaaccacgtt tcgccctgtt tccataagcc tgccgattcg gtacggtcga
     1561 tacctgcagc gaaggcatta aaggccccgg ctgtgctggt atggctgtcg ctcccaacga
     1621 tgatcatccc tggacgggca tactccgaca tgatctggtg acatatgccg ttcccgacat
     1681 cgtgaaactt tttgattccc tgctccttca caatgtcgcg tattttctga taatcattgg
     1741 ccagttcggc ggtggtggga ggtgcgttat ggtcgaggac aatcagcaac tgatcgggat
     1801 cttttacttt ttcgccgccc atttttttga aagtgttggc gatgctggcc gtattatcgt
     1861 gggtaaggat aatatcgggc cggcgaaaga caatagcgcc tgttgctgcg cccaggactt
     1921 tttcggcaaa tgtttttccg ttcatgactt atttattgta aatgaatgat taataaacat
     1981 ggtaaaacgg gggtaaaatt cggaaagatg tttcaatcca ggaaacaaac ctgaaagaat
     2041 attttccatt ataaataact gattatttgt aaaataacaa agcccgcctt taaatcggca
     2101 ggctctgcaa aagattttca cgcggttttt ttcgccggct cagaataaaa agccgacatc
     2161 tatcccgaaa cggggagtaa tgccggtata ggcacgatcc cataccccgt catccggatc
     2221 aacttcagcg ccaatggcct tacggatgcc tcctccggta tatacatcaa ggttcattct
     2281 tttagcaaag gaaaacgacc agccgaacag gacgcctgtg cccaccgcat caaaactgtc
     2341 ggaaatcttt ggttcattac tgccgtggtc ccaatcagtg gttttaatct caaaatagtt
     2401 attcatgata taaggcgcaa aatagaggcg gtggactgcc ttccctttcg agctcgtata
     2461 ggcatgaatt ttaagttgca tttcagtcct gacgccgaaa atgctttccc agtcatcatc
     2521 cttggaagta agcccgacga tcagataaag actcgtcgaa ggggccacaa acctttcata
     2581 tcccatctcg aaagtattcc gcgtaaattc gctgatgctg aatttgtata catttcggaa
     2641 atccctgatg ggtagagtgt tattttcggc ctttttgtcc tgtgaaaagg cctgaacacc
     2701 agatgtaagg attagtgcca gcaggaagat tgttttttta ttcataacgc aaaaagtatt
     2761 agtaaaggat tgttggatat tgattaatgt cggctgtacg caaggtcaat ggcacaggaa
     2821 aatggtcatt caggaactgc ctgaaattgg cagtatagct caattttgaa gaacgatcgc
     2881 gctttttaac ccatctaacg gtgcaaagaa cgaaatattt catcacataa caaccaatac
     2941 ttaaaatact tcatacctga cgggcaatag tttttatgat gaaataaatt tatttgtgtt
     3001 tctgaccaga atatttaatt ttgcactccc gtttaccaat agaatgagta cgcgactgat
     3061 ccgcctg
//
LOCUS       DAOPYV010000130         2963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_46058_length_2963_cov_13.026479, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000130 DAOPYV010000000
VERSION     DAOPYV010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46058_length_2963_cov_13.026479"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PKI35_11475"
     CDS             complement(<1..288)
                     /locus_tag="PKI35_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX45046.1"
                     /translation="MLNNNAKVALGYGLSQEPTAKSQKVKAKSQKEKVKGQKAKGKGK
                     GAGRTESGKWKAESGGRGAKNAKHKTQNAECRTQKGEGRTKNEKLCWQADLK"
     gene            456..1385
                     /locus_tag="PKI35_11480"
     CDS             456..1385
                     /locus_tag="PKI35_11480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNX45047.1"
                     /translation="MFRIRPLAGFKPFTQIIIFTGILGSCLLFSSLLGILLLMVIYGP
                     DSVAAVSNPSLYSTPSVIASLKIIQIVNQAFGLLFPAVLFALFASRNPGKYLRFGTRQ
                     WPVMMIASASLIVTAQPVIGWLGEMNARLVLPESLSGLETWLENSEQQAARLTEAFLS
                     TTTYTGLAVNVLMIGILPALAEEIVFRGVLQRLFGKLVGNMHWGIIISAIIFAGIHLQ
                     FYGFLPRFALGLVFGYLFLWTGNLWIPITAHFINNLLSVSTEFLFRKGLIGIDSTELG
                     NTGNIFLIVAAFIISGLLLFWFQRKRSGADLHD"
     gene            complement(1665..2135)
                     /locus_tag="PKI35_11485"
     CDS             complement(1665..2135)
                     /locus_tag="PKI35_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX45048.1"
                     /translation="MARFKRSGSRSTPGINTGSMSDIIFMFLFFFMVITTIREVTLEV
                     KVLPPQASEVQKLEKKSLVSFIYIGQPRKVSLGTESRIQLNNQFASVGDIQSFVAAER
                     ASRDESEKNFITTSLKVDMNTRMGIVTDVKQELRNAGAFKINYSTRKLAKKSED"
     gene            complement(2135..2707)
                     /locus_tag="PKI35_11490"
     CDS             complement(2135..2707)
                     /locus_tag="PKI35_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX45049.1"
                     /translation="MRKLPEINSGSMADISFLLLTFFLLTSSIDTDQGITRKLPPPPD
                     PNAKPPDIKERNVLKVLVNKSDRLLVQGQPIDIRQLKDRAKEFLTNPKNDPNLPEVEE
                     ITVNNLGTVRTTKGIISLKNDRGTSYDMYIQVQNELTAAINELRDDLSQQRFGTKFAD
                     LTNAAYSEAIQKAIPVAISEAEPENIGGQK"
     gene            complement(2727..>2963)
                     /locus_tag="PKI35_11495"
     CDS             complement(2727..>2963)
                     /locus_tag="PKI35_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX45050.1"
                     /translation="IKEGFGKSKGSLLYIGILAAILVIAYLISPAEQGAFYTKMGVGA
                     GTSKLIGAGLISTYIVGASLVVILLYSVVSKWFK"
BASE COUNT          686 a          728 c          622 g          927 t
ORIGIN      
        1 tttgagatct gcctgccagc ataatttttc gtttttcgtt ctcccttctc ctttctgcgt
       61 tctgcattct gcgttttgtg ttttgtgttt tgcgttcttt gctccccgcc ctccgctttc
      121 cgctttccat tttccgcttt ccgttctccc tgctcctttc ccttttccct ttgccttttg
      181 acctttgacc ttttccttct ggcttttagc cttcaccttc tggcttttag ctgttggctc
      241 ctggcttaac ccatatccta aggctacttt agcattatta ttcagcaagg agttttaagc
      301 ccagagggct ttaatactaa gaagcggtct cctctccttg cggatattta cgttcagact
      361 ccctgctccc ttgctccctg ctccctgcta aagattctgt ttattaaacc gtcccaaata
      421 cacgcttctg cgtatttttg ccgaaaacaa caccgatgtt tcgcatccgc ccccttgccg
      481 gtttcaaacc atttacccag atcataattt ttacaggcat attgggatcc tgcctgttgt
      541 tttcttcctt attgggcatt ttgctgctga tggtaatcta tggcccggat tctgttgccg
      601 ccgtttcaaa tccatccctg tactcaacac cttcagtgat tgcctcttta aaaataatcc
      661 agatcgtgaa ccaggctttc ggcctgcttt ttccagccgt tctttttgcc ttgttcgctt
      721 cccgcaatcc gggcaaatat ctgcgctttg gtacaaggca atggccagtc atgatgatcg
      781 cctccgcttc attaattgta acagctcaac ccgtaatcgg atggctgggt gagatgaacg
      841 ccaggctggt tcttcccgaa tccttatcag ggttagaaac atggcttgag aatagcgaac
      901 aacaagcggc ccggcttaca gaagcctttt tatctacaac gacttatacc ggccttgccg
      961 tcaatgttct aatgatcggc attctgccag cattggctga agaaatcgta tttcgcggcg
     1021 ttcttcaacg tttgttcggg aaacttgtcg gcaatatgca ctgggggatt atcatatcgg
     1081 caatcatatt tgccggtatc catttacagt tttacggttt cctgccgagg tttgccctgg
     1141 gccttgtctt cggttaccta ttcctgtgga ccggcaacct ttggattccg atcactgccc
     1201 attttatcaa taaccttctt tcggtatcaa ctgaatttct tttcaggaaa gggctgatcg
     1261 gaattgattc aacagaactc ggcaatactg gaaacatatt tcttatagtt gctgctttca
     1321 tcatttccgg cttgcttttg ttctggtttc agagaaagcg atcaggggcg gatctgcatg
     1381 actaaccact gccaggcatc cccggcaggt ttatcagaaa tgaacataag atttattaat
     1441 ttccctaccg gttctatccc cgggaagcca ggaattcgtt ttcctcttac cctgtttttc
     1501 caggcataga gacaaaaata ccccgggcgc catgcagctt tccgctttaa attattaaat
     1561 tcctggtact ttgcggcgga agacaataaa gcagtcaaag gattttgcag acaaaaaaaa
     1621 cgcccggtaa ataaccaggc gtttcactta aaagaattac acttttagtc ttccgacttt
     1681 tttgccaact ttcgggttga ataattgatt ttgaaagccc cggcattgcg aagttcctgt
     1741 tttacatcgg tgacaatacc catacgcgta ttcatatcaa ctttcaatga agtggtaatg
     1801 aagtttttct ccgattcatc acgagatgca cgctcggctg caacaaatga ctgaatatcg
     1861 cctactgagg caaactggtt gttcagttga atcctggatt ccgtaccgag agaaacttta
     1921 cgaggctggc cgatatagat aaagctcacc agcgacttct tttcaagttt ctgaacttct
     1981 gatgcctgcg gaggcaacac ctttacttca agcgtaactt cacggatggt agtaataacc
     2041 atgaagaaga aaaggaacat gaagatgata tcggacatgg aaccggtatt aataccgggt
     2101 gtggaacggc tgccgctcct tttaaaacgt gccattactt ttgtcctcct atgttttcag
     2161 gttccgcttc ggaaattgct acaggaatcg ctttttgaat ggcttcacta taggcggcat
     2221 tcgtaaggtc cgcgaatttt gttccgaacc gttgttgcga cagatcatcc cgaagttcgt
     2281 tgatagcggc tgtaagttcg ttttgaacct gtatgtacat gtcatacgaa gtaccacggt
     2341 cgttcttcag cgagatgatg cctttggtag tgcgcaccgt tccgaggttg ttaaccgtaa
     2401 tctcttctac ttcaggaaga ttgggatcgt tcttgggatt ggttaaaaat tccttggcac
     2461 gatcctttaa ctgacggata tcaatcggct ggccttgcac cagaagcctg tcactcttgt
     2521 tgacgagaac ttttaaaacg ttcctttcct tgatgtcagg cggctttgca ttgggatccg
     2581 gcggaggagg aagtttcctg gtaataccct ggtccgtgtc gatcgaagag gtcaacagga
     2641 agaaggtaag cagcaggaaa gagatatccg ccattgaccc actattgatt tcaggtagtt
     2701 ttctcatata aatcctattt acctgcttat ttaaaccatt ttgaaaccac ggaatacagg
     2761 agaattacaa cgaggcttgc accaacaata tacgttgaaa ttaatcctgc accgatgagt
     2821 tttgatgttc cggcaccaac gcccattttc gtatagaatg ctccctgttc ggcaggggaa
     2881 atgaggtagg ctatcacaag aatcgctgcc agaatcccga tatagagaag gcttcccttg
     2941 gatttcccga acccctcttt aat
//
LOCUS       DAOPYV010000131         2918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_47137_length_2918_cov_12.895913, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000131 DAOPYV010000000
VERSION     DAOPYV010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47137_length_2918_cov_12.895913"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2918
                     /gene="purL"
                     /locus_tag="PKI35_11500"
                     /gene_synonym="purI"
     CDS             <1..>2918
                     /gene="purL"
                     /locus_tag="PKI35_11500"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNX45051.1"
                     /translation="VISLKAETHNFPTTVEPFNGAATGSGGEIRDRMAGGKGSLPIAG
                     TAVYMTSYPRLQANNPWEQAMPPRKWLYQTPEEILIKASNGASDFGNKFGQPLICGSV
                     LTFEHSENNKNIAYDKVIMQAGGIGFARKQDSLKDNPEAGDKIIVLGGDNYRIGMGGG
                     AVSSVATGEYKNSIELNAVQRSNPEMQKRVFNAIRALAEMDVNPAVTIHDHGAGGHLN
                     CLSELVEATGGKIEINKLPVGDPTLSAKEIIGNESQERMGLILKEKDVDLLNKIAERE
                     RAPMYVVGETTGDHKFTFEDTTTGAKPMDLKMEDMFGKPPKTIIRDKTVNENFVPITY
                     DSKLLNKYLENVLQLEAVACKDWLTNKVDRSVSGRVALQQAAGKIQLPLNNLGAVALD
                     YQGVKGIATSLGHAPVAALVNPEAGSRLSLAEALTNIVFAPVEGGLEGVSLSANWMWP
                     CKNEGEDARLYKAVQALSEFAIALGINIPTGKDSLSMTQKYPDGETVLAPGTVIISAV
                     GEISNIRKIIKSVLQDVAGSGLIYINFSGNDFCLGGSSFAQTLNMVGNQVPDVKDALY
                     FKNCFNAIQQMIANDQIIAGHDISAGGLISTLLEMNFANTTTGMDTDLTALGNADIIQ
                     KLFSENPGVVLQVKNADEVCLFLKSHEISHAVIGKPSDKRNLTVQDHGTTYHFDIDHL
                     RDIWYHTSYLLDSKQCSGNHAKERFQNYKNQDLKFKFNDNFTGKASQFGIDLHRRQPT
                     GIRAAIIREKGVNGDREMAYSLYLAGFDVKDVHMTDLISGRETLEDISMIVFVGGFSN
                     SDVLGSAKGWAGAFLYNPTAKQALDNFYKRNDTLSLGVCNGCQLMVELDLINPDHDKK
                     THMLHNASHKFESAFLNVDVLENNAVMLRSFAGNRLGIWVAHGEGRFSFPYPEGNYHI
                     PVKYSYSEYPGNPNGSDFAAAAICSADGRHLAIMPHLERALFPWNWAEYPVDRKNDEV
                     S"
BASE COUNT          808 a          747 c          689 g          674 t
ORIGIN      
        1 gttatttcct taaaagccga aacccataac ttcccgacaa ccgtggaacc tttcaatggc
       61 gccgctaccg gctcgggagg cgaaattcgc gaccgcatgg ctggaggaaa aggtagtctt
      121 cccattgccg gaaccgccgt ttacatgaca tcgtatcccc ggcttcaagc caataatccc
      181 tgggagcagg caatgcctcc acgcaaatgg ctgtatcaaa cgccggaaga aattcttatt
      241 aaagcatcca acggtgccag cgatttcggt aataaatttg gtcagccgct catctgcgga
      301 agcgtgctta cctttgaaca ttcggaaaac aacaaaaata tagcttacga caaggtgatt
      361 atgcaggccg gaggcatagg ctttgcccgt aaacaggaca gtctgaagga caatcccgaa
      421 gccggcgata aaatcatcgt gttaggtggc gacaattacc gcatcggaat gggcggcggc
      481 gccgtttcgt cggttgctac cggcgaatac aaaaattcca tcgaactgaa tgccgtacaa
      541 cgttccaacc cggagatgca aaaacgcgtt ttcaatgcca tacgcgcctt ggcggaaatg
      601 gatgtaaatc ctgccgtaac catacacgac catggtgccg gcggtcatct gaactgcctt
      661 tcggaattag tggaagcaac cggcggaaaa attgaaatca ataaattacc cgtaggcgac
      721 cctaccctat cggccaagga aatcatcggc aacgaatcgc aggagcgcat gggtttaatc
      781 ctgaaagaaa aagatgttga tttactgaac aaaattgccg aacgtgaacg ggctcccatg
      841 tatgtggtag gcgaaactac cggcgaccat aagtttacct ttgaagacac aaccacaggc
      901 gctaaaccta tggacctgaa aatggaagat atgtttggca aacctcccaa gacaatcatc
      961 cgcgataaaa cggtgaacga gaatttcgtt cccataactt acgatagtaa acttctgaac
     1021 aaatacttag aaaatgtatt gcagttggaa gccgttgcct gcaaggactg gcttacgaac
     1081 aaagtagatc gctcggtaag cggacgggta gcccttcagc aggctgccgg taaaattcag
     1141 ctaccactga acaacctcgg cgctgtagct ctcgattatc agggtgtaaa aggcatagcc
     1201 acttccctgg gacatgctcc ggtggccgct ctggtaaacc ccgaagccgg ttcgcgcctt
     1261 tcgcttgccg aagcattgac aaacattgtt tttgctcctg tcgagggcgg cttagaggga
     1321 gtttcgctta gcgccaactg gatgtggccc tgcaaaaacg aaggcgaaga tgcccgtttg
     1381 tacaaagccg tgcaggcgct cagtgaattt gccatcgcct taggcattaa cattcccacc
     1441 ggcaaggatt ccctttcgat gactcagaaa tatcccgacg gcgaaaccgt actggctccc
     1501 ggaaccgtga ttatctccgc cgttggcgaa ataagcaaca tccgcaaaat catcaaatcc
     1561 gttttgcagg acgtcgccgg ctcaggtttg atttatatca atttttcagg aaatgatttc
     1621 tgccttggcg gaagcagttt tgcccaaaca ctaaacatgg tgggcaacca ggttcccgat
     1681 gtaaaagatg cgctgtattt taaaaattgt ttcaatgcca ttcagcaaat gatagctaac
     1741 gatcaaatca ttgccggaca cgatatttct gccggcggtt tgattagcac tctcctcgaa
     1801 atgaattttg ccaatacaac caccggaatg gataccgacc ttaccgctct tggcaatgcc
     1861 gacatcatcc aaaagctttt cagcgaaaat ccgggagtgg tgctccaggt aaaaaatgca
     1921 gacgaggttt gtcttttcct caaaagtcac gaaataagcc atgctgtcat cggtaaacct
     1981 tcggacaaaa gaaatttaac cgtacaggat cacggaacaa cttatcattt tgatattgac
     2041 catttgcgcg acatctggta tcatacctct tacctgctcg acagcaaaca atgctcagga
     2101 aatcatgcca aagaacgttt ccaaaactat aaaaaccagg atctgaagtt taaattcaac
     2161 gataacttca ccggaaaagc ctcacagttt ggtatcgacc tgcaccgtcg tcagcccaca
     2221 ggcatccggg ccgccatcat acgcgaaaaa ggcgtgaacg gcgaccgcga aatggcgtat
     2281 tcgctttacc ttgccggctt cgatgtgaaa gatgtgcaca tgaccgacct tatctccggc
     2341 agggaaaccc ttgaagacat cagcatgatc gtctttgttg gcggattctc caattccgac
     2401 gtgctgggtt cggccaaagg ctgggcggga gctttccttt acaaccctac ggcaaaacag
     2461 gcgctggata acttctataa acgcaacgat acgctgagcc tcggcgtatg caacggctgc
     2521 cagttgatgg tggaactgga tctgataaat cccgatcacg ataaaaaaac gcatatgctg
     2581 cacaatgctt cgcacaaatt tgaatcggct tttctgaatg tggatgtgct ggaaaataac
     2641 gctgtaatgt tgcgttcgtt tgccggaaac cgcctcggga tatgggttgc ccatggcgaa
     2701 ggacgttttt cgttccctta cccggaaggc aattatcaca ttcctgtaaa atacagctat
     2761 tccgaatacc cgggcaatcc caacggttcc gattttgccg ccgccgccat ctgttctgcc
     2821 gacggccggc atctggctat catgccccat ctcgaaagag ccttgttccc ttggaactgg
     2881 gccgaatatc ccgttgaccg gaaaaacgat gaagtttc
//
LOCUS       DAOPYV010000132         2656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_54489_length_2656_cov_10.762015, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000132 DAOPYV010000000
VERSION     DAOPYV010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54489_length_2656_cov_10.762015"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKI35_11505"
     CDS             complement(<1..500)
                     /locus_tag="PKI35_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX45052.1"
                     /translation="MKKLLLCFLATTIGMVAFAQFQRAALPKSVRDIAVSKVKAVTET
                     ENPLLKAGNTYVESPVSYMEDEVGYTVYDLQSNGSTPYGRLTQFTDGTFAAVYTVAVN
                     PTAYNDRGTGYNYFDGTTWGPSNSTRLESAKCGWPTHAQLGANGEVVVSHRSGTAGLK
                     LLKRDV"
     gene            complement(1347..>2656)
                     /locus_tag="PKI35_11510"
     CDS             complement(1347..>2656)
                     /locus_tag="PKI35_11510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX45053.1"
                     /translation="AAGLLWPRMVTGGTDKNTIHIIVLTSPTANGGTAYNGQDGALLY
                     MRSQDGGATWDKSEVLPGLGSELYSGFSGDNYSFAEPRGNTLAFVVTDDSQDLILMKS
                     TDNGESWTKTIIWARPYAAITAGADSMYSQDGGGHPVIDVNGKVHLFFGVYRTNPAEG
                     TWYPYIGSIAYWNENMPTWTGGTDEYQLNCLNPDTLDLTGQLVAYPLDLNGDGLWNVL
                     GEAGLYYCSPIGMPQALIDDDGDGFLIFSAITEGFDNDAQDYRHLWGRAIYNYGDDFG
                     CFFDLTGDDIHMFDECVFPSICATGDLLEGYKIMYQLDTEPGLSVRGDEDQPGDNYLN
                     IQHLVFPTGTEKPVQKALTVSQNLPNPFSGTTEVKVYLEKQAPVNIVVTNVSGQQVSN
                     INCGTLGTGIHTLTIDASNLSAGVYFYTVNNGASTSTHKMIVK"
BASE COUNT          725 a          606 c          625 g          700 t
ORIGIN      
        1 ttaacgtcgc gtttcagtaa cttgaggcct gccgttccgc tgcggtgcga aacaaccact
       61 tcaccgtttg cgccaagctg ggcatgagta ggccagccgc attttgccga ttcaagacgg
      121 gtggaatttg aaggtcccca ggttgtaccg tcaaaatagt tgtaaccggt accacggtca
      181 ttataagccg ttgggttaac agccactgta taaacagcag caaaggtgcc atctgtaaac
      241 tgggtaagac ggccataagg tgttgaacca ttcgactgca ggtcataaac agtataacct
      301 acttcgtctt ccatatagga aacaggcgat tcaacgtaag tgttgccagc ttttaaaaga
      361 ggattttcag tttcggtaac ggcctttact ttagaaacgg caatatcgcg tactgatttc
      421 ggaagcgcag cccgctggaa ctgggcaaag gcaaccatgc caatagtggt agccaagaag
      481 cagagtagta attttttcat gtttgaatta agtaaagtaa gtctttgatt tagatttcaa
      541 ctgtacagtg ccacaaaaat aataaaagtg tgtttctgat cacaataaga taaaaaattt
      601 aattactatt atttggtata ataaagagtt agtaagtatc ccgggaaata ttaactcttt
      661 ttactttttc cggtcaggta acaaaaaata tatagataaa aatccgcccg gaagcaacct
      721 ggttcgcagt acgctgtttt tgctggttcc tcttagtatt attattcagg cagttgcaga
      781 agcaatcagc ccgtaacctg aataattcta tcctgaaaat aagccgccgt aacagatttt
      841 tgttccccaa aaaaacacat tccccccttt taaaacatac ctgcctaagc ccaaaaaagc
      901 cgtgagtgag aataaaacgc atcatacagc cataatccct gccgggagaa gttcatacgg
      961 gaataccaaa ggatacggat aatccggctc tgcggattaa ttttcatttc cggtaaaact
     1021 gtacggccgg ttgccgatat tgctataatg aaaccggtcc ggtcatgacg ccctgccttg
     1081 taaaatattg ctgtggttga ttatttccat ctggttccct tcctccccgg tgaatcgttc
     1141 tacaatgttg agcccgactg atgatgatca attatcactt cgtgagaaca gtaatcccga
     1201 aaagtgcagg actgttacaa ccagacaccg gcccgttcca agacaaatta tcgggaatca
     1261 gcgccgaaaa catgaaaaat aattcataca aagtaaaagc tcccgttttt accggagctt
     1321 atattttgtt tccaaccaac ctgaaattat tttacgatca ttttgtgcgt cgaggttgat
     1381 gcgccattgt tcactgtgta gaaatataca ccggcgctca ggtttgaagc atcaatggtg
     1441 agggtatgga tgcctgttcc cagggttccg caattgatat tgcttacctg ttggccggaa
     1501 acattcgtta caacgatatt taccggagcc tgtttttcga ggtaaacttt tacttcagtc
     1561 gttccgctga acgggttggg aaggttctgc gatacagtaa gggctttctg tactggtttc
     1621 tcggttccgg tagggaaaac caagtgctgt atattcaggt agttgtcgcc gggctggtct
     1681 tcatcaccgc gcactgaaag gcctggttcg gtatcaagct ggtacataat tttataaccc
     1741 tccagcaaat cgcccgtggc acatattgac gggaatacac attcgtcgaa catatggata
     1801 tcatcgccgg taaggtcgaa aaaacagccg aagtcatcgc cgtaattgta aatagccctt
     1861 ccccagagat gcctgtaatc ctgtgcatcg ttgtcgaaac cctcggtgat ggcagaaaat
     1921 ataaggaatc catctccatc atcatcaatt aaagcctgcg gcatgccgat aggagagcag
     1981 taatataagc cggcttcacc aagaacattc catagaccgt cgccattaag gtcaagcgga
     2041 taagctacca gttggccggt taaatccagg gtgtcaggat taaggcagtt cagttggtat
     2101 tcatcggtgc cgccggtcca ggtgggcata ttttcattcc agtaagcaat gctgccgata
     2161 tatggatacc aggtgccttc agccgggttg gtacgataaa cgccgaagaa aagatgaact
     2221 ttgccgttga cgtcaatcac cgggtggcca ccgccatcct gtgaatacat tgagtcggct
     2281 ccggctgtga tagcggcgta aggacgcgcc caaatgatgg ttttcgtcca ggattcgccg
     2341 ttatcggtcg atttcatgag gatcaggtcc tggctgtcat cggtaacaac aaaagcaagg
     2401 gtgttgccgc gtggttcagc aaatgaatag ttgtcgccgc taaaaccgga atagagttca
     2461 gaacccaacc cgggaagaac ttccgatttg tcccaggtag cgccgccgtc ctgcgaacgc
     2521 atatagagca atgcgccgtc ctgcccgttg taggcagttc cgccattggc agtggggctg
     2581 gtgagcacta tgatgtgaat tgtgttttta tcggttccgc cggtaaccat gcggggccat
     2641 aataatccgg ctgctc
//
LOCUS       DAOPYV010000133         2447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_61774_length_2447_cov_13.833612, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000133 DAOPYV010000000
VERSION     DAOPYV010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61774_length_2447_cov_13.833612"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1086
                     /locus_tag="PKI35_11515"
     CDS             229..1086
                     /locus_tag="PKI35_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX45054.1"
                     /translation="MKRLIDNPGILITLIMFIAAFSAGNYASAQEKEGSVPDGKSSIS
                     IHISKDDNGKMIKFDTTFEAGSDFDVDAFLDEKGIGMKQPHPDADRDFEMFNFDFPEM
                     GEPFTQFPDSLLNDSLMFRLQRRWEDLSESFPKCPDMEGDFRIEAPGQGQCPRRSDAD
                     RKNNKMPGCCPLYHDNPQGMLFPQFPGMDLAPFIGYGHPEKIIVHKKRHGKKVVITFN
                     DDMESYMDDAMAIPGNFRCPSMEQHHRYIKTRPGHREQMEMKVKKSKPAQKDKQVIII
                     EKRSEEEQE"
     gene            complement(1148..2260)
                     /locus_tag="PKI35_11520"
     CDS             complement(1148..2260)
                     /locus_tag="PKI35_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNX45055.1"
                     /translation="MKKILFLGMLSALVMWGCKHEKQPGYTINGTVTGADTGWVLLKT
                     RDGYDMKTIDSAAVTEGKFVLTGTLEMPEMYYLMLKGKEGFVNFFLENSTMTATLYAD
                     SIPKSKVAGSPSQELFLSYKAKEKEFETLMENEYTRYQEAEAANDTAAQRRAENRYDS
                     VQGLQTAFQKNFILKNGKSVVAPYLAMSNAYLYSLQEFEEIDKALDTSLAKSKYVVNL
                     KERISTLTAVQPGKIAPDFSLNDTAGKPVSLASFRGKVLLVDFWASWCSPCRHENPNV
                     VAAYKKFNSKGFDVLGVSLDGEKGPWLEAIRKDGLTWTQVSDLQRWNNKASNLYGVMS
                     IPANFLLDKEGKIVASNLRGEALQLKIAELLAPPAK"
BASE COUNT          642 a          591 c          564 g          650 t
ORIGIN      
        1 actaaagtgc ctgcaataaa ttgcagggtt ttaaccagtt aataagataa taaaactcaa
       61 aattagaaat tcgtttgaga aatggccgga ttaaaagagc taaaaccgtt cctgtttcaa
      121 cccggttttg ttaacgttta ttaacacttc ttaacgtttt tgatcatcca tcggcctgat
      181 ttggtgataa ctttgataac cgaaagtaaa cattaaaaca accaaaaaat gaaacgcctt
      241 atcgataatc cgggcatcct cattaccctg attatgttca ttgctgcttt ttcggccgga
      301 aattatgctt cggcgcagga aaaggaaggc tccgtacctg acggtaaatc gtcaatatcc
      361 atccatatct cgaaagatga taacgggaaa atgataaaat ttgatacaac ttttgaggct
      421 ggcagcgatt ttgacgtcga cgcttttctg gacgaaaaag gcatcggcat gaaacagccc
      481 catccggatg cagaccgcga ttttgaaatg ttcaattttg atttcccgga aatgggcgaa
      541 ccatttacac agttcccgga cagtctgctg aatgattcgc tgatgttccg tttgcagcgc
      601 aggtgggaag acctttctga aagcttccca aaatgcccgg atatggaggg ggatttcagg
      661 atcgaagcgc ccggtcaggg tcagtgtccg cgccgttcgg acgcagaccg taaaaacaac
      721 aaaatgccgg gttgctgccc tttataccat gacaatcctc agggcatgtt gtttccgcaa
      781 ttcccgggaa tggatctggc gccttttatc ggatacggcc atcctgagaa aataatagtc
      841 cataaaaaga ggcacggaaa aaaagtggtg atcaccttca acgacgacat ggaaagttac
      901 atggatgacg ccatggccat ccccggcaat tttcgttgcc ccagcatgga acaacaccac
      961 cgatatataa aaaccagacc cggccacagg gaacagatgg aaatgaaggt caagaaaagc
     1021 aaacccgccc aaaaagacaa acaggttatt atcatcgaaa agaggtcaga agaggaacag
     1081 gagtaacatc ctgaataaaa aagcccggtt ggttactctt ccgggctttt tcttatccgg
     1141 ggcaatacta ttttgccgga ggcgcaagca attcggcgat ttttaactgc aatgcttcac
     1201 cgcgcaggtt cgacgctacg attttccctt ctttatccag aaggaaattc gcgggtatcg
     1261 acattacgcc ataaaggtta gaagccttat tattccatcg ctgcaaatcg ctgacttgcg
     1321 tccaggtaag cccgtccttc cggatggcct caagccaggg gcctttttca ccgtcaagcg
     1381 aaacgccgag gacatcaaag cctttgctgt taaacttctt atacgccgcc accacattcg
     1441 gattttcatg ccggcacggg ctgcaccagg aggcccagaa atcgaccagc agtacttttc
     1501 ctctgaacga ggcgagcgaa accggcttcc cggcggtatc gttcaggctg aaatcagggg
     1561 caattttgcc gggctgcacg gccgtcaatg tgctgatgcg ctccttcagg ttcacaacat
     1621 atttcgattt agccagcgac gtatcaaggg ctttgtctat ttcttcaaat tcctgaagac
     1681 tgtacagata ggcattgctc atggccaggt agggcgcaac gacgctttta ccgttcttaa
     1741 ggataaagtt cttctgaaaa gccgtctgga gtccctgaac cgaatcgtag cggttttcgg
     1801 ctctcctctg cgcagcggtg tcattggcag cctctgcttc ctggtaacgc gtgtattcat
     1861 tctccatcag cgtttcaaac tctttctcct tggccttgta actcagaaac aattcctgcg
     1921 acggcgaacc ggcgacctta gatttcggga tgctgtcggc ataaagggta gccgtcatcg
     1981 ttgaattttc gaggaagaaa ttcacaaatc cttctttccc tttcagcatg aggtagtaca
     2041 tttccggcat ttccaatgtg ccggtaagga caaatttccc ttcggtaacg gctgccgagt
     2101 ctatggtttt catgtcgtaa ccatcgcgcg tttttagaag tacccagccg gtatcggctc
     2161 cggtaacggt cccgttgatg gtatatccgg gctgtttttc gtgtttgcag ccccacatga
     2221 ctaaggcgga cagcatgccg agaaaaagaa tttttttcat tttgatcatt ttggtggtta
     2281 tggaaatagt ggaccctgta aaattaggaa ttatacccta accccaaaat atccttttgg
     2341 ctgttggctt ttagccatta gcctgccgtc ttacccacaa ccttgcccag gcaggtaaac
     2401 ttttagcgtt tagcgttttg tgttctgtat tctgcgttct gcgttct
//
LOCUS       DAOPYV010000134         2269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_69443_length_2269_cov_12.213640, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000134 DAOPYV010000000
VERSION     DAOPYV010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69443_length_2269_cov_12.213640"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..591)
                     /locus_tag="PKI35_11525"
     CDS             complement(121..591)
                     /locus_tag="PKI35_11525"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HNX45056.1"
                     /translation="MVRKSLLEPAFEPVDLLVGITTALRDYRAAYFLNKALDIDLKAV
                     DDLPVYHEKLKHLVRFPLFWFHQKESRTNFYLTANNNGEMLLLSALRNFNYFLIVKSD
                     APHDQFKDIPRLLREVPGIQAAFPIVPESVKNISYILSDLELHMIDVSKDDILQ"
     gene            complement(628..2055)
                     /gene="pyk"
                     /locus_tag="PKI35_11530"
     CDS             complement(628..2055)
                     /gene="pyk"
                     /locus_tag="PKI35_11530"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNX45057.1"
                     /translation="MKTKIVATLGPASSSPEMLRQLMLAGVNVFRLNFSHSSHGSHRE
                     AIDNIRALNIELNLHTAILADLQGPKIRIGIMENGQAQLPEGHRLIFTNKECTCTADR
                     LYITYPDFAADVKPGEDIILDDGKLLMRVIETNGADEVKAEVIHGGILSSKKGVNLPN
                     TRVSMPSLTAKDLTDLQFALHNRVNWIALSFVRRAQDILQLREEIARSVPEGYSPKII
                     AKIEKPEAVENMDEIIALSDGIMVARGDLGVELPLEQVPLVQKTLVNKCLAAAKPVII
                     ATQMMEGMISNIRPTRAEVNDVANSVLDGADALMLSGETSVGMFPVQVVGTMKKIIGE
                     VEEYQDVYNKRHMPDETGKERLISDAVLYSACNIACESNARAIIAMTHTGYSAGRISS
                     QRPKQDIYIFSNNHELLCMLNLLWGVNGIFYDNIVTSTSKTLRALTDKLIDDGLIKPG
                     DLLVFAASTPIYVSGKTNMLKLAYA"
BASE COUNT          533 a          528 c          564 g          644 t
ORIGIN      
        1 aaaatggaca aaggattaat ctcgtccata cggattaaag gatggcgtta acaaaatttt
       61 cgatggggag cggctacttc cgaggccgca cgcaggaagg aaagcactcc ggcaaccgga
      121 tcactgcaga atgtcgtctt ttgaaacatc aatcatatgc agttcaagat cggaaaggat
      181 ataagaaata ttttttaccg attcgggtac aatcgggaat gctgcctgta taccgggaac
      241 ttcccgcaac aatcttggga tgtccttaaa ctggtcgtga ggggcatcgc tctttacaat
      301 aagaaaatag ttaaaattcc gcaaggctga aagcagaagc atttccccgt tattgttggc
      361 tgtcagataa aaattggtgc gcgactcctt ctggtgaaac cagaacaacg ggaaacggac
      421 caggtgttta agcttttcat gataaaccgg caggtcatca acggctttca aatcgatatc
      481 cagggcttta ttcaggaaat aggcggccct gtaatcgcga agggcggttg tgatgccgac
      541 cagcagatca acgggctcaa aggccggttc gagaagggac ttcctgacca tggggcagat
      601 ttaaaatttc ctttttttca gtatgtatca ggcgtaagcc agttttaaca tatttgtttt
      661 acccgataca tagatgggtg tacttgcagc aaataccagc aaatcgcccg gttttatcaa
      721 tccgtcatca atcagtttat cggtcaaagc cctgagcgtt ttgctggtcg aagttacgat
      781 attatcataa aatatgccat tgacacccca caacaggttt aacatgcaaa gcagttcatg
      841 gttgtttgag aaaatataaa tatcctgttt gggccgttgg ctgctgatac ggccggccga
      901 gtagcccgta tgcgtcatgg cgatgatggc acgggcattc gattcgcagg ctatgttgca
      961 ggccgaatac aatacggcat ccgaaatcag gcgttcttta ccggtttcgt ccggcatatg
     1021 gcgtttgtta taaacatcct ggtactcttc cacctcgccg ataatctttt tcatggtgcc
     1081 gacaacctgt accggaaaca tgcctactga ggtttcaccg ctgagcatca gggcgtcggc
     1141 gccgtcgagc accgaattgg ctacatcgtt tacttcggcc cgggtagggc ggatgttcga
     1201 aatcatgcct tccatcatct gtgtggcgat gatgacaggc tttgcagccg ccagacattt
     1261 attcaccagc gttttctgaa ccagcgggac ctgttcgagc ggaagttcca cgcccaggtc
     1321 gccgcgggca accatgatgc cgtcggacag ggcaatgatt tcatccatat tttccacagc
     1381 ctccggcttt tcgatttttg caatgatttt aggtgaatac ccttccggta cgctccgggc
     1441 aatttcttcc ctgagctgaa ggatatcctg ggcccggcgt acaaacgaga gagcgatcca
     1501 attgaccctg ttgtgcaacg caaattgcag gtcggtcagg tcctttgctg taagcgaagg
     1561 catcgaaacc cgggtattcg ggaggtttac cccctttttt gacgataata tcccgccatg
     1621 tattacttcg gcttttactt cgtcagcgcc attggtttct ataacccgca tgagtaactt
     1681 gccgtcatcc aggatgatat cctcgcccgg ttttacatcg gctgcaaaat ccggataagt
     1741 tatgtatagc cggtcggcag tgcaggtaca ttctttattt gtaaaaatca gccggtgccc
     1801 ttcgggaagt tgcgcctggc cgttttccat gatcccgata cgtattttcg gtccctgaag
     1861 gtcggccagg attgcggtat gaagattcag ttcaatattc agggccctga tattatcgat
     1921 ggcctcgcgg tgcgatccgt gcgagctgtg tgagaaattc agcctgaaaa cattaacgcc
     1981 ggcaagcatg agttgcctta acatttcagg cgacgaggat gccggaccca gcgttgctac
     2041 aatttttgtc ttcatagagt attatgctat atggttaccg atgatttatt ttcatttttt
     2101 ccggtcagca gcagtcctga accggctctc tttgccagca ttttaagttt tgcgttttgc
     2161 gttttgcgtt ctgtgttctg tgtttcgtga ttcgtgattc gttgtcagtg tttcagtgtt
     2221 tcaaggtttg ggtttaagcc tttgcgtttt gcgttttgcg ttctgtgtt
//
LOCUS       DAOPYV010000135         2264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_69695_length_2264_cov_14.719783, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000135 DAOPYV010000000
VERSION     DAOPYV010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69695_length_2264_cov_14.719783"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..>2264
                     /locus_tag="PKI35_11535"
     CDS             58..>2264
                     /locus_tag="PKI35_11535"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNX45058.1"
                     /translation="MKTASTGNSASTVFFMKGQRNLQTSNQPLFVVDGVPIAGEFLSA
                     LGLDYGNLVNDWNLDDVESVTVLKSGQAAALYGNLAGNGAVIINTKRADERGFHVDYN
                     SAAMIRGIADYPEFQNEYGQGWAGLFSYEDGEGGGLNDGGDCSWGPKFEGQLISQFDG
                     PSTGWIDGQAVEVRGGDVWARRQAALNGIDNSIEPTPWVSRPDNMKDYFQTAFLLSNN
                     LGISWADKNGGIRVSYTNTRANDVTPCSRFTKNSLNGNASYTFFKRINVYGSFLSTKM
                     MDENVIIQDYEESGSPMEFFAWMGRQVNVNSLKNYWQAGRRDSTQYNYNGTYWDNPWF
                     RDKERVSDVGRTNLFGTLGAKISITRRFSLNYVLGSYDIDANGKHVVPPASVDITCEK
                     NEASGLSGTRHSVFADWNFRLGKANFFDFFLGWYSYNTKSSLNEREMLNGHDWLLEYN
                     TTDKKHGCYGGVSFSCKEALHLRLTMTRDNDDLWSDEQHQALPVYYAASTGADVEKIL
                     RLPEVVSRFSIQTGYSRTGLNGTFQYSFINGERMNVPVISEYTISAELGLFSNRVTAS
                     FNGYSSRTINIDNLMGIYATAFDDAAMKNSGVEIGLDVIPVRLRKVTWESSVAFFRNK
                     NILQDFREGCYFYVTNPITLGCAGGQSYGDIYGTAFNRYNGKIVFERGYATKASVQSL
                     GNVNPDYMIFWNNSVRINKVTITAGIEYSKGGSYYSSFYLWGTYAGTLSHTADR"
BASE COUNT          606 a          473 c          602 g          583 t
ORIGIN      
        1 gccgggtatt cccctaacat actaagctcc ctgacgggaa aagtatccgg cctcgatgtg
       61 aagacagcat ccacgggaaa ttcggcgtcg accgtctttt ttatgaaggg gcagcgaaac
      121 ctgcagacaa gcaaccagcc tttatttgtg gttgacggcg ttcccattgc aggcgaattt
      181 ttgtccgctt tggggcttga ttacgggaac ctggtcaacg actggaacct ggatgacgta
      241 gaatcggtca ccgtactgaa aagcgggcag gcggcagcct tatacggcaa cctggccggg
      301 aacggcgcag tgatcatcaa taccaaaaga gcggatgagc ggggctttca cgtcgactac
      361 aattcggcgg ctatgatcag gggaattgcc gattatcctg aatttcagaa cgaatacggc
      421 cagggctggg ctgggctttt cagctatgaa gacggtgaag ggggcggctt gaacgacggg
      481 ggtgattgca gttgggggcc aaagttcgaa gggcaactga taagccagtt cgacgggcct
      541 tcaaccggct ggatcgatgg tcaggcggtt gaagtaaggg gcggtgatgt gtgggccagg
      601 cggcaggctg ccctaaacgg tatcgataat tccatagagc ctacgccctg ggtgtcccgg
      661 ccggataata tgaaggatta tttccaaacg gcttttttac tttcaaataa cctggggatt
      721 tcctgggctg ataaaaacgg cgggatcagg gtttcctata ccaacaccag ggccaacgat
      781 gtgacgccct gcagccgttt cacgaaaaac tcgctgaacg ggaacgcctc ctatactttt
      841 ttcaaaagga tcaacgtgta tggttccttt ttaagcacga agatgatgga tgaaaatgta
      901 attattcagg attatgaaga atccggttcc ccgatggaat tctttgcctg gatgggcagg
      961 caggtgaatg tcaacagcct gaaaaattac tggcaggccg gccggcgcga ttcaacgcaa
     1021 tataactaca atggtacata ctgggataat ccgtggttca gggacaaaga aagagtatct
     1081 gacgtgggaa ggacaaacct ctttggtacg ttaggggcaa aaatttccat caccaggaga
     1141 ttctctttga attatgtgtt gggatcctat gatattgatg cgaacggaaa acatgttgtt
     1201 ccgccggctt ccgttgatat cacctgtgag aaaaatgaag cgtcgggcct ttccggcacg
     1261 cggcattccg tgtttgccga ttggaatttt cgcctgggga aagcaaactt tttcgatttt
     1321 ttcctgggct ggtattccta taacacgaaa agcagtttaa atgaaagaga aatgcttaac
     1381 ggccatgatt ggcttctgga atataacact accgataaaa agcatggctg ttacggcggt
     1441 gtatcctttt cgtgcaagga ggctttgcat cttcgtctta ccatgaccag ggataatgat
     1501 gatctctggt ctgatgagca acatcaggca ttgccggttt attacgctgc atcgacgggc
     1561 gctgatgttg aaaagatact tcgtctgcct gaagttgttt ccaggttcag catacaaaca
     1621 ggatactcac gaacagggtt aaacggcaca ttccagtatt cattcattaa tggtgaaaga
     1681 atgaatgtgc ctgttatttc cgaatatacc ataagtgcag aattggggct gttttcaaac
     1741 agggtaacgg catcttttaa cggttattct tcacggacaa taaatatcga taatttgatg
     1801 gggatttacg ctacagcatt tgatgatgct gcaatgaaaa attcaggagt ggaaatcggc
     1861 ctcgatgtaa ttccggtacg actacggaaa gttacctggg aatcttctgt cgcatttttt
     1921 agaaataaaa atatactcca ggatttccgg gagggttgtt atttctatgt aaccaatccg
     1981 attacactcg gctgtgctgg tgggcaatcc tatggagata tttatggaac tgcattcaac
     2041 cggtataacg gaaaaatagt cttcgaaagg gggtatgcta caaaggcaag cgttcagtca
     2101 cttggtaatg tgaaccccga ttacatgatt ttttggaata attcggtcag aatcaacaag
     2161 gtcacaatta cggcaggtat agaatatagc aaagggggca gctattattc ttcgttctat
     2221 ctatggggaa cctatgccgg gacgctcagc catacggctg accg
//
LOCUS       DAOPYV010000136         2222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_71710_length_2222_cov_12.411629, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000136 DAOPYV010000000
VERSION     DAOPYV010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71710_length_2222_cov_12.411629"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2222)
                     /locus_tag="PKI35_11540"
     CDS             complement(<1..>2222)
                     /locus_tag="PKI35_11540"
                     /inference="COORDINATES: protein motif:HMM:NF040095.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HNX45059.1"
                     /translation="MESEPTIKTISHSGRLDTNTSADLDLALQPLLESGQFIILDLLQ
                     CPYMSSIGIRVLLKTKKKLLAKQGDLYLTGLVPAVFQVIEMAGLHNIFQIKENPEAAR
                     EAIRTIQKNSMGIMTCVSEGISYVYHPLNGKAQPALLWKDDGIASYNELGFSIGFGAP
                     AESGIDDQEQSGLFVTTGHCAGFIPDDPHHAADFRVSPEPEKAGIMLKEALSFGRQPS
                     GLLRMKEPGRVSFQQLADASETLKKQLSPEKTGWMLMVAVNPNPAEPTISLILPAGKP
                     LHDFARMEDFQRWFSTGGNNGHLPGVVFSLSELQNADAEKPLVQLLNENLTIENIVDI
                     QPLQKQENLENPWVWVFFSNDLSDAKAHRLIIETDPEMIFEPYKAFLVRRLYTDSAKV
                     KVDPLHGGYTAQTYQVTSYDAEGRKMRPTVLKVANRAMITRESERCRQYALPYIFNNS
                     AVVLGAEFYGETGALRYNFVGIGGEAAQLKWLTHYYNNAEMELLEPLFDKVFLQILKP
                     WYGQPVQKIIFPFKDHDPTFTFFPYIYQTVDELFSISADEKYITVEEFDQPILNPYWF
                     LKHEFARRRDEGMDYFTGICHGDLNMQNILLDEKMNVYLIDFSETKPRSVISDFARLE
                     AIFLVDNAPVDNETDMKDYLQFITLFYDVKHLNEIPVANYAGRHREKVAKSTALTLKM
                     RKYAFDCAQQNPDPIPYYLALLEWVLPVVCYGLPIPQRRVSMIVSSILCRKVMDAENG
                     "
BASE COUNT          578 a          505 c          531 g          608 t
ORIGIN      
        1 tttccgtttt cagcatccat cactttccgg cacaatatcg aagatacaat catcgaaaca
       61 cgccgttgag ggatgggtag gccatagcaa acgacaggaa gcacccattc gagcaaggca
      121 agataataag gtattggatc gggattttgc tgagcgcagt cgaaagcata ttttctcatt
      181 ttcagggtaa gagcggtgct tttggctact ttttcgcgat gcctgcccgc atagtttgct
      241 acaggaattt cgttcagatg tttaacatca taaaacaggg tgataaactg tagataatcc
      301 ttcatatcgg tttcgttgtc caccggagca ttgtcgacca ggaaaatggc ttccaaacgg
      361 gcaaagtcgg agatgaccga acggggtttg gtttccgaaa agtcaatgag atagacattc
      421 attttttcat ccagcaggat gttttgcatg ttcaggtcgc catggcagat accggtgaaa
      481 tagtccatgc cctcatcgcg gcgtctggca aattcatgtt tcagaaacca gtacggattc
      541 aggatgggtt ggtcaaattc ttcaaccgta atgtattttt catccgccga aatggagaac
      601 aattcatcga ccgtttgata aatatatggg aaaaaagtga aggtggggtc gtgatccttg
      661 aaagggaaaa tgattttttg taccggctgc ccgtaccagg gctttaatat ctgcaggaat
      721 actttatcga ataacggttc cagcagttcc atctctgcat tattgtaata atgggtcagc
      781 cattttaact gagcggcttc gccgccgatg cccacaaaat tatacctcag ggcgcctgtt
      841 tccccataaa attctgcgcc aagcactacc gcgctgttgt tgaaaatata aggaagagca
      901 tattgcctgc aacgctccga ttcgcgggta atcatggcac ggttggcaac tttaagcacg
      961 gtaggcctca ttttcctgcc ttcggcatcg taggaggtaa cctggtaagt ctgcgccgta
     1021 tagcctccat gtaacggatc cacttttacc ttagccgaat cggtatatag ccgtcgcacc
     1081 aaaaatgctt tgtaaggttc aaaaatcatt tccggatcgg tttcaatgat caggcgatgg
     1141 gctttggcat ccgataaatc gttggagaaa aatacccata cccagggatt ttccagattt
     1201 tcctgctttt gcaaaggctg aatatcaacg atattttcaa tggtcagatt ctcgttgagc
     1261 aattgaacca atggtttttc tgcatccgca ttttgcaatt ccgaaaggga aaaaacaact
     1321 cccggcagat gtccgttgtt tccgccggta gaaaaccatc tttgaaaatc ttccatccgg
     1381 gcgaaatcgt gcagcggttt accggcaggt aatatcaagg aaatggttgg ttctgcagga
     1441 ttggggttga cagccaccat cagcatccat ccggtttttt ccggcgaaag ctgttttttc
     1501 agcgtttcgg aagcgtcggc taattgctga aacgaaaccc tgccgggttc tttcatccgt
     1561 aacaaacccg aaggctgtct accgaaggat aaagcttctt ttagcatgat gcctgctttt
     1621 tccggttccg gtgaaaccct gaaatcggcc gcatgatgag gatcgtcggg gataaaaccg
     1681 gcgcagtggc cggtagtaac aaataaaccc gattgctcct gatcgtcaat gcccgattcg
     1741 gcgggagcac caaatccgat ggaaaagccc aattcattgt aactggctat gccatcgtcc
     1801 ttccataaca atgcgggttg ggctttaccg tttaacggat gataaacata cgaaatgcct
     1861 tccgaaacac atgtcatgat tcccatagaa tttttctgaa tcgtacggat ggcttcgcgg
     1921 gcggcttccg ggttttcctt aatctgaaaa atgttatgca gtccggccat ttctattacc
     1981 tgaaatacgg caggcactaa gcccgtgaga tataaatcgc cctgcttggc aagcagtttt
     2041 ttcttggttt tgagcagaac gcgtatgcct atactcgaca tatagggaca ttgcaaaaga
     2101 tcaaggatga taaactgccc gctttcgagc aagggttgaa gcgccagatc aaggtctgcg
     2161 ctggtattgg tgtcgaggcg acccgaatgg cttatggttt ttatcgttgg ttccgattcc
     2221 at
//
LOCUS       DAOPYV010000137         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_85643_length_1978_cov_17.373895, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000137 DAOPYV010000000
VERSION     DAOPYV010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85643_length_1978_cov_17.373895"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PKI35_11545"
     CDS             <1..866
                     /locus_tag="PKI35_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNX45060.1"
                     /translation="AGKEDKYYREIIEHLNSLKGVDGWFTENYGADQAEKKKPFQLKN
                     TRKLDAIRQEIEQLNLEWVDKCVILTSLIIALDKVDNTLGHYAAYLSDWSKRSYKDLT
                     LKLPKRFPITSENKVVRGDIFDTIGKKEYDLAYFDPPYGSNNEKMPPSRVRYSAYYHI
                     WTSIIQNDTPALFGKANRREDTRDTQAGSVFEEFRKDENGHFIAMQSLRRLIEETRAR
                     YFLLSYSSGGRATKEELHQAMTENGKLLKVVEIDYTKNVMANMRWTNEWINSDGKHRE
                     YLFLMEKTPNP"
     gene            934..>1978
                     /gene="thrC"
                     /locus_tag="PKI35_11550"
     CDS             934..>1978
                     /gene="thrC"
                     /locus_tag="PKI35_11550"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNX45061.1"
                     /translation="MIKSSQKEAPNSPLGVGGRYYSTNNLSLTVSLQEAVVKGLAEDK
                     GLFMPEKIEKLPQEFFDTIGTLSFPEIAYTVAEAFFGSDIEADELKKIVNETLSFDTP
                     LVEVEDRIYSLELFHGPTLAFKDVGARFMSRMLAYFISKQQNTEPVNVLVATSGDTGS
                     AVANGFLGVEGIRVFVLYPKGLVSKIQESQFTTLGQNITALEVDGTFDDCQRLVKSAF
                     LDEELNRKMRLTSANSINVARFLPQAFYYFYAFAQLRSMQPKRSAEELETVFCVPSGN
                     FGNLTAGLIAKRMGLPVKRYIAANNRNDIFLQYLKSGIYTPKPSVATIANAMDVGDPS
                     NFVRVLDLYGHSHE"
BASE COUNT          578 a          438 c          491 g          471 t
ORIGIN      
        1 aagccggtaa agaggataag tattatcggg aaattattga acacctgaat agtttgaaag
       61 gtgttgatgg ctggtttacc gaaaactacg gcgcagacca ggctgaaaag aaaaaacctt
      121 ttcagctaaa aaacacccga aaattagatg ctatccgaca ggagattgag caattgaatt
      181 tggaatgggt agataaatgt gtgattttaa ccagtttgat tattgcgctg gataaggtgg
      241 acaatacgct ggggcattac gccgcttacc tgagcgattg gtcaaaacgg tcatataagg
      301 atttgacgct gaaattgccg aaaagatttc cgataacgag tgaaaataag gtagttcgcg
      361 gtgatatttt cgacacgatt gggaaaaaag aatacgattt agcctatttc gatccgcctt
      421 acggatctaa taacgagaaa atgcctccca gccgcgtgcg ctattcggca tattatcaca
      481 tctggacttc gataattcag aacgatacac cggccctttt cggcaaggct aaccgccgcg
      541 aagacacccg cgatactcag gcgggatcgg tttttgagga attccggaaa gatgaaaacg
      601 gacacttcat agccatgcag tcgctgagaa gattaataga ggaaaccagg gcaaggtatt
      661 ttttgctttc gtacagttcg ggcggacgtg cgacaaaaga ggaactgcat caggccatga
      721 ccgaaaacgg caaactactg aaagtcgtgg agattgatta caccaaaaac gtgatggcca
      781 acatgcgctg gaccaacgag tggatcaaca gcgacggaaa acaccgggag tatttgtttc
      841 taatggagaa gacccccaac ccctaaaggg gagttttcat gaggtcacaa aagatttagt
      901 acatttaatg tttatgataa gaaattgtcg aatatgatca aatcatcgca aaaagaagct
      961 ccaaactccc ctttaggggt tgggggccgg tattacagca ccaacaacct atcactcacc
     1021 gtttcgctgc aggaagctgt cgttaaaggg ctggccgaag ataaaggcct gtttatgccc
     1081 gaaaaaattg agaaattacc gcaggagttt ttcgatacca tcggtaccct ttctttcccc
     1141 gaaatagctt acacagtggc tgaggccttt tttggcagtg acattgaagc agatgagctg
     1201 aaaaaaatcg tgaacgaaac gctcagtttt gatacaccgc tggtggaagt ggaagacaga
     1261 atatacagcc tcgaactttt ccacgggcct acattggctt tcaaggatgt aggagcgcgg
     1321 tttatgtcgc gtatgctggc ctatttcatc agcaaacaac aaaacactga accagttaat
     1381 gtgctggtag ccacttcggg cgatacggga agtgccgtgg ccaacggatt tctgggtgtg
     1441 gaaggcatcc gcgtgtttgt tctttacccc aaaggactgg taagtaaaat tcaggaaagt
     1501 cagtttacca cgctcgggca gaatatcact gcgctggaag tggacggaac tttcgacgat
     1561 tgccagcggc tggttaaatc ggcctttctg gacgaggaac tgaaccggaa aatgcggctt
     1621 acttcggcca attccatcaa tgtagcccga tttctgccgc aggcatttta ctatttttac
     1681 gcattcgctc agctccgctc catgcagccg aaacgttcgg ccgaagaact ggaaaccgtg
     1741 ttttgcgtac cgagcggcaa ctttggcaac cttacggccg gtctgattgc caaacgtatg
     1801 ggactgcccg tgaaacgata tatagctgcc aacaaccgca acgacatttt tctgcaatac
     1861 ctgaaatcag gcatttatac cccaaaacca tcggtagcca ccattgccaa tgccatggac
     1921 gtaggcgatc cgagcaattt tgtccgcgtg ctggatttgt acggacattc gcacgaag
//
LOCUS       DAOPYV010000138         1871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_93487_length_1871_cov_14.902533, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000138 DAOPYV010000000
VERSION     DAOPYV010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93487_length_1871_cov_14.902533"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1033)
                     /locus_tag="PKI35_11555"
     CDS             complement(122..1033)
                     /locus_tag="PKI35_11555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNX45062.1"
                     /translation="MSSGGSTKAIFFALGGNLLISAIKYIVSFVTGSAAMLAESIHST
                     ADSLNQVFLLIGNRRSKKVPDELHSFGYSNEVFFWSMMVAVLLFFVGALFSVYEGIQK
                     LSHHEQVENAMWIFIVIGSSILIEAKSFHVAYTEFRKSHKQPMLQAIRESTDVNLIVI
                     LLEDFAALVGLTIVLISTVLALFVHPVFDAIGSILVGALLLIISILLINEVKNLIIGE
                     SIPRDQRNLIKQIIHSRKEIRHINRLQTMVMGNHRYLVLISVDIDDSITGYRIEDLIE
                     KLKAEIRTKTEGIENIYIEVKDSARNQ"
     gene            complement(1201..>1871)
                     /gene="rnc"
                     /locus_tag="PKI35_11560"
     CDS             complement(1201..>1871)
                     /gene="rnc"
                     /locus_tag="PKI35_11560"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNX45063.1"
                     /translation="KNIFGFYPGNIFLYKLALRHKSVAKELNNGTKVSNERLEYLGDA
                     ILSAVVADHLFKTFPLKDEGFLTEMRSRIVSRAQLNNLSQKIGLDNLIQVNAESSNVY
                     RSMKGDAFEAMVGAIYLDQGYEFTRKVLIEKVISLHFDLEELENNNTNYKSRLIEWAQ
                     REKVNVEFVTLNEVGHGYTKQYVVEVHLDKKAYGKGRDYSIKGAEQNAAMKTLMQMEE
                     QKSA"
BASE COUNT          477 a          423 c          439 g          532 t
ORIGIN      
        1 ggcaggtaaa gttttccgtt ctccgttcac cgttttcctt tgacctttgc cctgccgtct
       61 tgcccacaac ctcactcagg caggtaaact tttaaccttt gacctttgcc tttctcaaga
      121 gttactggtt cctggcgctg tcctttactt cgatataaat attttcaatc ccttccgttt
      181 tggttctgat ctctgctttg agtttttcaa tcaaatcctc aatccggtaa ccggtaatgc
      241 tgtcgtcaat gtctaccgaa atcagtacga gataacggtg attgcccatc accatagttt
      301 gcaaacggtt gatatgcctg atctccttcc gggaatgaat gatctgtttg atcaggttcc
      361 gctggtcacg ggggatactt tcgccgatta tcaggttttt cacctcgttg atcagcagaa
      421 tggagataat cagcaggagc gctcctacca ggatgcttcc gatggcatcg aataccggat
      481 ggacaaataa tgccaggaca gtcgaaatca gaacgatggt cagaccgaca agggcggcaa
      541 aatcttcgag cagtatcacg atcaggttca cgtcggttga ttccctgatg gcctgaagca
      601 tgggctgttt atggcttttg cgaaattcgg tataggcaac gtggaacgat ttggcttcaa
      661 taaggatgga agaaccgatc acaatgaaaa tccacattgc gttttcaacc tgttcgtggt
      721 gcgagagttt ctgaatacct tcataaacgg aaaacagggc cccgacgaaa aacaacaaaa
      781 cggccaccat catcgaccag aaaaatactt cgttgctgta accaaaacta tgcagctcgt
      841 cgggcacttt tttcgatctc ctgttgccga tcagcaggaa aacctggttc aggctgtcgg
      901 ccgtggagtg aatggattcg gcaagcatag cggcgcttcc tgtgacgaac gaaacaatgt
      961 atttgatggc tgaaatcagc agattgcctc cgagggcaaa aaatatcgct ttggtcgatc
     1021 ctccggaact catgaatgaa gatatgtatt acaaaggtaa ggaagtgatt gaggcagaca
     1081 gatagcagtt gatagtttaa agaaaaccgg atagcggaag cctctgcagt ggctgcagat
     1141 ataagacatt gagaaatagc ccagaaatac ctttcggcgg ggaaacggaa atccgacgaa
     1201 tcacgctgat ttctgttctt ccatctgcat cagggttttc atggcagcat tttgttcggc
     1261 gcctttaatg gaataatcgc ggcccttgcc gtatgctttt ttatcgaggt gcacttcaac
     1321 cacgtattgt tttgtatatc cgtggcctac ttcgttgagg gttacaaatt caacgtttac
     1381 tttttcacgc tgtgcccatt caataagccg gctcttataa ttggtattgt tattctccag
     1441 ttcttcaaga tcgaaatgga ggcttatcac tttttctatc aggactttcc gggtaaattc
     1501 ataaccctgg tccaggtaaa tggcgcctac catggcttca aaagcatcgc ctttcatcga
     1561 gcggtaaaca ttgctgcttt cggcattaac ctggatgagg ttgtccaggc cgattttttg
     1621 ggagagattg ttaagctgcg cccggcttac gatgcgggaa cgcatttcgg taaggaagcc
     1681 ttcgtcttta aggggaaagg ttttaaagag atgatcggcg accacggcgc ttaagatggc
     1741 atcgcccaga tattcgaggc gttcgttact tacttttgtg ccgttattca gctctttggc
     1801 aacgctttta tggcgcaagg ccagtttata caaaaaaata tttccggggt aaaacccgaa
     1861 aatatttttt a
//
LOCUS       DAOPYV010000139         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_97742_length_1819_cov_8.978458, whole genome shotgun sequence.
ACCESSION   DAOPYV010000139 DAOPYV010000000
VERSION     DAOPYV010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97742_length_1819_cov_8.978458"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PKI35_11565"
     CDS             <1..553
                     /locus_tag="PKI35_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNX45064.1"
                     /translation="VIFSTTGYTGSGGCEIYFPNKEAVTIWNAVMAAGEEFGIKPIGL
                     AARDTLRLEMGFMLYGNDIDDTTSPLEAGLGWITKFTDEKNFIDKQFMLDLKANGLRR
                     KLVGFELIDKGVPRHGYDVVDAANQKIGVVTSGTMGPSVKKAVGMAYVPVAFSKEGSE
                     IFISVRDKALKAKVVRPPFYKPE"
     gene            770..1489
                     /locus_tag="PKI35_11570"
     CDS             770..1489
                     /locus_tag="PKI35_11570"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNX45065.1"
                     /translation="MLSIETCFSPLLFPARMMQGNHIVVVIDVLRATTAFCAAFDRGF
                     RSILPVATLEEARSYKEKGCVVAAEREGNKLDFADFGNSPVAFLRAEKVAEKLVYSTT
                     NGTRAIETGKQADLLVAASFSNLSAVSRWIENKGKNLLILCSGWKNTFSLEDAMCAGA
                     LASRLMANKNFTTSCDATRVAMFLWKNAEPGPEGFAKQSSHYQRLLNKGLSDDLNYCF
                     RMDTSGAVPVMADGVLVNAGR"
     gene            1628..>1819
                     /locus_tag="PKI35_11575"
     CDS             1628..>1819
                     /locus_tag="PKI35_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNX45066.1"
                     /translation="MTEDFLHYIWQFRLYGAGLRLATGETLDVIHPGIHNTDAGPDFF
                     NARIQIGDTVWAGNVEVHVQ"
BASE COUNT          460 a          419 c          441 g          499 t
ORIGIN      
        1 tgtgatcttc tccacgaccg gttatacggg ttcgggcgga tgcgaaatat atttccccaa
       61 caaagaagct gttaccatct ggaatgctgt tatggcggct ggtgaagaat tcggcattaa
      121 gcccatcggt ctggcagccc gcgatacact tcgcctcgaa atgggtttca tgctttacgg
      181 taatgatatt gatgatacca cttctccgct ggaagcaggc ctcggttgga tcactaaatt
      241 taccgatgag aaaaatttta tcgataaaca gtttatgctc gatctgaaag ccaatggatt
      301 gcgtcgtaaa cttgtcggtt tcgaattgat tgataaaggc gttccccgcc atggatacga
      361 tgtggttgat gccgcaaatc agaaaatagg cgttgttacc agcggtacaa tgggcccaag
      421 cgttaaaaaa gctgtcggaa tggcttatgt tccggttgcc ttctcgaaag aaggatctga
      481 aatttttatc agcgtccgcg ataaagccct gaaagcaaaa gtggtacgtc ctccttttta
      541 taaacctgaa taacagcgga atgcattata gagaaccggg cttgcacagg cagtccggtt
      601 tttttaatct ttgtacccgg tttacttccg gatgccagcc ggtaaccgac aataccggtt
      661 gtacctttgt gacagaatgg ctgcgctggg ctaaacagat gtttaaccgc atcataacaa
      721 attaatttta actgttttat gaatcaggcc gttataaatc cctaagagta tgctttcaat
      781 cgaaacctgt ttcagccccc ttcttttccc cgcgcgtatg atgcagggaa atcacattgt
      841 tgttgtgatt gatgtactca gggcgacaac ggccttctgc gccgcatttg accggggctt
      901 ccggtccatt cttcccgtgg cgacccttga ggaggcccgc agttataagg aaaaaggatg
      961 tgtggttgca gccgaacgcg agggcaacaa acttgatttt gccgacttcg ggaattcacc
     1021 tgttgctttt cttcgtgccg aaaaggtagc cgaaaagctg gtgtattcaa caaccaatgg
     1081 cacccgggcc attgaaaccg ggaaacaggc tgatttgctg gtagcggcat cgttctcaaa
     1141 cctgtcggcc gtgtcacgct ggatcgaaaa taagggcaaa aacctcctga ttctgtgctc
     1201 gggctggaaa aacacattca gcctcgaaga tgctatgtgt gccggagcgt tggcaagccg
     1261 actcatggca aataaaaact tcactacatc ctgcgatgcc acgcgtgttg ccatgttctt
     1321 gtggaaaaat gccgaacccg gcccggaagg gtttgcaaag caatcgtccc attatcagcg
     1381 cctgttaaac aaaggccttt ccgatgacct gaactattgt ttccgaatgg atacttccgg
     1441 ggctgtcccg gtaatggctg acggcgttct tgtgaatgcc ggcagataat tttcctctta
     1501 tttccggatt tggaagtgtc cattatttcc atcatataaa aattattgct gttttttttg
     1561 aaaaatcggg aacgataaat tgattgaaat caatttctta atatctttcc gatcgaaaaa
     1621 cagccctatg actgaggatt ttcttcatta catctggcaa ttcaggctct atggcgccgg
     1681 gctgcgcctt gctaccggtg aaacccttga tgtgatacac cccgggatac ataacaccga
     1741 tgccgggcct gactttttca atgcccgtat tcaaatcggg gatacggttt gggcaggtaa
     1801 tgttgaagtg catgttcag
//
LOCUS       DAOPYV010000140         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_104911_length_1739_cov_13.294537, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000140 DAOPYV010000000
VERSION     DAOPYV010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104911_length_1739_cov_13.294537"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PKI35_11580"
     CDS             complement(<1..581)
                     /locus_tag="PKI35_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HNX45067.1"
                     /translation="MAYSGPKDDRIFPETGGLPSKGDRAQFDLIFRSYIPRLLAYGEL
                     FTDRETVQDIVQDLMVYIYDHADTIVIHTSLEAYLFKAVYLRCLNHIRHSRIQRDYRE
                     HQTEDFIEHEAVYYDPDQNDIIRKIYSRELRDELMTAINELPLKCREAFIKSYIQEMN
                     AREIAAQLNISERTVNSHVYTALKHLRAKLKDKL"
     gene            785..1675
                     /locus_tag="PKI35_11585"
     CDS             785..1675
                     /locus_tag="PKI35_11585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNX45068.1"
                     /translation="MEHTHGQAHAGYSGKAFRTGIAINMVFIALEVFYGLLSNSMALV
                     SDAGHNFSDVAALAFSWLAIRLSERKPTIKFTYGFRRSTILAAVLNTMLLFAAAAFLL
                     VETIDRLSAPAAINSGNVVVVATAGILVNGFTAWLFTRGRKHDLNIRSAFVHFLADTL
                     VSAGVVLAGIIMYFTGITWIDAVVSFAIIVVILYSSFRLLIDSVSLALDAVPEFVDIG
                     AVREYLSSLPEVTGIHDLHIWALSTNAAALTVHLETKVAAGNLSMPDIQRNLHDLFNI
                     EHSTIQVEPGGMPEHCNHCN"
BASE COUNT          391 a          416 c          469 g          463 t
ORIGIN      
        1 agtttgtctt tcagtttggc gcggaggtgc ttcagggccg tatacacatg cgagttgacc
       61 gtgcgctccg agatgttcag ttgcgctgca atttcccgtg cattcatctc ctggatatag
      121 ctcttgataa aggcttccct gcatttgagg ggtaattcgt tgatggccgt catcagttca
      181 tccctgagtt cccggctgta aatcttcctg atgatgtcgt tctgatcggg atcgtaataa
      241 acggcctcat gctcaatgaa atcctcagtc tggtgttcac ggtaatcccg ctgaatccgg
      301 ctgtgccgga tatgattcag gcagcgcagg tataccgctt tgaaaagata ggcttcgagc
      361 gacgtatgaa tgacgatggt atcggcatga tcgtaaatgt aaaccatcag gtcctgaaca
      421 atgtcctgta cggtttccct gtcggtgaac aactcaccgt aagccagcag acgcgggatg
      481 taggaacgga atataagatc aaactgcgcc ctgtcaccct ttgaggggag cccgcctgtt
      541 tcgggaaaaa tccggtcgtc ttttggtcca gagtaagcca agttggtttt gcgagtattt
      601 gggcagttta aacggtgctt ctgatttcta caaatttagg aaactgggtt aaacagagga
      661 tcgaaaaaaa aggaaatctt cgtttgccga catccggtca tcctcctgca acctgtagcg
      721 gggagaaaat gaaccggaac gaaaagtctt tgttgtacat ttacaccggt taaaataatt
      781 ggttttggaa catacacacg ggcaggcgca cgccggatat tccggaaagg ctttcaggac
      841 agggattgcg atcaatatgg tttttattgc cctggaagtt ttctacggat tgctctccaa
      901 ttcgatggcg ctggtatccg atgccgggca taatttcagc gacgtggcgg ccctggcttt
      961 ttcctggctg gctatccggc tgtcggaaag gaagccgact ataaaattca cctatgggtt
     1021 caggcgttcc accatactgg ccgccgtgct gaataccatg cttttgtttg ccgcggctgc
     1081 atttctgctg gtagaaacca tagaccgcct atcggcgccg gctgcgataa attccggaaa
     1141 tgttgttgtg gtggcaactg ccggaatcct ggtcaacggg ttcacggcgt ggcttttcac
     1201 caggggcagg aaacacgacc tgaatatccg aagcgctttt gttcattttc tggccgacac
     1261 gctggtttca gccggcgttg tgctggccgg tattatcatg tattttacag ggattacctg
     1321 gatcgacgcg gtggtgtcgt ttgccatcat cgttgtgatc ctgtacagtt cgttccggct
     1381 gctgatcgat tcggtaagcc tggcccttga tgctgttccc gaatttgtgg atatcggcgc
     1441 cgtccgggaa tatctcagca gcctcccgga ggtgacaggc attcatgacc ttcatatctg
     1501 ggcgctgagc accaatgcgg cggcgctcac cgtacacctg gagacaaaag ttgcggcagg
     1561 aaatttgtcc atgccggata tccaacgaaa cttgcacgat ctgttcaata ttgagcattc
     1621 aaccatccag gttgaacccg gcggaatgcc ggaacattgt aatcattgca actaagcggc
     1681 cgtaagcgga ccagggattg gaaccaacgg aggtggttat tccggatatt atcttcctg
//
LOCUS       DAOPYV010000141         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_110758_length_1680_cov_11.062154, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000141 DAOPYV010000000
VERSION     DAOPYV010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110758_length_1680_cov_11.062154"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKI35_11590"
     CDS             <1..576
                     /locus_tag="PKI35_11590"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNX45069.1"
                     /translation="GDFTFSLLVLSAAVMKADEKVMKSELDYVKRFLVNQFGVIQAEQ
                     HLHMLRNILTQELDVMAVSAQIRQYMDYSSRLQLLHYLFGIASADGGFNLKEIGMAVQ
                     IAQLLGIRQQDIESVKAMFVRDTQSAYKILEIRPDATDEDVKKAYRKMALKYHPDRVA
                     HLGEDVQKAANAKFQELNAAYESVKKERGMV"
     gene            complement(761..1591)
                     /locus_tag="PKI35_11595"
     CDS             complement(761..1591)
                     /locus_tag="PKI35_11595"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HNX45070.1"
                     /translation="MGITIYIFILILLAAGVLLITHFGPAFVRIEKSGLSFFSQKDTE
                     SLLPASQVEMINKASSLYPLLNNKLKRRFERRLATFMTRKNFVASEGIDTITEEMKVI
                     ISAIFTQLTFGHPAQYLDMFKTVILRADDYYSSRTGQYYEGEVNLIGCIVLSWKNLMA
                     DLENESDGHNLGLHEAAHALRLSYAFFEERESDEMTKLIHEFDELAVQEMNNYETGNQ
                     TFFREYGAENYNEFFAVAIECYFEKPRQLFEYNPLIYNKLKQILKLDLLDPEHIIAKE
                     "
BASE COUNT          426 a          372 c          403 g          479 t
ORIGIN      
        1 ggtgatttta ctttcagcct gctggtgttg tcggcagccg tgatgaaagc cgatgaaaaa
       61 gtaatgaaat cggaactgga ttacgtgaaa cgtttccttg tcaaccagtt tggagtaatt
      121 caggctgagc agcacctcca catgctccgg aatattctga cgcaggagtt ggatgtgatg
      181 gctgtgagcg cacaaatccg gcaatatatg gattattcct cgcgtttgca gttgctgcat
      241 tatctctttg gtattgccag cgccgacggc ggctttaatc tgaaagagat cggtatggcc
      301 gtgcagattg cccaattact gggaatccgc cagcaggata ttgaaagtgt gaaggcgatg
      361 tttgtcaggg atacccaaag tgcttacaaa attcttgaaa tccggcccga tgctacggat
      421 gaagacgtta agaaagccta ccggaaaatg gcgctcaaat atcatcccga ccgcgtagcc
      481 catctgggtg aagacgtaca gaaggctgcc aatgcaaaat tccaggaact gaatgccgct
      541 tatgaatccg ttaaaaagga gcggggaatg gtgtagatgt tgcctctatg tctcaatgcc
      601 gggcggtgat tgccgttggc tgtcagcctg ccgtctttcg ctcagcctgc cccggcaggt
      661 aaactgttag cttttagcgg ttggtcgttg gcttttggtc cttttattta gtgtttttca
      721 ttctccgtcc tccgctctcc ctgctcccgg cctttcccgg tcattctttc gcaattatgt
      781 gttccggatc tagaagatca agctttaata tttgttttaa tttattgtat attaaagggt
      841 tgtactcaaa tagctgcctg ggtttttcaa aatagcattc aatggcaacg gcaaaaaact
      901 cattgtagtt ttcagccccg tattcgcgaa aaaatgtctg gttcccggtt tcgtaattat
      961 tcatttcctg cactgcaagc tcatcgaatt catgtatcag tttggtcatc tcatcggatt
     1021 cccgttcctc gaaaaaggca taggaaagcc gcaaagcatg tgcggcctca tgaaggccca
     1081 ggttatgtcc gtcggattca ttttcaaggt cggccatcag gttcttccac gaaagtacga
     1141 tacagcctat cagattgact tccccttcat aatactggcc cgtccggctg gagtagtaat
     1201 cgtcggcgcg cagtataacc gttttaaaca tatccaggta ttgtgccgga tgaccgaagg
     1261 tgagctgcgt gaagatagcg ctgatgatga ctttcatttc ctctgttatt gtatcgatcc
     1321 cttcggaggc aacaaaattc ttcctggtca tgaaggtggc cagccgtcgc tcaaaccggc
     1381 gtttcagttt attattcaaa agcgggtaaa gcgatgaagc tttgttgatc atctcaacct
     1441 gggatgcagg caacagggat tcagtatctt tttggctaaa gaaacttaat ccggattttt
     1501 ctatccggac aaacgccggc ccgaagtgtg ttatcagcaa aacacccgca gccagaagaa
     1561 taagaatgaa tatgtaaatg gtaataccca taagtatgcc tggttttaag aataaggctt
     1621 ttaggagtac aaatatccgg gataattaca ctgaaacaat ggcttttagc agatatttta
//
LOCUS       DAOPYV010000142         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1305
            NODE_119426_length_1602_cov_8.489334, whole genome shotgun
            sequence.
ACCESSION   DAOPYV010000142 DAOPYV010000000
VERSION     DAOPYV010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854510
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:48:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,291
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119426_length_1602_cov_8.489334"
                     /isolate="AMR_MDS_1305"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PKI35_11600"
     CDS             complement(<1..931)
                     /locus_tag="PKI35_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNX45071.1"
                     /translation="MKRSKLLLISILFFSGLLVSAQSKPLTAEQKMKVFIDALMKKMT
                     LQEKIGQLNLSGAGDITTGQSSSSDIGKKIKEGKVGGILNLKSAEKIHDVQRVAVEES
                     RLKIPLIFGMDVIHGYETTFPIPLAMASSFDMSLVEKSARIAASEASADGICWTYSPM
                     VDIARDPRWGRIAEGAGEDPYLGSRVAEAFVKGYQGDDLTKNNTIMSCIKHFALYGAA
                     EAGRDYNTTDMSHVRMYNEYLPPYLAGVKAGAGSMMSSFNEIDGIPATANKWLMTDLL
                     RKQWGFKGFVVTDFTAINEMTAHGMGDLQTVSAL"
     gene            complement(983..>1602)
                     /locus_tag="PKI35_11605"
     CDS             complement(983..>1602)
                     /locus_tag="PKI35_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNX45072.1"
                     /translation="RTYWSAASSDENEWICSDLGALSTVYAVQVNYADQDAEFLGKSL
                     GVYHQYKIMESADGKKWNLLVDKSQNKTDVPHDYIELEKPVKTRFLKLVNIHMPTGKF
                     AISGFRAFGLGLGEKPGIVKEFIVLRTKKDKRSAWLRWSPVDNAYAYNIYMGTDPDKL
                     YNCIMVYNANEYWLKTMDKDKPYYFRIEAINENGVSLPSNVVLAE"
BASE COUNT          380 a          385 c          371 g          466 t
ORIGIN      
        1 cgagtgccga aacggtttgc aggtcgccca taccatgagc agtcatttcg ttgattgctg
       61 taaaatcggt caccacgaag cctttaaaac cccactgttt gcgcagcagg tcagtcataa
      121 gccacttatt tgccgttgcc gggattccgt caatttcatt gaacgatgac atcatgcttc
      181 ctgctccggc tttcacaccg gcaaggtagg gaggcaggta ctcattatac attcgtacgt
      241 ggctcatgtc ggtggtgttg tagtcgcggc cggcttcagc cgctccatac agggcaaaat
      301 gcttgataca cgacatgatg gtgttgtttt ttgtgaggtc atcgccctgg tagcctttaa
      361 caaatgcttc ggccactctt gaacccaggt acgggtcttc gcctgcacct tcggcaatgc
      421 ggccccagcg cgggtcacgg gcaatatcaa ccatgggaga gtatgtccag caaatgccgt
      481 cggcactggc ttcgctggca gcgatgcggg cgcttttctc tacaaggctc atatcgaagc
      541 tggatgccat agcgagcgga atcgggaacg tggtctcgta accatggatc acatccatcc
      601 cgaaaatcag gggaattttg aggcggcttt cttctacagc cacacgctgc acatcatgta
      661 tcttttcggc agatttaagg ttaaggattc ctcctacttt gccttctttt atctttttgc
      721 caatgtcgga actggaagat tgacctgtgg taatatcccc ggctcccgaa aggttcagct
      781 ggccgatttt ttcctgcagg gtcatctttt tcatcagtgc atcaatgaaa accttcattt
      841 tctgttctgc cgtcaacggt ttggattggg ctgaaaccag cagtccggag aagaataaga
      901 tgcttattaa cagcagttta cttcttttca tattttgtac ttttcttata taacaaggta
      961 aaaaattacg gacagcagga tgctattcag caagtacaac gtttgaaggc aaacttactc
     1021 cattctcgtt gattgcttca atcctgaaat aatatggctt gtccttatcc atggttttga
     1081 gccagtattc gttggcatta taaaccatga tgcaattata cagtttatcg ggatcggttc
     1141 ccatgtatat gttgtaggcg tatgcattat cgacggggct ccatcgcaac caggcgctgc
     1201 gcttgtcttt tttggtgcgc agaacaatga attctttcac aataccgggt ttttctccca
     1261 acccgagtcc gaatgccctg aaaccgctga ttgcaaattt gcctgttggc atatggatat
     1321 ttactagctt gagaaagcgg gttttaacag gtttttcaag ctcaatataa tcatggggaa
     1381 catctgtttt gttctggctt ttgtcaacca gcaggttcca ttttttaccg tcagccgatt
     1441 ccatgatctt atattgatga tacactccga gcgatttgcc aagaaattcg gcatcctggt
     1501 cagcataatt cacctggaca gcataaacag ttgataaagc acccagatca gagcagatcc
     1561 attcgttttc gtccgaagag gccgccgacc aataggtgcg ga
//
